LOCUS       DAPHDX010000001       109101 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPHDX010000001 DAPHDX010000000
VERSION     DAPHDX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.001"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..764)
                     /locus_tag="RH058_000001"
     CDS             complement(<1..764)
                     /locus_tag="RH058_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV3135650.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKMFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGIL"
     gene            complement(887..1339)
                     /gene="tabA"
                     /locus_tag="RH058_000002"
     CDS             complement(887..1339)
                     /gene="tabA"
                     /locus_tag="RH058_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV3135651.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1484..2077)
                     /locus_tag="RH058_000003"
     CDS             complement(1484..2077)
                     /locus_tag="RH058_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV3135652.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            2148..2861
                     /gene="bdcA"
                     /locus_tag="RH058_000004"
     CDS             2148..2861
                     /gene="bdcA"
                     /locus_tag="RH058_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3135653.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2992..3387
                     /locus_tag="RH058_000005"
     CDS             2992..3387
                     /locus_tag="RH058_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV3135654.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3668..3802
                     /gene="pyrL"
                     /locus_tag="RH058_000006"
     CDS             3668..3802
                     /gene="pyrL"
                     /locus_tag="RH058_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV3135655.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3806..4741
                     /gene="pyrB"
                     /locus_tag="RH058_000007"
     CDS             3806..4741
                     /gene="pyrB"
                     /locus_tag="RH058_000007"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV3135656.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4754..5215
                     /gene="pyrI"
                     /locus_tag="RH058_000008"
     CDS             4754..5215
                     /gene="pyrI"
                     /locus_tag="RH058_000008"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV3135657.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5288..5674
                     /gene="ridA"
                     /locus_tag="RH058_000009"
     CDS             5288..5674
                     /gene="ridA"
                     /locus_tag="RH058_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV3135658.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5880..8576)
                     /gene="mgtA"
                     /locus_tag="RH058_000010"
     CDS             complement(5880..8576)
                     /gene="mgtA"
                     /locus_tag="RH058_000010"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV3135659.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8717..8770)
                     /gene="mgtL"
                     /locus_tag="RH058_000011"
     CDS             complement(8717..8770)
                     /gene="mgtL"
                     /locus_tag="RH058_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV3135660.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8955..9902
                     /gene="treR"
                     /locus_tag="RH058_000012"
     CDS             8955..9902
                     /gene="treR"
                     /locus_tag="RH058_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV3135661.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10021..11442
                     /gene="treB"
                     /locus_tag="RH058_000013"
     CDS             10021..11442
                     /gene="treB"
                     /locus_tag="RH058_000013"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV3135662.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11492..13147
                     /gene="treC"
                     /locus_tag="RH058_000014"
     CDS             11492..13147
                     /gene="treC"
                     /locus_tag="RH058_000014"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV3135663.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13541..15679
                     /gene="nrdD"
                     /locus_tag="RH058_000015"
     CDS             13541..15679
                     /gene="nrdD"
                     /locus_tag="RH058_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV3135664.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVKAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15837..16301
                     /gene="nrdG"
                     /locus_tag="RH058_000016"
     CDS             15837..16301
                     /gene="nrdG"
                     /locus_tag="RH058_000016"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV3135665.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16346..16732)
                     /gene="cybC"
                     /locus_tag="RH058_000017"
     CDS             complement(16346..16732)
                     /gene="cybC"
                     /locus_tag="RH058_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV3135666.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16915..18267)
                     /gene="pmbA"
                     /locus_tag="RH058_000018"
     CDS             complement(16915..18267)
                     /gene="pmbA"
                     /locus_tag="RH058_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV3135667.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18361..18912
                     /locus_tag="RH058_000019"
     CDS             18361..18912
                     /locus_tag="RH058_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV3135668.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19063..20436)
                     /gene="mpl"
                     /locus_tag="RH058_000020"
     CDS             complement(19063..20436)
                     /gene="mpl"
                     /locus_tag="RH058_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV3135669.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20612..21610
                     /gene="fbp"
                     /locus_tag="RH058_000021"
     CDS             20612..21610
                     /gene="fbp"
                     /locus_tag="RH058_000021"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV3135670.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21643..22638)
                     /gene="yjfF"
                     /locus_tag="RH058_000022"
     CDS             complement(21643..22638)
                     /gene="yjfF"
                     /locus_tag="RH058_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV3135671.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22625..23647)
                     /locus_tag="RH058_000023"
     CDS             complement(22625..23647)
                     /locus_tag="RH058_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3135672.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(23661..25163)
                     /locus_tag="RH058_000024"
     CDS             complement(23661..25163)
                     /locus_tag="RH058_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3135673.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25473..26429)
                     /gene="ytfQ"
                     /locus_tag="RH058_000025"
     CDS             complement(25473..26429)
                     /gene="ytfQ"
                     /locus_tag="RH058_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV3135674.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26739..27269
                     /gene="ppa"
                     /locus_tag="RH058_000026"
     CDS             26739..27269
                     /gene="ppa"
                     /locus_tag="RH058_000026"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV3135675.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27349..27699)
                     /gene="chpB"
                     /locus_tag="RH058_000027"
     CDS             complement(27349..27699)
                     /gene="chpB"
                     /locus_tag="RH058_000027"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDV3135676.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(27693..27944)
                     /gene="chpS"
                     /locus_tag="RH058_000028"
     CDS             complement(27693..27944)
                     /gene="chpS"
                     /locus_tag="RH058_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDV3135677.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(28156..28497)
                     /locus_tag="RH058_000029"
     CDS             complement(28156..28497)
                     /locus_tag="RH058_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV3135678.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28500..32279)
                     /gene="tamB"
                     /locus_tag="RH058_000030"
     CDS             complement(28500..32279)
                     /gene="tamB"
                     /locus_tag="RH058_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV3135679.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGILSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32276..34009)
                     /gene="tamA"
                     /locus_tag="RH058_000031"
     CDS             complement(32276..34009)
                     /gene="tamA"
                     /locus_tag="RH058_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV3135680.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34215..34853
                     /gene="msrA"
                     /locus_tag="RH058_000032"
     CDS             34215..34853
                     /gene="msrA"
                     /locus_tag="RH058_000032"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV3135681.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35176..36519
                     /locus_tag="RH058_000033"
     CDS             35176..36519
                     /locus_tag="RH058_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV3135682.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36581..36787)
                     /locus_tag="RH058_000034"
     CDS             complement(36581..36787)
                     /locus_tag="RH058_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV3135683.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37112..37669
                     /locus_tag="RH058_000035"
     CDS             37112..37669
                     /locus_tag="RH058_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV3135684.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37659..38399)
                     /gene="cysQ"
                     /locus_tag="RH058_000036"
     CDS             complement(37659..38399)
                     /gene="cysQ"
                     /locus_tag="RH058_000036"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV3135685.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38589..40532
                     /gene="cpdB"
                     /locus_tag="RH058_000037"
     CDS             38589..40532
                     /gene="cpdB"
                     /locus_tag="RH058_000037"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV3135686.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDAKTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     SDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40661..41041)
                     /locus_tag="RH058_000038"
     CDS             complement(40661..41041)
                     /locus_tag="RH058_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV3135687.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41130..41990
                     /gene="qorB"
                     /locus_tag="RH058_000039"
     CDS             41130..41990
                     /gene="qorB"
                     /locus_tag="RH058_000039"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV3135688.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            42098..43063
                     /locus_tag="RH058_000040"
     CDS             42098..43063
                     /locus_tag="RH058_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3135689.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            43171..43833
                     /gene="ytfE"
                     /locus_tag="RH058_000041"
     CDS             43171..43833
                     /gene="ytfE"
                     /locus_tag="RH058_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV3135690.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43878..45290)
                     /gene="cycA"
                     /locus_tag="RH058_000042"
                     /gene_synonym="dagA"
     CDS             complement(43878..45290)
                     /gene="cycA"
                     /locus_tag="RH058_000042"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV3135691.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45599..46219)
                     /gene="fklB"
                     /locus_tag="RH058_000043"
     CDS             complement(45599..46219)
                     /gene="fklB"
                     /locus_tag="RH058_000043"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3135692.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46438..47076
                     /gene="ytfB"
                     /locus_tag="RH058_000044"
     CDS             46438..47076
                     /gene="ytfB"
                     /locus_tag="RH058_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV3135693.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDMPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(47060..47707)
                     /locus_tag="RH058_000045"
     CDS             complement(47060..47707)
                     /locus_tag="RH058_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDV3135694.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47920..48711
                     /locus_tag="RH058_000046"
     CDS             47920..48711
                     /locus_tag="RH058_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDV3135695.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            48721..49563
                     /locus_tag="RH058_000047"
     CDS             48721..49563
                     /locus_tag="RH058_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV3135696.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            49573..50349
                     /locus_tag="RH058_000048"
     CDS             49573..50349
                     /locus_tag="RH058_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDV3135697.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            50359..51900
                     /locus_tag="RH058_000049"
     CDS             50359..51900
                     /locus_tag="RH058_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV3135698.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRYLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            51897..53978
                     /locus_tag="RH058_000050"
     CDS             51897..53978
                     /locus_tag="RH058_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDV3135699.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNINGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            53999..54475
                     /locus_tag="RH058_000051"
     CDS             53999..54475
                     /locus_tag="RH058_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV3135700.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            54518..55819
                     /locus_tag="RH058_000052"
     CDS             54518..55819
                     /locus_tag="RH058_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDV3135701.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            55872..56843
                     /locus_tag="RH058_000053"
     CDS             55872..56843
                     /locus_tag="RH058_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV3135702.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(56955..57404)
                     /gene="rplI"
                     /locus_tag="RH058_000054"
     CDS             complement(56955..57404)
                     /gene="rplI"
                     /locus_tag="RH058_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV3135703.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(57446..57673)
                     /gene="rpsR"
                     /locus_tag="RH058_000055"
     CDS             complement(57446..57673)
                     /gene="rpsR"
                     /locus_tag="RH058_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV3135704.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(57678..57992)
                     /gene="priB"
                     /locus_tag="RH058_000056"
     CDS             complement(57678..57992)
                     /gene="priB"
                     /locus_tag="RH058_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV3135705.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(57999..58394)
                     /gene="rpsF"
                     /locus_tag="RH058_000057"
     CDS             complement(57999..58394)
                     /gene="rpsF"
                     /locus_tag="RH058_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV3135706.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            58721..58996
                     /locus_tag="RH058_000058"
     CDS             58721..58996
                     /locus_tag="RH058_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3135707.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(59125..59811)
                     /gene="ulaF"
                     /locus_tag="RH058_000059"
     CDS             complement(59125..59811)
                     /gene="ulaF"
                     /locus_tag="RH058_000059"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV3135708.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(59811..60665)
                     /gene="ulaE"
                     /locus_tag="RH058_000060"
     CDS             complement(59811..60665)
                     /gene="ulaE"
                     /locus_tag="RH058_000060"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV3135709.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(60675..61325)
                     /gene="ulaD"
                     /locus_tag="RH058_000061"
     CDS             complement(60675..61325)
                     /gene="ulaD"
                     /locus_tag="RH058_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV3135710.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(61339..61803)
                     /gene="ulaC"
                     /locus_tag="RH058_000062"
     CDS             complement(61339..61803)
                     /gene="ulaC"
                     /locus_tag="RH058_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV3135711.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(61813..62118)
                     /gene="ulaB"
                     /locus_tag="RH058_000063"
     CDS             complement(61813..62118)
                     /gene="ulaB"
                     /locus_tag="RH058_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV3135712.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(62134..63531)
                     /gene="ulaA"
                     /locus_tag="RH058_000064"
     CDS             complement(62134..63531)
                     /gene="ulaA"
                     /locus_tag="RH058_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV3135713.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            63886..64950
                     /gene="ulaG"
                     /locus_tag="RH058_000065"
     CDS             63886..64950
                     /gene="ulaG"
                     /locus_tag="RH058_000065"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV3135714.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            65058..65813
                     /gene="ulaR"
                     /locus_tag="RH058_000066"
     CDS             65058..65813
                     /gene="ulaR"
                     /locus_tag="RH058_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV3135715.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(65810..66559)
                     /gene="yjfP"
                     /locus_tag="RH058_000067"
     CDS             complement(65810..66559)
                     /gene="yjfP"
                     /locus_tag="RH058_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3135716.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            66741..67070
                     /gene="bsmA"
                     /locus_tag="RH058_000068"
     CDS             66741..67070
                     /gene="bsmA"
                     /locus_tag="RH058_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV3135717.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            67219..67494
                     /gene="yjfN"
                     /locus_tag="RH058_000069"
     CDS             67219..67494
                     /gene="yjfN"
                     /locus_tag="RH058_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV3135718.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(67611..69236)
                     /locus_tag="RH058_000070"
     CDS             complement(67611..69236)
                     /locus_tag="RH058_000070"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV3135719.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(69320..70483)
                     /locus_tag="RH058_000071"
     CDS             complement(69320..70483)
                     /locus_tag="RH058_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV3135720.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(70486..71124)
                     /locus_tag="RH058_000072"
     CDS             complement(70486..71124)
                     /locus_tag="RH058_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV3135721.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(71134..71532)
                     /locus_tag="RH058_000073"
     CDS             complement(71134..71532)
                     /locus_tag="RH058_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV3135722.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(71550..72209)
                     /locus_tag="RH058_000074"
     CDS             complement(71550..72209)
                     /locus_tag="RH058_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV3135723.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(72260..72958)
                     /locus_tag="RH058_000075"
     CDS             complement(72260..72958)
                     /locus_tag="RH058_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV3135724.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(72977..73378)
                     /locus_tag="RH058_000076"
     CDS             complement(72977..73378)
                     /locus_tag="RH058_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV3135725.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(73505..74236)
                     /gene="rlmB"
                     /locus_tag="RH058_000077"
     CDS             complement(73505..74236)
                     /gene="rlmB"
                     /locus_tag="RH058_000077"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV3135726.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(74416..76857)
                     /gene="rnr"
                     /locus_tag="RH058_000078"
     CDS             complement(74416..76857)
                     /gene="rnr"
                     /locus_tag="RH058_000078"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV3135727.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(76896..77321)
                     /gene="nsrR"
                     /locus_tag="RH058_000079"
     CDS             complement(76896..77321)
                     /gene="nsrR"
                     /locus_tag="RH058_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV3135728.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(77526..78824)
                     /gene="purA"
                     /locus_tag="RH058_000080"
     CDS             complement(77526..78824)
                     /gene="purA"
                     /locus_tag="RH058_000080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV3135729.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(78928..79125)
                     /locus_tag="RH058_000081"
     CDS             complement(78928..79125)
                     /locus_tag="RH058_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV3135730.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(79207..80211)
                     /gene="hflC"
                     /locus_tag="RH058_000082"
     CDS             complement(79207..80211)
                     /gene="hflC"
                     /locus_tag="RH058_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV3135731.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(80214..81473)
                     /gene="hflK"
                     /locus_tag="RH058_000083"
     CDS             complement(80214..81473)
                     /gene="hflK"
                     /locus_tag="RH058_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV3135732.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(81559..82839)
                     /gene="hflX"
                     /locus_tag="RH058_000084"
     CDS             complement(81559..82839)
                     /gene="hflX"
                     /locus_tag="RH058_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV3135733.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(82916..83224)
                     /gene="hfq"
                     /locus_tag="RH058_000085"
     CDS             complement(82916..83224)
                     /gene="hfq"
                     /locus_tag="RH058_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV3135734.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(83310..84260)
                     /gene="miaA"
                     /locus_tag="RH058_000086"
     CDS             complement(83310..84260)
                     /gene="miaA"
                     /locus_tag="RH058_000086"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV3135735.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(84253..86100)
                     /gene="mutL"
                     /locus_tag="RH058_000087"
     CDS             complement(84253..86100)
                     /gene="mutL"
                     /locus_tag="RH058_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV3135736.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(86110..87447)
                     /gene="amiB"
                     /locus_tag="RH058_000088"
     CDS             complement(86110..87447)
                     /gene="amiB"
                     /locus_tag="RH058_000088"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV3135737.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(87466..87927)
                     /gene="tsaE"
                     /locus_tag="RH058_000089"
     CDS             complement(87466..87927)
                     /gene="tsaE"
                     /locus_tag="RH058_000089"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV3135738.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(87899..89446)
                     /gene="nnr"
                     /locus_tag="RH058_000090"
     CDS             complement(87899..89446)
                     /gene="nnr"
                     /locus_tag="RH058_000090"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV3135739.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            89445..90584
                     /gene="queG"
                     /locus_tag="RH058_000091"
     CDS             89445..90584
                     /gene="queG"
                     /locus_tag="RH058_000091"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV3135740.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(90567..90620)
                     /locus_tag="RH058_000092"
     CDS             complement(90567..90620)
                     /locus_tag="RH058_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3135741.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(90854..90929)
                     /locus_tag="RH058_000093"
     tRNA            complement(90854..90929)
                     /locus_tag="RH058_000093"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90894..90896),aa:Gly,seq:gcc)
     gene            complement(90966..91041)
                     /locus_tag="RH058_000094"
     tRNA            complement(90966..91041)
                     /locus_tag="RH058_000094"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91006..91008),aa:Gly,seq:gcc)
     gene            complement(91252..91797)
                     /gene="orn"
                     /locus_tag="RH058_000095"
     CDS             complement(91252..91797)
                     /gene="orn"
                     /locus_tag="RH058_000095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV3135742.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            91892..92944
                     /gene="rsgA"
                     /locus_tag="RH058_000096"
     CDS             91892..92944
                     /gene="rsgA"
                     /locus_tag="RH058_000096"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV3135743.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            93041..94009
                     /gene="psd"
                     /locus_tag="RH058_000097"
     CDS             93041..94009
                     /gene="psd"
                     /locus_tag="RH058_000097"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV3135744.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            94031..97354
                     /gene="mscM"
                     /locus_tag="RH058_000098"
     CDS             94031..97354
                     /gene="mscM"
                     /locus_tag="RH058_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV3135745.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(97383..97697)
                     /locus_tag="RH058_000099"
     CDS             complement(97383..97697)
                     /locus_tag="RH058_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV3135746.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(97694..98008)
                     /locus_tag="RH058_000100"
     CDS             complement(97694..98008)
                     /locus_tag="RH058_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3135747.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(98060..99562)
                     /gene="yjeM"
                     /locus_tag="RH058_000101"
     CDS             complement(98060..99562)
                     /gene="yjeM"
                     /locus_tag="RH058_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV3135748.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(99781..100758)
                     /gene="epmA"
                     /locus_tag="RH058_000102"
     CDS             complement(99781..100758)
                     /gene="epmA"
                     /locus_tag="RH058_000102"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV3135749.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            101083..102891
                     /gene="frdA"
                     /locus_tag="RH058_000103"
     CDS             101083..102891
                     /gene="frdA"
                     /locus_tag="RH058_000103"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV3135750.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            102884..103618
                     /gene="frdB"
                     /locus_tag="RH058_000104"
     CDS             102884..103618
                     /gene="frdB"
                     /locus_tag="RH058_000104"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV3135751.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            103629..104024
                     /gene="frdC"
                     /locus_tag="RH058_000105"
     CDS             103629..104024
                     /gene="frdC"
                     /locus_tag="RH058_000105"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV3135752.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            104035..104394
                     /gene="frdD"
                     /locus_tag="RH058_000106"
     CDS             104035..104394
                     /gene="frdD"
                     /locus_tag="RH058_000106"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV3135753.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            104457..105590
                     /locus_tag="RH058_000107"
     CDS             104457..105590
                     /locus_tag="RH058_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV3135754.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            105679..106212
                     /gene="blc"
                     /locus_tag="RH058_000108"
     CDS             105679..106212
                     /gene="blc"
                     /locus_tag="RH058_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV3135755.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(106209..106526)
                     /gene="sugE"
                     /locus_tag="RH058_000109"
     CDS             complement(106209..106526)
                     /gene="sugE"
                     /locus_tag="RH058_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3135756.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(106708..106854)
                     /gene="ecnB"
                     /locus_tag="RH058_000110"
     CDS             complement(106708..106854)
                     /gene="ecnB"
                     /locus_tag="RH058_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV3135757.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(106965..107090)
                     /gene="ecnA"
                     /locus_tag="RH058_000111"
     CDS             complement(106965..107090)
                     /gene="ecnA"
                     /locus_tag="RH058_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV3135758.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(107142..107708)
                     /gene="efp"
                     /locus_tag="RH058_000112"
     CDS             complement(107142..107708)
                     /gene="efp"
                     /locus_tag="RH058_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV3135759.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            107750..108778
                     /gene="epmB"
                     /locus_tag="RH058_000113"
     CDS             107750..108778
                     /gene="epmB"
                     /locus_tag="RH058_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV3135760.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLHQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT        26613 a        29169 c        27290 g        26029 t
ORIGIN      
        1 ttcagtatcc ctttttcacc aagcaagtat gaaatcgatt cattacatac ataaggcatt
       61 aatagcagat gttctttcct tattgctaat aatgcattat tcaatggttt ttctaataaa
      121 tccgtaatcg tagaatcaac tgaatcttta caactcacct gcgagttatt caagtcctga
      181 gaattattta actcctgcga aatatttaat agacgagcta tttccttgtt agcttcgcgt
      241 tggtaacacg ccagaatctt gttagtaatc aggtgtaatg aactctcatc ttttctttca
      301 aaggacttcc cttcataacg attagtcgaa ccatcaggtt gtggaaatat aatgactgta
      361 tgtccttcac gaatcccatt attaaaagct gaggcaagtg taatttctat ttccacctta
      421 gataaatccg ctcgctcccc ttcaggatta ttcataacaa acttcattat ctcactacca
      481 tcattatttt tgatataaga tgaggactta atatgagcca tcatatttct acccgcttcg
      541 ttggagcgtt tgctaaaatc cacataaccc agggtaaaca tcttagccaa aaactccaaa
      601 aatgctctgg gagaaagaat attgcttctg ctattctgcc ctccggcaat tcgggcaaaa
      661 tcattcattc ccacatacca ggtatctaag tcttcatttt tttgtttaca attgtcggct
      721 gatgtaatgt tttgagaaaa attccaacct gatattttgc tcatgacgga ctccttgagt
      781 taataagaac gtctaagcat tacaaattct actcacaaaa aaatatcaaa aaacgctctt
      841 ttcacaaact cgccgtaaac ctggagttat ttacggcgaa aagtcatcac gccatcagca
      901 tcttcactac tgctttgcga acctgggctg gcgcgcccac tgcgcacagc ggtttatgca
      961 cttcccccgg ataaaacaca acaaaatcac cttcattaag gataactgtt ttctcatcaa
     1021 cgccttccgg caaaaatgcg atgtctttat cagctaacca gtcggtatcc ggcgtgcctg
     1081 caggttgcgt gctgaaggtc atgccttcct gaccttttaa cacaatctga atgtcgagat
     1141 agcgggcatg atactccgca cggcgagctt cgtacggctc ggtcatatct tccgagataa
     1201 gataaaacag acgattgcct tcgatatcgt gcttgccctt tggcgtttct accgtaacgt
     1261 gtgctttgat atgctcaatc gcctggcgta actcctgggg tagccacggc tgaagattat
     1321 gaatatttcc gatgatcatc ttttccactc cttaattaaa acaacgtttc atttctatcg
     1381 ttatacgaaa agatgatcaa ttccgctagc cttaacgcgt gaattattgt cttatcacaa
     1441 cttatcggca ggcgcgttcc cgcctttcgg gtcaaggccc gcatcactcc ttgagaatag
     1501 tttttatcgc tccccccgcc agtgcagctg ttgcacagag ttgttcgagg gagtgcccct
     1561 ctcgtgcctt cgcagaaagc cctgacatca cggtactcat aaaatcagtc acacattgtg
     1621 cgctttgtgg atgccgcctg gcgatatagt cataaatggc cgtttctgcg gcgtgataat
     1681 attgaacggc aatatcacgc gcttgtggat catgactatg aatgccttca agaaccatac
     1741 agccagcgca gccgccgttt tggctatatc tgcgcgccgc ttcttttaat acctcagcca
     1801 ggcactcccc tactggacga tcatcacgaa gaatatcgac aagcggaata gcttccgtac
     1861 cgacatattc attgagtaca cggctaaata acccagcctt actgccaaaa gccgcgtaga
     1921 ggctcggggg gttaatacca agataatcag taacttcagc aacactgaca gcatcgaaac
     1981 ctttttggtg aaaaagcact tttgccgcag agactgcctg ctcaggagcg aaacgtctgg
     2041 gacgacctgg aacgcgagat tgttttttag tgaccattac aaaacttgtt gacagaaagt
     2101 taaaacagtt ttgtaatgca tgttacataa taaatcaagg agtccttatg ggcgctttta
     2161 caggtaagac agttctcatc ctcggtggta gccgtggaat cggtgccgct atcgtacgtc
     2221 gtttcgtcac cgatggggcc aatgtacgat tcacctatgc ggggtcgaaa gatgccgctg
     2281 aacgcctggc acaagagact ggagcaacag cagtattcac agatagtgct gacagagacg
     2341 ctgttattga tgtcgttcgt aagagcggcg cattggatat cctcgtggta aatgcaggta
     2401 ttggcgtctt tggcgatgcc ctggaattaa atgccgacga tattgatcgc cttttcaaaa
     2461 tcaatattca tgctccttac catgcctccg ttgaagccgc ccggcagatg cccgaaggcg
     2521 ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat gcctgttgca ggcatggctg
     2581 cttatgccgc cagcaaatct gccctgcaag gcatggcgcg cgggctggcc cgtgattttg
     2641 gaccgcgtgg gatcaccatc aacgtcgtcc agccagggcc aattgatacc gacgctaatc
     2701 ccgccaacgg gccaatgcgc gatatgttgc atggttttat ggctatcaaa agacatgggc
     2761 aaccggaaga ggtcgctggt atggtcgcat ggttagcagg gccagaagcc agctttgtta
     2821 ccggcgcgat gcataccatt gatggcgcgt ttggcgcata accgactacg ctcaattaag
     2881 cccagccatt tcccatgatg tctgggtttt gtttactcac gtcgtccgct aaaagcggct
     2941 cctggtaaat ataaatcttc tgttcatgtc gtaaatcttg aggataacca aatggtagaa
     3001 agaaccgctg ttttccctgc tggccgacat tcactgtatg ctgaacatcg ttattctgcg
     3061 gctattcgct ccggcgattt gctgtttgtt tccgggcaag taggaagtcg agaggacgga
     3121 acaccagaac ccgattttca gcaacaagtc agactggcat ttgataattt gcatgcgacc
     3181 ctggctgctg cgggatgcac ttttgacgat atcattgatg ttacgagctt tcataccgat
     3241 ccagaaaaac aattcgaaga catcatgacg gtgaaaaatg aaatatttag cgccccacct
     3301 tatccaaact ggacggcggt aggtgttaca tggctggcag gctttgattt tgaaattaaa
     3361 gtgatagcgc gcatccctga gcagtaagca atagtgttag ccgttcgttt tcacactccg
     3421 ccctataagt cggatgaatg gaataaaatg catatctgat tgcgtgaaag tgaaaaagga
     3481 aaaagcaggg aatgtctgca attattgata ccgaaggaca gttcccctgc agaatcacat
     3541 caaataaaaa tgcatatacc ttgactttta attcaaataa accgtttgcg ctgacaaaat
     3601 attgcatcaa atgcttgcac cgcttctgac gatgagtata atgccggaca atttgccggg
     3661 aggatgtatg gttcagtgtg ttcgacattt tgtcttaccg cgtctgaaaa aagacgctgg
     3721 cctgccgttt ttcttcccgt tgatcaccca ttcccagccc ctcaatcgag gggctttttt
     3781 ttgcccaggc gtcaggagat aaaagatggc taatccgcta tatcagaaac atatcatttc
     3841 cataaacgac cttagtcgcg atgaccttaa tctggtgctg gcgacagcgg cgaaactgaa
     3901 agcaaacccg caaccagagc tgttgaagca caaagtcatt gccagctgtt tcttcgaagc
     3961 ctctacccgt acccgcctct ctttcgaaac atctatgcac cgcctgggtg ccagcgtggt
     4021 gggcttctcc gacagcgcca atacatcact gggtaaaaag ggcgaaacgc tggccgatac
     4081 catttcggtt atcagcactt acgtcgatgc gatagtgatg cgtcatccgc aggaaggtgc
     4141 ggcgcgcctg gccaccgagt tttccggcaa tgtaccggta ctgaatgccg gtgatggctc
     4201 caaccaacat ccgacgcaaa ccttgctgga cttattcact attcaggaaa cccaggggcg
     4261 tctggacaat ctccacgtcg caatggttgg tgacctgaaa tatggccgca ccgttcactc
     4321 cctgactcag gcgttagcga agttcgacgg caaccgtttt tacttcatcg cgccggacgc
     4381 gctggcaatg ccgcaataca ttctggatat gctcgatgaa aaagggatcg catggagtct
     4441 gcacagctct attgaagaag tgatggcgga agtagacatc ctgtacatga cccgcgtgca
     4501 aaaagagcgt ctggacccgt ccgagtacgc caacgtgaaa gcgcagtttg ttcttcgcgc
     4561 cagcgatctc cacaacgcca aagccaatat gaaagtgctg catccgctgc cgcgtgttga
     4621 tgagattgcg acggatgttg ataaaacgcc acacgcctgg tacttccagc aggcaggcaa
     4681 cgggattttc gctcgccagg cgttactggc actggttctg aatcgcgatc tggtactgta
     4741 aggggaaata gagatgacac acgataataa attgcaggtt gaagctatta aacgcggcac
     4801 ggtaattgac catatccccg cccagatcgg ttttaagctg ttgagtctgt tcaagctgac
     4861 cgaaacggat cagcgcatca ccatcggtct gaacttgcct tctggcgaga tgggccgcaa
     4921 agatctgatc aaaatcgaaa ataccttttt gagtgaagat caggtagatc aactggcatt
     4981 gtatgcgccg caagccacgg ttaaccgtat cgacaactat gaagtggtgg gtaaatcgcg
     5041 ccccagtctg ccagagcgca tcgacaatgt gctggtctgc ccgaacagca actgtatcag
     5101 ccatgccgaa ccggtttctt ccagctttgc cgtgcgaaaa cgcgccaatg acatcgcgct
     5161 caaatgcaaa tactgtgaaa aagagttttc ccataatgtg gtgctggcca attaattgcg
     5221 gttggtaata aaagtctggc tccctataat gagccagact ttttaccgct gtaataaagg
     5281 agaaatcatg agcaaaacta tcgcgacgga aaatgcaccg gcagctatcg gtccttacgt
     5341 acagggcgtt gatctgggta atatgatcat cacctccggc cagatcccgg taaatccgaa
     5401 aacgggcgaa gtaccggcag acgtcgctgc acaggcacgt cagtcgctgg ataacgtaaa
     5461 agcgatcgtc gaagccgcag gcctgaaagt gggcgacatc gttaaaacca ccgtgtttgt
     5521 gaaagatctg aacgacttcg caaccgttaa tgctacctac gaagccttct tcaccgaaca
     5581 caatgccacc ttcccggcac gttcttgcgt tgaagttgcc cgtctgccga aagacgtgaa
     5641 aattgagatc gaagcgatcg ctgttcgtcg ctaatcttga tggaaatccg ggctatcatg
     5701 cccggattaa gtctgatgac aaacacaaaa tcgcctgatg cgctacgctt atcaggccta
     5761 cgtgattcct gcaatttatt gaatttgttg gccggataag gcatttacgc cgcatccggc
     5821 atgaacaaaa ctcactttgt ctgcaattcg aatccaggcc accatgccca gttgattctt
     5881 tattgccagc cgtaacgacg gctataaaat cctttcacca actgggttaa tgtcatatac
     5941 cctgccagaa tcgcaaccag ccacgggaaa tagcttaacg gcagcgcctg cagttgcaga
     6001 taactggcca gcggtgaaaa cggcaatgcg atcccgacaa tcatcacgat cacggtcatg
     6061 atcattaacg gccacgatgc acagctctga ataaacggca cacggcgggt gcggatcata
     6121 tgcacaatca gcgtttgcga cagtaagccc accacaaacc atcccgactg gaacagcgtt
     6181 tgcgtttccg gcgtgttggc atggaatacc caccacatca ggcaaaacgt cagaatatcg
     6241 aagatcgagc tgatcggtcc gaagaagatc ataaagcgcc ccagatccgc cggattccaa
     6301 cgctgcggct tttgaatttg ctcgtcgtcg acgttatcaa acgggatcgc cacctgtgac
     6361 acatcataca gcaggttctg aataagcaag tgtaacggca acatcggcag gaagggcaag
     6421 aaagcactcg ccaccagcac gctgaacaca ttaccgaagt tggagctcgc cgtcattttg
     6481 atgtatttca gcatgttggc gaaagtgcga cgtccttcaa taaccccctc ttccagcacc
     6541 atcaggcttt tttccagcag gatgatatca gccgcttcac gggcaatatc taccgcgccg
     6601 tccacagaaa tgccgatatc cgcagcgcgt aaggcgggcg catcattaat accatcgccc
     6661 ataaagccaa ccacatgccc ttcgcgcttc agtaaggtca caatgcgttc tttatgcatc
     6721 ggcgtcaggc gggcaaacag cgtggtgcgc tgtgcgagat ttgccagttc gtcgtcagat
     6781 agcgtttcaa tatcactacc aatgaccacc tctcccgcgt ccagccccac ttcatggcac
     6841 actttcgctg caactaactc actgtcgcca gtaaggattt ttacggtgat tccactcgct
     6901 tttaatgcct tcagtgccgg agcggttgtc tcttttggcg gatcaaggaa agcaatatat
     6961 ccttcgagga tcaggtcgga ttcatccgcc cgctggtaat ctccttcacg cgctggcagg
     7021 tatttcgtcg ccaccgcaac cacgcgcagc ccctgacgat taagcgtatc agtaacccgc
     7081 ttaatcttac gcagcatgat gtcatcgagc ggtacaatct cgccattgtg acgcacctgc
     7141 gaacacacat tgaggatttc ctgcaatgca cctttgcaaa ccagctgatg gtgctcggta
     7201 ttttctgcca ctaccaccga catccggcga cgctcgaaat caaacggaat ctcatcaatt
     7261 ttttgccaac gactggccag cgagcgcgct gactcttcat ccgtaccttc gagcaccgct
     7321 gtatcgagca ggtttttaag tccggtctga taatgactgt tcaaccacgc gctatgcagc
     7381 acgcgttcgc tggttttacc ggagatatcg gtatgattct ccagcacaat tttgtcctgt
     7441 gtcagggtgc cagttttatc agtgcacaga atatccattg cgccaaagtt ctgaatagca
     7501 tccagatgtt tgacgatcac tttctgtttc gacagcttta ctgcgccgcg cgccagcgtc
     7561 gaggtaacaa tcatcggcaa catttccggc gttaagccta ccgcgaccga aagcgcaaac
     7621 agtgccgctt cccaccagtc gcctttggtg taaccgttga taagcaacac caccggagcc
     7681 atcaccagca taaagcgaat cagcagcata ctgacgcggc tgatcccctg ctgaaaggca
     7741 ttcggctcgc tttcctgctc actaacacgc cccgccagtt gaccaaacca ggtattggca
     7801 cctgtagcaa tcaccattgc ttgtgccgtg ccgctcacca cggtggtgcc cataaaacac
     7861 agcgtgtcgc actccagcgg attgctgtgc tccggctggc gagtggtagc ggctttttcc
     7921 acgggcagag actcaccggt taacgacgcc tgagcgacga acagatcccg cgcctgcaag
     7981 atacgtaaat ctgccgggat catatctccc gccgccagtt ttataatatc gccgggcacc
     8041 agctggtcga tcgggatctc cagccagcca ttttcgcctt tgtcgttaat tacgcgcagc
     8101 accgtcgcgg tattgctgac catcgctttc agggcatctg ccgctttagt ggaacgtgct
     8161 tcctgaataa agttcagtaa cgttgaaata gcgaccatta gcgcaataac gcccgcggca
     8221 aataaatctt ccgtggcgta agaaatagcg ccgagaatgg tgagtaaaat attaaatggg
     8281 ttgcgatagc agacccataa atgtacccac cacggcgacg gttgttgtgc gggtaattta
     8341 ttttcaccat gttgttcgcg ggcagattcc acttccgcct gatttaaccc ttccggatgg
     8401 gtgtcgaacg ttttccataa ttcttcttcg ggcatcaccg ccattttcag gcaatgcgca
     8461 cttaaggacg gcgggaccac cgtattcact gtctgctgcg cgccaggcaa tggatcacga
     8521 tgaaccagac gggaaggtaa atggcgaatg agccgggtaa agatttcttt aaacataagg
     8581 agtccctccg cactgtctga aacagtacag aaatgtcttc aggcgtggcg aagccttacc
     8641 tgaaggtaat aaagctgagc agggacgtta aaacgtcacg gccttacgca tccggtaacg
     8701 ccgtgaaaag acgggcttac cggaaaagtt ttaatctccg tcgagggaga ggcgtgggat
     8761 caggttccat ataacctccg gtaagtgaat aaagcgccgc aaataataag gagaatccaa
     8821 taatattcgt agcatctatg gcgatataat acctgctggc aactaaacca acgataaacc
     8881 agactttacc cattgctgaa tgcacgggta aagttaggct caaataatta aacaacacgt
     8941 tacaggacaa caggatgcaa aatcggctga ccatcaaaga catcgcacgc ttaagcggcg
     9001 tggggaaatc tacagtttcc cgggtgctga ataacgaaag cggcgtgagc cagcgcaccc
     9061 gcgagcgtgt tgaagcagtg atgaatcagc atggattttc cccttcccgc tctgcgcgcg
     9121 ctatgcgtgg gcaaagcgat aaagtggtcg ccatcattgt tacccgcctg gattcgttgt
     9181 cagaaaatct cgccgttcaa accatgctgc cagcgttcta tgaacaaggt tacgacccaa
     9241 tcatgatgga aagtcagttt tccccgcaat tagttgccga acatttgggg gtgctgaaac
     9301 ggcgtaatat cgacggcgta gtgctgttcg gttttactgg cataacagaa gaaatgttag
     9361 cccactggca gtcatcgctg gttctgctgg cgcgtgacgc aaaaggtttt gcttcggtct
     9421 gttatgacga cgaaggggca atcaagatcc tgatgcaacg gctgtatgac caggggcatc
     9481 gtaatatcag ttatctcggc gtgccgcata gtgacgtgac gaccgggaag cgacgtcacg
     9541 aagcctacct ggcgttctgc aaagcgcata aactgcatcc cgttgccgct ctgccaggcc
     9601 ttgctatgaa gcaaggctat gagaacgtag caaaagtgat tacgcctgaa actaccgcct
     9661 tactgtgcgc aaccgacacg ctggcacttg gcgcaagtaa atacctgcaa gagcaacgca
     9721 tcgacacctt gcaactggcg agcgtcggta atacgccatt aatgaaattc ctccatccgg
     9781 agatcgtcac cgtagatcct ggttacgccg aagctggacg ccaggcggcc tgccaattga
     9841 tcgcacaggt aaccgggcgc agcgaaccgc aacaaatcat catccccgcc accctgtcct
     9901 gatcgtttcc tgaacgataa attgtgatct tcgctgcgtt tcgggaacgt tcccgttttt
     9961 aaatttttcc gcgcaatata ttctgcagcc aaccaaaaat gtcatctgcc atggggcttt
    10021 atgatgagca aaataaacca aacggatatc gatcggttga ttgaactggt cggcgggcgc
    10081 ggtaatattg cgacggtgag ccactgtatt actcgcctgc gctttgtcct caaccaaccg
    10141 gccaatgcca gaccgaaaga aattgagcaa ctccccatgg tgaaaggctg tttcaccaat
    10201 gccgggcaat ttcaggtggt gattggcacc aatgtgggtg attactatca agcactgatt
    10261 gcgtcaaccg gacaggcgca ggttgataaa gagcaggtaa aaaaagccgc ccggcagaat
    10321 atgaaatggc atgagcaatt gatctctcat ttcgcggaga tcttcttccc gctactgccc
    10381 gcgttgatta gcggcggttt gatcctcggt tttcgcaatg tgatcggcga tttgcccatg
    10441 agcaacggtc agacgctggc gcaaatgtac ccttccctgc aaacgatcta cgattttctg
    10501 tggttgatcg gtgaagcgat cttcttctac ctgccggtcg gtatttgctg gtcagcggtg
    10561 aaaaaaatgg gcggcacgcc gatccttggt atcgtgcttg gcgtgacact ggtttctccc
    10621 cagctgatga acgcttatct gctcgggcag cagctgccgg aagtgtggga ctttggcatg
    10681 ttcagcatcg ccaaagtagg ctatcaggcg caggtgatcc cggcactgtt agccgggctg
    10741 gcgctgggcg ttattgaaac tcgccttaaa cgcatcgtgc cggattacct ctatctggtg
    10801 gtggtgcccg tctgttcgct gatcctcgcg gtgttcctcg cccatgcgct gattggtccg
    10861 tttggtcgca tgattggcga tggcgttgcc tttgcggtac gtcacctgat gaccggcagc
    10921 tttgctccga ttggcgcagc attgtttggc ttcctgtacg ccccgctggt gatcaccggt
    10981 gtacaccaga ccacgcttgc tattgatttg cagatgattc aaagcatggg tggcacgcca
    11041 gtgtggccgc tgattgcgct gtcgaatatc gctcagggct ccgccgtgat aggcattatc
    11101 atttccagcc gcaagcacaa tgaacgcgag atctccgtgc ctgccgctat ctccgcctgg
    11161 cttggggtca ctgagcctgc aatgtacggc atcaacctga aatatcgctt cccgatgctg
    11221 tgcgcgatga ttggttctgg tctggcagga ttactatgcg gcctgaacgg cgttatggcg
    11281 aatggtatcg gcgtaggggg cctgccggga attctctcga ttcaaccgag ctactggcag
    11341 gtatttgcgc tggcaatggc tatcgccatc atcatcccga ttgtactcac ctcgtttatc
    11401 tatcagcgga aataccgcct gggcacgctg gacattgttt aattttcttc ggggcgcaat
    11461 tgcgccccct cgcatttgca ggaataacgt aatgactaat cttccccact ggtggcaaaa
    11521 cggcgttatc taccagattt atccaaagag ttttcaggac accacgggta gcggcaccgg
    11581 cgatttacgt ggcgttatcc aacgcctgga ctatctgcat aaactgggcg ttgatgccat
    11641 ctggctaacc cccttttatg tctctcccca ggtcgataac ggttacgatg tagcgaacta
    11701 tacggcgatt gatcccacct acggcacgct ggacgatttt gacgaactgg tgacgcaggc
    11761 aaaatcgcgc gggattcgta tcattctcga tatggtgttt aaccatacct ctacccaaca
    11821 tgcctggttt cgcgaggcgc tgaacaaaga aagcccttac cgccagtttt atatctggcg
    11881 cgatggcgaa ccagaaacgc caccgaacaa ctggcgttca aaatttggcg gtagtgcgtg
    11941 gcgctggcat gcggaaagcg aacagtacta tttgcatctc tttgcaccag aacaggcgga
    12001 tctcaactgg gagaatccag cggtacgcgc agagctgaaa aaagtctgtg aattctgggc
    12061 cgatcgtggg gtcgacgggt tgcgcctgga tgtggtgaat ctgatctcca aagacccgcg
    12121 tttccctgaa gacctggatg gcgacgggcg tcgcttctac accgacgggc cacgagcaca
    12181 cgagtttttg cacgagatga accgcgatgt gtttacgcca cgcgggttaa tgaccgtagg
    12241 tgaaatgtcc tccaccagcc ttgagcattg ccagcgatac gcggcactga caggcagtga
    12301 attgtcgatg acctttaatt ttcatcacct gaaggtcgat tatcccggtg gtgaaaaatg
    12361 gacgctggct aaacctgact ttgtggcgtt gaaaacattg ttccgccact ggcaacaagg
    12421 aatgcacaac gtggcatgga atgccttgtt ctggtgtaac cacgatcagc cgcgcattgt
    12481 ttctcgcttt ggtgatgaag gtgaataccg cgtgcctgcg gcaaaaatgc tggcgatggt
    12541 gctgcatggc atgcagggaa cgccgtatat ctaccagggc gaagagattg gcatgaccaa
    12601 cccgcatttc acgcgcatta ctgactatcg cgacgtggag agcctaaata tgtttgccga
    12661 gctgcgcaac gatggtcgtg atgccgacga gttattggca atcctcgcca gtaaatcccg
    12721 tgacaacagc cgcacgccca tgcaatggag caacggcgat aatgccgggt ttacggctgg
    12781 cgaaccgtgg attggcctgg gcgataacta tcaaaaaatc aacgtagaag ccgcgctggc
    12841 cgatgagtcc tcggtgtttt acacctacca aaagttaatc gcactgcgta agcaagaagc
    12901 catcctgaca tggggcaatt accaggatct gctgccaaac agccctgtat tgtggtgcta
    12961 tcgccgtgaa tggaaggggc aaaccttgct ggtcattgcc aaccttagcc gtgagatcca
    13021 accctggcag ccagggcaaa tgcgcggcaa ctggcagctt gtgatgcata actacgaaga
    13081 agcctcacca caaccctgtg ccatgaattt acggcctttt gaggctgtct ggtggttaca
    13141 gaagtaaatc ttccctaagc cccggtaatg ccggtcattc cggggttttg tctgtcagca
    13201 tgttgttttt gttgattttt caaccagcaa attcattaaa aaatttacat aacgctgtag
    13261 cgcccgtcat ccgtacgctc tgctttttac tttgagctac atcaaaaaaa gttaaaacat
    13321 ccttgatgca aagcactata tatagacttt aaaatgcgtc caaacccaat atgttgtatt
    13381 aatcgactat aattgctact acagctcccc acgaaaaagg tgcggcgttg tggataagcg
    13441 gatggcgatt gcggaaagca ccggaaaacg aaacgaaaaa accggaaaac gcctttccca
    13501 atttctgtgg ataacctgtt cttaaaaata tggagcgatc atgacaccgc atgtgatgaa
    13561 acgagacggc tgcaaagtgc cgtttaaatc agagcgcatc aaagaagcga ttctgcgtgc
    13621 agctaaagca gcggaagtcg atgatgccga ttattgcgcc actgttgccg cggttgtcag
    13681 cgagcagatg cagggccgca accaggtgga tatcaatgag atccagaccg cagttgaaaa
    13741 tcagctgatg tcaggtccat acaaacaact ggctcgtgct tacatcgagt accgtcacga
    13801 tcgcgacatt gaacgtgaaa aacgcggtcg cctgaaccag gagatccgtg gtctggtcga
    13861 gcagaccaac tcctcgttac tcaacgaaaa cgccaacaaa gacagcaagg tgattccaac
    13921 ccagcgcgac ctgctggccg ggatcgtggc taaacactat gcacgccagc acctgctgcc
    13981 gcgtgacgtg gtgaaggcac atgagcgtgg cgatattcac tatcacgatc tcgattactc
    14041 gccgtttttc ccgatgttca actgcatgtt gatcgacctg aaaggcatgc tgacccaggg
    14101 ctttaagatg gggaacgccg agattgaacc gccgaagtcg atctctacgg caaccgcggt
    14161 aactgcgcag attattgctc aggtcgccag ccatatttat ggcggcacca ccattaaccg
    14221 tatcgatgaa gtgctggcgc cgtttgttac cgccagctac aacaaacatc gcaagaccgc
    14281 agaagagtgg aacatcccgg acgccgaagg ctacgcaaac tctcgtacca tcaaagagtg
    14341 ctacgatgcc ttccagtcgc tggagtacga agtaaacacc ctgcacaccg ccaacggtca
    14401 gacgccgttt gtaacctttg gttttggtct gggcaccagc tgggaatcgc gcctgattca
    14461 ggaatcgatc ctgcgtaacc gtatcgcagg cctcggtaaa aaccgtaaaa ctgcggtgtt
    14521 cccgaaactg gtgtttgcga ttcgcgatgg tctgaaccat aaaaaaggcg atccgaacta
    14581 cgacatcaaa cagctggcgc tggagtgcgc aagcaagcgc atgtatccgg atatcctgaa
    14641 ctacgatcag gtagtgaaag tcaccggttc gtttaaaacc ccgatgggct gccgcagctt
    14701 cctcggcgtg tgggaaaatg aaaacggcga gcagatccac gatggtcgta acaacctcgg
    14761 cgtgatcagc ctgaacctgc cgcgtattgc tctggaagca aaaggcgatg aagccacctt
    14821 ctggaagctg ctggatgaac gtctggtgct ggcacgtaag gcgctgatga cccgtatcgc
    14881 tcgtctcgaa ggcgtgaaag cgcgcgtcgc cccgatcctc tatatggaag gtgcttgtgg
    14941 cgtacgtctg aatgctgacg atgatgtttc tgaaatcttc aaaaacggtc gtgcgtctat
    15001 ttcgctgggt tacatcggca tccacgaaac cattaacgcg ctgttcggcg gcgagcatgt
    15061 ttacgacaac gagcagcttc gcgcgaaagg tatcgccatt gttgaacgtc tgcgtcaggc
    15121 agtggatcag tggaaagaag aaacgggtta tggtttcagt ctctacagca cgccgagtga
    15181 aaacctgtgc gaccgcttct gccgtctcga tactgctgag tttggcgtgg tgccgggcgt
    15241 gaccgataaa ggttactaca ccaacagttt ccacctcgat gtggagaaga aggtgaaccc
    15301 gtacgacaag atcgactttg aagcgcctta cccgccgctg gcgaacggtg gtttcatttg
    15361 ctacggcgag tatccaaaca ttcagcacaa cctgaaggcg ctggaagatg tctgggatta
    15421 cagctatcag catgtaccgt attacggcac caatacaccg attgatgagt gctacgagtg
    15481 tggctttacc ggtgagttcg agtgcaccag caaaggcttc acttgcccga aatgtggtaa
    15541 ccatgacgcc tcccgtgtgt cggtaactcg ccgcgtgtgc ggatatttag gtagcccgga
    15601 tgcacgtccg tttaacgctg gtaagcagga agaagttaag cgccgcgtta aacatttggg
    15661 gaatgggcag ataggttaat cttcactctg cgctaagcaa atgccggatg cggcgtaaac
    15721 gccttatccg gcctacattt ggcaacggct ccaggcctga taagacgcga cagcgtcgca
    15781 tcaggcattg attaccggat gcagcctaca cgcggactga taagccaggc gaaataatga
    15841 attatcatca gtactatcct gtcgacatcg tcaacggccc cggcactcgc tgcaccctgt
    15901 ttgtctccgg gtgtgttcat gaatgccccg gttgctataa caaaagcaca tggcgggtaa
    15961 attctggtca gccatttacc aaagcaatgg aagaccagat cattaacgat ctgaatgaca
    16021 ctcgtatcaa acgccagggt atttccctct ccggcggcga tccgctgcat ccgcaaaacg
    16081 tgccggatat tctgaaactg gtacaacgca tccgcgccga gtgtccgggt aaagacatct
    16141 gggtgtggac aggctataaa ctcgacgaac tcaacgctac gcaaatgcag gttgttgatt
    16201 tgattaacgt gctggtcgac ggcaagtttg tgcaggattt aaaagaccct tccctgatct
    16261 ggcgcggcag cagcaatcag gtggtgcatc atttgcgatg ataagttccc tgttacctga
    16321 gtgcaggcaa cagggaaatg aggaattaac gatacttctg gtgataggcg ttgcgggtcg
    16381 ttttcagttg ctctgcggcg gcctgcgctt cttttacttt accttcattt gccagcttca
    16441 gcgcgtcgtc aatctgaccg accagaatgt cgaaaccgtg gcggaaatct ttcatttccg
    16501 ggctgtccgg tgatttatct tcgagcttcg gcggcgttgc tttttgcgca tccagcgctg
    16561 cggcgcgcat catcgttaac gcgtctttga cttgcgccgc gttatccgct ttttcgatca
    16621 cttttaaatt gtcgttgagg gtttccatat tgtcttcaag atcagcggca aacgacgcag
    16681 aactgaatac caacgaggag actgcaagaa tagctaacag gcttttacgc attgctcact
    16741 tcctttttta tttacacttc atccttcaca ctgcctcatc gacggctacg ctcattcgcc
    16801 cgggtcacgt actgcgagta cgctccccgg ccctcattca ctttccgtct cgatgcagtg
    16861 cgaatgattt tgtgtaattt tttagtttat agcgcggcag gtcgcgccag ttttttactg
    16921 cccggcgatt ttcatctccg gcaacagcac agaaccacac tgtatattac tgcgtgtttc
    16981 aatatcgtta ccgacggtga caatattgcg ccacatatct tttaaattac ctgcgatggt
    17041 gatttcacta acgggatact gaatttcgcc attctctacc cagaagcccg ctgcaccacg
    17101 ggaataatca ccggtaatgg cactcacgcc ctggcccatc agctccgtca ccaccagccc
    17161 ggtgcccatc tctttgagca tttgttcgaa gcttagccct tgtccggcaa tccgccagtt
    17221 gtgaataccg cccgcatgtc cggtgctttt cagccccagt ttccgcgccg agtagctggt
    17281 cagcagccac tgagtcagga tgccatcttt aataatatcg cgacgctcgg tgcgcacacc
    17341 ttcgctgtcg aatggcgtcg acgccagccc tttcagcaga tgcggatgct cttcaatggt
    17401 cagccagtcc ggcagaattt gtttacccag cgaatccagc agaaaggtag atttacggta
    17461 aaccgagcca cccgctatcg cccccaccag atggccaaaa agcccggttg ccacttcatt
    17521 ggcaaaaatg actggcgctt tcatggtgga gagtttacgc ggtgacaggc gcgataaagt
    17581 acggcgagca cagtcggccc caacccactc tggcgtttgc agatcgctca tcgcacgacc
    17641 aatggtgtag gcgtaatcac gctccatatc gccattttct tcggcaatta cacagctgga
    17701 gagtgaatga cgcgtagagc agtaaccctg caacatgccg tggctgttgc caaaaacttt
    17761 gacaccgtag tggctgttaa aactgccgcc ttcggtattg gtaatgcgtt tgtccgcctg
    17821 caatgccgcc tgttctgcgc gggccgccag ttcaatggct tcatccgggg aaacttccgc
    17881 agggtggaac aagtcgagat ctggtgcgtc aaaggccagc agctctttgt ctgccacgcc
    17941 ggcacaggga tctggcgagg tataacgggc aatatccagc gccgcctgta cggtgcgggc
    18001 aatggcctgc gggcttaaat cggtggatga tgcgctacct ttgcggttct ggtgataaac
    18061 agtgatcccc agcgcgccat cgctattgaa ttcaacattc tccacttcac cataacgcgt
    18121 gcttacgcta atgccggtgg tcttgctgac ggcaacttcc gcaccgtccg atttgcctga
    18181 ggccaactcc agcgcagtgg aaactgcttc ttccagaatc ttgcgctgcg cttcaacttg
    18241 agagattact ttcattgcaa gtgccataat gtagagagtt tctctgaagt ctaacagaga
    18301 accgtttttc agtgcgcatc ttaactggta acattagcct ctttttttaa ggagcctgag
    18361 atgactaagc agcccgaaga ctggctcgac gacgttcccg gtgatgacat cgaagacgaa
    18421 gacgatgaaa ttatctgggt cagtaaaagt gaaattaaac gtgatgccga ggagctaaaa
    18481 cgccttggcg cggaaattgt tgatctgggg aaaaacgcgc tggataagat cccgttagat
    18541 gcggatctgc gcgcggctat tgagctcgcc cagcgtatta agatggaagg tcgccgccgc
    18601 cagttgcagc tcatcggtaa gatgctgcgc cagcgcgacg tagagcctat tcgtcaggcg
    18661 ctggataagc tgaaaaaccg tcacaaccag caggtggtgc tgttccataa actggaaaac
    18721 ctgcgcgatc gtctgatcga tcaaggtgat gacgccatcg ctgaagtatt gaatctgtgg
    18781 cccgatgccg atcgtcagca gctgcgtact ctgatccgta acgcgaagaa agagaaagaa
    18841 gggaataagc cgccgaaatc cgcacgccag attttccagt atctgcgcga actggcggag
    18901 aatgaagggt aattttcgtt gttgatgtga gtatggtttg ccggatgcgg tgcacctgta
    18961 ggccggataa gacgcatcaa gcgtcgcatc aggcactatg ctcaaatgcc ggatgcggcg
    19021 taaacgcctt atccggcaat gctatctcag gctgaggccg aattactgcg cagcttccgc
    19081 cttcttcgcc agaccatcca gcagtttctg atggatccca ccaaaaccgc cgttgctcat
    19141 gaccagaata tggtcgccag gctgagcggt tttcaccacc atatctgcca gcgtatccac
    19201 atcgccactc cagtgtgcag gctgaacgca ggcttctgcc acttctgcca cctgccacgg
    19261 aatatgcgcc ggttgcagca ggaagacttc atcggcacga cctaatgaag gtgccagatc
    19321 gtctttgcag atccccattt tcatggtatt cgagcgcggc tccagcacag caataatgcg
    19381 cgccgtacca ccaactttgc cacgcagcgc cgcaagcgtt gccagaattg ccgtcgggtg
    19441 atgggcaaaa tcgtcatata ccgtcacgcc attcgcttca ccacgcaact ccagacggcg
    19501 acgagcgtta ataaacgaac ccagcgcgtt agcggcatct gcaggcgcta caccaacatg
    19561 gcgagccgcc gcaatcgcca tcaggccatt gtgcatatta tgttcgccca ccagcgacca
    19621 tttcacttcg cccacttttt cgccatccag caagacttcc cattcagaag cgtcagtggt
    19681 cagctttttc gcctgccagt gaccctgctc acccaccagc tcctgctcgc tccagcagcc
    19741 catcgccatg gtctgtttca ggttgatgtc attttccggc cagataatac ggccctgccc
    19801 cggaacgata cgcaccagat ggtggaactg tttctggatc gctttcaggt cgtcaaagat
    19861 atcggcgtga tcgaactcaa ggttgttgag gatcagcgta cgcgggcagt aatggacaaa
    19921 tttagagcgt ttgtcgaaga aggcgcagtc atactcatcc gcttcgataa caaagaagtc
    19981 gctttcgccc agacgcgccg aaacctcaaa gttccccggc acaccgccga tcacaaatcc
    20041 cggtttgtaa ccgcactgtt ccagaatcca ggtcgccatt cccgcggtgg tagttttgcc
    20101 gtgtgtaccg gcaacggcca gcacccagcg gtcgcgcagc acaaaatcgt gcagccactg
    20161 tggacctgac atataaggga tgtttttttc cagtaccgct tccacacacg gatttccacg
    20221 ggtcatggcg ttgccaataa tcaccagatc cggctgcgga tcgagctggc tggcatcgta
    20281 accctgaatc agctcaatgc cttgcttctc aagtaaggtg ctcatcggcg gatacacatt
    20341 ggcgtccgaa cccgttactt catggcctaa ctggcgcgcc agcatcgcca gaccgcccat
    20401 aaatgtgcca caaattccta aaatatgaat gcgcatacgt cactatcctt ttttaatctg
    20461 ccgctcattt tacgcatatg tcgggcaagt gagaaacgca tttcaggata atccgcaatt
    20521 tgctggcgcg attcacctga gcgcaacatt gagattattt gttaagattg ttgcggtcgc
    20581 tttactccat aaacattgca gggaaagttt tatgaaaacg ttaggtgaat ttattgtcga
    20641 aaagcagcac gagttttctc atgctaccgg tgagctcact gctttgctgt cggcaataaa
    20701 actgggcgcc aagattatcc atcgcgatat caacaaagca ggactggttg atatcctggg
    20761 tgccagcggt gctgagaacg tgcagggcga ggttcagcag aaactcgact tgttcgctaa
    20821 tgaaaaactg aaagccgcac tgaaagcacg cgatatcgtt gcgggcattg cctctgaaga
    20881 agaagatgag attgtcgtct ttgaaggctg tgaacacgca aaatacgtgg tgctgatgga
    20941 ccccctggat ggctcgtcca acatcgatgt taacgtctct gtcggtacca ttttctccat
    21001 ctaccgccgc gttacgcctg ttggcacgcc ggtaacggaa gaagatttcc tccagcctgg
    21061 taacaaacag gttgcggcag gttacgtggt atacggctcc tctaccatgc tggtttacac
    21121 caccggatgc ggtgttcacg cctttactta cgatccttcg ctgggcgttt tctgcctgtg
    21181 ccaggaacgg atgcgcttcc cggagaaagg caaaacctac tccatcaacg aaggaaacta
    21241 cattaagttt ccgaacgggg tgaagaagta cattaaattc tgccaggaag aagataaatc
    21301 caccaaccgc ccgtatacct cacgttatat cggttcactg gtcgcggatt tccaccgtaa
    21361 cctgctgaaa ggcggtattt atctctaccc aagcaccgcc agccacccgg acggcaaact
    21421 gcgtttgctg tatgagtgca acccgatggc attcctggcg gaacaagcgg gcggtaaagc
    21481 gagcgatggc aaagagcgta ttctggatat catcccggaa actctgcacc agcgccgttc
    21541 gttctttgtc ggcaacgacc atatggttga agatgtcgaa cgctttatcc gtgaattccc
    21601 ggacgcgtaa tctcctgcgc ctctccacac ggagaggcgt ttttatcgct gcgcaatatt
    21661 gactcttgtc actggcgaac tctgacgatt ctcccacagc accgttaatc cacgctgtaa
    21721 ggcaataaaa atgaacagca aaatgccgat ggcgattttc gtccaccagg aactcagcgt
    21781 accgtcaaag ttgatataag tctgaattag cccctgaatc gccacgccaa aaagcgtccc
    21841 taataccgtt ccaacgccac cgctcaaaag cgtaccgcca attaccactg aggcgatagc
    21901 atccagttcc acacctacgc ccgccagcgc atatccggcc tgggtataaa tcgagaagac
    21961 aatccccgcc agcgttgcca gtccggtgga gagcatataa atgcgaatag tggtgctgcg
    22021 ggtagaaatc cccatcaggt ttgccgacgt tgcgttgccg ccaatggcgt atacctgatt
    22081 accaaaacgg gtacgatgcg cgaggaatat gccgataacc accaccgcca gcatcagcag
    22141 tcccatcgca cttaagcgac cgccgccagg gattttccac gcaaggcttg agagcgtgtc
    22201 ataaatggga tggtttatcg ggatcgactc ttctgaaacg agatagctga cgccgcgcaa
    22261 aaagaacatt cctgccaggg taatgataaa tgccgggatc ttcagggcgt cgatcaaaag
    22321 ccccataaat gcgccaaagg cacagcccat caccagcact agcggaaacg ccagcagcgg
    22381 cgagaggccg aaatcgccaa tcacttttgc cagaaacacg ccagtaaagg cgatcaccga
    22441 accgacggag agatcgatac cgccagagag gatcacaaag gtcatgccaa cggcgattat
    22501 cccaagaaag gcgttatcgg tcaggatatt gcagatcact cttgtggaag caaaaccggg
    22561 aaactgggtc aggcagtaga gatagcccag cacaaagacg ccgatggtga tcatcagcgg
    22621 caagttacgt tttatcatgg ctacgtactc ctttaatcag actgataaag cgttgcgact
    22681 gaacaatcag cacacaaagc accaccaccg cttttacgac ctggttcatc tccggcggaa
    22741 agccagaaag taaaattccg gtgttcatcc cctgaataat cagcgccccc acgactgaga
    22801 gcagcaggtt aaagcgcccg cccatcagcg atccgccgcc aatcaccacc gcaagaatgg
    22861 cgtccagctc cagccataac ccggcgttat tggcatcggc accgcgaata tccgccgcca
    22921 cgataatgcc cgcaatcgcc gcacacagcc cgctcaacac gtaggtgagc atgacgatga
    22981 tccgcgtgtt taccccggca ttttttgccg cccgaatgtt gataccaacg gcttcgataa
    23041 acatccccag cgccgttttg cgggtcaaca gccagaacag gagaagcgtc agcaccgcga
    23101 taatgaccgg cgttggcagg aacaacagcg atccgctgcc aaaccatgag agatccggcg
    23161 agttaaacgt gacgatctgc ccggcggtga tcagttgcgc cacgccgcgc ccggcgacca
    23221 tcaggatcag ggtggcgaca aacggctgaa ttttgaggat cgctaccagt atgccgttcc
    23281 acaatcccgc caggatgccg gtgcccaggg cgcttaacaa aacaatcggc aggctgaatc
    23341 ccgcgaccgt catcgcagcc gttgtggctc cggcgatagc catcaccgcc cctacggaga
    23401 gatcaatccc accggtggcg atcaccagcg tcataccaat cgccagtaac gcaacgggag
    23461 ctgcacggtt aagaatgtcg atagggctac cgaacaaacg cccatcctga agcaccacct
    23521 gccagaaatg cggggctacc aggctatcga ccagcagcac cagcaatagc gccgccagct
    23581 gcggcattcc cgttggccag cgaaagcgcc ttttcggcgg cgtagtgtcc gggagagatt
    23641 gaggcatcac actgttctcc ttacgccgca atggcgttca tgatcgccgg aacggaaagc
    23701 tctgccagcg ggatctccgc cacctgtttg cgatcgcgca tgataatcac ccgatcggca
    23761 tagcccacca gctcttccag ttcggaggag atcaccaaca gcgccagacc gtcagcacac
    23821 agcgtttcaa tcaggcggat gatctcggcg tgggcaccaa catcaatgcc gcgggttggc
    23881 tcgtcgagga tcagaaattg cggtcgggtc agtagccaac gtgaaagcaa cactttttgc
    23941 tgattgccgc cagagagaaa ttcaatcggt tgttcagttg aaggtgtgcg aatgccaagc
    24001 tggcggataa agcgttcggc aatctcttgc tgctctttgc gggaaatggg tcgtagccag
    24061 ccgcgctggg cctggagagc gaggatgata ttttcccgta ccgaggcggc ggcgatgatg
    24121 ccatcggttt tcctgtcttc cgggcagaag ccgatgccca gtaccgaagc ctgatgtggc
    24181 gatcgcaggt tttgctgttt gcctttgatc aacgccgtgc cgctgtcagc aggtttgata
    24241 ccgaagatca cttcggcggt ttcggtacgt ccggatccca gcaagcctgc caggccgacg
    24301 atctcgccgg ggcgtacttc gagatcaaac ggtgcgatcg ttcctttttt gccgtaattt
    24361 ttgaacgcgg caacgggttt gtcgctcaac aatgttcgcc cggcacgctg tagcgcgtgg
    24421 gtatccagct cgcgccccag catcattttt accagttcga tctgcggtag ctcgcgcgtt
    24481 tcccgacagc ctacgaaact gccgttgcgt aagacggtga tccgatcgct gacctgatag
    24541 acctgatcga gaaagtgagt gacaaagatc aggctgacgc cgcgatcgcg caactgacgc
    24601 atcaggccaa acagtaactc tacttcctgg gtgtcgaggc tggcggtggg ttcatcgagg
    24661 atcagcactt tggcggagag atcgatagcc cggcaaatgg cgacgatttg ctgcatcgcc
    24721 accgaaaaac ggttaagcgg ttcacgcacg tcgagggaga aaccgtaaga tgtcatcagt
    24781 tcggtggcac gcttttccat ctctttgcgg cgtagaaagc cgaaacgttt gggttcgcga
    24841 cctatgaata gattatcagc gaccgacata ttgggtagca ggttgacttc ctgataaacg
    24901 gttccgatgc cgagttgttg tgcatgggcg gtatttttcg gtgagatagc ctggccttcc
    24961 agccagatgg tgccgcgatc ggcgtggtat acaccggtta atgctttgat tagcgtcgat
    25021 tttcccgccc cgttttcacc gagcagcgcc atgatttcgc cacggcgcag gctgaaatca
    25081 acgttgtcta acgctttgac gccggggaaa aatttactta atccttcggt gcggaggatt
    25141 tcctggtgtt ggtcggtcgt catggtttcc cccttagcca cgctctaaaa gtctgggaat
    25201 agatcgtgcc tgatgttaag agctgcgccg tttgtccccc tcaccctaac cctctccccg
    25261 aaggggcgag gggacagatt gtgcacggtg ttaagtccgg tgttgcacca ctgccggatg
    25321 cggcgtgaac gccttatccg gcctacacag agcaccgaac tcgtaggcat gataagacgc
    25381 ggtaagcgtc gcatcaggct tcagcgcacc accgccggat gcggcgcgag cgccttatcc
    25441 ggcctacgaa tcggcatcag gcattgatac cctcaatacc ccatattttt cttcttctct
    25501 aactcttctt ttgcggtatc aggcagataa agggtggatt tggtcagcgt tagcttttca
    25561 ggcatggtgc cgtctttttt gtatttctcc agcgcgtcga aggcgggacc tgccatattc
    25621 ggcgtcagtt caacactggc gttcgcttcg ccatcaatca tcgctttgta gatatccggt
    25681 acgccatcaa tggaacccgt gaggatatct ttacccggct tcaggcccgc ttctttaatt
    25741 gcctgaattg caccaatcac catgtcatcg ttatgggcgt aaaccatgca gatgtttttg
    25801 ccgttgtttt ccgctttgat aaagctctcc atgacttctt tgcctttact gcgggtgaag
    25861 tcacctgact gcgagcggat aattttgata tttggcgcat tcttaatggc ttcggcaaag
    25921 cctttcttac ggtcaatggc gacgctggcc ccaacggtgc cctgcagctc caccacgttg
    25981 catggtttgc cattcacttc ttttaccagc cagtcaccaa tcaacttgcc ttcgaggatg
    26041 ttgtcggcgg tgacagtggt catatagaga gatttgtctt tcacatcgat ggaacggtca
    26101 agcaagaaga ccgggatttc ggcatctttc gcctctttta ataccggctc ccaacccgtc
    26161 gctaccactg gagcgataaa gatcgcatcc accccttgtg caacgaagga acgtaccgct
    26221 ttaatctggt tttcctgctt ttgttgacca tcggcaattt tcaacgtaat tccgcgtttt
    26281 tcggcctcac ttttcgccac attggtttca gcggcacgcc agcctgattc cgatccgacc
    26341 tgcgaaaatc ctacggttaa cggagcggcc aacgccatag acgacatggc tgccgagact
    26401 gcagagacta caagtaagcg tttccacata gggttgtcct cgtcggggta attattattg
    26461 gtgaaaagat gttcgcgaaa aaactataga caattcgtta tgtaacggat tgcgttacat
    26521 cacacttcaa aaaagtaaat aaaacgttaa tcacaagttt gtaatcgctt tcatctcact
    26581 atgaaaaatg cggctacggt tatggatttt cctgctctgc ataccgtctt aaaactggcg
    26641 aaaaaggaaa atgaagacga aaacaagcga agacattcgg cgcgagttgg ctataatact
    26701 cggcacttgt ttgccacata tttttaaagg aaacagacat gagcttactc aacgtccctg
    26761 cgggtaaaga tctgccggaa gacatctacg tggttattga gatcccggct aacgcagatc
    26821 cgatcaaata cgaaatcgac aaagagagcg gcgcactgtt cgttgaccgc ttcatgtcca
    26881 ccgcgatgtt ctatccgtgc aactacggtt acatcaacca caccctgtct ctggacggtg
    26941 acccggttga cgtactggtc ccgactccgt acccgctgca gccgggttct gtgatccgtt
    27001 gccgtccggt tggcgttctg aaaatgaccg acgaagccgg tgaagatgcg aaactgattg
    27061 ctgttccgca caccaagctg agcaaagaat acgatcacat taaagacgtt aacgatctgc
    27121 cagagctgct gaaagcgcag atcgctcact tcttcgagca ctacaaagac ctcgaaaaag
    27181 gcaagtgggt gaaagttgaa ggttgggaaa acgcagaagc cgctaaagct gaaatcgttg
    27241 cctccttcga gcgcgcaaag aataaataag ttcttctggc gcaataaccc tgaacaccgg
    27301 gcttcggtta gtaagggttt ttttatgccc gcgataaata aacacacctt attccaccac
    27361 cgcctgcaag cgtaataacg cctcttccac cacctcatcc gcagccagac caatacgctt
    27421 tgccagccgg gcgcgtagat ccatcatccg cacctgattc accagcacca cgccgtgcac
    27481 atcgccttct tcgcaatgta aaggaacgct aaatccggca taacgggcaa aatttccgcc
    27541 ctgcgtaatg ggggccacca gcgtcattcc cagttgatta aaggcttgaa cggagagcac
    27601 aagcgcaggt cgaccagcac cttgctgttc atggccgctt gctggatcaa agccaaccag
    27661 cacaatgtct ccccgttcaa attcactttt ctttaccata tttcgtcacc cgcaggggtg
    27721 gatttacccc agacatcctg ctcgctaagt tccgcggcgt tcatgtcaca ctgtgccagc
    27781 agttcatcaa gcgagtagcg cctggagatg ggtgtcagaa tcagttgatt gttgctcact
    27841 tgcgcctcca cgctctgccc cggctgtaag ttaagttctt tcattacgat attgggaatg
    27901 accatacctg cactgttccc ccatcttttt atggtaatac gcatctgcac ctccttaagt
    27961 tatacaaagt ataactcacg gtgctacagg aaacgtttta cccctgcgca tttgtgaacg
    28021 ccagcagagt tttttatgcg ttaacagata tggaaagcgg ctcggagaga aaagcaaagg
    28081 gtgagggaat tacaaaacag aatgcgagtc gtctcgcaaa aaacaacgcc acccgaaggt
    28141 ggcgtatgca tatggttact tatccctgtc taaccagtcg ccgctttcaa ttagctttaa
    28201 tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa
    28261 ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt
    28321 gtcaatacga taaacttcac cgtgtactgt tccgttcccc ggaactgcgc ctggatagtg
    28381 gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt
    28441 catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc
    28501 taaaactcga actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga
    28561 tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc
    28621 acgccgtatt tcacttgcag acctggcaat acatagccgc tgactactac ctgggaggag
    28681 tcgcctactc cctgggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta
    28741 cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca
    28801 ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt
    28861 tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg
    28921 cgaactccgg cgattacgtc gtcttctgta gcatccgggt tacgaatagc ttcaatatta
    28981 agatacggtt gatccggcgg accagagaac agtaactcac ccttacgcac aatcagatcc
    29041 tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct
    29101 tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca
    29161 atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt
    29221 ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact
    29281 gcgctttccg gcagatcgtg caccacgatg cgcgcccacg ggacatccac gcgaccatcg
    29341 agggtaaaca ggtttggtgt agcctcgaat acaacatctg gcgatacatc cattcgtacc
    29401 atcggcggca cggtgatccg cactttactg cctttcgccg ttactcgtgc ccgccagttt
    29461 tcaatttggc tccagtcggc atcaccgttc aggtagattt caccctgctg ggtccgtact
    29521 gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg caagctggct cggctgcata
    29581 tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac
    29641 tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt
    29701 tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg
    29761 ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg
    29821 ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc
    29881 agattcagtg tctgaaacgc caccggcagc gccgcatcgt tgacggtttg cgttacctgc
    29941 acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttcgt ggtgtcccag
    30001 gcaacatccg ctttacccgt gaagataccg ctggcctgag tggtttgcgg cataaacggt
    30061 ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcccccgca
    30121 tcgatagttt gcggcacaca cagctccgca ttcgggttaa gccaacagtg tggcccgatg
    30181 ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg
    30241 ccgaccggcg tctggaagcg ggtattgcta agaattccct tccagcgttc ttctttgcga
    30301 tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt
    30361 agctcgtgct gcttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc
    30421 ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta
    30481 atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg
    30541 gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc
    30601 gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca
    30661 agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg ccccagttcc
    30721 agatgaagcc ctgggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg
    30781 ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag
    30841 gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactcc
    30901 gggaactctt tggcggtgtt aatgccatta agcgttagct caccgcgcca actaatggcc
    30961 tgttgccagt cgagcaacgc cttgagttca gttttgcctt ccagcgccgc aacggtgagt
    31021 ttgtcgagat tgacctgctg ttcattacct ttcgcgtcga gggtaatagt cgcgggcggg
    31081 atctcctgtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta
    31141 agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaagc
    31201 tgtttgctgt tcacttccac gttgagcggc aatccagctt ccgccagtcg cgtctgggcg
    31261 cgtaaatcca tatccaccgg accggaaagg ttaacgccaa tctccagctg ttcgcgcagc
    31321 gcgccgccca ctttcagctt cactttttca cctttcaacg gctccacgtt tagtgtgctg
    31381 ttgagggtga tatccaccgg ccagttgtct gacagctgtg ccgtgccgct ggcgttgacg
    31441 atcccttgat tggaatcgat atccagggcg tccagtttag tattaccgtc gatgctgctt
    31501 actttcagca gcatggtgtt cacggtgatg tccgtgtcgc ccgtcacgcg cagctgctcg
    31561 cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat
    31621 accggacgag aaaagagatc tttcaacgtt tcgccgagcg gcttttcatc cggctgcgga
    31681 ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccgccacttt cggcagggca
    31741 atcaacaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcagg
    31801 ccggaggtga agtccatcac cgataccgtg gtgtcatcaa tcttgatgtt gacgttatcc
    31861 agcgccaccc gtgtcagggt gatgggatac ggcgtggaga gatccagcgg accgctatct
    31921 tcctcttctt caacctgttc agaagggggc atttttttac tgtcgatgtt gacctgaatg
    31981 tctttcagcg ccaggtcatt aatacaaacg ctactgttcc acaggcactc aagaccgacc
    32041 gccagatgca gattacccgc ttttaccgcc acgcctggct gctcataacg aacgtcagac
    32101 aaggtgagat cgcgccagcc gccggtgacc ttgccaatat ccagtcctgg cacccagcga
    32161 tccgccgctt taaatacaag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat
    32221 cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata
    32281 attctggccc cagaccgatg taaaactgta acccgtgctc gtctttatcc gcgaccggta
    32341 cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgtacgccg accccggtac
    32401 cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg
    32461 cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt atcagcttcg
    32521 aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat ttgtagccgc
    32581 gaatactgcg atcgcccccg gcgaagaaac gcagatccgg cggtactttg tcgaaatcac
    32641 ccgtttcaat ccagcccagc gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc
    32701 ggatccagac gttctgcgcc tggaaaacgg agaaatcgac atctgagccc caggccgtgt
    32761 tggagtagtc gatagagtaa cgttgcgagt cgccccaggt tggcatcagg ccaccacgag
    32821 aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac
    32881 cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc acgctgccag ccgctggaga
    32941 gatcccagta gcgagaagcc accagcgtag tggagtcaga ttcggtatcg ttcaggtcag
    33001 tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc agcagcggca
    33061 ttttatagct gaagtcgagg gtctgttccg gcgcggaaat actggtactg gtggtcagac
    33121 tgtgaccata agagttcatc cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt
    33181 ccgtagagta accgaccccg gtttcgatgg tgttttcagt tcgcggcgaa accacgcccg
    33241 tcaagggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc accaccgagt
    33301 taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcgtact
    33361 catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt
    33421 caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg
    33481 ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac
    33541 ctttacgcaa cgcaatgctg gttaaggact ttttgaaatt ttcataatcg ccctggttca
    33601 gcaccgtgcc aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg
    33661 cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacacct ggcgtgactt
    33721 tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg
    33781 gctggtaata acccagcgct ttcagacctt cacggatggc atcatcgacg cgtgcgcgaa
    33841 agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgca cgaacgttct
    33901 tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc
    33961 cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg
    34021 aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata
    34081 tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag acctattcac
    34141 ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg accgattttc
    34201 gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc
    34261 tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa
    34321 tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg
    34381 tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggag
    34441 gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag
    34501 cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt
    34561 gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc
    34621 gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc agtctggaac
    34681 gttttcaggc ggcgatgctt gccgccgatg atgatcgtca catcaccacg gaaatcgcta
    34741 acgccacacc gttttattat gccgaagatg accaccagca atatctgcat aaaaacccgt
    34801 atggttactg tggaattggc ggaattggcg tctgtctgcc accggaagca tagcgttacg
    34861 ggtacaaatg tagattgttg ataaagtgcg ctttatttat gccagatgcg gcgtaaacgc
    34921 cttaccaggc ctacaaaatc gtccaaattc aatatattgc aaggactgcg taggcctgat
    34981 tggcatagcg catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg
    35041 actttacagt accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc
    35101 ggctttcaat gtgtatttca cacaaattaa tcaacttccc cttccgagga tctggcctga
    35161 aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgtttgat cgctgtaagt
    35221 gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg
    35281 ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc
    35341 atgttcttta ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc
    35401 ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagaa
    35461 ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg
    35521 tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt
    35581 atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac
    35641 ggactggcga acataatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc
    35701 acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcaggcgt gttacgtaaa
    35761 caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca
    35821 atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag
    35881 aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac
    35941 atcatcggtt atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg
    36001 gcactgaaca gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt
    36061 tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag
    36121 tacgcgctgg tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat
    36181 ctggtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt
    36241 gacggcggca ccccaattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag
    36301 tcgggcaact acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa
    36361 cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac
    36421 cgcatcgacc agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa
    36481 ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct
    36541 cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca
    36601 gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt
    36661 ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aacgtccttg atgtataacc
    36721 gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa
    36781 ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta
    36841 ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg
    36901 cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc
    36961 ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat
    37021 cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg
    37081 gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac
    37141 ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaaccggtc agcgagtgcc
    37201 gccgattggc atcaccgatc gtggcgagtt ggtgcttgat aaagatcagt ttagctacaa
    37261 aacctggaac agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg
    37321 cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc
    37381 gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcaattc cgggttccgg
    37441 catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat
    37501 tgttgatagc aatggcgtcg cacgcggtgc ctggcagctg gatgaaaaaa gttccgctgt
    37561 agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc ttactcagga
    37621 agaggtgcag caagtgatgg acctgctgca taaattaatt aataaataga cactctgaac
    37681 ccaggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg ccagtcgtga
    37741 acgtgcgctc cggcagctgc ggccacagca tgtccagcgg cggtgtccca aatattcgtt
    37801 ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca ccaggcagaa tttcagcgaa
    37861 gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt cagctccgca
    37921 tctgcatggg aacggctgat aaccaccagc ggcgggcgcg catcgcggac ctgaatctgc
    37981 ttgcgcacac cgcactcttc tttccaggcc ttgccttctg ccgcgctgta cattacgttc
    38041 attaccggcg catacaccac acctaaaatc ggtttgccat ggtcaatgag cgcaatgtta
    38101 acggtgaatt cgccattgcg tttaataaac tctttagtac catccagcgg gtctaccagc
    38161 cagtaacgct gccagtgctg acggacttcc caaccgggag gatcttcttc agaaaggacc
    38221 ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa cggtgtgtgc ggcaatatcc
    38281 gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt cgtcccgtcg
    38341 tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg atctaacatt
    38401 tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc ctatcgttaa
    38461 tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg gcattcttca
    38521 ctgttctata agtaagacgt ttattctccc ttttctttcg tattcccgat aataaaagga
    38581 tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc gccagtgtga
    38641 atgcagcgac ggtcgatctg cgtatcatgg aaaccactga tctgcatagc aacatgatgg
    38701 atttcgatta ttacaaagac accgccacgg aaaagttcgg actggtacgc accgcaagcc
    38761 tgattaacga tgcccgcaat gaagtcaaaa atagcgtgct ggtggataac ggcgacttga
    38821 tccagggaag tccgctggcc gattacatgt cggcgaaagg attaaaagcc ggagatattc
    38881 acccggtcta taaggcatta aatacgctgg actataccgt cggcacgctt ggcaaccacg
    38941 agtttaacta cggtctggat tacctgaaaa atgcgttggc aggagcgaaa ttcccttatg
    39001 taaatgccaa cgtcattgac gcaaaaacca aacagcccat gtttacaccg tatttaatta
    39061 aagacaccga agtggtcgat aaagacggaa aaaaacagac gttaaagatt ggctatattg
    39121 gcgtcgtgcc gccgcagatt atggggtggg ataaagccaa tttgtccggc aaagtcaccg
    39181 ttaacgatat aacagaaact gtgcgcaaat acgtgcctga aatgcgtgag aaaggtgccg
    39241 atctcgttgt cgttctggcg cattccgggc tgtctgccga tccgtataaa gtgatggcgg
    39301 aaaactcagt ttattatctc agtgaaattc cgggtgttga cgccattatg tttggccatg
    39361 ctcacgccgt tttcccaagt aaagattttg ctgatatcga aggagctgat atcgcgaaag
    39421 gcacgctgaa tggtgttccg gcggtaatgc caggcatgtg gggcgatcat cttggtgtgg
    39481 tcgacttaca actcagtaat gacagcggta aatggcaggt gacgcaggcg aaagcggaag
    39541 cacgaccgat ttacgacatc gctaataaaa aatccctcgc ggcggaagac agcaagctgg
    39601 tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag ccaatcggta
    39661 aatctgccga caatatgtat agctatctgg cactggtgca ggacgatccg accgtgcagg
    39721 tggtgaacaa cgcgcaaaaa gcgtatgtcg agcattacat tcagagcgat ccggatctgg
    39781 caaaactgcc agtgctttca gctgccgcac cgtttaaagt tggtggtcgc aaaaatgacc
    39841 cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc gccgatcttt
    39901 atctctaccc caatacgctg attgtggtga aagccagcgg taaagaggtg aaagagtggc
    39961 tggagtgctc cgccggacag tttaaccaga ttgatcctaa cagcacgaaa ccacagtcac
    40021 tcatcaactg ggatggtttc cgcacttata actttgatgt tattgatggt gtgaattatc
    40081 agattgatgt tacccaaccc gcccgttatg acggcgagtg ccagatgatt aatgccaatg
    40141 cggaaaggat taagaacctg acctttaatg gcaagccgat tgatccgaac gctatgttcc
    40201 tcgttgccac caataactat cgcgcttacg gcggcaaatt tgccgggacg ggcgacagcc
    40261 atatcgcttt tgcttcaccg gatgagaacc gctcggttct ggcagcgtgg attgctgatg
    40321 agtcgaaacg tgcgggggaa attcacccgg cggcagataa caactggcgt ttagcaccga
    40381 tagctggcga taagaaactg gatatccgtt tcgaaacttc tccgtcagat aaagccgcag
    40441 cgtttattaa agagaaaggg caatatccga tgaataaagt cgcgaccgat gatatcgggt
    40501 ttgcgattta tcaggtggat ttgagtaagt aaaacacttc tttttcggcc tataaatcat
    40561 caaccgcatc cggcatttat tggcggatgc gatgctggcg catcttatcc gccctacaag
    40621 ccatgcaccg tagaccagat aagctcagcg catccggcag ttatgccgca cgttcatccc
    40681 gcaccgccaa cacctcgggc aaattcaact caatccagtc cgccagtgca gcaacctttt
    40741 cgctcacctg ctcgcccagc ggcgtgaggc tgtattccac atgcggcggc accaccggat
    40801 acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg taacgactgc gcaagcatct
    40861 tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta
    40921 gcgccaccag aatcaatacc ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg
    40981 ggcactgttc cgcaaagaga ttaccctctt tcagttgttg cgacaggcta acctgactca
    41041 tttcatactt acctttttgt acgtacttac taaaagtaag tttaggtgtt agcgtattta
    41101 aacacaagac aaaacgatgg agacttccca tgatcgctat tactggtgcc actggccaac
    41161 ttggtcacta tgttattgaa tccttgatga aaacggttcc tgccagccaa atagtggcta
    41221 tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca aggcattacc gtgcgtcagg
    41281 ctgactacgg cgatgaagcc gcactgacat ctgcacttca gggagtggaa aaactactgc
    41341 tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg
    41401 caaaggcggc tggcgtgaaa tttatcgctt ataccagcct gctacatgca gatacctccc
    41461 cgctcggcct cgccgatgag cacatcgaga cggagaaaat gctggctgat tctggcatcg
    41521 tttacaccct gctgcgcaac ggctggtaca ccgaaaacta cctcgccagc gccccggcag
    41581 cactggaaca cggcgtattt atcggtgcgg cgggcgatgg caaaatcgcc tcagcaacgc
    41641 gggcagatta tgcggcagct gcggcacgcg tgattagcga agccggtcac gaaggcaagg
    41701 tttacgaact ggcgggcgat agtgcctgga cgttgacaca gttagcggca gagctgacca
    41761 aacagagcgg aaaatccgtt acctatcaaa atctgagcga agccgatttc gccgcggcac
    41821 tgaaaagcgt cggactgccc gacggactgg cggatatgct ggcggattct gacgttggcg
    41881 catcgaaagg tggtctgttt gataacagca aaacgcttag caaattgatt ggccgcccaa
    41941 cgacaacgtt agccgaaagc gtaagccatc tttttaatgt taataactag ttaattaaag
    42001 tggcatcctc ccgcatcctc tctgataatg acgggatgcc gggagcaatc atgtctgctt
    42061 cctgaacttt cttccgacag accaatggat gccagtaatg attagcggcg tgctgtacgc
    42121 cctgttagca gggttgatgt gggggcttat ttttgtcggg ccgttgatcg taccggaata
    42181 cccggcgatg ttgcagtcga tggggcgtta tctggcgtta gggttaattg cgctgcccat
    42241 tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt cgggactggc ttaccgcctt
    42301 gatgctcact atgatgggca acctcattta ttacttctgc cttgccagtg ccattcaacg
    42361 tactggcgcg cctgtttcca cgatgattat cggcaccctg ccggtggtca ttcctgtctt
    42421 tgccaatctg ctttatagcc agcgcgacgg caaactcgcg tggggaaaac tcgccccggc
    42481 actgatttgt attggcatcg gcctggcgag tgtgaatatt gctgagttaa accacggact
    42541 ccccgatttt gactgggcac gttatacctc tggcatcgtg ctagcgttag tttccgtggt
    42601 ctgctgggca tggtatgccc tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca
    42661 tccgatgatg tgggcgacgg cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta
    42721 tctcgtcgcc tgttactggc tgaatataca aacgccggac ttctccttac cttttggccc
    42781 ccgtccgctg gtgtttatta gtctgatggt tgcgatagcc gtgctttgct catgggttgg
    42841 cgcactctgc tggaacgtcg ccagccagcg attaccgaca gtgattctcg ggccgctgat
    42901 tgttttcgaa acgctggcag gtttgctgta caccttttta ctccgccagc aaatgccgcc
    42961 gctaatgacg ctgagcggta tcgcgctgtt agtgattggc gtggtcattg cggtcagagc
    43021 aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt tgacacgctg gcagtgagtt
    43081 aaataagcct ctgctacgta agggttatag cttttgcctt aaagatgcat ttaaaataca
    43141 tcttatctta ttaagaatga ggtatcagct atggcttatc gcgaccaacc tttaggtgaa
    43201 ctggcgctct ctatccctcg cgcttcggct ctgtttcgta aatatgatat ggattactgc
    43261 tgtggcggta agcagacgct ggcgcgcgcg gcggcacgta aagaactgga tgttgaggtc
    43321 attgaagctg aactggcaaa gcttgctgaa caaccgattg agaaagactg gcgtagcgcc
    43381 ccgctggcag aaatcattga ccatatcatc gtgcgctacc acgatcgtca ccgcgagcag
    43441 ctgccggagc tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga caaaccgagc
    43501 gtgccaaaag ggctgacaaa atacctgact atgctgcatg aagagctttc cagccacatg
    43561 atgaaagaag agcagatcct cttcccgatg atcaaacaag gcatgggcag ccaggcaatg
    43621 gggccaatca gcgtaatgga aagcgagcac gatgaagcgg gcgaactgct ggaagtgatc
    43681 aaacacacta ccaataacgt cacaccgccg ccagaagcct gcaccacctg gaaagcgatg
    43741 tataacggca ttaatgaact gattgatgac ctgatggatc acatcagcct ggaaaacaat
    43801 gtactgttcc cacgcgcgct ggcgggtgag taatctaaaa gctggatggc attgcgccat
    43861 ccagcatgat aatgcggtta tttccgcagt tcagcagccc gcttcttacc aataaacaac
    43921 cagcccagcc ccagtgcgat aaaccacagc ggggtgacca acagcgcctg gcgagtgtca
    43981 tcttccagtg tcagcaacac gaccacgaac acaaagaacg ccatacatac ccagcacatc
    44041 agcttgccga gcggcatctt atagatcgac ttctcatgca gatgaggacg ctgtttgcgg
    44101 tacaccaggt acgagcaaag gataatcgtc cagacgaaca taaacagaat cgcggaaacg
    44161 gtcgtgatca tcgtgaacgc gccaatcacg ctgggattca catacagcat caccacgccg
    44221 ccgagcagac agatacacga gaaggtcagc cctttcgcgg gtactgcgcg cttagagagt
    44281 ttagcgaacg ctttcggtgc aacaccttcc tgggccaggc caaacagcat acggctggta
    44341 gagaagacgc cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa gttgatcacg
    44401 ctggcagccg caggcaaacc caccaacacg aacagttcaa caaacgggct tttctccggg
    44461 actaccgaac tccacggcgt cacggacata attacaatca gcgcgaagac gtagaacata
    44521 atgatacgga tcggaataga gttaattgca cgcggcagtg atttctccgg atctttggtt
    44581 tccgcagcgg ttgtacctac cagctcaatc cctacgaaag cgaaaaccgc tatctggaat
    44641 ccggcaaaga agccacttaa acctttcggg aaccagccgc catcattcca caaatgcgcg
    44701 aatgacgctt ccacaccagt cggtgactga aagtgcatcg ccaccataac caggccgacg
    44761 acaatcagcg acacaatggc gacgattttg atcatcgcaa accagaattc catctcaccg
    44821 aacattttca cggtggcgag attgagcgtc agcagcagca ctatcaccgc cagcgaggcg
    44881 acccagtcgg agagatcggg gaaccagaac tgagcataag ccgtgatcgc caccacgtct
    44941 gccataccgg ttacaaccca gcagaaccag taagtccagc cggtgaaata tcctgcccac
    45001 ggcccgagta aatcggaagc gaagtcacta aaagatttgt attccagatt cgaaagcagc
    45061 aattccccca ttgcccgcat cacgaaaaag agcataaaac cgatgatcat ataaacgaaa
    45121 atgatcgacg gcccggcaag gctaatcgtt ttgccagacc ccataaacaa ccccgtacca
    45181 atggcaccgc caatggcaat aagctgaata tgtcggtttg tgagattgcg ccgtagcgac
    45241 tgttcagccg gagcctgatc atcggcaacg acttttacct gatctaccat gtttttttct
    45301 tcctgtacct gtctgtgttg ttcaggctct acggccttta gtcagtctat gatacgacga
    45361 atctgtatcg ggactcatcg atattaggta acaatacgcg ggatgaatac taagatttag
    45421 atctaatgtt aattttatgt ttaaagtgag tgtcatatca cggtgataac gcgaaacagc
    45481 tcacaaaaat acacgcttac ggggtctttg caacacaaaa tagcgccgtt tggcaaactg
    45541 aagggtttat tgctgaatgc ctgctcccct ctggttcgag gggaacagaa tgcgctgctt
    45601 agaggatttc cagcagttcg acttcaaaca ccagggtgct gaacggaggg atggatgcgc
    45661 ctgcgccgcg ctcgccatat gccagttcct gcgggatagt cagttcccat ttagaaccta
    45721 ccggcatcag agtcagtgct tcaatccagc cagggatcac gccattaacc ggaaattcag
    45781 cgggttcacc acgagcaacg gagctgtcaa acacggtgcc gtcgatcagt ttaccggtgt
    45841 aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc ttcaccctgg ttgatcacgc
    45901 ggaattgcag gccagattcg gtgctattca caccttcttt tttggcgttt tcttccaggt
    45961 atttcacacc ttcagcagcc atcgcctgga aacgctgacg acgaacggca tcggcgcgct
    46021 cgtggatttc acgcagcgcg cgatgcacca catcaaccgg aacagccgga tgtttgcctt
    46081 ccagcgcatc ggcaatacct gcaaccagtg cttctggcag cagcccttcc aggccagatt
    46141 cactcagttg ttgccctacc tgcaaaccaa tgccgtagct tgcttgcgct tcgatggtgt
    46201 caaaagttgg ggtggtcatc gtatttcctt tcttagggta taaaagtagc gggcagcata
    46261 tcagccacac ttctttgggt aaaactttgt cgcaggaatg atgacaaatc ccggggaaaa
    46321 agaaacaata gcaatatccc gtatgaatat aataaaggcg ggtttaccac ggacatcata
    46381 gcgttagctt attcataggc tatgattgag gaacaagacg cggagcagga ggaaaccatg
    46441 cccgggcgct ttgaactaaa accaaccctg gagaaagtct ggcacgcgcc ggacaatttt
    46501 cgcttcatgg acccgctgcc gccgatgcat cgtcggggga tcattattgc cgccattgta
    46561 ttggtggtcg gatttctgct tccatctgat gatatgccca acgcgccggt ggtgacgcgt
    46621 gaagcgcagt tagatattca gtcgcaatca caaccgccga cagaagaaca gctgcgggcg
    46681 caattagtca ccccacaaaa tgatcctgat caggtggctc cggtcgctcc agaacctata
    46741 caggaaggcc agccagaaga gcagccgcaa actacccaaa cgcaaccatt ccagccagat
    46801 agcggaattg ataatcaatg gcgctcttac cgtgtcgaac ccggtaaaac gatggcgcag
    46861 ctgttccgcg accacggact gcccgctacc gatgtctatg caatggcgca ggtagaaggc
    46921 gcgggtaagc cgctgagtaa tttgcaaaac gggcaaatgg tgaaaatccg ccagaacgcc
    46981 agcggcgtgg tgacgggctt aaccattgat acggggaata atcaacaggt cctgtttacc
    47041 cgccagccgg atggcagttt tattcgtgcg cggtagatga agggtcaacc aaaagccagc
    47101 taaggattgt ctctttgatg accttacgtc tggcttccag tgcctggggg gaaaccatac
    47161 tgatatgata aaccaggcca agcgtatgct ggttgatcaa ataatatccg ccaagcgcag
    47221 caatattaat attcacctgc aatgggtcga tatctggctt aaaaatattg tgtttcttcc
    47281 cttcatccaa taaggattcc attaactgca agtgcgcatg gttaatttct aagagacgct
    47341 gtgattttgc ataatgcaca cctttacttt gattttcact atgcacaatt tttaaaaacc
    47401 aggggttagc aatataataa tcccatgtaa aatcaataat tttctcaatg gctttttgtg
    47461 gcggaagatc tgagaaatca agggcctttt ctttttgtcg aatatcgttc caggtatatt
    47521 ccagtacttc aataaataaa ttttctttat taccaaagtg atgataaacc gtctgtttac
    47581 tacaccccgc agccttaaca atattatcga ccctggcccc ttcataacca tattcggcaa
    47641 attcattaac cgcgcataac agcagctttt ccttcaggct ctgtacgttg ctggtatcaa
    47701 gatacataac gcttccccac gacctcttca agataactat cttaacccga ttaattagaa
    47761 gctccagtaa aaaggataaa aacttgtact ttcatcaagt tatcaaatgt actgtgatca
    47821 ctatcgcata tcaaccaacc agatggttga tctttcttgc ggcacgtcgc aagctaattg
    47881 tcacttagaa aacaatattc aaaaaaagag agctattgta tgaaatccac tcgtcctgtt
    47941 gccgtgatta ccggagccgc tcgtggtatt ggaaagggct gtgcgctgga gcttgctcgt
    48001 ggcggattta acctgctgat taacgatctt cccgatgccg acagcgtaga aaaactgcat
    48061 atcacacaac aagaatgtat agctgaaggc gtggaagtga tttgcttccc tgccgatgtc
    48121 ggcgatctct ccttgcatga agagatgctc gacgcggcgc aaaaccagtg gggacgtctg
    48181 gactgcctgc tcaacaacgc aggcatttca gtgaaaaaac gcggtgacct tctcgacctc
    48241 gaaccagaca gcttcgacca aaatattgcc attaacaccc gcgcgccgtt cttcctcgct
    48301 caggcattca gtaaacgtct gctcgcacag ccgaaaccgg aagcagaact gccgcaccgc
    48361 tcgattattt tcgtcagttc catcaacgcc atcatgctgg cgatgaatcg cggcgaatat
    48421 accattgcca aaaccgccgt ttctgccgct gctcgtctgt ttgctgctcg cctttgtaat
    48481 gaacaaattg gcgtttatga agtgcgcccc ggtctcatca aaaccgatat gactattccg
    48541 gcaacggctt attacgacga actgattgcc aaaggtctgg tgccgtgggg acgttggggt
    48601 tatccggctg atattgcttc cactgtccgc gcgatggcgg aaggtaaact gatttacacc
    48661 tgtggtcagg cggtcgccat cgacggcggc ttaagtatgc cgcgcttctg aggctgaatc
    48721 atgagagatc tgttacaacg cccggattta ttcagtatca ataccgcaac actgggctat
    48781 aaaacgccgc tacccgccat tattgatgcc tgcgccgcgc gtggtattgg cgcgattgct
    48841 ccctggcgca gggagttgca aggtgaagat ctccagcaaa tcgcccacca gcttgccgcc
    48901 agtaacatga gcgtttccgg tttgtgccgt agcacttact acaccgcgcc gacactggcg
    48961 gaacgcaaac tggcgattga tgataaccgc cgggcgctgg acgacgcagc ggtgcttaac
    49021 gccgcctgtt atatgcaggt ggttggcggt ctgccaacgg gcacaaaaga tctgtacgaa
    49081 gcgcgggaac aagtaaaaca agggattcgc cagttgctgc cacattcgaa agatgtcggc
    49141 gtgccaattg cgctggagcc actgcacccg atgacagctg ccgatcgctc ttgcctgtgc
    49201 acgttacgcc aggcgctgga ctggtgtgat gaactcgatc ccgatggtga gtttggcctc
    49261 ggcgtggcgg tggatgttta tcacgtctgg tgggatccgg atctcgccag tcagatcctg
    49321 cgtgccggaa aacgcatcct cgcgtttcat gtttccgact ggttagtgcc gaccactgat
    49381 ctggtcaatg accgtggtat gccgggagat ggcgtcatca atattccgtc aattcgtcgg
    49441 ctggttgaaa acgccggatt taatggtgct attgaactgg aaatattctc gccttactgg
    49501 tggcagaaag atattaacag cacgctggat attagcgtcg atcgcatcgc gcattattgt
    49561 taaggaatga tcatgagtga ccaacccgtt ctgttcagca gagtggcggc ttcctgccgc
    49621 ctgacattga accgtgaaga taaatgtcat gctattaacg aagagatgat cgaatctctc
    49681 gaccattatt taaatgaaat tgaaaacgac accacattac gattagtcga attaacggca
    49741 accggcgata aattcttttg tgcgggcggc gatattaaat cgtggtcggc ctattcgcca
    49801 ttagatatgg gcagaaaatg gattaaacgc ggtaatgacg tttttaatcg cctgcgaaac
    49861 ataccccaat taaccgttgc gaatctcaac ggtcatacca tcggcggtgg tattgaactg
    49921 gccttgtgct gcgatattcg tattgcccgc cccggcgcga aattctctaa cccggaagtg
    49981 atgctcggca tggtgcctgg ctggatgggc attgaacgtg tgcttaatca ggttggtccg
    50041 gtggtcggtc gccagatgct gatgctgggt aaacgcctga cggcgcagga agcccaggcg
    50101 gcgaatttaa ttgatgaagt ggtagaaaaa gaacaggtgg aaagctggat ggcgaaccag
    50161 ttagcgcagc tggaaaaatg cggtccggtg gcactggcgc atattaagca actgattctg
    50221 gcactggaaa ataaacatgc cgattacccg caccagctac tggccggatt aatgtctgcc
    50281 actcaggatt gccagcaggc gacgcgcgcc tttgccgaaa aaagtagcgt gagcttccat
    50341 aatcaataag gaaattgtat gcgtaagata acaaccgccg aggcactggc ggcgcaaatt
    50401 caggacggtg cgactattgc tattagcggt aacggcggcg gtatggtgga agccgaccat
    50461 attctggctg ctattgaagc gcgtttcctg caaaccggac accctcgcta cctgacgcta
    50521 atccactcgc tgggtattgg cgatcgcgac tgcaaaggca ctaaccgttt tgcgcacgca
    50581 gagatgctca aacgcattat tgccgggcac tttacctggt cgcccaaaat gcaggcgctg
    50641 gtaaaaaata acacgattga agcctactgc tttcctggtg gcgtgattca ggcactgcta
    50701 cgggagatcg gcgccggacg tccggggctt tttacccacg ttgggctggg atcgtttgtt
    50761 gatccacgca atggcggcgg taagtcgaat gaatgcacta ccgacgagct ggtagaactg
    50821 attgaaatcg acggtgaaac caaacttcgt tatcgccctt tcaaagtgga ttacgcgatt
    50881 ttgcgtggca cttatgccga tcctcgaggc aacgtcagcc ttgaagaaga agcgattgat
    50941 atggatagct attccatggc gctggcagca cacaacagcg gcggcaaagt gttcgtacag
    51001 gtacgcgatg tgctggaagc tggcgcgatt gaaccacgcc gggtcaaatt accgggcatt
    51061 ctggttgatg gcatcgttga acaccgcgaa caaccacaaa cctatcttgg cggttacgac
    51121 ctgaccatca gcggccaaca tcgccgtctg agttctaacg acgccattga actggttagt
    51181 catccggtac gtcgcctgat tgcccgtcgg gcggcacgcg aactggtagc tggcgcttca
    51241 accaactttg gctttggtat tcctgggggt attccaggcg tagcgctgcg cgaaggcgtg
    51301 ccttaccaaa gtttatggct gagcgtcgag caaggcgtgc ataacggtat gatgctggat
    51361 gatgcgtttt tcggctgcgc ccgcaacgcc gatgccatta ttccatcact cgatcaattt
    51421 gagttctaca gtggcggagg gatcgatatc accttcctcg gcatgggaga gatggatcag
    51481 tacggtaacg tcaacgtatc ccatctcaat ggcaatctca ttggtcccgg cggttttctc
    51541 gaaattgcgc aaaacgcccg taaagtggtg ttctgcggca cgttcgacgc caaaggtagc
    51601 aagattgatg taacgccaga tggcttgcat atcacccagt caggtcaaat ccctaaactg
    51661 gttacccagg tggaaaaaat cacttttagc gccgcctacg cacagcaaag tggtcaggaa
    51721 gtgttgtata tcactgaacg cgcagtattc cagttaacgg cagaaggcgt tgaattaatt
    51781 gaaatcgcgc cgggtgtgga gattgagcgc gacattctgc cgtatatggc tttccgtcca
    51841 attatcaagc acccacgcct gatggaaagt agcctgttta cgccgatgga ggatgcatga
    51901 gtcagcttga tgacaccatc cttgatgcgc tgacgcacgt tactttccca aagggttttg
    51961 cacaggcaga gcccgcatgg gttgtcacgg tagacggtgt tgattaccca ctctggcaaa
    52021 cagatgccct ggtagtcggc agtggtgcag caggattacg tgcggcagtt gaacttaaac
    52081 gccgccagca aaatgtgctg atcgctaccg ccgggttata tatggggacg tcggcatgtt
    52141 ctggttccga taaacagaca ctatttaccg ccgctaccgc gggcaacggc gacaacttca
    52201 ccaaactggc agaggcactg gcgagcggtg gggcgatgga tcacgacacc gcttatgtcg
    52261 aagcggtagg ttctctgcac actctaggcg ggttgcaata tcttggtctg gaattaccgg
    52321 aagatcgcta tggcgcgatt cttcgttatc aaaccgacca tgacgaagcc gggcgtgcaa
    52381 cctcgtgtgg gccgcgtacc tcaaggttaa tggtgaaagt gctgttggaa gaagtacagc
    52441 gcctcgccat tccagtgttg accagtgcaa cagtgattaa actgctgcat cagcgtgacg
    52501 aaaacggcga agaccgcgtg gcaggggcaa tcctcgcgac cggtcatcgc gcccataacc
    52561 catgggggct ggcaattgtg actgcgccca atgtggtact ggcaacagga gggcctggcg
    52621 agctttatcg cgacagtgtt tatccacata aatgcttcgg ctcgctgggg ctggcgctgg
    52681 aggaaggtct gacgctaacc aatctgaccg aaagccagtt tggtataggc acgccgcgta
    52741 gcacgtttcc gtggaattta tccggcacct atgtacaggt gatcccgtat atctattccg
    52801 tggatgctga gggtaacgag tataacttcc tcgcggatta ctatcgcacc acccaggagc
    52861 tggcttcaaa cattttccgt aaaggctatc agtggccgtt ccacgccacg cgggtgatgg
    52921 attttggctc cagcttgtta gatatggcag tagcgcaaga gcagcaatca gggcgtcagg
    52981 tatttatgga tttcaatcgc aatcctgaag cggtgccggg tgacctgccg ttctcattag
    53041 atcgactgga cgacgacgtt cgcgcgtatc tggaaaataa cgacgctctg gcaccatcgc
    53101 ccatcgaacg gctgcaacga atgaatccgc tgtctatctc gctgtataag atgcacggtt
    53161 acgatctcac cacacagcct ttgcagtttg ccatgaataa tcagcatatg aatggcggca
    53221 ttgaagtgga tatctgggga caaacctccc tgcccggctg tttcgccgtg ggggaagttg
    53281 ctggcacaca cggcgtcact cgccctggtg gtgcagcatt gaatgccggg caggtttttg
    53341 ctgttcgtct ggcacgtttt attggttgca cgcaaaagcg gaatattaat ggagatatag
    53401 cacagctggt agctcctaca ctggcttcta taagagagat cattactcag gcgcacgata
    53461 acgggaccgg gatgccgttg tcggttgtga gagaaaaaat tcaggcacga atgtctgacc
    53521 atgcgggatt tatttgccat gccgataaag tccgacgcgc cactcgcgat gccctgctat
    53581 tgagcgaatt tgtccaacgg catggattgg ctatcaaaca tgtgggtgaa gttgccgagc
    53641 tgtttatgtg gcggcatatg gcactgactt ctgccgccgt attaacgcaa ttaacgcatt
    53701 atattgacgc aggtggtggt agtcggggag cacgtattat tctcgacagg gatggaaatt
    53761 ctattccgca aacacgaaat ggtttttgtg atgcctggcg ctttcgttca gagcgtactg
    53821 aggataaaaa agataaacta cttattcatt attgcaacgg tatctttcac gtcagagaaa
    53881 cacctgttcg tgagtttcct attatccgag gtatttggtt tgaaaaaaac tggcctggtt
    53941 ttcttaatgg gacaatttac cagccacagg atgaatgata ttgcaaaggt taatcactat
    54001 gcgaaatata attagatcta ttaatgctct acttgcagca ttgaacatta ccattttggc
    54061 gatcatcgtg gcatgtgtga cgtggcaggt ggctgcacga tttatattta ctagtcccag
    54121 tatttttaca gatgaactca gccgtttgct ctttatttgt cttggacttt ttggtggggc
    54181 atacaccgca gggcaaaacc ggcatctggc gattgatctg ctacctatga tgctgaaagg
    54241 aaaagcccga cgtcatttat ttttgtgtat tcagattatc gttattatct tcgcaaccat
    54301 cattatggta tatggtggtg ggctattaac gatggataca ttcgattcag gtcagacctc
    54361 gcccgcttta ggctggcaaa tgggatatat ctatatgagc atcccgatta gtggtgtact
    54421 tattattata tacaccatag atatggtttt aaccgaactt aaacaaccac tctaaagata
    54481 acaaacaaca accaaccata gtaggaatgg agaaattatg gattactggt taccgattat
    54541 tgtgttattt ggtgccttct tttttatgtt ggcattaggt gtacctattg tttatgccat
    54601 tggtttatcg acactggcat caatttccac acaactcgat ttcaactcag cattgagtgt
    54661 ggtttcccaa aagttagcct ccgggttaga tagttttacg ttgttggcaa tcccattttt
    54721 tatcttgtct ggcaacatta tgaaccatgg aggaatagcg cgacgattaa tcaactttgc
    54781 ccgtattctc ggcggtcgtc tgccaggctc acttgcccac tgtaatattc tcgccaatat
    54841 gctgttcggg gcaatttctg gttcagccgt ggcttcggcg gctgcgatgg gcggcgtcat
    54901 gcatccgcaa caggttaaag agggttacga cccggcattc agtactgcgg ttaacgttgc
    54961 ctctgccccg acgggtttgc ttattccacc aagtaatacg ttaatcgtct attcactggt
    55021 cagtggtggt acttctattg ccgcactgtt cctggcgggt tatgttccgg gaattttgct
    55081 gggtctggca ctgatggtaa tagccggtat tatcgcggta cgtcgcggtt atccaaagcc
    55141 agaacgcccg actttacgcc aggctggcgt tgcgatatgg atggctatcc ccagtatttt
    55201 cctgattatt ttaattatgg ggggtgtatt aagtggtatt tttaccccga cagaggcctc
    55261 cgcaattgca gtaatttata cccttttcct tgcactggtt ctttatcgtg aaatctcagt
    55321 taaagattta cctaaaatat ttctggaatc cgttattacc actgcaattg tcttgttatt
    55381 aattggttct tcgatgggta tgtcatgggc aatgtcaaac gctgacgttc ccttcctgat
    55441 tctcgatttg cttaacacta tttctgacaa cccaataata atcctgctta ttatcaatat
    55501 tatcctgttg attatcggca cttttatgga tatgacaccg gcggtactga tttttacgcc
    55561 gatattttta ccggttgtca ccgaactggg catggacccg attcactttg gtattgtcat
    55621 ggtactgaac atgtgtatcg gtatttgtac tccgcctgta ggcagtgtgt tatttgtcgg
    55681 ttgtagtgta tctaaattac cgattaataa aattatcaaa cctatgctgc cattctatgc
    55741 cgtgatggta ttggtgctgg caatggtgac ctatattccg caaatcagta tggcattgcc
    55801 aagagcactg ggctactgat gcgcattcac tggcggcacc tgccgccata tttattagaa
    55861 aaggaacatc tatgaaaata aaagtatccg ctggcattat cggtgctgtt cttatgttat
    55921 ccgcaagcca gtcctgggca gtgacattaa aactgagtca taatcaggat aagtctcatc
    55981 ctgttcataa agcgatggag ttctttgcga aaaagagcaa agagtactct aacggtgata
    56041 ttactattcg tatttatcca aatggaacat tgggtactca acgagaaaca atggagctga
    56101 ttcgttctgg cgctattccc ctggtaaaaa ccaatgcggc agaaatggaa gcatttgaaa
    56161 attcctataa attatttagc ctgccttatt tgttccgcga tcgtgatcat tattatcagg
    56221 tcatgcaggg cgatatcggg agaaaaatcc tcgactcaac gaaaagcaaa ggttatttcg
    56281 ggctgacttt ttatgatgga ggcgcccgca gtttctatgg caataaacca gtactgaaac
    56341 cagacgatct gaaaggcatg aaagtccgtg tccagccaag ccctggcgca gttgaaatga
    56401 tcaaagtcat gggcggtaac ccgacgccac tggattacgg cgagttgtat acagccttac
    56461 agcagggtgt ggtcgatatg gcagaaaaca gcgtgatggc gctgactacc atgcgtcacg
    56521 gtgaagtggc aaaatccttc agccttgacg aacacactat ggttcccgat gtggttctga
    56581 tgagcaatgc tgcgtttgat aaacttagcc cggaaaatca ggcagttata ttaaaagcag
    56641 ctaaagaatc aatgagctac atgaaagact tgtggagcga ggaagagaaa caagaatttg
    56701 caaaactgga taaaatgggc gtgaaagtct accaggtaga taaagctccg tttatcgaga
    56761 aagtacagcc aatgtacgca aacttcgcta aggacaaccc agcccttgcc ccaatgctgg
    56821 ctgatattca ggcagctaag taattatctt cgtagggctg cttattgtgg cccttatcga
    56881 tatctgcaag tgctcgctac aaaagcaaaa cgccgaccaa tggtcggcgt ttttacgtct
    56941 cgttgaataa cgaattattc agctactacg tttacgatca ctttcgcgaa tacttcgctg
    57001 tgaacctgga agctcacttc gtgttcgcca gtggtacgca gaacgccgtt cggcagacga
    57061 acttcgctct tagccacttc aacgccagct gcagttacag cgtcagcgat gtcgcgagta
    57121 ccgatggaac cgaacagttt accttcgtcg ccagctttag acgcgatggt aacagtttcc
    57181 agtgcattga ttttctcagc gcgagcatta gctgctgcca gaacttcagc cagtttagct
    57241 tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt tcttggtagc tggaacagct
    57301 ttaccctgcg gtaccaggaa gttacgagca tagcccgctt taacgtttac ctgatcaccc
    57361 aggctaccca ggtttgctac tttatcaagc agaataactt gcattacctt atcctctcaa
    57421 agtcgtatta atggaccgtg accgattact gatggcgatc agtgtacggc agcagggaca
    57481 ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg gtattttgca cgggtaccgg
    57541 tgatacggct tgggacaatc ttaccgcttt cggtgatgta gtttttcagc gtagcgatat
    57601 ctttatagtc gatctcttga acgccttccg cggtgaaacg gcagaacttg cgacgacgga
    57661 aataacgtgc catatggcta gtctccagaa tctatcaatt caatctgctc ggcatgcaaa
    57721 accattttgc tcagtccgtt ctttgccttg tggcatgaaa tgaacccctg aacggttatg
    57781 cgactgccga ccgttatact gtgagtaatg gcctggtttt cgtgtccgct aacaataacg
    57841 ggcatttggc accacgcctg ccggtgaaag ccggcttcct cctgcacaga acgatgctca
    57901 agcacgaact ggcagtgagg aattcctgat ggactgacct ttcgaagggg agtcctgcac
    57961 acggtgccgg acaacaccag acggttggtc atcagaaatt actcttcaga atccccagct
    58021 tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac gctcacggcg ctcgtctttc
    58081 gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg tacgcataac catgctgcgg
    58141 ataacggcat cgttgaagcg gaaggtagtt tccagctcat cgatcacttc ctgcggagct
    58201 tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt tgatcgggta agccagctga
    58261 cggcggcccc agtcttccag acggtggatc ttgccttctg caccagtgat ggcagcagtg
    58321 tagcgctcga tcatgcccgg aacctgttcg ctctggtcag gatggaccat aaaaacgatt
    58381 tcgtaatgac gcatcgaatt gctccttacg gattattcag cctcctgtct gggtcagccg
    58441 aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa aaatgacgcg ttatagtact
    58501 tgtatgcccc gctaaactca agggcagagc cgaaataaat ctcccctttc gcaattaacc
    58561 tctaaaccgc tgatttggag ttcatcacac ttcatcatgg tgtttttttg aacaactgca
    58621 tcagaaatgg tcaccatcaa cttctttatc attgattaaa attaaatcag aagatcatca
    58681 cagaggagag cacacaaagt cagccgtcga ggaggtatct atgttcagtc gagttttagc
    58741 ccttctggct gtgcttttgc taagtgcaaa tacatgggca gccattgaaa tcaataacca
    58801 ccaggccaga aatatggacg atgtgcaaag cttaggcgtg atttatatca atcataattt
    58861 cgccactgaa agtgaagcac gtcaggcatt aaatgaagaa acagatgcgc agggcgcaac
    58921 gtactaccac gtaattctga tgcgggaacc ggggagtaac ggcaatatgc acgccagcgc
    58981 ggatatttat cgctagcacc aggtataacc aacgaaacat tgccatagtt tgctttgccc
    59041 ccttcgcagg gggctttttt tgaaaaacgg ctgcggaatt agaccagtta tctcccgaag
    59101 aaggaaattt ccgcagcgcg tgttttactt ctgcccgtaa taagcgttag gaccatgctt
    59161 acgcatgaag tgtttattca tcaggaagct gtcgatgtga ttgagttgtg gattaatact
    59221 gcgcgcaatc cacgccattt tcgccacttc ttccatcact accgcgttat gcaccgcgtc
    59281 gtgggcatct ttcccccagg cgaacggtcc gtgctgatac accacaattc ccggcgtatg
    59341 cagcggctcg gcgttgccca gcgtttcgat aatcactttg ccagtgttca gttcatactc
    59401 accctgcacc tcttcttcgc ttaatccacg cgtacacggg atgtcaccaa agaagtagtc
    59461 agcgtgcgtg gtgcctaacg ccgggatcgc cagccccgcc tgcgcccatg cggtagcatg
    59521 agtggagtgg gtatggacaa tgccgccaag cgacggataa cgacggtaga gttcaagatg
    59581 cgtcgcggtg tcggaagatg ggcgatactc cccttccacc accttaccgc tcatatcgac
    59641 caccaccata tcgtctgctt tcatggtttc gtaggcgacg ccgctgggct tgatcaccac
    59701 cagcccgcgt tcgcggtcga tagcgctgac gttgccccag gtaaaggtca ccagcccgta
    59761 gcgcggcaga tccatgttgg cttcaaatac ctgctgtttt agcttttgca ttatgccgcc
    59821 tccaccatgc ccgctttcgc catgcgcgct ttcacccaat cacgcgcttt cgccacttct
    59881 gccgccgggt cttccgccgt ttcgctccac atctcaatca ggtacggccc gcaatagcca
    59941 ctctgtttga gcgtttcgaa acaacgttcg aaatccacta caccttcgcc aaacggcacg
    60001 tttttgaaga cgccaggttt ggtgtctttc acatgtaccg cgacgatatg cccgattccg
    60061 gcctgcaact ccatctgcac atcgttgtcc cacgccgaca ggttgccgat atccgggtag
    60121 agctggaacc acgggttgtt gagatagtgc gcgtaaccca gcgccttgct gatggagttc
    60181 atcaacggat aatccatgat ctccatcgcc agcgtcacct gcgcgcggct cgccatctca
    60241 acgctctctt tcaggccgtc acggaaacga cgacgcgttt cgttattggc ttcctgatag
    60301 taaacgtcat agcccgccag ctggatcacg cgaatgccga cgtcctgggc gaactggata
    60361 gctttacgca taatctccag cccctgcgcc cgcaccgcgt catcttcact gcccagcggg
    60421 aaacgacgat gagcagaaag gcacatggac ggcacgcgca cgccggtttc aacaatcgca
    60481 ttcaccagcg ccagacgctg ctcgcggctc cagtcgaggc gcgacaggcg ctcgtccgtt
    60541 tcatctaccg acatctcgac aaaatcgaag cccaacgttt ttgccagttg caggcgttcc
    60601 agccagcact ccccggcggg gagcgctttt tcatagatgc caagcgggat ttgtttggac
    60661 aacatatccg ctccttagcc ccacagttcg gcgatggaac gtttgaactg acgtgcagct
    60721 tcaaccggag aagcggcatc acggatgctg cgaccagcga taaacacatg aatcggaata
    60781 cctttgaaca gcggcagatc ttccagcgcc aggccaccgg tgacggtgac tttgaagccc
    60841 atatcggaaa gacgtttgat cgcggtaatg tccgcttcgc cccacgctac gcctgcggcc
    60901 tgcgcgtcac ggctgcggtg ataaaccacc tgctgaatgc ctgcgtcacg ccactgctgc
    60961 gcctgttccc aggtccagta gccggtcaat tcgatctgca cgtcgccgtt aaactctttt
    61021 gctacgtcca gcgcgccttt ggcggtgttg atatccgcac agcaaattac cgtcacccag
    61081 tcagcgttgg cttcgaagca catacgcgaa aggattttgc ccgcatcagc aattttagcg
    61141 tctgccagta cgattttgtg cgggtagagc gctttcaggt cacgaaccgc acgcacgcct
    61201 tcgcccacgc acagaatggt acccacttcg ataatgtcga cttcttcggc aatcaggcga
    61261 gtggtttcgt aggcgctatc catagtctgg ttgtccagcg cgacttgcaa catcggtaat
    61321 gacatcttca attccttctt aagctgccgc gttggtgcgg tcaatgagat ccagtacttc
    61381 ctgctcggta cggcaggcac gtaaacggtc gaaattttct tcatcttcaa acaggttgac
    61441 gatctgcatg atgcccactt cctggtgcgt gttggcatcg accgccgcca tggtgatgag
    61501 gatatccacc gggtcgttat cttcgtggtt gaactccagc ggctttttca gcgtcaccag
    61561 cgcaaaaccg gttttcttaa cgccctcttc cgggcgaccg tgcggcattg ccaggcctgg
    61621 tgcgagcaca aagtacggac cgtgctgttc aaccgcatcc agaatcgcct ggtagtaacg
    61681 cggctcgaca acatccgccg ccaccagcag gtcaacgccg atttttactg cgtcctgcca
    61741 tgtctcagct tcagcctgca ggcggatgga tttattctcc gccagcgaat cacgtaattt
    61801 catggcgcgt ccttacttca catcctgcgg gaaatgcgct ttgatcactt ccagcagttt
    61861 cgggccaaag tcggcaggag agagcatgtt gcgcacgcca accacgtatt tgttgccggt
    61921 cacggtgatt tcgcccgcaa tgtgcgtaga agcgatgatg atatccgcgc cgctcaactc
    61981 gcttttgtat tcgccaaccg cgcagctatt taccgtgtgg tcaatgtttg attgggttaa
    62041 aaactggtcc actttcatct tcatgatcat ggaactgcct tgtccgttgc cacacacagc
    62101 cagaatacgt acggtcataa tcaaaactcc ttattaagca gactgttctg ccagttgttt
    62161 ttctgcatct tcttctgcgc gcagtgcgcg gccagcgaag aacatataag ccagtgcaat
    62221 tacaatgatg acggccataa aggcgatacc gatggagaag aagccctgca tcatcggcgg
    62281 tgccagaatc gaccagtccg ccatgcccat ccaggcgctc ataccggtga gtttcaccgc
    62341 ccagacgcag ccaaagattt caatcatccc catcaccaga catatcttca gcgccgcacg
    62401 ccagccgccg aagtggttag cgaacacgcc gatggtggcg ttggagaaga acatcgggat
    62461 aaagccggga ataatcagga tcgaggaacc gcaggcgacc aggatgccaa ccgcaatcag
    62521 ctgaccgatg gtgccccaca taaagcccca aaccacggcg ttcggcgcga agctatagat
    62581 agccgcacag tcaatcgcca gaaccgcacc agggatcagg cgctgggaga taccgttaaa
    62641 tgcttcagag agttccgcca caaacatgcg cacgccctga gtgatgatga agatcgccac
    62701 cgcgaaggag aaaccagttt gcaggatgta caccgtccag ttcactttgc ctgccatcgc
    62761 ctgcacggtg tcgataccga aggagagcag aatggcacca aagaagatgg tcatcacaat
    62821 cgccgtggag acgatgttgt cgtggaaaat attcagccag cctggcagtt tgaggtcttc
    62881 aacgctttcc tcttttttgc cgaggaacgg cgcaacttta taggcaatcc atgatgcaaa
    62941 ctgttgctgg tgaccgatgg agaaaccgca gccgtccgtc acttcctgag tcggcttgta
    63001 catcatgttg gaggtaatgc cccagtagag cgaaaccaga atcgccgtgc agataatggt
    63061 tgtccacatg gagtagccga aaataaacaa cgtaacggca atcagccccg cctgctggaa
    63121 catgatatga ccagtcagca tgatggtacg aataccggta atgcgacgca gcagcacgta
    63181 acagatgttc agcgccagcg ctaacaacac cgcgtaacct acccagctat aggcatcgcc
    63241 catgcggtcg atggttgcca tcatcgacgc gtaagtatcg gaaattgcgc cgttaatgcc
    63301 gtagacttcg gacattttcg ccaccaccgg tttgaaggtg ctggtgagga tgccggaccc
    63361 tgcctgcaac aacatgaaac caattatggt tttaatcgtg cctttaataa taacgctgac
    63421 acttttgcgc agtaggatgt agcccagaca ggtcacaata cccagcaaca acggggcatt
    63481 ggtcatgacc tggttaaaaa acacggtaaa gatgttgtag aggatctcca taacgatctc
    63541 ctgaagaaga ggtaaccggg tattccacac acccggaatg ttgtgcactc actctaattt
    63601 tcaaaagtaa tcacaacaag attatttatg attaaatgtg acgcgacccg caaataattc
    63661 cacatggata gaatgggatt cacacaacga aacaactatt catttaaatt catttagtta
    63721 attgaattac acattccatt aaatctttcc acactcaaaa tcaacaacca cttccctggc
    63781 aaaaagtcaa aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt aatcaaaaca
    63841 aatcacatat tgattcgaat tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa
    63901 agtatcaccc gagaatcctg gatcctgagc actttcccgg agtggggtag ctggctgaat
    63961 gaagaaattg aacaagagca ggtcgcccct ggcacatttg cgatgtggtg gctaggctgc
    64021 accgggatct ggttgaaatc agaaggtggc accaacgttt gcgttgattt ctggtgcggc
    64081 actggcaaac aaagtcacgg taacccgtta atgaaacagg gtcaccagat gcagcgcatg
    64141 gctggcgtga aaaaactgca gccaaaccta cgcaccaccc cgtttgttct tgatccgttt
    64201 gcgattcgcc agatcgacgc ggtactggcg actcacgatc acaacgatca tatcgacgtt
    64261 aacgtcgctg ctgccgtgat gcagaactgt gctgatgacg taccattcat tggaccgaaa
    64321 acctgcgttg atttgtggat tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa
    64381 ccgggcgatg ttgtaaaagt gaaagacatt gaaattcatg cgcttgacgc tttcgaccgt
    64441 actgcactga tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc ggacggcatg
    64501 gacgatcgcg cggtgaacta tctgttcaaa acgcctggcg gctccctgta tcacagcggc
    64561 gactcccact actctaacta ttatgcgaag cacggtaacg aacatcagat cgatgtggcg
    64621 ttaggatcgt atggtgagaa cccgcgcggt attaccgaca aaatgaccag cgccgatatg
    64681 ctgcgtatgg gtgaagccct gaatgcgaaa gtggttatcc cgttccacca cgacatctgg
    64741 tcaaacttcc aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgt
    64801 ctgaagtatg gcttcaagcc gtttatctgg caggttggcg gcaaatttac ctggccgctg
    64861 gataaagaca acttcgagta ccactatccg cgcggtttcg atgattgctt cactattgaa
    64921 ccggatctgc cgttcaagtc attcctgtaa tctgtcgttt ataccggata gcgtggagaa
    64981 gttcgcgcta tccggcgtag tatttcctgg ctatttcaaa tatcatctaa aaaaatcaaa
    65041 ttttatcgga atagctcatg actgaagcac aaagacatca aatcctcctg gaaatgctcg
    65101 cacaattggg ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca
    65161 ctgcgcgacg cgatatcaat aaacttgacg aaagcggcaa actgaaaaaa gtgcgcaatg
    65221 gcgcagaagc tattacccag cagcgcccgc gctggacgcc gatgaatctg catcaggcgc
    65281 agaatcacga tgaaaaagta cgtattgcta aagcggcctc gcagctggtt aatccgggcg
    65341 aaagcgtagt catcaactgc ggctccaccg cgtttctgct tgggcgggaa atgtgtggca
    65401 agccagtgca aatcatcact aattatctac cgctggcaaa ttacctgatc gatcaagaac
    65461 atgacagcgt gatcattatg ggcggacagt acaacaaaag tcagtccatc actttaagcc
    65521 cgcagggcag cgaaaacagt ctctatgccg ggcactggat gtttaccagc ggcaaaggac
    65581 tgaccgcaga agggttgtat aaaaccgata tgctgacggc aatggcagag cagaagatgc
    65641 tgagcgtggt agggaaactg gtggtactgg ttgatagcag taagattggc gaacgcgcgg
    65701 gaatgctttt tagccgtgcc gatcaaatcg atatgcttat caccggcaaa aatgctaacc
    65761 cggaaatcct gcaacaactg gaagcgcaag gggtcagcat tctgcgagtt taaagatgct
    65821 ggcggaaaaa tgtcacggca gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg
    65881 gctgccatga acaggttagc agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac
    65941 gtagtgattc atcggcaggc acaacgtcat cgtcgaggcc atgccacagc agtagaggtc
    66001 tgtcaccaag ttgttccagg tggtttgtcg cttcccactc tgccagcggc gcgacaatgt
    66061 tattgaattc attctgctgt gctgccgttt caggtatcag cggtggaaac agtgaacggg
    66121 cgagcgatgt aaaatagccc gatcccatca tgctggcggt acatttcacc gtggggtggc
    66181 gagcggtaat tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact gccagacggt
    66241 catcaagcag ccagttttct tcggctattg ccgcacgtaa agtagtgaat tcctgcatac
    66301 tttgtagcaa gatttgccag aattgattta accgccgcgc tgcgtcacca ctaaaacggc
    66361 taccgtgatc gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg
    66421 caaaatagct atacaccaga ctggatgaag taaagccgtg ataaaaaatt acgcacggta
    66481 acggggtatc tttttgcccg acaggataag catgaagaac gggaatatct gccagctcgc
    66541 gtgattctat ttcaatcatc tgtctctcct ttttgtctgg tatgacatga agaaatcctt
    66601 agcacaagtt tctgctggcg gtattagttc ataatgttga gatgtgggtt acgctttcgt
    66661 cacgttttca ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc cccaaagtta
    66721 aattaataag gcactacact atggttagca ggaaacgtaa tagcgttatt taccggtttg
    66781 ccagtttatt attggtgttg atgttaagtg cctgtagcgc actgcaaggt acgccacagc
    66841 cagcaccgcc agtaacggac catccgcaag agattcgccg cgaccagacg caagggttac
    66901 agcgaatagg cagcgtaagc acgatggttc ggggttctcc ggatgacgca ttagcagaaa
    66961 tcagagcgaa agctgtcgct gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa
    67021 ccatcgtgac aggacagtgg tattcacaag ccattttgta ccgtaaataa atcagacaac
    67081 ttacatagtc agctttacat tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt
    67141 accggctgga agcctgggcg tttattaccg cgatggaatg ccctgcaaca tgtggggaaa
    67201 cgaaaaatgg agctgacgat gaaacaatta cttgcctcac cctcgctgca attagtgact
    67261 tatcctgcga gcgccacggc acagtctgca gaattcgcca gtgctgactg cgtgacgggc
    67321 ctgaatgaaa taggtcagat ctcagttagc aatatttcag gggatccgca ggacgtggaa
    67381 cgtatcgtgg cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg
    67441 tacgaagacc agcagcctga taactggcgc gtacaggcga tactgtatgc atgagtgtca
    67501 tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc tacaattttc
    67561 acatatttca tttagttaat cgaaaccagc gtcgcatcag tcgtatacgc ttacacacac
    67621 actccccccg tcgcccgcag caataaatca ttctggatct gctctgacaa ccgtacgccg
    67681 ccgcgcgtat ctaacatcac ctgacaccac gcctgcgcca ttggcggaga agcatatttc
    67741 aacatttgcg caccgcagcc cagcaggaat agctgatgag taatctctcg cctcagttct
    67801 tcagctggct tacgcagctg ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc
    67861 tgccctttca cttccacaaa cgcttccgac aataagtcgt atacgcccgc ttgcttattg
    67921 agaacgcgca gcacatccag gcacataata ttgccggaac cttcccaaat actgtttacc
    67981 ggcatctccc ggtaaagccg cggcagctcg ctctcctcgc aataaccaat gccgcccagc
    68041 acctccatcg cttcggccac aaacggcata cctcttttgc agatcacaaa ttttgccgca
    68101 ggcgtaaata aacgcgccca cagggcttct ttggcatcgg cacgccggtc ccacgctcgc
    68161 gcaagacgaa acagcaacgc cgtttgccct tcaagctgaa gtgccatgcg acttaagaca
    68221 tgacgcataa ggggctgttg gatcaatgga ttaccaaaaa catggcgttg atgtgcatga
    68281 taaatcgcca gcgaaaatgc acggcgcatc atggcatggc tacccagggc gcaatcaaaa
    68341 cgcgtcatcc cacccatttt caggatcaga cgaattcctt ccccttcctg ccccaacaac
    68401 cagccaatgg catcctgaaa ctccacttcg cagctggcgt tagagcggtt acccagctta
    68461 tctttcagcc gctcgaggcg aatcgcgttg cgttgcccgt caggcaaaaa gcgcggcaca
    68521 aaaaagcagg acaaaccgcc cgtagtctgc gccagcacca gatgcgcatc gctttgcggc
    68581 accgagaaaa accatttatg ccccaccagc cgataagagc catcttccag gcgctctgcg
    68641 cgggtggtgt tgctcatgac atcggaaccg ccctgctttt ccgtcattcc catgccaatc
    68701 aacaaaccgc gtttttgccc acctggcaat aagtgagaat cgtagcgatc gctcagcagc
    68761 ggcgtggtcc agtcctgaaa cggcgcgggt aacatctgta acagcaatgg cgtggcggca
    68821 aaggtcatgg ttatcggaca taacgaccct gcctcaacct gtgcgtgcaa cataaaacgc
    68881 gccgcgcgcg ccacaaatgc gccggagcga gcgtcttctt cccaggcaag attgtgcacc
    68941 cgattggtac atagcgcctg catcagcagg tgccaggcgg ggtgaaaacg cacatcgtcc
    69001 agacggcgtc cttgcgcatc gtagcgcaat aattcaggcg gattcacatt cgccagccgc
    69061 cccagttcaa gggattcagc cgttcctaac tgctgaccaa tactggctag aaaatcgcta
    69121 tcccagccag caccttcacg cgttaccgct tcgcagagcg cgccatcaga caggtataag
    69181 ttgctgttat ttaatggtat aggttgatta aaaacggtgt gagtttgcca gtgcactgtg
    69241 tctccctcca tcaatggcag tcaccattag tatggtcact gccatggatt catgacagaa
    69301 agagattgct aaaacattct tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt
    69361 tggtgatcag tgtgttatct tcacggatcc ccattccgca cgcttcatca tcgacaatcc
    69421 agctaccgat gagtgtgtag ctatcgccaa atcgcggcag aggttgaaac gcctggtaga
    69481 tcatcggttc atcggcgtaa tcaccatcag cgtggtcaac gacgttattc tgaccgtcga
    69541 aaatggtgac gtttccgcct tcgcgcgagt agatcggttt acgcacatag ctttcgccag
    69601 cggcaatctg cgatttttca ccctcaaacc aggatgcaag aaggttagga tgaccaggga
    69661 agaagcgcca aagcagcggc attagccctt tattactcaa gatacttttc cataacggct
    69721 ccacccattg ctcgcgacgc ttgcacagca gcggaccgtt atcgtcacgc atcatccact
    69781 ccagcggata cagcttaaat gcacgctgga tgacattatc atcaagatcg gtcagtacac
    69841 cgccgacgcc caaaccgaga tcttcaatgt agataaaccg cgactcctgc cctgcctgct
    69901 gggcgcagtc ctgcaagtac agcacggtag tcctgtcttc atcggtgtcc tgacagcagc
    69961 agaaataaaa cggctcccgg ctataaagct cgctaaagcg tgaaatcagg cgttcctgaa
    70021 tggcattgta ctgatcggca tcacgcggaa taatgccgct gcgccgggca tcctccagcc
    70081 acaaccactg gaaataagcc gactcataca acgaggtcgg cgtatcggcg ttgtactcca
    70141 gcagcttcac cggcgcattg ccacaccagg caaaatccat ccggccatac agcgaaggat
    70201 cgcgtgcgcg ccagctttcg gcgatcacat cccagtacaa cggcgggatc gccagttgcg
    70261 tcaggatctc ttcatctttt accgcgcgat ccaccacctc aaggcacatc tgatgcagtt
    70321 ccgcagtcgg tttttcgatc tgctcttcaa tctggcgcag agtaaaacgg taagcccgac
    70381 tctcatccca atagatttca ttgtcgatga tatgaaagtc gaaaccgttg tcagcggcga
    70441 tctggtccag atcccgtcgc acaggaacgt tgtgtctcag catgattagc ctccccaatg
    70501 cccacgggcg ctggaagaac gaccatagcc gccgcgagaa acggtagacg cttttttggt
    70561 ggtaaagccg cccgaagagt aagactcttt tttgccagaa ccggagcgcc aagagtaatc
    70621 accagaagtg ctgcgccaga ctgggcgcga agcaaaagag gaaccgccgc tgttataaac
    70681 aaacggctca tcgcgatctt tacgaataac ccggcttaac aaaaatccag atattaccgg
    70741 gatccaactc tgttcaacgt tgtcgtagta acaattttca tacttagact gacagttctg
    70801 ctgagtcata ttcttcggaa catcggcata aaacgccgct ttggcgttat tccagccacg
    70861 cgcgcaaatg tcggcattat taccgtcatc aatacaatcc tgcactgtcg agtaaaacgt
    70921 tccgtcgccg tcgttatcga catcgctact ttcactccat cctttgacta ttaaaaaaac
    70981 ggcaccgccc attagcacca acgttaagta tttttgtgcg tagcgattgc ggcgcggctc
    71041 aaaaggatta tgcggtcttc caatgcgact gatcgctccg tgaccaattt tactgttatt
    71101 tcttgatttg cgttttctgg ccatgatcgt ccattaccat gtcatacaag ctgcgttaaa
    71161 aataccgcca gccagcgcgg cggttcccat aaacattcct gctgcggtat tgtgattaat
    71221 aattttttcg cttaatgcgg gcatataaag tctgacacca gcaaaaacga gtaactgaat
    71281 caccagtgca atcccgcccc aggcgaaata gtctggaata ctcaccgcat tgattgccgc
    71341 actggataat gggataacgt aacctaacaa tgtaccactg aacgccagtg aagccgcggt
    71401 attattgttt ttgattaact gccattcgtt gtgcggtgta attttagaat agataaaaag
    71461 aaaaataatc accatagcca cgccaataaa aaaataggcg ctaaaggcaa gaagtgaatc
    71521 cagtatatgc attgtaaacg tccttgtaat taaccaataa tatgaagtga tgtcagcggg
    71581 atatcgacac ccaacgcgcg cgaaaataac cactctggct cgccgagatc gttaaaagat
    71641 tcctcaccat ttaacagcaa atattcgtaa gtatcttcgg ttacttgccg ctggtagccc
    71701 atggtaaaat tatggacctc ccatttggca tggttttgat tttctacttt ttccagcatg
    71761 tagacgggtt cgatatttcc cggttcttcg ctgttaaaaa atcgctgcca gcgtttttcc
    71821 tgccagttta agatcattgc ccccatcact ttggcgttga tcgcctcgcg ccagtgactc
    71881 tctttactga taccgtagct ctcttcatag acaaagagct tgatatcatc aatgtcatca
    71941 atatcttcgc cgccggtggt attgatttgc agaaattcat cgcctgaagt gtagtagcgg
    72001 aaaatctgac taccgccgcc cagatcgatg tggctgacag cggctaccgt aaattcctca
    72061 cccggcagcg caatcagcaa ttcatcttcc agcaaacgaa acgctaacgt gtcgagcgta
    72121 aagccactat tgagatgaag tcccagcgga ccgcgagcga tagcaggctt attatccttg
    72181 ccaaacagac gctggaaaaa accagacata ttttatcctt aaaatagttg ccagcctttt
    72241 agcgaggctg gcaaaaaaat tactcgccct gttggcgttg cagtcttgct aatacatcct
    72301 gggcgctact tttattgcta ccgccaattc cggcttccgc cagcttttca tcaagatcgc
    72361 gaccgtctgc gactttttcc aactgtgcag cagcatccag gcgagcctga cgttcggcct
    72421 gacgcgtttg caggcgtttt aaggattctg ccgctgtcga aacgctggaa gatgcgccaa
    72481 cggtagaggt tgttacagcc tgttgtgcac gctgcatggc ttcagtggct ttaacgactt
    72541 ccatttgctg ctcaaactga gcgatacgct gcgctgtcgc tgtaactgct ttttccacgc
    72601 catcacggga gacttccaga ttcgacaaca cttgctcttc agcggtaatg agattctcaa
    72661 ggcgcgcgat ttcttcagca acttcgttaa tcaacgacgg attaacgttc ttgcttaacg
    72721 cttccagcgc acgggcttcc agactggctt tgcgctcacg taaatctttc agcttatcgt
    72781 gacttaattt cacccgcgcc agcagatcaa cgcgagattt tccggcttta tcgagttcag
    72841 ctttagcgtc acgaatatgc tgttccagca tgcgcacgcc ctgggtttct tcaatggatt
    72901 cttccgcctg ggagataaac gatttcccca gcgtaaataa actttttaaa attcccataa
    72961 atacccgtcc ttgtaattag tgtgaatatt cttctgtaat ttcagccaga tccaatgcgt
    73021 tatctaccag cgaggttatc tcaagcagga tatcttcaag agaagattta agcgataacg
    73081 ccccgaaaac aatgtaatac tcttcctgtt gcacgctgga gattcctacc gatgacaacg
    73141 gcatcatctt ctgatttctt aataagaagg tattaaattc atctggattg ctgatactac
    73201 ttaccggaca aataaaggtt tcgataatca tttggcggga agtaaaaaga atattgattt
    73261 gcaaatcgcc ataatcattc attttaataa ttaatgcgct ctcgctattt gtcacatcaa
    73321 tattttgttc aggtacagtt tgcagcgccg tcgccagcgc caacggattc catgtcatat
    73381 attattccat atagatttag tttaaatatt aataaaatga atatttgcag tacgtaatta
    73441 tcttaccagc tatagacaaa aaaaaaccat ccaaatctgg atggcttttc ataattcaga
    73501 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
    73561 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
    73621 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaggcgt ccggtcattt
    73681 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
    73741 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
    73801 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
    73861 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
    73921 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
    73981 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
    74041 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
    74101 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
    74161 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
    74221 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
    74281 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
    74341 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
    74401 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
    74461 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
    74521 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
    74581 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
    74641 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
    74701 tttgcgctcg tccatattaa ccgcttcgac tcgaacttcc acgcgatcgc ccaggcgata
    74761 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
    74821 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
    74881 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
    74941 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
    75001 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
    75061 tgtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatatt taatggcgcg
    75121 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
    75181 tgccagaccg aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgtag
    75241 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
    75301 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
    75361 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
    75421 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
    75481 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
    75541 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
    75601 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
    75661 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
    75721 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
    75781 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg agcacaggcc
    75841 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtacc
    75901 acgattacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
    75961 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
    76021 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
    76081 gcccgctttt gcttcttccg gcacttcttc tttcagcccg gcaacctgtt gctcaacagc
    76141 ctgcggccag atgtacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
    76201 gcccatatta tcgccaagga cttcgacgat tttacccacc gctttggtac ggcgagtcgg
    76261 gcgttgggtc agttcaacaa ccaccacaaa gcccatccgc gcgcccatga tctgatcggg
    76321 cgggattaag atatcgaagc tcagacggct gtcgtcagga accacaaagc cgacgcccgc
    76381 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
    76441 cgcttcacga cgacctttac ggtcagcgcc cagcggctga gccagcacct gatcgccatg
    76501 aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
    76561 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
    76621 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
    76681 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
    76741 ctcatcacgg ctggccggtt tttcacgttt agttaaatgt tcgaggataa attcccgact
    76801 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
    76861 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
    76921 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
    76981 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
    77041 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
    77101 atcgcactcg ccgatttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
    77161 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
    77221 acgtcagtca cttcagaaat acttgtcatc cgcccttctg gcaatgacgc catgtagatc
    77281 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
    77341 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
    77401 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatagctt
    77461 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
    77521 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
    77581 gagatgatat cgatcggcac accagtcagc tcttcaatac gcttgatgta gttcagcgcc
    77641 gcctgcggca gaccgctacg atctttcacg ccgaaggtgg attcagacca gcccggcatg
    77701 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtcgtcact
    77761 tcacgaccat ccggcatacg gtaagccaca cagagtttca cctctttcag gccatccaga
    77821 acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
    77881 gcaacggtgt ccagccagcc ggtacgacga cgacgccccg tagttgcgcc gaattcgtta
    77941 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
    78001 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
    78061 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
    78121 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
    78181 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
    78241 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
    78301 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
    78361 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
    78421 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
    78481 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
    78541 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
    78601 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
    78661 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
    78721 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
    78781 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
    78841 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
    78901 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
    78961 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
    79021 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
    79081 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
    79141 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
    79201 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
    79261 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
    79321 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
    79381 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
    79441 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
    79501 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
    79561 ggtcggcagg ttgatctgct taattcgcac atcgacaacc tcaataccca gcgccgccat
    79621 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
    79681 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
    79741 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatatc
    79801 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
    79861 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
    79921 gatgcgccat ttgatgtaag agtcgacgat caggtctttc ttctctttgg tcacaaagcg
    79981 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
    80041 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
    80101 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
    80161 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
    80221 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
    80281 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
    80341 cgcagcagat tgctggtacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
    80401 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
    80461 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
    80521 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
    80581 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
    80641 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
    80701 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agcagcctgg
    80761 aagttgacgt ccagcagcgt gatacccatg tcatacggac ggatagtctc ttccagttcg
    80821 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
    80881 ttaccgataa ccccgcgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
    80941 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
    81001 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
    81061 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
    81121 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
    81181 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
    81241 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
    81301 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
    81361 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
    81421 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
    81481 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
    81541 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
    81601 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
    81661 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgcc cttcctgcgg
    81721 tggcagacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
    81781 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
    81841 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
    81901 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
    81961 ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
    82021 ctcttgtaac gtggctttaa atgctgcaac cagatcgtgc ggcaggtggc gaataaaccc
    82081 tacggtatct gccaacacgg tttcaccgac atctgcaacg tcaatacgcc gcaacgtcgg
    82141 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
    82201 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
    82261 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc gcgactgtat
    82321 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
    82381 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
    82441 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
    82501 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
    82561 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
    82621 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
    82681 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
    82741 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
    82801 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
    82861 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
    82921 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
    82981 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
    83041 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcagc aggatcacga
    83101 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
    83161 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
    83221 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
    83281 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
    83341 acttcgtcac gcgcctgttc tggtttttca ctatcaagcc agtgaacccc ttcccaacca
    83401 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    83461 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    83521 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    83581 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    83641 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    83701 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    83761 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    83821 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    83881 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    83941 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    84001 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    84061 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    84121 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    84181 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    84241 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    84301 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    84361 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    84421 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    84481 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    84541 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    84601 taatgccgat ttttcttcgg cagaaacttt tagccgcaac ggaatcagca gcggctgggc
    84661 gcaaacgggc gcttcacccg gcgtcaattg tgcctgacgt agccaacgtt ctgccactgg
    84721 caaggataaa agtgaaatgt tgccatcgcg ctccagcaac gcacagtcgg aatggacgat
    84781 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    84841 cgcttttggt ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    84901 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    84961 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    85021 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    85081 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    85141 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    85201 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    85261 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    85321 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    85381 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    85441 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    85501 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    85561 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    85621 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    85681 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    85741 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    85801 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    85861 gattttactg gtggcatgac gcgccagcgc cagcgccagc tcgtcttttt tgataccgca
    85921 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    85981 tgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    86041 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    86101 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg ccgtttgtgc
    86161 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    86221 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    86281 gttgttgctg ataaaaccgg tttcgaccag tactgatggg atatccggcg aacgcagaac
    86341 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    86401 gatcatactg gtcgctacat cataccctac ccgttgggaa tgaccgaact gtaaatccag
    86461 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcac ccgccccacc
    86521 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    86581 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    86641 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcactgc gccccatcac
    86701 cgagataaag taatccccgt cacgggttaa aacgccctta aacatcggat cgtcattgag
    86761 caaagtacgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    86821 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    86881 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    86941 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    87001 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggcggaggag gaggtggcgg
    87061 cgcatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    87121 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    87181 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    87241 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    87301 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    87361 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    87421 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    87481 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    87541 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acaccagtac cttgttgtgg
    87601 ccactccacc aggcagatgg catcgttggc aaaataatcg cgaatcccca taaactccag
    87661 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    87721 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    87781 taaaaagccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    87841 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    87901 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    87961 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    88021 gtacgtcagc tgcagcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    88081 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    88141 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    88201 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    88261 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    88321 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    88381 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    88441 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    88501 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    88561 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgtagcgccg
    88621 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata attaccagcc
    88681 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt tagccagtga gaaagttgtt
    88741 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccaactatcc agccccagtg
    88801 agtcaaaatg cagttgtccg gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    88861 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    88921 cagccagcag gccggaaggg atatcaaccg ccgcaatcgg cgcaggatgg gaattagcgt
    88981 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    89041 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgcctc
    89101 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    89161 cctgggccag cagcgtgacc tcaatgccga ccgctgtggc cagccgcgcg accacgtagc
    89221 catcgccgcc gttattacca tgaccgcaca gcaccagcca atggcgggcg tcaggatacg
    89281 ccgaacgaca cacctggaat gcggcttcgc cagcgcgaag catcagctca tagagtgtaa
    89341 gccccagcgc atctgccgcc tcacgttctc cgcggcggat atcgtcggcg tgccagacgg
    89401 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    89461 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    89521 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    89581 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    89641 atgagttatt gcccggtacg ctgcgcgtga tcagcgtgcg gatgaattac cttcctgcta
    89701 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    89761 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    89821 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    89881 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    89941 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    90001 atcaaccagt cgaggaagga tgcggtaaat gcgtggcctg tatgacgatt tgcccgaccg
    90061 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    90121 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    90181 gcgatgactg ccagcttatc tgcccgtgga atcgctattc acaactcacc acagaagagg
    90241 atttcagccc gcgtaagccg ctacacgcac cggaactcat tgagttattc gcctggagcg
    90301 aagagaagtt tttaaaagtc acggaaggat cggcgattcg tcgtattggt cacctgcgtt
    90361 ggctgcgtaa tatcgccgta gcattaggca atgccccttg ggatgaaacg attttgacag
    90421 cgctggaaag tcgtaaaggt gagcacccac ttctcgatga gcacatagcg tgggcgattg
    90481 cgcagcaaat agagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    90541 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    90601 gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa atcgtcaagt gatcgactcg
    90661 acaaaacgga atcttaattt ttaacttatc tatcaatagg ttaaaaaaat actattcacc
    90721 gtgcgaagtt tctttgaaat taagattaca gcctgaggct gtggataact ctgtttacaa
    90781 gagtttgtca gagacaacga aaaccagcaa taacgcatcg catcgctgtg gatattttat
    90841 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    90901 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgctttgca gcacttttca
    90961 aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
    91021 taccaactga gctattcccg cgtactactt aattttgctt tcgtaatttt cgcatttctg
    91081 cgtcgttacg ggaggcgcat tatacgagaa atctttccct ctgcaacccc ccaaaaaagt
    91141 tttttttgcg tttttatctc aagtgctgaa attatcagca agacgttcaa tttagcgtca
    91201 aaatcacgcc gccgcaagtg gattccggcg cttacgtgac caggaaaaat tttacagctt
    91261 gataaaatgc tcgcggtagt aagccagctc cgccaccgat tcacggatat catccatcgc
    91321 ctgatgcgtc ccctgcttgg taaaaccatc cagaatttcc ggcttccagc ggcgcgccag
    91381 ctctttcagg gtgctgacat cgagataacg gtagtggaag taggcttcca gctccggcat
    91441 gtatttaaac aggaaacgac ggtcctgacc gatgctgtta ccgcaaatcg gcgattttcc
    91501 cgcaggcacc cactgtttta aaaattcgag cgttgccagt tcagcttccc gatcgcccat
    91561 cgtgctcgct ttcacgcgct ctaccagccc gctggcggta tgggtgcgca cgttccagtc
    91621 atccatcagc gccagctgtt catcagactg gtgtactgca atggtcggcc cttctgccag
    91681 aatattcagg ttggcatcgg tcaccagcgt ggcaatctca ataatgcgat cgcgctcggg
    91741 atccagaccg gtcatctcaa gatcgatcca aatcaggttg ttttcattgg cactcatgct
    91801 attttccacc cattcacagt gactatattg atccacaatt gcgtgtatca tagatgtttt
    91861 gcccatcagg ggcgaccagg agtctgtacg attgagtaaa aataaactct ccaaaggcca
    91921 gcagcgccgc gtgaacgcca atcaccagcg tcgtcttaaa acgtctaagg agaagcccga
    91981 ctacgacgac aatctgtttg gcgagcctga tgaaggtatc gtcatcagcc gctttggtat
    92041 gcacgctgat gtggaatccg ccgatggcga cgttcaccgc tgcaatattc gccgtaccat
    92101 ccgttcgctg gtaaccggcg accgcgtagt ctggcgtccg ggtaaaccgg cggcggaagg
    92161 cgtaaatgtc aaagggatcg tggaagcggt gcatgagcgt acctcggtgt tgacgcgtcc
    92221 ggatttctac gacggcgtaa aacctattgc cgccaacatc gaccagattg tcattgtctc
    92281 cgccattttg ccggagctgt cgctcaatat tatcgaccgt tacctggtgg cctgcgaaac
    92341 cttgcagatt gagccgatta ttgtgctcaa caagatagac ctgctggacg acgaaggcat
    92401 ggcgttcgtc aacgagcaga tggatatcta ccgcaatatc ggttatcgcg tattgatggt
    92461 ttccagccat actcaggatg ggctaaaacc gctggaagag gcgttgaccg ggcgcatcag
    92521 catttttgcc gggcagtctg gcgtcggcaa atccagcctg ctgaatgcgt tgctggggct
    92581 gcaaaaagag atcctgacca acgatgtctc tgacaactcg gggctcggtc agcacaccac
    92641 gaccgccgct cggctgtatc acttcccgca cggcggtgat gtgattgatt cccctggtgt
    92701 gcgtgagttc ggcctctggc acctggagcc ggaacaaatc actcagggct ttgtcgaatt
    92761 ccatgactat ttaggtctgt gtaaatatcg cgattgcaaa cacgataccg atccgggctg
    92821 cgctatccgg gaagcggttg aggaagggaa aatcgcggaa acccgtttcg aaaactatca
    92881 ccgtattctg gaaagcatgg cgcaggtaaa aacgcgtaaa aacttttctg atacggatga
    92941 ctgacaatta agctaaccgt cgttagaatc gtcccctttt tttcaggatc cggcatgttg
    93001 gccggatcag gaacgacaaa acaatggcct ggaggctacc ttgttaaatt catttaaact
    93061 ttcgctacag tacattctgc cgaaactatg gcttactcgc ctggcgggtt ggggcgcaag
    93121 caagcgggca ggatggctga caaaactggt tatcgatctg ttcgttaaat actacaaggt
    93181 cgacatgaaa gaggcgcaaa agccggacac cgccagctac cgcaccttta acgaattctt
    93241 tgtccgtccg ctgcgtgacg aagtacgccc aatcgatacc gatccgaatg tactggtcat
    93301 gcctgccgat ggcgttatca gccagttggg taaaatcgaa gaagataaaa tcctgcaagc
    93361 caaaggccac aactacagcc tcgaagccct gctggcaggc aactatctga tggcggacct
    93421 gttccgcaac ggtacgtttg tgaccactta cctctccccg cgtgactacc atcgcgtaca
    93481 catgccgtgc aacggtattc tgcgtgagat gatctacgta ccgggcgatc tcttctccgt
    93541 taaccatctc acggctcaga acgtgccgaa tctgtttgcc cgtaacgaac gcgtgatttg
    93601 ccttttcgat accgaatttg gcccaatggc gcagattctg gtcggagcga cgattgttgg
    93661 cagcattgag acggtctggg cgggcaccat tacgccgccg cgcgaaggta tcatcaagcg
    93721 ctggacctgg cctgccgggg aaaacgacgg ttctgtggca ctgctgaaag gccaggaaat
    93781 gggtcgcttt aaactcggtt ccaccgttat caacctgttt gcaccgggta aagtgaatct
    93841 ggttgagcaa ctggaaagcc tgtctgtcac gaaaattggc cagccgctgg cagtatctac
    93901 cgaaaccttt gttacgccag acgctgaacc ggccccgctt cctgctgaag agatcgaagc
    93961 agaacacgac gccagcccat tggttgacga taaaaaagac caggtctaat ccatcaaagg
    94021 aaacgctgac gtgcgcctga ttatcacttt tctgatggcc tggtgcctca gttggggggc
    94081 gtacgccgcg acggcccccg atagcaaaca aatctcacaa gaactggagc aggcaaaagc
    94141 ggcgaaaccc gcgcagccgg aagtcgtaga ggcgctccag tccgccttaa atgcgcttga
    94201 ggaacgaaaa ggttcccttg agcgcatcaa gcaatatcaa gaagtcattg ataattaccc
    94261 gaaactttcc gctactctgc gcgcacaatt aaacaacatg cgtgacgaac cgcgcagcgt
    94321 gtcgccgggg atgtctaccg acgcgctgaa tcaggaaatt ctccaggtca gcagccagtt
    94381 gctggataaa agccgtcagg cccagcaaga gcaggagcgc gcccgcgaga ttgccgattc
    94441 gctgaatcaa ctgccgcaac agcaaaccga cgcccgccgt cagttaaatg agatcgagcg
    94501 ccgcctggga acgcttactg gcaatacccc gctcaatcag gcacaaaatt tcgcgttgca
    94561 gtctgactcc gcacgcctga aagcgctcgt tgatgaactg gaactggcgc agctgtctgc
    94621 caataaccgt caggaattag cgcgcttacg ctcagagctg gcggaaaaag agagccagca
    94681 actggatgcg tatttgcagg ccttacgtaa tcagttgaac agccaacgtc agcttgaggc
    94741 ggagcgggcg ctggaaagta ccgaattgct ggcagaaaac agcgccgatt tgccgaaaga
    94801 catcgtcgcg caattcaaaa ttaaccgcga actatcggcg gcactgaatc aacaggcgca
    94861 gcggatggat ctcgttgcct cgcaacagcg ccaggccacc agccagacgt tacaggttcg
    94921 gcaggcgctg aatacgctgc gtgaacagtc gcaatggctg ggatcgtcca acctgctcgg
    94981 cgaagcgttg cgggcgcagg tggcacggct gccggaaatg ccgaaaccac aacagcttga
    95041 taccgaaatg gcgcagttgc gtgtgcaacg gttacgttat gaggatctgc ttaataaaca
    95101 gccgctgcta cggcaaattc atcaggccga cggtcagccg ctgaccgccg agcaaaaccg
    95161 tattctggaa gcacagctac gcactcagcg tgagttgctg aactcattgt tgcagggtgg
    95221 cgacacgcta ctgctggaac tgaccaagct gaaagtctcc aacgggcaac ttgaggatgc
    95281 gctgaaagag gtgaatgaag cgacgcatcg ctatctgttc tggacctctg acgtgcgccc
    95341 gatgaccatc gcctggccac tggaaatcgc ccaggatctg cgtcgtctca tttcgctgga
    95401 caccttcagt cagttgggca aagccagtgt gatgatgctg accagcaaag agacgatttt
    95461 gccgctgttt ggcgcgttga ttctggtcgg ttgcagtatt tactcgcgcc gctatttcac
    95521 ccgttttctt gaacgttcgg cggcgaaagt cggcaaagtg actcaggatc acttctggct
    95581 gacgttgcgc actcttttct ggtcgattct cgtcgcgtca ccgctgccgg tgctgtggat
    95641 gacgctgggt tacggcttgc gcgaggcgtg gccttatccg ctggcggtcg cgattggcga
    95701 tggcgtaacg gccaccgtgc cgctgctgtg ggtagtgatg atttgcgcca cctttgcccg
    95761 cccgaacggc ttgtttatcg ctcattttgg ctggccgcgc gaacgtgttt cccgtgggat
    95821 gcgctactac ctgatgagca tcgggcttat tgtgccgctg attatggcgc tgatgatgtt
    95881 cgataacctc gacgaccgtg aattctccgg ttcgctggga cggctttgct ttatcctcat
    95941 ttgcggtgcg ctggcggtgg tcaccctcag cctgaaaaag gccgggatcc cactgtatct
    96001 caacaaagag ggcagcggcg acaacattac caaccatatg ctgtggaaca tgatgattgg
    96061 cgcgccgttg gttgccattc tggcgtcggc tgtgggttat ctggcaacgg cacaggcgct
    96121 gttagcgagg cttgaaacct cggttgccat ctggttcctg ctactggtgg tttatcacgt
    96181 tatccgccgc tggatgctga tccagcgtcg caggctggcg tttgaccggg cgaagcatcg
    96241 ccgggcagag atgttagcgc aacgcgcgcg tggcgaagag gaagcgcatc atcacagtag
    96301 cccggaaggg gcaattgaag tcgatgaaag cgaagtcgat ctcgatgcca tcagtgcgca
    96361 atccttgcgg ctggtgcgct caattttgat gttgatcgcc ctgctttcgg tcattgtgct
    96421 gtggtcagaa atccattccg ctttcggctt cctcgaaaat atttctctgt gggatgtcac
    96481 ttccactgta cagggcgtag aaagcctgga gccgattacc ctcggtgcag tgctaattgc
    96541 cattctggta tttatcatca ccacgcagct ggtgcgcaac ctgccagcgc tgctggaact
    96601 ggcgattttg cagcacctgg atttaactcc gggtacgggt tacgccatca ccaccatcac
    96661 caaatatctg ctgatgctga ttggcgggct ggtcggcttc tcaatgattg gtattgagtg
    96721 gtcgaaattg cagtggctgg ttgccgcgct cggtgttggt ctcggttttg gtttgcagga
    96781 aattttcgcc aactttatct ctggtctgat tatcctgttc gaaaaaccga ttcgcattgg
    96841 cgatacggtg acaattcgcg atctcaccgg tagcgtgacg aaaattaaca cccgcgccac
    96901 caccatcagc gactgggacc gtaaagagat aatcgtgccg aacaaggcgt ttattaccga
    96961 gcagtttatc aactggtcgc tctctgactc ggtcacgcgc gtggtgttga cgataccggc
    97021 ccctgccgat gccaatagcg aagaagtgac ggaaatcctg ctcaccgcag cgcgtcgctg
    97081 ctcgctggtg atcgacaacc cggcaccgga agtcttcctg gtggatctgc aacaggggat
    97141 tcagattttc gagctgcgta tttacgccgc tgagatgggt caccgtatgc cgctacgcca
    97201 tgagatccac cagctgattc tggcaggctt ccacgcccac ggtatcgata tgccattccc
    97261 gcccttccag atgcgtctgg aaagcctcaa cggtaaacaa acggggagaa cgctgacgtc
    97321 tgcgggcaaa ggtcgtcagg cgggaagttt gtaagtttgt gtgccggatg aaccaatccg
    97381 gctcacaaac aaaactgata gcgcagaaaa aactgccaca gccagtagcc gaaaatagcc
    97441 agcgcggtgg cgtacataaa ccagccgcgt ttttttcgcc acaaagcgaa ccagagaaaa
    97501 gggaaaaaga gcggtaacgt cattatcaca ccaacatcac gggtatcgtc ttcttcgaga
    97561 tacatcaaca catcgcagac gttgttaatc ccgcgtccat caatcatcca ctctttgtcc
    97621 agcgcggaag tgatggttat ccagaggaac gccacaataa accagatgca gcacaagaca
    97681 aacatgcgcg cactcataag gtaaccggtc gcttaatttc gctatagcgc atacgaaaca
    97741 aatcgccgta tcccactaat tccccgcgaa aaaccagaca tcggataaga taagcgcaat
    97801 aaatatccgg caggtttccc caccgtttac catcgcgatc tttgagggtg ttaatggtgc
    97861 tacccaccac catcgccact ttacgggtgt ggcaggcatc gacaaaagag agaatcgtct
    97921 gctctatttc cgctacatcc ccggcagaaa attgcagcgc cctgatttca tcttcggtta
    97981 ttgccgggtc ccggctggtg tcatccataa catttcctgt ctttacgccc tatcgtcagg
    98041 cagacgatag ggcatcgggt tattccaccg cccactgcgg gttttttgcc actctgctgc
    98101 aatagttctg gtaaatcgcc atcgctaaca gcgagaagaa gacagggccg ccaatcatcc
    98161 acaatgtgct gtcccagtct ccggcttcaa ccacaggctg aataatggtg aagacgttcg
    98221 caaatgtcac caccagtacg acaaccactg tcgcaatcat tgccgacaaa tgcgttttga
    98281 aaatcacaaa tggtctgtcg agatcctgac gtgctttaaa gaatgggaaa gccagcgcga
    98341 ggaacaggta aggaagcgtc atagacacgt tcgccatcag cgtcagcttg ttaaagaacg
    98401 cggatgcggt accgccacca aacgaaacca gcaggatgaa gacagtaacc aacccgcact
    98461 gcatccacat ggcgatagac ggcatcccca tcgcattcag gcgcgtcatc ggttccggcc
    98521 acaacgcttt cggcgtcccc tggatgatgg ctttcagcgg tgaatagcaa agcgtaaaga
    98581 acgcaccggt ataagcgagg aacatcgaaa gtccggtaat acgcgcaaac catacgccca
    98641 gcgacaatga cgcttcaggt gacaaatgca gtgcgttacc cagcgtcacc ccgaggctct
    98701 tcatcagcac ataggtaata ttgccaaggt taacggaacc attacttaat acctgctgcc
    98761 agtttgtgct gacaccccat aaaaatattg ccagcgaata accgattgaa ataacaatag
    98821 cggcaaaaac aatacctttg gcaaagttct tttctggatt ttccgtttta tcgaccagac
    98881 caccaaccgc ttcaattccg ccataggcaa aaatagcaaa taccacaaac gatagcattg
    98941 ccagaccgga ctgataacct gggttcggtg atgcaaggaa attaatatcc tgcgcgaaat
    99001 gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca
    99061 ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa
    99121 cgacggtgac cagaatcatc catgccactg ccagcagacc aaccacctgc gtaggctcca
    99181 gtccagcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta
    99241 cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg
    99301 taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccgcctt
    99361 cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata
    99421 acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg
    99481 ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca
    99541 tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc
    99601 ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata
    99661 cctggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat
    99721 atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa
    99781 ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa
    99841 catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa
    99901 ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg
    99961 gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gtcaattcat ggaaaccatt
   100021 cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt
   100081 actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc
   100141 tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcagcg tgtcgcggtc
   100201 ttcttcggta tcagcaacat tgctcaaatc cagtttcgct gcgacttccc gcagttgcgt
   100261 tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag
   100321 gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa
   100381 ccggtacata tcatagtgcg gtcgatacca ctccagcata gtgaactcag ggttgtgata
   100441 acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc
   100501 aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg
   100561 cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt
   100621 cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcggcaa agaaacgacg
   100681 gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca
   100741 ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg
   100801 caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa
   100861 aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc
   100921 ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt
   100981 gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct
   101041 gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc
   101101 gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag
   101161 gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc
   101221 gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac
   101281 tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc
   101341 gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt
   101401 cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg
   101461 ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa
   101521 ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt
   101581 catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct
   101641 aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc
   101701 ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac
   101761 atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa
   101821 ggctgccgcg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat
   101881 tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt
   101941 ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc
   102001 acgccgcgtg gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat
   102061 gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa
   102121 gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag
   102181 aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg
   102241 cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtctg
   102301 gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt
   102361 gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc
   102421 gaaaactggg cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg ttgcggtatc
   102481 taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc
   102541 ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc
   102601 attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt
   102661 aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc
   102721 aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac
   102781 agcgacgtga agattactac gctgccgcca gctaaacgtg tttacggtgg cgaagcggat
   102841 gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa
   102901 cctaaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt
   102961 ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga
   103021 caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg
   103081 cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac
   103141 cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga
   103201 tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg
   103261 caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt
   103321 ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc
   103381 agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga
   103441 ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac
   103501 tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca
   103561 gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg
   103621 agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga
   103681 aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt
   103741 ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg
   103801 cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg
   103861 cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca
   103921 ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg
   103981 tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt
   104041 aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt
   104101 atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg
   104161 gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc
   104221 attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt
   104281 atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac
   104341 ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc
   104401 gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt
   104461 tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc
   104521 aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc
   104581 ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg
   104641 cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca
   104701 gcaaaacatt tacgggcgtg cttggtggcg aggctattgc tcgaggggaa atcaagttaa
   104761 gcgatcccac aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac
   104821 tattacatct cgcaacctac actgctggcg gcctgccatt gcaggtgccg gatgaggtga
   104881 aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg gcgccaggaa
   104941 cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt
   105001 ctggtttgag ttttgagcag gcgatgcaaa cacgtgtctt ccagccactc aaactcaacc
   105061 atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag
   105121 gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga aacttatggt gtgaagtcga
   105181 ccattgaaga tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca
   105241 aaacacttca gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
   105301 atcagggcct gggctgggaa atgctggact ggccggtaaa tcctgacagc atcattaacg
   105361 gcagtggcaa taaaattgca ctggcagcac accccgtaaa agcgattacg cccccaactc
   105421 ctgcagtacg cgcatcatgg gtacataaaa caggggcaac cggcggattt ggtagctatg
   105481 tcgcgtttat tccagaaaaa gagctgggta tagtgatgct ggctaacaaa aactatccca
   105541 atccagcgag agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa aatttcatcg
   105601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
   105661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
   105721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg taacagtagt gaataatttc
   105781 gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
   105841 cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc
   105901 attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac
   105961 tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
   106021 ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac
   106081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
   106141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
   106201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
   106261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
   106321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
   106381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
   106441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
   106501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
   106561 aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
   106621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
   106681 tacctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
   106741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
   106801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
   106861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
   106921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
   106981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
   107041 gtgagcagcg tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
   107101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
   107161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
   107221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
   107281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
   107341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
   107401 ttgtcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
   107461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
   107521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
   107581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
   107641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
   107701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
   107761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
   107821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
   107881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
   107941 gaaaggcaat ccggacgatc ctcttttgca tcaggtactt acctcgcaag atgagtttgt
   108001 cgtcgcgtcc ggattctcca ccgacccgct ggaagaacag cacagcgtag tgcctggttt
   108061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
   108121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
   108181 gcagactgct cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
   108241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
   108301 catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg
   108361 tatcaccgag gcgctggttg aacgctttgc ccgttctacg ctgcaaatct tgctggtgaa
   108421 tcacatcaat catgccaatg aggtagatga aacgttccgt caggcgatgg ctaaattgcg
   108481 ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga acgataacgc
   108541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtaatgc cgtattacct
   108601 gcatgtgctc gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg
   108661 gcagattatg cgtgagttgc tgacactggt gtcgggttat ctggtgccga aactggcgcg
   108721 agaaatcggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagtaact
   108781 gattaaacag ggaagttaaa cattagcctg atgcgctacg cttatcaggc ctacattgtg
   108841 cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt acgccgcatc
   108901 cggcaagagc aacaaacgca agtacagcaa cctgtcatta aactgggaaa tttaatcttt
   108961 gcccgttgca taacctttaa ttattcatat acattgctgt tgttactctt tatatagcca
   109021 acaccaataa ttaacctttc ttttctgttt atatagagtg agtacggcta tttatgttat
   109081 tttaacaact attactacca a
//
LOCUS       DAPHDX010000002        95507 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPHDX010000002 DAPHDX010000000
VERSION     DAPHDX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.002"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            12..530
                     /locus_tag="RH058_000114"
     CDS             12..530
                     /locus_tag="RH058_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV3135761.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            546..1085
                     /locus_tag="RH058_000115"
     CDS             546..1085
                     /locus_tag="RH058_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV3135762.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="RH058_000116"
     CDS             complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="RH058_000116"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV3135763.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="RH058_000117"
     CDS             complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="RH058_000117"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV3135764.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4454..5824
                     /gene="xseA"
                     /locus_tag="RH058_000118"
     CDS             4454..5824
                     /gene="xseA"
                     /locus_tag="RH058_000118"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV3135765.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5821..6036)
                     /locus_tag="RH058_000119"
     CDS             complement(5821..6036)
                     /locus_tag="RH058_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV3135766.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6105..7577)
                     /gene="der"
                     /locus_tag="RH058_000120"
     CDS             complement(6105..7577)
                     /gene="der"
                     /locus_tag="RH058_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV3135767.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7695..8873)
                     /gene="bamB"
                     /locus_tag="RH058_000121"
     CDS             complement(7695..8873)
                     /gene="bamB"
                     /locus_tag="RH058_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV3135768.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8884..9504)
                     /gene="yfgM"
                     /locus_tag="RH058_000122"
     CDS             complement(8884..9504)
                     /gene="yfgM"
                     /locus_tag="RH058_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV3135769.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9522..10796)
                     /gene="hisS"
                     /locus_tag="RH058_000123"
     CDS             complement(9522..10796)
                     /gene="hisS"
                     /locus_tag="RH058_000123"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV3135770.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10907..12025)
                     /gene="ispG"
                     /locus_tag="RH058_000124"
                     /gene_synonym="gcpE"
     CDS             complement(10907..12025)
                     /gene="ispG"
                     /locus_tag="RH058_000124"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV3135771.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12052..13065)
                     /gene="rodZ"
                     /locus_tag="RH058_000125"
     CDS             complement(12052..13065)
                     /gene="rodZ"
                     /locus_tag="RH058_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV3135772.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13350..14504)
                     /gene="trmG/rlmN"
                     /locus_tag="RH058_000126"
     CDS             complement(13350..14504)
                     /gene="trmG/rlmN"
                     /locus_tag="RH058_000126"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV3135773.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14654..15085)
                     /gene="ndk"
                     /locus_tag="RH058_000127"
     CDS             complement(14654..15085)
                     /gene="ndk"
                     /locus_tag="RH058_000127"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV3135774.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15234..17546)
                     /gene="pbpC"
                     /locus_tag="RH058_000128"
     CDS             complement(15234..17546)
                     /gene="pbpC"
                     /locus_tag="RH058_000128"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV3135775.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17547..22508)
                     /gene="yfhM"
                     /locus_tag="RH058_000129"
     CDS             complement(17547..22508)
                     /gene="yfhM"
                     /locus_tag="RH058_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV3135776.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22715..23560
                     /gene="sseA"
                     /locus_tag="RH058_000130"
     CDS             22715..23560
                     /gene="sseA"
                     /locus_tag="RH058_000130"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV3135777.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24053..24829)
                     /gene="sseB"
                     /locus_tag="RH058_000131"
     CDS             complement(24053..24829)
                     /gene="sseB"
                     /locus_tag="RH058_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV3135778.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24971..26254)
                     /gene="pepB"
                     /locus_tag="RH058_000132"
     CDS             complement(24971..26254)
                     /gene="pepB"
                     /locus_tag="RH058_000132"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV3135779.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26432..26632)
                     /gene="iscX"
                     /locus_tag="RH058_000133"
     CDS             complement(26432..26632)
                     /gene="iscX"
                     /locus_tag="RH058_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV3135780.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26644..26979)
                     /gene="fdx"
                     /locus_tag="RH058_000134"
     CDS             complement(26644..26979)
                     /gene="fdx"
                     /locus_tag="RH058_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV3135781.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26981..28831)
                     /gene="hscA"
                     /locus_tag="RH058_000135"
     CDS             complement(26981..28831)
                     /gene="hscA"
                     /locus_tag="RH058_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV3135782.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28848..29363)
                     /gene="hscB"
                     /locus_tag="RH058_000136"
     CDS             complement(28848..29363)
                     /gene="hscB"
                     /locus_tag="RH058_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV3135783.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29459..29782)
                     /gene="iscA"
                     /locus_tag="RH058_000137"
     CDS             complement(29459..29782)
                     /gene="iscA"
                     /locus_tag="RH058_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV3135784.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29799..30185)
                     /gene="iscU"
                     /locus_tag="RH058_000138"
     CDS             complement(29799..30185)
                     /gene="iscU"
                     /locus_tag="RH058_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV3135785.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30213..31427)
                     /gene="iscS"
                     /locus_tag="RH058_000139"
     CDS             complement(30213..31427)
                     /gene="iscS"
                     /locus_tag="RH058_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV3135786.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31539..32027)
                     /gene="iscR"
                     /locus_tag="RH058_000140"
     CDS             complement(31539..32027)
                     /gene="iscR"
                     /locus_tag="RH058_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV3135787.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32328..33065)
                     /gene="trmJ"
                     /locus_tag="RH058_000141"
     CDS             complement(32328..33065)
                     /gene="trmJ"
                     /locus_tag="RH058_000141"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV3135788.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33184..33987
                     /gene="suhB"
                     /locus_tag="RH058_000142"
     CDS             33184..33987
                     /gene="suhB"
                     /locus_tag="RH058_000142"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV3135789.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34132..34986
                     /locus_tag="RH058_000143"
     CDS             34132..34986
                     /locus_tag="RH058_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3135790.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     YPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35177..36457
                     /gene="csiE"
                     /locus_tag="RH058_000144"
     CDS             35177..36457
                     /gene="csiE"
                     /locus_tag="RH058_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV3135791.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISATGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36449..37588)
                     /locus_tag="RH058_000145"
     CDS             complement(36449..37588)
                     /locus_tag="RH058_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV3135792.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37748..38638)
                     /gene="hcaR"
                     /locus_tag="RH058_000146"
     CDS             complement(37748..38638)
                     /gene="hcaR"
                     /locus_tag="RH058_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV3135793.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38774..40135
                     /locus_tag="RH058_000147"
     CDS             38774..40135
                     /locus_tag="RH058_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV3135794.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40132..40650
                     /locus_tag="RH058_000148"
     CDS             40132..40650
                     /locus_tag="RH058_000148"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV3135795.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40650..40970
                     /locus_tag="RH058_000149"
     CDS             40650..40970
                     /locus_tag="RH058_000149"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV3135796.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40967..41779
                     /gene="hcaB"
                     /locus_tag="RH058_000150"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40967..41779
                     /gene="hcaB"
                     /locus_tag="RH058_000150"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV3135797.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41789..42991
                     /locus_tag="RH058_000151"
     CDS             41789..42991
                     /locus_tag="RH058_000151"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV3135798.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43088..43510
                     /locus_tag="RH058_000152"
     CDS             43088..43510
                     /locus_tag="RH058_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV3135799.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43558..44430)
                     /locus_tag="RH058_000153"
     CDS             complement(43558..44430)
                     /locus_tag="RH058_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV3135800.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLLALAPG
                     ESTTSEMSLRVALL"
     gene            complement(44442..45536)
                     /locus_tag="RH058_000154"
     CDS             complement(44442..45536)
                     /locus_tag="RH058_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV3135801.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45569..46567)
                     /locus_tag="RH058_000155"
     CDS             complement(45569..46567)
                     /locus_tag="RH058_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3135802.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGFLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46592..48103)
                     /locus_tag="RH058_000156"
     CDS             complement(46592..48103)
                     /locus_tag="RH058_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3135803.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48126..49109)
                     /locus_tag="RH058_000157"
     CDS             complement(48126..49109)
                     /locus_tag="RH058_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV3135804.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49206..52487)
                     /locus_tag="RH058_000158"
     CDS             complement(49206..52487)
                     /locus_tag="RH058_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV3135805.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQSCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52605..53798
                     /locus_tag="RH058_000159"
     CDS             52605..53798
                     /locus_tag="RH058_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV3135806.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53862..55115)
                     /gene="glyA"
                     /locus_tag="RH058_000160"
     CDS             complement(53862..55115)
                     /gene="glyA"
                     /locus_tag="RH058_000160"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV3135807.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55443..56633
                     /gene="hmpA"
                     /locus_tag="RH058_000161"
     CDS             55443..56633
                     /gene="hmpA"
                     /locus_tag="RH058_000161"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV3135808.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56678..57016)
                     /gene="glnB"
                     /locus_tag="RH058_000162"
     CDS             complement(56678..57016)
                     /gene="glnB"
                     /locus_tag="RH058_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV3135809.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57077..58411)
                     /gene="glrR"
                     /locus_tag="RH058_000163"
     CDS             complement(57077..58411)
                     /gene="glrR"
                     /locus_tag="RH058_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV3135810.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58401..59114)
                     /gene="qseG"
                     /locus_tag="RH058_000164"
     CDS             complement(58401..59114)
                     /gene="qseG"
                     /locus_tag="RH058_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV3135811.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59279..60706)
                     /gene="qseE"
                     /locus_tag="RH058_000165"
     CDS             complement(59279..60706)
                     /gene="qseE"
                     /locus_tag="RH058_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV3135812.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61282..65169)
                     /gene="purL"
                     /locus_tag="RH058_000166"
                     /gene_synonym="purI"
     CDS             complement(61282..65169)
                     /gene="purL"
                     /locus_tag="RH058_000166"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV3135813.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAAGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65427..66983
                     /gene="mltF"
                     /locus_tag="RH058_000167"
     CDS             65427..66983
                     /gene="mltF"
                     /locus_tag="RH058_000167"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV3135814.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66980..67516)
                     /gene="tadA"
                     /locus_tag="RH058_000168"
     CDS             complement(66980..67516)
                     /gene="tadA"
                     /locus_tag="RH058_000168"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV3135815.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(67541..68176)
                     /gene="yfhb"
                     /locus_tag="RH058_000169"
     CDS             complement(67541..68176)
                     /gene="yfhb"
                     /locus_tag="RH058_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV3135816.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68385..69233
                     /locus_tag="RH058_000170"
     CDS             68385..69233
                     /locus_tag="RH058_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV3135817.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69289..69549
                     /gene="yfhL"
                     /locus_tag="RH058_000171"
     CDS             69289..69549
                     /gene="yfhL"
                     /locus_tag="RH058_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV3135818.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69743..69823)
                     /gene="shoB"
                     /locus_tag="RH058_000172"
     CDS             complement(69743..69823)
                     /gene="shoB"
                     /locus_tag="RH058_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV3135819.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70243..70623)
                     /locus_tag="RH058_000173"
     CDS             complement(70243..70623)
                     /locus_tag="RH058_000173"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV3135820.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70623..71354)
                     /gene="pdxJ"
                     /locus_tag="RH058_000174"
     CDS             complement(70623..71354)
                     /gene="pdxJ"
                     /locus_tag="RH058_000174"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV3135821.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71366..72094)
                     /gene="recO"
                     /locus_tag="RH058_000175"
     CDS             complement(71366..72094)
                     /gene="recO"
                     /locus_tag="RH058_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV3135822.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72106..73011)
                     /gene="era"
                     /locus_tag="RH058_000176"
     CDS             complement(72106..73011)
                     /gene="era"
                     /locus_tag="RH058_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV3135823.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73008..73688)
                     /gene="rnc"
                     /locus_tag="RH058_000177"
     CDS             complement(73008..73688)
                     /gene="rnc"
                     /locus_tag="RH058_000177"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV3135824.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73961..74935)
                     /gene="lepB"
                     /locus_tag="RH058_000178"
     CDS             complement(73961..74935)
                     /gene="lepB"
                     /locus_tag="RH058_000178"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV3135825.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(74951..76750)
                     /gene="lepA"
                     /locus_tag="RH058_000179"
     CDS             complement(74951..76750)
                     /gene="lepA"
                     /locus_tag="RH058_000179"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV3135826.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76948..77427)
                     /gene="rseC"
                     /locus_tag="RH058_000180"
     CDS             complement(76948..77427)
                     /gene="rseC"
                     /locus_tag="RH058_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV3135827.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77424..78380)
                     /gene="rseB"
                     /locus_tag="RH058_000181"
     CDS             complement(77424..78380)
                     /gene="rseB"
                     /locus_tag="RH058_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV3135828.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78380..79030)
                     /gene="rseA"
                     /locus_tag="RH058_000182"
     CDS             complement(78380..79030)
                     /gene="rseA"
                     /locus_tag="RH058_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV3135829.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79063..79638)
                     /gene="rpoE"
                     /locus_tag="RH058_000183"
     CDS             complement(79063..79638)
                     /gene="rpoE"
                     /locus_tag="RH058_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV3135830.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79635..79790)
                     /gene="rseD"
                     /locus_tag="RH058_000184"
     CDS             complement(79635..79790)
                     /gene="rseD"
                     /locus_tag="RH058_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV3135831.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80046..81668
                     /gene="nadB"
                     /locus_tag="RH058_000185"
     CDS             80046..81668
                     /gene="nadB"
                     /locus_tag="RH058_000185"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV3135832.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPIPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81653..82390)
                     /gene="trmN"
                     /locus_tag="RH058_000186"
     CDS             complement(81653..82390)
                     /gene="trmN"
                     /locus_tag="RH058_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV3135833.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82522..83856
                     /gene="srmB"
                     /locus_tag="RH058_000187"
     CDS             82522..83856
                     /gene="srmB"
                     /locus_tag="RH058_000187"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV3135834.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKVKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(83889..84770)
                     /locus_tag="RH058_000188"
     CDS             complement(83889..84770)
                     /locus_tag="RH058_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3135835.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
                     EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVGNELASGELIELPFGEQTQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFVAG"
     gene            84873..85460
                     /gene="eamB"
                     /locus_tag="RH058_000189"
     CDS             84873..85460
                     /gene="eamB"
                     /locus_tag="RH058_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV3135836.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(85515..85898)
                     /gene="grcA"
                     /locus_tag="RH058_000190"
     CDS             complement(85515..85898)
                     /gene="grcA"
                     /locus_tag="RH058_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV3135837.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86203..86892
                     /gene="ung"
                     /locus_tag="RH058_000191"
     CDS             86203..86892
                     /gene="ung"
                     /locus_tag="RH058_000191"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV3135838.1"
                     /translation="MANELTWHDVLAEEKKQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(86940..87977)
                     /locus_tag="RH058_000192"
     CDS             complement(86940..87977)
                     /locus_tag="RH058_000192"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV3135839.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88184..88603
                     /gene="trxC"
                     /locus_tag="RH058_000193"
     CDS             88184..88603
                     /gene="trxC"
                     /locus_tag="RH058_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV3135840.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88672..89370
                     /gene="tapT"
                     /locus_tag="RH058_000194"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88672..89370
                     /gene="tapT"
                     /locus_tag="RH058_000194"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV3135841.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGELFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89402..92062
                     /gene="pat"
                     /locus_tag="RH058_000195"
     CDS             89402..92062
                     /gene="pat"
                     /locus_tag="RH058_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV3135842.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92176..93531
                     /gene="pssA"
                     /locus_tag="RH058_000196"
     CDS             92176..93531
                     /gene="pssA"
                     /locus_tag="RH058_000196"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV3135843.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGIPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93577..93900
                     /locus_tag="RH058_000197"
     CDS             93577..93900
                     /locus_tag="RH058_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV3135844.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(93897..95195)
                     /gene="kgtP"
                     /locus_tag="RH058_000198"
     CDS             complement(93897..95195)
                     /gene="kgtP"
                     /locus_tag="RH058_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV3135845.1"
                     /translation="MVESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22770 a        26049 c        24023 g        22665 t
ORIGIN      
        1 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
       61 tcactctggc aggatgccag tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg
      121 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
      181 aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
      241 gcgcagtcgc tggcggtgtt atcggccaca acgtcggttc tggcagcaat tccggaacga
      301 cggcaggggc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
      361 aaaccttagt ggaaggtgtt tctttaacat ataaggaagg caccaaagtg tatacctcta
      421 cccaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacaacgt
      481 ataacgaaac gcgtattcag ccaaatacta aatgtcctga aaagagctaa taatcaggag
      541 gagtcatgaa gaaagttttt ctttgcgcca tcttggcctc cttaagctat ccggctatcg
      601 cctcatcatt gcaggatcaa ctctctgctg tagcagaagc tgaacagcaa ggtaaaaatg
      661 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
      721 agcaacgtcg cgcaaatgcc caggccgccg ctaacaaaag agcggcaacg gcagcagcaa
      781 ataagaaagc tcgtcaggat aaactggacg ccgaagcctc tgcggacaaa aaacgcgatc
      841 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg gcgctggcga
      901 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggaactg aagcacaaag
      961 ctgcgcaaac cgatgtggtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg
     1021 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa caaatcggac agctggttta
     1081 attaatcgat gttagtaact tcaagcctat gattcttgaa gataaaaaac cctctgtagt
     1141 aacagagggt tttgttcatt catagtgcag ggattaaaat cattcccact caatggtagc
     1201 tggcggcttg ccgctgatgt catacaccac gcgggaaata ccgttcactt cattgataat
     1261 gcggttggaa acgcgaccga ggaaatcgta cggcagatgc gcccagtgtg cggtcataaa
     1321 gtcgatggtt tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat
     1381 tacgccaacg gaacgtaccg gcaggaacac agtgaacgcc tggctgactt tgtcgtacag
     1441 gtccgcttta cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacagta
     1501 ctctttcttc acttcaccca gcacacgaac gccaaggcct ggtcccggga acgggtgacg
     1561 gtacagcatg tcgtacggca ggcccagctc cagaccaatc ttacgcactt cgtctttgaa
     1621 cagctctttc agcggttcaa ccaggcccat cttcatctct ttcggcaggc cgcccacgtt
     1681 gtggtgagat ttgatgacgt gtgctttacc ggttgcagaa gccgcagatt cgataacgtc
     1741 agggtagatg gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc
     1801 gaatacttca acgaaaacgc gcccgatgat tttacgtttt gcttccggat cgttttcgcc
     1861 agccagcgct gacaggaagc gatcttctgc cggtacgtga acaatgttaa gaccaaagtg
     1921 atcgccaaac atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac
     1981 gaatacgcaa gtcaggtttt taccgatagc gcggtgcagc agcattgcgg ttacggagga
     2041 atccacacca ccagagaggc cgaggatgac tttatcgtcg ccgacctgct cgcggatgcg
     2101 agctacagca tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc
     2161 acgcacaaaa cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa
     2221 ctgtacgcca tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt
     2281 gctggctacg gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca
     2341 gacatcgagc agcggttttc cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc
     2401 gctgtcgttt acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac
     2461 gtgaccgccc aactgcattg ccatggtctg catgccatag caaacgccga ataccggtac
     2521 gcctgcttca aagacatact gcggcgcacg cgggctgttt tcttcggtgg tgctttccgg
     2581 gccgccggaa agaataatgc cgcttggatt gaagtcacga atttgtgctt ctgtcacatc
     2641 ccacgcccac agttcgcagt aaacacccag ctcacgcacg cggcgcgcaa ccagttgagt
     2701 gtactgagaa ccgaagtcca gaatgaggat gcgatgctta tgaatgtttt ccgtcattga
     2761 cgcttattcc gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa
     2821 gagaatcagg agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg
     2881 tggctttcct gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg
     2941 tcgatagtac cacagccggt cagacccata caggagcgca ggccgcccat ctgctggtga
     3001 atgatctctt tcaggcgacc tttataagct acgcgacctt cgataccttc cggcaccagt
     3061 ttgtcggcag cgttatcgct ctggaaataa cggtcagagg aacctttgga catcgcgccc
     3121 agggaaccca taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc
     3181 ggagattctt cagtacccgc cagcatggaa cctaccatca ccgcgcttgc gccagcggcg
     3241 atagctttgg cgatgtcgcc ggagaagcga ataccaccat ctgcgataac cggaataccg
     3301 gtgccttcca gggcttcaac tgcgtcagca acggcagtga tctgcggaac acctacgcca
     3361 gtaacgatac gagtcgtaca gatagaacca gggccgatac cgactttaac cgcgctgcaa
     3421 ccagcttctg ccaaagcgcg tgcacctgca gctgttgcca cgttgccgcc gatgatttgc
     3481 agatccggat atttagcacg ggtttcacgg atacgctgca aaacgccttc tgagtgaccg
     3541 tgggaggagt cgatcagcag aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct
     3601 tcgttacccg cacctgcgcc aactgcagca ccaacacgca gacggccttg ctcgtcttta
     3661 caggcgttcg gtttacgttc cgctttctgg aagtctttca cggtgatcat gccgatcagg
     3721 tggaattcgt catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc
     3781 accacttcac gggcttcacc ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa
     3841 acgctaaccg gctggttcag gtcggtaaca aaacgcacgt cacgaccggt gataataccc
     3901 accagttcgt tttcttcggt aacgaccgga tagcccgcaa aaccgttacg ctcagtcagt
     3961 tctttcactt cgcgcagcgt cgtggttggc agaacagtct gcggatcagt caccacacca
     4021 gattcgtgtt ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg
     4081 tggataaagc cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg
     4141 gtatccattg ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg
     4201 ctgaggtcag cagtattcgg cagaacggta gaatgagcag gaacgaggag aacgtcgtca
     4261 aacgtcagag cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat
     4321 aaatattgcc gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa
     4381 atgcttgcta tccccgaagg gcgggttact atcgactgaa taacctgctg atttagaatt
     4441 tgatctcgct cacatgttac cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa
     4501 tcaaacggtt cgtctgctgc ttgagcatga gatgggacag gtttggatca gcggcgaaat
     4561 ctctaatttc acacaaccgg cttccggtca ctggtacttt acactcaaag acgacaccgc
     4621 ccaggtacgc tgcgcgatgt tccgcaacag caaccgccgg gtgaccttcc gtccacagca
     4681 tggacaacaa gttttagttc gcgccaatat tacgctctac gagccgcgcg gtgactacca
     4741 gataatcgtt gagagtatgc agccggccgg tgaagggctg ctgcaacaga agtacgaaca
     4801 gctcaaagcg aagttgcagg ctgaaggttt gttcgatcag caatacaaaa aaccacttcc
     4861 ctcccctgcg cattgcgttg gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat
     4921 tttgcatgtg ttaaaacgtc gcgatccttc tctaccggtg atcatctacc ccaccgccgt
     4981 tcagggcgat gacgcaccgg ggcaaattgt tcgcgccatt gaactggcga atcagcgcaa
     5041 tgagtgcgac gtgttgatcg ttgggcgcgg cggcggttcg ctggaagatt tatggagttt
     5101 taacgacgaa cgcgtagcgc gggcgatttt tgccagccgc attccggtcg tcagcgccgt
     5161 cgggcatgag acggatgtga ccattgccga ttttgttgcc gatctgcgtg cgccaacgcc
     5221 gtctgccgcc gctgaagtag tgagccgtaa tcagcaagag ttactgcgcc aggtgcaatc
     5281 gacccgtcaa cggctggaga tggcgatgga ttattatctc gccaaccgca cgcgtcgctt
     5341 tacgcagatc catcaccgat tacagcaaca gcatccgcag ctccggctgg cacgccagca
     5401 aaccatgctt gagcgcctgc aaaagcggat gagctttgcg ctggaaaatc aacttaagcg
     5461 taccgggcaa cagcagcagc ggttaacaca gcggctgaat cagcaaaatc cacagccgaa
     5521 gattcatcgc gcgcaaacgc gcattcagca actggaatat cgtttagcag aaaccctgcg
     5581 cgtacagctt agcgccacgc gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt
     5641 aagcccactg tcaacgctgg cgcgtggata cagcgttact actgctactg acggcaatgt
     5701 actgaaaaaa gtgaagcaag ttaaagcggg tgaaatgcta accacacgtc tggaagacgg
     5761 ctggatagaa agtgaagtta aaaacatcca gccagtaaaa aaatcgcgta aaaaggtgca
     5821 ttaagccagc acgaattcaa cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa
     5881 gtaatctact gcaccgcagg cttttaacac ctgcaacggt tgatggcagt ctggacaaag
     5941 agctttcatt tcaataattt ccccgcagga tgggcaacgc gcatggccgt tatcctgatc
     6001 aagcacatgc tgacattgtg gacaatgcag ttccatatga attcctccgg tagccatccc
     6061 tctacattca tagagggaat ggcagataaa atacttacgg ataattattt atttttcttg
     6121 atgtgcttca tcagacgctt acgtttacgc atctgggttg gcgtcagggt gttacgcttg
     6181 ttcgcatacg ggttttcccc ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc
     6241 agcgatttgc ggaagtagtt catcaagtag cgcttgtagg aatcaggcag gtctttcacc
     6301 tgattaccgt gaatcaccac aatcggcggg ttataaccac cagcgtgggc atatttcagc
     6361 ttcacacgac gaccgcgtac cagcggcggt tggtgatctt caacagccat cgtcatgatg
     6421 cgcgtcagca tagaggtccc cacacgacgg gtggagctgt catacgcttc acgtactgat
     6481 tcaaacaagt taccaacacc actgccgtgc aaggcagaga taaagtgcac acgagcaaaa
     6541 tcgataaagc ccagacggaa gtccagcgtt tctttcacct gctctttcac ttcctggctc
     6601 aggccatccc acttattcac cacaatgaca agtgagcgcc cactattgag aataaagccc
     6661 agcagcgaga gatcctgatc ggaaatacct tcgcgcgcat caatcactaa catcaccacg
     6721 ttggcatctt caatggcctg caacgttttg attacggaga atttctctac agcatcggtg
     6781 attttgccgc gtttacgtac gccagcggtg tcaatgagca catactcacg tccatcgcgt
     6841 tccattggaa tgtagatact gtcacgcgtc gtgccaggca tgtcataaac aacaacgcgc
     6901 tcttcaccaa gaatacggtt agtgagtgta gacttaccta cgttcggacg acccacaatc
     6961 gccagtttga tcggcagact ttgcgggtcg aagtcgtctt cctcttcttc ttcgccgttc
     7021 tcttccgctt caaattgcgc ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg
     7081 agatcttcca tccacggcag cagcacatgc tccagcagac ttaatacgcc acgaccgtga
     7141 gacgcggcga tcgggtagat ttcacctaag ccaagcgagt agaaatcaac cactgcctga
     7201 tcgggatcca gaccgtcagt tttgtttgcc accaggaagg tcggtttttc acgggagcgc
     7261 agatgtttgg caatcgcttc atctgccggc atcaggcccg cgcgcgcatc caccataaac
     7321 agtacgacgt ccgcttcttc aatcgccagc agcgactgtt ccgccatgcg ggtttctaca
     7381 ccgtcttctg tgccatcaat cccgccggta tcaatacaga taaactcacg gccttcaatt
     7441 tccgcacgac cgtacttacg gtcacgagtc agacccggga aatccgcaac cagcgcatct
     7501 cgggtgcgag ttagacggtt aaataacgtg gattttccta cgttagggcg cccgacaagc
     7561 gcgaccacag gtaccatgtt taaagcctca tttttataaa tcatcagaca acgcacgcta
     7621 tattcgcgtc gttgttaaaa acaggaaaac ggcccctgtc caggagccgt tttcaaagtg
     7681 aacgacagag acgattaacg tgtaatagag tacacggttc cgtcttttgc ctggatcagc
     7741 agtttgccgt cagcggcaac cggttcagtc tggaaaccgg aactatcaac tttttgctgg
     7801 gcaacgaaac gaccatcttc gacgttaatc cagtgcagat aaccttcact gtcacctacc
     7861 accaggttgc cattatacag caccggagaa gtcagcaggc gatgcaacag atcgctttgt
     7921 gtccacagcg taacgccgcc atcaatggtc aacgccatca cccggtcatt ttgatcgacc
     7981 agatagatgc gattgccgtc gacgatgaaa tcattcaccg aacccagttc gcgtttccac
     8041 ataatctgac cactgcgcag atcaagcgcc gtcaggttac cattataggc cagcgcgaaa
     8101 acaacgccgt taacaacgac gggagtcgtg tcaacatcgc tcagacggtc aatttcggta
     8161 gaaccggtcg cctgggaaat acgctgctgc caaatcatct ggccctgttc catcagcact
     8221 gcgctgacgc gaccattatc gccacccacg acggccgcac caaaagccgt tgccggcgca
     8281 gactcgccac gcaaagagag cgaaggcata tcgaggttaa ctgtccattt gacagcgccg
     8341 tcagcctcgt tcagcgcttg taactgaccg ttactggtgt ggattaacac cagaccgtcg
     8401 ctgaccaccg ggcgcgaaag tgcttcaccc gcgactttag tttgccatgc cacagtacca
     8461 tcgctggtat tcagcgcgta aacctgcgcc ttttcgctgc caatgtagac atgcccacca
     8521 gacacggtca gaccgccaga aagtaatgca ggctctttag agaaccagcc atctttctcg
     8581 gccaggctga cagaccagat ttctttgcca tcatccgcat tcagcgcttt taccaaacca
     8641 gcgcggtccg ctgcatagac aacgttgtcc gccagtgccg gatgaagatt ggaatagaag
     8701 ttgccaatgc cgctaccaac ggaagtgctc cacgccgtgg tcggcgtaaa ctggttttca
     8761 acggttggca atggggacat ctttaccaca tcttcttcgc tgttaaacag cgaacagccg
     8821 cttaaaaggg taacggaaag cagtcctggc agcagtaatt tacgcaattg catcgggtcc
     8881 ctctcagatg gacaaattat taattttcat ctgcatcatt tcgctcagtg ccggagtaac
     8941 atcgcttttc acgcctgctt cccatgcact acgcgcacct tgcttatcac ctttgctcag
     9001 caatgcttca ccacgcaggt cggcaacaat ggcagcccac ccttcacctt tgatggtatc
     9061 aagggttttc agcgcggcat cagcctgctt gagctgtacc tgaacgcgag caagacgcag
     9121 atttatcacg gctttgagat tttcatcgct cgtgtctgcc agcccctgtt gtaactgggc
     9181 ggcagctttc tctagttcat ttttgtcaac aaattgctgc gcaagttcca aagaagccag
     9241 cgcaccataa gtatttttat tttcagcagc aaatttttcc gccgccggga tgctatccgg
     9301 tttgccttcg ctcactgcgg taaccgcatt ttgataggca agagaagcgg agcgtgcaga
     9361 atcaacctga tggctgttcc agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac
     9421 cccaacagcc agtgctttgc cattttcagc aaaaaagcgt ttaaccgctt ctacctggtc
     9481 gttttcgttc tcgtaaattt ccacgctgtc cttctccttc cttaacccag taacgtgcgc
     9541 aaatgcgcgg ctacgctatc ctgcgcaact gccgtttgct caccagagcg caaatccttc
     9601 actactgctg tgccgttagc cacttcagac tcacccagca ccacagcaac gcgggcaccc
     9661 catttatcag cacgggcaaa ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat
     9721 ttcacgcccg gtaattcatc acgcagacgc tcagctaatg ccatagccgc agattgtgta
     9781 tcagcacctg aagccaccag gtatatatcg acaacaggat cggctttaaa ttccggatta
     9841 acggcctgaa ctaacaatac aagacgttcg aggcccatcg caaaaccgac tgccggtgtt
     9901 gcacgaccgc ccagttgttc caccagaccg tcataacgac cgcctgcaca cacggtgccc
     9961 tgggagccga gactgttagt cacccactcg aaaacggtac ggttatagta atccagacca
    10021 cgcaccagac gctggtttac ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga
    10081 ccggcaaaat gctcacgaga ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg
    10141 agaagcgcct gcacttccgg attttttgaa tccagcacgc gcagcgggtt agtgtacatg
    10201 cggcgtttgc agtcttcgtc cagcttttct ttatgctgct caaggaatgc caccagcgca
    10261 tcgcggtaat tggcgcgtgc ttccagcgaa ccgatagaat tcagctcaag agttacgtgt
    10321 tcggaaatac ccagcgcgcg ccaccagcgg gcggtgagca taatcagttc agcgtcgata
    10381 tccggacctt gcagaccgaa aacttcgcag cccaactgat ggaactgacg ataacgccct
    10441 ttctgcggac gctcgtgacg gaacatcggc ccgatatacc acagacgctg ttcctgattg
    10501 tacagaagac catgctcgat gccggcgcgt acacagcccg ccgtcccttc agggcgcaga
    10561 gtcaggctgt cgccattgcg atcctcaaag gtgtacatct ctttttcaac cacgtcggtg
    10621 acttcaccaa tcgcacgttt gaatagcggg gtctgctcta caatcggcaa gcggatttca
    10681 ctgtaaccgt agctgccgag cacgtttttc agtgtgcctt caatgcgctg ccagatggcc
    10741 gtttcgccag gcaggtaatc gttcatgccg cgaatggctt gaatgttttt tgccacgttt
    10801 attctctttc tgaatataaa aatgaaccct caacgcttcc ctcaatgttt ggggagccat
    10861 gcgggttcaa tcatacacgg gaagcgaggc gcttcccatc acgttattat ttttcgacct
    10921 gctgaacgtc aattcgacgc gcttcgtcca gctgactggc tttcgcacga atgcgtgctt
    10981 ccagctggtc gatcatatcg ttgttgtcca gacggtcttt gcgcacgcca tcttcataga
    11041 ggccgctttt cttgttgccg ccggtgacgc cgagtgtaga aaccagcgcc tcacctgggc
    11101 cattcaccac gcagccgata atcgaaacgt ccatcggagt gatgatatct tccaggcgtt
    11161 gctccagcgc gttaaccgta ccgataacat caaactcctg acgcgaacag gtcggacagg
    11221 cgatgaagtt gatacctcgc gaacggatac gcagcgattt caaaatatcg aaaccgactt
    11281 tgatctcttc gaccggatcg gccgccagtg atacgcgcag cgtgtcgccg atgccttcag
    11341 acagcagcag acctaaacca atggcggatt ttactgcccc gctgcgcgcg ccacccgctt
    11401 cggtgatccc cagatgcagc ggctgatcga tttgttttgc cagcaaacga taagactcaa
    11461 cagcgaggaa gacgtcagac gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat
    11521 cgagatgatc aacatggcgc atggcggatt ccagcaacgc ctgcggcgtc ggttcgccat
    11581 acttttcttg cagatctttt tccagcgatc cggcgttaac gccaatgcgg atcggaatgt
    11641 ttttatcgcg cgcacagtca actaccatgc gaatacgctc ttcattaccg atattgccag
    11701 ggttaatacg cagacaatcg acgccgtatt ccgctacttt cagcgcaatg cgatagtcga
    11761 agtggatgtc agccaccagc ggcacgttaa cccgctgttt gatgagtttg aacgcttctg
    11821 ccgcgtccat cgtcggtacg gagacacgga cgatatcagc gccaacgcgt tccagcgcct
    11881 tgatttgatt gaccgttgct tcgacatctg tcgtacgcgt attggtcatg gactgtacgg
    11941 cgatgggagc accatcgcca atcggcacat tcccaacgta aatacgtgtt gattttctac
    12001 gttgaattgg agcctggtta tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc
    12061 cggtgattgt tcggcattga gggtcagacg cgcaacctgg ttagttctga taaaacgact
    12121 cagatcgaca ggtttccctt gatactggat ctgtactgcg gctggcgcac caattttcag
    12181 tttgtacggt gcctggccgg ttaagttcaa attaccgtct ttacgctgca taccgctaaa
    12241 caatttttta ccggtagcat cagtaacctc cagccagcaa tcggcagtaa agttcatcac
    12301 cagcgcattc ggatcagccg ccggcgtagt cacgcctgcc tgatcggttg gcaacggcgc
    12361 agcgccatct ggcgttgttg ttgccgttgg tgccggggtc gcggcggtat caacatttgc
    12421 ctgcgaaggc gaaacaaccg cattctgttg cggatcaaca gctggtgctg gcgcagttac
    12481 ggcaggtgtt tgcgtgttgg ttgcggtagt atccacagac gctggcggcg tggacgttgt
    12541 agccgggtct gtagttgtcg acgtatttaa cggaacactc tgcccctgct cgctattact
    12601 gctcagttcc gccgaagatt gatcggccat agtggtgatc tcttcctgct gagctttgtg
    12661 gtcttgccac caccaggcac cgctcaggcc gataaccaca aacaacacca gccaggtgaa
    12721 ggtcatcagc cagccgtcac gttttttgcg gcgtttaccg agggaaaaac tctgcatcgg
    12781 cgcaactttc gcagcccgta gtggagcctg cttttccagc cctggcagca gttcttcttc
    12841 tggaatatgt accagacgcg cataagagcg gatatatccg cgcaggaatg ttgaagcaag
    12901 atcggcgggt gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg
    12961 ctcggcaacg gcctgctgac taagtcctag ttgttcgcga gcattacgca ggcgagcgcc
    13021 ggtagtaagt gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt
    13081 ttaaatgaga attgagatgc cggtgaacca tcatgctcac ccacaccgcg aaacatccgt
    13141 taagttaacc gttatcatac agtataagac cgtctgtgtg ctcgtgacaa agcccacatt
    13201 cactaaggct aacttactgt tgcatcgtta catactgcct taaagtcagc aaaaacgcac
    13261 cgttaatatt gaccagacaa ttgcaactta atacttcatt cataaaagat tacgccacgg
    13321 tacataaagt aaccgtggcg taatggctat cagaccgctt taatgtcgat ggcttcaccc
    13381 tgcatccgtt tacgcagggt acgtttcgta cggtcgataa catcgcccgc caactgacca
    13441 caggcagcat cgatatcatc accacgggtt ttacgcacaa tagtggtgaa accgtagctc
    13501 atcagcactt ttgagaaacg gtcgatacgg ctgttcgagc tgcgtccata cggcgcgccc
    13561 gggaaggggt tccacgggat caggttgatc ttacacggcg tatctttcag cagttccgcc
    13621 agttggtgcg cgtgttcagt gccgtcgtta acgtggtcaa gcatcacata ctcaatagtg
    13681 acacggccct gattggcgtt ggatttctcc agataacggc gcaccgcagc aaggaacgtt
    13741 tcgatattgt actttttgtt gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg
    13801 tgcagggaaa ttgccagtgc aacgtcgatc atatcgccca gtttatccag cgccggaact
    13861 acaccggaag tagaaagcgt gacgcgacgt ttagacaggc caaaaccgaa atcatcaagc
    13921 atgatttcca tcgccggaac gacgttgttc aggttgagca gcggctcgcc catgcccatc
    13981 atcactacgt tagtgattgg gcgctgaccg gtgacttttg ctgcgccgac gattttcgcc
    14041 gcacgccaca cctggccaat aatttccgat acccgcaggt tgcggttaaa gccctgctgg
    14101 gcggtggaac agaatttaca ctccagcgca caccccacct gcgaagagac gcagagcgtg
    14161 gcacggtcgt cttccgggat atacaccgtt tcgacgcgct gatcgccaac ggcgatcgcc
    14221 catttaatgg tgccgtcgga tgaacgctgt tcttcaacca cttccggtgc gcggatctcc
    14281 gccacctctt tcagtttgcc gcgcaacact ttgttgatgt cggtcatctc atcaaagttg
    14341 tcgcagcaat agtgatacat ccacttcatc acctgatcgg cgcggaaggg tttttcacct
    14401 aaatctttaa aaaactcccg catctgctga cggttgagat ccagcaggtt gatttttcca
    14461 tctttcgtgg tgacgttttc aggtgtgact aattgttcag acatatgcta ttccggcctc
    14521 gttattacac gttatggccc ctggagggtt gaaaaaagaa acgccccggt gagcggctgc
    14581 tcgtccgggg gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc
    14641 atttacgaaa ttattaacgg gtgcgcgggc acacttcgcc ttcgccaaag aaataagcga
    14701 tttcgcgagc ggcagattcg acggaatcag aaccgtgggt accgttttcg gtcaggctgt
    14761 cagcgtaatc agcgcgcaga gtaccagcca gtgcgtttgc cggattggtc gcgcccagca
    14821 gatcgcggtg acgctgcacg gcgttttcac cttccagcac ggaaaccacg atcgggccag
    14881 aggtcatgaa ttcaaccaga ccatcaaaga acggttttcc atcgtgttca gcataaaagc
    14941 cacgtgcctg ttcaacggtc aggtgcagca ttttggtgcc gacaattttg aaccctgcag
    15001 cttcaaagcg cgcaaagata ttaccaatga cgttttttgc taccgcgttc ggtttgatga
    15061 tggaaaaagt acgttcaata gccattttta cctctgtaaa ttgttctgtt gttgtctgta
    15121 ccagcgtacg agatggcgcg gattataatg agcaacaggg ccgttgacta ttgatgaagg
    15181 taacattttt ttaaaataaa atgagtttta gcaacaatca ggggcaaaac agactattgc
    15241 atgacaaatt tcactgtcgc gatttgcccc acatcatcca tcaccagcaa ttgataatca
    15301 cctttatccg tcaaatgcag ggtcacgttg cgcccgcgtt cagttaacgg ttcgccattc
    15361 agaaaccacc agcgttcacc tgcccctcca cttgattgca acggcaaagt cgcttccgct
    15421 gcgcccggta aacgtttaat aatcgcgcca tcgcgaacgc ctgtcagctg cagagggagc
    15481 ttggcatcgt gaccgtatgg cgggcaaatt gtcgaggccg gtggtaagcg cacagcgcga
    15541 cgttctgatg cgggcagcca gggttccagc ggtagcggcc agacatttat catttcttgt
    15601 cgcgcttgcg ggcaatcagc ggcaacacgt ttgccatttt catccagcca gatggggaaa
    15661 cgaatgccat taatgccttc ctgctccggc agtaatagag ttggcggctg actcccgtcc
    15721 agcagccagg ttgccaggcg gcggcgacag ttaccgtcac cttccggcaa agactgtccg
    15781 cccggccagc agataacgcc acgagtgact gagttcgggc gcgggtcttc cggcagattc
    15841 gcactgcgcg acagtaagat attattgacc tgattcaaca atggtacggc actggcaaag
    15901 ccaaactgac caacaacggg cgtgccgtcc ggtctgccag tccagatccc aatgacatag
    15961 cgagcgttaa ccccaatcgc ccaggcgtca cgatagccat agctggtgcc cgttttccat
    16021 gccagtgggg cgacgcgcgg caaggcacta tccggcaagg gtttcgcttc atcagccata
    16081 atccggcgaa tgatccacgc cgcccccgac gacattaaag gccgttcaag cagcggatcg
    16141 tcaggctgta agcgcaattt gcctgccttg ccgtggcgag caaacgcggt atacgctgcc
    16201 gccatatctt ccagttttgc accagcgccg ccgagaatga gtgaaagatt cggcgcagca
    16261 ccgttgggca aatataacgg caatccaaca ttgcgtaact ttgccgcaaa ccgtttcggt
    16321 ccataggctt ccagcacctg cacagcaggt aagttcagcg agcgcaccag cgcctcgctc
    16381 atgctgatcg ggccatgaaa accgctatca aagttacctg gtcgataatc accggtgcgc
    16441 cgggggacgt cttgcagcag tgatgccggg tggatcaagc cttcatccag cgccagacca
    16501 taaacaaacg gtttgagcac cgatcctggc gatcggatcg cattgaccat atcaacatga
    16561 ccaaagcgtg aatcatcgtt gagatcaacc gatcccaccc agccgcgaac acgcatatcg
    16621 gtatgatcaa ccacgatcat cgccagtgag ctgcgcggtg gcaaccgccc tttccagttt
    16681 tgcgccagtt cttccagacg tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg
    16741 cttttgcttt taccgagcat catgcgcgaa aacagcggtg ccagttgcgg catttgtcgg
    16801 ggtgccagcc agatgggttc ttcccttgac tcttttacct gctcacggga ccacacacct
    16861 tgcacggcca tccgttcgag cactttatta cgcgcggctt cggcacgctc cggccaacga
    16921 tccgggcgaa gacggctggg cgcttgcggc aaaaccgcca gcattgccgc ctcggaatag
    16981 cttaaattcg caggcgactt tccgagataa gcccaacttg ccgcaccgat cccctgcaac
    17041 gtaccgccaa acggagcgcg gttaagatac aaggtcagaa tttcacgctt agacagatgc
    17101 cattccagtt gcaacgcgcg ccagagctgg cgaattttgc cgccaaatgt tttggggtga
    17161 ggatcaagca gacgagcaac ctgcatagtg agcgtgctgc caccggaaat aacccgtccc
    17221 gaagtgagat cttgccatgc tgcgcgcgcc accgagaatg gattcacccc cggatgcttc
    17281 cagaaccagc gatcttcata attgatcagc gcttcaaggt aacgtggaga aacatcttcg
    17341 attgttaccg gataacgcca gatgccgtca gcatcggcga agcgccatag cggcgtacca
    17401 tcctgcgcca cgaccactcg tgcgggattg acttcatgca gcggtagagg ccagagttta
    17461 tctgctcccc acgctgcaag aaaaaggaga aatggcgcag ctgccagcgt tatccagcag
    17521 ccgcgtttgg ttaacaagcg aggcatttac ggtcggacaa tcagcagatc ttcagccgcg
    17581 ccggtcgccc gccattgggg aacatacatt gattccacca tcggttgcgg tacctgatac
    17641 gttcccggcg tcaccgcccg cgccagatac accagcgtta ccggttggta ttcatcaacg
    17701 gcaaccgccg ccacaaagcg atcgtcacgg aactcaatgt gcttaatgct cgcctgctgc
    17761 atctggttca gtaagttttg cacttcgcca ccactttgct ccaggctggc gctaccgttc
    17821 gccagattct gattttccag ttccagaccc gcaggcagca gatccacgac taacgcatcc
    17881 ggcacgctgt tactggcttt tacctgcaac cagaccagca ccagatcgcc gctacgtaac
    17941 gagtccagcg atttgctctt accatcagta ccaagaatat gacgctcgat ttgcagcaca
    18001 ttgttcgccg gtaaaggtgc ggattgcgga taaccgctgg catccatacg caaccataac
    18061 ggctgatcgc cactgtttct cacctgcaag gtggcaagtt gatcgctatt cagattgctg
    18121 tttagcgttt tctcgcctgt cagcggctca gctgagaaag aggtttgcgc ctgccattta
    18181 cccggtaaat cctgaatcgt acgggcagcc aggaacaacg cgttactttc ctgcgtcgat
    18241 agccagcgtt caccaaacgc ctgctgcgaa agtgtgttca gcaaagtgta ctgctcatcg
    18301 ggtagcagtt tattttcttc cagcaaggag agcattaacg cgttgtcgcg cagtggacta
    18361 ccgtaatcac ccagccatat ccgctcatca ctatttctcg gcgttttcag cgccagcaca
    18421 atcgcttctt caccacgcgt tgcatcaccc atggttttca gcgcaacgcc aagttgcagc
    18481 agcggtaaac cagaagcggc atctgcacga tgctcccaga tttcacgcag cgcacccagt
    18541 ggtgcttttt gctgacgggc cagcaccagc gcagcgtaag actgaacggc gaatttactg
    18601 gctttgagat tatccgcgta cgggatcgac atcatgcccg gatcttgtaa atagcgcagc
    18661 agacgctcat taccccggtt aatggcgtct gtcggcacgc tgtaaccctg ttcgcctgcg
    18721 cggaccagga aatccatcac gtaagccgtc agccagtact cttcgtcacc gtttttatcc
    18781 cacagcgcaa agccgccgtt atcacgttgc atttgcagca aacgggaaat gccgatatcg
    18841 accgatgcac ggcgtttctc atcactgtcg cctttgatgc ccaacgcctg cagttgggct
    18901 gcgttggtat aaagtgacgg gaacaggccg ctggcggttt gctcaagaca gccgtacgga
    18961 tacgctttta actctttgat ataacgtgca atgttcagcg gtggtttgcc gctcaacaac
    19021 aattgccctt ccagcgtaac aggcgagaag ttttgcaatc catccgccgg aatcgcccat
    19081 gtctcaccag gctgtaacgc cgtaccgtaa ttaaccgttt gtgccgggaa cgccggacgg
    19141 acgccgattt tccactgctt atgctgatcg gcaacggttt cacccggtaa cgctaacccg
    19201 ctaatggtgg cctgaatttc tccatcgcca taacccggca atgctcgcac cgggataaac
    19261 agcgtagtac gcacacctgg cgctaattca acggctgcgg gtgaatcgct gaccagttca
    19321 agcaaaccac tggcggtcag ggcaacgttc agtttttgcg gtttatcggt aagattagtg
    19381 atatccagcg tcagacgcga ggtatcgcca ctcgccataa agcgcggcat gttcagttca
    19441 gcaatcaccg gtgcggcaac tatcactttg ctttcgttgc taccgaaatc atctgccgtc
    19501 caggcttgcg ccatgacgcg caattcaccg ttaaaatcgc caatcggcag tgtaaccgag
    19561 ccttcgccct gttcgttgag cgttaccggc agcgcctgct gcgcgacaat attgacgtga
    19621 ttgaccggcg gtttaccacc acgtttcagc tcatcaccat cgccaccgaa acgcagagct
    19681 gccagacgcc cctgaccttc aataacctga ccgtaaatat cgtaaatgtc tgcgccatag
    19741 cgtttctgac caaagaacgc ctgccacgga tctggcgtga cgtagtcagt aatattcagc
    19801 acaccactat caacggcaga caccagcaca ttcacctgtt taggcttctc gccattttta
    19861 gtgctggctt taattttcac ggttaatggt tgattgggac gcatttttgc tggtgtttcc
    19921 agcgccagat cgaggcgacg gttttcatcg ccaagcggca gatgcaacac accaaccgcg
    19981 cgttttggcg tcgcggagcg agatttatcg ccaggacgta ccaccagcgt acttaaatac
    20041 agatcatgac gattccaggt tttatcgacc ggaatcgtca gatccagccc ttgagcccga
    20101 acatcaatct cttgccacca cagcggccct tcactggact cgaccatcgc ataaccttta
    20161 cccgccgttg gcgcggcgat atgcaactta atggtgtcgc cagggcgata actggcttta
    20221 tccagtttca gcgtgacacg gtcgggtcgc actgcgccgc taccgtcgct gttgtcctgc
    20281 cagctatagc cagcccagaa acgaacacta ctgaccgctt cattcggcgc tttgacttcc
    20341 agacgataag cgccccactc taccggaaaa ctgaccttgc cggtttcgtc cgctttcaga
    20401 tccagagttt gttcattttc gatcagatct ttttgatcaa actgtgactg ccagccttca
    20461 tcttctgacc agttccagta gtaatcgcgg cgttcgcgaa tcaggcgcac ctgcaagccc
    20521 gacacggctt ttttcacgcc ttgcgcatcg ctataaacga tgtcaaaagc ggcgttactg
    20581 ccttcatcaa caatgggctg tttcaccgtg ctgtcagtac gataatcgta aaccgatttc
    20641 gaggcgaact gcggacggat ccccggcaat gcatcggcag gccagatagc ctgctcagcg
    20701 cggcgcgtca ccgggcgacc gcccgattcc agcaaactac cctggaaaat aacctgtaat
    20761 ggggaatgcg tttccttcca ctggctttct gtagaaactt cgccgcgccc tttatcatcc
    20821 agcgtcaact gaacttcatc cagcgtgcgg gaaagatttt cggcagctat atcgccgaat
    20881 tcaaaaccag gtaaggctga cacagcttca cgcagtgggc gcaggaaaag ttgcccttgc
    20941 aaagtattac cattagcagg tgcaccatac aggtagtacc ccaccacgga gaatttcact
    21001 tcatctttcg gcgttagcgg ggttttctca ccggtcagat tcagcgccat gcgctctggc
    21061 ataaaatctt cgacgtggaa atcccacatc cgatactggt tatcgcccgt gttagcgcga
    21121 atatgccaca taccggttgc cgcattgcta tcgagtggcc aggtaaagtg gtagaggcca
    21181 ttctccggct gactaacgac gctcctgagt acctgcccat cgggtttaat cacgtctaac
    21241 ttgatgggtt gattgggcaa cgctttaccg tctgcatcac gcagcaaacc attgaggatt
    21301 accgtttcac ctgggcgata aagatcgcgt gggccaaaca tgaaaaactg tttgctatag
    21361 cctggcgcgc cagcaatgtt aaattctgct aagtccagcg ccggaagttt taaatcgagt
    21421 agcgttgtct gaccgtcttt acgcgccaac agtaatgccg cgtttttatc attttccagc
    21481 tgcacatgcc cctgagcgtc actggttgcc tgagtcagag tctgcccttt ctcatttaat
    21541 aaagagactt caattccttg ctgggccgcg ccgttttcca gactttgggt aaagatatcc
    21601 agacgattgt gataacggtg agctgaaacg ccgatatcac ttaacgtaaa cagcgtcgcg
    21661 ggattactgt aatcgtaacg tccagcctga ttcatcacag ccagatacac gcccgcctgc
    21721 tgaagcggtt tgatatcgcc cagcggcagc aataattttt cacgggtgtt acgcgcagga
    21781 ttgagatcaa accgtccggt gtagaccaga tccgccatct gcagcagttt gtctgactgc
    21841 cagttcgcca gcgaattgcg gtattcccat tggctaatga atgctggcag agattctggc
    21901 ttaacgcgga agaagttaac atcgacatta ttaacgttga gcgccattac cggcagccct
    21961 tcaacgactt tgccaggcag cagcgaacca cggctggcaa aaccgacgct gggttggatg
    22021 tcgcgggtag ttatagtttt ttcgtaatct ttactgaagg ttgcgttgtt gagtgctttg
    22081 acctccttgc caatagtaac gatcaaatca cgtttcggtt cgaggtggcg taaacgtagc
    22141 tctttaagat tatctgacag ctcccaggca ccatccactt tgccgctttt tttatcgacg
    22201 acatgaataa cgcgtgagaa atcctgatcc gggtcgagag ggatggagaa cgtcagcacc
    22261 agcgtggctg caccatcaag ttggacttca gagagatcca gcaatgtcag caccttaccg
    22321 gcactctgtt gggccagttt ttgtctctcc ggcacggaga gttttgctga actcgcgttt
    22381 tctgaagagg ccgctttagt aacttcagaa ggcgcatctt ttttcaccgc tgttggcgcg
    22441 ttatcgttgt tgtcgcaccc tgccagcgcc agcattagca tgcaggcggc tacgcgtaac
    22501 tttttcattt ttcatccctg ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg
    22561 tgagaattta cgttatgtaa ttcagtatca cgcccataca atccgaattt tagtgagaat
    22621 tgtggctcag acagccaaat cagcctcttt cgcttgtcgc ttttcactaa acatccgaca
    22681 atttagccta accccggcaa aaatggagat gcctatgtcc acgacatggt ttgtaggagc
    22741 cgactggctc gccgaacata ttgatgaccc ggaaattcag attatcgatg cccgcatggc
    22801 gtcgcctgga caggaagatc gtaacgttgc tcaggagtat ctgaatggac atattcccgg
    22861 cgcagtgttt tttgatatcg aagcgctttc tgatcacact tccccgcttc cgcacatgct
    22921 gccgcgcccg gaaacgttcg ccgtggcgat gcgtgaatta ggcgttaacc aggataagca
    22981 cctgattgtc tatgacgaag gtaatctttt ctcagcccca cgagcatggt ggatgctgcg
    23041 cacctttggt gtagagaaag tgtcgattct ggggggtgga cttgcaggct ggcagcgcga
    23101 tgatctgctg ttagaagaag gtgcagtaga gctgccggaa ggagagttta acgccgcgtt
    23161 taatcctgaa gccgtggtga aagtaaccga tgtattattg gcaagccatg aaaatacggc
    23221 gcaaattatt gatgcccgcc cggctgcacg ttttaacgca gaagttgatg aacctcgccc
    23281 aggtttacgt cgcggacata ttcccggagc actgaatgtt ccgtggacgg aactggtgcg
    23341 cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc gcggcgtcag
    23401 ctacgacaaa ccaattatcg tcagttgcgg ctctggtgta acggcagccg tggttttgtt
    23461 agcactcgcg acgctggatg tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg
    23521 gggcgcgcgg gcagatttac cggttgagcc agtgaaataa gtattttaca ggcaataaaa
    23581 aaccgccgaa tttggcggtt ttttattgct ggtctggttc gcggcctttc cagcaggttg
    23641 tattaccgta gtaatgcaag cgcgtctcag cggagacaat actcgccagt aactctcttt
    23701 ttgtcaagca aaagagagtt attattgttc tgttagtgta ttatccactg cggccctttc
    23761 cgccgtctcg caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag
    23821 cgcgtctcag cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt
    23881 cgcatggacc gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg
    23941 ctgttttccc aagtaaataa taaccggagt ctctccggcg acaatttact ggtggttaac
    24001 aaccttcaga gcagcaagta agcccgaatg ccgccctttg gggcggcata ttttagatta
    24061 tccgattctg tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcacaa
    24121 tatgttcggt aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga
    24181 ttggttcatc gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga ataatttctt
    24241 cgatatcacc atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt
    24301 taattgaaca aataaaagca cgcttcaccg acttaatggt tttaaataag gtggtgagtg
    24361 aatcaatcat ttgtgctggc ggctctgcga cttccgataa tatcagcgat tcaccgcctt
    24421 ccaggatttc ctggctgctc agcggatttc cctcttcacc aatcaacaaa ctgatttcac
    24481 gcggcataaa ttctttaccg gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg
    24541 tcatctcaaa caatgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct
    24601 gttgaagcgc ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc
    24661 agtgctgtaa atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag
    24721 gcacccagac ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg
    24781 ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt ttcggacata tgaaccacaa
    24841 ttcattcaac gttcggggcg caaatgcctg atgcgctacg cttatcaggc ctacaaggga
    24901 ttcgcaattt gttgaatttg cagaatttgt aggccggata aggcgtttac gccgcatccg
    24961 gcatattagt ttacgccgtt aacagattag ctatcgtgcg cacaccaagt cccgtagcgc
    25021 ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc
    25081 agccttgctg atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg
    25141 ccggatacgc cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc
    25201 ggtggaattc cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca
    25261 gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg
    25321 cagttttcgc cgccccggtg agggtcgccg catcaatgat catttccggt ttctgcgcac
    25381 tggcatcaat cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt
    25441 caactttttt accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa
    25501 tcaggttatc cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg
    25561 caaatgccag cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca
    25621 taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca
    25681 ggcacgcgta caccggtgct tctttatcgc cagttgggtt gtaatccagc gccagcaata
    25741 ccggagaacg ttctgaaccg cgcccgacgg tgtgcagccc catataacct tgctcacgca
    25801 gatcttcgcc tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat
    25861 caacagcacg ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt
    25921 cacgcaccca gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca
    25981 gatccggcca ctccacttta cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc
    26041 agcagcgatc cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt
    26101 caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg
    26161 taatgccgtc attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt
    26221 gggtagagag ggtaatcttc atcgcttctg tcattttagt tatccttctt tgtaaaaggg
    26281 ccgccagttg gcagccccag gatttttccg ctaaatgatt cgcgttgcag gaaggcggca
    26341 agtgagtgaa gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa
    26401 cgcatctgcg gcgtgaagca tgacgcggaa attattcggc ctcgtccagc cagactaaca
    26461 aaatcgcttc gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat
    26521 cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg
    26581 catcgtacag tgcttcgcca atttcgcggc tatcggtcca tttaagtccc atactaacct
    26641 ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacca ctaaatcttc
    26701 gtcggtgacg cgcgcctggc agcttaaacg gctttccggc tccagtcccc aggctttgtc
    26761 cagcatgtcg tcttcctgct ctgagctttc cggcagcgag tcaaaacctt cacgaacgat
    26821 gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt
    26881 acgcagagct gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg
    26941 gcagagatcc tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat
    27001 ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt
    27061 gtttgtctac gtttttaatc gcttgttcga tggcgtcaac gtcatcgccc tgcgccactt
    27121 cactcaggtg agcggcagca tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg
    27181 catcagcagc cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt
    27241 ctgccagcat tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg
    27301 aagcgatttc gctatcggtc agaccgtaag acggtttgac ctgaatagac gcctcaacgc
    27361 cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca
    27421 cgcgaatatg cgcaccacca gccggtagcg ccggaatacc acgcagcgca aaacgcgcca
    27481 gtgagcggca gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg
    27541 tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac
    27601 gcggaatcac tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca
    27661 catcaagcag cagcatttcg ctgtctggct tgttacccac cagaatatcc gcctgaatcg
    27721 ccgcgccaat agcgacgact ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa
    27781 attcgcctac ccgttcacgc accagcggca cgcgagtaga accgcccacc atcaccactt
    27841 ccagcacttc atcagcttct acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg
    27901 ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc
    27961 agcccgcaac gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg
    28021 cggcatccag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct
    28081 cgcgaatgta atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc
    28141 cggttgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc
    28201 cgccaccgag gtcataaaca gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga
    28261 tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg
    28321 cgtctttggt gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataactacac
    28381 catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg
    28441 cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccgtttt
    28501 cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc
    28561 gtcccatcag gcgtttaaca gaactaatgg tgttggcggt atcaagcgct gcgttagtac
    28621 gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt
    28681 gacggccttc atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg
    28741 agttggttgt gcccaggtca ataccagccg ccagacgacg ctgatgcggc gcagcactca
    28801 aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc
    28861 agtttttctt cgagttgttc ggcactgctc cgcagtttat cgagaaaacg cagcttacgc
    28921 acggtatccg ccgccgcgtc ccacgcctcg ttgtctaact gttcaaccat caactgatgg
    28981 cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc
    29041 gcctgttcga tctcgtccag ctcttcgcgc agctccaact gttccatcag gaacgcggtg
    29101 tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc
    29161 gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt
    29221 acggcggcga gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt
    29281 tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag
    29341 ccaaagaggg tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg
    29401 aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc
    29461 aaacgtggaa gctttcgccg caaccacact catctttgac gttcgggttg gtgaatttaa
    29521 acccttcgtt caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc
    29581 ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg
    29641 gttcgtcaac aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca
    29701 gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac
    29761 tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt
    29821 ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc
    29881 accggcggca gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg
    29941 agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc agaaccgcag
    30001 ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac
    30061 ttcatcacgt cgccacaggc cggtgccccc accatgccgc tgccgacgtt ctcgtcgttg
    30121 ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa
    30181 gccattataa attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc
    30241 agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg
    30301 gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa
    30361 gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag
    30421 gacggttcga ggcttgctga cgtacaggcg gaacctgaag aaactgcgag gtctttcagc
    30481 gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca
    30541 ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga
    30601 cggttacgca ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga
    30661 taggcctcgc ccattccgac gatctggtga acaggcagag tgccggaacg cataccgcgc
    30721 tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac
    30781 agcgcaccga tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact
    30841 ttcaactggc tcaggtcgat aggcagtttg cccacgctct gggttgcatc aacgtgatag
    30901 ataatgccac gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt
    30961 tcgttattta cgtgcatgat ggaaacaagg atggtgtcgt cacgcatcgc tgcttcaagt
    31021 tctttcaggt cgataatgcc gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg
    31081 cgctccagct gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg
    31141 tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg
    31201 gttgcaccag aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga
    31261 ttacgggcga tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgagaggcc
    31321 gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc aacacgcggg
    31381 tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact
    31441 ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg atgtacgacc gtgtttacgg
    31501 agcgcgatac gccccggcct gattctgaat tctttttatt aagcgcgtaa cttaacgtcg
    31561 atcgcgtctt gtatgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc
    31621 agcacttcct ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga
    31681 cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg
    31741 cctttaccct gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca
    31801 acggcgatgc tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg
    31861 gaaaccagac catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt
    31921 ccctgacgtt cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg
    31981 tcaagcattg cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc
    32041 tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt
    32101 caagtctgac attcccgagt aaattggtca actatttact tgactgattt agtcgggtat
    32161 ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc tgttgtcctt ccagaccatg
    32221 ggcgggttga gggcaattat tcgcattagc gctggcctct tcccctcacc ctaaccctct
    32281 ccccgaaggg gcgaggggac cgtccgtgca tttatctggc ttagccctca ggccttatta
    32341 cctttattct gctgctcaat agaagccaga atcccgcgca ggatattcaa ttcctggctt
    32401 tccgggcgcg cacgggtaaa cagacggcgc aatttattca tcacctgccc cggatggttt
    32461 tcacggataa aaccggttgc cagcagcgtt tgttccagat gaccataaaa acgctccaga
    32521 tcatcgacca gcggatacgg cgtctcttca tgctcgacct gctcgccgtt ttcctgagtt
    32581 gccagccagg ccatgcgcac ttcataggca ataacctgaa ccgccatcgc caggttcagc
    32641 gagctgtatt ccgggttagc cgcaatcgcg acatgataat ggcatttctg caactcttca
    32701 ttggtcaagc cgacgcgctc acgaccaaac accagcgcca ccggggtatt tgccgcttca
    32761 gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag cgtgcgggag
    32821 cgtgcgctgg tgcccaccac caggctacaa ccggctaacg cttcgtcgag cgtatcgacg
    32881 atgtgagcat taccaatcac atcgctggcc cctgctgcca gggcaatcgc ctgggagtcg
    32941 ggtttcacca gtggattaac cagccacaga ttggttaatc ccattgtttt catggcacgg
    33001 gcaacagaac ccatattgcc ggtgtgtgac gtctccacca gcacaattcg aatattttgc
    33061 agcattgtct ttcttcatct aaagattatt cacgcatctt atcataaaac gaagacagat
    33121 gccgatctcg ctgctatact ctgcgccgtt ttcccgttct ttaacatcca gtgagagaga
    33181 ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg taatttaatt
    33241 gccaaaaact atgaaacccc ggacgctgta gaagcgagcc agaaaggcag taacgatttc
    33301 gtgaccaacg tagataaagc tgccgaagcg gtgattatcg acacgattcg taaatcttac
    33361 ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg aaggtactga tcaggatgtt
    33421 caatgggtta tcgatccact ggatggtact accaacttta tcaaacgtct gccgcacttc
    33481 gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt atacgatcct
    33541 atgcgtaacg aactgttcac cgccactcgc ggtcagggcg cacagctgaa cggctaccgt
    33601 ctgcgcggta gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg cttcccgttc
    33661 aaagcaaaac agtacgccac tacctacatc aacatcgtcg gcaaactgtt caacgaatgt
    33721 gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc tgcgggtcgt
    33781 gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg cgagctgctg
    33841 gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg gtcataacta tatgctgacc
    33901 ggtaacatcg ttgctggtaa cccacgtgtt gtgaaagcca tgctggcgaa catgcgtgac
    33961 gagttaagcg acgctctgaa gcgttaatga ctcaggcggg tgatgtcact cacccgccct
    34021 cgcctttcag gcgctattct gaaatacttc ctcaccgctt tactttcttt cgtcactctc
    34081 ccaccatctt tccccgatta atggatgata aagaagtcgc aaggaaaggt tatggcactg
    34141 ccagtgaaca aacgcgttcc caaaattctg tttattctct ttgttgttgc cttctgcgtt
    34201 tatttagtgc cgcgcgttgc catcaacttc ttctattatc ctgacgataa aatttacggt
    34261 ccctacccct ggtcggcgga atccgtcgaa tttacggcta aggacggtac tcgtctgcaa
    34321 ggctggttta ttccttcttc gacgggccct gctgacaacg ccatcgcaac catcattcat
    34381 gctcacggca atgccggaaa tatgtccgcc cactggccgc tggtcagttg gttacctgag
    34441 cgtaatttca acgtttttat gtttgattat cgcgggtttg gtaaatcaaa aggcacgccg
    34501 tcccaagccg gattgctgga cgatacgcaa agtgccatca atgtggtgcg ccatcgcagt
    34561 gatgtaaacc cacaacggct ggtgctgttc gggcagagta ttggcggggc gaatattctg
    34621 gatgttattg gtcggggtga tcgtgaaggc atacgtgcgg tgatcctcga ctccacattt
    34681 gcctcttatg caaccatcgc caaccagatg atccccggca gtggctactt acttgatgag
    34741 agttacagcg gcgaaaatta tatcgccagc gtcagcccga tcccgctttt actcattcac
    34801 ggtaaagctg atcacgttat cccatggcag cacagcgaaa agttgtatag cctggcaaaa
    34861 gagccaaaac ggttgatcct aatcccggat ggcgaacaca ttgatgcttt ttccgatcgt
    34921 cacggcgatg tttatcgcga acagatggtg gactttatcc ttagcgcgtt gaatccacag
    34981 aactaaccca tgatcgctag cacgataatc attcacaaaa ccaccttaag acatgctaat
    35041 ccactggtca gaacagttta agatgagaaa aattctgtga cgcttgccaa catttctgat
    35101 gattagcatt cccttcgcca tttccctgag caaactttag ctattcttat caattatgct
    35161 tatgggagat ctacagatga tgcctacgct tgctccacca tctgtccttt cggctcccca
    35221 gcgccgctgc cagatcttgc tgacgctctt tcagccgggg ttgaccgcca ccacggcaac
    35281 tttcagcgag cttaatggtg tggatgatga tattgccagt cttgatatca gcgcaacagg
    35341 acaggagatc ctgcgctatc atcaactcac actgacggct ggttatgacg gtagctaccg
    35401 ggttgaaggt acagtgctta accaacgttt gtgtttattt cactggctac gacgtggttt
    35461 ccgtctgtgt ccatcattta ttaccagcca tttcaccccc gccctgaaga gtgaactgaa
    35521 gcggcgcgga attgcgcgta acttttacga cgataccaat ctacaagcgt tagtgaatct
    35581 ctgctcccga cgactgcaaa aacgctttga aacgcgcgat attcatttcc tgtgtctgta
    35641 tctgcaatat tgtttgctgc aacaccacgc tggaattacg cctcagttta atccgctcca
    35701 acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg caggaaattg gacgccactg
    35761 gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca ctgtttatgg cattactttt
    35821 ttccatgttg cgggttcccg atccattgcg tgatgcgcat cagcgagaca gacaattgcg
    35881 tcagtctatc aaacgtctgg taaaccattt tcgtgagctg ggaaatgtcc gtttttatga
    35941 tgaacagggg ttatgcgatc agctttatac ccacctcgcc caggcgttaa atcgcagttt
    36001 ttttgccatc ggtattgata ataccctgcc ggaagagttc gccagactgt acccacgcct
    36061 ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt gaatacggcg tccatctttc
    36121 tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc aggaaaacga
    36181 tctgcatgaa aaacagatta ttctactgac cgggaatgat agcgagcgag aagcgcagat
    36241 tgagcagcag ttacgcgaac taacgttact gccgctcaac attaagcata tgtcggtaaa
    36301 ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg attattgcgc cttataccat
    36361 gccattaccg ctcttttcac caccgctgat ctatacggac ctgacgctga caacacatca
    36421 acaggagcag atccgcaaaa tgctcgaatc agcatgaggg aacaactttc gggcgcaaaa
    36481 acattgccgg cagcgccacc agcgccatta cccagaacac gccgtggccc agatattgat
    36541 acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg acggcagagt
    36601 aaaccgcctg taaacggatg acttcgctac cctggcgagc agcaatataa cgcatggcgg
    36661 ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg caccactatc aaccacggca
    36721 acgccgtagt tgctcccata atgccccagc gcactacgcc gcaaatcgcc gagatcaaca
    36781 gcatatcgcg tgcactacaa cggcggaaaa gtttattact cagcgcaaag ataatgactt
    36841 ccgccaccac gcccagcgac cacaaatacc ccaccgccga ggccgagtag ccagctgcct
    36901 gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat aaacaaacgc
    36961 aggccagaaa gcgccagttc tggcgaacca gcgccaacca cgctgaccaa ccggtgctct
    37021 cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg gatgagaaag ccgagcagca
    37081 tggatgccac gcccaacgtc aacagcgcga ggatcacccg ataatcaaac atagtgacca
    37141 gtttgcccgt cagcgccgag ccaatgacaa acgccaccga gccccacagt cgcactttgc
    37201 cgtaatcaag cgggaactgc ttttgccacg tattcgccag tgcatcggtc aacggtacca
    37261 gcggtgagaa aaagaggtta aagccaatca tcaccagcat cagccacgct acgtgcgccc
    37321 ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc aaggcggaaa
    37381 tcaggcggga aggatcgctg acgcggggcg cgatgagcaa actcccgaga aaacgggcaa
    37441 ccagacctgc ccccaataac aggccgatgg tttctggcgt taaaccaatc cctttaagcc
    37501 agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa tagccgagcg
    37561 ccaaccagcg cgtggattgc aaaaccatga gtccctcccg tcaggaggcg ttagtctggg
    37621 gttaatcgaa tgttggtgca agttgaaaaa taagaatatt cgagcctgct tcccgatgtc
    37681 tgtaacgcca gttcacaagc actaaaaaac tgagatccgc gacgcatgtt gcaaaaataa
    37741 tgcgtaatta tgccgttacg cttgccaaac gttcctgcac aatggcgatg aaatcgcgta
    37801 atgccggctt catctcccct ttcttccacg ccatcagtaa agcaatggaa ggcacatttc
    37861 cggcaatggg acgaaaaaca acctgtccgg tattaaaatt attcatataa ccgggtatca
    37921 aagtgacgcc cagccccatg cccaccagat tcatggtcac cagaatattc gttgccacct
    37981 ggacgatatt tggctggctg ttttcttgcg caaaccacgc tttaacgatc ggcgcaagcg
    38041 aaccggaata cgccggatcg gtactgacga aattcacgcc atccagttgc gcggcggtga
    38101 tctctttttc atgtgctaaa gggtgatcaa ccggtaacac aaccactaat ggttcgtcaa
    38161 aaagctccag ataatcaatc tccgggctat aaacaggatg gcgcattaag ccgacgtcga
    38221 gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat taaactcacc agctcaatca
    38281 aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa ttcacttccg
    38341 ccgatggcac aaagccaatg gttaattgtc tgtcttcctg aacaattttc cgcgcccgta
    38401 atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa cattctcccg
    38461 ccgctgtcaa cgcgactttg cgcttatccc tcaccagtaa cggaacaccg acacagtttt
    38521 caagatcgcg gatctggctg cttaacgaag gctgtgaggt atgcagtttt tccgccgcac
    38581 gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg taaatgccgt agttccatca
    38641 ccttcccctt gttatcgaaa aaacgtctca gctggtagaa attaactatt tcacatatta
    38701 gcaaccaacc agcaacatcc ttatggcaca aaaatagaag gtcaatacat cttatctttc
    38761 aggattaaaa aatatgacca caccctcaga tttgaacatt taccaactga ttgacaccca
    38821 aaatggtcgg gtcactccgc gtatttatac cgacccggat atttaccaac tggagcttga
    38881 gcgtattttc ggtcgttgct ggctatttct cgcccacgaa agccagatcc caaaacccgg
    38941 tgatttcttt aacacctaca tgggagaaga tgcggttgtc gtagtgcgtc agaaagacgg
    39001 cagcatcaag gcgtttctca accaatgccg ccaccgggcc atgcgtgtga gttatgcaga
    39061 ttgcggcaac actcgcgcct ttacctgccc gtatcacggc tggtcttatg gcattaacgg
    39121 cgagttgatc gatgtaccgc tggaacctcg cgcctaccca caagggttgt gtaaatccca
    39181 ctggggacta aacgaagttc cttgtgtgga gagttataaa gggctgattt ttggcaactg
    39241 ggataccagc gcaccgggcc tgcgtgatta cctgggtgac attgcctggt atctggatgg
    39301 catgctggat cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa agtgggtgat
    39361 caactgtaac tggaaattcc cggcagagca gttcgccagt gaccagtatc atgctctgtt
    39421 cagccatgct tctgccgttc aggtattagg ggcgaaagat gatggcagcg ataagcgcct
    39481 cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc tgcaatttgg
    39541 tcaggacggt cacggtagcg gtttcttctt tactgaaaaa ccggatgcta atgtctgggt
    39601 cgatggcgca gtttcaagct attaccgcga aacctatgcc gaagcagaac aacgtttagg
    39661 tgaagttcgc gccctgcgcc tggcgggtca taacaatatt ttccccacgc tttcatggct
    39721 aaacggcact gccacactcc gcgtctggca tccgcgcggc cctgatcaag ttgaagtgtg
    39781 ggcgttctgt attactgaca aagccgcctc cgatgaagtt aaagccgctt ttgaaaacag
    39841 cgccactcgt gcttttggtc ctgctggttt tctcgagcag gatgactcgg agaactggtg
    39901 tgaaatccag aaattgctta aaggccaccg cgcccgcaac agcaaactgt gtctggaaat
    39961 ggggcttggt caggaaaagc gccgcgacga cggcattcct ggcattacta actatatctt
    40021 ttcagaaact gccgctcgtg gaatgtacca acgctgggcc gatcttctga gtagcgaaag
    40081 ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag gaggtgatga aatgagtgcg
    40141 caagtttcac tagagttaca tcaccgcatt agccagtttc tctttcacga agccagctta
    40201 ctggacgact ggaaatttcg tgactggctg gcgcagctcg acgaagagat tcgttacacc
    40261 atgcgcacca cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca gccaccgaca
    40321 acctggattt ttaatgacac caaagaccag ctggagcggc gaatcgcccg tctggaaacg
    40381 ggcatggcct gggcagaaga gccgccgtca cgcacccgtc acttaatcag caactgccag
    40441 ataagcgaaa ccgacatccc aaacgtattt gctgtgcggg taaattatct gctttatcgg
    40501 gcacaaaaag agcgcgatga aacattctat gttggaacgc gtttcgacaa agttcgccgt
    40561 ctggaagatg acaactggcg cttgctggaa cgggatatcg tcctggatca agcggtaatc
    40621 acttcccata acctgagtgt actgttctga tgaatcgaat ttatgcgtgt cccgttgcgg
    40681 atgtgccgga gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc ctgttcaacg
    40741 ttggcggcga gttttatgcc attaacgatc gttgcagcca tggtaatgcg tcgatgtcag
    40801 aagggtatct ggaagatgac gccacggtgg agtgcccgct acacgccgcc agtttttgcc
    40861 tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact tatccagtac
    40921 acgttgaagg tggtgacatt ttcatcgact taccggaggc gcagccatga gcgatctgca
    40981 taacgagtcc atttttatta ccggcggcgg atcgggatta gggctggcgc tggtcgagcg
    41041 atttatcgaa gaaggcgcgc aggttgccac gctggaactg tcggcggcaa aagtcgccag
    41101 tctgcgtcag cgatttggcg aacatattct ggcggtggaa ggtaacgtga cctgttatgc
    41161 cgattatcaa cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg attgttttat
    41221 cggcaatgca ggcatctggg atcacaatgc ctcactggtt aatactcccg cagagacgct
    41281 cgaaaccggc ttccacgagc tgtttaacgt caacgtactc ggttacctgc tgggcgcaaa
    41341 agcctgcgct ccggcgttaa tcgccagtga aggcagcatg attttcacac tgtcaaatgc
    41401 cgcctggtat cctggcggcg gtggcccgct gtacaccgcc agtaaacatg ccgcaaccgg
    41461 acttattcgc caactggctt atgaactggc accgaaagtg cgggtgaatg gcgtcggccc
    41521 gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg aaacctcgat
    41581 aatgcagtct ctgacgccgg agaaaattgc cgccatttta ccgctgcaat ttttcccgca
    41641 accggcggat tttacgggtc cgtatgtgat gttggcatcg cggcgcaata atcgcgcatt
    41701 aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt cgcggcattc gccacgtagc
    41761 ggctgggctg gatctttaag gaagcacgat gaaagaaaaa accatcatta ttgtcggtgg
    41821 cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag caagggttca ccggtgagct
    41881 gcatctgttt tccgatgagc gacatcttcc ttatgaacga cctccactct cgaaatccat
    41941 gttgctggaa gattccccgc agttacagca ggtgttaccc gctaactggt ggcaggaaaa
    42001 caatgttcat ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca cacgagagtt
    42061 agtgttaacc aacggcgaaa gctggcactg ggatcagctt tttatagcaa ccggcgcggc
    42121 agctcgaccg ctgccgttgc ttgatgcact gggagaacgc tgctttaccc tacgccatgc
    42181 cggtgatgcc gccagactgc gagaagttct gcagcccgaa cggtcagtcg tgattgtcgg
    42241 tgccggaact attggtctgg aactggctgc cagcgccacg cagcgcagat gtaaggtgac
    42301 agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg tgcaacgcta
    42361 tcttttacag cgccaccagc aggctggtgt gcgcattctg ctcaataatg ccattgaaca
    42421 tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc ttcaggctga
    42481 tgtggtgatt tacggtattg gtatcagcgc caacgagcaa ctggctcgcg aggccaacct
    42541 tgatactgcc aatggcattg tcattgatga ggcttgccgc acctgcgatc ccgcgatctt
    42601 tgccggtggc gatgtggcaa tcactcgtct tgataatggt gcactacacc gctgcgaaag
    42661 ctgggaaaac gccaataacc aggcgcaaat tgccgctgcc gcaatgttag ggctaccgct
    42721 accgctactg ccgccgccgt ggttctggag cgatcagtac agtgataact tacagtttat
    42781 tggcgatatg cgtggcgatg actggctttg tcgtggcaac ccggaaactc agaaggcgat
    42841 ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc aggggcgtga
    42901 gattcgccca attcgcaaat ggatccagag cggcaaaacg tttgatgcga aactgctgat
    42961 agatgagaac atcgcgctta aatcactgta accaggataa ttagcgaata tctcaatgcc
    43021 tggggcgtgg cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc ttgcaaaggg
    43081 attggttatg aacacactac gttattttga ttttggagct gcccgccccg ttttgttatt
    43141 aattgcccgt atcgccgtgg tcttaatttt cattattttt ggttttccca aaatgatggg
    43201 ctttgacggt acggtccaat atatggcctc gttgggcgcg ccgatgccga tgctggcagc
    43261 gattattgcg gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg gctttttcac
    43321 ccgtccgctg gcggtgctgt ttattttcta cacgctgggt acggcggtga ttggtcacca
    43381 ttactgggat atgaccggcg atgcggttgg gccaaatatg attaatttct ggaagaatgt
    43441 cagtatcgct ggcgcattcc tgctgttggc aattaccgga ccgggggcga tttctctcga
    43501 tcggcgttag gaaagatgcc ggatgcggca tgaacgcctt atccggcatt ttataaatta
    43561 cagcaacgca acccgcaacg acatctctga agttgttgat tcacctggtg caagcgcaag
    43621 aagatcaccc ccttccgggc gatgatgatc atccggcgca tggctcatcg gttccagaca
    43681 gaagaaatca aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga tgaaataaca
    43741 cggtgcaggt ggcgtcgttt ccatgatgat ggcataacct tcctgcggct gctcgatgcg
    43801 ggcctgacca ttccatccgg caaaaccatt gttcacccac tggcgcggca acggcgcggg
    43861 ctggttaaaa tccagttcct gcggtagctg ctcgcaaaac tcacccgcca gccactgctc
    43921 ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg acaacggaaa
    43981 ataaggatgc cagccggtac caaatggcag cgtctctgcc ccctgattgg tgacagagag
    44041 cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga cttacccgat agtgatagac
    44101 accgctgcga tgttcataca ccagacacaa actatcatcg ctgtgcgaga cacattgcca
    44161 ctcgcccagc cagccatcgc cgtgtagata gtgcgcatcc cactcaacat tcggttgcag
    44221 ttgatactca cgcccctgcc agacaaaacg attgccgctc acccggtttg caaatggcac
    44281 cagcggaaaa catgaggcat cggttgccac accgcttttt ttaccaggac gtaagagcgg
    44341 cgtcgtatcg cgccagaagc cttcgattac gccgccctgg tcagaaacgt ccagctttag
    44401 cgacccgtgg gataaggtat agatggtcat ccgctaactc cttaatccgg gaagttaatc
    44461 acaactttcc cgcatttgcc gctcgccatc agcgcatagg catcacctgc ctgttccagc
    44521 gagaagcgat gggtaatggc gttacgcggc cacagcttcc agtccgtcag atcatgggcg
    44581 catttttcca tatggaacag actggtcacc caggagccga taatccgccg ttgatggtgc
    44641 atcagatcgg cgctgacctc gaattccact tttccggttt caccaatgta aaccacccgt
    44701 ccccagtcag cggtggattg cagtgccagc aagcgacctg cggcattacc ggaacaatcg
    44761 agcgcaacat ccgcgccacc gtgggtgagt tcggcgataa tctgcggcag accttcggtg
    44821 gttgctaaat agccgtgatc catcacccct aactgttttg ccatcgccag acgttccggc
    44881 agcatatcaa cgccgatgat ccgttttgca ccgcgacctt tcgccagcat catcgccatc
    44941 atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac ttcgccgcgc
    45001 aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc ttcgtagctc
    45061 agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg
    45121 ccgtcacgct gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg
    45181 cggcggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc
    45241 tctttaaaat ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg
    45301 ttgataaatc cttggtataa cggtttgtcg ggtgccgctg ccgtagcgcg gtgctggtgg
    45361 tagatatagt ggacatcgct tccgcaaatt ccggaagatt tcattttgat cagtacctgg
    45421 ttaatccctg gtgtcggcac cgcaacttcc cgtagatcga cggtcgaatt tcctggtaaa
    45481 taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct
    45541 gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca
    45601 gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa
    45661 aggagttaat accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa
    45721 gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg
    45781 cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccagaa ccgaggcgcg
    45841 ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc
    45901 gcacccgacg aacgttgatg ccgcacaact gcgccgccgt agcattaccg ccaacggcaa
    45961 aaaccgagcg cccgaaggcg gttttgcggc tgatgaacac aaacagcgca aacaacacaa
    46021 tcatgatcag cgcggatacc ggcacaccga gaaattgtcc gcccagccag tccagcacct
    46081 cgttttcgtc aatcggcact ggcagtgcgt tcgtcataaa cagccccatt ccgcgcaggg
    46141 cgctccacag ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc
    46201 cggccagcgt tcccatcagc gcgcctaaca gcaacaccag cagacacgcc accgccagcg
    46261 gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa
    46321 cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa
    46381 tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaag ccaggcgcgt
    46441 tcagggagaa gaccagataa agaatggcga ttaccaccag aaaaccgatc tcattaatat
    46501 ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatgat aatgacgaag
    46561 cagacatctt caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca
    46621 gctcatccac attgaccggc gagtgaaact cctgcgagaa cgtaccgtgc tgtaacaaca
    46681 ggatgcggtc acacaccagc ggcaactcct ccacttcact ggagataaac accacgcttt
    46741 ttccttcggc agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga
    46801 cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga
    46861 tcaccacttt ttgctgattg ccgccagaaa gcgtgccgat gggcgtttcg ctactggcgg
    46921 ccttgaccgt catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca
    46981 gtacgccgtt ggcgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca
    47041 accagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca
    47101 gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt tcgccctgtt
    47161 catactcctc cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc
    47221 cagcaatgcc aagcacttcg ccgcgacgta acgtaaagct gatatcctcc agcttgggct
    47281 tatggcgtaa tgcacggact tccagcacgg cctgatccac aatttcctga ggggctaccg
    47341 gcgcaatatc aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt
    47401 tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa
    47461 tgcggcggat ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca
    47521 tctttttcac cgcactgatc accagttcaa cttctgcact ggcaagcgag ctggtgggtt
    47581 catcaagaat gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc accagttgct
    47641 tttgcgccgg gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg cccagcgcct
    47701 gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcgag
    47761 gccattgacc aaggcagagg ttttctgcca ccgtcagccc ttccaccaga cttaattcct
    47821 gataaaccgc acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt
    47881 ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagca
    47941 ttcgaatgag agtcgatttg cccgcgccgt ttttacctaa cagcgcacga acttcgcctt
    48001 tattgagcgt gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg
    48061 ccaccacttt tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc
    48121 agcgattagg gcagaccatc aacgtgcgtt gccagccact gtttaccgtc ttccgttttg
    48181 gtatagagat cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc
    48241 ttcaatgttt gcgcgaaaac agcattgccc attttcttac cggaaatatc caccaccgct
    48301 ttcaacacct gattgttttc cagctcctga gcaatttcag tggtcatatc cgaaccgaaa
    48361 acagcaattt ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca
    48421 ccgcccgatt cccccataat ggcgttgaga tccggcgtgg agataattag tttttcacca
    48481 acggaaatcg ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga
    48541 acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct
    48601 tcgcaattga tgacggcaat tttcggctgg tcaattttat tggcaataaa ataatcggcg
    48661 gcagcgttac ccagtttttt accaaattcc agcggatcgc cgaccagata cgccgagaca
    48721 tatttatcga cccccttttg attaatacag gtgttgtagc aaatcaccgg aatacccgct
    48781 tcactggcgc gacgaacggt acggctactg ccattttcag acactgccga taaaataatg
    48841 gcatcgacat tacgcgccac gagggtatca acaaaggtac tttctttcga aatatcaccc
    48901 tgggcgttag tttcaattaa ctgtacctgt actgaagaat cttttgccgc atcctgaacg
    48961 ccctgacgca ctccagcgta atatccctgg gtatcaaggt atattgcacc aatggtcatt
    49021 tctttatccg cagccctggc aaatagtgcg ctacctaata gcgtagccat taataataga
    49081 ttacgggttg ttctcatttt tttaggcata gtgcctcctg tagggttttt attaacaacg
    49141 gcttattcta attattttgt gatgagcggc agcgcgtgcc gctcaaatat tacagttaat
    49201 agggtttagt gaacgtagtt aaaaataaaa ggcatcacgg tggtgaataa tgccgctttc
    49261 ggccaggccg gattaatcgc cgtcaattca gcgcgagcag attcatattg cgcaacctcc
    49321 ccgagtcccg cggacgccag catcgccacc atcaggcatt tttctttatg ctgctgttgt
    49381 aaatcgccat acagcgacaa caggtcaggt tgtgaaacag caaagaaatc cgcctcaata
    49441 ctggttttcg ccatctcttg cgcccactgt ttcatttcac taaacagttg ctgtgcggtt
    49501 tgctgttcac ccagcagtcg cagcgccatt ccttgccaga agagataatc aaccggctga
    49561 tcgttgtaat aactgtggat gttaatggtg cgatcgccgg tcgccgccag acgtaaacaa
    49621 cgcatcgctt cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca gaaccagatg
    49681 tcgttatcag tttgccccgg taaacggcct tcgcttaaat tctccggata atgcagcgcg
    49741 gcatgaagca gttcgctggc ctgctgcggc tgtctggcat caagatgctg ccaggcgcgt
    49801 aataactggt tgaggataaa ctgactggtg accttccctt ccccgccttc ccacgggtgg
    49861 aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct gacccgtgag atgccacaaa
    49921 ttgagcagtt ctgcggtcat gtcgtcgcgt ttcagcgcaa tttccagatt attttccaga
    49981 cgcgccagtc gtttctccgg tgtggctcca cttaacttat ccagcaaatc ccgttcgaaa
    50041 agcagacgtg catcctgcgg cgcaagctga taagcattat caagataacg cgcggccagt
    50101 tcataatcgt gttgcttatt ccacgcatgg atcgccaacc cgcgccagcc atcggcaaac
    50161 tccggcgaca tctctacgca actttgccat aaagtaatgg ctttgttgta gctgcgcttg
    50221 ttgtagtaga agcaggccag taaatgacgg gcaaaccagc actcttcaat actctccagc
    50281 gccgccactt cttccagcgt attcgggaaa cggacaaact gcgggaagac atcaatggct
    50341 tttgcgacca gttcgccgcg ttcggctttc ggcagcaagc tggcttgtag ataaagcgat
    50401 aacgtgcgct gacagtccag tgcgttcagc atctctgtcg ccagagtggg cattccccag
    50461 ttaatcagtt gcccggcggt catcagggcg ttaacgtcgc gtccctgaca cagaccgcgc
    50521 cactgcgcga gagctgattc gctacgacca tcgaaccagt tcagccacca cagagtggcg
    50581 ttcagcggat aatcgcgcag cagtttctcg cgctgcacac gcgcgttgtc ctgacgacca
    50641 cttaacacca gcagcagatt atgcaggcaa agcacttcct gattggttgg gcaggcacga
    50701 agactttgtt ggcaaaaatc cagaccagcg tcgaagttac cattacgcgc cgccagtcgt
    50761 gccaggccat aatagccacc ggctttgctg ttgccgctcc agaccgcacg ccagaaatcc
    50821 tcttcggctt gttgatattg tccctgacgt tcgtaagcac tggcgcgaat caaactcgcc
    50881 tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt tcagagcctg agtggcatac
    50941 gccaccgctt gcgggaaatc ggcacggtta tattccagca tcgccagcgc caggttacag
    51001 cggtaatcca gcggatccag cgccacgccg cgcaggtagt aatcgaacgg tgaacggctg
    51061 gcgtgatgat attgctccag atgctgaccg ataaaccagg cttcatctgt actggtaatg
    51121 tcttgtgctg acagtggcgc tttggcgacg tccggcaacg gcaactcttg cggctgatgt
    51181 tcctgataac tcaggacaat attgccgtcg gcatcggaga gctcaatggt cagcctttca
    51241 ggattgatac cgtgcaacac gccctggatg gcggtcgcag gcatcagtgc cacggcatca
    51301 tcaagtaacg cgttgcattt gccgatttcg cggatcgcca ggcggtatcc gttcaacgga
    51361 gagatggcat acagccccca ctcaatcccc cgctcactac gctggagttt tatcaccgca
    51421 tcgcgggagg cattttgcac catgcccaaa gaatgataag gcaggaaata ctgctcaaaa
    51481 cgcttctctt cgtaagcatc aagccaggta aaatcaggct ggttatcggc aaaaataccg
    51541 gtcatcagtt cgatatacgg gccgttattg tcggtcagac tcttgtccca cgcctggccg
    51601 aattcactgt gtccccaact ccactgtttt ttacctggcg caatatggtg gttggcaacg
    51661 tgcagcaaac cgccatcttc atcgtgacac cacgcgccga caaaatcgta ctgcgatttt
    51721 tcggccatat atgaggttgg aacaggcaca tttttatagc gagaaatgtc cactccggcg
    51781 gagtagtcca ctttgtagta agtgccggtg gcgatgggga aagcggagac ggcccgtttg
    51841 ccgtgatcaa acaccgcagt cacatccggt gggaaaacgc tctgatgccc ttccccccct
    51901 ttcactgccg ggttggccca ccacaagaaa tgacgcggcg tggcgttacc gttatagacg
    51961 cggctggcga tttccagcgc cgcccggtca gggcgcaggg tgaaacctgt catcacctgt
    52021 aaaccatgca tcggctccgt ttcgccgacc cacaccgtct gtgcaccgtc ttcatgggct
    52081 tcgagggtga aatcaacggg cataaaggtg gtcgggcgat ggtgttgcgg ccagttaaac
    52141 tcaatcccac cggagatcca cggtcccagc agccccacca gcgcaggttt aatgacttca
    52201 ttgtgataaa caaaatcgcg ctgtttcact ttatcccacg cgcgatgcac ccgaccgccc
    52261 agttccggca ggatcatcac tttgatgtag tcgttttcca gccacaccgc ctgccaggat
    52321 ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt agggataaac cgcgccggac
    52381 gatccctgat aaacgcgatt ttccaggaac atgggatgta tatcctgcgg cccggtttca
    52441 taggtcggga tctcaacgcg ctcttgccac acttttactg gagtcatggt gccctcaata
    52501 ttaacaagac atactgaatt aaaagatttg tggcagtgta ttgaacaatc tggcaatgtt
    52561 ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt gaagatgaga gcctgcatta
    52621 acaatcaaca gattcgccac cataacaaat gcgtgattct ggaactgctg taccggcaaa
    52681 agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat ttcgattccg gcagtcagta
    52741 atattttgca ggaactggaa agcgaaaaac gggtggtgaa tatcgacgat gaaagccaga
    52801 cgcgcgggca tagtagcggt acatggctga ttgcgccgga aggtgactgg acgctgtgcc
    52861 tgaacgtgac gcccaccagt attgagtgtc aggttgctaa tgcttgttta agtccgaaag
    52921 gcgagtttga gtatttacag attgatgcac cgacgccgca ggcgctgctg tccgaaatcg
    52981 aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg caccatcaat ctggcgctgg
    53041 caatccacgg tcaggttgat ccggtgaccg gcgtgtcgca aaccatgccg caagcgccgt
    53101 ggacaacgcc ggtggaggtg aaatatctgc tggaagagaa gctcggcatt cgggtgatgg
    53161 tcgataatga ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat tcgcaggaac
    53221 gggatttctg cgtgatcaac gttgattacg gcattggctc gtcgttcgtg attaacgagc
    53281 aaatttatcg cggcagtttg tatggtagcg gacagattgg tcacaccatc gttaatccgg
    53341 atggcgtcgt ctgcgactgt ggacgttacg gctgcctgga aaccgtcgcc tcgttaagcg
    53401 cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc ggttaatact cagcttgatc
    53461 ctgaaaaatt gactaccgcg cagttaatcg ctgcctggca gagtggagaa ccgtggatca
    53521 ccagctgggt tgaccgcagt gccaatgcca ttggtttgag tctgtataac ttcctcaaca
    53581 tcctcaatat taatcagatt tggttgtacg gtcgcagttg tgcctttggt gagaactggc
    53641 ttaatactat tattcgccag acgggattta acccgttcga ccgcgacgaa ggaccgagcg
    53701 tgaaagcgac gcaaattggc ttcgggcaat taagccgcgc acaacaggtg ctgggaattg
    53761 gctatttgta tgttgaggcg cagttacgac agatttgatg gcgcgataac gtagaaaggc
    53821 ttcccgaagg aagccttttt tcaacagatt aaccgtttcg cttatgcgta aaccgggtaa
    53881 cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taacggcttc atcattgatg
    53941 ctgtccagca cgtcacacat ccagccagcc agttctttcg cttctgcttc tttgaagccg
    54001 cgacgcgtaa ttgccggagt acccacgcga ataccggagg tcacaaacgg gctcttcgga
    54061 tcgttcggta cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct
    54121 tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg
    54181 ccggaaacca ctttgtagcc gcgttccagg aacacttcta ccatcgcttt agcgttttta
    54241 gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct
    54301 ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc
    54361 agttttttgt acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc
    54421 gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag
    54481 acgccagcag caaccaggcc cgcaacgtgc gccatatcaa cgaacaggta agcaccgatg
    54541 ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc cggaatatgc agagaaacca
    54601 ccgataatca ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtagtcgata
    54661 tgaccggtag catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc
    54721 ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct
    54781 ggttccagca gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg
    54841 ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata
    54901 tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg
    54961 gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc
    55021 agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca
    55081 tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa
    55141 taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc
    55201 attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt caggctacgc
    55261 aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg
    55321 caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag
    55381 ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa aaggaagacc
    55441 atatgcttga cgctcaaacc atcgctacag taaaagccac catcccttta ctggtggaaa
    55501 cggggccaaa gttaaccgcc catttctacg accgtatgtt tactcataac ccagaactca
    55561 aagaaatttt taacatgagt aaccagcgta atggcgatca acgtgaagca ctgtttaacg
    55621 ctattgccgc ctacgccagt aatattgaaa acctgcctgc gctgctgcca gcggtagaaa
    55681 aaatcgcgca gaagcacacc agcttccaga tcaaaccgga acagtacaac atcgtcggtg
    55741 aacacctgtt ggcaacgctg gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt
    55801 ggggtaaagc ctatggtgta ctggctaatg tatttatcaa tcgcgaggcg gaaatctata
    55861 acgaaaacgc cagcaaagcc ggtggttggg aaggtactcg cgatttccgc attgtggcta
    55921 aaacaccgcg cagcgcgctt atcaccagct tcgaactgga gccggtcgac ggtggcgcag
    55981 tggcagaata ccgtccgggg caatatctcg gcgtctggct gaagccggaa ggtttcccgc
    56041 atcaggaaat tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc tatcgtattg
    56101 cggtgaaacg cgaagagggt gggcaggtat ccaactggtt gcacaatcac gccaatgttg
    56161 gcgatgtcgt gaaactggtc gctccggcag gtgatttctt tatggctgtc gcagatgaca
    56221 caccagtgac gttaatctct gccggtgttg gtcaaacgcc aatgctggca atgctcgaca
    56281 cgctggcaaa agcaggccac acagcacaag tgaactggtt ccatgcggca gaaaatggcg
    56341 atgttcacgc ctttgccgat gaagttaagg aactggggca gtcactgccg cgctttaccg
    56401 cgcacacctg gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg
    56461 aaggtctgat ggatttgagc aaactggaag gtgcgttcag cgatccgaca atgcagttct
    56521 atctctgcgg cccggttggc ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg
    56581 tgaagcagga aaacattcat tacgaatgct ttggcccgca taaggtgctg taatttgatg
    56641 ttgccggatg gaaacatccg gcaacccttg acgcggttta aattgccgcg tcgtcctctt
    56701 caccggtacg gatgcgaatg acccgtgcca cgtcaaagac gaagatttta ccgtcaccga
    56761 ttttgccggt ttgcgccgtg cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg
    56821 gtacgacaat ctcaattttc actttcggca gaaaatccac catatactcc gcgccgcggt
    56881 acagctcggt atggcctttc tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg
    56941 taataccgac ttcggccagt gcttcgcgga catcgtccag cttgaagggt tttataatcg
    57001 catcaatctt tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt
    57061 aacacataac gccaattcat tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca
    57121 gtttataaaa ttctgtccgg ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt
    57181 tggtaatttg cagcagctta cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa
    57241 aggttggcag cgccgtgttt tcaccctcca gcgcctgctc tactaatgca tcgctaataa
    57301 ccggagatga ggtcagcgcc acgcactgtt caatcacgtt gaccaactgg cgcacattac
    57361 ccggccaact agcggtcatc aggcgtttca tcgcatcagt ggagaacgcg cggacaaacg
    57421 gtttatgtcg ctctgccgcc tggcgcaaca ggtgatttgc cagaagcgga atgtcttctg
    57481 tgcgctctgc cagagccgga attttcaggc tgacaacgtt gaggcggtaa tagagatctt
    57541 cgcggaattc cccgcgcgcc atcgcttttg gcagatcacg gtgagtggca gaaataatcc
    57601 gcacgtcgat atcaatatcg cggttgctgc ccagcgggcg cactttacgc tcctgcaaca
    57661 cgcgcagcag tttgacctgt aacggcgcag gcatatcgcc aatttcatcg agaaatagcg
    57721 ttccgccttc cgccgcctgg aataaacctt cgcgattgct gacagcgcca gtaaacgcgc
    57781 cacgcgcatg accaaacagc tccgactcca gcaattgctc gggcaatgcg ccacagttaa
    57841 tagcaataaa tggtttgcta ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct
    57901 ctttcccggt gccgctctgg ccgttaatca aaacgctaac gtctgattgc gccaccagcc
    57961 gcgcctgttc cagcaaacgc agcatcagcg ggctgcgggt gacaatcgcc tcgcgccagc
    58021 gttcatcggt ggctggcgcg gattgttcca gcgcatcgtc aattgcctga tatagcgcat
    58081 ctttatcgac aggcttggtg aggaaactga acacgccctg ctgtgttgca gcaacggcat
    58141 cgggaataga accatgcgcg gtaagaataa ttactggcat tcccggctgc actttctgaa
    58201 tttcagcaaa cagctgcata ccgtccattt catccatccg caggtcgctg atgactaaat
    58261 ctactttttc gcgattcagt acccgtaatc cttcagcgcc actttccgcc gtgaccacgc
    58321 tgtagccttc gctggtcagg cgcaggccaa gcagtttcag caatcccgga tcgtcatcga
    58381 ccaataataa atgcgcaggt ttatggctca tcaggagtga cctcatgggt ggatggcgcg
    58441 ggcttttcac cctcgtgcgg cgtatccggc gagaaatttc cagccggttt gcgggtcgag
    58501 agctggcgtt caatatcagt caggttttcc agcttgcggg tggtgagttc cagttgctgc
    58561 tgtagaacgt gatgttgctg gcgcaatgta tccagctcgc tgtcgctggc ctgctggagc
    58621 ttgccgtagc gttggcgctc ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag
    58681 agttgataca gagggcgaac ctgtgccggg atctcggtac ttagcgcatc aatgcgcgcg
    58741 accagctggc ggcgctcata cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta
    58801 aaggtatttt gccagctgcc gtcgtcatat tggcgagctt gctgacgtga ctgcgcaggc
    58861 attaaacgat cagcacaatc catcgcccgc agccagtaaa gcggattggt ttcggttgat
    58921 ttaccttgca gcgcccagat gtcgctacat tcagtagaaa gatagtcagc cagttgataa
    58981 accggaattt tttcttctgc tggcgtatca atggctggct tattgtgact ctgcacgcaa
    59041 cccagcaatg ccagacatgg cagccctgcc agccacagcc gtcggggcaa taatcgttga
    59101 aaaatgtgtc gcatattcac cagacttaat gcctatccca gtgggcgtaa ttattgcagg
    59161 cagtttggac atggacagcg cggagaaacc ggagcgtaca cgtcgtacgt gaggatttcg
    59221 agcactgccc ggggccgaac tggcaaataa aatagcctga tgggataggc tgttagcatt
    59281 atttcgtgtt tttcgacgac ggcaattcaa tgcggaaaca aacgtcttgc ccgctctcgt
    59341 cgaccagata cagttcccct tgcatacggc gaatacaatc cctggcaatg cttaatccca
    59401 gaccgctgcc cttcaccgcc ccttttcgct ggtggcttcc ttgaaaaaag ggttcgaaga
    59461 tcatggcgcg ttcctcttgc ggaatgggtg tgcctgtatt gatgacatca atataaaccc
    59521 gtgcaccatg taagctgctg cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg
    59581 cattggagta aagattatcc agtacgctca tcagcagcat tggctccgcc aggcaagctg
    59641 ttgctttgag atcgacgtcg gtatgcatca ttttagcccg tgcgggcagg ctatgagcag
    59701 aaactactgt ctccaccagc ggtgctaact caacattctc cagttccacc gcactgtccg
    59761 cttgtttacg gttgtaatca agcagttgtt cgatcagttt ttgcaaattg cggctgctgc
    59821 tatcaagaat gctcaccacc tctttttgct ctggcgtaag cggcccgaca acctggtcag
    59881 ccagtaattc agtgccctcg cgcatactcg ccagtggcgt ttttaattca tgagataaat
    59941 gtcttaaaaa ttgatggcgt tgggattcca gccatgacag gcgctcactt aaccaaagaa
    60001 tacgttgccc aaccgagcgt aactcgctcg gtccactgaa cgaaacgcta ttgcccagag
    60061 aacgcccttc ccccagacgg ttgatcatgc gctcgatatt tttcaccggc ccgataatca
    60121 tccgcgtgaa aagtagcacc atcaccagac tcaccagaaa tagcaccagc gattgccaac
    60181 caaaatattg cccacgttcg gcgatttcac gctgaagttg ctgcccacga gagaacacca
    60241 ctgtgcgcgt ggcctgcacc atttcggtat tggcactggc aaaggcttca agacgcgcgg
    60301 cggcggcagc atcgggacca ctgttgttac actgaagttg ggccagattg ttcaaatcct
    60361 gacgcaatgc ctggtaaagt ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt
    60421 cgctgtaacg cttgcgctgg ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc
    60481 aatactgacg gtagctacgc tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc
    60541 gggcatcaat aagcgtagtg cggttaacca gcgccgcctg atcgctaagc gcattcaggc
    60601 tttgccaggc ttgccatgcc agcaccaaca ggggtagtag aattagcaaa aatgccagca
    60661 ttaccagttg tcgtaatgag cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt
    60721 cagacgcgaa tagcctgatg ctaaccgagg ggaagttcag atacaacaaa gccgggaatt
    60781 acccggcttt gttatggaat aaggcggtgc ctaactcgac gtttcgcccg atggttgata
    60841 tagctacgct gatatcagaa gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt
    60901 atgtagcacg tcccgaaggg gctgacataa gtcggtgaat gagccactgc ttactattat
    60961 gcagtaactg tgccaataaa gaaaatagtt tggtaacgta ctgattatac gttgctttga
    61021 gggatttatg gcttcttcgc tatttgaatg taaatcagcc actgtgtcgc caaaaagaga
    61081 caacttaaga taaacttatt agattacaat aaaatcaatc atttaagtgt cgccagaaag
    61141 cgacacggta aaccacccat tgtcgtgatt tacagacaca aaaaagctcc cggagttggg
    61201 agcttatgat agtggttggt gcttaatgcc ggatgcggcg taaatgccgc atccgggctg
    61261 caaaaccaat gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat
    61321 ccatgggcca tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac
    61381 acgttccggg tgcggcatca taatggtgac tcgaccactt tcagtcgtaa tcgacgtaat
    61441 accgttcggt gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc
    61501 gacatagcgc agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg
    61561 cacttccacg cgcccttcac catgagagac agcaatcggc atttgcgagc ccaccatccc
    61621 ctgcaacagc agcgacgggc tttgggtcac ttcaaccagg ctgaaacgcg cttcaaagcg
    61681 atcggaggta ttgcgcacaa aacgtggcca caactcacta cctgggatca gttcacgcag
    61741 attagacatc atctggcaac cgttacatac ccccagcgcc agcgtttgcg gacggtggaa
    61801 gaaggttgca aactcatcgc gtacacggtc attgaacagg attgacttcg cccaaccttc
    61861 accggcaccc agcacatcac cgtaggagaa accaccgcac gcgaccaggg cgtggaaatc
    61921 ttccaggccc gtgcgtccgg tcagcaggtc actcatatgc acgtcgatag catcaaagcc
    61981 tgcacggtgg aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagcac
    62041 agcaactttc ggacgtgcgc cagtggcaat atacggtgct gccacatctt cgttgatatc
    62101 gaacgacagt tttacattca ggccaggatc ggcatcgtta gatttcgcct gatgctcctg
    62161 atcggcacac tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca
    62221 gacacgcaac gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa
    62281 acggtcaccg gaaaccgcct gcccaacata atggacacaa tcagcaagcc catgctgtgc
    62341 cagtacggac tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc
    62401 ttcgttaaac aacgccgcca ggcgatcgtc acccagagtg gcgatatccg cgtcaatgcc
    62461 acaatgacca gcaaaggcca tttccgccag cgttaccagc agaccgccat cagagcggtc
    62521 gtgatacgcc agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt
    62581 cagttgcgcg acgtcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc
    62641 cagcgccgtt gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt
    62701 atcttcggta gaaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc
    62761 ggaaatcacc agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg
    62821 ggttttcatc gacatagagt ctttacccac cgggatcgta agacccagcg ccggacaaag
    62881 ctcttcaccc acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc
    62941 cgccgccatc cagttggcgg aaagtttaat gcgtttgata tcaccaatct gtgttgcggc
    63001 aatattggtt aacgcttcac cggccgccag acgggcagag gcggcgaaat ccagcagcgc
    63061 aaccggcgca cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt
    63121 agtgaccgcg cagttagcga ccggcacctg ccacggcccc accatctgat cgcgcgctac
    63181 catgccggtt acgctgcggt cgccaatggt caccaggaag gttttttccg ccacagtcgg
    63241 cagatgcagc acacgtttca ccgcatctgc aatggtgatc ccttcacggg ccagcgcgtc
    63301 gcctttcgct ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac
    63361 gtccagcggc agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag
    63421 ttcttcggtc gcttcaccaa tcaccgcgta gggcgcgcgt tcacgcttac acagttcgtc
    63481 aaacagcggt aactgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca
    63541 ccagatttcc agcgggctca tgcccggttc gtcgttcaga atatcgcgca gttcgaattt
    63601 accgccgcgt ccgccgtcgc tcaccagttc cggcatcgcg ttagaaagac cgcctgcgcc
    63661 gacgtcgtgg ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac
    63721 ttcctggcag cgacgctcca tctccgggtt gtcgcgctga acagaagcaa agtcgaggtc
    63781 ggcatcagac tgaccagacg ccatagaaga cgccgcgccg ccgccaagac cgatgttcat
    63841 cgccgggccg ccgagaacga ccagtttcgc accaacgttg atctcgcctt tttgtacgtg
    63901 atcggcacga atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag
    63961 ctcttcgccg ttgtggctgt tcactttttc ttcataagta cggaagtagc cgttcagtgc
    64021 cggacgacca aattcgttgt taaacgccgc accgcccagc gggccttcgg tcatgatgtc
    64081 cagcgcggtg acaatgcgct caggcttacc gaaatcttct tcccacggct gttcgaagcc
    64141 aggaattcgc aggttggaaa cggagaaacc aaccaaaccc gctttcggct tcgcgccgcg
    64201 cccggtggca ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg
    64261 agaaatcgcc gtcgggtggt tgtgagtttc gactttcatc agaatatgcg ccggttcctg
    64321 atggaaatcg tagcggcccg tttcgtggtc agcaaagtag cggcccactt cagaaccttc
    64381 cattacggcg gcgttatctt tataagcaga gagaacgtaa tctggcgtgg tttcgaaggt
    64441 gtttttgatc atcttaaaca gcgatttcgg ctgttgctca ccgtcgataa cccaatcggc
    64501 gttaaaaatc ttgtggcggc agtgctcgga gttcgcctgg gcgaacatat acagctcgat
    64561 gtcgttcggg ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa tttcatcttc
    64621 cgccagtgcc aggccaagac gcaggttagc gtcgattagc gcctgacggc cctgccccag
    64681 caaatcaacg ctggtgaccg gagtcggttg atggtgtgca aacagctgct ctgcatcatc
    64741 taaagcaaaa aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca
    64801 ttgttcattg gtcagcgtac cggcttctat atagtaagca acgccgcgct caaggcggtt
    64861 tacctgttgt agcccgcagt tatgggcaat atcggtcgct ttcgaagacc agggagagat
    64921 ggtgccagga cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc
    64981 cggaccatat ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt
    65041 gaggtcagca aaatggacat actcggcgta aatattgtga accgggagcc tggcagcctg
    65101 aaaacgtgcc agcagtttgt tgattcggaa tgccgacagt gcaggcgaac cacgcagaat
    65161 ttccatcata agtctctcgt cttctaagct ttcggtgtac ccaagggggg gaaacgggcg
    65221 tcattataaa gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg
    65281 tgaattagca acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg
    65341 caaaatgccg ctcatttatg agtaaacctt tcactattat tacgtttttt caagctggga
    65401 cgcgcacgac acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg
    65461 gcattctggc actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag
    65521 ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata
    65581 ctcccctgac ttataacgaa atcaacggga aaccttttgg cctggattac gaactggcga
    65641 aacagtttgc cgattacctc ggtgtaaaac tgaaagtgac cgtgcggcag aatatcagcc
    65701 agctgtttga cgacctcgat aatggtaacg ccgacctgct ggcggcagga ctggtctata
    65761 acagtgagcg ggtaaaaaat tatcagcctg gccctaccta ttattccgtg tcacaacaac
    65821 tggtttataa agtgggtcag tatcgcccgc gcacactggg caacctgacg gcggagcaac
    65881 tcaccgttgc accgggtcac gtggtggtta acgatctcca aaccctgaaa gacacaaaat
    65941 tcccggaatt aagctggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg
    66001 tcatcgaagg aaaactcgat tacaccattg ctgattctgt cgccatcagc ctgtttcagc
    66061 gcgttcaccc ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg gtgacctggt
    66121 ttagcccgtt agatggcgat aatacccttt ccgccgccct gctcgacttc ttcaacgaaa
    66181 tgaatgaaga cggtacgctg gcacgcattg aagagaaata cctagggcat ggcgatgatt
    66241 ttgattacgt cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa
    66301 agcccctgtt tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt
    66361 atcaggaatc gcactgggat gcacaggcca cttcaccgac gggtgtgcgc ggcatgatga
    66421 tgttaaccaa aaataccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga
    66481 gcatcagcgg tggcgtgcgt tatttgcagg atatgatgag taaagtgccg gaaagtgtgc
    66541 cggagaacga gcggatctgg tttgccctcg ctgcgtacaa tatgggctat gcgcatatgc
    66601 tggatgcccg cgccctgacg gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa
    66661 aacagcgtct gcctttactt agccagaaac cctattacag caagctgact tacggctacg
    66721 ctcgtggaca tgaagcctac gcttatgtcg aaaatattcg taagtatcag attagcctgg
    66781 tgggttatct gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg
    66841 attatccggc ggtatcgcct acggagttgg gcaaagagaa atttcctttt ctctcgtttc
    66901 tttcccagtc gtcatcaaac tatttgaccc attctccctc tctgctgttt tccaggaaag
    66961 ggagtgaaga gaaacaaaat taatccgtcg aggattgcgc ttttttctgc gctttaattt
    67021 cctggcggcg catgcgaaag aagtcactga gcaacgccgc gcactcatcc gccagtattc
    67081 cttccgtaat ttccactcgg tgattcatac ccggatgatg cagcacatcc attaaagatc
    67141 ccgcagcgcc agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga
    67201 tcatcgctcc ggcacacatt acacatggtt caagcgtgac atacaacgtg gcgtcgatca
    67261 gacgataatt ttgcatcacc agaccaccct gccgcagggc catgatttct gcatgtgcgg
    67321 tgggatcatg gcgaccaatc gggcggttcc agccttcgcc gattacccga ttgttatgca
    67381 ctaataccgc gccgaccggc acttcccgct catcccaggc acgtttcgcc agcgtcagcg
    67441 cgtgacgcat ccagtattcg tggctaaatt cgacttcaga caaaaagaaa actccggtta
    67501 taaaagcgcg gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt
    67561 caccgcgcgg ggttactcgc caacgatgct gacagaaata aagcagcgga ttgtcctgat
    67621 tactgtcgct atagccactg tacagccgca gcggagtgcc gattttgcgc tccagttgtg
    67681 cgaccttttc atgtcccaga caacgcatcg tcaataccca accaccatag ccacgctgaa
    67741 tttggctggc gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa
    67801 ccagcggctg cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg
    67861 tggttaatcg ctcctgaacc agcggaaagg cggtaacatt gtcgcgaaac cagcgcacga
    67921 aatcggcctg caacgtctgt aaacgtgctt cgctgtgacc aaaagtgcac ccccacagaa
    67981 gcagactcat cggccagcgt gccgcacgac cttttatcaa taacgcaatg gctataatcg
    68041 gtaacaacgg caggacaagt aacgcattca gcggttggcg acgcagtaaa tagcgcagaa
    68101 aactgccgaa catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac
    68161 gctcgtgagt tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac
    68221 tacatagcct aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa
    68281 ctggaatttt taattcatgg caattagcgg caatggaata taaaattcac tcgcgtgtct
    68341 ctcatattgc ctgttgtcgc cactatttca tcgcgaggaa agatatgaac ggcttacttc
    68401 gtatccgtca gcgttaccag gggcttgccc agagtgataa aaaactggcg gattatctgc
    68461 tgctacaacc tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag
    68521 tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc
    68581 ttaagttggc gttgagtgaa gcgctggcaa gccagccgga atcgccctcc gtgcccattc
    68641 ataaccaaat ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa
    68701 atactgccgc tatgtacgca acgctaaacg ttaatagtga agagaaactg catgaatgcg
    68761 taacaatgtt gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg
    68821 tggcgcaaaa ctttgcctgg aagctgatga agattggctt caatgctgcc gcagtgcgcg
    68881 atatgcatgc gctactcgca acagtacagg cgtcgtcccc tgacgatctg ttattagcca
    68941 tttcctacac cggtgtacga cgcgagttaa acctggcggc agatgagatg ctgcgagtgg
    69001 gcggaaaagt gctggcgatt accggcttta ctccgaatgc cctgcaacag cgtgcttctc
    69061 attgcctgta taccattgcc gaagaacaag cgacaaacag cgcttcaatc tctgcttgtc
    69121 acgctcaggg aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac
    69181 tggcaccaga acgtattcgt catagtgaag cgctggtgaa aaaactggtc tgagtaaaga
    69241 atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgttgtta
    69301 atcactaaaa aatgcatcaa ttgtgatatg tgtgaacccg aatgcccgaa tgaggcgatt
    69361 tcaatgggag atcatatcta cgagattaac agcgataagt gtaccgaatg cgtagggcac
    69421 tacgagacac caacctgcca gaaggtgtgc ccgatcccca atactattgt gaaagatccg
    69481 gcgcatgtcg agacagaaga acagttgtgg gataaatttg tgctgatgca ccacgcggat
    69541 aaaatttaat attctactct ggaagtagag tattaattat attactggga agccttaacg
    69601 ccattatatt tatttaattg atgacattag cataatcatt cactaagtta atttatatag
    69661 tatctgccaa gacacttatt tatagttatt aaaggcgcgt ccgattggtt caccggacgc
    69721 accttaagta cgtttccttg tgttataaga acagaaggat cagctgtaaa acagcaatga
    69781 tgattttgat gacccgttta atcaggtatc ggcaatcagt cattcgtttt tccttaaaca
    69841 aggaatgcag ctgttcagat tagcccttac atctccccaa aactgaacgt gcgagttatt
    69901 gagggtgcat gctgcactcc acaccagagc tttgacgaca ccactcgttt caatggggga
    69961 attctgtggc atggtgtaaa gcacagcaaa atcttcaata acgaagccaa ttttaatgta
    70021 cttacgaatt ggcggtcacg ttaatctttc catcaatatt gctttcttcg taaaggctcg
    70081 agtttttatg ctaaagattg caagttgctt gtaaaagata agtacactga tccataatcg
    70141 ctgttgttga gggtgcatgc tgcacaaaat taaagttaaa aagtaaaacc cccgttcctt
    70201 accagttcgg gggttttact ttttaaagag aacggtatta ttttaacttt caataattac
    70261 cgtggcacaa gcatagtggc gttcatctgc cagcgttaca tgcatattta caacgccgag
    70321 cttttccgcc agttttaatg cctcacccca tagccgtagc cgtggtttgc cgagctcatc
    70381 attgaatact tcaaattgat taaacgccag accattgcgg atcccggtgc caaacgcttt
    70441 tgctgcggct tctttcacag caaaacgctt cgccagaaaa cgcaccggct ggtggtgcgt
    70501 tttccagatt gcccattcgt tatcgcttaa tacgcggcgt gccaggcgct caccggatcg
    70561 ggcgatcact gcttctatgc gagcgatctc tacgatatcc gtgcctaaac ctaatattgc
    70621 cattagccac gcgcttccag catcagacgc ttcatttctg ccaccgcatc tttcagtccg
    70681 gtcatcactg cacgaccaat aatggcatga ccgatattca gttcatgcat ctcagggatg
    70741 gcggcaatag ctttcacgtt gtgataggtc agaccgtgac cagcgttaac tttcagaccg
    70801 agactagcgg caaaggtcgc ggctttggcg atacgcgcca gctcttgcgc ctgttcggcg
    70861 tcagttttgg catcagcata gcaaccggtg tggatctcga taaacggtgc gccaacctct
    70921 gccgcagctt tgatctgctc ttcatcggcg tcgataaaga gagaaacctg gatcccggca
    70981 tctgccagac gtttgcaggc atcgcgcatt ttgtcacgct gccctgcgac gtccaggccg
    71041 ccttcggttg ttacttcctg acgcttttcc ggtaccagac agcaaaaatg tggcttcgtc
    71101 tcaacggcga tcgccagcat ctcttcggtc accgccatct ccagattcat gcgggtatcc
    71161 agcgtctgac gcaggatgcg cacatcacgg tcagtaatgt ggcggcgatc ttcacgtaaa
    71221 tgcacggtaa tgccgtccgc tcccgcctgc tcagcaataa acgccgcctg caccggatcc
    71281 gggtaagcag taccgcgtgc gttacgcagc gttgcaatat ggtcaatgtt gacgcctaac
    71341 agtaattcag ccatgacaat cctcatcatt cataatgtgt tttcaccgtt cgcttaggca
    71401 taaactgccg gaacagttcc ctgctcttta atggtttacc gccaagatac ggcttaagcg
    71461 ccatgcgggt aaagcgtttc gcggcgcgta gtgtgtctgc gtcaggaaat tcccgcgcgt
    71521 ttaacgcttt taactgcctt ccggtgaacg ttttattgtc gataacgacg cttgcgataa
    71581 accctttttc ttcgcgataa cgatacgtca tggtgtcatc taccggctcg ccgctacccg
    71641 cacaatgggt aaaattgacg ccatagccca gatgcccgag cagtgccagt tcaaagcggc
    71701 gcagcgcggg ttctggcgta ccagtggcac ctgcaagaga ctgaatgcag tgcaagtaat
    71761 cgaaaaagag ttcagagaag cgcgtctcgt attccagtac gcgggagaga agttcgttga
    71821 tgtacagacc gctgtaaagc gtgataccgc ttaatggcag cgccagcgag acagcttcag
    71881 cactgcgcag cgttttgact tcgccacgcc cgccgaaacg tagcaagaga ggggtgaaag
    71941 gctgtaatgc acctttcaga gtagagcgtt tagagcgtgc gcctttggca accagacgca
    72001 cgcgacccga ttcctccgtg aagacgtcca gcatcaggct ggtttcgctc cacgggcgac
    72061 tatgcaggac aaatgcgcgc tgccagcctt ccatcggagt gactcttaaa gatcgtcaac
    72121 gtaaccgaga ctgcgcagtg cgcgttcgtc gtcggcccaa ccggatttca cttttaccca
    72181 cagttcaagg tggacaggcg cttcgaacat ttcctgcatg tctttacgcg cttcaatccc
    72241 gatggttttg atcttggccc ctttgttgcc aataaccatc ttcttctgcc cttcacgctc
    72301 aacgagaatc aaaccattga tgtcataacc accgcgttcg ttagagacga aacgttcgat
    72361 ctccacggtc acggagtacg gcagttcggc gccgaggaaa cgcatcagtt tttcgcggat
    72421 gatttcagac gccataaaac gctgtgagcg atcggtgatg taatcttccg ggaagtgatg
    72481 ggtcgcttca gggagatgct tacgcacgat tgctgcgata gtgtcgacat tcagcccggt
    72541 ttcagcagag atcggcacga tatcgaggaa gttcatctga cttgccagga actgtaagtg
    72601 cggcagcaga tcggcttttt cctgcacgtt atccactttg ttcaccgcga ggatcaccgg
    72661 cgctttgcca tcgcgcagtt tgttgagcac catttcgtcg tccggcgtcc agcgggtgcc
    72721 ttcaacgaca aaaatcacca gctcaacgtc gccaatagag ctgctcgccg ctttgttcat
    72781 caggcggtta atagcgcgtt tttcttccat atgcaggcca ggggtatcaa cgtagatcgc
    72841 ctgatacgcg ccttcagtat ggatccccac aatgcggtga cgagttgttt gcgctttgcg
    72901 ggaggtgatg gagatcttct gccccagcag tttgttcaac aatgtggatt tgccaacgtt
    72961 cggacgtccg acgatggcaa taaatccgca gtaactttta tcgatgctca ttccagctcc
    73021 agttttttca acgcctgttc ggcggcagcc tgctcagcct tacgacggct tgaacctgtg
    73081 ccaaccaccg gttcactcag gccgctgacc tggcagtgga tagtaaattc ctgatcgtgc
    73141 gcttcgccac gtacctggac taccagataa gtcggcagcg gcagatggcg accctgcaaa
    73201 tattcttgca agcgcgtttt cggatctttt tgtttatcgc ctgggctaat ttcgtccaaa
    73261 cgagtttgat accagttgag gattaatttc tcgacggttt gaatatcact gtcgaggaat
    73321 acgccaccaa ttaatgcttc gacggtgtcg gcgagaattg actcacgacg aaatccaccg
    73381 cttttaagtt cacctggccc taaacgtaag cactcgccta actcaaattc gcgcgccagt
    73441 tccgccagcg tattgccacg gaccagcgtg gcgcgcatcc ggctcatatc gccttcatcc
    73501 acacgaggga aacggtgata aagcgcattg gcgataacgt agctcagaat agagtcgcct
    73561 aaaaattcta aacgctcgtt atgtttgctg ctggcactac gatgagttaa tgcctgctgc
    73621 aacagttcct gatgattaaa agtgtagccc agcttccgtt gaagccgatt aattacgatg
    73681 gggttcatgc gataccaata aataaatgcg tcaacaattc agcacacgaa acagacctga
    73741 tatacatggc tctgctaact gcttcgctgc agtttcagtt taccggtata tggggaccaa
    73801 cgctgtttcg tgtgccgtgg caacctggag gtgccaacct taaacttcgg gggaatattc
    73861 tatacacaac gacgggggat gtcgttagcc acgggagatt tatctcataa ataattcacg
    73921 ttgtcgccat aacggcgaca acgtgaacga agatgggcta ttaatggatg ccgccaatgc
    73981 gacttaagcg cacacctgtc ggccattcgc cttcttgctt atcgaaactc atccagatag
    74041 ccgttgcccg accgaccaga ttcgcttcag gcacaaaccc ccaataacgg ctgtccgcgc
    74101 tgttgtcgcg gttgtcgccc atcatgaagt attgtcccgg aggaacaatc caggttgcca
    74161 gttgttgccc tggctgctgg taatacatcc ccacctgatc ctgcgcaatc ggcactgtca
    74221 gaattcggtg cgtcacatca cccagtgtct ctttacgctc ggaaagacga attccatttt
    74281 ctttggtttc gtttttcggc acttcaaaga atccgctggt cgcttcccca ccattacggc
    74341 gtgagaaggt ctgaacgaaa tcgctcggtt ccacgtttga gtaggtgacc ggcagcgcgt
    74401 tttcacacgc ctggccggaa ctgcatcccg gttgaatcgt cagctctttc gagaccggat
    74461 cataagtaac tttatcgccc ggtaaaccca ccgcgcgctt gatgtaatca agctttggat
    74521 cttccggata tttaaagacc acgatatctc cgcgtttcgg atgaccggtt tcgatcagcg
    74581 ttttctggta gataggatct ttaatgccat aagcaaactt ttctaccaga ataaaatcac
    74641 cgattaacag agtcggcatc atcgaacctg acgggatctg gaacggttca taaataaacg
    74701 aacgcacaat caatacgata gccagtaccg gaaaaacaga agcaccggtt tccagccagc
    74761 caggcttcgg cgcaaccttt ttcaacgttg ctttatccag tgagtcaccg gcagccgcct
    74821 gcgccgctgc ctgacgttcc cgccgtttag gtgcgaaaaa gaatttatcc acgcaccata
    74881 aaatgcccgt caccagtgtg gcaatcacca gaatcagggc aaacatattc gccatgccaa
    74941 ctcctaaggg ttatttgttg tctttgccga cgtgcagaat ggcgaggaac gcttcctgcg
    75001 gcagctcgac gttaccgatc tgcttcatgc gtttcttacc ttctttctgc ttctgcagca
    75061 gctttttctt acggctgata tcgccgccat aacatttagc cagtacgttt ttacgcagct
    75121 gtttcacggt ggatcgcgca ataatgtgcg taccaatcgc tgcctgaatg gcgatatcaa
    75181 actgctggcg tgggatcaga tctttcatct tctccaccaa ctcgcgaccg cggttttgcg
    75241 aattatcacg gtgggtgatc agcgccaacg catcaacacg ttcaccgttg attaatacgt
    75301 ctacacgtac catgtcggac gcctggaagc gtttgaagtt gtaatccaga gacgcataac
    75361 cacgcgaggt agatttcagg cgatcgaaga agtcgagcac cacttccgcc atcgggatct
    75421 cgtacgtcag cgccacctga ttaccgtggt aaaccatatt ggtctgcacg ccgcgttttt
    75481 ctacgcacag cgtaataacg ttgccgagat atgcctgcgg cagcagcatg tgacattcgg
    75541 caatcggctc gcgcagttcg tagatgttat ttaccgctgg cagcttggat ggactgtcga
    75601 cgtaaataac ttctcttgac gtggtttcaa cttcatacac tacggtcggc gcagtggtga
    75661 tcagatccag atcgtattca cgttccagac gttcctggat gatctccatg tgcagcaggc
    75721 cgaggaagcc gcagcggaaa ccaaagccca gcgcgctgga gctttccggc tcatagaaca
    75781 gtgaagcatc gttcaggctg agtttaccca gcgcgtcacg gaaggcttca tagtcgtcgg
    75841 aacttaccgg gaacagaccg gcgtatacct gcggtttgac tttcttaaag ccaggcagcg
    75901 ccttttctgc cggattacgc gccagcgtta aggtatcgcc gactggagcg ccgtggatat
    75961 ctttaatcgc acataccagc cagcctactt cgccacattt cagttcagtg cggtcaacct
    76021 gtttcggcgt gaagatgccc agacggtcgg cgttataggt ctgcccggta ctcatgactt
    76081 tcactttgtc gcccttacgc agggtgccgt ttttaatacg gataagtgaa acaacgccca
    76141 ggtagttgtc gaaccatgag tcgataatta gtgcctgcaa cgggccttcc ggatcgcctt
    76201 ccggcggcgg aatgtcgcgc accagacgtt cgagaacgtc ctgcacgcca acgccggttt
    76261 tcgctgaaca gcgcaccgcg tcagtggcgt cgatgccgac gatatcttca atttcttccg
    76321 ccacgcgttc aggatcggct gccggcaggt caatcttgtt cagtactggc accacttcga
    76381 gatccatttc catggcggtg tagcagtttg ccagggtttg cgcttccacg ccctgcccgg
    76441 cgtcgaccac cagcaatgca ccttcacagg cagccagcga acgggaaact tcataggaga
    76501 agtctacgtg gcccggggtg tcgataaagt taagctgata ggtttcgccg tcagacgctt
    76561 tgtagtccag cgtcacgctt tgcgctttga tggtaatgcc acgctcacgc tcaagatcca
    76621 tggaatcgag aacctgcgcc tccatttcac ggtcagacag gccaccgcag atctggataa
    76681 tacggtcaga cagcgtcgat ttaccgtggt caatgtgagc tatgatcgaa aagttacgta
    76741 tattcttcat aaagtatgat tattgtgcct taatgcccgg ttaaccaggc ttttagaagt
    76801 cgctgttctg agcttaacgt ctgtattaat agaaacgccg cattctacac tacaacattg
    76861 aggcgaggaa atgttcatac cgtatggatt gtggtatctg gaaacgtcct cgcatttgtt
    76921 atgcaaaatg caacaaagcc agtgaaatca ctggctcgcg tcttccgaag atgtttcaaa
    76981 tcgcacaagg ccaggcggca atgccacgct taagatgatc ggttgccatt ctgcccgggc
    77041 agcaaactta cgcgagtagc cgcgcgcaat caggaacccg ccaataccac cgaggatcgc
    77101 accacataat gctgcgacgt cagaagcaaa gagtagctga aatagcgaag cgataaggaa
    77161 taatcccacc agcggcgaca tataaaccag taatgcggag ctaagcaggc tgccttcggc
    77221 gatccccaat tccacttttt gccccggcac taacggttca tcacagggta cgacaatggt
    77281 atgcgtggtt tgcgggccaa gtttattcaa cacgcggcta ccgcaaccgg cgcgtgaagc
    77341 gcagctgctg catgaagctt taacatcaca actgaccagc gcctgcccgt tttgccagga
    77401 gacgacggta gcccactctt tgatcattgc gctgccccga acttaatatt ctcggcaatg
    77461 cgtttcgccg tttgcggcgg cagttcaccg acaatggtga tttcggcgtt atcacgtacg
    77521 cttgtactga cggttctgcg tccggtgcgc aacatctgat cggtgctcga tggcgtagcg
    77581 cggttaacgt ttaccgagaa gctgaataat ccgtcggaat agagacgtga ttcgataggc
    77641 atgttgtcca tcgtcggtag cggacgtcga ctactggaaa cttcgctaaa accctgtggc
    77701 aaccaggttg gcgtccagct gaatttagct ttttcaccta caggaacaga aagcaacggc
    77761 ggcaaatttg ccttcgccag cgtctgcata ctgctactga tatcctgatt gacgttaaaa
    77821 gcaatcacgc gaaattgttc cagcgtttca ccatcgcgat caaggagatc aaccctcatc
    77881 ggtaatttcg attcgatgtc catccacacg atgtagctgt agcgtgtacc atctcgggca
    77941 accacgcgaa tgacttcgca aagacgatca gcaatacgcg tgcgcccgac ggagataaag
    78001 tcgtagtaag gagaaaggcg tttgaaatcg gtatagatga gcgatggcag agaatcaaca
    78061 atgtaatcgc cattaagcgt gaacggttca agtcccggtt caaaatagct gatttcattg
    78121 ccacgctgta ccacttcccg gcgcgggcca tccatttgca acaattgtgc aagaggacgg
    78181 ttatcgaggc gtgcatgtcg ataacgcaga gactcaacac cctgtttatt gatgctgatg
    78241 aatgacagct cgtaattcag tgactgactg gccaggttca tctgctgtaa taacgccccg
    78301 gacgcgggag tggccgaggc gttagcagag aataacaggc tacctgtcac taatgacatg
    78361 gcaaaccaaa gttgcttcat tactgcgatt gcgttcctaa agtttgaatt cctggcacct
    78421 gtacagcggc ttgctgggtt tgcgcctgct caaactgaag ctgttcagag tggagtcggc
    78481 gttgcagttc gtaatcctgc aacattgcat taatgcgacg acgctgctcc tgtacctgct
    78541 gctgttgacc attgtttgcg gtcgcttcag aaggtactcc caggcttacc gggctggctt
    78601 tacccatcat cggcagtgta ttaaataccg gcgtttcggg ctgctgggac gtttcagatt
    78661 gtccattata gtgctggacg ccaacgataa ctgcaagcga tacgcatgct gctacgccca
    78721 tttgggtaag ctgtgccgcc cacggacgta ctttctgcca gaatggcatt ttctgccatt
    78781 gatgcggcgc aggctgggct tccgggatca atgtcgccgg ttgacgtact ggctcttctt
    78841 caatggcggc catcacgcgt gaagagatat cgaaatggag cacctcggga gtatcacccc
    78901 gcattgagtc acggattaag tgatagcttt cccaggtttt ctgcatttct gggttatgag
    78961 ccagttcgtt aagcagctca ctatccagcg tttcgccatc cattaaagcg gaaagttgtt
    79021 ctttctgcat gcctaatacc cttatccagt atcccgctat cgtcaacgcc tgataagcgg
    79081 ttgaactttg ttatcaatag cttccctcgc tcggaagata cgtgaacgca ccgtacctac
    79141 cggacaatcc atgatagcgg ctatctcttc atagctcagg ccatccagct cccgcaaggt
    79201 tattgccatg cgcaaatctt ccgggaggga ctcaatagtt cggaaaacta tctgtctcag
    79261 ttcttctgac aacattaagt tctcagggtt cgaaatttct ttcaacgcgc cgccactttc
    79321 gaagttttca gcttcaatgg catccacatc actggaaggt ggacgacgcc cctgagcaac
    79381 caggtaattt ttcgctgtat ttacagcaat ccgatacagc catgtataaa aagcgctatc
    79441 tccccggaac gaatccagcg cacgataggc tttaataaaa gcttcttgta ccacatcggg
    79501 aacatcaccc gacggcacat agcgggaaac cagactcgcc actttatgct gatagcgcac
    79561 taccagtagg ttaaaggctt tctgatctcc cttctggacc cgttcaacca ggacctggtc
    79621 cgttaactgc tcgctcatcc gaggtaaagt ctccccaaac caaatttcca cgcgctatcg
    79681 aaacgccact ccattagctg caatttgagc aagcaaaggg ttagagtgtc tcgtttttgt
    79741 aaagttccgt aacgcatctg tttttgtttg tcatgctgta gacggatcat tatctatcat
    79801 tataagtcta cagaatctga acatcgcatt atctgtgtag aaatgcccat ttaactgcct
    79861 gaagagtaac ccaacggcct ttttatttca ccacctaatc ctccaccagc cagtaacttc
    79921 tctttttctc gccgccctgc gtcagcgtgt ttagccactg taacaaatat taaaatagca
    79981 ggtgtttatt cgcacaacat gatgctatgc tgaccaaacg gtgtttagta aattaaacaa
    80041 agaaaatgaa tactctccca gaacattcat gtgacgtgtt gattatcggt agcggcgcag
    80101 ccggactttc actggcgcta cgcctggctg accagcatca ggtcatcgtt ctaagtaaag
    80161 gcccggtaac ggaaggttca acattttatg cccagggcgg tattgccgcc gtgtttgatg
    80221 aaactgacag cattgactcg catgtggaag acacattgat tgccggggct ggtatttgcg
    80281 atcgccatgc agttgaattt gtcgccagca atgcacgatc ctgtgtgcaa tggttaattg
    80341 accaaggggt tttgtttgat acccacgttc aaccgaatgg cgaagaaagc taccatctga
    80401 cccgtgaagg tggacatagt caccgtcgta ttcttcatgc cgccgacgcc accggtagag
    80461 aagtacaatc cacgctggtg agcaaggcgc agaaccatcc gaatattcgt gtgctggagc
    80521 gcagcaacgc ggttgatctg attgtctctg acaaaattgg cctgccgggc acgcgacggg
    80581 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
    80641 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
    80701 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
    80761 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
    80821 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
    80881 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
    80941 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
    81001 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
    81061 tcacacaaga accgataccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
    81121 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
    81181 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
    81241 gctggtcggc ggcagaagat atcaccagac gtatgcctta tgcccacggc gtcagtacgt
    81301 taccgccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
    81361 actggcacga gctacggctg tttatgtggg attacgttgg cattgtgcgc acaacgaagc
    81421 gcctggaacg cgccctgcgg cggataacca tgctccaaca agaaatagac gaatattacg
    81481 cccatttccg cgtctcaaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
    81541 tgattgttcg ctgtgcgatg atgcgcaagg agagtcgtgg cctgcatttc acgctggatt
    81601 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
    81661 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
    81721 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
    81781 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
    81841 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
    81901 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
    81961 tggtatagcg agcctgttcc cgttgagggg tcgcgcactc tactccctgc tgatagtaag
    82021 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgatccac tgctgaatat
    82081 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
    82141 gcgcggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca tcggttcgct
    82201 gcgccagcat taatgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
    82261 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
    82321 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
    82381 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
    82441 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
    82501 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
    82561 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
    82621 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
    82681 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
    82741 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
    82801 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat caccggcggc
    82861 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
    82921 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
    82981 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
    83041 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
    83101 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
    83161 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
    83221 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
    83281 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
    83341 aacaactgct atctcgaagg tgagatggta caaggcaagc gtaacgaagc gatcaagcgt
    83401 ttgaccgaag gtcgcgtaaa cgtactggtt gcaaccgatg ttgccgcgcg cggtatcgac
    83461 attcctgacg tcagccacgt ctttaacttc gatatgccgc gcagtggcga tacttatttg
    83521 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
    83581 gcccatgacc atttgctgct gggcaaagtt ggccgttata ttgaagagcc aattaaagct
    83641 cgcgttattg atgagttacg cccgaaaacg cgtgcgccaa gcgagaagca gaccggcaag
    83701 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagttaaaga gaaagagaag
    83761 ccgcgagtga aaaaacgcca tcgcgatacc aaaaatattg gtaagcgccg taaaccaagc
    83821 ggaacgggcg tgccaccgca aacgacagaa gagtaatctc aatgccgggt ttaagcccgg
    83881 cattattttt atccagccac aaaacactct tcaacacact gaataaaagt gtgcatcgcc
    83941 ggactaaccg cttttccggc atggtgagcg cacattgccg taatggtctg cgtctgttcg
    84001 ccaaaaggca attcgattaa ttcgccactc gcaagttcat ttcccactgc aaagcgcggc
    84061 aggtaactga ccccgatatt tgccgcgaca caacgcttga tgctttcgat acttataagc
    84121 tcaatggtgt tttccaccgt gatccgccgc tggcgcagcg tgctctcaaa tatctgccgg
    84181 aagacacatt gtggttcgtt gataataaag ctacaggcgt tatgtcttcc cggctcagta
    84241 aaatcgatat ctgcaatttg cggtgaagcc accagcacca gcggttgttc acccaactct
    84301 cgtcggttca gaacatcatc attccctacg cgataaaaaa ccccgacatc ggcctcatca
    84361 ttcagtagcg catcacggat cacgtaacag ttcagcgact gcaacgataa acgcactttt
    84421 ggagcgcgtt gccgaaaacg ctgcaacacc tgcggcattc ggtaagagag caacgtttcg
    84481 cctgaaacaa cgcgtagttc cccgtccgga tccgactctt ttttcgcagc ctcacgaagc
    84541 gtatccatca ctcgggtaag ttcatagatg tgcggcagca acttttttcc ttcgcgggta
    84601 aggcacattc gtcgaccaat tttctcaaac aattgtaccg agaactcctg ctcaagctgc
    84661 tgaatatgaa aagtcaccgt cgattgtgta cagcacaatt tttgcgaagc tcgcaaaaag
    84721 gaaccctctt ccaccacggt tttaagtgta ataaaacggc gcagatccat aaccccaaac
    84781 ctatcgaaaa tatcgaattt agaatgtaaa aacattcatt tttttaaatg ttccgtgtcg
    84841 ggtactgtct accaaaacag aggagataac aagtgacacc gaccctttta agtgcttttt
    84901 ggacttacac cctgattacc gccatgacgc caggaccgaa caatattctc gcccttagct
    84961 ctgctacgtc gcatggattt cgtcaaagta cccgcgtgct ggcagggatg agtctgggat
    85021 ttttgattgt gatgttactg tgtgcgggca tttcattttc actggcagtg attgacccgg
    85081 cagcggtaca ccttttgagt tgggcggggg cggcatatat tgtctggctg gcgtggaaaa
    85141 tcgccaccag cccaacaaag gaagacggac ttcaggcaaa accaatcagc ttttgggcca
    85201 gctttgcttt gcagtttgtg aacgtcaaaa tcattttgta cggtgttacg gcactgtcga
    85261 cgtttgttct gccgcaaaca caggcgctaa gctgggtagt tggcgtcagc gttttgctgg
    85321 cgatgattgg gacgtttggt aatgtgtgct gggcgctggc ggggcatctg tttcagcgat
    85381 tgtttcgcca gtatggtcgc cagttaaata tcgtgctcgc gctgctgctg atctattgcg
    85441 cggtacgcat tttctattaa ttaaaaaagc ggaagaggtc gccctcttcc gcttagtaac
    85501 ttgctactta agccttacag gctttcagta aaggtacgag cgataacgtc gcgctgctgt
    85561 tccggagtca gagagttaaa gcgaactgca taacctgata cacggatggt cagctgcgga
    85621 tatttttccg gatgcttaac tgcatcttcc agagtttcac gacgcagaac gttaacgttc
    85681 aggtgttgac caccttcaac gcgaacttct ggtttcactt ctactggaac ttcacggtat
    85741 tcaatgtcac ccagtttgct tactgcaacc acttcatctt ctgcataacc tgcttttgca
    85801 acgatgcaac gcgcttcgcc tttttcgctg tccagcagcc agaaagagtt cagcagatcg
    85861 tcgttagcgg ctttagtaat ctggatacct gtaatcatgt gatgcctccc cggcaaaatt
    85921 atttgattta ttcagcctgt cgcggccaat tggtaaaacc attgttgctt gagtgtatat
    85981 atactcctca acaacccttg attccttgat ttaaatcaat aaaaaccaca catcgattgt
    86041 ggtcgcaaat ggattttatt gttttacatc aacttatgcg ggtgtgaaat tttaccaatt
    86101 tacatttttt tgcactcgtt taagtctaaa aaatgagcat gattttgttc tgtagaaata
    86161 agcagttaag ctaggcggat tgaagattcg caggagagcg agatggctaa cgaattaacc
    86221 tggcatgacg tgctggctga agagaagaag caaccctatt ttcttaatac ccttcagacc
    86281 gtcgccagcg agcggcagtc cggcgtcact atctatccac cacaaaaaga tgtctttaac
    86341 gcgttccgct ttaccgagtt gggtgacgtt aaagtggtga ttctcggcca ggatccttat
    86401 cacggaccgg gacaggcgca tggtctggca ttttccgttc gtcccggcat tgccattcct
    86461 ccgtcattat tgaatatgta taaagagctg gaaaatacta ttccgggctt cacccgccct
    86521 aatcatggct atctcgaaag ctgggcgcgt cagggcgttc tgctgctcaa tactgtgttg
    86581 acggttcgcg caggtcaggc gcattcccac gccagcctcg gttgggagac gtttactgat
    86641 aaagtcatca gcctgattaa ccagcaccgc gaaggcgtgg tgtttttgtt gtggggatca
    86701 catgcgcaaa agaaaggggc gattatagat aagcaacgcc atcatgtact gaaagcaccg
    86761 catccgtcgc cgctttcggc gcatcgtgga ttctttggct gcaaccattt tgtgctggca
    86821 aatcagtggc tggaacaacg tggcgagacg ccgattgact ggatgccagt attaccggca
    86881 gagagtgagt aaatttgcgg ggaaatgccg gatggcagag ttgccacccg gctgatttat
    86941 caggctttat tctgacgcca ccattcacca agcaaaacgc cggttgcgac agagatattc
    87001 agcccggcaa cgttgcccgt accgtcaatc ttcacgcgca gatcgttcgg atcgcgtgcg
    87061 gcatccggta acccttcata ttcctgaccc agcaccagta ccattttcgc tggcagacta
    87121 gttttgaaca gcggtttacc ctgctcgctg gaagtggtca ctacggtgta acctgcctga
    87181 cggaaatcat ccagcacgtt aacaatgttg tcgccagtaa tcggctgaac gtgctctgcg
    87241 ccgccttctg cggtacggat agccgccccc gactccagca gtgccgcatc ctgcaccacg
    87301 acacctttca cgccgaagtg cgcgcagctg cgcatcatgc cacccaggtt atgcgggtta
    87361 gattcgtttt ccagtgccag aacgcaatcc tgcgcgcctg cctggcttac ccactgctgc
    87421 acggttgtac cgttacgctt tttgatcaag aagcaaacgc cgccgtgatg ttccgtgcct
    87481 gacgcttttg tcaattccgc ttcatccacc acatggtacg ctttgcggtt tgctgccatc
    87541 cagcgcaagg cttctttaaa acgcggcgtt acactctgga taaaccaggc gcgaacaatc
    87601 gcttccggac ggctctggaa aagtgcctga catgcatttt cgccgtagac gcgggtttct
    87661 tccgcacgct gacgacgcaa cacttccgga tcaataaaac ttttaccact gatgccaccg
    87721 tgatcggcct tttccggcgt ctcatcaccc ggcgcgcggg aaaccgtgcg ccacggcgaa
    87781 tcttcccact tgcggtcacg gggctgactc tgtttgtcat cgcgggcggg gcgacggcca
    87841 ccgtcagcac gagattttcc tggacgcccg ccccctttcc cggtacgcgg gttgtgggta
    87901 cgtttatcag aatcatcatc actgcggaca tacatcactt tgaccttgcc gcttttacct
    87961 ttcatttcat cgttcatgct tttctccacc agcgctgcgc gaagcgcgca gattacccga
    88021 agtccgcgcg gttcgccatg atttcgtacc aaagcctgcg actatcatac ctattgaata
    88081 aaacagattg ttgtctggaa caatgtcccc gataatatgt aacatattag aaacataccg
    88141 gcgtcgttgc cgataagtct ccttactcat cccgaggtta gttatgaata ccgtttgtac
    88201 ccattgtcag gccatcaatc gcattcccga cgatcggatc gaagatgcgg caaaatgcgg
    88261 acgctgcggt cacgacttgt ttgacggaga ggtgattaat gcgaccggtg aaacactcga
    88321 caaattgctg aaggatgatc tacctgtggt gatcgacttc tgggcaccgt ggtgcggccc
    88381 ctgccgtaat ttcgcaccaa tttttgaaga tgtcgcgcaa gagcgtagcg gtaaagtgcg
    88441 ctttgtgaaa gtgaataccg aagctgaacg tgaattgagc agtcgctttg gaattcgtag
    88501 tataccgacg atcatgattt tcaaaaacgg tcaggttgtc gacatgctta atggcgcagt
    88561 accgaaagcg ccgttcgata gctggctgaa cgaatctctt taatcttacc ggggcacatc
    88621 ttgtgccccg ttttctcctc tgcgacaatg gcgttttttc gacgctctct tatgaccgaa
    88681 aacgctgttc tccagttacg cgccgagcgt attgcgcgcg caacacgtcc ttttcttgcc
    88741 cgcggtaatc gcgttcgtcg ctgccaacgc tgtcttttgc cagagaaatt atgtctctgt
    88801 tcgacaatta ccccagcaca agcaaaaagt cgcttctgtt tgctgatgtt cgacaccgag
    88861 ccaatgaagc caagtaacac cgggcgtctg attgctgata ttttgcctga taccgttgcg
    88921 tttcaatggt cgcgtaccga cccctcgcaa gacttgctgg atctggtaca aaacccggac
    88981 tatcaaccaa tggtggtctt tcccgcctcg tatgctgatg agcaacggga agtgatcttc
    89041 acaccacctg ccggtaagcc accgctgttt atcatgctcg atggtacctg gccggaagcg
    89101 cgcaagatgt ttcgtaaaag tccgtatctg gataatcttc ccgtcatttc cgtcgatctt
    89161 tcccggcttt ctgcctatcg cctgcgtgaa gcccaggctg aaggccaata ttgtactgcc
    89221 gaggtagcca tcgcactgtt agatatggcc ggcgataccg gggcggcagc agggttaggc
    89281 gagcttttta cccgctttaa aacacgctat ctggcaggaa aaacgcaaca tctgggtagc
    89341 atcacagcag aacagttaga aagcgtttaa aatcattcgg tcacttctgc gggagaccgg
    89401 tatgagtcag cgaggactgg aagcactact gcgaccaaaa tcgatagcgg taattggcgc
    89461 atcgatgaaa cccaatcgcg caggttacct gatgatgcgt aacctgctgg cgggaggctt
    89521 taacggaccg gtacttccgg tgacgccagc ctggaaagcg gtgttgggtg tgttagcctg
    89581 gccggatatt gccagcttgc cctttacacc cgaccttgcg gttttatgta ccaatgccag
    89641 ccgtaatctt gctcttctgg aagagctcgg cgagaaaggc tgtaaaacct gcattattct
    89701 ttccgccccg gcatcgcaac atgaagatct ccgcgcctgc gccctgcgcc ataacatgcg
    89761 cctgctagga ccaaacagtc tgggtttact ggctccctgg caaggtctga atgccagctt
    89821 ttcgcctgtg ccgattaaac gcggcaagct ggcgtttatt tcacaatcgg ctgccgtctc
    89881 caacaccatc ctcgactggg cgcaacagcg tgagatgggc ttttcctact ttattgcgct
    89941 cggcgacagc ctggatatcg acgttgatga attgctcgac tatcttgcgc gcgacagtaa
    90001 aaccagcgcc atcctgctct atctcgaaca gttaagcgac gcgcgacgct ttgtttcggc
    90061 ggcccgtagt gcctcgcgta ataaaccgat tctggtgatt aaaagcggac gtagcccggc
    90121 ggcacagcga ctgctcaaca cgacggcagg aatggatccg gcgtgggatg cggctattca
    90181 gcgtgccggt ttgttgcggg tacaggacac ccacgagctg ttttcggcgg tggaaaccct
    90241 tagccatatg cgcccgctgc gtggcgaccg gttgatgatt atcagcaacg gtgctgcgcc
    90301 tgccgcgctg gcgctggatg ccttatggtc acacaatggc aagctggcaa cgctaagcga
    90361 agaaacctgc cagaaactgc gcgatgcact gcctggacat gtggcaatct ctaacccgct
    90421 cgatctgcgc gatgacgcca gcagcgagca ttatgtcaaa acgctggaca ttctgctcca
    90481 cagccaggat tttgatgcgc tgatggttat tcattcaccc agcgcagcgg ctcccgcaac
    90541 agaaagcgcg caagaattaa ttgaagcggt aaagcatcat ccccgcagca aatatgtctc
    90601 tttgctgacg aactggtgcg gcgagcactc ctcgcaagag gcacgacgtt tattcagcga
    90661 agccgggctg ccgacctatc gcacgccgga aggcaccatc accgcgttta tgcatatggt
    90721 ggagtaccgg cgtaatcaga agcaactacg cgaaacgccg gcgttgccca gcaatctgac
    90781 ttccaatacc gcagaagcgc atcttctgtt gcaacaggcg attgccgaag gggctacgtc
    90841 gctcgatacc catgaagttc agcctatcct gcaagcgtat ggcatgaaca cgctccccac
    90901 ctggattgcc agcgatagca ccgaagcggt gcatattgcc gaacagattg gttatccggt
    90961 ggcgctgaaa ttgcgttcgc cggatattcc acataaatcg gaagttcagg gcgtaatgct
    91021 gtacctgcgt acagccaatg aagtccagca agcggcaaac gctattttcg atcgcgtaaa
    91081 aatggcatgg ccgcaggcgc ggatccacgg cctgttggtg caaagtatgg ctaaccgggc
    91141 tggcgctcag gagttgcggg ttgtggttga gcacgatccg gttttcgggc cgttgatcat
    91201 gctgggtgag ggaggtgtgg agtggcgtcc tgaagatcaa gccgtcgtcg cactgccgcc
    91261 gctgaacatg aacctggccc gctatctggt tattcagggg atcaaaagta aaaagattcg
    91321 tgcgcgcagt gcgctacgcc cattggatgt tgcaggcttg agccagcttc tggtgcaggt
    91381 ttccaacctg attgtcgatt gcccggaaat tcagcgtctg gatattcatc ctttgctggc
    91441 ttctggcagt gaatttaccg cgctggatgt cacgctggat atcgcgccgt ttgaaggcga
    91501 taacgagagt cggctggcag tgcgccctta tccgcatcag ctggaagaat gggtagaatt
    91561 gaaaaacggt gaacgctgct tgttccgccc gattttgcca gaagatgagc cacaacttca
    91621 gcaattcatt tcgcgagtca ccaaagaaga tctttattac cgctacttta gcgagatcaa
    91681 cgaatttact catgaagatt tagccaacat gacgcagatc gactacgatc gggaaatggc
    91741 gtttgtagcg gtacgacgta ttgatcaaac ggaagagatc ctcggcgtca cgcgtgcgat
    91801 ctccgaccct gataacatcg atgccgaatt tgccgtgctg gttcgctcgg atctcaaagg
    91861 gttaggctta ggtcgacgct taatggaaaa gttgattacc tatacgcgag atcacggact
    91921 acaacgtctg aatggtatta cgatgccaaa caatcgtggc atggtggcgc tagcccgcaa
    91981 gctcgggttt aacgttgata tccagctcga agaggggatc gttgggctta cgctaaatct
    92041 tgcccaacgc gaggaatcat gagtaaggta ctggaaatgt tgaccactta atcgggactg
    92101 gtgttattat tgcccgctta tgtcgtctgc attgcacaga ggacccttca atgaacagag
    92161 aagaaatgca ctgtgatgtt gtcaaaattt aagcgtaata aacatcaaca acaccttgcc
    92221 caactaccca agatttctca atcagttgat gatgtcgatt tcttttacgc tcccgccgac
    92281 ttccgggaga cgctgctgga aaaaatagcc agcgcgaagc agcgcatttg cattgtcgcc
    92341 ctgtatctcg aacaggatga cggtggcaaa ggcattctga acgcgttgta tgaagctaaa
    92401 aggcagcgtc cggaactgga tgtgcgggtg ctggtcgact ggcatcgtgc acaacgtgga
    92461 cgcattggcg ctgcggcatc taacactaac gctgactggt actgccgcat ggcgcaggaa
    92521 aatccgggcg tagatgttcc ggtttatggc attccaatca atacccgtga agcccttggt
    92581 gttctgcact ttaaaggctt tatcatcgac gatagcgtac tttatagcgg tgccagcctg
    92641 aacgatgttt acctgcatca gcacgataaa tatcgctacg accgttatca tcttatccgt
    92701 aaccgtaaga tgtcagacat tatgtttgaa tgggttacac agaatattat gaatggccgc
    92761 ggcgttaatc gtctggatga tgttaatcgg ccaaaaagcc cggaaatcaa gaacgatatt
    92821 cgtctgttcc gccaggagct gcgtgatgcc gcttatcatt tccagggcga tgccgacaac
    92881 gatcagcttt ccgtaacgcc gctagtgggg ctggggaaat cgagtctgtt gaacaagacc
    92941 attttccatc ttatgccttg tgccgagcag aaactaacca tctgtacgcc atacttcaac
    93001 ctgccagcaa tccttgtgcg caatattatc cagttgctgc gcgaagggaa aaaggtcgaa
    93061 attattgttg gtgataaaac cgcgaacgac ttctacattc cggaagatga acctttcaag
    93121 ataattggcg cattgcctta tctctatgag atcaatctgc gtcgtttcct gagccgtttg
    93181 cagtattacg tcaatactga ccagctagtg gttcggttat ggaaagatga cgacaacacc
    93241 tatcacctaa aagggatgtg ggttgatgat aagtggatgt tgatcaccgg taataacctg
    93301 aacccgcgcg cctggcgtct ggatctggaa aacgccattt tgatccacga tccgcaactt
    93361 gagctggcgc cacagcgaga gaaagaactg gagctgatcc gcgagcatac caccatcgtt
    93421 aagcactatc gcgatctgca aagtattgcc gattatccgg tgaaggttcg taaactcatc
    93481 cgccgtttgc gccgtatccg catcgaccga ttaattagcc gcatcctgta atcacaaccc
    93541 cgtcctgtac ggggtttgtt ttttggaggc cacgttttgc gtattctttt tgtctgttcg
    93601 ctattgttac tttctggatg cagccatatg gctaacgata gctggagcgg gcaggataaa
    93661 gctcaacact ttatcgcctc ggcgatgctt tccgccgccg gaaatgaata ttcacagcat
    93721 caggggatga gccgggatcg cagtgccatg tttggattga tgttctctgt cagtttgggg
    93781 gcgtcaaaag agctttggga tagccgcccc gaagggagcg gctggagctg gaaggatttg
    93841 gcctgggatg tcgccggtgc aagcaccggc tataccgtct ggcaactgac ccgtcactaa
    93901 agacgcatcc ccttcccttt gcgatgtagc atcaaagaaa ccagaaacgc caccacggcc
    93961 atcaaggtca cataccagaa gaaggctgtt tccattccta ttgatttcag cgacaacgct
    94021 acgtactccg ccgaaccacc aaatatagca ttagcgaccg catatgacag gccaacgcct
    94081 aatgcgcgaa cctgtgccgg gaacatctca gccttcagta ttccactgat tgatgtataa
    94141 aaactcacta tcagcagggc acacatcacc agaccaaaag cggcataagg cgaggaaacg
    94201 ttttgcaatg ctgagagaat aggaacggta aaaatggctg ccagcgaacc gaaacataac
    94261 attgaggtac ggcgaccaat cttatccgac agcgcgccaa tgagtggttg aataagcatg
    94321 aatacaaaca atgcggcagt cataatgcca ctcgccacgt tggcatgcat tcccgcagta
    94381 tttaccagat acttctgcat ataagtggtg aaggtataga aacaaaggga gcccgcagcg
    94441 gtaaaaccga gaaccatgat gaatgcacgg cgattgcgcc ataatccttt cagagatcca
    94501 gcttctttta aagcgcgcgt ttcttgttgc gaagtttcat ctaactgacg acgtaaccac
    94561 aacgccacaa cagctaacac agctcctaac gcgaaaggaa tacgccatcc ccactctctg
    94621 agtgcagcgt cttccatggt gtgttgtaaa accacgacaa ccagtagggc tagcagttgt
    94681 ccgccgatca acgtcacata ctgaaatgat gcgtaaaaac ctttgcgccc ttcaacggca
    94741 acttcactca tataggtggc gctggtgcca tattctccgc caacagataa tccctgaaat
    94801 aaacgcgcga gaagcaataa tgccggagcc cacgtaccta tagtttcata acctgggagg
    94861 caggcgataa ccagcgatcc gaaacacatc atacacaccg ataacagcat cgattttttg
    94921 cgaccatgtt tatcggctat gcggccaaat agccaaccgc ctattgggcg catcaggaat
    94981 cccgcagcaa aaacacctgc tgtttgtagt agttgagtcg tcgtgttccc ggaagggaag
    95041 aaaatgtggg caaagtagag tgaacagaac gagtagacat agaaatcgaa ccactcgacc
    95101 agattacctg aagaggcccc cacaatcgcc caaatgcggc gacgagtatc actacttgtc
    95161 agtttgctgt ctgccgttac agtactttca accatgccat tatgtctcct gccgtaatcc
    95221 gatgcttttg tcggtcgctt ttatttattt cttttggtaa agaaaatgtt atacatttgt
    95281 tgcgtatcat tatgcaacct taaccatgaa tttagttagc aggaaaatgg ttattgagga
    95341 gcttaaggat aaatttctgg taaggaggac acgtatggaa gtaggcaagt tggggaaacc
    95401 gcatccgttg ctgaatctgg catatgtggg agtataagac gcgcagcgtc gcatcaggca
    95461 tttttttctg cgccaacgca aaaaggccat cgtgactgat ggccttt
//
LOCUS       DAPHDX010000003        90958 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPHDX010000003 DAPHDX010000000
VERSION     DAPHDX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.003"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..630
                     /locus_tag="RH058_000199"
     CDS             46..630
                     /locus_tag="RH058_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3135846.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            710..1405
                     /locus_tag="RH058_000200"
     CDS             710..1405
                     /locus_tag="RH058_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3135847.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1435..3951
                     /locus_tag="RH058_000201"
     CDS             1435..3951
                     /locus_tag="RH058_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3135848.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDNNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3962..5053
                     /locus_tag="RH058_000202"
     CDS             3962..5053
                     /locus_tag="RH058_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3135849.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5096..5956)
                     /gene="rsmI"
                     /locus_tag="RH058_000203"
     CDS             complement(5096..5956)
                     /gene="rsmI"
                     /locus_tag="RH058_000203"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV3135850.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6021..8057
                     /gene="lpoA"
                     /locus_tag="RH058_000204"
     CDS             6021..8057
                     /gene="lpoA"
                     /locus_tag="RH058_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV3135851.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8015..8410
                     /locus_tag="RH058_000205"
     CDS             8015..8410
                     /locus_tag="RH058_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV3135852.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8430..9020
                     /gene="diaA"
                     /locus_tag="RH058_000206"
     CDS             8430..9020
                     /gene="diaA"
                     /locus_tag="RH058_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV3135853.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9030..9605
                     /gene="yraP"
                     /locus_tag="RH058_000207"
     CDS             9030..9605
                     /gene="yraP"
                     /locus_tag="RH058_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV3135854.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9719..10759)
                     /locus_tag="RH058_000208"
     CDS             complement(9719..10759)
                     /locus_tag="RH058_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV3135855.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10832..11467)
                     /locus_tag="RH058_000209"
     CDS             complement(10832..11467)
                     /locus_tag="RH058_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV3135856.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11595..12113
                     /gene="yhbO"
                     /locus_tag="RH058_000210"
     CDS             11595..12113
                     /gene="yhbO"
                     /locus_tag="RH058_000210"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV3135857.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12093..12536)
                     /locus_tag="RH058_000211"
     CDS             complement(12093..12536)
                     /locus_tag="RH058_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV3135858.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12587..12889
                     /gene="yhbQ"
                     /locus_tag="RH058_000212"
     CDS             12587..12889
                     /gene="yhbQ"
                     /locus_tag="RH058_000212"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV3135859.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12876..13379)
                     /locus_tag="RH058_000213"
     CDS             complement(12876..13379)
                     /locus_tag="RH058_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3135860.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13373..13897)
                     /locus_tag="RH058_000214"
     CDS             complement(13373..13897)
                     /locus_tag="RH058_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV3135861.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14106..15101
                     /locus_tag="RH058_000215"
     CDS             14106..15101
                     /locus_tag="RH058_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV3135862.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15110..15988
                     /locus_tag="RH058_000216"
     CDS             15110..15988
                     /locus_tag="RH058_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV3135863.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16194..17201
                     /locus_tag="RH058_000217"
     CDS             16194..17201
                     /locus_tag="RH058_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV3135864.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSRSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17319..18563)
                     /gene="mtr"
                     /locus_tag="RH058_000218"
     CDS             complement(17319..18563)
                     /gene="mtr"
                     /locus_tag="RH058_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV3135865.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18717..20606)
                     /gene="deaD"
                     /locus_tag="RH058_000219"
     CDS             complement(18717..20606)
                     /gene="deaD"
                     /locus_tag="RH058_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV3135866.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20599..20679)
                     /gene="yrbN"
                     /locus_tag="RH058_000220"
     CDS             complement(20599..20679)
                     /gene="yrbN"
                     /locus_tag="RH058_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV3135867.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20786..21670)
                     /gene="nlpI"
                     /locus_tag="RH058_000221"
     CDS             complement(20786..21670)
                     /gene="nlpI"
                     /locus_tag="RH058_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV3135868.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21779..23914)
                     /gene="pnp"
                     /locus_tag="RH058_000222"
     CDS             complement(21779..23914)
                     /gene="pnp"
                     /locus_tag="RH058_000222"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV3135869.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24161..24430)
                     /gene="rpsO"
                     /locus_tag="RH058_000223"
     CDS             complement(24161..24430)
                     /gene="rpsO"
                     /locus_tag="RH058_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV3135870.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24579..25523)
                     /gene="truB"
                     /locus_tag="RH058_000224"
     CDS             complement(24579..25523)
                     /gene="truB"
                     /locus_tag="RH058_000224"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV3135871.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25523..25924)
                     /gene="rbfA"
                     /locus_tag="RH058_000225"
     CDS             complement(25523..25924)
                     /gene="rbfA"
                     /locus_tag="RH058_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV3135872.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26088..28760)
                     /gene="infB"
                     /locus_tag="RH058_000226"
     CDS             complement(26088..28760)
                     /gene="infB"
                     /locus_tag="RH058_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV3135873.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28785..30272)
                     /gene="nusA"
                     /locus_tag="RH058_000227"
     CDS             complement(28785..30272)
                     /gene="nusA"
                     /locus_tag="RH058_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV3135874.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30300..30752)
                     /gene="rimP"
                     /locus_tag="RH058_000228"
     CDS             complement(30300..30752)
                     /gene="rimP"
                     /locus_tag="RH058_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV3135875.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30959..31035)
                     /locus_tag="RH058_000229"
     tRNA            complement(30959..31035)
                     /locus_tag="RH058_000229"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30999..31001),aa:Met,seq:cat)
     gene            31383..32726
                     /gene="argG"
                     /locus_tag="RH058_000230"
     CDS             31383..32726
                     /gene="argG"
                     /locus_tag="RH058_000230"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV3135876.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(32734..34359)
                     /locus_tag="RH058_000231"
     CDS             complement(32734..34359)
                     /locus_tag="RH058_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3135877.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34818..34904)
                     /locus_tag="RH058_000232"
     tRNA            complement(34818..34904)
                     /locus_tag="RH058_000232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34868..34870),aa:Leu,seq:gag)
     gene            complement(34919..35251)
                     /gene="secG"
                     /locus_tag="RH058_000233"
     CDS             complement(34919..35251)
                     /gene="secG"
                     /locus_tag="RH058_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV3135878.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35479..36816)
                     /gene="glmM"
                     /locus_tag="RH058_000234"
     CDS             complement(35479..36816)
                     /gene="glmM"
                     /locus_tag="RH058_000234"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV3135879.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36809..37657)
                     /gene="folP"
                     /locus_tag="RH058_000235"
     CDS             complement(36809..37657)
                     /gene="folP"
                     /locus_tag="RH058_000235"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV3135880.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37747..39681)
                     /gene="ftsH"
                     /locus_tag="RH058_000236"
     CDS             complement(37747..39681)
                     /gene="ftsH"
                     /locus_tag="RH058_000236"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV3135881.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39781..40410)
                     /gene="rlmE"
                     /locus_tag="RH058_000237"
     CDS             complement(39781..40410)
                     /gene="rlmE"
                     /locus_tag="RH058_000237"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV3135882.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40536..40829
                     /gene="yhbY"
                     /locus_tag="RH058_000238"
     CDS             40536..40829
                     /gene="yhbY"
                     /locus_tag="RH058_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV3135883.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40985..41461)
                     /gene="greA"
                     /locus_tag="RH058_000239"
     CDS             complement(40985..41461)
                     /gene="greA"
                     /locus_tag="RH058_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV3135884.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41709..43142
                     /gene="dacB"
                     /locus_tag="RH058_000240"
     CDS             41709..43142
                     /gene="dacB"
                     /locus_tag="RH058_000240"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV3135885.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43182..44354)
                     /gene="cgtA"
                     /locus_tag="RH058_000241"
     CDS             complement(43182..44354)
                     /gene="cgtA"
                     /locus_tag="RH058_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV3135886.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44370..45335)
                     /locus_tag="RH058_000242"
     CDS             complement(44370..45335)
                     /locus_tag="RH058_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3135887.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45462..45719)
                     /gene="rpmA"
                     /locus_tag="RH058_000243"
     CDS             complement(45462..45719)
                     /gene="rpmA"
                     /locus_tag="RH058_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV3135888.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45740..46051)
                     /gene="rplU"
                     /locus_tag="RH058_000244"
     CDS             complement(45740..46051)
                     /gene="rplU"
                     /locus_tag="RH058_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV3135889.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46310..47281
                     /gene="ispB"
                     /locus_tag="RH058_000245"
     CDS             46310..47281
                     /gene="ispB"
                     /locus_tag="RH058_000245"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV3135890.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47509..47787
                     /gene="sfsB"
                     /locus_tag="RH058_000246"
     CDS             47509..47787
                     /gene="sfsB"
                     /locus_tag="RH058_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV3135891.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47835..49094)
                     /gene="murA"
                     /locus_tag="RH058_000247"
     CDS             complement(47835..49094)
                     /gene="murA"
                     /locus_tag="RH058_000247"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV3135892.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49149..49403)
                     /gene="ibaG"
                     /locus_tag="RH058_000248"
     CDS             complement(49149..49403)
                     /gene="ibaG"
                     /locus_tag="RH058_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV3135893.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49563..49856)
                     /gene="mlaB"
                     /locus_tag="RH058_000249"
     CDS             complement(49563..49856)
                     /gene="mlaB"
                     /locus_tag="RH058_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV3135894.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49856..50491)
                     /gene="mlaC"
                     /locus_tag="RH058_000250"
     CDS             complement(49856..50491)
                     /gene="mlaC"
                     /locus_tag="RH058_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV3135895.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50510..51061)
                     /gene="mlaD"
                     /locus_tag="RH058_000251"
     CDS             complement(50510..51061)
                     /gene="mlaD"
                     /locus_tag="RH058_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV3135896.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51066..51848)
                     /gene="mlaE"
                     /locus_tag="RH058_000252"
     CDS             complement(51066..51848)
                     /gene="mlaE"
                     /locus_tag="RH058_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV3135897.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51856..52665)
                     /gene="mlaF"
                     /locus_tag="RH058_000253"
     CDS             complement(51856..52665)
                     /gene="mlaF"
                     /locus_tag="RH058_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV3135898.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIIAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52875..53852
                     /locus_tag="RH058_000254"
     CDS             52875..53852
                     /locus_tag="RH058_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV3135899.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53866..54852
                     /gene="kdsD"
                     /locus_tag="RH058_000255"
     CDS             53866..54852
                     /gene="kdsD"
                     /locus_tag="RH058_000255"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV3135900.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54873..55439
                     /gene="kdsC"
                     /locus_tag="RH058_000256"
     CDS             54873..55439
                     /gene="kdsC"
                     /locus_tag="RH058_000256"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV3135901.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55436..56011
                     /gene="lptC"
                     /locus_tag="RH058_000257"
     CDS             55436..56011
                     /gene="lptC"
                     /locus_tag="RH058_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV3135902.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55980..56537
                     /gene="lptA"
                     /locus_tag="RH058_000258"
     CDS             55980..56537
                     /gene="lptA"
                     /locus_tag="RH058_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV3135903.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56544..57269
                     /gene="lptB"
                     /locus_tag="RH058_000259"
     CDS             56544..57269
                     /gene="lptB"
                     /locus_tag="RH058_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV3135904.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57317..58750
                     /gene="rpoN"
                     /locus_tag="RH058_000260"
     CDS             57317..58750
                     /gene="rpoN"
                     /locus_tag="RH058_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV3135905.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58773..59060
                     /gene="hpf"
                     /locus_tag="RH058_000261"
     CDS             58773..59060
                     /gene="hpf"
                     /locus_tag="RH058_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV3135906.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59178..59669
                     /gene="ptsN"
                     /locus_tag="RH058_000262"
     CDS             59178..59669
                     /gene="ptsN"
                     /locus_tag="RH058_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV3135907.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59715..60569
                     /gene="rapZ"
                     /locus_tag="RH058_000263"
     CDS             59715..60569
                     /gene="rapZ"
                     /locus_tag="RH058_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV3135908.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60566..60838
                     /gene="npr"
                     /locus_tag="RH058_000264"
     CDS             60566..60838
                     /gene="npr"
                     /locus_tag="RH058_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV3135909.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61052..61684
                     /gene="yrbL"
                     /locus_tag="RH058_000265"
     CDS             61052..61684
                     /gene="yrbL"
                     /locus_tag="RH058_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV3135910.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61681..62409)
                     /gene="mtgA"
                     /locus_tag="RH058_000266"
     CDS             complement(61681..62409)
                     /gene="mtgA"
                     /locus_tag="RH058_000266"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV3135911.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62406..63059)
                     /gene="elbB"
                     /locus_tag="RH058_000267"
     CDS             complement(62406..63059)
                     /gene="elbB"
                     /locus_tag="RH058_000267"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV3135912.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63289..65625)
                     /gene="arcB"
                     /locus_tag="RH058_000268"
     CDS             complement(63289..65625)
                     /gene="arcB"
                     /locus_tag="RH058_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV3135913.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65721..66650)
                     /locus_tag="RH058_000269"
     CDS             complement(65721..66650)
                     /locus_tag="RH058_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV3135914.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67325..71785
                     /gene="gltB"
                     /locus_tag="RH058_000270"
     CDS             67325..71785
                     /gene="gltB"
                     /locus_tag="RH058_000270"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV3135915.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71798..73216
                     /gene="gltD"
                     /locus_tag="RH058_000271"
     CDS             71798..73216
                     /gene="gltD"
                     /locus_tag="RH058_000271"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV3135916.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73400..74527
                     /locus_tag="RH058_000272"
     CDS             73400..74527
                     /locus_tag="RH058_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV3135917.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74587..75051)
                     /locus_tag="RH058_000273"
     CDS             complement(74587..75051)
                     /locus_tag="RH058_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV3135918.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(75048..75923)
                     /gene="nanK"
                     /locus_tag="RH058_000274"
     CDS             complement(75048..75923)
                     /gene="nanK"
                     /locus_tag="RH058_000274"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV3135919.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75920..76609)
                     /locus_tag="RH058_000275"
     CDS             complement(75920..76609)
                     /locus_tag="RH058_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV3135920.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76657..78147)
                     /gene="nanT"
                     /locus_tag="RH058_000276"
     CDS             complement(76657..78147)
                     /gene="nanT"
                     /locus_tag="RH058_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV3135921.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78256..79149)
                     /gene="nanA"
                     /locus_tag="RH058_000277"
     CDS             complement(78256..79149)
                     /gene="nanA"
                     /locus_tag="RH058_000277"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV3135922.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGQIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79271..80062)
                     /gene="nanR"
                     /locus_tag="RH058_000278"
     CDS             complement(79271..80062)
                     /gene="nanR"
                     /locus_tag="RH058_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV3135923.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80442..81809
                     /gene="dcuC"
                     /locus_tag="RH058_000279"
     CDS             80442..81809
                     /gene="dcuC"
                     /locus_tag="RH058_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV3135924.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(81852..82349)
                     /gene="sspB"
                     /locus_tag="RH058_000280"
     CDS             complement(81852..82349)
                     /gene="sspB"
                     /locus_tag="RH058_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV3135925.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82355..82993)
                     /gene="sspA"
                     /locus_tag="RH058_000281"
     CDS             complement(82355..82993)
                     /gene="sspA"
                     /locus_tag="RH058_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV3135926.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83388..83780)
                     /gene="rpsI"
                     /locus_tag="RH058_000282"
     CDS             complement(83388..83780)
                     /gene="rpsI"
                     /locus_tag="RH058_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV3135927.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83796..84224)
                     /gene="rplM"
                     /locus_tag="RH058_000283"
     CDS             complement(83796..84224)
                     /gene="rplM"
                     /locus_tag="RH058_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV3135928.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84443..85570)
                     /gene="zapE"
                     /locus_tag="RH058_000284"
     CDS             complement(84443..85570)
                     /gene="zapE"
                     /locus_tag="RH058_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV3135929.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85764..86162
                     /locus_tag="RH058_000285"
     CDS             85764..86162
                     /locus_tag="RH058_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV3135930.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86316..87683
                     /gene="degQ"
                     /locus_tag="RH058_000286"
     CDS             86316..87683
                     /gene="degQ"
                     /locus_tag="RH058_000286"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV3135931.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87773..88840
                     /gene="degS"
                     /locus_tag="RH058_000287"
     CDS             87773..88840
                     /gene="degS"
                     /locus_tag="RH058_000287"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV3135932.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88903..89841)
                     /gene="mdh"
                     /locus_tag="RH058_000288"
     CDS             complement(88903..89841)
                     /gene="mdh"
                     /locus_tag="RH058_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV3135933.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVSKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            90276..90746
                     /gene="argR"
                     /locus_tag="RH058_000289"
     CDS             90276..90746
                     /gene="argR"
                     /locus_tag="RH058_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV3135934.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22434 a        24401 c        22556 g        21567 t
ORIGIN      
        1 cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa taaagttaca
       61 aaaacagcta ttgtgggctt acttgcgctt ttcgcaggta atgccgctgc aaccgatggt
      121 gaaatagttt ttgatggcga gattttaaaa tctgcttgtg aaatcaatga ctctgataag
      181 aaaattgaag ttgctcttgg tcactataat gccgaacagt ttcgttctgt tggcgatcgt
      241 agcccaaaaa ttccattcac tattccttta gttaactgcc cagtgactgg gtgggagcac
      301 gataacggca acgttgaagc gtctttccgt ttatggctgg aaacgcggga taatggcacc
      361 gtccctaact tccctaatct ggcaaaagtg ggctcttttg ctggcacagc ggcaacaggt
      421 gtgggtatcc gtattgacga tgcggaaagc ggaaacctta tgccactgaa tgctatgggg
      481 aatgataaca cggtttatca gatcccagcg gactccaatg gtattgtcaa tgttgacctc
      541 atcgcttact acgtatcaac cgttgaagca tcagaaatca ccccagggga agcagacgct
      601 gtcgttaacg taacgctgga ttaccgttaa tacgttacgg cgttatctga cctttcagat
      661 aacgcccttt tccttcctct ttctcgttgt atcaggttga aaaataacta tgtcaaaacg
      721 aacattcgcg gtgataataa ccttgttgtg tagcttctgt atcggccagg cgcttgcagg
      781 gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag aggctgcgtt
      841 acctgtcgca aacaaaggcg cagaaacgcc ttatttactg caatcatggg tagataatat
      901 agatggtaca agccgtgccc catttattat aaccccaccg ctatttcgtc ttgaagctgg
      961 cgatgactca tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc
     1021 gctgttctac attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc
     1081 taacgagttg acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct
     1141 gaagggacgg gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact
     1201 caatatatat aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac
     1261 cgatctcaca agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc
     1321 tgcatctggc ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg
     1381 gacagaaact cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca
     1441 caacgacacc accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgc
     1501 tgtttgccga tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag
     1561 tattatttcg accccattat gctggaaact acaaaaagtg gtatgcaaac aactgatctg
     1621 tcacgttttt caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg
     1681 aataaaaaga aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg
     1741 cagccacagt ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg
     1801 gcgctggctg aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt
     1861 acagctgctg aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca
     1921 ctgtaccgtg atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg
     1981 ctgtttacca actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc
     2041 caacgacagt acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgtaac
     2101 tattctacgt ggacacgcaa cgatcaggcg tcaagctgga acactatcag tagttattta
     2161 caacgtgata tcaaggcgtt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc
     2221 agtatttttt ccagctacac ctttactggc gtgcaactcg cttccgacga taatatgttg
     2281 ccaaacagcc agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc
     2341 gtgactatca ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt
     2401 gaaattaacg atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa
     2461 agtgacggta cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga
     2521 cctgggcatc taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat
     2581 agcaaggaac ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg
     2641 ctgtatggcg gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc
     2701 acacttggcg cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat
     2761 aaccggcact cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa
     2821 accaacacca atatcgctgt tagctactat cgctatacca acgatggcta ttttagtttt
     2881 gatgaagcca atacccgtaa ttgggactat aacagtcgcc aaaaaagtga aattcaattc
     2941 aacatcagcc agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac
     3001 tattggggca ataacgataa taacaggaat atctcagttg gggtttccgg tcagcaatgg
     3061 ggaattggtt acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc
     3121 gcactctctt tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc
     3181 tatcagatga ccagccagaa agatcgccca acccaacatg aaatgcgtct tgatggctca
     3241 ctgctggatg atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac
     3301 cataacagta gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac
     3361 agctacggta atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat
     3421 cctcatggcg tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat
     3481 ggggcttctg gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat
     3541 gcagtggttc cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag
     3601 ctgcccgata acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca
     3661 atggtagcgg cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat
     3721 cgcaacggta aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa
     3781 agtatcgtcg atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc
     3841 tggactgtac gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg
     3901 gattcagaac caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa
     3961 aatgaaaaga gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc
     4021 ctcctcagga gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt
     4081 tgatgatgtt acggtgaacc agacagataa cgtgacagga cgggagttta cctctgccac
     4141 gctaagtagc actaactggc aatacgcctg tacctgctct gcgggtaagg cagttaaact
     4201 tgtctatatg gtcagccctg tacttaccac cactggacat caagcaggat attacaaact
     4261 caatgatagt ctggatatta aaaccacatt acaggcgaat gacattccag gactcgtgac
     4321 tgaccagacc gtttctgtta acacccgatt cacacagata aaaagcaaca ccgtatattc
     4381 tgctgcaacc caaacgggtg tttgccaggg tgacacgtct cgttatggac ccgttaatat
     4441 tggtgcaaat accaccttta ccctgtatgt caccaagcca tttctcggct cgatgaccat
     4501 tccgaaaacg gatattgccg tcattaaagg cgcatgggtc gatggaatgg gaagcccgtc
     4561 tacaggtgac ttccatgatt tagtcaagtt atcgattcag ggaaatctca ccgccccaca
     4621 gtcgtgcaaa attaatcagg gcgatgttat taaggttaat tttggattca tcaatggtca
     4681 gaagtttacc acccgcaatg ccatgccaga cggttttact ccagtagact ttgatatcac
     4741 ttatgactgt ggtgatactt caaagattaa aaactcgttg caaatgcgca tcgacggtac
     4801 aactggggta gtagaccagt acaacctggt cgccagacga agaagttcag acaatgcgcc
     4861 cgatgtcggt attcgtattg aaaatctcgg cggcggagtt gcaaatattc cttttcagaa
     4921 cggtatcctt cccgttgatc cttccgggca tggcaccatc aacatgcgcg cctggccagt
     4981 taatctggtc ggtggtgagc tggaaacagg aaaatttcag ggcacagcca ccattaccgt
     5041 catcgtgcgg taaaagaaga cgttgcgttt tgattaacgc aacgcttcgg cactgttacc
     5101 cctgctgctc cagcgcatac ttatacagcg catttttctt cacgccgtga atttctgcgg
     5161 ccagcgccgc cgcttttttc agcggcagtt ctgcctgtag cagcgccagc gtgcgcaggg
     5221 catcggcggg taagtcttct tcctgtgctt tatgaccttc gacaatcagc accatttcgc
     5281 ctttgcgacg gttttcatct tcctttaccc acgccagcag ctcgccaacg ggcgcgccgt
     5341 gaatggtttc ccaggttttg gtcagctcac gcgccagaac cacgtagcgg gattcgccta
     5401 ataccgcaac gatatcttcc aggctatcta acagacggtg ggtagattca taaaaaatca
     5461 gcgtgcgcgg ctccgcttca atggctttta gcgcatcacg gcggcctttt gatttggcag
     5521 gtaaaaagcc ttcgtaacag aaacggtcag agggtaaacc cgctgcgctt aacgcagtga
     5581 tagcagcaca cggcccgggt agcggcacca cgcggatccc cgcttcacgg caggtacgca
     5641 ccagatggta gccaggatcg ttaattagcg gcgttccggc atcggaaacc agcgcaatgt
     5701 tttgcccctc ttgcagcttc gccagcagcg tttcagcttt ttgttgttcg ttatggtcgt
     5761 gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag caataaaccg gtgtgacgag
     5821 tatcctcggc ggcaatcaga tcaacggcct gtaatacctc taacgcacgc tgggtgatat
     5881 ccgccagatt gccgattggc gtcggtacaa tgtaaagctg gccctgagaa ttatccgccg
     5941 attggtgttg tttcattgtg tcgtccgtat tgccgattta atattgagca ttgcgtaaaa
     6001 aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa gccgcgcgtt
     6061 gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat actcccgatc
     6121 agtccactgc ttatatgcag ggtacggcgc aggctgattc tgccttttat cttcagcaga
     6181 tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt cgtgcactgg
     6241 tgaaagaagg taaaaccggg caggcggtgg agttgtttaa ccaactaccg caagaactga
     6301 acgatgctca gcgccgcgag aaaacactgc tggcggtaga gattaaactg gcgcagaaag
     6361 attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta gaacaaaacc
     6421 agcaagcccg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct tccattgatt
     6481 tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaag cagcagaata
     6541 ttgacgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat acgctggtga
     6601 tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc tggtttgata
     6661 accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcagaaa cgttatccga
     6721 acaacccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg tttaaaccag
     6781 cctcgaccaa caaaatcgcc ctgctgttgc cactgaatgg ccaggcagcg gtatttggtc
     6841 gcactattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca gtggcagctc
     6901 aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa accgtggatg
     6961 gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgaacag cctgcagccc
     7021 agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca cccgcaaatc
     7081 catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag atcttaagcc
     7141 aggttcagca ggatggcgcg agtattgtgg tcggtccgtt gctgaaaaat aacgttgaag
     7201 agttgctgaa gagcaacact ccgctgaacg tactggcact gaaccagccg gagaatatcg
     7261 aaaatcgcgt caatatttgt tacttcgcgc tttcaccgga agacgaagcg cgcgatgcag
     7321 cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca cgcagttcat
     7381 tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactaggc ggcggcaccg
     7441 ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac ggcggttctg
     7501 gtattgcttt aacgggtagc ccgattactc ccagagcgac aaccgactcc ggcatgacga
     7561 ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat aatggcggtc
     7621 gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc aaaccgatga
     7681 tcgccatgcg taacggtagc cagagcggtg caacgctgta cgccagctcc cgcagtgcgc
     7741 aagggaccgc tggcccggat ttccgactgg agatggaagg cttgcagtac agcgaaatcc
     7801 cgatgctggc aggcggtaat ctaccgttaa tgcagcaggc actcagcgcg gtgaataacg
     7861 attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg gcaaatcatt
     7921 tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga agcctgacgg
     7981 caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa caaggtcagg
     8041 tagtccccgc cagttaacca ccaaacagac cggcgatgcg tgggaagtac aagcgcgtcg
     8101 ctggctggaa ggcaaaggac tgcgatttgt cgccgctaac gtgaacgagc gtggcggcga
     8161 gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac gctatcgccg
     8221 ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac acaaattatt
     8281 acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg tggattgccg
     8341 gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg cctttaatga
     8401 ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct tgcttcactg
     8461 aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc tcccgtgcag
     8521 ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt ggtaatggaa
     8581 cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc gaaacggagc
     8641 ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg gcgattgcca
     8701 acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt catgcgggag
     8761 atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa gcagttgaag
     8821 ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc ggcgagcttg
     8881 caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt agtgctcgca
     8941 ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat aacacgcttt
     9001 tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc gcagtcctta
     9061 tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc gctgctgtgg
     9121 gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat ggtaccctgg
     9181 aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa acccgtatta
     9241 atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac gctgaacttt
     9301 cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg tataacgaga
     9361 ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg atcaccacca
     9421 aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg aaagtgacca
     9481 ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg aaagcggcgg
     9541 cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt acgtttatta
     9601 aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag gcatacaacg
     9661 atgcaaaccg tagatcggat aaggcgttta cgccgcatcc gacatgtttt ccctcaaatc
     9721 agaacaacag cgccagcccg ccgacaatca caccagacac tgccaccatc gcccctgtca
     9781 gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc aaactcactg
     9841 ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc agcatcatcg
     9901 tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct gccaccgcca
     9961 tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg aataaccaga
    10021 cgcgagcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta ctccagaaaa
    10081 gcgtccatag cgccctgccc cagcggctaa aaaaaccgcc ctgtgcttcc ggtatgtcaa
    10141 tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc agcgtcgcaa
    10201 tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc cagccgagga
    10261 caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc cagaatgcca
    10321 gcgcaccgcc catcgacacc tgttggcgac gcattcccgc cgcgaccgga gccgcacagc
    10381 aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg
    10441 attgcccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc aacgaaccga
    10501 ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc gcgtaatcca
    10561 acgccgcctg ccatgggtta gcatccgcct gcgcaaggat agatttaccg atactgtggg
    10621 tttcggcagc agtaaacgct ttgccgtagt aaggttccca tttcacatac cagagaccgg
    10681 caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt gccgcctgag
    10741 atgaagactg accagtcata gcattcccca ggaataagtt atgataattg agcgcgtgaa
    10801 tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt aattctgaag
    10861 agcttaaaat cgtcacgcct tcatgctcag gccgcatcga ctctgccagc atgacccgcg
    10921 ccacgtctct cgcatcaatg gatttccagt tacctggtaa caaacggaac agcggcgcaa
    10981 aaagcgtttc gttcatccgc tgtttgctac gatcgcccag taacatcgac gggcgagcaa
    11041 tggtcaattt cggccagttc tgggcgatta atgcttcttc catctcccct ttgacgcggt
    11101 tatagaaaaa cggcgagtgg gcattggcac ccatcgcact gactaccaac atatgctgcg
    11161 cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg gcatgaataa
    11221 acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac acaatgtcga
    11281 tgggatcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga ttaaacaccc
    11341 cgggcatatc gcccaacgga cgtcgcgtcg gcgcagcaat ggcgttaact ttcggttcgt
    11401 taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta atcagtacct
    11461 gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc atgcaccagg
    11521 cttaatagac catgaggtaa ttatccccga ttgtgggaaa ttcgcctcat ccaatgcaac
    11581 aacgcggagg aagcatgagt aagaaaattg ccgttttaat cactgatgaa tttgaggatt
    11641 cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg attaccattg
    11701 aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg accatcgata
    11761 aatccatcga tgaagtgacg cctgcggagt ttgatgccct gctgctaccg ggcggccatt
    11821 caccggatta tctgcgtggg gacaaccgtt ttgtcacctt tacccgtgat tttgtgaata
    11881 gtggcaaacc ggtgtttgcc atctgtcacg gcccgcagtt gctgatcagc gccgatgtga
    11941 ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa aatgcgggcg
    12001 cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc agccggacac
    12061 cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc tgagtcgcgt
    12121 agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat ttcatcgagc
    12181 cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg attgtacgcc
    12241 ttgcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt aaactgcaca
    12301 ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac tgccgcctgc
    12361 ggcccgctca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa ggcgactttc
    12421 tgcgcatcaa aaagataaaa ggcattagcg caccagagtt ccccttcctg ctgcacacac
    12481 caggtgacaa catgttgttt tgccagccaa cggctgatgg cgatgagtgt ttccattgct
    12541 gttctctttt atactgtggg cctgacctta acatgcgatg cagacgatga caccctggtt
    12601 tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca cggatgtcga
    12661 acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg ggaaaggaga
    12721 actgacgctg gcgttttctg ctccagtggg cgatcgttcg ctggcgttac gggcggaata
    12781 tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag gcgcagggtt
    12841 tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa atgctcgtga
    12901 tactcaacca ggccggtaac gccattcagc gcgtcatccg ctaaacgatg tacctggaag
    12961 gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag ttcaaagccg
    13021 aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc gaactcatta
    13081 agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg gtatttttca
    13141 tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac atcaaccgga
    13201 ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag ccccagcgtc
    13261 agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact ttcgaatgag
    13321 cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg aattagcatg
    13381 gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat
    13441 ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt
    13501 ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc agccgacgct
    13561 ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga tcgctggcgt
    13621 cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg ccattcacca
    13681 ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag cggccttcaa
    13741 gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag acctgctcaa
    13801 gaacctgccg ttttagcgca aatggcgtca gttttaccgg tacactcaac agagatggcc
    13861 ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc tgtttcaaca
    13921 atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac aattccatat
    13981 gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt gtcgcagcaa
    14041 ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gatagcgcaa ccttcaggaa
    14101 aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg gccatcgaaa
    14161 acggcgcaga tgctgtttat atcgggctaa aagatgatac caatgcccgt cacttcgccg
    14221 gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat caacatcgcc
    14281 gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc cgttggcagc
    14341 gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca
    14401 tgctggaata cgccgccgag cgttatccgc atattgagcg ccacgtatcg gtgcaggctt
    14461 cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgacgtt gcccgcgtgg
    14521 tgctgccgcg cgtgttgtcg attcatcagg tgaaacaact ggcacgggtc acacctgtac
    14581 cactggaagt ctttgctttc ggcagcctgt gcattatgtc ggaaggtcgt tgctatctgt
    14641 cgtcgtatct gacgggtgag tcgcccaaca ccgtaggcgc gtgttctccg gcccgtttcg
    14701 tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg ctgatcgacc
    14761 gttatcagga cggcgaaaac gcaggttatc cgacgctatg taaagggcgt tatctggtgg
    14821 acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg gaactgctgc
    14881 cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag cgtagcccgg
    14941 cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt aaggccgatc
    15001 cgcaaaactt tgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg tccgaaggca
    15061 cgcaaaccac tcttggcgcg tatcaccgta aatggcagtg agaagagcaa tgaaatattc
    15121 cttagggcca gtgctgtggt actggccaaa agagacgctg gaagattttt atcagcaggc
    15181 cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca agcgccgggc
    15241 aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagtg gcaagcagat
    15301 tgtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac tgaaacgcta
    15361 tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc
    15421 tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg cggtgacgct
    15481 gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga
    15541 ctggctggtg aatctgctta atcagtgcga tgagctgggc attcgcaacc agtttgaagt
    15601 ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct ttaccgcgcg
    15661 ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc cgaacgggcg
    15721 caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc agaccatgag
    15781 cggctacgtt tacaacctcg gtaacgagct ggcatccatg caggggctgg ttgatgtggt
    15841 ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc gcgctaatga
    15901 aaatggcgct gcgccactac cgttgacggc aaacagcgac tgtaacggct actggcggcg
    15961 gctggcagga ctggagctgc aagcctaagt aaatatgctc gtcatacttc aagttgcatg
    16021 tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg gggattcact
    16081 ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg ttaacaactt
    16141 taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg
    16201 ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt tcttcagcgc
    16261 gggaagcatt ctcccgctct ctcgacctcg cccgtctggc tgaaaagcgc ggctatcatc
    16321 gctactggct ggcggaacac cacaatatga ccggcattgc cagtgctgca acatcggtat
    16381 tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt
    16441 tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat acgctctatc
    16501 cggggcgaat cgatttgggg ctgggtcgtg ctccgggtag cgatcagcga accatgatgg
    16561 cgctacgtcg tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg
    16621 tggactggtt tgacgcccgc gatcccaatc cgaatgtgcg cccggtacca ggctatggcg
    16681 aaaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg gcggcgcagc
    16741 ttggtctgcc gtttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc
    16801 atctttatcg cagcaacttc aaaccgtcgg ctcgactgga aaaaccatac gcgatggtgt
    16861 gcatcaatat tatcgccgcc gatagcaacc gcgacgctga gtttctgttt acctcaatgc
    16921 agcaagcctt tgtgaagttg cgccgtggcg aaaccgggca actgccgcca ccgattcaaa
    16981 atatggatca gttctggtcg ccgtccgaac agtatggtgt gcagcaggcg ctgagtatgt
    17041 cgttggtagg tgataaagcg aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg
    17101 acgccgatga gattatggtc aacgggcaga ttttcgacca ccaggcgcgg ctgcattcgt
    17161 ttgagctggc gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag
    17221 ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg catcaggcat
    17281 tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa
    17341 ttaaagctcg ataaaatatg gaccagtgcg ttgccaacgc caaacaccag aatcagcgta
    17401 atcatcggct tgccgcccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg
    17461 cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc agcataacca
    17521 atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg ggcaaaggtc
    17581 agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag atctgccaga
    17641 tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac
    17701 acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ctgtaccagc
    17761 acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg gatgttaccc
    17821 atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg
    17881 catttcacga tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg
    17941 tgataaccaa acgacgccag gcagaacggc aatgtcatca gcagatacgg cgcataagat
    18001 gcattgcttt ctgcaacgtt gaacaatgtc gcaggctgca catgccccaa caggctgcca
    18061 aaggtgagga agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg
    18121 gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc
    18181 cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga accactggca
    18241 gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc gttgaccacg
    18301 ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa
    18361 ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc
    18421 agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc
    18481 cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt
    18541 tgggtggtgg ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt
    18601 tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa gccccgatgg
    18661 taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac
    18721 gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc gcggccttca
    18781 cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg ttcaccactg
    18841 aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc acgacgctca
    18901 ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg
    18961 cgagtacgcg taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg
    19021 gagtgagaag caaacagctt gatgttacca atgtaacggc tgctgatgtc gccttcgtta
    19081 gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg
    19141 tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcacca
    19201 cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt
    19261 ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg tgccattttc
    19321 agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt
    19381 ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta
    19441 gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc
    19501 ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca
    19561 acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg
    19621 taagactcag aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc
    19681 aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc tttcaggcgt
    19741 tccagtgtct gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc actgttgtag
    19801 ccgttacgct caagagcttc agccacttcc agagtcgcat ttttggtacg aacgaagata
    19861 atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata
    19921 ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaatg
    19981 cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg
    20041 gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg
    20101 tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt
    20161 ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca
    20221 acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac
    20281 agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc
    20341 tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga
    20401 tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc
    20461 atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat
    20521 ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct
    20581 gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag
    20641 tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca
    20701 aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa
    20761 gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt
    20821 cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt
    20881 tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt
    20941 agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatccg
    21001 ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact
    21061 cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt
    21121 ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag
    21181 gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac
    21241 cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct
    21301 caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata
    21361 tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat
    21421 cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa
    21481 gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca
    21541 gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc
    21601 aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa
    21661 aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct
    21721 aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt
    21781 actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg
    21841 cttctttaat gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta
    21901 cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag
    21961 agatgtgtac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga
    22021 tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga
    22081 tacgacgaat agcgtgtttc gctttctcgc cgtcggtcgc tgcgatcttc acagtaccgt
    22141 catcttcgat ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagaaccgc
    22201 ctttaccgat aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg
    22261 cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat
    22321 gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt
    22381 caattttgat atccatctgc aatgcagaga taccgtcgcg ggaacccgca actttgaagt
    22441 ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc
    22501 cttctttcac cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt
    22561 ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag
    22621 tgatttcaga cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca
    22681 gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca
    22741 tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag gtgtcggtac
    22801 gttcgcccat cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa
    22861 ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc
    22921 cagtacgcac atccagacca cgaatcatat ctttttcacg accgtcgata cgcggttcgc
    22981 ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt tcacccagtt
    23041 cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga
    23101 catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca ctcagacgag
    23161 cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt
    23221 cccaacgtgg tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct
    23281 gttgttcatg accgaacact actgcgccca gcatctggtc ttcgctcagc aattcagctt
    23341 cagattcaac catcagtaca gcggcttcag taccagcaac aaccagatcc agtttgctct
    23401 ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcggg
    23461 cagcaccaat cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa
    23521 tcatcgcgac gatatccgga ttaacttgcg ggttaacaga aaccacggtg gcgataacct
    23581 gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg
    23641 cgatcagggt ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac
    23701 gaccagcagc gtaagtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg
    23761 gtttggcttt tttctggcca acaacggtaa cgaataccgc ggtgtcatcc atgctaacca
    23821 taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc
    23881 cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac
    23941 agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca
    24001 accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg
    24061 aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa
    24121 aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc
    24181 gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac
    24241 gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg
    24301 caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcggtag
    24361 aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac
    24421 ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag
    24481 cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat
    24541 tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc
    24601 aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg
    24661 ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg
    24721 ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa
    24781 gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc
    24841 tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga
    24901 tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca
    24961 cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc
    25021 agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca
    25081 cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt
    25141 gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc
    25201 agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa
    25261 gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga
    25321 tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg
    25381 tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc
    25441 gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga
    25501 ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt
    25561 cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag
    25621 agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca
    25681 ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt
    25741 catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt
    25801 cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga
    25861 tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg
    25921 ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag
    25981 gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcgcatca ggcagtccat
    26041 gcaggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta
    26101 cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag
    26161 ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag
    26221 cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc
    26281 gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc
    26341 ggtgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga
    26401 gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag
    26461 taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg
    26521 ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata
    26581 ccccctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag
    26641 gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat attcacttcg
    26701 tgaacttcgc cttcggtcat gttggcaaac atgttctcga gtttagattt ttgctgacgc
    26761 gccagtttaa cttcgcggaa tttaccctga cgatagagtg caacttcacg cgctttcttc
    26821 tcgtcacgta caacggtaac ttcatcaccc gcagccggaa cgccggacag gccgaggatt
    26881 tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca
    26941 cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca
    27001 cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca
    27061 ccgctcgcca tacctttacg taccgctttt agctccagaa cttccgcctg cagcaggata
    27121 gcgtccagca gttcatcgat accggtaccc gctttcgcag atacgtgtac gaactggctt
    27181 tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt aacacgatcc
    27241 ggatcggctt ctggtttatc gatcttgttc actgcaacca ccaccggtac ctgcgccgct
    27301 ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc agcaacaacc
    27361 aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa cgcggcgtgc
    27421 cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg
    27481 tgctgggtaa tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc
    27541 agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca
    27601 gccgcagccc ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc
    27661 aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc
    27721 tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta
    27781 accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac aacgtcacgg
    27841 ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt acgttttccg
    27901 cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt
    27961 ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg
    28021 acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagttgt gacatgataa
    28081 tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga
    28141 cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca
    28201 gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt
    28261 ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct
    28321 tgttcagcag cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc
    28381 tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc acgctgcgct
    28441 tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc
    28501 ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat gttaagggtg
    28561 ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg attcaggtgg
    28621 tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt ccggatacct
    28681 gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc ggccagcgtt
    28741 ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc ttcgtcaccg
    28801 aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc ggtcaaccct
    28861 tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt acaaacgcca
    28921 cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag cagatcgtca
    28981 gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc cagtgcattt
    29041 ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc
    29101 tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc taccagaaca
    29161 gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct
    29221 tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc ccaaccgctc
    29281 agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc caggttaccg
    29341 gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga agcaacgtct
    29401 gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag gacgatatcg
    29461 atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg catacctaca
    29521 caggcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt cgcacgagaa
    29581 cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc tggcacttca
    29641 atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag ttgcgcgcca
    29701 cgcgcttccg ggcgaacgga atagagcacg ccacgaacgc ggtcgccagg gcggaagttt
    29761 tcacgcggca gcatatcttc gcgcaggatc acggcttcag cattgttgcc cagatccaga
    29821 gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc ttcgtgttca
    29881 cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac gataacctgt
    29941 tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc ttcaacgtaa
    30001 tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat ttccttggtc
    30061 ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc accgcttttg
    30121 cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt tgctgtcgcc
    30181 agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc attggatacg
    30241 gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca tccagactat
    30301 taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac ttcatcttta
    30361 ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc ctgccattta
    30421 cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac aaaacgggcg
    30481 tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc caggttataa
    30541 gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct cacatcagca
    30601 caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt ggatgtgcga
    30661 ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg cgcagtaatc
    30721 atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata aaaaaagggc
    30781 ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg gggctttata
    30841 taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga ttttttcaaa
    30901 tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta agggaaagtg
    30961 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    31021 ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa
    31081 tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc
    31141 acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag ttcactttca
    31201 tttgttgaat acttttgcct tctcctgctc ccccttaagc gcattatttt acaaaaaaca
    31261 cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt tattttgcat
    31321 aaaaattcag tgagagcgga aatccaggct catcatcagt taattaagca gggtgttatt
    31381 ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc gctttttccg
    31441 gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagcg gttccttatg
    31501 catatactgc aaacctgggc cagccagacg aagaggatta tgatgcgatc cctcgtcgtg
    31561 ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg
    31621 aaggtattgc cgctattcag tgtggcgcat ttcataacac caccggcggc ctgacctatt
    31681 tcaacacgac gccgctgggc cgcgccgtga ctggtaccat gctggttgct gcgatgaaag
    31741 aagatggcgt gaatatctgg ggtgacggta gcacctataa aggaaacgat atcgaacgtt
    31801 tctatcgtta tggtctgctg accaatgctg aactgcagat ttacaaaccg tggcttgata
    31861 ctgactttat tgatgaactg ggcggccgtc atgagatgtc tgaatttatg attgcctgcg
    31921 gtttcgacta caaaatgtct gtcgaaaaag cctactccac agactccaac atgcttggtg
    31981 caacgcacga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc gtcaacccaa
    32041 ttatgggcgt gaaattctgg gatgagagcg tgaagatccc ggcagaagaa gtcacagtac
    32101 gctttgagca aggtcatccg gtagcgctga acggtaaaac ctttagcgac gacgtagaaa
    32161 tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc gaccagattg
    32221 aaaaccgtat catcgaagcg aaaagccgtg gtatttacga ggctccgggg atggcactgc
    32281 tacatatcgc ttatgaacgt ctgttgaccg gtattcacaa cgaagacacc attgagcagt
    32341 atcatgcaca tggtcgtcag ttgggccgtc tgctgtacca ggggcgttgg tttgactccc
    32401 aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc actggtgaag
    32461 ttaccctgga gctgcgccgt gggaacgatt attcaatcct gaataccgtc tcagagaacc
    32521 tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg
    32581 atcgtattgg tcaattgacc atgcgtaatc tggatatcac tgatacccga gagaaacttt
    32641 ttggttatgc caaaactggc ctgctctcct cctctgccac ttcaggcgtg ccgcaggtgg
    32701 agaatatgga aaacaaaggc cagtaattcg acatcaaccc tgcccttcag gggtggggtt
    32761 accttgctta tcctctgtaa actcctttct gagcatcaca taatcgaaaa catcatggtt
    32821 tgcatccaca acccgtgagt ctcctttata gtgtgcaatc acttcctcaa gcgtttgcgg
    32881 ctgttccggc tttgttaccc ccatccacgc attaataagg taattattgt aagctgtcga
    32941 aaagatgttg ttttccgttg tgcgatccac gccatcccca agaacggggc tataccagat
    33001 aaacatcggg acatgatatg cctgctggct agcctccctg cctccatgaa aatagacgtt
    33061 cttcttcgta gggtcacgtt ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg
    33121 atcttttaat aattcaaaaa cctgacctag caaactatct gtgtaatgaa tggagttgtc
    33181 atagcaggca tcctgatcgt cctgcggttg aaacacggcg ctggattgcg gataggcgct
    33241 acaagccggt tcatggcttc catttaaatg aagaacaatc agtttctttt gctgcgtatt
    33301 ttgctgtaat gcttgcgata aatgcggcaa caacaattca tcaaatcctc tgacataaac
    33361 tgtttccatg gcgcgcatgg cgatactggt aactgctgta ccattctgcc gaaaagcgga
    33421 ttgcgagctt agccagaaag tctgaaatcc tgcttgatta gccatattaa taatgttgtc
    33481 ggggtaatta tgaatgtcat gactcaaaac agaatcagca gttaaagaaa ggggaaccga
    33541 cagcgcagtg taaggtgcgc cgcttattgc ttgattaaac agtttgatct gttttctttg
    33601 tgcttcaact tgcggtgtcg tagagcgtgt atatccatac aaagacatat tgtcgacacg
    33661 tacagactcc cccacaatca acacgtaggt atcaatacct gtatccctga ctgataattg
    33721 aaaatacggc accgtgtttg caattgagag taatctttga tgctctttcg ctgctaaagc
    33781 aaaatagttg agattgaaaa acggcgtata ggtagcaaat cgcgacgcta gtatatatgg
    33841 actgaacgct tttttatttt ttgcatcttt ataagcaaat tgacaagcgg aaaataaact
    33901 gcccgaaatg acaatcagca ataatattcc tgtcactttt tttgtcggca aggaaacatc
    33961 atattttatt attactgcca aaaaaagaag ggataaaaaa gcaaaggcac atagataagg
    34021 gatatacatc cccagcattt tgacaacttc atccggatca ctctggagca cactaatcgc
    34081 aaatccatca ttgaaagttg taccaaaggt acaccatgaa tagaggctaa tactcatgtc
    34141 tgccgcagtc agtaacacaa atggtgcggc aacaagcagg cgaagcggaa gggatgatat
    34201 acgttttaca accaggatgg tcaggacgaa gaacagcaat cgctgaacca tatgcgcgcc
    34261 ggaagaggcg actaagttcg taataccaaa aacaataaca caaagataca acaaccaatg
    34321 agatttaaaa cttctagcaa atttgttgaa tactgtcatc cagaaatcca agttcacaac
    34381 caatataaac aattatcaac aatgttcaat tttatatcag acatgttttt caactctgat
    34441 ccatatcaga ccgaaacact gttagttttt tctttttaaa tgatgcggcc attgcgttct
    34501 cagtaattaa ataacattat caatgcgtct ctaaacatta cgcagaacgt agccatcagt
    34561 agttatactg acaggagcag gatggtttat cgagaagtaa acatatccgt attcgtaccg
    34621 gaactggagt tgacacaata aagtgccaat tatgtcagca gaagggaaaa gtttataact
    34681 aaggcgtacc ggcaccatcg tttcaaggta ccagctacga gtaaagcaac tggacgagat
    34741 acagatacct gacaaccatt cctcagacca ggaccaaaac gaaaaaagac gcttttcagc
    34801 gtctcttttc tggaatttgg taccgaggac gggacttgaa cccgtaagcc ctattgggca
    34861 ctaccacctc aaggtagcgt gtctaccaat tccaccacct cggcacggat actacttttt
    34921 agttcgggat atcgctggtc ggcttagccg gagcagctgg ttgagtttgt tcggttttca
    34981 ccggtgcact cagattttcc cattcgctac ctttattggt tttgttgcta ttgatgttac
    35041 ccagcaccag actgatgatg aagaataacg ttgccagcag cgccgtcatg cgggtcatga
    35101 agttaccaga accacttgaa ccaaacagcg tagcggaagc gcctgctccg aaggaggctc
    35161 ccatatcagc gcctttacct tgctgcagca tgatcagacc aacaaggcca attgccacaa
    35221 taaggaaaac tactaaaaga gcttcgtaca taatcaacct gttccttgcg gagttgccgc
    35281 gtaccaatgc ttcaaccaat aaagcgggag tttttaatct ttcccactga agcgggtgtg
    35341 aatactaacc aaagcgaatg accttcgcaa gggcaatttt atcgcattgt atcaactgcg
    35401 gaaaaaaaca gcaaaaccga tgtgttcgct gagaaaaaag gcgacaatcg ccgccttttt
    35461 agccagttat ctaacgcttt aaacggcttt tactgcatcg gcgatgcggt gtgcaaattc
    35521 agtcacctgc gcttcgtctt cgccttccac catcacgcga attaacggtt cggtgccgga
    35581 tttacgcagc aacacgcgtc cacggttgcc cagcgcagct tcaacctctg cggtcacggc
    35641 tttaactgac tcatgctcaa gtggatcgcc gctacctgcg gtgtaacgta cgttaaccag
    35701 aatctgcggg aacattttca tgccgctgca aaggtcgtgc aggctcatat ggttacgtgc
    35761 catcgccgcc agcacctgca agccagcaac gatgccgtca ccggtagtag ttttatccag
    35821 taggatcaca tgaccggaat tctctgcacc gatacgccag cctttctcct gcattttttc
    35881 cagtacgtag cggtcaccca ctttcgcgcg cgcaaatgga attcctaact gtttcagcgc
    35941 cagttcaagc cccatgttgc tcatcaatgt acccacagcg ccaccacgca gctggccctg
    36001 acgaagacct tcacgcgcga tgatatacat gatctgatcg ccatcgactt tattgccttc
    36061 atggtcgacc ataatcacgc gatcgccatc gccgtcgaag gcaataccga gatccgcttt
    36121 ttcagccagc acacgagcct ggagcgcgcg aacgtcggta gcccccactt cggcattgat
    36181 gtttacaccg tttggctcac aaccgatagc gataacgttc gcccccagtt cgcgcagcac
    36241 gttcggcgcg atgtgataag tcgcaccgtt tgcacaatcc accacaatct tcagttcact
    36301 gaggctaagt tcgttcggga acgtggcttt gcaaaactcg atatagcgac ccgcggcatc
    36361 aacgatacgg ctggctttac ccagttctgc cgaatcaacg cagctgatct ccttttccat
    36421 ttccgcttcg atggcctctt ctaccgcatc cggcagtttg gtgccgtcga tagagaagaa
    36481 tttaatccca ttatcgtaga acgggttatg cgatgcagat atcacaattc cggcctctgc
    36541 gcggaaggta cgcgtcagat aagccacggc cggtgttggc atcgggccag tgaagagtgc
    36601 ggaaaggccc gctgccgcca gacccgcttc cagtgctgac tccagcatat agccagaaat
    36661 acgcgtgtct ttaccaataa taatcttacg ggagccgtgg cgcgccagca ctttacccgc
    36721 ggcccaaccc agcttaagca caaaatcagg tgtgatcggc gcatccccta cacgaccacg
    36781 aatcccatcg gtaccgaaat atttacgatt actcatagcg tttgttttcc tttgcagaca
    36841 gagtggcttc caccacccgc atcgcttcta cggtttcttt gacgtcatga acacgaatga
    36901 tgtgcgcgcc ttgcattgcg gcaatgaccg cacaggccag actaccgctc aggcgctcgg
    36961 acggccccac gttcagcagc tgcccaatca tcgattttcg tgacataccc accaacagcg
    37021 gcaggttgaa atggtgaaat tcagccaggc gcgccagtaa tgaatagtta tgggagagat
    37081 ttttaccgaa accgaatccg gggtcgagca acaatttctc ttttgcgata cccgcctgct
    37141 cgcaacgtgc tatttgctca ataaagtagc gattcacttc tgcaaagaca tcgtcatact
    37201 tcggagcttc ctgcatggtt tttggatttc cctgcatatg catcagacaa accggtaaac
    37261 cggtttctgc agccgcctcc agagcgccag gttcggaaag ggagcggata tcattaataa
    37321 tgtgagcgcc aactttcgct gactcacgga tgacttctgg tttggatgta tcgaccgaga
    37381 tccagacttc gaagcgttga gcaattgcct caaccacagg aataacacgt tgcaactctt
    37441 cttcaacgct aacttccgcc gcccctgggc gcgtggactc gccaccaacg tcaatgatcg
    37501 tcgcgccagc attgatcatc agattcgcat gtttcaccgc atctatcagc gagttatgcg
    37561 tgccaccatc cgaaaaggaa tcaggcgtga cgttgaggat ccccattacg tgaggatggc
    37621 taaggtccag tgaagtaccc tgggcaaaga gtttcatgat gttatccctg gtatgaattg
    37681 ataagaaaaa accccggagc acgccccggg gttttcggta caaatacagt catctgatgc
    37741 gggaacttac ttgtcgccta actgctctga catggtgtta cccgggttcg gcgtacgcgg
    37801 ttcatcaacc ggacgaggag cctttggact accattgtcg ccagaattgt tagaagcgcc
    37861 tggttcttcc cagcccgctg gcggacgtac atcgcgacgt gccatcaggt catcaatctg
    37921 cggtgcgtcg atagtctcat atttcatgag agcatctttc atcgcatgca gaatatccat
    37981 attgtcggtc agaagctgac gcgcacgatt atagttacgc tcaatcagtg ctttcacttc
    38041 ctggtcgatg atacgtgcag tttcatcgga catatgtttc gctttcgcta cgctacggcc
    38101 gaggaatact tcaccttctt cttccgcgta cagcagtgga cccaatttct cagagaagcc
    38161 ccactgggtc accatgttac gtgccagatt ggtcgcaact ttaatatcgt tggacgcacc
    38221 ggtagaaaca tgttccggcc catagatgat ctcttctgcc agacgaccac cgtacagcgt
    38281 agaaatctgg ctttccagtt tctgacggct ggcgctgatt gcgtcgccct caggcaagaa
    38341 gaaagtcaca cccagcgcac gaccgcgtgg gataatcgtc actttgtgca ccggatcgtg
    38401 ttccggcacc aggcgaccga taatcgcatg acccgcttcg tggtaagccg ttgattcttt
    38461 ctgcgcttcc gtcatcacca tggagcgacg ttccgcaccc atcatgattt tgtctttcgc
    38521 tttctcgaac tcaaccatcg acacaacgcg tttgttgcca cgagcagcga acagtgccgc
    38581 ttcgttcacc aggttcgcca ggtcagcacc ggagaaacca ggagtaccac gggcaatgat
    38641 tgccgcgtcg atatcggggg ccaatggtac gcgacgcatg tgaactttca ggatctgctc
    38701 acgaccgcga acatctggca agccgaccac aacctgacgg tcgaaacggc caggacgcag
    38761 cagggccggg tcgagaacgt ccggacggtt agtcgcggcg ataacgatga taccttcgtt
    38821 accttcgaag ccatccatct caaccagcat ctggttcaga gtctgttcac gttcatcgtg
    38881 accaccgccc agaccagcgc cacgctggcg gcctacggcg tcgatttcat cgataaagat
    38941 gatgcacggt gccgctttct tcgcctgttc gaacatgtca cgaacacggg atgcacccac
    39001 accgacgaac atttctacga agtcagaacc ggagatagta aagaacggaa ctttcgcttc
    39061 gcctgcaatc gctttcgcca gcaacgtttt accggtaccc ggaggaccga ccatcaggac
    39121 gcctttcggg atcttaccgc cgagtttctg gaagcggctc ggctcgcgca gatactctac
    39181 cagttcagca acttcttctt ttgcttcgtc gcagcccgca acgtcagcaa aggtcgtttt
    39241 gatctgatct tccgtcagca tgcgcgcttt gctcttacca aacgacatgg cacctttgcc
    39301 accgccgccc tgcatttgac gcatgaagaa gatccagaca ccaattagca acagcatcgg
    39361 gaaccaggag atgaagatag aagccagcag gcttggttct tcaggcggtt caccgacaac
    39421 cttgacgttc ttggtcaaca ggttatccag taatttcgga tcctgaaccg gaatgtaagt
    39481 ggtataacgg ttactatctt tcttggtaac gttgatttca cgtccgttga tacgcgcttc
    39541 acgaacctgg tcgttattga cctcttgtag gaaggtagag taatccacct tacggccatt
    39601 agactcgctg ggcccaaagc tctggaatac tgacatcagc acaacggcaa tgaccagcca
    39661 gagtattagg tttttcgcca tgtcactcaa gggattaacc tcttattaca actgtgttaa
    39721 aaacagcgtc aggatactct atatccagca tctttcaaac tttcgtctga aatctcccgg
    39781 ttagggttta cgcccggtcg ctacaatata cacttcccgc gaacgtgcac gagaagagtc
    39841 cggcttacga actttgacct tcgtaaacag ggagcgaatt tcccttagat actcatcgaa
    39901 accttcgccc tggaacacct tcactacaaa actgccacct ggcgctaata catcacgaca
    39961 catttctaac gccagttcca ccagatacat ggcacggggg atatccaccg ccggtgttcc
    40021 gctcatgttt ggtgccatat cggacatgac aacctggact ttgctgtcgc caacgcgctc
    40081 cagcagtgct ttcatcacca gttcatcacg aaaatcgccc tgaagaaagt ccacaccaac
    40141 gataggatcc ataggtaaaa gatcgcaagc gatgatgcgg cctttgccgc caatttgggt
    40201 gaccacatat tgtgaccaac cacccggagc agcaccaagg tcgacaaccg tcattcccgg
    40261 tttaaagagt ttgtcacttt gctgtatttc atcaagttta aaccaggcac gggaacgtaa
    40321 cccctttttc tgtgcctgtt gaacatattt atcgctaaag tgttcctgaa gccagcggct
    40381 ggagctggca gaacgcttct tacctgtcat ttaactttcc catggggata actcatcgta
    40441 accaattgcg taaattttta cgcgcctatt tggtgatata tgggagatgg cggtagaatg
    40501 acccgttttc aatcccaacg taagcaaaaa atacgatgaa tctgagtact aaacaaaaac
    40561 agcacctgaa aggtctggca catccgctca agccagttgt tctgcttggc agtaatggtt
    40621 tgaccgaagg ggtgctggcc gagattgaac aagcgttaga gcaccatgaa ctcatcaagg
    40681 tgaaaatcgc caccgaagat cgcgaaacta aaaccttgat cgtggaagct atcgtgcgcg
    40741 aaaccggcgc ctgtaatgta caggtcatcg gtaaaacgct ggtgctttat cgcccaacta
    40801 aagaacgtaa aatctcgctg ccacgctaag attatcctaa agttacacac atttgctgtg
    40861 taaaacgagg ggttttccgc aggcaggaga gcaaaatgcc acgctctgtt cgttgataaa
    40921 aggccgcata gcggcctttt tcctttcttt acaatacatc aacatcttga gtattgggta
    40981 attcttacag gtattccacc ttaattactt caaattctac ttcgccgccc ggcgttttga
    41041 tgaccacaac atcatcttct tctttgccga tcaggccacg agcaataggc gagtttacag
    41101 aaatcaggtt ttgtttaaag tcagcttcgt catcgccaac gatgcgataa gtctgttctt
    41161 cgtcagaatc cagattcagc accgttacgg tagcaccaaa aataacgcgc ccattgttgg
    41221 gcattttggt gacatcaatc acctgcgcgt tcgacagctt ggcttcgatg tctttaatac
    41281 ggccttcgca gaaaccctgc tgttcacgag ctgcgtggta ttcggcgttt tctttcaggt
    41341 cgccatgctc acgcgcttcc gcgatagcag caatgatttc aggacggcgc acagatttca
    41401 gaaaatccag ctcttcgcgt aatttttcag cgccgcgtaa ggtcatcgga atagcttgca
    41461 tttgttatac ctcttgaata ttcctgatgg ggcaagtctt cacccacccc ggctgttcgg
    41521 cctgcccggc ataactcctc accgggacca gaagcaaaaa aataccgacc cgggtacaag
    41581 tcccaggtca gctacaattc acattttgat agtcatttta ccctgaagtt cccgaagggt
    41641 catcgtttac tttatagggc gttgcgccgt agtatgacgg ctcgattcca ggttgttagc
    41701 gcgagattat gcgattttcc agatttatca tcggattgac cagctgtata gcgttcagtg
    41761 ttcaggccgc gaatgttgat gagtacgtta ctcaactccc cgctggtgcc aaccttgccc
    41821 tgatggtgca aaaagtcggc gcgtcggccc ccgctattga ttaccacagt cagcagatgg
    41881 cgctgcccgc cagtacgcag aaagtgatta ctgcgctggc ggcgttgatt caactcggcc
    41941 ccgattttcg tttcaccacg acgcttgaaa ccaaaggcaa tgtggaaaac ggcgtactta
    42001 agggtgactt agtggcgcga tttggtgccg atccgacgtt aaaacgtcag gatattcgca
    42061 atatggtcgc gactttgaaa aaatctggcg tcaaccaaat cgatggcaat gtgttgatag
    42121 atacctccat tttcgccagc cacgataaag cccccggctg gccatggaat gacatgacac
    42181 aatgctttag cgctccgcct gccgctgcca tagttgaccg caactgtttc tccgtctcgc
    42241 tctacagtgc cccaaaacct ggtgatatgg cttttatacg cgtggcatct tattaccccg
    42301 ttacgatgtt cagccaggta cgcaccctcc cccgaggttc tgccgaagcg caatactgcg
    42361 aactggatgt cgtaccaggc gacctgaacc gctttacgct gacgggatgc ctgccacaac
    42421 gttctgagcc gctcccgttg gcttttgccg tgcaggatgg agccagctat gccggggcaa
    42481 ttctgaaaga tgagttaaaa caggcaggta tcacctggag cggaacactg ctgcgccaga
    42541 ctcaggttaa cgaacctgga acggtagttg ccagtaaaca gtcggccccg ttgcacgatc
    42601 tgcttaagat tatgctgaaa aagtcggaca acatgatcgc cgatacggtt ttccgcatga
    42661 taggccatgc gcgcttcaat gtgcctggaa catggcgggc cgggtcggac gccgtgcgtc
    42721 agatcctgcg ccagcaagcc ggtgtcgata ttggaaacac cattattgcc gatggttcag
    42781 ggctttcgcg gcataacctg attgcccccg ccaccatgat gcaggtgttg caatacattg
    42841 cccaacacga caatgaactt aactttatct ccatgctgcc gctggcgggc tatgacggct
    42901 ctttgcagta ccgtgcaggt ctgcatcagg cgggcgtgga tggaaaagtc tcagcgaaaa
    42961 ccggttcgtt gcagggggta tataacctgg cgggattcat taccacagcg agcgggcaac
    43021 gaatggcgtt tgtacaatat ctttctggct atgcagtaga accggcggat cagcgtaatc
    43081 gccgtattcc gttagtgcgt tttgaaagcc gtttgtataa agatatttat cagaacaatt
    43141 agtcaaaaag aaaccccggc acatggccgg ggttttgctg attaacgctt gtaaatgaac
    43201 tcaacgcctt cttcgtcgtc ttcgtcccag tcatcatccc agtcctcatc atcctcttca
    43261 gcaatctctt caagctgctg gcgatgataa tcatcccaca tgaattcgac tttctctggc
    43321 tgtttcgctt cttcagcctg cacgaccggg ttttcaatga taaaggtcat cacatcccag
    43381 cagagatctt tcacgcccag tccactcgcc gcagagatca gataatattt atcttcccag
    43441 cccagcgctt cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg
    43501 atcttattga acactaacca acgtggtttc gccgccagat cctggctgta tttttccagc
    43561 tcgctgataa taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg
    43621 atgaggtgca acaggacgcg gcaacgttcc aggtgcttca ggaagcgaat gcccagacct
    43681 gcgccttccg cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg
    43741 ttgtccattc gtaccacacc cagacttggc accagagtgg taaacggata atccgccact
    43801 ttcggtttag ccgccgatac cgcacgaata aaggtcgatt tacccgcgtt tggcatcccc
    43861 aacataccga cgtcagccag cagcatcagc tccagcagca gctcacgctt atcgcccggc
    43921 gtaccgttag ttttctgccg tggtgtacgg ttaactgacg atttgaaacg ggtattgccc
    43981 agaccgtgcc agccgccctt agcaaccagc agacgctgac cgtgtttggt catatcgccc
    44041 atggtttcac cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aattgtgacg
    44101 tctttaccgc gcttaccggt acagtcgcgg cttgcgccat tctgaccgcg ctctgcacgg
    44161 aaagattttt caaaacgata atcgataagc gtgttcaggt tctcgtcggc ttccatccat
    44221 acgtcaccac catcaccgcc gtcgccgcca tccgggccgc ctttcggaat atacttttcg
    44281 cggcggaagc tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca
    44341 tcaacaaact tcattttatt ctccgtaaat cattcgcctg cgcggggttg cgataccacc
    44401 gttgtatgct tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc
    44461 gcaaccacga caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa
    44521 tcgggccagg ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg
    44581 atgatcgcgc tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat
    44641 cctaccaagg tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc
    44701 gctatcactc caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa
    44761 aacaggatct gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc
    44821 acccaaaccg tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt
    44881 gtaaatatct cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat
    44941 gcccctacaa cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc
    45001 atgccaactg gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag
    45061 gatgagctga acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc
    45121 aaccagcgtg gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg
    45181 gcaccaaggc caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc
    45241 acctccagca cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc
    45301 gccaaaagaa tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt
    45361 gcggattaac gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa
    45421 cgtgttgcgg ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg
    45481 ataaatttac ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca
    45541 aacagagtgt ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt
    45601 tgacgaacga tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg
    45661 cgtttagctt ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt
    45721 tgaaatctct cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact
    45781 gacgatggcc ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt
    45841 tctcgccacg accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa
    45901 cgccgatttt gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt
    45961 cgccagttgc gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt
    46021 gtttaccacc actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt
    46081 caatgattca gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca
    46141 cgcctttgac aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac
    46201 gtttatctgt gccatttttt cagtacaatc actctatatt cctaaccata aaccctaagt
    46261 tgcctttgtt cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga
    46321 aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca
    46381 gcttaattcc gacgtccagc tgatcaatca gttaggctat tacatcgtca gcggcggcgg
    46441 taaacgtatt cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa
    46501 tgcgcatgtc accattgctg ccctgatcga gtttatccac acggcgactc tgctgcacga
    46561 cgacgttgtg gatgaatcag atatgcgcag gggtaaagct accgccaacg ctgcatttgg
    46621 caatgccgcc agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac
    46681 cagcctcggt tcgctcaaag tgctggaagt catgtcagaa gccgtaaacg tcatcgcaga
    46741 aggtgaagtt ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat
    46801 gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat
    46861 tctggctggc tgtacgccgg aagaggagaa aggcctgcag gattatgggc gctatctcgg
    46921 cactgctttc cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg
    46981 taaaaatgtc ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat
    47041 gcatcatggc acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg
    47101 tcgccatctt ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac
    47161 gcgtcagcgt gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga
    47221 caccccttgg cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta
    47281 atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac
    47341 taattcagcg ctataacacc tgaaaaaaag ggcgatgaat ctttatatat tctgaatatt
    47401 cacactcttt acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac
    47461 agaagaagtg ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat
    47521 ttcattgact ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg
    47581 gcggcggagt ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc
    47641 ccatggccga aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc
    47701 tggccatcac gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg
    47761 cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc
    47821 cagctctcag acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt
    47881 ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc
    47941 ccttccgcaa tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata
    48001 acctgtgcgc cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg
    48061 gcatgcgcgc ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt
    48121 tcggtgatga atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata
    48181 tcggtcggga atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca
    48241 tgcatatcca ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt
    48301 ttcgccagca cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga
    48361 gaaatcgccg ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag
    48421 acaccgccgc ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta
    48481 attttcgcac ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct
    48541 gcgttttcaa taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt
    48601 gcgccaacgc tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa
    48661 gctttaacgt aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg
    48721 tgtagatcaa ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga
    48781 ccaaagcgcg ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga
    48841 tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt
    48901 tctactttcg cccccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt
    48961 gggacgttct ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga
    49021 gcagcatttt tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca
    49081 cgaaatttat ccatttagtt tgttctcagt taacaattca tatccgctac cggctaatcg
    49141 cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct
    49201 ttgatcgaca cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc
    49261 gtctgctgtt ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga
    49321 aagtggctgc catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg
    49381 ctctgaattt cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca
    49441 tcttagagca aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa
    49501 ggctcgactt gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa
    49561 atttaacgag gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg
    49621 tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg
    49681 agcagcagtg ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta
    49741 atccccttca ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc
    49801 tctccggata acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt
    49861 ttttctcttc cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt
    49921 cgataccttt ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga
    49981 cgccttcagc aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact
    50041 ggaagtccag acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca
    50101 caatggtttt atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca
    50161 tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt
    50221 cacgttgagc aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt
    50281 tcacctgtac gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg
    50341 cccgaatttg cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca
    50401 tcagcttata cggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca
    50461 gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg
    50521 tacccacagg ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat
    50581 tcttattgtc atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca
    50641 tcgcagactt agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg
    50701 ggtcttcaaa accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa
    50761 tgctcagcga actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc
    50821 gcggcagata ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac
    50881 caatactgac cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg
    50941 tgtaggtcgg ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg
    51001 ccgccagcag tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca
    51061 tgaactcaat tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga
    51121 gacgagtgga caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggcg
    51181 tcgtagccgt taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc
    51241 agacagttga ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac
    51301 ccgctatcaa tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg
    51361 acgaaaataa ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata
    51421 acccgacgca gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc
    51481 atcaggccga tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca
    51541 accaccggcc ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc
    51601 gcactataag tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg
    51661 ccagaaacca caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc
    51721 agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg
    51781 gcccgcccga acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt
    51841 aacagcatga gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata
    51901 gcggaacgga acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg
    51961 attcgcctgc aacgcctggg cactgccatg agcgataatt tttttgtccg ccaggatcca
    52021 ggcgtgatcc gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac
    52081 gcccagcgcg ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc
    52141 ttgcccaaca aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc
    52201 cagcgctgca cgccgcgcca tcccaccaga aagttcagaa ggcattagct tagccgctcc
    52261 acgcagcccc acggcctcca gcttcatcat cactgtacta tgcaacaatg gcgcgggaag
    52321 ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa
    52381 cgccccggac tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga
    52441 catcgccgga atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc
    52501 gccaatcaga cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat
    52561 cttccctcgc ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa
    52621 actgacatcg cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc
    52681 ttgcgttgat tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa
    52741 tattacccgc cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc
    52801 aaaattaaga atccgttacg gctttctgaa aatcttcagc ggaccggcga gtatacctga
    52861 agaaaggatg ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg
    52921 tttacagtgc cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc
    52981 cgccgctgat catcggcatg acggtggtca gtattggtac atcgttacca gaagtcatcg
    53041 tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct
    53101 caaacattat caatatattg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg
    53161 tccattctga tgttctacgc cgtgaattac ccttaatgtt gttggtcagc gtggtggccg
    53221 gttccgtact ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg
    53281 ctgtgctatg gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg
    53341 acagcctgac cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt
    53401 ttttatggct cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata
    53461 acgccacggt gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa
    53521 ttgctatcgg aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg
    53581 aaaacgacat tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt
    53641 tgggtttacc cgcgctgata acgccaggag agattgatcc actggcgtac agtcgtgact
    53701 acagcgtgat gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccac
    53761 aaccgggccg tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt
    53821 tgtactggtt atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag
    53881 ttacaaccgg gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa
    53941 tgcctggcgg agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg
    54001 ttctggtgta aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc
    54061 aaaatggccg caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa
    54121 gccgcgcatg gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac
    54181 tcaggtgaat ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg
    54241 ttaatctgca tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg
    54301 tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc
    54361 gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact
    54421 gctgaagatt ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc
    54481 gtaaacgata ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg
    54541 cgtgacgcat tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac
    54601 aatatgatga ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc
    54661 gtggatgttc gtcagttaag tattgccgat gtgatgacgc cggggggaat acgcgtgcgc
    54721 cctggcattc tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg
    54781 atggttgccg atggcgacca tttactcggt gtgttacata tgcatgattt actgcgtgca
    54841 ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga
    54901 cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc agagaacatt cgtctgctga
    54961 tcctcgacgt tgatggcgta ctgtcagatg gcctgattta tatgggcaat aacggcgaag
    55021 agctgaaagc gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata
    55081 ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat
    55141 tggggatcac tcacttgtat caggggcagt caaataaact gatcgccttt agcgatctgc
    55201 tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact
    55261 ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga
    55321 tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt
    55381 gcgacttatt actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag
    55441 taaagccaga cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat
    55501 taatatggcc gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta
    55561 taaaagcgag catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt
    55621 gattgctcaa cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt
    55681 acttaccacg tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa
    55741 gctgaccaat gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc
    55801 ggactctcaa cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga
    55861 tgttacctct gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa
    55921 aatgcgcggc aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta
    55981 tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg
    56041 ccagcattcc ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg
    56101 accagcaatc tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca
    56161 cccagggcac catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac
    56221 aaggtaaaga agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggata
    56281 acggtaaacc ggttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg
    56341 tcgttctgac gggtaatgct tatctgcagc aggttgatag caacattaag ggcgataaga
    56401 tcacttacct ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa
    56461 ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac
    56521 agaagaaggg taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct
    56581 ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg
    56641 gtctgctggg gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg
    56701 tgccgcgcga tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc
    56761 atgcacgcgc gcgccgcgga atcggctatc tgccacagga agcctccatt ttccgtcgcc
    56821 tcagcgttta cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac
    56881 aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca
    56941 gcatggggca gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg
    57001 ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg
    57061 ttatcgacat taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca
    57121 ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg
    57181 ggcatttgat cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg
    57241 tataccttgg ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga
    57301 gagtacgatt ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat
    57361 gacgccacag ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca
    57421 ggagctacag caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga
    57481 aatcgacacc cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca
    57541 aaaagagatg ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg
    57601 tacaccatcc ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga
    57661 aacgacgcag accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga
    57721 cactgaccgc gctattgcta cctctatcgt cgatgccgtt gatgacaccg gttatctgac
    57781 tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt
    57841 tgaagccgtc cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct
    57901 gcgtgactgc ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga
    57961 ggccagactg atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt
    58021 aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc
    58081 gctcgatccg cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga
    58141 tgtgctggtg cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc
    58201 gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga
    58261 cagccagttt atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag
    58321 ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt
    58381 tgagcaaggt gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga
    58441 aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg
    58501 catttttgaa ctgaagtatt tcttttccag tcacgtcaat accgagggcg gcggcgaagc
    58561 ttcctccacg gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa
    58621 accgttgagc gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg
    58681 ccgcactgtt gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca
    58741 gctcgtttga cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg
    58801 tcgagatcac cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat
    58861 attttgaccg aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct
    58921 cagatgcaac actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca
    58981 tgtacgctgc cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag
    59041 ataaactgaa acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc
    59101 ggcacaacgg gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca
    59161 ggttcttagg tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta
    59221 acagggaatg tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca
    59281 tcagcgagct ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc
    59341 tgacgcggga aaaaatgggc agtaccggta tcggcaatgg tattgcaatt ccgcatggca
    59401 aactggaaga agatactctg cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag
    59461 ctttcgatgc catcgacaac caaccggtgg acttactttt tgccctgctg gtgccggcag
    59521 accaaactaa aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa
    59581 ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg
    59641 ataccgaagg tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg
    59701 aggagaaaca gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg
    59761 tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt
    59821 tgttacccga tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg
    59881 atgttcgtaa tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg
    59941 acgctttctc accgcaacta ctgttcctgg atgccgaccg taatacctta attcgtcgtt
    60001 acagtgacac gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta
    60061 tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata
    60121 cctcagaaat gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac
    60181 gtgaacgcga actgacaatg gtctttgagt ctttcggctt caaacacggt atccctatcg
    60241 atgcagatta cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc
    60301 gtccaatgac aggtcttgat aagcccgtcg ccgcgttcct cgaccgccac acagaagtac
    60361 acaattttat ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca
    60421 acaaccgtag ctacctgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt
    60481 atattgcaga gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc
    60541 atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa
    60601 gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc
    60661 tgaagtgctg ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct
    60721 gctgatgtta gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga
    60781 agaggaagca ctggcagccg ttatcgccct ctttaactct ggttttgatg aagattaatc
    60841 ttcatcactt tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac
    60901 tgaaatcgtt ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa
    60961 ccttaagaaa accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg
    61021 agatcgtcag aaaatatttc caggagatgg catgattcgc ttatctgaac aaaatcccct
    61081 gggcaccgga agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa
    61141 gattgtctac catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta
    61201 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    61261 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaacc
    61321 ctccattacg ctgaccgaat ttgctgagca atgccgttac gaagaagaca ttgcacaact
    61381 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    61441 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    61501 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    61561 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    61621 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    61681 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    61741 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    61801 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    61861 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    61921 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    61981 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc
    62041 cgtctgctga gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc
    62101 cagggctttc tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc
    62161 tgcggcaatc accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga
    62221 atgtgccacg taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac
    62281 cgctgagaag ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc
    62341 gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct
    62401 tttactcatt cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct
    62461 tctgcaatgt tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca
    62521 accacgatat catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct
    62581 gcggtatcga tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc
    62641 atcgctgggg caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc
    62701 agcgccttta attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa
    62761 ttcttcgccg cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc
    62821 tgggccagag gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc
    62881 gtttccgtca tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt
    62941 gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca
    63001 tgaatttcag aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt
    63061 gtgatcatcc tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca
    63121 tcacacattt aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc
    63181 ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag
    63241 atttccctgg tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt
    63301 ggcttttgcc acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc
    63361 ttcaatccat tcaccgacgt tatcttccca ggcaggaagg tccggagact gaatttgctg
    63421 acccagctgt tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc
    63481 ttcctctaca atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata
    63541 gcccggcatc atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag
    63601 ttcgagatac tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc
    63661 tgtcgtcacc gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt
    63721 tagcgccgga accgaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc
    63781 ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg
    63841 ataacgcttc gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc
    63901 cagcaacacc aggtcgtatt cgcccggttt aaacatctcc agcgccgcct tgccggtcat
    63961 ggcgacatca acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag
    64021 ttcaatgtct tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa
    64081 cgcatcatcg acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc
    64141 tttgccctgt tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga
    64201 aacggccaga ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg
    64261 gtaatacatg gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc
    64321 cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg
    64381 ctgggtgaat ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc
    64441 gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt
    64501 ttgttgcgcc tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac
    64561 cggttgatta tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt
    64621 aaagatattc cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc
    64681 ggcggtgagt tcggtatcca gcagaatacg gctcaggccg acgataccgt tcagcggtgt
    64741 acgcaattcg tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag
    64801 cgcatcctga taccgcttac gctcggtaat gtcgcgacca aagcccatca aaccgtgacg
    64861 tttacccacg cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc
    64921 cgggtaatcc agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc
    64981 ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac
    65041 cagttgtttt tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc
    65101 tttatcttcg ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa
    65161 tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag
    65221 ttggccgaag gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt
    65281 tagctgggca atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc
    65341 ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac
    65401 caccgataga aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat
    65461 aacatcaatg ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat
    65521 ggcaagaacg acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat
    65581 catcaggtca acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca
    65641 cgacaacctg ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat
    65701 gcggaattcc cctcaacgac ctactccgtt ggaggtatcc acggacgttc cagcgccgat
    65761 ccctgtacgc catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt
    65821 tcgcaccaca gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag
    65881 atcacttccg gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt
    65941 tcaataccgt tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata
    66001 tgcagcggat gcagctttat gccgtccacg ccggtttcaa ccactcgctc cagcgtttgc
    66061 aaacattcag cctgcccttc gccaggcagg ccgacaatta agtgggaaca caccttcagc
    66121 ccgcgctgac gtgccagctg ggttgtacgc tgataacagg caaaatcatg gccgcggttg
    66181 atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca gctccagcca cacttcgtaa
    66241 ccctggtcct tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgg
    66301 gtaccaacac acaaaccgac aatattggcc tggctcaccg cctgctgata catagaacgc
    66361 agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa agtaggccag atagcgttta
    66421 gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg atgctgctgc
    66481 gcttcatcgg caaacgaggc aacattacag aatgtgcagc caccacgccc gatggtaccg
    66541 tcacggttag ggcagctaaa accgccgtgt agcgtcagct tatgcacctt ttgcccataa
    66601 cgacgggtga gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag
    66661 accgcgcctt gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg
    66721 atcaatcgtc ccggcctgct tttatcaact gcataatcaa tcaaaattac cgaaatttca
    66781 tgcataatca cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg
    66841 attttaggca aaaacagtag catgaaacgt cattaccaat taaggcagta taaaatgctg
    66901 gttttctcgt cagttcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt
    66961 gactgtttct ctaacgactt cccttttagc cttaaagata aaatccattt taatttcagt
    67021 aatttaataa agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac
    67081 ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg
    67141 ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag
    67201 gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct
    67261 ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca
    67321 cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca
    67381 catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat
    67441 gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt
    67501 acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa
    67561 aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg
    67621 ccgcatcgtt gaagaagagc tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt
    67681 ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgcccc acattgagca
    67741 aattttcgtt aacgctccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat
    67801 cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct
    67861 gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta
    67921 tctggatctt gcggacctgc gtctggaatc ggccatctgc ctgttccacc agcgcttctc
    67981 cactaacacc gtaccgcgct ggccgctggc gcagccgttc cgctacctgg cgcataacgg
    68041 tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca
    68101 gacgccgctg atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggttctga
    68161 ctccagttcg atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg
    68221 cgccatgcgt ctattagtac cacccgcctg gcagaacaac ccggatatgg acccggaact
    68281 gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat
    68341 cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc
    68401 acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga
    68461 ttatcagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat
    68521 cgacacccgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg
    68581 ccatccatat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct
    68641 gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa
    68701 acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg
    68761 tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc
    68821 gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga
    68881 cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt
    68941 cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta
    69001 ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga
    69061 tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa
    69121 agccgaaaaa atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc
    69181 taaagatcgc ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt
    69241 cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc
    69301 gcaccacttc gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta
    69361 tgaaacgctg ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat
    69421 gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat
    69481 ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tgcacgatga
    69541 tgtagtgggc ctgtgcttcc agggggcggt cagccgcatt ggtggggcga gctttgaaga
    69601 cttccagcag gatctgctga acctgtcgaa acgtgcctgg ctggcgcgta agcccatcag
    69661 ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcttaca acccggacgt
    69721 ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac agcgactatc aggaatacgc
    69781 gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg
    69841 tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga
    69901 taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc
    69961 gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta
    70021 tggcactaac aaagtgtcgc gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc
    70081 ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg
    70141 cgaaggcggt cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc
    70201 ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga
    70261 cttagcgcag ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa
    70321 gctggtttcc gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga
    70381 tttaatcacc atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa
    70441 atacgcaggt tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa
    70501 tggtctgcgt cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat
    70561 catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc cgatggtggc
    70621 gctcggctgt aaatatctac gtatttgcca tctgaacaac tgcgcaacgg gtgtagcaac
    70681 tcaggatgac aaactgcgta agaaccacta tcacggcctg ccgttcaagg ttaccaatta
    70741 ctttgaattt attgcccgtg aaacccgcga gctgatggca cagctgggcg taacgcgtct
    70801 ggtggatctg attggtcgca ccgacctgct gaaagaactg gacggtttca ccgccaaaca
    70861 gcaaaaactg gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact
    70921 ctactgcacc gaaaacaacc caccgtttga taacggcctg ctgaacgcgc agttgctgca
    70981 acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atattcgcaa
    71041 caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca
    71101 ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg
    71161 tgtgtggaac gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg
    71221 taaaggcatg gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag
    71281 ccatgaagca agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta
    71341 tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt
    71401 agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg
    71461 taaaaccggc gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga
    71521 aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga
    71581 tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg
    71641 ctctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct
    71701 ggttaaaccg aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc
    71761 agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt
    71821 tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt
    71881 tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct
    71941 gtcgtgcggc aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg
    72001 gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa
    72061 caccctgccg gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg
    72121 cactctgaac gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga
    72181 taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa
    72241 agtggcgatt atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa
    72301 cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg
    72361 tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg
    72421 catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct
    72481 gctgagtgat tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg
    72541 gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac
    72601 caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa
    72661 acgcgtggtg gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg
    72721 ccagggagcg aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc
    72781 ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc
    72841 actggggatt gaagtgaacg gtaacggtaa agtcagcggc gtaaaaatgg tacgtaccga
    72901 aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca
    72961 tatcgttccg gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg
    73021 gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag
    73081 cgacaacgcc ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg
    73141 ttccgatctg gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa
    73201 ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
    73261 attctccttt ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
    73321 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
    73381 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
    73441 aatccacgac ggtattattc atctggtcga tagcgcccgg acggaaacgg tacgtagcgt
    73501 taacgcgctg atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca
    73561 aggtggcgag gccagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt
    73621 aagtctaagg tataagcgtg ggttctctgc aaaaaactta cgccaaatga ggctttttta
    73681 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
    73741 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
    73801 caaatctttc cccctgccct ggtccaccta cgtccgttta ctttccgtta aaaacgctga
    73861 cgcccgcagc ttttatgaaa aagagacgct ccgctgtggc tggtctgttc gccagctaga
    73921 acggcaaatt gcgacccaat tttatgagcg aacactactg tcacatgaca aatcagccat
    73981 gctgcaacaa cacgctccta ccgagacgca tattcttccg caacaggcga tacgcgatcc
    74041 ctttgtgctc gaatttctgg aattgaaaga tgaatattca gaatctgatt tcgaggaggc
    74101 gctgatcaac cacctgatgg atttcatgct ggaacttggg gatgattttg cctttgttgg
    74161 tcggcagcga agattacgca ttgatgacaa ctggtttcgt gtcgatctgc tgtttttcca
    74221 ccgccgttta cgctgcctac taatcgtcga tctaaaagtg ggcaaattca gctatagcga
    74281 tgccggacag atgaatatgt atctcaacta cgccaaagag cactggacgc taccggatga
    74341 aaatccgccc atcggtctgg ttctctgtgc agagaaagga gccggagaag cacattatgc
    74401 tctggcaggt ttgcctaaca ccgttctggc aagcgaatat aagatgcaac tacctgatga
    74461 gaaacgactc gcagatgaac tcgttcgaac acaggcggtg ctagaggaag gctatagacg
    74521 ccgttaattt cagcggtttc gaattctgca gacagtgtct gcagaatctt taaaacgaac
    74581 taagttttaa gccattaaat cagccttaac cttcaccaca acctttttaa tctctccagg
    74641 ctcgccgaca acgcatcctg gtttatgcgg ttcacctggc ataaacacgg cgaacattcc
    74701 cggttttaag atgatggctt gctcgttatc aatggtgctg caaagctgat aatcatcctc
    74761 atggtggaac tcttcacact ggcgcgcagt gcctgccatg ccaaacagaa tccgttcctc
    74821 accgtttaat aacagttgga tatcaatgta ttgctcgtgc aattccgctt ttttctcgac
    74881 gggcgattga gtgttaaacg tcatgacatt cataaagata ttgtcgccct gtaattcgta
    74941 acgacccggc gctttttctt gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa
    75001 cgcaggatgt aacccagcag acggtaatga ctgtacttca cccatcatca taatttttct
    75061 ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt ggcggtagtg cgccgccagt
    75121 aaatcaacat gaaatgccgc tggctcctgc gccagatacg tttccaccag cgccagatac
    75181 ccttctgcca gcccaacgct gccaccgacc accacgcact ggcaatcagt tgtggcttta
    75241 atatcagcga tcagccttgc aagcgtacgt gcggagcggt gaatcagctg ctgcgcctgc
    75301 tcgtcgccct gcccggcgcg tgtgaaaata gttctcgcat ccgcgcccgc cagttcaccc
    75361 tgcgctgccg ccgcaatgcc gcgaccagaa gcaatcgcct ccacgcaacc tgtgcgtcca
    75421 cagccacaga ccgggccgtg tggatcggca agcgtatgcc cgatatgccc cgccagaccg
    75481 ccagggccgg taagcagttt gccgccgctc actacaccgc cgccgacgcc agtggaaacg
    75541 gtgataaaca ccatctcggt tacatcgcct tccagcgcct gatactccgc ccatgctgca
    75601 gcctgcgcgt cgttaatggc aatggtcggc aaatcggtaa gttgttccag cgttttgact
    75661 aacggaaagt gtagcaatcc accaagatta tgcggattca gcgccagcaa gctgccgtcg
    75721 cgaataatcc cggttgaagc gatggcaacc cgctgcgcat gagcttgcaa cggagagact
    75781 aatgcggata aggcatcacg caaggcttct ggtgtctggc tggctggcgt aggaagttca
    75841 cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg
    75901 atatcaatcg ccagcgtggt catagcaccg cctttttcat cgctgtgttg taccactgac
    75961 aaatgtgttc aagacgcgta atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca
    76021 tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc ctacatccgg
    76081 cgtcgctcaa cgttttcacc agcgccagat ccggctcttc tggcgtttca ggcgtggtat
    76141 agccagaaag cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc aggccgtctt
    76201 ccggcgttga gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt gccagcagcg
    76261 tttcaacagg caccggacgc gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg
    76321 cctgcgccag cgcatcgaca tcttcaatat aggccgtgat gcgtaccgga gaatcctcca
    76381 gatcgcgttt cacaatcccg ataattggca cgctcaccac cgcacgcgtg gcttgcagat
    76441 ttgccacacc ttcaatgcga atggcaaccg cgcccgcctg ctctgccgct aatgccatgg
    76501 cggcgacgat ttcgggttta tcgagcggac tgtccggaac cggctggcag gagacaatca
    76561 ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag taacgacata catcttccct
    76621 tagcgaaagg cccggtacat agaccgggca acaggattaa cttttggttt tgactaaatc
    76681 gtttttggcg ctgccaaatg gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg
    76741 agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat
    76801 cagcaggatc accacgaacg tcagactgaa cgagagcgat gccagcgcag tacccagatc
    76861 cagacgttga gcgatcaacg cgccgatgat tggggccagt gcgccgccca atgcgccaac
    76921 gttgtaggta aagcccaggc ccgctgcacg ctggtcggta tcgaaataac cgccaatcag
    76981 tttcggtaag atcccggcga tcccttgtcc aagcatttgc tggaagaaca gtaacagacc
    77041 gagcacccag acgtttgcgc cgccaatcgc aaataccgga ataatcagca gctgcgaggc
    77101 cagcaggcta caaacgtacg ctttgcgggt tcccagccag tcaccgagga agccacctac
    77161 gcagcatccc acggccgcgc caaagccact aaagaacagc acattggcga cagtatgcgg
    77221 gttataagcc agatcggttt tcagatacgt tggcagcagc gcctgaatcg gccatgagta
    77281 gaggaaagca aacaacacga ccaccatcag cattacgccc gttggccagc gtttacccgt
    77341 actttgcacc ataaagctga taaagattgc ggcgcataac agcccgagaa cagcgacgat
    77401 tgcagcattt tgcaggttac cggcgaagca gaaccacagc gcagtagccg ccgccagtgt
    77461 cattacgata ttggcaatgc gatgttcacc acggtagaga atatccacca ttgtgcgtac
    77521 tggtgcttta cctgcgtgtt tctctttcca gtcttccgct tccgggatgt ttttacgcag
    77581 ccagagagca aagatgattg gcaaaatgcc gataaagaac agcgcacgcc aaccccagac
    77641 cggaaccacc aggctataga cctgagcggc aacgacggcc cccacagaga agcctgaaat
    77701 caaaaaacca ctggctttgt tacgcagatg ttttggccag ctttcaatga cataggtggc
    77761 gctggaaccg tattcacccg ccatccccat gccgatgacc agacgagcga taaacatggt
    77821 gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa cgatgctggt
    77881 gaccattgcc agacgacgcc cgtagcggtc acccatagcg ccgagcatca ggccgccgaa
    77941 ccagcgagag ataaaggctg cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa
    78001 ttcaccctgt acttcggtga gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag
    78061 atatcccaac caggcagcgg aaaatgcccg ccattgtgca cgattgagat ggcgatacca
    78121 cgggatattc tgggttgtag tactcattgt gagtctcccg cggtgggcga tgcccacacg
    78181 ctttggtatg aaaattgtag ggtacagatg cgtttatttc ccctcacccg gtaggggcga
    78241 gcgaggggaa acaactcacc cgcgctcttg catcaactgc tgggccagcg ccttcagttc
    78301 tggcagatat ttttcatcta ccggaccaaa cggtttgcgg cacagcggca cagaaacgac
    78361 atccatataa tgcaggacag ttttcaggcc gcggaatacg cccgttttga tcagtaaatc
    78421 aatgacttta ttacattcgg tttgcagctt ctgcgcggtc tggatatcgc cttctttcag
    78481 cgccttcaca atcccctgat aacgccagcc catgatgttg taggtactac cgataccacc
    78541 atcagcgccc gccagaagac cagaggcgaa gatttcgtcg taaccgttat agagcaccag
    78601 atcaggatgt tcacgacgga tctgctccat ctgatagaga tcgccagagg tctgtttcag
    78661 cgcacctacg ccaggcaacg taacaagtgt gttgatctga tccagggtca gttttacccc
    78721 actcagggct ggaatgttgt acaccaccat cggcaaacca tccgccgaat caataattgc
    78781 ccgatagtga tcgcagtgtt cttcaaagct gaaaggatag tagaacggcg tgacggcgga
    78841 gacggcatcg aagccataac gtttagccga tgccgcaagt tgttggcttt cggcggtgct
    78901 gacgcaaccg acgtgggcga tgagtttaat ctgacctttc gcctcttcgg cgacgatttc
    78961 cagtacctgt tcacgctcgg aaaggctttg tacaaaggcc tcgccggtcg aaccacccac
    79021 gtataaaccg tcgatgccct gctgaatatt gaactgaacc aggcgacgca gactcgcttt
    79081 atccagtgct tgttgttggt caaaaggagt caggagtgca gccattacgc cacgtaaatt
    79141 cgttgccata aatacctctg aagtgatgct tgtctgataa acgatatacc tttatacctg
    79201 ttataccaga tcaattaagc aacaccccat acagaaagct tataatgcga tctgcttcac
    79261 taaagtggca ttatttcttt ttgttggtgg tctgaccgaa ggcgtgccag gtagcagaga
    79321 cgctgttgag atgcgattgc aacgcacgat cggcttcgtc aggatcgtgg cggcggatcg
    79381 cgtcaacgat cgcaatatgc tgttgatagc taacgttgtt atgctcgtgc agagcctgat
    79441 cagccacggt tggacgtgcg gcgattagcc agtcgagcag ggcaacgtgg atcgccatga
    79501 agattgggtt acctgggatc tccgccagca cgcggtggaa atcaacgtct gaacgaatga
    79561 atgccgcgtt gttatccagc gactgactgt tgatttccag tgcttttgcc agcaaatcga
    79621 tttgctcatc ggtggcgtgt tcagccgcat agcgcaccag gctggattca aagaacagac
    79681 gtaattgttc gaaatgggca atcccaccag gatgagaaag gaaatctttc gccatgccgg
    79741 aaagctcacc gatgatagtg tccgcagaag gacgcgagac gcgagcgcgt tcgccgttgt
    79801 ttatttgcac cagacctttg cgttttaacg ctgccagcgc ttcgcgcaca gaaggacgcc
    79861 cgacgttaaa gaacgccatc agttcgcgtt cagacggtaa ttgttcacct tcgccaaatt
    79921 cacgacggcg gatcatctgt tccagctctt cttccaccat ttcggagagt tttttacgcg
    79981 ccagcgggcg gctacgcaag ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat
    80041 caaatgcgtt cataaggccc attctgtaag gtcagtgtga ttaacatcat cagtgacatc
    80101 ctatcacagg attgaaagta ggggaaaatg gcaggttttt ctctttgtgc ctcatcatta
    80161 ccataattaa cggaataatt aactattgcg aaaaattaat gtaacgcaga taaaaacatc
    80221 ccgtttgaat tattcataag actattcacg agcattatga atattatgaa tgtgttctta
    80281 caaaataatc ataagcgcat attttttaat gaaaaaccac ctcacctaca attaaaaaca
    80341 cgacatccgc accataaata gacttgcaaa aaatataaca tcattgtttt caatctgccg
    80401 tttatgggat tgaccgtttt cttttgacac ggagttcaac aatgttcggc ataattatat
    80461 ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa cctcaggtgg
    80521 tgctggctgc cgcaggtatc ttcctgatga tgtgcggtgt ctggttaggg ttcggtggtg
    80581 tactcgatcc cgccaaaagc agcggctact tgatcgtcga tatttataat gaaatcctgc
    80641 gcatgctgtc caaccgcatt gccggattgg ggctgtcgat tatggcggtg ggcggttatg
    80701 cccgctacat ggagcgcaca ggagccagtc gcgcgatggt gagcttgcta agccgcccgt
    80761 taaaactcat tcgctcgccg tatattattc tgtcggcaac ttacgtcatc ggccaaatca
    80821 tggcgcagtt tattaccagc gcctccggcc tgggtatgtt gctgatggtc accttatttc
    80881 cgacgctggt gagtctggga gtaagtcgcc tctctgcggt ggcggttatc gcaaccacga
    80941 tgtccattga gtgggggatt ctggaaacga actccatttt tgcagcccag gtcgcgggaa
    81001 tgaaaattgc cacttacttc ttccactacc agcttccggt cgcctcttgc gtcattatct
    81061 cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga caaaaaagat aaaaatatca
    81121 atcacgaaca ggcagagcta aaagctctcg ataatgtccc gccgctctat tacgccattt
    81181 tacctgtgat gccgttaatc ttgatgctcg gctcgctgtt cctcgcccac atcgggctga
    81241 tgcagtcaga actgcatctg gtggtggtga tgttactgag tttgacggtg acgatgtttg
    81301 ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga cgatgtgcag gcgttttttg
    81361 acggcatggg tacgcagttt gccaacgtgg taacgctggt ggtcgcgggt gaaatatttg
    81421 cgaaaggctt aacgacgatt ggcactgttg atgcggttat caggggtgcg gagcattctg
    81481 gtctgggcgg tattggcgtg atgattatta tggcgctagt cattgccatt tgtgccattg
    81541 tgatgggctc tggcaatgcg ccgtttatgt catttgccag tcttattccg aatatcgcag
    81601 ccggactaca tgtaccagcg gttgtaatga ttatgccgat gcattttgcc acgacgctag
    81661 cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac gtcaggaatt gcaggcgttt
    81721 cgccttttgc ggtggtgaag cgaacagcga tccccatggc agtcggtttc gtggtgaata
    81781 tgattgccac aatcacgcta ttttattaag ccattaaaag acaaaacagg ccaccagggc
    81841 ctgtttttgt attacttcac aacgcgtaat gccggtcgac caccgcgtgg tggctgcgga
    81901 ggttcatcgt caggatgagt gtcatcatcg tgatctggct tgtcgccatc aataaccgac
    81961 ataacggttt cgttgtctgc cgatgcctct tcatcattca tgatgctggt atcttcatcg
    82021 taggcagctt caggctcaaa catcgtgcct gcgccatttt cacgggcgta gatagccagc
    82081 acggcagcca gcggcacaga aacctgacgc ggaatgccgc caaagcgcgc gttaaagcgt
    82141 acctcatcat tcgccagttc cagattgccg acagcacgcg gcgcaatgtt gagtacgatt
    82201 tgcccgtcac gcgcatattc cataggaacc tgcacgccag ggagcgtcac atccaccacc
    82261 aagtgcggcg tgagctggtt atccagcaac cactcataga atgcacgcag cagataggga
    82321 cgacgtggtg ttagctgtga caaatccata cagattaact ccggcccaga cgcatttcac
    82381 gttctgcttc agttaaagaa gcaaggaaag agtcacgctc aaagacgcgg gtcatatagc
    82441 ctttcagctc tttcgcaccc gggccgctga actcgatgcc cagttgcggc agacgccaca
    82501 gcagcggagc aagatagcaa tcgaccaggc tgaactcatc gctcaggaag tacggcttct
    82561 gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag ttgcttacgt gcggcatctg
    82621 cttcagaagc tgaaccgttg atgatggtgt tcatcagcgt gtaccagtct ttttcgatgc
    82681 gatgcatgta cagacggctt tcaccgcgag ctaccgggta aacaggcatc agtggcggat
    82741 gcgggaaacg ctcatccaga tattccataa tgatgcgaga ttcccacagg gtcagctcac
    82801 gatccaccag ggtcggaacg ctctgattcg ggttgaggtc aatcagatcc tgaggcggat
    82861 tgtccttttc cacgtgttcg atctcgaaac ttacaccttt ctcagccagc acaatgcgga
    82921 cctgatggct atagatgtca gtaggaccgg aaaacagcgt cattaccgaa cgtttgttgg
    82981 cagcgacagc catgaaaacc tccaggtata gtcagaattt ttactgctac cagccaccag
    83041 gtggccagtc agaagttgtg ttacccagta aggaacgact ctctttgttc gaaaatcaaa
    83101 caaaaaatga gcaatacccg acatttgggc agaaaattgg atgatagttt accagatttt
    83161 gcgacctttg tggtgagtcg atgccggaaa tggggaaaaa gagatgcgct ttagtctgaa
    83221 atagttgact tagtccctta ttggcgatgt ggtttttgtt ttgcctgtct gtcagacggt
    83281 agcaaaaagc aactttccaa tttttacgct gattcagaat ttaaccataa aaaaacccgc
    83341 cgaagcgggt tttttcgcaa attgttttct gccggagcag aagccaatta acgtttggag
    83401 aactgcggac gacgacgtgc tttacgcaga ccgactttct tacgttcaac ctgacgagcg
    83461 tcacgagtaa cgaagccagc tttacgcagt tcagaacgca gggactcgtc gtattccatc
    83521 agagcgcggg tgataccgtg acggatcgca ccagcctgac cagagatacc accaccttta
    83581 acggtgatgt acaggtccag tttctcaacc atgtcgacca gttccagcgg ctgacgaact
    83641 accatgcgag cagtttcacg accgaagtac tgttccagag aacgttggtt gattacgatt
    83701 ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac ttttgcggcg accagtgccg
    83761 tagtattgat tttcagccat tgcctataat cccgattaga tgtcaagaac ttgcggttgc
    83821 tgtgccgcgt ggttgtgctc gttacccgcg taaactttca gtttacggaa catagcacga
    83881 cccagcgggc cttttggcaa catgccttta accgcgattt caatcacacg ctcaggacgg
    83941 cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac cgatgtggcc ggtgtggtga
    84001 tagtacactt tgtcagtacg cttgttgccg gttacagcaa ctttgtcagc gttcagaacg
    84061 atgatgtaat caccggtatc tacgtgcgga gtgtattccg ctttgtgctt accgcgcagg
    84121 cgacgagcca gttcagtagc cagacggccc agagttttac cggtcgcgtc aacaacatac
    84181 cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt tcattaaaag cttacccaat
    84241 aaatagttac acgttggtga acacccaaac gtcttcaatt gttgaggttc acacgacaaa
    84301 gtccggcaaa cctacccctt cgaatagcct atgccagcac acaaaaagtt ttgggaaaaa
    84361 aactttcttg taacgtgggg tcgcaggatt atagagaagt cggggtcaaa gatcgacccc
    84421 tttttgtgat ttgtgacagg ttttaacccg ccaaatgctc gcgcttcaga tactcttcgc
    84481 tttgcatctc ttgcagacgt gacaggcaac gctggaactc aaacttcagc cgatcgccct
    84541 gataaatttc atacagcggc acttctgcac tcaccactaa tttgacatgg cgctcgtaaa
    84601 actcatccac cagcgcaata aagcgccgcg cttcgctctc catcaaccgc gtcataactg
    84661 gtacatcaaa caacatgacc gtatgaaaga gacgtgagag cgcaatatag tcatgctgac
    84721 tgcgggcgtc gacgcacagc gtagtaaaag agaccgccag cgtctggttc tcgacgccca
    84781 ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga attttctcgt ttcgcccccg
    84841 ccagcgccaa ccatagttta tccatctgcg cccgggtttc atcgttgagt ggcgaaagcc
    84901 acagatgcgc ctgagtgagt gtacgcagtc gataatcaac accagcgtcc acgttcatta
    84961 catcacaatg ctgtttaatg gcatcgattg caggcagaaa acgcgcacgt tgcaggccat
    85021 ttcgataaag ttcgtccggc ggaatatttg acgtcgctac cagggtaata ccgcgggtga
    85081 acagggcttt catcagaccg ccaagtagca tggcgtcggt aatatcagaa acaaaaaatt
    85141 cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc ggcaataatt tccagcggat
    85201 cggtctgccc ctgtaaggca gttagctctt catgcacccg cagcataaaa cggtgaaagt
    85261 gcaggcgctg tttccgctct cccggcaggc tttgatagaa aaggtccatc agccaggttt
    85321 tcccgcgtcc tacaccgccc cacatatata agccacgcac tggcgtatgc tttgtgtctt
    85381 cgcgtttacc ccacagctta ccgacccgcg ccattagccc actcgtcctg ggggctggtg
    85441 gcgtgctatt gatgagttcc tgataaataa tttccaggcg gctgacggcc tctttttgaa
    85501 cgtcgtcggg ttgatggctg ccttcattga gcgccttcag gtattgcgat gttggggtaa
    85561 cgctttgcat gatcttattg ttattccttg aataatcggt gcgccgttgt tcacggttga
    85621 cgaaaaaaag gccgttctac actacgcgat atgcagtcgg gattccactt ctgtggaatt
    85681 aacggttata gtggcataat cagccgcagg catggagcct gaagccaaca ccctacggaa
    85741 acaaaagaca acgggagatg ttcatgacct gggaatatgc gctaattggg ttagtcgtcg
    85801 gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg taaactacgc cagcaacagg
    85861 cgttgcagta cgaactggaa aagaataaag ctgaactgga cgagtatcgc gaagagctgg
    85921 ttagccactt tgcccgcagc gcggaattac tggataccat ggcgcacgac tatcgccagc
    85981 tgtatcagca catggcgaaa agctccagca gcctgctgcc ggaactgtct gcagaagcaa
    86041 acccgttccg taatcgtctg gcagagtctg aagccagcaa cgatcaggca ccggtgcaga
    86101 tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg tactggcgcg aagcgcgact
    86161 aatttatttt tcgggcgcag ccattgcgcc ctcctcttct ctccctcccc gactatcatt
    86221 taatctggtg tctcattgtt agccgtctga aaattcaata acatcaaact gttttgaatc
    86281 tcttttctta tcattcaggt acgagagcag gaataatgaa aaaacaaacc cagctgttga
    86341 gtgcattagc gttaagtgtc gggttaactc tctcggcgtc atttcaggcc gtcgcgtcga
    86401 ttccaggcca ggttgccgat caggcccctc tccccagtct ggctccaatg ctggaaaaag
    86461 tgcttccggc agtggtgagc gtacgggtgg aaggaacggc cagtcaggga cagaaaatcc
    86521 cggaagaatt caaaaagttt tttggtgatg atttaccgga tcaacctgca caacccttcg
    86581 aaggtttagg ctccggtgtc atcatcaacg ccagtaaagg ctatgtgctg accaacaacc
    86641 atgtgattaa tcaggcacag aaaatcagta ttcagctcaa tgatgggcgc gagtttgatg
    86701 caaaactgat tggtagcgat gaccagagcg atatcgccct gttacaaatt caaaacccga
    86761 gcaaattaac gcaaatcgct attgccgact ccgataaatt gcgcgtcggt gattttgccg
    86821 tagcggtcgg taacccattt ggccttgggc aaaccgccac ctctggcatt gtttccgcat
    86881 taggccgcag cgggttgaat cttgaaggtc tggaaaactt tatccagaca gatgcttcca
    86941 ttaaccgcgg taactccggc ggtgcactat taaaccttaa cggtgagtta attggcatca
    87001 acactgcaat ccttgcgcct ggcggcggga gcgtcgggat tggatttgcc atccccagta
    87061 atatggcgcg aacactggcg cagcagctta tcgactttgg tgaaatcaaa cgcggtttgt
    87121 taggcatcaa aggcaccgag atgagtgccg atatcgccaa agccttcaac cttgacgtgc
    87181 agcgtggcgc gtttgtcagc gaagtgttgc caggttctgg ctcggcaaaa gcgggcgtca
    87241 aagcgggcga tattattacc agcctcaacg gcaaaccgct gaatagcttt gctgagttgc
    87301 gctctcgtat cgcgaccacc gagccgggca cgaaagtgaa gcttggcctg ctgcgtaacg
    87361 gcaaaccact ggaagtagaa gtgacgctcg ataccagcac ctcttcgtcg gccagcgctg
    87421 aaatgatcac gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
    87481 gcaaaggtat taaaatcgat gaagttgtca aaggaagccc agctgctcag gctggcttgc
    87541 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
    87601 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
    87661 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggcttacg tgtgatgtcc
    87721 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
    87781 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
    87841 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
    87901 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
    87961 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
    88021 aatcatggat caacgcggtt atatcatcac caataaacac gtaatcaacg acgccgatca
    88081 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
    88141 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccgat
    88201 caatgcacgt cgcgtaccgc acattggcga cgtggtactg gcgatcggta acccgtacaa
    88261 cctcgggcag accattaccc aggggattat tagtgctacg ggtcgaatcg gtctgaaccc
    88321 gaccgggcgg caaaacttcc tgcaaaccga tgcttccatt aaccacggta actctggcgg
    88381 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
    88441 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
    88501 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
    88561 acgcgagatc gcaccactgc acgcgcaggg cggtggtata gatcaactgc aagggatcgt
    88621 ggttaatgaa gtgtcacctg acggcccggc ggcgaatgcg ggtattcagg tcaacgatct
    88681 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
    88741 agaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
    88801 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
    88861 aaccggagtc tgtgctccgg ttttttatta ttcgctaatt aattacttat taacgaactc
    88921 ttcgcccagg gcgatatctt tcttcagcgt atccagcata ccttccagtg cgctctgttc
    88981 aaatgcgctc agggtaccga tagatttacg ctcttccacg ccgtttttac ccagcagcag
    89041 cggttgagag aagaaacgtg cgtactgacc gtcgccttca acataggcac attcgacaac
    89101 gccttgttcg ccctgcagtg cgcgtaccag agacagacca aaacgtgcag ctgcctggcc
    89161 catagacagg gttgcagacc cgccaccggc tttcgcttca accacttcag tacctgcgtt
    89221 ctggatacgt ttggtcagat cagccacttc ctgctcggta aaactaacgc caggaacctg
    89281 tgacagcagc ggcagaatgg taacaccaga gtgaccgcca ataaccggca cttcaacttc
    89341 gcctggctgt ttgcctttca gttccgcaac aaaggtgttg gaacgaatga tatccagcgt
    89401 ggtaacgccg aacagtttgt ttttgtcata aacaccggct tttttcagca cttcagcagc
    89461 aatcgcaact gtggtgttaa ccgggttagt gataatacca atgcacgctt tcgggcaggt
    89521 tttcgaaact tgctgtacca ggtttttcac gatgccggcg ttaacgttaa acaggtcgga
    89581 acgatccata cccggtttac gcgctacacc tgcagagata agaacgacat ctgcgccttc
    89641 cagcgccgga gtcgcatctt caccagaaaa acctttgatt ttcacagcag tagggatatg
    89701 gctcagatcg acagccacac cgggagtcac tggagcgata tcatacagag agagttctga
    89761 acctgaaggc agttgggttt ttaacagtag tgcaagcgcc tggccaatac cgccagcagc
    89821 gccgaggact gcgactttca tcctaaactc cttattatat tgataaacta agatatgttg
    89881 ctccgctgcc gcgaccttaa tccacaaaat tgccgtttac aatgaccaca tctcaagaat
    89941 gtgtagtcac gcaagtttag cgtttatgca tttaattgcc gtaatcagga acctaactta
    90001 cgtaattaac agtcgcttat gcattagcgc aacattccaa caggtggtga caatataccc
    90061 taccgttcag ccaaaacaac atcaatttga taacaattaa tttactttta agcagaattt
    90121 gcatgccgtg acgcaggcat gtttctcaat aacgaaattt gataaaatcc cgctctttca
    90181 taacattatt tcagccttct tcagggctga ctgtttgcat aaaaattcat ctgtatgcac
    90241 aataatgttg tatcaaccac catatcgggt gacttatgcg aagctcggct aagcaagaag
    90301 aactagttaa agcatttaaa gcattactta aagaagagaa atttagctcc cagggcgaaa
    90361 tcgtcgccgc gttgcaggag caaggctttg acaatattaa tcagtctaaa gtctcgcgga
    90421 tgttgaccaa gtttggtgct gtacgtacac gcaatgccaa aatggaaatg gtttactgcc
    90481 tgccagctga actgggtgta ccaaccacct ccagtccatt gaagaatctg gtgctggata
    90541 tcgactacaa cgatgcagtt gtcgtgattc ataccagccc tggcgcggcg cagttaattg
    90601 ctcgcctgct ggactcactg ggcaaagcag aaggtattct gggcaccatc gctggcgatg
    90661 acaccatctt taccacccct gctaacggtt tcaccgtcaa agacctgtac gaagcgattt
    90721 tagagctgtt cgaccaggag ctttaatctc tgccccgtcg tttctgacgg cggggaaaat
    90781 gttgcttatc cctctcaatc ccctgctttc ccctgcgatt catttaacga atagtgcgtt
    90841 ttaccgcgac atatcattca cacaatgaat acatacggta aaaaaagcac attatgcaaa
    90901 attcattatc tgattgaaaa aactggtatt tataataaat aaccgtgttt ttaatact
//
LOCUS       DAPHDX010000004        63160 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPHDX010000004 DAPHDX010000000
VERSION     DAPHDX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.004"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..902
                     /locus_tag="RH058_000290"
     CDS             30..902
                     /locus_tag="RH058_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV3135935.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(962..2260)
                     /gene="eno"
                     /locus_tag="RH058_000291"
     CDS             complement(962..2260)
                     /gene="eno"
                     /locus_tag="RH058_000291"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV3135936.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2348..3985)
                     /gene="pyrG"
                     /locus_tag="RH058_000292"
     CDS             complement(2348..3985)
                     /gene="pyrG"
                     /locus_tag="RH058_000292"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV3135937.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4213..5004)
                     /gene="mazG"
                     /locus_tag="RH058_000293"
     CDS             complement(4213..5004)
                     /gene="mazG"
                     /locus_tag="RH058_000293"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV3135938.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5075..5410)
                     /gene="mazF"
                     /locus_tag="RH058_000294"
     CDS             complement(5075..5410)
                     /gene="mazF"
                     /locus_tag="RH058_000294"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDV3135939.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5410..5658)
                     /gene="mazE"
                     /locus_tag="RH058_000295"
     CDS             complement(5410..5658)
                     /gene="mazE"
                     /locus_tag="RH058_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV3135940.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5736..7970)
                     /locus_tag="RH058_000296"
     CDS             complement(5736..7970)
                     /locus_tag="RH058_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV3135941.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8018..9319)
                     /gene="rlmD"
                     /locus_tag="RH058_000297"
     CDS             complement(8018..9319)
                     /gene="rlmD"
                     /locus_tag="RH058_000297"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV3135942.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPKLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9376..12132
                     /gene="barA"
                     /locus_tag="RH058_000298"
     CDS             9376..12132
                     /gene="barA"
                     /locus_tag="RH058_000298"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV3135943.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12364..13704)
                     /gene="gudD"
                     /locus_tag="RH058_000299"
     CDS             complement(12364..13704)
                     /gene="gudD"
                     /locus_tag="RH058_000299"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV3135944.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13725..15065)
                     /locus_tag="RH058_000300"
     CDS             complement(13725..15065)
                     /locus_tag="RH058_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV3135945.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15067..16419)
                     /gene="gudP"
                     /locus_tag="RH058_000301"
     CDS             complement(15067..16419)
                     /gene="gudP"
                     /locus_tag="RH058_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV3135946.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16854..17303)
                     /locus_tag="RH058_000302"
     CDS             complement(16854..17303)
                     /locus_tag="RH058_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV3135947.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17321..18103)
                     /gene="truC"
                     /locus_tag="RH058_000303"
     CDS             complement(17321..18103)
                     /gene="truC"
                     /locus_tag="RH058_000303"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV3135948.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18103..18432)
                     /locus_tag="RH058_000304"
     CDS             complement(18103..18432)
                     /locus_tag="RH058_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV3135949.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19054..19599)
                     /gene="syd"
                     /locus_tag="RH058_000305"
     CDS             complement(19054..19599)
                     /gene="syd"
                     /locus_tag="RH058_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV3135950.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19667..20515
                     /gene="queF"
                     /locus_tag="RH058_000306"
     CDS             19667..20515
                     /gene="queF"
                     /locus_tag="RH058_000306"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV3135951.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20627..21991
                     /gene="ppnN"
                     /locus_tag="RH058_000307"
                     /gene_synonym="ygdH"
     CDS             20627..21991
                     /gene="ppnN"
                     /locus_tag="RH058_000307"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV3135952.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22548..23837
                     /gene="sdaC"
                     /locus_tag="RH058_000308"
     CDS             22548..23837
                     /gene="sdaC"
                     /locus_tag="RH058_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV3135953.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23895..25262
                     /gene="sdaB"
                     /locus_tag="RH058_000309"
     CDS             23895..25262
                     /gene="sdaB"
                     /locus_tag="RH058_000309"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV3135954.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25374..26129
                     /gene="xni"
                     /locus_tag="RH058_000310"
     CDS             25374..26129
                     /gene="xni"
                     /locus_tag="RH058_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV3135955.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26184..27332)
                     /gene="fucO"
                     /locus_tag="RH058_000311"
     CDS             complement(26184..27332)
                     /gene="fucO"
                     /locus_tag="RH058_000311"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV3135956.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27360..28007)
                     /gene="fucA"
                     /locus_tag="RH058_000312"
     CDS             complement(27360..28007)
                     /gene="fucA"
                     /locus_tag="RH058_000312"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV3135957.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28554..29870
                     /gene="fucP"
                     /locus_tag="RH058_000313"
     CDS             28554..29870
                     /gene="fucP"
                     /locus_tag="RH058_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV3135958.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29903..31678
                     /gene="fucI"
                     /locus_tag="RH058_000314"
     CDS             29903..31678
                     /gene="fucI"
                     /locus_tag="RH058_000314"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV3135959.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31787..33205
                     /gene="fucK"
                     /locus_tag="RH058_000315"
     CDS             31787..33205
                     /gene="fucK"
                     /locus_tag="RH058_000315"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV3135960.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33207..33629
                     /gene="fucU"
                     /locus_tag="RH058_000316"
     CDS             33207..33629
                     /gene="fucU"
                     /locus_tag="RH058_000316"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV3135961.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33687..34418
                     /gene="fucR"
                     /locus_tag="RH058_000317"
     CDS             33687..34418
                     /gene="fucR"
                     /locus_tag="RH058_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV3135962.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34462..35562)
                     /gene="rlmM"
                     /locus_tag="RH058_000318"
     CDS             complement(34462..35562)
                     /gene="rlmM"
                     /locus_tag="RH058_000318"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV3135963.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35555..35950)
                     /locus_tag="RH058_000319"
     CDS             complement(35555..35950)
                     /locus_tag="RH058_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV3135964.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35969..36886)
                     /gene="gcvA"
                     /locus_tag="RH058_000320"
     CDS             complement(35969..36886)
                     /gene="gcvA"
                     /locus_tag="RH058_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV3135965.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(37237..37464)
                     /locus_tag="RH058_000321"
     CDS             complement(37237..37464)
                     /locus_tag="RH058_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV3135966.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            37656..38861
                     /gene="csdA"
                     /locus_tag="RH058_000322"
     CDS             37656..38861
                     /gene="csdA"
                     /locus_tag="RH058_000322"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV3135967.1"
                     /translation="MNVFNPAQFRAQFPALLDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38861..39304
                     /gene="csdE"
                     /locus_tag="RH058_000323"
     CDS             38861..39304
                     /gene="csdE"
                     /locus_tag="RH058_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV3135968.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(39355..40161)
                     /gene="tcdA"
                     /locus_tag="RH058_000324"
     CDS             complement(39355..40161)
                     /gene="tcdA"
                     /locus_tag="RH058_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV3135969.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(40238..41335)
                     /gene="mltA"
                     /locus_tag="RH058_000325"
     CDS             complement(40238..41335)
                     /gene="mltA"
                     /locus_tag="RH058_000325"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV3135970.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41544..41620
                     /locus_tag="RH058_000326"
     tRNA            41544..41620
                     /locus_tag="RH058_000326"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41578..41580,aa:Met,seq:cat)
     gene            complement(41695..42948)
                     /gene="amiC"
                     /locus_tag="RH058_000327"
     CDS             complement(41695..42948)
                     /gene="amiC"
                     /locus_tag="RH058_000327"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV3135971.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            43180..44511
                     /gene="argA"
                     /locus_tag="RH058_000328"
     CDS             43180..44511
                     /gene="argA"
                     /locus_tag="RH058_000328"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV3135972.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(44573..46399)
                     /gene="recD"
                     /locus_tag="RH058_000329"
     CDS             complement(44573..46399)
                     /gene="recD"
                     /locus_tag="RH058_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV3135973.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(46399..49941)
                     /gene="recB"
                     /locus_tag="RH058_000330"
     CDS             complement(46399..49941)
                     /gene="recB"
                     /locus_tag="RH058_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV3135974.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYNYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49934..52822)
                     /gene="ptrA"
                     /locus_tag="RH058_000331"
     CDS             complement(49934..52822)
                     /gene="ptrA"
                     /locus_tag="RH058_000331"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV3135975.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52998..56366)
                     /gene="recC"
                     /locus_tag="RH058_000332"
     CDS             complement(52998..56366)
                     /gene="recC"
                     /locus_tag="RH058_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV3135976.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPTTGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(56379..56702)
                     /locus_tag="RH058_000333"
     CDS             complement(56379..56702)
                     /locus_tag="RH058_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV3135977.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56687..57094)
                     /locus_tag="RH058_000334"
     CDS             complement(56687..57094)
                     /locus_tag="RH058_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV3135978.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(57091..57654)
                     /locus_tag="RH058_000335"
     CDS             complement(57091..57654)
                     /locus_tag="RH058_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV3135979.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(57645..58115)
                     /locus_tag="RH058_000336"
     CDS             complement(57645..58115)
                     /locus_tag="RH058_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV3135980.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(58300..59094)
                     /gene="thyA"
                     /locus_tag="RH058_000337"
     CDS             complement(58300..59094)
                     /gene="thyA"
                     /locus_tag="RH058_000337"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV3135981.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(59101..59976)
                     /gene="lgt"
                     /locus_tag="RH058_000338"
     CDS             complement(59101..59976)
                     /gene="lgt"
                     /locus_tag="RH058_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV3135982.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(60127..62373)
                     /gene="ptsP"
                     /locus_tag="RH058_000339"
     CDS             complement(60127..62373)
                     /gene="ptsP"
                     /locus_tag="RH058_000339"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3135983.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(62386..62916)
                     /gene="rppH"
                     /locus_tag="RH058_000340"
     CDS             complement(62386..62916)
                     /gene="rppH"
                     /locus_tag="RH058_000340"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV3135984.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT        15306 a        17037 c        15435 g        15382 t
ORIGIN      
        1 atttatccag atttaatgag aaatttataa tgcgctattt catactaatg ttcactttcg
       61 tgtgttcctt tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg
      121 atcttacgag tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta
      181 ttttcaataa tacccaagtg caaattgctg tattaattgt tcccacaacc aaagacgaga
      241 caattgaaca atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta
      301 atgatgggat actgatcatt gttgcctggt cagatcgtac tgtccgcatc caggtaggtt
      361 atgggctgga agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga
      421 tacccgcatt taaacaacaa aaatttgctc agggattaga gctagctata aacgctttga
      481 ataaccaact cacttcacaa caccaatatc cgactaatcc ttcagaaagt gaatcagcgt
      541 cttccagtga tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt
      601 tctggttttt tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctatattt
      661 cagcaatcta tcttttagat ttattcctgt tctccgataa aattttttcc attgctgtat
      721 tttccttctt ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga
      781 aaagagcatc aggtaaaagt tatcattcag acaacagcgg ttccggtgga ggctcaagtg
      841 gcggcggatt tagcggcggt ggcggttctt ctggaggtgg cggcgcatcc ggccgctggt
      901 aactcaacca taaaaaatgc cagcccggag gctggcattt ttaaatcaga taaagtcagt
      961 cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg cccagagctt
     1021 cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc atagaaccag
     1081 ttttgatctg gcctgcagca gtaccaacag ccaggtcagc gatggtagcg tcttcagttt
     1141 cgccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc ttgattgcag
     1201 ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag ttagcgatac
     1261 ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg tcaccaacca
     1321 gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag tcagattcgt
     1381 ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc aggaagtgag
     1441 tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat ttaccatctt
     1501 tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc agttcataac
     1561 cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa cccaggttcg
     1621 gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc agaacttttg
     1681 ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt ttcgcgccaa
     1741 ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca ccaccgttga
     1801 tgatgttcat catcggaacc ggcatagagt atttgcccgg agtaccgttc agttcagcga
     1861 tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta gccagagata
     1921 cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc aggtcgatca
     1981 tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc tgagcgatcg
     2041 ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg gatttgtcgc
     2101 catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga gcagctgcca
     2161 taccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta ccacgggagt
     2221 cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc tcaagtcact
     2281 agttaaactg aaactccaga caaacaacgc gtacccaggg tacgcgttgc cgttctaact
     2341 tttttactta cttcgcctga cgtttctgga actcgctggc ggctttcaca aagcctgcaa
     2401 acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg caagccacga
     2461 accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg gaacggcccg
     2521 caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact tcgtaacggt
     2581 gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg gcgaaccagg ctatcgtcaa
     2641 ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc ttctcgctac
     2701 gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca accgggtact
     2761 tacagtctgg cacaaattcc gtagagttgg cgttctccat gttggcaaca tggcgagcgt
     2821 aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata ttgttctcac
     2881 gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccaaaa ccgccaggta
     2941 ctaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca tcttgtgaat
     3001 cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc agtgcttcga
     3061 tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg atggtgactt
     3121 cacttaccgg gttcgcttct tcgaagataa cctgttccca ttcggacaga ttcgcttccg
     3181 ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat ttcaacaggc
     3241 ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt tccggaacat
     3301 tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac agcgcgatct gaacgacaaa
     3361 tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc tgagtcggtt
     3421 tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc ataaacagag
     3481 tgtgctcacg gccaatttca acagccatct ggcgaatcgc ttcgaggaac ggcaaggatt
     3541 cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct tcgccacctt
     3601 ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg gttgcgccga
     3661 ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc gtggtgaagt
     3721 tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg tccaggtcgg
     3781 tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc atagtacctg
     3841 gatcgacgtt gatgtacgga tccagtttca tgatggtcac attgaggcca cgggcttcaa
     3901 gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg accccgccgg
     3961 tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa aagacgatgg
     4021 aataaccagg acgggaaagc agtatacccg aacatgacct gtgccacaaa ctttcattat
     4081 ccctcctctt cgccagcgca ctattgaaat caggagtgag aaaatagccc ctttggggta
     4141 aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat ggcgcttgac
     4201 cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga cttcttccat
     4261 tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac gctccacttc
     4321 gcgaaaacga cgctcgaatt tttcgttcgc tttttgcaat gcgatttctg ctttcgtccc
     4381 taaatggcga gccagattaa ccgtggcaaa cagcaggtcc cccatttcct cctccagttt
     4441 agcctggtcg acaacagcct gccgcgcttc gtacatcacc tcgtcgatct cttcgtagac
     4501 tttatcgact accggaccaa gcgtcgtcca atcgaagcca acgttggcgc aacgtttctg
     4561 gattttttgc gcacgcatta aagccggtaa actacgagga atatcgtcca gcgccgaatg
     4621 ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa gcacttcact
     4681 actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta atttatcgct
     4741 aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca tttgcgcgta
     4801 aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa aatcttcacg
     4861 ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa tggtggcaaa
     4921 tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct gcataatagt
     4981 gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct gtcaggtgga
     5041 aacctgtgac cagaatagaa gtgagttagt aacactaccc aatcagtacg ttaattttgg
     5101 ctttaatgag ttgtaattcc tctggggcaa ctgttccttt cttcgttgct cctcttgccc
     5161 gccaggcgat actttttacc tgatcagcta acgctacgcc atcacgttcc tgaccggata
     5221 aaacaacttc gaacggatat ccttttgatt gcgttgtata aggaacacac agacacatac
     5281 ctgttttgtt gttgtacatg aaaggactca ggacaacagc tggacgatgt ccagcttgct
     5341 cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc gcccatatcg ggtacgtatc
     5401 ggcttaccat taccagactt ccttatcttt cggctctccc cagtcgatat tctcgtggag
     5461 gttttccggc gtgatgtcgt tgaccagttc agcaagcgta aatacgggct ctttacgcac
     5521 tggctcaata attaatttgc catccaccag gtcaatcttc acttcatcat caatattcag
     5581 attgagcgcc tgcattaacg tagccgggat ccgcaccgcc ggtgaatttc cccaacgctt
     5641 tacgctactg tggatcataa ccctttcctc aaaccgctat catatgtaga tacagtatat
     5701 atcaatctac attgtagata cgagcaaatt tcggcctaac tcccgtgcaa ccgacgcgcg
     5761 tcgataacat ccggcacctg gttgagttta cccagcacgc gccccagcac ttgcaggttg
     5821 taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt tggtgtcgct acggctggca
     5881 acgccaagca cgttcacctt ctcgttggcg agaatggtcg tgatatcacg taacaaccca
     5941 ctacgatcat tagccaccac gcggaccacc agcgaatatc cggcggagta gctctcaccc
     6001 cataccgcgt caacaatgcg ttctggcgca tgggagcgca gttccgccag ttgttcgcaa
     6061 tcggcgcggt gtactgaaat accgcgcccc tgggtaatga agccgacaat ctcatctcca
     6121 ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt tgccaacacc ttcgaccacc
     6181 acgcgaccgt tatctttact gcggttttgc ggcgtgtagc ttttttgctg aagttgcttc
     6241 agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa attgcgattg caggaagttc
     6301 accatctgat tgagacggat atccccgcca ccaatcgccg ccagcaactc gtcgacatca
     6361 ttaaagttgt aacgcggcag cagatgtttt tctgcttctt tcaggctgat ccccagatgt
     6421 tccagctcgt cgtcaaggat ttgccgccca gccagaatgt ttttgtcacg gtcctgttta
     6481 cggaaccagg cgtgaatttt cgaacgccca cggctggttg tgacgtaacc gaggtttggg
     6541 tttaaccagt cacggctggg gttcggctgt ttctgggtga taatttcaat ctggtcgccc
     6601 atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa tttttgcccc gatgcagcgg
     6661 tgtccgacat cactgtggat gtggtaagcg aagtccagcg gcgttgatcc cgcaggcaaa
     6721 tcaacgacat cacctttcgg cgtaaagaca tacacccggt cgtcaaagac ctggctgcgt
     6781 acttcgtcga gcatttcgcc ggaatcagcc atctcttcct gccacgcaat cagtttacgc
     6841 agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc cagctgccgc gccctcttta
     6901 tatttccagt gcgcagcaac acccaactct gcatcttcat gcatctgttt ggtgcggatt
     6961 tggatctcaa cggttttgcc acctggcccc agaaccacgg tatgaataga ctgataaccg
     7021 tttggtttcg ggttagcgac ataatcgtca aactcatccg gcaggtggcg atagtgagtg
     7081 tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct cggcgacaat acgtaccgca
     7141 cgcacatcaa acagctcatc gaaggcgagg ttctttttct gcattttgcg ccagatactg
     7201 tagatgtgtt tcggacgacc atacacttca gctttaacgc cttcagcttt catctcagcg
     7261 cgcagatggc cgacaaactc ttcgatatag tgttcgcggt cgagacggcg ttcatgcagc
     7321 agttttgcga tgcgtttgta ttcggtcggg tggagataac ggaagcagta atcttccagc
     7381 tcccatttca gttgcccaat cccaagacgg ttcgccaacg gcgcgtagat attggtgcac
     7441 tcttttgccg ccagtacgcg ttcatcttcc ggcgcatctt ttacttcgcg cagatgagca
     7501 atacgctccg ccagtttgat gaccacgcag cgaaaatcat cgaccatcgc caataacatc
     7561 cggcgaacgt tatcgacctg ttcggaggaa acagaatcag tgtgcgtcgc tttcagctgg
     7621 cggatcgccg ccatatcacg cacgccgtga ataaggttaa cgaccgactt accgacgctc
     7681 tcacgcagca catcttcgct gactacgttg gcatcagcca gagggaacag cagcgccgcc
     7741 cgcagcgtgt caatgtccat acttaatgtc gagaggatct ccaccatctc aacaccacgc
     7801 cacaataaca gactggcatc cggatgcccc tgcgtctgtt gcagacaata cgcccaggtt
     7861 tcggctaagc actcacacga cttctggctg gtaataccca gacttgcgat ccatttttcc
     7921 ggatcaaatt caccagcctt attgatatgt gcacttctta ccgcaaccat tgtcctctcc
     7981 tttagggacc agacctgccg aaatcggcaa atcgtaacta tttaacgcgc gagaaaagta
     8041 ccatcgattc cagatgtccc gtgtgtggga acatatccag catcgccagt cgcgcaatgg
     8101 tatatcctgc ttttaataac gcttcgctat cccgagccag cgttgcaggg ttacaggata
     8161 cataaactat acgaataggt tccagtttta taatttgctg cataacacct gcggcacctg
     8221 ctcgcgccgg gtccagcaac actttatcga agccgttttt cgcccacggc tgctttgtga
     8281 catcttcttc aagattttcg tgataaaacg tcacattctg taaaccatta agacgcgcat
     8341 tctgctggcc tttttccacc agcgccggaa caccttctac accgaccaca ctggctgctt
     8401 gtttcgccaa tggcagtgta aagttgccca taccgcagaa cagatccagt acgcgatctt
     8461 caggttgcac atccagccat tccaacgcac gcgctaccat tttttggttc acacccgcat
     8521 tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa cccgtttgag tcataccagg
     8581 gcatctcacc agagacggtt tcgagtatct cactatcggg ggcgagatac agatccaggc
     8641 cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc cgaacttagc ggtgcggtat
     8701 ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag ttcaacatga ccaagatggc
     8761 gcataacttg taagctgccc aggcatgccc tgactttggg cagcaatgct tcaagtttgg
     8821 gcactaaaat ggggcattgt ttaacgtcga caatgtcact ggagcccgct ttgcgaaagc
     8881 ccatctgaag ttgctgtgtt ttcggtaagt agttcaaact taaacgcgcg cgacggcgat
     8941 agccccaggg aacatcggcg atcacttcag agacttcgtg tttcattaat cgggcgagtg
     9001 ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg ttgttgctga cagccaccgc
     9061 atacgccaaa atgagggcag cgtggcgttt cgcgttccgg gctatcgctt aaccggcgca
     9121 cgactttagc gcgggcgtac tgttttttat cttcagtaac agtaacttcc gcgttttcct
     9181 gtggcagtaa tccaggaata aataacgctt tgccgttatg tcgcgccacg ccctgaccaa
     9241 aagagtcgag gtcgttgact gaaacggtta tgatctgacg cgtcgtcgtg cgtcgttttg
     9301 cagagtagaa ttgcgccatt ggcgagactt tctcaattta acagtgtgac cttaattgtc
     9361 ccataacgga actccatgac caactacagc ctgcgcgcac gcatgatgat tctgatcctg
     9421 gcaccgaccg tccttattgg tttattgctg agtatctttt tcgtcgtgca tcgctataac
     9481 gacttgcagc gtcaactgga agatgccggt gccagcatta ttgagccgct tgcagtttct
     9541 actgaatatg gcatgagcct gcaaaatcgc gaatctatcg gtcagttaat aagcgtactg
     9601 catcgtcgcc attccgatat tgttcgcgcg atttcggttt atgatgaaaa taaccgactc
     9661 tttgtcacct ccaattttca tcttgatccc tcatcaatgc agctcggcag caacgtgccg
     9721 tttcctcgcc agctcactgt cactcgtgac ggcgatatta tgatcctccg cacgccgatt
     9781 atttctgaga gttactcccc cgacgaatcg cccagtagcg atgccaaaaa tagtcaaaat
     9841 atgttgggat atattgcgct tgagctggat cttaaatcgg ttcgcttgca gcaatataaa
     9901 gagatcttta tttccagcgt gatgatgctg ttttgtatcg gtattgcgct tatttttggc
     9961 tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca tggtgaatac cgtcgaccgc
    10021 atccgtcgcg ggcaactcga cagccgggtg gaaggattta tgctcggcga gctggatatg
    10081 ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg cttatcacga agagatgcag
    10141 cacaatatcg accaggcgac gtccgatctg cgtgaaacgc tggagcagat ggaaattcag
    10201 aacgttgagt tggatctggc gaaaaagcgc gcccaggaag cggcgcgtat taaatccgag
    10261 tttctggcaa atatgtcaca cgagctgcgt acaccactga atggtgttat tggctttacc
    10321 cgcctgacgc tgaaaacaga attaacacca acgcagcgcg atcacctgaa tacgattgaa
    10381 cgttcggcaa ataatttgct ggcaattatt aatgatgttc tcgacttctc gaaactggaa
    10441 gcaggtaagc tgattctgga aagtattcca ttcccactac gcagcacgct ggatgaagtc
    10501 gttactctgc tggcacattc ttctcacgat aaagggttgg aactgacgct caatattaaa
    10561 agcgacgtgc ctgataacgt gatcggcgac ccgctgcgat tacagcaaat catcactaac
    10621 ctggtgggga atgcaattaa attcaccgag aatggcaaca ttgatattct ggtagaaaaa
    10681 cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga ttcgggatac cggcattggt
    10741 attcctgaac gcgatcaatc gcgcttattc caggccttcc gacaggctga tgccagtatt
    10801 tcccgccgtc atggtggcac cgggctgggg ctggtgatta cacaaaaact ggttaatgaa
    10861 atgggcggcg atatttcgtt ccatagccag ccgaatcgcg gttcaacttt ctggttccac
    10921 attaatctcg atctgaaccc gaacattatt atcgaagggc catccaccca gtgcctcgca
    10981 ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag cgcaatgcac gctggatatt
    11041 ttaagtgaaa cgccgctgga agtggtttat agcccaacgt tctccgcgct gcctcccgcg
    11101 cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc gcgagccgct aacaatgcaa
    11161 catgagcgat tagcgaaagc ggtatcgatg accgatttcc tgatgctggc acttccttgc
    11221 catgcacaag tcaatgctga aaaactcaag caagatggta tcggcgcgtg tctgctgaaa
    11281 ccattaacac ctacacgcct gttgcctgcc ctgacggaat tttgtcatca caaacaaaac
    11341 acgcttttgc ctgtaaccga tgaaagtaag ctggcaatga cagtcatggc ggttgatgac
    11401 aaccccgcta acctgaaact tatcggcgca ttgctggaag atatggtgca acatgtggaa
    11461 ctttgcgata gcgggcatca ggcggttgaa cgggcgaaac agatgccgtt cgatttgatc
    11521 ttaatggata ttcaaatgcc tgacatggat ggcattcggg cctgcgagct catccaccag
    11581 ctcccccatc agcaacaaac gccggttatc gcggtaacgg cgcatgcaat ggccgggcaa
    11641 aaagagaagc tgcttggcgc agggatgagc gattatctgg cgaaaccgat tgaagaagag
    11701 cgattgcata atttgttgtt gcgctacaag cctggcagcg gtatttcctc tcgcgtcgtg
    11761 acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga ccctcgactg gcaactggca
    11821 ctacgccagg cagcaggaaa aaccgattta gcgcgcgata tgctgcaaat gttactcgat
    11881 ttcctgcctg aagttcgcaa caaagttgag gaacagctgg ttggagaaaa cccggaaggc
    11941 ctggttgatt tgattcataa actgcatggc agttgcggct atagcggtgt gccgcgtatg
    12001 aagaatctct gccaactgat cgaacaacag ctacgtagcg gtactaaaga agaagatttg
    12061 gagccggagc tgctggaact gttggacgag atggataatg tagcgcgcga agccagcaaa
    12121 attctcgggt aacggacgtc ttgtcgagta gcaatcggct ttttatgacg ctggtttcag
    12181 attggtgaca aagtgcctgt gtttatgccg gatgcggcgt taacgcctta tccggcctac
    12241 aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac ctgatacata gcacaacagg
    12301 ctattttgcg tttagcatca gtctcaagcc ggctccagat agagccggtt ttggttttct
    12361 gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc cagccaggaa tcagatactg
    12421 cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc tgatacagct catgggcttt
    12481 catcacttga tccatatcga tttctacacc cagccccggt ttttctggca cctgtaccag
    12541 cccgcctttg atctcaaacg gttctttggt caggcgctga ttaccttcct gccagatcca
    12601 gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg gcaacatggg taaacatcgc
    12661 cagggaaata tcgaagtggt tgtttgagtg tgaaccccag gtcaggccaa attcatggca
    12721 catttgcgcc acacgtaccg aaccttgcat cgtccagaaa tgcggatccg ccagcgggat
    12781 atcaacggat tgcagggaga gcgtatggcc catctgccgc cagtcggtgg cgatcatatt
    12841 ggttgcggtc ggcaggccag ttgcgcgacg gaactctgcc atcacttcac gcccggagaa
    12901 accttgctcc gcaccacacg gatcttctgc ataagccagc gaccctttca ggtatttacc
    12961 aattttaatc gcttcgttca gcgaccaggc accgttagga tcgagcgtaa tacgcgcctg
    13021 cgggaagcgt tgcgccagtg ccacaataga ctcggcctct tcttccccgg ccagtacacc
    13081 gcctttcagt ttgaaatcgt tgaagccata tttttcatac gccgcttccg ccaggcgcac
    13141 caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga taccagtcgc atgagtcatc
    13201 cggctggctt tgatacggca gcggtgtggc tttgcgatca ccgacgaaga acagataacc
    13261 gagcatttcg acttcgctac gctgttgacc atcgcccagc agggatgcca cgtttacccc
    13321 cagatgctgc cccagcagat ccagcattgc cgcttctatc ccggtaacta catgaatagt
    13381 ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccagca tcacgatcgg caaaagtatt
    13441 acgcaccagc gtcagaacgt ttttgtattc acccagcgtt ttacctacca ccagcggaat
    13501 cgcatcttcc agcgttttac ggattttctc gccgccggga atttccccta cgccagtgtg
    13561 accagaatta tctttgataa tcacaatatt acgcgtaaag aacggtgcgt gtgcaccact
    13621 cagattcatc agcatactgt catgacccgc caccgggatg acctgcattt cagtaacaac
    13681 aggcgtcgta aattgagaac tcatatttat gtcctttttc agaatcaatg acggccgaaa
    13741 acgggacgtt tacggtcaaa ggtccagccg gggatcaggt actgcatcgg acctgcgtcg
    13801 ttacgcgcac cgacaggcag acgtttatag gcctcatgtg ccttttgtac ctgttcccag
    13861 tccagttcca cgcccagacc gggcgcatca ggaacggcaa tttttccgtt tttaatctcc
    13921 agcggatttt tggtcaggcg acaatcgccc tcctgccaaa tccagtgggt atcgatagcg
    13981 gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca tcgccagaga gatatcgaaa
    14041 tggttattag aatggcagcc ccaggtcagc ccccagtcgt cgcaaagctg cgccacacgg
    14101 actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg gaatatctac cgcattgagc
    14161 atcaccgcat gacccatttc gcgccagttg gtggcgatca tgttagtcgc gacgggcaag
    14221 ccggtcgccc gccgaaactc cgccataact tcacgaccgg agaaaccctg ttctgcgccg
    14281 catggatctt cggcataggt aagaacatca ttcagccctt tgcatagaga aatggcttca
    14341 tcaagtagcc aggcaccgtt gggatcaacg gtaatccgcg catcagggaa gcgtttcttc
    14401 aatgcacgaa cagtgtcgat ttcttgctcg ccaggtaaca ccccgccctt aagtttgaaa
    14461 tctttaaagc catagcgatc ctgtgaggct tccgccagac gcacaacggc ttcgctgttc
    14521 atcgctttct gatggcgcaa ctgataccac tcatggttgc ccggcgtatt ttccagataa
    14581 ggaagatcgg ttttggtccg atcaccgata taaaacagat aaccgaggac ggtaatagtc
    14641 tcgcgttgct tgcctggccc caacagttcg cagaccggaa cattcagcgc cttacctagc
    14701 aggtcaagca aggcggcttc cagcgccgcc acggcattaa cgcgcaattc aaaagtccag
    14761 gcacctttgc cgaaggtatc aaaatcggct gcctgattac ctttatgcac ctgctgaacc
    14821 actttattca ggcgcgcaac ttcctggccc agaaccatcg gaatagcatc gaccagtgtt
    14881 tgataaatca cctctccgcc cggcgcttca ccaatgccgg tatgcccggc gttatcggtg
    14941 agtaccacaa tattgcgggt gaaatatgcg ttatgtgccc caccaatatt aagcaacatg
    15001 ctatcgtgcc cggcgaccgg aatgactttc atatcagtaa taacaggact ggattgtgtc
    15061 gtcatcttat tgccccgcga caggtttcag ctcgatacgc ttgatatcgc ccaccagcac
    15121 caggtagctc agtaccgcga ttaaggcatg aacaccaaca taaatcagcg ccccattaaa
    15181 ggagccagtc gtgccaacga tataaccaat tgcgattggc gtgacgatgc cagaaatgtt
    15241 accgaacatg ttgaacaggc caccggaaag accgctgatc tcttttggcg cggtatctgc
    15301 cattactgcc caacccagcg ccccgatgcc cttaccgaag aaggccagcg ccataaagcc
    15361 gatgatcatc cactcaacgt tgacgtagtt gcagaacacc atcaccatcg acagcaacat
    15421 gcccattacg atcggtgttt tacgcgcaat gttcagcgat cccgtgcggc gcatcagcca
    15481 gtcggaaata atcccaccca gcacaccgcc gataaaaccg caaaccgccg gaacggaagc
    15541 cacaaagccc gctttcagaa tcgacatccc acgcgcctgc accagataaa ccggaaacca
    15601 ggtaatgaaa aagtaagtca gggcgttgat acagtactga ccgatataaa cgccgatcat
    15661 catccgtgac ccgagcagtt gtttgatctg cccccacttc acgctgaacg gcactttaac
    15721 tttggtgttt tgctgatcca tattgatcag cgcgccaccc gcggcgatat actccagctc
    15781 tttctgattt acccccggat gttgatttgg ctcgtggatg actttcaacc agataaagct
    15841 gatgacaatc cccagaccgc ccataaagaa gaagacgtgt gaccagccca cttcatgcgt
    15901 cagccagccc ataatcggcg cgaagatcac cgttgcgaag tattgagcgg agttaaaaat
    15961 cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc gcaacaatgc gactgttgcc
    16021 ggggaaagat ggcgcttcag caagcccgac caggaagcgc agagtaaaca gggcaacgat
    16081 aatgccgaat ccactaaaga tatcgacgaa gccttgcagc aaggtaaaca tcgaccagat
    16141 aaagatcgac cagaagtaga cgcgttttga accaaaacgg tccagcaacc agccaccagg
    16201 gatctgcccg ataacataag cccatgagaa ggcagagaac acatagccca ttcccacggg
    16261 atcaaggccg atatctttgg ccatttccga accggcaata gaaagcgtag cgcggtcgcc
    16321 gtagttgaag gatgtgacga taaacaacat caccactatc cagtaacgag cattcgtgcg
    16381 tttttccaca ctgctcgcag cctgacttaa agaactcatt gttgcactcc tgaaaattcg
    16441 cgttagccac gctcactctg gactgcgaca tagccaggaa atcagaggtg acgtagggtg
    16501 ttttttgccg tttttatagg tcgttcgccg aatacggcgc gtgtttatat ctggcaatag
    16561 cagtataaaa agcgcgccat agcggctcac cgtgcaacaa cacaacatta atgcgttcaa
    16621 tgaggcccga ttttggcatt agccctggac ggtggaatcc acttcacgga aatgaaaaca
    16681 agaacaagaa aggaagggtt aaaacgaaga aataaagaag agtatgaaat ggatcgcttg
    16741 actccaggca aacgccagta aaaatccgcg ctatgaagca gtttttactg gcatttgcct
    16801 gaaaagattc gattcagcac cgctaaaacg acatttaccg ctcgctgaac atatcaggac
    16861 aacagcgtgc cccactgttc gacccacgga tttgattccg tttccggttc cgggttctca
    16921 ctggcgtcaa tcaacagtat ttcgccaacc cgctgagcgc tctgttcctg caacaaggca
    16981 tcgaactgtt tgccgccatt gcagaaattt acataactgc tgtcgccgag cgcaatcacg
    17041 ccataacgca gattcggctg gaagcccaga ctatctttga ttccctgaaa gagcggcaca
    17101 atgctatcag gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac atacttatcc
    17161 tgataaggca gccagtcgct taattcagga tcttcaaata ccgttgcttt gtggccctgt
    17221 gcggtcagaa ttgcttctgc ttcttccgcc actaacagcg aatttccgta catggtgccg
    17281 acaaaaatac caatttccgc cataccgttt tctccctgga ttaggaactt atctctccat
    17341 cctgacccga tggcgcactg aactcaaccc tttcattttc aggaagcaga ccgcgccagc
    17401 caaattgtga taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct
    17461 cgccagtaaa aggatgcgtc aatgacaact gactggcatg tagcattaac cgctggaggc
    17521 caaaatgctc agcaccgctg cgattctggc gtaaatcgcc atgtttgcta tcgccaataa
    17581 tcggatgacg caaatgggca agatgtcggc gaagctgatg tttgcgtccg gttttcggct
    17641 ccagttccac caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta
    17701 cggtcgccag accgcgataa tgcgtcactg ctggctgcga gcctttatct tcgcgggcaa
    17761 atttatcagc gattttgtcc agttcttcca ccagtggata atccagaact gcttcttcca
    17821 tcaaccagcc gcgcacaatc gcatggtaac gtttctggat ttggtgctgt tcaaactgtt
    17881 gtgccagcag ccgtccggcc tcgctggata atcccatcaa caacacacca gaagtgggtc
    17941 ggtccagacg atgagcggta aaaacgtgct ggccaatctg atcacgcacg gtttgcatga
    18001 ccactacttt ctcgtcgcga tccagccagc tgcggtgaac cagccagccg gagggtttat
    18061 ttaccgcaac cagccattca tcctgataga gtatttccag cattagctcg catcatccgc
    18121 aaaaagagca tccagttttt ccaactcagc cagaataagc gcgcgttgcg gatggtccgt
    18181 cgccagcgcc atttcataat agggtgcaac agcaaaagcg cccggtaacg gctggttgtt
    18241 atttaacaaa tcgtgcattc gcgggatcag cacccactgc aaccactcca gtggttccat
    18301 ggtgtccata aagaacggtt gggtactatt aaattgatgc ggctggggtt catcgtttcg
    18361 ccagtgctga tgttcacgca gtaacgcttc aagcgcctgc aactggagac gaacgcggtc
    18421 atgagtggtc ataaagcaac ctcaataaga aaaactgccg cgaagaatag caggaataaa
    18481 aaaagggagc actgtattca cagcgctccc ggttcgtttc gcagcattcc agctactttt
    18541 gttgctccct gctcatcctt gacaactttt cctctggcct tgcggccaat cgttcatcct
    18601 gaactattgc ttcctgctca caccaccccg atgtgatact tcatcctgaa gtgtccctgg
    18661 ccttcctgac ccaccgaatc atcctgaccg gttctcattc tccatcctgg aggtgtcctt
    18721 taacgcgtcc tgcgtcatcc tcttcgcttc atccagaaac ctttccctga aacaccatcc
    18781 tggtgtgtcc tgcagaagtg tcatcatcct gatgttcact tcgttgtctg actccctgtc
    18841 gacgaagata gaatcgtctt tttcgcgaag tcttacaagg cgcttacaga caatgcctta
    18901 aggaaaattt catatgaaag ttaagaatta acaatcaaca ttttgatttt aaatgaaaat
    18961 aacgattctt atctttttaa gtattcgctt atttcctggc gatctcctac agagtctgta
    19021 agagatctct cacaaccaga tggggtattt ggattacaga agaggcttaa gttgattaag
    19081 gaattccgca agatttgagg ctaaatgcgt ccgtttacgt gttcccagcg tctctttgca
    19141 cacttcaccg gagagattac acaccgagat gacctctaac tcttcttcca gcgtcgcgat
    19201 aaatagcgtg ggcggcagtt ttagcctttt ctgagtcacc agatgaccaa tcagattttc
    19261 ctgcacccga cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc
    19321 aaactgcgcg tgcatatccc cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg
    19381 tatcacaata tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg taaacggctg
    19441 gggttgccag tatacggcat cttcggtagt tgaaataatg cacggtgaag gaacaccgta
    19501 cagttcctca cttaacggcc agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc
    19561 agtaaaatct ttcagggctt gtgcggtcaa atcgtccact aatttctctc ttcacttaaa
    19621 ccagatacac ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt cttcttatgc
    19681 aaaccatcag gcacttgcgg gcctgactct tggaaaatca accgattacc gggataccta
    19741 tgacgccagc ctactgcaag gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa
    19801 agcggataac ctgccttttc acggtacgga tatctggacg ctgtatgaac tttcctggct
    19861 gaatgcgaaa ggtttgccgc aggttgccgt cggtcacgtt gaacttgatt acaccagcgc
    19921 aaatctgatt gagtcgaaga gttttaagct ctatctcaac agttttaacc agacgcgttt
    19981 taataactgg gatgaggtgc gccagacgct ggagcgcgac ttaagcactt gcgcccaggg
    20041 taaggttagc gtggcgttat atcgtcttga tgaactggaa ggccagccga taggtcattt
    20101 taatggcact tgcattgatg accaggatat cactatcgat aactatgaat tcactactga
    20161 ctatctggag aatgccacct gtggtgaaaa agtagtggaa gagacgcttg tcagccacct
    20221 gctgaaatca aactgcctga tcacccatca accagattgg ggttcgatcc aaattcagta
    20281 tcgtggacgc caaattgaca gagaaaaact gctgcgttac ctggtctcat tccgtcatca
    20341 caacgagttc cacgaacagt gcgtggaacg catctttaat gacctgttac gcttctgcca
    20401 gccagaaaaa ttgagcgttt acgcacgtta tacccgtcgt ggcggtctgg acattaaccc
    20461 gtggcgcagt aatagcgatt ttgtcccatc gaccacaaga ctggttcggc aataaatttt
    20521 ttctcaattt tgcgtgctgg attcacgcag aaggttgtga aaggtcatca ggcagggcta
    20581 ttgtaatcaa agggaatgac gatattcgtc ccataaggag tttttcttga ttacacatat
    20641 tagcccgctt ggctccatgg atatgttgtc gcagctggaa gtggatatgc ttaaacgcac
    20701 cgccagcagc gacctctatc aactgtttcg caactgttca cttgccgtac tgaactccgg
    20761 tagtttgacc gataacagca aagaattgct gtctcgtttt gaaaatttcg atattaacgt
    20821 cctgcgccgt gaacgcggcg taaagctgga actgattaat cccccggaag aggcttttgt
    20881 cgatgggcga attattcgcg ctttgcaggc caacttgttc gcggttctgc gagacattct
    20941 cttcgtttac gggcaaatcc ataataccgt tcgttttccc aacctgaatc tcgacaactc
    21001 cgtccacatc actaacctgg tcttttccat cttgcgtaac gctcgcgcgc tgcatgtggg
    21061 tgaagcgcca aatatggtgg tctgctgggg cggtcactca attaacgaaa atgagtattt
    21121 gtatgcccgt cgcgtcggaa accagctggg cctgcgtgag ctgaatatct gcaccggctg
    21181 tggtccggga gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg cgcagcagcg
    21241 ttacaaagac agtcgtttta ttggtatgac agagccgtcg attatcgccg ctgaaccgcc
    21301 taacccgctg gtcaacgaat tgatcatcat gccggatatc gaaaaacgtc tggaagcgtt
    21361 tgtccgtatc gctcacggca tcattatctt ccctggcggt gtgggtacgg cagaagagtt
    21421 gctgtatttg ctgggaattt taatgaaccc ggccaacaaa gatcaggttt taccattgat
    21481 cctcaccggc ccgaaagaga gcgccgacta cttccgcgta ctggacgagt ttgtcgtaca
    21541 tacgctgggt gaaaacgcgc gccgccatta ccgcatcatc attgatgacg ccgctgaagt
    21601 cgctcgtcag atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgtg atacaggcga
    21661 tgcctacagc tttaactggt caatgcgcat tgcgccagat ttgcaaatgc cgtttgagcc
    21721 gtctcacgag aatatggcta atctgaagct ttacccggat caacctgttg aagtgctggc
    21781 tgccgacctg cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag aagtcggtat
    21841 tcgcgccatt gaagagtttg gtccttacaa aatcaacggc gataaagaga ttatgcgtcg
    21901 tatggacgac ctgctacagg gttttgttgc ccagcatcgt atgaagttgc caggctcagc
    21961 ctacatccct tgctacgaaa tctgcacgta atctcccctc ccccggtgac gtttgttgcc
    22021 gggttttctc gtttttggtc acttactcat caactcattt catttgttat atgaatgttt
    22081 cttaccaccc tcacgcgaca aatatcatca cagttaatat gtcatacaat ttatgttgca
    22141 atgcaaacgt ttccctattt tcataaaccg tattttatcg ctatgaaaaa gaaattatcg
    22201 ccatgattaa ctaaaagtat tgattttttc agttcaacct acatatattg cgcgcccagg
    22261 aagaagtcag atgtcgttta atgggcaaat attgccctta aattctcttt tacttttgat
    22321 ttacagagta aagcgttggg ataatctatc ttccaagtag attattgtat ttgggatcaa
    22381 gatcactgat agatacataa cttgtgtgta tctttccgcc ctcaaattat tacggcggta
    22441 aatgattaag ccatcgccga tagacagatt tcatttttac ggtcaggcac cttcccgggc
    22501 tgaactggct aaaagctgaa ttatttgcat tcctccagga gaaatagatg gaaacgactc
    22561 aaaccagcac gattgcgtcg aaagactctc gtagtgcctg gcgcaagaca gacaccatgt
    22621 ggatgctggg cctttacggc acggcaatcg gcgcgggcgt gctgttcctg ccaatcaacg
    22681 ccggtgttgg cggtatgatc ccgctgatca tcatggctat ccttgcgttc ccgatgacgt
    22741 tttttgctca ccgcggcctg actcgcttcg tactgtctgg taaaaacccg ggcgaagaca
    22801 tcaccgaggt tgtagaagaa cactttggta ttggcgcagg taaactgatt accctgctct
    22861 acttcttcgc tatctacccg atcctgctgg tttatagcgt ggcaatcacc aatacagttg
    22921 aaagcttcat gtctcaccag ctgggtatga cgccaccgcc gcgtgcgatt ctgtcgctga
    22981 tcctgatcgt gggtatgatg accatcgttc gctttggtga gcagatgatc gttaaagcga
    23041 tgagtattct ggtattcccg tttgttggcg tactgatgct gctggctctg tacctgatcc
    23101 cgcagtggaa cggcgcagca ctggaaacgc tgtctctgga cactgcatct gcaaccggaa
    23161 acggtctgtg gatgaccctg tggctggcaa ttccggtaat ggtgttctcg ttcaaccact
    23221 ctccgatcat ctcttctttc gccgttgcga agcgtgaaga gtacggcgat atggcagaac
    23281 agaaatgctc gaagatcctg gcattcgcac acatcatgat ggtgctgacc gtaatgttct
    23341 tcgtcttcag ttgcgtactg agcctgactc cggcagacct ggctgcggct aaagagcaga
    23401 acatctcgat tctgtcttac ctggctaacc actttaacgc accggttatc gcgtggatgg
    23461 ctccgattat cgcgattatc gctatcacca aatccttcct cggccactac ttgggcgcac
    23521 gtgaaggctt caacggtatg gtgattaaat ctctgcgtgg taaaggtaag tctatcgaaa
    23581 tcaacaagct gaaccgtatc actgcgctgt tcatgctggt aacgacctgg attgttgcca
    23641 ccctgaaccc gagcatcctg ggtatgattg aaaccctggg cggcccaatc atcgcgatga
    23701 tcctgttcct gatgccgatg tacgcaattc agaaagtacc ggcaatgcgt aagtacagcg
    23761 gtcacatcag caacgtattc gttgtcgtga tgggtctgat tgcaatctcc gcaatcttct
    23821 actctctgtt cagctaagtc ctttcgcgcc gctttcgggc ggcgcttcct ccgttttaac
    23881 gcgatgtatt tcctatgatt agcgtattcg atattttcaa aatcggcatt ggcccttcca
    23941 gttctcatac cgttggacca atgaaagcgg gtaaacaatt taccgacgat ctgattgccc
    24001 gtaatctgct taaagacgtg acccgcgtgg tggttgacgt gtacggctcg ctctctctga
    24061 ccggtaaagg ccaccacact gatatcgcca ttattatggg cctggcgggt aacctgccgg
    24121 ataccgtgga tatcgattcc atccctggtt ttattcagga tgtgaatact catggtcgcc
    24181 tgatgctggc aaacggtcag catgaagtgg agttcccggt tgatcagtgc atgaacttcc
    24241 atgcagacaa cctttctctg catgaaaacg gtatgcgcat taccgcgctg gcgggcgata
    24301 aagtcgttta cagccagact tactactcta ttggcggtgg ctttatcgtt gatgaagagc
    24361 attttggcca gcagaatagc gcaccggttg aagttcctta tccgtacagt tcagcagccg
    24421 atctgcaaaa acattgtcag gaaaccgggc tgtcactctc tggcctgatg atgaaaaacg
    24481 agctggcgct gcacagcaaa gaagagctgg aacagcacct ggcgaacgtc tgggaagtca
    24541 tgcgcggcgg tattgagcgc ggtatttcta ccgaaggcgt gttgcctggc aaactgcgcg
    24601 taccacgccg tgctgcggca ctacgccgga tgctggtcag ccaggataaa accaccactg
    24661 acccgatggc ggttgttgac tggatcaaca tgtttgcact ggcagtgaac gaagagaacg
    24721 ccgctggcgg tcgcgtggtg actgcgccga ctaacggtgc gtgcgggatt atcccggctg
    24781 tgctggcgta ctacgataag tttatccgcg aagtgaacgc taactcactg gctcgttacc
    24841 tgctggtagc cagtgccatt ggttctcttt ataagatgaa cgcgtcaatt tctggtgctg
    24901 aagtcggctg ccagggtgaa gttggtgtgg cgtgctcaat ggcggcggct ggtctggcag
    24961 aactgttagg tgcaagcccg gcgcaggtgt gcatcgcggc ggaaatcgcc atggagcaca
    25021 acctcggtct gacgtgtgac ccggtcgccg gacaggtcca ggtgccatgc atcgagcgta
    25081 acgccattgc ggcagtaaaa gcggtgaacg ccgcacgtat ggcgctacgc cgtaccagcg
    25141 agccgcgcgt ctgcctcgat aaagttatcg aaaccatgta cgaaacaggt aaagatatga
    25201 acgccaagta ccgcgaaacc tctcgcggcg gcctggcaat gaagatcgtt gcctgcgatt
    25261 aatcgctctc cagaggcctc gttttgcgag gcctcttccc gatttctcat ccagccgtag
    25321 cctgttccgg catcgaatgt tacccttatc gcctgatctt taagggggtt atcgtggctg
    25381 ttcatttgct tattgtcgat gcactgaatc ttattcgtcg cattcatgcc gttcagggat
    25441 cgccctgtgt cgaaacctgc cagcatgcgc tcgatcagct cattatgcac agccagccaa
    25501 cccacgcggt cgccgttttt gatgatgaaa accgcagtag cggctggcgt catcagcgtt
    25561 taccagatta caaagcgggt cgaccgccaa tgccggaaga gttgcacgac gagatgcctg
    25621 cattacgcgc cgcctttgag caacgcggcg tcccgtgctg gtcaaccagc ggcaacgaag
    25681 ccgatgactt agccgccacg ctggcggtca aagtgacaca ggccgggcat caggcaacga
    25741 ttgtttcgac agataaaggc tactgtcagt tactttcacc gacattacgt attcgtgatt
    25801 acttccagaa acgttggctg gatgcgccat ttatcgataa agaatttggc gttcaaccgc
    25861 agcagttgcc cgattactgg ggacttgcgg ggatcagcag ttcaaaggta ccgggtgttg
    25921 cgggaatcgg accaaaaagc gccacgcagc tgctggtcga gtttcagagt ctggaaggga
    25981 tatatgagaa tctggatgcg gttgccgaaa agtggcgcaa aaaattagaa acccataaag
    26041 agatggcgtt tctgtgccgc gatattgccc gcttacaaac cgatttgcat atcgacggca
    26101 atttacagca attgcggttg gtacggtaac ggcgagccgg atacgccgca aacgtcgtat
    26161 ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac aatatcctca
    26221 agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc ctgcgccagt
    26281 gccggaatgt cttccttgcg tacaccaaca tcacgcaaat gtggcggaat accgacatca
    26341 cggttgagag caaacaccgc ttcaacagcg gcattacgcg cctcttccag gctcatacct
    26401 tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt aaagtcagcg
    26461 ttatagcgca tgacatgcgg taacaggatg gcgttcgcaa caccgtgtgg agtgttgtaa
    26521 aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt cgagaagccc
    26581 atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc accagcaacc
    26641 gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc atcggttagc
    26701 gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc gacacccgtc
    26761 gcagctttca gcgctggagg cataccatcc atcatgtcag cgtcaataaa cgccacctgc
    26821 gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tctcttcgtc agtgatcacg
    26881 tagttaatgg tcacttctgc cgcagtacct gctgtggtag gaattgccag aatcggtaca
    26941 ctgggtttat tggtcgggga aagcccttcc aggctacgca catcggcaaa ctccgggttg
    27001 ttgctgataa tgccaatcgc tttacaagta tcctgtggag aaccaccacc aatagcgatc
    27061 aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac agtaattgtt
    27121 gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc catcttatcg
    27181 gtcactttcg ccaccacgcc gcattgcacc agcgttttat cggtgacgat cagcgccttc
    27241 tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg accaaaccat
    27301 gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc tttacgaaat
    27361 tactcttcaa tccgtaaccc ataggttttg aatttctcca gcactacggc aatctcttca
    27421 tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag ttgcgccagc
    27481 acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca agcgataagc
    27541 ccatgatgtt gtaacaaagt tgccttacga tttttgagag cctgcgcaac atgttcagaa
    27601 agttcacgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc gccagccgcc
    27661 gcaatcatgt agtgaatagc ggggatcgat cggttaagaa tggaaactgc cgtgcaatga
    27721 acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc cgccatatgg
    27781 aaacgccatt cgcttgaggg gagctttcct tcctcatgtt taccgttgcc atcaataaag
    27841 acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat cagcatccca
    27901 tcctgataac gtacactgac gttccccgct gtcccctggt tcagtcccag gcgggtcatt
    27961 tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag ctacctctct
    28021 ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag ccgtttaatt
    28081 acccaccatc ttcttcctga ttaaccagaa agaaattcac aagcttatat tttgtgacct
    28141 ggttcaacta atcacagtaa ataactgcaa gttctctttt tataacccca ttaaaaatga
    28201 ccgctcttaa aaatatttat caaaacggtc atttttctat tcactcaagc caggaatgcc
    28261 cgttttcggc ataaacaatt aatacggtca tctggtttgt gttttttatg atttattttc
    28321 tgaaacgggc atgaaatttc gattattaaa gtgatggtag tcacataaag tcaccttcca
    28381 gctaataagt gtgaccgccg tcatattaca gagcgttttt tatttgaaaa tgaatccatg
    28441 agttcatttc agacaggcaa atattcactg atatgaagcc cgaactcgct ggttttgcac
    28501 ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac ctaagaggat gctatgggaa
    28561 acacatcaat acaaacgcag agttaccgtg cggtagataa agatgcaggg caaagcagaa
    28621 gttacattat tccattcgcg ctgctgtgct cactgttttt tctttgggcg gtagccaata
    28681 accttaacga cattttatta cctcaattcc agcaggcttt tacgctgaca aatttccagg
    28741 ctggcctgat ccaatcggcc ttttactttg gttatttcat tatcccaatc cctgctggga
    28801 tattgatgaa aaaactcagt tataaagcag ggattattac cggattattt ttatatgcct
    28861 tgggcgctgc attattctgg cccgctgcag aaataatgaa ctacacctta tttttagttg
    28921 gcctatttat tattgcagcc ggattaggtt gtctggaaac tgccgcaaac ccttttgtta
    28981 cggtattagg gccggaaagc agtggtcact tccgcttaaa tcttgcgcaa acatttaact
    29041 cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct tattttgtct aacgtgccac
    29101 atcaatcgca agacgttctc gataaaatgt ctccagagca attgagtgcg tataaacaca
    29161 gcctggtatt atcggtacag acaccttata tgatcatcgt ggctatcgtg ttactggtcg
    29221 ccctgctgat catgctgacg aaattcccgg cattgcagag tgataatcac agtgacgcca
    29281 aacaaggatc gttctccgca tcgctttctc gtctggcgcg tattcgtcac tggcgctggg
    29341 cggtattagc gcaattctgc tacgttggcg cacaaacggc ctgctggagc tatttgattc
    29401 gctacgctgt agaagaaatt ccaggtatga ctgcaggctt tgccgctaac tatttaaccg
    29461 gaaccatggt gtgcttcttt attggtcgtt tcaccggtac ctggctcatc agtcgcttcg
    29521 caccacacaa agtcctggcc gcctacgcat taatcgctat ggcactgtgc ctgatctcag
    29581 ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt atgcagcgcc tttatgtcga
    29641 ttcagtaccc aacaatcttc tcgctgggca ttaagaatct cggccaggac accaaatacg
    29701 gttcgtcctt catcgttatg accatcattg gcggcggtat tgtcactccg gtcatgggtt
    29761 ttgtcagtga cgcggcgggc aacatcccca ctgctgaact gatccccgca ctctgcttcg
    29821 cggtcatctt tatctttgcc cgtttccgct ctcaaacggc aactaactga acatattttc
    29881 cgaataaagt gaggaatctg taatgaaaaa aatcagctta ccgaaaattg gtatccgccc
    29941 ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt gaagaacaaa caatgaatat
    30001 ggcgaaagct acggccgcac tgctgaccga gaaactgcgc catgcctgcg gagctgccgt
    30061 cgagtgtgtc atttccgata cctgtatcgc gggtatggct gaagccgctg cttgcgaaga
    30121 aaaattcagc agtcagaatg taggcctcac cattacggta acgccttgct ggtgctatgg
    30181 cagtgaaacc atcgacatgg atccaacccg cccgaaagcc atttggggct ttaacggcac
    30241 tgaacgtccc ggcgctgttt acctggcagc ggctctggca gctcacagcc agaaaggcat
    30301 cccggcattc tccatttacg gtcatgacgt tcaggatgcc gatgacacat cgattcctgc
    30361 cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt ttggccgtcg ccagcatgaa
    30421 aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt atcgccggtt ctattgttga
    30481 tcacaacttc tttgaatcct ggctgggaat gaaagtccag gcggtggata tgaccgaact
    30541 gcgtcgccgt atcgatcaga agatttacga cgaagccgaa ctggaaatgg cactgacctg
    30601 ggctgataaa aacttccgct atggcgaaga tgaaaataac aaacagtatc aacgcaatgc
    30661 cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg gcgatgtgta tccgcgacat
    30721 gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg gaagaatcac ttggctacaa
    30781 cgccatcgct gcgggcttcc aggggcaacg tcactggacc gatcaatatc ccaatggtga
    30841 caccgccgaa gcgatcctta acagttcatt tgactggaat ggcgtgcgcg aaccctttgt
    30901 cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg ctaatgggtc accagctcac
    30961 cggcaccgct caggtatttg ccgatgtgcg tacctactgg tcaccagaag caattgagcg
    31021 tgtaacgggg cataaactcg atggactggc agaacacggc atcatccatt tgatcaactc
    31081 cggttctgct gcgctggacg gttcctgtaa acaacgcgac agcgaaggta acccgacgat
    31141 gaagccgcac tgggaaatct ctcagcaaga ggctgacgct tgcctcgccg ctaccgaatg
    31201 gtgcccggcg atccacgaat acttccgtgg cggcggttac tcttcccgct tccttaccga
    31261 aggcggcgtc ccgttcacca tgacccgtgt caacatcatc aaaggcctgg gaccggtact
    31321 gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat gtgcatgaca tcctcaacaa
    31381 acgcaccaac tcaacctggc caaccacctg gtttgcaccg cgcctcaccg gtaaagggcc
    31441 gtttacggat gtgtactcgg taatggcgaa ctggggcgct aaccatgggg ttctgaccat
    31501 cggccacgtt ggcgcagact ttatcactct cgcctccatg ctgcgtatcc cggtatgtat
    31561 gcacaacgtt gaagagacca aagtgtatcg tccttctgcc tgggctgcgc acggcatgga
    31621 tattgaaggc caggattacc gcgcttgcca gaactacggc ccgttgtaca agcgttaata
    31681 cattactccc tgatgtgatg cccggtcgct ccggctaccg ggcctgaaca agcaagagtg
    31741 gttagccgga taagcaatgt tatccggcta tattgcagga gcgattatga aacaagaagt
    31801 tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc atcgcggtta atcggcaggg
    31861 aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat atcgcgatgg aaaacaacac
    31921 ctggcaccag tggtctttag acgccatttt gcaacgcttt gctgattgct gtcggcaaat
    31981 caatagtgaa ctgactgaat gccacatccg cggtatcgcc gtcaccacct ttggtgtgga
    32041 tggcgctctg gtagataagc aaggcaatct gctctatccg atcattagct ggaaatgtcc
    32101 gcgaacagca gcggttatgg acaatattga acagttgatc tccgcacagc ggttgcaggc
    32161 tatttctggc gtcggagcct ttagtttcaa tacgttatat aagttggtgt ggttgaaaga
    32221 aaatcatcca caactgctgg aacgcgcgca cgcctggctc tttatttcgt cgctgattaa
    32281 ccaccgttta accggcgaat tcactactga tatcacgatg gccggaacca gccagatgct
    32341 ggatatccag caacgcgatt tcagtccgca aattttacaa gccaccggta ttccacgccg
    32401 actcttccct cgtctggtgg aagcgggtga acagattggt acgctacaga acagcgccgc
    32461 agcaatgctc ggcttacccg ttggcatacc ggtgatttcc gcaggtcacg atacccagtt
    32521 cgcccttttt ggcgctggtg ccgaccaaaa tgaacccgtg ctctcttccg gtacatggga
    32581 aattttaatg gtccgtagcg cccaggttga tacttcgctg ttaagtcagt acgccggttc
    32641 cacctgcgaa ctggatagcc aggcagggtt gtataaccca ggtatgcaat ggctggcatc
    32701 cggcgtgctg gaatgggtga gaaaactgtt ctggacggct gaaacaccct ggcaaatgtt
    32761 gattgaagaa gctcgtctga tcgcgcctgg cgcggatggc gtaaaaatgc agtgtgattt
    32821 attgtcgtgt cagaacgctg gctggcaagg agtgacgctt aataccacgc gggggcattt
    32881 ctatcgcgcg gcgctggaag ggttaactgc gcaattacag cgcaatctac agatgctgga
    32941 aaaaatcggg cactttaagg cctctgaatt attgttagtc ggtggaggaa gtcgcaacac
    33001 attgtggaat cagattaaag ccaatatgct tgatattccg gtaaaagttc tcgacgatgc
    33061 cgaaacgacc gtcgcaggag ctgcgctgtt cggttggtat ggcgtagggg aattcaacag
    33121 cccggaagaa gcccgtgcac agattcatta tcagtaccgt tatttctacc cgcaaactga
    33181 acctgaattt atagaggaag tgtgaaatgc tgaaaacaat ttcgccgtta atttctcccg
    33241 aactattgaa agtgctggca gagatgggac atggagatga aattattttt tccgatgctc
    33301 actttcccgc ccattcgatg ggaccgcagg tgatccgcgc tgatggcctg ttggtgagcg
    33361 acttgctcca ggcgattatc ccgttatttg agctggacag ttatgcaccg ccgctggtga
    33421 tgatggcagc ggtagaaggt gacactctcg atcctgaagt agaacgacgt taccgtaatg
    33481 cgctttcact acaagccccg tgtcctgaca tcatccgcat caatcgtttt gcgttttatg
    33541 aacgggcgca aaaagccttt gcgatcgtta tcacaggcga acgagcgaag tacgggaata
    33601 ttcttttaaa aaaaggggta acaccgtaat ctcataccgg tacgcccgca tgacgcgggc
    33661 ggttatcgaa tgatggggtg aaaaatatga aagcggcacg ccagcaagcg atagtcgacc
    33721 tgctgctgaa ccataccagc ctgaccacgg aagctctctc tgaacagcta aaggtcagta
    33781 aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca gggtaaaatt ctgcgcaatc
    33841 atggacgcgc taaatatatc caccgtcaaa atcaagacag tggcgatccc tttcacatcc
    33901 ggctgaaaag ccattatgcg cataaagcag atatcgcgcg cgaggcgctc gcgtggattg
    33961 aagaagggat ggtgatagcc ttagacgcca gttcaacttg ctggtatctg gcacgccagt
    34021 tgcctgacat caacattcag gtcttcacca atagccatcc gatttgccat gaactcggta
    34081 aacgcgaacg cattcaactg atcagttccg gcggcacact tgagcgcaaa tatggctgtt
    34141 acgtcaatcc ctcgctgatt tcccaactta aatcgctgga aatcgatctg tttatttttt
    34201 cttgtgaagg gatcgatagc agcggcgcat tgtgggactc caatgccatc aacgctgatt
    34261 acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt attgattgat aaaagtaaat
    34321 ttaatcgttc aggggaagcc cgcatcgggc atctggatga ggtaacgcac attatttctg
    34381 atgagcgcca ggttgcaact tctttggtaa cagcctgacg gaaagggtag caggccggag
    34441 acgacgcccc ggccttgcct gttatcgctc gtcgcgacga ccacccaccg cagcccagat
    34501 gcggcggacg tgcaccgtca cttcttcgcg atcgtgatac aactgccgcg cctgaatctg
    34561 agcatttatg ccatgttcat caagctgtgc ctgaatatac gccagattgt gtgacacttc
    34621 ttcgtagcgt tttttcatcg gcagtttgag gttgaaaatg gtttcacggc accagccatt
    34681 aaccagccac tgcgccatca atgccgcaac tttcgccggt ttttcaacca tatcgcatac
    34741 catccaggag atattgctgc gcgtcggacg gaatttgaaa ccgtcttccc gcagccacgt
    34801 cacctgtccg gtatccatca gactttgcgc catcgggccg ttgtcgacgg aataaaccca
    34861 catgttgcgc ttcaccagtt ggtaggtcca gccgccaggg caagcgccta aatccaccgc
    34921 ccacatcccg ttcgccaggc gttcatccca ctcatccgca ggaataaaca catgaaatgc
    34981 ctcttccagt ttgagcgtgg aacgactcgg cgcatctgcc ggaaatttca ggcgcggaat
    35041 gcccatatag aacggcgaat tattgttgct gtatgagtaa ccggtatagc agcagcctgg
    35101 tgcaataaag aatacatgca caaccggacg cttcggcgtt tcatagttcg ccagcacccc
    35161 ggcatcgcgc aaggcagcgc gtagcggaac agtaaattta cggcagaatt tcagtaactc
    35221 tttgctttcg ttggtatcgg caacttcaac acgcagttca ccgcccttct ctactacgcc
    35281 ctgtaacatg ccgacaatgg gggtaatacg atcttctggc ggcaaatgct gcaggagttc
    35341 ccccaccaca aaccactggc gggcaaaaat taatgaactg aacggcagct cacggattaa
    35401 cttatcgcca tcatcaggtt gataacattc ataaatgaca taacccgcat tctctttcac
    35461 gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg gtaatttctg cggcgcactc
    35521 tttttcaaag cccggacggc acagcaatac aaccttattc atgacttacg cccttgcgct
    35581 ttaaacggat agcaccaact aacattaacg cccagcccgc gaggaagctc acgccgccaa
    35641 ccggagtgac aaacgcccac aaacgcagat gggacagcgc caggcaataa aggctgccgc
    35701 tgaacaacac cgtgcctaac gcgaggaaaa cgctactcca gtaaaaccag atactgatgc
    35761 gacgctgcat tgccaccgcc agacctaaga tcgccagcgt atgaaacgcc tggtattcga
    35821 ggccggtctg gatccagccc atctcaacgg cccccatggt tttacttaac acatgcgcgc
    35881 caaaagcgcc cagagccaca aaaatgaagc cgctaatggc ggcgaaaatc agcataaaac
    35941 ggctggtcat ggtcgtaccc tacgtaaatt attgttcata acgaaagcgg aatttttctt
    36001 gttcagcagc agctttcgcc aggatccatt gacgaaaggc ggctatttta cccagttctg
    36061 cctggctgtc atgacaaacc agataaaaag cgtttttact gaccagaaca tcattaaacg
    36121 ggcaaacaag acgtccggcc tcgatttcag attgcgccat cacgttattt gccagcgcca
    36181 ctccctgccc gtgaatagcc gcttgcagca ccatggcgct atggctaaaa attggccctt
    36241 gctgaacgtt gatatgattt aaccccaatt gtcgggtata tgtctgccag tcacggcgtg
    36301 acgcatcatg taataacgta tgtttagcca gatcttccgg ggtcttcaag ggtttttcgc
    36361 cagtcagcag tagcggcgaa cacaccggca ataaatattc ggcgtaaagt ttttccaccc
    36421 gtagccccgg ccagttgccc cgaccataaa atatcgccac atcaacatca tccgccagct
    36481 tatcttcctg acgatcaacc gcctggattc gaacgtcaat tcccggataa gctgaattaa
    36541 agctggaaag tcgcggaacc aaccaatgaa tggcgaaact ggggagtaaa ctgaccgtca
    36601 acgccccctt ggcgctacgg gcctggagtt tacgcgtcgc ttcggttaat tgcgaaaata
    36661 tctctttgat atcgaggaaa tagctttgcc cttcctcggt cagcaggagt gaacgattac
    36721 ggcggcggaa cagttttagc cccaaaaaat cctcaagaga cttgatttga tgacttactg
    36781 cggcctgggt cacaaaaagc tcttctgctg cgcgagtgaa acttaaatgg cgtgctgcgg
    36841 catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt agacatggct tttaaacttt
    36901 gatgttaaat taatttaaca attagatcac actatgtaac ctattagttt ttttaatctg
    36961 agccattata aattgtccgt tgagcttcta ccagcaaata cctatagtgg cggcacttcc
    37021 tgagccggaa cgaaaagttt tatcggaatg cgtgttctgg tgaacttttg gcttacggtt
    37081 gtgatgttgt gttgttgtgt ttgcaattgg tctgcgattc agaccatggt agcaaagcta
    37141 ccttttttca cttcctgtac atttaccctg tctgtccata gtgattaatg tagcaccgcc
    37201 taattgcggt gctttttttt accttgcgat cgcgaattac tgatccagtt cgaccatctc
    37261 tttcacgtca gtacggttga tctgctgttt gttgccatta gcgtctttat acgaaatcat
    37321 accggtatcg ttatcagtct gtggtttgcc gtcagagacg atggtacgtc cgtcattggt
    37381 gtgcatcaca tagttcgaac cggaacaggc gctcagggca aaagtcagca tacaggcaga
    37441 aataattgcg gcagtctttt tcatcatctt ctccttaaag cgattaataa atcttaatag
    37501 tattgattaa caggctaaaa ttaacgccta acactattca gcatatgtta cttacgcggc
    37561 ttcgccagga tatccagata attctgatgg ttagcactct ccttgtatca aagtgaattt
    37621 tgcgtcacga tcggtgcatc aagccgagga gtaccatgaa cgtttttaat cccgcgcagt
    37681 ttcgcgccca gtttcccgca ctactggatg cgggcgtcta tctcgatagc gccgcgaccg
    37741 cgcttaaacc tgaagccgtg gttgaagcca ctcgacagtt ttacagcctg agcgccggaa
    37801 acgtccatcg cagccagttt gccgaagccc aacgcctgac cgcgcgttat gaagctgcac
    37861 gagagaaagt ggcgcaatta ctgaatgcac cggatgataa aactatcgtc tggacgcgcg
    37921 gcaccactga atccatcaac atggtggcac aatgctatgc gcgtccgcgt ctgcaaccgg
    37981 gcgatgagat tattgtcagc gtggcagaac accacgccaa cctcgtcccc tggctgatgg
    38041 tcgcccaaca aactggagcc aaagtggtga aattgccgct taatgcgcag cgactgccgg
    38101 atgtcgattt gttgccagaa ctgattactc cccgtagtcg gattctggcg ttgggtcaga
    38161 tgtcgaacgt tactggcggt tgcccggatc tggcgcgagc gattaccttt gctcattcag
    38221 ccgggatggt ggtgatggtt gatggtgctc agggggcagt gcatttcccc gcggatgttc
    38281 agcaactgga tattgatttc tatgcttttt caggtcacaa actgtatggc ccgacaggta
    38341 tcggcgtgct gtatggtaaa tcagaactgc tggaagcgat gtcgccctgg ctgggtggcg
    38401 gcaaaatgat tcacgaagtg agttttgacg gtttcaccac tcaatctgcg ccgtggaaac
    38461 tggaagcagg aacgccaaat gtcgctggcg tcataggatt aagcgcggcg ctggaatggc
    38521 tggcagatta cgatatcaac caggccgaaa gctggagccg tagcttagca acgcttgcag
    38581 aagaagcgct ggcgaaacgt ccaggctttc gttcattccg ctgccaggat tccagcctgc
    38641 tggcctttga ttttgctggc gttcatcata gcgatatggt gacgctgctg gcggagtacg
    38701 gtattgcctt gcgggccggg caacattgcg ctcagccgct actggcagaa ttaggcgtaa
    38761 ccggcacact gcgcgcctca tttgcgccat ataatacaaa gagtgatgtg gatgcgctgg
    38821 tgaatgccgt tgaccgcgcg ctggaattat tggtggatta atgacaaacc cgcaattcgc
    38881 cggacatccg ttcggcacaa ccgtaaccgc agaaacgtta cgcaatacct tcgctccgtt
    38941 gtcgcaatgg gaagataaat atcgccagtt gatcatgctg gggaaacagc ttccggcttt
    39001 gccagacgag ttaaaagcgc aggctaaaga gattgccgga tgcgaaaacc gcgtctggct
    39061 gggatatacc gtgactgaaa acggtaaaat gcacttcttt ggcgatagcg aagggcgcat
    39121 tgtgcgcggc ctgttggcgg tgttgttgac tgccgttgag gggaaaaccg ccgccgaatt
    39181 gcaggcacag tcgccgctgg cattgtttga tgagctggga ttgcgtgcac agcttagcgc
    39241 ctcacgcagc caggggttga atgcgttaag cgaggcgatt atcgctgcgg cgaagcaggt
    39301 ttaagcacag aaccaggtcg gataagacgt tcgcgtcgca tccgacctga ttgtttaacc
    39361 ctgacgcgcc gctttcgcca tcatcttctt cagcgcatga gaaaccgcaa caaacccaaa
    39421 ggtggcggtc accatcgttg ccgcgccaaa tccagatgca caatccatcc gcttcggccc
    39481 ttctgccgtg gctttcatcg cacacacagt accgtctgac tgcgggtaca ccagcgcttc
    39541 agtagaaaac acgcaatcga cgccgagctt acctttactg tttttcacta cgccaaaatc
    39601 gcttttcagg cgctcgcgca acttcgccgc cagcggatcc tgaatcgttt tcgccagatc
    39661 ggtaacctga atctgcgtcg ggtcaatctg cccacccgcg ccaccggtcg tgaccagcgg
    39721 gattttattg cgccgacaat aagcaatcag cgccgctttg ggccgtacac tatcaatggc
    39781 atcaatcacg tacgaataac ccacgctcat atactgcgct acgttatccg gcgtcacgaa
    39841 atcatccacc accgttacac ggcactccgg gttaatctgg cgaatacgct ccgccatgac
    39901 ttccgctttt gccagcccga cgttatcgcg cagagcgtga atttgccgat tggtattggt
    39961 gacgcacaca tcatccatat cgataagcgt gattgcgcca atccccgtgc gcgccagcgc
    40021 ttccgccgcc caggaaccga caccaccgat accaaccaca caaatatgcg cgtcagcaaa
    40081 cagttgcaac gctttttcac catacagacg cgctgtgcca ccaaaacgct gacgccatgc
    40141 atcactaatt accacagaca tagcacctca gaattaaaaa gggtgaggtt atccctcacc
    40201 ctggcgtatc cgtaaaaacg gtatacagaa taacacatca gccgctaaag acgttacctg
    40261 cgcccggggc ggttttcagc acccagacac gtccatagtg gttgtaccaa cctgcgcgat
    40321 gtccggcttc cggcccgatc ccttgataga tatcgaagtg ttggcctttg attgcgccac
    40381 cgacatccag cgccaccatc agacgcaatt cgtactgacc gttaaatttg ccgttattat
    40441 ccagcagcgg cacttctgcc agcaaggtag tacctggcgg aataattgaa cgatcggagg
    40501 caacagaagc acggccaacc agcggcaccg cacttgcccc tttcaccggt gcaaaagatt
    40561 gcggtttaaa gaaaacgaaa gacgggttct gttccagcag ctcgcgaacc tcggcttcac
    40621 tgtgtgtttc gccccaatga cgaatcgcct gcatcgacat atcttctttt ttcacttcgc
    40681 cacggtcgat cagcacctta ccaatgctgc gataggcatg accgtttttc cctgcatagc
    40741 tgaaaaagtt aagcggactg ccatcaccaa agtcgatata cccgctaccc tgcacatcca
    40801 taatgaagtt atccatcagg gagttgctgt aagcgagaat atatttatca ctcagcgccc
    40861 ctgcgtagat ctccgcacga gacggcagac gaccacgttt tggcggcata cggtaaatcg
    40921 gatactggaa ctcgccctgg cgggtatggc gcgcctgaat taccggcgtg taatagcctg
    40981 taaactgcac gttaccatag ttgtcggcac cttccatctg ccaggcatca atgccgaact
    41041 ggcgcatatt gcgggtatca ccgcctgcgc gcagccactc ttgcaccgcg ttataaacat
    41101 tactctggtt gccatacaga cgcggtgacg aattacggat atggttaatt tgctcggcaa
    41161 aatcaccggc gttaatcggc gcgccaacgg catctggctg gttcaccaga gagaaaggct
    41221 gggtaaattt cccgtcttta tattgctgtc cgcgatcggt tggtttggaa gagcaggcgg
    41281 caagcattgc cacaaccgtg cccataagaa ggtactttac ccaacgtcct ttcattgttc
    41341 tcttcttcag gttaaaaaat aaggcgcaac gaagataaca aaccgccgcg tttaatgaaa
    41401 tgagcagcct ctccctgacg caaattttgc acaaaaaata ggctttagtg atttgttttt
    41461 gttcaaaatc atgccaaatc cgtgatcggg gtaaaaaaaa ggttgcatga aaacgcgagc
    41521 ggagtatagt gcgcatccac ggacgcgggg tggagcagcc tggtagctcg tcgggctcat
    41581 aacccgaagg tcgtcggttc aaatccggcc cccgcaacca attattgaac accctaacgg
    41641 gtgttttttt gtttctgctc tcccataaaa aaagcgccat tcagcgcctt tttatcatcc
    41701 ccttctcgcc agcgtcgccc catcggcaaa atacgcttta attcccgcaa gaatagactc
    41761 cgcaacttcc tgctggaaag tcgccgtttt cagtttacgc tcttcctcaa cgttactgat
    41821 aaacgccgtt tcgaccagaa tggagggaat atcaggcgct ttcagcacgg caaacccggc
    41881 ctgttcaacc tgatttttat gcagcttgtt gattttaccg agcttattca gcaccgcttt
    41941 accaaactta aggctgtcgg caatggtcag cgactgcacc atatcgaaca tggtgtggtc
    42001 gacatagcgg tcaccgcttt tgctcacgcc accaatcaag tccgaggcgt tctgggtctg
    42061 tgccagatat tttgccgcag tactggttgc gcctttggtt gagagcgcaa acactgagga
    42121 accgctcggc tggcgactgg taaaggcgtc ggcatggata gagacaaaca ggtcggcacg
    42181 ctgtttctgg gcttttgcta cgcgcacttg caacggaatg aagatgtctt cattgcgcgt
    42241 catgtacacc ttcatattgc cctctttctc gatcagagag cgcagacggc gagctatttg
    42301 caataccacg tctttttcgc gcgttttgta tttccccacc gcaccggagt cttcgccgcc
    42361 gtggccaggg tcaagcataa taacaatagg acgatcgcgc cctgctttac ccggttgtgg
    42421 accactttgt gctggcggca cctgcttttc gaggtcgcct ttgttgtaat cctccagcag
    42481 cgccagcagc gggtcctgca tatcctgtgc attggccgga tagaggtcca tcaccagacg
    42541 ctctttaaac ccggcgaccg gcgcaagggc aaacagctgc ggttttacgt tttgctttaa
    42601 ttcaaaaacc atccgtacgg tctgcgggtc aaattgcccg acgcgcgccg acttgatgaa
    42661 cgggtcgtct gcacgaattt gtgccgccat ccccttgagt accgagttca ggtttacatc
    42721 ttcgatatcc accaccacgc gttccgggtt actcaacgcg aactgcttat atttcagctg
    42781 acgatttgat tctaccgtca cgcgggtgta gctggacgca ggccagacgc gcaccgccac
    42841 gacctggcta accgcagcca ggctgacctg acttacgctc aatagccaca tagcgcccgc
    42901 gccttgcagt aaacgacgac ggctgattgc agtgttggat cctgacatgc ctctcccgag
    42961 caaaagaaat ctaagctgtg taacaagtaa acgactaatt tgaccggaaa ctttagcgaa
    43021 agacgcataa tctgtcatct aataaacggt aaacattctt tttatattca cggcattact
    43081 gataaaaaag tcgctctcgc ataaaattta cacttgcacc ctgcgaaaaa acagaataaa
    43141 aatacaataa tttcgaataa taatgcaaag gggtgtgccg tggtaaagga acgtaaaacc
    43201 gagttggtcg agggattccg ccattcggtt ccctatatca atacccaccg gggaaaaacg
    43261 tttgtcatca tgctcggcgg tgaagccatt gagcatgaga atttctccag tatcgttaat
    43321 gatatcgggt tgttgcacag cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg
    43381 cagatcgacg caaatctggc tgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt
    43441 gtgaccgacg ccaaaacact ggaactggtg aagcaggctg cgggaacatt gcaactggat
    43501 attactgctc gcctgtcgat gagtctcaat aacacgccgc tacagggcgc gcatatcaac
    43561 gtcgttagtg gcaattttat tattgcccag ccgctgggcg tcgatgacgg cgtggattac
    43621 tgccatagcg ggcgtatccg gcggattgat gaagacgcga tccatcgtca actggacagc
    43681 ggtgcaatag tgctaatggg gccggtcgct gtttcagtca ctggcgagag ctttaatctg
    43741 acctcggaag agattgccac tcaactagcc atcaaactga aagctgaaaa gatgattggt
    43801 ttttgctctt cccagggcgt tactaatgac gacggtgata ttgtctcaga acttttccct
    43861 aacgaagcgc aagcgcgggt agaagcccag gaagagaaag gcgattacaa ctccggtacg
    43921 gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta
    43981 atcagttatc aggaagatgg cgcgctgttg caagagttgt tctcacgcga cggtatcggt
    44041 acgcagattg tgatggaaag cgccgagcag attcgtcgcg caacaatcaa cgatattggc
    44101 ggtattctgg agttgattcg cccgctggag cagcaaggta ttctggtgcg ccgttctcgc
    44161 gagcagctgg agatggaaat cgacaaattc accattattc agcgcgataa cacgactatt
    44221 gcctgcgccg cgctctatcc gttcccggaa gagaagattg gggaaatggc ctgtgtggca
    44281 gttcacccgg attaccgcag ttcatcacgg ggcgaggttc tgctggaacg cattaccgct
    44341 caggcgaagc agagcggctt aagcaaattg tttgtgctga ccacgcgcag tattcactgg
    44401 ttccaggaac gtggatttac cccggtggat attgatttac tgcccgagag caaaaagcag
    44461 ttgtacaact accagcgtaa atccaaagtg ttgatggcgg atttagggta agcgatgaaa
    44521 attgtcggat gcgacatgcg taacactcgt gcgtcgcatc cggcaattac gtttattccc
    44581 gcgaactaaa caacgccgcc agaccactgc gccgctcagt acgagtggcg attgccgcac
    44641 ttaatatacg ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc accgcggtgt
    44701 ataccagctc tcgcgttact accggcgtgc gttggctcgg taaaatcaac gccgcatggt
    44761 cgaactccga tccctgcgat ttatgtaccg tcatcgccca cgtagtttcg tgctctggca
    44821 agcgactcgg ttgcacagac ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc
    44881 cctgcccgcg atccagcgca ataccgatat cgccattaaa caacccaagc gcgctgtcat
    44941 tacgggcaat cattaccggt cgaccttcgt accaacgaga gtgcggatga cgatgaattt
    45001 tgcgcttctg ttgcataaac tgctcaattc gctcattcag tccagccacg ccaaacggcc
    45061 cttcccgcag ggcgcacaaa agttggtact cattgaacgc ctgaatgatt aaatccggct
    45121 cggcacgcgc ttgcagcaga tccagataac gtccataacc cgcaagagct tcctcaagca
    45181 tcgcaatata atcttctccg ctctgtaaaa gccgtttttc gatatcagta aaatcctgct
    45241 gaaaaacggt tttcactgcc gttttatcac cacggttgat cgccgcagct aactgaccaa
    45301 tgccagaatc actgccgaaa cgatagcttt tttgcagcag gcagagactg tcgcgcaaag
    45361 atgccgcttc tgtgccagtt cctgccggaa cgtgagttcc cgtcaggcgg cttagctgcc
    45421 cggcacgctc ggcggtaaag ccggcattgg cataagcgca gatatcgccc agcacagccc
    45481 cagcctcaac cgaggccagt tgatcacgat cgccgagaaa gatcactcgc gcatgatcgg
    45541 gcaaggcgtc gatcagtctc gacatcatag gcagatcgat cattgacgct tcatctacca
    45601 ccagcacatc aagatgcagc gggttaccgg catgatgacg taaacgctgg ctacccggct
    45661 gcgcacccag caatcggtgc aaagtactgg catcttccgg aatgcgtttc ttttgttcat
    45721 cggtcagcgg taactgtcgc aaagccttgc cgagagattc ggttaagcgc gcggcagctt
    45781 tacccgttgg tgcagccaga cggatacggc agcgttcgcc gtcggccatt tgaattaacg
    45841 ctgccagtaa cttcgccacg gtggtcgttt taccggtgcc tgggccgccg gaaatcaccg
    45901 agatccgccg cgtcagcgcc actgccgctg caactttttg ccagttaatt tcatcgctta
    45961 ctggaaaaag tttgtccagg gtttgcgcca gtagagcttc atcaacctca atggcatgat
    46021 tcacctcatt gaagaagcgc gctaccgtcc gctcgttaca ccacatgcga ttcaaataaa
    46081 gacgatcgcc acagaggatc atcggcgtgg gttcatctcc cctgctgacc gcttgagaag
    46141 ccagcaagca ttcttcccaa ttttgtagct caccgatttc actgacacag gtcgccaaca
    46201 gcggatgcga cgcctcgtta ttttccagtc gtgaaagcgg caaacagacg tgtccctctc
    46261 cggcatcatg acttaacagt gccgccgcga gggtgacggc aggatgttca tctcccgcca
    46321 cggtcagggc aaattgcaca tccagcgggc gtagctgttt gtgctccaca gcttccagta
    46381 attgcttttg caatttcatt acgcctcctc cagggtcata ccggcaaaca tctcatccat
    46441 cagggcaatc aacccggcgt tgggtcgggt tgtgtaaatc ccttgttgcg gatgttcttt
    46501 atcaacgcca cgcaggaaca gatagataac gccgccaaag tgacgatcat agttgtaatc
    46561 agcaatgcga tggcgcagat aacgatgcag cgccagggta taaagctgat attgcagatc
    46621 atagcggtgt gcctgcatcg ctgctgccat agcctgttgg gtgtaagccg aactgtcttc
    46681 acccagccag ttggatttat agtcgagcag gtaataacgc ccttcgtggc ggaataccag
    46741 gtcgataaag ccttttaaca tgccgcgtac ctgcatgaac tccagcggcg ggcagccagc
    46801 ggatagcggg tcaaactggc gaattagtgc atcaagctgg ctggcgatga gcggttcgct
    46861 aatcggcagg taaaactcca tctccacctg tttatcgcgg tcggaaagct gactcaggct
    46921 aacacccgtt tcattgagag gtgcctggag gacagccgtg atccactcgg tcaacaccgg
    46981 ttcccactgc gattcaaagc cgccgagttc cagtttttcc tgtacccagt tcgggtcaac
    47041 cggctgggta aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc
    47101 gcgcgggaac tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc
    47161 atcaacatcc agccgtggca tcagatcctg agcgataccg tgaccacgct gctgcaagcc
    47221 ggagtagctg gtgacacgcc agttatcgcc gggcaatcgt tgtagtgtcc tcgcattcag
    47281 ttctgcagtt aaagcatcat taacctgcca gggctggtta tcaccagttt gtgccgtttg
    47341 ccaggcaata tcatcatcgc ataacgcttc aatacaggtg cgaagccctg ccgcatcttg
    47401 cggttcccct ttttgcagca aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt
    47461 tttatcgcca cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg
    47521 cgtcagcgcc acgtaaagca aacgcagatc ttccgccaga cgttcgacct ccgcgaggtc
    47581 gacgctttct ggcgcagcgt taagatccaa aactgcctca aacgagtggc gatcgtgata
    47641 aaacgcctga tcctggacgc ggaaattggt gataaacggc agccagacca atggatattc
    47701 cagccctttc gatttgtgga tcgtgacaat ctgcaccaga tgtttatcac tttcgagacg
    47761 catttgttgg ctggaggcat tactgtctgg ctcgaggata tgttgcgata accagcgtac
    47821 cagcgcatgt tcactttcca gctgcgttcc ggcttcttgt agcagttcgc tgatatgcaa
    47881 gatatcggta agacgccgct caccgcctgc cgttgccagc aagttttcag caatgttacg
    47941 cgccgacatc agcgcccgca gcatcggcat aacgccacgt ttgcgccaga tttgccgata
    48001 accatcgaac tcttcgacta ccacatccca cgcatgttcg tcattgttca gcgtttcgat
    48061 atccagcgcg ttcagcccca tcattgacgt tgccagcgca ctacgcaggg tgttctcacg
    48121 ttcgggcgtc atcaccgcct gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa
    48181 aacactgtcg cggttcgaaa ggtaaacgga agggatttcc agcaacgtta aggcgtcgcg
    48241 cacctgggcg gcctcctggc ggctgcgcac cagcacactg atgtccgaag cacgcaccgg
    48301 acgcgcgtcg tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc
    48361 gcggatttgc gcagcacata cctgcgccat ggtactttga tagtcgccaa cgccgcagct
    48421 ttcgccttcc atcagccaca ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg
    48481 taacgcctga tttttcccgg ctgatttcac tggaataaac ggtatttcgc gaaacatgaa
    48541 cgcgtcatca gtctggctga aaagcttgtt cacactgttc accattcctg gtgcggaacg
    48601 ccagttggtg tctaaagtgt agtgggcgtg aacttcgcta cgcgccttca tataagtgaa
    48661 gatatccgca ccccggaatg catatatggc ctgtttcggg tcgccaatca gcaacaatgc
    48721 ggtttccggc tgatggtgcc agatacggcg aaaaattcgg tactgctggg ggtcggtatc
    48781 ctgaaattca tcaatcattg ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc
    48841 gccgctttcg ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa aacccaattc
    48901 gccacggcgg cgtttttcac gcgctactgt ttcgcggatc tcagccaatg cgcgggttat
    48961 caccagatca cggatcgaca atggttctgc aagcagttgt tcgatcgcct caaacagtgg
    49021 atgtcgcggg gtttcccccc cggctttcgt gcgatcttct aagaaacgct gagagaattt
    49081 ttccagcgac tccggtaact gataactgtt ggtctcttct tctgcccagg cgctgatctt
    49141 ctcgatccat ttagcctgat tgctacggtt aaacttgcgt cgatcaatac cagaagattc
    49201 gatcagcgca tccagttcac ccactgcgtc gcgccactgc tgttttaccg tatcaatacg
    49261 cgccacaatt tgcgcgtggc gggaagccag cgtttcatca tcgggcggcg gtgctttgat
    49321 aaccggtgct tcgccttgca gataacgatt aatatcgcgc agcaacgcct gcggcccttt
    49381 ccaggtttca aagaccacca gggcaatttc acgcggcagc gggtagcagt ggcgacgcca
    49441 gaaatcggcg caggcctggt agcgtagcag agactcatct tcaatcagct gctgctcaaa
    49501 cagcataccg gattcaaagg cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt
    49561 aaagactgcc gcttcatcca tctgccgttc ggctaacaac aaccactgcg cggcttgcgc
    49621 tttatcgtcg atctcttcca gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag
    49681 acaggcgatg cgcaactcgt ggatattgct acggatacga ccgcgcaatt ctgccgtggc
    49741 agcctcggta aaggtgacca ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc
    49801 ggaaccgcct agtccaagta acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc
    49861 tgtgccggca gaggcttcaa tcaggcgctc gccctgtaag ggcaagcgca aaggatctag
    49921 tgtctcggcg acatcactca ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc
    49981 tgacgttctc ccacactttc cagccttcag gatgcacata ttcggctttc ccgttctggc
    50041 tgccggaaat ctgcgacagg atagccatgc cttgcggctc gaccaccgcc tgatggaaga
    50101 aatcagcaag tttttgcggc gtcagcagtt ttatctgggc cacgatttta tcacgcgaat
    50161 cgaagcgcat attgccgcga tcgaaatctt tacttaactt cgatgcttct tcgccgagcg
    50221 tttgcggtgc ctgcagcatc tgggtaatta ccgcctgctg gatttgcgca aactcatctg
    50281 gcttcatcgc tcgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca
    50341 agaatgaagg ctgtttatca ttgctttgca aaaggaagcc catgccccac tgacgcccca
    50401 cgctcattgg aaacgcaaac acggcatagc ccaattgttc ttcggtacgc aactgattgt
    50461 agaaccacgg ctgtacgatc tgccccaaca gagagctata ggctgagctg gtgtattcat
    50521 cgtagccagt cggtacaaat accgctgcca gtgcggagtc ggtgctgtta ccggcttttt
    50581 caaagatgac ggattgtttt ttatcgacca ctacatcttt gtttcgacac cactctgaac
    50641 catcagcgcc caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg gcctcggtca
    50701 tgttgccgat aaccataaac tctggtcgag cccctgattt taaggcgtcg cgataggcca
    50761 gcacctcttt caacgtaatg gagggcaaaa ttttacgccg ttcatctcgc gagaagtacg
    50821 gcacttgcga gagcatctgt gcgggcataa tcgcctgctc aaacgcttta cccttttctg
    50881 cggaatccat catctggtta taccaggact tcgcctgctc aagctgatct tccgtagcgg
    50941 tatagctaaa gtacccctcg agcaatgcct ggaacagctg cggcagacgc tgggtgtaac
    51001 cattagcatt aaccataagg ccgttgttag cgttggtgga aaaacttatg ccaccaaccg
    51061 acgcctggtt gcttaactga tcaagcgcca gccctgcgag ataatcattg agcgcaaaca
    51121 tcacctgatt gcgggcgctg tccatggctt tcggattacg caaaatcagg ctgacatcag
    51181 ctttgggttc gctggaaaaa taacggcttg gcgcatacac cacgcgcaga ttcgactcat
    51241 caacaatcag ctctggatgg tcatatttct tctctgactt aatcagcgag aaatcgtcag
    51301 gaatataggg gttaagctcc ggtaaggaga gcgcaatatt ggcagctttt tgctgccagt
    51361 cagcgaaagt ttgttcgctg attttatcga cctgatacgg cgcatcgaca aaataagccg
    51421 ttttattgtg cggctctttc gggctgatat accagatacg cgcattctgc ggcgtcatca
    51481 tcgccagacg ttcttttact gctttagcat cgtaccgatc ggcaatattg actgcatcca
    51541 gcgtatgctc aaccggaacg cgaatcatgg tatctgccag ccattcgacg taatccatat
    51601 cacgggtgat cgacggataa cggaagtcga tatccagcac attcgccagt tcatcgaagt
    51661 attgtttatc aatgcctttt tcacgtaaca gattgagata gctaaaaatt gccgccacaa
    51721 cctgatcgcg attagccagg cctttatcgg ttaaagacgc agagatcgct aatacgccgc
    51781 tgttgccgtt gacgatagga tcggagttgg cgctaatgcc ctcaactaat ccctgctttt
    51841 gcagccagtc ggaaagtgta cctgggctgc gattaccaat cagataggta atcaattcat
    51901 ccgttttact acggaacttc gctgagttgt tatcgatgcg aaactcaacg cgcagaactt
    51961 tacgcggcag tgccgggacg taatgaatga taatgccctt ttgcgcgtcg gtgactaccg
    52021 gcacggtgat ttccggtttt ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg
    52081 ccatttttgc caactccggc agcggtttat tgctgtaaat aaccgccttc atcagattgg
    52141 cggaatagta cttctcgtgg aaatctttca gcgcctgctg caccggatta ccaggtttat
    52201 cgcttaaagt ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg
    52261 tttctgcgct gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg gttaattcag
    52321 cgttcaccgc attacgctca cgttcggcat atttcttgtc gagcaaaggt tcagcaatag
    52381 catcggccag gcggtctacc gcaccaggca aggcgtcgtt ctcaacttcc agatagaaag
    52441 ccgtgcgata cggcgcagtg ctggcattgt gactaccgcc gtgcattttg agatattcgg
    52501 ccagactgtc agcctgcggg tactttttcg accccatcag actcatatgt tcaaggtaat
    52561 gtgccagccc ctggtacgcc tcgggatctt ccagcgaccc aacgggcacc accagcgccg
    52621 agagcgattt aactgcctgc ggatcagaaa ccagcaagac caccataccg ttatccagac
    52681 gtatagcctg atactggcgg ttatctttat cacttttacg gatggtttcc tgaatcggct
    52741 gccatcccgt ttctgcctga cttaagggtg cccaaagggc aacaaacaac aataatgctt
    52801 tgaaccaggt gctgcggggc attcacggac ctcataatca actttatttt ctggccagat
    52861 tcaacaggtt aacgcaaatc atctgccaga atttaatctt gtgctgcacg agtcagccta
    52921 tgtctatata tccatcagtc cgtgactggt gcgcatcata aagtaagcgg atagattgcg
    52981 caatttttat acagcactca tgactgatta aagcgaaaca gcggtaacag gaaacgttgc
    53041 gactgttcaa cgatggcctc cattgtctct ggtgttaatt gccgccagag cctttgatac
    53101 cagatatcat caccttcgcc acgcaccatc atgttgcctt cgtaagcctg aaggaatttc
    53161 gtacgggctt tttgcaacgt ggaatcgtca tccagcatgg catcgttttg cgcgtcataa
    53221 caggttttta gccacgcgcc gccactttca ggtaacacca gcaatggcgc ggacatccct
    53281 tcacgatacc cctcaatcag ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc
    53341 ggaaaacgcc actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt accaccgctg
    53401 gcacagtaga caaggtgttc cagccaaagt tgcattccct gcgccacact taataaagag
    53461 ggacgccagc gcaacaggcc atccggctgc acctgcggca accagccagt tatctgcaca
    53521 ccgttgcagg cgagatcaat ttccatactc tgccccggct ggcgacaggc aatgactctg
    53581 tcggcaagct gctgcatctc ctggcactgt gtttcccaga aaatttcacc aaaagcgcca
    53641 tacggtaaat cccctgccgc ccggaagcgg cggaacaagc gttcggcatc atcctgctca
    53701 accagtgcat tcaataactg ctgattgatt tgataacggc taagcccttc cagaataaat
    53761 ggctcggtgt ctgggatttc gctgtcttca gtacggaagt tcacctgcaa acgcatctgg
    53821 aagaacgccc gcaccggatg tgcccagaat cgttgtagcg tttccagcgg cacggtttcc
    53881 ggtaaggtaa acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc
    53941 gcaggtagcc attcacgagc atagctttgt cgttcgcctg gctggtagtt ttgcggatca
    54001 aacggcatcc gggtatggag gcaagtaaga tgcgctttta cccttgcctc gctttcatca
    54061 cagttgagcg cttcatcgcc cggtagataa tgactttgcc cgatgtagtc gatcagttcc
    54121 tgcaccagta ccgacgggaa acgttcactg ttatcctgaa tggaacgccc gatatagctg
    54181 atatagagtt tttgctgcgc ggaaattaac gcttccagga acaggtagcg gtcgtcatcg
    54241 cgacggctac ggtcgccacg cttcggtttc tggctcatca ggtcaaagcc caatggcgca
    54301 agctgacgtg gataaacgcc gtcgttcatt cccagcaggc aaaccacttt gaacggaatt
    54361 gaacgcattg gcatcagagt acaaatgtta accggtccgg cgagaaaacg ctggctgata
    54421 cgttcttgat ccagacgctg cgccagttca tcacgcaata gtgacagcgg caccgcgtcg
    54481 ccatactgcg cacctaaacc ttcggcgata atcgcctgcc attgttgttc gatcagcgtc
    54541 atcgccgctt cggtttccgc atccggcagg aagaaggcgt tgagcatatc gcgacaaacc
    54601 ggcaaccact cttctagcgg acgctcctgt gccagcccgc gacgccagat gtttagctgc
    54661 attagcagtg aagccagatg ccccaccagt tctgcaatta agccgctcga ttcatcataa
    54721 ggtagaaccg attgccactc gccctgcgcg ctctccatcg cgtagcccag caacatacgc
    54781 gtcaggccaa atcgccaggt gtgttgtccg gtggtgggga gttccagctc gcgaacgttg
    54841 tcgtcatcta tcccccaacg aatgccggat tcgttgaccc actggcgtaa ataacgcagc
    54901 ccttcttcgg tgatgtcaaa ccgcgccgcc agcaccggca catccagtaa tgccagcacg
    54961 tcttccgaca caaagcggct gtctggcaac gataacaggc tgataaacgc ttccagtacc
    55021 ggatgcgact gccgcgcccg acggtcggaa atggcgtaag gcaggtaacg atccgcaggt
    55081 gcgctaccaa acacagcctg aataaacgga ctgtagctgt cgatatcagc caccatcacg
    55141 atgatgtcgc gcggagtaag tgtcgggtct tcttccagca tcgccagcag gcgatcgtgc
    55201 aaaacttcaa cttcacgctg cgggctatgg caaacgtgga aggtgatact gctatccagt
    55261 ggatcaagcg ggcgtttgtt atcgctacgg gaaaactctt cgatgttcac accagcaacg
    55321 gcacggtttt ccagttccag aatgtcagac tgaatgttat gcagcaggtt atctggcgtg
    55381 acatcgacaa aggcgtccag ctcctggctg ctctccaggt cagaaaggag ataaatgtag
    55441 tcgcgcccaa gcttacccca tgaagccagc agcgggttgc cgacatcctg ttcaccatcg
    55501 ctgttaaaga gctgcccggc attttcgcta tcacgaaata gcggcaattc gcgatcttca
    55561 aaactgtgtc gacgctgacg ggtcagcagt ttcgccagat aagcaggatc tttaatatcg
    55621 ccccagtaat aacggcaggg gttggtaaac aggagatgga tttcaatatg tttacccagc
    55681 gcctggagcg cctggagata aacaggcggt aacgcggaaa taccgcatat aaagacgcgc
    55741 gaaggtaacc ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag
    55801 agattggcgc ggtgccaacg cggttgtccg agttcatggg tatattccac cagcgccttc
    55861 cacaacggcg cttgccaggc ctgtgcttct cccagccctt caaccaaatg tcctgtttcc
    55921 cactgtgcca gccactccgg gcgatagacc agatactggt caaacaggtc cgccgctttt
    55981 gaggaaagct ggaacagttt tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg
    56041 gtaaagtctt cgcgatccag caattgcggc agcagagtca tcagtttcca gctcatgctc
    56101 tgtttgttaa aggcgctctc tttggggatc tccggtaaca cccggacgaa catatcccag
    56161 ataaagctcg ctggcagcgg aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg
    56221 gtcatttgca gccactgcgc cataccggta ctttgcacca gaatcatctc tggttcgaaa
    56281 ggatcgtcca gccgttcgcg ttcgacaata aactccatca acgcttccag cacgtccaga
    56341 cgattggaat ggtagaccct taacatagcg gctcctgact actgacgatt cgggcaatgc
    56401 aggcgggtca tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca
    56461 catcccgcct gcgatgtctg cattcggttg acctgccagt tggcaggtgg cgaaatcgcg
    56521 cgcagttgcg tttgctgcca accatgccgc cagagctgtt ggtactggtt tctgctggcg
    56581 agactgttca ttaatgttcg ctggataccc gataacgcag tgacaatcat caccatcagc
    56641 accatcgcaa ccattacctc cggcaggcta aagccttgtt gattcttcag ggaagctgac
    56701 ataacgccct ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaacaatgt
    56761 ttccatcaat gacttcgcct gttcgccaca acgaaacgcc ttcataaccg gcaaccaata
    56821 aggcttcatt atctgccagt aaacgcaaac aaacccgggc atcggttcca gcgtactgcg
    56881 agcactgaac tgcgggctgc gtctgccagg agtgcatttt cccccacgcc agcgccgact
    56941 gaacgatggc ctggcggcgc aatgactgac tttccatgct cacgcgagag gcaaaactgc
    57001 gatcctgctg actcattcct tgcaatagca agctacccaa aaccagcagc atcagaacca
    57061 gagccagtga cgaaacacct ttttcgcggt tcacaggttg aatcctgtca cgctatagct
    57121 ggcatccacc acggtttgcg gttcagactt actggcagca cgcatattaa ccgtcaacac
    57181 cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc
    57241 cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc
    57301 cagcacatgc tccttcagac gaaatccaat ctggtcagac tcttttaccg gttcgcgatc
    57361 ccagataccg ttactgttcg catcccactg cacaatgata cagtcaccct gtccgacaat
    57421 ttccagccct tcgccggtac agctgccatg acaataaccc gccctctgga gatgcttcgc
    57481 gacggtaaat acccgcagcc agatttcatc ttccagcgcc agcttacggg tgctcgttaa
    57541 actttcacgc tgtaacgcag gcagaaagcg tgcagccccc agcaacaata cgctactgat
    57601 cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct
    57661 gtttctcctt gctgacaaag ccggagtctt ccccacggcg aaaccaccag ccaccactcg
    57721 cccgttgagt ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa
    57781 gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac aaagaccaat
    57841 ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca ccataacgtc
    57901 ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa
    57961 tagagcaaat agtcccgcgc ctggctggcg gtttgccata gccgttgcga ctgctgccag
    58021 tattgccagc catagagtcc acttgcgctt agcatgacca aaatcagcat cgcgaccagc
    58081 gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa
    58141 aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt acgccgttgc
    58201 aggacgttgt aaaatttcgg gaaggcgtct cgaagaattt aacggagggt aaaaaaaacc
    58261 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    58321 aatgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    58381 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    58441 cagatgagtt tgatccatat gattgctgta caggtgcgtg tcgccccccg tccagacaaa
    58501 atcgcccact tccagatcac actgctgcgc catcatatgc accagtaacg cgtagctggc
    58561 aatgttaaac ggcaggccga ggaaaacgtc acaggagcgc tgataaagct ggcaagagag
    58621 tttgccgtct gccacataga actggaagaa cgcatggcac ggtgccagcg ccattttatc
    58681 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    58741 ctggttcagt accgtagtga tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    58801 ccactgttta ccatacactg gtccgaggtc gccgttttca tcggcccatt cgtcccagat
    58861 ggtgacattg ttttcgtgta gataagcaat gttagtgtcg ccctgcagaa accacagcag
    58921 ttcgtggatg atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg
    58981 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    59041 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    59101 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    59161 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    59221 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    59281 caggaacaag ccagagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    59341 aataaacagc accacacctt ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag
    59401 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    59461 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    59521 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    59581 tgcgataaaa tcagaaacct ggaagaagga acgtttagta cggcgggcaa agataatcat
    59641 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    59701 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    59761 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    59821 ccagccgctg cccggacgat tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc
    59881 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    59941 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    60001 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    60061 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    60121 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    60181 aacctgatgg cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    60241 tgcttcggca taatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    60301 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    60361 catttcaccg cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc
    60421 cagagctcgt aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt
    60481 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    60541 ccgctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    60601 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    60661 caggcggcgt gcttcatcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag
    60721 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    60781 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    60841 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    60901 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    60961 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacggctgcc
    61021 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    61081 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    61141 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    61201 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    61261 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    61321 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    61381 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    61441 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt
    61501 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    61561 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    61621 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc
    61681 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    61741 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    61801 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    61861 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    61921 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    61981 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    62041 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    62101 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    62161 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    62221 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    62281 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    62341 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    62401 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    62461 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    62521 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    62581 attgatttct gcatcgccgc tcaccagctg caagagaaac catttttgtt tttggccgat
    62641 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    62701 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    62761 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    62821 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    62881 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    62941 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    63001 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    63061 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    63121 tacgggaaaa cccggagcat tctgaataag cccgatatag
//
LOCUS       DAPHDX010000005        62906 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPHDX010000005 DAPHDX010000000
VERSION     DAPHDX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.005"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(104..180)
                     /locus_tag="RH058_000341"
     tRNA            complement(104..180)
                     /locus_tag="RH058_000341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Arg,seq:acg)
     gene            complement(378..454)
                     /locus_tag="RH058_000342"
     tRNA            complement(378..454)
                     /locus_tag="RH058_000342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Arg,seq:acg)
     gene            complement(458..550)
                     /locus_tag="RH058_000343"
     tRNA            complement(458..550)
                     /locus_tag="RH058_000343"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(514..516),aa:Ser,seq:gct)
     gene            complement(866..1051)
                     /gene="csrA"
                     /locus_tag="RH058_000344"
     CDS             complement(866..1051)
                     /gene="csrA"
                     /locus_tag="RH058_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV3135985.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1286..3916)
                     /gene="alaS"
                     /locus_tag="RH058_000345"
     CDS             complement(1286..3916)
                     /gene="alaS"
                     /locus_tag="RH058_000345"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV3135986.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4044..4544)
                     /gene="recX"
                     /locus_tag="RH058_000346"
     CDS             complement(4044..4544)
                     /gene="recX"
                     /locus_tag="RH058_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV3135987.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4787..5848)
                     /gene="recA"
                     /locus_tag="RH058_000347"
     CDS             complement(4787..5848)
                     /gene="recA"
                     /locus_tag="RH058_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV3135988.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5928..6425)
                     /gene="pncC"
                     /locus_tag="RH058_000348"
     CDS             complement(5928..6425)
                     /gene="pncC"
                     /locus_tag="RH058_000348"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV3135989.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6570..7655)
                     /gene="mltB"
                     /locus_tag="RH058_000349"
     CDS             complement(6570..7655)
                     /gene="mltB"
                     /locus_tag="RH058_000349"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV3135990.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7911..8474
                     /gene="srlA"
                     /locus_tag="RH058_000350"
                     /gene_synonym="gutA"
     CDS             7911..8474
                     /gene="srlA"
                     /locus_tag="RH058_000350"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV3135991.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8471..9430
                     /gene="srlE"
                     /locus_tag="RH058_000351"
                     /gene_synonym="gutE"
     CDS             8471..9430
                     /gene="srlE"
                     /locus_tag="RH058_000351"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV3135992.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9441..9812
                     /gene="srlB"
                     /locus_tag="RH058_000352"
                     /gene_synonym="gutB"
     CDS             9441..9812
                     /gene="srlB"
                     /locus_tag="RH058_000352"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV3135993.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9816..10595
                     /gene="srlD"
                     /locus_tag="RH058_000353"
     CDS             9816..10595
                     /gene="srlD"
                     /locus_tag="RH058_000353"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV3135994.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10701..11060
                     /gene="gutM"
                     /locus_tag="RH058_000354"
     CDS             10701..11060
                     /gene="gutM"
                     /locus_tag="RH058_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV3135995.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11127..11900
                     /gene="srlR"
                     /locus_tag="RH058_000355"
                     /gene_synonym="gutR"
     CDS             11127..11900
                     /gene="srlR"
                     /locus_tag="RH058_000355"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV3135996.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11893..12858
                     /gene="gutQ"
                     /locus_tag="RH058_000356"
     CDS             11893..12858
                     /gene="gutQ"
                     /locus_tag="RH058_000356"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV3135997.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSNALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12855..14369)
                     /gene="norR"
                     /locus_tag="RH058_000357"
     CDS             complement(12855..14369)
                     /gene="norR"
                     /locus_tag="RH058_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV3135998.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14556..15995
                     /gene="norV"
                     /locus_tag="RH058_000358"
     CDS             14556..15995
                     /gene="norV"
                     /locus_tag="RH058_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV3135999.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15992..17125
                     /gene="norW"
                     /locus_tag="RH058_000359"
     CDS             15992..17125
                     /gene="norW"
                     /locus_tag="RH058_000359"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV3136000.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17351..19603)
                     /gene="hypF"
                     /locus_tag="RH058_000360"
     CDS             complement(17351..19603)
                     /gene="hypF"
                     /locus_tag="RH058_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV3136001.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSTAVATLRARKHRPAKPLAVILPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19756..20283)
                     /gene="hydN"
                     /locus_tag="RH058_000361"
     CDS             complement(19756..20283)
                     /gene="hydN"
                     /locus_tag="RH058_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV3136002.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20432..21442)
                     /gene="ascG"
                     /locus_tag="RH058_000362"
     CDS             complement(20432..21442)
                     /gene="ascG"
                     /locus_tag="RH058_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV3136003.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21702..23159
                     /gene="ascF"
                     /locus_tag="RH058_000363"
     CDS             21702..23159
                     /gene="ascF"
                     /locus_tag="RH058_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV3136004.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            23168..24592
                     /gene="ascB"
                     /locus_tag="RH058_000364"
     CDS             23168..24592
                     /gene="ascB"
                     /locus_tag="RH058_000364"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV3136005.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24751..25221)
                     /gene="hycI"
                     /locus_tag="RH058_000365"
     CDS             complement(24751..25221)
                     /gene="hycI"
                     /locus_tag="RH058_000365"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV3136006.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(25214..25624)
                     /gene="hycH"
                     /locus_tag="RH058_000366"
     CDS             complement(25214..25624)
                     /gene="hycH"
                     /locus_tag="RH058_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV3136007.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25621..26388)
                     /gene="hycG"
                     /locus_tag="RH058_000367"
     CDS             complement(25621..26388)
                     /gene="hycG"
                     /locus_tag="RH058_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV3136008.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26388..26930)
                     /gene="hycF"
                     /locus_tag="RH058_000368"
     CDS             complement(26388..26930)
                     /gene="hycF"
                     /locus_tag="RH058_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV3136009.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVEADLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26940..28649)
                     /gene="hycE"
                     /locus_tag="RH058_000369"
     CDS             complement(26940..28649)
                     /gene="hycE"
                     /locus_tag="RH058_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV3136010.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28667..29590)
                     /gene="hycD"
                     /locus_tag="RH058_000370"
     CDS             complement(28667..29590)
                     /gene="hycD"
                     /locus_tag="RH058_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV3136011.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29593..31419)
                     /gene="hycC"
                     /locus_tag="RH058_000371"
     CDS             complement(29593..31419)
                     /gene="hycC"
                     /locus_tag="RH058_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV3136012.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31416..32027)
                     /gene="hycB"
                     /locus_tag="RH058_000372"
     CDS             complement(31416..32027)
                     /gene="hycB"
                     /locus_tag="RH058_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV3136013.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32152..32613)
                     /gene="hycA"
                     /locus_tag="RH058_000373"
     CDS             complement(32152..32613)
                     /gene="hycA"
                     /locus_tag="RH058_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV3136014.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32825..33175
                     /gene="hypA"
                     /locus_tag="RH058_000374"
     CDS             32825..33175
                     /gene="hypA"
                     /locus_tag="RH058_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV3136015.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33179..34051
                     /gene="hypB"
                     /locus_tag="RH058_000375"
     CDS             33179..34051
                     /gene="hypB"
                     /locus_tag="RH058_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV3136016.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34042..34314
                     /gene="hypC"
                     /locus_tag="RH058_000376"
     CDS             34042..34314
                     /gene="hypC"
                     /locus_tag="RH058_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV3136017.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34314..35435
                     /gene="hypD"
                     /locus_tag="RH058_000377"
     CDS             34314..35435
                     /gene="hypD"
                     /locus_tag="RH058_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV3136018.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35432..36442
                     /gene="hypE"
                     /locus_tag="RH058_000378"
     CDS             35432..36442
                     /gene="hypE"
                     /locus_tag="RH058_000378"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV3136019.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGTTILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36516..38594
                     /gene="flhA"
                     /locus_tag="RH058_000379"
     CDS             36516..38594
                     /gene="flhA"
                     /locus_tag="RH058_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV3136020.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(38631..38984)
                     /locus_tag="RH058_000380"
     CDS             complement(38631..38984)
                     /locus_tag="RH058_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV3136021.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(39061..39195)
                     /locus_tag="RH058_000381"
     CDS             complement(39061..39195)
                     /locus_tag="RH058_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136022.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            39271..41832
                     /gene="mutS"
                     /locus_tag="RH058_000382"
     CDS             39271..41832
                     /gene="mutS"
                     /locus_tag="RH058_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV3136023.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41938..42594
                     /gene="pphB"
                     /locus_tag="RH058_000383"
     CDS             41938..42594
                     /gene="pphB"
                     /locus_tag="RH058_000383"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV3136024.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(42645..43412)
                     /locus_tag="RH058_000384"
     CDS             complement(42645..43412)
                     /locus_tag="RH058_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3136025.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            43608..44516
                     /locus_tag="RH058_000385"
     CDS             43608..44516
                     /locus_tag="RH058_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV3136026.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            44513..45775
                     /locus_tag="RH058_000386"
     CDS             44513..45775
                     /locus_tag="RH058_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDV3136027.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45772..46410
                     /locus_tag="RH058_000387"
     CDS             45772..46410
                     /locus_tag="RH058_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3136028.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            46415..47191
                     /locus_tag="RH058_000388"
     CDS             46415..47191
                     /locus_tag="RH058_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV3136029.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47280..48644
                     /locus_tag="RH058_000389"
     CDS             47280..48644
                     /locus_tag="RH058_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV3136030.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(48683..48919)
                     /locus_tag="RH058_000390"
     CDS             complement(48683..48919)
                     /locus_tag="RH058_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136031.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(48930..50357)
                     /locus_tag="RH058_000391"
                     /pseudo
     CDS             complement(48930..50357)
                     /locus_tag="RH058_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(50357..50950)
                     /locus_tag="RH058_000392"
     CDS             complement(50357..50950)
                     /locus_tag="RH058_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDV3136032.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51097..51504
                     /locus_tag="RH058_000393"
     CDS             51097..51504
                     /locus_tag="RH058_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV3136033.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(51625..52617)
                     /gene="rpoS"
                     /locus_tag="RH058_000394"
     CDS             complement(51625..52617)
                     /gene="rpoS"
                     /locus_tag="RH058_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV3136034.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(52680..53819)
                     /gene="nlpD"
                     /locus_tag="RH058_000395"
     CDS             complement(52680..53819)
                     /gene="nlpD"
                     /locus_tag="RH058_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV3136035.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(53959..54585)
                     /gene="pcm"
                     /locus_tag="RH058_000396"
     CDS             complement(53959..54585)
                     /gene="pcm"
                     /locus_tag="RH058_000396"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV3136036.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(54579..55340)
                     /gene="surE"
                     /locus_tag="RH058_000397"
     CDS             complement(54579..55340)
                     /gene="surE"
                     /locus_tag="RH058_000397"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV3136037.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(55321..56370)
                     /gene="truD"
                     /locus_tag="RH058_000398"
     CDS             complement(55321..56370)
                     /gene="truD"
                     /locus_tag="RH058_000398"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV3136038.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(56367..56846)
                     /gene="ispF"
                     /locus_tag="RH058_000399"
     CDS             complement(56367..56846)
                     /gene="ispF"
                     /locus_tag="RH058_000399"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV3136039.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(56846..57556)
                     /gene="ispD"
                     /locus_tag="RH058_000400"
     CDS             complement(56846..57556)
                     /gene="ispD"
                     /locus_tag="RH058_000400"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV3136040.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(57575..57886)
                     /gene="ftsB"
                     /locus_tag="RH058_000401"
     CDS             complement(57575..57886)
                     /gene="ftsB"
                     /locus_tag="RH058_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV3136041.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58080..58403)
                     /locus_tag="RH058_000402"
     CDS             complement(58080..58403)
                     /locus_tag="RH058_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV3136042.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(58453..59058)
                     /gene="cysC"
                     /locus_tag="RH058_000403"
     CDS             complement(58453..59058)
                     /gene="cysC"
                     /locus_tag="RH058_000403"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV3136043.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59058..60485)
                     /gene="cysN"
                     /locus_tag="RH058_000404"
     CDS             complement(59058..60485)
                     /gene="cysN"
                     /locus_tag="RH058_000404"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV3136044.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(60487..61395)
                     /gene="cysD"
                     /locus_tag="RH058_000405"
     CDS             complement(60487..61395)
                     /gene="cysD"
                     /locus_tag="RH058_000405"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV3136045.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            61647..62684
                     /gene="iap"
                     /locus_tag="RH058_000406"
     CDS             61647..62684
                     /gene="iap"
                     /locus_tag="RH058_000406"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV3136046.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        14743 a        17233 c        16358 g        14572 t
ORIGIN      
        1 ctcgcccttc gggccgttgc taaagcaacg ttatcctccc tggtgcttgc ggttagtacc
       61 gccacctctt cgataccttc atcgctgaag attacggaga atatggtgca tccgggagga
      121 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
      181 atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg tatcaagtaa
      241 gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa
      301 gcaacgttat cctccctggt gcctgcggtt ataccgccac ctcttccata ccttcatcgc
      361 tgaatgtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
      421 agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa
      481 ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc agcctctcgg
      541 ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc
      601 tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc ctacactttt
      661 atcgtttgca cacttcacgt tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca
      721 acagaaaaat ggaggtctga cagatagtag taatgcaaaa aaatggagac ttaagttgaa
      781 tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg gtgcgtctca
      841 ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt tcagcctgga
      901 tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt acgccaatac
      961 gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca atcatgaggg
     1021 tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat taaaagagtc
     1081 gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag caatgacaaa
     1141 cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt agccgatata
     1201 cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag
     1261 tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag cctttcacac
     1321 tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac
     1381 gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc cctgctttca
     1441 cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct tcggctaccg
     1501 ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc atggtacgca
     1561 acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca ttaacatcaa
     1621 ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta agctgttgta
     1681 gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc gccagattat
     1741 tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg
     1801 tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca gcagtacccg
     1861 attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga gtaccgccac
     1921 acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca tacttctcgc
     1981 cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg ttggtttcga
     2041 tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga atctcttccg
     2101 gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta accagtgaac
     2161 ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg
     2221 cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt
     2281 cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc tggccgtatt
     2341 tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct ttatcaccaa
     2401 cctgaccgcc ggattccgca tagaatggtg tttgatccag cacgaccaca gcttcctggc
     2461 ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact ttgccgttca
     2521 gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc attgcgttgt
     2581 aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc attgctgctt
     2641 caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa
     2701 ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc gtatcaccag
     2761 aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta cgggcaaact
     2821 gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac
     2881 ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg
     2941 cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac
     3001 ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt
     3061 cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact ttcgctaccg
     3121 cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg
     3181 cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc ggttccatcg
     3241 tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca atgtagcggt
     3301 cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg tgatcgtaga
     3361 agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag ttgtcagatg
     3421 cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg atcccgactt
     3481 ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc cacagacgct
     3541 ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat
     3601 cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag gtatggtgac
     3661 gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt
     3721 gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca tccttgaact
     3781 ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc agggagctgc
     3841 tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga cggatctcag
     3901 cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc accagctcca
     3961 gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt tttcttgact
     4021 gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc ctggatatct
     4081 tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc tgaaaagaca
     4141 gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg
     4201 atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt ctgattcagt
     4261 tcctggcgaa tacgcgcagg tccataacct ttgcggctac ggctggcgat aaagcgcgca
     4321 acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac gcgctcgtaa
     4381 tcttctgccg tagcgtcaat ctcttctggg ccatttttgc ccataatcgg tgccgcgagt
     4441 ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc
     4501 aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata tccttacaac
     4561 ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca gatttaatag
     4621 tcaatgagtg tgcagaatgt tcccgcaggc ccaattcgtt agaggatcga ggtgagagta
     4681 atttttcacg ccagtgctgg cctgttcccc tcaccctaac cctctcccca aaggagcgag
     4741 gggaccgtcc gtgcacacat tatctattat catcaataac gaacgattaa aaatcttcgt
     4801 tagtttctgc tacgccttcg ctgtcatcta cagagaaatc cggcgttgag ttcgggttgc
     4861 tcagcagcaa ctcacgtact ttcttctcga tctctttcgc ggtttccggg ttatctttca
     4921 gccaggcagt tgcattcgct ttaccctgac cgatcttctc acctttgtag ctgtaccacg
     4981 cgcctgcttt ctcgatcagc ttctctttta cgcccaggtc aaccagttcg ccgtagaagt
     5041 tgataccttc gccgtagagg atctggaatt cagcctgttt aaacggcgca gcgattttgt
     5101 tcttcaccac tttcacgcgg gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc
     5161 cgatacgacg gatgtcgaga cgaacagagg cgtagaattt cagcgcgtta ccaccggtag
     5221 tggtttccgg gttaccgaac atcacaccaa ttttcatacg gatctggttg atgaagatca
     5281 gcagcgtgtt ggactgcttc aggttacccg ccagcttacg catcgcctgg ctcatcatac
     5341 gtgccgcaag gcccatgtga gagtcgccga tttcgccttc gatttccgct ttcggcgtca
     5401 gtgccgccac ggagtcaacg acgataacgt ctactgcgcc agaacgcgcc agggcgtcac
     5461 agatttccag tgcctgctcg ccggtgtccg gctgggagca cagcaggtta tcgatatcga
     5521 cgcccagttt acgtgcgtag attgggtcca gcgcgtgttc agcatcgata aacgcacagg
     5581 ttttaccttc acgctgcgct gcggcgatca cctgcagcgt cagcgtggtt ttaccggaag
     5641 attccggtcc gtagatttcg acgatacggc ccatcggcag accacctgcc ccaagcgcga
     5701 tatccagtga aagcgaaccg gtagagatgg tttccacatc catggaacgg tcttcaccca
     5761 ggcgcatgat ggagccttta ccaaattgtt tctcaatctg gcccagtgct gccgccaacg
     5821 ctttctgttt gttttcgtcg atagccattt ttactcctgt catgccgggt aataccggat
     5881 agtcaatatg ttctgttgaa gcaattatac tgtatgctca tacagtatca agtgttttgt
     5941 agaaattgtt gccacaaggt ctgcaatgca tacgcagtag cctgacgacg caccgcatca
     6001 cggtcgccgc tgaagcattc ccgccgggta atgccttcac cgcgggcagt ggcaaaagca
     6061 aaccagacgg tgccgacagg cttctcttca ctgccgccat ccggcccggc gataccacta
     6121 atagacacgg cataatcagc acgagccgct ttcagtgcgc ctatcgccat ttccaccacg
     6181 acgggttcac tcaccgcgcc atgctgcgcc agcgtctctt cgcgtacgcc gatcatctgc
     6241 gctttggctt cgttactgta ggtgacaaat ccgcgttcaa accaggcgga gctaccggca
     6301 atatcggtaa tcactttcgc tacccaacca ccggtacaag actcggcagt tgttacggtt
     6361 gcgccacggg ctttcagcgc ctgcccaacc tgttcactta actgcatcag ttcactgtca
     6421 gtcatcacaa cctctgtcag tcaaaaattt atgccggaca agatagcact ttcgtcagga
     6481 agatggtgtt ggcgttgcaa ttttacgagg aggattcaga aaaaagctga ttagccagag
     6541 ggaagctcac gcccccctct tgtaaatagc tactgtactc gcgccagcgc cacggcttgt
     6601 cctaactgcc agaccgccat tgcgtaatgg gtgctgtggt tgtaacgggt gatggtgtag
     6661 aagttcggca gaccgtacca gtactggtag ccggtgccaa catccagacg cagcaggctg
     6721 gcttgttgat ggttgcccag cggctgctgt ggcgttaaac ctgcggcggc aagctgcgaa
     6781 atgctgtact tagttttgaa gccatttggc aagcctggag cctgaccgtt tgccattacc
     6841 gcgacctgat cgcctttcac ccagccgtgc gctttgaaat agttcgccac gctaccgatc
     6901 gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt
     6961 ttgtaagacg acggcataaa ctgtccgtag cccatcgccc cggcaaagga acctttcaga
     7021 ttgagcggat cgtcctgctc gtcgcgcgcc atcaacagga aggtttccag ctcgccagag
     7081 aaatactccg cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc gaggatgcga
     7141 gttttcccca tcacgcgccc ccagcgggtt tcaacgccga taatcccgac gataatttcc
     7201 ggcggtacgc catacacctg ccacgcgcga ttcaacgcat cttcatactg attccagaaa
     7261 accacaccgt tctgcacgtt gtccggcgta ataaattttt tgcgataacg cagccatgcg
     7321 ccgttcggac ctgatggcgg tttcaccgat gtggttggtg cctggttatc catcagccgc
     7381 agtaccgaat ccagacgttt cgcctgggag agaatttcct gcaactgctg acgatcgaaa
     7441 ccgtgtttgt tcaccatttt gtcgatgaac tgctgggcat tcgggttatt agcgaaatcg
     7501 ccgcccatct gcatcacatt gtgctgcggc tcaagcagga agccgccaga cggcgttccg
     7561 gtggtcgtat cagtctcagt aggttttggc ttgctgctac aggcggcaag caacacaaaa
     7621 aggggaagca atgttacata acgacgcttg aacatgaggg gtccatttaa cagattcaac
     7681 caggggcaag tatggtaaag catcacgccc cgcacaagga agcggtagtc actgcccgat
     7741 acggacttta cataactcaa ctcattcccc tcgctatcct tttattcaaa ctttcaaatt
     7801 aaaatattta tctttcattt tgcgatcaaa ataacacttt taaatctttc aatctgatta
     7861 gattaggttg ccgtttggta ataaaacaat aaatcctgaa ggagagaaca atgatagaaa
     7921 ccattactca tggtgcagag tggtttatcg ggctgttcca aaagggcgga gaggtgttta
     7981 ccgggatggt gaccggcatt cttccgctgt tgattagcct gctggttatc atgaacgcac
     8041 tgattaattt tatcggtcag catcgtattg aacgttttgc tcaacgttgc gccggtaacc
     8101 ctgtttcccg ttacctactg ttaccgtgca ttggcacgtt tgtcttttgc aatccgatga
     8161 ccctaagcct gggtcgcttt atgccggaaa agtacaaacc cagctactac gcggcggcct
     8221 cttatagctg ccactcaatg aatggcctct tcccccatat caaccctggc gaactgtttg
     8281 tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc ccactggcgg
     8341 tgagttatct gctggttggt ctggtcacca atttcttccg cggctgggtg accgatctga
     8401 ccaccgccat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt cacctggcag
     8461 gagcaacatc atgacgcata ttcggatcga aaaaggaacg ggtggctggg gcggcccgct
     8521 tgagctgaaa gccacgccgg gaaaaaaaat cgtctatatc accgccggta cccggcctgc
     8581 gattgttgac aaactggcac agcttactgg ctggcaggct attgacggat ttaaagaagg
     8641 tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg
     8701 cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg gcaagtccgg
     8761 tccgctggcg cagtacattg tggaagatat ttatgtctct ggcgtaaaag aagaaaacat
     8821 cactgtagtg ggtgatgcga caccacaacc ctcttccgtg ggccgtgact atgacaccag
     8881 taagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg gcatggggtc
     8941 cgccgttgcg gtgctgtttc aatctggtcg tgacaccatc gacactgtat taaaaaccat
     9001 tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt ctggccttgg
     9061 tgactggatt gcccacggtc ttgctccgct ggcgagccat ccactgggtc tggtcatgct
     9121 ggcgctcatc tgctccttcc cactgctttc acctttcctc ggcccaggcg cagttatcgc
     9181 acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc cgccgcatct
     9241 ggctttacct gcactgtttg ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg
     9301 tttgtcgctg gcggaagctc gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt
     9361 gagccgcttt ttaaccggcg cgccgactgt actgatcgcc tggtttgtct ccggttttat
     9421 ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc cgtatcggcg
     9481 cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag
     9541 cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt gcactccatc
     9601 ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg
     9661 cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag
     9721 cggaatttcc gggcactgtc catgtggcag gccctgtacc cgacgatatc gcgccgggat
     9781 cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg
     9841 gtggtgggca aacattaggc gcgttcctgt gccacggtct ggctgccgag gggtatcgcg
     9901 tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg
     9961 aatatggtga aagtatggcg tacggttttg gtgctgacgc cactagcgag caaagcgttc
    10021 tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg gtctacagcg
    10081 ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt gaccgttcgc
    10141 tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc
    10201 gcgacgggat tcagggtcgc attattcaga tcaactcgaa atccggcaaa gtgggcagca
    10261 aacacaactc tggctacagc gcagcgaaat ttggtggcgt cgggctgact caatcactgg
    10321 cgctggatct ggcggagtac ggcattacgg tgcattcact gatgctcggt aacctgctga
    10381 aatcgccgat gttccagtca ctgttgccac aatacgcgac caagctgggt atcaaaccgg
    10441 atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc gattatcagg
    10501 atgtgctgaa tatgctgctg ttctatgcca gtcctaaggc gtcgtactgc accggacagt
    10561 cgatcaatgt caccggcggt caggtgatgt tctgatcaac agcggagatc cgttaaggat
    10621 ctccgtgaga ctaatagaat gcctgatgcg ctacgctcat caggcctaca ggacttccgc
    10681 cactacatta aggaaaagtt atggtatccg cactcatcac cgtcgccgtt atcgcctggt
    10741 gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac
    10801 tatgccagca aggtcgggtt ggcgtgggcc gttccagcgg gcgctttaaa ccgcgggtcg
    10861 tggtcgccat cgcgctggac gatcagcagc gtatcgtcga taccttgttt atgaaaggac
    10921 tgaccgtctt cgcacgaccg caaaaaattc ccgcaattac cggcatgcat gcgggtgatt
    10981 tacagcccga tgtgatcttt ccccatgatc cactatcaca gaatgctcta tcattggcgc
    11041 ttaaactgaa acgtggataa tttcgttgtg aatgttactt gcttgcgaag ttatcatttt
    11101 gaaacctaaa tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc gccattctgg
    11161 agtatctgca aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca
    11221 caggcacaac cattcgcaaa gatctggtca ttctggaaca tgccggaacc gtcattcgta
    11281 cttatggcgg agtggtgttg aataaagagg aatccgatcc gcctatcgat cataaaacac
    11341 tcatcaacac ccacaagaaa gagctgattg cagaagctgc cgttagtttt atccatgatg
    11401 gcgattcgat cattcttgat gctggcagta ccgttttgca gatggttccc ctgctctcgc
    11461 gctttaataa catcacggtg atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac
    11521 tggataacga acaaactatc ctgatgccag gcggaacgtt tcgtaaaaaa tcggcctcat
    11581 ttcacgggca gctggcagag aatgccttcg agcatttcac cttcgataaa ttgtttatgg
    11641 gcaccgacgg catcgatctc aatgcgggcg taaccacgtt caacgaggtt tataccgtca
    11701 gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat ggcggactca tcaaagtttg
    11761 gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt cgataagctg attaccgacg
    11821 caggtatcga tccggcgttt cgtcaggcgc tggaagagaa agggatcgat gtgatcataa
    11881 ccggagagag caatgagtga agcactactg aacgcgggac gtcagacgtt aatgctggag
    11941 ttgcaggaag caagccgatt accggaacgt ctgggcgatg actttgttcg cgccgccaat
    12001 atcatcctgc actgcgaagg caaagtggtg gtttcgggaa ttggcaaatc gggccacatt
    12061 ggtaagaaaa tcgccgcaac acttgccagt accggcacac cggctttttt tgtccatccg
    12121 gcagaggcac tgcacggcga tctggggatg attgaaagcc gcgatgtgat gctgtttatc
    12181 tcttactccg gtggcgcgaa ggaactggat ctgattattc cgcgtctgga agataaatct
    12241 aacgcgctgc tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc ggcaaaagcg
    12301 gtgcttgata tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc gacctccagc
    12361 accgtcaata ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca ggcgcgcgga
    12421 tttaatgaag aagattttgc ccgctcccac ccagccgggg cactgggcgc tcgcttgctg
    12481 aataaagtgc atcatctgat gcgccgtgac gatgccatcc cacaggtggc gttaaccgcc
    12541 agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc tggggctggt ggcggtatgt
    12601 gacgctcaac aacaggtaca aggcgtcttt accgacggcg atttacgtcg ctggctggtt
    12661 ggcggcggcg cactcaccac gccagttaat gaagcgatga cggtcggcgg caccacgttg
    12721 caatcgcaaa gtcgcgccat cgacgccaaa gagatcctga tgaagcgcaa aatcactgcc
    12781 gcaccggtgg tagatgaaaa cggcaaactc accggcgcaa ttaacctgca ggatttctat
    12841 caggccggga ttatttaatc cttcattccc agacgtttcg ccagccgatg caggttggcg
    12901 acgtcggttt ccagcatccg cgcgcaggca gcccagttgt gatgattttg tgccagcgcc
    12961 tgacgaatag tttcacgctg gaacgcttct gtcgcttcac gcaggttttg cttaacaacg
    13021 ggcaccgccg ccgcttctgg cggcggcaac gtcacctcag gaaaagcaaa atgttgcgcc
    13081 tcaagaatca cttcatcgcc gttgcgggtg gctctcgaca gcactaccgc ccgatgaata
    13141 gcatgttcca gttcgcgcac gttccccgga aaacgatagt gttgcagtaa atttcgcgct
    13201 ccggcactta ataccacgcg ggagagcccc agccgcaaac gacactgctc gcagaaatac
    13261 cccgccagca gaatgacatc atcgccccgc tcacgcagcg gcggcaccga aagtggaaac
    13321 acgctcaggc gatgaaacaa gtcagcgcga aatcgccctg ccagcacctc ttcgcgtaag
    13381 tcgcggttag tcgccgccag cacgcgcaca tcgacccgca aactgcggtc atcgccaacg
    13441 cgctgaatat cgccatactg caacaccctc agcagcttgg cctgcaatgc caacgacaac
    13501 tcgccgatct catccagaaa cagagtgccg ttatccgcca tttcaaactt cccgctgcgg
    13561 ttactgatag cgccagtaaa cgctcctttc acatgcccga acagttcact ttccgccaca
    13621 ctttccggca gtgcggcaca gttgagatag accagcggat tcaccgcccg tggcgaggct
    13681 tcatgaatcg ctttcgccac cagctcctta ccggttcccg tctcaccgct gatcaggacg
    13741 ttgagatcgg acgccgccac aatctcaatc tcttttttca gttgcgtcat gccaggggac
    13801 aagccaatca tctgcgtctg tttcaccgct tcaaacggcg tggcatcgcc tggcatcata
    13861 ttctggcttt ccagttgttc aatcagcaac gcattgctta acgctcccgc cgccagcgca
    13921 gcaatcagcc gtagctcttc gtcgctgaaa acatcgaact gatcgggctg catcccgtcg
    13981 agcgtcagtg cgccgatcag gttttgcccg gcaaacaatg gcagaccaac gcaggcgtga
    14041 accttcagac tctcctgccc aggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct
    14101 gcgggaaagc gcaccacatc cccggcgcgg gcaatcgctt ccagccgtgg atgcccttcc
    14161 agcgcaaagc gtctaccgag tacatccttt gccagaccgt cgatggcaag cggaataaac
    14221 tgccgcgaat cgtaacgtag caacgcagac gcatcgcact ccagcacctg acgtagcgtg
    14281 gtgatcaggc gctgaaaacg atcctgatga ccaatcccac gctgcaattc gatggcgata
    14341 ttcgccagca catcaacgga aaaactcatc tttgcctcac tgtcaatttg actatagata
    14401 ttgtcatatc gaccatttga ttgatagtca ttttgactac tcattaatga gcatactttt
    14461 atttatatag taaaaacaat cagataaaaa actggcacgc aatctgcaat tagcaagaca
    14521 tctttttaga acacgctgaa taaattgagg ttgctatgtc tattgtggtg aaaaataaca
    14581 ttcattgggt tggtcaacgt gactgggaag tgcgtgattt tcacggcacg gaatataaaa
    14641 cgctgcgcgg cagcagttac aacagctacc tcatccgcga agaaaaaaac gtgctgatcg
    14701 acaccgtcga ccataaattc agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc
    14761 tggcggatat cgattacatc gtgattaacc atgcagaaga ggaccacgct ggggcgctga
    14821 ccgaactgat ggcgcaaatt cccgatacgc cgatctactg tactgccaac gctatcgact
    14881 cgataaatgg tcatcaccat catccggagt ggaattttaa tgtggtgaaa actggcgaca
    14941 cgctggatat cggcaacggc aaacagctca tttttgtcga aacaccaatg ctgcactggc
    15001 cggacagcat gatgacttac ctgacaggcg acgcggtgct gttcagtaac gatgctttcg
    15061 gtcagcacta ctgcgacgag catctgttca acgatgaagt ggatcagacg gagcttttcg
    15121 agcagtgcca gcgttactac gccaatatcc tgacgccgtt cagccgcctg gtaacgccga
    15181 aaattaccga gatcctgggc tttaacttac cagtcgatat gatagcgacc tcccacggcg
    15241 tggtctggcg cgataacccg acgcaaattg tcgagctgta cctgaagtgg gccgctgatt
    15301 atcaggaaga cagaatcacc attttctacg acaccatgtc gaataacacc cgcatgatgg
    15361 ctgacgctat cgcccaggga attgcggaaa ccgacccacg cgtggcggtg aaaattttca
    15421 acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc
    15481 tggtcggcac ttcgacgatg aataacgtga tgatgccgaa aatcgccggg ctggtggagg
    15541 agatgactgg tttacgcttc cgtaacaaac gcgccagtgc tttcggctct cacggctgga
    15601 gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc
    15661 ttagcctgaa agccaaatgg cgaccagacc aggacgctct ggagttatgt cgtgaacacg
    15721 gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg
    15781 tggttaaaga agaaacctct gccaccacga cggctgacct cggcccacgg atgcagtgca
    15841 gcgtctgcca gtggatttac gatccggcaa aaggcgagcc aatgcaggac gttgcgccag
    15901 gaacgccgtg gagtgaagtc ccggataact tcctctgccc ggaatgctcc ctcggcaaag
    15961 acgtctttga agaactggca tcggaggcaa aatgagtaac ggcattgtga tcatcggttc
    16021 gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca ctattccatt
    16081 aaccctgatt gccgccgaca gcatggatga gtacaacaaa cctgacctca gccatgttat
    16141 cagtcagggg caacgtgccg atgaccttac ccgccagacg gcgggtgaat ttgccgagca
    16201 gtttaatctg cacctgtttc cacaaacctg ggtgacggat atcgatgccg aagcccgtgt
    16261 ggtgaaaagc cagaataatc agtggcaata cgacaagcta gtactggcaa ccggtgccag
    16321 tgcctttgtc ccgcctgtgc ctgggcgtga gttaatgctg acgttaaata gtcagcaaga
    16381 gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg ttggcggtgg
    16441 tttgattggt agcgaactgg cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat
    16501 cgacaacgct gccagtattc tggcgtcatt aatgccaccg gaagtaagca gccgcttgca
    16561 gcatcggttg acggagatgg gcgttcatct gttgttgaaa tctcagttgc aggggctgga
    16621 aaaaacagat tctggcattc tggcaacgct ggaccgccag cgcagtatcg aagtggatgc
    16681 ggtaattgcc gccaccggac tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac
    16741 gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg atatttacgc
    16801 gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg ttcctccagc cgattcaact
    16861 tagcgcgatg gtgctggcaa aaaatcttct cggcaataac acgccactga aactaccggc
    16921 gatgctggtg aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg aaacccagcg
    16981 tcaggattta cgctggcaaa ttaataccga acgccaggga atggtggcgc gcggcgttga
    17041 cgatgctgac cagcttcgcg cctttgtggt cagtgaggat cggatgaaag aggcatttgg
    17101 attgttgaaa acattgccga tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc
    17161 tggcgcgtct tatccaacct acggggagcg catgtgttgg tcacatactg cgttcatgtc
    17221 gcatccacct actgtgccca aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac
    17281 ggggacgcat gtgtaggccg gataaggcgt ttacgccgca tccggcaatg gtgtccaaat
    17341 gcaacacgtt ttatccgttc tggacttcac ccgctaacca acgcgccgca gcaataaccc
    17401 cctgccccag agacaaaccg ccatcacccg ccggtaaact ctgtggaaag agcaatgtga
    17461 aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata accccgccgc
    17521 taaataccag cgtagtgata ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac
    17581 cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aaccggtgcc tgccagttca
    17641 gccactgctg ccagaaagtg gcgagatcca gttgattgtc caccagcggc attgtcaccg
    17701 gatgcgtcac tccgtggcac gaagctgcga gtgcctccag agcacaagcc gcttcacctt
    17761 cataacttaa cgtggctggc gcacagccca gtgccgccgc cacggcatcg aacaaccgcc
    17821 cacacgatga cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agcacgctcc
    17881 agttttgctg ttgcacactt gctgtttcag aataattctg ccactccggc acaaagcgca
    17941 ggcactgcgc cagcaggttt cgccacggct gcttcgccgc caaatcgcca cccggaagcg
    18001 ccactgcagg caagccgccc aggtgctcac attcgcgata gttcacccgc agacactcgc
    18061 cgccccacaa agcgccgttc tcccccatac cgataccatc gagcgtcaaa gcaatgacat
    18121 caccgccatc cagcggccac tgatgctctg ccagacacgc cgctgcgtgg gcatgatgat
    18181 gcagcaccgt ttgcgtcggc agattcattt cgcgcgccca ctggctggag acatagcccg
    18241 gatgtgcgtc atgcacaacg tattgcgggg tgaaatcgta gatgttttgc atcaggcgta
    18301 acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctgactca
    18361 acactgcttg ttcaccgcgc accaggcaga aggtgttttt cagatctgcg ccgagacaca
    18421 gcacaggcgg cacattttta aagcccggag gcaaagccag tgcgtccggt acataccccc
    18481 gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga atcatccatc cgctgcacga
    18541 tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt
    18601 cgttgctgat agccggtggt ttaccgctca ggttgccgga ggtcatcacc agcgggcatt
    18661 gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacatcacg ccgacttcgt
    18721 taaggccagg ggcgatatca tcacaaagct caggaacgta ttttttatcc accagcacaa
    18781 tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc tggtaaacct tctgccactg
    18841 gcaagatgac cgccagcggt ttcgccgggc gatgtttgcg cgcccgaagt gtcgccaccg
    18901 cggtactgtt acgtgcatcg caggcaagat gaaatccgcc aatccctttg atggcgacaa
    18961 tgttgcccat ttttaactgt gcgatagccg cctgtaatgc cgcctcttgt tccgcatgtt
    19021 caccatgact tacccattca agatgtgggc cacactccgg gcaggccacc ggctgggcgt
    19081 ggaagcgacg atcgagcggg tcgcggtact ctttatcaca ggctggacac agcggaaact
    19141 ccgccattac ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg aaacgcgggc
    19201 cgcagtgggt acagttgata aacggataac gataacgccg ttcgcctggg gtattcattt
    19261 cggcaaggca agcagggcaa gtagcggcat ccgggacaat ttgcgtattc atggcacctc
    19321 ccgcgctctg gcggatagtg aactcggtgg gcagttgtga ccagataaac ggctcacgct
    19381 cgacgctatc aatacgcgcc agtggcggac agtgctgatg caattgaaca agaaacgttt
    19441 ccgggtcttc cagcagccgg acttctacgc catcgccgtc attacagaca tcgccgtgaa
    19501 gatttaattg ctgtgccagc tgccagacaa acggacgaaa accgacgccc tgcactttgc
    19561 cacgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat
    19621 catgaattgc gtaacccgtc cagccggaca cgacagcgtc gcatccggca gtcacaggtc
    19681 ggcgataccg cccgcttcgt attctacgaa tatttccggg aattcctttg atgccagaac
    19741 agttctgtaa gatttttaga acatcagcgc cgtacggcgg cgtttttctg cgctcagttg
    19801 ttcaagttta ttacgatcga cacaaattag cgcatgggtc gggcaagccg ccatacacgc
    19861 cgggccgtct tcacgatggt tgcacaggtc gcatttattg gcttcagctt tgtcagcccg
    19921 tacattcagc cccgcgccgc tgttgcggat caccggacgt accaccactt ccatcgcgcc
    19981 atacgggcaa gccaccacgc aggttttgca accaatgcaa cgttcctgca tcacatgaac
    20041 aaacccttta tcacggctga tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc
    20101 acactgacgg cagactgtcg ccgtggaaat gttcacacct ttaatgacat ggattcgcgg
    20161 taaaaaggtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag aaaccacgca
    20221 cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt
    20281 catcaaattc tccagcaatg acagttaatg cgccgataca ttcacaaatc atgccagttt
    20341 ttaatttgct gttatttaag gaaattaatt tctgtaatgc aggaaaaaag atgtcatcga
    20401 cactagtgac gatgacatgt gatgacaatg tttatcgcga aagggcaatg agtgagtcgc
    20461 ggcggatcag ttttccgctg aaggttttcg gcggtgagaa atccccgcca tcgagcataa
    20521 aaatcagccg tccaataatt tcctgaatca tctctgttac cggaattttt acgctggaga
    20581 gcgccggaac ggtgtagggg gcaattgcga tatcatcgaa tccgataact gacacctgct
    20641 ctggcaccgc tacgccgcgc tcgtgcaacg ctttcatcgc acctatcgcc atatcgtcgt
    20701 tactggcaac taacgcgcta aatttagccc cacgttcgag caacgtttct accccttcgg
    20761 ccccgctggc gggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat
    20821 gctgcgccag cgcgtcttta tacccagcaa gacgttcaat gctggtgggg gaatccatcg
    20881 agccggtaag gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcattaa
    20941 agctggtctg tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat
    21001 taagcaccat tatcggctga ctgtgcgcgt caatgatgtc atcaatctca tccacgctta
    21061 aaaaacgcgg gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct
    21121 ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgccgc cctttctctt
    21181 ccgccattcg cgcggcatga aagagtaatt cactaaaata aatgccgtgg taaagcgtgt
    21241 tggtcactac cagccccagc gtctgagtac tcttcgccga cagattgcgc gccagcaagt
    21301 ttgggcggta accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga
    21361 cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg
    21421 ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact
    21481 gcaccgtttg ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc
    21541 acaaatataa aaaatgaaca attaactctc ttgcttattt agtgacaact attcatgatt
    21601 ttgtgaaacc ggtttcctaa ttccgtttca gcatcggcat ttttccgtta cgtcgactga
    21661 taacaactac atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg
    21721 cgctggcacg ctcggtgata gcggcactgg gcggcgttga taacatctcg gcagtcacgc
    21781 actgtatgac gcgtttgcgc tttgttatca aagatgacca gcttatcgac agcccgacgt
    21841 taaaaaccat ccccggcgtg ctcggcgtgg tacgtagtga caaccagtgt caggtgatta
    21901 tcggcaatac cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc
    21961 agcccgcaca gcccgtgggt aaacccaaac tcacgctgcg tcgcattggt gcggggatcc
    22021 ttgatgcgct gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg
    22081 tcaaactgct ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct
    22141 taaccattct gaatgtgatt ggtgacggtg ctttcttctt cctgccgcta atggtcgcgg
    22201 catctgccgc catcaaattt aaaaccaata tgtcgctggc gattgccatc gcaggtgtgc
    22261 tggtacatcc gagctttatt gaactgatgg cgaaagcggc ccagggtgaa catgtcgaat
    22321 ttgccctgat tccggtcacc gcggtgaaat atacctacac ggtgatcccg gcgctggtga
    22381 tgacctggtg cctgtcatat atcgaacgct gggtggatcg cattacaccg gctgtgacca
    22441 aaaacttcct caagccaatg ctgattgtgt tgattgccgc accgctggca atcctgctga
    22501 ttggcccgat tggtatctgg atcggtagcg ccatttcggc gctggtttac accattcatg
    22561 gttatctggg ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga
    22621 ccgggatgca ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag
    22681 aagggatggt catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg
    22741 cggtggcgtg gaaaacgaaa aacccggaac tgcgccagac ggcgctggct gcggcggcat
    22801 cagccattat ggcggggatt tccgaaccgg cgttatacgg cgtggcgatc cgcctgaaac
    22861 gtccgcttat cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg
    22921 ggcttgccag ccactcaatg gcagcgccgg ggttatttac cagcgtgcaa ttcttcgatc
    22981 cggcgaatcc aatgagcatc gtctgggtgt tcgcggtcat ggcgctggcg gtggtgctgt
    23041 cgtttatcct cacactattg ctcggctttg aggatattcc tgttgaggaa gcggctgccg
    23101 aggcgcgaaa gcatcagagc gcacaaccga ccgtcgccaa agaagtaagt cttaattgag
    23161 gatgaaaatg tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca
    23221 gtctgaaggt gcgttccgtg aaggtggcaa aggactgacc acggtcgata tgatcccgca
    23281 cggcgagcat cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga
    23341 cgagttttat cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc
    23401 cctgatggca gagatgggat tcaaggtttt ccgtacctca attgcctgga gccgcctctt
    23461 tccgcagggc gatgaaatca cgcccaatca gcagggcatt gctttttacc gttctgtctt
    23521 tgaagagtgt aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc
    23581 gatgcatctg gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag
    23641 ccgctacgcc agaacctgct ttgaagcatt tgatggtctg gtgaaatact ggctaacctt
    23701 caatgaaatc aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga
    23761 aggtgaaaat caggatcagg tgaaatatca ggccgcgcat caccagctgg ttgccagtgc
    23821 gctagccacc aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc
    23881 gggcggtaac ttctaccctt acagctgcaa accggaagat gtctgggcgg cgctggagaa
    23941 agatcgggaa aacctgtttt ttatcgatgt gcaggcgcgc ggcgcgtatc cggcttactc
    24001 tgcccgcgta tttcgcgaaa aaggggtaac catcaacaaa gcaccgggcg atgatgaaat
    24061 cctgaaaaac accgtcgatt ttgtctcttt cagctattac gcctcgcgct gcgcctcggc
    24121 ggagatgaac gccaacaaca gcagtgcggc gaacgtggtg aaatcgctgc gtaatccgta
    24181 tctacaggtg agcgactggg gctggggaat tgatccactc ggtctgcgta tcaccatgaa
    24241 tatgatgtac gatcgttatc agaagccgct gtttctggtg gaaaacggcc tcggcgcaaa
    24301 agatgaactt gctgccaatg gcgagattaa cgatgactat cgcatcagct atttacgcga
    24361 acatatccgc gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac
    24421 ctggggctgt attgatttag tttccgcctc tacgggtgaa atgagcaaac gctacggttt
    24481 tgtctacgta gaccgtgacg acgcaggcaa cggcacgctg acgcgcacgc gtaagaaatc
    24541 gttctggtgg tataaaaaag taattgccag taatggggaa gatttagagt aagtaaccgt
    24601 gccggatgcg gcgtgaacgc cttatccggc ctacatgtcg cacgaaccat gaaggcccga
    24661 taagcgcagc gcatcgggca atttagcatt tggcatcaga gctggttcac cccatcaaga
    24721 acatccctgt cctgattcct taatgaaaaa ctactctact tccaccgcta actgcgcaaa
    24781 tccaccattt ccttcccagc cttccagtcg ttgataaacg gtttcaaccg catctttaat
    24841 cggctgggtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    24901 ttcgccaata tcttctttca actggtcgat aaggtagttc aacggcatgt tatgggtagt
    24961 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatctcgc cggggtttag
    25021 ccccatatca gtggcgtcga caatcagcag tcgcgtaggg cgcagttcac ggatggcgac
    25081 gatgtcgttt tccggtgcac taccgccgtc aatcaccacc cagttacctt tcggcgcggc
    25141 ggcgcacttt tccgccagca gcgggcctgc gccatcatcg cccatcatgc tattgccaac
    25201 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    25261 gaatatcatg cagcatactt agcagcattg tactccattc ctgctgctgc gaagtttgcg
    25321 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    25381 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcgctgccc gcctccagag
    25441 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    25501 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    25561 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    25621 tcatcggata cgcgcctctt ctacaacatg attcagatgg ctgacaatct cgttcagacg
    25681 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    25741 caggaagtca tcggcaatct gacgaccgta acgataacct gccaggcggc gtgcttcgcg
    25801 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg ccggttgttc
    25861 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    25921 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca gggatgtaga catctaccgg
    25981 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaagatcc cgccactgtt
    26041 accgcaggca ccgtaggaga tacagatttt cgggtccggc gcggactgcc acgcacgcag
    26101 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    26161 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    26221 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcggtaaa cgtaggcaga
    26281 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    26341 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    26401 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    26461 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcacc attgtgctta
    26521 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcga
    26581 cagctgcaca gcacaaaacg ggactgttgc aggaaatctt ctttcttcca caccgccagt
    26641 tcgtactctt gcgacagttt gatcgctgcc gtcgggcaga cttcttcgca gcgtccacag
    26701 aagatgcagc gcccgagatt aaactcccac gcaagctctc ctgtggcgag gtcagcttca
    26761 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cgcagccgat gcactgctgc
    26821 gggttctgct ctggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    26881 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    26941 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    27001 actttgctct tcttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    27061 gggtccaggc taccgataat cagcggcgca tcggaaacgg tattgccacg cagcatgtag
    27121 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    27181 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    27241 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccacc cggcaagtta
    27301 tccagaccgt agtcgatcat gttcagcgcg gtatagactt cgttgatacg cactttcaga
    27361 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataacca
    27421 acaaacgggt gatcggcgcg ggtatcgcga gcgtgtccgc tggcgcggac catcgggcca
    27481 acgttactga aatcgcgggc gatttccggg tccagacgac caatgccgac agtgcgctgt
    27541 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatttgt
    27601 tgcgccagct ggcgggtctg gatcatgtca tctttcagca gatcgcgacg aatcccgccg
    27661 atcaggttca ggccgtaggt tttacgcgca ccggtaagga tctctgccat tttcatggag
    27721 gtttcacgca cgcggaagaa ctgcataaag ccggagtcga agccagtaaa gtgacaggcc
    27781 aggccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    27841 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg
    27901 gtgctgtgag caaagccgca gatgccgcac acacggtcag agaggaaggt cacttcgttg
    27961 taccccatac gggtttccgc cagtttttcc atgccgcgat ggacgtagaa caggcggtag
    28021 tcggcgtcga taatattttc gccatcgacg aacagacgga agtgacccgg ttcgtcggaa
    28081 gtgacgtgca acggaccaat cggcacgacg ttgtttttct tgtcacccag ttcattgatg
    28141 aactcgtagg tttcagcatc ggtggtcggc gccggacgct gacgataatc catgctgtct
    28201 ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg acgttcatcc
    28261 ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc ccacacagcc
    28321 gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcgtcaac ttcgacgcga
    28381 accgttaccc aacacttagt gcccttctcc atcgacagca cgtaataaac ggcgtaatga
    28441 ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca gccagccacc ctgtttgtag
    28501 taaagaaact ccaccacttc cggcaggtag ttcaccttta cggtgacagt cagctgatct
    28561 ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg cctcattcag cgcggcgaga
    28621 taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac gccgccagca
    28681 aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc ggagtaatat
    28741 caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg ccgaccacca
    28801 gtttgacgat ggcaatcacc agcgccagca gcagtccacc agcggtgaag gtttccattt
    28861 gtccccacgg aataaacacc ccgacgaaca tctgcaacac caccagctgt ttcaggctga
    28921 taccccattt catgacgcca aagccgctac cgctgtattc ggagagcgga ccttcctgca
    28981 actcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata aaagtggcga
    29041 acgcacaggc acaaagcgcc agcaccagcg ggatgctctg gctcagcggc cagtgataaa
    29101 cggtgtcggt gatgttgctg atgttggtgg aaccggcaac ctgtgcggca acccacagac
    29161 caagcagcag catcggttcg accagcacgc caagcatcgc ttcacggctc gcgccgatag
    29221 cagtaaacgg gctaccggta tccagaccgg aaatagcaaa gaagaaacgc gcgatggcaa
    29281 agagatacag taaggtgatc aaatcgccca gttgcggcag cggagaaccg acggtcacca
    29341 ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc gtcaggcgga
    29401 acacccagcc ggaggcatcc gggccaacgc tctggcggcc cagcagtttg aaaatgtcac
    29461 gatactcctg caacacaccc ggcccacgac ggttatgcag acgggcgcgc gccacgcggg
    29521 taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt aacggatata
    29581 aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg ccagttcaac
    29641 cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg gcaccagcga
    29701 caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt tcaccggcat
    29761 ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc acacccaggc
    29821 cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg
    29881 cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg tggtgttagc
    29941 aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg gcgcagcaac
    30001 accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca ggagcggcgc
    30061 acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga cgccatagac
    30121 tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag cgagcagcgg
    30181 ccccagcaga cgggcaacaa acgcgccgct attgctcagt ttgaagaagg attgatagat
    30241 aacccattcc ccggcaaacc cgttcagcgg cggcagcgca gccattgcca ttaacccgac
    30301 taacatggcg atggagataa ccggcatttt cttgccaata ccaccgagtt tttcgatatc
    30361 gcgatgaccg gtacggaacc agacgctccc cgctccgagg aacagtacgc ttttgaacag
    30421 gctatggtta agcagatggt acagaccacc gaccaggcca agagcaatca gtgccggttg
    30481 ttcgagcgcg atacccgtta cgccagcgcc cagccccagc agaataatgc cgatattttc
    30541 cagggtgtgg taagccagca ggcgctggat gttgtgttcc atcaacgcat acagaccacc
    30601 gacgaacgcg gtgatcatgc cgagcaccag cagcgcgatc ccccaccaca gcggtgcatt
    30661 accgccaagc agtgacaggg ttaacatgcc cagcaggcca attttcatga ctaccgtaga
    30721 aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag
    30781 cggaataatc ccggccagca ggccaaagcc aatcacaccg agcagccaga tatcggaacc
    30841 gagcggcagc tgttgcatac gcatatccag caggcgcaga tccagcgtgc cataacgctg
    30901 ccacagcagc cagcaagcaa tcgccagcag cagagtgcca agacgcccca gcgcaaacca
    30961 cagtttgccc tctttgctgt tgctggtaag gaacaccgca cacaaggcca tgacttcagc
    31021 cattaccacg aacatgccga ggttgctggc gatgacggcg cagacggcgg cagccatcaa
    31081 catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta
    31141 gaggctgaca aacagaccgc acagaccgag cgtaatcagc caaatcgcgt taagcggaga
    31201 gatttgcaca tcgtagctta ccagcgacag cgcaccgctc acgccaaccg cgccagtcag
    31261 cacagtaaag cccgcggctg ccgtatacag gctaccaacc gcgccgccaa ttccggctat
    31321 ccagccgctt agcgcctttt gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa
    31381 ccacgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    31441 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    31501 ctaacgcggg cgatatcggt gttatccacc agatgcaggg ctttggtcgg gcacatccgc
    31561 acgcaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    31621 atacccggca cccagtcaag cagcgtactg atacgcgccg gagcaggcgg tgccggtggc
    31681 gctttcgggg tattggcatt tgcaggaata tccagcggac ggctgccgga aaattcaatt
    31741 gcgccaaacg ggcaggcgat gccgcacagc ttgcagctta cgcacaggct ttcattcaac
    31801 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    31861 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattcagcat cactctcagg
    31921 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg
    31981 tggcagccga tacagagcgt ggagtcagca attacaaaac gattcaccag gcattcctca
    32041 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    32101 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    32161 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    32221 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    32281 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    32341 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    32401 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    32461 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    32521 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    32581 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttacccgttt aacagagatg
    32641 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    32701 ataaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    32761 cgcagggata atcaacactg gcacaattat tgcttgtagc tggcaatagt taatgggagg
    32821 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    32881 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    32941 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    33001 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    33061 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    33121 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    33181 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    33241 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgaaga tgaaaatcac
    33301 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    33361 tcatggcgaa gcgggcactc acgcgccggg catgagccag cgtcggatgc tggaagtcga
    33421 aattgacgtg ctggacaaaa ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    33481 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    33541 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcagttattg aaggcgatca
    33601 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc acaccagcga ttcaggtgaa
    33661 cactggtaaa ggctgccacc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    33721 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    33781 cttcgatctc ggtgaaaaac acaaagtagc ggtcctttcc gttaccgaag gtgaagacaa
    33841 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    33901 ccttttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgcac gcgaagtcaa
    33961 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    34021 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    34081 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    34141 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    34201 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    34261 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    34321 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    34381 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    34441 gtcataccca cgctatcttt aaattcggcc tcgaccagtt actgccggaa aacgttgagt
    34501 ttatccacgg tccgggttgt ccggtgtgtg tactgccgat gggtagaatc gacacctgcg
    34561 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    34621 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    34681 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgtaaa gtcgtattct
    34741 tcggcttagg ttttgaaacc accatgccga ccaccgccat caccctgcaa caggcgaaag
    34801 cccgcgatgt gcagaatttt tacttcttct gccagcatat tacgcttatc ccgacgctgc
    34861 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    34921 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    34981 tggtcgctgg tttcgaaccc cttgatctac tacaaggcgt ggtcatgctg gtggagcaga
    35041 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg agtggtaccg gatgccggta
    35101 acctgctggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    35161 gcggcttagg cgtgattgaa tcttctggcg tacacctgac gccggattat caacgattcg
    35221 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcacgtt
    35281 gtggtgaagt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    35341 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    35401 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    35461 cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    35521 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcagaagg
    35581 cgatcgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    35641 taatatcggc aagctggcga tttgcggcac tgcgaatgac gttgcggtca gtggcgctat
    35701 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    35761 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    35821 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    35881 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    35941 actggttagc ggcacgctcg gcgaccacgg gacgactatc cttaacctgc gtgagcagct
    36001 ggggctggat ggcgaactgg tcagcgactg cgcggtactg acgccgctta ttcagacgct
    36061 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg tgaacgcggt
    36121 agttcatgag ttcgcggcag cctgcggttg tggtattgaa atttcagaag cggcactgcc
    36181 ggttaaacct gccgtgcgcg gcgtttgcga actgctggga ctggacgccc ttaactttgc
    36241 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    36301 gttacattcc catccactgg gaaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    36361 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    36421 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    36481 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    36541 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    36601 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    36661 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca
    36721 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    36781 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    36841 ccgcaggtgg gctataccca aaatttggtc actattgcct gatgccactg gcggcggaag
    36901 ggcatatttt tggtggctgt gaatttattc gttatgacga tcgcccctgg agcgaaaaag
    36961 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    37021 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    37081 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    37141 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc gtcttacgca
    37201 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    37261 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    37321 aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat gaacgcatgt
    37381 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    37441 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    37501 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    37561 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    37621 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    37681 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    37741 gtgaaactgg cacgggtaaa gagctgattg cccgcgcgat ccataatctc agtgggcgta
    37801 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggatta ctggaaagcg
    37861 atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc attggtcgtt
    37921 ttgaactggc ggataaaagc tccctgttcc tcgacgaagt gggcgatatg ccactggagt
    37981 tacagccgaa gttgctgcgt gtattacagg aacaggagtt tgaacgtctc ggcagcaaca
    38041 aaatcattca gacggatgtg cgtctaatcg ccgcgactaa ccgcgatctg aaaaaaatgg
    38101 tcgccgaccg tgagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    38161 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    38221 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ttgcgcacct
    38281 tgagcaatat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag
    38341 tattgctaac acgcggcaac gtgctgcagc tgtcattgcc agatattgtt ttaccggaac
    38401 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    38461 ttgttcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc gctgcgcaac
    38521 gtctggggct gaaacgcacg accttgctgt cacggatgaa gcggcttgga attgataaat
    38581 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    38641 gtttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc atcgatgaga
    38701 tgacgcacgg ttaagatcgg atgacgccac agcattcgcg gcccggccca acgcataatc
    38761 tgtttcatct cttcacgctt tgctggctgg taacagtgca ccggacactg cttacaggct
    38821 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    38881 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    38941 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccatactct
    39001 taaggtgtat ttatattaca acttaatttg aaaggggact atggctctga aggaggaggt
    39061 ttattcaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa
    39121 cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat
    39181 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc
    39241 atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat
    39301 acgcccatga tgcagcagta tctcaagctg aaagcccagc atcccgagat cctgctgttt
    39361 taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg
    39421 ctggatattt cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg
    39481 attccctacc atgcggtgga aaactacctc gccaaactgg tgaatcaggg cgagtccgtt
    39541 gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt
    39601 gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttacagga gcgtcaggac
    39661 aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc
    39721 agctccgggc gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg
    39781 caacgcacta atccggcgga actgctgtat gcagaagatt tcgccgagat gtcgctgatt
    39841 gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc
    39901 cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt cgagaacgcg
    39961 ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg
    40021 accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat cattatggat
    40081 gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc ggaaaatacg
    40141 ctggcttctg tgctcgactg caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg
    40201 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    40261 ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc aggtcggcga cctggaacgt
    40321 attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg tatgcgccac
    40381 gctttccagc aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta
    40441 caggcgctac gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca
    40501 atcatcgaca caccgccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac
    40561 gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg
    40621 gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg
    40681 cacggctact acattcaaat cagccgtggg caaagccatc tggcacccat caactacatg
    40741 cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa agagtacgaa
    40801 gataaagttc tcacctcaaa aggcaaagca ctggcactgg aaaaacagct ttatgaagag
    40861 ctgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg
    40921 gaactcgacg tgctggttaa cctggcggaa cgggcctata ccctgaacta cacctgcccg
    40981 accttcattg ataaaccggg cattcgcatt accgaaggtc gccatccggt agttgaacaa
    41041 gtactgaatg agccatttat cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg
    41101 attattaccg gcccgaacat gggcggtaaa agtacctata tgcgccagac cgcactgatt
    41161 gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagtcgagat tggacctatc
    41221 gatcgcatct ttacccgcgt aggcgcggca gatgacctgg cttccgggcg ctcaaccttt
    41281 atggtggaga tgactgaaac cgccaatatt ttacataacg ccaccgaata cagtctggtg
    41341 ttaatggatg agatcgggcg tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg
    41401 tgcgcagaaa atctggcgaa taagattaag gcattgacgc tgtttgccac ccactatttc
    41461 gagctgaccc agctaccgga gaaaatggaa ggcgtcgcca acgtacatct cgatgcgctg
    41521 gagcacggcg acactatcgc ctttatgcac agcgtgcagg acggcgcggc gagcaaaagc
    41581 tacggtctgg cggttgctgc tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg
    41641 caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ccgctacgca agtggatggt
    41701 acgcaaatgt ctttgctgtc cgtaccagaa gaaacttcgc ctgcggtcga ggcactggaa
    41761 aacctggacc cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag
    41821 agtctggtgt aataacaact cccgatagtc ttttgctatc gggaatatta acgacaactg
    41881 acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg tcaaaccatg
    41941 ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt
    42001 gatattcatg gtgaatatca gttattacaa tcccgcttac atcaactctc ttttttcccc
    42061 gaaaccgact tacttatttc ggtaggcgat aacatcgatc gtgggccgga gagtcttgac
    42121 gtcctgcgcc tgctaaacca gccctggttt atctccgtta aaggcaacca cgaagcaatg
    42181 gcgctggatg cgttcgcgac cggcgatggc aatatgtggc ttgccagcgg tggtgactgg
    42241 tttttcgatt taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat
    42301 caccttccac atattattga aatcactaac gacataataa aatatgtcat cgcacatgca
    42361 gattatccgg ggagtgaata tctctttggt aaagaaatag cggagagcga attactctgg
    42421 cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa cggcgctgat
    42481 tattttatat ttggacatat gatgtttgat aacattcaga cgttcgctaa ccagatttat
    42541 attgataccg gatcgccgaa aagtgggcgg ctgtcatttt ataaaataag gtagtctcat
    42601 gcttcttctg tgaagcatga gtaaccagat gttattgcag gccattattc atttttcgct
    42661 accagcaaag agagatcctg cttcgccagc gcgcgactgg cactctccgg caaaccgtcg
    42721 tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc cacctgacca
    42781 tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat cgcccgtttc
    42841 accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga cgatgcagaa
    42901 ataaaagcct gatcaatcat caggctgcgc agcatggtcg cagcggcttc cccgacacag
    42961 gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt actgttgtcg
    43021 agcaagtagt tcgcaataac gaaatcgttt gtgaccacag tcagtgactc catgtgaatc
    43081 agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca gatagataca acttcccggc
    43141 tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc cagcgcagtt
    43201 tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc tccggacacc
    43261 agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt catatgtgac
    43321 acattcattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc taccatctca
    43381 aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg tcctgctgac
    43441 attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa
    43501 taacacacaa tgttaattta tgtgaatcag ctcaccatat cgttatcttc cagcgcttat
    43561 attcacaata tcaaacaaaa tatcacttaa attaacaagg agagcagatg aaaacgggat
    43621 ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca gcactgtcat
    43681 gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc tgcgctacgt
    43741 tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg
    43801 acgctctgct ggtgctggtg gtcaatgcgg cccaggttaa acaggtgctg tttggtgaaa
    43861 caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc actatcgcta
    43921 gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa atgctggatg
    43981 cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc atggcctccg
    44041 gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc ggaaaagttt
    44101 atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac cagttgttag
    44161 cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt gcggggatcc
    44221 cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg atgttcgaaa
    44281 accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc gatatttttg
    44341 ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg ctgccattgg
    44401 cctcaacagc attgaatatg ttcaccagcg ccagtaacgc aggttacggg aaagaagacg
    44461 atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa tcatgatcaa
    44521 gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt ttctggtgga
    44581 aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat
    44641 cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg gtggttgaag ccacacagca
    44701 atcgctggcg gctctgagct ggttgcaaca gcaaggttgc aaacagatct atttcaaata
    44761 ctgctctact ttcgacagta cggcgaaagg taatatcggc ccggttaccg atgcattaat
    44821 ggatgctctc gacacgccgt ttacggtctt ctctccggcc ctgccggtca acggacgtac
    44881 ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg ggatgcgcca
    44941 tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaag cgcaatccac
    45001 agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa ggtgtggatg ccgttcgtca
    45061 agagctggct cgcttacagc aagagggcta ccgctacgcg gtgcttgatg cgctgaccga
    45121 acaccatctg gaaattcagg gagaagcctt gcgcgatgcc ccactggtaa cgggcggttc
    45181 tggtctggcg attggcctgg cccggcagtg ggcgcaagaa aacggtaacc aggctcgcga
    45241 agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct ctcaaatgac
    45301 caaccgccag gtggcacatt accgtcaaat tgcaccagcc cgtgaagttg atgtggcacg
    45361 ctgcctctca accgaaactc tggccgctta tgcacacgaa ctggcagagt gggttctggg
    45421 ccaggaaagt gtacttgctc cactggtttt tgccaccgcc agcactgacg cattggcagc
    45481 aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaactc tattttctca
    45541 actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag gcggtgagac
    45601 ctccggcgta gtcacacaga gcctgggcat aaaagggttt catattggcc caaccatttc
    45661 ccccggcgtg ccgtgggtaa acgcactgga taagcctgtc tcactcgccc ttaaatctgg
    45721 caacttcggt gatgaagcct ttttttcacg agcccaaaga gagtttttat catgagcgat
    45781 ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag ttcattcttt
    45841 cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc tgacgggaat
    45901 ttactggcga caccgacagg ttcatgcctg ggcaatctcg atccgcagcg gctttccaaa
    45961 gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt gctctttcat
    46021 ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca cagcacatgg
    46081 tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg tccgttcaca
    46141 ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc cttattaccg accgggcgat
    46201 aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt tttactggca
    46261 aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa tatggaagag
    46321 ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg ttatctgacc
    46381 gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag ctaatttatc
    46441 catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa aagccgggtt
    46501 cgatgctgtg gaatttctgt ttccctatga ctactccacc ctgcaaatcc aaaagcaact
    46561 ggagcaaaac catctgacac tggcgctgtt caataccgct cccggagata ttaatgcggg
    46621 ggaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacacgcag acatcgacct
    46681 ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg caggcgtcgt
    46741 gcccgctggc gaagacgctg aacggtaccg ggcagtattt atcgataatc tccgctacgc
    46801 tgccgaccgc tttgcgctac acggcaagcg aatattagtt gaagcactca gccccgacgt
    46861 taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg aagaggttgc
    46921 gcgagataac gtgtttattc aactagatac ttttcatgcg caaaaagtag atggcaacct
    46981 gacgcattta atccgcgact acgctgggaa atatgcgcac gtacaaattg ccggactacc
    47041 tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc gcctgttcga
    47101 tgaggtggga tatcagggct ggatcggttg tgaatataaa cctcgtggcc tcaccgaaga
    47161 agggcttggc tggtttgacg cctggcgcta attcgttaaa tccctaatta caacgtaccc
    47221 atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca gggtttccca
    47281 tgtccacaat tacattgtta tgcattgcgt tagctggcgt aatcatgctg ttgctgctgg
    47341 tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg ttagtcgcac
    47401 ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg atgggcggtg
    47461 tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt atgatcgaac
    47521 actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt gacaaacgaa
    47581 ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc tttgatgtcg
    47641 gctttattat tcttgcgcca atcatttacg gttttgccaa ggttgccaaa atatcgccac
    47701 tcaaatttgg cctgcctgtc gctggaatca tgctaactgt tcatgtggcg gtaccgccgc
    47761 atccaggccc tgtcgccgca gcggggttac tccacgcaga catcggctgg ctaaccatca
    47821 tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg aaaataatca
    47881 atacgcgcca atatgcgatg tcggtggaag tactggaaca gatgcaactg gctccggcca
    47941 gtgaggaagg cgcaacaaaa ttaagcgata aaataaatcc accgggcgtc gcgctggtca
    48001 cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttcc gcaacactga
    48061 tgccgccttc gcatcccctg cttggtacgc tacagctgat cggctcacca atggtagctc
    48121 taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc tggagcttac
    48181 aacacaccag cgacattatg ggctcagccc ttcccactgc ggcggtagtg attttggtta
    48241 ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggc aaagcccttg
    48301 ccaatatgct acaaatgatt gacctgcctc tgttaccagc cgcatttatt atttcactgg
    48361 ctctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc gggttactct
    48421 cagaagcggt gatgggattg aatccgattc agtgcgtatt ggtgacgctg gcagcctgct
    48481 ttggtgggct tggcgcgtca catattaatg actcagggtt ctggattgtg accaaatatc
    48541 tggggttgtc ggtagcagac ggtctgaaaa cctggacagt gttaacgacc attctcggtt
    48601 ttaccggatt cttaattacc tggtgcgtat gggcggtaat ttgaccttaa taaaaaggtc
    48661 cgatgggcat cggacctttt atttaacgct taccttccac cagcagcggc gggatgctcg
    48721 gcaccattgg cgcgtcatca atatctttct gcgtcatgcg gaacgcttcg ggataatgtt
    48781 cgcggctggt acggcgcagc ggttcggtat cgcgccaggt ataaaggcaa tgctggcact
    48841 gatataccgt ccagacatct ttcaccggcg atttcgccat cacttcaatc tgttcatcgg
    48901 cacaacgtgg acaaatcatc ttcttctcct tatttacgtg cagccagcat agcggtcagt
    48961 ttttcagccc aggctttggt ttccggtaaa tccaccaccg gttggctgta gtgaccacgg
    49021 ttgtccgggg cgacaggcgt agtggcgtcg ataatcagct tgtcggtgat ccccgcaggg
    49081 cttgagcctg gatcgagttc cagcacggac atattcggca actgcaccaa atcccctgcc
    49141 gggttcactt tcgaggagag cgcccacatc acctgcggca ggttgaacgg gtcaacgtct
    49201 tcatcgacca taatcaccat cttcacgtag cccagaccat gcggcgtggt cattgcgcgc
    49261 aggcccaccg cgcgggcaaa gccgccgtag cgttttttgg tggagataat cgccagcagg
    49321 ccatgggtgt acatggcgtt taccgcctgc acttcaggga actcggcttt cagctgctga
    49381 tacagcggca cgcaggtggc tggccccatc aggtagtcga tttcggtcca cggcataccg
    49441 aggtacagcg attcgaaaat cggcctggtg cggtaagaga ctttatcgat gcgcaccacg
    49501 gtcatgttac gcccgccgga gtagtgcccg gtaaactcac cgaacggccc ttcgatttcg
    49561 cgtttacggc tttcgatgac cccttcgagg atcacttctg aaccccacgg cacatcaaaa
    49621 ccggtcaacg gggcggtggc gatcgggtac gggctttcac gcagcgcgcc tgccatttcg
    49681 tactcggact gatcatattt cagcggcgtg gcccccatca gcgtgatgat cggatcgtta
    49741 ccgagcgtga tcgcaatcgg cagatcttca ccgcgctctt ctgctttatg cagatgcagg
    49801 gcgatatcgt gcatcggcac cggttgcagg ccgagcttac gcttgccctt cacttccatg
    49861 cggtagatgc cgacgttctg cttgccgaag ttatccgggt cgagcggatc gcgggaaacc
    49921 acgcacgctt tgtcgagata gaaaccgcca tcgccatcgt ttaaacgaaa cagcggcagg
    49981 atatcgaaca ggttgatctc gtcgccatca acggtgttct gcgcctaggc tggattggcg
    50041 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
    50101 ttaaccgggg catttggcgg caggccgagg gaaatcgcgt ggttctgcca ggaaccgatg
    50161 gtgttcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
    50221 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcggcgttc
    50281 acttcttcgc tgattttcag taactggccg tggtcatcaa gctcctgtaa aaagctgcgt
    50341 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
    50401 gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
    50461 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
    50521 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
    50581 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
    50641 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
    50701 taacgatcat gccgtcggta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
    50761 ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc gtttccagtt
    50821 caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctccc
    50881 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
    50941 tcagtttcat tgcttatcct cttaattgtt cgtatacgaa cgttattatt aacatactcc
    51001 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
    51061 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttc
    51121 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
    51181 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tatcgaacag
    51241 gtggcactga tagaagcagc tgtcagcacc aaagcaacgt tagcagaaat gttggcaaga
    51301 atggaaaatc gtggcttagt cagacgtgaa catgaccccg ctgataagcg gcgacgcttt
    51361 gtctggttga ctgccgaagg cgaaaagata cttgctgcgg cgatacccat tggtgacagc
    51421 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagctg
    51481 gtgcgcaaga tgatgaacgc atagggggca aatttccgga tgcgacgcta tcgcgtctta
    51541 tccagactac aaatacaccg actcaccaac cgaaagaaaa aaggccagcc tcgcttgaga
    51601 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
    51661 cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
    51721 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
    51781 accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca gccatttgac
    51841 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
    51901 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
    51961 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
    52021 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
    52081 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
    52141 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
    52201 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat
    52261 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
    52321 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
    52381 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
    52441 ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa
    52501 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
    52561 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
    52621 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcatggt tccgccgatt
    52681 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
    52741 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
    52801 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
    52861 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc agcataaaca
    52921 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
    52981 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
    53041 caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt tgtgcttgct
    53101 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt cggcaacatt
    53161 ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg cgacgcaaca
    53221 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc atcggcctgg
    53281 gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg taaggtctga
    53341 ccgacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
    53401 ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg
    53461 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
    53521 tccatctgta ccggctgctg agctactggc tgtactggct ggatttgcgg ttgttgagta
    53581 gcctgaattt gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtt
    53641 cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg cgcattgcca
    53701 ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc
    53761 gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt
    53821 tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccatac
    53881 tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc aggaaaaatc
    53941 caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc
    54001 ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg
    54061 ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt
    54121 tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac gtgcctgcca
    54181 accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat ttttcaggcg
    54241 gcgacgcgcc tgccactgca agcctttaat ccgttcaacc gagcaaacat gctggacaag
    54301 atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca cccgcgactg
    54361 cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg
    54421 cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac
    54481 gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct gaataccttg
    54541 cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc
    54601 acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc
    54661 agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc cggaccggca
    54721 tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc
    54781 gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt
    54841 tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg caacggctct
    54901 ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc
    54961 ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat cgcggcggct
    55021 accgtaccgg aataaataac gtcatccccc agattcggcc cggcgttaat tccggacaca
    55081 acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca atcggtcggg
    55141 gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc
    55201 agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac ctgaacgtca
    55261 gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca
    55321 ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt
    55381 tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat cccaccaatt
    55441 ccagcttaat tgttgcggat acagcaacat cgctctgcgc gcggcttcaa ctttttcgcg
    55501 caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gccttttcga atgccagcgc
    55561 ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt
    55621 atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgctacaa accagctacc
    55681 acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag
    55741 gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg
    55801 tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc cctgcaagtt
    55861 gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat
    55921 atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa ccagggtaaa
    55981 ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac
    56041 ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcctg gcacgcgagc
    56101 gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc tgacttcacg
    56161 ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt
    56221 tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc
    56281 caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg
    56341 gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct
    56401 tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt agtggctttc
    56461 acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga
    56521 atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt gcccagggta
    56581 taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct gtcggcacct
    56641 ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc cgccgcgcca
    56701 agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc cagcaatcct
    56761 ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcatgt
    56821 acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt
    56881 aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc gcacggcctt
    56941 cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg gtaatagtcg
    57001 cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg aaaaattgcg
    57061 gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg
    57121 gttcggcacg tttcatcgta tcgcgcactg gtgcggctag gatccctccc gtgcggctgg
    57181 tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga cgagcggcgt
    57241 catgcaccaa tacccactgc gcgtcgccag cggctttcaa acctgccagc acggaatcgg
    57301 cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga ggaagttgtg
    57361 caaaacggct atcgccagga cttatggcaa tgacgacacg tttcacccgg ggatgcgcca
    57421 gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag
    57481 gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg caaacatcca
    57541 aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc cccgcagact
    57601 gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc ctggtcatgc
    57661 tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt
    57721 cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc tgtgccgcca
    57781 catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac agcgaatact
    57841 gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa
    57901 aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc
    57961 gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa
    58021 cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga aagtacaaat
    58081 tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac cagggtgaaa
    58141 acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc caccactacc
    58201 gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaagaagaa cagcgtgtta
    58261 gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa accgaccacg
    58321 gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt atttgttagt
    58381 gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg ggcatggaaa
    58441 cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta ataattgctg
    58501 taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt caggcgcttc
    58561 gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt tcttatataa
    58621 gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca cttcgataaa
    58681 gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg gcgagataaa
    58741 tgcggtcagc actaccagtc cggcttcaac catcaaattc gccacttcac cgacgcgacg
    58801 gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc cgtggcgaac
    58861 attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct cctccagcgc
    58921 cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca ctacaccacg
    58981 atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc agacgacgtt
    59041 ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca gtgcggaaag
    59101 tggcgacgaa ccagagcatt caattccagt tcgaatgcac tgaattcaga tggcgcagca
    59161 gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt gctcaggcga
    59221 tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa caccagcggc
    59281 tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc aacttcacgc
    59341 tgggtaaggt tattaatatc aacctgatag cgaatgccat caacacgcgc gcgcgtcttc
    59401 ttaccggcaa ttttgatgtc gtaactctgc cctggagaaa gcggctgttc cgccatccat
    59461 accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc cagcagcaga
    59521 tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc tccggcaaag
    59581 gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc cacaccagaa
    59641 ggcagcactt ttacacgttg cccgacttcc acgcgaccgg atgccagcgt tccggcgtaa
    59701 ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat tggctgagca
    59761 tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg accgctgtac
    59821 cacggcatac tttcactttg cgatgccacg ttgtcgcctt ccagtgcaga gagcggcaca
    59881 aagcggatat ccagattacc cggcagctgc ccggcaaagg tcaaataatc ttcacgaata
    59941 cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc cacgaccaga
    60001 tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc gagcacgcct
    60061 ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt cgccatattg
    60121 cgggtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt ctcggtagag
    60181 aaatagcggt aggccacgtc aatggtgatg ccctgttcgc gctcagcttg caggccgtcc
    60241 accagcagag ccagatccag cttttcgccc tgggtgccgt gacgcttact gtcgttatgc
    60301 agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcagacg accaatcaga
    60361 gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct tttatgttgt
    60421 tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc aagtgcggtg
    60481 ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct ggtcgcggtc
    60541 aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga tgatttccgg
    60601 cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca gcgtacggaa
    60661 acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt tgtcatcaat
    60721 catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat atagcggaac
    60781 aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc agttagagag
    60841 cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt gccacagctc
    60901 cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt aaattcgctc
    60961 tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat caaaaccgta
    61021 tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt tcgcgctgcc
    61081 gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca ccagcagttc
    61141 gcagccgtag gctttagcag tacgatcgcg gaactcatac atctcgcgga atttccagcc
    61201 ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct tgcgcgccag
    61261 atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc
    61321 tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag
    61381 tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt
    61441 cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa
    61501 cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg
    61561 tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa
    61621 atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc
    61681 tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt
    61741 ttgctaatac tcaggcacga catattgcta ctttctttcc gggacgcatg accggaactc
    61801 ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca
    61861 gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat cgtaagagct
    61921 ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagcg ccgcagcaga
    61981 tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac gccgatgcca
    62041 atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta ggtgtcatgc
    62101 tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga
    62161 ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg atgagtgaca
    62221 ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat
    62281 tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg
    62341 cgctggcaat tgcgcgcagt cacggaatag ccgcaacgac caatccgggt ttgaataaaa
    62401 attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa gcgggcattg
    62461 ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt tatcagcaac
    62521 gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga ctggataatc
    62581 aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt gacgttatgc
    62641 ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt tgaaaatggg
    62701 agctgggagt tctaccgcag gggcggggaa ttctaagtga tatccatcat cgcatccagt
    62761 gcgcccggtt tatccccgct gatgcgggga acacacatga atgtcggttc agaccgtgtt
    62821 tttacccggt ttatccccgc tggcgcgggg aacacagtgc tggaccattt caaattcaca
    62881 atagattcgg tttatccccg ctggcg
//
LOCUS       DAPHDX010000006        59092 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPHDX010000006 DAPHDX010000000
VERSION     DAPHDX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.006"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            103..774
                     /locus_tag="RH058_000407"
     CDS             103..774
                     /locus_tag="RH058_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDV3136047.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            784..1980
                     /locus_tag="RH058_000408"
     CDS             784..1980
                     /locus_tag="RH058_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV3136048.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1958..2539
                     /locus_tag="RH058_000409"
     CDS             1958..2539
                     /locus_tag="RH058_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV3136049.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2541..3314
                     /locus_tag="RH058_000410"
     CDS             2541..3314
                     /locus_tag="RH058_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDV3136050.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3499..3774
                     /gene="frmR"
                     /locus_tag="RH058_000411"
     CDS             3499..3774
                     /gene="frmR"
                     /locus_tag="RH058_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV3136051.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3809..4918
                     /gene="frmA"
                     /locus_tag="RH058_000412"
     CDS             3809..4918
                     /gene="frmA"
                     /locus_tag="RH058_000412"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV3136052.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5011..5844
                     /gene="fghA"
                     /locus_tag="RH058_000413"
     CDS             5011..5844
                     /gene="fghA"
                     /locus_tag="RH058_000413"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV3136053.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6070..6609)
                     /locus_tag="RH058_000414"
     CDS             complement(6070..6609)
                     /locus_tag="RH058_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV3136054.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6711..7922)
                     /gene="mhpT"
                     /locus_tag="RH058_000415"
     CDS             complement(6711..7922)
                     /gene="mhpT"
                     /locus_tag="RH058_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV3136055.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8197..9210)
                     /gene="dmpG"
                     /locus_tag="RH058_000416"
     CDS             complement(8197..9210)
                     /gene="dmpG"
                     /locus_tag="RH058_000416"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV3136056.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9207..10157)
                     /gene="mhpF"
                     /locus_tag="RH058_000417"
     CDS             complement(9207..10157)
                     /gene="mhpF"
                     /locus_tag="RH058_000417"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV3136057.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10154..10963)
                     /gene="mhpD"
                     /locus_tag="RH058_000418"
     CDS             complement(10154..10963)
                     /gene="mhpD"
                     /locus_tag="RH058_000418"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV3136058.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10973..11839)
                     /gene="mhpC"
                     /locus_tag="RH058_000419"
     CDS             complement(10973..11839)
                     /gene="mhpC"
                     /locus_tag="RH058_000419"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV3136059.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11857..12801)
                     /gene="mhpB"
                     /locus_tag="RH058_000420"
     CDS             complement(11857..12801)
                     /gene="mhpB"
                     /locus_tag="RH058_000420"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV3136060.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12803..14467)
                     /locus_tag="RH058_000421"
     CDS             complement(12803..14467)
                     /locus_tag="RH058_000421"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV3136061.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQHNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14544..15491
                     /gene="mhpR"
                     /locus_tag="RH058_000422"
     CDS             14544..15491
                     /gene="mhpR"
                     /locus_tag="RH058_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV3136062.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15568..16650
                     /gene="lacI"
                     /locus_tag="RH058_000423"
     CDS             15568..16650
                     /gene="lacI"
                     /locus_tag="RH058_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV3136063.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16773..19847
                     /gene="lacZ"
                     /locus_tag="RH058_000424"
     CDS             16773..19847
                     /gene="lacZ"
                     /locus_tag="RH058_000424"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV3136064.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKATGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19899..21152
                     /gene="lacY"
                     /locus_tag="RH058_000425"
     CDS             19899..21152
                     /gene="lacY"
                     /locus_tag="RH058_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV3136065.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21218..21829
                     /gene="lacA"
                     /locus_tag="RH058_000426"
     CDS             21218..21829
                     /gene="lacA"
                     /locus_tag="RH058_000426"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV3136066.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(21932..23113)
                     /gene="cynX"
                     /locus_tag="RH058_000427"
     CDS             complement(21932..23113)
                     /gene="cynX"
                     /locus_tag="RH058_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV3136067.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23119..23589)
                     /gene="cynS"
                     /locus_tag="RH058_000428"
     CDS             complement(23119..23589)
                     /gene="cynS"
                     /locus_tag="RH058_000428"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV3136068.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23620..24279)
                     /gene="cynT"
                     /locus_tag="RH058_000429"
     CDS             complement(23620..24279)
                     /gene="cynT"
                     /locus_tag="RH058_000429"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV3136069.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24389..25288
                     /gene="cynR"
                     /locus_tag="RH058_000430"
     CDS             24389..25288
                     /gene="cynR"
                     /locus_tag="RH058_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV3136070.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25328..26611)
                     /gene="codA"
                     /locus_tag="RH058_000431"
     CDS             complement(25328..26611)
                     /gene="codA"
                     /locus_tag="RH058_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV3136071.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26601..27860)
                     /gene="codB"
                     /locus_tag="RH058_000432"
     CDS             complement(26601..27860)
                     /gene="codB"
                     /locus_tag="RH058_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV3136072.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(28097..29983)
                     /gene="prpE"
                     /locus_tag="RH058_000433"
     CDS             complement(28097..29983)
                     /gene="prpE"
                     /locus_tag="RH058_000433"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV3136073.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRYQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30023..31474)
                     /gene="prpD"
                     /locus_tag="RH058_000434"
     CDS             complement(30023..31474)
                     /gene="prpD"
                     /locus_tag="RH058_000434"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV3136074.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31508..32677)
                     /gene="prpC"
                     /locus_tag="RH058_000435"
     CDS             complement(31508..32677)
                     /gene="prpC"
                     /locus_tag="RH058_000435"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV3136075.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32722..33612)
                     /gene="prpB"
                     /locus_tag="RH058_000436"
     CDS             complement(32722..33612)
                     /gene="prpB"
                     /locus_tag="RH058_000436"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV3136076.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33851..35437
                     /gene="prpR"
                     /locus_tag="RH058_000437"
     CDS             33851..35437
                     /gene="prpR"
                     /locus_tag="RH058_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV3136077.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35535..35810)
                     /locus_tag="RH058_000438"
     CDS             complement(35535..35810)
                     /locus_tag="RH058_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3136078.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35957..36628
                     /locus_tag="RH058_000439"
     CDS             35957..36628
                     /locus_tag="RH058_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV3136079.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36645..36890)
                     /locus_tag="RH058_000440"
     CDS             complement(36645..36890)
                     /locus_tag="RH058_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136080.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(37302..38117)
                     /locus_tag="RH058_000441"
     CDS             complement(37302..38117)
                     /locus_tag="RH058_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136081.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38360..39409)
                     /gene="yahK"
                     /locus_tag="RH058_000442"
     CDS             complement(38360..39409)
                     /gene="yahK"
                     /locus_tag="RH058_000442"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV3136082.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39496..40452)
                     /locus_tag="RH058_000443"
     CDS             complement(39496..40452)
                     /locus_tag="RH058_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3136083.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(40449..41420)
                     /locus_tag="RH058_000444"
     CDS             complement(40449..41420)
                     /locus_tag="RH058_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3136084.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(41413..42897)
                     /locus_tag="RH058_000445"
     CDS             complement(41413..42897)
                     /locus_tag="RH058_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3136085.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42946..43929)
                     /locus_tag="RH058_000446"
     CDS             complement(42946..43929)
                     /locus_tag="RH058_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3136086.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(44183..44593)
                     /locus_tag="RH058_000447"
     CDS             complement(44183..44593)
                     /locus_tag="RH058_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3136087.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTAACGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44892..46274)
                     /locus_tag="RH058_000448"
     CDS             complement(44892..46274)
                     /locus_tag="RH058_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV3136088.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(46284..47234)
                     /locus_tag="RH058_000449"
     CDS             complement(46284..47234)
                     /locus_tag="RH058_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV3136089.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(47377..48795)
                     /locus_tag="RH058_000450"
     CDS             complement(47377..48795)
                     /locus_tag="RH058_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV3136090.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48795..50342)
                     /gene="fdrA"
                     /locus_tag="RH058_000451"
     CDS             complement(48795..50342)
                     /gene="fdrA"
                     /locus_tag="RH058_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV3136091.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(50332..51195)
                     /locus_tag="RH058_000452"
     CDS             complement(50332..51195)
                     /locus_tag="RH058_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV3136092.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(51235..51840)
                     /locus_tag="RH058_000453"
     CDS             complement(51235..51840)
                     /locus_tag="RH058_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV3136093.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            52098..52595
                     /locus_tag="RH058_000454"
     CDS             52098..52595
                     /locus_tag="RH058_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV3136094.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52687..53619
                     /locus_tag="RH058_000455"
     CDS             52687..53619
                     /locus_tag="RH058_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136095.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53661..54749)
                     /locus_tag="RH058_000456"
     CDS             complement(53661..54749)
                     /locus_tag="RH058_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3136096.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54891..58874)
                     /gene="ehaA"
                     /locus_tag="RH058_000457"
     CDS             complement(54891..58874)
                     /gene="ehaA"
                     /locus_tag="RH058_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HDV3136097.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVVVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVIVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNSDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTSMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        14090 a        16004 c        14726 g        14272 t
ORIGIN      
        1 aacatttaac atttataaat taacatctgg ctgcgccaga gattacgtct ctgagtgcat
       61 cgttgtgctt gtagtataat aaataaaatg gagtgtcatt aagtggataa ggttttagat
      121 tcagccctcc tttcttcggc aaataaaaga aagggtatcc ttgctattgg cgcacatcct
      181 gacgatatag aattaggctg tggcgcatcg cttgctcgtc ttgcgcaaaa aggaatttat
      241 atcgcagccg tggtgatgac taccggcaac tctggtacag atggaataat cgatcgccat
      301 gaagaatcgc gcaacgccct aaagatatta gggtgccacc aaactattca tcttaatttt
      361 gctgacaccc gcgctcattt acagctcaat gatatgattt ccgctctgga agacatcatt
      421 aaaaatcaaa ttccttctga tgttgaaatc atgcgggtat ataccatgca tgatgccgac
      481 cgccatcagg atcatctcgc tgtttatcaa gcttcaatgg ttgcctgccg cactattcca
      541 caaattctcg gctacgaaac cccaagtacc tggctttcat ttatgcctca ggtttttgaa
      601 tccgttaaag aagaatattt cacagtcaaa cttgctgcat taaaaaaaca taaaagccag
      661 gaacgacgtg attatatgcg tcatgatcgc ctgcgtgcag ttgcacaatt tcgcgggcaa
      721 caggtcaata gcgatctggg tgaaggcttt gtaattcata aaatgattct ttgagcaaat
      781 tgcatgaaaa cctggatatt tatctgtatg tccatagcaa tgttgctatg gtttttaagt
      841 acgctaagac gtaaacccag tcaaaagaaa ggctgtattg acgccattat acctgcgtat
      901 aacgaaggcc cgtgtctggc gcagtcactg gataatctac tgcgtaaccc ttatttttgc
      961 cgggtaattt gcgttaacga cggctccacg gacaataccg aagcggtcat ggcggaagtc
     1021 aaacgcaaat ggggcgaccg ctttgttgcc gtcacgcaaa aaaataccgg taaaggtggt
     1081 gcgctgatga atggcctcaa ttatgccacc tgcgaccagg tttttttaag tgatgccgac
     1141 acctatgttc cgcccgatca agacggaatg ggctatatgc tggcagaaat tgagcgcggt
     1201 gctgatgccg taggcggcat tccctctact gcgttgaaag gcgcggggct gttaccgcac
     1261 atccgcgcga ccgtaaagtt gccgatgatt gttatgaagc gcacgctaca gcagctcctg
     1321 ggcggcgcac cgtttattat cagcggtgcc tgcgggatgt tccgtactga tgtattgcgt
     1381 aagttcggtt tctcggatcg tactaaagtc gaagaccttg atctcacctg gacattagtg
     1441 gcaaacggct accgtattcg gcaggcgaat cgctgcatcg tatacccaca ggaatgcaac
     1501 agcccgcgtg aggagtggcg tcgctggcgg cgttggattg tgggatacgc ggtctgtatg
     1561 cgcctgcata aaagactttt atttagccgc ttcggtatct tcagtatatt tcctatgctg
     1621 ttggttgtga tttatggcgt tgggatttat ctcactacct ggtttaatga attcatcacc
     1681 accgggccgc atggtgtggt gttggcaatg tttccgctta tctggatcgg cgtagtttgt
     1741 gttattggtg cttttagcgc ctggtttcat cgttgctggt tgttggtgcc tttagcgccg
     1801 ctttccgttg tgtatgtatt attagcttat gccatctgga ttatttatgg acttattgcc
     1861 ttttttactg gacgcgaacc tcagcgcgac aaacccaccc gctattccgc actggtggaa
     1921 gcgtcaaccg cttattccca accttctgtc acaggaactg aaaaactatc tgaagcttaa
     1981 tgttaaagag aaaaatattc agattgcaga ccaggtaatt attgatgaaa ctgcaggtga
     2041 agttgttatc ggcgcgaata cccgtatttg tcatggtgcc gttattcagg gtccggtagt
     2101 gattggcgca aactgcctga taggtaatta tgcgtttatt cgtcctggca caataatcag
     2161 caatggtgta aaaattggtt ttgccaccga aattaaaaat gcggttattg aagcggaagc
     2221 aacgattggt ccgcaatgtt ttattgccga ctcggtagtt gcaaaccagg catatttggg
     2281 cgcacaagta cgtaccagta atcatcgtct ggatgaacag cctgtgtctg ttcgaactcc
     2341 agagggaatt atcgctaccg gatgcgataa attaggttgt tatatcgggc agcgttcacg
     2401 ccttggcgta caagttataa ttttgcctgg gcgaattatt tccccgaaca cgcaacttgg
     2461 cccgcgcgtg attgtagaac gtaatttacc tgccggaact tattcactcc gacaagaact
     2521 tatccgtaca ggagattaaa atgataaaac ggacgttatt agcggcggcc atttttagcg
     2581 cattgcccgc ttatgccggg ttaacttcca ttaccgcggg ctacgatttt accgattatt
     2641 ctggcgatca tggcaaccgt aatttagcgt atgctgaact ggtggcgaaa gttgaaaacg
     2701 caacgctgct ttttaatctt tcccaggggc gtcgtgatta tgaaactgaa catttcaatg
     2761 ccactcgcgg tcagggtgcg gtctggtata aatggaataa ctggctgaca acccgaacgg
     2821 gtattgcctt tgcggataat acgccggtct ttgcccgcca ggattttcgt caggatatta
     2881 acctggccct gttgccaaaa acgcttttca cgaccggtta tcgctacact aaatattacg
     2941 atgatgtcga agtcgatgcc tggcaaggcg gcgtatcact ctatactggc ccggtgatca
     3001 ccagctaccg ctatacccat tatgactcca gcgatgcagg tggtagttat agcaatatga
     3061 tttccgtgcg tctgaatgac ccgcgcggca ctggttatac gcaactatgg ctaagccgcg
     3121 gaacaggtgc ttacacctat gactggacgc cagaaacacg ctacggcagc atgaagagcg
     3181 tcagtctgca acgtattcaa ccgctgactg agcaacttaa tctcgggctg acggcaggta
     3241 aagtgtggta cgacacccca accgatgatt ataacggtct gcaacttgca gcccatctga
     3301 cctggaaatt ctgattcctt ctgccgcccg ctatccgggg cggccttccc tgccgattag
     3361 cccccccttt cctctttgtt ttccgaccac attcaccgga taaattttat tctccagtgt
     3421 tatatactat aggggggtat gcattgacat atagcatacc cccctatagt atattgcgtg
     3481 cagatgatga ggtgcgaaat gcccagtact ccggaagaga agaaaaaggt ccttactcga
     3541 gttcgtcgta ttcgagggca gattgatgct ctggaacggt cgctggaggg tgatgccgaa
     3601 tgccgtgcca tactccaaca gatcgctgcc gttcggggcg cggctaacgg gctgatggca
     3661 gaagtgcttg aaagccatat ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa
     3721 gtcagccaat ccgttgacga cactattgaa ctggttcgag cctatcttaa atagctgaat
     3781 ctattaccat attgaggaag agcgagagat gaaatcacgt gctgccgttg catttgctcc
     3841 cggtaaaccg ctggaaatcg ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct
     3901 gattaaagtc acccataccg gcgtttgcca taccgacgca tttaccctct ccggggatga
     3961 cccggaaggt gtattcccgg tggttctcgg tcacgaaggg gccggcgttg tggttgaagt
     4021 cggtgaaggc gtaaccagcg tcaaacctgg cgaccatgtg atcccgcttt acaccgcgga
     4081 gtgcggcgag tgtgagttct gtcgttctgg caaaactaac ctctgtgttg cggttcgcga
     4141 aacccagggt aaaggcttga tgccagacgg caccacccgt ttttcttaca acgggcagcc
     4201 gctttatcac tacatggggt gctctacatt cagtgaatac accgtggtcg cggaagtgtc
     4261 tctggccaaa attaatccag aagcaaacca tgaacacgtc tgtctgctgg gctgtggcgt
     4321 gaccaccggt attggcgcgg tgcacaacac agctaaagtc cagccaggtg attctgttgc
     4381 cgtgtttggt cttggcgcga ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc
     4441 gggacggatt atcgctatcg ataccaaccc gaagaaattc gatctggcgc gtcgcttcgg
     4501 tgctaccgac tgcattaacc cgaatgacta cgacaaaccg attaaagatg tcctgctgga
     4561 tatcaacaaa tggggtatcg accatacctt tgaatgcatc ggtaacgtca acgtgatgcg
     4621 tgcggcgctg gaaagtgcgc accgcggctg gggtcagtcg gtgatcatcg gggtggcagg
     4681 ttctggtcag gaaatctcca cccgtccatt ccagttggtc actggtcgcg tatggaaagg
     4741 ttccgcgttt ggcggcgtga aaggtcgttc ccagttaccg ggtatggttg aagatgcgat
     4801 gaaaggtgat atcgatctgg aaccgtttgt cacgcatacc atgagccttg atgaaattaa
     4861 tgacgccttc gacctgatgc atgaaggcaa atccattcga accgtaattc gttactgatt
     4921 tcccgcaggt ataccccgtc cacttcagac ggggttctta atactctccc tgggcagccg
     4981 tccgggggat taaccgtgag ataatgactg atggaactca ttgaaaaaca tgccagcttt
     5041 ggcggctggc aaaatgtgta tcggcattat tcccaatcac tgaaatgtga aatgaatgtc
     5101 ggcgtctatc tcccaccaaa agccgcgaat gaaaaattgc cggtgctgta ctggctttcg
     5161 ggcctgacct gcaacgagca gaatttcatt actaaatcgg ggatgcagcg ttacgcggct
     5221 gaacacaaca ttattgttgt tgcgccggac accagtccgc gaggcagtca tgtcgcagat
     5281 gccgaccgtt acgatctcgg gcaaggtgcc gggttttacc tgaacgcaac gcaagcgccg
     5341 tggaatgaac attacaaaat gtatgactat atccgcaacg agctgccgga tttagtgatg
     5401 caacattttc cggcaacgac cagaaagtct atctctggtc attctatggg cgggctgggc
     5461 gcgctggtgc tggcgttacg taacccagat gaatatgtca gcgtctcggc gttttcgccc
     5521 attgtctccc catcgcaagt accgtgggga cagcaagcct ttgccgcata tcttggtgaa
     5581 aataaagatg cctggttgga ttacgatccg gtgagtctta tttcacaagg tcaacgcgtt
     5641 gcggaaatca tggttgatca ggggttgagt gatgattttt acgcggaaca gctgcggact
     5701 ccaaatcttg aaaagatctg ccaggagatg aatatcaaga cgttaatccg ttatcacgag
     5761 ggttacgacc acagctatta ttttgtctcc agttttattg gcgagcatat tgcctaccac
     5821 gccaataaac tgaatatgcg ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc
     5881 ttatccggcc tacacttcgc ccgtaaaccg taggcctgat aagacgcgca aagcgtcgca
     5941 tcaggcatgg cgcacgactg ccggatgcgg cgtgaacgcc ttatccggcc tacacttcgc
     6001 ccataaaccg taggcctgat aagacgcgca tagcgtcgca tcaggcatct gcgcacgact
     6061 tatctatgtt taccacatca aatcatcagg cactttgaag tcggcatacg gatcgtcttc
     6121 atcctgctct tcggcactga gcgcgctgtg caacacaata ctgctggcat cacgctgagc
     6181 aattttatcg gcaacgctgg cgggaataat ggcgtattca ccttcgctat tgttatcgac
     6241 caacaagcgg gcaatcgcca gacgaccatt aatcaactgc gcctgtgtga gcttatcgac
     6301 aaaaatcttc ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc cattggcaat
     6361 ggtgattcgg ttcatttcaa tgagctgctt cacctgagct ttatattctt tcgccaacgc
     6421 cgcttgtttt tgctgttcgc taagctgttt atcacgctca agctgtgcct ttttattttc
     6481 ttctaccgcc gcccgagctt cacgcgcctg aacgcgcgat tttttcgccg ttctctcgac
     6541 cttcgccgct tttttgctgg tgactaatcc tgctttgagc aactgctctt gtaaggtaag
     6601 ttttgccatc tttgtttcca aacccgctga ataattttta gcgagtatac ctgtaaacgc
     6661 cgtgggtgta ttgggggcaa gtccctttcg cccctgctta ataaggcgcg tcaggcatcg
     6721 gcacacggct gcattcttga tctccgactc atcaaaataa acaccgccaa cccagcaaca
     6781 agaatacccg gtgcagaagc ggccattacg ccgaccgtgc cagtgcctaa tgccagcatt
     6841 ttcccggcca gtaacggacc gctcatagcc cccagacgcc ctaccgccac ggctgttccc
     6901 acacctgttg cgcggatctg cgaactgtaa aacaacggtg ccagggcata caaaacgctt
     6961 tgtccacctg tcgcaaacaa ccccgcgaca aatcccgcca gcaacatacc gttaaatgac
     7021 gacaccgttc caagcgccag cagcgaagct aacatgccgc tataaatcag tagcgacatg
     7081 gttactggac gcagcttatc catcaatgcg cccaacatta acgtcccgct tgccgccccc
     7141 atttgcaggg caaacatcac ccctgccgcc tgcgatggct ggaatccttg ctccaccaaa
     7201 agtagcggta gccagttgat caacatataa accaccagca gagtgaagaa ataacacaac
     7261 cacagcagca gcgtcgcggt tgccgtttct ggcgcaaata aggcacgcag tggtggcgca
     7321 gcctgttttt cgccagcgaa aaccgctgac tccggcagcc agcgcattaa tagcggcacc
     7381 agaatcaacg gcaccacacc acctacccaa aacaccgttt gccatgctaa gtttgccccc
     7441 gcgaaaccca gtgtcgccgc cagcgccgcg ccaatgggaa caccgcaata catcaggctc
     7501 actgctgtcc cacgaaaacg tggacccgcg gcttcagacg tcagggcgat aagattcggc
     7561 aacgccgccc ccagcccgac accggtcatc agccgcgcaa agaccagtga ggggaaatcc
     7621 caggcaatcg ccgttgccag cgagaacaaa ccaaacagcg caactgagcc aatcaaaatg
     7681 cgcttgcgac cataacggtc cgccagcatt ccgccaacca acgcgccggg tagcaaaccg
     7741 agtattccgg cgctaaatat ccagcccatt tgcattttat cgagtgcgaa agcctgggcg
     7801 atgccacccg ccgcaatgcc agccgcctga agatccagcc cttccatcag agcgaccaaa
     7861 aaacaaagcc cgatagtcag catcaggcgg gatgaagatg atgaaggggt acgagtcgac
     7921 ataagatgaa cctcgttgcg agtgcaggtg aatcgcgact gatacattgc cagccgcgta
     7981 atcagaacta tttaatgact gcctacgggc gaaatgtagg ccggacaaga cgtttatcgc
     8041 atccggcagt cgtgtgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
     8101 ttctcacgcc acccgtaggc cggataaggt gtttacgccg catccggcag tcgtgctggg
     8161 atgggcatac aaaatgcacg ttctcggcag tcatcattat ttgttgttgc gcagatccag
     8221 cgccacgtca acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac
     8281 gagaatatcc accgcactta agccataacg cgccgccgcc gtttcacagt gacgcaggaa
     8341 gctcgagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
     8401 atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcggtccc
     8461 atgctgccag cccagtttat ccgcggcggc aataaatact tccagcggcg cgttacctgc
     8521 gcccgcgccc attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
     8581 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccag tttgcgtttc
     8641 tggtttcagc tctgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
     8701 accgccagaa tccaccacat aaatacaggt cgcaccgtag ccttccatca gctttgcctg
     8761 ctttgcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
     8821 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
     8881 tgccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
     8941 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
     9001 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggaac cttgcaaacc
     9061 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
     9121 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaatgtgac
     9181 gtccgagata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
     9241 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatatcgag gttgcccgca
     9301 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
     9361 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
     9421 acgcgctgtt tcaggcgata acccggtacg taagcctgca ccgcctcagc catttcattg
     9481 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
     9541 atcatcaacg gtggctctgc tgggttaagc acaataatcg ccttcccttt tgccgcgccg
     9601 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
     9661 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
     9721 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatgttg
     9781 acgttcagtt gatcgacgtt cgcctcgagg ttaaccaccg gcacgcagta agggccgatg
     9841 gcagcaggcg tcaggtcaat taagcgaata tccggtttcg cttcgcgtaa agcggcatcg
     9901 tttttcacat gcgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
     9961 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
    10021 cgcgccagac cgtcggactg aggatcaatg ccgaccatca ccgccatctc cagatgctga
    10081 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
    10141 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
    10201 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact gccaccatcg
    10261 gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc aggctggcca
    10321 ttttgcgtgc cagccagacg gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc
    10381 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
    10441 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
    10501 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct ccccaccact tccagcgccg
    10561 gaagtaccca ttcaatggcg ttatacagtt cgtcgaaggt gatatcagtt gcgggtaaat
    10621 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
    10681 gaatgatttc gttatcgcca taacacatgt cggcaaataa cgtcccaaaa tccggttgat
    10741 caacgcccag ttgttgttgc actttcggat gcgtcaggcc cactttacgc cctaccacgc
    10801 gacgcccctg cacaacgtca tattgcacat ttatgtgctg aatggcgtag gccgcttcag
    10861 cgttatcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc
    10921 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccatt ccttaagggc
    10981 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccagt
    11041 gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
    11101 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
    11161 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa
    11221 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
    11281 cgtcggtcaa atcgctggta tcgaaaacga agatatccat catcagcttc aggttttcga
    11341 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
    11401 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
    11461 agttaagggt gaacgccaca gaactatggc cgcccatcga gttgcccagc aggtggattt
    11521 tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgtg
    11581 aaccactatt aacgatcgaa tcgcttttgc cccaacccgg acaatccagc aggattaccc
    11641 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac
    11701 ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
    11761 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
    11821 tttgtggctg ataactcatc atcttctcct gcatattcag ttctctgttc tggcgcttaa
    11881 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
    11941 gttgccaaat gtagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
    12001 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
    12061 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
    12121 ctgatcctca acaaacttct cagcggcgct aatcacccgt tgctgacgca attcgcgctc
    12181 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
    12241 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
    12301 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
    12361 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
    12421 cccaccgagc aggaactcca gcggctgggc gaacccgtgg tccacctgca tacagtaaga
    12481 aacggcaaga tcgatcccgc tcttcatgac agcatgcgca caggcctccg ccagctccac
    12541 aggcacgggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
    12601 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
    12661 caccaccagt tcaggggaga atgccgcaat acgctcgcgg gcgctggcaa tcacgccatt
    12721 gacctcatcg agcacctctt gcgccgggtc gacatacccc accagcggcg agtgggaaag
    12781 acagtgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
    12841 gagtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
    12901 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga
    12961 accagctacg caggcgacct tgcgtatcgc ccacgcgtag tacgccgtcg tggttatcct
    13021 gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc
    13081 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
    13141 cgaagttcgc gccgatcgcg ttatcgagca gcgtcacgtc gccgttttcc agcgtgactt
    13201 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgcatcagcg
    13261 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
    13321 gcggcagata attcaacagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
    13381 gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
    13441 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
    13501 ccagtttcca tgcgaggtta aaggcgtcgc gcataccact gttatagccc tgcccctgcc
    13561 ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
    13621 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
    13681 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgctcttcgg
    13741 tttctcccgg catcaccata aattcaaagc gacgtaccgc atgaggcagc gcggcagaaa
    13801 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
    13861 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcagag
    13921 tgcgacggac aaaacttgct ccaccgtcac aggctaccag ccactgggct ttgaccgttt
    13981 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcattgtgc tggctgaagg
    14041 cctccagctc gcgggcaaac aagcagcgca cattcggaaa acgcgacaac ccttccagca
    14101 tcaccgcatc gacctgtggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
    14161 tcattggctg aatatcagca aaacagcggc cttttggggt gagaaaacgc atcgcgtgcc
    14221 acggtgtagt gtgcggcaga acattctcga ccaggccgac cgactgcatg gtgcgcagcg
    14281 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
    14341 ccagcacgtc aatgcccatc tgaccgagat agttcgccat catcagccca accggaccgg
    14401 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
    14461 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
    14521 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata ggaagggtat
    14581 tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta
    14641 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
    14701 taacacgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt gccagcgtcg
    14761 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
    14821 tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt ctgaccatca
    14881 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
    14941 cgctgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
    15001 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc tttcaccggg
    15061 caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc tggctggcct
    15121 tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc cccggtgatg
    15181 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc gcgcgcaaag
    15241 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
    15301 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
    15361 caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg gtagcaaaac
    15421 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
    15481 atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg atagcgcccg
    15541 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga tgtcgcagag
    15601 tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag ccacgtttct
    15661 gcgaaaacgc gagaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
    15721 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
    15781 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
    15841 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
    15901 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
    15961 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
    16021 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
    16081 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
    16141 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
    16201 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
    16261 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
    16321 ggcgcaatgc gcgccattac cgagtccgga ctgcgcgttg gtgcggatat ctcggtagtg
    16381 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
    16441 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
    16501 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
    16561 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    16621 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    16681 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtgaaattgt
    16741 gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg
    16801 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    16861 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    16921 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg
    16981 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa
    17041 actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg
    17101 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg
    17161 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg
    17221 cgtttcatct gtggtgcaac ggtcgctggg tcggttacgg tcaggacagt cgtttgccgt
    17281 ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc
    17341 tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt
    17401 tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca
    17461 ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg
    17521 agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca
    17581 gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtagttat gccgatcgcg
    17641 tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct
    17701 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg
    17761 atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt
    17821 tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg
    17881 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct
    17941 gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg
    18001 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg
    18061 atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta
    18121 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg
    18181 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg
    18241 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag
    18301 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag
    18361 agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggct
    18421 tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc ttcgtctggg
    18481 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg
    18541 gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg
    18601 ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt
    18661 tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata
    18721 acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc
    18781 ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccag
    18841 agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt
    18901 cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga
    18961 cgctcccctc cgcgtcccat atcatcccgc aactaaccac cagcgaaacg gatttttgca
    19021 tcgagctggg taataagcgt tggcaattta accgtcagtc aggccttctt tcacagatgt
    19081 ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgcgcaccgc
    19141 tggataacga cattggcgta agtgaagcga cccgtattga ccctaacgcc tgggtcgaac
    19201 gctggaaggc gacgggccat taccaggccg aagcggcgtt gttgcagtgc acggcagata
    19261 cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag ggaaaaacct
    19321 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg
    19381 atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc tgccagctgg
    19441 cgcaggtcgc agaacgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc
    19501 gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt
    19561 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
    19621 accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa caactgatgg
    19681 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
    19741 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc
    19801 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca
    19861 ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa
    19921 acttttggat gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc
    19981 cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat acgggtatta
    20041 tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg
    20101 acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta gtgatgtttg
    20161 cgccgttctt tatttttatc ttcgggccac tgttacaata caacatttta gtaggatcga
    20221 ttgttggtgg tatttatcta ggcttttgtt ttaacgccgg tgcgccagca gtagaggcat
    20281 ttattgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg atgtttggct
    20341 gtgttggctg ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc aataatcagt
    20401 ttgttttctg gctgggttct ggctgtgcac tcatcctcgc cgttttactc tttttcgcca
    20461 aaacggatgc gccctcttcc gccacggttg ccaatgcggt aggtgccaac cattcggcat
    20521 ttagccttaa gctggcgctg gaactgttca gacagccaaa actgtggttt ttgtcactgt
    20581 atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct aatttcttta
    20641 cttcgttctt tgctaccggt gaacagggta cgcgggtatt tggctacgta acgacaatgg
    20701 gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat cgcatcggtg
    20761 ggaaaaacgc cctgctgctg gctggcacta ttatgtctgt acgtattatt ggctcatcgt
    20821 tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt gaagtaccgt
    20881 tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt ttttcagcga
    20941 cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt atgtctgtac
    21001 tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg ctgggtctgg
    21061 tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc ccgctttctc
    21121 tactgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc ggcgcgagcg
    21181 ccttatccga ccaacatatc aggacggaat gatcgcaatg aacatgtcga tgacagaaag
    21241 aataaaagca ggcaagctat ttaccgatat gtgcgaaggc ttaccggaaa aaagacttcg
    21301 tgggaaaacg ttaatgtatg agtttaatca ctcgcatcca tcagaagttg aaaaaagaga
    21361 aagcctgatt aaagaaatgt ttgccacggt aggggaaaac gcctggatag aaccacctgt
    21421 ctatttctct tacggttcca acatccatat aggccgcaat ttttatgcaa atttcaattt
    21481 aaccattgtc gacgactaca cggtaacgat cggtgataac gtattgattg cacccaatgt
    21541 tactctttcc gttacgggac accctgtaca ccatgaattg agaaaaaacg gtgagatgta
    21601 ctcttttccg ataacgattg gcaataacgt ctggatcgga agtcatgtgg ttattaatcc
    21661 aggcgtcacc atcggggata attctgttat tggcgcgggt agtgtcgtca taaaagacat
    21721 tccaccaaac gtcgtggctg ctggcgttcc ttgccgggtt attcgcgaaa taaacgaccg
    21781 ggataagcaa tattatttca gagattacaa agttgaatcg tcagtttaaa ttataaaaat
    21841 tgcctgatac gcttcgctta tcaggcctac aaattcagtg atctacgtag gccgcatccg
    21901 gcgtgaacaa agcgcagaaa caagagtcgc atcatgcctc tttgacccac agctgcggaa
    21961 aacgtgctgg tgcaaaacgc agggttataa tcatcagccc aacgacacac agcgcatgaa
    22021 acgcccagtc catcaggtaa ttgccgctga tactacgcag cacaccagaa aaccaagggg
    22081 caagcccggc gatgataaaa ccgatcccct gcataaacgc caccagcttg ccagcaatag
    22141 ctggttgcgc agagtgatcg agcgccagca gcaaacagag cggaaacgcg ccgcccagac
    22201 ctaacccaca caccatcgcc cacaataccg gcaattgcag cggcagccag ataaagccgc
    22261 agaaccccac cagttgtaac accagcgcca gcattaacag tttgcgccga tcctgatggc
    22321 gagccatagc aggcatcagc aaagctcctg cggcttgccc aagcgtcatc aatgccagta
    22381 aggaaccgct gtactgcgcg ctggcaccaa tctcaatata gaaagcgggt aaccaggcaa
    22441 tcaggctggc gtaaccgccg ttaatcagac cgaagtaaac acccagcgtc cacgcgcggg
    22501 gagtgaatac cacgcgaacc ggagtggttg ttgtcttgtg ggaagaggcg acctcgcggg
    22561 cgctttgcca ccaccaggca aagagcgcaa caacggcagg cagcgcccac caggcgagtg
    22621 tttgatacca ggtttcgcta tgttgaacta accagggcgt tatggcggca ccaagtccac
    22681 cgccgcccat cagagccgca gaccacagcc ccatcaccag tggcgtgcgt tgctgaaacc
    22741 gccgtttaat cactgaaggc atcaccgcct gaatgatacc gatccccacc ccaccaagca
    22801 gtgcgctgct aagcagcagc acactttggg ggtaaagctc acgcatcaat gcaccgacgg
    22861 caatcagcaa cagactgatg gcgacgctgc gacgttcgct gacatgctga tgaagccagc
    22921 ttccggccag cgccagcccg cccatggtga ccaccggcag agcggtcaac agggcagcca
    22981 cgctaaagct cattccgctc gcctggcgca attgcggtag cagtggaccg acggaggtga
    23041 gcagtggtcg catattaaga ccaatcagca ccagaaccag cagcatcgtg aacgtctttt
    23101 gatggttgcg catggctgtc agaacggttt ggtcggcaga tatttaccat ctaaggtgat
    23161 gaccgcacgt tcgccacctt ccgggtccgc cactttctta acgtcgagtt tgaagttaat
    23221 cgcgctgata atgccatcgc caaacttctc atgaaccaac gctttcaggg ttgtgccgta
    23281 cacctgcaac atttcataga aacgatacat cgttgggtcg gttggaatac ggtcatcaat
    23341 gcagccacgc agtggaatca tctgcaacag tagaatggcg tcttcgtcga gatccagctt
    23401 cgccccgacc aggcgggcgg cgtcggcagg aagcgcctgc tgccccagca aagccgcggt
    23461 tacaaaggct tctgccagac cggtgccgtc ggcaatctcg gcaaatgaga gatctttttt
    23521 agctttgctg agcaaaatgg catcggcaag atcaagacga atattgcggt taatttgcga
    23581 ctgaatcatg gtgcatctcc tgatggttta aaaataaggt tacgctgcgg tcggttggcg
    23641 tagcggtatg gcacaaacgc gcggattagc tgccagtggc acaaactggc gggttgcgcc
    23701 gtcaaacgct gcgatgctgc cgctttcaat gtcgtagacc cagccgtgca gggcgatccg
    23761 cccctcttcg agcgccaggc gcaccgatgg atgagtttgc aaattagcca actgagcaat
    23821 gacgttttca cgtaccatcg ccgcagcttt tgacggtaaa tcggaatgcg ggcgcgcctc
    23881 attaacgacg cgggctgaat cggcataacg cagccagtgg gagacggcag gcatatggtc
    23941 catgcactga cagctggcaa tggcggtcat cgcgccacag ttggaatgac cacaaatcac
    24001 aatgtcagat acccgaagcg cagcgacggc atactccacc gaagcagaaa cgccaccggg
    24061 ttccggcccg taggaaggga cgatattgcc cgcgttgcga ataacgaaca gatcgccagg
    24121 ctcacgttgc gtcaccagct cagggaccag acggctgtcg gagcaggaga taaaaagtgt
    24181 gcgcgggctt tgctgtgtcg ccagctgttt aaacaaggct tcccgcttcg gaaatgcctc
    24241 gcgctggaat ttaaggaatc catcaataat ctctttcacc gttaacctct gtctgtctct
    24301 ggaatgagag gcagactact cgccgattgt cataaggtaa aagtctcatt tatgatgagt
    24361 tctattggat ttacttatag gttggcgaat gctctctcga catatcaatt actttcttgc
    24421 cgtggctgaa catggcagct tcacccgtgc cgccagtgcg ttgcacgtct cccagcctgc
    24481 gctttcccag cagattcgcc agttagagga gagtttaggc gtgccgctgt ttgaccgtag
    24541 cgggcgaacg attcgtctca ctgatgcagg agaagtctgg cgacagtacg ccagccgggc
    24601 gttacaggaa ctgggggcgg gtaaacgggc gattcatgat gttgccgatc tgacgcgagg
    24661 atcgctgcgt atcgccgtca ctcccacctt tacaagctac tttatcggcc ctttaatggc
    24721 ggatttctat gcgcgctacc ccggcatcac gctccagcta caggaaatgt cgcaggagaa
    24781 aatcgaagat ctgctttgtc gcgacgagct ggacgttggg attgccttcg cgcctgtgca
    24841 ttcgccggag ctggaggcaa ttcctttact gacagaaagt ttagcgttag tcgtggcgca
    24901 acatcatccg ctggctgcct gtgaacaggt ggcgttgagt cgcttgcatg atgaaaaact
    24961 ggtcctgctc agcgcggaat ttgccaccag agagcaaatt gaccactact gcgagaaagc
    25021 ggggctacat ccacaggtgg tcattgaggc gaactcaatt agcgcggttc tggagctgat
    25081 tcgccgcact tccctttcca cattgttacc agcagcgatt gccacacaac atgacgggct
    25141 taaagcgatt tctcttgccc cgccactact ggagagaacg gcggttttgt tgcggcggaa
    25201 aaatagctgg cagacagccg ccgcgaaggc atttttgcac atggcgttgg aggaatgtgc
    25261 ggatgttggc gagaatgaat cacggtagcc actgacacag gtcagtggct cttcaacaca
    25321 gaggaaatta ccgtttgtaa tcgatggctt ctggctgctc cagatatacg gtggtttgtg
    25381 ccggttgtgt actggcaatc accttgccgc cacgtaccga ataacgtacc ggaacctgac
    25441 ggcgcagcgc atcaaatcca ttttcagccg gcaggataat caggtttgcg ctgtttccgg
    25501 cggcaatgcc gtaatcctgc aaattcaatg ttctggcact gtggtgggtg attaaattca
    25561 ggccatcgtt aatctgcccg tagcccatca actggcaaac atgcagcccc atatgcagca
    25621 cttgcagcat attcgccgtt cccagcggat accacggatc gaagacatca tcgtgaccaa
    25681 agcagacgtt aatgccggac tccagcatct ctttaacgcg cgtgatgccg cgacgttttg
    25741 gatacgtatc gaaacgtcct tgcagatgaa tattgaccag cgggttggcg acaaagttaa
    25801 taccggacat tttcagcaag cggaacaggc gtgaggtata cgccccgtta taggagtgca
    25861 ttgccgtggt gtggctggcg gtgactcgcg cgcccatgcc ttcacggtgc gccagggcag
    25921 caacggtttc gacaaagcgc gactgctcgt catcgatctc atcacagtga acgtcgatga
    25981 gacggtcgta tttttgcgcc agggcgaagg ttttatgcag cgactccacg ccgtattcac
    26041 gggtaaattc aaaatgcgga atcgccccca ctacatctgc ccctaagcgt aacgcctctt
    26101 ccagcaacgc ttcaccgttg ggatacgaca aaatcccttc ctgagggaag gcgacaattt
    26161 gcagatcaat ccacggcgcg acttcctgct tcacttccag cattgctttc agcgcagtta
    26221 gcgttgcatc cgaaacatcg acatgggtac gcacatgctg aatgccgttg gcaatctgcc
    26281 atttcagcgt ttgccaggcg cgttgtttca catcgtcatg ggttaataac gctttgcgct
    26341 cggcccagcg ttcaatgcct tcaaacagcg tgccggactg attccagttc ggttgtccgg
    26401 cggtttgcgt ggtgtccagg tgaatatgcg gctccacaaa cggcggtata actaaacctt
    26461 gttcggcatc caggctgttt tcagttatgg gcatcacgcc ggattgcgca tcaatggcgc
    26521 tgatttttcc gtcctgcaga tgaatctgcc acagcccctc tttgcctggt aaccgggcgt
    26581 taataattgt ttgtaaagcg ttattcgaca ctgttaacct ccacatgcgt cattgctgct
    26641 gtcgttttac gattcaaaat cgggttaagg atcagatagc tcagcgcgcc acctaatacc
    26701 gcgttgaccg gaacaattcc cggtaaccag tggcctgcgg caatccccag cgcgaccgcc
    26761 agaatcgcca cccaattgac actcatcata cgcgtggtcg caaagtgctc atagcggcga
    26821 cggttcatca gatagtcggc gatgatcacg ccccctaccg gaggaatagc tgccgaaagg
    26881 aaggtcaacc agccgacaaa attgttatac agccataatg cgcagaccgt accgataata
    26941 ccgttgatta ccgaaagggt tttgctcgac ataccggtaa tgttggcgaa acctaaaccc
    27001 gacgcataga gtgcgttatc gttggtggtc cagatattca gccccagcac cacaatcgca
    27061 ggcagcagca ggccctgagc aatcatcaca tcagagatat ccgccatgcc cagtgccgca
    27121 gcgcccgctg caccgaaaat aaacatcaac gagttgccga ggaaaaaggc caccatcgcc
    27181 accagcaccg ccagtttggc attgcgacca aaccggacaa agtcagcggt gagcgtaccc
    27241 gcactgataa atgaccccac aaccagcgcc agcgcgacat tgaaatctaa cggttgtgcg
    27301 ggaacgaccg cttttaatgc gtccaggccg cccatgccgt taacagccag ccacacggaa
    27361 taaccgccca ggcaggcgat agccggaacc gcaatcaccg aaagaaccgt cagcgccgaa
    27421 atgccaaaaa agacggtgac ggtcatcagt aaaccggaaa cggcaatcag caaattaata
    27481 tccagcccgg ttgccttacc caccggaatg gcaaacatcg ccacaccgac gccaaaccag
    27541 ccaacctgag ttccgcccag tagcagtgaa ggcagccatg agcctttaac accaaacgag
    27601 aagcgagcaa gaagatgagt ggtcaggccg gtttttgcgc caatgtaacc gagaaatgaa
    27661 gtgtaaatac cgaggagaag attaccgatg agaactgcga ggaagaaatc atgatagcta
    27721 agaccggttc cgagagtgcc gccggtccac atactggcgg aaaagaaggt taatcccagc
    27781 atgacgaacg tcaatgccaa tacccctttc cgcgccgact gtgggactgg cccctggcta
    27841 aagttgttat cttgcgacac gaaattcccc ctttttgctg tttgaaaccc aaaaaatccg
    27901 ccgcattcta ttcatctgaa gataaaaagc aatcgttttc gtggggaaat atatttttta
    27961 tatgaatgag tgcaaaaagg tgggggaaaa attatcggtg taaaaacagt gggtaggcat
    28021 aataagacgc ggtaagcgtc gcatcaggca tcggcgcatg gtgtcggatg cgccacgcga
    28081 cgcatccgac aataccttac tcttccatcg cctggcggat ctgatccaat gacgcaggat
    28141 catcaatggt cgtcagatcc ccaggatcgc gtccttcgca aattgcctgg atcgtgcggc
    28201 gcagcatttt tccggatcgc gttttcggca attgcgagac aaaccagacg tgcgccgggc
    28261 gaccaaagtt gccaatctgg ctgtccacca gcgccataat cgccttctct tgcgaatgcg
    28321 ccacatcacg atcttccaga ctatcgctct ctttcggaat gacaaacgcc accgccacct
    28381 gccctttcag cgcatctttc accccaacca ccgccacttc ggcaacgccc ggatggctgg
    28441 agatactctc ttcaatctcg cgcgtgccca gacgatgccc ggcaacgtta attacatcgt
    28501 cagtgcgccc gagaataaag tgataaccgt cagcgtcacg gatgccccag tcaaaggtgg
    28561 cgtacaccgg gcgggaaaac agcgaccagt aagtcttcac aaagcggtcg tcgtcgcccc
    28621 agatggtctg aatacagccc ggcggcagcg gcccttccac caccagcatc cctttctcgt
    28681 tgacgccaca cggttcgccg gtgacttcat taagcaactg cacgttatag ccatacatcg
    28741 gcacgccggg gcttcccaga cgcgtcggcc tgtcgtccag accgcgagca atcgccataa
    28801 tcggccagcc ggattcggtc tgccagtagt tgtcgatgac cggcacatcc agcgtattgc
    28861 tcacccaact ggcggtcggc tcgtccagcg gttctccagc cagatagagc acttccagcg
    28921 acgagagatc gtgtttgcga atttcagcgg tagggaattt tttcagcacg cgaatggcgg
    28981 tcggcgctga gaacatccgg ctaacctgat atttctcgac gattttccac cacacgccgc
    29041 agtccggcca ggtcggcaat ccttcgtaaa cgatagtcgc catccccgcc agcagcggcg
    29101 cgtaaacgat atacgaatgc cccaccaccc agccgatatc cgatgcgcaa aagaacacgc
    29161 tgcccgcttt gccgccaaaa atggtgtcca tcgaggtcgc cagcgccacc gcatatccgc
    29221 cgacgtcacg ctgcacgcct ttaggtttgc cggtcgtgcc ggaagtgtag agaatgcagg
    29281 aggtttcgtt ggattccagc cacgccactg gtacccgcgc gccgatgtgt tgatagcgca
    29341 acgacgcgaa atcgacatcc cgcccgctga cgcgcgccat tttcgccagt ccgcgatcca
    29401 ccagcaaaac atggcgtggc tggtgctgcg cctgacttat cgcatcgtcg agcaattttt
    29461 tatagggaat gattttgcca ccgcgcgctc cggcatcagc cgaaacaatc agcaccggtt
    29521 tagcatcatc aattcgcgcc gccacgctgt gcgaggcaaa tccaccaaac accaccgagt
    29581 gaatagcgcc aatgcgcgcg caggccagca gagtaatatg cgcttcggca atcatcggca
    29641 tatacaccag cacccgatcg ccacgctgca cgcccaatga acgcaacatt gacgccaccg
    29701 cgtttacttc gtcatgaagc tgacgaaagg taaaggtgcg ctcttcttct gtttccgaag
    29761 agacggcaat cagcgccagc gcctctggtt gtttctccag ccagcggtcg atagcgttgt
    29821 ggcacaagtt ggttcggcct tcacaaaacc aacgggcaaa cggcggattg ctgtggtcga
    29881 gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc ctgctcggcc cagaactgct
    29941 ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga cataatgctc tcctgttgaa
    30001 cttacgcctt accgccgttt acttaaatga cgtacaggtc gagatactca ttgaccggca
    30061 tctgttccag gcgagttctg tcgagagaaa cctccagaat gcgctgctgt tggcgagtcg
    30121 ggaactggcg cgcgagattg attttgaatt tatcgaccag tttcggaata ccatcctgac
    30181 ggcggcgagc atgaccaatg gggtactcca ccaccacttc ttcaaatcgt gtgccgtcgg
    30241 tgaactcaag ggtaatggca ttggcgatgg cgcgtttttc cgggtcgtgg tagtcagcgg
    30301 taaatgccgg atcttcaaag caattgatct tctcgcgcag ggcgtcgatg cgtttatctt
    30361 gcgcaacgtt gtcctcgtaa tctgccgccg ttaagcgccc gaataacagc gggatcgcca
    30421 ccatgtactg aatgcagtgg tcgcggtcag ccgggttatt gagcggtcct tttttgtcga
    30481 tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt ttcgatatcc gccgccgtct
    30541 tgcctgctgc ctgcatctgt tcatagagcg tcatcgccgc ttcaactgcc gtctgggagt
    30601 ggaactccgc cgggaaggag attttgaaca gcacattctc catgacgtaa gaaccgtacg
    30661 gacgctggaa gcggaacgat tcacctttaa aggagacgtc gtagaagccc cataccggcg
    30721 cggtcagggc tgacgggtaa cccatttcgc ccgttttcgc catcagtgcc agacgtaccg
    30781 cgcgggaagt ggcatcgccc gccgcccagg atttacgcgt gccggtgttc ggcgcatggc
    30841 gataggtgcg cagcgactga ccgtccaccc acgccagcga aacggcgttg agaatttcct
    30901 cgcgggtcag gccaagcatt tcggcgacca cggcggtgga agccactttc actaacagaa
    30961 cgtggtcgag gccaacgcgg ttaaaggagt tttccagcgc gatgcagccc tgaatttcat
    31021 gggctttgat cattccggtc agcacctgtt tcatggtcaa cggcgctttg ccgctggcga
    31081 ccgcgttgcg cgaaagccag tccgccgttg ccagaatgcc gccgaggttg tcggaaggat
    31141 gcccccactc cgccgccagc caggtatcgt tgaaatcgag ccaacggatc atcgcgccga
    31201 tgttaaatgc cgcctggacg gggtcgagct ggaactgagt tccggggacg cgcacgccgt
    31261 taggtacgac ggtgccgggg acaattggcc ccagcagttt tttacaggct gggtattcga
    31321 gagcttcaag accgcagccg agcgtgtcga gcaggcagta atgtgcggtg tcgtaggcta
    31381 ctttggagct gatttcgtag ttcatgacgt aatcgacgat atcaacgatt tcacgatcaa
    31441 attccgggcg gatgttgttg atttgagctg acattgggta cgtttcctta ttgttattcg
    31501 tagaggttta ctggcgctta tccagcgcga caaactggcg gtcttccggt ccaacataat
    31561 tggcggaagg acggataatt ttgttgtcct gacgttgttc gataatgtgc gccgcccagc
    31621 cggtgacgcg ggcgataaca aacagtggtg tgaacatctc ggtgggaacg cccatcatgt
    31681 tgtaggaaac agcggagaac cagtcgagat tggggaacat ctttttgctc tcccacatca
    31741 ccgtttccag gcgatcggcg atgttgtaca tcttcagcga gccgccttcc tgcgagagct
    31801 gcttcgccac acgtttaatc acctggtggc gcgggtcggc gatggtataa accggatgac
    31861 caaaaccaat gaccacttct ttgctttcca cccgcttgcg gatatcggct tcggcttcgt
    31921 ccggcgtttc gtagcgttgc tggatctcca gcgacacttc attcgccccg ccgtgcttcg
    31981 gcccgcgcag tgcgccaatc gcgccaataa tcgcggaata catatcagag ccagtgcccg
    32041 caatcacccg gctggtaaag gtggaggcgt taaactcgtg ttcggcgtac agcaccagcg
    32101 aaatatgcat cgccttttcc cagctttgcg acggcttttc gccgtgcagc agatgcagga
    32161 agtgaccacc gatggagtcg tcatcggttt ccggctggat gcgttcgccg ttgtggctgt
    32221 agtgatacca gtagagaaga atcgagctaa gtgacgccag cagtttgtcg gcaatatccc
    32281 gcgcgccaga gacggtatgc ccctcttttt ctggcagcgt gcagccgagc gcggaaacgc
    32341 cggtgcgcat aacatccatc gggtgtgacg ccgccggtaa ggcttccagc acggtacgca
    32401 cgttagccgg taaaccgcgc agggctttca gtttcgtttt gtaggcggcg agttcgtcac
    32461 gggtcggcag tttgccgtgg atcagcagat gcgccacttc ttcaaattcg caatgttccg
    32521 ccagatcaag aatatcgtag ccacggtaat gcaggtcatt gccactttta cccacggtgc
    32581 agagcgccgt attgcccgcc ggaacgccag aaagtgccac agattttttc ggtttaatga
    32641 catgggtact gttttgcagg atcgttgtgt cgctcatatt gtcctcgtca tttgtagggt
    32701 cgagatattt ttcgttgtgt tttatttcac ctggctacgg gcaaacaggt cgtcgagctt
    32761 ctcttcgtac tggtagtagt tgatgctttc gtacagctcg ttgcgggttt gcatggtatc
    32821 gatgacgctt ttctgcgtgc cttcctgacg caggacgttg tagacatgtt cagcggcgcg
    32881 gttcatggcg cggaacgctg aaagcgggta cagcgccatt gcgacatggg cgctgcgtaa
    32941 ttcgtcggtg gtaaacagcg gtgttgcgcc aaattcggta atgttggcga ggattggcac
    33001 ctgcaccgca tcggcaaact ggcggtacat ggcgagttcg gtaatcgcct ccgggaacaa
    33061 catctcggca cccgcttcaa cataggcctg cgcacgctcg atcgccgcat ccagcccctc
    33121 taccgccaga gcatcggtgc gcgccatgat cacaaaatca ggatcggttt tcgcatccac
    33181 cgccgcgcgg atccgatcca ccatctcttc tttcgagacg atcgctttat tcggacgatg
    33241 accgcagcgt ttcgcaccaa cctgatcttc aatatgcaat cctgccgcac cggctttaat
    33301 catcgatttc acggtgcgcg ccacgttaaa ggccgaagaa ccaaaaccga tatccgcatc
    33361 caccagcagc ggcagcgaac aaacgtcggt gatacggcga atgtcggtca gcacatcatc
    33421 aagggtagaa ataccgagat cgggcagccc cagcgaacct gccgccacgc caccgccaga
    33481 aagataaatt gcctgatatc cggcacgctg cgccaacagc gcatgattag cgttgatggt
    33541 gccaacaatc tgcaatggat tttctttagt cagtgcagcg cgaaacgctt tacctggaga
    33601 gtgtagagac atagcccatc ctttgttatc aacttgttat ttgcgttgat aaagacaaag
    33661 caaggggtgt gccacaattg ccttaataaa attaaatgtt taatttcaat ttgttagaga
    33721 taaagtcatg aactaatccg caaatatgcg tttcagttaa cgtttcaggc aatgtttcac
    33781 cgcgtttcat tgcaacaatt atgaaacaag actaaaccca atattcggtt tcttaacttt
    33841 gcggtgcgct atggcacatc caccacggct taatgacgac aaaccggtta tctggacggt
    33901 atctgtaacg cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg atcacctggc
    33961 gaacattacc cctattcagc ttggctttga aaaagcagtg acctacatcc gcaagaaact
    34021 ggcaaacgaa cgctgtgacg ccatcatcgc ggctggctct aacggcgcgt acctgaaaag
    34081 ccgcctgtca gtgccagtta ttttgattaa accgagcggc tacgatgtgt tacaggcact
    34141 ggcaaaagcc ggaaaactca cctcttctat cggcgttgtc acctatcagg aaaccattcc
    34201 agcgctggtg gcgtttcaaa aaacctttaa tttgcgcctc gatcaacgta gctacattac
    34261 cgaagaagac gcacgcgggc agattaacga gctaaaagct aacggcaccg aagcggtggt
    34321 cggcgcgggg ctgattaccg atctggcaga agaagccgga atgaccggaa tttttatcta
    34381 ttccgccgcc accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc gcatgtcgtt
    34441 acgccataac actcacgatg ccacccgcaa cgccctgaga actcgttacg tgctgggcga
    34501 tatgctcggt caatcaccac agatggaaca agtacggcag actattttgc tgtatgcccg
    34561 ctccagtgca gcggtgttga ttgaggggga aacggggacg ggcaaagagc tggcggccca
    34621 ggcgattcat cgggaatatt ttgcccgcca cgatgcgcga cagggcaaaa agtcgcatcc
    34681 gtttgttgca gtcaactgcg gggcgattgc cgaatcgctg ctggaagcag aactgtttgg
    34741 ctatgaggaa ggggcgttta ccggctcgcg acgcggcggt cgcgccgggc tgtttgaaat
    34801 cgcccacgga ggtacgctgt ttctcgatga gattggcgaa atgccgctgc cgttgcagac
    34861 ccggctgctg cgggtgctgg aagaaaaaga ggtcacccgc gtcggcgggc atcagcctgt
    34921 tccggtggat gtgcgggtca ttagcgccac tcactgcaat ctggaagaag atatgcggca
    34981 agggcagttt cgccgtgacc tgttttatcg gctgagtatt ttgcgtctgc aattgccacc
    35041 actgcgcgag cgggtggcgg atattctgcc actggcggaa agctttttga aagtgtctct
    35101 ggcggcgctc tccgccccgt tttctgccgc attacgccag ggattacagg caagcgaaac
    35161 cgtgctggtg cactacgact ggccgggcaa tattcgtgaa ctgcgcaata tgatggagcg
    35221 actggcgcta tttttaagtg tggaaccgac gccggattta acgccgcaat ttttgcagct
    35281 gctactgccg gaactggcgc gcgagtcggc gaaaactccc gctccacgct tactgacacc
    35341 acaacaggca ctggagaaat ttaatggcga taaaacagca gcggcgaatt atttaggcat
    35401 cagccggacg acgttctggc ggcggctgaa aagctgaact gccgaatgca gcgcgaacgc
    35461 tttatccggc ataaatttca atagatgatc accgataaac ttacgcatcg ggcatgttta
    35521 catagggttc agaattactt cttcttataa atatttgccg tgccgtggat cttattgtca
    35581 gtttgaccgg aggtcagtac caacacatca gccccttttt catccgcttt tttgatcaaa
    35641 tcttctttcg cgtctgcagt cgacatttca ttgctggttg aaatatcacc tattttttca
    35701 tactgcgatt caactttttc aaattccgct ttggtcatca attcagcggc atagacattg
    35761 gtaacggcta acgctaacgc accgactaac attttagaga ttattttcat attacggctc
    35821 cagagttgtt atcacactat cacccaccca cattattggg ttgattaata attagttcag
    35881 tcagggcata aacgccagta accggaaata agacactttg tgatctctct cgcacccgat
    35941 tatttttggt agaaatatga tgaagttact tcacttattt atggatgaaa tcactatgga
    36001 tcctttgcac gccgtttacc tgaccgtagg actgttcgtg attacttttt ttaatccggg
    36061 agccaatctc tttgtggtag tacaaaccag cctggcttcc ggtcgacgcg caggggtgct
    36121 gaccgggctg ggcgtggcgc tgggcgatgc attttattcc gggttgggtt tgtttggtct
    36181 tgcaacgcta attacgcagt gtgaggagat tttttcgctt atcagaatcg tcggcggcgc
    36241 ttatctctta tggtttgcgt ggtgcagcat gcgccgccag tcaacaccgc aaatgagcac
    36301 actacaacaa ccgattagcg ccccctggta tgtctttttt cgccgtggat taattaccga
    36361 tctctctaac ccacaaaccg ttttattttt tattagtatt ttctcagtaa cattaaatgc
    36421 cgaaacacca acatgggcac gtttaatggc ctgggcgggg attgtgctcg catcagttat
    36481 ctggcgagtc tttcttagtc aggcgttttc tttgcccgct gtgcgtcgtg cttatgggcg
    36541 tatgcaacgc gttgccagtc gggttattgg tgcaattatt ggtgtattcg cgctacgcct
    36601 gatttacgaa ggggtgacgc agcggtgagc agataattta ctctctacgt aatcaacctg
    36661 atttgccgaa tcccggccat cggcgatgat agcccccgta atgcgtggtc cttatttccg
    36721 ataagtttac ttgagtcttt agcttcccat ttcagcccac tctcccctgt gtgggctttt
    36781 ttcattggtc tgacccgtcc tgaatagcca aacaatggca atgttccact ttgcagggat
    36841 gataataagg ggatctgatt cagaagagtt ttgaaaagtt ggaccgccat cgaggcctcg
    36901 aaccccgtac catacaacaa tcctggttgt cgtctgctct tcctgatgag ctaatggcgg
    36961 tttttgatat gagccttaca gcatattttc tgtcaggctt gagttcgatg ctaaggtaca
    37021 gaaaagaccc cacacaaaca tgatggaatt atgatttttt tcatatgaat gtcaacccac
    37081 acattcacaa aacatctgca tgagatcatt ttttatacac cgcatagctg cgaaaccact
    37141 gctggtgctt ttttaagcga agcgtcgccg atcacaacaa aatatcggcg aaaaaggcgg
    37201 tggaatgcca tttggcatac ggatatacag atgaaatgat tacctgcttt gcgattcact
    37261 ctctggtgga ataacaactt gcaggtggaa ccctttctga actaaagagt gtagcggatt
    37321 ttaacgtgct tatttacagg ataatatctg tcacctaatt cctgcactct attcgatatc
    37381 cattcattta atcgtgcatc atctctgtat gtttttacgt ccagaacaga gagcaataac
    37441 atcaccagtg attcaggaaa aacagggtag atatttttta agtgtaataa taatccatgc
    37501 gcaagcgcct gcttaatggg cgcgtctgtc tccggaatag acgacatttc gcgaagccat
    37561 ttttccacac gcatacactc tttcccttta gggaaaattc tcagcaaagg catatggtgt
    37621 tcaagaagaa tacatttgca atctgttcgc actgaatcga taaactgcac taatttctta
    37681 agcccggtaa agcgtccagc ctgcacattt gctttaatat caagaaacgc atcatcctgg
    37741 tcatataccc aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg
    37801 tgatcagggt aatccatcca gtcataaaat cctgggaaat cattaaactg cacatttatg
    37861 gtgagtgcgt tatcgtctga ccactcacag tctcgtacgg cagccatgag atgaatgagt
    37921 ttgtcgtcat cgactaaatc atcacgacaa tcataaggat aatcataata ttgtttaatc
    37981 aaaaccttga atatatcgac acaggaaacc agggagcttt tgatatcctc tggtaactga
    38041 ttgtttttaa ccgtattaag tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga
    38101 gcttttaatg atatcatcgc gatctctctt aaataaaaat aaacgtcgca gataataaag
    38161 cgctgattaa accaggcact atcagaaatc gctcataact aacgtgtgaa gtattgtgta
    38221 ctggagggag ttaatttaac aatttgaatt tacgttatag gttttactgc tactttgata
    38281 ttatttcgct ctccttaact aaaaagttaa gtggagagcg aagttaatat gttaaaccac
    38341 agcgtattta ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt
    38401 tcacatcacc acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct
    38461 ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa
    38521 tgccgccaat catagaaccg gctatcgcac ggcgtttcat gatcaggttg aaaacttccg
    38581 gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca
    38641 aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt attcaaaatg aaatcgaaac
    38701 tcttcagatg agccgccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca
    38761 gggcttttgc cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca tgcgccccca
    38821 tcgcgtgggc cagcttgatc cccatatgtc ccagaccgcc gatgccgacc acgcccactt
    38881 ttttacccgg cccggcctgc cagtgacgta gcggcgaata cgtggtgatc cctgcacaca
    38941 acaaaggagc caccgccgcc agctgctctt gcgggtgacg aatacgcaga acatatcgct
    39001 catgaacgac gatctgttgt gagtagccgc ccagagtatg gcccggttcg tccggcgtcg
    39061 gcgagttata ggtgccggtc atgtgatcac agtagttttc cagcccgtct tcacactctt
    39121 cgcaatgttt acaactgtcg acaatgcagc cgacaccgac cagatcgccc ggcgcatgtt
    39181 tttctacctg atcaccaacg gctaccacac gccccacaat ttcatgaccc ggtacgcagg
    39241 ggtaaaccgt ccccgcccac tcggaacgga cctggtggag atcggaatgg cagacgccac
    39301 agtaagcgat ttcgattttg acatcattcg gtcccggttc acgccgtgtg atatccatcg
    39361 gttcaagtgg ttgtttagcg gaatatgcac caacagcttt gatcttcatt gtgtttactc
    39421 ctgattagct atgtgtattt ggcgacgcct gatatgtcat cgcggcaaaa cgcgtccatt
    39481 gaatatagcc aatatttaaa ccacccgatc cagccccaac atgccgcgaa cctgaatgaa
    39541 acagagcagc aacgttcccc ataaggccat cgtcaggtag gggcttatac cgagaatatt
    39601 gaagccgctt tcaataattt gcagaagaaa cagcgccagc accataccaa ttatccgccc
    39661 gctgccgccg tccggattaa ccccaccgag tactgccgcg aggattgaca ccagcaagta
    39721 cgattcgcca taagaggcct tagccgagtt gagtttcgac atcatcaaaa atgccgctac
    39781 cgcacaaagc aaggcggaaa tgacataaac ccaaatcagc acccgacgag tgttaatgcc
    39841 ggagtaaagt gttgctcgtt cgttagaccc aatcaggtaa atggctttac cgagcggcgt
    39901 tttctccagc aatatccata gccccagtgc cactgcggca aacaaccaca tcggtagcgg
    39961 aataccgaac cattgcgcat gattcaacca cagcacccat gagggataat tggcaatggc
    40021 gctaccacct gtcaccagaa tatttatccc tttgagcaac gtcatcatcc caagcgttgc
    40081 aagaattggc gacactcgaa taccagcaat caatacgccg ttacataaac cgattatcac
    40141 cgccgcgcca gcgcccgcca gtaacgtagc gacaaccgta gcaatacccg gtggatactg
    40201 agtcgccacc cacgccatca ccagtgaaca ggcgttagca gtggctataa ttgacaagtt
    40261 aatgccaccg caaagcatgg tcaccgccat agccaacgcc agaatgccca gcacgggcat
    40321 ttgcgaagcg acggactgga aattacttat cgaccagaaa atctccggca tagtaatgct
    40381 gaacgcggca acagttagca ccagcaggcc gatcaagtaa aactcaacgt tattacgcca
    40441 cgattttttc atagcagact ccggttctga tgctgactcc acgccgtggc actaatgctg
    40501 gcaacgatga tgatgccggt gatcaatgtt tgccagtaag acgagactcc caataaattt
    40561 agcccgtttt gcatcactgc caacaacacc acgccgagca aagtaccggt taacgtgccg
    40621 cgcccgccga gcaaactagt gccgccaagc accaccgcag ccagtactgt cagctcataa
    40681 cccagaagag aatcgggggc gacagtcatc accgtccacg actgcactac acccgcagcg
    40741 ccagacatca atcccatata gccatagacg aaaagttgca gtttcagcac gctaaagccg
    40801 atgcgactgg ctgattcgcg gttcccgcca agggcataaa ttttgcgccc tacactggtg
    40861 aaattcatga taaacgctgt tagcagcacc accgtaatca tggcgatcag cggcagacca
    40921 aggccatagt catagccatc agcatcggtg tacttgaaca acataacccc ctgctcaaac
    40981 cacggcggaa agtcgtacag ccacacacct ttactcaacc ataacagcag gccatagaaa
    41041 atattcagcg tcgagatagt gatgatgata gaaggcaccc gtaggcgatt aaccagtaag
    41101 gcgttaatta agccaagtaa aatgccgatg cagcccgcca gtgcaaacgc gacagcgaag
    41161 ttgccaccaa ttttttgcag caacagcacc atgccgtatt gcgcaatgat ggtcattgct
    41221 ggaaacgaaa tatcaattcc accggaaatc aacaccacaa acaaaccaca ggcgagaata
    41281 gtcaacatgg catagttatt ggcgagatcg tacaaattac cgaatgtcag aaactcgtcg
    41341 ctgcgccagg caaggccaac aaacagcacc acaatcagta atgccagcca gaattcatgg
    41401 cgttttttca gttcagccat ttaccacctc cgcaatcctg gcctgagaac tgctgtcagg
    41461 caggaagcta tgggtgatct gacctttttg cattaccaaa atccggtggc tttgatacca
    41521 cgcttcttca atttcatcgc agatcatcaa taccgcaatg ccatgagccg ccagatcgct
    41581 gataatgccg tatattcctg ctttgttggc gatatcaaca ccgactgtcg ggctatcgag
    41641 aatcaacagt cgtggcccaa tcgccagcca tttggcgatc gatactcgct gcgcgttacc
    41701 accagaaagc gtattcaccg gaagatgagg atcagagact ttgatggtca atgccttgat
    41761 tagcctgtcc accaggtcac gaactttagt ttcactcaga aagccgaagc gatcttttac
    41821 cttgtgaaaa acagtgctaa tgatgttgtc ttcaatagat tgcgccatca ccaaaccgcg
    41881 cgacatgcga tcttccgaaa cataaccaat accgtggcgg atagcgtcct ggttactatg
    41941 cagtgtaacc ggctggccgt tgatgaggat ctcgccagca tccggtcggg tcataccaaa
    42001 cagactcaga cataattccg tgcgccccgc accaagcagg ccaacaatcg ataccacttc
    42061 gcccgcttcc acccgcaagt taatatttag atattcccca tgacgactta aattacgcac
    42121 ctcaagcacc ggggtgctgg attttccctg ccacaactct cgcacctgat attcgaactc
    42181 ctggccggtc atcaggaaac cgagttgttt tgtggtcatc tctgcagccg gaaaagtccc
    42241 gaccagctca ccatctttca gcactgaaat acggtcggaa acttccatca cttcttccag
    42301 acgatgactg acgaagacca cgcagatccc gcgttcccgc aattgatgca ccacctgtaa
    42361 taacccttgc acttcctgat gggtcagcga agcggttggt tcatccatca cgatcaaccg
    42421 tgcgtcctgc gccagtgcac ggcaaattgc taccagctga cagcgggcaa ttgaaagctc
    42481 agaaacgagt gtatccagtg gcaatgtgac gttaatactg gtcattgccg cctgcgccac
    42541 ctcacgcaga cgacggcggt taacaaacag accgtggtga tagtgattca cggcgatatt
    42601 ctcccagaca ctcaggttag gaaataaaga gaggtcctga taaatcacct gaatcccctg
    42661 cgccaccgaa gcggcgggtg tcagctttga ccagtttttc ccaccaagcg taatttccgc
    42721 tccctcatcc gggcgataaa caccggacat aattttaatc aacgtcgatt taccacagcc
    42781 gttttgcccg gcaaggcagt gcacttcacc cgacataaag tcgagagaaa catcgcgcag
    42841 cgctcgcgtg gcatgaaacg ttttgttgat atgacgaagg gaaaggaaag tttccataac
    42901 atcccctggc aataagaatc ggggctttcg ccccgaagaa atggattaat aaagtgagtc
    42961 gatattgtct ttgttgacca gtaatacctt atggaaacgg ataattcgct tatccatatc
    43021 tacgtcggcc ttacccagat tctgcatttc taatccaggt ttgatctctt cacccttcag
    43081 caacgtgctg gcgacggctg cgagtgcgta acctgcagag gccggatcgt aagtaatccc
    43141 ttcggtgata tcaccacttt taatcagtga tgccgcctgt gaagggatca tcatgccata
    43201 gactgcgact ttatttttcg cccgtttctc tttcaccgca cgtcccgcgc caatcggacc
    43261 gtttgaacca aaggagacaa ccgctttcaa gtcaggatag gttttcatca ggtccagtgt
    43321 ggtacgacgt gagtcatcca cactctcggc aaccggcatg cggcgggtaa cttcatgcat
    43381 atccgggtaa tgctctttct ggtatttcac cagcaagtca gcccataagt tatgctgcgg
    43441 cacggtcaaa ctacccacgt aaatcacata gccgcccttg ccccccatgc gtttcgccat
    43501 atgctcaaca tattcagcgg caaatttttc gttatcaatg atttcaatat cccagttagc
    43561 acttggctga ccgggggatt cgttggtcag aaccacaatt ccggcatctc gcgctttttt
    43621 gaataccggt tccagcacgt tggcatcgtt tggcacgata gtaattgcat taaccttacg
    43681 ggcgattaaa tcctcaataa ttttaacttg ttgcggagca tcagtacttg aaggccccac
    43741 ctgtgaggca ttaacaccaa aggctttacc cgcctcaacc acaccttcgc ccatgcgatt
    43801 aaaccacggc ataccatcga ctttagaaat attcaccacg actttttccg ctgcctggag
    43861 cggcgcagaa attagcgcag cgcctaataa cagcgaagac accatattga taacaaaacg
    43921 tttattcatc atttcagcct cattgcaggg tagtcaggaa gtgttcgccc aaattagcgc
    43981 gctggagaga ataagaattt tcgaatattt ttatgcagaa taaacagccc tatttcaggc
    44041 agctattaaa aatatggtta taaatatgga caaaacgggc agcaacaata agtgtgacat
    44101 tcatcacgta aaaaatagca aaataaacaa aattagcgat attttatgtg tgtgagttta
    44161 tttcgcgtag ataaatagtt atctacacaa cgtcaataag ggcatattct tcaaatataa
    44221 cgtccatttc tggtgaatag cttccttctg cctggaaaaa acgacggtgt tcattacgcc
    44281 aatattcaag actgaggtca ccttcacctt ccttacgggc aagctccacg gtgacatcag
    44341 aaaagcgcag taaatgcagg ccaatcactc tgataacaca tacaggctca ccacggctat
    44401 tttctacaat cttatactca ccaatcatcg gaaatgcgtt gtcttcaata catcccgcca
    44461 gcgaaccaca ggcagctgtt ttaacaccag tcgcaatcaa cgttgcgagg tgatcggcca
    44521 acgctgctga atcgccaaaa gtacaaaagg aagcatgggg atatttttgt gagaattttt
    44581 cttttatggt catggcaata tttttgcgat tacggaaaaa caaatcacat cttaattact
    44641 gttggtaacc atcagcaaga gtatcattgg tttttcgata ctgaatcttt tttatccgaa
    44701 gcaggtcaca tttatggcct gataaatacc tttgttaaaa ctcacttttg tgtatttctc
    44761 tcaaataatc ccctggaaat tatgccgcga gattataaag ttcaggatca ttcattagca
    44821 aaccattccc gccccactcc ctggggctta aatctcttac aattcaccgt tgcaggcgtt
    44881 tcaaatgcgc atcaacctgc cacactcccc cacaccagtt gccctttatg gaacgttgcg
    44941 gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggcgtccac cagcacaaag
    45001 ctggcatcat cctgcgcttt tggccataca cgctcgcctt tttcattcag cggcaataca
    45061 tcgccagtgg ctagaaacag cgaacgggag aggttctgtt cgttaggacg aatatagagc
    45121 tgcgcgtaga gattggcttt ttccagcatg tcgcccagac cataaggcga ccagtggtcg
    45181 ataacgctgt cagtgccagt catcactttt acgcctttgt cgtgcaactg tttgagcggc
    45241 atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc cgccatccga
    45301 ttcgccagcg catcgacctg ttgttcgttg agcgtagcca gcgcaaaggc gtggctgatg
    45361 gtcagcttac ctttcagttg cggcgttttc tctaccgttt caaccatata attgatggct
    45421 gccacgcccg acggagtggt ttcgtgcagg tgaatatcga cacctttgtc gtagtccagc
    45481 gcaatctgaa acatggtgtc gagggatttt tccatcgcgc catcgacact ggtcgggtcc
    45541 agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc gcattaaggg ttccgatttc
    45601 gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc cgcctgacgt
    45661 cgcgccagca ccgcctgcaa attctgcaga tttttcaggc cggaaaccgg ttcgatattg
    45721 cagtggctgc gggcaatggt ggtgcctttc gactgcaata gatcgatcag tttttctgcc
    45781 cgttcctgcg tgtacggttg cagttccggc agcattttct gctcaagctt gatcatgtcc
    45841 tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta gaaggttttg
    45901 tcgagatgaa tatgcatgtc gcgagtggtg ggtagcatca acttaccgcc agcgtcatag
    45961 tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcgacaat tttgccgtcc
    46021 tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg ccacaccgtt ttcgtagtca
    46081 aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat tttcatggtg
    46141 ttgttcgctt cgcagtttag ggtgccagct accgccgcat gggcgagcgg cacagaggta
    46201 ccaaacagcg cggcggcggt gaccatctta ccgctctggc tcagaaattc acggcggcta
    46261 ttgctttctt tcatcctacg tccttacgtt ttaataatat gggtgcccgt ttcaccgcgc
    46321 agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ctgctccagg
    46381 aacgtcaggc tggcgatgat ttttggcaac atgctgccgg gcggaaaatg cccttcctgc
    46441 atatagcgag tcatggtggc aatatccacc cgatccagcg cctgctgctg cggtttacca
    46501 aagtggatac agactttttc gacgccggtg gtgatcacca gaatgtcggc gtgaatttca
    46561 cgggccagca gcgcggtaga gagatctttg tcaataaccg cgtccacgct ttggtaatcc
    46621 cccgcttcag tacgcactac cggaattcca ccgccgcccg cgccaattac gacaaaacct
    46681 tgttggatca gcgccttaat ggcgggtgct tcgacaatac gtttcggttc cggcgaggcg
    46741 accacgcggc gatagccccg tccggcatct tcaacaaaac accagtcagg gtttgccttt
    46801 tgtagtttgt cacgctggct ttcactgaag aatgcaccaa tgggcttggt gggatgggca
    46861 aaacctggat cgtttttatc cacttccacc tgagttacca cggtgacggc ttttttctcg
    46921 ccgtgacgcg ccagccggtt attcagcgcc tgttggatca gatagccgat gccgccctgc
    46981 gtatctgcca cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc gtgggcaatc
    47041 tccgcacggc gtaaatccag cccgacctgc ggcccgttac cgtgggtcag cacaatgtcg
    47101 taatcggaag ccagcatttc cagcaccgta tcggccacgg ctttcaccgc ctccgcctga
    47161 tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat ggcaacgacc
    47221 acaagctctt tcatgttgtt atgtccgcta cagagggtga ttaggttatg accaacgcca
    47281 gactgcctga tgcgctacgc ttatcaggcc tacaattctc gggccatccg taggccggat
    47341 aaagcgttca caccgcatcc ggcataaaca tgtaagtcag acgccgtaac gttcgcacca
    47401 gccgagaatg gctttctcga agcaggcgaa tggtggatgc acaatgcccg cgccaatcat
    47461 gccgacgccc gcatctttat gggcgatggc ggtgttgatc accggcagaa tgccgctgct
    47521 acccacgcgg gtgatgtcga ttgccgacgg cacgcccata aaaccgagca gcggaatggt
    47581 gacgttgggg ttttcgccaa gggtgatttc gcgcatctga cgggagaaat cgatagcctc
    47641 ttccaccgtg ccgcccacca gtgcgacgat tgcaggcgcg gtcgccatgg caaacccgcc
    47701 aataccgtag gtttctgtga tggcgctgtc gccgatatcc agccccgaat cttccggctt
    47761 ataaccagca aacatcgggc cgatgacctg ctgcgctggg ccggtaaacc actgccccgg
    47821 caggccgctg acccgcaggc cgaactcgac gccgttacgc gccatggtgg tgaccacggt
    47881 gctgtattcg atgccgtgcg ccgcatccat cgccgcttta cacatcgcca tccacgtcgg
    47941 gccggagaag tagtcgctgc tggcgacaaa ctcgaacact tcgcgctgtt gctcgacgga
    48001 atagcctgcc tgaatgatcc ccggcgtcag tgcctgaatc agcagcgtcg tcccggcgtt
    48061 attgcggtta tggcactcat cgcccatatg cagcgcctgc gccagcatta agcgcagatc
    48121 gatctcaccg ataattttca tcgcgtcgcg cagcattggc ccctgcacat cgcgcatcca
    48181 gttgaggcgg tcgatcacgc tctgatcgtt ggcacccata cgcaaaatct tcgccatctg
    48241 ctcgctcata ttggtataag cgatgttgcc gtaggttttg tttttcacga tgtgcataaa
    48301 catcgaggcc gaggtaacgc ccgccatcga tcccacgcag tcgtgttcgt ggcacggcga
    48361 gaaggtgatc tccccggagg ccgccagttc agtcgcctca tcgagatctt tcgccagccc
    48421 ttcgaacacc aacgccccgg tgaccgcgcc tttcatcgcg ccgcacattt tttcccaggt
    48481 gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg cccggcacca cgttaatcgc
    48541 ctgatcaaaa ccaatcagca ccggatgcga ctggataatg cgctccagcg cctgctggtt
    48601 agcggcggcg attttgtctg ccagcggcga atcggcaatg ttatccagcg cctgcaccac
    48661 ctgcatattg ccctgccccg gcggcgtcca gtcgagttga gtcacttcta catgctgctt
    48721 tttcaggtca tcgctaaaca tggcgatacc gacgttaata acgttcaacg gttggctaaa
    48781 cagtgactgg ctcatcaggc ttcctcccct ttgcagataa attcacgcgc cagcaatccg
    48841 gtattggtgc tgctgctcgc cagaatcact ccggcatcaa gcagcatctg gctttgttgt
    48901 tctaatgatg gcgtatcgag atcggtgccc agcacatagg cgagaatgat caactcgcgt
    48961 ccttcggcgg cggcgatcgc tttcgcttct ttgatcgcct cgatggtgga gccgaccgga
    49021 tcttcatgcg atccaaatcc gagcacaaaa tccatcacga tcaccgccac ttctggatcg
    49081 cgcgcttctt cgatcaagcg actgatgcgg ttggtggggt caatcattgg atgcggcttg
    49141 ccgttggtga agtcgtcatc gccaaagtcg aggaaggtgt gtttgatgct gcggttgata
    49201 tcttgcaggc ggaattcagg atcgggctga atgttgctgt agacatcgcc atgtttttcc
    49261 atcaccgcga acatggtttc gtcgcacagc gtgccgccgc agaacaggcc acgaatgtat
    49321 ttctgctgcg gttgcagacg cgcacgcaca tcagcaatca acggctggtt aagcgtatgc
    49381 aggtcgagat tttcctgttt cacgccggag agcatcaccg cttttagcgc cgcctctttg
    49441 ctgccgcggg caaactgtag cccctgctca tccaccggcg tttcaacgcg accgaggaag
    49501 cagaccacca ccggcttgcg gcaggcacgt gcgcgttcca gcactttgcg ggccaccgca
    49561 ggcgcgggag gttttgaaat cagtgcgata atttcagttt gcggatcgtt ttccagcatc
    49621 ccgatggcgt cgagcatcat caggccgccg attttctcgc tcaggtcgcg cccgccggtg
    49681 ccaatcagtt gtgaaacacc gccaccaaat tcatgaatgc ggacgctcag ctcctggctg
    49741 ccggtgccgg atgcgccaac aataccgata ttgccgcgac gcacggcgtt accaaaacag
    49801 agcgccgcgc cgttaataat cgccgtgccg cagtccggcc ccatcatcag cagccctttt
    49861 tcgtgggcca actgcttgag cgccagttca tcttcaactg agacgttatc ggaaaacagc
    49921 atcacgttaa ggtcgttttg cagcgcctga cgcgcttcgc gagcggcaaa cagaccgtta
    49981 accgaaatca ccgccaggtt actttccggg atatgctttt tggcgctggc aatagtggcg
    50041 taacgcgcct cgtgcgagcc gctttgcgct ttgctgttga acagttcttc aatctccacc
    50101 agcaactgtt cgttgtccgc gcccgctttg ccgttgatga caatcatcag gtcgccgttt
    50161 ttcgcctgtt ccagctccgg cgtcagcagt cctaaatttt tcagtacacc tttgttcatt
    50221 tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga gtttatttgc acgcgtggag
    50281 atagacatca gcgagacaga atcaaaatag gtattcggtt taatgactat tttaactgac
    50341 ataattccct ccgtaagttt ggctcagcgc acaaccatcg gccatgccat acagcatgtc
    50401 gcagccggaa ctggagccaa tatgtttaat tttttctatt gcctgagttg cgttcccagg
    50461 gatgtcataa ataatgttgt gaataaaacg atgaatattt tcccggcaac gttgttgtaa
    50521 tgcggcttcc agcgttatag cacttaataa tgtggtgtta tttctgccgc gctgcatacc
    50581 gagataaaat tcctctttgt atttttccga cggatgcccg ggaataaata aaataagcgc
    50641 cagacctgtt aaataatcgt cggctgatgg cgtaaggcca atccctaacc caatcatact
    50701 ggcgaccgct gcggcgatat tttgtttttc gtttactgcc tgaataactg cccgtcgttt
    50761 aatatgtagt tctttattta acgcctgata aaacggatta tcgccgcgat aattaaacag
    50821 ggtttcattt tcccccaggt gctgatgaat aatgtcgtgc cactgtagcc aggcgataag
    50881 gtgaaaattc acagcggtca tttgcaaggt tggggacagc caacgccgac aacgactcat
    50941 ctctatatga agatgttgac caaccgtaat accttgatcg tcaaactgaa ccttatcacc
    51001 atgccggaac agatcatcaa agtgagtgag tgccaaccga caactgtttg gcgcattgtc
    51061 gtactcttca caaagcagcg tcagcaactg ctggcgagcc gggagtgcga tatttaccgc
    51121 tctggcaaag acctgttcaa cctgtccttg cccccgctgc gccagaaaat ccgcatccgc
    51181 agttagcgcc cacacacacc gtctttctgg tgtgttgata gcaagcctcc cggtttatgc
    51241 acctgcggca atcagtaact gcgcaatctc ttcaaagccc cgttcccgcg ccagttccag
    51301 tggcgttttg ccatatttat cggtcagatg cgggctggca ccgtgttcca gcaataactg
    51361 cacaatcgcc tgctgtttaa taccaccatc attaagcaca atcgcttcca gtagcggcgt
    51421 ccagcctaca tggttggtct ggttaacgtt aatctccgta tgcgccaaaa gctcttttac
    51481 aatacttaaa tggccttttt cacaggcagg cgtcaggccg acaccgccaa aacgggttac
    51541 gcaattaaga tcgggtttag ccggtaaaat aattcgtagt agagttaaat catcgttcag
    51601 acagctaatt aaaaaaggat ttaaacaggt atgatcttgc ttattaatat ccgctccggc
    51661 atcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg taattgccgt
    51721 tttcccctga cgatcgcagg tattaatatc gacacccagc gcaaggcagg tttttacttt
    51781 atcgatatca ccctgttgtg cagccaataa atagtcggca ggtagatttt ttatactcat
    51841 tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa cgataatctg
    51901 ataaaagaac ccgcttaaat ttaattcgct gtatttcgcc tttttgttga atgatgttca
    51961 acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga taacctttca
    52021 ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttc gtccatgtct
    52081 aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc ggtatttgtg
    52141 cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg gaattattag
    52201 gaaccattgg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcggt aaatcggggt
    52261 ttatcagaag tctggcggtt aattattccg ggatggtgtg ggcatttttc gccgcgctgg
    52321 ctgccggttg gctggcctct gtaagtggac tgtccgcgtt ctgggcgagc gtaattacta
    52381 ccgtaccctt ctcggcggta gtggtctggc aagggcgttt ctggttactg tcctttattc
    52441 ccggtggctt tctcggcatg acgctgtttt ttgccagcgg gatgaactgg acggtgacgt
    52501 tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggt ggacaaaaat
    52561 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
    52621 ctttaaccga tacctctaca gtcatagctg gagaaattca tagtagataa tggcgagaat
    52681 ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac cgctgcgcgt
    52741 tttggcagct tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc cattagctac
    52801 accatcaagc gtatggagac ggggctggat gtggtgctgt tcactcgcag tacccgcagc
    52861 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct gctgaatgat
    52921 tttcacgcca tcaaacgcag tatcgatact atttcgcagg gcatagaagc acgtgtgcgc
    52981 atttgtatta atcagctgct ttatacgccc aaacacaccg cacgcttgtt acaggtgctg
    53041 aaaaagcagt ttcctacctg ccagattacc gtcaccaccg aagtgtataa cggcgtctgg
    53101 gatgcgatta ttaataatca ggccaatatc gccatcggcg cacccgatac actactggac
    53161 ggcggcggta ttgattacac tgaaattggc gcgattcgct gggcttttgc tatcgccccg
    53221 gatcacccgt tagcctttgt gccggaaccc atcgccgaaa gccaactgcg tctctaccct
    53281 aatattatgg tggaggacac cgcgcatacg attaataaaa aagtgggctg gttgctgcac
    53341 gggcaggagt caattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
    53401 gaaggaattg gttttttgcc ggattacatg gtccgtgagg cgatgacgca atccctgctg
    53461 gttacccgac aaatccataa tccgcgccag gattcgcgca tgttactggc gactcagcat
    53521 tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat ttgcgccaaa tggcatatta
    53581 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
    53641 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
    53701 ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc tttaccgatc
    53761 attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag accaggctta
    53821 cgcgctagtt cgacaatatt gtccacaata tgaccggaga tttcgtcgac actcgccatt
    53881 tgcacaaatg acttatcgat cttaataaaa tcgaccggga acgcctgcaa gtaacgatag
    53941 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
    54001 tcaaatatcg ctctggcttc tggcgttacc ggaataggat tacgttccgt tagctcgaga
    54061 accagcttga ttttatcgtt acctaatttc ttaaccaggt tcagacactc tttttcaaat
    54121 cccgctgcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt gtccggcagc
    54181 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcagttggcg ggtcattatg
    54241 acaataagac cggatgactc cgccagagga ataaactgat ccggtgggat aattcccgtt
    54301 tgtggatgtt cccagcggac aagtacctca cagcccgtca gtaccccagt ctgcgcgcag
    54361 aaaaccggtt ggatccacgg tttgaattcg tggttttcca acgccagact gatggcttca
    54421 ggcgtgacga tatggtgaca acggttatca gggatataaa aattattttt atttcccgtg
    54481 ccactgatga cttgcggatg atattgaaaa aagatatccc gccagaaggt aatatcactc
    54541 tggatgccta gtttttcata cagctgcttc ttttgatgtg aaattgtctt tgcactgcga
    54601 tttctgtact gtgagatttc tgtaaccgac attccctcca ttagcagctt gagtaaaagc
    54661 cgttcttttt cggacacgat accaggcaac gtcaaattca ccgttgcccc gaactcttcc
    54721 aaaaaaacac tcaaatcatg cgaagccatt gaacacacct tgattaaatc ttgtgcgcca
    54781 gctaaatctg cgagtcttca ctctgactga taaagctggc tgcaagaaaa tgacacagag
    54841 agccgaatta tctcccgttc ggctctctct ccttttactc attattcaga tcagaattgc
    54901 cacttaatgc caaccattgc agaggtgtca ttatatcccc tatcggcaac ctgaacgccc
    54961 acattccccc agacattcag gttggcattt agctgtcctt ccaccccggt ttttatctca
    55021 gcaatatttc gggctccatc ctgagtgacg gacacgccat ccatactggt gctgaaatcc
    55081 ttactgttat gcagccagtt cacttctaca aacggctgga actcgcggga tttaccgtca
    55141 tccattttat ggtggctctt cagccaggtt tttacgccaa gtcgggtttg aacattgcca
    55201 tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt gcttatcggc tttgactccc
    55261 atccaggtaa cctgtgcttg cggctgaaca taccattcat tacgcgttcc ctggctgcca
    55321 ttaaattcag ctaatttgtg tttgtatcca gcttccagtg aagcggtaaa tccttttgat
    55381 ttataggatt cactttgtaa gtcatcccct ttcactgtgt tatcaaacca gctgtactgc
    55441 gcccaactgt cgagatacgc gccattacgc gactcgtcat cggcatacca ggtggcatag
    55501 aggccggtgc tatatccgtt cacactcgct tttgcacgat aaccggttcg cgaggaaatg
    55561 gttttactgt cgctgttgcc atatcccgcc atgaccccaa catgccagct gtcgctgccg
    55621 ttttggctcc actgcgcgac atcgcctccc agttgcagaa catagcgatt gctttgggtt
    55681 ttcagctggc cgctgccatc acgccattta ttatgaccac cttcatggcg catccacata
    55741 gaggtttgtt tctgctcacc cgtcaccatg tcggtatagt acgtattacc cagacgctca
    55801 tgcagacgcg tggtgaacat tgtattcgct gctgcaaggt tcgcaatgta gctacccgcc
    55861 tccggtcgca ggtcattatc cacattcaga tccggacccg gcgtaggtgt cgggttaggg
    55921 ttcggctctg gattcgggtt tggctccgga ttcggcatcg ggtctggctc cggctgcagt
    55981 tcaggagaat cactgccgct ggtcagatac cagttaccac tatttgcccc ttgtccacgc
    56041 gcgagagtgt agtcatacgc ccccgcaacg atacgaccag cctgaacaaa ttcgccctca
    56101 gacttaccgt ctacatggat aagttcgata ccgttaaggg ttttcgcacc tgtaccgcca
    56161 gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc cctcgacaac caatttatcg
    56221 gttactgagt catcgctatt cagtaccgta ttcattacta gttgaccgtt attaccgtgg
    56281 tagttcccct tcaccactaa cacattgcca gcttgctgtc ctgacattcc ggtcatcagc
    56341 gtaccgttgt tggtcagatt gccgcccagg gtgtaattca cagacgcagc cgctcttttt
    56401 tcagcgctat tattcaacgc cagggtaccg ttgttctcaa cgtttgcgct acccaatccc
    56461 tgcgcatgac ttaccaggac attagccgaa ttagcaatgt tcacattcgc cgtcaggttg
    56521 ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat tcagctctcc gctccccgtt
    56581 aacgaaccgg ttgaagtgcc cccgttggta accgtcagac tgccgccatt taagctcagc
    56641 agtgaatcgg cgctgccatt gagcgtgccc acggtctggc tgtgaccatt catatccagt
    56701 tcagtctctg ccgccagacg cagttcatgg gtattaccca acacgttatc gttattcaac
    56761 aaaagcgtcc cactgcgcag atcggtaaca ccggtatagt cgttgtcttt gttagaaagc
    56821 gatacggtct gacccttctg gctgctgaat gccaggtcac cactgccggt aatctttgcg
    56881 ctgagatcgg cggcattctc gcttttaccg ttagagctca gcaccagagc atcgctgtcg
    56941 gtagcgtgta aatcaagctg ggtcaggccg taaccgatat acagaccgtc attgtttacc
    57001 ccactcgtca gacgatagtc gtaagtgccg acggccgctt tttgcccatt ctgctgaata
    57061 tcacgttggg cactgctgga gaggatttgc ccatttttat cctgcaattg cagattgccc
    57121 gcatcgccca gaacttccgc accttgcgca tcaaccaact taatggtgct gttaccatca
    57181 tcgacttcag tgagcgataa cgcagagtca atatcacgag atactgagct tacaacgtca
    57241 ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca gcgttttact aacgttgacc
    57301 gtcccttctg tcatctgtgc acctgctgtc agggcaccaa aatcaacggt tccgccggag
    57361 aatcccaaag tgctaatgga gtgagcgccc ttacccacta ttacctggct tccctgaccc
    57421 gcagtgatcc cgcctctctg cagaaactca gcattctttt ccaggttaaa tgtcgtgttc
    57481 tggaacgcca gtgaaccctg gaaccagtct gtataagcaa actcatcgaa ggagaattcg
    57541 tgattcccca tgtttatttc aacaatacct ctaccttgaa gatcgttatc aaatacccaa
    57601 tctttattgg cattgatttg cagtttgcca tcaacatcca ggtccgcacg gccgatgttc
    57661 ttgtcttcag aaataaccac gacagcacca gaagcgacat caatatatcc gctgaatgca
    57721 tagttattgc cggtcagtgc cactgtccca ctgtcgatac ttattgtacc attgccggaa
    57781 atcttattgg caaaggtcat gctatcgtca gccttcaggt gtaatttccc gcggttgttg
    57841 atctctgctg tacctatatt ttgggtgatc cctaaagcgc cattttgctc aattttgact
    57901 ttaccactaa aggcagagtt atcaccggtt aagtaagtcg tgccattgag cgcattgata
    57961 tagcccaggc cctcgctgcc actgacaata tccgcggaaa tagtcgcgtt tttatccgtg
    58021 tggtttaagg tgattttctt atcggatgtc tttgcccaca caaactcaat tttttcggca
    58081 tcaataatcc ctgcagcctt cgcagcactt ccttcctgtg cacctaacgc taactcagag
    58141 ttggtgctta aactaatttt cggtgcggat attttacctc catctgatac gactaatgtt
    58201 gtatcagaac gttggtcgta ataacccagg ctaatatgtt cacgagcatt aacttcaccg
    58261 ccgttattaa ctgacaggta ctgattacca ccgtaactgc cgtaaacgcc cataaagaag
    58321 gttttagaat ttacctggct gccttcacca ctgacatttc cctgcaaata ggtcagacgg
    58381 ctggtgccct taacatggat tttgccgccg ttggagacat taaaatagcc gcgatcgtgt
    58441 ctcatgcctg agtttgactc atacaaatca tcagcatata actcgctgcc agcattggag
    58501 acattcagga accccgtacc tttcagcaaa aagttaagtg ggccattgat agttaccttt
    58561 cctccggtaa tatccaaccc accaccagaa gcgagagtca gacgcggacg ggaagtatct
    58621 gtcgcattga ttaccagatt ggcattgtca gtcaccgtaa tacggtaatc gatgctctgg
    58681 ttctctttgc caaaatcaat agttttatcc tggtttacga tgacatcatc ggcgaatacc
    58741 agagatgaat ataaacttat cgcgctaatt aaaacagccc gggacttttt tccttttact
    58801 ctgctcccca gctccgagca gacgctccaa cagttacggg acctgttcca ctttagccga
    58861 tatattttat tcatcacaat ctccagcaat acatccaaat aaaaccaaga ttgcataaac
    58921 agcaacgcat taaatgccat tctggttact gttcatcgca tataaaaatt caaaaatttt
    58981 gcatagcatt ggcaaccaca tatctgagta tgtagttcag agggtaataa aatagcaagt
    59041 tattacttaa aacttatttt aagttaacac tcttaacttt atcattacat tc
//
LOCUS       DAPHDX010000007        58060 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPHDX010000007 DAPHDX010000000
VERSION     DAPHDX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.007"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..801
                     /locus_tag="RH058_000458"
     CDS             85..801
                     /locus_tag="RH058_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3136098.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSMPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            831..1331
                     /locus_tag="RH058_000459"
     CDS             831..1331
                     /locus_tag="RH058_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3136099.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1423..4107
                     /locus_tag="RH058_000460"
     CDS             1423..4107
                     /locus_tag="RH058_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDV3136100.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4104..5192
                     /locus_tag="RH058_000461"
     CDS             4104..5192
                     /locus_tag="RH058_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDV3136101.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5280..6578)
                     /gene="exuT"
                     /locus_tag="RH058_000462"
     CDS             complement(5280..6578)
                     /gene="exuT"
                     /locus_tag="RH058_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV3136102.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7061..8473
                     /gene="uxaC"
                     /locus_tag="RH058_000463"
     CDS             7061..8473
                     /gene="uxaC"
                     /locus_tag="RH058_000463"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV3136103.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8488..9975
                     /gene="uxaA"
                     /locus_tag="RH058_000464"
     CDS             8488..9975
                     /gene="uxaA"
                     /locus_tag="RH058_000464"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV3136104.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10058..10609
                     /locus_tag="RH058_000465"
     CDS             10058..10609
                     /locus_tag="RH058_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV3136105.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEVSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10614..11858)
                     /gene="sstT"
                     /locus_tag="RH058_000466"
     CDS             complement(10614..11858)
                     /gene="sstT"
                     /locus_tag="RH058_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV3136106.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12474..13439)
                     /gene="alx"
                     /locus_tag="RH058_000467"
     CDS             complement(12474..13439)
                     /gene="alx"
                     /locus_tag="RH058_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV3136107.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13722..14708)
                     /locus_tag="RH058_000468"
     CDS             complement(13722..14708)
                     /locus_tag="RH058_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3136108.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14787..15470)
                     /locus_tag="RH058_000469"
     CDS             complement(14787..15470)
                     /locus_tag="RH058_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV3136109.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15547..16050)
                     /locus_tag="RH058_000470"
     CDS             complement(15547..16050)
                     /locus_tag="RH058_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV3136110.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16135..17271
                     /gene="rlmG"
                     /locus_tag="RH058_000471"
     CDS             16135..17271
                     /gene="rlmG"
                     /locus_tag="RH058_000471"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV3136111.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRNGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            complement(17389..19407)
                     /gene="fadH"
                     /locus_tag="RH058_000472"
     CDS             complement(17389..19407)
                     /gene="fadH"
                     /locus_tag="RH058_000472"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV3136112.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQESNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19733..22084)
                     /gene="ygjK"
                     /locus_tag="RH058_000473"
     CDS             complement(19733..22084)
                     /gene="ygjK"
                     /locus_tag="RH058_000473"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV3136113.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22101..23171)
                     /gene="ygjJ"
                     /locus_tag="RH058_000474"
     CDS             complement(22101..23171)
                     /gene="ygjJ"
                     /locus_tag="RH058_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV3136114.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23305..24738)
                     /locus_tag="RH058_000475"
     CDS             complement(23305..24738)
                     /locus_tag="RH058_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3136115.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDSMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24801..25250)
                     /locus_tag="RH058_000476"
     CDS             complement(24801..25250)
                     /locus_tag="RH058_000476"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV3136116.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25247..28339)
                     /gene="ebgA"
                     /locus_tag="RH058_000477"
     CDS             complement(25247..28339)
                     /gene="ebgA"
                     /locus_tag="RH058_000477"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV3136117.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28523..29506)
                     /gene="ebgR"
                     /locus_tag="RH058_000478"
     CDS             complement(28523..29506)
                     /gene="ebgR"
                     /locus_tag="RH058_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV3136118.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29725..30057
                     /locus_tag="RH058_000479"
     CDS             29725..30057
                     /locus_tag="RH058_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV3136119.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30099..31478)
                     /gene="ygjG"
                     /locus_tag="RH058_000480"
     CDS             complement(30099..31478)
                     /gene="ygjG"
                     /locus_tag="RH058_000480"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV3136120.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31896..33416
                     /gene="aer"
                     /locus_tag="RH058_000481"
     CDS             31896..33416
                     /gene="aer"
                     /locus_tag="RH058_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV3136121.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33570..34193)
                     /gene="nfeR"
                     /locus_tag="RH058_000482"
                     /gene_synonym="yqjI"
     CDS             complement(33570..34193)
                     /gene="nfeR"
                     /locus_tag="RH058_000482"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV3136122.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34470..35234
                     /gene="nfeF"
                     /locus_tag="RH058_000483"
     CDS             34470..35234
                     /gene="nfeF"
                     /locus_tag="RH058_000483"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV3136123.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35288..35363)
                     /locus_tag="RH058_000484"
     tRNA            complement(35288..35363)
                     /locus_tag="RH058_000484"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35328..35330),aa:Ile,seq:cat)
     gene            35488..35994
                     /gene="mug"
                     /locus_tag="RH058_000485"
     CDS             35488..35994
                     /gene="mug"
                     /locus_tag="RH058_000485"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV3136124.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36072..37913)
                     /gene="rpoD"
                     /locus_tag="RH058_000486"
     CDS             complement(36072..37913)
                     /gene="rpoD"
                     /locus_tag="RH058_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV3136125.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38108..39853)
                     /gene="dnaG"
                     /locus_tag="RH058_000487"
     CDS             complement(38108..39853)
                     /gene="dnaG"
                     /locus_tag="RH058_000487"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV3136126.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(39964..40179)
                     /gene="rpsU"
                     /locus_tag="RH058_000488"
     CDS             complement(39964..40179)
                     /gene="rpsU"
                     /locus_tag="RH058_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV3136127.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40416..41429
                     /gene="tsaD"
                     /locus_tag="RH058_000489"
     CDS             40416..41429
                     /gene="tsaD"
                     /locus_tag="RH058_000489"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV3136128.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41472..42935)
                     /gene="ttdT"
                     /locus_tag="RH058_000490"
     CDS             complement(41472..42935)
                     /gene="ttdT"
                     /locus_tag="RH058_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV3136129.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(42984..43589)
                     /gene="ttdB"
                     /locus_tag="RH058_000491"
     CDS             complement(42984..43589)
                     /gene="ttdB"
                     /locus_tag="RH058_000491"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV3136130.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKMVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43586..44497)
                     /gene="ttdA"
                     /locus_tag="RH058_000492"
     CDS             complement(43586..44497)
                     /gene="ttdA"
                     /locus_tag="RH058_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV3136131.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            44704..45636
                     /gene="ttdR"
                     /locus_tag="RH058_000493"
                     /gene_synonym="dan"
     CDS             44704..45636
                     /gene="ttdR"
                     /locus_tag="RH058_000493"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV3136132.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45649..46266)
                     /gene="plsY"
                     /locus_tag="RH058_000494"
     CDS             complement(45649..46266)
                     /gene="plsY"
                     /locus_tag="RH058_000494"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV3136133.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46371..46739
                     /gene="folB"
                     /locus_tag="RH058_000495"
     CDS             46371..46739
                     /gene="folB"
                     /locus_tag="RH058_000495"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV3136134.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            46830..47651
                     /gene="bacA"
                     /locus_tag="RH058_000496"
     CDS             46830..47651
                     /gene="bacA"
                     /locus_tag="RH058_000496"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV3136135.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(47814..49052)
                     /gene="cca"
                     /locus_tag="RH058_000497"
     CDS             complement(47814..49052)
                     /gene="cca"
                     /locus_tag="RH058_000497"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV3136136.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49116..49736)
                     /locus_tag="RH058_000498"
     CDS             complement(49116..49736)
                     /locus_tag="RH058_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV3136137.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            49978..51279
                     /gene="ygiF"
                     /locus_tag="RH058_000499"
     CDS             49978..51279
                     /gene="ygiF"
                     /locus_tag="RH058_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV3136138.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51302..54142
                     /gene="glnE"
                     /locus_tag="RH058_000500"
     CDS             51302..54142
                     /gene="glnE"
                     /locus_tag="RH058_000500"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV3136139.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54190..55623
                     /gene="hldE"
                     /locus_tag="RH058_000501"
     CDS             54190..55623
                     /gene="hldE"
                     /locus_tag="RH058_000501"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV3136140.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(55743..55802)
                     /gene="ibsE"
                     /locus_tag="RH058_000502"
     CDS             complement(55743..55802)
                     /gene="ibsE"
                     /locus_tag="RH058_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV3136141.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56119..56178)
                     /locus_tag="RH058_000503"
     CDS             complement(56119..56178)
                     /locus_tag="RH058_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3136142.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(56417..58045)
                     /locus_tag="RH058_000504"
     CDS             complement(56417..58045)
                     /locus_tag="RH058_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV3136143.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
BASE COUNT        13849 a        14873 c        15494 g        13844 t
ORIGIN      
        1 aatgcttttt tatattgact tacaataaat ctacatctct aatacaccaa ctttttggaa
       61 acaatattgt aagatcaaat ttatatggtg gcgaaaatta atctcttatc acggcttacg
      121 ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa atatgactgt ttatcctatg
      181 gctgtttcca ttaatagtca gggtgaaggc aacgttcgcg taatatctaa aagtaatgaa
      241 gttcagtaca tcaaggcgac agtattccgt atcgataatc catcgatgcc tcaggaaaat
      301 gaagttgaga ttaaatccgg tgatgcaaat catctggttg ttatgccacc taaattcgct
      361 ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga tggaaccaga gcaaaaagag
      421 aaaaattatc gcgttaaatt tgaagcggtt cccagtattg atgacgttgc cacagataaa
      481 aaagatctct ctatgcagtt aacagttaac ttaatttggg ggattgttgt tagtgttcca
      541 cctcagcaac ctattgctaa gttagaagta aatgctgccc aaaaattagt taatgcaggg
      601 aatcaacgct taaagatttt aacgatcgct tattgtaaaa ataatagcaa agaaaattgt
      661 aagatacaga ccgtaaataa aaacatcttc cctggtcagg aaagaaatct tgaaagcata
      721 tctggctacg acaaaatcgt tgtcaaatat aataactgga tcaccaaaga taatggcgag
      781 tttgaactgg cggtccatta attatcattg taataaacga aggaatagta atgaaaaagg
      841 tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc gcattggcag
      901 tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg acacagaccg
      961 ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag ggtctgcaat
     1021 cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta aaaatgcagt
     1081 tagtttcccc ggcgcaactg gttcaaagcc tggatgctag caagattgtt ccgctgacag
     1141 taacctgggg cggtgaagaa attaaagctg atgcggcaac cacttttacc gcaaccaaaa
     1201 tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg tttgctcaaa
     1261 ccaccaaagg tgttctggag acaggcatct accgtggtgt agtaagcatt tatctgtctc
     1321 aggatatcta atccagataa cagaattaac acgtaacaaa gggaggggta gccctccctt
     1381 attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa aaaattactg
     1441 gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa aattcccgca
     1501 gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt ttcagggaaa
     1561 agtctcggta aatattatgc aatggttaat cttgataccg taacatttct tgatccagca
     1621 agtctatata acaagctgga actggatgta gacgatcaga aaatcgcgca tatagtgaaa
     1681 gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta tgtacgtact
     1741 gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa tgatgaagaa
     1801 agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc gaagtcatta
     1861 tatttaaatc cagacaaaaa tactgttaat gcttttatac atcagcaaga catcaatgtt
     1921 ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct gggaataaca
     1981 gaaaatagtt atattggtgc acactggaat ttcaacggtt atgatgcaga tgatgtcagt
     2041 gacagtaatg ctgacgtcag cgatctctat tatcgttatg attttttacg tcgttattat
     2101 gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg gaactttacc
     2161 tttaactttc tgccactcgg tgcaattgat gggatgcgta tcgggtcgac actgagctat
     2221 ttaaaccagg cgcaaagcca gcaaggaacc ccagtaatgg ttctgctttc gcgcaattct
     2281 cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa tagtggttcg
     2341 caatttattg ataccagttc ctttccgcct ggcagctaca gcgtggcatt aaaagtctac
     2401 gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg cggtctgact
     2461 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
     2521 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
     2581 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
     2641 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
     2701 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
     2761 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
     2821 ttaagctgtt atgaaagtat ttccgcgacg gtttcacaga attttgtcgg ctggaatatg
     2881 atgctgggtt atacccgcac acaaaataac actgatgata gtttgcgttg ggataaacag
     2941 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
     3001 caacttagcg cttcccgcgc ttttgtgatg cgtgactgga ttttgagtac gtcagtcggt
     3061 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
     3121 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac aaatgtgtct
     3181 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
     3241 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
     3301 gataccataa acagcgcggt taacgggcgt tgggatgggc aatacggaaa tgtctacgct
     3361 accgtatctg atagttatga ccgtaagaat catgatcatc tttcggcctt tacgggcact
     3421 tatagctcta cgctggctgt cagtcgctat ggcgttaatt tgggagccag tggtacagac
     3481 gatttgctgg gtgcggtact ggtggatgtg aaaggcttct ctgaacagga tgaagagagt
     3541 caggatctgc aactcgaagc gcgggtggca ggcagccgaa ctttgcagct tggtcaaagt
     3601 gacagtgtgt tgttccctta tcctggattt cagtctggtt ttgttgaggt taacgacagt
     3661 agccagggca atcagcaggg gacaacaaac atcattaacg gtgcagggaa tcgtgaatta
     3721 atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg ccagctttaa ctacaactat
     3781 atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc cgattgtggg gctgaacagc
     3841 gccatgttac tggtagctga agatggcgga tttacacttg aaattaacgg cagcgaaaaa
     3901 gagctctatc tgctttccgg gcagcaattc cttaagtgtc cactaagtgt tgtaaagaaa
     3961 cgcgccagca ttcgttacag cggagatgtt acttgtagtg tggtgactta ttcacaatta
     4021 ccggagtcga ttcaggttca ggcacagttg aaacagccta aattacgtgg aaacgttcag
     4081 acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcggcgata ttgggcttgt
     4141 ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt acgtatgatt
     4201 tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca ggaaataatg
     4261 gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca gctaatggaa
     4321 atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt tggccggtaa
     4381 aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcca ggatgcaagg
     4441 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
     4501 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
     4561 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
     4621 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
     4681 tgttcttccc ggagttcgcc cacgccacgc caagggtgca gttagacttg catccaacag
     4741 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
     4801 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
     4861 ctgcagggcg cagagacggt tacttctcta tctatcgtca gggagggacc accaccgacg
     4921 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
     4981 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaacgt gtgcgtccgg
     5041 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg actctggcag
     5101 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
     5161 tctttacgcc ttccttgcca accatcaatt aaccctgcaa accggacaaa cgcttttttc
     5221 tatataaacg acgaagccgt ctgataaggc ggcttcgtca ttacaactta agttaaacgt
     5281 taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc
     5341 tgcaacacgg tccagataac cagcgcacct aacaggtcaa atactgccag aactgcgaac
     5401 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt
     5461 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
     5521 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa accaccgata
     5581 cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc cgggccaatc
     5641 atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
     5701 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
     5761 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
     5821 aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa acgcggcagc
     5881 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
     5941 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
     6001 tgcttctggt cgcgtggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
     6061 aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc ccataccacc
     6121 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc taccgcgatg
     6181 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
     6241 gcttccgcgg caccgactgc accacgagca atcgccaggc caccccagct accagccagc
     6301 gcggttgcac cacagaacac ggcccacagc acagcaaaca ttgcataacc gattttcgta
     6361 cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc agcagaatag
     6421 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
     6481 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag cgtcaccagt
     6541 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
     6601 tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt tacgaccggg
     6661 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
     6721 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
     6781 cgacttatga tttgcgacgg cagaaagata acttgtcata caactttaaa aggtgagagc
     6841 catcacaaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
     6901 cgcggcctgc agagagattt accactttga gagtaatttt tttaactacg tttattgatc
     6961 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
     7021 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
     7081 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
     7141 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
     7201 taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg ctatgcgtac
     7261 caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa aatttgacgc
     7321 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
     7381 actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
     7441 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
     7501 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
     7561 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
     7621 caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
     7681 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
     7741 tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc
     7801 tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg gcgaacccct
     7861 gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg gtgccgaata
     7921 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
     7981 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
     8041 ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc cgaaaaccat
     8101 tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg gtaacttcca
     8161 gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
     8221 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga gccgctttgt
     8281 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
     8341 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
     8401 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
     8461 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
     8521 gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga taaccagact
     8581 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
     8641 ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggcg
     8701 ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
     8761 tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt gcagatctat
     8821 cgtcgcgcta acggcgatgt cggggtgcgt aatgagctgt ggatcctgcc aaccgtgggc
     8881 tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac caacaacgcc
     8941 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca actgggcgac
     9001 gatcacatca acacccgcac catgctgcaa aacatggtgc gccacccgaa cgcgggcgca
     9061 gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg
     9121 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
     9181 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
     9241 ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
     9301 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
     9361 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
     9421 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt caaacagtac
     9481 tttattgccc atgaccagcc gatctatgaa aacccatcgc cggggaacaa agcgggcggt
     9541 atcaccacgc tggaagataa atcccttggc tgtacccaga aagcgggttc cagcgtcgtg
     9601 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccgg ggctgaactt gttaagtgcg
     9661 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
     9721 ttcagtactg gtcgtggcac gccatacggc ggctttgtac cgacggtgaa aatcgctacc
     9781 aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg tcagctgatc
     9841 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgtgtttgcc
     9901 aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
     9961 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
    10021 gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt ggctggccca
    10081 gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaacg
    10141 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
    10201 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
    10261 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
    10321 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtacctg
    10381 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
    10441 ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg aggttagttt
    10501 tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa ggggaattga
    10561 tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa caattaatta
    10621 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
    10681 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gatgataaag
    10741 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagc
    10801 gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
    10861 accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt attaaccgca
    10921 gccagcgtca acacggtaat ggtgattgct gcgccagcca tattgatggt ggcacccagc
    10981 ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag tgccatattc
    11041 accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct ttcgcgcagg
    11101 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
    11161 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
    11221 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa aataccaatc
    11281 ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat atcgttgacc
    11341 aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat tgcccacacc
    11401 aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac catgctcatt
    11461 accagcccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc cgcgctactg
    11521 gataaatgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag agcagcagag
    11581 aaggtgccca gtagatagag gaacaaaata gggcggatat tggttttctg cccgtgctgg
    11641 tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac ggctttcagt
    11701 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
    11761 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct gccatgagcc
    11821 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
    11881 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
    11941 aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat tttaaagaga
    12001 ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg aaggactggc
    12061 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
    12121 tcctttaatg tgttatgcca tataaagtca ctggcgcagg gagcgcgcag ggggcggcca
    12181 atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct gcgcggttcc
    12241 ctcagcttat cccttgcggc taccgggtcg ggcgcaaggt aacgtccctg taagatgcgc
    12301 cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg cgattttgac
    12361 gccaccaaca cccgtgcggt ctttttgtaa agagctataa gccgattaca taattaatag
    12421 ctcacctttg tctggaatga gttatctcta gtaatggctt aatatacagc ggatcattct
    12481 ccccgctgct tatcatgccg ataattcacc caggcgttga taataaacgt catcaccaga
    12541 atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc gacaatcagc
    12601 atcttgatac cgataaacac cagaatcacc gccaggccat atttgagcat cgagaaacgc
    12661 tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc aaacaggttt
    12721 gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat gctatccacg
    12781 gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg tgtggcgtac
    12841 agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa
    12901 tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg
    12961 agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag gatccagtcg
    13021 aactgtgaaa tcagccagct gcctgtgaag atcatgatgg tacgcagaac aatcgccccg
    13081 agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa atagctgaac
    13141 aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag atagcccgtg
    13201 agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cagtttgcac cagataccac
    13261 cagaaagcgg cgttaaacag taacgacagc gtcacccaga ccagcgacca ggccgcagcc
    13321 tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc gatagccagc
    13381 ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac agtattcata
    13441 gaagttcctt acacataaaa aaacggccaa cgtcggaaga cgtcagccgc tgctttttat
    13501 gcatagacct cgccttccgg caaggtctca cttacaacaa aaaaggaata cgtctgcgta
    13561 ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat gacgatcgac
    13621 cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagaccatt cagccagtca
    13681 atggtctgtt acataccttt tacactgttt tactttggca attatagttt tacgctatct
    13741 gccggaaaaa tcaccccagt ctggcggcgg atctcggtca gcagtttggc ggtgatgcga
    13801 ctgaccgcca gccccggatg atccaccaga tgctcatcca ccagggtagc gaacagctct
    13861 gcttcgtaga gcatggtatt aatatgctgc ggctgggtga gatcctgcat ttggctgcca
    13921 cgcggcacga agcacacttt ctggcattca gacagttttt caatcaccag cgatcctgct
    13981 tcgccctgaa tctcgctcgc caggacagaa tcactgactt ttgagtgctg caaggtgacg
    14041 ctgaaatcac cgtaatccat caccaccacg ccgtgggcat ctacaccgct tgccagcaaa
    14101 ctggcggttg cctgcacgct tttcggctca ccaaataacg ccaccgccga cgccaggcag
    14161 taaaagccga tatccataat tgaaccgtta gagaatgacg gattaaaggt gttgggattt
    14221 tcaccgtcca ggtaacgttg atagcgcgag gaatactggc aatagttgaa aaagacttta
    14281 cgcaatttgc cgactttcgg cagcgcctgg cgcagcaaat gaaagttcgg caggcaggcg
    14341 gttttaaatg cctcaaacag caccacctga ttttcccgcg cacaggcaat ggcggcatcc
    14401 acttccgcca gattcgacgc cagtggtttc tcgcaaatca catgaatttt atggctaagg
    14461 aaaagttgtg tctgggaaaa gtgcagggca ttcgggctgg caatatacac cgcgtcaatg
    14521 gcatcgcttt ccgccatcgc ttccagcgag gtaaacagat gctcgacaga aaaatcattg
    14581 gcgaagtgct gagcctgctc aaggctgcgg gaatatacgg cggttaactt gtatttaccg
    14641 ctctcatggg cggcctcgac gaactggcga gtgatccagt tcgtaccaat cacagcgaaa
    14701 cgtatcataa cgcgtggata ctccataaag ggattctgtc gtcaatgtag cacgcctttt
    14761 tcatcttcga cgtgcgcaga tacgcattat tcttttgccg gacagccatg ctcactaaac
    14821 ggcccaatca tgaccgatgg cccgagatat ttcggctggc gatggaggat tttggcgtca
    14881 atcacggcgc tgacacgagc gagtttctcc cgcagttgag tataaaaacc gtgcttcata
    14941 ttgaggtcag gcgcgttaaa accgatagcg tcgatgccgt actgttttgc cagccagatg
    15001 gcgcgttgat tatgaaattc ttgcgagata atggtgatat ggttttcacc aaaaaccttt
    15061 ttggcacgca ctaccgaatc cagcgttgag aatccggcat agtcgcagaa gatcactttc
    15121 gccggtacgc ctttagcgat caaagcctgc tgcatacctg aggcttcatc gtaattttta
    15181 cgtccgttat cgccacttac cagtagccat ttcacttttc cggcgtggta caacgctgct
    15241 gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc tggcccccag caataagccc
    15301 acgttacgcg ccggaactgc gttgacatcg ctccaggtca gctgcttgct ggcgttgacc
    15361 ataacgcgat cggcaataaa aatggtcgct cctgctataa gcaacaacaa acagccaatc
    15421 ttaagagtcc ggcgagaaaa acaaatacgg agaagaaggc ggggctgcat ggtcgttatc
    15481 cttaacgaaa ggggggaggt cgattttagg cgctaaagaa agaaaagata acgcctgaca
    15541 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    15601 ctcaagctgg tggtactgcg gctccatatg gcagcacaac tggtaaaacg ctttgttatg
    15661 ctctttctcc ttcaggtgcg ccagttcgtg caccacgatc atacgtaaaa acggttccgg
    15721 cgcgttgcga aacacagtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    15781 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    15841 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    15901 atattgccag agggctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    15961 cacatcgccc agacgctgtt cgttaatcag cgttcgcacc tgggaaagta gctgctcggg
    16021 ataaccctga agataagtaa gattgttcat aaccgcccac gaaaaagaga aaaagggtat
    16081 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    16141 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    16201 aacccgctac aggcgtggga agcggcggat gaatatttgc tgcaacagtt ggacgacaca
    16261 gaaatccgcg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    16321 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    16381 aatttacgcc tcaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    16441 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    16501 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    16561 aaagcccgtg acattcacac ttccacgctg gagctgttcg agaaagtgct cggcccgacc
    16621 accactacgc tggcatggaa aaaagcgcgc ctgattaatt gcactttcaa tgaaccgccg
    16681 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccac
    16741 aaccatgcga atgtcttctc ccgcaacggg ctggatattg gcgcacgctt ctttatgcaa
    16801 catctgccag agaatctcga aggtgagatt gtcgatctcg gctgtggtaa cggcgttatt
    16861 ggcctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    16921 atggcggtcg cttccagccg aatgaatgtt gaaaccaaca tgccagaggc gttggatcgc
    16981 tgcgagttta tgatcaacaa cgcgctctcc ggagtggagc ctttccgctt taatgctgtg
    17041 ctctgcaacc cgccgtttca tcagcaacat gcgctgaccg ataacgtcgc ctgggagatg
    17101 ttccaccacg cccgccgctg cctgaaaatc aacggcgaac tgtatatcgt tgccaaccgt
    17161 cacctggatt acttccataa actgaagaag attttcggca actgcaccac catcgccacg
    17221 aataataaat ttgtggtgct gaaaacagtg aagctggggc gtcgtcggta agattgggag
    17281 ttacctgccg gatgcggcgt taacgcctta tccggcctac gttcagcacg ttactcccag
    17341 gccggataag acgcggcaag cgtcgcatcc ggcacttcaa cgcacttttt aaatctccag
    17401 cgccagccgt gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca
    17461 gccgccaatt aaatgcaccg ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt
    17521 tgactcttgc cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt
    17581 gatcaccaca tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt
    17641 cacaccccgc gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg
    17701 tcccggtttg ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg
    17761 cattccctgc gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt
    17821 acagaacctg gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt
    17881 atcaaaacca atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc
    17941 gcgcagtaca tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac
    18001 gcgcggcacg atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt
    18061 cacggtgtga ttgagtttca gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg
    18121 cagcgtttcg taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc
    18181 gatctcgctg tgcgcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc
    18241 aaacgccagc ccagcaggtc cagcgccgac caccgccaga ttttttttct gcacggcggg
    18301 aaggattggc attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt
    18361 gccaacgaag atctgatcga ggcaggcctg gttgcagcca atacaagtgt tgatctcatc
    18421 ggctcgccct gattgcgctt ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat
    18481 cgatactata tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat
    18541 ccggttggtg gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct
    18601 aaatgcgccg cgcggcacgg gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat
    18661 tccggtgttg ataatggtcg cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc
    18721 ggcaaaagtg ccgccgcctt cgaccaggtc gagcatcgac aaccggtaga taataatgaa
    18781 gtcgttgccg acgcgttcgc gcaccgtacg cactacttct acggcaaatc gcatccggtt
    18841 gcggtaatcg ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt
    18901 gatcaaatac ccttcggacc ccattacctc tacaccgtcg tatcctgcct cccgtgccag
    18961 ttgcgcgcag tgggcgaaat cgtcgatcag ttgcaggatc tcttcatgcg tgagttcgtg
    19021 aggaacgaaa cggttgatgg gggcctgcaa tgcggaggga gcgaccagat gcggttggta
    19081 gctgtagcgc ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtaccgc
    19141 ttcggtaatg gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc
    19201 catgccaacg cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg
    19261 acgggcgcgt tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag
    19321 cccggtgtgc attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag
    19381 cggggcgaac agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt
    19441 ggtcggatga gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt
    19501 tgtgatggga ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgcgt
    19561 tttatccggc ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc
    19621 agtggcacgg tgcagatgcc ggatgcgacg cttgtcgcgt tttatccggc caggaggatt
    19681 gcacatgtag ggcggataag gcgtttacgc cgcatccgcc agcagttgca aattattgct
    19741 tacggaaaaa atcgttatac aacatataca aatgcgcggc actccaggag aaatttggtg
    19801 cgccttgctg cgcgccagtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    19861 atcctttggc gtgccggaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt
    19921 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt
    19981 agatatcagc gccaaaagct ggattggtta acgccgccgt tcccagcggg acaaaggtgt
    20041 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg
    20101 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    20161 ccgcgcagcc gttcgccagc ggtttatctt caatacgcac gtcatagaag tactgtgtag
    20221 ccgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    20281 gcttagcttc ttccggttta ccgagaatcg tcgccatctc cgccagataa tggttatcgc
    20341 tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    20401 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    20461 cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacgcc
    20521 ccgattccca cgatgcagca acctgtgccg gaatttccag actgtcgtac tggcctttct
    20581 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    20641 ccgtaaacag catctcgccg ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt
    20701 ccggcacgcc gttgccgttg tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    20761 gtttcgggta catctctgcc acccaggttt tatcttgggt gacgttatac acttccatca
    20821 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    20881 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    20941 gcacgcggtc gcctggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    21001 tgtccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    21061 gattgccgga gaaccagcgc ccggtcaccg acggtgtgac ggtgttaaat ttcaccgccc
    21121 cgccaggcga acgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    21181 gttccggcgt cgcatccgga ttggttaacc ctttcttcag atactcttcc cagcgttgct
    21241 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    21301 tgctaacttc ctgagaggtc agcagatggg aataggtggt atagagcgtg gtcgaaccgt
    21361 tgatatgtgc cttactggta aagcgattgc cgttgatttc agtctgcacc ggcagggatt
    21421 tatgcacctg atattctgat tcaccggagg tcagcagatc ccaggtggcg cgcactttgc
    21481 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctggtag tcagggtatt
    21541 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    21601 gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt tccagtagtg
    21661 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacttg cacatctttt gctgtcagtt
    21721 tttgcaccag cgcaccggga atactgtatg cctccagcgt aaagtcgact ttcttgccgt
    21781 cctgccagac ggtcaggcgg tcgaaattgc tggccataaa gttgatgtac tcttccgtca
    21841 gcagcgcaac gcccggaaag ccgcccatgg tgttagggcc gtctggcaac agatgaccat
    21901 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    21961 tcatgtactg cggcgcgcca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    22021 catgggcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gtttttattt
    22081 tcatgaaatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    22141 ggatcgtgag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    22201 ccgccgtatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    22261 tcagggcgat gtttggcgta ggtataagcg gtggagagat aaacgccctt taacgcttca
    22321 tacatggcgc tcaccatcat cccactttca cttcccggta cgtccagccc gtcggcgcgt
    22381 ccggtatggt gccaggcgcg agcggcaaag ttaggagtta gcaggcccgt gagatagagc
    22441 tgtcctgtgc cgtcggtgat ttccagaccg ttcatccagg tgatgccttc ggcaaggtca
    22501 tactggatgt aaccgttcat cattgccgga taggtgtatt tgtcgtcata gcgatcgtac
    22561 ttgccaaaat gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg
    22621 ctataacgaa agttgccgct taagttctgc accttcagcg cggtcataat gtcgcgggta
    22681 ttgggaatga cgtagcccag atcgggggtg aagtcgcccc accattgcag gtcatccagc
    22741 gaggaatcct gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca
    22801 tagaaacggt taattccgcc ctcgaaacca ccccagctat ggtctggcac ccaggcattg
    22861 ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg
    22921 tacttcatct ccaggccacc ttcaatatag gtaccgtcgc tgtatttatg tttattctca
    22981 ccctctaaat ccatatatcc accaacccct aattcgccgt aaaaacgaaa tgaaggagaa
    23041 gatgtcgagg taattgcagg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct
    23101 cctgccgcag aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg
    23161 attaatttca ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa
    23221 ttataaaaat ataaaggcag aaactatttt ccctctcctc atccaggaag agggaaaatg
    23281 cctgctcatt ttttattatt ctgcttaatc aacattgctg gctggcgtcg cttcaatatg
    23341 gcgctcttca gccgttaatc cctttatata tttactgtat ttccaccacg caaagccgag
    23401 gaagataaca ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg
    23461 gaatgtcgaa gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat
    23521 accggtgcgg cgggagccca tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa
    23581 attcaggtaa gccagcatga tgaataacgg cggaagcatg gacgctgcgg cggtcatatt
    23641 aataatagta ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag
    23701 cgggatgacg atcaggaact ggatccacgc tgcgcgcgcc ggaacgccgt tttcgttcag
    23761 ttcgacggtt ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg
    23821 cgttgcggtc cacatcagca gggaaccgaa catcgcggta aaggacacca gaccgacaaa
    23881 gcgattcatc aacgcttccg gtagaccaaa atacgccgcc atgccgtgga atacctgcac
    23941 cgatccgccg gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga
    24001 gacggaatac agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt
    24061 cgaaccgcct ttgacgtcgt taacgtacac agcgacggac tccgcgccac ctgcggccat
    24121 aaagatccag gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgagtcaac
    24181 ggtgatgggg tcagcaggct gtacgccgcc aaccagcgcc gtacctgcca gtaaaatgta
    24241 ggagagcgtt aacagcagca tcagcgttga agtgacggag gtaattggcc ccaacatttt
    24301 cgccccgttg gtggaaaccc aggtggagaa ggcgaacagc accatactga taatggtggt
    24361 ggcaaccggc gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac
    24421 gcgcggcaac agtgaggtga aaaagaacag gtttacgaac cagtaggtat aggcagtaat
    24481 aaatgcccaa cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt
    24541 tttatttaac gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt
    24601 cgcgaggaaa aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt
    24661 gttaaagctg taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt
    24721 acgtttggta tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac
    24781 tccttttgcc gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg
    24841 agaaccactt ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa
    24901 ataacgattt gcccttcgtg gacctcaacg gtttctccgc agccttttaa atattcacgg
    24961 tcagtttcat cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt
    25021 ttttgctgcc cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc
    25081 gcatcggtcg cggagtctgt ctccacgcgg taagtcaatg agtcaccgat ggagtgggcg
    25141 acgccaggct gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg
    25201 ccagaggcgt aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct
    25261 cgctgtgcaa attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg
    25321 cggtagcttc tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc
    25381 agacgcgcca ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagcagct
    25441 ggtgatcgag gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag
    25501 cgtggatgtt ttcctgggta tagtgccagg cgctgaagtt aattgggcgc tgcggaacca
    25561 ccagcaaacc gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc
    25621 cgttgttctg cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat
    25681 cgatgatgtt agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg
    25741 ccacctgatc gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg
    25801 gatagtcgcc gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc
    25861 gccagatgta ggtgcagcgc atcccgaagt caaacaccgg cggggcaata actgtgcggc
    25921 tgatgatcaa cacttcacca tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca
    25981 tgatctgcaa atgattcggt tgccacagcc cttcgtactc ctgcttgtgg ttgtcgatca
    26041 ttggcttgaa gaagttgatc tttggctcgc gggtcagcag cgactcgcca ttcacctgcc
    26101 aggatgtcgg tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg
    26161 tgcagctcaa acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca
    26221 ctggttgcgc ggtgttttcc ttcagcggga actgataagt ggcgatagaa tgcccggctt
    26281 cgctgtagcg ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt
    26341 ccagctgcgg cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt
    26401 taatctgctg cgtcgcgagc gtttcacctt cgacgcgcac ctctgcgtgc agggtgtagt
    26461 catcaagcgt ggtaaaccac agtttatttt cgactttcag ctcgccgcga gtcagatcca
    26521 gcgcgtggat ttttaccggc gcgataacct gtttgtactc tttcaggccc ggtcccggcg
    26581 tctgatcgga atagatcaaa ccatcaagac agaagttata gttgttggga tagtcgccgt
    26641 agtcgccgcc gaatttatac cagacgttgc cgttgtcatc ctgcgcctgg atcccgtggt
    26701 cgcaccactc ccaaacataa tgtccctgaa tacaatcgtg cttatagaag acgttctggt
    26761 actccgtcag cccgcctggt ccgttcccca tcgcatgagc atattcacag atgatgcgcg
    26821 gcttcggatg cgggtattca ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg
    26881 aaataatatc gaccacttca gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat
    26941 ccagcgcctt cgctgcgtgg tacatcgcgc ggatgttaca gccatagccg gattcattgc
    27001 ccagcgacca gatgatgatc gacggatggt ttttctgcgc gtgaatatgg cgaacaatgc
    27061 gctcgacgta gactttttcc cactgcggat cgtcggtaat acggctgata tcgccgacat
    27121 tagcaaagcc gtgcgattcg acgtcggttt ccgccatcac aaacaagccg tagatatcac
    27181 acagttcgta aaaacgcgga tcgttcgggt agtgagcggt acgcacggag ttgatattgt
    27241 gctgcttcat caactggaga tctttctcga cgcgatccat tccaacggcg cggcctttgc
    27301 gatgatcgtt gtcgtgacgg ttgacgccgt gcagcatcac ataacggtta ttgatccaga
    27361 acagaccgtc gcgcactttg atatcacgga agccaacgcg ttgtggcacc acttccagaa
    27421 cgttgccgtt ggcgtctttc agcgtcatga ccagatggta aagataaggg gattctgctg
    27481 accattgctg cggctgttcg acagtaaaag caaagctggc gctggtcagt ttttcaattg
    27541 ccaaatgatc aatggcgctg ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca
    27601 gcgtcgtgac gacaggggag gcggcgagat tttccagcac cacttcgcag gaaagcgtgg
    27661 catcgcaata ggcttcgtca aagtcggtac gcacagtgaa atcgttaata tgcgttaggt
    27721 gttttccgac cagataaaca tcgcggaaga tccccgctga ccaccacata tcctggtctt
    27781 ccacgtaggt agagtccgcc cactgcatca cgcgcacaca caacaggttg tcgccggttt
    27841 taaccatcgc gctgatgtca aactctgcgg tcaggcgact gcccttgctg aaacccacat
    27901 actgaccgtt aacatagact tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct
    27961 gtttaccctg ccagccgtcg ctgagggtga aaatacgttg ataggcaccg gttgggttat
    28021 cgctggggac aaacggcaca tcgatgggga acggaaaacc ttcgtcggta tattgcagtt
    28081 tgccgtgacc ttccatttgc cacatggcgg ggacggtaat atgcccccag tcagccatta
    28141 actcagaggt gaaggcttct ggtacttgca gcggatggtc aaaaaagtgg aaattccact
    28201 gaccgcttaa gggcagaaac aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa
    28261 cagaatcata tgaaaaaaag tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa
    28321 tgttttccca gcgattcata gggcctcccg gtagagagaa atcggaacca ttcatggtga
    28381 tggtagtttg tgtaattgct ggggagttta gtaaaatttt tactaaaaag tagcaggtaa
    28441 atgcatttac tttaactgga tcacgaaaat aaacggcagc actgtccggc aaaaacgatt
    28501 gccggacagt gtgtaagggg atttaacggg tcgtgccgcg cagttttaat ttgctgggaa
    28561 cgaagactaa cagcggcagc gcgcgaccat cgcgggcttt ttcatacacc aggttaacgc
    28621 cctgacttcc catcatctcg gaatggatgc gcacggtgga gagcggcgga aaggtaaatc
    28681 gcgcggtggg gatatcgtta acgctgataa gcgaaatatc ctgtgggatg ttcaggcctc
    28741 gttcatgaat tgcccgcagt acgccgatag caatggaatc ggaagcaaca aacagtgcct
    28801 tcggatagtc ttcccgcgcc agcatttgtt ttgccagttc ataacccgac gaactggaaa
    28861 aaccgccgcg ccagatatcc tcttcgcgta ccacttgttt cagtcggcca tattccgcaa
    28921 aggcgacctc acgaatatcc gccttgccag gctcatcttc accgccaata aaaccaatac
    28981 gattaacgcc ctggttgata tagaagtcga tgatttcttt actgatgcgt gccagatcga
    29041 tatccaccgc atcgtaaccg ctgccgggtt cgtgaaagtc gataaaacag atattgtcgg
    29101 tcaacgcaca ggcggcggcg cgcagggcgg gcgtgggttt gccgacaatt aaaataccgg
    29161 tgacgttttt aatgtctggt aatccgctgt gttcataaca gttggtgagc tcaatgccca
    29221 gcttttcgca ctgagtttca atgccgtggc ggatcgccag atagtaagga tcgttgatct
    29281 ccagctcctg ttggtagctg tagatagcca gaatatggtg ttggttgact gcacctgtct
    29341 ggagtttacg ggcactactg gtcttgtact ccagcttttc ggcgatctcg agaatgcgat
    29401 gtttcgtctc ttctttcaca ttcaatgtcg gatcgtcatt taagaccctg gatactgtcg
    29461 ccagggatac gccagcttcg attgcgatgt cttttagtgt tgccatgttt acctttttta
    29521 ctttccctga tgtgcgcccc cattgtagag gaagtgctta tcgggtcacc tgttttcagt
    29581 aaataaagcg cgtttctttg acgctgtaac ggtaacacag cggctcacga atgtgatcac
    29641 gcctgcattc tccattttta gcctacagga ttgagaaaaa cttttgctat agtttagtaa
    29701 aattttaccc gaagggagga aacaatggaa accgtggctt acgctgattt tgcacgtctg
    29761 gaaatgcgcg tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac
    29821 atcgtacagg ttgatgtggg ggaaaaaaca ctgcaaaccg tgaccagcct ggtgccgtac
    29881 tacagcgaag aagagctgat ggggaaaacg gtggtggtct tatgcaatct gcaaaaagcg
    29941 aaaatgcgtg gtgaaacgtc ggaatgcatg ctgttgtgtg cggaaaccga tgacggcagt
    30001 gaaagcgtgt tgttaacgcc ggagcggatg atgcctgcgg gcgtacgcgt cgtataaaaa
    30061 gatcggatgg cgacgtcgta tcgccatccg atttgatatt acgcttcttc gacacttact
    30121 cgcatggccg ccagcgcctt acgcgccgct ttgatcacca gttcacactg ttcaatggtc
    30181 agtgtcagtg gcggttcaat gcggatcgtt ttggcgttat tgagcgttcc ggccaccagt
    30241 acgcgctggc ggaacatctc gctggcaaag ttatagccga tttcgttatc aacaaactca
    30301 attgccatca acatcccttt accacgcgct tcctgtacca gatcgggata ttcccgcgcc
    30361 agttgacgga aaccgtccag caacatatcg cctttttgct cagcctgagc cggtaagttc
    30421 tgctccagca acacattgat ggtcgccagc gccgccgcac aggccagcgg gttgccgcca
    30481 aaggtggtgg tatgcaggaa tgggttgtcg aacagaactg aaaacacctc ttcagtggcg
    30541 atggtcgcgc caatcggcat cacgccgccg ccgagcgctt tggcaaggca gaggatatcc
    30601 ggctgtacgt tctcatgctc gcaggcgaac atcttgcccg tgcgccccat gcccgtttgt
    30661 acttcatcga ggatcatcag tgcgccgaac tcatcgcata gcttacgtac ggcggtgaga
    30721 tagcccggcg gcggcagaat tacgccacct tcaccctgaa tcggttcgag gatcaccgca
    30781 gccacatcat caccggtttt tttgcactcg ttaagagccg tgcgcatggc ttcgatattg
    30841 ccaaacggca catgacggaa gcccggcagc agtggcataa acggtttgcg gaaggtcgat
    30901 ttcgccgtgg ccgacagcgc gccaagtgat ttaccgtgga acgcgccgct ggtggcaata
    30961 aaagtaaact tgccgcgcgg tgactggtaa gctttcgcca gcttcagcgc tgcttcaacg
    31021 gactcggtgc cgctattaca gaagaagctg tatttcagtt taccgggcgt tagcgcagca
    31081 agggttttcg ccaacatcgc ccgtaacgga tcgagcagct cctggctgtg cagcggttgt
    31141 ttcgcaagtt gattctgtac ggcggaaacc acaactggat tacggtgccc cacgttgaaa
    31201 attccaaaac ctcccaggca gtcgataaac tcctgtccct gggtgtcgac aagcgtattt
    31261 aaacctcccg cttgccactc tacggctccg taatccccgc cggcggtaac agatttgcga
    31321 tactctaaaa accccggatt gacatgctct ttgaagtatt caatcacctc tcggttaagt
    31381 gctttcatct cctcatgatc cagcgttcgc ttctcaatga gattcagggc gtgggcgctg
    31441 cacgctaaag ccgatgcgct cgaaggtaac ctgttcaaaa tatgctccgg aggctcgcgt
    31501 atcacatgat accgatttaa gtattgcagg gattgcgcca ctccggctgc gatcgcgaaa
    31561 atcgggataa acacaaattc ggttatcatt tgtgcaatat ttaatgctat ctgttaacat
    31621 ttgttgttat tttgcacggt ttttgtcggg tatggatttg atgattgctg cgcaaatgca
    31681 cgttttcagg gcaataattg cctgattttt gggcaagata attaacgttt tcgtatagta
    31741 acagagtgaa agtattctaa atcaaagaat tagatttgtg ctttaatcgc aaattgcgat
    31801 ctaaatcaaa ttaatcggtt aaagataacc gcagcggggc cgacataaac tctgacaaga
    31861 agttaacaac catataacct gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc
    31921 agcaaaatac cccgctggcg gacgatacca ctctgatgtc cactaccgat ctgcaaagct
    31981 atatcactca tgctaatgac acttttgtgc aggtgagcgg ctttaccttg caagagttac
    32041 aagggcagcc gcacaacatg gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata
    32101 tgtggttcac cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa
    32161 atggcgacca ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca
    32221 gtggctatat gtcgattcgt acccgggcga cggatgaaga gattgcggcg gtggagccgc
    32281 tgtacaaagc gctgaacgcc ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc
    32341 gtaaaggctg gctgggtaaa ctgccttcat taccgcttcg ctggcgggcg cgtggagtga
    32401 tgaccctgat gtttatcttg ctggcggcca tgctttggtt tgttgctgcc ccggtggtga
    32461 cgtatttcct ctgtgtgtta gtggtattgt tggcaagcgc ttgttttgaa tggcagattg
    32521 tgcgcccgat agaaaatgtc gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata
    32581 gtgttgagca cctgaatcgc agcgatgagc tggggctgac attacgcgcg gtagggcagc
    32641 ttggcctgat gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa
    32701 atggcagtga gacgctggcg aaaggcaccg atgaactgaa cgaacatacc cagcagacag
    32761 ttgataacgt tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga
    32821 actctgccac ggcgtcggct gccgataaac tttctatcac cgccagtaat gcggcagtgc
    32881 agggtgggga ggcgatgacc acggtgatca agacaatgga cgatatcgcc gacagtaccc
    32941 agcgcattgg caccattact tcgctgatta acgatattgc gtttcagacc aatattctgg
    33001 ccctgaatgc ggcggtggaa gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg
    33061 tggcagggga agtgcgtcat ttagccagcc gcagcgctaa tgctgccaac gatattcgca
    33121 agctgattga tgccagtgct gataaggtgc aatccggttc gcagcaggta cacgccgccg
    33181 gacggacgat ggaagatatt gtggcacagg tgaaaaacgt cacccagttg atcgcccaga
    33241 ttagccattc aacgctggaa caggccgatg ggctttccag cctgacccgt gcagtggatg
    33301 agcttaacct gatcacccag aaaaatgccg agctggtgga agagagtgcg caggtgtcgg
    33361 cgatggtgaa acaccgcgcc agccgactgg aagacgcggt gacggtgctg cattaatcgg
    33421 tatgccggat ccggcggttg gagcacaatg cctgatgcga tgctggcgca tcttatcagg
    33481 cctgcggggt gtgtagcggg tgtaggccgg ataaggcgcg tcaagcgtcg catccggcaa
    33541 ttgcaccgcg ccactggcgg atgcggcgat taatccagtt gcgtaatatc aaaagcggcg
    33601 cggtcgatca ccgcaatgat cttttttatt tgtgcatcac tgatatcgct ctggttgacg
    33661 cgtaaatcca gcaccgcttt aaaattatcc agcgcccgct tcatttgcgg gttctggcgc
    33721 agcgccgcgc caacgcaacg cgctttgatg cgttcttcaa tcatctccac ctgttcgcgg
    33781 ttttcttcca gccactgcgc gccttgttcg gtcagcgcaa tctgcttctt accaccttcc
    33841 tcttcgcgga tggtaatcag cgactgctcc tgcagaaaat ccagcgttgg gtagatgacg
    33901 cccgggcttg gggtgtaatt cccctgggtt aggttctcaa tcgctttaat caattcgtaa
    33961 ccgtggctgt catcgcgcga gagaatatcc agaatcacca gacgcaattc accgtgacca
    34021 aagaaacgtt gccgacgacc gccgccgcga ccgtggcgac caccgcagca ttgaccgtgt
    34081 tcatgctggt gaccgtgtcc gcagtgctcg tgttctgact tctcaccttt gcagcagccc
    34141 tcatggcgtg gctggccttc atgtttacaa cacccttcgt gatgatggct catatcagcc
    34201 tccttttgct tgattagata tatctaattt atatctgatg tgagcattac tgcaagtaca
    34261 aaaatgattt ttaaacgtgg aagcacctca catccaggcc ggataagacg ttcacgccgc
    34321 atccggcagc attgctatgg cactgaatgc cttcacatac ttcagatata tcagttctac
    34381 aaatcgcttg catttatcat gattaacaat cattatcatt tacgaaaatt atttagatat
    34441 atctgattac tatcacgaag gcgataacaa tgaataactc cccccgctac ccgcagcgcg
    34501 ttcgcaatga tctgcgcttc cgtgaactga ctgtgttacg cgttgagcgc atcagcgccg
    34561 gttttcagcg cattgtcctc ggcggcgagg cgctggacgg ttttacatcg cgcggctttg
    34621 acgatcacag caaactcttc tttcctcaac ctgacgctca ctttgtgccg ccaacggtaa
    34681 cggaagaggg catcgtctgg ccggaaggac cacgcccacc gtcgcgtgac tatacgccgc
    34741 tgtatgacga actacgccat gaactggcga tcgatttctt tattcacgac ggtggggtcg
    34801 ccagcggctg ggcgatgcag gcgcaaccgg gcgataaact tacggtggca ggtccgcgcg
    34861 gttcgctggt ggtgccggaa gattacgcgt atcagctgta tgtctgcgat gaatccggaa
    34921 tgcccgcact gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta
    34981 gtgcgctggt tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac cttgatggtt
    35041 ttaatatcga atggctggca catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc
    35101 aaatccctgc cgatgattac ttcatctgga taaccggcga aggtaaagtc gttaagaatt
    35161 taagccgccg ctttgaagcg gaacagtatg acccacagcg ggtacgtgca gcggcttact
    35221 ggcacgcaaa gtaactgata tataaaaaga aaaaggctga cgatttctcg tcagcctttg
    35281 ctatatctgg tggcccctgc tggacttgaa ccagcgacca agcgattatg agtcgcctgc
    35341 tctaaccact gagctaaggg gccatggtag cggattataa agtaactccg tgtcgcaatc
    35401 cagccattac cgtgcgcctg ctgtttttat aaacaatgta ttttcaatcc gttatacttt
    35461 tcctggtgat gttagaaagg agtaaatatg gttgaggata ttttggctcc agggttacgg
    35521 gtcgtgtttt gcggtatcaa ccctgggctt tcatccgccg ggactggttt tccctttgct
    35581 catccagcaa atcgcttctg gaaggtgata tatcaggccg ggtttaccga ccgtcagttg
    35641 aagccgcagg aggcacagca tctgctggat tatcgttgtg gcgtcaccaa actggtagac
    35701 cgtccaacgg tgcaagccaa tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag
    35761 ctgattgaaa aaattgaaga ttatcagccg caggcgttgg cgattctggg caaacaagca
    35821 tatgaacagg gattcagcca gcgcggtgca cagtggggga aacaaacgct caccattggt
    35881 tcgacgcaga tttgggtgct gccaaatccc agcggtttaa gtcgcgtttc actagagaaa
    35941 ctggttgaag catatcgcga gctggaccag gcgctggtag tgcgtgggcg ataaaaacgc
    36001 caccgaaaag gtggcgtttg tgcagaggga ctagtgccgg gtgcggcgta acgcctgatc
    36061 cggcctaccg attaatcgtc caggaagcta cgcaacactt cagaacggct cgggtgacgc
    36121 agtttgcgca gcgccttcgc ttcgatctga cggatacgtt cgcgggtaac gtcgaactgt
    36181 ttacccactt cttccagcgt gtggtcggtg ttcatatcga taccgaaacg catacgcaga
    36241 acttttgctt cacgcgcggt caggccagcc agcacgtcgt gcgttgccgc acgcaggctt
    36301 tcggtggtcg cagaatccag cggcagctcg agggtggtat cctcgatgaa atcccccaga
    36361 tgcgaatctt catcatcacc gatcggcgtt tccatggaga ttggctcttt ggcgatcttc
    36421 agcactttgc ggatcttgtc ttccggcatc agcatacgtt cagccagttc ttccggcgtc
    36481 ggttcacggc ccatctcttg cagcatctgg cgagaaatac ggttgagctt gttgatggtc
    36541 tcaatcatat gcaccggaat acggatggtg cgcgcctgat ccgcgataga gcgggtgatc
    36601 gcctgacgga tccaccaggt tgcgtaggtg gagaacttgt aaccacggcg gtattcgaat
    36661 ttatcaaccg ctttcatcag accgatgttg ccttcctgaa tcaggtcaag gaactgcaag
    36721 ccacggttgg tgtatttctt ggcgatagaa ataaccagac gtaagttcgc ttcaaccatc
    36781 tctttcttcg cacggcgggc tttcgcttca ccgatggaca tacgacggtt gatatcttta
    36841 acctgctcga tggtcaggcc ggtttcttct tcaatctgct gcagtttctg cagggcgcga
    36901 tgcacttctt cagagacatc gtgcagtttt tccgaccacg gcttgttcat cgcaattgcc
    36961 gcgttgaacc aggtatcgct ggtttcgttg ccggtaaaca gggtaatgaa gtttttcttc
    37021 ggcattttgc actgctcaac gcagagcttc atgatcagac gttcttgcgt acgaacgcgg
    37081 tccatcatga cgcgcatgct gttgaccagg tagtcaaact gcttcggcac caggcggaac
    37141 tgtttgaata cttcagacag tttcaggatc tcttcctgag cggcagcgtg actgcgacct
    37201 ttcgctttga tggtgtcacg cgttacaacg tactgagcgc gtagttccgc aaatttttcg
    37261 cgagccagtt ccgggtcgat gctgttgtca tcatcggcgc tgtcgtcatc gccatcttct
    37321 tcgtcttcat cttcgtcatc gtccagatct tcctgggaaa gctcagaacc gacgtgagtg
    37381 gcggtaggtg ccagatcttc ttctgcgttc gggtcaacaa agccggtgat cagatcggac
    37441 agacgcgctt cttctgcttc aacacgatcg tactgttcca gcagataggt gatcgcttcc
    37501 ggatattcag caacggagca ttgaacctgg ttgatcccgt cttcaatacg cttagcgatg
    37561 tcaatttcgc cttcgcgggt caacagttca acggtgccca tttcacgcat gtacatgcgt
    37621 accgggtcag tcgtgcgccc gatttcagat tccacgctgg aaagcacctg cgcggcggct
    37681 tcggcagcat cttcgtccgc ggtgttttca gccagcatca gatcatcggc atccggtgct
    37741 tcttccatca cctgaatgcc catgtcgttg atcatttgga tgatgtcttc gatctgatct
    37801 gaatcgacga tatcttccgg cagatggtca ttgacctcgg cataggtcag atagccttgc
    37861 tccttaccac gggtgacaag aagtttcagc tgtgactgcg ggttttgctc cataagacgg
    37921 tatccacact tatttcatga ggttggtgtt gggcgattga cccgatgtcc ttcagccgtt
    37981 aacgctgaaa tccaacggtt gtttaccgcc aacgataatt acgagggcgt acttatatat
    38041 ttgccgctgc ctctcagtgc ggctgtcggg ggcttcccga tcgctcttcg gcacttaagc
    38101 cgttaaatca ctttttcgcc agctcctggt ttaatgtcca gagctccagg cgttcttcgt
    38161 tgcttaaacc atgcgtgcgc tcacgagcga ttaactcttc ctggcgcagt tcaagcagcg
    38221 aatcaaacat atggttgagt gagtcggtga aggtttgctc agcaatattc ttatctgcta
    38281 tatcgtccca catcgacagt ttttcaaggg tggcagcatt atttgtacca cgatagtgct
    38341 ctaaaagttg cccggtggtc agacctggct gggagagaca agtgttgacc agttctctga
    38401 ataagccaag tccagggagc ttattttcat ccagattctc aagcggcggg accaacgtcg
    38461 ctaattctgg attttgcacc agcaacccta taagtatacg catggtcgtg cgttttagct
    38521 gcggaacagg gcgagaaacg ccgctctctg ccgcttttgg cattaatcgt tcaagctggc
    38581 tgtcatcaag tatgcctaat ttgttgccta attcctgacg aagatatatt cgcagcgttt
    38641 cgcccggcac ttgcgatatc aatggcagtg ccagcgtact caaacgtgcg cgcccgtcag
    38701 gggtactcag atcaacttgc ggcatcaggc tgttaaacag aaatgcggaa agcggcatcg
    38761 cctgctccat ccgcgcttca aacgcttctt taccttcttt tcgtaccagc gtgtcagggt
    38821 cttcgccatc cggcaaaaac ataaagcgta gctgacggcc gtctgtcatg taaggcagcg
    38881 ccgtttccag cgctcgccat gcggcatcgc ggcctgcacg gtcgccgtca taacagcaaa
    38941 tgacattgtt ggtcgcgcgg aacaacagtt gtatgtgatc ggcggtggtt gacgtaccta
    39001 acgacgcaac ggcgtaatta atgccgtatt gcgccagcgc caccacgtcc atatagcctt
    39061 cgaccacaag cagacgattg ggttcagcgt tatcctgctg cgcttcataa agaccgtaaa
    39121 gctggcggcc tttatggaaa atgtctgttt ctggcgagtt caggtatttg ggggtatcgt
    39181 tgcccagcac gcgcccgcca aaaccaatca cccgaccgcg tttatcgcga atggggaaca
    39241 tcacccgctc gcggaaacga tcgtaactgc gtccctgatc gttagtgacc aacatccccg
    39301 catcaatcaa tgactggcga ttttctggat tgccgccaaa ccgcttcagg acgttgtccc
    39361 agccgggggg cgcaaaacca atcgcaaagc gagcgataac ctcgtggctt aatccgcgtt
    39421 tttccagata ctggcgcgca gacgtggcaa caggttgttg taaagattgt tggtaaaacg
    39481 tattcagacc gtccatcaac tgataaagcg tttgcctctg atggcgctcg atctggctgg
    39541 ggccgctgcc tgcttcaaat ggcacttcaa gattgtgcat tgctgccagc tcttcgaccg
    39601 tttcaacgaa ctcgagcttg tcgtagttca tcaggaagtc gatcgcgttg ccgtgcgcgc
    39661 cacatccaaa gcagtggtaa aactgtttct caccgttaac ggtgaatgac ggggttttct
    39721 cgttgtggaa tggacaacac gcgtggaaat tcttgccctg ctttttcagc ttcacacggg
    39781 catcgatcag atcgacgatg tcagtgcgtg ccagcagatc attaatgaat acgcgtggga
    39841 ttcgtccagc cataggcccc gatttttagc aattcataaa cgaaaataag ccgcgcattc
    39901 ctttcggaag cacggcctta caactacaac tcggtctgat cggagagcaa cgctctcggg
    39961 gaattagtac agacgagtgc ggcgtgcgtt ttcgcgagcc agtttcttcg cgtgacgttt
    40021 cactgcagaa gctttagcgc gcttacgttc ggtagtcggt ttttcataga actcacgacg
    40081 acgaacttcc gccagaacac ctgctttttc gcaggaacgc ttgaagcgac gcagagctac
    40141 gtcgaacggc tcgttttcac gtactttaat taccggcatg tgcctctcac ctttgattaa
    40201 ttcggtttgc cgctggcatc aacgccagct tatttcaaaa tggtgcggaa ttttactgca
    40261 attgctgctg ctttgtaaag caccgcggcc tttttgaaag ggacttttat aagggtgagg
    40321 agtatacacg aggctttctc caggggcgaa caaagtttta catcaacccg cattggtcct
    40381 acactgcgcg gtaataaagc gaggtaaaac aagtcatgcg tgtactgggt attgaaactt
    40441 cctgcgatga aaccggcatc gccatttacg acgatgaaaa aggtttgtta gccaaccaat
    40501 tgtatagtca ggtgaaattg cacgctgact acggcggcgt cgtgcctgaa ctggcctccc
    40561 gcgatcacgt gcgcaaaacc gtaccgttga tccaggcggc gctaaaggag tctggcttaa
    40621 cggcaaaaga cattgatgct gtggcctata ccgcaggccc tggattagtc ggcgcactgc
    40681 tggttggcgc aaccgtgggg cgttctctgg cgtttgcctg gaacgttccg gcaatcccgg
    40741 tacaccatat ggaagggcat ctgttagcgc cgatgctgga agataacccg ccggaatttc
    40801 cgtttgtcgc gctgctggtg tccggcggtc atacgcagtt aatcagcgtg actggcattg
    40861 gtcagtacga gctgctcggc gagtctatcg atgatgccgc cggtgaagcg tttgataaaa
    40921 ccgcgaagct gctggggctg gattatcctg gtggaccgtt actgtcgaaa atggcggctc
    40981 agggtactgc cgggcgcttt gttttcccgc gtccgatgac cgaccgtccg gggctggatt
    41041 tcagcttctc cggtctgaaa accttcgcgg caaataccat tcgtgacaac ggcaccgacg
    41101 accagacgcg tgctgatatc gcccgcgcct ttgaagatgc ggtggtcgat acgctgatga
    41161 ttaagtgcaa gcgagcgttg gatcagactg gctttaagcg actggtcatg gcaggcggcg
    41221 tgagtgctaa ccgtacgtta cgggcgaagc tggctgaaat gatgaaaaaa cgccgcggcg
    41281 aagtgttcta cgcgcgtccg gagttttgta ctgataacgg cgcgatgatc gcctatgccg
    41341 gaatggtgcg gtttaaagca ggcgcgacgg cggatctcgg cgttagcgtg cgtccgcgct
    41401 ggccgctggc ggagttaccg gccgcgtaaa actatcgggt gccggagagc aatttccggc
    41461 accgtcctta cttaaagcaa caccacgggc atccacagta agccggtaat caccagcaat
    41521 acgaggaata tcagcccaaa aatcgccccc agtcgccagt aatccgccgt tggcagataa
    41581 ccactaccgt agtaaatcgg actgggtccg gtggcgtatg gtgtaagaat gctccccaga
    41641 ccaattgccg cgccaaccat caggcagaac accggcagcg ggatttccgg catcgccagc
    41701 gcagcggcaa tcatcatcgg tgcgagggcg gaggtatacg ccgtggcgct ggcgaaaaag
    41761 tagcgcagca gataaaacac cacaatcaac gccaccatca ccatcgttgg cgaataaccg
    41821 cttaagctgc ctgctaacag tttgccaaac cagctaataa aaccggtgtt gttgagtccg
    41881 gtagcgaggg tgataagcga ggccagccag aagaaaacgt tccacgccgc tttattactg
    41941 acaatgtcgt cccaactgat aatgcgcaga agcagcatca gtgccaccac gctgtaaccg
    42001 accatcgcgg catcgatata atcaccgccg aaaatccaca gcaccagcgc gcctaccatc
    42061 agccccagca tccgtttttc acgcgaacag agcgggccca ttgcctgcag ttccgtctct
    42121 gcccagcgcg gcacctgatc gccagacttc agtaccggcg ggtacagcac ataagccagc
    42181 cagggaacca gcagcaccag caaaatactg agcggcaaca tcccgaggaa ccagtcgccc
    42241 caactcagcg tggcgtgaga tgcgcttttc atcagtccaa ttaacagcaa gtttggtgcc
    42301 atcgccgtca agaaaatggc gctggtcacg cagtcggcaa caatccccat ccacatgatg
    42361 tacgagccaa tgctgcgcga actgctgtcg tttggttgtg attgatagag cggtggcagg
    42421 ttacggatga tgggatagat aatccccgca ccacgcgccg agttggacgg tgttacaggt
    42481 gcgaggatca gctcggagaa catcaccgca tagccgagaa acagcgtgcg atgtcccatc
    42541 tttttcacca gaaacagcgc aatgcggcgt ccaagcccgg ttttttcata gcctgtgcca
    42601 aacataaagg cggcgaaaat cagccagata accgaattag aaaaaccgga aaccgcccac
    42661 gagagggatt ttgcagtgaa tttaaagcct ggctgggcga gctgttcggg gctgaacagc
    42721 agccagggag agagtatggc gatgatggag atacccacca tcgccaccac ggcacccggc
    42781 acgggttcga ggatcagtcc aacgatcacg ccagtaaaaa cggcaaagta gagccaggta
    42841 tgactctcca gccctgcggg aaccggaatt agagcaataa tggcgatgac cgccagcggc
    42901 gcaaggtatc gccaccattc agtggaaggt ttcatcgtgt tatgcctgcg aaaaaggaga
    42961 gggggcgagc ctctccggga gggttatttg atgtaatgga cgtgctcgca gatctcttcc
    43021 acgatgggat cgcggcgttc ggcgaacagc tttttgtttt cggctatcag gttgttgccg
    43081 tgggtatcaa tagagacaat cagcgggccg aactctttga cccggcagac ccacagcgac
    43141 tccggcattc cgagctctgt ccagtgcact tcttcaatct cttccacctg ggttgccgcc
    43201 agcaccgcgc agcctgcagg gaaaatcaca tgtagcgcct tgaatttctg acagccttct
    43261 tcggtcagcg gccccattcc gcctttgccg accaccattt tcacgccggt ctgctcaata
    43321 aattcacgtt caaaactttc catacgcata ctggttgtcg ggccgacgga gaccatctcc
    43381 catttgtcgc cgtttttgcg cacgatgggg ccagcgtgga aaatcgcttt gccgttgaga
    43441 tcgtaaggga tcggacgttt cagttcgatc aaacggcggt gacaaacgtc gcggcaggtc
    43501 accagcgtac cggtcaggta gatcacatcg ccgacgcgaa tatcttgcag atcttcagct
    43561 ttgatcgggg ttgtcaggat ctttttcata acgcgctccg ggtgtgagac agattttcaa
    43621 aggtgagatc ggcatgaacc agcagcgtgc cgcgacgatg cgcccagcag ccggtagaga
    43681 cagcaacgcc gatggttgac ggatggcggg cggcagattc gatatgcacg cccatcactg
    43741 aactgttgcc ggtcagccct tgtggaccaa tccccagacg gttgagtcct tcttccaggc
    43801 gtagctccag ctctgccgct tttggattgg gatggcggct gccaatcgga cgcaaaatgg
    43861 ctttacgcga gagtacggcg gcggtttcca ccgaggtggc gatgcccacg cccaccagta
    43921 ccggtggaca ggcgtttacg gcgagggtgg agatattttc gaagacgaat ttcaccacgc
    43981 cttcgtagcc ttctgacggc attaacactt tcgagcggcc aggtagcgtg cagccgccgc
    44041 ctgccatgta aacttcgatt tccgcatcgt cattgtcggg gatgatgtcc caggtgaccc
    44101 acggtacgcc gctaccggta tttttgccgg tgtttacttc gtcaaaaatt tctaccgcat
    44161 tgtgacgtag tggcgctttg acggttgcct cttccacggc ttgtttgagt atgctttgca
    44221 gctcgccaag cagtgggaag cgggaaccga ctttaacgaa gaacataatc tccccggtgt
    44281 cctgacaggc aggacgattc aggtcaatcg ctttttgcat gttgtcgaac atcgtatggt
    44341 agataatttt ccccatcgac gacgtttcgg catcctttag ctgttttagt ttatccacca
    44401 cgtcatcagg cattcgggta gaaatcatgg cggtaaagtt agcgacaatc tctgtcaact
    44461 tattcactgc ctgttgctta ttactttcgc tcatcatggc aactccagtt tttgtatttg
    44521 tctggaaaaa ttcaccgcca caaactacca ctaaaaatgt ggttttatat tttttgccac
    44581 cgtgaatatg taattaacag ttcgtgaatc aacgcgaaaa taagaatgaa tattgcgcca
    44641 gagcagggaa tggcaatgtt ttgcgaaata cactgcgctt atcacaactg cggataatta
    44701 acgatgctga atagctggcc tttagccaaa gacttgcagg tactggtaga aatcgtccac
    44761 agcgggagtt ttagtgctgc agcggcgacg cttgggcaga cgcctgcatt tgtcaccaag
    44821 cggatccaaa tccttgaaaa tacgctggca acaacgctgc tgaaccgctc cgctcgcggc
    44881 gtggcgctga ctgaaagtgg ccaacgttgt tacgaacatg cgctggaaat cctcacccaa
    44941 tatcagcgac tggtcgatga cgtcacgcaa atcaaaacgc gcccggaggg gatgattcgt
    45001 attggttgca gttttggttt tgggcgcagc catattgcgc cagccattac cgaacttatg
    45061 cgcaattatc ctgagttaca ggtgcatttt gaattgttcg atcggcaaat tgatttagtg
    45121 caggataata ttgatctgga tattcgtatt aatgacgaaa ttcctgatta ttatattgcg
    45181 catctgttaa cgaaaaataa aagaatatta tgcgcagcac ctgagtatct gcaaaaatat
    45241 ccgcagccac aatcattaca ggaattaagt cgtcatgact gtctggtgac caaagagcgc
    45301 gatatgaccc acggaatatg ggagttgggg aacggtcagg agaaaaaatc ggtgaaggtt
    45361 agcgggcatc tttcctccaa tagcggcgag atagtgttgc aatgggcgct ggagggcaaa
    45421 gggattatgc tgcgttccga gtgggatgtg ttgccgtttc tggagagtgg caaactggtg
    45481 cgagtattgc cggagtatgc acaaagcgcc aatatctggg ctgtttaccg ggagccgctc
    45541 tatcgcagca tgaaattacg tgtctgcgtg gaatttctgg cggcatggtg ccagcaacgg
    45601 ctgggcaagc ccgatgaagg ctatcaggtc atgtagatcc accagaaatc actcgggatc
    45661 cttttcgcgc tttcttttga atttcgtcca gatttttgtc tcctgacgac gccacagacg
    45721 ctggatgttg tcatgatgac gcagcaggat caggcaagag agcatcgaaa ccgggaaggt
    45781 gaattgtggc ttaaaccacc agacataaaa tggagcaatc agtgcactga caatcgctcc
    45841 cagcgacgag tatccgctca atagcacggt cagtaaccag gttcccgcca ttactccggt
    45901 gagatcccag ccaatgggtg cgatggcacc aaaagcggta gcaacgcctt ttcctccttt
    45961 aaatccgaag aaaacgggcc agatgtgtcc aagacaggcg gcgatggcaa ttaagcctag
    46021 ccagaagggg ctgacaccta attcatacgc gccccagacg ggcaacattc ctttcagaac
    46081 gtcgaaaatc agtactgcta cggctgctcc cttgccaccg atacgtaaca cattggttgc
    46141 gcctggattg ccggagccgc tggttcgcgg atcgggcagc ccacacaagc ggcaaaccag
    46201 aatggcactg gaaatggagc cgcagaggta cgcgatgagg atcattccag gcgcgattgc
    46261 actcataagc tgttccgttt tgaaaattcg tgttcaacga tgaatctgtg gataatacgc
    46321 acatttcgcc ggaagtggta tccggttagc caaaaagcag gcaggacgtg atggatattg
    46381 tatttataga gcaactttcg gtaatcacca ctattggtgt ttacgactgg gaacagacca
    46441 tcgaacagaa gttagtgttc gatatcgaaa tggcatggga taaccgtaaa gcggcgaaaa
    46501 gtgatgatgt ggcggattgc ctcagttacg ctgacattgc agaaacggtg gtcagccacg
    46561 tcgagggggc gcgttttgcg ctggtggaac gcgtggctga agaggtggcg gagctgctgt
    46621 tagcacgctt caactcgccg tgggtgcgta tcaaactcag caagccaggc gcagtggcgc
    46681 gggcggcgaa tgttggcgta atcattgagc gtggcaataa tctgaaagaa aataattaat
    46741 tttacagctg ttaaaccaaa cggttataac ctggtcttac tgcagtagtc cggacaagcg
    46801 gtacatttta ataatttagg ggtttattga tgagcgatat gcactcgctg ctgatagcgg
    46861 caatattggg tgtggtcgaa ggattgacag aatttctgcc ggtatccagc acgggccata
    46921 tgattattgt cggtcacttg ttggggttcg agggcgacac ggcgaaaacc tttgaagttg
    46981 tgatccagtt aggatcaatt ctggcggtag tagtgatgtt ctggcggcgt ctgtttggcc
    47041 tgattggcat ccactttggc cgcccgttgc agcacgaagg agaaagcaaa ggtcgtttaa
    47101 cgctgatcca cattttgctg gggatgattc cggcggtggt actggggctg ttgttccacg
    47161 acacgattaa gtcattgttt aacccgataa atgtgatgta tgcactggtc gttggcggtt
    47221 tgttgctgat tgccgccgaa tgcctgaagc cgaaagagcc gcgtgcgccg ggtcttgatg
    47281 atatgaccta tcgtcaggcg tttatgattg gctgtttcca gtgtctggcg ctgtggccgg
    47341 gtttctcccg ttccggggcc accatttcag gtgggatgct gatgggcgtg agccgttacg
    47401 ctgcttccga gttctcgttc ctgctggcgg taccgatgat gatgggcgca acggcgctcg
    47461 atctctacaa aagctggggc ttcctgacaa ccggcgatat cccgatgttt gccgttgggt
    47521 ttatcaccgc ttttgtggtg gcgctgatag cgattaaaac cttcctgcaa ttgattaagc
    47581 gcatttcgtt tatcccgttc gccatttatc gctttattgt ggcggctgcg gtgtacgtcg
    47641 tgttcttttg attgagaaaa ggttcgggag caggcctggt tttctccctc gctctgtagg
    47701 gagagggccg gggtgggggt gattgttcgc accggtgctg gtctgttccc ctcaccctaa
    47761 ccctctcccc gaaggggcga ggggatggtt cgtgttaacg tttagtttta tactcagtca
    47821 ggctttgggc aacgttgttc cttccaactg gctaccgccg caatccgccg tcgggtcagc
    47881 tcctcgcgaa tctccacacc tttaaatccc gcttcaacga ccgcttttgt cggcactgac
    47941 tgcgccactt cccaggcttc gcgcaaccaa cggccttgcg ggtagtccgc tgattcaaaa
    48001 ccggttctgc cgcgtacgtc agcctcgctg gtcagcgcca gttgctcgac acgctgcggt
    48061 ttacgccagg catcgatgga atcaaataat ttgacgatgg tttttgggtt cagcattggg
    48121 aaggtgtgga tgagatcgtg aaactcagcc accagtctgg ctaaatcgcg aatttcattt
    48181 ggcacgcgca gacgctggca tagttgttcc actaacttaa cacccgccgg gccatgccca
    48241 tgatgacgcg gccaaagttc tggcggcgtc agtcctttac cgagatcgtg gcataaagtt
    48301 gcgaaacgga catcaacctg cggactcagc atcgccgcca ttgagagcgt cattaaggta
    48361 tgaatacccg tgtcgatttc cggatgccac ctggcagggg ccggaacgcc aaacagggcg
    48421 tcaatttccg ggaataaaac gcgaagtgca ccgcaatcgc gcagtacctg gaagaacacc
    48481 tggggattgc gggtggtgag ggcgctttcc gtctctttcc atacccgttc aggcgtcagg
    48541 tgttccagtt cacccgcatg ggtcatctcg cgcatcagcg tcagagtttc atcggcaata
    48601 cgaaaaccga ggtgggcata acgcgcagca aaacgcgcca cgcgcagaac gcgtaacgga
    48661 tcttcgccaa aagcggggga aacatggcgc aacagacgat tttgcagatc gcccagaccg
    48721 ttgtacgggt cgataatctc gccgttatcg tcctgagcca gcgcattaat ggtcagatcg
    48781 cgacgcttaa gatcatcttc cagcgtgaca tccggtgcgg catagcaagt aaaaccggtg
    48841 taaccggaac cggatttccg ttcggtacgt gccagcgcat actcttcatg cgtttgcgga
    48901 tgcagaaaca caggaaaatc gcggcctacc tgctggtagc ccgcgtcgag catctcctgt
    48961 ggcgtactgc cgaccaccac ccaatctctg tctttgaccg gtagccctaa caatgcatcc
    49021 cgaacagcac caccgaccag ataaatcttc acgccatcat cccttcgtca aatacacaat
    49081 tcctaataat aagacagtgc aacgaagaag gcgatttagt tcatccagcg atctttgcgt
    49141 ttgcgcgttg ggatcaggtg tggcagtacc agaccgagca gcaagccaag ccccagcacg
    49201 ccaccgccat acataaacca ttgcatgatg atggtgcgct gtttgtcatc cagctgtacg
    49261 ctggcggcat cgaccttttt ctgcgcgaca atcagctcgt ttttcaattt ctgattttct
    49321 tcttttaatc cgttgatcac gctgtcgctc tgcgccactt tttgctgcat ttctgcggtg
    49381 cgctgattcc aggtgttatc gatattggtg agtttatcgg tcagggtttt gacctgattt
    49441 tccagatctg gcacacggga gcgcaggctt ggctcagtgc taagttgttt caacgggatc
    49501 caggcggtac ggccagagct gtctttcacc tgggcataat tggtgttggc gtcagtttgt
    49561 aataaggtca cttcctcgcc ggcgttaacc gtgcccacga ggcgataatg atctcccgga
    49621 ccgctacgga cccaggtatt cagttcatca gaaacatagc gcgtttcttc agcgtgtgag
    49681 acggcagtgg cgctaagtgc gagtaaagtt aatccgatca ggcgtaattt tggcatcagg
    49741 ctgtcgttat tgtcatggaa agtggaacga tagtagtggc atcagtgtcg ctacgcaaag
    49801 cattcgacat caatcggaat cctctgtgtc aggtcttgcc actgcttcaa acttttacgc
    49861 ccgtcaaatt gcccgttgca tggcaatttg gcgcaaaata ctatctactg acaaaaaaga
    49921 tcgcattaag ttcgccgttc atcgtcactt atgtgacata accgataagt aaaggccatg
    49981 gctcaggaaa tcgaattaaa gtttattgtt aatcacagtg ccgttgaggc gttgcgtgac
    50041 catctcaata cgctgggcgg cgagcaccat gaccccgtgc agttgctgaa tatttactac
    50101 gaaacgccgg ataactggct gcgtgggcac gatatgggct tacgtattcg tggcgaaaac
    50161 ggtcgctatg agatgaccat gaaagttgca ggaagagtga caggcggctt acaccagcgc
    50221 ccggaatata acgtggcgtt gagcgaaccg acgctcgacc tggcgcagtt accgacggaa
    50281 gtctggccga acggcgaatt gcccgccgat ctcgcttccc gcgtacaacc gctgttcagc
    50341 accgattttt atcgcgaaaa atggctggtg gcggtcgatg acagccgaat tgaaatcgct
    50401 ctcgatcagg gggaggtgaa agcgggggaa tttgctgaac ctatctgtga gctggaactg
    50461 gaactgctta gcggcgacac gcgcgcggtg ctgaaactgg cgaaccaact ggtatcgcaa
    50521 accggattac gccagggcag cctgagcaaa gcggcgcgtg gctatcatct ggcgcagggc
    50581 aatccggcgc gtgaaatcaa accgaccacc attttgcatg ttgcggcaaa agccgatgtg
    50641 gaacaggggc tggaagcggc gctcgagctg gcgttagcgc aatggcagta tcatgaagaa
    50701 ctgtgggtac gcggcaacga tgcggcgaaa gaacaggtgc tggcagccat tggtctggtc
    50761 cgtcatacac tgatgctgtt cggtggtatt gtgccgcgca aagcgagcac tcacttacgt
    50821 gatttgctga ctcaatgcga agcgaccatc gcttctgcgg tttctgccgt gacggcggtc
    50881 tactctacca aaacggcaat ggcgaagctg gcgttgaccg aatggctggt aagcaaagcc
    50941 tggcagccgt ttttagatgc caaagcgcag agtaaaatga gcgattcctt caaacgcttt
    51001 gccgatatcc atctttcccg ccatgccgct gaactgaaaa gcgttttctg ccagccgttg
    51061 ggcgatcgct accgtgacca gttgccacgt ctgacgcgtg atattgactc aatactgttg
    51121 ctggcgggtt actatgatcc tgtcgtcgcg caagcctggc tggagaactg gcaggggctg
    51181 cgtcacgcta ttgcgaccgg gcaacgcatt gaaattgaac atttccgtaa tgaggcaaac
    51241 aatcaggaac cgttctggtt gcacagcgga aaacgttaat caggcaagga taaaatcgct
    51301 tatgaagccg ctctcttcac cgttacagca gtactggcag accgttgttg agcggctgcc
    51361 agagccttta gccgagaaat cacttagcgc acaggcgaag tcagtactta ctttcagtga
    51421 ttttgtgcag gacagcgtga ttgcgcatcc agagtggctg acggaactgg aaagccaatc
    51481 gccgcaggcc gacgaatggc agcattacgc ggcatggttg caggaggcgc tcagcaatgt
    51541 gagtgacgaa gccgggttaa tgcgcgagct gcggctattc cggcggcgca ttatggtgcg
    51601 catcgcctgg gcgcaaacgc tggctttggt tactgaagag agcatattgc agcagctcag
    51661 ccatctggcg gagacgctga ttgtcgccgc gcgtgactgg ctgtatgacg cttgctgccg
    51721 cgagtgggga acgccgtgca atgcgcaggg cgaagcgcaa ccgctgctga ttttaggcat
    51781 gggtaagctg ggcggtgggg aactgaattt ctcctcagat atcgatctga tttttgcctg
    51841 gccggaacat ggttgtacgc agggcggacg ccgggaactg gataacgcgc agttttttac
    51901 ccgcatgggg cagcggctga ttaaagtgtt ggatcaacca acgcaggatg gcttcgtcta
    51961 tcgcgtggat atgcggctgc gtccgtttgg cgaaagtggc ccgctggtgc tgagctttgc
    52021 cgcgttggag gattattacc aggagcaggg gcgcgactgg gagcgttacg cgatggtcaa
    52081 ggcgcggatt atgggcgata gcgaaggcgt ctatgctaac gagttgcgtg cgatgctgcg
    52141 cccgtttgtt ttccgtcgtt acatcgattt cagcgtgatt cagtcgctgc gcaacatgaa
    52201 agggatgatt gcccgtgaag tgcgtcgacg tggtttgacc gacaatatca aactcggcgc
    52261 aggcggcatt cgcgaaattg aatttatcgt tcaggtgttc cagctcattc gcggtgggcg
    52321 cgaaccgtcg ctgcagtcgc gctctttact gccaacgctc agcgtcattg ccgcgctgca
    52381 tctgctttct gaaaacgatg ctgaacaatt gcgagtggcg tatctgttcc tgcggcgtct
    52441 ggaaaacctg ctgcaaagca ttaacgacga acaaacccag acgcttcctt ctgatgagct
    52501 taaccgtgcg cggctggcgt gggcgatgga ctttgctgac tggccgcaac tgaccggggc
    52561 gctgaccgca catatgacca atgtgcgccg ggtgtttaat gaattgattg gcgacgacga
    52621 aagtgaaact caggaagagt cgctgtcgga acagtggcgt gagctgtggc aggatgcgtt
    52681 gcaggaagat gacactacgc cagtgctggc gcatcttagc gaggatgatc gcaaacaggt
    52741 gctaacgctg attgccgatt tccgcaaaga gctggataag cgcaccatcg ggccgcgagg
    52801 acgtcaggtg ctcgaccatc tgatgccgca tctgctaagt gatgtctgtg cgcgtgaaga
    52861 cgctgccgtt acgctgtcgc gcattaccgc cttgctggtg gggattgtta cccgcaccac
    52921 ctatttagaa ttgctcagtg aattccccgc ggcgcttaaa catttgattt ctctgtgtgc
    52981 cgcgtcgccg atgattgcca gccaactggc gcgttatcca ttattgctgg atgaattgct
    53041 cgatccaaac accctttacc agccgacggc gaccgatgcc taccgcgatg agttgcgcca
    53101 gtatttgctg cgcgtgccgg aagatgacga agagcaacag cttgaggcgc tgcgtcagtt
    53161 caaacaggcg cagctgttac gcatcgccgc agcggatatc gctggtacgc taccggtgat
    53221 gaaagtgagc gatcacttaa cctggctggc agaggctatg atagatgccg tcgttcagca
    53281 ggcgtgggtt caaatggtgg cccgctacgg taagccaaat cacctcaacg atcgcgaagg
    53341 ccgtggtttt gcggtggtcg gctacggcaa gctgggcggc tgggagttag gctacagttc
    53401 cgatcttgac ctgatcttcc tccatgattg cccaatggat gcgatgactg acggcgagcg
    53461 ggaaatcgac gggcgacagt tttatctgcg tctggcgcaa cgcattatgc atctgttcag
    53521 tacgcgtacc tcttccggca ttttgtatga agtggatgca cggctacgcc cgtccggggc
    53581 cgcgggaatg ctggtgacat ccgcagaagc atttgccgat tatcagaaaa acgaggcctg
    53641 gacgtgggaa catcaggcgc tggtgcgtgc gcgggtagtg tacggcgatc cgcagctcac
    53701 cgcgcacttt gacgcggtgc gtcgcgagat tatgacgctg ccgcgtgaag gtaaaactct
    53761 gcaaacggaa gtgcgggaaa tgcgcgagaa aatgcgcgct catctcggca ataaacatcg
    53821 cgatcgcttt gatatcaaag ctgatgaagg cggaattacc gatatcgaat ttattaccca
    53881 atatctggtg ttgcgctacg ctcatgaaaa accgaagtta acgcgctggt cagacaacgt
    53941 gcgcattctg gaactactgg cgcaaaacga cattatggaa gagcaggaag cgatggcgct
    54001 gacccgtgct tatactacgc tgcgcgatga acttcatcat ctggcattac aggaattgcc
    54061 gggccatgtg ccggaggatt gcttcaccgc agagcgtgaa ctggtgcggg caagctggca
    54121 gaagtggctg gtggaagaat gaagtatggt attatcgcgc gcaaattttg aatctctcag
    54181 gagacaggaa tgaaagtaac gctgccagag tttgaacgtg caggagtgat ggtggttggt
    54241 gatgtgatgc tggatcgtta ctggtacggt cccaccagcc gtatctcgcc ggaagcgccg
    54301 gtgcccgtgg ttaaagtcaa taccatcgaa gaacgtccgg gcggcgcggc taacgtggca
    54361 atgaacatcg cttctctcgg tgccaatgct cgcctggtcg ggctgacggg cattgacgat
    54421 gcggcgcgtg cgctgagtaa atctctggcc gacgtcaacg tcaaatgcga cttcgtttct
    54481 gtaccgacgc atccgactat caccaaatta cgggtacttt cccgcaacca acagctgatc
    54541 cgcctggatt ttgaagaagg tttcgaaggt gttgatccgc agccgctgca cgagcggatt
    54601 aatcaggcgc tgagttcgat tggcgcgctg gtgctttctg actacgccaa aggtgcgctg
    54661 gcaagcgtac agcagatgat ccaactggcg cgtaaagcgg gcgttccggt gctgattgat
    54721 ccaaaaggta ccgattttga gcgctaccgc ggcgctacgc tgttaacgcc gaatctctcg
    54781 gaatttgaag ctgttgtcgg taaatgtaag accgaagaag agattgttga gcgcggcatg
    54841 aaactgattg ccgattacga actctcggct ctgttagtga cccgttccga acagggtatg
    54901 tcgctgctgc aaccgggtaa agcgccgctg catatgccaa cccaggcgca ggaagtgtat
    54961 gacgttaccg gtgcgggcga cacggtgatt ggcgtcctgg cggcaacgct ggcagcgggt
    55021 aattcgctgg aagaagcctg cttctttgcc aatgcggcgg ctggcgtggt ggtcggcaaa
    55081 ctgggaacct ccacggtttc gccgatcgag ctggaaaatg cagtgcgtgg gcgcgcagat
    55141 accggctttg gtgtgatgac cgaagaggaa ctgaagcttg ctgtagcggc agcgcgtaaa
    55201 cgcggcgaaa aggtggtaat gaccaacggt gtctttgaca tcctgcacgc cgggcacgtc
    55261 tcctatctgg caaatgctcg caaactgggt gaccgtctga ttgtcgctgt gaacagcgat
    55321 gcctccacca aacggctgaa aggggattcc cgccccgtta acccgctcga acagcgtatg
    55381 attgtgctgg gcgcactgga agcggtcgac tgggtggtgt cgtttgaaga agacacgccg
    55441 cagcgcttga tcgccgggat cctgccagat ttactggtga agggcggcga ctataaacca
    55501 gaagagatcg ccgggagtaa agaagtctgg gccaacggcg gcgaagtgct ggtgctcaac
    55561 ttcgaagacg gttgctcgac cactaacatc atcaagaaga tccaacagga taaaaaaggc
    55621 taaccggaaa gcggttcaca gatctcgctt cgtagcaggc aggcctattg acgctgttgt
    55681 tttttcaacc taaagtaaag gaacaagggt aagggaggat ttctcccccc tctgatcagt
    55741 tgttagtaag tcgggaaact taacagtaac aacacaacca gtatgatgac gaacttcatc
    55801 ataacccttt ccttctataa ggccccttcc tcgggagggg ctttcccgtt tcagcccctt
    55861 gctgactccc cgattgttga tgagcgccgg accccgcgca acgctttccc tgaagcagtt
    55921 ttgccttccc cgagcggtca cgcaaaacgc tgcaacgtcc tgagcatttg caccttttag
    55981 cggaacgcag atataaaaaa tccccggctt tttgggccag ggatatgtgt taatgaaacc
    56041 ttcttgacgc tgattttttt tcaacctaaa gtaaaggaac aagggtgagg gaggatttct
    56101 cccccctctg attggctgtt aataagctgc gaaacttacg attaacaaca caatcagtat
    56161 gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc ccttcctcgg gaggggcttt
    56221 cccgtttcag ccctttgctg actccccgat tgttgatgag cgccggacct cgcgcaacgc
    56281 tttcccagag gcagttttgc cttccccgag cggtcacgca aaacgttgca acgacctgat
    56341 catttgcagt ttttagcgga acgcagatat aaaaaatccc cggctttttg tgccagggac
    56401 gttcatagct gaaaatttat tctacgtttt cggcgacggg agttgttgag gttaagggtg
    56461 aagtcaatgc cgccagtgag ccgcctgaaa cgccaatttc attgagcaag gagtcaatca
    56521 gcggtgcctg tgtgcggtaa gagagagcgg ctgataatgc ttgttccgcc aggtttccgc
    56581 cccccacatt acccgtgttt gcatcacccg cagcgctgcc acgattcaga ccatcgacct
    56641 gaataatctt gataccgtca atagatttca tcggctcaac ggatttttct attaccgcag
    56701 gtagcgcctg caacaaggcc agtttgaatt taaggctggt ttgttcatca gaaagtacgt
    56761 tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc cagacccttt ttacgtgtag
    56821 cttcggctaa ctcaacgata gccgccgcct gcatctctgc ggcttctttt tctgctttcg
    56881 cccgcacggt cagttcaacc gctttggttt ctgcatcctg cgcggcagcg attagggcaa
    56941 cttgtttagc acgatcggct tctgcggtct ggcgagtggt ttctacgttt tgttgcgcac
    57001 ttactgcttc tgcgagtgca aggttagcac gcgcttctgc ctgggactgt tgttccgatt
    57061 tggcggcaat agcgatcgat ttcgtctggt tggcgatttc ggtgacctgc tgttgttcga
    57121 tctctttaat acgaacttca cgttcagcct caacctttct tgagcggacg gcctgctcgc
    57181 gctcgatctc cgtttcctga atttgtcgtt cagccagaat tcgcgtctgc tctgcttcac
    57241 gacgacgttc agcttcaaaa gccgcaatac gtgcattctg ttcggcagta cgggttttaa
    57301 cctgttgctc ctgctcaagc gtcataaacg cttcttgttg ttcaatctcc aacttgcgcg
    57361 aaagtgcatc acggtttttc tcacgcaccg caacttctac atcctgttca acttcgttac
    57421 gttcgcgacg acggcgctcc gtctcctgag tcagtttggt taaaccttcg gcgtcaaagg
    57481 cattgttcgg attgaaatgt tctttcgagg tctggttaaa gttggtaagt gaaacgcttt
    57541 ccagctcaag accgtttttc gacaggtctt ctgcgacagt attttgtact ccctgcacga
    57601 agttctcgcg ggtatcctgt aactcatgca tggtcatttg tgcagctgtt gcacggaggg
    57661 catcgacaaa tttatcttca accaacatac gtaagtcttc tggtgatagc gtgcgttgcc
    57721 ccaacgtctg cgccgcagtg gcgatgcctt ctacagacgg ttttacccgt acaaagaaag
    57781 cgactactac atcaacacgc atacgatctt tcgtaatcag gctatcaatg gttgagcggc
    57841 tgacttccag cttcagagta ttcatattga tggggattat ttcatgaaag atcggcatca
    57901 cgattgcgcc accgctcatc accacttttt gcccacctaa accagtacga acaaaagctt
    57961 gctctgccga agcgcgacga tagagcctgg cgaaaataat tccaataata aacagaatgc
    58021 atacggcaat aatcgcggta aacatccagg agggcacaga
//
LOCUS       DAPHDX010000008        57913 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPHDX010000008 DAPHDX010000000
VERSION     DAPHDX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.008"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..459)
                     /gene="folK"
                     /locus_tag="RH058_000505"
     CDS             complement(<1..459)
                     /gene="folK"
                     /locus_tag="RH058_000505"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV3136144.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAF"
     gene            complement(456..1874)
                     /gene="pcnB"
                     /locus_tag="RH058_000506"
     CDS             complement(456..1874)
                     /gene="pcnB"
                     /locus_tag="RH058_000506"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV3136145.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1913..2839)
                     /gene="gluQRS"
                     /locus_tag="RH058_000507"
     CDS             complement(1913..2839)
                     /gene="gluQRS"
                     /locus_tag="RH058_000507"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV3136146.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2876..3331)
                     /gene="dksA"
                     /locus_tag="RH058_000508"
     CDS             complement(2876..3331)
                     /gene="dksA"
                     /locus_tag="RH058_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV3136147.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3509..4213)
                     /gene="sfsA"
                     /locus_tag="RH058_000509"
     CDS             complement(3509..4213)
                     /gene="sfsA"
                     /locus_tag="RH058_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV3136148.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4228..4758)
                     /gene="thpR"
                     /locus_tag="RH058_000510"
     CDS             complement(4228..4758)
                     /gene="thpR"
                     /locus_tag="RH058_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV3136149.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDAREAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4832..7261
                     /gene="hrpB"
                     /locus_tag="RH058_000511"
     CDS             4832..7261
                     /gene="hrpB"
                     /locus_tag="RH058_000511"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV3136150.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7457..9991
                     /gene="mrcB"
                     /locus_tag="RH058_000512"
     CDS             7457..9991
                     /gene="mrcB"
                     /locus_tag="RH058_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV3136151.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRSKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10211..12454
                     /gene="fhuA"
                     /locus_tag="RH058_000513"
     CDS             10211..12454
                     /gene="fhuA"
                     /locus_tag="RH058_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV3136152.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12505..13302
                     /gene="fhuC"
                     /locus_tag="RH058_000514"
     CDS             12505..13302
                     /gene="fhuC"
                     /locus_tag="RH058_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV3136153.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13302..14192
                     /gene="fhuD"
                     /locus_tag="RH058_000515"
     CDS             13302..14192
                     /gene="fhuD"
                     /locus_tag="RH058_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV3136154.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14189..16171
                     /gene="fhuB"
                     /locus_tag="RH058_000516"
     CDS             14189..16171
                     /gene="fhuB"
                     /locus_tag="RH058_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV3136155.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16206..17486)
                     /gene="hemL"
                     /locus_tag="RH058_000517"
     CDS             complement(16206..17486)
                     /gene="hemL"
                     /locus_tag="RH058_000517"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV3136156.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAKEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17711..19132
                     /gene="clcA"
                     /locus_tag="RH058_000518"
     CDS             17711..19132
                     /gene="clcA"
                     /locus_tag="RH058_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV3136157.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19156..19221
                     /locus_tag="RH058_000519"
     CDS             19156..19221
                     /locus_tag="RH058_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136158.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19214..19558
                     /gene="erpA"
                     /locus_tag="RH058_000520"
     CDS             19214..19558
                     /gene="erpA"
                     /locus_tag="RH058_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV3136159.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19605..20228)
                     /locus_tag="RH058_000521"
     CDS             complement(19605..20228)
                     /locus_tag="RH058_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV3136160.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20266..21066)
                     /gene="btuF"
                     /locus_tag="RH058_000522"
     CDS             complement(20266..21066)
                     /gene="btuF"
                     /locus_tag="RH058_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV3136161.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21059..21757)
                     /gene="mtnN"
                     /locus_tag="RH058_000523"
     CDS             complement(21059..21757)
                     /gene="mtnN"
                     /locus_tag="RH058_000523"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV3136162.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21841..23358
                     /gene="dgt"
                     /locus_tag="RH058_000524"
     CDS             21841..23358
                     /gene="dgt"
                     /locus_tag="RH058_000524"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV3136163.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23488..24912
                     /gene="degP"
                     /locus_tag="RH058_000525"
     CDS             23488..24912
                     /gene="degP"
                     /locus_tag="RH058_000525"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV3136164.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25067..26224
                     /gene="cdaR"
                     /locus_tag="RH058_000526"
     CDS             25067..26224
                     /gene="cdaR"
                     /locus_tag="RH058_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV3136165.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26313..26699)
                     /locus_tag="RH058_000527"
     CDS             complement(26313..26699)
                     /locus_tag="RH058_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV3136166.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27014..27838)
                     /gene="dapD"
                     /locus_tag="RH058_000528"
     CDS             complement(27014..27838)
                     /gene="dapD"
                     /locus_tag="RH058_000528"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV3136167.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27869..30541)
                     /gene="glnD"
                     /locus_tag="RH058_000529"
     CDS             complement(27869..30541)
                     /gene="glnD"
                     /locus_tag="RH058_000529"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV3136168.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30603..31397)
                     /gene="map"
                     /locus_tag="RH058_000530"
     CDS             complement(30603..31397)
                     /gene="map"
                     /locus_tag="RH058_000530"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV3136169.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31765..32490
                     /gene="rpsB"
                     /locus_tag="RH058_000531"
     CDS             31765..32490
                     /gene="rpsB"
                     /locus_tag="RH058_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV3136170.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32748..33599
                     /gene="tsf"
                     /locus_tag="RH058_000532"
     CDS             32748..33599
                     /gene="tsf"
                     /locus_tag="RH058_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV3136171.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33746..34471
                     /gene="pyrH"
                     /locus_tag="RH058_000533"
     CDS             33746..34471
                     /gene="pyrH"
                     /locus_tag="RH058_000533"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV3136172.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34763..35320
                     /gene="frr"
                     /locus_tag="RH058_000534"
     CDS             34763..35320
                     /gene="frr"
                     /locus_tag="RH058_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV3136173.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35412..36608
                     /gene="ispC"
                     /locus_tag="RH058_000535"
     CDS             35412..36608
                     /gene="ispC"
                     /locus_tag="RH058_000535"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV3136174.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36797..37555
                     /gene="ispU"
                     /locus_tag="RH058_000536"
                     /gene_synonym="uppS"
     CDS             36797..37555
                     /gene="ispU"
                     /locus_tag="RH058_000536"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV3136175.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37568..38425
                     /gene="cdsA"
                     /locus_tag="RH058_000537"
     CDS             37568..38425
                     /gene="cdsA"
                     /locus_tag="RH058_000537"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV3136176.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38437..39789
                     /gene="rseP"
                     /locus_tag="RH058_000538"
     CDS             38437..39789
                     /gene="rseP"
                     /locus_tag="RH058_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV3136177.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39819..42251
                     /gene="bamA"
                     /locus_tag="RH058_000539"
     CDS             39819..42251
                     /gene="bamA"
                     /locus_tag="RH058_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV3136178.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42373..42858
                     /gene="skp"
                     /locus_tag="RH058_000540"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42373..42858
                     /gene="skp"
                     /locus_tag="RH058_000540"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV3136179.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42862..43887
                     /gene="lpxD"
                     /locus_tag="RH058_000541"
     CDS             42862..43887
                     /gene="lpxD"
                     /locus_tag="RH058_000541"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV3136180.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43992..44447
                     /gene="fabZ"
                     /locus_tag="RH058_000542"
     CDS             43992..44447
                     /gene="fabZ"
                     /locus_tag="RH058_000542"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV3136181.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44451..45239
                     /gene="lpxA"
                     /locus_tag="RH058_000543"
     CDS             44451..45239
                     /gene="lpxA"
                     /locus_tag="RH058_000543"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV3136182.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45239..46387
                     /gene="lpxB"
                     /locus_tag="RH058_000544"
     CDS             45239..46387
                     /gene="lpxB"
                     /locus_tag="RH058_000544"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV3136183.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46384..46980
                     /gene="rnhB"
                     /locus_tag="RH058_000545"
     CDS             46384..46980
                     /gene="rnhB"
                     /locus_tag="RH058_000545"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV3136184.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47017..50499
                     /gene="dnaE"
                     /locus_tag="RH058_000546"
     CDS             47017..50499
                     /gene="dnaE"
                     /locus_tag="RH058_000546"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV3136185.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50512..51471
                     /gene="accA"
                     /locus_tag="RH058_000547"
     CDS             50512..51471
                     /gene="accA"
                     /locus_tag="RH058_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV3136186.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51570..53711
                     /gene="ldcC"
                     /locus_tag="RH058_000548"
     CDS             51570..53711
                     /gene="ldcC"
                     /locus_tag="RH058_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV3136187.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53768..54157
                     /locus_tag="RH058_000549"
     CDS             53768..54157
                     /locus_tag="RH058_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3136188.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54222..55520
                     /gene="tilS"
                     /locus_tag="RH058_000550"
     CDS             54222..55520
                     /gene="tilS"
                     /locus_tag="RH058_000550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV3136189.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55569..55829)
                     /gene="rof"
                     /locus_tag="RH058_000551"
     CDS             complement(55569..55829)
                     /gene="rof"
                     /locus_tag="RH058_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV3136190.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55816..56016)
                     /locus_tag="RH058_000552"
     CDS             complement(55816..56016)
                     /locus_tag="RH058_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV3136191.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56182..56727
                     /locus_tag="RH058_000553"
     CDS             56182..56727
                     /locus_tag="RH058_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV3136192.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56724..57146
                     /gene="arfB"
                     /locus_tag="RH058_000554"
     CDS             56724..57146
                     /gene="arfB"
                     /locus_tag="RH058_000554"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV3136193.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57160..57870
                     /gene="nlpE"
                     /locus_tag="RH058_000555"
                     /gene_synonym="cutF"
     CDS             57160..57870
                     /gene="nlpE"
                     /locus_tag="RH058_000555"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV3136194.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13584 a        14374 c        16140 g        13815 t
ORIGIN      
        1 aaatgctctt gtatgtaaga cttcacgcaa cgtctcccca tcaggaaacg ccagctccgg
       61 tgcaatctca aacagcggcc acagcataaa tccacgattc ttcatatcgt agtgcggaac
      121 ggtcaggcgt tcggtattta tcacttcatt accaaacagc atgatatcga gatccagcgt
      181 gcgtggtccc cagcgttcag ctttacgaac acgaccctgt tgcagttcta tgcgctgtgt
      241 atggttgagt aactcttccg gggcgagcga agtttcaagc gccacggcgg cgttgaggta
      301 atcgggttga tcttgcggcc ccagcggtgg ggtgcggtaa aacgaagaaa cggcaagaat
      361 gtggctttca gggatatcgc ctaatgcttt cagggcagca ttgacctgct ccagcggaga
      421 ggccagattg ctgcctatgg caatatacgc cactgtcatg cggtaccctc acgacgcggt
      481 gcgcgtttgc gtggacgacg agtacgacga cgcggtgacg gttcttcatc cagctcgtta
      541 agcatccctt tttgatctgg tggtgcggaa acctggaact caccccacca tttcaccaga
      601 cgctgcagtt cagcattacg ttcaacttca gctcgcaagg ccaacaggtc ataagccgca
      661 cggaacttag gatgctccag cagtttccat gcgcgtttac cctgacgacg ggacatacgc
      721 aactgcaact gccagatatc gcgggttaac gtcgtcagac gtttcgggat tgccagtgaa
      781 cggcaggctt cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg ataggtcagg
      841 ccgctttcct gggcgatctt ctgtgccgtc tccagcagtg ggtaccagaa catggcggca
      901 aacaagaacg ccgggttcac gcgcatatcg ttatggatac gcgtatcggt attcttcagc
      961 acctgttcaa tgatccgctc catcgggctg tcgccatttt ccgtgaagta gcgggtaatg
     1021 gtcgggaaca gcggctggaa cagatgatat tcacacaaca gcttataggt ttcgtaaccg
     1081 tagcccgctt gtagcagttt aagcgattct tcaaacaggc gtgccggtgg gatatcgttc
     1141 agcagggtag cgaggcgagg gatcggttct gcggtttccg ggctgatgcg catacccaat
     1201 ttggcggcaa aacgtaccgc gcgcagcata cgtaccggat cttcacggta gcgcgtttcc
     1261 gggttaccaa tcagacggat aacgccgtcc ttcagatcct tcataccgcc aacgtaatca
     1321 cggacggtaa aatccgctac gctgtaatag aggctgttga tagtgaaatc gcggcgctgg
     1381 gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt
     1441 tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt tgcaacttcg
     1501 ataatctccg ggccaaacat cacatgagcc agacggaaac ggcgacccac caggcggcag
     1561 ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc
     1621 ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc
     1681 gctttgttga gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata
     1741 gcatgctgct cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg
     1801 ctttcctcgc ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac
     1861 ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg
     1921 acgcatttga gaatgttgaa tttacaattg ccgactcggg cacggcggta agccgccagt
     1981 ttttgacggc tgactgaagg atttgctcga cgctgaaatc ctgccagtgt gcttctgcct
     2041 gctgccccag aaattgaagt gccgcgatta gtaccgggcg tggatcgcct ttcggcaacg
     2101 caggcgcatg attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat
     2161 gaatgtaatc tggcactttc cagccaaaaa gctggtacag cgagatttgc cttactgttg
     2221 gttcaatcag atcagcccca cgcactattt ctgtaacgcc ctggaaatga tcatcaacca
     2281 caacagccag gttgtaggcg aacaacccat cacggcgatg aatgataaaa tcttcccgtg
     2341 ccagtttttc gtcggcgtga ataatgccgc gcagctggtc agtaaattgc gtgaccggat
     2401 gctgctggcg gatacgcact gcggcattgt ctggtccatg atgcaacacc cggcaatgac
     2461 cgtcgtaaat accgccaatg ctttgaatac gcgcacgcgt acaggtgcag taataactta
     2521 gtccttgttc atgtaaccag gcgagtgctt cacgataggc gtggtgacgt tgcgattgcc
     2581 agagaacatc gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg
     2641 cggcaccggg aacttcacga ggcgggtcga tatcttctat gcgtaccagc cagcgacctt
     2701 gccgggcgcg agcctgcaaa tagctgccga gcgcggcgat cagagagcca aaatgaagct
     2761 cgccggaagg agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca
     2821 aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc
     2881 cagccatctg tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg
     2941 tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc gcaggattcg cagtagccga
     3001 aatcttcgtc ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc
     3061 gatcgcggtt acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt
     3121 ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga
     3181 gttgattacg ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat
     3241 actcttcgcc cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg
     3301 acgatgtttt acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat
     3361 ccccatgttc gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa
     3421 ttatctaaac gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag
     3481 aatgtaaatt aaccagttac ttactttact acaatgtaac cggcagtgat tttttaagag
     3541 ccatgccttc agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag
     3601 cttctgacaa tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa
     3661 tggctgaatg cagcacggcg aaaaagataa ccgcacgctg gccttcagcc gctacgctca
     3721 tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt
     3781 cgttctccgc taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct
     3841 gcaacataaa gtcaatacgg ctgcgttctg cgccgtattt tacttcgctt ttcagcgagc
     3901 tatagcctga cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag
     3961 cccaaagcgt gttgacgcaa ataaatgcgc cgctctggct ttgagttaat tcccaggtgt
     4021 gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccaggcgttg
     4081 cacaaccggt cattgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg
     4141 tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg
     4201 gagagaattc cattgcgaca atccttgtta ttgcgttagc gcccagcgtt ttagcggcgt
     4261 gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata
     4321 cgaccagtta aaacctggcg gcgggattgt caccgcctct ctggcgtcgc gcaataaggt
     4381 aatatgtgga tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg
     4441 gagcatattc gccagctgga ttaagccgcg tggcggctga cgcatcccta accacaccac
     4501 acgcgaacgc agccattgtc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat
     4561 ccgtccggct aaaagagaaa gcgccttctc tttctcggcg ctcacttcgc ctaaaaatgc
     4621 cagagtcaga tgcaaattat cggcggcgac cggacgtccc gcctcaggtg ggaagtgtgt
     4681 ggcgcgccaa tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag
     4741 acgttgcggt tcagacatgt gaggcactcg gttatgaatt accgcgatgc tacaatgtgg
     4801 cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtctt
     4861 acctgaatta cttaccgctc tcgattgtgc gccgcaggta ttattaagtg cgccgaccgg
     4921 ggccgggaaa tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa
     4981 aattatcctg ctggagccgc gtcgtctggc ggcgcgtaac gtcgcgcaac ggctggcgga
     5041 gctgcttaac gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt
     5101 cgggccgaat acccgcctgg aagtggttac cgaaggcgtg ctgacgcgca tgatccagcg
     5161 tgacccggaa ctgagcggtg ttggactggt gatccttgat gaatttcatg agcgcagctt
     5221 gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct
     5281 taaactgctg attatgtcgg ctaccctgga caacgaccgc ttgcagcaaa tgctgccaga
     5341 agcgcctgtc gtcatctcag aagggcgctc gtttccggtt gaacgccgtt atttaccgct
     5401 gcccgcgcat cagcgttttg acgatgccgt tgcggtagcc accgctgaaa tgctgcgtca
     5461 ggaaagcgga tcattactgt tatttttacc tggcgtcgga gaaattcagc gtgtgcagga
     5521 acaactggct tcgcgcatcg gcagtgatgt attgctctgc ccgctgtatg gcgcgttgtc
     5581 gctgaacgat cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct
     5641 ggcgaccaat attgctgaaa ccagtttaac cattgaaggt attcgtctgg tggtggattg
     5701 tgcccaggag cgtgtggcgc gttttgatcc gcgcacgggg cttacgcgac tgattactca
     5761 acgcgttagc caggcatcca tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg
     5821 tatctgcctg catttaatcg ccaaggaaca agcagaacgc gccgcggcgc aaagtgaacc
     5881 ggaaatctta caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag
     5941 cgatccggcg cagatgagct ggctggatca accgccaacg gtgaatctac tggccgcgaa
     6001 acgcctgtta caaatgctgg gggcgctgga gggggaacga cttagtgcgc aggggcaaaa
     6061 aatggcagcg ctgggtaacg atccgcgttt agcggcaatg ctggttagcg cgaagaacga
     6121 cgacgaagct gctaccgcgg caaaaattgc cgccattctc gaagagccgc cacggatggg
     6181 caatagtgac ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca
     6241 gcaactgtta aaacgcttaa acgtacgtgg cggtgaggca gacagttcgc ttatcgcgcc
     6301 gctacttgcc ggggcgtttg ccgatcgcat tgctcgtcgc cgtgggcaag atggacgcta
     6361 tcaactggca aacggcatgg gagcgatgct cgatgccaac gacgcgctaa gccgccacga
     6421 atggttgatc gcaccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact
     6481 ggcgctgctg gtcgatattg atgagttagt acaacgctgc ccgcagctgg tacagcagtc
     6541 tgacactgtg gagtgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctacaaat
     6601 cggtcagttg acggtgaaag tgcagccgct ggcgaaaccg tcagaagacg agttgcatca
     6661 ggcgatgctt aatggcatcc gtgataaagg tttaagcgtg ctcaactgga cggcggaagc
     6721 ggaacagcta cgcttgcgtt tgttatgcgc cgcaaagtgg ttgccggaat atgactggcc
     6781 agcggttgat gatgaaagtt tattggcagc gctggaaacg tggctgctgc cacatatgac
     6841 tggcgtacat tcactacgcg gcctgaaatc actcgacatt tatcaggcac tacgcggatt
     6901 acttgattgg ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc
     6961 gacgggaagc cggatcgcca ttcgttatca tgaagataac ccgcccgcgc tggcggtgag
     7021 aatgcaagag atgtttggcg aggccaccaa tccgacgatc gcccaggggc gcgtgccgct
     7081 ggtgctggag ttgctttcac ctgcccaaag gccattacaa atcacacgag atttgagcgc
     7141 cttctggaaa ggagcgtacc gtgaggtgca aaaagagatg aaagggcgtt atcccaaaca
     7201 tgtctggccg gacgacccgg caaatactgc accgacgcga cggacgaaaa agtattcgta
     7261 aggttggttt tctccctctc cctgtgggag agggccgggg tgagggcatc agcgcgcacg
     7321 ttcaccctca ccctaaccct ctccctcaag ggagagggga ccgatcgagc acaaattttg
     7381 agagatatct tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt gcgcctgaat
     7441 attgcggaga aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaacc
     7501 gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg atgattacga
     7561 cgattatgat gactatgagg atgaagaacc gatgccgcgc aaaggtaagg gcaaaggcaa
     7621 agggcgtaag cctcgtagca aacgcggctg gctatggcta ctgctaaaac tggctatcgt
     7681 ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat caaaaaattc gtagccgtat
     7741 tgatggcaag gtctggcaac tgcctgcggc agtttatggc cgaatggtca atcttgagcc
     7801 agatatgacc atcagcaaga acgagatggt gaagctgctg gaggcgactc agtatcgtca
     7861 ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag gccaacagca ttgagatgat
     7921 ccgccgtccg tttgatttcc cggacagtaa agaagggcag gtgcgtgcgc gtctgacctt
     7981 tgatggtgat catctggcga cgatcgtcaa tatggagaac aaccgtcagt tcggtttctt
     8041 ccgtcttgat ccgcgcctga tcaccatgat ctcttcgcca aacggtgagc agcgtctgtt
     8101 tgtgccgcgc agtggtttcc ctgatttgct ggtggatact ttgctggcga cagaagaccg
     8161 ccatttctac gagcatgatg ggatcagcct ctactcaatt gggcgtgcgg tgctggcaaa
     8221 cctgaccgca ggtcgcacgg tgcagggtgc gagtacgctg acgcaacagc tggtgaaaaa
     8281 cctgttcctc tccagcgagc gctcttactg gcgtaaagcg aacgaagcct acatggcgct
     8341 gatcatggac gcgcgttaca gcaaagaccg tattcttgag ctgtatatga acgaggtgta
     8401 tctcggtcag agcggcgaca acgaaatccg cggcttcccg ctggcaagct tgtattactt
     8461 tggtcgcccg gtagaagagc taagcctcga ccagcaggcg ctgttagtcg gtatggtgaa
     8521 aggggcgtcc atctacaacc cgtggcgtaa cccaaaactg gcgctggagc gacgtaatct
     8581 ggtgctgcgt ctgctgcaac agcaacagat tattgatcaa gaactctatg acatgttgag
     8641 tgcccgtccg ctgggggttc agccgcgcgg tggggtgatc tctcctcagc cagcctttat
     8701 gcaactggtg cgtcaggagc tgcaggcaaa actgggcgat aaggtaaaag atctctccgg
     8761 cgtgaagatc ttcactacct ttgactcggt ggcccaggac gcggcagaaa aagccgccgt
     8821 ggaaggcatt ccggcactga agaaacagcg taagttgagc gatcttgaaa ctgcgattgt
     8881 ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtc ggaggttctg agccgcagtt
     8941 tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg caaaaccagc
     9001 gacttatctg acggctttaa gccagccgaa aatctatcgc ctgaacacgt ggattgccga
     9061 tgcgccaatc gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga acgatgaccg
     9121 tcgttatagc gaaagcggca gagtgatgct ggtggatgcg ttgacccgtt cgatgaacgt
     9181 gccgacggta aatctgggga tggcgctggg gctgcctgca gttacggata cctggatcaa
     9241 actgggcgta ccgaaagatc agctgcatcc ggttccggcg atgctgctgg gggcgttgaa
     9301 cttaacgcca atcgaagtgg cgcaggcgtt ccagaccatc gccagcggtg gtaaccgtgc
     9361 accgctttct gcactgcgtt cggtaatcgc ggaagatggc aaggtgctgt atcagagctt
     9421 cccgcaggca gaacgcgctg ttccggcgca ggcggcgtat ctgacactat ggaccatgca
     9481 gcaggtggta caacgtggta cgggtcgtca gcttggggcg aaatacccga acctgcatct
     9541 ggcagggaaa acagggacta ccaacaataa cgtagatacc tggtttgcag gcattgacgg
     9601 cagcacggtg accatcacct gggtaggccg tgataacaac cagccgacca aactgtatgg
     9661 agccagcggg gcaatgtcga tttatcagcg ttatctggct aaccagacgc caacgccgct
     9721 gaatcttgtt ccgccagaag atattgcaga tatgggcgtg gactacgacg gcaactttgt
     9781 ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat cgctgtgcca
     9841 gcagagcgag atgcagcagc agccgtcagg caatccgttt gatcagtctt ctcagccgca
     9901 gcaacagccg caacagcaac ctgctcagca agagcagaaa gacagcgacg gtgtagccgg
     9961 ttggatcaag gatatgtttg gtagtaatta acatctaagc gtgaaatacc ggatggcgag
    10021 ttgccatccg gtaaaataac atcccatcta agatattaac cctttctttt catctggttg
    10081 tttattaacc cttcaggaac gctcagattg cgtaccgctt gcgaacccgc cagcgtttcg
    10141 aatattatct tatctttata ataatcattc tcgtttacgt tatcattcac tttacatcag
    10201 agatatacca atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc
    10261 agttgtagta gccacagcgg taagcggcat gtctgtttat gcacaggcag cggttgaacc
    10321 gaaagaagac actatcaccg ttaccgctgc acctgcgccg caagaaagcg catgggggcc
    10381 tgctgcaact attgcggcgc gacagtctgc taccggcact aaaaccgata cgccgattca
    10441 aaaagtgcca cagtctattt ctgttgtgac cgccgaagag atggcgctgc atcagccgaa
    10501 gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc
    10561 caacacctat gaccacctga tcattcgcgg ctttgcggca gaaggccaaa gccagaataa
    10621 ctatctgaat ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgacccgta
    10681 tatgctggaa cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag
    10741 tcctggcggc ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc tgaaagaagt
    10801 tcagtttaaa gccggtactg acagcctgtt ccagactggt tttgacttta gcgattcgtt
    10861 ggatgatgac ggtgtttact cttatcgcct gaccggtctt gcgcgttctg ccaatgccca
    10921 gcagaaaggg tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga
    10981 tgataaaacc aattttacct tcctttctta cttccagaac gagccggaaa ccggttatta
    11041 cggctggttg ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac
    11101 agactttaat gaaggggcga agaacaacac ctattctcgt aatgagaaga tggtcggcta
    11161 cagcttcgat cacgaattta acgacacctt tactgtgcgt cagaacctgc gctttgctga
    11221 aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc
    11281 ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa ggccattatc tggcacgtaa
    11341 atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa
    11401 gtttgccact ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg
    11461 taatgacatc aacgcctggt ttggttacga cgactctgtg ccactgctca atctgtacaa
    11521 tccggtgaat accgatttcg acttcaatgc caaagatccg gcaaactccg gcccttaccg
    11581 cattctgaat aagcagaaac aaacgggcgt ttatgttcag gatcaggcgc agtgggataa
    11641 agtgctggtc accctgggcg gtcgttatga ctgggcagat caagaatctc ttaaccgcgt
    11701 tgctgggacg accgataaac gtgatgacaa acagtttacc tggcgtggtg gtgttaacta
    11761 cctgtttgat aatggtgtaa caccttactt cagctatagc gaatcgtttg aaccttcttc
    11821 gcaagttggg aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg
    11881 cgtgaaatat gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac
    11941 taaaaccaac aacctgatgg cggaccctga gggttccttc ttctcggttg aaggtggcga
    12001 gatccgcgca cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt
    12061 agtcggttct tatacttaca ccgatgcgga atacaccacc gatactacct ataaaggcaa
    12121 tacgcctgca caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga
    12181 cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg
    12241 tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta
    12301 tgatctggcg cgagtcggca tggcgggctc caacgtggcg ctgcatgtta acaacctgtt
    12361 cgatcgtgaa tacgtcgcca gctgctttaa cacttatggc tgcttctggg gcgcagaacg
    12421 tcaggtcgtt gcaaccgcaa ccttccgttt ctaatttctc ttttggggca cggattaccg
    12481 tgcccatttc acaagttggc tgttatgcag gaatacacga atcattccga taccactttt
    12541 gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta
    12601 acctttcctg ccgggaaagt gaccggtctg atcggtcaca acggttctgg taaatccact
    12661 ctgctcaaaa tgcttggccg tcatcagcct ccgacggaag gggagattct tcttgatacc
    12721 cagccgctgg aaagctggag cagcaaagcg ttcgcccgca aagtggctta tttgccgcag
    12781 cagcttcctc cggcagaagg gttgaccgtg cgtgaactgg tggcgattgg tcgttacccg
    12841 tggcatggcg cgctggggcg ctttggggcg gcagatcgcg aaaaagtcga ggaagctatc
    12901 tcgctggttg gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa
    12961 cgtcaacggg cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac
    13021 gaaccgactt cggcgctgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt
    13081 ttaagtcagg aacgtggcct gacggtcatt gccgtgctgc acgatatcaa tatggcggca
    13141 cgctactgtg attatctggt cgccctgcgc ggtggtgaaa tgattgctca gggaacgcct
    13201 gcggaaatta tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattttg
    13261 ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta
    13321 tttcgcgccg tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata
    13381 ccgcccacgc ggcggctatt gaccccaatc gtattgtggc gctggagtgg ttgccggtgg
    13441 aattactgct ggcgctcggc atcgtgcctt acggcgtggc ggataccatc aactatcgcc
    13501 tgtgggtcag cgaaccacca ttgccggact cagtgattga cgtcggtttg cgtacagaac
    13561 ctaaccttga actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg gcaggatatg
    13621 gcccttcacc agaaatgctg gctcgtattg cgccggggcg tggatttaac ttcagcgacg
    13681 gcaaacagcc gctggcgatg gcgcgtaagt cactgacgga aatggcagat ttacttaacc
    13741 tgcaaagcgc agcagaaacg catttagcgc actatgaaga ctttatccgc agcatgaaac
    13801 cccgctttgt gaagcgtggt gaacgcccgt tattgctgac gacgcttatc gatccgcgcc
    13861 atatgctggt tttcggtcca aacagcttgt ttcaggaaat tcttgatgag tacggcatcc
    13921 caaatgcctg gcaaggggaa accaacttct ggggcagtac cgccgtcagc atcgatcgtc
    13981 tggcggcgta taaagatgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg
    14041 acgcgctaat ggcaaccccg ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc
    14101 agcgcgtacc cgcagtctgg ttttatggtg cgacgctctc ggcaatgcac tttgtgcgcg
    14161 ttctggataa cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt
    14221 attgctggcg ctgctggtga ttgtcgctac ggcgctgacc tggatgaact tttcgcaggc
    14281 gctgccgcgt agccagtggg cgcaggccgc ctggtcgccg gatattaacg tcatcgagca
    14341 gatgattttt cactacagct tgttaccacg tctggcgatt tctctactgg tgggcgcggg
    14401 cctggggctg gtgggcgtgc tgtttcagca agtgctgcgt aacccgctgg cggaaccgac
    14461 gacgttgggc gttgctacag gcgcgcaact ggggattacc gtcaccacgc tctgggcgat
    14521 cccaggtgct atggcgagcc agtttgctgc gctggcaggg gcttgcgttg ttggcttaat
    14581 tgtctttggc gtcgcgtggg ggaaacggct gtcgccggta acgctgattc tcgcggggct
    14641 ggtagtgagc ctttattgtg gggcaatcaa tcagttactg gttatcttcc atcatgacca
    14701 gttgcaaagc atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggcgt
    14761 tgagcgttta tggccgcagt tgctgggcgg cgtgatgctg acgttactgc tacttcgtcc
    14821 gttaaccctg atggggcttg atgatggcgt ggcgcgcaat ctcgggctgg ccttgtcgct
    14881 cgcgcgtctg gcggcgctgt cgctggcgat agtcatcagt gcgctgctgg tgaacgcagt
    14941 ggggattatc ggctttatcg ggttgttcgc gccactgctg gcaaaaatgc tgggggcgcg
    15001 gcgtttgttg ccacggttga tgctggcgtc gctgattggt gcgttaattc tgtggctttc
    15061 tgatcaaatc atcctctggc tgactcgcgt gtggatggaa gtttccaccg gttcggtcac
    15121 tgcgttgatc ggtgcgccgc tgctactgtg gctgctgccg cgtttacgca gcattagcgc
    15181 gccggatatg aaggtcaacg atcgtgtcgc gactgaacgc caacatgtgc tggcgtttgc
    15241 cctcgcgggc ggcgtgctgc tgttgatggc tgtggtagtg gcgctgtcgt ttggtcgtga
    15301 tgcgcacggc tggacgtggg cgagcggggc gttgctcgag gatttaatgc cctggcgctg
    15361 gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg gcggtagcgg gctgtattat
    15421 tcagcggctg accggcaacc cgatggcaag cccggaagtg ctggggatta gctccggcgc
    15481 agcgtttggc gtggtgttga tgctgtttct ggtgccgggt aatgcctttg gctggctgtt
    15541 acctgcaggc agtctcgggg cggcggtgac gctgttgatc attatgatcg ccgccggacg
    15601 cggtggattt tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcgttcac
    15661 catgcttttg atgatgttgc aggcaagtgg tgacccgcga atggcgcaag tgctgacctg
    15721 gatttccggt tcgacctaca acgcgaccga tgcgcaggtc tggcgcacgg gaattgtgat
    15781 ggtaattttg ctggcgatta ccccgctgtg ccgccgctgg ctgaccattt taccgctggg
    15841 tggtgatacc gcccgagccg taggaatggc gctgacgccg acgcgaattg cgctgctgct
    15901 gttagcggct tgcctgacgg cgaccgcgac gatgactatt ggaccgttga gttttgttgg
    15961 tttaatggca ccgcatattg cgcggatgat gggctttcga cggacgatgc cacacatcgt
    16021 aatttcggcg ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt
    16081 gctgtttcca ttccagatcc cggcggggct gctgtcaacc tttatcggcg cgccatattt
    16141 tatctatttg ttgagaaagc agagccgtta agagaatgcc tcgcccggtg ggcgaggcaa
    16201 aatcatcaca acttcgcaaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc
    16261 atgctgtgcg ccacggacat aaagcccgct tcaaacgctg acggtgccag gtaaacacct
    16321 tcgtccagca tcatatggaa gaaacgctta aagcgttcca cgtcacaggc catcacatcc
    16381 tgatagcacg tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg
    16441 ttaacgacca gcggaattcc ggcttctttt gccgcttcca gcagaccttc tgccagacgt
    16501 gttgtcagct catccagcgt ttcgtgaacg cccggctgcg cgacttcatt cagacaggcg
    16561 aaacccgctg ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggaccc
    16621 gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca
    16681 ccgccgatga ttttgccgag gcaggttaaa tctggcacta cgccgtaata atcctgtgca
    16741 ccagccagtg ccacgcggaa gccagtcatt acttcatcga tgatcagcaa tgcgccaaat
    16801 tcgtcgcaca gcgcgcgcag acctggcagg aactctggca gcggcggaac acagttcata
    16861 ttgcctgcca ccggctcaac gataatacag gcaatctctt gcgggtattg ctcaaacgcg
    16921 gcgcgtacag aagccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc
    16981 ggaacacccg gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc
    17041 aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa tgattttgtc gcgaccggta
    17101 aaaccacggg ccaggcggat ggcgctcatg gtcgcttcag tgccggagtt caccatgcgc
    17161 accatatcca tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg
    17221 gttggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggattgcc
    17281 ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg
    17341 ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc
    17401 acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg
    17461 ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc
    17521 tgctattcta tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact
    17581 ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc
    17641 aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag
    17701 tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg
    17761 acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt
    17821 tatggcggca gtcgtcggca cgcttgtcgg gctggcagcg gttgcttttg acaaaggtgt
    17881 cgcctggttg cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct
    17941 actgttaacc gtcgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt
    18001 gcgcaaatac gcgcccgaag cagggggatc ggggatcccg gaaattgaag gggcgctgga
    18061 agatcaacgt cctgttcgct ggtggcgtgt attgccggtg aaattctttg gcgggctggg
    18121 gacactcggc ggaggcatgg tgctagggcg cgaaggacca accgtgcaga tcggcggcaa
    18181 tatcggtcgc atggtgcttg atattttccg cctgaaaggt gatgaagctc gccatacgct
    18241 gctggcaacc ggtgctgctg cggggctggc tgcggccttt aacgcgccgc tggcgggtat
    18301 tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc
    18361 ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc
    18421 gttgattgac gtcggtaaac tttctgatgc gccgcttaat acgctgtggc tttatctgat
    18481 cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca
    18541 ggatttgctg caccgtgtgc acggcggcaa tattaccaaa tgggtactga tgggtggtgc
    18601 gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt
    18661 taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt
    18721 cgtcgctcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt
    18781 tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga
    18841 gctgtttccg caatatcacc tggaggcggg gacgtttgct attgccggaa tgggggcatt
    18901 actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga
    18961 taactaccag ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca
    19021 atttaccggc gggaaaccgc tatactcggc gattcttgcg cgcacgctgg caaaacagga
    19081 agctgagcaa ctggcgcgaa gcaaggctgc atcagccagc gagaatactt gaacgaaata
    19141 ccagggtatt agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg
    19201 tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc
    19261 caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta
    19321 tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa
    19381 cgaaggcgat atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct
    19441 gcaatatctg gtcggcggtt ccgttgacta caccgaagga ctggaaggtt ctcgtttcat
    19501 cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc
    19561 tgttgttcat gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca
    19621 tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc
    19681 gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc
    19741 gtagcgtgga caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc
    19801 atggggatct cgcgggccag tacatcacga atgatcccgc cgccaacgcc agtaatgacg
    19861 cccatacaaa ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg
    19921 ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt
    19981 cttggctggc gcaccagcac gatggtcagc atgctggtga ccattgcaac gaccagatcg
    20041 gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgttccg
    20101 ccgcctactg cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt
    20161 ccggctaaca aaacgccgga gatggcaaat accgctgtgc cgactatatc cagccaatag
    20221 acgagcattg ttcaatcccc actgagcacc tgaaaaggtc aggcactaat ctacctgtga
    20281 aagcgcatta cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca
    20341 gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt
    20401 aggaatttgg tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg ctaacacctg
    20461 ttcgcggcta acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca
    20521 aacttcgagt acctggttct gaatcgactc ttttccactg gtaaatggcg gattaatgcc
    20581 gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta
    20641 ctgatccagc agggattgcg cggcttgttc ggccttgtct ggttgcggac tccagggggc
    20701 cagttgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt
    20761 tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat
    20821 caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac
    20881 ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt
    20941 gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc
    21001 ggcgttgagc cacagtggcg caagaaaaga cagggcgacc agcgccctga acagtgactt
    21061 agccatgtgc aagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa
    21121 cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc
    21181 gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca
    21241 tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt
    21301 tgatgaaagc gtcgccgcta acgatcaggc cacgtacagc gttaagattc agttcggcaa
    21361 tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg
    21421 gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgtgcttcgt
    21481 ccgagacaac gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt
    21541 taataatcac atctggcttg cagtgttcca acagcaaagt ggcacccagc gccgcagcga
    21601 ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga
    21661 tttcgcaacc gcccagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa
    21721 cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga
    21781 tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac
    21841 atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag
    21901 ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc
    21961 aactctccgg caattcgtcg tctgcaacaa aagacgcagg tttttccact ggagcgcaat
    22021 gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc
    22081 gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat
    22141 gaactgaccg gaccttttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc
    22201 aatccgccgt ttggtcattt tggcgaagca gcgataaatg actggtttcg ccaacgtttg
    22261 cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggcacta
    22321 cgtttacggg acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta
    22381 tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat
    22441 ctcacctggg cacaggttgg cggtatttta aaatataccc gtccggcgtg gtggcgtggc
    22501 gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa
    22561 gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta
    22621 acgtggatta tggaagctgc cgacgatatc tcctattgtg tggcagacct tgaagatgca
    22681 gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga agcgtggggc
    22741 cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc
    22801 tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc
    22861 ctaaataaac tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc
    22921 gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag
    22981 ctatataaaa atgtcgctgt aaaacatgtg tttagccatc cagatgtcga gcagcttgaa
    23041 ttgcagggct atcgggtcat tagcggatta ttagagattt atcgtccttt attaagcctg
    23101 tcgttatcag actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgaa
    23161 tcgcgcttat tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt
    23221 aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg
    23281 ctgcaggatt atatcagcgg tatgaccgac ctctatgcgt gggatgaata ccgacgtctg
    23341 atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc
    23401 agaaacttta gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc
    23461 gttatctgtt aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg
    23521 gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca
    23581 gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca
    23641 gtggtcagca ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat
    23701 ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc
    23761 tctccgttct gccagggtgg ccagggcggt aatggtggcg gccagcaaca gaaattcatg
    23821 gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac
    23881 gttgttgata acgcgacggt cattaaagtt caactgagcg atggccgtaa gttcgacgcg
    23941 aagatggttg gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaacccgaaa
    24001 aacctgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta
    24061 gcgattggta acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg
    24121 gggcgtagcg gcctgaatgc cgaaaactac gaaaacttca tccagaccga tgcagcgatc
    24181 aaccgtggta actccggtgg tgcgctggtt aacctgaacg gtgaactgat cggtatcaac
    24241 accgcgatcc tcgcaccgga cggcggcaac atcggtatcg gttttgctat cccgagcaac
    24301 atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg
    24361 ggtattatgg ggactgagct gaactccgaa ctggccaaag cgatgaaagt tgacgcccag
    24421 cgcggtgctt tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa
    24481 gcgggtgatg tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt
    24541 gctcaggtgg gtactatgcc ggtgggcagt aaactgaccc tgggcttact gcgcgacggt
    24601 aagcaggtta acgtgaacct ggaactgcag cagagcagcc agaatcaggt tgattccagc
    24661 tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg
    24721 gtagtgaaca acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat
    24781 gtgattattg gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc
    24841 gacagcaaac cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg
    24901 ttaatgcagt aatctccctc aaccccttcc tgaaaacggg aaggggttct ccttacaatc
    24961 tgtgaacttc accacaactc catacatctt catcatcctt taggcatttg cacaatgccg
    25021 tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca
    25081 tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa
    25141 tatcaacgta atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg
    25201 tgaattgcac gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga
    25261 cgcggtggca cgtcatctgc acggtgtgcg gcaagggatt aatctaccgt tacggctgga
    25321 aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg
    25381 cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacctgtt
    25441 ggcgcaggat agccgtttgc gggaagaact ggtgatgaac ctgattcagg ctgaggagaa
    25501 tactcccgca cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt
    25561 agtggctatt gttgaggtcg acagcggtca gcttggcgtg gacagcgcaa tggcggagtt
    25621 acaacaactg caaaatgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc
    25681 gctgaccgaa atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgcaga
    25741 agatcatcgt aagcgagttg aacaactgat tacccgcatg aaagagtatg gccagctgcg
    25801 ttttcgcgtt tcgctgggca actattttac cggtcctggc agtattgccc gatcctatcg
    25861 tacggcgaaa acgacgatgg tggtgggtaa acagcggatg ccagaaagtc gctgctattt
    25921 ttatcaggat ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa
    25981 tgaactggcg cgaccgctgg cgcggctgaa agcgatggac aataatggct tgctgcgacg
    26041 aacgctggcg gcgtggtttc gccacaatgt gcaaccgctg gctacgtcaa aggcgctgtt
    26101 tattcatcgt aatactctgg agtatcggct taatcgtata tcggaactga ccgggcttga
    26161 tttgggtaat tttgatgaca ggttgctgct gtatgtggcg ttgcagctgg atgaagagcg
    26221 gtaggttatg cgttaaggtc gaggcggcgc tggctcattc ccctcagccc gaagggtaga
    26281 ggggaatgtc cgagttaacc gtcagttcgg cattatttac gcgttagttt ttccagatcg
    26341 gcttcaatct cgctgatctt attggttacg actgactcca ggtgacgtaa gtcgtcgagg
    26401 atcttacgct taagatcaac ttcgctgcgg tcacgctggc agatttgatc aagctcatca
    26461 atgatatacc gtagattcgg gctgatttcc tggacttctt tataaccctg acccacacca
    26521 tcagcgacga ccgttttacg ctgacgcgga tatttaaact taacgctctt ggcgaaaaac
    26581 tcgcctttgt ctttctggaa atagattttc agaatatcgt tgttggcttc ctgccggagg
    26641 ctgtaacgat caatttcttc aggattggta atacccagac ttttcagatt gtcgtacata
    26701 gcgttacctc aaaatgagtc agtaaattgt gcttatttta gcatttggcc tggcccgccc
    26761 ggctcgactg tgatcgcaga aagccttttt cttccagact tctatcatgg cgtaaaaatc
    26821 aaaaaattac ctgctttatt gtggcgatat tccatgggat ggattaacac atttatcttc
    26881 gttttgcgaa ttacatgaca gtccgaaaca agatgaaaaa gtatttaaaa aaggggggaa
    26941 gaagaatgag taggacgaat tattctgagc cagaaagcat acgggcagca tcatgctgcc
    27001 cgtatatgta ctcttaatcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag
    27061 ttttcgcgtc aactttctta acgataaccg cacagtagag gctgtatttg ccatctttcg
    27121 acggcagatt acctgaaaca accacagacc ccgccggaac gcgaccgtag tggatttcgc
    27181 cggtttcacg gtcgtaaata cgggtgctct gaccaatgta tacgcccata gagatgacgg
    27241 aaccttcttc gacaatcacc ccttcaacca cttcggagcg tgcgccaatg aagcagttat
    27301 cttcaataat ggtcgggtta gcctgcagcg gctccagcac gccgccgatg cccacgccac
    27361 cggaaaggtg gacgttttta ccaatctgcg cacaagaacc gacggtcgcc caggtatcaa
    27421 ccatcgtgcc ttcatcaaca tatgcgccga tgttgacgta agacggcatc agcacggtgt
    27481 tacgggcaat aaatgcaccc tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt
    27541 tctgaaaacg cgcttcgtcg tagtcggcga atttcatcgg cactttatcg aagtagcggc
    27601 tttctgcccc ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt
    27661 tcaaccactg atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcgccgg
    27721 aatccagcag ggcgatcacc tgatttaccg cttcgcgggt tacggtgtct gcattggctg
    27781 gcgtgatctc ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg
    27841 ttaaactctt ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg
    27901 tcaaccgctg atgcacttcc tgctgcaact cgttattaag cgcacgccgg tcagcggtgg
    27961 caattatgaa taaatcttct actcgctcgc caatggttgt aattcgggcg ccatgaagcg
    28021 aaattcccag atcggcaaaa attttcccga ctctcgccag cagtcctggt tggtcgagcg
    28081 cgataagctc aaggaacgat ttgcggtcgg tatgggttgg caaaaacgtc acttcggttt
    28141 caacagtaaa gtgacgtagt ttagccggtt gacgacgcgg ctgtggtggc tgccagctac
    28201 tttgtgtcag tacctgctcc agaccaaacc gaataacctc atgacgatct gcggatagcg
    28261 ggctaccatc gggttccagc acgatgaagg tatccatcgc cataccgtcg cgagtggtga
    28321 aaatttgtgc gtcgtgaaca cttaaattgc ggcggtctaa ttcggcacag acggcggcaa
    28381 acagataggg gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg
    28441 ggctaagcaa taccagcggt ttgcttaaat catgctgtaa taaatggcgg gcatgccagg
    28501 ccagttgatt tgggctatgg cggacaaaat agttagcacg acagcgtgac caaatttggt
    28561 gcagcgcctc ttcgtcgatg ttatccatgc gcagtagtgc cagtgcctgg agttggtgat
    28621 ggcgaacccg ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgtttttcgg
    28681 tggcaaaata gagttcgcgc aacagacttt gcttccagct attccacagc gtttcgttgg
    28741 tggcgcaaat gtcagccact gtcaggcata ccagatagcg cagacgattt tccgtttgaa
    28801 cttcttcggc aaactgcttg atgacttcag ggtcctgaat atcgcggcgt tgtgctgtca
    28861 ccgacatcag caagtgctgg cgaaccagcc aggcaaccag ctgtgtttcg cgtgagttca
    28921 gcccgtggag ttctgcaaaa tgcactacat cctgagcacc gagaatggag tggtcgccgc
    28981 cgcgtccttt ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggca
    29041 ggcgcggcca gacgtccaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac
    29101 tctccagttt cagcatcacg cggatagtat gttcatccac cgtgtaggcg tggaacagat
    29161 caaactgcat ctgcccgacg atatgcgacc attgcggcat atacgcgccg agcacgctat
    29221 ggcgatgcat tggcaatagc ccgcgccgca ccgctccggg gtgacgcaga atgctcaaaa
    29281 acagttttcg tgcttccgga atattacaca gcggttgttg cagatggcga cgggcatggc
    29341 gtaactggcg cagcgtggtg gagtaaatgc cggtgatcgc actgttgcgc accatggtgt
    29401 agaacatacg caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga
    29461 ttagcgtacc gcgtagctga aactcatcgt cgattggacg tggtttttcg tcggcgggaa
    29521 gggcgaggat ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc
    29581 gtgtaacgcg gaagtaatcc ttcatcatcc gctcgaccgg ttcgttgcct tcaccactgt
    29641 aattcagacg ctgagcgacg ctaagttggc gatcgaataa caggcgatta tcgtaacggc
    29701 tgacgaccag atgcagggca aagcgaatac gccacagtat atgcagacat tcgtttaatt
    29761 ccgcccgctc cgctgaggtt aagaagccaa acccaaccat ttcatccagc gatgttgcgc
    29821 caaaatgacg gcgggccacc cattgcagag tgtggatatc gcgcaagccg ccagggctac
    29881 ttttgatgtc tggttcaaga ttgtagctgg tgccgtggta acgctgatgg cgctggttct
    29941 gttcttcaac tttcgccgcg tagaacttgt cggaaggcca gaatccttcg ctaaaaatat
    30001 gtttttgcag ttcgaggaag agcgcaacat cgccaattaa taagcgggat tcgattaaat
    30061 tggtggcgac ggttaaatcc gataaccctt ccagcatgca ctcttcaagc gtgcgcacac
    30121 tatgaccgac ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg
    30181 cctgatcgtc cgggagcttt ttacggctta aaatcagtaa atcgatgtca gaaagtggat
    30241 gcagctcgcc acgaccgtag ccaccgacgg cgaccaatgc caggtcggca atctggctga
    30301 atcccgcttc aatccataat cgttgcagga gctggtcgat aaactcggtg cgcgcctcaa
    30361 tcaactgttc tgcagagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat
    30421 cgatatgggc ttttatccca ccgacggtta attcatcacg gggccagacg catggatttt
    30481 gcggttgacc gggcagggtg gggagagcgg tgtttgcgta ctgttctgga agggtattca
    30541 ttgtgcgcca cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca
    30601 tcttattcgt cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt
    30661 tcgcagccgt tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct
    30721 ttggttttta ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc
    30781 attggctcga tggtgaacgt catcccaggt ttcagtacga cgttggtttc acgggagtca
    30841 tagtgcagca cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca
    30901 cgaacgacgg agaagccttc tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc
    30961 agattaatgc ctggtttgac catgcgcagc gccaggtaca ggctttcttg cgtgatgcgg
    31021 cacagacgtt cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg
    31081 tggaaaccat ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc
    31141 ttagcatcgt ccgggatacc gtggcacacc acttcattaa tagagatgca aacggatttc
    31201 ggatagccgt gatagccgag gcaggcagaa accgcgtgtt gttcattaac aatgtaatca
    31261 ttacagatgc gatccagctc gccggtgctg acgcccggtt taacatacgg ttcgatcatc
    31321 tccagcactt cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc
    31381 ttgattgaga tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt
    31441 aagtgccgtc aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac
    31501 gtgccactga aggttttcta taatagaaaa ttcgacatct gatgctgtac acagcgccaa
    31561 caattattgg tgtccatgac gtatttgtgg tataaagcgc gccggacttc cgatccattt
    31621 cgtatacaca gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg
    31681 gcacatattc cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc
    31741 aacttttata tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct
    31801 ggtgttcact tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc
    31861 ggtgcgcgta acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa
    31921 gctctggctg aactgaacaa gattgcttct cgcaaaggta aaatcctttt cgttggtact
    31981 aaacgcgctg caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg
    32041 aaccatcgct ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa
    32101 cgtctgaaag acctggaaac tcagtctcag gacggtactt tcgaaaagct gaccaagaaa
    32161 gaagcgctga tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa
    32221 gacatgggcg gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct
    32281 atcaaagaag caaacaacct gggtattccg gtatttgcta tcgttgatac caactctgat
    32341 ccggacggtg ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg
    32401 tacctgggcg ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag
    32461 gcggaagaaa gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc
    32521 gagttgcaga aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg
    32581 gatgagcgag cgaagataac gcatctgcgg cgcgaaatat gaagggggag agcccttata
    32641 gaccaggtag tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata
    32701 tctgtgcggt ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta
    32761 ccgcatccct ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa
    32821 aagcactgac tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg
    32881 gtgctattaa agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca
    32941 aaatcgacgg caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa
    33001 aagacgctgg tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa
    33061 tcactgacgt tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga
    33121 aaattggtga aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt
    33181 cttatcagca cggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc
    33241 tggttaaaca catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccagaag
    33301 acgtatccgc tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt
    33361 ctggtaagcc gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg
    33421 gcgaagtttc tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc
    33481 tgctgaaaga gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca
    33541 tcgagaaagt tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtcttaat
    33601 tatcaaaaag gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta
    33661 acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa
    33721 tcgttgacag tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat
    33781 tctgcttaag ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag
    33841 catactggat cgtatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg
    33901 tgtggtgatt ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa
    33961 ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta atgaacggcc tggcaatgcg
    34021 tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg
    34081 cgtgtgcgac agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt
    34141 gatcctctcc gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg
    34201 tggtatcgaa attgaagccg atgtggtgct gaaagcaact aaagttgacg gcgtgtttac
    34261 cgctgatccg gcgaaagatc caaccgcaac catgtacgag caactgactt acagcgaagt
    34321 gctggaaaaa gagctgaaag tcatggacct ggcggccttc acgctggctc gtgaccataa
    34381 attaccgatt cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg tggtaatggg
    34441 tgaaaaagaa gggactttaa tcacggaata attcccgtga tggataaata agggtaagat
    34501 tccacgtaag tatcgcgggg gcgttagtct ggttataagg cgttattgtt gcaggcagtt
    34561 tggtcacggc cagcgcgcag caaccggagc gtacaaaagt acgtgaggat ggcgagcact
    34621 gcccggggcc aaaatggcaa ataaaatagc ctaataatcc agacgattac ccgtaatatg
    34681 tttaatcagg gctatactta gcacacttcc actgtgtgtg actgtctggt ctgactgaga
    34741 caagttttca aggattcgta acgtgattag cgatatcaga aaagatgctg aagtacgcat
    34801 ggacaaatgc gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc
    34861 tcccagcctg ctggatggca ttgtcgtgga atattacggc acgccgacgc cgctgcgtca
    34921 gctggcaagc gtaacggtag aagattcccg tacactgaaa atcaacgtgt ttgatcgttc
    34981 aatgtctccg gccgttgaaa aagcgattat ggcgtccgat cttggcctga acccgaactc
    35041 tgcgggtagc gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct
    35101 gaccaaaatc gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta acgtgcgtcg
    35161 tgacgcgaac gacaaagtga aagcactgtt gaaagataaa gagatcagcg aagacgacga
    35221 tcgccgttct caggacgatg tacagaaact gactgatgct gcaatcaaga aaattgaagc
    35281 ggcgctggca gacaaagaag cagaactgat gcagttctga tttcttgaac gacaaaaacg
    35341 ccgctcagta gatccttgcg gatcggctgg cggcgttttg ctttttattc tgtctcaact
    35401 ctggatgttt catgaagcaa ctcaccattc tgggctcgac cggctcgatt ggttgcagca
    35461 cgctggacgt ggtgcgccat aatcccgaac acttccgcgt agttgcgctg gtggcaggca
    35521 aaaatgtcac tcgcatggta gaacagtgcc tggaattctc tccccgctat gccgtaatgg
    35581 acgatgaagc gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggt agccgcaccg
    35641 aagtcttaag tgggcaacaa gccgcttgcg atatggcagc gcttgaggat gttgatcagg
    35701 tgatggcagc cattgttggc gctgctgggc tgttacctac gcttgctgcg atccgcgcgg
    35761 gtaaaaccat tttgctggcc aataaagaat cactggttac ctgcggacgt ctgtttatgg
    35821 acgccgtaaa gcagagcaaa gcgcaattgt taccggtcga tagcgaacat aacgccattt
    35881 ttcagagttt accgcaacct atccagcata atctgggata cgctgacctt gagcaaaatg
    35941 gcgtggtgtc cattttactt accgggtctg gtggcccttt ccgtgagacg ccattgcgcg
    36001 atttggcaac aatgacgccg gatcaagcct gccgtcatcc gaactggtcg atggggcgta
    36061 aaatttctgt cgattcggct accatgatga acaaaggtct ggaatacatt gaagcgcgtt
    36121 ggctgtttaa cgccagcgcc agccagatgg aagtgctgat tcacccgcag tcagtgattc
    36181 actcaatggt gcgctatcag gacggcagtg ttctggcgca gctgggggaa ccggatatgc
    36241 gtacgccaat tgcccacacc atggcatggc cgaatcgcgt gaactctggc gtgaagccgc
    36301 tcgatttttg caaactaagt gcgttgacat ttgccgcacc ggattatgat cgttatccat
    36361 gcctgaaact ggcgatggag gcgttcgaac aaggtcaggc agcgacgaca gcgttgaatg
    36421 ccgcaaacga aattaccgtt gctgcttttc ttgcgcaaca aatccgcttt acggatatcg
    36481 ctgcgttgaa tttatcggta ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggacg
    36541 atgtgttatc tgttgatgcg aacgcgcgtg aagtcgccag aaaagaggtg atgcgtctcg
    36601 caagctgagg ataatccggc tacagagagt cgcgctattt gttagcgtag ggcttcagtg
    36661 atatagtctg cgccatctga tcgtaagtag ttggctttat aaggtcaggt atgccgtggt
    36721 tttacacggc ttttttttgt ataggcttca gtattcctga gtaccgtaaa ccctgtcagg
    36781 gaataaaaaa cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat tgccagcgca
    36841 tggctgccgt catgttgcga tcattatgga cggcaatggt cgctgggcaa aaaagcaagg
    36901 gaagattcgt gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt
    36961 tgcggccaac aacggtattg aggcgttaac gctgtatgcc tttagtagtg aaaactggaa
    37021 ccgaccagcg caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc tcgatagcga
    37081 agtaaaaagt ctgcaccgac ataacgtgcg tctgcgtatt attggcgata ccagtcgctt
    37141 taactcgcgt ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg ggaataccgg
    37201 tctgacgctg aatattgcgg cgaactacgg tggacgttgg gatatagtcc agggagtcag
    37261 gcaactggct gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag atgaagagat
    37321 gctaaaccag catgtctgta tgcatgaact ggcccctgta gatttagtaa ttaggactgg
    37381 gggggagcat cgcattagta actttttgct ttggcaaatt gcctatgccg aactttactt
    37441 tacagatgtt ctctggcccg atttcgatga acaagacttt gaaggggcgt taaatgcctt
    37501 tgctaatcga gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag cctgatgggg
    37561 gtcgcttttg ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc
    37621 ggcgttgttt ctgttgccgc cggtggggtt cgccatcgta acgctggtgg tctgcatgct
    37681 ggcagcgtgg gaatggggac agcttagcgg ttttaccact cgttcgcagc gagtatggtt
    37741 ggcggtgtta tgcgggttat tgttggcgct gatgcttttt ctgttgccgg aatatcaccg
    37801 aaatattcat caaccgctgg ttgaaatctc actttgggct tcgctgggtt ggtggattgt
    37861 cgcgctattg ctggtgctgt tttacccagg ttccgcagca atctggcgta actctaaaac
    37921 attgcgcctt atttttggcg tgctaaccat tgttcccttc ttctggggca tgctggcgtt
    37981 acgggcctgg cactatgacg agaatcatta cagtggcgca atatggctgc tctatgtcat
    38041 gatcctggta tggggcgctg actccggcgc atatatgttt ggcaaattgt ttggtaaaca
    38101 taagctggca ccgaaggttt ctccgggtaa aacctggcaa ggctttatag gtggactagc
    38161 gaccgcagcg gtaatctcat ggggttatgg catgtgggcg aatctcgacg tcgctcccgt
    38221 caccttactc atttgctcta ttgtcgcagc gttagcctca gtgctcggcg atctgaccga
    38281 gagtatgttt aagcgtgaag caggaattaa ggacagcggt catttaattc caggacacgg
    38341 tggtatttta gatcgtattg atagcctgac ggctgcggta ccggtctttg cttgcttgtt
    38401 gttactggta ttcaggacgc tttaacggaa ggtaatatgc tgagttttct ctgggatttg
    38461 gcttcgttca tcgttgcact gggtgtactt atcaccgtgc atgaatttgg tcatttctgg
    38521 gttgcccggc gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc
    38581 tggcggcgaa ctgataagct cggcaccgaa tatgttatcg ccctgatccc gctgggcggt
    38641 tatgtcaaaa tgctggatga gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc
    38701 ttcaataata aatctgtcgg ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac
    38761 ttcatttttg ctatctttgc ctactggctg gtttttatta ttggtgtgcc tggcgtacgt
    38821 ccggtggttg gtgaaatagc agccaattcg atagctgcgg aagcacaaat tgcaccaggt
    38881 acggaactaa aagccgtaga tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag
    38941 ttggtcgata aaattggcga tgaaagcacc accattacag tagcgccatt tggcagcgac
    39001 caacggcggg atgtaaagct cgatttacgt cactgggcgt ttgagcctga taaagaagat
    39061 ccggtatctt cgctggggat tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat
    39121 gtgcaaccaa actcggcggc aagcaaggca ggtttgcaag caggcgacag gatcgttaaa
    39181 gtcgatggtc agcccttaac gcagtgggtg acctttgtga tgcttgtccg ggataacccg
    39241 ggtaaatcct tagcgttaga aatcgaaagg cagggaagtc ccttgtcttt gacattaatc
    39301 ccggagagta aaccgggtaa tggtaaagcg attggttttg tcggtattga gccgaaagtc
    39361 attcctttgc cagatgagta taaagttgta cgccagtatg ggccgttcaa cgccatcgtc
    39421 gaagccacgg acaaaacgtg gcagctgatg aagctgacgg tcagtatgct gggaaaattg
    39481 atcaccggtg atgtgaaact gaacaacctc agtgggccga tctctatcgc caagggggct
    39541 gggatgacag cggaactcgg ggtagtttat tacctgccgt ttcttgcgct tattagcgtg
    39601 aacttaggga taattaacct gtttccgttg cccgtacttg acggggggca tctgctgttc
    39661 cttgcgatcg aaaagatcaa gggcggaccg gtatccgagc gggttcaaga cttttgttat
    39721 cgcattggct cgattctgct ggtgctgtta atggggcttg cacttttcaa tgatttctct
    39781 cggttatgag agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca
    39841 tagcgtcgct gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag
    39901 atattcattt cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg
    39961 tgcgcacagg cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg
    40021 ctaccggcaa ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg
    40081 taaaagaacg tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg
    40141 acatgctgaa gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca
    40201 ccaccattgc cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata
    40261 gcgccagcgt aaaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg
    40321 tgttccagga aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt
    40381 tcaccaccga cgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaacg
    40441 tggtaggcga tcgtaaatac cagaaacaga aactggcggg cgaccttgaa accctgcgca
    40501 gctactatct ggatcgcggt tatgcccgtt tcaacatcga ctctacccag gtcagtctga
    40561 cgccagataa aaaaggtatt tacgtcacgg tgaacattac cgaaggcgat cagtacaagc
    40621 tttctggcgt tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt gagcagctga
    40681 ctaagatcga gccgggcgag ctgtataacg gcaccaaagt gaccaagatg gaagatgaca
    40741 tcaaaaagct tctcggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa
    40801 ttaacgatgc cgacaaaacc gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct
    40861 acgtgcgtaa gatccgtttt gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg
    40921 aaatgcgtca gatggaaggt gcatggctgg gaagcgatct ggtcgatcag ggtaaggagc
    40981 gtctgaatcg tctgggcttc tttgaaactg tcgataccga tacccaacgt gttccgggta
    41041 gcccggacca ggtagatgtc gtctacaagg taaaagagcg caacaccggt agcttcaact
    41101 tcgggattgg ttacggtact gaaagtggcg tgagcttcca ggctggtgtg cagcaggata
    41161 actggttagg tacaggttat gctgttggta tcaacgggac caaaaacgat taccagacct
    41221 atgctgaact gtcggtaacc aacccgtact tcactgtaga tggtgtaagc ctcggtggtc
    41281 gtctcttcta taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga
    41341 gttatggtac agacgtgacg ttgggcttcc cgatcaacga atataactcg ctgcgtgcag
    41401 gtctgggtta tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt
    41461 atctgtactc tatgggtgaa catccgagca cctctgatca ggataatagc ttcaaaacgg
    41521 acgacttcac gttcaactat ggttggacct ataacaagct tgaccgtggt tacttcccga
    41581 cagatggttc acgtgtcaac ctgaccggta aagtgaccat tcctggatcg gataacgaat
    41641 actacaaagt gacgttagac acggcgactt atgtgccgat cgatgacgat cacaaatggg
    41701 ttgttctggg gcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt
    41761 tctacgagaa cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacaa
    41821 ttggtccgaa agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt
    41881 acgaatgtgc gactcaggac ggcgcgaaag acctgtgtaa atcggatgat gctgtaggcg
    41941 gtaacgccat ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata
    42001 agtatgctaa ctcggttcgt acttccttct tctgggatat gggtaccgtt tgggatacaa
    42061 actgggattc cagccaatat tctggatatc cggactatag tgatccaagc aatatccgta
    42121 tgtctgcggg tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg
    42181 cccagccgtt caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta
    42241 aaacctggta agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg
    42301 atcaatataa gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt
    42361 aaggagttta ttgtgaaaaa gtggttatta gcggcaggtc tcggtttagc actggcaact
    42421 tctgctcagg cggctgacaa aattgcaatc gtcaacatgg gcagcctgtt ccagcaggta
    42481 gcgcagaaaa ccggtgtttc taacacgctg gaaaatgagt tcaaaggccg tgccagcgaa
    42541 ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg
    42601 ggcagcgatc gcactaagct ggaaaaagac gtgatggctc agcgccagac ttttgctcag
    42661 aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg
    42721 gttactcgta tccagactgc tgtgaaatcc gttgccaaca gccaggatat cgatctggtt
    42781 gttgatgcaa acgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta
    42841 ctgaaacagg ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg
    42901 gatgcagaac tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct
    42961 gcacaaacag gtcacattac gttcatggtt aacccaaaat accgtgagca tttaggcttg
    43021 tgccaggcgt ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg
    43081 ctggtagtga agaatcccta cctgacttac gcgcgcatgg cgcaaatttt agataccacg
    43141 ccgcagcccg cgcagaacat tgcacccagt gcggtgatcg acgcgacggc gaagctgggt
    43201 aacaacgtat cgattggcgc taacgcggtg attgagtccg gcgttgaact gggcgataac
    43261 gtgattatcg gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt
    43321 ctctgggcga acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag
    43381 tccggaacag tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg
    43441 aagatcccac agattggtcg cgtaattatt ggcgatcgcg tggagatcgg tgcctgcaca
    43501 accatcgatc gcggcgcgct ggatgacact attattggca atggcgtgat cattgataac
    43561 cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata cggcggttgc cggtggcgtc
    43621 attatggcgg gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc
    43681 aacgggcata tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt
    43741 cccatcactg aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg
    43801 cgcaaaaccg ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt
    43861 gagcgcaagg ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc
    43921 tgtctcattc ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca
    43981 ggaagagtat cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc
    44041 tgccgcaccg tttccctttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt
    44101 ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg
    44161 gaaaaccgat tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc
    44221 tggcgtttaa aagcgtagga aaactggaac cgggtgagct gtactatttc gctggtattg
    44281 acgaagcgcg cttcaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt
    44341 tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gacggtaaag
    44401 tagtttgcga agcaacgatg atgtgtgctc gcagccggga ggcctgatac gtgattgata
    44461 aatccgcctt tgtgcatcca accgccattg tggaagaggg cgcgtcgatt ggcgcgaacg
    44521 cacacattgg tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac
    44581 tgaaatctca cgttgtcgtg aatggccata ctaaaattgg ccgcgataat gagatttatc
    44641 agttcgcctc catcggtgaa gttaaccagg atctgaaata tgctggcgaa ccgacccgtg
    44701 tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc
    44761 agggcggtgg attgacgaag gtgggcagcg acaacttact gatgatcaac gcgcacattg
    44821 cgcacgattg tacggtaggt aaccgctgta ttctcgccaa caacgcaacg ctggcgggtc
    44881 acgtatcggt agacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca
    44941 tcattggtgc gcatgtgatg gttggcggtt gctccggtgt ggcgcaggac gtccctcctt
    45001 atgtcattgc gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc
    45061 gccgcggatt cagccgcgag gcgattaccg ctatccgcaa tgcgtataag ctgatttatc
    45121 gcagcggtaa aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacgtatc
    45181 cggaagtgaa agcttttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat
    45241 gactaaacag cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct
    45301 gggggccggt ttaatccgcg ctctgaaaga acatgtgccc aacgcccgct ttgttggcgt
    45361 tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc
    45421 ggtgatgggc attgttgaag tgctcggtcg tctgcgtcgc ttactgcata ttcgtgccga
    45481 tctgacaaag cgttttggtg aactaaagcc agatgttttt gttggcattg atgcgcctga
    45541 cttcaatatt accctcgaag gtaaccttaa aaagcagggt atcaaaacca ttcattacgt
    45601 cagtccatcc gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga
    45661 tctggtgctc gcatttctgc ctttcgaaaa agcgttttat gacaaataca atgtaccgtg
    45721 ccgctttatc ggtcatacca tggctgatgc catgccgtta gatccagata aaaatgccgc
    45781 ccgtgatgtg ctggggatcc ctcacgatgc ccactgcctg gcgttgctac cggggagccg
    45841 tggcgcagaa gttgaaatgc ttagtgccga tttcctgaaa acggcccagc ttttgcgcca
    45901 gacatatccg gatctcgaaa tcgtggtgcc actggtgaat gccaaacgcc gcgagcagtt
    45961 tgaacgcatc aaagctgaag tcgcgccaga cctttcagtt catttgctgg atgggatggg
    46021 ccgtgaggcg atggtcgcca gcgatgcggc gctactggcg tcgggtacgg cagccctgga
    46081 gtgtatgctg gcgaaatgcc cgatggtggt gggatatcgc atgaagcctt ttaccttctg
    46141 gttggcgaag cggctggtga aaactgatta tgtctcgctg ccaaatctgc tggcgggcag
    46201 agagttagtc aaagaattat tgcaggaaga gtgtgagccg caaaaactgg ctgcggcgct
    46261 gttaccgctg ttggcgaacg ggaaaaccag ccacgcgatg cacgatacct tccgtgaact
    46321 gcatcagcag atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc
    46381 acaatgatcg aatttgttta tccgcacacg cagctggttg cgggtgtgga tgaagtcgga
    46441 cgcgggccgt tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc ggcgcgcccg
    46501 attgccgggc tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctatgaa
    46561 gagatcaaag agaaagcgtt gagctggagt ctgggccgcg cggaacccca cgaaatcgac
    46621 gagctgaaca ttcttcatgc gaccatgctg gcgatgcagc gtgccgtcgc tgggctgcat
    46681 attgcgccgg aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgcctgcg
    46741 atggctgtgg tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg
    46801 aaagtgacgc gtgacgccga aatggcggcg ctggatattg ttttcccgca atatggtttt
    46861 gcccaacaca aagggtaccc aaccgctttt catctggaaa aactggctga acacggcgcg
    46921 accgaacacc atcggcgcag ctttgggcct gtcaaacgcg cactgggact tgcgtcctga
    46981 ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac
    47041 ctgcgggtgc acagcgacta ctcgatgatc gatggcctgg ccaaaaccgc accgttggta
    47101 aaaaaggcgg cggcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgt
    47161 ggtctggtga agttctacgg agcgggacat ggcgcaggga ttaagcctat cgtcggggca
    47221 gattttaacg tccagtgcga cctgctgggt gatgagttaa cccacctgac ggtactggcg
    47281 gcgaacaata ccggctatca gaatctgacg ttgctgatct caaaagcgta tcagcgcggg
    47341 tacggtgccg ccgggccgat catcgatcgc gactggctta tcgaattaaa cgaagggttg
    47401 atccttcttt ccggcggacg catgggcgac gtcggacgca gtcttttgcg tggtaacagc
    47461 gcgctggtag atgagtgtgt cgcgttttat gaagaacact tcccggatcg ctattttctc
    47521 gagctgatcc gcaccggcag gccggatgaa gaaagctatc tgcacgcggc ggtggaactg
    47581 gcggaagcgc gcggtttgcc cgtcgtggcg accaacgacg tgcgctttat cgacagcagc
    47641 gactttgacg cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct
    47701 aaacgcccgc gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag
    47761 ctgtttgccg acatccccga agcccttgcc aacaccgttg agatcgccaa acgctgtaac
    47821 gtaaccgtgc gtcttggtga atacttcctg ccgcagttcc cgaccgggga catgagcacc
    47881 gaagattatc tggtcaagcg tgcaaaagag ggcctggaag agcgtctggc ctttttattc
    47941 cctgacgagg aagaacgtgt taagcgccgc ccggaatatg acgaacgtct ggagactgaa
    48001 cttcaggtta tcaaccagat gggcttcccg ggctacttcc tcatcgttat ggaatttatc
    48061 cagtggtcga aagataacgg cgtaccggta gggccaggcc gtggctccgg tgcgggttca
    48121 ctggtggcct acgcgctgaa aatcaccgac ctcgatccgc tggaatttga cctgctgttc
    48181 gaacgtttcc ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg
    48241 gagaaacgcg atcaggttat cgagcacgta gcggacatgt acggtcgtga tgcggtatcg
    48301 cagatcatca ccttcggtac aatggcggcg aaagcggtga tccgcgacgt aggccgcgtg
    48361 ctggggcatc cgtacggctt tgtcgatcgt atctcgaaac tgatcccgcc cgatccgggg
    48421 atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat
    48481 gaagaagtta aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc
    48541 ggtaagcacg ccggtggggt ggttatcgca ccgaccaaaa ttaccgattt tgcgccgctt
    48601 tattgcgatg aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac
    48661 gccggactgg tgaagttcga cttccttggt ttgcgtacgc tcaccatcat caactgggcg
    48721 ctggagatga tcaacaagcg gcgggcgaag aatggcgagc cgccgctgga tatcgccgcg
    48781 atcccgctgg atgacaagaa aagcttcgac atgctgcaac gctcggaaac cactgcggta
    48841 ttccagcttg aatcgcgcgg catgaaggac ctgatcaagc gtctgcaacc tgactgcttc
    48901 gaagatatga tcgccctggt ggcactgttc cgccccggtc cgttgcaatc agggatggtg
    48961 gataacttta tcgaccgtaa acatggtcgt gaagagatct cctatccgga cgtgcagtgg
    49021 cagcatgaaa gcttgaaacc ggtactggag ccaacctacg gcatcatcct gtatcaggaa
    49081 caggtcatgc agattgccca ggtgctttct ggttataccc tcggtggcgc ggatatgctg
    49141 cgtcgtgcga tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct
    49201 gaaggtgcag aaaagaacgg aatcaacgcc gaactggcga tgaaaatctt cgacctggtg
    49261 gagaaattcg cgggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca
    49321 tatcaaacgt tatggctgaa agcacactat cctgcggagt ttatggcggc ggtaatgacc
    49381 gccgatatgg acaacaccga gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg
    49441 ctgaaaatcc tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgac
    49501 ggcgaaatcg tgtatggtat tggcgccatc aaaggggtag gtgaaggtcc gattgaggcc
    49561 atcatcgaag cccgtaataa aggcggctac ttccgcgaac tgtttgatct ttgcgcccgt
    49621 accgacacca aaaagttaaa ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt
    49681 gaccgtcttg gaccacaccg cgcggcactg atgaactcgc tgggcgatgc gttaaaagcg
    49741 gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc
    49801 gaagagccgg aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg
    49861 gtattagatg gggaacgtga aacgttaggc ctgtacctga ccggacaccc tatcaaccag
    49921 tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca
    49981 gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc
    50041 aagcgcggca atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg
    50101 atgttgttta ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt
    50161 atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc
    50221 gaagtgatgg atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg
    50281 acggacaggc aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac
    50341 cgctctggga caattccagt acatctctac tatcagaggg cggatgcacg cgcgcggttg
    50401 cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc
    50461 ctcattggtt cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg
    50521 aatttccttg attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgact
    50581 gcggttagcc gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtctg
    50641 cgtgaaaaaa gcgtagaact gacgcgtaaa atcttcgccg atctcggtgc atggcagatt
    50701 gcgcaactgg cacgccatcc acagcgtcct tataccctgg attacgttcg cctggcattt
    50761 gatgaatttg acgaactggc tggtgaccgt gcgtatgcag acgataaagc tatcgtcggt
    50821 ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa
    50881 accaaagaaa aaattcgccg taactttggt atgccagcgc cagaaggtta ccgcaaagca
    50941 ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta tcatcacctt tatcgacacc
    51001 ccgggggctt atcctggcgt gggcgcagaa gagcgtggtc agtctgaagc cattgcacgc
    51061 aacctgcgtg aaatgtctcg cctcggcgta ccggtagttt gtacggttat cggtgaaggt
    51121 ggttctggcg gtgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc
    51181 acctattccg ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgccgacaaa
    51241 gctccgctgg cggctgaagc gatgggtatc atcgctccgc gtctgaaaga gctgaaactg
    51301 atcgactcca tcatcccgga accgctgggc ggcgctcacc gtaacccgga agcgatggcg
    51361 gcatcgttga aagcgcaact gctggcggat ctggccgatc tcgatgtgtt aagcactgaa
    51421 gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg gttacgcgta attcgcaaaa
    51481 gttctgaaaa agggtcactt cggtggccct tttttatcgc cacggtttga gcaggctatg
    51541 attaaggaag gattttccag gaggaacaca tgaacatcat tgccattatg ggaccgcatg
    51601 gcgtctttta taaagatgag cccatcaaag aactggagtc ggcgctggtg gcgcaaggct
    51661 ttcagattat ctggccacaa aacagcgttg atttgctgaa atttatcgaa cataaccctc
    51721 gaatttgcgg cgtgattttt gactgggatg agtacagtct cgatttatgc agcgatatca
    51781 atcaacttaa tgaatatctc ccgctttatg ccttcatcaa cacccactcg acgatggatg
    51841 tcagcgtgca ggatatgcgg atggcgctct ggttttttga atatgcgctg gggcaggcgg
    51901 aagatatcgc cattcgtatg cgtcagtaca ccgacgaata tcttgataac attacaccgc
    51961 cgttcacgaa agccttgttt acctacgtta aagagcggaa gtacaccttt tgtacgccgg
    52021 ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg ctgcctgttt tatgattttt
    52081 tcggcgggaa taccctcaag gctgatgtct ctatttcggt caccgagctt ggttcgttgc
    52141 tcgaccacac cgggccacac ctggaagcgg aagagtacat cgcgcggact tttggcgcgg
    52201 aacagagtta tatcgttacc aacggaacat cgacgtcgaa caaaattgtg ggtatgtacg
    52261 ccgcgccatc cggcagtacg ctgttgatcg accgcaattg tcataaatcg ctggcgcatc
    52321 tgttgatgat gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga
    52381 ttcttggtgg gatcccgcgc cgtgaattta ctcgcgacag catcgaagag aaagtcgctg
    52441 ccaccacgca ggcacaatgg ccggttcatg cggtgattac caactccacc tatgatggct
    52501 tgctctacaa caccgactgg atcaaacaga cgctggatgt cccgtcgatc cacttcgatt
    52561 ccgcctgggt gccgtacacc cattttcatc cgatctacca ggggaaaagt ggtatgagcg
    52621 gcgagcgtgt tgcgggaaaa gtgatcttcg aaacgcaatc gacccacaaa atgctggcgg
    52681 cgttatcgca ggcttcgctg atccacatta aaggcgagta tgacgaagag gcgtttaacg
    52741 aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct tcggttgaga
    52801 cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt tcggtggagc
    52861 gggcgctgca ttttcgcaaa gaggtccagc ggttgcggga agagtctgac ggttggtttt
    52921 tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcccgtt gcgccaggcg
    52981 aacagtggca cggctttaac gatgcggatg ccgatcatat gtttctcgat ccggttaaag
    53041 tcactatttt gacaccgggg atggacgaac agggcaatat gagcgaggag gggatcccgg
    53101 cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa accggccctt
    53161 ataacctgct gtttctcttt agtattggta tcgataaaac caaagcaatg ggattattgc
    53221 gtgggttgac ggaatttaaa cgctcttacg atctcaacct gcggatcaaa aatatgctac
    53281 ccgatctcta tgctgaagat cccgatttct accgcaatat gcgtattcag gatctggcgc
    53341 aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg cgggcattcg
    53401 atactttgcc ggaaatgatc atgacgccac atcaggcatg gcagagacag attaaaggtg
    53461 aagtagaaac cattgcgctg gaacaactgg tcggtagagt atcggcaaat atgatcctgc
    53521 cttatccacc gggcgtaccg ctgttgatgc caggagaaat gctgaccaaa gagagccgca
    53581 cggtactcga ttttctactg atgctttgtt ccgtagggca acattacccc ggttttgaaa
    53641 cggatattca cggcgcgaaa caggacgaag acggcgttta ccgcgtacga gtcctaaaaa
    53701 tggcgggata acttgccaga gcggcttccg ggcgagtaac gtgctgttaa caaataaagg
    53761 agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga cggattatgc
    53821 ggtgagcaaa gctttctact gcgatattct tggtttcacg ctgcaaagcg aagtctatcg
    53881 cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg tgattgagct
    53941 tttctctttt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca
    54001 tctggctttt agcgttgatg acatcgatgc ggcagtggcg caccttgaaa gccataacgt
    54061 gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa cgcttcacct tctttaacga
    54121 tccggacggg ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc
    54181 cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat catgacactc acgctcaata
    54241 gacaacttct cacctcacgc cagattctgg tggcctttag cggcgggctt gactccaccg
    54301 ttctgctgca tcagttggtg cagtggcgga cggaaaatcc gggcgtcact ctgcgcgcta
    54361 ttcatgtgca tcacggttta agtgccaatg ccgatgcctg ggttacgcat tgcgaaaacg
    54421 tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt acaacttgcg caagaggggc
    54481 tggggattga ggcccaagcg cggcaggcgc gttatcaggc atttgcccgt accctattgc
    54541 ccggcgaagt gctggtcacc gcgcaacatc tcgacgatca atgtgaaacc tttctgctgg
    54601 cgctaaaacg cggtagcggc cctgccgggc tttcggctat gggggaagtc tcggagtttg
    54661 ccggaacacg gcttatccgc ccgttgcttg cccgcacgcg gggggaactg gcgcagtggg
    54721 cattggcgca tggtttacgc tggattgaag acgaaagtaa tcaggacgac tcatacgatc
    54781 gtaactttct gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg cattttgccg
    54841 aagcaacggc ccgcagcgcc gcactttgtg ctgaacaaga gagtctgttg gatgaactgc
    54901 tggcggatga tttagcacac tgccaaacgt cgcaggggac gctgcagatt gcgccaatgc
    54961 tagcgatgag tgatgcccgt cgcgcggcga ttatccgccg ctggctggca gggcagaatg
    55021 caccgatgcc ttcccgcgac gcgctggtgc ggatctggca ggaagtggcg ctggcgcggg
    55081 aagatgcctc cccctgttta cgttttggcg cgtttgaaat acggcgctat cagtcgcaac
    55141 tgtggtggat taactccgtc actgggcaaa gcgaaaccat tgtgccgtgg cagacgtggc
    55201 tacaaccgct ggaattaccg gcggggctgg gaagtgtaca gcttactgcg ggaggcgata
    55261 ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaagcgcca ggattgctgc
    55321 atattgtcgg gcgtaacggc ggacgtaagc taaagaaaat ctggcaagag ctgggcgtgc
    55381 cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgctg attgcggcgg
    55441 caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgta agttttgtct
    55501 ggcagaaaac gcttagttaa gtgaaagccg gataagacgc atcaaacgtc gcatccggcg
    55561 aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct aaagctggta
    55621 attttatcca gacgtagctc acgggtttcg ccagcggcct cgacgaccag atactccaca
    55681 tttttgcggg agactaaatc gctggctttt gcctgcaatt tttcgccatc tttcagctca
    55741 agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc atcacaattg
    55801 attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg ccgcagcgta
    55861 tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt cattcagcac
    55921 tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg cgatttccgc
    55981 gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca gcactctgac
    56041 ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa ggtgactata
    56101 ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac aatggcctcc
    56161 tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc gacggttaat
    56221 gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc gcgccaccct
    56281 tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt ggctgaaata tgctgatgaa
    56341 cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac
    56401 gatcacctgg gcattgattt gtggattgag ctggggctgc cggatgagcg gcggattaag
    56461 aaagcctgca cccaggccgc agaagtggtg ctgtttacct ataatagtcg ggcggcgcaa
    56521 atctggtggc agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc cgtctggtat
    56581 ctggacgatg aacaactggc gaaagtaagc gcctttgccg atcgtaccat gacgctgcag
    56641 gcaacgattc aggatggcgt gatctggtta tcggacgata agaataatct ggaagttaac
    56701 ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg acatgttgct attcccgatg
    56761 gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat gttaataaga
    56821 cctcaacggc cattcatctg cgttttgaca ttcgggcgtc cagcctgcca gagtattaca
    56881 aagagcgtct actcgccgcc agccatcatt tgatcagcag tgatggcgtg attgtcatta
    56941 aggcacagga ataccgcagt caggaactga accgcgaagc cgcgctagcc cggctggtgg
    57001 cagtgattaa agatttaaca acagaacaaa aagcccgacg acccacgcgg cccacccgtg
    57061 catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg
    57121 gcaaagtacg cagcggtcgg gaataaaaag aaggaataga tggtgaaaaa agcgatagtg
    57181 acagcgatgg ctgtaatcag cctctttact ctgatgggat gtaataatcg ggccgaagtt
    57241 gatgcgcttt ctccggcgca ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc
    57301 gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc tgttcctcga aaaagacgga
    57361 acatgggtga tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc
    57421 tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa aggtgaaaag
    57481 tcatattatc gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg caatccgatt
    57541 gaatcgcagt tcaactatac gctggaaccg gcacaatcca gtttacctat gacgccgatg
    57601 accctgcgag gcatgtattt ttatatggct gatgcggcga ccttcactga ttgcgcgacc
    57661 ggaaaacgtt tcatggtagc gaataacgca gagctggagc gtagctacct ggctgcgcgc
    57721 ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc actttacgct tgaggctaat
    57781 ccggatactg gtgcgccaac caaagtatta gcgcccgata cggcgggtaa attttacccg
    57841 aataaggatt gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca aacgcaaaat
    57901 tgcctgatgc gct
//
LOCUS       DAPHDX010000009        54112 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPHDX010000009 DAPHDX010000000
VERSION     DAPHDX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.009"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            281..1375
                     /gene="gcvT"
                     /locus_tag="RH058_000556"
     CDS             281..1375
                     /gene="gcvT"
                     /locus_tag="RH058_000556"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV3136195.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1399..1788
                     /gene="gcvH"
                     /locus_tag="RH058_000557"
     CDS             1399..1788
                     /gene="gcvH"
                     /locus_tag="RH058_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV3136196.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYETLLEDE"
     gene            1907..4780
                     /gene="gcvP"
                     /locus_tag="RH058_000558"
     CDS             1907..4780
                     /gene="gcvP"
                     /locus_tag="RH058_000558"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV3136197.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5046..5789
                     /locus_tag="RH058_000559"
     CDS             5046..5789
                     /locus_tag="RH058_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3136198.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5846..7279)
                     /gene="bglA"
                     /locus_tag="RH058_000560"
     CDS             complement(5846..7279)
                     /gene="bglA"
                     /locus_tag="RH058_000560"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV3136199.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7324..7635
                     /locus_tag="RH058_000561"
     CDS             7324..7635
                     /locus_tag="RH058_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3136200.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7799..8458
                     /locus_tag="RH058_000562"
     CDS             7799..8458
                     /locus_tag="RH058_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV3136201.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8535..9515)
                     /gene="ygfZ"
                     /locus_tag="RH058_000563"
     CDS             complement(8535..9515)
                     /gene="ygfZ"
                     /locus_tag="RH058_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV3136202.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9758..10024
                     /gene="sdhE"
                     /locus_tag="RH058_000564"
     CDS             9758..10024
                     /gene="sdhE"
                     /locus_tag="RH058_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV3136203.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10005..10412
                     /locus_tag="RH058_000565"
     CDS             10005..10412
                     /locus_tag="RH058_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDV3136204.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10452..10973)
                     /gene="fldB"
                     /locus_tag="RH058_000566"
     CDS             complement(10452..10973)
                     /gene="fldB"
                     /locus_tag="RH058_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV3136205.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11085..11981
                     /gene="xerD"
                     /locus_tag="RH058_000567"
     CDS             11085..11981
                     /gene="xerD"
                     /locus_tag="RH058_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV3136206.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12006..12716
                     /gene="dsbC"
                     /locus_tag="RH058_000568"
     CDS             12006..12716
                     /gene="dsbC"
                     /locus_tag="RH058_000568"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV3136207.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12722..14455
                     /gene="recJ"
                     /locus_tag="RH058_000569"
     CDS             12722..14455
                     /gene="recJ"
                     /locus_tag="RH058_000569"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV3136208.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLCDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14546..15644
                     /gene="prfB"
                     /locus_tag="RH058_000570"
     CDS             join(14546..14620,14622..15644)
                     /gene="prfB"
                     /locus_tag="RH058_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV3136209.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15654..17171
                     /gene="lysS"
                     /locus_tag="RH058_000571"
     CDS             15654..17171
                     /gene="lysS"
                     /locus_tag="RH058_000571"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV3136210.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17214..17762)
                     /gene="idi"
                     /locus_tag="RH058_000572"
     CDS             complement(17214..17762)
                     /gene="idi"
                     /locus_tag="RH058_000572"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV3136211.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17817..17888
                     /locus_tag="RH058_000573"
     CDS             17817..17888
                     /locus_tag="RH058_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136212.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17885..18010)
                     /locus_tag="RH058_000574"
     CDS             complement(17885..18010)
                     /locus_tag="RH058_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136213.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18012..19460)
                     /gene="uacT"
                     /locus_tag="RH058_000575"
     CDS             complement(18012..19460)
                     /gene="uacT"
                     /locus_tag="RH058_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV3136214.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19896..21815
                     /gene="ygfT"
                     /locus_tag="RH058_000576"
     CDS             19896..21815
                     /gene="ygfT"
                     /locus_tag="RH058_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV3136215.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21815..22303
                     /locus_tag="RH058_000577"
     CDS             21815..22303
                     /locus_tag="RH058_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV3136216.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22339..23706)
                     /gene="ghxQ"
                     /locus_tag="RH058_000578"
                     /gene_synonym="ygfQ"
     CDS             complement(22339..23706)
                     /gene="ghxQ"
                     /locus_tag="RH058_000578"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV3136217.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23742..25058)
                     /gene="guaD"
                     /locus_tag="RH058_000579"
     CDS             complement(23742..25058)
                     /gene="guaD"
                     /locus_tag="RH058_000579"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV3136218.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25076..26476)
                     /gene="xanQ"
                     /locus_tag="RH058_000580"
     CDS             complement(25076..26476)
                     /gene="xanQ"
                     /locus_tag="RH058_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV3136219.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26641..29511)
                     /locus_tag="RH058_000581"
     CDS             complement(26641..29511)
                     /locus_tag="RH058_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV3136220.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGVPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29508..30287)
                     /locus_tag="RH058_000582"
     CDS             complement(29508..30287)
                     /locus_tag="RH058_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV3136221.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYISGVLVADLYADCQQAEEEAV"
     gene            complement(30338..31666)
                     /gene="ssnA"
                     /locus_tag="RH058_000583"
     CDS             complement(30338..31666)
                     /gene="ssnA"
                     /locus_tag="RH058_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV3136222.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31669..34767)
                     /gene="ygfK"
                     /locus_tag="RH058_000584"
     CDS             complement(31669..34767)
                     /gene="ygfK"
                     /locus_tag="RH058_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV3136223.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSLVLNIDSEGQFDNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(35089..35667)
                     /gene="mocA"
                     /locus_tag="RH058_000585"
     CDS             complement(35089..35667)
                     /gene="mocA"
                     /locus_tag="RH058_000585"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV3136224.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            35770..36540
                     /gene="yqeC"
                     /locus_tag="RH058_000586"
     CDS             35770..36540
                     /gene="yqeC"
                     /locus_tag="RH058_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV3136225.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36588..38213
                     /locus_tag="RH058_000587"
     CDS             36588..38213
                     /locus_tag="RH058_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV3136226.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38254..39186)
                     /gene="arcC"
                     /locus_tag="RH058_000588"
     CDS             complement(38254..39186)
                     /gene="arcC"
                     /locus_tag="RH058_000588"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV3136227.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERLIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39234..40619)
                     /gene="hyuA"
                     /locus_tag="RH058_000589"
     CDS             complement(39234..40619)
                     /gene="hyuA"
                     /locus_tag="RH058_000589"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV3136228.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40672..41883)
                     /locus_tag="RH058_000590"
     CDS             complement(40672..41883)
                     /locus_tag="RH058_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV3136229.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41941..43137)
                     /gene="dpaL"
                     /locus_tag="RH058_000591"
     CDS             complement(41941..43137)
                     /gene="dpaL"
                     /locus_tag="RH058_000591"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV3136230.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43195..44382)
                     /gene="ygeW"
                     /locus_tag="RH058_000592"
     CDS             complement(43195..44382)
                     /gene="ygeW"
                     /locus_tag="RH058_000592"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV3136231.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            44861..46639
                     /locus_tag="RH058_000593"
     CDS             44861..46639
                     /locus_tag="RH058_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV3136232.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(46679..47158)
                     /gene="xdhC"
                     /locus_tag="RH058_000594"
     CDS             complement(46679..47158)
                     /gene="xdhC"
                     /locus_tag="RH058_000594"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV3136233.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(47155..48033)
                     /gene="xdhB"
                     /locus_tag="RH058_000595"
     CDS             complement(47155..48033)
                     /gene="xdhB"
                     /locus_tag="RH058_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV3136234.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(48044..50341)
                     /gene="xdhA"
                     /locus_tag="RH058_000596"
     CDS             complement(48044..50341)
                     /gene="xdhA"
                     /locus_tag="RH058_000596"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV3136235.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            50756..51511
                     /locus_tag="RH058_000597"
     CDS             50756..51511
                     /locus_tag="RH058_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV3136236.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            51590..51663
                     /locus_tag="RH058_000598"
     tRNA            51590..51663
                     /locus_tag="RH058_000598"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51622..51624,aa:Gly,seq:ccc)
     gene            51897..53507
                     /locus_tag="RH058_000599"
     CDS             51897..53507
                     /locus_tag="RH058_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136237.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMKDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            53520..53639
                     /locus_tag="RH058_000600"
                     /pseudo
     CDS             53520..53639
                     /locus_tag="RH058_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            53639..54004
                     /locus_tag="RH058_000601"
     CDS             53639..54004
                     /locus_tag="RH058_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136238.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     HVGKVFDGVLSPAQPLIRI"
BASE COUNT        13412 a        13353 c        14111 g        13236 t
ORIGIN      
        1 tcttaatatt gccttattca aattagcttt ctaatggcat catctttata tagaaactgg
       61 tgtgtttttt ggttttttat tctgtcgcga ttttggcgtt ttttaaccat aagctaatgt
      121 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
      181 aacgattgcg ttggggaccg ggagtggctc cgatgctggg tttcgtggtg ataatttcac
      241 catgaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac agactccttt
      301 gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacggct ggatgatgcc
      361 gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg ccggaatgtt
      421 tgatgtgtca catatgacca tcgtcgatct ccgcggcagc cgcacccggg agtttctgcg
      481 ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc tttactcggg
      541 gatgttgaat gcctctggcg gtgtgataga tgacctcatc gtctactact ttactgaaga
      601 tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct ggattaccca
      661 acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca tgattgccgt
      721 gcaagggccg aatgcgcagg caaaagctgc cacactgttt aatgacgccc agcgtcaggc
      781 ggtggaaggg atgaaaccgt tctttggcgt gcaggcgggc gatctgttta ttgccaccac
      841 tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag cggccgattt
      901 ctggcgtgcg ctggtggaag cgggtgttaa gccatgtggc ttgggcgcgc gtgacacgct
      961 gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta tttctccttt
     1021 agccgccaac atgggctgga ctatcgcctg ggaaccggca gatcgtgact ttatcggtcg
     1081 tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc tggtgatgac
     1141 cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc agggcaacca
     1201 gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca gcattgcgct
     1261 ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca accgtgaaat
     1321 gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg cgtgatttac
     1381 ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag caaagaacac
     1441 gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtataaccga acatgcccag
     1501 gagctgttag gcgatatggt gtttgttgac ctgccggaag tgggcgcaac ggttagcgcg
     1561 ggcgatgact gcgcggttgc cgaatcagta aaagcggcgt cagacattta tgcgccagta
     1621 agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact ggtgaacagc
     1681 gaaccgtatg caggtggctg gatctttaaa atcaaagcca gcgatgaaag cgaactggaa
     1741 tcactgctgg atgcgaccgc atacgaaaca ttgttagaag acgagtaacg gctttattcc
     1801 tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac tctcttcgta
     1861 aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga cacagacgtt
     1921 aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg acgccgcgca
     1981 acagcaagaa atgctgaatg ccgttggtgc acaatcgtta aacgcgctga ccggccagat
     2041 tgtgccgaaa gatattcagc ttgcgacacc accgcaggtt ggcgcaccgg cgaccgaata
     2101 cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca cgtcttacat
     2161 cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca tgctggaaaa
     2221 tccgggctgg tataccgcgt acactccgta tcaacctgaa gtctcccagg gccgccttga
     2281 agcactgctc aacttccagc aggtaacgct ggatttgact ggactggata tggcctctgc
     2341 ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg gcgatggcga aacgcgtcag
     2401 caaactgaaa aatgccaacc gcttcttcgt ggcttccgat gtgcatccgc aaacgctgga
     2461 tgtggtccgt actcgtgccg aaacctttgg ttttgaagtg attgtcgatg acgcgcaaaa
     2521 agtgctcgac catcaggacg tcttcggcgt gctgttacag caggtaggca ctaccggtga
     2581 aattcacgac tacactgcgc ttattagcga actgaaatca cgcaaaattg tggtcagcgt
     2641 tgccgccgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg gcgcggatat
     2701 tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc tacggtggcc cacacgcggc
     2761 attctttgcg gcgaaagatg aatacaaacg ctcaatgccg ggccgtatta tcggtgtatc
     2821 gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg agcaacatat
     2881 tcgtcgtgag aaagcgaact ccaacatttg tacttcccag gtactgctgg caaacatcgc
     2941 cagcctgtat gccgtttatc acggcccggt tggcctgaaa cgtatcgcta accgcattca
     3001 ccgtctgacc gatattctgg cggcgggcct gcaacaaaaa ggtctgaaac tgcgccatgc
     3061 gcactatttc gacaccttgt gtgtggaagt ggccgacaaa gcgggcgtac tgacgcgtgc
     3121 cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gccgtgggga tcacccttga
     3181 tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagc gtgctgctgg gcgataacca
     3241 cgggctggag atcgacacgc tggacaaaga cgtggctcac gacagccgct ctatccagcc
     3301 tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg tttaatcgct accacagcga
     3361 aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc tgaatcaggc
     3421 gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga tgatccccat
     3481 cacctggccg gaatttgccg aactgcaccc gttctgcccg ccggaacagg ccgaaggtta
     3541 tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt acgacgccgt
     3601 ttgtatgcag ccgaactctg gcgcacaggg cgaatacgcg ggcctgctgg cgattcgtca
     3661 ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatcccgg cttctgcgca
     3721 cggaactaac cccgcttctg cacatatggc aggaatgcag gtggtggttg tggcgtgtga
     3781 taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa gcggaacagg cgggcgataa
     3841 cctctcctgt atcatggtga cttatccttc tacccacggc gtgtatgaag aaacgatccg
     3901 tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg gcgcgaacat
     3961 gaacgcccag gttggcatca cctcgccggg ctttattggc gcggacgttt cgcacctcaa
     4021 cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg gaccgatcgg
     4081 tgtgaaagcg catctggcac cgtttgtacc gggtcatagc gtggtgcaaa tcgaaggcat
     4141 gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta tcctgccaat
     4201 cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa gccaggtggc
     4261 aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg tgctgtatac
     4321 cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc tgaaagaaga
     4381 aaccggcatc agcgagctgg atattgccaa gcgcctgatc gactacggtt tccacgcgcc
     4441 gaccatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat ctgaaagcaa
     4501 agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa tcgaccaggt
     4561 taaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccgc acattcagaa
     4621 cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcagtat tcccggcagg
     4681 tgtggcagac aaatactggc cgacggtgaa acgtctggat gatgtttacg gcgaccgtaa
     4741 cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt cgactatctt
     4801 ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt acaaaagcgc tcagacagtc
     4861 ccctcacccc tttggggaga gggttagggt gaggggaaca ggccggcact ggcgcgaata
     4921 tttaccctca ccccggccct ctccctgaaa gggcgagggg aaaagcgtgc caacattgaa
     4981 gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt ttctcggaaa
     5041 aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg gcaactgcat
     5101 tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac ctccacgcgg
     5161 cgcaggaagt gatgaactta ataacgcaag ccggtggcaa agcattcgtg ctccaggcgg
     5221 atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag cacgatgaac
     5281 cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc gttgaaaacc
     5341 ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat tttctctgct
     5401 gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc gctatcgtca
     5461 atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat tacgcggcat
     5521 cgaaaggggc gattgatacg ttaaccaccg gactatcgct ggaagtcgcc gcgcagggga
     5581 tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc agcggcggcg
     5641 agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt ggacaggcag
     5701 aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac gtcacgggaa
     5761 gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg gatgcaacaa
     5821 gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca atcacctctt
     5881 ggtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagcaccg tcgtcatgtt
     5941 tattcacata gataaagccg tagcgtttgc tgtactgccc ggtggtgaac gatacgcagt
     6001 cgatgcagcc ccacggtgtg tagcccatca gatccacgcc atcgtaagtc accgcttttt
     6061 tcatctcttc gatatgggcg cgcaggtagt caatgcggta gtcgtcgttg atgctgccat
     6121 cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc ggcctctgat
     6181 aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc tgccagcccc
     6241 agtcagacgc tttaacatac gggttcggta cgctgccttc gaaaccagag atcgcatcgc
     6301 cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca agataatcgc
     6361 aggtgccttc acgcagtaca tcgagatcgc cgtcttccat tttgatgtta aatccgcgac
     6421 gctcccactc gttcaacaca taggacgggt aatagccgcg tagctgcaca tcggtaaaga
     6481 cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta cagcagtagg
     6541 gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta atgcgacgcg
     6601 cagctttcac cgccagggcg ctggcgacaa actggtgatg cagcacctga tacatcgtct
     6661 cttccgggtt ctcatgctcg gtataaacca cgccggagca gcagtaaccg aacagcggtg
     6721 cacgccagtt acgctggttg ttaatttcgt tgaaggtcat ccagtatttg actttatgct
     6781 tatagcgttc aaacaccact tccgcgaaac ggacaaagaa atcaaccact ttacggttgg
     6841 tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag agggtgatca
     6901 ccggttcgat gttgtatttc agcagttcat cgaacatatc atcgtagaac ttcagccctt
     6961 cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg atggaagtcc
     7021 gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag tgaccataaa
     7081 aatcaacggc ttcatggttt ggatagtatt tgcctggcaa gacttctttg gtgatttcgc
     7141 gcggcacgcc gtgtgcgcca ccggtcagaa cgtcacaaat gctcggccct ttgccgcctt
     7201 tgttccagcc gccttcgacc tgatgagcgg caactgcgcc gccccataag aaatctttcg
     7261 gtaaggtgag ttttttcact atcataggct ccagatgagt caattcttac aggaaatagt
     7321 accatgcagc caaacgacat cacttttttt caacgtttcc aggatgacat tctggctggg
     7381 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
     7441 cgtgtcgggc gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
     7501 accgtaacgc tggatacgct gacggaaaaa catgcagagc aggaaaatat gaccctgact
     7561 gaactgataa aggtcattgc cgacatctat cccggtcaga cacagtttta tgtgattgaa
     7621 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
     7681 atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
     7741 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctccgg agtaagttat
     7801 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
     7861 cagtcacggc attgggttgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
     7921 ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg gcggcagtat
     7981 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
     8041 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
     8101 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
     8161 cctggcattg ctgggtattc tgtttaaact gaccatcgcg caccgattca aaattttatc
     8221 tctggtgacc tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
     8281 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
     8341 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
     8401 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatt
     8461 gctgtgcctt tgtctgcatg aaaagacaca tcagtgtcgc ttcaggcaat agatgcccga
     8521 agcgatatag aaatttactc ttcgagcgaa tacggcagcg gctcgatatg cagtgtattt
     8581 gcatcgtcgc gcacgcggaa gatgctgtcc ggttccatat cgttattcat caccacctgc
     8641 accacgacct gaccatcttc cagtttgacc gcagccagca ccgtaccggt acgacgccag
     8701 ttttcgccca ttttcagctc taagtcttca cctgcttctg gcagtcggct ggcgctacct
     8761 gccagtaacc agagcgcacg tttgttggca ccgcggaatt tggcccgcgc caccatctct
     8821 tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag attggtcgcc
     8881 tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaacccgc ttcaatgttt
     8941 aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc
     9001 atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat
     9061 agcaaagtgg tcgcgccttc tttgactacc tgtttttctt tcgaaggcag ttcgctaaag
     9121 agatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg
     9181 tctggcgcga tggtcacttt agagaatacc gcatattttt tcagttcagt cagctgcggt
     9241 tcacgcacgc tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa cagacgtaaa
     9301 ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg atcttctgcc
     9361 atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta
     9421 atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc
     9481 gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg
     9541 cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc actacgcgat
     9601 ctgtgccttc tgcgaggcga cttccaaaaa aatgaagcca ttgaattgtt gtgaattaat
     9661 ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aaggcgaaaa acacgttaca
     9721 atagccgggt actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg
     9781 cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt
     9841 cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga
     9901 atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga
     9961 actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat
    10021 ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg
    10081 ccgctgttat tttactcatg ccctggccac tcagttacac cccgttatgg atggtgttac
    10141 tttcgctggt ggtgtttgat tgcgttcgca gccagcggcg tattaatgct cgccaggggg
    10201 aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg tgcatcgtca
    10261 aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat ggcggtaaac
    10321 ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg gatttacggc
    10381 ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata tgcgctaagc
    10441 cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc
    10501 tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac
    10561 agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag
    10621 tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg
    10681 aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct
    10741 tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcacca
    10801 aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag
    10861 tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct
    10921 gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa
    10981 gcttctcgat tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt agtcacggca
    11041 caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg
    11101 cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt
    11161 tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga
    11221 cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg
    11281 ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc
    11341 tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc
    11401 cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcaccat
    11461 taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg
    11521 ggctgcgtgt ctctgaactg gtcggactga caatgagtga tatcagcctg cgtcagggcg
    11581 tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt accgttaggt gaagaggcgg
    11641 tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat ggtgtgtcaa
    11701 ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc
    11761 gcattaaaca ttatgctgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtat
    11821 tgcgtcacgc ttttgccacc catttattaa atcatggtgc ggatttacgc gtggtgcaga
    11881 tgctactggg ccacagcgac ctctccacca cgcaaattta tacgcatgtc gctaccgagc
    11941 gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa
    12001 gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggttttgctc
    12061 aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata
    12121 ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggc gtgttgtaca
    12181 tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagc ggcacggctc
    12241 cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga
    12301 tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg
    12361 gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg atcaccgtgc
    12421 gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa atgaaagcta
    12481 tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg
    12541 caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg
    12601 ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc
    12661 cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg
    12721 cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc
    12781 tgaattgcct cccttgctgc gccgtttata cgccagccgg ggcgtgcgca gtgcgcaaga
    12841 actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctgagcggcg tcgaaaaggc
    12901 cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg tcggcgattt
    12961 tgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg
    13021 cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga
    13081 agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat
    13141 ttcctcccat gcgggggttg aacacgctcg ctcgttgggc attccggtta ttgttaccga
    13201 tcaccatttg ccaggcgaaa cattacccgc ggcggaagcg atcattaacc ctaacttgcg
    13261 cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct
    13321 ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgtggca tcgcaattcc
    13381 taacctggca gaactgctgg atctggtagc gctggggaca gtagcggacg tcgtgccgct
    13441 ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg ccggaaagtg
    13501 ccgtccgggg attaaagcgc tgctggaagt ggcaaatcgc gatgcgcaaa aactcgccgc
    13561 cagcgattta ggttttgcgc tggggccacg tctcaatgct gccgggcgac tggacgatat
    13621 gtccgtcggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa
    13681 tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc aagttgaagc
    13741 cctgaccctg tgcgagaaac tggagcgaag tcgcgacacg ctacccggcg ggctggcaat
    13801 gtaccacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg
    13861 ttttcaccgt ccggttatcg cctttgcgcc agcaggtgat ggtacgctga aaggttcagg
    13921 tcgctccatt caggggctgc atatgcgtga tgcactggag cgattagaca cactctaccc
    13981 tggcatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga
    14041 taaattcgaa ctctttcaac aacggtttgg cgaactggtt acagagtggc tggacccttc
    14101 gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgaccatgga
    14161 agtggcgcag ttgctgtgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt
    14221 tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaggtgat
    14281 ggtcgaaccg gtcggcggcg gtccactgct ggatggtatt gcttttaatg tcgataccgc
    14341 cctctggccg gataacggcg tgcgtgaagt gcaactggct tataagctcg atatcaacga
    14401 gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca
    14461 tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga
    14521 ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca
    14581 ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag
    14641 agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac
    14701 gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc
    14761 aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg
    14821 acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc
    14881 agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata
    14941 ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt
    15001 atctgcgctg ggctgaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg
    15061 aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc
    15121 tgcgtacaga aactggcgtt caccgcctgg tgcgtaaaag cccgtttgac tccggcggtc
    15181 gtcgccacac atcgttcagc tctgcgtttg tttacccgga agttgatgat gatattgata
    15241 tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc gcgggcggtc
    15301 agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccaacc gggatcgtga
    15361 cccaatgcca gaacgaccgt tcccagcaca agaacaaaga ccaggccatg aagcagatga
    15421 aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag gcgatggaag
    15481 ataacaaatc cgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc
    15541 gcattaaaga tctgcgtacc ggggtagaaa cccgcaacac gcaggccgtg ctggacggca
    15601 gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg
    15661 aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc
    15721 gtgagaagct ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat ttccgtcgcg
    15781 atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag
    15841 cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag
    15901 cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt gcccgtgacg
    15961 atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggt gacatcctcg
    16021 gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc
    16081 tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc
    16141 aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgtaaca
    16201 cctttaaagt gcgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct
    16261 ttatggaagt tgaaacgccg atgatgcagg tgatccctgg cggtgccgct gcgcgtccgt
    16321 ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt
    16381 acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc
    16441 gtaacgaagg tatctccgta cgtcataacc cagagttcac catgatggaa ctctacatgg
    16501 cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac
    16561 aggatattct cggtaagacg gaagtgacct atggcgacgt gacgctggac ttcggtaaac
    16621 cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg
    16681 cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt atccacgttg
    16741 agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac
    16801 atctgattca gccgaccttc attactgaat atccggcaga agtttctccg ctggcgcgtc
    16861 gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt ggtcgtgaaa
    16921 tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc ttcctggatc
    16981 aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa gattacgtta
    17041 ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc gaccgtatgg
    17101 taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc
    17161 cggtgaaata agcattacgt tatgctcaca accccggcaa atgtcggggt tttttattta
    17221 agctgggtaa atgcagataa tcgtattctg gcttcgcgat ttgtcgcctg catcaccatc
    17281 cacggactga acgcccacgg cgtggcatca ataccgtgta atacatcagc taaatcacac
    17341 cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca
    17401 aataccggac acacttcatt ttccacaatg ccactcggat cggtggcgcg gtagcgaaag
    17461 tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg
    17521 atcaccgcgt cttcgctgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac
    17581 acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg ccccttggca
    17641 ttaaacagcc aactggagaa cgcgagatgt aagagggtgt ctgccgtgtg tgcggcatac
    17701 ttttccagcg taccagtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc
    17761 ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga
    17821 ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga caggtaaaaa
    17881 ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt
    17941 cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta
    18001 aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata
    18061 cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag gtcagcatga
    18121 tgctggtagc cattaaagaa gacattcagc acaacggcgc taagggttgc gagcataatg
    18181 ccgctatgca gcaacggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc
    18241 gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg
    18301 gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcacc
    18361 agcccagcac cgcccagcac aaattgcgga atggaggcga ccagcaccgc catttttggc
    18421 accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg
    18481 cgcgtcacgc taaccaggcc aacgttttgt gaaaatgacg tgtgggggaa gctgttaaac
    18541 gttccgccta tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgtgaa
    18601 gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac
    18661 acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat cggcatacca
    18721 aatgacatcg gcgtgacaat cgcaaaccat gaagcatcat gtagcccgga taaattgact
    18781 tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg
    18841 gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac agcaaaggag
    18901 atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc ggcccagtca
    18961 atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt aaccagtggc
    19021 gggaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat aaaacccgcg
    19081 gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc
    19141 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    19201 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    19261 atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    19321 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    19381 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt
    19441 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg
    19501 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    19561 ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc acgaaaatga
    19621 gaagagaacc tgacaatgaa attatctgta attttctcat attgatattt cattaatcat
    19681 tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc ttttggtcgg
    19741 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    19801 aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat cgcgaaattt
    19861 gagaagacat aactcattaa atgaaaggga tgcaaatgaa taagtttatc gctgctgaag
    19921 ctgcggaatg tataggctgt catgcttgtg aaattgcctg tgcggtggca cacaatcaag
    19981 aaaactggcc gctgagtcac agtgacttta gaccgcgtat ccacgttgta gggaaaggcc
    20041 aggctgcgaa tccggtggcc tgccatcact gcaacaatgc cccttgcgtt acggcttgtc
    20101 cggttaatgc tctgactttc cagtccgata gcgtacaact ggacgagcaa aaatgtattg
    20161 gttgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc gatacgattg
    20221 cgcagaaatg cgacctttgt aaccagcgca gttctggcac gcaagcctgt attgaagtct
    20281 gcccaacaca ggcgttacga ctgatggacg ataaagggtt acagtggata aaggtggctc
    20341 gccagcgcaa aacggcagca ggaaaagcgt catcagacgc tcagccatct cgcagtgcag
    20401 cgttgctccc cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt gaacggaaaa
    20461 cccactttgg cgaaatctat tgcgggctgg atccactgca agcgacttat gagagtgacc
    20521 gctgtgttta ttgtgccgaa aaagctaact gcaactggca ttgcccgctg cataacgcta
    20581 ttccggatta catccgtctg gtacaggaag gaaagattat tgaagcggca gaactttgcc
    20641 accagaccag ttccttacca gaaatctgcg gcagggtatg cccacaggac cgtctttgtg
    20701 aaggcgcatg tactttgaaa gatcactctg gcgcagtctc tatcggtaat ctggaacgct
    20761 acatcaccga taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa gttgttcccc
    20821 gtagcgaaaa agtggcggtg attggcgctg ggccagcagg gttagggtgt gctgatattc
    20881 tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca tccagaaatt ggcggtatgc
    20941 tgacttttgg cattcctcct ttcaaactcg ataaaacggt attaagccag cggcgagaga
    21001 tattcaccgc aatgggaatc gactttcatc ttaactgtga aattggccgc gatattacct
    21061 ttagcgattt aacttctgaa tatgatgcag ttttcatcgg cgtggggact tacgggatga
    21121 tgcgagcaga tctgccgcat gaagatgcac ccggtgtcat tcaggctcta ccgttcctga
    21181 ctgcccatac ccgccagctc atgggattgc cggagtctga agagtatccg ctgacggacg
    21241 tggaaggtaa gcgagtcgtg gtattgggcg gtggcgatac gacaatggat tgtttgcgga
    21301 cttccattcg cctcaatgct gccagcgtga cctgcgcgta tcgtcgtgat gaagtcagta
    21361 tgccgggctc gcgcaaagag gtggttaatg cgcgcgagga aggtgtcgag tttcagttca
    21421 atgttcaacc gcaatatatc gcttgtgacg aagatgggcg tttaactgcg gtgggcctga
    21481 ttcgtaccgc catgggtgag ccggggccgg atggtcgccg tcgtcctcgt ccggtagcgg
    21541 gttcagagtt tgaattgccc gccgatgttc tgattatggc ctttggtttc caggcgcatg
    21601 ccatgccgtg gttgcagggc agcggaatta aactcgataa atggggcctg attcaaaccg
    21661 gtgacgtcgg gtatttacct acccagacgc atctgaaaaa agtctttgct ggtggtgatg
    21721 cagttcatgg cgcggatctg gttgtcactg caatggccgc aggaaggcag gcggcgcgcg
    21781 atatgttaac tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta tcgttaatcc
    21841 ggctgactgt attggctgcc gtacttgtga agtagcctgt gtggtcgctc acccttcaga
    21901 acaggagttg aatgccgatg tctttttacc ccggctgaag gtacagcgac tggacagcat
    21961 cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg cttgccccgt
    22021 gggggcgctg acgatgggcg agcaggtggt gcagaccaat tctgcccgtt gtattggctg
    22081 tcagagttgc gtcagtgcgt gcccgtttgg aatgatcacc attcagtctt tgccggggga
    22141 tacccggcaa caaatcgtga aatgcgatct ctgtgaacag cgggaagagg ggccagcctg
    22201 cgttgaatct tgcccaacac aggcgctgca gttgcttact gaaagagaac tcaggcgagt
    22261 ccgccagcag cgtattgttg ccagcggtga gaatcctctg tgaaagaaat ctccgcgctg
    22321 aaaggcgcgg agaatctatt agattgccca accacccgcg taaaatgcga ccagtgcggc
    22381 agtaatgatc accgtaccaa tattcagctt ttgccattcg cgtgcaaaga cgcggcctac
    22441 gaccagtgtc acaaagccca gcataatacc ggtaacgata ttacaagtca gaacgatgaa
    22501 cacggcacac accaggccag ccatagcgtc aataaaatca ttgaaatcca gcttcgagac
    22561 gttacttaac atcagcaaac ccacgtacat cagagcgggt gcagtggcgt aaccagggat
    22621 caaaaatgac agcggtgata agaacagaat cagcaggaat aacgccccca ctacggttgc
    22681 ggtcaaccct gttttacccc cggcggcggt tcctgccgct gattcgatat aaaccgctgc
    22741 gggcgctgcg cccaccagac cggagaatat tgaacttact gagtcactgg tcagggcttt
    22801 gccgccgttg atgatctggt tgtctttatc caacaaattc gcctgaccgg cgacggcacg
    22861 gatggtgcca gtggcgtcga acactgcggt catcaccaat gccagtacac tcggaagtac
    22921 ggttggctgg agtgcgccca taatatcgag gctgaaaatc agagacttac catcttcgcc
    22981 agtcagactt ggcatcgcca ccagaccgtg gtatttcacc gctgggtcaa agattaagcc
    23041 gataatcgaa attgcgataa tcaccaacaa gatcccgccg ggtacgcggc acttctccag
    23101 accgaagatg accgccagcc ccagcaagct catcatcacc gggaaggagg taaacgcacc
    23161 gagcgccact ggcaagcctt caatcgggtt tttgataacc atacccacac cgttagcagc
    23221 aatcagcagc agaaacaggc cgataccgat acctgtaccg tgagcgatac ccatcggtaa
    23281 attacgtaag atccaggtac gcacaccggt tacggaaata gcggtgaaga tgacgcccat
    23341 cagaaagacc gcgcccagtg cgacaggaac gctaatttgt tgcccgagta ccagactgaa
    23401 tgcggtaaat gccgtcaagg aaatcgcaca accaatcgcc attggcaaat tggcccacaa
    23461 ccccatcagc aacgagccga agcccgcgac cagacaggtg gcaacaaaca cagctgcggg
    23521 aggaaaacct gctttgccca gcattcccgg aacgacgata acggaataaa ccatggccag
    23581 aaaggtcgtt aagccagcca gtacttcctg acgaacggta ctgccacgag cggtaatttt
    23641 aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta ggatgtctcc
    23701 agacataatg atgtcctcac gaggatgtta aagttttatt attagttgcg ttcgtacacc
    23761 agacgaccat caacgtaggt gcggtagatc gaacggtcat cgcccaacgt catcatcacg
    23821 aacaatttgt cgactaaaga aacagagttg tcatagcgca gctgctgtag cggagtggcg
    23881 gtgggttcca tcaccacgaa atcagcctct ttgccaggta aaaagttgcc aatcaaatcg
    23941 tcaaggccca gagatttcgc tccgccgagc gtggccaggt aaaacgcttc atatgccgag
    24001 aggcgatagc cttgtaattg caatactttg taggcttcgt tcagcgtttg cagcatgttg
    24061 aaagtggttc cggcaccgat atccgttccc atgcccactt taactttctt ctgccatgct
    24121 tttttcaagt tgaataagcc gctgccgagg taaaggttgg aggtcggaca gaaagcaatg
    24181 ctggatttgg tttcgctgag acgatcccac tctttttctt cgagatggac gcagtgagca
    24241 aagacacagt ttttaccggt caggccgtac tgatggtaaa catccagata accatcatgg
    24301 tcaggataaa gcgatttcac ccaggcaatt tcatctttgt tttcacagag atgggtatgt
    24361 acccacgtat ccggatattc ttctttcagg cgttgcgcca tcgccatctg ttcaggagat
    24421 gaggtcgggg cgaagcgtgg cgtaatcgca tatagcagac gaccattttt gtgccagcgt
    24481 tcaatcagtt ctttgctttg gtgatagctg ctttcggcag tgtcgagcag ataatccggt
    24541 gcgttgcggt ccatcatcac cttaccggca atcatacgca tattgatatg actggcggct
    24601 tcaaacagcg catcaacaga ttgcggatga acagtgccaa acaccagcgc cgtggtggtt
    24661 ccgttacgta aaagctgctt gatgaagaat gccgacattt cgcgggcgta ctctaaatcc
    24721 tcataacgac gttcagtagg gaaggtgtgt ttattcaacc actccagcaa ttgttcacca
    24781 taggccccca ccatttcact ttgcggataa tggatatgtg tatcgacaaa gcccggtact
    24841 atcagtttgc cgcgatagtc gcgcacgcga atagtgtcag gaatttgatg ctttccgtct
    24901 tcccattcgc caaaccattc cacttttccc tgtttaatga gtaataaacc atcctcaata
    24961 aaccgcagcg cagaggcaat ctcttccggg ttatcgaccg tacgggtgac atcaataaaa
    25021 ctgcctcgta ccgctttcaa cgtgtgttct cctgacatca ttgactcctt tactgttaat
    25081 ccatctcttc cgctgaggta ataccaggca gaacgttttc ctgtcggtag ccaccaggga
    25141 ggataatatt taataaaatc gcagttaacc cgccagcaca aatagggttt tcaactagta
    25201 cataaataga ggctggcaat attttaaaaa tttcgggatc gtaggagacg ccaagtccta
    25261 aacctaaaga agtggcgaca ataagtgttt ctcggcgctt taaaccgttg gtgatgatta
    25321 tgcgaatccc agcgatggca atcatggaaa acatcaacgt cattgcgcct cccagaactg
    25381 ccgagggaat ggtcgtgaag aagccgccaa tcatcggaaa caaaccgagg ataaccagca
    25441 ttaccgcgat ggttcgcccg acataacgtg aagcgacgcc agtcatctga ataaccccat
    25501 tattttgcgc aaacgtggtt aagggtaatg acccgacagc ggaggcgata acagaaacca
    25561 gaccgtctgc cagcacgccg cctttcagtc gggactgata ctcttctccc tgaatgggtc
    25621 ggcgggaaac cattgccgtg gcggtgatat cgccgactgc ttccagcacg ctaagcagat
    25681 aaatcgtgcc aaccaccagg aactgatgga agctaaaact aaagccgtat ttgaacggat
    25741 gcgggatggt gattaacggc aaattgcgca tactgctgaa atccaccatg cccaggcata
    25801 acgatgcgat atagccgaca catagcccaa tggcgatccc tcccatgcgt agcaacggac
    25861 tgcgacagca gttaaagccg atcaccacaa ttaaaaccaa taaaccaacg ccgagatgtt
    25921 cgtaattgcc gaacgtaccg ctgcttttgg ctgcaaatcc tccaccaaaa tcgataatgc
    25981 cgactttaat caggcttaag ccgatcatca gtaccacaat accgctgacg gtaggcgtaa
    26041 taacccgacg taaatagggc aagataaatg aagatccgac aaccagaaat gcgccaacga
    26101 aggagacgcc gagaagcgac gacatgatta actcttcgtg aaaaccgtcg cttttcatgc
    26161 tgctgcccag cgcaatcatg accgtaacaa atgaaaaatt gactgactga attgagagta
    26221 ggccagaacc gacgatgccg tagcggttta cttgtaacca ggtaccaata ccagaggcga
    26281 tcatcgccat agaaacaaga taggcagttg tttcagcgga aagctgtaag gccgccccca
    26341 cgattaacgc gggggttacc atcggaacga aaattgccaa cagatgggta atggcaccaa
    26401 cgagagcctg atgaaaggga gggcgatcct ccagttcaaa tataaggtca gaacctgcat
    26461 tgtttatatc agacatccct tcactccttt taaagtaaat ttgatcatct gaattagtaa
    26521 aataaattcc cacgaatttg gggcgcagga cgccttgaga acggcttatc tcattttcgc
    26581 agtcactatt tagtcatcgt ccaaacagag aaacaggccg ccgaagcggc catttattat
    26641 ttatattttt tccagcgcag tgagtatttt ctccggtgtg aaatgccatt cgcgtaacca
    26701 gatgccgcat gcatcgtgaa ttgcggtagc aatcgccgga gctgcgccat ttacaccgat
    26761 ttccgagatc gatttcgccc cgaacgggcc gactttatcg tcgctcggca ccagcacagc
    26821 gcggaaatca cgcggaatgt caccaatttt cggtgcgccg taactgcgta aatcacgcgt
    26881 taacgggtga ccttcggcgt cataaatgat ctcttcgctc atactgtggc cgatagctcg
    26941 cagggtagca ccgtagattt gtcccaatgc taattccggg ttgaccggtg taccgcagtc
    27001 cagcaaggcg tagaatttat ccaggcggat ttcacccgta cgcgtgttga cggcaacttc
    27061 agcgaagttt gcgccatacg ggaaggcgaa atcaggcgtg atataactgc cagttcccac
    27121 cagtgaacca aagccggtgc cggtttcgcc tttatgagca atatccccga agctgacttc
    27181 gcctttcttg ccgcgcacga cgcccggcgt tgccagttga acatctgcca ctgcctcacc
    27241 caacatttgc gcgccgtgga acagaatttt ctcccgcaga ttttccgctg ccaaacgcgc
    27301 cgcgttacct gagaagcaag taccggacga ggcatatgcg cctttatcaa acaacgcatg
    27361 atcggtatca ccggagataa catgcacgtc ctgcggtggg cagtgcagca cttctgctgc
    27421 cagtttcgtc actacagtat ccagaccagt accaatatcc gcaccgccag aatgaacgat
    27481 aaaggtgccg tccgattcca gtttgatcat gcagttagcc tgatcgatat ccgggatacc
    27541 cgatttctgc atgataatgg cgacgccgcg accgatgtgc cagtcaccat tttgcggttt
    27601 cggtgacgac cattggatca tctcgcgacc ctgacgcagg atctcttcca gtgcacagct
    27661 ggcggcggaa ggaacggagg tcggcgcttt accttcaccg attgcaccga gaattttcag
    27721 ctcttgccct tcgtgtaccc ggttacgttc gataatttcc agttggtcga tctgtaactg
    27781 ttcagccagt tccgctaatg ccatggtgat agcgaagtta cctttcggtg cgccataacc
    27841 ctgataagca ccatttgggc aaatgttgct gtagtaggtg gtgacctgga aatcgacgtt
    27901 atcgcacgga tataacggca gcgacagcgc cggtccgtta cacggtacgg tgagtgagtg
    27961 gttgccgtaa gggccagtgt tggcgcggaa atccatcttc actgccgtca ggcgaccatc
    28021 ttttttcgct cccagtttga cggtgacttt cgcgacgtga cgagaggtgt tagcaataaa
    28081 ctcttcttca cgggtgtagc ggaacagtac cggacgcccg gtcacgcagg ttgcccaggc
    28141 gcacacttct tccagcagga tgtcctgttt ggaaccaaaa ccgccgccaa ctcgctcttt
    28201 aatgacatga actttatgct gtttcatgcc cacgaggcgc gcgacctggc ggcgtaagtg
    28261 ccatggtacc tgggtggagg cgtggataac cagacgatcg ccgtccatcc gggtaaagca
    28321 gatatgtgtt tcagtcgggc actgctgtgc ctgcgttgag ttataggttc gctcaatgat
    28381 cacatcggca tcggcaaagc ctttgtccat atcgccaata tgaccatgaa tactggcggc
    28441 gatatttttg cgagggcgag aaccgatcgg gaagttgatg atcatatgct cgccgcgctg
    28501 ggctgcatgg ctgttatcgt cttccagagt atctggcaca ccagcaacat acaccaccgg
    28561 ttcatcgtgc acgacaggcg catcttccgc cattgcttcg tcgatcgaca ttaccggctt
    28621 aagcacttca tattcaacgt cgatgagctt cagtgcttcg agcgcaattt cttcactttc
    28681 tgcgacgacc gcagcaacac gatcgccgac gtgacgcatt ttcttgccga acatacggcg
    28741 gtcaagcggt gacggttccg gtgcgctctg accacccggg gtatagtaga tatccgggca
    28801 attcaggtga gtaataacgt gaacgacgcc cggtaaggct tcagctttgc tgacatccag
    28861 atgagtaatc agtgcgtgag cgtgtgggct acgtaacatt ttaatgacgc aggcgtcagc
    28921 cgttacgcgg tcttcaacat agcagggttt cgcctgcacc attttcgcgg catcagtttt
    28981 aggataatgc ttaccaatga cttctaggtc gtcacggaaa gtcggagcga tatcaatggt
    29041 ggcctgcgga ttatttttac gtgcaaccgc cagttcaatg acctgatagt attgctgcca
    29101 gccagcatcg cggctgaaca aaccagaaag cgcgtcgtcg atctcttcgc gggtaggtgc
    29161 ggcgatgcga tcgagaagat cggtgataat cagagctgca gctggatcgt tataaccaga
    29221 ctgcaccacg ccaacatcaa ccattgcctg ttgaaccaga cttaactcgt tccatttgcc
    29281 cagagattct gcggtacgaa tatctgcctt ctctaactgt gcggcaataa gcaaggacgc
    29341 gttaacgata ttaccgttaa agattattgc gtcagaacca gcaaacccga agccatcatc
    29401 actgttgcgt acagagtgca ttcccatgtt aaacaacagc ttttgcacgt tttcgcctgg
    29461 attaacggtt agctcctgag gcgcgccatt taaagtaaag tggatgatca tacggcttcc
    29521 tcctcagcct gttggcagtc ggcatacagg tcggctacca gaactcccga gatatagcgt
    29581 ttataggcca cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc gacagcctgt
    29641 tcaagtgcat tgccttccag attttgtttt tcgacatcat gcagacgcag tgctttactg
    29701 gcgacgccat ccagcgcaat tcgcataccg tcgtggtctg tcattgcaac ggctgccgtc
    29761 acgacggtta aacctgcctg agaacggcta attttgcggg tcgcacaggt gcgatacgga
    29821 tctttaatga taatttcggt tagcaggcga tcgcatgggc aggccaggta gtcctcgatt
    29881 gacagcgttt cgccgttgcc aaaaaccagt tcagcatcca gtgccaacag gacgggaagc
    29941 agcaccgact cttcctggcg ggcggcgatt tcaccaccaa tggtcgactg attacgaaca
    30001 tggcgtgagt aaacaaaacc gagggcttca cgcagcgctg caggaataaa tcgcgcatca
    30061 cgcagtggct gcaagcgaga cattgcgcca atccgcagtg caccgttatc ccagtcaacc
    30121 cagtccagtt ccagatcctg taaggaaatg gcaatctttt tatcggtacg ggttggtgta
    30181 gcattgagtt tgctgccccc ggcgaaccag acggcttcat cctggtagcg gcgcttcagt
    30241 tccagcgcct gttcgacaga gtcgggcctg aaaaattgtt caatcatctt ctttccttat
    30301 ttaaccctct tccctgcggg aagagggcat ctgtcattta tgccagcgca tccatccgac
    30361 gccacatact ggcagcggct tttctggctt gtgcataaat ggaatcgcaa tcgaagttaa
    30421 actgacggtc ttcatagacc atcacaccat tgaccattac gctgtgaacg ctgcctgagc
    30481 ccataccgaa agcgatatgc ccggcgatat tgtctgccag cagcggcgtc ggcgagttgt
    30541 aatcgcaaat ggttaaatca gctttgtaac cggcttccag aagcccaaat ttcgcgccaa
    30601 agttgcggct catcagttcg ttgccattag tcagggcttt ggcaaaactg tcaggccaca
    30661 gcggaccacc cgcatcgcga tgtttaaaga aggcaaattt catctcttca aacatgtccg
    30721 aaccaatgcc gtccgttccc aacgccagat tgcggatgtc gctaaggtga tggttgtagc
    30781 cgacatggtt gttcatgttt gaacgggcgt tatgcaccag gaacgcatcg cgctgattga
    30841 gtagggtgat gtcatctttc gacaagtaaa gcccatgagc gaccagcgtt ttgctgtcga
    30901 tgagatcgaa ttgcgctagt cgtgccagca ggtctttgcc gtaccagtgg tgactgtagg
    30961 aaacgtcgta aaggtcttcc gcagcgtgaa tatgcaaacc acggcctgtg gctttcacgg
    31021 cttcacgtag catctccaga ccggcatctg gcacggtaaa cggcgcgtga gcaccgatat
    31081 gtgcttccac cagatacggc tcggttgctg ctttcttcgc ctcatcaatc tgacgggcga
    31141 agcggatgtt ttcttctaca ccttcctgca actctttgat gccgttgtta cggtcagtag
    31201 tttcaaaaca ggtcatcgcg cgcaggccaa cttttaaaaa tgcgtcgcgc aatgtggaga
    31261 gcgacccgcc gatatacgcc ggagaggcat ggtgatcgat aaccgatgta catccgctct
    31321 taatcgcttc cagggaacaa atcagcccgc tgtaatagag cgactcttca tcaagggcgc
    31381 gatcgagccg ccaccagaga tttttcagcg ttgagatgaa atccgggcag ggggcgatgt
    31441 ttgccataat tccgcgggaa agccccgagt aaaaatggtt gtgcgagcag acaattcccg
    31501 gcatcacaat ccgaccatgc atctctttgt agctggcatc ggggtagcgt tgcgtcaggg
    31561 catcgccgat agcgacaatc acatcatttt cgatggcgat atcaacgcct tcctgcactt
    31621 tcgccgggtg taactgtact gcggtgacat tcttcagaat caacatgatt acacctccac
    31681 gcggcccagc agataatgat gatgctggtg gacatggctg atgatgcggc acatatcgtt
    31741 cagctccggt ggtacgttgt cgaactggcc ttcgctgtcg atgttcagca ccaagctttg
    31801 gttattcaga cgtacgcgta cccgacaatc ttccaccagg aagcctgggt tgctgctgtt
    31861 atcaaagtct tgcgacaggc tgaagacggt gattttgtct ttgtacggtt taccgttcca
    31921 cggacagaac tgagcgcagt tgccgcattc gttacagtaa gcgtcgaggt gcagcgtctg
    31981 gaaacggttc tggaagcctg ggaccgcaat ggagacgttg gcgcggttcg ggcagacatc
    32041 cacacacttg ctgcaaacgt agttacattc gaggcagcga gcggcttcct gcgcgacaaa
    32101 cgcatcacga tcgtcactgt tcaccagcgt gatagagata tcgcctttac gttgatagat
    32161 ttccgccgga ttgacgttgt tccagtattt attgttctgg tgggaacgga tattttcccg
    32221 actaaggatg gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa tggaggatgg
    32281 tccgcgctgt acgtcgccga tcataaagac gtcagtcaga cgagtttcgc cattatggtc
    32341 gacgtctggc cagccgtttt tgtccagcgg tacgcccatc gcattcaggg cttcagtatc
    32401 ctgctgttca ccgatggcgg tgatcagact gtctacatgc agtgttactg tttcgttggt
    32461 ttcaaccgga cggcgacgac ctttctcatc gggttcgcca agcgacataa cgcgcaaggt
    32521 taaggtgcca tcagcatcga aacattccgg attattcagg aaacggaact ccacgccgtc
    32581 gtgcaacgct tcttcatact cttcgcgcca tgcgggcatc tcttgcagtg aacgacggta
    32641 aacgatcgtt gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc agtccattgc
    32701 ggtgttaccc gccccgacaa cgaccacatg tttgcccagc ttgagcgctg tacccttgtt
    32761 gtattcacgc aggaagggga gtgatttcca gacattttgg ttgtcgcccg ccagtttcac
    32821 accgctattt ttatcagtgc cggtggcaat cagaacatag tggaagccct gattttttaa
    32881 ctgttcaacg gtcaaatcgg gtgagcagcc atactcaaat ttcacgccgt gagcggcaac
    32941 aaaatcgata tcgtgctgaa ttaactctgc aggaatacgg aactgaggaa tgatattttt
    33001 caccacgccg ccagcattgg cttcgcgttc aaacagcgta accggatggc ccgctctggc
    33061 aaggaagtaa cctgctgcca gacccgccgg acctgcacca atcacggcaa ctggatggcg
    33121 tgaaccagaa ccggctggtt tgtgccagcg ttgcttatat tcatcccaac ctttttccag
    33181 cgcgactttt ttcagttcgc ggatattcag cgcactgtcg taatccaggc gggtacagtt
    33241 gtattggcac tggtgatcgc aaatatgacc ggtaatggcg ggcagagcgt tgcgttggta
    33301 gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc agacggatgt attccggaat
    33361 atcttgcttg atagcacagg cagtaacaca gggggcaacg tagcaatcgg tcagcggcag
    33421 gtcttctgcc acttcaatac gctcttctgg cttccagtgt ttctgggtgt attccatggt
    33481 taacgcatct gctgccagtc tgttcagtcg ttcgacgtca acatggtcaa gcccccaggc
    33541 gtcggagcct tccagctcgc gcatgcaggc acttaagcgc agatagccgc ctggtttcag
    33601 caggtcggtt gccatagtaa tagggcgaat accagtatca aaaatatcgc ggatagtcag
    33661 ctgactggca ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc gagagagaac
    33721 tgctgcaaca ttgatggaga gcgggaacag cgcacggcct gacatataca tctcttcacc
    33781 aggcagtgcg cctttattgt tgatggtgcc gagagtgtta gtcagtttta cgccaaagcc
    33841 gagtgatttt tcttttgcca gtgccatcag gcgttccagc atttccagtg cttgcgtcag
    33901 cttgaggtcg tgatcaaatg actcttcttt taagcctatg taaccgaaac cgcagacatc
    33961 gaggatctca cgaacacgcg cgtatcccag taaggtcggg ttaagtttca caaaggtgtt
    34021 gagccctttt tcttccagca tgtagcggca aatggcttcg atttcatgcg gaggacagcc
    34081 gtgcatggtg gagagggtga cgccatgcac catactggtg gggatgcgag cgggtaaggc
    34141 ttgcaagctt tcgcgttttt cctgcaatcc gtggcgggct aaaaatgcgt catcctggag
    34201 taatttattc agcgtatcgc gatattgagc gaatttcgga tggtcagatg cgtccatcat
    34261 attgtcgatg aactgttgca tcggcggttg cttaatacct tcgaggttgt aaccgacgct
    34321 catattaaag atgaacgatt taccggaatc agaaggctgg aacatcgctt cgagaaggtg
    34381 cagggcaaac caggctttga ggtattcatc ccaggcttta agcagggtaa attcggtaga
    34441 ccattcggtg ttaaaacact cgtcttcggc atcgatacag ggcttttcca gctccaggcg
    34501 gtcaagaatt tggacggttt ttagttcgat gaagcgtccg ccagtcagcc aggaagtgac
    34561 aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg ccgacgggag tggcacaggt
    34621 ttcaccgaag acgctaacag ttttaccttt tacgggtgag taaaactgtt gctcgggaat
    34681 accaaagatt gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa gttcctcaaa
    34741 cggaatggga cgcataatat cccccataac cctctcctta gcttatacag atatcattga
    34801 gtgaccggtg ttgagaaatc cggtggttta gggggataga gcaacaatcg caccactctt
    34861 gccaggtttg gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa gggctttttg
    34921 tgaatgaact cacaggtgcc aaatgagaat taaaatcact cctgaaaatt attattgcca
    34981 gcccatttat caatttttct cttttctcaa aatcagtgat tccttatcat tatgataatg
    35041 agtgccggaa ggattaaata tttgaacgca atcgtttaac tacgttttct aaatttcagt
    35101 atacctttcc ttcgctgtaa taaagtcgtc cggagtatca atatctaaaa ttatttctgc
    35161 attttctatt tcgacggaat aatgatctcc cataaggagt gcctgacgca tattggtgac
    35221 attgggtcgt tggattgcct gtatcaagca tggttttgac actaaaatcg gatggccggg
    35281 gatgccatta tggagtggca gtattgcgcc atcatttcgt aacgaccaga tttttctaaa
    35341 aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga aaacaatgtt ctgtttgcac
    35401 tgcgggtact gcggccttga cggatgttag taaaccctgc gcataatctg ggttgtgaat
    35461 aatagtgata ttgctctggt tcgcataacg ttcgtgcagt tcattaccac gatagccggt
    35521 gactaaaata attcggctac aaaactgcaa cgcatttttg atacttgtat caagaattgt
    35581 tccctgttcc cagggtaaca tcattttcca ttgccccatt cttgatgata atccggcagc
    35641 ggtaattata cagtcgatgg ctgacatagt ggttccatta tttatagggg ctaataacgc
    35701 ctgataattg cgttacttcc tgatggatta agttggggat aatttatccc agagaggtca
    35761 taaagactcg tgaaaagtat agttgaccca tcggcattag tcattgattt aggtgcgcag
    35821 aaacgtccca ctgtgatttc tgttgttggt gctgggggga aaaccagctt gcttttttgg
    35881 ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa tcacgacgac gacacatatg
    35941 tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg atcccgccat gcttcctcat
    36001 gcgtctctta catcccccat ttcattttgt tttcacagct ggaaagcgaa ccagggaaaa
    36061 gtgcagggat ttacgccaga agcgattgat gcactggtgc aacgaccaga gtgtgacgta
    36121 attctcattg aagcagatgg ctcgcgtgga atgccgttaa aagcgcctga tgagcacgaa
    36181 ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga tgggaggaca tactttgggc
    36241 gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt ttgctgatat taccgggtta
    36301 acacctgatg caaccttgca actgagcgat ctcgttgcgc tggttcgcca ccctcagggg
    36361 gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt tcattaaccg tttttctcaa
    36421 tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc tgcaacaaca cgacgtagag
    36481 gcaatctggc tgggcgatat acaagaacat cctgcaatcg cgcgcagatt tgtgaattag
    36541 cggcccgggt attccgggtt actgaaagga tatccattga ggttggtatg aatattttca
    36601 cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc gatggcgcaa attgttgata
    36661 gccgaggctc gactccccgc cattctgcac aaatgttagt gcgcgccgat ggttctatcg
    36721 tcggtacaat tggtggcgga atggttgagc ggaaggtgat tgaagagtca cttcaggcat
    36781 tgcaggaacg taagccgcga ttattccatg gacgtatggc tcgtaacggt gcggatgctg
    36841 tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag cgtccatggt atgcgtccac
    36901 gtctggtgtt gattggcgcg gggcatgtca accgggcgat agcccagagt gcggcgctat
    36961 taggatttga tattgccgtt gccgatattt atcgcgaaag cctcaatcct gaactattcc
    37021 ctccatcaac cacgcttctc catgctgagt cgtttggtgc agcagtggaa gcactggata
    37081 ttcgccctga taattttgtc ctgattgcca cgaataatca ggatcgtgaa gccctcgaca
    37141 aactcattga acagcccatt gcatggttgg ggttgctggc aagtcgccgc aaggttcagc
    37201 ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga acatattgcc cgtttacatg
    37261 cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga gatcgccatc agcgtgctgg
    37321 cagaaatatt acaggtgaaa aataacgcgc cgggtgggct gatgatgaaa ccttctcatc
    37381 cttccggaca ccagctggtg gtgattcgcg gtgcgggaga tatcgccagt ggtgtggcgc
    37441 tacgtctgta tcatgcgggt tttaaagtga tcatgctgga agtggaaaaa ccgacagtga
    37501 ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg cgaaatgacg gtcgaaggcg
    37561 ttactgctcg cctggcaacc agctctgcgg aagcgatgaa acttaccgaa cgcggattca
    37621 tccctgtgat ggcagatccc gcctgttcat tgcttgatga actgaaaccg ctttgcgtgg
    37681 tggacgctat tctggcgaaa cagaatttgg gaacgcgggc agatatggca ccagtaacaa
    37741 tcgcgcttgg gccgggcttt actgcaggga aggattgtca tgcggtaatt gaaacaaatc
    37801 gcgggcactg gctcggtcag gtgatttact ctggttgtgc gcaggagaat accggtgttc
    37861 ctggcaatat tatggggcat accacccgac gggtgatccg tgctcctgct gcaggcatta
    37921 tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg cgatgtgatt gcctggattg
    37981 gtgagcatga aattaaagca ccgttgacgg ggatggtgcg tggcttgttg aacgacggcc
    38041 tggcagtggt cggtggtttt aaaattggtg atatcgatcc tcgtggtgaa acggctgatt
    38101 tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg cgtacttgag gcgttaatga
    38161 tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct ggaagtggct taaatgaaaa
    38221 tggccggatg gtattaccca cccggcctta aaattactga caaatcaccg ttccggtttt
    38281 gccttcaatc ccctctttcg ctttgctcaa tacggtaatc agtgcttcac gacctgcgcg
    38341 agagcgagca aacgaggcgg cagcttccac tttaggcagc atagaacctt tcgcaaaatg
    38401 accttcttcg attaagcgtt cagcatcact taatgacagg cgatcgagcc actgttcatt
    38461 ctctttaccg aagttaatgg ccactttttc taccgcagtc agaatgatca gcatatcggc
    38521 atcaatcatt tctgctaaac gggcgctggc ccagtcttta tcgataaccg cgctggcacc
    38581 acgcagatgg ttaccttcac gaataacagg aataccacca ccgccaacgg tgatcaccac
    38641 ctggcctgca tctaccagag ctttaaccgt ttctttttca ataatatcaa ccggttttgg
    38701 cgaggcaaca acacggcgat agccgcgacc agcatcttct ttcagagtgt aaccctgttt
    38761 tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg atcggcttgg tcgggttgag
    38821 gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc agcgttgcta ccggtttatt
    38881 aatgccccga gaaagcagtt cttctcttaa ggcgttttgt agatcgtaac caatataacc
    38941 ctggcttaag gcaacgcaga cagacatcgg cagcattggc gagtgcgctt cagtttttgc
    39001 agcagcttca aaggcctggt taatcatgcc cacctgcggc ccgttaccat gagtgacgat
    39061 aacttcatgt ccttgggcaa ttaaatcaac aattgcctga gaagtaattt ttaccgcttt
    39121 catttgtccg gccaggtcat cgcccagcgc attcccgccc agggcgagaa caattttctt
    39181 actcattttt cctcactcat cattcttgat tgtgtataga tccctgacag tggttagagc
    39241 acgggaggga caaacggttt gcgtcgcagg aaacgacctc gcccggcttt gcctgtaaag
    39301 gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc tgacaatcgc gccctgacag
    39361 gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga gatggcgatg ctgaatttgt
    39421 tggctctgac gtgggtcgat aatcaccacg tcgccatcgg aaccgggcgc taataatcct
    39481 ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc tggttaattc aacaaagcgt
    39541 tccggtgtta tacgtcccgt catcacgcca ctggaaaaca gtaactgcat gcggttttcc
    39601 acaccgggta agccatttgg gcagcgactg aaatcgcctt tagaaatttg caggcgttga
    39661 gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa tcgcaccatc gctgatgcca
    39721 caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg ggctaagaat gaacttcatg
    39781 ccgtcttctg tatcgtaact gcgttcgtcc aacaggagat attgtgggca ggtttcaacc
    39841 cagactggct ggtgattcgc acgggcaaga cgcagataat ccagacctaa gccgttagac
    39901 aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg ccaggttaat catgcgggcg
    39961 atggcttccg cttcgcattc cagagggcga ctcaaggcat gatagcgcgg cgcggttaac
    40021 cctgcggcga taaattccgc ccgcttgctg gcgatagccg catcattttc cgggtgcacg
    40081 gtggtcagcg cgccggattc atgcagacgg cgtaatgcct gcaaaacctc gtcatcgttg
    40141 agtttgtatt gataggttaa atagagttta aaactgctca gcccttcctc gaccatcata
    40201 gggatttcgt cgaggatggc gtgattaatg tgctggatca caccatgaaa gctgtagtcg
    40261 atgaccgcct tatgggcggc atagccacga taaacctcca gttgatggcg taaccgacac
    40321 ccgtttgggc caaatcccat atggtcaata atggttgttg taccgccaca cgcagctgcg
    40381 cgggtaccgg taaaaaaatc atcacaactg cgcgcgatgc cgacatcaat attgaaatgc
    40441 gtatggacat ccacgccgcc agggaaaacg taacagccag tggcatcaat ttcttcatac
    40501 gggagctgcg gcgaaatatt gttgcccaac tggcgaacaa tcccgctttc aatcagcaaa
    40561 tcctgtttgg cttgtccatc tgcgttaacg acagtgccgt ttttgatcaa tacgcgcata
    40621 gcaaactcca aaaaccctcc ggcgaaccgg aggggaataa gtgaagagta attattcggt
    40681 tgccagccag cttaacggga ttgcagcgta catcgcagca caggtcacca ggtgagattt
    40741 ccaggttttt tcgttaggtg catgcgcttc aggttcttta cccgggccaa agccgatcac
    40801 cggaatgccg tgacggccca tgatagatac gccgttagtt gagaaggtcc acttatcaac
    40861 aaccggcgct ttgccaaaca gcccttcgta ggcattcacc agtgctttaa cggtgaagtg
    40921 atcttcttcc actttccagg tcgggaagta gcattcggtt gggtaaacca ggccagtcca
    40981 ggacggacgg tcgtagttgt acatagaaac aaccgcgtta gctttctgta ctgcaggcag
    41041 ggcgcggatt tcgtccagcg cgccttccca ggtttcgccc caggtcagac ggcggtcaat
    41101 agagacggcg cagctatctg ctacagcgca acggcttggg gatgtgaaga agatttcaga
    41161 aacggtgagg gtgcctttgc cgaggaattc gtcataaccc agacgttggg agagttcttg
    41221 taattcgcca agaatcggac ccattttgaa aatggcgttg tcaccgcgtt ctggcgcaga
    41281 accgtggcag ctaacaccct gaacatcaat acgaatttcc atacgaccgc gctgaccacg
    41341 gtatatctgg cagtcggttg gttcggtact gaccacaaat tccgggcgaa tgccggattg
    41401 ttcaataatg tactgccagc acagaccgtc gcagtcttct tcctgcacag taccggtaac
    41461 cagcagggta tattcatctt ccagaccgag gtctttaatg attttaccgg cataaaccat
    41521 agatgccatg ccgccttcct ggtcggaagt accgcgacca ccgattagct catcagtttc
    41581 catgccttcg tacggatcga agtcccagtt tttgatgttg ccaatgccga cggtatcgat
    41641 atgagcgtcc attgccacca gacgcgggcc gtggccgata taaccgagaa cgttgcccat
    41701 cgggtcgatt tcaactttat cgaagccgac tttttccatc tcttctttaa tacgatgtac
    41761 tacgcgtttt tcgtcgcagc tttcactggg aatagcaacc atgtcgcgca ggaagcgagt
    41821 catgtccgcc tggtaatctt ttgctttttc aagaatcagt ttgaatggaa tattcttagc
    41881 cattattttt ctccagattt tttactcccc gttttgcagg gagtagttcc aatagggtga
    41941 ttaaggtgct acagcgtgtt tgccttccca gacaacttcg cggtagtgct tcacgtcggt
    42001 gtcgccttca gtgctgataa ccagcaccac ggcatctttg ttcagcgcca gtttttccat
    42061 caggctttga cgttgcgggt gataatgaac cgctgcgaga acgcccaaac cgacagcgcc
    42121 ggattcaccg gagatgatgc gcgggtcgtt gccgtacgga ttacccagca cgcgcatacc
    42181 taatgcggca acgctgtcct ggcaggagat gaattgggtg gcgcagttac gtaggatttc
    42241 ccagcccagc gggttaggtt cgccacaggc caggcccgcc atgatggtgg ccatatcacc
    42301 gccaacgttg acgatgtcgc ctttgacgcc ggagcgataa atacagtcag ctttgtcagg
    42361 ttcaacaata atgctgtgca gattttgcgg gctgtagacg tcgaccagat aacccagcac
    42421 accaccggcc attgctccga caccggcttg cagcagaacg tgcgtcgggg ttacgcccat
    42481 ttcacgcatt tgctcgacgg cttcatctgc cagggttgcg tagccttgca tgatccaggt
    42541 tgggattttg gtgtaacctt cccatgccgt gtcctgtacc acttcccagc cgtgctgctg
    42601 cgcgtgttgc atggtcaggc gaacggtatc gtcatagttc atatccgtga cgatgcactc
    42661 ggcaccgagg ttcagaatgg cgtcaacgcg ttcctgagca gaacctttcg gcatgtaaat
    42721 caccgcattc tgtccgagtt gctgtgctgc ccacgccaca ccgcgcccgt ggttgccgtc
    42781 ggtggtggtc gcgaaagtca ttttttcgcc gatggcattt ttcaggtgct caaatgacag
    42841 cgtttcgata tcaagatgat atttttcaca caataattga gcgatggcgt acgcaccgcc
    42901 aagcattttg aaggcgttca gaccgaatcg ttttgattcg tctttgacga gaattttctt
    42961 cacaccaaaa aggtttgcga gatcgtccag cgcacaaagc ggtgttgggc gataaccagc
    43021 tattttctgg tggaactggc gcgccagttt ggcctggctt tgcgaaaaga gcggtgatgt
    43081 ttcgccgttg aaaaatttgt tatcggcgat atcaatcttc aatgagaaaa cggacatatc
    43141 ctatcctttt ggcctctgga aatagagggc cgaccagctg atcggcccga ggggttattt
    43201 cacgcgttct tgcgcttctt tcagcagttg ctcgagcagt gcacctggtt tggcgtattt
    43261 acgggacagg atcatcgcgg cgatgatata aggcttccag ctggcttctt tgtaggttgc
    43321 gatacggtat ttttcgaata cgccttcagt cacttcacct tctttacagg atacgccgct
    43381 gatatcagct ggcaggcagt gcatgtacag ggcttcgcca tcacgggtca gttccatcat
    43441 ctcttcagta caatgccagt ctttgtgttg cgcgttctgt gccagacact gtttttccag
    43501 tgcttttaag ccttcgtgat cgttagcgcg cagcaattcg gtacgctgtt ccatcacttt
    43561 gtaaggtgcc catgacttcg gataaacgat gtctgcatct ttgaacgctt cttccatgct
    43621 ggtgacctga cggaagctac cgccagatgc tttagcattg tttttcgcca cttcaaccac
    43681 atccgggatc aggtcgtagc cttccggatg ggccagggtg acatccatac cgaagcgagt
    43741 catcagaccg atgatgcctt gtggtacaga gagcggtttg ccatagcttg gagagtaggc
    43801 ccaggtcatg gcgatttttt tacctttcag gttttccagt gaaccaaagt gttcacgtaa
    43861 ccacgcgagg tcagccattg actgagtcgg gtggtcaata tcgcattgca ggttcactaa
    43921 agccggacgc tgtggcagta caccctgctt gtaaccgtca tcaagtgcag cgccaacttc
    43981 acgcatatag gcgttgcctg cgcccagata catatcgtcg cgaataccaa tagcgtcggc
    44041 gcagaaggag atcatattgg cggtttcacg cacggtttcg ccgtgagcga tttgtgattt
    44101 gccttcgtcg agatcttgtt gtgcaaggcc gagcaggtta agcgcggaag cataagagaa
    44161 gcgggtacgg gtggagttgt cgcggaatac ggaaatacct aatccactat taaagacttt
    44221 ggttgagatg ttttcagcac gcagtgcttt taatgctgcg gcaacgtcca gtacttgttt
    44281 cagttcatct ggcgtctgtt cccacgttaa caaaaaatct ttctcgtgaa ggtgagaggt
    44341 cagcgaattg atatccttaa tcagctcatt aacagttttc atcataaagt cccggtagtg
    44401 gaatgacagg caaagtggtt tatttcactg tgacttgcca aagtgattgg tgttttcaca
    44461 gctatacctg ggactaacaa taagtgtgcc agaattatcc acggggctgc aaaagactaa
    44521 tttgacggat aatgtgtggg gttgatcaca aattgatgcc agcttccggt tgtttgtaaa
    44581 atacgaaaac gtttggcttt ttttatcgcg ttatttattt gctgctaagt atcagaaata
    44641 agataaaggg acaggctatc tctcttcagt ttgataaact caccgccgta attctcatat
    44701 tgatatagaa atgtgatatc tcttccataa tttcattaga aaatgataaa aatcttatgt
    44761 ttttatcaaa gccaatttcg cctgagttaa tatcatcatg atctgtgaac catcaacgtc
    44821 ttcaatgatc gacttttaag taagtctgga aggtaaagct atggagcttg ctactacgca
    44881 gtcagtattg atgcaaattc aaccgacaat tcagcgtttt gccagaatgc ttgccagcgt
    44941 tttgcagctt gaggttgaga tcgttgatga aaacttgtgt cgcgtcgccg gaacgggcgc
    45001 gtatgggaag tttcttggtc gccagttgag cggcaactca cgcctgctcc gccacgtcct
    45061 ggaaacgaaa actgaaaaag ttgtgacaca gtctcgcttc gatccccttt gcgaaggttg
    45121 cgatagtaaa gaaaattgcc gcgaaaaagc atttctgggt acgcctgtca ttttacagga
    45181 tcgttgtgtt ggggtgataa gtttgattgc cgttacccac gagcaacaag agcatatcag
    45241 tgataattta cgcgaatttt ctgattatgt tcgccatata tccaccattt ttgtttcgaa
    45301 acttctggag gatcaggggc caggagataa catcagtaaa atattcgcga ccatgatcga
    45361 taatatggat cagggcgtat tagttgttga tgatgaaagt cgggttcagt ttgttaatca
    45421 gactgcctta aaaacacttg gtgttgtaca aaataatatt attgggaaac ctatccgttt
    45481 cagaccatta acatttgaga gtaattttac tcatggacat atgcagcata ttgtttcgtg
    45541 ggacgataaa agtgaattaa tcattggtca attgcataac attcagggcc gacaattatt
    45601 tttaatggca tttcaccaat cgcataccag tttttctgta gcaaatgcac ctgatgaacc
    45661 acatattgaa caattggttg gcgagtgccg tgttatgcgg caattaaaac gactcattag
    45721 ccgtattgca cccagcccat ccagcgttat ggtggttggt gaaagcggca cgggtaaaga
    45781 agtcgtcgcc cgagcaatcc ataagttgag cggaagacgg aataaaccct ttattgctat
    45841 caactgtgcc gcgattccgg agcagcttct ggaaagcgaa ctgttcggtt atgttaaagg
    45901 cgcatttact ggcgcttctg ccaacggtaa aacagggttg attcaggcgg cgaatacggg
    45961 cacgctgttt ctcgatgaaa taggtgatat gccattaatg ttgcaggcta aattactgcg
    46021 cgctattgag gcgcgtgaaa ttctgccgat tggtgccagt agcccaatac aagtcgacat
    46081 tcgcatcatt tctgcaacta atcagaattt ggcccagttc attgccgaag gtaaattccg
    46141 cgaagatctc ttctaccgac ttaatgttat cccgataact ctgccaccgc tgcgtgaacg
    46201 tcaggaagat attgaactat tggtgcatta ctttttacat ctgcataccc gtcgtctggg
    46261 atcggtttat cctggcattg ctcccgatgt cgtcgaaata ttgcgtaagc atcgttggcc
    46321 cggaaacctg cgcgagttaa gcaatttgat ggaatatctg gttaacgtag ttccttcagg
    46381 tgaagttatc gacagcacgc tattgccgcc aaatctgctg aataatggca caacggagca
    46441 aagtgatgta acagaggtca gtgaggcgca cctgtcactc gatgatgcgg gcggcacggc
    46501 gctggaggag atggaaaagc aaatgatccg cgaggcgctt tcacgtcata acagcaagaa
    46561 gcaagttgct gatgaactgg gcatcggcat tgctacgctc tatcgcaaga ttaagaaata
    46621 tgagttgtta aacacataac ctcagtgaat ccggcctgaa ttcaggccgg atatcctttt
    46681 acttcgtttt ctcgcaatcc agaactgtat ttacaatcat ctgatacccc gtgcagcgac
    46741 aaagatttcc cgccagtccg cgacgaattt ccgtaatggt taatggcttc tcgcgtggtt
    46801 tcgccagcat tgccgtggta gccataatca ggccaggcgt acaaaacccg cactgcactg
    46861 cgccggattt cgcataagcc tgctgaacat gagaaagttt tccgcctttc gcttcacctt
    46921 ccagcgtgcg gatctctttt ccttcagccc aggcggcaag gtataagcaa ctgtctattg
    46981 ctgtgccgtc gaccaacacc gtacaggcac cacattcacc cacgcagcac ccttgtttga
    47041 cacttagcag tccttgttcg cggagtaatt ccgagagcgg cgtgcctggt gcggcgtgaa
    47101 gctgaaaagg catcccgtta atggtgcatt cgatggtaat tgtttcgctg tgattcattg
    47161 caattttccc cccgccgcgg cgacggcttc gctaatcact tttttggtca tcgtctggat
    47221 gagatgcaga cgaaactctt tactggcccg ccatgaagaa cgcggggcga catcttgcag
    47281 gacagattcg ctgatagctt ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg
    47341 ttcggcatgt tggcagcgaa tcggcgttgg cgcggcaaca ccaaatgcca ggcgtaattc
    47401 gctgaaattg ccgttatcca gtcggcaatg tgcggcgcag ccaatcgttg aaatatccat
    47461 tgcgtcgcgc atggcatatt taaaatgcgc gctgcccgcg tgttctttcg gctgtggcgg
    47521 aaaatgaaag gcgacgagga tttcgtcatg ctcaagagac actttgcccg gcccggtgtg
    47581 aaagccatta atcgggacga aacgaacacc gcgcggggag tggatctcca gtttcgcgtc
    47641 ataaattagc gttggcgtgg cagaatctgc gctggtggca ccgttgcaaa tatttccacc
    47701 gtaggtagcg acgttacgga tctgcggtcc agcaatggac gtggccgcag cacataacgc
    47761 cgggagatga cgttgagtta taggatcttc tattagctgg gtaaatgtcg ttgcagagcc
    47821 gatacgtagc gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat
    47881 atcaacaata tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc
    47941 accggcgagc agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt
    48001 tgctgcgcga tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca
    48061 gatggaactc ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg
    48121 tagccatctt caccgcgtta cgaatagcag cggcaacagg aattattggt ggctcaccca
    48181 gtgacttatg tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt
    48241 gtggcagatc cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc
    48301 cgcttttagc atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc
    48361 cgccgtgtac ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt
    48421 tgaggatgcg gttgatgatg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg
    48481 tacagccaaa cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc
    48541 cgccgcgttc agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct
    48601 cttccggtcg ttcaaccagc acgatatggc cttttatcag ggtcagattc atcgctgact
    48661 gatgtagcat gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc
    48721 gcagcgcagg cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg
    48781 taacgtcggt atcttgagtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt
    48841 ctgccaccat ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt
    48901 gcacgttgat ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga
    48961 caggccaggt gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc
    49021 cttgctggtt ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt
    49081 caagacactc cggcaaccct gcgctgtaaa tacgtttgcc cgtgagcgga ttagcatctc
    49141 cttcgcgggc ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg
    49201 cgtcatcaag catagactca acggcaaata cgacttgtgg cgcgccataa ccacgcatcg
    49261 caccagccga ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag
    49321 gataaaggta agcgacttta ttcccccccg cagaagcgat ggagtgcccg tgagatgcat
    49381 aagcgccggt gttagacaga acatccagac tataaccttt caatgttccg tcgcggttca
    49441 cgcccatttg cccgtcaatg gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt
    49501 cacggctaag ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca
    49561 ttggctcttc cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga
    49621 ctcgtacgca tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg
    49681 ggatctgggt gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg
    49741 ttacgctttc catgtgacaa tgttgaataa cgggggtctg atagtgcccc tgtacctggt
    49801 agtcggcggc atcgattgtt tgttggacat tacccgtcga catcgtgctt tgtttcagta
    49861 aattgccacc gttatggatt ggtgcagcgt cttctgccag cgccgcttct ggcgtggtga
    49921 taacgggtaa ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt
    49981 catcgcgggc cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca
    50041 gtgcgcgatc ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagcagtag
    50101 caaatggaat atcaggcaca tcttcccagg taaaaatcgc cagtacgccc ggcaaacttc
    50161 tggcttgttc atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt
    50221 tcgcataaca catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct
    50281 tagcaatggc atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca
    50341 tagaaatccc ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca
    50401 agatttacgc cagaaacgac gataagcgtt aggggaggta ggaagtgtga tgacgttaaa
    50461 aaaacacaga tatgatacga aacaggactc tgaatagggg cagtgatacg tatcaatatg
    50521 agtggcgata aaatcaatgt gataaattgg gattattacg catcaggatg ggaaataggt
    50581 taaaaagtca ggaagttcga tacctctaca gcgaatattt tgctgatgat acttcccttt
    50641 gccaaatgaa attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt
    50701 atcaagaggc tggctgctat gataaggcgt cttgtttttt aagcgaggaa agattttgag
    50761 tgcgggacgc ctgaataaaa aatctctggg tatcgtgatg ttgttatcgg ttggactgct
    50821 tttggcgggc tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta
    50881 caccgtgaaa cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa
    50941 agaactggcg cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact
    51001 aaaactgggt ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac
    51061 caaaaccgca tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg
    51121 gcaacgttgt tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga
    51181 tggcggcaat aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg
    51241 tgcaggaaag gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat
    51301 taaacacagt gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa
    51361 tgggcaaagc gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc
    51421 tgttcgcctg catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt
    51481 gccgccgcag ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg
    51541 gcacttgcta aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt
    51601 tcaatggtag aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct
    51661 ccaatttatc ttcacctgat caatctgcca tttttgctgt atctgatttt ctatattatc
    51721 ttaattcaat cgcttcagac ttttcgcaat cgccgtaata cttccccatt ttgttgtaaa
    51781 taaaaagcgt ttacgcaatg caatcctttt gtaacaaccc aggtaaaaca ccttattttc
    51841 aagttgttaa ataagtggtt atctacgctt ttaacaaacg attgatggaa tattttatga
    51901 aagggaaatc tgcattaact cttctgctcg ctggtatatt ttcgtgcggc acatgccagg
    51961 cgacaggggc agaagtgacg agtgaatctg tatttaacat tcttaattcc acgggggcag
    52021 caacagataa aagttattta tcgttaaatc cagataagta tcctaattat cgtcttttga
    52081 ttcactcagc caaattacaa aatgaaataa agagccacta taccaaagac gaaattcaag
    52141 gattgctaac attaacagaa aacacacgaa agttaacgtt gacggagaaa ccctggggaa
    52201 cgtttattct tgcttctact ttcgaagatg acaaaacagc ggcagaaacc cactatgatg
    52261 ctgtctggct gagggatagt ttatggggct atatggcatt agtatcagat cagggaaata
    52321 gtgtagccgc aaaaaaggtt ctgctcaccc tgtgggatta tatgtctacg cccgaccaga
    52381 ttaagcggat gcaggacgtc attagcaacc ccaaacgact ggatggagtt cccgggcaaa
    52441 tgaatgttgt gcatatccga tttgacagca actcccccgt gatggcagat gtgcaggaag
    52501 aaggcaagcc gcagctgtgg aaccataaac aaaatgatgc gttaggtctt tatcttgatc
    52561 tccttataca agcaatcgac actgatacaa ttaacgctga agattggcaa aaaggtgacc
    52621 gattaaaatc ggttgcgctg ctgattgctt atctggataa agccaatttc tatgttatga
    52681 aagattccgg agcatgggaa gaagatgccc gattaaacac atcttccgtt gcactggtga
    52741 cctctggact ggaacgtttg tcgaacttac tctcgaagaa agactctgtt tttgtctctg
    52801 atttactacg cgaagctaaa gccaatgaac tggatgaacc tctttcaaca acgaggttga
    52861 accacctcat agataaaggt tatgaaagaa taacgttaca gcttgattta gggggagaat
    52921 ccccaggtta tctggaaaaa gataaacatt acagagaagc cgatgcggct ttacttaatg
    52981 ttatttatcc tgcaaatctt gcaaagataa acaccagaag aaaagagcag gttctaaaaa
    53041 tagttaaaaa actagcaggt ccgtatggta taaaaagata cgagaaggat aattatcagt
    53101 cggcaaattt ttggttcaat gatattaaaa ccgataccga tcaaaattca catgcgaaaa
    53161 gagaaaagag cttcattcca tcaacagaag cagaatggtt ttttgactca tggtatgcaa
    53221 aatcagcagc aattgtttat aaagaatcaa gaaaggaaga gtatttgaat gactcagtgc
    53281 aatttatgaa tagatcattg gcccaaataa caggcgagaa tatgattggt gcaaatggca
    53341 ggagtgtacc tgaaatggca cttcccgaaa gctataacta tatccacaag tcgggaactt
    53401 tgcatgaagc gccaagccct atcattccgc taaattggtc gaaagcttca atgactctca
    53461 tgttgaaaga gatgtccaac cttatcaatg atgagggaat taaatagttt tcataatgag
    53521 ttacagcaca acgttgggta ttgactgagc aagatgaacg acaaggacaa cgacatcagc
    53581 cttatgagga gcaagaaaat gaaagcaaat tcgaaaacga tcagaaggat gaaagttaat
    53641 gttcggtctg aggaaagtaa atcgaaacac tcatacgttt gaaaccactt ttcaaaattg
    53701 gaaagaaaat ggcgaagatg ttgcattatt aatgccagaa tttagtgcta aatggttacc
    53761 gatagcagaa gagagtggca gctggtctgg atgggtattg cttggagaaa tatttccctt
    53821 aatttcatct gagctcgctg ggatggcttt aatgccagaa acggaaaggt taatagggga
    53881 atggctcagt ttatccagtt ccccgttaaa tctaaagcat cctgaactaa aatataatcg
    53941 tttgcacgta ggtaaagtat ttgatggagt attgagccct gctcaaccac taataagaat
    54001 atgaacaggg gaattgaatc tttggttaga taaagtcaca gtctgccaat acggaaacgc
    54061 tccagtgtta gacaaaaaat cattaaattg gtccattcat tttgtaattg ga
//
LOCUS       DAPHDX010000010        52595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPHDX010000010 DAPHDX010000000
VERSION     DAPHDX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.010"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            248..400
                     /gene="mokC"
                     /locus_tag="RH058_000602"
     CDS             248..400
                     /gene="mokC"
                     /locus_tag="RH058_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV3136239.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(504..1634)
                     /gene="dnaJ"
                     /locus_tag="RH058_000603"
     CDS             complement(504..1634)
                     /gene="dnaJ"
                     /locus_tag="RH058_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV3136240.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1723..3639)
                     /gene="dnaK"
                     /locus_tag="RH058_000604"
     CDS             complement(1723..3639)
                     /gene="dnaK"
                     /locus_tag="RH058_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV3136241.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4016..4420
                     /locus_tag="RH058_000605"
     CDS             4016..4420
                     /locus_tag="RH058_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV3136242.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKERQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4446..5159
                     /gene="msyB"
                     /locus_tag="RH058_000606"
     CDS             4446..5159
                     /gene="msyB"
                     /locus_tag="RH058_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV3136243.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5308..5874
                     /gene="satP"
                     /locus_tag="RH058_000607"
     CDS             5308..5874
                     /gene="satP"
                     /locus_tag="RH058_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV3136244.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5909..6496)
                     /gene="mog"
                     /locus_tag="RH058_000608"
     CDS             complement(5909..6496)
                     /gene="mog"
                     /locus_tag="RH058_000608"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV3136245.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6611..7564)
                     /gene="tal"
                     /locus_tag="RH058_000609"
     CDS             complement(6611..7564)
                     /gene="tal"
                     /locus_tag="RH058_000609"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV3136246.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7843..9273
                     /locus_tag="RH058_000610"
     CDS             7843..9273
                     /locus_tag="RH058_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV3136247.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9343..10119
                     /gene="yaaA"
                     /locus_tag="RH058_000611"
     CDS             9343..10119
                     /gene="yaaA"
                     /locus_tag="RH058_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV3136248.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10272..10568)
                     /locus_tag="RH058_000612"
     CDS             complement(10272..10568)
                     /locus_tag="RH058_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV3136249.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10782..12068)
                     /gene="thrC"
                     /locus_tag="RH058_000613"
     CDS             complement(10782..12068)
                     /gene="thrC"
                     /locus_tag="RH058_000613"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV3136250.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12069..13001)
                     /gene="thrB"
                     /locus_tag="RH058_000614"
     CDS             complement(12069..13001)
                     /gene="thrB"
                     /locus_tag="RH058_000614"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV3136251.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     GLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13003..15465)
                     /gene="thrA"
                     /locus_tag="RH058_000615"
     CDS             complement(13003..15465)
                     /gene="thrA"
                     /locus_tag="RH058_000615"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV3136252.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15491..15610)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15546..15611)
                     /gene="thrL"
                     /locus_tag="RH058_000616"
     CDS             complement(15546..15611)
                     /gene="thrL"
                     /locus_tag="RH058_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV3136253.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15825..16511)
                     /locus_tag="RH058_000617"
     CDS             complement(15825..16511)
                     /locus_tag="RH058_000617"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV3136254.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16911..17051)
                     /locus_tag="RH058_000618"
     CDS             complement(16911..17051)
                     /locus_tag="RH058_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136255.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17147..17863
                     /gene="arcA"
                     /locus_tag="RH058_000619"
     CDS             17147..17863
                     /gene="arcA"
                     /locus_tag="RH058_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV3136256.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17923..19275)
                     /gene="creD"
                     /locus_tag="RH058_000620"
     CDS             complement(17923..19275)
                     /gene="creD"
                     /locus_tag="RH058_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV3136257.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(19333..20757)
                     /gene="creC"
                     /locus_tag="RH058_000621"
     CDS             complement(19333..20757)
                     /gene="creC"
                     /locus_tag="RH058_000621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV3136258.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20757..21446)
                     /gene="creB"
                     /locus_tag="RH058_000622"
     CDS             complement(20757..21446)
                     /gene="creB"
                     /locus_tag="RH058_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV3136259.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVIILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21459..21932)
                     /gene="creA"
                     /locus_tag="RH058_000623"
     CDS             complement(21459..21932)
                     /gene="creA"
                     /locus_tag="RH058_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV3136260.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22143..23012
                     /gene="robA"
                     /locus_tag="RH058_000624"
     CDS             22143..23012
                     /gene="robA"
                     /locus_tag="RH058_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV3136261.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23009..23656)
                     /gene="gpmB"
                     /locus_tag="RH058_000625"
     CDS             complement(23009..23656)
                     /gene="gpmB"
                     /locus_tag="RH058_000625"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV3136262.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23708..24229
                     /gene="yjjX"
                     /locus_tag="RH058_000626"
     CDS             23708..24229
                     /gene="yjjX"
                     /locus_tag="RH058_000626"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV3136263.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAWKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24314..24640)
                     /gene="trpR"
                     /locus_tag="RH058_000627"
     CDS             complement(24314..24640)
                     /gene="trpR"
                     /locus_tag="RH058_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV3136264.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24730..26667)
                     /gene="sltY"
                     /locus_tag="RH058_000628"
     CDS             complement(24730..26667)
                     /gene="sltY"
                     /locus_tag="RH058_000628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV3136265.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFVRTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26878..28545
                     /gene="ettA"
                     /locus_tag="RH058_000629"
     CDS             26878..28545
                     /gene="ettA"
                     /locus_tag="RH058_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV3136266.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28852..30084)
                     /gene="nadR"
                     /locus_tag="RH058_000630"
     CDS             complement(28852..30084)
                     /gene="nadR"
                     /locus_tag="RH058_000630"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV3136267.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30105..31487)
                     /gene="radA"
                     /locus_tag="RH058_000631"
     CDS             complement(30105..31487)
                     /gene="radA"
                     /locus_tag="RH058_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV3136268.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31536..32504)
                     /gene="serB"
                     /locus_tag="RH058_000632"
     CDS             complement(31536..32504)
                     /gene="serB"
                     /locus_tag="RH058_000632"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV3136269.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32610..33254
                     /locus_tag="RH058_000633"
     CDS             32610..33254
                     /locus_tag="RH058_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV3136270.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33282..34298
                     /gene="lplA"
                     /locus_tag="RH058_000634"
     CDS             33282..34298
                     /gene="lplA"
                     /locus_tag="RH058_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV3136271.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34330..34479
                     /locus_tag="RH058_000635"
     CDS             34330..34479
                     /locus_tag="RH058_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136272.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34754..35473)
                     /gene="deoD"
                     /locus_tag="RH058_000636"
     CDS             complement(34754..35473)
                     /gene="deoD"
                     /locus_tag="RH058_000636"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV3136273.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35553..36776)
                     /gene="deoB"
                     /locus_tag="RH058_000637"
     CDS             complement(35553..36776)
                     /gene="deoB"
                     /locus_tag="RH058_000637"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV3136274.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36828..38150)
                     /gene="deoA"
                     /locus_tag="RH058_000638"
     CDS             complement(36828..38150)
                     /gene="deoA"
                     /locus_tag="RH058_000638"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV3136275.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38277..39056)
                     /gene="deoC"
                     /locus_tag="RH058_000639"
     CDS             complement(38277..39056)
                     /gene="deoC"
                     /locus_tag="RH058_000639"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV3136276.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39314..40864
                     /locus_tag="RH058_000640"
     CDS             39314..40864
                     /locus_tag="RH058_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV3136277.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40836..41699
                     /locus_tag="RH058_000641"
     CDS             40836..41699
                     /locus_tag="RH058_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV3136278.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41812..42594)
                     /locus_tag="RH058_000642"
     CDS             complement(41812..42594)
                     /locus_tag="RH058_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3136279.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(42591..43664)
                     /locus_tag="RH058_000643"
     CDS             complement(42591..43664)
                     /locus_tag="RH058_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV3136280.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43786..43947)
                     /locus_tag="RH058_000644"
     CDS             complement(43786..43947)
                     /locus_tag="RH058_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV3136281.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44074..44679)
                     /gene="osmY"
                     /locus_tag="RH058_000645"
     CDS             complement(44074..44679)
                     /gene="osmY"
                     /locus_tag="RH058_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV3136282.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45072..46661)
                     /gene="prfC"
                     /locus_tag="RH058_000646"
     CDS             complement(45072..46661)
                     /gene="prfC"
                     /locus_tag="RH058_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV3136283.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46752..47429)
                     /gene="yjjG"
                     /locus_tag="RH058_000647"
     CDS             complement(46752..47429)
                     /gene="yjjG"
                     /locus_tag="RH058_000647"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV3136284.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47444..47890)
                     /gene="rimI"
                     /locus_tag="RH058_000648"
     CDS             complement(47444..47890)
                     /gene="rimI"
                     /locus_tag="RH058_000648"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV3136285.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47859..48272)
                     /gene="holD"
                     /locus_tag="RH058_000649"
     CDS             complement(47859..48272)
                     /gene="holD"
                     /locus_tag="RH058_000649"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV3136286.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48375..49406
                     /gene="rsmC"
                     /locus_tag="RH058_000650"
     CDS             48375..49406
                     /gene="rsmC"
                     /locus_tag="RH058_000650"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV3136287.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            49674..49760
                     /locus_tag="RH058_000651"
     tRNA            49674..49760
                     /locus_tag="RH058_000651"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49708..49710,aa:Leu,seq:cag)
     gene            complement(49799..50035)
                     /locus_tag="RH058_000652"
     CDS             complement(49799..50035)
                     /locus_tag="RH058_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV3136288.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            50176..50964
                     /gene="fhuF"
                     /locus_tag="RH058_000653"
     CDS             50176..50964
                     /gene="fhuF"
                     /locus_tag="RH058_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV3136289.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(51002..51679)
                     /gene="bglJ"
                     /locus_tag="RH058_000654"
     CDS             complement(51002..51679)
                     /gene="bglJ"
                     /locus_tag="RH058_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV3136290.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(51637..52362)
                     /locus_tag="RH058_000655"
     CDS             complement(51637..52362)
                     /locus_tag="RH058_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV3136291.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        12432 a        14207 c        13198 g        12758 t
ORIGIN      
        1 ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt cttcaggatt agaatcgata
       61 aaacagaatg ggttaaagag agcgatataa tagcggcggg tgcttgaggc tatctgtctc
      121 aggcattagc tgaacggcag atagagaaaa gccccgagtg atattttact atcaacccga
      181 ggccccctat atgctgaaca catgtagagt gcctcttact gaccgtaagg tcaaggagaa
      241 gagagcaatg aagcagcata aggcgatgat tgtcgccctg atcgtcatct gtatcaccgc
      301 cgtagtagcg gcgctggtaa cgagaaaaga cctctgtgag gttcacatcc gaactggcca
      361 gacggaggtt gctgttttca cggcttacga atccgagtaa gagcaacggc ggggagtgat
      421 ccccgccact ttacaggtgc ttgcatatct tcaacgcacc ctggttttac ccaggcctgc
      481 ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt caaaaaactt cttcacacca
      541 tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg ttgggccacc gaagctttct
      601 tgcagctctt gcagcaactg tttctgcttc tcgttcaggc ctaccggtgt ttcgacgaca
      661 acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag acttgacgcc tttaccgcgc
      721 atacggaaaa gcttaccggt ctgggtttcg ccaggcactt tcagtttgac gcgaccatca
      781 agggtcggta cttcgatttc gccacccagc gccgccatag cgaagttgat cgggacttcg
      841 caatagaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg aacctgaacg
      901 tacagatcgc ctgccggtgc gccatgctcg cccgcttcac cttcgcccgc aagacggatg
      961 cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct gcgctcaaca
     1021 cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg gccctgacag
     1081 tgtggacagg tctgctgtac agcgaagaat ccctgacgca tctgcacctg accagaacca
     1141 tgacaggtcg gacaggtctg cggctgtgta cctggttttg caccgctacc gtggcaaacg
     1201 tcacactctt ccagagtcgg aatgcggatc tctttggtca cgccacgtac agcttcttcg
     1261 agggtgagct ccatgttata gcgtaaatca gcaccgcgcg ccgcacgttg acgaccacgt
     1321 ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat cgctgaagtc tgcgccgccg
     1381 ccaaaaccgc cgccgcccat accaccttgc tcaaacgcag catgaccata ctgatcgtat
     1441 gccgcacgtt tttgcgagtc ggtcagaact tcataagctt ccttgatctc tttaaatttc
     1501 gcctcggcct ctttgtcacc ctggttacgg tccgggtggt atttcatggc caggcgtttg
     1561 taggcctttt tgatttcacg ctcttccgct gttttggaaa cgcctaaaat ctcgtaataa
     1621 tcttgcttag ccatcttttt taaattgccc ctagatgaat gcacgggcgg agaggaaatt
     1681 ccccttcgcc cgtgtcagta taattacccg tttatagggc gattattttt tgtctttgac
     1741 ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg ttgtttgcag aagcatcagc
     1801 accggcagtc tgctgctggg catgttgctg ctgggcgatt tccatcagtt tctgggaaac
     1861 ctgtgccagc tcctgcattt tcgcttcgat agcggctttg tcttcacctt tcagagcagt
     1921 ttccagtgca gtcagtgcag actcgatagc agttttgtcg tcagccggca gtttgtcgcc
     1981 tgcttcttca acctgcttac gggtgctgtg cagcagatgg tcgccctggt tgcgagtctg
     2041 taccagctct tcaaacttac ggtcagcttc ggcgttagct tctgcgtcgc gtaccatttt
     2101 ctggatttca tcttcgttca gaccagaaga agccttgatg gtgatcttct gctctttacc
     2161 gctgttttta tctttcgcgg aaacgtgcag gataccgtca gcatcgatat cgaaggtaac
     2221 ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca tccaggttga actgacccag
     2281 agatttgtta tcagccgcac gtttacgttc accctgcagc acatggatgg ttaccgcaga
     2341 ctggttgtct tcagcggtag agaacacctg gctgtgcttg gtcgggatag tggtgttttt
     2401 cgcgatcagc gtcgtcatca caccgcccat ggtttcgata cccagagaca gcggggtaac
     2461 gtccagcagc agtacgtctt tcacgtcacc agtcagaaca ccaccctgaa cagcagcacc
     2521 gattgctaca gcttcgtccg ggttaacgtc tttacgcggc tctttaccaa agaactcagc
     2581 aactttcttc tgaaccattg gcatacgagt ctgaccacca acgaggataa cgtcgtcgat
     2641 atcagatacg gacaggccag cgtcctgcag tgcaactttc agcggctcaa tggaacggtt
     2701 taccagatct tcaaccaggc tttccagttt cgcacgagtc actttgatgt tcatgtgttt
     2761 cggaccggtc gcatctgcag tgatgtacgg caggttaacg tcggtctgct gagcggaaga
     2821 cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc tgcattgcca gcggatcgtt
     2881 gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc aggtagttga tcagacggct
     2941 gtcgaagtct tcaccaccca ggtgggtatc accgttggta gccagaactt cgaaggtttt
     3001 ttcgccgtca acttcgtcga tttcgataat agaaatatcg aaagtaccac cacccaggtc
     3061 ataaaccgcg atagtacggt tgccagtacc tttgtccaga ccgtaagcca gcgcagctgc
     3121 ggtcggttcg ttgatgatac gttttacttc cagaccagcg atacggcctg cgtctttggt
     3181 tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg ataacagctt cagttaccgg
     3241 ttcacccaag taatcttcag cggttttctt catttttttc agcacttcag cagagatctg
     3301 cggcggtgcc attttctggc ctttaacttc gacccatgcg tcgccgttat cagcagcaat
     3361 aattttgaac ggcatgatgg aaacatcacg ctgtacttct tcgtcctgga agcggcggcc
     3421 aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc gtcactgcct gacgtttagc
     3481 cggctgacca accagagttt caccatcctg ggtataggca atgatagaag gcgtggtgcg
     3541 atcgccttcg gcgttctcca gcacgcgagg agtggtgcca tccataatcg ctacacaaga
     3601 gttggtagta cccaggtcga taccaattat tttacccatc taaacgtctc cactatatat
     3661 tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa cgtctggttt caactgccca
     3721 atttcatttt tttgcagact cactcactgc ggttgactac taaatggggt cgtaacccac
     3781 gtcatcaagg gggagatgca aaaatttttt gtgcttttgc gcagaaattt cgcgatatag
     3841 caggacaaga cgtgaaactg acagcaattt tattcatata aagaatgaat cgcagccaag
     3901 acattcatca aaaaattgta ataaaaagaa aacattacgc acctgaatct tctctttatc
     3961 agcagtaaac tagtgggtat ccatccccct acctctcccc actaagagaa tccttatgaa
     4021 atccgttttt acgatttccg ccagcctggc gattagtctg atgctgtgct gcacggcgca
     4081 ggcaaacgac cataaaatcc tcggcgtcat tgcaatgccg cgtaacgaaa ccaacgatct
     4141 ggcgttgaaa cttcctgttt gccgaattgt taaacgcata caactctctg ccgaccatgg
     4201 cgatttacag ttaagcggtg catcggttta tttcaaagcc gcccgtagcg ccagtcagag
     4261 cctgaatatt ccttcagaaa taaaagaaag gcaaaccact gactggatca acattaacag
     4321 cgataacgac aataaacgct gcgtctcaaa aatcaccttt tccggtcata cggtgaactc
     4381 atcggatatg gccacgctga aaattatcgg cgacgactaa cgccagaata tagaagccac
     4441 aaaaaatgaa tgttaattac ctgaatgatt cagatctgga ttttctccag cattgcagtg
     4501 aggaacagtt ggcaaatttc gcccgattgc tcacccataa tgaaaaaggc aaaactcgcc
     4561 tctccagcgt actgatgcgc aacgaactgt ttaaatcgat ggaagggcat cccgagcaac
     4621 atcgccgcaa ctggcagctg attgctggag aattacagca ttttggtggc gatagtatcg
     4681 ccaacaaact gcgcggacac ggtaaattgt atcgggccat tttgctcgat gtttcaaaac
     4741 gattgaagct gaaagccgac aaagagatgt ctacgtttga gattgagcaa cagttactgg
     4801 aacaatttct gcgtaacacc tggaagaata tggacgagga acataagcag gagtttctgc
     4861 acgcgatcga tgccagggtg aatgagctgg aagagctgct gccgctgctg atgaaagaca
     4921 aattattggc aaaaggcgtg tcgcatttgc tttccagcca actgacccgc attttacgca
     4981 cccacgcagc aatgagcgta cttgggcatg gtttgctgcg cggcgcgggg ctgggaggcc
     5041 ctgtaggtgc ggcactaaat ggggttaaag cggtcagcgg cagcgcctat cgcgtgacga
     5101 ttccagccgt actgcaaatc gcctgcctgc gccggatggt tagcgccact caggtctgat
     5161 accggaaacc ggggaaaatt ttcattttcc cctcaaaaga tcgtagacac tgccccactg
     5221 gctgattatt atgccgcgcc ctgaaaacac tacagttatt cagggaaatt atttcaccat
     5281 tcattcgatg atgatttttg aggaattatg ggcaacacta agttggctaa tccggcaccg
     5341 ctgggcctga tgggcttcgg catgaccacc attctgctta acctgcacaa cgtgggttat
     5401 ttcgctctgg acggtattat tcttgccatg ggcattttct acggcggcat cgcgcaaatt
     5461 tttgctggtc tgctggagta caaaaaaggc aacactttcg gtttaaccgc attcacctct
     5521 tacggttctt tctggctgac gctggttgcg attctgctga tgccgaaact gggtctgacc
     5581 gatgcgccaa atgcacagtt ccttggtgtc tatctgggtc tgtggggcgt atttacgctg
     5641 tttatgttct tcggcacgct gaaaggcgca cgcgttctgc aattcgtttt ctttagcctg
     5701 accgtgctgt ttgccctgct ggcgatcggt aacattgccg gtaacgccgc aatcatccac
     5761 tttgccggct ggattgggct gatctgcggt gccagcgcaa tctatctggc gatgggtgaa
     5821 gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg gtgaatccca ctaattgttt
     5881 tgagatcccc ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg
     5941 gtctgaatgc tgcaaccact tccggtgccg tttcaacgta tggcccttcc agcaactgaa
     6001 tgcagtacgg tacgctggca aaaataccgt gtaccacaac gttacccgca gcgtccttca
     6061 caccttccag cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct
     6121 gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctta
     6181 tctggcgcat ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat
     6241 cgggcgttac gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac
     6301 tcatttcatc cactagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta
     6361 agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgcag
     6421 ggatgccttt atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa
     6481 tacgtaaagt attcatcaag atttttccgt ttaaacagtc tcgttaaaca gaatgataca
     6541 ctgcgaaggg agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg
     6601 ctaagaatga ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa
     6661 acttacggat accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca
     6721 ggaactcgga ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt
     6781 tacgttcgat agccccttcg ctctcagcca gctctttcag cagtgtaggt gcgatggtaa
     6841 gacggtcgca gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa
     6901 ccacggtttc ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc
     6961 ccggatcttc tgccggagcg tactctttct tatcagtatt cgctttgtac cagtcaagaa
     7021 tacggccaac aaatggcgag atcaggaaca cgcctgcttc cgcacaagca cgagcctgag
     7081 cgaaggagaa cagcagggtc aggttacagt tgatgccttc tttttccagc tgttctgcag
     7141 cacggatacc ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaataccag
     7201 catcgttgta gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg
     7261 aaagacgcgc atcaacttca gttgagatac gacccggaac cagtttcagg atttccagac
     7321 caatatttac tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct
     7381 gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg
     7441 cgttaagaat gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg
     7501 cgatgtcccc agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg
     7561 tcatgatagt atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg
     7621 atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt
     7681 ttcggatttc tgacactgcg ttgatatcgc ccgccatttt tatacaaaac ctcatgtatg
     7741 ctacgcagaa gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt
     7801 tacacaggaa agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc
     7861 tttattaaca gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt
     7921 tggttcactt ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt
     7981 cttaaaaata gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc
     8041 agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc
     8101 ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt ttatcggcat ggcgacctcg
     8161 tttgccgaat gttctctggc acaactttat aaagaacgtg acgtcaatgg acagtttcgt
     8221 ggcggaccgg catggtatat ggcgcgcggg ctggggatgc gttggatggg cgttctgttc
     8281 gccgtctttt tgctcatcgc ctatggcata attttcagcg gagttcaggc gaacgccgtt
     8341 gcccgcgccc taagtttttc ttttgatttt cctccgctgg tgacaggcat tattctcgct
     8401 gtctttgctc tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg
     8461 tttgtcccgt tgatggcgat aatctgggtg ctgaccagca tggtgatttg cgtaataaat
     8521 atcgggcaac ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa
     8581 gcggcaggcg gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc
     8641 agtatgtttt ccaatgaggc gggaatgggt tcgacgccaa acgcggcagc ggcagcggcg
     8701 tcctggcctc cgcatccggc agcgcaaggg attgtccaga tgattggcat ttttatcgac
     8761 accctggtca tctgtacggc aagcgccatg ctgatattac tggcgggtaa cgacacaacc
     8821 tacatgccgc tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc
     8881 tggggtgctg agtttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc
     8941 aactacattt atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc
     9001 tggtgtttgc ggatctgcac cttcgcaacg gtcatcggcg gcaccttgct aagccttccg
     9061 ctgatgtggc aactggcaga tatcataatg gcctgcatgg ctattaccaa tttgaccgcc
     9121 attttactgc tctcgcctgt ggttcatacc attgccagtg attatctacg ccagcgtaaa
     9181 ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg atatcggtcg ccagctttct
     9241 ccggacgcgt gggatgatgt ttcgcaggag taatcacaac tatcgatcaa ctcattctct
     9301 ttttttgcta aagtcggcat aaatttcctg caaggactgg atatgctgat tcttatttca
     9361 cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca cgcgctatac gctgccggag
     9421 ctgttagaca attcccagca gttgatccat gaagcgcgga aactgacgcc tccgcagatt
     9481 agcacgctga tgcgcatcag cgacaaactg gcgggtatca acgccgctcg ctttcatgac
     9541 tggcagccag atttcacgcc ggagaatgcc cgccaggcga ttctggcgtt taaaggtgat
     9601 gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg atttcgattt tgcccaacag
     9661 catttgcgaa tgctttccgg cttgtatggc gtactgcgcc cgctcgattt aatgcagcct
     9721 tatcgtctgg aaatggggat ccgtcttgag aatgcccgag ggaaagatct gtatcaattc
     9781 tggggagata tcatcaccaa caagctgaac gaggcgctcg cagcacaagg cgataatgtg
     9841 gtgattaatc tggcgtcaga tgagtatttt aaatcggtga agccgaagaa attgaatgcc
     9901 gagattatca agccggtgtt cctcgatgag aagaacggca aatttaagat aatcagcttc
     9961 tacgctaaga aagcacgcgg tctgatgagt cgttttatta ttgaaaatcg gctgaccaaa
    10021 ccagagcaac tgactggttt taatagcgaa ggttacttct ttgatgaaga ttcctccagc
    10081 aatggcgaac tggtgtttaa acgctacgag cagcgttaag tttgtagtca atgccggatg
    10141 cggcgtgaac gccttatccg gcctacaaaa gcatgcagat tcaatatatt gcagaattca
    10201 cgtaggcctg ataagacgcg ccagcgtcgc atcaggtgct gaatgccgga ttgttacccg
    10261 gcatttgtca tttagcggtg atgtttgcct ggaccatgac cgccgtgatg atcatgagga
    10321 gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg ggtgccagcg attgcctcgc
    10381 cattcataat gttgtttcca ccagccgtgg tcgcgccagt gacctccatc ccagtaatag
    10441 ccacgattat cacgatcgcc tatctgtaat tttactgacg ggactaacgt aatttccgca
    10501 gcctgtgctg ccatgggagc gaccagaacc agggaaagtg cgagtacgat agattgcatc
    10561 tttttcacag gttactcctt ttatcattgc ccgttatggg tcgatatccg gagattacgc
    10621 gatgagaacg acggttgtta ttctccgcaa tcctaatctc taagttaccg catttattga
    10681 gaatttctcc tttttccctg tcgtttttct ccataatttc ttcataaaaa agccgggctg
    10741 cataaaagca aacccggcct gattgagata atgaatagat tttactgatg attcatcatc
    10801 aatttacgca acgcagcaaa atcggcgggc agattatgtg aaagcaaggg taaatcagca
    10861 cgttctgcca gctcttttgg cagatccaac gtttcaccga gaatcgcttc cacgctctct
    10921 ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat attcgcctgg attcaactgg
    10981 tcacgcagcg cacgataagc tacggcggcg tgcggctccg aggtgtagcc cagttctttt
    11041 aactcacgca ttgtctgttg cgtggtttca tcatccacgg ctgcataacc cagctctttc
    11101 agttgccaga ttttgcggcg gaacaactct tccacacgcg gccagttgtt cggctggctc
    11161 acgtccatcg cgttggataa cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc
    11221 aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa taaaacgttt caccggcaga
    11281 ccgagtgact tcgccagcag acccgccgtc aaatcgccga agtttccgct tggcaccgag
    11341 acaaccagct ggttgcgcgc ttcctgcggc agctgcgcaa ctgcttcaaa gtagtagcaa
    11401 atctgcgcca gcaaacggct gatgttaatc gagttagctg agtttaaccc cagcgccact
    11461 ttcagctctt catcatcaaa cgcctgcttc accagcgcct gacaggcatc gaaatcgccg
    11521 tcgatggcaa cagtttcgat attgccgccc aatgtacaga acagtttttc ttgcagtgga
    11581 ctgattttgc ctcgtggata gaggataacc actttcacat tcggtaaacc gtagaaagca
    11641 tgagctactg ccgctccggt atcaccggag gtcgcggtca gaatggtcac tggcttatcg
    11701 cccgcaatat gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc
    11761 gttggcccgt ggaacaattc cagacaaccg acatcgcttt caacattggc gaccggagcc
    11821 gggaaggcaa acgccgcgcg cacgcgctct tccaggattt cctgcgggat ttcatcacca
    11881 ataaacgccg agaggatctt cgcactgcgg gtgacaaaat ccagcttcag catctcatca
    11941 atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa acagcccctg atttttgccc
    12001 aacccctggg ttacggcttg cgcaaagctg acctgctcat tgtgatcttt cagattgtag
    12061 agtttcattt agttttccag tactcgtgcg cccgccgtat ccagccggca aatatgaaca
    12121 aaaccttcct gattttgcag gtagttctta cccaaccagt cggcaacgcg ctgggcggta
    12181 tccggcttgt cacacagagc gaacaaggtc gggccggagc cggagatacc gctcgctacc
    12241 gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc caggcagtaa ccgttcacgg
    12301 tagggttctg cgataacatc tttcatcagc ttcgcggcaa gctcaggctg acgggaatag
    12361 caggcgtgaa tgaagcctgc cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac
    12421 tgcgccggta aaatagcccg ggcttctgcc gtcgagactt taatccccgg atacgccagc
    12481 acccacagcc actcatcaaa ccctggcact tgctggctga tgatgtcgtt ttcttcgatc
    12541 atcaactgca ttccaccaag aaaacacggt gccacgttgt cgtaatgaat gctgccggag
    12601 atacgtcctt ccagctcgcc catcaaagcc agcaaacggg tgtcattaag tggcttgccg
    12661 cagtgttcat tcatcgccat cagcgccgcg accaccgaac aggcgctgga gcctaagccc
    12721 gagccgatcg gcatattctt ttccagagtc atcgccactg gaatttgctt cccaagctcc
    12781 tggcaaaaac gctcccagca ctgataaacg atattttccc gtggttctga cggcagctta
    12841 tcggcaaagc gtccgaggtt gttgagaccg aatgtctctg ccgcctcaac cgtgactaca
    12901 tctccgagca atgcaccatc aacgggtgtc accgccgccc cgagcacatc aaacccgacg
    12961 ctcatattgg cactggaagc cggggcataa actttaacca tgtcagactc ctaacttcca
    13021 tgagagggta cgtagcagat cggcaaagac accggcagcg gtaacgtcat tgcccgcacc
    13081 atatccgcgc agcaccaacg gcagcggctg ataatagtgg ctatagaagg ccagggcgtt
    13141 ttcgccattt ttcactttga acagcggatc attaccatcc acttcggcaa tcttcacgcg
    13201 gcagacgcca tcttcatcaa tattgccaac atagcgcaaa acttttcctt catcacgggc
    13261 cttagccacg cgcgcggcaa agagatcgtc gagctgtgac agattcgcca taaaagcggc
    13321 aacatcaccc tcggcgttaa actctgcggg cagcactggt tcaatttcaa tatccgccag
    13381 ctccagttca cgtcccgttt cacgagcgag aatcaatagc ttacgcgcta catccatacc
    13441 agaaagatca tctcgcggat ccggttcggt ataacccatt tcccgcgcca gagtagtcgc
    13501 ctcggagaaa ctcatgcctt cgtctaactt gccgaagata taagaaagcg aacctgaaag
    13561 aatgccggag aacttcatca attcatcacc agcattgagc agattttgca ggttctcaat
    13621 aaccggtaat ccagccccaa cgttggtgtc atagaggaat ttacgccgcg atttttccgc
    13681 cgcatgacgc aacagatggt agtaatccat cgacgaggtg ttggcctttt tgttcggcgt
    13741 gacaacgtgg aaaccttcgc gcaagaagtc ggcatattga tccgccactg cctggctgga
    13801 ggtgcagtca acaatgaccg ggttcagcag atgatattct ttcacgaggc gaattaagcg
    13861 cccgagatta aacggctctt tggcttgcgc cagttcttcc tgccagtttt ccagatttag
    13921 gccatgcaca ttggtgagca gtgccttcga gttggcaaca ccgcagacac gtaagtcgat
    13981 atgtttattc ttcagccagc tttgttgacg cttcagttgc tccagcagcg caccgccaac
    14041 gccaccgacg ccaatcacaa acacttcgat aacctgatcg gtattgaaca gcatctgatg
    14101 agtaacgcgc acgccagtgg tcgcatcatc gttatttacc acgacagaga ttgagcgttc
    14161 agaagatccc tgagcaatgg cgacaatgtt gatattggcg cgggccagcg cggcaaagaa
    14221 tttcgccgag atcccacgca aggtgcgcat accatcacct accaccgaga taatggccag
    14281 ccgttccgtc accgccagcg gctccagtaa gccttctttc agttccaggt agaactcttc
    14341 ctgcattgcc cgttcagccc gcacacagtc gctttgcgga acgcagaaac tgatactgta
    14401 ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca tcgcggcaaa
    14461 gacgcgcgcc gccatgccga ccattccttt catccccggg ccggaaacgc tgaacattgc
    14521 catgttattc agattggaaa tgcccttgac cggtaattcg tcttcatcac gactggcacc
    14581 aatgagcgta cctggtgctt gaggatttcc ggtattttta atcaggcaag ggatctggaa
    14641 ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta gcgccgaagt aggaaagctc
    14701 catcgcttcc tggtaggaca tcgacttcaa caacctcgca tcgggcacct gacgcgggtc
    14761 gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc
    14821 agccagcacc gccgcggagt agtcggaacc gttgcgtcca agtaccacca gttcgccttt
    14881 ttcattaccg gcggtgaaac ctgccatcaa caccatgtga tcagccggaa tgcggcttgc
    14941 cgcaatacgg cgggtggact cagcaatatc gacggtagat tcgaggtaat gccccactgc
    15001 cagcagtttt tcgaccggat cgataacggt aacgttgtga ccacgcgctt ctaacacgcc
    15061 ggccataatg gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc
    15121 cgggcactgc cccaacaaac taatgccatg caggacatgt tttatttggg caaattcctg
    15181 atcgacgaaa gttttcaatt gcgccagcgg gaaccccggc tgggcggcgg cgagtcccgt
    15241 caaaagttcg gcaaaaatac gttcggcatc gctgatattg ggtaaagcat cctggccgct
    15301 aatggttttt tcaatcatcg ccaccaggtg gttggtgatt ttggcggggg cagagaggac
    15361 ggtggccacc tgcccctgcc tggcattgct ttccagaata tcggcaacac gcagaaaacg
    15421 ttctgcattt gccactgatg taccgccgaa cttcaacact cgcatggttg ttacctcgtt
    15481 acctttggtc gaaaaaaaag cccgcactgt caggtgcggg cttttttctg tgtttcctgt
    15541 acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg tggtggtaat ggtggtgcta
    15601 atgcgtttca tggatgttgt gtactctgta atttttatct gtctgtgcgc tatgcctata
    15661 ttggttaaag tatttagtga cctaagtcaa taaaatttta atttactcac ggcaggtaac
    15721 cagttcagaa gctgctatca gacactcttt ttttaatcca cacagagaca tattgcccgt
    15781 tgcagtcaga atgaaaagct gaaaaatact tactaaggcg ttttttattt ggtgatattt
    15841 ttttcaatat catgcagcaa acggtgcaac attgccgtgt ctcgttgctc taaaagcccc
    15901 aggcgttgtt gtaaccagtc gaccagtttt atgtcatctg ccactgccag agtcgtcagc
    15961 aatgccatga ctcgttcgcg taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt
    16021 ttcgccggtt gttgtattaa tgttgctaat tgatagcaat agaccatcac cgcctgcccc
    16081 agattgagcg aaggataatc cgccaccatc ggcacaccag taagaacgtc agccaacgct
    16141 aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc
    16201 catgaagatt tttcctctaa cagcggcacc agttctactg gcgtggcgta gtaatgatat
    16261 ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga catcgtgtaa cgattcaacc
    16321 agtgtcggga aaactttaat attatcaata atatcaccag atccatgtgc gacccagcgg
    16381 gtggctggct ccagatgtgc ctgactatcg acaatccgca gttcgctaaa acccatcgtt
    16441 ttcattgccc gcgccgctgc cccaatattt tctgctctgg cgggtgcgac cagaataatc
    16501 gttatacgca tattgccact cttcttgacc aaataaccgc gaacagggtg atcactgtca
    16561 acttattacg cggtgcgaat ttacaaattc ttaacgtaag tcgcagaaaa agccctttac
    16621 ttagcttaaa aaaggctaaa ctatttcctg actgtactaa cggttgaatt gttaaaaaat
    16681 gctacatatc cttctgttta cttaggataa ttttataaaa aataaatctc gacaattgga
    16741 ttcaccacgt ttattagttg tatgatgcaa ctagttggat tattaaaata atgtgacgaa
    16801 agctagcatt tagatacgat gatttcatca aactgttaac gtgctacaat tgaacttgat
    16861 atatgtcaac gaagcgtagt tttattgggt gtccggcccc tcttagcctg ttatgttgct
    16921 gttaaaatgg ttaggatgac agccgttttt gacactgtcg ggtcctgagg gaaagtaccc
    16981 acgaccaagc taatgatgtt gttgacgttg atggaaagtg catcaagaac gcaattacgt
    17041 actttagtca tgttacgccg atcatgttaa tttgcagcat gcatcaggca ggtcagggac
    17101 ttttgtactt cctgtttcga tttagttggc aatttaggta gcaaacatgc agaccccgca
    17161 cattcttatc gttgaagacg agttggtaac acgcaacacg ttgaaaagta ttttcgaagc
    17221 ggaaggctat gatgttttcg aagcgacaga tggcgcggaa atgcatcaga tcctctctga
    17281 atatgacatc aacctggtga tcatggatat caatctgccg ggtaagaacg gtcttctgtt
    17341 agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa
    17401 cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat gactacatca ccaaaccgtt
    17461 caacccgcgt gaactgacga ttcgtgcacg caacctgctg tcccgtacca tgaatctggg
    17521 tactgtcagc gaagaacgtc gtagcgttga aagctacaag ttcaatggtt gggaactgga
    17581 catcaacagc cgttcgttga tcggccctga tggcgagcag tacaagctgc cgcgcagcga
    17641 gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact
    17701 gctgaagaaa atgaccggcc gtgagctgaa accacacgac cgtactgtag acgtgacgat
    17761 ccgccgtatt cgtaaacatt tcgaatctac gccggatacg ccggaaatca tcgccaccat
    17821 ccacggtgaa ggttatcgct tctgtggtga tctggaagat taatcggctt taccaccgtc
    17881 aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa ccttatttcc agatacgtaa
    17941 ttgatcgtcc attgtaactt ctttactggc tttcattttc ggcagtgaaa acgcatacca
    18001 gtcgatatta cgggtcacaa acatcatgcc ggccagcgcc accaccagca cactggttcc
    18061 caacaacagc gcgctatcgg cagagttgag cagtccccac atcacaccat ccagcaacaa
    18121 cagcgcgagg gtaaacaaca tgctgttgcg ccagcctttc aataccgcct gcaaataaat
    18181 accgttcatt aacgccccaa tcagactggc gattatccat gccacggtaa aaccggtatg
    18241 ttcagaaagc gccagcaaga gcaaataaaa catcaccaat gaaagcccca ccagcaaata
    18301 ttgcattggg tgtaagcgtt gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt
    18361 cagcgcaatc agcagaatgg catacttagt cgcccggtca gttaattggt attgatcggc
    18421 tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc cagccggtat cattgcctga
    18481 agcaaaacgc tcaccgagat tatttgcaaa ccagctgctt tgccattgcg cctgaaaacc
    18541 tgactcgcta acttcccgtt tggctggcaa aaaatcacct aaaaaactgg gatgcggcca
    18601 gttgctggtt aaggtcattt cgctattacg cccggcaggc accacagaaa gatcgccggt
    18661 accgcttaaa ttcagggcca tattcagctt caggttctgc ttgcgccagt ccccttcggg
    18721 taaagggata tgcacgccct gcccgccttg ctctaacccg gtaccgggtt cgatggtcag
    18781 cgctgttccg ttaacttcag gcgctttcac cacaccaata ccacgcgcat ccccgacgct
    18841 aatcacaata aatggcttgc ctaaggtgat atttggtgcg tcgagttcgc taagacgcga
    18901 aacatcgaaa tcggctttta gcgttaaatc actgtgccag acctgaccgg tataaatccc
    18961 tatcttgcgt tcttcaacgt tctgattgcc atcaaccatc aatgactcag gtaaccaaaa
    19021 atggataaaa cttcgtttcc gctccacggt tttatcgtct tcctgcaccg tataaagctc
    19081 ggtcacagga atagcgatga gcggcccaac gagtttttgc ggcccgctgg tactttggcg
    19141 aatcgcatct tccacatcgc tacggtaatc ggcacgttcg acaatcacct gccgaatcag
    19201 cattatcgga atcaacaaca gcaatactgc accaaacagg gtggtcattt tccagaacag
    19261 gggggatttc aacatagtct tctcctttgc aatggcagca ggatacgaag gctatgtggg
    19321 aagaatttga agctatgtga agtgacggtg aagtcgaagc gaggccagca cgccaccttc
    19381 ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc
    19441 caaccccaga ccgctgcttt tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa
    19501 aatacgtgac agcgcgtagt caggaatacc actaccggta tccagcacct taagcgtgac
    19561 gtgttcctga tccacttcgg cgcttagcgt tatacgaccg ctctcggggg taaaatcgat
    19621 ggcgttatcc agtaaattcc ccagcgcctg atccagtaac gccggttcag cagcaacatt
    19681 aacctcggtg ggcgtaacat gtagagtgat gtttttttct gccaactgca cggtgcgcgc
    19741 ttcgctgacg cggcggaata atgccgccac atcaacagca gtcaaaacga cttcctgacg
    19801 attctccagt cttgcctggc gtagtaacgt ttccaccagt gcctgcattc gcgcattttg
    19861 cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc ggcggacctt cgcgtaaaat
    19921 ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc tcatgggtta acgcataaac
    19981 atactgctca atatagtttt tcccttccag cttcacgcgc atactttcca gcgcctgcgc
    20041 gagtttacgc agctcgctac taccgagatc gggaagagga acgggcttat tgtcagtgac
    20101 ggaatcagca tagcgagtga gccgggcaat ggagcggttg atccaccaaa ccatgcctgc
    20161 gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc cataaaattc gccgctcgct
    20221 gcgtttaatg accggagcca tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag
    20281 ccgcgagccg tccataatcg gcgcggcaac atacatcacc gaactttcgg gatcggcagg
    20341 attttgcaac gtgctgcgcg caccatactg accacgcaac gttagccaga cgtcattcca
    20401 gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg aacaatactt taccctgcga
    20461 atcggtcata tagacatgat attcattgcg cactttgtta atgccaccga tattggcgcg
    20521 aaacgggcga tgttgtagct gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc
    20581 agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt gcggtgtcga tcaacgtccc
    20641 ctccgttgct cttcgcacgc ccggtttaac ttctttgaca aaaatagcca gtacgaacca
    20701 ggccgccacc gccaccagta aaaaatagcc cagcagcaac cgcatgccga tacgcattac
    20761 aggcccctca ggctatatcc catgccgcga tgagtattaa tcggtgaaag atcgggattg
    20821 atggcgcgca gcttggcacg caacgtttta atgtgggtat caacggtgcg atcataggtg
    20881 tcctgcgcat cttcccacac gctatccatc agttgctggc gggaccagac gcggcctggc
    20941 gacttgagta acgtcttcag caataaaaac tcataccgcg tcagcgttaa tggcgtgtca
    21001 aaccagctga tctgcgccgc gggttcattc aattcaaaat gaccaatacg gatgacggga
    21061 gacggcgacg agaacttctt cacccgacgc agtaaggtgc gcaccctggc gcacacttcg
    21121 cggggtgaaa acggtttagc cacatagtcg tcagcgccaa tttccagccc aagcaggcga
    21181 tcgacctctt cacttcgggc cgtcaggaac agtacaggta acgccggatg gagcgccagt
    21241 aactggcggc acaattcaaa gccgctgata tcaggcaaac caacgtcgag aattatgacg
    21301 tcgggaacca gctggcgagc tttatccagc accggcaagc ctcgctcaaa gacctcgacg
    21361 gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc
    21421 actaaccaga ccgtttcccg ttgcataaat cgcctctgtt attgccgcca cggcatgaca
    21481 ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga
    21541 taagcgagcg cgttgcgttt agcatcataa aagcgcacga cctgtaacga cttaaagacc
    21601 agggacgtac gttttttgaa tactacctcg ccctgagctt tgccgttttt aatacgatcc
    21661 gacagttcaa tcggcccgac ttgctgacaa gaaatggccg catcggaggt atcttccgcc
    21721 aggcccaatc cccctttaat acccccggtt ttcgcccggc tcacataaca ggtgacattt
    21781 ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt gatccgggcc gatcatttta
    21841 aatacggtgt cgaccgaacc aatctcttct gcatgagcca atggccccag cattatcagg
    21901 cttaaagaca ggatcaaatg cttgtatttc atattgttac catttttatc tattacacga
    21961 gcaattagtt cgtcacggta aataacatat tgattaagaa caaaatctca atacttttat
    22021 ttccgtcaag ccctaaaaca tactctacta agaaaaaaac actgaatgct aaaacagcaa
    22081 aaaatgctat tatccaatta cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt
    22141 ttatggatca ggccggcatt attcgcgacc ttttaatctg gctggaaggt catctggatc
    22201 agcccctgtc gctcgacaat gtagcggcga aagcaggtta ttccaagtgg cacttacaga
    22261 gaatgtttaa agatgtcact ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt
    22321 cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat tctggacatc gcgctgcaat
    22381 accgcttcga ctctcaacag acatttaccc gcgcattcaa gaagcagttt gcccagactc
    22441 ctgcacttta ccgccgttct cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc
    22501 tgggtgaatt cactatgcca gagcacaaat ttgtcaccct ggaagatacg ccgctgattg
    22561 gtgttaccca gagctactcc tgttcgctgg agcaaatctc tgatttccgc catgaaatgc
    22621 gttatcagtt ctggcacgat tttctcggca acgcgccgac cattccgcca gtgctctacg
    22681 gcctgaatga aacgcgtccg agtcaggata aagacgacga acaagaagta ttctatacca
    22741 ccgcgttagc ccaggatcag gcagatggct atgtactgac ggggcatccg gtgatgctgc
    22801 agggcggcga atatgtgatg tttacctatg aaggtctggg aaccggcgtg caggagttta
    22861 tcctgacggt atacggaacg tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg
    22921 atattgagcg atactacccg gcagaagatg ccaaagcggg agatcgccca attaatctgc
    22981 gctgtgaact gctgattccg atccgtcgtt aacgctgcaa ctcatctaat gcaggggcgt
    23041 ccagatgtga gatgtcccct gcggtttcaa caacccagcc agacgccagc cacaggcttt
    23101 cctgataatc cacgcgcgaa atagaacagt tacgcagacg taagcggcgc tctgcccatg
    23161 ctggtaatcc gagaatcgta ctcaccagac atcccagtgc aataccatgg ctcaccagca
    23221 gcggtcggct tccctgcggt aagtcccggc aagattccag tgccgcattg acgcgatcgc
    23281 tgagctcctg cattgactcg ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct
    23341 gccgacgcca gttctcttct tcttcggtca gagaatcgat atgtcttttt tccagcacac
    23401 ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc acagccgcag gcctgggcga
    23461 tgatttccgc cgtacgccgg gtgcgtccta aatcgctact gatgatatgc gtaatgccaa
    23521 gctctttggc acgggttgcc acctgcatcg cctgttgctc acctttggcg gtcagcgggc
    23581 tgtcagactg gccctgaata cgtcgctcgg cgttccactg cgtttcaccg tggcggacta
    23641 ggtatacctg taacatgctt tttttccgta tactgctgta acgttaattt tctgagtaat
    23701 gctgattatg caccaagttg tctgtgcgac caccaatccc gctaaaattc aggccattct
    23761 gcaggcattt cacgagatct tcggcgaagg atcctgccat attgcatccg ttgccgtcga
    23821 gagcggtgta ccggaacagc cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg
    23881 ggtagcaaat gcccgccgtt tacttccaga ggctgatttt tgggtggcga ttgaagctgg
    23941 catcgatggt gacagcactt tcagttgggt ggtgattgaa aacaccagcc agcgcggcga
    24001 ggcgcgttcg gctactttgc cgttacctgc ggtgattctg cagaaagtgc gtgaaggtga
    24061 ggcgcttggt cccgtgatgt cgcgttatac cggtattgat gagattggtc gtaaggaagg
    24121 ggcgatcggc gtgtttaccg cctggaaact cactcgcgcc agcgtctatc accaggcggt
    24181 gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag gcattgtagg acggataagg
    24241 cgttcacgcc gcatccggca ttcggtgcac gatgcctgat gcgacgctgc cgcgtcttat
    24301 caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactgg cgcagctcca
    24361 cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgccc gcgccgagtt
    24421 cattttttaa ctcacgctgg ctcatttcgc cgcgcaacag ctcttcgaca atacgcacgc
    24481 gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag atttaacaac ggtaaatgga
    24541 gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc tggtgacgct
    24601 gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc cattgttagc
    24661 gggggaagca aaatgcctcc cccggttgta ctcgctaaag agtacgatag catatcataa
    24721 acgtgcggat cagtaacgac gtccccattc cgtggcgctc atcaacgtcg gtttatcccc
    24781 catgaaatag cggtagtaag cgtcataagc cagcacgttc ttcacataac cgcgcgtctc
    24841 ggagaacgga atactctcga caaatgccac agcgtcgata cgcccggcgc tgttgccaag
    24901 ccaggttcgc acccgccctg gtccggcgtt ataagctgct gaggagaaaa tacggttatt
    24961 gccaaactgc tgataaacat attgcagata actggtgcca atgttgatat tcgtttccgg
    25021 atccagcaat tgcccagggc tactataacc gggaatagag aacatcttca ccgtatgggt
    25081 cgctgtacca ggcataatct gcatcaggcc gctggcccct accggtgatt tcacttttgg
    25141 attccaggcg ctctcctgac gggcaatcgc catcgcatag ctttgcggga tctccttacc
    25201 gctggtgtag cgtttgaaaa gatcgttgta agccagcggg aatcgctctt ccagatgatc
    25261 ccatagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt tgttgaaagc
    25321 ataccgcgcc agttgagcct gctctgtttt tgacttgctc ttcaccagat tggcccactc
    25381 gctacgcgcg gtgttatcga gattccagta catcaactcg cgcacgcgcg ccatctccgg
    25441 cccctgagtc agggcgctgt caacattctg cggcgcttta tcaatcttca gctcatactc
    25501 ttctccgatg cgttgtgctg caaccatcgg gtagaaacca cgctgttgca tgagttgatg
    25561 caaaatctct ttcgcttcag cttcacgtcc gcgttccagc aataaatccg cctgccagta
    25621 acgccattca tctttctctt ttgcttccat cggcagacgc gccagccagg tattcaggcc
    25681 gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt tcaatcaacg acgtggattg
    25741 cgagcgcata atggcgtcat cgcgccattt cgcctgctcg tcggtgacat cgttgcccat
    25801 caaacgccag gcgacgatat cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc
    25861 ctgggcaagc gacgggatca tcagccgtgc attttcagca tcctgccgcg ccacactggc
    25921 aaacgccacc gctgccattt gacgggtaaa atcggtcgct ccggttgtac gcacgaaggt
    25981 cagtaccgta ttagggttgt tcgccagtga aatgattgcc gaggcgatag tctggtaatc
    26041 ggcaggcatc tgccctgcca gcactgttac caggccagtg ttacccgctt tcatcgccag
    26101 acggatacgc tctaaatacg ccagcggatc ttgtttacct gacgcacgcc agacgctaaa
    26161 taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt tcgccccttg
    26221 ccaggcttct tcactctgcc cggtgttcca tttcgcatag tagtaattac attgcgcttc
    26281 ggtagttccg ggcttttccg ggctaaaggc taacaagcca cgccagtctt cacgccgcgc
    26341 cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg
    26401 aacaaagtta gtgaccgtca ccgccggttg attcatcaga tcatcggtga tctggcggta
    26461 ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt cgaccacatc
    26521 catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa cggctacgct gctcatccag
    26581 tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag ccaacagccg
    26641 ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat acatcaatgc
    26701 agcgtcatgc cgcgtaatag atttatgcta accagtcatt gccgtttacg ccacgttacg
    26761 gacacttttt tacttttact gcgaaggtga tcgaccaact gggattaggc ggggaaaaag
    26821 gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa gtccaacgtg
    26881 gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt
    26941 ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac
    27001 ggcgcgggta agtctaccct gctgcgcatt atggcgggca ttgataaaga catcgaaggt
    27061 gaagcgcgtc cgcagccaga catcaagatt ggttacctgc cgcaggaacc gcagctgaac
    27121 ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt ctgaagtggt taacgccctg
    27181 aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt tgacaagctg
    27241 gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca taacctgaac
    27301 gtacagctgg agcgtgcggc ggatgcgctg cgtctgccag attgggacgc gaaaatcgct
    27361 aacctctccg gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgcttct ggaaaaacca
    27421 gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt ggcctggctg
    27481 gaacgcttcc tgcacgactt cgaaggcacc gttgtggcga ttactcacga ccgttacttc
    27541 ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg gtgaaggtat tccgtgggaa
    27601 ggcaactact cctcctggct ggagcagaaa gatcagcgcc tggcgcagga agcttcacaa
    27661 gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca gggaactaaa
    27721 ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg aagagctgaa cagcaccgaa
    27781 tatcagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg tctgggcgat
    27841 aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggcg atcgcctgct gattgatgac
    27901 ctgagcttct cgatcccgaa aggggcgatc gtcgggatca tcggtccgaa cggcgcgggt
    27961 aaatcgaccc tgttccgtat gatctccggt caggaacagc cggacagcgg caccatcact
    28021 ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc gtgactcaat ggataacagc
    28081 aaaaccgttt gggaagaagt ttccggcggg ctggatatta tgaagatcgg caacaccgag
    28141 atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca gggtaaacgc
    28201 gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt
    28261 ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga aaccctgcgc
    28321 gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc gcacgaccgt
    28381 tggttcctcg accgtatcgc cacgcacatc ctggactacc aggatgaagg taaagttgag
    28441 ttcttcgaag gtaactttac tgagtacgaa gagtacaaga aacgcacgct gggcgcagac
    28501 gcactggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt aaaatgccgg
    28561 atgcggcgtg aacgccttat ccggcctaca aaccaagaaa tttcaatacg ttgtgaatct
    28621 ccaggcctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa tgccggatac
    28681 ggcgtgaacg ccttttccgg cctacaacag ccttagtatc cgcaaattcg tagcgggtca
    28741 cccgtgctga ttcttttaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca
    28801 actcatcaaa aaacttattt tatacctcgc ctttgagccg tttcatcgcg gttatctctg
    28861 ctcccccatc atctcccgca ccaactccac gcagcgcagg aaacggctgt cataatcgtc
    28921 ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc atctccacca gcaagttctg
    28981 gaactctttg cgatccaccg aactgccgag gctgcgtaaa ccatccgcca cccacggcgt
    29041 gttgttctcc agcaggatca ccagatcgaa acggtattca tcaatcaacg cctgtacgaa
    29101 cggatgctca cgcccttcgt actttttgca gaacgcctgg gtagtgacaa aatcagtgtc
    29161 aataaacgcc actttattgg catatttcac tgcaaaatca atgtattgtg cgtggcccag
    29221 cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa
    29281 gacataatcg cgaccatatt cccacgcact ggtggtattg aagatattgg caagtttgtt
    29341 taccagggtg gatttaccgc tcgactcgcc accaaggatc gccacggtac gtacaaagaa
    29401 cggcttcact tccgtaggaa tatattccca gtagcggaac ggattttcgc ggatctgcgc
    29461 accgctgata ctcataaagg tacgtttcgg atcgaccagc accgtatcga tccccagatg
    29521 ttccatatac tgtggcgcat cggcttcttc cgaggtgtag atcaggtccg gctgaatccc
    29581 cttttcagcc ataaactttt tgatgccgtt gctccacaca tcccagccgt gcggatacgg
    29641 ctccatgccc tcttcgttga aagcatgaat gcgaatattt ttctgatatt taaaagtttg
    29701 taataaccag cgcagacgat ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa
    29761 caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata tgcagctcgt caacctggct
    29821 acaggcgcgc tggataaggt agatatgtcc ggtatgcagc gggtagaact taccgaatac
    29881 gacaccaatg gttttcttct gtcggggaaa ttcaagcccc aaaaaacggt gcaacgcctc
    29941 cagcttttgc gcgctggggc ttttaatttt ggcattcagt aactggctta aatacccttt
    30001 ggtcataccg ctggcatcag ctacctgctg tagcgtgcag ccctgttgct tgatggcagt
    30061 tttcaggtaa tcaaatgacg acatatctcc ctccgtatct ctcattataa gtcgtcgaac
    30121 acgctaagcg cgtcggagag ttttttaacg ccaaaaatct gcatcccttc cggcgctttt
    30181 ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac cgtgtttcgc cgcttctgaa
    30241 attcgttcct gaccgctggg caccgggcgg atctcccctg ccagcccgac ttcaccaaac
    30301 accaccagat cctgtggcag cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac
    30361 gctaaatcgg cactggtttc ggttaccttc acgccgccga ccacgttcac aaatacatcc
    30421 tgatcggcca tttgcagacc accgtgacgg tgcaagacag ccagcaggat tgccagacgg
    30481 ttttgttcca gccccactgc cacgcggcgc ggattcgcca tcatcgagtg atcgaccagc
    30541 gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca ccaccatcac ggagctgccg
    30601 gaggtcactt catctccgcg acttaagaaa attgccgaag ggttgctgac ttcacgcagc
    30661 ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg cgccgaagcg gtttttatgg
    30721 ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca gaagcaccga acagtcgata
    30781 cagtgttcca gcactttagg gccagccagc gagccatctt tggttacgtg cccaaccatg
    30841 acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat aagccgccgt ttcacgcacc
    30901 tgcgccacgc taccaggcga tgattgtaca tccgccatat gcatcacctg gatcgagtca
    30961 attaccatca gtttcggttg ctcttcttcg gcaatcaggc agatctgctc gatgctggtt
    31021 tccgacaaca tattgagatt gtcagtcggc aggccaagtc gatgagcgcg cattgccacc
    31081 tgttgcagcg actcttcgcc ggtgacatac agcgttttca tctgctgggc cagtttgcac
    31141 agcgtttgca gcaacagcgt ggatttcccc gcaccagggt taccgccaat cagaatggca
    31201 cttcctggca ccacgccgcc gcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa
    31261 cgcggcagct cttcaaggct gatatcggag agtttctgga cttttgccac cccggcgcta
    31321 ccggcatagc cgctgagacg ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc
    31381 tcggtgatgg tgttccaggc atgacaggca ctgcactgcc cctgccagcg cggataatcg
    31441 gccccgcatt cattacaaac aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt
    31501 aatgctgtgc cgcccgcagg atggcgggcg agcaattact tctgattcag gctgcctgag
    31561 aggatgcaga atacccccat caggtcagcg tgacggatgg tgacttccgc cttttcattc
    31621 acttttggct tggcatggta ggcaatcccc agccctgctg ctttgatcat cggcaggtca
    31681 ttagctccat cgccaatcgc cacggtctgc gccagcggga tttcatactc ctgcgcgagg
    31741 cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt cgccgatcac attgccggta
    31801 aatttaccgt ccatgatctc cagttcattg gctaccacgg cggtcaggcg cagcttgtcg
    31861 cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa tcgccacttt ccagcccagc
    31921 gtttccagct tgagcaccag ttgcgttaag cctggcatca gcggcagatt ttcacgcacc
    31981 tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca cacggctgcg caggctggcg
    32041 gtaaaatcga gttcgccgcg catcgcccgt tcggttactt ccgccaccat ctcgcccgtt
    32101 ccggccagtt tggcaatttc atcaatacat tcaatctgga tggcggtgga atccatgtcc
    32161 atcaccagca aacccggcgt gcgcaggtgc gggattttcc ccagcggggc gacatccagt
    32221 tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg aacctgccag acgaatcacc
    32281 tgataatctt ccacgcacca ggcggcaaca atcaccatcg ccgcacccag tttgctctgg
    32341 tattgggtca gacgctgttt atccagccca cgaccataca gcagccagcc gctacgacct
    32401 gcgtggtaat ccagtggcat cacttcatca ccacttaatg aaagaggcag acccggccat
    32461 aaagagacat cttcaggcag gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa
    32521 tcgttcgaag cagggaaaat aacgcatgag gctaccttgt atccattgct tctggcaaca
    32581 ttaagtctca aattttcaaa gggtggaaga tggctcgcac aaaactgaaa ttccggctgc
    32641 atcgggcagt gattgtcctg ttctgtcttg ccttgttagt ggcactgatg cagggggcgt
    32701 catggtttag tcaaaaccac cagcgacagc gtaatccaca gctggaagaa ctggcccgca
    32761 ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat gcgtaccgac tcaccagatg
    32821 aaaaacgcat tcaggcgatc ctcgatcagt taacggatga aagccgcatc ctcgacgcgg
    32881 gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg cgaaagcgtc gaagtgcgcg
    32941 accggctggc gctcgacggt aaaaaagcgg gcggctattt taaccagcag attgtcgaac
    33001 caattgccgg gaaaaacgga ccgctcggct atctgcgcct gacgctcgac acccatacgc
    33061 tcgccaccga agcccaacag gtggataaca ccactaacat tttacgcctg atgttgctgc
    33121 tttcactggc aatcggcgta gtgctgaccc gcacgctgct acagggtaaa cgtacccgct
    33181 ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg
    33241 agaaaaaaga gtgacccatt actacaagaa aggaaatcgt tatgtccaca ttacgcctgc
    33301 tcatctctga ctcttacgac ccgtggttta acctggcggt ggaggagtgt atttttcgcc
    33361 agatgcccgc cacgcagcgc gttctgtttc tctggcgcaa tgccgacacg gtagtgattg
    33421 gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg gatggaagaa gataacgtcc
    33481 gcctggcacg gcgcagtagc ggtggcggcg cggtgttcca cgatctcggc aatacctgct
    33541 ttacctttat ggctggcaag ccggagtacg ataaaaccat ctccacgtcg attgtgctca
    33601 atgcgctgaa cgcgcttggc gtcagcgccg aagcgtccgg gcgtaacgat ctggtggtga
    33661 aaaccgccga aggtgaccgc aaagtctcag gatcggccta tcgcgaaacc aaagatcgtg
    33721 gcttccacca cggcactttg ctgctcaatg ccgaccttag ccgcctggca aactatctca
    33781 atccggataa aaagaaactg gcggcgaaag gcattacctc agtgcgttcc cgcgtgacca
    33841 acctcaccga gctgctgccg gggatcaccc atgagcaggt ttgcgaggcc ataaccaagg
    33901 cctttttcgc ccattatggc gagcgtgtag aagcggaaat catctccccg gacaaaacgc
    33961 cagacttgcc aaacttcgcc gaaacctttg cccgtcagag tagctgggaa tggaacttcg
    34021 gtcaggctcc tgcattctcg catctgctgg atgaacgctt tagctggggc ggcgtggaac
    34081 tgcatttcga cgttgaaaaa ggccatatca cccgcgccca ggtgtttacc gacagcctca
    34141 accccgcgcc gctggaagcc ctcgccgggc gactgcaagg cggcctgtac cgcgcggata
    34201 tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc ggaccaggaa aaagagctac
    34261 gggagttgtc gacgtggata gcgggggcgg taaggtaact cgctgattaa aataccaaag
    34321 gaagggaata tgatcgatct ggaaaaccag gagcgggaga ttatcaatat aatgttaagc
    34381 cagcgtattt cctggctagc cgccgtgcgt atcaggcata aattatcgct tgctgaagta
    34441 agtaaaatgt taggtatctc gattaattct ctaaaataga tcgaaaagac ggaacggttt
    34501 agctcaaaca tcaaaagcaa aatggcagaa atttatggtt gtccaccaga gttgctgata
    34561 tgcccttcct ggatgacggc tgaacataaa tgaatagatt cgtgaataga tacttgaata
    34621 gattttattt tctatccata tgaaaaataa tagtaaatct ataaatcagt tgctattgat
    34681 gaatagatac taaaaagccg gagcagtctc ccactccggc ttcacacgac aatcgccttt
    34741 cagcgaaaca caattactct ttatcgccca gcagaacgga ttccagtgcg attttgatca
    34801 tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt
    34861 cagacacggt acagatggtc agggctttcg cgccaaattc tgcagcgacg ccgtagatac
    34921 cagccgcttc catttccacg ccgaggatgc cgtatttttc catcacgtcg aacatttcgc
    34981 cgtccggaga gtagaacagg tcagcggaga acaggttacc aacgcgagca tcaatgccca
    35041 gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa gtcagcgata gcggcaaagt
    35101 catgatcttt aaaacggatg cggttaactt tggaatcggt gcaggcaccc ataccgataa
    35161 cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca ggaacccacg cggataattt
    35221 tcttcacgcc gaaatcggtg atcagttctt tggtgtagat ggagcaggac gggataccca
    35281 taccgtgacc cattacggaa attttgcggc ctttataagt accggtgaag cccagcatac
    35341 cgcgaacgtt gttcacttca cgggcatctt caaggaaagt ttcagcaata tactttgcac
    35401 gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc gcccatttct gcattaatgt
    35461 gtggggtagc cattgtttta tccttatcta catgttttca ccctctccct gagggagagg
    35521 gccggggtga gggcatcaag ccgcacccat tatcagaaca tggctttgcc atattccata
    35581 tcagaagtac caaaatattt tgccagagtc tggccgatat ccgcgaaggt ttcacggtga
    35641 cccagtgagc ccggttttac tttcgggccg taaaccagta ccggaatgtg ttcacgcgtg
    35701 tgatcagtac cggtccaggt cggatcacaa ccgtggtcag cagtgaggat caggatgtcg
    35761 tcatcgcgca gcagagacat cagctccggc agacggcggt cgaacagttc cagacccgcc
    35821 gcataacctg cgacgtcgcg acggtggccc caggaagagt cgaagtcaac gaagttggtg
    35881 aagacgatgg tgttatcacc cgcttctttc atctctttga tggtggcgtc aaacagcgcg
    35941 tccaggccag ttgctttcac tttcttggtg ataccgcagt tggcgtagat gtctgcaatt
    36001 ttaccgacag aaaccacctg accgtgtttt tcatcaacca gtttctgcag cacggtcggt
    36061 gctggcggct caacagccag gtcgtgacgg ttaccggtac gctggaagtt accggctttg
    36121 tcgccaataa acggacgagc gataacacga ccgatgttgt agccgccgtt ggtcagctct
    36181 tcacgggcga tttcgcacag ttcgtagagt ttatccaggc caaaggtttc ttcgtggcag
    36241 gcaatctgga acacggagtc agcggaggta tagaaaatcg gcttgccggt tttcatgtgc
    36301 tcttcgccca gctgatccag aatgaccgta ccggaagagt ggcagttacc gagataacca
    36361 ggcaggttag cgcgttcaac cagtttatcc agcagctctt gcgggaagct gttttcgtga
    36421 tcggagaagt atccccactc aaacagaacc gggacaccgg cgatttccca gtgaccagac
    36481 ggggtatctt taccggatga catttcgtgc gcccatgcgt acgcgccgat aacttcagcg
    36541 ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt cgtgtgcttt cgccagcccc
    36601 agacgggtca gatttggcag attgagcggg cctttacgac cgttatcagc ttcgccttta
    36661 gcacaagctt ctgcgatatg acccagggtg tcagccccga cgtcaccaaa gcgttctgca
    36721 tcttctgtag cgccgatgcc gaatgagtcc agcaccataa taaatgcacg tttcatatgt
    36781 tctccgtact cagtgcttca aatttaaatg atcagatcag tataccgtta ttcgctgata
    36841 cggcgataga cagttggtgt gctttccggt gctttatcgg caagtttaat tgccgctttc
    36901 accgctttcg ccgcttcctg ccagctgttt tcgtctttcg cgtggataac cgccagcgga
    36961 cgctgaccgt ctacctggtc gcccagacgc gccatatcag taaagccgac gctgtaatca
    37021 atggtgtcag atgcctgacg acgaccgccg cccattgcaa ccactgccat ccccagcgcg
    37081 cgggtatcca tttcactgac aaaaccttcg gtatcagcat agactgcttt cgtcagcatc
    37141 gctgtcggca gatacttcgc gtagttctca acgaagtcgg ttgggccttt ttgtgccgct
    37201 accatacgac caaagacttc tgccgcttta ccgttgtcca gcactgcttg caatttcgcg
    37261 cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa gcatctccac gcacagcgcc
    37321 atcgtgacat caaacagacg cgggttacga tattcacccg tcaggaactg caccgcttca
    37381 cgaacttcaa ccgcgttacc tgcactggag gccagtacct gattcatatc ggtgagcagc
    37441 gcggtggtgc gcacgccagc gccgttagcc acgccaacaa tcgcttcggc aagggcttca
    37501 gagagttcgt aggtcggcat aaacgcgccg ctacccactt tcacgtccat caccagcgca
    37561 tccagacctt ccgccagttt cttcgccagg atcgaggcag tgatcagcgg gatggagtcc
    37621 acggttgcgg taatatcacg ggtcgcgtag aaacgtttat ccgccggagc cagtgagctg
    37681 gtctggccga taatcgctac gccgacgtct ttaataattt cgcggaaacg gttgtcatcc
    37741 gggaaaatgt cgaagccagg gatggattcc agtttgtcga gcgtaccgcc agtatgaccg
    37801 aggccgcgac cggagatcat cggaatatag ccgccgcagg ctgcgaccat cggccccaac
    37861 atcagcgaag tcacatcgcc gacgccgccg gtggagtgtt tatcaacaat cgggccattc
    37921 agatgcaggc ttttccagtc gagaacggtt cctgaatctc gcatcgccat ggtcagcgag
    37981 acacgctcag gcattgtcat atcgtggaag aaaatggtca tcgcgagggc ggcaatctgc
    38041 ccttcggaga tagtgttgtc gcgaataccg ttgataaaga aacgaatttc ttcatcgctt
    38101 agcgcatgac catcacgttt tttacgaata atttcttgtg cgagaaacaa ggtaacctcc
    38161 cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct acgccttatc cggcctacgg
    38221 gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag gcgtaaagca tcttacttag
    38281 tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt tcaacaggct tgccagcagg
    38341 ctggaagcac caaagcggta gtgacgcgca tctgcccagt cagcaccgaa cagttcatct
    38401 gcgatggcga gatatttctg cgcatcttcc gcagtacgca cgccgcccgc tggtttgaaa
    38461 ccaacggttt tttctacgcc catatcacgg atcacttcca tcatgatgcg cgcgctttcc
    38521 ggcgtcgcgt tcacagccac tttaccggta gaggttttga tgaagtccgc acccgctttg
    38581 atggagattt cagacgcttt acggatcagc gcttcgtctt tcagttcgcc ggtttcgatg
    38641 atcactttca gcagtacatt cgctgccgcg caagcctctt tacaggcttt caccaggtca
    38701 aaaccaacct gctcgttacc cgccatcagc gcgcggtacg ggaacacaac gtcaacttca
    38761 tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg cgatttcgat gtcatcgtta
    38821 ccgtgtggga agttggttac cgtagcgatt cggatttccg gggtgccctg ctctttcagc
    38881 gttttgcgag caatcgggat aaagcgagga tagatacaga tagcggcggt attgccgacc
    38941 ggagttttgg cctgatgaca cagagcaatt actttctcgt cggtgtcgtc gtcattcagg
    39001 gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg ctttcagatc agtcatttca
    39061 ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt tgttagtatt ctaacatcta
    39121 ctccgcaaca cacttcgata tacatcacaa ttaaggaaat ctacttacaa gtttgcatca
    39181 ctgtaatgcg atctggttca aataattcac tttcaaatga atgcgtcagt ggtggcaaac
    39241 gcatcaggat caaagtgaac atcacgaaac ttcttacaat ggcgcatcac cttcggcata
    39301 aaaggaaaag aagatgccca cttctcatga aaatgcactg caacaacgtt gccagcaaat
    39361 tgtcaccagc ccagtgctta gcccggagca gaagcgccat tttctggctc tggaagcaga
    39421 aaacaatctg ccttatccgc agcttcctgc cgaagcccgc cgcgcgctgg atgagggtgt
    39481 aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta
    39541 cgcccgtttt ctggcgaacg gttccgaatg gctggagctg gaaggcgcga aagatcttga
    39601 tgacgcactc tctctgctga ccattcttta ccaccacgta ccgtcggtca catcgatgcc
    39661 ggtctacctg gggcaactgg atgcgttgtt gcaaccgtat gttagaattc taacacaaga
    39721 cgagatcgat gttcgaataa aacgtttctg gcgttacctc gacagaaccc tgccagacgc
    39781 ctttatgcac gccaatatcg gcccgtctga ttcgcccatt acccgcgcga tcttacgtgc
    39841 agacgcagag ttgaagcagg tttcaccgaa cctgaccttt atctacgatc ctgaaatcac
    39901 ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt gaatgtagca aaccgcacat
    39961 cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg ggctacggga ttgtgagctg
    40021 ttacaactca ctgccgctgg cgggtggtgg cagcacgctg gtacgcctta acctgaaagc
    40081 cattgccgag cgcagcgaat cgctggatga cttctttacg cgcactctac cgcactactg
    40141 ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc ctctatcaac aatcacactt
    40201 ctttgagaat agcttcctgg tgaaagaagg gctgattaac cctgaacgtt ttgtgccaat
    40261 gtttggcatg tatgggctgg cggaagcggt taacttgctg tgtaagaaag aagggattgc
    40321 cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt tatcgcatca gcgcgcaact
    40381 ggcggagttt gtcgccaata cccccgtgaa atatggctgg caaaaacgcg ccatgttaca
    40441 cgcacagtcg gggatcagtt ccgatatcgg caccacgccg ggcgcgcgtt tgccgtatgg
    40501 cgatgagcca gatccgatca cccatctgca aactgtcgcc ccgcatcatg cttattatta
    40561 ttccggcatc agcgacattc tgacgctcga cgaaaccatc aaacgtaacc cgcaggcact
    40621 ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt gaatttaccg ccaatgtcag
    40681 cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt ttgtcggact tagaaaaata
    40741 tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc gaagaagccg cacgcaacac
    40801 tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa cagcagatgc gctttagtca
    40861 gtaagattat ccccttctcc tgcgttgacg ggccaggcag tcgtctggct ctgtttttgc
    40921 agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg gacgatggga cgttgcaatg
    40981 actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt gcagattgtt gacggcaaag
    41041 tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg cctgaagagg tgtccgcaac
    41101 atgccacgcc aatggcgcaa tccatgagcg tggacgaagt gcttagccat gtccgcaaag
    41161 cggtgctgtt tattgaaggg ataacggtaa gcggcggcga agccacgacc caactgccgt
    41221 ttgtggtggc gctgtttaca gccatcaaaa acgatccgca actgcgccac ctcacctgcc
    41281 tcgtggacag taacggtatg ttgagcgaaa ccggctggga aaaattactc ccggtgtgcg
    41341 acggcgcgat gctcgatctc aaagcgtggg ggagcgaatg tcatcagcaa ttaaccggac
    41401 gcgataatca gcagattaag cgcagcatct gtttgctggc agagcgcggt aagctggcgg
    41461 aactgcgttt gctggtaatt ccagaccagg tggattattt gcatcacatc gatgagctgg
    41521 cgacgtttat caagagactt ggcgatgttc cggttcgcct gaatgcgttt catgcccacg
    41581 gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc ggaagacgtt gagccgttgg
    41641 ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt tccggcgctc tatttgtgac
    41701 atgccggata cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgacaagcg
    41761 tcgcatcagg cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccact
    41821 aacgctaaac accgcatacg tattattaag caacacttcg gcaatctcat ccgccggttc
    41881 cgagcgcaac tcgcacagca cggcaaatac ccgggcggcc tgttccgggc ggttagcctg
    41941 cccctgaaaa ccattgagcg gcatatccgg cgcgtcggtt tccagcaaca acgatgccag
    42001 cggtaatttt gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc
    42061 tacgccgatt ttgtagccca gctgtacaaa ccgttcggcc tgttgcaggc tgccggaaaa
    42121 accgtgaacc acgccagtgc gcgataaatc gtggcgttta agatgcatcg ccagtttgtc
    42181 gtgtgtgcgc cgggaatgaa ggatcaccgg cagatcgtag cgtttcgcca gtttcagctg
    42241 ttcgtcgagt aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc
    42301 gatctccccc accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgatc
    42361 aagagacaca tcgctatgtt tttccaacat ccccggatgc aaccccaatg cggcataaag
    42421 cggttgatat ttttccgcta acgcctgcac gcgggcaaaa ttttccgcct ctgtcgccgg
    42481 aacaatgatc ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc
    42541 actaaacggc gggaaatcaa aatggcagtg ggtatcgata aaacggcaaa tcaagccaga
    42601 tcctcattgt taaatgtcgg gtcgttcgcc tgcggagcat cgctcacttc ggcaaccagc
    42661 gtatcgttgg cgactggcgc aggcggaata acgatcgatt ccggcgtcac cactggcacc
    42721 agatggcggg taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa
    42781 cgaccgcata aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc
    42841 gcgatactat gtaatggctt cggtggataa atttcgaata tccgcagctt gcccggtggt
    42901 ttctcaataa attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc
    42961 agcggctgca ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg
    43021 tagtacattt gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc
    43081 gcctctttaa ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc
    43141 gacactccgc tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag
    43201 ttttgtttgg ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata
    43261 taaaacgatt tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctga
    43321 cttgcggtgg cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc
    43381 aatggatcga aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg
    43441 ggctgattgc agatatacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga
    43501 tcgaaaggat taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca
    43561 cgctgtccgc cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac
    43621 aacggcgcaa tattaccaag cgttacaggt attcgctgcc ccacctctgc cttcctgttt
    43681 ttgttgtgaa agaatgacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag
    43741 tccgctggac tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg
    43801 gcccatgaac aaactcacca ggaacagaat aatcccgacg acaaagacaa ttttagctgc
    43861 gcctgcagcg gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac
    43921 cagaaatatg atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc
    43981 ttgatatgag cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc
    44041 ccgacaaagt tcgggaggac gaattacgac gaattactta gttttcagat catttttaac
    44101 gcttttcaca ccatctaccg ctttggcgat actttcagca cggtcgctct gtgcctggga
    44161 atcgacggta ccggagagct gaaccacgcc gtcggtggtt tcaactttca catgacggga
    44221 agggacgata tcgtccgcca gcagtttggc tttgatttca ctggtggtgg cggtgtcacc
    44281 cgcgtagccc ttcaccgagc cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga
    44341 ggtcacccct tcaacgcctt tcgccacttt cactgcctct tcggcctgag cctggctttc
    44401 aacgaaaccg ctcagggtca cgactttttg atcggttttg acagagatat cggtgctctt
    44461 gatgttgtca tgatccacca gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat
    44521 gaaattaccg actttattca tagagctatc gactttttgc cctgcgcttt cattggtagt
    44581 ctgtgcgttg ttttccgcgt aggcagagcc ggtcgcgacg gcagaggtca acattacagc
    44641 cagcagagtt ttcgaaatct tcagtcttgt catagtcatc gatttattcc tgtatgtttg
    44701 ctcgtaattt gagcctggca acacgaggtt gcattgctga atagggagag acttcaccct
    44761 ctacagaagt caatggtcgc catcacaaaa gcgatgggtg atgaataacg accattacag
    44821 cctctgaatc agttattaat atcggtagaa tgacaatcga cggctttaga tactgatatc
    44881 tacgcattga acggtattta acgccgtcag aaatgtcatt actttgttaa atatagttca
    44941 caattttgaa accgctcggg atatcagcga aaacataagc aaaagtgaat gttttaagaa
    45001 cattccgtaa gcggctaata aggaagggaa attgacaggg cgcagcggct accgcgccct
    45061 ggaggcaaga attaatgctc gcgggtctgg tggaactgaa cgtcgggata acgttcctgc
    45121 gccaggcgca ggttgaccat gctggtagcg atgtaagcga ggttatcgcc gccatcaagc
    45181 gccagttggc tttcgttctt acgcttgaac tcttcgaatt tcttcgcgtc cgcacattct
    45241 acccagcggg cagtggcaac gttgactgat tcatatactg cttcaacgtt gtattcgctc
    45301 ttcaggcgcg ataccaccac atcaaactgc agcacaccaa cagcaccaac gatcaaatcg
    45361 ttgttggaga tcggacggaa cacctgcacc gcgccctctt cggaaagctg taccagccct
    45421 ttgagcagct gtttttgctt cagcggatct ttcaggcgga tacgacggaa cagttctggt
    45481 gcgaagttcg gaataccggt gaacttcatc atctcaccct gggtaaaggt gtcgccgatc
    45541 tgaatagtgc cgtggttgtg caggccgagg atatcgcccg gatacgcttc ttcaacgtgc
    45601 gaacggtcgc ccgccataaa ggtcagcgcg tcggagataa ccacatcttt cgcagtacgc
    45661 acctggcgca gcttcatgcc tttttcatat ttaccggata ctacgcgcat aaacgccacg
    45721 cggtcgcggt gtttcgggtc catgttggcc tgaattttaa atacgaagcc ggtaaatttg
    45781 tcttcgctcg cctctacggt acgggtatca gtctgacgcg gcatcggcgc aggagcccac
    45841 tccaccaggc catccagcat atgatcaaca ccgaagttac ccagcgcagt accgaagaat
    45901 accggagtga tttcgcccgc aaggaacagt tctttgtcga actcgttaga cgcgcctttc
    45961 accagttcca gttcgtcacg tagctgctgt gccagatctt caccaaccgc agcgtcgaga
    46021 tccgggttat tcagcccttt aacaatacgg acttcctgaa tcgtgtggcc tttaccgctc
    46081 tgatagagat aggtttcgtc tttataaagg tggtaaacgc ctttaaacag cttgccgcag
    46141 ccaatcggcc aggtgatcgg tgcgcagccg attttcagct cgttctcaac ttcatcgagc
    46201 agttccatcg ggtcgcggat atcacggtca agtttgttca taaaggtgag gatcggcgtg
    46261 tcgcgcagac gggtaacttc catcagctta cgggtacgat cttcaacacc ttttgcagcg
    46321 tcgataacca tcaggcagca gtccaccgcc gtcagggtac gataggtatc ttccgagaag
    46381 tcttcgtgcc ccggggtgtc gagcaggtta accaggcaat cgtgatacgg aaactgcatc
    46441 acagacgtag taatggagat cccacgctgc ttttccatct ccatccagtc cgacttagcg
    46501 tgctggttgg aaccacggcc ttttactgta ccggcggtct gaatggcctg tccgaacagc
    46561 agcaccttct cggtgatggt agtcttaccg gcatccgggt gagaaataat ggcaaaagtg
    46621 cggcgcttcg ccacctcttg caaataagga gacaacgtca taattctttc ttcttgagta
    46681 agcggcatcg cgccgcgctt gttgaaaacg aaaaattgcg gctattttac ccatcaacgg
    46741 gggggaggca atcagtgttt acacaggagc tgctccagtt cgtgcaacga agaaacggtc
    46801 caggtgggcg cgatgccttc tggttgctcg cgatggtgcg cattcagcca gcaggtcgca
    46861 agcccggcgt tgatgccacc gagaatatcg gactcggcag tgtcgccaac catcagcacg
    46921 cgtgaacggt caggattgcc cgcctgttcc agcgcataat cgaaaatttt cttattcggt
    46981 ttggcaacgc caacttcttc ggaaatcacc agcaaatcga agtaatcacg caggcccgtg
    47041 cgttccagac gcacttgctg caaggcgcta aagccgttgg tgatgatgcc gattttggcg
    47101 ttgccacgaa tggcgttaag cagagaaacc gcgcccggca gcggcgtgca gatttccgcc
    47161 atcgcattaa taaaggcatc gttaagcaag cctgcttcaa ctttcagccg ttcggcccag
    47221 ctctcaaaac gcccgtgctg aagctgtaat gaagtgatcg cgccgttttg ataatccacc
    47281 cacagtggct tgttaacggc ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg
    47341 taatcaagaa acatccgctg caggccggtg aatgagtcaa aggtaaacag cgtttcatcg
    47401 gcatcaaaga aaatccagtc ccacttcatt attccacctt gtcttacata ctgattggca
    47461 acgccatgat gatggcgtct tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg
    47521 tcgcctcgtt aaagcctaaa ctttcgtaca gggcaatggc ggcagcattt gaggcacgga
    47581 cttccagcca tagtgtcgcc acgccgcgtt tttccagttc gtcgatcaga tgttccagca
    47641 gcacccttcc caatccctga cgctgatagt caggatcgac cgcaatattg aacaatgtag
    47701 cttcatccag caccacttgc gtaatcgcaa acgccgccat tttgccgttt tgcgttaact
    47761 gaaagttgag ataacgctcg ccctggttgc tggcaaaggt tttttcactc cacggaaagg
    47821 cgtgggcgcg ttgttcaatg tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa
    47881 tcgtgttcat atgtgcaaat ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt
    47941 aattcggtga gcgccggtga tgccacctga gcgccttcca gtgatagcgg ttcgtcagta
    48001 cccaaccgcc agctgttgca gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc
    48061 agttgcagca cttgatcggg gctgacggtt aatgcgcgca gaacatcgct cactaaagga
    48121 tcggtcaggg cgggaagatc gtttgccacc atcaccagac ggacgtgtgc cgggatcgca
    48181 atggcaatct ccccctgcaa cgcgccaggg cgacgcagcg accactgggt aatgcccagt
    48241 tgctgtaact gccagtctcg tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc
    48301 aaatatagca aattcgtcga taccgcgcca acatatggct ataatcgccg ccagtatcaa
    48361 ttgaggagca ttccatgtct gcatttaccc cggcaagtga agtcttgctg cgtcacagtg
    48421 atgatttcga acaaagccgt attctgtttg ccggagactt acaggatgac ctgcccgcgc
    48481 gtttagatac cgcggccagc cgtgctcata cccagcaatt ccaccactgg caggtattaa
    48541 gccgccagat gggggataac gcccgtttca gtctggtcgc cacggcggat gacgtcgcag
    48601 attgcgatac gctgatttac tactggccga agaacaaacc ggaagcccag ttccagttga
    48661 tgaatttact ttctctgctg ccggtgggga cggatatttt tgtcgttggc gagaaccgca
    48721 gcggcgtgcg cagcgccgag cagatgctgg cagattatgc gccgttgaat aaagtcgaca
    48781 gcgctcgtcg ctgtggcctc tattttggtc gtctggaaaa acagccggta tttgatgccg
    48841 ataaattctg gggcgaatac agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt
    48901 ttagccgcga cggtctggat gtcggtagcc agttgctgct ctcgacgtta actccgcaca
    48961 cgaaaggtaa agtgctggat gtcggctgtg gcgcgggggt gctttcagtt gcctttgcgc
    49021 gccattcgcc gaaaattcgt ctcaccttgt gcgatgtctc tgcgccagcg gtagaagcca
    49081 gccgcgcaac acttgcggcc aactgtgttg aaggtgaagt ctttgccagc aacgtctttt
    49141 ccgaggtgaa aggtcgtttt gatatgatca tctccaaccc gccgttccac gatgggatgc
    49201 aaaccagcct ggatgcggcg caaacgctga ttcgcggcgc ggtgcgtcat cttaatagcg
    49261 gcggcgagct gcgaattgta gcgaacgcct tcctgcctta cccggacgtg ctggatgaga
    49321 catttggctt ccatgaagtg attgcgcaaa ccgggcgttt caaggtgtat cgcgccatta
    49381 tgacccgcca ggcgaagaaa ggttaattat ctcgccgaat accgtgtcgg atgcggcgtg
    49441 aacgccttat ccgacctaca aaacctgcac gttagccctt cgtaggccag ataagacgcg
    49501 ccagcgtcgc atctggcatc tgcccccgca tgcctaccaa acctgcacgc tagtttcctg
    49561 atggacattt ttccagcaat tacacctccg gcgataatta actattgacg aaaagctgaa
    49621 aaccactaga atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg tatgcgaagg
    49681 tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt ccttacggac gtgggggttc
    49741 aagtcccccc cctcgcacca attatcttta cttcctttct tgtttcttcc ttgatttttc
    49801 atccctgatt caaattcatt atcatcagca tgatggcagc aataagcgcc aggcacgatg
    49861 gtagcaaaac gtaatgccgt aactgtttgt gatacagcat caccgacgtc aatactaatc
    49921 ccatcagaat gacaatccgc cactgatccg gtgataaatc cgcatacatc agccctgcaa
    49981 tcagcaatcc tggcagcaac actccccagc cactgcccag cgtacgttgc aacatggttt
    50041 catctctttc attgataatg ataaccaata tcatatgata atttttatca tttgcaagcc
    50101 agagaaatcc cttgctatcg ggtaaaccta tcgctaggat tagcaatcat tatcatttag
    50161 attgctatcc cgattatggc ctatcgttcc gcaccgctct atgaagatgt tatctggcga
    50221 acgcatctcc agccacagga tgctgggctt gcacaagccg tacgtgcgac gatcgccgaa
    50281 catcgtgaac atttgctgga gtttatccgc ctggatgaac ccgctccgct taacgccatg
    50341 acgctggcgc aatggtcatc accgaatgcg ctaagttctc tactcgcggt ctattccgat
    50401 catatctatc gcaaccaacc aacgatgatc cgcgagaaca aaccgctgat ctcactatgg
    50461 gcacaatggt atatcggcct gatggtgcca ccattaatgc tggcgctact gacgcaggaa
    50521 aaggcattag atgtgtcgcc ggaacatttc catgctgagt ttcacgaaac cggccgtgcc
    50581 gcctgtttct gggtcgatgt gtgtgaagat aaaaacgcaa caccacattc gccgcagcag
    50641 cgaatggaaa ggttaatcag ccaggcgctg gttccggttg tgcaggcact agaagcgacc
    50701 ggagagatca acggcaaact tatctggagt aataccggtt atttgatcaa ctggtatctt
    50761 actgagatga agcaactgct cggcgaggcg acggttgaat cgctgcgcca tgccctcttt
    50821 tttgagaaaa cgcttactac cggtgaagat aatccactct ggcgtaccgt ggtgctacgc
    50881 gacggcctgc tggtgcgccg gacctgctgc cagcgttatc gcttaccgga tgtgcagcaa
    50941 tgtggcgatt gtacgctgaa atgaaagcac tgccggggaa gtaaacccgg catcatgcag
    51001 attaataggg atgcaacaca ttacttgctt cgcaatgccg catacataac agaatatctg
    51061 cggcctccaa caaccctgca tcagaactga cgcccagttt cgacatcaca ttaaatttat
    51121 gtgcacggat cgttttgata ttgcgtttaa gctgctcggc aatttgtgtc attgagtagc
    51181 cacgcgacat aaagcgcaat atttctctct ccgtcgggct tagcgtccgg ctttggttaa
    51241 tgtaccactg attgttcagt cggtcggtcg cctgacgtac accatttaat gacaaaaaga
    51301 gttcctgatg aaaaatctcc agcgttgacg ctttacttaa tacaccgtcc agcggtgatg
    51361 gcgacaatga accaatcagc cgagcttcaa tatcatcatc cgcaataact aaacgccggg
    51421 tacgcggaaa cttaatcgcc agttcagtca ggcaagataa tccctctctg cgctcacttc
    51481 tcatggcaga aaaagaaaaa atgaccgccg caaatgagat ccgcgacatg gcatcctgaa
    51541 atgactcctg actggtatag gtatggagct tataagggtt acccgcaaac tttctgaata
    51601 aactctcaat accgatactg ctcatgacgc atttttctat gagtgcgaca tttctcttct
    51661 taattcggct gtgttccatt ctgcggcatc tccataaaca gaaggcaagt taagttctct
    51721 tgcaccctgc gcgctattta tccatgcata catttcagcg ttgctgtgaa ttgccagtcg
    51781 acgcatcgca ctgttttttt gggcgctgat agttttatta cttttcttaa gcaacgaagc
    51841 tatctggttg atgccccatc ctttacctaa tagccgcagt accttgcgct ctgaaagagt
    51901 gagaatgacg gagcgatagc taaaatcatg agtttcattc attgccggag aggtcagcat
    51961 ggcgctgata cgctctgcgt gcgtattgcc ggaacgtacg gtcttgacca gattctcaat
    52021 gggttcaaca tcagacaata acgtcgccgt agggcacatg agcaattcta ctgcctggga
    52081 ataccaggag cgtgacacca tgaaaaccca gtgaatttcc cgatattgtg agattaaaga
    52141 ataataatgt tcacaaacag aacgaggatc ttcactttca ccggctaaat cagcaatgac
    52201 taatccggaa cgacgtaatt gtaataaggt aaggtcctcc gcagaggcgc tggatattat
    52261 ttcatattca gggaagtgag tcctcatgac ctcttttaac cctgcttgca taacaggtat
    52321 tttactgata acaattccat ttttgcagca tcctggcaac atatttcctc cacatccact
    52381 gtctaaaaat catgatggtt tattcaggat attcattcct taaatgtttc cagcaaatta
    52441 atagattaag cgaataaatt atccttagca ttcactttca tatcagcata ctcctgctgc
    52501 acaacaactc cactgcatgt gtcagaaggt tagcatcaaa tgataacctg gtgaaatagc
    52561 ctggagacat ttaaaatatc tacattaagt tgtta
//
LOCUS       DAPHDX010000011        51129 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPHDX010000011 DAPHDX010000000
VERSION     DAPHDX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.011"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(192..1091)
                     /gene="pgrR"
                     /locus_tag="RH058_000656"
     CDS             complement(192..1091)
                     /gene="pgrR"
                     /locus_tag="RH058_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV3136292.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1229..2149
                     /locus_tag="RH058_000657"
     CDS             1229..2149
                     /locus_tag="RH058_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3136293.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2149..3081
                     /locus_tag="RH058_000658"
     CDS             2149..3081
                     /locus_tag="RH058_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3136294.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKSHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            3099..3737
                     /locus_tag="RH058_000659"
     CDS             3099..3737
                     /locus_tag="RH058_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3136295.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            4028..4756
                     /gene="mpaA"
                     /locus_tag="RH058_000660"
     CDS             4028..4756
                     /gene="mpaA"
                     /locus_tag="RH058_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV3136296.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLSQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4731..5696)
                     /gene="ycjG"
                     /locus_tag="RH058_000661"
     CDS             complement(4731..5696)
                     /gene="ycjG"
                     /locus_tag="RH058_000661"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV3136297.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5815..6321
                     /gene="tpx"
                     /locus_tag="RH058_000662"
     CDS             5815..6321
                     /gene="tpx"
                     /locus_tag="RH058_000662"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV3136298.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6365..7906)
                     /gene="tyrR"
                     /locus_tag="RH058_000663"
     CDS             complement(6365..7906)
                     /gene="tyrR"
                     /locus_tag="RH058_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV3136299.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(8054..9115)
                     /locus_tag="RH058_000664"
     CDS             complement(8054..9115)
                     /locus_tag="RH058_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV3136300.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9112..10509)
                     /locus_tag="RH058_000665"
     CDS             complement(9112..10509)
                     /locus_tag="RH058_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV3136301.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10664..11662
                     /locus_tag="RH058_000666"
     CDS             10664..11662
                     /locus_tag="RH058_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3136302.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVIRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11773..12678)
                     /gene="ompG"
                     /locus_tag="RH058_000667"
     CDS             complement(11773..12678)
                     /gene="ompG"
                     /locus_tag="RH058_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV3136303.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12723..13805)
                     /locus_tag="RH058_000668"
     CDS             complement(12723..13805)
                     /locus_tag="RH058_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3136304.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13819..14478)
                     /gene="pgmB"
                     /locus_tag="RH058_000669"
     CDS             complement(13819..14478)
                     /gene="pgmB"
                     /locus_tag="RH058_000669"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV3136305.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(14475..16742)
                     /gene="ycjT"
                     /locus_tag="RH058_000670"
     CDS             complement(14475..16742)
                     /gene="ycjT"
                     /locus_tag="RH058_000670"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV3136306.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELMNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16739..17794)
                     /locus_tag="RH058_000671"
     CDS             complement(16739..17794)
                     /locus_tag="RH058_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3136307.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17804..18592)
                     /locus_tag="RH058_000672"
     CDS             complement(17804..18592)
                     /locus_tag="RH058_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV3136308.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(18610..19662)
                     /locus_tag="RH058_000673"
     CDS             complement(18610..19662)
                     /locus_tag="RH058_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV3136309.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19693..20535)
                     /locus_tag="RH058_000674"
     CDS             complement(19693..20535)
                     /locus_tag="RH058_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV3136310.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20522..21403)
                     /locus_tag="RH058_000675"
     CDS             complement(20522..21403)
                     /locus_tag="RH058_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV3136311.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21424..22716)
                     /locus_tag="RH058_000676"
     CDS             complement(21424..22716)
                     /locus_tag="RH058_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3136312.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(22730..24409)
                     /gene="ycjM"
                     /locus_tag="RH058_000677"
     CDS             complement(22730..24409)
                     /gene="ycjM"
                     /locus_tag="RH058_000677"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV3136313.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(24621..24935)
                     /gene="pspE"
                     /locus_tag="RH058_000678"
     CDS             complement(24621..24935)
                     /gene="pspE"
                     /locus_tag="RH058_000678"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV3136314.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(25010..25231)
                     /gene="pspD"
                     /locus_tag="RH058_000679"
     CDS             complement(25010..25231)
                     /gene="pspD"
                     /locus_tag="RH058_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV3136315.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25240..25599)
                     /gene="pspC"
                     /locus_tag="RH058_000680"
     CDS             complement(25240..25599)
                     /gene="pspC"
                     /locus_tag="RH058_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV3136316.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25599..25823)
                     /gene="pspB"
                     /locus_tag="RH058_000681"
     CDS             complement(25599..25823)
                     /gene="pspB"
                     /locus_tag="RH058_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV3136317.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25877..26545)
                     /gene="pspA"
                     /locus_tag="RH058_000682"
     CDS             complement(25877..26545)
                     /gene="pspA"
                     /locus_tag="RH058_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV3136318.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26712..27689
                     /gene="pspF"
                     /locus_tag="RH058_000683"
     CDS             26712..27689
                     /gene="pspF"
                     /locus_tag="RH058_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV3136319.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27809..29074)
                     /gene="puuE"
                     /locus_tag="RH058_000684"
     CDS             complement(27809..29074)
                     /gene="puuE"
                     /locus_tag="RH058_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV3136320.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(29112..30392)
                     /gene="puuB"
                     /locus_tag="RH058_000685"
     CDS             complement(29112..30392)
                     /gene="puuB"
                     /locus_tag="RH058_000685"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV3136321.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30394..31881)
                     /gene="puuC"
                     /locus_tag="RH058_000686"
     CDS             complement(30394..31881)
                     /gene="puuC"
                     /locus_tag="RH058_000686"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV3136322.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(32156..32713)
                     /gene="puuR"
                     /locus_tag="RH058_000687"
     CDS             complement(32156..32713)
                     /gene="puuR"
                     /locus_tag="RH058_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV3136323.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32740..33504)
                     /gene="puuD"
                     /locus_tag="RH058_000688"
     CDS             complement(32740..33504)
                     /gene="puuD"
                     /locus_tag="RH058_000688"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV3136324.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33716..35134
                     /gene="puuA"
                     /locus_tag="RH058_000689"
     CDS             33716..35134
                     /gene="puuA"
                     /locus_tag="RH058_000689"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV3136325.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35283..35447
                     /locus_tag="RH058_000690"
     CDS             35283..35447
                     /locus_tag="RH058_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136326.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            35437..36822
                     /gene="puuP"
                     /locus_tag="RH058_000691"
     CDS             35437..36822
                     /gene="puuP"
                     /locus_tag="RH058_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV3136327.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            36956..37201
                     /locus_tag="RH058_000692"
     CDS             36956..37201
                     /locus_tag="RH058_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV3136328.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37514..39157
                     /gene="sapA"
                     /locus_tag="RH058_000693"
     CDS             37514..39157
                     /gene="sapA"
                     /locus_tag="RH058_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV3136329.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            39154..40119
                     /gene="sapB"
                     /locus_tag="RH058_000694"
     CDS             39154..40119
                     /gene="sapB"
                     /locus_tag="RH058_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV3136330.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            40106..40996
                     /gene="sapC"
                     /locus_tag="RH058_000695"
     CDS             40106..40996
                     /gene="sapC"
                     /locus_tag="RH058_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV3136331.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40996..41988
                     /gene="sapD"
                     /locus_tag="RH058_000696"
     CDS             40996..41988
                     /gene="sapD"
                     /locus_tag="RH058_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV3136332.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41990..42796
                     /gene="sapF"
                     /locus_tag="RH058_000697"
     CDS             41990..42796
                     /gene="sapF"
                     /locus_tag="RH058_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV3136333.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            42864..43217
                     /locus_tag="RH058_000698"
     CDS             42864..43217
                     /locus_tag="RH058_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDV3136334.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            43587..44375
                     /gene="fabI"
                     /locus_tag="RH058_000699"
     CDS             43587..44375
                     /gene="fabI"
                     /locus_tag="RH058_000699"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV3136335.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            44520..45647
                     /locus_tag="RH058_000700"
     CDS             44520..45647
                     /locus_tag="RH058_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV3136336.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            45715..47649
                     /gene="rnb"
                     /locus_tag="RH058_000701"
     CDS             45715..47649
                     /gene="rnb"
                     /locus_tag="RH058_000701"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV3136337.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(47727..47831)
                     /locus_tag="RH058_000702"
     CDS             complement(47727..47831)
                     /locus_tag="RH058_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136338.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            47885..49870
                     /gene="pdeR"
                     /locus_tag="RH058_000703"
     CDS             47885..49870
                     /gene="pdeR"
                     /locus_tag="RH058_000703"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV3136339.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            50017..50190
                     /locus_tag="RH058_000704"
     CDS             50017..50190
                     /locus_tag="RH058_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136340.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            50280..51029
                     /locus_tag="RH058_000705"
     CDS             50280..51029
                     /locus_tag="RH058_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3136341.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVYF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT        12227 a        13483 c        12783 g        12636 t
ORIGIN      
        1 atcgacgcca aaatgcggca aagcacatag aacaccgaac actacgttca ggcttcgcac
       61 gttcaaacca gttttcggta tcaaaatcag catctgtcgc cattgtacga agaataaaaa
      121 ataaccaaaa cgttgaaagg aactgagaat acgttaagag agaaattaaa ttaatgtatc
      181 agcaagtagt gctaaacagc acctttatac ttcagcctat ctataaataa tgagaatgcc
      241 gagccagcat gtcgacggtg tggatagtac aggtgataac cgggtaaatc tggtgtgaat
      301 ttatccaaaa cacgtatcag ttttttttct ttaatagccc gctcaacctg atcataaggt
      361 agatacgcca atccatgccc atcaattgca gcatcaatga tcaggtctat cgtattcagt
      421 aaaagctgac cttccatgcg aacacgaact tcacgtccac cgcgtattaa tctccagcga
      481 tttgctgtac ccgatgtggg aagatacaaa ttaattgcct gatgatctat taattgtgac
      541 actgacgttg gaacacttcg gcgagaaaaa taatccggtg agccaacaat agccattggt
      601 atatctggcc cgattcgaat ggcgatcata tctttatcca tctccccacc cagacggacg
      661 cctgcatcaa aacgttcaga aacgacatcg gtcaaaccat aatcaatggt gagctgaata
      721 tcaatttcag gatgcgattt caggaatgtg cgcattgctg gtaacaatat cgtttttgct
      781 gcatgttcta cagtagtaat acgtattgtc ccggatgggc ggttctgcag ctcgctcagg
      841 gatgccaggg ctgaatctat gtcatgcaac atagggccaa gaacagacaa aagatgctcg
      901 cccgcttcag ttggaacaac gctgcgcgtg gttcgcgtca gaagccgcaa tcccaatcgt
      961 tcttctatac gacgcactat ctggcttaaa gctgactgcg ccatgctcag gcgggctgct
     1021 gcacgagtga agctacgctc ctctgcaacg acgacaaacg ccatcagatc agcgatttct
     1081 tcacgcttca ttcagtatct ccatgcaatc tattaatcat ttaaatgata ggtctaatca
     1141 tgattttggg tctaatcaac ttaatgatca atatctatag tgatatcacc aaccgttaaa
     1201 acgaaccgac aactccatga ggaacggaat gatgaataat aaagtcagct tcactaacag
     1261 caataatcca accatctctt tgtctgcagt gatctatttc cctccaaaat ttgatgaaac
     1321 ccgtcagtat caggcaattg tggtatctca cccaggtggg ggcgttaaag aacaaacggc
     1381 cggaacctat gccaaaaaac tggcggaaaa gggatttgtc actattgctt atgacgcatc
     1441 ttatcaaggt gaaagtggcg gcgagccgcg tcagttagaa aacccataca ttcgtactga
     1501 agacatcagt gccgtgattg attacctgac aacgctttct tatgtcgaca atacccgaat
     1561 tggtgcgatg ggaatttgtg ccggtgcagg atatacagcc aatgctgcaa ttcaggatcg
     1621 ccgcatcaag gccatcggta ccgttagtgc ggtcaatatt ggttcgatgt tccgtaatgg
     1681 ttgggaaaac aatgtgaaat ctattgatgc cctgccatac gttgaagcgg gttcaaacgc
     1741 ccgaaccagc gatattagca gcggagaata tgccgttatg ccactggcac caatgaagga
     1801 gtccgatgcg ccgaacgaag aactgcgtca ggcctgggag tattaccaca cccctcgcgc
     1861 gcagtatcca acagcaccgg gttacgctac tttgcgcagc cttaaccaga ttattaccta
     1921 tgatgcttac catatggcgg aagtgtacct gactcagccg atgcagattg tggcgggcag
     1981 ccaggcaggg agcaaatgga tgagtgacga cctgtacgat cgggcatcaa gccaggataa
     2041 acgctatcac attgttgaag gcgcaaacca tatggatctt tatgacggca aagcctatgt
     2101 ggctgaagca atttcagtat tagctccgtt ctttgaggaa actctgtaat gaaacatatt
     2161 caaatccgca atagtgatat ggcctggcat attgctgcca atattcagtt tccaccaaat
     2221 tttgatgagt caaaacagta tccggcgatt atcagcgtcc atccttttgg cagttgtaag
     2281 gagcagacgt ctggcaatat ctatggcaaa gcattggctg aaaaaggcta tctcgttctc
     2341 gcttatgacg ccagtttcca gggagaatct ggtggggagc ctcgctggat agaagatccg
     2401 acacagcgcg tagaggatat tagccgtgtt atcgattatg cagtaactct gccatatgtt
     2461 aatgctgaac gtattggcgt gctgggagtg tgtgggggtg gtggctatgc cattaatgcc
     2521 gcgctgacag aaaaacgaat taaagcagta gtgagtatta cgggtgttaa tatcgggcgt
     2581 ctttttcggg aaggtttcag caattacgat ccgattggcg cattgaatgc aatggcgtcg
     2641 caacgaacca aagaggctcg tggtggagaa ttacagatca acgaattact tcccgccagc
     2701 ctggatgcag caaaatccca tggactgacc gaacgcgatg tttatgaagc aactgactac
     2761 tataaaacac cacgcgggca acaaccggga ggtgcgacaa aaatgttgtt ctcacatgca
     2821 cagaaaacgc tggcctggga tgccttcgca tttactgaag tgttactgac tcagccggtg
     2881 atggtagtag ttggtgaaaa agtcggtgcc ttcggtgctt atcgtgatgg actggaagtc
     2941 tatggccggg cgatggtttc gcaggatcgt cagttagttt cattacccga tttctcacat
     3001 tatgaactgt atgacaaacc ggaagcagtt caggaggcgc tggaaaaagt tattcctttt
     3061 ttcaacaccc atctgggata agtttcatga gaaatattat gaaaaatgta ctgattcttg
     3121 gtgctggcgg tcagatagcc cgccatgtga ttaatcaact ggcagataaa caaaccatta
     3181 aacaaacttt atttgcccgt cagccagcaa aaatccacaa accctatcca actaatagca
     3241 agattattat gggagatgta ctgaatcatg cagcactgaa gcaggccatg cagggacagg
     3301 acgttgtgta tgccaacctt acgggggaag atttagatat tcaggcaaat tcagtgatcg
     3361 ctgcgatgaa agcctgtgat gttaaacgtc tgatctttgt tctttccctc ggtatttatg
     3421 atgaagttcc aggaaaattt ggcgaatgga ataatgcagt tattggtgag cccctaaaac
     3481 cattccgcag ggctgcggat gcaattgagg cttctggact ggaatataca attcttcgcc
     3541 ctgcctggct gactgatgaa gatattattg attatgagct tacctcacgc aatgaaccat
     3601 tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt aatcacagat atcatcgata
     3661 aacctgagaa acacattggt gagaatatag gcatcaatca acccggtact gatggtgata
     3721 agccattctt catgtaaatc aattacataa ctaccatcgt actgtactat gtgcagtacg
     3781 atttattaaa cttattaagg tgaactgatc aatccgatgc gattaacatg tgtagaacca
     3841 gtaagaataa aatacgccat gtatataaca ttacgacaat tctaatatct gcacatcaat
     3901 gaaaatgcaa acaacttccg gcaaaaaagt gatacaagat cgatgacata gcgttgaaga
     3961 aaaggatttg ataatccgca aaatttgcac aacactgcct gtaatacttc ctgaaccaga
     4021 gacgatcatg accgtaaccc gcccacgcgc cgaacgcggc gcatttccgc ccggaacaga
     4081 acattacgga cgttcattat tgggcgcgcc gttaatctgg tttcctgccc ctgcagccag
     4141 tcgcgaaagt ggtttgattc tggctggcac tcacggtgat gaaaactctt cggtcgtcac
     4201 gctctcctgt gctctgcgga ctttgacacc ttctttgcgc cgccatcatg tggtgctgtg
     4261 tgtgaatcct gacggctgcc agttagggtt acgggccaat gctaatggtg tggatttaaa
     4321 cagaaacttt ccggcggcga actggaagga aggtgaaacg gtttatcgct ggaacagcgc
     4381 cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa cctggctccg aacctgaaac
     4441 ccaggcgctg tgccagctta tacaccgcat tcaacctgcc tgggtggtct cattccacga
     4501 tccactggct tgtattgaag atcccagaca cagcgaatta ggcgaatggc tgtcccaggc
     4561 gtttgaattg cctctggtaa ccagcgtcgg ctatgaaacg cctggctctt tcggcagttg
     4621 gtgtgcagat ctcaacttgc attgcatcac cgcagagttt ccgcccatct cctccgacga
     4681 agccagcgaa aaatatctct ttgcgatggc aaacttgctg cgctggcatc ctaaagatgc
     4741 aattcgcccg tcgtgaactg aagcgccggt tccacatcta ccgccagcca ggtcggtccg
     4801 tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag cggcgctaat ggcacgagag
     4861 gtacacaaca tgcagcccag catcagacga aaaccttgtg cacgtgcttc agtcgccagc
     4921 gccagcgctt ccgtcagacc cccggtttta tcgagcttaa tgttaaccat ctcatagcgc
     4981 cctttcagcg ccttcagatt gctacgagta tgacaacttt catcagcaca aatcggcaac
     5041 ggatgaataa aattctccag tgccgcatcg tcctgcgcag gaagcggttg ttcaagcatc
     5101 gcaacgccta aatccgccaa tagctggcaa cgcgccgcca acccttctgc acgccaggat
     5161 tcatttgcat caacgatcag cgtggcatcg ggcacagcgg tgcgaattgc caccatccgc
     5221 tcactgataa gatggttatc cagcttcact ttcagtaatt tcgcgcctgc ctgccagagt
     5281 gttgatgcac tattggccat ctgatcaggc gtaccgatga caaccgtctg tgcagtgata
     5341 actgtcccag gaagcgttat gccgatcaaa tcagccagcg attgctgctg ttttcgcgcc
     5401 gccagatccc acaatgcaca atccagcgca ttacgtgctg cgccggcagg gagaattttt
     5461 tgcaactcct cccgtgtcag ccctttctct aattgcggta cgacgctcat aatttgcgcc
     5521 attaccgagg catcactttc cccataacgc ggatacggcg tgcattcgcc ggtgccttta
     5581 ataccctctt cttccagttc aaccaccacc acgcgcgctt cactgcgact tccccgggca
     5641 atcacaaacg gggtatgtaa gggccaggct tcctcaaata ccttaacggt tctcatcttt
     5701 actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat agcgtttgct gtgttattga
     5761 cagttagcat aaactaggtg tgacgttaac tatatgtaaa caggaaagat aatcatgtca
     5821 caaaccgttc atttccaggg caacccggtt acagtcgcca attccatccc gcaggcgggt
     5881 agcaaagcgc agacttttac tctcgtggca aaagatctgt ctgacgtcac cctcggtcag
     5941 tttgcgggta aacgcaaagt gctgaacatt ttcccgagta ttgataccgg agtttgcgcc
     6001 gcatcagtac gtaagtttaa ccaactggca accgagatcg acaacaccgt tgtgctgtgt
     6061 atttctgccg atctgccgtt cgcccagtct cgtttctgcg gcgcagaagg tctgaacaac
     6121 gttatcaccc tctccacttt ccgtaacgct gaattcctgc aagcctacgg tgtggcaatt
     6181 gctgatggcc cactgaaagg tctggcagca cgtgccgttg tggttattga cgaaaatgac
     6241 aatgtgattt tcagccagct ggtggatgaa atcaccaccg agccggatta cgaagcagct
     6301 ctggctgtac tgaaagcata atttaatatg cctgatggtg ttgcaccatc aggcatattc
     6361 gcgcttactc ttcgttcttc ttctgactca gaccatattc ccgcaactta ttggcaatcg
     6421 cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt gctgggataa ttgcgataaa
     6481 gctgggttaa taccgagcgt tcaaaacggc tggtgatttc gtccagcgaa ccttccatcg
     6541 catcttcgcc cacggctacc gttgcggcgt cataatccgg caacaaaata tcctgtggac
     6601 gcagctcata accgtccagt tgtgtcagtg cgcgatagat agcgttcttt aactgccgca
     6661 catttcccgg ccacgcataa cgcgtaagta cagtattcag gtcagcggcc agtttcggac
     6721 gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag ctcagttaac ggcatgatgt
     6781 cctgcggaca gtcacgtagc ggcggcagat tgagcgtcaa cacgttcaga cgataataga
     6841 gatcttcacg gaacatgcct ttttgcacca gttcgaccag attcttctgc gtagcgcaaa
     6901 tcacccgcac atcgacatgc acctcatggt cttcgccaac ccgacggaaa gtgccatcat
     6961 taaggaaacg cagtaatttc gcctgcatcc gtggtgacat ctcacctatt tcatccaaca
     7021 gcaccgaacc accgttcgcc tgctcaaaga atcctttctt cccttccgga gcatgaccaa
     7081 acagttcact ctcgaccgca tcttctggta tagacgcgca attcagcgcc aggtaaggtt
     7141 tgcccgctct ggggcttgcc tgatggcagg cgtaggcaaa aagatcttta cctgtacctg
     7201 tgtcacccgt aatcagcagc ggcgcgctta gcatcgccag tttctgcgcc tgttcgacaa
     7261 catgcttcat tttcgggctg acggcgacaa tttgactaaa ggcgctgacg tcctgggcgg
     7321 caacattttg caactgacgg cccatacgaa tcgttgatcg caacatcacc accgcaccgg
     7381 tcaggacgtg ttgatcattt tcatcctgaa gataaacagg cgtaatctcc atcaggaaat
     7441 tctgcccatt aataacaaca tgctcgttat gcgaatcttg cggttcgctt tccagccaac
     7501 gtaaaaaatt aaagccgtta atcaattgtg cggcggtatg gttgcgcagg cgatccaatt
     7561 tttgcccaaa aagctgacag ctcgccgggt ttgccatatc cactttgctt ttcatatcga
     7621 cagagagcac aggttcaggc aacgcctcca gtaacgcgct caacgccaga tgctcacgtt
     7681 cggaaggcat ccacgggaca gtacgcacat cggtaacacc tgcaatacgg cgtatttcgg
     7741 ccatcagact gctgaaactc tcaaactcca gttcagcaaa attgaggtag attcgcccaa
     7801 tggggtcaat ctcaataccg cgtaaatcaa tgcctcttag cacgagtaga tcgagtaatt
     7861 cgcgggtcag accgagtcgg tcttcacaaa agacttccag acgcatggga accttcacct
     7921 gaaaaaagaa caattaatat gatgatatga cactatttgg atagcaggaa ggtttctgtc
     7981 aacaatcatt gacacaaaga cggaaaaacg ggcacttcgg cgtaaagata tccggtcagc
     8041 acccgatatt gcattatttt tcgctgggcg ttttgccttt ttgcagcgtt tctttcacct
     8101 gaccgataag ctgacgacgg aaatccccga ggcgaggttt gtcatcgtca atccacggca
     8161 gcgggcggca aagctccata gctttaatcc cgagtcgtgc cgtcagaagt ccagcaccaa
     8221 ttccctgagc tgcgcgggta gacaaacgag cagcgagatc ttgcgacatc cagtccatcc
     8281 ccacttcgcg caccagttcg ctggctccgg caaaagcgat attcagcaat accagcttaa
     8341 acagacgcaa acggctgtaa taccccagtt caatgccata cagcgtggcg atgcgattaa
     8401 ttaaacgcag attgcgccag gcgataaacg ccatatcgac caacgccagc gggctgaccg
     8461 caatcatcaa cgttgattcc gccgccgaac ggctgatttc gcgccgcgcc tgggcatcta
     8521 aaactggctg gaccaaatgc gcatacaaac tgaccacttc acggtcgttt tgcgtttcat
     8581 ggattgaggc ataccagcgt tgcagcgctg gatgagactg atcaataccc gcctgctgcg
     8641 ccagtttttc gcaaaatgcg cggcctttgc ccgtgccgtg gctgtgcaac aaatcgcgcg
     8701 cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa gcgccgccac tctgttacca
     8761 cagaacctac gccagcgccg ataatcaatg ccccagcggc acatccaccc agcgccaccc
     8821 agtcctgagt ttgccaggca ttcattgtcc actgtacacc ctgcccgaca acgcttgcgc
     8881 caaacagagc cagcccgccc atcaccattt tgcgccacag gctgcgtttc ggacgtaacg
     8941 ctgcatccat taccgcttca acttgcccct cttcttcagg cgcttcatcg agcgtggccg
     9001 gggcaaaatt ttgcgcctga ttttcgtcaa aggtctgctg cgccctgaat tttggattct
     9061 gatcgacctc cagcggaccg tcgaaatcaa tacgtggttt taacggttcg gtcatcgcaa
     9121 tttatctcct attaaaaatt caagcgcggc atcaaggcga atatgcggca gcggtttgtc
     9181 gacatccatc acctgcggac gaaacgcctc aaactggaag ccttgcttat cccagaacgc
     9241 ctgaccgggc aaacgagcgg gaacttcgcc aggataaaca gtgagcggtg cgccatcgct
     9301 gagtcgatta ccgcgtagcg ccgggatttt ctcaccgtta acatcaataa tgccgctggt
     9361 ggtcgcctgc accgaggcca gccccaggca atccatacta atcccttcaa acgccgcatt
     9421 ttgccaggca tcctgaatca gttgttgcag taatgaaacc atattggcgt gctgatcgat
     9481 ggtcacatgg tccgctttag tggcagcaaa caatagctta tcgataaccg gcgaaaacaa
     9541 acgccggaac aaggtacgct gcccgtagtg gaaactttgc atcagctgcg tcagtgccag
     9601 acgcatatca ttaaatgcct gtggcccact gttgagaggt tgcaggcaat caaccagcac
     9661 aatctggcgg tcaaagcgca gaaaatgatt cttatagaac cccttcacca ccttctcgca
     9721 gtaataatta aaccgctcgc gcagcattcc ggcattggtg tgcttatcgg cctgcgccag
     9781 tttggactcg ccccaggcat cgacatccgg ccacgggaag aattgcagcg cgggcgcacc
     9841 tgccatatct cccggcaaga caaagcgccc aggctgaata aagtgcagcc cctgctgttt
     9901 acagtggtgg agataatcgg tccacgcggc ggcaatgtct gccagccggt tttcgtcggc
     9961 aggcgctagc gggtccagcc cttcgcacat cattcgccat ttgaccgacc attcgccgcg
    10021 ctgaccattg agtaagcccg tcatctggcg tgaccagctt aaatagtcct gcgccagcat
    10081 cggcaggtcg agcaaccatt cgccgggata atccacaatc tccagataca gcgtggaggt
    10141 gtccttgaag tggcgtagca gcgaatcgtt cgatttaaaa cgtagcgcca ggcggatttc
    10201 actgacgccg cgcgttggcg tcggccaggc gggaggatcg ccatataact gcgccagtcc
    10261 ttcatcatat gtgaagcgcg gaatgccaaa gtcacgctga ggaatgcgtt ttacgcccag
    10321 caggcgctct tcacgcaccg cgcttaacag cggcaagcgt gctccggcgt gaatattgag
    10381 caactgattg accatcgcag tgataaacgc tgttttgccg ctgcggctta gtccggttac
    10441 ggcgaggcgc agatgtctgt cgacaccccg attcaccagc gcattaagtt catttttaag
    10501 tcgcttcatt gctgtcctgt gctgctctga agaccagaaa aaaatcattc gtaaggatat
    10561 gaatgtgggg gccaggccat tgattatcaa ctgcccgtgt tgtcatcgac gggcgaactc
    10621 cataagatgt gcagcattca cgttcggacg gagttcctga cctatgtccc ctactattta
    10681 tgatattgcc agggttgcag gcgtatcaaa atccaccgta tcacgcgtgc tgaataagca
    10741 aactaatatc tccccggaag cgcgcgaaaa agtgatacgg gccattgaag aattacagta
    10801 tcagccaaac aagctggccc gcgcgctgac ctcttcgggt tttgatgcca ttatggtgat
    10861 ttctacccgt tcgaccaaaa ctacggcggg taatccgttt ttctctgaag ttttgcatgc
    10921 catcaccgcc aaagctgaag aggaaggttt cgacgtgatc ttgcagacgt cgcacaatcc
    10981 ggcagaagac ttacaaaaat gtgaaagcaa aattaagcag aaaatgatta aaggcattat
    11041 tatgctaagt tcgccagcgg atgagtcatt ttttgcccaa cttgataaat acgatattcc
    11101 tgtcgtggtg attggcaaag ttgaaggtca atatgcccat gtttattctg tcgataccga
    11161 taattttggc gacagcattg cgttgaccga tgcattaatt gaaagtgggc atcaaaatat
    11221 tgcctgcctg catgctccgc ttgatgtcca tgtttcagtg gatcgggtaa atggttataa
    11281 gcagagcctg gctgcgcata atattgcagt gcgtgatgaa tggattgttg acggcggtta
    11341 tacccatgaa acagcactgc aagccgcgcg ggaattatta agccagtcac cgttgcctga
    11401 ggcagtgttt gctactgaca gcctgaaatt aatgagtatt tatcgtgcgg cagcagagaa
    11461 aaatattgct attccgcagc agttagcggt ggtgggttat agtaatgaaa cgctgtcgtt
    11521 tattttaacg cctgcgccag gcggtattga tatcccaaca caggagttag gtcaacaaag
    11581 ctgtgagtta ttattccgct taatttctgg aaaaccgtca ccacagaata ttaccgttgc
    11641 cacgcatatg acgctgaaat aaataccagc taccttgcct gaatgccgga tgcgatgcta
    11701 gcgcatctta tccggcctac atttatagtt cggataaagt actcgtgccg catccggcaa
    11761 tttagcccat tatcagaacg agtaatttac gccgacacct gcataatgga atttatcgct
    11821 gtcgccttcg tcgtgatcct gccactcaaa cgcgtattcc agcgaaacgg aaagcccgtt
    11881 ctggaaatca taaccgtaaa ataaacctac acggttaaaa tcatggcctt cacgttcaat
    11941 atcatcctgc cagtcccagt tactccagcg atccagccca atgcgcgtat acggcgtcac
    12001 cgagtggttg ccgagcgtca gcggcaaata ggcgcgaatt tcttgcgtag aaaactcacc
    12061 gttattgcgg ctgtcgtcca tattaaagcc gcgctcgaga taatagttca ctcgcaaggc
    12121 aaccgtttcg ttgaaggtat attgcaggcc tgtttccgtt tcgactcggg tatcagcgta
    12181 accggtcgtg ttcagatcgt tggcaaattt atacatcgac aaccaaccgt tgaaacgtaa
    12241 atcgtcagtc agtttcacat cccagtctgg cgcgattttc cagcgctgca tattcgccgt
    12301 gtctttaccc ggttcatcaa catagtgata accataatta cggaaaccgc cggtcaggcc
    12361 gaaactgaaa tcatcgtttt cgaggaactg ataatgcacc tccagctccg ggcgatcaaa
    12421 ccacgttcca cgtttacccg cgctataatc taccggccct tcctgataat aggccagagc
    12481 aattctccac ggcccgttgg cggcattaaa atagactgaa ggctccgcca gcccatccat
    12541 atcttcgcca taaccctcga cgttttctat ttcgtacatc gcgccgatat taaagtgcca
    12601 gtcgttcctt tcctcggcct gtgcgcaggc cattcccgca cacatcacca gtgcggtaca
    12661 gggtaataac tttttcatta ttgttccttg tattattctg ggggaatgcc ttccccctgt
    12721 atttagcgaa ttgctatttc cgtttctgca tcaaagaaat gacatttcgt catatcaaaa
    12781 tgaatagtga tattttctcc tgcatgataa tcatttaacg caccagcacg gaccactaac
    12841 tcgtgccccc caaccgtggt gtagagcata aattccgcac cggttaattc tgccacgcta
    12901 attttggcgg aaatgttatt ttcctcttgc gcgtccggat gaatatcttc cggacgtatt
    12961 cccatcacga tgggcttatg caaacttccc tgtgttttca gaaccgctaa tttctcttcg
    13021 ggaatggtta atttaagcgt ttccgtaacg aatttatcgc catcgatcgt gccacgaata
    13081 aaattcatcg ccggtgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa
    13141 accgttttcg gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc
    13201 gccatggtca tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc
    13261 tggtgcagct tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag
    13321 agcggttcat ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca
    13381 cgctggcgtt gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg
    13441 agaatttgcg ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc
    13501 ttcaggccaa acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg
    13561 aacaccatcg ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc
    13621 aacagatcgc cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta
    13681 ccgcagcccg acgggccgac aaacacgatg aactctttat cggcaatttc caggttgaag
    13741 tccttcacca catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga
    13801 gccatgttga ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag
    13861 agagtccgtt gaaggcaaca gtaattgcgc cccggttaag cccgcgccga tccccaccga
    13921 gcgcataccg ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca
    13981 tgcctgcggc ggcacgccca gcccggcaca ggcggcgaga aagatttccg ggtccggttt
    14041 cgagttttta agttgggaag catccgcgca gaaggtgaaa aactcgcgca gctccagcgc
    14101 cgctaaaatc gtcggcgcat tcagggagac agaagccagc ccaaccggga tctgctgtgc
    14161 acggagatct gccagcaaag agcgaatgcc gggtagaaca gcgctgaccg tcaactcgcg
    14221 cagtgagtgg acatatagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt
    14281 aaagtcgccc tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat
    14341 cccttttagg gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg
    14401 ctgccacgcc tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat
    14461 taccccttgc agtttcattc atcctcctga tgtgtggtag cggtcccatt gaagggcaaa
    14521 ataaaatcac ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa
    14581 cgcagtgaaa cgggcgcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt
    14641 aattcgcagc cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc
    14701 ggattgagat gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc
    14761 agccagatag cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca
    14821 ccaagatcga tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag
    14881 cgtgcggcaa caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt
    14941 tcataaaatt gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa
    15001 ttgagcatca ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa
    15061 taatccagca gaatggtttg cttccccgcc gccgctttgt atttcgccag attaatcgcc
    15121 ggcttagcca taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc
    15181 cgcagttctt taaggaacat ttcggcgcga tggataaacg catcgtcgct acagccaaac
    15241 tggcgggcaa tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg
    15301 ttattattga catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg
    15361 tcgttaaccc tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg
    15421 ccttcatgcg caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag
    15481 gcgatatcgg ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg
    15541 gtgcgaatgt taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc
    15601 cacggaaata gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag
    15661 ttgtgccagc gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac
    15721 ggtaacaaaa atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag
    15781 ccttttgccg caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa
    15841 agcgcataat ctaacgcttg ctgatcgtgc gcttcgccgc catttaccgt gatacgacgt
    15901 ttctgccacc attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc
    15961 tgcgcgcaca tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc
    16021 ctgtcatccc gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga
    16081 agctgcgcac tggtatgttg ctgcaaacgg cgctctttgg cggtatagca ttgctgcaca
    16141 tcaccgctca ccatacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg
    16201 tagatcccct gcatcagatg ctgaccaaac acccgcacct gggtttcgtc gagatgttgg
    16261 cgaccgtggt tggtttgcgt ggcgtcgata cctgttgaaa tcagcactga cgcgtcggcg
    16321 tccagtggcg taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag
    16381 cgacggctgg cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt
    16441 aattctccac tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc
    16501 tcaccattta tggcaatctc catccccacg acatcaggca ggttcatcag ttcgttgatt
    16561 tcaccttttc ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg
    16621 taatcttctt catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat
    16681 gcatacttgt tcagggtatg ctggctaaaa tggggttctg ataacgttac tggcctggtc
    16741 ataattccac acgcgtccct gtttctgcgg attgatataa cgccgccacc agttgctgga
    16801 tgatgtaccc ctgctcggcg tcggctatca tcacgggctt gccctgtacg tgattgataa
    16861 aggcttccat gctgcgcaaa tggcggttgt cgtctgccat ttcccgttgc atcagcgtca
    16921 ttaattcacc gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccggctttat
    16981 caccacaaaa gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt
    17041 ccagccacag aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc
    17101 tataagttgc cggatcccac tcgccaaatt gaccacagct cttttgcgtg ccgatctttt
    17161 gaaagctatg cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat
    17221 ccagcatatg aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa
    17281 agacgcccca gccaggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct
    17341 ctcccaaaac gccgttggtc acctggtcac gcagctgttg cgtatcgagc gcaaaacgat
    17401 ggtgaaagtc gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg
    17461 cctgttctgg cgtcatggcg ggcggttttt cgcacatcac atggcagccc gcttccagtg
    17521 ccatcagggt atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt
    17581 tcaccgccag cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg
    17641 ccagcgcctg cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc
    17701 gggtgcagta gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga
    17761 cccgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc
    17821 aggccaacga atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca
    17881 ctacatagcc ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg
    17941 tgccgctgcc cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc
    18001 ggttgtcatg cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc
    18061 cgataatctg tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg
    18121 tgttgatcat atgatcctga tagcggttta acggttcgag atacaccacg gttccggtac
    18181 gcgcggcgac ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca
    18241 ggctacgcgg cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa
    18301 cgacgatacc tttacccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta
    18361 agccattaag acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg
    18421 cggtggtcac cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga
    18481 ccagcagttt gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt
    18541 tctccagaat gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat
    18601 tcctttgaat taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata
    18661 ctgcatgtag ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca
    18721 attgagataa ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca
    18781 gcgcggataa tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt
    18841 gaaatgcgct tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcgacata
    18901 agagatggtg ccgccataag caaggccacg cagcgccgat tgcagcgcgt cggcgtaacc
    18961 gctggtttcg ataatcacat cggcaccctg cttgccggtc agcgttttga tctcttttcc
    19021 tacgtcagtg ccaatggggt taaggcagaa atcagcaccg tggcgacggg caatatcaca
    19081 gcgataggcg ataggatcga cgccaatcac caccgacgcg ccagcgcgtt tcgccagttg
    19141 gatggctatt tgaccaatcg cgccaagccc taccaccacc acaaaatcac ccacgcgcac
    19201 gttggcatcg cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt
    19261 ccacgagctg ccttgcggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac
    19321 cgtctcggag agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc
    19381 gctgccgcac tcgataatgt cgccaaccac catgttgcca agctggaatt tgccaaactc
    19441 aatgccgcgc ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc
    19501 ttcatcgata aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc
    19561 gccgaaacgc acgcggattt tcacttcatt ggctaaaatc gcccgatctt catactcaac
    19621 cagcgcagca acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag
    19681 aaactggccg ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc
    19741 cgaaagcgca tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg
    19801 tccccagatg taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt
    19861 gctggcgctg gagaggaaga tcgacgcaaa cagatagtcg ttccacgcca ccataaagca
    19921 gtagacgaac acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat
    19981 ttgcagacgg ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc
    20041 gaagtagctt ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat
    20101 cagcgccatc tcagtgtcgt aaatgcccag cgcggtgatg attttgaaca gcggcaccac
    20161 cagcaaaatg ccagagaaca tatacaccgt gtaaaagctg gcgttgatgg tcatccgacc
    20221 tttaaagcgc aggcgggaaa gcgcgtaagc cccaagaatg ccgaggaata ccgccaccac
    20281 ggatgaaacc acagacacca ccagactgtt acggaagtag tcgacaaacg ggaaaatcat
    20341 cgggttaaaa atgtcgacgt aatgctccag cgtccactgc tgcggcagca gcgtaggatg
    20401 cagtgagatc gcctctttcg cgcccttgaa cgaggtcatc agcatcacaa aaaatggaaa
    20461 cagcgtgatg atgagaaaca gcgccagccc gcagtaaaaa ccgatgcgac tgagtgtgcg
    20521 tttatttgtt gccattgagg ttcacccgtt tcctggtcag cagaatgaca gcgaaaataa
    20581 tgatgaagag caccaccgaa atcgccgccg ctttccctaa gtcattaaag gcaaaggcgg
    20641 ttttgtagag atagacgccg agaatatcga ccttggtggt cagcagataa acatcagcaa
    20701 acatgtagaa catccagata gtgcgcaggg tcaccaccgt cgccaggacc ggcataattg
    20761 cgggcagcgt gacgatgcga aaccgctgcc aggcattagc gccatccatc tctgcagctt
    20821 cgtacagcga tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag gcatacggga
    20881 agtagcgcca gatggcgaac agcaccacca gcacaaagct actgcccgga ttatcgaacc
    20941 acagcggtgc ctgctcatag agatgcagaa gatcgacgcc gaggtagttc acaatgccgt
    21001 agccgttgtt gaacatgtat ttccaggcaa acaccagcga aatggacggc gttacgtagg
    21061 agaggatcac cagcgaacgc gcggttttgc gcagatggaa ctcgcggttg aaaaacatcg
    21121 ccactgccag cccgagcacg gtgctgcccg ccaccaccag cgcggtatac cagaccgtca
    21181 tccacagcga gtgccagaag ccgggatcgg agaggatacg cacatagtta ctcaccccaa
    21241 caaaggttga ctcgatgttg ggattgagcg gcagacgtaa aaaactgatt tcgatattcg
    21301 acaccatcgg ccacgccacc agaccgccca gcagtaataa gctgggcgcg agaagcaaca
    21361 gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtccgg
    21421 ggtttagtgc tgttcgatca gttcatccag ctttttctgg ctcgcttgca gcgtgccagg
    21481 gagatcggct ttacccacgg tgacgttatg caccatggag ctcaccactc cagaccccgt
    21541 cacatcaccc atgcgggtaa agtttttatc ccctactgca ccaaagacct gaatatttgg
    21601 cagttcaccg attagctgat tcggtagttc acccagcgcc ttaataacgt cgttgtcttt
    21661 ccaggtggcg gtagtgacaa ccgctttatt caccggcagc gcagcacctg gcgacatcat
    21721 cacccagtcg gcaatgttgt ctgcctgctc cataaaggtg acaaattttt ctgctgcttc
    21781 cgtctcttcg gtcttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc
    21841 agagtttttc tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac
    21901 agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc
    21961 tttcacttcc atgatgtcgt tagaacccgg catagtgttg gcagcaaggt cgcggtaata
    22021 ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac
    22081 gttagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca cgctttctgc
    22141 tgtaggcagc gcaatgccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc
    22201 cagcagctgt tgccagtttt tcggctcctc aagccccgct tttgccagca catctttgcg
    22261 ataccagata ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc
    22321 ggtacgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac
    22381 tgtggcaacc gctttgcgat cgataagctg ctctttgtcc atcactttgg cgtagtcatg
    22441 gctggtttcg atcacttccg gcagcgaacc gctacgtgaa agagtaatga ctttagtgtt
    22501 ataggcatct tcttccacgg gcacttgctt aacggtgatg ccagggtttt ctttttcaaa
    22561 acgggcaatc aatttactga taacggcctg gcgctcctgc tccaccgaag aatgcataaa
    22621 ttcgatggat acattcgttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac
    22681 cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc
    22741 acataacctg ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca
    22801 gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg acattaaaca
    22861 atccagtcag acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa
    22921 tcgtatcaat ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa
    22981 ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc
    23041 gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat ccgagttttt
    23101 cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc gctggcacac
    23161 cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt tcttcatcgc
    23221 tactctcacg gcggcttaac gcatccatat aggtcacatt tatttcatac ggactgcgtg
    23281 taccgtcggg attatttttc cagtttacta atgcaccttc ctgctgtaac gcctcgacca
    23341 gctctaatat ttcgctttca ggcaacaagc cccgtagcgg gtttagcccg atgccatcgt
    23401 gagaggcgag gaagttaaac caggtggtgt tgctggaagg tagtgtcagg ttttgcgccc
    23461 acgcacaaag cgcctcaacg ttctgttttt gcaccgcatg cagcaccagt ggcggcagcg
    23521 agaactggta caccatatgt gcttcgtcat cgcctgcgcc aaagtaagca atgttgtctt
    23581 tatgcggaac attggtctcg gtaatgatca ctgtacctgg cgcaacgtta tcaataatcg
    23641 accgtaacag tttgataatc agatgtgttt tttccagatg gatgcagctt gttcccggct
    23701 ctttccacat aaagccaacg gcatccaggc ggacatactc agcacctttc gcaaggtaac
    23761 agagtaaaac atccaccatc gccagcaaca cttcagggct acggtaattc aggtcaattt
    23821 gatcgtcact aaaggtggtc cataaatggc gcgttgaatg atcgcgcatc tggaatggcg
    23881 ttaataacgg taacgcacgc gggcgagtga cggcgctgag atcggtttgc gggtcaacgg
    23941 caataaaaaa atcttcaaaa cctggatgct gttgtaaata gtttttaaac cattcacttt
    24001 ttgccgacat atggttgcag acaaaatcaa acattaaatg actacattca ccgagttgct
    24061 gaatatcctg ccactccccc gcttcactgg cgacctgttg ataatcaatt accgaaaagc
    24121 catcatcaga tgaccatgga taaaacggca acaaatgaac atgtgaaaaa atgctttgca
    24181 gccattgatg gtaaaactga ttaaatgtag gtaatggttt taaatcattg ctgtgaaatt
    24241 gatcggcata ggtaattaac acgacatcac tttcatccca gtgtttttta cgtcgctgtg
    24301 taattaatcg tttcgcactc tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa
    24361 atgttccacc gtagatttcg tccaggtaat ccgtaatttt ctgtttcatg ttgcctttcc
    24421 gtggtagcgg tcccatgaaa cgagagacta ctccgcagaa aataaactgt caattgagca
    24481 caagggtttt agtcacgcag gaaatcattt tttaccaggg aaaaagcgta tttgtgagct
    24541 gacgcaaata atctatgggg atgtaaataa ggtgtgacag aaaaaaaacg gcgcataagc
    24601 gccgctcatg gtgaattctt ttaacctttg accttcggca ttgcgatgtc tttcaggcca
    24661 ccggcgttct caacgtgggt atatcccatc tcgctaagga tctcttttgc ttgccctgac
    24721 tggcgtccgg cattgcaata cactttcacg gtgtcgtttt tatccggaac ggcggtggca
    24781 atgcgctctt tcacttcctt caggggaata ttgatggccc cctgaacgtg ctcttgctga
    24841 tactgctctg gaacacgaac atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac
    24901 accagtgcca gagcaagtaa gcctttttta aacatgtaag cgtccttcta tggatgaaaa
    24961 ttgccatcca ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat
    25021 aacgctgtgc cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca
    25081 acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgccgggc
    25141 catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca agctttacct
    25201 tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct
    25261 acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact
    25321 tgccgccagt tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc
    25381 aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag
    25441 ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggatacgca ccagttttac
    25501 cggtacatca aaatagttgg caatcccggc gcagacgccg cggaccatgc cctgctgtgg
    25561 aatacgccat aattttttat tgagattaat gcccgccatt agcgatccct ccagttcgga
    25621 tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca
    25681 tcagccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga
    25741 ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga
    25801 atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga
    25861 cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt
    25921 gttcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt
    25981 taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt
    26041 cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg
    26101 acgagtttgc cgcctgatga cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca
    26161 atttgttttc cagctcaccg atctctttct tcatgcgtgc cagcgtgtcg tccaccagcg
    26221 tcacttcatg ttccaggctc ttaatcagat cggtcagttt ctgtttttca attaacgctg
    26281 cacgtgccag atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa
    26341 cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccaacgcgc
    26401 gcgccgaagt agaacgtact tcaaccagtg tatcttccat ctcctggatc atcagacgaa
    26461 ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt
    26521 cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt
    26581 cctgctgaac tgttaataca atttgcgtgc caatttttta tctttttgat ttataaagat
    26641 ctgattgaag aatcaacagc aacatgccag gatgagttag cgaattacac taacaagtgg
    26701 cgaatttcat catggcagaa tacaaagata atttacttgg tgaggcgaac agctttctcg
    26761 aagtgctgga acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg
    26821 aacgcggcac cggtaaagag ctgattgcca gccgcctgca ttatctctcc tcccgttggc
    26881 aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac
    26941 tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcct gggagatttg
    27001 aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc
    27061 aggagaaatt attgcgcgtg attgagtacg gtgaactgga gcgcgttggc ggcagccagc
    27121 cattgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca
    27181 atgaaggcac ttttcgcgct gacctgctcg accgactggc ttttgatgtt gtacaactgc
    27241 caccactgcg cgagcgcgaa agcgacataa tgttgatggc agaacacttt gccatccaga
    27301 tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat
    27361 tgctgaatta tcgttggccg ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag
    27421 tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac
    27481 ggcgtccgcc tgaagacgct atcgccgttt cagaaaccac ctcgcttcca acactgccgc
    27541 tggatttacg tgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac
    27601 aagggaaata taaccagaag cgcgcggctg aattactggg gttaacttat catcagttcc
    27661 gcgcgttgtt gaaaaagcac cagatttagc gaagttgagc aatacaactt gcaggatgcg
    27721 gcgtgaatgc tttattcggc atacataatc aatacgtcgt ttgcaggcct gataagcgca
    27781 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
    27841 ttgccgcatc gaattgcgca tccggtatgg tcagcggata caggaagcga atcacgttgc
    27901 cgtatgcgcc acaggtcagc aggagcagcc cctgcgccag cgcgcgttgc tggattttct
    27961 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg
    28021 accccaggcc gcgtaccgca gcaatggccg gaacgctttc tttggcatca atcaacgtgt
    28081 ttttgagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg tcgataatat
    28141 tgagcaccgc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc
    28201 cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
    28261 cgcccgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtaa tgatccatgg
    28321 caaacagttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc atcacaatac
    28381 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
    28441 cgccctcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
    28501 atttaaacaa gcgttcgatg gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg
    28561 acggataagg tacgtgatac accgaaccag ggaacgggcc gaagccgatt ttgtacggcg
    28621 caacttttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagcca ccgctaaacg
    28681 caatcacgcc aggtcgtccg gtatgggcgc gagcaatttt caccgcgttt tccaccgctt
    28741 ccgcaccggt agtgaagaat gcggttttag cctgcccgct cactggggca agggcgttaa
    28801 ttttctccgc cagcgtgacg tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa
    28861 actgttgcaa ttgctgctcc accgccgcga ccagatcagg gtggcgatgc ccggtattca
    28921 gcaccgcaat gcctgcggcg aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtag
    28981 cgttttctgc cgactgggcg aagaagttac acatcacgcc aaccccgcgt ggggtagcag
    29041 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
    29101 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aatatgccgc gcccatcgcg
    29161 gtaaatggca cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag attggcgaag
    29221 gcatcgaaac gttcggcgtc gccgcgcagc agttcggcaa tcagacgccc ggctagatga
    29281 gtacaggtca cgccgtggcc gctgtagccc tgcatgtaat agatgttggt atcaaggcga
    29341 ccgaactgcg gcatgcgcga caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt
    29401 ttcacgccct tcagctgcgg gaaggttttc agcagtttcg gcaccacaag gcgctcaacg
    29461 tcatccgggt cgcgcgcgcc gtagaccacg ccgccgccgt acagcaggcg gttgtcggcg
    29521 gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt tttcgggatc
    29581 agcgaacggg ctaaatcttc cgacagccgt tcggtggtga tcacctgcgt gccgcacggc
    29641 atgctgcgtt tcgccagttc cggctctact ttatcgccca gatacgcatt cccggcgaca
    29701 atcacatact tcgccgtcac ctgacctttg gcagttcgca caacggctgg cgtggtgtgc
    29761 tggatctgcg tcacggcaga aagttcatac acgcgcccgc cgttgaggcg gatggcgtcc
    29821 gcttcgccaa tggcgaggtt tagcggatga atatgcccac cgctgtgatc cagcagcgca
    29881 ccggtatagc gatcgctggc tacttcacgg cgaatggcgt tggcgtccag caattccagc
    29941 tgtttattgc cgtagcgttc ccagttctct ttctgctctt caagtgtggc gagctgttta
    30001 tcattcatcg ccacaaacag gccgccgggg cggtagtcgc agtcaatctg ataacgtttg
    30061 atacgttcgc ggatgatctc accaccttcg aacatcatgc tgccgagcat acgggcggtg
    30121 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
    30181 ccgccattgc gcccgcttgc gccaaagccg atgcgtgagg cttcgagaac cactacgtca
    30241 aagcccgctt ccgccagatg cagcgcggag gagagcccgg tatagccgcc gccaaccacg
    30301 caaacgtcgc aggtgattga ctcattcagc gtgtcgaatg gcgcgtattt attcgcactg
    30361 gcggcgtaat aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
    30421 tttcagttcg gtgaattttt caagggcatg cagggatttg tcgcgaccgt tgccgctctg
    30481 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
    30541 accagccttc aggcggcggc tcatgcggtg cgcgcgggag aggtcgcgcg tccataccgc
    30601 cgcgccaagg ccgtactggc tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa
    30661 acgcgtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc
    30721 tacatccaca aagatggtcg ggccgatggc ggcagccagc tcagcgttac gaccatccag
    30781 cagcagttgc cctttgcttt cgccttcccg aataaagcta tggaccgagt cggcgtgggc
    30841 gcagtcgatt aaggtgccca tggtggttgc gggatcaagt ggatggcccg gctgccagtt
    30901 ttgcgcctgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt ccagcaacaa
    30961 acgcgttccg gcgatgcaca cctggccctg gttgtagaag atgcctgcgg cggtggcgct
    31021 tgccgctttt tgcagatccg gacaatcggc gaaaacgagg ttggcgcttt tgccgcccgc
    31081 ttccagccag acgcgtttca tgttgctgtc gcccgcatct ttcagcagct gtttcccggt
    31141 acgggttgaa ccggtaaagg caatggcgtc gatatcgtta tgacgcgaca gcgcctgccc
    31201 cgcttcatga ccaaaacctg tcaccacgtt caaaacacca tccggcaagc ctgcttcttt
    31261 cgccagcccc gcgagacgaa tcgcactgag cggtgatttt tcagacggtt tcaggacaac
    31321 gctgttccct gctgccagcg ccgggccaag cttccagcag gtcagcagca gcggaaagtt
    31381 ccacggtacg atggcggcaa tcacaccgac cggttcacgc acgatcatcg ccagctcatg
    31441 gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt accagcgaat
    31501 ggcgcgcgcc gcgccgggaa tatcatcacg cagactgtga cgaatcggtt tgccggtgtc
    31561 gagagtttcc agcagtgcca gctcttcggc gtgggcttcc attaaatcgg cgagtttatt
    31621 cagtaccgct ttacgtttag ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc
    31681 gcgtgctgcg ctcaccgcac ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag
    31741 cggtgcctgg gtgaccggat caacggtttc aaaggtttca ttttccgccg cagcagtata
    31801 ttcaccgtta ataaataagc ggttttcaat ggcgagactt aacgctttat cctgccagta
    31861 agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata tatgcagttt
    31921 gtgaacgtca gggcgcagag atccccgggc cgtatgcccg tttatttctg gcgacattct
    31981 ttatatacac ttcatccttc aagccgcctc tgccttggct gcgttcgctc ccccgaatca
    32041 cttacctgag taagctcatc gggattcgct cacttgccgc cttgatgcaa cttgaatgat
    32101 tttgtgtata gattgccggg cgcattgcgc ttacccggct tacagaacaa aaagattaaa
    32161 acgtggtggg cgtatgggcg ctgataattc ggcaaatacc tgccgacgta ttactgaaac
    32221 tgtgcgggat gccggtatta atggcataac tttgccccgc gacgagatga taatcctgac
    32281 cattaatcgt cagaacaatt tcaccttcca gtacagtgcc tatttcctca ccctgatgct
    32341 taattctttc cccagtggtt gtgccaggct ggtaagtttc aaagatcatc gccagcgtgc
    32401 gattcgggtt accgttatga accagcttca ttgacacacc ctgactgccc atctcaatta
    32461 agtcgtcctg attaatgacg acctgcggct catcaggttt ttccggctcg gaaaagaatt
    32521 ccgagagtga cagaccatac accttcagca gcttttgcag cgtactgatg gcagggctga
    32581 ctttatcttg ttctatcgta ctgatagcac tgtgagtcag cccggagagt tcggcggcac
    32641 gacgttgtga aagcccctgc tgctggcgga tttccgacaa gcgttttcct ggcgccagtc
    32701 cctcatcact catatttgca tttccttaaa ctgtagtagt cagagtcgct gtttttcagc
    32761 gatatggtgc tgacaagcgg tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc
    32821 gctactgttc cattccgggt gccactgtac gcccagcgca aaaggatgat tgatgacgct
    32881 aaccgcctca actaagccat ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac
    32941 cttcgcccct tgtccatgca gagagtttac ccaaaagttg ctacattcag gtaacaacgc
    33001 agacagtaat cccccctctt caacctgaac ttcatgcgac ggtgcatatt gctgttccac
    33061 cggcagttcg ggatcttccc gatgttccag caattcaggc tgttcgcaca gcttgcgatg
    33121 caacgaacca ccggttgcca ccaccagttc ctgtaaaccc cggcagatgg cgaaaatggg
    33181 gatgcgcctt tcgagtgcgg cattaattaa cgccatgctc agaagatcac gcccgggatc
    33241 ggcgtcaggc tcatcgccgt tttcaccata taggtgcggc tgcacattgc tgggactacc
    33301 aggaagataa atgccatcga gtttcggcaa aagttgttca agtaacgacg gttccgccag
    33361 cgcatgtggt agcgcaattg gcaagccgcc tgcgtggatg atggcattca ggtacttttc
    33421 ttgcagagtc tgggtcgcat gacccttaag cctgttcctg cacattacga caccgataac
    33481 cggattgttc attatatttt ccatgctcaa tctcacaaag tggactaaat tatcgccatt
    33541 actgcgcttt aaatgacctt tatgttcaat attttttcaa tctagcagtg ggttttcatt
    33601 tttgcaaact caatttaaca tttgacaaac atttagtttg catacagatt cgaatggtgg
    33661 tcattatatt ttacgctttg ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga
    33721 aaccaatatc gttgaagtag agaactttgt tcagcagtca gaagagaggc ggggtagcgc
    33781 ctttacgcag gaagtgaaac gctacctgga gcgctacccg aatacgcaat atgttgatgt
    33841 tttacttact gatttgaatg gttgcttccg tggaaaacgt atccctgttt caagcctgaa
    33901 gaagttggag aaagggtgtt atttcccggc ctcggtgttt gcaatggata ttctgggcaa
    33961 cgtagtagaa gaagcgggtc tgggtcagga aatgggcgag ccggatcgta cttgtgttcc
    34021 tgttctcggt tccttaaccc cttctgccgc cgatccagag tttatcggtc agatgctcct
    34081 gaccatggtc gatgaagatg gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg
    34141 cctgtggcag cagctgcgcc agcgcggatt gttcccggtc gtagcggtag agctggagtt
    34201 ctatttactg gatcgccagc gcgacgctga agggtatctg caaccgccct gcgcgcccgg
    34261 caccgatgac cgcaatacac aaagccaggt ttactccgtt gataacctca accacttcgc
    34321 cgacgtgctc aatgatattg atgaactggc gcagttacag ttaattcccg ccgacggtgc
    34381 ggtcgccgaa gcctcgccgg gtcagtttga aatcaacctt taccataccg ataacgtgct
    34441 ggaagcctgc gatgatgcac tggcgctaaa acgcctggtg cgtctgatgg cagaaaagca
    34501 taagatgcac gccactttta tggcgaagcc gtatgaagag cacgcgggca gcgggatgca
    34561 tatccatatc agtatgcaaa ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga
    34621 agattcgccg ctgctgaaaa agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat
    34681 ggcgttgctg gcaccaaacg tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc
    34741 gacgcaggcg tcgtggggcc ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga
    34801 ccgtcataat caccgcgtgg aatatcgcgt ggcgggtgcc gatgccaacc catatctggt
    34861 gatggcagcg atttttgccg gtattttgca tggccttgat aacgagctgc cgttgcagga
    34921 agaagtcgaa ggcaacgggc tggaacagga aggcttaccc ttcccgattc gccagagcga
    34981 tgccctgggt gagtttatcg agaatgatca cctgcgccgc tatttaggcg aacggttttg
    35041 ccatgtgtat cacgcctgta aaaatgatga gctgttgcag tttgagcgcc tcatcacaga
    35101 aacagaaatt gagtggatgt tgaaaaacgc gtaattcccc atgcggggtt gtttcatcgg
    35161 cctgagcgga gccaggcctg acatttgctg gtaatccaga catttcccgg atgtcgcgtt
    35221 atggagcgca ggcggcaacg ggcttccgtt aacgattaag actgtgacga ggaaatgaga
    35281 tgatgccgat gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta
    35341 ctgactggac tccgccccct ttgcttccct tgctaaacag gtttgatttc aggttgacac
    35401 gaccgcaaac cttattacgc aggggaggca gcaattatgg ctattaattc accactgaat
    35461 attgctgcgc aacccggcaa aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta
    35521 atgatgggtc tggcctatct cacgccgatg accgtatttg atacttttgg tattgtctcc
    35581 ggcattagcg acggtcacgt tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt
    35641 accgctatca gctacggcaa actggttcgc cagtttccgg aggccggttc ggcctatacc
    35701 tacgcgcaaa agtcgattaa tccgcacgtc ggatttatgg tcggctggtc atcactgctg
    35761 gattatctct ttttgccaat gatcaacgtt ttgttggcga aaatctatct ctccgccctc
    35821 ttcccggaag tgccgccgtg ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg
    35881 aatctgaaga gcgtcaacct ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc
    35941 tccatcatgg tggtgtttat cttcctggta gttcagggac tgcataaagg agaaggcgtt
    36001 ggcaccgtct ggtcacttca gccgtttatc agcgagaacg cgcacctgat cccgattatt
    36061 accggtgcga cgattgtctg tttctcgttc ctcggtttcg atgcggtgac cacgctttcg
    36121 gaagagacgc cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac
    36181 ggcggcgtta tctttatcgc ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc
    36241 cgctttaaag acccggacgc cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc
    36301 tttcagtcga ttttcctctg caccacgttt gtgaacacat tagcgtctgg cctggcttca
    36361 catgccagcg tgtcgcgtct gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc
    36421 gtgtttggct atgtgcaccc aaaatggcgg actccggcac tcaacgtcat tatggtcggg
    36481 attgtcgcgc tgtcggcgct gttctttgat ttagtcaccg cgacagcact gattaacttc
    36541 ggtgctctgg tggcgtttac cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt
    36601 aaaggaatga ataaaagctg gaaggatcac ttccactatt tgctgatgcc gctggttggc
    36661 gcgctgacgg tgggtgtgct gtgggttaac ctagagtcaa cgtcactgac actcggtctg
    36721 gtatgggctt cgttaggcgg cgcatatttg tggtatctga tccgccgcta tcgcaaagtg
    36781 ccgctgtacg aaggtgacag aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc
    36841 attatcctgc ctgagggtaa cgcgccgcgc cattaactat aaaaagatgt atgacccgga
    36901 tcacgagtgc tatcatccgc ccttatgctt agcgcattaa ccccaggggc aaataatgaa
    36961 ccacgatatc ccgcttaaat attttgatat tgccgatgag tacgcgaccg aatgcgcaga
    37021 gcctgtcgca gaggctgaac gcacaccgct ggcccactac ttccagttac tgctcacccg
    37081 tttgatgaac aatgaagaga tcagcgaaga agcccaacat gaaatggctg ctgaagcggg
    37141 gattaatccc gtacgcattg atgagatcgc agagtttttg aatcaatggg gtaatgagta
    37201 attatttaat tgcagacgat cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat
    37261 ccggctaaca atgacacgca ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct
    37321 ctgctcctcg ctaagttgtt ctgtgagtta tagttataga gaaatcacgg gtacgaatgt
    37381 tgaacagatg gcgacgccac ctgttcaacg accaaccaca cggccctcat tacgacccct
    37441 gaacacaaac ggtaatggca aatgtcatta aatattagta agatagcctc tttacataaa
    37501 atcccttaat attatgcgcc aggtattatc gtctcttttg gtgattgctg gacttgtgag
    37561 tggtcaggca atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg acagcggttt
    37621 tgtctattgc gtcagcgggc aagtcaacac ctttaaccca tccaaagcga gcagtgggtt
    37681 aattgtcgat acccttgccg cccagtttta tgatcgactg ctggatgtcg atccctatac
    37741 ctatcgcctg atgccggaac ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta
    37801 tcgcttccac ctgcgtcgcg atgttccgtt tcaaaaaacc gactggttta ctcccactcg
    37861 taaaatgaat gccgacgatg tggtgtttac cttccagcga atttttgacc gcaacaaccc
    37921 gtggcataac gtcaacggca gcaacttccc ctacttcgac agcctgcaat ttgccgataa
    37981 cgtgaaaagc gtccgcaaac tggataatca taccgttgag ttccgactcg cccagccgga
    38041 tgcttctttt ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc
    38101 ccggaagtta gagaaagaag atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg
    38161 tccgtatcag ttgtcggaat accgcgccgg gcaatttatt cgcctacaac gtcatgatga
    38221 cttctggcgc ggtaaaccgt taatgccgca ggtagtggtg gatttaggct ccggcggcac
    38281 cggacgtctg tcgaaactcc tgaccgggga atgcgacgtt ctggcctggc ctgctgccag
    38341 ccagctatcc attttgcgtg acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa
    38401 cgtcgcctat ctggcattta acaccgccaa accgccgcta aataatcccg ctgtccgcca
    38461 tgcgctggca ctggcgatta ataaccagcg cctgatgcaa tccatctatt atggtacggc
    38521 tgaaacggcg gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat
    38581 tactgaatac aatccggcga aatcgcgcga acagttgaag tcgttggggc tggaaaattt
    38641 aacgctgaaa ctgtgggtgc ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac
    38701 tgccgaactg attcaggcgg atatggcgca ggttggcgta aaagtggtga ttgtgccggt
    38761 agaaggtcgc tttcaggagg cgcggttgat ggatatgagc catgatctga cgttatccgg
    38821 ttgggcgacg gacagtaacg acccggacag tttcttccgt ccgttactga gctgcgcggc
    38881 aattcattcg cagaccaacc tcgcccactg gtgcgatccg aaattcgaca gcgtattgcg
    38941 taaggcgctc tcttcgcagc agctggcggc gcgtattgaa gcctatgacg aagcgcagag
    39001 tattctggcg caggaattgc ccattttgcc gctggcgtcg tcattgcgtt tgcaggccta
    39061 tcggtacgat atcaaaggtc tggtacttag cccgtttggt aacgcctcct ttgctggggt
    39121 gtatcgcgag aaacaggatg aggtgaaaaa accatgatta tcttcacctt acgccgcatt
    39181 ttgttattga ttgtcacctt gttcctgctg accttcgttg gcttcagctt gagctatttc
    39241 acccctcatg cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac
    39301 ggcctgatcc actgggattt tggtgtttcc agcattaacg gtcaaccgat tgccgaacag
    39361 ttaaaagagg tcttcccggc gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg
    39421 attgttggca tccccgtggg gatgattgcc gggattacgc gccataagtg gcaggataac
    39481 ttgatcaacg ccatcgcctt actgggcttt tcaatcccgg tgttctggct ggcgcttctg
    39541 ttgacgctgt tttgttcact cacgctaggt tggttgcccg tttcagggcg tttcgatctg
    39601 ctctacgaag tgaaaccgat taccggtttt gcgttgattg atgcctggct ttcggactca
    39661 ccatggcggg atgaaatgat catgagcgca atccgccata tgatattgcc cgtgatcacc
    39721 ctgtcggttg cgccaacaac tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg
    39781 tatgaccaga actatgtaaa agcggcggcg acccgcggtt tgtcgcgctt tactattttg
    39841 cgtcgccacg ttctgcataa cgcgttaccg ccggttattc ctcgcctggg cttacagttt
    39901 tccaccatgc tgacgctggc gatgatcacc gaaatggttt ttagctggcc gggtttaggg
    39961 cgctggttaa ttaacgccat ccgccagcag gattacgcag ccatttccgc cggagtgatg
    40021 gtgtgtggct cactggtcat tattgttaac gtgatttctg atattttggg tgccatggct
    40081 aaccctctga aacataagga atggtatgcc ttacgatagc gtatacagcg aaaagcgccc
    40141 gccgggcacg ctgcgtaccg cctggcgcaa attttatagt gacgcctctg cgatggtcgg
    40201 cctgtacggc tgcgcggggc tggctgtact gtgtattttt ggcggctggt ttgcgcctta
    40261 cggcatcgac cagcaatttc tcggttatca attattgccg ccgtcatggt cgcgctatgg
    40321 cgaagtttct ttcttcctgg ggactgacga cctcggacgc gatgttttaa gccgcttact
    40381 gagcggagct gcgccaaccg tgggtggcgc atttgtggtg acgcttgccg cgacgatctg
    40441 tggcctggta ctgggcacct tcgccggggc gacacacggc ctccgctcag cggtgcttaa
    40501 ccatattctg gataccttac tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc
    40561 gtttgccgga ccgagtttgt cccacgctat gtttgctgtc tggctggcat tgctgccgcg
    40621 tatggtacgt tcgatttaca gcatggtgca tgacgaactg gaaaaagagt acgttatcgc
    40681 cgcccgtctg gatggcgcat caacgctgaa tattctctgg tttgctgtga tgccaaacat
    40741 caccgccggg ttggtcaccg agatcacccg cgcactgtcg atggcaattc tcgatatcgc
    40801 cgcgctgggc tttctcgatc tcggcgcaca actcccctcg cctgaatggg gagcgatgct
    40861 cggtgatgcg ctggaactga tttatgtcgc cccgtggact gtcatgctgc caggtgcggc
    40921 aattatgatt agcgtgttgt tggttaacct ccttggcgat ggcgttcgtc gtgcgattat
    40981 tgcgggggtg gaataatgcc attactcgat attcgtaacc tgaccattga atttaaaacc
    41041 ggtgatgagt gggttaaagc cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc
    41101 cgcggtcttg ttggtgaatc cggttccggc aagagtttga ttgcgaaagc gatttgtggg
    41161 gtgaataaag ataactggcg tgttactgct gaccgtatgc gttttgatga tatcgatttg
    41221 ctgcgtctct ccgcacgcga acggcgcaaa ctggttggcc ataacgtgtc gatgattttc
    41281 caggaaccgc agtcgtgtct tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac
    41341 atcccagcct ggacctataa aggccgttgg tggcagcgtt ttggctggcg caaacgccgt
    41401 gccattgaac tgctgcaccg cgtggggatt aaagatcaca aagatgcgat gcgcagtttt
    41461 ccctatgagt tgaccgaagg tgaatgtcag aaagtgatga tagccattgc actggcgaat
    41521 caaccgcgtc tgctgattgc tgacgaaccg accaactcaa tggagccaac aacccaggcg
    41581 caaatctttc gcctgctgac gcgtctcaac caaaacagca ataccactat tttgcttatc
    41641 agccatgact tacaaatgct tagccaatgg gcggataaaa ttaacgtgct ttactgcggt
    41701 caaacagtgg aaaccgcgcc gagtaaggag ttggtgacga tgccacatca tccttatacc
    41761 caggcgctga tccgcgcgat accagacttc ggcagcgcga tgccgcataa aagtcgcctc
    41821 aatacgctgc ccggcgctat cccactgctg gaacagttac cgattggttg tcgtctgggg
    41881 ccacgttgcc cgtatgcaca acgagaatgc attgtgacgc cacgtttgac gggggcgaaa
    41941 aatcatctct atgcctgtca tttcccgctg aacatggaga aagagtgaga tgatcgaaac
    42001 gctgcttgaa gtgcgtaatt taagtaaaac gttccgctac cggaccggct ggtttcgtcg
    42061 tcagaccgta gaagcggtaa aacccttgag ctttacgcta cgtgaaggcc agacactggc
    42121 gattattggc gagaatggtt cgggtaaatc cacgctggca aaaatgctgg cgggaatgat
    42181 agagcctacc agcggcgagt tattgattga cgatcatcca ctgcattttg gcgattattc
    42241 cttccgcagt cagcgtattc gcatgatttt tcaggatcct tcgacctcgt tgaatccccg
    42301 tcaacgtatc tcgcaaattc tggattttcc actgcgcctg aacaccgacc tggaaccgga
    42361 acagcgtcgc aaacagatta ttgaaacgat gcgtatggtt gggctgctac cggatcacgt
    42421 cagttactat ccgcatatgt tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc
    42481 gctgatattg cgcccaaaag tcattattgc cgatgaagcc ctcgcttcac tggatatgtc
    42541 gatgcgttcg cagttgatta atctgatgct ggagttacag gaaaaacagg gcatttcgta
    42601 tatttatgtt acccagcata tcggaatgat gaagcacatt agcgaccagg tgctggtgat
    42661 gcatcagggc gaggttgtcg agcgaggcag caccgcggat gtgctggcat caccgctgca
    42721 tgaactcacc aaacggctga ttgccgggca ctttggcgaa gcattgacag cggatgcgtg
    42781 gcgtaaagat cgctaatttt ctgaatcctc cttccagatt attcacctca ttctgacaag
    42841 agattaaatc ttgtcagaat gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa
    42901 gaagcaatct cactttgcag gaacgaaagc tctggcgata tcttcgcagc cgacgttttg
    42961 gtgatttcaa atttcgccgt caacatccgg tggggagcta cattctcgat tttgcttgct
    43021 gctcggcgcg tgtagtcgtt gagctggatg gtgggcagca tgatttggca gttgcctatg
    43081 attccaggcg cactagctgg cttgagtcgc agggctggac cgtgctgcgt ttctggaata
    43141 acgagattga ttgtaatgag gagacggtgc tggagaatat tctgcaggaa ctgaaccgcc
    43201 ggtcaccctc tccctgaaag agcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa
    43261 aatatggtga cagttctgga gaatattcgg caaggtctga accgtcccag ccatcgccct
    43321 gaaagggtta gggggctgta taagcctgtt tgttgctggg gtgacaatat ttgcacaata
    43381 cggtcccctc gcccctctgg ggagaggatt agggtgtggg gaaaagcgcc caccctgccc
    43441 gcaccctgct ccggtcggac ctggcagcta tagccactca cagccaggtt gattataata
    43501 accgtttatc tgttcgtact gtttactaaa acgacgaatc gcctgatttt caggcacaac
    43561 aagcatcaac aataaggatt aaagctatgg gttttctttc cggtaagcgc attctggtaa
    43621 ccggtgttgc cagcaaacta tccatcgcct acggtatcgc tcaggcgatg caccgcgaag
    43681 gagctgaact ggcattcacc taccagaacg acaaactgaa aggccgcgta gaagaatttg
    43741 ccgctcaatt gggttctgac atcgttctgc agtgcgatgt tgcagaagat gccagcatcg
    43801 acaccatgtt cgctgaactg gggaaagttt ggccgaaatt tgacggtttc gtacactcta
    43861 ttggttttgc acctggcgat cagctggatg gtgactatgt taacgccgtt acccgtgaag
    43921 gcttcaaaat tgcccacgac atcagctcct acagcttcgt tgcaatggca aaagcttgcc
    43981 gctccatgct gaatccgggt tctgccctgc tgaccctttc ctaccttggc gctgagcgcg
    44041 ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc tctggaagcg aacgtgcgct
    44101 atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa cgccatctct gctggtccga
    44161 tccgtactct ggcggcctcc ggtatcaaag acttccgcaa aatgctggct cattgcgaag
    44221 ccgttacccc gattcgccgt accgttacta ttgaagatgt gggtaactcc gcggcattcc
    44281 tgtgctccga tctctctgcc ggtatctccg gtgaagtggt ccacgttgac ggcggtttca
    44341 gcatcgctgc aatgaacgaa ctcgaattga aataatcatt ctgttggtaa atattgggcg
    44401 gcgttctgcc gcccgttatc tctgttatac ctttctgata tttgttatcg ccgatccgtc
    44461 tttctcccct tcccgccttg cgtcaggata acgatttcct ttacgaccaa ggagcgccca
    44521 tggaacaacg ccacatcacc ggcaaaagcc actggtatca tgaaacgcaa tccagtactg
    44581 cggagtatga cgttctgcct ctggtcccgg aagccgcaaa ggtcagcgat ccctttctgc
    44641 tcgacgtgat ccttgatgaa gaaacgctgg ctcccttcct ttcatggctg gtccctgcgc
    44701 gcgttcttgc agtggaattg ttccctgacc agcttaccgt gacccgttca cagaccttca
    44761 ccgcttatga acgcttgtct acggccctga cggttgctca ggtttgcggc gtccagcggt
    44821 tatgtaacta ctattcggcg cgacttacgc cgctccccgg gcctgattcc tccagggaaa
    44881 gcaatcatag gttggcacaa atcacgcaat atgcccgcca actggctagc tcgccttcta
    44941 ttatcgacaa ccgatcgcgc cagcatctga atgacgtcgg tcttactgcc ggggactgtg
    45001 tgatcattaa ccaaatcatt ggttttattg gctttcaggc gcggaccatt gcgacatttc
    45061 aggcttatct cgggcacccg gtacgctggt tacccggtct ggaaatacaa aactacgccg
    45121 acgcgtcact gtttgctgat gaatcaatac gctggcgaag cagctatgag gtggaaaaac
    45181 tacctgaaga gtacacaaaa agttcaacag cagaactttg ccaactggcc gaaacactct
    45241 ctctccaccc tatttcactt tcccttctcg aaaggttgtt aaacagtaca cgggttaata
    45301 cacagccgga taataagctt gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt
    45361 gttttgccgc ctgtatggat tcatcaaatg aatataaaaa aatcagcccc cttctgcgca
    45421 agggcgaaaa tgaaattaac caatgggctg accgtcattc tgttgagcac gctaccgttc
    45481 aggcgataca atggctgacc cgagcacccg atcgctttag cgccgcccag ttcagccctt
    45541 tactcgaaca cgaaaaatca tcaacgcaga ttattaatct gctggtatgg agcgggctgt
    45601 gtggctggat aaatcgctta aaaatcgcgt tgggtgagac atattaacct tgccgcgtca
    45661 gacagattcg cgtaaaactg tcagccgctc taatggccac caaaatagac aattatgttt
    45721 caggacaacc cgctgctagc gcagcttaaa cagcaactgc attcccagac gccacgcgct
    45781 gaaggggtgg taaaagccac agaaaaaggc tttggcttcc tggaagtcga cgcgcaaaaa
    45841 agttatttca ttccgccgcc gcagatgaaa aaagtcatgc atggcgaccg aattatcgcg
    45901 gtgatccaca gtgaaaaaga acgtgaatcc gcagagccag aagaactggt tgaaccgttc
    45961 ctgactcgtt tcgtgggtaa agttcagggc aaaaatgacc gtctggccat cgttcctgat
    46021 catccactct taaaagacgc cattccttgc cgcgcagccc gtggcctgaa ccacgagttt
    46081 aaagaaggcg actgggcggt tgccgaaatg cgccgtcatc cgctgaaagg cgatcgttct
    46141 ttctatgctg aactgacaca atacatcact tttggtgacg accactttgt accgtggtgg
    46201 gttacccttg cgcgccataa tctggaaaaa gaagcaccag acggcgtcgc taccgaaatg
    46261 ctcgatgaag gtctggttcg tgaagatctg accgcgctgg attttgtcac catcgacagt
    46321 gccagcacag aagatatgga tgacgccctt ttcgctaagg cgttgccgga tgacaaactt
    46381 cagctgattg tggcgattgc cgatccaacc gcgtggattg ctgaaggcag caagctggac
    46441 aaagccgcga aaattcgcgc attcaccaac tatctgcctg gcttcaacat ccctatgctg
    46501 cctcgcgagc tttctgacga tctctgctca ctgcgcgcca atgaagtccg cccggtactg
    46561 gcatgccgca tgacgctctc cgctgatggc accattgaag ataatatcga attctttgcc
    46621 gccaccatcg aatccaaagc aaagctggtg tatgaccagg tttctgactg gctggaaaat
    46681 accggtgact ggcagcctga aagtgaagca attgccgaac aagtccgttt gctagcgcaa
    46741 atttgccaac gccgcggcga gtggcgtcat aaccacgcac tggtgtttaa agatcgcccg
    46801 gattaccgct ttattctcgg tgaaaaaggt gaagtgctgg atatcgtcgc cgagcctcgt
    46861 cgcattgcca accgtatcgt cgaagaagcg atgattgccg ctaacatttg tgcggcccgc
    46921 gtactgcgcg ataagctcgg ttttggcatc tataacgtgc atatgggctt tgatccggcg
    46981 aatgccgacg cgctggcagc gttgctgaaa acgcacggtc tgcatgtcga tgccgaagaa
    47041 gtgctcacgc tggacggttt ctgcaaactg cgtcgtgaac tggatgcgca accaactggt
    47101 ttcctcgaca gccgcattcg tcgcttccag tcatttgctg aaattagcac tgaacccggt
    47161 cctcactttg gcctcggtct ggaagcatac gccacctgga cttcgccgat ccgtaaatat
    47221 ggcgacatga tcaaccaccg tctgctgaaa gcggttatca aaggcgaaac tgcgacgcgc
    47281 ccacaggatg aaatcactgt ccaaatggcc gagcgtcgcc gtctcaaccg gatggcagaa
    47341 cgtgatgttg gcgactggtt atacgcacgc ttcctgaaag acaaagccgg gaccgacacc
    47401 cgtttcgcgg cggaaattgt cgatatcagc cgtggcggca tgcgtgtgcg tttggttgat
    47461 aacggcgcta tcgcctttat tccggcacct ttcttacacg ctatgcgcga tgaactggtt
    47521 tgcagccagg aaaacggcac tgtacaaatt aaaggtgaaa cggtttacaa agtaactgac
    47581 gttattgacg tcaccattgc cgaagtccgc atggaaaccc gcagcattat tgcgcgcccg
    47641 gtcgcgtaat ctcctttcac ggcccattcc tcatggatgg gccgtttatt tccccgctct
    47701 accttcgtta tatccaggca gattttttaa ctgactttcg tttgaaaact ggcgtttttc
    47761 caaaactgga ttacaaataa atatataaaa aaaacaatgc gtttagccgc cgttaatttc
    47821 atgtaagtca tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg
    47881 catcatgaaa accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc
    47941 atactggcgg ttgacggaaa gcagcgatgt tttgcgcttt tctaccaccg aaaccacaga
    48001 acctgatcgt acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt
    48061 catcacctcc agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct
    48121 ggtaggacga aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac
    48181 accggcggtt gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga
    48241 agcacattcc gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt
    48301 atgtgaagat tacactgggt tgaaagaaca cgacgtcatt ggccaaagcg tgtttaaact
    48361 gtttatgagc cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag
    48421 cggcaatgca tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct
    48481 gtttcgcaat aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc
    48541 cggcaccgac attaccgaag agcgccgcgc tcaggagcga ctgcgtattc tggcaaatac
    48601 cgacagtatc accggactgc cgaatcgtaa cgcaatgcag gatttaatcg atcacgctat
    48661 taatcatgca gataacaata aagttggggt tgtgtatctt gatttggata atttcaaaaa
    48721 ggtcaacgac gcctatgggc atttgtttgg tgaccagtta ttacgcgacg tgtcattggc
    48781 tattttaagc tgtctcgaac atgaccaggt gttggcgcgt ccaggtggcg atgagtttct
    48841 ggtactggca tccaacacct cacaaagcgc gctggaagca atggcatcac gaattttgac
    48901 ccgcttacgg ctcccctttc gcattggttt aattgaagtt tataccagct gttcagtagg
    48961 tattgcactc tctcccgaac atggttcaga cagcacggct attattcgtc acgccgacac
    49021 agcaatgtac acagcgaagg aaggcggacg aggacaattt tgcgttttta ccccagaaat
    49081 gaatcaacgg gtatttgaat atctctggct ggataccaac ttgcgtaaag cactggaaaa
    49141 cgatcagttg gttattcact atcaaccgaa aatcacctgg cgtggcgaag tgcgcagtct
    49201 ggaagcacta gtacgttggc agtcacctga acgtgggttg attccaccgt tggacttcat
    49261 ttcctacgcc gaagagtcag ggctaattgt gcctttaggc cgttgggtga ttctcgatgt
    49321 cgtacgccag gtggcgaagt ggcgggataa aggtattaac ctgcgagtgg cggtaaatat
    49381 ttctgcacgt cagctcgccg atcaaaccat tttcaccgcc ctgaaacagg ttctccagga
    49441 actcaatttt gaatactgcc ctatagatgt tgaactgaca gagagttgtc tgattgagaa
    49501 tgatgaactg gcactgtctg ttattcaaca atttagccaa ctaggtgcgc aagtgcatct
    49561 ggatgatttt ggtaccggct actcttcact ttcgcaactg gcgcgctttc cgatcgatgc
    49621 catcaaactt gaccaggttt ttgttcgaga tattcacaaa caacctgtct cgcagtcact
    49681 ggtccgggcg atcgtcgctg tggcccaggc attgaatctt caggtgatcg ccgaaggtgt
    49741 agagagtgca aaggaagatg cttttttaac caagaacggg atcaatgagc ggcaaggatt
    49801 tttgtttgcc aaaccgatgc ccgccgtcgc cttcgaacgc tggtataaac gctatctgaa
    49861 gcgcgcataa gtagatgaat atatatactt agcgctatat cccgtcatta tatatcgtat
    49921 tcctggatga taattagccg ttgctgttaa acacttctct tttagcggca atttttgcat
    49981 tattcatttt ccacttttgc tataaggatg tccttgatgt ctgaatttga tgcccagcgc
    50041 gttgccgaac gtattgatat tgtgttagat atccttgtgg caggcgatta ccactctgcg
    50101 atccataatc tcgagatact taaagcagaa ctcttgcgcc aggttgctga atctacgcca
    50161 gacatcccga aggcaccgtg ggagatttaa caatcgtgcc ttcaggtttt cctatttgag
    50221 gatgcccggc gcgtcagtat ttttttattt agtattataa cgttataaga attacagcga
    50281 tgaattcccg acaacaaact attctacaga tggtcattga ccagggtcag gttagcgtaa
    50341 ccgatctggc aaaagccact ggagtttctg aagttaccat tcgccaggat ctcaacaccc
    50401 tcgaaaaact gagttacctc cgccgtgcac atggctttgc agtttcgctt gatagtgatg
    50461 acgtcgaaac ccgtatgatg agcaactata cgctgaagcg tgaactcgcc gagtttgccg
    50521 cgtcactggt tcaaccgggc gaaaccatct ttatcgaaaa tggtagcagc aatgccctgc
    50581 tcgcccggac tctgggcgaa cagaagaaaa atgtcactat catcacagtc agcagctaca
    50641 ttgcgcattt gctgaaagac gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga
    50701 aaaaaagcga aagtatggtt ggccctttga cacgccagtg catccagcag gtatatttca
    50761 gcaaagcatt tattggtatt gatggctggc aacctgaaac tggattcacc ggtcgcgata
    50821 tgatgcgtac cgatgtggtc aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga
    50881 ctgacagctc gaaatttggt gctgtacatt catactccat cggtcccgtt gagcgattca
    50941 atcgcgtgat taccgattcg aaaatacgcg ccagcgatct gatgcatctt gagcacagta
    51001 aactcaccgt tcacgtcgtt gacatttaac catttccctt ctctacggat gatttgcagt
    51061 ttggcaaatc atccgctcta agatgattcc tggttgataa ttaagactat ttacctgtta
    51121 ttaacactc
//
LOCUS       DAPHDX010000012        50061 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPHDX010000012 DAPHDX010000000
VERSION     DAPHDX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.012"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(14..661)
                     /locus_tag="RH058_000706"
     CDS             complement(14..661)
                     /locus_tag="RH058_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3136342.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            complement(863..1567)
                     /locus_tag="RH058_000707"
     CDS             complement(863..1567)
                     /locus_tag="RH058_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3136343.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1704..2156)
                     /gene="moaE"
                     /locus_tag="RH058_000708"
     CDS             complement(1704..2156)
                     /gene="moaE"
                     /locus_tag="RH058_000708"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV3136344.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(2158..2403)
                     /gene="moaD"
                     /locus_tag="RH058_000709"
     CDS             complement(2158..2403)
                     /gene="moaD"
                     /locus_tag="RH058_000709"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV3136345.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMSAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2396..2881)
                     /gene="moaC"
                     /locus_tag="RH058_000710"
     CDS             complement(2396..2881)
                     /gene="moaC"
                     /locus_tag="RH058_000710"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV3136346.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2884..3396)
                     /gene="moaB"
                     /locus_tag="RH058_000711"
     CDS             complement(2884..3396)
                     /gene="moaB"
                     /locus_tag="RH058_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV3136347.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3418..4407)
                     /gene="moaA"
                     /locus_tag="RH058_000712"
     CDS             complement(3418..4407)
                     /gene="moaA"
                     /locus_tag="RH058_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV3136348.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4395..4539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4804..5712
                     /gene="yvcK"
                     /locus_tag="RH058_000713"
     CDS             4804..5712
                     /gene="yvcK"
                     /locus_tag="RH058_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV3136349.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5904..7925)
                     /gene="uvrB"
                     /locus_tag="RH058_000714"
     CDS             complement(5904..7925)
                     /gene="uvrB"
                     /locus_tag="RH058_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV3136350.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8504..9181)
                     /gene="bioD"
                     /locus_tag="RH058_000715"
     CDS             complement(8504..9181)
                     /gene="bioD"
                     /locus_tag="RH058_000715"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV3136351.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(9174..9929)
                     /gene="bioC"
                     /locus_tag="RH058_000716"
     CDS             complement(9174..9929)
                     /gene="bioC"
                     /locus_tag="RH058_000716"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV3136352.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9916..11070)
                     /gene="bioF"
                     /locus_tag="RH058_000717"
     CDS             complement(9916..11070)
                     /gene="bioF"
                     /locus_tag="RH058_000717"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV3136353.1"
                     /translation="MSWQEKINAALAARRTTDALRRRYPVTQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPHIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPAQLRRFAHNDVTHLAR
                     LLAASCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            complement(11067..12107)
                     /gene="bioB"
                     /locus_tag="RH058_000718"
     CDS             complement(11067..12107)
                     /gene="bioB"
                     /locus_tag="RH058_000718"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV3136354.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLRQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            12194..13483
                     /gene="bioA"
                     /locus_tag="RH058_000719"
     CDS             12194..13483
                     /gene="bioA"
                     /locus_tag="RH058_000719"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV3136355.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHTPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARNAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13542..14018
                     /locus_tag="RH058_000720"
     CDS             13542..14018
                     /locus_tag="RH058_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV3136356.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            14170..15453
                     /locus_tag="RH058_000721"
     CDS             14170..15453
                     /locus_tag="RH058_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV3136357.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15687..17948)
                     /locus_tag="RH058_000722"
     CDS             complement(15687..17948)
                     /locus_tag="RH058_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV3136358.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(18131..19564)
                     /locus_tag="RH058_000723"
     CDS             complement(18131..19564)
                     /locus_tag="RH058_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV3136359.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(19640..20692)
                     /locus_tag="RH058_000724"
     CDS             complement(19640..20692)
                     /locus_tag="RH058_000724"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV3136360.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20876..21829
                     /locus_tag="RH058_000725"
     CDS             20876..21829
                     /locus_tag="RH058_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136361.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(21870..22865)
                     /gene="pgl"
                     /locus_tag="RH058_000726"
     CDS             complement(21870..22865)
                     /gene="pgl"
                     /locus_tag="RH058_000726"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV3136362.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            23020..23838
                     /gene="ybhA"
                     /locus_tag="RH058_000727"
     CDS             23020..23838
                     /gene="ybhA"
                     /locus_tag="RH058_000727"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV3136363.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(23839..24897)
                     /gene="modC"
                     /locus_tag="RH058_000728"
     CDS             complement(23839..24897)
                     /gene="modC"
                     /locus_tag="RH058_000728"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV3136364.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(24900..25589)
                     /gene="modB"
                     /locus_tag="RH058_000729"
     CDS             complement(24900..25589)
                     /gene="modB"
                     /locus_tag="RH058_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV3136365.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(25589..26362)
                     /gene="modA"
                     /locus_tag="RH058_000730"
     CDS             complement(25589..26362)
                     /gene="modA"
                     /locus_tag="RH058_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3136366.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(26529..26678)
                     /gene="acrZ"
                     /locus_tag="RH058_000731"
     CDS             complement(26529..26678)
                     /gene="acrZ"
                     /locus_tag="RH058_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV3136367.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            26807..27595
                     /gene="modE"
                     /locus_tag="RH058_000732"
     CDS             26807..27595
                     /gene="modE"
                     /locus_tag="RH058_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV3136368.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIEHGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            27663..29135
                     /gene="modF"
                     /locus_tag="RH058_000733"
     CDS             27663..29135
                     /gene="modF"
                     /locus_tag="RH058_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV3136369.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            29396..30412
                     /gene="galE"
                     /locus_tag="RH058_000734"
     CDS             29396..30412
                     /gene="galE"
                     /locus_tag="RH058_000734"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV3136370.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            30422..31468
                     /gene="galT"
                     /locus_tag="RH058_000735"
     CDS             30422..31468
                     /gene="galT"
                     /locus_tag="RH058_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV3136371.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            31472..32620
                     /gene="galK"
                     /locus_tag="RH058_000736"
     CDS             31472..32620
                     /gene="galK"
                     /locus_tag="RH058_000736"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV3136372.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPHDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            32614..33654
                     /gene="galM"
                     /locus_tag="RH058_000737"
     CDS             32614..33654
                     /gene="galM"
                     /locus_tag="RH058_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV3136373.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVART
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            33859..34611
                     /gene="gpmA"
                     /locus_tag="RH058_000738"
     CDS             33859..34611
                     /gene="gpmA"
                     /locus_tag="RH058_000738"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV3136374.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(34769..35821)
                     /gene="aroG"
                     /locus_tag="RH058_000739"
     CDS             complement(34769..35821)
                     /gene="aroG"
                     /locus_tag="RH058_000739"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV3136375.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            36137..36517
                     /locus_tag="RH058_000740"
     CDS             36137..36517
                     /locus_tag="RH058_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV3136376.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            36631..37572
                     /gene="zitB"
                     /locus_tag="RH058_000741"
     CDS             36631..37572
                     /gene="zitB"
                     /locus_tag="RH058_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV3136377.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHEHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(37569..38288)
                     /gene="pnuC"
                     /locus_tag="RH058_000742"
     CDS             complement(37569..38288)
                     /gene="pnuC"
                     /locus_tag="RH058_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV3136378.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(38326..39369)
                     /gene="nadA"
                     /locus_tag="RH058_000743"
     CDS             complement(38326..39369)
                     /gene="nadA"
                     /locus_tag="RH058_000743"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV3136379.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(39618..39693)
                     /locus_tag="RH058_000744"
     tRNA            complement(39618..39693)
                     /locus_tag="RH058_000744"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39658..39660),aa:Lys,seq:ttt)
     gene            complement(39727..39802)
                     /locus_tag="RH058_000745"
     tRNA            complement(39727..39802)
                     /locus_tag="RH058_000745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39767..39769),aa:Lys,seq:ttt)
     gene            complement(39949..40024)
                     /locus_tag="RH058_000746"
     tRNA            complement(39949..40024)
                     /locus_tag="RH058_000746"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39989..39991),aa:Lys,seq:ttt)
     gene            complement(40028..40103)
                     /locus_tag="RH058_000747"
     tRNA            complement(40028..40103)
                     /locus_tag="RH058_000747"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40068..40070),aa:Val,seq:tac)
     gene            complement(40253..40328)
                     /locus_tag="RH058_000748"
     tRNA            complement(40253..40328)
                     /locus_tag="RH058_000748"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40293..40295),aa:Lys,seq:ttt)
     gene            complement(40331..40406)
                     /locus_tag="RH058_000749"
     tRNA            complement(40331..40406)
                     /locus_tag="RH058_000749"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40371..40373),aa:Val,seq:tac)
     gene            complement(40542..40617)
                     /locus_tag="RH058_000750"
     tRNA            complement(40542..40617)
                     /locus_tag="RH058_000750"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40582..40584),aa:Lys,seq:ttt)
     gene            complement(40782..41573)
                     /gene="cpoB"
                     /locus_tag="RH058_000751"
     CDS             complement(40782..41573)
                     /gene="cpoB"
                     /locus_tag="RH058_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV3136380.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(41583..42104)
                     /gene="pal"
                     /locus_tag="RH058_000752"
     CDS             complement(41583..42104)
                     /gene="pal"
                     /locus_tag="RH058_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV3136381.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(42139..43431)
                     /gene="tolB"
                     /locus_tag="RH058_000753"
     CDS             complement(42139..43431)
                     /gene="tolB"
                     /locus_tag="RH058_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV3136382.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(43564..44829)
                     /gene="tolA"
                     /locus_tag="RH058_000754"
     CDS             complement(43564..44829)
                     /gene="tolA"
                     /locus_tag="RH058_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV3136383.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(44894..45322)
                     /gene="tolR"
                     /locus_tag="RH058_000755"
     CDS             complement(44894..45322)
                     /gene="tolR"
                     /locus_tag="RH058_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV3136384.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(45326..46018)
                     /gene="tolQ"
                     /locus_tag="RH058_000756"
     CDS             complement(45326..46018)
                     /gene="tolQ"
                     /locus_tag="RH058_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV3136385.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(46015..46419)
                     /gene="ybgC"
                     /locus_tag="RH058_000757"
     CDS             complement(46015..46419)
                     /gene="ybgC"
                     /locus_tag="RH058_000757"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV3136386.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(46569..46862)
                     /gene="ybgE"
                     /locus_tag="RH058_000758"
     CDS             complement(46569..46862)
                     /gene="ybgE"
                     /locus_tag="RH058_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV3136387.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(46862..46975)
                     /gene="cydX"
                     /locus_tag="RH058_000759"
     CDS             complement(46862..46975)
                     /gene="cydX"
                     /locus_tag="RH058_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV3136388.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(46990..48129)
                     /gene="cydB"
                     /locus_tag="RH058_000760"
     CDS             complement(46990..48129)
                     /gene="cydB"
                     /locus_tag="RH058_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV3136389.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(48145..49713)
                     /gene="cydA"
                     /locus_tag="RH058_000761"
     CDS             complement(48145..49713)
                     /gene="cydA"
                     /locus_tag="RH058_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV3136390.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        12321 a        13334 c        12406 g        12000 t
ORIGIN      
        1 tcgtcaattc gcactaccac actaaaccat taataaattc aagaactgtt acagtaattc
       61 taaagaggac gcttaaaata ctaaaataga gtgttattgc cccaggaacg atacagcgaa
      121 gagaacctga aatttcagag gtgtcggcaa cacggatcaa atcttccagt gttgttgctt
      181 cacaggcagc cgtaatgccc cagataaaga cagtcaaaat actcgctaac cacacggaaa
      241 aactgctggt aaggatcagg ttaacaacaa aagcaatact caaaccacag aaaatatatg
      301 aatataactg tctgactggt gggataaccc tattactaca agagcaaatc agcgccattg
      361 cggcaaacat ccctcccgta attcccatta ccgtcgctat gctcgaaaag gtataaaggc
      421 tggcgaagag aaagccaaca agaaggatta ttagcagata gaccagacat gcgcctgata
      481 cattcagtct tttcagggcg ttgtttaacc agagaccacc tattatcagc gcagccaaag
      541 cgaagacaaa cgcacctgga gacttttgtg acgtaaccca atgcaaatgc tgaaatatcc
      601 aggcacagcc tgccgtagcc catactccag ctgctgttaa ccaccaaatg agaagcagca
      661 ttttgctgag cgaagcactt ttttgctgta attgagtagc agtggattgt ctgttcaaat
      721 gcgttatctg tccctgaaat tacgtagtgg acatagtctg gcatttgccg cgattcagtt
      781 ctcatgtaaa tgagcggaat agtctttata aaatccgaaa aaaatcccgg ctccaggccg
      841 ggatcagcag gaagaagaga atttaacggc ggttgccgaa gatccgcaac aacatcagga
      901 acaggttgat gaagtccaga tacaaggtta acgcgccaag aatggaatat ttgcgcaggt
      961 tcgacgtgtc gcgggtatca atctgctcac ccatattttt cagtttctgc gtgtcatacg
     1021 ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac tgcccacatc aatgcttcgc
     1081 ttttcaacca gaagttgacc agcgatgcca gcacaatgcc gattaacgcc ataaacagca
     1141 tattgccaaa gccacttaaa tcgcgcttcg tggtgtaacc gtacaggctc attgcgccga
     1201 acatcccggc agtaacgacg aaagtactgg cgatagaagc agcggtatag acaatgaata
     1261 tactggaaag cgtaagaccc gtcagcgccg aataaagcat aaagagcatc gtcgttacac
     1321 ctgcgctcag cttttgaatc atcgctgata acacaataac caatgctaat tgcgcgatga
     1381 tcagaccgat taaaaagaca cggttagtga acaacagctc catcacggcc gcggaattag
     1441 ccgcatacca ggcaacaaat gcggtcagca acaagccaac ggtcatccag ccatagactt
     1501 gcgccatata agtttgcaag ccagcccggg gttgtacgat tgaatcagaa cgtgggaatc
     1561 tgtccatgac gatctcctga agatataagg aatatcttaa ggatactgca aaatgatgag
     1621 gctgtgcatc gacgcagcgt aaacgtatgt actgagcggt gaaattgccg gacgcagcgg
     1681 tgccttatcc ggctaacaaa aaactaccag cgttttgccg cctgctgatc gctctcccgg
     1741 gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct tccagaacgg tgcgcgggtt
     1801 ttgagataat ccataataaa ctgcccggct tcaaacgcac tgctgcgatg cgcactggtg
     1861 acaccgacaa aaacgatttc atcgcccggc cataattccc cgatgcggtg aatcacagtg
     1921 acgcgcccca gcggccagcg gttacgcgct tcatcaacaa tttctgccag tgctttttca
     1981 gtcatccccg gatagtgttc gagggttaat gctttgacgc tgtcgcccag gttatggttg
     2041 cgcaccttac cagtaaaggt gactaccgca ccgtcttcgt cacgctccgc cagccacggg
     2101 tactcttctc ctacgctgaa cggctgcgga ccaacaacaa ttttggtttc tgccatctta
     2161 acctccggtt accggcggga agaaagctac ttcgtcgccg tcagtcagcg gatggtcaaa
     2221 actcaccagc gtctggttga cggcagccag taatttgcca tcttccagcg ccagcgccca
     2281 gcgatcgctc tgcgcagaca tgtgctggcg taacgcttca acagttggga aatccgcagc
     2341 cacttcggtt gcatctgttc ccaccaactc gcgcacctgg gcgaaaaaaa gaactttaat
     2401 catccgcttc caccttaaag tcacccgact tgccgccgct cttcgccagc aaacgtaccg
     2461 gaccaatcac catatctttt tgcaccgctt tgcacatgtc ataaatggtc agcgccgcca
     2521 cggaggccgc ggttaatgct tccatttcga caccggtttt cccggtcagg cggcataagg
     2581 tttctatacg cacccgattg tgctccggct cggcctgtaa attgacttca actttgctga
     2641 gcatcagcgg atgacagagc gggatcagat cccaggtgcg ttttgccgcc tgaataccgg
     2701 caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg accatcaata atcatcgcca
     2761 gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc ttcacgcacg gtttccgctt
     2821 tggcggagac atccaccatg tgcgcttcgc cagcggcgtt gatatgggtc agttgcgaca
     2881 tacttatttc ttcaaatgtg gatggaaatt acacggacgc gtacgggcat ccagctgcgg
     2941 cgcgatgata ttttcccatg cggtacggca cgctttggtc gaacccggca tggcgaaaat
     3001 cagcgttttg ttggcgacgc ccgctaccgc acgagattgc aacgtggaag tgccaatctc
     3061 ttcaaaagac aacatccgga acacttcacc aaaaccttca acttcacggt cgaacaacgg
     3121 cagcaatgct tcgggagcct gatcaccttc cgtcaggcca gtaccacccg taatcaatac
     3181 cacttgtaca tcgtcgctgg cgatccacgc agatacctga gcgcgaatag cgtagcggtt
     3241 ttctttcaca atggctttat cgacaacgtg atggcccgct tcttgcgccg aatcgcgcag
     3301 atagtgaccg gaggtatcgt cttcttcacc gcgacgatta gaaaccgtaa gaatagcaat
     3361 acgggtcggg ataaattcag tgcttacctg actcatctga tctctccttt tgacgtttta
     3421 gccgccaatg tacgataagt tttgcgtaat accggtgttg ttttgatgca ggaaatgggt
     3481 ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc agcgcctgtt gctgggtatc
     3541 gtcttccagc agatcgcgca ggttaacgcc gccttcacca aacaggcaga gatggagttt
     3601 accaatggag gaaacgcgca ggcggttgca agtggcgcag aagtcttttt catacggcat
     3661 gataaggcca atctctccgg cgtaatctgg atggcaaaag acttgcgcgg gaccgtcgct
     3721 gcgttgacgt aattggtgga tccagccgcg acgcagtagc tcgtcacgca gaacctgacc
     3781 agagatgtga tgcttacgga agagctcgct gccctcgccc gtttccatca gttcgatgaa
     3841 acgcagctgg atagggcgat gctggatcca gttcagaaag gtgtcgagct ggtgatgatt
     3901 aacatcacgc atcagcacgg tattgacttt gaccttctca aaaccggcct caaatgcagc
     3961 atcaatcccc gccatgacct ggttgaattt atcctgcccg gtaatagcgt gaaactggcg
     4021 ggcgtccaga ctgtcgacac tgacgttaat gccagtaagt cccgcatcgc gccagttcgc
     4081 cacatcgcgt tccagacggt aaccattggt ggtgaccgca atctggcgga tagcgtcgtt
     4141 ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt aaagacggct caccgcctgt
     4201 caggcgcact ttttcggtgc ccagactggc gaaggcgcgc gtaacccggc gaatttcatc
     4261 gacggtaaga aagcctttat tggtgacgcc gctcggtttg tagccatccg gcaggcagta
     4321 ggtgcaacga aagttacaca catcggtaat cgacaggcgc aagtagtaaa acttacgcgc
     4381 aaatgcatca gtcagttgtg aagccatgta cacctttcca gatacgggag gcgaagtcat
     4441 ttcttccttc gccctggtgg caatctttac gagcattgtc acggccaaag caccgtatca
     4501 gttgacccag gtgcagaggc tagagtgttt agtggttatg ccgatactag cgtgtaaatg
     4561 agatttttac catccacact ttcgctatgt aatcatgtat atagcgtcat gatcgtgcta
     4621 tttcgctaca gaataaggga aaatggcggt tttactttga tatacatcat cagggaggca
     4681 tagcgtgcat cgtcctttag aggtagaaga aaatagtttg tatcctcaat attggcaggt
     4741 taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg ataatctgac tccaggaaac
     4801 tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg ttgctctcgg cggagggcat
     4861 ggactgggac gcgttctctc atcactttcg tctttgggtt ctcgtttaac gggtatcgtc
     4921 accaccaccg ataatggtgg ctcgacgggg cgtattcgcc gttcagaagg cggcattgcc
     4981 tggggcgata tgcgcaactg cctcaaccag ctgataacgg aaccgagcgt cgcctccgcg
     5041 atgtttgaat accgttttgg tggcaatggc gaactttccg gtcataatct cggaaacttg
     5101 atgttaaagg cgctggatca ccttagcgtg cggcctctgg aagccatcaa tttaattcgt
     5161 aatctgctga aagtggatac gcatttgatt ccaatgtcag agcatcccgt cgatctgatg
     5221 gcgattgacg atcaggggca tgaagtttac ggcgaggtca atatcgacca gttaactacg
     5281 ccgattcaag agttattgtt aacgcctaat gtacccgcaa cgcgtgaggc ggttcacgct
     5341 atcaatgaag cggatctcat cattattggg cctggcagtt tttataccag cctgatgcca
     5401 attctgctgc tgaaggaaat cgcccaggca ttacgccgca cgccagcgcc gatggtttat
     5461 atcggcaatc tggggcgtga gttgagttta cctgcggcta atttaaagct ggaaagcaag
     5521 ctggcaatta tggagcagta tgttggtaaa aaggtcattg atgcggtcat cgtcgggcca
     5581 aaagtggatg tctcggcggt gaaagagcgg attgtgattc aggaggtact ggaggccagc
     5641 gatattccgt atcgtcatga ccgccagttg ttacataacg cgctggaaaa ggcgttacag
     5701 gctttaggtt aacggaaatc cgaagaaaaa ttccggcttc ctattgaaga caaagtgcgc
     5761 gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc gctaattcaa
     5821 tgtattgcag agatcatgta ggcctgataa gcgtagcaca tcaggctgtt ttccgtttgt
     5881 catcagtctt cttcgctatc ctgttacgat gcggcaataa acaactcacg caactgatgc
     5941 aactggtcac gaatttgtgc cgcttcttcg aactccagat tctgcgcgtg ttgcatcatc
     6001 agcccttcca gttcatggat tttctgctgc aacgctttcg gcgacatatc catcggcaca
     6061 ttatccggct caacaatcgg gcgcgatttt cctctgccct tcgctttggt tttggcaatg
     6121 ttctgcccca gcgccaggat atcgaccact ttcttgttca agccttgcgg cgtaatgccg
     6181 tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt cggtttcgcc aatcgctttc
     6241 gccattgatg gggtgatctt atcgccgtag agaatcgctt taccgttaac gttacgtgcc
     6301 gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc tttgtcagcg
     6361 tcgaggatcg ccaccagcga cacttccggc atatccagac cttcgcgcag taagttgatc
     6421 cctaccagca cgtcgaactc acccagacgc aagtcgcgga taatctccat acgttcgacg
     6481 gtgtcgatat ctgagtgaag ataacgcacg cgctcgccat gttcttcgag atattcggta
     6541 agatcttccg ccatccgctt ggtcagtgtg gtgaccagta cgcgttcgtt aattgccgct
     6601 cgctgacgaa tctccgaaag aagatcatca acctgtgtcg ccaccggccg cacttcgata
     6661 atcgggtcaa gcaatccggt tggacgcacc acctgatcca ccacatcgcc gccggatttt
     6721 tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg
     6781 aactcttcaa acttaagcgg acggttatcc agcgctgatg gcaggcggaa gccgtactcc
     6841 accagtgtct ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg tggaatggtg
     6901 acatgagatt catcgaccac cagcagccca tcggcaggga ggtaatcaaa cagcgtcggc
     6961 ggtggctcac ccggtccacg accggagagg aagcgcgagt agttttcaat ccccgaacag
     7021 tagcccagct cgttcatcat ctccagatca aactgggtac gctgggtcag ccgctgctct
     7081 tccagcagtt tgttgttttc caacagcact ttgcgtctgg cggccagctc ttctttgatc
     7141 tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt agtgcgtttt cgggtagatg
     7201 gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg ggtcaaataa cgacaatcgt
     7261 tccacttcct cgtcaaacag ttccacgcga agtgcaatgt catccgattc tgccgggaag
     7321 atatctatca cctcgccacg aacgcggaaa gtaccacgct ggaatgcttg atcattacga
     7381 gcgtattgca gctccgccag tcggcgcaga atcgcgcgct gatcgataat cataccgacc
     7441 gtgagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata aatcgcggaa
     7501 acagacgcca ccacaaccac atcacgccgc tccagcatcg ctttggtggc ggacaaacgc
     7561 atctgctcaa tatgttcgtt aaccgaggca tctttctcaa tgaaagtgtc ggaactcggt
     7621 acataggctt ccggctgata gtagtcgtag taggaaacga aatattccac cgcgttttcc
     7681 gggaagaact ctttcatttc gccatacagc tgggccgcca gcgttttgtt gggcgcaagt
     7741 accatggttg ggcgctgaag gtcagcaatg acattggcaa tggtgaatgt tttcccggag
     7801 ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt ccagcccctc ttcgagacgt
     7861 cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg aattcagttt gaacggttta
     7921 ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac tcgtcgtact
     7981 taattatgcc aacaaattat actggataaa aaaacagttc atcaccataa tatttctgtt
     8041 acagcgtaaa ctccgctgtc aatcatgagc aaaatttact ctgtggcgag ataaaactcc
     8101 ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag atgagtgatg
     8161 acagttttat ggcaagagat gcctgttcag tgactgccat tgcatttatc taaccagtta
     8221 aaaaataaaa gatatttctt tgagccgtct ttaacgccaa ttcgcgtgca agccgcgtat
     8281 tctctcgctt gcctcgtgtt ttctaactct aatacacatg gttatccaca ggaatagtgg
     8341 ataactgtct ccagccccta tcccccgccg cctgggcaat tcccacattc gccgcatgag
     8401 gcggaagtaa aatttttttc gtgattattt tagaatttaa ttggttaaat tgcagtcaat
     8461 cgaagacgcg atctcgctcg caatttaacc aaatacagga tagctacaac aaggcaaggt
     8521 ttatgtactt tccggttgcc gcattttctg gattttctgc aagccagggg atctctccca
     8581 gcagcggcgc gggaatcatg cgggtgagcg tggtcatata ttcagcgtga cgttttcccg
     8641 gaggcgtaac atcgttcgcc acccaacccg ccagagtcag tccggcgtgt tgtattgcct
     8701 gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac accaactacc agtatcaccg
     8761 gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt gtcagaaagc ggcgtaaacc
     8821 agccgccagc accttccact aacacccagt cagcctgttg ttcaagcgcg cgtaatccgg
     8881 cgctcattac caatgattct atcggcctgc cctcttgcgc gctgatgatg tgcggcgagg
     8941 tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc cagctgcagg ctgctgttgt
     9001 gctgtaacgc cagcgcgtca ctattacgta agccttccgg ggtcttttcg ctgccagagg
     9061 cgaccggttt ataacctgcc gtccgataac ctactgcctt cgcggcttgt aaaagtgcac
     9121 aactggcgac ggttttcccc acttcggtat ccgttccggt gacaaaataa cgtttactca
     9181 cgagcaatca ctcccaaaaa aagatgatac gtcagaggat atcgcccctg ttgttgcggc
     9241 caggccagtt gcaatcgctg caattgcgaa cgcgttaata ttcgcgggtc gcgcccttca
     9301 tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg cactgagcgc atcatcaaac
     9361 catagtgtga tgggctgaat atgatgttga taatgcacgc cgttcagcga ctgttcgatt
     9421 tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt ccactgcctg ccacgcctga
     9481 tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg cgaccacgcc tccggggcgc
     9541 accacccgat acagctcgcg gagtgccgtg gataaattac cgcaccactg caccgcgaga
     9601 ttgctccatg caagatcgaa cgtcgcagtc gctaacggca gggattcgat atctcccgcc
     9661 agataatggt ctgcggcatc cttctggcgt gcctgaacaa gcattggcgg cgagagatct
     9721 aacgccgtca cctgcgcgtg acgctctcgc cagtggcggc tcatccagcc tggcccacac
     9781 cccgcgtcca gtacgtgggt gtatttacgc tgtgggagca ttgccagtaa gacgtcagca
     9841 ctctggcgct gtagatctgc gtgttgctca tagtgcgcgg ctgcccgacc aaatgccgct
     9901 gcaatggctt gtttattaac cgttgccatg cagcatctcc agcaaacggt caatatcctg
     9961 catttcatgg gcggcggtta aggtcaggcg tagccgcgcg gtgccagcgg gtacggttgg
    10021 cgggcgaatc gccgtcaccc agcaaccttg ctggcgcagt ttttctgcca gctgtaacgc
    10081 acggctgtta tcaccgacga tcaatggctg gatggcgcta cgtgaatccg caagcgtaaa
    10141 cggcgaaccc tgcactccgg cacgaaaacg cgtaatgagt gccgccagtt tttcgcgccg
    10201 agcatccccc tcttcactgc gaatgaccgc cagcgacgca cgtaatgcct gcgcctgagc
    10261 gggcggcata ctggtgctgt agataagatg gcgggcgaat tgcagcagat aatccgccac
    10321 cgtactggag caaagcaccg ctgccccgct gacgccaaat cctttgccaa aagtcactac
    10381 cagcaattct ggttttacct tttgcagcca gcagctgccg cgcccctgct ccccgataac
    10441 gcccgtgccg tgggcatcat cgaccatcaa ccagccattg tgctgttgcg ttacctgctg
    10501 gatttccgcc agtggcgcac tatcgccgtc catgctgaac acgccttctg tcaccaccag
    10561 ttgctgcccc ggacaggagg cagcaagcag gcgcgccaga tgagtaacat cgttatgagc
    10621 aaaacggcga agctgcgccg ggcttaaact ggcagcctcc agcaaggagg cgtggctaag
    10681 ccggtcggca acaatacggt cctcttttgc catcatcgcg gtgatgaccg cctgattagc
    10741 ggcaaaaccg gagataaaca gcaatgcccg cgaatagccc agccactcgg ccagttcttc
    10801 ttccagcgcc tgatgcgcca cgctataacc gctgacatgc ccggagccgc cgctaccgac
    10861 gccaaattgt tccgccccct gctgccaggc gcggatgatg tgcggatgat ggcttaagcc
    10921 taagtaatcg ttactggaaa agttcagata ctggcgatcg tccgccacca gccagcgtcc
    10981 ggctccttgt gttaccggat aacggcgacg cagggcatcg gtagtacgcc gcgcagcgag
    11041 cgccgcgttg attttctcct gccagctcat agtgctgccg cgttgtaata ttcgtcagtg
    11101 tccggggtca tcagcgcctg ctccagacgc tgctgttgtt cgttatcccc tgccagcacg
    11161 gcagtttgct gcggatttag ccccagtttg cggaacagtt gcaggtcttt atcttcttcc
    11221 ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg agtttgcgcc tgccataaag
    11281 cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg cagaaaggcg cacgtaagag
    11341 gtcggcatca tgatccgcgc gaccgcaatg gtgcgaataa aatcaaaggc atcaacgtca
    11401 tcgttatcgg caagcggcgt gcctttcacc ttcaccagca tgttgattgg cacgctttcc
    11461 ggcggcgtcg gcaggtttgc cagttgccgc aataatccgg cgcgatcttt taccgtttcg
    11521 cctaagccca caatgccgcc agaacagact ttgatcccgg catcgcgcac tttttccagc
    11581 gtatcgaggc gttcctgata agtgcgggtg gtgatgatat tgccgtaaaa ctccggcgag
    11641 gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg cgaggcgctg cgcctgagat
    11701 tcactcaacg tgcccagcgt catacacgcc tccagcccca tcgcttttac cccctgcacc
    11761 atttgttcca ggtacggcat atcgcgttcg tggggattct tccacgccgc gcccatacag
    11821 aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact ccagcacctg ttcaacttcc
    11881 atcaaccgct cggcttccag cccggttttg tagcgcgagc tttgcgggca gtatttgcaa
    11941 tcttccggac aagctccggt cttaatcgac agcaacgtgc tgacctgcac ctgacgagga
    12001 tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca gatccagcaa cggtttttca
    12061 aataattctg tgacttgcga caatgtccag cgtgggcggt gagccatggg gcttctccaa
    12121 aacgtgtttt ttgttgttaa ttcggtgtag acttgtaaac ctaaatcttt tcaatttggt
    12181 ttacaagtcg attatgacaa cggacgatct tgcctttgac caacgccata tctggcaccc
    12241 atacacatcc atgacctccc ctctaccggt ttatccggtg gcgagcgccg aaggttgcga
    12301 gctaattttg tctgacggca gacgcctggt tgacggtatg tcgtcctggt gggcggcgat
    12361 ccacggctac aatcacccac agcttaatgc ggcgatgaag tcgcaaattg atgccatgtc
    12421 gcatgtgatg tttggcggta tcacccatac gccagccatt gagctgtgcc gcaaactggt
    12481 ggcgatgacg ccgcaaccgc tggagtgcgt ttttcttgcg gactccggtt ccgtagcggt
    12541 ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc aaaggcgaag cgcgccagcg
    12601 ttttctgacc ttccgcaatg gttatcatgg cgataccttt ggcgcgatgt cggtgtgcga
    12661 tccggataac tcaatgcaca gtctgtggaa aggctacctg ccagaaaacc tgtttgctcc
    12721 cgccccgcaa agccgcatgg atggcgaatg ggatgagcgc gatatggtgg gctttgcccg
    12781 cctgatggcg gcgcatcgtc atgaaatcgc ggcggtgatc attgagccga ttgtccaggg
    12841 cgcaggcggg atgcgcatgt accatccgga atggttaaaa cgaatccgca aaatgtgcga
    12901 tcgcgaaggt atcttgctga ttgccgacga gatcgccacc ggatttggtc gtaccggcaa
    12961 actgtttgcc tgtgaatatg cagaaatcgc gccggacatt ttgtgcctcg gtaaagcctt
    13021 aaccggcggc acaatgaccc tttccgccac actcaccacg cgcgaggttg cagaaaccat
    13081 cagtaacggc gaagccggct gctttatgca cgggccaact tttatgggca atccgctggc
    13141 ctgcgcggct gcaaacgcca gcctggcgat tctcgaatct ggcgactggc agcaacaggt
    13201 ggcggatatt gaagtacagc tgcgcgagca acttgccccc gcccgtaatg ccgaaatggt
    13261 tgccgatgtg cgcgtgctgg gggcaatcgg cgtagtcgaa acaactcgtc cggtgaatat
    13321 ggcggcgctg caaaaattct ttgtcgaaca gggtgtctgg atccggcctt ttggcaaact
    13381 gatttacctg atgccgccct atattattct cccgcaacag ttgcagcgtc tgaccgcagc
    13441 ggttaaccgt gcggtgcagg atgaaacatt tttttgccaa taacgggaag tcagcgtgtg
    13501 ggtttctggc tacactttct gcaaactaga aaggagggtt catgaaactc atcagtaatg
    13561 atctgcgcga tggcgataag ttgccgcatc gtcatgtctt taacggcatg ggttacgatg
    13621 gcgataatat ttcaccgcat ctggcgtggg atgatgttcc tgcgggaacg aaaagttttg
    13681 tcgtcacctg ctatgacccg gatgcgccaa ccggctccgg ctggtggcac tgggtagttg
    13741 ttaacttacc cgctgatacc cgcgtattac cgcaagggtt tggctctggt ctggtagcaa
    13801 tgccagacgg cgttttgcag acgcgtaccg actttggtaa aaccgggtac gatggcgcag
    13861 caccgccgaa aggcgaaact catcgctaca tttttaccgt tcacgcgctg gatatagaac
    13921 gtattgatgt cgatgaaggt gccagcggcg cgatggtcgg gtttaacgtt catttccact
    13981 ctctggcaag cgcctcgatt actgcgatgt ttagttaatc actctgccag atggcgcaat
    14041 gccatctggt atcacttaaa ggtattaaaa acaacttttt gtctttttac cttcccgttt
    14101 cgctcaagtt agtataaaaa agcaggcttc aacggattca tttttctatt tcatagcccg
    14161 gagcaacctg tgaacacatt ttcagtttcc cgtctggcgc tggcattggc ttttggcgtg
    14221 acgctgaccg cctgtagctc aaccccgccc gatcaacgtc cttctgatca aaccgcgcct
    14281 ggtacctctt ctcgcccgat tctgtcggca aaagaagcgc agaatttcga tgctcaacac
    14341 tattttgcat ccctgacacc aggtgctgca gcgtggaatc cttccccgat taccctgcct
    14401 gcgcaacctg actttgttgt cggcccggcg ggcactcaag gtgtaacgca taccacgatt
    14461 caggcggcgg tagatgcggc aattatcaag cgtaccaaca agcgccagta tattgccgtg
    14521 atgcctggtg agtatcaggg aacggtatat gtccctgccg ctccgggtgg aattactctg
    14581 tacggtacag gtgaaaaacc gattgatgtg aagattgggc tttcccttga tggtggcatg
    14641 agccctgccg actggcgtca cgacgtcaac ccgcgcggca aatatatgcc aggtaaacca
    14701 gcgtggtata tgtacgatag ctgccagagc aaacgcagcg acagtatcgg tgttctctgc
    14761 tctgcggtct tctggtcaca aaacaatggc ctgcaactgc aaaatctgac catcgaaaac
    14821 acgctgggcg atagcgtaga tgcaggtaac catccggcgg tggcactgcg tactgatggt
    14881 gaccaggtac agattaacaa cgttaacatt ctcggtcgtc agaacacctt ctttgtcacc
    14941 aacagcggtg tgcagaaccg tctggaaacc aaccgtcagc cgcgtacgct ggtgaccaac
    15001 agctacattg aaggggatgt ggatatcgtt tctggtcgcg gcgcagtggt gttcgataac
    15061 accgaattcc gcgtggtgaa ctcacgtact cagcaagaag cgtatgtgtt tgcaccggct
    15121 acgctgtcca acatttacta cggtttcctc gccgtaaaca gccgtttcaa tgctttcggt
    15181 gatggtgtgg cgcaactggg ccgctcgctg gatgttgatg ccaataccaa cggtcaggtg
    15241 gtgatccgtg atagcgccat caacgaaggt tttaacacgg ctaaaccgtg ggccgatgcg
    15301 gtgatctcta atcgtccgtt tgcgggtaat accggcagcg tagatgataa cgacgaaata
    15361 cagcgcaatc tgaatgacac taactacaac cgcatgtggg aatacaataa ccgcggcgtg
    15421 ggtagtaaag tggttgcaga ggcgaagaag taagagcaat taactatttg ccggatgcgg
    15481 cgtaaacgcc ttatccggcc tacggttcga tgcgatttgt aggtcggata agatgcgcaa
    15541 gcatcgcatc cgacaataag tgccggatgc tgcgaaaatg ccttatctgg cctacagatt
    15601 cgatgcgatt cgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgaa
    15661 tgcgacctac attcacatgg cgctttttac atctgacggt ttttattgaa gttaatcaaa
    15721 ctacccgcct tgataatctc gcgctcttca gcagtcagac tttccatata gagcgtaatt
    15781 tccgttaccg gcgcatcttc atggatcaca taacctttaa acgtcgtacc cggattatcc
    15841 agcgccgctt taatgccagg gatgtaaatg taatccccca cttcaaaggt tggcacttcc
    15901 gccatctgca gcggtaacat cccccagttg atgacgttag aacggtagcg tttagtcgcg
    15961 tactcttcgg caatattcgc cagaccgcca atcacacgct ggcagctcgc cgcctgttca
    16021 cgcgcagaac catcgcctgg tttcaccgca tataccatgc tgccaatttc agtttgcagc
    16081 ggatcaatat gctcctgacc agcaatctgc ttaatgcgcg caaacacctc tgtcagctcg
    16141 ctgacattcc ccgccagacg ctgattttcc agctcagcag tcgctttact tctgccaaca
    16201 taaccgggat cgcggcgtga cagggtaaac tccgccagac caatcggatt tgaacgataa
    16261 gaagaggttt caccggaagg aatcagttcg tcggtggtgg tcacttcgtc gaggatcttc
    16321 gagcacactt tcaggacgat attgtcagtc agcgcaccca attccggcca gtctttaatg
    16381 ttcggtccgt aaatcagcgg ttgctgagtt gcccctttca caaagccctg ataaacacgg
    16441 tttttatacg gcgttacatc gaaggcgtac tccggcacgt tgtcccagca atcaagttcg
    16501 ctggcagagg ttaaatagcc accgtttgcc gcagtcgcag cgatagaacg agcgtccatc
    16561 aacgccaccg ctgacatctg cccattagct ggcttagagc cttcgcggtt cgggaagtta
    16621 cgcgtggtgt ggcgaatact taatccgttg ttgattggcg tatcgcccgc gccaaagcat
    16681 gggccgcaga acgcggttct gatgattgcg cctgcgccaa tcaaatctgc taccacacct
    16741 tttttggcga gatccataaa caccggctgt gatgacgggt aaactgccag cgagaaggtg
    16801 tcattgccac aggattgacc gcgcagtgca ttcgccgccg cgatgacgtt ttcgtagtta
    16861 ccgccagaac agcccgcgat aatcccctgc tgcactttca ggcgaccatt ttccacttta
    16921 tccagcagcg agagtttggc tttaccgtgc gccacgcgtt cggactcaat ttcaatctca
    16981 cgcagaatgt cggtcaggtt ctggttcagt gtgtcgattt catacacgtt gctcgggtgg
    17041 aacggcagcg caatcattgg tttgatggcg cttaaatcaa cgctgatgca gccatcgtag
    17101 tacgccatcg gttgagggtt aagctggcag taatcctggc cgcgaccgtg cagcgccagc
    17161 cagttatgca cttcttcatc agtttgccag acggagctta aacaggtggt ttccgtggtc
    17221 atcacgtcaa cgctgttacg gaaatcggta gagagcgcgc taacgcccgg tccaacgaac
    17281 tccatgactt tgtttttgac gtaaccgttt ttgaacaccg cgccaatgat agccagcgcc
    17341 acatcctgcg gccccacata cggcgctggt tttccggtca gatgcaccgc aacaactccc
    17401 ggatagtcga tatcccaggt gtcattaagc agctgtttta ccaactcacc accgccctca
    17461 ccgactgcca ttgtccctaa tgcaccgtaa cgggtgtggc tgtctgaccc gaggatcatt
    17521 ttgccgccgc ctgccatcat ctcacgcata tattgatgga tgaccgcaat atgcggaggc
    17581 acaaaaatac cgccataacg ctgagctgcc gataaaccaa aaacatggtc atcaccgtta
    17641 atggtgccgc cgacggcgca gagtgagtta tggcagttgg tcagcacata cggcagcggg
    17701 aaacgttcca taccggacgc tttagcggtc tgtacaatac caacaaaggt aatatcgtga
    17761 gaggctaatg aatcaaactt aattttaagt ttatccatat ttccggacgt attatgcgaa
    17821 gagagaatag accaggcaat agtgcctttt ttggcttctt cttttttaat ttcgccggtg
    17881 aaatgttctt cagcaattat ttcgttatta ctggcgagaa acacgccttt ttcagataac
    17941 ttgatcatca aatgctccag atatctgcgc accgtgaagt gcgcagaaag ataataaatg
    18001 gatatactct aaataatttg agttgcagga aggcgacaag cgagtgaatc cccaagagct
    18061 tacataagta agtgactggg gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg
    18121 acgagaatat ttacagcatg ttccagccga tcagcatatt ccaccaccac acgccgaggg
    18181 tgatatgcac caggaaggtt aatatcgtca gtaccgcacc gaccaaccac caggatttaa
    18241 tatcgttata acccacgcca aagatgaccg gacctgccgc gccgccatag tgagtaacca
    18301 tgccgccata ggagttggag aacaacagtg ccagcgcggt taacattaac ggtgcgccgg
    18361 agacgttcgc cagcatggca aataccggta acatagcaac gatataggca ctaccggaag
    18421 cgaagaaata acgcacgata atgctgagga aaataataac gaagaaagca acgttaccgt
    18481 gaccatcaaa tgccaggtta tttttaaaga cttcagctaa ccattcgaag aatttaactt
    18541 tcgataataa ggagcttaat ccgataatac cgccgtacca gattaaggta ttccagccgc
    18601 ctttattttt aaccacgtct tcccaggtaa cgatacccag cagcagcatg gtcgccataa
    18661 caacgattgc cacggtggat tcatcaaccc ccagagactt actgaaaatc cagcccagca
    18721 gcgccagcac aaagacaccg agcagcattt tttcgcggat tttcatcggt cccagttcgg
    18781 caaggcccgc tttagcgatg gttttattat ccaccttctt aatttctggc ggatacatgg
    18841 tataaatcac cagtggggtg accagcagca taatgatgcc cggcaatccg gcggccagtg
    18901 cccatccacc ccagctaatt tgcaggtgca gaatgtcgtt gatcattttc agcgccagaa
    18961 tgttccccgc cattgcggta aagaacatat agctggtggt tttggtgacc atgtaaatgg
    19021 acatcatcag gtaatgtccg acacgacgcg gacttttttc cggttcggac cccaaagcca
    19081 ccgccacgct gttgatgatc ggtaacacaa tgccgcccgc acgcgcggtg ttagacggtg
    19141 ttgccggagc cagtaccaga tcgaggaata ccgtaacgta acccagaccc agcgtggtgt
    19201 taccgatttt accaatcagc agataggcaa tacgtttacc taaaccggtg gtcacaaatg
    19261 cggcgcttaa ggtaaacgcc gagaacacca gccaggtagt acctgaagag taaccgctta
    19321 atacggcggt ggtcttaaac gcaccgtcgg ataagttacc gaccaccacc atcgaggcag
    19381 caacggcaat taacagtacg acaggttccg ggaaaggctt gataaccagc cccacaatgg
    19441 ccgccaggta aataccaaaa agcacccagg ccagttcgct taatcctgcc ggagctggca
    19501 taaagccaat aatacatggg atcgctaata tcagaattag cttccataac gatttcttat
    19561 tcatttaaaa tcctgttctt tctgtatgac tcatacagaa gtcctttatt gattgtcctt
    19621 atcctgacaa cgaatttttt cagggaagat aaacttcacc ggaaaatatt tttctggttg
    19681 tccgaataac agatgcgcgt aacgtcgtgg catcctgacc ttcattactt aaatgaacgt
    19741 cgagcgcacc actggggtgt tcaatattga tattgccgta tcctacagaa gggacgattt
    19801 gtcgggtgac ggtgccttcc aatgcacaac tactggaaat agcaatagca ccggttatcg
    19861 ccagcgcgcg atggcaagaa tgcggcataa aataacgcac attaattgcc ccgcctttct
    19921 gcgctggaga aataagcaca ggtttaggga taaccatatt actgacatca cctaagccca
    19981 ttgctttacc cgcttgtaga cggatagatt caatgcgggc taataatgct ttgtcggcat
    20041 ccagttccgc cggtaattca taacctgttt ttcccagata ttcagccgga ataatgacga
    20101 ctggcatcgc catatcgata caggtcaccg ggacatcgtc aaaataatca atctgattat
    20161 cagtcgggaa aacttttccg gttttggttc cagcggcatt caggaaagtg agcgcaaccg
    20221 gtgcggcagt acccggtacg ccgtcaattc tggcactacc ctcgtactcg acaacaccat
    20281 ttggcgtttg cacatcagct tcgatgaacg tacccgtatt gacgttgcgg atacgcacac
    20341 gggtgaccgg tgatgtcgct gcaatcaaac cattttcaat ggcgaatgcc ccaacgccag
    20401 aaagcatatt gccgcagtta ggcgtggtat cgacacgttg ctcatggacg ataacttgag
    20461 caaacagata atcaacatca gcacgcggat cgctggaacg gctaataatg gcgactttac
    20521 tggtcagtgg attaccgccg ccaataccgt caatttccag atcgttaccg gaacccataa
    20581 ttgccatcaa tattttatcg cgctgcgttt gatcttcggg taaatgttcc gctaacagga
    20641 acgcgcccct cgaggttcca cctcgcatca tcacgcaggg tatttttttc ataataatgc
    20701 cctcaattta aaaacataac ttattgcgat atggttacat taaggacaaa gcatctctaa
    20761 tgactttagc tcaacgatta ataacatttt ttcattaatt taagttggct aaatgcagtt
    20821 aaaattttta acgaccagcc acccaaaatc gtcgcttcca ttttccggag taataatgaa
    20881 gcatgaatta tcaagtatga aggcatttgt catactggca gagtccagtt catttaacaa
    20941 tgctgctaaa ttactcaata ttacgcaacc tgcattaacg cgaagaataa aaaagatgga
    21001 ggaagattta catattcaac tatttgagcg tacaactcgc aaggttacgt taacaaaagc
    21061 aggaaaaagg ttgctcccgg aggcgcggga attaataaag aaattcgatg aaacgctttt
    21121 taatattcgt gatatgaatg cttatcatcg tggtatggtg acgttagcgt gtattccgac
    21181 cgcagtattt tattttttac cgctggcaat cggtaaattt aatgagctgt atcccaatat
    21241 taaagtgcgg attctggaac aaggcacgaa taattgcatg gaatcggtgc tgtgtaacga
    21301 atcagacttt ggcataaaca tgaacaacgt cacaaattca tccattgatt ttaccccact
    21361 ggtcaacgag ccattcgtgc tggcctgtcg gcgtgaccat ccattggcca aaaagcagct
    21421 tgtagaatgg caggagttag tgggttacaa aatgattggc gtacgttcct ccagcggcaa
    21481 ccgattgttg atagaacaac agctagccga caagccctgg aagctggact ggttttacga
    21541 agtgcgtcat ctttcgacgt cattaggact ggtagaggcg ggactgggga tttcggcgct
    21601 tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt attggtattc cactggtgga
    21661 acccgttata cgtcggacat tggggattat tcgccgcaaa gatgccgtgc tttctccggc
    21721 agcagaacgc ttttttgcct tgctgattaa tttgtggact gacgataaag acaatttgtg
    21781 gaccaatatc gtcgaacgcc agagacatgc gttacaggag ataggttaat acaccggcgc
    21841 aggagagact gcgccggtta aatcagcggt tagtgtgcgt taaccaccac ccacattggt
    21901 ccctgcccga ccgcataacg gcctttttca tgcagtagtc cctgctcgcc aacaatttcg
    21961 tataccgaga tgtggtgaga tttttgcccg gcggcaatca gatacttgcc gctgtgatca
    22021 acattgaagc cgcgcggctg ggtttccgtt ggctggaagc cttctttact caacacgctg
    22081 ccatcttccg aaacgctgaa aacggtaatc aggctggcgg tacggtcgca ggcgtataaa
    22141 tggcgaccat ccggggtgat atgaatatca gccgcccaac gggtgtcgga gaagttttcc
    22201 ggcatcatat ccagcgtctg gacacattcg atattaccgt gcggatcttt cagttcccag
    22261 acatccactg agctgtttaa ctcattgacg caatacgcat attgttcgtt tggatggaat
    22321 accatatgac gcgggccggc cccttcaacg gtggtcactt ccgcagggtc ctgcgccacg
    22381 agatgaccat catcgctgac cgtaaacagg caaatgcgat cctgctttaa tgccggaacc
    22441 cacagcgtac ggttgtccgg tgagatattg gcggaatggc aaccgtccag cccctcgacc
    22501 acatcgacga cgcccactgg caggccatct tccagacgcg ttacgctcac gttacccgca
    22561 ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg tggaaatatg cgtcggacta
    22621 cccggcagcg cagactctgc ggcaaaggtc agtgcgccat cgtccggggc gatactatac
    22681 gccaggacgc gaaactcagg gcgaacacca acatagagat aacgtttgtc cgggctgacc
    22741 accatcggct gcacctgccc cggcacatcg acaacctgtg tcagcgtcag tgcgccttca
    22801 tgattcaggt tccagacgtg aatttgctgg ctctcagggc tggcgatata aactgtttgc
    22861 ttcatgaatg ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg
    22921 gcggtgaatg ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc
    22981 gcgcagacga aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct
    23041 ctcgacttag acggcacctt attgaccccg aaaaagaccc tgcttccttc atcgatagaa
    23101 gccctggccc gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac
    23161 gtcgctattc atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat
    23221 ggcacctatt tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt
    23281 aataaagccc tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat
    23341 gtggatgatg caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg
    23401 gcgcaaaccc tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa
    23461 acggcgcaac aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa
    23521 ttgcagcatt ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg
    23581 cacgatcagg ttgatattgc acgcggcggc aacagcaaag gtaaacgttt gacgaaatgg
    23641 gttgaggcgc aaggctggtc gatggaaaac gtcgtggcgt tcggcgataa ctttaatgat
    23701 atcagtatgc tggaggccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta
    23761 aaagcgcgcg ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt
    23821 tatagccacc tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca
    23881 caggccaggt ttgatcacca gttcatccct ggcccacggg ctgatacgcg cccacagcgt
    23941 tttacctccg acttccagtt ccacttccac ctggccgttg tcgtcataac tattaacaac
    24001 ttttgcccgc aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac
    24061 atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc ggttcgtcca gcttattgac
    24121 ccacaaatgc tgatcgccca gcgccagcgc agtcatcgcg taatgcggat gatgctcaag
    24181 taccgtcact ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct
    24241 actaccccag acttcttcca gtgcgccaaa ggctttcacc tgaccgtttt ccagtaccat
    24301 cactctgtct gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcgggat
    24361 gttgatttcc cgcgtcagac gttgcagata aggcaacagt tcgcgtttac gcggaatatc
    24421 cagtgacgcc agcggttcat ccagcaacag caattctggt gccgtcagca aagcccgccc
    24481 aatcgctacg cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg
    24541 ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg accatgcttt tcgccatgcc
    24601 gtagcgcaga ttgccacgca ctttgtaatg cggaaacagc cgcgcatcct gaaaaacata
    24661 gccaacgcga cgcttttctg gcgtcaggca gatacctttt tcggcatcat ttagtacccg
    24721 accattgagg acaatcctcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag
    24781 cgaagttttt ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt
    24841 ttcattaatg gtcaggcaat ggttgcccaa ggtctgggaa aaattcagtt ccagcatgat
    24901 tagcgccccg cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc
    24961 atcgccagcg caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc
    25021 tggatcaggg tatacatggc agaaggaatg gttcgcgttt caccaggaat gttcgacaca
    25081 aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca
    25141 ataattcccg gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggcc
    25201 cccagtgttc ttgcggcctg ttcgagtttg acatcaaccc cttccagcgc cagacgaatt
    25261 gcccgcacca tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta
    25321 aaggcgaagg taatgccaaa ccagtcatac agacgttcac cgataaaccc gcgccgtccc
    25381 atcgaaacta ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt
    25441 acgctgtcga gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag
    25501 atcccaaacg gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact
    25561 gcctgccatt ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga
    25621 tttccgcagc ctgcggccct ttcaaataat cataaaatgc tttcactgtc gcattgttat
    25681 gcccttccac aacagcaacc gggtattcca cttttttatg cgaatcttcc gggaaggtgg
    25741 cgaccacttt tacccctttg ctggcaactg cgtcagaacc gtaaactatg cccagcggcg
    25801 cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc ttccgccgga gccagtttcg
    25861 gagagagcgt atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg
    25921 gaacatgttc cggatcgcca accgccagac gaccgccatt cagcagcgaa gtccagttgg
    25981 ttttgctgtc gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc
    26041 tattgccgag cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat
    26101 ccatccattt ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac
    26161 gggcgagagt agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat
    26221 actgcgtagc aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt
    26281 tcccttcatc tgccagtgca ttgccagcaa cagcgaaaga gagtgccgcc ccggcaaaca
    26341 agttcaacca tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata
    26401 taacgactaa ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa
    26461 aaaatatcgg caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcat
    26521 tcagggaatc aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg
    26581 aatacttcac caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg
    26641 attacggcga ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat
    26701 gagactattc taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg
    26761 tggcgttcgt tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat
    26821 ccttctcacc cttaagctcc aacaaaaatt attcgccgac ccgcgccgga tttcgctact
    26881 aaaacacatt gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta
    26941 taaaagcgcc tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga
    27001 gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg acccgctatg gtcagcgact
    27061 gattcagctc tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga
    27121 cgatgacgcc ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac
    27181 cagcgcccgt aaccagtggt tcggcaccat caccgcccgc gatcatgatg acgttcaaca
    27241 gcatgttgat gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca
    27301 aagcggcgcg cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc
    27361 gtgggtaggt attactcagg acgaggcggt cgcgcaaaac gctgacaacc aattaccggg
    27421 tattattagt catattgagc acggcgcaga gcagtgcgaa gtattaatgg cgctacccga
    27481 cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca
    27541 gaatgtcacg gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg
    27601 ttgacaattt gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata
    27661 aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc
    27721 aattgcctca gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa
    27781 gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac
    27841 ggcaaagcca gttttcccac atcactcgtc tctccttcga gcaattgcaa aaactcgtca
    27901 gcgacgaatg gcagcggaat aacaccgata tgctcggccc tggcgaagat gacaccggac
    27961 gcactacggc tgagatcatt caggatgaag taaaggatgc accgcgttgc atgcaactgg
    28021 cgcagcagtt cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg
    28081 agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg
    28141 atgagccgtt cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct
    28201 cgttacatca gtccggtatt actctggtac tggtgctcaa tcgcttcgat gagatcccgg
    28261 agtttgtcca gtttgctggc gtgctggcgg attgcacgtt agcggaaact ggcgctaaag
    28321 aggaactgct ccaacaagca ctcgtcgcgc aactggcgca tagtgaacag cttgaaggtg
    28381 tgcaactgcc ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaaccgc
    28441 gcattgtgct gaacaatggc gtggtttctt ataacgatcg ccccattctt aataacctta
    28501 gctggcaggt gaatccaggc gaacactggc aaattgtcgg gccaaatggt gcaggaaaat
    28561 cgacgttatt aagcctggtt actggcgatc atccgcaagg ttacagcaac gatttgacgc
    28621 ttttcggacg acgtcgcggc agcggcgaaa ccatctggga tatcaaaaag catatcggtt
    28681 acgtcagcag tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc
    28741 tttctggcta ttttgattcg attggcattt atcaggccgt ttcggatcgc cagcaaaaac
    28801 tggtgcagca gtggctggat attctcggca ttgataaacg tacggctgac gctccgttcc
    28861 atagtctttc ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc
    28921 cgacgttgct tattctcgat gaaccactac aggggcttga tccgctcaat cgccagctta
    28981 tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc
    29041 accacgctga agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg
    29101 gactctatcg ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag
    29161 gcggcctaag cgccctctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt
    29221 ttttcagata aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc
    29281 ataaaaaacg gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt
    29341 tcgcatcttt gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag
    29401 agttctggtt accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca
    29461 aaacggtcat gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc
    29521 tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga
    29581 agcgttgatg accgagatac tgcacgatca cgctatcgac accgtgatcc acttcgccgg
    29641 gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa
    29701 cggcactctg cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag
    29761 ctcctccgct accgtttatg gcgatcagcc caaaattcca tacgttgaaa gcttcccgac
    29821 cggcacaccg caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga
    29881 cctgcaaaaa gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg
    29941 cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc
    30001 atacatcgcc caggttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta
    30061 tccgaccgaa gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg
    30121 tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg
    30181 tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa
    30241 accggttaac tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga
    30301 cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc
    30361 gcaggacacc tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac
    30421 catgacgcaa tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca
    30481 atggattctg gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc
    30541 agccaaacag gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg
    30601 ggtgacaggc gataaaaacc ccgattacac cagaacttac gttttcacta atgactttgc
    30661 ggccttgatg tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca
    30721 gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc
    30781 agaactgagc gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga
    30841 gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg
    30901 ctctaacccg catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga
    30961 gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga
    31021 ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt
    31081 agccgttgtg ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca
    31141 cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa
    31201 aaagctgacc agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg
    31261 gcacggcgca ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta
    31321 tccgcctctg ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc
    31381 agagacccag cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga
    31441 tatccatttt cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct
    31501 gtttgccaac gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa
    31561 tttgattggt gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta
    31621 tcaaaccgtg atcagctgtg cgccacacga tgaccgtaaa gttcgcgtaa tggcagccga
    31681 ttatgaaaat cagctcgacg agttttccct cgatgcgccc attgtcgcgc atgaaaacta
    31741 tcaatgggcg aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt
    31801 cggcggcgtg gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc
    31861 cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga
    31921 cggcgcacaa atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg
    31981 cgggatcatg gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga
    32041 ctgccgctca ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat
    32101 caacagtaac ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg
    32161 cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt
    32221 caacgctgtt gcacatgagc tggacccaat cgtggcgaaa cgcgtgcggc atatcctgac
    32281 tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat
    32341 gggcgagtta atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc
    32401 gcaaattgac actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg
    32461 catgaccggc ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc
    32521 tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt
    32581 ttacgtttgt aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca
    32641 cccgatggtc agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg
    32701 ctgatggact ggggtgcgac tttactttca gctcgtattc cgctttccga tggcagcgtc
    32761 cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc aggatcaggc tgcgtttctg
    32821 ggggcctcta ttggtcgtta tgccaaccgc atcgccaata gccgttatac ctttgacggt
    32881 gaaaccgtga cgctttcgcc aagtcagggc gttaaccagc tgcacggcgg cccggaaggg
    32941 ttcgacaaac gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg
    33001 agttcagatg atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt
    33061 ctgaccgacg ataaccgtat ctctattact tatcgcgcca cagttgataa accttgcccg
    33121 gtgaatatga ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat
    33181 cacaagttgc agattctggc ggacgaatat ctgccggttg atgaaggcgg cattccgcac
    33241 gacggcctga aatctgtcgc cagaacgtct tttgatttcc gtagcgccaa aatcatcgcc
    33301 agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg
    33361 ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa
    33421 ttgcagctga aggtctacac caccgctccg gctctgcaat tctactccgg caacttcctc
    33481 ggcggcacgc catcaagagg aactgaacct tacgccgact ggcaaggcct ggcactggaa
    33541 agcgagtttc tgccggacag cccaaaccac cctgaatggc cgcaaccgga ctgcttcctg
    33601 cgtcctggcg aagagtattc cagcctgacg gaatatcagt ttattgctga gtagcacctg
    33661 aagccctcgt aatgagggct ttatccttat attgctcaaa tcaccagcaa acaccgacat
    33721 atttgcaact caatattcac aacaacctta cactgcgaca ctattttcgc tatggttatg
    33781 cgtaagcatt gctgttgctt cgtcacggca atataatgag aattattatc attaaaaaat
    33841 gatttgagga gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt
    33901 cagtggaaca aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc
    33961 gtaagcgaag caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt
    34021 gcttacacct ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg
    34081 gatcaggcat ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg
    34141 ttgcagggtc tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag
    34201 tggcgtcgtg gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg
    34261 ggtcacgatc cgcgttacgc gaaactgagc gaaaaagaac tgccgctgac ggaaagcctg
    34321 gcgctgacca ttgaccgcgt gatcccttac tggaatgaaa ctattctgcc gcgtatgaag
    34381 agcggtgagc gcgtgatcat cgctgcacac ggtaactctt tacgtgcgct ggtgaaatat
    34441 cttgataaca tgagcgaaga agagattctt gagcttaata tcccgactgg cgtgccgctg
    34501 gtgtatgagt tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac
    34561 gagatcgcag cgaaagcagc ggcggttgca aaccagggta aagcgaagta aacgtcattc
    34621 gtttaaaatg agaaagccga ctgcaagtga gtcggcattt ttgttgctaa caatggagca
    34681 cattgcctga tgcgacgctg cgcgacttat caggcctgtg gtgattcatc ggatacgcca
    34741 ctctgacggc gcatccgaca attaaacctt acccgcgacg cgcttttact gcattcgcca
    34801 gttgacgtaa cagagcatcg gtatcttccc agccgatgca ggcatcggtg atgctcttac
    34861 cgtaggccag cggctccccg ctctcgaggc tctgattgcc ttccaccaga tggctttcca
    34921 ccatcacgcc aataatggcc ttttcgccac cggcaatctg ctggcaaacg tcagcacaaa
    34981 catccatctg ctttttgaat tgtttggacg agttagcatg gctgaaatcg atcatcacct
    35041 gtgctggcag gcctgctttg ttcagccctt ctttcacttc agcaacgtgc ttcgcgctgt
    35101 agttaggctc tttaccgccg cgcagaatga tatggcaatc gccgttaccg ctggtattca
    35161 caatcgccga atgcccccat ttcgttacgg acaggaagca gtgcggcgca ccggcggcat
    35221 taatggcatc gatagccact ttaatcgtac cgtcggtgcc atttttgaag ccgaccggac
    35281 aagaaagccc tgatgccagt tcgcggtgca cctgcgattc ggtggtacgt gcgccaattg
    35341 cgccccagct catcaggtca gcgagatatt gtggggtgat catatcgaga aactcacctg
    35401 ccgctggcag accgctgtcg ttaatatcaa gcagcaattt acgggctata cgcagaccgt
    35461 cgttgatctg gaagctatta tccatatgcg gatcgttaat cagccctttc cagcccaccg
    35521 tggtacgcgg cttttcaaaa tagacgcgca ttacgatttc cagctcatct ttcagctctt
    35581 cacgcagcgc cagcaagcga gtggcatact cttttgccgc gacaggatca tgaattgagc
    35641 atgggccaat cacaaccaac aggcgatcat cattaccttt caggatctta tggatcgctt
    35701 ttcgggcatg ggcaaccgta ttcgcggcat tttcagtagc ggggaatttt tccagcaatg
    35761 cgacaggagg aagtaactct ttgatttctt tgatgcgtaa atcgtcgttc tgataattca
    35821 tgtctgttcc agtgttgcca tacttatctt agtgaatgca atacttccaa tctatatctt
    35881 ccgtcagaat gtgtaaacgg ggttttacac tatgaacgga ttaatcctgg aattagcaag
    35941 aaaaacgcca gattgtcgcg aaaaacgaga tctctcctac aatttctaac tgtaactcct
    36001 ttgtttgtta attatttcaa gattctctgc tgcgtttcat aacctggctg aaatcttaaa
    36061 ccaatgcctt atattcacct gcaaatgcac tgttggaaga ggttatccga cataacgacc
    36121 ataacaggag catcctatga aaatgacaaa actggccaca ttatttctga ctgccactct
    36181 aagccttgcc agcggtgccg cactggctgc cgatagcgga gcgcaaacta ataacggcca
    36241 ggcaaacgcc gcagctgatg cgggccaggt agcccctgac gcccgtgaaa atgtcgcgcc
    36301 aaataacgtc gacaataacg gggtaaatac cggttctggt ggcacaatgc tgcattcgga
    36361 tggttcttca atgaacaatg acggaatgac caaagatgaa gagcacaaga acaccatgtg
    36421 caaagatggt cgctgtccgg atatcaataa aaaggtacaa accggtgatg gcatcaataa
    36481 tgatgtcgat accaaaaccg acggtaccac gcagtaaccc tgcaaaatag ccaataatgg
    36541 gagagcggag tctctccctt ttaatttttc acttctaaaa gcagtatgat gtcataaagt
    36601 taagtgagaa taattaaggg atgactcgtc atggcgcact cacactcaca cacgtcctca
    36661 cacctgccag aagataataa tgctcgtcgc ttgttgtatg ctttcggcgt gactgccggg
    36721 tttatgctgg tagaagtcgt tggtggtttt ctttctggtt ctctggcatt gctggccgat
    36781 gcgggtcata tgttgaccga tactgccgcc ctgcttttcg ccctactcgc cgtgcaattt
    36841 tcacgtcgtc ctcccaccat tcgccacact ttcggttggc taagactgac cacgctcgcg
    36901 gcgtttgtga atgccatagc cctcgtggta attaccattt tgattgtctg ggaggcgata
    36961 gaacgcttcc gcacgccgcg tccggtcgag ggcggcatga tgatggcaat tgccgtggct
    37021 gggttgctgg caaatatact ttctttctgg ttacttcatc acggcagcga agagaaaaac
    37081 ctcaacgtac gagcagcggc actgcatgtg ctcggggacc tgctgggttc ggttggggct
    37141 attattgccg cgttgattat tatctggact ggctggacac ctgctgaccc cattctctcg
    37201 atactggtgt cgcttctggt tctgcgtagc gcgtggcgac tgttgaaaga tagcgtgaat
    37261 gaattacttg aaggtgcacc ggtatcgctg gatatcgctg aactgaagcg tcgtatgtgc
    37321 cgggaaatcc cggaagtccg caatgtgcac catgtacatg tatggatggt aggcgagaag
    37381 ccggtgatga cgttgcatgt gcaggtgatc ccgccacacg aacacgatgc cttgttggat
    37441 cagatccaac actacctgat ggatcactat cagattgagc acgccaccat tcagatggaa
    37501 tatcaacctt gtcatgggcc agactgccac ctgaatgaag gggtgtctgg ccattcacat
    37561 catcaccatt aatgggacag cgcgcgtgag cctctttcac gtgcgctgtt gatccacatc
    37621 cggctgccgt tgagcgcaat aaaggtcagg atgatgtact ccagagacat ggcgtaaacg
    37681 ccctgaagtg caaaaataac gacgctaatc acgttaataa tcacccacaa cagccagttt
    37741 tccacatact tacgcgtcat cagaatcatt gccacgatag ataacaccat catgcatgaa
    37801 tcccagaacg ggaaagcgtc cggttgcagt tcaggcatca caacctgtaa tcctaatgct
    37861 tgcatgatca tgactgccac gcgggtcaaa aatgcaaaca ccggattgat aaataccgtc
    37921 atcagaccaa tcgaaacaac gcaaaccgcc aaccagctga gtgccttcgg caatggcaac
    37981 cagcgaattt tcaactccgc ctcgttctga ctggtttgtc gcgaccacgc ataccaaccg
    38041 taaatattcg cggcaaagaa aaacacctgt aatagcaggc tggcatacag ctgaatctga
    38101 aagaaaataa tgccaaacaa ggtgacgttg atcaggccaa agaagtagtt gctgatcttc
    38161 tccagactgg caaggccaat acacagcaac ccggcgatcg tgcctaccgc ttcgatccat
    38221 gagagatcat aaccgcctgc ccctattggt atatgtacca ggatattctg cacactaaaa
    38281 aaatccatct ttcccccaaa gcgtaacgtt acgccttatt attcgttatc cacgtagtgt
    38341 agccgcaaaa tccagcatac gattgagcgg caccagcgcc ctctctcgca gcctttcatc
    38401 aacatgaacc tcgtgattgc ttccttcctg ttctaatgcc tctgcgatgg actgaaggcc
    38461 attcatggcc atccacggac aatgcgcgca gctgcggcag gttgcaccct cacctgcggt
    38521 tggtgcttcc agtaactctt tatctggcac tgcctgctgc attttgtaga aaatgccccg
    38581 atcggttgcc acgataagcc gctgatgtgg caatgttttc gcagcagcga tcagttgact
    38641 ggtggaaccg accgcatccg ccatatcgac aatagcttgt ggtgattctg gatgcaccag
    38701 tatggcagca tccgggtatt cttcttgcaa gcgggttaac gcctgagtct taaattcatc
    38761 atgcacaata caggcaccct gccagcatag aatgtctcca cccgtctgtt tttgcacgta
    38821 acgccccaga tgtttgtcgg gtgcccagat gattttttca cccaaactat caagatgatc
    38881 aataagttcg acggcaatgc ttgaagttac cacccaatct gcgcgcgctt ttaccgcagc
    38941 agaagtgttg gcgtagacga cgacagtacg atcgggatgg gcatcgcaaa atgcgttaaa
    39001 ttcttcaaca gggcagccga gatccagtga acattcagcc tgaagtgtcg gcatcagaat
    39061 tgttttttcc ggactgagaa ttttggcggt ttctcccata aatctcaccc cagcgactaa
    39121 caaagtagaa gcgggatgct ttgcaccgaa gcgcgccatt tccagagaat cagaaataca
    39181 gccaccggtt tcttctgcca gttgttgaat ttcgggatcg gtatagtagt gggcaaccat
    39241 caccgcatta cgttctttta gcagacgttt tatcttctcg cggtaatacg ctttttcatc
    39301 aatgcttaac ggcgtcggct tcggggggaa aggataaatc gccgtgtctg gatcaaacat
    39361 tacgctcatc ttaccatctc gttttacagg cttaacgtta aaaccgacat tagcgtaata
    39421 ttcgctgtta tcggtgtaat ttgttttata tgctaaacaa gatagtcgaa tggtggggaa
    39481 aagtcactcg aattacgcct aattccaccg acgtagagtt gcttgcacag taagctctga
    39541 cgatgtcatc aggtttacaa agtgaaattg aggatgcctg aatagcaaaa aagcgccttt
    39601 agggcgcttt tttacattgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa
    39661 agtcaactgc tctaccaact gagctaacga ccccttgcgg gattatgctg ctttaatcat
    39721 tcaggatggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    39781 ctaccaactg agctaacgac ccatacgggt gttacctgaa agatttctac tcggggccaa
    39841 tctttaagat tggtgtgtcg tgcaggatga ctcgcttcgc ttgccctacg ggccgttgct
    39901 ggcgcaacgt tatccttcac gcttaacatc tgagtttgat gttaaaattg gtgggtcgtg
    39961 caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg
    40021 acccgagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta agggaggtgc
    40081 tctcccagct gagctaatca cccgatactg cgctggatac tacaaactac ttccaccgaa
    40141 cctaccgtaa ttagtggtgg gtcgtgcagg atgactcgct tcgctcgccc tacgggccgt
    40201 tgctgacgca acgttatcct acacgcttaa catctgtctt cgatgctaaa actggtgggt
    40261 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
    40321 aacgacccgg tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg
    40381 tgctctccca gctgagctaa tcacccaata ctacgctgga tactacaatt aactatgtta
    40441 gtggtgggtc gtgcaggatg actcgacttc gtctcgccct tcgggccgtt gctgatgcaa
    40501 cgttatcctt cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt
    40561 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact
    40621 ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt
    40681 cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga
    40741 cacgaccaga aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt
    40801 ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg
    40861 gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca
    40921 tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca
    40981 tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg
    41041 taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg
    41101 cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt
    41161 ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgcc
    41221 gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg
    41281 atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc
    41341 tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt
    41401 tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg
    41461 accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca gggggccgct
    41521 ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc
    41581 tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt
    41641 actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta
    41701 ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc
    41761 ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc
    41821 aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc
    41881 agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct
    41941 tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg
    42001 ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca
    42061 ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta
    42121 ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc
    42181 agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc
    42241 catcccctga gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc
    42301 atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat
    42361 gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc
    42421 cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt
    42481 cactttataa acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc
    42541 cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc
    42601 agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag
    42661 tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg
    42721 acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt
    42781 cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga
    42841 acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa
    42901 ctgaccgccg ttggtctgaa caacgtaggc aatacgagta cggaacgcac ctttaatgcc
    42961 ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt
    43021 gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag
    43081 ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga caactacagc
    43141 gtcaatgccc agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg
    43201 tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac
    43261 gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc
    43321 aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac
    43381 tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc
    43441 cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa
    43501 aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg
    43561 cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg
    43621 tggtttcggg atcttcgcaa gcttagctgc tgccaacgca gcctgacaaa gtgcgggatc
    43681 gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt
    43741 acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat
    43801 ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact
    43861 cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc
    43921 agagcttagc tcaccgaaaa tatcatctgc ctctgcggcc gcttttgctg cagcggcttt
    43981 ttcagctgca gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc
    44041 tttcttatca gctgctgcct tttcagcagc cgctttttct gctgcttttt tgtcggctgc
    44101 agctttctct gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc
    44161 agcttctgct ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc
    44221 agctgcttct gccgcttccg ctttcttctt cagtgccgca gcggctgcct cggctttttt
    44281 ctgcgcttcg gctgcggctt tggcggcttc tgcttctgct tttttctttg cgtctgcagc
    44341 cgctttcttc gctgcttctt ccgcagcttt agcatctgct tcggccttcg ctttagcatc
    44401 tgccgccgct ttcgctgccg cctcttcagc ttgcttctgc tttaactcgg cctgttttgc
    44461 ggcttcttca gcctgctttt tctgctcctg agccgctaac cgctctttct caagttgctt
    44521 caggcgttcc tgttcagccg cttgtttctc acgcagttct tcagcagcct gctgttcctt
    44581 catcttgcgc tgttcatcag aacgcttcgc gcttgattcc tggctttgca tgcgtttgta
    44641 ctgctcaact accgcacctg aatcaaccat gacagcgtcg atggacgaac caccgccgcc
    44701 tccggctgaa gcttctatat tctcatcgaa cgaactccag atcagcgccg caaataagat
    44761 gacatgcagc actgctgaaa taattatcgc ccgcttgagc ttgtcgtttt gttcggttgc
    44821 ctttgacact ctcggtttcc aaaaactgtt cgcctgttac ccgctctctt tcaagcaagg
    44881 gaaacgcaga tgtttagata ggctgcgtca ttaaaccaac cgatttcaca cccgcactat
    44941 gtaacaagtt cagtgcttta attatttcat cgtaaggcac atcttttgcg ccaccgatca
    45001 gaaagaccgt tttcgggttg gccttgaaac ggctggacac ttccgccacc acctgctctg
    45061 gtggtaaacg ctccaggcga tctttctcaa ccaccacggt gtactgacca ataccagaca
    45121 cttcaacaat cactggcgga ttatcgttac tgctcaccgc ctgtgattca gtagcgtctg
    45181 gcagatcgac ctccacgctc tgggtgatga tgggcgctgt cgccataaag atcagcaaca
    45241 gcaccagcag tacgtccagc aacggtacaa tgttgatttc ggacttgaga tcgcgacgac
    45301 ctcgtccacg cgctctggcc atggcttacc ccttgttgct ctcgctaacg gtaaacgcct
    45361 ggcggtgcag aatcgcggta aactcttcca taaagttgtc gtaattcagt tccagtttgt
    45421 ttacgcgctg gttgaggcgg ttataagcca ttaccgccgg aattgcggcg aacagaccaa
    45481 ttgcagtcgc aatcaacgct tctgcgatac cgggcgcaac catttgcagt gttgcttgtt
    45541 ttaccgcccc gagggcgata aaggcgtgca tgatccccca gaccgtacca aacagaccaa
    45601 tatacgggct gatggagcca accgtaccga ggaacggaat gtgcgtttcc agattttcaa
    45661 gttcacggtt catggagata cgcatagcac gcgacgcccc ttccactacg gcttccggcg
    45721 catggctatt ggcacgatgc aggcgcacaa actctttgaa cccgctgtaa aagatttgtt
    45781 ccgaaccagt cagattatcc cgtttcccct ggctctcttg atagaggcga gagagttcga
    45841 ttccagacca gaatttatct tcaaacgctt cggcttcgcg cgccgctgcg ttaagaatac
    45901 gggtccgctg gataataatg gcccaagatg cgattgaaaa accaatcaaa atcaacatga
    45961 taagtttaac cagaaggcta gccttcagga acaaatcaag gatattcatg tcagtcactg
    46021 cttaaactcc gcgacaatag acttgggaag cgcacgaggc ttcattttga gtgggtcaac
    46081 gcaaacaacc agaacctctg cttcattcag caaagtattc tcggcgttga caatacgttg
    46141 cgtgaaaacc aaagaggtgc cacgcattga tgttatttca gtctgtattt cgagcatatc
    46201 gtcgagccgc gcaggtgcgt aatattccac cgtcatttta cgtaccacaa aggcaacgcg
    46261 ttcagccatc agcgcctgct gactgaagtg atgatgacgc agcatctctg tgcgtgctct
    46321 ttcataaaaa gcgacgtaac tggcgtggta caccacacca ccggcatcgg tatcttcata
    46381 gtagacgcga accggccatc gaaacagcgt tgtattcact ttacatcccg gtaatgcaac
    46441 aaaagttaga gcttttaaac gctgctacta tacgcgcgcg attgaggttt gggaatgagg
    46501 gcaagttaag ggagcgtaaa aatttatggg cctggagcgg cccataattt tttaaagaga
    46561 attctgattt aaaagaagaa gaaaatcagc ccgacaatga gaacaatatc ggcaagcaat
    46621 gggcaaaaaa tcccttgcca aagaaccttc tgcggacgaa agcccacgcc gtgaatcaca
    46681 ccagcacaga cggcccacat cagcaataaa ccatgccaga tttccagttc actggtcttc
    46741 gcggcaaaac gagatgggtc ccaaaacata catcctgcta acagaagcgc catcacgaag
    46801 gaaagcgccc gcagggggcg cttgtccatt accgcataca aagtcgcgat aatcttactc
    46861 atcagatgtc ttcttgaccg gctttgcctg attcgacgtg ttcaagcgcc agcgcggtga
    46921 ttaccccaaa cgaacaggca agaagcgttc ccagaatcca tgcgaaatac cacattttta
    46981 gctccttact tagtacagag agtgggtgtt acgttcaata tcttctttgg tgatacgacc
    47041 gaacattttc cagtaacacc aggcggtgta gagcagaatg atcggtacca gaaccaccgc
    47101 aacccaggtc atgacgttaa gcgtcagctg gctggaagtt gcatcccaca ttgtcagact
    47161 tgcgttcatc atggtgctgg acggcatcac aaacgggaac attgcgatac cggctgtcag
    47221 gatgatgcag gccagcgtca gggaggagaa cacaaacgcc cacgcggctt tatccatacg
    47281 tgcagtcagg atggtcagca gcggcagaac cacacccagc gccggaatag cccacagaat
    47341 tggcgtgttg ttgaagttaa ccagccatgc gccagcttca cgaaccactt ctttattcag
    47401 tgggttagag gctgcgtaat ggtccattgt cgatttcacg acataaccat cgataccgta
    47461 catcacccat acgccagcca gtgcgaaaca gaccagtgtc accagcgcag ccacctgagc
    47521 cgttgcacgg gtacgcaggt gcagttcgcc cacggtacgc atttgcagat aggttgcgcc
    47581 ctgagtaatg atcatcccta cgctcaccac gcctgccagc aggccgaacg ggttaagcaa
    47641 ctggaagaag ttaccggtgt agtacagacg cagatattca tcaacgttga acggtacgcc
    47701 ctgcaacagg ttaccgaacg ctacaccaat taccagcggc ggaacgaagc taccaatgaa
    47761 gatgccccag tcccacatgt tacgccagcg ggtttcttca atcttggagc ggtagtcaaa
    47821 accgaccgga cggaagaaca aagacgccag cacgaggatc atcgccacat agaagccgga
    47881 gaacgcagcg gcatagacca tcggccaggc agcaaagagt gcgccgcccg cggtgatcag
    47941 ccaaacctgg ttaccgtccc agtgtggtgc aatggagtta atcataattc gacgctcggt
    48001 gtcgttacga ccgaggaaac gggtgagcat gcccaccccc atgtcgaaac cgtcagtgac
    48061 tgcaaaacca atcagcagaa cgccaaccag cagccaccag ataaaacgca atacttcata
    48121 atcgatcatt tgacgactcc tgtcttagcg tgccggctga gtagtcgtgg aagactgctc
    48181 aaagtgatag cgaccggttt tcaggctgct tgggccgagg cgtgcaaatt tgaacattaa
    48241 gaacaattct gccaccagga acagggtata caggccgcaa atcagcacca ttgagaagat
    48301 gagatcgcct gcggtcagtg acgagttcgc cacagctgtc ggcagcactt caccgatagc
    48361 ccacggttgg cggccatatt cagccacgaa ccagcccgct tctacagcaa tccacggcag
    48421 cggaataccg tacagcgcgg cgcgcagaag ccattttttc tcgccaatgc ggttgcggat
    48481 gacactccag aaagagagcg cgatgattgc cagcagcagg aagccacacg ccaccatgat
    48541 acggaacgca aagtacagcg gcgctacacg cgggatggag tctttggttg cctgttgaat
    48601 ctgcgcttca gtcgcatcag ccacgtttgg cgtatagcgt ttcagcagca gaccgtaacc
    48661 gaggtctttc ttcatgctat tgaactggtc acgaaccgcc tggtcggtag aaccagaacg
    48721 cagttgttcg agcagagagt acgccttcat cccgttacga atgcgttctt catgctgcac
    48781 catcagctct ttcaggccga taaccggggt atccacggaa cgcgttgcaa tgatgcccag
    48841 tgcgtaaggg atctgaatcg caaatttgtt cgtctcctct tcctgatcag gaatgccgaa
    48901 cagagtaaag gcagcaggcg caggttgcgt ttcccactcg gcttcaatag cagccagttt
    48961 ggttttctgc acgtcgccca tttcgtagcc ggattcatca cccagaacaa taacagacag
    49021 aacagcagcc ataccgaagc tggcagcgat agcaaaggag cgtttagcga aggcgaagtc
    49081 acgacctttc agcatatacc atgcgctgat accgaggatg aacatcgcgc cagtcacata
    49141 accagacgct acagtgtgaa cgaatttcac ctgagcaacc gggttaagca ccagctcgga
    49201 gaagctcacc atctccatac gcatagtttc aaagttgaaa tcggacgcga ttgggttttg
    49261 catccagccg ttcgcaacca gaatccacag tgcggacagg tttgaaccga gcgccaccag
    49321 ccaggtgaca cacatatgct gaactttacc cagacgatcc caaccgaaga agaacagacc
    49381 tacaaaggtg gattcgagga agaaggccat cagaccttcg attgccagcg gcgcaccgaa
    49441 gatatcccct acatagtggg aatagtaaga ccagttagtc ccgaactgga actccatggt
    49501 cagaccggta gccacaccca gagcgaagtt gataccaaac aacttgcccc agaacttggt
    49561 catatcttta taaatctgtt tgccggagag gacgtagacc gtttccataa tggccagcag
    49621 gaacgccata ccgagcgtca gtggcacaaa aaggaagtgg tacatcgcgg tcaaggcaaa
    49681 ctgtaagcgc gacagttcga ctatatctaa catcatgact ccttgctcat cgcatgaaga
    49741 ctccgagagt gaaccccgtt agaaagggtc acacgcatgc cccaatagaa atttgtttga
    49801 acactttcat cataccatgt gaatgcacac aaaaacggcg taaagaaagg ttgcaaacat
    49861 gttaataaaa actcaaattg atcccacgta tatattacgc cgcaaaatcc ttacaataaa
    49921 caggtttttg ttgaagcaaa tttgcatttt tcgacggtga tcaatttata gctaaattac
    49981 cgcctttcag ccaatttgat cgagaacaat ttatctcttt ttgatgccca tttccaagac
    50041 ttatacattg ataaatatca a
//
LOCUS       DAPHDX010000013        49347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPHDX010000013 DAPHDX010000000
VERSION     DAPHDX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.013"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..559
                     /locus_tag="RH058_000762"
     CDS             35..559
                     /locus_tag="RH058_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HDV3136391.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            643..1329
                     /gene="lpfB"
                     /locus_tag="RH058_000763"
     CDS             643..1329
                     /gene="lpfB"
                     /locus_tag="RH058_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HDV3136392.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1353..3884
                     /gene="lpfC"
                     /locus_tag="RH058_000764"
     CDS             1353..3884
                     /gene="lpfC"
                     /locus_tag="RH058_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HDV3136393.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3895..4950
                     /gene="lpfD"
                     /locus_tag="RH058_000765"
     CDS             3895..4950
                     /gene="lpfD"
                     /locus_tag="RH058_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HDV3136394.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4956..5480
                     /gene="lpfE"
                     /locus_tag="RH058_000766"
     CDS             4956..5480
                     /gene="lpfE"
                     /locus_tag="RH058_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HDV3136395.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5733..7424
                     /gene="eptB"
                     /locus_tag="RH058_000767"
     CDS             5733..7424
                     /gene="eptB"
                     /locus_tag="RH058_000767"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV3136396.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7516..7592
                     /locus_tag="RH058_000768"
     tRNA            7516..7592
                     /locus_tag="RH058_000768"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7550..7552,aa:Pro,seq:cgg)
     gene            7627..7760
                     /locus_tag="RH058_000769"
     ncRNA           7627..7760
                     /ncRNA_class="other"
                     /locus_tag="RH058_000769"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8505..10112
                     /gene="dppA"
                     /locus_tag="RH058_000770"
     CDS             8505..10112
                     /gene="dppA"
                     /locus_tag="RH058_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV3136397.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10263..11282
                     /gene="dppB"
                     /locus_tag="RH058_000771"
     CDS             10263..11282
                     /gene="dppB"
                     /locus_tag="RH058_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV3136398.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11292..12194
                     /gene="dppC"
                     /locus_tag="RH058_000772"
     CDS             11292..12194
                     /gene="dppC"
                     /locus_tag="RH058_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV3136399.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12205..13188
                     /gene="dppD"
                     /locus_tag="RH058_000773"
     CDS             12205..13188
                     /gene="dppD"
                     /locus_tag="RH058_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV3136400.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13185..14189
                     /gene="dppF"
                     /locus_tag="RH058_000774"
     CDS             13185..14189
                     /gene="dppF"
                     /locus_tag="RH058_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV3136401.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPALKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14219..15490)
                     /locus_tag="RH058_000775"
     CDS             complement(14219..15490)
                     /locus_tag="RH058_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3136402.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15966..16073
                     /locus_tag="RH058_000776"
     CDS             15966..16073
                     /locus_tag="RH058_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3136403.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16449..16556
                     /locus_tag="RH058_000777"
     CDS             16449..16556
                     /locus_tag="RH058_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3136404.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16932..17039
                     /locus_tag="RH058_000778"
     CDS             16932..17039
                     /locus_tag="RH058_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3136405.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17126..18805)
                     /gene="bcsG"
                     /locus_tag="RH058_000779"
     CDS             complement(17126..18805)
                     /gene="bcsG"
                     /locus_tag="RH058_000779"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV3136406.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(18802..18993)
                     /gene="bcsF"
                     /locus_tag="RH058_000780"
     CDS             complement(18802..18993)
                     /gene="bcsF"
                     /locus_tag="RH058_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV3136407.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18990..20561)
                     /gene="bcsE"
                     /locus_tag="RH058_000781"
     CDS             complement(18990..20561)
                     /gene="bcsE"
                     /locus_tag="RH058_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV3136408.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSHGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20834..21022
                     /locus_tag="RH058_000782"
     CDS             20834..21022
                     /locus_tag="RH058_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV3136409.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21034..21786
                     /gene="bcsQ"
                     /locus_tag="RH058_000783"
     CDS             21034..21786
                     /gene="bcsQ"
                     /locus_tag="RH058_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV3136410.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            21783..24401
                     /gene="bcsA"
                     /locus_tag="RH058_000784"
     CDS             21783..24401
                     /gene="bcsA"
                     /locus_tag="RH058_000784"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV3136411.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            24412..26751
                     /gene="bcsB"
                     /locus_tag="RH058_000785"
     CDS             24412..26751
                     /gene="bcsB"
                     /locus_tag="RH058_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDV3136412.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            26758..27864
                     /gene="bcsZ"
                     /locus_tag="RH058_000786"
     CDS             26758..27864
                     /gene="bcsZ"
                     /locus_tag="RH058_000786"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV3136413.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            27846..31319
                     /gene="bcsC"
                     /locus_tag="RH058_000787"
     CDS             27846..31319
                     /gene="bcsC"
                     /locus_tag="RH058_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV3136414.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSVDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            31401..33389
                     /gene="hmsP"
                     /locus_tag="RH058_000788"
     CDS             31401..33389
                     /gene="hmsP"
                     /locus_tag="RH058_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV3136415.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            33572..34858
                     /gene="dctA"
                     /locus_tag="RH058_000789"
     CDS             33572..34858
                     /gene="dctA"
                     /locus_tag="RH058_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV3136416.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            35079..36575
                     /locus_tag="RH058_000790"
     CDS             35079..36575
                     /locus_tag="RH058_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV3136417.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLTESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(36671..37600)
                     /gene="kdgK"
                     /locus_tag="RH058_000791"
     CDS             complement(36671..37600)
                     /gene="kdgK"
                     /locus_tag="RH058_000791"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV3136418.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            37832..38599
                     /gene="pdeH"
                     /locus_tag="RH058_000792"
     CDS             37832..38599
                     /gene="pdeH"
                     /locus_tag="RH058_000792"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV3136419.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            38669..40729
                     /locus_tag="RH058_000793"
     CDS             38669..40729
                     /locus_tag="RH058_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV3136420.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKVDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(40963..42285)
                     /locus_tag="RH058_000794"
     CDS             complement(40963..42285)
                     /locus_tag="RH058_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV3136421.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(42696..43709)
                     /gene="yhjD"
                     /locus_tag="RH058_000795"
     CDS             complement(42696..43709)
                     /gene="yhjD"
                     /locus_tag="RH058_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV3136422.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(43758..44657)
                     /locus_tag="RH058_000796"
     CDS             complement(43758..44657)
                     /locus_tag="RH058_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136423.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            45177..45779
                     /locus_tag="RH058_000797"
     CDS             45177..45779
                     /locus_tag="RH058_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV3136424.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRMTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(45830..47479)
                     /locus_tag="RH058_000798"
     CDS             complement(45830..47479)
                     /locus_tag="RH058_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV3136425.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSREYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            47884..49281
                     /gene="ccp"
                     /locus_tag="RH058_000799"
     CDS             47884..49281
                     /gene="ccp"
                     /locus_tag="RH058_000799"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV3136426.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        11974 a        12269 c        13390 g        11714 t
ORIGIN      
        1 tcaagcatat ttatctatat aaatggagtt ataaatgaaa aaggttctgt ttgccttatc
       61 tgcgcttgcg ctgacctcta cttccgtatt cgcagctgat gcgggcgacg gtactgttaa
      121 attcaccggt gagattgttg acgccgcttg tgttgtgtct ccagacactc aaaaacaaga
      181 agttgttctg ggtcaggtga acaagtctgt atttactgct atgggtgata aatccacagc
      241 aacgccgttc aaaatcaaac tggaaaactg cgatatctcc actttcaaaa atgtggaaat
      301 cagctttaac ggcgttggcg atgcagacaa cagcaaactg gtttctgtaa gcactgaacc
      361 aggtgccgca actggcgtag gtatcggtat ttatgataac accaataccc tggttgacct
      421 gaatactggt aaatctgcta ctgtcctgaa agaaggtcag accgttctgt atttcaccgc
      481 taactatgtc tctaccaaag atgcagtaac cacgggttac ggtaacgctg aagtcgactt
      541 caacctgact tacaactaat cgatcctgac ggaacgatca tctctcaatg gcaacagaga
      601 ctgtgttgcc attattcaca ccaatgactc agggaccgaa tcatgaaacg tattagcttt
      661 gctgtgctgg cacttttaat gaccgcacaa cagtgttttg ctggtggcgt agccttaagc
      721 acgacgcgtg ttatttatga tggcggcaag aaagaagcgt cgctgaccgt tgaaaaccat
      781 aataaaaatg aagagttttt gatccagtca tggattgatg atgtgaatgg caataaaaaa
      841 acaccgttta ttattacacc gccattattc aaacttgatc ctgataaaaa taacgtctta
      901 cgtatcgtca atattgaaga taccttacct aaagatcgtg aatccgttta ctggattaat
      961 gtaaaggcca ttcctgcgaa aagtgctgaa aatgaaaata aaaacgtctt acagatagcc
     1021 gttcgcaccc gtttgaagtt gttctatcgt ccaaaaggtt tgccgggaag caacctcgag
     1081 gccatgaaac agttacgttt ccagcgtcaa aataaccaaa ttgtggttaa taacgactcc
     1141 gcttataacc tgacctttaa tcagttttcc attaatggac aaaagattga acgggcggga
     1201 atggttctcg cgaaaggaaa gttaaacatt aaccttccag ccggtacagg caacgcgcac
     1261 gaagtgaaat acagcattat taatgatttc ggtgttgctg gcgaaatgct gacgaagccg
     1321 attaactgat ccttttacaa ggcgctttta ctatgcattg gatgcattta cctctctatc
     1381 actacagagc gcatttttcc ttctctctgc tggcattaac gattgccagc tcgttacctg
     1441 cgtatggcgg aaaatttaac cctaaatttc tcgaaaacgt tcagggtatc gatcaacaca
     1501 tcgacttgtc ggtatacgac tcacctgtcg gccagcaaat ccccggtaaa taccgggttt
     1561 cggtgtttgt aaacgaagaa aaaatggctt cccggacgct ggattttagt accgccagcg
     1621 aagcgaaacg caaagccagc ggtgagtcgc ttatgccttg ccttagccgg gtgcaactgg
     1681 aagaaatggg cgtgcgcgtt gacagctttc cggcgctgaa gatgtcacca ccagaagcct
     1741 gcgtagcgtt tgatgaaatc attccccagg ccaccagccg ctttgatttt aatactcaga
     1801 cgctgcatct ttcctttcca caggcggcaa tgatgatgac cgctcgcggc acggttgatc
     1861 cttctcgctg ggacgaagga atacctgcgc tgttgctgga ttacagcttt agtggcagca
     1921 atggacgcaa tgaaggtaca ggttcatctt cagattcgac cagtgacagt tattacctga
     1981 acctgcgcag cggtttgaac gttggtccgt ggcgtttacg taataacagt atctggaacc
     2041 gtaccgatgg caaaaaccaa tgggataacg ttggtacatc gttaaaccgg gcaattattc
     2101 cacttaaatc acagataact ctgggtgata ccgccacgcc tggcgagatt tttgatagcg
     2161 tacaaatgcg tggggcgtta ctggcatccg acgatgaaat gttgccggac agccagcgtg
     2221 gctttgctcc ggtagtgcgc ggcatcgcca aaagcaacgc tgaagtgagt atcgaacaga
     2281 acggctatgt catttatcgc acttttgttc agccgggtgc atttgagatt aacgatctct
     2341 acgcaacctc cggcagcggc gacctgacgg tgatcattaa agaagccgac ggaagtgaac
     2401 aacgttttat ccagccgttt tcggcggtgg cgattttcca gcgtgaaggg tatctgaaat
     2461 acagtctggc tgccggtgaa tatcgtgctg gtaattacga cagcgataaa ccacatttcg
     2521 ggcagtttac cgccatgtac ggcttgccgt ggggtatgac cgcctacggt ggtgcattgt
     2581 tgtcggcaga ttacagcgct ttagctctgg gcctggggaa aaacttcggt accattggcg
     2641 cagtatcggt ggatgtcact caggcgaaaa gtcagttacg caacaatgaa aaagaggaag
     2701 gccagtctta tcgcttcctc tattcgaaaa gttttgaggg tggcactgac ctgcgtctgt
     2761 tgggctacaa atactcaacc agcggctact acaccttcca ggaagcaact gatgttcgca
     2821 gtgacgccga cagtgattac cgccgttatc acaagcgcag ccagatacag ggcaatatta
     2881 cccagcaact gggagactac ggctcggttt atttcaatat gacgcagcag gattactgga
     2941 acgttgacgg taaggaaaac tccttaagcg ccggttatca cggtcacatt ggtcgggtga
     3001 attacagcgt ggcctatacc tggacgagaa gtccggaatg ggaagaagac gatcgcttgt
     3061 ggtcattcag tgtttccatc cctctggggg gcgcgtggag cagttatcgc atgactaccg
     3121 accagaatgg caagacgtcg caacaggcct ctgttagcgg cacgttactg gaagatcgta
     3181 acctgagcta taacgttcag cagggttaca ccagtaatgg cgtaggctac agtggcagcg
     3241 tcaacatggg gtatatgggt ggctctggta acattgacgt aggctataac tattctaaag
     3301 ataaccaaca ggttaactat ggcgtgcgcg gcggtgtcat cgttcacagt gaaggtataa
     3361 cgctttcgca gcctttaggc gaaagtctgg cgattgtttc tgcaccgggc gcgcgtggtg
     3421 gacatgtggt taacagcagc ggcgtagaag ttgactggat gggcaacgcc gtggtgcctt
     3481 atctcacacc atatcgtgaa accattgtgg aattgcgtag cgacacgttg gggcaaaacg
     3541 ttgaactaca ggaagcgttc cagaaagtcg tgcctactcg tggtgcagtt gtgcgttccc
     3601 gctttgatac ccgcgtcggt taccgtgtgc tgatgagctt aaaacaggcg aatggcaacg
     3661 cagtaccgtt tggtgcaacc gcagcgttaa ttgatgagag caaacctgcc agcagcattg
     3721 tgggtgaaga aggacagctt tatatcagcg ggatgccaga agagggcgaa ttgcaggtga
     3781 gctgggggaa cgagcaggcg caacgttgcc gtgtgccatt ccgcttgcca gagaacaagg
     3841 acaatgcggc tatcgtgatg gttaatgcgg tatgcgagaa ataaggatat ttagatgaaa
     3901 attaaaaatt tgttggcagg tatggtgctg ttagggacga gcacttttgc tggaaatata
     3961 tgggctgcgg attggggacc atgtcagaca tcaaatgggg tggcccatga gtatagtttt
     4021 gatttcgtac agactatcca ggtaccttca gaaaataagg ctgggaaaat attaactcag
     4081 ccctttgctt taggtacaaa atattccgct tattgtgaat gcccggatcc cattccagat
     4141 aatggagttg tgacttattt taaaggtgta acgcttctgc ctgagcctgg aactgtagat
     4201 gggtactata aattcaataa ttacattgat attctcacca aaatatatgt ctacacacaa
     4261 ttagatatcc cggtgccttt taccgatcga agcaatggca cggcacagaa agaatgtacg
     4321 ccctatactg ctaacaattg ggggacaggt ggtaaaggaa gtatcagtat ttatatttca
     4381 catccttttg ttgggcaaat gattattcct aaaacacgag ttgcgtccct ctttgggaca
     4441 aaaaagaaag gtgtttacaa tgatagtcag cccatggcca atgtcagtat ttccgggagt
     4501 attacagtta ctcaaggctg tgaacttgcc gctggcaccg tactggatat tccattcgga
     4561 gaatatcagg cccatgattt taaagggcgt gccggacaac caccacaaaa tgttcagaag
     4621 gtacaaaaag aattaagctt tgactgcaat aatatttcag atggcgtcaa aatttatctc
     4681 agtattgatg caacaccaaa tactgcctat cccagcgcaa ttgatttagg caacgccgat
     4741 gttggtgcag tcattgagga cggtaaaggt aatattctta agccgaatga cagtaatagc
     4801 ttattagaaa tgaacccagg ctcgctgtat gaagatgtca aacgcaaagc gacaaccaca
     4861 attaccgctt atcccgttag cactaccgga aaattacccg ctgccgggga ttatagcggt
     4921 attgctacca tacatgtcga actggagtaa cagtcatgaa gcgaacaata cttgcgttat
     4981 gcctgctgcc tctttccttt tcgtcattcg cggcaaccac ggatttaggg ccgaaaggaa
     5041 cattgaaatt cacccttaaa atcagccagg gtgcttgcga actggtgaaa gacagcgttg
     5101 aggttgacat gggcaccgcg gttctcaaaa agccggtaag aatcggaacg gaaattaatc
     5161 ccactccttt ttccatcggc ctgaaaaact gtagcgaagt ggtcagagcc tacgtgacta
     5221 tggacggtac acccaatgca gacgactcaa atctttttgc gctggatgcg ggtggcgcta
     5281 caggcgttgg gctgaaaatc aaaaccgctg ctggagtaca acagttcccg gaaaatacaa
     5341 cgccgacacc ggttgagttt gcaattaacg gtgatggcgc acatcaactg aactatatcg
     5401 ccagctacgt gcctattcgc gccgatgcaa ctattggtcg cgcagatgca acggtcgact
     5461 ttactgtaca gtacgaataa tttcacatct taagggccag ttaactggcc ctttttactg
     5521 ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg tgcttcatgc
     5581 acactctttc cccacacttt ttcgctttgc tgtggtctac ttattcgcgc gtgtagattt
     5641 tacttatctg actacctccg cacttttccc ctgccgggcc tgaaaagccg ctaagcaggg
     5701 tgttatcacc tgtttgtcca gggtttgttt gcatgagata catcaaatcg attacacagc
     5761 agaagctgag ctttttgctt gcaatctata ttggcctttt tatgaatggc gcggtttttt
     5821 atcgccgctt cggcagctat gcgcacgatt ttaccgtctg gaaaggcgtt tctgctgttg
     5881 ttgaactggc cgccaccgta ctggtgacct tctttttact acgtcttcta tcgctgtttg
     5941 gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca ggtgccagct
     6001 attacatgac cttccttaat gtggtcattg gttatggcat catcgcttcc gtcatgacca
     6061 ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt tattctctgg ttaatcgccg
     6121 ttagtgcatt gcctcttatc cttatctgga ataaccgctg tcgctacacc ttgctccgac
     6181 aactgcgaac cccagggcag cgtattcgca gcctggcggt cgtcgtactg gcgggtatta
     6241 tggtttgggc accgattcgt ttgctggata tccagcagaa gaaagtggag agggcgaccg
     6301 gcgttgattt gccgagttat ggcggtgtcg tagcgaactc ttatctgcca tcaaactggc
     6361 tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga atcttccgat aataattcat
     6421 tgcttaatcc ggcgaagaaa ttcacttatc aggcaccgca aaacgttgat gacacttatg
     6481 tcgtgtttat catcggtgaa accacgcgtt gggaccatat gggcattttc ggctatgagc
     6541 gtaataccac gccgaaactg gcccaggaga aaaatctggc ggcgttccgt ggttactcct
     6601 gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt acgtcagggg ggcgcggaag
     6661 ataatccgca gcgcacatta aaagaacaga acattttcgc ggttctgaag cagttaggat
     6721 tcagttctga cctctacgct atgcagagcg aaatgtggtt ctacagcaac acgatggcgg
     6781 acaacattgc ttatcgtgag cagattggtg cggagccacg taatcgtggc aagccggtag
     6841 atgatatgtt gctggtagac gaaatgcagc aatcgctagg gcgcaacccg gatggtaagc
     6901 atctgatcat tctgcatacc aaaggttcgc attttaacta cacccagcgt tatccgcgta
     6961 gcttcgcgca gtggaagccg gaatgtattg gtgttgatag cggctgtacc aaagcgcaga
     7021 tgatcaactc ctatgacaac tcggtgacct atgtggatca ctttatctcc agcgtgattg
     7081 atcaggttcg cgataagaaa gcgattgtgt tctacgcagc tgaccacggt gagtcaatta
     7141 atgaacgcga gcacctgcac ggcacgccgc gtgaactggc accgccggag cagttccgcg
     7201 taccgatgat ggtctggatg tcagataaat atctggaaaa tccggccaat gctcaggcat
     7261 ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg ccgtcatgta gagctgtacg
     7321 acaccatcat gggttgtctt ggctatactt caccggatgg tggaattaac gaaaacaaca
     7381 actggtgtca catcccgcag acaaaagtgg cagcggctaa ctaagcggcc tgctgacttt
     7441 ctcgccgatc aaaaggcatt ttgctattaa gggattgacg agggcgtatc tgcgcagtaa
     7501 gatgcgcccc gcattcggtg attggcgcag cctggtagcg cacttcgttc gggacgaagg
     7561 ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa aagcctgctc agcgagcagg
     7621 cttttttgca tttgaagccc atgaggatga gaacctccgg gggcggggag gttcgactcg
     7681 agcgaagcga gagaacgttg cgccagcaac ggcccgcagg gcgagccacg aagtggcgag
     7741 taatcctcta tcaccgacca aattcgaaaa gcctgctcaa cgagcaggct tttttgcatt
     7801 tgtagctcat gaggatgaga acctccgggg gcggggaggt tcgactcgac gtaggcctga
     7861 taagacgcgt cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga tgcggcgtga
     7921 acgccttatt cgacctacac gccacacatt tccctgccaa atttgcgccc catatctcac
     7981 tatccattaa cggatttgtg acagaaacca ttgtcttttt ttatatttgc ttaaatctat
     8041 tttttcgtaa cttatggctg gcgctggcat tttccgctgt gcgttttagc gttcatggta
     8101 ttaatcgctc agatatccac cttttcctca gatttttttc actaaatgca taaaacttaa
     8161 tcaccaaatg ttgctaaata ttaagctgat tgttctggtc atgttggagt agcacaaatt
     8221 tctctgctgc aaatagctgt ttgaagtttt tttaatcttt gtttgtggat tctcaccgtt
     8281 ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga ttgtaggtca
     8341 cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt acggcatccc
     8401 cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca
     8461 ctgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt atttccttga
     8521 aaaagtcagg gatgctgaag cttggtctca gcctggtggc tatgaccgtc gcagcaagtg
     8581 ttcaggctaa aactctggtt tattgctcag aaggatctcc ggaagggttt aacccgcagc
     8641 tgtttacctc cggtaccacc tatgacgcct cttccgtacc gctttataac cgcctggttg
     8701 aatttaaaat cggcaccacc gaagtgattc cgggcctcgc tgaaaagtgg gaagtcagcg
     8761 aagacggtaa aacctatacc ttccatctgc gtaaaggtgt gaagtggcac gacaataaag
     8821 aattcaaacc gacgcgtgaa ctgaacgccg atgacgtggt gttctcgttc gatcgtcaga
     8881 aaaacgcgca aaacccgtac cataaagttt ctggcggcag ctacgaatac ttcgaaggca
     8941 tgggcttgcc ggagctgatc agcgaagtga aaaaggtgga cgacaacaca gttcagtttg
     9001 tgctgactcg cccggaagcg ccgttcctcg ctgacctggc aatggacttc gcctctattc
     9061 tgtcaaaaga atatgctgat gcgatgatga aagccggtac accggaaaaa ctggacctca
     9121 acccaatcgg caccggtccg ttccagttac agcagtatca aaaagattcc cgtatccgct
     9181 acaaagcgtt tgatggctac tggggcacca aaccgcagat cgatacgctg gtcttctcta
     9241 ttacccctga cgcttccgtg cgttacgcga aattgcagaa gaatgaatgc caggtgatgc
     9301 cgtacccgaa cccggcagat atcgctcgca tgaagcagga taaatccatc aatctgatgg
     9361 aaatgccggg gctgaacgtc ggctatcttt catataacgt gcagaaaaaa ccactcgatg
     9421 acgtaaaagt tcgccaggct ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg
     9481 tttatcaggg cgcgggtgta tcagcgaaaa acctgatccc gccaaccatg tggggctata
     9541 acgacgacgt tcaggactac acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag
     9601 cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa cgtccgtata
     9661 acccgaacgc tcgccgcatg gcggagatga ttcaggcaga ctgggcgaaa gtcggcgtgc
     9721 aggccaaaat tgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa gatggcgagc
     9781 accagacggt aatgatgggc tggactggcg ataacgggga tccggataac ttcttcgcca
     9841 ccctgttcag ctgcgccgcc tctgaacaag gctccaacta ctcaaaatgg tgctacaaac
     9901 cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac
     9961 tgtacaaaca agcacaggtg gtgatgcacg atcaggctcc ggcactgatc atcgctcact
    10021 ccaccgtgtt tgaaccggta cgtaaagaag ttaaaggcta tgtggttgat ccattaggca
    10081 aacatcactt cgaaaacgtc tctatcgaat aattaaaagc catacccggt ggcgctgtgc
    10141 ctctgcgtgc agcgccatcc ggcagcaata cttcattctc tgtccgacaa gggcgggaat
    10201 tgatttgtga gcaatacaga cacgcagttc caggctgcgg gtcattatag agaatccggg
    10261 ttatgttgca gtttattctc cgacgtttgg gactcgtcat ccccacgttt atcggtatta
    10321 cccttctcac atttgccttt gtccacatga tcccgggcga tccggtgatg atcatggcgg
    10381 gcgaacgtgg gatctcccct gagcgtcacg cgcagctgct ggctgaactc ggcttagata
    10441 aaccgatgtg gcagcagtat ctccattaca tttggggcgt aatgcacggc gatctaggca
    10501 tttcaatgaa aagccgaatt ccagtatggg aagagttcgt gccgcgcttt caggccacgc
    10561 tggaacttgg cgtctgcgcg atgatttttg ctacggcagt cggtattccg gttggtgtgc
    10621 tggctgcggt taaacgcggt tccattttcg atcacactgc agttggcctg gcgctgaccg
    10681 gctactcgat gccgatcttc tggtggggca tgatgctgat catgctggtt tcggtgcact
    10741 ggaacctgac gcccgtctcc ggtcgcgtga gcgatatggt gttcctcgat gactccaatc
    10801 cgttaaccgg ttttatgcta atcgacaccg ccatctgggg tgaagacggc aactttatcg
    10861 atgccgttgc ccatatgatc ttaccggcca ttgtgctggg tactattccg ctggcggtca
    10921 ttgtacgtat gacacgctcc tcgatgctgg aagtgctggg tgaagattac atccgcaccg
    10981 cgcgcgccaa agggttgacc cgcatgcggg tgattatcgt ccatgcgctg cgtaacgcga
    11041 tgctgccggt agtgaccgtt atcggcctgc aggtgggaac attgctggcg ggggcgattc
    11101 tgaccgaaac catcttctcg tggcccggtc tggggcgctg gttgattgac gcgctgcaac
    11161 gccgcgacta tccggtagtg cagggcggcg tattgctggt ggcgacgatg attatcctcg
    11221 tcaacctgct ggtcgatctg ctgtacggcg tggtgaaccc gcgtattcgt cataagaagt
    11281 aaggggacat catgtcacag gttactgaaa ataaagtgat tagcgcaccg gtgccgatga
    11341 ccccgttaca ggagttctgg cactatttta aacgcaacaa aggcgcggtc gtcgggctgg
    11401 tttacgtcgt catcgtgctg ttcatcgcga tctttgccaa ctggattgca ccctataacc
    11461 cggcggaaca gttccgcgat gcactgctcg ccccgccagc ctggcaggaa ggcggcagca
    11521 tggcgcactt gctgggcacc gatgacgtag gccgtgatgt gctgtcgcgc ctgatgtacg
    11581 gtgcgcgcct gtcgctgctg gttggctgtc tggtagttgt gttatcgctg attatgggcg
    11641 ttattctcgg cctgatcgcc ggttactttg gcggcctggt cgataacatc attatgcgcg
    11701 tggtcgatat catgctggcg ctgccaagtc tgctgctggc gctggtgctg gtggcaattt
    11761 tcggcccgtc gattggtaac gccgcgctgg cactgacctt cgttgccttg ccgcactatg
    11821 tgcgcttaac ccgcgccgcc gtgctggtgg aagttaaccg cgattacgtc accgcgtctc
    11881 gcgtggcggg tgccggggcg atgcgtcaga tgtttattaa catcttcccg aactgccttg
    11941 cgccgctgat tgttcaggcg tcgctcggtt tctctaacgc cattctcgat atggctgctc
    12001 ttggtttcct cggcatgggg gcacagccgc caacgccgga gtggggcacc atgctctccg
    12061 acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac cttcccgggt ctggcgatcc
    12121 tgctgacggt gctggcattt aacctgatgg gtgacggtct gcgtgacgcg ctcgatccca
    12181 aactgaagca gtaagaggtt cgagatggcg ttattaaatg tagataaatt atcggtgcat
    12241 ttcggcgacg aaagcgcacc gttccgcgcc gtagaccgca tcagctacag cgtaaaacag
    12301 ggcgaagtgg ttgggattgt gggtgagtcc ggctccggta agtcggtcag ttccctggcg
    12361 attatggggc tgattgatta tccgggccgc gtaatggcgg aaaaactggt gtttaacggt
    12421 caggatttgc agcgtatctc ggaaaaagag cgccgcaacc tggtgggtgc cgaagtggcg
    12481 atgatcttcc aggacccgat gaccagcctt aacccgtgct acaccgtggg tttccagatt
    12541 atggaagcga ttaaggtgca tcagggcggc aacaaaagta cccgccgtca gcgggcgatt
    12601 gacctgctga atcaggtcgg tattcccgat ccggcttcgc gtctggatgt ttacccgcat
    12661 cagctttccg gcggcatgag ccagcgcgtg atgatcgcca tggcgattgc ctgtcgacca
    12721 aaactgctga ttgccgatga gccgaccacc gcgctggacg tgaccattca ggcgcaaatc
    12781 atcgaactgt tgctggagct acagcagaaa gagaacatgg cgctggtgtt aattacccat
    12841 gacctggcgc tggtggcgga agcggcacat aaaatcatcg tgatgtatgc aggccaggtg
    12901 gtggaaacgg gcgatgcgca tgccatcttc catgcgccgc gtcacccgta tactcaggca
    12961 ttgctgcgtg cgctgccgga atttgctcag gacaaagaac gtctggcatc gttgcctggc
    13021 gtcgttcccg gcaagtacga tcgcccgaac ggctgcctgc ttaacccgcg ctgcccctat
    13081 gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata tgctcgctga cgggcgtcag
    13141 tccaaatgcc attacccact tgatgatgcc gggaggccca cactatgagt acgcaagagg
    13201 ccacctcgca acaaccgctg ttgcaggcga ttgacctgaa aaaacattac ccggtgaaga
    13261 aagggatgtt tgcgccggaa cgtctggtga aggcgctgga cggcgtttcg tttaaccttg
    13321 aacgtggcaa aacgctggcg gtagtgggcg aatctggctg cggtaaatcg accctcggtc
    13381 ggttgctgac gatgatcgaa acgcccaccg gcggtgagct gtattaccag gggcaggatc
    13441 tgcttaagca cgatccgcag gcgcagaagc tgcgtcggca gaaaatccag atcgtcttcc
    13501 agaatcccta cggttcgctg aatccgcgta aaaaagttgg gcaaattctc gaagagccgc
    13561 tgcttattaa caccagctta agcaaagaac agcgtcggga aaaagccctg tcgatgatgg
    13621 cgaaagtcgg cctgaaaacc gagcattacg accgctatcc gcacatgttt tccggcggac
    13681 aacgtcagcg tatcgctatt gcccgtggtc tgatgctcga cccggacgtg gtgattgccg
    13741 atgaaccggt ttctgcgctg gacgtctcgg tgcgcgcgca ggtgctcaac ctgatgatgg
    13801 atttgcagca ggaactgggg ctttcttatg tctttatctc gcacgacctg tcggttgtgg
    13861 agcacattgc cgatgaagtg atggtgatgt acttgggccg ctgcgtggag aaggggacga
    13921 aggaccaaat cttcaataac ccgcgtcatc cttacactca ggcgctgctc tccgcgacgc
    13981 cgcgcctgaa cccggacgat cgccgcgagc gcatcaagct caccggtgaa ctgccaagcc
    14041 cgctgaatcc tccgccgggt tgcgccttca acgcccgctg tcgtcgacgc ttcggcccct
    14101 gcacccagtt gcagccagca ctgaaagatt acggcggtca actggtggcc tgctttgccg
    14161 ttgatcagga tgaaaatccg cagcggtaat gttcaaaacc ggagccttgt gctccggttt
    14221 actcaatcag cttcaacagt ggcgacaagc atagcaaaat gccgtacagc agaatcagcc
    14281 aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt atagatcagg aaaaacggaa
    14341 tgaggcacga cacaatgcca tataacgggc ttcccaactg aaagaacacc agcaccgata
    14401 cacgaaacga aacccaaatc gtcaacgtaa tgacgataaa agcgcagatc gccagagtca
    14461 gcacgcgtga gttaattttc ttggtatcaa taatacggct taacagattg agaataatgc
    14521 ctttaatggc ctcgtggaaa ccgagataaa tgccaaagaa tgcggtcagt acggcaaaga
    14581 tattcagtac cgtagaggtg atatgaatga tatgcccagg gatcacctgc gcggccagtg
    14641 ccagtgccga gatattttgt tcaaaggcag aaacggcttc ttcgtggcta attgagaagg
    14701 taaacgaaaa ggcaaaaaac agtatcaccg cgatgagcgt gacataacta attcggtggg
    14761 tacgcagcgc gagccgggtt gccagtactt tatccgcttc ccgtttacga taggcaatat
    14821 tcattgggtt aagtacctga ataaataccg cagaaaagaa gcaaaaggga atggtaagca
    14881 ggacatcgcg gaaaaagacg gaggcttgcg ggaaggcggt tatattggcg aaattccagt
    14941 gcgggatcat cgcaaaaccg aacacgacaa taatccctac tttgaccacc accattggcc
    15001 cagaaatctt aaacagtaat cgttcaccac cagacgcaat cgccaccagt acggcgaaaa
    15061 tagcgacttt ataaagtaga gattgtgaaa gatcggcatc ggttaaaccg aaggttttca
    15121 ggtacgaggc gctgtcgaaa accacggaga gagagtagat aaaaatcccg tggataatca
    15181 tcaaaaagta gataaccccg aggaaaattc cccagttctt ccccaggtaa tgactgataa
    15241 tatcggtgta gtcattacag gaatcgcttt cagaaagggt ttttaaataa atgtcctgca
    15301 ctacccaagt ggcaggataa gcaatgatcg ctgcggtaat aaatacccaa attcccttca
    15361 agccaatttg tactggcatc agcacggttc cggcaccaat cgccatgcct atgcataata
    15421 aaacccagcc gaagtcgtag cgtgtaaacg gcaatttctg atcgtgtttc ggtagtgtgt
    15481 tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg caaaaatgaa taaacccaga
    15541 ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat gtaaaaaatg aggcaacaga
    15601 ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat tgcgcatttc actaccagtc
    15661 tggagtcagc ccggatatga actgcaacta ttgcaaatta agaaattaag ctggaaggca
    15721 tcacgcaatg aggaagattg ccgggtgctg acagtaaaaa gcaatcgtaa aatcagcggc
    15781 aagccggaag ggttccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    15841 ttgctggaga gagaaaaccc ccgcacgttg caggtatata cctgacaaca taacgggggc
    15901 taatcttgaa cctagaccac tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg
    15961 gctatatgac gctcgcagag ctgggcatgg ccttctggca tgatttagcg gctccggtca
    16021 ttgctggcat tcttgccagt atgatcgtga actggctgaa caagcggaag tagcgtgtca
    16081 tgtgggcgtc aggctgccgt aatggcaatt tgcgcccgga ccaggccgca ggagtgaaac
    16141 tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag cggataatat tctgcggaat
    16201 gcgcagcaca gagtgaagat gagacggatt ccggaacaga aaaacagtcg tagaatcagc
    16261 gtcaagccgg aaggattccg gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat
    16321 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gcacctgaca acaccacggg
    16381 ggccattctt gcatccaaac aatacaagaa tagccgcgaa acgttgtcat tacaatacag
    16441 gcggctatat gacgctcgca gaactgggca tggccttctg gcatgattta gcggctccgg
    16501 tcattgctgg tattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtaacgtg
    16561 ccatgcgggc gtcaggctgc cgtaatggca atttgcgccc ggatcaggcc gcaggggtga
    16621 aactctgcgg cctttttcgt aagaaaaatg caacggttgc aagcggataa tattctgcgg
    16681 aatgcgcagc acagagtgag taagtgatgg attccggaac agaaaaacag tcgtagaatc
    16741 agcgtcaagc cggaagggtt ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    16801 cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacatcac
    16861 gggggccatt cttgaacctg aacaactcaa gaatagccgc gaaacgttgt cattacaaca
    16921 caggcggcta tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc
    16981 cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaataag cggaagtaac
    17041 gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcagagg
    17101 ttaaactctg cggccttttt cgtgcttact gcgggtaagg cacccagtcg ccgccgttca
    17161 ggcgaacgta cggtttatcc tggtattgaa taactactgc atttgagttc tcggagaccg
    17221 gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa
    17281 tcttgccatc aagaacgcga accaccagat cggagatagc caggaagctg ctcggttggt
    17341 caatgacaat cggtgccccc tgatgtggtg ccttcatgcc gaagaatttc accccaacgg
    17401 ggacgtctgt gatagacggg ctagggatat cacgtaggcc agatacctgc attctgtcgc
    17461 ccttcagcgc gccgccgtgt tccggcacca cgaccaccat cactttacga cccgatttct
    17521 ccagttcagt aaagaaggcg tccagttcat caaagaattt ctgcgcccgc gctttgtaat
    17581 ccgctgtttt gctgaccccc ggataatggt tgccgtcatg cagtggaagc gtgttgtaga
    17641 acgtggcact acggctgttt ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg
    17701 cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa aataaccggc agatttgttt
    17761 gatccatcaa ttcagtctgc atgccgccat tttcgcgaac ttctttcaaa aaaccgccga
    17821 actggccgtt atgccccatc atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa
    17881 acagatagca gtcgttattt gccggttgat acagattagt gtgcgaagtc tgcccgcagc
    17941 tggcgcgcag taaacggatc gccgccgggc cactgtagga ggtggcggag ttaaagttct
    18001 tgaactcaat atcgaaatgc gaccacagtg gatgcgacat caacccggcg gcttctatat
    18061 ccgaccagga aagcgaacag atgttaatca ccagtagttc aaatggctga gcatcagcgg
    18121 gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc gttatagaaa ttattcagcc
    18181 aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc gggcatatca cccactaccg
    18241 gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc acccgtcgtt gttacagtgg
    18301 tcgtcggttg tccggctggc cacaaggaga aacttggtcc cgccagggta agtacgttca
    18361 gccatagcag tatggcaacc acaaaaacgg taatgcgaat ccattgtgac aggaataacc
    18421 aggccactaa taaaacaaaa atggccccaa tcatctgcca gttaataaag cgtgtgacaa
    18481 ggtcgattaa ataatcggta ctgaaccccg ccacctgcga accctggctc attatgcttt
    18541 ccgggccagg caaccaggtg tcatgccaga acaaagcaaa gccgatcggc agggcaatcc
    18601 agtggcgcaa gcgatgcagg ctgtagcgcg gaatgggcat cagcagaaac gcggcaaaca
    18661 ccaaattgag gagcggatgg aagttaagat atcccgccca caacaggccg aacttaacca
    18721 gaaaatagaa gttccagccg gaaaggccgc gccagtattg ccagagggaa gaaggcatgg
    18781 cggtattttg tgtaaattga gtcatttttt ggttgccctg gctttttccg tgcggcgtaa
    18841 cgtcccggcc ggtttaacat aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat
    18901 gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa aatatcagtg cgcaaacgac
    18961 aataatttca atgatatcgc tgatggtcat catgatgagc gctccacagc atcatctaac
    19021 agtcgcatgg gttctggtat tcgtcgccag tggcgaccat cgtgctcggc attgatgacc
    19081 ggtttagaac tttgcgttaa aggcagcggc atgccccatt gttctggggc aagcaagcgc
    19141 atctgcacca gctcggcact gatttgatca tcttcaaacc agaccatacg gtttgagaaa
    19201 atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg tatccagatc gttaatccga
    19261 cagaatgaga gaaacagcac cagccgatta ccgccaatgg tcatgatatc gccggtacgg
    19321 ttagggcgac acagcgtcag ggcttgttca acgcggatac ccggtaccgg acgtagggca
    19381 accagaacgc ctttaccgtg ggcaggcaag agagggttat tcatcatgtt gttgacggca
    19441 ttacagaaca catcccactt ctggaaacca cgcagtttga gcggctgggt cattgacagc
    19501 aaggtagtga tatcttccgg tacatagcga ctaaacttct gcccttgcac gctttcgatc
    19561 atcgtcagac aacgggagag tggcgcattc cacggaataa ccatatttgc accgcaggcc
    19621 aataacaaac gttcatcggt ggcgcgcagg ctagcggtat tttcccgcac gaggattttc
    19681 atcgcactac cgcgctggcg acgcagggta tgaatgctgc gggccagtgg ctcgatttgc
    19741 gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag cggtacgggc ttcattgaac
    19801 aggacttcgt tattgttgaa cagttgccag tgttccgata gcggcggcgc accttccagt
    19861 acagcaacat tactcagaat gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg
    19921 ctttgaacta atgtccagat accattttgt tgctgaacgc taagctgctg acgggcgctg
    19981 accccttttt cgttgcacca gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc
    20041 aaactggcaa gaccaaaaag tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg
    20101 ttattatttc cgggattaat taccaaaagc gaacaatggt ttaacctgct ccatttattc
    20161 attttatcca accatgagcg aagccgctcg gcaggaatgt tttgccatgc gttatttgcg
    20221 caaacaagaa taaaaaggta attatgggga tcaatagaac actgcaaatc acgggtcaaa
    20281 tagtatagac ctttttcatg atttagcatt gaaaataatt ttattttttc cggcccgtga
    20341 gaatcatcta attgaaagat ttttgccgga tcgctgtcca tgctaatgac cgcgacgtgt
    20401 gcggtttcag cctgggatgc aattgtttga ttcgccagac tgatagcatc ttcatggcga
    20461 tcgacgttaa accaccagac gccgcctgct ggcatatgtc gcagctcatc ccataatgat
    20521 gagataccga tagagaatac agggtccaca atgtccctca tgtcaccatt ttgtctgatt
    20581 atcagtttac tagcgaaagc taagaaataa acctaccatt gaaattaaaa cttatcagat
    20641 ttagcatgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
    20701 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
    20761 aataaaccgg cgaaatgctc acggttagaa ttgtctcatg aacggtacag ttatttcata
    20821 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
    20881 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
    20941 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
    21001 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
    21061 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
    21121 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
    21181 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
    21241 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
    21301 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
    21361 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
    21421 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
    21481 gatgccaact gccatatccg cctgcatcag caagcgctgc cggatggcgc acatattttg
    21541 attaataact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
    21601 agccagcgcc gattgctgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
    21661 gcggctaagc aaccagtcgg tgaataccgc agtgatgcgc tggcggcaga agagatactg
    21721 acgctggcga actggtgcct gttgaactat tccgggctga aaacgccagt cgggagtgca
    21781 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
    21841 gttatcgcga ttatcgtcgc cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
    21901 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctggcag cgtattcgcg
    21961 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
    22021 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
    22081 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaatgga
    22141 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
    22201 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
    22261 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
    22321 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
    22381 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
    22441 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
    22501 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtcgtatgg ccgctgaatc
    22561 gtcagccggt gccattgccg aaagatatgt cgctgtggcc gtcggtggat atctttgtcc
    22621 cgacttacaa cgaagatctc aacgtggtga aaaatactat ttacgcctcg ctgggtatcg
    22681 actggccgaa agacaagctg aacatctgga tcctcgatga cggcggtagg gaagagtttc
    22741 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg taccactcat gaacatgcga
    22801 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
    22861 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
    22921 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccagac ccgtttgaac
    22981 gcaacctggg gcgttttcgt aaaacaccga acgaaggcac gctgttctat ggtctggtgc
    23041 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctgtgcg gtgattcgcc
    23101 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac tgtgactgaa gatgcgcata
    23161 cttctctgcg tttgcaccgt cgtggatata cctccgcgta tatgcgtatt ccgcaggcgg
    23221 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
    23281 ggatggtgca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
    23341 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
    23401 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
    23461 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
    23521 tccagggcaa atatcgccac tctttctgga gtgaaatcta cgaaacggtg ctggcgtggt
    23581 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
    23641 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
    23701 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
    23761 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
    23821 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
    23881 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
    23941 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
    24001 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
    24061 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
    24121 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
    24181 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
    24241 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
    24301 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccgcccg gagcggagcg
    24361 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
    24421 aaactattct ggatttgtgc agtggctatg gggatgagtg cgttcccctc tttcatgacg
    24481 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
    24541 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
    24601 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
    24661 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
    24721 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
    24781 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
    24841 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
    24901 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
    24961 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
    25021 tatcagaccc tgaatgtgaa gaatgacctg tcacacttcc cggtgccatt ctttgatccg
    25081 cgtgataacc gcaccaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
    25141 caacaagcct ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
    25201 aacttcccgg tgctctataa ccaactgccg gatcgtaatg ccattgtctt tgcaaccaac
    25261 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
    25321 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
    25381 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
    25441 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
    25501 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
    25561 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
    25621 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
    25681 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
    25741 agcagcaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
    25801 gatggcaaaa cagatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
    25861 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
    25921 cagccggtgc agaatcatgt ggtgattggt gacgactcca ccatcgactt ctcgaagtat
    25981 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccattcagc
    26041 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aagcgcctaa cgaagcacag
    26101 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
    26161 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
    26221 atcggtggta tcccggacaa actgaaagac gataagcaga tcgacctatt ggtgcaggcg
    26281 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
    26341 gagagcgatc gcgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
    26401 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
    26461 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
    26521 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
    26581 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
    26641 ccgattctgc tggcggtgct ggcggctatc agtgtgatat tgctggcatg ggtactgtgg
    26701 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
    26761 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
    26821 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
    26881 gtcatcgacc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
    26941 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
    27001 aacaatctcg ctcagggttc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
    27061 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
    27121 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
    27181 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
    27241 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
    27301 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
    27361 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
    27421 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
    27481 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
    27541 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
    27601 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccggt cggtttttct
    27661 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
    27721 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
    27781 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
    27841 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
    27901 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
    27961 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
    28021 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
    28081 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
    28141 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
    28201 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactgt
    28261 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
    28321 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
    28381 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
    28441 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
    28501 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
    28561 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
    28621 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
    28681 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
    28741 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
    28801 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
    28861 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
    28921 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
    28981 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
    29041 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
    29101 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
    29161 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
    29221 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
    29281 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
    29341 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
    29401 gcaatctggc gcagcagaag ccgaacgacc cggagcaggt ttacgcttac gggctgtacc
    29461 tctctggtca tgaccaggac agagcggcgc tggcgcatat caacagcctg ccgcgcgcgc
    29521 agtggaacag caatattcag gagctggtta atcgactgca aagcgatcag gtgctggaaa
    29581 ccgctaatcg cctgcgagaa agcggcaaag aagctgaagc ggaagcgatg ctgcgtcagc
    29641 aaccaccttc cacgcgtatt gacctcacgc tggctgactg ggcgcagcag cgacgtgatt
    29701 acaccgccgc ccgcgctgca tatcagaatg tcctgacgcg ggagccaact aacgccgatg
    29761 ccattctggg gctgacggaa gtggatattg ctgccggtga cacagcggcg gcacgtagcc
    29821 agctggcgaa actgcccgct accgataacg cctcgctgaa cacacagcgg cgcgtggcgc
    29881 tggcgcaggc gcagcttgga gataccgcag cggcgcagca gacgtttaat aagttgatcc
    29941 cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat ggtgctgcgc gacggtgcga
    30001 agtttgaagc gcaggcgggc gatccaaagc aggcgctgga aacctacaaa gacgccatgg
    30061 tcgcatccgg tgtgactacg acgcgtccgc aggataacga cacattcacc cgactgaccc
    30121 gtaacgacga gaaagatgac tggctgaaac gcggcgtgcg cagcgatgcg gcggacctct
    30181 atcgccagca ggatcttaac gtcaccctcg agcacgatta ctggggttcg agcggcaccg
    30241 gtggttactc cgatctgaaa gcgcacacta ccatgttgca ggtggatgcg ccgtattctg
    30301 acgggcggat gttctttcgc agtgatttcg tcaatatgaa cgtcggcagt ttctccacta
    30361 atgccgatgg caaatgggat gacaactggg gcacctgtac attacaggac tgtagcggca
    30421 accgcagcca gtcggattcc ggtgccagcg tggcggtcgg ctggcgaaat gacgtctgga
    30481 gctgggatat cggtaccacg ccgatgggct tcaacgtggt ggatgtggtc ggcggcatca
    30541 gttacagcga tgatatcggg ccgctgggtt acaccgttaa cgcccaccgt cggcccatct
    30601 ccagttcttt gctggccttt ggtgggcaaa aagactcccc gagcaatacc gggaaaaaat
    30661 ggggtggcgt acgtgccgac ggtgtggggc taagtctgag ctacgataaa ggtgaagcaa
    30721 acggcgtctg ggcatcgctt agtgtcgacc agttaaccgg taaaaatgtc gaagataact
    30781 ggcgcgtgcg ctggatgacg ggctattact ataaggtcat taaccagaac aatcgccgcg
    30841 tcacaatcgg cctgaacaac atgatctggc attacgacaa agatctgagt ggctactcac
    30901 tcggtcaggg cggttactac agtccgcagg aatacctgtc gtttgccata ccggtgatgt
    30961 ggcgggagcg cacggaaaac tggtcgtggg agctgggggc gtctggctcg tggtcgcatt
    31021 cacgcaccaa aaccatgccg cgttatccgc tgatgaatct gatcccgacc gactggcagg
    31081 aagaagctgc gcggcaatcc aacgatggcg gcagcagtca gggcttcggc tacacggcgc
    31141 gggcattact tgaacgacgt gttacttcca actggtttgt tggcacggca attgatatcc
    31201 agcaggcgaa agattacgca cccagccatt tcctgctcta cgtacgttat tccgccgccg
    31261 gatggcaggg tgacatggat ttaccgccgc agccgctgat accttacgcc gactggtaag
    31321 ttttcagata gcgcctctct taatgccgct gcgatcgggt atactcgggc ggcaatctgg
    31381 gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt aacaatcaag cagatggcaa
    31441 tggtggcagc cgttgtcctg gtgttcgttt ttattttttg caccgttttg ctgttccatc
    31501 tggtccagca gaatcgctat aacacggcta cgcaactgga aagcattgct cgctctgtcc
    31561 gcgaaccctt atcttcagct attttgaaag gcgatattcc cgaagcggaa gctattcttg
    31621 ccagcattaa accggcaggc gtggtcagcc gtgccgatgt agtgctgcct aaccagttcc
    31681 aggcgctgcg taaaagtttt attccagagc gcccggtgcc ggtaatggtt actcgcctgt
    31741 ttgagctacc ggttcaaatc tcgctgggcg tttactcgct cgaacgtccg gcaaacccgc
    31801 agccaattgc ctatctggta ctacaggcgg attccttccg tatgtataag ttcgtgatga
    31861 gcaccctctc aacgttagtg accatttact tacttttgtc gcttatcctg accgtcgcca
    31921 tcagctggtg cattaaccgc ctgattttgc atccgttacg caatattgct cgcgaactta
    31981 acgccatccc agcccaggag cttgttggtc accaactggc attaccgcgt ctgcatcagg
    32041 acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa ccagcaattg ctgcagcgcc
    32101 attatgaaga acagaacgaa aatgcgatgc gcttcccggt gtcggatttg ccgaacaaag
    32161 ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa acaaaccacc gcgctgatga
    32221 tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct gaaagaggcg caacgagaaa
    32281 ttctgctgct gacgctggtg gaaaaactca aatcggtact gtcgccacgt atgatcctcg
    32341 cgcagattag cggttatgac tttgctgtca ttgccaacgg tgtacaggaa ccgtggcacg
    32401 caatcacctt aggtcagcaa gtgctcacta tcatgagcga gcgcctgccg attgaacgta
    32461 ttcaactccg tccgcactgt agcattggcg tggcgatgtt ctacggcgat ctcaccgccg
    32521 aacagcttta cagtcgcgct atttctgcgg catttaccgc tcgccataaa ggcaagaatc
    32581 agattcagtt ctttgatccg cagcagatgg aagccgccca gaagcggttg acggaagaga
    32641 gcgatatcct taatgcactg gaaaatcatc agtttgctat ttggttacag ccacaggtcg
    32701 agatgaccag cggaaaactg gtcagtgcgg aagtgttact gcgtatccag caaccggatg
    32761 gcagttggga cctgccggat ggcttaatcg atcgcattga gtgctgtggg ctgatggtta
    32821 ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct tgcagcctgg caagagcgcg
    32881 gcattatgct gcccttgtcg gtaaacctct ctgcgctgca actgatgcac ccaaatatgg
    32941 tggcggatat gctggaactg ttaacccgct atcgcattca gccgggaaca ctgattctgg
    33001 aagtgacaga aagccgacgt attgacgacc ctcatgctgc ggtggcaatc ctccgtccgc
    33061 tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg catgggctac gcagggctgc
    33121 gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact gaaaatcgac aaaatgtttg
    33181 ttgaaggctt gccggaagat agcagcatga ttgctgcaat tatcatgctg gcgcagagcc
    33241 tgaacttaca aatgattgcc gaaggcgtgg agactgaagc acaacgcgac tggctggcaa
    33301 aagcgggcgt tggtattgcc cagggcttcc tttttgctcg cccactccct attgaaatct
    33361 tcgaagagag ttacctggaa gaaaagtagc taccccaaac tgattacaaa actttaaaaa
    33421 gtgctggttt gtgcgagcca gctcaaactt tttaaccttt ttgtttcaat tatgatccag
    33481 gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc gcaggtaccc cataacctta
    33541 caagacctgt ggttttacta aaggacaccc tatgaaaacc tctctgttta aaagccttta
    33601 ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct atcctgaaat
    33661 aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga tgatcatcgc
    33721 tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca tgaaggcggt
    33781 cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca tcgcgctgat
    33841 tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg tcgatccggc
    33901 aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc agggcattgt
    33961 cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca gcggtaacat
    34021 tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc tgggcagcaa
    34081 aggccaactg atttttaacg tcatcgaaag tttctcgcag gtcatcttcg gcatcatcaa
    34141 tatgatcatg cgtctggcgc ctattggtgc gttcggggca atggcgttta ccatcggtaa
    34201 atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct acattacctg
    34261 tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt tcagtatctt
    34321 caaatttatc cgctacatcc gtgaagaact gctgattgta ctggggactt catcttccga
    34381 gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgca aatcggtggt
    34441 ggggctggtc atcccgacag gctactcgtt taaccttgat ggcacatcga tatacctgac
    34501 aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg tccaccaaat
    34561 cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa cgggtagtgg
    34621 ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag cgggtctggc
    34681 gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctgacta acctggtcgg
    34741 taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc acaaaaaact
    34801 ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat cctcttaatc
    34861 tcaccactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata attgacccct
    34921 gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg tgacattttc
    34981 atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc gacaacgggc
    35041 agttttttta ttaccaggat agttgatcag gggttcacat gcagggcaca aaaattcgac
    35101 ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc
    35161 agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga
    35221 ccacccccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc
    35281 tcaccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc
    35341 aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg gcagcagggg atcgacccta
    35401 aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc
    35461 ccaataaccg taacgatttg ctgaaagaag cgctctctta tctggcaaat gccactggca
    35521 aattgaccat cacaccagaa accatcaacc acgcgctgca aagtcaggac atggtggcaa
    35581 cctggcctgc cgatactaaa gagggctggt ggcgctatcg tctgaaaggg tcaaccttgt
    35641 taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaaa attaaagatt
    35701 tctatcagaa atggtacacc ccggatgcaa tgacgctact ggtggtggga aacgtggatg
    35761 cgcgctcggt tgtcgaccaa ataaacaaaa cgtttggcga actgaaaggc aaacgtgaaa
    35821 cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgactg
    35881 acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg
    35941 aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgcgaggcg ctgttctggc
    36001 atgttcagca agcgttaagc gccagtaaca gcaaagacat cggtcttgga tttgactgcc
    36061 gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga
    36121 acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg
    36181 aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa ctgtttgccg
    36241 cctatgcgcg ggctgatacc gatattctga tgggtcagcg gatgcgttcg ttgcaaaatc
    36301 aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg gcaggatttc cttaacagcc
    36361 tgacggtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa
    36421 tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg
    36481 atcaaatcat ggccccatcg actgctgctg ccgccacctc tgtcgccacg gatgacgtac
    36541 atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca
    36601 accatcaggt atttatggat gagctggata gaggggtaac actttatcca gccttttgca
    36661 tatgctgcgt ttacgctggc atcgcctcac gcgggataat cgcgccgcga tactgaataa
    36721 cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg cccgtcaaac
    36781 gtaccgccag ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg atcacttttt
    36841 ctttcggcag tttcaccgcc ggaacatcca ctaacccttc gccagcaatg gacaccaggc
    36901 aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa
    36961 tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc atcgtccagc gtcaggaagg
    37021 cgatatccgt gcattccagc atttgttggt acacctgctg tgtctcttct ttgctggccc
    37081 acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gccgttggcg cggcattcgc
    37141 gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc
    37201 tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac tgctcgctct
    37261 ccagccagaa tttggcagcg gcttcgttac gccagtagta gaacgtacgt tcaccggtgc
    37321 tgtcggtttc aatgtagtaa aggcccggca gacggttttc catccgttgg gtcagggaag
    37381 tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg gctaaaactg tccgttccca
    37441 gcgccgttac gtaatggacg gttaatgccg caggatcgac ctgacgggcg atatagacgg
    37501 aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct
    37561 caatcatgca ttcgccaatc acggcaatct ttttggacat agtcgtgaac ctgatctgta
    37621 aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt
    37681 ccattatttt tttgagccag gacgtattcc tgaaagattc cgttgtggag tgaggaaatt
    37741 taccccggcc aggcggtaaa gttctgccct tacgcgccga taatctttgt cgagtccggg
    37801 cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag
    37861 caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg
    37921 tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt
    37981 aacggtggtc gcgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac
    38041 tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca
    38101 aaaagccgac ttctttatag agcacggcct gctggcatcg gtcaatattg atggccctac
    38161 gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc ttccctggct
    38221 gcgtttcgaa ctggtggagc atatccgtct gccgaaagat tcaacctttg cctcgatgtg
    38281 tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact
    38341 aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca
    38401 gtcgccggaa ggacgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg
    38461 cggggtgatt gttgagggcg tagaaacgcc ggaagagtgg cgtgatgttc agaactcgcc
    38521 cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatagaaa cgctgaatac
    38581 ggcggttctg gcgctataag ctgcctcatt ttccgcctgg ctggactatc tttaggactg
    38641 gcacaggaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat
    38701 ttcagtggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg
    38761 gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc
    38821 tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag
    38881 ctgggtgccg tggccccatg tacacgcgga agacatcatt cttggcaatc caccggatat
    38941 tcccgaagtc acgatggtgc atttgccacg cgtagaggca acgctggccc cgctggcgct
    39001 gctgaccaaa acggtctggc tgccgtggat caagctcgaa aagcccgacg cgcgcctgat
    39061 tcgcctctcc gaaaagaaca ataactggac gtttaatctc gccaacgatg ataacaaaga
    39121 cgcgaatgca aagccgtcgg catggtcgtt tcggctggat aatattcttt tcgatcaagg
    39181 gcggatcgct attgatgaca aagtaagcaa agcggatctg gagatttttg ttgatccgtt
    39241 aggcaagccg ctgccgttca gcgaagttac tggatcgaaa ggtaaagcgg ataaagaaaa
    39301 ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac
    39361 gggtacggga aaaataggcg gtatgctggc gctgcgtggc gaagggacgc cgtttccggt
    39421 acaggctgat ttccgctctg gtaacacccg tgttgctttt gatggcgtcg tgaatgaccc
    39481 aatgaagatg ggcggtgtcg atttacggct taaattttct ggcgattcac tgggtgatct
    39541 ctatgaactg acgggcgttc tgctgcccga taccccgccg tttgaaacgg atggtcggct
    39601 ggtagcgaaa gtcgacactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg
    39661 aattggcgat agcgatatcc acggttctct ggtctacacc accggaaagc cacgaccaaa
    39721 actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttaattgg
    39781 cgttgactcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa
    39841 aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga
    39901 cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gtagcctgcc
    39961 gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct
    40021 gaaatttggc atggcgggcg gcagcattgc ggcgaatatc catctggaag gcgataaaaa
    40081 gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc
    40141 cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactacgcgg
    40201 tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat
    40261 gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta
    40321 cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg cggcgaatct
    40381 gaatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt
    40441 gattaatgtt accggcacgg caagttttgc ttcggaacag ctggatttga ctattgatcc
    40501 ggagagtaaa gggattcgga ttatcacact gcgttcgccg ctgtatgtgc gtgggacgtt
    40561 taaaaatccg caggctgggg tgaaagccgg gccgttgatt gcccgtggtg ctgttgctgc
    40621 ggcactggca acgctggtaa caccggcggc ggcgttactg gcactgatct caccttccga
    40681 aggggaggct aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat gaggttggat
    40741 tgtgtttgct ggtgagttcg ggagcagcgt gttcccctca ccctaaccct ctccccaaag
    40801 gggcgagggg actgttttgt gcgtgttgaa acaacgagta ccgcttttcc gttcactttc
    40861 tgggggaggg ctgttatgtt cgcgatataa catctcgctc cgcttttctc cctctccctt
    40921 tcagggagag ggctggggtg agggtggctg cccacacgat acttacaacg actgatgtcg
    40981 cgtctcatgg gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatataa
    41041 ccccaccgca cctaacccgt agttagtctg caaccaggct gcgatatacg gcgcaacgga
    41101 agccccgaga atcgacgcta cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt
    41161 tgggaacagc tctggtaaca gcgcgcccat cggcccgaag gtcagcccca tcagacttaa
    41221 ccccagcagc aggaaggcaa aaaccagaat cgggttgcca gaaccgagca gcgggttaaa
    41281 ggcgaacagc gcgaacagga tgatcagcgt ggtgataatt accatgcttt tacggcgacc
    41341 aaaggcatca gccagtaatc cggcgactgg caccatcacg ccaaaaccaa taactgccat
    41401 catcagcatc cacaacactt cgttacgtgg caggccaagc ccaactggcg cggcggcggt
    41461 actgaaggtc atagagtaga ccgtcatgat gtaaaacagc gtatatgttg ccagcatgat
    41521 gaacgtaccc agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt
    41581 cacctgtttt ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg
    41641 aacatacagg ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca
    41701 gctcataaac tgctcatcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa
    41761 gaagaagcca atcggtgcgc ccagctgtgg gaaggagcca tacagtgcac gtttgcgcgg
    41821 tggggcgttt tcagtcgcca gcagcgccgc gccgccccat tcaccgccta agcccagacc
    41881 ctgaccaaat cgagccagcg ccagcagcag tggggcgaaa ataccaatcg tggcatagcc
    41941 cggcagcaga ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt
    42001 cgctttacgc ccaacgcgat cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc
    42061 gacgaaggcg atggcgaagg tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg
    42121 cgggaagaag atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa
    42181 gaactcaatg gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg
    42241 cgtgtattct tgctcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt
    42301 tttggctaac gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat
    42361 cgcggaaatg gtcaaaaacc aggcaaaaag gggataatgt ttaggccagt gatttttctt
    42421 ctatgaaatg cttcacataa ttttataaat agtggataaa actggtttca ggttaaataa
    42481 aagttaccaa ctggatttat atgctggggt tatgagttgg aatgaaattg tttgctccca
    42541 aatagatggc tgaagaggct ctggggaaga gtttcaagag gttatgtgcg cattatagcc
    42601 ggatgcgatg ctggcgcatc ttatccggcc tacggggagc caggccgaat aaaatcgtat
    42661 gccggataag gcgtttacgc cgcatccggc ctattttaag gctgcgtttt ccctggcatt
    42721 cgcgggtcgt ctttatattc ggcggtggca atccacgccg cgcaaaacag cgtcaaacgg
    42781 gcgaagaagt agaaaaatgc catcagcccc agcacggaac caaatgctgc gccagagggg
    42841 gatttcatca gcgatggcaa ggtgtaggtc atcacgattt taatcacttc aaaaccaata
    42901 gcagccagaa atgttccgcg aatcagggct tttttgcgcg gacggtggcg cggcaaccgc
    42961 cagaagatcc agaagaaaag cagatagttg gcgaagatgg aaatcgccag gccaatcaac
    43021 cgccacgtcg gtttcagcca ctcaatgctg ttcaggtgca gggcgctaat aatcatttgc
    43081 tgcgccgaac cggcaaccga ggtgatcgaa agcgtcacaa tcagcgcaat caacaaacca
    43141 atcagcgaaa taaaatcacg cagatatttt acccagaact tctcctgatc ttgcggcgag
    43201 cgttcccaga catcgcgcga ctgggcacga atcgcttcac gcaggttacc catccagttg
    43261 atgccggaat aaagcgccac cgccaggccg acaagcccta cagtcgtacg ctgctgaacg
    43321 gcggtgttga tggtgttttt caacgtggcg gctagcgtcg gatcgctgat gttttgcaga
    43381 attttgtcga agatatcctg tagcaacatc ggatgggagg ccagcacaaa gccccctgcg
    43441 gcaaacgaca ccatcaaaat cgggatcatc gataaaaatg agaaataggt gatagccgca
    43501 ccaaactggt tacccagccg atcgttaaag cgttctgtcg cgcgaatcag gtgcgcaata
    43561 accggttggc gttggacttt ttcggcagtg gtggtgacgg tttccagcgc ctggctaact
    43621 ttgccgcctt tctcgctatt atccaactgc ttaatcggct cgtgctccag atcctgggtg
    43681 ggacgtttga tctcgttttc ctgcgtcatc cgctcttttg tccttctgtc gtggtcattc
    43741 ttaaaaagta tagtcggtca gtccaggtgc tctttcatta cgccagccag ccactgcata
    43801 aacaggttta cacgccggga aagctcccga cgctgcgggt aaaccagcga aagggagagc
    43861 ggctcggcac ggtagcctgg caacacttca ataagccgcc cggcacgcag ggcttcgcgc
    43921 acggcaatgc gcgggatctg aataatcccc agaccggcaa gaccggcggc gagatagttt
    43981 tcgctactgt ttaccgtcaa catgccgcca gacttaaacc actggacgcc attaacgctg
    44041 gcaacctcaa aacctaacgg atgtacaccc aggtgcggtg tataacgcac tattgcgtgt
    44101 gaagtcagat catcggggct ttgcggataa ccaaagcgcg tcagatagtg cggactggca
    44161 cagttgacca tggtcagttt gccgagggga cgggcgataa cgccatcttc cggtaacgcg
    44221 ccagtgcgta tcacgcaatc aaaaccatca tgaagaatat ctaccggacg gtcatggcta
    44281 ctcagttcca gctcaattcc cggatgcaga tagagaaatt ccgacaggcg cggcagtaac
    44341 aggcttttcg cgattcctgg cgggatgtcg atgcgtaatt taccgctgat actggtggca
    44401 tcctgttgaa acagaccgtc cagttcgctg agattactca acacatcttt tgctcgttga
    44461 taataggtca tgccttctgg cgtgagtttg acccgtcgcg tggtgcgctg gagaagctgg
    44521 gtgccaagtt gatgttccag tgcctgtatc tggcgcgaaa cacttccttt tggcaaagca
    44581 aagaaatccg ccgcgcggga aaaactttcc agctccgcga ctttgatgaa caactgcatt
    44641 gcgtgaattt tatccatttc tttgaccgat tgttgttcac agcgaaacag taacgcgcat
    44701 atcgcattgt ttattgtttt ttctaacatc taatgccggg tttcacaatg ttgtcatgac
    44761 gaatgtggcg atttctcagc aggaaaaggg cggaatagct ttctgagaaa ggtgaatccc
    44821 cctaaacaga gcgtattttt cagcgattcc ctttaaagtg aatagaggac gtgttggcta
    44881 tttgtgctta tttttgtctc attgccgttt gttatttttg cttgtggtcg cggagtttca
    44941 tttttaattg catgcgtact aaccaccaat ataaggaaat cctcaaaatt gttttaaata
    45001 gtgccctcga catgtaattt caggcgttta gctgtaacta tttatatcgg taactatttt
    45061 ctacgcgtca tctctgtaga atggccaccg ccaaatggcg aattcttatt ttttctcgcg
    45121 ggtaataccc ttccactact taagtctttt taacgtattc aggtggagat tcagttatgc
    45181 aaatagtcat gtttgacagg cagtcaatat ttattcatgg aatgaaaatc agtttacagc
    45241 agcgtattcc aggagtgagt attcaggggg ccagtcaggc agacgagtta tggcaaaagc
    45301 tggaaagtta ccctgaagcc ttagttatgc tcgatggcga tcaggatggt gagttttgct
    45361 actggttgct gcaaaaaacc gtggtgcagt ttcctgaggt taaggtgtta atcacggcga
    45421 cggattgcaa taaacggtgg ttacaggaag ttatccattt taatgtgctg gccattgtgc
    45481 ctcgtgattc aaccgtcgag acatttgcgt tggcggtaaa tagtgcggcg atggggatga
    45541 tgtttctgcc tggagactgg cggatgacgc cggaaaagga cattaaagat ctcaaatcgt
    45601 taagcgcccg ccagcgggag attttaacca tgttagcggc gggcgaatca aataaggaga
    45661 tcggcagagc gctgaatatc agtaccggaa cggtaaaagc gcatctggaa tccctgtatc
    45721 gccgtctgga agtgaaaaat cgcacccagg cggcaatgat gttaaatatc tcctcctgaa
    45781 gaggataatt ttacagccag caggaagtta gcggctggct gtaaaaatat tatggttcgc
    45841 cgtacaaacc aattaaacgg cgtaccacac cgttagtcca gccaaaccca tcctgcaacg
    45901 gatactcgcc accgccgcct tcgcggggaa caccatcggc aatatggtat ttctcgatca
    45961 ttttgtgctg ttccagatag aactgattca ccgttttcag ccagctgcgc gcgatttcat
    46021 cacccagaag gtcatcgcca tacattttaa atccctgaat tgccatccat tgtaacggtg
    46081 cccagccatt gggtttatcc cactgttcac cggtttcgta ctcgcttgcc agaatcccgc
    46141 caggtgtcag taaccggctg cgtacggcgt ttgccagacg atcggcctgt tcatggttcg
    46201 ccatgccgac atagagcggc acaatggcgg cagcggaaaa cagcgccagt tgttcgcgtc
    46261 gccagtcgta atcgcggtag atgccgtttt catcatccca gaggtaacgg tttaccgcat
    46321 cgcgacgggc actggccttc tggcggaaca gcgcttctgt ctctttctcg cctttcagcg
    46381 ccgagatgtt ggcgatggcg ctctccagtt taaacaggaa ggcattcaga tcgatgggga
    46441 tgaactgggt ggtacgaatg ctcgccagac gaccagtatc acgcagccaa cgggaagagt
    46501 aatcccaacc tgaggccgct cccgcgcgta aatcgcggta cacctcgttg ggcgggcgac
    46561 cagaatgttt cgcggtttca acgtcctcaa gccaggattc gtcacgcggc gtgtcgcgat
    46621 cgtcccaata acggttgagc agcgatccgt ccggcatccg cacaacatgg cgataggcct
    46681 gattagggat caacgattct gcaccgtcca tccagaaggc atattccatt ttaaggtggt
    46741 cgagatagcg gcgcgcaccg cgtacaccat cttcttcaaa caactccacc atcagcgcaa
    46801 aaaccggtgg ttgcgatcgg ctcaaataat aggtgcggtt gccgttgggg atgtgaccat
    46861 agttttcgat catccaggcg aagttatcgg ccatgcattt cagcaaatct tcccgaccac
    46921 tttccgccag ccccagcatg gtgaaatagg aatcccaata gtaggtttcg ctaaaacggc
    46981 cgcccgggac aatatatgac tgcggtagcg ccagcagaga agaccacgga atgtgatcct
    47041 gtggttcgcg ggttagcacc ggccacagct ggtcgatatg ctctttcagg gaattttgcg
    47101 ggtccgatac atactctctg gagtagacct ccggcagcca gaagtgattt tcaacaaact
    47161 tgcgcaagtc aaaatcacga tgacggcgca ctttacggta gcggattaaa atatccagcg
    47221 ggtccatttt gggtgcgcag tcgggaaagg ttttactgtc ggggaaaatt ttcgccgact
    47281 gaacatgctc gaacagttcg agatagcgat cggcaggcgt cagcgcatca gaggcgggca
    47341 gcccttcaat catctcgggt tccggttctg cctcgatcat ctcatccagt tttaattcat
    47401 acgggtccag ctcatagcag agatcgactt cgatcatcag ttcgtctgga ttagggtttt
    47461 gaattttctg attgagcata aggatccaac ctccgaaatt cgtcgctaat tatcgggctt
    47521 ttctttaagg gtagtcattc gctccgcctg gtgggcgaca aagcgtgcgg tggatcacgt
    47581 ttattaacct gtcaaaaaat ataacgcact aatatccatg gaatttataa aaatacggat
    47641 ttcgtgttgt ttttcatttt cagaacattc aatcctgctc tcgccgcaat catgatatgt
    47701 cattcctgat ctgggtaata ggcacaggct atcttattga tagtttatat tcatgtaatt
    47761 gattgattcc tatcaaaaag cgtatctccc tgttttttat gatggctttg tctcgcagag
    47821 acgtttgcag cattctggaa aattttctgg gtcacaataa ctcaccaccg tgagggaaga
    47881 ataatgaaaa tggtctcacg tattaccgcg atcggcctgg ctggcgtcgc gatttgctat
    47941 ttagggttat ctggttatgt gtggtaccac gataataaac gcagtaaaca ggccgatgtt
    48001 caggcatctg ctgtcagtga aaataataag gttttaggct ttctccgcga aaaaggatgc
    48061 gactattgcc acacaccttc ggcagaatta cccgcctatt attatattcc tggcgcgaaa
    48121 cagttgatgg attacgacat taagcttgga tataaatctt ttaacctcga ggccgtgcgt
    48181 gcggcactgc tggctgataa acccgtttcg caaagcgatc tgaataagat tgaatgggtg
    48241 atgcagtatg aaactatgcc accaacgcgt tataccgcgc tacactgggc gggtaaggtg
    48301 agtgatgaag agcgggcgga aatactggcc tggattgcaa aacagcgcgc ggaatattac
    48361 gccagcaatg atactgctcc ggagcatcgc aatgaaccgg tgcagcccat cccgcaaaaa
    48421 ctgcctaccg atgcgcaaaa agtggcgttg ggctttgcgc tgtatcacga tccccgttta
    48481 tcggctgata gcaccatttc atgcgctcat tgccatgcgt tgaatgcggg gggcgtcgat
    48541 ggcagaaaaa catcgattgg tgttggtggc gcagttgggc cgattaacgc gccgacggta
    48601 tttaactcag tatttaacgt tgagcagttc tgggatggtc gtgcggcaac attgcaggat
    48661 caggcaggtg gaccgccgtt gaacccgatt gaaatggcgt cgaaatcctg ggacgaaatt
    48721 attgctaagc tggaaaaaga tccgcagctt aaagcgcagt tcctcgaagt ttatccgcaa
    48781 ggtttcagtg gcgaaaatat tactgatgcc attgctgaat ttgagaaaac attaattacg
    48841 ccggattccc catttgataa atggttgcgt ggagatgaaa atgcgctgac ggcgcaacag
    48901 aaaaaaggct atcaattatt taaagataat aaatgtgcaa cttgtcatgg tggtattatt
    48961 ctcggcggac gttcctttga accgttgggg ctgaaaaaag actttaactt tggggaaatt
    49021 acggcggcgg atattggtcg tatgaatgtg actaaagaag agcgtgataa attgcgtcag
    49081 aaagtacccg gtttacgtaa cgttgcttta acggcaccgt acttccatcg cggtgacgtg
    49141 ccgacgctgg acggggcggt gaaactgatg ctgcgctatc aggtaggcaa agagctgccg
    49201 caggaggatg tggatgatat cgtagctttc ctgcacagtc tgaacggggt gtacacgccg
    49261 tatatgcagg ataaacaata attaatttga tcgcccgaac aggaatgttt gggcgatttt
    49321 tattacgata ataaagtctg tttttaa
//
LOCUS       DAPHDX010000014        49177 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPHDX010000014 DAPHDX010000000
VERSION     DAPHDX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.014"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(183..1130)
                     /gene="dusC"
                     /locus_tag="RH058_000800"
     CDS             complement(183..1130)
                     /gene="dusC"
                     /locus_tag="RH058_000800"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV3136427.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1369..1767
                     /locus_tag="RH058_000801"
     CDS             1369..1767
                     /locus_tag="RH058_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV3136428.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1764..2459
                     /locus_tag="RH058_000802"
     CDS             1764..2459
                     /locus_tag="RH058_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV3136429.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2589..3473
                     /gene="cdd"
                     /locus_tag="RH058_000803"
     CDS             2589..3473
                     /gene="cdd"
                     /locus_tag="RH058_000803"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV3136430.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3623..4342
                     /gene="sanA"
                     /locus_tag="RH058_000804"
     CDS             3623..4342
                     /gene="sanA"
                     /locus_tag="RH058_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV3136431.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPTDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4345..4584
                     /locus_tag="RH058_000805"
     CDS             4345..4584
                     /locus_tag="RH058_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV3136432.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     IWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4903..6141
                     /gene="preT"
                     /locus_tag="RH058_000806"
     CDS             4903..6141
                     /gene="preT"
                     /locus_tag="RH058_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV3136433.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            6135..7370
                     /gene="preA"
                     /locus_tag="RH058_000807"
     CDS             6135..7370
                     /gene="preA"
                     /locus_tag="RH058_000807"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV3136434.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELCDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7441..8451)
                     /gene="mglC"
                     /locus_tag="RH058_000808"
     CDS             complement(7441..8451)
                     /gene="mglC"
                     /locus_tag="RH058_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV3136435.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8467..9987)
                     /gene="mglA"
                     /locus_tag="RH058_000809"
     CDS             complement(8467..9987)
                     /gene="mglA"
                     /locus_tag="RH058_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV3136436.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(10048..11046)
                     /gene="mglB"
                     /locus_tag="RH058_000810"
     CDS             complement(10048..11046)
                     /gene="mglB"
                     /locus_tag="RH058_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV3136437.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11326..12366)
                     /gene="galS"
                     /locus_tag="RH058_000811"
     CDS             complement(11326..12366)
                     /gene="galS"
                     /locus_tag="RH058_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV3136438.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(12508..13665)
                     /locus_tag="RH058_000812"
     CDS             complement(12508..13665)
                     /locus_tag="RH058_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV3136439.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13682..14350)
                     /gene="folE"
                     /locus_tag="RH058_000813"
     CDS             complement(13682..14350)
                     /gene="folE"
                     /locus_tag="RH058_000813"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV3136440.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14608..15444
                     /gene="yeiG"
                     /locus_tag="RH058_000814"
     CDS             14608..15444
                     /gene="yeiG"
                     /locus_tag="RH058_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV3136441.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15476..17549)
                     /gene="cirA"
                     /locus_tag="RH058_000815"
                     /pseudo
     CDS             complement(15476..17549)
                     /gene="cirA"
                     /locus_tag="RH058_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(17842..19311)
                     /gene="lysP"
                     /locus_tag="RH058_000816"
     CDS             complement(17842..19311)
                     /gene="lysP"
                     /locus_tag="RH058_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV3136442.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19516..20397)
                     /locus_tag="RH058_000817"
     CDS             complement(19516..20397)
                     /locus_tag="RH058_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136443.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20496..21545
                     /locus_tag="RH058_000818"
     CDS             20496..21545
                     /locus_tag="RH058_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV3136444.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21619..22476
                     /gene="nfo"
                     /locus_tag="RH058_000819"
     CDS             21619..22476
                     /gene="nfo"
                     /locus_tag="RH058_000819"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV3136445.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22480..23568
                     /locus_tag="RH058_000820"
     CDS             22480..23568
                     /locus_tag="RH058_000820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3136446.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23624..24874)
                     /locus_tag="RH058_000821"
     CDS             complement(23624..24874)
                     /locus_tag="RH058_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV3136447.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24974..25915)
                     /gene="rihB"
                     /locus_tag="RH058_000822"
     CDS             complement(24974..25915)
                     /gene="rihB"
                     /locus_tag="RH058_000822"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV3136448.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            26045..26743
                     /gene="yeiL"
                     /locus_tag="RH058_000823"
     CDS             26045..26743
                     /gene="yeiL"
                     /locus_tag="RH058_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV3136449.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26811..28061)
                     /locus_tag="RH058_000824"
     CDS             complement(26811..28061)
                     /locus_tag="RH058_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV3136450.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLVGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28155..29093)
                     /gene="psuG"
                     /locus_tag="RH058_000825"
     CDS             complement(28155..29093)
                     /gene="psuG"
                     /locus_tag="RH058_000825"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV3136451.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNITV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(29081..30022)
                     /locus_tag="RH058_000826"
     CDS             complement(29081..30022)
                     /locus_tag="RH058_000826"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV3136452.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(30446..32137)
                     /gene="fruA"
                     /locus_tag="RH058_000827"
     CDS             complement(30446..32137)
                     /gene="fruA"
                     /locus_tag="RH058_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV3136453.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(32154..33092)
                     /gene="fruK"
                     /locus_tag="RH058_000828"
     CDS             complement(32154..33092)
                     /gene="fruK"
                     /locus_tag="RH058_000828"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV3136454.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(33092..34222)
                     /gene="fruB"
                     /locus_tag="RH058_000829"
     CDS             complement(33092..34222)
                     /gene="fruB"
                     /locus_tag="RH058_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV3136455.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34590..35771
                     /gene="setB"
                     /locus_tag="RH058_000830"
     CDS             34590..35771
                     /gene="setB"
                     /locus_tag="RH058_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV3136456.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35768..36022)
                     /locus_tag="RH058_000831"
     CDS             complement(35768..36022)
                     /locus_tag="RH058_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3136457.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36177..36749
                     /gene="yeiP"
                     /locus_tag="RH058_000832"
     CDS             36177..36749
                     /gene="yeiP"
                     /locus_tag="RH058_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV3136458.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36972..38438
                     /locus_tag="RH058_000833"
     CDS             36972..38438
                     /locus_tag="RH058_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV3136459.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38556..39542
                     /gene="yeiR"
                     /locus_tag="RH058_000834"
     CDS             38556..39542
                     /gene="yeiR"
                     /locus_tag="RH058_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV3136460.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39581..40294
                     /gene="lpxT"
                     /locus_tag="RH058_000835"
     CDS             39581..40294
                     /gene="lpxT"
                     /locus_tag="RH058_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV3136461.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40706..41272
                     /gene="mepS"
                     /locus_tag="RH058_000836"
     CDS             40706..41272
                     /gene="mepS"
                     /locus_tag="RH058_000836"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV3136462.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41453..43009
                     /locus_tag="RH058_000837"
     CDS             41453..43009
                     /locus_tag="RH058_000837"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV3136463.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            43091..44905
                     /locus_tag="RH058_000838"
     CDS             43091..44905
                     /locus_tag="RH058_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3136464.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPEAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44906..46000
                     /locus_tag="RH058_000839"
     CDS             44906..46000
                     /locus_tag="RH058_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV3136465.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QQITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            46000..47025
                     /locus_tag="RH058_000840"
     CDS             46000..47025
                     /locus_tag="RH058_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3136466.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVIGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            47027..48616
                     /gene="yejF"
                     /locus_tag="RH058_000841"
     CDS             47027..48616
                     /gene="yejF"
                     /locus_tag="RH058_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV3136467.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(48620..48964)
                     /locus_tag="RH058_000842"
     CDS             complement(48620..48964)
                     /locus_tag="RH058_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136468.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT        12041 a        12772 c        12084 g        12280 t
ORIGIN      
        1 tatcccttac ggctaccggg tcgggcgcga ggtaacatcc ctgtaaaacg cgccctcagc
       61 ccacatccat gtgggctgcc ccggccttca gggaacgcct cggcaatttt gacgccacca
      121 aacaaccgtg cggcctattg ataaagagct aacacattgt caaaaaacat cactatggtt
      181 ttttagagtt tctcgatatc aattgcctga atagcccttg caatatcagg ggaattattc
      241 aacacccgaa catgctgaaa taatcccgtt gcttcatcgt attctttacg caaataactc
      301 aaccactgtt taatccgcgc aacgtgatat aacccggtat cgccctgctt ttccagacgg
      361 gtatattttt gcagcaaagc aaccacctcc ggccacggca ttcgcggttc gttatatttt
      421 actacccggc tcaggttggg aatattgagc gccccgcgac caatcatcac tgcgtcgcag
      481 ccgctgatcg caatgcattg ttgcgcgctc tgccagtccc agatttcgcc gttggcaatc
      541 accggaatat tcagccgctg gcgaatctcg ccaatcgcct gccagtcaat atgctccgcg
      601 cggtaaccct gctcttttgt ccgtccatgc accaccagct ccgtagcacc agcctgttga
      661 accgcatcgg cgatttcaaa tttcttctca ccgctgtccc agcccagacg cactttcacg
      721 ctgacgggca aatgcgccgg tacagcttca cgcatcgctt ttgcaccctg gtagatgagt
      781 tcaggatctt tgagtaacgt cgccccacca ccgctaccgt tgaccgtttt cgacgggcag
      841 ccgcaattga gatccacgcc ccaggaacct aattccaccg cacgggcggc gttctctgcc
      901 agccattgtg ggaactgacc caacaactgc acgcgcacca gcgtaccgga tggtgtccgg
      961 ctggcgtttt gtagctcagg gcaaatgcga tgaaaaactt ttaccggcag cagttgatcc
     1021 accacgcgga caaactcggt gatgcacaga tcgtagtcgt taacttcggt cagcaattca
     1081 cgcaccagag agtcaagcac tccctccatc ggtgccagta acacacgcat atcatcaccc
     1141 gcaaaataat gaggcgctat gttagcgcct atggtcagcg gattaaaggt ctacaaagag
     1201 ggattgctgc aaactgcaat ccctctctat gcttgatgta tgcctgagtt cgttgcgcat
     1261 tgcgtaatgc cggggagcga aaaaataagc gctgatttct taatgtgatc ggtagcacgt
     1321 tttacgaatt aattgtatga tgaatccatc tcatctgggg tgttgattat gagtaagaca
     1381 ctgaacatta tctggcaata tttacgcgct ttcgtcctga tttatgcctg cctgtatgca
     1441 ggcattttca ttgcttccct gctaccggta accattccgg gcagcatcat cgggatgctg
     1501 atcctgtttg tcctgctggc cttgcaaatt cttccggcga aatgggtcaa tccggggtgc
     1561 tacgtactga ttcgctatat ggcgctattg tttgtgccga ttggcgtagg cgtcatgcaa
     1621 tattttgatt tgctccgcgc gcagtttggc ccggtagtgg tttcctgtgc tgtcagtacg
     1681 ctggtggttt ttctggtggt gagctggagt tcacaactgg tacacggtga acgtaaagtc
     1741 gtaggtcaga aaggatcaga agaatgatgg cgaatatctg gtggtcatta ccgctgacgt
     1801 tgattgtctt ttttgccgcc cgcaaactgg cggcacggta taagtttccc ctgcttaacc
     1861 cgttgctggt agcaatggtg gtgatcattc cttttttaat gctgaccggc atctcttacg
     1921 acagctactt taaaggtagc gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc
     1981 tggcctatcc tttatatgag cagctacacc agatccgcgc gcgctggaaa tcgatcatca
     2041 ccatctgttt tatcggcagc gtggttgcga tggtgacggg gacttccgtg gcattgttga
     2101 tgggcgcttc accggaaatc gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg
     2161 caatggcggt tggcggcagt attggcggta ttccggcaat cagcgcagtt tgcgtgattt
     2221 tcgtcggcat cctcggcgcg gtatttggcc atacattgct taatgcgatg cgtattcgta
     2281 ccaaagctgc gcgcggtctg gcaatgggga ctgcctcgca cgccctcggt acggcgcgct
     2341 gcgccgagct ggattatcag gaaggtgcat ttagttcgct agcgctggtg ttatgcggga
     2401 taattacttc gctgatcgca ccgttccttt tcccgattat tctggcagta atgggctaaa
     2461 atttgcgatg cgtcgcgcat ttttgatgta agtttcacgc gttgcataat taatgagatt
     2521 cagatcacat ataaagccat aacgggttcg taaactgtta tcccattaca tgattatgag
     2581 gcaacgccat gcatccacgt tttcaaaccg cttttgccca acttgcggat aacttgcaat
     2641 ctgcactgga accaattctg gcagacaagt acttccccgc tttgttgacc ggggagcaag
     2701 tctcatcgct gaagagcgca acgggactgg acgaagacgc gctggcattc gcactacttc
     2761 cgctggcggc ggcctgtgcg cgtacgccat tgtcgaattt taatgttggc gcaattgcgc
     2821 gcggtgtgag cggaacctgg tatttcggtg ccaatatgga atttattggt gcgacaatgc
     2881 agcaaaccgt tcatgccgaa caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag
     2941 cgcttgcagc catcaccgtt aactacacgc cttgtggtca ctgccgtcag tttatgaatg
     3001 aactgaacag cggtctggat ctgcgtattc atttgccggg ccgcgaggca cacgcgctgc
     3061 gtgactatct gccggatgcc tttgggccga aagatctgga gattaaaacg ctgctgatgg
     3121 acgagcagga tcacggctat gcgctgactg gtgatgcgct ttctcaggcg gcgattgcag
     3181 cggcaaaccg ttcgcacatg ccttacagta agtcgccaag cggtgtcgcg ctggaatgta
     3241 aagacggtcg tattttcagc ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc
     3301 caccgttgca gggagcgtta attctattga atctcaaggg ttatgattac ccggatatcc
     3361 agcgcgcggt tctggcagaa aaagccgatg cgccgttgat tcagtgggat gccacctccg
     3421 caacgctgaa agctctcggc tgtcacagta tcgaccgcgt acttctcgct taagcctggt
     3481 gccggatgcg gcgtgaacgc cttgtccggc ttgccagccc tctcctggtg tcgaaattcc
     3541 cggcaaacag tttgccgttt cttgcgcaaa accagcgggt aaagtagcct gatggaaatt
     3601 ttccttagat cgagtctcct gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat
     3661 cggcttgctg ctgttgactg tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc
     3721 ttatatctac gacgaattgc aggatctccc ctaccgccag gtcggcgtgg tgctcggaac
     3781 agcaaaatat tatcgtactg gcgtaattaa tcagtattat cgctaccgca ttcaaggagc
     3841 gattaatgcc tataacagcg gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt
     3901 gcaaagttat aatgagccga tgaccatgcg caaagattta atcgctgctg gtgtcgaccc
     3961 aacagatatt gttctcgatt acgcaggctt tcgtacgctg gattccatcg tgcgtacacg
     4021 caaagttttc gatactaatg atttcattat tatcacccaa cgtttccact gtgagcgggc
     4081 attatttatt gcgctgcata tggggattca ggctcagtgt tatgccgtac cgtcaccgaa
     4141 agatatgctg tcagtacgta ttcgcgaatt tgccgcccgt ttcggtgcgc tggctgacct
     4201 ttatattttt aaacgtgaac cgcgtttttt agggccgctg gtccctattc cggctatgca
     4261 ccaggtgccg gaagatgcgc aggggtatcc cgccgtcaca cccgaacagt tacttgaatt
     4321 acaaaagaaa caaggaaagt agttatggat gtacagcagt tttttgtcgt tgccgttttt
     4381 ttccttattc cgatattttg tttccgcgaa gcatggaaag gctggcgcgc aggcgcgatt
     4441 gataaacggg ttaaaaatgc accggaaccg gtgtatatct ggcgagcaaa aaatcccgga
     4501 ctctttttcg cttatatggt ggcatatatc ggcttcggaa ttttatctat cggcatgatt
     4561 gtttatctta ttttctatcg ttaatatttt ctcttttctg ttaattattg agaataatta
     4621 ttacttcact caataagctg cggatataac attctgaacc gcagctattt gtgaatcctt
     4681 tcacagtata aatttcccca cgcgcatagc cttaatatca gcacgcaata tttatactct
     4741 aaataattcg agttgcagga aggcgacaag tgagtgagtc cccaggagct tacataagta
     4801 agtgactggg gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat
     4861 ttagcgttcg ccttaattac tcatagcatt aaggaagatc acatgccgca acaaaattat
     4921 ctggatgaac tcactccggc ttttacgcct ttactggcga ttaaagaagc ctctcgctgt
     4981 ttattatgtc atgacgctcc ctgtagtcag gcttgcccgg cacagaccga tccggggaaa
     5041 tttattcgct caatctactt tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat
     5101 aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg agaaattatg tcaaagcggt
     5161 tgtacccgtg ccggtgttga tactcccatt gatatcggtc gcttacagcg ttttgttact
     5221 gattttgaac aacaaactgg gatggaaatt tatcagcccg gtactaaaac gctcggcaaa
     5281 gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca gtgtaacact gacaaaccag
     5341 ggttatgacg tcacgattta tgagaaagaa gcgcaccccg gtggctggtt gcgtaacggt
     5401 attccgcaat tccggttacc gcaatcagtg ctggatgcag agatcgcccg tattgaaaaa
     5461 atgggcgtga ccattaagtg caacaacgaa gtcggtaaca cactcaccct tgagcagctg
     5521 aaagcagaaa accgcgcggt actggtcacc gtggggttat caagcggttc cgggctaccg
     5581 ctgtttgagc atagtgacgt tgagattgcc gtcgacttcc tgcaacgtgt acgacaggcg
     5641 caaggcgata tcagcattcc acaaagcgca ttaattatcg gcggcggtga tgtcgcgatg
     5701 gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg taacctgcgt agcgcgtgaa
     5761 gagttagatg agtttccggc aagcgaaaaa gagtttgcca gcgcccggga gctgggcgtt
     5821 tcgatcatcg atggattcac gccagtagcc gtggaaggca ataaagtcac gtttaagcat
     5881 gtacggttac cgggcgaact gacgatggcg gcagataaaa ttattctcgc cgtcggtcag
     5941 catgccagac tggatgcctt tgcggagtta gagccgcagc gtaacaccat caaaacacaa
     6001 aattatcaga cccgcgatcc gcaagtcttt gctgctggcg atattgttga gggtgacaaa
     6061 accgtggtct atgccgtgaa aaccgggaaa gaagccgccg aggcgattca tcactattta
     6121 gagggagctt gctcatgtta acgaaagatc tttcgattac tttttgcggc gtgaagtttc
     6181 ccaacccgtt ctgcctctct tcttcgcctg taggcaactg ctatgagatg tgtgccaaag
     6241 cctacgacac aggttggggc ggcgtggtgt ttaaaacgat cggctttttt atcgccaacg
     6301 aagtctcgcc gcgttttgat catctggtga aagaagatac cggttttatc ggcttcaaaa
     6361 atatggagca gattgctgaa catccgttgg aagagaatct ggccgccctg cgtcagctga
     6421 aggaagatta cccggacaaa gtattgatcg cttcgatcat gggggaaaat gagcagcaat
     6481 gggaggagct ggcgcgcctg gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct
     6541 cctgtccgca aatgacttct catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg
     6601 tagaaaaata ttgtcgggca gtgaaacggg gttccacgct gccaatgctg gcgaagatga
     6661 cgccgaatat cggtgatatg tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg
     6721 gcattgcggc gattaacacc gttaaatcca tcaccaatat cgatcttaat cagaaaatcg
     6781 gtatgccgat cgttaacgga aaatcgagta tttccggata ttccggtaaa gcggtaaaac
     6841 cgatcgccct gcgcttcatt cagcagatgc gcacccatcc agaactgtgc gatttcccaa
     6901 tcagcggtat cggcggcatt gaaacctggg aggatgcggc tgagttttta ttgctcggcg
     6961 cagcaacgtt acaggtgacc accggcatca tgcagtacgg gtatcggata gtggaagata
     7021 tggcaagcgg gttgtcgcat tatctcgccg atcagggatt tgattcgctg caggagatgg
     7081 taggtctggc gaataacaat attgtcccgg cggaagattt agaccgcagt tatattgtct
     7141 atccccgtat caatcttgat aaatgtgttg gctgtggacg ctgttatatt tcctgttacg
     7201 acggcggtca ccaggcgatg gaatggagcg agaaaacccg cacaccgcat tgtaataccg
     7261 agaaatgtgt gggttgtctg ctttgtggtc acgtctgccc ggtgggttgt attgagctcg
     7321 gggaagtgaa gtttaagaaa ggcgagaaag aacacccggt aacgttgtaa aaccagagac
     7381 gcatccggca tttggcaccg attgccggat gcagcgcatc cggcaatatc cacactcaca
     7441 tcacttctta cgcgcgtatt tcagtgaatc cagcgctacg gcgaagataa taatcgcccc
     7501 tttgatgatg tactgccagt atgggtttac gccgatatac gtcaggccat agttgatgac
     7561 ggtaaaaata attaccccgg tcaccacgcc aatcaccgtc cccacaccgc cgctgaacga
     7621 tacaccgcct accacgcacg ccgcgatagc atccagctca tacataaagc cgaggttgtt
     7681 ggtggcagag ccgatacgtc cggcttctaa catcccgcca aaggcataga acacgccaga
     7741 caacgcgtag atcatcagca ggttcaggcc gacgttgaca ccagatactt ttgccgcttc
     7801 cgggttaccg ccaatggcaa aaatgttctt accgaagcgg gttttgttcc acaacaccca
     7861 gacgaacgcc accgcaatca acgcgtagaa ggtgatgtaa gagagacgga aactccccag
     7921 cgcgacaaag ccctgagcaa aggtagagaa gccactgtca aaaccagaaa ttggcgacgc
     7981 cccgacaaag tcgtaataga gcgagttgat gccatagacg atgatcatcg tgccgagcgt
     8041 ggtaatgaac ggcgtcacgt tgagataagc gataatcaga ccgttgatca aaccgatcac
     8101 cgcaccaatg gcacagacaa tcagaataac cagcgcaatc ggcatcgtcg ccatttccgg
     8161 gaacactttg ttggcgttat ccatggactg caataatgtc gcagccacca ctgccgccag
     8221 ccctacctga cgaccagcag aaagatcggt cccctgggtg acaattaacc ctgccacacc
     8281 gagcgcgata ataatacgca ccgatgactg ggtgagaata ttacttaagt tcaacagact
     8341 taaaaatgtt gggtcctgga aaataataat cgccagcaaa actaaaagaa cgacgtaaat
     8401 accgccctct ttcaggtaag taagaaaact tttcttattt aacgcactca tgggaagccc
     8461 ctaatcttaa aggtgcaaag acgcaagacg cagaatttcg ttttgcgttg ttgtttttgt
     8521 atcgacaatt ccggaaacga gaccattgct catgaccaga atacggtctg ttatccctaa
     8581 caactcaggc atttcagagg agataataat aatccccttg cctttcttcg ccagttcggc
     8641 aattaactga taaatttcaa acttcgcccc gacatcaata ccgcgcgtcg gttcatcgag
     8701 cattaatatt tctggttgcg ttagtagcca gcgaccaata atcacctttt gctgattacc
     8761 accggagagc gaaccaattt gcgtccgatg acccggcgtt tttacccgca tcgaatcaat
     8821 cacccactgg gtatcgcttt tcatccgcga gttatccagt aaaccaactt tatttttgta
     8881 gttgcgaata ttggaaatta acgagttaaa accaatatcc agataggcat aaattcccgt
     8941 tgagcggcgc tcctcagtta ccagtgcaaa tccgtggttt atggcttcgt tggcattatg
     9001 gttattgatc tgtttgccgt gcaacgtaat ggtgccagcc gatttctcgc gaataccaaa
     9061 taacgtctca acaatatcgg tacgtttcgc ccccaccagc ccggcaatac cgaggatctc
     9121 ccctttatgc agatcaaacg agacatcgcg aatcgacggc tggcgcagtg acgttaggtt
     9181 acgcacctcg aggatgactt cgcccggctt gttttctttg tcaggaaaac gctgattaag
     9241 agaacgccca accatcatgg cgatgatctt gtccatcgtc agtcctgcca gcggttcggt
     9301 ggcgatccac tgaccgtcgc gcaatacggt aacttcatca cataactgga agatttcttc
     9361 cattttatgc gagatataaa caataccgca gccgcgctct tttaatttac gaataatagt
     9421 gaacagatga ttgacctctt tttcggttaa cgaagaagtc ggttcatcca taatcacaat
     9481 tttcgcgtta taggaaaacg ctttggcgat ttcgatcatc tgcatttgcg aaacggataa
     9541 tgtgccgacg cgcgcacgcg gatcgatatc aatatccagt tcatcaaaaa tcgctttggt
     9601 ttcgcggtac attttgtcct gatcgacaaa catgcctttg gtgggatatc gccccagcca
     9661 catgttatcc atcaccgaac gttgtaatac caggtttaac tcctggtgta ccatcgaaat
     9721 accattttcc agtgcttctt tggcagaatg gaaatcgatc tctttaccct ggaataaaat
     9781 ggtgccggag tctttttgat aaataccaaa caggcatttt aataatgtcg attttcctgc
     9841 accgttttcc cccattaatg catggataga atgtggccgg acttttaaat taacgttatc
     9901 aagtgcctta acaccaggaa aggacttgtt gataccgctc atttccaaca agtattcccc
     9961 ggaagacgga gtcgttgagc tgaccatata attttacctt gttggccata caataagggc
    10021 gcagtaaacg actgcgccca atcagtctta tttcttgctg aattcagcca ggttgtcttt
    10081 atctacgcca acataaggta cgcggaccac tttgttgtcg attttccagt tggtgccatc
    10141 agccgcacct ttgccatcgg ccaggttttt cgccagatca aaggtcgctt tcgcctggtt
    10201 gttagcatcg ttcagtactg tgcccgccag tgcaccggat ttcaccagcg ccagcgcttc
    10261 tggcagcgca tcgacgccaa acaccggaat gctggacttg ttgtgtgctt tcagtgcttc
    10321 taccgcgccc attgccatcg catcgttgtt ggcgataacc acttcgattt tgttggcgtt
    10381 cgggccagac agccaggcgt ccatcttatc tttcgcctga gcggtatccc acatagcggt
    10441 atctaactgt aactgttcag ttttaatgcc tttgtcgttc agctctttaa tcacgtaagt
    10501 ggtacgtgct tctgcatccg gatggcccgg ttcacctttc agcaatacga attgaatctg
    10561 accgtctttg ttcagatccc aaccctgatt cgccgcccag tgtttagcaa tcaaatcgcc
    10621 ctgaataatg ccggactctt tggaatcagt gccaacgtag taggctttgt cgtagctatc
    10681 cagcgcctta cgagacggtt ctttgttgaa gaaaaccacc ggcacgtttt gcccacgcgc
    10741 tttctcaatc accgtacccg cagctgccgg gtcaaccagg ttgattgcca gtgccttcac
    10801 ccctttcgcc agcaatacgt cgatctgatc gttctgcttg gactggtcat tctgagaatc
    10861 attcatcagc agctgaacat ctggcgcggc tttcgcatct tgctcaatag ccttgcgcac
    10921 tacagacata aagttatcgt cgtacttata gattgttaca ccaatgcgag tatcagcagc
    10981 gtgtgcagcg gcaccgaata acatgctggc catcacagca gacagggtta acaccttctt
    11041 attcatggta tctccggttt ttcttatgca gggtagtgct tgagataaat gctcggcggg
    11101 gcagtagagt taatgaagtg ttactgaacg ccgaagctca ctttttaaaa ttcgttcttc
    11161 catgcccggt aacgctccag aaaacggctt taattgttgt tatgacgcta ttacctcggt
    11221 gaaagtgatt attcactgtt acatacgggt tacaacgtta aaacggtgca atcatagcta
    11281 tcacattgtt aagatactgt gaaatcactc acagattgaa agcggttaca tcgcctgatt
    11341 tgttgagtta gtgatcgtcg ccgcattctg gcgtgttgcg acagaatggc gacgcactaa
    11401 cgtcggcata aaacagtggc tggcacgagg atcaatattg cctgctgccc cctgcaaggc
    11461 cagttcggtg gctaatttcg ccattgaagc aatgggataa cgcacggtcg ttaattgcgg
    11521 gtcggtgtaa cgggcgatgg gaatatcatc gaaaccgatg attgagagat gtaacggaat
    11581 cgcaatgcca ttatctttta atgctgtcag tgcgccagcg gccatattgt cgttataagc
    11641 aaatacagcg gtaagttgta gattgcgccc cagcagttca accatcgccg cctcaccgcc
    11701 cggcatgtcc ggcgtaccag tgccaatcca gctttccggc ggaataatat cctgctcttt
    11761 caacgcactc atccagcctg ctttacgcat ggcgtcatct tcaatgccgt ggctggaaga
    11821 aagataacca atacgttgat gaccgttatt cagcaacatg cgcgtcgcca ttcgggcacc
    11881 gctgagatta tccaggcaaa cgcaacgatg ggcgtacccc ggcacaacgc ggttgattaa
    11941 caccataccg ggaatgttat ccataaattg cgccagttca tcgtcactca atgcttttga
    12001 gtgaacaatc aacgcattac aacgctggcg aattaacacc tcaatggcgt gacgctcttt
    12061 ttccgcttca tgatagctat tgccgattag cacgtatttc tgatgctgct gggcgaccag
    12121 atccaccgct tttaccagcg cgccgaaaaa cgcatcagaa acgtccatca ccaccacgcc
    12181 aatggtatcg ctaacctgag ttgccagcgc ctgggcattg gcatttggcc gataatccag
    12241 ctcactcacg gctttcatta ctgcttcacg cgtgtcggca ctgaccagcg tgctgttatt
    12301 gagcacccgg gaaaccgttg ccacagagac gccagcctga cgcgctacat cacgaatggt
    12361 gatcatattc actacctgtt atgagattgc agtaaatgac tgcttgctgg cggctatttt
    12421 gtcagcactg aatacaggac ttcgtgaatc gagtcacagc aatggaaacg gttacagccg
    12481 ttttgttaac gaatgtgatc cagatcgtta tcttgatgtt ttagatattg ccgttccggc
    12541 agcacgcaaa gttaactgac gccagagcca ttccaccggc ccctggcgga agtaacgcag
    12601 ccagataaca gagaagagga tattcgccag ccataccgga ataacaaacg ccagcagctc
    12661 caggcgatca aaatgcataa acaaaccgag gtggtaaaaa agcgtggtac aaatcagcgt
    12721 ttgcaataga tagttggtca gcgccatccg tccgacgcag gcgattgcca gcaccagctt
    12781 aaagcggctt aattgcggcc agaagccata aaacagcgac gcatagccaa tcgcctgaaa
    12841 cggcgcactc agttcccgcg gcatttgaag taagaaggca caccagcgat acgcccagtc
    12901 cagctgccat tgcagggcga tggcaggaag gttaatggtc accccaatcg ccaccagcac
    12961 aaaaccagta cgacgatagt gacgtaagct gaactgccct ttcagccagc cgctgcgcat
    13021 cagcgccgca ccaatgagca tcatcccagc cagttgccag ccatactgtg cgcccagtgc
    13081 cagtaaactg ttgccaacac catcggcacg attactgatc gcatcaacgc cgccgtgaag
    13141 cttccagtat ttttcataca aaatagccga tgcatccggc gtccaggcgc ggctggtctg
    13201 gctgtcggaa atcagcccca ataacagcaa aacgccaagc cccaccagat aaagcatgac
    13261 gcctgtatta aacaggcttt ttaccgatgg cgcatcgcgc accagccgcc agcagattaa
    13321 gcccaccagc ccgtaagcca gcagaatatc gccgtcccag aacaataaac cgtgaataaa
    13381 gcccaataag actaacagcg ttaaccgcga ctggatccag cgtctgccac ggggcagcaa
    13441 catttgcagt ccagcaccaa acagcagcgc aaaaagcgtg aggaatttca cctggccgat
    13501 gagatcaaga aatgcccagg tccatgcatc ctgcggcgta atagcaccgt accaggcggg
    13561 attgagataa gccgcctttg gtagcccaaa ggcgctgatg tttaatagca ggatccccag
    13621 aatggcgacg ccgcgaacaa aatcgagcgt gacgttgcgc tccatggttc ctgcctttta
    13681 atcagttgtg atgacgcaca gcgcgcagaa actcgtggcg cgtattctga ctggatttga
    13741 acaatccacc aagagaggtc gttgttgtgg cactggttgc atcgcggatg ccacgcgcct
    13801 tcacgcagta atgcaccgcg tcgatcgaga cagccacgtt attggtgccc agcagcgttt
    13861 gtagcgcaat aagaatttgc tgcgtcagac gttcctgcac ctgcggacgc tgggcaaaga
    13921 actgcacgat acggttaatt tttgacagac cgatcaccga atctttcggg atataagcca
    13981 ccgtcgcttt gccatcgatg gtaacaaaat ggtgttcaca ggtactggtc agagtgatat
    14041 cgcgcacggt gaccatttca tcgaccttca ttttgttttc aatgagggtg attttcggga
    14101 agttggcgta atccagaccg gagaaaattt catcgacata cattttagcg atgcgatgcg
    14161 gcgtttccat caaactgtca tcagccaggt cgagattcag cagctgcatg atttcggtca
    14221 tatgaccagc aataaggctt ttgcgcgttt cgttatccat ttcatgcacg ggcgggcgca
    14281 gcggtgtttc cagtcctcgc gcaactaacg cttcatgaac cagggccgct tctttactga
    14341 gtgatggcat ttatgatttc tcctgcaggt gtgacgcctc cgccctgcgt gggggcaaag
    14401 ttattaagtt gatttacagc ctgattattg tgcgtgaggc ggcgcacata atccagtatt
    14461 cacagcgata attattgtaa ttgccgctgc ctttcatcag cagatgttaa aacatcgtta
    14521 tgcaaatacg gaagtgaaag ttactcacag cacattgaat aaacggtatg atgaagaaat
    14581 tgcaaacaac acaacaagga gccacgcatg gaaatgctcg aagagcaccg ctgttttgaa
    14641 ggctggcagc aacgctggcg acacgactcc agtaccttaa actgcccgat gacgttcagt
    14701 atctttctcc caccccctcg tgatcacacc ccgccaccag tgctgtactg gctttccgga
    14761 ttaacctgca atgacgagaa cttcaccacc aaggcgggtg cccagcgcgt ggcggcagaa
    14821 ctggggattg tactggtgat gccagacacc agcccgcgcg gcgaaaaggt tgccaacgac
    14881 gatggctacg atttaggcca gggcgcaggc ttttatctta atgccacgca accgccgtgg
    14941 gcgacgcatt accggatgta tgattatctg cgcgatgaat taccggcgct ggttcagtcg
    15001 caatttaatg tcagcgaccg ctgcgccatt agcggtcact caatgggtgg tcacggtgcg
    15061 ctgattatgg cgctgaaaaa tccgggtaaa tacaccagcg tttcggcctt tgcgccaatt
    15121 gtgaatccgt gcagcgtccc gtggggaatc aaagcgttta gcagctattt aggtgaggac
    15181 aaaaatgcat ggctggaatg ggacagttgc gcgctgatgt atgccagtaa cgcgcaggat
    15241 gcgatcccga cgcttatcga tcagggcgat aatgatcagt ttcttgccga ccagttgcaa
    15301 cctgcggtac tggcagaagc cgcgcgccag aaagcgtggc cgatgacgct gcgtattcag
    15361 ccgggatatg atcacagtta ctacttcatc gcctctttta tagaggatca cctgcgcttc
    15421 catgcgcagt atttactgaa gtgaaagtcc gcccggttcg ccgggcatct tctcatcaga
    15481 agcgataatc cactgccata aagtaacgac gtccgtcttc gttatagctg tagtcgtcac
    15541 gactgagatc cttgtcgcca aggttcagca cgcctgcgcg cagtttgacg tctttagtca
    15601 cctgccaggc cgcgccggta ttccagatgg tataaccgcc cggtgtttta gccgtcgcgc
    15661 tgtcggcgcg tttctgcccg gtatagtgac cagaaacata gaatgaccag tcttccagcg
    15721 ccagcggttt ccagtccagc gtaccgttag cagtatggaa cggcagatcg gatagcggtt
    15781 tgttttcgcc gttgctgaca tcacgaccat cgttgtaggt gtagttgatc gacagtttcc
    15841 attcatcgtt gaacggaatt ttcagttcgg tttccacgcc ctgaatacga gctttgttaa
    15901 cgttgtagta gctaaatacc ggtatgcgcc gtccgttagc gcccgtctca aaaccaacaa
    15961 agttttggta gcccggtgca gcgttgacgt cagacgtacg gctgatgctg atacgatctt
    16021 tcacatcgtt acggaaaacg gtaacgctgg attcaacccc ttccagccag ccttcttcac
    16081 ccatgtagta aagccccagc tcccaacttt cgctggtttc tggtttcaga tccgggctac
    16141 ccacaatctt acatgcgcca cggcaggaat tgctcgtcca gtcagggcta agttgcaaca
    16201 gagaaggtgc tttaaatgcc gtcgcccagc cccctttcac cgttacggtg tcggtggcgt
    16261 tataaaccag gtaggcacgc ggactccagt gttcaccgta ggtttcgtga tcgtccatac
    16321 gcacgccggt cgtcagcgcc agcggctcga agatccgcca ttcatcttcc acaaacagcg
    16381 cgtactggct ggcagacgtt ttggagctgg ttcccccggt caggttcacc gcatcgctaa
    16441 gtttgtcgtg acgccattca ccgccaaccg tgagaaactg attaatcgcc gtcagcggca
    16501 acgtgtattt gccgtcgacc gtattgcttt cggaagttat cgggctgctg ttgccagggt
    16561 ttttgttctc gactttctca ccgtagtatt tcagttcgct ggtgccgtaa tcccaacgcc
    16621 cattatggct gacggagtag ttctggcgtt ccaggcggtt tttgtccagc gagtcggaat
    16681 cacgatcctg acggtcgaaa ccgtatccgg cagtaaaatc gtgattttga ttcggtgtcc
    16741 aggcaaattc gacattgccg tcgcggctgg agaatccttc aatacgcggc gtttctccgg
    16801 tatcggtggt cgttgagttt tgcgggtcat ccttttcacg ttttgccagg ctgccgtaag
    16861 ctttcattcc cagcacacca tcaattaatg gtccactggt aaagaactga ccgttatagg
    16921 tatcaccgcg atcgcgatgt tcctgaatgg tggtatcgac ggtaacggta cccgaccatt
    16981 tctgaccgat ttttttggtg atgatattca ctacaccgcc gagcgcatcg gagccgtata
    17041 gcgacgacat cgggccacga accacttcaa tacgttcgat ggaatcgacc gggatccagt
    17101 tcagatcgaa atcattgtgg cggaagacgg cattgcggga gttaacgcgt ttaccgtcga
    17161 cgagaatcag ggtgtagctg ctgtccagac cacgaatact aacgccctta cggttatccc
    17221 ctgttaacgc gtttaccgtc gacgagaatc agggtgtagc tgctgtccag accacgaata
    17281 ctaacgccct tacggttatc cccttcgttc gtcagttgta cgccaggcac ttctttgagg
    17341 acatccttca gattctgtac cggttttcgc tgcaggtctt cctgggtaat gacgctgata
    17401 ctggcgggtg catctttgag gttttgttca acggaagatg cagtgacaac catcgtttcg
    17461 ccatcatcat cgaccgctaa cacaggccat gcacaagaaa tagcggacaa acacagcccg
    17521 acccgtacga aagggttcaa cctaaacatt ccatatctcc atgaggtaac tacgaaaata
    17581 aaatgggtta tcgctcacat cttcttcacg tccccttgcg tacggcagca tcgcggtgga
    17641 cttatttgta tgcaggtgat catcccagaa agccggaaca gcctctgatt tgtcagcctt
    17701 ttttgattgc ggcgtaacga taatgcaaac gataacaatt atcaatccaa atgttaaatt
    17761 ttatatccgc atgatttgtc agggaaatgg aattgaaaaa gccctctcgg ttgagagggc
    17821 ttagcaagga agggaggaaa cttatttctt atcgttctgc gggaacttca tttcgctgta
    17881 acgtacgaag tgagttcctt taatcagctt gtagccgaac caaataatca ggaacagcgg
    17941 gataccaata tacgttgccg ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc
    18001 gtagttctgg cccaaagtga taatcagaca cagaatgaat gcgaagatcg gccccagtgg
    18061 gaagaaacct gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata cgtaaccgcg
    18121 acggaagcga tagtggctaa tggcaatccc cagccaggcg ataaaacccg tcatcccgga
    18181 ggtgttcagc agccacaggt ataccgtctg gttgccaaac atggaggtca ggaagcacag
    18241 accggcaatc accgtcgtcg catacagcgc attacgcggc acgccaccac gcgacagttt
    18301 agcgaaaatg cgcggcgctt taccgtcaca cgccagggtg tacagcatac gagtagacgc
    18361 atacataccg gagttacccg ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc
    18421 cgccgcagag agcagacccg cgtgctggaa caccagggtg aacggactaa cgctgatgtc
    18481 tttaacatcg ttacgcagca ggctcggatc ggtgtacgga ataatcaggc tgataatcag
    18541 gatcgcgaac acatagaaca acaggattcg ccagaacacc tgacgtaccg cgcgtggaat
    18601 gtttttcgcc ggatcttcgg actcgcctgc agcaataccg atcagctcgg ttccctggaa
    18661 agagaagccg acaatcatag ctacgccgat catcgccgca aaaccaccag caaacggcgc
    18721 ttcgccgatt gtccagttgc tccagcccgc aggctgcgcg cctttgaaga taccgataat
    18781 catcagcacg ccaacgatga taaagacaat aactgtcgtg actttgatca gtgagaacca
    18841 gtattccgct tcaccaaagc cacgaactga gatgtagttc agcaggaaga taacgccgag
    18901 gaacaacgca ctccagatcc agcccggtgt atccgggaac caccagctca tgaccagctg
    18961 agctgcaacc aggtcaacgg cgatagtcac cgcccagttg taccagtagt tccagcccag
    19021 cgcgaagcca aagccttctt caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac
    19081 cggcatatat gcagccagtt caccgagact ggtcatcagg aagtaaacca tcaggccaat
    19141 cagcatatac gagagcaatg ccccgcccgg acctgcctga gaaatcgttg cgccagaggc
    19201 aacaaaaaga cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt
    19261 taattcacgg cgtaagcccg gcgcttctgt ggttttagtt tcggaaacca tacaaaaatg
    19321 ctatccatct taaaaatgag gcgcgattgt agcagacgat tggcaatcct tccggcagaa
    19381 atacacggtt ataagacacc ttcatgatcg cccagggatt ataagtaaag cagccaatct
    19441 cttttctggc gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag caccagcatg
    19501 ttcttgtaca gcaacttaac gcggtacatt cgcgggatcg caatagtcca gaaagcgccg
    19561 tagcgcgttg gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac gcatcaggcg
    19621 cggcagaggg accgcaactt cacttaatgt gcctgcctgc aattgatctt caatcacccg
    19681 tcgcgacagg caactaattc ccaacccatg acgcaccgca tgtttgattg cctcggagtt
    19741 acctaattcc atcgccatct caaacttcgg taaatgtgac agcaatagat aatcgacaat
    19801 ctcccgcgtg ccggaaccgc gttcacgcag gatccacggc gcagcggcca gctgctctaa
    19861 ggtgaccgga ccacgggcca acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc
    19921 cagccacggt tcagaaataa tttcagtgct gtggcacggt ccttcaataa aaccaatatc
    19981 aacgcggaaa tccagcaccg cttgcatcac gtcctggctg ttcccaacgc taagttcaat
    20041 cggcaactgc ggataatcat ggcgataacg ggcgataact gcaggcagaa tgtagttacc
    20101 gatggtacta ctggcataga tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat
    20161 ttcaaccgcc tgttcaagca atgccaacgc acgcggatag agcagccgcc cgtgttcatt
    20221 aacaaccagt cttttcccca cgcgatcaaa cagttgcacg ccaagctgcc cttccaggtc
    20281 ggtcaaggct gcgctcactg ctgattgcga caacgccagc atcaccgacg cctgggtggt
    20341 tgatccactt ttcaatactt ctgcaaaaac ttccaactgc cggagggtga tgtgcatagt
    20401 cgcttaccac ttataaagat taattataaa tatataatca attttatttt taaaccagtt
    20461 agtcgtaacc ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac
    20521 aacatcgtac actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg
    20581 tcgccctgtg gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc
    20641 tcgcaatctt gttggggatg gttttaggca acaccatcta tccgcacatc tggaaaagct
    20701 gtgacggtgg tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg
    20761 gcttccgtct gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg
    20821 tgttgacgct gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc
    20881 tggataagca caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg
    20941 tactggcgac tgagccggta gtgaaagcgg aagccagtaa agtaaccgtg gctgttgcaa
    21001 ccgttgttat cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt
    21061 ctcaatggtt tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg
    21121 cgcaggtggt ggcggcaggt catgccatca gcccggatgc ggaaaacgca gcagttattt
    21181 ccaaaatgct gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta
    21241 aacagctgtc tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca
    21301 tcttgttcat cgtagttgcc atctttaact cgttccacct gttaccgcag agcgtggtga
    21361 acatgctggt aacgctggat accttcctgc tggcaatggc gatggcggcg cttggtctga
    21421 ccactcacgt cagcgcgctg aaaaaagccg gggcgaaacc tctgctgatg gcactggtac
    21481 tgtttgcctg gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg
    21541 cataaaccac tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg
    21601 tttaacagga gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg
    21661 gcaaatgccg caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt gttcaccaaa
    21721 aaccaacgtc agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcg
    21781 gcctgtgaaa aatatcacta cacatcggcg caaattcttc cccacgacag ctatctgatt
    21841 aacctcggac atccggtcac tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa
    21901 atgcagcgtt gcgaacaact ggggctttct ttgctcaact tccaccctgg cagccatctg
    21961 atgcagattt cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgcgctg
    22021 gataaaactc aaggtgtaac agcggtgatt gaaaacaccg ccggtcaggg cagtaactta
    22081 gggtttaaat tcgaacatct cgcggcgatt atcgacggcg tggaagataa atcccgtgtc
    22141 ggcgtctgca ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactccagcc
    22201 gaatgcgaaa aaacgttcgc tgattttgcc cgtattgtcg gctttaagta tctgcgcggg
    22261 atgcacctta acgatgcgaa aagtaccttt ggcagccgcg ttgaccgcca tcatagcctc
    22321 ggtgaaggca atatcggtca tgatgcgttc cgctggatca tgcaagacga ccgtttcgac
    22381 ggcattccgc tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg
    22441 ctgaaagcgc aacaaactga aaaagcggtg gcctgatcga tgaacgacag ggaaaagcag
    22501 attcttaaaa ttttacggcg taacccgctg attcaacaga atgaaatcgc cgatattctg
    22561 caaatcagcc gctcacgtgt tgccgctcat attatggatt tgatgcgcaa gggtttgatt
    22621 aaaggcaaag gatatatcct taccgagcag gattactgtg tggttgtggg tgcaatcaat
    22681 atggatattc gcggaatggc ggatatccgt tacccgcaag cagcctctca tccaggttct
    22741 gttcactgtt ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc tctgcttggt
    22801 cgggatgtgc atttaatatc agctattggc aacgattttt acggcgagac attactggaa
    22861 gaaacgcgcc gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg acacagcact
    22921 gcaacttatc tcgctatcgc taataagcag gaagaaacta tcctggcaat caatgacaca
    22981 catattctgc aacagctaac gcctcaactg ctcaatactt cacgggactt gattcgccat
    23041 gctggtgtgg tgctggctga ttgtaatctc acacctgaag ccctggagtg ggtctttacc
    23101 attgctgatg aaattccgat gtttgtcgat accgtttccg aatttaaagc gaacaaagta
    23161 aaaaactggt acagccggat ccacactctg aaaccaacac aaaatgaact ggaaattttg
    23221 tgggggcaac cgattaaaga cgacaacgat cgcatcagag ctgtaaattc gctgcatcaa
    23281 caaggtgtta aacgcatttt tgtttatctg aaagatgaat ctgtattttg cagcgataag
    23341 gatggagaac aatttttgct gaccgcgcca gcgcatacga cagtagacag ttttggtgct
    23401 gacgatggtt ttatggcggg cttggtatat agctttcttg aaggatacag tttccgcgac
    23461 agcgcccgtt ttgcggtagc ctgcgcggca atttcgcgcg ccagcggcag cttaaacaac
    23521 cctaccctgt ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg
    23581 attcgacgct tgaccgcatc ttatccggct cataaattgc gcactacgct aaaccaataa
    23641 agaatcctgc aatagtagca ctcatcaggt tagaaagtgt cgccgccgcc agcgcgcgta
    23701 aaccaagttg ggcgatttcc ggcgcacggt gtggcgcaac cgcagaaaac gcccccacca
    23761 ccaccccgat agaaccaaag ttagcgaaac cgcacaacgc gaaagaaata atcgccacgg
    23821 ttttagcatc cagagtgcca cccgtttgca gatatggcga gaaattgaga taagcgacaa
    23881 attcattgat cgccagcttc tgcccaatca aactcccggc aagatttgca tcactccagt
    23941 caacccccat cacccacgcc aatggggcca acaggtaacc taaaatggac tccagcgagg
    24001 catgtgcaaa accaaaccag ccgccaacgc cgccgataat accattaatt aacgcaatga
    24061 tggcgacaaa tgccataaca actgtcgcta caccggcggc gattttcagc ccggtcattg
    24121 cccctgtcgc agcggcttca ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat
    24181 taaaggaaac ctgcgaagat tccgtagccg ggcttaacag gcgggcaaac aagatcccgc
    24241 ctgggatcgc cattaacgat gccgccagca aatattccac aggtacgccc agggcggcgt
    24301 aaccaatcat tgtcgaacca gcaatcgagg ccatgccact acaaatcgct gtaaataatt
    24361 cattgcgatt cagacgatcg ataaagggtt tgacgattgc cggaatttcg ttttgcccga
    24421 ggaaaatggt agtgaccgcg acgaatgact cgatcttgct gatatttaac gctttctgga
    24481 agataccgcc gagaatgcga attaaaatcc ccatcacacc gatgtagtag agaatactca
    24541 ccagtgctgt aacgaagata attgccggta atactctgaa accaaagata aatcctgcgc
    24601 catcaaacag cgtgtccatt ttcggcccga ccagcgaacc gaagataaat gcgctacccg
    24661 cgtcgctgta cgccatcact ttatgcacgc caaaagcgac tttttcagcg acccaacgcc
    24721 ctggcggtaa ccaaagcata atgccgccaa tcacaacctg taacactaac gccgcgccaa
    24781 cggtacgcag gctgatcttc ttcttgttta ctgacagcaa aaatgcaatt gccagcaata
    24841 ccaccattcc cagaacactt ctcatgacat ccatagtgat tatctcttca tgtcatattg
    24901 ggcgacggct ttctgcccat cagaaatagc cgggcctgca tcttgcgcta tcggcccggc
    24961 ttgttatcaa cgcttaatgg gttttgatgt agccgcgcac gcactcttcg actaagcccc
    25021 agaaccagtc ggtatcaata gtgatgccga ctttggtatt ggcgggcttg ccaagaacgc
    25081 ccagctcgtc gcagacggtg cgcccatagc aagggccact gttgacgtcc acttcgacgt
    25141 acatctcctg ggttttaatg ccatcagggt taatcagata accgatgcag gtggcgtcgt
    25201 gcaccgggcc gccagccagg ccgtagtttt cgaactgcgt tttgagagtg aagttcatga
    25261 tgtcgctgaa cagctctccg gcagggccgc ctgccctttc catccgagca atcacgtccg
    25321 gggtgcaaac ggtctggttg gtgagatcga ggcccatcat cactaatgga acgccagagg
    25381 tgaacaccac gcgggctgct tccgggtcag caaagatgtt gaactcggca gatggcgtga
    25441 agttgcctgt accgtaagcg ccgcccatta gcacaatttc acggattttg ggcaggatcg
    25501 cgggttgcat acgcattgcc accgcgatat ttgaaagcgg accaaccggc accagagtga
    25561 tatcgccatc gcttgccatc agggtatcaa tgatgtattt caccgcgtgg gtactttctg
    25621 cctggcgggt cagcggctcg aataccgggc catccagtcc ggtttcaccg tgaatattat
    25681 cggcaacgat ttgttgacgc ataatgggtt gcggcatccc cgcataaacc ggaacattaa
    25741 tctccagttt ctggcaaaca ttcaggccat taattaatgt tttatcaagc gtctgattac
    25801 ccgctacaat agtgatgcct aataaatcta ttgccggatg tttcgctgcc atcatcattg
    25861 caatagcatc atcatgaccc ggatcacaat ccagaataat ttttctcttt tccattgttt
    25921 atttcctctg tttccagttg cgttattttt tctacagcaa agaaaattcg accgggccga
    25981 tgattgaatc ttaccaaccg cgtacgtatg ctaaatatga gaaatctcat agcggataaa
    26041 catcgtgaaa gaaatccaca ataatgatct taagcagcaa ttgatgagtg aatccgcgtt
    26101 taaggattgc tttttaacgg atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg
    26161 tgactacatt gtgcaggaag ggcaacagcc gtcctggctg ttttacctga cgcgaggccg
    26221 cgccaggctt tacgccacgc tagctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc
    26281 cccctgtttt attggcgaga ttgagttaat cgacaaagac catgaaccgc gtgcggtgca
    26341 ggctattgaa gagtgttggt gccttgcgct ccctatgaaa cattaccgcc cgcggttatt
    26401 aaacgacacg ctatttttac gaaaactctg cgtcacctta agtcataaaa attatcgtaa
    26461 tattgtttct ttaactcaga atcaatcatt tccgttagtg aatcgcctgg cagcatttat
    26521 attactctcg caggaaggtg atctttatca cgaaaagcat acgcaagcgg cagagtattt
    26581 aggcgtttct tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg acggtttatt
    26641 aattaaaagc aagaaagggt atctaattaa aaacagaaag cagttgtcag gactggcgct
    26701 ggagatggac ccggagaata aattctccgg gatgatgcag taaaaattat ttgcaatagc
    26761 gcgattgccg gatgcaacac ttaataagcg cgtacatccg gcaatgaaac tcaagctaaa
    26821 ccaataaaga acccggcaat ggtcgcactc atcaggttgg aaagcgttgc tgctgccagc
    26881 gcccgtaaac caagctgggc gatttccggc gcgcgttttg gcgaaatagc cgaaaatgcg
    26941 ccaacgacaa caccgataga accaaagtta gcaaaaccac aaagcgcaaa ggagataatc
    27001 gcaatggttt tcacttccag cgtgccgccc gtttgcaggt acggggagaa actcaggtaa
    27061 gcgacgaatt cgttaatcgc cagtttctgc ccaatcaggc tacccacaag attggcatca
    27121 ctccagtcca cacccatgat ccacgccagc ggtgccagca catagccaaa aatactttcc
    27181 agagaggcat tggcgaaacc aaaccagcca ccaattccgc cgataatgcc gttgatcagc
    27241 gcaataattg cgacaaacgc cattaccacc gtcgctacac cagcggcgat tttcagcccg
    27301 gtcatcgcac cgctcgccgc cgcttcgata aagctttttg gcggcgtttc gctgaacgac
    27361 agattttcaa atgtgacctg tgaaggctcg gtggccgggc taagaatacg tgcaaacaaa
    27421 ataccacctg ggatcgccat cagcgatgcc gctaacaggt agtcaattgg tacgcccatt
    27481 ccggcataac caatcatcat cgaaccagca atggacgcca tcccgctaca aattgcggta
    27541 aacaactcgt tgcgattcat gcgatcgata aacggtttaa cgatcgccgg gatctcattt
    27601 tgcccgagga aaatagttgt gactgcgaca aaagattcga ttttgctgat gttaagagct
    27661 ttctggaaaa tgctgccaag gatgcgaatc agcagcccca tcacgccaat gtagtacagc
    27721 agactgatga gcgcagtaac gaaaataatc gccggaagta cgcgaaaggc gaagataaaa
    27781 cccgcaccgt caaacaagac atccattttc ggcccaacca gcgaaccaaa aatgaaggcg
    27841 ctaccggcat cactgtaaga catcacttta tgaacgccta atgccgcctg ttctactgcc
    27901 cactttcccg gtgggaagta gagcatgatg ccaccaatag cgatttgcag cagcagtgcg
    27961 gctccaaccg tgcgcaaact gatgctcttt ttattcactg acaacagaaa tgctattgcc
    28021 agtaacacca ccatccccac aacacttctc attatatcca taatgatttt cccttcatgc
    28081 cagtaaaccc ggcgtcagcg ccaggttttg gtatgcttga tgagtacggg cgacggcttt
    28141 ctgcccgtca gaaattaacc cgcgagacgc tggtattctt tggcaatttc actcgccaga
    28201 atggcgttgt tgaacaccag ctggatattg gatttcaggc tgtcaccgcc ggtcagctca
    28261 gcaacgcgcg ccagcaggaa cggtgtactt tctttaccaa taacgccctg ctcttcggct
    28321 tcggcgacgg cttgatctat ggctgcattg atagattcct ccggcatcgc aaactgttcc
    28381 gggatcgggt tcgctaccac gaggccaccg ttcagcccgc tttgccattt caccgccatt
    28441 gcacgggcaa tttcactggc gctgtcgaga cgaatgctga cgtcaaacga gctggtacgg
    28501 cagaaaaacg caggcagcgc tttagtctga tagccaatta acggcacacc gaaggtttct
    28561 aaatactcag tggttaatcc taaatcgaga atagatttcg ccccggcaca aacaacggtg
    28621 atattagtat ttgccagttc ttgcaaatcg gcagaaatat cgaaggtatg ttccgccccg
    28681 cgatgcacac caccaattcc cccggtggca aatactttaa ttccggcaag cgccgcaata
    28741 atcatcgttg aagccacagt ggttgcgcca ttttttccgg cggcaacaac aaaaggtaaa
    28801 tcgcgacgac taactttggt cacgttatgc ccttcacgac ccagtaattc aatttcttct
    28861 ttgcttaaac ccactttcat cacgccgcca ataatggcga tagtggcagg tactgcgccc
    28921 tgtttacgaa tagtttcttc tacttcaatt gcggtctggg cattttgtgg gaacggcatc
    28981 ccgtgagaaa taatggtcga ttccagcgcc acaaccggtt ttttgttttt taaagcgtcc
    29041 tgcacttccg gggaaatttg taataattca ggggaaattt ttaattcaga cattctgcgt
    29101 tctccactaa cgatataacg ttggcaatcg ataaatcggg gttattggtg tattcacagg
    29161 agagcgccat tgacgaacat ccctgtgcga aacgaacaga ttcggcaaac ggcattccgt
    29221 ctacccaaca cgaagcaagt cccgccatca tggcatcgcc cgctccggta acattaataa
    29281 cattggtttt gatcggcgca gaccagccat tttcaccgct gatatcgctg taataaacgc
    29341 cgtcgccgcc catgctcaat accagtcggt tcaggccatg ttgatggaac caggcagcaa
    29401 cttttgccac atcgtcacgc cctgacagcg caatcccact cagggtttcc gcttcaaggc
    29461 ggtttggctt gagagtgtgg atctgattta gacggtcgcg cactttgaca catttccacg
    29521 ctgaaaccgg atcgacaaat acgggtacgt tggcggcatt atccagaatc catgccagtg
    29581 cctcttcact gatattacag tctgcgacaa tgacctttgc cctctgaata aattcacggt
    29641 gctgtgcgag atattcagct gtaatagcgt tgctaatatt catgtcattt atagcaacca
    29701 gcatttcacc ggtattatcg agtaatgata aatagcttga cgtattttct cctggcacaa
    29761 tcaggcattt atcgacataa acgccagatt gattggtttg cgttagcagc gattgaccat
    29821 aaaagtcact gcctacggcg ctcagtagcc aggctttgtt ccccagcaac gccaggtttt
    29881 gtgcaatatt gcgcccgact ccaccaggcg taaattttat tttacctgga tttgaatccg
    29941 cataatttaa tgattcatgt gaatatccgg cgacatcaat attcgccgag cctataatta
    30001 cgacataatc cttttcgcgc atagcacgtc ctctggtaaa aaattaccaa ccctgtcaat
    30061 ccacaagaat atagatactg caaagacagt ttaaacatgt gttcatattt aaacatgtgc
    30121 tcatagtatg catgttttac gtaaagtaaa ttgccgccag agaggcaaag cgggcagatt
    30181 tgagcaggct cacaaaatgc gattaaatgc cagaattaca aaagcgagga atttgtgacg
    30241 ggatgcacag aacaggaaca ctaaaaaagg gcagaaaaga atctgccctt gaggaaataa
    30301 ccttagtgtt tgatgaacgt ttcaggtttc tactgaaaga taaaaccatt taaattcata
    30361 aaattacatt cacaaaccgg tcattctctt tgcgctcatc caatgataca gggcagtaaa
    30421 tttctgccct gtaacacacc ttttattacg cggctttcgc tactgcgtcc acttccggac
    30481 gtttcaggaa ggcataggcc aaacccgcca ccagcgtacc ggcaataatt gctaccaggt
    30541 aacccaatac cggcgtaata gcgccaggga tcagcagaac aaacagacca ccgtgcggtg
    30601 ccatcagttt cgcaccaatc gccattgaga ttgcgccagt cagcgcccca cccacgatac
    30661 agcacggcag cacacgcatc ggatcacgag cagcaaacgg aattgcacct tccgaaataa
    30721 agcacagtcc cagtaccaga gcggctttgc caccttcctg ctgcgctttg tcgaatttgc
    30781 gacgcgccac cattgttgcc aggcccattg ccagcggtgg caccatacct gccgccataa
    30841 tcgccgccat cgggccatag gtttgagtac tcagcagacc cacaccgaat gcgtacgctg
    30901 ctttatttac cggaccgccc atgtcagtac acatcatgcc accgaggatc gcccccagca
    30961 gaaccgcatt cgcagtcccc atggtctgca gccagtgagt caacccttcg agaatgccag
    31021 caaccggttt accgatcagg tagatcatcg ccagaccgac caccagactg gaaattagcg
    31081 gaatgatcag gatcggtttc agcgcctcca tactctgtgg cagtttcagt tgcgtactga
    31141 ttaacttcgc aatgtaacca gccaggaagc ccgcaataat accgccaatg aagccagaac
    31201 cggtgctgac cgccagcata ccgccaatca gacccggcgt gaggcccgga cgatcggcaa
    31261 tggaaaaggc aatataacct gccagtaccg gtaccatcag cgcaaaggct gaaccaccac
    31321 caatctgcat cagcgccgct gccaacgtac ccggctcttt aaacgcttcg ataccaaaag
    31381 caaaagaaag cgcgatacac agaccaccag caacgaccat cggcagcatg taagagacgc
    31441 ccgttagcaa gtgacgataa gcgcctgcac tctctttctt accttcagtg gtcgccgttt
    31501 gagttttgcc cgccggttca tacggcgttg cttcagcaac cgctttatcc agttcctgcg
    31561 cggttttctt cagcgccaga ccggtagagg tacgatacat cggtttacca gcaaatttcg
    31621 ccagatccac ttcgatatct gccgccacaa tcaccagatc cgctgcggct acttcctcgg
    31681 gagtgattgc attacccgcg ccaacagaac cacgggtttc aactttcacc caccagccac
    31741 gttttttcgc ttcggtttca atggcttcag ccgccataaa ggtgtgtgct acgccagtcg
    31801 ggcaagcagt caccgcaact acgcgtttcg gaccgctggc ggcaaccggt gctgtcgcag
    31861 tgaccggcgc agtgtaaggt ttcgcatggc ctttggcttc actcaggaac agctcagggt
    31921 gcgcaactgc ccgggaaata tcacccagcc agacattttt accgttcagc gcgctgtcat
    31981 tcgggatgga atcaccgaga acaatcgcca tttctgcgtc gttcggattg tcgatgattt
    32041 ccagttttgc ttttcgcgcc gccgcgccca gcagggtctt cgccatatag gcgcgtgcct
    32101 gaccgagatt agcgtcaata atcagcagcg ttttcattat gcctctcctg ctgtcagtta
    32161 aaaggttgta agtcgacgcg cgccatcatt gcggccaact gcggacgatc ggtaataccc
    32221 acattgcttt gacttaccgc cagggctgca acagcagtcg ccagacgcag tgtgtgttca
    32281 ctggattcac gcatcagcaa gccataaatc aggccaccaa ccatagaatc ccctgcgcca
    32341 acggtgctta cgacatcgac tgacggtggt ttggcgatcc attcgccgga ggcattaacc
    32401 caaagcgcgc cttcggcacc cagtgaaata acaacatgcg caataccttg ttcacgtagc
    32461 gcatgtgcag cttcaatcac atctttcatt tcaggcagtt tacggcctgc ccagatttcc
    32521 aactcgcggc ggttaggttt caccagccac ggtgccgctt tcaaacctgc tactaacgct
    32581 tcacggctac tatcaaagat aatgcaagga cactgactac gcaggcgagt catccagtcg
    32641 gtgaacgctt ccgggctgac gcctgacggt aagcttccgc tgacacagac catatcgaac
    32701 tgaccgagcc agctcagaga atcagtcaca aagcgttccc agtcggcggg ggtgacttca
    32761 aaacccgaga agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt aacgttaatt
    32821 cgggtgcgcc cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct gaacagttgc
    32881 tgaaaaccat cctgattgtc tttacccagg aagccgccaa cggtgacatc aattcccagg
    32941 tcttttaata ctttggccac gttgatgcct ttacccgccg catgcagacc ggtggttttc
    33001 accaggttca cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc ataagccgga
    33061 ttaagggtga tagtagcaac acgtctgctc attatgcgcc ctccccaaga ccagcagcga
    33121 tagcgtcgcc gattgctttc agcgcctgtt cagcatctgc accctgggcg gtaaagcgta
    33181 ggcgatgacc tttcttaacg ccaagtgcca caactttcat cagactacgt ccgtttgccg
    33241 gtttgccggt accatcaagg tttgtcacgg taatatcact gttaaattgt ttaatggtat
    33301 tgaccagcat ggtacctgga cgagcatgca ggccgtgttc attgcgcacc acaaactccg
    33361 cgcttaacac gtcgtcggtc ggcgcatcat cgctggtcag cagcgccagc aacgttgccg
    33421 catccgcttt cagcaagcgg tcagctttat tgtcgagcaa taaatcagcg agacgcttaa
    33481 gaaccgcgat gggctgatcg tcattcatcg ccacactcac cagcatggct gccgtttcgc
    33541 cgtccacatc aaaagcattt gccgcacggc ttaccgcaat cgcgctacgc agattgcctt
    33601 cggcgctatc gctcagccag ataccctgtc cgagattcag cggttgttca ttgatggctt
    33661 tggtgacgaa agtggcgtca actgcccccg cctctttcag acgcgcagcg ttcagcgcct
    33721 gaagagtcag cagatcgctg gcgacgatat ccagcgtcag catttcgttg tcgagcttca
    33781 gctgctcact ctgcttttcg cccatcagta atgcgcgaag ttcttctgct gttgttgctg
    33841 acttcagttg ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg cgtagcaggc
    33901 ccagatgttc atccgagctg gcagcaatac cgattgccac gtacgctacc tgaccgtcac
    33961 cccaggtgac gccttccggg aactgaaata cctgaacgcc ggttttcagc acctgatcgc
    34021 gggtgtcggt agtgccgtgt ggaatagcaa taccattgcc gaggaacgtt gaggtttgct
    34081 gttcgcgcgc cagcatgcca ttgacgtagc cttctgctac attaccggcc tgcaccagcg
    34141 ccgcagcgac ctggcgaatc gcctcttctt tgtctccggc cttttcgccc ggatggatgt
    34201 cctgtacgga taactggaac atagttctcc tctcttgctg aattgaaacg attcagcctc
    34261 tatgagaaaa aaagcgccaa cctggcttag ggttaaagac aagatcgcgc tgaaacgttt
    34321 caagaaagca taatacttct gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa
    34381 gtttgatgtg ctgcacaatt ttttgccatt tttcctaatt gctgatggga aaatccgtta
    34441 tcagcaattt catttcagca gcgtcagcag ccaatcagca acagtgaaat ggcattttga
    34501 ttattcgtgg cgcttttgat ttcgctgtct gtttattttc tgacaagcag cgtaaactcc
    34561 gcgtcttcct cttccagtga tcgaccagca tgcataactc ccccgcagtc tccagcgcga
    34621 aatcgtttga cctgacctcg acggcgtttt taatcgttgc ctttctcacc ggtattgcgg
    34681 gcgctctgca aaccccgaca ctcagtattt ttcttactga tgaagtacat gctcgtccgg
    34741 cgatggtggg attcttcttt accggcagcg ctgtcattgg gattctggta agtcagtttc
    34801 tcgccgggcg ctctgataag cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt
    34861 taggcgtgct ggcctgcacc ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg
    34921 ttggcgtctt tcttagcagc tttggctcga ccgctaaccc gcaaatgttt gcccttgccc
    34981 gtgaacatgc cgacaaaacc ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc
    35041 aggtttcact ggcatgggtc attggcccac cgctggctta tgccttagcg atgggtttca
    35101 gctttacggt aatgtatctg agcgcagcgg tagcgtttat tgtttgcggt gtgatggtgt
    35161 ggctgttttt accgtcgatg caaaaagagc ttccgctggc gaccggcacg atcgaagcgc
    35221 cgcgccgtaa ccgtcgcgat acgctgctgc tgtttgtcat ttgtacattg atgtggggct
    35281 cgaacagcct gtacatcatc aacatgccgc tatttattat caacgaactg catcttcccg
    35341 agaaactggc cggtgtgatg atggggaccg ccgccgggct ggaaatcccg acgatgttga
    35401 ttgccggata tttcgccaaa cgtctgggta agcgtttctt aatgcgcgtt gctgccgtgg
    35461 gtggcgtctg tttttacgca ggaatgctga tggcgcattc acctgtcatt ctgttgggct
    35521 tgcagctgct aaatgctatt tttattggca ttctgggcgg catcgggatg ctctattttc
    35581 aggatctgat gcccggtcag gcgggttcag ccaccacgct ctataccaac acgtcgcgcg
    35641 tgggctggat catcgcagga tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg
    35701 ctgtgttctg gtttgcgatg gtgatgatta tcgccactct gttttgctta ctgcggatta
    35761 aagatgttta aggcgcggtc agcatttcca gatcgataag ccaggtcatg gcctgctgac
    35821 gcgagttacc gcacatttca gcgctggctt gtaagcctgc acagactttc ggtcgcagtg
    35881 gcgaggtgaa aattttacag cgttgctgtt catcaagctg aatgcagggc gtattggcgg
    35941 gcttgccatc tggcataccg ggaatagggc tggaaattga tggggcagtg caacaagccc
    36001 cgcaacccgg acggcatttc atacgttgtt ctcttaaggt taaccaccgg attgatgagc
    36061 gcgcacagta ccaccttttt tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc
    36121 cgtcgcgagt actttgtcgc gatatttttg acattttcga ctacaggaat ttttcgatgc
    36181 caagagcgaa cgaaattaaa aaaggtatgg tactgaatta caacggcaaa ctgctgctgg
    36241 ttaaggatat tgatattcag tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc
    36301 gtttttctga tgtccgtacc gggctgaaag tagaagagcg tttcaaaggt gatgatatcg
    36361 ttgacaccgt gacgctgacc cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat
    36421 atgtctttat ggataaagaa gactataccc cgtatacctt caccaaagat cagattgaag
    36481 aagagttgct gtttatgcca gagggcggca tgccggacat gcaggtgctg acctgggacg
    36541 gtcaactgct ggcgcttgag ctgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac
    36601 caggcatcaa aggggcatct gccagcgccc gtaacaaacc ggcgacattg agcactggtc
    36661 tggtgattca ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc catatcgaag
    36721 aacgccgtta tatggggcgt gctgactaac ttcagccgca tgcagaaaag ggatagctca
    36781 ggctgtccct tttttaattt attataccaa tcttctattt tgcgcttcct gaacacctta
    36841 cgccctcaat tttcactcgt tgattgatcg ccctcacact tcatcgcatt aacaatccag
    36901 accaatttca attgctgtca tataacttta cactgtcatt gttaatttat cgttactaag
    36961 acgtgactcc tatgaagaca attgcctccg ttacgctccc gcatcatgta cacgctccac
    37021 gctacgatcg ccagcagttg caatcacgta tcgttcattt tggctttggc gcctttcacc
    37081 gcgctcatca ggcgttactg accgatcgtg tgctgaatgc ccagggcggc gactggggga
    37141 tctgtgaaat cagcttgttc agcggtgatc aactgatgag ccagctccgc gcacagaacc
    37201 atttatatac cgtgctggaa aaaggtgcgg acggcaatca ggtgataatt gtcggtgccg
    37261 ttcacgaatg ccttaatgca aaactggatt ccttagcggc aattattgag aaattttgcg
    37321 agccacaggt ggcaattgtt tccctgacga ttaccgaaaa aggctattgt attgacccgg
    37381 ccaccggtgc actcgacacc agtaatccgc ggattattca cgatctacaa acccctgaag
    37441 aacctcactc cgcaccgggt attctcgtcg aagcactgaa acgccgccgt gagcgcggcc
    37501 ttacaccgtt taccgtgctc tcctgcgaca atattcccga caatggccat gtggtgaaaa
    37561 acgcggtgct gggaatggca gaaaaacgtt cgccagaact cgccgggtgg ataaaagaac
    37621 acgtcagttt tccgggaacc atggtcgacc gcattgttcc ggctgcaacc gacgaatcac
    37681 tggcagaaat cagccagcat ctgggggtaa atgatccctg cgcgattagc tgcgaaccgt
    37741 ttatccagtg ggtggtggaa gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag
    37801 gtgtacaaat ggtgaatgat gtcctgccat gggaagagat gaaactgcgt atgcttaatg
    37861 gcagccactc ttttctcgct tatctgggtt acctttcagg attcgcccat atcagtgatt
    37921 gcatgcagga tcgcgcattt cgccatgccg ccagaacatt aatgctgaat gagcaagcgc
    37981 cgacactaca aattaaagat gtcgatttaa cacaatatgc ggacaagtta attgcacgtt
    38041 ttgctaatcc ggcgctgaaa cataagacct ggcaaatcgc aatggatggt agccagaaat
    38101 taccgcaacg tatgctggca ggtattcgca tacatctggg gtgcgaaacg gactggtcgt
    38161 tgctggcatt aggcgttgct ggctggatgc gttacgtcag cggcgttgat gatgccggaa
    38221 atgccattga tgttcgcgat ccgcttagcg ataaaattcg cgaacttgtt gcgggcagca
    38281 gcagtgaaca acgcgtaacc gccctgcttt ccctgcgtga agttttcggt gatgatctgc
    38341 cagataaccc gcattttgtg caggccatcg aacaagcctg gcaacaaatc gtacaattcg
    38401 gcgcacatca ggcgctatta aacaccctca aaatttaacg atttctgcgg ttaaagcgga
    38461 tgaagctcac cttcgtccgc tctccccttc tcttttctgc cttttttagc caggattaac
    38521 gctcagttaa cttaccagag tattgcggag ccgttgtgac caggaccaac ctcatcaccg
    38581 gttttctcgg cagcgggaaa accacgtcga ttcttcatct gttagcccat aaagatccca
    38641 acgaaaaatg ggcggtactg gttaatgaat ttggggaagt cggaattgat ggtgctttgc
    38701 tcgccgatag cggcgcattg ctgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg
    38761 gtttacccat gcaggtaggg ttgaatacct tactgcgtca gggaaaacca gaccgcttgt
    38821 tgatagagcc gaccgggctg ggccatccga aacagatcct cgatctgtta accgcaccag
    38881 tctatgaacc gtggatagat ctgcgcgcca ccttgtgcat tctcgatcca cgcctgctgc
    38941 tggacgaaaa aagcgccagc aatgaaaact tccgtgacca gctggctgcc gcagacatca
    39001 ttgtcgccaa taaatccgac cgtgcgacgc ccgaaagtga gcaagcgcta cagcgttggt
    39061 ggcagcaaaa tggtggcgat cgacaattaa ttcacagtga gcatgggaaa gttgacggtc
    39121 atcttctgga tttgccgcgt cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc
    39181 atcagcatgt cgtgaaaaaa gggttagcag cgttaagcct gccagagcat caacgctggc
    39241 gtcgcagtct gaacagcggg caaggatatc aggcctgcgg ctggatattc gacgctgata
    39301 cggtattcga caccattggc attctggaat gggcgcgact tgcaccggtg gaacgcgtca
    39361 aaggcgtgct gcgtattccc gaagggctgg tacgaatcaa ccgtcagggc gatgacctgc
    39421 acattgaaac gcaaaacgtt gcgccaccgg acagccgtat tgagctgatt tccagcagcg
    39481 aagctgactg gaatgctttg cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt
    39541 aaggttgcct gcgtttttca gtaagataat tagagaaaat atgattaaaa atttgccgca
    39601 aatagtgttg ttgaatattg tcggcctcgc gctgtttctt tcctggtata ttcccgttaa
    39661 tcatggattc tggttgccaa ttgatgcgga tattttttat ttctttaatc agaaactggt
    39721 cgaaagtaag gcctttttgt ggctggttgc attgaccaac aatcgcgctt tcgacggttg
    39781 ttctctgctg gcgatgggaa tgttgatgct gagtttctgg ctgaaagaaa acgcccctgg
    39841 cagacgacgt atcgtgatta tgggtctggt catgctatta actgcagtgg tattaaacca
    39901 gctgggtcag gcattaattc ctgtaaaacg ggccagccca acattgactt ttaccgatat
    39961 taaccgcgtc agcgaactgc tctctgttcc cacgaaagat gcctcacgag atagctttcc
    40021 cggcgatcac ggcatgatgc tgcttatttt ttcggcattc atgtggcgtt atttcggcaa
    40081 agttgcaggc cttatcgccc ttattatttt tgtggttttt gcatttccca gagtaatgat
    40141 tggcgcacac tggtttactg acatcattgt cggttcgatg accgtgatat tgatcggttt
    40201 gccctgggtg ttgctgacgc cattaagtga tcgattaatc accttttttg acaaatcact
    40261 accgggaaaa aacaaacatt tccaaaacaa ataactcaca gtaattaaca tcatcaggct
    40321 tatttttata gtgaggataa tcctgatgat gcgcaccgtg ctttcatcca tcgcgcgcaa
    40381 aaatcattct ctaagtaaat gaatggattg catgcgtttc actcaatcgt actttaattg
    40441 accaatcccg cttattaact ttctgtatca ctttttctta taaaaaatca tgtaaaaccg
    40501 ctcgccaaga ccgcaccaat cgggtaatct cgaactcgtt ttgcctcggc ggtagattat
    40561 cctcacagca tataattttg tgcgttagtc cacagatttg gccttaagga attgtttcaa
    40621 catgcccagg taattagtct cgtgtcgctt ggcatttttt tataacgata tttgtcgtta
    40681 aggacttcaa gggaaaacaa acaacatggt caaatctcaa ccgattttga gatatatctt
    40741 gcgcgggatt cccgcgattg cagtagcggt tctgctttct gcatgtagtg caaataacac
    40801 cgcaaagaat atgcatcctg agacacgtgc agtgggtagt gaaacatcat cactgcaagc
    40861 ttctcaggat gaatttgaaa acctggttcg taatgtcgac gtaaaatcgc gaattatgga
    40921 tcagtatgct gactggaaag gcgtacgtta tcgtctgggc ggcagcacta aaaaaggtat
    40981 cgattgttct ggtttcgtac agcgtacatt ccgtgagcaa tttggcttag aacttccgcg
    41041 ttcgacttac gaacagcagg aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg
    41101 tgatttagtt ctgttccgtg ccggttcaac gggacgccat gtcggtattt atatcggcaa
    41161 caatcagttt gtccatgctt ccaccagcag tggtgttatt atttccagca tgaatgaacc
    41221 gtactggaag aagcgttaca acgaagcacg ccgggttctc agccgcagct aataaaccgt
    41281 ttggatgcaa tcccttggct atcctgacga gttaactgaa agcactgctt aggcagtgct
    41341 tttttgtttt cattcatcag agaaaatgat gtttccgcgt cttgatccag gctatagtcc
    41401 ggtcattgtt atcttttaaa tgttgtcgta atttcaggaa attaacggaa tcatgttcat
    41461 acgcgctccc aattctggac gtaagctcct gcttacctgc attgttgcag gcgtgatgat
    41521 tgcgatactg gtgagttgcc ttcaattttt agtggcctgg cataagcacg aagtcaaata
    41581 cgacacactg attatcgacg tacaaaagta tctcgatacc tattttgccg acctgaaatc
    41641 cactactgac cggcttcagc cgctgacctt agatacctgc cagcaggcta accccgaact
    41701 gaccgctcgc gcggcgttta gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa
    41761 aacattctgt tcatctgcga ctggtgagat ggacattcca ctcaatgaat tgattccggc
    41821 gctcgacatt aataaaaacg tcgatatggc gatcttaccc ggcacgccga tggtgccgaa
    41881 caaacccgca atcgtcatct ggtatcgcaa ccctttgctg aaaaatagcg gtgtgtttgc
    41941 cgctctgaat ctcaacctga cgccttctct cttttatagt tcacggcagg aagattacga
    42001 tggcgtcgcc ctcattattg gcaatactgc gctatctacc ttttcttcac gtttgatgaa
    42061 cgttaatgaa ttaaccgaca tgccggtccg tgaaactaaa attgcgggca ttcctctgac
    42121 cgttcggctt tatgcggatg actggacatg gaacgatgtg tggtacgcat ttttactggg
    42181 cggcatgagt ggaactgtcg ttggcctgct ctgctattac ctgatgagcg tacgtatgcg
    42241 ccccggcaga gaaatcatga ccgccatcaa gcgcgaacaa ttttacgtgg cgtatcaacc
    42301 ggtggtggat acacaagctt tgcgagtaac gggcctggaa gtactgctac gctggcggca
    42361 tcctgtcgcg ggagaaattc ccccggatgc cttcattaac tttgccgaat cgcaaaagat
    42421 gattgtgccg ctgactcagc acctgtttga gttaattgcc cgcgatgccg cagaattaga
    42481 aaaagtgctg ccggtaggcg tcaaatttgg cattaacatt gcgccggacc atctgcacag
    42541 cgaaagcttt aaagcagata tccagaaact gctcacttcc ctacccgcac accatttcca
    42601 gattgtgctg gaaattaccg agcgcgatat gctgaaagag caagaagcca cacaactctt
    42661 cgcctggctg cactcggtcg gcgtagaaat tgctattgat gacttcggca ccgggcacag
    42721 cgcgcttatc tatcttgagc gttttacgct cgattatctg aaaattgacc gtggatttat
    42781 caacgccatc ggtacggaaa cgatcacttc acccgtactt gacgcggtgc tgacgctggc
    42841 gaaacgcctc aatatgctga cggttgctga aggggtcgaa acgccggaac aggcgcgatg
    42901 gctaagcgaa cgcggcgtta atttcatgca aggctactgg attagtcgcc cgttaccgct
    42961 ggacgatttt gttcgctggc taaagaaacc gtatacgccg cagtggtaag gtgtgcttac
    43021 gtcccttatt attcatagtg aaagcatgcc ggattgcggc taatgatgag taaaaggaaa
    43081 tccgttgcag atgattgtgc gcatactgct gctgtttatc gctctgttca cctttggtgc
    43141 gcaggcgcag gctatcaagg aaagctatgc ctttgccgtg ctgggcgaac cccggtacgc
    43201 gtttaatttc aaccattttg attatgtgaa ccccgccgcg ccaaaaggtg ggcagataac
    43261 gttgtcagcc ctcggtacct tcgataattt caaccgctat gcactgcgcg gcaacccggg
    43321 cgcacgcacc gagcagctgt acgacacgct atttacgact tccgatgacg aaccaggcag
    43381 ttattacccg ctgattgctg aaagcgcacg ctatgctgac gattattcct gggtggaggt
    43441 cgctattaat ccgcgcgccc gttttcatga tggttcgccc attactgccc gcgatgtaga
    43501 gtttactttt caaaaattta tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg
    43561 caccaccgtc aaagccattg cgccgttaac cgtgcgcatt gagttagcta aacccggcaa
    43621 agaagatatg ttgagtctgt tttcgctgcc ggtatttcca gaaaagtact ggaaggatca
    43681 caaacttagc gatccgctcg ccacgcctcc gcttgccagt ggtccgtacc gaattacgtc
    43741 ctggaaaatg gggcaaaata ttgtctattc ccgcgtgaaa gattactggg cagcaaactt
    43801 accggtaaac cgtggacgct ggaatttcga caccattcgc tacgattatt acctcgatga
    43861 taatgtcgcc tttgaagcgt ttaaagcagg tgcctttgat ttgcgtatgg aaaacgacgc
    43921 taaaaactgg gccacgcgtt ataccggtaa aaatttcgat aaaaaataca tcatcaaaga
    43981 tgagcaaaag aacgaatcag cccaggatac gcgctggctg gcgtttaata tccaacgtcc
    44041 ggtattcagc gatcgccggg tccgggaagc aatcaccctc gcctttgact ttgaatggat
    44101 gaacaaggcg ttgttttaca atgcctggag tcgcacgaac agttattttc agaataccga
    44161 atacgcggcc agaaattacc ccgaagccgc ggagctggtg cttctggcac caatgaaaaa
    44221 agatctaccg tcagaagtct tcacacaaat ctaccagccg ccggtatcta aaggcgatgg
    44281 ctacgatcgt gacaacctgt taaaagccga caaacttctc aacgaagcgg gctgggtgct
    44341 gaagggtcag caacgcgtta atgccacaac gggtcagcca ctcagctttg aattattgct
    44401 tcccgcaagc agcaatagtc agtgggtatt gccgttccag cacagcctgc aacggctggg
    44461 tatcaacatg gacattcgca aggtggataa ctcgcaaatc accaaccgca tgcgcagtcg
    44521 cgactatgac atgatgccgc gcgtatggcg ggcgatgccg tggcccagtt ccgatttaca
    44581 gatttcctgg tcatcggaat atatcaattc cacttataat gcccccggcg tgcaaagtcc
    44641 ggttatcgac tcgctgatca atcaaattat tgccgcgcag ggaaataaag aaaaattact
    44701 gccgttaggg cgagcactgg atcgcgtatt aacgtggaat tattacatgc tgccaatgtg
    44761 gtacatggcg gaagaccgtc tcgcctggtg ggataaattc tcccaacccg ctgtacgccc
    44821 tgtttacagc ctgggtatcg atacctggtg gtatgacgtt aataaagcgg ccaaactgcc
    44881 gtcagccaga caacagggag agtagatggg cgcttatctg attcgccgtc tgttgctggt
    44941 gatcccaacg ttatgggcga ttatcactat caactttttc atcgtgcaaa ttgcgcctgg
    45001 cggtccggtc gaccaggcca tcgccgccat tgagtttggt aatgccggag tattacccgg
    45061 cgcaggcggt gaaggtattc gcgccagcca tgcgcaaacg ggcgtcggca atatcagcga
    45121 cagtaattac cgtggcggac gcggattaga tccagaagtg atcgccgaga tcactcatcg
    45181 ctacggtttc gataagccga tccacgaacg ttacttcaaa atgctctggg actacatccg
    45241 ctttgatttt ggtgatagcc tttttcgcag cgcctcggtg ctgacgctga ttaaagacag
    45301 tctgccggtt tccatcaccc tcggattgtg gagcacgctg attatctatc tggtgtcgat
    45361 tccgttaggc attcgcaaag ctgtttataa tgggagccgc tttgacgtct ggagtagcgc
    45421 atttatcatc atcggctacg ccattccggc ctttttgttt gccatcctgc tgattgtctt
    45481 cttcgcgggc ggcagctatt ttgacctgtt ccctctacgc ggcctggttt ccgctaactt
    45541 tgattcgctg ccgtggtatc agcaaatcac cgattatctg tggcatatca cgctaccggt
    45601 gctggcgaca gtgattggtg gctttgcggc gctgaccatg ctgaccaaaa actcattcct
    45661 tgatgaagtg cgcaagcaat acgtggtgac cgcgcgtgcg aaaggggtaa gtgagaaaaa
    45721 cattctctgg aaacatgtgt tccgcaacgc catgctgctg gtgattgccg gttttccggc
    45781 gacgtttatc agcatgtttt ttaccggctc gctgctgatt gaggtgatgt tttcactcaa
    45841 tggtcttggc ttactgggct acgaagcgac cgtctcgcgc gattatcctg taatgtttgg
    45901 taccttgtat attttcaccc tgattggcct gctgctgaat attgtcagtg atatcagcta
    45961 tacgctggtc gatccgcgta tagattttga gggacgttaa tgtcgcgact cagccccgtc
    46021 aatcaggccc gttgggcgcg ttttcgccat aaccgtcgcg gatactggtc gttatggatt
    46081 ttcctcgtct tgtttggttt gagtttgtgt tctgaactta tcgctaacga taaaccgttg
    46141 ctggtgcgtt atgacggcag ttggtatttc ccgttgttga aaaactacag cgaaagcgat
    46201 tttggcggcc cgctggcaag tcaggctgat tatcaggacc cgtggctgaa acaacggctg
    46261 gaaaataacg gctgggtact gtgggcaccg attcgctttg gtgctaccag tatcaacttt
    46321 gctaccgata agcccttccc ttctccaccc tcccggcaaa actggctggg aacggatgcc
    46381 aacggcggcg atgtgctggc acgcattctc tatggcacgc ggatctcggt tctgtttggc
    46441 ctgatgctga ctctctgttc cagcgtgatt ggcgtgctgg cgggggcgct acaaggctat
    46501 tacggcggta aagtcgatct ctggggacaa cgctttattg aagtatggtc ggggatgccg
    46561 acgctgtttt tgattatttt actttccagc gtcgtacagc ctaacttctg gtggctgctg
    46621 gcaattactg tcttgtttgg ctggatgagt ctggtcggcg tggtgcgggc ggagttttta
    46681 cgtactcgta atttcgacta cattcgtgcg gcacaggcgc ttggcgtcag cgatcgcagt
    46741 atcatcctgc gtcatatgtt gcctaatgcc atggtcgcga ccctcacctt tttaccgttt
    46801 attttatgta gttcgataac caccctgacc tcgctcgatt tcctcggctt cggtctaccg
    46861 ctcggttcac cgtcactcgg tgaactgctg ttacaaggga aaaataacct tcaggccccg
    46921 tggcttggga tcaccgcctt cttgtcggtg gcgatattat tgtctttgct gatctttatt
    46981 ggtgaagccg tccgcgacgc atttgatcct aataaggcgg tgtagcatga cgcaaactct
    47041 gttagcgatt gaaaatttgt cggtgggttt tcgccatcag caaaccgtac gtacagtagt
    47101 caatgatgtt tcactacaga ttgaggctgg tgaaacgctg gcgctggtgg gtgagtcagg
    47161 ttcaggcaaa agcgttaccg cgctgtcaat tttacgcctg ctcccttccc cgccggttga
    47221 atatctctcc ggcgatattc gttttcatgg cgaatcgctg cttcatgtca gcgatcaaac
    47281 gttgcgcggt gtacgcggta ataagatcgc catgattttt caggaaccga tggtgtcgtt
    47341 aaatccattg cataccctgg aaaaacagct ttatgaagtg ctttcactcc accgcgggat
    47401 gcgtcgggaa gcggctcgtg gcgaaattct taactgcctt gatcgcgttg gtatccgcca
    47461 ggcggcaaaa cggctgacag attatccgca tcagctctcc ggcggcgaac gccagcgggt
    47521 gatgattgcg atggcgctgt taacgcgacc ggaattatta attgccgatg aaccgaccac
    47581 cgcactggac gtctctgtcc aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga
    47641 gctgaatatg ggcatgctgt ttattactca taacctcagc attgtcagaa aactggccca
    47701 ccgcgtggcg gtaatgcaaa acggtcgctg tgtcgagcaa aattacgccg ctacgctatt
    47761 tgcatcaccc actcatcctt acacacaaaa gctactcaac agtgaaccgt caggcgatcc
    47821 agtgccgttg ccagaacctg cctcaacgtt gctggatgtt gaacagcttc aggttgcctt
    47881 ccccattcgc aaagggattt tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat
    47941 cagttttacg ctacgagcgg gtgaaacact gggtttagtg ggcgagtccg gttccgggaa
    48001 aagtacgacg ggactggcgc tgctgcgact gattaattct cagggcagca tcatctttga
    48061 cggtcagcca ctgcaaaatt taaatcgccg ccagctgtta cctattcgtc atcgcattca
    48121 ggtggtattt caggatccaa actcctcgct caacccacga ctcaacgttt tgcagattat
    48181 tgaggaaggc ttacgggttc accagccgac gctttctgcc gcacaacgcg aacaacaagt
    48241 gatagccgtg atgcatgaag tgggattaga tcctgaaaca cgccaccgtt atccggcgga
    48301 gttctctggt ggtcagcgac aacgtattgc gattgccagg gcattaattc ttaagccctc
    48361 gctgatcata cttgatgaac cgacatcatc actcgacaaa acggtacagg cgcaaatatt
    48421 gacgctattg aaatcattgc aacaaaagca tcaactggcc tatttgttta tcagccacga
    48481 tttgcacgtt gtccgcgcgt tatgtcatca ggttatcgta ctgcgacaag gggaagtagt
    48541 ggaacaagga ccgtgcgcgc gcgtgtttgc cacaccgcag caggagtata cgcgtcagct
    48601 actggcgttg agctgacgct taaaaaggat tgtagtttga aaagggttcg gcaattgcca
    48661 caccaaaatt tttcaatcgg catgttgcag caaactcgtc ctggcgattt acaaacaggc
    48721 acggctcacc ttcacactcc agcaccgaac atggaacttc gatgtcgctt aacgcctggc
    48781 ttagtttata catcaccgac catgcattat caccatccgg gctaagcaat ttaagcgcca
    48841 ccaggctgtt gtcaccgcac ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga
    48901 aaccagcaga ccagcgatag ttctgcggta gacgatggac gattgagagt tgtaatgaag
    48961 tcacttattt tccccggaag tacatttact tcacaatttg tttacatcaa ttttaacaca
    49021 tcatcaacaa tccgtcttta tacagatcgt aaagattgat gcctcgcctg agtggcctct
    49081 atggctctga tttaagtaat atctgcgggc gtggtcacgt ttgcggggct atatgtaccc
    49141 gtttctgcga tctaactcaa cctttttaac tacaatg
//
LOCUS       DAPHDX010000015        48810 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPHDX010000015 DAPHDX010000000
VERSION     DAPHDX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.015"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(101..1303)
                     /gene="ubiI"
                     /locus_tag="RH058_000843"
                     /gene_synonym="visC"
     CDS             complement(101..1303)
                     /gene="ubiI"
                     /locus_tag="RH058_000843"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV3136469.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1326..2504)
                     /gene="ubiH"
                     /locus_tag="RH058_000844"
                     /gene_synonym="visB"
     CDS             complement(1326..2504)
                     /gene="ubiH"
                     /locus_tag="RH058_000844"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV3136470.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2501..3826)
                     /gene="pepP"
                     /locus_tag="RH058_000845"
     CDS             complement(2501..3826)
                     /gene="pepP"
                     /locus_tag="RH058_000845"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV3136471.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3852..4430)
                     /gene="ygfB"
                     /locus_tag="RH058_000846"
     CDS             complement(3852..4430)
                     /gene="ygfB"
                     /locus_tag="RH058_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV3136472.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4598..4927
                     /gene="zapA"
                     /locus_tag="RH058_000847"
     CDS             4598..4927
                     /gene="zapA"
                     /locus_tag="RH058_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV3136473.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4969..5152
                     /gene="ssrS"
                     /locus_tag="RH058_000848"
     ncRNA           4969..5152
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RH058_000848"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5173..5775
                     /locus_tag="RH058_000849"
     CDS             5173..5775
                     /locus_tag="RH058_000849"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV3136474.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5876..5935)
                     /locus_tag="RH058_000850"
     CDS             complement(5876..5935)
                     /locus_tag="RH058_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3136475.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6268..6327)
                     /gene="ibsC"
                     /locus_tag="RH058_000851"
     CDS             complement(6268..6327)
                     /gene="ibsC"
                     /locus_tag="RH058_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV3136476.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6556..7788)
                     /gene="serA"
                     /locus_tag="RH058_000852"
     CDS             complement(6556..7788)
                     /gene="serA"
                     /locus_tag="RH058_000852"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV3136477.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8044..8703)
                     /gene="rpiA"
                     /locus_tag="RH058_000853"
     CDS             complement(8044..8703)
                     /gene="rpiA"
                     /locus_tag="RH058_000853"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV3136478.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8759..8944)
                     /locus_tag="RH058_000854"
     CDS             complement(8759..8944)
                     /locus_tag="RH058_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136479.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            9086..9979
                     /gene="argP"
                     /locus_tag="RH058_000855"
     CDS             9086..9979
                     /gene="argP"
                     /locus_tag="RH058_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV3136480.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10183..12327
                     /gene="scpA"
                     /locus_tag="RH058_000856"
     CDS             10183..12327
                     /gene="scpA"
                     /locus_tag="RH058_000856"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV3136481.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            12320..13315
                     /gene="meaB"
                     /locus_tag="RH058_000857"
     CDS             12320..13315
                     /gene="meaB"
                     /locus_tag="RH058_000857"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV3136482.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13326..14111
                     /gene="scpB"
                     /locus_tag="RH058_000858"
     CDS             13326..14111
                     /gene="scpB"
                     /locus_tag="RH058_000858"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV3136483.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14135..15613
                     /gene="scpC"
                     /locus_tag="RH058_000859"
     CDS             14135..15613
                     /gene="scpC"
                     /locus_tag="RH058_000859"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV3136484.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNNYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15610..16506)
                     /locus_tag="RH058_000860"
     CDS             complement(15610..16506)
                     /locus_tag="RH058_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136485.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16673..17413)
                     /locus_tag="RH058_000861"
     CDS             complement(16673..17413)
                     /locus_tag="RH058_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV3136486.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(17506..18141)
                     /gene="argO"
                     /locus_tag="RH058_000862"
     CDS             complement(17506..18141)
                     /gene="argO"
                     /locus_tag="RH058_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV3136487.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(18280..19140)
                     /gene="mscS"
                     /locus_tag="RH058_000863"
     CDS             complement(18280..19140)
                     /gene="mscS"
                     /locus_tag="RH058_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV3136488.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(19498..20577)
                     /gene="fbaA"
                     /locus_tag="RH058_000864"
     CDS             complement(19498..20577)
                     /gene="fbaA"
                     /locus_tag="RH058_000864"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV3136489.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(20792..21955)
                     /gene="pgk"
                     /locus_tag="RH058_000865"
     CDS             complement(20792..21955)
                     /gene="pgk"
                     /locus_tag="RH058_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV3136490.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(22005..23024)
                     /gene="epd"
                     /locus_tag="RH058_000866"
     CDS             complement(22005..23024)
                     /gene="epd"
                     /locus_tag="RH058_000866"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV3136491.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            23396..23827
                     /locus_tag="RH058_000867"
     CDS             23396..23827
                     /locus_tag="RH058_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV3136492.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            23850..24428
                     /locus_tag="RH058_000868"
     CDS             23850..24428
                     /locus_tag="RH058_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV3136493.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            24429..25136
                     /locus_tag="RH058_000869"
     CDS             24429..25136
                     /locus_tag="RH058_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV3136494.1"
                     /translation="MHPFTSLTLWALVACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            25124..25801
                     /locus_tag="RH058_000870"
     CDS             25124..25801
                     /locus_tag="RH058_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDV3136495.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            25795..26472
                     /locus_tag="RH058_000871"
     CDS             25795..26472
                     /locus_tag="RH058_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3136496.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(26444..27157)
                     /locus_tag="RH058_000872"
     CDS             complement(26444..27157)
                     /locus_tag="RH058_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV3136497.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(27154..27663)
                     /gene="fumE"
                     /locus_tag="RH058_000873"
     CDS             complement(27154..27663)
                     /gene="fumE"
                     /locus_tag="RH058_000873"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV3136498.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(27685..28650)
                     /gene="yggF"
                     /locus_tag="RH058_000874"
     CDS             complement(27685..28650)
                     /gene="yggF"
                     /locus_tag="RH058_000874"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV3136499.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCREMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(28647..29924)
                     /locus_tag="RH058_000875"
     CDS             complement(28647..29924)
                     /locus_tag="RH058_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV3136500.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLLKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(29939..31327)
                     /gene="cmtA"
                     /locus_tag="RH058_000876"
     CDS             complement(29939..31327)
                     /gene="cmtA"
                     /locus_tag="RH058_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV3136501.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(31355..31798)
                     /gene="cmtB"
                     /locus_tag="RH058_000877"
     CDS             complement(31355..31798)
                     /gene="cmtB"
                     /locus_tag="RH058_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV3136502.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(32112..34103)
                     /gene="tkt"
                     /locus_tag="RH058_000878"
     CDS             complement(32112..34103)
                     /gene="tkt"
                     /locus_tag="RH058_000878"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV3136503.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            34381..35139
                     /gene="loiP"
                     /locus_tag="RH058_000879"
                     /gene_synonym="yggG"
     CDS             34381..35139
                     /gene="loiP"
                     /locus_tag="RH058_000879"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV3136504.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(35195..35938)
                     /locus_tag="RH058_000880"
     CDS             complement(35195..35938)
                     /locus_tag="RH058_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV3136505.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(35925..37034)
                     /gene="dgaE"
                     /locus_tag="RH058_000881"
     CDS             complement(35925..37034)
                     /gene="dgaE"
                     /locus_tag="RH058_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HDV3136506.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(37038..37898)
                     /locus_tag="RH058_000882"
     CDS             complement(37038..37898)
                     /locus_tag="RH058_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDV3136507.1"
                     /translation="MMSDDVMQHELVERARESGTLTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEVINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(37895..38644)
                     /locus_tag="RH058_000883"
     CDS             complement(37895..38644)
                     /locus_tag="RH058_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV3136508.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(38670..39155)
                     /locus_tag="RH058_000884"
     CDS             complement(38670..39155)
                     /locus_tag="RH058_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDV3136509.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(39166..39594)
                     /locus_tag="RH058_000885"
     CDS             complement(39166..39594)
                     /locus_tag="RH058_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3136510.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(39713..42511)
                     /gene="dagR"
                     /locus_tag="RH058_000886"
     CDS             complement(39713..42511)
                     /gene="dagR"
                     /locus_tag="RH058_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HDV3136511.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(42770..43690)
                     /gene="speB"
                     /locus_tag="RH058_000887"
     CDS             complement(42770..43690)
                     /gene="speB"
                     /locus_tag="RH058_000887"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV3136512.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(43826..44557)
                     /locus_tag="RH058_000888"
     CDS             complement(43826..44557)
                     /locus_tag="RH058_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136513.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(44703..46679)
                     /gene="speA"
                     /locus_tag="RH058_000889"
     CDS             complement(44703..46679)
                     /gene="speA"
                     /locus_tag="RH058_000889"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV3136514.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(46688..46819)
                     /gene="yqgB"
                     /locus_tag="RH058_000890"
     CDS             complement(46688..46819)
                     /gene="yqgB"
                     /locus_tag="RH058_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV3136515.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            46955..47170
                     /locus_tag="RH058_000891"
     CDS             46955..47170
                     /locus_tag="RH058_000891"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136516.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            47474..48628
                     /gene="metK"
                     /locus_tag="RH058_000892"
     CDS             47474..48628
                     /gene="metK"
                     /locus_tag="RH058_000892"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV3136517.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT        11899 a        13279 c        12137 g        11495 t
ORIGIN      
        1 gaggtgaaat tagtttattt caaatgagga aaatctcccg gcgaaaaaac cgggagatga
       61 aagtgtgatg ggtatcaaat aaacagcaga agagaaattt ttaacgcagc cattcaggca
      121 aatcgtttaa tcccattgcc tggcggataa gttgtggttt aacgccagga agcgtgtcgg
      181 ccagtttcaa accaatatca cgcagcagtt ttttcgccgg attggtaccg gaaaacagat
      241 cgcggaatcc ctgcatacca gccagcatca gcgccgcact gtgcttgcgg ctacgctcat
      301 accggcgcag gtaaatgtac tgcccgatgt ctttcccctg acgatgcaac cgtttcagtt
      361 cagcaatcag ctctgcagca tccataaagc cgagatttac tccctgcccc gccagcgggt
      421 gaatggtatg cgcggcgtcg cccaccagca ccagacggtg cgcggcaaac tggcgcgcat
      481 aacgccccgt cagtgggaac acctgacgcg cgctctcaac cttgcataag cccaggcgat
      541 tatcaaaagc gatatttaac gcgcgattaa attcatcttc acttgcctgc tgcatccgct
      601 gcgcttcctc tggcgacagt gaccagacaa ttgagcaaag atgcggatcg ctaagcggta
      661 aaaaggccag aatgccttcg ccatggaaaa cctgccgcgc caccgcatca tgcggttctt
      721 ccgtgcgaat ggtcgctacc agcgcgtgat gctgataatc ccagaaagtc agcggaatat
      781 ctgctttgtt gcgcaaccag gaattagcac cgtccgcgcc aatcaccaga cgcgccgtta
      841 acatgctgcc atctttcagc gtcaggaaag tttcattttc tccccaggcg acctgctgta
      901 attctgcggg ggccaacaga gtgatatctg acgactgctg cgctttgttc cacagcgcgt
      961 agtgaatcac tgagttttca acgatatgcc caagatggct atagcccatg ctttgatcgt
     1021 caaacgaaat gtgaccaaaa ctgtctttgt cccacacttc cataccgtga taacaactgg
     1081 ccctacgaga gagaatgtcc tgccagacgc caagacgggt gagtaatttt tcgctggcgg
     1141 cattgatagc cgaaacgcgc agttgtggtg gtgcatccgc cgccagaggt tccggtacgc
     1201 gctgctccag tacggcaacg cgtaagccgc tcccctgtaa gccgcaggca accgccagcc
     1261 ccaccatgcc gccaccaaca atggctacat caacactttg cattgtttat tccttaaaac
     1321 cgccttcaac gcgccaccca accgagggtg cgctgcgcca gcacatcgcg tgccggggtg
     1381 aataattcca tcgtcatcag tccgatgttg cgcccgacaa ccagcggtgc ccaacggttg
     1441 gcaaaaagat gtaccaggct gtccgtgacg ccgatggttg cttcgcgatc actctgtcga
     1501 cgctgctgat agcggcacaa tatgccgtaa tcaccgatgt cttctccgcg ctcatgcgcc
     1561 tgagtcaggg tttccgcaag gctcatgaca tctcgcatac cgaggttaaa cccttgcccg
     1621 gcaatcgggt gcagagtttg cgccgcattg cccaccagca cggtacgatg ggtaatagct
     1681 ctggcggcgc gggttaacgc cagcggataa gcactgcgtt taccggcgtg ggtaattttt
     1741 ccaagtcgcc agccaaaggc cgactggagt tcacggcaaa acttctcgtc gctccacgac
     1801 agcacctctt cgcgccgttc cagtggatga caccagacca gcgaacagcg tccgtcagac
     1861 atcggcaaca tcgccagcgg gccatgttgc gtgaagcgtt caaaagcgcg gccttcgtgc
     1921 gcaacggaag tcgcaacgtt ggcaatcacg gccagttgtt cgtaaggctc ctgctgccag
     1981 tcaacgccgc acgcggtggc taacgctgaa tgggtgccat cagctgctac cagcacgcgg
     2041 cccgtcagcg tctcgccact ctccagcgtc acttcaacgt gactctgagt acgggcaacg
     2101 ttagccacgc gatcagggca atgcagcgtt acgccaggtg ctttacgcag caatgcaaac
     2161 agccgttgcc cgacattgtg caattcgaca acctgtccca gcgccgccag ttggtaatct
     2221 tctgcggcga gggtgacaaa tccagcgtga ccacgatcgc tgacatgcac ggtagtgatg
     2281 gcggttgcgt aatccgccag agattgccag acgccgatgc gcgccagttg ctgacaggtc
     2341 cccgcagcca gcgcaatagc tcgtccatca aagcccggat gagcatgtga ctctggcgcg
     2401 gtcgcttcaa tcaaatgtac cggcagcgcc ccgtgactta accgggaaat agccagcgcc
     2461 agcgtcgcgc ccgccatgcc gccaccgacg atgattacgc tcattgcttt ctcgcagctg
     2521 ccatcaacgc ttcgatttct tccggctttt tcaccacgct ggcggtgagg ttttcgttgc
     2581 cggtttcggt aatcacaatg tcgtcttcaa tacgaatgcc gataccgcga tattgttctg
     2641 gcacatccgc atccggcgcg atatacagac caggctctac ggtcagtacc atccccggtt
     2701 ccagaatgcg cgagcgatcc tgaccataaa cgccaacgtc atggacatct agccctaacc
     2761 agtggctaag gccatgcata aagaaaggac gatgggcgtt ctgagcgatc agttcatcaa
     2821 catcaccttt cagaatgccg agttttacca ggccgctaac catgatgcgc accacttcac
     2881 cagtgacttc cagaatggaa gttcccggac gatacaggcg caagctggtt tcgagagact
     2941 ccagcacaat gtcgtagatt tcacgctggg cctgagtgaa tttgccgttg accgggaagg
     3001 tgcgggtaat atcgccagcg tatcctttgt attcgcaacc cgcgtcaatc aacaccaggt
     3061 cgccgtcgcg catttcacac tcgttttcgg tgtagtgcag aatgcagccg ttttcaccgc
     3121 tgccgacaat ggtgttatag gacggatagc gcgcaccgtg gcggttaaat tcgtggtgaa
     3181 tttcgccttc cagatggtac tcgaacattc ccggacggca tttttccatc gcccgggtat
     3241 gtgccatggc ggtgatttct cccgcgcggc ggagtacggc aatctcttct ggcgatttga
     3301 acaggcgcat ttcatgaaca acaggacgcc agtcgatcat cgttgccggt gcggtgagat
     3361 tttgccgcga acctttacgc agtttttcca gcgcactgtt cacgatttca tcagcatatg
     3421 catattcgcc ctgggcatgg taaaccacat ccaggccgtt aagtagttga taaagttgct
     3481 gattgatttc gctgaatgcc agtgcgcggt caacgcccag tttctctggc gcggcatcct
     3541 ggcctaagcg acggccaaac cagatctccg ccgtcaggtc gcgaacgcgg ttaaacagaa
     3601 cgctgtggtt atgagtgtca tcgcttttaa tcagcaccag caccgcttcc ggttcgttaa
     3661 agccggtgaa gtaccagaag tcactgttct gacgataggg gtattcgctg tcggcgctac
     3721 gtgttacttc tggtgcagca aaaatcagcg cggcgctgcc gggttgcatt tgctccacca
     3781 gggcctgacg gcgacgctga aactcttgcc gggatatctc actcataaca ctctccttac
     3841 gttttttgtt tttagtgtag agtcggtttt tgtacttctg gcgcggtcgg ttgcggatga
     3901 gtaaaggtgt cgtggcataa cagcgcggca acacgaacgt attcgatgat ctcttcaagc
     3961 gacatttcaa gctcttcctg atcttcgtct tcgtcgtaac ccagttgcgc aatgttacgc
     4021 agatcgtcga tagcttcacc ggtttcgccg gtcactttat ccagcttcgg ttgcgtaacg
     4081 ccaagaccaa gcaggaagtg attgacccaa cctgccaatg catcagcccg atcgaaaacg
     4141 ctgacatcat cgccatcagg cagataaagc tgaaaaagga agccgtcatc ctgcagggca
     4201 tcgctggtgg cagagtgcat tttacgcagt gcctgtgcca gctcatgacc gaaagccatg
     4261 ccttcgttcg tcaggtcgtg aagtagcggt agccatgagc tgtcatcgtt accgccacat
     4321 atcatcccac tgattaaacc atgcatctca gcaggggtca gacccgtccc ttgttggttc
     4381 agatactggt tcatttcgtt gtaaccaggc atttcgttct gtatagacat aagcattcgt
     4441 cgtcaaaggg aggaatattc atgatatgct accactttgg gccctggtgg accagaaaag
     4501 ggcttgtctc ttctcatcag ggtagctata gtgtcgcccc ttcgcagacc atgggtctaa
     4561 agacgaaggc agcgcagtca atcagcagga aggtggcatg tctgcacaac ccgtcgatat
     4621 ccaaattttt ggccgttcac tgcgtgtgaa ctgcccgcct gaccaaaggg atgcgttgaa
     4681 tcaggcagcg gacgatctga accaacggtt gcaagatctg aaagaacgca ctagagtcac
     4741 aaatactgaa cagttggtct tcattgccgc attgaatatc agctatgagt tagcgcaaga
     4801 aaaagcgaag actcgtgact acgcggcaag tatggaacag cgtattcgga tgctgcagca
     4861 gaccatagaa caagcgttac ttgaacaagg tcgcatcacc gaaaaaacta accaaaactt
     4921 tgaatgacac ttttcggttt actgtggtag agtaaccgtg aagacaaaat ttctctgaga
     4981 tgttcgcaag cgggccagtc ccctgagccg atatttcata ccacaagaat gtggcgctcc
     5041 gcggttggtg agcatgctcg gtccgtccga gaagccttaa aactgcgacg acacattcac
     5101 cttgaaccaa gggttcaagg gttacagcct gcggcggcat ctcggagatt cccttcttat
     5161 ctggcaccag ccatgacgca actaccagaa ctcccactga cattatcccg acaagaaatc
     5221 cgcaaaatga ttcggcaacg tcgtcgtgcg ttaacgccgg aacaacagca ggaaatgggt
     5281 caacaagccg ctacccggat gatgacttat cccccggtgg tgatggcgca tacggtcgct
     5341 gtattcctct cttttgatgg tgaactcgac acccagccgc taatagaaca actctggcgc
     5401 gccggtaagc gcgtatatct tccagttttg catcccttta gtgccggtaa tttgctgttc
     5461 ctgaattacc atccgcaaag cgaactggtg atgaacaggt tgaagatcca tgagccaaaa
     5521 ctggatgtgc gtgacgtgct acccctttcc cgattagacg tgctgatcac accgctggtc
     5581 gcctttgatg agtacggtca gcgcctggga atgggcggtg gtttttatga tcggacctta
     5641 caaaactggc agcactataa aacgcaaccg gtgggttatg cgcatgattg tcagttggtg
     5701 gaaaaactcc ccgttgaaga gtgggatatc cctcttcctg cggtggttac accgtcgaaa
     5761 gtctgggagt ggtaaggtcg atacacctgc atcgccctga ttgacatcgt tgattcttta
     5821 acctaattta gtgagtaagg gtaagggagg attgctcctc ccctgtgact gactgttaat
     5881 aagctgggaa acttatgagt aacagtacaa tcagtatgat ggcaagtcgc atcataaccc
     5941 ttctccttca agccctcgct tcggtgaggg ctttaccgtt acagccccat gctgcccagc
     6001 tatcgtaagc tctctttaaa taaccacaac gcattgatct acctttaaat cttttcttcg
     6061 agcgtgctcg aaaaacccca tgcaaaacag agataatttt tgggaaaaag gatctattac
     6121 ggcagtaaac gtgggcgatg aagtggctat caaaaagttc ggttcagggc gatgcacccc
     6181 gcatcgccct gattgacatc gttgattctt tgacctaatt tagtgagtaa gggtaaggga
     6241 ggatgactcc tcccctgaga ctgactgtta ataagctccg aaacttatca gtaacagtac
     6301 aatcagtatg atgacaagtc gcatcataac ccttctcctt taagccctcg cttcggtgag
     6361 ggctttaccg ttacagcccc atgctgtccc gccatcgtaa atccccatta aataaacaca
     6421 acgcattgat ctgactttga tttattttct ggagcagact cgcaaagtag aatgcgcaac
     6481 gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg cccgttaatt
     6541 ttcagagaag gggaattagt acagcagacg ggcgcgaatg gtacccggaa tagctttcat
     6601 tgcctgcagc gctttttcgg caacgtcttc gtcagcttca atatcaataa ctacataacc
     6661 catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct cggcgaagat
     6721 tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca gacgacgacc
     6781 accatgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg agccattgtc
     6841 agaatacttg atcaatttac ccgcaacttc caggccgata ttctcctgcg cttcctgagt
     6901 cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagcg gagaggtaaa
     6961 tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc ccgccagatg
     7021 tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc gcgaagcatt
     7081 aatcagcagc gagccgggct tcattagtga aatttctttc gcgcccatca tatttttggt
     7141 ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca ggtcagaaag
     7201 atgctgtacc tgagtggcgt tgcccagcgg cagtttattt tcaatatcat aaaagtaaac
     7261 atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac cgtagccgat
     7321 gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt tccacacgcc
     7381 acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca gttcgccaat
     7441 caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg ggatcccgcg
     7501 ctttgccgcc gcatccagat caacctggtt tgttccgata cagaaacagc caatagcgac
     7561 cagtttttct gcggcgttga tcacgtcttc agtcagatgg gtacgggatc gcaggccgat
     7621 gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc ctttgtgaaa
     7681 ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt gcacgccttc
     7741 taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt acccaatcct
     7801 gtcttttgaa atgttgtgtg cggatttgcg tccgcctttc aacatatcaa aaaataatat
     7861 tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa ggtaattctt
     7921 ggatgaatat gctggtttag aagatttatt tagagcaatc gacaatagcc aggtaaaaac
     7981 gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg ttcccccgtc
     8041 agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa cgtccgcgcc
     8101 acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg cgttttccat
     8161 cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc cattatcggt
     8221 caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctgac gcgccactgc
     8281 actacgtgcc atcgggataa cttctactgg cagcgggaat ttacccagaa tatcaacctg
     8341 cttggaagcg tctgcaatac agataaattt ttctgcaacc gaagcaatga ttttttcacg
     8401 ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt catctgcgcc
     8461 atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa tgccgaggct
     8521 tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct ggcctttcat
     8581 tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac caacaatggt
     8641 gccgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt catcctgcgt
     8701 catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa aaagcgattc
     8761 agaccccgtt ggcaagccgc gtggttaact catccataaa atatcgcgca atggcgggca
     8821 tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc cacggtgcga
     8881 acagaggcaa taacgccacg ccattcaaca caaccagggc gcgaactcgc tgagcgaaat
     8941 gcattagcgc aaatccctga tcttttaagc tgcgcgttta accacgaata caaatgttac
     9001 ccgccgacgg cttcggtata tgcaacctga cacaaaattg tgtcatagtg caggaaaaag
     9061 catttaccag gagcagacaa cagcaatgaa acgcccggac tacagaacat tacaggcact
     9121 ggatgcggtg atacgtgaac gaggatttga gcgcgcggca caaaagctgt gcattacaca
     9181 atcagccgtc tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc cgctgttggt
     9241 gcgtaccgta ccgccgcgcc cgacggaaca agggcaaaaa ctgctggcac tgctgcgcca
     9301 ggtggagttg ctggaagaag agtggctggg cgatgaacaa accggttcga ctccgctgct
     9361 gctttcactg gcggtcaacg ccgacagtct ggcgacgtgg ttgcttcctg cactggctcc
     9421 tgtgttggct gattcgccta tccgcctcaa cttgcaggta gaagatgaaa cccgcactca
     9481 ggaacgtctg cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc aggcgctgcc
     9541 gagctgtctt gtcgataaac ttggtgcgct cgactatctg ttcgtcagct caaaaccctt
     9601 tgccgaaaaa tatttcccta acggcgtaac gcgttcggca ttactgaaag cgccagtggt
     9661 cgcgtttgac catcttgacg atatgcacca ggcctttttg cagcaaaact tcgatctgcc
     9721 tccaggcagc gtgccctgcc atatcgttaa ttcttcagaa gcgttcgtac aacttgctcg
     9781 ccagggcacc acctgctgta tgatcccgca cctgcaaatc gagaaagaac tggccagcgg
     9841 tgaactgatt gacttaacgc cggggctatt tcaacgccgg atgctctact ggcaccgctt
     9901 tgctcctgaa agccgcatga tgcgtaaagt cactgatgcg ttgctcgatt atggtcacaa
     9961 agtccttcgt caggattaat ccatcaaata atgcctgata gcacatatca ggcgttatcc
    10021 tcacttcttt ttgtattcct tgaatcacat cacaaaatag acaaatctca ggcggcaaaa
    10081 aacgacgtct gaatgcattt tttttgctgg cgacaaaccc acgtaaaaag ctcaccgtaa
    10141 gcgcaaatac cctcattttg attgcgtttt acggagcaaa taatgtctaa cgagcaggag
    10201 tggcaacagc ttgccaacaa ggaattgagc cgtcgggaga aaactgtcga ctcgctggtt
    10261 cagcaaaccg cggaagggat cgccatcaag ccgctgtata ccgaagccga tctcgataat
    10321 ctggaggtga caggtaccct tcctggtttg ccgccctacg ttcgtggccc gcgtgccact
    10381 atgtataccg cccaaccgtg gaccatccgt cagtatgctg gtttttcaac agcaaaagag
    10441 tccaacgctt tttatcgccg taacctggcc gccgggcaaa aaggtctttc cgttgcgttt
    10501 gaccttgcca cccaccgtgg ctacgactcc gataacccac gcgtggcggg tgacgtcggc
    10561 aaagcgggcg tcgctatcga caccgtggaa gatatgaaag tcctgttcga ccagatcccg
    10621 ctggataaaa tgtcggtttc gatgaccatg aatggcgcag tgctaccagt actggcgttt
    10681 tatatcgtcg ccgcagaaga gcaaggtgtt acacctgata aactgaccgg caccattcag
    10741 aacgatatcc tcaaagagta cctctgccgc aacacctata tttacccgcc aaaaccgtca
    10801 atgcgcatta tcgccgacat catcgcctgg tgttccggca acatgccgcg atttaatacc
    10861 atcagtatca gcggttacca catgggtgaa gcgggtgcca actgcgtgca gcaggtagca
    10921 tttacgctcg ctgatgggat tgagtacatc aaagcagcaa tctctgccgg actgaaaatt
    10981 gatgacttcg ctcctcgcct gtcgttcttc ttcggtatcg gcatggatct gtttatgaac
    11041 gtcgccatgt tgcgtgcggc acgttattta tggagcgaag cggtcagtgg atttggcgca
    11101 caggatccga aatcactggc gctgcgtacc cactgccaga cctcaggctg gagcctgact
    11161 gaacaggatc cgtataacaa cgttatccgc accaccattg aagcgctggc tgcgacgctg
    11221 ggcggtactc agtcactgca taccaacgcc tttgacgaag cgcttggttt gcctaccgat
    11281 ttctcagcac gcattgcccg caacacccag atcatcatcc aggaagaatc agaactctgc
    11341 cgcaccgtcg atccactggc cggatcctat tacattgagt cgctgaccga tcaaatcgtc
    11401 aaacaagcca gagctattat ccaacagatc gacgaagccg gtggcatggc gaaagcgatc
    11461 gaagcaggtc tgccaaaacg aatgatcgaa gaggcctcag cgcgcgaaca gtcgctgatc
    11521 gaccagggca agcgtgtcat cgttggtgtc aacaagtaca aactggatca cgaagacgaa
    11581 accgatgtac ttgagatcga caacgtgatg gtgcgtaacg agcaaattgc ttcgctggaa
    11641 cgcattcgcg ccacccgtga tgatgccgcc gtaaccgccg cgttgaacgc cctgactcac
    11701 gccgcacagc ataacgaaaa cctgctggct gccgctgtta atgccgctcg cgttcgcgcc
    11761 accctgggtg aaatttccga tgcgctggaa gtcgctttcg accgttatct ggtgccaagc
    11821 cagtgtgtta ccggcgtgat tgcgcaaagc tatcatcagt ctgagaaatc ggcctccgag
    11881 ttcgatgcca ttgttgcgca aacggagcag ttccttgccg acaatggtcg tcgcccgcgc
    11941 attctgatcg ctaagatggg ccaggatgga cacgatcgcg gcgcgaaagt gatcgccagc
    12001 gcctattccg atctcggttt cgacgtagat ttaagcccga tgttctctac acctgaagag
    12061 atcgcccgcc tggccgtaga aaacgacgtt cacgtagtgg gcgcatcctc actggctgcc
    12121 ggtcataaaa cgctgatccc ggaactggtc gaagcgctga aaaaatgggg acgcgaagat
    12181 atctgcgtag tcgccggtgg cgtcattcca ccgcaggatt acgccttcct gcaagagcgc
    12241 ggcgtggcgg cgatttatgg tccaggtaca cctatgctcg acagcgtgcg cggcgtactg
    12301 aatctgataa gccagcatca tgattaatga agccacgctg gcagaaagta ttcgccgctt
    12361 acgtcagggt gagcgtgcca cactcgccca ggccatgacg ctggtggaaa gccgtcaccc
    12421 gcgtcatcag gcactaagta cgcagctgct tgatgccatt atgccgtact gcggtaacgc
    12481 cctgcgactg ggcgttaccg gcacacccgg cgcggggaaa agtacctttc ttgaagcctt
    12541 tggcatgttg ttgattcgtg agggattaaa ggtcgcggtt attgcggtcg atcccagcag
    12601 cccggtcact ggcggtagca ttctcgggga taaaacccgc atgaatgacc tggcgcgtgc
    12661 cgaagcggcg tttattcgcc cggtaccatc ctccggtcat ctgggcggtg ccagtcagcg
    12721 agcgcgggaa ttaatgctgt tatgcgaagc ggcgggttat gacgtagtga ttgtcgaaac
    12781 ggttggcgtc gggcagtcgg aaacggaagt cgcccgcatg gtggactgtt ttatctcgtt
    12841 gcaaattgcc ggtggcggcg atgatctgca gggcattaaa aaagggctga tggaagtggc
    12901 tgatctgctc gttatcaaca aagacgatgg cgataaccat accaatgtcg ccattgcccg
    12961 gcatatgtac gagagtgccc tgcatattct gcgacgtaaa tacgacgaat ggcagccacg
    13021 ggttctgact tgtagcgcac tggaaaaacg tggaatcgat gagatctggc acgccatcat
    13081 cgacttcaaa accgcgctaa ctgccagtgg tcgtttacaa caagtgcggc aacaacaatc
    13141 ggtggaatgg ctgcgtaagc agaccgaaga agaagtactg aatcacctgt tcgcgaatga
    13201 agatttcgat cgctattacc gccagacgct tttagcggtc aaaaacaata cgctctcacc
    13261 gcgcaccggc ctgcggcagc tcagtgaatt tatccagacg caatattttg attaaaggaa
    13321 tttttatgtc ttatcagtat gttaacgttg tcactatcaa caaagtggcg gtcattgagt
    13381 ttaactatgg ccgcaaactt aatgccttaa gtaaagtctt tattgatgat cttatgcagg
    13441 cattaagcga tctcaaccgg ccggaaattc gctgtatcat tttgcgcgcg ccgagtggat
    13501 ccaaagtctt ctccgcaggc cacgatattc acgaactgcc gtctggcggt cgcgatccgc
    13561 tctcctatga tgatccattg cgtcaaatca cccgcatgat ccaaaaattc ccgaaaccga
    13621 tcatttcgat ggtggaaggt agtgtttggg gtggcgcatt tgaaatgatc atgagttccg
    13681 atctgattat cgccgccagt acctcaacct tctcaatgac gcctgtaaac ctcggcgtcc
    13741 cgtataacct ggtcggcatt cacaacctga cccgcgacgc gggcttccac attgtcaaag
    13801 aactgatttt taccgcttcg ccaatcaccg cccagcgcgc gttggctgtc ggcatcctca
    13861 accatgttgt ggaagtggaa gaactggaag atttcacctt acaaatggcg caccacatct
    13921 ctgagaaagc gccgttagcc attgctgtta tcaaagaaga gctgcgtgta ctgggcgaag
    13981 cgcacaccat gaactccgat gaatttgaac gtattcaggg gatgcgccgc gcggtttatg
    14041 acagcgaaga ttaccaggaa gggatgaacg ctttcctcga aaaacgtaaa cctaatttcg
    14101 ttggtcatta atccctgcga acgaaggagt aaaaatggaa actcagtgga caaggatgac
    14161 cgccaatgaa gcggcagaaa ttatccagca taacgacatg gtggcattta gcggctttac
    14221 cccggcgggt tcgccgaaag ccctacccac cgcgattgcc cgcagagcta acgaacagca
    14281 tgaggccaaa aagccgtatc aaattcgcct tctgacgggt gcgtcaatca gcgccgccgc
    14341 tgacgatgta ctttctgacg ccgatgctgt ttcctggcgt gcgccatatc aaacatcgtc
    14401 cggtttacgt aaaaagatca atcagggcgc ggtgagtttc gttgacctgc atttgagcga
    14461 agtggcgcaa atggtcaatt acggtttctt cggcgacatt gatgttgccg tcattgaagc
    14521 atcggcactg gcaccggatg gtcgagtctg gttaaccagc gggatcggta atgcgccgac
    14581 ctggctgctg cgagcgaaga aagtgatcat tgaactcaat aactatcacg atccgcgcgt
    14641 tgcagaactg gcggatattg tgatccctgg cgcgccaccg cggcgcaata gcgtgtcgat
    14701 cttccatgca atggatcgcg tcggtacccg ctatgtgcaa atcgatccga aaaagattgt
    14761 cgccgtcgtg gaaaccaact tgcccgacgc cggtaatatg ctggataagc aaaatcccat
    14821 gtgccagcag attgccgata acgtggtcac gttcttattg caggaaatgg cgcatgggcg
    14881 tattccgccg gaatttctgc cgctgcaaag tggcgtgggc aatatcaata atgcggtaat
    14941 ggcgcgtctg ggggaaaacc cggaaattcc tccgtttatg atgtattcgg aagtgctaca
    15001 ggaatcggtg gtgcatttac tggaaaccgg caaaatcagc ggggccagcg cctccagcct
    15061 gacaatctcg gccgattccc tgcgcaagat ttacgacaat atggattact ttgccagccg
    15121 cattgtgttg cgtccgcagg agatttccaa taacccggaa atcatccgtc gtctgggcgt
    15181 catcgctctg aacgtcggcc tggagtttga tatttacggg catgccaact caacgcacgt
    15241 agccggggtc gatctgatga acggcatcgg cggcagcggt gattttgaac gcaacgcgta
    15301 tctgtcgatc tttatggctc cgtcgattgc taaagaaggc aagatctcaa ccgtcgtgcc
    15361 aatgtgcagc catgttgatc acagcgaaca cagcgtcaaa gtgatcatca ccgaacaagg
    15421 gatcgccgat ctgcgcggtc tttccccgct tcaacgcgcc cgcactatca ttgataattg
    15481 tgcacatcct atgtatcggg attatctgca tcgctatctg gaaaatgcgc ccggcggaca
    15541 tattcaccac gatcttagcc acgtcttcga cttacaccgt aatttaattg caaccggctc
    15601 gatgctgggt taatggtttg tcccgtcacg atcttcagca atatgctgaa gcatcgtggc
    15661 ggtataacga tgatttctga ttgaataaag atattcctgc atatacatcg gcctgtgcgg
    15721 cgcatcaaaa tatttcagcg ttgccggatt caccagtcgc caggcaaaat gcgggatcac
    15781 cgtcagaaaa tgtccgcgct ccacggcgct gattttggcc ataaaactgt agggacgata
    15841 aataatggtt ggattgatcc cacaaggtcg catattcgca tcaatcattg cttcgaagtt
    15901 ggcccggttc tgaaagcgca tttgtagcca gggcagctcc tgaagcagct cttgctcttg
    15961 acgctcttca taacggcgag aaacgagaaa gcctaatcgc agcggtggta gttcactgat
    16021 agtcaggttt tccagttcct gaactcgtgc agatacatgc tgtggggaga taataaaatc
    16081 taactggcgg tcaaaaagat tatcgataac gccgttttcg ctgaactcaa ctggctgggc
    16141 ggtcacgcct tcatacttat ctccgagact aatcaactga tcgaaaataa ttgtcggata
    16201 agtattatct atgccaatca caatttctcg tgaacgacgt cctgagttat ggatttcatt
    16261 atcaatcgca gaaagccgtt gataaaccgg gaacagtttt tgatacagtt cctgtccggc
    16321 tttattgagg ctgatattat tatctttgcg ggtaaatagc gtatagccaa tttgttcttc
    16381 cagcgcggca atgcttttac caaatggcga agccgtcata tggatttttt ctgccgcccg
    16441 ggcaatatta ttggtttgcg ctaataaaat gaaattgcgc atctttttcg aaataaaaat
    16501 gtccatcccc ctcccccgca tccttgtccg cttaaccgtt tcagtcagcc tatccttgat
    16561 gaaaccgcga gcaaagatag gtgattacgt catggtttta cagaaaatta cagaaaaagg
    16621 aggcaatatc gggtaaaggc attagcccga cgaatacgtc gggctacaag tattattgtg
    16681 ctgcaggtgt tttagcgggt tgttgatcca caggttctaa ctggaagacc acatcgacct
    16741 gatcatcaaa ctgaatagcg gcctgctcgt aagtttcctg ggcggacacc ggcgcggcat
    16801 cggctttcat catccgcacc attgggctgg gctgatagtt ggaaacatgg tagcgcacgc
    16861 tatataccgg ccccagttta cgatgaaagc cgttcgccag ttcctgcgcc tgatgaatcg
    16921 cgttatcaat cgctgcctta cgcgctttgt ctttataggc atccggctgc gccacgccca
    16981 gcgacacaga acgaatttcg ttaagacccg ccttcagcgc gccatccagc aaggaattca
    17041 gtttgtctaa ctgacggagc gtgacttcca ccgttctcac agcgcggtag cctttcagga
    17101 tacttttacc atcctgataa tcataatctg gctgggtgcg taagttcgct gagctgatat
    17161 cttttttcgc gatctgattg agttcaagga aggaaatgta ttgtgcgacg cgctcatctg
    17221 cctgtttctt ggcagtagcg gcatccttcg cggccacgtt aacttcaatc gcaagagtgg
    17281 caatgtctgg caccgcatcc acgcttgccg taccggaggt gacaatatgc ggtccatccg
    17341 gcaattcgtt agcctgcgct gccatcccgc taatacccat taatgccgcc agggcgataa
    17401 ctttgaactt cacgttagtc tctccgtgtt aattgacttg cccaaaattc aggacctctg
    17461 gaggcaagct tagcgcctct gttttatttt tccatcagat agcgcctaac tgaacaaggc
    17521 ttgtgcatga gcaataccgt ctctcgccag ctgcaaggct ataaaccaca tgacacatcc
    17581 cactaccaga ttgataatgc gctgtgattt tgccgtgcgc aggcgcggtg ccagccaggc
    17641 tgcgagaata gccagaccaa agaaccacag gaaagaggcg ctaattgtcc cgagcgcaaa
    17701 ccaacgtttt ggttcgacgt caagctgccc gccaaggctg cccagtacaa caaaagtatc
    17761 caggtaaaca tgcggattca gccaggtcac tgccaacatg gtggcgataa ttttccatct
    17821 gccttgcttc aggacttcgg cactggctaa ctcaatatta ctgctcattg cggttttaaa
    17881 agcgccaaaa ccataccaca gcaagaaggc tacgccgccc caggtgacca gcgccagcaa
    17941 ccacggcgac tgcatcaata acgcgctgcc accaaaaatc ccggcgcaaa tcaggaccaa
    18001 atcgctgata gcacaaagta aggcaatcat aatgtggtac tgacgacgaa tgccctgatt
    18061 catcacaaaa gcattttgtg gaccgagcgg taggatcata gctgcgccaa gcgcaagacc
    18121 ttgaaagtaa taagaaaaca cgttggatat tccgaattaa gtatctgatg cagagactat
    18181 atcgcgggat tgttattagc ggaaattgat aatattaatt ggcaatcaga aaaactaata
    18241 agagagaggc cagagagtat tatctggcct cagcgttgat tacgcagctt tgtcttcttt
    18301 cacccgctta aagttcacat ccatttgcgg gtacgggaag ctgataccgg cggcatcaaa
    18361 ttcacgttta atacgctcca gcacatccca gtacacgttt tgcagatcgc cgctgttgct
    18421 ccagacgcgg accacgaaat taatcgacga tgcaccaagt tcgttcaggc gcacagtcat
    18481 ttcgcgatct ttcaaaatgc gatcttcaga ctggataata ttggtcagga tctgcttaac
    18541 ctgatcgata tcggaatcat acgccacgcc aataataaat tcgttacggc gaactggctc
    18601 gcgggagaag ttaataatat ttccggcaat aattttaccg ttcggaataa cgataatttt
    18661 accgtctgca gtacgcatgg tggtggagaa aatctgcaca ctcagcacag taccggctac
    18721 gccgccgagg tcaacatatt ctccggcacg gaacgggcgg aacatgacaa gtaacacgcc
    18781 agcggccagg ttagaaagtg acccctgcaa agccagacca acagctaagc ctgcggcacc
    18841 gagtacagca atgactgacg ctgtttgtac acccacgcgt cccagggcag cgattagcgt
    18901 aaaggcgata ataccgtaac ggactaatgc agaaagaaaa tcagcaacag tggcatcgat
    18961 tttacgggag atcatcaggc gattcaccgc gttggaaatc atccgcgcga taatcaaacc
    19021 aacgatgatg atcgcgagtg ccgccacgat gtttactgca taacttagca gcagcgcctg
    19081 gttagctacc agccagcttc ccgcgccgtt tatgctatcg acaacattca aatcttccat
    19141 tcaatattcc ttttcaactg actcaaaatg gagaaataca ctccgccgcc ttatgacggg
    19201 cagtctgaca agggtaaaca ataaagcata attttgccaa atagatcaca gatttctcat
    19261 ctgaatcacc ttgcttttgg cgaaaaaatc cccgcagagc ggggctcaga cagatatcaa
    19321 tgtgcgcttt gtcatgccgg atgcagcgtg aacgccttat ccggcctaca aaaacctgca
    19381 aattcaatat attgcaggat acgtgtaggc ctgataagcg tagcgcatca ggcaattttg
    19441 cttttggcga aaaaaagacc cgcagagcgg gccttgagat aagcagaaag gaatgactta
    19501 cagaacgtcg atcgcgttca gttcctggaa tgctttttcc agacgagcga tcatcgaagt
    19561 ctgaccggca cgcagccata cgcgcggatc gtagtatttc ttgttcggct gatcttcgcc
    19621 tttcgggtta cccagctgac cctgcagata agcttcgttc gctttgtagt agttcagaac
    19681 gccttcccag gttgcccatt gggtatcggt atcgatgttc attttcacta cgccgtagct
    19741 tacggagtct ttgatttcct gagcagtaga accggaacca ccgtggaata cgaagttcag
    19801 gctgttgtgc ggcaggttgt gtttcttaga aacatattcc tgagaatcac gcaggatggt
    19861 cggagtcaga accacgttac ccggcttgta aacaccgtgt acgttaccga aggacgcagc
    19921 gatggtgaaa cgcgggctga ttttgctcag ctcggtgtat gcgtaatcaa cgtcttccgg
    19981 ctgggtgtac agtgcagaag cgtccatgtg gctgttgtcc acgccgtctt cttcaccacc
    20041 ggtgcaaccc agttcgattt ccagagtcat gccgattttg gacatacgct ccaggtattt
    20101 agagcagatt tcgatgttct cttgcagaga ttcttcagac aggtcgatca tgtgagaaga
    20161 gaacagcggc ttaccggtag ctgcgaagtg tttttcaccc gcgtccagca gaccgtcgat
    20221 ccacggcagc agtttcttcg cgcagtggtc agtgtgcagg ataaccggaa caccataatg
    20281 ttcagccatc tggtgaacgt gatgcgcacc agagatcgcg cccaggatag cagcaccctg
    20341 cggaacgtca gatttcacgc ctttaccagc gataaaggaa gcaccaccgt tggagaactg
    20401 aacgataacc ggcgctttaa ctttagcagc agtttccagt acggcgttga tggagtcagt
    20461 accgacgcag tttactgctg gcagtgcgaa gttgttttct tttgctacct ggaaaacttt
    20521 ctgtacgtca tcaccagtga ttacgccagg ttttacgaaa tcaaaaatct tagacatgtc
    20581 tcttgtcctg tatcgtcggg ccttatactc atcatacttc aagttgcatg tgctgcgtct
    20641 gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact ctcttgtcgc
    20701 cttcctgcaa ctcgaattat ttagagtatg aaaaaatgcg tgctctaaaa gcgcgctgaa
    20761 acaagggcag gtttccctgc cctgtgattt tttacttctt agcgcgctct tcgagcatcg
    20821 ctactgcagg cagtacttta ccttccacga attcgaggaa tgcgccgccg ccagtggaga
    20881 tgtaggagat tttgtcagca atgccgaaca ggtcgattgc tgccagagtg tcgccgccgc
    20941 cagcgatgga gaacgcttcg ctgtctgcga tagcgttagc cacgatttca gtacctttgc
    21001 ggaagttcgg gaattcgaac acgccaaccg gaccgttcca cagaatggtt ttcgcattct
    21061 tcaggatttc agccagttcc tgagcggaag catcaccgat atccaggatc tgctcgtcag
    21121 ctttcacatc gttaacagat ttcagggtag ccggtgcagt ttcagagaac tcggttgcta
    21181 cgcgaacatc agacggaacc gggatgttgc aggtggtcag cagacgttta gcttcgtcaa
    21241 ccaggtcagc ttcgtacagg gatttaccca catcgtggcc ttgtgccgcg ataaaggtgt
    21301 tagcgatacc accaccaaca atcagctggt cagcgatttt agacagggag tccagaacgg
    21361 tcagtttggt agatacttta gaaccaccaa cgatagccac catcgggcga gcaggttctt
    21421 tcagtgcttt acccagcgcg tccagttcag ctgccagcag cgggcctgcg cacgcaacgt
    21481 cagcgaattt accgataccg tgagtagaag cctgcgcgcg gtgagcagta ccgaatgcgt
    21541 ccattacgaa cacgtcacac agtgcagcgt attttttgga cagggtttcg tcgtctttct
    21601 tctcgccttt gttgaagcga acgttttcca gaacaaccag ttcaccttca gcaacgtcaa
    21661 cgccgtcgag gtaatcttta accagacgaa ccgggttaga cagtttgtct ttcaggtagt
    21721 taacaaccgg cagcagagag aattcttcgt tgtactcgcc ttcggtagga cgacccaggt
    21781 gggaagttac catcactttt gcgccttgtt tcagggccag ttcgatggtc ggcagagaag
    21841 cacggatacg cgcgtcgctg gttactttcc cgtcttttac tggtacgttc agatccgcac
    21901 ggataaatac acgtttccca gcaagatcca gatcggtcat cttaattaca gacatggtga
    21961 atcctctcgt tgattctaaa agttttgcag acgctgcttg cgtcttacct gaaagcaaca
    22021 gtagccatag ctaacgtcgt gtcgagcatt cggttagcaa agccccattc gttatcgcac
    22081 cagaccaacg ttttgatcag gtgtgcgcca ctgacccggg tttgggtgcc atcgacaatg
    22141 gcactgtgcg gatcgtggtt aaaatctaca gagaccaacg gcaattccgt atagtcaact
    22201 ataccatgaa atgcaccttg tgctgctttt tgcagcaaca ggttgacttc attggctttt
    22261 acaggtttct tcaccgtcac gcttaaatcg attgccgtca catttatggt tggcacacgt
    22321 accgcaatcg cttcaaagcg atcgttaaat tgcggaaaaa atcgtgtgat accggcggcc
    22381 agtttagtat cgaccggaat gatcgactgg ctggctgccc gggtgcgacg caggtcagga
    22441 tggtatgcat caataacctg ttgatcgtgc atggcggagt gaattgtggt cactgtgccg
    22501 gactcaatac cgtacgcatc atctaacaat ttgatgacgg gaattatgca attcgtggta
    22561 caggaagcgt tagaaacgat gcggtgttcc gcacgaagtt gatcctgatt gacgccgtaa
    22621 acaacggtcg cgtcgagatc gttactgcca ggatgtgaaa agagcacttt tttggccccg
    22681 gcggcaatat gcgcttcgcc atgctcgcgg gagccatata cgccggtgca gtcgaggact
    22741 acatcaacgc caagttcacg ccaggggagc gattgcagtg aacgttcatg caatacgcgg
    22801 atggcgtcat caccaacaaa aagttgatcg cgttcctgtc gtacttccca tgcaaaacgg
    22861 ccatggctgg tgtcatattt caacaaatgc gccatgcccg cagcatccgc cagttcgttg
    22921 attgccacca cggtaatttc cgcccggcgt ccggattcat acaaagcacg aaccacatta
    22981 cgcccgatgc gaccgaagcc atttatcgct acgcgtacgg tcatagatct cctgcaaggt
    23041 tttccctgag caaatttgcc agacagagta atccagcaaa tcgtccgggg aaaccttacc
    23101 tgtcgcaaac tgcgactgat tggttaattg tcgaacattt aatcgactga aacgcttcag
    23161 ctaggataag cgaaacgtgg aataaaagga atgattgtcc agtcaaagaa aacaaatatc
    23221 tgacttacgt cacgttttca gccccgcccc tgtggaattt attccacttt gcagaatcac
    23281 gaataagcgt agcaaaatct tttcgttgtg ccgtcacaaa ccctgactta tatcagaata
    23341 tgactgatgc agcgtcgata ttatccgcaa tacgtccgag tttcggagta ctccaatgat
    23401 gacattgccc tacccatatt cttcctccgc acgcttttac gcactgcgcc tgctccccgg
    23461 gcaggaagtt ttttcccagc tacacgcttt tgcacagcag cagcaacttc atgccgcctg
    23521 gattgcaggc tgtaccggta gcctgactga cgtcgccttg cgctatgccg ggcaagaagg
    23581 caccacgctc ctgaacggta cattcgaagt gatctcacta aacggcacgc tggaacagag
    23641 tggtgagcat cttcatctct gcgtttccga tccacacggc acgatgcttg gcggtcatat
    23701 gatgcccggc tgtaccgtgc ggaccacgct tgaactagtg atcggtagcc ttgaggaact
    23761 ggcgtttagc cgccaacctt gcgcgctttc tggttacgac gagttgcata tttcccccgt
    23821 taaataacta ctttcagaga ggttttctta tggcgcgtag ccacttttcc agtcaggcac
    23881 tggtgttgat cgttatatct atcgccatca atatgatcgg cgggcaactg gccagcatgg
    23941 tcaaattacc tatatttctc gactccatcg gcacccttat tagcgcggta ctgcttggtc
    24001 cggtgatcgg gatgctaacc ggcttactga ccaatttact ctggggattg ctgaccgacc
    24061 cgatcgccgc cgcatttgcc cccgtcgcga tggtcatcgg cctggtcgcc ggttgcctgg
    24121 cgcgagcggg ttggtttcgc accctgccaa aagtcgttgt cagcggcgtg attattacgc
    24181 ttgcggtaac ggtcgtcgct gtaccgctgc gtactgcgct gttcggcggt gtcaccggta
    24241 gcggagccga tttgttcgtt gcctggatgc actcgatggg gcaaaacctg gtggagtcgg
    24301 tggcgattac ggtgataggc gcgaacctgg tcgataaaat tcttacagcg gtgattgtct
    24361 ggcttttact gcgccagctg ccgattcgca ccacgcgcca ttttccggca atggctgccg
    24421 tgcgctaaat gcatccattt acctcattaa cactgtgggc attggtggcc tgcaccacgc
    24481 ttatcctgcc cgcgcagacc attttgccga tctatagcgc ggcgaccttt ttctgcctta
    24541 ttgcgctaaa ggccacgcgg cggcgggcaa aatatgtcgt ctggctgatg ttctcgctgg
    24601 gagccgggct gtggctggta cacggtggct ggctaccaga atggctaagc ggcacgccac
    24661 gctcgccaga acggtggtcg catgctatta cgctctggtt gcggattctg gcgattgttt
    24721 caacctcaca gttgtggatg cagtacgtcc ccgtgcaatg ttttattcgc gctctgtttg
    24781 cttctcgcct gccgccaggc gtcgcttatc tgtttgccgg gccgttattg gtcgttgagc
    24841 agttaaaacg gcaattagcg attattcatg aagcgcagcg tgcccgcggc gtgccgctgg
    24901 atgagggctg gtatcagcgt ttacgtgcga tgcccgcgtt aattattccg ctaacacaca
    24961 atgcgcttaa cgatctggct gtccgtggtg cggcgctgga tatgcgggca tttcggatcc
    25021 acaaccgacg aacaacgttg tgggcaccag ccgatagcac cctacaacgc gtagcgcgtt
    25081 acaccatgat cttgctgatg ctgacagaat tcggagcgtg gatatggtta cgttagaaca
    25141 gttccgttat tgcccgacac actcaacgca tcctccgttc tgttatgact ttcactatgt
    25201 caaaccgggg atggtcgcca tctttggtga caacggcagt ggcaaaagta cgctggcaca
    25261 gctgatggcg ggctggtatc cggactttct gccaggagag ataacaggca cggggacgct
    25321 cctcggtacg cccattgggc atctcccgct taatgaacaa tccgccacca tccagctcgt
    25381 ccagcaatcg ccctatttgc agctctccgg ctgtaccttt agcgtggaag aagaagttgc
    25441 ctttgggcca gaaaatctgt gtcttgctga agcagagatt atggcgcgga ttgatgccgc
    25501 cctgactctg accgagtgcc agccgttgcg ccatcgtcac ccggcaacac tctccggtgg
    25561 agagacacag cgcgtggtca tcgcctgcgc catcgcgatg cagccaaagc tcttaattct
    25621 tgatgaagcc tttagccgcc tgactccgca ggccagcgaa atgctgttgc agcgcttaca
    25681 acactgggcg tttgaacgtg gttcgctgat tattcttttt gaacgtcatc gcacaccttt
    25741 cctgaactac tgccagcaag cgtggcaatt gcaaaacgga gcgctccagc cactatgtta
    25801 acgttaaata agatatctta tcgctggcct ggcgcggcaa cggattgtct ttgcgacatt
    25861 tctctgcaac tcaaacaagg tgaatggttg gcgctgaccg gcgacaatgg cgcgggaaaa
    25921 tcgacgctgt tacgagtaat ggcaggtctt cttacgccca cagccggtac ggttatgctg
    25981 caacaacagg cgatgaaaaa cctgaaaaac cgccagcgcg cggcaaaggt tggcgtactg
    26041 tttcaggaag cggaaaacca actttttcac agcaccgtcg ctgacgagat cgcatttggt
    26101 ttgaaactgc aaaaatgccc ggcggatgaa atcactcaac gtacccatgc tgccctgcaa
    26161 tgttgtcagt tagcagacac tgccaactcg cacccgttgg atctgcatag tgcgcagcga
    26221 cggatggtcg ccgtcgcctg cctggaagca ctctctccgc cactattgct gctggacgag
    26281 ccgagtcggg attttgacga aaattggttg agcgtgtttg aaagctggct ggagaagtgc
    26341 cgccagcgag gcacgagcgt cgtagcaatc agtcatgacg ccgcatttac ccggcgtcat
    26401 ttctctcgtg tggtgcgact ggaagatggt ctaatcagga acgttaatcc accagacgat
    26461 attcacccgt agcggtcatc attaaggtca aatttgcagg cagactctct tccagcaccc
    26521 ggcgaacatt cggcccgtcg gtacggtcat aaaaagcttc tgcgtctgcc agagataccc
    26581 ctcccgccag cttgcgccct accagtcgtt cccgcaacgc agtagctggc gcgttgataa
    26641 agatcgagaa atcacaaaat tgcgcaagct ggcaccactt ctcgtcgtcc agcaacaacc
    26701 agtttccttc gacaatgacg agtggtgcgg taacgtgcaa cgcatcttca acaggatcat
    26761 gcttttgtcg atcgtactgc ggccacgtac aatccccttc cacgacctgg cgcagatttt
    26821 ccgccagttt cgcaacgtca aatgtctctg gtgcgccttt gaaggggcgc aattgatgcg
    26881 catccagcca gctattgtaa tggtgaaaac catccatcgg gagcgtctga atagcgggca
    26941 gttccgggtc ttgttgcgcg agatattccc agaaggtggt tagggttgat ttacccgtcc
    27001 ccggcggagc gcaaagaaaa accaccgtac gccgctgcgg atttaccgtc tgtaaggccg
    27061 ccaacatgtg cagcaacggt ttatgcacgc gttctatctc ttcatcatgg tactgcgctt
    27121 gcacgttcag cccattcacc gttaattcaa ttttcacggc gttaattcct ttaaggtggt
    27181 cgttactgcc agcgtcagcg ccgttttgac cattccgcta aaacgcgcct ctgaccagac
    27241 ggcgaaatcg gcgaacttca tcgctttcaa cgcgtcatag agcgtcgcat tgcaggttat
    27301 gctattgacg ttgctgataa aatcccaggt catgtcatcg gcaaatccgg gcgtttcatc
    27361 ctgttcgttc aggtaatgcc agtactggga aaagtgcgtg atatcggcat acagccattt
    27421 atccattttc cccagcgcat aaatcaaacg cagcgcaaca tcaatatcgt caagcgggcc
    27481 gctttgggcg agtaacggct tcactgcata ttccacgatc tcttcatcgt tatcgacaaa
    27541 cagcgacggc agaaactggc gtatcccctg gagcaaaata gaatgcgcag ttttcataaa
    27601 tgaaaataaa ttgtcctgtg catccagttg ctcaagcaca tcatcttctg tcagcgtcgc
    27661 cattggtctt ccgatagatc accatcaatg cagggagtct attatattac acgtttggtc
    27721 cgcgccgccg atcagtaatg tctgcgtccg caccccattc gccgtctgtt ggataccgtt
    27781 caccaactcc ccgcccgtca cacccgtagc gctaaagagg atatcgttcc ccctcaccag
    27841 ttcatcgagc gagtacacac ggttaacgtc aacgcccatt tctctgcaac gcttgcgctc
    27901 ctgctctgca atctgccgat tttccgtgta atccccttta gcctgacaaa agtcaatcag
    27961 ctcggcctgc atatccccac ctaatgcttt aacggcacag gcagaaatca cgccctccgg
    28021 cgcaccgccg atggtgtaca tcacgtcgta aggattatcc tgccagcagg tcagcacgct
    28081 ggcggcgaca tcgccatccg gcagagcgaa aaccttcacg cccagctgtg ttgcttcttc
    28141 aatcgcggca ctcaggcgcg gtttatccag cgtaaccatg cgcagcttgt ccagcggttt
    28201 acctaacgcc cgcgccacgt tacgcaagtt gtccgccaga ggcaaagaca gatcaatcgc
    28261 cccggcagcc agtcgattaa caaccagctt tttcatatac atatcgggcg catgtaacag
    28321 gctatctcga ggggcgaaag ccatgaccgc cagcgcattg ctctgcccca tcgctaccat
    28381 ccgcgtgcct tcaatcgggt caaccgcgat atcgacttct ggcccatcgc ctttgcccac
    28441 ctcttcgcca atccacagca tcggcgcatg gtcaatttct ccttcgccga taaccactcg
    28501 tccacgaaaa gcgacgtcgt ttaatgcctg gcgcatcgcg gtgaccgcca ggccatcaat
    28561 tttatttttg tcgccacatc cggtttgcgg ccaggcagcc aacgctgcct gttccgtaac
    28621 gcgaaataat ggccacgcca gggacatcat tgcgcggcct ccccgatatc aacgccaaat
    28681 tgtgccagca gatatttctc tgcctgttca ttccagatgc cgtgcgtttc ctcaaccagc
    28741 cgcgccaact cttttaacag cggatcggtt ttgccttttt cggcaaaatc ggcaatggca
    28801 gtgagcggca tggtcacgcc gttataaatg agttttttac cgccagggat atccggcaga
    28861 ttgagcacgg tatctggcac cgcatccagc ccaccgatat gcgtgaccat aaaggacggc
    28921 tgcaactgcc ccgtggcgct taacgcaatc gcctctttca tgtcgtccgt tgaaccgccg
    28981 gatgtaccga ctacgtgcgt gctgttgtaa tggacgttgt agaaattaaa cggcactttg
    29041 aagtttttat ccgtcggccc ggcaaagaag ttcagacagc catcttccgc cagtaattcg
    29101 tcagccatct caacgacagc aggcaccgcc gcataaacaa aaatgtcatc gaacccgaca
    29161 tctcctgtca gcgcccgcag cgtctggacc ggatcgctca tccctttggt attcacatac
    29221 accagctcaa tgcctttact ggccgccaga tccaccggca gcagcttctg cacctgcgcc
    29281 aggcgtttgt cgtcgatatc gaccaccacc acccgtgacg gttgtatgcc accgttaatg
    29341 gcgtaatcga tagcgccaat gcccatcgga cccgcacacg ccagcagtgc aatattgccg
    29401 ccaggtttga cgcccatgcg atgctcataa acatattgcg tggtgtgata attggcatga
    29461 taagcaccaa taatgcagca cataggctct gccagcgacg ccgcagcaaa ataagagccg
    29521 tgatacggta atacgcagcc caaattaatg gcgatttcgg gaataatcat ataggtggcg
    29581 ttaccgccaa aatattcgta gctgtagcct gctgaatatc cgcttggtaa ccccatcgcc
    29641 ggttgcaata caaaacgctg gcctttttta tatttgccag tgagattttt acccacttcg
    29701 acaataaccc cggcacattc atgcccggta atgaccggat gattttctaa atcgtcgggt
    29761 acgcgtttat gttcactacc gagtaacgcc gctttccagg tcgataaaca gacgctgtca
    29821 gaaattacac tcactaacag ttcattatcg gtaatttctg gcagttcaaa ttcgcgcaga
    29881 cggacatccc gcttgccata aatagcagca actttggttt tcatttttac ctctgtaatc
    29941 agtgtttatg ttcggcggtt aattgattaa ataaagtgtc gagttttgga tcgccaatat
    30001 agttattaat caatatcagc ggtttatccg tcacacgttt cacgcgccct tccagactgg
    30061 cgtgagtgac gacgatatcg gcatccgcag gcacgttttc tatcgcgtaa tgttttactt
    30121 ctattgccag ccccgctttt tccaggcgtt tacggaaggt ggtcgcgccc atcgcactgg
    30181 agcccatccc cgcatcgcaa acaaaggcga tacgcttaac cctgcttaac gagaatgcac
    30241 cctcttgttt catcgcctta accgcattgg ctgactgagc aaactcatct tcgctctccg
    30301 tttccaccgt tttttccatc ttcagtatca gcgaagtaat agcaaaggac accagggtac
    30361 ctaccgtaac accggcaatt gtcgccagga acgagccttt cggcgtcagt gccaggtaag
    30421 caaagataga ccccggactt gggccagcca ccagaccacc gtccagtaag ttaaacatcc
    30481 aggtaccaga cataccgccc gcaatcatgg caataatagt cagcggcttc atcagcacat
    30541 atgggaaata cagttcgtgg atcccaccga ggaagtgaat aatcatcgcc ccgggcgcag
    30601 aacgtttact catcccttta ccaaacaagg taaacgccag cagcagcccc aggcccggac
    30661 ctgggttaga ggccaccata aagaagatgg atttaccgtt aaccgaagcc tgttgcattc
    30721 ccagcggata atagacgccc tgatcgatcg cattattaag gaaaagcact ttcgccggtt
    30781 cattgatgac tgacagcaga ggcagataac ccgcatgtac cagcgcctcg atacactctt
    30841 tgacgaaagt attggcaatt aacaccgccg ggccgatcac ttcaaaaccc agcagacaaa
    30901 ggagcatccc cgcgatacct aatgagaagt tattgataac catctcaaaa ccggcaggta
    30961 tgcgtttttc cagtgcctta tcgacatatt ttatgaccaa cccaccgagc ggccccatga
    31021 tcattgagcc aaggaacatc gggatctctg cgccaacgat cacaccaata gtacctattc
    31081 cgcccatgac cgccccgcgt ttaccgccga ccagatgacc acctgtagaa ccaatcatca
    31141 cgggcaataa ataggtaatc atcgggccga caattttggc gaaatgttcg ttaggcagcc
    31201 aaccggtggg aataaataac gcagtaataa aaccccaggc aataaaagca ccaatattgg
    31261 ggatgaccat tgcagtcaaa aagcccccaa aagcctggac ctttgcacga gcagacttgt
    31321 tttccataat attatcctgt agaggagaga gtaattatcc gcggctgata atgtccgcta
    31381 attgtttttc tgatgatgct gttaagagtt gttcgagtat ttcttcttcg cacagtaatt
    31441 cacttaacgc ctgaatagcg ccaatgtgcg aatcggcatc ggcagcagag agtccgatga
    31501 gtaatttaat tggttcgtca ttccccggaa aataaacacc ttgttcaagt aatgtcaaag
    31561 acatcccggt tttaagcgcc ccacattccg gtcgcgcatg aggcattgcc acgcctggtg
    31621 cgagaatata ataagggcca ttgttaatgg tggaatcttt aattgcctga atgtaattct
    31681 cgctgatata gtttttatcc agcaatgata ccatcgagaa atcgatagct tcctgccaat
    31741 cttttgctga atgtataact gagattgatg attctggaaa ataatcacta agccgcataa
    31801 attatcctta ttttattttt agggtaccgt ccggcggtaa aaataaaata ctcgtgcggt
    31861 ttaatgaaag caattgagca gggaaaccga tgttttgaaa acagtgaatt attaatttct
    31921 tatataacat tgagttatag atataacaac aaatgtcaat acgcatatcg tgatgcatat
    31981 tacataattg atatttattg catataaata tttgtgatct acaacacgct ttatctattg
    32041 cttgtccgca aacggacata tcaaggtaat aaaaaaggtc gccgaagcga cctttttacc
    32101 cgaaatgcta attacagcag ttcttttgct ttcgcaacaa cgttatcaac agtgaagccg
    32161 aactcttcaa acagcagctc tgccggagca gattcaccga aggtggtcat accgacgata
    32221 gcaccgttca ggccaacata cttgtaccag tagtcagcaa tacccgcttc tacagcaacg
    32281 cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag cagcatcctg cttgtcaaat
    32341 gcgtcggtag acggcatgga caccacgcgc gctttcacgc cttcggcagt cagtttttcg
    32401 taggcagcaa cagccagttc aacttctgaa ccggtagcga tgaaaatcag ttccggctga
    32461 ccggcgcagt ctttcagcac ataaccaccg cgcgcgatgt ttgccagttg ctcttcagtt
    32521 cgttcctgct gcgccaggtt ctgacgggag aggatcagtg cggtcgggcc gtcctgacgc
    32581 tcaacaccgt atttccacgc gaccgcggat tcaacctggt cacacggacg ccatgtagac
    32641 atgttcgggg ttacgcgcag agaagcgacc tgctctaccg gctggtgagt cgggccatct
    32701 tcgcccagac cgatggagtc gtgggtgtaa accatcacct gacgctgttt catcagcgca
    32761 gccatacgta cggcgttacg tgcgtattcc acgaacatca ggaaggtgga ggtgtacggc
    32821 aggaaaccac cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa ctcgcgaaca
    32881 ccgtagtgga tgtagttacc cgcagcatct tcgttgattg ctttagaacc agaccacagg
    32941 gtcaggttag acggtgccag gtcagcagaa ccgccgagga attccggcaa cagcggaccg
    33001 aaagcttcga tagcattctg agacgcttta cggctggcga ttttcgccgg gttagcctgc
    33061 agtttagcaa tgaactcttt tgctttcgcg tcgaagtcag acggcatttc gcctttcata
    33121 cggcgggtaa attcagcggc ttcctgcgga taagctttcg cgtaagcagc gaatttctcg
    33181 ttccatgcgg attctttcgc ctggcctgct tctttcgcat cccactgagc atagatttca
    33241 gacgggattt cgaacggtgc atatttccag cccagctgtt cgcgggtcag ggcaatttca
    33301 gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg ggaaccgaaa
    33361 ccgatgatgg ttttgcacat cagcagggac ggtttgtcag tcactgcgcg cgcttcttct
    33421 actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt cgcgaataac gtgccagccg
    33481 taagcttcga aacgcattgc ggtgtcgtcg gtgaaccagc cttcaacgtg accatcgata
    33541 gaaataccgt tgtcatcgta gaatgcaatc agtttaccca gcttcagcgt acccgccaga
    33601 gagcaaactt cgtgggagat gccttccatc atgcagccgt cgcccatgaa ggcgtaggtg
    33661 tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt tttttctgca
    33721 atcgccatac cgactgcgtt ggcaataccc tgacccagcg gaccggtggt ggtttccaca
    33781 ccagcggtat aacctacttc cgggtggccc ggggttttag agtgcagctg acggaagttt
    33841 ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta gatcagcatg
    33901 gagccgtggc cgttggacag cacgaagcgg tcacggtcag cccaggacgg attctgcggg
    33961 ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt cagccatacc cataggggca
    34021 cccgggtgac cggatttggc tttctgtact gcgtccatgc tcagcgcacg aatagcattg
    34081 gcaagctctt tacgtgagga cattttgact ccagatcgga tgatgaaggg cacgccctta
    34141 acgacttgac gacagcgcgt tttgggctac gccggaaaat ttgccaacaa tttaccgcaa
    34201 gccgcgcgtc atgtacatgg aacatccttt tgccgcttca gaaatctctg gatcatgctc
    34261 gcatgttgcg caatcttctc gcccgtccgc tgcgcttttc cttatactga gactgagcgt
    34321 cgattcacct gcaaacggcg catttttaga ataatcctga ccttgtgcgg aagagaaaac
    34381 atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa cggtactgac tggttgccag
    34441 aatatggact ccaacggact gctctcatca ggagcggaag cttttcaggc ttacagtttg
    34501 agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc aggagatgga cagcaaggcg
    34561 acgattgcgc cagccaatag cgaatacgct aaacgtctga caactattgc caatgcgcta
    34621 ggcaacaata tcaacggtca gccggtgaat tacaaagtgt atatggcgaa ggatgtgaac
    34681 gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg ggctgatgga tatgatgacg
    34741 gataacgaag tcgaagcggt gatcggtcac gagatggggc acgtggcgtt aggccatgtg
    34801 aagaaaggga tgcaggtggc gcttggtaca aatgccgtgc gagtagctgc ggcctctgcg
    34861 ggcgggattg tcggcagttt atctcaatca caacttggtg atctgggcga gaaattagtc
    34921 aattcgcaat tctcccagcg ccaggaagca gaagccgatg attattctta cgatcttctg
    34981 cgccaacgcg gcatcagccc ggcaggtctt gccaccagct ttgaaaaact ggcaaaactg
    35041 gaagaaggtc gccaaagctc aatgtttgac gaccatcctg catccgccga acgcgcccag
    35101 catattcgcg atcgtatgag cgcggatggg gttaagtaag tcagacatac cgatctacaa
    35161 cataattgta ggtcggtaac gcctgaatcc ccgatcagag tagtgatttc actttattta
    35221 gcaaaacggc tacatcatcc ggtcgggtat atcccgtatc agggtctata atcgagctgt
    35281 aaacatgtgg cataatgcgc ggcacccccg cctccagaca agttttcaga atcacactga
    35341 agttatccaa atcaatcccg ccagtcggct caatcagcgt cataccattg cgcgccgccg
    35401 tggtcgccag cgcgtatagc tccggaagcg acctctcgcc gcccatcgga aagaacttcg
    35461 ccgcgtgtgc gcccatatct agcatcattc gcaccgccgt atcgcaggag acgcgggcag
    35521 gcgtaccctg gctgctgcta acgccggtgg aaatcagcac ttcgcccggc gtccccgtcg
    35581 ggctaaccag cgcgttgaca tgggtctgct caccgcccgt agccgcaagc gcgcctgccg
    35641 cgaaaccaca accggtaaag gtctggttaa cgtgtgctgg atgaatctgc gccgcaatca
    35701 tcgcagcttt gtaatactgc gccgggtcac ccgcccccag acctactgaa atggacggta
    35761 tttccgccat ccaacgttta acttcctgga tcccctcatc caccgaggag aactgcgcgg
    35821 aaagcacgcc aatcactgcg tggccttcgg cggcgtcaaa gatttcacgg gcattggcga
    35881 tatctttcgc cagcacatta attgccacgc gattacgata gaaattaatc ttttgcatga
    35941 tctgcaatct ccttcagtcg cgccacgata agtgccattt ccccttccgc caccgtacgc
    36001 ggatcaaggc taaaaacgcc ctggtggagg ttataccgcc gtgcataaat cgcgatatcg
    36061 ccaccacgca gttgcgcttc gacgacgcga gcgtcaactc ccaattcctg cgcatttact
    36121 cgaatacgaa tacgccagat tgcacgcccg gcttcatcct gctcaatatc agccgttaac
    36181 ccgtgaatcg cagaaatcgc ttcaaccact ggctggagct gttccgcggt gatgactgcc
    36241 tgcccctggt gataattctc cagcgcgtaa accagcccaa ccatgttctc cttgccgatt
    36301 ttcatcgccc tggcaatgcc gtgatgctgc gctttgcagg ctgcaatcca gattttttta
    36361 ccagtgataa acccggaggt cggcgcgtta aaggcttttg cgccgctgta gaccaccata
    36421 tccgctccgc tcaccaccca ggcacgtaaa tcctcttcgg cggcggcatc gacaatcagc
    36481 ggcaggttgt acatctgcgc gacatgtgcg aagtcctcga tgttcaacat tcccttctgc
    36541 acgcagtgat gcgacttcac atacagcagc gcggcggtcg cgtcggtaat ggcactctcc
    36601 agctgccaga gagccgccag gttacttgaa cccacctcca caacgcgccc gccacctaag
    36661 cggatcgcgc tgaggatcga tgcaccgtaa tcgacgttat ggccgcgcag catcaccact
    36721 tcattggcca tgccggagct atccggcatg agcgccaccc gggccttgtc gccgcgtgta
    36781 atggcggcgg cgacagcgat ggcgatccct gctgaggcac aggaggtaac gtagctatcc
    36841 tccgccccgg taaagcgaga gaccagctcg ccggttttgt ccaccagctg gtcaatctcc
    36901 acgaaagagg atgccgcgcg cgcggtggcc tgcatcacct ccggcgcgac gctggagacg
    36961 ccgagaatcg tcatcttgcc gcaggcgtta atcacctgtt tcagtcccag ttgttgatag
    37021 atattctgcg tcatggctta cagcactccc agcagcgagc agaccacgct caacgccaca
    37081 atcgacagca gaatcgtggt gtaacgcgga ccctttttca ccaaatagaa gtagatggcg
    37141 aacactgccg ccagcggcag caggccgggc gcgatggagt ccagaatctg ctgtaccacc
    37201 acttcagagc ctttcagtgc gctaattttt aaaggtgagg ttatcttgac aaagctcgcg
    37261 gatagcgcgc ccatcatgat aagccccagt acgttagcgc cgtagataag ctccttgatg
    37321 cgccctccct gcagcagact gataatcgaa tcgcgcccca gcgtatagcc cttgtgcacc
    37381 atgccgtagc tgattgccag cgtaatcgcc gggtagagaa tcagcggcaa aataccgccc
    37441 atcgcgctgc cattggcggc gaacgggata aaaatagcaa tcaacagcgg catcaccgcc
    37501 gcccagatga tggagtcgcc catcccggcc agcggcccca tcaggccggt tttaatcccg
    37561 gtgatagagg catcgttaat cacctcgccg cgggtctttt gctcttccat ggcgatggag
    37621 atcccctgga tcaccgcgcc aaacgtctgc tctgagttga agaagttcag atggcgtttt
    37681 aacgcctcca cctgctcctc cttttgcgga tagagctttt tgataatcgg cgtcatcgag
    37741 gcgcagaaaa tcaggctctg taaacgctca taggagctgg aaacttccgc gcccagccag
    37801 tagataaacc acgctttggt gatatccgct ttggtcagcg tcccggactc ccgcgcgcgc
    37861 tcaaccagtt catgctgcat tacgtcgtcg ctcattatgc cgctccttca tttttcgcca
    37921 ggcctttaat caggaaggcc acgcaggtgc cgaaaatcgc catcgccatg atgtccacct
    37981 tgaggtacag caccgcgaag aatccgccga taaaccacgg cagcaggctc tttttaccaa
    38041 tcaccataat ggtgatggcg aagcccagcg ccgggagaat cccgcccatg atttcaaacg
    38101 agtgggtcag ccagtgcggc atcagcttga ggaaactttc cactacgttc tggccgaagt
    38161 agttggcggc gaacaccact gggaaacgta gtaccagccc cagcagcgcc gggtagagaa
    38221 aggcgcagcg cataatggct gacatattgg ccgtttccgc gtgtttatcg gccatatgca
    38281 cccaggccgc atttagcgtg cgacgcaact ggtcgaggaa tacgccgata acgccaaatg
    38341 gaatcgccag tgcaatcgcc aggttcgggt ccatattggc cttaacggcg ataggaatgg
    38401 cgatacaggc cgccagcgcc gggtctgacg gcacattgcc gcctggcgtt gaggtcacac
    38461 ccagatagac caactgcatc cccgcgccga taatcatcgc cgcaggcata ttgccgagca
    38521 gcaggccgac aaagaccgcc accaccactg gctgtaacag catggcggag aaggtatagc
    38581 caaggcgcaa ccgggcaaac cagtaataca gccccatcag gcttgcaaaa agtaaggtat
    38641 ccataatttg ttatcccatg ttcagagggt cagaattttt tcaggatatc gtccagcgac
    38701 tgcggtttat cttccgggat ggtctggaaa acagccttaa tgccgcgact tttcaaatcg
    38761 ttaagaatgc caacgtcttt ttcatccagc gttatgttct ggaacaccgc tttacggttt
    38821 ggcccgccgc ctaaaccgcc aacctgaata tcagccacgt caaagccctg ctgcaccgct
    38881 tcctgaatcg ccgccagcga cgggaacagc accagcacgt tgccgtcgcc cagctggttc
    38941 tctttccacg cggccgcgaa gctctggttg ctaaagcagt ccactttgat attcggcggc
    39001 gcggccatca ggtagatatt tttcataaac ggatcggcgt ccagctcgtc gctgaccacc
    39061 gcaatacggt tggcctgaga ctgccctacc cacttcgtca ccacctgtcc gtggataaga
    39121 cggctgtcaa tgcgacataa cacgatgttt gccatacgat tttccttact gttgtaattg
    39181 acgaatatgg gagacgacgt ccaggcaact gttgcgccca gcttccacca gatcggccac
    39241 gcaggcggtc agtgggccat gttcgcgctt gtccagcgcc tccagcagca gcgaggcatt
    39301 aagcccggag atcaccgcca ccggataatc ggcgctcagc cgcgccgcca cgttagaggt
    39361 ggtgccgccg ataaaatcgg tcacgataag cgaaccttcc ggcatggttt tcaccacggc
    39421 ttcgacgcgc tggtaaaact cgcccagcgt atcgacgggc atcagcgcga tttccgtcac
    39481 gccttcgatt tcgcccatca ccatgcgcag actgttgcag agctgcagcc cccagccgcc
    39541 gtgagttagc agcagaattt caggtaacgg atgaataggt acagttgtgg tcaaaatagc
    39601 ctcctttgct ccagtgttta catttcatca ggagcaaagg acgtgccaga atttgtgtca
    39661 cgcacagagg aaatgagatt ggatggggcg ttaagccccg taaaccaggg gatcagctgt
    39721 agagcagttc gtagatataa aaatattctg cgtccgataa acggatagcg taagcctctt
    39781 caatcggcaa aaaggaggat ttgatgacgc taaaggcacg agcgtccaga tcaagccggt
    39841 tttctagcgc catctgtaac ggtttgcggt taatcacgat acgctcgacc atgcagcagc
    39901 agtggatcag aaaacgtagt gtcacctggc ggctcggttt cagggagagc gtgttggtta
    39961 agtagttcag aacatcctcg atctctttca gaattcgctg cgggttgagc accgaaatat
    40021 ggttgatgat gctctccatg gtcagcgcgc tgataaagcg catggcgctg cgctccatct
    40081 ccagtcggcg ctcgctgctt gagagatccg gcgtcagcag gctgagcacc agctccggcc
    40141 cttgctcgga aaacagttcc tccagcgaga taaacggtat atccggcagt cccggctgga
    40201 aggtgccgac gatccctgcc agccgttcac tggcattaag cgcctgctga atacgctcca
    40261 ggctgcgcac ctcgttatag tcgagaatca ccatacgcgt gtcctgcgac atcagctcgc
    40321 caaagctctc ctccagcacc tttttgatct tttccgccgt ccccattccg gttatacatg
    40381 agataaccag cactttgcca ccgctctctt gctgcggcgt gcagagccga caagggatat
    40441 ttttactctg cataagcaca gccaattgcg atagatcgcc agtttcgtag cttaaatcca
    40501 gcccaacctc cagcaggctg gtcagagtga tatttggcaa caacagaacg tcaatctgga
    40561 acagcttgct gatggtgctg ccaaaatgga ccagcgatcc gatatccacc atcagtatca
    40621 gccgctgcca gcaccggtcc tgaatcatcc gggtcagggt ttccagggtg tcatgcaccg
    40681 actgctcaaa tggcatatca attgcgtaga acagatcgcg ctccagcacc cggttcacat
    40741 attgcgcctg gctcgttgcg gtggtcgcac cgtgagcgat tagaatcacg ccgcaatccg
    40801 ggctggtgtc gatgcgctgg cggtagtggc gacactcttt caggaacagg cacagccaga
    40861 ccacctccgt cgccgggcac tgaatatgca acaactcatt gattttgcgg cacagaagcg
    40921 tggcattgtc atactcatct ttacagcgat ccagaatcag gctggatgaa tagagttgag
    40981 gtataagccc tcgccgcaca tagccaatca gcgcaagaaa gtgtttacgt aacgggttcg
    41041 ccaggttttc tggcaatgta aatcctaata cctgctccac gtaaccgatc agcagcgtca
    41101 cacgctcctc aatttgatcg ccatagcgcg gtggatgcgc cgcactatgg cggctataaa
    41161 gaccatattc aaaaatcgaa ctgagtttgt ttttcaggat agccagcgtt tctgcgggcg
    41221 gcacgttact gttgcgcaga ttgacatatt cacgagtcag gaaactgtaa aacaggtcgc
    41281 tctcttctat ttccgcgctg gtcgccaacg aattttttaa cgcgggcaac gtacgcgcat
    41341 ccacgttcag acgatcttta ccgccaaaca acgcatcgac cagcagacgc tgctctggcg
    41401 tggtattaaa cgacatctcc gccagtcgtt tatctaactc tagcgtatca ttacgctcgg
    41461 tcattcccga cgcccacgcc tgcgcgcaca agaactgaat atcactttta agctggccga
    41521 tattgccttc caggggttta cttaacaacc aatgcaagag cgttttatca aggcttatgg
    41581 tacgttcaat tttacgactt tcacgctgaa agaagcttac aatcaattcg agctgctcct
    41641 cgaccgaacg ctggcgaata cccggtaagt caatacatac ctggatacgc cgctggaagg
    41701 tacgcaatag tgccgagttg accgactctg tagtggcgca aataaggcgc accgaaattg
    41761 aatatgttgg cccacttgaa ccaagtgggc ggtattcacc tttatcgaga atagaaaaga
    41821 gcttctcttg tccttcgtac gggaggcgat gcacttcatc cagaagtaaa taacctccgt
    41881 cagcttgctc caccaatccc gttttattct cacttgcccc ggtaaatgcc cccttatggt
    41941 gaccaaacag atgtgaggaa agcaactccg gattatgagc atattcggca cagttaaagt
    42001 aaaccaacgg aggtggcgaa cctatggtgt gcttgcaagc gaagcaatgc attaactcag
    42061 caaagaatgt tttgccaaca ccagatgcac cggttaacag cacatgtagt ccgtggggat
    42121 aaagcaccgc tgcgcggcct ttttctaccg catcacggag actacggtcg tatccgataa
    42181 gtccggaaaa cggatcatca tctgtatatt tttctttctt tggcaataaa ctagccactg
    42241 aatgaactat gcattcgcaa tcatccagtt ttcgattaat gagcaattct agtgcgtgac
    42301 gatgtagaaa aaaaacagga cgtccctgac tttttaccac caggccttca ttccataatt
    42361 gattaagatc tttactaacc gagttgcgtg ccagcccaag gttaaaacca atcgcctcag
    42421 cagtaaatgc tttctccttc gctaaatcag acagttccag cccatacgtc agccgctcca
    42481 gctccttgtg aataacatca atccgtctca tttctttacc gcttaaaaaa caatagttgt
    42541 tcagaacata cactatttgt aaacgtaaga atgagaacta tcgggaagaa ttaatgttcc
    42601 ttgtggcaat ctggtttctg aatattcgtg ttttaaatat tacttatggt ctgaaacaac
    42661 caggcaggtg aacagagcta agaaaaagta gcgtagaaat ataaaaacat atatcgcact
    42721 gatgaatgtt tcccggcatg actatgcaca ccgggaaata tttagtaaat tactcgccct
    42781 ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcagtga
    42841 tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg atgttgagat
    42901 ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt cagaccgcca attaccggcg
    42961 taccggttcc cggcgcaaat gcaggatcca ggcagtcgat atcaaaggtc aggtaaaccg
    43021 gcatatcacc cacaatttgt ttcacctggg cgataacgtc atccacgctg cgatcgttca
    43081 cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaattcggta cgaataccaa
    43141 tctgcacgga atgattcgga tcgatcagac cttctttcgg cgcggtatag aacatggtgc
    43201 cgtggtcaaa ttcacaaccg ttcgcgtagg tatcggtgtg ggcgtcaaag tgtaccagcg
    43261 ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac
    43321 caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct
    43381 tctcgctcat ctcacgggca tcgccgaaag catataccag atcaccgcag tccacgacgt
    43441 tcagacgctc acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg
    43501 tcgaaacctg acggatagct gccggaccgt ggcgtccccc cgcacgacca gaagtggcca
    43561 tatcgaacgg cacaccagta atcacccagt cagcatcgct gtcatacggc tggaagttca
    43621 tcggcaggcg taaaaaacca aaggcattgg aaaccagtga gttatcgtat tgatgaccta
    43681 aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt
    43741 aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccttaa
    43801 atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca tagccattgt
    43861 tatctgctgg aaaagcttgt ccagagcacg gtggcagcgc gttttgcgcc gggccttgta
    43921 attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc gccgaattgg
    43981 ctgcgtagga atcaacaacg ttggcgaact gaaaagccgc ttcggtatgt ttatctttgc
    44041 ggaatccttt aatcgctaac gaatctccgg cattcacaat atagccgttg ttattcagcg
    44101 aaccttgctt gccgttcagc acatccaggc catcaaccgt agcaacgatc tcgtagttgg
    44161 tgttacgact gtaattacga acatataatt gataattcat tccgactctg gcgtggacaa
    44221 tatagcgtgg ttcattattg gatgaatcat aaacacgcgt aataggtaga gaattaaaat
    44281 taccatcgcg aatagcatat tccagttcgt taatacgaat gctataaacg cggtcgtagc
    44341 cggaaggaac attagtcgaa taactaataa cgatcatctc cgccggatcg cgccaggctc
    44401 gctcagcaga tacgccctga accgttgagt gcacatcccc accccaggcg gtgccgacat
    44461 tacccgatgc actgctatta tactgcgcat tactggaaca accggccagc agcacgatta
    44521 atgccaccaa cgcgctacgt actttccatt ttttcataac cgttccctct gcatttaatt
    44581 aatgcagcga ttgtagggag attatttaat aagaaaagca gctaaattat ttttaagaat
    44641 atataaaaag aaagcggacc gatttacgta aataaatcga cccgccagca gaataatctt
    44701 aattattcat cttcaagata ggtataaccg tacaaacctg cctcgaactc ttcaaggaac
    44761 tgttgttgca gttcagcatc aagatcggtt ttcttcactt gatcacggaa ctgagttaac
    44821 agcgttttcg gatcgagctg tacatattgc agcatgtccg ccacggtatc gccttcgtca
    44881 gacagttcca cttcgacact accgtcaggg aacacgaaca cgtcaaccgc ttcggtatca
    44941 ccgaacaggt tgtgcatgtt gccgaggatc tcctgatatg cgccgaccat aaagaaaccg
    45001 agcatcggcg gattctctgg atcgtactcc ggcataggca ttgtcgtggc aataccgtca
    45061 ccatcaatat agtggtcgat agcaccgtca gagtcacagg taatatccag cagcacagcg
    45121 cggcgttccg gcacttgatc cagcccttcc agcggcagaa ccgggaacaa ctggtcgatc
    45181 ccccacgcat ccggcatcga ctggaacagc gagaagttga cgtacatttt gtccgccata
    45241 cgttcctgca gctcgtcgat aatcggacga tgagcacggt tttgcggatc cagctgcttt
    45301 tgcacttcat ggcacatgct caaataaagc tgctcagccc atgcgcgttc ttgcaggcta
    45361 aaggtgccgg aagagtagcc gatatgaatg tcgtgcagat ccatctgact gtcgtgtaac
    45421 cattcacgca gagaacggcg agttcccggt tcgtgcatct cctgccaggt ttcccacatg
    45481 ctttgcagcg cacgcggcgc atcttctgca ggcgcggtcg gcaccgtgta ttcgttacgt
    45541 tccacgccga tgatattaga caccagcacg gtgtgatgcg cagtcactgc acgacccgat
    45601 tcagtgatta ccgtcggatg tggcagaccg ttttcttcac atgcatcacc aatcgcccag
    45661 atgatgttgt tggcatattc attgaggccg tagttcaccg aacagtcgga ctgcgaacga
    45721 gtaccttcat aatccacgcc cagaccgccg ccgacgtcga agcactgaat attgacgccc
    45781 agcttgtgca gttccacata gaaacgcgcc gattcacgaa cgcccgtggc gatatcgcga
    45841 atattcgcca tctgcgaacc gaggtggaag tgcagtagtt gcaggctgtc gagacgcccg
    45901 gcttcacgca gggtttcaac cagttgcagt acctgagtcg cagccaggcc gaacttcgat
    45961 ttttccccgc cggaggactg ccatttaccc gagccctgcg aagccagacg tgcacgcacg
    46021 cccagacgag gaacgacatt cagacgttct gcttcatcca gcacaatggc gatttctgac
    46081 atcttctcaa tgaccagata gaccttgtgg cccatcttct cgccaattaa tgccaggcgg
    46141 atatattcgc ggtctttata accgttgcag acgatgacgc tacgggtcat gccagcatgc
    46201 gccagcaccg ccatcaactc tgctttcgag cctgcttcca gccccaacgg ttcgccagaa
    46261 tgaatcaggg actcaatcac gcggcggtgc tggttaactt tgatcggata aacaaggaag
    46321 taatcgccgt tatagccgta ggattcccgc gcacgtttga acgcggcgtt aatggaacgc
    46381 aaacggtgct gcaggatctg tgggaaacag aacagtgcag gcagacgctg accctgtgct
    46441 tcacgagttt tcactaactg cgcgagatcg acgcgagctt ccgggacgtc cgggtccggg
    46501 cacacgctga tgtggcccag ctcgttaacg tcatagtagt tattgcccca ccaggcaata
    46561 ttgtaagtac gcagcatctt gctggcttcc tgggagctca ttgcaacctc ctgcatggag
    46621 cgtagtacac cgtgttcgcc cgctgacgaa ggcaaaccca tagacatgtc gtcagacata
    46681 gcgaacctca aattatttta ttaagtgtaa aacagttaac gactatcgca gcctggaact
    46741 gcgataacaa cccataagca catggatttt ccagcagtga atgctgacgc tccaactgcg
    46801 caaccggttt ctttttcata acattattaa gcacataacc gaacgtaagt gtgacagttc
    46861 ggcgaaacca cgagaaaact cttgttttta caagagcgcc cttgttcagt cctcagtaac
    46921 tgtaaccagc tcttgaatcc tgagaagcgc cgagatgggt ataacatcgg caggtatgca
    46981 aagcagagat gcagagtgcg gggaacgaat cttcaccaga acggtgagac aggttaagca
    47041 gcagacaacg gttcattatt tcgtatcacc tccacggccg cctgttaaga cgaacccaca
    47101 agccaaaact ctgatttcaa cccggctgga agtggcaaca cgaaagaaac gtcgtgtgct
    47161 ttttatttaa gccgcgcgcc gcgttttata ccccacaatg gcagaaaatt gcaaaagata
    47221 aatacgcaga atgccggcat tgtcaggaaa aatttccagc cacgttttta acagaatgag
    47281 acatgattca aaaaaaagtg gaaatcgggt gaagaattga cctaaaatag ccatccagat
    47341 gttaatccat ccataccgat taacactcag actgccagtg tttttaactt gcagagtcgt
    47401 ggtaggatcc gctaccacag aaaatccaca caacagtttg agctaaccaa attctcttta
    47461 ggtgatatta aatatggcaa aacacctttt tacgtccgag tccgtctctg aagggcatcc
    47521 tgacaaaatt gctgaccaaa tctctgatgc cgttttagac gcgatcctcg aacaggatcc
    47581 gaaagcacgc gttgcttgcg aaacctacgt aaaaaccggc atggttttag ttggcggcga
    47641 aatcaccacc agcgcctggg tagacatcga agagatcacc cgtaacaccg ttcgcgaaat
    47701 tggctatgtg cattccgaca tgggctttga cgctaactcc tgtgcggttc tgagcgctat
    47761 cggcaaacag tctcctgaca tcaaccaggg cgttgaccgt gccgatccgc tggaacaggg
    47821 cgcgggtgac cagggtctga tgtttggcta cgcaactaat gaaaccgacg tgctgatgcc
    47881 agcacctatc acctatgcac accgtctggt acagcgtcag gctgaagtgc gtaaaaacgg
    47941 cactctgccg tggctgcgcc cggacgcgaa aagccaggtg actttccagt atgacgacgg
    48001 caaaatcgtt ggtatcgatg ctgtcgtgct ttccactcag cactctgaag agatcgacca
    48061 gaaatcgctg caagaagcgg taatggaaga gatcatcaag ccgattctgc ccgctgaatg
    48121 gctgacttct gccaccaaat tcttcatcaa cccgaccggt cgttttgtta tcggtggccc
    48181 gatgggtgac tgcggtctga ctggtcgtaa aattatcgtt gatacctacg gcggcatggc
    48241 acgtcacggt ggcggagcat tctctggtaa agatccatca aaagtggacc gttccgctgc
    48301 gtacgcagca cgttatgttg caaaaaacat cgttgctgct ggcctggccg atcgttgtga
    48361 aattcaggtt tcctacgcaa tcggcgtggc tgaaccgacc tccatcatgg tagaaacttt
    48421 cggtaccgag aaagtgcctt ctgaacaact gactctgctg gtacgtgagt tcttcgacct
    48481 gcgcccatac ggtctgattc agatgctgga tctgctgcac ccgatctaca aagaaaccgc
    48541 agcatacggt cactttggtc gtgaacattt cccgtgggaa aaaaccgaca aagcgcagct
    48601 gctgcgcgat gctgccggtc tgaagtaatc tttcttcacc tgctttcaaa ggccagcctc
    48661 gcgctggcct ttttctttta cctcaccgcg gtggataggc attcacgccg catccggcaa
    48721 aaagctgccc gcacaataac atcattcttc ctcaacacgt tttatcgcaa attatcatca
    48781 ctactgaaac cgattacacc aataacacca
//
LOCUS       DAPHDX010000016        47953 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPHDX010000016 DAPHDX010000000
VERSION     DAPHDX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.016"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1694)
                     /gene="rsxC"
                     /locus_tag="RH058_000893"
     CDS             complement(<1..1694)
                     /gene="rsxC"
                     /locus_tag="RH058_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV3136518.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
     gene            complement(1687..2265)
                     /gene="rsxB"
                     /locus_tag="RH058_000894"
     CDS             complement(1687..2265)
                     /gene="rsxB"
                     /locus_tag="RH058_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV3136519.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2265..2846)
                     /gene="rsxA"
                     /locus_tag="RH058_000895"
     CDS             complement(2265..2846)
                     /gene="rsxA"
                     /locus_tag="RH058_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV3136520.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2923..3363)
                     /locus_tag="RH058_000896"
     CDS             complement(2923..3363)
                     /locus_tag="RH058_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV3136521.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3449..3664)
                     /gene="ydgT"
                     /locus_tag="RH058_000897"
     CDS             complement(3449..3664)
                     /gene="ydgT"
                     /locus_tag="RH058_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV3136522.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(3937..4062)
                     /gene="blr"
                     /locus_tag="RH058_000898"
     CDS             complement(3937..4062)
                     /gene="blr"
                     /locus_tag="RH058_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV3136523.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            4305..5345
                     /locus_tag="RH058_000899"
     CDS             4305..5345
                     /locus_tag="RH058_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3136524.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGEQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(5380..6381)
                     /gene="add"
                     /locus_tag="RH058_000900"
     CDS             complement(5380..6381)
                     /gene="add"
                     /locus_tag="RH058_000900"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV3136525.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6485..7657)
                     /gene="malY"
                     /locus_tag="RH058_000901"
     CDS             complement(6485..7657)
                     /gene="malY"
                     /locus_tag="RH058_000901"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV3136526.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(7667..9259)
                     /gene="malX"
                     /locus_tag="RH058_000902"
     CDS             complement(7667..9259)
                     /gene="malX"
                     /locus_tag="RH058_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV3136527.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            9434..10462
                     /gene="malI"
                     /locus_tag="RH058_000903"
     CDS             9434..10462
                     /gene="malI"
                     /locus_tag="RH058_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV3136528.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            10574..11341
                     /gene="hdhA"
                     /locus_tag="RH058_000904"
     CDS             10574..11341
                     /gene="hdhA"
                     /locus_tag="RH058_000904"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV3136529.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            11570..12160
                     /gene="uidR"
                     /locus_tag="RH058_000905"
     CDS             11570..12160
                     /gene="uidR"
                     /locus_tag="RH058_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV3136530.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            12548..14359
                     /gene="uidA"
                     /locus_tag="RH058_000906"
     CDS             12548..14359
                     /gene="uidA"
                     /locus_tag="RH058_000906"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV3136531.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            14356..15729
                     /gene="uidB"
                     /locus_tag="RH058_000907"
     CDS             14356..15729
                     /gene="uidB"
                     /locus_tag="RH058_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV3136532.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            15768..17033
                     /gene="uidC"
                     /locus_tag="RH058_000908"
     CDS             15768..17033
                     /gene="uidC"
                     /locus_tag="RH058_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV3136533.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQVTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(17078..18586)
                     /locus_tag="RH058_000909"
     CDS             complement(17078..18586)
                     /locus_tag="RH058_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV3136534.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18687..19862)
                     /gene="manA"
                     /locus_tag="RH058_000910"
     CDS             complement(18687..19862)
                     /gene="manA"
                     /locus_tag="RH058_000910"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV3136535.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20061..21707
                     /gene="fumB"
                     /locus_tag="RH058_000911"
     CDS             20061..21707
                     /gene="fumB"
                     /locus_tag="RH058_000911"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV3136536.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21850..23253
                     /gene="fumC"
                     /locus_tag="RH058_000912"
     CDS             21850..23253
                     /gene="fumC"
                     /locus_tag="RH058_000912"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV3136537.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(23250..24179)
                     /gene="tus"
                     /locus_tag="RH058_000913"
     CDS             complement(23250..24179)
                     /gene="tus"
                     /locus_tag="RH058_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV3136538.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(24255..25556)
                     /gene="rstB"
                     /locus_tag="RH058_000914"
     CDS             complement(24255..25556)
                     /gene="rstB"
                     /locus_tag="RH058_000914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV3136539.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(25560..26279)
                     /gene="rstA"
                     /locus_tag="RH058_000915"
     CDS             complement(25560..26279)
                     /gene="rstA"
                     /locus_tag="RH058_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV3136540.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            26408..26743
                     /locus_tag="RH058_000916"
     CDS             26408..26743
                     /locus_tag="RH058_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV3136541.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(26740..27462)
                     /gene="folM"
                     /locus_tag="RH058_000917"
     CDS             complement(26740..27462)
                     /gene="folM"
                     /locus_tag="RH058_000917"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV3136542.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(27499..28881)
                     /locus_tag="RH058_000918"
     CDS             complement(27499..28881)
                     /locus_tag="RH058_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3136543.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(29067..30011)
                     /locus_tag="RH058_000919"
     CDS             complement(29067..30011)
                     /locus_tag="RH058_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3136544.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            30535..32067
                     /gene="pntA"
                     /locus_tag="RH058_000920"
     CDS             30535..32067
                     /gene="pntA"
                     /locus_tag="RH058_000920"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV3136545.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32078..33466
                     /gene="pntB"
                     /locus_tag="RH058_000921"
     CDS             32078..33466
                     /gene="pntB"
                     /locus_tag="RH058_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV3136546.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(33491..34525)
                     /gene="tqsA"
                     /locus_tag="RH058_000922"
     CDS             complement(33491..34525)
                     /gene="tqsA"
                     /locus_tag="RH058_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV3136547.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            34937..35302
                     /gene="mdtJ"
                     /locus_tag="RH058_000923"
     CDS             34937..35302
                     /gene="mdtJ"
                     /locus_tag="RH058_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV3136548.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            35289..35618
                     /gene="mdtI"
                     /locus_tag="RH058_000924"
     CDS             35289..35618
                     /gene="mdtI"
                     /locus_tag="RH058_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV3136549.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(35657..36478)
                     /locus_tag="RH058_000925"
     CDS             complement(35657..36478)
                     /locus_tag="RH058_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV3136550.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(36578..36661)
                     /locus_tag="RH058_000926"
     CDS             complement(36578..36661)
                     /locus_tag="RH058_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136551.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(36754..37089)
                     /locus_tag="RH058_000927"
     CDS             complement(36754..37089)
                     /locus_tag="RH058_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV3136552.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            complement(37486..38739)
                     /locus_tag="RH058_000928"
     CDS             complement(37486..38739)
                     /locus_tag="RH058_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3136553.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFNSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            38846..39739
                     /locus_tag="RH058_000929"
     CDS             38846..39739
                     /locus_tag="RH058_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136554.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            39874..41094
                     /gene="mlc"
                     /locus_tag="RH058_000930"
                     /gene_synonym="dgsA"
     CDS             39874..41094
                     /gene="mlc"
                     /locus_tag="RH058_000930"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV3136555.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            41219..41914
                     /gene="bioD"
                     /locus_tag="RH058_000931"
     CDS             41219..41914
                     /gene="bioD"
                     /locus_tag="RH058_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV3136556.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(41867..43159)
                     /gene="clcB"
                     /locus_tag="RH058_000932"
     CDS             complement(41867..43159)
                     /gene="clcB"
                     /locus_tag="RH058_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV3136557.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(43319..43933)
                     /gene="dmsD"
                     /locus_tag="RH058_000933"
     CDS             complement(43319..43933)
                     /gene="dmsD"
                     /locus_tag="RH058_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV3136558.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(43976..44830)
                     /locus_tag="RH058_000934"
     CDS             complement(43976..44830)
                     /locus_tag="RH058_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV3136559.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(44832..45449)
                     /gene="dmsB"
                     /locus_tag="RH058_000935"
     CDS             complement(44832..45449)
                     /gene="dmsB"
                     /locus_tag="RH058_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV3136560.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(45460..47883)
                     /locus_tag="RH058_000936"
     CDS             complement(45460..47883)
                     /locus_tag="RH058_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV3136561.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
BASE COUNT        12118 a        11843 c        12392 g        11600 t
ORIGIN      
        1 cgggcgatag ctgcttcaac ggcagtttta cgtggatcag caacggttgc tgcgtcgtta
       61 gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc ccgtgctgca
      121 ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg tgtagcctgg
      181 gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc tttggctgca
      241 ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg ctccagacga
      301 gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc ctgacgaata
      361 gccgcaattt cagctttttc ctgacggaaa tattgcacca ggggaatatt gctcgggcaa
      421 acccacgcgc aagccccaca ttcaatgcaa tcagcaatgt tatgcgtggt agctttatcg
      481 tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccgc agggcaggcg
      541 tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc aagttcattg
      601 gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag ccatggcaag
      661 gtaaagccca ttagcgggcc acccataatc accatttgat cggcagaggg gcagaatccg
      721 gcatcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt gcccgggcga
      781 gcgattgctt cgccagtcag ggttacaaca cgctcggtaa tcggctcgcc atcaataacg
      841 gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat atcggatgaa
      901 cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt agcaccgcca
      961 gaaggatatt tggttggaat cacccgcaga gaaatatcgt tagagtccgc cagcaccgcg
     1021 cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat ttcgcgtggc
     1081 tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca atcctgcatc
     1141 aaacggtcat cggcggtaat gtacggctcg cactcagccg cgttgataat caacgtttca
     1201 atcttatctc cgccaccctg caatttaacg cctgtcggga atcctgcacc gcccagcccg
     1261 gcaacaccaa actgatgtat gcgctcgatt aactcttcgc gactgcgagt gcgataatcg
     1321 gcccagccgt cgcgcgggat ccagcagtct tcaccatcgg catcaataat cacgcttaat
     1381 tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac ggtacccgag
     1441 gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg gccgcgcaat
     1501 actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg tttcagtgga
     1561 ataacaaaac gctgcgccag tggtacctgg cgcaggggtg taccgttgga ctgggttttc
     1621 atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct gaatgcagag
     1681 aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt tcagatccca
     1741 tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg tcgggcacgg
     1801 atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca tggcacgggt
     1861 agcgccaacg atggcgtcta ccggacacgc ctgaatacat ttagtgcagc caatacagtt
     1921 attttcatca ataaccgcca ccatccgcgc aggcgttatc tcttgcgctt cgccatccag
     1981 cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt cgccacctgg
     2041 ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg ggcgacagcc
     2101 gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt tctcaacgac
     2161 cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc caaacgccag
     2221 gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa cttcaccaaa
     2281 ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc aatggcatta
     2341 ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc ggcgaagagc
     2401 accatcacca gcgagaaacc gatagcggcg gaaaaaccgt acagcgccga ctgcaagaaa
     2461 ttgtgcccga gattgatatt cagcaacgcc acgccgagca ctgcacagtt ggtggtgata
     2521 agcggcaaaa aaatccccag cagacggtaa agcaccgggc tggttttgcg caccaccatc
     2581 tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg caggtaaatc
     2641 agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga cgccagcgtc
     2701 atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagtttttt ggaaaccccc
     2761 ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag tacagttccg
     2821 acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc gcctattatc
     2881 cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat tcacaaacgt
     2941 cgcttttacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg ccaacaaagt
     3001 aaacagcaac tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga atgcttttac
     3061 tgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac aacgccgacg
     3121 tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag cggtaataaa
     3181 agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt gaaatgtttg
     3241 cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca acagagcgac
     3301 taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg ttgtggtggt
     3361 cataggttct ccagaatagc gacgcaaaca ggcaaatcta attttagggg cgttattata
     3421 aggcttttat aaccgatcgc tactttcttt attggacata gtgccagacg gacttcggta
     3481 cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg tgatcggcag
     3541 cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga tggtcgtaga
     3601 gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag tcctgaacag
     3661 tcataaaagg tccataaaat aattaatacc aaaggggtag gtattattag tgatgataaa
     3721 atttcaccaa aagtgaacgc ttgtgttatt acaataataa gaatgcgctg atggcaaata
     3781 ttcagggatg aaaaactgct ctgaaaagag taacgcgctt tactatccct gacaatcacc
     3841 aacaacatcg aacaagataa taaattcctg gtttaatatc cgacaagtga aaacatgcac
     3901 ccggacgggc agcatgtcgc tccacaagtg cagagcttac ttgtgttgta ccgaagcact
     3961 ctgttcaggt ggctgatagt tgtcaatgtg actcgccacg ccaagaagaa tgactgaaac
     4021 gacaagaacg atccaacctg ttaattcaat aagacgattc attacagccc acatctctct
     4081 tgattgatcc attaacttca gggggtaaat gttacttagc aataatagct ccagcaagat
     4141 ttttactgag gtttttgcga tatttagctt ttgtcgttgg aaaattcgct atttttttga
     4201 cttaagttaa acaacatccc ttattgctgg caggttatta aactgttgag cgtgggtaaa
     4261 ggatagtgtc aaatagccat catacttcaa tgagaggcaa tgacatgagc gacaacatcc
     4321 gtgttgggtt gattgggtat ggttatgcga gcaaaacctt ccatgcgccc ctgattgcgg
     4381 gcacgcccgg gctggaactg gcggtaatct ccagcagtga tgaaacaaaa gtaaaagccg
     4441 actggccaac ggttacggtt gtctctgagc cgaagcatct gtttaacgat cccaacatag
     4501 acctgattgt cattcctaca cccaacgata cccatttccc gttagccaaa gcggcgcttg
     4561 aggcgggtaa acatgtggtc gttgataaac cctttaccgt gacactgtca caagcgcgag
     4621 agctggatgc gctggcaaaa agcctggggc gtgtgctgtc tgtattccat aaccgtcgct
     4681 gggatagcga tttcctgacg ctaaaaggtt tgctcgcgga aggcgtgctg ggtgaagttg
     4741 cttactttga gtctcatttt gaccgcttcc gtccgcaggt gcgcgatcgt tggcgtgaac
     4801 agggcggccc aggcagcggt atctggtacg atttagcacc acatcttctt gatcaggcca
     4861 ttacgctttt tggtttaccg gtcagcatga cggtagattt ggcacagtta cggcccggag
     4921 agcagtcgac cgattatttc cacgccatct tgtcctatcc acagcggcga gtcattttac
     4981 acggtaccat gctggcagcc gctgagtcag cacggtatat cgtgcatgga tcccgaggca
     5041 gttatgtgaa atatggcctc gatccacagg aagaacgtct gaaaaatggc gagcgtctac
     5101 cgcaggaaga ctggggctac gatatgcgtg atggcgtact tacccgcgtg gaaggtgagg
     5161 aacgtgtcga agaaacgctg ttgacggtgc ctgggaatta tccggcttac tatgcggcta
     5221 ttcgtgatgc gttaaatggc gatggtgaaa atccggttcc ggcaagccag gcaatccagg
     5281 taatggagtt gattgagctg ggcatcgaat ccgccaaaca tcgcgcgact ttgtgccttg
     5341 catgaaaaat ggatggtgca atttgcacca tccattttgt tacttcgcgg cgactttttc
     5401 tcgcagtgcg cgtttttcct cagcgctgag gaaagccatt tccagaccat taatctgtgc
     5461 ctggcggatt tgctcgcggg ataacccagc agctggcgcg gcaacggtat attcgtgaat
     5521 gatatccact ccctgtacgc cgggatcgtc agtgttaatg ctggcacgaa tgccatgctc
     5581 aaggaacgtt ttcagcggat gtgcagccag ctctgctacg gtgctggtct gaatattgga
     5641 ggtcagacag gattcaatac caatttgttg ctcggcgaga aaatccatca gcgcccgatc
     5701 ttcaatggct tttacgccat gtccaatacg ctccgcaccc agttcacgaa tcgcctgcca
     5761 gatgctttcc ggcccggcag cttcgcctgc atggacggta atatgccagc ccgcatcacg
     5821 cgcgcggttg aagtgagaaa ggaacagact tcccgggaaa ccaagttcat caccggctaa
     5881 atcaagtgcg gtaatctggt cacggtgggc taaaaaggcc tccagctctt gctgacaggc
     5941 ggcttcgccg aaggtccggc tcataatgcc gataagcttc gcctgcacac caaaggtgcg
     6001 gcaaccttca cgtacgccat cgatcaccgc ttcgacaaca cccgctacag gcagctgatg
     6061 tgccattgcc atgtagcctg gtgaaaaacg cagctcgaca tagtgcaggc cgtgacgggc
     6121 tgcatcttca atgttttcaa atgccacgcg gcgacaggca tcaagagagg cgagaacttt
     6181 aacgccccag tcaagtttgg tcagaaagct caccagatcg ggttcgttgg caatgacctg
     6241 aacgtgggga atcagtgttt ccagggattg tgcaggaagc gagatattat actggcggcc
     6301 aagttcaaga atggtctggg gacgaatgtt gccatcaagg tggcgatgga tatcagttaa
     6361 tggcagggtg gtatcaatca tggtcgcact ctttttctga atataaaagt gcgcaccatt
     6421 ataaaaacaa agcagggtaa aaatccacga ttgcgcaacg attttttacg ttgcgcaatt
     6481 ggggttagcg aacagcgcgg atggcgttta ttaatccagc cacacctttt tccagtttcg
     6541 aacgtgggca gccggcattg agacggacaa aaccacgacc ttcttcacca taggtatacc
     6601 ccggcatgat cgcgactttt tcttgctcga taagcgcttt ttgcaacgcg ttgtcgtcaa
     6661 tattcaacgg acgtagatca agccaggcca gataagtgga ttgcgggatc tgccagttga
     6721 gttcaggaaa cgcggcgttc attttatctg agatatacgt caggttatct ttcagataga
     6781 cgcgtaaggc atccagccac ggtgcacctt gctgataggc ggcgatatgg gcagttaacg
     6841 ccagtaccga aggggaagaa agcccatcac gtcctttcag tgccgataaa taggcatcac
     6901 ggctgctgct attttctata atcccgtaag caccggtcag cgcgggaata ttgaaacttt
     6961 tcgagcccga cgtcagcaac gcccagtctc cccgcgccac attactccag ggaatatgcg
     7021 gctgctcgcc ccagaccata tccatatgga tttcatcgga aataacccgc acaccatgac
     7081 gctcgcacag gtcagccatg atctccagct catcgcacgt ccacactttc ccggtaggat
     7141 tctgtgggct acacaggagc ataattttac attctggttt cgccaacacg gcttccagct
     7201 tgcccatatc gcaaaaccag tcgtcagcct gcttctctaa agcaacgggc attactgtgc
     7261 gctggttacc ttcaatggcc ttgtaaaatg cgtcataggc gggcgtgtgg atcaccacgc
     7321 cttcacctgt ttcagaccac tgacgaatca gttctgaaac catatagatg acagaagggc
     7381 catacaccac cgtctgcgta tcaattgcgg tgtaatgctg ggtggaaaac cagtgggcaa
     7441 tagccgcgag aaactcatcg tttttccagc ggctgtagcc aaatacgccg tgcatcaggc
     7501 gctgattcag cgcctcgata atgcaggggg cagtggcaaa atccatgtct gaaatcgtga
     7561 acggtaacag gtcagcagtg ccgaaacggt cagcgacata atcccactgt gtacaccatg
     7621 tgccatgacg atccacgacc tttgaaaaat cgaacatatc ttatccttat gcccggacag
     7681 tatgcatcag accggccatt tcatctttta ctgactgaac ttgtggcccg ataacaacct
     7741 gcaggttatg ttgattaagt tgtactacgc caattgcccg attgtccttc agtgcctgca
     7801 cattaacaag cgacatatct ttcacagaca aacgcagacg ggtaatgcag ttatcgaggc
     7861 tgacaatatt gtcggcaccg cctaatgctt cgaggattgc aggaacgttg taacctgatt
     7921 tacccggcgc accggcaacg gctttttcga ttgagctggc aacttcgcta tcgcgccccg
     7981 gggttttcag attgaagcgg gtgatagcga aacggaagat gacgtagtaa acgacaaacc
     8041 agattgccgc caccactggc accatgtacc acttggttga cagaccatgc aaaataccga
     8101 acaccacgaa gtcgatgata ttgccgtcgg tattaccgat ggtgacgccg agcacagaca
     8161 tgacagtgaa gccgaggccg gttaacagcg cgtggatgac atacagaact ggcgccacga
     8221 acaggaacag gaattccagc ggttcggtcg tgccgccaac gacacaggcg atcaggccag
     8281 aaatcagcag acctttaatt ttatggcgat tttccgggcg cgcgcagtga tacatagcta
     8341 acgctgcacc tggcagaccg ccgagaaacg caggcatttt accttgcgaa aggaaacgcg
     8401 tggcgctttc agaaaaaccg tgagtggtcg ggcaactcaa ttgcgcctgg aagatggtca
     8461 gtgcgccgct gacggtttga ccgcagactt cctgcgtgcc gcctgcgtcg gtaaagcgaa
     8521 ttaatgccac cagaatgtga tgcagaccaa acggcaacag cagacgttca ccggtaccaa
     8581 acagcatcgg tccgaaatca cccgcgctgt ttatcatatg gcccaagccg ctaataccca
     8641 tggcgaaaat cggccagact aatggaatca ccaggccgat aaggcccatc accagcgagg
     8701 agataattgg tacgaagcgc gtaccgccga agaatgccag cgcatccggc aggcggatat
     8761 tatggaaacg ctcatgcagc atccagacga taataccggc gatcaccgca ccgaggatcc
     8821 cggtatcgat cgactggatc ccaagaatgc tctggatgtt attggctttc agaaccgcgg
     8881 catccgtggt tggcagaatg cctttattgg tcaaccagaa gtttaccgcg aggttcatta
     8941 ccgcataacc gacgaagcca gcgaatgccg ctacgccttt attttcgcgt gccaggccca
     9001 gcgggatggc gatacagaac atgacaggca ggaaactaaa agcaaacgag ccaatcttac
     9061 tcatccaggt aaagatagcc tgcaacacgg ggttgcccag gaccgggatc agggttatga
     9121 catcatggct gctaagagaa ctaccaatgc cgagcataat gccgcagaac gacaataatg
     9181 ccacgggtaa catgaaggtt ttgcctaact gctggaagaa ctcccacagc gtgactttcg
     9241 gtgctgtttt cgccgtcata aaacgactcc tcgtagagaa caatctggat tcagtaaatt
     9301 gcgcgagaag ataaaacgtt ttatcaaatt ttagtgaggc ataaatcaca ttacgcaacg
     9361 ataatagcgg gtataagata aataaaaggt aaaacgtttt atctgtcaca taatcaggga
     9421 gtaggtcatc tgcatggcta ccgccaaaaa aataaccatt catgatgttg cgctggctgc
     9481 gggcgtgtcg gtaagtaccg tttcgctggt gcttagtggc aaagggcgaa tctctaccgc
     9541 cacaggagaa cgcgttaacg ccgccattga agagctggga tttgtgcgca atcgccaggc
     9601 gtcggcgctg cgcggcgggc aaagcggcgt cattggtttg atcgtccgtg atttatctgc
     9661 gccgttttac gccgaattga cggccggatt gacggaagct ctggaagcgc agggacggat
     9721 ggtttttttg cttcacggcg gtaaagacgg cgagcagctg gcacagcggt tttcactgtt
     9781 actgaatcag ggtgtggatg gtgtggtaat tgccggggct gcaggaagca gcgatgacct
     9841 gcgacggatg gcagaagaaa aagctatccc ggtgattttc gcttcccgtg ccagttatct
     9901 tgatgatgtt gatacggttc gcccggacaa catgcaggct gcacagttgt tgacggagca
     9961 tctcattcgc aatgggcatc agcggatcgc ctggctggga gggcaaagtt cctcattaac
    10021 ccgtgcagaa cgggtggggg gctattgtgc aactctacta aaatttggcc tgccgtttca
    10081 cagcgattgg gtgctggagt gcacatccag ccagaagcaa gccgcggaag ctatcacggc
    10141 gcttttacgt cataacccga ccatcagtgc tgtggtttgc tataacgaaa ctattgcgat
    10201 gggggcatgg tttggtttgc tcaaagcagg gcggcaaagc ggggaaagcg gagtcgatcg
    10261 ttactttgag caacaagttt cgctggcggc atttaccgat gcgacaccaa ccacacttga
    10321 tgatataccc gttacctggg ccagcacgcc tgcgcgggaa cttggtacca cacttgcgga
    10381 tcgcatgatg caaaaaatca cccatgaaga gacgcattca cgcaatctta ttattcccgc
    10441 ccggctcatt gcggcgaaat aatcctctct ttatctgcta tacctggtag tgtcctttcc
    10501 tcaaggttaa tgataaaaaa tgtggcacac aggcggtgcc ttttattttt actgaagttc
    10561 acaggaggtt tatgtgttta attctgacaa cctgagactc gacggaaaat gcgccatcat
    10621 cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt acattcgcga cagctggcgc
    10681 atctgtggtg gtcagtgata ttaacgccga cgcagctaac catgttgtag acgaaattca
    10741 acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt acttccgaac aggaactctc
    10801 tgcactggca gactttgccg tcagtaagct gggtaaagtt gatatcctgg ttaacaacgc
    10861 cggtggcggt ggtcctaaac cgtttgatat gccaatggca gattttcgcc gtgcttatga
    10921 actgaatgtg ttttcttttt tccatctgtc acaacttgtt gcgccagaaa tggaaaaaaa
    10981 tggcggtggc gttattctga ccatcacttc tatggcggca gaaaataaaa atataaacat
    11041 gacttcctat gcatcatcta aagctgcggc cagtcatctg gtcagaaata tggcatttga
    11101 cctgggtgaa aaaaatattc gggtgaatgg cattgcgccg ggggcaatat taaccgatgc
    11161 cctgaaatcc gttattacac cagaaattga acaaaaaatg ttacagcaca cgccgatcag
    11221 acgtctgggc caaccgcaag atattgctaa cgcggcgctg ttcctttgct cgcctgcagc
    11281 cagctgggta agcggacaaa ttctcaccgt ctccggtggt ggggtacagg agctcaatta
    11341 atacactaac gaaccggtaa acagccgtat gtgttgttta ccgggatagc gtgtgcaatt
    11401 catcaacgtc tctgctaaac aactggcagc caaatcacgg ctattggtta accaatagct
    11461 ggatgaaaag catacgaata gagtgtgcct tcgcactatt caacagcaat gataggcgct
    11521 cacctgacaa cgcggtaaac tagttattca cgctaactat aatggtttaa tgatggataa
    11581 catgcagact gaagcacaac cgacacggac ccggatcctc aatgctgcca gagagatttt
    11641 ttcagaaaat ggatttcaca gtgcctcgat gaaagccatc tgtaaatcct gcgccattag
    11701 tcccgggacg ctctatcacc atttcatctc caaagaagcc ttgattcagg cgattatctt
    11761 acaggaccag gagagggcgc tggcccgttt ccgggaaccg attgaaggga ttcattttgt
    11821 tgactatatg gtcgagtcta ttgtctctct cacccatgaa gcctttgggc aacgggcgct
    11881 ggtggttgaa attatggcgg aagggatgcg taacccacag gtcgccgcca tgcttaaaaa
    11941 taagcatatg acgatcactg aatttgttgc ccagcggatg cgtgatgccc agcaaaaagg
    12001 cgagataagc ccagacatca acacgtcaat gacttcacgt ttactgctgg atctgaccta
    12061 cggtgtactg gccgatatcg aagcggaaga cctggcgcgt gaagcgtcgt ttgctcaggg
    12121 attacgcgcg atgattggcg gtatcttaac cgcatcctga tcctctcatt tcgcgggctg
    12181 gtgataactg tgcccgcgtt tcatacttta atttccctgt acaaaataca atctagccgg
    12241 gatcggagaa ttccccccaa aaatattcac cgtagccata tgtcatgaga gtttatcgtt
    12301 ctcaagatgc tcgaacgaac gttcgattgc ttattttatg gcttctgtca acgctgtttt
    12361 aaagattaat gcgatctata tcacgctgtg ggtattgcag attttggttt tttgatcgcg
    12421 gtgtcagttc tttttatttc catttctctt ccatgggttt ctcacagata actgtgtgca
    12481 acacagaatt ggttaactaa tcagattaaa ggttgaccag tatttttatt tgaatgagga
    12541 gtcccttatg ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct
    12601 gtgggcattc agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc
    12661 gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc
    12721 agatattcgt aattatgtgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg
    12781 ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg
    12841 ggtcaataat caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt
    12901 cacgccgtat gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact
    12961 gaactggcag actatcccgc cgggaatggt gattaccgat gaaaacggca agaaaaagca
    13021 gtcttacttc catgatttct ttaactatgc cgggatccat cgcagcgtaa tggtctacac
    13081 cacgccgaac acctgggtgg acgatatcac cgtggtgacg catgttgcgc aagactgtaa
    13141 ccacgcgtct gttgactggc aggtggtagc aaatggtgat gtcagcgttg aactgcgtga
    13201 tgcggatcag caggtggttg caactggaca aggtaccagc gggactttgc aagtggtgaa
    13261 tccacacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag
    13321 ccagacagag tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg
    13381 cgaacagttc ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga
    13441 tgctgacttg cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat
    13501 ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct
    13561 cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa
    13621 cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga
    13681 ggcagtcaac ggggaaactc agcaggcgca cttacaggcg attaaagagc tgatagcgcg
    13741 tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc
    13801 gcaaggtgca cgagaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac
    13861 gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga
    13921 tctctttgat gtgctgtgcc ttaaccgtta ttacggttgg tatgtccaaa gcggcgattt
    13981 ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca
    14041 gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac
    14101 cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga
    14161 tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca
    14221 aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa
    14281 gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca
    14341 gcagggaggc aaacaatgaa tcaacaactc tcctggcgca ccatcgtcgg ctacagcctc
    14401 ggtgacgtcg ccaataactt cgccttcgca atgggggcgc tcttcctatt gagttactac
    14461 accgacgtcg ctggcgtcgg tgccgctgcg gcgggcacca tgctgttact ggtgcgggta
    14521 ttcgatgcct tcgccgacgt ctttgccgga cgagtggtgg acagtgtgaa tacccgctgg
    14581 ggaaaattcc gcccgttttt actcttcggt actgcgccgt taatgatctt cagcgtgctg
    14641 gtattctggg taccgaccga ctggagccat agcagcaaag tagtgtatgc atatttgacc
    14701 tacatgggcc tcgggctttg ctacagcctg gtgaatattc cttatggttc acttgctacc
    14761 gcgatgaccc aacaaccaca atcccgcgcc cgtctgggcg cggctcgcgg gattgccgct
    14821 tcattaacct ttgtctgcct ggcatttctg ataggaccga gcattaagaa ctccagcccg
    14881 gaagagatgg tgtcggtata ccatttctgg acgattgtgc tggcgattgc cggaatggtg
    14941 ctttacttca tctgcttcaa atcgacgcgt gagaatgtgg tacgtatcgt ggcgcagccg
    15001 tcattgaaga tcagtctgca aaccctgaaa cggaatcgcc cgctgtttat gttgtgcatc
    15061 ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt gttctacgtg
    15121 cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca aaacctggtt
    15181 ggtactgtgg catcggcacc gctggtgccg gggatggtcg cgaggatcgg taaaaagaat
    15241 accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctattctt ctgggtttcc
    15301 gtctggtcac tgccggtggc gttggttgcg ttggccatcg cttcaattgg tcagggcgtt
    15361 actatgactg tgatgtgggc actggaagct gataccgtag aatacggtga atacctgacc
    15421 ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa atgcggtcag
    15481 gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat cgccaatcag
    15541 gtgcaaacgc cggaagttat tatgggcatc cgcacatcaa ttgccttagt accttgcgga
    15601 tttatgctac tggcattcgt tattatctgg ttttatccgc tcacggataa aaaattcaaa
    15661 gaaatcgtgg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat cagcgatatc
    15721 actaattaat attcaataaa aataatcaga acatcaaagg tgcaactatg agaaaaatag
    15781 tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct tatgcggcgc
    15841 aactggctga cgatgaagcg ggactacgca tcagactgaa aaacgaattg cgcagggcgg
    15901 ataagcccag tgctggcgcg ggaagagata tttacgcatg ggtacaggga ggattgctcg
    15961 atttcaatag tggttattat tccaatatta ttggcgttga aggcggggcg tattatgttt
    16021 ataaattagg tgctcgtgct gatatgagta cccggtggta tcttgatggt gataaaagtt
    16081 ttggctttgc cctgggggca gtaaaaataa aacccagtga aaatagcctg cttaaattag
    16141 gtcgcttcgg gacggattat agttatggta gcttacctta tcgtattccg ttaatggctg
    16201 gcagttcgca acgtacatta ccgacagttt ctgaaggagc attaggttat tgggctttaa
    16261 caccaaatat tgatctgtgg ggaatgtggc gttcacgagt atttttatgg actgattcaa
    16321 caaccggtat tcgtgatgaa ggggtgtata acagccagac gggaaaatac gataaacatc
    16381 gcgcacgttc ttttttagcc gccagttggc atgatgatac cagtcgctat tctctggggg
    16441 catcggtaca gaaagatgtt tccaatcaga tacaaagtat tctcgagaaa agcataccgc
    16501 tcgacccgaa ttatacgttg aaaggggagt tgctcggctt ttacgcgcag ctcgaaggtt
    16561 taagtcgtaa taccagccag cccaatgaaa cggcgttggt tagtggacaa gtgacctgga
    16621 atgcgccgtg gggaagcgta tttggcagtg gtggttattt gcgccatgca atgaatggtg
    16681 ccgtggtgga taccgacatt ggctatccct tttcattaag tcttgatcgt aaccgtgaag
    16741 gaatgcagtc ctggcaattg ggcgtcaact atcgtttaac gccgcaattt acactgacat
    16801 ttgcaccgat tgtgactcgc ggctatgaat ccagtaaacg agatgtgcgg attgaaggcg
    16861 cgggtatctt aggtggtatg aactatcggg tcagcgaagg gccgttacaa gggatgaatt
    16921 tctttcttgc tgccgataaa gggcgggaaa agcgcgatgg cagtacgctg ggcgatcgcc
    16981 tgaattactg ggatgtgaaa atgagtattc agtatgactt tatgctgaag taaaaaataa
    17041 cgccggagag aaaaatctcc ggcgttttgt ctgtgaatta ctgctgcgga atagcgggta
    17101 cgaccggaac atttaatgcc ggcattgcaa acataccaac gaaatcttcc agtggcattt
    17161 tctgcccgtt taacgttatc tgaccgttag tatattgcag gctggtggtg atggtattgt
    17221 cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca acttgctgtt
    17281 tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc tgagtcataa
    17341 actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta accgaacgat
    17401 ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct ttcaggaaca
    17461 gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc gcgatagtaa
    17521 tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc gtcactttct
    17581 cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc tgagtttgcg
    17641 cgttatattg ctggctaaac tgatgccagg cttcaccatc aatctggccg acttttaaag
    17701 tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc gagtaatcca
    17761 gttggctatt gatcgtttta ccgtcattga ccagatccga tttaccgctg atctccatgc
    17821 cttccagcag tgccagttct ttgccttcca ccgaaatggt cattttttcc agtgacagtt
    17881 tttggtttcc tacgcgctca ccaaaacttg ccagcgtgct ggaaccgtcg gttttcagat
    17941 tattaaaggt caactgcact ttctggttgt attcgttaac tgcgtctatc cgaccacttt
    18001 gcgcctcccc ggaaagggag atagctttgc cgtctctgtc cgcatttaac tggaactcgc
    18061 cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggcttg agcgaaatat
    18121 cggaactgga atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc gtttcacctt
    18181 ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt tgaattgatg
    18241 ccatcgacgg gatcaggttc agttttttaa gctgggcaag cgggaaggga ccatgatcaa
    18301 ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggattttct ttcccggcaa
    18361 tgggtttcac caacagttgc aactggctgc tgaatacgcc gcgatgatag ttttgataac
    18421 tcacttccag gttggattca ggagcggtca gtttgagttg cgcgttcgcc tgcgcgacca
    18481 tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct gtccagacta
    18541 cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg attatccata
    18601 aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata agcttagcaa
    18661 gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc taaacggccg
    18721 tggcctttga cagtcaccgg tgattcgttg gcggcaataa acgctgattc acccggttta
    18781 agctgtaact gctgagaacc tttccacaac gttgcatcgc cttcgacgca gaacaaaatg
    18841 gcggcactct gctggctaat ggtggtttct ttatcactaa ggtcatgcag cgagaaggca
    18901 aaatcatcca ctggaatcgg gaagtccagt tctgcacctt gtttcaccgg ctgggtcaac
    18961 aactggttag ccggtttggc ttcgaatttc acattggcaa ccagttccgg aatatcaatg
    19021 tacttaggcg tcagacccgc acgcagcacg ttatcggagt tcgccatcac ttccagcgcc
    19081 acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc gccaggattc
    19141 aatttcacca cattcagtaa cagcggggag aacaggccgc tgtcttccgg gtaaaattca
    19201 gaaattaaac gaatcgtttg ccacggttca ccctgctgac tgtctagcgc cgattttaaa
    19261 atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggctggc gaacagttcg
    19321 cttaaacgtt cggcattagg ctgttgtaaa aagtgagcaa ttgccggatg tgcacctgcg
    19381 actggctgga gtagggagac aatctcggaa aactcacgaa acgcgttcat cgcaaggaaa
    19441 ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt acgctcggcg
    19501 gcatccatcg ggatacctgc ggcattttct ttggcaaaac cgatttcaga attgcgtttg
    19561 tttggatgaa cctgaatgga gagcggctgt gctgcgcata atactttgaa caggaaaggc
    19621 agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatt actctcaatc
    19681 acatcacgca gtgaaacgat atctccggcg gcattctgca cccgtgaact gcttttcgga
    19741 tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat accataaagt
    19801 tcagtcaacg ccgttttgct gccccaggca tagttttgca ctgagttaat gagtttttgc
    19861 atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag aatcctacgg
    19921 gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa aaagtgtgta
    19981 ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca taaccaaacc
    20041 aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcaggct ccttttccac
    20101 tcaaaaaaga tgatactgag tattacctgc taaccagcga acacgttagc gtatctgaat
    20161 ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactctgttg gcgcgtcagg
    20221 catttcatga tgcgtcattt atgctgcgtc cggctcacca acaacaggtg gccgacattc
    20281 tgcgtgaccc ggaagccagc gaaaatgata aatatgtggc gctgcaattc ctgcgcaact
    20341 ccgacatcgc ggcgaaaggt gttctgccaa cctgtcagga taccggcact gcgattattg
    20401 ttggtaaaaa agggcagcgt gtatggaccg gtggctgtga tgaagcggcg ctggcgcgcg
    20461 gtgtctataa cacttatatc gaagataatt tgcgctactc gcaaaacgcg ccgctggata
    20521 tgtataaaga ggtgaatacc ggcaccaatc tgccagcgca gatcgatctt tatgccgttg
    20581 atggcgacga gtacaaattc ctctgtatcg ccaaaggtgg tggttcggca aacaagacgt
    20641 atctctatca ggaaaccaaa gcgttactaa cgccggggaa actgaaaaat tacctggttg
    20701 agaagatgcg cacgctgggt acggcggcct gtcctccgta tcatattgcg ttcgtaattg
    20761 gtggaacttc tgcagaaact aaccttaaaa cagtgaaact ggcttccgct aaatactatg
    20821 acgaactgcc aacggaaggg aatgagcacg gtcaggcatt ccgcgatgtg gaactggaaa
    20881 aagaattgct gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt ggtaaatact
    20941 tcgctcacga catacgcgtg attcgcctgc cacgtcacgg cgcatcctgt ccggttggta
    21001 tgggtgtttc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt caggggatct
    21061 ggatcgaaaa actggaacat aatccaggta aatatatccc tgaagagctg cgtaaagcgg
    21121 gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc ctcgcacagt
    21181 tgtcgcagta tcccgtttct acacgcttat cgcttaacgg cacgattatc gtcggtcgtg
    21241 atattgctca cgccaaactg aaagagcgga tggataacgg tgaagggctg ccgcagtaca
    21301 ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt tatgcctccg
    21361 gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa ctgcaagcgc
    21421 agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg acggatgcct
    21481 gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggccgct gtattggcgc
    21541 agggaagtat taagagcctg gaatgtgttg aatatccgga actgggaatg gaagccatct
    21601 ggaaaattga agtggaagat ttcccggcgt ttatccttgt ggatgataaa ggaaatgact
    21661 tcttccagca gatacaactc acacagtgca ctcgctgtgt gaaataaaca gagccgccct
    21721 tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa tagtaaataa
    21781 taagcgagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta aacaggtgag
    21841 gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggggcgat tgatgtcccg
    21901 gcagataagc tgtggggcgc acaaactcaa cgctcgctgg agcatttccg catttcgacg
    21961 gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc agcggcaaaa
    22021 gttaatgaag atttaggctt gttgtctgaa gagaaagcga gcgccattcg gcaggcagcg
    22081 gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg gcagaccggc
    22141 tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc cagtgaatta
    22201 ctcggcggcg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt gaacaaaagc
    22261 caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcgctgct ggcgctgcgc
    22321 aagcaactca ttccgcagct taaaaccctg acacagacgc tgagtgaaaa atcccgtgca
    22381 tttgccgata tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc gctaacacta
    22441 gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctcaaaca tatcgaatac
    22501 agcctgcctc acgtagcgga actggctctt ggcggtacag cggtgggtac tggactaaat
    22561 acccatccgg agtatgcgcg tcgcgtagca gatgaactgg cagtcattac ctgtgcaccg
    22621 tttgttaccg cgccgaacaa atttgaagcg cttgcgacct gtgatgccct ggttcaggcg
    22681 cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga tgtccgctgg
    22741 ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa tgagccgggc
    22801 agctcaatca tgccggggaa agtgaatcca acacagtgtg aggcattaac catgctctgc
    22861 tgtcaggtga tggggaacga cgtggcgatc aacatggggg gcgcttccgg taactttgaa
    22921 ctgaacgtct tccgtccaat ggtgatccac aatttcctgc aatcggtgcg cttgctggca
    22981 gatggcatgg aaagttttaa caaacactgc gcagtgggta ttgaaccgaa tcgtgagcga
    23041 atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac ccacattggt
    23101 tatgacaaag ccgcggagat cgccaaaaaa gcgcataaag aagggctgac cttaaaagct
    23161 gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt acggccagaa
    23221 cagatggtcg gcagtatgaa agccgggcgt taatctgcaa catacaggtg cagccgtgga
    23281 atgatcaaac gaagcggctg cgcctgtggt ttataacggt gctgcacatt gtcggcatcg
    23341 taatttaaca actcaccaac gtccggcacg cccgcgccat tatcccgatt aatcagtgca
    23401 atcagtggtg tagggcaggc gtgttggact tgtttttgat ctcctttgta ccagacgcgg
    23461 gcaatcggct gcaccttcac cggacgtttg atttttaact tcgcgttctg tggcagggca
    23521 gcgatatcct gatactctcg ctccagtttt ctttgccact cctcgcgcgt ccacggtgcg
    23581 acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc acgatgcaaa
    23641 ttcttaatga tatgtttatt agcccaacca aagcgtaaag tggcggggtc gtgcagaacg
    23701 gtgagcgtgc ggtaagcatt aagggtgatc agccccggca aatgacgatg cacccattca
    23761 aaacgtgccg cggtggggag ttctgattca accgtgacga tatgctcgaa cgtggtcttg
    23821 agtttattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt atcgacctgg
    23881 taacacaaca cgccaggcag acggacagcg gccttgctgc tgcgattttc ggactgttgt
    23941 tgaataaata aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt gccgagatgt
    24001 tgttttacct caatacgatt aagcggatta tgctcatcct ctttttttac ctccggcaaa
    24061 gagaacacgc gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa tgcagccagc
    24121 tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta acgcgccata
    24181 atattaacca ctttagttac aacatactta ttttatagca cagtcgtggt gacttgacca
    24241 tcgtgcgcgt agtgtcaggc agaggtaaat tgcgggatgt tatgccataa cggccagcta
    24301 aacgagaagc gagcaccacc cagttcgctg gtgtcacaat taaccgtacc gcccattgcc
    24361 agtgctatag agtggacaat tgccagcccc agcccgcagc cgccggttga gcgatcccgg
    24421 ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg ggcaatccct
    24481 gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag gctggtttca
    24541 accgttgaat ggcagtaccg cagggcgtta ttgagcaaat tatccagcac gcgctccatt
    24601 aagcgcatat ccaacgccgc ataatggcct tgcgcgagcg ttttaatccg taccgtttta
    24661 tcgggcgtta ctgcctgaat gtctgccaga tgcgttgaca gccacaacgg caggtctggt
    24721 tcgctaagat gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag cagctcttca
    24781 attaaagctt caagttgact gatatcacga ttcaacgcct gggattcggc ggcgctcagg
    24841 ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag ttcgtgagcg
    24901 ataccgtcaa taagctgttt tttgctggca atcaaggcgt tgatattgtc cgccatctgg
    24961 ttaaatgcga taccaagtcg ttcaaagctc gaaccctcat caaagtggat acgctcattg
    25021 agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc ctgccagtgc
    25081 ggacgcatcc agataaacac cggaaaggcg agggaaatag caataaaagc gatcagggcg
    25141 atatccagca atcgcatctg atggagataa taaagataag gaacaggacc aactgccagc
    25201 acgtagtggc tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag ggcgacaatt
    25261 tcgccgccac gcagtcggtg catggaaata tcatcaagat ggtatttact cagtggctcg
    25321 acacgcagat cgaaagagag atttaaatcc atctctttca gcgtcttacc ccagtcgtgt
    25381 ggggggatct cacgcaattc gctgcgcatc agatacagcg aactgttcat caaatcatcc
    25441 agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac cagcagagac
    25501 atcacaagga agcagacaaa caataacagg taaaactgga taaacagttt tttcatcgct
    25561 tattcccatg catgaggcgc aaaaagatag cctttgttac gcacagtttt aatgcgataa
    25621 ggttctgcgg cgttatcgag cagttttttt cttaaccgcg aaatagccac gtccacgcta
    25681 cgatccagtc cgtcataact gacgccgcgt aaatttttca gcaatgcatc gcggtccatg
    25741 atttgcccgg catgggtagc taattcccac aataattcga aatcagctgt cgagagcgag
    25801 atttcagtgt tagccagggt gactacgcgg ttgatgggat cgatggtcaa cgtgccgaaa
    25861 tgcagggctt tgtagggagt cagagacgtt tcctgaagac ctttggtcag tgtggcttgc
    25921 tcattctgac gcaaatgcaa acgtaaacgc gctagcaaaa cagcaggggg cgtcgttttg
    25981 agaatatagt cgcaggcacc catttccagt gccaggatgt ggttcatatc gctatcgaga
    26041 gaggttagaa gaacaatcgg tccagaccac tttgcgcgta aatcacgaca aatggtcatg
    26101 ccgtccttgc ctggtagcat gatgtcgagt aacaccaaat ccggactttc tcgcaaaatg
    26161 gtttcttcgg cctggtcgcc gcgcggttct acggtaacct gcatatcatg ttttgccagg
    26221 tacgcggcaa tcagtgaacc gacttccgca tcatcttcca caaatacgat agtgttcata
    26281 acattcacgg tagatataaa aacgtcacaa tacaccgcgc tgtttttact tactatcaat
    26341 cgtttctaaa caagttttca tcaggtattc tactgatgac ttgttatgga agtgttaagg
    26401 taaaaagatg gggctggtaa tcaaagccgc gctcggtgcg ctggtagtgc tgttgattgg
    26461 tgttttagca aaaacgaaaa attattatat cgccgggctg attccacttt tcccgacctt
    26521 tgcgcttatc gcgcattata ttgttgccag cgaacgcggc attgaagcct tacgcgcaac
    26581 catcattttt agtatgtggt cgattattcc ctattttgtt tacctggtgt cgctgtggta
    26641 tttcaccgga atgatgcgct tgcccgccgc ttttgttggt tctgttgcgt gttgggggat
    26701 aagcgcatgg gtgttgatta tatgctggat aaagctgcat taacgcagat gacgaccgcc
    26761 atcaagtggg aaactgcgtc cggtgacaaa gcagctggta agtaagtaat cgaccaggtc
    26821 gatcacttct ttctcgccag gcgcggtttt catcagtgat ttattcagcg cctgttgtcg
    26881 atattcggca tcatcatgtt cattaaacag gatcagcgat ggcgcaatag aattcacttt
    26941 cacttccggt gccagcttgc gggcaaacga gcgggtcata ttatccagtg ccgctttgct
    27001 tgcagcatac gcaacatgtt tgtcgctacc gcgctccacc acataatcgg taaagtgaat
    27061 gatatcgctg gcggcgtgtc cgtgcccacg cagtaatctt tccagcgcat ggttgagcag
    27121 gtatggggta ttaacgtgga tctgcatcat gcaagccagt acgtcggcca gtggtgtacc
    27181 tggtttttcc gccatccacg cactggcgtt atgcaaaata gcacgcagac catgggtgct
    27241 ttttagtact tcatcggcaa acgccatcac accgtcgttg gtcgaaaaat cagcctgaat
    27301 acactgcgca cctgccttaa tcagtccatc aatggctgga tagtgcgtcc gatagctgac
    27361 aatcaccggt tgcttttgat taatgaaatg ccaggcgagg gcgaggccga tgcgacgacc
    27421 tccgccagta attaatattg gcaagggctg ggctttaccc atcgttatct cctttgctat
    27481 ccaacgatgg gatgaacgtt atcccaccag catccaggtc gccggaactg aggcaattaa
    27541 cagcatgcca atcagcacca tttcctgacg gttcagtaca ttatcatgtg tatgcgtttt
    27601 gcgcgcatac aggaaaacca gcagccccgg cgcatacaga acaacggata acagcaagtg
    27661 cattggacca gaggcataca ataaccataa gccataaatg caggcaccga cacctaccgc
    27721 tttatgcagt ggacgggttg cgattttcag caagaacgcg ccgaccagga aatagggcac
    27781 cagaatcatt tctgaggcga tggtcagcaa cgtgttgtaa tcggaaccgg tcagccagat
    27841 gagtaccaga caaatttgca cgcagatgtt ggtcaaccac agcgaggcgg atggcgcagc
    27901 ttgtgcgttc tggcgcgcaa aaatgcgtgg gaatgcttta tgagtggcag ccaggaacgg
    27961 aacttctgcc gccatgatgg tccagcttaa gtacgcgccg caaacggaaa cgatcaaacc
    28021 ggcagcgatg atgatttcgc cccatggtcc catcatttcc accatcagac cggccattga
    28081 cgggttacga atttcagcca gttcaggacg tgccaccacg cccagcgaaa gcagcgttac
    28141 cagcaagtaa acgcccagag cggagagaac cgccagcagt gtcgctttgc caacatcacg
    28201 tttattacgc gcacgcgcag aaacaaccac ggcaccttcc acaccaatga aaacccacag
    28261 ggtgatcagc atggtgtttt tcacctgttc ccacacgggt acgccaagtg caagtccggt
    28321 gaagtcgagc ttgaaggtat ccagtttgaa catcatcatc gccagcacaa caaacagacc
    28381 cagcggcaac aattttgcca gtgtcgccac caggttaatg ctggcagcgg tttgcacccc
    28441 gcgcaggatc aaaaagtgaa caatccacaa taaagccgat gcaccgacta tcgattgcca
    28501 ggtattacca tcgccgaaca ggcgcaattc cggcgtgtcc gtaaaaaagc ttaacgcgga
    28561 aaaaacgatc accagatagg agacgttggc gatgactgcg cacagccagt atccccatgc
    28621 ggaacaaaag ccgattagct cgccaaaccc ttcgcgggca taggtaaaga taccgccgtc
    28681 aagttcggga cgaatgcgcg tgaggatcag catggcaaag gccagcaata aaatgccagc
    28741 gccagtaata ccccagccga tgagcagtgc tgccgggctg gcaactgccg ccatattttg
    28801 cggcagactg aaaacacccg cgcccagcat tgagcttaat accagcgcgg tgagtgcgct
    28861 cagtcccagt ttcttttcca tcgatatcca gtgattaaaa cgtgataaga agaataaata
    28921 ttagggcaaa acgcataaaa atggagtttg ccactgaagc gcgggatttt acgaagagat
    28981 gtgtctgcat gcaatggtcg cggggaaatt tgtgactaaa aatgcgaaag gcggcatcaa
    29041 gccgccttat aggagtaact gaatagttat ttatacagat ctgcgctgac ggtcaggtta
    29101 ttaccacgtt cctgccactg gcgggtgatg tggtaatact tagcaccttt cttcgcggca
    29161 cgtttcgcaa cctgatagga gacttcggtc atgttgccgt agttgccaga gaatttgatg
    29221 ctgtcgaacg ggaccatcat cgctgctgtc gctttgttca gttcttcgac tttagtgccg
    29281 tcaggaagcg tgacagtgta acgtccgcct tttgatgact gggtttcaaa gaaacgaccg
    29341 acttcagaac ttggtgatgc ggtagtcgca acacctggga tctcaacttt cttcgcggct
    29401 tcgccaccgg cagccagagc tgcacgtcct gcttcggaat ctgccgggat cacatccggg
    29461 ctctggacga tacgtttctt agcatctttt ttatagatga atgcagtaat acgctggttg
    29521 ccgccctggt tggcatcgat ttgacgaaca atgtagaaag agtaggcacc tttcgctttt
    29581 gctgctttgg tgatggcatc attgacttct ggctggctac gatagaagcc ctggacggtg
    29641 accgtgtcaa acggttcaat cagaacagcc tgatctttcg gcagttcgac aacaccgtta
    29701 attacgcgat tacttgtttc ttctgctttt tcagcatcgg ctttatagag gtcagcgacc
    29761 acacgccagt taccgctgtt accaaaatca gaagtgtcga caacataaaa agaggcggca
    29821 ccttctttat ctgcgcgacg agaaacggct ttcaccgctt cgccaatagc attaaaacga
    29881 ccggtaacca ctacacggtc aaaaggttta accgctgccg cttgctccgg tgtcagttct
    29941 gttgctgcgt taacggagaa tgccgtagca gaaagcagtg ccgacgccag gagggtgttc
    30001 ttaagcttca taaaaataat ccttcgcctt gcgcaaacca ggtactggta ttgttattaa
    30061 cgagaaacgt ggctgattat tgcatttaaa cggtgtaact gtctgcgtca tttttcatat
    30121 cacattcctt aagccaattt taatcctgct caaatgaccg tctatgctta aaaaacagcc
    30181 gtatcagcat cattactact gaagcaactg aattgtataa gttaatttaa tgttaagtag
    30241 tgattcgtgc cggggcgatg tctcgtttta cccgaccgtc gaagacaatt atcagtcttt
    30301 atccggcgtt ctaaggtgtt tatcccacta tcacggctga atcgttaata ttttgcgagt
    30361 tcacgccgaa atactgattt ttggcgctag atcacaggca taattttcag tacgttatag
    30421 ggcgtttgtt actaatttat tttaacggag taacatttag ctcgtacatg agcggcttgt
    30481 gtggctcctg acacaggcaa accatcatca ataaaaccga tggaagggaa tatcatgcga
    30541 attggcatac caagagaacg gttaaccaat gaaacccgtg ttgcagcaac gccaaaaaca
    30601 gtggaacagc tgctgaaact gggttttacc gtcgcggtag agagcggcgc gggtcaactg
    30661 gcaagttttg acgataaagc gtttgtgcaa gcgggcgctg aaattgtaga agggaatagc
    30721 gtctggcagt cagagatcat tctgaaggtc aatgcgccgt tagatgatga aattgcgtta
    30781 ctgaatcctg ggacaacgct ggtgagtttt atctggcctg cgcagaatcc ggaattaatg
    30841 caaaaacttg cggaacgtaa cgtgaccgtg atggcgatgg actctgtgcc gcgtatctca
    30901 cgcgcacaat cgctggacgc actaagctcg atggcgaaca tcgccggtta tcgcgccatt
    30961 gttgaagcgg cacatgaatt tgggcgcttc tttaccgggc aaattactgc ggccgggaaa
    31021 gtgccaccgg caaaagtgat ggtgattggt gcgggtgttg caggtctggc cgccattggc
    31081 gcagcaaaca gtctcggcgc gattgtgcgt gcattcgaca cccgcccgga agtgaaagaa
    31141 caagttcaaa gtatgggcgc ggaattcctc gagctggatt ttaaagagga agcgggcagc
    31201 ggcgatggct atgccaaagt gatgtcggac gcgttcatca aagcggaaat ggaactcttt
    31261 gccgcccagg caaaagaggt cgatatcatt gtcaccaccg cgcttattcc aggcaaacca
    31321 gcgccgaagc taattacccg tgaaatggtt gactccatga aggcgggcag tgtgattgtc
    31381 gacctggcag cccaaaacgg tggcaactgt gaatacaccg tgccgggtga aatcttcact
    31441 acggaaaatg gtgtcaaagt gattggttat accgatcttc cgggccgtct gcctacgcaa
    31501 tcctcacagc tttacggtac taacctcgtt aatctgctga aactgttgtg caaagagaaa
    31561 gacggcaata tcactgttga ttttgatgat gtggtgattc gcggcgtgac cgtgatccgt
    31621 gcgggcgaaa ttacctggcc ggcaccgccg attcaggtat cagctcagcc gcaggcggca
    31681 caaaaagcgg caccggaagt gaaaactgag gaaaaatgtg cctgctcacc gtggcgtaaa
    31741 tacgcattga tggcgctggc aatcatcctt ttcggttggc tggcaagcgt tgcgccgaaa
    31801 gagtttcttg ggcacttcac cgttttcgcg ctggcctgcg ttgtcggtta ttacgtggtg
    31861 tggaatgtat cgcatgcgct gcatacaccg ttgatgtcgg tcaccaacgc gatttcaggg
    31921 attattgttg tcggagcact gttgcagatt ggccagggcg gctgggttag cttccttagt
    31981 tttatcgcgg tgcttatagc cagcattaat attttcggtg gcttcaccgt gactcagcgc
    32041 atgctgaaaa tgttccgcaa aaattaaggg gtaacatatg tctggaggat tagttacagc
    32101 tgcatacatt gttgccgcga tcctgtttat cttcagtctg gccggtcttt cgaaacatga
    32161 aacgtctcgc cagggtaaca acttcggtat cgccgggatg gcgattgcgt taatcgcaac
    32221 catttttgga ccggatacgg gtaatgttgg ctggatcttg ctggcgatgg tcattggtgg
    32281 ggcaattggt atccgtctgg cgaagaaagt tgaaatgacc gaaatgccag aactggtggc
    32341 gatcctgcat agcttcgtgg gtctggcggc agtgctggtt ggctttaaca gttatctgca
    32401 tcatgacgcg ggaatggcac cgattctggt caatattcac ctgacggaag tgttcctcgg
    32461 tatcttcatc ggggcggtaa cgttcacggg ttcggtggtg gcgtttggca aactgtgtgg
    32521 caagatttcg tctaaaccgt tgatgctgcc aaaccgtcac aaaatgaacc tggcggctct
    32581 ggtcgtttcc ttcctgctgc tgattgtatt tgttcgcacg gatagcgtcg gcctgcaagt
    32641 gctggcattg ctgataatga ccgccattgc gctggtattc ggctggcatt tagtcgcctc
    32701 catcggtggt gcagatatgc cagtggtggt gtcgatgctg aactcgtact ccggctgggc
    32761 ggctgcggct gcgggcttta tgctcagcaa cgacctgctg attgtgaccg gtgcgctggt
    32821 cggttcttcg ggcgctatcc tttcttacat tatgtgtaag gcgatgaacc gttcctttat
    32881 cagcgttatt gcgggtggtt tcggcaccga cggctcttct actggcgatg atcaggaagt
    32941 gggcgagcac cgcgaaatca ccgcagaaga gacagcggaa ctgctgaaaa actcccattc
    33001 agtgatcatt actccggggt acggcatggc agtcgcgcag gcgcaatatc ctgtcgctga
    33061 aattaccgag aaactgcgcg ctcgtggtat caacgtgcgt ttcggtatcc acccggttgc
    33121 ggggcgtttg cctggacata tgaacgtatt gctggctgaa gcaaaagtac cgtatgacat
    33181 cgtgctggaa atggacgaga tcaacgatga ctttgctgat accgataccg tactggtgat
    33241 tggtgctaac gatacggtta acccggcggc gcaggatgat ccgaagagtc ctattgctgg
    33301 tatgcctgtg ctggaagtgt ggaaagcgca gaacgtgatt gtctttaaac gttcgatgaa
    33361 cactggctat gctggtgtgc aaaacccgct gttcttcaag gaaaacaccc acatgctgtt
    33421 tggtgacgcc aaagccagcg tggatgcaat cctgaaagct ctgtaaccct gacggcctct
    33481 gcggaggccg tcactcttta ttgagatcgc ttaacagaac ggcgatgctt tgacctcccg
    33541 ctgtttgttc aagcgcaatt ttgacaataa ttgttaacgg cacggaaagc agcataccca
    33601 ccggtcctaa caaccatccc caaaaaatca acgacaaaaa taccaccaat gtggaaagcc
    33661 ccagcccacg tcccatgatg cgcggctcca gaatattgcc gaagaccaga ttaatcagca
    33721 gatatcccgc cagcaccagc aacgcttcgt agaagccatt aaacaccagt acctgagcga
    33781 taggggggat tgccgcgaga actgaaccaa tattagggat gtaattaagc gcaaaggcca
    33841 gcaatcccca gacaaaagcg aagcgaacat cgagtgcggc gagcatcgcc caggcgacca
    33901 ggccggtgat gatgctgatg gctgttttca gcaccagata atgagaaaca ctgtcaattg
    33961 cacgttgaat cgccgccatc ccttcaaccg gacgcgccat catttgctga aattttccgg
    34021 gcaattgtgg cacttcgagc agcataaaca gcaccgtcag cagcaataaa aatattgatg
    34081 acatggcatt agataactgc gtcaataagt tggtgagcaa cgtcatcgcc gcgttcggat
    34141 caatgtaatg cgccagctgg tcaactgaga cgtcaatccc tacgcgttgc aacaacggct
    34201 caagagcttg cagcggcgtc ataatagagt tgcgatattg cggtaacgtc cgcgtcaact
    34261 cgttgagcgc ggaacccaga taagctaata gcaacaccat cgccatcacg atgatggtca
    34321 tcaaaatcga caccgccagt acacgcggca ctcgccagcg gaccatgtgt tgcaccagcg
    34381 ggttaagaat aacagcaata aataatgcga gaataaacgg cacgatgatc tcggcggcaa
    34441 aacggatacc gcagagaata atgaccagca ttcccaacat aatgacgatt tttaggccat
    34501 tgagcgtgat gatcggcttt gccatgcttg tccccgtttt ttcttccttt gctaataata
    34561 atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa gaagtgaaaa
    34621 tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta ccgaggacaa
    34681 ttatcatccg cgatgacgag aagcaacact gcggataatt gtaatattat ggacaatttg
    34741 ttcaggacgt tattttcaac atttacccat cttcgtacct catccactat cttgctcgta
    34801 ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct tgccctatgc
    34861 gcggggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa aataattctc
    34921 ttgcaggaga aggacaatgt atatttattg gattttatta ggcctggcta ttgctacaga
    34981 aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg gcggctttat
    35041 tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg ttaaaaagat
    35101 cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta ttaccttgtt
    35161 tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt taaccaccct
    35221 ggtcgccggg attgtgttga tcaaatcagg tacccgtaaa gcgcgtaaac ctgaactgga
    35281 ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg gcaatcgtgc
    35341 tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc aaaatatttg
    35401 gcttgctctc cctggcggcg gtgctggctg cctttagtgc gctttctcag gccgtaaaag
    35461 ggatcgactt gtctgtcgct tatgcattgt ggggcgggtt tggtattgcc gccacgttag
    35521 ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt ggcctggtct
    35581 tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc ccgcgctgtc
    35641 gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga aaaccggtaa
    35701 cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc gaactttgca
    35761 ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg ctgtcacccg
    35821 gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc acttcacagt
    35881 tttgatgact gtataacgta tcgagatgat cttcaggata tcccgcctga gtcactttac
    35941 gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat aacggcaacg
    36001 gtgtaatgcc agaaggggga ttacgcagca caatcaagcc gaagtcccat ggtgcagctg
    36061 cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt gtcggatcaa
    36121 cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac acaaaacgca
    36181 gcgccactgc tttatcggct ttaccctttg gaggtgtcaa taaacagtgt cctgccgtta
    36241 atgccagatt gggtgcaatc agcgtcgccg tacacaaatt gccgctggcc gtttccagtt
    36301 gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga tcatcatgac
    36361 caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt ttatctgcaa
    36421 acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt gtacgcatat
    36481 cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac tatagacggg
    36541 acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa aaacgggcgg
    36601 caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa cggcccacca
    36661 tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta attttcaggc
    36721 gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg cagcaggttt
    36781 tgccggttgc tgatggctgt gtttcccggc gtgtttcttc gctgcctgcg ctttttgttc
    36841 aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct gcgctttctg
    36901 ggcaggtgct gctttatgct gttttttatg atgtgtcgtt ttcgccggcg ctgctttggt
    36961 ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg cagaagacag
    37021 acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac cctcaatttg
    37081 ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg gaatggcaga
    37141 cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa taaattacag
    37201 cgttgataat aacgtattgt gacttaaggg aaatttagct accaataata gtagtcttga
    37261 tcgggttaac tattttacta cttacaaagc ggtagaataa ctgcgcatca gtaataaatg
    37321 acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac gctagacgcg
    37381 ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt ggtttgagca
    37441 agcaataagt ccggacgtgt agttaccgca gtccgggctt atttttcagg cgtgcagacg
    37501 acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa taaatgcgcc
    37561 gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg caatactcga
    37621 ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc gttttgcgcg
    37681 ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg agaagagtaa
    37741 cattccggca aagatcagcc acagcgagtt gaataaggtc atcagtaaac caaacagcat
    37801 aacccccgtc gaaaacaaca tcaccggacc gcgcccgtag cgggtggtca tggttccggc
    37861 tttaggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa ccacggcctg
    37921 actgacatgc cagggggaaa gcatcaaccg atagccgatg taattaaaca gcgtgacgaa
    37981 cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt cacgccagtg
    38041 cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcgaa aatggcgtga
    38101 ttccggaagg attttccaga acatcaacgc cgaggctagc gcgaaacaac cgattgccgc
    38161 cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc gtccgctcat
    38221 gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga aactggggtg
    38281 gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaatg aaagcccaat
    38341 caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa gtaacgtaca
    38401 aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg catcggatag
    38461 ggggccagta aacagcaaac caatagccaa catcgccgtg gaaatggaca gtgaaatact
    38521 actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag gttgcacaca
    38581 atagagaagt gcgaaggttg ccagtccggc agagaacagc gccagggtga cgcgcataaa
    38641 ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct tgtcagtgtc
    38701 gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt gctaaatatg
    38761 cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct aatatattaa
    38821 taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc gttactttgt
    38881 tgctgttgcg gaagagctgc atttcgggcg cgccgctgcc cgcctgaata tttcgcaacc
    38941 accgctaagt cagcagattc aggcactgga gcaacaaatt ggtgcccgac tgctggcacg
    39001 aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca
    39061 aatcctgtct atggtagatg acgctgccgc tcgcgctgaa agactgcatc agggggaagc
    39121 gggggagttg cgcattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac
    39181 gttatcgctg tttcgccgtg attatcccga tgtccattta caaacccgcg aaatgaacac
    39241 tcgtgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac
    39301 agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat
    39361 cccgcacgat catcccctgg caaataaccc gaatgtaacg ctggctgaac tggcgaaaga
    39421 accctttgtc ttttttgatc cgcacgtcgg gacagggctg tatgacgata ttctcggact
    39481 gatgcgacgt taccatttga cgcccgtcat cactcaggag gtgggcgagg caatgaccat
    39541 catcggtctg gtttccgccg gtctgggtgt ttcaattttg cctgcttcat ttaaacgtgt
    39601 tcagctcaac gaaatgcgct gggtgccgat tgctgaagag gatgcggttt ctgaaatgtg
    39661 gttggtctgg ccgaaacatc atgaacaaag tccagctgcg cgtaactttc gtattcatct
    39721 gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc ccgaaaaatg tgctgttaat
    39781 cacatgccta agtaaaaatt tgacgacacg tattgaagtg cttcaccata gcctacagat
    39841 tatttcggag cgcgaaaata tagggagtat gcggtggttg ctgaaaacca gcctgggcac
    39901 attgatcaaa taaagcagac caacgcgggc gcggtttatc gcctgattga tcagcttggt
    39961 ccagtctcgc gtatcgatct ttcccgtctg gcgcaactgg ctcctgccag tatcactaaa
    40021 attgtccgtg agatgctcga agcacacctg gtgcaagagc tggaaatcaa agaagcgggg
    40081 aaccgtggcc gtccggcggt ggggctggtg gttgaaactg aagcctggca ctatctttct
    40141 ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg atctgagcag caaactggtg
    40201 gtggaagagt cgcaggaact ggcgttaaaa gatgactcgc cattgctgga tcgtatcatt
    40261 tcccatatcg atcagttttt tatccgccac cagaaaaaac ttgagcgtct aacttcgatt
    40321 gccataaccc tgccgggaat tattgatacg gaaaatggca ttgtacatcg catgccgttc
    40381 tacgaggatg taaaagagat gccgctcggc gaggctctgg agcagcatac cggcgttccg
    40441 gtttatattc agcatgatat cagcgcatgg acgatggcag aggccttgtt tggtgcctca
    40501 cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca acgtgggggc gggcgtcatt
    40561 accgatggtc atctgctaca cgcaggcagc agtagtctcg tggaaatagg ccacacacag
    40621 gtcgacccgt atgggaaacg ctgttattgc gggaatcacg gctgcctcga aaccatcgcc
    40681 agtgtggaca gtattcttga gctggcacaa ctgcgtctta atcaatccat gagctcgatg
    40741 ttacatggac aaccgttaac cgtggactca ttgtgtcagg cggcattgcg cggcgatctg
    40801 ctggcaaaag acatcattac cggggtgggc gcgcatgtag ggcgcattct tgccatcatg
    40861 gtgaatttat ttaacccaca aaaaatactg attggctcac cgttaagtaa agcggcagat
    40921 atcctcttcc cggtcatctc agacagcatc cgtcagcagg cccttcctgc gtatagtcag
    40981 cacatcagcg ttgagagtac tcagttttct aaccagggca cgatggcagg ggctgcactg
    41041 gtaaaagacg cgatgtataa cggttctttg ttgattcgtc tgttgcaggg ttaacatttt
    41101 ttaactgttc taccaaaatt tgcgctatct caatttgggc caggaaagca taacttagac
    41161 tttcaaggtt aattattttc ctggtttata tttgtgaagc ataacggtgg agttagtgat
    41221 gctgaagcgt ttctttatta ccggtacaga cacttctgta gggaaaacgg tggtttcccg
    41281 cgcattgcta caagcgttag cctcccaggg aaaaacggtt gcgggatata aacccgtagc
    41341 gaaggggagc aaagagacac ccgaagggct gcgtaataaa gatgccctgg tgttgcagag
    41401 tgtttcaacc atcgaactgc cttatgaagc agttaatcct atcgcgttaa gcgaagaaga
    41461 aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc atttcaaacg gcctggcaaa
    41521 cctgaccgaa aaagtcgatc atgtcgtggt agaagggact ggcggctggc gcagtctgat
    41581 gaatgatttg cgtccactct ctgaatgggt agtgcaggaa caactgccgg tgttgatggt
    41641 tgtcggtatt caggaaggtt gcattaacca tgcactgcta acagctcagg cgatcgccaa
    41701 cgacgggctg ccgctcattg gctgggtggc taaccgaatc aacccaggac tggcgcatta
    41761 tgcggaaatc attgatgtgc tgggtaaaaa acttccggca ccgctcattg gtgaactgcc
    41821 ttatctgccg cgcgccgaac agcgtgaact ggggcaatac atccgcttag ctatgctgcg
    41881 cagtgtgctg gcggtagata gagtcacggt gtaacgtccg cgaaattacc gacgcaatta
    41941 cgcaggcaat caataaaccg gggagtagct gatactcccc ggtcatttca catatcatca
    42001 acgtcgacat aatcggcgcg tgcgtggtcg ccgccaacag tgtcgccatc ccggtcaatc
    42061 cgagtaaaag tgtaatttct tcgccatcag ggaaccataa tcccaggcta cgaccataca
    42121 acatgccaat ggcaagaccg ataaatagcg tcggtgtaaa gacaccaccg ggtgcgccgg
    42181 aaccgctact cgccagcacg gcacacagtt tacaaaggaa gatcccggca atgatcatta
    42241 acagtggtgg ggcggttaaa aaggattgta cggtgctata gccgttgccc cacactgcag
    42301 gtgtgaacag ggaaagcaga cccacgatca acccgcctag tgccagttgc cagggcggcg
    42361 caagtttgag actcacaaat ccacgatgac aggcgttcat taacgttaac aacagtggtc
    42421 cgcacagacc tgccagcaca cctgtactga taatcagcgc atagtcacga gcctgaaccg
    42481 tcactgagag ttgtacgttg tagagtaacg cgtcgctatg attaatcaga ttgctaacca
    42541 gcaatgccac gacggcggaa ataatcaccg ggccgagaga ggccaacatc atagtgccaa
    42601 acagcacttc ggctataaat aaactgccag caagcggggc acgataggcc gcagccattc
    42661 ccgccgcggc cccacaggcg atccataatt tccactcctg gcgtggcgta aaacgttggg
    42721 caaaacagga ggcggcaagg gcagctaaaa gaatcatcgc accttcgcga ccaattgcac
    42781 tgccgctggt tactaccagc agagaggcaa gcgatttaac caggcttgct gcgtaatcga
    42841 actgtccatc ggtttgcaac gcttccatgt aatcggtcgg cgcatgaggg cgttgttggg
    42901 taaatttctg ccagcccatc agcaacaaac ccgccgccag tccgccgagc gccggagtta
    42961 gcaaccgtcg ccagggggaa aggtttgtcg ctgcattgac cagactgccg gagtcattat
    43021 tgaggaacaa ccactccagt agcagcatcg catgacgaaa cccggcaacg gcaaaggccg
    43081 cgagaatacc gacgactgtt gcgataagca gacggcggaa catagtgcgc agatccggat
    43141 agatgtgtag atgatgcatg agcagaaatg ggcaatgaaa cacgggcgac tattttgcgg
    43201 caaaagaggc cagccgcaaa agcagtgcgt gctttaagag ataaaaaaaa cgtgacactg
    43261 taacctatta ttgccggatt gcgcgtaatc gtcaccatcc ggcaatatta cggtgatcct
    43321 atcgaaacag cggtttaacc gcgacaggaa ttaacagttg tgattgccat tgcgccagcg
    43381 ttaagcgagc cagttcaccc agtgcacggt agaaagggtg ttctgctttt tcgataaaaa
    43441 catcaagaaa acgtgttgac cacggaaaaa ggtgccatgc cagcaattct tcgcattctg
    43501 tctggcgacc attttccgcc aaccacgcag ccatcaacag cagtgaacca aaatgatctt
    43561 ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc gcgcatccac tgacgaagtg
    43621 ccaatgttga atcgccaaac agcacagatt cgcgatccag ccagaccgac ccccacggcg
    43681 gagacggcag tgcccacggg ccgacaaaca aacgctgcca ggcctgggcg tgagtctctt
    43741 cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga cgcttctggt agaggccact
    43801 gagtttccca gccgtcactg gtgagtaccg caaccagagg tgcggcttcc gcactctctg
    43861 gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac agaaaaatta tcttgctgtg
    43921 aaaaatgggt cattcctaac atcctgaaat gtgcgggttg ccccgcacca atttgttaac
    43981 ctgcaatagc cattcctact gtcatgtgca ggccataaaa gagcacacgc ccgatcatct
    44041 caccgccaag tatgaggatt agccccagaa taagtccggc aacgtgaggt tcacggcgac
    44101 gaatgagcgg gcaaagccag catccaagac ctgcgcataa caataccaca cgccagacct
    44161 gtaaggaggc gtaatctggt accagtgcgc tggcttgttg cacggatgag tgaatagacg
    44221 ccagagacaa accttgcagc acaatcaccc cggcacaagc gatcagcgcc agaacgctaa
    44281 tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc ccgcagaatt gcggcagcaa
    44341 gtattggacc gctcaacaat accgtcagga agaaagccag cgtagtgtaa ccgttatgcc
    44401 aggttggcac agtgtcgatt tgatacacgc aggtcatcat ccagacgaaa atgacgccga
    44461 gcgccatgct gacaagtaac cagagtttcc ccaacgcttg cggcatttta ccgatgaccg
    44521 ccaccagcca ccatagtccg ccaacggcaa aaaatatgga accggcagca atctcgttac
    44581 tcagaccaga agctccgatt cgattcagcg agttaaacgc ccgcagtggc gatcccaggt
    44641 gcatgacaga ggcaataaac ccgatgccca tcaacagcca gaggaaaaac atgccacgga
    44701 caatacgctg acggtctgcg tcatttttgg cggcgaacca gccaattccg ctaacaatca
    44761 gcgcgcctac tacgcattga cccaatacgg taaagataac cagtggccac tcatgccatc
    44821 catttcccat tttacacttc ctccggatta gccagataac cagtggtatc gccggtcggg
    44881 cggctgttgg cgttaggttt gataacgata ttgggttttg tgaaatgcgc gcgcggcagt
    44941 ggcgcgacgg cagccagagt gccgtgcttc tggcgcagct cttcaattgg accgaactca
    45001 agtgcacgca gagggcagga ttcgacacat atgggttgtt tcccctcggc gacgcgcgaa
    45061 taacaaccat cgcacttcgt catgtgccct ttttcagcat tgtactgtgg cgcgccgtac
    45121 ggacaagcca tgtggcagta gcggcagccg atacaaacat cttcatcaac caccacaaag
    45181 ccatcttcac gcttatgcat tgcgccgctg gggcagactt ttgtacatgc ggggtcatcg
    45241 caatggttac aggagatgga gagataataa gcgaacacgt tctggtgcca gacgccatta
    45301 tcttcctgcc agtcgccgcc agcgtattca taaatacggc ggaaactgac ttccgggcca
    45361 agatctttaa aatctttgca cgccagttcg caggttttac agccagtaca gcggctggaa
    45421 tcgataaaaa atccatattg tgtggtcatg ggctactcct taaacctttt cgatctggac
    45481 aagattgctg tgcgacgggt ttccctttgc cagcggtgaa gggcggtgag aggtcagaat
    45541 attgatactg ccgccgtgat cgacccggtc accaaacata tccgctttaa gccacgcacc
    45601 ttgcccgatg gcggtaacgc caggcagaat acgcggagtc acttttgcgg caatcagcat
    45661 ttctccatta ttgttaaata cccgcacggt atcgccatga cggataccgc gtgcctgagc
    45721 atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc tgtaacacat caatattgcc
    45781 gtagctggag tgggtacgcg ctttgtagtg gaagcccgtt aactgcagtg gataggtttt
    45841 ccgcagggga tcgtcccagc catcaaaacc tggggtatac gcaggaaggg gatgaataat
    45901 ttcatctttt ttcaattccc aggtatctgc aatcttcgcc agtcgttccg aataaatttc
    45961 gattttcccc gaaggtgttt tcaacgggtt tgcctgtgga tcttcacgga atgcgcggaa
    46021 agcgacgtag tgttcttccg ggcatttttt cttaaagatc ccggtcgttt tcatctcctc
    46081 gtagtcgggc atctcagggt tacgttcctt cgttttcgca tggagatatt tgatccattc
    46141 atgctgactg cgaccttcag taaaggtttg ataaacgtct ggtcctaagc gtttggcgac
    46201 ttcactcagc atccagtaga tgggtttgcg ttcaaatttt gctgaggttg cgggttgggc
    46261 gaggatcaca tagcccatat tccctgcaga ttcatgagag ataaggtctt cttgctctgt
    46321 tggcatcagg tcgggcaaca ggatatcgca atacttagcc gaggccgtca tgaagtggtc
    46381 aatgccaaca atcatctcgc acttgctgtc atcctgaagc acctcatggg tgtgattgat
    46441 gtcgccatgt tgattgatca atgtgttact ggcgtagcac cataaaaact tgatggggac
    46501 atccagtttt tcttttccac gaacaccatc acgggtcgcg gtcatttccg taccatggtc
    46561 gatggcatct gtccatgtaa agacggaaat ctgcgtttta acaggattct caagcatcgg
    46621 gaaccattct acccccagat cccagctacc ttcgcgtaca cctgagttgc cgccgtttat
    46681 gccgacgtta ccggtgagaa cggaaagcat ggcaatagcg cgggacgttt gctcgccgtt
    46741 ggaatgtcgt tgtggccccc aaccctgaca aatataagca ggttttgctg aaccgatctc
    46801 tcgtgccaac tggataattt tttctgccgg gatgctggtg atttttgctg cccattccgg
    46861 cgttttagct atgccgtcag gcccttcgcc cagaatatag gctttataat gcgcgttacg
    46921 tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca caatatttgt cgagaaatgg
    46981 ctgatcgacc atgttttcag taatcagtac ccaggcaatc gcacaggcca gtgcgccatc
    47041 ggtgccaggg cgaatgggca gccattcatc ttcacgcccg gcagcagtgt cgttataacg
    47101 tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg gcttgctcga cgtagtaagt
    47161 gacaccaccg ccgctcatcc gcgtttctgc cgggttattt ccgaacataa cgaccagttt
    47221 cgtattggcg atatcatccg ggctgttgcc atcattggca ccgaacatat aactcattgc
    47281 ggcactgatc tgtgcggtac tgtagctgcc atagcgactg agaaaaccac cgcaagagtt
    47341 catcagacgg tacgggacgt ttgagttggt gatgtttccg ccatctacgc ctgttccgta
    47401 caggacatgt acagcctcat tgccgtaatc tttcaggatc cgccgaagat tatcactgat
    47461 ggtatccagg gcttcgtccc aacttatccg ttcaaattta ccttcaccgc gcttgccgac
    47521 gcgcttcatg ggatatttca acctatcagg atgattcatc cgtcggcgga tagagcgccc
    47581 gcgtaaacac gctcgaacct gatggttacc gtagacgtcg tcacctgtcg tatcagactc
    47641 cacccagtac acggtgtcat ctttcacatg caaacgtaac agacagcggc tcccgcagtt
    47701 aacggtgcag gaactccaga ccgctttctc ttctaccgga gcctctgccg cccggaccat
    47761 ttgggaaaat ggcagagtga aagcactgct tgccagcgca agactgccaa gtgcggaggt
    47821 tttcatcaga cttctacggc tgatttcagc cttcatgagc gcctctgtgg tatggatttt
    47881 catcattact cacttattgc ttttcaaaca aaatgtcatg ccagaattta tggttgtcgt
    47941 gggttatatt ttt
//
LOCUS       DAPHDX010000017        46732 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPHDX010000017 DAPHDX010000000
VERSION     DAPHDX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.017"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(286..828)
                     /locus_tag="RH058_000937"
     CDS             complement(286..828)
                     /locus_tag="RH058_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3136562.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(851..1876)
                     /gene="nagZ"
                     /locus_tag="RH058_000938"
     CDS             complement(851..1876)
                     /gene="nagZ"
                     /locus_tag="RH058_000938"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV3136563.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1887..2711)
                     /gene="thiK"
                     /locus_tag="RH058_000939"
     CDS             complement(1887..2711)
                     /gene="thiK"
                     /locus_tag="RH058_000939"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV3136564.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2692..3333)
                     /gene="lpoB"
                     /locus_tag="RH058_000940"
     CDS             complement(2692..3333)
                     /gene="lpoB"
                     /locus_tag="RH058_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV3136565.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3347..3724)
                     /locus_tag="RH058_000941"
     CDS             complement(3347..3724)
                     /locus_tag="RH058_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV3136566.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3727..4086)
                     /gene="hinT"
                     /locus_tag="RH058_000942"
     CDS             complement(3727..4086)
                     /gene="hinT"
                     /locus_tag="RH058_000942"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV3136567.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4420..6609
                     /gene="fhuE"
                     /locus_tag="RH058_000943"
     CDS             4420..6609
                     /gene="fhuE"
                     /locus_tag="RH058_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV3136568.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTQGISKSQADSDRALYYTRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWTNIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6669..8102)
                     /gene="ptsG"
                     /locus_tag="RH058_000944"
     CDS             complement(6669..8102)
                     /gene="ptsG"
                     /locus_tag="RH058_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV3136569.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8397..9194)
                     /locus_tag="RH058_000945"
     CDS             complement(8397..9194)
                     /locus_tag="RH058_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3136570.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9205..10209)
                     /gene="holB"
                     /locus_tag="RH058_000946"
     CDS             complement(9205..10209)
                     /gene="holB"
                     /locus_tag="RH058_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV3136571.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10206..10847)
                     /locus_tag="RH058_000947"
     CDS             complement(10206..10847)
                     /locus_tag="RH058_000947"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV3136572.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10837..11859)
                     /gene="yceG"
                     /locus_tag="RH058_000948"
     CDS             complement(10837..11859)
                     /gene="yceG"
                     /locus_tag="RH058_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV3136573.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11862..12671)
                     /gene="pabC"
                     /locus_tag="RH058_000949"
     CDS             complement(11862..12671)
                     /gene="pabC"
                     /locus_tag="RH058_000949"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV3136574.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12791..14032)
                     /gene="fabF"
                     /locus_tag="RH058_000950"
     CDS             complement(12791..14032)
                     /gene="fabF"
                     /locus_tag="RH058_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV3136575.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14120..14356)
                     /gene="acpP"
                     /locus_tag="RH058_000951"
     CDS             complement(14120..14356)
                     /gene="acpP"
                     /locus_tag="RH058_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3136576.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14567..15301)
                     /gene="fabG"
                     /locus_tag="RH058_000952"
     CDS             complement(14567..15301)
                     /gene="fabG"
                     /locus_tag="RH058_000952"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV3136577.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15314..16243)
                     /gene="fabD"
                     /locus_tag="RH058_000953"
     CDS             complement(15314..16243)
                     /gene="fabD"
                     /locus_tag="RH058_000953"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV3136578.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16259..17212)
                     /gene="fabH"
                     /locus_tag="RH058_000954"
     CDS             complement(16259..17212)
                     /gene="fabH"
                     /locus_tag="RH058_000954"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV3136579.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17280..18350)
                     /gene="plsX"
                     /locus_tag="RH058_000955"
     CDS             complement(17280..18350)
                     /gene="plsX"
                     /locus_tag="RH058_000955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV3136580.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18431..18604)
                     /gene="rpmF"
                     /locus_tag="RH058_000956"
     CDS             complement(18431..18604)
                     /gene="rpmF"
                     /locus_tag="RH058_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV3136581.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18656..19177)
                     /gene="yceD"
                     /locus_tag="RH058_000957"
     CDS             complement(18656..19177)
                     /gene="yceD"
                     /locus_tag="RH058_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV3136582.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19376..19960
                     /gene="yceF"
                     /locus_tag="RH058_000958"
     CDS             19376..19960
                     /gene="yceF"
                     /locus_tag="RH058_000958"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV3136583.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20072..21031)
                     /gene="rluC"
                     /locus_tag="RH058_000959"
     CDS             complement(20072..21031)
                     /gene="rluC"
                     /locus_tag="RH058_000959"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV3136584.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21604..24789
                     /gene="rne"
                     /locus_tag="RH058_000960"
     CDS             21604..24789
                     /gene="rne"
                     /locus_tag="RH058_000960"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV3136585.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24986..25939)
                     /gene="flgL"
                     /locus_tag="RH058_000961"
     CDS             complement(24986..25939)
                     /gene="flgL"
                     /locus_tag="RH058_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV3136586.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25951..27594)
                     /gene="flgK"
                     /locus_tag="RH058_000962"
     CDS             complement(25951..27594)
                     /gene="flgK"
                     /locus_tag="RH058_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV3136587.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVNVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     ILQNTKNKGDVAIGATVTDASGVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27660..28601)
                     /gene="flgJ"
                     /locus_tag="RH058_000963"
     CDS             complement(27660..28601)
                     /gene="flgJ"
                     /locus_tag="RH058_000963"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV3136588.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28601..29698)
                     /gene="flgI"
                     /locus_tag="RH058_000964"
     CDS             complement(28601..29698)
                     /gene="flgI"
                     /locus_tag="RH058_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV3136589.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29710..30408)
                     /gene="flgH"
                     /locus_tag="RH058_000965"
     CDS             complement(29710..30408)
                     /gene="flgH"
                     /locus_tag="RH058_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV3136590.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSYANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFNGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30461..31243)
                     /gene="flgG"
                     /locus_tag="RH058_000966"
     CDS             complement(30461..31243)
                     /gene="flgG"
                     /locus_tag="RH058_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV3136591.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31381..32136)
                     /gene="flgF"
                     /locus_tag="RH058_000967"
     CDS             complement(31381..32136)
                     /gene="flgF"
                     /locus_tag="RH058_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV3136592.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32156..33361)
                     /gene="flgE"
                     /locus_tag="RH058_000968"
     CDS             complement(32156..33361)
                     /gene="flgE"
                     /locus_tag="RH058_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV3136593.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33386..34081)
                     /gene="flgD"
                     /locus_tag="RH058_000969"
     CDS             complement(33386..34081)
                     /gene="flgD"
                     /locus_tag="RH058_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV3136594.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34093..34497)
                     /gene="flgC"
                     /locus_tag="RH058_000970"
     CDS             complement(34093..34497)
                     /gene="flgC"
                     /locus_tag="RH058_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV3136595.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34501..34917)
                     /gene="flgB"
                     /locus_tag="RH058_000971"
     CDS             complement(34501..34917)
                     /gene="flgB"
                     /locus_tag="RH058_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV3136596.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35072..35731
                     /gene="flgA"
                     /locus_tag="RH058_000972"
     CDS             35072..35731
                     /gene="flgA"
                     /locus_tag="RH058_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV3136597.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35807..36100
                     /gene="flgM"
                     /locus_tag="RH058_000973"
     CDS             35807..36100
                     /gene="flgM"
                     /locus_tag="RH058_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV3136598.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36105..36521
                     /gene="flgN"
                     /locus_tag="RH058_000974"
     CDS             36105..36521
                     /gene="flgN"
                     /locus_tag="RH058_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV3136599.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36561..38096)
                     /gene="murJ"
                     /locus_tag="RH058_000975"
     CDS             complement(36561..38096)
                     /gene="murJ"
                     /locus_tag="RH058_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV3136600.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38206..39129)
                     /locus_tag="RH058_000976"
     CDS             complement(38206..39129)
                     /locus_tag="RH058_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3136601.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39131..39778)
                     /locus_tag="RH058_000977"
     CDS             complement(39131..39778)
                     /locus_tag="RH058_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV3136602.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLVHLGD"
     gene            complement(39789..40373)
                     /gene="rimJ"
                     /locus_tag="RH058_000978"
     CDS             complement(39789..40373)
                     /gene="rimJ"
                     /locus_tag="RH058_000978"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV3136603.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40609..41817
                     /gene="mdtH"
                     /locus_tag="RH058_000979"
     CDS             40609..41817
                     /gene="mdtH"
                     /locus_tag="RH058_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV3136604.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41881..42528
                     /gene="grxB"
                     /locus_tag="RH058_000980"
     CDS             41881..42528
                     /gene="grxB"
                     /locus_tag="RH058_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV3136605.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42662..43222
                     /locus_tag="RH058_000981"
     CDS             42662..43222
                     /locus_tag="RH058_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3136606.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43328..44374
                     /gene="pyrC"
                     /locus_tag="RH058_000982"
     CDS             43328..44374
                     /gene="pyrC"
                     /locus_tag="RH058_000982"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV3136607.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44448..44693
                     /gene="dinI"
                     /locus_tag="RH058_000983"
     CDS             44448..44693
                     /gene="dinI"
                     /locus_tag="RH058_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV3136608.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44983..45237
                     /gene="bssS"
                     /locus_tag="RH058_000984"
     CDS             44983..45237
                     /gene="bssS"
                     /locus_tag="RH058_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV3136609.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45352..46470
                     /gene="solA"
                     /locus_tag="RH058_000985"
     CDS             45352..46470
                     /gene="solA"
                     /locus_tag="RH058_000985"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV3136610.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46518..46631
                     /locus_tag="RH058_000986"
     CDS             46518..46631
                     /locus_tag="RH058_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV3136611.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        11338 a        12580 c        11603 g        11211 t
ORIGIN      
        1 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg
       61 ggtgagatca aaaaaataga gtttattgtt tattatttga gcaaattcgg aagagtaata
      121 tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc
      181 aggcattgat gctcgactgt cggatgcggc gtggacgcct tatccgactt acagaattcg
      241 gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt
      301 gaacgcttta atgcgctgta aatgcgggga gatattcttg aatttgtgcg tctgctcttc
      361 gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagtgc
      421 ttcatcatta cgcgacaaaa taaccagaca acgatcgcga ttcttctcgc ggaagttggt
      481 cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt
      541 ctcataaggg aacaaattag ggttgaagat cacctggcgg atgtcgcaaa gaaaaccaat
      601 ccgttccgcc cagtatccgc ccaggccaac gccgcaaatt agcggacgct cgtcaacgtt
      661 cagttgcaac attttgtcca cttctttaag cagatgctgc atatcatgtt tcggatgccg
      721 cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc
      781 gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca
      841 taagccaggg ttagtgacct gctttctctt cctgccagcg ttcgtgtaac tgattcagac
      901 gggtgctgat cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt
      961 tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga
     1021 cggccccttt acgattattg cagaccagga tcatatcgca acccgcatcc agtgatgcct
     1081 gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga
     1141 aaatcacgcc gtcaaaaccc agttcctgac gcaaaacggt tttcagccag tagggagaac
     1201 cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga
     1261 gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt
     1321 gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg
     1381 ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg
     1441 caattgccag ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga
     1501 tatgcccgac atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc
     1561 tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cctgacagcg
     1621 cagcgaatga ttgcgccgct ggcaagcggg taaaaccttc acgaaaacgc tgcacgcgtc
     1681 caccttcctg atctaccgcc accaccagat gattgcgcga agctgcgcgg atctggcgca
     1741 ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca
     1801 ccagcggatg cgcaagtatt tcacgctctt ccgcgtccag ttcgtaacct ttgacatcca
     1861 acattactgg acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca
     1921 tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct
     1981 gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga
     2041 taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc
     2101 acccacaccg ccgccagttc cagcgcgata tcaccatctc cggcatactc ccagtcgatg
     2161 agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga
     2221 cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa
     2281 cccactgtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa cggcaacagc
     2341 gttattcgcc agccaaaacg tggttgttga tgtagataat acagcaagcc tgccagttcg
     2401 ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca
     2461 cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac
     2521 tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc
     2581 tggccctgat gttcaatgag aaaactcccg ccactaagcc cactattaaa cgtcgtgacg
     2641 ggatgaaact gcgggaaaaa gcgcgacagt aattcgtcgc gcgtgatggg attattgctg
     2701 cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat
     2761 ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc
     2821 gacattgcgg gcaatgccta tggctttact acgggtgcct aaactgtcct gcggcgacaa
     2881 acctaactgt tgcttagcca tcgacagctg ctgggcggaa accagggtaa atttcccgtt
     2941 attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt
     3001 acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag
     3061 catcttactg accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc
     3121 aggcggtgcc gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac
     3181 cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac
     3241 ttcttctacc ggtgcaggtt cacgttgccc cacacatccg gcgagaaaca tcgccagcgc
     3301 ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat
     3361 aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat
     3421 gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt
     3481 cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg
     3541 aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg
     3601 cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat
     3661 gtgaacgaca gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc
     3721 tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg
     3781 catatggatg tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata
     3841 gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc
     3901 cagcgcctgc tcatgctcag ctgagacgtc gttcacagtc ggaatgagga tattcggaat
     3961 gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc
     4021 ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc
     4081 tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca
     4141 ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct
     4201 taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa
     4261 tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg tatatttctc
     4321 atttgcattt acaaacaaaa ttattcgcac actaaatata atcgctcatc accctcctcc
     4381 ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac
     4441 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg
     4501 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
     4561 ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg
     4621 ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt cggtcactat tgttagccag
     4681 cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgcagggg
     4741 atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt atacccgcgg attccagatc
     4801 gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac
     4861 gcactttctg atatggcact ctttgaacgc gtagaagtag tgcgtggcgc gacaggactc
     4921 atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt
     4981 cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg
     5041 gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc
     5101 taccagaata acgactcatg gctggaccgc tacaacagtg aaaagacctt cttctccggc
     5161 attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc
     5221 attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga tggcagcagc
     5281 aacagttacg atcgcgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc
     5341 aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacgctgaat
     5401 gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac
     5461 aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt tgattatgtc
     5521 ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac
     5581 ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa
     5641 caaaacaatc gttacttcag ttcatggacc aacatcttcc cggatgaaat tggcagtttc
     5701 tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct ggcgcaggac
     5761 gataccacac atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg
     5821 catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg
     5881 gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg
     5941 acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa
     6001 tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat
     6061 agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc
     6121 accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcagt ggatgggaca
     6181 gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca
     6241 tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg
     6301 ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca
     6361 gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc
     6421 accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg
     6481 acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc
     6541 aacgtggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat
     6601 caattctgaa taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt
     6661 cttacagatt agtggttacg gatgtactca tccatctcgg ttttcaggtt gtcggattta
     6721 gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc
     6781 ttcaggccag cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata
     6841 catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc
     6901 atttcgctgg tacctgtcgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc
     6961 agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata
     7021 ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc
     7081 agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc
     7141 aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac
     7201 tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg
     7261 cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc
     7321 agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc
     7381 atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc
     7441 tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg
     7501 atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag
     7561 gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg
     7621 gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca
     7681 ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc
     7741 cctccgagta cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc
     7801 agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg
     7861 gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc
     7921 agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg
     7981 ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg
     8041 gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac
     8101 ataattgaga gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg
     8161 ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat
     8221 aattcagtgc caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt
     8281 gcacattttg acaaaacata tgtcacaata tttcaaacgt ggttattcac cgcccaattt
     8341 tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa
     8401 cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt
     8461 acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca
     8521 cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag
     8581 tcagtttcca ccagtaatcg atccaggggg acataacgcg cagcatcgcg cagttgctcc
     8641 gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta
     8701 cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt
     8761 tcttcgcgca gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc
     8821 ttgttcagtt cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta
     8881 gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct
     8941 gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg
     9001 ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc
     9061 ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga
     9121 acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag
     9181 tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg
     9241 caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt
     9301 aacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga
     9361 gggagaaaga tggtttgcca gttcggcgac caggcccggc acatcaacat tggtcacctg
     9421 cgcagcacca tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg
     9481 cgccggagct tgttcatgat taagggccgc tagcagcgaa taccagtcgc ccgatggcac
     9541 gctatatgcc aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa
     9601 cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg
     9661 tgacattgtc acttcgcgtg aaagccaggt cacggcgtac tgttccggcg gcggcgcaag
     9721 gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc
     9781 caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc
     9841 ggcgtcggtt agtaaggcag catcggttac ccagacaact ttcgcaccac ctaagcgtgc
     9901 gtgctcattc agcttttcgg tgacctcacg taccgcatca atgcccaacg catttttccc
     9961 tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc
    10021 acgacagtga ccgcaacttt tgtgaccctg cggttgttgg cagagtaaat aacggctcag
    10081 ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagca gcgcatggtg
    10141 acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata
    10201 ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc
    10261 acggcctcca gcggctgggt ggcatcaatg gtatgaatgc ttttatcttg tgctgccagt
    10321 tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc
    10381 agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc
    10441 gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg
    10501 tcaataccac gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc
    10561 acccaggtgc cgttagccag cgcaggtttg atgactgttt ctaccagttg aacgcgcgcg
    10621 gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata
    10681 tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa
    10741 accatgtcgc ggatacccag ttgctcgagc gtctcaacca ccacattacg cgcggtagtt
    10801 ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag
    10861 cactttcaga taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg
    10921 accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc
    10981 cttcagcgaa tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata
    11041 ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc
    11101 tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt
    11161 gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat
    11221 tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc
    11281 ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc
    11341 ggtggtattg gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg
    11401 gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt
    11461 gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag
    11521 tcgcagcggg aactgtgctt ctttaccgct ttccaacaat ttcagcatct cgcgcacggt
    11581 catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg
    11641 cagcagccat tgaaaaaccc gtgggcgatt gatgatctta tcagcataaa gctgttcacc
    11701 gagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa
    11761 tttgctgtcg gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac
    11821 caccagcaat aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa
    11881 agtggggcta aatattcata taacgttgct gacgaaaagg agacatcgcc acaggcacgt
    11941 acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctct
    12001 tccagagagg cttgcacttc gacaagctga taagaggatt gtgccagcaa acggatacag
    12061 aattgtcgca taatgccgtt aacacctgcc tgatccagtc gcggcgtata aactacattg
    12121 cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg
    12181 accagcgcct catcagcgtt tgtctgctca agatgagagc ggatcaatac ttgctcaagc
    12241 cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc
    12301 gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataaac cgtaacggag
    12361 agaatccgcg ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc actaccgcga
    12421 ctgatcacga ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt
    12481 tcaagctgag gccagaagtc acagaaaatc atcaaccgct gacaagcatc ctgtagtcgc
    12541 tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa
    12601 ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt ataaccgtta
    12661 attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg
    12721 aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtgga
    12781 aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg
    12841 agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca
    12901 gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca
    12961 gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca
    13021 tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg
    13081 cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa
    13141 tctgactcgc ttcaatgcct gcatcacgca gagcatttgc cattgccaga gctgcgcctg
    13201 cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga
    13261 gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca
    13321 taccggcacc atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg
    13381 cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacgccca
    13441 gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga
    13501 taatacgcgc agcatgacca atgttgtgca cgccggaagt acaggcggtc gcgatagaga
    13561 tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg
    13621 ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt
    13681 tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg
    13741 cgttctcttc cgttatttca aggccagaat cctgcatggc ctgaacgcca gcgacaattc
    13801 catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt
    13861 taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga
    13921 ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc
    13981 cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc
    14041 tccagggagg gaaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg
    14101 accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa
    14161 cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag
    14221 ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat
    14281 tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt
    14341 cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg
    14401 tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta
    14461 cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat
    14521 aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca
    14581 tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga
    14641 atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca
    14701 ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg
    14761 gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga
    14821 agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac
    14881 caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta
    14941 ccgcttttga cagacggaaa acagatgaaa ggttggtttc gataatatcg ttccactctt
    15001 catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat
    15061 ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg
    15121 tcacattcaa catcagacct ttgccgttag cacctaaata atcactgatc gcctgagcgc
    15181 cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa
    15241 ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca
    15301 tgattttcct cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg
    15361 aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt tagcactttg cccgggccga
    15421 cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa
    15481 ccgggttata caactgacgt accagtgcgt cacgaatggc atcaccattg gtttcgcatt
    15541 tcacatcaac gttattcaca acaggaactg ttggtgcgtt aaaggtgatt ttcgctaatt
    15601 ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg
    15661 gtaacggcag cgcgcgtttt gcgcccgcag ctttacaggc agcgccagca cgctcaaccg
    15721 cctctttatg accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga
    15781 cctgaccttc tgcagcttct tcacacgctt tcgcaataga cgcatcatcc agaccgatga
    15841 ttgccgccat agcgcccgtg ccttccggta cggcttcttg catgaacttg ccgcgcatct
    15901 caaccagacg caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc gcggagtatt
    15961 cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc
    16021 ggtacagcgc aacagatgca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt
    16081 cttcagctgg cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt
    16141 cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg
    16201 tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct
    16261 agaaacgaac cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca
    16321 actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg
    16381 ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccata gacataccga
    16441 gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca
    16501 gttgagaacg gtcaagatta ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg
    16561 ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat
    16621 tcacgcggtc ggcgtttggc agcgtcagca attcaccata actaccgtcg gcatgcagat
    16681 gggtggagat gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc
    16741 caaaaataat aatagtccca cgatctgttg gatcgcaggt gcgcgccagt acatcggaac
    16801 cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg
    16861 cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca
    16921 acatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca
    16981 caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg
    17041 cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac
    17101 cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg
    17161 tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc
    17221 agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc
    17281 taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat
    17341 tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca
    17401 atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc
    17461 aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc
    17521 aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca
    17581 ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaacgtg
    17641 acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg
    17701 gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc
    17761 cgaatactgt cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga
    17821 ttgggaattt ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac
    17881 attgtgctgt cacaatcgac gttggcccct aaatcgagga ccaccgtttt gcccttttgc
    17941 tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat
    18001 aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga
    18061 ccttctttca ccagctccag ggccatacgc attgaactcc cacgactggc gcggatagct
    18121 tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa
    18181 cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa
    18241 agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa
    18301 gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg
    18361 ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc
    18421 agacgcatga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt
    18481 ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca
    18541 gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg
    18601 ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact
    18661 tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcgcttct tcaggcagtt
    18721 caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta
    18781 ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt
    18841 cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca
    18901 caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac
    18961 actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga
    19021 tagcgaacga catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc
    19081 gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa
    19141 ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta
    19201 acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac
    19261 attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg
    19321 aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc
    19381 taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat
    19441 ctcttttgaa tgtgcagcac cagaggtcga cgaaacccca cgcagcgacg aatcaccgcg
    19501 acagttggtg cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac gttatccgga
    19561 tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc
    19621 gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt
    19681 ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc
    19741 ttttgacgtc catttccgtc acctgagcga ggcggagatt gataattacg tgcgtaagga
    19801 gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga
    19861 gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca
    19921 gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc cggatgcggt
    19981 tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc
    20041 aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac
    20101 aacgctttaa accttcatcc atcggcgctt cgatacgcat cacttcaccg gtccccggat
    20161 gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag
    20221 tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg
    20281 catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca
    20341 gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct
    20401 gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa
    20461 cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc
    20521 ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc gctaccagca
    20581 acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc
    20641 gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg
    20701 taccggaagg tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca
    20761 gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa
    20821 ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt
    20881 tgttcacccg cacttcacct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt
    20941 tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa
    21001 caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat
    21061 tcgcgccact catttttcta tgcttatatt tagttttaca ccttattact tcactacgtg
    21121 atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa
    21181 gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg
    21241 ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg
    21301 accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct
    21361 gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt
    21421 tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct
    21481 ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca
    21541 cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt
    21601 acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta
    21661 gatgggcagc gtctgtatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca
    21721 aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat
    21781 tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct
    21841 gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa
    21901 gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt
    21961 atcagtctgg cgggtagcta tctggttctg atgccgaaca acccgcgcgc gggtggcatt
    22021 tctcgccgta tcgaaggcga cgaccgtacc gaattaaaag aagcactggc gagccttgaa
    22081 ctgccggaag gcatggggct tatcgtgcgc accgctggcg tcggcaaatc tgctgaggcg
    22141 ctgcaatggg atttaagctt ccgtctgaaa cactgggaag ccatcaaaaa agccgctgaa
    22201 agccgcccgg ctccgttcct gattcatcag gagagcaacg taatcgttcg cgcattccgc
    22261 gattacttac gtcaggacat cggcgaaatc cttatcgata acccgaaagt gctcgaactg
    22321 gcacgtcagc atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtac
    22381 accggcgaga tcccgctgtt cagccactac cagatcgagt cgcagatcga gtccgccttc
    22441 cagcgtgaag tccgtctgcc atccggtggt tccattgtta tcgacagcac cgaagcgtta
    22501 acggccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaaccgcg
    22561 tttaacacca acctcgaagc tgccgatgag attgctcgtc agctgcgcct gcgtgacctc
    22621 ggcggcctga ttgttatcga cttcatcgac atgacgcctg tacgccacca gcgtgcggta
    22681 gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt
    22741 tctcgctttg gcctgctgga aatgtcccgt cagcgcctga gcccatcact gggtgaatcc
    22801 agtcatcacg tttgtccgcg ttgttctggt actggcaccg tgcgtgacaa cgaatcgctg
    22861 tcgctctcta ttctgcgtct gatcgaagaa gaagcgctga aagagaacac ccaggaagtt
    22921 cacgccattg ttcctgtgcc aatcgcttct tacctgctga atgaaaaacg ttctgcggta
    22981 aatgccattg aaactcgtca ggacggtgtg cgctgtgtaa ttgtgccaaa cgatcagatg
    23041 gaaaccccgc actaccacgt gctgcgcgtg cgtaaagggg aagaaacccc aaccttaagc
    23101 tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct
    23161 gaacgtaagc gtccggaaca acctgcgctg gcaacctttg ccatgccgga tgtgccgcca
    23221 gcgccaacgc cagctgaacc tgccgcgcct gttgtagccc cagcaccgaa agctgcaccg
    23281 gcaacaccag caactcctgc acaacctggg ctgttgagcc gcttcttcgg cgcactgaaa
    23341 gcgctgttca gcggtagtga agaaaccaaa ccgaccgagc aaccagcacc gaaagcagaa
    23401 gcgaaaccgg aacgtcaaca ggatcgtcgc aagcctcgtc agaacaaccg ccgtgaccgt
    23461 aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac
    23521 cgtcgtaatc gtcgccaggc acagcagcag actgccgaga cgcgtgagag ccgtcagcaa
    23581 gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg tcgtgaacgt
    23641 agccgccgcc gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt
    23701 gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt
    23761 cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg
    23821 gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca
    23881 gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa
    23941 cagcaagaag agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc
    24001 cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt
    24061 tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct
    24121 ggcaaagtct ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag
    24181 cgcgaacagg aagaagtaca agtgcagccg atggtgactg aggtccctgt cgccgccgct
    24241 gtcgaacctg ttgttagcgc gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc
    24301 gtaccggttg ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc
    24361 gccgcggtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa
    24421 gtcgcagaac acgcagaacc ggtggttgaa ccgcaggaag agacggcaga tattgaagaa
    24481 gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag ttgttgctca acctgccgcg
    24541 ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtag ccgcggttga accagagatc
    24601 accgttgagc ataaccacgc taccgcgcca atgacgcgcg ctccggcacc ggaatatgtt
    24661 ccggaggcac cgcgtcacag tgactggcag cgccctactt ttgccttcga aggtaaaggt
    24721 gccgcaggtg gtcatacggc aacgcatcat gcctctgccg ctcctgcgcg tccgcaacct
    24781 gttgagtaat aattagctca aagtaatcaa gccctggtaa ctgccagggc ttttttattt
    24841 catgtttgaa tcaacatatt aaaattcatc aaatggcatc cttgctaacc aacaatgcaa
    24901 aataggcaaa aaaaatgccc gccccggcga tcccagagcg ggcagacaaa acatacccag
    24961 tttcatgata tgttttaaag cgagcttatt tgttgagctg gaagagcgac aatccctgca
    25021 tatcggtaaa tgctttatac gatgcctgca atgccgtttg ctgcatgatg taagatgaaa
    25081 tagttgcatt ccagtccaca tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc
    25141 gatcgctacc taaggaatcc agcgactcca gttcgttcag ctgcgtgcct aattccgcgc
    25201 gaacggtaag gacattattc agcgagtttt tcagtccacg gttggtttta tccaatgccg
    25261 cagcggcgat ttctttatcc gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg
    25321 cgctatccag catggcaaaa agattggttt cagaagcgct accgtctggt tccgctaccg
    25381 cgttactggt aatactgtcg aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg
    25441 aagcatcgac ctgttgccta atactttctg ctccaccgac gtatttccct ttctcttcgc
    25501 taaacggcgc agtctctgtt ttataaccgg caaaaatgta gcgcccgtta ccgtcagtgg
    25561 tgttcgccag attcagcaac tggtcacgaa gcccctggat atccgtagcc agcgaggccc
    25621 ggtcatcgtc actcaaagtg ccattgctgg cgtagacaat tttttcctga gcattctgga
    25681 tagcagtggt gacctggcta agtacactct cttccagtga cactttttga gtggcgaaag
    25741 tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca accgcttgtg
    25801 atgcagcaat gggatcatct gaagggttaa cgactcgctt acccgtcgac atctgttcgc
    25861 cgtacttcat ccattctgcc tgagaattgg tgatgccacg catgttttgc tggtacatca
    25921 tctgggtact gaaacgcatc ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat
    25981 cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag
    26041 atttccgtac tcctcatcga gattgacacc ggaaatcgac tgctgctgat tggaaagctg
    26101 cgtcaccaca ttaccttgcg tggtgctact ggttttcaac gtcgcggttt tattaccgat
    26161 atcactcact aacgaggcat aagcgtcgtt aaaggatttc gcaccgccca ccgttttact
    26221 gttgctttgc agatccagca gggcctgacc gttgcggttg tcgctatcac ccgcatcttc
    26281 ttcgctcgcc atcgcgattt tcgcttcgtc ggtgattaat acatccatgt tgacgatggc
    26341 gtcacttact ggtttcagcg tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa
    26401 ctccagacca tcaaatgcca ctttaccgtt ggcatccggc gtcaccgtaa aagtggtatt
    26461 gctggcaagg cgggtgacct gccactgatt attatcgaac gagattttgt aatctgtcgc
    26521 cagtaccccg gaggcatcag ttaccgtggc accgatcgca acgtcacctt tgtttttcgt
    26581 gttttgcaga atcgcgggct taccgatagc aaagaaatct tcaccagcat cgccgttggc
    26641 atcaaatccg gctttgtgtt gggtgttgaa agcctcggca aatgccagcg ccagttgtcc
    26701 aagcgtatta cgcgtctggt ccagatcctg agaacggaat gtcagaatgc cgcccagcga
    26761 cccggtattc agcaatttct ccgggatctc aatattgcct gccgtcccat caacgtaagc
    26821 gacagtcgta cgagaagggt cagcgctgga aggaactgcc gccagttgcc gagccgtact
    26881 tccctgaacc agtgagtaac cattggccat cgtgatgtta taagtgccgc catcctgaac
    26941 gctgacttct acaccaacaa tctggtttaa ttcgctcacc agttgatcgc gttgatccag
    27001 cagattgtta ggtgacgccc ctgcccccac gcctgtcagg cgcgagattt gatcgttcag
    27061 gctggcaatt tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt
    27121 gacctgtttg tcctggtcgc gaagatactg atcggtggtt ttaaactgat tcaccaaccc
    27181 ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct tccgcgttac tcaccagcgt
    27241 ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag
    27301 catattgtcg attttcgaca tctgctcata gcgggcagtc aggccgctac tttgcgtctg
    27361 cgccgcacgt aactggttgg taataaacgc atcatactca cgctgcacac cagaaacgtt
    27421 gacgccattg ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt
    27481 ggtttggcgg gtatatccag caacgttata gctggagata ttattacttg ccgtatttaa
    27541 cgccgcctgg gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt
    27601 tccttataag ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt
    27661 cagaacagat tatcaatgtt catactgtag gttttgctca ccttgtcgct tatcgatttc
    27721 atctgctgga tcatgttggt gagtttacgg gcatagtgag gatcggtggc atagcccgcg
    27781 tcctgtaggg cctgcgcccc ctgttccgca ctcgcggcgg tcgtcacggc agcgtagcgc
    27841 gggttacgtg ttaacaaccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc
    27901 cgaaacttcg cttttacttt cttcgcttcg ccgttttcat attcagtcgt ggtgatttca
    27961 gtaactggcc ctttccagtt gccagaggct ttgacaccaa acaggttata gcttggctcg
    28021 ccgttttcgc ggcggatttg ccgttgcccc caaccagatt caagcgccgc ctgagcgagg
    28081 atcaaatgat gtggcacacc gctttgctgg cttgccagtt gggcgggcag cgagagttgc
    28141 gcgaggaatg ctttactgtc acccggcagc gaatcatcgt agttacgtgg cacggccttt
    28201 tgcaccagct gcgaaagcgc ctgattttga taacgcacca cggtttcgag cgggaatttc
    28261 atcggtgctg ctggcgtggg ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc
    28321 atcatctctg caagccccag acctttgccc gccgtcatct gttgggcaat ctgctggtca
    28381 tacatactgg tatacaggcg agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg
    28441 tcgcgcatgc ttttcaacat catctgcacg aacatccctt ccacctgacg ggccaccgga
    28501 cggatatttg ccgccgggtc ttcgcccgct ttcgccttta gttcgttgag tgattgcgca
    28561 tcccaggccg cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg
    28621 cccgcagaca tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag
    28681 cgcccagcgc attgagcgcg cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt
    28741 gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg tggagtaacc acagtctgtc
    28801 caccaccaaa cggtgtatct ggctggctga cattggcctg acgattgact gttactgaga
    28861 gatttccctg cgctaccgcg cagctgtcga gggtcacttc gcgattcatc accaccgaac
    28921 cggtgcgcga gttaatcact actttagcgt cctgcggggt gacattaacc tgcatattct
    28981 ggatatctgc aaggaaacgg acctgggaac tgttgccact tggtacgcgc acctgaatag
    29041 tccgcgcatc taacgcggtg gcgctgccat atccacgcac gcggttgatg gtgtcagcga
    29101 tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa attaagggta ttcccgacgc
    29161 caaactggct gggcaattca cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca
    29221 gttggttcac ctgaacactg ctaccgccag cggaggctcc tgcgccgcca accagaatat
    29281 tgccctgcgc cagcgcatac acctgactgt caacgccctt aagcggcgtc atcaacaacg
    29341 tacctccacg caagcttttg gcatttccca tggaagaaac caccacatcg atggtttgcc
    29401 cctgacgtcc aaacggagga agtgacgctg tcaccattac cgcagcgacg ttttttagct
    29461 gcatattggt gcccgtcgga acggtaattc ccagctgtga gagcatgtta ttaagcgttt
    29521 gtgtggtaaa cggcgtctgg gttgtctggt caccggtgcc atccagcccc accaccagac
    29581 catagccaat cagtgagttt tgccttaccc cctgaacact ggtgagatcg cgaatacgct
    29641 cagcctgagc cgccgtcgta accagtagaa gaattaatgc agagagaaat ttaatcacca
    29701 cagcctcact tacattggcg acaggttaag gaagaaacgc tgcaaccagc ccatattttg
    29761 cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc gcatccgcca cctgagtaga
    29821 cggtacggta ttgctgccgc tgatagtgcg tggattaacc acgccagaga agcgaataaa
    29881 ttcggtaccc tgattaatgg caatctgttt ttcacccacc acatgcaggt tgccgttgac
    29941 cagtacctgg tcaaccgtca ccgtcaacgt gccgttaaag gtattgctgg cattagcccc
    30001 gccctttccg ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag cgttaccaaa
    30061 cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa ttagttttac cgtcacggct
    30121 ggcattcgca taggagcttt tgctggcgct gacgttctcc tgcaacacga tggtcagcgt
    30181 atcgccaata ttgcgtggtc gacgatcttc aaacagcggt tgatagccat agttaatcgg
    30241 ctgagcagac tggaaaatag aaccgttggc gacgggcgtc ggcccgggaa ccggttgtgc
    30301 actggtcgcc ccctgcacca gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag
    30361 caccaacaag ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg ctatcatctt
    30421 caaaaatcag taaaccggtg aacctgccac cggttaagcc ttagagttgc gtcagttttt
    30481 gcagcatctg atcggtggtg gacaccgctt tactgttgat ttcgtaagcg cgttgcacct
    30541 gaatcatatt gaccagttct tccgccacgt tgacgttaga cgtttcaaca tacccttgat
    30601 acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt cggtgcacca gaggattgcg
    30661 tttcggtgta gaggttttcg ccaatgctct ccagcccggt gtcattcata aaggtggtga
    30721 gattgagctg cccaacctga accggagctg cctggccttg ttgggttacg ctgaccacgc
    30781 catcacgacc gatggtgata cttaacgcat tcgccggaat ggtgatcgct ggctgcacct
    30841 gaaaaccacc agccgtcacc agctgcccgt tctgatccac ctggaaagag ccgtcacggg
    30901 tataggctga tgaaccatct ggcaacatca cctggaaaaa gccctgccct ttaatcgcga
    30961 catctttgct gttgttggtc tgcgacaggt ttccctggct gtgtaagcgt tcagtggcga
    31021 ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt tcggaagact
    31081 gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg cgctgacgct
    31141 taaaaccgtt agtactgacg tttgccaggt tgttggcaat gacgtccata ttggtttgct
    31201 gggcgtcaag gcccgttttg gcgatccata atgaactgat cataaaatgt cctgtagtgg
    31261 attagtgaca agcagagcag aagcacgcga cagtgtgaat ttcccctcac cctaaccctc
    31321 tccccaaagg ggcgagggaa tagtccgcgc gcttttattg caatgtcatc aatcgttcaa
    31381 ttaactcatc gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac
    31441 cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc
    31501 attgacgtta ctgccttcca gaacccccga catcacacgc aaggttggat ctgcctgcag
    31561 tatcggccca cgcgtggcct ggctttctgc gcttaaacga aaaatgccgt cgtcaccgcg
    31621 ttgcacttcg ctgcccgtgg ctttcaccag tttcagacgc cccactggcg caaccgtatt
    31681 tgccggatcg cccggattga gcgccgagat tgtgccatcg gcagcaatag tgatttccgc
    31741 cccttccggc acagcaattg gcccagcctc gcctatcacc ggatgcccct gaattgtcag
    31801 ttgcccggtg ggatcaacct gaatgctgcc attacgcgta tacccttcgc tgccgtcagc
    31861 ggactgcacg gccagccagc catcctgctg caatgcaacg tccagcgggc gcgaggtgta
    31921 atccatcttg ccgggcgtca tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt
    31981 gggcagtgaa agcccttcca ccggcactgc acgcaaggcg tttaactgcg cgcgaaaacc
    32041 gggcgtagag gcattggcca gattactggc ggttaccgcc tgttgattca gcgtctggct
    32101 ggctgctccc atcgcggtat aaattgcgtg atccattgag ctatcccgtc agcgattagc
    32161 gtaagttaac cagcgtgttg aggatctggt cctgggtttt gatggtctgg gcgttagact
    32221 gatagttacg ctgggcaacg atcatattga ccagttcttt actgagatcg acgttggacg
    32281 cttccaacgc accgttggtc agggtgccaa agttgcccgt cccggctgtc cccaacagcg
    32341 ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt
    32401 tgttggcaaa gttcgccagt acaatctgcc ccagcagttg tgtttgttcg ttggaatagt
    32461 tgccgacaac cgtaccgtca tcattgattt gataactcac cagatcgccc ggtttgtagc
    32521 cattctgggt ggttgccaca atattgttag cgccggtatt ttgctgcatg gagttgagga
    32581 agctcaggct aaacgtggcg ggatctgcgc cgttaattgc gccagtggta atattctctg
    32641 ttggatttga ggtcagaacg ccattggcat taaacaccag cttcattgca ggctcggctg
    32701 tacctgttgg atcactgcta tcctgggtgt agacctgcca gttattatcc ccggtcttca
    32761 caaagtagac gctcatgtca tgagcattac cctgactgtc gaaaacagtc accgaacctt
    32821 ttttgttata gctatccgca ttgctggcgc tgaatggcgt aacagtagga agcggatcgc
    32881 tggaattcag gttgatctgc atcgatgccg tggtggtagt tttcgctgcc atcagggtat
    32941 tcgggatcga aatattagtc ggattcgccc cttgctgaat agtcggcggc gtaccggttg
    33001 ccgggtaacc cgtcagctgt aaaccttgca tattcaccag gttacggttt tcatccagct
    33061 taaattgtcc gttacggctg tagaacaccg aaccgttgct atctaccaga cggaaaaaac
    33121 cgttctggct gatagcaacg tccagaccgc gcccggtgtt ggtggtcgtg ccatctgtaa
    33181 agtcctgagt gataccggca acttttaccc ccagtcccac tttcgaaccg gcaaacatat
    33241 cggcaaaaga ggccgtgcct gatttaaagc cgtaggtggc ggagttggcg atattgttgc
    33301 caataacatc gaggttagtg gcggcagcgt ttaatccgct aaccgcttga gaaaaggcca
    33361 tgactgactc ctgaaatgtg aaggcttaaa ttatctgccg tacttcgtcg agggtggtgg
    33421 tgccgtaagt gccgagatcc agcgtattac cgctgttgcc gcggatcaca ccctgcacca
    33481 gagcaaactg cagcggctgg gcaaccagtt gtgtaccacc gttactggcg ctaatcgcta
    33541 cattgtaaga accgttcggc gcagtgctgc catcagtcaa cgtaccgtcc caggtgaaac
    33601 tgtgaactcc ggcggtcagt tcaccaatat caatggtgcg cacaaccgcg ccatttttat
    33661 cggtgatggt ggccgtaact ttgtctgccg cctgttgcag ctcaacacca aacggcgtgg
    33721 tcgtggtcac agccccttct tcactgccgg ttcccgcaag aacagttgtg ccggggatca
    33781 tcacgccgtg accgatcagg ttactggcct gtaacgactg gctgttatca atctgtccgg
    33841 aaatagatcc gagcgtggta ttgagttttt caatcccgct gaccgtgctg atttgtgcca
    33901 attgcgacgt cagctcgttg ttttccattg gattggtcgg gtcctggttt ttcagctgcg
    33961 ccaccagcaa agtcagaaaa ctgctttgta aatctgcggc gttgctgccc gtgagcgaac
    34021 tactgctggt ggtactgacg ccggtatttg tcggatcggt ggtggttacc gcaatggaca
    34081 tagctttctc ctttattgac cgagcgtaag ggttttcagc atcatgcttt tcaccgtgtt
    34141 gagcacttca acgttggcct gatagctgcg tgacgccgac atggtgttaa ccatctcccc
    34201 gacgacatca acgttcggca tttttacgta gccctttgca tctgccagcg gattacccgg
    34261 ttcataaacc agtttgtccg gggcctgact ttctataaca tcggcaacct ttacgccgcc
    34321 tgtcgcagca cctggtgcag cgttaacctg gaataccacc tgttttgccc gatatggctg
    34381 tccatcagga ccggtcacgc tatcagcatt tgccagatta ctggccgcca cgttcaggcg
    34441 ctgggactgg gcagttaacg ccgacccggc gatatcaaaa atattcagca gtgccatccg
    34501 ttaatttccg ctctgtaaaa cgttcatcat gcctttgatt tgcccgctca acgcgctaag
    34561 gctcatctgg tattgcaggc tgttatcggc aaactgggtg cgttcgcgat ccatatcgac
    34621 ggtattaccg tcaagcgaag gctggtccgg aatacggtat tgcagttctg cggtaggagg
    34681 cgtcagcgcc tgcgccggga tgtgttgcgt tgaggtcatc gtcagtgcaa ccacactggt
    34741 tgcatcccgt ccacgttgca tgactttttt aagttcactg gcaaaatcga tatcgcgcgc
    34801 ctgataacca ggggtatcgg cattggcgat gtttgctgcc agcacttcct gacgctgggc
    34861 gcgcagattg agcgcctctt gttgaaaacg taaggcggcg tcgagcttat cgagcatatc
    34921 tcctccgcag gtatcaaaat tctgccatca gcttaaatgc ctttacgcgc gcgttatcgg
    34981 cggaataaac gcaaaatggg tcgctattta tgccgttgat ggtcattgcg gacaggtaca
    35041 attcacgttg tagaaatggc tgggggcgaa aatgctgaca ataaaacgta gcgtggcgat
    35101 catcgcgata ctgttcagtc cgttaagtac ggcgagcaat ctcacatcgc aattgcacaa
    35161 cttttttagc gcccaactcg cgggggtaag tgatgaggtt cgtgtttcta ttcgtacagc
    35221 gcccaatcta ctaccgccat gcgagcagcc attgctttcg atgagcaata attcccgcct
    35281 gtggggcaat gtgaatgtgt tggcacgctg cggtaacgac aaacgatatt tacaggttaa
    35341 tgtgcaggcc acaggaaatt atgtggttgc cgcgatgccc attgtgcggg gaggaaagct
    35401 ggaagctggc aatgtcaaac taaaacgcgg acggctggat accctgccac cgcgtacggt
    35461 gctggatatc aatcaacttg ttgatgccat tagcctgcgc gatctatcac ccgatcaacc
    35521 tatccagtta acccagtttc gccaggcatg gcgggtaaaa gcgggacaac gcgtcaatgt
    35581 gatcgccagc ggtgatgggt ttagcgccaa cgcagaaggt caggcgctga acaatgcagc
    35641 cgtcgcacag aatgcgcggg tgcgcatggt atcgggacag gtagtcagcg gcgttgttga
    35701 tgcagatggg aatattctta taaacctgta agctgttaaa gattacccgt cccttgccga
    35761 taaataagca acacatgata aaagcgccct caatgaggaa taaaccatga gtattgatcg
    35821 cacttcgcct ctgaagcctg taagcaccgt tcaaccgcgc gaaaccactg acgcgccggt
    35881 aacgaacagc cgggcggcaa aaacaaccgc ctccaccagc accagtgtga cgttaagcga
    35941 cgcgcaagca aaactgatgc aacccggcag cagtgatatc aatcttgaac gtgtcgaagc
    36001 gttaaaactg gcgattcgta acggtgaact aaaaatggac accggcaaaa ttgccgatgc
    36061 gctgatcaac gaagcgcagc aagacttgca gagtaactga tcgtatgaca cgtcttgcag
    36121 agatcctcga ccagatgtcc gctgtgctta acgatctcaa aacggtaatg gatcaagagc
    36181 agcaacatct ctctatgggg cagatcaacg gcagccagtt gcaatggatt acagaacaaa
    36241 aaagctcact gctggcgacg ctggattacc tcgaacagtt acgcaagaaa gaacccaata
    36301 cagcaaatag cgttgatatt agtcaacgct ggcaggaaat tactgtgaaa acgcagcaac
    36361 tacgccaaat gaatcaacat aacggctggt tactggaagg acagattgag cgcaatcaac
    36421 aggcgctgga aatgttgaaa ccgcatcagg aaccgacgct atatggggcg aacggtcaga
    36481 cctcaacaac ccatcgcggc ggtaaaaaga tttcgatctg aatattatct ccggcctgca
    36541 ctgcaggccg gaatgcattg ttacaccgtc cggcgggcaa attctttaac tttgaagccc
    36601 agtaccgcca gtgcagcgaa gtacgcggca atccccgcca gcacgaccgc cattaaacgc
    36661 agtaagcgcc agggcatggt acccaatgac cactccggca tgatatgtaa catacctaaa
    36721 agcacgccag acatcaccag taccgccacc accagacgca acagaaacgc catccagccg
    36781 ggttgcggag taaagatttt ctgcttacgc aactgccagt aaagcagcga agcattcaga
    36841 cacgccgcca gaccaataga aagtgacagc ccggcatgtt tcaacggacc aataaacgcc
    36901 aggttcatca attgcgttaa aatcagcgta acgatggcaa ttttcactgg cgttttaatg
    36961 tcctggcggg aataaaagcc aggagccaac actttcacta caatcaggcc aatcaaaccc
    37021 accgagtagg caattaacgc ccgctgggtc atcagcgcat caaacgcggt aaatttaccg
    37081 tactggaaca gcgaaacggt taacggacct gaaagaatgc ccaacgcaac cgcactcggc
    37141 agcgccaaca ggaaacaaag acgcaatccc cagtccatca aacggttgta ttcatcatga
    37201 ttgccactgg caaaactttt cgacagcgac ggcagcaaaa tggtgccaag cgccacgccc
    37261 agcacaccgg acggaaactc cattaagcgg tcggcgtaat acatccaaga caccgaaccg
    37321 gaagcaagaa acgaggcaaa aatggtgttg atgattaagg agatctggct cacagagacg
    37381 ccaaggatcg ccggtcccat ctgtttcacc acgcgcattg ctccggcatc gtggaagtta
    37441 atgcgcggca ggaccagcat gccgatcttc ttcaggtgcg gtagctgata caccagctgc
    37501 aggatgccgc cgaccgtaac agcccacgcc agcgccagca ccggtgggtt aaagtacggt
    37561 gcggcaaaca gcgcgaaacc aatcatgctg atgttaagca gtgttggagc aaacgccgga
    37621 atcgagaagc ggttccacgt attcagaatc gctcccacca gcgacgccag ggagatcagc
    37681 aagatatagg gaaaggtaat ctttagtagc tggctggtca gggcaaattt gtcagctgtg
    37741 tcagcgaagc ctggcgcggt caccatgatc acccacggtg cggcgagcat gccagcgacc
    37801 gtcacaaccg ccagcgcgag tgtcagcagg ccagaaacat aagagacaaa gacccgcgtg
    37861 gcgtcttcac cctgcttact tttatattcc gccagaatcg gtacaaatgc ctgggaaaat
    37921 gccccttcgg caaagatacg gcgtaacaag ttaggaagtt taaaagcgac gaaaaaggcg
    37981 tcggttgcca tccctgcgcc aaagattctg gcgacaattg cgtctcgtgc gaagccaagc
    38041 acacgcgaaa acatggtcat cgagctgacg gcggccagcg attttaataa attcatcggt
    38101 gttctaatcc agacccacgg gcaaaacgcc tgcaaggcag gcgctgaaag aggcgaatag
    38161 tctacctgga ttatggtgaa ttgctaccgc cagatgttac agggtttatt cactcatcgc
    38221 atcgcgccag atcttgtcaa cgatacgttg cgccagcacg gcctgttcgc cggcggtttg
    38281 cggaactgtc tgattttgca cacattcaat gaagtgccgc gcacagccga caaatccacg
    38341 ttgttcaaga gtgctctgcc aaccaggaat cggtttatgc actacgccct gcccgcgctc
    38401 ctcacgccat tcgcgcatat ccgtaatgtc gatgagcgca ccgtcagtca cggcctgcac
    38461 ggtttcacgc tgacttccgg cacggcgatg catacaggtg gtgatctgca aaggaccggc
    38521 cgaaaaatgg tgctcggcaa acagcatttc gccagcgtcg ttagtcagta gcgtaccgcc
    38581 atccagagag gctttgccgc ccgacaacca cagcgcggta tccaccacat gcagataatc
    38641 atccagcaac gtgaaataaa gatcgtgtgg cccgacacta ttgctacgat gtttatccat
    38701 tcttagcgaa gctgcggtag cgagttgcgt ttttaactca ccgtagagtg gtgcgaaacg
    38761 acggttaaaa ccgaccatca acgtcagttt tttccgcgcc gccagttcca ccagccgttc
    38821 agcatcgcgc agattttctg ccagcggttt atcgacacag acatgtacac ccgcattgag
    38881 taacgtactg accacgtcaa agtggctggc ggtgctggaa tgcacaaaaa ccgcatcgca
    38941 actggcggca aggctggata acgaatcggc ataaggaatg cgccagcttt cacaaattgg
    39001 cagggctttc gcgcgcgtag gcgaccaggc tccttgtaac atccagtcag acgctgccgc
    39061 cagcaccggt aaccacgctt tttgcgcaat gccacctaat cccactacgc cgatacgtaa
    39121 ttttttcact ttaatctccc agatggacca gcaacgaatc aagacgctgt ttcagttctg
    39181 ccacttcgat ttccagggct tcgacgcggg cctgtaaatc accatcaacc gcgttcgaca
    39241 tatccgtcac cgccggctga tcttcaacct caccactgaa aagatgcatg tagcggcttt
    39301 cgcgtttacc cggttcgcgg gccagacgca ccacaaaagg accatcttcg cgatttgcca
    39361 gttgttccag cgtcaactcc acttccgcca tatcgctgaa ttcatacatt cgcgcggcgc
    39421 ggctgcgcag ttcacccggc gtctgggcac cacgcaataa caacgtggtg attaacgcca
    39481 cttctgcggc gctcagtttc agatcgccaa attctgaatt acaaaaacgt tgctcatatt
    39541 tggtgacccg attaccaaaa ccgctcaccg tgcgtagata atgacgtttg accagattat
    39601 ccagctgttc ctgcacttca gattcactca gattcatgac cggttcacgg ttcgttttct
    39661 gattacaggc cgttactaca ccattgactg agagcgggta ttgctccggc gtggtcacct
    39721 gcttttccag caagcagcca atcacgcggg cttccagagc agtaagttga tatttcatga
    39781 gattctcctt agcggccggg cgtccagtct ggggtagtta atgccgtcag tacgtgatcg
    39841 cgccattgtc catcaatcaa cagatagtct ttcgcatagc cttctttttc aaaacccagt
    39901 cgcgccagta aatcaccgct gcgtttattg tgcggcatat aattagccat aatgcgatga
    39961 atatgttggg tgcgctgcat ataacgaatg gctgcggtca gggcttcaaa catgagtcct
    40021 ttgccctgcc atttttgccc aatcgaataa ccgagatagc aggcatgaaa agagccgcga
    40081 acaacattgg aaaaattggc aacgccaata atctcttttt cgtccgggtc gaataaacca
    40141 aagtagaaag ctgaaccttg tttatgaaat tcgttaatca tccccagcct ggcctgccag
    40201 cctgatggat aacagtggct ttcgtcgcgc actggctccc agggcttgag gaaatggcga
    40261 ttctctgcgt aataatccgc aagacgccag gcatcacgat catgcaccag acgcacgacc
    40321 agtcggtctg tggttaagcg cacttttggc acgttactgc gatagccaaa cattctatac
    40381 ctactccttc ccgtaacgtc tttaatacgc tgtaagtatt actatacctg cgcaaccaga
    40441 ccctgtgaaa agtccaaaat gccattttct gcattattca catatttgat aaaaactctc
    40501 tatcaaagcg acgttttgat aaaaaaatat tgtccccgaa gtgatgggaa aagtcttagc
    40561 ggcaatgcag aatagaaagt gctcatcttt tatttcccgg gaggggaaat gtcccgcgtg
    40621 tcgcaggcga ggaacctggg taaatatttc ctgctcatcg ataatatgct ggtcgtgctg
    40681 gggttctttg ttgtcttccc gctgatctct atccgcttcg ttgatcaaat gggctgggcc
    40741 gccgtcatgg tcggtattgc tctcggtcta cgccagttta ttcagcaagg tctgggtatt
    40801 ttcggcggcg caattgccga ccgctttggt gccaaaccga tgattgttac cggtatgctg
    40861 atgcgcgctg ccggattcgc cacaatgggt atcgcccacg aaccgtggct attgtggttt
    40921 tcatgcctgc tctcgggact cggcggcacg ttgtttgatc cgccgcgttc ggcgctggtg
    40981 gtgaaactaa tccgtccaca acaacgtggt cgttttttct cgctgttgat gatgcaggac
    41041 agtgccggtg cggtcattgg cgcattgttg gggagctggc tgttgcaata cgactttcgc
    41101 ctggtctgcg ccacaggggc cgttttgttt gtgctgtgtg cggcgttcaa tgcgtggttg
    41161 ttaccggcat ggaaactctc caccgtacgc acgcccgttc gcgaaggcat gacccgcgtg
    41221 atgcgtgaca agcgttttgt cacctatgtc ctgacgctgg cgggttacta catgctggct
    41281 gtacaagtga tgctgatgct gccaattatg gtcaacgacg tggctggcgc accctctgcc
    41341 gttaaatgga tgtatgccat tgaagcgtgt ctgtcgttaa cgttgctcta ccctatcgcc
    41401 cgctggagtg aaaagcattt tcgtctggaa caccggttga tggctgggct gttgataatg
    41461 tcattaagca tgatgccggt gggcatggtc agcggcctgc aacaactttt caccctgatt
    41521 tgtctgtttt atatcgggtc gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg
    41581 ctggcggacg caagagctcg cggcagctat atggggttta gccgtctggg tctggcgatt
    41641 ggcggcgcta ttggttatat cggtggtggc tggctgtttg acctgggcaa atcggcgcac
    41701 cagccagagc ttccgtggat gatgctggga attattggca tcttcacttt ccttgcgctg
    41761 ggttggcagt ttagtcagaa acgcgccgcg cgtcgtttgc ttgaacgcga cgcctgattt
    41821 gatactcccc cctgactcct ccatactgaa agtgaactga cagccaaatg gaggagtcac
    41881 gtgaagctat acatttacga tcactgccct tactgcctca aagcccgcat gattttcggc
    41941 ctgaaaaata tccccgtcga attacatgtt ctgctcaacg acgacgcaga aacacccacc
    42001 cggatggtcg gtcaaaaaca ggttcccatt ctgcaaaaag atgacagccg ctatatgcca
    42061 gaaagcatgg acatcgttca ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc
    42121 aaacgttccc ctgccattga agagtggctg cgcaaggtca atggctacgc aaacaaactg
    42181 ctgttgccgc gttttgccaa atcggcattc gatgagtttt ctactcccgc cgcgcgcaaa
    42241 tatttcgtcg acaagaaaga ggccagcgcg ggtaattttg ccgacctgct ggcccactct
    42301 gacggtctga ttaagaatat cagcgatgat ttacgtgcgc tggacaaact gatcgtcaaa
    42361 ccgaacgccg tgaatggcga actttcggaa gatgatattc agctatttcc gctactgcgt
    42421 aatctgacgc tggtagccgg aatcaactgg ccaagccgcg ttgctgatta ccgcgataat
    42481 atggcgaaac agacacaaat caatttgtta tcatcaatgg cgatttaatc ctgacccgcc
    42541 gcggcagcct gtattcgcgg gttgccgccg ttttcagttt tttctgctgg cgagtttatc
    42601 gctggtgaca catgctgcgt tgtgtcagga tattgacgca gatttcatgt cgaggactac
    42661 catgaacaag tttttatttg ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa
    42721 tcaactcacc caatacacca tcaccgaaca agaaattaac cagtcgcttg cgaaacataa
    42781 taatttctca aaagatatcg gtttacccgg cgtggctgac gcccatattg ttctgacaaa
    42841 cttgaccagc caaattggtc gcgaagagcc gaataaggtt accctaaccg gagacgccaa
    42901 tctggacatg aactccctgt tcggtagtca gaaagcgacc atgaaactga agctgaaagc
    42961 gctgcccgtg tttgataaag aaaaaggtgc gatcttcctg aaagagatgg aagtggtcga
    43021 tgcgacggta caaccggaaa aaatgcaaac ggtgatgcaa acgttgcttc cctatttgaa
    43081 ccaggcatta cgcaattact ttaaccagca acctgcttac gtcctgcgcg aagatggcag
    43141 tcagggcgaa gcaatggcga aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat
    43201 tgtcattccg tttacggatt aatgacgagg gcgcattcgc gccctttatt tttcgtgcaa
    43261 aggaaaacgt ttccgcttat cctttgtgtc cggcaaaaac atcccttcag ccggagcata
    43321 gagattaatg actgcaccat cccaggtatt aaagatccgc cgcccagacg actggcacct
    43381 tcacctccgc gatggcgaca tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg
    43441 acgggctatc gtaatgccca atctggctcc gcccgtgacc acagttgagg ctgccgtggc
    43501 gtatcgccaa cgtattcttg atgccgtacc tgccgggcac aatttcaccc cattgatgac
    43561 ctgttattta acagattcgc tggatcctaa tgagctggag cgcggattta acgaaggcgt
    43621 gttcaccgct gcaaaacttt atccggcaaa cgcaaccact aactccagcc acggcgtgac
    43681 gtcaattgac gcaatcatgc cggtacttga gcgcatggaa aaaatcggta tgccgctact
    43741 ggtgcatggt gaagtgacac atgcagatat cgacattttt gatcgtgaag cgcgctttat
    43801 agaaagcgtg atggaacctc tgcgccagcg cctgactgcg ctgaaagtcg tttttgagca
    43861 catcaccacc aaagatgctg ccgactatgt ccgtgacgga aatgaacggc tggctgccac
    43921 catcactccg cagcatctga tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc
    43981 gcacctgtat tgtttaccca tcctcaaacg caatattcac caacaggcat tgcgtgaact
    44041 ggtcgccagc ggttttaatc gagtattcct cggtacggat tctgcgccac atgcacgtca
    44101 tcgcaaagag agcagttgcg gctgcgcggg ctgcttcaac gccccaaccg cgctgggcag
    44161 ttacgctacc gtctttgaag agatgaatgc tctgcagcac tttgaagcat tctgttctgt
    44221 aaacggcccg cagttctatg gcttgccggt caacgacaca ttcatcgaac tggtacgtga
    44281 agagcaacag gttgctgaaa gcatcgcact gactgatgac accctggtgc cattcctcgc
    44341 cggggaaacg gtacgctggt ccgttaaaca ataaaaatcg tagcttcctg ttgtcattag
    44401 gttattttac ctgtataaat aaccagtata ttcaacaggg ggctattatg cgaattgaag
    44461 tcaccatagc gaaaacttct ccattgccag ctggggctat tgacgccctg gctggcgaac
    44521 tttcccgccg tattcagtat gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg
    44581 ccgcagcgaa taatttatcg gttattggcg caacaaaaga agataaacag cgcattagcg
    44641 aaattctcca ggaaacgtgg gaaagcgccg atgactggtt tgtcagcgaa taatatgcag
    44701 tgatttttgt ttttgccggg tcgccccggc ttttttattt tacttacaaa ttttgagtac
    44761 aaaatttcta cacttcctca aaataattgc acaatatgct aaaaaaatat gaatgacatg
    44821 ctgttttatt gttcatataa atcttaaaag cagaaattta tctgttcccc cctttaaaaa
    44881 ttcggtgaat acccttactt attggtatac tgaagttgct tcagataaaa acgcattgaa
    44941 cctcgaataa cgttgtctag taacacgaat tagggggcca tgatggaaaa aaataatgaa
    45001 gtcattcaga ctcatccgct cgtagggtgg gacatcagca ccgttgatag ctatgatgcg
    45061 ctgatgttgc gtttgcacta ccagacccca aataagtccg agcaggaagg gactgaagtt
    45121 ggtcagacgc tctggttaac cactgatgtt gccagacaat ttatttcgat attagaagca
    45181 ggaatcgcca aaattgaatc cggtgatttc ccggtaaacg agtatcggcg tcattaacat
    45241 gacatgacca tccacccaaa aggcacctca ccggtgcctt taccatttct gggttgtata
    45301 tctgcattca gttaattact ctgcttaaac catgagagca gagagaaaaa aatgaaatac
    45361 gatctcatca ttattggcag cggttccgta ggcgctgccg ccgggtatta tgcaacccgc
    45421 gccggtttaa acgtgctaat gaccgacgcc catatgccac cgcatcaaca cggcagccac
    45481 cacggcgata cgcgattaat tcgccatgct tatggtgaag gcgaaaagta tgtcccgttg
    45541 gtcctccgcg cgcaaacgct gtgggatgaa ctttcccgcc acaacgaaga tgatcccatt
    45601 tttgtacgct ctggtgtcat taacctcggc ccggctgact ccgcatttct cgccaacgtc
    45661 gcccacagcg ccgaacagtg gcaactcaac gttgaaaagc tcgatgcgca agggattatg
    45721 gcccgctggc cagaaatacg cgtcccggac aactacatcg gcttatttga gactgattcc
    45781 ggttttttgc gcagcgaact ggcgattaaa acctggatcc aactggcgaa ggaagcgggc
    45841 tgtgcgcaac tgttcaactg cccggtcacc gcaattcgtc atgacgatga tggcgtaact
    45901 attgaaaccg ttgacggtga gtatcaggcg aaaaaagcga ttgtctgcgc gggaacatgg
    45961 gtaaaagacc tgctcccgga gctgcctgtc cagcccgtac gtaaagtatt tgcctggtat
    46021 caggccgatg gccgctatag cgtgaagaat aaattcccgg cgtttaccgg tgaactgccc
    46081 aatggcgatc aatattatgg ttttccggca gaaaacgacg cgttgaagat tggcaaacat
    46141 aacggaggcc aggttatcca ttcagcggat gaacgtgttc cgtttgcgga agtggtcagc
    46201 gatggttcgg aagccttccc gttcttgcgc aatgtattgc cgggtatcgg ttgctgcctg
    46261 tacggcgctg cctgcaccta tgataattcg cctgacgaag attttattat cgatacccta
    46321 cccggccacg ataatacact gctcattacc ggcctgagtg ggcacggttt taaatttgcg
    46381 tcagttttag gggaaatagc tgccgatttt gcgcaagaca aaaaaagcga ttttgatttg
    46441 acgccattca ggctttcccg cttccaataa tcatcattac ggcctccaga atgaggccgt
    46501 ttttacagga gtatttaatg cgtcggctgt tgcactatct catcaataat attcgcgagc
    46561 atctgatgct ttatcttttt ctctggggat tgttggcaat tatggatctg atctatgtgt
    46621 tttattttta gttttccgca ctgacataaa ctgacaaata tttaatcccc ctttcattta
    46681 gcgtgaaaaa ttgaatgaga ttattcctgc ttctacagca ctcaccagac at
//
LOCUS       DAPHDX010000018        45816 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPHDX010000018 DAPHDX010000000
VERSION     DAPHDX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.018"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            191..817
                     /locus_tag="RH058_000987"
     CDS             191..817
                     /locus_tag="RH058_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV3136612.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            838..2940
                     /locus_tag="RH058_000988"
     CDS             838..2940
                     /locus_tag="RH058_000988"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV3136613.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2953..3591
                     /locus_tag="RH058_000989"
     CDS             2953..3591
                     /locus_tag="RH058_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV3136614.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3655..4323
                     /locus_tag="RH058_000990"
     CDS             3655..4323
                     /locus_tag="RH058_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV3136615.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4320..5105
                     /locus_tag="RH058_000991"
     CDS             4320..5105
                     /locus_tag="RH058_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV3136616.1"
                     /translation="MNSSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5109..5921
                     /locus_tag="RH058_000992"
     CDS             5109..5921
                     /locus_tag="RH058_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136617.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERALVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5933..7537)
                     /locus_tag="RH058_000993"
     CDS             complement(5933..7537)
                     /locus_tag="RH058_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV3136618.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7663..7968)
                     /locus_tag="RH058_000994"
     CDS             complement(7663..7968)
                     /locus_tag="RH058_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV3136619.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8076..8152)
                     /locus_tag="RH058_000995"
     tRNA            complement(8076..8152)
                     /locus_tag="RH058_000995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8116..8118),aa:Val,seq:gac)
     gene            complement(8157..8233)
                     /locus_tag="RH058_000996"
     tRNA            complement(8157..8233)
                     /locus_tag="RH058_000996"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8197..8199),aa:Val,seq:gac)
     gene            8541..9797
                     /locus_tag="RH058_000997"
     CDS             8541..9797
                     /locus_tag="RH058_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3136620.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9838..11211)
                     /gene="mdtK"
                     /locus_tag="RH058_000998"
     CDS             complement(9838..11211)
                     /gene="mdtK"
                     /locus_tag="RH058_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV3136621.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11426..12067
                     /gene="ribE"
                     /locus_tag="RH058_000999"
     CDS             11426..12067
                     /gene="ribE"
                     /locus_tag="RH058_000999"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV3136622.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12107..13255)
                     /gene="cfa"
                     /locus_tag="RH058_001000"
     CDS             complement(12107..13255)
                     /gene="cfa"
                     /locus_tag="RH058_001000"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV3136623.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13546..14757)
                     /locus_tag="RH058_001001"
     CDS             complement(13546..14757)
                     /locus_tag="RH058_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV3136624.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14870..15802
                     /locus_tag="RH058_001002"
     CDS             14870..15802
                     /locus_tag="RH058_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136625.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15799..16824)
                     /gene="purR"
                     /locus_tag="RH058_001003"
     CDS             complement(15799..16824)
                     /gene="purR"
                     /locus_tag="RH058_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV3136626.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17123..17212
                     /gene="ynhF"
                     /locus_tag="RH058_001004"
     CDS             17123..17212
                     /gene="ynhF"
                     /locus_tag="RH058_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV3136627.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17378..18547
                     /locus_tag="RH058_001005"
     CDS             17378..18547
                     /locus_tag="RH058_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3136628.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18693..19274)
                     /gene="sodB"
                     /locus_tag="RH058_001006"
     CDS             complement(18693..19274)
                     /gene="sodB"
                     /locus_tag="RH058_001006"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV3136629.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19402..20217)
                     /gene="mepH"
                     /locus_tag="RH058_001007"
     CDS             complement(19402..20217)
                     /gene="mepH"
                     /locus_tag="RH058_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV3136630.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20552..20899
                     /gene="grxD"
                     /locus_tag="RH058_001008"
     CDS             20552..20899
                     /gene="grxD"
                     /locus_tag="RH058_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV3136631.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20950..25566)
                     /locus_tag="RH058_001009"
     CDS             complement(20950..25566)
                     /locus_tag="RH058_001009"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV3136632.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAITGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25659..26306)
                     /gene="rnt"
                     /locus_tag="RH058_001010"
     CDS             complement(25659..26306)
                     /gene="rnt"
                     /locus_tag="RH058_001010"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV3136633.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26409..26816)
                     /gene="gloA"
                     /locus_tag="RH058_001011"
     CDS             complement(26409..26816)
                     /gene="gloA"
                     /locus_tag="RH058_001011"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV3136634.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26897..27994)
                     /gene="nemA"
                     /locus_tag="RH058_001012"
     CDS             complement(26897..27994)
                     /gene="nemA"
                     /locus_tag="RH058_001012"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV3136635.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28031..28630)
                     /gene="nemR"
                     /locus_tag="RH058_001013"
     CDS             complement(28031..28630)
                     /gene="nemR"
                     /locus_tag="RH058_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV3136636.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIDLLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28733..28972
                     /locus_tag="RH058_001014"
     CDS             28733..28972
                     /locus_tag="RH058_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV3136637.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29021..29917
                     /locus_tag="RH058_001015"
     CDS             29021..29917
                     /locus_tag="RH058_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV3136638.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29998..30519
                     /gene="sodC"
                     /locus_tag="RH058_001016"
     CDS             29998..30519
                     /gene="sodC"
                     /locus_tag="RH058_001016"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV3136639.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30520..32532)
                     /locus_tag="RH058_001017"
     CDS             complement(30520..32532)
                     /locus_tag="RH058_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3136640.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32532..33389)
                     /locus_tag="RH058_001018"
     CDS             complement(32532..33389)
                     /locus_tag="RH058_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3136641.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33392..33628)
                     /locus_tag="RH058_001019"
     CDS             complement(33392..33628)
                     /locus_tag="RH058_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV3136642.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33829..34263
                     /gene="slyA"
                     /locus_tag="RH058_001020"
     CDS             33829..34263
                     /gene="slyA"
                     /locus_tag="RH058_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV3136643.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34310..34777)
                     /gene="slyB"
                     /locus_tag="RH058_001021"
     CDS             complement(34310..34777)
                     /gene="slyB"
                     /locus_tag="RH058_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV3136644.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35051..36160
                     /gene="anmK"
                     /locus_tag="RH058_001022"
     CDS             35051..36160
                     /gene="anmK"
                     /locus_tag="RH058_001022"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV3136645.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36258..36587
                     /gene="mliC"
                     /locus_tag="RH058_001023"
     CDS             36258..36587
                     /gene="mliC"
                     /locus_tag="RH058_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV3136646.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36646..37302
                     /gene="pdxH"
                     /locus_tag="RH058_001024"
     CDS             36646..37302
                     /gene="pdxH"
                     /locus_tag="RH058_001024"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV3136647.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37431..38705
                     /gene="tyrS"
                     /locus_tag="RH058_001025"
     CDS             37431..38705
                     /gene="tyrS"
                     /locus_tag="RH058_001025"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV3136648.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38764..39627
                     /gene="pdxY"
                     /locus_tag="RH058_001026"
     CDS             38764..39627
                     /gene="pdxY"
                     /locus_tag="RH058_001026"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDV3136649.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39671..40276)
                     /gene="gstA"
                     /locus_tag="RH058_001027"
     CDS             complement(39671..40276)
                     /gene="gstA"
                     /locus_tag="RH058_001027"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV3136650.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40382..41884)
                     /gene="dtpA"
                     /locus_tag="RH058_001028"
     CDS             complement(40382..41884)
                     /gene="dtpA"
                     /locus_tag="RH058_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV3136651.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42495..43130)
                     /gene="nth"
                     /locus_tag="RH058_001029"
     CDS             complement(42495..43130)
                     /gene="nth"
                     /locus_tag="RH058_001029"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV3136652.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43130..43825)
                     /gene="rsxE"
                     /locus_tag="RH058_001030"
     CDS             complement(43130..43825)
                     /gene="rsxE"
                     /locus_tag="RH058_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV3136653.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43829..44449)
                     /gene="rsxG"
                     /locus_tag="RH058_001031"
     CDS             complement(43829..44449)
                     /gene="rsxG"
                     /locus_tag="RH058_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV3136654.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44453..45511)
                     /gene="rsxD"
                     /locus_tag="RH058_001032"
     CDS             complement(44453..45511)
                     /gene="rsxD"
                     /locus_tag="RH058_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV3136655.1"
                     /translation="MVFRIASSPYIHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45512..>45816)
                     /locus_tag="RH058_001033"
     CDS             complement(45512..>45816)
                     /locus_tag="RH058_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136656.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT        11524 a        11333 c        11958 g        11001 t
ORIGIN      
        1 cagaataatc acattatttt ttctcctaac cataaaggat tagtttatcg gcgttaaatc
       61 ttttctcata cactatcggg ttagggaaaa tatctggtgt tggggacaga gtcagcagcg
      121 tattagttga atgcttaata tcaggctgct gaattgtcag cactgtgttt gcgaagagga
      181 tgcgaaataa atgaacccgg ttgatcgtcc actattagat attggcttaa cgcgattaga
      241 attcttgcgt atatcaggaa aaggccttgc tggtctaaca attgcccctg cgttactgtc
      301 acttttgggt tgcaaacagg aagatattga tagcggcaca gtagggttga taaatacacc
      361 caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt tgccaccgct gtgaaatctc
      421 ttgtaccaac ttcaacgatg gctcagtagg gacattcttc tcccgtatca aaatccaccg
      481 caattatttc tttggcgaca acggggttgg ctctggcggc ggcctgtatg gcgatctcaa
      541 ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa tgcatgaacg tctgcccgat
      601 tggtgcgatt acctggcagc agaaagaagg ctgtattacc gtcgatcata aacgctgtat
      661 tggctgtagc gcctgtacta cggcatgccc gtggatgatg gccaccgtaa ataccgaaag
      721 taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca aacgcctgcc caacaggggc
      781 gttaaaaatt atcgagtgga aagatattac tgtgtgaatc ttgcaaagga aaaaatcatg
      841 gctaacggtt ggacaggtaa tatattaaga gtcaatctca cgacaggaaa tattaccctc
      901 gaagattcca gtaagtttaa aagttttgtc ggtggcatgg gcttcggcta caaaattatg
      961 tatgacgaag taccgccagg cacgaaacct ttcgatgaag cgaataaatt agtctttgct
     1021 accggcccat taactggatc tggtgccccc tgtagttctc gcgtaaatat cacctcactt
     1081 tctactttta ccaaaggaaa tttagttgtc gatgcccata tgggtggctt ttttgcagcg
     1141 caaatgaaat tcgctggata cgacgtcatt attatcgaag ggaaggcgaa atcaccggta
     1201 tggctgaaga ttaaagatga caaagttagc ctggaaaaag ccgatttctt atggggaaaa
     1261 gggacgcgcg caacgacgga agaaatttgt cgattgacca gtccggaaac ctgtgtggcg
     1321 gctattggtc aggctgggga aaaccttgtt cctctctctg gcatgttgaa tagccgtaac
     1381 cacagcggcg gtgcgggaac tggcgcaatt atgggttcga aaaacctgaa agcgattgcg
     1441 gttgaaggga cgaaaggggt caacattgcc gatcgtcagg agatgaagcg tctcaacgat
     1501 tacatgatga ctgaacttat tggtgcgaat aacaaccatg tcgtgccaag tacgccgcaa
     1561 tcgtgggcag agtattcaga tcccaagtca cgttggacag cacgtaaagg gctgttttgg
     1621 ggcgcggctg aaggtggtcc gattgaaacg ggggaaattc cgccaggcaa tcagaacacg
     1681 gtcggctttc gtacctataa atccgttttt gacttaggtc cggcggcaga gaaatacaca
     1741 gtaaaaatga gtggctgcca ctcttgcccg atccgttgta tgacccaaat gaatattcct
     1801 cgggtgaaag agtttggcgt gcccagcaca ggtggtaaca cttgtgtagc caactttgtc
     1861 cataccacca tcttcccgaa cggtccgaaa gattttgaag ataaagacga tggccgtgtg
     1921 attggtaacc tggtgggtct gaatttgttc gatgactacg gcctatggtg taactacggg
     1981 cagttgcatc gcgactttac ctattgttac agcaaagggg tgttcaagcg tgttctgcct
     2041 gctgaagagt atgcggaaat tcgctgggat caactggaag cgggtgacgt taacttcatt
     2101 aaagattttt actaccgtct ggcgcatcgt gtgggtgagc tgagtcacct ggctgatggt
     2161 tcatatgcca tcgcagaacg ctggaatttg ggtgaagagt actggggcta cgcgaaaaat
     2221 aaactctggt cgccgtttgg ctatccggtt caccatgcca atgaagcgtc agcgcaggtc
     2281 ggttccattg ttaactgtat gttcaaccgt gactgcatga cgcataccca tatcaatttt
     2341 attggttcgg gtttgccatt gaaactgcag cgtgaagtgg cgaaagagct ttttggttct
     2401 gaagatgcct acgatgaaac caaaaactac acgccaatca atgacgcaaa aattaaatat
     2461 gcaaagtggt cgctgttgcg ggtctgtttg cataacgccg tcaccctgtg caactgggtc
     2521 tggccaatga ccgtttcgcc gctgaaaagc cgtaattacc ggggcgatct ggcgcttgaa
     2581 gccaaattct tcaaagcgat caccggcgaa gagatgactc aggaaaaatt agatttagct
     2641 gcagagcgta tctttacgtt gcatcgtgcc tacacggtaa aactgatgca aaccaaagat
     2701 atgcgtaatg aacacgatct tatctgttcc tgggtattcg acaaggatcc gcagatcccg
     2761 gtctttactg aaggtactga caaaatggat cgtgacgata tgcatgcttc gctcacgatg
     2821 ttctacaaag aaatgggctg ggacccacag cttggttgtc cgacccgcga aacattgcag
     2881 cgtctggggc tggaagatat tgccgccgat ctggcagcac acaatctact gcctgtgtaa
     2941 ggaatgggtg agatgaacca tcgggatgaa ttgccgttag cgaaagtcag cgaagttgac
     3001 gaggcgaagc ggcaatggtt acaggggatg cgtcaccccg ttgatacggt aaccgaacca
     3061 gaaccggctg aaatcctggc agagttcatt cgccagcatt ctgcggctgg gcagctggtg
     3121 gcgcgcgccg tgtttctctc gccaccctat tcggttgcag aagaggagct gtcagttttg
     3181 ctggaaagca ttaagcagaa cggagactac gcggatatcg cttgtatgac gggaagccag
     3241 gatgattact attactcaac gcaggccatg agtgaaaact acgcggcgat gtctttgcaa
     3301 gttgtggagc aggacatttg tcgcgccatt gcacacgccg ttcgttttga gtgccagaca
     3361 tatcctcgtc cttacaaagt cgccatgctg atgcaagcgc catattactt ccaggaagcg
     3421 cagattgaag ccgcgatcgc cgcgatggat gttgcacctg aatatgccga tatacggcag
     3481 gtggagtcat cgacggcggt gttgtacttg tttagcgagc gatttatgac ctatggcaag
     3541 gcttatggct tatgcgaatg gttcgaggta gagcagtttc agaacccctg aaatttcagg
     3601 gagatgtcat ggatagggtg gacagttgca gccactgcat taggggataa tcagatgtcc
     3661 ttcactcgac gcaaatttgt tctggggatg ggaacagtaa tcttttttac tggttctgcg
     3721 tcttctctgt tagcgaacac gaggcaagaa aaggaagttc ggtatgccat gattcatgac
     3781 gagtcacggt gcaatggctg taatatttgc gcccgcgcat gccgtaaaac taaccacgtc
     3841 cccgctcagg gaagccgttt atcgatagca cacattccag tcaccgataa cgacaacgaa
     3901 acgcagtatc acttttttcg ccagtcatgt cagcactgcg aagatgctcc gtgcatcgac
     3961 gtatgcccga caggggcgtc gtggcgtgat gagcagggga tcgtgcgggt agaaaaatcg
     4021 cagtgcatcg gctgcagtta ttgcattggc gcttgtccgt accaggttcg ctaccttaac
     4081 cctgtcacta aagtggcaga taaatgtgat ttctgcgctg agtcccgact ggcaaaaggc
     4141 tttccgccca tttgcgtcag cgcctgtccg gaacacgcat tgatctttgg tcgcgaagat
     4201 agcccagaaa ttcaggcgtg gttgcaggat aataaatact atcaatatca gctacctggt
     4261 gccgggaaac cgcatctgta tcgccggttc ggtcaacatt tgattaaaaa ggaaaatgta
     4321 tgaactcgtc gcaacatgct gaacagtttc agagccagtt agcgaactat gtgccacagt
     4381 tcactcccga attttggccg gtgtggttga tcattgccgg agtattgctg gttgggatgt
     4441 ggttggtgct ggggctgcac gccttgcttc gtgctcgtgg cgtgaagaaa tcagccaccg
     4501 atcatggtga gaaggtttat ctttacagca aagcggtcag attatggcac tggtcgaatg
     4561 cgttactctt tgtattgttg ctggcaagtg ggctgataaa tcactttgcg atggtgggcg
     4621 caactgcggt taaaagtctg gttgcggtgc atgaagtttg cggatttttg ttactggcat
     4681 gctggctcgg ctttgtgctg atcaatgccg ttggggataa tggtcaccac tatcgcattc
     4741 gtcgtcaggg gtggctggaa cgagcggcaa aacaaacgcg attttatttg tttggcatta
     4801 tgcaggggga agaacatcct ttcccggcaa caacacagtc taaatttaat cccttacagc
     4861 aggtcgccta tgttggtgtc atgtatggat tgctgccgtt gttactattg acggggctgt
     4921 tgtgtctcta tccgcaagcc gtgggagatg tgtttcctgg cgtaagatac tggttattgc
     4981 aggcgcattt tgctctggca tttataagcc tcttttttat cttcggtcat ctttatcttt
     5041 gcaccacggg gcgtacgcca cacgaaacct ttaaaagcat ggtcgatggc tatcaccggc
     5101 actaacgaat gatcattaca cgagcagacc tgcgagaatg gcgtatcgga gcggtgatgt
     5161 atcgctggtt cctgcgccat ttcccacgcg gcggtagtta tgccgatatt catcatgcac
     5221 tgattgagga aggatatacg gactgggcgg agagtctggt tgagtatgcg tggaaaaaat
     5281 ggctggcgga tgaaaacttc gcgcatcagg aagtttcctc tatgcaaaaa ctggccacag
     5341 accccggaga gataccattt tgcagtcagt ttgcccgcag cgacgatcat gctcgcatcg
     5401 gctgttgtga ggataacgcc cgaatcgcaa ccgctgggta tgccgcgcaa attgccagta
     5461 tgggttatag cgtgcggatt ggaagtgtcg gctttaacag tcacattggc agctccggtg
     5521 aacgcgcact ggttgctgta accggtaatt cttcgcggat aagcagcgct ggagatagta
     5581 gtcggattgc gaataccgga atgcgagtac gcgtttgtac attgggtgaa cggtgtcatg
     5641 tcgccagtaa tggtgacctg gtgcaaatcg ccagctttgg cgcaaacgcc agaattgcca
     5701 acagcggcga caatgtacac atcattgcca gcggtgaaaa ttcaacggta gtgagcacgg
     5761 gcgtggtcga ctcaattatt ctcgggccag ggggaagcgc cgcactggca tatcatgacg
     5821 gcgaaagagt tcgcttcgcc gtcgccattg aaggtgaaaa caatattcga accggagtcc
     5881 gctatcgtct taatgaacag catcagtttg ttgagtgctg atagctttac ctttaatcaa
     5941 acgaaagacg ccgacgagca cgggatgcag gctttacgac cgcccgagcc atcgcctcac
     6001 caatttctgc acatgccgta agtccctgaa cgaaaggctg gtcgtgcatc aaccagggca
     6061 acgcgccgaa cgctacgcgt ccacgaatat cttcgggttg ctgtaacgta taatcttcgc
     6121 caacatcagg gatttcatcc cctgttttct gtaattgctc gcgtagccca gggaaaggaa
     6181 tatctttcac tttaagcgga cgctgtccgc gggcatcaat aaaaacgtca aacgagtagc
     6241 tgttgtcttc cgttttcagg acggtgcgcg actcattaat ttccattttg tagtcttcac
     6301 cgagggcgag aatatgaatg attcccgctt cacgtaaggc aagtaggcga cgaatagact
     6361 ctgaagggat tgccgcataa ttatcgatga aaaccctggc aaggccttta ctgaaccgtt
     6421 tatggtcgtg ttcattcaga tgaggaacaa tttcctgtac ggcttcatgc aggcgcagaa
     6481 tgacataacg ccagggaaca gtatgttttt ctcgtttatt gcgttcgact tcctggagat
     6541 ttttttctgc ccagtcaaat gggtcgcgtt gtttgcgctc ggcaaaccag gcttgagcaa
     6601 aggaatcgac attcaggctc tctaaggcta tgcgttggct ccagtctgga tcagcaaact
     6661 tgatttcctc tactatcaat ctaaataccc gatccaaaag gccctcttca cctttttgaa
     6721 tctcagcatt taatgcctga tcggtgacga tgtgtaaggg ctcgtaggga atagggcaat
     6781 agaaatcggc ttcgggtaaa atacccgtgc gcgacatcaa cgtaatattt agcttttcac
     6841 ttgcgttatc gcggttaaaa acgacgtgtt gtttatcatc ttcaatgaac gaaccatgct
     6901 gaatagccac tgccattgcc gcatccagtc cgctcaagga tgttcccata atacccacgt
     6961 tacacgcatc gacctttgct tccatcaggc ctgaccacgg actgggaaaa tacgttctgg
     7021 ttgcttcttc ttcatcaggc cagacgtgac ccgtggcaat caccgctaaa tcaaacgtct
     7081 cgctgggtaa atcctgattt gtagcgagca tgacgccagc atttgtaatt tgcagatcgg
     7141 taacctggca tgattcataa acagccactg caaatttttg ctgtcgtgcc tggtctacta
     7201 atcgtaaaaa ttgatcgcgg aaatattcgc ccagcagaat tcgcggtaaa aactgacgat
     7261 cgtgcaaggt ttctttttta acgccataac gctggagatg gctggcttct tgcttttgta
     7321 gccattcgag atacgtacaa ttaatcggcg gtatttcaat actggcaata tttgccagca
     7381 tcatttttga gttttcctca tcactgtatg gcatcccgac accggcctcg tcagcctgct
     7441 cgaagataga aatagaaagt ggagtttgtt gctgtagaag cgagaataag gtgtagatcc
     7501 ccgtaggccc ggcacccaca atagcaattt ttttcattgt tacttacctg tacgcattct
     7561 ttcgaattta gtgttatcag ataagtttgg ttgagatttt tagaatttga gaaatttcag
     7621 acagggatga aaagggcgct aaccgccctt gattaaattc tgtcaggcga gttttgcctg
     7681 attgatttga ctcagtggct cattgacatc gaaaactttg gcgacaactt cctcaacgcc
     7741 gagattttta agtcgggcag tatgtttttc cagataggca agggcgcttt tttcatcagt
     7801 aaaaagatag atcccaccgg cttcgtggtt cttttcactt tctgtccata ccttccacag
     7861 aaaaccaggt tcctgattaa tcgactcagc aagcggttta agctgctcag ccattgcgtc
     7921 gccaaatggg ccattaaaag caaaatgaag ttgtaacaaa gttgccatag cgattctcct
     7981 tctgataacg catacgaaga cagtgtaaat cttttaacgt attggataca gagaagaaac
     8041 agagggaaaa aggagaagac cagattaaga aaatctggtg cgtccgagtg gactcgaacc
     8101 accgaccccc accatgtcaa ggtggtgctc taaccaactg agctacggac gcaggatggt
     8161 gcgttcaatt ggactcgaac caacgacccc caccatgtca aggtggtgct ctaaccaact
     8221 gagctatgaa cgcaacgttg taggtgacaa cggggacgaa tattagcggc agagtgggaa
     8281 ggtggcaaga ggcaaaacgt aattttctgc gctatttcga ccgtttgcag agcttttaag
     8341 caaattggct atattttgtt gatttgcaag ggcgattttt attcaggatc gcatttacat
     8401 ctgatacaac ccaataattc gtgtatcaga gtttaaacag agttgtttgc caccatctca
     8461 aatctgtcgt ggataaatcc tccgtagcct ttacacttag gtagcaataa aggcacacag
     8521 atgcagcaaa ggaataaatc atgggatctg atgcgaaaaa cttgatgagc gacgggaatg
     8581 tgcaaattgt taagaccggc gaggtcattg gcgcgacgca acttactgaa ggcgagttga
     8641 ttgttgaagc tggcggaaga gccgaaaata ccgtggtcac gggggctggc tggttgaaag
     8701 tggcaaccgg tgggatcgcc aaatgcacac agtacggtaa caatggcacg ctatcggtca
     8761 gcgacggtgc cattgccaca gatattgttc agtccgaggg aggtgcaatt agtctctcta
     8821 cgctcgctac ggttaatggc cgccatcccg aaggtgaatt cagcgttgat cagggttatg
     8881 cctgcggttt gttgctggaa aatggcggta acctgcgtgt actggaagga catcgcgcgg
     8941 aaaaaatcat tctcgatcaa gagggcggcc tgttggttaa tgggacaacc tcagcggtcg
     9001 tggtagatga aggtggtgaa ttgttggtgt atccaggtgg ggaagccagc aattgtgaga
     9061 ttaatcaggg cggcgttttt atgctggccg ggaaagccag tgatacgttg cttgctggtg
     9121 gcaccatgaa taatctcggt ggtgaagact ctgacactat tgttgagaat ggatccattt
     9181 atcgtctggg gacggatggc cttcagctct acagttccgg taagacgcaa aacctgtccg
     9241 tgaatgtggg tggtcgggct gaagtgcatg ccggtacgct ggaaaatgcg gtaatacaag
     9301 gtggaacagt gatcctgttg tcacccacca gcgcggacga aaattttgtc gtagaggaag
     9361 atcgcgcacc ggttgaactg accgggagtg ttgcattact ggacggcgct tcaatgatta
     9421 ttggctatgg cgcagatctg caacaatcaa cgattactgt acagcagggc ggtgtgttga
     9481 ttctcgacgg cagtacggta aaaggtgacg gtgtcacttt tattgttggt aacatcaatc
     9541 tgaatggcgg aaaactgtgg ctgatcactg gtgcggcaac gcatgtgcaa ctgaaagtga
     9601 aacgcctgcg cggagaggga gcgatttgcc tgcaaaccag tgcgaaagaa atctcacctg
     9661 acttcatcaa tgtgaaaggg gaagttaccg gggatataca cgttgagata acagatgcca
     9721 gtcggcaaac tctgtgcaac gctctgaaat tacagccaga cgaagacggg attggcgcaa
     9781 cgctccagcc tgcgtaaaaa cacaggcgca acccgtgaag gttgcgcctt gtctttatta
     9841 gcgggatgct cgttgcagaa tgataactga cggcagacgt tgcaggaacc gcatacgcag
     9901 catcatcata atggctgccg acgtcaggcc aataataaag cctatccaga agcctgctgg
     9961 ccccataggt tcaacgacca gatcggtcag tgccagaata tagccgcttg gcaagcccag
    10021 cacccagtaa gccgtaaagg tgatatagaa aatggaacgc gtatctttat aaccacgcaa
    10081 aatcccactg ccaatcacct ggattgagtc agaaatctga tataccgccg ccagcaacat
    10141 caaatgcgca gccagcgtta caacctcggg attgtcgttg tacaacaggg cgatttgctc
    10201 ccgcagtgaa accgtgaata tggccgtcag ggttgccata cagacaccca ccataagccc
    10261 ggtccgcgca gcggtttgcg catccagcgt tgagccctga cccagacgat aacctacgcg
    10321 gatagttacc gctgccgcca gcgacattgg aagcacaaac atcagtgaac taaagttcag
    10381 ggcaatctgg tgtcctgcga catcaacaat accgagcgga gacactaaca gagccacgac
    10441 ggcaaacagt gtcacttcaa agaatagtgc cagcgcaatc ggcaaaccga gttgaatcag
    10501 tcgtttcata accgcaggat cgggtttcgc ggtgcctttt tcgttacgaa tatcgcgcat
    10561 ggagcgggcg cgtttaatgt aagaaaccat ggcaaggaac atgacccaat acaccgccgc
    10621 agtagccacg ccacaaccaa cgccaccgag ctcaggcata ccgaaatgac cataaataaa
    10681 gatatagttc accggaatgt tcaccagcag gccgataaag cccattacca tacccggctt
    10741 ggtttttgcc agaccttcac attggttacg ggcaacctgg aagaacagat agcccggcgc
    10801 gccccacaac aacgcacgca gataacccac ggctttgtcc gccagagccg gatcgatgtt
    10861 ttccatggag cggataatgt aacctgcatt ccacagcaca agcataatga gaacggaaac
    10921 aaaacctgcc agccagaaac cttgtcgcac ctgatgcgca atgcgctcac gtcgaccgga
    10981 accatttaat tgcgcgataa ccggcgttaa tgccagcagc agtccgtgac caaagaggat
    11041 cgccggaagc cagatagaag taccgatagc gaccgccgcc atgtcggtgg cactatagcc
    11101 gcccgccatc acggtatcga caaaacccat cgcagtttgg gcgatttgcg cgagaatcac
    11161 cgggattgct aatgctaata acagacgcgc ttcactgata tacttctgca cgtgaacacc
    11221 ttttatttgt agttatatga gagactaaaa agccgcctga atgggcagca aaaagaagaa
    11281 gcaggggaaa ttcagtctat tgtagcgagg atttattaat tctccagtga aaaaatagtc
    11341 attcttaccc cttcactggt aagggcgttt gccacctgct attgtgctga acagaatgtc
    11401 ttaactgatt tcaggagttg taagtatgtt tacggggatt gtacagggca ccgcaaaact
    11461 ggtgtcgatt gacgagaaac caaattttcg tacgcatgtg gtggagttac ccgaccacat
    11521 gctggacggc ctggaaacag gtgcttccgt ggcgcataac ggttgctgcc tgaccgtgac
    11581 ggaaattaac ggcaaccatg tcagttttga cctgatgaaa gaaacgttac gcattaccaa
    11641 tcttggcgat ttaaaagtgg gggattgggt aaacgttgag cgtgcggcga aattcagtga
    11701 tgaaattggc ggacacttaa tgtcaggtca tattatgacc accgctgaag tggcgaaaat
    11761 attaacctcg gaaaataatc gccagatctg gtttaaagtc caggatagtc agttgatgaa
    11821 atatatcctg tacaaaggat ttattggcat cgacggtatt agcctgaccg tcggcgaagt
    11881 cacgccaacg cgtttttgcg tccatttaat tccggaaact ctggaacgca cgactcttgg
    11941 gaagaaaaaa ctcggcgcac gcgtcaacat tgaaattgat ccacaaactc aggcagtggt
    12001 agatacggta gaacgtgtgc tggcggcacg agaaaatgcc atgaatcaac caggtacaga
    12061 agcctgatct gaaatcgccc ccggagaggg ggcgatagaa tagcctttag cgagcgactc
    12121 gaaggccgtt ttcaacaccg cgtgagaaca cgacctgcca gagctgaata tcacgggcgc
    12181 ggaaagcacc tgcacaggca ttcagataat aggtaaacat tcgtttaaag cgttcactgt
    12241 agttatccgc aatttctggc catgcggcga ggaatcgttc ataccacgcc atcaacgtag
    12301 tatcgtaatc agcaccgaag ttatgccagt cttccatcac gaagtggggt tcgctggatt
    12361 gagcaatctg gcgtacagag ggcaggcaac cgttcggaaa aatatattta ttaatccagg
    12421 gatcaacatt cagatcggtt tttttcgaac cgatagtatg gagcaggaat ataccttccg
    12481 gtttcaaatt acgatccacc accgcaaaat aggtatcgta atttttcggt ccgacgtgct
    12541 cgaacatccc cacagaaaca atacgatcaa actggtcgtt caggtcacga taatcttgca
    12601 gcaaaatggt gacatccagg ccttcacagc gttcctgagc cattttttgc tgttcggcag
    12661 aaatggtgac gccgaccacg cttacgtcat agttagatgc catgtagtgc gccagtccgc
    12721 cccagccgca gccaatatcc agtacgcgca tccctggttt taactgcaat ttttcacaaa
    12781 tcattttgag cttcgcctgc tgggcagatt ccagattatc ggcatctttc cagtaagcgc
    12841 aggaatattg catgaaggga tcaagcatgc ggctgaacaa gtcattaccc aaatcgtaat
    12901 gctccttgcc gactatccag gcacgttttt tactctgcag attgaagaga cgagcgctgg
    12961 caatacgcag cgtgtctttg aaatgatggg ggagttggtt ctcgagacct gcgcgtaaga
    13021 ctttgctaaa aaacatatcc agtcggtcac attcccacca gccatccata taactttcgc
    13081 ctaaccccaa agagccttct tgcagaacgc gtttaaaaaa atcggggttt ttcacacgaa
    13141 tatccgccgg ggcagaaccg ttaatggcta taccggcacg gctaagtaat tcgttggcga
    13201 tacggtacca gttgtcatcc ggtacactga cttcttctat acacgatgaa ctcatagttt
    13261 ctccatcact ggtcggtgat cagaacctta aaacagcgta gacgcttttt gggctttgtg
    13321 agaaatctcg cagaaaaaac cgcgagtcta taatccgacg tagaacagag ggaaaggagg
    13381 aacctttgcc gaaatgacca tccatagtga gtcgggaacg atcctgtccc cgtaaaaaca
    13441 taatatttat cgtagcttta acaactcgaa attagtatag gccgctaatt tcaatcatac
    13501 aacaaaattt tgttagcagc ctaagtataa gtatatcgat atagatcagt gtgattcgct
    13561 atgagcgact tcggcattgc catgattctg gcagccaact tcttcacaac gttgcatcat
    13621 gtaacccaga gcgaccagca ctactgttga taacatcacg ctggtggtgg tgagcaatgg
    13681 cgtgctgata ctgatcagcc aggaaactac cagacttgcg aggaagcaca gacccagttg
    13741 tagagtgttc tgcaacgctg cggcgcgacc agttgcgtgt gggaagggac gcagcgcctg
    13801 ggcgacaaca atcgggtaga tcgcgccatt ggcaatcgcc atcacacaga atgggatcag
    13861 gatttcgacc agcgacacat ggctaatgaa gcctgcagcc caggtcgcaa tgacgctgac
    13921 tgcaaacagc accagcaacc acggtaataa ctgcttgcct tgccatttct gcagcgcggc
    13981 gcgacagcca taaccaccaa tcagaaacgc gatagtttgc gggacataac ttaaaccaat
    14041 aactgccggg ctgtagccca tttcactaag gatgaacggt gaaccggtca gccatgcaaa
    14101 aaaactggct gaacaggccg cgtatatcag cacgttgccg cgataggttt tagaacgtag
    14161 caggtcggta aaggtcagac catcctgact attgttacgg gccttcgtcg tgggtttgag
    14221 ccagaaaata ggcagaatca gcaccacggt aatggcaaac agggtggcga aaatcgcctg
    14281 ccaggaaaaa tggaccagca gccagcttcc taacagagga gccagggccg gagatagacc
    14341 caccagcggc atgatggtcg caaaaattcg gttaactttc tgtgaaggat aataatccgt
    14401 cactaacgct tgccagataa ccgccgcggc gcagacaccc acagcctgta caaaacgcaa
    14461 taccagcagc gtagcggcgt tttctaccca cagcatcccc agactaccta acgcaaaaat
    14521 tgtcaggccg attaataaca ccggtttacg accataacgg tcggagagtg gcccccacag
    14581 aagctgggct gcagcaaaac cggcaaggaa cagactaagg ctggcactga cagcagacgc
    14641 aggcgtttgc aggtcggcct gtatggcggc gaaagcaggc agatacatat cggttgccag
    14701 aaaaccgagt acgctcaaac ccgccagcca gactaaaaat cttttcccag gttgcatgta
    14761 tattctctaa atctctcaat taaacaccgc cgcaaagtgt agggagtgaa attagtgttg
    14821 tgaaacggta atatttgatg actggtttca aaaattttgc aggtagacta tgtggtcaga
    14881 atattcactc gaagttgttg atgcggtagc gcgtaatggt agttttagcg ctgcggcaca
    14941 ggagctgcat cgcgttcctt ctgcggtcag ctataccgtg cgtcagctgg aagagtggct
    15001 ggcggtgccg ctctttgaac gacggcaccg tgatgtggag ctgaccgctg ctggagcgtg
    15061 gtttctcaaa gaagggcgct ctgttgtcaa aaaaatgcag atcacccgcc agcaatgtca
    15121 gcagatagcg aacggctggc gcggtcagtt agctatcgca gtggataata ttgtcaggcc
    15181 agaacgtaca cggcagatga tcgttgattt ttatcgccat tttgatgatg tcgaacttct
    15241 tgtctttcag gaagtgttca acggtgtctg ggatgcgctt tccgacgggc gcgtggaact
    15301 ggcgattggc gcaacacgcg cgattccggt aggcggtcgt tatgccttcc gggatatggg
    15361 gatgctaagc tggagttgcg ttgttgccag ccaccacccg ctggcgttga tggatggccc
    15421 attcagcgat gatacgttgc gcaactggcc gtcgttggtg cgcgaagaca cctcgcgaac
    15481 gctacctaaa cgtattacct ggttgctgga taaccaaaaa agagttgtcg tgcctgactg
    15541 ggaatcatcg gcaacctgta tctcggcagg attatgcata gggatggtgc caacacattt
    15601 cgccaaaccg tggcttaatg aggggaagtg ggtagcactt gaattggaaa accccttccc
    15661 ggattcggca tgttgtctga catggcagca aaatgatatg tcgcctgcgt taacctggct
    15721 gcttgagtat ctcggcgata gtgagacgct gaataaggag tggctgcggg agccggaaga
    15781 gactcccgca acgggtgatt aacgacgata gtcgcggaac gggccgtcag ccacggagcg
    15841 gcgttcaatc aagcgcggat gcacttcaat ggactgcggt tcttcacgtt tgttgacgat
    15901 acgatccaac agcatgttga acgctgtttc acccagcgaa tcttttggct ggtggatcgt
    15961 ggtcagcgcc ggcgtaaaat agcgggcgtt gcgcacgtta tcataaccga tcagcgaaac
    16021 atcctgcggg acgcgcagac ccatttcatc agcagcacaa agtgcgccca ttgccatgat
    16081 atcgccacca cagaagacgg cagtagggcg atgcggctgc gacagtattt gctgcatggc
    16141 gcgataacca gattcgggtt caaagtcacc ctgaacaatc cagctttccg gcaccttgat
    16201 catcgcttct tccatcgcct tcataaaacc ggcaaggcgg cctgcgccgg tgttacgttc
    16261 cagcgggccg gggatgacgc cgatttcgcg gtgaccgcgt tcaatcagat aacgcccggc
    16321 catgtagccg ccttcgaacg cgttatcaat gaccgcatcg gtgaagtcag cttttgcttc
    16381 accccagtcc atcaccacca ttgggatatg gcgatactct tccagcatcg ccagcaacgg
    16441 ctctgggtac tcagaacaca tcaccagcag accatcgacg cgtttttgcg ccatcatcga
    16501 cagataagcc cgctgtttct caagattgtt ccacgcatta cccagaatca gggtgtaacc
    16561 tttctggaag caattttttt caaccgcttc aatgatctcg gcaaaatagg ccgcttcgct
    16621 gctggtcgcc agcaaaccga tagacttggt gtggttaacc ttcaggctac gcgccaccgc
    16681 gctaggggag tagtgtaatt ctttaatcgc tgcccacacg gcgttgcgcg tttcttcagc
    16741 gacgaaacgt gttttgttga ttacgtgtga cacagttgta gtggaaacgt ttgctcgttt
    16801 cgctacatct tttattgttg ccattccatt tcactccaga ccctaacttc agctcctgca
    16861 aaatcgcaag gtaaacgttt gcctttacac accttacgca caacaaaagt gttgcggtac
    16921 gccggaaaaa cgacacggag cgtcaggaag gggtcaatgg ccagtacgca aatagtgtaa
    16981 gcgtggaatt ttggccgatc ttgacgaaaa ggggaagtgg taaaaacact tatcattata
    17041 aatgacggga gattttttca tcacagtgtg taaaaatgcg taatatcact ttttgtgtgg
    17101 taataaaaaa ggagaaaact tgatgagcac cgaccttaaa ttttcactgg taacaacgat
    17161 tatcgtcctc ggtttgatcg tagccgtggg tttgactgcc gcgctgcact gatttcctct
    17221 cgagggagcg cgttctccct cgcatctcct tcctgtttgt tagcaaaatg gcaaaagtgt
    17281 tttctctttt tgttattttc atttttttgt gattgctgtc atgctttcgc cactttaatg
    17341 aatatcgcgt aatgacgcga agaggtggag ataaggcatg aaaattaact atccgttgct
    17401 ggcgctggcg attggcgcgt ttggtatcgg gacaacggag ttctcgccaa tgggcttgtt
    17461 gcccgtcatt gcgcgcggtg tggatgtctc gattcccgct gccggaatgt taatcagtgc
    17521 ctatgcagtt ggcgtaatgg tgggcgcgcc gctgatgacg cttctacttt ctcatcgtgc
    17581 ccgccgcagt gcgttgattt tcctgatggc aattttcacg ctcggcaacg tactttccgc
    17641 catcgcgccg gattatatga ccctgatgct ttcacgcatt ttgaccagcc tgaatcacgg
    17701 agcatttttt ggtttgggtt cagtcgtggc cgcaagcgtg gtgccaaaac ataaacaggc
    17761 cagcgcagtt gccactatgt ttatggggtt aaccctggca aatatcggtg gcgtgccggc
    17821 ggcgacctgg ttgggtgaaa ccatcggctg gcggatgtca tttctggcaa cggcggggct
    17881 gggagtgatt tcaatggtaa gtctgttctt ctcattacct aaaggtggtg caggggcacg
    17941 acctgaagtg aaaaaagagc tggcggtatt aatgcgtccg caggtgctgt ctgcattgct
    18001 gacgacggta ctgggagctg gtgcaatgtt tactctctac acctatatct ctccggtact
    18061 gcaaagtatt acccacgcaa caccggtgtt cgtcacggca atgctggtgc tgattggtgt
    18121 cggattctct atcggtaact atctcggcgg caaactggca gatcgttcag ttaacggcac
    18181 gttgaaaggc tttttgttgt tgttgatggt gattatgctg gcaatcccgt tcctggcccg
    18241 caatgagttc ggcgcagcta ttagcatggt ggtgtggggc gcagcaacct ttgcggtcgt
    18301 accgccgtta cagatgcgcg tgatgcgtgt cgccagtgaa gcgccaggtc tgtcttcatc
    18361 agtcaatatt ggtgccttta atcttggaaa tgcgctggga gcagctgctg gtggtgcggt
    18421 aatttccgct gggctgggat acagctttgt gccggtgatg ggagcgattg tcgcgggact
    18481 ggcattattg ctggtgttta tgtcagccag aaaacaacct gaaacagttt gcgttgctaa
    18541 cagctaatta aacaaaaacg cagaaggata accttctgcg ttacagttgc tgacaacgtg
    18601 cgatttatct tgccgaatgc gctgttaacg cctcatccgc gttggcatgg taagcgaagt
    18661 gcatcaggca atgctgcatt tgccatcagt tattatgcag cgagattttt cgctacgaat
    18721 tcccagttca ccagcgccca gaagtgctcc agatagccag gacgtgcatt gcgatagtcg
    18781 atgtaataag cgtgttccca gacatcaacg gtcagcagcg gagtcgcatc ggtggtcagc
    18841 ggagtacccg cgttagaggt tgaaacgata gccagtttgc catcgctgtt tttcaccagc
    18901 caggtccagc cagaaccaaa gtttttgatc gctgcatcag taaactgcgc tttgaaatcg
    18961 gcaaagctgc caaaagatgc ggcgatagct tcagcgactt ttccagtcgg ttcgccaccg
    19021 gcgttcggtg ccaggcagtt ccagtagaaa gtatggttcc agacctgagc tgcgttgttg
    19081 aatacgccac cttcagagct gcgaataatc tcttccagtg atttaccttc aaacgcggta
    19141 cctttaatca ggttgttcag gttagtgaca taagtctgat gatgcttgcc gtagtgatac
    19201 tcgatagttt ccgcagaaat gtgcggtgcc agagcatctt tagcatatgg tagtgcaggt
    19261 aattcgaatg acattgctac tctcctttat tattaatttg catacgtaca atagccttat
    19321 tgtgcgtatg gatagggtag caaattaaaa ggtgagacaa aagatgaact taccctgttg
    19381 ccgtaacaac agggtaaagt tttagcgaag tgtttttggg gtcattaccc gacgagcgcc
    19441 aacatagtgg cgctgccagt agtcttcact gagagaagtg atttgaattt cctgacctgt
    19501 gcgcggtgac tgaataaatt tgccgttgcc gacatacacg ccgacatgat cggctgtgcc
    19561 gcgtccctga gtacggaaaa agaccaggtc gccgtttttc agttcactac gttcgattgg
    19621 cgctgcatca cgcaggtgat acatttcatt cgccgtacgc ggaatacgaa ttttcaccaa
    19681 atctttataa gcgtaataaa ccaggccgct gcaatcaaaa ccggtacgcg gtgagctgcc
    19741 accccaacga tatggcttac caatttgctg catcagttta ttcattgcca ctttcgtagc
    19801 tttttgcacc ttcgctttgt gcgcgtcggc aagagttcct gacgcggcat ttttgacttt
    19861 cacacaatgc gatttatagc ccttacgttt ggtacacttt tcggtaaagc tggctgaggc
    19921 cgttttattg acggcgtgtt tgctgcgagt tttggcgttt ttagaagcgg taatggaaga
    19981 ttttttaacg gaagaagcgg ttttgctttt ggtcgtactt ttactggcgg cttttttcgc
    20041 tgttttctgg gtctttttgg tggttgccgt gcttttcttt ttatctgctt ttttggtgat
    20101 atgagtggta gcagaactct ccctggcttg ctttgaagca tgggccatag gcgttaaagg
    20161 cagggtggta aaaagtaaag cacagagcgt gatcgaaata cggtttatcc gcgccactga
    20221 gcgtccctct gttaaggcag gtctatcatg cctgcgtctg atttacaaaa cttgatgatt
    20281 ctaatgcata gaagaagaga aagttaatag cttttattgt taaaaaatac gtttcgttgg
    20341 cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtt ataaatacgc cagtttgccg
    20401 cgcatcagag aagaaacgaa tatttgaaat gcaacaaata gagcaaaggg ataaaatagc
    20461 aaaagcgtga tgaaaatgtt attttccgat gccgcactga cccgctacaa tgtcagatta
    20521 ctgccgtaaa gaagtttgag gaagcaagac aatgagcacc actatcgaaa aaatccaacg
    20581 ccagattgct gaaaacccga tcctgctgta catgaaaggt tcaccgaaac tgccgagctg
    20641 cggtttctct gcccaggcag tccaggcgct tgccgcatgt ggcgaacgtt ttgcctatgt
    20701 tgatattctg cagaatccgg acattcgtgc ggaactgccg aaatatgcta actggccgac
    20761 cttcccgcaa ctgtgggttg acggcgagct ggtcggcggt tgtgatatcg tgatcgaaat
    20821 gtatcagcgt ggcgaactgc agcagctgat caaagaaact gccgctaaat acaagtctga
    20881 agagccggac gcggaataat ccgtcctgca atactgacgg atgcgaacgg gcatccgtca
    20941 gtcctttctc tatccccaat ccagcccttg tggcgaactt gaaaatcccg cctcacgcag
    21001 cagcgtaaac atcggcgttt gccggaccgg tagatcattc acttctgtta gcgtaaagcg
    21061 cagccgtggt tcgcgacgca gtgcggtagt cagcgcgtgg aaaacctctg gggcgagtaa
    21121 ttcctctttt tcctgccaga ccagcatttt tttgccacct tgcgccagat agagtaacaa
    21181 tttgccgcca gaaacgacca ccagcgcacc cgcccgacgc gttggaacca gcgttgccgg
    21241 atgtgcaggc cagggcagaa gatttcccca cacattagcc ggatcgttgg cagagagcgc
    21301 cactggtgta tagtggcgcg tttgcgtggc ttgtgtcgcc agatcgcgca atcggtcaat
    21361 agtcagacgt tcagcgaatt gcgcgccacc cagaccttct acaaaacgac ctcgcataat
    21421 tcgcccggag tcttccatac ttcgacaaag cgtttgcatc gatggaaacc cgccagggat
    21481 attttcggct atcaccgcct gacgactgat gatgccgtag cggtcgagca tattttccgc
    21541 cagcgccagc atcctttcgg tatcgtttag tggctccacc tgcaataacg accagcgtcc
    21601 agccaaattt ggtgtgttgt aagataccag cggcgagacg ggttgcgcat agacaggacg
    21661 tccacgacga gcccggtgac tgcggcgagt tgaggtgcgt gcgttggaac tgctgcgggt
    21721 gagggcgcgt aacggtgccc aaatgtcgct ggtgatgacg ccttgccaga ccagcgccca
    21781 taacgcctct tgcagggcag agagatcaac cgattcgccg attttgtcgc gtatccgttg
    21841 gctgatttgt tgtgcaaacc aggctcctcc gtcagccaga acagcgacta tcgcttgttg
    21901 cagcgccgaa cgattcgcct gatccgcttc ggcgggagtg aacgattctg cagcatattc
    21961 ctgtagatgc agtgccacca ggccgtcatc ttcacccagt tttttttgcc ccgaccagat
    22021 aactgcaccg gttgccagta attcatcgag catttctgac gaatagtcgc gtacgcgggc
    22081 aggcaggatc tggctttccc agagtgaggc gggtaaaccg actccggcaa gctgttcgat
    22141 cacccgcatc acgccatcta cgccctcata aacgcctggc gatgttgagg caaagagcgc
    22201 cgggctacca tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt
    22261 cgtggctgca acgggacgcg tcgcttctct ggcggcttgc agcgagcgca aacgcagacg
    22321 acgaaatact tcatcgctca cccagatgtc ttgttgcaga ttcatcacca gaccctgttc
    22381 acgcagttgc tgaagctgct cttcgacaat ggcaataccg agactaaact catgagccag
    22441 ttgttcagcc gtgaccagag catgagcccg gagatagcgc agaaagaggt cgcgaagcgg
    22501 gtaactgact ctatgtaaat aaatctctgg caatgactct ggtagtcgta cgccgagggc
    22561 atcacgcagc ctggcggcat catccataca ggcaagacgc tcctgtccgc taatcatcgc
    22621 tgggaagatt cgttttactg caagaagatt ttccagatac gacgcaacct cttcactgct
    22681 gcctgtatgc cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc
    22741 gaacaatcct tcttcacctt tcgctcttct gccaggagcc agtcgttgca actcttcttc
    22801 cacctggcga atgacctgcg ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt
    22861 gcgcagtaac tcgctgtcca gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc
    22921 gctctggtac ataaattccg cgacatagcc gaacaataaa cttgtggcga aaggcgaggg
    22981 cgtagtggtc gttacatcgg atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg
    23041 ttccagtgcg ggaagatcat aaacatcttg cagacattcg cgcagggttt cgagaatgac
    23101 cggaaaatcc ggatatccct gagcgatttc cagcaactga ctggcgcgca gccgttgttg
    23161 ccaaagcggg gtgcgatggc ccggagtgcg ccccggcatt aataatgccc gcgcggcgca
    23221 ttcgcgaaaa cgggcggcga aaagtgccga gctgcctacc gcctcgcgga caatttgcag
    23281 caacttttct ggttcaaaca aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg
    23341 aatgcgcgca acaatgccgt catcactggc gaccacagac gcgtcagcgc cccatagggc
    23401 atgtattcgc ccggtaatcg ccagcgccca gggttcatgc acccgtcttc catagggaga
    23461 gtgcaaaata atacgccagt cgccaatttc atcacggcac cgttcgagga ccagatggcg
    23521 actccccgga acgatgcccg tcgcgttgcg ttgctcgtca atcaacccct gaatattggc
    23581 gttcgtattt tcttctgcca gccacggggg aatagtcccg gaaaagaacg cgccatccgc
    23641 cagcaaatga agaaaatcgc cgatcatctc gcctaattca gccggacgtc cgttaccttc
    23701 accacgccag aaggggagcc gggcagaacg acccggagca ggagtcacaa tcacctgatc
    23761 gcgggtgatt tgctggatcc gccatgaggt agcgccgaga gtgataatgt cgttcacccg
    23821 cgactcatat accatctcct catccagttc acccacccgc cgcgaaccgg ccttttcttc
    23881 accttcgggt aataacacgc tatacatgcc acgatccgga atggtgccgc cgctggtaac
    23941 cgccaacaat tgagcgccag gtcgggcggt caatatcccg gtctccctgt tccagaccag
    24001 tttggggcga aaagcagaaa aatcgccaga gggatagcgc ccggaaagca tatccagcgt
    24061 ggcgtcaaaa acacgtcttg gcagatcttt ccacggtgcg gcacggcgta cgcgggagta
    24121 ccattcgtct acctgtaatg cctccatcgc cgcggcggca acggtttgct gcgcaaggac
    24181 gtcgagtgga ttatgcggcg gtgtcaggtt ttccagcctg cctgcgaaca tacactctac
    24241 aatgactgcg gaatcgacta agtcacgccg ggtacgaggg aaaaacagcc ctttagatac
    24301 accgccaacc tgatgtccgg cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg
    24361 cgttgccacc tgaatcacca gatccactgc gcccatatca atccccagtt cgagactgga
    24421 ggttgcgacc acgcagcgta actcacccga tttcagcgcc tgctcggtga ttgctcgttg
    24481 ttctttagag acggagccgt ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg
    24541 gttagaggtt gcgccggagg tcgactcgaa atgggccgca tcaacggcga tagacgggga
    24601 acgctgtaag cgtgcggcgt aaagctcatt taatcgtgcc gtcagttttt ccgccagccc
    24661 acgtgaatta gtaaagacaa tggtcgagcg atggcgcaac acttcatcaa ggatacccgt
    24721 ttcaatatat ggccagatgg agccttcccg gccggcatgg ctgtcttcgc cggtgctgct
    24781 ggcgaccgat gagacatcat ccatattggc gaccggtacg acaattcgta tctgcggatg
    24841 gcgcattgcg ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac
    24901 atcgctggct gagcgcacag tggcagaaag gccaattcgc tgtgctgagg tgtggagcag
    24961 cgcatcgagc cgctccagac ttaacgccag atgcgcacca cgtttactgc ccgctaccgc
    25021 gtggacttca tcaataatta ccgtttcgac gccgcgtagc gtttcgcgcg cgcgggaggt
    25081 cagcatcaga tagagtgatt cgggtgtggt gatcagaata tccggcggat tacgggtgag
    25141 tttgctgcgt tcctgtgcag gcgtatcgcc agtacggatc cctacgcgaa gattgacttc
    25201 cgtttcgccg cgccgccgcc gttcatcggc aatacccttc aacgggagct gcaagttgcg
    25261 ctgaacgtcg gtgcccagag cttttatcgg tgaaatatag aggatgcgtg aggttttacg
    25321 cttatgcgcc tcgcgggtat cttcgccgcc ttcgcggaag agccgatcga gggcgtagag
    25381 aaatgctgcc agcgttttcc cggagccggt cggtgcaatc accagcgcat gttcgcttcg
    25441 cgccgccaca tgccaggttt gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa
    25501 ccagtcgcgg gtcgccggtg aaaacacgtc cggcaggagc gatgaagggt ctggattatc
    25561 tgccattaac gctattcccc gtgaatatca tgcaggatgc gtggaatagc ctcactatag
    25621 atgcctgaat cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcaggt
    25681 agcggccagc ctcccagacg tttccagcgg ttgacgattt cacaaaacag cacagcagtg
    25741 cgttcggtgt cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc
    25801 tggcaagcct ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca
    25861 aaagtggcga aagggtggaa cgggttacgt ttcagtgagg cgcgttctgc ggcggccatc
    25921 ataaagctgt gatcaaaatt ggcattgtgc gccaccataa tggcgcggtt acagccgctc
    25981 gctttaatac ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc
    26041 gcgccgcgat cgggatcgtt cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa
    26101 tttgcgccga caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct
    26161 tgttcatcca ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta
    26221 aatccggctg tttcaacatc gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga
    26281 ccggtaagtt gagcgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc
    26341 attatgacaa atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg
    26401 gcaggagatt agttgcccag accgcgaccg gcgtctttct cttcgattaa ctcaattttg
    26461 taaccgtccg gatcttccac aaacgcgata accgtagtac cgccttttac cggacccgct
    26521 tcacgggtca cgttaccccc gttttgacgg attttttcgc acgcttcagc ggcgttatct
    26581 acgctaagcg cgatgtgacc ataagcagtg ccgagttcgt atttatccac gccccagttg
    26641 taggtcagtt caatcaccgc ttcttcggtt tccgggccgt agccaacaaa cgccagtgag
    26701 tatttgtatt ccgggttttc gctggtacgc agcagtttca tgcccagcac tttggtataa
    26761 aaatcgatgg agcgttgcag atcgccaacg cgcagcatgg tatgaagaag acgcatcttt
    26821 ttatcctcaa aatggctaac agattcaaaa tgttgtttta gtatagcggc ggctttacgc
    26881 cgctcgcaat gttggattac aacgtcgggt aatcggtata gccttccgcg ccgccaccgt
    26941 agaaactttc ggcacgctgt gggttaagct cagctttgcg ctgcaagcgg gcgaccagat
    27001 ccgggttcgc aatccagtca cgaccaaatg ccaccgcatc aattaaccct ttgccgatca
    27061 gcgtttcagc tttttctact gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg
    27121 cgcgtacttt ttcgcggaac gcatcagtat acggttcacc ccccgcccaa tctggttctg
    27181 acatatgcag ataagcaatg ccgcgtttac ccagttgttc aatcagatac agtgcatcgg
    27241 cttcttcatt cgggccgtta tccgtgttct ggaaagtacc gattggcgaa atgcgaatgc
    27301 caatgcgatc ggcaccccat tcttcaatcc cggcatcgac cacttccagt accagacgtg
    27361 cgcgattttc cacgctgccg ccgtactgat cggtacgatg gtttgaagaa ggagaaagga
    27421 actgatgcag caaataaccg tgagcagagt ggagctctac cagatcaaaa ccggcttcac
    27481 gcgcgttagc aatggcctga cggaaatcat tgacgatacc tggaatctct tccagttcaa
    27541 gcgcacgcgg catggatgtt tcaacacgga tcgcctgacc attttcatcg cgcagagaag
    27601 tacgtgttcc cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc
    27661 tggcgtgaga aatgcgtccg gtgtgccaca gctgcacggc catatgacca ttttcagcat
    27721 gaacgccagc ggtgattttt ttccatgcgg caatttgctc cggactatgg atgccaggcg
    27781 cacctgcata tccttttgcc tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg
    27841 cactggcacg ttggcgatag tattccgcca tcaacggggt aggaatgtca cccggttcaa
    27901 tactgcgcag acgcgtcagc ggtgccataa aaatacggtt tgccgccgtg atcgcgccca
    27961 ctttcagtgg ggaatacagt ttttcagatg acataaaggc tcccggtcgt ctggtgataa
    28021 aaaatgcctg ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt
    28081 ccagtggctc gaaactgcgc gaaattttgg cctgcagatt cgcacccagc catagtgcgt
    28141 aaagcacttg tgcctgttgt agcggttcgc cacaaaaggt taaacaatgg ttctcacggc
    28201 cattttccag cgcctgagag agcaggtcga tcacgccgcg agcaccttta tccatcgcgc
    28261 tgcgcatatc ttctgacaga tcgcacactt cggcagagag ttttactgtc aggcaaccac
    28321 tgatggttcc atgttggcaa aactggttca gtgtttgctg gtaataagcc agtatgcggt
    28381 cgcggtagtt accttcgccg gattgcagca actcagtcag tcgctggtga tatgcggcgt
    28441 aatgacgctc aagcatggca acgccaaacg cttctttaga gcgaaagtag tgatagaagg
    28501 accctttcgg cacttcagcg gtttttagta attcgcttag ccccatcccg gtgaatccac
    28561 gttgcaggca aagttgctcg cccgtcgcca ggagatgttc gcgagtatca tgttcggtgt
    28621 gtttgttcat gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa agttgctact
    28681 ttggacatta acagcttcaa taatggtaat aatcttatct gaggagggcg aagtggcgga
    28741 gcaattagag ttctttcccg tccagagccc gtgccgggga atttgccagt ctgacgaacg
    28801 cgggttttgt cgcggctgtt ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag
    28861 cgatggcgaa aaacaagagg tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg
    28921 cgcaaataag ccagcgtcat cagacgaacc ggagcaacca tcactctttt gaacctaatc
    28981 ttgcgtatac tcctctcata tctgataaga ggaagcgatt atggttcagc gtattactat
    29041 tgcgccgcaa ggcccggagt tttcccgttt tgtgatgggc tactggcgat tgatggactg
    29101 gaatatgtcc gcccgccagc tggtcagttt tattgaagag catctggatc tcggcgtgac
    29161 caccgtggac catgctgata tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc
    29221 actgaaactg gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc
    29281 gacgaccgcg cgtgaagaaa acgtcattgg tcattacatc actgaccgcg atcacatcat
    29341 taagagcgcc gaacagtcgc taattaatct cgcgaccgat catctggatc tgctgttaat
    29401 ccaccgacca gacccgttaa tggatgccga tgaagtggcg gacgcgttca aacatctgca
    29461 tcagagcggc aaagtgcgtc attttggcgt atcgaacttt acgcctgcgc aatttgccct
    29521 gttgcaatca cgtctgccgt ttacccttgc cactaatcag gtggaaatat ccccggtgca
    29581 tcagccgtta ctgctggatg gcacgctcga ccaactacaa caactgcgtg ttcgtccgat
    29641 ggcgtggtcc tgccttggtg gtggtcgtct gtttaatgat gattatttcc agcagctgcg
    29701 tgatgaactg gctgtggtgg cagaggagtt aaacgcgggc tcgattgaac aggtggttta
    29761 cgcctgggta ttacgtttac catcgcagcc gctgccaatt atcggttcag gtaaaattga
    29821 gcgcgtacgg gcagctgtcg aagcagaaac actgaaaatg acccgtcaac aatggtttcg
    29881 tatccgtaaa gcggcactgg ggtacgacgt accgtaagca cttttaggaa tagccgccgt
    29941 tcaaaaatgt gtcactggtt tacacttatt caggaatgca caatgaacgg aggtcctatg
    30001 aaacgtttta gtctggctat tctggcgctg gttgttgcaa ccggcgcaca agctgccagt
    30061 gaaaaagtcg agatgaacct cgtcacgtcg caaggggtag ggcagtcaat tggtagcgtc
    30121 accattactg aaaccgataa aggtctggag ttttcgcccg atctgaaagc attacccccc
    30181 ggtgaacatg gcttccatat tcatgccaaa ggaagctgcc agccagccac caaagatggc
    30241 aaagccagcg ccgcggaatc cgcaggcggg catcttgatc cacaaaatac cggtaaacat
    30301 gaagggccag aaggtgccgg gcatttaggc gatctgcctg cactggtcgt caataatgac
    30361 ggcaaagcta ccgatgccgt catcgcgcct cgtctgaaat cactggatga aatcaaagac
    30421 aaagcgctga tggtccacgt tggcggcgat aatatgtccg atcaacctaa accgctgggc
    30481 ggtggcggtg aacgctatgc ctgtggtgta attaagtaat tacgaggcca gcgtaccttg
    30541 cggtggtgct tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa ttgccgccag
    30601 ctcacgggcg gcaggttgat gatgacgggc aaggctgtcg caaatccgct gtaattcttc
    30661 aagtgtggcc gccagtgatt tttgctgaac gccacgctca ctcatcactc cccgcaacag
    30721 tgaaatacag ttatcccgta ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa
    30781 ttgccagaca acatgagaac agttcagcag cactacaccc cagcgtaata accaacggcg
    30841 agccagcgca tcctggctgt tactcaactg actgacgtga tgataagtga gcgattcaaa
    30901 ttcgctttca ctcagtgttg gatggcggct tagctgatcg acaaaatccc ggcgcagcgc
    30961 gcgaatatgg cggcggcttt tacgagcatc cgatcctgga cgcagaatgg cgaacgctag
    31021 ccaagccagc gcaacgccaa cgatttttgc cagattatcg ttaagaaaat cagcaaaatc
    31081 atacaccggg ggattagtga cagcgataaa cgaacccata aaaacaatca gttgccccca
    31141 cagtgcggca aatttgggca tctgcaattt aagaagctgc attgtcgcca gcagtggaaa
    31201 gagaaacagt aaaaattgcc acagatcgct aatctggacc atcaggccaa atttgaccac
    31261 aaagctgaat agcgaaagta acaccagcgt gcgcatcagc agtgacaacg acttaaacgg
    31321 tgctgcgacg gcggagtaga gtacgcagct aattgctgcc agcgttaatg cattggcacc
    31381 ggcatcccat tgcgatgcaa tactccatgc gccaatcatc atcaacgtac aaaatgtacg
    31441 cagcccgctc cacatagctt cggcgttgtc ggtatgacga gccagagcgg atgtccgtgc
    31501 gagtctggca tgatcatcta caccgctttg cagacgatgt aattcctgac tactttgcag
    31561 ataaaggcgg caaaaataac gtagtcgctg ccagaaggcg acgtgccggt agtcggcgac
    31621 gttggtcggg cgtagcgggg cgataatacg tgcgacggtg taagcatctg tttgcgaact
    31681 ggcgagcgcc gtcagcaatt gttcgagaat ttctcgcgtg gcacctggcg gtgagggcca
    31741 gttgagcaac atacgtcgca ggctggagat gacactggtc atacgtaatt gctggtggag
    31801 tagcgcatta aggcgcgcgt tttgctggcg aaaacgatag tggctccaga aagcctggat
    31861 acgcagcaaa ttcatggtca gtatctgccc aatcaccccc tcatgtgctg cacgaatggc
    31921 atcgtttgtt tcaggctgcc agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt
    31981 tttcaatgcg gttaaaaggg ctgtagcatc ggaactgcta ggcaggatca tcatcatcat
    32041 tccgccgcac aaaataccga ctatcacctc gcaaacgcgc gcctgagcga tatcccacag
    32101 ctggctggcc tcagtaatat taaccatcgg aaaggcgatg atggcagccg tgtagcccgc
    32161 cagttgaaat gcatacgcga cgttattcgt gaagtgcgca caggcccagg tacaaaagcc
    32221 aagccacgcc gacatgctca atagaaaaaa ccacggctca ttgagcgtat gcccggcaag
    32281 aagcagtgcc gcaatggctc ccagtaaact gccagcgatg cgtccgaggc ttttgctgat
    32341 gacaccgcca acggtgggaa agctaaccac tgcagccgag gtcatcgccc aatagggttc
    32401 atccagattc aaataatagg caaccgtcag cgccagacac atggcaatgg tattgcgtaa
    32461 cgcataacgc cattgcgcca gcgtggcctt gaaccagggc aaattgcgca aggaccatga
    32521 cgatgcgttc attattgacc tacggcaatg ctacaggtgg tgcccgacac cagcgtgaca
    32581 tcgcctggaa ctttatccag cgcaaagcgg acgggaacgc gttgggcgag acgaacccag
    32641 gggacgttag gtttcacatc cggaataagg cttgagtcac tttcaacgct ttgatcataa
    32701 atcgcccgac cgatactgga aacgtgaccc cgtaacgttt tgttatcact atagagggta
    32761 atttgtgctg gtgcaccttc gcggatatgg cgcaacttgg tttcttcaaa ataaccaatg
    32821 acgtaaaacg agtggctgtc gacaagggca aacagcggtt tcccggtgtc agcgtaatcg
    32881 ccgatgcggg tagtgaggtt agtcacccaa ccactcaccg gggcgcgaat ttccgtttgc
    32941 gccagttgcc attgtgtctg tttcagcgtg gcctgtgcgg cattaacact ggcctgcatc
    33001 gctttaacat tgaggttagc ggtatcgagt tcttcggcag aaataaaatt ttgtgagaga
    33061 tgacgacgac gattcgcttc gttattggct tttgcgaggt cagattgcgc tttcgccagc
    33121 tgagcctgtg cgttcagctc ggcgatctga aagggcgttt tatcgatcgt gagcagaaga
    33181 tcccccgcgt taaccagttg gttgtctttt atattcagct caacaatacg tcccgacacc
    33241 tgtggggtga tagaaacctt ttctgcgcgt attttcccgt cgcgtgtcca cggagattgc
    33301 atgtaataat tccacagcca ccatccggca agaaccgcaa ctatcgctac aatgattgtt
    33361 gagaaatact taattgtttt aattgacata gttaccacgc aatcagtatt gccagagcaa
    33421 ggcaaacgca aatcgcaaac agcgataaat ccattaacaa gggatgccag atgtcaccgg
    33481 cgtagatcca gccacgaagc aggcggtgta cgacaagcca gatgacgaat ccaaacgcga
    33541 aggctttgaa aaacggagga aaatagacgg acgcaccgaa caccagatct tgtaagggca
    33601 atcctgttgc attgagcata aacttcacaa agtagattcc tttacgaccg aatatattgc
    33661 gttagattaa taaatattct ttaagtgcga aaaatttacg cgcaatttct gaaaaatatg
    33721 tctgacggta accaaatgca gcaatacatt tgttttagca atacaattgc tgcacactat
    33781 tctaaaagcc gcataatatc ttagcaagct aattataagg agatgaaatt ggaatcgcca
    33841 ctaggttctg atctggcacg gttggtgcgc atatggcgtg ctctgataga ccatcgcctg
    33901 aaaccgctgg agttaacaca aacccattgg gttacgttac acaatatcca tcagttacct
    33961 ccagaccagt cgcaaattca actggcaaaa gcgattggca tcgagcagcc atcactggtc
    34021 cgtactctgg accaactgga agaaaaaggg ttaatttcgc gtcaaacttg tgccagcgat
    34081 cgtcgggcta aacgtattaa actgacggaa aaggcagagc cactgatcag cgaaatggaa
    34141 gctgttatta acaaaacccg cgcggaaata ttacatggca tctccgcaga ggaactggag
    34201 caactgatta cgctcatcgc aaaacttgag cataatatca ttgagttaca ggccaaaggg
    34261 tgaaatgaag ggggcgtgtg gtcagtaacc tgaccacacg caaacttatt tagcgcggag
    34321 aaacggtcac ctgactgcca ttgctggcca gtacgacacg ttggcccgga gagaaacgag
    34381 tgttgccttg tttctgtacc accatgatgg tattaccatc gtctttacga atttccagct
    34441 cgacaccctg cgttttgttc attgcactct gtacgccctg accagctacg ccacctgcaa
    34501 cagcgcctgc tgcagtagcc agagaacgcc cggttccgcc accaacagta ttccccagga
    34561 aaccaccaag aacagcaccg ccaattgcac cgataacgtt ggaatcatca ccgccctgaa
    34621 tctgtaccgg acgtacgtta acgatggtgc catagctgac attctgtact tgtttcgctt
    34681 cagaagcggt ataaacatcc cctgacaggg tgtcgttatt aacacaaccg acaagagaca
    34741 gacctaccat tgaaacaacc aatacgcgtt taatcattga aaaatctcct gttcaccatg
    34801 aaacgccacg caagcatccc tcatggtcaa agtatatggc atatgtgaat ccatggttac
    34861 atgttctacc aaatatgagc aaatcataat gaaagtagga tcaaccaatt ataaacaaaa
    34921 cattcatgat gattattgat ttgaagataa tgttttacta ccaacggtaa aaatcggtca
    34981 ttccgcatgg tattcacgaa aagtttatgt ttgagtggtg cgcaaacgtc agattgaaaa
    35041 aggatgaact atgaaatcgg gccgctttat tggcgttatg tcaggcacca gccttgatgg
    35101 tgttgatgtt gtgttggcga caatagatga acaccgggtc gcacagctgg caagcttgag
    35161 ctggccgatc ccggtgtctc tgaaacaggc tgtactggat atttgccagg gccagcagct
    35221 tacactttcg cagtttggac agcttgatac tcaactcggg cgactttttg ctgatgcggt
    35281 caatgccttg cttaaggaac aaaacctgca ggcgagagat atagttgcga tcggctgtca
    35341 cggtcaaacc gtctggcatg aaccgacggg cgtggcacca cacactttac agattggcga
    35401 taacaatcaa attgtggcac gcaccggaat tacggttgtc ggtgattttc gccgtcgcga
    35461 tattgccttg ggaggacaag gcgcaccgct ggtacctgcg ttccatcatg ccttgctggc
    35521 tcactcaacc gagcgacgaa tggtgctcaa tattggcggc atcgccaatc tgtcactgct
    35581 cattcctggg cagccggttg gcggctacga taccggtcct ggtaacatgc tgatggatgc
    35641 ctggatctgg cgtcaggccg gtaaacctta cgataaagat gccgagtggg cacgggcggg
    35701 taaagttatt ctcccactgc tgcaaaatat gctcagcgac ccatatttct cgcaacctgc
    35761 accgaaaagc accggacgcg aatactttaa ctacggctgg ctggagcgcc atttacgcca
    35821 ttttccgggt gttgatcccc gtgatgttca ggccacactg gcagaactca ccgccgtgac
    35881 catttctgaa caagttttgt tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg
    35941 tagtcgtaat ccgctactca tggcgcgtct ggcggcatta ctgccaggca cagaagtcac
    36001 caccaccgat gccgttggca ttagtggcga tgacatggaa gcattggctt tcgcctggct
    36061 tgcctggcgg acgctggcgg gattaccagg aaatctgcct tccgtcactg gcgcaagcca
    36121 ggagacggta ctgggggcta ttttccctgc taacccgtga tatcagagtt aactgaattt
    36181 ttcttaccgc gtctgccgtt acacttggaa cattagggag ggcgtattgc cctccagacc
    36241 aggaaagtct tcgggaaatg tctatgaaaa aactgttact gatctgtttg ccggtgcttc
    36301 tcaccggctg tagcgcgttc aatcaactgg ttgagcacat gcaaaccgct acgctggaat
    36361 accagtgtga tgaaaaaccg ttgacggtca aactgaataa tccgcgccag gaagtcagtt
    36421 ttgtttacga taatcaacta ctgcatctca aacagggcat ttcagcgtct ggtgcacgtt
    36481 acactgacgg aatctatgtt ttctggtcga aaggcgatga agcgactgtc tataaacgcg
    36541 accgcatcgt attgaataac tgtcagttac aaaatccaca gcgttgagat ttttccaggg
    36601 gcggcgcaca atagcgtcac ccactgacaa tccgtaaaga aaaccatgtc tgataacgac
    36661 gaattgcagc aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt acgccgtcgc
    36721 gatcttcccg ccgatccatt aacccttttt gaacgttggc tctctcaggc gtgtgaagcc
    36781 aaactggcag accctaccgc gatggtggtc gctaccgtgg atgaacatgc tcagccttat
    36841 caacgcatcg ttttactcaa acattacgac gaaaaaggca tggtgtttta caccaacctc
    36901 ggtagccgta aagcacatca aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg
    36961 catacccttg aacgccaggt gatggtgatc ggtaaagcag aacgactttc gactctcgaa
    37021 gtgatgaaat attttcatag ccgcccgcgt gatagccaga ttggtgcatg ggtttcgaag
    37081 cagtccagtc gcatttctgc ccgcggtatc cttgaaagta aattcctgga gctgaagcag
    37141 aagtttcaac agggcgaagt gccattgccg agcttttggg gcggttttcg cgtcagcctt
    37201 gaacagattg agttctggca gggcggtgag catcgcctgc atgaccgctt tttgtaccag
    37261 cgagaaaatg atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg
    37321 ctttaatcgc tggtactcct gattctggca ctttattcta tgtctctttc gcatctggcg
    37381 aaaagtcgtg taccggcaaa ggtgcagtcg ttatatacat ggagattttg atggcaagca
    37441 gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag
    37501 cgttagcaga gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg
    37561 ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg
    37621 cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc gacccgagct
    37681 tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa
    37741 tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg
    37801 cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc gatattggca
    37861 aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg
    37921 aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg
    37981 cctgtctgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac cagtggggta
    38041 acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg tttggcctga
    38101 ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa ggcggcgcag
    38161 tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg atcaacactg
    38221 cggatgccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt gaagagatca
    38281 acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg
    38341 cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca aaacgtatta
    38401 ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc gaacagctgg
    38461 cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg caggcgctgg
    38521 tcgattctga actgcaacct tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca
    38581 tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc
    38641 tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg atttgctgga
    38701 aataatgcat taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg
    38761 aacatgatga aaaatattct cgctatccag tctcacgttg tttatggtca tgcgggtaac
    38821 agtgcggcag agtttccgat gcgccgtctg ggcgcgaacg tctggccact gaacaccgtt
    38881 caattttcta atcacaccca atacggcaaa tggactggct gcgtgatgcc gcccagccat
    38941 ttaaccgaaa ttgtgcaagg cattgccgcc attgataaat tacacacctg tgatgccgtg
    39001 ttaagtggct atctgggatc ggcggagcag ggtgaacata tcctcggtat cgtccgtcag
    39061 gtgaaagccg cgaatccgca ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa
    39121 aaaggctgta tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc
    39181 agcgatatca ttgcgccgaa tctggttgag ctggaaatac tctgtgagca tgcggtaaat
    39241 aacgtcgaag aagcggttct ggcagcgcgc gaactcattg cgcaagggcc acaaattgtg
    39301 ttggttaaac acctggcgcg agctggctac agccgtgacc gttttgaaat gctgctggtc
    39361 accgccgatg aagcctggca tatcagccgt ccgctggtgg attttggtat gcgccagccg
    39421 gtaggtgttg gtgatgtgac gagcggttta ctgctggtga aactgcttca gggggcaacg
    39481 ctgcaggagg cgctggaaca tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa
    39541 gcaatgcagg aatatgagct gcaagtggtg gctgctcagg atcgtattgc caaaccagaa
    39601 cattacttca gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gacctgtggg
    39661 ccgatggata ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag
    39721 ccatacgttg cataaatgct gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt
    39781 atgcccagcg cagaaccgta aacagatagg catcagcaat tgtaaatctt tgcccgcaga
    39841 tccagtgctc atccttcagt gcctcgttca catattgcag cttcttctcc agctgcgcgc
    39901 gaactgtcgg tttgtactct tccggtgtat cagggcgaaa cagaggtgtg aaacctttat
    39961 gcagctcggt ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta
    40021 ccggtgccag caactggcgg tcggggacgc tgtcggcaag atactgcata atcgctacgc
    40081 cttccgtcag caaagtaccg tcatccagca gcaatgcagg cacctgcccc ttagggttaa
    40141 cggcaaagta atcgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgaggg
    40201 taaaatcctt tccgctctca cgcagggtga tatgggaagc gagagagcag gcacccggtt
    40261 tgtagaacaa tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg
    40321 ctgaacaaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
    40381 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
    40441 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
    40501 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
    40561 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
    40621 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
    40681 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
    40741 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
    40801 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
    40861 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
    40921 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
    40981 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
    41041 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
    41101 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
    41161 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
    41221 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
    41281 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
    41341 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
    41401 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
    41461 catagcatgt agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa
    41521 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
    41581 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
    41641 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
    41701 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
    41761 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
    41821 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
    41881 acacttttac ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct
    41941 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
    42001 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
    42061 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt
    42121 tcactgcact gagagggggg tgatggctta acggcgatat gttctgctat ttagcgtttt
    42181 tactagtgag atagtccagt ttctgaaaaa tagccagtgt aatgttttgt aggtcaatat
    42241 ttgttgcgtg tgtaaattta aatgatttta acctgattct tgcaatgttt ttacattgcg
    42301 acagcatcgt gatcttgatc acgaatgaat agaaaatttc gctattaaaa aaacaattaa
    42361 cctgcatttt tggttatccg caaacggatt acctgatgat aacgctaagc aatggcatca
    42421 ttgacgttaa aaagatgttt taaaagagga taaagaaagg ttatcaatgg ggtaatcggt
    42481 gttacccctt ttcttcagat gtcaactttc tctttgtatt cacaaagatc ttcaataata
    42541 caagagccac agcggggctt gcgggcaatg caggtataac gcccgtgcag gatcaaccaa
    42601 tggtggcagt cgactttaaa ctctgccgga accactttca gtagcttttc ttctacctgt
    42661 tcgacgtttt tccccggcgc aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg
    42721 acagcaatag tcggccagcc gaatgcagtg tttaatacga cgttggctgt tttacgacct
    42781 acgccgggca gggcttcaag cgcagcacga tcttccggaa cctcgccatt atgctgctcc
    42841 agtaagatac ggcaggtttt gatgatattt tctgctttgc tgttataaag cccaatcgtt
    42901 ttgatatagg ttttcacccc ttcaacgccc agttcaagca tcgctgcagg cgtattcgcc
    42961 accgggtaga gtttcgccgt cgccttatta acactgacat cggtcgcctg agcggaaagc
    43021 agtacggcaa tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga
    43081 ttgttctcac gcaggcgagt gaggatctcc aggcgttttg ctttgttcat cagacattcc
    43141 ctgtttcacc gtttggcaat gcacgttctg cagctgcttc agcacggcgc tttttcattc
    43201 tttcatcaat caggtatttt cctgccagca tcagtcccag gccaataaat gcacctggag
    43261 gcagcatcgc cagcaggaaa ggggagtcgg tgtggaaaat ttccacgcgt aatacttttg
    43321 cccagctacc taacagcgca tctgcaccgt caaacaatgt gccattgccg ataatttcgc
    43381 gtagtgaacc cagcacgaac atggcgcagg ttgcgcccat accaattgaa aagccgtcca
    43441 gtgccgaaag cgccggacct tttttggcgg cgaaggcttc agcgcggccc acaacgatac
    43501 agttagtgac aatcagcgga ataaaaatcc ctaatgattg atacaggcca aaggcgtagg
    43561 cgttgatcag catctgtaca gcgctgacca ccgaggcgat gatcatcacg taaatgggaa
    43621 tgcggatctc ggctggcgtc cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca
    43681 gtaccagcgt agtcgcaagt cctaaaccca gagcgttagt ggcagtggac gtgaccgcca
    43741 acagaggaca aaggccgagc aactggacca gcgcagagtt gtttttccac aacccctgaa
    43801 caataacgtc tttaatttcg ctcacgcttt attctccaca ggcaggaagt tgagaaagtt
    43861 gtgccggtaa cgtctgagcg tacaatccgg cgcgttttac cgcattaacc accgcgcggg
    43921 gagtaatcgt cgcgccggtg aactggtcga aatcaccacc atctttcttc accgcccagt
    43981 gcgcatcatc tgcaccactg atttttttac ccgcaaaatg ggtgatccag tcagaaaggc
    44041 gcagttcgat tttatcgcca agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa
    44101 gtaccgtgcc gttaaaatcg gctcccacca gcagctgaat cgcaccggaa tagccatctg
    44161 gcgcggttgc ttccagaacg gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc
    44221 gatgctcacc tttacctaac tctggcgcag ttaccagata gcaactctgt gccagcgcat
    44281 tgttatagcg ttcggctggc agcacctgat caaataatgc cttttgttgc agactggcct
    44341 gttcagcaat cgtcgttttg gtcatctggt tgatggccgc agttaaccct gttgaacccg
    44401 ctgcaaatag cgctaacgta atgccgtgtt ttcggatagt tttcagcatg gtttaccctt
    44461 tgcgatggcc gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg
    44521 ccagcaggac ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg atcaaccaga
    44581 ctaataagcc cgcaagcgcg ccgaaaataa gacgaccacg attggtcgta gaagcggtaa
    44641 ccgggtcagt caaaataaag aatgcgccga gcatggtcgc accagacagc agatgaattt
    44701 gcggtgctgc cagtgtttct ggtgagaaca accagcccaa cgttgcgcat aacgccagcg
    44761 ttactaagaa gctgagggga atatgccagc gaatcgcttt ctgccatagc aaccacaggc
    44821 cgccagccag ccaggcgaga tttacccatt gccaaccagc gcccgccaga ataccgctgt
    44881 agatcggata ttgcataatc tgttcaaccg aatgaccggc acggacagag gttttaaatg
    44941 tatccagcgg tgtcgcctga ctaatgccat caatacctaa gcgtagtgtg ttcatatcac
    45001 caccactggc ggtatgcccg ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt
    45061 tgaccgcaat ttcatgtggc ggtaaccagc tggtcatctg caccgggaag gagatcagta
    45121 agaccacata accaatcatt gctggattaa acgggttttg ccccagaccg ccatacaact
    45181 gtttagcgat gatcaccgca aacaccgtac ccagcacgac catccaccat ggcgcgaggg
    45241 ggggaatact taccgccagc aataagcctg tcagcaatgc tgagttatct ttcaacgttg
    45301 cggctaccga ctgcttgcgt agtttgagta cgagagcttc ggctaacaga gcactaaccg
    45361 atgccaacag gatctgaacg agagtacccc aaccaaaaaa ccacagttgc gctgcgattc
    45421 ctggcacggc tgcgagcaac accaacagca taatgcgcga tgtctggcgc tggttatgga
    45481 tataagggga gctagctatt ctgaatacca tttagtcctc gtttacaacc ttctgctggg
    45541 cggctttttt ggcctgagcg cgggcaatag ccgcggcaac tgccgctttg cgcggatcaa
    45601 cctgttcttc tggttctgcg ttagtctgtt gctgttccag cttgcgcgct ttggcacggg
    45661 caatagcggc ttcgacggcg gctttgcgcg gatcaacctg ttcttctggt tccgcattag
    45721 cctgttgctg ttccagtttg cgcgctttgg ctcgggcaat agcggcttcg acggcggctt
    45781 tgcgcggatc aacctgttct tctggttccg cattag
//
LOCUS       DAPHDX010000019        45547 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPHDX010000019 DAPHDX010000000
VERSION     DAPHDX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.019"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            119..745
                     /gene="upp"
                     /locus_tag="RH058_001034"
     CDS             119..745
                     /gene="upp"
                     /locus_tag="RH058_001034"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV3136657.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            831..2120
                     /gene="uraA"
                     /locus_tag="RH058_001035"
     CDS             831..2120
                     /gene="uraA"
                     /locus_tag="RH058_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV3136658.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            2170..2916
                     /gene="hda"
                     /locus_tag="RH058_001036"
     CDS             2170..2916
                     /gene="hda"
                     /locus_tag="RH058_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV3136659.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3054..3413)
                     /gene="arsC"
                     /locus_tag="RH058_001037"
     CDS             complement(3054..3413)
                     /gene="arsC"
                     /locus_tag="RH058_001037"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV3136660.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3434..4897)
                     /gene="bepA"
                     /locus_tag="RH058_001038"
     CDS             complement(3434..4897)
                     /gene="bepA"
                     /locus_tag="RH058_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV3136661.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            5110..6171
                     /locus_tag="RH058_001039"
     CDS             5110..6171
                     /locus_tag="RH058_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV3136662.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(6209..6433)
                     /locus_tag="RH058_001040"
     CDS             complement(6209..6433)
                     /locus_tag="RH058_001040"
                     /inference="COORDINATES: protein motif:HMM:NF013397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV3136663.1"
                     /translation="MYRQFVCDSVRHCHSPFCPTPLRQLAHSSADNFPALTVSHFITA
                     NLLPVMLGNIIGGAVLVSICYRAIYLRQES"
     gene            complement(6325..7056)
                     /locus_tag="RH058_001041"
     CDS             complement(6325..7056)
                     /locus_tag="RH058_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDV3136664.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIASHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPRPFGNWRTVAQTIFRH"
     gene            complement(7078..9090)
                     /gene="hyfR"
                     /locus_tag="RH058_001042"
     CDS             complement(7078..9090)
                     /gene="hyfR"
                     /locus_tag="RH058_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV3136665.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSLVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNNLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(9120..9533)
                     /gene="hyfJ"
                     /locus_tag="RH058_001043"
     CDS             complement(9120..9533)
                     /gene="hyfJ"
                     /locus_tag="RH058_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV3136666.1"
                     /translation="MAEECGEIVIWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(9526..10284)
                     /gene="hyfI"
                     /locus_tag="RH058_001044"
     CDS             complement(9526..10284)
                     /gene="hyfI"
                     /locus_tag="RH058_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDV3136667.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(10281..10826)
                     /gene="hyfH"
                     /locus_tag="RH058_001045"
     CDS             complement(10281..10826)
                     /gene="hyfH"
                     /locus_tag="RH058_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV3136668.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSHTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(10836..12551)
                     /gene="hyfG"
                     /locus_tag="RH058_001046"
     CDS             complement(10836..12551)
                     /gene="hyfG"
                     /locus_tag="RH058_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV3136669.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(12541..14126)
                     /gene="hyfF"
                     /locus_tag="RH058_001047"
                     /pseudo
     CDS             complement(12541..14126)
                     /gene="hyfF"
                     /locus_tag="RH058_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            complement(14131..14781)
                     /gene="hyfE"
                     /locus_tag="RH058_001048"
     CDS             complement(14131..14781)
                     /gene="hyfE"
                     /locus_tag="RH058_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV3136670.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(14793..15900)
                     /locus_tag="RH058_001049"
                     /pseudo
     CDS             complement(14793..15900)
                     /locus_tag="RH058_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(15912..16022)
                     /locus_tag="RH058_001050"
     CDS             complement(15912..16022)
                     /locus_tag="RH058_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HDV3136671.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(16039..16986)
                     /gene="hyfC"
                     /locus_tag="RH058_001051"
     CDS             complement(16039..16986)
                     /gene="hyfC"
                     /locus_tag="RH058_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV3136672.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16997..19014)
                     /gene="hyfB"
                     /locus_tag="RH058_001052"
                     /pseudo
     CDS             complement(16997..19014)
                     /gene="hyfB"
                     /locus_tag="RH058_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(19014..19631)
                     /gene="hyfA"
                     /locus_tag="RH058_001053"
     CDS             complement(19014..19631)
                     /gene="hyfA"
                     /locus_tag="RH058_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV3136673.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(19884..20354)
                     /gene="bcp"
                     /locus_tag="RH058_001054"
     CDS             complement(19884..20354)
                     /gene="bcp"
                     /locus_tag="RH058_001054"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV3136674.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(20354..20926)
                     /locus_tag="RH058_001055"
     CDS             complement(20354..20926)
                     /locus_tag="RH058_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV3136675.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            21072..21950
                     /gene="dapA"
                     /locus_tag="RH058_001056"
     CDS             21072..21950
                     /gene="dapA"
                     /locus_tag="RH058_001056"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV3136676.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21967..23001
                     /gene="bamC"
                     /locus_tag="RH058_001057"
     CDS             21967..23001
                     /gene="bamC"
                     /locus_tag="RH058_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV3136677.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            23214..23927
                     /gene="purC"
                     /locus_tag="RH058_001058"
     CDS             23214..23927
                     /gene="purC"
                     /locus_tag="RH058_001058"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV3136678.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            24081..24308
                     /gene="vapB"
                     /locus_tag="RH058_001059"
     CDS             24081..24308
                     /gene="vapB"
                     /locus_tag="RH058_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV3136679.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            24308..24706
                     /gene="vapC"
                     /locus_tag="RH058_001060"
     CDS             24308..24706
                     /gene="vapC"
                     /locus_tag="RH058_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV3136680.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24853..25716
                     /locus_tag="RH058_001061"
     CDS             24853..25716
                     /locus_tag="RH058_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV3136681.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25731..27746
                     /gene="tmcA"
                     /locus_tag="RH058_001062"
     CDS             25731..27746
                     /gene="tmcA"
                     /locus_tag="RH058_001062"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV3136682.1"
                     /translation="MAELTAFHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIAAPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHTATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            27820..28518
                     /gene="ypfH"
                     /locus_tag="RH058_001063"
     CDS             27820..28518
                     /gene="ypfH"
                     /locus_tag="RH058_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3136683.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(28599..28799)
                     /locus_tag="RH058_001064"
     CDS             complement(28599..28799)
                     /locus_tag="RH058_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV3136684.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28827..29954)
                     /gene="dapE"
                     /locus_tag="RH058_001065"
     CDS             complement(28827..29954)
                     /gene="dapE"
                     /locus_tag="RH058_001065"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV3136685.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMSRNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29958..30314)
                     /locus_tag="RH058_001066"
     CDS             complement(29958..30314)
                     /locus_tag="RH058_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV3136686.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLKG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            30695..30754
                     /gene="ypfM"
                     /locus_tag="RH058_001067"
     CDS             30695..30754
                     /gene="ypfM"
                     /locus_tag="RH058_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV3136687.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(30853..33966)
                     /gene="acrD"
                     /locus_tag="RH058_001068"
     CDS             complement(30853..33966)
                     /gene="acrD"
                     /locus_tag="RH058_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV3136688.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(34130..35830)
                     /gene="narQ"
                     /locus_tag="RH058_001069"
     CDS             complement(34130..35830)
                     /gene="narQ"
                     /locus_tag="RH058_001069"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV3136689.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            36036..38015
                     /gene="aegA"
                     /locus_tag="RH058_001070"
     CDS             36036..38015
                     /gene="aegA"
                     /locus_tag="RH058_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV3136690.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            38083..38658
                     /gene="nudK"
                     /locus_tag="RH058_001071"
     CDS             38083..38658
                     /gene="nudK"
                     /locus_tag="RH058_001071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV3136691.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            38784..39827
                     /locus_tag="RH058_001072"
     CDS             38784..39827
                     /locus_tag="RH058_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV3136692.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(39923..41926)
                     /gene="tkt"
                     /locus_tag="RH058_001073"
     CDS             complement(39923..41926)
                     /gene="tkt"
                     /locus_tag="RH058_001073"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV3136693.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(41946..42896)
                     /gene="tal"
                     /locus_tag="RH058_001074"
     CDS             complement(41946..42896)
                     /gene="tal"
                     /locus_tag="RH058_001074"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV3136694.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            43185..45464
                     /gene="maeB"
                     /locus_tag="RH058_001075"
     CDS             43185..45464
                     /gene="maeB"
                     /locus_tag="RH058_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV3136695.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLLSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT        11029 a        11834 c        12149 g        10535 t
ORIGIN      
        1 ctttatctca aaccgttatc attttgacta aagtcaacga aaagaatatt gccgccttga
       61 agaaaggagg tataatccgt cgattttttt tgtggctgcc cctcaaagga gaaagagtat
      121 gaagatcgtg gaagtcaaac acccactcgt caaacacaag ctgggactga tgcgtgagca
      181 agatatcagc accaagcgct ttcgcgaact cgcttccgaa gtgggtagcc tgctgactta
      241 cgaagcgacc gccgacctcg aaacggaaaa agtaactatc gaaggctgga acggcccggt
      301 agaaatcgac cagatcaaag gtaagaaaat tactgttgtg ccaattctgc gtgcgggtct
      361 tggtatgatg gacggtgtgc tggaaaacgt tccgagcgcg cgcatcagcg ttgtcggtat
      421 gtaccgtaat gaagaaacgc tggagccggt accgtacttc cagaaactgg tttctaacat
      481 cgatgagcgt atggcgctga tcgttgaccc aatgctggca accggtggtt ccgttatcgc
      541 gaccatcgac ctgctgaaaa aagcgggctg cagcagcatc aaagttctgg tgctggtcgc
      601 tgcgccagaa ggtatcgctg cgctggaaaa agcgcacccg gacgtcgaac tgtataccgc
      661 atcgattgat cagggactga acgagcacgg atacattatt ccgggcctcg gcgatgccgg
      721 tgacaaaatc tttggtacga aataaagaat aaaaataatt aaagccgact ttaagagtcg
      781 gctttttttt gagtaaagcg cctataacac ataatacaga ggataatact atgacgcgcc
      841 gtgctatcgg ggtgagtgaa agaccgccac ttttacagac aatcccgctt agtttgcaac
      901 atttgttcgc catgtttggt gcaaccgtcc tggtgcccgt cttatttcat attaacccgg
      961 cgactgtact gttatttaac ggtattggaa cgctgctgta tctcttcatc tgtaaaggga
     1021 aaattccggc ttatcttggt tccagctttg cctttatttc accggtattg ttactgttgc
     1081 cgttagggta tgaagtcgcg ctgggcggct ttattatgtg cggcgtgctg ttctgcctgg
     1141 tttcttttat cgtgaagaaa gcggggaccg gctggctgga cgtgctgttt ccaccagcgg
     1201 caatgggcgc aatcgttgcc gtcatcggtc tggagctggc gggcgtagct gccggtatgg
     1261 cgggtttact cccggctgaa gggcaaacgc cagactccaa aaccatcatc atctctatta
     1321 ccaccctggc ggtcacggtt ttaggttccg tgctgtttcg tggtttcctg gcaattatcc
     1381 cgattttaat tggcgtgctg gtgggctacg cgctctcttt cgcaatggga attgtcgata
     1441 ccacgccgat tattaatgct cactggtttg cgctgccaac cctctatacg ccgcgcttcg
     1501 agtggtttgc cattctgact attctgccag cggcgttagt ggttattgcc gaacacgtag
     1561 ggcacctggt agtaacggct aatatcgtca aaaaagatct gctgcgcgat ccaggtctgc
     1621 accgttcgat gtttgctaac ggcctgtcga ccgtgatctc cggcttcttt ggctctacgc
     1681 caaataccac ttacggagaa aacattggcg tgatggcgat cacccgtgtt tacagtacct
     1741 gggttatcgg tggggcggcg attttcgcta tcctgctttc ctgtgtcggt aaactggctg
     1801 ccgctatcca gatgatccca ttgccggtga tgggcggcgt ttcgctgctg ctttatggtg
     1861 tcatcggtgc ttccggtatt cgtgttttga tcgaatcgaa agtggattac aacaaagcac
     1921 agaacctgat cctgacttcc gtgattttga tcatcggcgt cagtggcgcg aaggtaaaca
     1981 tcggcgcggc ggagttaaaa ggtatggcgc tggcgaccat cgtcggtatc ggcttaagtt
     2041 tgatcttcaa actgattagt gtgctgcgcc ctgaagaagt ggtgctggac gcagaagacg
     2101 cggatataac agacaaataa tgtaccgacc gggcagtgtt gctgcccggt tcaaacatca
     2161 tgggattctg tggtaaactt ctcgcgattt tgtgaaatcc tggttgaggt atctctgaac
     2221 acaccggcac agctctcttt gccactttat cttcctgacg acgaaacctt tgcaagtttc
     2281 tggccggggg ataactcctc tttactggct gcgctgcaaa acgtgctgcg tcaggaacat
     2341 agcggttaca tctatctctg ggcacgcgaa ggcgcggggc gcagccatct gctgcacgcg
     2401 gcttgcgcgg aattgtcgca gcgtggcgat gcggtgggct atgtcccgct ggataaacgc
     2461 acctggtttg ttccggaagt gctcgacggt atggagcatt tgtcgctggt ctgtatcgac
     2521 aacattgagt gtattgcagg cgatgagttg tgggagatgg cgattttcga tctctacaat
     2581 cgaattctgg aatcgggcaa aacacgattg ttgatcaccg gcgatcgtcc accgcggcag
     2641 ttgaatctgg gattaccgga tctcgcgtcg cgactcgact gggggcagat ctacaaattg
     2701 cagccacttt ctgatgaaga taagttgcag gcgctacagt tacgcgcgcg tttgcgtggt
     2761 tttgaactgc cggaagatgt ggggcgtttc ttgctgaagc ggctcgacag agaaatgcgc
     2821 acgctattta tgacgttgga tcagttggat cgtgcgtcga ttaccgcgca acgtaagctg
     2881 accattccgt ttgtgaaaga aattctgaag ttgtagataa ggtgtttatt gtcggatgcg
     2941 gcgcgaacgc cttatccgaa ctacgcgaag ttaatccata gtattcgtag gccggataag
     3001 acgtggcaag cgtcgcatcc ggcaatgtgc ttcaactagc gatggagcac atcttaaccc
     3061 acgatttcca gtacctgctc cggtggacga ccaatccgcg ctttgccatt cgcgaccaca
     3121 atcgggcgct ccattagctt cggattatcc accattgcct gaatcagcgc ctcttcgcta
     3181 agcgaactgt cagccaggtt cagttcttta taaagatcct ctttctggcg catcaattca
     3241 cgggcgctat tcatccccag cattttcagc aaatcgcgca gcgttgccgc atcggcgggt
     3301 gtctcaaggt agagcaccac ttctggctcc acgccgtttt ctttcagcag gttcagcgtt
     3361 tcccggctct ttgaacagcg tgggttatgg tagattttta cctgtttggt cattgttctt
     3421 cctttaatgc gaattacatc ttggtataag gcttaaagcg ttcctgcagc tggcgcaact
     3481 ggtcgatgcg cgcatcgtaa cgcgcttgtt gcaggctgcc taatttcacc tgcgaactgg
     3541 cgctactcaa cagcgaaatg gcctgatcga gtcgtccggc gagcgcataa ccttctgctc
     3601 gcgcagccag ctcctgatca cggttattta gcgcggcttc cgcctgtgcc agcaaatccc
     3661 agccgttgct gtcatcttta ttattaaagg tgtagcgatt cagaatattg gccgcttctt
     3721 gtggttgacc gccttgcaga taagcgttcg ccaggttgag ctgcaacacc ggattggtgc
     3781 gtaaatcgcg ggcatttttc aggcgattga tcgcttcatt ggctttgttt tgcccaagat
     3841 cgatatcagt agccagatcg agataccatg cgttgccagg ttctgccgcc agtaacggtt
     3901 gcagcgtttt acgcgcctcg tcgtatttat tggcttccat cgcctgtaaa gcacgaccat
     3961 attgcgctgc tcgttgctga cgaacgtttc ctttcgccca ttcatccagc aaatcactgg
     4021 tgagctggtt acgtccggaa ttatacatcc ccagtgtgcg cgctttcgcc agatagaaat
     4081 cttccgacga ctgcaccacc atcgggcgca tctgattagc acggttgcgg gcatctgcca
     4141 gacgactttc cggcaacggg tgagtcaata aaatttccgg cgggcgcgag gagtaacgcg
     4201 cctgatcgag taatttttcg aggaaggttg gcatcgcctg cggatcgaat cccgagcgtt
     4261 gcagcacctg aataccaatg cggtccgctt cctgttcatt ttgctgggtg aaactgatca
     4321 tcccctgacg cgttcccgcc agtgtaccgg tcagcgccgc catccccgcc tgcggactgg
     4381 ccatcgccag taaaatagaa cctaacgcgc cgacccaggt cagcggcgcg ctgcgctgct
     4441 gatcttccat cgctcgcgcc aggtgacgtt gggtgacgtg ggagatttcg tgcgccataa
     4501 ctgaagccag ttgactttcg ttatcggaat aacggaacag ggcagagtgc agcaccacgt
     4561 tgccgccaaa gaaagcaaag gcgttaattt cgtcgttgtt gatcagaaaa aaatgaaacg
     4621 gtgtcttaac cgaattggca tgcgaaacca gacgcatccc cagcgaatta atatattgcg
     4681 ttaacagcgg gtcattaatt aacggcgcgc tgccgcgtag ctggcggaca taatagtcgc
     4741 ccatctgcat ttcctgacca atggaaagcg tgcttcctgc ggaggttccc atatccggca
     4801 aggtgtctgc gctgtcggca aacgccgggg ctacctgacc aatggtcatt gcagcaatga
     4861 gggttgcaac caggtttttt ttcaactgcc tgaacataac ctctatcctg tatttctgaa
     4921 gatagtcatt tgaccgatac tgtgaaatat cgttcccacc gcgccgcctt tgagtgtaac
     4981 ggcgaagatt agcccactca aatgactttt cagtttcctg aatccgtcat aaaaagcgaa
     5041 gtcttttttg tgacatttcg atacaattgc ggatcgcaat cccgctcttg aggttcaggg
     5101 aaggtcttta tgctcgaaat gttgatgcaa tggtatcgcc gccgttttag cgacccggaa
     5161 gcgattgcct tgctggttat tttagttgcc ggatttggca ttatcttttt ctttagtggc
     5221 ctgcttgctc cgttgctggt ggctattgtg ctggcctatt tgctggaatg gccaaccgtg
     5281 cgcctgcaat ctattggctg ctcccgccgc tgggcgacgt cgattgtatt ggtggttttc
     5341 gtcggtatat tgctactgat ggcgttcgtg gtactgccta tcgcctggca acagggcatc
     5401 tacttaatcc gcgatatgcc ggggatgctc aataagcttt ctgactttgc cgccacgttg
     5461 ccgcgccgct atccggcgtt aatggatgcg ggcattattg atgcaatggc cgaaaatatg
     5521 cgcagtcgga tgctgactat gggcgattcg gtggtgaaaa tttccctcgc ctcgctggtc
     5581 ggtttgctga ccatagccgt ctatctggtg ctggtgccat tgatggtctt cttcctgctg
     5641 aaagacaaag agcagatgct gaacgccgtt cgccgggtgc tgccgcgtaa ccgtggactg
     5701 gcaggacagg tgtggaagga gatgaatcaa caaatcacca actatatccg cggcaaagtg
     5761 ctggagatga tcgtagtagg gatcgccacc tggctggggt tcttgctctt cgggctgaac
     5821 tattcgctgc tgctggcggt gctggtcggc ttctcggttc ttattccgta cattggcgca
     5881 tttgtggtga ccattccggt ggttggcgtg gcgctattcc agtttggcgc aggtactgaa
     5941 ttctggagct gcttcgcggt gtatctgatt attcaggcgc tggacggcaa cctgttagta
     6001 ccggtgttgt tctccgaagc ggttaacctg catccgctgg tgattatttt atcagtagtg
     6061 atcttcggtg gtttgtgggg attctggggc gtattcttcg ccattccatt ggcgacgctg
     6121 atcaaagccg tgatccacgc ctggcccgat gggcaaatcg cgcaagaata atcttcagcc
     6181 cgacgaactg tttcgccggg ctgatttttc aggattcctg acgtaaataa atagcccgat
     6241 aacaaatact caccagcact gcaccgccga taatattacc cagcatcacc gggagcagat
     6301 tggcggtaat aaaatggctg accgtcagtg ccggaaaatt gtctgcgcta ctgtgcgcca
     6361 gttgccgaag gggcgtgggg caaaatggcg aatggcaatg gcgaacggaa tcacaaacaa
     6421 attggcgata cagtgctcaa agccactggc gacaaacagg gtgatgggca aaatcatagc
     6481 gacgattttg tcgcataatg aacgcccgca ataactcatc cacagcgcca ggcaaaccat
     6541 cagattgcac ataatgccga ggctgacaga ttcagtaaat gtatgatgca ttttgccctc
     6601 ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca ctcatcacca gcccggaaaa
     6661 ccagattaac aaactgaaac aggcaatacc tgccagatta ccgcaggcca ccagaagtgc
     6721 gttaatcagc caagttcgcc aactaataac gccccgactt tttgccatca ccgtcattac
     6781 cgacgaggtg aacaggctgg tgccacaaac cgccagcaaa ataaagccga gtgtaaagca
     6841 aaggccgccc accagatggg ttaatgcctg tgaagacggg gcatcggcga taacggaaag
     6901 gtaaaaagta aatccgatcg ccataaacac gccagcggac atcgccatta aaaagaccga
     6961 cactttactg cgggcaattt tatgggaggc aatccgttca gcgattgccg cttttctggc
     7021 gctcaactgc aagtcgaaag agagtttgtt tctcatgcat actcctgcaa aagcagatta
     7081 caacacctcg cgaaccgaga tccccagacg ctgcattcgt gacagcagcg tggtgcgctt
     7141 catccctaat cgtgtcgccg cgccacgggg cccggcaaca atgccattgg tttctcgcaa
     7201 tacctgaaca atgcgctgac gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa
     7261 ctgcgccatt gaactgcgaa gcgctgaatc ttcatttagc gtcggcagta aacggctttg
     7321 tcggacattt agatgtaaat tcagattgtt accacgagtc aacagtaccg cccgctcaat
     7381 cacgttttcc agctcgcgca cgttgcccgg ccaatcccac gacatcaact ggcgtagcgc
     7441 ctcggtcggg attgcgtcaa ttgcgcgatt catatggcgc gccatttttt gcgtgaaatg
     7501 ctttgctaaa agagggatat cttccggacg atcgcgtagc ggcggcaatt ccagtgggaa
     7561 gacattcagg cgataaaaga gatcgctgcg aaactggcga tcttcaacca tttgccacaa
     7621 atcacggttg gtggcggcaa tgactctgac attcaccggg atcgttctac tcccgccgag
     7681 acgctcaatc tcccgctcct gcaatacgcg cagcaattta ggctgaagtt ctaacggcag
     7741 atcgccaatt tcatcgagaa acaacgtacc gccatcggca atttcaaaac gaccacgatg
     7801 ggtattaatc gcaccagtaa acgccccttt gtcatgaccg aataactcac tttccagaag
     7861 actggcgggg atggcagcgc agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag
     7921 ttgatggatc gctctggcga tcacctcttt gccagttccg gtttcaccgc aaatcaacac
     7981 cgtactgtcg ctcttcgcca caatatctac ctgctggagc aggtcttcca ttgcctggct
     8041 ttgatagata atgtcaccga cgcccagatt cgaaaggagc tgctcgctaa gctggtggtt
     8101 ttcttgctgc aaactttcct gcaaatcggt catgctacgc caggcatcgg cattgccaac
     8161 ggcaatagcg atgcgatcgg ctatgtgttg tagtagctgg cagttttcct cactaaagag
     8221 agtggaagag gtatgcgcca gtaacaatgc acccggtgta tggttgccaa aggtaagcgg
     8281 tatcaggagc gcagattcgc agccgttaga tgcaagtaag agtaataacg ggtcgcgttg
     8341 ccagagaaac agatcgtctg cacggtgcgt caaggtcggt tgtcgggttt gtagcgtttg
     8401 tgtcagcaat acactgtcgc caggcatatt gcgtggcaga cacgcacaat gtgaggcaga
     8461 aagatcgctg caccacaggc tgaacttctc gttctttcga tgatcgccca gtaccatact
     8521 gactgaagcc agaccgaaaa aatgatggat ctcacgagcg acgtcagcga tcagatcgtc
     8581 gagatcaaga tgtgacagca ctgcattagt gatatccacc agtacccgat gatgatcgcg
     8641 atctttctcc agcaaacgca gcgccacctg ttgttcttca tcattgagcc atgattgcag
     8701 aactggggtg ataatgcgtg ccagccagtt aaacagtgtg caatcctggg gagtaaaacg
     8761 gctgccgtgg gtacgaataa agcgtagctc cgccattcca ctactgccga gaaggacggc
     8821 gtgacgcgaa taatcgacca tctcgccctg atactctact gttgccgtca catcgctgcc
     8881 atcatcatgg tagcggtaat agaagttgct tttgagaaca tgcagttcaa taagttgtac
     8941 caggctgaac ggcggcggca gcgggatgaa ggcgcgtaat aaagacgcct tgccgtgttt
     9001 ctgtgctaac cgctccgcga gcattccgtt taccgcttca attgttattc cttgtggcgg
     9061 ggcaaacatc gcctcgtctg acatagccat caacagtcct tattaatgag aaatccttct
     9121 cacgcaattt ttctggccat cagatagatg gccggttcct gcacaatttc atccaacaga
     9181 tgaatgaatt gaatgctacg cgcctgccac acagacgtcg cgtcatctgc cagtagcgcg
     9241 aatgcccggg tcaacagggc ggtgtggctg gcgtcaataa caatctcacc aaaggtcatc
     9301 acccccagca tcttacgtcg ggcttgctcc tcttcgacct gtgcaagcca atcttcgtat
     9361 tccgtcagtg ggcagcggaa ggcgacattc agacaatcaa tcacgccaac gtgatgaccg
     9421 atagccagcg agtaatacat tacctgagag ctgtgttccg gcatctcgtc gctactggcg
     9481 acaaactttt ttcgcagcgt ccagataaca atttccccgc actcctcagc catgtaaccc
     9541 ccggagaacg cgaaagagtt gctgaacgat actattgaga cgcggatcgt cggcttcgcg
     9601 caaccaggtg ttaacccgat ttcctgtagg atcgtcgctt aaatggcgca ggagccgatc
     9661 gcaaatttct cgcccctgac gatagcccgc cagccgccgc gcttctcgct caatggcgat
     9721 acgctgtgat ggcgggatct gtggccacaa cggttgcata gtcaccccgg tgggatcgcg
     9781 ataatccaca gcgtgaatct tctgttgcag caaaccgagc gccacggcga aaccgtgaat
     9841 ggtggcggcc ggtgttggcg ggcagccggg gatccaaaca tcaatgggga caatggtgtc
     9901 gctgccgccc cagacgctgt agagatcgtg gaaaataccg ccgccgacac cgcacgcgcc
     9961 gtaggaaaca cagattttat gatcgggggc agactcatac gcccgaagtg caggcatacg
    10021 catcgcccgg gtgactgcgc ctgtaaataa caaaatatcg gcgtgacgcg gtgatgaaat
    10081 aaccttaatg ccaaaacgtt ctgcgtcgaa tactggtgtg atggcggcaa aaatttcgat
    10141 ttcacaggcg ttgcagccgc cgcaatcgac gcgataaacg taagccgagc gacggatatc
    10201 ctgtagcaaa tgctgcttca tcttttgcgt ttgctcgtcc agcgtgatgg gctggctgac
    10261 atgttgtgta agcactggac tcatagctgc tccttagcca ccagcggtac atctgtatcg
    10321 tcgttgagca gcgtcgcgcg ttgtttgcac tccgggcaga cgctcgcttg cgcccataac
    10381 atttcgcggt tttgtggcgc attttgttgc tgtgccaaca attcagcagc cagtgcgacg
    10441 gttttttgcg gggcaaacgg gcgttcgcaa cggctgcaac gttgtagatg gaacgtcgcg
    10501 cgggtataga gatcggcttt attggtgacg gtcagttcaa agttattggt aagctggatg
    10561 gctctggtcg ggcacacttc ttcacaacgt ccgcagtaaa tacaacgcct cagatagagc
    10621 tgccaggtgt gcgaattttg ctggtcgtcg gtctggatag tcagcgcatt tgccggacaa
    10681 gcacaggcgc aggcaccgca ggcaatacat tggctgggca tcaggtccgg tttcccgcga
    10741 aagcctgggc tgacctccag tggcgcgaag ggatatttca ccgtcgcggt tccggcgcgc
    10801 ataatagttt tcagtaactt cagcatcttc tgtccttact tcagcggcga acggttacga
    10861 tcaataccgt agcgttcgat ctctttatac ggcacggttt ttgactggcg cttgcgcaca
    10921 tctaccagcg tcacacggtc ggtacaggag tagcagggat caaggctacc gataatcagc
    10981 ggtgcgtcag aaacggtatt gccgcgcagc atgtaacgca acaccggcca gttggcgtag
    11041 gtggcggcac gacagcgcca gcggaacaat ttttggttat caccgagcat gctccagtgc
    11101 acgtcttcac cgcgtggcgc ttcaacaaag cccagcgcga atgcgtgagg tttatagcta
    11161 aagccttcgg tcagcagcgg ggtatccggc atgttgtcga gggcaaattc cagcattgcc
    11221 agcgaatcaa acgtctcttt gacacggacc atcacgcggg agaaaacatc gccgccggtg
    11281 aaggtaaaca gtgtttttgg aatattgccg tagtcggcgt aggggtgatc aaagcgcaaa
    11341 tcacgggcaa aaccactgcc gcggatcagc ggccctacag ggctataatc acgggcgatt
    11401 tgtcggtcga gaatgccaat gccctgagtg cgttgttcca tattcggcat agcaagcagc
    11461 atctctacca gctccgacac gtcggcgcgc atctcgcgca ccagtttcag cgtttgcaga
    11521 cgttgctctt tgagaatatc gcggcgaaca ccaccaatca gattcagacc gtaggtttta
    11581 cgcgacccgg tcagcaattc cgccatcgtc atcgactttt cccgcacgcg gaaaaattgc
    11641 ataaagccgg tatcaaaacc aacaaaatgg caggagaggc caaggttgag caaatgactg
    11701 tgtagccgtt cgacttccag cagaatcgag cgaatggtat gcgctcgttg cggcacctca
    11761 atccccagtg cattttcaac cgagttggta taggccacac tgtgggcaaa accgcaaatc
    11821 ccacacacgc ggtccgataa gaaggtcact tcgttgtagc ccatccgcgt ttctgccagt
    11881 ttctccatgc cgcgatggac ataaaacagg cggtaatcag catcgacaat ttgctcgcca
    11941 tccacaaaca agcggaagtg acccggttca tcggaggtga tatgcagcgg gccgacaggg
    12001 atcacccgcg caccgctgtt gccctcattg ataaacgcat acgtttcgga atctgtcgtc
    12061 ggttcagggc gcagtcgata atccatcgca tctttgcgca gcggatgcat atcttccggc
    12121 cagtcatcgg gcaacaccag gcgacgctga tccggcaggc caaccggaat cagcccgtac
    12181 atatcgcgaa tttctcgctc gccccagacc gcggcaggga cgcgcggtgt gactgacgga
    12241 aactcccgac tgtcggcatc gaccagtgcc ttcaccacga tccagcattt ttcggcccct
    12301 tccattgaaa gggcataata aaccgcgtaa tgaccgttaa gtgtccgctc gtcgttgcca
    12361 aacaggactg gaagccagcc atcatgttga taataaagat aatgtacaac gtcaggcagc
    12421 agattgattt tcaccgtaat ggtgacctgt tcaggcgttt gtcgctcttc atccagcacc
    12481 gcgccgggga actgcgtttt cagggcggcg agaatcgctt cgccacgatt tgacgatgaa
    12541 ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct gaaggcaaaa aatcatgcca
    12601 ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg actatagtgg aagcgcccgc
    12661 caggatcctg atgacaggtt gtggaatatg cgttcccatc gccagcatca tgaccagcag
    12721 aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc cggttaacgg cctgcggcgg
    12781 tttcgccatt aacacccgcg ctgccatccg taccaggccc gccagcacca gcgttaacag
    12841 caataacagc aggacgataa tcagcaggtg attacgtgcc agtccggcgg taatggtcat
    12901 aaattcgcta agaaaaatgt tgaagggcgg catccctgcc agcgccagcg ccagcgcacc
    12961 gccgccaaac agcacggcgg taaatggcat gattttgagc atcccacaga cgacgttgag
    13021 atcgcgcgtg ccgtacttga gcagtacatt gccggaaccg cagaacagca gcgtttttgc
    13081 cagactgtgg tttaaggtgt gcagcagcgc ggcaaaaatt cccagtggcc cgccaatgcc
    13141 tagcgccacc gcgaccagtc ccatgttctc cacgctggag tacgccagca gacgcttaat
    13201 gtcccgctgt accagaatga aaaatgccgc cacggcaacc gacaacatgc cgaagatgag
    13261 caacaaccgg ttggggaaat cgctgccgat ggcctggcag ataatgatgt aatagcgaat
    13321 tagcaccaac agcgcgcagt tcagcaatac ggcggagagc agggcgctga ccggactcgg
    13381 tgcttcactg tgagcatccg gcagccaggc gtgcatggga aatagcccgg ttttggtgcc
    13441 aaagccaatt agcacaaaca caaaggccaa cagcattaat gttgggtcaa gcaaggacga
    13501 ttgcttaaga acctcgctcc agaatatcgc catttctgcc tgcggcataa cgctggcgac
    13561 gttggcgtat accagcacgg taccgaacag accaaaagcg acaccaacag tacaaataat
    13621 gatgtacttc catgctgctt ccagcgatga acgctgaccg taaatgccta ccagaaacgc
    13681 cgagcttaag gtggtggctt cgatcgccgc ccacatcaca atcaggttat tgctggtaac
    13741 aaccagcagc atggtaaact aaaacagatg gaagaaaccg tagtaatcgc acagcgtaac
    13801 gggggaaagc tcgccgtgtg ccacttcatg acgcatgtag ccaatcgagt aaacaccggt
    13861 gagaaagcca atcacaccaa gaatggcgag gaacaaaccg ccagaccatc aatatgaagc
    13921 cacagtcccg cagcgaatat ttctcctgca tcagcggcag tttggaccac ccagagtgcc
    13981 agaatcagca gcagtgtgat ccctaagcta tgtaatacag tcaccgtaca agtcgcagag
    14041 agcccccgtt tccggcaggc aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt
    14101 aaagcgaaca tcacagaata actcatctca ttatcccttc agcgcggtca agttgttcac
    14161 gtccagcgtg ccgtgggtac gccatatttt tcttgccagt aacaccatca caatgacggc
    14221 gaagatggcg tcggtagcga tacctatttc caccagttcc ggtgctcgcc aggcaagaag
    14281 cgccagcacc agatgggagc cgttttccat caggcagtaa ccaaaaattt gccgcaggat
    14341 attgcgctgg ctgacaatgc acagcaggcc aagcagaaaa tgacctaacg ctaccgccag
    14401 cgccggtttc agcccggtag ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag
    14461 caggacaatt aacgccgcga gcagagccat cattgccgga ccgaataacg ctttttccgg
    14521 gatgttctgg ggaatatttc gtgcagcgta agtcatgatt aacggtacca gcagcacttt
    14581 ggtgataaag gcactggcgg accagatcag cagttgctct gcggcgaaca ggcacgagag
    14641 agtggcgaaa atagacacca gcaccagcga ctggcaggcg taaaatccgc atgacaggcg
    14701 atagcttttg acgctaatca caaacagcga tgtcagcatc atcagtcccg ccagattatt
    14761 tacgatcata gaaccggtca ttcatcatct ccttactgca accaggtcgc ggcgattacg
    14821 ctggaaatca gcgacatcac aatcagtaca atcaacacca ggcgcattga ccctggcagc
    14881 ggtgcggcat cggcgacggc ctcgctcggt ttgccaggca caacgcgacc aaaccagcga
    14941 ataaaccagg cgaaactggc gaccgattca atcatcagca gaatcatggc gggcagcagg
    15001 atccagtact ccactgacaa cgcaaaaccg gcagcaaaca gcgggaattt actgaagaag
    15061 ccgttgaacg gcggcacgcc ggtaatcgcc agcgctgcca cgcagaaacc cacgcctggc
    15121 aacggcaggg tgtgcaatac gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa
    15181 ctcagcgcac ctgctacaag gaaaaacagg cttttagcga acgcgtggtt gacgatgtag
    15241 gcgatgctgc cctccagtgc cagccgccca ctttcaccat cgatgcggcg tggagatagg
    15301 cgctgatcgg tgttggcgct tccattgcgt ccggtagcca cgcttgcatc ggtagctggg
    15361 ccgatttccc ccacgcggca aacaggatgc cgccataaac cagataacgt gcgtcgccat
    15421 gtaactcgct catcgcgcta agcacaaacg ttccggtctg caaaaacagc gtggcggcgg
    15481 caagatacaa ccccaacgaa ccgatatggg tgataagcaa cgcttttagt gctgaacgct
    15541 gcgctttatc gctctggtaa taactgatca acgcccagga gcagccgccc gtaatttcaa
    15601 aaaacaacaa ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa
    15661 acaacagcaa aatgcgtaat aacgattcgt gccgttatgc gggtgttcgc gatttttatc
    15721 cgtcagataa cccgtggagt agatcgtgac cagcaaaccg agaaacacca ccacaaacag
    15781 aatcagcgta ctcacgcgat caatgaccag gccaaacaat gccacatccc ccacgttgac
    15841 caacgtcaac gtgacggcaa ctttatcggc ctgataaaac gcggagatca gcgacaacat
    15901 gcacagcgtg gtcagtgcgg cgaacaaaac ccccattcgg cggcccgacg ttgtggcgaa
    15961 aacgaaacga ccagtgcgcc gataaaaggc agcattaacg tcgtcagagc aagattttcc
    16021 atattccgtc agtgctcctt acagaccggt taaccagaag acccatgcaa gcgcagcaag
    16081 gctgaagcca agccaggtca catggtgaat gagtaaaaaa cgcccgcgtg ccagcgtgtt
    16141 ttctgcgatt gaggccagta caaaaatcag caaaaccttg agcagcgtaa cgacaagtga
    16201 agtcagcagg caggcgagag aaagttcttg cgcgcgccca aagggcagaa acagggccac
    16261 aaacagtgct gccatcacga cctgtttcag ccccagcccc catttcgcta gcgccagccc
    16321 ggcaccggaa tattcggtca gcgggccttc ctgtaattcc tgttctgctt cagcaacatc
    16381 aaagggaatt tttcccatct caatgaagca ggcaaaacca caggccagta acgccagtac
    16441 ggtggttagc ggcgagttcc agcccgttgc cagcgtattg ctgatcatct cgatatgcgt
    16501 ggaacctgct atcagcgcca ataccagcag tgagagaatg agcatgggtt cgaccagaat
    16561 gccgagcgtc aactcgcgac tggcaccgac tcccgcaaac gggcttccgg tatccagccc
    16621 ggaaagagca aagaaaaaac gaaacagggc aagaagatag ataagggtga tcagatcgcc
    16681 gccgcccgca aaaggggaaa cggtaataaa aagtggtagg gccatcgcca gcaccagcat
    16741 gctgctgatt aatacccacg gcatcaggcg gaacatcaga cctgaagata taggcgcaac
    16801 ttcctggcgt ttaaacagtt tgtggatatc gcgataatcc tgccagatcc ccggcccgcg
    16861 gcgggagtgc atacgcgcgc gtatctgccg ggaaataccc gtaaaaagcg gggttagcat
    16921 cagcagaatc acggcctgtg ctaacgcaaa aatgaccaga tatccgtcgt aaagagtttg
    16981 tctcatggtg atttccttag acggcaatag cgattagcag cacaaccagt gcggcgacca
    17041 catacaggca atagagacga aagtcgccgc tttgcagatg ctgtatttct ttggccagcc
    17101 gttgggtggc gctcacgatg gggcggatca cccgctcatc ccagaagggt tctgtgctct
    17161 gagccctggc ggtgacgtgc gcaaaacctt tatttagcct cagcgtaggg tcgagctgtt
    17221 gtcgtagacg aaataacgca gagaagacca cacgcagcgg ctgcatcacg ccattgcctg
    17281 acggggccat cgcattttcc cagccgtagc cgcatgccca ggcatctcct gtgcgacgaa
    17341 acgctgcgcg gcgcgaacga caccacagcc agtaaaggcc aggcagcaaa ggcatcgcca
    17401 gtagtaacag caacagtaat gagggggtga cctgtgtatg aaacgtgccg ggtacaagtg
    17461 ctattccgct ggcgacagtg acgggagggg tattggtaaa cgcatgggca atatgcatta
    17521 tcttcggtgc cagccaactg gcactaagcg caattaatac gcagagtgcc gcgagcagta
    17581 gcatggcgac gatcatcgta tttggcactt cctgtgcctc ttcagcgtgt gtactgcgcg
    17641 gcgcaccaca gaaagtaata ccgtacattt ttacgaagca cattactgcc agcccaccag
    17701 tgactgccag cattaccata gcaataggac ccgcaagttg tagcgctacg gcttccacac
    17761 gacttagtga gaacagcgac tgccaggtgt accattcgct gataaaacca ttcagcggag
    17821 gaatggctga tatcgcgagg caaccaatca ggcatgctgc ggctgtccac ggcatccgtt
    17881 tcgccagtgc ccccattttt tccatgtcgt gggtatgcaa acgcgaaata atcgcgcccg
    17941 cgccgagaaa tagcagccct ttgaacagcg catggttgag cagatgaaac agtgcgccga
    18001 gcagtccaac cacggtgagc agcgggtcgt gcagtgacag accgaccatc gccacaccga
    18061 ctgccagcaa aataatgccg acgttttcga cggtactcca ggccagcagc cgtttgatat
    18121 cctgttccgc cagtgcatac agcacgccca ggagcgcgga gattgcgccg atcgccatca
    18181 ccagagtgcc ccaccacaga ggcaaacccg tttgcgccag cagatccatc gctactttca
    18241 ggatgccgaa aataccgatt ttgaccatta cgccagacat caacgccgaa gcgtgcgatg
    18301 gtgcggcagg gtgagcgcgc ggcaaccagc tgtgcaacgg catcatcccg gctttcgcgc
    18361 caaaaccgaa aaaggccagc agaaacaccg ccgacgccag ccccggagaa agtgaaagcg
    18421 tgcgaaactg gcaaaatcga ggctgccgct ttcgcgcccc atcagcaaga aggcgatcat
    18481 aatcagcacc gatccggcgt gggcgataaa aaagtagagc atgccagcgt tgatcgacgt
    18541 tttatcctgc ctggcaatga ccagaaacca ggaagacagc gacatcattt caaacagcac
    18601 gatgaaccaa aaagcgttgt ccatcaccag cagggcaacc atcgatgcga tgaaaagatt
    18661 cataaagaag cccatcgccg ccgcgccttt gccctcgtat tcgcgcatat aagtcaatga
    18721 atagagcgaa caaaccacca ccagcaagga gataaccagc accataaatg ccgagaggct
    18781 atccattcgc agggttaaat cggcaaagtc aaaaggggtg gcaaaatggg cgactaaagt
    18841 tacaccagca tgtaattgcg taattccgct gatgatgcca atcacgccgc ccaccagcga
    18901 tgtgatgccg gaaagcttaa tagccagtct gtccagaccg agtaaaaaca gcgaagccag
    18961 actggcaaac agatagagga tcagcgacca ggttaataat tgcagggcat ccattagtgt
    19021 tgctcctgag tgagggaaag gggatcctcc ccgccattgg caaaccagct tgcggcaagg
    19081 cgctgttttt ctttcatctg acgttgcagg ctatcgtcgg tgatcagccg taacgcctga
    19141 ttcgggcaag cgcgaacgca ggccggacct tctggcaaga aatcacacag gtcgcatttc
    19201 accgcgacgg tctggacacc cggttcccag cgcagcaacg catgttgggg cagcacgttt
    19261 tcttcgccgt ctttgagtga gccttcagcc tgaaaaacat attgcgcatg ggcattcacc
    19321 ggacggcttc ctgaagcgct gattgcgcca aatgggcaga ccacggcgca aagcttgcag
    19381 ccaatacaga ggctttcgtt gagttggatc gcatcatccc tctgagagat ggcattgacc
    19441 gggcagacct gcaggcaagg ggcttcctca cagtgatgac acacgacagg ggcagtgatt
    19501 gttgacgtct tcgccagggc caggcgcggg tgttgctgta aaccttgcgt tttatgcacg
    19561 tccgaacagg cagcgagaca ggtattacat cctgtacacc acagtggttc ggccaccaca
    19621 aagcggttca tgtcggctcc ttgaaaatga tatgaaacat tgcatttcat attgcataaa
    19681 ggatgccatt ctgattaact caatatattc aataagttat gtattgactg gattctgaac
    19741 ggtacaaaaa tgatggtcat taacgttcag caaaaaatgg attcgacacc cgtgtcgcag
    19801 gggaaagcga gaatgtgatc ttgatcacga gaagtgaggt atgctggccg caagcgcagc
    19861 cagcacggaa tggagcaaag taatcaggcg tgttctttca gccagttcag cacaacgtcg
    19921 tggtgattgc tggttttgaa atcgtcaaag acatgttcga ttttgccatc agcgtcaatc
    19981 aggaagctga tgcgatgaat gccatcgtag gttttgccca tgaaggactt ttcaccccag
    20041 acgccgaatt gttcgcacac ctggtggtcc tcatcagaca ggagcgtaaa gttaagcagc
    20101 tctttttccg caaaacggga gagtttttcg ggtttatcgg tgctgatacc cagcacatca
    20161 acgcccgctt ttttcaactc atccatgtta tcgcgtaagc cgcaggcctg tacggtacag
    20221 ccgggggtca tggctttcgg gtagaaataa accagaacac gctgtccctg gaagtcggtc
    20281 aaattaactt gttctccgtc ttgatccggc aagctaaatt tcggtgcgat atcaccggct
    20341 ttcagtggat tcattactta actccatcct gttcatcatg ttgggaataa ttgacgacgt
    20401 taatactgcc ttgtgcattg agttctgtac atagggcttt gaacgcttgc tcaatatttg
    20461 ctgcgtccgc agatgcgggg ctgtgggcgg ttatctgaat atgcaactgc gcagcccgtt
    20521 cattttcagc aggttgcgtg cgcgacacca gctccgcaat gttcatatga tgcgcgtcga
    20581 aaagtgctgt gaagcgttca attaaatgcg gggagtctgc cacatcgacc tgaacccaga
    20641 cagatgctgg cattggcgga cgcggacgcg ccgtcgtgcg cttcatcacg attaaaagat
    20701 ccagttcggc acctttcaac ggtaacgttg attcaatcag agtaatggca ttccatgaac
    20761 cggaaagcag cataataaac gtgaactctt ctcccagcat cgccaggcga ctgtcttcaa
    20821 tattacagcc gcaactactg acatgacggg tgatggtgtt cacaattcca gggcgatcgg
    20881 cacccaacgc agtgatcacc agataatgtt gcgatgacag tgtcaaactg gttattcctt
    20941 taaggggtga gttgttctta aggaaagcat aaaaaaaaca tgcatacaac aatcagaacg
    21001 gttctgtctg cttgctttta atgccatacc aaacgtacca ttgagacact tgtttgcaca
    21061 gaggatggcc catgttcacg ggaagtattg tcgcgattgt tactccgatg gatgaaaaag
    21121 gtaatgtctg tcgggctagc ttgaaaaaac tgattgatta tcatgtcgcc agcggtactt
    21181 cggcgatcgt ttctgttggc accactggcg agtccgctac cttaaatcat gacgaacatg
    21241 ctgatgtggt gatgatgacg ctggagctgg ctgatgggcg cattccggta attgccggga
    21301 ccggcgctaa cgctactgcg gaagccatta gcctgacgca gcgcttcaat gacagtggta
    21361 tcgtcggctg cctgacggta accccttact acaatcgtcc gtcgcaagaa ggtttgtatc
    21421 agcatttcaa agccatcgct gagcatactg acctgccgca aattctgtat aatgtgccgt
    21481 cccgtactgg ctgcgatctg ctcccggaaa cggtgggccg tctggcgaaa gtaaaaaata
    21541 ttatcggaat caaagaggca acagggaact taacgcgtgt aaaccagatc aaagagctgg
    21601 tttcagatga ttttgttctg ctgagcggcg atgatgcgag cgcgctggac ttcatgcaat
    21661 tgggcggtca tggggttatt tccgttacgg ctaacgtcgc agcgcgtgat atggcccaga
    21721 tgtgcaaact ggcagcagaa gggcattttg ccgaggcacg cgttattaat cagcgtctga
    21781 tgccattaca caacaaacta tttgtcgaac ccaatccaat cccggtgaaa tgggcatgta
    21841 aggaactggg tcttgtggcg accgatacgc tgcgcctgcc aatgacacca atcaccgaca
    21901 gtggtcgtga gacggtcaga gcggcgctta agcatgccgg tttgctgtaa agtttaggga
    21961 gatttgatgg cttactctgt tcaaaagtcg cgcctggcaa aggttgcggg tgtttcgctt
    22021 gttttattac tcgctgcctg tagttctgac tcacgctata agcgtcaggt cagtggtgat
    22081 gaagcctacc tggaagcggc accgcttgcg gagcttcatg ccccggctgg aatgattttg
    22141 ccggtgacct ccggtgatta tgcaatcccg gtgaccaacg gtagtggtgc tgtcggtaag
    22201 gcgctggaca ttcgtccacc agcccagccg ctggcactgg tttctggcgc gcgtacccag
    22261 ttcacgggcg ataccgcttc attgctggtg gaaaatggtc gtggcaatac tctgtggccg
    22321 caggtggtta gcgtgctgca ggcgaaaaac tacaccatca cccaacgtga tgatgctggt
    22381 cagacactga ccaccgattg ggtacaatgg aaccgtctgg acgaagacga gcagtatcgt
    22441 ggtcgttatc aaatctctgt taagccgcag ggttatcagc aggcggttac ggttaaactg
    22501 ctgaacctgg aacaggcggg caaaccggtt gcagacgcgg cttccatgca gcgttacagc
    22561 acggagatga tgaacgttat ttccgccggt ctggataaat ctgccactga cgccgcgaac
    22621 gctgcgcaaa atcgtgcctc caccactatg gacgtacaaa gtgcagctga tgacaccggt
    22681 ttaccgatgc tggtcgtacg cgggccgttc aatgtggttt ggcaacgtct gccagcggcg
    22741 ctggaaaaag tgggcatgaa agtgaccgac agcacccgtt cgcagggtaa catggccgta
    22801 acttataagc cgctgtctga cagcgactgg caggaactgg gcgcgagcga tccaggcctg
    22861 gcatccggtg actataaact gcaggttggc gatttagata accgcagcag cctacagttc
    22921 atcgatccga aaggtcatac tctgactcag agtcagaacg acgcgctggt atctgtcttc
    22981 caggctgcgt ttagcaagta aaaatacagg gctggaatca tccggccctt ttttctgata
    23041 tgatacgcaa acgtgtgcgt cggcaggaaa acgcgatttt agcggtaatt cgcacgaaat
    23101 ttgtttgtcg gacgtagttc ggataaggcg ttcacgccgc atccgacaaa acatccggca
    23161 caccagacag caaaagattt taaaacgtta attcacaccc aggagtgata aagatgcaaa
    23221 agcaagctga gttgtatcgt ggtaaagcga aaaccgtata cagcacggaa aacccggacc
    23281 tgttggtgct cgaattccgc aatgatacgt cagcagggga tggcgcgcgc attgagcagt
    23341 ttgatcgcaa aggtatggtg aacaacaagt tcaattactt cattatgagc aaactggctg
    23401 aagcgggtat cccgactcaa atggagcgtc tgctctccga taccgaatgt ctggtgaaaa
    23461 agctggatat ggtgccggtt gagtgtgtcg tgcgtaaccg tgctgctggc tctctggtga
    23521 aacgtcttgg aatcgaagaa ggtattgagc tgaacccgcc gctgttcgat ctgttcctga
    23581 aaaacgacgc catgcacgat ccgatggtca acgaatctta ctgcgaaacc tttggctggg
    23641 tgagcaaaga gaacctggcg cgtatgaaag agctgaccta caaagcgaac gacgtgctga
    23701 aaaaactgtt cgatgatgct ggtctgattc tggtcgactt caagctggag tttggcctgt
    23761 acaaaggtga agtggtactg ggcgatgagt tctccccgga cggcagccgc ctgtgggaca
    23821 aagaaacact ggagaaaatg gacaaagacc gtttccgcca gagcctcggt ggcctgatcg
    23881 aagcctatga agccgtcgcc cgccgcctgg gtgtacagct ggactgattt ttctggttgc
    23941 ttgtttcttt agccggaggg tactccggct aaatgtcttt gatacccccg ctacaatact
    24001 tctcattatc tccctttgac aggaaagtca taccaggcca atatgtatat acataacgta
    24061 tatcccaaaa agggggggct atggaaacta ctgtattttt aagtaatcgc agtcaggccg
    24121 tcagattacc gaaagcggtc gctctgccag aagacgttaa gaaagtagaa attattgcga
    24181 ttggtcgtac aagaatcatc actcccgccg gtgagtcctg ggacagttgg tttgatggcg
    24241 aaaatgtgag tgcggatttt atggatatcc gtgatcaacc cgcaatgcag gaaagggaat
    24301 cattttgatg ctgaaattca tgctggatac caatatctgt attttcacta ttaagaataa
    24361 accagtacat gtcagagagc ggtttaacct gaattctggc cggatgtgta taagttctgt
    24421 aacattgatg gagcttatct atggggcaga aaaaagtcag atgccagaac gcaatttggc
    24481 ggttattgaa ggtttcgtat ctcgtttggt agtgcttgat tatgatagtg ctgctgcgac
    24541 gcataccggg caaatcaggg ccgagcttgc ccggcaggga aggcctgttg gaccttttga
    24601 ccagatgata gcgggacatg ctcgcagtcg cggtttgatt gtggttacca acaatacccg
    24661 agagtttgag cgagtggcgg gaatcagact agaagactgg agctaacatt ctcttttact
    24721 tctaacattc gtaaattata taacgtggca tgaagcctta attcctccgc catttcatcc
    24781 cgctttcctg tggtaatctt cctctgaacc acctacgatc atttctataa tgaatatatt
    24841 gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga agacaggcgc
    24901 aatagctccg gcggtccgtc aatgggcggt cccggtttcc gtctgccgag cggtaaaggc
    24961 gggctgattt tactgatagt cgtgctggtt gcaggctact atggcgttga tttaaccggg
    25021 ttgatgaccg ggcagccggt ttctcaacag caatcaacgc ggtcaattag cccaaatgaa
    25081 gacgaagccg caaaattcac ctcggtgatt ctggcaacca cggaagacac ctggggacaa
    25141 cagttcgaga agatgggtaa gacctatcag caaccgaagc tggtcatgta ccgtggaatg
    25201 acgcgtaccg gatgcggggc gggccagtcc ataatggggc cgttctattg cccggcggat
    25261 ggcacggttt atatcgatct ctccttctat gatgacatga aagacaaact tggcgcggat
    25321 ggcgattttg cccaggggta cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg
    25381 ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac
    25441 cgcttatctg tgcgtatgga actccaggcc gactgttttg ccggtgtctg ggggcatagt
    25501 atgcagcagc aaggcgttct ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag
    25561 gccatcggcg atgaccgttt acaacagcaa agtcaggggc gagtagtacc agacagtttc
    25621 actcatggca cttctcagca acgctacagc tggtttaaac gtggtttcga cagcggcgat
    25681 ccggcacaat gcaatacttt tggtaaaagc atttaacttc cggggcaggg atggctgaac
    25741 tgactgcgtt tcacacatta acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg
    25801 tgttgagcgg ggaagagggt tggtgttttg atcatgcgct taagttgcgt gatgccttac
    25861 ctggcgactg gctgtggatt tcgccgcagc cagatgctga aaaccactgt tctccctcgg
    25921 cactacaaac tttacttggg cgcgagttcc ggcatgcggt attcgacgcc cgccacggct
    25981 ttgatgccgc tgcctttgcg gcacttagcg gaacgttgaa agcgggaagc tggctggttt
    26041 tgttactccc tgtatgggaa gagtgggaaa accaacctga taccgactcg ctgcgctgga
    26101 gtgattgccc tgaccctatt gcggcgccgc attttgtcca gcatttcaaa cgtgtactta
    26161 cggcggataa cgacgctatt ctctggcggc aaaaccagcc gttctcgttg gcgcatttta
    26221 ctccccgtac tgactggcac accgcgaccg gcgcaccaca accagaacaa cagcaactct
    26281 tacagcagct actgaccatg ccgtcgggcg tggcagcggt aacggctgcg cgtgggcgcg
    26341 gtaaatcggc gctggcaggg caactcattt ctcgtattgc gggtagtgcg attgtcactg
    26401 cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc gggcgagaag tttcgcttta
    26461 ttgcgccgga tgccttgtta gccagcgatg agcaagccga ctggctggtg gtcgatgaag
    26521 ccgcagccat acctgcgccg ttgttgcatc aactggtatc gcgttttcct cgaacgttgt
    26581 taaccactac ggtgcagggc tacgaaggca ccggacgtgg ttttttgctg aaattttgcg
    26641 ctcgctttcc gcatttacac cgttttgaac tgcaacagcc gatccgctgg gcacagggat
    26701 gcccgctgga aaaaatggtt agtgaggcac tggtttttga cgatgaaaac ttcacccata
    26761 caccacaagg caatatcgtc atttccgcat ttgaacaaac gttatggcga agcgagccag
    26821 aaacgccgtt aaaggtttat cagttattgt ctggtgcgca ctaccggact tcgccgctgg
    26881 atttacgccg gatgatggat gcaccagggc aatatttttt acaggcggct ggcgaaaacg
    26941 agattgccgg agcgctgtgg ctggtggatg aggggggatt atctcaacaa ctcagtcagg
    27001 cggtatgggc aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc
    27061 acggtagcaa tccactggca gcgacattgc gtggacggcg ggtcagccgg atagcagttc
    27121 atccggcgcg tcagcgggaa ggcacagggc agcaacttat tgctggtgct ttgcaatata
    27181 cgcgtgacct cgactatctt tcggtgagtt ttggttacac cggggagtta tggcgtttct
    27241 ggcaccgctg cggttttgtg ctggtgcgga tgggtaatca tcgtgaagcc agcagcggtt
    27301 gctatacggc gatggcgctg ttaccgatga gtaatgcggg taaacaactg gctgaacgtg
    27361 agcattaccg tttacgtcgc gatgcgcaag ctctcgcaaa gtggaatggc gaaacgcttc
    27421 ctgttgatcc actaaacgat gccgtccttt ctgacgacga ctggcttgaa ctggccggtt
    27481 ttgctttcgc tcatcgtccg ctattaacat cgttaggttg cttattgcgt ctgctacaaa
    27541 ccagtgaact ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgtc agcgacgcgc
    27601 agttatgtac cacacttaaa ctttcaggcc gcaagatgtt actggtccgt cagcgggaag
    27661 aggccgcgca ggcgctgttc gcacttaatg atgttcgcac tgagcgtctg cgcgatcgca
    27721 taacgcaatg gcaatttttt cactgactcc ttcaattttc tcccatggtc attgtgagtc
    27781 agcggcgtaa aattaatgac atcagttaag gagttcgcca tgaaacatga ccattttgtt
    27841 gttcaaagcc cggataaacc tgcgcaacag ttgttactgc ttttccacgg tgtcggggat
    27901 aacccggtgg caatggggga aataggtagc tggtttgcac cgctgtttcc tgatgcgctg
    27961 gtggtgagtg tcggtggcgc ggagccgagc ggtaatccgg cggggcgtca gtggttttca
    28021 gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg atgccatcat gccgacgttt
    28081 attgagacgg tgcgctactg gcagaaacag agcggagtag gggctaacgc cacggcgtta
    28141 atcggtttct ctcaaggcgc gattatggcg ctggagagca ttaaagccga accgggcctt
    28201 gcttcacgcg tcattgcttt taacggacgc tatgcgagcc tgccggaaac tgcgtctaca
    28261 gccacgacaa ttcacctgat tcatggcggt gaagatccgg ttatcgatct ggcgcatgcg
    28321 gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag
    28381 gatttaggcc atgcgatcga caatcgcagt atgcaatttg ccctggatca cttgcgttat
    28441 accattccga aacattattt cgacgaagcg ctaagtggcg gtaagcctgg cgatgatgat
    28501 gtgattgaaa tgatgtaata atagccgggt aggcctgata agactgtatc aggcatcatg
    28561 gccggatgtg gcgtgaacgc cttatccggc cactcagctt acttcttcgg ttgatccttt
    28621 ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg gggaagctcc
    28681 ggcttgttgg cgagaaattt cttatggtcg acgcgcttga gatctttaat cacgttcagc
    28741 aggacgccta ccaaaaagac aatcaccaga atccaccaat atttagccag ccagtccatg
    28801 cttatttcct cttgcagaac cactcatcac gcgacgagct gttccatgat acgttgatac
    28861 atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt atgaatagtg
    28921 gcattgaccg gcccgagttc caccacctgc gcccccatgc gggcaataaa gcgcccgtcg
    28981 gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc aaccgcgtta
    29041 acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga aagccaccag
    29101 tccaccgtat aacgcagttg atgtttttca agcagggcaa gcacctgcgc tttgatcatc
    29161 tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc acccggaata
    29221 acgttgttac tgcccgttcc cgcctgaata ttagcaatct gcatactggt cgccgggaaa
    29281 aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg tgctgcgcga
    29341 tgtactggat tgtcagccag atgcgggtag gccacatgcc cctgaacgcc atgaatggta
    29401 aggttgcagg ttaacgatcc gcgacgacca tttttcacca catcaccaac cacttcgata
    29461 ctcgacggtt cgccgaccag gcagtaatcg agacgctcat tacgcgacat taacgcttcg
    29521 acgactttta ccgtaccgtt gtgggcacta gcttcttcat cagaagtgat cagaaatgcc
    29581 agtcgccccg tatggttggg atgttgtgcg acaaaacgtt ctgccgctac caccatcgcc
    29641 gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccgtc acgaatggtt
    29701 ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc
    29761 ccggcaaagg ctaacgtttc accctgccca cgccatgccc aaaaattctg cgtatcggca
    29821 aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg
    29881 catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt cagctcaata
    29941 accgggcacg acatagacta cacctcatgg aaaaattgct gataactgga atcactgaaa
    30001 cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat tgcaggcatt
    30061 tcagtcatta atgccgccgc agaggccgca tcggtgattt tattgcgggt ggtttcgtcc
    30121 agtttacgcc aggttgtacc acgggtgttg agtaacgctt cccagcctaa ttcgttgata
    30181 aaacccttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa acgatagtcg
    30241 atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt tttgatgccg
    30301 taaagtgtaa ccataatgaa tcctttttcg atttatgaga ataaatactc atttaagggt
    30361 gaaaacattt tgcctgcaaa tgattatcag gaaatataac attatcgaag tattcaaatt
    30421 aacaactatt aaataatatt aaacattttt aacgtaaaca ttaagaatat tcatcttgtt
    30481 atctgtctgt attttaacca gaattaaatc agtattagat gccgtatgaa tatctcgaaa
    30541 tgttgctgtg tatcacatac aaattatcac cgcaggacca tagtatttac ataaggtttg
    30601 tcccccggag gtagtcatcg cagcaatcga gtaaattagc ttcaccacgg tatattgttt
    30661 ccgtagaata cccataatta taagagaggt tgttatgatt gaacgtgaac tggggaactg
    30721 gaaagacttt atcgaagtta tgcttcgtaa gtaattcctg agacaaagta caaacagcaa
    30781 gaacccgctg agcaggttct taatcggaaa gttgaaaaag gcgacacatt agcatgtcgc
    30841 cttttttatt gcttattccg ggcgcggctt cagcgggaag cggcggcgca ccagtacaaa
    30901 gaacagcggc acgaagtaaa tagccagaat agtggccgaa atcatcccgc ccattacgcc
    30961 agtacccacc gcatgctgac caccggaacc ggcacccgtg ctggttgcca ttggcaatac
    31021 gccgaagata aatgccagcg aggtcatcag aatcgggcgt aaacgctgac ggcaggcgtg
    31081 gagcgtcgct tcaaacaggt cgtggccttt ttggttcatc tcgttagcaa actcgacgat
    31141 caggatggcg tttttcgccg ataaaccaat gaccgttaac aggcccacct ggaagtaaac
    31201 gtcgttttcc agcccgcgca tccaggttgc cagcagcgcg ccgataaccc ccagcggcac
    31261 gaccagcatt accgagaacg gcaccgacca gctttcatac aacgccgcca gacacaggaa
    31321 taccaccagc aaggaaatag cgtacagcgc cggagcctgc gcgccggaaa gccgctcctg
    31381 atacgacatc gccgtccact ccagaccaaa gccgtttggc aactgcttca ctaacgattc
    31441 cataatatcc atcgccgtac cggtactgac ccccggtgcg gcttccccaa caatctcaac
    31501 cgcagaataa ccgttatagc gttccagacg cggcgagcct gtctcccagc gtgaggtcgc
    31561 gaaagcagag aagggcacca tgccgccatc tttatttcgg acataccaga gattgatgtc
    31621 atctggcagc atgcgatacg gcgcagctgc ctgcacatag actttcttca cgcgaccgcg
    31681 atccataaag tcattaacat agctcgaacc ccaggcggtt tgcagtgtgt cgttaatatc
    31741 gtcgatatca acgcccagcg cctgagcttt acgctggtcg atatcaatct gcaactgcgg
    31801 actgtcgtcg aggccgttat ggcgcacacg ggttagctcc gggttttccg ccgccagcgc
    31861 cagcaactgg ttacgtgctg ccatcagcgc atcgtgacct gctccagcgt ggtcctgcaa
    31921 ctccatatca aaacccgcag aactaccaag accgctaatt gctggcgggc tgctggcgat
    31981 aacgcgagct tctttaatct tgttaaacgc ctttgttgca cgctcgataa tggcaaacga
    32041 ggtgccggtc ttactgtcgc gttcgctcca gtctttcagg cggataaaca ttcgcgccac
    32101 gttttgcccg ttacccccgg ggccagaacc aacagtggca aacaccgaca tgatgttgtc
    32161 tttttcatgg gtgaagtaat atttctcgat ttgctcaacg actttcaggg tctgttgttg
    32221 ggttgaaccg ctgggcaatt gtaccgaggt agtaaacatg ccacggtctt ccagcggtaa
    32281 gaacgacgtc ggcaaacgca ggaacaggaa caccatgccg ccaagcagca ggacataaat
    32341 cacaatccag cgcaggctac ggtggagaat tttcgccacc cctttttcgt agcgttcggc
    32401 gttgcggtta aacatctggt taaaccaggc aaaaaagcct ttttgcccgt gatgctcacc
    32461 ttttttcagt ggcttaagta gtgtggcaca cagagccgga gtgaggatca tcgctaccag
    32521 tactgacagc accatcgccg caacaatggt aatagagaac tggcgataga tggcaccggt
    32581 ggtgccgccg aagaaggcca ttggtacaaa taccgccgaa agcaccatcg caatcccgac
    32641 cagtgccccc tggatctgcc ccatcgattt acgtgtggct tcgcgaggag tgagtccttc
    32701 ctcactcata atacgttcga cgttttccac caccacgatg gcgtcatcca ccagcagacc
    32761 gatcgccagc accatcgcga acatggttaa ggtgttgacg ctgtaaccaa aggcgtaaag
    32821 tacggagaag gttcccatca acaccaccgg cacggcgata gtgggtatca gcgtggcgcg
    32881 gaagttttgc aggaacagat acataacgag gaaaaccaga gcgatagctt ccagcagcgt
    32941 tttcaccacg tcttcaatgg aggctttaac aaacgaggtg gtttcatacg ccaccttgta
    33001 ttccagtccg tgcgggaaat actgcgccag ctcgtcgaga cgattgagca ccagctccgc
    33061 tgtcgccatt tcgtttgcgc cggaggccag ttttaccccc agcccggagg ctggcttacc
    33121 attgaagcgg ctaagataat cgtatttctc cgcccccatt tcgacggtgg cgacatcgcc
    33181 cagccttacc tctgagccgt cttgattcac cctcaaggtg atatcgcgga actgttctgg
    33241 tgtttgcagc agtgactggg cgttaatggt ggcgttgagc gcctgcttat cgacggaagg
    33301 tgtaccacca agttgcccaa ccgcaatctg cgcgttctgt gactcaatgg catcagtgac
    33361 atctttagcc gtcatctgga aactgttgag tttcgccggg tccagccaga tacgcatgga
    33421 atattgcgaa ccataggcat cgatatcccc gacgccattc acgcggctta acgggtcctg
    33481 aatattactg gcaacataat cggcaatatc ctgtttatcc atcgaaccat cggtagagac
    33541 gaaagcaatg gtcagaatat tggtatcgcc ggttttacgc accgtcacgc cctgattttg
    33601 caccgcctgc ggtaactttc gcatggctga ttgcagctgg ttttgtactt gctgcacggc
    33661 ttcgtccgga tcggtgcctg ctttaaaact taaagtgaca gatgcctgac cggtgccact
    33721 gctctgagat gacatataca tgagattatc gaggccggtc atattttgct cgataacctg
    33781 ggtcacggtg ttttccagcg tctgggccga tgcgccggga tagttagcgg tcactcgcac
    33841 attcggtggc gcgagatcgg ggtattgttc aacgggcaat gaaaaaatcg ccagggtccc
    33901 tgtcagacac aacaggattg ccagcaccca ggcaaaaatg gggcgatcaa taaagaaatt
    33961 cgccattaaa agaggacctc gtgtttctgc gtatcgttgt aggcttgact tgcttcactg
    34021 tagcggcaag gtacgagcca atcgtggaga aaaaaaggag ttaatgtaaa ttatcatgcc
    34081 cttttatact gaaccttaag tgcaagtatt ctttggtcag taggaggcat tacatcaact
    34141 gactttcctc accctccgca gagcgaaagc taatactcac taacgtgccg ccgccggaag
    34201 gttgtgaaaa agtcagcgtc ccacccagcc gttccgcgcg ttcgcgcatg atattcagac
    34261 cataatgacc ttcgggttct ttcggttcac cgataccaat cccgttatca cggatataaa
    34321 ccgtgtgatt gccgtccggc gcggtgacgc aactgacggc gatttcgctg gcgttggcgt
    34381 gcttcatcgc attcagcacc gcttcgcgaa taatttgcaa caaatgcacc tgcatttgcg
    34441 catccagcgc cagggttggc agacggcagt cgagggtcag tttggcgctg gtttgatttt
    34501 gtaacgtatc cagcatttcc ctcaacgcgg aggggagatc cgcctgctgc agcgtcaggc
    34561 ggaaagtagt caacagctcg cgtaactgcc gataagcatc attcaacgcc tgggaaaaat
    34621 cggccatgat actttgtgcg gtggcgttat cttccggtat cgaacgcttc agtaacgtca
    34681 actggatacg taagtaagaa agtacctgag ccagcgagtc gtgcaattcg cgggcgatgg
    34741 ttgcacgttc ttccatcaac aataactgct gaaaatgctt ctgcgcctga ttaaagtaca
    34801 aaccgcgtcc cagcatcgat gaaacactgt taagcagcgg ttctgatgat gaaacgtgac
    34861 tattttgcca gtgcagttcg ccgtaaaccg tctcttgcat tgtcaccggt aaaatctgca
    34921 tcggcaattc cgggtttggt tgcccttcgc taatccgcca gttttcaccg acatttaact
    34981 ccagatattc agccgcttca ttgtcgcgaa caatctgcaa aatatggcgg aaacaatgca
    35041 catcaatctg gctggtgttc agcgcctggg aacattgata caacacctcc agacgacgct
    35101 tggcctcgtg gagatcgcgg gtcttttctt ctactgacgc ctccagcgaa aggtacaatt
    35161 tatgcagctc gctcgacatc tggttaaagg tttttgcaag cagaccaagc tcattcggca
    35221 gactggtatc cagcggcggc gagtcgaact gcccgtgttc aatacgttga ctggcggtaa
    35281 ccagctgatt cagcggggca accacctgat ggcgtatacg gcgcaaagta aaaaagacca
    35341 gcgtgaaaat accgataccg ccagccaggg aaatcgccac caccagcagc attttgcgtt
    35401 cagcgtagtg ctgtaaagcc agcacgaaca ggtctatctg attaacataa ttattaatat
    35461 tggcctgata ccacggcaaa tcgcccttgc tgagccgatt attcatctcc agccagttgg
    35521 cattcagatg cgcatagcga gtttttactg cttctggcac ataccagacg ttgagattgg
    35581 ttaataccgg tgaatgcagt gcctgctgaa acaactggcg atgtgcattg agttgtggac
    35641 tgccactttg caagtcgtag cccaggcggt aactctgcat acgcagcgat ccggcaatat
    35701 tgatagcctc agcgtcgcgc aaactgctcg ccagagttag cagagcgata cccgtggaaa
    35761 gaatcgacag cagcacaatg taaaaaaagg cccgggccag actggccgag acgggtcgtt
    35821 taacaatcac acgcgtcttc tccacaaaaa atcattaatg ttccagttcg ctgaaacaca
    35881 atttagtcag tgatgggatt atttgatctg ccacagttta tagcgaaata gttacttatt
    35941 ctgggcacat gaacattgcc gattcccctg taagtcgggt aataacaaca ctcatataaa
    36001 gaataaggtt tttacaacca aaaaagaagg tcgttatgaa tcgttttatt atggccaaca
    36061 gtcagcaatg tctgggttgt catgcttgtg aaatcgcctg tgtcatggct cacaatgatg
    36121 agcaacatgt cctgagccaa caccattttc atccccgaat tacggttatc aaacatcaac
    36181 agcaacgtag tgcagtgacc tgtcaccatt gtgaagatgc gccctgcgcc cgtagctgcc
    36241 ctaatggcgc aatcagccac gttgatgaca gcattcaggt caatcagcaa aagtgtattg
    36301 gctgtaaatc ctgcgtggtg gcctgtcctt ttggtacgat gcaaatcgtc ctgacacccg
    36361 tcgcggcagg aaaagtaaaa gccacggcgc ataaatgcga cctttgtgcg gggcgcgaaa
    36421 acggtcctgc ctgtgttgag aattgcccgg cggacgcgct gcaactggtc actgacgtcg
    36481 cactctccgg catggcgaaa tcccgccgct tgcgcaccgc gcgtcaggaa catcaaccgt
    36541 ggcatgccag taccgcggcg caagaaatgc cggtaatgag taaagtcgaa caaatgcagg
    36601 caacgcccgc gcgtggcgag ccggataaac tggcgattga agcgcgcaaa accggttttg
    36661 atgaaattta tctgccattt cgcgccgacc aggcacaacg ggaagcctcg cgctgcctta
    36721 agtgcggcga gcacagcgtt tgtgaatgga cctgcccgct gcataaccat ataccgcagt
    36781 ggattgaact ggtgaaagcc ggaaacatcg acgccgccgt cgagctttct caccagacca
    36841 acaccctgcc ggaaattacc ggacgcgttt gtccgcaaga ccgtttgtgt gaaggtgcct
    36901 gtactattcg cgatgagcac ggcgcggtaa ctatcggcaa cattgaacgc tacatttcag
    36961 atcaggcgtt ggcgaaaggt tggcgtcctg acttaagcca tgtcaccaaa gtggacaagc
    37021 gggtggcgat tatcggtgca ggtccggcag ggctggcctg tgcggatgtt ctgacccgca
    37081 atggcgtggg ggtgacggtg tacgatcgcc atccagaaat cggtggcttg ctcactttcg
    37141 gcattccttc tttcaaactg gataaatccc tgctggcacg ccgtcgggaa atcttcagcg
    37201 cgatggggat tcacttcgaa ctcaattgtg aagtgggtaa agatgtctct ttggattcgc
    37261 ttttggaaca atacgacgcg gtcttcgttg gcgtaggcac ttaccgttcc atgaaagcgg
    37321 gtttacccaa tgaagatgcg ccgggcgttt atgacgcgct gccgttcctc attgccaaca
    37381 ctaaacaggt gatggggctc gaagagctac cggaagagcc gtttatcaat accgccggac
    37441 ttaacgtcgt ggtactgggc ggcggcgaca ccgcgatgga ctgtgtgcgt actgcgctgc
    37501 gccacggcgc gagtaacgtc acctgcgctt atcgtcgtga tgaagctaac atgccaggct
    37561 cgaagaaaga ggtgaggaac gcccgcgaag agggggccaa cttcgaattt aacgtccagc
    37621 cggtggcgct tgagctgaat gaacaaggtc acgtctgcgg gattcgtttc ctgcgcacgc
    37681 gtcttggcga gccggatgcc caggggcgtc ggcgtccagt gccggtggaa ggcagtgaat
    37741 ttgtcatgcc agccgacgcg gtgattatgg cgtttggctt caatccgcac gggatgccgt
    37801 ggctggagtc gcacggtgta acggtagaca aatggggccg catcatcgcg gatgtggaaa
    37861 gccagtaccg ttaccagacc accaatccga aaatcttcgc tggtggtgac gccgtgcgtg
    37921 gtgcggatct ggtggttacc gcaatggcag aaggacgtca tgcggcacag gggattattg
    37981 actggctggg ggtaaaatca gtcaaatctc actgatagcc tgcgcagaca aacccgactt
    38041 cacagcgtaa gataattgtt catttcgcgc tgtggagtcg gtatgacgca acaaatcacc
    38101 ctcattaaag acaaaattct ctccgataac tatttcaccc tgcacaacat tacctacgat
    38161 ctcacgcgca aagatggcga agttatccgc cataaacgtg aagtttacga tcgcggtaat
    38221 ggtgcgacga tcctcctgta caacgcgaag aaaaagagcg tggttctgat tcgtcagttc
    38281 cgtgtcgcta cctgggttaa tggcaatgaa agcgggcagc tgattgaaac ctgcgccggg
    38341 ctgctggata acgacgaacc ggaagtgtgc attcgtaaag aagcgattga agagacgggc
    38401 tatgaagtgg gcgaagtgcg caaattattt gaactgtata tgtcgcctgg cggtgtgact
    38461 gagctaatcc acttttttat cgccgaatac agtgacaatc agcgcgctaa cgccggtggc
    38521 ggtgttgaag atgaagatat tgaagtgctc gagctgccgt tcagccaggc gctggagatg
    38581 atcaaaaccg gcgagatacg tgacggtaag acggtgttat tgcttaacta tttgcaaacg
    38641 tcacatttaa tggactgaaa aataacaata atatttcgtt gtttattatt ggctcaatcc
    38701 gacaaatctg attgagccgc gctacctgcg caacgaagat acgacttgtg ctgtttgttt
    38761 gaacttctgg ggtcgtaccg tccatgcgct atcgcatttt ccttctcttt tttttcgctt
    38821 tgttgccgac gtctttggtg tgggcggcac cagcgcaacg ggcgttttcc gactggcagg
    38881 tcacctgtaa taaccaaaac ttctgcgtgg cgcgcaatac gggcgatcat aatggactgg
    38941 tgatgaccct gagccgcagc gccggggcgc ataccgatgc cgttttacgt attgagcgcg
    39001 gcgggttgaa gtcgccggat gcgtctgaag gagagatagc gccacggctg ctgttagatg
    39061 gtgagccgtt ggcgttaagt ggtgacaagt ggcggatttc accatggtta ttagtaaccg
    39121 atgatacggc aaccatcacc gcgtttttgc agatgattca ggaagggaag gcgatcacct
    39181 tacgcgacgg caatcagacc atttctctga gtggcttaaa agcggcgttg ttgtttattg
    39241 atgctcagca aaagcgcgtt ggcagtgaaa ccgcgtggat caagaaaggg gacgaaccgc
    39301 cgctcagcgt accgcccgcg cctgcgctga aagaggtcgc ggtggttaac ccaacgccga
    39361 caccactctc actcgaagaa cgcaacgatt tgctggatta tggaaactgg cggatgaatg
    39421 gtctgcgctg ctcgcttgat ccgttacgtc gtgaggtgaa tgtcactgcg ctgactgatg
    39481 ataaagcgct gatgatgatt agctgtgagg caggggccta taacaccatt gatttggcat
    39541 ggattgtgtc gcgtaaaaag ccactagcct cgcgcccggt tcggttgcgt ttgccgttca
    39601 acaacggtca ggagacgaat gaactggaac tgatgaacgc aacatttgat gagaaatcgc
    39661 gtgaactggt gaccttagcg aaagggcgcg gattaagcga ttgtggcatt caggcgcgct
    39721 ggcgctttga cggtcagcgt tttcgcctgg tgcgttatgc cgcagaaccc acctgcgata
    39781 actggcatgg gccagacgcc tggcccacgt tgtggatcac ccgttaaaca aaattgcggg
    39841 gacatgtagg ccggataaga cgctgtaggc cggataagac gcgtcagcgt cgcatccggc
    39901 aatcagcatc cggcgatcac catcaggcac ctttcactcc cagcacctta tgcgcttttg
    39961 ccacaatatt ctcggcggta aagccaaaga acgggaacag cttatccgcc ggagcagatt
    40021 ccccgtaacc cgtcatcccg acaattgccc ctttcagacc aacatacttg taccagtaat
    40081 cggcaatacc tgcttccacc gccacgcgag ccgcaacgtt agaaggcaac accgactccc
    40141 gatattcctc atcctgggcg tcgaaaatat cggtcgaggg cagggaaact acgcgtacat
    40201 tgcgaccttc tcctgctaat ttctctgccg cttgcagggt aatttccatc tctgaaccgg
    40261 tggcaatcaa aataatatct ggcttaccgc cgctgtcttt cagcacataa ccgccacgag
    40321 caatctcttt aacctgatcc ggcgtacgtt ccacctgggc cagattctgc ctggagagaa
    40381 tcagtgccgt cggtccgttg tggcgctcaa ccgccagctt ccagcccacc gccgcttcca
    40441 cctgatcgca cggtcgccag gtgctgaaat ttggcgttaa gcgcaggctg gccagttgct
    40501 caacagcctg gtgcgtcgga ccatcttcgc ccaggccaat tgagtcgtgg gtataaacca
    40561 taatctgccg cgctttcatc agtgccgcca tccgcgcggc gttacgggcg tattcaacaa
    40621 acatcaggaa cgtcgcggta tacggcacaa agccgccgtg gtgcgcaatg ccgttggcga
    40681 tagcggtcat gccaaattca cgcaccccgt agtgaatgta gttgcccgcc ggatcttcct
    40741 tcagcgacac agagcctttc cagatggtga ggttgctggg agccagatcc gctgaacctc
    40801 cgagcagctc cggtagcatc ggtccgtaag cgttaagcgt attttgcgaa gccttacggg
    40861 tggcgatttt cgccggattc gcctgcaact cattgatata tttctgagtc gttttctccc
    40921 agtccttcgg taaaccaccg ctcatccgac gggtaaactc ttctgccagt tgcggatgag
    40981 cctttttata ggcggcaaac ttctcattcc agctctgctg cgctttttcg cctttttcgc
    41041 gggcatccca ggcgtgataa atctttttag ggatctcaaa tggcggatgg tgccagccca
    41101 gtttttgccg tgccagcgcc acttcttctt cccccagtgg tgcgccgtgc gcctcttcct
    41161 tacctgcttt attcggcgaa ccaaagccaa tcaccgtacg gcagataatc agcgacggct
    41221 tatctttcac gctttgcgct tcaaggatcg cttccttcac cgcctgcgga tcgtgaccgt
    41281 cgatttcatg gatcacatgc cagtgatagg cttcaaaacg ttttgccgta tcgtcggtaa
    41341 accagccttc tgtttcaccg tcgatggaaa taccgttgtg atcgtaaaaa ccaatcagct
    41401 tgcccagtcc cagcgtgcct gccagcgaac agacttcgtg ggaaatacct tccatcaggc
    41461 agccgtcgcc cataaacaca taggtgaagt gatcgacgat ctcatggtct ggctggttaa
    41521 actgcgccgc cagtgtacgc tctgctatcg ccagcccgac ggcgttcgcc aaaccttgtc
    41581 caagcgggcc ggtggtggtt tcaacgcctg gcgtatagcc aatctccggg tggcctgggg
    41641 ttttcgaatg caactgacgg aagttcttca gttcttccag cggcaggtcg taaccggtca
    41701 gatgtagcaa actgtagagc agcatcgacg cgtgaccgtt ggaaagaata aagcggtcgc
    41761 gatcatacca ggttgggtcg gtaggattat gtttaagaaa atcgttccac agcacttcgg
    41821 caatatcagc catgcccatc ggcgcgccgg gatgaccaga attggctttt tgtaccgcat
    41881 ccatactgag tgcgcgaatc gcattggcaa ggtcttttcg ggacatataa cactccgtgg
    41941 ctggtttata gtttggcggc aagaagatct tccagtttgc gttgatcaac agcgaacaga
    42001 cgaatgcctt cagacagttt ttctaccgcc atcgcatcct gattgtgctc ccaacggaac
    42061 tccgcttcgc tcatgggtgc tgggcgtggg aacgtctgag aaggcgggat taatttacgt
    42121 accactggcg aaactttttc ctgcagctcc ttcagcaaat tcggtgcgat agtcagtcga
    42181 tcgcagccgg ttaaggcgag gatttgttcg gtgcgacgga agctcgcgcc catcacaatg
    42241 gtttcatagt ggtgttgctt atagtagtcg tagatattgc gcaccgattt aacgcccgga
    42301 tcttcttcca ccacatacgg gtccatcggc ttgcgtgcct gataccagtc ataaatacgc
    42361 ccgacaaacg gcgaaatcag aaaaacgccc gcttctgcac aggcccgcgc ctgggcaaaa
    42421 gaaaacagca gcgtcaggtt gcagttaata ccttcttttt ccagctcttc ggccgcgcga
    42481 attccttccc aggtcgaagc cagtttgatc aaaatgcgtg atttctcaac gccttgttgc
    42541 tgatacaagt ccaccagatg gcgcgccttt tcaatactct tttctttatc aaaagagagg
    42601 cgtgcatcaa cttctgttga cacgcgaccg ggtacgattt tgagaatttc agcaccgaaa
    42661 ttgactgcca gtttgtcaca cgctgcgacc acctgttgtt cctgggtctt gccatttttt
    42721 ttaccccagg cgatagcatc gtctattaaa tgctcatatt gtgataatcc ggcagctttg
    42781 agtaacagcg aaggattggt ggtggcatcc tggggatgat aatggcgaat ggactcaata
    42841 tcgccgctgt ctgccacgac agtggtgaac tgtttgatgc cgtctaactc gttcatagga
    42901 aatactcctt gaaaagtaaa gtgttagatg agtgcgttaa ttcacacttc tgagaaattt
    42961 cgctaaacgc atcaaaaaag catagcagac aggcatggta ttgctggatt aagcaggtaa
    43021 catcagtgtt ataggattat taccaaaaca ttatattaat ggtgagagtt tggacttgct
    43081 caaagtctgt agactccggc agggtaataa tgtgcgccac gttgtgggca ggggctgttg
    43141 cccacacact ttatttgtga acgttacgtg aaaggaacaa ccaaatggat gaccagttaa
    43201 aacaaagtgc acttgatttc catgaatttc cagttccagg gaaaatccag gtttctccaa
    43261 ccaagcctct ggcaacgcag cgcgatctgg cgctggccta ctcaccaggc gttgccgcac
    43321 cttgtcttga aatcgaaaaa gacccgttaa aagcctacaa atataccgcg cgcggtaacc
    43381 tggtggcggt gatctctaac ggtacggcgg tgctggggtt aggcaacatt ggcgcgctgg
    43441 ctggcaaacc ggtgatggaa ggcaagggcg ttctgtttaa gaaattcgcc gggattgatg
    43501 tatttgacat tgaagttgat gaactcgacc cggacaaatt tattgaagtt gtcgctgcgc
    43561 tcgaaccgac cttcggcgga atcaacctcg aagacattaa agcgccagaa tgtttctata
    43621 ttgaacagaa actgcgcgag cggatgaata ttccggtatt ccacgacgat cagcacggca
    43681 cggcaatcat cagcactgcc gccatcctca acggcttgcg cgtggtggag aaaaacatct
    43741 ccgacgtgcg gatggtggtt tccggcgcgg gtgccgcagc aatcgcctgt atgaacctgc
    43801 tggtagcgct gggtctgcaa aaacataaca tcgtggtttg cgattcaaaa ggcgttatct
    43861 atcagggccg tgagccaaac atggcggaaa ccaaagccgc atatgcggtg gtggatgacg
    43921 gcaaacgtac cctcgatgat gtgattgaag gcgcggatat tttccttgga tgttccggcc
    43981 cgaaagtgct gacccaggaa atggtgaaga aaatggctcg tgcgccaatg atcctggcgc
    44041 tggcgaaccc ggaaccggaa attctgccgc cgctggcgaa agaagtgcgt ccggatgcca
    44101 tcatttgcac cggtcgttct gactatccaa accaggtcaa caacgtcctt tgcttcccgt
    44161 ttatcttccg tggcgcgctg gatgttggcg caacggctat caatgaagag atgaaactgg
    44221 cggcggtacg cgcgattgcg gaactggccc acgccgaaca gagcgaagtg gtggcttcag
    44281 cgtatggcga tcaggatctg agctttggtc cggaatacat cattccaaaa ccgtttgatc
    44341 cgcgcttgat cgttaagatc gctcctgcgg tcgctaaagc cgcgatggag tcgggcgtgg
    44401 cgacgcgtcc gattgctgat ttcgacgtct acatcgacaa gctgactgag ttcgtttaca
    44461 aaaccaacct gtttatgaag ccgattttct cccaggctcg caaagcgccg aagcgcgttg
    44521 ttctgccaga aggggaagag gcgcgcgttc tgcatgccac tcaggaactg gtaacgctgg
    44581 gactggcgaa accgatcctt atcggtcgtc cgaacgtgat cgaaatgcgc attcagaaac
    44641 tgggcttgca gatcaaagcg ggcgttgatt ttgagatcgt caataacgaa tccgatccgc
    44701 gctttaaaga gtactggacc gaatacttcc agatcatgaa gcgtcgcggt gtcactcagg
    44761 aacaggcgca gcgggcgctg atcagtaacc cgacagtgat cggcgcgatc atggttcagc
    44821 gtggggaagc cgatgcaatg atctgcggta cggtgggcga ttatcatgaa cattttagcg
    44881 tggtgaaaaa tgtctttggt tatcgcgatg gcgttcacac cgcaggtgca atgaacgcgc
    44941 tgctgctgct gagtggtaac acctttattg ccgataccta cgtcaatgac gaaccagacg
    45001 cagaagagct ggcggagatc accttgatgg cggcagaaac tgtccgtcgt tttggtattg
    45061 agccgcgcgt tgctctgttg tcgcactcca actttggttc ttctgactgc ccgtcgtcga
    45121 gcaaaatgcg tcaggcgctg gaactggtca gggatcgtgc gccagatctg atgattgatg
    45181 gtgaaatgca cggcgatgca gcgctggtgg aagcgattcg caacgaccgt atgccggaca
    45241 gccctttgaa aggttctgcc aatattctgg tgatgccgaa catggaagct gcccgcatta
    45301 gttacaactt actgcgcgtt tccagctcgg aaggtgtgac tgtcggtccg gtgctgatgg
    45361 gcgtggcgaa accggttcac gtgttaacgc cgatcgcatc ggtgcgtcgt atcgtcaaca
    45421 tggtggcgct ggccgtggtt gaagcgcaaa cccaaccgct gtaattcttt tttaactctc
    45481 acgcttaccc tgaatattca gggtaagcgg tttggctgca atatattagt aaagcttatt
    45541 actgagt
//
LOCUS       DAPHDX010000020        45210 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPHDX010000020 DAPHDX010000000
VERSION     DAPHDX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.020"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            158..493
                     /gene="eutS"
                     /locus_tag="RH058_001076"
     CDS             158..493
                     /gene="eutS"
                     /locus_tag="RH058_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV3136696.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            506..985
                     /gene="eutP"
                     /locus_tag="RH058_001077"
     CDS             506..985
                     /gene="eutP"
                     /locus_tag="RH058_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV3136697.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            960..1661
                     /gene="eutQ"
                     /locus_tag="RH058_001078"
     CDS             960..1661
                     /gene="eutQ"
                     /locus_tag="RH058_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV3136698.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1658..2461
                     /gene="eutT"
                     /locus_tag="RH058_001079"
     CDS             1658..2461
                     /gene="eutT"
                     /locus_tag="RH058_001079"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV3136699.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2458..3474
                     /gene="pta"
                     /locus_tag="RH058_001080"
     CDS             2458..3474
                     /gene="pta"
                     /locus_tag="RH058_001080"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV3136700.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3513..3806
                     /gene="eutM"
                     /locus_tag="RH058_001081"
     CDS             3513..3806
                     /gene="eutM"
                     /locus_tag="RH058_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV3136701.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3913..4200
                     /gene="eutN"
                     /locus_tag="RH058_001082"
     CDS             3913..4200
                     /gene="eutN"
                     /locus_tag="RH058_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV3136702.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4212..5615
                     /locus_tag="RH058_001083"
     CDS             4212..5615
                     /locus_tag="RH058_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV3136703.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5626..6462
                     /gene="eutJ"
                     /locus_tag="RH058_001084"
     CDS             5626..6462
                     /gene="eutJ"
                     /locus_tag="RH058_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV3136704.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6452..7639
                     /gene="eutG"
                     /locus_tag="RH058_001085"
     CDS             6452..7639
                     /gene="eutG"
                     /locus_tag="RH058_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV3136705.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7756..8982
                     /gene="eutH"
                     /locus_tag="RH058_001086"
     CDS             7756..8982
                     /gene="eutH"
                     /locus_tag="RH058_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV3136706.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8979..10382
                     /gene="eutA"
                     /locus_tag="RH058_001087"
     CDS             8979..10382
                     /gene="eutA"
                     /locus_tag="RH058_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV3136707.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATTLHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10394..11755
                     /gene="eutB"
                     /locus_tag="RH058_001088"
     CDS             10394..11755
                     /gene="eutB"
                     /locus_tag="RH058_001088"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV3136708.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11776..12663
                     /gene="eutC"
                     /locus_tag="RH058_001089"
     CDS             11776..12663
                     /gene="eutC"
                     /locus_tag="RH058_001089"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV3136709.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12673..13332
                     /gene="eutL"
                     /locus_tag="RH058_001090"
     CDS             12673..13332
                     /gene="eutL"
                     /locus_tag="RH058_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV3136710.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13345..13845
                     /gene="eutK"
                     /locus_tag="RH058_001091"
     CDS             13345..13845
                     /gene="eutK"
                     /locus_tag="RH058_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV3136711.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13891..14943
                     /gene="eutR"
                     /locus_tag="RH058_001092"
     CDS             13891..14943
                     /gene="eutR"
                     /locus_tag="RH058_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV3136712.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14949..15848)
                     /gene="hemF"
                     /locus_tag="RH058_001093"
     CDS             complement(14949..15848)
                     /gene="hemF"
                     /locus_tag="RH058_001093"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV3136713.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15852..16721)
                     /gene="amiA"
                     /locus_tag="RH058_001094"
     CDS             complement(15852..16721)
                     /gene="amiA"
                     /locus_tag="RH058_001094"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV3136714.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16935..17360
                     /locus_tag="RH058_001095"
     CDS             16935..17360
                     /locus_tag="RH058_001095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV3136715.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17347..17796
                     /locus_tag="RH058_001096"
     CDS             17347..17796
                     /locus_tag="RH058_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV3136716.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALVWLLTNRRLRACFNEVKE"
     gene            17857..18432
                     /locus_tag="RH058_001097"
     CDS             17857..18432
                     /locus_tag="RH058_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV3136717.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18528..19427
                     /gene="yfeX"
                     /locus_tag="RH058_001098"
     CDS             18528..19427
                     /gene="yfeX"
                     /locus_tag="RH058_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV3136718.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19485..20789)
                     /gene="pbp4b"
                     /locus_tag="RH058_001099"
     CDS             complement(19485..20789)
                     /gene="pbp4b"
                     /locus_tag="RH058_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV3136719.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20794..22218)
                     /gene="murP"
                     /locus_tag="RH058_001100"
     CDS             complement(20794..22218)
                     /gene="murP"
                     /locus_tag="RH058_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV3136720.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22222..23118)
                     /gene="murQ"
                     /locus_tag="RH058_001101"
     CDS             complement(22222..23118)
                     /gene="murQ"
                     /locus_tag="RH058_001101"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV3136721.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23282..24139
                     /gene="murR"
                     /locus_tag="RH058_001102"
     CDS             23282..24139
                     /gene="murR"
                     /locus_tag="RH058_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV3136722.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCSLFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24268..25059
                     /gene="ucpA"
                     /locus_tag="RH058_001103"
     CDS             24268..25059
                     /gene="ucpA"
                     /locus_tag="RH058_001103"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV3136723.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25230..26246
                     /gene="cysP"
                     /locus_tag="RH058_001104"
     CDS             25230..26246
                     /gene="cysP"
                     /locus_tag="RH058_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV3136724.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26246..27079
                     /gene="cysT"
                     /locus_tag="RH058_001105"
     CDS             26246..27079
                     /gene="cysT"
                     /locus_tag="RH058_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV3136725.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27079..27954
                     /gene="cysW"
                     /locus_tag="RH058_001106"
     CDS             27079..27954
                     /gene="cysW"
                     /locus_tag="RH058_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV3136726.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27944..29041
                     /gene="cysA"
                     /locus_tag="RH058_001107"
     CDS             27944..29041
                     /gene="cysA"
                     /locus_tag="RH058_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV3136727.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29175..30086
                     /gene="cysM"
                     /locus_tag="RH058_001108"
     CDS             29175..30086
                     /gene="cysM"
                     /locus_tag="RH058_001108"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV3136728.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30089..30457)
                     /locus_tag="RH058_001109"
     CDS             complement(30089..30457)
                     /locus_tag="RH058_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV3136729.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30562..31413
                     /gene="pdxK"
                     /locus_tag="RH058_001110"
                     /gene_synonym="thiJ"
     CDS             30562..31413
                     /gene="pdxK"
                     /locus_tag="RH058_001110"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV3136730.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31455..31964)
                     /gene="crr"
                     /locus_tag="RH058_001111"
     CDS             complement(31455..31964)
                     /gene="crr"
                     /locus_tag="RH058_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV3136731.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32005..33732)
                     /gene="ptsI"
                     /locus_tag="RH058_001112"
     CDS             complement(32005..33732)
                     /gene="ptsI"
                     /locus_tag="RH058_001112"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV3136732.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33777..34034)
                     /gene="ptsH"
                     /locus_tag="RH058_001113"
     CDS             complement(33777..34034)
                     /gene="ptsH"
                     /locus_tag="RH058_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV3136733.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34418..35389)
                     /gene="cysK"
                     /locus_tag="RH058_001114"
     CDS             complement(34418..35389)
                     /gene="cysK"
                     /locus_tag="RH058_001114"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV3136734.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35574..36335)
                     /gene="cysZ"
                     /locus_tag="RH058_001115"
     CDS             complement(35574..36335)
                     /gene="cysZ"
                     /locus_tag="RH058_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV3136735.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGSFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36565..37551
                     /gene="zipA"
                     /locus_tag="RH058_001116"
     CDS             36565..37551
                     /gene="zipA"
                     /locus_tag="RH058_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV3136736.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37622..39637
                     /gene="ligA"
                     /locus_tag="RH058_001117"
     CDS             37622..39637
                     /gene="ligA"
                     /locus_tag="RH058_001117"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV3136737.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39639..39857
                     /locus_tag="RH058_001118"
     CDS             39639..39857
                     /locus_tag="RH058_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV3136738.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39854..40852)
                     /locus_tag="RH058_001119"
     CDS             complement(39854..40852)
                     /locus_tag="RH058_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV3136739.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40942..41868
                     /locus_tag="RH058_001120"
     CDS             40942..41868
                     /locus_tag="RH058_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136740.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41907..42671)
                     /locus_tag="RH058_001121"
     CDS             complement(41907..42671)
                     /locus_tag="RH058_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136741.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            42920..43753
                     /gene="xapA"
                     /locus_tag="RH058_001122"
     CDS             42920..43753
                     /gene="xapA"
                     /locus_tag="RH058_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDV3136742.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            43813..45069
                     /gene="xapB"
                     /locus_tag="RH058_001123"
     CDS             43813..45069
                     /gene="xapB"
                     /locus_tag="RH058_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDV3136743.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTVGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            45122..>45210
                     /locus_tag="RH058_001124"
     CDS             45122..>45210
                     /locus_tag="RH058_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HDV3136744.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNV"
BASE COUNT        10541 a        11483 c        12626 g        10560 t
ORIGIN      
        1 atattcatgc gttgcatatg aaagtttatg caccacagcg aatatctctc cattcttagt
       61 gatctacctc accttttaaa cgcgcttgcc gaattttgtt atttactctg acgaaaaatt
      121 gtcacgatac acgaaagttt ttcacaggcg gcgactcatg gataaagaac gcatcattca
      181 ggaatttgtg cccggcaaac aggtcacact ggcgcatctc atcgcgcatc ccggtgagga
      241 actggcgaag aaaatcggcg ttccggatgc gggcgcaatt ggcattatga cgctaactcc
      301 cggcgaaacg gcgatgatcg ccggtgattt agcgctgaaa gccgctgatg tgcatatcgg
      361 ttttctcgac cgcttcagcg gtgcattggt gatttatggc tcggtgggcg cagtagaaga
      421 agcgttatca caaaccgtca gcggtttagg tcgcttatta aattatacgc tttgtgaaat
      481 gaccaaaagt taatacgagg cattcatgaa acgaattgcg tttgtgggta gcgttggggc
      541 aggaaaaaca acgttattta atgcgttgca ggggaattat accctcgcca gaaaaacaca
      601 ggccgtggaa tttaatgata agggcgatat tgatacgccg ggtgaatatt ttagccatcc
      661 ccgctggtat cacgccttaa ttaccacgct acaggatgta gacatgctga tctatgtcca
      721 tggcgcaaat gacccggaaa gtcggttgcc tgccgggttg ctggatattg gcgtcagcaa
      781 acggcaaatc gccgtcatca gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg
      841 aaagttattg cttgagacgg gtttcgaaga gccgattttc gagctaaaca gccacgaccc
      901 gcaaagcgtg cagcaactgg tggattatct ggcatctctc accaaacagg aggaagcagg
      961 tgaaaaaact catcacagcg aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa
     1021 tgtccgtggt tctgcgcgcc agcattatta ccccggaagc ccgtgaagtg gcggatctac
     1081 tgggttttac catcaccgaa tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca
     1141 gcgtgccagc cgacaaaacc gaaagccagc gcatccgcga aaccatcatt gcccagctgc
     1201 cggaaggcca gtttaccgaa agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa
     1261 aacagtcgct ggagcagggc gcaatgcagc cgagcttcaa atcggtgacc ggcaaaggcg
     1321 gcatcaaagt gattgatggc agcagcgtca agtttggtcg tttcgacggc gcagagccgc
     1381 attgcgtggg cttgactgat cttgtgaccg gagacgacgg cagcagcatg gccgctggtt
     1441 ttatgcagtg ggagaacgca ttcttcccgt ggaccctgaa ctacgacgaa atcgacatgg
     1501 tgctggaagg ggagctgcat gttcgccacg aaggcgaaac catgatcgcc aaagcgggtg
     1561 acgtgatgtt tatcccgaaa ggctccagta tcgaatttgg tacgacatcc agcgtgaaat
     1621 tcctgtatgt cgcctggccg gctaactggc aatccctatg aaagatttca tcaccgaagc
     1681 atggctaaga gcgaaccata cgctcagcga aggcgcagag atccatctcc ctgcggacag
     1741 tcgcctgacg ccctctgccc gggagttact ggaaagccgc catctgcgca tcaagtttat
     1801 tgacgagcag ggccgcctgt ttgttgacga tgaacagcag cagccgcagc ccgttcatgg
     1861 gctgaccagt agcgatgaac atccgcaggc gtgctgcgaa ctgtgccgcc agccggtggc
     1921 aaaaaagccg gatacgctga ctcacctgtc ggcggaaaaa atggtcgcca aaagcgatcc
     1981 gcgtctgggt tttcgcgcgg ttctcgacag caccattgcg ctggcagtgt ggctgcaaat
     2041 tgaactggcg gaaccgtggc agccgtggct ggcggatatc cgttcgcgtc ttggcaacat
     2101 tatgcgcgcc gatgcgctgg gtgaaccgct gggctgccag gcgattgtcg ggctttctga
     2161 cgaagatctg catcgacttt ctcaccagcc gctgcgctat ctcgaccacg atcatctggt
     2221 gccggaagcc agccacgggc gcgatgcggc gttgctcaat ctgctacgta ccaaagtgcg
     2281 ggaaaccgaa accgtagcgg cgcaggtgtt tatcacccgc agctttgaag tattacgccc
     2341 ggacattttg caggcgctga accgcctttc cagcacggtc tacgtgatga tgatcctgag
     2401 cgtaacgaag cagccgctaa cggtgaagca gatccaacaa cgactggggg aaacgcaatg
     2461 attattgaac gttgtcgtga actggcgttg cgagcgcccg ccagagtggt ttttccggat
     2521 gcgttagacc aacgtgtgct gaaagccgcg caatatttac atcaacaagg tctggcaacg
     2581 cccattctgg tcgccaatcc gtttgaactt cgtcagtttg cgctcagtca cggcgtggcg
     2641 atggacgggc tacaggtgat tgatccgcat ggcaacctcg caatgcggga agaatttgct
     2701 catcgctggc tggcccgcgc gggcgaaaaa acgccgccgg atgcgctgga aaaacttacc
     2761 gaccctctga tgttcgccgc agcaatggtc agcgccggta aagcggatgt ctgtatcgcg
     2821 ggcaacctct cttccacggc aaatgtgctg cgtgccggat tacgcattat cggcttgcag
     2881 ccaggctgta aaacgctctc ttccattttc ctgatgttgc cacagtacag cggcccggcg
     2941 ttgggctttg ccgattgcag cgtggtgcca cagccgacgg cggcgcagct ggcggatatc
     3001 gcgcttgcca gtgccgaaac ctggcgcgcc atcaccggag aagagccgcg cgtggcgatg
     3061 ctgtcgtttt ccagcaatgg tagcgcccgt cacccctgtg ttgctaacgt ccagcaggcg
     3121 acagaaatcg tccgtgagcg cgcaccaaag ctggtggtgg atggcgagtt acagtttgac
     3181 gccgccttcg tgccggaagt ggcggcgcaa aaagcgcctg ccagcccgct acagggcaag
     3241 gccaatgtga tggtttttcc gtcgctggaa gccggaaata ttggttacaa aatcgcacaa
     3301 cgactcggcg gatatcgtgc cgtcgggcca ctgatacaag gacttgccgc gccgatgcac
     3361 gatctctctc gtggttgtag cgtgcaggaa attatcgagc tggcgctggt ggcagctgtg
     3421 ccgcgtcaga cagaagtgaa ccgcgaaagc agtttacaaa cactggttga atgaacggtc
     3481 ccgttctgga ccctgataaa gaggaaagca caatggaagc attaggaatg atcgaaaccc
     3541 ggggcctggt tgcactgatt gaggcttctg acgcgatggt gaaagctgcg cgcgtcaagc
     3601 tggttggtgt taagcagatt ggcggcggcc tgtgtactgc catggttcgt ggtgatgttg
     3661 cagcctgtaa agccgcgacc gatgcaggtg ccgcagcggc acagcgtatc ggcgagctgg
     3721 tatctgtaca cgttatcccg cgtccgcacg gcgacctgga agaagtcttc ccgatcggcc
     3781 tgaaaggcga tagcagcaac ctgtaatcac cctgtgtgac gtcgattcgg gcgtcacgct
     3841 ctctcctttt tcattttacc ttctgcggag ggtaggggga gcttttacct gaaatacgcc
     3901 acggaggcgg gtatgaaact ggcagtcgtc actggacaaa ttgtttgtac cgtacgccat
     3961 cacggactgg cgcatgacaa attgctgatg gtggaaatga ttgatccaca aggtaatcct
     4021 gacgggcaat gcgccgtcgc catcgacaat attggcgcgg gaaccgggga gtgggtgtta
     4081 ctggtgagtg gcagttccgc ccgccaggcg cataaaagcg aaacgtcacc ggtcgatctg
     4141 tgcgtgattg gcattgtcga tgaggtggtg tctggcggtc aggtaatttt ccacaaataa
     4201 ggcagaacat catgaatcaa caggatattg aacaggtggt gaaagcggta ctgctgaaaa
     4261 tgcaaagcag tgacacgccg cccgccgccg ttcatgagat gggcgttttc gcgtccctgg
     4321 atgacgccgt tgcggcagcc aaagtcgccc agcaagggtt aaaaagcgtg gcaatgcgcc
     4381 agttagccat tgctgccatt cgtgaagcag gcgaaaaaca cgccagagat ttagcggaac
     4441 ttgccgtcag tgaaaccggc atggggcgcg ttgaagataa atttgcaaaa aacgtcgctc
     4501 aggcgcgcgg cacaccaggc gttgagtgcc tctctccgca agtgctgact ggcgacaacg
     4561 gcctgaccct aattgaaaac gcaccctggg gcgtggtggc ttcggtgacg ccttccacta
     4621 acccggcggc aaccgtaatt aacaacgcca tcagcctgat tgccgcgggc aacagcgtca
     4681 tttttgcccc gcatccggcg gcgaaaaaag tctcccagcg ggcgattacg ctgctcaacc
     4741 aggcgattgt tgccgcaggt gggccggaaa acttactggt tactgtggca aatccggata
     4801 tcgaaaccgc gcaacgcttg ttcaagtttc cgggtatcgg cctgctggtg gtaaccggcg
     4861 gcgaagcggt agtagaagcg gcgcgtaaac acaccaataa acgtctgatt gccgcaggcg
     4921 ctggcaaccc gccggtagtg gtggatgaaa ccgccgacct cgcccgtgcc gctcagtcca
     4981 tcgtcaaagg cgcttctttc gataacaaca tcatttgtgc cgacgaaaag gtactgattg
     5041 ttgttgatag cgtagccgat gaactgatgc gtctgatgga aggccagcac gcggtgaaac
     5101 tgaccgcaga acaggcgcag cagctgcaac cggtgttgct gaaaaatatc gacgagcgcg
     5161 gaaaaggcac cgtcagccgt gactgggttg gtcgcgatgc ggcgaaaatc gcggcggcaa
     5221 tcggcctgaa cgtgccgcaa gaaacgcgtt tgctgtttgt ggaaaccact gcagaacatc
     5281 cgtttgccgt gaccgaactg atgatgccgg tgctgcccgt cgtgcgcgtc gccaacgtgg
     5341 cggatgccat tgcgctggcg gtgaaactgg agggcggttg ccaccacacg gcggcaatgc
     5401 actcgcgcaa tatcgaaaac atgaaccaga tggcgaacgc cattgatacc agcattttcg
     5461 ttaagaacgg accgtgcatt gccgggctgg ggctgggcgg ggaaggctgg accaccatga
     5521 ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg gacgtttgtc cgtctgcgtc
     5581 gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aaccgatggc gcacgacgaa
     5641 caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac gcctgccgcg
     5701 acggaatctc cgctgtggct gggggtggat ctgggcacct gcgatgtggt gtcgatggtt
     5761 gttgaccgcg acggtcagcc ggtggcggta tgcctcgact gggccgatgt ggtccgcgac
     5821 ggcatcgtct gggatttctt cggcgctgtc accattgttc gtcgccatct cgacacgctc
     5881 gagcagcaat tcggtcgccg ttttagccat gcggcgactt catttcctcc aggtacggac
     5941 ccgcgtattt ccatcaacgt gctggagtct gctggactgg aagtgagcca tgtgctggat
     6001 gagccaaccg cagttgcgga tctgctgcaa ctggacaacg ccggtgtagt ggatatcggc
     6061 ggcggcacta ccggcattgc catcgtgaaa aagggcaaag tgacgtactc ggcggatgaa
     6121 gccaccggcg gacatcacat ctctctgact ctcgccggaa atcgccgtat ttcgctggaa
     6181 gaggcggagc agtacaagcg cggtcatggt gacgagattt ggcccgcggt gaaaccggtg
     6241 tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat tactgattta
     6301 tggctggcgg gcggctcctg tatgcaaccg ggcgtggcgg aactgtttcg caaacaattc
     6361 ccggcgttac aagtgcattt accgcagcac agtttgttta tgaccccgct ggcgatcgcc
     6421 agtagtgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga ccgcgctctt
     6481 tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc caccggtgac
     6541 gctttgcggt ccgggctcgg tgagcagttg cggacagcaa gcgcaaacgc gtgggctgaa
     6601 acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg ccgggctgac
     6661 gcgcagcctg gctgttaaag gcatcgccat gacgctctgg ccatgtccgg tgggcgaacc
     6721 gtgcattacc gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggtt gtgacggggt
     6781 gatcgcattt ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt tgctggtgac
     6841 gaacccggat agcacgctgg cagagatgtc agaaaccagc gttctgcaac cgcgcttgcc
     6901 gctgattgcc attccaacta ccgccggaac cggctctgaa accaccaatg taacggtgat
     6961 tatcgacgcg gtgagcgggc gcaagcaggt gttagcccat gcctcgctga tgccggatgt
     7021 ggcgatcctc gacgccgcat tgaccgaagg tgtgccgtcg catgtcacgg cgatgaccgg
     7081 cattgatgcg ttaacccatg ccattgaagc atacagcgcc ctgaacgcta caccgtttac
     7141 cgacagtctg gcgattggtg ccattgcgat gattggcaaa tcgctgccga aagcggtggg
     7201 ctacggtcac gaccttgccg cgcgcgagag catgttgctg gcttcatgta tggcgggaat
     7261 ggcgttttcc agtgcgggtc ttgggttgtg ccacgcgatg gcgcatcagc cgggcgcggc
     7321 gctgcatatt ccgcacggtc tggcgaacgc catgttgctg ccaacagtga tggaatttaa
     7381 ccggatggtt tgtcgtgaac gctttagtca gattggtcgg gcactgcgaa ctaaaaaatc
     7441 cgacgatcat gacgctatta acgcggtaag tgagctgatt gcggaagttg ggattggtaa
     7501 acgactgggc gatgttggcg cgacatctgc gcattacggt gcatgggcgc aggccgcgca
     7561 ggaagatatt tgtctgcgca gcaacccgcg taccgccagc ctggagcaga ttgtcggctt
     7621 gtacgcagcg gcgcaataaa tgccggatgc gacgcttgcc gcgtctgatc cggccttcgg
     7681 gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccaataaa taacaactaa
     7741 cagggagtaa aggcgatggg aattaacgaa atcatcatgt acatcatgat gttctttatg
     7801 ctgatagctg ccgtagacag gatcctgtcg cagttcggcg gttctgctcg tttcctcggt
     7861 aagttcggta aaagtatcga aggatctggc ggtcagttcg aagaaggctt tatggcaatg
     7921 ggcgctctgg gtctggcgat ggtcggtatg accgcgctgg caccggtact ggctcacgta
     7981 ctcgggccgg taattatccc ggtttacgaa atgctcggcg caaacccgtc aatgttcgcc
     8041 ggaacgctgc tggcgtgcga tatgggcggc ttcttcctcg ccaaagagct ggcgggcggc
     8101 gacgtagcag cgtggctata ctctgggtta attctcgggt cgatgatggg gccaacgatt
     8161 gtgttttcca ttccggtggc gctcggcatt atcgaacctt ctgatcgtcg ttatctggcg
     8221 ctcggcgtgc tggcgggcat tgtgaccatt ccgattggct gtattgctgg tggtctggtt
     8281 gctatgtact ccggtgtgca gatcaacggt cagccggtgg aattcacttt cgccctgatc
     8341 ctgatgaaca tgatcccggt gatcattgtt gcgattctgg tggcgctggg gctgaaattc
     8401 atcccggaaa aaatgatcaa cggcttccag atcttcgcca aattcctcgt tgcattgatc
     8461 accctcggtc ttgccgctgc ggtagtgaaa ttcctgcttg gctgggaact gatccccggt
     8521 ctggatccta tctttatggc ccctggcgat aaacccggtg aagtgatgcg cgccattgaa
     8581 gttatcggtt ctatctcctg cgtgctgtta ggggcgtatc caatggtgct gctgctgact
     8641 cgctggtttg aaaaaccgct gatgagcgtc ggtaaagtac tgaatatgaa caacatcgcg
     8701 gcagccggca tggtggcaac gcttgccaac aacatcccga tgttcggcat gatgaagcag
     8761 atggataccc gcggcaaagt catcaactgc gccttcgccg tttccgctgc tttcgccctg
     8821 ggcgaccact taggcttcgc cgctgccaac atgaacgcca tgatcttccc gatgattgtc
     8881 ggcaagttga tcggcggcgt aacggcgatt ggcgtggcga tgatgctggt gccaaaagaa
     8941 gacgcgaccg cgactaaaac cgaagcggag gcacaatcgt gaacactcgc cagctattga
     9001 gcgtcggtat cgatatcggc accaccacca cccaggtgat tttctcccac ctggagctgg
     9061 ttaaccgtgc ggcggtgtcg caggtgccgc gctacgaatt cattaaacgc gaaattagct
     9121 ggcaaagtcc ggtgttcttt acccctgtcg ataaacaggg cggtttaaaa gaagcggaac
     9181 tgaaaacctt aatactcgag caatatcagg ctgcgggtat tgcgccggaa agcgttgatt
     9241 ctggtgccat catcatcacc ggtgaaagcg cgaaaacccg caatgctcgc ccggcggtga
     9301 tggcgctctc tcagtcgctg ggcgattttg tcgttgccag cgccgggccg cacctcgaat
     9361 ccgtgatcgc cggtcacggt gccggggcgc aaaccctttc tgaacaacgg ctgtgtcggg
     9421 tactgaatat cgacatcggc ggtggcaccg cgaactacgc cctgttcgat gccggaaaaa
     9481 tcagcggcac tgcctgcctc aacgtcggcg gtcgcctgct ggaaaccgac agccaggggc
     9541 gcgtggttta cgctcataaa ccggggcaga tgattgtgga tgagtgcttc ggtgcaggca
     9601 ccgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt tacccggcgg atggcagagc
     9661 tgattgtcga agtgattgac ggaacgcttt cgccgctcgc gcaggcatta atgcaaaccg
     9721 gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct ttctggcggc gtgggcgaat
     9781 gttatcgcca ccagcccgcc gatccgttct gttttgccga tattggcccg ctgctggcaa
     9841 cgacgctgca tgaccatccg cgcctgcgtg agatgaatgt gcagtttccg gcgcaaaccg
     9901 tacgcgccac ggtgattggc gcgggcgcac ataccctttc gctctctggc agcacaatct
     9961 ggctggaggg cgtacaactg ccgctgcgca atttgccggt ggcgatccca attgatgaaa
    10021 cggatctggt gagtgcctgg caacaggcgc tgattcagct ggatcttgat cccaaaactg
    10081 acgcgtacgt gctggcgctt cccgcctcgc tgcctgtgcg ttacgccgcg gtactgacgg
    10141 tcatcaacgc gctggtcgat ttcgtcgcgc gttttccgaa tccgcatccc ctgctggtgg
    10201 tggccgggca ggactttggt aaagctctgg gcatgttgtt gcgcccacag ctacaacaac
    10261 tcccgttggc agtcattgac gaagtgattg tccgcgcggg ggactatatc gacattggta
    10321 cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa atcactcgca tttccttcct
    10381 gagggaacga cttatgaaac taaagaccac attgttcggc aatgtatatc agtttaagga
    10441 tgtaaaagag gtgctggcta aagccaacga actgcgttcg ggggatgtgc tggcaggcgt
    10501 tgcagcggca agttcacagg agcgcgtggc ggcaaagcag gtgttgtcgg aaatgaccgt
    10561 agcggacatc cgcaataatc cggtgattgc ctatgaagat gactgcgtga cgcggctgat
    10621 tcaggacgat gttaacgaaa cggcctacaa ccagattaaa aactggagca tcagcgaact
    10681 gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac attgccttta cccgcaaagg
    10741 gctgacctcg gaagtggtcg cggcggtagc gaagatttgc tccaacgcgg acctgatcta
    10801 cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc accatcggta ttccgggcac
    10861 ctttagcgcc cgtttgcagc caaatgacac ccgtgacgac gtgcaaagta tcgccgcgca
    10921 aatctacgaa gggctttcct tcggagtggg cgatgcggtg atcggcgtta acccggtgac
    10981 agacgacgtg gaaaacttaa gccgcgtgct ggataccatt tatggcgtga tcgacaaatt
    11041 caacatccca actcagggct gcgtactggc gcacgtcacc acccagatcg aagcgattcg
    11101 tcgcggcgca ccaggcgggc tgattttcca gagtatctgt ggcagcgaaa aagggctgaa
    11161 agagtttggc gtggaactgg cgatgctcga cgaagcgcgc gcagtgggtg cagagttcaa
    11221 ccgtatcgcc ggggaaaact gcctctactt cgaaaccgga caaggctctg cgctatccgc
    11281 tggcgctaac ttcggcgctg accaggtgac gatggaagca cgtaactatg ggctggcgcg
    11341 tcattacgat ccgtttatcg tcaacaccgt ggtcggcttt attgggccgg agtatctcta
    11401 caacgaccgc cagattatcc gtgctggctt agaagatcac tttatgggca agctgagcgg
    11461 catctctatg ggctgtgact gctgttacac caaccacgct gacgctgacc agaacctcaa
    11521 cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac tacatcatgg ggatgccgct
    11581 gggtgatgac atcatgctca actaccagac caccgcattc cacgataccg ccactgtgcg
    11641 tcagttactc aacctgcgtc cgtcaccgga gtttgaacgc tggctggaaa gcatgggcat
    11701 tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg tcactgttct tctgatgacg
    11761 cggagataac acatcatgga tcaaaaacag attgaagaaa ttgtacgcag cgtgatggcg
    11821 tcaatgggac aaacggcccc cgcgccgtca gaagcaaagt gcgccaccac caactgtgcg
    11881 gcaccggtga cctcggaaag ctgtgcgctg gatttaggtt ccgctgaagc aaaagcgtgg
    11941 attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgccg cagcaccgtg
    12001 gcccgcgttt gtaccggtcg tgccggtccg cgtccacgta cccaggcgct gctgcgtttc
    12061 ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg
    12121 aaagcgcagg gcttgctgga agtacgctct gaaattagcg acaaaaatct ctacctgact
    12181 cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt
    12241 gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc
    12301 gtgaactacg aagagatcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa
    12361 gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatcggtgag
    12421 atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt agggcagtca
    12481 gaaagcctct cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat
    12541 cgcacctgta tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc
    12601 attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga
    12661 taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg
    12721 attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc
    12781 ctcgggctga tttctgctga ttccgatgac gtcacctaca ttgccgctga tgaagcgacc
    12841 aagcaggcga tggtggaagt ggtatatggt cgctcgctgt atgcgggcgc tgcacacggc
    12901 ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg
    12961 cgtgccggtc tggatgcgat ggttgcacat attgaaaatg gtgcagcatt ccagtgggcc
    13021 aatgacgcag aaaacaccgc cttcctggcg catgtggttt cgcgtaccgg ttcttatctc
    13081 tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg
    13141 gaagcgacct atggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat
    13201 gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg
    13261 tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc
    13321 cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc
    13381 atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc
    13441 agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg
    13501 tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc
    13561 cgcaaggaga tcggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc
    13621 cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac
    13681 gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc
    13741 cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg
    13801 acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc
    13861 gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac
    13921 catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat
    13981 aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg
    14041 cagcagattt acgatcagct gcatccgggc aagtttcatg gtgaatttac ggaaattcta
    14101 ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg
    14161 gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc
    14221 ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa
    14281 ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc
    14341 cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg
    14401 gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg
    14461 gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg
    14521 gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg
    14581 gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg
    14641 gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc
    14701 cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa
    14761 cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg
    14821 gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgc cacggattac
    14881 cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg
    14941 tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg
    15001 ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc
    15061 gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg
    15121 aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc
    15181 atcgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc
    15241 tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg
    15301 ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca ccactttttg
    15361 taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag
    15421 tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac
    15481 cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg
    15541 ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc
    15601 ccggcaagtt ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc gtggacgtga
    15661 gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg
    15721 ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc
    15781 agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg
    15841 ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa
    15901 ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac
    15961 agccgctctt cttccggatt ggtgataaac gaggtttcca ccagcaccga aggaaccgac
    16021 ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc
    16081 ggcttaatct ttttcagaat atgcgagccg agcgtcaggc tgtttttaat agtatcggtt
    16141 tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg
    16201 gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc
    16261 ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg
    16321 gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa
    16381 agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa
    16441 atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt
    16501 ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg
    16561 ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg
    16621 gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg
    16681 cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa
    16741 taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat
    16801 tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt
    16861 ttcgtcagat attggctaat tcgaccgttc acgccataat cagcctttta acccgcaaaa
    16921 aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca
    16981 ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc
    17041 gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg
    17101 gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc
    17161 cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga
    17221 ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg
    17281 gaatgtatga acgcctggga tatgaacacg ccgacgtgct gagtctgggt aagcgtttga
    17341 ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt
    17401 ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc
    17461 attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta tcccgatcac
    17521 gataatttct ggctggggct gattccaggc attccggcgg tgctggcgtt tttgttgagc
    17581 ggtcggcggg ctacatttcc tcgcacctgg cgtgtactct attttctgtt gctgttggcg
    17641 caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt
    17701 atcggtctgg cgctggtagt ggcggatatt gtggcgttag tctggctgct gaccaatcga
    17761 cgtttacgcg cttgttttaa tgaagtaaaa gaataacggc actttttggt gaatttgcac
    17821 tccaagcaac gttattgaat aaccaaagga agtgacatga aatcgctgcg tttaatgtta
    17881 tgcgctatgc cattgatgct gaccggctgt tccacgatgt cgtcagttaa ctggtctgcc
    17941 gctaacccgt ggaactggtt tgggtcgtcc accaaagtga gcgagcaggg cgttggtgaa
    18001 ttaacggcgt ccacaccact gcaagaacaa gccattgccg atgcgcttga tggcgattat
    18061 cgcctgcgca gcggaatgaa aaccgcgaac ggcaacgtgg tgcgcttttt tgaagtgatg
    18121 aagggcgaca acgtggcgat ggtgattaac ggcgatcaag gcacgatcag tcgcattgac
    18181 gtgctggata gcgatattcc tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac
    18241 ctttacagca aagcatttgg caattgccaa aaagctgatg gtgatgataa tcgtgccgtc
    18301 gaatgtaaag ccgaaggcag tcaacatatt agctaccagt tcagcgggga atggagtggt
    18361 cctgaagggt taatgccttc ggacgatacc ctgaaaaact ggaaagtcag taaaattatc
    18421 tggcggcgtt aatttgcgtc tgaacaaacc gccgccgcga aaaaacacgt aaaatagcgc
    18481 tcaacaatgc cacggattgc gtggcattat tattttcagg aggaacaatg tctcaggttc
    18541 agagtggcat tttgccagaa cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag
    18601 gggaagttga cgccctgcgt gcggccagta aaacatttgc cgacaaactg gcaacttttg
    18661 aagcgaaatt cccggacgcg catcttggtg cggtggttgc ctttggtaac aacacctggc
    18721 gcgctctgag cggcggcgtt ggggcagaag agctgaaaga ttttccgggc tacggtaaag
    18781 gccttgcgcc gaccacccag ttcgatgtgt tgatccacat tctttctctg cgtcacgacg
    18841 taaacttctc tgtcgcccag gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag
    18901 aagagatcca cggcttccgt tgggttgaag agcgtgacct gagcggcttt gttgacggta
    18961 cggaaaaccc ggcgggtgaa gagacgcgtc gcgaagtggc ggttatcaaa gacggcgtgg
    19021 atgcgggcgg cagctatgtg tttgtccagc gttgggaaca caacctgaag cagctcaacc
    19081 ggatgagcgt tcacgatcag gagatgatga tcgggcgcac caaagaggcc aacgaagaga
    19141 tcgacggcga cgaacgtccg gaaacctctc acctcacccg cgttgatctg aaagaagatg
    19201 gcaaagggct gaagattgtt cgccagagcc tgccgtacgg cactgccagt ggcactcacg
    19261 gtctgtactt ctgcgcctac tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca
    19321 tgtttggcga taccgatggt aagcgtgatg cgatgttgcg tttcaccaaa ccggtaaccg
    19381 gcggctatta tttcgcaccg tcgctggaca agttgatggc gctgtaagcg tttctgattc
    19441 attgattaaa ggccaggcag taacattact gactggcctt tttattactt ttgctttaac
    19501 gccgcataca cctgatcaac tacccaacca tacgttgcaa tcggcagctg accgctttcg
    19561 aacatattgg gattcttttg cggatcggca accggcgaat gtggcttgtt gcttaacatc
    19621 acaatcgcca tatgattcac cggatcgata acggtcaccg ttccggtcca gccagtgtga
    19681 ccgtaggttt gcgggcttgc cagcgtgcca aacgtcggcg tcatggtggc attaccattc
    19741 acgcgccagc cgaggccaaa agtggcatct tccttagagc tggtggtgaa catcttcacc
    19801 gtttccgcac tgaacagctg cacatcgcca tagcccccgc cgttcagcat cgtttgcatt
    19861 aacaccgcaa tatcgccggt attggaaaac aaacctgcgt gcccggaaac gccgcccatc
    19921 gaataaaagg ctttttcatc gtgcacctga ccccagagag tggaggtgcg gatattcggg
    19981 aaatggatca cgccatcgcg ggtattgccg ttcaattccg tggcggcaat ttgctgcggt
    20041 ttaaagcctt tcagcagcgg gttaaatacc gtatgtgtca ggccgagcgg gcgataaatc
    20101 gactcttcaa catagcggtc aagcggctga ccggtgaccg actcgacgat aaatccaagc
    20161 agcatataat cgacatcact gtaaatatgc ttgctgccgg gctgatactc cagcggcgtg
    20221 cgcttgatca tctccagcgt ctggccttta tcctgggaat ataacgcgcc cgcgaccgct
    20281 ttattcggat attgtgggtc cgccgggaaa ccgccgctgt gatgcagcag gtcagaaatc
    20341 cgcagcgtgt ttttgccttt gatggtgtca tttggactat cggcaaaccc cggaatatat
    20401 ttcgcaatcc gatcgtcagg atgcagtttg ccttcgctca tcagcttttg cagggcgaag
    20461 ttcgtggcgt acattttggt gtttgaggcc aggtcataca gcgtcccggt ggtggctttg
    20521 acaggctgtt ccatcaacac gctgccatcg tactttttcg ccgctcccca ggctttgcga
    20581 tacacaatct gattatcttt aatgatcagc aggtttacgc cgggataacc ggcgtccact
    20641 tgctggctaa tccagcgatc catctggtta agccgttcga cgttgaaccc tgctttctct
    20701 ggcgaacttt ctgtcagaac agggtatttt gcggcgctga cgctacaact aacagccagc
    20761 agagaaagat agagcattgt ccgtttcatg atatcagtcc agattgacgt tacggcagcc
    20821 aaagagcgtg gtgaaaataa acccgcaaac ccatgccacc agaatcccgc cagcataaac
    20881 cgccattgcc ggaaggatgc cttgtgcgga agtcatcagc ggcagcgcca ccagaccaga
    20941 cggcccaaag gcgctgttta agcccatcgg cagaccccac caggctatca ggccgataaa
    21001 caaaccgccc gctgcgccgc ctaaacaggc ggtaataaac ggtttcatgc ggggcagggt
    21061 gacgccgtaa atcaacggtt caccaacgcc cagcagaccg ggaataatcg ccccgcgtac
    21121 ctgactgcgt aatgcactgt gcggttgcgc ccgccagtag agtgccagcg ccgcgcccac
    21181 ctggcccgcg cctgccattg aaaggatggg aaataagctg ttgaatccct ggctgtccat
    21241 taacgcgagg taaacaggaa taaagccctg atgcacgcca aataccacgg cgatcaggaa
    21301 cagcccggct aaaaccgcac aaccgaacgg attactgttc aggtgcataa acagccatga
    21361 cataccttcg aataaccagc cacccagcgg cataatgatc aggtacgcaa gcgtagcggt
    21421 gatcagcagg gtgattaacg aggtcagcag catgtcgaga tcgtccggca taaagcggcg
    21481 caccatgcct tcaatgcggg cgcaggccca cgcggcaatc agcacgccga taatattgcc
    21541 gcgcggatcg atgggcaggc caaagaaatc gtgaaaaccg gcgtagtaac cggtggttgc
    21601 agcggggtta taaccgagca aaaagagtgc ggcaataatt gcgccattga cgcccgtgcc
    21661 gccaaatgcc tgggcggcgt tatagcccac cagaatcacc aggaaagtga acaaaccttt
    21721 gctgaacacc ttcataaaat tcagcgcgtc gggtagcgtg ccctgagcat ctgccggaac
    21781 gtgcatcacc gtggcaatta acgtcgctat ccccagcagc agaccggcgg caataaaacc
    21841 ggggatcagc ggcgtgaaga tggtggcgaa tttggcgaga aattgttgca cgccggaggt
    21901 ttgtttggct tttaactgac gtttgttctg ggcggcgatt tctgcggcat cctgtaccgg
    21961 tgcctcgccc agcagctcgc tcatcgcttt tgcggcacga tgcgctttac caggcccaaa
    22021 aacgacctgc acctggtcgc tggtcaaaat gacgcccttc acgccttcca gcgttttgat
    22081 attgggatca accagtgaac tgtcatgtac acccagacgc aggcgcgtca tacagttacc
    22141 acaactggcg atatttcccg gtccgccgac acgggtaaga atggtgttca gaagttcact
    22201 gctgatctct ttggccatgg gttattcctt gtctaaaacc tgacgaataa agccgccgtg
    22261 ttgatccagg cgttttttag cttctgcggc atcgagattt ttcagcacca tcacaatggc
    22321 cgttttacag ttgcgctcgc aggcaattaa cgccgcttcc gcttgttcgg cattacatcc
    22381 ggtggcgttt ttaacgatat tgacctggcg cacatgcagt ttttcgttgg tggcgaccac
    22441 atcgaccatc aggttgccga acactttgcc agatttaatc atcagcccgg tggaaagcat
    22501 attgagcacc agtttctgcg ctgtgcctgc tttcattcgc gaagaaccag tgaccacttc
    22561 ggcacctaca atcggtgtaa tggcaaactc ggcggtggtg gaaacggcac tccccggatt
    22621 acaggaaatt cccactgtgc ggcagccgag ctggcgtgcg tattccagtc cggcaatcac
    22681 atacggcgtg cgaccgctgg cagcaatgcc aaccaccaca tcctgtgccg ttaaattaat
    22741 atttttcaga tcgttaacac caccttcccg gctatcttca gcgccttcca ccgcgtgctg
    22801 aatggcatat tcgcctccag caatcaaacc aaccaccaga cccggtttca cgccgtaggt
    22861 gggcggacat tcgctggcat ccagaatccc cagacgaccg gatgttcccg caccgagata
    22921 aatcagacga ccgccgccgc tgacctgggc gtggataaca tcgatcgccg cagcgatatc
    22981 cggcaggacg cgctcaacgg caagcggtac ggttttatct tcatcgttga taatccggca
    23041 catttccagc gtcgatacgc ggtcaatttc agccgaggcg gtgttcgagc cttcagtaat
    23101 catcttttct agttgcatcg gtggtgacct tactatttac gatcaaataa ttgcagatgt
    23161 atggaatcat atatcccgaa ttgacagact taaaggaatc attgagtcta cactcgcaga
    23221 gtgtgaagag cgtcataaga acttaagcgg aatttatgct gccagcccag gagagtggaa
    23281 gatgttgtac ctgacgaaaa taagcaatgc ggggagtgaa ttcaccgaaa acgagcaaaa
    23341 aatcgctgac tttctgcagg ccaacgtgag tgagctgcaa tccgtttctt cacggcagat
    23401 ggcgaaacag ctcggcatta gccagtcgag catagtgaaa tttgcccaaa aactcggcgc
    23461 gcaaggattt actgaattgc gtatggcgtt aataggcgaa tacagtgcca gccgggagaa
    23521 aacgaatgcc acggcgctgc acctgcacag ttcgattacc agtgatgatt cgctggaagt
    23581 gattgcccgc aaattgaatc gcgaaaaaga gctggcgctg gagcaaacct gttcgttgtt
    23641 cgactacgcg cggctgcaaa aaatcattga ggtcatcagt aaagcaccgt ttatccagat
    23701 aacgggcctg ggcggatcgg cgctggtggg gcgcgatctg tcgttcaaac tgatgaaaat
    23761 tggctatcgc gtggcctgcg aagccgacac gcacgtacaa gccaccgttt cccaggcgtt
    23821 gaaaaaaggc gatgtacaaa tcgctatctc ttacagcggc agtaaaaaag agattgtctt
    23881 atgcgcagaa gcggcacgaa agcagggggc aacggtgatt gccattactt ctctggctga
    23941 ttctccgctg cggcggctgg cgcatttcac gctcgatacc gtttccggtg aaaccgaatg
    24001 gcgtagctcg tcgatgtcta cccgcacggc gcaaaattct gttaccgatt tactgtttgt
    24061 tggtcttgtg caactcaatg atgtggagtc attgaaaatg attcagcgca gcagtgagct
    24121 aacacaacgc ctgaaatgat gcataaagca gcgactggat tgagattttc ctgaattagt
    24181 gagctgatcc gcagcaatat tttgtttatc ctgtattttc agagggaatg gagtgtaacg
    24241 ctctgtatta acaaggagag cattaaaatg ggtaaactca cgggcaagac agcactgatt
    24301 acgggcgcat tgcagggaat tggcgaagga attgccagaa cttttgcgcg tcatggcgca
    24361 aacctaatct tgctggatat ctcccctgag atcgaaaagc tggcggacga actgtgtggt
    24421 cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg acccggcgtc ggtagccgca
    24481 gctatcaaac gcgcgaagga aaaagaaggg cgcattgata tcctggtgaa taacgcaggc
    24541 gtttgtcgtc tgggcagttt cctcgatatg agcgatgacg atcgcgattt ccatattgac
    24601 atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc tgccggagat gattgcccgc
    24661 aaagatggtc gcattgtgat gatgtcttcg gtcactggcg atatggtggc cgatcctggc
    24721 gaaacggcgt acgccttaac gaaagcggcg attgttggcc tgacaaaatc gctggcggtg
    24781 gagtacgcgc agtctggtat tcgcgttaac gccatttgcc cgggatacgt gcgcacacca
    24841 atggcggaaa gcattgcccg ccagtcgaac ccggaagatc cagagtcggt gctgactgaa
    24901 atggcgaaag caatcccgat gcgtcgcctc gccgatccgc tggaagtcgg cgaactggcg
    24961 gccttcctcg catcggatga atccagctat ttaaccggta cacagaatgt gattgatggc
    25021 ggcagcacac tgccggagac ggttagcgtc ggtatctgat tcacctctgt ttcctccctg
    25081 catttgtggg gaggatttcg ttttaaaccc ggttcttatt cccttttcaa cttccaaatc
    25141 accaaacggt atataaaacc gttactcctt tcacgtccgt tataaatatg atggctatta
    25201 gaaagtcatt aaatttataa gggtgcgcaa tggccgttaa cttactgaaa aagaactcac
    25261 tcgcgctggt cgcttctctg ctgctggcgg gccatgtaca ggcaacggaa ctgctgaaca
    25321 gttcttatga cgtctcccgc gagctgtttg ccgccctgaa tccgccgttt gagcaacaat
    25381 gggcaaaaga taacggcggc gacaaactga cgataaaaca atctcatgcc gggtcatcaa
    25441 aacaggcgct ggcgatttta cagggcttaa aagccgacgt tgtcacttat aaccaggtga
    25501 ccgacgtaca aatcctgcac gataaaggca agctgatccc ggccgactgg cagtcgcgcc
    25561 tgccgaataa tagctcgccg ttctactcca ccatgggctt cctggtgcgt aagggtaacc
    25621 cgaagaatat ccacgattgg aacgacctgg tgcgctccga cgtgaagctg attttcccga
    25681 acccgaaaac gtcgggtaac gcgcgttata cctatctggc ggcatggggc gcagcggata
    25741 aagctgacgg tggcgataaa gccaaaaccg aacagtttat gacccagttc ctgaaaaacg
    25801 ttgaagtgtt cgataccggc ggtcgtggcg caaccaccac ttttgccgag cgtggcctgg
    25861 gcgatgtgct gattagcttt gaatcggaag tgaacaacat ccgtaaacag tatgaagcac
    25921 agggctttga agtggtgatc ccgaaaacca atattctggc ggaattcccg gtggcgtggg
    25981 tcgataaaaa tgtgcaggcc aacggtacgg aaaaagccgc aaaagcctac ctgaactggc
    26041 tctacagccc acaggcgcag accatcatca ccgactatta ctatcgtgtg aataacccgg
    26101 aagtcatgga caaactgaaa aacaaattcc cgcagaccga gctgttccgc gtggaagaca
    26161 aatttggctc ctggccggaa gtgatgaaaa cccacttcac cagcggcggc gagttagaca
    26221 agctgttagc ggcggggcgt aattaatgtt tgcagtttca tcaagacgcg tgctgccggg
    26281 ctttacctta agcctcggca ccagtctgct gtttgtgtgc ctgattttgc tgctgccgct
    26341 ctccgcgctg gtgatgcagc tggctcagat gagctgggcg cagtactggg aggtgatcac
    26401 caacccgcag gtagtagcgg cctacaaagt aacgctgctg tcagcgtttg tggcatcgat
    26461 ttttaacggc gtttttggcc tgctgatggc gtggatctta acccgttatc gctttccggg
    26521 ccgcacgctg cttgatgcgc tgatggattt accctttgcg ctgccaacgg cagtcgccgg
    26581 tttaacgctg gcctcgctct tttccgtaaa cggtttttac ggtgaatggc tggcgaagtt
    26641 tgatatcaaa gtcacctata cctggcttgg tattgcggtg gcgatggcct ttaccagcat
    26701 tccgtttgtg gtgcgtaccg tgcagccggt gctggaagag ttaggcccgg aatatgaaga
    26761 agcggcggaa acgcttggtg caacgcgctg gcagagtttc tgcaaagtgg tgctgccgga
    26821 actttctcca gcgctggtgg cgggcgtggc gttgtcgttt acccgtagtc ttggtgagtt
    26881 tggcgcggtg atatttatcg ccgggaacat cgcctggaag acggaagtga cgtcgctgat
    26941 gatttttgtg cgcttgcagg agtttgatta cccggcggcg agcgcgattg cttcggtgat
    27001 cctcgcggca tccctgctgc tgctgttctc aattaacact ctgcaaagtc gctttggtcg
    27061 gcgtgtggta ggtcattaat ggcggaagtt acccaattga agcgttatga cgcgcgcccg
    27121 attaactggg gcaaatggtt tctaattggc atcgggatgc tggtttcagc gttcatcctg
    27181 ctggtgccga tgatttacat cttcgtgcag gcattcagca aggggctgat gccggtttta
    27241 cagaatctgg ccgatccgga catgctgcac gccatctggc tgacggtgat gatcgcgctg
    27301 attgccgtac cggtaaacct ggtgttcggc attctgctgg cctggctggt gacgcgcttt
    27361 aacttccctg gacgccagtt actgctgacg ctactggaca ttccgtttgc cgtatcgccg
    27421 gtggttgccg gtctggtgta tctgctgttc tacggctcta acggcccgct gggcggttgg
    27481 ctggatgaac ataatctgca aattatgttc tcctggccgg gaatggtgct ggtcaccatc
    27541 ttcgtgacgt gtccgtttgt ggtgcgcgaa ctggtgccgg tgatgttaag ccagggcagt
    27601 caggaagacg aggcggcgat tttgcttggc gcgtctggct ggcagatgtt ccgtcgtgtc
    27661 acattgccga acatccgctg ggcgctgctt tatggcgtag tgctgaccaa cgcccgcgcg
    27721 attggcgagt ttggcgcggt gtcggtggtt tccggctcga ttcgcggcga aacactttcg
    27781 ctgccgttac agattgaatt gctggagcag gactacaaca ccgtcggctc ctttaccgct
    27841 gcggcactgt taacgctgat ggcgattatc accctgtttt taaaaagtat gttgcagtgg
    27901 cgcctggaga atcaggaaaa acgcgcacag caggaggaac atcatgagca ttgagattgc
    27961 caatattaag aagtcgtttg gtcgcaccca ggtgctgaac gatatctcac tggatattcc
    28021 ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc gggaaaacca cgctgctgcg
    28081 cattatcgcc gggctggagc atcaaaccag cgggcatatt cgcttccacg gcaccgacgt
    28141 cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg ttccagcatt acgcgctgtt
    28201 ccgtcatatg acggtgttcg acaatatcgc ttttggcctg acggtgctgc cgcgtcgcga
    28261 gcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa ttgctggaga tggtgcagct
    28321 tgctcatctg gcagatcgtt atccggcaca gctttccggc ggccagaaac agcgcgtggc
    28381 gctggcgcgc gcgctggctg tagaaccgca aattctgctg cttgatgaac cgtttggcgc
    28441 gctggatgcg caggtgcgta aagagctgcg tcgctggctg cgtcaactgc atgaagagct
    28501 gaaattcacc agcgtgttcg tgacccacga ccaggaagaa gcgacggaag tagctgatcg
    28561 tgtagtggtg atgagccagg gcaatattga acaggctgat gcgccggagc aggtatggcg
    28621 cgaaccagcg acccgttttg tgctcgaatt tatgggcgaa gtgaaccgcc tgcagggaac
    28681 cattcgcggc gggcagttcc atgttggcgc gcatcgctgg ccgctgggct acacacctgc
    28741 gtatcagggt ccggtggatc tcttcctgcg cccgtgggaa gtggatatca gccgccgtac
    28801 cagtctcgat tcgccgctgc cggtacaggt actggaagcc agcccgaaag gtcactacac
    28861 ccaattagtg gtgcagccgc tggggtggta caacgaaccg ctgacggtcg tgatgcatgg
    28921 cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt ctgcaacatg cgcggctgta
    28981 taacggcgac gagcgtatcg aaacccgaga tgaggaactt gctctcgcac aaagcgcctg
    29041 ataggttgag tgaatgttaa acgcccggag gcgcttcccg cgatccgggc tttttaatgg
    29101 caaggtttgt aacctgtaga cctgataaga cgcgcaagcg tcgcatcagg caacaccacg
    29161 tatggataga gatcgtgagt acattagaac aaacaatagg caatacgcct ctggtgaagt
    29221 tgcagcgaat ggggccggat aacggcagtg aagtgtggtt aaaactggaa ggcaataacc
    29281 cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat cgtcgaggcg gaaaagcgcg
    29341 gggaaattaa accgggtgat gtcttaatcg aagccaccag tggtaacacc ggcattgcgc
    29401 tggcaatgat tgccgcgctg aaaggctatc gcatgaaatt gctgatgccc gacaacatga
    29461 gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga actgattctt gtcaccaaag
    29521 agcagggcat ggaaggtgcg cgcgatctgg cgctggagat ggcgaatcgt ggcgaaggaa
    29581 agctgctcga tcagtttaat aatcccgata acccttacgc ccattacacc actaccgggc
    29641 cggaaatctg gcagcaaacc ggcgggcgca ttacccattt tgtctccagc atgggaacaa
    29701 ccggcactat caccggcgtt tcacgcttta tgcgcgaaca atccaaacca gtaacgattg
    29761 ttggcctgca gccggaagaa ggcagcagta ttcccggcat tcgccgctgg cctgcggaat
    29821 atctgccggg gattttcaac gcttctctgg tggatgaggt gctggatatt catcagcgcg
    29881 atgcagaaaa caccatgcgc gaactggcag tgcgggaagg aatattctgt ggcgtcagct
    29941 ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc taaccctggt gcggtggtgg
    30001 tagcgatcat ctgcgatcgt ggcgatcgct acctttccac cggggtgttt ggggaagagc
    30061 attttagcca gggggcgggg atttaagatt attgcgcagg caccgtttta tccgtctgcg
    30121 caattagcgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg atcttcacaa
    30181 tatacggctt cccggtaatc gatgacgacg aagcaacctt atcaataaac tgctcggcag
    30241 tgtcaatgcg gttacgggta ttgcccagct tcagacgcag atgagaaacc gcttcatcgc
    30301 aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct aatccctcca
    30361 gcatggcgtt gatgcgggct tcttcatggg cagttaactt cgcgtaagcg gggagtgtca
    30421 ttagtagtgt aatgaccaga caaatgattt tcttcattga attaccttat ccttgttttg
    30481 tgatagccga tttgactgcc acaatcaggt aaagtttcgg ttggaatacg atgaggatca
    30541 tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa gagcagggcg
    30601 ctgcaggcgg atatcgtcgc cgtgcagtcg caggtggttt acggcagcgt gggcaacagc
    30661 attgccgtgc ctgctatcaa acagaacggt ctgaatgttt ttgccgtgcc gacggtattg
    30721 ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga tgaatggttt
    30781 agcggttatt tgcgtgcgct tcaggagcgt gatgcgctgc gccagctgcg cgctgtaacc
    30841 acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct gactgcgcta
    30901 cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga tattgatagc
    30961 ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact gccgctggcg
    31021 cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa ttgccgcgat
    31081 ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa atgggtggtg
    31141 attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt ggtcagtgcc
    31201 gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg gactggcgac
    31261 ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa cgatgcggtg
    31321 caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca tgagagcgat
    31381 gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg cgccattttt
    31441 cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac ggttacgcta
    31501 ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac cggagtcagg
    31561 gtagacttgg ctttctcttc cagtagcggc agatcaaatt caatgacagt atcgccaact
    31621 ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag ttcaacggtg
    31681 tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga gaacgcgtga
    31741 ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt gttacccgtt
    31801 ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc cggcacgtct
    31861 tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc ggtatccttc
    31921 ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt ctcctaagca
    31981 gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga acttgttaac
    32041 cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta acaccttcgc
    32101 atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa tggcgctcat
    32161 agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat cgccagcaag
    32221 ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa cttgcttgat
    32281 caagttcagc acggacggtg acattggctg gtaaaggtgt gaaatcatat cattaccacg
    32341 gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga aatcaacttc
    32401 tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta cgccgatttc
    32461 aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga tttcgatctc
    32521 tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa tgcgcaattt
    32581 accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct ctttacgatc
    32641 catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg ggaagttcat
    32701 gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg cctgcgagcc
    32761 acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg gcagcgcgtc
    32821 gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc cgttacgctc
    32881 tgcaccttca acgtcacgaa ccgtaccaat gttagcgcac acttctacct ggtgaccgtc
    32941 cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag aagccacttg
    33001 ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt aaacctgatt
    33061 atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga cgctaccggt
    33121 acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg aagtacggcc
    33181 acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg tttcggacgg
    33241 cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt cgataatctt
    33301 caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag ccgcacgttc
    33361 tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga taacttcatg
    33421 agcagctgcg tcagctgtca tgtgcttatc tttaatcagg gctatgattt cctgctccag
    33481 ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt cttcaccgaa
    33541 cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg cacgaccgct
    33601 cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa atttttttcc ggtcaatgac
    33661 aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg atgctaaaat
    33721 gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc gggaaattac
    33781 tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc ttcgccttct
    33841 gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt aaacaggctt
    33901 ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa gcccttagct
    33961 tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt aatggtaact
    34021 tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa ctaaagtcta
    34081 gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga cgcgacgcac
    34141 gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc gtacgtcatt
    34201 aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc agctttggct
    34261 tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat accagttccg
    34321 acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa aaagcaccta
    34381 aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcagt tctttctcag
    34441 tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgac ggtagaataa
    34501 ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgcc gcagcaacag
    34561 ctgctccaga agagatacct gcaagaatac cttcttcttc catcagacga cgcgcggtag
    34621 aaatcgcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga tcgaggttag
    34681 ctgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta atctcttcac
    34741 cagccagtgc ctgggcgata actggagaat cggttggctc aacggcgaca gagataagat
    34801 cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtcagcgta ccgccagtcc
    34861 caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc tccggaccgg
    34921 tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc agcaggtatt
    34981 tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc atgcctttag
    35041 caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg cgttcaatac
    35101 tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct acataggcca
    35161 gtgcaatccc ggtattaccg ctggtcggtt caaccagttc aacgcctggt ttcagcacgc
    35221 cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg ctgaagctgg
    35281 ggttacgaga ttccaccttc gccagaatgc gtccgttacc gatgcgattc aggcgaacca
    35341 gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg gcctgtcctt
    35401 aactgtatga aattgggata caacaggtag catacccgct cagagaatat gcggaagtaa
    35461 ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga ataaggggcg
    35521 gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga ttgttaccgc
    35581 cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc acaaacggca
    35641 acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt taaagcacca
    35701 aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt aaacggcact
    35761 ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc gctaaacagg
    35821 aaccacagta ccggcgcgac ggtttgccca ataccaggga ttaagtaaag aattagcaat
    35881 acaattgcgc gcggcagata ccaggcaaat ttttgccatt cgcgtttcat gattcgcggc
    35941 acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt caatcgtgct
    36001 tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc aatcgtggag
    36061 aagaaagagc caaacactaa cagcacagag atgacagcca gaggccacaa cagataactc
    36121 agccattgta gccagtccgg aacgtaactc atgagagtcg ggatccagac atcgagctgt
    36181 gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag cggtaaaata
    36241 acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa atagtaaaaa
    36301 ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc cttttgacca
    36361 atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt taggatatgt
    36421 tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag ttaatagtgc
    36481 acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc gtggtggcaa
    36541 ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt aatcattgtt
    36601 ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg taaagaacga
    36661 tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga cgacgattct
    36721 tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca ccgcgtgaat
    36781 catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca acaccagtac
    36841 caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccgcc tgaagcgcag
    36901 gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc agcagcctgc ctatcagccg
    36961 cagcctgaac agccgttgca gcagccagtt tcgccacagg tcgcgccagc gccgcagcct
    37021 gtgcattcag caccgcaacc ggcacaacag gctttccagc ctgcagaacc cgtagcggca
    37081 ccacagcctg agcctgtagc ggaaccggct ccagttatgg ataaaccgaa gcgcaaagaa
    37141 gcggtgatta tcatgaacgt cgcggcgcat cacggtagcg agctaaacgg tgaactgctt
    37201 cttaacagca ttcaacaagc gggcttcatt tttggcgata tgaatattta ccatcgtcat
    37261 cttagcccgg atggcagcgg cccggcgtta ttcagcctgg cgaatatggt gaaaccggga
    37321 acctttgatc ctgaaatgaa ggatttcact actccgggtg tcaccatctt tatgcaggta
    37381 ccgtcttacg gtgacgagct gcagaacttc aagctgatgc tgcaatctgc gcagcatatt
    37441 gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc gtatgatgac tccgcagaaa
    37501 ttgcgcgagt accaggacat catccgcgaa gtcaaagacg ccaacgcctg atacacttaa
    37561 ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt ttttagcatt gatggtgcga
    37621 tatggaatca atcgaacaac aactgacaga actgcgaacg acgcttcgcc atcatgaata
    37681 tctttatcat gtgatggatg cgccggaaat tcccgacgct gaatacgaca ggctgatgcg
    37741 cgaactgcgc gagctggaaa ccaaacatcc agaactgatt acgcctgatt cgcctaccca
    37801 acgtgtaggc gctgcgccgc tggcggcttt cagccagata cgccatgaag taccaatgct
    37861 gtcactggat aacgtttttg atgaagaaag ctttcttgct ttcaacaaac gtgtgcagga
    37921 ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag ctgaagctgg atggccttgc
    37981 cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc gcgacccgtg gcgatggcac
    38041 caccggggaa gatatcacgt ctaatgtgcg tactattcgc gctattccgc tgaagctgca
    38101 cggagagaat atcccggcgc gtctggaagt gcgtggtgaa gtgttcctgc cgcaggcggg
    38161 gttcgaaaag attaacgaag atgcgcgacg cacgggcggg aaagtttttg ctaacccacg
    38221 taatgcggca gctggttcac tgcgtcagct tgatccgcgt attacagcga agcgaccgct
    38281 cacttttttc tgctatggcg ttggtgttct ggaaggtggc gagctgccgg atactcatct
    38341 tggccgttta ctgcaattta aaaagtgggg gttgccggtc agcgatcggg taacgctttg
    38401 tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg gaagaagacc gcccgacgct
    38461 gggctttgat atcgacggcg tggtgattaa ggtcaactca ctggaacagc aggagcagct
    38521 tggctttgtc gcgcgtgccc cgcgctgggc ggtagcgttt aaattcccgg cgcaggagca
    38581 gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt actggcgcga ttacgcctgt
    38641 tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg agtaacgcaa ccttacacaa
    38701 tgcggatgaa atcgaacgtc ttggtttacg cattggcgat aaagtggtga ttcgccgcgc
    38761 tggcgacgtg atcccgcagg tggttaacgt cgtgctttct gaacgcccgg aagatacccg
    38821 tgaggttgta ttcccgacgc attgtccggt atgtggttct gacgttgagc gtgtggaagg
    38881 tgaagcggtt gcccgctgta ccggtggcct gatttgcgga gcgcagcgta aagagtcgct
    38941 gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga atgggcgaca aaatcatcga
    39001 tcagctggtt gaaaaagaat atgtccatac tccggcagat ctgttcaaac tcaccgcagg
    39061 caaactgacc ggactggagc gtatggggcc aaaatcggca caaaacgtgg ttaacgcgct
    39121 agaaaaagcg aaagaaacca cctttgctcg cttcctctat gcacttggca tccgtgaagt
    39181 cggcgaggcc accgcagcag gtctggcggc atatttcggc acgctggaag cgctggaagc
    39241 cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc attgtcgttg catcccacgt
    39301 tcacaacttc tttgccgaag aaagcaaccg caatgtcatc agcgagctgt tggcggaagg
    39361 tgttcactgg cctgcgccga tcgttatcaa cgcggaagag attgacagcc cgtttgctgg
    39421 taaaaccgtg gtgcttacgg gcagcttaag ccagatgtcg cgtgatgacg ctaaagctcg
    39481 actggttgaa ctgggcgcga aagtcgcggg cagcgtgtcg aagaaaaccg atctggtgat
    39541 tgccggagaa gcggcaggtt ctaaactggc gaaagcgcag gaactgggca ttgaagtcat
    39601 cgacgaagcg gaaatgctgc gtttgctggg tagctgagat ggaaaaagag cagctgattg
    39661 aaatagccaa tacgataatg ccgtttggca aatataaagg gcgtcgctta atcgacctgc
    39721 cggaggagta tctgctgtgg tttgcccgca aagatgaatt cccggcaggg aagctcggtg
    39781 agctaatgca aatcacgctg ctgattaaaa ccgaggggct gacgcaactg gtccagcccc
    39841 tgaagcgtcc gctttaagct ttatcggcgc tgctttcctg ctgtgcctgt aactgttcgg
    39901 tctggcgttt gtatcgacgc gccagcaccg cacagaccat caattggatc tgatggaaaa
    39961 tcattagggg cagcaccatc ataccgatca ccgatgtggg gaacagaatg tttgccatcg
    40021 ggatgccatt tgccagactc tttttcgaac cacaaaagac gatagtaatt tcatctgcct
    40081 tattgaagcc cagtcggcgt gccataaaga cgttaactac aatcacgata gccagaagaa
    40141 cgcaactgac caccacgata aacagcaatg atccccagcc aactttatgc cagataccat
    40201 taacgacggc ttcgctgaac gctgtataaa ccaccaacag aatggacgtc tggtcagttt
    40261 tcgcaatcca ttttttattg cgcgacaccc agtcaccaat ccacggccgg gaaagatgcc
    40321 ccaacacaaa cggcagcagc agttgcagca taattttacc gacctgctca aggctgcccc
    40381 ctgcaccgtg aacattcatc accagaccaa ccagcaatgg tgaaaggaaa atccccagca
    40441 ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt accgcccgcc attgacgtga
    40501 aggcgattgc agactgcacg gtagccggga gaatgcacaa gtagagaaaa ccggagtaga
    40561 gcatcgggtc gacatttacc ggtttccacc aggcaaacag tacacccaga atcggaaaca
    40621 gcacgaaggt gctgcaaatt acccacaaat gcagtcgcca gtgaccaccg ccagcaataa
    40681 tcgcctcacg cgacaacttc gcgccgtgca taaagaacag cagggcaatc gctgcggtgg
    40741 tcagattttc aaagaagggg acgaaatcgc ctctggccgg aaagaaagag gccagcaaca
    40801 ccaccgtgat cagggttaag gtgaaaggat cgaggatacg aaaaagtttc ataaagactc
    40861 cagatgatcg atggttctat tgtgcttctt ccagtttgag aaataaaatt gatttattgc
    40921 atccattcat gaagaaaaca gatgaattat tctttaaaac aattaaaggt tttcgtcaca
    40981 gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta tcggcctgag ccagtcggca
    41041 gtgagtcaca gtgtgaagga actggaaaat catactggtg ttcgcctgct ggacagaacc
    41101 acgcgtgaag tggtgcttac agatgcaggg cagcagctgg ctttgcgtct tgagcgactg
    41161 ctggatgaac tgaacagcac gttgcgcgat accgggcgta tggggcaaca actgagcgga
    41221 aaagttcggg tcgctgccag ccagaccatt tccgcgcatc ttattccgca atgcattgct
    41281 gaaagccatc gccgctatcc agatattcag tttgtcctgc acgatcgtcc gcagcagtgg
    41341 gtgatggaaa gtattcgcca gggagatgtc gactttggca tcgtcatcga tccaggccca
    41401 gtgggcgatc tgcaatgtga agcgattctt tccgagcctt tctttctgct ttgccatcgc
    41461 gatagcgctt tggccgtcga agattatgta ccctggcagg cattacaggg agcgaaattg
    41521 gtgttgcagg attacgcgtc aggcagccga ccgctgattg acgcagcgct ggcgcgcaat
    41581 ggtattcagg cgaatattgt gcaggagatt ggacatccgg cgacgctgtt cccgatggta
    41641 gccgcaggca tcggcatcag tattcttccc gcactggcgc taccattgcc tgaaggtagc
    41701 ccactggtgg taaagcgcat tacgccggtt gttgaacgcc agttgatgct ggtgcgcagg
    41761 aagaaccgtt cactttccac cgccgctgaa gcgttatggg acgtggtacg agaccagggc
    41821 aatgcgctaa tggctgggcg ggaaggggat ccgttgtatc agatatagag tgaattcata
    41881 ctgccgggag ccccggcagt aatcaatcag aagttaaatt tgatggagta gattatcgca
    41941 tcctggtaaa aatcttcgcc cagtttgtca tccgcatatc gatactgaat ccccgtagta
    42001 atgtgtgatg ttgcattcca ccacagtgca actgcgccat tcagcccttc tttaccgcca
    42061 ttacccgctg cataggtagc atcacggtca aactcgtact cgttccagtt ggtaagagtg
    42121 aatttctcgc tgcccagcat aaagttgtaa cctgcaaccc agccggcgac atagccgttg
    42181 tcgccagtgt aataggtttg atctgtataa cgcttagcaa agaacggttt gaaccaccaa
    42241 ccgctgccgg tgaaattgta gccgataccg tacaggaaca tatcgtcgtg gaaattcacg
    42301 cgattcgcag aaccgtaggt gccatacgca tgcaagtaga gattaaatcc ggtgtcaccc
    42361 aggtaaatac ggttggtatt tttaaaggta taacgctgct cgctacctgg tttattatgg
    42421 cgaccgttat agaagttttc ccagtcgaag aacccgtaca tttctcccca actaaagtta
    42481 gcgccacctt caaattcaag ataaccaaaa tcatctttgt gtgatttggt cgacgatttt
    42541 tcggtagtcc ggctggtcca gtccagataa tgcacgccga tatctgcaaa accgccttta
    42601 aattctgcat gagatacaac aggtatcgct aatatagagg aaagtgtcag agttaaaaga
    42661 tgttttttca tagcataatt ccctatgccg atcgttatta gtagattgct taataaagtg
    42721 gtttgctaaa aacgacatat tgttaatgca aaggaattaa tatcgccaat acagttttta
    42781 ccgtggatta atagagaaaa gatataaatc catcgatatc gaatcgctat tgaatgcgtg
    42841 agaaaagtgt attggtaaga gccgggacaa cctcgctata aaaaggcgtt agattccacc
    42901 ctacagaaaa aggatatgta tgtctcaggt tcaattttct cataacccgc tgttttgcat
    42961 agatattatc aagacttata aacctgattt cacgccacga gtggccttta ttttaggttc
    43021 cgggctcggc gcgctggccg atcagattga gaacgctgtc gcaatttcct acgaaaagct
    43081 gccgggcttc ccggtaagta cggtacatgg tcatgcgggg gagttagtgc tgggccatct
    43141 ccagggcgta ccggtggtat gtatgaaagg tcgcggacat ttctacgaag gtcgtggaat
    43201 gaccattatg actgacgcaa ttcgtacctt taagctgctg ggctgcgagc tactgttctg
    43261 caccaatgcg gcaggctcgc tgcgtccgga agtgggggca ggcagcctgg tcgcattgaa
    43321 agatcatatc aacaccatgc ccggtacgcc gatggtgggt cttaacgatg atcgttttgg
    43381 agagcgcttc ttctcactgg caaatgccta cgatgcggaa taccgcgcac tgttacaaaa
    43441 agtggcgaaa gaagagggtt tccctctgac ggagggcgtg ttcgtctcgt atccggggcc
    43501 gaatttcgag actgcggcgg aaattcgcat gatgcaaatt attggtgggg atgttgttgg
    43561 tatgtctgtg gtgcctgagg ttatttcagc tcgccattgc gaccttaaag tcgttgcggt
    43621 ctctgcgatt accaatatgg cggaaggttt aagcgatgtg aaattgtcac atgcgcaaac
    43681 gctggcggct gcggagctct ctaagcagaa cttcatcaac cttatttgtg gctttctgcg
    43741 caaaattgcc tgagaaaaac aataagaaac ggcctcgcgg gtgaggccaa atgatgcgac
    43801 aaggaaacga ttatgagcat cgcgatgcgc ttaaaggtaa tgtccttttt gcaatatttt
    43861 atctggggga gctggctggt taccgtcggc tcttacatga ttaataccct tcatttcacc
    43921 ggcgctaatg ttggcatggt ttacagttcc aaagggatcg ccgcgattat tatgcctggt
    43981 ataatgggga tcatcgcaga caaatggctg cgcgcagaac gtgcatacat gctgtgtcac
    44041 ctggtgtgtg cgggcgtact tttttatgcg gcatccgtaa ctgatccgga tatgatgttt
    44101 tgggtgatgt tagtcaatgc gatggcgttt atgccgacta ttgcgttatc gaacagcgtc
    44161 tcttattcct gtcttgccca ggcagggctt gacccggtga ccgctttccc gcccattcgc
    44221 gtttttggta cggtggggtt cattgtcgcg atgtgggcag taagcctgct gcatctggaa
    44281 ttgagtagtc tgcagctgta tatcgcgtcc ggtgcgtcat tgctgctgtc ggcttatgcg
    44341 ctgactttgc cgaagattcc ggttgcggag aaaaaagcga ccacatcgct tgccagcaag
    44401 ctgggtctgg atgccttcgt gctgtttaaa aatccacgca tggccatctt tttcctcttt
    44461 gccatgatgc tgggtgcggt actgcaaatt accaacgttt ttggtaatcc gttcctacat
    44521 gatttcgccc gtaacccgga gtttgctgac agttttgtgg tgaaatatcc ctccatttta
    44581 ctgtcagttt cacagatggc agaagtgggc tttatactga ctatcccatt ctttttaaag
    44641 cgatttggca ttaaaaccgt catgctgatg agtatggtgg cctggacgct gcgctttggc
    44701 ttcttcgcct atggcgatcc gtcaacaacc ggatttattt tgctgctgct gtcgatgatt
    44761 gtttatggct gtgcattcga tttcttcaat atttctggtt cggtatttgt cgaacaggaa
    44821 gttgattcca gcattcgtgc cagcgcgcag gggctcttta tgaccatggt aaatggtgtc
    44881 ggcgcatggg ttggctcgat tctgagtggc atggcagtag attacttttc ggtggatggc
    44941 gtaaaagact ggcaaactat ctggctggtg tttgcaggat atgctctttt tctcgcagtg
    45001 atatttttct ttgggtttaa atataatcat gaccctgaaa agataaagca tcgagcggtg
    45061 gctcattaaa aggattcgca gctctgctct tcagagctgc ttttatgata aaggttaatt
    45121 agtgaaagat attttatttt cttccggcgt tggttttggc attggtgctt tgtttacaat
    45181 cgtgcgcttg cctattcctg taccaaatgt
//
LOCUS       DAPHDX010000021        44435 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPHDX010000021 DAPHDX010000000
VERSION     DAPHDX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.021"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(68..1432)
                     /gene="mnmE"
                     /locus_tag="RH058_001125"
     CDS             complement(68..1432)
                     /gene="mnmE"
                     /locus_tag="RH058_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV3136745.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1538..3184)
                     /gene="yidC"
                     /locus_tag="RH058_001126"
     CDS             complement(1538..3184)
                     /gene="yidC"
                     /locus_tag="RH058_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV3136746.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3187..3444)
                     /gene="yidD"
                     /locus_tag="RH058_001127"
     CDS             complement(3187..3444)
                     /gene="yidD"
                     /locus_tag="RH058_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV3136747.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3408..3767)
                     /gene="rnpA"
                     /locus_tag="RH058_001128"
     CDS             complement(3408..3767)
                     /gene="rnpA"
                     /locus_tag="RH058_001128"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV3136748.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3784..3924)
                     /gene="rpmH"
                     /locus_tag="RH058_001129"
     CDS             complement(3784..3924)
                     /gene="rpmH"
                     /locus_tag="RH058_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV3136749.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4154..4234)
                     /locus_tag="RH058_001130"
     CDS             complement(4154..4234)
                     /locus_tag="RH058_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136750.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4531..5934
                     /gene="dnaA"
                     /locus_tag="RH058_001131"
     CDS             4531..5934
                     /gene="dnaA"
                     /locus_tag="RH058_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV3136751.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5939..7039
                     /gene="dnaN"
                     /locus_tag="RH058_001132"
     CDS             5939..7039
                     /gene="dnaN"
                     /locus_tag="RH058_001132"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV3136752.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQQHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7039..8112
                     /gene="recF"
                     /locus_tag="RH058_001133"
     CDS             7039..8112
                     /gene="recF"
                     /locus_tag="RH058_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV3136753.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8141..10555
                     /gene="gyrB"
                     /locus_tag="RH058_001134"
     CDS             8141..10555
                     /gene="gyrB"
                     /locus_tag="RH058_001134"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV3136754.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10795..11193
                     /locus_tag="RH058_001135"
     CDS             10795..11193
                     /locus_tag="RH058_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV3136755.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11308..12120
                     /gene="yidA"
                     /locus_tag="RH058_001136"
     CDS             11308..12120
                     /gene="yidA"
                     /locus_tag="RH058_001136"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV3136756.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12166..12822)
                     /locus_tag="RH058_001137"
     CDS             complement(12166..12822)
                     /locus_tag="RH058_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136757.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13100..13789
                     /gene="dgoR"
                     /locus_tag="RH058_001138"
     CDS             13100..13789
                     /gene="dgoR"
                     /locus_tag="RH058_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDV3136758.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13786..14664
                     /gene="dgoK"
                     /locus_tag="RH058_001139"
     CDS             13786..14664
                     /gene="dgoK"
                     /locus_tag="RH058_001139"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDV3136759.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14648..15265
                     /gene="dgoA"
                     /locus_tag="RH058_001140"
     CDS             14648..15265
                     /gene="dgoA"
                     /locus_tag="RH058_001140"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV3136760.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15262..16410
                     /gene="dgoD"
                     /locus_tag="RH058_001141"
     CDS             15262..16410
                     /gene="dgoD"
                     /locus_tag="RH058_001141"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDV3136761.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16485..17822
                     /locus_tag="RH058_001142"
     CDS             16485..17822
                     /locus_tag="RH058_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3136762.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17819..18883)
                     /gene="cbrA"
                     /locus_tag="RH058_001143"
     CDS             complement(17819..18883)
                     /gene="cbrA"
                     /locus_tag="RH058_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV3136763.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRNIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            18984..20198
                     /locus_tag="RH058_001144"
     CDS             18984..20198
                     /locus_tag="RH058_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV3136764.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20200..20532)
                     /locus_tag="RH058_001145"
     CDS             complement(20200..20532)
                     /locus_tag="RH058_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV3136765.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20772..20844
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20838..21251
                     /gene="ibpA"
                     /locus_tag="RH058_001146"
     CDS             20838..21251
                     /gene="ibpA"
                     /locus_tag="RH058_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV3136766.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21363..21791
                     /gene="ibpB"
                     /locus_tag="RH058_001147"
     CDS             21363..21791
                     /gene="ibpB"
                     /locus_tag="RH058_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV3136767.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21988..23649
                     /locus_tag="RH058_001148"
     CDS             21988..23649
                     /locus_tag="RH058_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV3136768.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23646..24362)
                     /locus_tag="RH058_001149"
     CDS             complement(23646..24362)
                     /locus_tag="RH058_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3136769.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24658..26274
                     /locus_tag="RH058_001150"
     CDS             24658..26274
                     /locus_tag="RH058_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3136770.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGLAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLHEQLDSLINSHQSAENVAITEAV"
     gene            26274..27088
                     /locus_tag="RH058_001151"
                     /pseudo
     CDS             26274..27088
                     /locus_tag="RH058_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27085..27978)
                     /locus_tag="RH058_001152"
     CDS             complement(27085..27978)
                     /locus_tag="RH058_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3136771.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28139..29854
                     /locus_tag="RH058_001153"
     CDS             28139..29854
                     /locus_tag="RH058_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV3136772.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29851..31344
                     /locus_tag="RH058_001154"
     CDS             29851..31344
                     /locus_tag="RH058_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV3136773.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31391..31840)
                     /locus_tag="RH058_001155"
     CDS             complement(31391..31840)
                     /locus_tag="RH058_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136774.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31950..32297
                     /locus_tag="RH058_001156"
     CDS             31950..32297
                     /locus_tag="RH058_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV3136775.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32287..32649
                     /locus_tag="RH058_001157"
     CDS             32287..32649
                     /locus_tag="RH058_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV3136776.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32646..33143
                     /locus_tag="RH058_001158"
     CDS             32646..33143
                     /locus_tag="RH058_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV3136777.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33151..34335)
                     /gene="emrD"
                     /locus_tag="RH058_001159"
     CDS             complement(33151..34335)
                     /gene="emrD"
                     /locus_tag="RH058_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV3136778.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(34754..34843)
                     /gene="tisB"
                     /locus_tag="RH058_001160"
     CDS             complement(34754..34843)
                     /gene="tisB"
                     /locus_tag="RH058_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV3136779.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35407..35505
                     /gene="ivbL"
                     /locus_tag="RH058_001161"
     CDS             35407..35505
                     /gene="ivbL"
                     /locus_tag="RH058_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV3136780.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35611..37299
                     /gene="ilvB"
                     /locus_tag="RH058_001162"
     CDS             35611..37299
                     /gene="ilvB"
                     /locus_tag="RH058_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV3136781.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37303..37593
                     /gene="ilvN"
                     /locus_tag="RH058_001163"
     CDS             37303..37593
                     /gene="ilvN"
                     /locus_tag="RH058_001163"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV3136782.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37668..38258
                     /gene="uhpA"
                     /locus_tag="RH058_001164"
     CDS             37668..38258
                     /gene="uhpA"
                     /locus_tag="RH058_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV3136783.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38258..39760
                     /gene="uhpB"
                     /locus_tag="RH058_001165"
     CDS             38258..39760
                     /gene="uhpB"
                     /locus_tag="RH058_001165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV3136784.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39770..41089
                     /gene="uhpC"
                     /locus_tag="RH058_001166"
     CDS             39770..41089
                     /gene="uhpC"
                     /locus_tag="RH058_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV3136785.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            41227..42618
                     /gene="uhpT"
                     /locus_tag="RH058_001167"
     CDS             41227..42618
                     /gene="uhpT"
                     /locus_tag="RH058_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV3136786.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42663..44429)
                     /gene="adeD"
                     /locus_tag="RH058_001168"
     CDS             complement(42663..44429)
                     /gene="adeD"
                     /locus_tag="RH058_001168"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV3136787.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
BASE COUNT        10215 a        11440 c        12071 g        10709 t
ORIGIN      
        1 ataacaggag tgctaacaat gaagcgaagc cgcatctgac agtcagaata cggcttcgta
       61 agcgcggtta cttaccaata cagaagctgg agaaaatccg ccccagcagg tcatcagaag
      121 taaattcccc ggtgatttcg cttaagttct gctgtgccag gcgcaactct tccgccagca
      181 gttcgcccgc ccaggcacct aacagttgcg ctttaccttg ctgcaaatgc tctgccgcct
      241 gttccagcgc ctgtaggtgg cgacggcgcg ccaggaagcc gccttccatg ttggtgtcaa
      301 agcccatgct ctgtttgaga tggttacgca gcacatccac accttcacca gtccttgccg
      361 agagacgaat taacgcgtga ccgttcactt cactcattcc cagcgtttcg ccggtgatat
      421 cggctttatt gcgcaccacg gtgatcggca gtttcgctgg cagacgggcg ataaattccg
      481 gccagatctc tgccggatca acggcgtctg tcgtggtgcc atcgaccata aacagcacgc
      541 ggtcggcctg ttcaatttcc tgccacgcgc gctcgatacc aatacgttcc acttcgtcac
      601 tggcttcacg tagcccagcg gtatcgatga tatgcagcgg cattccgtca atgtggatat
      661 gctcacgcag cacgtcacgt gtggttccgg cgatatcagt tacgattgct gcttcacggc
      721 ccgccagcgc gtttaacagg ctcgatttac cggcgttagg acgtccggca atcaccacct
      781 tcatcccttc gcgcaacaaa ctaccctgac gtgcttcagc acgcactgca tcgagttcgg
      841 caatcacgtc attaagctgg gcttcaattt tgccgtcgga gaggaaatcg atctcttcat
      901 cagggaaatc aattgccgct tcgacgtaaa tgcgcaagtg ggtgagggct tccaccagat
      961 ggttgacccg tgcggagaat gcgccttgca gcgagttaag tgccgaacgg gccgcctgtt
     1021 ccgaactggc gtcgataaga tcggcaatcg cctcggcctg ggctaagtcg agtttatcgt
     1081 taagaaacgc gcgttcggaa aactcaccag ggcgagcaat ccgcaggccg ggaatggtca
     1141 gaatgcgttt taacagcagg tcgaggatca ccggaccgcc atgaccttgc agttccagca
     1201 catcttcgcc ggtgaacgag ttcgggccag ggaaccatag cgcaatcccc tgatcgagca
     1261 cgctgccgtc ggcgtcttta aacggaagat aatcggcgta gcgcggctta ggtagtttac
     1321 ccagcacggt ttcggcaact tcacgggctt tgaagccgga gatgcgcagg atgccaacgc
     1381 cgccacgtcc cggaggcgtg gcctgggcta cgatagtgtc attatcgctc atgatgttcc
     1441 tgttgctttg tgtggcggat gcgcggtgct tatccgccct acgaaaagaa aaaaggcggt
     1501 caactgaccg cccttatttt agcgaaaact caccgaatca ggattttttc ttctcgcggc
     1561 tatgcaggcc acgtttttcc agaccacggt aaatcagctg ctgctgaata atggttacca
     1621 ggttgctgac gatatagtac agcaccagac ctgacgggaa ccacaggaag aacacggtga
     1681 agatgaccgg cataaaggtc atgatcttct gctgcatcgg gtcggtcact gtggtcggcg
     1741 acatcttctg aatgaagaac atcgttacgc ccatcaggat cggcaagatg tagtacgggt
     1801 cctgtgccga caggtcgtgg atccacagtg caaacggtgc ctgacgcagt tcaacggaac
     1861 ccatcagcat gtagtacaac gccaggaaga ttggcatctg gatcagcagc gggaagcagc
     1921 cgcccagcgg gttaaccttc tcagctttgt acagcgccat catttcctgg ctgatacgct
     1981 gtttgtcatc gcccagacgc tcacgcattg cctgaatctt cggctgcagc atacgcatct
     2041 tcgccatgga ggtgtactgc gctttggtca gcgggtacat gatgccacga acgataaagg
     2101 tgatgatgat aatggagaag ccccagttac ccacaaagct atggatccat ttcagcagtt
     2161 tgaacagcgg ctgagagatg aaccacaacc aaccgtaatc aacggtcaga tccaggtgcg
     2221 gagcaacagc tgccattttg tcctggattt ccgggccaac ccacagggtg ctgttcatcg
     2281 cgccagtctg accaggctga accagtaccg gctgagattt atagccgata gcggcgatgc
     2341 cgttacccag attagcggta tagaagttgt tggtaccgtc gttatgcggg atccacgccg
     2401 tcgcgaaata ctgttgcagc atcgccaccc aaccaccttt cgaagagatg ttcaggtttt
     2461 cgttatcggc aatggtatcg aacttgtatt tctcgtactt ctcgtcaggc gtggagtacg
     2521 ccgcgccgcg gaaggtgtgc agtgcgaagt tgctgcttcc ggtatcgaga tgcggtggca
     2581 gagtgatgga ttgcttcaac tgaccaaagg tggagatttc cagcggtttc tcgccagcgt
     2641 tctgcacgtt gtagttgacg ttgacagcgt aatcaccacg tttcaggaca aacgttttgg
     2701 taaacgtgtt gcctgccgcg tcggtatacg tcatcggcac ctgcagttcg ttttgacctt
     2761 cagccagcac ataagcgtct ttttcaacgt tatacagcgg acgcgggccg ttagccgggt
     2821 tatccgggcc atcacgaccg gtcagaccgc tctgtgcctg ataaataaac tgcggtgaag
     2881 tttccagcag ctggaacggc tgggtagagt tcagctcttt cgggtaagca ggcagcagag
     2941 cttgctcaac atcaccacca cgggtgttga tggtcagatc aagcacgtcg gtcttaaccg
     3001 agatcagttt cccctggcca ctggccggta cgccctggtc ggcggcgcta cccgctgcgg
     3061 tggtcgttgt ctgcgtggtc tgttgggcct gaggttgcgg gtttttatcc tgctcccagg
     3121 cttgccagat catgaaagac acgaacagca aagcgatgac taaaagattg cgttgcgaat
     3181 ccatcgttag tgttctctgg tatcaaatgg tccgggcggg acgggatcgt caccaccagg
     3241 gtgtaaaggg tggcatttta atacgcgttt caccgtcaac caactgcctt ttatcactcc
     3301 aaacctgcgc aatgcctcaa ttccgtagct tgaacaggtt ggagtgaaac gacaatgcgg
     3361 cccgagtagc ggactaatca ggcgttgata gacccgaatg agggctatca ggacccgcga
     3421 gccaggcgac agtggcggcg ccataatttt tccaacgctt ccgagagagc acggttatcg
     3481 aggtcggcaa cccctttttt cgccaccacc acgaaatcca tagccgggag ttcatgttgg
     3541 cgcagacgga agctttcacg cgtcagacgt ttaatccgat tgcgttcatg ggcgcgtcga
     3601 acgtttttct tggcgactgt aagaccgata cggggatgcc ccagcgaatt caggcggccg
     3661 agaatggtaa tttgcggcgt gccagcccgt tgtggctgct ggaagacgaa tgtgaattga
     3721 ctgggagtta acaagcgtaa ctccctggga aatgcgagct taaccactca ggggttagct
     3781 ttattactta gaaacggtca gacgagcgcg gcctttagca cgacgacgtg ccagaacctg
     3841 acgaccattt ttagtagcca tacgagcacg gaagccgtga gaacggttgc gcttcagtac
     3901 agacggttga aaagtgcgtt tcatggcgat ttctacctaa acttgaataa attcaatggc
     3961 tttattggat atccgccgaa aaatgaaacg atggacaccg aagccatggg tgattaaaga
     4021 ggccggattg taataattgt acactccgga gtcaattctc tttccttatt taccgcgctt
     4081 ttccgcacct tttcgcaggg aaaatgtacg acctcacacc agtggaaacc agcatggcgc
     4141 gccgggtgga ggattatacg ggctgatggg taaagcgcaa ggatcgtcct ggatctttat
     4201 tagatcgatt aagccaattt ttgtctatgg tcattaaatt ttccaatatg cggcgtaaat
     4261 cgtgcccgcc tcgcggcagg atcgtttaca cttagcgagt tctggaaagt cctgtggata
     4321 aatcgggaaa atctgtgaga aacagaagat ctcttgcgca gtttaggcta tgatccgcgg
     4381 tcccgatcgt tttgcaggat cttgatcggg catataaccg cagacagcgg ttcgtgcgtc
     4441 accctcaagc agggtctttt cgacgtacgt caacaatcat gaatgtttca gccttagtca
     4501 ttatcgactt ttgttcgagt ggagtccgcc gtgtcacttt cgctttggca gcagtgtctt
     4561 gcccgattgc aggatgagtt accagccaca gaattcagta tgtggatacg cccattgcag
     4621 gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt cctcgattgg
     4681 gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg cggagcggat
     4741 gccccacagc tgcgttttga agtcggcacc aaaccggtga cgcaaacgcc acaagcggca
     4801 gtgacgagca acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg
     4861 ccttctacgc gctcaggttg ggataacgtc ccggccccgg cagaaccgac ctatcgttct
     4921 aacgtaaacg tcaaacacac gtttgataac ttcgttgaag gtaaatctaa ccaactggcg
     4981 cgcgcggcgg ctcgccaggt ggcggataac cctggcggtg cctataaccc gttgttcctt
     5041 tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa cggcattatg
     5101 gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt tcaggacatg
     5161 gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg ttccgtagat
     5221 gcactgctga tcgacgatat tcagtttttt gctaataaag aacgatctca ggaagagttt
     5281 ttccatacct tcaacgccct gctggaaggt aatcaacaga tcattctcac ctcggatcgc
     5341 tatccgaaag agatcaacgg cgttgaggat cgtttgaaat cccgcttcgg ttggggactg
     5401 actgtggcga tcgaaccgcc agagctggaa acccgtgtgg cgatcctgat gaaaaaggcc
     5461 gacgaaaacg acattcgttt gccgggcgaa gtggcgttct ttatcgccaa gcgtctacga
     5521 tctaacgtac gtgagctgga aggggcgctg aaccgcgtca ttgccaatgc caactttacc
     5581 ggacgggcga tcaccatcga cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag
     5641 gaaaaactgg tcaccatcga caatattcag aagacggtgg cggagtacta caagatcaaa
     5701 gtcgcggatc tcctttccaa gcgtcgatcc cgctcggtgg cgcgtccgcg ccagatggcg
     5761 atggcgctgg cgaaagagct gactaaccac agtctgccgg agattggcga tgcgtttggt
     5821 ggtcgtgacc acacgacggt gcttcatgcc tgccgtaaga tcgagcagtt gcgtgaagag
     5881 agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc gtaaacctat
     5941 gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga gcggtccgtt
     6001 aggtggtcgt cctacgctac cgattctcgg taatctgctg ttacaggttg ctgacggtac
     6061 gttgtcgctg accggtactg atctcgagat ggaaatggtg gcacgtgttg cgctggttca
     6121 gcaacacgag ccaggagcga cgaccgttcc ggcgcgcaaa ttctttgata tctgccgtgg
     6181 tctgcctgaa ggcgcggaaa ttgccgtgca gctggaaggt gaacggatgc tggtacgctc
     6241 cgggcgtagc cgtttttcgc tgtctaccct gccagcggcg gatttcccga acctcgatga
     6301 ctggcagagt gaagtcgaat ttaccctgcc gcaggcaacg atgaagcgtc tgattgaagc
     6361 gacccagttt tctatggcgc atcaggacgt tcgctattac ttaaatggta tgctgtttga
     6421 aaccgaaggt gaagaactgc gcaccgtggc aaccgacggc caccgtctgg cggtctgttc
     6481 aatgccaatt ggtcaatctt tgccaagcca ttcggtgatc gtaccgcgta aaggcgtgat
     6541 tgaactgatg cgtatgctcg acggcggcga caatccgctg cgcgtacaga ttggcagcaa
     6601 caacattcgc gcccacgttg gcgactttat cttcacctcc aaactggtgg atggtcgctt
     6661 cccggattat cgccgcgttc tgccgaagaa cccggacaaa catctggaag ctggctgcga
     6721 tctgctcaag caggcgtttg ctcgcgcggc gattctctct aacgagaaat tccgcggcgt
     6781 acgtctttat gtcagcgaaa accagctgaa aatcaccgcc aacaacccgg aacaggaaga
     6841 agcggaagag atcctcgacg ttacctatag cggtgcggag atggaaatcg gcttcaacgt
     6901 cagctatgtg ctggatgttc tgaacgcgct gaaatgcgaa aacgtccgca tgatgctgac
     6961 cgattcggtt tccagcgtgc agattgaaga tgccgcatca cagtcggctg cctatgttgt
     7021 catgccaatg agactgtaat gtccctcacc cgcttgttga tccgcgattt ccgcaacatt
     7081 gaaaccgcgg atctcgcttt atctcccggc tttaactttc tggtaggtgc caacggcagt
     7141 ggcaaaacca gcgtgctgga agccatctat acgctcggcc atggtcgggc gtttcgcagt
     7201 ttgcagattg gtcgcgtcat tcgccatgag caggaggcgt ttgttctcca cgggcgatta
     7261 cagggcgaag agcgcgagac agcgattggc ttaaccaaag acaaacaggg cgacagcaaa
     7321 gtccgcatcg acggtacaga cgggcataag gtcgcggaac tggcgcacct gatgccaatg
     7381 cagttgataa cgccagaagg gtttacttta ctcaacggcg gccccaaata cagaagagca
     7441 ttcctcgact ggggatgctt tcacaacgaa cccggatttt tcaccgcctg gagcaatctc
     7501 aagcgattgc tcaagcagcg caatgcggcg ctgcgccagg tgacacgtta cgaacagcta
     7561 cgcccgtggg ataaagaact gatcccgctg gcggagcaaa tcagcacctg gcgcgcggag
     7621 tatagcgccg gtatcgcggc tgatatggcc gatacttgta agcaatttct ccctgagttt
     7681 tctctgactt tctctttcca gcgcggctgg gagaaagaga cagaatatgc tgaggtgctg
     7741 gaacgtaatt ttgaacgcga tcgccagcta acctacaccg cgcatggccc gcataaagcg
     7801 gacttacgca ttcgcgccga cggtgcgccg gtggaagata ccttatcgcg tgggcagctt
     7861 aagctgttga tgtgcgcctt acgtctggcg caaggagagt tcctcacccg tgaaagcggg
     7921 cggcggtgtc tctacctgat agatgatttt gcctctgagc ttgatgatga gcgtcgcggg
     7981 ctgcttgcca gccgcttaaa agcgacgcaa tcacaggtct ttgtcagcgc gatcagtgct
     8041 gaacacgtta tagacatgtc ggacgaaaat tcgaagatgt ttaccgtgga aaagggtaaa
     8101 ataacggatt aacccaagta taaatgagcg agaaacgttg atgtcgaatt cttatgactc
     8161 ctccagtatc aaagtcctga aagggctgga tgcggtgcgt aagcgcccgg gtatgtatat
     8221 cggcgacacg gatgacggca ccggtctgca ccatatggta ttcgaggtgg tagataacgc
     8281 tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc gtcaccattc acgccgataa
     8341 ctctgtctct gtacaggatg acgggcgcgg cattccgacc ggtattcacc cggaagaggg
     8401 cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca ggcggtaaat ttgacgataa
     8461 ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt tcggtagtaa acgccctgtc
     8521 gcaaaaactg gagctggtta tccagcgcga gggtaaaatt caccgtcaga tctacgaaca
     8581 cggtgtaccg caggctccgc tggcggttac cggcgagact gaaaaaaccg gcaccatggt
     8641 gcgtttctgg cccagcctcg aaaccttcac caatgtgacc gagttcgaat atgaaattct
     8701 ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc gtttccattc gtctgcgcga
     8761 caagcgtgac ggcaaagaag accacttcca ctatgaaggc ggcatcaagg cgttcgttga
     8821 atatctgaac aagaacaaaa cgccgatcca tccgaatatc ttctacttct ccaccgaaaa
     8881 agacggtatt ggcgtcgaag tagcgttgca gtggaacgat ggcttccagg aaaacatcta
     8941 ctgctttacc aacaacattc cgcagcgtga cggcggtact cacctggcag gcttccgtgc
     9001 ggcgatgacc cgtaccctga acgcctacat ggacaaagaa ggctacagca aaaaagccaa
     9061 agtcagcgcc accggtgacg atgcgcgtga aggcctgatt gcggtcgttt ccgtaaaagt
     9121 gccggacccg aaattctcct cccagaccaa agacaaactg gtttcttctg aggtgaaatc
     9181 ggcggtagaa cagcagatga acgaactgct ggcagaatac ctgctggaaa acccaaccga
     9241 cgcgaaaatc gtggttggca aaattatcga tgctgcccgt gcccgtgaag cggcgcgtcg
     9301 cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta gcgggcctgc cgggcaaact
     9361 ggcagactgc caggaacgcg atccggcact ttccgaactg tacctggtgg aaggggactc
     9421 cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac caggcgattc tgccgctgaa
     9481 gggtaaaatc ctcaacgtcg agaaagcgcg cttcgataag atgctctctt cccaggaagt
     9541 ggcgacgctt atcaccgcgc ttggttgtgg tatcggtcgt gacgagtaca acccggacaa
     9601 actgcgttat cacagcatca tcatcatgac cgatgcggac gtcgacggct cgcacattcg
     9661 tacgctgctg ttgaccttct tctatcgtca gatgccggaa atcgttgaac gtggtcacgt
     9721 ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc aagcaggaac agtacattaa
     9781 agacgacgaa gcgatggatc agtaccagat ctctatcgcg ctggacggcg caacgctgca
     9841 caccaacgcc agcgcaccgg cattggctgg cgaagcgtta gagaaactgg tgtctgagta
     9901 caacgcgacg cagaaaatga tcaaccgcat ggagcgtcgt tatccgaaag caatgctgaa
     9961 agagcttatc tatcagccga cgctgacgga agccgacctc tctgatgagc agaccgttac
    10021 ccgctgggtg aacgcgctgg tcagcgaact gaacgacaaa gaacagcacg gcagccagtg
    10081 gaagtttgat gtccacacca atgccgaaca aaacctgttt gaaccgattg ttcgcgtgcg
    10141 tacccacggt gtggatactg actatccgct ggatcacgag tttatcaccg gtggcgaata
    10201 tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg ctggaagaag atgcgtttat
    10261 cgaacgtggc gagcgtcgtc agccggtagc cagcttcgag caggcgctgg actggctggt
    10321 gaaagagtcc cgtcgcggcc tctccatcca gcgttataaa ggtctgggcg agatgaaccc
    10381 ggaacagctg tgggaaacca ccatggaccc ggaaagccgt cgtatgctgc gcgttaccgt
    10441 taaagatgcg attgctgccg accagttgtt caccacgctg atgggcgacg ccgttgaacc
    10501 gcgccgtgcg tttattgaag agaacgccct gaaagcggcg aatatcgata tttaatggcg
    10561 ctaaccatgc gagcgtgcct gatgcgctac gcttatcagg cctacgaaaa tcttgcaatg
    10621 tattgaattt tcatgatttt gtaggccgga taaggcgttc acgccgcatc cggcaagaag
    10681 ctataagaaa agggcggaga tcatctccgc cctttttatt tctgcaatcc gcaattatca
    10741 tcccgcaaat ctatactttt tgggtcaaca cgtaacgctt tacaggtgat tatcatgggg
    10801 ctttttgatg aagttgtcgg tgcctttctg aaaggcgatg cggggaaata tcaggctatt
    10861 ttaagttggg ttgaggagca gggcggcatt caggtgctgc tggaaaaact gcaaagtggc
    10921 ggcttagggg ccattctctc aacctggctg agtaatcaac agggcaatca atcggttagt
    10981 ggcgagcaac tggaatcggc gctcggcaca aatgcggtgt ccgatcttgg acaaaaactt
    11041 ggcgtggata ccagtacagc ttccagttta ctggcagaac aattgccgaa gattattgat
    11101 gcgctctcac cgcaaggtga agtgtctcca caagccaata acgatctgct ttccgcaggc
    11161 atggaactgc tgaaagggaa actcttccgc taagcaaatg gggagcatgc aacccgcgtt
    11221 gcatccccct tttccctcgg gtgttgtact gatttttgag cggaatcgcg ttagcatggg
    11281 tcaggaacta atctacctgg ggaactcatg gctatcaaac tcattgctat cgatatggat
    11341 ggcacccttc tgctacccga tcacaccatt tcacccgccg ttaaaaatgc gattgccgca
    11401 gctcgcgccc gtggcgtgaa tgtcgtgcta acgacgggtc gcccttatgc aggtgttcac
    11461 aattacctga aagagctgca tatggaactg ccgggcgact actgcattac ttataacggc
    11521 gcgctggtac agaaggccgc tgatggtagc accgtggcgc aaactgctct cagctatgac
    11581 gactaccgtt tcctggaaaa actctctcgc gaagtcggtt ctcatttcca cgccctggac
    11641 cgcaccacgc tgtacaccgc caaccgtgat atcagctact acacggtgca tgaatccttc
    11701 gttgccacaa ttccgctggt gttctgcgaa gcggagaaaa tggaccccaa tacccagttc
    11761 ctgaaagtga tgatgattga tgaacccgcc atcctcgacc aggctatcgc gcgtattccg
    11821 caggaagtga aagagaaata taccgtgctg aaaagtgcgc cgtacttcct cgaaatcctc
    11881 gataaacgcg ttaacaaagg tacgggggtg aaatcactgg ccgacgtgtt aggtattaaa
    11941 ccggaagaaa tcatggcgat tggcgatcag gaaaacgata tcgcaatgat tgaatatgca
    12001 ggcgtcggtg tggcgatgga taacgctatt ccttcggtga aagaagtggc gaactttgtc
    12061 accaaatcca accttgaaga tggcgtggcg tttgctattg agaagtatgt gctgaattaa
    12121 tcagtggacg ggcaaacagc ataaacgcgt ttgcccgctt actgattatt ggtttactgc
    12181 atatacaacc agaaattgca gcggtgcagt aatgatgtcc agcgtaacag tcactggcag
    12241 taatgctgcg gaaacaccat ccggaatgcc tctgggtgaa tagtattcat aaaatcgcac
    12301 tttaaacgga tgatagaacg ccatcacctt tgagtaatcc atgtttttat ttttctcatg
    12361 gattgtgcct tttaggtttt ccagtgacct ggtacagtgt tgaacaccac aggcaaaacc
    12421 ataatgcgtt gccagtgcct tttcttcttc gttattccag tagtagtaaa gcgatattgt
    12481 gccggtaaat ttctttttcc ccgcatttaa aacaaatctt gcgtcatcgg caacctgaat
    12541 attgtgccgg ttaagtgctg gatcattgag catcttaacg atgtcatcgg caccatcagt
    12601 gataaggtaa tccagcgatt cccctacaaa aacatagcct tttgttccat tactatcttt
    12661 tgcctgcgat agcccagtga tggtgtctga ctgcgattga tactttcctg aactaccctt
    12721 tgcggtatgg gaaaccagag catacgagat acagcctgaa agcatcagcg ccagtgggaa
    12781 taaaccggca gccttaaaaa atcccttaaa attcaacttc atgattaatc ctttaaacga
    12841 gtagctaagc cgttgaaagc atactcttct ttccctcctg tttagcgcct gatatccctt
    12901 ttcagatttc tgcccgacgc atgtcatttt tttatgcatt gttctttttg tgatctaaat
    12961 tgtagtacaa caatataagt ttgtactaca ttacgcacac ggcaaacgcg aacgtcatca
    13021 cgctggtact acaaagttgc cgcgttatgc atcgatcggg gtaaagtaga gaagaacata
    13081 cagagcacaa ggactctcca tgactctcaa taaaaccgat cgcattgtca ttacgctggg
    13141 taaacagatc gttcacggca aatacgtacc tggctcgcca cttccggctg aggcggagct
    13201 ctgtgaagag tttgcaacct cgcgcaacat catccgtgag gtgttccgtt cgctgatggc
    13261 gaagcggctg attgaaatga aacgttatcg cggggcgttt gtggcaccgc gtaaccagtg
    13321 gaattacctc gacactgacg tactgcaatg ggtgttggaa aacgactacg acccacggct
    13381 tatcagtgcc atgagcgaag tgcgaaatct ggtggaaccg gcgattgccc gttgggcagc
    13441 agagcgcgcg acctccagcg atctggcgca gattgaatcg gcgctgaacg agatgattgc
    13501 caacaatcag gaccgcgaag cgtttaacga agcggatatt cgctaccacg aggcggtgct
    13561 gcagtcggtg cataacccgg tgttacagca acttagcatt gcgatcagtt cgctgcagcg
    13621 ggcggttttt gaacgaacct ggatgggcga tgaggccaac atgccgcaaa cgctccagga
    13681 acataaggcg ctgttcgatg cgatacggca tcaggacggc gatgcggcag agcaggcggc
    13741 gcttaccatg atcgccagct cgacacgaag gttaaaggaa atcacatgac agctcgctac
    13801 atcgcaattg actggggatc gaccaatctt cgcgcctggc tttatcaggg cgaccactgc
    13861 ctggagagca ggcaatcaga agcaggcgtc acgcgcctga acggaaaatc tccggctgcg
    13921 gtgttagcag aagtcacgac cgactggcgt gaagagaata cgccagtggt aatggcagga
    13981 atggtcggca gcaatgtcgg ctggaaagtt gctccgtatt tatctgttcc tgcccgtttt
    14041 tcgtctattg gcgaacaatt aacgtctgtt ggcgacaata tctggattat tcccggatta
    14101 tgcgtctctc atgacgataa ccacaatgtg atgcgcggcg aagaaacaca attgatcggc
    14161 gcgcgaactc tggctccttc ctctctttat gttatgcccg gtacgcattg caaatgggtg
    14221 caggccgata gccagcaaat caacgatttt cgcaccgtga tgaccggtga actacatcat
    14281 ttactgttaa atcactcatt gattggcgca ggtttgccgc cgcaggaaaa ctctgccgat
    14341 gccttcgcgg ctggccttga gcgcggcctt aatgcgcccg ccatattgcc gcagcttttt
    14401 gaagttcgcg cctcgcatgt gctgggaaca cttccccgcg aacaggtcag cgaatttctc
    14461 tctggtttgt tgattggcgc agaggttgcc agtatgcgcg actatgtgac ccatcaacac
    14521 gccatcaccc ttgtcgccgg aacatcgctg accgcgcgct accagcaagc ctttcaggcg
    14581 atgggttgcg acgtgacggc ggtggcgggc gacacggcat ttcaggctgg tataaggagc
    14641 atcgctcatg cagtggcaaa ctaaacttcc gctgatcgcc attttgcgcg gcattaagcc
    14701 cgacgaggcg ctggcgcatg ttggcgcggt gattgacgcc gggttcgacg cggttgaaat
    14761 cccgctgaat tccccacaat gggagcaaag cattcccgcc atcgttgatg cgtatggcga
    14821 caaggcgttg attggcgcag gtacggtact gaaacctgaa caggtcgatg cgctcgccag
    14881 gatgggctgt cagctcatcg ttacgcccaa tatccatagt gaagtgatcc gccgtgcggt
    14941 gggctacggc atgaccgtct gccccggctg cgcgacggcg accgaagcct ttaccgcgct
    15001 cgaagcgggc gcgcaggcgc tgaaaatatt tccgtcatcg gcttttggtc cgcaatacat
    15061 caaagcgtta aaagcggtat tgccatcgga catcgcagtc tttgccgttg gcggcgtgac
    15121 gccagaaaac ctggcgcagt ggatagacgc aggttgtgct ggggcgggct taggcagcga
    15181 tctctatcgc gccgggcagt ccgtagaacg caccgcgcag caggcagcag catttgttaa
    15241 ggcgtatcga gaggcagtgc aatgaaaatc accaaaatta ccacgtatcg tttacctccc
    15301 cgctggatgt tcctgaaaat tgaaaccgat gaaggcgtgg tcggttgggg cgagcccgtg
    15361 attgaaggcc gcgcccgtac ggtggaagcg gcagttcacg agctgggtga ctatttgatt
    15421 ggtcaggatc cttcgcgcat caatgactta tggcaagtga tgtatcgcgc cggattttat
    15481 cgtggcggtc caatcctgat gagcgccatt gccgggatcg accaggcgtt atgggatatc
    15541 aaaggcaaag tgctgaatgc gccggtctgg caactgatgg gcggcctggt tcgcgacaaa
    15601 attaaagcct acagttgggt cggcggcgat cgtccggcgg atgttatcga cggcattaaa
    15661 accctgcgcg aaatcggctt cgataccttc aaactgaacg gttgtgaaga actggggcta
    15721 attgataact cccgcgcggt agatgcggca gtcaacaccg tggcacaaat tcgtgaagct
    15781 tttggcaatc agattgagtt tggtcttgat ttccacggtc gcgtcagcgc gccaatggcg
    15841 aaagtgctga ttaaagaact ggagccgtat cgcccgctgt ttattgaaga gccggtgctg
    15901 gcggaacaag ccgaatacta cccgaaactg gcggcacaaa cgcatattcc actggcggcg
    15961 ggtgagcgca tgttctcacg tttcgatttt aaacgcgtgc tggaggcagg cggtatttcg
    16021 attctgcaac cggatctctc ccatgcaggc ggtattaccg aatgctacaa aattgctgga
    16081 atggcagaag cctatgatgt gacccttgcg ccgcactgtc cgctcggacc gattgcactg
    16141 gcggcttgcc tgcatatcga ctttgtttcc tataacgcgg tacttcagga acaaagtatg
    16201 ggcattcatt acaacaaagg cgcggagtta ctcgactttg tgaaaaacaa agaagacttc
    16261 agtatggttg gcggcttctt taaaccgtta acgaaaccgg gcttaggtgt ggaaatcgac
    16321 gaagctaaag ttattgagtt cagtaaaaat gccccggact ggcgtaatcc gctctggcgt
    16381 catgaagata acagcgtagc agagtggtaa ttcctgccac gtaagcccct catcgggcac
    16441 taaaacagca atacaaaaat ataaccctct gtaaattaca gggcatggtg agcggcttcg
    16501 ctatgcccaa aatctggaga aagctcgcta tggatattcc cgttaatgca gcaaagccgg
    16561 ggcgtcggcg ttatctgacg ctggtgatga tctttattac ggtggtcatt tgttatgttg
    16621 accgcgctaa cctggccgtg gcttccgccc atattcagga agagttcggc attacgaaag
    16681 cggaaatggg ctatgtattt tcggccttcg cctggcttta tacgctatgc cagatccccg
    16741 gcggttggtt tttagatcgc gtaggttctc gcgtgactta ttttattgcg atatttggtt
    16801 ggtcagtggc gactttattc cagggctttg ccacgggatt aatgtcatta attggtctgc
    16861 gtgcgataac cggtattttc gaagcgcccg ctttcccgac caataaccgg atggtgacca
    16921 gctggttccc ggaacatgaa cgcgcttccg ccgttggttt ttatacgtct ggtcagtttg
    16981 tcggtctggc gtttttgact ccgctgctga tctggattca ggagatgttg agctggcact
    17041 gggtgttcat tgtcactggt ggtatcggca ttatctggtc gctgatttgg tttaaggttt
    17101 atcagccgcc gcgcctgacc aaaggtatca gcaaagctga actggattac attcgtgatg
    17161 gcggcggtct ggtggatggt gatgcgccgg tgaagaaaga ggcgcgtcag ccgttaacag
    17221 ccaaagactg gaaactggtg ttccatcgta aactgatcgg cgtttatctt gggcaatttg
    17281 cggtggcttc tacactgtgg tttttcttaa cctggttccc gaactattta acccaggaaa
    17341 aaggaatcac ggcgctgaaa gcaggcttta tgaccacggt gccattcctc gcggcgtttg
    17401 tcggcgtcct gctctctggc tgggtcgcgg atctgctggt acgtaagggc ttttcactgg
    17461 gctttgcgcg taaaacgccg attatctgcg gcttgctgat ctccacctgc attatgggcg
    17521 ctaactacac caacgatccg atgatgatta tgtgcctgat ggcgctggca ttcttcggca
    17581 acggttttgc ttcgattacc tggtcgctgg tctcttctct ggcaccgatg cgcctgattg
    17641 gtttaaccgg cggcgtgttt aacttcgccg gtggtctggg cggcatcacc gttccgctgg
    17701 tggtggggta cctggcgcag ggttacggtt tcgcacctgc actggtttat atctccgccg
    17761 tcgcgttgat tggcgcgctc tcttacatcc tgctggtggg cgatgtgaag cgcgttggct
    17821 aatctttcaa ctgtggaata tgcgccatac cactgcgcat aatccacttt cttaaagccg
    17881 gtgcggtcag gcatcggctt tttactatct tgccgaagag ttttaaacgc agtttgcggg
    17941 tggcacgcca gtaagcggta ttgagcttct ctggctgctt cagtaacacc gcacgcagaa
    18001 tgtctgcgct atccagcgca tagctaatcc cctccagcga actggcgctg ataaatcctg
    18061 ccgcttcgcc aatcagaaag gcgttgtcct taccgcagac aaaatcctgc cagcgtgagg
    18121 gaaacagcac cgtgcatttt tcgcttttca ccgccttacc aaactggaac tgaaaggcgc
    18181 tcattttctc tttcagcgtc gtgaaacgcg tctgaccgtc tttcattgga taagcaccgc
    18241 caaagataaa ataaccgtct ttgctgatac tccatgaata acagttagtt atcgaattat
    18301 caaagatgca ggagtagaac ggcaccggat gtttctccgc gaaccactgc tggatagcga
    18361 catatttacg gatttgatga tccgggtaga gatgtcgccg caccatcgag tttgcgccat
    18421 cggcaccgac cagatagcgg gcggtaatat gttgctccca gccgtctgca cgaaaaatga
    18481 catgccattt atcatcctca cgccagatgt ttcggcacag gctatcgtga taaacctcaa
    18541 cgctggtggg gatcagcgat ttcatccaca agtcgaaagc atggcgatta atattgatat
    18601 agcttcgctg gtagttacgc gtgagcgatg cggcgacgtc gacagttttg acgctgaaaa
    18661 tctgtggatt ggcgatcaca tcgacaggaa gcgtcagtcc atcgcgaata aaagatcgct
    18721 gcgcgtccgg tgccagcaga ccgccgcaag gtttgctgaa accttcagta ccacactggt
    18781 gctttttatc cagcgcgatc acctgcattt tgcctgctaa ttttcgcgcc aacgccgatc
    18841 ctgccgggcc gaggccaata atcgccacgt cgaaatgttc catcttttca ctcacattca
    18901 tcacgatggc aaaagactat tgtctgaaag tgaagtcctt gtgaagtatg atggtgggac
    18961 ccgttttgta tcaggaccgc gccatgaaac agataacctt tgctccccgt aatcacctgc
    19021 tcaccaatac caatacctgg acgcccgaca gccagtggct ggtatttgac gtgcgtcctt
    19081 ctggggcgtc gtttaccggc gagaccattg agcgtgtgaa tatccatacc ggcgaggtcg
    19141 aggttatcta tcgcgcgtca cagggcgcac acgtcggcgt ggtgaccgtt catccgaagt
    19201 cagagaaata tgttttcatc catggcccgg aaaatcctga tgaaacatgg cattacgatt
    19261 tccatcatcg acgcggagtc attgctgaag gcggcaaggt gagcaatctc gatgcaatgg
    19321 atattaccgc gccgtatacg ccaggagcgc tgcgcggcgg cagccatgtg catgtcttta
    19381 gcccgaacgg tgaaagggtg agctttacct ataacgacca tgtaatgcat gaactcgatc
    19441 cggcgctgga tttgcgaaac gtcggtgttg ctgcgccgtt tggcccggtc aacgtacaaa
    19501 agcagcatcc gcgtgaatac agcggtagcc actggtgcgt gctggtgagt aaaaccacgc
    19561 ccacgccgca gcctggcagc gatgaaatca atcgtgctta tgaagaagga tgggtaggaa
    19621 atcacgcgct ggcgtttatt ggcgacacac tttcgccaaa gggcgagaaa gtgccggagc
    19681 tgtttatcgt tgagttaccg caagatgaag ctggctggaa agcggcaggt gatgcgccgt
    19741 taagtggaac ggaaaccacc ctgcccgcgc caccgcgcgg cgtcgtgcag cgacgtttaa
    19801 cctttaccca ccatcgggct tatccggggt tagtcaacgt cccgcgccac tgggtgcgct
    19861 gtaatccgca gggtacgcaa atcgcgtttt taatgcgtga tgataacggc attgtgcaac
    19921 tgtggcttat ctcgccacag ggcggcgagc cgcgccagtt aacccataac aaaacggata
    19981 ttcagtctgc atttaactgg catccgtcag gagaatggtt gggctttgtg ctggataatc
    20041 gaattgcttg tgcccatgcg caaagcggcg aggttgagta tttaaccgaa aaccacgcca
    20101 atccaccttc tgcggacgcc gtggtcttct cgccggatgg tcagtggctg gcgtggatgg
    20161 aaggcggcca gctgtggatc accgaaactg atcgctaaat cagttatcag gcattctggc
    20221 cggtggaata accgcattgg tggcttgcgc gttggcttcg cttttttcaa cgcgtgagcg
    20281 caccgaactg tctttgcgga acacatccca cggcagtaaa agcgtgtcca tcacagcggt
    20341 aaatggcata tcgagaatcg ccagcgactt agtgccccag ttggtttcat cgtcgccaat
    20401 catggttgcg ctggcgcggg tgccgggata cgttccttct ttccctccgg tatgggacat
    20461 cacgctggag catcctccga gtaatgtcat tccgctgcat atcatgaacg ctaacaacac
    20521 atttcttatc ataataaatt catctgttga tcgtgggtgt tggcctgatg agttatagcg
    20581 atcccttgct gaaaataaca tcatcattac gtcgcactgt ggcggctatc gcactttaac
    20641 gtttcgtgct gccccctcag tctatgcaat agaccataaa ctgcaaaaaa aagtccgctg
    20701 ataaggcttg aaaagttcat ttccagaccc atttttacat cgtagccgat gaggacgcgc
    20761 ctgatgggtg ttctggctac ctgacctgtc cattgtggaa ggtcttacat tctcgctgat
    20821 ttcaggagct attgattatg cgtaactttg atttatcccc gctttaccgt tctgctattg
    20881 gatttgaccg tttgtttaac cacttagaaa acaaccagag ccagagtaat ggcggctacc
    20941 ctccgtataa cgttgaactg gtagacgaaa accattaccg cattgctatc gctgtggctg
    21001 gttttgctga gagcgaactg gaaattaccg cccaggataa tctgctggtg gttaaaggtg
    21061 ctcacgccga cgaacaaaaa gagcgcacct atctgtacca gggcatcgct gaacgcaact
    21121 ttgaacgcaa attccagtta gctgagaaca ttcatgttcg tggcgctaac ctggtaaatg
    21181 gtttgctgta tatcgatctc gaacgcgtga ttccggaagc gaaaaaaccg cgccgtatcg
    21241 aaatcaacta attccctaag gccgcctggc gcggcctgac atctccatgc tcgccgtcag
    21301 ggagcatatg cgaatcttcg gatttgcagg tacttactcg cttcttagaa ggagaaatga
    21361 ctatgcgtaa cttcgattta tccccactga tgcgtcaatg gatcggtttt gacaaactgg
    21421 ccaacgcact gcaaaacgcc ggtgaaagcc agagcttccc gccgtacaac attgagaaaa
    21481 gcgacgataa ccactaccgc attacccttg cgctggcagg tttccgtcag gaagatttag
    21541 agattcaact ggaaggtacg cgcctgagcg taaaaggcac gccggagcag ccaaaagaag
    21601 agaaaaaatg gctgcatcaa gggcttatga atcagccatt tagcctgagc tttacgctgg
    21661 ctgaaaatat ggaagtctct ggcgcaacct tcgtaaacgg tttactgcat attgatttaa
    21721 ttcgtaatga gcctgaaccc atcgcagcgc agcgtatcgc tatcagcgaa cgtcccgcgt
    21781 taaatagcta actagctatt ctctttgccc cgccattctg gtggggcttt ttttgtcttt
    21841 cttcttcaac aatgcgttaa atttcgcctg tttaagatat ttcggcacgt tctttcccgt
    21901 gataatgtgc gctataaggc aaatctgctt caccgattca gccataatcc gtagcaatta
    21961 aaaatgttat ctcaagagaa atggacgatg agtgatatag cattaacggt cagtattctg
    22021 gctttggtgg cagtcgtcgg tttgtttatc ggcaatgtca aatttcgcgg cataggatta
    22081 ggtattggcg gcgtgctgtt tggtgggatc atcgtcggcc attttgtttc tcaggcgggg
    22141 atgacattaa gtagcgatat gctgcatgtt attcaggaat ttggcctgat cctgttcgtt
    22201 tatactatcg ggattcaggt agggccgggc ttctttgcct cattgcgcgt ctccggatta
    22261 cgcctcaacc tgtttgctgt tctgatcgtc atcatcggtg gtctggttac cgccatcctg
    22321 cataaactgt ttgatattcc actgccggta gtgctgggga ttttctccgg tgcggttacc
    22381 aatacgccag cgctgggggc agggcagcag attttgcgcg acctgggtac accaatggaa
    22441 atggtcgatc agatggggat gagttacgcg atggcgtatc cattcggcat ttgcgggatt
    22501 ttgttcacca tgtggatgtt gcgggttatt ttccgcgtca atgtcgagac agaagctcag
    22561 cagcacgagt cttcacgcac caatggcggc gcgctgatca agactatcaa tattcgcgtt
    22621 gagaacccta acctgcatga tttagccatt aaagatgtac cgattctcaa cggcgacaaa
    22681 attatctgct cgcgtctgaa acgcgaagaa accctaaaag ttccttcgcc agataccatt
    22741 attcaactgg gcgatttgct gcatctggtg ggtcagccag cggatttaca taatgcgcaa
    22801 ctggtgattg gtcaggaggt cgatacttcg ctgtccacga aaggcactga tttgcgcgtc
    22861 gagcgtgtgg tggtcaccaa tgaaaacgtg ctcggaaaac gtattcgcga cctgcacttt
    22921 aaagaacgct atgacgttgt tatctcgcgc ctgaaccgtg ccggggtcga actggtcgcc
    22981 agtggcgata tcagcctgca gttcggcgat atcctcaatc tggtggggcg tccgtccgca
    23041 attgatgccg ttgccaatgt gctggggaat gcgcagcaaa aactgcaaca ggttcagatg
    23101 ctgccagtgt ttattggcat cgggcttggc gtattgttag gttctattcc cgtctttgtg
    23161 cctgggttcc cggccgcgtt gaaactgggg ctggcgggcg gtccgctgat tatggcgttg
    23221 atcctcgggc gtatcggcag tatcggcaag ctgtactggt ttatgccgcc aagtgccaac
    23281 ctcgcgctgc gggagctggg gatcgtgctg ttcctctcgg tcgttggtct gaaatctggt
    23341 ggggattttg tgaataccct ggtcaatggc gaagggctaa gctggattgg ttatggtgcc
    23401 ctgatcaccg ccgttccgct gattactgtt ggcattctgg cgcggatgtt agccaaaatg
    23461 aattacctga ccatgtgcgg gatgctggca ggttccatga ccgatcctcc ggcgctggcg
    23521 tttgctaata atcttcatcc aaccagtggt gcggcggcgc tctcttacgc cactgtctat
    23581 ccgttagtga tgtttctgcg cattatcacc ccccaattac tggcggtgct cttctggagt
    23641 atcggttaac tcttttccgg gtggagtcgc cgtaaatggt actccacctg gtattcgctg
    23701 gcatttctga acatcactga ataattcaaa aactccccgc tctcgctata tgacaacgag
    23761 gtgatccgca gcagtggcgt gtgttccgct accttcattt gccgcgccag cagtctatcg
    23821 gcgagcaccg gcgtcaggct ttcataatta ccgccaatca aaatcccgca ctcttgtcca
    23881 atatattcaa acttcgaccc ttccagatgt tgcagcgaaa gattacggaa cagtttaacc
    23941 ggcatatagc tgtcttccag catcagcggt ttcccttcca caaaacgaac gcgacgggag
    24001 aagtagatct gctcgttgat ttgaatccgt aactggctgg caatggccgg aggcgcaggc
    24061 atgatttcaa aaatcagcac ctggctggtg accgtttttc cctgccgttt taacacctcc
    24121 accagtccgg tcaggctggc ggtttgatgc agcacatctt tgcgcaccag gtaagtgccg
    24181 ctgccgtggc ggcggaccac cagcccccac gctaccagca ggtcaatcgc tttacggatg
    24241 gtcatccggg atactgcaaa ttcttccgcc aacttttttt caccgggaag aaggctgttt
    24301 agcgtgaaat ccgcgtaatt aagtcgaatt cttaaccgct ccgcaatgct tttgtagatc
    24361 atctctagat ctcttttttg cctgcaagcc cgggtaatgc gcccacaagt tctgcttaat
    24421 cgattgaaaa aacatctttt ttttaaagat gtgttcgatg ctgtgtacca gtgctcacag
    24481 atgtctactt tttcgcgaaa acgtagatct ctaccgccca acgaaaagca tgaaagcgat
    24541 cacgaatccc attgggtcgt catgttctgc cgatcgcatc ttcctatcct cgctccaggc
    24601 ctgccgcata accaatcagg cttcctactt acagaattga gaaaagagga tgtggaaatg
    24661 ctcagtcaaa ttcaacgctt tggcggcgcg atgttcacgc cagtgctgct gtttcccttc
    24721 gccgggattg tggtgggtct tgccatcttg ctgcaaaacc cgatgtttgt cggggaatca
    24781 ctgaccgatc cgaacagttt attcgcgcaa atcgtacaca ttattgaaga gggcggttgg
    24841 acggtattcc gtaatatgcc gctgattttt gctgtcggtt tacccattgg ccttgctaag
    24901 caagcgcagg ggcgtgcttg tctggcggtg atggtgagtt tcctgacctg gaactatttc
    24961 atcaacgcga tgggaatgac ctggggaagc tacttcggcg tcgatttcac tcaggacgcg
    25021 gtggcaggta gcggtctgac aatgatggcc gggattaaaa ccctcgatac cagcattatc
    25081 ggcgcaatta tcatttccgg cattgtgacg gcgctgcata accgtctgtt cgataaaaaa
    25141 ctgccggttt ttctcggcat tttccaggga acgtcttatg tggtgattat cgccttcctg
    25201 gtgatgatcc cctgtgcctg gctgacattg ctcggctggc caaaagtaca aatggggatt
    25261 gaatctctgc aagcgttcct gcgttcggcg ggtgcacttg gggtctgggt ttacaccttc
    25321 ctcgaacgta ttctgatccc aaccggttta caccacttca tctacggacc gtttatcttt
    25381 ggtctggcag ctgttgaagg cggcattcag atgtactggg cgcagcatct gcaagagttc
    25441 agtctgagcg ccgagccgct gaaatcgttg ttcccggaag gcggttttgc cctgcacggt
    25501 aactcaaaaa tctttggtgc cgtgggcatt tctttagcga tgtacttcac tgccgcaccg
    25561 gaaaatcggg taaaagtggc gggcttgctg attcccgcaa ccttaaccgc catgctggtg
    25621 ggaattaccg aaccgctgga atttaccttc ctgttcattt caccgttgct gtttgcggta
    25681 cacgctgtgc ttgcggcctc aatgtcgacc gtgatgtatc tctttggtgt ggtgggcaac
    25741 atgggcggag gtctgattga ccaggtttta ccgcaaaact ggatcccgat gttcagcaac
    25801 cacgcggata tgatgctgac ccaaatcgcc attgggttgt gctttaccct gctgtacttc
    25861 gtggttttcc gcacactgat tctgcagttc aacatgtgca cgccgggacg tgaagatgcg
    25921 gaagtgaaac tctactcaaa agccgaatac aaagcctcgc gaggccaaac caccgcggca
    25981 gagccaaaaa aagagctgga tcaggctgcc ggtatcctgc aagccctggg cggggtcggc
    26041 aatatctcca gcattaacaa ttgtgcgacg cgtctacgca ttgcactgca tgacatgtca
    26101 caaacgctgg atgacgaagt ctttaaaaag ctgggagcgc acggcgtctt ccgtagtggc
    26161 gatgccattc aggtgatcat tggtctgcat gtatcccagc tgcatgaaca gctcgatagc
    26221 ttaattaatt ctcatcaatc agcagaaaat gttgccatta cggaggcagt ataatgacca
    26281 aattctcagt ggttgtcgca ggcggtggaa gcacctttac gccaggcatc gtgttgatgc
    26341 tcctggcgaa tcaggagcgt ttcccgcttc gtgcactgaa attttatgat aacgatggcg
    26401 cgcggcagga agtgattgcc gaagcctgta aagtcatcct taaagagaaa gcgccggaca
    26461 ttgcgtttag ttacaccacc gatcctgaag tggcgttcag cgacgttgat tttgtcatgg
    26521 cgcacatccg cgtcggcaag tacccgatgc gcgaactgga tgaaaaaatc ccactgcgcc
    26581 acggtgttgt tggtcaggaa acttgcggac ccggcggaat agcgtacggc atgcgttcca
    26641 ttggcggcgt cctggaactg gtggattata tggaaaaata ttcaccaaat gcctggatgc
    26701 tcaactactc caacccggca gccattgtcg cagaagccac gcgtcgtctg cgcccgaatg
    26761 cgaaaatcct caacatctgt gacatgccaa tcggtattga aagccggatg gcgcaaattg
    26821 ttgggctgca agatcgcaaa cagatgcgcg tgcgctacta cggcctgaac cactggtggt
    26881 cggcgatatc ccgcagtttc agaaagggtt aatgagtcag caagtggcgg tggaaaaact
    26941 ggtcgtggac gcctgggaac agcgttcata tcagcacctg tggcaggcga ttacgttgtc
    27001 gaaaacggta ccgagcgcct cggtcgccaa agctattctg gatgagttgc tggaggccaa
    27061 caaagcgtac tggccagagt tacgttaatt gtgtggactg gcatccgttg ggtgctggtc
    27121 cgtattcttt agcacggctg acggcgcata accaaattta tgtttaaagg ctttgctgaa
    27181 atgaaaggag tcgaagaagt tgagcgtatc agcgacttcg ccaaccgagt ttccttgttg
    27241 ctttaatagc gatagcgcca gatccagacg cgcatcgagg taatattctt tcggcgtctt
    27301 gccggtatag cgtaaaaaca gacgacgcaa ccaggcttcg ctacagggga tcgtggcagc
    27361 catatcagct acgctccagc gttgttgcag actggcatgt aaagtggcaa ttaatttttc
    27421 aatttgccgc cgttgtggat cttttttacc atctgcgtac ataagacaaa tccactgata
    27481 aataattttc gtcagaaaag cgactgccag attatttttt attgcttctg gtgaagttat
    27541 taactcagca acttcggtga gttcctgatt ataaatttcg ccattataaa taacgctttg
    27601 ctgaccaacg ggaatatcca tcatactggt gggggtaaat tccatccagt actgttccca
    27661 gactaaacct tcacagtgat aagagtgaat atccattggc tttaagaata taatacagtt
    27721 gccatgcagt gttatttgtt cgccattctt taaaagaatt ttcccacatc cttgtacggt
    27781 atacaccgca acccatgagt tggcgataag cggtttcttc ttgtcccgcg gccagatcac
    27841 ccggtaatgc tcgtcggcgt aggtatgcca gagcgagatc agagaaattc cgctggagat
    27901 cacattttcg ttaatcaata agggaatatt gtacggagtt tcgtttttta catctgaact
    27961 ttgcaatttt ccattcattt taatatccct ccggcgaatt atgctcacaa cctgaactta
    28021 acagaaaaca atccgttcag ggatacgacg tttgtatttt aagaatctga ctgcctgacc
    28081 cgacgcattt tacctctccc tatattcatg cgtccgggac ataaataagg gctatgagat
    28141 gaattcgtta caaatcttga gttttgtcgg ttttacgctg ctggtggcgg tgatcacctg
    28201 gtggaaggtc cgcaaaacag ataccggatc gcaacaaggc tattttcttg ccggacgttc
    28261 actaaaagcg ccggttattg ccgcttcgtt aatgctaacc aacctttcca cggaacaact
    28321 ggtcggcctt tccgggcagg cctacaaaag cggcatgtcg gtgatgggct gggaagtgac
    28381 ttcagcggtg acgctgatct tcctcgcgct aatcttttta ccgcgctatc tgaagcgcgg
    28441 cattgccacc atccccgatt ttctggagga acgttatgat aaaacgacgc gtattatcat
    28501 cgacttctgc ttcctcattg ccaccggcgt ctgctttctg ccgattgttc tctactccgg
    28561 cgcgttggcg ctcaacagcc tgtttcacgt cggggaatcg ctacagattt ctcacggtgc
    28621 ggctatctgg ctactggtaa ttttgcttgg tctggcggga attttgtatg cggtgatcgg
    28681 cggactgcgc gcaatggcag tggcggactc catcaacggt attgggctgg ttatcggcgg
    28741 gttgatggtg ccggtatttg gcctgatcgc gatgggcaag ggcagcttta tgcagggcat
    28801 tgagcaactc accaccgttc acgccgagaa attaaactca atcggtggcc cgaccgatcc
    28861 cttgccgatt ggcgcggcat ttaccggttt gattctggtg aacacctttt actggtgtac
    28921 aaatcagggc atcgtgcaac gcacgctggc gtcaaaaagc ctggcggaag ggcaaaaggg
    28981 ggcgctgtta acggcggtgc tgaaaatgct cgacccgctg gtactggtgc tgccagggtt
    29041 gattgcgttt catctgtatc aggatttacc gaaagccgac atggcctacc cgacgctggt
    29101 caataacgtt ctgccagtgc caatggtggg tttcttcggc gcggtgttat ttggtgcggt
    29161 gatcagtacc ttcaacggct ttctgaatag cgccagtacg ttattcagta tgggtattta
    29221 ccgtcgcatc attaaccaga atgccgagcc gcagcagctg gtcaccgtcg ggcgcaaatt
    29281 tggtttcttt atcgctatcg tttcggtgct ggtcgcgccg tggatcgcca acgcgccgca
    29341 ggggctgtat agctggatga aacagctcaa cggcatttac aacgtgccgc tggttaccat
    29401 catcattatg ggctttttct tcccgcgcat cccggcgctg gcggcaaaag tggcgatggg
    29461 gattggcata atcagctaca tcaccatcaa ctatctggtg aagttcgact tccatttcct
    29521 ctatgtgctg gcctgtacgt tctgcatcaa cgtggtcgtg atgctggtga tcggttttat
    29581 caaaccgcgc gccacgccgt tcaccttcaa agatgcgttt gcggtggaca tgaaaccgtg
    29641 gagaaacgtc aagatcgcgt caattggcat cctgttcgcg atgattggcg tctatgccgg
    29701 gctggctgaa ttcggcggct acggtacgcg ctggttagcg atgatcagtt atttcattgc
    29761 cgccgtagtg attgtctacc tgatttttga cagctggcgg catcgtcacg acccagccgt
    29821 aacctttact cccgacgcga aggatagcct atgaaacgcc ccaattttct gttcatcatg
    29881 accgataccc aggccaccaa tatggtcggt tgctatagcg gtaaaccgct gaatacgcaa
    29941 aatattgata gtctggcggc ggaaggtatt cgctttaatt ccgcctacac ctgttcaccg
    30001 gtttgtacgc ctgcacgcgc cggactattt accggtatct acgctaacca gtccggcccg
    30061 tggaccaaca acgtcgcgcc aggcaaaaac atctccacta tggggcgcta ctttaaggat
    30121 gccggctatc acacctgtta catcggcaaa tggcatctcg acggtcatga ctatttcggc
    30181 actggcgagt gtccgccgga gtgggacgct gattactggt tcgatggggc gaactacctt
    30241 agcgaactga cggaaaaaga gatcagcctg tggcgcaatg gcctaaacag cgttgaggat
    30301 ttacaggcga accatatcga cgaaaccttc acctgggcgc accgcatcag caatcgggcg
    30361 gtagattttc tgcaacagcc cgcgcgcgcc gaggaaccct tcctgatggt ggtttcgtat
    30421 gatgagccgc atcacccgtt cacctgtccg gtggagtatt tagagaaata cgctgatttt
    30481 tactacgatc tgggcgagaa agctcaggat gacctggcga acaaaccgga acatcaccgc
    30541 ttatgggcgc aggcgatgcc atcgccagtc ggtgatgacg ggctttatca ccatccgctc
    30601 tattttgcct gtaatgactt tgttgatgac caaatcggac gggtcatcaa tgccttaacg
    30661 ccagagcaac gtgaaaatac gtgggtcatt tatacttccg atcacggcga aatgatgggc
    30721 gcacataagc tgatcagtaa aggagcggcg atgtatgacg acatcacccg tattccgctg
    30781 atcatccgtt cgccgcaagg ggagcggcga caggtcgata cgccagtcag tcatatcgat
    30841 ttactgccga caatgatggc gctggcagat attgaaaaac cagagattct gccgggggaa
    30901 aatatccttg ccgtgaaaga gccacgcggt gtaatggtgg aatttaaccg ctacgagatt
    30961 gagcatgaca gctttggcgg ttttattccg gtgcgttgct gggtgacgga tgactttaaa
    31021 ctggtactca acctcttcac cagtgatgaa ctttacgatc gccgtaatga cccaaatgaa
    31081 atgcataacc tgatcgatga tatccgtttt gcagacgttc gcagcaaaat gcatgacgcc
    31141 ttattggatt acatggacaa aattcgcgat ccgttccgca gttaccaatg gagcctgcgt
    31201 ccgtggcgta aagatgcact gccgcgctgg atgggggcat ttcgtccacg cccacaagat
    31261 ggctattcgc cggtggtacg tgactatgac accggcctac cgacgcaagg agtgaaagtg
    31321 gaagagaaaa aacagaagtt ctgacgataa tgcccgctga tgaacacccg gcgggcatta
    31381 ttcaggctgc ttaaaccagt gaaccgccgg tcaggaagga aaaaatcagg aatatcagca
    31441 aaatgctgca cacgatgccg ataccaaaaa gcaaggtatg gaaagccagc gtcccgccgc
    31501 caaaaaccag cgcgccgcga atgccccagc gattttcctt acgcataccg ccaataaatg
    31561 cgcaaagtag cgcagcaatg gccagtgcga taccgctttg atcgaggatt ttatcgacat
    31621 ctacgtcctc ttttttctcc acggtggtaa ttttttcgcc ttttattccg gcgagaagac
    31681 ctcttttaat ttcagccgtt ttgtccgcca cgatgctttc aaacgttggc ggtggtgcgg
    31741 aaaaagggcc gaatgaaaag tggataatcc ccatcattaa agcgatggca ccgaacagca
    31801 tgcccaatga actgatttta ttttgagcta tgatacccat ctgacctcct tgtagcggat
    31861 ggcgaaagca tatcaggaaa cgggcggacg aaattcttaa aaaaaaaacg ctaaaggtag
    31921 agcgttagtg aaattgcaaa ggagcaacaa tgaaaatttc ccgccttgga gaagcgcccg
    31981 attaccgctt ctcgctggct aatgagcgta cttttctggc gtggatccgt accgcactag
    32041 gatttctggc ggcgggggtt gggcttgatc aacttgcgcc agatttcgcc acgcccgtca
    32101 ttcgtgaact gctggcgttg ttgttgtgtc tgttttccgg cgggttagcg atgtatggct
    32161 acctgcgctg gttacgcaat gaaaaagcga tgcgtctgaa agaggacctg ccctacacca
    32221 acagcttatt aatcattagc ttaatattga tggtcgtggc ggtgatcgtt atgggactgg
    32281 tgttgtatgc cggatagccg caaagccaga cgcattgccg acccaggact gcagccggag
    32341 cgcacatcac tggcgtggtt tcgtaccatg ctgggctacg gcgcgttgat ggcgttggct
    32401 atcaaacaca actggcacca ggcgggcatg ttattctgga tttcgattgg cattctcgcc
    32461 atcgtggcgc tgatcctctg gcactacacc cgtaatcgca atttaatgga tgtcacgaat
    32521 agcgattttt cccaatttca cgtagtccgt gacaaatttt tgatctccct cgcggtgtta
    32581 tctctcgcaa tactgtttgc tgtaacgcat atacatcaac ttatcgtatt tattgagaga
    32641 gtcgcatgac aggaagtcag gtcattgatg ctgaagagga caggcataaa ctggttgtag
    32701 agtacaagga tgctctgcaa cctgccgatt tttatcacaa tttcaaacag cggggcatcc
    32761 gctccgtaca gctcattccc tatcttgaat ttgatgaccg gggcgatctg acggccgctt
    32821 cggtgacagc agagctgtgg gggaaatttt taattgccct gttcgagtgt tgggtgcggg
    32881 cagatatcag tcgtatctcg attgagcttt ttgacgccac cttacaaaaa tggtgtggca
    32941 gcgaaaaccc gcaaccgcgc cgtgactgcc aggcgtgcga ctggcatcgc ctgtgcccgc
    33001 acgctcgcca ggagacgcca gacagcgtgc tttgtgcggg ctatcaggcg ttttattctt
    33061 actccgcacc gcatatgcgg gtgatgcgtg atctgataaa gcagcatcgt tctcccatgg
    33121 agctgatgac gatgctgcgg tgacgtgcgc ttaaacgggc tgcccctgat gcgacatccg
    33181 cgtcgccagc ggcagccagc acagcacgat caacaatccc attaaggtca tcaacaaccc
    33241 caggctgccc tgaccggttt gcggcaacat cgcagagagc gacgccagca cgccggaacc
    33301 aatgttttgc agaccgccga ccagcgcgcc agccgtgccc gccaggaagg ggaacggctc
    33361 catcgcgccg ctggtcgcca gcggaaacag catcccggca ccgaaaaaga acagcgcggc
    33421 gggaacgagc agcgtccaga cattcatcac gccaaaccag tcggggatcc acatcagcaa
    33481 gccagccagc aggcagcaga taaccgactg ccacattaac gtggagaagc gtttattggg
    33541 acgtccggca aaccatgcgc caaaaaacgc tgccggaatc ggcagaataa acaaaatact
    33601 gaccgtcata ctgctcagcc ctaacaccgc gcccatcagc acgccggagc aggcttcaaa
    33661 ggcggcaatc ccggccagac cgccaatcag catcagcaaa taacagttaa aaccgctgtt
    33721 accgaaaagc gttttataac tggtaagcag gcgcgtgcgc ggtgcatcga ccggacgcgt
    33781 ttccggcatc cagcgggcca tactgaaggt cacaccagca caaagaacca acaaaaagag
    33841 ataacaggcg cgccagttcc acatcgtatc cagcagaccg ccgattagcg gtgcgagcaa
    33901 cggactgacg agaatcccca tgtttaacag gctgttagca tggcgcaact gtgtccgttc
    33961 atataaatct cgcggcaaag tacgcgccat tacgccgcca acgccggtgc ccatcccctg
    34021 catcgcgctg gcggcaatca acaccgtcaa actggaggtc gtgaccgcga ccagcgttgc
    34081 cagcataaaa atggacattc cgacgaggat caccggtcgg cggcccacgc ggtcggaaat
    34141 cgggccataa aacagctgtg agacaccgta agtcagcaga taagcgccca ttacgctctg
    34201 caccgcccct tcacggacgt tgagatcgcg cgccatatcg gcaatagctg gaatataaat
    34261 ggtttgcgcc atctgaccga cggccacgag taataccaac atcaataaca aattgacgtt
    34321 tctttgcctt ttcattatca cggatgcttt tataaaaata attagaataa gtcactacgc
    34381 attcccataa atgcgggaga ggaatctatc acattgatga atgttagcca gatatacgcc
    34441 aggaatgggg aattgtttag cggcagaata tgtaaacaaa agcggcaata aatgttgccg
    34501 ggagggatta cgtgcgaact gtaaccctct ccccaaaggg gcgaggggga aaccaccgtt
    34561 ccctttcaga aaatgagcta aacattgcac gggacggtcc cctcgcccct ttggggagag
    34621 ggctagggtg aggggcacag gcagcgcagg tgcaaacatt ctgctggtag aagaagcccc
    34681 caactccaac ccgcacgcta aatataaaag gggagcggtt acccgctccc ctttggtgcg
    34741 acttgaatct gaattacttc aggtatttca gaacagcatc aagcagttgc agtgctgcaa
    34801 caatgagttt gaggataaga atggcgatat ccaccaggct catacgcgtc tcctgtggtt
    34861 caggagcact gccagctgac gtacctttcc gctgcctgtt gccagctgtt gtgcacgcgg
    34921 tatcttaagg agaaacgttg tgcttgttga cataacacag tgtgctcacg cggggttaag
    34981 gcgctgacgg caccacccgt ttcagccagg acttagtgcg ccgggtaacg atgcggcccc
    35041 cgcataacac attgcgtaca gtgatattat agaagtttac tgtataaata aacagtaata
    35101 tttggacaaa acgcaaactg tgatcgatac tcaactatgc cctaaatacg tcaaaattcg
    35161 tgccgaaatt gcgcgttctg cgcggaacac gtatactttc agtgttgaca taatacagtg
    35221 tgctttgcgg ttaccagccg caggcggctg atgaaacctc gctccgcggg gttttttgtt
    35281 atctgcaatt cagtacaaaa cgtgatcaac ccctcaattt tctcttgctg aaaaattttc
    35341 cattgtctcc cctgtaaagc tgtgcttgta taaatattgt taaacacaaa accaacaagg
    35401 tccccaatga ctacttccat gttcaacgca aaactactac caactgcgct atccgccgca
    35461 gtggtcgtcg tgcgtgtggt ggtggtcgtc ggcaatgcgc cgtagggact ggaacaacac
    35521 acgattccaa aaccccgccg gcgcaaaccg ggcggggttt ttcgtttaag cacctcccgg
    35581 aaagtcggcc cagaagaaaa ggactggagc atggcaagtt cgggcacaac attgacgcgt
    35641 aagcgcttta ccggcgcaga atttatcgtt catttcctgg aacagcaggg cattaagatt
    35701 gtgacaggca ttccgggcgg ttctatcctg cctgtttacg atgccttaag ccaaagcacg
    35761 caaatccgcc atattctggc ccgtcatgaa cagggcgcgg gatttatcgc tcaggggatg
    35821 gcgcgcaccg acggtaaacc ggcggtctgt atggcctgta gcggaccggg tgcgactaac
    35881 ctggtgaccg ccattgccga tgcgcggctg gactccatcc cgctgatttg catcaccggt
    35941 caggttcccg cctcgatgat cggcaccgac gccttccagg aagtggacac ctacggcatc
    36001 tctatcccca tcaccaaaca caactatctg gtcagacata tcgaagaact cccgcaggtc
    36061 atgagcgatg ccttccgcat tgcgcaatca ggccgcccag gcccggtgtg gatagacatt
    36121 cctaaggatg tgcaaacggc agtttttgag attgaaacac agcccgctat ggcagaaaaa
    36181 gccgccgccc ccgcctttag cgaagaaagc attcgtgacg cagcggcgat gattaacgct
    36241 gccaaacgcc cggtgcttta tctgggcggc ggtgtgatca atgcgcccgc acgggtgcgt
    36301 gaactggcgg agaaagcgca actgcctacc accatgactt taatggcgct gggcatgttg
    36361 ccaaaagcgc atccgttgtc gctgggtatg ctggggatgc acggcgtgcg cagcaccaac
    36421 tatattttgc aggaggcgga tttgttgata gtgctcggtg cgcgttttga tgaccgggcg
    36481 attggcaaaa ccgagcagtt ctgtccgaat gccaaaatta ttcatgtcga tatcgatcgc
    36541 gcagagctgg gtaaaatcaa gcagccgcat gtggcgattc aggcggatgt tgatgacgtg
    36601 ctggcgcaat tgatcccgca ggtggaagcg caaccgcgtg cagagtggca ccagttggta
    36661 gcggatttgc agcgcgaatt cccgtgtcca atcccgaaag cgtgcgatcc attaagccat
    36721 tacggcctga tcaacgccgt tgccgcctgt gtcgatgaca atgcgattat caccaccgat
    36781 gtgggtcagc atcagatgtg gaccgcgcaa gcttatccgc tcaatcgccc acgccagtgg
    36841 ctgacctccg gtgggctggg cacgatgggt tttggcctgc ctgcggcgat tggcgcggcg
    36901 ctggcgaacc cggatcgcaa agtgttgtgt ttctccggcg acggcagcct gatgatgaat
    36961 attcaggaga tggcgaccgc cagtgaaaat cagctggatg tcaaaatcat tctgatgaac
    37021 aacgaagcgc tggggctggt gcatcagcaa cagagtctgt tctacgagca aggcgttttt
    37081 gccgccacct atccgggaaa aatcaacttt atgcagattg ccgccggatt cggcctcgaa
    37141 acctgtgatt tgaataacga agccgatccg caggctgcat tgcaggaaat catcaatcgc
    37201 cctggcccgg cgctgatcca tgtgcgcatt gatgccgaag aaaaagttta cccgatggtg
    37261 ccgccaggtg cggcgaatac tgaaatggtg ggggaataag ccatgcaaaa cacaactcat
    37321 gacaacgtga ttctggagct caccgttcgc aaccatccgg gcgtaatgac ccacgtttgt
    37381 ggcctttttg cccgccgcgc ttttaacgtt gaaggcattc tttgtctgcc gattcaggac
    37441 agcgacaaaa gccatatctg gctactggtc aatgacgacc agcgtctgga gcagatgata
    37501 agccaaatcg ataagctgga agatgtggtg aaagtgcagc gtaatcagtc cgatccgact
    37561 atgtttaaca agattgcggt gttttttcag taaccgctca aggcttgaac aacatcgcgc
    37621 ttatcgttaa ggtaagcgcg ttttttttta cccgccagga caagaccatg atcaccgttg
    37681 cccttataga cgatcacctc atcgtccgct ccggctttgc gcagctgctg gggctggaac
    37741 ctgatttgca ggtagttgcc gagtttggtt cggggcgcga ggcgctggcg gggctgccgg
    37801 ggcgcggtgt gcaggtgtgt atttgcgata tctccatgcc cgatatctcc ggtctggaac
    37861 tgctaagcca gctgccgaaa ggtatggcga caataatgct ctctgttcat gacagtccgg
    37921 cgctggttga gcaggcgctt aacgcggggg cacgcggctt tctctccaaa cgctgtagcc
    37981 ctgacgaact gattgcggcg gtgcatacgg ttgccacggg cggctgttat ctgacgccgg
    38041 atattgccat taaactggca tccggtcgtc aggacccgct aaccaaacgc gaacggcagg
    38101 tggcggaaaa actggcgcaa ggaatggcgg tgaaagagat tgctgccgaa ctgggcttgt
    38161 cgccgaaaac ggtgcacgtc catcgcgcca acctgatgga aaaactgggt gtcagtaacg
    38221 acgtagagct ggcgcgccgc atgtttgatg gctggtgatg aagacgttgt tctcccgctt
    38281 aattaccgtt attgcctgct tttttatctt ctctgccgca tggttttgcc tgtggagtat
    38341 cagcctgcac ctggttgagc gccctgatat ggcggtgctg ttatttccgt ttggtctgcg
    38401 tctggggcta atgctgcaat gcccgcgcgg atactggccg gtgctgctgg gcgcggagtg
    38461 gctgctgatt tactggctaa cgcaggcggt cggtttaacc cattttccgt tattgatgat
    38521 cggtagttta ctgacgttac tgcccgtggc gctgatctcg cgctatcgcc atcagcgtga
    38581 ctggcgcacc ttgctgttac agggtgcggc gttaacggcg gcggcgttgt tgcagtcgct
    38641 gccctggctt tggcacggca aagagtcgtg gaatgcgctg ttgctgactt taactggcgg
    38701 cctgacgctg gccccgatat gtctggtgtt ctggcactat ctcgccaata acacctggct
    38761 gccgctcggc ccgtcactgg tttctcagcc gatcaactgg cgcgggcgac atctggtctg
    38821 gtacttgctg ctgtttgtta tcagtctctg gctccagttg ggattgccgg acgaactgtc
    38881 gcgctttacg ccattttgtc tggcgctgcc gattatcgcg ctggcctggc actatggttg
    38941 gcaaggggcg ctgattgcga cgttgatgaa cgccatcgcg ctgatcgcca gtcaaacctg
    39001 gcgcgatcat ccggtggatt tattgctctc gctgctggtg caaagtctga cagggttgtt
    39061 gcttggcgct ggcatccagc ggttgcgtga acttaaccag tcgctgcaaa aggaactggc
    39121 gcgcaatcag catctggctg aacggttgct ggaaaccgaa gagagcgtgc gccgtgatgt
    39181 ggcgcgtgag ctgcatgatg atatcggtca gaccatcaca gccattcgta ctcaggcggg
    39241 cattgttcag cggctggcgg cagataacgc cagcgtgaag cagagcgggc agctcatcga
    39301 acaactgtcg ctgggcgtgt acgacgcggt gcgccgttta ttaggacggt tacgtccgcg
    39361 ccagttggat gatctctccc tggagcaggc catccgctca ctgatgcggg aaatggagct
    39421 ggaagggcgc ggcattgtca gccatctcga atggcgaatc gatgaatcag cattaagtga
    39481 aaaccagcgc gtgacgctgt ttcgtgtctg ccaggaaggg ctgaacaaca ttgtgaaaca
    39541 tgctgatgcc agcgcggtca cgctgcaagg ctggcagcag gatgaacggt tgatgctggt
    39601 gattgaagac gatggcagcg gtttgccgcc gggttccggg caacaaggtt ttggcctcac
    39661 cggaatgcgc gagcgcgtaa cggcgctggg cggcacatta cacatttcct gtctgcacgg
    39721 cacgcgtgtc agcgtttctc tacctcaacg ctatgtctaa ggttcgatga tgttgccgtt
    39781 tctgaaagcg cctgccgatg cgccattaat gactgacaaa catgaaattg atgcccgcta
    39841 tcgctactgg cgtcggcata ttctgctgac catctggctg ggttacgcgc tgttttactt
    39901 cacgcgtaaa agttttaacg ccgccgtacc agaaatcctt gctaacggcg tgctcagccg
    39961 tagcgatatc ggcctgttag cgaccctgtt ttacattacc tacggcgtgt cgaagtttgt
    40021 ctccggcatt gtcagcgatc gctcaaatgc ccgttatttt atggggatag ggcttatcgc
    40081 cacgggcatc atcaacattc tgtttggctt ctcgacgtcg ctatgggcgt ttgccgtgct
    40141 ctgggtgctg aacgcctttt tccagggctg gggttcaccg gtgtgtgcgc gtctgttaac
    40201 ggcctggtat tcacgtaccg agcgcggcgg ttggtgggca ttatggaaca cggcgcataa
    40261 cgtcggcggc gcactcattc ccattgtgat ggcagcggct gcgctgcatt acggctggcg
    40321 tgccgggatg atgattgctg gttgtatggc gatagtcgtg gggatttttc tctgctggcg
    40381 gctacgcgat cgcccgcagg cgttaggttt accggcggtc ggtgaatggc gacacgacgc
    40441 gctggaaatt gctcaacaac aagaaggggc agggttgacg cgtaaagaga tcctcaccaa
    40501 atatgtgttg ctgaatccgt atatctggct gctttcgttt tgctatgtgc tggtctatgt
    40561 ggtccgggcg gcgatcaacg actggggcaa tttgtatatg tccgagacac tgggcgtcga
    40621 tctggtcacg gcgaatacgg cagtgacgat gtttgaactg ggcggattta tcggtgcgct
    40681 ggtagccggt tggggctcgg acaaattgtt taacggcaac cgtgggccga tgaatttgat
    40741 tttcgccgcc ggaattttgc tttcagtcgg ctcgctgtgg ctgatgccat ttgccagcta
    40801 cgtgatgcag gcaacctgct tcttcaccat tggttttttt gtctttggcc cgcagatgtt
    40861 aatcggcatg gcggcggcag agtgttccca caaagaggcg gcaggggcgg cgacggggtt
    40921 tgtcggcttg tttgcttatc tgggggcgtc gcttgctggt tggccgctgg cgaaagtact
    40981 cgatacctgg cactggagcg gattttttgt ggttatctct atcgccgccg ggatttccgc
    41041 actgctgtta ctgccctttt tgaacgccca ggcaccgcgc gaagcgtgat gcatctcacc
    41101 ttttcacttc atatccggca aaactaagaa attttccagg ttttgcctgg acgctatctc
    41161 aggcctgatt tgctgctgat ttttacaatg catgcctcac gcaggtattc atttcaggag
    41221 taacccatgc tggctttctt aaaccaggtt cgcaagccga ccctggacct tccgctcgaa
    41281 gtgcggcgca aaatgtggtt caaaccgttc atgcaatcct acctggtggt ctttatcggc
    41341 tacctgacga tgtacctgat tcgcaagaac tttaacatcg cgcagaacga catgatctcg
    41401 acctacgggt tgagcatgac gcagctgggg atgatcggcc tgggtttctc catcacttat
    41461 ggcgtgggta aaacgctggt ttcctactac gccgacggca aaaacaccaa acaattcctg
    41521 ccgttcatgc tgatcctgtc tgccatttgt atgctgggct tcagtgccag tatgggcagc
    41581 ggctcggtta gcctgttcct gatgattgcc ttctacgcct taagcggctt tttccagagt
    41641 accggcggtt cgtgcagtta ctccaccatc accaaatgga cgccgcgtcg taaacgcggg
    41701 acattcctcg gtttctggaa tatttctcac aaccttggcg gtgcaggcgc agcaggtgtg
    41761 gcgctgttcg gggcaaatta cctgttcgat ggccatgtca tcggcatgtt tatcttcccg
    41821 tcgattatcg cgctgattgt cggttttatc ggcctgcgtt acggcagcga ctccccggaa
    41881 tcttatggcc tcggcaaagc tgaagaactg ttcggcgagg agatcagcga agaggacaaa
    41941 gagacagaat ctaccgatat gaccaagtgg cagatctttg ttgagtatgt gctgaaaaac
    42001 aaagtgatct ggctgctgtg cttcgccaac attttcctct atgtggtacg tattggtatc
    42061 gaccagtggt caaccgtata cgcgttccag gaactgaaac tctctaaagc ggtggcgatt
    42121 cagggcttta cgctgtttga agctggtgcg ctggtcggta cgctgctgtg gggctggctc
    42181 tctgacctgg cgaacggtcg ccgtggcctg gtggcctgca tcgcgctggc gctgattatc
    42241 gccacgctcg gtgtgtatca acatgccagc aacgaatata tctatctggc ttctctcttt
    42301 gcgttgggtt tcctggtctt tggcccgcaa ttgttgattg gtgtggctgc tgttggcttt
    42361 gtacctaaaa aagcgattgg cgctgccgat ggtattaaag gcacctttgc ttacctgatt
    42421 ggtgacagct ttgccaagtt aggtctggga atgattgccg atgggacgcc ggtattcggc
    42481 cttaccggct gggcaggcac cttcgccgca cttgatatcg ccgcgattgg ttgtatctgc
    42541 ctgatggcga tagtggcggt gatggaagaa cgcaaaatcc gccgcgagaa aaaaattcag
    42601 cagttgacag tggcataaac gtaactggtg acttttgccc ggcatgacgc cgggcttttt
    42661 tattattccg tgacttccag cgtagtgaag gcaaacttct cgccatcaaa tagcccctga
    42721 ctggttagtt ttagcgcggg gatcactggc agagaaagaa acgccatctg aataaacggc
    42781 tcatcgggta acggaccgca ttcacgggcg gcggctttca aggcgtcaat ttgttccgcc
    42841 agcgactgcg ccgtgtcggt gctcatcagg ccggcaatgg gcaacggcag atgactttgg
    42901 acctggccgt tacgtaccac gcacagcccg ccgccatcct gaatcacctg attgaccgcc
    42961 agcgccatct cttcggcact gcgaccgatc accacaatat tatggctgtc atggctgacc
    43021 gtcgcagcca gcgcaccttc attcagtcca aagccgccaa gcaaaccaca agccggagcc
    43081 agccgttgcc cgtaacgctc aagtacggca ataaagctca catcatcgcg atcaaaacca
    43141 ttttcgctgt agacgctgga gtgggagtgc gtaatcaatt cgttatggat gacgtcaatg
    43201 acccgatagc gttttccggg cgtaaattgc agggcaaagt cgctggcgga aactggctgg
    43261 cgggcaatgg tgttgccata tggcggagcg gattgtgcca gtctcgccga ctcttccgcc
    43321 tgtaaggttt gcgcatcaat cggctcgcct ttcaccagta cctgctgcac cgtgacctta
    43381 cgcgcatcgc tcaacaggac gatatcggcc tgcttgccgg gtgccagtaa gccgaggtga
    43441 ttcagaccaa agtggcgcgc cgtcgaccag ctggcgacgc gatatgccac atgcagcggc
    43501 acattgtgtt gttcgatcag gcggcgaatt aaggcatcga tgtgtccttc atgggcgatc
    43561 tcccacgggt tacggtcatc ggtacagagc atgcattgcg ggctgttaaa ttcgttgatc
    43621 aacggtgcca gcgcgttgag attgcgggca gcggaccctt cgcggatcat caacgacatg
    43681 ccgagttgta atttccggcg tccttcttcc agctgataac tttcgtggca gttttcaata
    43741 cccgcagtaa tataggcgtt aagttcttta ccacccaaac ccgggcagtg accgtccagc
    43801 gtcaggtggc gaaatgcatc cagtttatcg agcagcgcat tctgcccgct aattacgcca
    43861 gggtagtcca tcatttctgc aaggccggta acctgcggat ggtcccgcca ggcgagcatc
    43921 tgttcaaggg taaaactggc accgttaaca tcgcagcctt ccagggcggg tacgcaagag
    43981 ctgacctgta agtactggtt ttgccttgcc tgttcggcac agcgggcaaa ccaggcgaat
    44041 ccggcttctc ccatcacgtt gacgatttca tgagggtcgc aaataacggt cgtcaggccg
    44101 cgcggcaggg tagcggtttc aaaagtgacc ggcgtcatca tgctggattc aatatgcagg
    44161 tgagcatcaa taaaccctgg caccgccgtt gcgccgcgag catcaatccg ctgcaaagcc
    44221 ggagcatcag cgtattctgc gccaacaccg gcaatgtaac gtcctttaat cacaattggg
    44281 ccggaaattt ctccgccatt gatcaggtcg agaatagaga cattatcaat aatataatcg
    44341 gcaacagcgt cgccacggga aacggctaac aattcctggt attcagcccg gctaatgtga
    44401 tgaaatttat ggttaataga attattcata ttttt
//
LOCUS       DAPHDX010000022        43668 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPHDX010000022 DAPHDX010000000
VERSION     DAPHDX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.022"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2680)
                     /gene="rhsB"
                     /locus_tag="RH058_001169"
     CDS             complement(<1..2680)
                     /gene="rhsB"
                     /locus_tag="RH058_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV3136788.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDS
                     LDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRY
                     RYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISEGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRD
                     YTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAA"
     gene            complement(2840..3052)
                     /gene="rpmE"
                     /locus_tag="RH058_001170"
     CDS             complement(2840..3052)
                     /gene="rpmE"
                     /locus_tag="RH058_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV3136789.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            3255..5453
                     /gene="priA"
                     /locus_tag="RH058_001171"
     CDS             3255..5453
                     /gene="priA"
                     /locus_tag="RH058_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV3136790.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNGLKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            5609..6634
                     /gene="cytR"
                     /locus_tag="RH058_001172"
     CDS             5609..6634
                     /gene="cytR"
                     /locus_tag="RH058_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV3136791.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            6726..7685
                     /gene="ftsN"
                     /locus_tag="RH058_001173"
     CDS             6726..7685
                     /gene="ftsN"
                     /locus_tag="RH058_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV3136792.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            7778..8308
                     /gene="hslV"
                     /locus_tag="RH058_001174"
     CDS             7778..8308
                     /gene="hslV"
                     /locus_tag="RH058_001174"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV3136793.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            8318..9649
                     /gene="hslU"
                     /locus_tag="RH058_001175"
     CDS             8318..9649
                     /gene="hslU"
                     /locus_tag="RH058_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV3136794.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            9716..10642
                     /gene="menA"
                     /locus_tag="RH058_001176"
     CDS             9716..10642
                     /gene="menA"
                     /locus_tag="RH058_001176"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV3136795.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            10735..11220
                     /gene="rraA"
                     /locus_tag="RH058_001177"
     CDS             10735..11220
                     /gene="rraA"
                     /locus_tag="RH058_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV3136796.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(11305..11550)
                     /gene="zapB"
                     /locus_tag="RH058_001178"
     CDS             complement(11305..11550)
                     /gene="zapB"
                     /locus_tag="RH058_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV3136797.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            11975..12820
                     /gene="glpF"
                     /locus_tag="RH058_001179"
     CDS             11975..12820
                     /gene="glpF"
                     /locus_tag="RH058_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV3136798.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVSGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            12843..14351
                     /gene="glpK"
                     /locus_tag="RH058_001180"
     CDS             12843..14351
                     /gene="glpK"
                     /locus_tag="RH058_001180"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV3136799.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            14487..15497
                     /gene="glpX"
                     /locus_tag="RH058_001181"
     CDS             14487..15497
                     /gene="glpX"
                     /locus_tag="RH058_001181"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV3136800.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            15594..16340
                     /gene="fpr"
                     /locus_tag="RH058_001182"
     CDS             15594..16340
                     /gene="fpr"
                     /locus_tag="RH058_001182"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV3136801.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(16345..16773)
                     /gene="uspD"
                     /locus_tag="RH058_001183"
     CDS             complement(16345..16773)
                     /gene="uspD"
                     /locus_tag="RH058_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV3136802.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(16800..17099)
                     /locus_tag="RH058_001184"
     CDS             complement(16800..17099)
                     /locus_tag="RH058_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV3136803.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(17311..17751)
                     /locus_tag="RH058_001185"
     CDS             complement(17311..17751)
                     /locus_tag="RH058_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3136804.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWLAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            17852..18451
                     /locus_tag="RH058_001186"
     CDS             17852..18451
                     /locus_tag="RH058_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV3136805.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            18559..19326
                     /gene="tpiA"
                     /locus_tag="RH058_001187"
     CDS             18559..19326
                     /gene="tpiA"
                     /locus_tag="RH058_001187"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV3136806.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(19381..20136)
                     /gene="cdh"
                     /locus_tag="RH058_001188"
     CDS             complement(19381..20136)
                     /gene="cdh"
                     /locus_tag="RH058_001188"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV3136807.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(20243..21232)
                     /gene="sbp"
                     /locus_tag="RH058_001189"
     CDS             complement(20243..21232)
                     /gene="sbp"
                     /locus_tag="RH058_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV3136808.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(21552..22514)
                     /gene="pfkA"
                     /locus_tag="RH058_001190"
     CDS             complement(21552..22514)
                     /gene="pfkA"
                     /locus_tag="RH058_001190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV3136809.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(22695..23597)
                     /gene="fieF"
                     /locus_tag="RH058_001191"
     CDS             complement(22695..23597)
                     /gene="fieF"
                     /locus_tag="RH058_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV3136810.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(23805..24446)
                     /locus_tag="RH058_001192"
     CDS             complement(23805..24446)
                     /locus_tag="RH058_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136811.1"
                     /translation="MEKVIIELDNRWELADFAVLTKEYLQLYGFFYGLKGGSRGYSTM
                     PWEGGHSVVNFFRGAYSATPPDLRPVVKKIQYASPGFIELSALIDISWQIAELVTAVG
                     GSILAANKVYDQVMRTYRQREWAKLKSEKLRIQNQIKEIELVSDAVKSLESVMALSEE
                     QRKNLVQLSGADELVQLKILLAVYRRLSPLVELQNSGKANFSAGKNKNLKASD"
     gene            complement(24587..24805)
                     /gene="ogrK"
                     /locus_tag="RH058_001193"
     CDS             complement(24587..24805)
                     /gene="ogrK"
                     /locus_tag="RH058_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HDV3136812.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(24887..25012)
                     /locus_tag="RH058_001194"
     CDS             complement(24887..25012)
                     /locus_tag="RH058_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control D family protein"
                     /protein_id="HDV3136813.1"
                     /translation="MDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            25011..26765
                     /locus_tag="RH058_001195"
     CDS             25011..26765
                     /locus_tag="RH058_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDV3136814.1"
                     /translation="MTRLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSWKQRDGW
                     DSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQTGNEAD
                     LNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHRIRDILK
                     SRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVDLTGDPI
                     VLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSHLRSTYF
                     STPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQIVTIED
                     ALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTLEEWEDY
                     APFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGMDFATQA
                     ESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVLKAKDVI
                     RRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATMHALLNE
                     PLTAGISTPLTSTILEFY"
     gene            26765..27799
                     /locus_tag="RH058_001196"
     CDS             26765..27799
                     /locus_tag="RH058_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV3136815.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFTPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(28882..31158)
                     /locus_tag="RH058_001197"
     CDS             complement(28882..31158)
                     /locus_tag="RH058_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDV3136816.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWSDPEVVMALRGALKHGLRTPNRQ
                     QRNGIPLKPHEIAPSARLTRSERLQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSLPI"
     gene            complement(31148..31423)
                     /locus_tag="RH058_001198"
     CDS             complement(31148..31423)
                     /locus_tag="RH058_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDV3136817.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSSLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            complement(31420..31503)
                     /locus_tag="RH058_001199"
                     /pseudo
     CDS             complement(31420..31503)
                     /locus_tag="RH058_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
     gene            31492..32088
                     /locus_tag="RH058_001200"
                     /pseudo
     CDS             31492..32088
                     /locus_tag="RH058_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(32275..32775)
                     /gene="cpxP"
                     /locus_tag="RH058_001201"
     CDS             complement(32275..32775)
                     /gene="cpxP"
                     /locus_tag="RH058_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV3136818.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            32925..33623
                     /gene="cpxR"
                     /locus_tag="RH058_001202"
     CDS             32925..33623
                     /gene="cpxR"
                     /locus_tag="RH058_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV3136819.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            33620..34993
                     /gene="cpxA"
                     /locus_tag="RH058_001203"
     CDS             33620..34993
                     /gene="cpxA"
                     /locus_tag="RH058_001203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV3136820.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(35099..35773)
                     /gene="yiiM"
                     /locus_tag="RH058_001204"
     CDS             complement(35099..35773)
                     /gene="yiiM"
                     /locus_tag="RH058_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV3136821.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(35922..36905)
                     /gene="kdgT"
                     /locus_tag="RH058_001205"
     CDS             complement(35922..36905)
                     /gene="kdgT"
                     /locus_tag="RH058_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV3136822.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(37165..37785)
                     /gene="sodA"
                     /locus_tag="RH058_001206"
     CDS             complement(37165..37785)
                     /gene="sodA"
                     /locus_tag="RH058_001206"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV3136823.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            38070..39032
                     /gene="rhaT"
                     /locus_tag="RH058_001207"
                     /pseudo
     CDS             38070..39032
                     /gene="rhaT"
                     /locus_tag="RH058_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(39030..39194)
                     /locus_tag="RH058_001208"
                     /pseudo
     CDS             complement(39030..39194)
                     /locus_tag="RH058_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            39482..40951
                     /gene="rhaB"
                     /locus_tag="RH058_001209"
     CDS             39482..40951
                     /gene="rhaB"
                     /locus_tag="RH058_001209"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV3136824.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            40948..42207
                     /gene="rhaA"
                     /locus_tag="RH058_001210"
     CDS             40948..42207
                     /gene="rhaA"
                     /locus_tag="RH058_001210"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV3136825.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLSWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            42449..43273
                     /gene="rhaD"
                     /locus_tag="RH058_001211"
     CDS             42449..43273
                     /gene="rhaD"
                     /locus_tag="RH058_001211"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV3136826.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            43283..43597
                     /gene="rhaM"
                     /locus_tag="RH058_001212"
     CDS             43283..43597
                     /gene="rhaM"
                     /locus_tag="RH058_001212"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV3136827.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        10526 a        11588 c        11261 g        10293 t
ORIGIN      
        1 tcgctgcggt ggtgtgaacg ccggtcagcc tgccggtggt gctgtagctg taactccggg
       61 tctggcgcgg gctgctgatg cggatgagtt cgccgttgtc gttccaggtg taatcgcggt
      121 cagacagcag gctgttcagg tgctggctct gtaactgccc ggcaggggta taagcggtgg
      181 tgagttcata acggccgaag ctgcgcagcg tttcccggtg caggcggtcg cgggtgtact
      241 ccaccagcgg tgtgtcgccg agtttcatgc ctgccaggta accgctgccg taggtcagcc
      301 attccacggc gggcaggctg tccggtatac agcggttcgc cagcccctgc gcgttgtacg
      361 catgtctggt ctcatgctgc cagagcagtg cttccgtctg cgggtgatgc accgtctgac
      421 gctcaccggt cagccggcct ttctcatcat acccgtaatg caccgtcacc cggtgccctt
      481 cgctgatatg gctgatgtct gtcagccagc cgcgttcgtc atactgccac tgctctgcgg
      541 tttcgccatt cacggtgcgg tgcgtgaggc ggtctgcttc gtcatagtgc cagtgggtga
      601 ccagcccctc atcctcgctg cggataagtt tgccggtcag gtcgtggtga taacgctgtg
      661 tgcggccgtc aaagccggtt tcctgtatca gccggtcgag tacatcgtaa cggaaggtgg
      721 tgtggctgcc gttttcactg gtcaggcgga tgacccgtcc ggcagcatcg tattccatac
      781 tgcgcgtcag cccgccctgc gtggtacaga tggcctttcc ccacgcatcg tactgtgtcc
      841 cgtttctgct gccgtccggg gcaatgacgg cggtcaggtc accggcgatg ttgtattcat
      901 accgcgtttc atggccctgc gtgtctttca cggcaattaa ctgtccacgg ctgtcgtatg
      961 cgcggtactg actcagccct tcctcgcggt gcaccgccgt cacctgacca aaacggtcat
     1021 ggtcatagcg ggttacataa ccggaacagt cagtgaagct cagcaactga ccgtaacggc
     1081 tccacgtcat ggttttccgg ctgccggtgg catcgtccgt tgcgcagggg aggtcactgt
     1141 gtggattatc ataacggtag cgggtgatat cgccgtcagg ggcggtttcc tgaatcagac
     1201 gtccgtactc atcatatttg cggcgcattt ccagcccgtc tggcccggtg gctgacgtta
     1261 actggctgtg gtggttatag taaaacgccg atgccctgcc atccggcgtg gtgatgcgcg
     1321 tgatgaggcc cgtcaccaca tccgggctgt actctgttgt ccggcctgcg gcatccgtct
     1381 gtgccttaag cctgcccacc gcatcaaact gactctgcgt gacgctgccg tccgcgtgtt
     1441 cctttttcac cacccgcttc agcccggctt cgccctgcgt gtgcagcact tcacggcggt
     1501 ccaggctgtc ggtgatggtg atgtggtctt tctcatactg atacgtgtag cttaagcctg
     1561 ccgggttaag ctgttctgtc acccgcccgt cgctgtcgta acggtaacgg atttccggcc
     1621 ggcccgtgtg acggtgcgcc accatccggc cccggtattt atcatcgtaa gtaaagctgc
     1681 gcacctgttt gccactacgg tcatacacca ccgccagttc gccgcgtggc gtccagccat
     1741 agcgcaccag cggcgcggca ggtaaattct ccgggtattc cgggtcgtgc gtcagccaca
     1801 cggcagacag acggatgccg ttgtcccggc catattcggt ataacccggc agggtatcag
     1861 gaaaagcgga cggttccgtc ccgccggaaa tggcctgctg ccgggcttct tctgcccgct
     1921 gcgcctgcgt ggtcagtacc agccggaagt gacgcccggc accatccgtc acgccggtga
     1981 tttcgccgct gaattcaccg gcggcttcgc ggtggaacgt ctgtgtgcgc ccgaagcggt
     2041 ccaccagccc ggtcagtacc cggtacggcg gcagcggcgc aggcagcacc tcatccgctt
     2101 ccggcacccg ctcacaccag ccgagcagcc accacggccc ctgcggactg tttgtcgcca
     2161 gataacgatg cggacttaag cggagttctt ccggcagcgc ctgccagagt gcggccagcc
     2221 ggtgaccttc atccagtttc gccacgccgc cgcgcaccag ccacagtgac tcgctgcggc
     2281 tgtaaccgtc ctcaccggga aacaggtgct caaaatacag gcttctgccg ccgttatcac
     2341 tgagtatcag tgtgttatcg cgcagctgta agcggatatc cgcaggcatt ttccagccgg
     2401 ggccgaggct ccccaccggc gcgggcgttt ttgtccggta actgctgtag gtgcgggaga
     2461 gaatgaacgg cagcgggccg ggcagggcga tgtcggtttc accgggaagg acctttgcac
     2521 cgagcagggg attgaccgga tggccggacg tcactccgcc ggggcacacc gaacaggcca
     2581 cgccggtggg ggcaccaatg cgcaccccgg ctgaaccctg aacaatgcta ccgccatact
     2641 gcgtcatgtc gccctgacgc gctgccggtt ttccgctcat cgtaaaatcc ttttgaaata
     2701 aataagaacc agaagcctag cgccagtcaa caacaggaaa tgataatgag ttaacacccc
     2761 ggaatttttc agaatttata aaaggcaaat tacaggcaca aaaaaagcgc cgtgcggcgc
     2821 ttttttcgga aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt
     2881 caacacggcc accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc
     2941 tgcacacgtc gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc
     3001 aagagcagct agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac
     3061 ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa
     3121 gtatagcttc aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc
     3181 gtatgcaaac tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa
     3241 gtcaggatga tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct
     3301 ttgactatct gctgccagaa ggcatgacgg ttaaagccgg gtgtcgcgtg cgcgtgccgt
     3361 ttggcaaaca gcaggagcgc atcggggttg tggtgtcagt tagcgatgtc agtgagctgc
     3421 cgcttaacgg gctgaaagcg gtagtggaag tgctggacgt tgagccggta tttactcact
     3481 ccgtctggcg gttactgctc tgggcggcag attactatca tcatccgatt ggcgatgtgc
     3541 tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc tgcggcgaac gcgccgatgt
     3601 ggtactggtt tgccactgaa caaggccagg cggtggatct gaacagcctg aaacgctccc
     3661 ccaagcaaca acaggcgctg gcggcgttac ggcaaggcaa aatctggcgc gaccaggtcg
     3721 ccacgctcga atttaatgat gccgcgttgc aggcgctacg caaaaaaggt ctgtgtgatt
     3781 tagcaagtga aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc
     3841 ggttgcgatt gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata
     3901 ctttttctgc ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca
     3961 gtgtactgga aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg
     4021 gcctgacacc gcaaactatc gcccgttttc gtgaacgttt taatgccccc gtggaagttc
     4081 tgcattccgg cctgaacgac agcgagcggc tttcggcgtg gttgaaagcg aaaaatggcg
     4141 aggcggcgat tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg
     4201 tgattgtcat tgatgaagag cacgacagct cctacaagca gcaggaaggc tggcgctatc
     4261 atgcccgcga cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct
     4321 ccgcaacgcc cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc
     4381 gcctgacgcg ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag
     4441 gtcagaaggt gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac
     4501 aggccaacaa tcaggttatc ctctttctta atcgtcgggg ttttgcgcct gcgctactgt
     4561 gccacgactg cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc
     4621 aggcgcaaca gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt
     4681 gcccttcctg cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac
     4741 agacgcttgc gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca
     4801 gccgcaaagg ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt
     4861 tgatcggcac acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt
     4921 tactggatgt ggacggcgcg ctgttttctg ccgattttcg ctcggcagaa cgtttcgccc
     4981 agctttacac ccaggtcgcc ggtcgtgccg ggcgtgcggg taaacagggc gaagtggtgc
     5041 tgcaaacgca ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg
     5101 cctttgccga acaggcgctg gctgagcgac gcatgatgca gctgccgccg tggaccagcc
     5161 atgtgattgt gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac
     5221 tgcgtaatct gatcctttcc agcccgctgg cagacgacaa actgtgggtt ctcggcccgg
     5281 ttccggctct ggcacctaag cgtggtggcc gctggcgctg gcagatcttg ttgcagcacc
     5341 cttcccgcgt gcgcttgcaa cacatcatta gtggtacgct ggcgcttatt aatacaatac
     5401 cggattcccg caaggtgaaa tgggtgttgg atgtcgatcc catcgaaggc taatgcgcaa
     5461 acgttgcgag gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa
     5521 ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta
     5581 cgcctgacgt gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac
     5641 catgaaagac gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat
     5701 gaatcccgat aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt
     5761 gggttattta ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct
     5821 ggtgattgtc ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt
     5881 tacggcggca aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca
     5941 ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg
     6001 ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat
     6061 ggcgaacgaa ttcgcaccgg aactggagct acctacagtt catatcgaca atctgaccgc
     6121 cgcatttgat gcagtaaatt atctatatga gcaagggcat aaacggattg gctgtatagc
     6181 cggtcccgaa gagatgccgc tgtgtcacta ccgcctgcaa ggttacgttc aggcgctgcg
     6241 tcgctgcggc attatggttg atccgcaata catcgcccgt ggcgacttca ccttcgaagc
     6301 cggaagcaaa gcgatgcagc agctgcttga tcttccacaa ccgcctactg ctgtcttctg
     6361 ccatagcgat gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt
     6421 cccggaagac ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc
     6481 gccgctgaca accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt
     6541 gcttgatcaa atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact
     6601 tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact
     6661 ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa
     6721 cgatagtggc acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa
     6781 agagcacctc acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta
     6841 ttgctgccgc cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga
     6901 aagaagagtc cgagacgctg caaagccaga aagtgaccgg taacggacta ccaccaaaac
     6961 cagaagaacg ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc
     7021 ccacagaacc ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac
     7081 gtcagcttct tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgaag
     7141 ttccgtggaa tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg
     7201 cgcagcaatt agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct
     7261 ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta
     7321 aaccggcttc tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg
     7381 cgcagtcgaa gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga
     7441 cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg
     7501 aacaggcaga gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca
     7561 ccaacaatgg ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca
     7621 gcaccctcaa tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg
     7681 gttgaaaccc tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg
     7741 ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg
     7801 ccgtaacggc catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat
     7861 gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc
     7921 gggcggtact gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca
     7981 tcagggccat ctggtcaaag ccgccgttga gctggcgaaa gactggcgta ccgatcgcat
     8041 gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac
     8101 cggtaacggt gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc
     8161 ttacgcccag gctgcggcgc gtgcactgtt agaaaatact gaactgagtg ctcgtgaaat
     8221 tgctgaaaag gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac
     8281 catcgaagaa ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga
     8341 aatcgtcagc gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc
     8401 gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt
     8461 gaccccgaaa aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg
     8521 tcgtctggct aagctggcga atgcgccgtt catcaaagtt gaagcgacca aattcaccga
     8581 agtgggctac gtcggtaagg aagtggattc tattattcgc gatctgaccg atgccgccgt
     8641 gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga
     8701 agaacgtatt ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agaccgaaca
     8761 gcagcaggaa ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct
     8821 tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc
     8881 tcctccaggc atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg
     8941 ccagaagcaa aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga
     9001 agaagcggcg aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga
     9061 gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc
     9121 cggtccggat gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg
     9181 caccgtttcc accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg
     9241 cgcgttccag attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc gtctgccaat
     9301 ccgcgttgaa ctgcaggcgc tgaccaccag cgacttcgag cgtattctga ccgagccgaa
     9361 tgcctctatc accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt
     9421 taccgactcc ggtattaagc gcatcgcgga agcggcatgg caggtgaacg aatctaccga
     9481 aaacatcggt gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta
     9541 cgacgccagc gatttaagcg gtcaaactat catcattgac gcagattatg tgagcaaaca
     9601 tctggatgcg ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat
     9661 cattttcatc attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac
     9721 tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc
     9781 cctcgccttt gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga
     9841 tccgctggtc gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct
     9901 cgccaatgat tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct
     9961 acgcggcatg caaaaagggg tcattaccca gcaagagatg aaacgggcgc tcattattac
    10021 cgtcgtgctc atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc
    10081 cgattttgtc ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac
    10141 cgtgggcaat cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt
    10201 tggctggttg agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact
    10261 gatccttccg gcgaccgcct gtggcctgct ggcaacggca gtactgaata ttaataacct
    10321 gcgtgatatc aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga
    10381 agtgaacgcg cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct
    10441 gtttaatctc ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt
    10501 actggtgaag caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat
    10561 gctggaacgt actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt
    10621 cctaagccag tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac
    10681 agcctacata gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa
    10741 tacgatactt cggagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg
    10801 ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc
    10861 gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc
    10921 gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca
    10981 gtacaaaatg aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg
    11041 gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa
    11101 ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac
    11161 catctttatg ccgacaatac cgggattatt ctttctgaag atccgctgga tattgaatga
    11221 tagaaaaggc accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga
    11281 aattacgcgg aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag
    11341 acgttcctgc cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc
    11401 gcgctgatgc tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc
    11461 ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag
    11521 tttctcaaac acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga
    11581 taaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    11641 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    11701 gccacacttt tcatccttct cctggtgaca taatccacat caatcgaaaa tgttaataaa
    11761 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    11821 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    11881 ctcgttaacg ataagtttac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    11941 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    12001 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt ttcttcggtg tgggttgcgt
    12061 tgcagcacta aaagtctctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    12121 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    12181 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    12241 tatcgtttca caagttgccg gcgctttctg tgctgcggct ttagtttacg ggctttacta
    12301 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    12361 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    12421 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggatga
    12481 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    12541 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    12601 tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    12661 cattccttac ttcctggtgc cgcttttcgg ccctatcgtt ggcgcgattg taggtgcatt
    12721 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    12781 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    12841 acatgactga aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg
    12901 tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct
    12961 acccaaaacc aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca
    13021 cgctggtaga agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta
    13081 ttacgaacca gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata
    13141 acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg
    13201 gtttagaaga ttatatccgc agcaataccg gtctggtgat tgacccgtac ttttctggca
    13261 ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg
    13321 aattgctgtt tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc
    13381 atgtgaccga ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact
    13441 gggacgacaa aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc
    13501 gttcttccga agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa
    13561 tctccgggat cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag
    13621 ggatggcgaa gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag
    13681 cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga
    13741 actatgcgtt ggaaggtgcg gtgtttatgg caggcgcatc cattcagtgg ctgcgcgatg
    13801 agatgaagtt gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca
    13861 ccaatggtgt gtatgtggtt ccggcattta ccgggctggg tgcgccgtac tgggacccgt
    13921 atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg
    13981 cgacgctgga gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact
    14041 ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga
    14101 tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg cccggaagtt cgcgaagtga
    14161 ctgcattggg tgcggcctat cttgctggtc tggcggtcgg tttctggcag aacctcgacg
    14221 agctgcaaga gaaagcggtg attgaacgcg agttccgtcc aggcatcgaa accactgagc
    14281 gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac
    14341 acgacgagta atgtaaatgc cggatgaagc gtttatgccg catccggtag tcctgaaacc
    14401 gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct acacttcgcg
    14461 ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg
    14521 tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg
    14581 cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca
    14641 ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag
    14701 tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca
    14761 tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgct
    14821 tcctcaatgc gccggatatg tatatggaga agctgattgt cgggccggga gccaaaggca
    14881 ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtagcggcg gcgctcggca
    14941 aaccgttgag cgaactgacg gtaacgattc tagctaaacc acgccacgat gccgttatcg
    15001 ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggatggcgac gttgcggcct
    15061 caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt ggtggcgcgc
    15121 cggaaggcgt agtttctgcg gcggtgatcc gcgcattaga tggcgacatg aacggtcgtc
    15181 tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg
    15241 agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgc ctgggcgata
    15301 tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg
    15361 aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc cgcggcaagt
    15421 cacgcaccat tcgccgcatt cagtccatcc actatctgga tcgcaaagac ccggaaatgc
    15481 aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg ggactggaat
    15541 ttttttgttc ggagaacgaa gataaggcaa gttaatcaaa acaggagaaa aacatggctg
    15601 attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca
    15661 ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt caccaagctt ggccttgaaa
    15721 tcgacggcga acgcgtccag cgcgcctact cctatgttaa ctcgcccgat aatcccgatc
    15781 tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgattg gcggcactga
    15841 aaccaggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt ctggatgaag
    15901 taccggactg cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat
    15961 cgattctgca actaggcaaa gatttagatc gcttcaaaaa tctggtcctg gttcacgccg
    16021 cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa aaacgctacg
    16081 aagggaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg tcgctcaccg
    16141 ggcggatacc ggcgttaatt gaaagtgggg aactggaaag cgcgattggc ctgccgatga
    16201 ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacacaac
    16261 agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg ggccatatga
    16321 cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga gcaaatccgc
    16381 actcatctta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa atgagtgatg
    16441 atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca gtgtttccgg
    16501 catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt tcgtcagttt
    16561 atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg ttgcagggaa
    16621 gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg tcaggtgagc
    16681 gtcattatgt ctggcgagct ccagagcttt attcaccagt aaggcatctt cttcattccc
    16741 ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa gcaactccat
    16801 caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca tgagaaagtg
    16861 aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttataggc cacgctggcc
    16921 ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat aaaggactct
    16981 gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc gttatcaatc
    17041 accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac atctttcata
    17101 gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg cgccgttaag
    17161 ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt gcttaaggtg
    17221 aagagttcag aaggattttc cgggcattgt catttgcatc gtggaaataa caatcccgga
    17281 aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt cggctttata
    17341 cttcacatcc tgggtatctt taccatattt attttcgcct tgtgtgccaa tgaacgctcc
    17401 aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacgcccca ccgcccattg
    17461 ccagacaccc ggtaaaatcg cccagttacc cgccagcagc atccacgcca caatcatcag
    17521 aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta ctgccgctgt
    17581 tggccaaagc aagcacacca ggcaaaacgc cgcggtctga atatcgagta aatttttacc
    17641 cgccagtgag aaaagcacca gcatgcctgc taaccacagg cctatccaaa tccagaaatc
    17701 acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca tgtaagttcc
    17761 ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat atcgttttaa
    17821 tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc tgttttttct
    17881 cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag atacggcaac
    17941 gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca gccagtttcg
    18001 agaagacttt aacagccaga atcctagcct gccactgaac gaatttcgtg ccatcgacag
    18061 cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa attaacgaga acttgtatgc
    18121 ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga cctggctacc
    18181 catccagggg ccagagcaaa aagcagcgaa agcgaaagct caggaataca tggcagcagt
    18241 gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac tgcagtcgct
    18301 actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac tgcgttatgt
    18361 tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta agctggcgct
    18421 atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt gccgatgaat
    18481 ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt tattcgctta
    18541 taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa actgaacggc
    18601 agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc aggtgttgct
    18661 ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa gcgcgaagct
    18721 gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc cggcgcattc
    18781 accggtgaaa cttctgccgc tatgctgaaa gacatcggcg ctcagtacat catcatcggc
    18841 cactctgaac gtcgtactta ccacaaagag tctgacgaac tgatcgcgaa aaaattcgcg
    18901 gtgctgaaag agcagggcct gactccggtt ctgtgcatcg gtgaaaccga agctgaaaac
    18961 gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact gaaaactcag
    19021 ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc aatcggtact
    19081 ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg tgaccacatc
    19141 gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg ctctgtaaac
    19201 gcgtctaacg ctgcagaact gtttgctcag ccagatatcg acggcgcgct ggttggcggt
    19261 gcttctctga aagctgacgc tttcgcagtg atcgttaaag ctgcagaagc ggctaaacag
    19321 gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta actcttcgcc
    19381 ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac ggttaagcgt
    19441 cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc gtaccatcgc
    19501 cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca gcatcataaa
    19561 tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt catgcccgcg
    19621 caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt tatccagctg
    19681 ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt gcgtacgccc
    19741 ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc cgtatttttt
    19801 gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg aaggatcggt
    19861 caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat attgcagtgg
    19921 accattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg cacatggcga
    19981 aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct tacgtaatgt
    20041 atccgactct tcaccggtta atttccagta accaataccg gcagccacaa cggcgataac
    20101 tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt acctcaaaga
    20161 gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga aaccaccggg
    20221 tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta ccgccgttag
    20281 caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg gtgaataact
    20341 tcagctttgg aaacgcattt tcgtactttt tcgctacttc agcgtcgcgc ggacggtagt
    20401 agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg taggcttccg
    20461 ccacctcttt ggtgcctttt ttctcgacca ctttatcgac caccgacacg gttggctctg
    20521 cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt tcattcgctg
    20581 ccagcagagc ttcgttttcc caagcgatca gtacatcgcc aatcccgcgc tcgacaaagg
    20641 tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac agtgcccgaa
    20701 caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag ccccaggcag
    20761 ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt aggcgtgatc accgaaacac
    20821 ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attgccctta cgcaccagga
    20881 aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc cactctttat
    20941 caatccgccc gcgttccgca atcgcgtcca cgtcataggc cagagccagc gtgacaacat
    21001 cagcttcaat accgttgatt accgacgtcg cttgtttacc tgaaccaccg tgtgactgac
    21061 gaatcaccac gttatcacca gtttgctgtt tccagtgggc gctgaatgcc ttgttgtact
    21121 gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata tcctttgcca
    21181 taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc atcgcccgac
    21241 tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa cattaaagaa
    21301 taaaaaaaga ttggctataa cttgcgggta tatgttgagg gattaaaaag gcggaagaaa
    21361 tccgcctcat attgctgaca aagtgcgctt tatcgatgcc ggatgcggcg cgaacgcctt
    21421 atccgaccta caaaaatttg taaattcaat aaattgcaga attcacgtag gcctgataag
    21481 cgaagcgcat caggcatttt tgcttctgtc atcggtttca ggctaaagga atctgccttt
    21541 ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcaccttt gaacggacgc
    21601 ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctggatacct
    21661 acacaacgac cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc catacgggaa
    21721 gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt
    21781 gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac
    21841 atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt
    21901 tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa ttcacagcca
    21961 ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac ttccaccacg
    22021 gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg
    22081 ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg
    22141 cccggcagac cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa
    22201 ccgtcaccgc cgataaccac cagcgcgtcg atcccacgtt ttttcaggtt ttcgatagcc
    22261 acggcgcgga tgttctcgtc gcggaattcc gggaagcgcg cagaaccgag gaacgtaccg
    22321 ccacggttga tcatgtcaga aacgctgtaa cggtctagct gtaccatacg gtcttcatac
    22381 agacccagat agccgtcata aatgcccatt acttccagac cttctgtcag cgcagaacga
    22441 acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacaccg
    22501 attttcttaa tcatgactac ctctgaactt tggaatgcaa aataaaatct gttgccggaa
    22561 gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg
    22621 ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct
    22681 ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac gacggaacag
    22741 gggtcctgat ggataattac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc
    22801 tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc
    22861 tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc
    22921 ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa tgcgcgatcc
    22981 agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg
    23041 attcccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa ccccagcgcc
    23101 agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc
    23161 gcctggcttt gcgtccggcg caccacccaa cgctgaaacg agacaaggat aatcgtacaa
    23221 attagcgcca caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat
    23281 atcagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat aaacatactt
    23341 tgcgccagcg ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt atcgtcggca
    23401 ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat atccaccagc
    23461 gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta
    23521 atcagcaata gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt
    23581 ccataagatt gattcataaa tactcccgct atcaactgac gctagtataa cggaagcaaa
    23641 tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt
    23701 gggggaagac agggatggtg tcacaaaaaa gcacctaacc tgatgatata aatggattta
    23761 tgtcaaccgc tgtccacata gcgtccacat cgttcagcgt ccgtttaatc ggacgctttt
    23821 aaatttttat ttttcccagc agaaaaatta gctttcccgc tgttttgcaa ctcaacaaga
    23881 ggagataacc gtcggtaaac agccaaaagt atctttaact gaaccagttc atctgccccc
    23941 gatagttgaa ccaagttttt acgttgttct tcactaagtg ccatcacaga ttcaagtgat
    24001 ttaactgcgt cagaaacaag ttcaatctct ttaatttgat tctgtatacg cagcttctct
    24061 gatttcaact ttgcccattc tctttgtcga tatgtacgca tcacctgatc gtaaactttg
    24121 ttggcagcca gaatacttcc accaacagca gtaactaact cagcaatctg ccaagagata
    24181 tcaataagag cagacaattc aataaagcca ggagaggcat actgaatttt tttcactaca
    24241 ggacgtaaat ctggtggagt cgcactgtaa gcccctctaa aaaagttcac cacactgtgc
    24301 cccccctccc aaggcattgt tgaatatcct cttgatcctc cttttaggcc ataaaaaaaa
    24361 ccatatagct gcaaatactc tttcgttaag accgcgaaat cagctaactc ccaacgatta
    24421 tcaagctcaa ttataacttt ttccattttc caccaacctg tccaacttta aagattgact
    24481 tttaagtata tgataaacat agtttattaa acgtgatcca ctacttttaa tgaagaatat
    24541 tccccaaaac agaaagcccc tcgactgagg ggctttctgt ttgtaattac atccacataa
    24601 tttgctgccc tgacggcaac gggtgcggcc ttacggcgtg gacttctccc ggcttcacaa
    24661 tgtatcgctg taccgactca taagtgatga acgtggcgct gcaattcacg ttctgacact
    24721 ggtgataacg ctcttttgtc gtgtcagtga tatagcgact tgtacgcgca tgtgcggcat
    24781 gctggcataa aggacaatga aacatcgcga gcacctcttc cggttttgtt gatggtgcca
    24841 ttttagttaa tttatcctta taaaacaaat agataaaata aaaacatcac tcatcatctt
    24901 ctgtttcgta ctccacatca gaaagcctga cctcaagctc cagggacgtc gtgaagccgc
    24961 tattattcag aaaatgtgtc accttagtga ttgtccagtc ctgctcgtct atgacgcgct
    25021 tacacgaccc gcgtcgtcag gcggcgctgc tgtactggca ggggttttcc gtgccgcaga
    25081 ttgccgccat gttgcagatg aaacgcccga cggtgcagag ctggaaacag cgcgacggct
    25141 gggacagcgt tgcccccatc agccgtgtcg aaatgagtct ggaagcgcgg ctgacccagc
    25201 tcatcatcaa accgcagaaa accggcggtg acttcaagga aattgacctg ctgggacgcc
    25261 agattgaacg actggcacgg gtaaaccgtt acagtcagac cggcaacgag gcagacctta
    25321 atccgaacgt cgctaaccgc aacaaaggcg gacggcgcaa accgaaaaag aattttttca
    25381 gtgacgaggc catcgaaaag ctggagcaga ttttctttga gcagtctttc gattatcagt
    25441 tgcactggta tcgcgccggg cttgagcacc gcatccgcga tatcctgaaa tcccgccaga
    25501 ttggcgcaac gttttatttt tcccgcgagg cgctgctgcg cgccctgaaa accggtcata
    25561 accagatttt tctgtcggcc agtaaaacgc aggcgtatgt gttccgcgaa tacatcatcg
    25621 cctttgcccg gctggttgac gttgacctga ccggtgaccc gattgtcctg ggcaataacg
    25681 gcgcaaaact gatttttctc ggcaccaact ccaacaccgc gcagagtcat aacggcgacc
    25741 tgtatgtcga cgagattttc tggatcccga attttcaggt actgcgtaag gtggcatcag
    25801 gtatggcctc acagagtcac ctgcgctcga cctatttctc caccccgtcc acgctggcgc
    25861 acgacgccta cccgttctgg tcgggtgaac tgtttaaccg gggacgcgcc agcgccgctg
    25921 aacgcgtgga aatcgacgtc agtcataacg cccttgccgg aggtcttctc tgtgcggacg
    25981 gccagtggcg gcagattgtc accattgagg acgccctgaa aggtggctgc acattgttcg
    26041 acattgagca gcttaaacgc gaaaacagcg ccgacgattt taaaaacctg ttcatgtgtg
    26101 aatttgttga tgacaaggca tcggtattcc cgttcgagga gctgcaacgc tgcatggtcg
    26161 acacgctgga agaatgggaa gactatgcgc cgtttgccgc aaatccgttc ggctcccgac
    26221 cggtatggat tggttacgac ccgtcacacc gtggcgacag cgccggatgc gtggtactgg
    26281 caccgccggt ggtggccggt ggcaaattca gaatacttga gcgtcaccag tggaaaggta
    26341 tggactttgc cacccaggcg gaatccatcc gcaaactcac cgaaaaatac aacgtcgaat
    26401 acatcggtat tgatgccacc ggcctcggtg tcggcgtgtt ccagcttgtg cgctcgttct
    26461 atcccgccgc gcgcgacatc cgctacacgc cggaaatgaa aaccgcaatg gtgctcaagg
    26521 caaaagacgt tatccgccgt ggctgtctgg aatatgacgt cagcgccacc gacatcacca
    26581 gctcgttcat ggctatccgc aagaccatga ccagcagcgg acgcagcgcc acctatgagg
    26641 ccagccgcag cgaggaagcc agccacgccg acctcgcctg ggcgaccatg cacgccctgt
    26701 taaatgagcc actcaccgcc ggtatcagca ctccgctgac atccaccatt ctggagtttt
    26761 actgatgagc aagaaaaaag ggaaaacacc gcaacctgcg gcaaaaacaa tgaccgccag
    26821 cgccccgaaa atggaggcat tcacctttgg tgagccggtg ccggtactcg accgccgtga
    26881 cattctggat tacgtcgaat gcatcagtaa cggcagatgg tatgagccac cagtcagctt
    26941 taccggtctg gcaaaaagcc tgcgtgctgc cgtgcatcac agctccccga tttacgtcaa
    27001 acgtaatatt ctggcttcaa cgtttatccc gcacccgtgg ctttcccagc aggatttcag
    27061 ccgttttgtg ctggattttc tggtgttcgg taatgcgttt ctggaaaagc gttacagcac
    27121 caccggtaag gtcatcagac tggaaacctc accggcaaaa tatacccgcc gtggcgtgga
    27181 ggaggatgtt tactggtggg tgccgtcctt caacgagccg acagccttca cgcccggctc
    27241 cgtgtttcac ctgctggagc cggatattaa tcaggagctg tacggcctgc cggaatatct
    27301 cagcgccctt aactctgcct ggctgaatga gtcggccacg ctgttccgcc gcaagtatta
    27361 cgaaaacggc gcacatgccg gatacatcat gtacgtcacc gatgccgtgc aggaccgcaa
    27421 cgatatcgaa atgcttcgcg aaaacatggt taagtcgaaa ggccgcaaca actttaaaaa
    27481 cctgtttctc tatgccccgc aggggaaagc tgacggcatt aaaattatcc cgctcagtga
    27541 agtggcaacg aaggacgatt tttttaatat caaaaaagcc agtgccgcag acctgctgga
    27601 tgcgcaccgc atcccctttc agttgatggg cggcaagccg gagaacgtcg ggtcgttggg
    27661 tgatattgag aaagtggcaa aggtctttgt ccgcaatgag cttatcccgt tacaggacag
    27721 gatccgcgag ataaacggct ggctcggtca ggaggtcatc cgctttaaaa actactcact
    27781 ggacactgac aacggctaaa catcgccgcc tgcgggcggc ttttttacac cccgtcatca
    27841 cgccctcaca cactcaccac cgcacaaaac agcccgcaga cacaccaacg ccccggtgca
    27901 caatctaaac gccatcaaga cgcgctcaga cgctgaaaaa ataaaatcag caccaccgcc
    27961 agcgcgcagt gctttccccg cctcgcccgc ccgcttcacg gggcggtttt aatgcagttg
    28021 catagatact atggatccgc accagtcctg accgcacgca gcctgaacgg acatccccaa
    28081 cgcatgcaaa aacattcact tgttgcatgc atagcttttt aagtacgcca taccgcaact
    28141 gtacattttt aagcaattgg caactttaaa aaatttacat tgctttcaag accttatcat
    28201 ccgtagtctc tgttttttac tttgagctac atcaataaaa tctcaaacat gtttaatgca
    28261 aagcccttgt tacacaacat agaatgtatg tctagaaaca acgacatact atatgttgtg
    28321 tttttccggc tctctgttcg gtgatatgcc aaatcacttt gcgtttaaac agagcattat
    28381 ttcagggcaa gacttcgctc aaaagtcatt ccacctaaga agcgcatata ccggtggaag
    28441 ttgccctcta cttacaggag gcaatatgaa gaaatgctat tactgcattc tcgttctggc
    28501 cctctttggc tatccaaacg gtagtccgag tggtttgtca gtaaacgtca gtaatatcaa
    28561 tgtcagcatt atgctttaat atgcttcaaa caaaaaaacc acctgccagg gtggtttttt
    28621 tgcgcccatc atcaatacga tgaaagactg acaagacttc gctcaagacg aattatgcgt
    28681 cgacataagg ttacccgcaa tgattttatt agactaaatg gatggccttt tatgacccgg
    28741 atcaaacggc tgtccttacc gacatgctag tcactcaata gcatttagcc ttctaattca
    28801 gcatgctatt gaccttctgc tgccccataa ctgctccgca taagccattc aatgccatat
    28861 caaatcacgt tgtgttttta ctcaaatggg taacgagaac cccggccact catcagcaac
    28921 cggatacgtg aattttttcc cgtcataatt tacggtcgcg ccacgcgcca gcgcctcaag
    28981 ctcccatcgt tgcggcctga taccgttctg agcgaggtca acgcggatac gggtaatttg
    29041 caatcgttca gacctggtca gtctggcaga tggtgcaatt tcatgcggtt ttaacgggat
    29101 tccgtttctt tgctgacgat ttggtgttct cagtccgtgt tttaatgcgc ccctgagcgc
    29161 catcacgacc tccgggtcac tccattcgat aacaccgtca tctaccagat taagcactgc
    29221 tgcggcgtgc tcagaaggtg tgggagccgg taacgaagta tcaccaccgg tgagctttcc
    29281 acagttattg acaggactcc gaggcgcggc gatgccgctt tttaaagtca aaggctcaac
    29341 gaccggcact ttcggaacaa tgcgccagtc cgtcgttctg gtgatatgaa tatgacgcgc
    29401 gccgagatgc ggcgcgtaaa tgccgaccac tctctcgact tcttcctcgt actcgttaac
    29461 gtcatccgac ggactacggg cgaccctgac agtctgacaa tcgcgcggga catttgcccc
    29521 accctgcgcg ctgatataca acgcaaaatc accactgtct gcggcagcgc gagcagcctc
    29581 gacgcgttcg tcaaactcat cagcaatact gacgccgcga ggcaatttgc gtagttcacg
    29641 gtaagccccc attgtcggca gtccaaccgt tttaaattgc gggatgcgcc acgttgacgc
    29701 ccatgcggta acagccgcgg cagtatcttt aagcggctta ccggtatcgt tatcgagctg
    29761 accatccagt gcatagccgt cgatattttt tgaaatgtat ttcgcgatat atcccgcagc
    29821 accgccccgg ttaaggtgct ttgcctgaaa acggtttcgc gcggctcctc tttcgtcacc
    29881 atcctctttg agcgcatagc gacgcatgat ttcgataatc tggttacgct ggcgtggatt
    29941 acaaaaaagc atcatatgcc agtgcggcgt tccgtcgtgg tgtggctcga cgacacgcaa
    30001 accgtagacc tgtaaatcat tatccttgaa tgccgtgcgc atcaggctcc agatgcggca
    30061 gagataacgc tgcgcatcct ttggattaaa tgcctcatcg ttccagccgt gattaagctg
    30121 aacggtttta ctttcgcctt ttctgacctg acgagtcggg tgatactttg acggcgcggt
    30181 cagcgtgata aacatcccca catcaccctc tgcggcggcg taacgctcaa taccggcaat
    30241 ggtgttcatc agctccatcc ggcgaatttc aggattagaa atactgccca tcaccttact
    30301 gataaggtcg atgcgctcgc cggtttccct gttttcaagg tcacacgatt taagaaattc
    30361 cagatttgcc tggcggcgcg cacgcacatc acgaatggcg tgtttactgg cataaggaga
    30421 acggtcttta ttgacctccc cgacagctat cagtaacgct tcatgccagc gcatacgctg
    30481 gcctttaagc tgatgagtcc accactcatc gttaaacaga cgggcaatgg cagaatatgc
    30541 ctgcctcgtg gtcatctgcc ctttacggta ttttttccag tagagcgggg aaatattgaa
    30601 agcacgtgca gcgccagcaa catgaccata gaggtgagcc tgcgcctcat ccgtaaacag
    30661 cgattctttt tcgccatgcg catccaccca ggcatcgctg agttcctcat acatcatgaa
    30721 aagctgcgat gagatacggg cagcaaactt tttcagctcc ttgtcattca ttcccggcag
    30781 gcgcgcatag tggtcacgct ctgccagaaa cagtaacgac gcgtcggtgt tcatttcatg
    30841 gcgctgattc acacgctcaa tgcgcggcca taaacgacgc tgaaaagtgg atgtgaggaa
    30901 ataaaacccg tgcaccgggc ttttattgcg ccggatgtag tcatagcgtg aagtaaacag
    30961 cgagcgcaaa aagtaaggca ggcggttaat cgtggataaa acaccttgca cctgacgcat
    31021 ctcgtcacgt gtaaggggtc tttcgcgccc gacggcctcg cgtggcgcgt tccatgcata
    31081 agcaccggta aacgccttac cggtgcctgc agcaaatgct gacggaggga caaaacgccc
    31141 ggaggcttta acggccatat gagccaaaag cctctgaaca acgcctgctg agttgctcaa
    31201 cctgcgcgtt taaatcagca aaagactttg cgcttccggt cagaatatcg tgatgcatca
    31261 ggctggaaac gagctggctt aatttcggat aataaccaac caccgccagc cattcctgac
    31321 cggcgttttt accgctttcc gctctctttt tctcgtggag aataaactga aagctgtcac
    31381 tggtaacgac ataacgttcg ccaatttcaa tacgaatact catgccgttc tccggtaatg
    31441 tttgtttttt gcttcaaaga ctgactggca ggaaacacaa cgcgtggctg acggatatcc
    31501 ggcatgttca caaaattaat tcaattggat gaattttccg ggcaacaccc aattcacgga
    31561 ctgccgccac tggcggaggc caaccctgaa atgacgttcc tggaaaaagc agaaatcgaa
    31621 aaactgttaa atgttttggc tggtgatgac ttacttgtcg cgcttttatg tctgagcact
    31681 ggaggaagat ggacggaagt tgccacgcta aaaccagcac agattacaaa ttgcagggtt
    31741 accttcctga aaaccaaaaa cggtaaaaag cgaaccgtgc cgatttctga ggaactggag
    31801 aaaaaagtta aagaggaggc cagcgccaaa ttattcaaag ttgattatga gaaattttgc
    31861 gggattttac gcagagtgaa acctgatata ccacccaatc aggcaaccca catcctgcgg
    31921 catacattcg caagccattt catgatgaat gggggcaata taatcgcact gcaacagatt
    31981 ctgggacatg cgagcattca gcagacgatg gcctatgcgc accttgcgcc tgactatctg
    32041 caaaatgccg tcgctctgaa tccactaaaa ggcggagtga cgttataaat ttccccctct
    32101 gagtgtccac atagtgtcca cactcaagaa attttgaaac cgctccaaac ccttacagct
    32161 ttcgataagt acctgttttt acttttgatt gcatgtaagt aattgataaa aaaaaccccc
    32221 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    32281 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    32341 gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    32401 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    32461 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    32521 gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    32581 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    32641 catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga
    32701 accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca tgacggcagc
    32761 ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac gatagagagt
    32821 ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta
    32881 gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat aaaatcctgt
    32941 tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag atggaaggct
    33001 tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac gacagcattg
    33061 atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca ttaaaagcac
    33121 ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt gaacttgatc
    33181 gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt aatgatcgtg
    33241 agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgag caacagcaaa
    33301 acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat ccaggccgtc
    33361 aggaagccag cttcgacgga caaacgctgg agttaaccgg cactgagttt accctgctct
    33421 atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc caggaagtgc
    33481 tgggcaaacg cctgacgcct ttcgaccgcg ctatcgatat gcacatttcc aacctgcgtc
    33541 gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct
    33601 atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg ccatcttctg
    33661 gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg attcacgcca
    33721 gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc agcatgtcga
    33781 agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc
    33841 gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg aaggccgcgt
    33901 gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc aggccgataa
    33961 cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt
    34021 gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca gttctcaatc
    34081 cgatttcatt aacttactgt ttgaccgccc gctattactg ctgattgtca ccatgttggt
    34141 cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc gtaagctgaa
    34201 aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac tggaagcggg
    34261 gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg cgctggagcg
    34321 catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc gcaccccgct
    34381 gacgcgtctg caactgggta cggcgttact gcgccgtcgt agtggtgaaa gcaaggaact
    34441 ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc tgttggtgat
    34501 gtcccgtaat cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca accagttgtg
    34561 gagtgaagtg ctggataacg cggcgttcga agccgagcag atgggcaagt cgttgacggt
    34621 taacttcccg cctgggccgt ggccgctgta cggcaatccg aacgcactgg aaagtgcgct
    34681 ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag tgggctttgc
    34741 ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg ttagcccgga
    34801 agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg atcgtgaatc
    34861 tggcggtaca ggattggggc tggcgattgt tgaaaccgcc attcagcagc atcgtggctg
    34921 ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt tgccgctgta
    34981 taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg catccggcat
    35041 ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac tccgggggtt
    35101 attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca
    35161 tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga tattgatcgt
    35221 catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca tcgctgacac
    35281 gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc acgctataca
    35341 accaccccac cttacctgtg ttttgcatca actgcgctat atcgctgata tcaaaatggt
    35401 aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc tctccccagc
    35461 ggaaaatatc gcccatataa acattacttt ccgtgagacc gtcggttgag agattttcgc
    35521 caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcgcc cagtagagat
    35581 aatgctcacg aggataatga cacagcgctc tgtctggccc gccgtgaacc tttttctccg
    35641 cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca acctggattt
    35701 tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg tatacatcaa
    35761 ccggatatcg catctgttcc ccctccacga gaaatttccc cattagacca tatttatttt
    35821 atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag caaaccggac
    35881 gaacgttagt tcatccggct gtggggatta aggaagcagg tttatttcac cgtacctaaa
    35941 atttcgattt tcgctgctct ggctttgact ttacgtgacc agatagaggt aagaattggc
    36001 accagaatcg aagtcacaat gaccgccgtc gctaccagcg aagttgctgc cggagccatc
    36061 ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc ggcccctgcg
    36121 gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt atcggcgata
    36181 atcaacggaa taccggtcac gataattact gccacaccca acaggatccc cagcaaacca
    36241 gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg cgaaggcaaa gaatggaatc
    36301 agcgtttgca ccgctttgct gaaaaattct cgtaattcag ggtcaaggtt ccccagggca
    36361 aagcccacca ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc
    36421 cctgcagtgc ccagaataat catcgtcatg agcggcccgg actccaacga catcaacaca
    36481 aatgccccag cttcttcttt tgtgccgtac tgctgcatga tggaagcgta aagtccgcca
    36541 ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa gaatccaact
    36601 tcaacaccat gttccggaat aatgcgcgag gcaattgccg caaccaccca cgcgacggca
    36661 atttttgtta ccaccagcgt accggattta cgcaataccg ttcccgtcgc gcttaatttt
    36721 attgacgccc ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc
    36781 ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg gcacagtgcg
    36841 ccaaggaata gcgggacgag catcatcccc cccgggattt tctcaatcga gcgttttatc
    36901 tgcatttcca tcacctgtcg gcatattttt aagggctgat gcgtcattcc tcaatggagc
    36961 atcagtgttg atggaaacga agatagacat tcatttaatc aataaaagtg atcaaaacca
    37021 catcaattga aacactgttt tatttttatc ggatcattgt tttcattttg aaattgatca
    37081 caaaaaaacc accgccgttg gcgatggttc attttttaag ctgatatgcg gcctatacgc
    37141 ctcattgcag caggcggcaa atgattattt tttcgccgca aaacgtgccg ctgcttcgtc
    37201 ccagttcacc acgttccaga actctttaat gtagtccgga cggcggttct ggaatttcag
    37261 gtagtaagca tgttcccaca catccaggcc cagaatcggg aagccggaag cgccagaaat
    37321 agcttcaccc atcagcggag aatcctggtt agcagtagaa accaccgcca gtttatcgcc
    37381 tttcagcacc agccatgccc agccggaacc aaagcgggaa gctgccgctt tttcaaattc
    37441 tgctttgaag ttatcaacgg agccgaagtc acgttcgata gccgctttca ggtcaccctg
    37501 cagggtggtg ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc
    37561 gttgttgcgc agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcagctc
    37621 ttcaaccggc aggttggcaa attctggcag gctttccagc gccgcgttgg cgttgtttac
    37681 gtaggtctga tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc
    37741 cagggcatcg taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat
    37801 tgtcgggcgg ccgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg
    37861 atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg
    37921 cccgagatgt gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc
    37981 ggcaacaggc gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg
    38041 aagcgacgaa aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt
    38101 ggcatttgat cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa
    38161 aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt
    38221 gggccatcag cgccctgtta ctaccgaatt tctggacgta ttacagctcg tttagtctct
    38281 ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactatg
    38341 gcctgaccat gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt
    38401 tgattgtcgg tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ctgattaaca
    38461 ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc gctgattggc gtagggattg
    38521 taactcgagc cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc
    38581 tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg
    38641 cgataaacgc cgcaaaaccg atgcatgaag ccgctgccgc actaggcgtc gatccactgt
    38701 atgtcgctct gccaagctat gtcatcatca tgggcggcgg cgcgatcatc aacctcggtt
    38761 tctgttttat tcgtctggca aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg
    38821 caaaaccgct gatcactcac aatgtgttac tctcagcact gggcgggttg atgtggtatc
    38881 tgcaattctt tttctatgcc tggggccacg cccgcattcc ggcgcaatat gactacatca
    38941 gttggatgct gcatatgagc ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga
    39001 aagagtggaa caatgcagga cgccgtccgg tagggctgcc cattaaacac atgaataccc
    39061 gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc
    39121 tgcgggagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta
    39181 tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg
    39241 tcaggttctt accttaaatt ttcgacggga aaccacgtaa aaaacgtcga tttttcaaga
    39301 tacagcatga attttcagga aatgcggtga gcatcacacc accacaattc agcaatttgt
    39361 gaacatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg tcagtagcga
    39421 gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat gaaattcagc aggatcacat
    39481 tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct
    39541 ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc gttttaaaaa
    39601 tgggctgcat agccagaacg gttatgtcac ctggaatgtg gatagcctgg aaagtgccat
    39661 tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca ttgggattga
    39721 tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg gcctgcccgt
    39781 tgcttatcgc gatagccgct ccaatggcct aatggcgcag gcacagcaac aactcggcaa
    39841 acgcgatatt tatcaacgta gcggcatcca gtttctgccc ttcaatacgc tttatcaact
    39901 gcgtgcgctg acggagcaac aacctgaact tattccacac attgctcacg ctctgctgat
    39961 gccggattac ttcagctatc gcctgaccgg caagatgaac tgggagtaca ccaatgccac
    40021 caccacacaa ctggtcaata tcaatagcga cgactgggac gagtcgctac tggcgtggag
    40081 cggggccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca ttgggcactg
    40141 gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg ataccgccag
    40201 cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt ctggcacctg
    40261 gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc tggcagccaa
    40321 catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt
    40381 atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac gatctcccgg cgcttatcgc
    40441 cgcgacacag gcacttccgg cctgtcgctt catcatcaat cccaatgacg atcgctttat
    40501 taatcctgaa gcgatgtgca gcgaaattca ggctgcgtgt cgggaaacgg cgcaaccgat
    40561 cccggaaagt gatgctgaac tggcgcgctg tattttcgac agtctggcgc tgctgtatgc
    40621 cgatgtgttg catgagctgg cgcagctgcg cggtgaagat ttctcgcaac tgcatattgt
    40681 cggcggcggc tgccagaaca cgctgctcaa ccagctatgc gccgatgcct gcggtattcg
    40741 ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat atcggcatcc agttaatgac
    40801 gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct
    40861 gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc agattcactc
    40921 tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc ctgggagctg
    40981 gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg ccaacttgat
    41041 cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt tgaaaacccg
    41101 gaaggttcgc tgaccggtgg gattcaagct actggtaatt atccgggcaa agcgcgtaat
    41161 gccagtgagc tacgtaccga tctggaacag gctatgcggc tgattccggg gccgaaacgg
    41221 cttaatttac atgccatcta tctggaatca gatacgccag tctcgcgcga tcagatcaaa
    41281 ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg tctggatttt
    41341 aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctttc ccatgccgac
    41401 gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt ttcggcctat
    41461 tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga tggtatgaaa
    41521 gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact ggatgaggtg
    41581 atcagcgaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa attgtttggc
    41641 attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta tgccaccagc
    41701 cgccagactg cgctgtgcct ggacgccggg cacttccacc cgactgaagt gatttccgac
    41761 aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt
    41821 cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa cccaggcaat tgccagtgag
    41881 attgtgcgtc acgatctgtt tgaccgggtg catatcggcc ttgacttctt cgatgcctct
    41941 atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt
    42001 gcgttgctgg aacctaccgc cgagctgcgc aagttggaag cggcgggcga ttacactgcg
    42061 cgtctggcac tgctggaaga gcagaaatcg ttgtcgtggc aggcggtctg ggaaatgtat
    42121 tgccaacgtc acgatacccc aacgggtagc gaatggctgg agagcgtgcg ggcttatgag
    42181 aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcacgagc gccttatccg
    42241 gcctacgggt cgaatgtcca tgcgggctgt caccctcacc ccggccctct ccctgaaagg
    42301 gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacactgctc cggacagtcc
    42361 cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat gtctaaacaa
    42421 ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct ggtttgtcca
    42481 gggaatgatc aaagccacca ccgatgcctg gctgaaaggc tgggacgagc gcaacggcgg
    42541 caacctgacg ctgcgcctgg atgacgccga tatcgcgcct tataaagaca acttccatgc
    42601 acaaccgcgc tatatcccgc tcagccagcc catgccttta ctggcaaata caccgtttat
    42661 tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt gatcctgcag ctaacttagg
    42721 cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt ctctgggggt taaccaacga
    42781 agccgttcca acttctgaac ttccggctca ctttctctcc cactgcgagc gcattaaagc
    42841 caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga tcgccctcac
    42901 ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag gcagcaccga
    42961 gtgtctggtg gtattcccgg atggcgttgg cattctgccg tggatggtgc ccggcacgga
    43021 cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt tgtggccctt
    43081 ccacggcgtc ttcggcagcg ggtcgacgtt ggatgaaaca ttcggtttaa tcgacaccgc
    43141 agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga aacagaccat
    43201 cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac tagccagtgc
    43261 gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg ttaaccccga
    43321 cgcccacgaa gagtatcagc gtcggcataa ccccatctgg ccggaactgg aagcagtgtt
    43381 gaaatctcac ggtgcgcata actacgccat ttacctcgac aaagcgcgta atctgctgtt
    43441 tgccacggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta ctgatgtttg
    43501 ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccggata acagcccggt
    43561 gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc cggagatgcc
    43621 tccggcacaa tattttaaat ttcgatttga aaaattaaac atattaca
//
LOCUS       DAPHDX010000023        43610 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPHDX010000023 DAPHDX010000000
VERSION     DAPHDX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.023"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1250)
                     /locus_tag="RH058_001213"
     CDS             complement(<1..1250)
                     /locus_tag="RH058_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3136828.1"
                     /translation="MALTEVKVRNAKPTEKPVKLTDGDGMHLLVHPTGSKYWRLQYRF
                     SGKQKMLALGVYPEVSLAEARRRREEARQLIANNVDPGEKRKTEKIEEKGLLIFETVA
                     RDWHSSNRTWSDSHRTTVLNSLITHVFPVIGKRNISELKTRDLLVPLKKAEETGHLEL
                     AARLQQRITAIMRYAVHNALIDQNPAYDLAGAVTTAKSNHRPALPLERLPELLKRIDA
                     YKGKGITQIAVQLTLLTFIRSSELRFARWSEVNFKNSLWTIPAERRELEGVKYSNRGS
                     KMKTPHLVPLSKQAVVLFKRLQAISGEKDLMFIGFSRDDKPISENTVNKALRTMGYDT
                     KAEVCGHGFRTMACSALIESGLWSRDAVERQMSHQERNSVRAAYIHKAEHLEERKLML
                     QWWADYLNANRKEHITPFEFAQRI"
     gene            complement(1515..1715)
                     /gene="torI"
                     /locus_tag="RH058_001214"
     CDS             complement(1515..1715)
                     /gene="torI"
                     /locus_tag="RH058_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HDV3136829.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1773..1943)
                     /locus_tag="RH058_001215"
     CDS             complement(1773..1943)
                     /locus_tag="RH058_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136830.1"
                     /translation="MHFRVTGEWRNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTN
                     IRIEELKEHQAA"
     gene            complement(2016..2300)
                     /locus_tag="RH058_001216"
     CDS             complement(2016..2300)
                     /locus_tag="RH058_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3136831.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMLREYDRL
                     IITKGYPKRDDSSRRIDVPYGGYEVKTITHPHFGDKPVKVFAIKVNISTE"
     gene            complement(2300..2560)
                     /locus_tag="RH058_001217"
     CDS             complement(2300..2560)
                     /locus_tag="RH058_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eaa protein"
                     /protein_id="HDV3136832.1"
                     /translation="MIDVKEKVLQVMRSRAALQEKALGGEYPFTIATWNLRLAMEKEF
                     PDEEWRSADLRKILMELAKDGAVSKDTYASRIGQAVWRLEVR"
     gene            complement(2557..2706)
                     /locus_tag="RH058_001218"
                     /pseudo
     CDS             complement(2557..2706)
                     /locus_tag="RH058_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(2707..2994)
                     /locus_tag="RH058_001219"
                     /pseudo
     CDS             complement(2707..2994)
                     /locus_tag="RH058_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2991..3158)
                     /locus_tag="RH058_001220"
     CDS             complement(2991..3158)
                     /locus_tag="RH058_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDV3136833.1"
                     /translation="MRGLAYNPDILPAEMIIRQRVKPMPSREELLKRNSFPSVNENKY
                     LNAMWRKGGSQ"
     gene            complement(3169..3462)
                     /locus_tag="RH058_001221"
     CDS             complement(3169..3462)
                     /locus_tag="RH058_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDV3136834.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3486..3869)
                     /locus_tag="RH058_001222"
     CDS             complement(3486..3869)
                     /locus_tag="RH058_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136835.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVIFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSPQQQSSNQQNTPVYDDSIPF"
     gene            complement(3869..4474)
                     /locus_tag="RH058_001223"
     CDS             complement(3869..4474)
                     /locus_tag="RH058_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDV3136836.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(4485..4655)
                     /locus_tag="RH058_001224"
     CDS             complement(4485..4655)
                     /locus_tag="RH058_001224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136837.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            complement(4731..4883)
                     /gene="kil"
                     /locus_tag="RH058_001225"
     CDS             complement(4731..4883)
                     /gene="kil"
                     /locus_tag="RH058_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDV3136838.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PLYRRK"
     gene            complement(4868..5002)
                     /locus_tag="RH058_001226"
     CDS             complement(4868..5002)
                     /locus_tag="RH058_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136839.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(5237..5419)
                     /locus_tag="RH058_001227"
     CDS             complement(5237..5419)
                     /locus_tag="RH058_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136840.1"
                     /translation="MGKDENIIVEDVFHQDYGPEDGIPHHWCCKFYRDGFADYEYFST
                     KDEAYDFAFKNGYKPF"
     gene            complement(5621..5791)
                     /locus_tag="RH058_001228"
     CDS             complement(5621..5791)
                     /locus_tag="RH058_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136841.1"
                     /translation="MQWKCLPAKRSYLRGRIPEIKTTDGEVSGESDIHSIMLEKSAKA
                     ESKAPQASHKGR"
     gene            complement(5710..5982)
                     /locus_tag="RH058_001229"
     CDS             complement(5710..5982)
                     /locus_tag="RH058_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136842.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIARKLSG
                     CVRADKAASLGSLRCKKADECSGSVCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(6688..7056)
                     /locus_tag="RH058_001230"
     CDS             complement(6688..7056)
                     /locus_tag="RH058_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136843.1"
                     /translation="MVGTAIASFLGMLAISTIYGLAHAFIAKSLSEKISQAWAHRSAR
                     FMILVIIAIQGISAFILYGSSLYLLYQGATFTPYTSDYGTLYDGSEDISMAWIVFGLS
                     MAVSVVADIIKVILVLTFAD"
     gene            complement(7075..7791)
                     /locus_tag="RH058_001231"
     CDS             complement(7075..7791)
                     /locus_tag="RH058_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3136844.1"
                     /translation="MENKKSLTTEQLEDAKRLKALYESKKKELGITQYSIADELGITQ
                     GAVGHYLNGRNALNVEVASGFARLLQVSIADFSQSIAAKVAEQAESLKSDANVRYAGE
                     YRAGKRYPVLSSIQAGSWCEACEPYTIKDIDVWLESDAHIQGNAFWLKVEGDSMTAPV
                     GLSIPEGTFVLFDTGREAINGSLVIAKLSDSNEATFKKLIIDGGNKYLKGLNPAWPLV
                     PINGNCKIIGVAIETKLRLV"
     gene            7898..8092
                     /locus_tag="RH058_001232"
     CDS             7898..8092
                     /locus_tag="RH058_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDV3136845.1"
                     /translation="MIRMTLADYAKIHGQAKAASDFGVIQCAISKAILAGRNIMVTVK
                     PDGSVIGEEVRPFPSNKKNK"
     gene            8201..8479
                     /locus_tag="RH058_001233"
     CDS             8201..8479
                     /locus_tag="RH058_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDV3136846.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            8514..8675
                     /locus_tag="RH058_001234"
     CDS             8514..8675
                     /locus_tag="RH058_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136847.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            8662..9552
                     /locus_tag="RH058_001235"
     CDS             8662..9552
                     /locus_tag="RH058_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDV3136848.1"
                     /translation="MGVVKLADYRHNPVQHQEASSMGYVSIHRQFMDSRLYKDSQAVH
                     LWLHLILKANHESTVVNTDIGPITVDRGQMITGRPSLVRETFIPDNKVRSLLRTFESK
                     GMLNICSMGKKFSLFTIVKYDDFQAKNCPTVVQRLSNANSSNGAALSGDYPTVVQRLS
                     INNNINNISNTDVLESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRKN
                     QIRTFWRKAGVITRQLDGHGFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGF
                     DFLLNDNTYLKVREGEHDDR"
     gene            9542..10978
                     /locus_tag="RH058_001236"
     CDS             9542..10978
                     /locus_tag="RH058_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV3136849.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11256..11843
                     /locus_tag="RH058_001237"
     CDS             11256..11843
                     /locus_tag="RH058_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3136850.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPINQITQQRLKEVL
                     LYDELTGKFTWKVKKCQRMNAGDIAGHKSSEGYWVIKVDGKLYKAHRLAWLYMNGSLP
                     KSDIDHINLVRDDNRIANLRLATRSQNIQNVNKKANNKSGYKGVSWDKKSRKWRAQIV
                     INKRKVNLGFYDDPKEAHKVYANKADECFGEFARY"
     gene            11845..12273
                     /locus_tag="RH058_001238"
     CDS             11845..12273
                     /locus_tag="RH058_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDV3136851.1"
                     /translation="MKCVKYHLTNESIRYNAIQYLRTCDLDIIVEFKQRNRSLDQNRK
                     LWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTSRMRV
                     GEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12270..12797
                     /locus_tag="RH058_001239"
     CDS             12270..12797
                     /locus_tag="RH058_001239"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDV3136852.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            12794..12976
                     /locus_tag="RH058_001240"
     CDS             12794..12976
                     /locus_tag="RH058_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDV3136853.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRERARKTR"
     gene            12973..13143
                     /locus_tag="RH058_001241"
     CDS             12973..13143
                     /locus_tag="RH058_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDV3136854.1"
                     /translation="MIDPNRSYEQESVERALTCANCSQKLHVLEVHVCEHCCAELMSD
                     PNSSMYEEEDDG"
     gene            13136..13747
                     /locus_tag="RH058_001242"
     CDS             13136..13747
                     /locus_tag="RH058_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDV3136855.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNVFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            13744..13950
                     /locus_tag="RH058_001243"
     CDS             13744..13950
                     /locus_tag="RH058_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HDV3136856.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            13928..14599
                     /locus_tag="RH058_001244"
     CDS             13928..14599
                     /locus_tag="RH058_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDV3136857.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMNKLDAIGFDTQKDLL
                     ISVGDLVDRGAENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGAWA"
     gene            14590..15108
                     /locus_tag="RH058_001245"
     CDS             14590..15108
                     /locus_tag="RH058_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDV3136858.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSMATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLAI"
     gene            15172..15246
                     /locus_tag="RH058_001246"
     tRNA            15172..15246
                     /locus_tag="RH058_001246"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15205..15207,aa:Asn,seq:gtt)
     gene            15252..15327
                     /locus_tag="RH058_001247"
     tRNA            15252..15327
                     /locus_tag="RH058_001247"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15285..15287,aa:Thr,seq:tgt)
     gene            15570..15893
                     /locus_tag="RH058_001248"
     CDS             15570..15893
                     /locus_tag="RH058_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDV3136859.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            15877..16353
                     /locus_tag="RH058_001249"
     CDS             15877..16353
                     /locus_tag="RH058_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDV3136860.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKKAGGVVNEAEI"
     gene            16337..16729
                     /locus_tag="RH058_001250"
     CDS             16337..16729
                     /locus_tag="RH058_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDV3136861.1"
                     /translation="MKLRYRLVIVAFVVTVIGSFIWSAGHYYSKYQHEKERADEAVRN
                     AESATAITRNVLQSLQIINTVIEANQHAKEQIALDASGASADIRVAVANDDCTNRPVP
                     AGAVKRLHEYANGLRAGSGSSITSQPDG"
     gene            16873..17058
                     /locus_tag="RH058_001251"
     CDS             16873..17058
                     /locus_tag="RH058_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136862.1"
                     /translation="MARLNVEVTPPDSEALNGIFAEIERKYARQPLTPKVIDEMQREA
                     TRLVRRMITTKVTFVRD"
     gene            17115..17735
                     /locus_tag="RH058_001252"
     CDS             17115..17735
                     /locus_tag="RH058_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136863.1"
                     /translation="MNRPHPPSHFTMPPDPKPYISIMPASDVGEWLNQHILSDEGDLY
                     NPDHQHLLEADLCFLWASNAFEKKGRSVLGQAEEVAMRAGGWQKARMEQQMYEWFGRV
                     PQFIITLAADYCSQCSDLEFCALIEHELYHICQATDEFGAPKFTQEGQPKLKLRGHDV
                     EEFVGVVRRYGASRDVQEMIDVANQPAEVAHLDIARACGTCMLRLA"
     gene            17767..18240
                     /locus_tag="RH058_001253"
     CDS             17767..18240
                     /locus_tag="RH058_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2280 domain-containing protein"
                     /protein_id="HDV3136864.1"
                     /translation="MAALKSDVKAFIIQSLACYDTPSQVVEAVQKEFGIKITRQQAES
                     HDPTKASGKTLAKKWIEMFHATRERFLTETSDIPIANKSYRLRVLDRMATKTEGMKNF
                     SLTAQLIEQAAKEVGDAYTNKLKVESTGKDGGPIKTETTNLTADQAAEIYRKMMG"
     gene            18243..19865
                     /locus_tag="RH058_001254"
     CDS             18243..19865
                     /locus_tag="RH058_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HDV3136865.1"
                     /translation="MPLPFEFDFRNPDYQMVFEWRMERLQRIRQNPEMLPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERRERLENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPIEFR
                     GSWDEKKHAPYMRVEFPDTGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NEQRNAARLPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            19865..21331
                     /locus_tag="RH058_001255"
     CDS             19865..21331
                     /locus_tag="RH058_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDV3136866.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGTLEAQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRMPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPESPGGNPISNEPTADSAGGRGYRKWSLRWFK"
     gene            21219..21956
                     /locus_tag="RH058_001256"
     CDS             21219..21956
                     /locus_tag="RH058_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDV3136867.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVSGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            21971..23191
                     /locus_tag="RH058_001257"
     CDS             21971..23191
                     /locus_tag="RH058_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDV3136868.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRQNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            23196..23699
                     /locus_tag="RH058_001258"
     CDS             23196..23699
                     /locus_tag="RH058_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136869.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGSGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMATSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            23711..24652
                     /locus_tag="RH058_001259"
     CDS             23711..24652
                     /locus_tag="RH058_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HDV3136870.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLNGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(24663..24881)
                     /locus_tag="RH058_001260"
                     /pseudo
     CDS             complement(24663..24881)
                     /locus_tag="RH058_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24880..25287
                     /locus_tag="RH058_001261"
     CDS             24880..25287
                     /locus_tag="RH058_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HDV3136871.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNHDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            25284..25838
                     /locus_tag="RH058_001262"
     CDS             25284..25838
                     /locus_tag="RH058_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136872.1"
                     /translation="MSFKSGVTTRVDNAQAILDALKSISKKEVLVGIPEADSEREDVP
                     FGNAGIGYLNEYGSPAQNIPPRPHLIPGVKSVEEQTLPQLKTAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGAKAELARRAAGESPGTDLV
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            25825..26214
                     /locus_tag="RH058_001263"
     CDS             25825..26214
                     /locus_tag="RH058_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HDV3136873.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDDDGIGSDSEVKTQF
                     AGVVTVDRSLENRRMQSGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGSGFVQAHCELLPFDGGIPVEQ"
     gene            26189..26752
                     /locus_tag="RH058_001264"
     CDS             26189..26752
                     /locus_tag="RH058_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136874.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLCRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     KVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            26756..27901
                     /locus_tag="RH058_001265"
     CDS             26756..27901
                     /locus_tag="RH058_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HDV3136875.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            27912..28352
                     /locus_tag="RH058_001266"
     CDS             27912..28352
                     /locus_tag="RH058_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HDV3136876.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGSDGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            28356..28808
                     /locus_tag="RH058_001267"
     CDS             28356..28808
                     /locus_tag="RH058_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136877.1"
                     /translation="MEFEIKGVKYRAAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTSAIIHPCLAVVSRRHMDGWV
                     PVFTQGELMFDDIDLLVMLHLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            28986..30974
                     /locus_tag="RH058_001268"
     CDS             28986..30974
                     /locus_tag="RH058_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HDV3136878.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYRASQRTGATVQGIQSIGYAVSQVGGSVDAARTSLESL
                     SRFVRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSGMPYYRANQYAQMLG
                     IDENTLMAMRRGVGDFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILAIGDIIGRLFFRLIEGTSS
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKGSVNELAKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVIIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQYAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKMTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            30974..31561
                     /locus_tag="RH058_001269"
     CDS             30974..31561
                     /locus_tag="RH058_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136879.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVIISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            31561..31863
                     /locus_tag="RH058_001270"
     CDS             31561..31863
                     /locus_tag="RH058_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136880.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDPYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLLVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            31866..32924
                     /locus_tag="RH058_001271"
     CDS             31866..32924
                     /locus_tag="RH058_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136881.1"
                     /translation="MSQNWMRHFELQLVDGNGKGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVAASDVGTVREVDAADVGQSDGR
                     NYGLIFSGEIRYSVTGKDSPVDTYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVYPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHDAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTTAQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAADLYTQSALQR"
     gene            32931..33272
                     /locus_tag="RH058_001272"
     CDS             32931..33272
                     /locus_tag="RH058_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136882.1"
                     /translation="MRGIIFLLAVFSACSVWADGFTVKCGGYTMVANQGELSTINGER
                     VTSQKITELGTNGLKVDMGIMPAKDGNNYGFEYIRRPGTETRFLNVQLLQNSMDAPKI
                     IGSFPCKKVDG"
     gene            33441..34013
                     /locus_tag="RH058_001273"
     CDS             33441..34013
                     /locus_tag="RH058_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136883.1"
                     /translation="MAISYAFALATITQQMNQVQEAVNGAFKPLISNACELPQRLDAE
                     EVFRRCTAIAARAQEIENTAKEGMSHLEAFRNGRIIVDELPEEFLSHLEGLAKACRNA
                     KGHLVDMFSEAERSPMWQGHLQMLRPLKRKYVRALTAVENTATQLAAEVRQSQPFQAE
                     LLSDNVTREEAIELISTSHKMLGANAHKWM"
     gene            34015..34431
                     /locus_tag="RH058_001274"
     CDS             34015..34431
                     /locus_tag="RH058_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HDV3136884.1"
                     /translation="MAKVSITGELRHLAAAHKYAQMLADYISKGSQFWCFGSLGGFER
                     NYDAMAANIRKIHLKLPGDKPWPPEASLSERTCDNFLVYAQHLYIDEHYQILAIISPN
                     AHQQADSMLPRLIKLAEETFIELPPDELEKLKTYDS"
     gene            34474..35226
                     /locus_tag="RH058_001275"
     CDS             34474..35226
                     /locus_tag="RH058_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV3136885.1"
                     /translation="MAVSDQTRSGDLAETFKSERETTKNQIRVALPGIIQSFDPDAVT
                     AVVQPAIRSVEKDNDGNRITKNYPLLVDVPVVFPRGGGCTLTFPIKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGVVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            35226..35579
                     /locus_tag="RH058_001276"
     CDS             35226..35579
                     /locus_tag="RH058_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136886.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            35579..36778
                     /locus_tag="RH058_001277"
     CDS             35579..36778
                     /locus_tag="RH058_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDV3136887.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMATATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQAISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGGNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            36775..37455
                     /locus_tag="RH058_001278"
     CDS             36775..37455
                     /locus_tag="RH058_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HDV3136888.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWLFPDT
                     DISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGVLL"
     gene            37455..38171
                     /locus_tag="RH058_001279"
     CDS             37455..38171
                     /locus_tag="RH058_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136889.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALENLGLGDISATGVATGSMGETGYAIFPMMIGGTRKTLIMQWGVMTSLRSG
                     GTMDINLPIAFPTMVMQAYATHDNVIQTARPTVYAIAPTSRTTITATAMSLRADSVGA
                     AVTRSSDSDLCYGRYLAIGY"
     gene            38173..38607
                     /locus_tag="RH058_001280"
     CDS             38173..38607
                     /locus_tag="RH058_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDV3136890.1"
                     /translation="MNLKYFSPSENAFYAGELKESYIAADSWPSDALEVDESLYLEFI
                     DEPPAGKYRGVDDAGMPCWLEIPPPTAEEMASNAENMRQQLIDEANVYINKRQWPGKA
                     AIGRLKADELERYNLWLDYLDALEVVDTSSAPNINWPDPPDA"
     gene            complement(38579..38755)
                     /locus_tag="RH058_001281"
                     /pseudo
     CDS             complement(38579..38755)
                     /locus_tag="RH058_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            38755..38982
                     /locus_tag="RH058_001282"
                     /pseudo
     CDS             38755..38982
                     /locus_tag="RH058_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(39174..40331)
                     /gene="intS"
                     /locus_tag="RH058_001283"
     CDS             complement(39174..40331)
                     /gene="intS"
                     /locus_tag="RH058_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HDV3136891.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(40493..40567)
                     /locus_tag="RH058_001284"
     tRNA            complement(40493..40567)
                     /locus_tag="RH058_001284"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40532..40534),aa:Arg,seq:cct)
     gene            complement(40643..41575)
                     /locus_tag="RH058_001285"
     CDS             complement(40643..41575)
                     /locus_tag="RH058_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV3136892.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            41869..42624
                     /gene="mlaA"
                     /locus_tag="RH058_001286"
     CDS             41869..42624
                     /gene="mlaA"
                     /locus_tag="RH058_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV3136893.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(42806..43480)
                     /locus_tag="RH058_001287"
     CDS             complement(42806..43480)
                     /locus_tag="RH058_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136894.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLSAPG
                     VNFDDI"
BASE COUNT        11183 a        10349 c        11227 g        10851 t
ORIGIN      
        1 tttattctct gggcgaattc aaacggtgta atgtgttctt ttctgttggc attaaggtag
       61 tcagcccacc attgtaacat gagttttctc tcttcgagat gttccgcttt gtggatatat
      121 gctgcccgaa ccgagttacg ctcttggtgg ctcatttgac gttctacggc gtctcttgac
      181 cataacccag attcgattaa tgcactacaa gccatagttc gaaaaccgtg accacaaact
      241 tcagctttcg tgtcgtaacc catggtgcga agagccttgt taacagtgtt ttcgctgatt
      301 ggtttgtcat cgcgactaaa accaatgaac ataagatcct tttctccact tatagcttgg
      361 agccttttaa ataatactac agcctgctta cttagcggca caagatgagg ggttttcatt
      421 tttgagcccc tatttgaata cttaaccccc tccaattctc gtctttctgc agggattgtc
      481 cataaggagt ttttgaaatt cacctcagac caacgagcga atctaagttc acttgaacgg
      541 atgaaagtca gtagagttaa ttgaactgct atttgagtga tacctttccc tttataggca
      601 tcaatccgtt tgagaagctc aggaagtcgt tcgaggggaa gggcagggcg gtgattactc
      661 ttggcagtgg ttacagcgcc tgcgaggtcg taagctgggt tttggtcaat caaagcattg
      721 tgaacagcat accgcatgat ggcagtgatt ctttgttgta aacgggcagc aagttcaaga
      781 tgtccagttt cctctgcttt tttcagagga acaagtaaat cacgagtttt aagctcacta
      841 atgttccgct taccgataac aggaaataca tgagtgatca ggctgtttaa tacggtagtt
      901 ctgtgactgt ctgaccaggt tctgttgctt gagtgccagt ctctggcaac agtttcaaaa
      961 atgagtaagc ctttctcttc aatcttttct gtttttcgct tttcgccagg gtcaacattg
     1021 ttggcaatga gttgccttgc ttcttctctt ctacgacgag cttctgctaa agacacttcg
     1081 ggataaacac caagcgccaa cattttttgc ttaccagaaa agcgatattg aagccgccag
     1141 tattttgaac ccgtagggtg aaccaggaga tgcataccat ccccatctgt taacttgacg
     1201 ggcttttcag taggtttcgc atttctgact tttacctcag ttagtgccat tgtgacctcc
     1261 tgatgatggt atctgcatta tcgaacttac aataccaaca gttataccaa caaaacaagc
     1321 tggatgtccg tatatcccaa cagtccttgg tagaccatgg ttaaagtgta cttcattgaa
     1381 atgattggga aaaatagact ttgatatact tcggtagaca gggttctggc gtcccctgca
     1441 gacatctact tgaagcggca ggggattgat tggaatgata ttttttagat gtgagaaata
     1501 ttttacccgc tattttaccc attagcgcgg cttaagagct tatttttgaa ttcacaatgg
     1561 tcacgatata accatcttgc gcgtccgtgg ataactttgg cttttggcag gtcgccggac
     1621 ttaatccggt catagatgaa ggttttacca aagccagtat cggccatgat gaatttcaaa
     1681 tcaaccagtg aatcaggttg tagttcgtgt tgcatgagtg ctatctccga atagggaatc
     1741 gaacctgcaa atcaggcaag aaaaaaccgc catcaggcgg cttggtgttc tttcagttct
     1801 tcaattcgaa tattggttac gtctgcatgt gctatctgcg cccatatcat ccagtggtca
     1861 tagcagtcgt tgatgttctc tgcttcgata actctgttaa atggctctcc attcctccat
     1921 tcacctgtga ctcggaagtg catttatcat ctccataaaa caaaatccgc cgtagcgagt
     1981 tcagataaaa gaaatccccg cgagtgcgag gattgttatt cagtgctgat atttaccttt
     2041 atagcgaaca cctttaccgg tttatcgccg aagtgcggat gtgtgattgt tttcacttca
     2101 tatcctccat acggaacatc aattctgcga ctggagtcgt cgcgcttcgg atatcccttt
     2161 gtgataatca ggcggtcata ctcgcgtaac ataattcgct tattccagta gtcattacac
     2221 aagcgatact cttccgtttt ctctccgcga atcatggcat cgaagtattc acctttgaca
     2281 gcaagttgca ggttagccat taccgcacct ccagtctcca taccgcctga ccaatccggc
     2341 tggcataggt atctttggat actgctccgt ctttagccag ctccataaga attttgcgca
     2401 aatctgccga acgccattct tcatcaggaa attccttctc cattgccaac cgcagattcc
     2461 aggttgctat cgtgaatgga tattccccgc cgagagcttt ctcttgcagg gcagcccggg
     2521 aacgcatcac ctgcaaaacc ttctctttta catctatcat ttcgcctcct gcggcggctc
     2581 tggtagtggc atccagtgtg acggctcaca taccccctca acaccattca tgtaaaagaa
     2641 ttgaaataac cctttacctt tgtgaaaccc taccatctgc tcttttgtgt ctgaacaata
     2701 aaccaaaaag ctaccttttt tgtaggccac atacgcagtg caatctgact caatcaggct
     2761 ttctgtaggg atgtactggc aatcaacgtg ccacactgcc attgcatcca cgctatcagc
     2821 gcaaacaggc tgatcgatat ctcgcccaca attccaggct ttttgggctt cttccagcgt
     2881 gtaaacatga gcgcgatcga tatcagaact gtaaccattg ccgttatggc aatggaatga
     2941 agcgttattg cccacagttt cacgcgagca catcatgtaa aaacggttag tcactggctg
     3001 cctcctttgc gccacatcgc attcagatat ttgttttcat tcactgatgg aaaagaattt
     3061 ctcttaagta attcctctct cgatggcatt ggctttacgc gttggcgaat aatcatttct
     3121 gccggaagaa tgtcgggatt gtatgcaagt cctctcatgg taaattcctc agtcattact
     3181 gatagcgcca tagcgtgagc ggtaattacg caggcgcggg tcaatttcag ggaagtgggt
     3241 atatgtggct ttgcggaatg gtcggattga tgtctggtaa attcgctcgc gttcttcttt
     3301 ctctgcaagc catatacagt ggcgaaattc cttttcctct ttcgtttcct gcggtagaga
     3361 cattattcga tcgtagtttt ttctgaattt atccagcacc tccgatacgg aattgccgga
     3421 acagcggcgc gggtcatccg caccatacag aggcgctggc ataattaaat ccttattttt
     3481 ataaatcaga atgggatgga atcgtcgtat acaggagtgt tctgctggtt actactttgc
     3541 tgctgcgggc tgtttcctga agttgcaaat ccaatctttg cattcagtaa ttcaagagtg
     3601 attgattgac cattttgccc ctgataaaca tcaaccctga tgttttctcc ggtaatttcc
     3661 acaatgccac cctcaaccag aacgctacgg tagtaatccg cttgcgctcc cggcttggca
     3721 aatataacgg cgctgtagtt tgtccattct ttctttttag tctggcggtc gtaatactga
     3781 acgccagcac ggatgttaaa tccgatattt tccccggcct gaaactctct tgcgggcttg
     3841 tttagtctta cagtaatcga atgtgccatt aagcagccgc tccttctaat tcatctcgtc
     3901 tgatgttgta aacgtcctgc gctttgtgct gctccggtgt gccttcgagc atcttccacg
     3961 ctttggcgaa cgcctgttta agctcttcta cggtgttttt ctgcattgct gcgtcagtga
     4021 atgcctttaa aacctgttca ggtgtaggtg atggctttga ttgctttgct gctgcgttct
     4081 gctgatgttt atgctcgtct gtatctgcat ctttcgcatc atctatgccg aacaaaccat
     4141 tgaggcaata cttgcgtgca taagagcttg tagctccagt aacttgtgca gaatccattc
     4201 ctttcttgct ttcttcctct cgtgcaaggg cggttgccgt atgactgttt tcgccatcgg
     4261 taatagttgc cgtggctttc acgtaatacc gatcaccaat caacacaact tcatcgctga
     4321 ttgataaaaa caggccattc agtagcggct taacaccctc aagaatgtct tcgcagcttc
     4381 tgtatttata tttgccgaat gagttgtatt ggttctttgg cgcgttcaga ttctcctgaa
     4441 tagctgccag tcttgcgtaa aattctttgc tcatatgatt gttctcagaa tggacacggc
     4501 ccaaggaaat aacgctgatt taatacttca gtctttgccg catttaaaaa tacgcgaaca
     4561 ccttcacgat ctcccttctg gcgatacatt aacgcctgct gcgtgtacat gcgtctctgt
     4621 aacttgctct ccttcactgt ggttgcaagt gacatgaata tctccttcgt taccgattaa
     4681 atctttcatc tgacgaatga attcttcgtc tgaccagtta tctgtaaaac tcatttcctg
     4741 cgatacaacg gaaggttgat agctgatttc atcgctttat ttgcttcaag ccacattttt
     4801 gaatcaccaa taaatctggc tattactgct ttgttctgtg cagcacgaag catctggtga
     4861 ttgatggcta tttcattgcg cataacgcct ccagttgttt ctttgctgct ctgattaatt
     4921 gtttaactcg gcgtgataat tcagattcgt gcgggtagaa agcggacatg acgccgctac
     4981 ccgcgagctg aaagtgcatc atgggtaact ccttatattt gattgcataa cgaaaacgcc
     5041 tcgagtgaag cgttattggt atgcatataa aaaagccctc acactggagg gcaaagaaga
     5101 tttccaataa tcagaacaag tcggctcctg tttagttacg agcgacattg ctccgtgtat
     5161 tcactcgttg gaatgaatac acagtgcagt gtttattctg ttgtttatgc caaaaataaa
     5221 ggccgactat gcggcctcag aatggtttat atccattttt aaaggcgaaa tcgtatgcct
     5281 catccttagt gctgaagtat tcataatcag caaatccatc acgataaaac ttgcagcacc
     5341 aatgatgcgg aatgccatcc tcaggaccat agtcttgatg gaacacatcc tcgacgatga
     5401 tattttcatc ttttcccata tatcacctaa aataagtggt ttgctgccaa aacaatgaac
     5461 catccggaaa ttccagatag ttcataattc actcttcaat acttccaact tactaatcgc
     5521 cgatagatat ccgcgctgat agggaatcat cattccttcg agcttgccac ttcttaactc
     5581 ctccctgagc aattgtattg cttgatcaat aacctctgac ttagcgtcct ttatggcttg
     5641 cttgcggggc tttgctttct gctttggcag atttctcaag catgatggaa tgtatgtctg
     5701 attcaccact tacctcgccg tcagttgttt tgatttccgg tatcctgccg cgtaaatagc
     5761 tacgtttggc aggcaaacac ttccactgca ttcatctgcc ttcttgcagc gaaggcttcc
     5821 gagtgatgct gctttgtctg ctctgacgca accagagagc tttcgcgcaa tttttcgcgc
     5881 cagtcgctgc tcttgcattg cctgttcacg ttgagcctgt ctgcgtgctc tgcggcgatt
     5941 tctggcgtta tcgtcagcca gatatgtaat gactactgtc atgttgacct ccgatgattg
     6001 actttggtga ttggatggcc ggtgctgaat tccggcttac tggttagagc gcccgcacta
     6061 ccagtgacgc tgtcttgagg cgcagattgg ttactgcttg ccatgagcgc tgtttataca
     6121 ttggtcgagc atcagcctgc tcattcatcc aatcccaaag ccaactactc tttgactcac
     6181 actctcgcag tgagcgcgct catgcccttg agtctctgcc gcttcatcgc cgctgataac
     6241 cggtgcgcgt ctggcattcg cgctgcttta ccggagctac ttttaatata ttgaccctaa
     6301 cccgatgcta agcaggctcg ctcaatggcg actcagggca gcatcattac tgctgcattt
     6361 cctttcggct gcggtctaac cgcgttagtg caccattacg gcacctcctg tttggttaaa
     6421 ctcagttccc gcatttcggc gggacaatcc catcaatgtt aaagagcctg ccaatctgtt
     6481 ccgtttggct tccagcgtcc tgctgatggc taaagaatac tgtaggtatt ttattgtgta
     6541 aatacccaag gtatttattt ttgatgaaat aatgataaac aaatgaatac aaaggatatt
     6601 tatttgtttc ggtgtctgct tgttcagtgc tttttatgcg ggatatgtga agtggatccc
     6661 gatagctatt gctgccggga ttatgggtta gtcagcgaag gttaagacga gaattacctt
     6721 aatgatgtct gctacaacag acacggccat agataaacca aagacgatcc aagccataga
     6781 gatgtcttca ctaccatcgt atagggttcc gtaatcactg gtgtaaggcg taaatgtcgc
     6841 gccttgatac aataggtata agcttgatcc atagaggata aatgcagata tcccttgtat
     6901 tgctatgatc actagaatca tgaaacgagc tgatctatgc gcccaagcct ggcttatttt
     6961 ttctgataga gatttcgcaa taaaagcatg cgctaagccg taaattgttg agattgccaa
     7021 catccccaaa aagcttgcta tagcggttcc aaccataagc gcccccttgc gtgatcaaac
     7081 cagtctgagt tttgtctcaa ttgcaacgcc tataatcttg cagtttccat tgattggcac
     7141 gagaggccat gcaggattaa gtcccttgag gtatttattt ccgccgtcga ttatcagctt
     7201 cttgaatgtt gcttcgttag agtcagaaag ttttgctatg accaagctgc cgttgatcgc
     7261 ctcccttccg gtatcgaaaa gaacgaatgt tccctctgga atgcttaacc caaccggtgc
     7321 cgtcattgaa tcaccttcca ctttaagcca gaacgcatta ccttgaatat gcgcgtcaga
     7381 ctcaagccaa acatctatgt ctttaatggt gtatggttcg catgcttcac accacgagcc
     7441 agcctggata ctgcttaaca ccggatacct ctttcctgct ctgtattccc ctgcatacct
     7501 tacgttggca tcgctcttaa ggctttctgc ctgttctgca accttggcag caattgactg
     7561 gctaaaatca gcaattgaga cttgcaacag tcgtgcaaaa ccagatgcaa cctcaacgtt
     7621 tagcgcgttt ctgccattaa gataatgccc taccgctcct tgggtgatac caagttcatc
     7681 agcgattgag tattgggtta ttcccaactc tttctttttt gactcataca aagccttaag
     7741 ccgcttagcg tcttcgagct gttctgtcgt cagtgatttt ttattttcca tagcttaatt
     7801 ctaatagcta aggtacttaa actaaaaata ccctgagtat tgattgcttt gaatacctgt
     7861 agtattcttt gttcatggtt aataacggag tgtgcatatg attcgaatga cacttgccga
     7921 ttacgccaaa atccatggac aggctaaagc agccagtgac tttggtgtaa tccagtgcgc
     7981 tatcagcaag gccattctgg caggccgtaa cattatggtt acggtaaagc ctgatggcag
     8041 tgtgattgga gaggaagttc gtcctttccc aagcaacaag aaaaacaaat agtaacaccg
     8101 ctctttaaca gtcatggtcc tcattcccgc cgaaatgcgg gaatacaacg cgcatcagtt
     8161 gttgcgcata acttcttatt tgttaaggaa atacttacat atgcaactta caagtactcg
     8221 caagaaagcg aatgcaatta caagcaacat cctaaatcga attgctgtac gtggtcagcg
     8281 aaaggttgct gatgcattag ggatcaatga atcgcaaatt tcgcgatgga aagacagctt
     8341 tatcccaaag atggccatgc ttctggctgt gctggagtgg ggtgttgaag acgaggaatt
     8401 agcggagctg gcaaagaaag tagccatggt gctgacaaaa gaaaagcctc aagactgcgg
     8461 caacagtttt gaggcctgat gtagaaagac tggatcaatc cacaggagta attatgacaa
     8521 aacgtcgtaa gaaataccag gaaaaagaag agattcgaca ccctgattca cctgagggat
     8581 tagtggtagc cgcagcaaat aacagggcgt tcgcagagcg ccttgttggt gtttacagac
     8641 tagccaaagc aggagtgaaa catgggcgtc gttaagttag ctgattacag gcataaccct
     8701 gtacaacatc aggaggcatc cagtatgggg tatgtctcta tacaccgcca gtttatggac
     8761 agcaggctct ataaggactc tcaggcagta catctttggc ttcacttaat cctcaaggct
     8821 aatcacgaat ctactgtcgt caatacggat atcggtccga taactgttga tcgcggtcag
     8881 atgataactg gacgcccgtc gctggtcaga gaaacattca tccccgacaa caaagttcgg
     8941 agcttattgc ggacttttga gtcgaaagga atgcttaata tttgctcgat ggggaagaaa
     9001 tttagcctgt ttacaatcgt taaatatgac gattttcagg caaaaaattg tccaacggtt
     9061 gtccaacggt tgtccaacgc aaacagcagt aatggcgcgg ctctcagcgg agattatcca
     9121 acggttgtcc aacggttgtc cataaacaat aatataaata atatctctaa tactgacgta
     9181 ttagagagtg ccacagcaga caaaaagtct gacaagaaaa aaccttccgt tagctgtcag
     9241 gatgttgtcg atgcttacca cgaaatcctt cctgaagcgc caagaatccg cgcactgaat
     9301 gacaagcgta aaaaccagat ccgaacgttc tggcgcaaag ccggagtgat aacccgccag
     9361 cttgacgggc atgggttcac gatgcaggac tggagaaatt atttgagcta cgtaggcgaa
     9421 aattgccgat ggatgttcga agagcgccca aaccatcagc gcggaaccgt ctggcacaaa
     9481 aagggatttg atttcctgct taacgataat acctacctga aagttcgtga gggtgaacac
     9541 gatgaccgat aatttttatg cgccgcccca tagcatcgag gcagagcagg cggtgattgg
     9601 tggattgctt ctggatgatg acagcagtga gcgcgtccag aaagttctgg cgatgctgaa
     9661 gcctgattca ttttacagcc gaccacacaa aatcattttc gaagaaataa ccagaatgca
     9721 ccgggagcaa aagccagtag atggcctgac gcttttcgat gaactggagc gtaaatcgtt
     9781 aacggcgtct gttggcggtt ttgcttatat cgctgagatc gcaaagaaca cgccaagcgc
     9841 cgcaaacatc gttgcctatg caatgcaggt tcgcgaaacc gcaatggaac gctacgccat
     9901 caaccgcatg actgaagcga cggaattgct atattcccgc aacggaatga ctgcaacgca
     9961 gaagtacgaa gctattcagg cgattttcac gcaactgaca gaccatgcaa aaaccggatc
    10021 gcgtcgcggc cttcgctcat ttggtgaggt catggaagac tgggttagcg accttgagaa
    10081 gcgatttgac ccgtcaggcg aacaacgagg aatgagcaca gggatcccat cgctggacag
    10141 gatgctgtca ccgaaaggtc tggtgaaagg ctctctgttt gtcattggcg ctcgccctaa
    10201 gatggggaaa acgacgctat acagccagat ggcaatcaac tgcgcagtgc atgagaaaaa
    10261 gcccgctctg atgttcagcc ttgaaatgcc aggtgaccag atactggaaa aactggtggg
    10321 acagaagtca ggtgttaacc cgaatatttt ttaccttccg gcgacaaatg acgctgatga
    10381 cggctatcag ggtgattacg atggtgactt caacagggcg atcgaaacag ctaatcgttt
    10441 gagtgaaatc gacctgctat acatcgacga cacgccggga ttatctctgg ctcaaatcgt
    10501 tagcgaaagc cgtcgaatca agcgagaaaa aggatgtgtt ggcatgattc tggtcgatta
    10561 cctgacacta atgactgctg agaaggccga tcgcaacgac cttgcttacg gcatgatcac
    10621 caaaggactg aagaaccttg ccaaagagct tgattgcgtt gttgtgcttc tgacacagct
    10681 taaccgcgca ctggaaagcc gaaccaataa acgcccatta ccaagtgact cgcgagatac
    10741 agggcagatt gaacaggatt gcgattattg ggtggggatc catcgtgaag gcgcttttga
    10801 tgacagtgtt ccacctggtg aaactgaact aatccttcgt ctcaatcgtc atggcaatac
    10861 cggcacggtg tattgcattc aggcaaatgg cgctatttat gacacagacc aacagtctgc
    10921 tgaaatgcgc cgccgtgaac gcgaggaacc gcagtccaag aagaaaggag gattctgatg
    10981 accatctaca tcactgagct aataacaggg gctatttaca cagtagccct tttttattgg
    11041 attaagaacg agggggatcc tgatggacac cgttaacgga atgtgttcag acgcaccgcg
    11101 tgccaaaaaa tgtaaatgcg gaaaatcacc gacaatattc gacatggaga acgggtgcca
    11161 aatctactgc gctaaccacg ctgctgtggc ggccgcgaat tatcgcagtg cggtaacgga
    11221 gtggaataac ctgaaatctg ttagagaggg aagtcatgaa aaaactaacc tttgaaattc
    11281 gatctccagc acatcagcaa aacgctattc acgcagtaca gcaaatcctt ccagacccaa
    11341 ccaaaccaat caatcaaata actcagcaga gacttaaaga agtccttctc tacgatgagt
    11401 taacagggaa attcacatgg aaagtaaaga aatgccagag aatgaatgct ggggatatcg
    11461 cagggcataa gagtagcgaa ggctactggg taataaaagt cgatggaaaa ctttataagg
    11521 cacacaggct ggcgtggctc tatatgaatg gcagtctacc aaaaagtgat atcgaccaca
    11581 taaatctcgt tagggatgac aatcgtatag caaatcttag attggcaact cgatctcaaa
    11641 acatccaaaa cgtaaacaaa aaggcaaata acaaatcagg atataaaggc gtttcatggg
    11701 ataagaaatc aagaaaatgg agggcgcaaa tcgtaatcaa caagaggaaa gttaatcttg
    11761 ggttttacga tgacccaaag gaagctcata aagtatatgc aaataaagcg gatgaatgtt
    11821 tcggcgaatt tgcgaggtat taaaatgaaa tgtgtcaaat atcaccttac taatgaatct
    11881 ataagatata acgccattca gtatcttagg acatgcgatt tagacatcat tgtcgagttt
    11941 aagcagcgca accgcagctt agaccaaaac aggaagcttt gggcttgcct tggtgacgtc
    12001 tctcgtcagg ttgaatggca tgggcgctgg ctggatgcag aaagctggaa gtgtgtgttt
    12061 accgcagcat taaagcagca ggacgttgtt cctaaccttg ccgggaatgg ctttgtggta
    12121 ataggccagt caaccagcag gatgcgtgta ggcgaatttg cggagctatt agagcttata
    12181 caggcattcg gtacagagcg tggcgttaag tggtcagacg aagcgcgact ggctctcgaa
    12241 tggaaagcgc gatggggaga tcgggctgca tgactatcaa atcaaatacg ccggcacacg
    12301 acaaggactg ctggcaaacg ccgctttggc tttttgatgc actggatatt gagtttggat
    12361 tctggctgga ttcggctgcg agcgacaaaa atgctctgtg cgctcactgg ctaactgagg
    12421 ccgacgacgc gctcaattct gagtgggtaa gtcacggtgc aatctggaat aacccaccgt
    12481 acagcaatat caggccgtgg gtggaaaaag ccgctgagca gtgcatacaa cagcgacaga
    12541 cggtagttat gcttgtgcca gaggatatgt cagtcggatg gttcagcaag gctctggaga
    12601 gcgttgacga agttcgcatt atcactgatg gacggattaa ttttatcgaa ccatcgacag
    12661 ggctggagaa gaagggaaac agcaaaggct ccatgctgct gatttggcga ccgttcatca
    12721 gtcctcgacg gatgtttact accgtatcca aagcggcatt gatggcgatc gggcagggcg
    12781 tcaggagggc agcatgaggc gacagcgacg aagtattacc gacatcatct gtgaaaactg
    12841 caaatacctt ccaacgaagc gctccagaaa taaacgcaag ccaatcccaa aagaatctga
    12901 cgtaaaaacc ttcaactaca cggctcacct gtgggatatc cggtggctta gagaacgtgc
    12961 gaggaaaaca aggtgattga cccaaatcga agttacgaac aagaaagcgt cgagcgggct
    13021 ttaacgtgcg ctaactgcag tcagaagctg catgtgctgg aagttcatgt gtgtgagcac
    13081 tgctgcgcag aactgatgag cgatccgaat agctcaatgt acgaggaaga agacgatggc
    13141 taaaccagcg cgaagacgat gtaaaaacga tgaatgtcgg gaatggtttc accctgcatt
    13201 cgctaatcag tggtggtgct ctccagagtg tggaaccaag atagcactcg aacgacgaag
    13261 taaagaacgc gaaaaagcgg aaaaagcagc agagaagaaa cgacgacgag aggagcagaa
    13321 acagaaagat aaacttaaga ttcgaaaact cgccttaaag ccacgcagtt actggattaa
    13381 acaagcccaa caagccgtaa acgtcttcat cagagaaaga gaccgcgact taccatgtat
    13441 ctcgtgcgga acgctcacgt ctgctcagtg ggatgccgga cattaccgga caactgctgc
    13501 ggcacctcaa ctccgatttg atgaacgcaa tattcacaag caatgcgtgg tgtgtaacca
    13561 gcataaaagc ggaaatctcg ttccgtatcg cgtcgaactg attaaccgaa tcgggcagga
    13621 agcagtagac gaaatcgaat caaaccataa ccgccatcgc tggactgtcg aagagtgcaa
    13681 ggcgatcaag gcagagtacc aacagaaact caaagacctg cgaaatagca gaagtgaggc
    13741 cgcatgacgt tctcagtaaa aaccattcca gacatgctcg ttgaagcata cggaaaccag
    13801 acagaagtgg cacgcagact gaaatgtagt cgcggtacgg tcagaaaata cgttgatgat
    13861 aaagacggga aaatgcacgc catcgtcaac gacgttctca tggttcatcg cggatggagt
    13921 gaaagagatg cgctattacg aaagaattga tggcagcaaa taccgaaata tttgggtagt
    13981 tggcgacctg cacggatgct acacgaacct gatgaacaaa ctggatgcga ttggattcga
    14041 cacccaaaaa gacctgctta tctcggttgg cgatttggtc gatcgcggtg cagagaacgt
    14101 cgaatgcctg gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca
    14161 aatgatgatt gatggcttat cagagcgtgg aaacgtcaat cactggctac ttaatggcgg
    14221 tggctggttc tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa
    14281 agcagatgaa cttccgttaa tcatcgaact ggtgagcaaa gataaaaaat atgtcatctg
    14341 ccacgccgat tatccttgtg acgaatacga gtttggaaag cctgttgatc atcagcagtt
    14401 aatttggaac cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaataaaagg
    14461 cgcggacacg ttcatctttg gtcatacgcc agcagtgaaa ccactcaaat ttgccaacca
    14521 gatgtatatc gataccggcg cagtgttctg cggaaacctc acattgattc aggtacaggg
    14581 agaaggcgcg tgggcataag agaactaaac ctcaccaaag aacagcatga gtggctgaat
    14641 ggctggcttg aactgtgggg cgcatgggtt tattcaggtc gtctggaaaa gcgcatgagc
    14701 agcgtaatag cgaagttcat ggagagcgta gagccgggaa gagttatgac aaggccaatg
    14761 tgtaatgatg atgatggaat gttgatttct caggtcgtcg attccgtcat gtacattgac
    14821 aagaaagcct ttggcatcct cctcagctac tacgctcatg gttcatctaa gcgagcaatt
    14881 gcatcctact atcacgcgac tgcaaagcca cgcaagatgt gtggacgtgg tggcgaggga
    14941 tggagaaaac cttcaatggc aacctgtaga aacgaaattg acgacatcct gaaagcgtcg
    15001 ttatttgttt tgtaccagcc aatgcaaaat gctttcaaaa tgcgtaaacg tgttgagaaa
    15061 gttaagcatg ttgctgttaa aagtcttgac atgcaattag ccatttagcc ataattagca
    15121 ggtaagctgc cgttagtgac tcttaagttg caacggtggc tttttttatt tgggtcagtc
    15181 gtataaaggt cattacggaa ggctgttaac cttcttatcg tggttcgagt ccacgctgtc
    15241 ccgccaaata tgctggttta gctccaatgg tagagcagtc gccttgtaag cgaatgggta
    15301 acggttcaag tccgttaacc agcaccataa ctgagccgta gccactggct atcctgaatt
    15361 catcagtgat agttacgctg cggcattcta cgcatgacct tcgtgaaagc gggtggcatg
    15421 aggttgccct aacaacctcc tgccgttttg cccgtgcata tcggtcacga acaaatctga
    15481 ttactaaaca cagtagcctg gatttgttct atcagtaatc gaccttattc ctaattaaat
    15541 agagcaaatc cccttattgg gggtaagaca tgaagatgcc agaaaaacat gacctgttag
    15601 ccgccattct cgcggcaaag gaacaaggca tcggggcaat ccttgcgttt gcaatggcgt
    15661 accttcgcgg cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt
    15721 gcgccattat cgcctggttc attcgtgacc ttctcgactt cgccggacta agtagcaatc
    15781 tcgcttatat aacgagcgtg ttcatcggct acatcggtac tgactcgatt ggttcgctta
    15841 tcaaacgctt cgctgctaaa aaagccggag tagaagatgg tggaaatcaa taatcaacgt
    15901 aaggcgttcc tcgatatgct ggcgtggtca gagggaactg ataacggacg tcaaaaaacc
    15961 agaaatcatg gttatgacgt cattgttggc ggagagctat tcactgatta ctccgatcac
    16021 cctcgcaaac ttgtcacgct aaacccaaaa ctcaaatcaa cagcagccgg tcgctatcag
    16081 cttctttccc gttggtggga tgcttaccgt aaacagcttg gactgaaaga tttctctccg
    16141 aaaagtcagg acgctgtggc actgcaacag attaaagaac gtggcgcttt accgatgatt
    16201 gatcgcggtg atatccgtca ggctatcgat cgttgcagca atatctgggc gtcgttacct
    16261 ggtgcaggtt acggtcagta tgaacataaa atcggtgacc tgattgcccg atttaaaaaa
    16321 gctggtgggg tagtaaatga agctgagata taggctggtt attgttgcct tcgttgttac
    16381 cgtcattggt tccttcatct ggtctgctgg gcattactac agcaaatatc agcacgaaaa
    16441 ggagcgtgct gatgaggctg tacgaaatgc tgaatcagca actgccatta cccgtaacgt
    16501 tctgcaatca ctgcaaatca tcaatacagt tatagaggct aaccagcatg caaaagagca
    16561 gatcgcactg gacgcatcgg gagcctcggc tgatatccgg gttgctgttg cgaatgatga
    16621 ttgcactaat cgccctgtcc ctgctggcgc agttaagcgg ctgcacgaat acgcgaacgg
    16681 tctacgtgcc ggttccggta gttccattac cagccagcct gacggctgaa acaccccagc
    16741 cagaattacc cgacccgttt acgtggggag caagtcttga tctgaatgtc gcgttgctgt
    16801 cagcgctggc gcagtgcaac agggataagg ctgatatcag gactttcgag aacaacaggg
    16861 caggacaaac cgatggcacg attaaacgtt gaagttaccc caccagacag cgaggcgctg
    16921 aacgggattt ttgcagagat tgagcgcaaa tatgcgcgtc agccgctgac accaaaagta
    16981 attgatgaaa tgcaacgcga agcgacgcgc cttgtacggc gaatgataac cacaaaggtt
    17041 acgttcgtcc gggactgaca ttacagaagc ccttcacact gaggggcttc gataatggag
    17101 cactggaatt attcatgaac agaccacacc caccatcgca ttttacgatg ccacctgacc
    17161 cgaagccgta catcagcata atgcccgcca gtgacgttgg cgagtggctg aatcagcaca
    17221 tcctgagcga tgagggtgac ctctacaacc ctgatcacca gcatttgctt gaagcggatc
    17281 tgtgctttct ctgggcgtcg aacgctttcg agaagaaagg gcgttccgtg cttgggcagg
    17341 cggaagaagt ggcaatgcgt gccggaggct ggcaaaaagc gcggatggag cagcagatgt
    17401 atgaatggtt cggcagggtg ccgcagttca tcatcacgct ggcagccgat tactgttcgc
    17461 aatgttccga tctggaattc tgcgcgctga tagagcacga gctttatcac atctgccagg
    17521 cgacagatga atttggcgcg ccgaagttca cgcaggaagg gcagccaaag ctgaagctgc
    17581 gcggccatga cgtggaagag tttgtgggcg tggttcgccg ttacggtgcg agccgggacg
    17641 tgcaggaaat gattgatgtg gcgaatcagc cagcggaggt tgctcatctc gatattgcca
    17701 gagcgtgcgg gacgtgcatg ctgcgactgg cttaaatact ggactgtata agacgaatgg
    17761 tgatttatgg ctgcattaaa atctgatgtg aaagccttca tcattcagtc gcttgcgtgc
    17821 tatgacacgc catcgcaggt ggtcgaggct gtccaaaaag aattcgggat caagatcacc
    17881 cgccagcagg ctgaatctca cgaccccacg aaggccagcg gtaagacgct cgccaaaaag
    17941 tggatcgaga tgttccacgc gacgcgcgaa cggttcctga ccgaaaccag cgacattccg
    18001 atcgcgaaca aatcatatcg cctccgcgtg cttgaccgca tggcaaccaa aaccgagggg
    18061 atgaaaaact tctccctgac ggcgcagctg attgaacagg ccgcgaaaga ggttggcgac
    18121 gcttacacca ataagctgaa ggttgaaagc actggcaagg atggcggccc aatcaagacc
    18181 gagacgacca acctcaccgc agatcaggcc gcagagattt accgcaagat gatggggtga
    18241 tcatgcctct cccgtttgaa ttcgatttca gaaaccctga ttaccagatg gtttttgaat
    18301 ggcggatgga gcgcttacag cgcattcgcc agaaccctga aatgctgcca gcgctaaagc
    18361 agttttaccg caccaacccg gcacagttca tcatcgactg gggtatgacg actgacccgc
    18421 gtaacatcga ttatggcctg ccggtcacca tcccttttct gctgttcccg aaacaggaag
    18481 agtggattca ctggatcatg gagcggcgcg aacgactgga gaacggcatc accgaaaaga
    18541 gccgcgaaat ggggctcagt tggaccgcga tcgggctggc ctgctcgctt tgtctcttca
    18601 acaaagaaat ggttatcggt ttcggctccc gtaaagagga atacgtcgac agcaccggtg
    18661 acccgaaggc gctgttctgg aaggcgcgaa agttcgtgga aacactgccc atcgagtttc
    18721 gtggttcgtg ggacgagaag aagcatgcgc cgtatatgcg cgttgagttt ccagatactg
    18781 gcgcggttat caaaggcgag gctggcgaca atatcggacg tggtgaccgt accacgcttt
    18841 atctggttga tgaggctgca ttccttcagc gtcctctgct gattgatgcg gcgttgtcac
    18901 aaacgacgcg ttgccgtatt gacctgagtt cagttaacgg catggcgaac ccgttcgctc
    18961 agaagcgtca tggcgggaag ataccggtat tcacattcca ctggcgggat gatcctcgca
    19021 aggatgaaga gtggtatcgc agggaatgcg agaaaatcga taatccggtg gtggtggcac
    19081 aggaacttga tctgaactac agcgcatcag cggaaggcgt cctgattcca tccgaatggg
    19141 tacaggctgc cgttgatgcg catatcaaac tgggtatcca gccaacaggc aaacgacttg
    19201 gcgcgatgga tgtcgccgac gaaggcaggg acaaaaatgc cttttccacc cgtcatggct
    19261 tcctcctgga gaatgtgcgg gaatggtccg gtgtgggcag cgacatttat cagtccgtcg
    19321 agaaggtctt cggtttttgc gaacaggaca acctcgaaga gtttcgcttt gatgaggacg
    19381 ggctgggcgc tggcgttcgc ggcgatgcac gcgctatcaa cgaacagcgt aacgctgcgc
    19441 gtctaccgtc aatactcgct acaccgtttc gcggtagcgg cgcggtgttt gatccggacg
    19501 acgaagcggt gcgcggtgac aacgggcaag ccgcacgtct gaacaaggac ttcttcgcta
    19561 acgccaaagc ccagagctgg tggcggttac gtaaactttt tcagaatacc tggcgcgccg
    19621 tggttgaagg tatggcttac aacccggacg aaatcatctc aatcagcagt agcatggcac
    19681 tcaaagataa actcatcatc gagctttcgc agccgaccta ttccattaat ggtgtgggaa
    19741 aaatcgttat tgataaacag cctgatggaa cccgatcgcc aaaccttgcc gactcggtga
    19801 tgatcaacta tgccccaatg aattcagccc tgaacatctg ggagctgcta gggagacagg
    19861 cctgatggca cgaaacaaac aagccctgcg gcgaactgcg caggccaccg ctgatggcta
    19921 tgagaacttt attgcccgcg tagggatgca gacgcctaac cagcactcag catcgaccta
    19981 ccgggctaac ttcaccagcc gcaaccgcat gctggtggaa tggtcctatc gttcgtcctg
    20041 gatcatcggc gaagcggtcg acgctatccc ggacgatatg acccggaaag gcattcgcat
    20101 cacttcggag attgacgcca aagaccgtgg caccctggaa gcgcagctgg atgagttgca
    20161 gatctgggat gcgctgaacg acgtgctgaa atggtcgcgc ctctacggcg gcgcggtcgg
    20221 cttcatcatg atcgaggggc aggcaccaat gaccccgctg cggctcgaaa ccattggaga
    20281 aggcaagttt aagggcattc tcccgctcga ccgctggatg attaacccgg tgctgacccg
    20341 ccgcattaaa gatatggggc cggacctggg taaacctgag ttttacgacg tggtgaccac
    20401 tgcaacgggc atcccggcct ggcgcatcca tcacagccgc ctgattcgct tcgatggcgt
    20461 cacgctgcca ttccagcaga agatgaccga aaacgaatgg ggaatgtcgg ttgtagagcg
    20521 tatctgggat cggcttactg cgttcgacag cgctactgtc ggcgcggcgc agctggtcta
    20581 caaggcgcat ttgcgtacct acagcgtgga gaagctacgc gagcttatcg cacttggtgg
    20641 tcctgcgtat gaagcgttgc tgaagaatat cgacctgatt cgacagttcc agagcaatga
    20701 aggcatgacg ctcatggact cgcgggataa gtttgaaacg catcagtaca gcttcagtgg
    20761 tctggatgac atcctttcgc agtttgcaga acagattagt ggcgctgttg gtattccgct
    20821 ggtgcgcctt ttcgggcagt ccccgaaggg attttctacc ggcgatgcag accttgccaa
    20881 ctattacgac cgggtgagct cgttgcagga acgccgctta cggatgccga tgcgtcggat
    20941 actggacatt atgcaccgct cggaactcgg taagccgctg ccggacgatt tcacgtttga
    21001 gtttaacccg ctatggcaaa tgtctgacgt tgaccgctca acggtggccg taaacaccac
    21061 caacgcgatc agcaccgcgc tgggcgacgg attgatgacg cgtaaggcgg caatgaccga
    21121 cctgcgcgaa aactctgacg tcaccggcat cggggcatcc attaccgacg aggacataga
    21181 gaatgccgaa gacgaagcgc cgccaggcat cggcgaactt ggcgacaaac cgccagagtc
    21241 gccaggcgga aatccgatat cgaacgagcc tacggcagat agcgcgggcg gtcggggata
    21301 tcgtaaatgg tcgctacgat ggttcaaatg acagtgtcac cgaaataatg gatgcgctgg
    21361 agcgctacag cgaaatcatc accccctggg cgacgaaggt tgctgagaac tttaccgccg
    21421 acattgcacg ccagaatgaa aagcagtggc gtcagcacag caggaacatc agcgcagagc
    21481 tgcgcaacat ggtcgaccgc gccccggtag gccaggtgat gaaatccatc gtcgccgagc
    21541 aaattaagta catcaagtca ctgcctcttg aggccgccga tcgggtgtat gacattcaga
    21601 acaaggccat cgaggccgtt gtgtctggtg gccgcgctga accattcgcg aaagagatag
    21661 cagcgtccgg tgacgtgtca cgctcacgag cgaaccttat cgcccgtacc gagcttggac
    21721 gtgcaaccgg cgcgctggat caggcgcgtg cgctgtcaat cggctcgaat ggttatatct
    21781 ggcgtacagc cgaagatggc gacgtccggc attctcatcg ggagatggaa ggtaagtttg
    21841 tcgaatgggg caaacctcca acgcttgatg gcatgaccgg tcacgctggc gagctcccga
    21901 attgtcgctg ttataaagaa atcgtttttc ccaaccctca ttcttatctc gcctgaatcg
    21961 caggtaaacc atgaaatatt ttttcaatac ccggctgggg gaaacccgct atcagctggc
    22021 tgacggctcg ctgctgtgca aagacgtgcc gataggtcga acgggtaagc agctctacgg
    22081 cgctgccgat ctgccaaacc tcaaacccga caagctcggt gagatagtcg taacgcgttc
    22141 tcctgagcag gtattccatc cggccacgct cgcctcattc gaagggatga gcatcacgat
    22201 cctgcatcct gaagatgaaa acgggaatgt gcggctggta aatcccgaga actggaaaga
    22261 gcttgcggtc gggcacctcc agaatgtccg gcgcgggacg ggtgagcagt ctgatttgat
    22321 gctggctgac cttatcgtca aagacgaaaa cgccattcag cttatcgaag atggcctgcg
    22381 tgaagtgtcg tgcggctatg acgcggagta cgagcagacc gagccaggta aagccgagca
    22441 ggtcgatatt accggaaacc atgtggctct tgtccccaaa ggcagagccg gaaatcgttg
    22501 tgcaattgga gacagagaca caatggcaaa tcaaaagaaa aactggtgga accgcatgcg
    22561 tgcagccatc aagacaggag atgccgacac catgaacgaa ctggtggagt cggctcccgc
    22621 atcggttaca ggagatgagg gggatttgcc gcagggcgtt aatctcaaca tcaacctgtc
    22681 cccgcagcaa ccactaccgg acaaagcacc agagatgggt ggaggtccaa ccggcgacag
    22741 tgatgatgac ctcaaaacat tactgaaagc cctgctggct aagctggaag gaaatgcgac
    22801 gggcgataac gacaataagc ctgacgataa tccgaccggt gacggcgagg acgatgaaga
    22861 ggaaaccacg attactggtg actcagcctg gcgtgccgaa gttatcgttc cgggtatcga
    22921 tctgagccgt aagatgaaac cgaccgcgtt caaacgcgag gttctggctt ccgcagataa
    22981 aacgctggtt cgccagatcg tcggtgatgc ggatatccgc aaattgccga aacaatcggt
    23041 cgacatggcg tttaatgccg tgtctgagat tgccaaaggg cgaaacaccc gcgccaccac
    23101 cggcgatgca cagcgccaaa atatgggcat gaccagcatc gcttccctga acaaacaaaa
    23161 cgccgaattc tggtctaacc gcaaaggata atccaatgac tgcatatctg taccggatgc
    23221 ctgttggcat tgccggggct atctctcgcc cgcaggactt aaccgtcgaa ccggtggtcc
    23281 ttaaatccga taacgccttc gctgcctatg ggctggctgg taaatacgat gatgacggtt
    23341 ttttcgtgcc gctggcagat ggtgataccg cagacaaggt gaaggggatc tatgtgcgcc
    23401 cttatccgac cacttcgcag ccggacatgg ttcgccaggt ggggagtggc aagaacttcc
    23461 cgggcgacgc aatgaagcgt ggctacgtga ccgttaatct cggttctgat tttgatgcca
    23521 gcaccatcaa aaaaggcgac ccggtatacg ttgtcgtctc cactgatgaa tccatcaaag
    23581 tgccgctggg tggattcatg gccacgtcag tcagtggcaa aaacgtggtg ctgaccaacg
    23641 ctgaattcac aggtgccggt gatgctaacg gcaatgcaga aatttcctgg aagatttaag
    23701 gaacagacga atgattactt ttgatcaggc aaccgttgac agctctggtg cctttctcat
    23761 cggggagctg gaacgactcg accagacgct gaacctgcca ctggtggggt acacctggac
    23821 ccgcgatatc caactgcgtg aagatgtctc catcgcagat gacatttcca gctggacgaa
    23881 taccagcttc gccgctgcgg gtactggcgc aaatccgaat ggcaaaaact gggtaggcaa
    23941 agactcaacc gctattgctg gcgtgaacgt ggatatcggc aaatccggta acccgctgaa
    24001 cctgtggggg atggaacttg gctggacggt catagaattg caggctgctc agcaggtcgg
    24061 acgcccgatt gatacgcaga agtatgacgg gatgcaactg aaatggcaga tggataacga
    24121 tgaacaggtg tatgttggcg attccgcatt aaacctgaaa ggccttgtta ccctgaacgg
    24181 tgttcctgtc aacaacgctg ccaaaacgtg ggcaacctca acaccggacg aaatccgcgc
    24241 aagcattaac caggtgctgt ctgatgcgtg ggccgcttct ggttactctg tggttccgcg
    24301 tgatttgctg atcccacctg agcagtttgc tctgttgtcc agcatcatcg tttcatctgc
    24361 gggtaaccag tccctgttga cgtaccttca gaccaacacc atcagctatc accagaacgg
    24421 tgttccgctg aatatccgcg cggttaaatg gctgaaaggc cgtggtgtgg ggaataagga
    24481 tcgcatggtt gcgtacacca acgataaaaa atacgtccgc tacccgctgg ttccgcttca
    24541 gagcgtgccg gtgcagtatc gcggtctgta tcagatcgtc acttactacg gcaagctggg
    24601 tgcagtcgag ccagtgtaca aagaaaccat ttcgtacgtt gatggcattt aacagccaca
    24661 tggcccctgg cggggccatt aaggatgacc tgatggcaaa aaataatgca gtaatacacg
    24721 tacatacccc gtttgtgctc acgcttcccg acggttcacg gcgcgagttt cttaaaggcc
    24781 gtcatgctgt ggaggaagac gttgccacgc actggttcac tcgtgcgcac gcggaggtat
    24841 ccgttggcaa agccacagac gcgcgtaacg aggtaaaaaa tgccaaagaa tcaaagtctg
    24901 ccagcggtaa gtgattttcg ccgcgacttc ccgcagtttg ctgaccctgc caaatatccc
    24961 gaagcgcaaa tccagtttcg tctgaatctg gccgatgaac tgctgagcga aaacgtcacc
    25021 ggcaaaaagt tgtttccgta ctttgccgga ttgttcgttg cacactacat gacactctgg
    25081 gcggcagaca gccgggcgat gctggctggc ggtccgggcg gttcaaccaa tggtgttcag
    25141 tcctcaaagt ccgtggataa ggtaagcgtc agctatgaca ccagcgcgac gctaaaccat
    25201 gacgcaggct tctggaataa cacccgatat ggcgctgaat tttatcagct gatcacgatg
    25261 ttcggtgcgg gcggtcgcca gctatgagct tcaaaagcgg tgtaacaacg agggtggata
    25321 acgctcaggc cattctggat gcgctcaaat ccatcagtaa aaaagaagtg ctggtgggta
    25381 tcccggaagc agacagcgag cgtgaggatg ttccgtttgg taatgccggg atcggttacc
    25441 tcaacgaata cggctcgcca gcgcaaaaca tccccccacg cccgcacctg atccccggcg
    25501 ttaaatcggt agaggaacag acgttgccgc agctcaaaac agcggcgcag gctgcgcttg
    25561 atggaaatgc ggcgggggcg gaaagagcgc tcaaccgcgc cggaacgctg gccgctaatg
    25621 gcgtcaggcg ttacatgact attaccggct ttacaccgct tgctgatagc accgttgaag
    25681 cccgcgcgcg tcgagggcgt aaaggggcga aagcggagct tgcccgacgc gctgctggcg
    25741 agtcccccgg aaccgatctg gtgaaaccgc taatcgacac cgggcaatat cgcagagcga
    25801 ttacccatgt tgtgagggat aaagatgccg actcttgatg taacagacgt gctttttgac
    25861 cccgattttt gcgacttcaa cctgtgggta acgcgtcgcg tgcaaacggt ggacgatgac
    25921 gggatcggca gtgacagcga agttaaaacg cagtttgccg gagttgttac cgttgaccgc
    25981 tccctggaga accgccgtat gcagtccggg caagttatca gtggcgcgat tcttatcgtg
    26041 acaactgagc ggctgacaca ggggcagact ggccgtgatg ccgatatcgt gacgtatcag
    26101 ggccgtgatt atcgtgtgac tttcgtcgac ccgtatacag cttatgggtc cggattcgtt
    26161 caggcgcatt gtgagttgct gccgtttgat gggggaattc cggttgagca ataacaccag
    26221 cacagagcgc ggatggctga taccaaccag tggcgatccg gattatgacg aagcgctcga
    26281 caggctgtta agccagtgga tgcgtaacgt ttccggtctg tctgccggga tggttcgccc
    26341 gcgctggcag aaagagcagc cgccactgct accggttgaa acgaactggt gtgcgtttgg
    26401 ggttatcgga tggtcaggtg atgacagtcc ggcattcacc agacagaccg atgatggctc
    26461 tcagctctgt cggcatgaaa cgattgagtg tatggcttcg ttttatggtc cggcggggat
    26521 ggtgtatgcg tcccggtttc gtgacggtat atctgtaccg cagaacaacg cagcactgaa
    26581 tgcgctgggg ctgtctcttg gcgattacac aggtctgact cccttccctg aacttattaa
    26641 tcagcaatgg gtccgccgct acgatatgaa ggtgcgtctg cgccggaagg ttgtgcgcga
    26701 gtacggtatt aaatcgctgg tggaagcacc agtcatcttt ttcggagatt aagctatggc
    26761 acagggcttg cctgtatcaa acgttgttaa tgttgatgtg atcatgtcgc cgcgtgcagc
    26821 atcagggcga aattttggtg cattactcat tctcggcccg tccacaatca ttccggtaag
    26881 tgagcgcatt cgccgttatt ctgccgcgga agatattgga aaagattttg gcgtggaatc
    26941 accagaatat aaagctgcgc aggtgttttt ctcacaatca ccgaaacctc aggaggtttt
    27001 tgttggtcgt tgggtgaaaa cgaagggaga cagcgaacag gccacgcctg agacgctgga
    27061 gcaggctgtg aatgccatgc ttgattatac ttcatggtat gggctgggga ttgcagacga
    27121 tgcagatatt ccggatgcag actggctgaa agtggctgcg gcgatcgaat cctcttctgt
    27181 aagccgtatt ctggcgatta cgacaagcga tgaaaaatgc ctgcagactg catccagcga
    27241 tgatttggca tcaaaactga aaaccgccgg atattcacgc agttttattc agtattcatc
    27301 gggtaataaa tacgctgcgt tatctgcatt tggccgggca ttcacggtta atttcaatgg
    27361 cagtaatacc gcgattacgc tcaagtttaa gcaggagccg ggtgtcgggt atgaaacact
    27421 gacagtcagc caggcatcgg cacttgatgc aaaaaactgc aatgtgttcg tgtactacca
    27481 gaatgataca gctatcctcc agcagggagt gatggctaac ggcgatttct ttgatgaacg
    27541 ccacggcctg gactggttac agaattatgt gcagaccaac ctctataacc tgctttatac
    27601 cagcaccacg aaagttcccc agactgaagc cggtattacc cgactgttat caaatgttga
    27661 aaaatcactg gatcaggccg ttcagaatgg actgattgct ccgggcgtat ggaacggggg
    27721 cgaccttggc cagttgtcat caggtgacac gctgcccaaa ggttattacg tatacgccca
    27781 gccgctggat gaacaggcac aatcagaacg tgaagcccgt aaggctccgg tgattcaggc
    27841 tgcaataaaa cttgcaggcg cggttcatta cgctgacgta cagattaacg ttgttcgcta
    27901 aggggaagtg aatgtctacc tattctttta tggatgtcac tgcgacgctg accgggccga
    27961 ccggttcgat tgacctcggg tacggttctg caagttctga agaggggatt gtggttgcga
    28021 tgggcggccc taaaaacacc atgaccatcg gttctgatgg cgaagtgatg cacagtctcc
    28081 atgcagataa aagcgggacg attaccgtta accttctgaa gacatcaccg acaaataaaa
    28141 aattgtcgct ggcgtataac gcacagagcc agtcttctgc cacatggggg aataacgtta
    28201 ttgtgatccg aaacaaggtc agcggcgaca tcatcacggc acgcagtgtt gcgttccaga
    28261 aacaaccgga taatgccaac gctaaaaccg gtaatacgat gccgtgggtg tttgactgcg
    28321 gcaagattga ccaggttctc ggggagtttt aatacatgga attcgaaatc aaaggcgtga
    28381 aatatcgcgc ggcaaaactc agcgtttttg atcagctgaa agtgacccgc aaacttctgc
    28441 cggtgctggc aggaatgatg tcagatttcg ggagcattcg ctcccgtttg cctgctgatg
    28501 gcaaaatcga caccgtgaaa ttcgagcagt taaaaccggt gtttgaaacc atgctcccgc
    28561 gtatcgctga ggaactgtct tccctgaccg aagatgacac cagtgcgatt attcatccct
    28621 gtcttgcggt ggtatcgcgg cgtcatatgg acggatgggt tccggtattt acccagggcg
    28681 aactgatgtt tgatgatatt gacttgctgg tcatgctgca tctggtggcg cgggtggtcg
    28741 ccgattcgct gggaaatttt ttgcctacac cccttaccag cacgacgcag agcctgcaac
    28801 agggctgacg tttaacagcc tgccggacgg gctgtcttac cttctcaatc cggttgacgc
    28861 cgggttaatt ccttatacag cacttaaaga tggctctgtc gatttgtacg acattgctct
    28921 cttgaatgac catctggcgg taaaagcgga taaccagcgg cgcattgaga aatggagaga
    28981 ggataatgaa cgctgaaact attaaagatt tcctcgtctc gcttggcttc agcgtggatg
    29041 atgcaggagc gaaaaagttc ggttctgtcc tcgccggtac aactgcaaat gtcatcaaaa
    29101 tgggactggc cgttgaagga gctgcgctgt ccgtggtggc cttcacggct aagatcgcct
    29161 ccggcctgga taatctttac cgggcgtcac agcgcaccgg cgcgacagtc cagggaattc
    29221 agtctattgg ctatgcggtt tcgcaggttg gcggcagcgt ggacgcggcg cgaacctctc
    29281 tggaaagcct ctcccggttt gttcgtaaca atcccggcgc ggaaggcttc ctgaatcgcc
    29341 tgggggtaca gacacgggat gccagcggta acatgcgtga catggccgct atttttacag
    29401 gtgtaggcca gaagctcagc ggcatgccgt attaccgggc taaccagtat gcgcagatgc
    29461 taggcattga cgaaaatacc cttatggcga tgcgccgggg tgtgggtgat ttctccgggc
    29521 agtacagcgc aatggcgaaa gctatcggct tcaatgctga cgaggcggcc agaagctcca
    29581 acaaattcat gacctccctg cgcgagttcg gcgcgatggc aggcatggcc cgtgacaaaa
    29641 tcggctctaa tcttgctggt ggtctggcgg gttcgctgga cacactgcgc cgccacatcc
    29701 tcgataactt cccgcgcatc gagcagaccc tgacgaaagc cataaaaggc attctggcga
    29761 tcggagacat catcgggcgg ctgttcttca ggctaattga gggaacatcc agccttatca
    29821 cctggtggca atcgctggat aagcaaacgc gggagctcat ctcgctgttt ggcgcgctga
    29881 cgattgcgct gcgcattctg aacagtacgt tctggatgtc gccgattggc ctcattaccg
    29941 cgctggcggc ggggattgcc cttctgtggg aggactatca gacctggaag gaaggcggcg
    30001 acagcctgat tgactggggc aagtggaagc cggaggtcga tgccgcgctg aagatggttc
    30061 gtgacctgaa agggtctgtt aatgaactgg cgaaagcgct ggcgaaactg ctcaatattg
    30121 accccaaatc atggtccctg aagtgggatt tcagcaactt catcgaccag atgggcgaat
    30181 tcagcaaaat gctgaacatg atcgccgacc tgctcaacgc tatcaaagat ggccgctggg
    30241 ctgatgccgt catcatcggc aaacagatac ttaatcaggg cagcgaaaat ccgtcagcga
    30301 tgccgatggt tacagacagc gctaacagta ctgccgactg gattaaagag cactggggat
    30361 tcgatccccg cagtgtgggc cggacggtac gcggctggtt tggtgatgat gagccggaac
    30421 aatatgcaca ggctacgaaa cgaggagaac ggaataacaa tccgggaaac cttaattttg
    30481 ctggtcaggc aggggcttct cttgaacgcc cgggcgggcg atttgccaga tttgaaactg
    30541 cttttgatgg attacgggct cttgctcgtc agttaatgct gtacgccgga cggggaataa
    30601 acagtgtgga gaaaattatc tctacctggg cacctgcgtc tgataataac aacacaactg
    30661 cgtatatcag ggctgtatcg caacgactgg gagtggatcc ccgggctgcc ctgaatatga
    30721 gcgatccgca aaccatgtca gcattgatga gcagcattat ccagcatgag aatggaagaa
    30781 atatctattc tcgagagctg attaataagg ctgccgtggc gggaattagt ggcaaaatga
    30841 cagaggttaa ccagcaaaat acttaccaca tttacggtgg cggagatccg cacgctgtcg
    30901 gtaatgaggt tgcacgtcgg caacagtctg caaatgctca ggtcatgcga agtaatcagg
    30961 tgagggtggg ttagtggata ttctctctac actttttcat cagcagagca gaaaaatagg
    31021 aatgattgtt cccagtgttg ttatttcaga gaagcataca gatatgcttg aaataacaga
    31081 gcatccggta gaggtcgggg ccgctgtcgc tgatcatgcc tataaaaaac cgtcagaagt
    31141 ggtgatggag gttggtttcg ccggtggcgg cgcattgctg gattttgcca gtaatctgac
    31201 ggctaccagc ctgctcggcc tgagtcctca gcagacgtat caggagctac tggatctgca
    31261 ggaaagccgt atccccttcg atgtggtaac cggtaaacgg ctgtacagca acatgttgat
    31321 ccgggcgctg gaagtgacga cggacaagac aaccgaaaac gtcctgtccg ccgtcctcac
    31381 cctgagggag gtcattatct cccggacaca gcagattacc gtcgcggata aaaccaacat
    31441 gaaggaaggg gccagcacgt cggcggtaca gaatagcggt aacaaaacca caaagcctcc
    31501 agatacttca ctgctgaaaa gcatcacggg taacgtggcg tcattactgg gaggcggcta
    31561 atgacaattc aggaaattcc gctgacagcg gacaaccagc agttcagcat cgtcctgggt
    31621 ggtgtcacct ggcggattag catcatatgg cgcgatcctt actggattat ggacctgcag
    31681 aacgacagag gagagccggt aatctccggt attcctctcg tcactggcgc tgatctgctg
    31741 gcgcagtacg cctgtatggg acttggtttt aagctgttgg tggtctgcga tgacaacaca
    31801 caggattatc ccacgaaaac tgacctgggc gggcgcagtc atttgctggt atcaacggag
    31861 taagcatgtc acagaactgg atgagacatt tcgagctgca gcttgtggac gggaacggta
    31921 agggaattga gctaagtgat ttcaaagtca cctttacgat cgactggttc aacatcagca
    31981 gcgcgtcccg ggtagggact atcaaaattt ataacctttc ggcagatact gtgaaccgaa
    32041 ttaccgggca ggagttttcg aaagtgcggc tgattgccgg ttacgacggt atcgcgccgg
    32101 aggtggcggc aagcgacgtc gggactgtgc gtgaagttga cgcggcggac gtgggccaga
    32161 gtgatggccg caactacggg ctgattttca gcggagaaat tcgctactcg gtcacaggaa
    32221 aagacagtcc ggttgatacc tacgtcctga ttcaggcagc agatactgat ctggcatttg
    32281 ccaccagtat aacctcacag acgctggctg ccggttacac agtcgcagat gtgaaccgtg
    32341 cgctgatgaa agactttgag gccaaaggcg cgaccgaagg actgacgcct gaaatgcctg
    32401 ctaccgtata ccctcgaggg cgggtgctgt tcggcatgac acggcatctt atggataacg
    32461 tggccggaca atgtggcgca acatggcaat ttgtggacgg tcagcgccag atggtggcga
    32521 ataacgagta tgttcacgac gcgattgtgc tcaacagcgc caccgggctt atcggcatgc
    32581 cgcagcagac tatcggtaac ggcgtaaacg tccgcgctct tattaatccg aacatccggg
    32641 ttaacgggct tattcagctg gatcaggctt ccgtgtaccg caccgcgctg tcgaacaatg
    32701 atatcgcgat ggctggtggg cagatcaccg accagaacac agacggaaat atcacgctca
    32761 gcggcaccac agcacaacct gccagcatcg caacggatgg cgtttatatt gtgcgcggga
    32821 ttatgtacac tggcgataca aggggccagg cgtggtacat ggatatgatg tgcgaagcgc
    32881 gtggcgcggc ggatctgtat acgcaatcgg ctttgcaaag gtgatgagca atgaggggta
    32941 tcatttttct gttagctgtc ttttctgcgt gcagcgtgtg ggcggatggc ttcacggtta
    33001 aatgcggtgg ctacactatg gttgcaaacc agggcgagtt atcgacaatt aacggtgaaa
    33061 gagttacctc tcaaaaaatc accgaactgg gtaccaatgg tttgaaagta gacatgggga
    33121 ttatgcctgc caaagacggt aacaactacg gctttgaata cattcgtcgc cctggtaccg
    33181 aaacgcgatt cctgaatgtg caactgctgc agaacagcat ggatgcaccg aaaatcatcg
    33241 gatctttccc gtgtaaaaag gttgatggtt aacccaaagt tacatttctg catagtgttg
    33301 agtttgttca cattcactaa ataatgattt tttatcgctt gctaggtggt tgcatgatca
    33361 ctaaactttg cgaactttta gcgctcagct aactttgaat aagtggtgta tagtcgttta
    33421 aaacgacaga gggattgggt atggcgatta gctacgcatt tgcgctggca acaattacac
    33481 aacaaatgaa tcaggttcag gaagctgtta acggtgcttt caaacccctg atctctaatg
    33541 cttgtgaact gccacaacga ttagatgctg aagaggtatt tcgtcgttgt accgcgattg
    33601 ctgcacgcgc tcaagaaatc gaaaataccg cgaaggaagg catgtctcat ctcgaagctt
    33661 ttcgaaatgg gagaatcatt gttgatgagc ttccggaaga gtttttatcc catctcgaag
    33721 gtcttgccaa ggcatgccga aatgctaaag gacatttagt ggatatgttc tctgaagcgg
    33781 aaaggtcccc tatgtggcaa ggccatctgc aaatgttgcg cccattaaaa cgcaaatatg
    33841 ttcgtgcgtt gaccgccgtc gagaacactg caactcagtt ggccgctgaa gttaggcaat
    33901 cgcagccttt ccaggctgaa ctcttgtcag ataatgttac ccgtgaagag gctattgaac
    33961 tgatctcaac atcgcataag atgctgggag ccaacgccca taaatggatg tgacatggca
    34021 aaagtcagta tcacgggaga attgcgtcat ttagctgcag cacataagta tgctcaaatg
    34081 cttgccgatt atatttcaaa gggttcccag ttctggtgtt ttggttcgtt aggcggtttt
    34141 gaacgtaact atgatgcgat ggctgccaac atcaggaaaa ttcacttaaa gctacctggc
    34201 gataaaccct ggcctccaga agcatctctg agtgaacgga catgtgataa ttttttggta
    34261 tatgctcagc atctttatat cgatgaacac tatcagatat tggcaatcat cagcccaaac
    34321 gctcatcagc aagctgattc gatgcttccc cggctgataa aattagcaga ggaaaccttc
    34381 atagaacttc ctcctgatga actcgaaaaa ttgaaaacct acgattcata aacccgccac
    34441 ccggcgggtt ttttgctttc tggagcctac taaatggcag tatctgacca gacccgcagc
    34501 ggcgaccttg ccgaaacatt caaatctgaa cgggaaacaa caaagaatca gatccgtgtc
    34561 gccttgcctg gcattattca gtcattcgat cctgatgcgg tgacggcggt tgtgcagcct
    34621 gctatccgtt cggttgaaaa ggataatgac ggcaaccgca ttaccaaaaa ttacccattg
    34681 ttggtggatg ttccagtggt attcccacgt ggcggaggat gtacgctaac gtttccgatt
    34741 aaagccgggg atgagtgtct tgtcgttttt gccgatcgtt gtattgattt ctggtggcag
    34801 agcggcggaa tacaggaacc ggtcgatgac agaatgcatg atttatcgga tgcgttttgt
    34861 attgtaggcc cccagtcgca ggcgaggaag attagcggca ttaataccag tgccacacag
    34921 ttgcgtagtg acgatggcag cacctatttt gagcttaatc ctgataccag gaaaattaaa
    34981 attgtcgctc cgggtggtct tgatgtggtt gcccctctgg ctgatttttc tgagaaagta
    35041 accattcatg gcctgttaac ctggatgggg ggcatggtgg ggtctgttgt ttctggtgtg
    35101 gcttcaaaaa tcactggtgt tgttgagttt ttgggtagcg tgaaggctaa cggcaagcca
    35161 atcgatgata cgcacactca tggcggtgtt cagcgcggtg gaagcaatac cgatggggta
    35221 aactgatgcg atacagacgt gaagacgccg atggcgatta cacctttggc agcggtgatg
    35281 acacctggct gattaactca ccggaggccg tggcgcaggc ggtaaaaacg cgattcgaat
    35341 tgtggtatgg gcaatggttt ctcgatacca ccgaagggac tccgtggatc cagtccgtac
    35401 tcggtaagca gaagccggaa acctacaacc tggcgatccg taagcgcatc ctcgaaacgc
    35461 ggggcgttaa atcaatcctc tctttcaata cgacggtgga taccacgacc cgacgtgtca
    35521 tgttttccgc tgaaatcgac actctttatg gaataacgac tgttacatcg gaggcgtaat
    35581 ggctctgaac cttgattctc ttggtttatc tgcaaaggta accgcagagg ggatcagtgc
    35641 gcctgattat cagacgatac tcagcaccct gattagctat tttcagcaga tttatggcag
    35701 tgatgcctac ctcgaaccgg acagcaaaga cggccagatg gtggctctga tggcgctggc
    35761 gattcatgat gccaataata tggcgataac tgtctacaac tgtttttcac cggcaaccgg
    35821 ctatggggct gcactgacca gcaacgtgaa aataaatggt atttcacgta aaggcgcgac
    35881 gaactctacg gttgatttgc ttcttacagg aactgcagga acaaccatca ttaatggcag
    35941 cgtgaaagac agtaataatg tgatatggcg tttgcctgct tcagtggtgg tcggcgtgga
    36001 tggtacagtg atggcgaccg caacatgttc cgtcagtggt gcagtggcgg cgctggctgg
    36061 aactatcact gaaattaata cgccaacccg tggctgggtt tcggtaacta atcctgctgc
    36121 ggctactgtt ggctctccgg cagaaactga tgctgagtta cgtatccgcc agtcgcaaag
    36181 tgttgcgttg ccatcgataa ccccatttga agcactggat ggtgctgttt ctaatgttac
    36241 cggtgtaacc cgccacaaac tctatgaaaa cgatactggt tcggaggacg gtaacgggtt
    36301 accgccacac tctgttgctg taattgtgga tggcggtgat gtgacggata ttgctcaggc
    36361 tatcagagga aataaaggcc aggggacagc cactcacggt acaacatccg ttacggttcc
    36421 ggataaatac ggcaatcccc atgtaatcaa attctcgcgt tccagtgatg tgcctgttta
    36481 tgcccggatt aaattaaaag tttttacggg ttatacctca cagatagggc agcagatcca
    36541 gcaggctatt tccgactata tcaatagtct gacgattggt gattcagtcc ttttaagtcg
    36601 catttactca ccggcgaatc ttggcgtggt gagtggcggg aatgcacgct attacgatat
    36661 tcaggaactg acgattggga aatccccggg ggctttgtcg tcatcaaaca ttgatatcag
    36721 atacaacgaa tctgcgtcct gtaccccaga aaatatcgtt ataacggtgg agtcatgagc
    36781 aaatacaccg aactaatcac gaactaccac gccaccaaac ctaaatttct tgcgcatgtt
    36841 gatctgatga cccggccact tattgatgtt gcggctgcca ccagagggct gattactgca
    36901 tttgatattg actctgcggt tggtgtgcaa cttgacattc ttggattgtg gatcggacgc
    36961 agccgtgttg tcagccagcc tatctcaggt gtctatttca gctgggatac cgacgggctt
    37021 ggatatgatc agggtgtatg gcaggggcca tacgatcctg attccggata catgtacctc
    37081 agcgatgaaa cttatcgtgt catccttaaa gcgaagattg cgattaataa ctgggacgga
    37141 cggaatgatt cgcttccggc aattcttgac gcggcgacag caggatccgg actgcgaatg
    37201 cagatagtcg ataaccagga catgacgata tcggtctggc tctttcctga tactgatatt
    37261 tcagatgtat cgcgtgagtt aattgcggca attaaacagg gatatctcac agtaaaagcc
    37321 gccggggttt gggcgggtgg cattgaaaca ccttcggtgg aaactccatc ggaaggttca
    37381 aaattttttg gttttgatat ggataacgaa ttcatcagtg gttttgatgt aggggcatgg
    37441 ggagtattac tctgatggcg aaaaatgact ttaaagcgtt tgcaactgat cggaatgcca
    37501 atgttatgtc gcaggaggaa tgggaagcgt tgcctgcgct tttatccgga tttacagcag
    37561 ggaaagcatc cagtgcgcaa gtcaataagg ttattcggca ggccagcttt attgctgcag
    37621 ctctggccca gtttgtaagt gacaaaacgc aacgggatgt gcttgataat ggtgatctgc
    37681 ccggttttgt tgaattgctg ggatcggggt ttgctgttga atacctgagc cgcaagaatc
    37741 cgtttggcga tatcaaatcg gatggcacgg tgaaaacggc tctcgaaaac cttggtttag
    37801 gagatatcag tgctacaggt gttgcgacag gtagtatggg cgaaaccgga tatgccatat
    37861 ttccgatgat gattggcggt acgcgtaaga cgcttattat gcaatgggga gtaatgacat
    37921 ctctgagatc aggcggcact atggatatta atctgccgat tgcttttcct actatggtta
    37981 tgcaggcata tgcaacacat gataatgtga tacaaaccgc acgaccgaca gtatatgcaa
    38041 ttgccccaac aagccgaacc actatcactg cgacggcgat gagcttacgt gctgactcag
    38101 tgggtgctgc agtgacgcgc agtagtgaca gtgatttgtg ttacggccgt tatcttgcaa
    38161 tagggtactg aaatgaatct gaaatatttt tctccatcag aaaatgcgtt ttatgccggg
    38221 gagcttaagg aaagctatat tgctgcagac tcatggcctt ctgatgcctt ggaggttgac
    38281 gaaagtcttt atcttgagtt tatcgatgag ccgccagcag gtaagtatcg tggagtggat
    38341 gatgcaggta tgccctgctg gcttgaaatt cctccaccca ccgcagagga aatggcatcg
    38401 aatgcagaga atatgagaca gcagctcatt gatgaggcaa atgtttatat aaacaaacgt
    38461 cagtggccgg gaaaagcagc gataggccgt ttaaaggcgg atgaactgga acgatataac
    38521 ctttggctgg attacctgga tgcgctggaa gtagtagaca cctccagcgc gccaaacatt
    38581 aactggcctg atcctccgga tgcgtaggcc atacgggttt tgctgtatcg acacgcatta
    38641 gcaagacccg atatttcttc cattcggcca gagcgacggt ttcctcatcc gtcgcgatac
    38701 caacatcaac tgcatcctgc cgccaggcta tctcagcgtc agcaatagcg cgtaaactgc
    38761 tgggataaca gcccaatgga acgcttcttc aggagtctga agaacgaatg gatgccggtg
    38821 gtgggttacg taagcttcag cgaggcagct cacgccataa cggactatat cgttggatat
    38881 tacagcgcac taagaccgca cgaatataac ggtgggttac ccccaaacga atcggaaaat
    38941 cgatactgga aaaactctaa ctcggtggcc agtttttgtt gaccacttca cacgtcgtat
    39001 gcaagaacgt gctgcggctg gctggcgaac tttcgatagt gcgagtattg aatgatttcc
    39061 aactgttgcc gattttacta tgttttcagt agaacactta gacaaaactg aggcacacaa
    39121 atctttgcac tggattgcaa ggctttgtgc tcttcgatag tggttaagga ggattactcc
    39181 acctttccat caagccaatc cgcccaccac tgcatcattt ctctgcgctt atcgagatac
    39241 tgagcatggt tgtaaattcc acgcacagaa ccgccgttgg catgtgccag ttgcacttca
    39301 atagcgtcag caggccattc gtgctcgttc ataatcgtgc tgaattcatg cctgaatccg
    39361 tgaccgcttt ccagaccttc atagccgatt tgtttgatca caagcaatac cgcgttctcg
    39421 cagattggct tcttcttatc gttgcgcccg gcaaaaacaa actctgagac tggtttggtg
    39481 attgagctta gcgtagtgag aagttcaacc acctggtctg acatcgggac cacatgaatt
    39541 ttgcgtccct tcatcacact ggcgtcgatg gtgataatcc tgttttcaaa atcgacgttc
    39601 ttccatagca tggaacgaag ctctttcgtt cttagggctg tataacgtaa aacttttgtc
    39661 gcaatgagcg atacgatact tcctgaaaat gttgccagtg ctttgttgaa tgcaggcatc
    39721 tggtctgctg gaaggaacgg gaagttcttc ttgcggtatc ctttcatggc gtctgcaagg
    39781 tcaggtgctg ggttatattt agcccttccg gtgacaatag cgtaacgaaa aacctcgccg
    39841 catcttctgc gggctttgtt ggctcgctcc attgcaccgc gatcttcaaa tctgcggatt
    39901 acttccagca gttgcatcgg ctcaatatcc tgaatctcaa gaccgccgat gatgggtaaa
    39961 atgtcgtcat caaacatttt ggcaagttca gttgcatagc ctactgacca gacttgcttc
    40021 ttgtgctcgt accattcctt gtaaatcgca ctaaatgaat tgttgttaga cgaagccttt
    40081 ttcgccttta ccggatcgat gccaaccgag atgtctttcc tcgcagtcca tgccttatcc
    40141 cttgcttcct gcaaagtcat aagcggatat tttccgacag tcaggatttt ctccttaccg
    40201 tcaatcttgt agcgaagctg ccataccttt ttcccggata cagggacata aaggtacagg
    40261 ccattaccat cgagaaggcg gtatggtttt tctttcggct ttgctgcttc aatctgctta
    40321 acggtgagca tgggtaaaaa tccggtgggt aaaattattt tatccacttt ttacccgtca
    40381 tggagtgcgg ctgtcaacga tctgacgcga accatgacga accgtgaata tacggaagga
    40441 ttgatattca ggggattttg cggactagta cggatggtgg cgaactgata aatggtgtcc
    40501 cctgcaggaa tcgaacctgc aattagccct taggaggggc tcgttatatc catttaacta
    40561 agaggacaat gcggcatgag tatacccgct aatggactgc ggggtaagta cgctgccgct
    40621 cgattgctta aaccctcgcc atttatgctg ggtttttatc atttttctta atgttttccg
    40681 cacgttctgc tttttggcgt gcttctgctt tacgcttgtt gctcatgtcg ttacgaatct
    40741 gtgcatgact cattaacgcg aagataaagg tgccgccgca gatgttcccc gctaaagtag
    40801 gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt gccgttaaac accagataga
    40861 ggatttcaac agaaccgaca acgatgtggg tggtgtcacc cagtgcaata agccaggtca
    40921 tcaatataat caccacaatc tttgccgcac ccgcagcagg aaacatccaa accatagtgg
    40981 cgatcagcca gccggaaatg atcgcgttgg caaacatctc gctgggggtg ttcttcatca
    41041 catccatgcc gattttgaca aatgcatcgc gagtttcttc attgaagata ggcatatatt
    41101 caaatgccca tgcagcaata cctgtcccga gaatattacc cagcagcacg acgccccata
    41161 atcgcataag taagccgacg ttgctcattg tcggtttttg catgacgggt agtaccgcag
    41221 tcacggtgtt ttcggtaaat aattgctggc gggccataat gacgataata aaaccaaagg
    41281 tataaccgag attctccagt aagaagctgc ctggcactcc ttccagttcg acatgaaata
    41341 tcccttttgc cagtagcgaa gcgcccatcg acagacccgc cgcaatggct gaccacagta
    41401 gcgccattgc gtcgcgttcc agctcttttt caccatcctg gcggatatgc tcatgaattg
    41461 ccatcgcccg ggaggggagt cggtcttcat ctatttctat ttttttgccg cgctcttttt
    41521 cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat tttgtcgttg tccatggtct
    41581 cttctcttga attagcacgt atagctaaag cgtagcggct tttttgctcg caactggcgg
    41641 gagttactct gaaaatgtag aaaaggctgc gtttgccttt ttctgtttct atagaatcaa
    41701 gtagcctaca gggcggcgat taccaggcta tgatcaaatc agcaaatcag ggcgtctgga
    41761 catcagttga cgtgctgtta caatcgccca cacctaaaca ggcggatacg gtatcgttcc
    41821 gtcatggatg gcaaactgca taagccataa aaaaacaggg agacatttat gaagcttcgc
    41881 ctgtcggcgc ttgctctggg aactacgctt ctggtggggt gtgcgagttc cggtacagat
    41941 cagcaagggc gttctgaccc gttagaaggg ttcaaccgca ccatgtacaa cttcaacttc
    42001 aatgtattag acccgtatat tgttcgaccg gtcgctgtcg cctggcgtga ttatgttccg
    42061 caaccggcgc gtaacggttt gagcaacttt actggcaacc ttgaagaacc tgcggtgatg
    42121 gttaactact tcttgcaggg cgacccttat caggggatgg tccactttac ccgctttttc
    42181 ctgaacacca ttttggggat gggcggtttt attgatgttg cagggatggc gaacccgaaa
    42241 ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg gtcattatgg cgtgggttat
    42301 gggccttacg tccagttacc gttctacggt agcttcacgc tgcgtgatga cggtggtgat
    42361 atggcggatg gtctttaccc ggttctttcc tggctgacct ggccgatgtc tgtgggtaaa
    42421 tggacgcttg aagggataga aactcgtgcg cagttgttgg attccgatgg tctgctgcgt
    42481 cagtcgtccg atccttatat tatggtgcgc gaagcgtact tccagcgtca tgatttcatc
    42541 gctaatggcg gcgaactcaa accgcaggaa aatccgaacg cgcaagcgat tcaggatgat
    42601 ttaaaagata tcgattctga ataagaaaca aataaaaaag gtgagtcgca atactcacct
    42661 tttttgttgt tatccctcaa ttaccacaag taaaaggaga taaaccgaca atgacccgga
    42721 cgtaatgatg taaaaatact gatgttcgtc actgactatt tccgatattc aggagttgaa
    42781 aaatcaaaaa actggaaaaa taattttaaa tatcatcaaa attaacacct ggcgcagata
    42841 gttgattaat ttcattttga tattccgtct ggcgattttg aagccttttt tcaactttat
    42901 cttcgagatc cttaaagtct ttgtatttat tttcaggatg gtcaacgtca actttgttag
    42961 cgaataaagt agaaattatt tcttttacat tatcgcgtct aaattttgca tcgtccactt
    43021 gaaaattata tcttattgct cgacctaaac gtttaaaaac ttcgttaggt tctctcagtc
    43081 tttccccgtt gagctcgttt acaacctgat gatcggaacc tgttgcaaat gattctgcca
    43141 tgatagcttt gctgctttca aaccacgctg aagcactcct ggcgttttca ctacaatatt
    43201 gggtgagact atcaccatta atacgaattc ctctttgaat gtggtagtcg tcctgatcgt
    43261 agtaaccgag agataagtaa tcatttccgt ttttattttt aattacaata gcgagagagt
    43321 cgcctttttt atctttaccg taccaggtaa ataagtccgt tggttcgcta ttcccgctct
    43381 catcaaattc cgttaccgta tgtctgagtt tgtgtgatat cttatcgaag ataaaaccag
    43441 gatctccatc atatatttct tttaatgcgc tgaagatcat ttccttgtta ttgatatcat
    43501 gagtcttctc aactatgttg tcattaatga tagtacggac gagttctttg tctttatagt
    43561 tcgcaggtaa ttctattggt tcttggttgt cgataccaat agtaatatgg
//
LOCUS       DAPHDX010000024        43298 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPHDX010000024 DAPHDX010000000
VERSION     DAPHDX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.024"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            150..2789
                     /locus_tag="RH058_001288"
     CDS             150..2789
                     /locus_tag="RH058_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV3136895.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2786..2971
                     /locus_tag="RH058_001289"
     CDS             2786..2971
                     /locus_tag="RH058_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV3136896.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2976..3305
                     /locus_tag="RH058_001290"
     CDS             2976..3305
                     /locus_tag="RH058_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV3136897.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3477..4382)
                     /gene="feaR"
                     /locus_tag="RH058_001291"
     CDS             complement(3477..4382)
                     /gene="feaR"
                     /locus_tag="RH058_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDV3136898.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            4618..6117
                     /gene="feaB"
                     /locus_tag="RH058_001292"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             4618..6117
                     /gene="feaB"
                     /locus_tag="RH058_001292"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDV3136899.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGLNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDSWRETKSVCVRY"
     gene            complement(6175..8448)
                     /gene="tynA"
                     /locus_tag="RH058_001293"
     CDS             complement(6175..8448)
                     /gene="tynA"
                     /locus_tag="RH058_001293"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDV3136900.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8696..10741)
                     /gene="paaZ"
                     /locus_tag="RH058_001294"
     CDS             complement(8696..10741)
                     /gene="paaZ"
                     /locus_tag="RH058_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDV3136901.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            11026..11955
                     /gene="paaA"
                     /locus_tag="RH058_001295"
     CDS             11026..11955
                     /gene="paaA"
                     /locus_tag="RH058_001295"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDV3136902.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            11967..12254
                     /gene="paaB"
                     /locus_tag="RH058_001296"
     CDS             11967..12254
                     /gene="paaB"
                     /locus_tag="RH058_001296"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDV3136903.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            12263..13009
                     /gene="paaC"
                     /locus_tag="RH058_001297"
     CDS             12263..13009
                     /gene="paaC"
                     /locus_tag="RH058_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDV3136904.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            13024..13521
                     /gene="paaD"
                     /locus_tag="RH058_001298"
     CDS             13024..13521
                     /gene="paaD"
                     /locus_tag="RH058_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDV3136905.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            13529..14599
                     /gene="paaK"
                     /locus_tag="RH058_001299"
     CDS             13529..14599
                     /gene="paaK"
                     /locus_tag="RH058_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDV3136906.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            14596..15363
                     /locus_tag="RH058_001300"
     CDS             14596..15363
                     /locus_tag="RH058_001300"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDV3136907.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            15363..16151
                     /gene="paaB"
                     /locus_tag="RH058_001301"
     CDS             15363..16151
                     /gene="paaB"
                     /locus_tag="RH058_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDV3136908.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            16153..17580
                     /gene="paaC"
                     /locus_tag="RH058_001302"
     CDS             16153..17580
                     /gene="paaC"
                     /locus_tag="RH058_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDV3136909.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            17570..17992
                     /gene="paaI"
                     /locus_tag="RH058_001303"
     CDS             17570..17992
                     /gene="paaI"
                     /locus_tag="RH058_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDV3136910.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            17992..19197
                     /gene="paaJ"
                     /locus_tag="RH058_001304"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             17992..19197
                     /gene="paaJ"
                     /locus_tag="RH058_001304"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDV3136911.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            19224..20537
                     /gene="paaF"
                     /locus_tag="RH058_001305"
     CDS             19224..20537
                     /gene="paaF"
                     /locus_tag="RH058_001305"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDV3136912.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            20638..21588
                     /gene="paaX"
                     /locus_tag="RH058_001306"
     CDS             20638..21588
                     /gene="paaX"
                     /locus_tag="RH058_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDV3136913.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            21570..22160
                     /gene="paaY"
                     /locus_tag="RH058_001307"
     CDS             21570..22160
                     /gene="paaY"
                     /locus_tag="RH058_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV3136914.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            22391..23251
                     /gene="pdxI"
                     /locus_tag="RH058_001308"
     CDS             22391..23251
                     /gene="pdxI"
                     /locus_tag="RH058_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV3136915.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            23315..25621
                     /locus_tag="RH058_001309"
     CDS             23315..25621
                     /locus_tag="RH058_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDV3136916.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVNIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALTHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPVVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            25792..26397
                     /locus_tag="RH058_001310"
     CDS             25792..26397
                     /locus_tag="RH058_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV3136917.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMRAER"
     gene            26397..27293
                     /locus_tag="RH058_001311"
     CDS             26397..27293
                     /locus_tag="RH058_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV3136918.1"
                     /translation="MLEKSLATLFALLILATLINHFLIWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            27309..29066
                     /locus_tag="RH058_001312"
     CDS             27309..29066
                     /locus_tag="RH058_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV3136919.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            29056..30372
                     /locus_tag="RH058_001313"
     CDS             29056..30372
                     /locus_tag="RH058_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV3136920.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLKLWENR"
     gene            complement(30423..31028)
                     /gene="azoR"
                     /locus_tag="RH058_001314"
     CDS             complement(30423..31028)
                     /gene="azoR"
                     /locus_tag="RH058_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV3136921.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLITGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            31229..35131
                     /gene="hrpA"
                     /locus_tag="RH058_001315"
     CDS             31229..35131
                     /gene="hrpA"
                     /locus_tag="RH058_001315"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV3136922.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTALGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            35403..36203
                     /locus_tag="RH058_001316"
     CDS             35403..36203
                     /locus_tag="RH058_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV3136923.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            36400..37839
                     /gene="aldA"
                     /locus_tag="RH058_001317"
     CDS             36400..37839
                     /gene="aldA"
                     /locus_tag="RH058_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV3136924.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37881..38882)
                     /gene="gap"
                     /locus_tag="RH058_001318"
     CDS             complement(37881..38882)
                     /gene="gap"
                     /locus_tag="RH058_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV3136925.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            39071..39601
                     /gene="cybB"
                     /locus_tag="RH058_001319"
     CDS             39071..39601
                     /gene="cybB"
                     /locus_tag="RH058_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV3136926.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            39846..40019
                     /locus_tag="RH058_001320"
     CDS             39846..40019
                     /locus_tag="RH058_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136927.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(40091..40240)
                     /gene="hokB"
                     /locus_tag="RH058_001321"
     CDS             complement(40091..40240)
                     /gene="hokB"
                     /locus_tag="RH058_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV3136928.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            40639..42279
                     /gene="trg"
                     /locus_tag="RH058_001322"
     CDS             40639..42279
                     /gene="trg"
                     /locus_tag="RH058_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV3136929.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(42317..43240)
                     /locus_tag="RH058_001323"
     CDS             complement(42317..43240)
                     /locus_tag="RH058_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3136930.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT        10640 a        10245 c        11565 g        10848 t
ORIGIN      
        1 ttcaaatata attgaaagct atggcgatat tgaaaaattc atcaacaact atgcttagtg
       61 taggcgcaac cttcaactga acggttaaac atgccacaat acccgtattg aatgcttaat
      121 ttttcgctaa atcaggatat taactaccca tgctgggtaa atataaagcc gttctcgcgc
      181 tgttattact gattattctt gtgccgttga cgctgctgat gacgctcggg ctgtgggttc
      241 ccacgctggc gggcatctgg ctaccgctcg ggacacgtat tgcattagat gaaagcccac
      301 gcattacgcg taaaggttta atcattcccg atctccgtta tctggtggga gattgtcagc
      361 ttgcacatat caccaacgcc agcctttcac atcccagccg ctggttattg aacgtcggca
      421 cggtagaact tgattctgct tgtctggcga aattgccgca gacggagcaa tcgccagctg
      481 ctccaaaaac cctcgcgcag tggcagtcca tgctgcctaa cacctggatc aatatcgata
      541 aactgatttt ttctccctgg caggaatggc agggaaaact ctctctcgca ttaacctctg
      601 atatccagca actgcgttat cagggcgaaa aagttaaatt tcaaggccag ctgaaagggc
      661 aacaacttac agtcagcgaa ctggatgtcg tcgcgtttga aaatcagccg ccggttaaac
      721 tggtggggga atttactatg ccgctcgtgc cggatggact tcctgtaagt gggcatgcta
      781 ctgcgacgtt aaacttgccg caggaaccgt cactggtgga tgcagagctg gactggcagg
      841 aaaatagcgg gcaattgatt gtgctggcac gggataacgg cgatccgttg ctcgatttgc
      901 cgtggcaaat tactcgtcaa caattgaccg taagcgatgg tcgctggagc tggccgtatg
      961 caggttttcc tttgagtggc cgactgggtg tcaaagtcga caactggcag gcagggcttg
     1021 agaacgctct ggtcagcgga cgactgagtg tgctgaccca ggggcaagcg ggtaagggca
     1081 acgcggtgct taattttggc ccaggaaaat taagcatgga taacagtcag ctgcctctgc
     1141 agctgaccgg tgaagcgaaa caggcggacc tcattttata tgcccgttta cctgcgcagc
     1201 taagtggaag tctgactgac ccaacgctgg cctttgagcc aggcgcgtta cttcgttcaa
     1261 agggaagagt catcgattcg ctggacatcg atgaaatccg ctggccttta gcgggtgtaa
     1321 aagtcaccca acgtggtgtt gacggacgtc tgcaggccat tttgcaggcg catgaaaatg
     1381 aactgggcga tttcgtgctg catatggatg gtctggcgaa tgattttctc cctgacgctg
     1441 gccgctggca gtggcgctac tggggaaaag ggagttttac accgatgaat gccacctggg
     1501 atgtcgcagg aaaaggtgag tggcatgaca gcacgattac gctgaccgat ctctccaccg
     1561 gtttcgacca gttacaatac ggtacgatga cggtagaaaa gccgcgatta attctcgaca
     1621 agcccgtcgt ctgggtacgt gacgcacagc atccctcctt cagcggcgcg ctgtcactgg
     1681 acgccgggca aacgctgttc actggcggca gtgtgttacc gccatcaacc ttaaaattta
     1741 gcgtcgatgg gcgcgatccg acgtatttcc tctttaaagg cgatttacat gctggtgaga
     1801 ttggcccggt tcgggtaaat ggtcgctggg acggtattcg tctgcgcggt aacgcctggt
     1861 ggccaaaaca atcactgact gtattccagc cgctggtgcc acccgactgg aagatgaact
     1921 tacgcgatgg tgaactgtat gctcaggttg cattttctgc tgcgcctgaa caaggattcc
     1981 gcgcgggagg acacggcgtg ttgaaaggcg gtagtgcctg gatgccagat aatcaggtta
     2041 acggtgtcga ttttgtcctg cctttccgtt ttgccgatgg agcctggcat ctggggactc
     2101 gcggccccgt tacgttgcga attgccgaag tgattaatct ggtgacagcg aaaaatatta
     2161 cggctgattt gcaagggcgt tatccgtgga ctgaagaaga acccttgctg ttgactgatg
     2221 ttagcgtcga tgtgttaggc ggtaacgtac tgatgaaaca attacgtatg ccgcaacatg
     2281 acccggcgct gttgcggctg aataatctct catccagcga actggttagc gccgtcaatc
     2341 cgaaacaatt cgccatgtcc ggggcattta gtggtgcatt gccgttatgg ctaaacaacg
     2401 aaaaatggat agtgaaagat ggttggctgg caaatagcgg gccaatgacc ttgcggctgg
     2461 ataaagacac cgcagatgcg gtggtgaaag acaatatgac tgcgggttca gcaattaact
     2521 ggttgcgcta tatggaaatt agccggtcat cgacaaaaat taacttagat aatctcggtt
     2581 tattaaccat gcaggccaac attacaggta ccagtcgcgt tgatggtaaa agcggtacgg
     2641 taaaccttaa ttactatcat gaagagaata tttttacgct gtggcgcagt ttacgctttg
     2701 gcgataatct ccaggcatgg ttggagcaga acgcacgtct gccgggaaat gactgtccgc
     2761 aaggaaaaga gtgtgaggaa aaacaatgaa aattttactg gctgcgttga cgtcatcttt
     2821 tatgctggct gcctgtacgc cgcgtattga agtcgctgcg cccaaggaac cgatcactat
     2881 caatatgaac gttaaaattg agcatgagat catcatcaag gcagacaaag atgtcgaaga
     2941 gctgcttgaa actcgtagcg atcttttctg aggcgatgat gaagaaaaca ttacttcttt
     3001 gtgcgtttct tgttgggctg gtaagcagca atgtaatggc attgactctg gatgaagcca
     3061 gaactcaggg gcgggtaggt gaaacatttt atggttatct ggttgcgctg aaaacggatg
     3121 ctgaaacaga gaaattagta accgacatta atgccgaacg taaagcgagt taccaacaac
     3181 tggcaaagca aaataatgtg tctgtagatg atatcgcgaa actcgccggg caaaagctcg
     3241 tagctcgggc caaaccgggg gaatatgtac aagggataaa cggtaaatgg gtgcgaaaat
     3301 tttaatagca aaaccgcaat atcgggataa cgctacagga gaaagcgatt gatctatttt
     3361 cctgaaacaa ggtgaatatt caaaaactcc tgtcaaattg cctttcgccc tgaaaaatgc
     3421 atagtcaaat atctgtttta actaattggc gttgcagtac acacaacgcc aattagttag
     3481 cggaatttac gtcgatactc gcctggcgtc atcccaaagc gttgcttaaa taccgttgaa
     3541 aaatgactct ggtcagaaaa tccccaatga aagccgatgc ctgccagttt ttcatcatct
     3601 gcggcatggc gaatcgcatc tgcacaaaaa tcgagacgac ggttacgaat atattgcgcg
     3661 actaccaaac ctttatcggc aaacattcgg tacaaactac gtactgacat acctgtctct
     3721 ccggctatcc actccgggcg taatatctct tcgcgaatat tatcgtctat caacgtaacc
     3781 actttttgaa actgacgttc acgacgaggt tgaacagatt cccgctgatg aagtaccggg
     3841 cgcagcagac acaccatcgc ctgtagcgca gcttcacttt ctgtttcaga aagtgccgga
     3901 ttattcatgc tctcctgtaa caggcgatga ctgagttgca ccatgggtaa gtcagcgtcc
     3961 agtctttctg cgcagacagg tttttgatgg ggaaaatatt gttccagcag agtgcgtggc
     4021 aaaagtaatg aaatctgttt agaagactcc tgccagtaaa gcgaacaggg gcgtgaggca
     4081 tcgagtaacg taatatcgcc agcgccaatc tgcacctgac gctcatcctg ctccattatt
     4141 gcctgaccac taagctgaaa aacggtgtaa aaccaggcat cgtcgctgcc ttttacctcc
     4201 tgccaggtgc ggtataaatt cactccgttg gttgtcacgg tgctcagctt tagtcctttg
     4261 gcaaaatgcg tttccagtac acccgtgtaa cgctcagtca gcaggcgtcc ggtaaaattt
     4321 ccgcatacct gattgatttg ggaaagccat tgctgaaact cattatccac tgcggggttc
     4381 atggcacgtt ttcgctctgt gaaatgtatt tttattgttg catttgtgtt gcaataaacg
     4441 aagctaatga gcctgactat aggaaataag tcttgtcagg catagagaca taagcggtta
     4501 ttgtcacgat ttgcggagct tgtcacagct gacaaagcga atgtcacagc gaaaaaagtg
     4561 acttttcttg tcgctgcgta cactgaaatc acactgggta aataataagg aaaagtgatg
     4621 acagagccgc atgtagcagt attaagccag gtccaacagt ttctcgatcg tcaacacggt
     4681 ctttatattg atggtcgtcc tggccccgca caaagtgaaa aacggttggc gatctttgat
     4741 ccggccaccg ggcaagaaat tgcatcaact gctgatgcca acgaagcgga tgtagataac
     4801 gcagtcatgt ctgcctggcg ggcctttgtc tcgcgtcgct gggccgggcg attacccgca
     4861 gagcgtgaac gtattctgct acgttttgct gatctggtgg agcagcacag tgaggagctg
     4921 gcgcaactgg aaaccctgga gcaaggcaag tcaattgcca tttctcgtgc ttttgaagtg
     4981 ggctgtacgc tgaactggat gcgttatacc gccgggttaa cgaccaaaat cgcgggtaag
     5041 acgctggact tgtcgattcc tttaccccag ggggcgcgtt atcaggcctg gacgcgtaaa
     5101 gagccggttg gcgtggtagc gggaattgtg ccatggaact ttccgttgat gattggtatg
     5161 tggaaggtga tgccagcact ggcagcaggc tgttcaatcg tgattaagcc ttcggaaacc
     5221 acgccactga cgatgttgcg cgtggcggag ctggccagcg aagctggtat tcctgatggc
     5281 gtttttaatg tggtcaccgg gtcaggtgct gtatgcggcg cggccctgac gtcacatcct
     5341 catgttgcga aaatcagttt taccggttca accgcgacgg gaaaaggtat tgccagaact
     5401 gctgctgatc gcttaacgcg tgtaacgctg gaactgggcg gtaaaaaccc ggcaattgta
     5461 ttaaaagatg ctgatccgca atgggttatt gaaggcttga tgaccggaag cttcctgaat
     5521 caggggcaag tatgcgccgc cagttcgcga atttatattg aagcgccgtt gtttgacacg
     5581 ctggttagtg gatttgagca ggcggtaaaa tcgttgcaag tgggaccggg gatgtcacct
     5641 gttgcacaga ttaacccttt ggtttctcgt gcgcactgcg acaaagtgtg ttcattcctc
     5701 gacgatgcgc aggcacagca agcagagctg attcgcgggt tgaatggacc agccggagag
     5761 gggtattatg ttgcgccaac gctggtggta aatcccgatg ctaaattgcg tttaactcgt
     5821 gaagaggtgt ttggtccggt ggtaaacctg gtgcgggtag cggatggaga agaggcgtta
     5881 caactggcaa acgacacgga atatggctta actgccagtg tctggacgca aaatctctcc
     5941 caggctctgg aatatagcga tcgcttacag gcagggacgg tgtgggtaaa cagccatacc
     6001 ttaattgacg ctaacttacc gtttggtggg atgaagcagt caggaacggg ccgtgatttt
     6061 ggccccgact ggctggacag ttggcgtgaa actaagtcgg tgtgtgtacg gtattaatct
     6121 ggttcgctca taagtaaaaa acggcacctg gtgccgtttt tttgtctgaa acaatcactt
     6181 atctttcttc agcgccccta gcgttggcgt ttcgtcaaag aagttccatg gtttcagcag
     6241 agtatgtacc cattcggtcg gcataatcgg ccactcttcg gcgcgggcca catgtgtggt
     6301 gccggtggtc atccagacaa cggcgtcggt gttgtccagc gactcgttat ctttactgta
     6361 ttgtccaaga ccggtgtcat gagtagaacg gttcggatat ttgccttccg ggaaacgctc
     6421 gccaggatga taacgcgtta cccagagctg cttgtccata aagcttaaac gatgatagat
     6481 ccactcgtcc ggcgcgaact gggcaccttt tgctaccggg tgagtaccac ctgcataagg
     6541 aataatttga taggaaaccg gattgcccat gcggttctct ttgttcgggt tactcaacag
     6601 acgaatcgtg cccggatcaa atttctgtgc ggcatcctgt tcattgccga tgttgtactg
     6661 attaacttgc atggtactgg tgcgtgggcc accggcagta ttcggtttta ccactgggtc
     6721 catcgccacc aggctgttat tctcgccatc tacatccaga tcgaggcgga aattataaat
     6781 atgttggtgt gtagtaccca cgatattgtg atcgataagc gtgccgtagc gcgtgtcatc
     6841 tttcgccgtc tcatcgtgca tggttttcgc tttaacacct ttcaccgctt cgatgcccgt
     6901 agcaccggca tcaatgccaa tagtgccgtt ttcatggaag atccagtcaa aaatgtagtc
     6961 atagttaccc actgtactga tccagcgcac cactaactcc cggcgttcgg tactgacgtt
     7021 gggctggccc atttcctgat gcttatactc aggcccggca taacgttcaa ataccgcgat
     7081 agcgcgaggg atctccatcg gcacgccagt gtagtcggcg atggtttcat taaggagcac
     7141 tgcgttagac ggggcatctt taccacgagc aattggtgag gttagcgtgc ccataccgta
     7201 gtcaccagag tccagatacg ctttaaagta ccagccaata tcaggatcac cgtaaggcac
     7261 aatcatgccg ccgagagaac cttcgtacat gactttgcgt ttggtgccat tgtcgttata
     7321 agtcacggtg gagatcatcg gcccgacgcg agagttcatg ctgaggtgaa aatcccagtt
     7381 ccgccagtga atcatatcgc cagtaatggt gtaattttta ccttcaggct caatgatttg
     7441 cataggctta actgccggag caacgcggtc acggccatca aatgggcgtg cggtcattgg
     7501 caccggaact accggacctt cttcaatctt aacgattttt ttctgttcta aatcaacgac
     7561 cgccaccagg ttttcgatgg gatgtgccca gtagttgcca tcaccgacat caagatagct
     7621 gatgactttg agcaaccggg catcttgttt caggccatct ttaccatcga aataacctac
     7681 ggtcagcggc gtggtaatca cttttttcgc atcagtaata ccgcgtttct tcacggcagc
     7741 ggcaaattct tcactgttgt taataatgtt ctgcacactg gcgaaatcat ccagcaacac
     7801 cataccgtgg gcgtctttaa tgggttgcca ggagagcagt ttgttgtttt gcagatccac
     7861 caccgcttcg atgatatgtt tgccgtcgag cataatgacg tcggctttgc gcggctggtc
     7921 aaccggtttg ttttccagcg caaacgccca gacagcttct ttatctggcg gtagcaggga
     7981 gatctcagta aaacgggtat tgggtttgaa gtccgcggaa gctttaacaa tttcaacggc
     8041 ctgtttaatt tcgtccgcag ttagcgcatt aagtgggtga gggcgctttt ctacctgaaa
     8101 ggtttgatcc agaccggact ggaaaacatc gttaataaag gtgtcagaaa cccaggcctt
     8161 attgtctttc atcactaccg gcacttgcag tgccagaggt tgaccattaa caattgctgt
     8221 ttgcgcacca ggcttcactt tcacgtacgc gccatcttta atcagggtaa agatctgggc
     8281 gtagtcgtcc cactgcacat cggcaccaaa ttctttaagc gtttcatcca ttggtaccat
     8341 atgcgcttca ccaccgtggg caaataccgg cgcttgccag gcgaaactta aggcgactgc
     8401 caacgccagg gctgttttac gggcagaata cagagagggg cttcccatta ttaacctcgt
     8461 cagatgttgt gttcttgtta gcaaccgcgc tctgtgggcg gtttggtcag gttcacatta
     8521 tcagtactga tgtaaagggg attgcctgca cctgccaggt tgtttggcag gtgtgccagc
     8581 ttttcataca gtggatgccc tgaaaataga tgtacacatc atgcataatg tgacaacgtc
     8641 acaaaattta gtgaaataaa agggcaacta ttcgccgttg cccttcattc accgattaat
     8701 cgacaaaatc accgtgctgc ctggccacca gcgtcagaat tgaatacagc gccaccgggg
     8761 tttgatgctg attgaatacc tctaccgccc attccaccac acctgttggt ttttcttctg
     8821 cgctacgctg ttttttcagc gtcttgcgct tacaggtgag acgcacctgg atggtatcgc
     8881 ctggctttac gggttcgata aaacgcaagc tttccagccc gtagttagca atgaccggac
     8941 caacaccggc atcgacaaac agacccgcag ccgcagaaag cacaaaatac ccatgcacca
     9001 cccgctcacc gaaaatagat tcggcagcag caatcttatc catatgtgca tagaaatgat
     9061 cgccgctgag gcaagcaaag ttaacaatat cggcctctgt cattgtgcgg cggggagtca
     9121 acaggctgtc gcctggttgt agctcctcaa aatatttgcg gaacggatga atacgatctt
     9181 cttcgacttt cgcaccgcgc acccactgtt tactgatagc ggcaagcatc gtcggactac
     9241 cctgaacagc ggttcgctgc atgtaatgtt tcaccgctcg taaaccacct aattcttcac
     9301 cgcctcctgc gcgaccaggc ccaccatgta ccagttgtgg cagtggggag ccatgcccgg
     9361 tggattcttt tgccgactct tcattgagga tctgaattcg cccatgcgta cgtgccgcgt
     9421 cggcaataaa ctgacgcgca atttgcggat cagccgtcac cagcgttccc gcaaggctac
     9481 cgccgcctgc acaagccagt tgcagagcat gttgctggtt ttgtgctggc attagcgttg
     9541 cgacagggcc aaaggcttct gttgcatgta ccgccggtgt ttcatccggc tgcggacagt
     9601 acaataaggt tggcgggaag aaggcacccg cagcagataa atccgcctga ccaccgaggc
     9661 gaatctcgca tcctgcagcc agcaatatgt tcactttttc ctgcacatcg gcacgctgct
     9721 cagcatttac cagtgcgccc attttcacgc cttcctgagc aggatcaccg accacgactt
     9781 tctgtaatcg cgcaaccaga gcatcactga cagcattaac caatgcctgc ggcacaataa
     9841 tccgccggat tgccgtacat ttttgcccgg cttttgtggt catctcacgc acaacttcac
     9901 gaataaacag cgcaaactcc ggttgatccg gggtgacatc ttcgcccagt acgcagcagt
     9961 tcagggaatc agcttccata gtgaagggga tagatttggc gacgatattt ggctgaactc
    10021 gcagcatctg tccggtcgcc gctgaccccg tgaaagtcac cacatcctgg ctgtccagat
    10081 gatccaacaa gtcgccagca ctaccgcaga tcagactaat tgcgccttcg ggaacaagac
    10141 cactatcgac aattgatttc accatcgcct gagtcagttg ggccgtcgcg gtagctggtt
    10201 tgatgatggc tggcattccg cccagccacg ttggtgccag cttttccagc attccccagc
    10261 aggggaagtt aaaggcgtta atatgcactg ccacgcctga ctttgaggtc agtacatggc
    10321 gcgcggcaaa tccaccttct ttcgataagg ggatcaattc atcttccggc cacagcgtat
    10381 cgtcaggcag ctcccggcta ccgaggctgg cgtaagtaaa taacgtccca atgccacctt
    10441 caatatcaac ccaactgtct gcccgcgttg cgcctgtttg cgcagaaaga gcatagaagc
    10501 gctctttttc actcagcaga tgtttagcga ccgctttaag catcgccgca cgttcgataa
    10561 aggtcatcgc gcggagggcg ggggcacctt tttcaatggc aaactggcgg gcagccgcca
    10621 tatcaagacc ttcactggtc acttcccata acgcctcgcc gctaatagcg tggtgaatca
    10681 aacggctacg gccccggcca gactgccagg taccggataa gaaactggct aactgctgca
    10741 tcgctactct ccagatgttt cacatttctg ttgctaatag ttaaatcgcg aatcataaaa
    10801 agcaaggggt cttttaacga aatgttaact atgcgatctg tatagcaact gcggaaaaca
    10861 ttaatgcact gataaataat gatttataaa aatagggtgc gaaatccgtc acagttcaaa
    10921 catacaaaat ttgtgatttt agttaaccat tgtgtaactt tcataaaaca acgtgattcg
    10981 tgtttttaat taattcacga aaactggaat cgtaaaggtg atgacgtgac ccaagaagaa
    11041 cgctttgagc aacggatagc ccaggaaacg gctatcgagc cgcaggactg gatgcccgat
    11101 gcttaccgaa agacactgat ccgccagatt gggcagcatg cgcactccga gattgttggt
    11161 atgttgcctg aaggtaactg gatcactcgc gcaccaacct tgcggcgtaa agccattctg
    11221 ttagccaaag tgcaggatga agccggtcat ggtctgtatc tctatagcgc cgccgaaaca
    11281 ctgggctgtg ccagagaaga catctaccaa aaaatgctcg acggacggat gaaatattcc
    11341 tccatcttta attatccgac attgagttgg gctgatatcg gcgttatcgg ctggctggtg
    11401 gatggcgcag cgatcgtcaa tcaggtggcg ttatgccgaa cttcttatgg cccgtatgcc
    11461 agagcaatgg tgaaaatctg caaagaagag agttttcacc agcgtcaggg gtttgaagcc
    11521 tgcatggcac tggcgcaggg cagcgaagct caaaagcaga tgttgcaaga cgccattaac
    11581 cgtttctggt ggccagcctt aatgatgttc gggccaaacg atgataactc gccaaacagc
    11641 gccagaagtc tcgcctggaa aatcaaacgt ttcaccaacg acgaactccg ccagcgtttc
    11701 gtggacaaca ccgttccaca ggttgaaatg ctcggtatga ccgttcctga cccggatctg
    11761 cattttgaca ctgaaagcgg tcactaccgc tttggtgaga tcgactggca ggagtttaac
    11821 gaagtaatta acggtcgcgg aatttgtaat caggagaggc tcgacgccaa acgtaaagcc
    11881 tgggaagaag gtacctgggt acgggaagca gcgctggccc atgcacaaaa acaacttgcc
    11941 cgtaaggtcg cataaggaga ttcaaaatga gtaatgttta ctggccgtta tacgaagttt
    12001 tcgtgcgtgg caaacagggc ttatcacacc gccatgttgg cagtttacat gctgccgatg
    12061 agcggatggc actggaaaat gcccgtgatg cttacacccg tcgtagcgaa ggatgttcaa
    12121 tttgggtggt gaaggcgagt gaaattgttg cctcgcaacc ggaagaacgc ggtgaatttt
    12181 ttgatccggc tgaaagcaag gtctatcgcc atccaacgtt ttacaccatc cctgatggca
    12241 ttgagcacat gtgaggtcgg aaatgaatca gttaacggct tacaccttgc gcctgggcga
    12301 taactgtctg gtgctctccc agcggttggg ggaatggtgc ggtcacgcac cggaactgga
    12361 aatcgatctc gcactggcaa acattggcct cgatttatta ggtcaggcac gtaacttctt
    12421 atcgtatgcc gctgaattag cgggagaagg tgatgaagat accctggcct tcacccgaga
    12481 cgagcgccag ttcagcaact tattgttggt tgaacaacca aacggcaatt ttgccgacac
    12541 cattgcacgc cagtatttca tcgatgcatg gcatgtggcg ctctttaccc gtctgatgga
    12601 aagccgtgat ccgcaactgg cggcgatttc tgccaaagca attaaagaag cgcgttatca
    12661 cctgcgtttt agtcgtggct ggctggagcg actgggcaat ggtactgacg tatcagggca
    12721 aaagatgcag caggccatcg acaagttgtg gcgttttact gccgaactgt tcgatgccga
    12781 cgagattgat attgcactga gtgaagaggg tattgcggtt gatccacgca ctttacgcgc
    12841 agcgtgggaa gccgaagttt ttgccgggat caacgaagcc acattgaacg taccgcaaga
    12901 gcaggcgtat cgcactggcg gtaaaaaagg actgcatacc gaacaccttg gacccatgct
    12961 ggcagaaatg cagtatctcc agcgtgtctt gcccggtcag caatggtaac agaggagatg
    13021 ggtatgcaac gtctggccac cattgcaccg ccacaggttc atgagatatg ggcgctactc
    13081 agccagatcc cggacccgga gatcccggtg ctgaccatta ccgatttagg catggtgcgt
    13141 aatgtgacac agatgggaga aggatgggtg atcggcttta cgccgactta ttccggttgt
    13201 ccggcaacgg aacatttgat tggcgcgata cgtgaggcaa tgacaaccca cgggtttacc
    13261 cccgttcagg ttgtgctgca actcgacccg gcatggacca ccgactggat gacccccgat
    13321 gcccgtgagc gtctgcgaca gtatggcatt agcccgcccg ccggacacag ttgccatgcc
    13381 catttgccgc cagaagtacg ttgcccgcgc tgcgccagcg tccataccac acttatcagt
    13441 gaatttggtt ccacggcctg caaagcattg tatcgctgcg atagttgccg cgaacctttc
    13501 gattatttca aatgtatttg aggatgccat gacaacgttt cattccttaa cggtggcaaa
    13561 agtggagccg gaaacccgtg atgcggtgac cattaccttt gcggtgcccc agcctttgca
    13621 ggaggcgtat cgctttcgcc ccggtcaaca tttgacctta aaagccagct ttgatggtga
    13681 agaattacgc cgttgttact ccatttgccg cagctatctg cctggcgaaa ttagtgtggc
    13741 ggtgaaagcc attgaaggcg gacgtttctc ccgctatgcc cgcgaacaca tccgccaggg
    13801 tatgacgctg gaggtcatgg tgccgcaggg gcatttcggc tatcagccac aggccgaacg
    13861 ccagggccgc tatctggcta ttgcagcagg atcaggtatt acgccaatgc tggcgattat
    13921 cgccaccact ttacaaaccg agcctgaaag tcagttcacc ctgatctacg gtaaccgtac
    13981 cagccagagc atgatgtttc gccaggcact ggcagacctg aaagacaaat atcctcagcg
    14041 tttacagttg ttgtgcattt tcagtcagga aaccctcgac agcgatctgc ttcacgggcg
    14101 tattgacggt gaaaaattac agtcacttgg ggcctcgctc attaattttc gtctttatga
    14161 tgaggcattt atttgtggtc cggcggcgat gatggatgag acggaagccg ccttaaaagc
    14221 actgggaatg ccagataaaa ccattcatct ggagcggttt aatacgcctg gcacgcgcgt
    14281 caaacgtagc gttaacgtgc aaagtgacgg acaaaaagtg actgtacgtc aggatgggcg
    14341 ggatcgggaa atcgtgctta atgccgacga tgaaagcatt ctcgatgcgg cattgcgcca
    14401 gggggctgat ctgccctatg cctgcaaagg cggcgtctgt gcgacctgca aatgcaaagt
    14461 gctgcgtggc aaagtggcga tggaaaccaa ttacagtctg gaaccggatg aactggccgc
    14521 aggctatgtg ttgagttgcc aggcactgcc gctgaccagc gatgtggtgg ttgactttga
    14581 cgcgaagggg atggcatgag cgaactgatc gtcagccgtc agcaacaagt attgttgctg
    14641 acccttaacc gtcccgccgc acgtaatgcg ctaaataatg cccttctgac gcaactggta
    14701 aatgaactgg aagctgcggc taccgatagc agcatttcgg tctgtgtgat taccggtaat
    14761 gcacgctttt ttgccgctgg ggccgatctc aacgaaatgg cagaaaaaga tctcgcggcc
    14821 accttaaacg atacacgccc gcagctatgg gcgcgattgc aggccttcaa caaacctctc
    14881 atcgcagccg tcaacggtta cgcgcttggt gcgggttgcg aactggcatt gttgtgcgat
    14941 gtggtggttg ccggagagaa cgcgcgtttt ggtttgccgg aaatcactct cggcatcatg
    15001 ccaggcgcag gaggaacgca acgtttaatc cgtagtgtcg gtaaatcgtt agccagcaaa
    15061 atggtgctga gcggagaaag tatcaccgct cagcaagcac agcaggccgg gctggttagc
    15121 gacgtcttcc ccagcgattt aaccctcgaa tacgccttac agctggcatc gaaaatggca
    15181 cgtcactcgc cgctggcctt acaagcggca aagcaagcgc tgcgccagtc gcaggaagtg
    15241 gctttgcaag ccggacttgc ccaggagcga cagttattca ccttgctggc ggcaacagaa
    15301 gatcgtcatg aaggcatctc cgctttcttg caaaaacgca cgcccgactt taaaggacgc
    15361 taatgatgga attcatcctc agtcatgtag aaaagggcgt gatgacacta acgctcaacc
    15421 gcccggaacg cctgaacagt tttaatgatg agatgcacgc acaactggca gagtgcctga
    15481 aacaggtcga gcgcgacgac actatccgtt gcctgttact tacaggtgcc gggcgcggat
    15541 tttgtgctgg tcaggatctt aacgatcgta acgttgatcc cacaggcccc gcaccggatt
    15601 tagggatgtc agttgaacgt ttctataacc cactggtacg tcgcctggca aaactgccaa
    15661 aaccggtgat ctgtgcagtc aatggcgtgg cggcaggggc tggcgcaaca ctggcactgg
    15721 gatgcgacat cgttattgct gcccgttcag caaaattcgt catggcgttt agtaagttag
    15781 gtttagtacc cgattgcggt ggaacctggt tactgccacg cgttgctggg cgagcgcgcg
    15841 ccatggggct ggcactgctg gggaatcaac tgagtgctga acaggcgcac gaatggggga
    15901 tgatctggca ggttgttgat gatgaaacgc tggcagatac cgcgcaacag ctggcacggc
    15961 atctggcgac acaaccgaca tttggtcttg gacttatcaa gcaagcgata aatagcgctg
    16021 aaaccaatac gctcgatacg caactggatc tggaacgcga ctatcagcga cttgccggac
    16081 ggagcgcaga ttatcgtgaa ggtgtcagtg cgttcctggc taaacgctca ccgcagttca
    16141 cggggaaata gcatgatgat aaatgtgcaa actgtggcag tgattgggag cggcaccatg
    16201 ggggctggca ttgctgaagt tgccgccagt catggacatc aggttttact gtatgacatt
    16261 tctgctgaag cgctgacccg cgcaatcgac gggatacacg cgcggctaaa ttcacgcgtg
    16321 acgcggggaa aactgactgc tgaaacctgt gaacgcacat tgaaacgcct gatcccggtg
    16381 accgatattc acgcgctggc agctgcggac ctggtcattg aagcggcgtc tgaacgtctg
    16441 gaagtcaaaa aagcgctctt tgcacagctg gcggaagttt gcccgccaca aacgctattg
    16501 accactaaca cttcgtcaat ctctataacc gcgattgctg cggaggtaaa aaatcctgaa
    16561 cgtgttgcgg ggctgcattt ttttaacccg gcaccggtga tgaagttggt ggaggtggtc
    16621 agtgggctgg caacggcagc ggaagttgtt gagcagttgt gtgaactaac gttgagttgg
    16681 ggtaagcagc ctgtgcgctg tcattcgacc cctggattta tcgttaaccg tgtggcgcgt
    16741 ccttattatt ccgaggcctg gcgggcactg gaagagcagg ttgctgcacc agaagtgatt
    16801 gacgctgcac ttcgcgatgg cgctggtttc ccgatggggc cgctggaatt aaccgatctg
    16861 attggtcagg acgtcaattt tgctgtcacc tgttcggtgt ttaacgcttt ctggcaggag
    16921 cgtcgttttt taccttcgct ggtgcaacag gaactggtga ttggtggacg gttgggcaag
    16981 aaaagtgggc tgggcgtgta cgactggcgc gcggaacgtg aggcagttgt tggcctggaa
    17041 gcggtaagcg acagttttag cccaatgaaa gtagaaaaga aaagtgacgg tgtcacggaa
    17101 attgacgatg ttttattgat tgagacacaa ggcgagacgg cacaggcgct ggcaatacga
    17161 ctggcacgcc cggtggtagt ggtcgacaaa atggcgggca aggtggtgac cattgctgcg
    17221 gcagcggtga acccggactc agcgacccgc aaggccattt attacctgca acagcagggc
    17281 aaaacagtgc tgcaaattgc agattaccca ggaatgctga tttggcgaac ggtagcaatg
    17341 atcatcaatg aagcccttga tgcgcttcaa aaaggcgtgg cctctgaaca ggatatcgat
    17401 accgccatgc gtcttggggt gaattatcca tatggcccac ttgcctgggg agcgcaactt
    17461 ggctggcagc gaatattaag gctccttgaa aatctacagc atcactatgg cgaagaacgc
    17521 tatcgcccat gttcattgct gcgccaacgg gcgcttctgg agagcggtta tgagtcataa
    17581 ggcctggcaa aatgcccatg caatgtatga gaacgatgcc tgcgccaaag cgcttggcat
    17641 cgacattatc tcaatggatg aaggctttgc tgtagtgacc atgaccgtca ctgcacaaat
    17701 gcttaacggt catcaaagtt gccacggcgg gcagctattt tcactggctg atactgcctt
    17761 tgcctacgcc tgcaatagcc aggggctggc agccgtcgct tctgcctgca cgattgattt
    17821 tttacgtcca ggctttgccg gagacacctt aactgcgact gcgcaggtac gtcatcaggg
    17881 caagcaaacc ggtgtttacg acatcgaaat tgttaaccaa caacaaaaaa cggttgcgct
    17941 gtttcgcggt aaatctcacc gcatcggcgg caccattaca ggagaagcct gatgcgtgaa
    18001 gcctttatct gtgacggaat tcgtacgcca attggccgct atggtggggc attatcaggt
    18061 gttcgggctg acgatctggc tgctatccct ttgcgggagc tgctggtgcg aaacccgcgt
    18121 ctcgatgcgg agtgtatcga tgatgtgatc ctcggctgtg ctaatcaggc gggagaagac
    18181 aaccgtaacg tagcccggat ggcgacgtta ctggcggggc tgccgcagag tgtttccggc
    18241 acaaccatta accgcttgtg tggttccggg ctggacgcac tggggttcgc cgcacgggcg
    18301 attaaagcgg gcgatggcga tttgctgatc gctggtggcg tggagtcaat gtctcgggca
    18361 ccgtttgtta tgggcaaggc aaccagtgca ttttctcgtc aggctgagat gtttgatacc
    18421 actattggct ggcgatttgt gaacccgctc atggctcaac aatttggaac tgacagcatg
    18481 ccggaaacgg cagagaatgt agctgaactg ttaaaaatat cgcgagaaga tcaagatagt
    18541 tttgcgctac gcagtcagca acgtacggca aaagcgcaat cctcaggcat tctggctgag
    18601 gagattgttc cggttgtgtt gaaaaacaag aaaggcgttg taacagaaat acaacatgat
    18661 gagcatctgc gcccggaaac gacgctggaa cagttacgtg ggttaaaagc accatttcgt
    18721 gccaatgggg tgattaccgc aggcaatgcc tccggggtga atgacggagc cgctgcgttg
    18781 attattgcca gtgaacagat ggcagcagcg caaggactga caccgcgggc gcgaatcgta
    18841 gccatggcaa ccgccggggt ggaaccgcgc ctgatggggc ttggtccggt gcctgcaact
    18901 cgccgggtgc tggaacgcgc ggggctgagt attcacgata tggacgtgat tgaactgaac
    18961 gaagcgttcg cggcccaggc gttgggtgta ctacgcgaat tggggctgcc tgatgatgcc
    19021 ccacatgtta accccaacgg aggcgctatc gccttaggcc atccgttggg aatgagtggt
    19081 gcccgcctgg cactggctgc cagccatgag ctgcatcggc gtaacggtcg ttacgcattg
    19141 tgcaccatgt gcatcggtgt cggtcagggc atcgccatga ttctggagcg tgtttgagca
    19201 tatcaacctg cgagtaccct acaatgataa ccaatacaaa gcttgacccg attgaaaccg
    19261 cgtctgttga tgagttacag gcgttgcaaa cacaacgtct gaaatggacg ctcaaacacg
    19321 cgtatgaaaa tgtcccgatg tatcggcgca aatttgacgc agcaggcgta catcctgatg
    19381 atttcaggga actttcagat ctgcgtaaat ttccctgtac caccaaacag gatctgcgcg
    19441 acaactatcc cttcgacacc tttgccgtgc cgatggaaca agtggtgcgc attcatgctt
    19501 cttcgggaac cacaggtaaa ccgacagttg tcggctatac gcaaaacgat attgataact
    19561 gggccaatat tgtagcgcgt tccttgcgtg cggcaggtgg cacgcccaaa gacaaaattc
    19621 atgttgccta tggttacggc ctgtttactg gtgggctggg cgcgcactat ggtgccgaac
    19681 gtctgggcgc tacggtgatc ccgatgtctg gtgggcagac ggaaaaacaa gcgcaactga
    19741 tccgcgattt tcaaccagat atgatcatgg ttacgccatc ttattgcctt aacctgattg
    19801 aagagctgga gcggcagttg ggcggtgatg ccagcggttg ctcgctgcgg gttggagtgt
    19861 ttggtgctga gccgtggaca caggccatgc gtaaagagat tgaacggcgt ctggggatca
    19921 ccgcactgga tatttatggc ctgtcagaag tgatggggcc gggggtggcg atggagtgtc
    19981 tggaaactac cgacggccca accatttggg aagatcattt ctatcctgaa attgttaatc
    20041 ctcatgacgg cacaccgctt gccgatggtg aacatggcga actgttattc accacgctga
    20101 ccaaagaagc attgccggtc attcgttacc gcacgcgtga tttaacccga ctgttaccag
    20161 gaacggcgcg gactatgcgc cgcatggatc gcatcagcgg acgcagcgat gacatgttga
    20221 tcattcgcgg tgtaaatgtc ttcccgtcac aactggaaga agagattgtc aaattcgaac
    20281 atttatcgcc ccattaccaa ctggaggtga accgccgggg gcatcttgat tcactttctg
    20341 tgaaagtgga gttgaaagaa agcagcctga cattgacgca tgagcagcgt tgccaggtgt
    20401 gccaccagct gcgccatcgg attaagtcga tggtggggat ctctaccgat gtgatgatcg
    20461 ttaactgtgg cagtatcccg cgttcagaag gcaaggcgtg tcgggtgttt gatctgcgca
    20521 atattgttgg tgcctgacgt gtcgtctggc cctggtgagg taaagcgcca gggccagaag
    20581 tcgatacgac ctgtgctatg attcataaat cacaacaata acaacagact gaatcgaatg
    20641 agtaaacttg ttacttttat ccaacatgct gtaaacgctg ttccggtcag tggcacatct
    20701 ttgatctcct ctctgtatgg tgattcgctt tcccatcgtg gtggtgaaat ctggctgggt
    20761 agtctggctg ctttgctgga agggctggga tttggtgagc gtttcgtgcg caccgctttg
    20821 tttcgtctta ataaagaagg ctggctggat gtttcccgca tcgggcgacg cagtttctat
    20881 agcctcagtg ataaaggctt gcgcctgacg cgacgggcag aaagtaaaat ttatcgcgca
    20941 gagcaacctg catgggatgg taaatggctc ctgttgctct cggaaggttt agataaatca
    21001 acgctggctg atgtcaaaaa gcagttgatc tggcaaggtt ttggcgcact ggcacccagc
    21061 ctgatggcat cgccgtcgca aaaactggcg gatgtacaga cacttttgca tgaagcgggg
    21121 gtggcggata acgtgatttg ttttgaagcg caaataccac tggcgctttc tcgtgcagca
    21181 ctgcgtgcca gagtggaaga gtgctggcat ttaactgaac aaaatgccat gtacgaaacc
    21241 tttattcagt cattccgccc gctggtgccg ctgttaaaag aggcagcaga cgagttaacc
    21301 ccggagcgtg catttcatat tcagctttta ctgatccatt tttatcgccg tgtcgtcctt
    21361 aaagacccat tgttgccaga ggagttgctt ccggcgcact gggcagggca tacggcgcgt
    21421 cagctgtgta tcaacattta tcagcgcgta gcgcctgctg ctttagcgtt cgttagtgaa
    21481 aaaggtgaaa cctcggtcgg tgaactgcct gcgccgggaa gcctgtattt tcaacgtttt
    21541 ggcggtttga atattgaaca ggaggcgata tgccaattta ccagatagat ggtctgactc
    21601 cggttgtgcc agaagagagt tttgtccatc cgacagcggt attgatcggc gatgttattc
    21661 taggcaaggg cgtttacgtt gggccaaatg ccagcctgcg tggcgatttt ggtcgtatcg
    21721 tggtgaaaga tggcgcgaac attcaggata attgcgttat gcacggtttt cccgagcagg
    21781 atactgtcgt ggaagaagat ggacatattg gtcatagcgc tatccttcat ggttgcatta
    21841 tccgccgcaa tgcattagtg ggaatgaacg cggtagtgat ggacggcgcg gtgattggcg
    21901 agaacagcat tgttggcgca tccgcatttg tgaaagccaa agcagaaatg cctgccaatt
    21961 acctgattgt cggcagcccg gcgaaagcga ttcgtgaact cagtgagcag gagctggcat
    22021 ggaaaaagca gggtacgcat gagtatcagg tgctggtgac tcgttgtaag cagacgttgc
    22081 atcaagttga gccattgcgg gaagttgaac cgaacaggaa acgtctggta tttgatgaga
    22141 atctgcgacc gaaacagtaa cgtccagata ctgaacattc taatttttag catgtgatac
    22201 ctaaaccgca acgctgatac aggttgcggt ttttttattg ccggatgcgg cgttttacgc
    22261 gttttgtttt gtgtttttca ggacaataga gcaactcatc cagtaatctt gtttacgccc
    22321 tcgcattatt tatctctttt cgtttctata ctgatttttc ttaatccgtt ttattacagg
    22381 gcagggtgcg atgagcagca atacatttac tctcggtaca aaatccgtta accgtcttgg
    22441 ttatggcgcg atgcaactgg caggtcctgg ggtttttggt cccccaagag atcgccacgt
    22501 cgctataact gtgctgcgcg aggcgctggc attgggcgtc aatcacattg ataccagcga
    22561 cttttatggt ccgcacgtca ccaatcagat tatccgcgaa gcgctttatc cttactctga
    22621 cgacctgaca attgtcacta aaattggtgc gcggcgtgga gaggacgctt cctggttgcc
    22681 cgccttttct ccggcagagt tgcaaaaagc ggtgcacgat aatctacgta atctcggcct
    22741 ggacgtactg gatgtggtta acttgcgcat tatgatgggg gatggtcatg gcccagcgga
    22801 aggatcgatt gaagccagcc tgaccgtgct ggcagagatg caacaacaag gcctggtaaa
    22861 acatattggc ctaagcaacg tcacaccgac gcaggttgca gaggcgcgca agattgccga
    22921 aatcgtctgt gtgcagaacg aatacaacat tgctcatcgt gctgatgacg caatgattga
    22981 tgctttggct cgtgacggca ttgcctacgt gccgttcttc ccgctcgggg gctttacacc
    23041 gctgcaatcc tctacgctgt cggatgttgc tgcgagcctg ggtgcaacac ccatgcaggt
    23101 ggcgctggcg tggctgttac aacgttcacc gaatattttg ctgatcccag ggacatcttc
    23161 tgttgcgcat ttacgggaga atatggctgc tgaaaaattg catctttctg aggaagtgtt
    23221 gtctacgttg gatggtattt cgcgagaata acgaatatac aaaagggaaa gatgcatttc
    23281 ccttttgttt cttttttaat ggcatggagt gcatatgttt aagcccagga tttgcagctg
    23341 gataggttta cttcccctgt ttatgctgag tcttccagta caggcggaac ttcgatgtgt
    23401 tgcaaatgca gttaatattg aaccattttt ttctgcagct accgctgaag ataaacaaca
    23461 agttgaacaa gctatcaaca gtagcgtgaa tcttgtcccc ttcggtttat ctgcatcgga
    23521 ctggaaagtg catcgtggcg atttagtggt agaaggtaat atagagagta atcaaaaatt
    23581 gattgttctt ggcaatttga cagtcaaagg taatatttcc actttctctc tttctaatcc
    23641 atgggttatt ctcggtaacg tgacggcaac gaatatagtt acggattcac cactgttaat
    23701 taccggttcg ataaatgcaa gtggactggt atttatcgac tcatattacg ataatccgtc
    23761 tacgattaag gggagtatta atgcgcgtgg gatatttatc aatgacataa ttgcacctgt
    23821 tgttgcgtct tcgacaaata gtgaattcat ggtccgtgcg agtgacaaaa atgacactga
    23881 aaatgtcaaa aaagcgctga tgataataaa tcctgatgca tattattggg gtctaataaa
    23941 tgatgaagat gctctgaaag aaatttttaa gcgaagcaat attcgcatgg cagggaatgt
    24001 ctgtaatcag atgaaaaaag aagcgctgtt tcgccctaag ccttctcctg agttagtgca
    24061 ggaattgcaa atgctggatg aaggcaacgt tgctgcattt gaaggacgag atattgcgac
    24121 atttgatctt gccataatgc gaactctccc aaggcttaaa ggaatttcag ctaacctacg
    24181 caaacaactt attaatagca atgatgaaca aacgattgaa agtatggcga gatatatgcc
    24241 tgacaatgag attctggagt tgaccgatca gcaattgggt taccagcctg ttgttctggg
    24301 gttgctcgac cgtgaaccgc tctctgttga aataatgacg cgaatgagtc gtctacctga
    24361 tggtgtcggt ccgttgaatc ttgcgctacg tgaaaatctc cctctggata tcgtcatgac
    24421 tctggcgaaa agagattggg atatgatcat tcaggagctt tataaagatg cgtggttatt
    24481 gcctgaatcc attattgatg gctatatccg cagtgatgat tcctctattc gtcaggtcgg
    24541 tgctggagga caactcacct acaatcaggc aatgcagttg gcgaacgatt catcaaacaa
    24601 tgttgtcaca agcttagcgt tcaagctggc agagatgaaa caccatggtc aattattgcg
    24661 gatgacgcca caagagagtg acaaagttgc agcctattta taccaaaaat tcgagaatga
    24721 cgatgatcta atacgtgtgt tatttttagc attgccagat aacttacagt ttaattttgt
    24781 taaaaggatg gagaaaaaat cccctgccta cttttgctgt cgggatatgc aggtaattca
    24841 ctctgacgct gctctacaac ggttattgac acgtttcaac gatcctgagg ggtggagtaa
    24901 tctggcgaaa aatcagtatc tgagcacgtc gatgaaacaa aaaatttggc aacgtgcttt
    24961 aacgcatcgg aaaaataatc cgaaagcaga ttcagctgcg tatgaaacca gcgctgatat
    25021 gattttgtct gagcttatca gccacggtga agttgatgat cagatgctat taaatgccac
    25081 agccctgata cgtttggaag actgggactt tttagagagt gcattagtta gttgggataa
    25141 tttacccgtt gttgtcctca aggaattaca gcaaaatacg ccacgcaatg atatttgggc
    25201 gaagtttttt ctgaggcagg aaaacagctc ccgtgcgcag gttgatgaag cattacgcgt
    25261 ctattatgca ctcgaccccg atgcgttagc acaactggat gtactggcaa aacaaccgga
    25321 tcgtatatgg tggagtacac tggcgaaaag caatctcaca tttttcaagt tcggcgcact
    25381 taacaaccgc cacacaccgc ctgcagtact ggcggcagaa attgatcccg agtggtggat
    25441 tgtggcgatg aataatcccc gttttccagt tgatgtatta aaggcgaggc tgaaacgtga
    25501 tcctttgctg gcgttagagc ttgttaaccc tgaactggat ttagtccgcc agctggcgct
    25561 taacggtaag acgcgcgcaa tacgggaaca agcgatgaga aaacttgatg agttgtattg
    25621 atccacaacg taatatttct taaccctttg attaattggg gataattatt cctgacagtg
    25681 agtccccaat accttgatat attctgaatt tttaatgaag cggcgtgttg cgatatctcc
    25741 gtcaggggaa ttgatgcacc atagcgcaaa ccgaattatc aaggattgat aatgacgctc
    25801 taccagataa aaccgctctt tcagtcgctg ttaaggccga cgatgttttg gctttacaag
    25861 caccacgtta cagcgaatca catcactctt gctgcactgg cgctttctct tcttaccgga
    25921 ttgctgttga tgttggtggc acaacccatc ctctttatgc tattgcccat cgtgcttttt
    25981 atccgtatgg cgctcaatgc gctggatggc atgttggcgc gtgagtgcaa tcagcaaaca
    26041 cggcttgggg caattttgaa tgagactggt gatgttattt ccgatattgc gctctattta
    26101 ccatttttat ttttaccgga aagtaacgca tcactcgtga tactcatgtt attttgcacc
    26161 atattgaccg agttttgcgg tttactcgcg cagacgatta acggtgttcg cagttatgcc
    26221 ggtccatttg gcaaaagcga tcgcgcgtta atatttggtc tgtggggtct ggccgttgcc
    26281 atttatccac agtggatgca gtggaataat cttttatgga gcattgcttc aattctgctt
    26341 ctctggactg cgattaatcg ttgtcggagt gtgcttctta tgcgcgctga aagataatgc
    26401 tggaaaaatc tctggcaaca cttttcgcgt tgttaatttt agcaacgctg ataaaccatt
    26461 ttctgatatg gcggttaccg gagagaaaag aggatgaagt tacattacgt attcgtacct
    26521 ggtggggcat cgtcatttgt ttttcactgg tgatttcagg tccacgctgg atgacgttga
    26581 cgttttttgc gctgataagt tttctggcat tgaaagaata ttgtacgctt atatctgtac
    26641 attttccgcg gtggttatat tgggttattc ctcttaacta tttgtttatc ggttttaatt
    26701 gctttgagct gtatctatta tttatacctt tggctgggtt tctaatatta gccaccgggc
    26761 aagtttttat gggggacccc tccggttttc tgcataccgt gagcgccatt ttttggggct
    26821 ggataatgac cgttttcgcc ttgagtcatg ccgcctggtt attaatgttg ccaaccacaa
    26881 atatccaggg cggggcgtta ctggtgttat ttcttttggc attaaccgag tcaaacgata
    26941 ttgcacagta tttatggggg aaatcctgcg gcagaagaaa agtggtcccc aaagtcagcc
    27001 cgggaaaaac gctggaaggt ctggtggggg gcgtaatcac caccatgatt gcgtcactga
    27061 ttattggacc gttgctgaca ccgctaaata cattacaggc attattagcg ggtttgttaa
    27121 ttggtattag tggtttttgc ggcgatgtcg tgatgtcagc catcaaacga gatattggtg
    27181 ttaaagatag tgggaaacta ttaccaggac atggcggact ccttgacagg attgactcat
    27241 taattttcac cgctccggta tttttttatt ttattcgcta ctgctgttac tgaaggaaaa
    27301 tagagaaaat ggaaaattca cgcatccatg gggaacattt ttttaccagc agtgataata
    27361 cagcgttgtt ttatcggcac tggcccgctt tacagcccgg ggctaaaaag gtcatcgtct
    27421 tatttcatcg cggtcatgaa cattctggtc gtctacaaca tctcgttgat gaactggcga
    27481 tgccagatac tgttttttat gcctgggatg cccgagggca tggacaaact tcggggccgc
    27541 gtggttatag cccatctctt gcgcgttcag tgcaggatgt cgatgaattt gttcgttttg
    27601 ctgccagcga tagccaggtc ggactggatg aggtggtagt gatcgcgcaa agcgtcggcg
    27661 cagtgctggt tgccacatgg gttcatgatt atgcgcctgc aattcgcggg ctggtgctgg
    27721 cttctccggc ctttaaggtt aaattgtatg taccgctggc acgtcctgcg ctggcgttat
    27781 ggcatcgtct gcgtggtctg ttttttatta attcctatgt gaaaggacgc tatttgaccc
    27841 acgatcggca acgggtggcg agtttcaata atgatccgct gatcacacgg gcgattgccg
    27901 ttaatatctt gctcgatctc tacaaaacgt ctgaacgtat tattagagat gcggcggcga
    27961 ttacgctccc cacgcaactt ctgatatcag gcgatgacta tgtggtgcat cgccaaccgc
    28021 agattgattt ttatcagaga ttacgtagcc ctctgaaaga gctgcatctg ctgccaggct
    28081 tttatcacga cacgttgggt gaagagaaca gggcgcaggc atttgaaaaa atgcaaagct
    28141 ttattagtcg tttatatgct aacaagtcac aaaaatttga ttatcagcat gaagaccgca
    28201 ctggaccatc agcggatcgc tggcggctcc tttcaggtgg acccgtgcca ttatcgccgg
    28261 ttgatttggc gtatcgcttt atgcgaaagg cgatgaaatt gttcggggcg cactctgcgg
    28321 gcctgcatct cggaatgagc accggctttg attcaggcag ttccctggat tatgtctatc
    28381 aaaatcaacc gcaaggcagt aacgcattcg ggcgcttaat tgacaaaatc tacctgaaca
    28441 gtgttggctg gcgcggtatt cgccagcgta aaacccattt acaaatactg attaaacaag
    28501 ccgttgccga tctccacgcc aaaggtttag ccgtccgcgt ggttgacatt gccgcagggc
    28561 atgggcgcta tgtactggat gcgctggcaa acgagcctgc cgtaagcgat attttgttac
    28621 gtgattacag tgagttaaat gttgcacagg ggcaagcgat gattgcccaa cggggaatgt
    28681 ctgggcgggt gcgttttgaa cagggcgatg cgtttaaccc ggaggaactc agcgcgttaa
    28741 ctccgcggcc tacgctggcg attgtctctg gcctgtatga gctttttccc gaaaatgagc
    28801 aggtaaaaaa ctcactcgca ggtcttgcca atgccatcga accgggcggc attctcatct
    28861 acaccgggca gccgtggcac cctcaactgg agatgattgc cggggtgtta accagtcata
    28921 aagatggtaa accgtgggta atgcgcgtgc gttcgcaagg ggagatggat tcactcgtgc
    28981 gtgatgccgg atttgataaa tgcacacaac ggattgatga gtggggtatt tttacggttt
    29041 cgatggcggt gcgtcgtgat aactgaacgt cggaacgtat tgctacaagg cgctggctgg
    29101 ttattgttgc tggccccgtt tttcttcttc acctatggat ctcttaatca gttcaccgcg
    29161 gttcaggacc ttaacagcca tgatatcccc agtcaggtat tcggttggga aacggcgatc
    29221 ccttttcttc cctggactat tgttccttac tggagtctgg atcttttata tggattttcg
    29281 ctgttcgttt gtagcacgac attcgaacag cgccgacttg tccaccggct tattctggca
    29341 acggtaatgg cctgctgcgg ttttttgctc tatccgctga agtttagttt tatccgtcct
    29401 gaagtgagtg gggtgacggg atggctattt tcgcaacttg aactgtttga tctgccttat
    29461 aaccagtctc cttcgctgca tattattctc tgctggctac tttggcgtca ctttcgtcag
    29521 catctggctg agaggtggcg taaagtctgt ggcggatggt ttttactcat cgctatttct
    29581 actctgacga cctggcagca tcattttatt gatgtcatca cagggctggc ggtaggtatg
    29641 ttgattgact ggatggtgcc cgtcgatcgt cgttggaatt atcagaaacc tgatcaacgt
    29701 cgaatcaaaa tagcactgcc atatgtcgta ggcgcgggct cgtgcattgt gttgatggag
    29761 ctaatggtga tgattcagtt atggtggtca gtctggttat gttggccagt attatcgcta
    29821 ctcattattg gccgggggta cggtgggctt ggcgcgataa caacagggaa agatagtcag
    29881 gggaaactcc cgcccgccgt ttactggctg acattgccct ggcgcattgg gatgtggcta
    29941 tctatgcgtt ggttttgtcg tcgcctggag ccggtgagca aaattactgc tggtgtttat
    30001 ttaggggcgt ttccacgaca tattccggca cagaatgcgg ttctggacgt cacctttgaa
    30061 ttccctcgcg gacgagccac aaaagatcga ctctattttt gtgtaccgat gctggatctg
    30121 gtggttccgg aagaggggga gctccgacag gccgtggcga tgctggaaac attacgcgaa
    30181 gagcaaggca gcgttctggt ccattgtgca ttgggattat cgcgcagtgc gctggtggtg
    30241 gcggcatggt tgttatgtta cggacactgt aaaaccgtta atgaagcgat tagctatatt
    30301 cgagcaagac gcccgcagat tgtgctgaca gacgagcaca aagcgatgct gaaattatgg
    30361 gaaaacagat aagtggattg ggatgtggac tgaactacag tccacatcaa gatcgtgtcc
    30421 ggttatgcag caacaatgct gtcgatggcc gctttcgcgt cagactgcgc tttcgctgcc
    30481 atttccggac cgtatgcgat cccttcggcg aagacaaatt tcacatcggt aatgccgata
    30541 aagccgagga acgtggacag atacggcgtc accaggtccg ttggtccatc tttgtggatc
    30601 ccaccgcggc tggtaataac gatggctttt ttacccgtta tcagaccttc cggaccgttc
    30661 tcggtatagc gaaaagtaac gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga
    30721 gttgagatgt tgaagttata catcggtgcc gcaataacga taacgtcgtg ggctttcagc
    30781 tcggcaatca gctcatcgga aagcgccaga gcttcctgct gacgcggagt cagcggcgca
    30841 tcgctcggac gcagagcgcc aaccagttcg ccatccagta ccggaatcgg atttgcagcc
    30901 aggtcgcgaa cggtgatttc atcagcggag tgcttttcgc gccattgttc aacaaaataa
    30961 tcggacaatt gattagactg agagtaccct gccaggatgc tggatttaag aactaatacc
    31021 ttgctcatgg tgtttcctta tagatgtttg aatgggcgat gccccgttgc ttgctgacac
    31081 tttattcaca atcctgccac agagatagcg caataaatcg aagcctatgt tcgaatttat
    31141 tgaacaacgc atagaaagct gcgatgtggt actctatatc tatcatttaa aagaaaatta
    31201 atcaggcaga ctactgccca ctaacgttat gacagaacaa caaaaattga cctttacggc
    31261 cttgcagcag cggctggatt cgctgatgct gcgcgacaga ctgcgttttt ctcgccgtct
    31321 gcacggtgtg aagaaggtta aaaatcctga tgcacaacag gccattttcc aggagatggc
    31381 gaaagagatt gaccaggcgg cagggaaagt cctgctgcgt gaagcggcac gaccggaaat
    31441 tacttatcct gacaatttac cggttagtca gaaaaagcag gacattctcg aagcaattcg
    31501 tgatcaccag gtagtgatcg tcgccgggga aacgggttct ggtaaaacga ctcagctgcc
    31561 gaaaatctgt atggagctgg ggcgcgggat taaaggactg atcggccata cccagccgcg
    31621 tcgcctggcg gcaagaacgg tggcgaaccg tattgcagaa gagctgaaaa cggagccggg
    31681 cggttgcatc ggttacaaag tgcgtttcag cgatcacgta agtgataaca cgatggtcaa
    31741 gctgatgacc gacggtatcc tgctggcgga gatccagcaa gaccgcctgc tgatgcagta
    31801 cgacaccatc attattgacg aagcgcacga acgcagcctg aatatcgatt ttctgctcgg
    31861 ctatttgaaa gagttgctgc cgcggcgtcc tgacctaaaa atcattatca cttccgcgac
    31921 tatcgacccg gaacgctttt cgcgccactt taataatgcg ccgattattg aagtctccgg
    31981 taggacctat ccggtggaag tgcgctatcg cccgattgtt gaagaagccg atgacaccga
    32041 gcgcgatcag ttgcaggcga tttttgacgc cgtagacgaa ctaagccagg aaagccccgg
    32101 cgacattctg atctttatga gcggcgagcg ggaaattcgc gataccgccg atgcgctgaa
    32161 caagctgaac ttacgccata ccgaaatctt gccgctttat gcgcggcttt cgaacagcga
    32221 acagaaccgc gtgttccagt cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc
    32281 ggaaacctcg ctgaccgtac cggggattaa atacgtcatc gaccccggta cagcgcgtat
    32341 cagccgctac agctatcgca ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc
    32401 gtctgccaat cagcgtaaag gccgctgtgg tcgtgtgtcc gaagggatct gtattcgtct
    32461 ctattccgaa gacgatttcc tctcgcgccc ggagtttacc gatccggaga ttctgcgtac
    32521 caacctggcc tcggttattt tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt
    32581 cccgtttgtc gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga
    32641 actgggcgcg atcaccacgg atgaacaggc cagcgcctat aaactgacgg cgctcggtcg
    32701 ccagctctct cagttgcctg tcgacccacg tctggcgcgt atggtgctgg aagcgcaaaa
    32761 acatggctgc gtgcgtgagg cgatgattat cacgtccgcg ctctccattc aggatccgcg
    32821 cgagcgtccg atggataaac agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa
    32881 agaatccgat ttcctcgcgt ttgtaaatct gtggaattat cttggcgagc agcaaaaggc
    32941 gctttcttct aacgccttcc gtcgactgtg tcgtaccgat tatctcaact atctgcgcgt
    33001 gcgcgaatgg caggatatct acacccagtt gcgccaggtg gtgaaagaac ttggcattcc
    33061 ggtcaacagc gaaccggcgg aatatcgcga aattcacatt gcgttgctga cgggtttgct
    33121 ttcccatatc ggcatgaaag atgccgataa acaagaatat accggcgcac gtaacgcgcg
    33181 tttctccatc ttccccggtt ctggtttatt caaaaaaccg ccgaaatggg taatggtggc
    33241 ggaactggta gaaaccagcc gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg
    33301 ggtggagcct gtcgctcagc atttgattaa acgcacctac agcgaaccgc actgggaacg
    33361 ggcgcagggc gcggtgatgg caacggaaaa agtcaccgtt tacgggttgc cgattgttgc
    33421 cgcgcgtaag gtcaactaca gccagatcga tccggcgtta tgccgtgaac tctttattcg
    33481 ccatgcgctg gtggaaggtg actggcagac gcgtcacgca ttcttccgtg aaaacctgaa
    33541 actgcgggcc gaagtggaag agctggaaca caaatcacgt cgccgcgata ttctggttga
    33601 tgacgaaacg ttgtttgagt tctacgacca gcgcatcagc cacgatgtga tctctgcacg
    33661 ccacttcgac agctggtgga aaaaagtcag ccgcgaaacc cctgatttgc tcaactttga
    33721 aaaaagcatg ttgatcaaag agggcgcaga aaaaatcagc aagctggatt acccgaactt
    33781 ctggcatcag ggcaatctca agctgcgttt gagctatcag tttgagcccg gcgcggatgc
    33841 tgacggtgtg accgtacata ttccgctgcc gttacttaac caggttgagg aaagcgggtt
    33901 tgaatggcag atccccggtc tgcgccgcga actggtgatt gctctgatta aatcgttgcc
    33961 gaaaccggta cgccgtaatt ttgtacccgc accaaactac gccgaagcgt ttttaggccg
    34021 cgtcacaccg ctggagttac cgttgctcga cagccttgag cgcgagttac ggcggatgac
    34081 cggtgttacc gttgaccgcg aagactggca ctgggatcag gtgcccgatc acctgaaaat
    34141 caccttccgc gtggtggacg acaaaaataa gaagctaaaa gaagggcgct cattacagga
    34201 tctgaaagat gcgctgaaag gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg
    34261 tatcgagcag agcgggctgc atatctggag ctttgggcaa ctgccggaaa gctacgaaca
    34321 gaagcgcggc aactataaag tgaaggcgtg gccggcgctg gtggatgaac gtgacagtgt
    34381 ggcgatcaaa ctgtttgata acccgctgga gcaaaaacag gcaatgtgga acggtcttcg
    34441 ccgtctactg ctgctaaata ttccctcgcc gatcaagtat ttacatgaga agttaccaaa
    34501 caaagccaag cttggactgt actttaaccc gtatggcaaa gtgctggagc tgatcgacga
    34561 ctgtatctcc tgcggtgtgg atcaactgat cgacgccaat ggtggcccgg tctggacgga
    34621 agaaggcttt gctgcgctgc atgagaaagt gcgtgccgaa ctgaacgaca cggtggtaga
    34681 tattgcgaag caggtcgagc aaattcttac ggcagtgttc aatatcaaca aacgtctgaa
    34741 agggcgggtg gatatgacca tggcactggg gctttctgac attaaagcgc agatgggcgg
    34801 gttggtatat cgcggatttg tcactggtaa cggcttcaaa cggctgggcg acacgctgcg
    34861 atatttgcag gcgattgaaa aacggctgga aaaactggcg gttgatccac atcgcgaccg
    34921 tgctcagatg ctgaaagtcg aaaacgtcca gcaggcgtgg cagcaatgga tcaacaaact
    34981 gccgcctgca cgtcgtgagg atgaagacgt gaaagagatc cgttggatga tagaagagtt
    35041 gcgcgttagt tacttcgctc aacaacttgg tacgccttat ccgatttcag ataagcgtat
    35101 tttgcaggcg atggagcaga ttagcggtta accctgctat ttgcctgata aagaaaaaac
    35161 acggtaagca tttagcgccg ggttttttat taattctaaa acggtaaggg taaaaattca
    35221 ggaattcaga aaaatacaat tctctgctgc aagatgaata atgtttatct acagcatttc
    35281 cttaaaagat atgtcaggct tgcggagtgg cggttaagga catacgactt cctcctttca
    35341 gagtgctccg cttctcacta ttatctcacg cagtattctt aagggaacga taaggaggaa
    35401 ccatgaacat taccccgttt ccgacgcttt cgccggcaac tatagatgcc ataaatgtta
    35461 tcggacagtg gctggcgcag gatgatttct ccggtgaggt gccgtatcag gccgattgcg
    35521 tgatccttgc aggcaatgcg gttatgccga ctatcgacgc ggcatgtaag attgctcgcg
    35581 atcagcaaat tcctttactg attagtggtg gtatcggtca ctcgacaact tttttgtata
    35641 gcgccatcgc acagcatccg cactacaaca ctatccgcac cactggcaga gcagaagcga
    35701 ccatcctggc ggatatcgct catcagttct ggcacattcc gcatgaaaaa atctggattg
    35761 aagaccagtc aacaaactgc ggtgaaaacg cacgctttag catcgcgcta ttgaaacagg
    35821 ccgtagaacg agttcatacg gctatcgttg ttcaggaccc caccatgcag cggcgcacga
    35881 tggcgacgtt ccgccgtatg actggggaca atcccgatgc accacgctgg ttaagttatc
    35941 ccggattcgt tcctcagtta ggaaataacg cagacagtgt aatctttatt aatcagttac
    36001 aaggattatg gccggttgag cgttatctct cactactcac tggcgagctg ccgcgtttac
    36061 gcgatgatag cgatggctac ggtccccgcg ggcgagattt tatcgttcac gttgattttc
    36121 cggcagaagt catccatgca tggcaaacgc tgaaacatga tgcggtgctc atcgaggcga
    36181 tggaaagtcg ctcgttacgt taaaaattgc ccgtttgtga accacttgtt tgcaaacggg
    36241 catgactcct gacttttatt tctgcctttt attcctttta cacttgtttt tatgaagccc
    36301 ttcacagaat tgtcctttca cgatgccgtc tctctgatga ttgatgttaa ttaacaatgc
    36361 attcaccgaa aataaacata taaatcacag gagtcgccca tgtcagtacc cgttcaacat
    36421 cctatgtata tcgatggaca gtttgttacc tggcgtggag acgcatggat tgatgtggta
    36481 aaccctgcta cagaggctgt catttcccgc atacccgatg gtcaggccga ggatgcccgt
    36541 aaggcaatcg atgcagcaga acgtgcacaa ccagaatggg aagcgttgcc tgctattgaa
    36601 cgcgccagtt ggttgcgcaa aatctccgcc gggatccgcg aacgcgccag tgaaatcagt
    36661 gcgctgattg ttgaagaagg gggcaagatc cagcagctgg ctgaagtcga agtggctttt
    36721 actgccgact atatcgatta catggcggag tgggcacggc gttacgaggg cgagattatt
    36781 caaagcgatc gtccaggaga aaatattctt ttgtttaaac gtgcgcttgg tgtgactacc
    36841 ggcattctgc cgtggaactt cccgttcttc ctcattgccc gcaaaatggc tcccgctctt
    36901 ttgaccggta ataccatcgt cattaaacct agtgaattta cgccaaacaa tgcgattgca
    36961 ttcgccaaaa tcgtcgatga aataggcctt ccgcgcggcg tgtttaacct tgtactgggg
    37021 cgtggtgaaa ccgttgggca agaactggcg ggtaacccaa aggtcgcaat ggtcagtatg
    37081 acaggcagcg tctctgcagg tgagaagatc atggcgactg cggcgaaaaa catcaccaaa
    37141 gtgtgtctgg aattgggggg taaagcacca gctatcgtaa tggacgatgc cgatcttgaa
    37201 ctggcagtca aagccatcgt tgattcacgc gtcattaata gtgggcaagt gtgtaactgt
    37261 gcagaacgtg tttatgtaca gaaaggcatt tatgatcagt tcgtcaatcg gctgggtgaa
    37321 gcgatgcagg cggttcaatt tggtaacccc gctgaacgca acgacattgc gatggggccg
    37381 ttgattaacg ccgcggcgct ggaaagggtt gagcaaaaag tggcgcgcgc agtagaagaa
    37441 ggggcgagag tggcgttggg tggcaaagcg gtagagggga aaggatatta ttatccgccg
    37501 acattgctgc tggatgttcg ccaggaaatg tcgattatgc atgaggaaac ctttggcccg
    37561 gttctgccgg tggtcgcatt tgacacgctg gaagaggcta tctcaatggc taatgacagt
    37621 gattacggcc tgacctcatc aatctatacc caaaatctga acgtcgcgat gaaagccatt
    37681 aaagggctga agtttggtga aacttacatc aaccgtgaaa acttcgaagc tatgcaaggc
    37741 ttccacgccg gatggcgtaa atccggtatt ggcggcgcag atggtaaaca tggcctgcat
    37801 gaatatctgc agacccaggt ggtttattta cagtcgtaat gagtaaaaga ggcggaggtt
    37861 ttttcctccg cctgtgcgcg tcagagttta gcgaattttt cgagggtgcg aataagctgc
    37921 gtgacgaagc catattcgtt atcgtaccag gcgaccgttt tcaccagttg taaatcgccc
    37981 acggcggtaa tttccgtttg cgtggcatca aacaccgaac cgaaatggct gccaatgata
    38041 tcggaagaga ctatttcttc atcggtataa ccaaatgact cgttattggt ggttgcttgt
    38101 ttaagtgcgt tattcacctc ttcggcagtc actttttttc cgagaatcga taccagttca
    38161 gtgaccgaac ctgttttcac cggcacgcgt tgcgcatgac ctttcagttt gccgctcagt
    38221 tccgggatca ccagaccaat ggcttttgcc gcccccgtag tgtggggaat gatattttct
    38281 gccgctgcgc gtgaagcacg taaatcttta ccacgtgggc catccaccag tgactgggtg
    38341 ccagtatagg catgaatggt cgtcatcgtg ccgacttcta tcccgaaact gtcatgcaag
    38401 gctttggcca tcggcgcaag acagttagtg gtgcatgacg ccacggaaac aatggtgtcg
    38461 ttgccatcca gagtgtcgtc attgacgtta taaacgatag ttttcatttc accggcaggg
    38521 gcggaaatca acaccttctt cgcaccagca tcaagatgcg cctgcgattt ctcggcggag
    38581 gtataaaagc cagtacattc gacaatgatt tctgcccctt tcgctttcca cggaatattt
    38641 ttagcctctt tttcggcgta aaccgcgata cttttcccat caacgataag tgaatcttcc
    38701 gtaaaatcaa cgctccaggg gaacggacca tagtttgaat catgtttcag cagataggcg
    38761 agaatttttg gggaagtgag atcattaata gcgacaacgt ctatgttgct tttgacttca
    38821 agtaatcgac gcaacaccag tcgaccgata cgaccaaaac cgttaatacc aactttactc
    38881 atggttttct cctgtcagga acgttcggat gaaaattgat cctttccagg cttagcccag
    38941 gattgtggga tgggcaatct ccattcttac agtgaaacgt aacgtactga aaacgggtga
    39001 acaatattta atgaaatttt gagaaaagcc cgttatgtta acggaaaatt atgttaaagc
    39061 aggaaatgtt atggaaaata aatactcaag gttacaaatc agcattcact ggctggtctt
    39121 tttgctggtt atcgcagcgt attgcgcaat ggagtttcgt ggtttcttcc cacgtagcga
    39181 tcggccactc atcaacatgg ttcatgtttc ctgtggtatc tcaattctcg tgctgatggt
    39241 ggttcgtctt ttgttaagac tgaaataccc aaccccgccg attataccta agccaaaacc
    39301 gatgatgacg ggactggcgc atttgggaca tttggtgatt tatctcctgt ttattgcgct
    39361 gccagtgatt ggtttggtga tgatgtataa ccggggcaac ccgtggtttg cgtttggttt
    39421 gacgatgcct tacgcttcag aggccaattt cgaacgggtt gatagcttaa agtcgtggca
    39481 tgagacgctg gcgaatctgg gatattttgt catcgggttg cacgctgcgg ctgcactggc
    39541 acaccactat ttctggaagg acaacacact tctacgcatg atgccgcgta aacgttcctg
    39601 aaggatattt aaagaaaacg cctgtactaa aaccgacccg tggtacaggc gaagaatacg
    39661 ggtctacatc ggaagcgcct attatattta tttgtatgac aaataaaacc ataatccttg
    39721 cccatacgtc catctggctt atttttaatc aattcacccg atctttgatc tcatcaacgg
    39781 tatcaaaata aaccagcgta taacgttcaa atcgaacata agattcgata aaccatggag
    39841 gttatatgaa aaaactggca cttattttgt ttatgggaac gcttgtttcc ttttatgccg
    39901 atgccgggcg caaaccctgt tctggttcga aaggggggat ctcacactgt acggcaggcg
    39961 gcaaatttgt ctgtaatgat ggttctatta gtgcctcgaa aaaaacatgc actaactgaa
    40021 gtgtaaaagg ggtgccatga gaaaatgaat ctgctagtca gatgcgcggg gaaaatcccc
    40081 gcgcttgccc ttacctggac gtgcaggcca tgagcgcagc aacctcctta tcaccgtccc
    40141 ggaaccgcag ttcgtagagc gtttgtcggg tcaggagtgt gaatgtcaga atcgtaatgc
    40201 agataatgag cagacacacc accagagggt tgtgcttcat agcctttctc cttgccggat
    40261 ggcgggtaag aggctaagat ctgaattgct aggttcattc gttggcctcg gttgatagaa
    40321 atatcggtcg gggccttcgt ctttctgatt cccggttagc ctgaaaacag aaagtctcag
    40381 gcacccgcag gcatcctatg aggtttcctt agggacgaaa ataatcactt cacgaaattg
    40441 cgtgctgttt tccagaattt ttcgtcattc ggattagcca gtttagccat tcgttactct
    40501 cttcattcca atagcattaa ttttctctgc aataattgtt gtaaaaatgt gacgcaaaga
    40561 ggtttttggt cataagtaat taccgtcaag tgccgatgac tttctatcag gagtaaacct
    40621 ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt tttgcatcac
    40681 atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct attcgcatta
    40741 agttctgctc tggctggcta tttcctctgg caggccgatc gcgatcagcg tgatgttact
    40801 gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg
    40861 atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcga
    40921 aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aagggtatcg tgcttatcag
    40981 aatcggccgg tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa tcaacgcttt
    41041 caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa
    41101 gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt
    41161 ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga actggcccat
    41221 cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcttgc cctggtcatg
    41281 acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca
    41341 caacggattg aaaaaatcgc cagtggcgat ctgacgatga atgatgaacc ggcgggtcgt
    41401 aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact ggggatgaca
    41461 gtagggactg ttcgacaggg tgcggaagag atttatcgtg gcaccagcga aatttcagct
    41521 ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggcaatcga acaaactgcc
    41581 gccagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc
    41641 agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggtttccggt
    41701 gtagtaaaaa cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc
    41761 gtcatcaaca gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc
    41821 gcgcgagcgg gtgagcaagg ccgtggattt gccgttgtcg ccagcgaagt acggacactc
    41881 gcaagccgca gcgcccaagc ggcgaaagag attgaaggct tgatcagtga atcagtcagg
    41941 ttaattgacc tggggtcgga tgaggtggca acggcaggga aaaccatgag cactattgtt
    42001 gatgccgtcg cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa
    42061 agtagaggca taacgcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag
    42121 aatgcttctc tggtagaaga ggcctcagcg gcggcggtgt cccttgaaga acaggcggca
    42181 cgattaactg aggcggtgga tgtattccgt ctgcacaaac attctgtgtc ggcagaacct
    42241 cgcggagcgg gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga
    42301 cagatccctt cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc
    42361 aaccagaaga gaacgtcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat
    42421 gtcccggaac aggcagggcc accagcgtgg catgacgcag gtcgtctttt accgcgccag
    42481 aagggacaaa ccacacgtag tcgtattcaa ccgtaagttg acgagatagc gaagcagaca
    42541 gcgtttcgat acaacccgaa ggaattttac atccctggct ctgtactaat gcatctgaat
    42601 gctggcgtgg cgcagtgcct tctggtgata caacgaccgg ccattccagc actcggctta
    42661 gcgttacgtt ctcctgaagt agcgggtgat tagggcggac aaccagcttc agcgattcaa
    42721 gaaacagcag ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc
    42781 cgatatcgat ttccccggtt tttaaacccg ccagaatcat agggttactc attgtcgcaa
    42841 cttgcaaggt cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc
    42901 ccagtgccgc agtaggtagt gcaccaaccc tgacgacatc attattaaga tcttctttac
    42961 gatgaagcga ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa
    43021 attgttcgcc gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc
    43081 cagtcagctg ctccagttca ttcaatgtct tagagagcgc aggttgactc aaattaaggg
    43141 tttcagccgc gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc
    43201 gcaaacggat gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag
    43261 cggtgtcgct gacaagtgaa gttgtttgat tatgataa
//
LOCUS       DAPHDX010000025        42704 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPHDX010000025 DAPHDX010000000
VERSION     DAPHDX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.025"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..964)
                     /locus_tag="RH058_001324"
     CDS             complement(<1..964)
                     /locus_tag="RH058_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HDV3136931.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRH"
     gene            complement(1212..1787)
                     /gene="nfuA"
                     /locus_tag="RH058_001325"
     CDS             complement(1212..1787)
                     /gene="nfuA"
                     /locus_tag="RH058_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV3136932.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(1846..2529)
                     /gene="gntX"
                     /locus_tag="RH058_001326"
     CDS             complement(1846..2529)
                     /gene="gntX"
                     /locus_tag="RH058_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV3136933.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            2567..3337
                     /gene="bioH"
                     /locus_tag="RH058_001327"
     CDS             2567..3337
                     /gene="bioH"
                     /locus_tag="RH058_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV3136934.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(3366..4244)
                     /gene="rpnA"
                     /locus_tag="RH058_001328"
                     /pseudo
     CDS             complement(3366..4244)
                     /gene="rpnA"
                     /locus_tag="RH058_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            complement(4447..4683)
                     /gene="feoC"
                     /locus_tag="RH058_001329"
     CDS             complement(4447..4683)
                     /gene="feoC"
                     /locus_tag="RH058_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV3136935.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(4683..7004)
                     /gene="feoB"
                     /locus_tag="RH058_001330"
     CDS             complement(4683..7004)
                     /gene="feoB"
                     /locus_tag="RH058_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV3136936.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(7021..7248)
                     /gene="feoA"
                     /locus_tag="RH058_001331"
     CDS             complement(7021..7248)
                     /gene="feoA"
                     /locus_tag="RH058_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV3136937.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(7686..10007)
                     /locus_tag="RH058_001332"
     CDS             complement(7686..10007)
                     /locus_tag="RH058_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV3136938.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(10104..10580)
                     /gene="greB"
                     /locus_tag="RH058_001333"
     CDS             complement(10104..10580)
                     /gene="greB"
                     /locus_tag="RH058_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV3136939.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            10808..11527
                     /gene="ompR"
                     /locus_tag="RH058_001334"
     CDS             10808..11527
                     /gene="ompR"
                     /locus_tag="RH058_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV3136940.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            11524..12876
                     /gene="envZ"
                     /locus_tag="RH058_001335"
     CDS             11524..12876
                     /gene="envZ"
                     /locus_tag="RH058_001335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV3136941.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(12952..14574)
                     /gene="pckA"
                     /locus_tag="RH058_001336"
     CDS             complement(12952..14574)
                     /gene="pckA"
                     /locus_tag="RH058_001336"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV3136942.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            14953..16677
                     /locus_tag="RH058_001337"
     CDS             14953..16677
                     /locus_tag="RH058_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV3136943.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLAAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(16740..17618)
                     /gene="hslO"
                     /locus_tag="RH058_001338"
     CDS             complement(16740..17618)
                     /gene="hslO"
                     /locus_tag="RH058_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV3136944.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMSLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVKFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(17643..18044)
                     /gene="hslR"
                     /locus_tag="RH058_001339"
     CDS             complement(17643..18044)
                     /gene="hslR"
                     /locus_tag="RH058_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV3136945.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(18055..18723)
                     /gene="yrfG"
                     /locus_tag="RH058_001340"
     CDS             complement(18055..18723)
                     /gene="yrfG"
                     /locus_tag="RH058_001340"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV3136946.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(18788..20923)
                     /gene="igaA"
                     /locus_tag="RH058_001341"
     CDS             complement(18788..20923)
                     /gene="igaA"
                     /locus_tag="RH058_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV3136947.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21243..21803
                     /gene="nudE"
                     /locus_tag="RH058_001342"
     CDS             21243..21803
                     /gene="nudE"
                     /locus_tag="RH058_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV3136948.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLVGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(21968..24520)
                     /gene="mrcA"
                     /locus_tag="RH058_001343"
     CDS             complement(21968..24520)
                     /gene="mrcA"
                     /locus_tag="RH058_001343"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV3136949.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            24640..25419
                     /gene="hofM"
                     /locus_tag="RH058_001344"
     CDS             24640..25419
                     /gene="hofM"
                     /locus_tag="RH058_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV3136950.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            25419..25958
                     /gene="hofN"
                     /locus_tag="RH058_001345"
     CDS             25419..25958
                     /gene="hofN"
                     /locus_tag="RH058_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV3136951.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            25942..26382
                     /gene="hofO"
                     /locus_tag="RH058_001346"
     CDS             25942..26382
                     /gene="hofO"
                     /locus_tag="RH058_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV3136952.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            26372..26776
                     /gene="hofP"
                     /locus_tag="RH058_001347"
     CDS             26372..26776
                     /gene="hofP"
                     /locus_tag="RH058_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV3136953.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            26688..27926
                     /gene="hofQ"
                     /locus_tag="RH058_001348"
     CDS             26688..27926
                     /gene="hofQ"
                     /locus_tag="RH058_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV3136954.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            28327..28848
                     /gene="aroK"
                     /locus_tag="RH058_001349"
     CDS             28327..28848
                     /gene="aroK"
                     /locus_tag="RH058_001349"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV3136955.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            28905..29993
                     /gene="aroB"
                     /locus_tag="RH058_001350"
     CDS             28905..29993
                     /gene="aroB"
                     /locus_tag="RH058_001350"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV3136956.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            30085..31371
                     /gene="damX"
                     /locus_tag="RH058_001351"
     CDS             30085..31371
                     /gene="damX"
                     /locus_tag="RH058_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV3136957.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQPLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPVAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            31478..32314
                     /gene="dam"
                     /locus_tag="RH058_001352"
     CDS             31478..32314
                     /gene="dam"
                     /locus_tag="RH058_001352"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV3136958.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            32332..33009
                     /gene="rpe"
                     /locus_tag="RH058_001353"
     CDS             32332..33009
                     /gene="rpe"
                     /locus_tag="RH058_001353"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV3136959.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALATGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            33002..33760
                     /gene="gph"
                     /locus_tag="RH058_001354"
     CDS             33002..33760
                     /gene="gph"
                     /locus_tag="RH058_001354"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV3136960.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            33753..34757
                     /gene="trpS"
                     /locus_tag="RH058_001355"
     CDS             33753..34757
                     /gene="trpS"
                     /locus_tag="RH058_001355"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV3136961.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            35026..35931
                     /locus_tag="RH058_001356"
     CDS             35026..35931
                     /locus_tag="RH058_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3136962.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            35948..36310
                     /locus_tag="RH058_001357"
     CDS             35948..36310
                     /locus_tag="RH058_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV3136963.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            36394..37557
                     /locus_tag="RH058_001358"
     CDS             36394..37557
                     /locus_tag="RH058_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV3136964.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            37557..38783
                     /locus_tag="RH058_001359"
     CDS             37557..38783
                     /locus_tag="RH058_001359"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV3136965.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            38780..39658
                     /locus_tag="RH058_001360"
     CDS             38780..39658
                     /locus_tag="RH058_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV3136966.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            39669..40022
                     /locus_tag="RH058_001361"
     CDS             39669..40022
                     /locus_tag="RH058_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV3136967.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            40034..41338
                     /locus_tag="RH058_001362"
     CDS             40034..41338
                     /locus_tag="RH058_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV3136968.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            41350..42435
                     /locus_tag="RH058_001363"
     CDS             41350..42435
                     /locus_tag="RH058_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3136969.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(42542..>42704)
                     /locus_tag="RH058_001364"
     CDS             complement(42542..>42704)
                     /locus_tag="RH058_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV3136970.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT         9881 a        10817 c        11743 g        10263 t
ORIGIN      
        1 tatgccgtga cgcatcgaac ggcacgcctg acttcagcac tccatacgcc acctgtgcca
       61 gcttgcgcat catcgcgccg agaatcacct ttcctttctt gccattagcc gccagacggt
      121 cgcggaacgc ccgtccccac tcagtcttac tggtggctac cattgcgggc atatacaacg
      181 ccctgcgaag cgacacatgt ccggccttac tcatccggct cgcccctctc acactgctac
      241 ctgattcata acgccgtggt gtcagacccg caaaagcggc gaactgtctg gcatgggcga
      301 agcggtcctt cagaccgata taagccagca ataccgcaga tgttttctct ccgatacccg
      361 ggatgctttc cagcagtttc ctgcggtgtt tcatatccgg atcatcgtct gtcaggtctt
      421 ttatctgctt ctcaagacgc ttcagctctg cttcaagcca cagaaggtga gcatcaatgc
      481 tcggtctctg gacttcccgc gccgtttcag tgcgattcag ttcctgcgtg tgcatatctg
      541 tcagcgcctg gtggcggact accagggcac gcaacgcgcg ttcaagcggg tgaggcgctt
      601 cccaggctgc agggcgcttc tgacgacaga actctgccag catgcgcgca tccacggtat
      661 cagtcttgtt acgcagtcct tcactctgag cgaaagcttt acccagcgca ggattaatga
      721 ctgacactat gtagccagca tcgtaaaggc actcagcgac aggttccata taggtgccgg
      781 tcgcttcgat gcagatatgc gcatggtcaa tcttgtgacc tttcagccag ctcaccagct
      841 catcgtgccc tttagtggtg ttagcgaatt ttttggtgcg atgacgacca tcaggacgca
      901 acacatcgac atccagtttc tctttagcgg tgtcgatacc gatataatga agttcatgtt
      961 ccatagtgaa accaaccttg caaatacgga ttaccgggaa aaccggtcca tgatactgtc
     1021 cggtttatca ctttgggaga aaggcagtcc gctgcataaa tctacgcaac aggttagaac
     1081 ccaaggcccg atacggcatg cggactgcag gcagagaaaa ctggccgatt ttctctgcac
     1141 tggagggata atacaaggcc cgaaatgacg ctttagcgtc gcatcgggca atctacaaaa
     1201 gaggggataa cttagtagta ggagtgttcg ccgcgctggt gttcggtgag atcgcgcaca
     1261 cctttcagct ccgggaattc gttcagcagc tgcttctcga tcccttcttt cagcgtcaca
     1321 tcgaccatgg aacaaccgtt acagccgccg ccaaattgca gaatggcgta accgtcttcg
     1381 gtgatttcca tcagcgaaac gcgaccaccg tgaccagcaa gctgtgggtt gatctgcgac
     1441 tgcagcatat actccacgcg ctccatcagc ggtgcatcgt ctgccacttt acgcattttg
     1501 gcgttcgggg ctttcagcgt taactgagaa cccaactggt cggtaacaaa gtcgatctct
     1561 gcatcttcca ggtatggtgc gcttaactca tcaacatacg cggtcagcag gtcaaatttc
     1621 agggctgtgt cggtggcttc cacagcgtcc ggcggacaat aagaaacgcc acattcagcg
     1681 ttaggcgtgc cagggttaat cacaaatacg cggatttgtg tcccttcttc ctgatttgcc
     1741 agcagtttgg caaagtgcgc ttgtgcagca tcggaaatac ggatcatagt aatggcctaa
     1801 tagttgacta ttttagttgg ttataatacg cccatcatcg aggctctaca aggttcgaca
     1861 aaggcaccag acctggacag ccgccgcacc attgcgtaaa agcaactgcg caatctctgc
     1921 gacggtactt ccggtggtaa cgacatcatc cacaatcacc atatggcgac cttgcacggg
     1981 caattcaaga cgaaaggcat ttttcaggtt gcgcttgcgc agccgggcac tgagaaaatg
     2041 ctgggtcgca gtggcccgtg tacgtgtgac ggcttcgcta tcccattggc agtgcaacca
     2101 gcgtgataac ggctgacaca gcaaatcgct ctgattaaat ccccgacgcc agtgacgccg
     2161 ctgccataac ggaacgctga cgatgcgatc cggcaattgc aacccggtgg tgcgacgagc
     2221 gtgtaagact tccaatagta acagacgtga cagggcgctg gcgatttcac tgcgccggga
     2281 aaatttaagc tggtggataa gcggacttaa cggcggcgca tagtcggcaa ccgtgaccag
     2341 tctttgccag ggcggcggtt tttgcaggca gcgaccgcag ggaagatggg agtgtgtggc
     2401 gggtaatcca cattgtgggc ataacgtttt atctgtgcgg gtggcgcgtg aacagaccga
     2461 acaaatcccc caatgaccta acgccagtgg cattcggcat agccagcata atcccggtac
     2521 tgttagcata tgttcatcct tgtaagtcaa aagagaacaa tagcggatga ataacatctg
     2581 gtggcagacc aaaggtcagg ggaatgttca tcttgtgctg ctgcacggat ggggactgaa
     2641 tgccgaagtg tggcgttgca ttgacgagga acttagctcg cattttacgc tgcaccttgt
     2701 tgacctgccc ggcttcgggc gtagccgggg atttggtgcg ctgtcacttg ctgatatggc
     2761 cgaagccgtg ctgcaacagg cacctgataa agccatttgg ttaggctgga gtctgggcgg
     2821 gctggtggca agccagattg cgttaaccca tcccgagcgt gttcaggcgc tggtcaccgt
     2881 ggcgtcgtca ccttgtttta gtgctcgtga cgagtggccg gggataaaac cggacgtgct
     2941 ggcgggattt cagcagcaac tcagtgatga ttttcagcgt acagtggagc ggttcctggc
     3001 gttacaaacc atggggactg aaacggcgcg ccaggatgcg cgggcgttga agaaaaccgt
     3061 tctggcgtta ccgatgccgg aggttgacgt gcttaatggc gggctggaaa tcctgaaaac
     3121 ggtcgatctc cgtcagccgc tgcaaaacgt gtccatgccg tttttgcgat tgtatggcta
     3181 tctcgacggt ctggtgccgc gcaaagtggt gccgatgctg gataaacttt ggcctcacag
     3241 cgaatcatat atcttcgcca aagcggccca tgcgccgttt atttcgcatc cggtcgagtt
     3301 ttgtcacctg ctggtggcgt tgaagcagag ggtgtaggca acttttgaaa tggcgagaca
     3361 gaaatttact ggctcgccgc agccaactct tcttctgaca ccccagtaaa gcgcatgatg
     3421 tctgtaagag ggactccgga ttcaagcatt attttggcta tatgcagggc tttggattgt
     3481 tccccctcct gccgcaatct ttccgcaatt gtcattaaac tctccttgtg tttcggtgaa
     3541 cgttcggcaa cgccgtcgat aaaatcgtta aaacgtaccg cgtcgccggt ttgcagtatg
     3601 taattaaaca gccctttgat ttgtctgtca ttagcgtatc cactacttaa taagcaggcc
     3661 atttgctcta ccagccccat caggtcgcgt tgacgaatat gtttttgaat taactccagc
     3721 agcgccatac gtcggtgctg catgatttca tcatcaggca tgacggtgac atcaatcagc
     3781 ggaaatgcgg aggcataaag ctgccttgcc agtttgggat cggcgaaaca atccagccaa
     3841 cacagcgaat agggataggg gctttcaata ccgtggtaaa acaacaatag caccaccatc
     3901 ggcaacgttt tgtatccagc atcaaggtga ttttgcattg cggcaatagc ttaacgcatc
     3961 atgcgaaatg cgatcagttt gtttgaggtg ctttgatgtt caatcagaca atagatgtat
     4021 cctggtcctt gttccgtttt caccgaccac agcacatcgg aatagctttc acgcagatcg
     4081 tcatcaataa agctgctcga ctccagcttg agggttttca tatcacagag cgcgtgaatg
     4141 ggggccggta aatggaacgc aagaaaatcg cgagccgtgt ccggttggcg taaaaagagt
     4201 ttgaacagcg catcatgtgg ggtggaactc tgctttttac tcatggcgtt ccttcacctg
     4261 ttgtttgaat gccgcgactc tacgcgccac tccacatctg tgcattagcg agttttcatc
     4321 cctgcgagat gctttcaaag gaacttacgc cgtgctgcaa gagttgcatt tttatggaaa
     4381 gccgcacgca aaaaagcccg gatgcagacg cataccgggc tttccaaaac aggcgatgga
     4441 gtaaggttaa cgcagcgccc accactcgcg cagacaggct tttccttccg ggcagctttt
     4501 acaactgcca gagaggcagc cgtcaggttc ttcctgaatc cgcacggctt tgcccatact
     4561 ttccagttgt tgcagcatgg cgttaatcat tggctgtgga gtgttcaatg tctggcttat
     4621 ctgggccgct tccatacggc cccgtaacgc cagcaaatcg cgcacctgaa taagtgaagc
     4681 cattaatggc aatcaccggt ggtgctggct gcgcagcaac tgcttaccga cttgcgggtt
     4741 gccagcagtt cgatatccac ccggctacgc gcgcggcgca gcagaccgat aaccacgatg
     4801 ttaaacagga taaccgccag aatgcacacc aggctgtaag ttgggtgctg actgtagctg
     4861 gcgacctgat agaacaatgt tgccagcgag taagcgatat tcagccccca caggatggag
     4921 aagcccatcc agccacggct tgattcacgg gcgatggctc ccatcaccga gatacatggt
     4981 acatacagca ggacgaaaat caggtagctg taagctgctg ctgcgctacc gaatttctga
     5041 tccatcacgc ccatcgcccc ggtacccatt tcgccgtcgc ctttgctggc ttcaatgggg
     5101 ttcatcagta cgctaaggct gaaggtgtct ttcaggctct gccaggtttc atctatcgca
     5161 ctgaacagct cttcaccgag gttaaactct gccggattga actcttcgtc ctgaatattt
     5221 tctgcggtgt agagggtgtt gagcgtaccc actaccactt ctttcgccat ggcacctgta
     5281 aacaggccaa ccgttgcctg ccagttatct tcatgcacgc caattggctt gaagaccggg
     5341 gtgatcaccc ggctgacgga cgccagcgcc gagtcgttga tgttatcgac gattttcccg
     5401 ctcagcgaga agctgttgaa agcgctcagg aaaatgctga cgatgatgat cactttacca
     5461 gcacgcagaa cgaagccttt cagacgctgc caggtctgga taatcaggct tttaacgtgt
     5521 ggtacatgat agaccggcaa ctccatgaca aacggcgtcg cttcaccgcg catgatggtg
     5581 tacttgagca tcaggccagt cagcaccgcc atcacaatac ccagcatata cagcgagaag
     5641 accgccagcg caccgttctg cccgaagaag gcagccgcga atactgcgaa gatagccaga
     5701 cgcgcgccgc aggacataaa cggtgccatc atgatggtca tcagacgttc acgcggtgca
     5761 tcaagcgtac gtgcacccat taccgacggt acgttacaac cgaaaccgac gatcagcggc
     5821 acaaaggatt tccccggcaa gcccagcgcc tgcatcagac ggtccatcac aaacgccgca
     5881 cgcgccatat acccggagtc ctcaaggaag gagaggaaca ggtacatcat gccaatctgc
     5941 ggcaccagtg gcagcacggt gttaataccg ccacccaggc cctgggcgag gaagatagtc
     6001 agccagtccg ggaagtggag cgtgtagcca atccattgaa taccatgcac aaatagcgcc
     6061 acggagccga cgtcaaacag cggctgtaac gccccgccga tgttgatagc cagcaggaac
     6121 atcaggtaca tcacaaagag gaaaatcggc agaccgagga aacggttgag cacgatttta
     6181 tctaccgcag tggtgaaacg gctgggttct gccgtcaggg tgttgcttac cacatcacag
     6241 atggcagcaa tgcactggta acgcgcatcg gcaatgtgca gcgccggatc gtccatctca
     6301 ttacgcagac gggcgagggc ggcatccaga tgctgcgacg cttcaccggc gtaggcgcgg
     6361 ctgtagatat cgccttccag catttgcagg cccagccagc gacgttgttt cagcgggatg
     6421 tcggaaggca tcacttttgc cagtgaatct gcttcgttga gtagcggctg tgcgtaatgc
     6481 accagttcca cattctcgtt agctttatag cgatcaatcg ccagcttgag cgcttcaata
     6541 ccgcgaccac gggttgaaac cagcgggatc actgggcagc caagacgcgc cgacagagca
     6601 tcaatttcaa tacgaatatt ttgcttctcg gcaatgtcga gcatgttcag tgccacgatg
     6661 cagggaatgc cgagttccag cagttgtagc gtcaggtaca ggttacgctc aaggttagac
     6721 gcatccacca cgttaatcag caggtcggcg tcgccactca aaatgtagtg acaggcgatt
     6781 tgctcatcga gcgaggtctg cgatgagatg gtggtcagag aataggtgcc gggcaggtcc
     6841 accagcgtga cctgatgatc ggtggtggag aattgccctt ctttacgttc gacggtaacg
     6901 ccagcccagt tacctacacg ctgacgtgcg ccagtgagct ggttaaataa ggttgtcttg
     6961 ccagaatttg gattaccaat taagcctatg gttaattttt tcattgttgt tatcaccgta
     7021 ttaacaggaa accgcttcca cttctaataa ggccagatct tttttgcgta ataccaggct
     7081 cacacgacgg gtttcgatat gaatggggtc gccgagtgga gcgacgcgca ccacattaaa
     7141 agaggagcca ggtaacatgc caagagaaag cagtttttgg cgatatgccg ggctgatttc
     7201 acgggaaaag ccagtgattt tccacgcagt atctggagtg tattgcatag gtgcctactt
     7261 gtttctcatt aactggataa ctacctcatg gggcgtttga cagcgacatg agaccagatg
     7321 atttgccaac aaaggagaga ttaaataatc aacaacacaa tgataatgag aatggtttct
     7381 atcagcaata cttaaaatgt gtgcgaatgt tggtttgaat aataatctga ctcaatgttg
     7441 atgtggctca aggttatatc gcagcgaaaa tataattcga atagcgaatt accaggactg
     7501 gctaatgttt aattaaggtt tcagttgtta atgaagtgat aaatgttgcc ggaatatatt
     7561 gtgagtttgg ctgtggaata aaaatgccgg agatattctc cggcactttt atgtcagaca
     7621 aggcgttcgt tgccagatgc ggcgtggaca ccttatcgcg caggtttagg ggtcaatcag
     7681 ggcgtttaac gttttttgcc cattgctgcc gccagcgcgt ccatcatcgc gctgttaccg
     7741 gcaggctgcg cttcacgtcc gcgcggtttg gctgccgggc ggttgttttg cgggcgttca
     7801 ttaccgccgc cgcgacgggc gttggtttcg ccaggctgct cgtccagacg catggtcagg
     7861 gcgatacgtt tacgctgaag atccacttcc agcactttca ccttcacaat gtcgcccgct
     7921 ttcaccacgg tatgcggatc ttctacaaac ttgttcgaca atgaagagat gtgaaccagg
     7981 ccgtcctgat gcacgccgat atcgacaaac gcgccaaagt tggtgacgtt ggtcaccgca
     8041 ccttcgagga tcatacccgg ttgcaggtcg ttcattgtct cgacgccatc ggcaaactga
     8101 gcggttttaa attccggacg cggatcgcga cccggttttt ccagctcttt gatgatgtca
     8161 gttactgtcg gcacaccgaa tttttcatca gtaaagtcag acgctttcag gttacgcagt
     8221 tcgctgctgt tacccatcag atctttcagt gcctgctgtg ttgctgccag aatgcgttcc
     8281 accaccggat aggcttccgg gtggacggta gacgcgtcca gcgggttatc accatggtta
     8341 atgcgcaaga agcccgcgca ctgctcgaag gctttcggcc ccagacggct cactttcagc
     8401 agttgctgac ggttctggaa ctgaccgttc tcatcgcgcc aggccacgat gttttgcgcc
     8461 atcatgcgcg tcaggcccgc cacgcgggtt aacagcggaa cagaagcagt gttgagatcg
     8521 acgccaacgg cgtttacgca gtcttctact actgcgtcca gtttgcgggc cagttgcgtc
     8581 tggctgacgt catgctgata ctgacctacg ccgatagatt tcggatcgat tttcaccagc
     8641 tccgccagcg gatcctgcaa acggcgggcg atagacaccg cgccacgcag cgaaacgtcg
     8701 agatccggaa actcctgcgc tgccagctcg gaagccgagt aaaccgacgc gccagcttcg
     8761 ctgacgatca ctttctgtgc ggtcactttc gggaactgct tctgcacgtc gagatagaaa
     8821 cgttcggttt cgcgggaagc tgtaccgttg ccgatcgcca ccagttcaac gttatgcttt
     8881 tcacacagcg cagcaacagt catcgctgct tttgcggctt gtccggtgtg cgggtaaatg
     8941 gtatcggtcg ctaccagttt gccagtggca tcgaccaccg ccacttttac cccagtacgc
     9001 agacccggat cgaggcccat cgttgcacgc agtccggcag gggccgccat cagcagatcg
     9061 tgcaggttac gggcaaagac gttgattgct tcatcttccg cgcgttcgcg cacggtaccc
     9121 atcagttcgg tttccagatg catcagcacc ttgatgcgcc aggtccagct cactacgcct
     9181 ttgcgccagc tatccgccgg ggcattgttc aggcgcaggc caaggtgatc catgatgatt
     9241 tgctcgcaat agctctcttt gggcggctca tcgaactgcg gatcggcatt cagcgaaagc
     9301 tggagtacgc cttcgttacg cccacggaac atcgccagcg cgcggtgaga aggcaccgtg
     9361 gacaacggtt cgtgatgatc gaaatagtcg cggaattttg ccccttcctc ttctttaccg
     9421 ctcaccacgg ttgagaccag atgcgcgttc ttccacagat aatcacgcac tttcgccagc
     9481 agcgcggcat cttcggcaaa acgttccatc aggatatagc gcgcgccgtc cagcgcggct
     9541 ttggtatctg ccacgccttt atcggcatca acatattgtg cagcggcgac ttctggcgtg
     9601 tgtgacggat cgctccacag caggtcagcc aacggctcaa gccctgcttc aatggcgatt
     9661 tgcccgcggg tgcggcgttt aggtttgtag ggcaggtaga ggtcttcgag ttcggttttg
     9721 cttagggtgg cgttgatggc cttcgccaga tcatcggtga gtttgccttg ctcggaaatg
     9781 gatttgagga tcgcctgacg tctctcttcc agctcgcgca gatagctcag acgcgtttcc
     9841 agattacgca gctgcgtgtc atccagaccg ccggtgattt ccttacgata acgtgcgata
     9901 aacggcacgg tattcccttc gtcaagcagg cgaacggcag cgtcaacctg ttccgggcgc
     9961 gcctgaattt cacccgcaat aatgcggcag aacgaatcat tcatcatggt ttgagttcat
    10021 cttttcggat caaaaatcag gggatagtta tacggactgg ctggcaaaaa tgccagccat
    10081 cggcaggagg ttaagactct tccttacggt ttcacgtact cgatagcatt aacataccag
    10141 ctcgcttccc cggcaggggt attcaccacc gccagatcgc cgacttcttt tttcagcaat
    10201 gcgcgggcca tcggggaatc gatagagatg taatctttac ggccaaaaat ttcatcgtag
    10261 ccgacaatac ggaaacggtg agtcacgcca tcgtcgtttt caatctccac ccacgcgcca
    10321 aaaaagactt tgccttcctg ctgaggggag taatcgacga ttttgagatt ttccaggcat
    10381 ttagtgagat agcgcacgcg acggtcgatt tcacgcagac gctttttatt atactgatag
    10441 tcagcatttt cgctgcggtc gcccagactt gcggcccagg tcaccttttt tgtgacctcc
    10501 gggcgttctt cacgccagag ataattaagc tcttgtttga gtttttcata cccttcccgg
    10561 gtaaccaggg gcgttttcat ctcgttgatt ccctttgtct gtttgataat gcgcacattg
    10621 ggtataacgt gatcatatca acagaatcaa taatgtttcg ccgaataaat tgtatactta
    10681 agctgctgtt taatatgctt tgtaacaatt taggctgaaa ttcataccag atttagctgg
    10741 tgacgaacgt gagctttttt aagaatacac gcttacaaat tgttgcgaac ctttgggagt
    10801 acaaacaatg caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgcgc
    10861 gctgctggag cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga
    10921 acagatggat cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttgatgtt
    10981 acctggtgaa gatggcttgt cgatttgccg acgtcttcgt agccagagca acccgatgcc
    11041 gatcattatg gtgacggcga aaggggaaga agtggaccgt atcgttggcc tggagattgg
    11101 cgctgacgat tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtatccgtgc
    11161 ggtgctgcgt cgacaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat
    11221 tgctttcggt aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagacgagcc
    11281 gatgccgctc accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga
    11341 gccgctctcc cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga
    11401 acgctccatc gacgtgcaga tttcgcgtct gcgccgcatg gtggaagaag atccagcgca
    11461 tccgcgttac attcagaccg tctggggtct gggctacgtc tttgtaccgg acggctctaa
    11521 agcatgaggc gattgcgctt ctcgccacga agttcatttg cccgtacgtt attgctcatc
    11581 gtcaccttgc tgttcgccag cctggtgacg acttatctgg tggtgctgaa cttcgcgatt
    11641 ttgccgagcc tccagcagtt taataaagtc ctcgcgtacg aagtgcgtat gttgatgacc
    11701 gacaaactgc aactggagga cggcacgcag ttggttgtgc ctcccgcttt ccgtcgggag
    11761 atctaccgtg agctggggat ctctctctac tccaacgaag ctgccgaaga ggcaggtctg
    11821 cgttgggcac aacactatga attcttaagc catcagatgg cacagcaact gggcggcccg
    11881 acggaagtgc gcgttgaggt caacaaaagt tcgcctgtcg tctggctgaa aacctggctg
    11941 tcgcccaata tctgggtacg ggtgccgctg accgaaattc atcagggcga tttttccccg
    12001 ctgttccgct atacgttggc gattatgcta ttggcgatag gcggggcgtg gctgtttatt
    12061 cgtatccaga accgaccgtt ggtcgatctc gaacatgctg ctttgcaggt cggtaaaggg
    12121 attattccgc cgccgctgcg tgaatatggc gcgtccgagg tacgttcggt tacccgcgcc
    12181 tttaaccata tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg
    12241 ggggtaagcc atgatctgcg cacgccgctg acgcgtattc gcctggcgac cgagatgatg
    12301 agcgagcagg atggctacct ggcagaatcg atcaataaag atatcgagga gtgcaatgcc
    12361 atcattgagc agtttatcga ctacctgcgc accggacagg agatgccgat ggaaatggcg
    12421 gatctcaacg cagtactcgg tgaggttatt gctgccgaaa gtggctatga gcgggaaatt
    12481 gaaaccgcgc tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc
    12541 gcggtggcaa atatggtggt caacgccgcc cgttacggca atggctggat caaagtcagc
    12601 agcgggacgg agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg
    12661 ccggaacaac gtaagcacct gttccagccg tttgttcgcg gcgacagtgc gcgcaccatt
    12721 agcggcacgg gattaggact ggcgattgtg cagcgtatcg tggataacca taacgggatg
    12781 ctggagcttg gcaccagcga gcggggcggg ctgtccattc gcgcctggct gccagtgccg
    12841 gtaacgcggg cgcagggcac gacaaaagaa gggtaaaaaa acgggaggcg aaggtgcctc
    12901 ccgttttgct ttctctaaga tactggatag atattctcca gcttcaaatc attacagctt
    12961 cggaccagcc gctaccagcg cggcacccgc tggggtgtcg gtgtatttat cgaagttgtc
    13021 gataaacagt ttcgccaggg tttcggcttt ttcctgccac tgttccgggg aagcataggt
    13081 gttacgcgga tcgagaatct tcgtgtctac gcccggcagt tcggttggga tcgccaggtt
    13141 aaacatcggc agagtgaaag tttctgcgtt atccagcgaa ccgttgagga tggcgtcgat
    13201 aatggcgcgg gtatctttaa tcgagatacg tttgccagtg ccgttccagc cagtgttaac
    13261 cagataagcc tgcgcgcccg ccgcctgcat acgtttcacc agcacttctg cgtactgagt
    13321 cgggtgcagc gacaggaatg ccgcgccgaa gcaagcggag aaggttggcg tcggttcggt
    13381 gatgccacgc tcagtaccgg ccagtttggc ggtgaagcca gagaggaagt ggtactgggt
    13441 ttgatcggca gtcagacgag aaaccggtgg cagcacacca aaggcgtctg cggtcaggaa
    13501 gataacttta gttgcgtggc ccgctttgga aaccggctta acgatgttat cgatgtgata
    13561 gatcggataa gaaacgcggg tgttctcggt ttttgaacca tcatcaaagt cgatagtgcc
    13621 atcttcacgc acggtgacgt tttccagcag cgcatcgcga cggatagcgt tgtagatttc
    13681 aggttccgct tctttcgaca gcttgatagt ttttgcgtag cagccgcctt cgaagttaaa
    13741 cacgccgtca tcgtcccagc cgtgttcgtc atcgccaatc aggcgacgtt tcgggtcggt
    13801 ggaaagggtg gttttaccgg tgccggaaag gccgaagaac accgcaacat cgcctttctc
    13861 gccaacgttg gcggagcagt gcatagaagc gatacctttc agcggcagca ggtagttcat
    13921 catcgagaac atccctttct tcatttcgcc gccgtaccag gtgccgccaa tcagctgcat
    13981 gcgctcggtc aggttaaacg ccacgaagtt ttcggagttc agaccctgtt ctttccactg
    14041 cgggttagtg cacttcgcgc cgttcataac gataaagtct ggtttgaaac ctgccagctc
    14101 ttcatcgctc gggcgaataa acatgttttt gacaaaatgc gcctgccagg ccacttcggt
    14161 gatgaaacgg acggaaagac gagtatccgg gttcgcacca cagaaagcgt cgacaacgaa
    14221 cagacgtttg ccggaaagct gtttggtcac caggcctttc agatgttgcc aggtttccgg
    14281 agagagaggt ttgttatcgt tcttaccttt gcctttgtct gcccaccaga aagtatcgcg
    14341 agtggtatcg tcacggacga tatacttatc ttttggtgaa cgaccggtga agatcccggt
    14401 atcgacggca acggcaccca gattagttaa caccccgcgc tcataacctg tcaggctcgg
    14461 atcgagctct tcctgataca gcaggtcgta gcttgggttg taaacgatat catgtacgtc
    14521 actgatacca taagcctcga gttcttgcgg ggtcaaacca ttgttaacgc gcatttcact
    14581 gctccttagc caatatgtat tgcctgaata gtaaagtctt tttgggggtg ttaaccgcga
    14641 caaggctcat agatttacgt atctggagaa attcatattt gacaaaaaac tccgtgattc
    14701 ctgtcacgaa acggttgcta ttatcgcaga aatagcattt taggtgtgga aaatgttctc
    14761 aaaaggttaa atcttggttt atatggcggg aatattgtga gtggaatcgc attcatgaat
    14821 gaaattgatg caaatttaat cggtaaagta tcgattcacc acgctaactt tttttgatta
    14881 ttttctgaaa aaagcaaaat actactgccc ggatgcgata attaacccac cttttgattc
    14941 tggataacca acatggataa cgttgaactt tcaccggcaa cacgctgggg gatgattgct
    15001 accggattac ttcagggtct ggtttgctac ctgctgatcg cctggcttgc cggaaaaaat
    15061 cacagttgga ttgtttatgg cgtgcctgcg accgtcgcgt tttcatccgt tttactcttc
    15121 tccgtgatct cttttaaaca gaagcgcctt tggggatggc tggcgctagt gtttattgcc
    15181 acgctgggga tgagcggctg gctgaagtgg caaactgatg gcatgaaccc ctggagagct
    15241 gaaaaggcgc tttgggattt tggttgctat ctgctgctga tggcaatgtt gttgctaccg
    15301 tggatacaac aaagccttcg catccgcaat gacagtagcc gctatcgcta tttttaccag
    15361 tcagtatggc ataacgtact tatattactg gtgatttttc tcgccaatgg cttgacgtgg
    15421 ctagtgcttt tactgtggag tgagttgttt aaacttgttg gcatcacgtt tttcaatacg
    15481 ctcttttttg caaccgactg gtttatttat ctcacgttag gtctggttac cgcgctggcg
    15541 gtgatcctcg cgcgaacaca gtcacgttta atcgactcta ttcaaaagtt gttcacgtta
    15601 atcgccacgg ggttgctgcc gttagtatca ttgctaaccc tgatgtttat catcaccctg
    15661 ccgtttacgg gcctgagcgc gatttctcgc cacatctccg ccgccgggtt gctgttgacg
    15721 ctggcctttt tgcaattgat cttaatggct attgtccgcg atccgcaaaa agcgtcactt
    15781 ccctggacag ggccgttgcg ttgcctgatt aaaaccgctt tgctggctgc tccgctgtat
    15841 gtgttcgtcg ccgcctgggc gttatggctg cgggtcgctc agtacggctg gactgtcgac
    15901 cgcttgcagg gcgtgctggc ggtgctggtg ttactggtgt ggtcgctggg gtattttgtc
    15961 agcatcgtct ggcgtaaagg gcaaaatccc gttgttcttc agggcaaagt gaaccttgcg
    16021 gtttcgttat tggtgttggt gatactggtg cttcttaatt cgccggtgct ggacagtatg
    16081 cgcattagcg tgaacagtca tatggcgcgt tatcagagcg gcaaaaacac gtcagaccag
    16141 gtaactatct acatgctcga gcagagcggt cgctatggac gtgctgcgct tgagtcgctg
    16201 aaaagcgatg ccgggtttat gaaagacccg aaacgcgcgc gggatctgct gatggcgtta
    16261 gatggagagc aacatcttca ggaacaggta tcggaaaaag tattagccga aaatgtgtta
    16321 attgcccctg gttctgttaa accggatgcg acattctggt cggccttaat ccaggatcgc
    16381 tataacgtga tgacctgcat tgaaaaagac gcctgcgtcc tggtcgagca agatctgaat
    16441 agtgatggca gggcagagcg gatcctcttt gcctttgatg atgaaagata cattgtctat
    16501 ggctttgatc ctgacaagaa agaatggcaa gagcttacga tgagtttact tccgcgtgat
    16561 ataacgaaag aaaaattact cacagctgcg aaggatggaa aactgggaac gaagcctaaa
    16621 gcgtggcgcg atcttgtagt ggatggtgaa aggctggatg tgaatctgaa tgagtgacaa
    16681 agcgcatcag gtgtattagt cgtaaaatcc ggcagagtcc tctctgccgg acatactcat
    16741 taatgaactt gcggatctgc cggagacgcg ttgttgcgga tttcagcaat atccatcgca
    16801 ttgaacagat agtggttacc gcagtaatca caatgcatgt caatttcgcc atcttccgcc
    16861 aggatgctat cgacttcttc atcaggcagc gttttcagcg cgtcggcgca acgttcacgc
    16921 gagcaggtgc atttgaactt tacatcctgc ggatcgtaaa ccgtcacctc ttcttcgtga
    16981 tacaaacgcc acaacacttc gtttgccggt aaggtcagca gttcttcggt tttgatggtt
    17041 tcggttagcg tcgccaggtg gtcaaagtcg tcctgctggg cattttgcgc aggcattacc
    17101 tgcaacaaca taccgcctgc agccggttta ccgtctacgt cgccggtgcg aataaacagg
    17161 cgcgtcggca gctgttcaga acgcataaag taatcttcca ggcaggccgc cagggtatca
    17221 ccttccagac caaccacgcc ctgatagcgt tcgccttcgc tcggggtaat ggtgatcacc
    17281 acgtaaccat tgccgaccag cgttttcagg tcggcatttt ccggaatttc gccctgtacg
    17341 cgagccacac cgcgcatctg ctggttgttg tttccgttga tgaccgccag actcatcggg
    17401 ccgtcgcctt gcagttgtac ggtgatatca ccatcaaact tcaacgtagc ggttaacagg
    17461 ctggtcgcaa ccagcagttc cgccagcacg tttttaacgg gctgcggata atcgtgattc
    17521 tcaaggatct gttgcagggt ttccgaaacg gttaccagtt cgccgcgcac ggcaaagttt
    17581 tcaaacagat agcgatgtaa ttggtcatgt tgcggcataa tcatctctct tgcaggtgac
    17641 agttattcac tgtcgccgtg tttaaatcgt aacaggtcgc ggcgctcttt tttgtccggg
    17701 cgtcggtccg ggtgcggcat ggttaaggca ttaagtttac gtgccagcgc cattttttcg
    17761 cgtttctcta cactttccgc agtctcttca tacagcaagg ctgcctcgct ggcggggcga
    17821 cgctgttcag taatcgcctt tacaatcacc gtgcgttcgt catttccctg gcgcagagtg
    17881 agcgtggcat tcagctcgac gattttgctc ggcttgctgc gctgcccgtt gtaatgcacc
    17941 ttaccgcctt caatcatttc acgggccagc gcgcgggttt tataaaaacg ggcagcccat
    18001 agccatttat ccagtcgaac ctcaacagca ggtttttctt tcatggcgtc tccttcacat
    18061 tagcgagggg atcaggcggc ggtagtcatt cagtgacgga tggcgttgat actgtttctc
    18121 ggcaatcccg gagtcaggat tagtcacgcc gaggcagtaa cgaataccaa attgcgcggc
    18181 agcatcgaga atcgcttcgc tgtcatcaat aaacagcgtt ctttcagctt tcagacccgt
    18241 agcttcggcc accgcatgcc ataaccgctg atcctctttc ggataaccaa atgtgtgggt
    18301 ggaaagtaat aaatcaaggt gtgcgtccag accggtatgc tcaagtttta ccgccaggtt
    18361 gtgcggatgc gcattggtga gcaaaattcg ctgcttaccg ctggctttca gtgcctcaag
    18421 aaacggaatg gtatcttcac gcagtacggc acgcggtccc atctcggtgg tcatcgcaca
    18481 gatatccaga cccagttgct cactccagta atcaagacag taccagttta gcgtatgctg
    18541 tacgtcgtga tattgctggc gcatatattc catcgcttcc tgtggcgtaa ccccgttttt
    18601 cgcgccccat gtttcaggca ccagcttttg ccagaaatag ttatcgaagg cgaggtcgag
    18661 caacgtgccg tccatatcca gcagaacggt atctacgtcc tgccaggcaa tgttgatatg
    18721 catgagggaa atctccagag tgaagcaatt tgcgcgacag ggtagcataa cctgccgcgc
    18781 aaacgtgtta ttcgataagg ctttctgaag gggtgatcag ttgcgggttc aggcagcttt
    18841 cataataggc ctgaatcttc atcatgcgag tgcgatgacg ctggtaacgc cgccaggcct
    18901 gtacgccatt ataaatggcg ctacccagca tcgtcagtag cagcagcgtg gtgctgagat
    18961 agcgccacag gccggaacga tccgggatcg gatgaaggcc tatatgctgc gtaccattgg
    19021 cgtcagtgaa gatttttgtg acgataccct cggcgttaaa cggcgtatgc atcagcattt
    19081 gtgccagttt ctggaaagcg ttccactgtt cttgcggcgg atagtcgtaa agtgatgccg
    19141 aaggccaggg ctgatcaaca aaatcgctgc cttcgtcgct gacaatcagg aatccgccgg
    19201 gggccggact gtttagtgat tgtgccgctc gcgccgtttc atgcgtgata aacggcgcgg
    19261 tggaggttgc caccaggtta tccagcgatt ccgcactcac cgggcgtaat aacacattca
    19321 cgccatcgag cttcccggcg ttggcgcgtt ttaccagcgc gtcccagtct ttactgttgc
    19381 cgagattgac cagcgcattt ttcagtcgca cacagtcatc tttggcagaa cacagatccg
    19441 ctgtcttcag tacaatgtcg ccaaaatcat caagcaatac catgccggat ttttgaattg
    19501 ctgaacgtaa tgaggcactg acgcgagatt catcttccgg tttagggtgc agctggcgat
    19561 taactgcttc agtcaatgcc gtcgctttgt tgaccagttc agattctggt aatggcaatg
    19621 agcgggcgtc attccagatg atctgcgagc agtcaaacgg taaaaaaggt gaattggttt
    19681 tcgcgctcca ggttccggaa gtgcgaatat tacacattcc cgtaccgcta atacgcaatg
    19741 tatcgcctac ccgcacgcca gcgtcagcca gttgttttac gctggtggct tcaatggtct
    19801 gcgcgccttt catccatgag agagtgaatt tcagcggcat atccagcggg atccagaata
    19861 acagcataaa cagcaccagc agcgagcccg cagcgataat cgtactgcgc agccagtgct
    19921 gtaacggaaa gttttttact tcatcatgca gcgaaagata tcgcccctga cgcactacgt
    19981 ggcggtcgag atagatatcg atatcggttt gttgaccgag atcctgagca atgtatggct
    20041 gccagtgtgc gggatagacc aggtcgataa taccgagtga aatattgttg atctgttcct
    20101 gatcgttttc gccaaacaat ccccaacgac ggggtgtacc gcgcagacaa tgaatttccc
    20161 tcagggagga ttttgccggt ggcgcgaata gcccccacag accagcgccc agcagcagta
    20221 acgcgccgcc tgccagccac ggaacaaata catccggggt aatcaggcag aaaaagaaca
    20281 tcagaaagga ggcgacgatc agcaacgctt cgcgcagccc gcgcggacga ctcagcgcat
    20341 actcttcgtg cgtttctttg cgaatattga gtagctcgat ttgctcactc tcttccccgc
    20401 gaatcgacgc ttgcgtggac ggaacaggtt gcaaggcata gccgcgcgtt tcctgcatgt
    20461 actcctgcag cgtatgaccg ttgagtgaaa taaccagcgg cagcgaatcg gtatgaatca
    20521 gttcaacggt attctcatcg ttgatgtact gttcccagaa agggggcagg tggacttcta
    20581 ccgaatcgag gtagtaacgc catttattcg gatcgtcggt agagatgccg taacgcgtga
    20641 tagcgtgtgt cagcatcatg acgttgttgc tttcggcatt cagcgccaga gagatcggtg
    20701 ccgcgctggc tcccgttggg ccaggcacct gtaatacctg cgtcaggctc tcaagataat
    20761 tttcaacggc gctacgttct tcaggtgtga gtttacgcgt ttgcgcatcc gcgaaggcgt
    20821 tggtccaggg cagctgacgc cgtctggatc gcacttttat cagccatccc gcaagtagtg
    20881 agcaggccag caaagcagct aaaaaaatca caatggtgct catgctttcc ccatcttact
    20941 tagtctgtca ggcgtggtca gtcgcaccgg acacaataca tgattctgcg gttggctatc
    21001 ggcaagcgtg gagtttaact tgagcatcat taagcacatc acagtaaccg ctgatcatag
    21061 caaaccctct gaaggcggaa tatcagcaaa ttcctggagt tatttcaacc tattgacttt
    21121 taatttgatt tgaggacgat tatttattaa gttacataaa tgtaaataac atgcaattca
    21181 cattgccgaa agcgtgtccg atatcgcaca ataaccgctg atttcacagc atagatccca
    21241 cgatgagcaa atcattacaa aaacccacca ttctgaatgt tgaaactgta gcccgttccc
    21301 gactgtttac cgtcgagagc gtggatctgg agttcagcaa tggcgtgcgg cgtgtttatg
    21361 aacgaatgcg tccaaccaac cgggaagcag tgatgattgt gccgattgtg gacgatcacc
    21421 tgatcctgat ccgcgaatac gcagtgggaa ctgaatccta cgaattaggt ttttcgaaag
    21481 gattaattga tccgggtgaa agcgtctacg aagccgctaa ccgcgagcta aaagaagagg
    21541 ttggatttgg tgcgaacgat ctgacttttt tgaagaagct cagcatggca ccgtcttact
    21601 tttccagcaa aatgaatatc gtggtagcgc aagatctcta cccggaatcg ctggtaggcg
    21661 atgagccaga gccgctaccg caggtgcgct ggccgctggc gcatatgatg gatttgctgg
    21721 aagaccctga cttcaacgaa gcgcgtaatg tcagcgcgct gttcctcgtg cgcgaatggt
    21781 tgaaagggca ggggcgagtg taaatgcgtg aagtttgcct gatgcacaat gtttatcagg
    21841 catacggagt tcctgcaatt tattgagttt tcacgatttt gttggcctga taacgcgttc
    21901 acccgcatcc ggcataaaca aatgcacttt gtcagccaac tgaaaaggcg ccgaagcgcc
    21961 tttttaatca gaacaattcc tgtgcctcac cattatcgat aatggtcgtt cccacctcgt
    22021 gcaccgcctg ttgtgtcggc tgcgtacctt cgatgaaata ctcttcgcgg ctgttgccac
    22081 cattagctaa ctgcccggtg ctgcgatcga tattcaccgt cacaataccc ggaggcggcg
    22141 tcagcggctg ctccggcaca ccttcaagaa cggctttcat ataagcgtcc catgcaggct
    22201 gggcactctt ggcaccgcct tcgtaacctg agatctgatc tttaatcgct ccggaagccg
    22261 ttgtatgacc gagattacga cggtgatcat caaagccaat ccagaccgag gtcacaacgc
    22321 ccggaccgta acccgagaac cacgcatctt tcgaactgtt agtggtaccg gttttcccgc
    22381 cgatatcgcg acgctgcaaa tcacgacctg cacgccagcc ggtaccctgc cagcctggtt
    22441 caccaaagat attggtgttc aaagcactct taatcaggaa tgccagcgga gtgttgatga
    22501 cgtgcggtgc gtactcctgc gcgccagtct tcgccactaa cgcctgattt gcctgctcca
    22561 gctgcggcat tggtacagaa acattctgct gctcgcggga gatagcgaca tcttcaacat
    22621 cgttattttc cagcacgttc gatttctgcg tatcaccgta aatcaccgga atatcgcatt
    22681 ccgggcaggc tactttcggt ttcgcttcga aaatcacgcc gccctgatcg ttttcaattt
    22741 tgctgataaa ccacgggtcc accaggaagc caccgttcgc catgaccgcg tagccgcgcg
    22801 ccacctgcat tggggtgaag gacgctgaac ccagcgccag cgattcggtg tggacaatgt
    22861 tttgcgccgg gaagccgaag cgttgcagat attctgcagc gtagtcgacg cccatcgccc
    22921 gcattgcgcg taccatcacc acgtttttcg actgacccag cccctgacgt aagcgaattg
    22981 gaccagcata ctgcggtggt gagttcttcg gctgccagtc agaaccggca cctgcatccc
    23041 agcgagaaat tggcacatcg ttcaacatac ttgccagcgt cagaccttta tccatcgccg
    23101 cggtgtagag gaacggtttg atgttggaac ccacctgacg cagtgcctgg gtggcgcggt
    23161 taaacttgct ctgattgaaa tcaaagccac cgaccagcgc cataacggca ccgttttgcg
    23221 gattgatcga caccagcgcc gagttcactt ccggcacttg tgccagccac catgcatcgc
    23281 caacctgacg aacccagatt tgctgacccg tttgcagaac atcggtcact ttacgcggcg
    23341 tcggtccttg ctgagtatcc gaacggtaag gacgcgccca gcgaacgcct tccatactca
    23401 atgcgacggt cgacccgtcc gccagcatcg ccgtcgcttg ctgaggattg gcgctggtga
    23461 ctgcggcagg cagcagcgga ccataggttg gcagcgcctt cagcgtatcg gtaatcttgt
    23521 tgttatccca cgccgactcg cccactttcc acagcacatt tgccgggccg cgatagccgt
    23581 ggcgcatgtc gtagtccagc acgttattac gtaccgcctg ctgtgcggcc tgctgcactt
    23641 tgcgggtgat ggtggtgtaa atgcgataac cgtcttcata ggcactttcg ccataacggt
    23701 tatacatctc ctggcgcacc atttcgctca ggtacggcgc agagaaagca atctccggcg
    23761 cgtgatagtt agcgttaatc gcctcagtgc gtgtctgatc gaactgttgt tgggtgatat
    23821 acccttcatc cagcatccgc gacagtacga cgttacgccg cgcgacggca cgatccattg
    23881 agtagagcgg attaaaggtg gaaggcgctt tcggcagccc ggcgatcaca gccatttcgt
    23941 tcagcgtcag ttggtcgacc gttttaccga aatagacttg tgccgcagca ccgacaccat
    24001 aggcgcggta accgaggtaa atcttgttca gataaagctc gaggatctcg tctttcgtca
    24061 gcaactgttc aatgcgaatc gcgaggaaga cttccttaat cttacgcatc agcgtgcgtt
    24121 ctggactgag gaagaaattt ctcgccagct gctgggtaat ggtactcgcc ccttgtgacg
    24181 cgtggccgga gaacagcgcc acacttgccg cacggaagat ccccaccggg tcaacgccgt
    24241 gatgctcgta gaagcggctg tcttccgtcg cgataaaggc tttcaccatc tccggtggga
    24301 tttgatccaa cgtaaccgga atacgacgtt tctcaccgta ttgagcaatc agctcgccat
    24361 cggcgctgta aatctgcatc ggaatttgca ggcgaacatc ttttaatgtc gccacatccg
    24421 gcagttgtgg ctcgatgtag cggtataggc cataaatcga gcctgctccc agcaaaatgc
    24481 aacagactgc aaggatcaaa aaatacttta cgaacttcac tggaaatttc ccatttagtt
    24541 tcatttgggc agtttataaa caaacgcgcg gtagtataaa ggcaagccag acgcattgat
    24601 atacccgtca gagtgacggg tgataaggag atcatcacaa tggcatttaa gatctggcaa
    24661 attggtttgc atttacaaca gcaagaagcg gtagcggttg cgatcgtacg gggcgcaaaa
    24721 gaatgctttt tgcaacgctg gtggcggttg ccgctggaga acgacattat caaagatggg
    24781 cggattgttg atgcgcagca gctggctaaa acgttgttac cttggagtcg cgaactgccg
    24841 cagcgtcatc acattatgtt ggcgtttccc gccagtcgca cattacagcg gtcatttccg
    24901 cgcccgtcga tgtcccttgg tgagcgggag caaacggcct ggctgtcagg gacgatggcc
    24961 cgcgagctgg atatggatcc ggactccctg cgcttcgatt atagcgaaga ctcactcagc
    25021 cccgcttata acgtgactgc cgcgcaaagc aaagagctgg caacgctgct tactctggca
    25081 gaaaggttgc gtgttcatgt tagtgcgatc accccggatg ccagtgcatt acagcgattc
    25141 ctgccttttt taccttctca tcagcaatgt ctggcctggc gtgataacga acagtggctg
    25201 tgggcgacac gctatcgctg ggggcgcaaa ctggcggtgg ggatgactag cgcgaaggag
    25261 ctggcggcag cgttatccgt tgatcccgaa agcgtcgcga tatgtggcga agggggattt
    25321 gatccctggg aggccgtttc tgttcgtcag ccgccgctac cgccgtcagg tggagacttt
    25381 gccatcgcgc tggggctggc gcttgggaag gcgtactgat gaacccgcca attaattttt
    25441 tgccctggcg acagcaacgc cggaccgctt ttctgcgttt ctggttgctg atgttcgttg
    25501 cgcctctgct gctggccgtc gggataacgc taatactgcg tctgacaggc agcgccgaag
    25561 cgcgcataga cgcagttttg cttcaggcgg aacaacaact cgcccgcagc ttacagataa
    25621 cgaagccacg tttgctggag caacagcagt tacgcgaaca gcgttcgcaa cggcagcgcc
    25681 agcgacaatt tacccgcgac tggcaatctg cgctggaagc actggcggcg cttttacccg
    25741 agcacgcctg gctgacaacg ataagctggc agcagggaac gctggagatc aaggggctga
    25801 caacaagcat taccgcgtta aacgcactag aaacgtcact ccgccaggat gcgtcttttc
    25861 atctcaatca gcggggagcc acgcagcagg atgcgcaggg acgctggcaa tttgagtatc
    25921 agttaacaag gaaggttagc gatgaacatg ttctttgact ggtggttcgc cacatcaccc
    25981 cgcctccgcc agctttgctg ggcattctgg ttgctgatgt tagttacgct catttttcta
    26041 tcatcgacac accatgaaga gcgcgacgca ttaattcgac tacgggcaag tcatcaccag
    26101 cagtgggccg cactgtatcg cctggtagac accactccct tcagcgagga aaaaacgctg
    26161 cccttttcgc ctctggattt tcagttatcc ggcgcgcaac tggtttcctg gcatccatcc
    26221 gcgcagggag gtgagttggc gttgaaaacg ctgtgggaag cagtgccgtc ggcatttaca
    26281 cggctggcag agcgcaacgt cagcgtgagc cgtttttcgt taagcgtgga aggtgatgat
    26341 cttttgttca cgctacaact ggagacaccg catgagggtt aaacgctggt tgttggcagg
    26401 tattgcattg tgccttttaa ccggtatgcg tgaccctttt aaaccgccgg aagatctatg
    26461 ccggattagc gaacttagcc agtggcgcta tcaggggatg gtagggcgag gcgagcacat
    26521 catcggtgta ataaaagacg ggcaaaagaa atggcgacgg gtgcagcaaa acgatgtgct
    26581 ggaaaacggc tggacaattt tacagctgac gccagacgca ctaacgctgg gaaccgggac
    26641 aaactgcgaa ccgccacaat ggttgtggca acggcaagga gatacaaatg aagcaatgga
    26701 tagccgcact actgttgatg ctgatacccg gcgtacaggc ggcaaagccg caaaaagtga
    26761 cgctgatggt ggatgacgtt ccggtagctc aggtgttgca ggcgctggct gaacaggaga
    26821 agttgaacct ggtcgtgtcg ccagacgtca gcggtacggt gtcgttacat ctaacagatg
    26881 ttccctggaa gcaggcacta caaactgtag tgaaaagcgc cggactgata acgcggcagg
    26941 aaggcaacat tctctcagtg cattccattg cctggcagaa taacaatatc gcccgccagg
    27001 aggcggagca ggcgcgggcg caggcaaatc tgccgctgga aaatcgcagt ataaccctgc
    27061 aatacgccga cgcgggagaa ctggcgaaag cgggggagaa gctactgagt gccaaaggga
    27121 gtatgaccgt cgataaacgc accaatcgtc ttttgctacg agataacaaa acggcgttaa
    27181 gcgcgcttga acagtgggta gcgcaaatgg atctgccggt cgggcaggtt gagctgtcgg
    27241 cgcatattgt caccattaat gaaaaaagtt tgcgtgagtt aggcgtgaaa tggacgctgg
    27301 ccgatgcgca acacgctggt ggcgttgggc aagtcaccac gcttggtagc gacctctccg
    27361 tagcgacggc gacaacgcat gtcggtttta acattgggcg catcaacgga cgcttgctgg
    27421 atcttgagct ttccgcgctc gaacaaaaac agcagctgga tattatcgcc agtccgcgtc
    27481 tgctggcctc acatcttcag cctgccagca ttaaacaggg gagcgaaatt ccatatcagg
    27541 tttccagcgg ggaaagtggc gcgacgtcgg tggaatttaa agaggccgtc ctggggatgg
    27601 aggtcacgcc cacggtgtta caaaaaggtc gcatccggct gaaattacac atcagccaga
    27661 acgttccggg gcaggtgcta cagcaggccg atggcgaagt gctggcgatt gataagcagg
    27721 agatcgaaac gcaggtcgag gtcaaaagcg gagaaacgtt ggcgctgggc ggcattttta
    27781 cccgtaaaaa taaatcgggt caggatagcg taccgttgct tggcgacatt ccctggttcg
    27841 ggcaattatt tcgtcatgac ggaaaagaag atgaacgacg cgagttagtg gtgtttatca
    27901 cgccacgact ggtttccagt gagtaaacag ccgtaaaagc ggtaatgttt ttatgctgaa
    27961 cgtgtttcat ctatttgacg cgcgcaggta tttagcatac aaggagtacc gatttgagag
    28021 ttggtgctct tcgctgcctg cgttccatga tgatgattta tcattcaggc ggcattttgc
    28081 tgtctttttt acgctaatct tacccggtga tttatcgcca gagcggtggt agcaaggcag
    28141 cgcgcttgca gcgaccagat atgcagaggg atgggtgatt tattcagttg ccaaacccgc
    28201 tggagtattg agataatttt cagtctgact ctcgcaatat cttatgaggt ttcagttcat
    28261 gtcctgcggc gctctctgag cgaagcgggt ttatcattaa cgaatagtct tagtagtacc
    28321 gaaaaaatgg cagagaaacg caatatcttt ctggttgggc ctatgggtgc cggaaaaagc
    28381 actattgggc gccagttagc tcaacaactc aatatggaat tttacgattc cgatcaagag
    28441 attgagaaac gaaccggagc tgatgtgggc tgggttttcg atttagaagg cgaagaaggc
    28501 ttccgcgatc gcgaagaaaa ggtcatcaat gagttgaccg agaaacaggg tattgtgctg
    28561 gctactggcg gcggctctgt gaaatcccgt gaaacgcgta accgtctttc cgctcgtggc
    28621 gttgtcgttt atcttgaaac gaccatcgaa aagcaacttg cacgcacgca gcgtgataaa
    28681 aaacgcccgt tgctgcacgt tgaaacaccg ccgcgtgaag ttctggaagc gttggccaat
    28741 gaacgcaatc cgctgtatga agagattgcc gacgtgacca ttcgtactga tgatcaaagc
    28801 gctaaagtgg ttgcaaacca gattattcac atgctggaaa gcaactaatt ctggctttat
    28861 atacactcgt ctgcgggtac agtaattaag gtggatgtcg cgttatggag aggattgtcg
    28921 ttactctcgg ggaacgtagt tacccaatta ccatcgcatc tggtttgttt aatgaaccag
    28981 cttcattctt accgctgaaa tcgggcgagc aggtcatgtt ggtcaccaac gaaaccctgg
    29041 ctcctctgta tctcgataag gtccgcggcg tacttgaaca ggcgggtgtt aacgtcgata
    29101 gcgttatcct ccctgacggc gagcagtata aaagcctggc tgtactcgat accgtcttta
    29161 cggcgttgtt acaaaagccg catggtcgcg atactacgct ggtggcgctt ggcggcggcg
    29221 tagtgggcga tctgaccggc ttcgcggcgg cgagttatca gcgcggtgtt cgtttcattc
    29281 aagtcccgac gacgttactg tcgcaggtcg attcctccgt tggcggcaaa actgcggtca
    29341 accatcccct cggtaaaaac atgattggcg cgttctacca gcctgcttca gtggtggtgg
    29401 atctcgactg tctgaaaacg cttcccccgc gtgagttagc gtcggggctg gcagaagtca
    29461 tcaaatacgg cattattctt gacggtgcgt tttttaactg gctggaagag aatctggatg
    29521 cgttgttgcg tctggacggt ccggcaatgg cgtactgtat tcgccgttgt tgtgaactga
    29581 aggcagaagt tgtcgccgcc gacgagcgcg aaaccgggtt acgtgcttta ctgaatctgg
    29641 gacacacctt tggtcatgcc attgaagctg aaatggggta tggcaattgg ttacatggtg
    29701 aagcggtcgc tgcgggtatg gtgatggcgg cgcggacgtc ggaacgtctc gggcagttta
    29761 gttctgccga aacgcagcgt attataaccc tgctcaagcg ggctgggtta ccggtcaatg
    29821 ggccgcgcga aatgtccgcg caggcgtatt taccgcatat gctgcgtgac aagaaagtcc
    29881 ttgcgggaga gatacgctta attcttccgt tggcaattgg taagagtgaa gttcgcagcg
    29941 gcgtttcgca cgagcttgtt cttaacgcca ttgccgattg tcaatcagcg taacaacaag
    30001 aaaggtcagg ccgcttatca agcggtctat tagcttcagg ttaattgcaa cgtggtaagc
    30061 attaaccttt tagtggggtg ttaaatggat gaattcaaac cagaagacga gctgaaaccc
    30121 gatcccagcg atcgtcgtac tggtcgttct cgtcaatctt ctgaacgttc tgagcgtact
    30181 gaacgtggcg aaccgcagat caattttgat gatattgaac ttgatgacac tgacgatcgc
    30241 cgtccgactc gtgcgcaaaa agagcgcaat gaggaaccgg aaatcgaaga agaaattgac
    30301 gaatccgaag atgaaaccgt ggatgaagag cgcgtagagc gtcgtccgcg taagcgcaaa
    30361 aaagcagcca gtaaacccgc ttctcgtcag tatatgatga tgggcgtcgg cattctggtt
    30421 ctactgctgt tgatcatcgg tatcggttct gcgctaaaag ccccctcgac cacttccagc
    30481 gatcaaaccg cgtctggcga gaagagtatt gatcttgctg gcaatgcgac cgatcaggcg
    30541 aatggggtgc agccagcgcc gggaaccacg tctgcggaaa atactcagca ggatgtttct
    30601 ctgccgccga tctcttctac gccgactcaa gggcaaaccc cggcggcaac ggatggtcaa
    30661 caacgtgttg aagtgcaggg tgacctgaac aatgcgctga cccagccaca aaatcagcaa
    30721 ccgctgaaca atgtggcggt caattccacg ttgccgactg aacccgcgac tgtcgcgcct
    30781 gttcgcaatg gcaatgcatc gcgtgacacg gcgaaaacgc aaaccgctga acgtccggcc
    30841 actacacgcc cagctcgtca gcaggcggtg attgaaccga aaaaaccgca agcaaccgtg
    30901 aaaacggagc cgaagccggt agcacagacg ccgaaacgta ctgaaccagt tgctcctgtg
    30961 gcgagcacga aggcaccggc tgcgacttct gcgccagcac caaaagagac ggcgactacg
    31021 gctccagtac agacggcatc cccggcgcaa accacggcaa caccagccgc tggagggaag
    31081 accgcaggta atgttggttc gttgaaatcg gcaccgtcca gccattacac tctgcagctg
    31141 agcagttcct ctaactacga caacctgaac ggttgggcga agaaagagaa tctgaaaaac
    31201 tacgttgtct atgaaacgac gcgtaatggt cagccgtggt atgtcctggt ttctggcgtg
    31261 tacgcttcga aagaagaggc gaaaaaagcg gtatctacat tgccagcaga tgttcaggcc
    31321 aaaaacccgt gggcgaaacc gctgcgtcag gtacaggccg atctgaagta atcaaggtta
    31381 tctcccgcaa tgggttatcg ttgcgggagt tgcctgaagc gctggatgct gtcggagctt
    31441 tctccacagc cggagaaggt gtaattagtt agtcagcatg aagaaaaatc gcgctttttt
    31501 gaagtgggca gggggcaagt atcccctgct tgatgatatt aaacggcatt tgcccaaggg
    31561 cgaatgtctg gttgagcctt ttgtaggtgc cgggtcggtg tttctcaaca ccgacttttc
    31621 tcgttatatc cttgccgata tcaatagcga cctgatcagt ctctataaca ttgtgaagat
    31681 gcgtactgat gagtacgtac aggccgcacg cgagctgttt gttcccgaaa caaattgcgc
    31741 cgaggtctac tatcagttcc gcgaagagtt caacaaaagc caggatccgt tccgtcgggc
    31801 ggtactgttt ttatatttga accgctacgg ttacaacggc ctgtgtcgtt acaatctgcg
    31861 cggtgagttt aacgtgccgt ttggccgcta caaaaaaccc tatttcccgg aagcagagtt
    31921 gtatcacttc gctgaaaaag cgcagaatgc ctttttctat tgtgagtctt acgccgatag
    31981 catggcgcgc gcagatgatg catccgtcgt ctattgcgat ccgccttatg caccgctgtc
    32041 tgcgaccgcc aactttacgg cgtatcacac aaacagtttt acgcttgaac aacaagcgca
    32101 tctggcggag atcgccgaag gtctggttga gcgccatatt ccagtgctga tctccaatca
    32161 cgatacgatg ttaacgcgtg agtggtatca gcgcgcaaaa ttgcatgtcg tcaaagttcg
    32221 acgcagtata agcagcaacg gcggcacacg taaaaaggtg gacgaactgc tggctttgta
    32281 caaaccagga gtcgtttcac ccgcgaaaaa ataattctca aggagaagcg gatgaaacag
    32341 tatttgattg ccccctcaat tctgtcggct gattttgccc gcctgggtga agataccgca
    32401 aaagccctgg caactggcgc tgatgtcgtg cattttgacg tcatggataa ccactatgtt
    32461 cccaatctga cgattgggcc aatggtgctg aaatccttgc gtaactatgg cattaccgcc
    32521 cctatcgacg tacatctgat ggtgaaaccc gtcgatcgca ttgtgcctga tttcgctgcc
    32581 gctggtgcca gcatcattac ctttcatcca gaagcctccg agcatgttga ccgcacgctg
    32641 caactgatta aagaaaatgg ctgtaaagcg ggactggtat ttaacccggc gacaccgctg
    32701 agctatctgg attacgtgat ggataagctg gatgtgatcc tgctgatgtc cgtcaatcct
    32761 ggtttcggcg gtcagtcttt cattcctcaa acactggata aactgcgcga agtacgtcgc
    32821 cgtatcgacg agtctggctt tgacattcgt ctggaagtgg acggtggcgt gaaggtgaac
    32881 aacattggcg aaatcgctgc ggcgggcgcg gatatgttcg tcgccggttc ggcaatcttc
    32941 gaccagccag actacaaaaa agtcattgat gaaatgcgca gtgaactggc aaaggtaagt
    33001 catgaataag tttgaagata ttcgcggcgt cgcttttgat cttgatggta cgctggtcga
    33061 cagtgctccc ggtcttgctg ctgcggtaga tatggcgctg tatgcgctgg agttgcccat
    33121 cgcaggtgaa gaacgcgtta ttacctggat tggtaacggc gcagatgttc tgatggagcg
    33181 cgcattgacc tgggcgcgtc aggaacgtgc gactctgcgt aaaacaatgg gtaaaccgcc
    33241 cgttgatgac gacattccgg cagaagaaca ggtacgtatt ctgcgtaaac tgttcgatcg
    33301 ctactatagc gaggttgccg aagaggggac gtttttgttc ccgcacgttg ccgatacgtt
    33361 gggcgcgttg caggctaaag gcctgccgct aggcctggtc accaacaaac cgacgccgtt
    33421 cgtcgcgccg ctgctcgaag ccttagatat tgccaaatac tttagcgtgg ttatcggcgg
    33481 cgatgatgtg caaaacaaaa aaccgcatcc ggacccgctg ttactggtgg ctgagcggat
    33541 gggaattgcc ccacaacaga tgctgtttgt cggcgactca cgcaatgata ttcaggcggc
    33601 aaaagcggca ggttgcccat cagttggctt aacctacgga tataactacg gcgaggctat
    33661 cgatctcagc cagcctgatg taatttatca gtctataaat gaccttctgc ccgcattagg
    33721 gcttccgcat agcgaaaatc aggaatcgaa aaatgactaa gcccatcgtt tttagtggcg
    33781 cacagccctc aggtgaattg accattggta actacatggg tgcgctgcgt cagtgggtaa
    33841 acatgcagga tgactaccat tgcatttact gtatcgttga ccaacacgcg atcaccgtgc
    33901 gccaggatgc acagaagctg cgtaaagcga cgctggatac gctggccttg tatctggctt
    33961 gtggtatcga tcctgagaaa agcaccattt ttgttcagtc ccacgtacca gaacatgcgc
    34021 agttaggctg ggcactgaac tgctatacct acttcggcga actgagccgc atgacccagt
    34081 ttaaagataa atctgcgcgt tatgccgaga acatcaacgc tggtctgttt gactatccgg
    34141 tgctgatggc tgcggacatc ctgctgtatc aaactaatct ggtaccggtg ggtgaagacc
    34201 agaaacagca cctggaactg agtcgcgata tcgcccagcg tttcaacgcg ctgtatggcg
    34261 agatctttaa ggtgccggag ccgtttattc cgaaatctgg cgcgcgcgta atgtcgctgc
    34321 tggagccgac caagaagatg tccaagtctg acgataaccg caataacgtt atcggcctgc
    34381 tggaagatcc gaaatcggta gtgaagaaaa tcaaacgcgc ggtcactgat tctgacgagc
    34441 cgccggtagt tcgctacgat gtgcagaaca aagcgggcgt ttccaacctg ctggatatcc
    34501 tttctgcggt aacgggccag agcattccag aactggaaaa acagttcgaa ggcaagatgt
    34561 atggtcatct gaaaggtgaa gtggctgatg ccgtttccgg tatgctgact gaattgcagg
    34621 aacgctatca ccgtttccgc aacgatgaag ccttcctgca acaggtgatg aaagacggcg
    34681 cagaaaaagc cagtgtacac gcttcccgta cgctgaaagc ggtgtacgaa gcgattggtt
    34741 ttgtggcgaa gccgtaatat caaaacgctc ggacggtccc ctcgcccctt tggggagagg
    34801 gttagggtga ggggagcagg caagcgccag tgggaattac cctttgctcg cattgtcgaa
    34861 taaatcactt cgtcatcact ctgaaaccgg ggaaacccgg tttttttatc ctcatttgca
    34921 ataatccgaa aaaatgtgaa gcgcctcgcc gtttccacat ctcacagttg ccacttatct
    34981 tcattttaat gaagatataa acattcattt tattgaaaat atattatgcg tcgaacgttt
    35041 atcaaaaaag aaggcgtcgt tatcacgacg ctggcccgtt atttgttggg tgaaaaatgc
    35101 ggtaataggt tgaaaaccat agatgagctg gcaactgaat gccgttcatc cgttggcctg
    35161 acgcaggccg cgttgaaaac gctggaatca agcggagcga tacggattga acgccgtggg
    35221 cgcaatggca gttatctggt cgagatggat aacaaagcat tgctgagtca tgtggatatc
    35281 aacaacgtgg tatgtgcgat gcctttgccc tatacccgct tgtacgaagg tttagcgagc
    35341 ggattgaaag cccagtttga tggcattcct ttttactatg cgcatatgcg cggcgcggat
    35401 attcgcgtgg agtgtttact taacggcgtg tatgacatgg cggtggtttc ccgactggcg
    35461 gcggaaagtt atctcacgca aaaagggttt tgcctcgcgc tggagttggg gccgcacacc
    35521 tacgttggcg agcaccagtt gatttgccgt aaaggcgagt ccgcaaacgt gaagcgcgtg
    35581 gggctggata accgttcggc ggatcagaaa atcatgaccg atgtcttttt tggcgatagt
    35641 gatgtggaac gtgtcgatct ctcttatcac gagagtttac aacgcattgt taaaggcgat
    35701 gtcgatgcgg ttatctggaa cgtggtggcg gaaaacgaac tgaccatgct gggattagag
    35761 gcgacgccgc tcacagacga tccgcgattt ttacaggcca ccgaagccgt ggtcctgacg
    35821 cgagccgatg attacccaat gcaacaactg ctgcgtgccg ttgtagataa acacgccctg
    35881 ctcgcccatc aacaacgcgt ggtgagtggg gaacaggaac cgagttatta aacgaaaggc
    35941 atctgatatg gaaaccagac tcaacctgct ttgcgaggca ggcgttattg ataaggacat
    36001 ctgcaaaggc atgatgcagg tcgtcaacgt actggaaaca gagtgccatc tgccggtgcg
    36061 cagtgagcaa ggaacgatgg cgatgacaca tatggcgagt gcactgatgc gcagtcgccg
    36121 tggtgaagaa atagagccgc tggataacga attgctggca gagctggcgc aatccagcca
    36181 ctggcaagcc gttgtgcaat tgcatcaggt gttgttgaag gaattcgcac tggaagttaa
    36241 cccgtgtgaa gaaggctatt tgctggccaa cctttatgga ttatggatgg ctgctaacga
    36301 agaggtttga agctcccgac aaatccacgt cgcacaacct aaatattcaa aaaaatgaat
    36361 atttaattca aggaacagaa acgaggcaac aagatgtttg tagaagcatt gaaacgccag
    36421 aacccggcgc tgatttccgc cgcactaagt ttgtggcagc agggcaagat cgccccggac
    36481 agctgggtga tcgacgtgga tcaggtactg gaaaacggta agcgactgat tgagacggcg
    36541 cggctttacg gcattgaact gtatctgatg accaagcagt ttggccgtaa cccatggctg
    36601 gcggaaaaat tgctggcatt aggttacagc ggcattgtgg cggtggatta caaagaggcg
    36661 cgagtcatgc gccgcgctgg tttgtcggtg gcgcatcagg ggcatctggt acaaatccct
    36721 tgtcatcagg ttgctgacgc cgttgaacag ggcaccgacg tcatcaccgt gtttactctc
    36781 gacaaagcgc gggaagtttc tgcggcagcg gtgaaggccg gacgtgtgca gtttgtgctg
    36841 ctcaaagttt atagcgacga tgattttctt tatccggggc aggagagcgg ttttgtacag
    36901 cattcgctgc acgaggtggt tgctgaaatt aagaaactgc cggggctgca tttagccgga
    36961 cttacccatt tcccttgcct gctttgggat gaggctgccg gaaaagtttt gccgacaccg
    37021 aatcttcaca cgctgataca ggcacgggat caactggcga aatctggtat tgcacttgag
    37081 caactgaacg cgccttcagc gaccagctgc acttcgctgc cattgctggc ggaatacggt
    37141 gtgactcatg ccgaacccgg tcatgcgctg acgggcacta ttccggcaaa ccagcagggc
    37201 gatcagcctg aacgtatcgc gatgctctgg ttaagtgaaa tctcccatca tttccgtggc
    37261 gacagctact gctacggcgg cggttactac cgtcgtggtc atgcgcaaca tgcgctggtg
    37321 tttacgccag aaaatcaaaa gattactgaa accaatctca aaactgtgga tgacagcagt
    37381 atcgactaca ccctgccgct ggcaggcgag tatccggtga gcagtgcagt ggtgctctgt
    37441 ttccgcacgc agatttttgt cactcgtagc gatgtggtgc tggtgtccgg tattcaccgt
    37501 ggcgaaccag aaatcgtcgg tcgttatgac agtcttggaa actctctggg ggcgtaatgg
    37561 cgcgatttgt ggtgttagtg attgatagct ttggcgtagg ggcaatgaaa gatgtcacgc
    37621 tggtgcgccc gcaagatgcc ggggcgaata cctgtggtca catcctgagc cagttgccgc
    37681 atttgcagct accagcgctg gagaagctgg ggctaatcaa cgcattgggt tatgcaccag
    37741 gtgatatgca accgtcagat tccgcaacct ggggggtggc agagctgcaa catgaaggtg
    37801 gcgatacctt tatggggcat caggaaattt taggcacgcg cccgttaccg ccgctgcgga
    37861 tgccctttcg cgatgtgatt ggccgtgttg agcaggcatt agtttccgct ggctggcagg
    37921 tggagcgccg tggcgatgat ctgcaatttc tgtgggtcaa tcaggcggtt gcgattggcg
    37981 ataatctcga ggcggattta ggccaggtct ataacattac cgccaatctc tctgtgatct
    38041 cttttgacga cgcaatcaaa attggtcgta tcgtgcgtga gcaggtacag gtcggtcggg
    38101 tcattacatt tggtggcctg ttaaccgaca gtcaacgcat tctcgatgcc gcagaaagca
    38161 aagaagggcg ctttattggt atcaatgcgc cgcgttctgg cgcttatgac aacggtttcc
    38221 aggtcgtgca tatgggctat ggcgtcgata aaaaagtgca ggtgccacaa aaactgtatg
    38281 aagcaggcgt gccaaccgtg ctggtgggta aggtggcaga tatcgtcaac aatccttatg
    38341 gcgtgagctg gcaaaatctg gtggatagcc agcggattat ggatatcacc ctcaacgaat
    38401 ttaacaccca tccgacggcg tttatttgca ccaacattca ggaaaccgac ctcgctggtc
    38461 atgcagaaga cgtcgcacgt tatgccgaac gtttgcaggt cgttgaccgt aaccttgccc
    38521 ggcttgttga ggcgatgcag ccagatgatt gcctggtcgt gatggcggat cacggcaacg
    38581 atccgaccat tggtcacagc caccataccc gcgaagtggt gccagtgctg gtttatcagc
    38641 aagggatgat cgctacgcag ctcggtgtgc gcaccacgct ttctgatgtg ggggctaccg
    38701 tgtgtgaatt tttccgcgcg ccaccgccac aaaatggtcg ctcttttctt tcctccctcc
    38761 ggtttgcagg agacacccta tgagttttga tccgacgggt tacaccctcg cccatgagca
    38821 tctgcatatt gatctctccg gctttaaaaa caacgtggac tgccgccttg atcagtatgc
    38881 gttcatttgc caggagatga acgacctgat gacccggggc gtgcgtaatg tgattgagat
    38941 gaccaaccgt tacatggggc gcaatgcgca atttatgctt gatgtaatgc gcgagacggg
    39001 gatcaacgtg gtggcctgta ccggttatta ccaggacgcg tttttcccgg aacatgtggt
    39061 gacccgcagc gtgcaggaac tggcgcagga gatggtcgat gaaattgaac agggtatcga
    39121 tggcacggag ctgaaagccg ggatcatcgc ggagatcggc accagcgaag gaaagattac
    39181 gccgctggaa gagaaggtat ttattgccgc tgcgctggcg cataaccaga ccggacgccc
    39241 gatctccacg catacgtcgt tcagcacgat ggggctggag caactggcgt tgctacaagc
    39301 ccacggggtt gatctttcgc gcgtcaccgt tggtcactgc gatctgaaag acaacctcga
    39361 caacattttg aagatgatcg atctcggcgc gtacgtgcag ttcgacacca ttggtaagaa
    39421 cagttactac ccggacgaaa agcgtattgc gatgcttcat gcgctacgtg accgtgggtt
    39481 gctgaaccgc gtcatgctgt cgatggatat tacgcgccgc tcccatttaa aagccaatgg
    39541 tggttatggc tatgactatt tattgaccac ctttattccg caattgcgcc agtcaggatt
    39601 cagtcaggcc gatgtggatg tgatgttacg tgaaactccc tctcaatttt tccaataagg
    39661 acagactcat gaaaaagatt ggcgttgcag gcttacagcg tgagcagatt aaaaaaacca
    39721 ttgaagcgac ggctcctggc agttttgaag ttttcattca caacgacatg gaagcggcaa
    39781 tgaaagtgaa atccgggcaa ctggattact acatcggtgc gtgtaatacc ggtgcgggcg
    39841 cggcattgtc gattgccatc gcggtgattg gctataacaa aagttgcacc attgccaaac
    39901 caggcattaa agcgaaagac gagcatatcg ccaaaatgat cgctgaagga aaagtggcgt
    39961 ttggcctttc cgttgagcac gtcgaacacg cgattccgat gctgattaac catctgaaat
    40021 aaaaggcacg actatggatc tgtatattca gattatcgtg gtggcgtgcc tgacgggtat
    40081 gacatcgctt ctggcgcatc gctcggcggc tgtttttcat gacggcatcc gcccgatcct
    40141 gccgcaactg attgaaggct atatgaaccg tcgcgaggcg gggagtatcg cttttggtct
    40201 gagcattggt tttgtggcct cggtggggat ctcttttacc ctgaaaaccg ggctgctcaa
    40261 cgcatggtta ctctttcttc ctaccgatat cctcggcgtc ctggcgataa acagcctgat
    40321 ggcgtttggt cttggcgcta tctggggcgt gttgatcctt acttgcctgt tgccagtaaa
    40381 ccagctgctg accgcgctgc cggtggatgt attaggtagc ctgggggaat taagctcgcc
    40441 ggtggtttca gcttttgcac tgttcccgct ggtggcgatt ttctaccagt ttggctggaa
    40501 gcaaagtctg atcgccgccg tggtggtact gatgacccgt gtggtagtcg tgcgctattt
    40561 cccacatctt aaccctgaat ctatcgaaat ctttattggc atggtgatgc tgctggggat
    40621 cgcgataact cacgacctgc gtcatcgtga tgaaaatgac attgatgcca gcgggctttc
    40681 ggtgtttgaa gaacgcacgt cacggattat caaaaactta ccctatatcg ccatcgtggg
    40741 agcattgatt gccgccgttg ccagcatgaa gatttttgct ggcagtgaag tgtcgatctt
    40801 cacactggag aaagcatatt ccgcaggcgt aacgccggaa caatcgcaaa cgctgattaa
    40861 tcaggcggct ctggcagaat ttatgcgcgg actggggttt gtgccgttga ttgccaccac
    40921 cgcgttagca acgggtgtgt atgcagttgc gggctttacc tttgtttatg cggtgggcta
    40981 tctctcgccg aatccgatgg ttgcagcggt attaggtgca gtggttattt cggcagaagt
    41041 tttattgctt cgttcgatcg gcaaatggct ggggcgctat ccgtcggtgc gtaatgcgtc
    41101 ggataacatc cgtaacgcca tgaatatgct gatggaagtg gcgctgctgg tcggttcgat
    41161 tttcgcagca attaagatgg cgggttatac cggattctct atcgcggttg ccatttactt
    41221 cctcaacgaa tccctgggcc gtccggtaca gaaaatggcg gcaccggtcg tggcagtaat
    41281 gatcaccggt attctgctga atgttcttta ctggcttggc ctgttcgttc cggcttaagg
    41341 aaggcaccta tgaagacgtt tcctctgcaa agcctgacga ttattgaggc gcagcaaaag
    41401 cagtttgcgc tggtggatag catttgtcgc catttccccg gcagcgagtt tcttactggc
    41461 ggtgatttag gcttaacgcc aggactgaat caaccgcgcg ttacccagcg tgtggaacag
    41521 gtgctggctg atgcatttca cgcacaggct gcggcgctgg tgcagggcgc ggggactggc
    41581 gcgattcgcg ccgggctggc ggctttgctc aaaccggggc agcgtcttct ggtgcatgac
    41641 gcgcctgttt acccgacgac acgggttatt attgagcaga tggggctgac gcttattact
    41701 gttgatttca atgacctgtc ggcactgaag caggtcgtcg acgagcaaca accggatgcg
    41761 gcgctggtgc agcatacgcg ccagcagccg caggacagct acgtgctggc agatgtgctg
    41821 gcaacgttgc gcgcggcagg tgttccagcg ttaaccgatg acaactatgc ggtgatgaag
    41881 gtggcgcgaa tcggctgtga atgcggcgcg aatgtctcga cattttcctg cttcaagcta
    41941 tttgggccag agggtgttgg tgcagtggtc ggcgatgctg atgttatcaa ccgtattcgc
    42001 gccacgcttt actccggcgg tagccagatc cagggcgcac aggcgctgga agtattgcgt
    42061 ggtctggtgt ttgcgccagt gatgcacgcg gtgcaggcag gggtatctga acggttgctg
    42121 gctttgctta acggtggtgc ggtgccggaa gtgaaaagcg cggtgattgc taatgcgcag
    42181 tcgaaggtgt tgattgtcga gtttcatcag ccgattgccg ccagagtgct ggaagaggcg
    42241 caaaagcgcg gtgccttgcc ttacccggtg ggtgcagagt cgaaatatga aatcccgccg
    42301 ctcttttatc gcctttccgg aacgtttcgc caggcgaatc cacaatcaga acattgtgcg
    42361 attcgcatta acccgaatcg cagcggtgaa gagacggtgc tgcggatttt gcgtgagagt
    42421 attgccagta tttaatcttg tgaatgatgt tttcgaaaag aatgcagaaa caaatacgcc
    42481 ctcggaaaat ccagagggcg tcgggcaatt aaaccggtgt tagccgattt ctgtcagaga
    42541 cttactgtgc agtaggaccg caaccgccaa tgattttaga aatagaaatt gccgggtgca
    42601 gcaggtagtc gtaagagcag ttcttatcac ggttttcaat atgaccagta catgcagtta
    42661 acgtagccag tactgcacct accagtgcaa ctttaataaa tttg
//
LOCUS       DAPHDX010000026        42042 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPHDX010000026 DAPHDX010000000
VERSION     DAPHDX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.026"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..632
                     /gene="sxy"
                     /locus_tag="RH058_001365"
     CDS             3..632
                     /gene="sxy"
                     /locus_tag="RH058_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV3136971.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(595..2757)
                     /gene="yccS"
                     /locus_tag="RH058_001366"
     CDS             complement(595..2757)
                     /gene="yccS"
                     /locus_tag="RH058_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV3136972.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDEAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(2767..3213)
                     /locus_tag="RH058_001367"
     CDS             complement(2767..3213)
                     /locus_tag="RH058_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV3136973.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            3336..5390
                     /gene="helD"
                     /locus_tag="RH058_001368"
     CDS             3336..5390
                     /gene="helD"
                     /locus_tag="RH058_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV3136974.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(5422..5880)
                     /gene="mgsA"
                     /locus_tag="RH058_001369"
     CDS             complement(5422..5880)
                     /gene="mgsA"
                     /locus_tag="RH058_001369"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV3136975.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5976..6638)
                     /locus_tag="RH058_001370"
     CDS             complement(5976..6638)
                     /locus_tag="RH058_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV3136976.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6811..7224
                     /locus_tag="RH058_001371"
     CDS             6811..7224
                     /locus_tag="RH058_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV3136977.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7269..7586)
                     /gene="hspQ"
                     /locus_tag="RH058_001372"
     CDS             complement(7269..7586)
                     /gene="hspQ"
                     /locus_tag="RH058_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV3136978.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7644..8834)
                     /gene="rlmI"
                     /locus_tag="RH058_001373"
     CDS             complement(7644..8834)
                     /gene="rlmI"
                     /locus_tag="RH058_001373"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV3136979.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8929..9207
                     /gene="yccX"
                     /locus_tag="RH058_001374"
     CDS             8929..9207
                     /gene="yccX"
                     /locus_tag="RH058_001374"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV3136980.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIH"
     gene            complement(9204..9533)
                     /gene="tusE"
                     /locus_tag="RH058_001375"
     CDS             complement(9204..9533)
                     /gene="tusE"
                     /locus_tag="RH058_001375"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV3136981.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(9624..10283)
                     /gene="yccA"
                     /locus_tag="RH058_001376"
     CDS             complement(9624..10283)
                     /gene="yccA"
                     /locus_tag="RH058_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV3136982.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(10490..10577)
                     /locus_tag="RH058_001377"
     tRNA            complement(10490..10577)
                     /locus_tag="RH058_001377"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10541..10543),aa:Ser,seq:tga)
     gene            11004..12122
                     /gene="hyaA"
                     /locus_tag="RH058_001378"
     CDS             11004..12122
                     /gene="hyaA"
                     /locus_tag="RH058_001378"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV3136983.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12119..13912
                     /gene="hyaB"
                     /locus_tag="RH058_001379"
     CDS             12119..13912
                     /gene="hyaB"
                     /locus_tag="RH058_001379"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV3136984.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLVTASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13931..14638
                     /gene="hyaC"
                     /locus_tag="RH058_001380"
     CDS             13931..14638
                     /gene="hyaC"
                     /locus_tag="RH058_001380"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV3136985.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14635..15222
                     /locus_tag="RH058_001381"
     CDS             14635..15222
                     /locus_tag="RH058_001381"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV3136986.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            15219..15617
                     /gene="hyaE"
                     /locus_tag="RH058_001382"
     CDS             15219..15617
                     /gene="hyaE"
                     /locus_tag="RH058_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV3136987.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            15614..16471
                     /locus_tag="RH058_001383"
     CDS             15614..16471
                     /locus_tag="RH058_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV3136988.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            16605..18149
                     /gene="appC"
                     /locus_tag="RH058_001384"
     CDS             16605..18149
                     /gene="appC"
                     /locus_tag="RH058_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV3136989.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAITETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18161..19297
                     /gene="appB"
                     /locus_tag="RH058_001385"
     CDS             18161..19297
                     /gene="appB"
                     /locus_tag="RH058_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV3136990.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            19310..19402
                     /gene="cbdX"
                     /locus_tag="RH058_001386"
     CDS             19310..19402
                     /gene="cbdX"
                     /locus_tag="RH058_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV3136991.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            19482..20780
                     /gene="appA"
                     /locus_tag="RH058_001387"
     CDS             19482..20780
                     /gene="appA"
                     /locus_tag="RH058_001387"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV3136992.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLRAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQADGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20895..23075)
                     /gene="etk"
                     /locus_tag="RH058_001388"
     CDS             complement(20895..23075)
                     /gene="etk"
                     /locus_tag="RH058_001388"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV3136993.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23095..23541)
                     /gene="etp"
                     /locus_tag="RH058_001389"
                     /gene_synonym="yccY"
     CDS             complement(23095..23541)
                     /gene="etp"
                     /locus_tag="RH058_001389"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV3136994.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(23529..24668)
                     /locus_tag="RH058_001390"
     CDS             complement(23529..24668)
                     /locus_tag="RH058_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV3136995.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24714..26810)
                     /locus_tag="RH058_001391"
     CDS             complement(24714..26810)
                     /locus_tag="RH058_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV3136996.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(26810..27556)
                     /locus_tag="RH058_001392"
     CDS             complement(26810..27556)
                     /locus_tag="RH058_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV3136997.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(27553..28197)
                     /gene="gfcB"
                     /locus_tag="RH058_001393"
     CDS             complement(27553..28197)
                     /gene="gfcB"
                     /locus_tag="RH058_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDV3136998.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(28304..28609)
                     /locus_tag="RH058_001394"
     CDS             complement(28304..28609)
                     /locus_tag="RH058_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HDV3136999.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(29051..29263)
                     /gene="cspH"
                     /locus_tag="RH058_001395"
     CDS             complement(29051..29263)
                     /gene="cspH"
                     /locus_tag="RH058_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV3137000.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            29549..29761
                     /gene="cspG"
                     /locus_tag="RH058_001396"
     CDS             29549..29761
                     /gene="cspG"
                     /locus_tag="RH058_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV3137001.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            29772..29960
                     /locus_tag="RH058_001397"
     CDS             29772..29960
                     /locus_tag="RH058_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV3137002.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQICTDHAPLDFTK"
     gene            29935..30165
                     /gene="ymcE"
                     /locus_tag="RH058_001398"
     CDS             29935..30165
                     /gene="ymcE"
                     /locus_tag="RH058_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV3137003.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            30155..30328
                     /locus_tag="RH058_001399"
     CDS             30155..30328
                     /locus_tag="RH058_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV3137004.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(30376..31449)
                     /locus_tag="RH058_001400"
     CDS             complement(30376..31449)
                     /locus_tag="RH058_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV3137005.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLATNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(31521..34265)
                     /gene="torS"
                     /locus_tag="RH058_001401"
     CDS             complement(31521..34265)
                     /gene="torS"
                     /locus_tag="RH058_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV3137006.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGQMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            34348..35376
                     /gene="torT"
                     /locus_tag="RH058_001402"
     CDS             34348..35376
                     /gene="torT"
                     /locus_tag="RH058_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV3137007.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(35349..36041)
                     /gene="torR"
                     /locus_tag="RH058_001403"
     CDS             complement(35349..36041)
                     /gene="torR"
                     /locus_tag="RH058_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV3137008.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            36171..37343
                     /gene="torC"
                     /locus_tag="RH058_001404"
     CDS             36171..37343
                     /gene="torC"
                     /locus_tag="RH058_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV3137009.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            37343..39889
                     /gene="torA"
                     /locus_tag="RH058_001405"
     CDS             37343..39889
                     /gene="torA"
                     /locus_tag="RH058_001405"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV3137010.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            39886..40485
                     /gene="torD"
                     /locus_tag="RH058_001406"
     CDS             39886..40485
                     /gene="torD"
                     /locus_tag="RH058_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV3137011.1"
                     /translation="MTTLTAQQIACIYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNALENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(40578..40883)
                     /gene="cbpM"
                     /locus_tag="RH058_001407"
     CDS             complement(40578..40883)
                     /gene="cbpM"
                     /locus_tag="RH058_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV3137012.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(40883..41803)
                     /gene="cbpA"
                     /locus_tag="RH058_001408"
     CDS             complement(40883..41803)
                     /gene="cbpA"
                     /locus_tag="RH058_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV3137013.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        10171 a        10699 c        11084 g        10088 t
ORIGIN      
        1 ttatgaaaag cctctcctat aagcggatct ataaatcaca agaatacctg gcaacgttgg
       61 gcacaattga ataccgatca ttgtttggca gttacagcct gaccgttgac gacacggtgt
      121 ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg tgagcaaagt gcacagtact
      181 gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg tggccgatcc gttaccctca
      241 attactatcg ggttgatgaa agtctatggc gaaatcaact gaagctggtg cgtctgtcga
      301 aatattctct cgatgcagcg ctgaaagaga aaagcacgcg caatacccgg gaaagactga
      361 aagatttgcc caatatgtct tttcatctgg aagcgattct cggggaggtg gggattaagg
      421 atgtacgggc gttacgtata cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa
      481 acagtctggt gacagaaaag attctgttta tgcttgaagg tgccattatc ggcattcatg
      541 aagctgcgct cccggtggca cgccgccagg agcttgcaga atgggctgac tctcttacgc
      601 cgaaacagga gtttcctgcg gaacttgagt aatctggcgt tgcagacgac caatctcagg
      661 cagtaatgca atcaataatc caacttgttg tacgaccaga ggttctttgc tgtctgcccg
      721 tggttcaagt tgctgcatcc gctgtttcag gctagctaat gcctcattga cgcgttcttc
      781 atcagcaggt tgatgatgta acgcgtcatc aacatagcaa actgcctcat caagaaacgc
      841 cagaatttca ggattagtta actgctcccg gtgagcaccg agggctgaga tatagctggt
      901 aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg cgaatttgcg gggtaacgtt
      961 cggttcgctg gacatatttg ataccaccga cgccagttca gcatcacggt tgtgtgcatc
     1021 gcggcgggca atacgatacg ccagacggtt atcacgcccc tgatggtatt gctccagtat
     1081 ggcatcgaga taccggcagt tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg
     1141 aaactgccag tcaggccaga tgtagctcac tgccgcccac gcaatggcac aaccaatcag
     1201 cgtatcgatt acgcgaggta acgctacttc aaaaccttca cccagcaagt taaaacacag
     1261 tagcaccaaa agtgtgatga acatcgttgc atgggcatat tgcacgttac ggaaggcaaa
     1321 aaagagcacg ccggtaataa ccagtagcac cagctgccct tccagtgacg gtacaaacca
     1381 cagcacagga atgccaatgg cgataccgac cagcgtacca ataatcctta acttcaggcg
     1441 gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa cttgtcagca agatccaata
     1501 cccgtgatgc attccggtta tttgaatgat ggcgtagccg aagcacaaca ccagcgacat
     1561 tcttatcgca tgacggaaga gggcggattc cggcgtgaag tgacggctaa gacgcagcca
     1621 gatatcactc aacccgtgcg ggctgtcatc agcgagctca ttttcgtcat tattatgggg
     1681 tagtgcctgg gcctgttctg attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt
     1741 gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt gcgccgttat cgcgcatccg
     1801 ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa tgcggatcat gttgataagg
     1861 ctgacgcaac aaaatacagc gtgacagttg ctggcacgcc tggccctgca tcgacatcag
     1921 ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg
     1981 aatatgagaa gagctggcac gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt
     2041 gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt
     2101 ctgattcaat gtcgccatca gcagaccgtt ggcgagagcc aaatcgtaca gcggtgcctg
     2161 gctttcatct tcaatatcag gatcaaacat gcgcgacttg agctcaagat aacgcgccag
     2221 ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg accgggaaca gcagatgacc
     2281 aataagtgtc aggacgttgt accagacggc accggccagc agatacatcg gctgctgata
     2341 ccagtgctca tacagtgatg ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa
     2401 ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa atgaagccgc tggtagagag
     2461 cgttaagcca atcgcaaata gccagggcca gggaaacagc aattctactg aggccgaggc
     2521 gataaaaaag cagaacagcg taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc
     2581 gagatcggtc agcgccgctg ccaccatccc cagcgttagc ggaatcgtca gttttacatc
     2641 acccagccac cacggaaacg ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata
     2701 cagccaggcg ctgttccagg tatagcgttt gagcaaagga cttagcataa aggccatatt
     2761 agtggattat tcaaaacgac gacgtgcatt ggcttcacgc gcagcttgcg ccgtttctac
     2821 cgataccacg cgacgaccaa ccggccatag tgcaatagcg gcaattttaa agttcgcaat
     2881 gccgacagga atgccaatga ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag
     2941 gcataaccac cagccaaaga aaatcagcca gaaaatattc aataccgtac cgccagtatt
     3001 cagcagcaca tttttgccag ccgggttcag ttcatcgaca tgaatagctt cattgccata
     3061 aggcaccaga gacagtttag tgatctccca gcaggatcgt gtcagcggta aggtaaaaat
     3121 cagcataata ctgaccagag tcgccaacag ccagcccaga gtggtggcaa atccgccaag
     3181 cacaaagttc agaatgttca aaacggtacg cataaaacct cgctttactg tggttttcag
     3241 taatgggcgg caattgtaac gtttttttgg gctggagcac gttttctctg acggttacac
     3301 tgataagaaa taatttcgtg tggatctaca gagtcatgga actgaaagcg acaacgcttg
     3361 gaaaacgtct ggcacagcac ccttacgatc gggcggtgat cctcaatgcc gggattaaag
     3421 tctccggcga tcgccacgaa taccttattc ctttcaatca attactggcg attcactgta
     3481 agcgcggtct ggtatggggc gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc
     3541 tgcacggcac cgaatggggc gagacgcagc gtttttacca tcatcttgat gctcactggc
     3601 agcggtggag tggcgagatg agcgaaattg cgtctggtgt tttacgccag caactggatt
     3661 tgattgccac gcgcactgga gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc
     3721 agcaacaaat ccgccaggct atgtcggcgt tgccgttgcc ggttaaccga ctggaagaat
     3781 tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg gttgaaagat attgaaagcg
     3841 ctcggttgca gcataaccag gcgtataccg aagccatgct taccgagtat gcggattttt
     3901 tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg
     3961 agcattctct gttagtgctg gcaggtgcag gaagcggaaa aacgtcggtg ctggtggccc
     4021 gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt
     4081 ttggtcgcaa agccgctgaa gagatggacg agcggattcg cgaacggcta cataccgaag
     4141 acattaccgc acgcacgttt catgcgctgg cgctgcatat tattcagcag ggcagcaaaa
     4201 aagttccgat agtcagcaaa ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg
     4261 ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc
     4321 tgacggaaga aatgcagtgg tcagtgccag aaggtaactt ctgggatgat gaaaaattac
     4381 agcgtcgtct tgcctcacgc ctcgatcgtt gggtaagtct gatgcggatg cacggtggtg
     4441 cacaggcaga aatgattgcc agtgcacccg aagagattcg cgatctgttc agtaaacgta
     4501 tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg
     4561 tcgatttttc gggccttatt catcaggcga ttgtgattct ggagaaaggt cgctttatca
     4621 gcccgtggaa gcatattctg gttgatgaat ttcaggatat ctcgccgcag cgggcagcgt
     4681 tgttagcggc attacgcaag caaaacagtc agacgacgtt gttcgccgtt ggtgatgact
     4741 ggcaggcgat ttaccgattc agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa
     4801 actttggtga aggcgatcgc tgtgatttag acacgactta ccgttttaac agtcgtatcg
     4861 gtgaggtggc aaaccggttt attcagcaga acccaggcca gttgaaaaag ccgctaaaca
     4921 gcttaaccaa tggagacgaa aaagccgtca cgttattgga tgagagtcaa cttgacgctt
     4981 tgctggataa gctctctggt tatgccaaac cggaagagcg cattcttatc ctggcgcgtt
     5041 accatcacat gaggcctgcc agcctggaaa aagcggcaac acgctggccg aagttgcaaa
     5101 tcgactttat gaccattcat gccagcaaag ggcaacaggc ggattacgtc atcatcgttg
     5161 gcttgcagga gggaagtgat ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg
     5221 cgctactgcc accggttgag gatttcccgg acgctgaaga acggcggtta atgtacgtgg
     5281 cgctgacccg ggcacgccat cgggtatggg cactgtttaa caaagagaat ccctctccct
     5341 ttgtggaaat actgaaaaat ctggatgtgc cggtagcgag aaaaccgtaa gaaacaggtg
     5401 gcgtttgcca cctgtgcaat attacttcag acggtccgcg agataacgct gataatcggg
     5461 gatcagaata tcgaccgcgt cgttgaaatg cggcgactgg attatgaagt ctgccgttgc
     5521 cacgttggtg gcgaccggaa tgttccatac cgtcgccaga cgcaacaagg ctttcacgtc
     5581 aggatcgtgc ggcacggcat ttagtgggtc ccagaagaaa atcaatacat caattttccc
     5641 ttctgagatc aatgcgccaa cctgctggtc accccccatt gggccactca acatcgcgtt
     5701 gacgttcatg ccggtcgcgc gggaaattaa gttaccggta gtgcctgttg catacagtac
     5761 gtgttgttcc agtaacggtt gatgccgttc cacccagctc atcagcattt gtttgcagtg
     5821 atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat
     5881 aatgtacatc cgtagttaac tttcctacag attactgtaa gcacttatcg ctgcaagata
     5941 aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg aagatggttg tttttccgcc
     6001 cactgtagga aagtatttcg cgtttgtggg tcagccagtt taaaccaata cttcagccgt
     6061 tgttctgtga gcacctgaga ctgcggtgga atagcatcca gctcggaaac acccgaaagc
     6121 agcgtactgt catctgccat catcgtggca aattgcggta gtgaagcgcg tttcgcggat
     6181 ttgttatagc gttcaacctc gacctcataa tcaatcgttt ttgccgttga ggtaatggcg
     6241 agaatatcca gctttaccgg aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc
     6301 gcggcatcaa aatggttagc ttcccgctca ttctccaggc gaggcaggcg aaaattgacc
     6361 tggttgatga gctgggtatt aaaactgacc accagcggtg gggagatata cagccgttct
     6421 tcactattgg aaagatgaat cgtcttctca acgcgaaaca ctaactgatg cggtccatta
     6481 tccagttcaa tgctgtccgc accgcgcagc agagagctgg aaaccttctt gccgtcgagt
     6541 accagaagat cgacatcggt tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa
     6601 cataatgcaa tcaaggtggt cacgatgccg gttttcatag gtagctcctg tcaaaagacc
     6661 gcccgaataa taatgtgtca aaatttttct ttaatcatac ttactaacat atagacatat
     6721 ttcccgcttt tgctctcatt cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg
     6781 tacactttgt taaacagtct ggaggaaata atgaaagaaa ccgatattgc aggcatttta
     6841 acgtccactc acaccattgc gctggtgggg gcaagcgaca aacccgatcg tcccagctat
     6901 cgcgtgatga aatatctact cgaccagggg tatcacgtca ttccggtttc gccaaaagtt
     6961 gccggcaaaa cgctgctggg ccagcagggg tatggcacgc tggcagacgt tccggaaaag
     7021 gtcgatatgg tcgatgtttt ccgtaattca gaagcggcgt ggggcgtggc acaggaagct
     7081 attgccattg gcgcgaaaac cctctggatg caactggggg tgattaatga gcaagcggca
     7141 gtactggcac gggatgccgg gttgaatgtg gtgatggatc gctgcccggc tattgagatc
     7201 cctcgcctgg gcctggccaa ataaaaaatc cccggaaggc aaaaaccttc cggggattcg
     7261 ttcagggatc agttacgcag acgcggggcc tggagttgtt tgcggatggt ctgcgccagc
     7321 tcatccatgg atggctgttc aggatgttca tcctgcaatt cgctacttag ctgggcttca
     7381 gccaggtagg tatgaaccgg taggccgtta tcgtcctcca tcactacgtg ataccacgga
     7441 gcggcgcgaa gctcgtcatt taccgccagc tcatcaggcg acggttcaga gagcgaataa
     7501 accgggtcga tatccacgac cactccgaga taacctaaca gggaatggcg gacctgctgg
     7561 ccgataccga atttgctggc aatcatagtc acctcccggg aaatctttac ctttgatata
     7621 agggtaaaat tccacatttc aagttacatg acgcgacagg caaacccttt cagatatagc
     7681 ccttccggat aggtagcgat caccggatga tcggctgcct gacggaactg ctctataaat
     7741 tgtacatcac ggccggcatc aattgcggca tccgcgatga ttttctgaaa taaatcgctg
     7801 gtcatcagac cggaacagga gaaagtcagg agaataccgc cttcattcag caactgaatc
     7861 gccagcatgt tgatatcttt atagccacga cacgcgccca tcaactggct tttattctca
     7921 acaaacttcg gcgggtccat cacgataacg tcaaattttt caccgcgatc gcgataagta
     7981 cgcagcaatt taaagacatc atcacggaca aactcagcct tgctcagatc cagtttgttc
     8041 agctcaacgt tctgccgtgc aatatccagc gcttcctggg aggtatcaac gctgacaacc
     8101 tggctgcaac cgcccatcag tgccgatacg gcgaaaccac cggtatagga gaaacagttc
     8161 agcacacgtt tattttcaac gtagcggcgg gtagccaggc ggctatcacg ctggtccagg
     8221 tagtagcccg ttttgtgtcc gtgctgaata tccaccagca gtttcattcc gtgttcttca
     8281 atcggcagca gggcaggtgg caactcgccg gtgacggggc cctgggtcag ctccattcct
     8341 tcttttttac gtaccgcaac atcgctgcga tcgtaaatcg aacattccgg gtacagcgtt
     8401 tgcagggcac taattaatgc cgcgcgctga tattctgccc cagcactcag cagttgcagc
     8461 accagaaaat taccgaaacg atcgatagta atacccggca ggccatcaga ttctccggcg
     8521 attaaacgat agctgtcgag gccatctttt tgcgccagcc agtcacgcca tttttgtgct
     8581 tgttgcaaac ggcgggaaaa aaaagcaatg tcgatagact cagacgggtc aaacgtccag
     8641 acgcgcgccc ggatttgcga agctggcgaa taagcgccgc gtgctaacca ttttccctga
     8701 tgatcaacaa tatcgatggt ttcaccgagg ctggctttac cttccatgcg ggcaacggcc
     8761 ccggaaaaga cccacggatg gcgacgaagt aatgattttt cgcgcccttt ggctaacact
     8821 aaacgtacac tcataattta cttttctgtc gatgcaaaga aatgggcgtc attgtccgga
     8881 gttttccatg gatttgcaac gcgtccatta aggataagga gagcagcaat gtcgaaagtc
     8941 tgcataattg cctgggttta cgggcgagtt cagggcgtag gatttcgcta caccacacag
     9001 tacgaagcga aaagactggg tttaaccggg tacgccaaaa atcttgacga tggcagcgtg
     9061 gaagtggttg cttgcggtga agaagggcag gtggaaaaat taatgcagtg gttaaagagt
     9121 ggcggcccgc gttctgcgcg tgtggaacgg gtgcttagcg agccgcatca tccctcgggg
     9181 gaattagccg attttcgtat ccattaaata cattttaccg gttttggcag gccagcaatt
     9241 ttggtggctt gcttagccgg acctttcgga aacagtcgat ataaatagcg gctgttacct
     9301 ttttcttcgc caaatttatt cgccatcgct tttaccagca tacgaatcgc cggagaagta
     9361 ttgaattcca gatagaaatc acgcacaaaa cgcaccactt cccagtgttc tggcgacagc
     9421 gaaatccctt cgttctctgc aatcaccaca gccagcggct cactccactg gctgctttct
     9481 ttgagatagc cttcggtatc cgtttctatc tctttacctt cgaagatcag cataattatt
     9541 actaccttaa tcagactggc ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc
     9601 tatgaaagtg ttagcgggtg agattaatcg cggctggcga agcccagaat gctcagcagg
     9661 ctgacgaaga tgttgtacag cgaaacatac aggctaaccg tggcacgaat atagttcgtt
     9721 tcaccgccat gaatgatgtt gctggtttca aacaaaatag cgccagagga gatcaaaatg
     9781 aagaccgcgc tgatcgccag atgcagagca ggcagttgca ggaagatatt agcaaccata
     9841 ccaatcagca ccaccacaat acccgccatc agcataccgc cgaggaacga catatctttg
     9901 cgggtggtca gcacatatgc agagcagcag aagaacacta acgccgttcc gcccagtgcc
     9961 atagcgatta cgtcacccat tccggcagac agataggtgt tcagaattgg tccgaggata
    10021 taacccagaa aaccggtaaa ggcgaatgcg gagataatcc cggtcggctt attcgccgtt
    10081 ttataggtca ggaacatcaa accatacata cccaccagcg tcagaatcag acccggagat
    10141 ggcagcatca gcaccgtact ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc
    10201 agcagaaaat aggtattacg cagcacctta tgggtgctaa gcagtgatgt acggtcatgt
    10261 gaagaactaa caatacgatc cattgagtca ctctctatga cagatgtaat taattaagca
    10321 gcataatgat aatgcgtaag ggcacccaga agttttaccc atctttacgc atttgatctg
    10381 gaacaggttt aacagcggat tatcaggtca ttaagcaaat ataacgccct gagaatttcg
    10441 acaggcaaaa gaaaaagggg ttagcattta gctaacccct tatcttattt ggcggaagcg
    10501 cagagattcg aactctggaa ccctttcggg tcgccggttt tcaagaccgg tgccttcaac
    10561 cgctcggcca cacttccgga atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc
    10621 cctaatttgt ttgtttgcct gaaaaacagc caaaagtgca ttgatagcgt gaaataacag
    10681 cagattgatc atttcatcac catgaattcc ttctctttta ctcgtttagc aaccggctaa
    10741 acatccccac cgcccggcca aaagaaaaat aggtccattt ttatcgctaa aagataaatc
    10801 cacacagttt gtattgtttt gtgcaaaagt ttcactacgc tttattaaca atactttctg
    10861 gcgacgtgtg ccagtgcaga aggatgagct ttcgttttca gcatctcacg tgaagcgatg
    10921 gtttgccttg ctacagggac gtcgcttgcc gaccatgtgc gcccggtgtc ctgccggtgt
    10981 cgcaaggaga agagacgtgc gatatgaata acgaggaaac attttaccag gccatgcggc
    11041 gtcagggcgt tacccggcgc agctttctca aatattgtag tctggctgcc acgtcgctgg
    11101 gattaggcgc gggaatggca ccaaagattg cctgggcgct ggagaacaaa ccccgcattc
    11161 cggtggtatg gatccacggt ctggaatgca cctgctgtac cgaatctttt atccgctccg
    11221 ctcacccact ggcgaaggac gtcatccttt ccctgatttc cctcgattac gacgatactt
    11281 tgatggctgc cgccggaacc caggcggaag aagtctttga agacatcatc gcgcaataca
    11341 atggcaaata tatcctcgca gtagaaggta atccgccgct gggcgagcag gggatgttct
    11401 gtatcagcag cggtcgaccg tttattgaga aactcaaacg tgccgctgcc ggagccagcg
    11461 cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt gcaggccgcg cggcccaatc
    11521 cgacgcaggc aacgcctatc gacaaagtca tcaccgacaa acccattatc aaagtacctg
    11581 gctgcccgcc gatcccggat gtgatgagcg ccatcattac ttacatggtg acctttgatc
    11641 gcttgccaga tgtcgacaga atgggccgtc cattgatgtt ctatggtcag cgaatccacg
    11701 ataaatgcta tcgccgcgcc cacttcgacg ccggagagtt cgtccagagt tgggatgatg
    11761 acgctgcccg caaaggttac tgcctgtaca aaatgggttg caaagggcca accacctata
    11821 acgcctgttc ctccacccgc tggaatgatg gcgtttcttt cccaatccag tctggtcacg
    11881 gctgcctggg ctgtgcggaa aatggtttct gggatcgcgg ttcgttctac agccgcgtgg
    11941 tcgatattcc gcaaatgggt actcattcca ctgccgatac cgtcggttta accgcgcttg
    12001 gcgtggtggc agcggctgtt ggtgtgcacg cagtcgccag cgccgttgac cagcgcagac
    12061 gtcataacca gcaacctaca gaaaccgaac atcagccagg caatgaggat aaacaggcat
    12121 gagcactcag tacgaaactc agggatacac tatcaataat gccggacgcc gcctggtggt
    12181 cgacccgatt acgcgcatcg aaggccacat gcgctgcgaa gtgaatatta acgatcagaa
    12241 tgtgatcacc aatgccgtct cctgcggcac catgtttcgc gggctggaga tcattctgca
    12301 agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg
    12361 cgtacacgcc ctggcttcgg tttacgccat cgaagatgct atcggtatta aagtgccgga
    12421 caacgccaat atcatccgca acattatgct ggcaacgctc tggtgccacg atcatctggt
    12481 gcacttctat cagcttgccg ggatggactg gatcgatgtg ttagatgcgc tgaaagccga
    12541 cccgcggaaa acctccgaac tggcgcaaag tctctcctct tggccgaaat catccccggg
    12601 ctatttcttc gacgtacaaa accgcctgaa aaaattcgtt gaaggcgggc agttggggat
    12661 cttccgcaat ggctactggg ggcacccgca gtacaagttg ccgccagaag ccaacctgat
    12721 gggctttgcc cactacctcg aagctctcga tttccagcgt gaaattgtga aaatccacgc
    12781 ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc ggcgggatgc cttgcgccat
    12841 caacattgac gaaagcggcg cggtcggggc agtcaatatg gaacgcctga acctggtgca
    12901 gtcaattatc acccgcacgg cggacttcat taacaacgtg atgatccccg acgccttagc
    12961 catcggtcag ttcaacaaac cgtggagcga aatcggcact ggtctttctg ataaatgcgt
    13021 tctcagctac ggcgcattcc cggatattgc caacgacttt ggcgagaaaa gtctgctgat
    13081 gcctggcggc gcggtgatta acggcgactt caacaatgtg ctgccagtgg atttggttga
    13141 tccgcagcag gtgcaggagt ttgtcgacca cgcctggtat cgatatccca acgatcaggt
    13201 cgggcgtcat ccgttcgatg gcatcaccga cccgtggtac aaccccggcg atgtcaaagg
    13261 cagcgatacc aacattcagc agctgaatga acaggaacgc tactcgtgga tcaaagcgcc
    13321 acgctggcgc ggtaacgcga tggaagtggg gccgctggcg cgcacgttaa tcgcttatca
    13381 caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg atgtcggcgt tgaacctgcc
    13441 gctttccggt atccagtcaa cgttaggccg cattttgtgc cgcgcgcacg aagcgcagtg
    13501 ggccgcaggt aagttgcagt atttcttcga caagctgatg accaacctga aaaacggcaa
    13561 tctcgtcact gcttccacgg aaaaatggga acctgcaacc tggccgacag agtgccgtgg
    13621 tgtcggtttt accgaagcgc cgcgcggggc gttaggccac tgggccgcca ttcgcgatgg
    13681 caagattgat ctctaccagt gcgtggtgcc gaccacctgg aacgccagcc cgcgcgatcc
    13741 caaagggcag attggcgctt atgaagcggc gctgatgaac accaaaatgg cgatccccga
    13801 gcaaccgctg gagatcctgc gtactctgca cagctttgac ccgtgcctcg cctgttcaac
    13861 acacgtgctg ggcgacgacg gtagcgagct gatctccgtg caggtgcgtt aacagcgaag
    13921 gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc cactatgtct ttgaagcgcc
    13981 agtccgcatc tggcactggt tgacggtgtt atgcatggcg gtgttgatgg tcaccggata
    14041 ctttatcggc aagccgctac cttccgtcag cggcgaggcg acgtatctgt tctatatggg
    14101 ctacatcagg ttaattcact tcagcgccgg gatggttttt accgtggttt tgctgatgcg
    14161 gatctactgg gcttttgttg gcaatcgata ctcccgcgag ctgtttatcg tgccggtatg
    14221 gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc tggtatctgt ttctggcaaa
    14281 acgtccgagt gccgatatag gccataatcc catcgcccag gcggcgatgt tcggctattt
    14341 cctgatgtcg gtctttatga tcatcactgg ttttgcgctg tatagcgaac acagccagta
    14401 cgctattttt gcgccgttcc gttatgtggt ggaatttttc tactggatgg gtggcaactc
    14461 aatggacatt cacagctggc atcggctggg gatgtggctg attggcgcgt ttgtgatcgg
    14521 tcatgtctac atggcgctgc gtgaagacat catgtccgac gacacggtga tctccaccat
    14581 ggtcaacggc taccgtagcc acaaatttgg caaaataagt aacaaggagc gttcatgagc
    14641 gagcaacgcg tggtggtcat ggggctgggc aacctgctgt gggccgatga aggcttcggc
    14701 gtgcgggtgg cggaacggct gtatgcccat taccactggc ccgagtatgt ggagattgtc
    14761 gatggcggta ctcagggact gaacttgctg gggtatgtcg aaagcgccag ccatctgttg
    14821 attctcgatg ccattgacta cgggctggaa cctggaacgc tgcgaaccta tgccggagaa
    14881 cgcattccgg cttatctcag cgcgaagaaa atgagcctgc atcagaacag tttctccgaa
    14941 gtgttggcgc tggcggatat ccgcggacat ctgccagcac atattgccct cgtcggtctg
    15001 caacccgcaa tgctcgacga ctacggcggt agcctgagcg aactggcacg ggagcaactg
    15061 cccgctgcgg aacaggcggc gctggcgcag ctggctgcgt ggggaattgt gccgcaaccg
    15121 gctaatgaat cgcgctgtct caattatgac tgtctgtcga tggaaaatta cgaaggcgtt
    15181 cgcttgcgcc agtaccggat gacacaggag gagcagggat gagcaacgac acgccatttg
    15241 atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc agtcagtgaa tcccgtcttg
    15301 acgactggct tacgcaagcg ccagacggcg tgctgttatt aagcagtgac ccgaaacgca
    15361 cgccagaggt cagcgataat ccggtaatga ttggcgaatt actgcgcgag tttcccgact
    15421 atacatggca ggtggcgatt gctgaccttg agcagagcga agccatcggc gatcgctttg
    15481 gcgtctttcg ctttcctgcc actttagtgt ttaccggcgg aaactatcgc ggcgtgctga
    15541 atggtattca cccgtgggcg gaactgataa acctgatgcg cgggcttgtc gaaccgcagc
    15601 aggagcgtgc ctcatgagcg aaacattttt ccatctgctg gggccaggaa cgcaaccgaa
    15661 cgatgacagt ttcagcatga atccactgcc gatcacctgt caggtgaatg atgaaccgag
    15721 tatggcggcc ctggagcaat gtgctcacag cccgcaggtg attgcgctgt taaacgagtt
    15781 acaacatcaa ctaagcgaac gccaaccgcc gttgggcgag gtgctggcag tcgatctgtt
    15841 aaatctcaac gccgacgatc gtcactttat caatacgctt ctcggggaag gggaagtgtc
    15901 agtgcgcatt cagcaggctg acgacagtga aagtgaaata caggaggcga tcttctgcgg
    15961 attatggcgg gtgcgcagac gtcgcggcga aaagttgctg gaggacaaac tggaggctgg
    16021 ctgcgcgccg ctggcgttgt ggcaggcggc aacgcaaaat ctcttgccga cagattcgct
    16081 gttaccgccg cccattgatg gcctgatgaa tggcctaccg ttggcgcatg agttactggc
    16141 acatgtacgt aaccccgacg cgcagccgca cagcattaat ctgacgcaat tacccatcag
    16201 cgaggctgat cggctttttc tctcacgtct ctgtgggccg ggaaatattc agattcgtac
    16261 cattggctat ggcgagagct atatcaacgc cacggggtta cgccatgtct ggcatttacg
    16321 ctgtacggac accttaaaag gcccgttact ggaaagttat gaaatctgcc caataccgga
    16381 agtggtgctg gcagcgccag aagatttggt cgactctgcg cagcggctta gcgaggtatg
    16441 tcagtggctg gcggaagctg caccgacgta aaaagacggt aagtatcgct ttcagtctta
    16501 tgaatatcgc aatcggcgaa tacctctggt cgtagagttt caggataaag agggagatct
    16561 accattatcg ggttattttt ctctcttcgc ctacaggagt gcgcatgtgg gatgtcattg
    16621 atttatcgcg ctggcagttt gctctgaccg cgctgtatca ctttttattt gtacccctta
    16681 ccctggggct gatttttttg ctggctatca cggaaaccat ttacgtggtc accggcaaaa
    16741 caatctaccg cgatatgacg cgcttctggg gtaagctctt cggtatcaat tttgctcttg
    16801 gcgtggctac cggcctgacc atggagtttc agtttggtac taactggtca ttctattcca
    16861 actatgtggg cgatattttt ggcgcaccac tggcgatgga agcattaatg gccttcttcc
    16921 tcgaatccac ctttgtcggg ctgttcttct tcggctggca acggctgaat aaataccagc
    16981 acctgctggt gacgtggctg gtggcgttcg gttcaaatct ctctgcgttg tggatattga
    17041 atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt tgatatcgac accctgcgta
    17101 tggagatgac cagcttcagc gagctggtct ttaacccggt cagccaggtg aaatttgtgc
    17161 acaccgtaat ggcgggctac gtgaccgggg ccatgtttat tatggcgatc agcgcctggt
    17221 atttactgcg cggacgggag cgcgatgtcg cattacgttc gtttgccatc ggttccgtct
    17281 tcggtactct ggcgattatc ggtaccctgc aactcggaga cagttctgcg tatgaagtcg
    17341 cgcaagtaca accggtaaaa ctggcggcga tggaagggga gtggcaaacg gaacctgcac
    17401 ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca agagcgtaac gcctttgcca
    17461 tcaaaattcc cgcgctgcta gggatcctcg ccactcactc attagataaa cccgtgccgg
    17521 gtctgaagaa tttgatggct gaaacctacc cacgcttgca acgcggacgt atggcctggc
    17581 tgttaatgca ggaaatatcg caaggcaatc gtgagccgca tgtgttgcag gcattccggg
    17641 aactggaagg tgacctgggc tacggcatgt tgctctcccg ctatgcgccg gatatgaatc
    17701 atgtcacagc cgcacagtac caggcggcga tgcgtggcgc gatacctcag gttgcgccgg
    17761 tattctggag tttccgcatc atggtgggct gtggttccct gctgctactg gtgatgctga
    17821 ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca tcgctgggtg ctgaaaatgg
    17881 cgctctggag tttgccgttg ccgtggattg cgattgaagc cgggtggttt atgaccgagt
    17941 ttggtcgtca gccgtgggcg atacaggaca tcttaccgac atactccgcg cactccgctt
    18001 taaccacagg acaactggct ttctcactga tcatgatcgt agggctttac accctgttct
    18061 taatcgccga agtctacctg atgcagaaat atgcccgtct ggggccgagc gcgatgcaga
    18121 gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc atgtttgatt atgaaacatt
    18181 gcgcttcatc tggtggctgc tgattggcgt gatcctggtg gtctttatga tctccgacgg
    18241 atttgacatg gggatcggct gtctgctgcc gctggtggcg cgtaatgatg atgaacgccg
    18301 gatagtgata aacagcgttg gtgcacactg ggaaggcaac caggtctggt tgatcctcgc
    18361 tggtggggca ttatttgccg cctggcccag agtgtatgca gcggcgtttt ccggctttta
    18421 tgtggcgatg atcctggtgc tgtgctcact gttcttccgc ccgctggcct ttgattatcg
    18481 cggaaaaatc gccgatgcac gctggcgtaa aatgtgggac gccggtctgg tcatcggcag
    18541 tctggtgccg ccggtagtct tcggtatcgc cttcggcaac ttgttgctcg gcgtgccgtt
    18601 tgccttcaca ccgcaattac gcgtggagta tcttggcagc ttctggcaac tgctgacgcc
    18661 attcccttta ttgtgcggat tgctcagcct tgggatggtg attttgcaag gtggcgtctg
    18721 gttacaactg aaaactgttg gtgtgattca tctgcgttca cagctggcca ccaaacgcgc
    18781 tgcactgttg gtgatgctgt gctttttgct ggcgggttac tggctgtggg tcggtattga
    18841 tggctttgta ctgctcgccc aggatgctaa cggtccttcc aatccgttaa tgaaactggt
    18901 ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa tcgcccgttt tgtggatctt
    18961 cccgctgctg gggttcttct gcccattgct gacggtgatg gcgatttatc gtggtcgccc
    19021 gggttgggga tttttaatgg cgtcattgat gcaatttggc gtgattttca cggcaggcat
    19081 cacgctgttc ccctttgtca tgccgtcaag cgtgagtcca atctccagcc tgacgttgtg
    19141 ggacagcact tccagtcagc tgacgctgag cattatgttg gtaatcgtgc tgatattttt
    19201 gcccattgtg ttgctctaca ctctctggag ctactacaaa atgtggggac gcatgacaac
    19261 agaaactctc cgccgtaacg aaaacgagtt gtactaagga gcaggaacaa tgtggtattt
    19321 actttggttc gtcggcattt tgttgatgtg ttcgctctcc acccttgtgt tggtatggct
    19381 ggacccgcgt ctgaaaagtt aacgaacgta ggcctgatgc ggcgcattag catcgcatca
    19441 ggcaatcaat aatgtcagat atgaaaagcg gaaacatatc gatgaaagcg atcttaatcc
    19501 catttttatc tcttctgatt ccgttaaccc cgcaatctgc attcgctcag agtgagccgg
    19561 agctgaagct ggaaagtgtg gtgattgtca gtcgtcatgg tgtgcgtgct ccaaccaagg
    19621 ccacgcaact gatgcaggat gtcaccccag acgcatggcc aacctggccg gtaaaactgg
    19681 gttggctgac accgcgcggt ggtgagctaa tcgcctatct cggacattac caacgccagc
    19741 gtctggtagc cgacggattg cgggcgaaaa agggctgccc gcagtctggt caggtcgcga
    19801 ttattgctga tgtcgacgag cgtacccgta aaacaggcga agccttcgcc gccgggctgg
    19861 cacctgactg tgcaataacc gtacataccc aggcagatac gtccagtccc gatccgttat
    19921 ttaatcctct aaaaactggc gtttgccaac tggataacgc gaacgtgact gacgcgatcc
    19981 tcagcagggc aggagggtca attgctgact ttaccgggca tcggcaaacg gcgtttcgcg
    20041 aactggaacg ggtgcttaat tttccgcaat caaacttgtg ccttaaacgt gagaaacagg
    20101 acgaaagctg ttcattaacg caggcattac catcggaact caaggtgagc gccgacaatg
    20161 tctcattaac cggtgcggta agcctcgcat caatgctgac ggagatattt ctcctgcaac
    20221 aagcacaggg aatgccggag ccggggtggg gaaggatcac cgattcacac cagtggaaca
    20281 ccttgctaag tttgcataac gcgcaatttt atttgctaca acgcacgcca gaggttgccc
    20341 gcagccgcgc caccccgtta ttggatttga tcatggcagc gttgacgccc catccaccgc
    20401 aaaaacaggc agatggtgtg acattaccca cttcagtgct gtttatcgcc ggacacgata
    20461 ctaatctggc aaatctcggc ggcgcactgg agctcaactg gacgcttccc ggtcagccgg
    20521 ataacacgcc gccaggtggt gaactggtgt ttgaacgctg gcgtcggcta agcgataaca
    20581 gccagtggat tcaggtttcg ctggtcttcc agactttaca gcagatgcgt gataaaacgc
    20641 cgctgtcatt aaatacgccg cccggagagg tgaaactgac cctggcagga tgtgaagagc
    20701 gaaatgcgca gggcatgtgt tcgttggcag gttttacgca aatcgtgaat gaagcacgca
    20761 taccggcgtg cagtttgtaa tgcataaaaa agagcattca gttacctgaa tgctctgagg
    20821 ctgatgacaa acgaagaact gtctaatgcg tagaccggaa aaggcgttca cgccgcatcc
    20881 ggccactttc agttttactc tttctcggag taactataac cgtaatagtt atagccgtaa
    20941 ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga tagcgccttt aatattgacg
    21001 cctgcctgtt ccagacgctg cattgacaaa ctcacctctt tggcggtgtt caagccaaaa
    21061 cgcgcaacca gcaggctggt gccaacagaa cgccccacga ccgcggcatc actcaccgcc
    21121 agcatcggcg gcgtatcgac aatcaccaga tcgtaatggt cgttcgccca ttccagtaat
    21181 tgacgcatcc gatcgcgcat cagcagttca gacgggttag gtggcacctg accgcgagta
    21241 atcacatcaa agcctccttt gccaaaatgc tggatcactt tgttgagctc atctttacct
    21301 gccagatatt ccgacaagcc atgttcatta ctcacggtaa acaggttatg cgaataacca
    21361 cggcgtaagt cggcatcaat aaataacact ttttgatcgg actgggcgat caccgctgcc
    21421 agagttgaac tgacaaacgt tttaccactg tctggcgtcg caccggtgat catcagaatg
    21481 ttattctccg tctccatcat agcgaaatgc agactggttc gtagcgcacg tacggcttcc
    21541 acagcagaat ccgccgggtt atccaccgcc aggaagggga tatttttagt acgatggcgc
    21601 tgctgattag aaaataaatt tttcttacgc agacgggtgc gtttatccag ccactcggac
    21661 attgggatag tggcataaac gctgatgccg tgctcttcca gttgttccgg ggcttctaca
    21721 ccacgacgca acatcgcacg cgccagcacg gcacccacag aaataaacag gccaagaata
    21781 aaaccaagca ccacattcaa cgcttttttc ggtttcactg gctgcggctg agtgactgcc
    21841 gggtcgataa tccgcacgtt accaatggca ctggatttcg aaatactcaa ctcctgctgg
    21901 cggttaagta attgcagata taccgcacgg cccgcttcta cgtcacgact taaacgcaac
    21961 acttcctgtt gggtggaagg cattgccgat acccgcttat tcaggcgttt gcgttcttgc
    22021 tccagcgtct ggcgtttttc cagcagcgca cgataagttg ggtgatcttt cttatacagc
    22081 tgggagatct ctgcctcgcg gaaggtcagc tcattgagtt gattatcaac gttcacaatc
    22141 tgctcaagaa cggctttggc ttccaggtta aggtcaaccg aatcgcgctg ctggcgataa
    22201 acgttgagtt tttcttccgc ctggtccagc tcgctgcgca cttcaggtaa ctggcgctgt
    22261 aagaattcaa ggctttgtga atcctgcgcc gcctggcgag cgatattctg ttgcaaatag
    22321 ttgttagcga tgctgttcag aatacgagta atcaactggg gatcatcacc agtcatggta
    22381 agttccagca tcccgctttc tttactgcgt tcgctaacgg taaaggtttc ctgcaatgcg
    22441 ttaatcgctt ccagttcggt acgctggctc aggacaaact gtgttcctgg tttggcctta
    22501 atgtccgcga tagtcagcgc aacgccatct ttttccagac gctggccgac cataccattg
    22561 acggtgaact cttcaccttc cagtgtatag tggccgtttt ccccaaccgt gagtgtcagt
    22621 tgctgatcct gaccattcag ttgtggaata tgcatccagc tgatcgccag ctcacctggt
    22681 ttttctttgg ttaatctcgc ccagccgcga cccacaatcg gaaaatactt ctgctcaact
    22741 atgtcgcgca gattcagttc agcaatggtt ttaccgagaa tcatgcgcga ttgcagcagt
    22801 tggatctccg gtgcagactc gggcgatgag ttagggatca tatcgctcag gccgctgaga
    22861 atggcgttgc cctgtttttg ctcaacctgg accagagtat ctgcctgata aattggtgta
    22921 cttaacagcg agtaagcgac agcgatcagc gtgaataacg cggtcacgct gataataaac
    22981 ttacggtgat cccataactc gccaaccaga cgaagcagat cgatctcatt ttcctgagtg
    23041 ctgcctggtg gcgtattcat atttttagtt gtcatcgtat tttataaact caaattaccg
    23101 gctgaggcgc ttcgcccatt cctgactggc gcgctccaac ataccgtaga catgttcaaa
    23161 tgcgtcctga cttttacgat agggatccgg gatctctttc tgttccagcc attgcccaaa
    23221 cagcattgtc ttcccgcgga cctcgggagc gattgcggta acctgagcaa tatgctctga
    23281 ctccattgcc agaatcagat cgtaatttcg tgccatctca gcagtgagct tacgtccggc
    23341 atgtccttca agagatacgc catgattagc ggcaacatct gctgctgtcg catctgcagg
    23401 gtgttttacc agaccatgaa cacctgccga tttgactttt acgcctggca gacgtttacg
    23461 cagcaagcgc tcgccaatgg gcgaacggca aatgttgccg gtacaaacca ccaggattga
    23521 gttaaatttt agttgggcca tgtttgaatc caccgtgaag tctctgtcag gtcatgcacc
    23581 ccggaaatcg tcggtaccag cagagaaatc acacggttcc aacgagccag cggcgcagta
    23641 gtgacataaa caatgtcgta aggttcaagc tggaattccg ttccgagtat cattgccgat
    23701 gcgtccttcg cattcagctg ataaatattg gcgattttgc cattctgctt gttttgcgtt
    23761 gcacgaataa caaaaatccc cgtcgcatca gcgacatcct gattcatacc ctcagcgtta
    23821 cccagcgctt ccgccagggt cataccgctg cggtccattt tcagcgtgct ctgtttaccc
    23881 acttcaccca tcacgaagac tttcagatca tcgttgcgcg gaataaataa aatatcgccc
    23941 gggtgcagca gtttgttctg gcgcaagtcg ccacgctgca tcagcgcata gaggttcact
    24001 ttggtcttta cgccattctg ggtgagcacc acgttgcgcc agtctgcatc tgcggtcaga
    24061 ccgcccgcgg cgttaatcgc atccatgatg gtcagaggaa tattggtaat cggctgctga
    24121 cccgatttgc tgacttcgcc ggtgacgtac gctttttgcg agcggaacgc cgcgacgctg
    24181 acatcgactt gcgggctttc aatgactgaa tccagacgcg ctgtaatctc gttgcgtacc
    24241 tgggtaagtg ttttcccggc cactttcagg cgaccaatat aggggtagaa aatcgcacca
    24301 tcggcgttaa cccagttgcc ggtatcactg gcgctacggt attgccctgc gggggtggtt
    24361 agttccgggt gatcccagac cgtaaccatc aacacatccc caatgccgat acgatattca
    24421 tagtttgcga tttgctgctc cagctccggg ttagcctgcg acataatcgg tttggcgcgc
    24481 aactgatcaa tcaatcctgg cgtgacggga tagatattga ccattttgtc caggtcatac
    24541 tgattgtcag gtagttcaat gacatccttg ttactggtcg ataaattctg accaggaaca
    24601 agggtacatg ctgtcaaaga cagaacagcc aatgttagta ccgaaaactt aaaaatgttc
    24661 tttttcattg gtttatattt atcagtcaat gtaatgttat gaattacgtc aggttattgt
    24721 ccaaccggta cttctcgctc tgccgtcatt ggatagagct ggtatttgcg tgacagcatt
    24781 tgtccaccat cacgcgtcaa tggtgtccac gaaaccaccg cgcggttgcg gttaggtcca
    24841 atggtcatca agtcgaatgg aatcgagata taaaaacctt tactaaagcc gccttcgccg
    24901 tagtcatctt tagatacgtt actgattgcc gcccataccc ctaccgccac gccgctgtca
    24961 aagcgtttgg cgacgtcgat cgttgcgcct ttatcttttg ccagatattg cccaacgcta
    25021 agtttcatca gtacgccatt gagcgtcggc gggttccagt aagccgtcac gaaacctgtt
    25081 ggcgtggaat aatcggtgaa acgcatcatg ttgtcccagt cacgctgctt cacgtagtta
    25141 acgtccacac ccagcgccca gctggcatct agcgggcgat aaagcagctc ggaaccgaca
    25201 ccggcataca tcgtttccag ataaccgcca tacacctggc catagaaacc attgcctaag
    25261 tcagcaaaat agttcgcctg caagttgttg agataaacgt cattgcgaac gtagtcacgg
    25321 atatgcgtgc gcacgcgggg cagggtagag tccgcgggca acagcgaaga cttaaacttg
    25381 tcgtagttgt tgtaaatatt ggtgaaaata ccgccatcaa gcagcaggtg gtcggtaaac
    25441 cagtagcggg cactggacat cagccccagc tggaacatat agaaatcttc cgggccgccc
    25501 agcgactgtg aaagtgttgg gttgaaagag tagctaaagc gatcttcacg aatacggtag
    25561 ccccgaccaa aggcagaaag atcttctgct tcaacacgtt gttgttgaag ttgctctgat
    25621 tgaccaggcg ctgtaccagc cagctgtttg cgcaggctgg ctacgtcggt ttcggtagtc
    25681 accatcgcca tatgctcgcg cttttgcgtc acgctaatct tctcaacgcc ttgcggcagg
    25741 ttattcacca gaatccggtt ggcacgatcg accgcttcgc gagaatcacg gtatttgtat
    25801 tgttgaccag acatatacag cgtcttatcg cgcagctgaa tttccggtgc ttcaaaacct
    25861 gcgttgtact tcagcgcggt aagttggttc gctactgtgg tgtactgcaa tccttcagat
    25921 tcaggcgcag gttgatatgc cggttttggc gtatcgcgca gggcagggcg cagatcgttg
    25981 aaattggtcc gtaacgtgaa gccaaacatc aacgtgttac cgcgttcata actcaggttg
    26041 agatctgccc agctggcagc gcgataaact gcgccgacgt tgaaatggct tgcctgaggc
    26101 agtttgccag cgaaatcatt ctggtaattg ttgccatcgt attcgagttt cagacgcagg
    26161 ggattccacg gcgtttgata ctcaatgccg ccaaagatgg aagccgggcc acgaaagata
    26221 tcgctaaagc tgatatcgcc cgcatcgtga gactctgcgc gatgacaata tttatcgctc
    26281 acccggcaaa acgggttggt aatattgccc gcattaccgg cgtagcccca tgccatcccg
    26341 agggtgaaat caaatggccc cgcttgcttg ctggccacca gatactcacc gtcaaacaga
    26401 cccgtaccag caatatcacg tttaccaaac gccacttgcg gtagccagta accttcttcc
    26461 cacaggcgaa gtttaaaatc gaatgatttg tctttgtatg actgatcgcc gctgaaatct
    26521 tcccactggc tatatttgcg tgtgcgcaca tccgtataac gaatggtgcc ttccagccat
    26581 gggaacagcg ccacggaggt ggagtagaac cggtattgat cgttatcccg gtagttgacg
    26641 ctgaattcac cttccggtgc gatgcgcgca tttggcatct gcaacaatcc tgtgccgcca
    26701 aagtctgatt gcgacgaacc cagcggatcc gggtaggtta atacttcagc atgacaggca
    26761 ctggagacgg caatggccag gcagcttaaa agataagaat tcttcttcat cagtcaggaa
    26821 cacgttgcgt taaaacagac acaatctggt catttaaatc agcatatttt tcgggcagta
    26881 catgcgcaga aaagcccaac caaagctggc tgccaggcgg tggttccaca tgtcttttat
    26941 tccacagggc aacaggggcg acgacagtct caccctcagg cgtaatcacc ataacgttat
    27001 tcttgtccgc gcctgcaagg cgggggtgat cttgcagata atcggtaacg ctacggcctg
    27061 cctgccaggg caattgccca gccccagaaa ccgcacccag cagagtaata gtcaccggac
    27121 gctgtacggt atagagcgta tagtccccga ccaacggggg attgctgttc tcatccactc
    27181 gaacaaaatc agggtccagt ttgaccggta agcgaccagt gatattgaga ttgagtaact
    27241 gctggcgcac ggatttaatc gttgccgcca catcgtcgtc agcttctgct tcccaggaag
    27301 caagctgtgc catgacatgt tggtagtctt taagcgcttt agctttcgcc gcagagtcgg
    27361 tcagcaaggc accaggccac cagaggcgat cgcgcagttg cggttgtgtc accaactgga
    27421 caacattttc cacggggcca acggaaagtg tctgttgttc gccaggcaga taaatagtca
    27481 ccgttccttg cgcaaaggca tggggtgtca ttacgtaaag tacgctggca atgaaatacg
    27541 actgtaattt attcatggtg cgggtttaag aaacgtcatt tctacgggaa taacccctgc
    27601 gcctaacatt tgccgacttt gacgaacctg gccagtagcc gaatcgaccc agaatgtgtt
    27661 ttgccactgt ttttctggcc gcgtggaagt cacctcttcc tgccagacat tacacagcac
    27721 attgcgaccc gcaatattca gcgtctctaa tccagcaaag gaaaaagtag aactcagggt
    27781 cgcagagcga aaatggttgt cttcactcca gagaatatcg cgcgtccagc ggctgccatc
    27841 ttttattgcc agcgcgttac gcagagggtc ctgcccggaa tttgtcactt ccagcagatt
    27901 attattaagt ttgacggttt ttaaaagacg tccattgtgg gtaaccagca tggcgttatc
    27961 ctgggataac catttgcttt gttcttgttc tatatatccc agtaccacaa agattcgttg
    28021 cccttcatta aggcgtaaat acatcgtgga ataaggcaac gcctggatct gctgatccgc
    28081 tacggtgata tcctgattat cgaaaaggct ggcgcgaaat gtatcgacca tactttgctg
    28141 gctgtgcgta catcccgcaa gaaatagtgc gaaaatcgat aaaataagag ggcgcacctt
    28201 taatccttga aaggttaatt aaaaaaatgc aaatctctaa agcccggtac gaatatcgca
    28261 accgggtatc atttaacttt aatttcgacg cgttaaagca gtattagcgt gtagtggacg
    28321 tggtggttga ggtgttatga gaaccgccat cgccactgtc atttgctgca acaactgcca
    28381 acgtcgccac accaacagcc gttgccacac cgacaactgt cgccgtgccg acagcgccag
    28441 cagccacgcc accagtggta gtagcagtcg tggttgcgcc ggtagtggtc gttgtggtgc
    28501 cagtagtacc cgtcgttgcc tcggtcccgt ttgttgcttc aggggcagca aacgcagctg
    28561 gcgtcgtcaa catgaaggcc ataaggattg cagaaagctt gtgtttcata acttttcctt
    28621 tattcatcgc atggacaata cgtacgcttg agatcaggat tgtctacttt gagtataaca
    28681 tccttgtgaa atctcccttt cctcataccg cttgcactat atactgcggg taggaaaggc
    28741 gcgcagaggg aaatataaga ttgttaacta tccccctctg gaaatacgac ttgtgagcat
    28801 aagataaatc ttagcaaaat acaaattaaa gataaggaaa ttgttgcgaa agctattaat
    28861 tattgttggt aatatagttt caagtggtac tataactggc tgttaaatgg catttataac
    28921 tattaggtgc agagatattc gcttaaatgg attagtttct ttgtggaaaa gtaactgatg
    28981 ttataatgta tgatgttgac tattgcacta ttccaataaa accagtcagc tttaaacaag
    29041 cagcgtcata ttaagagaga taaacatttg ccgctgttgg tcctcgcagg ccatttacgc
    29101 ggcaaaattc cacacgtaat cctggtataa gcacttctgc gtcgcgggga gtgaatgcgg
    29161 aaatatggac ctgaacttct ttacgaccgt cggaggggat aatgaatcct ttgccgcttt
    29221 tgcgatcaaa ggttttgaca attcctgtca ttttacggga caaacaaatt ccttactgaa
    29281 aatactgcgc tgcactatac ggggttaata aaataaagcc agcgatattt aagaccgccg
    29341 gacggctaaa ataaaatttg cttaatctca attatcatgc gttaatagct gcgtcggttt
    29401 gaaagacaga cagcatacaa agtagtttac taaagcagtt ctcattatca ggcattatcc
    29461 ccttcttttg agtctctctc ctgaacacta agtagtttct gtattaaagc cctgtttgcc
    29521 gaaaggccca aaatgaagga agtaaaatat gtctaataaa atgactggtt tagtaaaatg
    29581 gtttaacgca gataaaggtt ttggctttat cactcctgat gatggcagca aagacgtttt
    29641 cgtccatttc accgccatcc agagcaatga attccgcacg ctgaacgaaa atcagaaagt
    29701 tgaattttct attgagcagg ggcaacgtgg ccccgcggca gcgaacgttg ttacgctcta
    29761 aggttgccgt tattactcaa catctccatt tccgctgtcc atgttgtcat ggttcacagt
    29821 accgcacatc ggcattcgat gtgacggagc gaaacccttt gggcgctaag tgtatttttt
    29881 gtaaatcgac gatgatcacc tttgataacg tcgcgctgca aatatgcacg gaccatgcgc
    29941 cgctggattt cacaaaataa tatcaggctc cctcgtggag ccttttttat atctgcctta
    30001 tttttcttca acgctgtatg tatagtaagc gataacctgt tgattattga atctttcggg
    30061 gagatggctt ataacatttc ttacctgacc agggtaccgg gaaccaacac cttactggcg
    30121 tgttgctgtc tttcaagacc agaagaggtt aacagtgaat attgaagagt taaaaaaaca
    30181 agccgaaacg gaaatcgccg actttatcgc gcaaaaaatc gccgagctga acaagaatac
    30241 agggaaagaa gtctctgaaa ttcgcttcac cgcacgagaa aaaatgaccg ggcttgaaag
    30301 ttatgatgtc aaaatcaaaa taatgtgatt ttgtgaacat cacccgtgcg aggtgatgtt
    30361 ccgcttgttg ctaatttagt gaccaatcat tggcgcttgt ggaattaagc gtcggtacaa
    30421 ttcatccggt accgggcttt gccatactcc cgcatacatt gcgtaaccaa tcaccgcaaa
    30481 cataatcccc agaaccagta gcgtcattaa ccagccagac aacgcaaagg cttttttatt
    30541 tgccgcaggt ttttgcagtg aaaaggtcaa tgttgaggct accggacatg actctacgca
    30601 agtcatacag ccggtacatt ccactgttcg tacctgaatt aatttatcga ccgggatccg
    30661 tgatgggcaa ttttttgcgc atttgccaca gtcgatacaa ctttcggcat tgcgacgaat
    30721 cttaaacggc gacaatagcg aaaccacgcc catcagcgcg ccatatgggc aaagataacg
    30781 acaccaggca tggcgaataa acaggctggc aatcagcaaa acggtcacgc tgattaatgt
    30841 cgcggtcccc atatgacgaa agaaatcgag cattttaacg tccatcacca cgctgtaggg
    30901 cgacaacata aaatagtgaa tcgcctgagc gggcatcaat aacgcgatat agagaaaaaa
    30961 actcaacagc aaatacttca cgccgcgcag aggaatatcc agccagcggg gaaggacaca
    31021 ttgccgacca aacagtttgt taccgagatc gccgattaat tcagaaagcg taccaaccgg
    31081 gcataaccat gagcaaaagg cctttttgag taatagactg atgacgataa aagcgaccaa
    31141 taacagcatc gcggcggcgt ggacggacgg taactgacct gttacaaggc tatatttcag
    31201 attcatcagc ccggcaatcg gtagccagcc ttcgatacct cccggtctgg cgacaaatgt
    31261 cgtgctactt gccgtttcgt aatagcgcac ccaataccag aacgtgatgg caatataaat
    31321 attcgttgcc aacagtaata attgcgtcgc tttacgccag gtcgtggcat tacgccagtc
    31381 attccacggt aatttgccgc ccgtcgtgcc tggccgccgc tgccagcggg ttcttttatt
    31441 ctctgccatg attttgccag tccgttaagt tgtataccaa atgccactat tctagttgtt
    31501 cttaactggc tgatattgat tcaaatcgcg ttcaggtctt tcttatgcaa ccatgcttcc
    31561 agagcggcaa cactgcgtgt aatttcttcg tgtggaaggg gggcagataa tggctgctgc
    31621 tccagttgtg cgcatagctg gctggcgata cgcattccca gactcgagca actgcttttt
    31681 agctgatgcg cggcacgctt tattttctcg ctatcctgac tggcgcgggc aatgtcgatt
    31741 tcatcgagaa gcggcagggc atgttgtgta aataatacca gccattcgtg gatcttctcc
    31801 gtccccatta actgagcatc ttcattgagt tgcgatacat ccagcgattg atcgttattg
    31861 acttgcagtt ggagatagtg cgccagtaac tgaccgagca cttcacgcgg caccggttta
    31921 gggataatcc cgcggaataa tgaactggta cgctggcgca gcgtttcgtc aatgacatgg
    31981 gcgctaaagc caatcaaaac cagcgacgga tattgctgtg ccagttgtcg ggcaagcgta
    32041 atgccgtcga catccggcag atcaaaatcc accagtgcgg cagcaaacgg ttcgctattt
    32101 tgcagtgtct ctaaagcctg cgcggcattg ccaacagcaa caacctgcgc accactggtg
    32161 ttcagcatct caacggtaat tcgctgggtt agcgggttat cttcaattaa cagcaaacgt
    32221 aaaccgtcaa gacgcaccgc ctgattgact gtttttggca cgggtgccgt ggcaacacgt
    32281 aacggcaagc gtaaacaaaa acagcttcca acctccggcg tgctggtggc gctcagttcg
    32341 ccgcccatcg cctgggccag acggctactg atagtcagcc ccagcccggt gccgccgcgt
    32401 ttgccgctta cctgcacaaa tggctggaag atttctgcca gtttcgcggg atcaataccg
    32461 cagccgctgt cttccacttc gaccagccat tgctcaccat cagtgcgact acgcaggata
    32521 atgtaccctt cgtcagtaaa acgcagggcg ttgctcaaca ggttggttat aacctgacga
    32581 atacgtcgtg gatcgcccat taacgcgcac ggcatatcat cggcaattgc cgttgccagg
    32641 cgaatcgggc gacctttcac ccgtccgctc attaattgca gggtactttc cagcagcggg
    32701 cgcggttcaa agggctcatc gctgaccgaa acattcttgc cacctgcttc gatagcggaa
    32761 taatcgagaa tatcgttgag gatggtcagc aacgattcac cagagtcagt aattgcccgc
    32821 aaatcatcac gctgggcgtt aagtgcgggg ttatctgcca gcagttgagc agtgccgaga
    32881 ataccgtaca gcggtgtgcg gatctcatgg ctcatcgccg ccagaaacgc cgattttgcc
    32941 tggctggctt tttctgcttc cgcccgtgcc tgtcggtgtt ctatcaccag ttcctgcaat
    33001 tcagctgtac gcgctttgac ctgcgccgcc agctgttcgc ggtggcgatt cagtgcatga
    33061 acattgctgc gaaacgcatc catcagccgc ccgatggtat ccagctcccg tacgccagcg
    33121 gtttccggga aaggggagtc aatatcaccg tccagcagcc gttgcagcgc ctgcgtttgt
    33181 tcggcaagtg gacgcgtgac tgagcgataa accacgcgcc aaaggatcag aatcagtgcg
    33241 caaagtgaaa ccatccccag caataacagg ctgtattgcc cgcgtgcact ggctttttcc
    33301 agatgtgcca gtccatgctg attgcgcagc tcaatggtgt cgaccagctg actgacttcg
    33361 ctactaaact gcgcgaactg ggcgatatta ttttgggcga gagtttgcag gtgattgctg
    33421 atttcactgt cctgctggaa cagcgccaac agatcgctat attggctaac ggtagttaac
    33481 gttgttgcga cctgcgcacg aacacccgga tcttcaatgc gtatttgccg acgttgcaga
    33541 attttcaccg cattattgag ctgcttttcc agcgttggcg catttttctg gatctgctcc
    33601 agccccagat tcatcaccat ttgctgcacc cgcagggcgc taaggcgcag ttcattcatc
    33661 tggttaacat actcaagatc gatatcaatc agccgatcga gtgcactttc tgcggcctga
    33721 cgctgatctt gttcgatcaa atcgtaaatc ccggcctggg tcgctccggc ggaggttgcc
    33781 gcattattcg cctgaccttg cgccaggcgt gcgatctcat cggcggcagc gactatctgc
    33841 tgactgagtt gctgttgttg ctggcgcagt tgcaaacgct gccccaccag ttccccttgc
    33901 tgacgtaacg aacgggagat ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct
    33961 tgttcccgta acgcttgcag caacgcatta atcttcaggc tttgtgcggt gagcatttgc
    34021 ccctgcgcct gccacatctt ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt
    34081 tcccaggcgc tggcttcgct caactggcgc gccatattca tggtaggaat caatgcctga
    34141 gtattgtctt tttccacctg gctgataaag cgcaggttgt accatcccac cagggtactg
    34201 gtcagggtta acagcgccat cagggcaaag cccatccaga gtcttcgggt cagggttaaa
    34261 ttcacggtcg gtgcacttta ggtgaaaaag gttgagtcgc aaagcggaat gcatctagca
    34321 taaagcctta ttattgatga ggctatcatg cgcgtactgc tatttttact tctttccctt
    34381 ttcatgttgc cggcattttc ggctgataac ctgttgcgct ggcatgatgc gcagcatttc
    34441 acggtgcaag cctctacgcc gcttaaagcc aaacgcgcat ggaaactgtg cgcgctttat
    34501 cccagcctga aagattcata ttggttatcg ttgaactatg gtatgcagga ggctgctcgc
    34561 cgctacggtg tggatttaaa agtgctggag gcaggcggct acagccagtt ggctacccag
    34621 caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg ccattttgct cggtagtagc
    34681 acgacctcat ttcccgacct gcaaaagcag gtagcaagtc tgccggtgat cgaactggta
    34741 aatgctattg atgctcccca ggtgaaaagc cgcgttggtg tgccctggtt tcagatgggc
    34801 tatcaaccgg ggcgatatct ggtgcaatgg gcgcacggta aaccactgaa tgtgctgttg
    34861 atgcccggac ccgataacgc cgggggcagt aaggagatgg tcgagggttt tcgcgcagcc
    34921 attgccggaa gcccggtgcg tattgttgat attgcgcttg gtgataacga tattgaaatc
    34981 cagcgtaacc tgttgcagga gatgctggaa cgccacccag aaatcgacgt cgttgccgga
    35041 acggccattg cggcagaggc ggcaatgggt gaagggcgta acctgaaaac gccgcttacc
    35101 gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt
    35161 atggctgcca gcgatcaaat ggtctggcag ggggaactgg cagttgagca ggccatcagg
    35221 caattacagg ggcaatcggt gtctgataat gtcagcccac cgattttagt tctgacgccg
    35281 aaaaatgccg accgagaaca tatccgccgc tcgctgtcac cagggggatt tcgtccggtc
    35341 tatttttatc agcacacatc agcggctaag aaataacctt caccatgttg cgtcaccagt
    35401 aaatccgcgc tgagtttatg acgtaaacga cgaattaaca catcgacggt gcgcaggtca
    35461 gggttttcca cccgacgcgc agaaagcata cgtagcagac gttcacggct gagaatttcg
    35521 cccggattcg tcacaaatgc caccaacatt tcatactctg cgcgggtcag tttaatcggc
    35581 tcgccatccc gctccagcgt atggcgcgac acattcaggc aataaccggc aaagcgatag
    35641 cagttgtcct gagtgtgcgg ttgagcttgt cgcgcgaggt cgattcgcca gagcagattt
    35701 ttcacccgta ctaccagttc gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc
    35761 atttccagcc caacaatacg gtcaatccga tcgctgcgtc cggtaaccag aataatcccc
    35821 accgttgagc gttctcgcag ggcgcgggtt aacatcaggc cattttcatc gggtaagttg
    35881 atatccagca gaattaaatc taccggctga ttctgcataa tttcccgcag cccggcaccg
    35941 ctcgctgtaa cggaaacggt atacccctcc tgagtgaagt aagattgtaa tcgcgcctgg
    36001 gtaaccggct catcttcaac aataacaatg tgatgtggca tcagagggtt ttactcattc
    36061 tgttcatatc tgttcatatt ctgccgtaag ccgttcatcc tgaccagtgc cgctgttcat
    36121 atttgctcat taagatcgct tcactaaacc ataattttac aggggctatt atgcggaaac
    36181 tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg gtcgcaattg
    36241 ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa gtcaccagca
    36301 caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa tataaacagt
    36361 cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt catatcccgc
    36421 cggatattcc aggcatggtg aagcgcaaac tggaagcgag caatgatatc taccagacct
    36481 ttattgctca ctccattgat acacctgaaa aattcgaagc caaacgcgcg gaacttgccg
    36541 agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac ctgccgctcc tgccataact
    36601 acgatgcgat ggatcatgcg aagcagcatc ctgaagcggc acgtcagatg aaggtggcag
    36661 cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag ttaccggata
    36721 tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc cagtgctaat gacagtggtg
    36781 acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat aaagaagcct
    36841 ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc gactggctgc
    36901 aaattgaaat taccggctgg acggaaagcg ccggacgtca gcgtgtactc acccaattcc
    36961 caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag gtaaaaacgc
    37021 tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag gccactgcgt
    37081 ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcttat gcggattcgt
    37141 tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac tttgacgcta
    37201 acggttggat cggcacgctc aacggcatga ttggctttac cagtctcgat aaacgtgaag
    37261 aacgcacctt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt aaggctcacg
    37321 gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat cacgtcggcg
    37381 ttttctggca caactcggcg gcttaaccgt cgccgggatg ctggggccgt cattgttaac
    37441 gccgcgccgt gcgactgcgg cgcaagcggc gactgacgct gtcatctcga aagagggcat
    37501 tcttaccggg tcgcactggg gggctatccg cgcgacggtg aaggatggtc gctttgtggc
    37561 ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg attgccggat tgccggatca
    37621 cgtacacaac gcggcgcgta ttcgttatcc gatggtacgc gtggactggc tgcgtaagcg
    37681 ccatctcagc gatacctccc agcgcggtga taaccgtttt gtgcgcgtga gctgggatga
    37741 agccctcgac atgttctatg aagaactgga acgcgtgcag aaaactcacg ggccgagtgc
    37801 cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt cggggatgct
    37861 ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt actctaccgg
    37921 tgctgcgcag gtgatcctgc cgcgcgtggt tggctcaatg gaagtgtatg aacagcaaac
    37981 ctcctggccg ctggtattgc agaacagcaa aaccattgtg ctgtggggct ctgatttgct
    38041 gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat attacgcgca
    38101 gctaaaagcg aaagtcgccg ccggtgaaat tgaggtcatc agcatcgatc cggttgtcac
    38161 atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta acccgcaaac
    38221 tgatgtgccg ctgcaactgg cgctggcgta tacgctgtac agtgaaaacc tgtacgacaa
    38281 aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtacc tgctgggtga
    38341 gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga
    38401 aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta ttgctggctg
    38461 gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc
    38521 gatgctgggg caaattggcc tgccaggtgg tggctttggt tttggctggc actataacgg
    38581 cgcaggcacg ccggggcgta aaggcgttat tctgagtggt ttctccggct ctacgtcgat
    38641 tccgcctgtt cacgacaaca gtgattacaa aggctacagc agcaccattc cgattgcccg
    38701 ttttatcgat gcgatcctcg aaccggggaa agtgatcaac tggaacggta aatcggtaaa
    38761 actgccgccg ctgaaaatgt gtatttttgc cggaactaac ccattccatc gccatcagca
    38821 gatcaaccgc attattgaag gctggcgcaa gctggaaacg gttatcgcca tagataacca
    38881 gtggacctca acctgccgct ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg
    38941 taacgatctc gaccagtacg gcaaccactc caaccgtggc attatcgcca tgaaacaggt
    39001 ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc tgtgccgtcg
    39061 ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa atgggctggc tgaaacgcat
    39121 ctggcaggaa ggtgtacagc aaggcaaagg acgcggcgtt catctgccag cgtttgatga
    39181 cttctggaat aacaaagagt acgtcgagtt tgaccatccg cagatgtttg ttcgccacca
    39241 ggcattccgc gaagatccgg atctcgaacc gctgggcacg ccgagtggcc tgattgagat
    39301 ctactcgaaa accatcgccg atatgaacta cgacgattgt caggggcatc cgatgtggtt
    39361 tgagaaaatc gaacgctccc acggtgggcc cggctcgcag aagtatccgt tgcatctgca
    39421 atctgtgcat ccggattttc gacttcactc gcagttatgt gagtcggaaa ctctgcgtca
    39481 gcaatatacg gtagcgggta aagagccagt attcattaac ccgcaggatg ccagcgcgcg
    39541 cggtattcgt aacggtgatg tggtacgcgt ctttaacgct cgcggtcagg tgttggcagg
    39601 ggcagtggtt tctgaccgct atgcacccgg tgtggcacga attcacgaag gggcatggta
    39661 cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa tacggtaacc ccaacgtgtt
    39721 gaccatcgac atcggtacat cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt
    39781 ggaaattgag aagtacaacg gaacagtgga gcaggtgacg gcgtttaacg gccccgtgga
    39841 gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca
    39901 gcacaacaga ttgcctgtat ttacgcctgg ctggcgcagt tgttctcccg tgaactggac
    39961 gatgaacaac tgacgcaaat cgccagtgcg cagatggctg aatggttttc gttgctgaaa
    40021 agcgaaccgc cgctggctgc ggcggtgaat gcgctggaaa accgtattgc cgctctgaca
    40081 gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt tctgatgacc
    40141 gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac aggacgagca agagattaaa
    40201 cgcttgttag ttgaggcagg gatggaaacc agcggcaatt tcaacgaacc ggcagatcat
    40261 ctggcgatct atctcgagtt gttgagtcat ctgcattttt cgctgggaga ggggaccgtt
    40321 cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga cggcgctgtg gcaatggtta
    40381 ccagagtttg ttgcgcgttg ccatcagcat gacagctttg gtttttacgc ggcactaagc
    40441 cagttattgc tggtgttagt ggagtgcgac caccaaaaca gataacgtcg tttgtgcgcc
    40501 tgaaaagacg cgtttagcgt cgcatcaggc attatggggc agttgccgga tgcgtcatca
    40561 catccggcaa acacacatca cggatgagtt acaaaccggg aaagtcgctg gcgcagcagg
    40621 cggttttcct gcttcaggtg cgcaatatca tccattaacg tcagcgccac cgcgatccca
    40681 ggccagtcca gagccagttc atgacgcagg cgtactgcgc gttgcaccac gatggcggca
    40741 tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg gttcaaccac ccccaaaccg
    40801 acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc ggtaatagta
    40861 aaagtcaccg taacattagc cattatgctt tcccccaatc tttacgtgga tcaaaagacg
    40921 actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt ttcggcggca
    40981 tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc agacctttgc
    41041 ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg gatagtcagc aaaatgcttt
    41101 ctttcagtgt tggaacggtg actttagcac ccagcgccgc ttcccacggg ctaaccggca
    41161 ctacaatttc cagatcatgg ccgacaatat cgaacagcgg atgtggcgca atatgaatca
    41221 ccagccacaa atcgccattt ggaccgccgt tttcgcccgg cgtcccctgg cctttcagac
    41281 ggatgcgttg accattgccg acgcccgccg ggatcttgac attcagcgtt ttcggaattt
    41341 cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg gtacgcttat
    41401 gctcagtcag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg tggccgcgtg
    41461 tggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag aagatatcgt
    41521 caaaatcttc ggcgttaaaa ctctgaccgt cgctatgatg gaactgacgg ttaaattgcg
    41581 gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca tcacttaaca
    41641 cttcccaggc ttcagcgacc tctttgaagc gggcttcggc atccggttct ttgctgacat
    41701 caggatggta tttgcgggcc agtcgacgat aggcggtctt gattgtcttg agatcgtccg
    41761 tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta tctcgcgtaa
    41821 atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg gtaatcctca
    41881 aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgcggagagg aggtcgcctc
    41941 aaggtggggt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt tttgatagtt
    42001 gaaccaggca ctttaagttt aactggggcg tcattattta tt
//
LOCUS       DAPHDX010000027        41606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPHDX010000027 DAPHDX010000000
VERSION     DAPHDX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.027"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(136..3285)
                     /locus_tag="RH058_001409"
     CDS             complement(136..3285)
                     /locus_tag="RH058_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV3137014.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3308..4501)
                     /gene="acrA"
                     /locus_tag="RH058_001410"
     CDS             complement(3308..4501)
                     /gene="acrA"
                     /locus_tag="RH058_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV3137015.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4643..5290
                     /gene="acrR"
                     /locus_tag="RH058_001411"
     CDS             4643..5290
                     /gene="acrR"
                     /locus_tag="RH058_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV3137016.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5418..8780
                     /gene="mscK"
                     /locus_tag="RH058_001412"
     CDS             5418..8780
                     /gene="mscK"
                     /locus_tag="RH058_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV3137017.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8992..9153)
                     /gene="rsmS"
                     /locus_tag="RH058_001413"
     CDS             complement(8992..9153)
                     /gene="rsmS"
                     /locus_tag="RH058_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV3137018.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9167..9694)
                     /gene="priC"
                     /locus_tag="RH058_001414"
     CDS             complement(9167..9694)
                     /gene="priC"
                     /locus_tag="RH058_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV3137019.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9764..10141
                     /locus_tag="RH058_001415"
     CDS             9764..10141
                     /locus_tag="RH058_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV3137020.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPRVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10294..10845
                     /gene="apt"
                     /locus_tag="RH058_001416"
     CDS             10294..10845
                     /gene="apt"
                     /locus_tag="RH058_001416"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV3137021.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10974..12905
                     /gene="dnaX"
                     /locus_tag="RH058_001417"
     CDS             10974..12905
                     /gene="dnaX"
                     /locus_tag="RH058_001417"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV3137022.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12238..12302
                     /gene="dnaX"
                     /locus_tag="RH058_001417"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12958..13287
                     /locus_tag="RH058_001418"
     CDS             12958..13287
                     /locus_tag="RH058_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV3137023.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13287..13892
                     /gene="recR"
                     /locus_tag="RH058_001419"
     CDS             13287..13892
                     /gene="recR"
                     /locus_tag="RH058_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV3137024.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14002..15876
                     /gene="htpG"
                     /locus_tag="RH058_001420"
     CDS             14002..15876
                     /gene="htpG"
                     /locus_tag="RH058_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV3137025.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16057..16701
                     /gene="adk"
                     /locus_tag="RH058_001421"
     CDS             16057..16701
                     /gene="adk"
                     /locus_tag="RH058_001421"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV3137026.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDVGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16937..17899
                     /gene="hemH"
                     /locus_tag="RH058_001422"
     CDS             16937..17899
                     /gene="hemH"
                     /locus_tag="RH058_001422"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV3137027.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17896..18855)
                     /gene="aes"
                     /locus_tag="RH058_001423"
     CDS             complement(17896..18855)
                     /gene="aes"
                     /locus_tag="RH058_001423"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV3137028.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            19007..20311
                     /gene="gsk"
                     /locus_tag="RH058_001424"
     CDS             19007..20311
                     /gene="gsk"
                     /locus_tag="RH058_001424"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV3137029.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20444..22120)
                     /locus_tag="RH058_001425"
     CDS             complement(20444..22120)
                     /locus_tag="RH058_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV3137030.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22358..23578)
                     /gene="fsr"
                     /locus_tag="RH058_001426"
     CDS             complement(22358..23578)
                     /gene="fsr"
                     /locus_tag="RH058_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV3137031.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23796..25448
                     /gene="ushA"
                     /locus_tag="RH058_001427"
     CDS             23796..25448
                     /gene="ushA"
                     /locus_tag="RH058_001427"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV3137032.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYHLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25485..25964)
                     /gene="ybaK"
                     /locus_tag="RH058_001428"
     CDS             complement(25485..25964)
                     /gene="ybaK"
                     /locus_tag="RH058_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV3137033.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26168..26962)
                     /locus_tag="RH058_001429"
     CDS             complement(26168..26962)
                     /locus_tag="RH058_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV3137034.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27100..27441
                     /locus_tag="RH058_001430"
     CDS             27100..27441
                     /locus_tag="RH058_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV3137035.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27555..30059)
                     /gene="copA"
                     /locus_tag="RH058_001431"
     CDS             complement(27555..30059)
                     /gene="copA"
                     /locus_tag="RH058_001431"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV3137036.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGKSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30321..31253
                     /gene="glsA"
                     /locus_tag="RH058_001432"
     CDS             30321..31253
                     /gene="glsA"
                     /locus_tag="RH058_001432"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV3137037.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31256..32548
                     /locus_tag="RH058_001433"
     CDS             31256..32548
                     /locus_tag="RH058_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3137038.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPRSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32673..33080
                     /gene="cueR"
                     /locus_tag="RH058_001434"
     CDS             32673..33080
                     /gene="cueR"
                     /locus_tag="RH058_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV3137039.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33081..33539)
                     /locus_tag="RH058_001435"
     CDS             complement(33081..33539)
                     /locus_tag="RH058_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV3137040.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33536..34453)
                     /locus_tag="RH058_001436"
     CDS             complement(33536..34453)
                     /locus_tag="RH058_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV3137041.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34599..35276
                     /gene="fetA"
                     /locus_tag="RH058_001437"
     CDS             34599..35276
                     /gene="fetA"
                     /locus_tag="RH058_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV3137042.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35263..36042
                     /gene="fetB"
                     /locus_tag="RH058_001438"
     CDS             35263..36042
                     /gene="fetB"
                     /locus_tag="RH058_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV3137043.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36105..36959)
                     /gene="cnoX"
                     /locus_tag="RH058_001439"
     CDS             complement(36105..36959)
                     /gene="cnoX"
                     /locus_tag="RH058_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV3137044.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37020..37829)
                     /gene="ybbO"
                     /locus_tag="RH058_001440"
     CDS             complement(37020..37829)
                     /gene="ybbO"
                     /locus_tag="RH058_001440"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV3137045.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(37819..38442)
                     /gene="tesA"
                     /locus_tag="RH058_001441"
     CDS             complement(37819..38442)
                     /gene="tesA"
                     /locus_tag="RH058_001441"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV3137046.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38413..39099
                     /locus_tag="RH058_001442"
     CDS             38413..39099
                     /locus_tag="RH058_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3137047.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39096..41510
                     /locus_tag="RH058_001443"
     CDS             39096..41510
                     /locus_tag="RH058_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3137048.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         9912 a        10946 c        11099 g         9649 t
ORIGIN      
        1 ttttttatga attttatccg tggttaatac tggttttcgt atgagatcct gagttggtgg
       61 ttcaattact ccttaatgtt cgtaggttat gcataaaaaa ggccgcttgc gcggccttag
      121 tgattacacg ttgtatcaat gatgatcgac agtatggctg tgctcgatat cttcattctt
      181 gcggctaaag cggcggcgaa ccacaacaaa gaataccgga acgaagaaga ttgccagtac
      241 cgttgcggtc accatcccgc ccattacacc ggtacctact gcgttctgcg cgccggaacc
      301 agcaccagta ctgataacca gcggcataac gccgaggata aacgccagcg aggtcatcag
      361 gatcggacgt aaacgcatcc gcactgcatc aagcgtcgct tcaatcagac ctttaccttc
      421 tttatccatc aagtctttgg cgaattcgac gataagtatc gcgttcttcg ccgacaaccc
      481 aatggttgtg agcaggccta cctggaagta aacgtcattg gtcaggccac ggaaggtggc
      541 agccagcaac gcaccgataa cccccagcgg aacgaccagc ataacggaga acggaatcga
      601 ccagctctcg tacagcgccg ccagacacag gaatacgaca atcaacgaaa tcgcgtacag
      661 tgaaggtgcc tggttgccgg agaggcgttc ctgataggac atccccgtcc agtcatagcc
      721 aacaccggta ggcagtttgc tcgccagttg ttccattagc tccattgctt caccggtact
      781 tttacccggt gccgcctggc ctaagatttc catggatggc aggccattgt aacgttccag
      841 acgcggcgaa ccgtactccc aacgagaaga ggagaatgcc gagaacggca ccatctgacc
      901 atcagcagca cgaacatacc agtcgccgat atcatccggc agcatacggt atttcgcttc
      961 tgacatgacg taaactttct tcacacgacc gcggtcgata aagtcgttca catagctacc
     1021 gccccatgca gcgcccagag tggtgttaat gtcgttgata gaaacaccca gcgcctgcgc
     1081 tttttcctgg tcgatatcaa tcttaaactg cggggtatct tccagaccgt ttggacgtac
     1141 gctggtcagc atatcagggt gcttcgctgc ttctgcaagc agctggttac gcgcctgagt
     1201 cagtttttcg tgaccaaggc cagcctggtc aatcagctca aagtcaaagc cggttgcagt
     1261 acccagttcc acgattgcgg gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga
     1321 gaaagcgcgt gttgcacgca tggtaatcgc ttcaactttg ttttcttcgc ccggacgatc
     1381 ggcccagtcc ttcaaggaaa cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc
     1441 gaagccgtta acggcgaaca ccgactcaac gttgttcttt tctttggtca gatagtaatg
     1501 cgttacctca ttgagcactt tctgcgtacg ttcctgcgtt gcacctgctg gcagctgaac
     1561 catggtcata aatacgccct ggtcctcatc tggcaagaag gagcttggca gacgcacgaa
     1621 cagataggcc atgccgacca cgatgatcag atacagcacc aggtaacgcc ccgtactgcg
     1681 cagaataccg cctacgctgt cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttaaa
     1741 ccagccgaag aagccttttt taccttcccc gtgatcacct ttggcaatcg gtttcagcat
     1801 ggtggcacaa agagccggag tcaggatcaa cgctaccagt accgacagcg ccattgctga
     1861 aacaatggta atagagaact gacgatagat agcaccagta gaaccgccaa agaaggccat
     1921 cggtacgaat accgccgaca gtaccatcgc gataccgacc agagcgccct gaatctgccc
     1981 catcgactta cgggtagctt cttttggcgg caaaccttct tccgccataa cacgctccac
     2041 gttttctacc acaacgatag cgtcatccac caacaagccg atagcgagca ccatcccgaa
     2101 cattgttagc gtgtttatcg agaagccaaa ggcggcaagg acggcaaagg tcccgagcaa
     2161 taccaccggt acggcaatgg tcggaatcaa cgtcgcgcgg aagttctgca ggaacagata
     2221 cataaccagg aacacgagga tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga
     2281 gattttcacg aacggcgtgg tgtcgtacgg gtaaacaatt ttcagacccg acgggaagaa
     2341 cggttccatc ttcgccagtt cagcacggat tgccgcagcg gtatccagcg cgtttgcacc
     2401 ggtcgccagc ttgatcccca gaccggaagc cggttggccg ttaaactctg cgatgatgtc
     2461 gtagttctca ccacccagct caatcttcgc cacatcacgc agcagtacgc gggaaccatc
     2521 ttgattcact ttcagcagga ttttgccgaa ctcttcagta gaggtcagac gcgtctgagc
     2581 aataatagag gcgttaagct gttggccttt caccggcggc gtaccaccga gctgacccgc
     2641 cgcaacctgg gcgttctgcg ctttgatggc ggtaatgaca tcaaccggcg ttagctggaa
     2701 tttgttcagc tcattcgggt tcatccagat acgcatcgcg tactgtgaac cgaacaactg
     2761 aacatcaccc acgcccgacg tacggctgat ggcatctttc atattcgccg ctacgtagtc
     2821 agagatatcc tcctgcgtca tggtgccatc ggtgttgata acgccgacaa ccatcaggaa
     2881 gctgctggat gatttctcaa cgctcacccc ttgctgctga acttcttgcg gcagcaacgg
     2941 catcgccagc tgcagtttgt tctgcacctg aacctgcgcg atatccgcat cagtaccaga
     3001 ctcaaaggtc agggtgatct gcacggtacc cgtggagtca ctgttagagg acatgtacat
     3061 caggttatcg ataccgttca tattctgttc gataacctgt gtcaccgtgt cctgcactgt
     3121 tttcgcatca gcgccggggt aggaggcgga gatcgttact gccggcggtg caatcgtagg
     3181 atattgcgcc accggcagtt tgaggatcgc cagcccccct gccaacatga tgataatggc
     3241 gatcacccac gcaaaaatcg ggcgatcgat aaagaaatta ggcatgtctt aacggctcct
     3301 gtttaagtta agacttggac tgttcaggct gagcaccgct tgcggcttgc tggttattat
     3361 cagcggtaac ttcttgtgct tttacctgga caccaggacg cactttctgc agcccactta
     3421 ttactacgcg atcgcctgct ttcagacctt ctgtcaccag ccacttatcg ccaatagcct
     3481 ggcttgcaac gatcggacgg gtttccactt tgtcatccgc gccaactacc agtacggtgg
     3541 catcaccacg cggcgtacgg gttacgccct gttgcgggac taaaatagcg tttggattaa
     3601 gcccttcttc cagacgtgcg cgcacgaaca tacccggcag cagagtgtga tccgggttcg
     3661 ggaagatagc gcgtagggtg atagacccag tggtctgatc aacggtaacg tcagagaatt
     3721 ccagcgtacc gtcctgcggg aacttaatgc cgtcactggt gatcagcgac actttggctt
     3781 tgccgttctc ttgtttcagc gtgccattcg ccagttcctg tttcaggcgc aggaagtcgt
     3841 tgctggactg ggtcacatca acgtagatcg gatcaagttg ctgcacggtt gccagcgcag
     3901 tcgcctgacc gttctgtacc aatgcgcctt ccgtcacgtt cgacttacca atgcgaccgc
     3961 taatcggaga ggtgactttg gtgtaagcca gattgatccg cgcagtttca acggcagctt
     4021 tcgccgcagt taccgcagca ttcgcctgtt gcgcatcagc cagagcctga tcgtactctt
     4081 gcttactgat gtactgagta ccgagcagtt tctgataacg attcaccgtc aattgcgcga
     4141 tattggctgc agcctgggct ttcgccagat cacctttcgc actgtcgtat gtcgcctgat
     4201 aggtcgcagg atcaatctga tagagagaga cacctgcttc gatgtcgcta ccttctttga
     4261 aattacgctt caggataatc ccgctaactt gaggacgaac ttctgcgatc cggtaggcac
     4321 tggtgcgacc cggaagctcg gttgtgatct gcagaggttc agttttgact gttactacgc
     4381 caacggcggg catctgctgg ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg
     4441 ctaagctgcc tgagagcatc agaacgaccg ccagaggcgt aaaccctctg tttttgttca
     4501 tatgtaaacc tcgagtgtcc gatttcaaat tggtcaatgg tcaaaagtta ataaacccat
     4561 tgctgcgttt atattatcgt cgtgctatgg tacatacatt cacaaatgta tgtaaatcta
     4621 acgcctgtaa attcacgaac atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg
     4681 ccaacacatc ctcgatgtgg ctctacgtct tttctcacag cagggggtat catccacctc
     4741 gctgggcgag attgcaaaag cagctggcgt tacgcgcggt gcaatctact ggcattttaa
     4801 agacaagtcg gatttgttca gtgagatctg ggaactgtca gaatccaata ttggtgaact
     4861 agagcttgag tatcaggcaa aattccctgg cgatccactc tcagtattaa gagagatatt
     4921 aattcatgtt cttgaatcca cggtgacaga agaacgacgt cgattattga tggagattat
     4981 attccacaaa tgcgaatttg tcggagaaat ggctgttgtg caacaggcac aacgtaatct
     5041 ctgtctggaa agttatgacc gtatagaaca aacgttaaaa cattgtattg aagcgaaaat
     5101 gttgcctgcg gatttaatga cgcgtcgggc agcaattatt atgcgcggct atatttccgg
     5161 cctgatggaa aactggctct ttgccccgca atcttttgat cttaaaaaag aagcccgcga
     5221 ttacgtcgcc atcttactgg agatgtatct cctgtgcccc acgcttcgta atcctgccac
     5281 taacgaataa ccctgaatct gactccagga tttttcctgg acattttcgt cgttgctatt
     5341 ctggttcact gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt cttcaggttc
     5401 agaaaccttc attcatcatg actatgttcc agtattacaa acgatcacgg cattttgttt
     5461 tttcagcatt tattgctttt gtttttgtct tgttatgcca gaacacggcg tttgcgcggg
     5521 cgtcatcgaa tggtgatctg ccgacaaaag cggacctgca ggcgcaactt gactcactaa
     5581 ataaacaaaa agatctttct gctcaggaca aactggtgca gcaggatctg acagatacat
     5641 tagccaccct cgataaaatc gatcgcataa aagaagagac agttcagcta cggcaaaaag
     5701 tcgctgaagc gccggaaaaa atgcgccagg cgaccgcggc gttaacagca cttagcgatg
     5761 tcgataacga cgaagaaacg cgcaaaattc tgagcacgct ttcgttgcgc cagctggaaa
     5821 ctcgcgttgc ccaggcgctg gacgatttgc aaaacgcaca aaacgatctg gcgtcttata
     5881 acagccagct ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg atgtataacg
     5941 cttcgcagca gctgcaacaa attcgcagtc gtctggatgg gactgatgtc ggcgagacag
     6001 ccttacgtcc cagccagaaa gtgttaatgc aggcccagca ggcgttgctg aatgcggaga
     6061 ttgaccagca gcgtaaaagc ctggaaggga acaccgtctt gcaggatacc ttgcaaaagc
     6121 aacgtgatta cgtgacggcg aacagcgctc gtctggagca ccagttacaa ctgttgcaag
     6181 aagcggtaaa cagcaagcgc ctgactttaa ccgaaaaaac ggcgcaggaa gccgtctccc
     6241 cggatgaagc cgcgcgtatt caggctaatc cgctggtgaa gcaggaactg gaaattaacc
     6301 agcagttaag tcagcgtctg attaccgcga ctgaaaacgg taatcagttg atgcagcaaa
     6361 acattaaagt caaaaactgg ctggagcggg cgctgcaatc ggaacgcaat attaaagagc
     6421 agattgccgt cctgaagggt agcctgctgt tgtctcgtat cctttaccag caacaacaaa
     6481 cgctgccctc ggcggatgaa ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg
     6541 aacagtttga agttaaccag cagcgtgatg cactcttcca gagcgatgcg ttcgtcaaca
     6601 aactggaaga aggtcacacc aacgaagtca acagcgaagt tcacgatgcg ttattgcaag
     6661 tggttgatat gcgtcgcgaa ttgctggatc aactcaacaa acagttgggt aaccagctga
     6721 tgatggccat taacctgcaa atcaaccagc agcagttaat gagcgtatcg aaaaacctga
     6781 aatccatcct gactcagcaa atcttttggg tgaacagtaa ccgtccaatg gactgggact
     6841 ggatcaaagc gttcccgcaa agcctgaaag atgaatttaa gtcgatgaaa atcacggtga
     6901 actgggaaaa agcctggcct gccgttttta tcgctttcct cgccggtttg ccgctgctgt
     6961 tgattgccgg gctgatccac tggcgtctgg gctggctgaa agcgtatcaa cagaaactgg
     7021 cttccgctgt gggttccctg cgtaacgata gccagctcaa cacaccaaaa gcgatcctta
     7081 tcgacctgat ccgcgcgctg ccggtgtgcc tgattattct cgcggttggc ctgattctgt
     7141 tgaccatgca gctcaacatc agcgaactgc tatggtcgtt cagtaaaaaa ctggcgattt
     7201 tctggctggt gtttggcctg tgctggaagg tactggagaa aaatggcgtt gccgtgcgtc
     7261 acttcggcat gccggagcag cagaccagcc actggcgtcg gcaaattgtc cgcatcagtc
     7321 tcgcgttgct gcctatccat ttctggtctg tggtggcaga actttccccg ctgcatctga
     7381 tggatgatgt gctggggcag gcgatgattt tcttcaacct gctgctgatt gccttcctgg
     7441 tatggccaat gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg cgactggtca
     7501 ccattaccgt gctgtcgatt atcccgattg cattgatggt gctgactgcc acgggttatt
     7561 tctacaccac gctgcgcctc tccgggcgtt ggattgaaac cgtttatctg gtgatcatct
     7621 ggaacttgct ctaccagacg gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct
     7681 ggcgtcgtgc gctggcgcgt cggcagaatc tggtgaaaga gggcgcagaa ggtgccgaac
     7741 cgccggaaga acccaccatt gcactggaac aggttaacca gcagacgctg cgtattacca
     7801 tgttgctgat gtttgcgctg ttcggtgtca tgttctgggc aatttggtcc gatttgatca
     7861 ccgtgttcag ctatctcgac agcatcacgc tctggcatta caacggcact gaagctggcg
     7921 ctgcggtggt gaaaaacgtc accatgggca gtctgttgtt tgcgattatc gcctcaatgg
     7981 tggcctgggc gttgattcgc aacctgcctg gtttactgga agtgctggtg ctctcgcgac
     8041 tgaatatgcg tcagggcgcg tcgtatgcca ttactaccat ccttaactac atcattattg
     8101 ccgttggtgc gatgacggtg ttcggatcgc tgggcgtctc ttgggataaa ctccagtggc
     8161 tggccgcagc attatccgta ggtcttggtt ttggtttaca agaaattttc ggtaacttcg
     8221 tctccggttt gatcattcta ttcgaacgtc cggtgcgtat tggcgatacg gtaaccattg
     8281 gtagcttctc ggggacggta agtaagatcc gtattcgtgc gacaacgatt accgatttcg
     8341 atcgcaaaga agtgatcatc ccgaacaaag cgtttgttac cgagcgtctg atcaactggt
     8401 cgttgactga cactactacg cgtctggtga tccgtctcgg cgtggcctat ggctccgatc
     8461 tggaaaaagt gcgtaaagtg ttactgaagg cggcgactga gcacccaagg gtgatgcacg
     8521 aaccaatgcc ggaagtcttc tttacggcat ttggtgccag cacgttggat catgagctgc
     8581 gtctgtatgt gcgtgaactg cgtgaccgta gtcgtactgt cgatgagctg aaccgtacta
     8641 tcgatcagct gtgccgtgaa aacgacatca acattgcctt taaccagctt gaagtgcatc
     8701 tgcacaacga gaagggcgat gaggtgacgg aagtaaaacg cgactacaaa ggcgatgacc
     8761 cgacgccagc ggtagggtaa aaacgaaagg gggcaacatt tagttgcccc gagattgcta
     8821 acaaagtgcg cgttgttcat gccggatgcg gcgtgaccgc cttatccggc ctacgaaacc
     8881 gcaagaattc aatatattgc aggagcggtg taggcctgat aagcgtagcg catcaggcag
     8941 ttttgcgttt gcccgcaacc ttaggggaca tttagcgacc ccatttattt ctcacttttc
     9001 cgcctcatca tcgcgcgtta atttcttttc ataatcacgc tttacaatat ccagcgcgcg
     9061 cagcacggta ctggcaggga tctgattttc ctccagcagc acaatcaaat cgacagccag
     9121 tttgacatcg tcaggggcat tttccagtga catattctct ccattgctag cgggttaaac
     9181 gcgctaacct gttttcgatt ttttccagcg catggcggca gcgtgccagg cgcgcttcat
     9241 aggcttccac ttcacgatgc agcgtttgct gttccacgag atcggtcacc cgcgccagac
     9301 gggctctgcg ttcggcaacc atctcacgta gccgccgctc aaaatcctga tgctgaatac
     9361 gtttacgctg ccagcgggca attttcggtg gtgcactgtc ccactcgcgc aatgaccagg
     9421 cggaggcttc acgcgcgatg gcttccagtt gtgccgccag atgttccgcc agccaggcca
     9481 cttgcggcag ctgttgctgc tcaactgcat gacgaagcgc agccagatta tcgcccgcct
     9541 catcgagaca agcctgcagt gttgtcgcac gggtttgaaa aaggtgtctg tcaaaacggg
     9601 cacttagcgt ggcaaattgc gctaccgggg cacaacgctg acgcagcgta gcgagctgtc
     9661 cttccagttt ttccagcagc agggcggttt tcacgataag acctcaatga aaatgataat
     9721 tgttatgcta aagtagccac tctgtaagct gacaacactc aatatgcaac gaatcatttt
     9781 aatcatcatt ggctggctgg cggtagtgct gggtacgctg ggcgtggtat taccggtatt
     9841 accgacgacg ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt
     9901 tcacgcctgg ttgctgtacc gctcatggtt tggcagctat ctacgtttct ggcagaaaca
     9961 tcatgcgatg ccgcgcggcg tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc
    10021 catttctctg tggttcgtcc agatgccacg ggtgcgcatc atgttgctgg taattctcgc
    10081 ctgtttgctt ttctatatgt ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg
    10141 aagcacaaca atcgcagttg caattattgc gtacagccag tacattctgg cgttttcgag
    10201 cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc
    10261 gtgagtacca ccgtaacaag caggcataca cttatgaccg cgactgcaca gcagcttgag
    10321 tatctcaaaa atagcatcaa aagcattcag gactacccaa aacccggcat tcttttccgc
    10381 gatgtcacca gcttactgga agacccgaaa gcttacgctc tcagcatcga cttgctggtt
    10441 gagcgttaca aaaatgcggg cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg
    10501 tttggcgctc cggtagctct gggtctgggc gttggctttg taccggtccg taaaccgggc
    10561 aaactgccgc gtgaaaccat cagtgaaact tacgacctgg aatacggcac cgatcagctg
    10621 gagatccacg ttgatgccat caaaccgggc gacaaagttc tggtggtgga cgatctgctg
    10681 gcaaccggcg gcactatcga agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg
    10741 gctgacgctg cgttcattat caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa
    10801 cagggcatta ccagctacag ccttgtcccg ttcccgggcc attaattatc gccagtcttg
    10861 tgctgcccac gctacggaca gcacaagatg tgcattcagc ctcgccgttc tgacggggct
    10921 gtgttagcat taccccttcg tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt
    10981 atcaggtctt agcccgaaaa tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac
    11041 atgtgctgac cgcactggcg aacggcttgt cgttagggcg tattcatcat gcttatcttt
    11101 tttccggtac ccgtggcgtc ggaaaaacct ctatcgcccg actgctggcg aaggggctaa
    11161 actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg
    11221 agcaggggcg ctttgtcgat ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag
    11281 atacccgcga cctgctggat aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt
    11341 atctgatcga cgaagtgcat atgctgtcgc gccacagctt taacgcactg ttaaaaaccc
    11401 ttgaagagcc gccggagcac gttaagtttc tgctggcgac gaccgatcca cagaaattgc
    11461 cggtgacgat tttgtcgcgc tgtttgcaat ttcatctcaa ggcgctggat gtcgagcaaa
    11521 ttcgccatca gcttgagcac atcctcaacg aagaacatat cgcccacgag ccgcgggcgc
    11581 tgcaattgct ggcgcgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc
    11641 aggcgattgc cagcggtgac ggccaggttt caacccaggc ggtcagtgcg atgctgggta
    11701 cgcttgacga cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc
    11761 gtgtaatggc gctaattaat gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg
    11821 tggaaatgct tggcctgttg catcgtattg cgatggtaca actttcgcct gctgcacttg
    11881 gcaacgatat ggccgccatc gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga
    11941 cggatatcca gctttactat cagacgttgt tgattggtcg caaagaatta ccgtatgcgc
    12001 cggaccgccg catgggcgtt gagatgacgc tgctgcgcgc gctggcgttc catccgcgta
    12061 tgccgctgcc tgagcctgaa gtgccacgcc agtcctttgc acccgtcgcg ccaacggcag
    12121 taatgacgcc aacccaggtg ccgccgcaac cgcaatcagc gccgcagcag gctccgactg
    12181 taccgctccc ggaaaccacc agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc
    12241 agggagcaac caaagcaaaa aagagtgaac cggcagccgc tacccgcgcg cggccggtga
    12301 ataacgctgc gctggaaaga ctggcttcgg tcacagatcg cgttcaggcg cgtccggtgc
    12361 catcggcgct ggaaaaagcg ccagctaaaa aagaagcgta tcgctggaag gcgaccactc
    12421 cagtgatgca gcaaaaagaa gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac
    12481 atgaaaaaac gccggaactg gcggcgaagc tggcggcaga agccattgag cgcgacccgt
    12541 gggcggctca ggtgagtcaa ctttcgctac caaaactggt cgaacaggtg gccttaaatg
    12601 cctggaaaga ggagagtgat aacgcagtat gtctgcattt gcgctcctct cagcggcatt
    12661 tgaacaaccg cggtgcacag caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa
    12721 cggttgaact gactatcgtt gaagatgata atcccgcggt gcgtacgccg ctggagtggc
    12781 gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga gtccattatt gcggataata
    12841 atattcagac cctgcgtcga ttcttcgatg cggagctgga tgaagaaagt atccgcccca
    12901 tttgatcgta agcacagctt acgttcgtca tccttaacgt gattgagaga gaaacctatg
    12961 tttggtaaag gcggtctggg taacctgatg aagcaagccc agcagatgca agaaaaaatg
    13021 cagaaaatgc aggaagagat cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg
    13081 gtaaaagtga ccatcaacgg tgcacataac tgccgtcgcg tagagatcga cccgagcctg
    13141 ctggaagacg acaaagagat gctggaagac ctggtggctg cagcattcaa cgacgcagca
    13201 cgtcgtatcg aagaaacgca gaaagaaaaa atggcttctg tatcctccgg aatgcagctg
    13261 ccgcctggct ttaagatgcc gttctgatgc aaaccagccc gctgttaaca cagcttatgg
    13321 aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc
    13381 tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt
    13441 cggaaatcgg ccactgcgcc gattgccgca ctttcaccga acaggaagtc tgtaacatct
    13501 gttcgaatcc gcgtcgtcag gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca
    13561 tctacgccat tgagcagacg gggcagtttt ctggtcgtta ttttgtgttg atggggcatc
    13621 tgtcaccgct ggacggcatc ggtccggatg atatcgggct tgatcgtctg gaacagcgtc
    13681 tggcagagga aaaaatcact gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag
    13741 ctaccgctaa ctacattgcc gagctttgcg cgcaatatga cgtggaagcc agccgaatcg
    13801 ctcatggcgt accggttggc ggcgaactgg aaatggtcga tggcaccact ctgtcacact
    13861 cccttgccgg gcgtcataag attcgttttt aagcaaacga gagcaggatc acctgctctc
    13921 gcttgaaatt attctccctt gtccccatct ctcccacatc ctgtttttaa ccttaaaatg
    13981 gcattattga ggtagaccta catgaaagga caagaaactc gtggttttca gtcagaagtg
    14041 aaacagcttc tgcacctgat gatccattct ctctattcca ataaagaaat cttcctgcgt
    14101 gagcttatct ctaacgcctc cgatgcggcg gacaagctgc gtttccgcgc gctctctaac
    14161 ccggacctgt acgaaggtga tggcgaactg cgcgttcgtg tctctttcga taaagacaag
    14221 cgcacgctga ccatctcaga taacggcgtg gggatgaccc gcgatgaagt gatcgaccat
    14281 ctggggacta tcgctaaatc cggcaccaaa tcattcctcg aatccctggg ttctgaccag
    14341 gcgaaagaca gccagctgat cggtcagttc ggtgttggtt tctactctgc gtttatcgtg
    14401 gccgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa aaccagaaaa tggcgtgttc
    14461 tgggaatcgg ctggtgaagg tgaatacacc gttgccgaca tcaccaaaga agatcgtggt
    14521 actgaaatca ccctgcatct gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg
    14581 cgttccatca tcagcaaata ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc
    14641 gaagagaaag acggcgaaac cgttatctcc tgggagaaaa tcaacaaagc gcaggcgctg
    14701 tggactcgta acaagtcgga aatcaccgat gaagagtaca aagagttcta caaacacatc
    14761 gcccacgact ttaacgatcc gctgacctgg agccacaacc gcgttgaagg taagcaggag
    14821 tacaccagcc tgctgtacat tccgtcccag gctccgtggg atatgtggaa ccgcgatcat
    14881 aaacacggcc tgaaactgta cgtccagcga gtgttcatca tggacgacgc agaacagttc
    14941 atgccgaact atctgcgctt cgtgcgtggt ctgattgact ccagcgatct gccgctgaac
    15001 gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc
    15061 aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc
    15121 ttctggcaac agtttggcct ggtactgaaa gaaggtccgg cggaagattt cgctaaccag
    15181 gaagcgatcg ccaaactgtt gcgttttgct tctacccata ccgattcttc tgcgcagacc
    15241 gtgtctctgg aagactacgt ttcccgcatg aaagaagggc aggagaaaat ctactacatc
    15301 actgcagaca gctatgcggc agcgaagagc agtccgcacc tggaactgct gcgtaagaaa
    15361 ggcatcgaag ttctgctgct ttccgaccgc attgatgagt ggatgatgaa ctatctgact
    15421 gagtttgacg gtaaaccgtt ccagtctgtg tctaaagttg acgagtcgct ggaaaaactg
    15481 gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag cactgactcc gttcatcgac
    15541 cgtgtgaaag ccctgctcgg cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc
    15601 gatacgccag cgatcgtttc gaccgacgcg gatgaaatga gcacccagat ggcgaaactg
    15661 ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca tcttcgaact gaacccggat
    15721 cacgtactgg tgaaacgtgc ggcagatact gaagatgaag cgaagttcag cgagtgggta
    15781 gaactgctgc tggatcaggc gctgctggca gaacgcggca cgctggaaga tccgaatctg
    15841 tttattcgtc gtatgaacca gctgctggtt tcctgatgtc atgccggatg accttcgtgt
    15901 catccggcat ttttcttttc atcatctgca ctttccgcaa attatctcgc cattaaccgt
    15961 ttcagcctca ggtgcctttc ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt
    16021 tcaaaaaatt cgacacattt taaggggatt ttcgcaatgc gtatcattct gcttggcgct
    16081 ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg agaaatatgg tattccgcaa
    16141 atctccactg gcgatatgct gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa
    16201 gcaaaagaca ttatggatgt tggcaaactg gtcaccgacg aactggtgat cgcgctggtt
    16261 aaagagcgca ttgctcagga agactgccgt aatggtttcc tgttggacgg cttcccgcgt
    16321 accattccgc aggcagacgc gatgaaagaa gcgggcatca atgttgatta cgttctggaa
    16381 ttcgacgtac cggacgaact gatcgttgac cgtatcgtag gccgccgcgt tcatgcgccg
    16441 tctggtcgtg tttatcacgt taaattcaat ccgccgaaag tagaaggcaa agacgacgtt
    16501 accggtgaag aactgactac ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg
    16561 gttgaatacc atcagatgac tgcaccgctg atcggctact actccaaaga agcggaagcg
    16621 ggtaacacca aatacgcgaa agttgacggc accaagccgg ttgctgaagt tcgcgctgat
    16681 ctggaaaaaa tcctcggcta attcgaaagc gcgcacggac agtcccctcg ccccctcggg
    16741 gagagggtta gggtgagggg aacaggcccg cacaagcaaa cttatcagca atctcaggcc
    16801 ggatattcat tcggcctttt acaaaaacct caatctgcgc tattcctttt tctgatttga
    16861 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
    16921 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
    16981 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
    17041 acgcgtggtt gatacctcac ggttgttatg gtggccgttg ctgcgcggcg tgattttgcc
    17101 gctgcgctcg ccgcgtgtgg cgaagctgta cgcctcggtc tggatggaag acggctcgcc
    17161 gctgatggtt tacagccgcc agcaacagca ggcgttggca caacgtttac cggatacgcc
    17221 agtggcgctg ggcatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct
    17281 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
    17341 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
    17401 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
    17461 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
    17521 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
    17581 tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc
    17641 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
    17701 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
    17761 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa
    17821 atatgaatat attccagcgc ttaatgccac gccggaacat atcgaaatga tggctaatct
    17881 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
    17941 gtcggcggtt ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
    18001 tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc
    18061 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
    18121 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
    18181 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
    18241 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc
    18301 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
    18361 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
    18421 gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga
    18481 aagggggtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
    18541 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
    18601 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
    18661 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
    18721 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccga
    18781 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
    18841 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
    18901 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    18961 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    19021 taaacgtaaa tccaaacatt acttccccgt aaatgcacgc gatccgctgc ttcagcaatt
    19081 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    19141 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    19201 actggtgatt gaggatgacg tagccgaagc gctttatcag gagctaaaac agaaaaacct
    19261 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct
    19321 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagtta
    19381 tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc tacaaggcgt
    19441 ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    19501 tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag atgtgattgc
    19561 cggagcctcg gcactggttc tcacctcata tctggtgcgt tgcaagccgg gtgaacccat
    19621 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    19681 gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    19741 agatcacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    19801 cgatccgttg ttggcatctg acaaggcgct ggactgggta gatctggtgc tgtgcaccgc
    19861 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    19921 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    19981 gcgccacaag gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg
    20041 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    20101 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    20161 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    20221 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    20281 cagcctggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg ccagataagg
    20341 cgttcacgcc gcatctggca tttggctctc gatgcccgat gcgacgctgg cgcgtcttat
    20401 catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc gtcaccactt
    20461 cacccgccgg tggcgtttcc agcagttcca gcatagtacg ggcaatttca cgttcaccca
    20521 tcactacctg attcgcgcca cgttcggtga tatacgtcac ttcatcgtca taatgggcgc
    20581 gggcgataat ctcaatatcc gggtttttcg cgcgggcaga tgccacaatc tcgcctgctt
    20641 cataaccgtt gggaatcgtc aggatcagcc attttgcaca ttccagatgc gccagttgca
    20701 taatttcttc gttcgccgca ttgcccaata ctgcgcggac cccgcgctct cgcagctcat
    20761 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    20821 cccccagcag gctgcctaca cgaccgtaac ccaccagaag cgcatggttg caaatatcca
    20881 ctgggatctg cttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    20941 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    21001 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    21061 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    21121 ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac gacttaccaa
    21181 acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    21241 caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcagcg
    21301 tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg gcaaagaacg
    21361 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    21421 gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    21481 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcactttgc
    21541 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    21601 ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
    21661 accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct tcaagtgcgc
    21721 gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata ccggtcatta
    21781 acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg gctatctggg
    21841 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaaatgca
    21901 aaccgacgcc gaacatcagc aaaataacgc ccagttcagc cagttccggc gcaagcttgg
    21961 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    22021 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    22081 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    22141 gttattgtcc atttttggcc ggggaaacca aaattacagg taatagttta tgacaatttc
    22201 attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta gctgcaaaaa
    22261 aagcacggat cggaaaacag aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
    22321 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
    22381 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
    22441 cgatactggt gtgatcggcg ataagcccca gaactgccgc tcccagacct cccataccaa
    22501 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagc agctcctgag
    22561 cgtaaaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
    22621 cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
    22681 tagagcccca aatcacatat ttccgcccaa ttttatcccc tacaggcccg ccgatcaccg
    22741 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgagcattc tggatagata
    22801 atccgaattt ttgcatcaga taaaaggtgt aatagctgct gatgctcgcc atatagaaat
    22861 atttcgagaa aatgaggatt aacagaatgc tgaccgccag taccactttg ttgcgcggca
    22921 gtggattgat aatcgtcgct ttgggttttc ctttattcat tcggtgctgt gccgagtacc
    22981 aacggctgat ttgcgccaac accacgatcg ccagcagtgc cgcaagcaca aaccaggcaa
    23041 cgttgccttt gccataaggc gcgataatca ccgccgccag caagggtccc agggaactgc
    23101 caaagttgcc gccgacctga aagatagatt gcgccaggcc atgccgcccg ccggaagcca
    23161 tacgggctac gcgagaagat tccggatgaa agaccgatga accggtaccg accagcgccg
    23221 ccgccagcag aactgcgcca aaactgcccg ccagcgcaag cagcaccaga ccacttaagg
    23281 taaagcacat gccaattggc aacgaccacg gcatcggata tttatcggtc cagtagccga
    23341 ccactggttg cagtagcgaa gaggcgagct ggaaggtgag ggttatcatg ccaatctgca
    23401 taaatgtcag agaaaattct gactgaagca gcggataaat cgccagaatc agcgattgga
    23461 tcatgtcgtt cagcagatgt gagaggctga tagcacctaa aataccaaac gatgttcggg
    23521 ccttggtcgt tgacgcagcc gcgcccgcca caggctgggg ttgttcactc attgccatag
    23581 gaaagtcact ttttcagggt tgcgatgtaa agaatgatct tatttgtgat tattacccga
    23641 ctaacatacc tgtatgcgtc gcctgaagga agtctcaacg ccgaatacag aatttctaat
    23701 ctggatgcag atttatcttc accggacgca gacttgtcta tgatgtcgcg tcatactatt
    23761 tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa attattgcag cggggcgtgg
    23821 cgttagcgct gttaaccaca tttacactgg cgagtgaaac tgctctggcg tatgagcagg
    23881 ataaaaccta caaaattaca gttctgcata ccaatgatca tcatgggcat ttttggcgca
    23941 atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct ggtggatggt atccgcaaag
    24001 aggttgcggc tgaaggcggt agcgtgctgc tactttccgg tggcgacatt aacactggcg
    24061 tgcccgagtc tgacttacag gatgccgaac ctgattttcg cggtatgaat ctggtgggct
    24121 atgacgcgat ggcgatcggt aatcatgaat ttgataatcc gctcaccgta ttacgccagc
    24181 aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat ctaccagaaa agtactggcg
    24241 agcgcctgtt taaaccgtgg gcgctgttta agcgtcagga tctgaaaatt gccgttattg
    24301 ggctgacaac cgatgacaca gcaaaaattg gtaacccgga atacttcact gatatcgaat
    24361 ttcgtaagcc cgccgatgaa gcgaagctgg tgattcagga gctgcaacag acagaaaagc
    24421 cagacattat tatcgcggcg acccatatgg ggcattacga taatggtgag cacggctcta
    24481 acgcaccggg cgatgtggag atggcacgcg cgctgcctgc cggatcgctg gcgatgatcg
    24541 tcggtggtca ctcgcaagat ccggtctgca tggcggcaga aaacaaaaaa caggtcgatt
    24601 acgtgccggg tacgccatgc aaaccagatc aacaaaacgg catctggatt gtgcaggcgc
    24661 atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc gaaatgaaaa
    24721 tggttaacta ccatctgatt ccggtgaacc tgaagaagaa agtgacctgg gaagacggga
    24781 aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa ccagcaaatg atctcgctgt
    24841 tatcaccgtt ccagaacaaa ggcaaagcgc agctggaagt gaaaataggc gaaaccaatg
    24901 gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gaccaatatg gggcggttga
    24961 ttctggcagc ccaaatggat cgcactggtg ccgactttgc ggtgatgagc ggaggcggaa
    25021 ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtgctgaaa gtgcagccat
    25081 tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga ggtgattgat tacctgaccg
    25141 ccgtcgcgca gatgaagcca gattcaggtg cctacccgca atttgccaac gttagctttg
    25201 tggcgaaaga cggcaaactg aacgacctta aaatcaaagg cgaaccggtc gatccggcga
    25261 aaacttaccg tatggcgaca ttaaacttca atgccaccgg cggtgatgga tatccgcgcc
    25321 ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg ctgaaagcgt
    25381 atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt gaggtgagct
    25441 ggcagtaatc cgaaagtgcc ggatgtttgc atccggcaca atgcttaatc gcggcgggcg
    25501 atatcagcaa atttggcatc gaggatcttt gccagatcgc ctgccgccag ttcgatatcc
    25561 agtccgcgct tgccgccgga aacataaata gtggcaaatt cttgtgcggg ggcgtcgata
    25621 atcgttggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag gtatcccgtc
    25681 gaacgctgcg cggccatcgg atcggccatc tcaactttct tggcacccag cgcttttgct
    25741 acttttttaa gatccagttg acctgcgacc ggcgtaacgg ccacggcaag gtgtttcata
    25801 tcaccgttca ctgccaccag cagcgttttg tagacctgat ccggattcaa acctaatttt
    25861 ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt atggatttga
    25921 aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat tttcctgcct
    25981 taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag agtcatcttg
    26041 cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc gtcgcttaaa
    26101 gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag cgatattggc
    26161 cattttttta gcgcaacatt tgcggcaaat tcccttctcc atacaggtgt agtgcaccga
    26221 ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag gcgagatttc
    26281 gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc gttatatcat
    26341 tattttgcgg aggtgcattc agccaccagc tcatcatttg ttgcagcaac cgtgcgttgg
    26401 tatgccagtg ggtcagcgta tcgtccagca gcgccagtcc tttgtcaggg agctggagca
    26461 acatggcaat ctggttttca gccccttcca gttcaatcac gggtttatgt tgttgcttcg
    26521 ccgcctgcaa tagctggtaa tcgataccgt attctgcccg cagccccagt ttttgtgcct
    26581 gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg ggtagaaaag agtgagggag
    26641 aaatgcccat ctcctggcta atgtgctgca ggttttggag ttgctcctcg ctaatgcgct
    26701 cttccagcgc ctcgcaggca ggcaaattag caaaaggcgt atcgctggtg gaaacatccg
    26761 cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg ggcaggggag
    26821 ccatatcgtg gctacccata tgaatactgc caatcagatg aaaatggcga ttgccgggga
    26881 gggtgatatc gatggcaggc caggagtaat gattaccgcg cagcgcggcc caaagcgttt
    26941 ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct agcgcgcggt
    27001 gagggatggc gcaaatggag ttggggactt gtttaatgtg tttgtatgat tcagtatgtt
    27061 cttgcatcgc tattcacagg gaagcaacag ttaaaaacca tgaaacaggc aacaagaaaa
    27121 ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga tttgaaaatc
    27181 aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtgcact gatcaataac
    27241 aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga taccacagtc
    27301 gattttaggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa caacatgcgc
    27361 acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg ccgtgaagag
    27421 cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat ctcgtaggcc ggataaggcg
    27481 tttacgccgc atccggcagt catgcgtcga tgccaaatgc gccgccctaa agcagcgcat
    27541 ccgcaatgac gtacttattc cttcggttta aaccgcagca accggttggc gttactcact
    27601 acggtaatcg acgagagcgc cattgccgct ccggcaacta ccgggttaag cagtgttcca
    27661 gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tgctgttgta gataaaggca
    27721 ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca
    27781 acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc aacatcactg
    27841 ccgccaccca tcgcaatgcc gacatccgcc tgggccagcg ctggcgcatc gttaatgccg
    27901 tcgcccacca ttgccacctg acgtccttca ctttgcaggc gtttaatcgc ttcggcttta
    27961 ccgtccggca gcaccccggc gatcacttca tcaatcccgg cttctttggc gatcgcattg
    28021 gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt atgcaggcgt
    28081 tgcagcgccg ccacgctatc actacgcaat ggatcgcgta ctgccagcag ggctactgct
    28141 ttcccgtcaa tcgccagcag cacaggcgtt gctccttgcg atgcctgagc agtaatctcc
    28201 gcttcgatag ctttggtacc aacctgttgc tcatttaaca gcgcctgatt gcccagcaat
    28261 aacgcatgac cttcagcttc accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg
    28321 acctgcggca gctgcatatc acctgcttta tcgaggatcg cccgtgccag cggatggctg
    28381 gaaccttgct ccagtgccgc cgccagacgc aatgcctgcg cttcatcaac atcagcaaac
    28441 gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt atcgaacacg
    28501 acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac
    28561 tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagccc cagcgcacac
    28621 ggacaggcga taatcagcac cgtggtggct atcaccaggg tatagacaat ctgcggtgcc
    28681 ggaccaaaga aataccagat tgctgcactg atcagcgcaa tcaccaccac cacaggtaca
    28741 aatacggctg agattttatc cgccagctga ccgatttctg gcttgctgct ctgggcctgg
    28801 cgcaccatgc gaatgatccg tgacagcgtg gtatggctgc caaccgcact ggcgcgaaat
    28861 agcacgctac cgtcctgtac cactgtcccg gcatggacgc ttttgccttc gcctttttgc
    28921 tgcgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc
    28981 tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gcaacatacc tggctgcact
    29041 tccgccagag gcacgctttt ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt
    29101 aaatcgagta atttctccag cgccttagaa gaacgctgac gtgcgcgtgc ttccagcata
    29161 tggccgagat tgatcagacc gataatcatc gcgctggctt cgtaataaag atgtcgcgct
    29221 tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca cgccacgcca
    29281 gtacccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct tttccatgca
    29341 ctgcggtaaa aatggccgcc ggcgaaaacc atcaccgcca gggttatcag gccgataacc
    29401 aaccacaggc tgcggttgtc agcggtgacc atcatgttat cgccaatcat cccccagacc
    29461 atcaccggga tacccaccgc cagtgcgaca attgcctgcc agcggaagcg cttcatcgta
    29521 gcaacggcgg tttcctgctg gcgctcacgg cgtttcgcgt catcttcaat cgcttccgcg
    29581 ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact gcccatcacc
    29641 agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac gctttgcagc
    29701 gcattttgta cgcgggtgac acagctggcg cagctcatac cgctcagcag caactgctgg
    29761 ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc
    29821 gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga tgcgtcataa
    29881 cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt aacgtgcgct
    29941 tcagtgatag acacatccgc ttgttccaca tccgggcgct gttcgagact ttctttcacg
    30001 cgtttaacgc agtgaccgca ggacaggccg tccagggtca ggtcgatagt ttgtgacata
    30061 aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt ccagcaaggg
    30121 gaaggtcaag aaattaataa accaggcgag taaaagtccg taaagattaa aaaattggct
    30181 caatttgcat caggattaga catttatctc tttgttttcc tgtagttaag ttgcgggtgc
    30241 taagttaaat caggatgcct gaaaatcggc accggggtga ggaattacct cccgcatcta
    30301 taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca gtggatcagg
    30361 cttacaccca atttcactcg cttaacggcg gtcaaaatgc cgactacatt ccctttctgg
    30421 cgaatgtgcc tggtcaactg gcggcagtgg ctatcgtgac ctgcgatggc aaggtctata
    30481 gtgcgggtga cagtgattat cgctttgcgc tggaatccat ctcgaaagtc tgtacgttag
    30541 cccttgcgtt agaggatgtc ggcccgcagg cggtacagga caaaattggc gctgacccga
    30601 ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa ccgctttcgc
    30661 cactggtaaa tgctggcgcc attgccacca ccagcctgat taacgctgaa aatgttgaac
    30721 aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag gtagcgctct
    30781 ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc attgcctggc
    30841 tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac gtgtacaccc
    30901 gtcagtgctc cacgctcctc aatactgttg aactggcaac acttggcgcg acgctggcgg
    30961 cggggggagt gaatccgctg acgcataaac gcgttcttca ggccgataac gtgccgtaca
    31021 ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg gcatatcgcg
    31081 ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc cctggtgtga
    31141 tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt cgcggtcaaa
    31201 aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc tgatcatgat
    31261 gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg
    31321 cattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg cgttgctgat
    31381 ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt tttccggtta
    31441 tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggcattatcg acttctttcg
    31501 tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc tgttgacgct
    31561 ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc agtttttgca
    31621 tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca ttgcggtgat
    31681 gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg
    31741 cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc tgcaaccggc
    31801 gcatatttcc gtctctgcgc cccccagatc cggtgcgttc ttctcctgta tcgggataac
    31861 cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc
    31921 gcaggtcatt atgccgcggg cgtttctggt ggcgattggc gttaccacgt tgctttatat
    31981 ctcgctggca ctggttttgc ttagcgatgt atcggcatta gagttagaaa aatatgccga
    32041 taccgccgta gcgcaggctg cttctccgct gctcggacat gtgggttatg tgatcgtcgt
    32101 catcggcgct ttactggcga cggcttcagc cattaacgcg aacctgttcg ccgtgtttaa
    32161 catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata aatccctgtg
    32221 gcggcagagt acctggggca acatcattgt cgtggtgttg attatgctga tgacggcggc
    32281 gctaaattta ggctcactcg ccagtgttgc cagcgccacc tttttgattt gctacctggc
    32341 ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc cgattcttat
    32401 cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagtc tgtggtccca
    32461 gggtagccgt gcgttgatat ggattattgg ctcactctta ctcagcctta ttgtggcaat
    32521 ggtcatgaag cgcaataaaa ccgtataaca tctctctgtg cgcagtactt cctgtattat
    32581 tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc
    32641 tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta
    32701 ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg acgccgccga
    32761 tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa ctgaccttac
    32821 tgcgtcaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg gtgaatctgt
    32881 ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag aaggtggcgg
    32941 agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg gcactggcga
    33001 atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc tccggctgct
    33061 gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt tatcccttct
    33121 atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct gacaggccat
    33181 gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc cagcacaaaa
    33241 cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg
    33301 cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt caggatggca
    33361 aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag gccggtaatc
    33421 accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc ggctgccagc
    33481 agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa ctccatcatg
    33541 gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca atcgacccca
    33601 tcaggctgct ggcctctaat ggcatcatca ctactttgct gttactggag gaaccgatct
    33661 gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga atatcaccgg
    33721 aggcgatggc ttcagacacc attttggtgg cgcgggcttc tgcttcagcg gaacgttcac
    33781 gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg atttgcgact
    33841 gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct tcaagaatgt
    33901 aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata agctctgccg
    33961 gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg ttggtggctt
    34021 catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa
    34081 gttccattga acccaacacg gtacggatgt tagtcatggt caggttgatg atcgccagct
    34141 ccagattgct gacttcataa gccgcgcgcg gcgcgtcaat cacctgaata aaacagactg
    34201 cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg atatcgagca
    34261 cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc accagactga
    34321 gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat tgatagccct
    34381 gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga
    34441 taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa aattgctgct
    34501 tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc tgcgaaggat
    34561 aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc
    34621 ttcagctaca aaacgtagga tatctggcgg gtgatacgaa gattcttaat aacatcaatt
    34681 tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta
    34741 cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta ctgtttgaag
    34801 gggaggatgt cagcacacta aagccagaaa tctaccgcca gcaagtctct tactgcgccc
    34861 agacaccgac gctgtttggc gatacggtat acgataatct gatctttccc tggcagatcc
    34921 gtaaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc gctttgccgg
    34981 acagcatttt gacgaaggat atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat
    35041 tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata accagtgcgc
    35101 tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa
    35161 atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg gataaagtga
    35221 ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc gcataatatt
    35281 actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt aattagccat
    35341 aaagaaaaac tggcgctgga gaaagatatt ctctggagcg tcgggcgagc gataattcag
    35401 ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc cagcctgaca
    35461 ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa
    35521 tatattgcta aagcttttat ctcatcgttt gttgccatta cggtcggggc gggaattacc
    35581 ctggcggtgc tgattctctc agggtcgatt gaatttatcc cgatgcaggt gatccctatc
    35641 gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa caatttaggg
    35701 caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg tgcgacgccg
    35761 aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat tccgacggtc
    35821 gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg gctgatattt
    35881 gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt tatgctgctc
    35941 tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa gttttataat
    36001 tcgcgccacc agttggtggt gacgcaattg aagaagaaat aatacgatgc cggatgcgca
    36061 gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata caacaatgca
    36121 tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagtgc agcgaggatc
    36181 tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg cagatgcccg
    36241 aacagcaact ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag agccagttgc
    36301 gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg ttgaatttcc
    36361 ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg gtagcgggtg
    36421 tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga acggttcagc
    36481 gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga caactgccag
    36541 gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc
    36601 tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc gcggatcgcc
    36661 tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa cagatacacg
    36721 gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc gcagtccagc
    36781 ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat cggggttaac
    36841 tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac
    36901 tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc tacggacatg
    36961 gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg cgcttcaact
    37021 cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttaag caccattact
    37081 gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat aaaagcatgg
    37141 cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat gccgggattt
    37201 tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt acgaatggga
    37261 ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg cagcgcgtct
    37321 gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg cgtggagatt
    37381 aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa catcgcgggt
    37441 aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc
    37501 tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt gttaaagatc
    37561 ccatacagac aattatcggt cagggcgatc acttcgtcgg ctgcgcgatc aacactttct
    37621 ggcgaatcca gatcgatcaa cacgccggta aatcccatgc tgttcatacg ctcaacatca
    37681 tccggtttcc ggcaacctgc cagcacatga aaaccctggc gttttaattc gagcgcgctt
    37741 tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat aactttacct
    37801 gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact gcttcgccat
    37861 ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat cctgcatcca
    37921 ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat caaactcttt
    37981 ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat agtttgcagg
    38041 cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct gcaaaatctg
    38101 gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat tgccgcccag
    38161 ttcaaccagc acccaacgcg gctgatgctg tttcagcaga gccggaaggc gcgccagtcc
    38221 ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac tctgccactt
    38281 atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg cgctcaggct
    38341 atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa
    38401 caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc
    38461 ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt caaacgtggc
    38521 gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct ggcgatcctc
    38581 gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc gctacataat
    38641 atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca
    38701 tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc tctgctgcgc
    38761 ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca gttagggctg
    38821 ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca acgagtggcg
    38881 ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc caccggcaac
    38941 cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa ccgtgaacat
    39001 ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg cgaccgctgc
    39061 ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg
    39121 aatggcgttc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg gtggcctgcg
    39181 tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag caaagccgtg
    39241 agtttatggc gggcgatcgg gcgttgcgca gttcacgcga agtgccgcaa gcgtggctgg
    39301 aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc acaatgacct
    39361 ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt
    39421 atggagatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg
    39481 ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac gtgggcgatg
    39541 ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt aaccccttcc
    39601 agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga gccgtgcagc
    39661 cggggagtcg ggtcacctgg cgttataaat tcggcggcaa cgagaaccag ctcgacggct
    39721 atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt ctggaacagg
    39781 acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc
    39841 ttctgacctt gctgctggca gtggcagcgg tggcggtagc gatgaatcat tactgtcgca
    39901 gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg caactgcgta
    39961 agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc ggtggggcca
    40021 taggtctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac
    40081 taccgccagc cagcctctgg ccgtggctgt gggcgcttgg caccatgacg gtcatctcgc
    40141 tgctggtggg gctacgacca taccgcttgt tgctggcaac gcagccttta cgcgtattac
    40201 gtaatgatgt ggtagcgaac gtctggccgc tgaagtttta tctgccgatt gtcagtgtgg
    40261 tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg gcggtgctgg
    40321 cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg ctgaatgtac
    40381 ttcgccgcat gacgctgaaa tcgctgcctc tgcgcctggc ggttagccgc ctgttacgtc
    40441 agccgtggtc aacgttaagt cagctttcgg cattttcgct ctcctttatg ctgctggcgc
    40501 tgttgctggt attgcgtggc gatctgctcg accgctggca gcagcagcta ccgccagaaa
    40561 gcccgaacta ctttttaatt aacatcgcca cagaacaggt agcaccgcta aaagcgttcc
    40621 tcgcggaaca tcatatagtc ccggaatcat tttatccggt agtgcgggcg cggctgacgg
    40681 cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaatcgc gaactcaatc
    40741 ttacctggca aaatacgcgg cccgatcata atccgattgt cgccggtaac tggccgccaa
    40801 aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg
    40861 ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca
    40921 aagtggactg ggaaagtctg cggcctaatt tctattttat tttcccggaa ggggcattag
    40981 acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga
    41041 cgcaactcaa ccgccagttc ccgaccatta gcctgttaga tattggcgcg attttaaaac
    41101 aggtcggtca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg gtgctggtca
    41161 ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggaatgcgc cagcgtcatc
    41221 aggagctggt ggtatggcgc acactcggtg cggggaaaaa actgctgcgc acgacattgt
    41281 ggtgtgagtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa
    41341 cggcactggc agtgttgcag gcgaaagtgt ttgattttcc gtgggagcca gactggcgat
    41401 tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg
    41461 gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga tgagagtaaa
    41521 taaggggcgt tctggttgaa tggaacgcct tgtttaaatt aattctacaa tatgtttcat
    41581 tgatgatgtt attgaattgg tgattt
//
LOCUS       DAPHDX010000028        40745 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPHDX010000028 DAPHDX010000000
VERSION     DAPHDX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.028"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(82..981)
                     /gene="lysO"
                     /locus_tag="RH058_001444"
     CDS             complement(82..981)
                     /gene="lysO"
                     /locus_tag="RH058_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV3137049.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(1438..2133)
                     /gene="aqpZ"
                     /locus_tag="RH058_001445"
     CDS             complement(1438..2133)
                     /gene="aqpZ"
                     /locus_tag="RH058_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV3137050.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            2559..4217
                     /locus_tag="RH058_001446"
     CDS             2559..4217
                     /locus_tag="RH058_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV3137051.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(4214..5170)
                     /locus_tag="RH058_001447"
     CDS             complement(4214..5170)
                     /locus_tag="RH058_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDV3137052.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            5321..6436
                     /gene="macA"
                     /locus_tag="RH058_001448"
     CDS             5321..6436
                     /gene="macA"
                     /locus_tag="RH058_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV3137053.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            6433..8379
                     /gene="macB"
                     /locus_tag="RH058_001449"
     CDS             6433..8379
                     /gene="macB"
                     /locus_tag="RH058_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV3137054.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(8452..8676)
                     /gene="cspD"
                     /locus_tag="RH058_001450"
     CDS             complement(8452..8676)
                     /gene="cspD"
                     /locus_tag="RH058_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV3137055.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            8999..9319
                     /gene="clpS"
                     /locus_tag="RH058_001451"
     CDS             8999..9319
                     /gene="clpS"
                     /locus_tag="RH058_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV3137056.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            9350..11626
                     /gene="clpA"
                     /locus_tag="RH058_001452"
     CDS             9350..11626
                     /gene="clpA"
                     /locus_tag="RH058_001452"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV3137057.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(11970..12057)
                     /locus_tag="RH058_001453"
     tRNA            complement(11970..12057)
                     /locus_tag="RH058_001453"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12021..12023),aa:Ser,seq:gga)
     gene            complement(12311..12529)
                     /gene="infA"
                     /locus_tag="RH058_001454"
     CDS             complement(12311..12529)
                     /gene="infA"
                     /locus_tag="RH058_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV3137058.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(12814..13518)
                     /gene="aat"
                     /locus_tag="RH058_001455"
     CDS             complement(12814..13518)
                     /gene="aat"
                     /locus_tag="RH058_001455"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV3137059.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(13560..15281)
                     /gene="cydC"
                     /locus_tag="RH058_001456"
     CDS             complement(13560..15281)
                     /gene="cydC"
                     /locus_tag="RH058_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV3137060.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTHVA
                     NRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDETLAEIL
                     RRVGLEKLLEDTGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(15282..17048)
                     /gene="cydD"
                     /locus_tag="RH058_001457"
     CDS             complement(15282..17048)
                     /gene="cydD"
                     /locus_tag="RH058_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV3137061.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETEIASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(17171..18136)
                     /gene="trxB"
                     /locus_tag="RH058_001458"
     CDS             complement(17171..18136)
                     /gene="trxB"
                     /locus_tag="RH058_001458"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV3137062.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            18681..19175
                     /gene="lrp"
                     /locus_tag="RH058_001459"
     CDS             18681..19175
                     /gene="lrp"
                     /locus_tag="RH058_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV3137063.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            19310..23377
                     /gene="ftsK"
                     /locus_tag="RH058_001460"
     CDS             19310..23377
                     /gene="ftsK"
                     /locus_tag="RH058_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV3137064.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            23532..24143
                     /gene="lolA"
                     /locus_tag="RH058_001461"
     CDS             23532..24143
                     /gene="lolA"
                     /locus_tag="RH058_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV3137065.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            24154..25497
                     /gene="rarA"
                     /locus_tag="RH058_001462"
                     /gene_synonym="mgsA"
     CDS             24154..25497
                     /gene="rarA"
                     /locus_tag="RH058_001462"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV3137066.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            25588..26880
                     /gene="serS"
                     /locus_tag="RH058_001463"
     CDS             25588..26880
                     /gene="serS"
                     /locus_tag="RH058_001463"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV3137067.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            27119..29563
                     /gene="dmsA"
                     /locus_tag="RH058_001464"
     CDS             27119..29563
                     /gene="dmsA"
                     /locus_tag="RH058_001464"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV3137068.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            29574..30191
                     /gene="dmsB"
                     /locus_tag="RH058_001465"
     CDS             29574..30191
                     /gene="dmsB"
                     /locus_tag="RH058_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV3137069.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            30193..31056
                     /gene="dmsC"
                     /locus_tag="RH058_001466"
     CDS             30193..31056
                     /gene="dmsC"
                     /locus_tag="RH058_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV3137070.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(31092..31718)
                     /locus_tag="RH058_001467"
     CDS             complement(31092..31718)
                     /locus_tag="RH058_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3137071.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            32033..33181
                     /locus_tag="RH058_001468"
     CDS             32033..33181
                     /locus_tag="RH058_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137072.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            33391..34821
                     /locus_tag="RH058_001469"
     CDS             33391..34821
                     /locus_tag="RH058_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3137073.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(34822..35730)
                     /locus_tag="RH058_001470"
     CDS             complement(34822..35730)
                     /locus_tag="RH058_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3137074.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            35830..36420
                     /locus_tag="RH058_001471"
     CDS             35830..36420
                     /locus_tag="RH058_001471"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDV3137075.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(36502..37242)
                     /gene="pflA"
                     /locus_tag="RH058_001472"
     CDS             complement(36502..37242)
                     /gene="pflA"
                     /locus_tag="RH058_001472"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV3137076.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(37434..39716)
                     /gene="pflB"
                     /locus_tag="RH058_001473"
     CDS             complement(37434..39716)
                     /gene="pflB"
                     /locus_tag="RH058_001473"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV3137077.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(39771..40628)
                     /gene="focA"
                     /locus_tag="RH058_001474"
     CDS             complement(39771..40628)
                     /gene="focA"
                     /locus_tag="RH058_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV3137078.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        10204 a         9937 c        10760 g         9844 t
ORIGIN      
        1 ataaaaggga tttttcatgc aactttaagg gagattgatt tagcgcaatt ttggcggcag
       61 ggatctaccg ccagagaggt attacgcaga gaaaaaggcg atgaggatcg gcactaacag
      121 gctaagaata aaaccgtgaa caattgccgc cgggaccata tccagcccgc cagtacgttg
      181 aagaacgggc agggtgaaat ccattgatgt ggcaccgcat aagcccagtg cagtagagcg
      241 gctgcggcga atcagcccag ggatcaacat aatagcaatc agttcacggg ccagatcatt
      301 aaaaaacgcc gcgctcccga ttaccggacc aaaagattcg gtcaataaaa taccggaaag
      361 agaataccag ccgaaaccgg aggccattgc cagcgcggta ttgatgggga gatcaagaat
      421 aaaggcgtta attaaaccac caattaatga actgacaacc accaccacgg cgacaatcat
      481 tccccggcga ttaaggacaa tctgctttaa ggtcatgcca ttattgcgca actgaatacc
      541 aacgaggaaa agtagcaaaa ttaacgtgta ttcactggct tcggtcgcgt gttgtaagaa
      601 agccagtcca ctcagaccaa tggcaaaacc aatcactact acgccgcaca gttttagcga
      661 ctccagcgcc atcgcaatac gcgacgggag tttttcttgc tgatggtggt tgcgccacgg
      721 caggcctcgc tccagccaca tcagggcggc aatattacac agtaaaataa cggtaatact
      781 gacggcagaa taatgcagaa tcgccaacag gttactggcg aggttatcga gaaacgccag
      841 actgataccc ataaaaaaga gaataaggta aaccatccag cttaatagct gattaataac
      901 ttttaacgca gcttgttggc gaagcggaat gaggtaaccc acaatcaggg gaaccagaat
      961 gattaacagc ccagaaaaca tgaaaaccca gtccttgcaa agatgaagtc gaaatgcgcg
     1021 atgacacact actgaaagcg gaaggacgag taaagttgca attaaaagga aatgttatgc
     1081 atcagagggg gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg
     1141 caatgccata taaagccact ggcgcaggga gcgcgcaggg ggcggccagt cgccgccgcc
     1201 ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc
     1261 ccttcggcta ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat
     1321 ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccc
     1381 gtgcggtttg taacgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta
     1441 attacgtttt tccagcaggg tccggtaaat cagaccaccg ataatgccgc cgacaattgg
     1501 caccacccag aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc
     1561 ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga atactaatta agtgaatcag
     1621 ggttaaggcc agaccaatag cgatcggcgc aaaacctgcc ggcgcgaatt tgtcggttgc
     1681 gccgtggatc accaacagga aacctgcact caataccagt tcaactacca gcgcggaaag
     1741 catggaataa ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc
     1801 cgcgtcaaaa cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc
     1861 gccgacaacc tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc
     1921 ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcaaa
     1981 ggccatcgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac caatgcctaa
     2041 ttccgggaag cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt
     2101 accaaaacat tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg
     2161 tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg
     2221 gctattttaa aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt
     2281 agggcaacag cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa
     2341 aagatttaat cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt
     2401 tgtttttgca gacttatcag caagagggag tataacgcga ttattcgctc atttttcaga
     2461 catttgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg
     2521 ccagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa
     2581 ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga
     2641 ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc
     2701 cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca
     2761 acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac
     2821 atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc
     2881 accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc
     2941 gcagttttct cgataaagac ggacatccga ttgatgtcga ggatattaac gatcaggcac
     3001 gccatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta
     3061 ttcgtaacgg cacggtgcca aatgttccca atgtggaagt caccgcgcgc cagctcgatt
     3121 tcctcgcccg tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg
     3181 gactttccgc aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg
     3241 cgcgatatcg tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg
     3301 atatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg
     3361 gcctgtttgc aactttattg caggcaaaag gcacattgcg actggataaa gacgcccgtc
     3421 cattgttgct gattgaagat ccagaaaccc gtttacaccc cattatgctt tcagttgcct
     3481 ggcatctgtt gaatcttctg ccattgcagc gcattgccac caccaactcg ggtgagttgc
     3541 tttcgttaac tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg
     3601 cctggcgtct ggggccaagt ggcttgagca ccgaagacag ccgacgcata tcctttcaca
     3661 ttcgttttaa ccgtccgtca tcgctgtttg cacgctgctg gttgctggtg gaaggggaaa
     3721 cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag
     3781 ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc
     3841 gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg
     3901 ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt
     3961 taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc
     4021 gcgtggcgca aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga
     4081 tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg
     4141 gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc
     4201 gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca
     4261 tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc
     4321 gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgatt
     4381 cccagaaagc gttgtaatca gcgtggatct tgccttcttt atcgcgataa cgcaggctgc
     4441 ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa
     4501 acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg
     4561 cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca
     4621 tcgttctttt cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt
     4681 cgctgttgcg gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca
     4741 gctcaagcgt gaactgctcg ccgtttttgc cttccagctt cgccagttgc agcccggggg
     4801 tattcaaata taagctgaat tcttccgccg acatacatcc gcggagtaac gcataatggt
     4861 aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca
     4921 gacgcacagg cagtcgcggc tggcgcgtta acaatacatt gagattaggc cagtgggaaa
     4981 gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct
     5041 ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa
     5101 gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg
     5161 acatattcat gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc
     5221 aaacatcaat agtgtgatta ctaacgtaaa ttttaggggt ttgttgatat ttcgttgaag
     5281 ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt
     5341 gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag
     5401 aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca
     5461 gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca
     5521 ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca
     5581 gcttttaggg gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac
     5641 gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt
     5701 gacgtattcc cgtcagcaac gtctggcaca aacgcaggcg gtttcacagc aggatctcga
     5761 caccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat
     5821 caagcgcaat caggcttctc tcgatacggc taaaaccaat ctcgattaca ctcgcatcgt
     5881 tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc
     5941 cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc
     6001 gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt
     6061 acttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtactac cgacgccgga
     6121 aaaggttaac gacgctattt tctattacgc ccgttttgaa gtccccaacc ccaatggttt
     6181 gctgcggctg gatatgactg cgcaagtgca tattcagctc accgatgtga aaaatgtgct
     6241 gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt
     6301 gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga
     6361 tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc
     6421 aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc
     6481 ggtgatgagc aggttgaggt gctgaagggc atcaccctcg atatttatgc cggtgagatg
     6541 gtagcgattg ttggcgcttc aggttccggt aaatcgaccc tgatgaatat tctcggctgt
     6601 ctggataagg ccaccagcgg cacctatcgc gtcgccggtc aggatgttgc cacactggac
     6661 gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat
     6721 ttactttcgc atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt
     6781 gagcggaaac agcggctgct tcgcgcccag gggttactgc aacgactggg gctggaagac
     6841 cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg
     6901 cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccaaccgg cgcactggac
     6961 agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg
     7021 gtgattatcg tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt
     7081 cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg
     7141 acggagcctg ttgtcaacac ggtgtctggc tggcggcagt ttgtcagcgg ttttaacgag
     7201 gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg
     7261 ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc
     7321 aaacaaatgg tgctggcgga tattcgttct ataggtacga atactattga tgtctatccc
     7381 gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta
     7441 atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg
     7501 cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat
     7561 tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac
     7621 ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa
     7681 gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg
     7741 gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac
     7801 agcacgatgt ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg
     7861 aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc
     7921 cacggtaaga aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag
     7981 accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggt
     8041 ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc
     8101 attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc
     8161 gtactggttt gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc
     8221 accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc actgctgctg
     8281 gcgtttctct gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg
     8341 gcacgactgg atccagtaga tgctctggca cgagagtaat ttttgagata aaaatgccag
     8401 ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac
     8461 tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg
     8521 gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt
     8581 ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt
     8641 ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt
     8701 atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact
     8761 ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg ggacaggggg
     8821 caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg
     8881 ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa
     8941 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
     9001 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
     9061 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga
     9121 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
     9181 gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc
     9241 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
     9301 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
     9361 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     9421 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     9481 ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     9541 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt
     9601 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggtaac
     9661 cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt
     9721 gcgtaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa
     9781 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     9841 tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg
     9901 aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg
     9961 tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc
    10021 ggaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac
    10081 gatttactct ctcgatatcg gttctctgtt agcgggcact aaatatcgcg gcgactttga
    10141 aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat
    10201 tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggccagg tcgatgcggc
    10261 taacctgatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta
    10321 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
    10381 tgatattact gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
    10441 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcag cggtagagct
    10501 ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgacg ttatcgacga
    10561 agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
    10621 ggatattgag tccgtggtgg cccgtattgc gcgcattcca gagaagagtg tttctcagag
    10681 tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga
    10741 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
    10801 acataaaccg gttggttcgt tcctgtttgc cggccctacc ggggtcggga aaacagaggt
    10861 gacggtacag ctttcgaaag cgttgggcat tgagctgctg cgctttgata tgtccgagta
    10921 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
    10981 tcagggaggt ttgctgactg atgcggtcat caagcatcca catgcggtgc tgttgctgga
    11041 cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg
    11101 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac
    11161 caccaacgcc ggggtacgtg aaactgagcg taaatccatt ggtcttatcc accaggataa
    11221 cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct
    11281 cgacaacatt atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa
    11341 attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctcttg aagtgagcca
    11401 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat
    11461 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc
    11521 gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta
    11581 cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt
    11641 aggttggtga agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggagg
    11701 aggttcagac ccttttttta atgatgatgg taagttgttg ataattagtg ctgcgggaag
    11761 gtaaggataa aaaagggtgc tgcaggagaa tgggatggtt ttgctttatt aacaacgggc
    11821 taaacgtgta gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg
    11881 taaaaccttt gcgaacgcgt tcactgccgt acagacagat aaaatgcgaa aaaaaagctc
    11941 gcactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat
    12001 acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa
    12061 ttgttttgct accaaacctc atgggtggca acggggcgct actataggga gttggagtaa
    12121 aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc
    12181 agtatatcta catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca
    12241 caaaataaaa aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc
    12301 ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca
    12361 gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg
    12421 agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca
    12481 acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg
    12541 gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa
    12601 gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta
    12661 agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga
    12721 cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc gaccgcaatt
    12781 ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa acaagcatcg
    12841 tggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattaaggta
    12901 atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca
    12961 gtcgataagc ttaccgccat gaccgataaa ttcctcacag aataccagaa gcgccgtttt
    13021 agacgcattt tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac
    13081 gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt gggcatggcc
    13141 gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc
    13201 gcgatcgctg gcacagcctt caatgacctg accaaaagcg taattcatcg tgacacgata
    13261 gggcgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt ctggccatag
    13321 caccgcgcgg ggatcgggcg accaccagag gatggggtcg cctggagaaa accacggaaa
    13381 aataccacgc tggtaagcca ttaacaggcg cgcagggcta agatcgcccc caagtgccag
    13441 caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga
    13501 aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct
    13561 tacaaaccct gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta
    13621 ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg
    13681 cgaagtcgat gggtgaccat taacaccgtt ttctcatgca tcatttctgc aagcaattca
    13741 aggatctgac tttcggttgt ggcatctaag ccttcggttg gttcatccag caatactaac
    13801 ggcgcatcgt gcaatagcgc acgggcgata gccagacgac gcagttcgcc gccggaaagc
    13861 tgacgtccgc cttctcccaa ccaactgttg agccctgtgt cttcgagtag tttctccaga
    13921 ccgacgcgac ggagaatttc agcaagagtc tcatcactac tgccaggcga ggcgagtaac
    13981 agattatcac gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc
    14041 tgtcgtagag ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc
    14101 tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt
    14161 cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc
    14221 tgttgggatt gctccggata agtgaactga acatcccgta acgtcagcga aacgcgattg
    14281 gcaacatgag tttgggtatc agggaaggtg acttccggtt tttgatccgt taagtcagtg
    14341 atccgaacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc
    14401 agtgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga
    14461 gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca
    14521 atgagcagca ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc
    14581 agccattgaa tttctgtatt ctctaattgc gtgcgataac gatcgctggc accaaaaatg
    14641 gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga
    14701 tgagtcagat tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa
    14761 agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac
    14821 ccgattgtca ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg
    14881 taaagatgat cgagcgtatc aacatccgcc accacgcggt tgagcaattc gccctgacga
    14941 tagcgcgcca gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga
    15001 tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca
    15061 gtacgggtga ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac
    15121 agtccggcaa cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga
    15181 ccgatactgg cgagcagcgt gacaattgcc agcacgatac caagacttaa catccattta
    15241 tgacgtttat acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga
    15301 tgggccagta atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc
    15361 tcaataatcc ggccatcctg cataacccaa atgacatccc agtcagcaag atcttctaac
    15421 tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg
    15481 cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag
    15541 ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca
    15601 gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc
    15661 caggcgttat ccagcgctgc ttgtaattct tgttcgctgg catcaggtcg cgccagtagt
    15721 acgttatccc gcaatgttgc tgccggtaat tgtgggtttt gcccaaccca ggagagatgt
    15781 ttacgccatg attctggtga taaatcgcgt aattctatcc cgttgattcg taatgatccc
    15841 tgatatgaga gaaaaccaga aagcgcgttc agcagtgagc ttttacctga accgctgcga
    15901 ccaaccaaca ctgcacgttg acctgctggc aaagtaaagt tcagcggtcc ggccagcgtt
    15961 ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggttgat
    16021 gctatttccg tctcaccgcg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga
    16081 ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat
    16141 ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca
    16201 gtatcgtagt gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc
    16261 agagcaattg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa cgccagccgt
    16321 agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca
    16381 gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc
    16441 ccacttaagc gagcaagagc gagaaagtta cgccggttag catcggcagc ccccatccca
    16501 accagcgcca taaacaacgg aattaacggt gctgtgccca gcagaatgag cgccgcagcc
    16561 cagttagagg ggaagatcgc caccacaatc agcaacggca ccgacactgc cagcgccatt
    16621 tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccaacgtc
    16681 gcccagctcc ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg
    16741 agaacctgac ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg
    16801 cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc
    16861 agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac
    16921 caggcctggg caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc
    16981 caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgggat
    17041 ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac
    17101 ggggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc cttctttact tttgttactg
    17161 atttgtaaaa ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg
    17221 ccatgcagcc tgtaccggcc gaagtaatgg cctgacgata aatatgatcc atcacgtcac
    17281 ccgcggcaaa gacgccaggg atgctggtct gggtggcatt accatgaata cccgactgca
    17341 ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta ttcgggctgt
    17401 gaccgatagc aacaaacaga ccagcaacgt cgagtgactc gatgttatcg ctgttttgcg
    17461 tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac
    17521 ggttggtgtg cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga
    17581 ttttttccgc gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag
    17641 acagatacag cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt
    17701 tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt
    17761 cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc
    17821 aagtgtattc gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt
    17881 tgatatgatc aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca
    17941 ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact tccgtggtgg
    18001 tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg
    18061 ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt
    18121 gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg
    18181 aatttacaga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc
    18241 atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt
    18301 tataagccga ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact
    18361 aaaaatcgat gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa
    18421 aaaaaacagt attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta
    18481 gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc
    18541 aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct
    18601 atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg tcagacagga gtagggaagg
    18661 aatacagaga gacaataata atggtagata gcaagaagcg ccctggcaaa gatctcgacc
    18721 gtatcgatcg taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc
    18781 tttctaaacg tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa
    18841 gacaagggtt tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac
    18901 ttctggtatt cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca
    18961 ataccgctgt acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg
    19021 actacctgtt gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa
    19081 ccctgctgcg tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca
    19141 agcagagtaa tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt
    19201 attttgatta cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt
    19261 gtccgtttta gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga
    19321 atacactgaa gacaaagaag tcacattgac aaagttaagt agcggccgtc gccttctgga
    19381 agcgttgctg atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt
    19441 taacccttcg gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg
    19501 tgggatgccc ggtgcgtggc tggcagatac gctgttcttt atttttggcg tgatggctta
    19561 caccattccc gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagtga
    19621 cgaatacatt gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgcttatcct
    19681 tacctcctgt ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg
    19741 cgtcattggc agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac
    19801 tattgcgctg ctctgcgttt gggctgcggg cctgacgctg ttcaccggtt ggtcatgggt
    19861 gaccattgct gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg
    19921 tacccgtcgc gatgatacct gggtcgatga agatgaatat gaagatgacg aagagtatga
    19981 agatgaaaac cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct
    20041 agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc
    20101 tgcgttgttc tccggtaagc ggatggatga tgacgaagag attatctaca ctgcacgcgg
    20161 tgtggctgcc gacccggacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata
    20221 tgacgaatac gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc
    20281 tgctgctacc acggcgacac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc
    20341 gcctgttgcc tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc
    20401 gggtccacaa acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagtc
    20461 acaatatgcg cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccgca
    20521 gcagccgtat tatgcacctg cagctgaaca acctgcgcaa caaccttact acgctcctgc
    20581 ggcggaacaa cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg
    20641 taacgcctgg caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac
    20701 tgagcaaact tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaatccgt
    20761 tgaacagcag cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc
    20821 gccgctttac tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc
    20881 ggcctggtat caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgtt
    20941 gaaagcgcct tctgttgcag cagtacctcc agtagaagcc gctgccgctg tttctccgct
    21001 ggcatctggc gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc
    21061 agtcttcagt ctggcaaata gcggtggacc gcgtccgcaa gtcaaagagg ggattggccc
    21121 gcagttgcca cgaccgaaac gtatccgcgt gccaactcgt cgtgaactgg cgtcttacgg
    21181 tattaagctg ccctcacagc gtgcggcgga agagaaagcc cgtgaagccc agcgcaatca
    21241 gtatgattct ggcgatcagt ataacgatga tgaaatcgat gcgatgcagc aggatgaact
    21301 ggcacgtcag ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt
    21361 gcccgtaaac gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc
    21421 gcaaactcaa caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct
    21481 ggatgatttt gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt
    21541 gtttacgcca attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca
    21601 gtatcagcag ccgcaacaac cagttccgcc acagcagcaa tatcagcagc cgcaacagcc
    21661 ggttgcgccg cagccgcagt atcagcagcc acaacagcag gttgcgccgc agccacaata
    21721 tcagcagccg caacaaccgg ttgcgccaca gccgcaatat cagcagccgc aacaaccagt
    21781 tgcgccgcag ccgcagtatc aacagccgca acaaccggtt gcaccgcagc agcaggatac
    21841 cctgcttcat ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac
    21901 gccgctgcct tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac
    21961 ctttgcgctt gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa
    22021 agccgatgtc gtcaattact ctccggggcc ggttatcact cgctttgaac ttaacctggc
    22081 tcctggcgta aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc
    22141 gacggtggcg gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt
    22201 accaaataaa aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg
    22261 cgataatccg tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt
    22321 tgccgatctg gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc
    22381 tgtcggtgtg aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg
    22441 tttcatcatg atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct
    22501 gttaacggaa gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa
    22561 cgagatggag cgtcggtata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta
    22621 taacgaaaaa attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa
    22681 gccgggtgac agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt
    22741 gttggttgac gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat
    22801 agcacgtctg gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg
    22861 tccatcggtt gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt
    22921 taccgtatcc agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact
    22981 gctggggatg ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca
    23041 tggtgctttt gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg
    23101 tcgcccacag tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg
    23161 tttcgatggc gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga
    23221 aaaacgcaaa gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc
    23281 agcgcgtatt atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg
    23341 taatcgtgaa gtgctggccc caccgccgtt tgactaatta atgcattgcc ggataaggcg
    23401 cggtagcgtc gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa
    23461 gctgattatt agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt
    23521 gaggaataat gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa
    23581 gcagcgtttg ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct
    23641 tccacgccag cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc
    23701 agggcgatct gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg
    23761 aaagcattct ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag
    23821 ctacggcaac ctggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca
    23881 accagtccag cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga
    23941 cgccgaaagc cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca
    24001 caatccatca gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat
    24061 cccagcaaaa tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca
    24121 cggtagatga tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg
    24181 gataatactt ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc
    24241 ggccagcaac atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat
    24301 ttacattcta tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg
    24361 attgcccgct atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa
    24421 gaaattcgcg aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt
    24481 ctttttgttg acgaagttca ccgtttcaac aaaagccagc aggatgcatt tctgccacat
    24541 attgaagacg gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt
    24601 aattcggcac tgctttcccg tgcccgtgtc tatctgttga aatccctgag tacagaggat
    24661 attgagcaag tactaactca ggcgatggaa gacaaaacgc gtggctatgg tggtcaggat
    24721 attgttctgc cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc
    24781 cgggcgttaa atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag
    24841 cgggtcctga agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat
    24901 aacaaaggcg atcgctttta cgatctgatt tccgcactgc ataagtcggt acgtggtagc
    24961 gcacccgatg cggcgctgta ctggtatgcg cgaattatta ccgccggtgg cgatccgtta
    25021 tatgtcgcgc gtcgctgtct ggcgatagcg tctgaagatg tgggtaatgc tgatccacga
    25081 gcgatgcagg tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt
    25141 gaacgcgcca ttgctcaggc gattgtttac ctagcctgcg cgccaaaaag caacgctgtc
    25201 tacactgcgt ttaaagccgc gctggcggat gctcgcgaac gtccggatta tgacgtgccg
    25261 gttcatttgc gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat
    25321 cgttacgctc atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa
    25381 atagcacaaa cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa
    25441 aagctcgcct ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt
    25501 tatcgttgcg gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc
    25561 atttaattcg ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca
    25621 gacgcagtcg ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc
    25681 gctcttgaag agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt
    25741 aactcccgat cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta
    25801 cgtctggaag tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct
    25861 ttacaggctg aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg
    25921 ccggtaggta aagacgaaaa tgacaacgtc gaagtcagcc gctggggcac cccgcgtgag
    25981 tttgactttg aagttcgtga ccatgtgacg ctgggtgaaa tgcactctgg cctcgacttt
    26041 gcagccgcag ttaagctgac cggttcccgc tttgtggtaa tgaaagggca gattgctcgc
    26101 atgcaccgcg cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt
    26161 gagaactatg ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg
    26221 aaatttgctg gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac
    26281 tatgcgctga tcccaacggc agaagttccg ctgaccaacc tggtacgcgg tgaaatcatt
    26341 gatgaagatg atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc
    26401 ggttcatatg gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa
    26461 atggtgcaga tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat
    26521 gcagaaaaag tcctgcaact gctgggccta ccgtaccgta aaattatcct ttgtaccggc
    26581 gacatgggct ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac
    26641 acctaccgcg agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag
    26701 gcacgttgtc gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct
    26761 ggtctggctg ttggtcgtac gctggttgcg gttatggaaa actatcagca ggctgatggt
    26821 cgtattgaag taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa
    26881 tacccaattt ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata
    26941 ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat
    27001 ctctttgatt ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat
    27061 aattacacaa tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat
    27121 gaaaacgaaa atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa
    27181 aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg
    27241 gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag
    27301 cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga
    27361 aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt
    27421 tcgcgcctgt ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa
    27481 atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga
    27541 agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga
    27601 gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc
    27661 gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc ttaaccatta
    27721 cggtgactac tcttctgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc
    27781 agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt ttggtaataa
    27841 cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca
    27901 gaaatctaat gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg
    27961 cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta acggtctggc
    28021 gtatgtcatg atcaccgaaa acctggtgga tcaggcattc cttgataaat attgcgttgg
    28081 ctacgatgag aaaaccctgc ccgctagcgc gccgaaaaat ggccactata aagcttatat
    28141 tctgggtgaa gggccagatg gcgtggctaa aacaccgcaa tgggcctcgc aaatcaccgg
    28201 tatcccggca gagaaaatca ttcaactggc acgggaaatc ggcagtacca aaccggcgtt
    28261 tatcagccag ggatgggggc cacagcgcca cgccaacggc gaaatcgcga ctcgcgctat
    28321 ctcgatgctg gcgattctga ccggtaacgt cggtattaac ggaggcaaca gcggcgcgcg
    28381 tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac
    28441 cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacagcgct
    28501 gcgtgatggt gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta
    28561 tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg aaatccttca
    28621 ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa
    28681 atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcactgga
    28741 tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt
    28801 tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca
    28861 acagtttact gaaggccgta cacaggaaga gtggatgcgg catctgtatg cccagtcgcg
    28921 ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa
    28981 gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa
    29041 cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc
    29101 cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg
    29161 ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca
    29221 ctataaatct cgcgttcact caacttacgg caacgttgat gtgctgaaag cggcttgccg
    29281 tcaggaaatg tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa
    29341 agtcaggatc tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat
    29401 gatgccgggt gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt
    29461 cgataagggc ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg
    29521 gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa
    29581 cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg
    29641 cctgtaaaga ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg
    29701 ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc
    29761 tgtcgatttc atgtaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg
    29821 cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc attggctgcc
    29881 gctactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata
    29941 tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg
    30001 aatcttgccc gctgcgggcg ctggatttcg gtcctatcga cgagctgcgt aaaaaacatg
    30061 gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga
    30121 tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga
    30181 aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc
    30241 gggcaatgtg tagcaggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg
    30301 cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gtttatgggt gctgatgggc
    30361 attggcttta tcgcctctat gctccatctt ggttcaccaa tgcgcgcttt taactcgctc
    30421 aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc
    30481 gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg
    30541 ctgtggctga tagtgacgat ggttcttggc gtcatctttg tctggatgat ggtgcgtgtg
    30601 tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc
    30661 ctgacgatgt ttatgggcgg cccgttactg ggttacctgt tgttgagtct ggctggcgtc
    30721 gatggctggg cgatgcgtct gctgccagcc atttctgtac tggcactggt ggtgagtggc
    30781 gtgatgtcag tgatgcaggg cacagagctg gcaactattc atagttctgt gcagcaggcc
    30841 gcagcgctgg tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt
    30901 gccctgtgct tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta
    30961 tccgtctcat tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt
    31021 ttgcatatga ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc
    31081 ccggttctct tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa
    31141 tcaggaatat ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg
    31201 tgcagctcgc acgccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga
    31261 tcccatgccg aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaag
    31321 acatcaaacc cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc
    31381 acaccggcaa taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg
    31441 ttatcccagg cgttaatatt tcccgggcga gcaatgtaag gtgcatcggg gaattgtgct
    31501 ttcagttctg gaactaatgg accgttagga ccggtttcaa aactggtggt aagaatagta
    31561 ggtaagttga aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg
    31621 ggttcgatat cccgtacaag ggaaagtaaa ccagcctgat gatcaacaag caaaacggca
    31681 gcatcatttt tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca
    31741 gtggtacgtc tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata
    31801 tgagtttgct gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga
    31861 tcaataaaaa caatcagatg agattaatca tctcttcagc attagcgtta tgaatgagta
    31921 atataaatcg ataataccgc cgctgcgata tgcgcgtatt ttcacccatt gacaatgttt
    31981 ttggcggtgg catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac
    32041 gtatacccgg cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc
    32101 ggtgttaaat acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt
    32161 aggcgttgtc agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta
    32221 tgtcattaaa cgcattggct ttaaccgcag ctattatctg gcctccttca tttttgccgc
    32281 tggctgtgcc ggccttggcc tgatgattgg tttctggagc tggttggcgt ggcgttttgt
    32341 cgctggcgtc ggctgtgcca tgatttgggt ggttgttgag agcgcgctga tgtgcagtgg
    32401 gacgtcacgt aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac
    32461 gtttttaggc cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg
    32521 ggttacaggt ttgacattgg cagggatctt accgctgttg tttacgcgtg tgctgaatca
    32581 gcaggctgaa aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg
    32641 gcttggcgtg aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat
    32701 gccgttgtac ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt
    32761 actggtcagt gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg
    32821 tcgactgctg gtgttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta tcgcgatgct
    32881 tagccaggcg gcgatggccc ctgcgttatt catcctcggt gccgctggct ttacgctata
    32941 tccggtggcg atggcctggg cttgtgagaa agttgaacat catcaactgg tggcgatgaa
    33001 ccaggcctta ctgttgagtt atactgtggg aagtctgctt ggcccgtcat ttaccgctat
    33061 gctaatgcag aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat
    33121 ctatttgctg atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta
    33181 aatgaattca agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat
    33241 ctgacttgct ttattccaaa ttttattcgt ttaaaaataa aatgtgcagc aggttataat
    33301 tttgcatttc gcgatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat
    33361 gcccctttaa ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagttg
    33421 cgatggtaca acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt
    33481 aataactatg ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcactc
    33541 tatttcacac cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag
    33601 ggcggggtca gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg
    33661 tggacctact gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt
    33721 gcgctcggtt gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg
    33781 ttacaggggt taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg
    33841 aaatcgctga aaatcgtcgg ttctgtggca gggattgcga tgtttgttat gtcgctcctg
    33901 tatgtggcga tggcggtaac cgcgcctgcg attactgaag tgcatattgc gaccacaaac
    33961 attacctggg aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg
    34021 ctggttttcg cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac
    34081 ccaggaaaag aatttccaaa agggatgcta tgcctggcgg tgatggttgc ggtttgtgcc
    34141 attctgggct cactggcgat ggggatgatg tttgactcgc gtaatatccc ggatgactta
    34201 atgacgaacg gtcaatatta cgcctttcag aagctgggcg agtattacaa catgggtaat
    34261 actttaatgg tgatttacgc cattgcgaat accctgggac aagtagctgc gctggtattc
    34321 tcgattgatg ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc
    34381 agtttatgtc gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggtg
    34441 ctggtggcaa ttctgattat gctgccgact ctcggcatcg gtgatatgaa caatctctat
    34501 aaatggctgt tgaatcttaa ctcggtagtg atgccgctgc gttatttatg ggtatttgtt
    34561 gcatttattg cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt
    34621 aataagcctc tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt
    34681 ttgacgggga tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg
    34741 ctgaatgttg caacgccgtt tgtgctggta ggattaggac tgattttccc gctgctggcg
    34801 cgtaaagcga atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc
    34861 cgtttttaca taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttggata
    34921 atagatctgg aagcctggac gctctgtgct ccagtcttcc agtacacatt ctaaacgacc
    34981 agtatcaagg tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa
    35041 aacggcctcc agttcagcat caacatcatc cagaatgata tttcctgcga cagcaatttc
    35101 cagctccttt gcaaattgcc agtggaaagg tttgccgctg ggataacgaa aaacaacaca
    35161 ttgatgatta agcagatcat gaggatggcg gggtttgcca tatcttgcaa agtactctgg
    35221 cgttgctgct acacataatt tcactggcgg gccgatcgcc acgctgatca tatctttttc
    35281 gacgatgcca ctaagtcgaa cgccagcgtc aaagccctgt tggacgatat cgacaagaga
    35341 gtcgtcagtt gtgagctcga ctttaatatc aggatattca cgtgtgaaac caaccagtaa
    35401 aggcattaaa aatatacgtg cagctacccg ggcggcattg attttgagcg ttcctgtcgg
    35461 tgtcagacga aaatcattca tctcatctaa catgatctga atttcgtcga aagctgggcg
    35521 caatcgttca tagagattag aacccgcttc cgttaacgat acgcttcggg ttgtccgatt
    35581 gaataagcga attttaagac gttgttccag tgttttaata ctatggctaa tggcggacga
    35641 ggataagcct aattcatcgc ccgctgcacg aaagctttga ttacgggcca cggcaaagaa
    35701 agtggcaaag tcagacatat tcatccgcat tagtgaaaat ccttcattaa ctcatcctga
    35761 atcatcacgt ttacaggatt atgccgcctg cgtaaagtgc gctccagaac ttaacgtgga
    35821 ggtaaaatta tgcagtctga acgtatttat ttggtatggg cccatcctcg tcatgattca
    35881 ttgaccgcac atattgctga tgcgatccat cagcgggcaa tggagcggaa aatacaggtg
    35941 acggaactcg atttatatcg gcgtaatttc aacccggtga tgacgtcgga agatgaacca
    36001 gactggaaga atatggataa acgttattct ccagaggttc atcagcttta ttcagagctg
    36061 cttgagcatg acacgttagt ggtggttttt cctctctggt ggtacagctt cccggcaatg
    36121 ctaaaaggat atattgacag agtatggaat aatgggctgg cttatggaga tgggcacaaa
    36181 ttaccattca ataaagttcg ttgggtggcg ctggttggag gagacaaaga atcatttgtc
    36241 cagatgggct gggaaaaaaa tataagcgat tatttaaaaa atatgtgcag ttatcttggt
    36301 attgaagatg ccgatgtcac tttcttgtgt aatacagtgg tattcgatgg ggaagaactt
    36361 cacgcgagct attatcagtc gttattatct caggtacggg atatggtaga tgcactataa
    36421 gatgtgttaa aaacgctgta gcagaatgaa gcgcggaata aaaaagcggc aactcaataa
    36481 agttgccgct ttacggggaa attagaacat taccttatga ccgtactgct caagaatgcc
    36541 tttcacgcgt tccatggtct ctttcttcgg tggtttaaca ccgtcgagtt tgtactcttc
    36601 acccattgcc acccatttgt gtttgcccag ctcgtggtag gggagaagct cgattttctc
    36661 aacgttgccc atatcacggg taaattcacc gaggcgatgc gctgaatcgt catcgtcaga
    36721 ccagcctggg acaacaacgt agcggatcca caccttcaca tttttgttcg ccagatattt
    36781 ggcgaactcc agcgtgcggt gattggaaac tccaaccaga ttttggtgga tctcgtcgtt
    36841 catctgtttg agatcgagca ttaccaggtc ggttacttcc agcagttcat caatcaccgg
    36901 atcgtaacga cgaacaaaac cgttggtgtc cagacaggta tgaatgcctt cttttttgca
    36961 ggcgcggaac cagtcacgaa caaactcagc ttgtaggatt gcctcaccgc cggatgcggt
    37021 aacgccgccg ccggaagcgt tcataaagtg gcgataggtc accacctctt tcattaaatc
    37081 ttcaacggta acttctttac cgccatgcgt atcccaggtg tcgcggttat gacaatacag
    37141 gcagcgcatc aggcagccct ggaaaaaggt gataaagcga atacctgggc cgtctacggt
    37201 tccacaggat tcaaaggagt gaatgcgacc aataactgac attgcggtgt ttctccagat
    37261 gtggcccatc tgaggccgtg ttggtgcgca gctcgaaggc tacgtcgagt ctgttttggc
    37321 agtcacctta gagtatagat agctgacaaa aaaggctctc gtgctaaaaa aggccccact
    37381 ttcgtggagc ctttattgta cgctttttac tgtacgattt cagtcaaatc taattacata
    37441 gattgagtga aggtacgagt aataacgtcc tgctgctgtt ctttagtcag cgagttgaaa
    37501 cgtactgcgt agccagatac acggatggtc agctgcggat atttttccgg gttttccatc
    37561 gcgtcgagca gcatttcacg gttcatcacg ttaacgttca ggtgctgacc accttcgatg
    37621 gatgcttcgt ggtggaagta accatccatc agaccagcca ggttggtctt acgaacttcg
    37681 tcgtctttac ccagtgcgtt cggaacgata gagaaggtgt aggagatacc atctttagcg
    37741 taagcaaacg gcagtttagc aacggaagtc agagacgcta cagcaccttt ctggtcacga
    37801 ccgtgcatcg ggttagcacc cggtccgaac ggcgcgccag cacgacgacc gtctggggtg
    37861 ttaccagttt tcttaccata cacaacgtta gaagtgatgg tcagaacaga ctgagtcggg
    37921 atagcgtcac ggtaggtgtg cagtttctga attttcttca tgaaacgttc taccaggtca
    37981 acagccaggt catctacacg cggatcattg ttaccaaact gcgggtattc gccttcgatt
    38041 tcgaagtcga tagcaagacc gtcttcgtca cgaatcggtt taactttcgc atatttgatt
    38101 gcagacaggg agtcagcagc aacggacaga ccagcgatac cacacgccat ggtgcggata
    38161 acgtcacggt cgtgcagcgc catcagagag gcttcgtagc tgtacttgtc gtgcatgtag
    38221 tggatgatgt tcagtgcagt gatgtactgt ttagccagcc agtccatgaa gtgatccatg
    38281 cgctccatca cttcatcgta gttcaggaga tcgcctttga tcggttcaga cttcggacca
    38341 acctgcattt tcagtttttc gtcaacgccg ccgttgattg cgtacagcat ggttttcgcc
    38401 aggtttgcac gcgcaccgaa gaactgcatt tgtttaccaa cgatcatcgg gcttacgcag
    38461 caagcgatag catagtcatc gttgttgaag tccggacgca tcaggtcatc gttctcatac
    38521 tgcagagaag aggtgtcgat ggacacttta gcggcgaatt tcttgaagtt cagcggcagt
    38581 ttttcagacc acagaatggt catgttcggt tccggagacg gacccatggt gtacagggtg
    38641 ttcaggaaac ggaagctgtt tttggtaacc agggtacgac cgtcgaggcc cataccaccg
    38701 atagattcgg ttgcccagat cgggtcgcca gagaacagtt catcgtattc cggagtacgc
    38761 aggaagcgaa ccatacgcag tttcatgacc aggtggtcaa ccatttcctg cgcttcttgt
    38821 tcggtgatct tgccagcttt caggtcacgt tcgatgtaca catccaggaa ggtggaggta
    38881 cgaccgaagg acattgcagc accgttctga gacttaacag cagccaggta gccgaagtaa
    38941 gtccactgga tagcttcctg agcgttggta gccggaccag agatgtcgta gccgtatttc
    39001 gcagccattt ctttcatctg acccagagcg cggtgctgtt cagcgatttc ttcgcgcaga
    39061 cggatagtct gttccaggtt tacgccgttt tccagatcag cctgcagaga agtgaactgt
    39121 gccagtttgt ctttcatcag gtagtcgata ccgtacagcg caacgcgacg gtagtcaccg
    39181 atgatacggc cacggccata tgcatctggc agaccggtca gaacaccaga tttacggcaa
    39241 cgcaggatgt ccggagtgta aacgtcgaac acgccctggt tgtgagtttt acggtattca
    39301 gtgaagattt ttttgatcat cggatccagt tcgcggttgt acgctttgca ggaaccttcg
    39361 atcattttga taccaccgaa cgggataaga gcacgtttca gcggagcttc agtctgcaga
    39421 ccaacgattt tctcaagctg cttgttgatg tagccagcgt cgtgagaggt gatggtggaa
    39481 gcaacagcgg tgtcaaagtc aactggcgcg tgagtgcggt tttccagttt aacgccttcc
    39541 attactttgt cccacagggt ggtggtcgct tcagtagcgc cagccaggaa ggactcgtca
    39601 ccctcgtacg gagtgtagtt tttctgaatg aagtcacgga cgtttacttc attctgccag
    39661 tcacctttgg taaaaccttc ccaggctgtg gctaactttt cattaagctc ggacatgtaa
    39721 cacctacctt cttaagtgga ttttttattt actgcgtact tcgacaacca ttaatggtga
    39781 tcgttttcac gcaggtaaat gacccagtat gtcaacccaa ccaacaaacc accgccgata
    39841 atattaccga tcgtaaccgg aatcaggtta tcagtgatga agttcatcac ggtcaggtga
    39901 gaaaaatttt ccggtgcaga accgacagcg gtccagaatt ccggagatgc gaagtcgcgg
    39961 attacaatac ccatcgggat cataaacatg tttgcgatac tgtgctcaaa accgctggca
    40021 acaaacatcg cgaccggcag caccataatg aacgctttgt ccatcaggct acggccagaa
    40081 taactcatcc atactgccag acataccatc aggtttgcca ggataccaag gcagacggcc
    40141 tcaataaaag tatggtgcac tttgtggtcg gcggtttgta ggacgtttag tccccattga
    40201 ccatttgcgg tcatatactc gccggaaagc cacattaaaa gtacaaacag cagtgcgccg
    40261 accaggttgc caaaatagac atttagccag tttttcgcca actgacccca ggtgatgcgc
    40321 ccactcgcct tagcaacaac aatcaacact gtggaagtaa agagatcggc tccgcagaca
    40381 acacaaagaa tcagccccag agagaagcaa atgccgccaa ccagttttgc catgccgaag
    40441 ggcattgtgc ctgtgccagt ggttgctgtg atatagaaga cgaatgcgat tgagatgaaa
    40501 acaccggcgg taatcgccag atagaaagtc ttaagcggat gtttcgttgc tttatagaca
    40561 cccgcctctt cggccacttt ggccattgca gcaggaagta aaagatcaaa agggttgtca
    40621 gctttcacac taactctctc tttattaagt cggcgacgag atactaacaa agcattatag
    40681 atgagaaatt gatatagatc atatctcgcc tggcttatag gcccgtaact cgcatggttt
    40741 ttatg
//
LOCUS       DAPHDX010000029        40451 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPHDX010000029 DAPHDX010000000
VERSION     DAPHDX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.029"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            295..381
                     /gene="leuL"
                     /locus_tag="RH058_001475"
     CDS             295..381
                     /gene="leuL"
                     /locus_tag="RH058_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV3137079.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            474..2045
                     /gene="leuA"
                     /locus_tag="RH058_001476"
     CDS             474..2045
                     /gene="leuA"
                     /locus_tag="RH058_001476"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV3137080.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2045..3136
                     /gene="leuB"
                     /locus_tag="RH058_001477"
     CDS             2045..3136
                     /gene="leuB"
                     /locus_tag="RH058_001477"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV3137081.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3139..4539
                     /gene="leuC"
                     /locus_tag="RH058_001478"
     CDS             3139..4539
                     /gene="leuC"
                     /locus_tag="RH058_001478"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV3137082.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4550..5155
                     /gene="leuD"
                     /locus_tag="RH058_001479"
     CDS             4550..5155
                     /gene="leuD"
                     /locus_tag="RH058_001479"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV3137083.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5204..6382)
                     /gene="setA"
                     /locus_tag="RH058_001480"
     CDS             complement(5204..6382)
                     /gene="setA"
                     /locus_tag="RH058_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDV3137084.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6484..6615)
                     /gene="sgrT"
                     /locus_tag="RH058_001481"
     CDS             complement(6484..6615)
                     /gene="sgrT"
                     /locus_tag="RH058_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV3137085.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6704..8359
                     /gene="sgrR"
                     /locus_tag="RH058_001482"
     CDS             6704..8359
                     /gene="sgrR"
                     /locus_tag="RH058_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV3137086.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8393..8492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8523..9506
                     /gene="thiB"
                     /locus_tag="RH058_001483"
     CDS             8523..9506
                     /gene="thiB"
                     /locus_tag="RH058_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV3137087.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9482..11092
                     /gene="thiP"
                     /locus_tag="RH058_001484"
     CDS             9482..11092
                     /gene="thiP"
                     /locus_tag="RH058_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV3137088.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11076..11774
                     /gene="thiQ"
                     /locus_tag="RH058_001485"
     CDS             11076..11774
                     /gene="thiQ"
                     /locus_tag="RH058_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV3137089.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11876..12640)
                     /locus_tag="RH058_001486"
     CDS             complement(11876..12640)
                     /locus_tag="RH058_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3137090.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12726..13604)
                     /gene="araC"
                     /locus_tag="RH058_001487"
     CDS             complement(12726..13604)
                     /gene="araC"
                     /locus_tag="RH058_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV3137091.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13943..15643
                     /gene="araB"
                     /locus_tag="RH058_001488"
     CDS             13943..15643
                     /gene="araB"
                     /locus_tag="RH058_001488"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV3137092.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTHPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15654..17156
                     /gene="araA"
                     /locus_tag="RH058_001489"
     CDS             15654..17156
                     /gene="araA"
                     /locus_tag="RH058_001489"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV3137093.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17325..18020
                     /gene="araD"
                     /locus_tag="RH058_001490"
     CDS             17325..18020
                     /gene="araD"
                     /locus_tag="RH058_001490"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV3137094.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18095..20446
                     /gene="polB"
                     /locus_tag="RH058_001491"
     CDS             18095..20446
                     /gene="polB"
                     /locus_tag="RH058_001491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV3137095.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20611..23517
                     /gene="rapA"
                     /locus_tag="RH058_001492"
     CDS             20611..23517
                     /gene="rapA"
                     /locus_tag="RH058_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV3137096.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23529..24188
                     /gene="rluA"
                     /locus_tag="RH058_001493"
     CDS             23529..24188
                     /gene="rluA"
                     /locus_tag="RH058_001493"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV3137097.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24305..25120)
                     /gene="djlA"
                     /locus_tag="RH058_001494"
     CDS             complement(24305..25120)
                     /gene="djlA"
                     /locus_tag="RH058_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV3137098.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25375..27729
                     /gene="lptD"
                     /locus_tag="RH058_001495"
     CDS             25375..27729
                     /gene="lptD"
                     /locus_tag="RH058_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV3137099.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27782..29068
                     /gene="surA"
                     /locus_tag="RH058_001496"
     CDS             27782..29068
                     /gene="surA"
                     /locus_tag="RH058_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV3137100.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29068..30057
                     /gene="pdxA"
                     /locus_tag="RH058_001497"
     CDS             29068..30057
                     /gene="pdxA"
                     /locus_tag="RH058_001497"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV3137101.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30054..30875
                     /gene="rsmA"
                     /locus_tag="RH058_001498"
     CDS             30054..30875
                     /gene="rsmA"
                     /locus_tag="RH058_001498"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV3137102.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30878..31255
                     /gene="apaG"
                     /locus_tag="RH058_001499"
     CDS             30878..31255
                     /gene="apaG"
                     /locus_tag="RH058_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV3137103.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31262..32104
                     /gene="apaH"
                     /locus_tag="RH058_001500"
     CDS             31262..32104
                     /gene="apaH"
                     /locus_tag="RH058_001500"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV3137104.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32182..32661)
                     /gene="folA"
                     /locus_tag="RH058_001501"
     CDS             complement(32182..32661)
                     /gene="folA"
                     /locus_tag="RH058_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV3137105.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32853..34715)
                     /gene="kefC"
                     /locus_tag="RH058_001502"
     CDS             complement(32853..34715)
                     /gene="kefC"
                     /locus_tag="RH058_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV3137106.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34708..35238)
                     /gene="kefF"
                     /locus_tag="RH058_001503"
     CDS             complement(34708..35238)
                     /gene="kefF"
                     /locus_tag="RH058_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV3137107.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35346..36677)
                     /locus_tag="RH058_001504"
     CDS             complement(35346..36677)
                     /locus_tag="RH058_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137108.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLISSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36735..37022)
                     /gene="fixX"
                     /locus_tag="RH058_001505"
     CDS             complement(36735..37022)
                     /gene="fixX"
                     /locus_tag="RH058_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV3137109.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37019..38305)
                     /locus_tag="RH058_001506"
     CDS             complement(37019..38305)
                     /locus_tag="RH058_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3137110.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38356..39297)
                     /locus_tag="RH058_001507"
     CDS             complement(38356..39297)
                     /locus_tag="RH058_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV3137111.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39312..40082)
                     /locus_tag="RH058_001508"
     CDS             complement(39312..40082)
                     /locus_tag="RH058_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV3137112.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPNELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9749 a        11126 c        10623 g         8953 t
ORIGIN      
        1 caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc
       61 cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag
      121 cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgcagca caattagcta
      181 attttacgga tgcagaactc acgctggcgg gacgttttta ttgcgtcagg gttgacatcc
      241 gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact
      301 cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga
      361 gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc
      421 gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc
      481 agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct
      541 tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga
      601 tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcggtgcaa accatcgccc
      661 gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg cgtggagaaa gatatcgacg
      721 tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgccactt
      781 cgccaatgca catcgccacc aagctgcgca gcacgctgga tgaagtgatc gaacgcgcta
      841 tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg
      901 ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg
      961 ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa
     1021 tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata
     1081 cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac
     1141 gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag
     1201 aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc
     1261 accaggagat atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg
     1321 caaacaaagc cattgttggc agcggcgcat tcgctcactc ctccggtatc caccaggatg
     1381 gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc
     1441 aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg
     1501 agatggggta taaagaaagt gaatataatt tagacaattt gtacgatgct ttcctgaagc
     1561 tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta
     1621 agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta
     1681 acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg
     1741 ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata
     1801 acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc
     1861 aggtggatat tgtcgccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg
     1921 atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg
     1981 cagaagtcga aaaagagttg caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg
     2041 tgtgatgtcg aagaattacc atattgccgt attgccgggg gacggtattg gtccggaagt
     2101 gatgacccag gcgctgaaag tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac
     2161 caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc
     2221 tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggtcc
     2281 gaaatgggaa catttaccac cagaccagca accagaacgc ggcgcgctgt tgcctttgcg
     2341 taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc
     2401 attctgtccg ctgcgtgcag acattgccgc taacggcttc gacatcctgt gcgtgcgcga
     2461 actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga
     2521 aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc
     2581 gtttgaatct gctcgtaaac gtcgccacaa agtcacctca atcgacaaag ctaacgtgct
     2641 gcaatcctct attttatggc gtgaaatcgt caacgagatc gccacggaat acccggatgt
     2701 cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca
     2761 gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat
     2821 gatcactggc tcgatgggga tgttgccttc cgccagcctg aacgagcaag gttttggtct
     2881 gtatgaaccg gcaggcggct cagcaccaga tatcgcaggc aaaaacatcg ccaacccgat
     2941 tgcgcaaatt ctgtcgctgg cactgctgct gcgctacagc ctggatgccg atgatgcggc
     3001 ttgcgccatt gaacgcgcca ttaaccgcgc attagaagaa ggcattcgca ccggggattt
     3061 agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt
     3121 ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta
     3181 gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa
     3241 gtgacctcac cgcaggcgtt tgatggtctg cgcgcccacg gtcgcccggt acgtcagccg
     3301 ggcaaaacct tcgccactat ggatcacaac gtctctaccc agaccaaaga cattaatgcc
     3361 tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc
     3421 gtcgagctgt atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa
     3481 cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac
     3541 ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca
     3601 acacaaaccc tgaaacaggg ccgtgcgaag accatgaaaa ttgaagtcca gggcaaagcc
     3661 gcgccgggca ttacagcaaa agatatcgtg ctggcaatta tcggtaaaac tggcagcgca
     3721 ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa ttcgtgattt aagcatggaa
     3781 ggtcgtatga ccctgtgcaa tatggcaatc gaaatgggcg caaaagccgg tctggttgca
     3841 ccggacgaaa ccacctttaa ctatgtcaaa ggccgtttgc atgcgccgaa aggcaaagat
     3901 ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat
     3961 accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatcca
     4021 ggccaggtga tttccgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt
     4081 gaacgcgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg cattccgctg
     4141 accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactcgcg cattgaagat
     4201 ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg
     4261 gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaatc
     4321 tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac
     4381 aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc
     4441 cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct
     4501 gtgaccggac atttcgccga cattcgcaac attaaataag gagcacacca tggcagagaa
     4561 atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc
     4621 aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt
     4681 taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa
     4741 cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc
     4801 ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc
     4861 gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt
     4921 aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt
     4981 cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat
     5041 cgatgccttc cgccgccact gcatgatgaa cggtctggac agtattgggc ttaccttgca
     5101 gcacgacgac gccattgccg cttatgaagc aaaacaacct gcgtttatga attaatcccc
     5161 ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct
     5221 ttgcggttaa aaataatgcg acaacagaaa taaccgcaat tacccagtag acagcaaagt
     5281 gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc ccggtagaaa
     5341 tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc
     5401 atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca
     5461 tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact cctgccgcca
     5521 ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca
     5581 ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca
     5641 atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg ttgcaggtcc
     5701 acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag ccaccttgca
     5761 ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc ataaatgcaa
     5821 tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc gtaaagccgt
     5881 aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga gaaagctgcg
     5941 cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca tattcccgcg
     6001 ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg
     6061 tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg atagccatca
     6121 aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt tttgccaacc
     6181 agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg atccagaaag
     6241 gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg
     6301 ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag
     6361 ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca
     6421 gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc
     6481 aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca tggttaatcg
     6541 ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg
     6601 ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata
     6661 gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct
     6721 gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct
     6781 caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac
     6841 catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg
     6901 tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga
     6961 gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat
     7021 gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta
     7081 ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat
     7141 tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc
     7201 agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc
     7261 aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa
     7321 acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc cgacgccctg
     7381 gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc tgggacaagt
     7441 tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca gccatcccat
     7501 cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa ttcaggcatt
     7561 cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg ttctgccgga
     7621 aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggcg aggaaaaaga
     7681 gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgacagcc gcacccatcg
     7741 cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt
     7801 ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg
     7861 ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa gcctcaccct
     7921 gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct
     7981 ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt ggcatgaagg
     8041 agagatcgag agcgatatct ggcttaatag cgccaacttt acgctgccgc tggatttttc
     8101 gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga tcgactggca
     8161 agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt gccagcaact
     8221 ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc agggacaacg
     8281 cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat cagcgtggtt
     8341 tgcgccgccg gatccatgat tgctggtaga taacaaaatc actacactaa cgccgttctc
     8401 aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa
     8461 cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca
     8521 aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac
     8581 ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccaaaga
     8641 tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg ctggaagatg
     8701 gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc
     8761 tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt gccaaaagcg
     8821 gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtcccgt
     8881 ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa
     8941 gcctgaaaga attggttgag agcgatcaaa actggcgggt gatttatcag gatccgcgca
     9001 ccagtacgcc ggggctgggc ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc
     9061 cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag
     9121 cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg
     9181 cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc
     9241 actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc
     9301 aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca accggtaact
     9361 ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg
     9421 ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca tggattagcg
     9481 aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc
     9541 cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca
     9601 gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt
     9661 ctggcaggcg tttctctcgg cgctactatc tgtcgtaccc gcgatattcc tcgcccgcgc
     9721 cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt
     9781 gatcctcccg gtgctagttg ctgttttcgg cattcttagc gtctatggtc gtcagggctg
     9841 gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca
     9901 aggcattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct tattactcca
     9961 ggcactggaa aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg
    10021 ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc
    10081 cgcgcttatc tttatgctct gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg
    10141 tccgcaggca actactatcg agctggctat ttatcaggcg ctgagttacg actacgatcc
    10201 tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt
    10261 gagtcagcga ctaagtaagg ccattgcgcc cggtaccacg ctgctgcaag gctggcgcga
    10321 tccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc tggcgctgct
    10381 gctgttgctg ccaccgttgc tggcggtgat cgtcgatggc gtaaatcgcc agttgccgga
    10441 agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc
    10501 ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg
    10561 ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt tgatcctcgc
    10621 catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca ctatcggcct
    10681 gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc
    10741 gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca
    10801 gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg
    10861 tccactggcg caggcgctgg cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt
    10921 ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg
    10981 ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac tctgttttct
    11041 gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct
    11101 ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg
    11161 caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc
    11221 tgacgccagc cagcggttcg ctgactatcg acggcgttga tcacaccacg acgccgccgt
    11281 cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac ctgacggtcg
    11341 cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg cagcaggaga
    11401 aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg ttaccgggcg
    11461 agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc
    11521 cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt caggagatgc
    11581 tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca
    11641 gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac gggcgcatcg
    11701 cctggcaggg taagaccaat gagttgttga gcggtaaggc gagtgcttcg gcactattgg
    11761 ggattacggg ttagtatgca ctgtcggatg cggcgtgaac gccttatccg acctacgggt
    11821 agtgcgtttt gtaggcctga taagacgcgg caagcgtcgc atcaggcaac ccgccctaaa
    11881 ccccaaccac tttacgcaaa atatcgatat acaccggcat caacgggtgg cgaattaaca
    11941 ccaccagcgc caccacgccg atggcagaaa tcaacggcgt cagccacaac aaacgaccgc
    12001 gggacaaata atgactcaaa cggtcagtcg ctttaccgct gcgccataac cgccagcaca
    12061 gccagccacc aacccacaaa aataccgctg ttgccagcag caaccattta aactcaccgc
    12121 tctgcattcc ggcaggaata tcgatcgccg cgcccgccag aatccccggc aggaagtaaa
    12181 acggcggcca taacaggcag ccaataatat ttggcgtgat aaattttgcc accggcagat
    12241 ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg gccaacaaag cgaccgacca
    12301 gaatggtgaa catgctgtgt tgatgcaacg catgttcagt tttatcgagt aatgctttgt
    12361 ttttcttcag aaatgaccag cgatgcaacg gctttttaaa acgccagccc agccagaagg
    12421 agatccagtc gcccagcaga caaccgacga tccctgccag ccaggcgtgc cagaaactta
    12481 actcgccgct gccaatcagc gctcccagcc ccgccatcag caccgtaccg ggtagaatca
    12541 aaccgaccag cgccagcgac tccagaaagg ccaccaacac caccgccatc aatgaataca
    12601 cggtagattg ggtaataaag tgttccagca atgcttgcat aatgtgcctg tcaaatggac
    12661 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
    12721 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
    12781 tcgctcgggc tggccccggt gcatttttta aatactcgcg agaaatagag ttgatcgtca
    12841 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
    12901 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
    12961 aaaagatgtg acagacgcga cggtgacaag caaacatgct gtgcgacgct ggcgatatca
    13021 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
    13081 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
    13141 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
    13201 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa acccgtattg
    13261 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
    13321 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
    13381 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
    13441 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
    13501 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcgtta
    13561 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
    13621 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
    13681 tactggctct tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa
    13741 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
    13801 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
    13861 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
    13921 tttttttgga tggagtgaaa cgatggcgat tgcaattggc ctcgattttg gcagtgattc
    13981 tgtgcgagct ttggcggtgg actgcgccag cggtgaagag atcgccacca gcgtagagtg
    14041 gtatccccgt tggcaaaaag ggcaattttg tgatgccccg aataaccagt tccgtcatca
    14101 tccgcgtgac tacattgagt caatggaagc ggcactgaaa accgtgcttg cagagcttag
    14161 cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac agtaccggct cgacgcccgc
    14221 accgattgat gccgacggta acgtgctggc gctgcgcccg gagtttgccg aaaacccaaa
    14281 cgcgatgttc gtattgtgga aagaccacac tgcggttgaa gaagcggaag agattacccg
    14341 tttgtgccac gcgccgggca acgttgacta ctcccgctac attggtggta tttattccag
    14401 cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg tggcgcaatc
    14461 tgccgcatcg tggattgagc tgtgcgactg ggtgccagct ctgctttccg gtaccaccca
    14521 cccgcaggat attcgtcgcg gacgttgcag cgccgggcat aaatctctgt ggcacgaaag
    14581 ctggggcggc ttgccgccag ccagtttctt tgatgagctg gacccgatcc tcaatcgcca
    14641 tttgccttcc ccgctgttca ctgacacctg gactgccgat attccggtgg gcaccttatg
    14701 cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg gcggcgcgtt
    14761 tgactgccat atgggcgcag ttggcgcagg cgcacagcct aacgcactgg taaaagttat
    14821 cggtacttcc acctgcgaca ttctgattgc cgacaaacag agcgttggcg agcgggcagt
    14881 taaaggtatt tgcggtcagg ttgatggcag cgtggtgcct ggatttatcg gtctggaagc
    14941 aggccaatcg gcgtttggtg atatctacgc ctggttcggt cgcgtactca gctggccgct
    15001 ggaacagctt gccgcccagc atccggaact gaaagcgcaa atcaacgcca gccagaaaca
    15061 actgcttccg gcgctgaccg aagcatgggc caaaaatccg tctctggatc acctgccggt
    15121 ggtgctcgac tggtttaacg gtcgtcgcac accgaacgct aaccaacgcc tgaaaggggt
    15181 gattaccgat cttaacctcg ctaccgacgc tccgctgctg ttcggcggtt tgattgctgc
    15241 caccgccttt ggcgcacgcg caatcatgga gtgctttacc gatcagggga tcgccgtcaa
    15301 taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg
    15361 cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc
    15421 ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa
    15481 aatggccagt gcggtagaga aaaccctgca accgcgcagc gaacaggcac aacgctttga
    15541 acagctttat cgccgctatc agcaatgggc gatgagcgcc gaacaacact atcttccaac
    15601 ttccgccccg gcacaggctg cccaggccgt tgcgactcta taaggacacg ataatgacga
    15661 tttttgataa ttatgaagtg tggtttgtca ttggcagcca gcatctgtat ggcccggaaa
    15721 ccctgcgtca ggtcacccaa catgccgagc acgttgttaa tgcgctgaat acggaagcga
    15781 aactgccctg caaactggtg ttgaaaccgc tgggcaccac gccggatgaa atcaccgcta
    15841 tttgccgcga cgcgaattac gacgatcgtt gcgctggtct ggtggtgtgg ctgcacactt
    15901 tctccccggc caaaatgtgg atcaacggcc tgaccatgct caacaaaccg ttgctgcaat
    15961 tccacaccca gtttaacgcg gcgctgccgt gggacagtat cgatatggac tttatgaacc
    16021 tgaaccagac tgcacatggc ggtcgcgagt tcggcttcat tggcgcgcgt atgcgtcagc
    16081 aacatgccgt ggttaccggt cactggcagg ataaacaagc ccatgagcgt atcggctcct
    16141 ggatgcgcca ggcggtctct aaacaggata cccgtcatct gaaagtctgc cgttttggcg
    16201 ataacatgcg tgaagtagcg gtcaccgatg gtgataaagt tgccgcacag atcaagttcg
    16261 gtttctccgt caatacctgg gcggttggcg atctggtgca ggtggtgaac tccatcagcg
    16321 acggcgatgt taacgcgctg gtcgatgagt acgaaagctg ctacaccatg acgcctgcca
    16381 cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc ggcgcgtatt gagctgggga
    16441 tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac caccaccttt gaagatttgc
    16501 acggtctgaa acagcttcca ggtctggccg tacagcgtct gatgcagcag ggttacggct
    16561 ttgcgggcga aggcgactgg aaaaccgccg ccctgcttcg catcatgaag gtgatgtcaa
    16621 ccggtctgca gggcggcacc tcctttatgg aggactacac ctatcacttc gagaaaggta
    16681 atgacctggt gctcggctcc catatgctgg aagtctgccc gtcgattgcc gtagaagaga
    16741 aaccgatcct cgacgttcag catctcggta ttggtggtaa ggacgatcct gcccgactga
    16801 tcttcaatac ccaaaccggt ccagcgattg tcgccagcct gattgatctc ggcgatcgtt
    16861 accgtctgct ggttaactgt atcgacacgg tgaaaacacc gcactccctg ccgaaactgc
    16921 cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga
    16981 tccttgctgg tggcgcgcac cataccgtct tcagccatgc gctgaacctc aacgatatgc
    17041 gccagttcgc cgagatgcac gacattgaaa tcacggtgat tgataacgac acccgcctgc
    17101 cagcgtttaa agacgcactg cgctggaacg aagtgtatta cggatttcgt cgctaagtag
    17161 ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt cttatctggc ctacacgctg
    17221 cgattttgta ggccggataa gcaaagcgca tccggcattg ttttccccct cgcccctttg
    17281 gggagagggc cggggtgagg ggaaacgacc tctcaggagt caacatgtta gaagatctca
    17341 aacgccaggt attagaagcc aacctggcgc tgccaaaaca caacctggtc acgctcacat
    17401 ggggcaacgt cagcgccgtt gatcgcgagc gcggcgtctt tgtgatcaaa ccttccggcg
    17461 tcgattacag cgtcatgacc gctgacgata tggtcgtggt tagcatcgaa accggtgaag
    17521 tggttgaagg tacgaaaaag ccctcctccg acacgccaac tcaccggctg ctctatcagg
    17581 cattcccctc cattggcggc attgtgcata cgcactcgcg ccacgccacc atctgggcgc
    17641 agtcgggtca gtcgattcca gcaatcggca ccacccacgc cgactatttc tacggcacca
    17701 ttccctgcac ccgcaaaatg accgacgcag aaatcaacgg cgaatatgag tgggaaaccg
    17761 gtaacgtcat cgtagaaacc tttgaaaaac agggtatcga tgcagcgcaa atgcccggcg
    17821 ttctggtcca ttcccacggc ccgtttgcat ggggcaaaaa tgccgaagat gcggtgcata
    17881 acgccatcgt gctggaagag gtcgcttata tggggatatt ctgccgtcag ttagcgccgc
    17941 agttaccgga tatgcagcaa acgctgctgg ataaacacta tctgcgtaag catggcgcga
    18001 aggcatatta cgggcagtaa tgactgtata aaaccacagc caatcaaacg aaaccaggct
    18061 atactcaagc ctggtttttt gatggatttt cagcgtggcg caggcaggtt ttatcttaac
    18121 ccgacactgg cgggacaccc cgcaagggac agaagtctcc ttctggctgg cgacggacaa
    18181 cgggccgttg caggttacgc ttgcaccgca agagtccgtg gcgtttattc ccgccgatca
    18241 ggttccccgc gctcagcata ttttgcaggg tgaacaaggc tttcgcctga caccgctggc
    18301 gttaaaggat tttcaccgcc agccggtgta tggcctttac tgtcgcgccc atcgccaatt
    18361 gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc gtctacgagg ccgatgtgcg
    18421 cccgccagaa cgctatctga tggagcggtt tatcacctca ccggtgtggg tcgagggtga
    18481 tatgcacaat ggcactatcg ttaatgcccg tctgaaaccg catcccgact atcgtccgcc
    18541 gctcaagtgg gtttctatag atattgaaac cacccgccac ggtgagctgt actgcatcgg
    18601 cctggaaggc tgcgggcagc gcatcgttta tatgctgggg ccggagaatg gcgacgcctc
    18661 ctcgcttgat ttcgaactgg aatacgtcgc cagccgcccg ctgctgctgg aaaaactcaa
    18721 cgcctggttt gcaaaccacg atcccgatgt gatcatcggc tggaacgtgg tgcagttcga
    18781 tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtctt ccgctgcgtc ttgggcgcga
    18841 taatagcgag ctggagtggc gcgagcacgg ctttaaaaac ggcgtctttt ttgcccaggc
    18901 taaaggtcgg ctaattatcg acggtatcga ggcgctgaaa tccgcgttct ggaatttctc
    18961 ttcattctcg ctggaaactg tcgctcagga gctattaggc gaaggaaaat ctatcgataa
    19021 cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc gaagataaac ctgcgctggc
    19081 aacttataac ctgaaagatt gcgagctggt gacgcagatc ttccacaaaa ctgaaatcat
    19141 gccattttta ctcgaacgag caacggtgaa cggcctgccg gtggatcggc acggcggttc
    19201 ggtggcagcg tttggtcatc tctattttcc gcgaatgcat cgcgctggtt atgtcgcgcc
    19261 taatctcggc gaagtgccac cgcacgccag ccctggcggc tacgtgatgg attcacggcc
    19321 agggctttat gattcagtgc tggtgctgga ctataaaagc ctgtacccgt cgatcatccg
    19381 cacctttctg attgatcccg tcgggctggt tgaaggcatg gcgcaacctg atccagagca
    19441 cagtaccgaa ggttttctcg atgcctggtt ctcacgggag aaacattgcc tgccggagat
    19501 tgtgactaac atctggcacg ggcgcgatga agccaaacgt cagggtaata agccgctttc
    19561 gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg ctcggcacca ccgcctgccg
    19621 cttcttcgat ccgcgcctgg catcgtcgat caccatgcgt ggtcatcaga tcatgcggca
    19681 gaccaaagca ttgattgaag cgcagggcta cgacgttatc tacggagata ccgactcgac
    19741 ttttgtctgg ctaaaaggtg cacattcgga agaagaggcg gcgaaaatcg gtcgtgtact
    19801 ggtgcagcac gttaacgcct ggtgggcgga aacgctgcaa aaacaacggc tgaccagcgc
    19861 attagaactg gagtatgaaa ctcatttctg ccgttttctg atgccaacca ttcgcggagc
    19921 cgataccggc agtaaaaagc gctacgccgg gctgattcag gagggcgata aacagcggat
    19981 ggtgtttaaa gggctggaaa ccgtgcgcac cgactggacg ccgctggccc agcagtttca
    20041 gcaggagcta tacctgcgca tcttccgcaa cgagccatat caggaatatg tacgcgaaac
    20101 catcgacaaa ctgatggcgg gtgaactgga tgcgcgactg gtttaccgta aacgccttcg
    20161 ccgtccgctg agcgagtatc agcgtaatgt gccgcctcat gtacgcgccg ctcgccttgc
    20221 cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat cagaatcgcg gcaccattaa
    20281 gtacgtatgg accaccaacg gcccggagcc gctggactac caacgttcac cactggatta
    20341 cgaacactat ctgacccgcc agctacaacc cgtggcggag ggaatactcc cttttattga
    20401 ggataatttt gctacactta tgaccgggca acttgggcta ttttgagcaa aaaaaagagt
    20461 tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca tagcgccctt
    20521 tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg tggttattgc
    20581 ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca acgctggatc
    20641 agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc gcgaactgtc
    20701 actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga ttcccccgtg
    20761 acccgcgtga tgttcaaccc tggtgatact attaccagcc atgacggctg gcagatgcaa
    20821 gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg cctggatact
    20881 gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa
    20941 ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc gctgcgttat
    21001 cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct gcgcggtcag
    21061 cgtaccagcc tgattccgca tcagctcaac atcgctcatg atgttggtcg ccgccacgcg
    21121 ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc cgggatgatc
    21181 ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc taattatcgt cccggaaacc
    21241 ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt tgcgctattt
    21301 gatgatgagc gttatgccga agctcagcac gatgcttaca acccgtttga caccgaacag
    21361 ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac agcgcctgga acatctctgt
    21421 gaagccgaat gggacctgct ggtggtcgat gaagcgcatc acctggtgtg gagcgaagat
    21481 gcgccaagcc gtgaatatca ggccattgaa caactggcag agcacgtgcc gggcgttctg
    21541 ctgctgaccg cgaccccgga acagctgggg atggaaagcc acttcgcccg tctgcgtctg
    21601 ctggacccga accgtttcca cgattttgcg cagttcgttg aagagcagaa aaattatcgt
    21661 ccggttgcgg acgccgttgc catgctgctg gcaggtaaca aactgagcaa tgacgaactg
    21721 aacatgctcg gcgagatgat cggcgagcag gatatcgagc cgctgttgca ggcagcaaac
    21781 agcgacagcg aagatgccca gagcgcccgt caggagctgg tttcgatgct gatggatcgc
    21841 cacggcacca gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg attcccgaaa
    21901 cgcgagctgc acaccattaa actgccgcta ccgacgcagt atcagacggc tattaaagtc
    21961 tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg ctcgcgatat gctctacccg
    22021 gagcgtattt atcaggaatt tgaaggtgat aacgccacct ggtggaactt cgatccgcgc
    22081 gttgagtggc tgatgggcta cctgaccagc catcgctctc agaaagtgct ggtgatctgc
    22141 gccaaagctg ccactgcgct gcaactggag caggtactgc gcgaacgtga aggtattcgc
    22201 gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg accgcgctgc cgcctggttt
    22261 gccgaagaag acaccggcgc acaggtactg ctgtgctcag aaatcggttc tgaaggacgt
    22321 aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc ggatctactg
    22381 gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc acgatattca gatccatgtg
    22441 ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct ggtatcacga aggtctggat
    22501 gcatttgagc acacctgccc gaccggacgc actatttacg atagcgtata caacgatctg
    22561 attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat caaaaactgc
    22621 cgcgagcaac atgaagcgct gaaagcacag ctggaacagg gtcgtgaccg cctgctggaa
    22681 atccactcca acggtggcga aaaagcccag gcactggcag aaagcattga agagcaggat
    22741 gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg tatcaatcag
    22801 gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct ggtgccggac
    22861 ttccctggcc tgtcggaaga tggcatcacc atcacctttg atcgtgaagt ggcgctggcg
    22921 cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg tctggatctg
    22981 attctttctg gcgataccgg tagcagcacg atttcactgt taaaaaacaa agcgttgccg
    23041 gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc gaagcagttg
    23101 cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa aaacggcaac
    23161 aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc ggttaaccgt
    23221 cacaccggca gcaaactggt taacgccgtg cagcaggatg ttcacgctat ccttcaactg
    23281 ggtgaagcgc agatcgagaa atctgcccgt gcattgattg atgcagcgcg taacgaagcc
    23341 gacgaaaaac tgtctgccga gctgtctcgt ctggaagctc tgcgtgcagt gaacccgaac
    23401 attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat ggaaagcctg
    23461 gatcaggcag gttggcgtct ggatgccctg cgtttgatcg ttgtaacgca tcagtaacgg
    23521 agccgaaaat ggggatggaa aactacaatc caccgcagga accctggttg gttatcctgt
    23581 atcaggatga ccatattatg gtggtcaaca agccgagcgg tttgttgtca gtgccgggtc
    23641 gtctggaaga gcacaaagac agcgtgatga cgcgcattca gcgtgattat ccgcaggcag
    23701 aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg ctgaccaaag
    23761 ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gccgaaaaag cagtatgtgg
    23821 cccgcgtctg ggggcatcca tcccccgcag aaggtctggt ggatctgccg ctgatttgcg
    23881 actggccaaa ccgcccgaaa cagaaagtct gttacgaaac gggtaaacct gcgcagacgg
    23941 aatatgaagt ggtggagtat gcggcggata acacggcaag agtggtgtta aaaccgatta
    24001 ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct ggggcatccg attctcggcg
    24061 atcgttttta tgcatcacca gaagcgagag cgatggcacc gcgtttgttg ctacatgcag
    24121 agatgttgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa gcgccagcgg
    24181 atttttaatc gtcacgctgg cgcagttcgg taggcggata aggcgttcac gccgcatccg
    24241 gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc ctacagttta
    24301 aaacttattt aaaccctttc tgctgcttta tcagctcata tgcctgctga atttcctgcg
    24361 ctttctgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc agcttatcgg
    24421 gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca tcatccgtcg
    24481 gcttcacgcc cagcacatta caggcatctt ccagcgttgg gccacgctgc gcttgctgcc
    24541 agttaccacc gccagtttgc tgctgataac cgccgccaaa ctgtgcaccg ccctgcatca
    24601 tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca atgacataca
    24661 gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga atctggatct
    24721 ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac tggcgcatct
    24781 tttcgcgcag cgggtaattg tctgattttc ccacccggaa cgcattttgc gccgcagtac
    24841 gggaagcgcc atgaagattc attcggtcca tcaactggct ggcgatatga atatcagcct
    24901 ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg gtggcaaaaa
    24961 acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta cgggctttat
    25021 caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg cccatcagta
    25081 aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca aatcgacaca
    25141 cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc ggctgcggtc
    25201 aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg ccgtgcctaa
    25261 caccactgac gctattcgga caggattaac actagcgtag agatgacgag tacgttagtc
    25321 tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca acgtatgaaa
    25381 aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag tcaacaggga
    25441 ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga ccgtcctctg
    25501 gtacagggcg ataccaatga cttacccgtg actatcaatg ctgaccacgc gaaaggggac
    25561 tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa cagccgtctg
    25621 caggccgacg aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga gccggtacgt
    25681 accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct caaagggccg
    25741 aaaggctggg cgaatctgaa caccaaagat accaacgtct gggaaggtga ttaccagatg
    25801 gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga aaaccgctat
    25861 accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac ctggagcgtg
    25921 gtaggtagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg gaacgcccgc
    25981 tttaaggtgg gtccggtacc gatcttttat agcccctatt tgcagttgcc ggtgggtgac
    26041 aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa ctactttgag
    26101 ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat cacgccgcat
    26161 tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat tccgctacct ctcccaggcg
    26221 ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga agatgaacac
    26281 ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg ggtcatggat
    26341 caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta cttcaatgat
    26401 ttcgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt cagcgttggc
    26461 tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt tttcagcgaa
    26521 cagaacacca gtagctactc ggcagagccg cagttagacg ttaattacta ccagaatgat
    26581 gttggtccgt ttgatacgcg tatttacggc caggcagtgc actttgttaa caccagagac
    26641 gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc gctctctaat
    26701 aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca gcaaactaat
    26761 cttgactggt ataactccag aaacacgacc aagctggacg aatccgttaa ccgcgtaatg
    26821 ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat gctggctccg
    26881 ggttataccc aaacgctgga accgcgcgcg cagtatttgt acgtgccgta tcgcgatcag
    26941 agcgacatct ataactacga ctcatctctg ctgcaatctg actactctgg cctgttccgg
    27001 gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac gaccggtgtc
    27061 acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt tggtcaaatc
    27121 tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa tgacgacaaa
    27181 acgggctcac tggtgtgggc aggcgatact tactggcgta tctccgagcg ttggggattg
    27241 cgtggcggga ttcagtacga tacacgtctg gataacgtag cgaccagtaa ctccagcatt
    27301 gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc cagcccggaa
    27361 tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa gaatggtatt
    27421 tcgcaggtag gtgctgtcgc cagctggcca attgccgatc gttggtccat tgttggggcc
    27481 tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt gcaatacagc
    27541 tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg ggataacgat
    27601 aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg cggcctgagc
    27661 tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc gtatcaaaac
    27721 actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa atggaaaaag
    27781 tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc
    27841 tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag
    27901 cgacgttgat ggattaatgc agtcggtaaa actgaacgct gctcaggcaa ggcagcaact
    27961 tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg atcaaatcat
    28021 cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg atcaggcgat
    28081 tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc gtctggctta
    28141 cgatggactg aactacaaca cctatcgtaa ccagatccgc aaagagatga ttatctctga
    28201 agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct
    28261 ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga gccacatcct
    28321 gatcccgctg ccggaaaacc cgacctctga tcaggtgaac gaagcggaaa gccaggcgcg
    28381 cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga ttgctcattc
    28441 tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc aggagttacc
    28501 cgggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg gcccgattcg
    28561 ttccggcgtt ggcttccata ttctgaaagt taacgacctg cgcggcgaaa gcaaaaatat
    28621 ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga tcatgactga
    28681 cgaacaggcc cgtgtgaaac tggaacagat tgctgctgat atcaagagtg gtaaaacgac
    28741 ttttgctgcc gcagcgaaag agttctcgca ggatccaggt tctgctaacc agggcggcga
    28801 tctcggttgg gcaacagcgg acatttttga tccggctttc cgtgacgccc tgacccgcct
    28861 gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt taatcgaact
    28921 gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg cataccgcat
    28981 gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac aacgtgccag
    29041 cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt gatcactccc
    29101 ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg tgagtggccg
    29161 gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc gatgctcggt
    29221 ttgccgctca ccctccgcac ttattccccc aactctcctg cacaaccgca aactgcgggc
    29281 acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca gttagcggtt
    29341 gaaaatgggc attatgtggt ggaaacgctg gcgcgagcgt gcgatggctg tctgaacggt
    29401 gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga cgcaggcatt
    29461 ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa agtggtgatg
    29521 atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat
    29581 atcgcagatg ctatcacccc tgcgcttttg cacgaagtga ttgctatttt gcatcacgat
    29641 ttgcggacca aatttggtat tgccgaaccg cgcattctgg tctgcgggct gaatccacac
    29701 gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc gctgctcgac
    29761 gagttgcggg cgcaggggat gaaacttaac gggccgctgc ctgccgatac cctgtttcag
    29821 ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt accacgatca gggtcttccc
    29881 gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct gccctttatt
    29941 cgcacatcag tggaccacgg caccgcgctt gaactggcgg gacgtggcga agccgatgtc
    30001 ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac ccaatgaata
    30061 atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc
    30121 agttcgtgat cgacagcatt gtttctgcca ttaacccgca aaagggccag gcaatggtcg
    30181 aaatcggccc cggtctggcg gcattgaccg agccggtcgg cgaacgtctg gaccagctga
    30241 cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca ttcttaggcc
    30301 cgaaactgac gatttatcag caggatgcga tgacctttaa ctttggcgaa ctggccgaga
    30361 aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc acgccgttga
    30421 tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg ttgcaaaaag
    30481 aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc gtatggtcga ttaagcgtca
    30541 tggcgcaata ctattgcaac gtgatcccag tgctggaagt accgccgtca gcctttacac
    30601 caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg atgcctcacc
    30661 cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga agcctttaac cagcgtcgta
    30721 aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg ggaatgggga
    30781 tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag atggcgaact
    30841 atctggcgga gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc cccgagtgtg
    30901 tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg aacgttacgt
    30961 ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg
    31021 ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg aaggagtggt
    31081 tggcgtccag ccacttatcg cgcctggcga agagtaccag tacaccagcg gtgcaatcat
    31141 tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc
    31201 tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc attaaaagaa
    31261 tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga tcgcattgct
    31321 gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggcg atctggtcgc
    31381 gcgcgggccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg
    31441 tctggtgctg ggcaatcacg atctgcatct gctggcggta tttgccggga tcagccgcaa
    31501 taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg agctgcttaa
    31561 ctggctgcgt cgccagcctt tgctgcaaat cgacgaagag aaaaagttgg tgatggccca
    31621 cgcagggatc acgccgcagt gggatctaca gaccgccaaa gagtgcgcgc gcgatgtaga
    31681 agcggtgcta tcgagtgact cctatccctt ctttctcgac gctatgtacg gcgatatgcc
    31741 aaataactgg tcaccggaat tgcgggggct gggaagactg cgttttatca ccaacgcctt
    31801 tacccgtatg cgtttttgct ttccaaacgg tcaactggat atgtacagca aagaatcgcc
    31861 ggaagaagcc cctgccccac tgaaaccgtg gtttgcgatt cctggccctg tcgctgaaga
    31921 atacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc cggaaggtat
    31981 ttacgcgctg gataccggct gctgctgggg tgggtcatta acctgcctgc gctgggaaga
    32041 taagcagtat tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag cggcggcgtc
    32101 ttaaacacag cctgatatag gaaggccgga taagacgcga ccggcgtcgc atccggcact
    32161 agccgtaaat tctatacaaa attaccgccg ctccagaatc tcaaagcaat agctgtgaga
    32221 gttctgcgca tcggcgtcgt ggaattcgct gaataccgat tcccagtcat ccggctcgta
    32281 atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat acagtttttg
    32341 cgcttttggc aggaactgct cataaacgcg accgccgcca atcaccatga tttctggtac
    32401 gtcaccacac gccgcgatgg cttcatccac cgacttcacc cacgttacgc gatcgtccgt
    32461 acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac cgattgattc
    32521 ccaggtatgg cggcccataa tcacgggttt atttaaggtg ttgcgtttaa accaggcgag
    32581 atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacgcgat ctaccgctaa
    32641 cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc cactatacgt
    32701 aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt tatattctgc
    32761 tggcgagtcc acgctctctc cctggactcg ccgcattaca atgaaacaaa aacaaacagt
    32821 tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt ttccggttca
    32881 tccgccatgt tgccggtatg tttaccttct tcggttccct gccagccatg tcgttgaatt
    32941 aatgacagat gttcgcggtc ctcggtaatg atctcactta acatcgcgct ggtgcgttta
    33001 tagaccgccg cgcgggcttt ggtatcattc tcaaccattg ccatctcttc caccatctga
    33061 atattaaagc ggcggaacac atcggcacgt tctcgcgctt catacggccc cagacctaaa
    33121 ctttccagtg ccagacgccc ggttttcagc gcaccttcga aggtttcacg ctccggcttt
    33181 tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg ggcaataatc
    33241 tgcaaatgcg ggaaatgttc tttcaccatc tctgtcagtt gcaggttggt ttgcggatcg
    33301 tcgatggcgt taatcagcac ttccgctttc gccgctccgg cagattccag taaatccatc
    33361 cgcgtggcat cgccataaaa cactttcata ccaaatttac gcaaggtttc gatatggtcc
    33421 ggatcgtgat cgagtaccac cattttcacc ccgctggaga gcagtaaacg tccggtaatc
    33481 tgcccaaaac gaccgaatcc ggcgataatc acgcgcggct gttcttcgtc gatctcatcg
    33541 gcttcacgcg cttcctcagt agaagattgc tcaaggcgat tgaggatcac cagcagaatc
    33601 ggcgttgctg ccatcgacag cgccaccgcc agggtcagcg atttcgccca ctccggctcc
    33661 agcacattcg ccatctgcgc cgcgccaaat accacaaagg caaactcact gccctgccct
    33721 aacaacaccg caaaccaacg acgctgttta tttggcactt gcaacggtcg ggcaatcagc
    33781 cacagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat gcgcaatggg
    33841 ttttcaagca gcgtgccaaa gtctatcgac atgccaacac cgatgaaaaa cagccccaac
    33901 agcaaacctt taaatggttc gatatcgctc tccagcgcat gacggtattc cgagcttgcc
    33961 agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc ttccagcagc
    34021 aaaccaaagc caaacacgag gaataacgcc acggcactaa acacttcccg caagccagag
    34081 cgggctacaa aacgcagcgc cggacgcgtg acatagcgcc ccagcaatac caccagcacc
    34141 agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt cgaggcactg
    34201 ctcgttgcca gtagcggaat catcgccacc agcgggatcg ccgcgatatc ctggaacagc
    34261 agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttc attcatcgcc
    34321 tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc cgcgacctgc
    34381 cagcgcagcc caagtaacat gcagaacagc cccagcaggc cgccgcaaat caccatctgc
    34441 aatgcgccac cgccgaacac tgccgcacgc agcttccaca gcctttgtgg atcgagttcg
    34501 aggccgataa taaacagcat cagcaccacg ccaatctcgg caaagtgcag aatagattcg
    34561 gcatcggtca ccagtcgcag cccccacggg ccaataatgc agccggcgat caggtagcca
    34621 agtaccgatc ccagaccaag acgtaccgca atgggtacaa tcagcgctgc cgaaccgaga
    34681 taaatcagcg cctgaatcag cgtatggcta tccatgatgg gcctcctgcc attccagcag
    34741 acgttgctta tagtgacgcg cctgcccttc gagggtttcg tcgtcacaaa taaaggtgca
    34801 gtgcatggca aacggtggca gccagttcag cccgcagtag attgccgtcg cctgtagcgg
    34861 ctgcgacagc acatcaaagc ccggatgcgc accaatttca aaatggcttt ccccgccgcc
    34921 ggtcgtcacc gcccacagca aatgtttgcc atgcagcgcc gtgccgccgt gaccgtaagc
    34981 ccagccgtgc gagaaaactt tatcgatcca aagtttgagg agcggtggaa tgctgtacca
    35041 ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct gctcggcggc
    35101 aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga cgccttccag
    35161 cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat acggatgcgc
    35221 ataaattata agaatcatag gtagcccgcg tgattacgcc tttgttttcc cacaaagtgt
    35281 agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag agaaatctga
    35341 acaatttacc ccattctctg cccgcggatc gtcatattgc tggtttgcgc cagtgacatc
    35401 cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga gatacccgcc
    35461 cccattagca tcgtgttact gataccgtaa ttattgataa agatcggcag tgcccaggtc
    35521 gaaacaatgg tgccaatacg gcttaaggac ataatcacgc ccacggcaga ggcgcggata
    35581 tctgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc gccagagaaa
    35641 aaggcataca ccgcaaaggc catcactacc agccagatcc ccatatccgg gatcagcccc
    35701 aaaaccgcca gcgccagcgt catcatggca aagctgctaa tcaacaatgg acgccgtccg
    35761 gcagtgttta gccacagcat cggcggaata cagccgagca taaagaacag gctaatcacc
    35821 acgttcccca gtgccgcgtt tttgccaacc cccaatccca acaaaccaac gatttgcggg
    35881 ccaaaggtgt aaatggcgaa cattgggatc acctggcagg tccagatggc ggcaacaaac
    35941 agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc
    36001 tcttcatcga aagccaccgg ttcgccaaac agttttatca tcatctcttc gcactctttt
    36061 actcgccctt tgcgtaataa ccagcgggga gattcaggca gttcgaatcg accaatcaaa
    36121 atcaacaaac aggggatcgc cgcgctaccc agcatccagc gccagccgcc ttccacatca
    36181 taaagccagt agccgaccag atcggcgcag gtcgcgccga cataccacat ggcggcgata
    36241 aagctgatgg aaaacgcccg ctgacgggta ctggagaact cggtgatcat tgaggtggcg
    36301 atgggataat ctgcaccgat gacaatgccg ataagtaccc gcatcaccaa cagttcgaca
    36361 ggggatgaga caaacatcgt cgccaccgat atcacgccga tggcgatgat atcaatgagg
    36421 aacattttgc gccgtccgac tttatcggag atatagccaa acagcgatgt gccaacgaac
    36481 agcccggcga gcgttcccgc acccagcaag ccaatccagt cagcgtccag tttcagcgcc
    36541 ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc cagaaacgga
    36601 ccaccacttc cccacagcaa aatgcggcgg tgaatagagg agaatttgag atcgtcaaag
    36661 tttctggacg gttgcatggg ttattcctgt tttttgcaat gagagtcagt gcggcgttac
    36721 gtatcaaacc aacatcagcc gtaacggaac tccacaccaa aggtgccgcg cgggtattcc
    36781 cactgttcca gcgccgaccc cagccccaga atgcgacagg tgccgcactc cagacatccg
    36841 gcgtaatcga agcgcacact gccgtcatcc tgcttcttgt acagacctgc ggggcacgct
    36901 ttcaccagca gctccagcac ctgtttatca gcatcggcct tcacaacaat gtgcggatgc
    36961 tcttcatcga cattgaattt attgacgccc agtttgacgt ccacattgac gggagaagtc
    37021 ataaaacggt cactcctttc atgccatcct tgattagatt gatgaagccc actttcttac
    37081 cgtggcggag gattttcttg cgcatcagtt ctggcgcact gccgtcaatg gtgaacaggt
    37141 cacgcgccac gcccaccgcc agctccgggt agccgctgaa catgcgtggg ttatcaagga
    37201 acgccggtag tttctggtac atgcgcatat cgcgcagcgg gccactctca agatgctgac
    37261 gatattccgc cagtttttgc ttactgaaat cgtcgctttt catcgctgaa agcacggttt
    37321 ttgctgcagc ttccccggcg gcaatcgcca gatccatacc gcgaatggta aaaccgaggt
    37381 tcatacacat tccggcggca tcgccggcaa tcaatacgcc gtcaccgacc agctccggca
    37441 gcatgttgat gcctgcttcc ggcactacgt gagcggaata ttccaccagc ttgccacccg
    37501 cgatcagcgg tgcaacggcc ggatgttgtt tgaaatcttc cagcatttgc ggcaccgatt
    37561 tttttgcgtc atgcagatga tgcagaccac aaaccagccc cagcgacagg gtattttcat
    37621 tggtataaag gaagccgccg cccatcaggc catcggtggg tgatcccgca aacagacaag
    37681 ccgcgccctg attaccctgc aactgaaaac ggtcttcgat taccgacttc ggtaactcga
    37741 tcagttcctt cacgccaacc gccacatccg tcggtttaac gcgttttgcc atccccaact
    37801 tttcggcaag gatggaattc accccgtcag caaggatcac cgttttcgct tcaatcacat
    37861 cgccatcggc ttctacaccg acgactttgc catcgcgctg tacgaggtta tcgacacgaa
    37921 tcccggtaat taactgcgcg cccgcttctt cggcctgctc catcaaccag gcatcaaatt
    37981 tactgcgcaa aacggagtaa gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt
    38041 ccatagtcat cgctgacttt tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt
    38101 ctacgggggc ggagtcggcg aaaccaggaa taatgtgttc cagactgtgg gcatagagac
    38161 gcccgccggt gacgttcttg gcacctgcgg aattgccacg ctcgataact aacacttgcg
    38221 caccttcgcg ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga
    38281 tggcgtcaaa gatatcttcg gacataagag ctccctgtca gcggcagggc caaaatgcag
    38341 ccctgccaga gtggatcaac gcgctaaagc tgcggtcagc gccggaagga tcttcacggc
    38401 gtcgccaaca atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttgttgat
    38461 ggcgaaaatg gtttgcgatg cattagcacc aaccatgtgc tggatctgcc cggagatccc
    38521 caccgccagg tacagttcag gtttcagcat caggttggag ataccgacat agcgttcgtg
    38581 ctccatccat ttttcgtttt ccgccaccgg acgagaacag gccaactccg cacctatcgc
    38641 cttgcaaagc tgttctgcca gcgcaatgtt ctctttgctg ccaataccgc gaccgacgct
    38701 gaccaccaga cgggctttgt cgagatcgac gctgttgctc tggcgcgcct gggttgccgt
    38761 gcgggtaatc gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga
    38821 cgcgtctggc tgagccgcat cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt
    38881 ggcaatgcgt tcttcgccaa tcgccagacc accgtaaacc atgtgtttca ctgtcgcttt
    38941 accgtcctgt acgctgacgg tgctggcatc gttagacacc gccgctttaa ggcgataacc
    39001 cagttttgcc gccagtaatt tgccgcgacg ggtgtttggc agcagcacca ggccgtctgc
    39061 gccgtgctgg cgaatagtgt cagccatgac accggcgtaa tcttcgatca tccgatcgtc
    39121 cggtttgccg tttaatttcc agacatgatt agcgccgagc tggattgcct gtacgccgtc
    39181 ggcatcattg aggacaaagg tgttgatttg attagctaaa gcctgcgcac cgttcatcag
    39241 ttccggcaga cgagaagggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc
    39301 atcccctgta attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg
    39361 ccgtcgcctt caatcacgat gcgctgacgt tcgcgctgtt tcggcgcagc aacctgttgt
    39421 tcagaccagg cgtccacggc gttaaaaccg atatccgccg ccgaccatac ctggactggc
    39481 tttttcgccg cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg
    39541 gaaacagcaa caaccgcagg cagcggaatg cttaaggttt cggtttcatc ttccagttcg
    39601 cgctcaacgg tgagggtatc tgccgtcagg gaaataattt tgctgacgcc gttaactgcc
    39661 ggaatattga ggatttcgcc caccagcaga ccaacctgct gggcataaag gtcggaagaa
    39721 ccatcgccac agaggatcaa atcaaagcct gctttctggg cggctgcagc cagtgcgctc
    39781 gccgtttgtt gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcattc
    39841 gggccgcgcg atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca
    39901 cttaaggccg tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata
    39961 gcgttcagat cgtattggct tattttggca tcggctttgc tgaagtctaa tgaaccatca
    40021 gcattattga ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc
    40081 attgcatctc cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt aattacagat
    40141 atcacgccct ttaatttgga aataaaactt taatcaccaa tattgaaaat gtcacacgca
    40201 gataaatcca actttcaata ttgttaagtt cctcaccaat attgaaaaca tggcgtagca
    40261 aaaagaaatt ttcaatattg ctttatggat caccaatatt gaaagtcaga tgctattttt
    40321 ttactcacaa cacaacataa caaactgatt aataatcaaa attaacgaga aaagcgccct
    40381 ctattcaccc gaaacaaaaa tgtgataaca atcacagaat gcagcttatt gaatacccat
    40441 tatgagttag c
//
LOCUS       DAPHDX010000030        40280 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPHDX010000030 DAPHDX010000000
VERSION     DAPHDX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.030"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            124..1488
                     /locus_tag="RH058_001509"
     CDS             124..1488
                     /locus_tag="RH058_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137113.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="RH058_001510"
     CDS             complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="RH058_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV3137114.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2275..3186
                     /gene="panE"
                     /locus_tag="RH058_001511"
     CDS             2275..3186
                     /gene="panE"
                     /locus_tag="RH058_001511"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV3137115.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3149..3739
                     /gene="yajL"
                     /locus_tag="RH058_001512"
     CDS             3149..3739
                     /gene="yajL"
                     /locus_tag="RH058_001512"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV3137116.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="RH058_001513"
     CDS             complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="RH058_001513"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV3137117.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5447..5689
                     /gene="xseB"
                     /locus_tag="RH058_001514"
     CDS             5447..5689
                     /gene="xseB"
                     /locus_tag="RH058_001514"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV3137118.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5689..6588
                     /gene="ispA"
                     /locus_tag="RH058_001515"
     CDS             5689..6588
                     /gene="ispA"
                     /locus_tag="RH058_001515"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV3137119.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6613..8475
                     /gene="dxs"
                     /locus_tag="RH058_001516"
     CDS             6613..8475
                     /gene="dxs"
                     /locus_tag="RH058_001516"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV3137120.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLGAAGMEAKIKAWLA"
     gene            8530..9504
                     /gene="yajO"
                     /locus_tag="RH058_001517"
     CDS             8530..9504
                     /gene="yajO"
                     /locus_tag="RH058_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV3137121.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEVTLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9558..10076)
                     /gene="pgpA"
                     /locus_tag="RH058_001518"
     CDS             complement(9558..10076)
                     /gene="pgpA"
                     /locus_tag="RH058_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV3137122.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10054..11031)
                     /gene="thiL"
                     /locus_tag="RH058_001519"
     CDS             complement(10054..11031)
                     /gene="thiL"
                     /locus_tag="RH058_001519"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV3137123.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11109..11528)
                     /gene="nusB"
                     /locus_tag="RH058_001520"
     CDS             complement(11109..11528)
                     /gene="nusB"
                     /locus_tag="RH058_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV3137124.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11548..12018)
                     /gene="ribE"
                     /locus_tag="RH058_001521"
     CDS             complement(11548..12018)
                     /gene="ribE"
                     /locus_tag="RH058_001521"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV3137125.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12107..13210)
                     /gene="ribD"
                     /locus_tag="RH058_001522"
     CDS             complement(12107..13210)
                     /gene="ribD"
                     /locus_tag="RH058_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV3137126.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13214..13663)
                     /gene="nrdR"
                     /locus_tag="RH058_001523"
     CDS             complement(13214..13663)
                     /gene="nrdR"
                     /locus_tag="RH058_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV3137127.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13814..14353
                     /locus_tag="RH058_001524"
     CDS             13814..14353
                     /locus_tag="RH058_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV3137128.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14652..15536
                     /gene="tsx"
                     /locus_tag="RH058_001525"
     CDS             14652..15536
                     /gene="tsx"
                     /locus_tag="RH058_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV3137129.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15713..16060)
                     /locus_tag="RH058_001526"
     CDS             complement(15713..16060)
                     /locus_tag="RH058_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV3137130.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16189..17160)
                     /gene="secF"
                     /locus_tag="RH058_001527"
     CDS             complement(16189..17160)
                     /gene="secF"
                     /locus_tag="RH058_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV3137131.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17171..19018)
                     /gene="secD"
                     /locus_tag="RH058_001528"
     CDS             complement(17171..19018)
                     /gene="secD"
                     /locus_tag="RH058_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV3137132.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19046..19378)
                     /gene="yajC"
                     /locus_tag="RH058_001529"
     CDS             complement(19046..19378)
                     /gene="yajC"
                     /locus_tag="RH058_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV3137133.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19401..20528)
                     /gene="tgt"
                     /locus_tag="RH058_001530"
     CDS             complement(19401..20528)
                     /gene="tgt"
                     /locus_tag="RH058_001530"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV3137134.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20584..21654)
                     /gene="queA"
                     /locus_tag="RH058_001531"
     CDS             complement(20584..21654)
                     /gene="queA"
                     /locus_tag="RH058_001531"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV3137135.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21747..22328
                     /gene="acpH"
                     /locus_tag="RH058_001532"
     CDS             21747..22328
                     /gene="acpH"
                     /locus_tag="RH058_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV3137136.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22333..24150)
                     /gene="malZ"
                     /locus_tag="RH058_001533"
     CDS             complement(22333..24150)
                     /gene="malZ"
                     /locus_tag="RH058_001533"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV3137137.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24306..25679)
                     /gene="proY"
                     /locus_tag="RH058_001534"
     CDS             complement(24306..25679)
                     /gene="proY"
                     /locus_tag="RH058_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV3137138.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25755..27074)
                     /gene="brnQ"
                     /locus_tag="RH058_001535"
     CDS             complement(25755..27074)
                     /gene="brnQ"
                     /locus_tag="RH058_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV3137139.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27481..28776)
                     /gene="phoR"
                     /locus_tag="RH058_001536"
     CDS             complement(27481..28776)
                     /gene="phoR"
                     /locus_tag="RH058_001536"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV3137140.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28834..29523)
                     /gene="phoB"
                     /locus_tag="RH058_001537"
     CDS             complement(28834..29523)
                     /gene="phoB"
                     /locus_tag="RH058_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV3137141.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29713..30915
                     /gene="sbcD"
                     /locus_tag="RH058_001538"
     CDS             29713..30915
                     /gene="sbcD"
                     /locus_tag="RH058_001538"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV3137142.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30912..34055
                     /gene="sbcC"
                     /locus_tag="RH058_001539"
     CDS             30912..34055
                     /gene="sbcC"
                     /locus_tag="RH058_001539"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV3137143.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34181..35365
                     /gene="araJ"
                     /locus_tag="RH058_001540"
     CDS             34181..35365
                     /gene="araJ"
                     /locus_tag="RH058_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV3137144.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(35508..36416)
                     /gene="mak"
                     /locus_tag="RH058_001541"
     CDS             complement(35508..36416)
                     /gene="mak"
                     /locus_tag="RH058_001541"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV3137145.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36541..37452
                     /gene="rdgC"
                     /locus_tag="RH058_001542"
     CDS             36541..37452
                     /gene="rdgC"
                     /locus_tag="RH058_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV3137146.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37530..37613)
                     /locus_tag="RH058_001543"
     CDS             complement(37530..37613)
                     /locus_tag="RH058_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137147.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37610..37951)
                     /locus_tag="RH058_001544"
                     /pseudo
     CDS             complement(37610..37951)
                     /locus_tag="RH058_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38099..38383)
                     /gene="ppnP"
                     /locus_tag="RH058_001545"
     CDS             complement(38099..38383)
                     /gene="ppnP"
                     /locus_tag="RH058_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV3137148.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPSHSEFHLQVAEPTSYLCRYL"
     gene            complement(38455..39132)
                     /locus_tag="RH058_001546"
     CDS             complement(38455..39132)
                     /locus_tag="RH058_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV3137149.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39390..39581)
                     /gene="yaiA"
                     /locus_tag="RH058_001547"
     CDS             complement(39390..39581)
                     /gene="yaiA"
                     /locus_tag="RH058_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV3137150.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39631..40155)
                     /gene="aroL"
                     /locus_tag="RH058_001548"
     CDS             complement(39631..40155)
                     /gene="aroL"
                     /locus_tag="RH058_001548"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV3137151.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT         9988 a        10791 c        10182 g         9319 t
ORIGIN      
        1 gaatgcaaaa aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa
       61 cactctgttt acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg
      121 gtaatgaacg attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc
      181 gtattctcgt tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc
      241 atggctctgc aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg
      301 actcaggccg tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca
      361 ttaattgtcg gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac
      421 tccatctggg gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc
      481 gttatggcgc tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt
      541 atcggcgtga gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact
      601 cacaaacttg ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt
      661 gcgttgacca tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga
      721 atggtgaaag gcagtttcag taaagtgctg gcggaaccgc ggctgctaaa actcaacttt
      781 ggcattatgt gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta
      841 gctgatgcgg ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc
      901 gcctttggct cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa
      961 gtctttgtct tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcacaa
     1021 acgcagttct ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg
     1081 gaagcccttc tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg
     1141 atgggtgtct actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc
     1201 tggattgacg gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca
     1261 gtgtggctgg cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt
     1321 gaaatcccgg cggacattgc cgcaaacgaa gcgttaaaag tgcgtttgct ggaaactgaa
     1381 ggcatcaaag aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc
     1441 aaagtgacga atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga
     1501 attgcctgct gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag
     1561 gccgaatcag gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat
     1621 cgcggaagtt tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga
     1681 cagcctgcaa atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg
     1741 cctgcacttt cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct
     1801 caatgccctg tttcagtttc gcttccacaa accaggtttt accgctatga acgatattct
     1861 ccggtacatc cagcgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat
     1921 ccagcaactg attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt
     1981 cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc
     2041 tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag
     2101 atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat
     2161 aaccgacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac
     2221 aattaaggga tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa
     2281 attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag
     2341 ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagcgt gaatctggtt
     2401 gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttcttgcc
     2461 accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttacgatgc cgtcaaaagc
     2521 ctcgcgtcca cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc
     2581 atcgaagagt tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc
     2641 cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat tggtccggca
     2701 cggcaacagg atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac
     2761 gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg
     2821 attaatccac tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa
     2881 gaaattatgc agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact
     2941 tcagcagaag atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc
     3001 tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt
     3061 tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt
     3121 gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc
     3181 tggtagtgaa gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa
     3241 agtcaccact gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt
     3301 gaagctgctg gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt
     3361 actgcctggt ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac
     3421 cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac
     3481 cgtgctggtg ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa
     3541 agacaaaatt cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa
     3601 attgctgacc agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct
     3661 gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat
     3721 ttataattat tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt
     3781 tacaaccaca aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata
     3841 gagcgcctgc agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg
     3901 gtcgagatcg ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc
     3961 ttcgactttc agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc
     4021 gttcgggccg aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc
     4081 gatttcgcgg atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc
     4141 gaatttctct tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga
     4201 gataacaccg caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc
     4261 caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga
     4321 gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc
     4381 gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat
     4441 catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac
     4501 gaccggttcg aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca
     4561 cagataatgc gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa
     4621 acagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa
     4681 accaccggaa atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc
     4741 ttcgtagcgg cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac
     4801 ttccggattg gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac
     4861 gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca
     4921 gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg
     4981 catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt
     5041 cagggcgtcg cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc
     5101 ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc
     5161 ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa
     5221 caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta
     5281 tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta
     5341 acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt
     5401 ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa
     5461 tgaggctccc gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct
     5521 ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct
     5581 ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc
     5641 tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg
     5701 cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca
     5761 ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt
     5821 aagcgtctgc gacccttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac
     5881 acgctggacg cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat
     5941 gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag
     6001 tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt
     6061 ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa
     6121 ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg
     6181 gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca
     6241 ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct
     6301 ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac
     6361 atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa
     6421 cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg
     6481 gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacaatc actcgatacc
     6541 tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt
     6601 aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc
     6661 acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc
     6721 cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg
     6781 gtcgaactga ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg
     6841 gatgtggggc atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc
     6901 accatccgtc agaaaggcgg cctgcacccg ttcccgtggc gcggcgaaag cgaatatgac
     6961 gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct
     7021 gccgagaaag aaggcaaaaa tcgccgtacc gtctgtgtaa tcggcgatgg cgcgattacc
     7081 gcaggcatgg cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg
     7141 gtcctcaacg acaacgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa caaccatctg
     7201 gcgcagctgc tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc
     7261 tctggcgttc cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg
     7321 gtagtgcctg gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt
     7381 cacgatgtgc tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag
     7441 ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc
     7501 actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc
     7561 ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac
     7621 aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca
     7681 cgtaaattcc cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt
     7741 gccgccggtc tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcttg
     7801 caacgcgcct atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc
     7861 gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac
     7921 ctctcttacc tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa
     7981 tgtcgccaga tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac
     8041 ccgcgcggta acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa
     8101 ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca
     8161 gacgcggcga aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa
     8221 ccgcttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta
     8281 gaagaaaacg ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat
     8341 cgtaaaccag tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact
     8401 caggaagaaa tgcgcgccga actcggcctc ggtgccgccg gtatggaagc caaaatcaag
     8461 gcctggctgg cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg
     8521 agtggcagca tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc
     8581 ctcggctgta tgacctttgg cgagccagat cgcggtaatc acgcatggac actgccggaa
     8641 gaaagcagcc gtcccataat taaacgtgca ctggaaggcg gcataaattt ctttgatacc
     8701 gccaatagct attccgatgg cagcagcgaa gagatcgttg gtcgcgcact gcgggatttc
     8761 gcccgtcgtg aagacgtggt cgttgcgacg aaagtgttcc atcgcgttgg tgatttaccg
     8821 gaaggattat cccgtgcaca aattttgcgc tctatcgacg acagcctgcg acgtctcggc
     8881 atggattatg tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagtg
     8941 acgctggaag ctctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca
     9001 tcaatgcacg cttcgcagtt tgctcaggca ctggaactcc aaaaacagca cggctgggcg
     9061 cagtttgtca gtatgcagga tcactacaat ctgatttatc gcgaagaaga gcgcgagatg
     9121 ctgccactgt gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg
     9181 cgactgacgc gtccgtgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa
     9241 aatctctata aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc
     9301 agtgaagaac tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg
     9361 ggcattgccg caccgattat cggtacatcg cgggaagaac agcttgatga gctgctgaac
     9421 gcggtggata tcactttgaa gccagaacag attgccgaac tggaaacgcc gtataaaccg
     9481 catgcggtcg taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat
     9541 aaggcactgg tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac
     9601 aggatacctg cggagatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca
     9661 tgtacattgc gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa
     9721 atcacaaacc cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc
     9781 cacataccaa taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttt
     9841 gcggtctgat gacaaagata gacgccgata cagatcccca gcatcaccac cagcgagtag
     9901 agctgccagg gcaaaaaggt catcagatac cagaacggaa tcgctgccag cgagcccatc
     9961 gtcccaggaa cgatcgggct taatccactt ccgaatccga cagcaagcag atgccacgga
    10021 ttactcatct tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc
    10081 tttccagtca aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc
    10141 ggtcatctgc ccgatacagg taaacggtac gcccaggtgt ccgagagcca catccagcgc
    10201 gccacggttc agttccggca cagtgaaaca caactcgtaa tcttcaccgc cagagagcgc
    10261 ccagcgcagc gcctgttcag gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa
    10321 tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat
    10381 cagaccgtca gagagatcga tagctgaatt tgccagatcg cgcagtgcct gtccctgtaa
    10441 aatacgcggc gatggacgga gatgacgttt gatcaagtag tccgcatctt tagcatcggc
    10501 aacctgcaaa cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt
    10561 cacatagatc cagtcacccg gtttcgctcc agagcgcgtt aaggctcgtc ccatcggaac
    10621 aaagccgtgg atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag
    10681 ttgcatatcg taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc
    10741 ttcgtctacg tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc
    10801 tgccagatcg cttaggttca ccgccagtgc tttatacgcc agatcagcag gatcgatatc
    10861 atggaggaag tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc
    10921 ggggatattg agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact
    10981 tcttacacgg tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc
    11041 ctcagcaggt aaaaaagaaa aggccggcga tcgcggaacg gtcttccgtg aatctaccgg
    11101 cctggatatc actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg
    11161 acgaacttat ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg
    11221 gccactttgt atggcacatc gctacgttta gacagctcgt acagcgcaat gcgcagtact
    11281 gctttttcta cctgtcccag ttcttccagc aggcgggaca ggtatggctt catcagtccg
    11341 tcgaggtatg cggtgttagt cgccaccccg gccagcagct cacggaagta caggacgtca
    11401 acgtctttta catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg
    11461 gacaactgcc aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca
    11521 ggtttcacgg atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat
    11581 ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc
    11641 gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc
    11701 ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt
    11761 gccaccacga ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag
    11821 tgcacccgcc gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc
    11881 atcttttacc tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt
    11941 gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt
    12001 agcttcaata atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga
    12061 ttttagcata atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta
    12121 aatgcaggca aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt
    12181 cggctaattt ctcaagccct ggcagcgtgc ataatccgcg ggcgtcgctg cctaatagtt
    12241 taggtgcgat atagacaatc agctcatcga ctaaacccgc ctgcagcaat gcgccagcga
    12301 gcgttggccc cgcttccacc cagatgctgt taatttgctg tttacccagt tgcatcatca
    12361 gtacaaccag atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg
    12421 gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa
    12481 tgtgatgttc cggcgtcacg cgattttggc tatcaatcac aatacgtatc ggctggcgga
    12541 gattttgttg cgggtagagc gcctgagttt gttcatccag ttcggaccaa cgcaccgtta
    12601 aggcaggatc atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgcgcgc
    12661 gcagtagttg tacatcacgc cgcgcctggg gcgaagtgat ccactggctt tcgccgctcg
    12721 ccatcgccgt gcgaccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg
    12781 tgcgcatccg cttgagaaag cctttattca attgctcggc ttcactcatc atcaggccgt
    12841 ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt
    12901 taggatcttg catcgaggca accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc
    12961 acggtggcgt acgaccatga tggctacagg gttcgagtgt cacatacgcc gttgcgcctt
    13021 tggctttttc acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgctcgtt
    13081 ggtggtaacc ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt
    13141 tggggtgggt ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact
    13201 cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt
    13261 cgaaactgcg gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt
    13321 gctccatcac cagattgcca atcatcttgc tcggcacttc gcgctcaccg gtggcgcgca
    13381 gctgcgattt aatatgattg attgccattt cgacgtcatc ggaactcacc ggacgttttt
    13441 ccagcgcccg cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt
    13501 tgctttttac aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat
    13561 tacacaccag acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt
    13621 caattacctt agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg
    13681 tggttaacag aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg
    13741 agataaagga tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag
    13801 aaagggacga cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag
    13861 ccttagcgcc tgtgtgcagc agagcgaagt gcgacagatg aaacacagcg tcagcacgct
    13921 gaaccaggag atgacgcaac tcaataaaga aacggtcaaa atcactcagc aaaacaggct
    13981 gaatgcaaaa tccagcagtg gggtttacct tctgccagga gcgaaaacac cggcaagact
    14041 ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg atgcagatgg
    14101 caccaccctg acgctacgta ttcagggcga gtcgaatgat cccttgcccg cattcagcgg
    14161 aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca
    14221 gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt
    14281 acagttaaag ggtatttctg tagaacagtt agacttcgta cgtatccacg atattcaacc
    14341 tgttatgcag taaacgtatt tcggggcgat tttgtgcgtc tcgcaacatc tttccccgtc
    14401 attttgttac tccgcttaca tcaccctgat tgatagtaaa agtttgcaac aagggtgaaa
    14461 gtcagtacaa tccctgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat
    14521 agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta
    14581 tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca
    14641 gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt
    14701 cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc
    14761 agagcgttaa cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata
    14821 cctaccttga gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg
    14881 atgcgccggt attcttcggc ggtaactccg atgctaaagg tatctggaac cacggttctc
    14941 cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta
    15001 gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta
    15061 ataaagatgg tcgccagagc acctggtaca tgggtctggg taccgacatc gatactggtc
    15121 tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag
    15181 cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg
    15241 atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt
    15301 taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtactaat aactctatcg
    15361 cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt
    15421 actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact
    15481 tcaacgttcg ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgattat
    15541 gaaaatgccg ggatttattc ccggcatttc agattgttaa caacgtgcgc tttgttcatg
    15601 ccggatgcgg cgtaaacgct ttgtccgacc cacaaaagca cgtaaattca atatattgct
    15661 gaaataacgt agtcctgata agcgtagcgc atcaggcaat tttacgtttt aatcacttct
    15721 tcttgttcat catcgctttc aggtcagcga atgggttgta cttcgcttca ccgacatctt
    15781 tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttcg gtatatttcg
    15841 aatgctcatg atcgtggcaa tagagacaaa gcaattccca gttactgcca tcttccgggt
    15901 tattggtatg gtcgtgatca atgtggtgaa cagtaagttc acgcaggttg gaataaacaa
    15961 actcgcggga acagcgaccg cagacccacg gatatatttt taatgctttt tcgcgatagc
    16021 cgctttctaa ccgcgcatag ttttttggga tgatagccat gaagagttac ctggatgaaa
    16081 tgtagggtta cgcgcagttt cccaagatgc ggggatttta gcaatctcca tacatcaaaa
    16141 aaaatcccga tcttctgacc gggattttca acatcaacgg gaacttgatt acggcagaat
    16201 tgacggctga tccgcccctt ctttttccac tttctgctgc aacatgtgtt cgcgcttcat
    16261 acccagtttc agagccaacg cagatgccac atagatggaa gatgcagtac cgatggaaac
    16321 accgataagc atggtcagcg agaagccttc cagtaccgga ccaccgaaga ggtacagcat
    16381 caggataacc atcaaggtag taccggatgt gatcaaggta cggtgcagcg tctgggtcaa
    16441 ggacacgtta aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt tttcacgaat
    16501 acggtccgat accacgatac tgtcgttaag cgagtaaccg ataaccgaca tcaacgatgc
    16561 cacaatggtc aggtcaatct cgatatggaa taacgacaaa atacccagcg taatgataac
    16621 gtcgtgcgcc agcgcaataa cgacccctgc cgccagtcgc cactcaaagc gaaaacctac
    16681 gtacacaagg atagacagca gcgccgccat caacgccatc gcaccggttt gcgcaaggtc
    16741 tgcccccacg ctcggaccga cgaactcaat acgcttcact gctgcattct gattggtgga
    16801 ttcgttaatc accttcagaa cctggctgcc cagcacctga ccgccggttt cgccttcagc
    16861 aggcggcata cggaccatga tgtcgtggct gctaccaaag ttttgcagca ttggctcttc
    16921 aaaaccggct ttttgcaatg catcacgcat tacgtcaatt tcagccggtt tttcgagcgt
    16981 aatttcgata accgtaccac cggtgaaatc cagcccccag ttaaagccgc gcacgcccat
    17041 aataacgata gcggcgatta acagcagacc agagatgccg aaagcccagt agtcccagcg
    17101 cataaagtca tagactttac ggccgtggtt tagttgttca acagtatatt cctgtgccac
    17161 atcgcactcc tcagattgac agcttcttga cgcgcttgcc gccatatagc aggtttacga
    17221 tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc cacaccgata ccggtagtaa
    17281 tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag gatgatgact ttaatcagcg
    17341 tggtgatgtt cgcatcgaag atagaactga atgcgccacg ataaccttca tcaattgcct
    17401 gttgaacagt acgtccgttg ctcaactctt ctttaatacg ttcgttgatc agtacgttcg
    17461 catcgaccgc caccgcaagg gttaagacga tacccgcgat acctggcata ctcagcgttg
    17521 cgcctggcaa cagcgacata atgccgacga ttaagatcaa gttggcaatc agagcactgg
    17581 tcgcaatcag accaaacttc ttatagaaga tgatcatgaa cagaatagaa accagcagac
    17641 cggcaaggca agcctccagc ccctgttcaa tgttctgcat acccagggtt gggccaatgg
    17701 tacgttcttc aacaatctga atcggcgcga tcaacgcacc ggcacgcagc agcagtgaca
    17761 gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta cccagacgag
    17821 actggatgtt ggcgatgtta atcacctctt cctgtttcac cagaaccgca cgaccatttg
    17881 catctttctt accgctgtct ttgtactcca caaacagggt tgccatcggt ttgccgatgt
    17941 tgtccttagt gaagttagac atgatgttac caccagcgct atcgagcgag atgttaacct
    18001 gcggttggtt gtattcgtcc tggctggaag tggagtcggt gatatggtca ccggtcagaa
    18061 ttacgcgttt gtacagcaca actggctgac cttcgcgggt ctgtttcact tcagagtcgc
    18121 ccggtacgcg accggatgcc gcagcggcct ggtcaacgtt ggtgtttacc agacggaatt
    18181 ccagcgttgc cgtcgcaccc agaatctctt tcgcacgcgc agtgtcctga atacctggca
    18241 gttcaacaac gatacggtca gcaccctgac gctgaaccac cggctccgcc acgccaagtt
    18301 ggtttacacg gttacgcagg atattgatgt tctgctgcac cgcatattca cgcgcttcac
    18361 tcagacgagc atcgctcatt accgcacgca gctggttgct gccctggcta ctaatcacca
    18421 ggtccggatg gcgcttgctc agatacgcaa tggcttcatc acgagcttta gcatcgcgga
    18481 aagtaatgct caggccgtag ttgttttctt tacgaacggt ggtatacggg atgccctttt
    18541 cacgcagatc gctgcgcagg ctatcgatat tttgttcctg gagtttgcca agcgcggtgt
    18601 ccatatccac ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg agcttcatcg
    18661 gctcagcgtg aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta agcgccacga
    18721 cgtatttgtc acccataacg cccattaatg cttcacgagc gcgcagctgg gtgtcagtgg
    18781 agtcgaagcg cgcaagaata gcgccctctt ccagtgccac agacttagca gttatttttt
    18841 cttcttgtaa cgttttctgg acctggatca gcgtttgctc actggcggcg actccgcgcg
    18901 caccagtgat ctgaacagcc ggatcctcac caaacaggtt gggaagcgca tacagcagac
    18961 cgatgacaat caccacgatc agcatgacgt acttccacaa aggataacgg tttaacacgg
    19021 caattccctt agggaaaaat tttaattaca gcgccttcat ggtgcctttc ggcaggacgg
    19081 cagctacgaa gtcacgttta ataactactt cagtggtgtc attcagcgcg atagcaatgt
    19141 agccgttttc cgctactttg gttacgcgac caaccaggcc accgttcgtc agaacttcat
    19201 cacctttggc aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc tgctgtggac
    19261 gcaggatcat gaaatagaaa atcagaccga ataccaccag catcaaaatc aaagacatcg
    19321 ggctaccttg cgccggtgca cccgttgccg ctaccgcatc agaaataaaa aagctcatta
    19381 aatttccctc attattaata ttaatcaacg ttcaaaggtg gtacttctcg cccctgacgc
    19441 tggtaaaaat cagttacgaa gctctctaat ttaccctctt caatagcctt gcgtaaaccc
    19501 gccatcaaac gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc gcctaatatt
    19561 tcgttgcaac ggtcaagatg atgcaagtaa gcgcgtgaat aattgcgaca ggtgtagcaa
    19621 tcacactcag gatcgagtgg gccagtatcg ctcttatact tcgcattgcg gattttcacc
    19681 acgccatcgg tcacgaacaa atgaccattt cgggcgttgc gggttggcat tacgcagtca
    19741 aacatatcga taccacgacg tacgccttca accaggtctt ctggtttacc aacgcccatc
    19801 aggtaacgcg gtttgtctgc cggaatttgc gggcatacat gctccagaat gcggtgcata
    19861 tctgctttcg gctcacccac agccagaccg ccgacagcgt agccatcaaa accgatatct
    19921 accagacctt taacagaaat atcacgtaaa tcttcgtaaa cgctgccctg aatgatacca
    19981 aacagcgcat ttttgtttcc gagactgtca aaacgctcac ggctacgctt cgcccaacgc
    20041 agagacatct ccatggagcg ttttgcgtaa tcccagtcag caggatacgg cgtacactca
    20101 tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa tctccattga tttttcagga
    20161 tcgaggaaaa tcggatcgcc gttgatcggg ttacggaagt gcacgccctg ttcggtgatt
    20221 ttacgaatat cgccaaggct gaagacctgg aaaccgccgg agtcggtgag gatcggcccc
    20281 ttccactgca taaaatcgtg cagatcgccg tgcagtttca tgatttcctg gcccgggcgc
    20341 agccacaggt ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc aacttcttcc
    20401 ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca taaaacaagg cgtttccact
    20461 acgccacgat caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt gtccagttca
    20521 aatttcattt tttctccact acgtcagaaa aacagtccaa cgttttaaac cagcgccgcg
    20581 gaattactcc ccgacgcgct cattaattgc ctgcggattg tacgtgataa acatcgcatc
    20641 accgtaacta aaaaagcgat atttctcttc taccgctgct ttataggcgt tcatggtgtg
    20701 ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc gactctggca agtggaagtt
    20761 cgtcaccagc gcatcgacca ctttgtactg gaagcccgga tagataaaga tttgtgtatc
    20821 gtcgaagaac ggttcaatga gatcgttttt cgctgcctga gccgcgcttt ccagcgaacg
    20881 tactgaagtg gtgccaaccg caatcacccg gttaccgcgc gctttcgccg ccagtaccgc
    20941 gtctaccaca tcctgcggta cttcagcgta ttccgagtgc atgatgtgat cttcaatggt
    21001 gtcgacgcgc accggctgga aggtgcccgc accaacgtgc aacgtcacaa acgccatctc
    21061 cacgcctttg gcgcgcaatt tttccagcaa aggctcgtca aaatgcagac cagcggtcgg
    21121 ggctgcaacc gcgcccggtt tttcgctata aacggtttga taaagttcgc ggtcagcgtc
    21181 ttcgtccgga cggtcgatat acggcggcag cggcatatgg ccgatactgt tgaggatatc
    21241 cagtaccgaa cgatcatcat tgaattcgac ttcaaacagt gcgccgtggc gcgcggtcat
    21301 tgttgcgtta atactttcgt catcgcccag cagcagttct gcgccaggtt ttggcgcttt
    21361 cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc atccgctcaa ccagcacttc
    21421 aatcttgccg ccgctggctt tacgcccaaa caggcgcgcc gggatcacgc gggtattatt
    21481 aaaaaccaga agatcgccgg ggttgagctt atcaagtaaa tcggtgaaag taccgtgcgt
    21541 cagcgcgccc gtcggcccgt ccagcgacag taaacgacag ctactgcgtt caggcatggg
    21601 atagtgggca atcagggatt cgggcaattc aaaggagaaa tcggtaacgc gcatgacact
    21661 gactcagact aaaataagag gcgggtagtc tagtgccggg gcgctctccc tgcaaccttt
    21721 accccctttt ggatagaata taatcgatga attttttagc tcacctgcat ttagcccatc
    21781 tcgcggaaag ctcgctttcc ggcaatttac tggctgattt cgtacgcgga aatcctgaag
    21841 aaagttttcc gcccgacgtc gtggctggca ttcatatgca tcgacgtatc gacgtattaa
    21901 ccgacaatct gccggaagtc cgcgaagcac gggaatggtt tcgtagtgaa actcgccgcg
    21961 ttgcgcctat tacgctggat gtcatgtggg atcactttct ttcccgccac tggtcgcagc
    22021 tgtcgccgga ctttccgcta caggaatttg tctgttatgc ccgcgagcaa gtgatgacga
    22081 ttttgccgga ctcaccgcca cgttttatca atctgaacaa ttacttgtgg tcagagcagt
    22141 ggctggtgcg ctatcgcgat atggatttca tccagaacgt gttaaacggc atggcaagcc
    22201 gccgcccacg tctggatgcc ctgcgtgact cctggtacga tttagacgct cattatgacg
    22261 ccctcgaaac ccgcttctgg cagttttatc cgcggatgat ggcgcaggcg tcacacaagg
    22321 cgttataaaa cgttagttca tccataccgt agccgaaatg gcaggcaaag ccagaatccc
    22381 gtcagtcagt tgcccatgcc cttctttgca ttgccattgc acggcattga gcaacggtga
    22441 cgcgggtagc acgacttcac aagcttcgcc acggttgatt gccaccagta cacgctgctg
    22501 attcagcacg cggacaaata ccaccacgtt atcttccgca tacagcacct gacagccgcc
    22561 atgacgtagc gcctgacttt tcttacgcag cgcaatcatt cgctggtaca gcgcgaataa
    22621 cgccgtatcc tgcttttcca cctgccaagg gaacggttta cggcaaaacg gatcgttttt
    22681 gccatccagt cctacttcat caccgtaata aatgcacggt acaccaggcc aggtgaacag
    22741 ccagaccacc gccagcggca ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc
    22801 agtatcgtgg ctgtcgagct gattaaacat acgtaattgt tgttgatgag aaagccctgc
    22861 gcggtaatta tccatccagg ccatacaggt ttgggcatca atttgctgcg gatcgtaaga
    22921 gatatcggta ttggcaagaa atccccacaa cgggaatgtg aagccacgat agttcatggc
    22981 ggcatcttcc acatcggcct gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat
    23041 atacgcttcc ggctgggttt ctttcgccgc ttcggtgatc ccagcaacgt gctgcatatt
    23101 attgcgcgcc ccacccgcct cccccagcat atgcaccaca tccagccgcc agccgtccat
    23161 actccacggc gctttcagcc aatggcggac aatactgtct tccccgcgat aaatttcatt
    23221 caccagactt tccgactgat aatccagctt cggcaagctg gcatagccaa gccagtcgag
    23281 cgccgtgcca tcatcactaa acgagtacca gtcgcgccag ggcgattcag ggttgtgaca
    23341 agctcccccc gtgccacgat tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt
    23401 aaacacgcca tccagcacca gccgcattcc cagctgctgc gtattgtggc gtaagcgcag
    23461 caacgcccca tcaccgccaa actgcggatc gacatggcga taatcctcgg tatcgtattt
    23521 atgtacgctg ggagctttaa acaccggatt gagatacagc gccgtcacgc caagcttttt
    23581 cagatacggc agtttttcgc ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc
    23641 cgcctgcgcc gtgaccggtt catcccagtc acgcaagatg atctcttgtc cggctgcatg
    23701 atggtaatag acatgatcct gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg
    23761 gaagatctga taaaaaatct gatccgcagc ccattgtggg ccgatatccg gtacatcgac
    23821 ggcaaactgc tccagtcgtg ccggcggcat tcggctgaag ccctgcggtg taaaccaacg
    23881 ctggcgatcg tgccacagca gtttgaaact gtaacgccgc cggggttgtc cgctggagag
    23941 atcaatcgcc gcacgccatg cggtgacgcc aggctgctgc tgactgcgct gcttatgcat
    24001 cgatactgac atttcttcgt tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc
    24061 ttcgcccgtc agccacagtg taatgagcag ttgatctttg ctttgtttaa caaatggggg
    24121 caccggcagg tgccatgcat ttaacatcat aattcccctg tgatgaaaat ctgatcacct
    24181 tgccatagcc taatgcagga tcactcatca tctgaaggat atttggggga ggaggacggg
    24241 ggcggataac tttcggaata gccggatcag gatttcctcc tgaaccggct tacagaacgc
    24301 aagtattatt ggttttcagc cagctgacga tcgtggcggc gtttaaacat ccagccaatc
    24361 aacagcacaa caatccacgc gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa
    24421 ccaatcaacc cgataataaa gagcaggaaa atcagcccgc cgatggtcgt tgctacccca
    24481 cccggcactt taaatttcag cgccttaact tcttctggcg gcaaacggcg acggaaggca
    24541 atttgcgaca gcaggatcat aatccacacc cacaccgtgg cgaaggttgc cagcgaagcg
    24601 atcaccagga agacgttttc cggcatgatg tagttcagat acaccgcaaa cagcagcgcg
    24661 gtagtcatca ccagcaccgt aacccacgga ataccgcgac gcgacgtttt gctgaaaatt
    24721 ttcggcgcgc tgccctgctc tgccataccg tggagcatac ggcctacgcc aaatacgtca
    24781 ctgttaattg ccgacagcga agcagtcagc acaacaaagt taagaatgct ggcggcaaag
    24841 gtaatgccca tatgctggaa cgtcagcacg aacgggctac cggcagtgcc aacctgattc
    24901 cacgggtaga tagacataat gacgaacagc gtaccgacgt agaacaccag aatacgcatc
    24961 ggcacggagt taatcgcacg cggtatcgat ttctcaggat ctttcgcttc accggcggta
    25021 atcccgataa tttcgatccc accgtaagca aacatcacca tttgcaacga catcaccatc
    25081 ccaagccagc cgttactgaa gaagccgccg ttgctccaca ggttatgaat accggtcggt
    25141 tgcccgccgt tgccaatccc ccagatgatg atgccgaaac cggcgacaat catgatgatg
    25201 atggtggcga ctttaaagaa cgagaaccag aattccagct caccgaagac cttcacgctc
    25261 atcaggttta cggcgcaaat gatcagcacc acgctcagta cccaaatcca gtgcggcacc
    25321 gtcgggaacc agacacccat atagatacca aaagcggtca catcggcgat ggcgacaata
    25381 aggatttcaa agcaataggt ccagccggta atgtaacctg ccagcgggcc gaggttttcc
    25441 tgcgcataac gcgagaaaga gctggctgcc gggttatgta ccgacatttc ccccagcgca
    25501 cgcataatga tatacgccgc gataccaccg ataatatagg ccaacaacac gctcggaccg
    25561 gccattttga tggcgtctgc cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt
    25621 gccataaagc gtatgtggcg ggtacttagc ccacgcttta gcttgttctt actttccatc
    25681 aattcctaac ccatcaacac aataaaaaac cacggggcat cgcagccccg tggttaaaac
    25741 aaatgttcag tgatttagtg agcgctggag gtcacctgac gacctgccgc gcgatcccag
    25801 ataatggcca gaaccaccat caccactgtt ggcattaacc acgccagacc ttgttcggcc
    25861 agcggtaaac gctgcgccca ggacggtaag atatcgctga atgcagatgc tttgatcccg
    25921 tcgagaatac caaaaagcag gctgataaac atcggcggag caatcacgcg ggacgaatta
    25981 tgccaccatg agcgtgtaaa acttaataca accagtgcga tacacggcgg ataaatagcg
    26041 gtcagcaccg gtacggagat ctggatcagc tggcttaagc cgaggttaga aaccaccatc
    26101 gagaagccgc cgaggataaa caccagcgta cgataagaga gcggtacgta ctgggcaaag
    26161 aattctgcac aagcacaggt caggccaact gccgttacca ggcaggcgat gaagattaac
    26221 gccgccagca ggaagctacc gccgccgcca aaggtgtgct gaacgtaagc atgcagaata
    26281 gcagcgccgt ttgcagactg atcgaccagc gacgcgctgt ctgaacccag acggaacagc
    26341 gccaggtaca gcagagtcag accaacaccc gccatcaggc cagcccagac ggtataacgg
    26401 gtcagcagac gcgcttcggt aacgccacga gaacgcgccg cgttaacaat aacgatacca
    26461 aacaccattg cgcccagcgt atccatggtc agatagccgt taacgaagcc gttagaaaac
    26521 gcagcgtttt gataagcctc agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg
    26581 gcaacagaca ggatgaccag cgcgataatt ttcagcggcg caaggaagtt gcccacggta
    26641 tccagcagct tgcccggata gagcgaaacc agaataacga tagcgaaata gaccaggctg
    26701 tagataaaca gcggcagcgc ggaatcacca gtcagcggcg caatccccac ttcaaaggaa
    26761 acggtagctg tacgcggcgt agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc
    26821 agcagtacgc cagcgacttt accgattggc gtgctgaggc tgtcaacacc gccgccaact
    26881 tttgccagcg ccactaccgt tagtaccggc agaccaacgg cagtaatgag gaagccgaat
    26941 gccgcagtcc agacgtgttc gcctgcctgc aagccgacca ttggagggaa aataatgtta
    27001 cctgcgccga cgaacaacgc aaatgtcata aagcccagag cgatgatatc gcgcgatctt
    27061 aattgatggg tcataaatca tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt
    27121 gccaatccct ggggatgata cagcggtata tttaaacaat tttagcggga aatactccca
    27181 accaggcgcg gcgaagaata gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc
    27241 acctgaagcg caatttaaac gcttataacg ttttaaagca aggagggatc taaaaaccag
    27301 aggtatatag cgataagaaa tcaccaacca gtataaaaca ctatttccgt gaaaaagaca
    27361 tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga
    27421 ctgcaaagaa aataagccag catcggctgg cttatggcaa taaaagatga caaaggcgga
    27481 ttaatcgctg tttttggcaa ttaaacgttc cgggataaca aaactgaaac gtgttccttt
    27541 tcctactgta ctctcaatat tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat
    27601 cgctaaccct aatccgctac cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa
    27661 acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc ggtccgttat cttcaacgct
    27721 aaattcggca ccgtgcggca ctcgctgcca gcgtacggtg atatgcgtgc cttctggagt
    27781 atgattcacg gcgttataga ccaggttcga aatcgcactg cgtagctgat cttcgttgcc
    27841 agacactttg aggccgttat ctatctcaaa ggtaaatgtc tgttttttct gactcagagt
    27901 ctgagcctca cgctcaacaa cgcgcagcat catcggcaca tcaacctttt cattgagcaa
    27961 atgcgtcggt gcggcttcta ttttcgacag cgtcagcaac tgcttcacca gcccttccat
    28021 ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg
    28081 ctgctcatcc atcatctcca ggtaaccctg taacacggtc aatggcgtac gtaactcatg
    28141 gctcacgttg gcaaaaaagt tacgccgcgc cccttccagt tgatgcattt gcgtgacatc
    28201 acgcgccacc atcagcaact gtttgtgggt ataaggcatg acgcgaattt ccagatgccg
    28261 cccggtgttg agcaccagat tgagcgggcg agaaaaatca cgcgttttca gatattgcgt
    28321 aaactccggg taacgcagca ggttaaggat gttctgcccg ttatcttccg gccagcgcaa
    28381 accaagaatt tgttgcgcca gaccgttaca ccagaaaata ccgccctctt cagtggtcag
    28441 caccactgcg tcgggcagcg actccgcgcc gctacgaaag cgtttaatca gattgcccag
    28501 ttcacggcgg cgttttttat ttcgcagctg catctggtgt aagccgtata gtagcggttc
    28561 ccagctacca cgccccggtg gcggggtcat actacgatcc acccacagcc accatgaaag
    28621 gcgcaataaa ttccagaaat gccagataag cagtcccgtt accgatgcca gcaaaaacca
    28681 gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg aagcagcaaa gtagcagctc
    28741 cagcaccagc cttttccacg acagccgttc cagcacgtaa gatactccag ttaagaaatc
    28801 ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc
    28861 tgtaccgcgc acggtctgca ccatgcggtc atgcccgcct ggctccagtg ctttacgtag
    28921 gcgacgaatg tggacatcga ccgtacgatc ttccacatac acgttagttc cccagacgtg
    28981 gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag
    29041 tttaaattct gtcggcccca tctccagcgg ctcttcgccc gccatcactc ggtgagatgt
    29101 cgggtcgaga cttaatccct gcatctcaat cacctcttcc accgccattg gcgaaatacg
    29161 gcgcattacc gctttgattc gcgccaccag ctccttcggc gaaaacggct tggtgatata
    29221 gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa
    29281 catcaccact ggaatatccc gggtcatcga ctcgcgcttg aggtgtttga tgaactggat
    29341 accggagccg ccaggtaaca tccagtcaag gagaattaaa tccggccagg gttcattcag
    29401 ttgattcaca gcactgtcat aatcttccgc ttcgaccggc tgaaagccat tttgttcgag
    29461 cacgaagcag accatttcgc gaattggagc ttcatcttct acgaccagaa tacgtctcgc
    29521 catgatttgc cctgttgtaa taaataggtt gcgatcatta atgcgacgtc attatgcgtc
    29581 agatttatga cagatttatg aaaagctcgt cgcacatatc ttcaggttat tgatttccgt
    29641 ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa tcgcgcccat gctttttcgc
    29701 cagggaatcg ttatgcgcat ccttcacacc tcagactggc atctcggcca gaacttctac
    29761 agtaaaagcc gcgaagctga acatcaggct tttcttgact ggctgctgga aacagcacaa
    29821 acccatcagg tggatgcgat tattgttgct ggtgatgttt tcgataccgg ctcgccgccc
    29881 agttacgccc gcacgttata caaccgtttt gttgtcaatt tacagcaaac tggctgtcat
    29941 ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc
    30001 atggcgttcc tcaatacgac cgtggtcgcc agcgccggac atgcgccgca aatcttgcct
    30061 cgtcgcgacg ggacgccagg cgcagtgctg tgccccattc cgtttttacg tccgcgtgac
    30121 attattacca gccaggcggg gcttaacggt attgaaaaac agcagcattt actggcagcg
    30181 attaccgatt attaccaaca acactatgcc gatgcctgca aactgcgcgg cgatcagcct
    30241 ctgcccatca tcgccacggg acacttaacg accgtgggtg ccagtaaaag tgacgccgtg
    30301 cgtgacattt atattggcac gctggacgcg tttccagcac aaaactttcc accagccgac
    30361 tacatcgcgc tcgggcatat tcaccgcgca cagattattg gcggcatgga acatgttcgc
    30421 tattgcggct ctcccattcc actgagtttt gatgaatgcg gaaagagtaa atatgtccat
    30481 ctggtgacat tttcaaacgg caaattagag agcgtggaaa acctgaacgt accggtaacg
    30541 caacccatgg cggtgctgaa aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg
    30601 cgcgatgtat cgcaggagcc acctgtctgg ctggatatcg aaatcactac tgatgagtat
    30661 ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat cattgcctgt cgaagtattg
    30721 ctggtacgtc ggagtcgtga acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc
    30781 ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc tggcactgga agaactggat
    30841 gaatcgcagc agcaacgtct gcagcatctt ttcaccacga cgttgcatac cctcgccgga
    30901 gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg
    30961 aatggaagat tgatttcacc cgcgagccgt tcgccagcaa cgggctgttt gctattaccg
    31021 gcccaacagg tgcggggaaa accaccctgc tggacgccat ttgtctggcg ctgtatcacg
    31081 aaactccgcg tctctctaac gtttcacaat cgcaaaatga tctcatgacc cgcgataccg
    31141 ccgaatgtct ggcggaggtg gagtttgaag tgaaaggtga agcgtaccgt gcattctgga
    31201 gccagaatcg ggcgcgtaac caacccgacg gtaatttgca ggtgccacgc gtagagctgg
    31261 cgcgctgcgc cgacggcaaa attctcgccg acaaagtgaa agataagctg gaactgacag
    31321 cgacgttaac cgggctggat tacgggcgct tcacccgttc gatgctgctt tcgcaggggc
    31381 aatttgctgc cttcctgaat gccaaaccca aagaacgcgc ggaattgctc gaggagttaa
    31441 ccggcactga aatctacggg caaatctcgg cgatggtttt tgagcagcac aaatcggccc
    31501 gcacagagct ggagaagctg caagcgcagg ccagcggcgt cgcgttgctc acgccggaac
    31561 aagtgcaatc gctgacagcg agtttgcagg tacttactga cgaagaaaaa cagttactta
    31621 ccgcgcagca gcaagaacaa caatcactaa actggttaac gcgtctggac gaattgcagc
    31681 aagaagccag ccgccgtcag caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc
    31741 aacctcaact ggcggcgctt agtctggcac aaccggcacg aaatcttcgt ccacactggg
    31801 aacgcatcgc agaacacagc gcggcgctgg cgcatattcg ccagcagatt gaagaagtaa
    31861 atactcgctt acagagcaca atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc
    31921 agtcagcaga attacagcag cagcaacaaa gcctgaatac ctggttacag gaacacgacc
    31981 gcttccgtca gtggaacaac gaactggcgg gttggcgtgc gcagttctcc caacaaacca
    32041 gcgatcgcga gcatctgcgg caatggcagc aacagttaac ccatgctgag caaaaactta
    32101 atgcgcttgc ggcgatcacg ttgacgttaa ccgccgatga agttgctacc gccctggcgc
    32161 aacatgctga gcaacgccca ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc
    32221 cccaacaaaa acgtctggcg cagttaatgg tcactatcca gaatgtcact ctggaacaga
    32281 cgcaacgtaa tgccgcactt aacgaaatgc gccagcgtta taaagaaaag acgcagcaac
    32341 ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat taaaacgctg gaagcccagc
    32401 gcgcgcagtt acaggcgggt cagccttgcc cactttgtgg ttccaccagc cacccggcgg
    32461 tcgaggcgta tcaggcgctg gagcctggtg ttaatcagtc tcgattactg gcgctggaaa
    32521 acgaagttaa aaagctcggt gaagaaggtg cgacgctacg tgggcaactg gacgccttaa
    32581 caaagcagct tcagcgtgat gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac
    32641 ttactcaaca atggcaagcc gtcacggcca gcctcaatat caccttgcag ccacaggacg
    32701 atattcaacc gtggctagat gcacaagatg agcacgaacg ccagctgcgg ttactcagcc
    32761 aacgacatga attacaaggg cagattgccg cgcataatca gcaaattatc cagtatcaac
    32821 agcaaattga acaacgccag cagcagctct taacggcatt ggcgggttat gcactgacat
    32881 tgccacagga agatgaagaa gagagctggt tggcgacacg tcagcaagaa gcgcagagct
    32941 ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg tattcagcag ctgacgccga
    33001 ttctggaaac gttgccgcaa agtgatgagc tcccgcacag cgaagaaact gtggcgctgg
    33061 aaaactggcg gcaggttcat gaacaatgtc tcgcattaca cagccagcag cagacgttac
    33121 agcaacagga tgttctggcg gcgcaaagtc tgcaaaaagc ccaggcgcag tttgacaccg
    33181 cgctacaggc cagcgtcttt gacgatcagc aggcgttcct tgcggcgcta atggatgaac
    33241 aaacactaac gcagctggaa cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc
    33301 aaacgctggt cactcagaca gcagaaacgc tggcacagca tcaacaacac cgacctgacg
    33361 ggttggctct cactgtgacg gtggagcaga ttcagcaaga gttagcgcaa actcaccaaa
    33421 agttgcgtga aaacaccacg agtcaaggcg agattcgcca gcagctgaag caggatgcag
    33481 ataaccgtca gcaacaacaa accttaatgc agcaaattgc tcaaatgacg cagcaggttg
    33541 aggactgggg atatctgaat tcgctaatag gttccaaaga gggcgataaa ttccgcaagt
    33601 ttgcccaggg gctgacgctg gataatttag tccatctcgc taatcagcaa cttacccggc
    33661 tgcacgggcg ctatctgtta cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg
    33721 atacctggca ggcagatgcg gtacgcgata cccgtaccct ttccggcggc gaaagtttcc
    33781 tcgttagtct ggcgctggcg ctggcgcttt cggatctggt cagccataaa acacgtattg
    33841 actcgctgtt ccttgatgaa ggttttggca cgctggatag cgaaacgctg gataccgccc
    33901 ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat cggtgtgatt agccacgtag
    33961 aagcgatgaa agagcgtatt ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct
    34021 acagcaaact ggaaagtacg tttgcagtga aataactatt cagcaggata atgaatacag
    34081 aggggcgaat tatctcttgg ccttgctggt cgttatcctg caagctatca ctttattggc
    34141 tacggtgatt ggtagccgtt ctggtggttg tgatggtggt atgaaaaaag tcattttatc
    34201 tttggctctg ggcacgtttg gtttggggat ggccgaattt ggcattatgg gcgtgctcac
    34261 ggagctggcg cataacgtag gaatttcgat tcctgctgcc gggcatatga tctcgtatta
    34321 tgcactgggg gtggtggtcg gtgcgccaat catcgcactc ttttccagcc gctactcact
    34381 caaacatatc ttgttgtttc tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct
    34441 ctcttcgtct tacctgatgc tcgccattgg tcggctggta tccggctttc cgcatggcgc
    34501 attttttggc gtcggagcga tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc
    34561 cgccgtggcg gggatggttt ccgggatgac agtcgccaat ttgctgggca ttccgctggg
    34621 aacgtattta agtcaggaat ttagctggcg ttacaccttt ttattgatcg ctgtttttaa
    34681 tattgcggtg atggcatcgg tctatttttg ggtgccggat attcgcgacg aggcgaaagg
    34741 aaagctgcgc gaacaatttc actttttgcg cagcccggcc ccgtggttaa ttttcgccgc
    34801 caccatgttt ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc catacatgat
    34861 gtttatttcc ggtttttcgg aaacggcgat gacctttatt atgatgttag ttgggctagg
    34921 gatggtgctg ggaaatatgc taagtggcag gatttcagga cgttattcac cactgcgcat
    34981 tgcagcagtg actgacttta taattgtact ggcactgctg atgctctttt tctgcggcgg
    35041 catgaaaaca acgtcgctta tttttgcttt tatttgttgc gcgggattat ttgccctttc
    35101 agcaccgcta caaatattgt tactacaaaa cgccaaaggc ggagagttat taggtgccgc
    35161 aggtgggcaa atagcgttta acctcggtag cgccgtcggc gcatattgcg gtggtatgat
    35221 gctgacgctg gggctggcat ataattacgt ggcgctgcct gccgccctgc tttcgtttgc
    35281 tgcgatgtcg tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg cggatactcc
    35341 ggtgctggcg aaaccactgg ggtaggttat agtctcggtg ctcattactt attgccggat
    35401 gcggcgtgaa cgccttatcc gccctacgcg gttctggcac attttgcagg cctgataaga
    35461 cgcggcaagc gtcgcatcag gcatgatgcg ccaattgcct acgtttttta ctcttgtggc
    35521 cataaccacg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt acgcaccggc
    35581 gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt ttgatataaa
    35641 cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag aatattcacg
    35701 acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag ttccgctacc
    35761 ggatcgcttt cttcaaccag gctgataatt tcactgcctt tcagcgcatg tccgctcaaa
    35821 cgacgataat ctgtggcaaa tcccgtgccc gaaataaagg tttcaataca accttgttta
    35881 ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc
    35941 ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgcccg cccattgaat
    36001 gccacgcccg cgccgcatcc cgtgccgata atcacggcaa atactgtctg cgctcccgct
    36061 gccgcgccat ctactgcttc tgaaaccgcc agacagttgg cgtcatttgc cagccgcact
    36121 tcccgctgca acctcgcgct taagtcttta tcgaatggct gaccgttgag ccaggttgaa
    36181 ttggcattct taaccacacc ggtgtaaggc gaaattgagc caggaatgcc catacctacc
    36241 gttccgcgct gccctgtcgc ctgctccgcc atatcaacca acgtggcgat cgtttcaata
    36301 gtctgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg ctcccctgca
    36361 tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat acgcacggta
    36421 ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt ggcaatgcaa
    36481 ggccgccgac aattcgttat catgcccgct aaatttaacg acaaggccgt ggaaattatc
    36541 atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc gctgcgtgca
    36601 gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag ccaggacatg
    36661 gcgaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac gcacgttgcc
    36721 aatggtcaaa ttgttatctg cgcacgcaaa gaagaaaaaa tcctcccgtc tccggtgatt
    36781 aaacaggcgc tggaagcgaa aatcgccaag ctggaagcgg aacaggcgcg taagctgaag
    36841 aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact ctctgctgcc gcgagctttc
    36901 agccgtttta gccagacaat gatgtggatc gacacggtta atggtttgat tatggtggac
    36961 tgcgccagcg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag cctggggtcg
    37021 ttacctgttg taccattgag catggaaaac ccgattgaac tgacgctaac cgaatgggtt
    37081 cgctccggta gcgcagcaca gggcttccag ttgcttgatg aagccgagct gaaatcgttg
    37141 ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga agagatcacc
    37201 aatcacatcg aagccggaaa agtggtgact aaactggcgc tcgactggca gcagcgcatt
    37261 cagtttgtga tgtgcgacga tggttcgctc aagcgtctga agttctgcga cgagctgcgc
    37321 gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc
    37381 ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt aggtggcgaa
    37441 gcacaacgtt aattgctgat tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc
    37501 cggcgtacga agccacacca ggcatataat tattcgctac ggcgggcaat aatttttagc
    37561 gcagcaatat tatgcgtttt atgctgtaac ttgctccatg gacgttgtgt cattgttttt
    37621 cctcaagcca gtgacgcgct tcactgcgaa ttttccgcat tgccccggtt ttcactaaat
    37681 cccgtaacag cgaaagatcg ggttgttcga caaataagag taatgatgga tcctctttta
    37741 accacgtcgt tttcacatcg gcagcgagct gtggattatt catagctagc gaccgatgcc
    37801 gagactcggt caatgtcgtt aacaacgacg ccggggtatg tgcgttgcgt aatgccgttt
    37861 ggcgcaaatg atcattgcga ctttgcagca atgcacgcca ccattcatca tctgatttat
    37921 cttttatcgt agccagttcc gcaacaatat ctggagatct tcactgatat ttatatcaac
    37981 cttgatgaat tatgaatata tcggaaattg aaaaaatcgc agcaaaggaa atattattat
    38041 gggttaaaga gaggatattt cagaaaaatg ccctcccgtt caaggggaag gcgaggaatt
    38101 acagatagcg gcacagataa gaggtgggtt cggcaacttg cagatgaaac tcactgtgac
    38161 tgggaacatt aaacaccgaa ccggcttcat acacctgcca gtcggtcgcg tcaggcagta
    38221 acacattcag cgcgccactg attaccgtca tctcttccgg ctcagcggtg ctgaaggtgt
    38281 attcgccttc aaccataaca cccacgctgg cgcgaccagt gctgctgctg gaaaagccga
    38341 ttgatttcac tttgccggaa aagtactcat tactttgaag cataaactgg ccctgtctgg
    38401 tagttgaaca tctacttcac tataggggcc agaggcgctg gctgtcacgc aaaattacac
    38461 cagtaattcc gcagccagcc gtgcaatcaa tacgttagac agcaagacag gaacatcgag
    38521 ctgtttttgc agtaaatcgc gatgacgttg gtgaaatcct aaacaatcca gcatgatgac
    38581 atcagcccct tttgccagta attctttccc ggcatcaatg attttttgtt ctgaatcatg
    38641 aatggggtta cccaatgaaa ataccggcga tttctgcaaa atttgccatt tttgcgcctg
    38701 aacggtcagc atctcctcaa ccggaacgat aacccccacc tgatgatctt caacaataga
    38761 ggaaaccagt ggaggcaata ttcgcgacgg ctcaagaaag atcgtattac gcgcagtcat
    38821 actactaatg tttgctgtac tcattaataa aatgacgtca taaccctgat tatcgagcac
    38881 ttcaaccaca ccttgcaggt ctcgctccac tttacgacgc gaaacatggg ccagctggtt
    38941 gtcatttaat aatgtgagaa tggtgtcttc gcctgcttct ggcgcgtact ccgccatcac
    39001 ttcttcacga cttaacttcc ccagcaggct atgatgggaa atattatctt cgtcaatgta
    39061 ttccgtcagg agcggcaaaa cctcctgcat aggtacaatg ccgatggtca ggatcgccaa
    39121 cgacgcactc attgttcatc caccttactt ttctttcgtc gttaattacc gggcaagtgt
    39181 gaagcaccac tgctgacact tacttgtgct acaaatgaca aaaagcgtag cagcagacgt
    39241 gcacggcata atgtaaagat tccaaatgat tccagtaatg gattgttatt gtttaatatt
    39301 ctaattatta gaaaaacatg aattatgaaa aatgtgacgc agatcccttg tagtggcact
    39361 cagaatccct tccgagcagt ctgatgcggt cactctttgt cagggtcctc atagcgtttt
    39421 ttcgcatcca tcgcttcctg agccgtcgga tgttcactga tcaacgagtc tggtttagga
    39481 tgatcggctc tgagttggta ccaggttacc gtctgtcctg gctttccttt ttcaatcgtc
    39541 actatatatg cttcacgagg ataaggcggt ttcgttggca tcgttcttcc ttatttcacg
    39601 ggatgaacgt taagtatagg cgctctaaaa tcaacaattg atcgtctgtg ccagggcgct
    39661 acgaatttca gaaatcacct ggctgggttc gtttgttgcg tcgatgataa tatgcgcaac
    39721 ttcgcgatat agcgcatcgc gttcttccag cacttcctga acttcttcgc tcagcggttt
    39781 tcccgttaag gttggccgta aatcttcttc cggtgcagct tgcagtcggt taaccaggac
    39841 tgatactggc gcacacaaat aaaccacgat cccgttattt tgcatgaagt gacgattaaa
    39901 ttccgtcaga ataatgccgc cgcctgtagc gataacggtg gatggcgcag ttaccgcttc
    39961 cagcgccgcc gtttctctgg cgcgaaatcc cgcccactct tccctttcga cgatctccgc
    40021 gaccgtcata ttgagctgtg attgcaacca ctgatcggta tcgacaaacc gacggttaag
    40081 cgaatcggca agggccattc cgaccgttgt tttaccacag ccccgaggcc cgatcagaaa
    40141 aagaggttgt gtcatcgtgg gttttcccca ataggtcgca atgcggcgaa agccggtgtc
    40201 atgagaatag cgatcatacc atcaaactag tacaatttcg attgtaaaga aaaaattcca
    40261 cttaaagtga aaatctcaat
//
LOCUS       DAPHDX010000031        38590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPHDX010000031 DAPHDX010000000
VERSION     DAPHDX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.031"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            300..1616
                     /gene="gntT"
                     /locus_tag="RH058_001549"
     CDS             300..1616
                     /gene="gntT"
                     /locus_tag="RH058_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV3137152.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(1661..3745)
                     /gene="malQ"
                     /locus_tag="RH058_001550"
     CDS             complement(1661..3745)
                     /gene="malQ"
                     /locus_tag="RH058_001550"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV3137153.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(3755..6148)
                     /gene="malP"
                     /locus_tag="RH058_001551"
     CDS             complement(3755..6148)
                     /gene="malP"
                     /locus_tag="RH058_001551"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV3137154.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            6760..9465
                     /gene="malT"
                     /locus_tag="RH058_001552"
     CDS             6760..9465
                     /gene="malT"
                     /locus_tag="RH058_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV3137155.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(9508..10524)
                     /gene="rtcA"
                     /locus_tag="RH058_001553"
     CDS             complement(9508..10524)
                     /gene="rtcA"
                     /locus_tag="RH058_001553"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV3137156.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(10528..11754)
                     /gene="rtcB"
                     /locus_tag="RH058_001554"
     CDS             complement(10528..11754)
                     /gene="rtcB"
                     /locus_tag="RH058_001554"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV3137157.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            11943..13541
                     /gene="rtcR"
                     /locus_tag="RH058_001555"
     CDS             11943..13541
                     /gene="rtcR"
                     /locus_tag="RH058_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV3137158.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(13523..14281)
                     /locus_tag="RH058_001556"
     CDS             complement(13523..14281)
                     /locus_tag="RH058_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV3137159.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(14298..15128)
                     /gene="glpG"
                     /locus_tag="RH058_001557"
     CDS             complement(14298..15128)
                     /gene="glpG"
                     /locus_tag="RH058_001557"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV3137160.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(15173..15499)
                     /gene="glpE"
                     /locus_tag="RH058_001558"
     CDS             complement(15173..15499)
                     /gene="glpE"
                     /locus_tag="RH058_001558"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV3137161.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            15689..17194
                     /gene="glpD"
                     /locus_tag="RH058_001559"
     CDS             15689..17194
                     /gene="glpD"
                     /locus_tag="RH058_001559"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV3137162.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(17248..17853)
                     /locus_tag="RH058_001560"
     CDS             complement(17248..17853)
                     /locus_tag="RH058_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137163.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(17856..18623)
                     /locus_tag="RH058_001561"
     CDS             complement(17856..18623)
                     /locus_tag="RH058_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137164.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(18626..19336)
                     /locus_tag="RH058_001562"
     CDS             complement(18626..19336)
                     /locus_tag="RH058_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137165.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(19351..20859)
                     /locus_tag="RH058_001563"
     CDS             complement(19351..20859)
                     /locus_tag="RH058_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV3137166.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(20984..23431)
                     /gene="glgP"
                     /locus_tag="RH058_001564"
     CDS             complement(20984..23431)
                     /gene="glgP"
                     /locus_tag="RH058_001564"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV3137167.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(23450..24883)
                     /gene="glgA"
                     /locus_tag="RH058_001565"
     CDS             complement(23450..24883)
                     /gene="glgA"
                     /locus_tag="RH058_001565"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV3137168.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(24883..26178)
                     /gene="glgC"
                     /locus_tag="RH058_001566"
     CDS             complement(24883..26178)
                     /gene="glgC"
                     /locus_tag="RH058_001566"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV3137169.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(26196..28169)
                     /gene="glgX"
                     /locus_tag="RH058_001567"
     CDS             complement(26196..28169)
                     /gene="glgX"
                     /locus_tag="RH058_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV3137170.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNTYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(28166..30352)
                     /gene="glgB"
                     /locus_tag="RH058_001568"
     CDS             complement(28166..30352)
                     /gene="glgB"
                     /locus_tag="RH058_001568"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV3137171.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(30625..31728)
                     /gene="asd"
                     /locus_tag="RH058_001569"
     CDS             complement(30625..31728)
                     /gene="asd"
                     /locus_tag="RH058_001569"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV3137172.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            31920..32513
                     /gene="yhgN"
                     /locus_tag="RH058_001570"
     CDS             31920..32513
                     /gene="yhgN"
                     /locus_tag="RH058_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV3137173.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(32570..33910)
                     /gene="gntU"
                     /locus_tag="RH058_001571"
     CDS             complement(32570..33910)
                     /gene="gntU"
                     /locus_tag="RH058_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV3137174.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(33914..34441)
                     /gene="gntK"
                     /locus_tag="RH058_001572"
     CDS             complement(33914..34441)
                     /gene="gntK"
                     /locus_tag="RH058_001572"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV3137175.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(34580..35575)
                     /gene="gntR"
                     /locus_tag="RH058_001573"
     CDS             complement(34580..35575)
                     /gene="gntR"
                     /locus_tag="RH058_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV3137176.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(35799..36494)
                     /gene="yhhW"
                     /locus_tag="RH058_001574"
     CDS             complement(35799..36494)
                     /gene="yhhW"
                     /locus_tag="RH058_001574"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV3137177.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(36617..37654)
                     /locus_tag="RH058_001575"
     CDS             complement(36617..37654)
                     /locus_tag="RH058_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3137178.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            37986..38474
                     /gene="yhhY"
                     /locus_tag="RH058_001576"
     CDS             37986..38474
                     /gene="yhhY"
                     /locus_tag="RH058_001576"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3137179.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         9395 a        10461 c         9582 g         9152 t
ORIGIN      
        1 gaacatgtgc cagacgttgg cgcagcattg ctttggcaaa tccctccctt cgtttctccg
       61 cgataatatg accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc
      121 cagatgttac ccgtatcatt cacatgggta ccaaacatac tcctgacatc tgactacaat
      181 aattagtttt agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa
      241 ttagagtttg tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca
      301 tgccattagt cattgttgct atcggtgtaa tcttgttgtt gctcctgatg atccgcttca
      361 aaatgaacgg cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa
      421 tgccgctgga taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc
      481 ttgccctgat catgggtttt ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg
      541 cacaacgtat cgccaccacg ctgattgcca aatttggtaa aaagcacatc cagtgggcgg
      601 tggtgttaac cggttttacc gttggttttg ccctgttcta tgaagtgggc ttcgtgctga
      661 tgctgcctct ggtgtttacc atcgcggctt ccgcgaatat tccactgctg tatgttggtg
      721 taccaatggc ggctgcactg tctgtgaccc acggcttcct gccaccgcat ccgggcccga
      781 ctgcgattgc caccattttc aatgccgata tgggtaaaac cctgctgtac ggtactattc
      841 tggcaatccc gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg aaaggtatcg
      901 ataagccaat cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa gagatgccga
      961 gctttggcgt cagcgtctgg acttctctgg tgccggttgt gctgatggcg atgcgtgcga
     1021 ttgccgaaat gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg
     1081 acccggtaat ggcaacgctg attgccgtgc tgattgcgat gttcaccttt ggtctgaacc
     1141 gtggtcgttc aatggatcag attaacgaca cgctggtttc ttccatcaaa atcattgcga
     1201 tgatgctgtt gatcatcggt ggtggcggtg cgttcaagca ggtgctggta gacagcggcg
     1261 tggacaaata cattgcttcc atgatgcacg aaaccaacat ttctccgctg ctgatggcct
     1321 ggtcgattgc tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg
     1381 cgggtggtat cgcggcaccg ctgattgcaa cgacgggtgt tagcccagag ctgatggtta
     1441 ttgcggtggg ttccggtagt gtgattttct ctcatgtgaa cgatccgggc ttctggctgt
     1501 tcaaagagta ctttaacctg actatcggcg agaccatcaa atcctggtcg atgctggaaa
     1561 ccattatctc ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg atttgataac
     1621 actgactgcc ggatgcatcg catccggcat caaccgcact ctacttcttc ttcgctgcag
     1681 ctctgcgccg tctgtccaaa tccttcagca acttgttcac gccatcatcg gcaaacatcg
     1741 tctcaagcgt tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg gtgccaggaa
     1801 tattcaccgg ttcggccata tccagccagt cttccggctg tagtcctaac agagcactgt
     1861 tactgtcggc aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc gacatcaacg
     1921 atgccttatg cccggcacgt tttggcagac aaccatattt atgcagtgca tccagcagcc
     1981 cttgcttcgc cagttcgcga tcctgataca gaccgcgcag taccacttca tccggataca
     2041 gccccagggt tttgcccagc gttagatccc cgctctccca gtaaccgcgt agcgtaggca
     2101 ggtcatgtgt cgccgcaacc gccatcgact gctccggata cgctttcggt gcacggaacg
     2161 tcttctcgtg gtcgttttcg aaatagagca ctttgtagga gtacacaccg ctgctgcgca
     2221 gcttaccgac aatctctacc ggtacggtac cgagatcttc accaatcacc atacagcgat
     2281 gacgtttact ttcgagtgcc agaatcgaga gcagatcatc caccggatag tgaacatacg
     2341 cgccctgatc tgccgtctcg ccatacggta tccaccacaa acgcagcatc gacatcacat
     2401 ggtcaattcg taatgcgccg cagttttgca tattggcacg caacagctcg ataaacggtt
     2461 cataggcacg cgcggtgatg atatgcgggt ccattggcgg taatccccag ttctgcccca
     2521 acgggccgag gatatccggc ggcgcgccaa ccgacgcttt caggcaatat agttcacggt
     2581 cacaccaggt ttccgcccca ccttccgcta cgccaaccgc cagatcacga tacaagccaa
     2641 tcggcatttc atagccctgg cttatctccc agcaggcggc aaactggctg taagccagcc
     2701 actgcaacca gagataaaaa tcgacgtcat tacgatgttc ttcgcagaac tgacgcactt
     2761 ctggtgaatc cacgttctga tacatctctg gccatgcagg ccagccccag cgcatttcgt
     2821 cctctttcac ttgctgggca tgtagcgcat caaaggctgc ctgccagaac aggctgtcgc
     2881 cctgctctgc aacaaactgg cgaaacgcgg tcatctgctc atcatcacgt tgcgcgaaac
     2941 ctttccacgc cattcgtaat gctgtcattt ttagggcggt aaccgtggag taatcgaccc
     3001 agtcggcatc gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc caccaggcct
     3061 gagcctcttc gctaagatgg aaatcttcaa cggcgttaac gtcgatataa atcacattca
     3121 gccaacggcg agaagacggg ctgtatgggc tggcactctc cggatttgcc ggatagagcg
     3181 catgaatcgg gttcaggccg atgaacgacc cgccacgttt tgccacatcc accagcatcg
     3241 ctttgagatc gccaaaatcc ccaatacccc agtttttttc cgatcgcagc gtataaagct
     3301 gaacgcaggc accccacagc ttttgtttat tcagcaacgc ctgcggttcg taacagcgtt
     3361 tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt gtcagcgtgt
     3421 gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtt acatggcctt
     3481 tgtactgtgt tccttcttcg gtggtcagca gccagctata ttcgccgctg ccctccacca
     3541 ccatcggcat ttttttgccg ctggtataaa ccatgacatt cgggactggc gttaccgcca
     3601 ctttcgtggc ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa
     3661 tcgactgcgg tttaccgtgg gcattgatgt aattggggct aatccccgcc gccagcgcgg
     3721 cattatccag acgtttgctt tccatcgagc ttccttagcg ttttgcctgc cagatacgag
     3781 cctgataatc gcgaatagag cgatccgagc taaacatacc gcagcgggcg gtattgagga
     3841 tcgccgcgcg agtccaggcc tcctggtcgc ggtacagcac atccacctgc ttttgtgcct
     3901 caacataggc tgcgaaatcc gccatcacca gatacggatc gccgccttgt ttgccgatac
     3961 tgtgcagcat ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg ctttccagct
     4021 ctttcaatac tgcgtccagc accttatctt tcttccgcca tttcaccggg tcgtagcctt
     4081 tggccagaat agccttcact tgttccacgg tatgaccaaa aataaagata ttttcttcac
     4141 cgactttctc ggcgatttca acgttcgcac catccagcgt accgacagta agcgcaccat
     4201 tgagcgccag tttcatattg ccggtaccgg aagcttcttt acctgcagtc gaaatttgtt
     4261 cggagatatc cgccgccggg atcagttttt ccgccgccga aacgcaataa tccggcagga
     4321 acaccacctt caacttatcg ccaaccagtg gatcgttgtt gatcacgtca gccactttgt
     4381 tgatcgcaaa gataatattc ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga
     4441 ggaagacgcg cggtacgcga tcagcctgcg ggttttcacg aatttctttg tacaacgcca
     4501 gaatatgcag cagattcagg tgctggcgtt tgtactcatg cagacgtttg atctgaatat
     4561 cgaaaatcgc ctgtggattg atctcaatac cggtacgaac tttcacaaac tccgccagac
     4621 ggactttatt cgcctgcttg atctcgcgat attgctgacg gaatttcgca tcatcagcga
     4681 atttttccag attgatcagc tgatcgagat cgttagccca ctctttttgc agtgatttat
     4741 ccaacagagc cgccagtgca gggttgcact gtttgatcca gcgacgtggg gtaataccgt
     4801 tggtgacgtt atggaatttg ttcggccata gctggtgata ttccgggaac agatctttca
     4861 ccaccagatc cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca accacacaca
     4921 ggttcgccat atgcacttgt ttatcgtgca ccaccgccag tttggcccac actttttcat
     4981 cgcccggcca ggttttctcc accagcgttt taaagcgagt attaatttcg ttaataatct
     5041 gcatatggcg cggcagtaag cctttcacca gtttcacatc ccagcgttcc agcgcttctg
     5101 gcatcagggt atggttggtg taagcgaaag ttttgctggt aatggcccag gcgtcatccc
     5161 agctcatctg gtgctcatcg atcagcacgc gcagcagttc tggaatcgcg atagttgggt
     5221 gggtatcgtt cagctgaata acttcgtaat ccgccagttc gtgcagttta cgccccgcca
     5281 gatgatggcg acgcaaaata tccgctaccg aacaggcaca ctggaagtac tgctgcatca
     5341 agcgcagctt tttaccggca gtatggttgt cgtttggata gagaactttg gtcagttttt
     5401 ccgcattgat gccctgctgt tcggcacgca ggaaatcacc gtcgttaaat ttagtcagat
     5461 caaacggatg cgcgtgcgtc gcctgccaca gacgcagcgg ctgcgccacg ccattacgat
     5521 agccgacaac ggggagatcc cacgcttgac cggtaatggt aaactccggc tcccagcgtc
     5581 cgtctttcgt cactttaccg ccaatcccta cctgcacatc cagtgcttcg ttgtggcgga
     5641 accacgggta gttactgcga tgccagtcat ccggcgcttc aacctgtttg ccatcgacaa
     5701 aagactggcg gaacaaacca tattgatagt tcagaccgta acccgtcgca gactgaccga
     5761 cagttgccat tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg ttacccagcg
     5821 ccgggtcgat ctcttcttcc agcaggtccg tcagattgat gtcataagcc ttcaacgaat
     5881 cctgtacatc ctgataccag ccgagattca acaggttgtt gcccgtcagg cgaccaatca
     5941 aaaactccat tgagatgtag ttaacatgtc gctgattcgc caccggcttg gcgaatggct
     6001 gagcacgcag catttcggcc agtgcttcac tcactgctag ccaccactgg cgaggagtca
     6061 tttcagccgc agaatttaag ccataacgct gccactgacg tgaaagcgct tcctgaaatt
     6121 gcttatcgtt aaaaataggt tgtgacatag gagttccact tttcttagat tttcaacaca
     6181 acgttatcgc tagtttgcca ggctcgatgt tgaccttcct catcctgcgg gggattaggc
     6241 agggaggagt tgcggggatg agcaaggaaa tgtgatctca accacttaaa gctagtgcaa
     6301 accacaggat tagcagcaaa tcaatgcaat acagcgcaga aaatctgtat ctaagtgcaa
     6361 aaaatggccg ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta agtaaaggag
     6421 tgaaacagtt tcataagtaa aatatccagt gtgctccatc tcattcttaa tagatttatt
     6481 aagatcatct ttttagatgg cactttcatc aggaatgaag aagaaaccct tgcttaaatg
     6541 aatctgatga acataaggga aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt
     6601 tgttaataaa gatttggaat tgtgacacag tgcaaattca gacacataaa aaaacgtcat
     6661 cgcttgcatt agaaaggttt ctggccgacc ttataaccat taattacgaa gcgcaaaaaa
     6721 aataatattt cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaacta
     6781 agtcgtccgg ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc
     6841 ggcgcgaaca acttccggct ggcgctgatc acgagtcctg cgggctacgg aaagaccacc
     6901 ctcatttccc agtgggcggc aggcaaaaac gatatcggct ggtactcgct ggatgaaggt
     6961 gataaccagc aagagcgttt cgccagctat ctcattgccg ccgtgcagca ggcaaccaac
     7021 ggtcactgtg cgatatgtga gacgatggcg caaaaacggc aatatgccag cctgacgtca
     7081 ctcttcgccc agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc
     7141 gatgactatc atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc
     7201 catcaaccag aaaatctcac cctggtggtg ttgtcacgca accttccgca actgggcatt
     7261 gccaatctgc gtgttcgtga tcaactgctg gaaattggca gtcagcaact ggcatttacc
     7321 catcaggaag cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agctgcagaa
     7381 agcagtcgga tttgcgatga cgtttccggt tgggcgacgg cactacagct aatcgccctc
     7441 tccgcccggc agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat
     7501 gccagccatc tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg
     7561 cgccattttc tgttgaaaag cgccattttg cgctcaatga acgatgccct catcacccgt
     7621 gtgaccggcg aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca ggggctgttt
     7681 ttacagcgga tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggcaacttc
     7741 ctgcgccagc gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc
     7801 gcagaaagct ggatggccca gggatttccc agcgaagcga ttcatcatgc gctggcggca
     7861 ggcgatgcgc tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat
     7921 agcgaactgt cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa
     7981 aatccgcagt tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg ctatggcgaa
     8041 gttaacaccc tgctagcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg
     8101 cacgcagaat ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tccggatgaa
     8161 gcggaacggc tggcaaaact ggcactggaa gagctgccgc cgggctggtt ctatagccgc
     8221 attgtggcaa cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca
     8281 ctggcgctaa tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc
     8341 ttatggagtt taatccagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg
     8401 tgggaaacac aggaaaaagc gttccagctg atcaacgagc agcatctgga acagctgccg
     8461 atgcatgagt ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat
     8521 gaagccgaag cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca gccacagcaa
     8581 cagcttcaat gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac
     8641 gcccgtagcc agctgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac
     8701 tggatctcta acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc
     8761 gccgctgcca actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg
     8821 caaggtcaat ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg
     8881 gaaattgttc tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc
     8941 aaccgtaacc tgttgctgct taatcaactg tactggcagg ccggacgtaa aagtgacgcc
     9001 cagcgcgtgt tgctggacgc attaaaactg gcgaatcgca ccggatttat cagccatttt
     9061 gtcatcgaag gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact caatacgctg
     9121 ccggaactgg aacagcatcg cgcacagcgt attctgcgag aaatcaatca acatcatcgg
     9181 cataaattcg cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg
     9241 ccagaactga tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatt
     9301 tactccggtt acagcaatga acaaattgcg ggcgaactgg aagtggcggc aaccaccatc
     9361 aagacgcata tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg
     9421 cagcacgcgc agaaactgct gaagatgatg ggttatgggg tgtgagttta gccggataat
     9481 gcattggatc cggtttacat ctttacatca ttcaatactc acccgcgtta cgccatctgt
     9541 ttctatcaaa ccaaaccgca ccggcaagaa acgctccacc accgcgatat tggtcagcag
     9601 atggcatgaa ggatgggcga ccttaaattc tcccgcgccc gccagcgcca tcggcagtac
     9661 cagttggtcg gcgagatatt cccccaccgc cgctgggctt gccaggtagc gtttcacctc
     9721 tttcactaac tgcgcggcta ccacctcggc actgacgcgc ttttcaccga cgacgaaaaa
     9781 gcgttcagtg atattttcac tttcgacttc cagcgagacg gtattacccg gcccctggtc
     9841 gcgcggcagg ttatgaatat tctgttcatg cagggaaaaa ctccccgcca gtgtagcgat
     9901 ttcacgctca gcaacatggc gcggcacacc agctaataga acttctccac gcatctgcac
     9961 aatgttcccg cgctcgccaa gttgcaaggt gttaaacgat gccacgggcg agacttccgt
    10021 tgccaccacg ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg
    10081 aattcctatt ttcgccagca gcggctccag cacccggcgg ataaaatccg caggcggggc
    10141 cgacgggtta tcggtgcctc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag
    10201 cgcgggcagc accgtttgca gcaccagcgt acagcttccg gcactaccga tagcaaagcg
    10261 gtactcgccg ccgcgcacgg tgccaggccg gaagaccaga cgctgcgacc ccagctccgc
    10321 gccttccacc gttgccctac aaatttccgc agccgctttt accgcggtca gatgctggcg
    10381 caacagcccc ggtttcgctc gcccggcacg aatgctggtg atggtaaatg gctggccggt
    10441 tatcatcgac aggctcagcg ccgagcgcag gatctgcccg ccaccttcgc cctgtgcgcc
    10501 atccagcgca atcatccttt tcatcattta tccttttacg cacaccacct gacgcagggt
    10561 atagataact tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc
    10621 catcgggatt tcgtcgatca cttcggcatc tttacggcat tccacatgcg cggtggcacg
    10681 aatttgatct tccacgctga acagtttttt cgctttagta cggctcatta cccgcccggc
    10741 accgtggctg cacgaacaga acgactcttc atttcccagc ccacggacga taaagctttt
    10801 cgctcccatc gaaccgggaa taattccata ttgaccagca cgcgcagaca ccgcgccttt
    10861 acgcgtcacg tagatctctt cgccaaagtg ctgttctttt tgcacatagt tgtggtgaca
    10921 gttgatctct tccatcgcca gcgtttgctg ttgtcttacc gttttctgcg taacgctctg
    10981 caacgctgtt actacgtttt ccatcattgc atcgcggtta aggctggcaa aaagctgcgc
    11041 ccaggccacc gctttcaggt aatcatcaaa gtattccgta ccttccataa agtacgccag
    11101 atcacgcgac ggcaacgttt caagctggtc ctgcatctct ttttgcgcca gatcgataaa
    11161 gtaagtcccg atggcgttac caattccgcg tgaaccggag tgcagcataa tccacacctg
    11221 atccgactca tcaagacaga tttcaataaa atggttaccc gttcccagcg ttcccagatg
    11281 tttatagtta ttggtattca ggaaacgcgg atatttttgc gttaaccact gataaccggc
    11341 ttccagctca gcccatttag catcgacgtt aacaggtgga ttttcccagg cacctttatc
    11401 acgtttacaa cggccagtgg tacgcccgtg cggcacggcc gtttcaatcg cctgacgcag
    11461 ctctgccagg ttttcaggca ggtcttccgc cgttaacgcg gtacgcagcg cgttcattcc
    11521 acagccaata tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct
    11581 accaatggtg gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat
    11641 aaacggcatc ttcgccgtat taataagttg ctgacgcgca tcggcctcta ccggcacgcc
    11701 tttggtccac atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt
    11761 tttctctttt cgttgtttgc tgtcctgata attgcaaccg tcgtgccaga aaattaaata
    11821 agcagcttaa ttttttaatt cattgtttta aaaaagatta tatctttacg tccgtaaccg
    11881 gagatttccc gcaaagccaa tttaccgata atgaaatatc gtcttttata aggatatcta
    11941 agatgcgtaa aacagtggct tttggctttg tcggtaccgt actggattat gccgggcgcg
    12001 gcagtcagcg ctggtcaaaa tggcgtccga cactctgttt atgccagcaa gaatcgttgg
    12061 tcatcgatcg actggaattg ttgcacgacg cccgctcgcg ctcgctattt gaaacgctta
    12121 aacgcgatat cgccagcgtt tcgccagaaa cagaagtggt gagcgttgag attgaactgc
    12181 ataacccgtg ggatttcgaa gaggtctacg cctgcctgca tgatttcgcc cgtggttacg
    12241 agtttcagcc agaaaaagaa gactatttaa ttcacatcac caccggtacc cacgtcgcgc
    12301 agatttgctg gtttctgctg gcagaagcgc gttacctgcc cgcccggctg atacaatctt
    12361 cacctccgcg caaaaaagaa cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg
    12421 atttaagccg ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg
    12481 attttcttaa gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga
    12541 tcgaaaaagt ggcgatcaaa tcccgcgcgc cgattctgct taacggtcca accggcgcgg
    12601 gcaagtcatt tctggcgcga cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg
    12661 gcgcgtttgt ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt
    12721 ttggtcatgt aaaaggcgcg tttaccgggg cgcgggaatc tcgtgaaggt ttattacgca
    12781 gcgccaacgg cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacaag
    12841 caatgctgct aaaagccatt gaagagaaaa ccttttaccc gtttggcagc gatcgccagg
    12901 tgagcagtga ttttcagctt atcgccggaa cggtgcgcga tttgcgccag ctggttgccg
    12961 aaggcaaatt tcgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg
    13021 gtctacgcca gcgccaggaa gatattgaac cgaaccttga ttatgaagtg gagcgccacg
    13081 ccacactcac tggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt
    13141 ttgcgacctc tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca
    13201 cgcggatggc cacctttgcc actagcggac gcatcactct ggacgtggtt gaagatgaga
    13261 taaaccgtct gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg
    13321 gcgcagaggc agaaaacatc gacctttttg acaggctgca actggaacac gtgatagctc
    13381 tctgtcgcca ggcaaagtcg ctttccgctg ccggacgcct gctttttgat gtttcgcgcc
    13441 agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc
    13501 tgacgtggga agccgtgcag gatcagcaca gctccagttg aatatgatgg tccgtcagca
    13561 cctgcatcac gcttactggc ggcggggcgt cggtgtagac ggcatctacc atgctgatgc
    13621 tgcccatatt gaccatcgcg ttacggccaa atttcgagtg atcgacaacc agcataacgt
    13681 ggcgcgagtt ctcaataatg gcgcgtttgg tgcgaacttc gtggtaatcg aactccagca
    13741 gcgagccgtc gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg
    13801 agataaaatc gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac
    13861 cggcgagaat aatgcggaaa tcttctttta ccatcaacgt gttagcaaca ttgagattgt
    13921 tggtgacaat acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg
    13981 tgccgatatc gataaacagt gtcgagccat tggggatttg ctccgccact ttgcgggcaa
    14041 tcctctcttt ttcttcggtc tgggtggctt tacgatcgtg ccacggcgtg ttaaccgaac
    14101 tggaaggcag cgccgcaccg ccatggtgac gcaggatcag gttttgctcc gccagctcat
    14161 tgaggtcgcg gcgaatagtc tgcgggctga cggagaaatg ctctaccagc tcttcagtac
    14221 tgacataacc ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca
    14281 tttataaatc cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc
    14341 gctaaaccca cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa
    14401 tcaaaccatc cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa
    14461 atgccacttt gcggatcgcg ttcgccacgt agccagacgt agcccatcag cgcatacacc
    14521 acgccagaaa gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac
    14581 agggcgctaa tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgca
    14641 ccgccgagat accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt
    14701 aacgcgtggg tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc
    14761 agccataaca tcacttcctg atcgccgaga atttgcatgg cgataaacac caccacgcag
    14821 gcgatcatca ccacccaggt taccggacct gcgcgttcac gcaaggcagc aaagaaagga
    14881 taacggcgat aatgcagacc actgccggta tggcccgcct gccagctggc cgccagataa
    14941 cgcggatctg ccgggttttc gagaaaacgc gccagctccg cccgcacgcg ctcggcctgg
    15001 gactcatccg ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc
    15061 tgcgtcgcca tataatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc
    15121 atcaacatcg ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc
    15181 cgtacgccac ctctgcggga aactgacgct gccaggcttc aaaaccgccg tcaatgctat
    15241 agaccacatc gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattgccgt
    15301 gataacacat caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg
    15361 tgtcgttggt taaatggaaa gcctgcaccg catgccccat agcgaaactc tgtggatcgc
    15421 gaatatcgac cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa
    15481 tacattcgaa ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc
    15541 acagtttacg tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt
    15601 tttttcaatg ttacctaaag cgcgattctt tgctaatatg ttcgataacg aacatttatg
    15661 agctttaacg aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg
    15721 cggcatcaat ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat
    15781 gctggaggcg caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg
    15841 tggcctgcgc taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg
    15901 tgaagtgctg ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca
    15961 tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt acgatcatct
    16021 gggtaaacgc accagcctgc cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt
    16081 aaaaccggaa attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct
    16141 ggtactcgcc aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg
    16201 cgccacctct gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac
    16261 cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa
    16321 acagttcttc gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg
    16381 cagccatatt gtggtgccgc gtgtgcatac ccagaagcag gcctacattc tgcaaaacga
    16441 agataaacgt attgtgttcg tgatcccgtg gatggacgag ttttccatca tcggcaccac
    16501 cgatgtggaa tacaaaggtg atccgaaagc agtgaagatt gaagagagtg aaatcaatta
    16561 cctgctgaaa gtgtataaca cgcactttaa aaagcagtta agccgtgacg atatcgtctg
    16621 gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aggctattac
    16681 ccgtgattac acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt
    16741 cggcggtaag ttgaccacct accgcaaact ggcggaacat gcgctggaaa aactgacgcc
    16801 gtattatcag ggcattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat
    16861 tgaaggcgac cgcgacgatt atgccgctcg cctgcgccgc cgctatccgt tcctgactga
    16921 atcgctggcg cgtcattacg ctcgcactta cggcagcaac agcgagctgc tgctcggcaa
    16981 tgcgggaacg gtaagcgatc tcggggaaga tttcggtcat gagttctacg aagcggagct
    17041 gaaatacctg gtggatcacg aatgggtccg ccgcgccgac gacgccctgt ggcgtcgcac
    17101 aaaacaaggc atgtggctaa atgcggatca acaatctcgt gtgagtcagt ggctggtgga
    17161 gtatacgcag cagaagttgt cgctggcgtc gtaaggatga tgcccggttg tgaagtgcaa
    17221 ccgggctatt tggattcctt agactattca ttgtaaataa ttgaataacg gcccctgagc
    17281 taaaatcgcc aaacttaagc acgttaccca atcgccccac ttcttcctct ggtttctatt
    17341 ttttctgcac caatggcaat aatatcccaa tgcaaaacca agctgtgcac taatcgggcc
    17401 gacaaaatca gtccaaggct gcggccaaaa aggggaaaaa ttcaggggaa gccagacaaa
    17461 cgtcagaaag aaatttcgga tattttccag caacgctgat ttctctggag aaatataaag
    17521 aatgaaattc gagagaatta accagaaata gaccggcgaa aagaccagcc agcaatgcag
    17581 tctgtttttt aattcaggag gcgttctgcg ggagaattgc caaccgatgc cagccattac
    17641 actgaaaaaa agtgcatatt caaccattaa ctcaagttgt agttcctgaa gagaaatacg
    17701 cacaaccagg agcggaaagg agaatattat ttctgccaga aaagttaacg tgaatataag
    17761 tacggctaag acaataaccg tcggccatac caataatggc acgataacaa aaccgagaat
    17821 tacaggggga atataaagcc ggaatttaat catttttatt ttcctggtga ttcggatgat
    17881 gcgtcataca tcgcgcataa acactcatca attatccagt cgtctaccca gacctgatgc
    17941 tgtgtgccct gttctggctg ctttggtggg ttctcaatcg cctgccgcgc gcgttctttc
    18001 aagatggatt gattgttgaa ggcccaggca ttgatttggg tggtaatgac gttatcgata
    18061 tcgtcctggt tatctctgat ataatcctca caatatctgt tcaataaaca ggcaagagaa
    18121 acggtcgacc aaaagccggg aactgaatta atggcatcca gcaatggcgc aagagcgata
    18181 tcggcacgga aaaacatttc cagcatatac tgtaattcga cagcttcatc gccggatacc
    18241 caatcagtca tatactggca ggcaaatcgt tgcaaaattt tccattcatc ctcacgccaa
    18301 ttttctttac tggcaaggtt tatacgccgt agagaaaata cttcgtgaat attgtcaaac
    18361 tgcccctgtg caacaaattc cagtaaacgc ggcagaaaat agcggacctc atcgctattt
    18421 tgcggatcag gccctgggct actattccat gcattgatta aggaatacga gatcgctcgt
    18481 aacgatgtac tccgtaatac cttttgttcc tgctgactca ggcaatattc acaacaaacc
    18541 acaaatttct gcggcaccgg ataacgacga aaaatatgat aaatctgctc aattaatgcc
    18601 tgcatggcgg ggctgggttg cataattacc ccctcgcccg ctgatcgcgt gtaaacggtt
    18661 gataaacaaa acgagcattc gccacgttca ggcgaataat ggcactacga gagagggaaa
    18721 agctggggcg aatgcaacta ttgcagtctc gacagctttc ttcagcgcaa ttttccactg
    18781 tgtaataacg agcgtagagt ttcagttccg cttgcttatc acgaaatccg cgtaaactgt
    18841 tatcgttatg aaatttccag gcatctttaa agacctgaaa aatgtagata tcctcaatcc
    18901 aggactcaca ttttttaagc gggcgctttg ctatgatcac tcgctcatcc gggtttagca
    18961 tcgccgtaaa ttgaacggtg aggtcatttg tcgtgttcag atcacattga acatctggta
    19021 catcactgat ataggggtca aatccaaagt cagcaccaca ggcacaaaga cggctaatgg
    19081 tggcggtatc gagggaaaat cccggccata cattgctgga cgttaagcca atatttaatg
    19141 tgacattata tcgaagcaat ccctcaagac cgtcagcacc gacgaacttt tccccttcat
    19201 ccagggcctg gttaatgagt tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt
    19261 tggcaatcgt aatatccacg cctgtgaatg gatcagtacc gacaaggctt gcaaataatg
    19321 tattaactaa ttccattttt attatccgcc ttaatgcaac gttttataca tcggcacata
    19381 acctacatct tccaccaggc tctgcccctg cggcgttaaa aaccactcca ccaatttttg
    19441 tgtttctgac gtcatatttt cccgcgttac cataaaggca tcgacgatat agggatattt
    19501 gccgttgcga atgttttcgg cagtcggtgc aataccgtta atcgctagca gttttatatt
    19561 tttatcggcg ttcatttgtg ttgcgtaata acggaaggtg taacctattg cgttgtttgt
    19621 attacggtat tcggcaacga ctttaatcat tccctccatc atacttgcca cttctgtttc
    19681 ctgcggcgag atcatgcgga catttttcat gacctgggat tgcatcaccg tctggctacc
    19741 agagtcttcc gggcgttgcc aggtctggat ctcctgatcg ttaccgccaa cagtacgcca
    19801 gttggtaatt gcaccactga agatgtcacg cacctgttgt tcagtcaggg aattaaccgg
    19861 gttatctaca ttgacgatga aaacaaacgc ttcacgggca aaaggcgtgt aaatcaaagt
    19921 gacgcccgat tcctccgcgc gttttttctg cccaccggaa ggttgagcca caaagataat
    19981 atcggcatta cctttaacaa tcttgttata tgcttcggga gtacgagagt tcgccagata
    20041 ttcccattcg tgaaaatctt ccggcaaaac gcttagtgca taaaaggcag aggcataaat
    20101 ggggtacgcc gccgttgcgc catcaagtcg cggccagttt tgcgtgaact gaatttgcgg
    20161 tttaccacgc agtggtgtca gttgattatt gagtttatcg ggtcgccagg cccaggtatc
    20221 aatattttca cgcacaccga cgccgggatt aagctgttta tataaataag cctggtaacc
    20281 ggcgacaaaa ccaagcatga gaaggataaa caagagagaa ttacggcaac gtaacccgga
    20341 cgtgcccgtt aaaggatatc tggtcggcca gccgtatccc agcgaaaaac tgatatgcgc
    20401 gccaacaggt ataatgacta accaccagta ctcaccaccg actaatgagg ctaacaaaac
    20461 gcccagccag gggagcataa gatcagccaa ccaacccgct cccatctggc gaaattgata
    20521 atctgccaga tgcagcgcga ttatccagac ccccagggtt aataatgcgg gtactaaaaa
    20581 aagacattgt cgccagacgg ctatttctct tttttctggt gcactatcgt ccgcccacac
    20641 tcgccccgag aaaaatagca ttaatgcgcc cagcgccagc ataaaaatgt tgagcagaag
    20701 gtaaattgtt ggcgtgataa agatattaca aacttcaata ataccggcaa gtccgacacc
    20761 cagcatggca ataaccgccg ccaaaaactg cgccagaatg gggatgccga aaaaagtcat
    20821 taccagcgag gtcaaaatcc attttctgtt ttgcattatt ctttccattc tttctgaatg
    20881 gcgaaattat actccccgag tccccttgcc ccttctggac acttttccga aatgatggcg
    20941 aaaaaaacgg gaccctttgg ccccgttcta tttattagtg aaattacaat ctcaccggat
    21001 caatatgcca gatatgatcg gcgtactctt tgatagtacg gtcagaagag aagtagccca
    21061 tattggcaat gttcagcatc gcttttgcgg tccactcttc ctgaagctcg tagagttcgt
    21121 cgactttatc ctgacaatcg acatagctgc gataatccgc cagtacctgg tagtgatcgc
    21181 cgaagttgat cagcgaatca accagatcgc gatagcgacc cggatcttcc ggactgaata
    21241 caccgctgcc gatttgcgtc agcacctgat gcagctcctc atctttctcg tagtattcac
    21301 gcggtttgta gccctgacga cgcagttctt ccacttcttc cgctgtgtta ccaaagataa
    21361 agatattgtc agcaccgaca tgatccagca tctcgacatt cgcaccgtcc aacgtaccga
    21421 tagtcagcgc accgttaagc gcaaacttca tgttactggt gccggaagct tccgtccccg
    21481 ccagcgaaat ctgttcagac agatcggccg ccggaatgat caactgcgcc aggctaacgc
    21541 tgtagttcgg gatgaacacg actttcagct tgtcgccaat ctgcggatcg ttgttgatca
    21601 ctttcgctac gtcattgatc aaatgaataa tgtgcttcgc catgtaatag gccgaagccg
    21661 ccttaccgcc aaaaatattc acgcgcggta cccacttcgc atccgggtcg gccttgatgc
    21721 ggttatagcg ggtaatcaca tgcaacacat tcatcaattg acgtttgtat tcgtgaatac
    21781 gtttgatttg tacatcgaac aacgcctttg gattcaccac cacattcagc tgctgggcga
    21841 tatactctgc cagacgcttt ttgttctcca gcttcgcctg atgcacagcg tgattaacca
    21901 ttgggaaatc acagtgttgt tgcagctcat taagcaggct aaggtcggtg cgccagttac
    21961 ggcccaggtg ttcgtccagc acggctgaaa gcgatgggtt cgctaccgct agccagcgac
    22021 gcggcgtcac accgttggtg acgttggtga aacgacccgg gaagattttc gcaaagtcgg
    22081 caaacaacga ttgcaccatc agattagagt gcagttccga tacaccgtta actttgtggc
    22141 tcacaacaac cgccagccag gccatacgca cacgacgacc gttggattca tcaatgatcg
    22201 acgcccgtcc cagcagatcg gtatcgttcg gatactgttc ctgcaaggtt ttcaggaaat
    22261 agtcgttgat ttcaaagatg atctgcaggt gacgcggcag aattttaccc agcatatcaa
    22321 ccggccaggt ttccagcgcc tcgctcatca gcgtgtggtt agtgtaggag aagacctgac
    22381 aacacacctc aaacgcgtcg tcccagctaa attggtgctc atcgatcagc agacgcatca
    22441 tctcaggaat cgacagtacc ggatgggtat cattgagatg aatcgcgatt ttatccgcca
    22501 ggttatcgta ggttttatgc aactgataat ggcggcttaa aatgtcctga atggtcgagg
    22561 aaaccaggaa gtattcctga cgcaggcgca gctcacgccc ggagtaggtg gagtcatccg
    22621 gatacagtac gcgagatacg ttctcggagt ggtttttatc ttccactgcc gcgaagtagt
    22681 caccctggtt gaatttaccg aggttaattt cgctactggc ttgggcactc cacaaacgca
    22741 acgtgttggt cgcgtcggtg tcgtaaccag ggattatctg atcgtaagcg actcccagaa
    22801 tctcttcggt ttcaatccag cgcgtttttt taccttcctg ctgaatgcga ccaccaaaac
    22861 ggactttata gcgcgtgttg tggcgtttga attcccacgg gttaccgtat tccagccagt
    22921 agtctggcga ctctttctgg ctaccgttaa cgatgttctg cttgaacata ccgtagtcat
    22981 agcggatgcc gtaaccgcgc cccggcaacc ctaacgtcgc cagagaatca aggaagcaag
    23041 ccgccagacg tcccaggcca ccgttaccga ggcctgggtc attttcttca tcaatcagct
    23101 cttcgagatt taaccccatc gcttccagtg cgccctgtac atcttcgtaa attcctagcg
    23161 acaacatggc gttggagagc gtacggccaa tcaaaaactc catcgacagg tagtaaacct
    23221 gacgagtttc ttgcgacaac tgggcacggt ttgaacgtaa ccagcgctcc acgagacgat
    23281 cgcgcacagc aaataacgtt gcgttcagcc attcatgttt attggcgacg accggatcct
    23341 ttccaatcgt aaacatcagc ttgtaagcga tagagtgctt aagagcttct acgctaagcg
    23401 tgggcgatga atatgtaaac ggagcattca tataggcgtt tcctgaaaac tatttcaagc
    23461 gatagtaaag ctcacggtac gacttcgccg cgacctgcca gctaaaatcc attgccatag
    23521 cctgacgttg cacaaaccgc cacagtgaag gacgggacca cagtacaaaa gcacgtcgaa
    23581 tagcccgtaa cagcgaccag gcattactat cttcaaagac aaacccactg gcgacgccat
    23641 ctgcaaggtt ctcaagagaa cagtcagaaa ccgtatcagc aagcccaccg gtgcgccgca
    23701 ctaacggcag cgtaccgtac ttcaatccat aaagttgcgt taagccgcac ggttcaaaac
    23761 ggctgggcac cagaatgacg tccgcgccgc ccataatgcg atgcgaaaat gcttcgtgat
    23821 agccaatctg aacgcccacc tggccggggt attccgctgc cgccgcaagg aaaccttcct
    23881 gcagcaccgg atcgcccgcg ccgagtagcg ccagctgccc gccctgctcc agaagacccg
    23941 gtaaggcttc cagcaccagg tcgagacctt tctggctggt cagacggctc accactgcaa
    24001 aaagcggcac tttatcgtca accttaagcc ccattgcgat ttgtaactgg cgcttatttt
    24061 ccgctttatc ttccaacgta tcgcgggtgt aacgcgaggc caacagtaag tccgtctctg
    24121 gactccagat tttctcgtcc acgccgttca gtacgccgga aagacgccct tcacggtgac
    24181 gctgttgcaa cagaccttcc ataccgtagg caaactgcgg ttcggtgatc tcgcgagcgt
    24241 aggttggact gaccgccgta atgtgatcgg catagtacag accggccttc aggaaagaga
    24301 tttgtccgtt gaattccagc ccatgaatat taaagaatga ccatggcaat tggatgtcat
    24361 tcatatgatg tgcataaaac atgccttgat aggccaggtt gtgcacagta aacaccgact
    24421 tcgccggacg cccgcgcgcc gccagatacg caggcgcaag gcctgcatgc cagtcgtgcg
    24481 catgcaccac atcaggacgc cagaatgggt caagcccgct ggccatttct gccccaaccc
    24541 accccagcag cgcaaaacgc aatacgttgt cggtataggc aaataagttg gtatcgtgat
    24601 acgggcttcc cggacgatca tagagatgcg gcgcgtcaat caggtatatg ccaaccccgt
    24661 tgtaatgacc gaacaacagc gtgatatgtc cggcgaaggt atcacgacgg gatactacct
    24721 gcgcatcggt aacgccacgg cgaatatcgg gaaatgcagg caacagtacg cgagcgtcaa
    24781 cgccgtctgc gatttgtgct gcgggtaatg ccccaataac atcagccaga ccgccggttt
    24841 taagcagcgg gaacatctct gaacatacat gtaaaacctg cattatcgct cctgtttatg
    24901 ccctaacttc cgtagcattt cgcgcgttac cagcacgatg ccttcttctg aacgatagaa
    24961 acgacgtgca tcttcctctg cgttttcacc aatcaccatg ccttccggaa taacacaagc
    25021 acgatcgatg acgcagcggc gcagacggca cgagcgacct acccatactt ccggtaacaa
    25081 tacggcggaa tcaatgttgc agaatgaatt cacgcgaacg cgcgagaaca gaacggactg
    25141 caccaccacc gaaccggaga tcacacaacc gccggatacc agtgagttaa gggtcatccc
    25201 gtggctaccg gagcgatcct gcacgaattt cgctggcggt aatgattcat tgtaggtgcg
    25261 aattggccaa ttgcgatcgt acatatccag ttccggcacc acagaggcca gatcgaggtt
    25321 cgctttccag taagcttcca gcgtacccac atcgcgccag tacggctcgg catccgggtc
    25381 ggattgtacg caagagagcg ggaacgggtg cgcataggcc agaccggctt cggtgatctt
    25441 gggaatcaaa tctttgccaa agtcgtggct ggagttctca tcacgatcgt cttcttccag
    25501 cagttcatac agatagtcgg cgtcaaagac gtagataccc atactcgcca gagatttgct
    25561 cggatcgttc ggcattgacg gtgggttagc aggtttttca acgaattcga taattttatc
    25621 gttctcatca accgccataa cgccaaatgc ggaggcttct tcaatcggta ctggcataca
    25681 agcaacggtg caacgtgcgc ctttttcgac gtgatcgata agcatacgcg agtagtcttg
    25741 cttgtagata tggtcgcccg ccaggatcac cacgtattcc gctttataac ggcggataat
    25801 gtcgaggttt tgggtgaccg catctgcggt gccgcgatac cagttttccc ctttcattct
    25861 ctgctgtgct ggcagcagat cgacaaactc gttcatttct tcattgaaga atgaccagcc
    25921 gcgctgaatg tgctgcacca gagtgtggga ctggtactgg gtgatcacgc ccatacgacg
    25981 gatcccggag ttgatgcagt tagacagcgc aaagtcgata atgcggaact taccgccgaa
    26041 gtgtacggcc ggttttgctc gcttattggt taaatccttc aggcgggtac cacgtcctcc
    26101 cgccagtatc agggcaacag atttcaatgg cagctggcgc gccaacatta agtgatcgtt
    26161 cttctctaaa ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg
    26221 tgtgcaggtc cctgccagac agccgtaatc actgggttat cctctccagc gaatggggga
    26281 atggcgtgcc actccccagc aggtaaaaca atctctgtta cctcaagcgt ggcgttaatt
    26341 gcgatcaaaa agcgatccga gagcagaatt tgcagctgtt tcggcccgtt ttgccactca
    26401 tccgtgctta aaggttgagc atatcgattt agccaacgga cattgccgtc gccttcttcc
    26461 caccagcgat tctccaccaa agcgggaatg cgcttgcgca gatggattaa cgcggcggta
    26521 aatgcggtta aaccactgct tgcctgcgac cagtccaacc aggttaattg gttatcctga
    26581 cagtaggtat tgttattgcc atgctggctg tgaccatgtt cgtcaccggc cagtaacatc
    26641 ggcgtaccct gggagagcaa caacgttgtt aacagggcgt gaatgctgtc gcgccgccgt
    26701 tcaaccaggt caagagaacc gcctaaccct tctttaccat gattgttact gtaattgttg
    26761 ttggtcccgt cgcgattttc ttctccgttt gcttcattgt gtttatggtt gaagcaaacg
    26821 cagtcgcgaa gcgtaaaacc gtcatgcgcg gtgacgagat taatcgcggc actcggcaga
    26881 cgaccattac gtttaaaaac atcgctggag gcagcaaaac gcccggcaaa cgcccccaga
    26941 ggcaaatcat aatgtagcca gaaacgacgg gcagcatcgc ggaaatgatc gttccactcg
    27001 gcaaacagcg gcgggaaatt tcccacctga taaccaccag gagcgatatc ccacggttca
    27061 gcaattaact tcacctgcga gagcaccggg cagttctgga tagcggtaaa caacggcgca
    27121 tcctgacgga actctggcgt acggcccatg actgccgcca gatcaaagcg gaaaccatcg
    27181 acgtggcagg tttctaccca ataacgcagg caggcgctgg cataatccac caccgccgga
    27241 tgactcaaat tgagcgtgtt gccgcaaccg gtccagttgt gataatcgcc gtcttctctt
    27301 atccaataat agctacggtt atcgatccca cgcagcgaga ataacgggcc gtcgaggtcc
    27361 agttccgcac tatggttgag cacgatatca agaatgactt cgatacccgc tttatgcagt
    27421 gctttgattg catcgcgaaa ctcatccagc gccgtttctg gcgagcaggc atacgccgga
    27481 tgcagcgcaa acatcgccac cgggttgtaa ccccagtagt tacttagccc catgcgttgc
    27541 agacgtggtt cactggcaaa ctgcgccact ggcagcagtt ccagcgcggt aatgcccaat
    27601 tgtttcaaat agttgatcat caccggatgc ccgagggctt tataagtgcc acggatctcg
    27661 accgggatct ccgggtgcaa gtacgttaat cctttgacat gggcttcata aatgatggtg
    27721 ctgccccacg gcgtgcgcgg cggggcatca tcttcccagt catagtgatc aaccaccact
    27781 acgcatttcg gcgcaatggc ggcgttgtcg cgatagtcag gttcattatg accggcgtgc
    27841 agcagcgggt tatctttaaa ctccccgtca atttgccgcg cgcaaggatc aatcaacaac
    27901 ttcgccgggt taaagcgatg cccctcggcg ggttgccagg ggccatgaac gcgataacca
    27961 taacgcaaac ccgggcgcgc atccggcaga taaccgtgcc aaatgtcgcc actgtgccct
    28021 ggcaagtcat agcgatgttc ctggccattg gcgtcaaaga cacacagttc tacccgctcg
    28081 gcatgagcgg agaaaagtgt gaagttgacg ccctgaccgt cgtaatgcgc gccgagggga
    28141 gcgggtttgc caatggcgag ttgtgtcatt ctgcctcccg aaccagccag atagtggcca
    28201 gcggtggtag cgtcaggctt agtgaatgct gacgaccgtg gctggcaatc tcatcgctgt
    28261 gtaccgtgcc gccattgcct gcattactgc cgtgatagtg catggaatcg gtattgagga
    28321 tttcacgcca tttgcccggc tggtttatgc cgaagcgata atcatgacgc ggtaccggcg
    28381 taaagttact ggcaacgatg atttcgttac cctctttatc gcgacgcaca aagatcagca
    28441 ccgagcgttc tttgtcatcc accaccagcc attcaaagcc gtacgggtca aaatccagtt
    28501 catgcattgc tttatggtgg cggtaggtga ggttcagatc gcgcaccaga cgctggacac
    28561 cgtggtgcca gttatcgccg ccttccaaca gatgccagtc gaggctggcg tcatggttcc
    28621 actcgcggcc ctgggcaaat tcgttaccca tgaacagtag tttcttgccc gggaatgccc
    28681 acatccagcc atagtaggcg cgcaggttcg cgaatttctg ccatgcgtcg cccggcatgc
    28741 ggtcgagaat cgatttttta ccgtggacca cttcatcatg cgacaacggc aggacgaagt
    28801 tttcagtgta gttgtagaga atcccgaagg tcagtttatc gtgatgatac tgacgataaa
    28861 ccgggtcgag cttcatgtag tccagggtgt catgcatcca gccgaggttc cacttgtacc
    28921 agaagcccag accgcccata tcctgcggac gagaaacgcc agggaaatcg gtagactcct
    28981 cagccattgt caccgcaccg gaaacctgct caccaagaat acggttggta ttacgcaaga
    29041 attcaatcgc ttcaagattc tcgcgcccgc caaattcgtt cgggatccac tccccctctt
    29101 tacggctgta gtcgcgataa atcattgacg ccaccgcatc gacgcgcagc gcatcaatac
    29161 caaaacgttc aatccagtaa agcgcgttac cgacgaggaa gttactgact tcacggcgac
    29221 catagttgta gatcagcgtg ttccagtcct gatgatagcc ttcacgcgga tcgctgtgtt
    29281 catacaagtt cgtgccatca aattcggcaa gcgcaaagtc atcagtcggg aagtggcctg
    29341 gcacccagtc gagaatcacg ttcagaccag ctgcgtgtgc ggcatcaatg aaataacgga
    29401 agtcgtcgcg agtaccaaaa cggcgggttg gtgcatacag gccggttggc tgataacccc
    29461 aactgccatc gaagggatgc tcgttaatgg gcagtagttc gaggtgggta aagcccatcc
    29521 atttagcata aggcaccagt tgatcggcca gctcgcggta gctcaaccag aaattgttgt
    29581 cggtgtggcg acgccaggaa cccaggtgaa cttcataaat agagattggc gcatcaaact
    29641 gattcgcttt tttgcgctct tcagtctgta caaccttttc cggcagcccg caaataagag
    29701 acgcggtttc cgggcgcatt tgcgcttcga aggcataagg gtcggacttc agacgcaagt
    29761 tgccattggc atcaatcatc tcgtatttat agagctgacc gttatgcgcc ccagggataa
    29821 acagttccca gatgccgctc tctttacgca ggcgcatcgg gtgacggcga ccgtcccagt
    29881 agttgaattg cccaaccacc gagacccgac gggcgtttgg agcccagaca gagaaacgcg
    29941 tacctgtgac gccatccata gtatctgcat gcgcgcctaa ggtttcatac gggcgcaggt
    30001 gagtaccttc agataatagc caggcatcca tttcctggat tagcggacca aaacggtaag
    30061 gatcatcaat caggttttgc tgaccatgcc agacaacagc caactgatag cggaaaaaat
    30121 tcttacgtcg cggaatgacg ccgctaaaga atccccgtga gtcgagacac tccagttttg
    30181 cgagtttgcg cccggttttc ggttcaatca cccacacatc ggtagcgtcg ggtaaaaggg
    30241 cacggacttc cagtcccgcg gtggttttat gcattcccag tacggaaaaa ggatccgcaa
    30301 aatggcctgc aattagcgcg ttaatcacgt ctctatcgat acgatcggac atgcttgtct
    30361 tcctgtttta ttgtgtcacc ccatccaatt ggatttttga cttctggttg tgacattttt
    30421 ttgacctgaa cggcgcagca ctctgtgcat cctctctgcg tcgtcctcac ttcaggtaag
    30481 gctgtgaata ctcatgtatt cagccaccct taaagaatag ccaatgctct atttaactcc
    30541 cggtaaatca tgaaacatct gcgcttactc ctgtattacg cactaacagg ggcggcatcg
    30601 cgccccagat ttaatgaata aagattacgc cagttgacga agcatccgac gcagcggctc
    30661 cgcggccccc cacagcagct ggtcgcccac ggtaaaggct gacaggaact ctggtcccat
    30721 attcagctta cgcaggcggc ctaccggcgt ggtcagcgtg ccggtaacgg cagctggggt
    30781 tagctcacgc atagtgattt cccgatcgtt cggaacgact ttcgcccacg gattgtgcgc
    30841 agccagcagt tcttccacgg tcggaataga cacatctttt ttcaatttaa tagtgaatgc
    30901 ctggctgtgg cagcgcaatg ccccgacacg cacacataaa ccatctaccg gaattacgga
    30961 agatgtgttg aggatcttgt tggtttccgc ctgccctttc cactcttcgc ggctctgacc
    31021 gttatcgagc tgtttgtcga tccacggaat caggctaccc gccagcggca cgccaaagtt
    31081 atccaccggc agctcaccgc tacgggttaa ggttgtgact ttgcgttcga tatcgagaat
    31141 agcagaggac ggggtcgcga gttcatctgc cacatggcca tacagatggc ccatctgggt
    31201 taataactca cgcatatgtc gcgcaccacc gccggaagcg gcctggtagg ttgcaacgga
    31261 cacccaatca acaagatcat tggcgaataa accacccaac gacatcaaca tcaggcttac
    31321 ggtacagtta ccgccaacaa aagtcctgat gccattattt aatccgtcgg taatgacgtc
    31381 ctgattgacg gggtcaagaa tgatgatggc gtcatctttc atgcgcagag acgatgctgc
    31441 gtcaatccag taaccttgcc atccgctttc acgaagcttt ggatagattt cgttggtata
    31501 atcgccgccc tgacaggtca caatgatatc gagggccttt agcgcctcca gatcaaaggc
    31561 atcctgaagt gtgccagtgg ttccgccaaa agacggcgca gcctggccaa gctgagaagt
    31621 agaaaagaag acagggcgaa tggcgtcgaa gtcgcgctct tcaaccatgc gttgcatgag
    31681 aacggagccg accataccgc gccagccgat aaaaccaaca tttttcataa gcgttttttc
    31741 ctgcaaagat gtgtgctgta taaatgtgcc ggtctcctct tggcacatct ttcaccatac
    31801 aaaaagcagc caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg
    31861 acttatcctg ctttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta
    31921 tgaatgaaat catttctgca gcagttttat tgatcctgat tatggatccg ctcggaaacc
    31981 tacctatttt catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg
    32041 tgcgagagtt gcttattgct ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa
    32101 ttctggcatt tctcagcctg cgagcagaaa ccgtctccat ttctggcggc atcattctgt
    32161 ttctaatcgc cattaagatg attttcccca gcgcttcagg aaatagcagc gggcttccgg
    32221 caggtgaaga gccatttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc
    32281 tcgccacgct gatgttgttg tctcatcagt acccgaatca gatggggcat ctggtgattg
    32341 ctctgctgct ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac
    32401 gtctgctggg cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga
    32461 tgatggcaac ccagatgttc ctcgacggca ttcgaatgtg gatgaagggg taacgaggag
    32521 agtttttcga aaagaacccg gtagcgtcac aactaccggg cgaacaaact taactcaaca
    32581 gctggaacgc aatcatccca acgatggcac cgacagtgcc gaggatggtt tccatcatgg
    32641 tccaggtttt cagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac aaccagaaac
    32701 cggcgtcgtt aacgtggctg acaacaatcg aaccaccagc gatacaaatc gacagcgccg
    32761 ccatttgcgc accggagtag ttcagttgtt caataaccgg catcaccagt cctaccgccg
    32821 ttaaacaggc tacggtggca gaaccctgaa tgatgcgcac tgcagcggcc agcacgaagc
    32881 aggtgatggc tatcggcagg cccatgccag ttaacgcttc gcccagtgcc ggacctacgc
    32941 cagagtcaac cagcacctgt ttgaacacgc cacctgcacc aatcaccagc agaatgatcc
    33001 ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc atgccctgac
    33061 gcattgccag gccataaatc gctaccagac aagcaaccag aatcgcggta aacggatgac
    33121 caataaactc gaaccattcg taagcggtag agccttctgg cacaaaacgc gcggcaatgg
    33181 ttttcagccc caccagcacc agcggcaaca ggatcagcga caggctgaat ccgaaagatg
    33241 gcatcttgcc ttcgccgaga tgcggttcgc tgatgtcgtc aggaatatgc aactccacgt
    33301 agcggctgat gaaattaccc cacagcggcc cggcaataat cattcccgga attgccgcac
    33361 acaggccaat caggatcatc cagccaaaat cggcgttcat ctgcgatgcc agcagcattg
    33421 gcgctggccc tggcaccagg aacgccgccg ctgccgccac gcctgcgaat aatgggatta
    33481 ccagcttcac caggttcgta ccggtgtggc gcgccattga gaaagcaacg ctaatcagca
    33541 gaacaatcgc cacttcaaag aacagcggta gcgcacagac cagccccgca aggccgatgg
    33601 cataatgcgc gcggctgtga ccgaaggatt taagcatttt gacggcaatc tgatcgactg
    33661 cgccggtttc atgtaagatc ttgccaaaca tagctcccag ggcgacaacc accgccagga
    33721 agccgagggt gcctcccatc cctttttcca tcgtcgctgc gattttatcg agcggcatac
    33781 cggaaaaaag gccagccccc atggacacca ccatcaaagc caggaaagcg tgcatacgcg
    33841 ccttcatgac taaaaacagc agcagtaaaa cagaccctac tgctgttaaa acaagcgtta
    33901 atgtagtcac tacttatttg ccttttttaa taacctcaat ggtgcttgcc acaacacctt
    33961 ccagcggttg atcgatatcc accaccagta catcagtttc gtccgcaccc ggctcctgca
    34021 gcgtttcaaa ctgcgtcacc aacatttggg ttttaaagaa atggcctttg cgcgctttca
    34081 ggcggctttc aatcacgtca aaatcgcctt tcaggtagat gaaagagaga ttcggattac
    34141 cttcacgcag caagtcgcga tagtgttttt tcaatgcaga acagacgatc agtgacactt
    34201 tattagtgcg ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac ggtttgcgat
    34261 cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat attgcgccgt ggatggagga
    34321 aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac ttcactggcg accgcagatt
    34381 tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg atcatggtta gtcgtgctca
    34441 aagtggtgcc cccacaatac aagaattaaa aatgttacgg gtaactgtta tcggtaacat
    34501 tgtccagccg gacaatatca gaagcaatat ccatccgtgc cttaagtgta taagtgtgag
    34561 ctacttcaaa tttgtaggct taaatagatc cgcccggtga caaggtgaaa cctaaatcta
    34621 acattttcgg tgtcacagat tcgccacgaa tacgcgccag caggcgttca gcgccaatac
    34681 tgcccatccg ctcacgcggc gtcagcacgc tcgctaaccg cggctccatc acctggccaa
    34741 tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg aaccttcaat cccagacgct
    34801 gacattcaaa cgccgcgccg accgccaggt catcgttggt acagaacacg ccatccagct
    34861 gcggatattc ccgccgcgcc tggcgaatca gttcaatacc ggaagagtaa gaagaagatt
    34921 gctcaaccat cacgctatac ggcaccagac ctgcatccag catcgcctgt tcgtatccct
    34981 tctgtttgat gatagtacgt tcgtcgagac gcgcgccgag ataggcaatg tggcaatgcc
    35041 cgcgagcaat aatggcggtg gtcatctggc gtgccgcttc aaagttatca aaaccgacgg
    35101 cgatatcgag gcacggcgac tggctgtcca tgagttccac caccgggatc cccgccactt
    35161 caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt gaggatcagt ccgtcgatat
    35221 tccatgagag catggattcg agacgttctt gctccatttc cggtttataa ccgtagtgcg
    35281 ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc gattccgcgt aatacttccg
    35341 cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat cgcccggctg gtggcgttag
    35401 agaggatatc gggcgcacga ttgggaatat agcccagttc atcaagagcg gcggcaatct
    35461 tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa aaaacggctg accgtcattt
    35521 tggtcacgcc tacacggtca gccacatcct gaagtacggg tcttttcttt ttcatcgtcc
    35581 tgaaggtaca aaagagatag attctcttag tttaacacgg acgcatcaga accttcccgg
    35641 ggaaaacagt gcttgtttac aattattaga tatacactaa atcaattgca tcgcattgtg
    35701 ctaacgacgc ggcaaaaccc cggaagcgtg catcagcacg cgaccggagc gagcgaggaa
    35761 gcctgcaaaa aggcaggctt caaagatgca gttgagtttt aaaccggcgg cagatcgaac
    35821 agtaacactt cgctgtcgct atccgcatgg atggagattg cctgctcatc ccagattgcc
    35881 agaccatcgc tggtcgaggc tttcacgccg ttaatggtga cattgccttt caccacctgg
    35941 atccagacgc ggcgttcagc ggcaatctga tgcaccgact gctcatcttt cagcaacgcc
    36001 caacgataca gttccatatc ctgatgcact ttcagcgaac catctcgcgc atccggcgag
    36061 agcaccagct gtttgccctg cacggcatcg aagcgacgct gttcataacg cggcgtaatg
    36121 ccattttctt caggcatgat ccagatctga tacagatgca gacgctcggt gctgtttggg
    36181 ttgtactctg agtgacgaat acccgtaccc gcactcataa tctggaactc acccgccgga
    36241 acctgctctt tattgcccat gctgtcctga tgctcaacag taccttccag cacgtaggtc
    36301 aaaatttcca tatctttatg cgggtgagtg ccgaagccct gccctgcttc aatcacgtcg
    36361 tcgttaatca cgcgcagcgc ggagaagccc ataaagttcg gatcgtaata gttggcaaaa
    36421 gagaaagtat gccaggagtc cagccagcca tgatttgcat gaccacgttc atttgctttg
    36481 cggaagtaga tcatttgtat tcgccccctg aatgatttcg atgggcttag tgtggacccg
    36541 atctgcctgg gatgatagag ggtgaaaatt gacccctctg ttcaaaaaaa ttgaactatt
    36601 cgaggggcca tcaaacttac ttagcgagag ttactgtgga gggagaggct tgctcaaatc
    36661 cgcgttcaag gatttccaga ttggtaagaa cttcagattc cttgacgtaa tttggcgcac
    36721 cgtgggtgat ggtttgatac aacgcatcat aaacgcgccc gtaatcgccc atctccggct
    36781 tcatctcttc tctgaccgtc acgccctcgt cattgacata ctccagcaca ccgaccgaat
    36841 catccgctgc gaatcccggt tcgcccggca taatattagc cttcaggctg gtttcctgct
    36901 ggtcgatacc gtatttaata aacgaacctt tcttaccgtg aacgataaat ttcggataat
    36961 cgattttcac cagatggctg gttttgacga tggctttcag gtcgccataa aacagttgcg
    37021 cttcaaaggt gtcgtcagga ttggctttat tacgcaggct gcggatgtca taagcgacgt
    37081 gatccgggcg accgaacaga gaaataatct ggtccatcgt atgcacacca aggccataaa
    37141 acgcgccatc ctgcggcagc ccaggtttgg tctctgccac cgggcggtaa tagtcaaaat
    37201 ggctttccac ttcaacaatc tctcccaact tgccactttc aatcgctttt ttcgctgtca
    37261 ggaagcagga gtcaaagcga cgattctgat acggcgtgac ggtcagccct ttgcttttcg
    37321 ccaacgcaaa cagctccttc gcctgggcaa gcgtcggggt gaacggtttt tcgaccagca
    37381 catttttccc ggcttccagc gcgcgtttcg cgtactcgaa atggctgtcc gcgtgggtgc
    37441 agacaacaac cagcttaaca tcgggatcgt ttagtacttc gtcgagatcg ctggtgaaat
    37501 ggatatggga ataaatggga gcctgttctt ccggcttcgc atggcgacga aaaatatgcg
    37561 cgacatgcca gctatccttg cggttaagta catacggcag atggtaacgg gtggtgcttt
    37621 tgccgaagcc aataaaggcg cagttgatga ccatgatttc gtccttttta aggtggttat
    37681 ttacacctta gcgcaaagcg gacgtggttc ctactggagt gcgcataacg aacacaagca
    37741 ctcccgtgga taaattgaga acgaaagatc aaaaaaaagc cagcacccgg ctggctaagt
    37801 aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga
    37861 tcgcgagaca ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg
    37921 catttgactc gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca
    37981 aaggaatgag tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc
    38041 agattcacgc ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc
    38101 atatgtggca ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg
    38161 atggagacgt cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg
    38221 ttgccgattt tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc
    38281 tgatgcgaga gatgattgaa atgtgcgaca actggttgcg ggtagatcgc attgaactaa
    38341 ccgtgtttgt cgataacgcc ccggcaatta aggtctataa aaaatacggc tttgaaattg
    38401 aagggactgg taagaagtac gcattgcgta atggtgaata tgtcgatgca tattatatgg
    38461 cgcgggtgaa gtaagatagt gccctttttc tgagatggaa aaagggtgtc attcaaaatc
    38521 gacatacctt cctttaaggt atttatttgc ccaatacata aattgagatt taactcataa
    38581 ttacttccta
//
LOCUS       DAPHDX010000032        37911 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPHDX010000032 DAPHDX010000000
VERSION     DAPHDX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.032"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(100..387)
                     /locus_tag="RH058_001577"
     CDS             complement(100..387)
                     /locus_tag="RH058_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV3137180.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(462..605)
                     /gene="mgrB"
                     /locus_tag="RH058_001578"
     CDS             complement(462..605)
                     /gene="mgrB"
                     /locus_tag="RH058_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV3137181.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            764..1003
                     /locus_tag="RH058_001579"
     CDS             764..1003
                     /locus_tag="RH058_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137182.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1146..1937)
                     /gene="kdgR"
                     /locus_tag="RH058_001580"
     CDS             complement(1146..1937)
                     /gene="kdgR"
                     /locus_tag="RH058_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV3137183.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2114..3487
                     /locus_tag="RH058_001581"
     CDS             2114..3487
                     /locus_tag="RH058_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137184.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIATELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3533..4414)
                     /gene="htpX"
                     /locus_tag="RH058_001582"
     CDS             complement(3533..4414)
                     /gene="htpX"
                     /locus_tag="RH058_001582"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV3137185.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4606..6654)
                     /gene="prc"
                     /locus_tag="RH058_001583"
     CDS             complement(4606..6654)
                     /gene="prc"
                     /locus_tag="RH058_001583"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV3137186.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6674..7372)
                     /gene="proQ"
                     /locus_tag="RH058_001584"
     CDS             complement(6674..7372)
                     /gene="proQ"
                     /locus_tag="RH058_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV3137187.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7469..7966)
                     /gene="msrC"
                     /locus_tag="RH058_001585"
     CDS             complement(7469..7966)
                     /gene="msrC"
                     /locus_tag="RH058_001585"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV3137188.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8096..9379
                     /gene="yebS"
                     /locus_tag="RH058_001586"
     CDS             8096..9379
                     /gene="yebS"
                     /locus_tag="RH058_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV3137189.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9348..11981
                     /gene="yebT"
                     /locus_tag="RH058_001587"
     CDS             9348..11981
                     /gene="yebT"
                     /locus_tag="RH058_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV3137190.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12061..13500
                     /gene="rsmF"
                     /locus_tag="RH058_001588"
     CDS             12061..13500
                     /gene="rsmF"
                     /locus_tag="RH058_001588"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV3137191.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAAQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPTYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13618..13854
                     /locus_tag="RH058_001589"
     CDS             13618..13854
                     /locus_tag="RH058_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV3137192.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13959..14150
                     /locus_tag="RH058_001590"
     CDS             13959..14150
                     /locus_tag="RH058_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV3137193.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14151..14807)
                     /gene="pphA"
                     /locus_tag="RH058_001591"
     CDS             complement(14151..14807)
                     /gene="pphA"
                     /locus_tag="RH058_001591"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV3137194.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15203..15544)
                     /locus_tag="RH058_001592"
     CDS             complement(15203..15544)
                     /locus_tag="RH058_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV3137195.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15557..16429)
                     /gene="copD"
                     /locus_tag="RH058_001593"
     CDS             complement(15557..16429)
                     /gene="copD"
                     /locus_tag="RH058_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV3137196.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16433..16807)
                     /gene="yobA"
                     /locus_tag="RH058_001594"
     CDS             complement(16433..16807)
                     /gene="yobA"
                     /locus_tag="RH058_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV3137197.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16946..17176
                     /gene="holE"
                     /locus_tag="RH058_001595"
     CDS             16946..17176
                     /gene="holE"
                     /locus_tag="RH058_001595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV3137198.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17278..17934
                     /locus_tag="RH058_001596"
     CDS             17278..17934
                     /locus_tag="RH058_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV3137199.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17958..18620
                     /gene="exoX"
                     /locus_tag="RH058_001597"
     CDS             17958..18620
                     /gene="exoX"
                     /locus_tag="RH058_001597"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV3137200.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18617..20677)
                     /gene="ptrB"
                     /locus_tag="RH058_001598"
     CDS             complement(18617..20677)
                     /gene="ptrB"
                     /locus_tag="RH058_001598"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV3137201.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20886..21545)
                     /locus_tag="RH058_001599"
     CDS             complement(20886..21545)
                     /locus_tag="RH058_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV3137202.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21872..22228)
                     /locus_tag="RH058_001600"
     CDS             complement(21872..22228)
                     /locus_tag="RH058_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV3137203.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22295..22585)
                     /gene="yebG"
                     /locus_tag="RH058_001601"
     CDS             complement(22295..22585)
                     /gene="yebG"
                     /locus_tag="RH058_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV3137204.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22719..23897
                     /gene="purT"
                     /locus_tag="RH058_001602"
     CDS             22719..23897
                     /gene="purT"
                     /locus_tag="RH058_001602"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV3137205.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23953..24594)
                     /gene="kdgA"
                     /locus_tag="RH058_001603"
                     /gene_synonym="eda"
     CDS             complement(23953..24594)
                     /gene="kdgA"
                     /locus_tag="RH058_001603"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV3137206.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24631..26442)
                     /gene="edd"
                     /locus_tag="RH058_001604"
     CDS             complement(24631..26442)
                     /gene="edd"
                     /locus_tag="RH058_001604"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV3137207.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26677..28152)
                     /gene="zwf"
                     /locus_tag="RH058_001605"
     CDS             complement(26677..28152)
                     /gene="zwf"
                     /locus_tag="RH058_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV3137208.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28490..29359
                     /gene="hexR"
                     /locus_tag="RH058_001606"
                     /gene_synonym="yebK"
     CDS             28490..29359
                     /gene="hexR"
                     /locus_tag="RH058_001606"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV3137209.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29487..30929
                     /gene="pyk"
                     /locus_tag="RH058_001607"
     CDS             29487..30929
                     /gene="pyk"
                     /locus_tag="RH058_001607"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV3137210.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31060..32031)
                     /gene="lpxM"
                     /locus_tag="RH058_001608"
                     /gene_synonym="msbB"
     CDS             complement(31060..32031)
                     /gene="lpxM"
                     /locus_tag="RH058_001608"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV3137211.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32151..33473)
                     /gene="mepM"
                     /locus_tag="RH058_001609"
     CDS             complement(32151..33473)
                     /gene="mepM"
                     /locus_tag="RH058_001609"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV3137212.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33489..34421)
                     /gene="znuA"
                     /locus_tag="RH058_001610"
     CDS             complement(33489..34421)
                     /gene="znuA"
                     /locus_tag="RH058_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV3137213.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34500..35255
                     /gene="znuC"
                     /locus_tag="RH058_001611"
     CDS             34500..35255
                     /gene="znuC"
                     /locus_tag="RH058_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV3137214.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35252..36037
                     /gene="znuB"
                     /locus_tag="RH058_001612"
     CDS             35252..36037
                     /gene="znuB"
                     /locus_tag="RH058_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV3137215.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36184..37194)
                     /gene="ruvB"
                     /locus_tag="RH058_001613"
     CDS             complement(36184..37194)
                     /gene="ruvB"
                     /locus_tag="RH058_001613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV3137216.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37203..37814)
                     /gene="ruvA"
                     /locus_tag="RH058_001614"
     CDS             complement(37203..37814)
                     /gene="ruvA"
                     /locus_tag="RH058_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV3137217.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9080 a         9785 c         9345 g         9701 t
ORIGIN      
        1 cagagtttta atttgaagca gttgcaccat ggcagtgcgt ataatcagga tgaattgagt
       61 aacccttcaa cgacggggtt actcaagatt gaggaaggat tatcgttcgg cgaccagacg
      121 aataatatct tcgtcttcaa ccgatttttg actctcaacg gtctttttat ctgctttctc
      181 aggttcgttt gcttcgcaca aacgacgtaa cagtgcattt tgacgttttt gctgatccag
      241 caacgcctca agcagttcaa tctgttcgtt agtccgagaa ctggcacgat tgataaaaaa
      301 ccacaagatg agcccgataa gaagaaccac caccgataca accaaagacg caatgttcat
      361 cacgcctgaa tttacaactt cgttcatttc accacctctg agtagaggcg ctattctacc
      421 actgctggag aggaagaaaa tctagtgctg aaaaaatgat atcaccacgg gataaactgg
      481 ttaatggcac aaattccgct gaaaaattgt acatcctgat cgcacatcat gttgaatacc
      541 tgcgcccaaa gcagcaagca agccaacacc acgacaacca gaacgaccca tcgaaacttt
      601 ttcactccaa tctccgcttc acctacctta tgtcattaaa ctagcatgcg ttagttaaac
      661 agcgcctaac tatgtcgata ttcgtgcttt ttcggaatga gtagcttgct tcagcgcata
      721 ttgccgctac gattaagcga acataaaaag agaagaggtt gtaatgcgat tcatcattcg
      781 cacagttatg ctgatagcac tggtatggat aggattatta ctcagcggat atggcgtttt
      841 gattggaagc aaagagaatg ccgcaggatt aggtttgcaa tgtacctatc tgactgccag
      901 aggcaccagc acggtacaat acttgcacac taaaagtggt ttcttgggga taacagattg
      961 cccactgtta cgtaaaagca atatcgtggt cgataatggt taagggtaat aaataaccag
     1021 attctcactc atgagtaaaa tattcttata gcaagcgcgt ttaatataac gcaccttcaa
     1081 ctttattttt ctcatgctaa ataaagaacc tgtctgtttg atattccgac aggttcttac
     1141 tctcatcaga acggatagtc gtgataaccc atttgggcag aaattttgcg cgctgcggta
     1201 tgcaacattg cgacatattc ctgtaaacgc tcttcagaga aacgcaacgt cgggaaggag
     1261 atgctcaaac ctgcaatgac cacgccaaag cgatcaaata ccggtaccgc aatgcatcgc
     1321 agcccttctt cctgctcttc gttatcttcg ccatacccct gctcgcgcac ctggtccaga
     1381 acgggtaata acgcttctgt actggtgatg gtccgctcgg tactgcgttt atactccacg
     1441 ccctcaagaa tttgcttcac ttcatcgcga tcgcgccatg ccagcagtac cttaccaatc
     1501 gcggtgctgt acagcggatt acgacgccca atccgtgaat acatgcgcaa attgtacata
     1561 gagtcaattt tgtgaatgta aacaatactg tcttcgtcca gtgcgccgag gtggatagtt
     1621 tctttggtca ggcgggagat ctcacgcatc tggatatctg cgctacgaat taaatcgacg
     1681 ttttgtaacg cgcgagcgcc cagttcaaac aatttcaggg tcagggaata tttctccgac
     1741 tccccttcct gcgccacata acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg
     1801 ctttttgaca tcatgacgcg ctgcgacagc tcggttatcc ctatttcgcg ctcttcaccc
     1861 agcgcctgca aaatgccaaa aacttttagc acggaagata cagaatcagg ctgtttatcc
     1921 agatctgcgt tagccattta tcacctcatt gcgagtgttt tataaaaatc agaactgttt
     1981 tttattataa tttcgcacca gggtggtcgc aatccatctt ttgccggtta gttacaattc
     2041 tgcgacatcc accgtgaata tcagtgctag aatcataccc ctgttgatta ttcaccaaag
     2101 atataaaatt cctatgccaa aagttcaggc cgacggcctg ccattgcccc agcgatacgg
     2161 tgcgatatta accattgtga ttggtatttc gatggctgtc cttgacggcg caatcgccaa
     2221 cgtcgccctg ccaacaatcg ccacggacct tcatgccacg ccagccagtt ccatctgggt
     2281 agtgaacgcc tatcaaatcg ccattgtcat ctccctgctc tcgttttcgt ttctgggcga
     2341 tatgtttggc tatcgacgta tttataaatg cggtctggtc gtttttctgt tgtcttcact
     2401 gttctgcgcc ctttctgatt cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt
     2461 cggcggtgca gcgttgatga gcgttaatac cgcacttatc cgcctgatct atccacaacg
     2521 ttttctgggg agagggatgg gcataaactc gtttattgtt gccgtttctt ctgctgccgg
     2581 gccgacaatt gctgcagcaa tcctctccat cgcatcctgg aaatggttat ttttaatcaa
     2641 cgtaccgtta ggtattatcg ccctgcttct ggcgatgcgt tttctgccac ccaatggttc
     2701 tcgcgccagt aaaccccgtt ttgacctgcc cagcgccgtg atgaacgcgt taaccttcgg
     2761 cctgcttatc actgcgttga gtggtttcgc tcaggggcaa tcgctgacgt taattgctac
     2821 ggaactggtg gtaatggttg ttgttggtat tttctttatt cgccgccagc tttctcttcc
     2881 cgtaccgctg ctaccggtgg atttactgcg tatcccgctg ttttcacttt ctatttgcac
     2941 atctgtttgc tctttctgcg cacaaatgct ggcaatggtt tccctgccct tttacctgca
     3001 aaccgtgctc gggcgtagtg aagtcgaaac aggtttactt ctgacaccgt ggccgttagc
     3061 aacgatggtg atggctccgt tggcaggcta tttgattgaa cgcgtacatg caggattgct
     3121 gggtgctcta gggttgttca tcatggctgc ggggcttttt tccctggttc tgctgcccgc
     3181 gtcacctgcg gatatcaata ttatctggcc gatgatctta tgtggcgctg gatttggctt
     3241 gttccagtca cccaataacc acaccattat tacctccgct cctcgcgaac gtagcggtgg
     3301 agccagtggc atgttaggaa cggctcgtct actgggtcag agtagcggcg cggcactggt
     3361 ggcgctgatg ctaaatcagt tcggtgataa tggtacgcac gtttcgctga tggctgcggc
     3421 tattctggca gtgattgctg cctgtgtcag tggtttacgt atcactcagc cacgatccag
     3481 ggcataaaaa aagcgcgtcg atcaggacgc gctttttaat atttacttca tattacttca
     3541 ggtattcacc cgtacgcaga gcttcaattc gtttatccag cggcgggtga gtcatgaaca
     3601 actcactgag cgatttcgac ttaccgttaa tgcagaaagc catcatgctg gttgcttctt
     3661 gcggttcata gctggttttc aggcgctgca gcgcggcaat cattttctcg cgaccaacca
     3721 gttttgccga accggcatca gcatggaatt cacgatgacg cgagaaccac atggtgataa
     3781 tgctcgccag aataccaaac accagttcca gaaccgttgc aaccgcaaag tagatcagcg
     3841 ggttgccgtt gctctcttca ccttcatcac gatttccgcc cataaaaccc gcggcaagct
     3901 gcgccagaat acgggaaata aagataacaa aggtgttcac cacgccctga atcagcgtca
     3961 tggtgaccat atcaccattg gcgatgtggc tgatttcgtg agcaattacc gcctcggctt
     4021 catccgggct catgttctgc agcaaaccgg tgctgacagc aaccagagag gcatcacggc
     4081 gcgcaccggt tgcaaaagcg ttgatgtccg gcgcatggta gatagccact tgcggcatag
     4141 cgatccctgc ctgacgagcc tgggttgcta cagtattaac cagccaacgt tccctttcgt
     4201 tacgcggttg ctcgatcact tccccgccaa cagatcgtaa tgccatccat ttggacatca
     4261 gaagcgaaac gaaggaacca ccaaaaccga acagcaaggc catgatcatc agcccctgaa
     4321 cgctgctcga ctgtatccct gtcaggctca gtaccagccc gaaaacgacc attacggcca
     4381 ggttcgttag caggaagagc gcgattcgca tcataatttt ctttttacct cagtttaaca
     4441 aaacgcaata tgcgataccc acatcgtatg ggttacgcga ctattttcaa gtctggatag
     4501 tgcgtaagtc accagaaaga cacaacttta cattttgtag catctgattt acggcatctt
     4561 gccgctgtta aaaaatcagg cacaatttct tgtgcctgat tgatattact tgacgggagc
     4621 gggttgttcc gcgggtctgg ctttttcaag cttcgccaga tcgagtgcga tattcaccgt
     4681 ctcatccaga taaggatccg gctcctggta atctttaggt agatcatcca gcttcttcaa
     4741 ctccggttta ccttcgcgtt taaagcgttc gttcaaacgc gccagacgcg tcgcatcatc
     4801 ttcattattc tctttctcac gcacagcgta gttcagagaa acgatattgc gcttgtcctt
     4861 catagcgttg aagcgcgcga tatccttcat gatgttctgg aactcaggat ctttcgcgat
     4921 acgcgcatta tgttccttca gcagctccgg cccaaaggcc gttaaatcgc ctgatttaac
     4981 ataagtcgcg gcatcaatgc tatcccacgg cagcgcgtta tcttcgaatt tctcacccgt
     5041 ttccgtttct tcattacccg tcggcatgat gatgtctggc gttacgcctt tacgttgcgt
     5101 actgccgccg ttaacgcgat agaatttctg gatcgtgtac tgcacagaac ccagcgctgg
     5161 ccattcagga cgtaacatct gatcgtaaat acggttcaat gaacggtatt gctgaacggt
     5221 gcctttacca aacgtcggtt cacccacaac cagcgcacga ccgtaatcct gcattgccgc
     5281 ggcaaagatt tctgaagccg aagcactgaa acggtcaacc agtaccacca gcgggccttt
     5341 atagaacacc tgcccgtcgg tatcgctgtc ttcacgaacc ttgccgttgt tatcgcggac
     5401 ctgaacaatg ggacccgcag gaataaacag acctgagagc gataccgctt cggtcaacgc
     5461 cccaccgcca ttgctacgca ggtcgatgat cacactgctg acattctgtt tttccagttt
     5521 ctgcagttga actttgacat cgtctgtcaa acccacatag aagcccggaa tatccagcac
     5581 gccgactttc tctttaccga cggtcttcac cgacatttta accgcgcggt cttcgagacg
     5641 aatacgttca cgggtcaatg ttacagtgcg ggtcttggtc cctttaccag caggtaaaat
     5701 ttccagacga actttactgc ccttcggccc tttaattaag gcaaccacat catcaagacg
     5761 ccagccaatc acgtcaacca tcggcttgcc tgtttgacca acaccgacaa ttttgtcacc
     5821 aacgctgata gctttactct tcgctgccgg accacctgcc accatcgaat tgataacggt
     5881 gtagtcatca tccatttgca gcactgcgcc aataccttcc agcgacaaac tcatttcagt
     5941 gttgaactgt tcggtattac gcggggaaag atagttggta tgcgggtcga tttcacgcgc
     6001 aaacgccgtc attgccagcg agaaaacatc ttcgctgttg gtttgcgcca gacgacgaat
     6061 ggcaaatttg tagcggcgtg tcagggtttc acgaatttct ttatccgttt ttcctgccag
     6121 cttcaggctt aactcgtcga atttgacttt actgtcccac agcgcgttca actcagcctc
     6181 gtttttcggc cagggcgctt tgctgcggtc aaggttataa gtgtcgttgc cggtgaaatc
     6241 catcggcttt tccagtaccg acaaagcgta ctggtaacgc tcaaaacggc gcttttgcgc
     6301 cagattgtag agatcgtaga aaacgtcgag tttgcctgaa cgcagttcat cgcctaactc
     6361 ggttttcttt ttcgcgaact gttcaacatc gcttgccagc agcacgttgt ggctgtaatc
     6421 gagcagattc aggtagcggt caaagatttt ggccgaaaat gcctgatcga ggtcgaactg
     6481 gcgataatga gaacgggtga agcgcgacgt tacgcgctca cttaccgtcg catgctgcgt
     6541 ctcttccttt aataccggaa tttgatcagc acgcgtgata tcttctacag cgaaggtctg
     6601 gcctgctatt gcaagcaggc cagctaacgc ggtaagccta aaaaacatgt tcatgcctgg
     6661 cccggcctcc gtttcagaac accaggtgtt ctgcgcgcac aatcaaagac atacccgaat
     6721 tcagctggac gcggacgccg tctttggtga tttctaatac ggtggcatcc atcgcgtttt
     6781 gacccgcttt caccttcaga gcttgtccga cagtcagagc tgaaatgtca gaaaccgggg
     6841 tgtgctgttc ttcgcgaggt gcttttactg tttttggcgc tttctctacc ggcttttgcg
     6901 cacgaggttt acgttcagcg ccttctttgc ggcgtggcgt ggtcggacgt ggcttgcgtt
     6961 cgcggcgcgg tgcgtcttct ttctcaccag cagctgcggc agcttcgcgt tttttcgctt
     7021 gctgttcagc acgctgtgcc tgaacacgcg ctttcgcttc ttcaagctgc ttgcgagcat
     7081 gctctacatg ttgctcgtcc agctcaccgc atgggttgcc gtcaagatcg acacgcgttg
     7141 cgccgggttt aacaccgtaa agataacgcc agctcgaagt gtagagacgt aaagcggatc
     7201 gtaattgcgt tttgctcagg ttcatttccc cggcaacacg atcgaccaaa tcctgaaaaa
     7261 taccgatttt cagcggacgc gcttcacctt ccgcactgaa acagtgggga aaacgttcgg
     7321 ccagaaacgc gattacttct ttactgctat tcaacttagg ttgattttcc atgaaatttc
     7381 ctgattacaa cggacgtagc caacaagcgc aggcatgaac aggcgtcatt ataatgacgc
     7441 taccagtaaa tgctacgtta tccgttgatt atcctgcgac gctcgcaaag aattttttgt
     7501 aatccgtcgt tgcaagcact ttttcaagct gtgccacaag ctgacgtaag ccttgctcgt
     7561 cctcgtctgt aaagcgaccg aagacggtac tatcgatgtc aagaacacca ataatctgat
     7621 ttttcaccac cagcggcaga acaatttcag aattactcgc cgcatcacag gcaatatgcc
     7681 cgtcaaacgc gtgaacatcc tcgatacgct gcacttgatt gcgggcaacc gcagtgccgc
     7741 acaccccgcg cccgacgggt atccggacac aggcaatttt gccctgaaat ggtcctagta
     7801 ccagtgtatc gtcctcaagc aaataaaaac ctgcccagtt tacgtcagtg agacgctcgt
     7861 ataacaacgc actggtgttc gcaagcgttg ccagaaaact ggtttctcct gccatcagcg
     7921 cgttaaagtc gcgatttaaa tccgcgtaaa attctgtttt gttcattata taatcacttg
     7981 gttgtcttac ctggaactgc cagcctatta aaataagcat taaatgcgtt aatgctcaag
     8041 atcattccca tcatgggtta atattaatgt taattcttat tacatttggc acgtcatggc
     8101 tcttaacaca ccacaaatta cgccgacaaa aaagataaca gtgaggtcaa tcggcgagga
     8161 actgccgcgt ggtgattacc aacgttgccc gcaatgcgac atgctgttta gcctgcccga
     8221 gataaattct catcagagtg cctattgtcc gcgctgtcag gcaaaaattc gcgacgggcg
     8281 cgactggtcg ctaacgcgcc tggcggcaat ggctttcacc atgctgttgc tgatgccgtt
     8341 tgcctggggc gaaccgctgt tgcatatctg gctgttaggt attcgtatcg acgccaacgt
     8401 catgcaaggc atctggcaaa tgaccaaaca gggcgatacg ataacggggg cgatggtctt
     8461 tttctgcgtt attggtgccc ccctcattct ggtgtcctcc atagcatatt tatggtttgg
     8521 taaccgactg ggaatgaatt tacgtccggt actgctgatg cttgagcgac ttaaagagtg
     8581 ggtaatgctg gatatctacc tggtcggcat tggcgttgct tctataaagg tacaggatta
     8641 tgcccatatc caggcgggtg tcggcttgtt ctcttttgtg gcgttggtga ttttaacgac
     8701 ggtgacgttg tcacatctta atgtcgagga gctgtgggag cgattttatc cgcagcgccc
     8761 cgctacgcgt agggacgaga aacttcgtgt ctgtcttggg tgccatttta ccggctatcc
     8821 tgatcagcgt ggtcgctgcc cgcgttgcca tatcccgcta cgcctgcgtc gccgtcatag
     8881 tttgcaaaaa tgctgggcgg cgctgttagc gtcaatcgtt ttgttgttac ctgccaacct
     8941 gttgcctatt tctatcattt atctgaacgg aggacggcag gaagatacaa ttctttccgg
     9001 aattatgtcg ctggcaagta gcaacattgc ggttgcggga atcgtgttta tcgccagtat
     9061 tctggtaccg tttactaaag tgatcgtcat gttcacttta ctgttgagca ttcattttaa
     9121 atgccagcaa ggtttacgca cacgcattct gttactgcgg atggtgacct ggattggtcg
     9181 ctggtcgatg ctcgacctgt ttgtcatatc tttaaccatg tcgctgatta atcgcgatca
     9241 gatcctcgct tttactatgg gaccggctgc gttttatttc ggcgcagcgg taattttgac
     9301 tattcttgct gtggaatggc tggacagccg cttactttgg gatgcacatg agtcaggaaa
     9361 cgcccgcttc gacgactgaa gcgcagatta aaaataaacg ccgtatctca cctttctggc
     9421 tgctgccgtt catcgcgcta atgattgccg gttggctgat ttgggacagt tatcaggacc
     9481 ggggtaatac cgtcaccatc gactttatgt cggcggatgg tattgtcccg ggccgtacgc
     9541 ctgttcgtta tcagggcgtt gaagtcggaa cagtgcagga tatcagcctc agcgacgatc
     9601 ttcgtaagat tgaagtcaag gtcagcatca agtccgatat gaaagatgcg ctgcgcgaag
     9661 agactcagtt ctggctggtg acgccaaaag catcgttggc aggtgtctcc gggctggacg
     9721 ccctcgtcgg tggtaactat atcggcatga tgccaggtaa aggtaaagag caggatcact
     9781 ttgtcgcact cgatacccaa ccgaaatatc gactggacaa tggcgatctg atgatccacc
     9841 tgcaagcccc cgatctcggt tcgctgaaca gcggttcatt ggtctatttc cgcaagatcc
     9901 ctgtgggaaa agtctacgac tatgccatca atcccaacaa gcaaggcgtg gtgattgatg
     9961 tcctgatcga gcggcgtttt accgatctgg tgaaaaaagg tagccgtttc tggaacgttt
    10021 ccggcgttga tgccaatgtc agtatcagtg gcgcgaaggt gaaactggaa agtctggcgg
    10081 cactggttaa cggtgcgatt gccttcgatt cacctgaaga gtcgaaacct gccgaggcgg
    10141 aagatacctt tggtctgtat gaagatctgg cccacagcca gcgtggcgta ataataaaac
    10201 tggaactgcc gagtggggca ggattaaccg ccgactcgac gccattaatg tatcagggac
    10261 tggaagtcgg acagctaact aaactggatt taaatcctgg tggtaaagtc actggggaaa
    10321 tgacagttga tcccagcgtc gttaccctgc ttcgtgaaaa tacccgcatc gaattacgta
    10381 atccgaaatt atcccttagc gatgccaatc tcagcgccct gctgactggc aaaaccttcg
    10441 agttggtacc cggcgatggc gagccacgca aagagttcgt tgttgtgcca ggcgaaaaag
    10501 cactgctgca tgaacctgat gttctgacgc tgaccctgac cgcgccggaa agttacggta
    10561 ttgatgctgg tcagccgctc attcttcacg gcgtgcaggt aggccaggtt atcgatcgta
    10621 aactcaccag caaaggcgtc acctttaccg tcgccatcga gcctcagcat cgagaactgg
    10681 taaaaggcga tagcaaattt gtcgtcaaca gccgtgtcga cgtgaaggtg gggctggatg
    10741 gcgttgagtt tctcggtgcc agcgcctcag aatggattaa cggcgggata cgtattctgc
    10801 cgggcgataa aggcgagatg aaagccagct atccactgta tgccaatctg gaaaaagcgc
    10861 tggagaacag ccttagcgat ttacccacca caaccgtgag tttgagtgca gagacgctgc
    10921 cggatgtgca ggcaggatcg gtagtgctct accgtaaatt tgaagttggt gaagttatta
    10981 ccgtccgtcc gcgagctaac gcgtttgata tcgatctgca tattaagccg gagtatcgca
    11041 accttctgac cagcaatagc gtgttctggg cagaaggcgg ggcgaaagtt cagctgaatg
    11101 gtagtggcct gaccgtacag gcatccccgc tctccagagc attaaaggga gccattagct
    11161 tcgataacct cagcggtgcc agcgccagtc agcgtaaagg cgacaaacga attctgtatg
    11221 cttccgaaac agcggcccgt gcggttggtg ggcagattac gcttcacgct ttcgatgccg
    11281 gaaaactggc ggtcgggatg ccaattcgct atctcggtat tgatatcggg caaatccaga
    11341 cgctggatct gattaccgcg cgcaatgaag tacaggcaaa ggcggtgctc tatccggaat
    11401 atgtccagac ctttgctcgc ggtggtacgc gcttctcagt ggtcacaccg caaatttcgg
    11461 cagctggcgt tgagcatctt gatactatcc tccagccgta tatcaacgtc gaaccaggcc
    11521 ggggcaatcc tcgccgcgac tttgaattac aagaggccac cattactgat tcgcgttacc
    11581 tggatggctt aagcattatt gttgaagcgc cggaagccgg ttcgttaggc atcggtacgc
    11641 ctgtgctgtt ccgtggtctg gaagtcggta cggttacagg aatgacgctg gggacattgt
    11701 cagatcgcgt gatgattgcg atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca
    11761 attccgtctt ctggttggca tcgggttaca gtctggactt tggtctgacg ggcggcgtag
    11821 tgaaaaccgg cacctttaac cagtttatcc gtggcggcat cgccttcgcc acgcctccgg
    11881 gtacgccact ggcaccgaaa gcccaggaag gcaaacactt cctgttgcag gaaagtgaac
    11941 cgaaagagtg gcgtgaatgg ggaactgcgc ttcccaaata atgcccactg ctccggcgtg
    12001 cctgcgccgg agcgtttatg ctaaactgcg cgcctgtttt tttgccagtg gtacatgctc
    12061 gtggcccaac acaccgttta tttcccggac gcctttctga cgcaaatgcg cgaagcgatg
    12121 ccttcgacgc tctcttttga tgattttctt gccgcctgtc agcgcccgtt gcgccgcagc
    12181 attcgcgtta atacgctgaa aatctccgtt gctgatttcc tgcaattaac cgctccttat
    12241 ggctggacgc ttacgccaat tccgtggtgt gaagaaggtt tctggattga acgcgacaat
    12301 gaagatgcat tgccattggg tagtaccgcc gagcatttaa gcggcctgtt ttatattcag
    12361 gaagccagtt caatgttgcc cgtcgccgcc ttgtttgctg acggtaatgc agcacagcgg
    12421 gtgatggatg tcgctgctgc gccaggctcc aaaacgacgc aaattgccgc gcggatgaat
    12481 aacgaagggg caatccttgc caatgagttt tccgccagtc gggtaaaagt gttacatgcc
    12541 aatatcagcc gctgtggcat cagtaatgtt gcgctcacac attttgatgg ccgcgtgttt
    12601 ggtgtggcag tgccagaaat gttcgatgcc attttgctgg acgctccctg ctctggcgaa
    12661 ggcgtggtgc gtaaagatcc cgatgcgcta aaaaactggt caccagaaag caatcaggaa
    12721 atcgcagcta cacaacggga gcttatcgac agcgcctttc atgcattacg tcctggtggt
    12781 acgctggttt actcgacctg taccttaaac agggaagaaa acgaagccgt ttgcatgtgg
    12841 ctgaaagaga cttaccctga cgcagtagag tttttaccac ttggcgagct cttccctggt
    12901 gcaaacaaag cgctgaccga agaaggcttt ttgcatgttt tcccacaaat ttacgactgc
    12961 gaaggcttct tcgttgctcg tctgcgtaaa actcaggcca ttcccgcctt acccacccca
    13021 acatacaaag tgggcaattt tccgttcagc ccggtgaaag atcgcgaagc tggacaaatt
    13081 cggcaggcgg ctgcaggtgt tggcttaaac tgggatgaaa acctgcgcct ctggcagcgt
    13141 gacaaagaac tgtggttgtt cccggtgggc attgaagccc tgatcggtaa agtccgattt
    13201 tctcggttgg ggattaaact tgccgagacg cacaacaaag gttatcgctg gcagcatgaa
    13261 gcggttattg ctcttgcctc acccgataat atgaatgcat tcgagctgac accgcaggaa
    13321 gcggaggagt ggtatcgcgg gcgcgatgtt tacccgcaag ccgcgccagt ggcggatgat
    13381 gtattggtta ctttccagca tcaaccgatt ggtttagcca aacggattgg ttcgcgattg
    13441 aaaaacagct atccgcgtga actggtgcgc gatgggaaac tttttaccgg taacgcctga
    13501 cagcgcacaa aaaaagcgca ctttttgact ggcacattcg gcggcctcaa ctaggctgaa
    13561 aaatggtgcg atcggactgg tcgtaccaca atcggcagct aaatggagag cacgaagatg
    13621 aaaaccagtg tgcgcatagg cgcttttgaa atcgacgacg gcgaattaca cggtgaatcg
    13681 ccgggtgatc gaacgttaac cattccttgt aaatctgacc ccgatttatg tatgcaactc
    13741 gatgcctggg atgctgaaac cagtatccct gccctgctaa atggcgaaca ctctgtcctt
    13801 taccgtaccc gttacgatca acaatctgat gcctggatta tgcgtcttgc ctgatccaaa
    13861 aagaacccgt cggcatggcg ggttatttgt cctggttatt cccccgttgt aaaatctctc
    13921 ctaaacttaa cggtacggca ccacacttcg gggatgaaat gttcgcgctg gtactttttg
    13981 tttgctacct ggatggcggt tgtgaagata ttgttgtgga tgtctacaac acggaacagc
    14041 agtgtcttta ttctatgagc gatcaacgga tccgccatgg cggttgtttt ccgattgagg
    14101 attttataga tggtttctgg cgacctgcac aggagtacgg tgatttttaa ttattgcaat
    14161 tgcacaagag tcagttcgcc cccaaagaca gcaccggtat caatataatg caggttgcca
    14221 atatccacgc gatgtcgcaa cggtgtatga ccaaaccaga aatgatcagc acctgtaatt
    14281 ccctgccctt tttggcgttc acctaatcgc gagcggctcc acaagacctg atgcaaatca
    14341 acgtcctttt gccattcata aacatcatct ggataatcgg catgagcaat aacatgtttg
    14401 ccggtacgac tgtgtacttc aagaataaag ggcaaatgct gacatttttc cagcgccgtt
    14461 ttcgcttgtt tctgttgatt atctgccagc gcaataaacc agtcgccgcc attcatcaac
    14521 cacaaagaca tctgctgaga tgccagcgca tccatcgcca tctgttcatg attgcctctt
    14581 accgcacgaa cccaatgttg ttccagtaac tgcagacaac gtaaactttg cggcccacga
    14641 tcgataacgt ctcctactga gataagtaaa tctcgccacg gatcaaaacg acaatgccat
    14701 aatttgcggc gcaactgctc aagacaaccg tgtatatcgc cagaaagcca gatatgtcgc
    14761 cattgatgac ccgcaattct ctgataaacg ggcgcaggct gtttcatcaa tattttcctc
    14821 ccgcgctaaa gatcacataa tcttaacaag aatgttaaaa aacgctggac tcagacagta
    14881 gagtgtgtgt tatggttgac tataaagtca gcgaaggaaa tgcttctggc ttttaacaga
    14941 taaaaagaga ccgaacacga ttcctgtatt cggtccaggg aaatggctct tgggagagag
    15001 ccgtgcgcta aaagttggca ttaatgcagg cttagttgcc ttgcccttta agaatagatg
    15061 acgacgccag gttttccagt ttgcgtgcaa aatggtcaat aaaaagcgtg gtggtcatca
    15121 gctgaaatgt taaaaaccgc ccgttctggt gaaagaactg aggcggtttt tttattggaa
    15181 atcaaaaggc tattttaggt aattaacaga gtttttcagc tcgttctata aacggtgcca
    15241 gactcatttt ttcgccggga ttgttaggat catcaatctg aatcaccgaa atgggttggg
    15301 ctttagtctt cccactggca acttcctttt gtgcgatatc gtttaaagga tactgcacga
    15361 gggtacttgg gttaatgaca tacaaagcat tacccggtct gcaagtcagc atcacctctt
    15421 cgcgattaaa cgcccatttg tctttaccca cttcaaaacg actgacggta atcacctgcg
    15481 gtgcagccag cgccgctgca gaactggtga gtaacagaaa cgtcagaata ctttttttca
    15541 tcattttcgt aatccatcaa aaaggttcga gagtcgcaag caggctgatg atcaccagca
    15601 ctaccgcacc tatcgcccat tcgagtttag tcatccactc aaaccagggt gccgcacgat
    15661 cttcatcctg cctcatgcgc ggtacaagaa cataacgatt agccagcgca atgaccacca
    15721 taatcatcac caggatggct ttgagcaata agagttggcc ccagtaagtc gtaagcgtgg
    15781 ggggaaatcc ggtaatcaac aatgcattaa gcacgccgct tgccagtacg ccgatcaccg
    15841 caaaatgccc gcaccaggaa aaacgcatca gcgcctgaat ggcctggtgt cgccaacggc
    15901 ctttgatgag ttgcatacac cagagcaccg gcagtaatcc cccaaaccac gccgccgcac
    15961 aaatcaggtg aatcgcgtga ttagtctgat ggattttcgc cgttacccct tcattcatcg
    16021 tcgcatgccc cactcccgcc agcagaataa attgcgcggt agtgagcata aacagcaaac
    16081 gtgacacatt ccgcggttgc ataagggcga cgatcaacgt tacgagggcg agaacaattt
    16141 gccataacca gacgccacca aactgcgttt gcaaaacagc ctgccaaatg tttggcgaaa
    16201 atacatctgc ccatcccgtt cccatcaacc cgccctgaac ggcgagcatt gcagtggcac
    16261 tgatcaaact ccagacggca gcgtgctgtt gtaaacgcag aaaacgcttc gccagcaagc
    16321 gacgaatcgt taagggggcc agccaggcac cgtacattgc gaagccaaag accagcatca
    16381 gcgaggtgaa atggataaat cgcagcgcga tccaggtaaa cgccagcatg atttatttca
    16441 cgctaaaggt gtagtgtcct ttcgttttat gcccgtccac cgaaacaaca tgccagtcaa
    16501 cgatgtaggt tccgggtttc agcgaatcgg ccagtggaac aatcagttgc ttttggtcct
    16561 gctcatttcg cttcgccggt aatgttttaa tattttcgtt ttttggcccc gtgatttttg
    16621 caccactgaa tcctgtttca acaccttccg agaagtttaa agtgattgcc tgcggtgcag
    16681 ctgtcacttg cgcgtttgcc gcaggatact gatgcgttaa atgcgcatgt gcccagacgg
    16741 aaggtgtcac caacgaagtg gtcaggattg ccagcgcgta gcgaagggag cgtgcagttg
    16801 aagtcatatt atctattcct ttttgtaata acttttttac agagcataac cttgtctaat
    16861 gtctgagtcg aggatcatca attccggctt gccatcctgg ctcactctta gtaacttttg
    16921 cccgcgaatg atgaggagat taagaatgct gaagaatctg gctaaactgg atcaaacaga
    16981 aatggataaa gtgaatgtcg atttggcggc ggccggggtg gcatttaaag aacgctacaa
    17041 tatgccggtg atcgctgaag cggttgaacg tgaacagcct gaacatttgc gcagctggtt
    17101 tcgcgagcgg cttattgccc accgtttggc ttcggtcaat ctgtcacgtt taccttacga
    17161 gcccaaactt aaataaaact tatacagagt tacactttct tacataacgc ctgctaaatt
    17221 atgagtattt tctaaaccgc acccataatt tgcagtcatt ttgaaaagga agtcattatg
    17281 tcgttctgga aagttgcagc agcgcaatat gagcctcgta aaacctcact caccgaacag
    17341 gtagctcatc atctggagtt tgtgagggcc gccgccaggc aacaatgtca gctgttagtt
    17401 tttccatcgc tttctttact ggggtgtgat tattcgcgac gtgcccttcc tgccccaccc
    17461 gatctctcac tgttagaccc gctttgttat gccgcaacga cctggcgaat gaccatcatt
    17521 gccggccttc ccgttgaata taacgatcgc tttattcgtg gaattgcggt gttcgcaccg
    17581 tggcgaaaaa cgcctgggat ctaccatcag agtcatggtg catgtctggg tcgtcgttcc
    17641 agaaccatca cggtggtcga tgaacaaccg gaaggtatgg atatggaccc gacctgttca
    17701 ctgtttacta cagggcaatg tctgggagaa cccgacctgc tggcgtcggc ccgtcgctta
    17761 caattttttt cacatcagta ctcgattgcc gtgctgatgg caaatgcccg tggtaacagt
    17821 gcactatggg atgaatatgg tcgtctcatc gttcgcgccg atcgtggttc attattgtta
    17881 gtcggtcagc gttcatcaca gggttggcaa ggcgatatca ttccattacg ctaggctttt
    17941 tcggcctgga gcatgccatg ttgcgcatta tcgatacaga aacctgcggt ttgcagggag
    18001 ggatcgttga gattgcctct gttgatgtca ttgacggaaa aatcgtcaac cccatgagcc
    18061 acctggtgcg ccccgatcgt cctattagtc cacaagcgat ggcgattcat cgcatcaccg
    18121 aagccatggt cgccgataaa ccgtggattg aagatgtgat cccacactat tacggtagtg
    18181 aatggtatgt cgcgcataac gccagctttg accgccgcgt actgcctgag atgcccggtg
    18241 agtggatttg cactatgaaa ctggcccgtc gtttgtggcc tgggatcaag tacagcaata
    18301 tggcgttata taaaacacgc aagctcaatg tacagacgcc gccgggcctg catcatcacc
    18361 gcgcgttgta tgactgttat atcaccgccg cgttgcttat cgatattatg aacacctccg
    18421 gctggacggc agaacagatg gccgatatca ccggacgtcc gtcgttgatg acgaccttca
    18481 cctttggcaa ataccgtggc aaagcggttt ccgacgttgc cgaacgcgat ccgggctatc
    18541 tgcgctggtt atttaataac ctggacagca tgagcccgga gctgcgttta acactgaaac
    18601 attatctgga aaatacttag tccgcaggcg tagcgggtaa tgttccctgc gccagcgcga
    18661 ccagaaaagc atattccatc gctacgcctt cgtacgattt aaagcgacca gatttaccgc
    18721 catggcctga gtccatgtcg gtacagagca ataaaagatg gtcatcggtt ttcagctcgc
    18781 gcaatttagc gacccatttt gccggttccc aatattgcac ctgagaatcg tgcaaaccgg
    18841 tcgttaccag taaatgcgga taagcctgtg cggtgacgtt gtcatacggg ctgtagcttt
    18901 tcatgtactc gtaatattgc ggatcctgcg ggttacccca ctcttcaaac tcaccagtgg
    18961 taagaggaat tgattcatca agcatcgttg ttacaacatc aacaaacggt acctgggcga
    19021 taacgccgtg gaataattcc gggcgttgat taattgcaac gcccatcaac atgccccccg
    19081 cactcccgcc catcgcataa caaagcgaag gagagccata gcccagtttt aacaatgcat
    19141 cgcaggcatc aagataatca ttaaacgtat ttttcttctt cagaaatttt ccgtcttcgt
    19201 accattgttg ccccagctca ccaccgccgc gaacatggac aatggcgtag acaaagccac
    19261 gatctaacaa actcaagcgg ctaaaactga aatcggcatc aatacttgcg ccgtaagaac
    19321 catagccata caccagcaac gggttgtgtc ctttgcgaaa atgtttgcga tggtagacca
    19381 acgaaaccgg aacttcgacg ccatcacggg cgactatcca caggtgttca ctgcggtaat
    19441 tcgccgcatc aaaaccgggc acttctgttt gttttaatac acgacgctca ccggtatcca
    19501 tatccagttc aaacaaagtg tctggtgtgg tcatggaaga ataaccataa cgcaatcgcg
    19561 cggtttcagg ttctggattg taggcaatcc aggtcacata ggctggatca tcaaaggcaa
    19621 taccaatgac ttcccgggtc ttgcggttaa tttggcgcaa actggttaac ccgcgctgac
    19681 gctcttcaac caccagccag tcggtaaaca gcgtaaaccc ttccagcatg atgttttcgc
    19741 gtggcggaat taactcttcc cactgttgct catcacgcat acgggtacgg tataagccaa
    19801 agtttttgcc gtggcggttg gaacgcagat aaaaacgatg ctggtagtga tcaaggctgt
    19861 attcgtgatc tttgcggcgc ggcagaaaaa caaacggctc ggcatcggcc atttccgcgt
    19921 ccagcaggcg aacttcactg gtggtggcgc tggccaaatg aatgactaca tagtgcttcg
    19981 aggtcgtttt atgcaggctg acgtaatagg tatcgtcttt ttcttcgtag atcagtttat
    20041 cttgcgatgc tggcgtaccg atggcgtgac gccagacctg ataaggcagc agcgtcaccg
    20101 gatgcttgcg aacatagtag aaagtccagg agtcatttgc ccagacaaag ctgggttcaa
    20161 cgttatccag cagttccggg taccagttac ctgtttccag attacgaaaa cgaatgccgt
    20221 actggcgtcg ggaaagaaaa tcttctgcca gcgccataat ggtgttatcg ggcgtaatcg
    20281 ccattccgcc catcgaataa aactcactat gagccgcgcg cttgttggca tcgagcaata
    20341 tttcccactc gtcccactct tcactgaacg ccgattgacg ctggtagata gcatattcac
    20401 agcctggttc ataaatatgc cgatagcggt agccattttt gatgtagggc gcagaaactt
    20461 ctcgttgcgg aatgcggtcg atgatttcct ttaagatgcg atcctgcaag gcttgttgtg
    20521 aggccatcac ccgatgaccg taactatttt cttgttgcag gtagtccagg acttctggct
    20581 gagaacgcgt atcgtcccgc agccagtagt aattatcgat gcgcgtatcg ccatgaagcg
    20641 tcatggcgtg gggaatgcgg gcggcttttg gtagcatgtt attgttcttt ctggttgaaa
    20701 catcctataa gggtggcaaa actcaccggg gatgcaagcg aaacagggga gtcattgctt
    20761 agatgatgac aggtaatggc gcggatatcg aatgttatgc agacagagaa aatcagtctg
    20821 ttcaaatggc tgtgcggttc tggatagccc gaaatattca acttcagact atccagagag
    20881 cggaattatt ccgccaaagt gcgtttttgc tgttcgagat cgcgttcaat gccgtcacga
    20941 acatcctggg ggattttcag cgcgtcaccc agtgcattca ggtaactgcg ttccataaaa
    21001 tggtcaatat caatagccgc gcaactcagg aaatagattt ccagcgcctc ttcttcattg
    21061 cggaccccgg tagccaggcg ttgtggatcc agcggttgtt cgattgcctg ctcaatgagt
    21121 acacgcccct gctcttccac gccggcttca cgcaattgct ggtcgattgc cgcacgttct
    21181 ttggcatcaa tatgaccatc acttttagcg gcaaagacca gcgcaaggat caaacgttct
    21241 gtacgctcat ccagcggcgt actttgcgcg ccaaactgcg gttcgtcctg atgcgccgcg
    21301 cgaattttat ctttgtattt attccacagc accgtacccg ctaccgctcc gccgccaacc
    21361 agtaacgcgt tggtgccata tttcgtaaga agtttacgtg ctgatttatt tgcgaccagc
    21421 agcccagcca gtccgcctaa tgcgcctggc actaacagtt tgcccaatcc ctgatccgca
    21481 gacgaggagg tagaagaact gctttgccca agcagggatt gcagttgatt taaccagtta
    21541 gccatatttg ctcctcaata accattttta gtgcctgtag catagcggat gaggatgtca
    21601 gaaaatgtat atcgagacaa aagatgcgca aataagcggg gcggtaggcc gttattcaaa
    21661 gaaattcgcg ccaggtaagg tatttactgt atatcaggtg ataagggtat tttaggtgaa
    21721 agtagtttgt cgttttgagt ggcattgttt gatgctgatt gcccgatgcg acgctgttgc
    21781 gtcttctcag gcctacgatt gtggtgcgtt ctggggcgga caaggcactc gcgccgcatc
    21841 cgccaacggt caatcgaatt atcagaagcc attaacgccg ctgatattcc gccattcccg
    21901 ctttgcagtc cacgctgacc tggtaatgaa tatcggcact tttaccacgc acggttagcg
    21961 gtactgacca tttatcatct ttaccctgaa tgtcctgcaa actgacccaa gccacgggat
    22021 cggcctgacc gacaattttt tgatcatctg cccagcgcgc cacgcgattt tgttgataat
    22081 cacgttttac gctcgcggca attccggcag catccagacc ttcacacttt gggaaagtga
    22141 ccgacttgct ggtttcatta ttggcagcga aaactgatgc gcaggcagaa accaacaaca
    22201 gccctaaaaa cgcccctctt tttttcatgt ttttctccat agcacaatga ttcaggagaa
    22261 agcatggtac aaattgtcag gagcgcaagt tgcttcaggc cgcgtgtgag gcatcttcct
    22321 cttcggattc agcaccgacg acctgtgggg acggtaattt gccggttttc agaatggtgc
    22381 tcagcacatc tttttgttct gccagccata gcgaaagggc ttcacgttgc tcgtcttcca
    22441 tttgcactgg agaatttgtc agccaggtgt caagcagatc cgccgtatca agcattttgt
    22501 cataagcatc ggcttccttt ttgctggtaa acgacatttt ctcttcgccc tcacgaatga
    22561 ctacgtattt aacttcaacc gccatttgca gcctctcata ataactgtga ttttatacag
    22621 tatatttctt ttcggttgag aaatcaacat cagcaataaa gacatacgca aacgttttcg
    22681 tttatactgc gcgcggaatt aatcagggga tattcgttat gacgttatta ggcactgcgc
    22741 tgcgtccggc agcaactcgc gtgatgttat taggctccgg tgaactgggt aaagaagtgg
    22801 caatcgagtg tcagcgtctc ggcgtagagg tgattgccgt cgatcgctat tccgacgcac
    22861 cagccatgca tgtcgcgcat cgctcccatg tcattaatat gcttgatggt gatgcattac
    22921 gccgtgtggt tgaactggaa aaaccacatt atatcgtgcc ggagatcgaa gctattgcca
    22981 ccgatatgct gatccaactt gaagaggaag gactgaatgt tgtcccctgc gctcgcgcaa
    23041 cgaaattaac gatgaatcgc gagggtatcc gtcgcctggc ggcagaagag ctgcagctgc
    23101 ccacttccac ttatcgtttt gccgatagcg aaagcctttt ccgcgaggcg gttgccgcca
    23161 ttggctatcc ctgcattgta aaaccggtga tgagctcttc cggcaagggg cagacgttta
    23221 ttcgctctgc agagcaactt gctcaggcat gggaatacgc tcagcaaggc ggtcgcgccg
    23281 gagcgggccg cgtaattgtt gaaggcgtcg ttaagtttga cttcgaaatt accctgctaa
    23341 ccgtcagcgc ggtggatggc gtccatttct gtgcaccagt aggtcatcgc caggaagatg
    23401 gcgactaccg tgaatcctgg caaccacagc aaatgagccc gcttgccctt gaacgtgcgc
    23461 aggagattgc ccgtaaagtg gtgctggcac tgggcggtta tggtctgttt ggtgtcgagc
    23521 tgtttgtctg tggtgatgag gtgattttca gtgaggtctc ccctcgtcca catgataccg
    23581 ggatggtgac gttaatttct caagatctct cagagtttgc cctgcatgta cgtgccttcc
    23641 tcggacttcc tgttggcggg atccgtcagt atggtcctgc agcttctgcc gttattctgc
    23701 cacaactgac cagtcagaat gtcacgtttg ataatgtgca gaatgccgta ggcgcagatt
    23761 tgcagattcg tttatttggt aagccggaaa ttgatggcag ccgtcgtctg ggtgttgcgc
    23821 tggcgaccgc tgagagtgtt gttgacgcca ttgaacgcgc gaagcacgcc gccggacagg
    23881 taaaagtaca gggttaaacc cggcaaaaaa acgctacaaa aatgcccgat cgtagatcgg
    23941 gcgttttgac ttttacagct tagcgccttc tacagcttca cgcgccagct tagtaatgcg
    24001 gtcgtaatcg cccgcttcca gcgcatctgc cggaaccagc caggaaccac cgatgcacag
    24061 cacgcttttc agcgccaggt agtcacggta gttagccgga gaaataccac ccgtcgggca
    24121 gaaacggacc tgggagaacg gacccgcgat cgcctgcagg gctttcacgc cgccgttagc
    24181 ttcagccggg aagaatttga actctttcaa accgtagtcc atacccagca tcagttcgga
    24241 aacagtgctg atccccggaa ttagaggaat agtcccttcg gtagcagctt tcagcagcgg
    24301 ctcggtcaga cccgggctaa ttgcgaactg tgcacccgct tcagtgactt ctgccagctg
    24361 ctgtggattc agcaccgtac cggcacccac aatcgcttca ggcacttctt tggcgatagc
    24421 acggatagcg tcaactgcac actcggtacg cagagtcact tccagaacgc gcaccccacc
    24481 agcaaccaac gcttttgcca tcggcactgc gtgttccagt ttttttacca cgataaccgg
    24541 tacaaccggg ccggtggtca ggattgattc tgcacttgtt ttccagtttt tcatcagagt
    24601 tttctctcgc ctgattacaa atttgtcgtc ttaaaaagtg atacaggttg cgccctgttc
    24661 ggcaccggac agtttttcac gcaaggcgct gaataattca cgtcctgtcc ccacgcgtga
    24721 cgcgctcagg tcaggaatgt gcggttcgcg agcagccagt tccgcttcgt ctaccagcag
    24781 cgtcagttcg cctgtctgtc cattcacacg aatgatgtcc ccgtcgcgca cttttgccag
    24841 cagcccgcca tcgtaggctt ctggtgttac gtggatagct gacggcactt tacctgaagc
    24901 gccggagagt cgtccatcgg taactaacgc aattttgaaa caccggtcca ataatacacc
    24961 aagtggcggc atgagtttat gtaattctgg cattccgttc gcttttggcc cctgatgacg
    25021 gacaacaacg acacaatcgc ggtccagcaa acccgcttca aaggccggca taacgtcatg
    25081 ctggctttca aaaacaaccg ctggcgcttc aatcacctgg ttctcaaccg gcacggcaga
    25141 ggttttcata accgcacggc ccaggttacc gcttaacact tttgtcccac catgatgaga
    25201 gaaaggttgt tcgaaggaag cgatcacatt gctgtcgagt gatttttccg ccccttcccg
    25261 ccagtccagt tcaccattat tcagccatgg ttcaagggta taacgagaca gaccaaagcc
    25321 tgccaccgta ttgacatctt catgcagcag gcctgctttg agcagttcac gcaccagaac
    25381 cggtacgcca cctgccgcct ggaagtggtt aatatcggcc ggaccgttcg ggtagagacg
    25441 tgccatcagc ggtacaacat cagaaaggtc ggagaagtca tcccagttaa tctgaatacc
    25501 ggccgcgcgc gccatcgcca ccaggtgcat ggtgtggtta gtggaaccac cggtcgccag
    25561 cagagcaaca ataccgttca ccaccacttt ctcatcgatc atcttaccga tcggcatcca
    25621 ttcattacca ttaccggtca tgcgtgtaac ctgacgcgca gcggcagcgg tcaaagcatc
    25681 gcgcagcgga gaatccggat gaacaaaaga agagcctggc aactgcatcc ccataaattc
    25741 caccaccatc tggttggtgt tggcagtacc gtagaaagta catgttcccg gcgcatggta
    25801 agacgcggct tctgactcca gtaaggccat gcggtccact ttaccttcgg cataaagctg
    25861 gcgaatacgc actttttctt tatttggcaa accgcttgcc atcggtccag acggcacaaa
    25921 caccgcaggc aaatgaccaa acgacagggc tgccatcgtc agacccggga caatcttgtc
    25981 gcacacaccg aggaacagag caccatcaaa catgttatgg gacagcccga ccgccgcaga
    26041 catcgctatc acttcgcggc ttagcagcga caattccatt ccatcctgcc cctgggtgac
    26101 accatcacac atcgccggaa caccgcccgc aacctgacca accgcattcg cttcatgcag
    26161 ggctttacga atgatttctg gatagtgttc ataaggctgg tgcgcggaga gcatgtcgtt
    26221 ataggaggtg atgatggcga tattgttacg caacatgctt ttcaaagagg ctttgtcttc
    26281 tggctggcag gcagcgaaac cgtgtgccag gttaccgcat gccaactgcg aacgatgaac
    26341 ggtcgaagtt ttcgcttgtt ctatccgggc gagataagca gagcgagtct cgcgcgaacg
    26401 ttcaatgatt cgatttgtta cgcgtaacaa ttgtggattc ataaaggctc ctgaaattga
    26461 gttgtcagag caggatgatt cacaacgcgt ttcattcaga ggatttatga ctgaaacgcc
    26521 tgtaaccgga gctcataggg caaaaacgtt tcagtcagtg taataaaaaa agcctcgtgg
    26581 gtgaatccgc acgaggcctg aaagtgtaaa aattgttcta caatctgtgc aagatcatgt
    26641 taccggtaaa ataaccataa aggataagcg cagatattac tcaaactcat tccaggaacg
    26701 accatcacgg gtaatcatcg ccaccgaggc aacgggtccc caggttccgg cctgatacgg
    26761 tttcggcgca tcattgtcca tcgcccacgc ctcagtaatg gagtctaccc atttccaggc
    26821 ttcttccact tcgtcgcgac gaacaaacag tgcctgaata ccacgcatgg tttccagcag
    26881 caaacgttca taggcatccg ccagatgcgt ctgattaaag gtttctgaat agctcagatc
    26941 cagcttggtg atttgcaggt tatgtttgtg gtcaaggcca ggaactttat tcagtacctg
    27001 gatatccacg ccttcatcag gttgcagacg gatagtcagt ttattctgcg gcagatcctg
    27061 ccacgattct ttaaacagat tcagttcagg tgttttgaaa tagaccacga cttcagaaca
    27121 tttggtcggc agacgtttac cagtacgcag gtagaatggc acaccggccc agcgccagtt
    27181 atcaatgtcg acgcggatcg ctacgaaggt ttctgtattg ctgctcttgt tcgcgccctc
    27241 ttcttccaga tatcccggca cttttttgcc ctgggcgaag cccgcagtat attgcccgcg
    27301 tacggttttt tcgcgtacgt tggagcggtc gatgcggcgc agagacttca gtactttcac
    27361 tttttcatcg cggatgctgt ctgcgctcag gtcagacggc ggagacatcg caatcatgca
    27421 gagaatttgc agcaggtggt tttggatcat atcgcgcatc tgaccggctt tatcaaaata
    27481 gccccagcgc ccttcgatcc ccacttcttc tgccacggta atctcaacat gatcaatggt
    27541 gcgattgtcc cagttattca caaacaggga gttagcaaaa cgcagcgcca acaggttcag
    27601 caccgtttct ttaccaagat agtggtcgat acggtaaacc tggcactcct cgaagtattc
    27661 gccaacctga tcattgattt cctgcgaggt cgccagcgac gtccccagcg gtttctccat
    27721 aactacgcgt gccggtttag cattcagttt tgcctcacca agccctttgc aaattgcgcc
    27781 aaaagtgctg ggcggcatgg caaagtagtt aatggtgata cgattttttt gatccagcat
    27841 cgcgccgaga cggctgaatg cagcagtgtc attgacatcg agattacaaa aatccagacg
    27901 tgcactcagg gtgtcccata aaccttcatc aatagtttct ttcatgaaag tttcgagcgc
    27961 ctcacggaca actttggtat aagccgcttt atcccagtca gcacgcccta cgccgataat
    28021 ccgggtgtcc gggttgagct gaccggcttt ttccagttga tacagggaag gcagcaattt
    28081 acgacgcgca aggtcgcctt tcgcgccgaa aatgaccagg tcacaggcct gggctgtttg
    28141 cgttaccgcc atgtcattct ccttaagtta actaacccgg tacttaagcc agggtatact
    28201 tgtaattttc ttacggtgca ctgtactgct tttacgagct tgcgaaaact gtaaacgctt
    28261 atccacccgt gcgattacgg gaaaagcgcg caaagtgcgg caaaactgat aaaaaaatca
    28321 tcgttttcca ttcggtaaaa atctgacact gatcatgtta tgaaaaaaaa taacaacttt
    28381 tttatctgct tttgtcatta acacggcaca caggcgtaat atctgacaaa actgcatttc
    28441 gatttctttc agtgcggaaa tcgtcattac ccgtgagtct ctttacatca tgaatatgct
    28501 ggaaaaaatc cagtctcagc tggaacattt gagcaaatca gagcgcaaag ttgccgaggt
    28561 cattctggct tcgcccgata acgcgatcca ttcgagtatt gctgctatgg cactggaagc
    28621 caatgttagc gaaccgacgg tgaatcgttt ctgtcgcagc atggacacgc gcggttttcc
    28681 tgattttaaa cttcatctgg cacagagtct ggcgaatggc actccctatg ttaatcgcaa
    28741 cgtcaatgaa gatgacagcg ttgaatcata cacagggaaa atatttgagt ccgcaatggc
    28801 aacgctcgat catgtccgtc attcactgga taaatctgcc atcaaccgcg ccgtcgactt
    28861 gctcactcag gcaaaaaaaa tcgccttttt cggattaggc tcttcagccg ccgttgccca
    28921 cgatgcgatg aataagttct ttcgttttaa tgttccggtg gtgtactccg atgatattgt
    28981 gctgcaacgc atgagttgta tgaattgtag cgacggagac gtggtggtgc tgatttctca
    29041 cactggaaga acaaaaaatc tggtcgagct ggcgcagctg gctcgcgaaa acgacgcaat
    29101 ggtgattgcc ctcacctctg cgggtacccc gctcgcccgg gaagcaacgc tggcaattac
    29161 cctcgacgta ccggaagata ctgacattta tatgcccatg gtttctcgac ttgcacagct
    29221 gaccgtgata gatgtgctgg cgacaggatt tactttgcga cgcggtgcaa aattcagaga
    29281 taacttgaag cgggtcaagg aagcgctgaa ggaatcgcgt tttgataagc agttacttaa
    29341 tttaagtgac gatcgctaaa aacgactgtc actgtcctaa tcttatacga catccgaatg
    29401 agattaattt atcgccatcg cggcgttatt tcattcggat ttcatgttca agcaacacct
    29461 ggttgtttca gtcaacggag tattacatgt ccagaaggct tcgcagaaca aaaatcgtta
    29521 ccacgttagg cccagcaaca gatcgcgata ataatcttga aaaagttatc gcggcgggtg
    29581 ccaacgttgt acgtatgaac ttttctcacg gctcgcctga agatcacaaa atgcgcgcgg
    29641 ataaagttcg tgagattgcc gcaaaactgg ggcgtcatgt ggctattctg ggtgacctcc
    29701 aggggcccaa aatccgtgta tccaccttta aagaaggcaa agttttcctc aatattgggg
    29761 ataaattcct gctcgacgcc aacctgggta aaggtgaagg cgacaaagaa aaagtcggta
    29821 tcgactacaa aggcctgcct gctgacgtcg tgcctggtga catcctgctg ctggacgatg
    29881 gtcgcgtcca gttaaaagta ctggaagttc agggcatgaa agtgttcacc gaagtcaccg
    29941 tcggtggtcc cctctccaac aataaaggta tcaacaaact tggcggcggt ttgtcggctg
    30001 aagcgctgac cgaaaaagac aaagcagaca ttaagactgc ggcgttgatt ggcgtagatt
    30061 acctggctgt ctccttccca cgctgtggcg aagatctgaa ctatgcccgt cggctggcac
    30121 gcgatgcagg atgtgatgcg aaaattgttg ccaaggttga acgtgcggaa gccgtttgca
    30181 gccaggatgc aatggatgac atcatcctcg cctctgacgt ggtaatggtt gcacgtggcg
    30241 acctcggtgt ggaaattggc gacccggaac tggtcggcat tcagaaagcg ttaatccgtc
    30301 gtgcgcgtca gctaaaccga gcggtaatca cggcgaccca gatgatggag tcaatgatta
    30361 ctaacccgat gccgacgcgt gcagaagtca tggacgtagc aaacgccgtt ctggatggta
    30421 ctgacgctgt gatgctgtct gcagaaactg ccgctgggca gtatccgtca gaaactgttg
    30481 cagccatggc gcgcgtttgc ctgggtgcgg aaaaaatccc gagcatcaac gtttctaaac
    30541 accgtctgga cgttcagttc gacaatgtgg aagaagctat tgccatgtca gcaatgtacg
    30601 cagctaacca cctgaaaggc gttacggcga tcattaccat gaccgaatcg ggtcgtaccg
    30661 cgctgatgac ctcccgtatc agctctggtc taccaatttt cgccatgtcg cgccatgaac
    30721 gtacgctgaa cctgactgct ctctatcgtg gcgttacgcc ggtgcacttt gatagcgcta
    30781 atgacggcgt agcagctgcc agcgaagcgg ttaatctgct gcgcgataaa ggttacttga
    30841 tgtctggtga cctggtgatt gtcacccagg gcgacgtgat gagtaccgtg ggttctacta
    30901 ataccacgcg tattttaacg gtagagtaag tacgttgccg gatgcggcga aaacgccaca
    30961 tccggcctac agttcaatga tagttcaaca gatttcgaat attctgaagc aaacttgaac
    31021 ttatcatcag gcgaaggcct ctcctcgcga gaggcttttt tatttgatgg gataaagatc
    31081 tttgcgctta tacggctgga tttcgcccgg tttgcgagtt ttcagcaatt ttagtatcca
    31141 ggtgtattgt tctggtcgcg gaccaacaaa aatctcgact tcttcattca tccgccgcgc
    31201 aatcgtatga tcatccgcct ctaacagatc atccatcggt gggcgcacct gaatcgtcag
    31261 acggtgcgtc ttgccatcat aaatcggaaa cagcggaaca acgcgcgcac ggcacacttt
    31321 catcaaacga ccaatcgcgg gcaacgtcgc tttataggtg gcaaagaaat caacaaattc
    31381 gctgtgctca gggccatgat cctgatcggg taaataatat ccccagtagc cctgacgtac
    31441 cgactggatg aatggtttaa taccatcatt tctcgcatgc agacgaccgc caaaacgacg
    31501 acgcaccgtg ttccagacat aatcaaaaac cgggttgccc tgattatgga acatcgctgc
    31561 cattttctgc ccttgcgagg ccatcagcat ggcaggaata tcgacggccc aaccgtgcgg
    31621 caccagaaag ataactttct cgttattacg ccgcatctct tcaatgatct ccagcccttg
    31681 ccagtcaacg cgcggctgaa ttttctccgg cccgcgtatt gccaactcag ccatcattgc
    31741 catcgcttgc ggcgcggtgg caaacatctc atcaacaatc gcttcgcgtt cagcttcact
    31801 acgttctgga aagcagagcg acagattgat taacgcacga cggcgtgagc tttttcccag
    31861 tcgtccggca aaacgtccca gccgtgccag aatgggatca cggaactttg gcggcgttaa
    31921 agcgataccc gccatcgctg ctacgcccag ccatgctccc cagtagcgcg ggtggcgaaa
    31981 ggatttatca aactcaggaa tgtattcgct attatttttt ttcgtttcca tgcttttcca
    32041 gtttcggata aggcaaaaat caatctggtg atagtgtagc ggcgcaactt gccccgcacc
    32101 aaataaaaaa agccggtact gactgcgtac cggctgcgaa tggatgttaa ttaatcaaac
    32161 cgtagctgcg gcacaatctc tttggcctgc gccaggaatt cgcgacgatc ggagccggtc
    32221 agcccttcgg tacgcggcag ttttgccgtc agcgggttta cggcctgctg gtttatccat
    32281 acttcatagt gcagatgtgg cccggttgaa cgtccggtat taccggaaag cgcgatacgg
    32341 tcgccacgtt tcaccttctg tcccggtttc accagaatct tgcgcaagtg catataacgc
    32401 gtggtgtagc tgcgaccatg acgaatagcc acataataac ctgctgcgcc actgcgtttg
    32461 gcaaccacca cttcaccgtc acccactgaa agcactggcg taccctgcgg catggcgaaa
    32521 tcaacacctc tgtgtggtgc aacgcgaccg gtcacaggat tagtacgacg cgggttaaag
    32581 ttagacgaga tacggaactg tttcgccgtc gggaatcgca agaatccttt cgccagacca
    32641 gtaccgttac gatcgtagaa tttgccatct tcagcgcgga tagcgtaata atctttacct
    32701 tctgaacgca aacgtacgcc cagcagctgg ctttgctcac gtttaccatc aagcatttct
    32761 cgagacatta ataccgcaaa ttcatcgcct tttttcagtt tgcggaaatc catttgccac
    32821 tgcatggctt taatcactgc gctcacttcg gcgctggtta aaccggcgtt tctggcgctg
    32881 gcaacaaagc tccccccgac ggtacctttc agcagattgt taacccactc tccttgctgc
    32941 atttcgctgg tcattttaaa accgttagcg gcagtacggt cataggttcg ggtttcacga
    33001 cgagacactt cccaggtgag gcgctgcagt tcgccgtccg cggttaatgt ccaggagagt
    33061 tgttgaccga ttttcaggtt acgcaattct ttgtcggcag cagccagttg ggtgatatca
    33121 cccatatcaa taccatactg attgagaatg ctgcttagcg tatcgccagt ggaaacaaca
    33181 tattcatgca cgcccgcttc accggcgatt ttgtcatcca gttcgtcctg gggaatggct
    33241 tcatcttctt gtgcagcttg atcaatcggc tcactggctt caggtaagag cgaacgaatt
    33301 tcgttctgtt ccagctcaat ggttttgaca attggcgtgg catcgcggtg ataaacatag
    33361 ggccgccaga cagcgacggc cagagtaaga acggtgagcg accccaacat aacgcggtgt
    33421 ggtcgcggta aattattaaa cgccagggcg acagagcggg ctatctgttg cacgtaatca
    33481 cttcctcatt aatctccttt caggcagctc gcatactggt tggctaattg actcaggaat
    33541 tctgaatagc ttgttttacc cagtttgata ttcgtcccca ggggatccaa cgttcccata
    33601 cgaacggatg tccctcgtgc gacgctctca acgaccgctg gcctgaactg tggctcagca
    33661 aaaacgcagg ttgctttttg ctcaaccaac tgtgttctta tttcatgtaa acgctgcgcg
    33721 ccaggttgaa tctcagggtt aacggtaaaa tgaccaagcg gtgtcagtcc aaactgtttt
    33781 tcgaaatagc cgtaagcatc gtgaaaaacg aaataacctt tccccttgag cggcgcgagc
    33841 tcgttaccaa cctgcgtttc ggttgaggct aattgtgcct caaaatcctt caggttggcg
    33901 tcaagtttgg ctcgactttg cggcataagt tccactaatt ttccatggat tgcaaccgct
    33961 gtagcccgcg ctatctctgg ggaaagccaa agatgcatgt tgaaatcgcc gtgatggtga
    34021 tcttcgtcac ttttttccgc gtggtcgtga tcatcatcat cgccgtgaat acttttcatc
    34081 agtagcggtt tcacatcttc aagctgcgca atcgttacct gcttcgcttc tggtaatttg
    34141 cttaccggtt tttgcataaa cgcttccatc tccgggccaa cccaaacgac taagtccgcg
    34201 ttctgtaagc gttttacatc cgatggacgc agtgaataat catgttctga agcgccgtca
    34261 ggcagtaaaa cctccgtttc tgttacacca tcagcaatgg cagaagcgat gaacccaacg
    34321 ggtttaagcg aagcgacaac ggcggcatct gcggcctgtg ttgcacctcc ccagagagcg
    34381 gcggataatg ctgcgaaaag aagcgttttt ttatgtaaca taatgcgacc aatcatcgta
    34441 atgaatatga gaagtgtgat attataacat ttcatgacta ctgcaagact aaaattaaca
    34501 tgacaagtct ggtttccctg gaaaatgtct cggtttcttt tggccaacgc cgcgtcctct
    34561 ctgatgtgtc gctggaactt aaacctggaa aaattttgac tttacttggg ccaaatggcg
    34621 caggtaagtc gacactggta cgggtagtgc tcgggctggt aacacccgat gaaggggtta
    34681 tcaagcgcaa cggaaaactg cgcatcggct atgtaccgca gaagctgtat ctcgacacca
    34741 cgttgccact gaccgtaaac cgttttttac gcttacgccc tggtacacat aaagaagata
    34801 ttttgcctgc actgaaacgt gtccaggccg ggcacctgat taacgcaccg atgcaaaagc
    34861 tctctggtgg cgaaacgcag cgtgtactat tagcgcgagc attgttaaat cgcccgcaat
    34921 tattagtgct ggatgaaccc actcaaggcg tggatgtaaa tggccaggtg gcgttatatg
    34981 accttattga ccaactgcgt cgcgaactgg attgtggcgt tttaatggtc tctcacgatc
    35041 tgcatctggt aatggcaaaa accgatgaag tgctgtgtct gaatcaccac atttgttgtt
    35101 ccggcacacc ggaagttgtt tccctacatc cggagtttat ttctatgttt ggtcctcgtg
    35161 gtgctgaaca actgggtatc tatcgccatc atcataatca tcgtcacgat ttacagggac
    35221 gaattgtttt gcgtcgggga aatgatcgct catgattgaa ttattatttc ccggttggtt
    35281 agccgggatc atgctcgcct gtgccgcggg tccgctgggt tcgtttgtag tctggcgtcg
    35341 tatgtcttat ttcggtgata cgctggctca tgcctcatta cttggcgtcg cgtttggttt
    35401 gttgctggac gtgaatccat tctatgcggt aattgccgtt acgctgctcc tggcgggcgg
    35461 tctggtatgg ctggagaagc gtccacagct ggcgattgac acgttattag ggattatggc
    35521 gcacagtgcc ctgtcgctgg gcctggtggt cgttagtctg atgtctaaca ttcgtgttga
    35581 tttgatggct tacctgttcg gtgatttgct ggcagtgacg ccagaagatc tcatcgctat
    35641 tgcgattggc gtggtcatcg tggtggctat tttgttctgg caatggcgca atttgctgtc
    35701 gatgacgatt agcccggatc tggcgtttgt tgatggtgtg aaattacagc gcgtgaaatt
    35761 gttgttgatg ctggtgacgg cattgacgat tggtgtagcg atgaaatttg ttggcgcgtt
    35821 gattattact tcactgctga ttattcctgc agctactgcg cgtcgctttg cccgtacgcc
    35881 ggaacagatg gctggtgtcg ctgttttggt ggggatggtg gcagtaactg gcggtttaac
    35941 cttttccgcg ttttacgata cgccggcggg tccgtcggtg gtgctatgtg cggcactgtt
    36001 atttattctc agtatgatga aaaagcaggc cagctaatct atcgctgaac acatttgtcg
    36061 gatgcggcgc gagcgcctta tccgacctac ggttcgctat ctctggctgg cctgataaga
    36121 cgcgaacagt gtcgcatcag acacactgcc agtgtcggat gcggcgcgag cgaccaatcc
    36181 gacttacggc atttctggcg gcgttatgcc aaagtgattc cacgcccgcg tcgtcgccat
    36241 acgcccacgc ggtgtacgct gcaaaaagcc ttgctgaatc aaataaggtt ccagcacatc
    36301 ctcaatggtt tcacgttctt cgccaatggc tgccgcaagg ttatccagac ctaccggccc
    36361 accaaagaac ttatcgatta ccgccagcaa taacttgcgg tccatataat cgaaaccttc
    36421 agcatcgaca ttcaacatat ccaacgcctg agcagcgata tctgccgaga tggtgccatc
    36481 gtgcttcact tcggcgaaat cacgcactcg acgcagcaga cggttggcaa tgcgcggcgt
    36541 accgcgagct cgacgagcaa cttccagcgc gccgtcatca ctcatctcaa gccccataaa
    36601 gcgtgcgctg cgactgacga tatattgcag atccggcacc tgataaaact ccagacgctg
    36661 cacaatacca aaacgatcgc gcaacggtga tgtcagcgaa cctgcgcgcg tggttgcacc
    36721 aatcagggta aacggcggca aatcaatttt aatggagcgt gccgccggac cttcaccaat
    36781 catgatatcc agttggtagt cttccattgc cggatacaac acctcttcca ccactggcga
    36841 aagacggtgg atctcatcaa taaacaacac gtcatgcggt tcaaggttag tgagcatcgc
    36901 tgccagatcg cccgcctttt ccagcaccgg accagaagtc gtgcgtaaat taacgcccat
    36961 ttcattggcg acgatgttgg caagcgtagt tttacccaac cccggaggac cgaaaatcaa
    37021 cagatgatcg agggcatcgc cgcgcagttt cgctgctttg atgaaaatct ccatctgtga
    37081 acgaacctgc ggctgaccaa catactcttc cagtaattta gggcgaatgg cgcgatctgc
    37141 cacatcttcc ggcaaagtgg taccggcaga aatcagacgg tctgcttcaa tcatccttta
    37201 cctcataacg cggcgcgtag ggcttcgcga attaatgttt cactgctggc gtcagggcga
    37261 gcgattttgc tcaccatgcg gcttgcctct tgtggtttat agcccagcgc caccagcgcg
    37321 gcaactgctt cttgttcagc atcgtcggtc gccgggctgg caggtgatgt gagtaccagg
    37381 tcggcggctg gcgtaaagag atcgccatgc aaacctttaa atcggtcttt catttcaaca
    37441 atcaagcgtt cggcggtttt tttgccaata cccggcagtt tcaccagcgc ccccacttct
    37501 tcacgctcaa cggcattaac gaactgctgc gctgacattc cggagaggat cgccagcgcc
    37561 aacttcgggc cgacgccgtt ggttttgatc aactctttga acaatgtgcg ctcttgttta
    37621 ttgttaaaac cgtacagcag ttgcgcgtct tcacgcacca caaagtgggt gaaaacgatc
    37681 gcttcctgac ccgcttcagg gagttcataa aaacaggtca tcggcatatg cacttcatag
    37741 cctacgccgc ccacttcaat taacaccagc gggggttgtt tttcaatgat gatgcctctg
    37801 agtctgccta tcacatgacg ctcctgcgta atgaatcaaa gataatgctg tatgataaaa
    37861 aaatgctgga tagatatcca gcgaaggatg aagaaaactt gcgaggtgtc c
//
LOCUS       DAPHDX010000033        37619 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPHDX010000033 DAPHDX010000000
VERSION     DAPHDX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.033"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..316
                     /locus_tag="RH058_001615"
     CDS             155..316
                     /locus_tag="RH058_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137218.1"
                     /translation="MEFSFSPKLFVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(400..1635)
                     /locus_tag="RH058_001616"
     CDS             complement(400..1635)
                     /locus_tag="RH058_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV3137219.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1782..1858)
                     /locus_tag="RH058_001617"
     tRNA            complement(1782..1858)
                     /locus_tag="RH058_001617"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1822..1824),aa:Pro,seq:tgg)
     gene            complement(1901..1987)
                     /locus_tag="RH058_001618"
     tRNA            complement(1901..1987)
                     /locus_tag="RH058_001618"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1951..1953),aa:Leu,seq:cag)
     gene            complement(2008..2083)
                     /locus_tag="RH058_001619"
     tRNA            complement(2008..2083)
                     /locus_tag="RH058_001619"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2048..2050),aa:His,seq:gtg)
     gene            complement(2142..2218)
                     /locus_tag="RH058_001620"
     tRNA            complement(2142..2218)
                     /locus_tag="RH058_001620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2182..2184),aa:Arg,seq:ccg)
     gene            complement(2321..3706)
                     /locus_tag="RH058_001621"
     CDS             complement(2321..3706)
                     /locus_tag="RH058_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3137220.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3897..4637)
                     /gene="rffM"
                     /locus_tag="RH058_001622"
     CDS             complement(3897..4637)
                     /gene="rffM"
                     /locus_tag="RH058_001622"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV3137221.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4640..5992)
                     /gene="wzyE"
                     /locus_tag="RH058_001623"
     CDS             complement(4640..5992)
                     /gene="wzyE"
                     /locus_tag="RH058_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV3137222.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5989..7068)
                     /gene="rffT"
                     /locus_tag="RH058_001624"
                     /gene_synonym="wecF"
     CDS             complement(5989..7068)
                     /gene="rffT"
                     /locus_tag="RH058_001624"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV3137223.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7065..8315)
                     /gene="wzxE"
                     /locus_tag="RH058_001625"
     CDS             complement(7065..8315)
                     /gene="wzxE"
                     /locus_tag="RH058_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV3137224.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8317..9447)
                     /gene="rffA"
                     /locus_tag="RH058_001626"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8317..9447)
                     /gene="rffA"
                     /locus_tag="RH058_001626"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV3137225.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVIST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9452..10126)
                     /gene="rffC"
                     /locus_tag="RH058_001627"
                     /gene_synonym="wecD"
     CDS             complement(9452..10126)
                     /gene="rffC"
                     /locus_tag="RH058_001627"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV3137226.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10104..10985)
                     /gene="rfbA"
                     /locus_tag="RH058_001628"
     CDS             complement(10104..10985)
                     /gene="rfbA"
                     /locus_tag="RH058_001628"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV3137227.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11004..12071)
                     /gene="rffG"
                     /locus_tag="RH058_001629"
     CDS             complement(11004..12071)
                     /gene="rffG"
                     /locus_tag="RH058_001629"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV3137228.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12071..13333)
                     /gene="wecC"
                     /locus_tag="RH058_001630"
     CDS             complement(12071..13333)
                     /gene="wecC"
                     /locus_tag="RH058_001630"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV3137229.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13330..14460)
                     /gene="wecB"
                     /locus_tag="RH058_001631"
     CDS             complement(13330..14460)
                     /gene="wecB"
                     /locus_tag="RH058_001631"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV3137230.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14516..15562)
                     /gene="wzzE"
                     /locus_tag="RH058_001632"
     CDS             complement(14516..15562)
                     /gene="wzzE"
                     /locus_tag="RH058_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV3137231.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15574..16677)
                     /gene="wecA"
                     /locus_tag="RH058_001633"
     CDS             complement(15574..16677)
                     /gene="wecA"
                     /locus_tag="RH058_001633"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV3137232.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIIDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16917..18176)
                     /gene="rho"
                     /locus_tag="RH058_001634"
     CDS             complement(16917..18176)
                     /gene="rho"
                     /locus_tag="RH058_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV3137233.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18261..18362)
                     /gene="rhoL"
                     /locus_tag="RH058_001635"
     CDS             complement(18261..18362)
                     /gene="rhoL"
                     /locus_tag="RH058_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV3137234.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18503..18832)
                     /gene="trxA"
                     /locus_tag="RH058_001636"
     CDS             complement(18503..18832)
                     /gene="trxA"
                     /locus_tag="RH058_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV3137235.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18963..20228
                     /gene="rhlB"
                     /locus_tag="RH058_001637"
     CDS             18963..20228
                     /gene="rhlB"
                     /locus_tag="RH058_001637"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV3137236.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20362..21846
                     /gene="gppA"
                     /locus_tag="RH058_001638"
     CDS             20362..21846
                     /gene="gppA"
                     /locus_tag="RH058_001638"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV3137237.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21893..23914)
                     /gene="rep"
                     /locus_tag="RH058_001639"
     CDS             complement(21893..23914)
                     /gene="rep"
                     /locus_tag="RH058_001639"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV3137238.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24131..24580
                     /locus_tag="RH058_001640"
     CDS             24131..24580
                     /locus_tag="RH058_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV3137239.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQISTAYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24733..25014
                     /gene="ppiC"
                     /locus_tag="RH058_001641"
     CDS             24733..25014
                     /gene="ppiC"
                     /locus_tag="RH058_001641"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV3137240.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25060..26535)
                     /gene="ilvC"
                     /locus_tag="RH058_001642"
     CDS             complement(25060..26535)
                     /gene="ilvC"
                     /locus_tag="RH058_001642"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV3137241.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26685..27578
                     /gene="ilvY"
                     /locus_tag="RH058_001643"
     CDS             26685..27578
                     /gene="ilvY"
                     /locus_tag="RH058_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV3137242.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27630..29174)
                     /gene="ilvA"
                     /locus_tag="RH058_001644"
     CDS             complement(27630..29174)
                     /gene="ilvA"
                     /locus_tag="RH058_001644"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV3137243.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29177..31027)
                     /gene="ilvD"
                     /locus_tag="RH058_001645"
     CDS             complement(29177..31027)
                     /gene="ilvD"
                     /locus_tag="RH058_001645"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV3137244.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVANSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIEFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31092..32021)
                     /gene="ilvE"
                     /locus_tag="RH058_001646"
     CDS             complement(31092..32021)
                     /gene="ilvE"
                     /locus_tag="RH058_001646"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV3137245.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32041..32304)
                     /gene="ilvM"
                     /locus_tag="RH058_001647"
     CDS             complement(32041..32304)
                     /gene="ilvM"
                     /locus_tag="RH058_001647"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV3137246.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32301..33947)
                     /locus_tag="RH058_001648"
     CDS             complement(32301..33947)
                     /locus_tag="RH058_001648"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV3137247.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDEFENVWPLVPPGASNSEMLEKLS"
     gene            complement(33950..34000)
                     /locus_tag="RH058_001649"
     CDS             complement(33950..34000)
                     /locus_tag="RH058_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137248.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34087..34185)
                     /gene="ilvL"
                     /locus_tag="RH058_001650"
     CDS             complement(34087..34185)
                     /gene="ilvL"
                     /locus_tag="RH058_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV3137249.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34538..36058
                     /locus_tag="RH058_001651"
     CDS             34538..36058
                     /locus_tag="RH058_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV3137250.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     RAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36083..36421)
                     /gene="maoP"
                     /locus_tag="RH058_001652"
     CDS             complement(36083..36421)
                     /gene="maoP"
                     /locus_tag="RH058_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV3137251.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36540..37379
                     /gene="hdfR"
                     /locus_tag="RH058_001653"
     CDS             36540..37379
                     /gene="hdfR"
                     /locus_tag="RH058_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV3137252.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37475..37550)
                     /locus_tag="RH058_001654"
     tRNA            complement(37475..37550)
                     /locus_tag="RH058_001654"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37515..37517),aa:Trp,seq:cca)
BASE COUNT         9186 a        10243 c         9478 g         8712 t
ORIGIN      
        1 acgctctcct gattttccaa ttcattttct ggataaataa ataatttatt tttgtcacta
       61 ttatttatgt aatcatcctg tcaaggagag ggatctcaat tatcaatgct taattacgtc
      121 atcattttga tgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt cacccaaact
      181 ttttgttgtt gctgtcgccg ccgctcttcc tctcatggcc agcgcagcag ataccccgtc
      241 aactgccacc gcacgcaaag gctttgccgg atacgatcac ccaaaccagt atccacagaa
      301 cgctgcaatt tattgaattt gcacgatcat gtaggccgga taaggcgttt acgccgcatc
      361 cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt tacttattca ccaccagcaa
      421 atgtgcctgc ataatgtcac tggccgggcg accgtgcgcc agcaaatcag ccattgcttt
      481 aagatatggc ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagccc
      541 cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg cagcctcccc agcacgcttt
      601 taacacgtta caactgcgac actgcgccgg taattgctta aatttatctt caccaaacac
      661 ctgctgttgc ggggaatcga tcatttctgc aattgtttgc tgatgcatat tccccagccg
      721 atattgcgga taaacatagt gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac
      781 cgagcgccca caggttggct gatgatggca aaccgcaccc ggcgcaccga caaaattggc
      841 aaacgcccat tcgatattca tcacgaaaat cttgccgacg tcgcgtttga tccagtggtc
      901 gaatatcgcc accagaaatt caccaaattc atcggggcgc accgaccatt ccgtcagttc
      961 cccctgaata tcaccaggcg catgtagttt cagtccatcg ctggcagctg tttcatcagc
     1021 caggcgctcg accaccggaa taaactggat gaattcgact cccgcatcgc acaaaaaatc
     1081 ataaacctgc aacggttgct gcgcgctggt gcggttgacg cagaccagca cgttatagtc
     1141 gacatgatgt ttttgcagca gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc
     1201 acctttggtc acgcgatatt gattgtggat ctcagccgga ccgtccagcg ataacccaac
     1261 aagaaaatga ttttctgcca gaaatgcaca ccatttatca tcgagcagca cgccgttagt
     1321 ctggaagctg ttacttatct tcctgccagc accgtatttc gcctgtaact ttacggcacg
     1381 gcggtaaaaa tccagcccga gtaacgttgg ttcgccgccc tgccaggtaa aagccacttc
     1441 gttttgcggt tcgctggcag cgatatagtg acggacatac gcttccagcg tgtcatcgtc
     1501 catatgcgtg actggctttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc
     1561 acagttcaga ttacaatcgg aaccactcgg tttcgccatc acatgaaaag cacgcgttgg
     1621 aacctgttgc agcatggtca ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa
     1681 atcataccgt ggggcaatga gttattacgg gcgtttattg cgaagggagg gtgacgaaat
     1741 gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt
     1801 cgaacctccg acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa
     1861 atactgcttt ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt
     1921 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
     1981 ccttcgcatt gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa
     2041 ctggaatcac aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta
     2101 cgcggtatta ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt
     2161 cgaacctgag acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt
     2221 agcgccgttg cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa
     2281 ataaattgcg cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg
     2341 ctttcccgtg gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga
     2401 tgatgccaac aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc
     2461 aaatcaggaa tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat
     2521 ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa
     2581 accatggcac catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg
     2641 gattgggaat gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca
     2701 cacccgcaac gggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt
     2761 ttttcgccag tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga
     2821 gcgcagccgt cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg
     2881 caaaagtcag tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga
     2941 cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg ccacagcacc ttgcctacgg
     3001 cactgcgcag cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca
     3061 cgccctggta ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg
     3121 caaagaaacc gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa
     3181 agccaaagaa aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga
     3241 tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg
     3301 ccaccagcgc cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact
     3361 ggacataaac gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccaat
     3421 aagaccaggc ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga
     3481 acgaaccggt aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga
     3541 cgaatagccc ggcgatgata taggccaaca acacggatgg cccggcccat ttcaaggtac
     3601 tggcggcccc cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat
     3661 gtcgagcttc cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg
     3721 tgtttgcatg ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg
     3781 ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat
     3841 aaaaaagcag acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag
     3901 aggttgccgg tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg
     3961 ctcggctgcg aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt
     4021 gcgcgtttta cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca
     4081 tctggatgca ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca
     4141 acggtgacga tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg
     4201 ggtttaaaat aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta
     4261 gcttcagttt gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct
     4321 ttgcctgcgc gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc
     4381 gcctgcgggt actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat
     4441 tcggcagcgt taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg
     4501 gcattaatgg caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg
     4561 gcgtgctgca tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc
     4621 gtggtgttgt tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt
     4681 tatgaatgag tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg
     4741 cgccgaagac cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg
     4801 ccagcacgat catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat
     4861 agcgattagt ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc
     4921 caaccacaat cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta
     4981 taagcgtagg cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt
     5041 tggctgagtt cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat
     5101 agaaatcgcg gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca
     5161 gcaacgccag attctcccat ggcgagaagg tgtcgcgagt gagatagaga aacgtataga
     5221 acgcttcatc gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca
     5281 caatccccag cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc
     5341 caataaacag gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca
     5401 tataagtcag caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat
     5461 cctggcgcag aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta
     5521 acgccacgcc ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca
     5581 aaaagccgtt gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca
     5641 cccacgtaag attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag
     5701 caacgcgttt gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg
     5761 cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata
     5821 ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga
     5881 agacattgaa gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca
     5941 gcgtgcagag cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc
     6001 cccggcggca atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc
     6061 aatggtgttt ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt
     6121 gagatcgtcg gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg
     6181 attttcccgg ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat
     6241 gccctgctgg cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata
     6301 ggcgtcaaat tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag
     6361 ccccgcctga cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac
     6421 caccactttt accgtatcgc caaattgctg atgaacggcg cacaaggcag caatatggtc
     6481 attgctgcgg tcaccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc
     6541 gttcgccatc gtattgagag aagggtccat ccgcgtcggg aagtacagca gttcgccccg
     6601 cacctttgga tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc
     6661 gactcgcttt tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga
     6721 aagctcatac aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc
     6781 actcagcaga gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg
     6841 acggttagct tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa
     6901 ttgcacagaa agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa
     6961 ctcgcgcgcg tgctcgctcg tcgcagccag cgcgtcattg aaaaaccgca aaacggttcg
     7021 gttatggtga gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc
     7081 cagagtaaaa acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc
     7141 tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa
     7201 gtgaactggc tgatttccgc cagaatataa aaccgcaatg acgctttggc gatcaccaga
     7261 taaccaaaga cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga
     7321 tcgcgcatag cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc
     7381 cagacggtaa aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact
     7441 tcccgggtga tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg
     7501 aacgatgccg taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc
     7561 cccacttcat cccagctata ctgcgccgcg agcagtttac gcatcatgat gtaagcaaca
     7621 ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga
     7681 ccgttatccc agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg
     7741 atggcggcag gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca
     7801 taaccgccca aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc
     7861 agcgataacg cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg
     7921 ccccaggcga tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta
     7981 ttaccaaaca atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc
     8041 gtagagaaac caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga
     8101 ttatcatggt actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc
     8161 acgccgagca cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca
     8221 aatgacacag ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg
     8281 gccgccgtcc acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt
     8341 cgcaattacc gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag
     8401 gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc
     8461 cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag
     8521 aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc
     8581 gttctgcacg cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg
     8641 cgctaacgca tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc
     8701 tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc
     8761 aatatcgcgc caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc
     8821 cttttcacgg atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc
     8881 gccttcacca cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc
     8941 aatggttccc agtgcacgcc ctttgtaagt ggatatcacg ccctgagcgg catcttctac
     9001 cacaaacaga ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc
     9061 cgcgtaatgg accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt
     9121 ttcgtcgatg ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag
     9181 cacaaaggca ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg
     9241 ctggatatcg agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa
     9301 cactttggcg ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc
     9361 atcgccacac agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac
     9421 caccggcggt gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt
     9481 ctacatttgc accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg
     9541 ccacccgcaa agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct
     9601 cagcacctgc accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc
     9661 gtaaagagac atagccgcga atatcgccgg acgccgcacg taaaatcaga cattgatgat
     9721 caaaggtgcc gcgcacggca ttttcaatcc actgtgcata aaagcgtctg ctggcgtcag
     9781 gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta
     9841 atgcgggaat atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg
     9901 gtagtgccaa atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca
     9961 gttcacccgt atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt
    10021 ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga
    10081 aggcgttttc ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc
    10141 agatattggc cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca
    10201 tcgagccagc cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt
    10261 ttttctactg tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc
    10321 agccaggcaa atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc
    10381 tggttgatgg aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac
    10441 tccacgactt tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt
    10501 tttggctttt cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag
    10561 cgttccgggt ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca
    10621 tgacgcagct tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa
    10681 gaaggttcac cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg
    10741 ctgggctgtt cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc
    10801 tggaaataac ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac
    10861 atcagcaccg acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc
    10921 gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct
    10981 ttcatgtttg cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg
    11041 ccgtcctgca cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc
    11101 attccacttt caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca
    11161 tcaatggcat aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga
    11221 tagtgcgcca cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt
    11281 tccacaacat cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact
    11341 ttcccggtgg tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca
    11401 cggatttgct gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc
    11461 atcagcggga tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg
    11521 atcagcgtcg gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctactggct
    11581 tttgacgcgg aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc
    11641 gtcgagtgca ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat
    11701 tttttatctt ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc
    11761 ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt
    11821 tcggctgcca gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct
    11881 gcccgatcgc agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc
    11941 agcgacatca ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt
    12001 tcgttgatga tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga
    12061 atttttctca tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt
    12121 cgccattgat aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg
    12181 ccagcgcctc gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt
    12241 ggatgttagg ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga
    12301 tttccatcgc cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc
    12361 aggcgatttt cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg
    12421 ctttcacctg atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga
    12481 taagccgcgc ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt
    12541 gaccgcccac gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc
    12601 gaatcagttc ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa
    12661 aagcgatatt cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg
    12721 agttagtgac gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg
    12781 aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct
    12841 ggcccggtaa cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct
    12901 gctgtgggaa agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg
    12961 accccaccgg cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa
    13021 tggagcgagc agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg
    13081 tgggtacagc aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac
    13141 cgccttctac ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc
    13201 cacgattgat ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc
    13261 gtgaggcaaa cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg
    13321 tcgcaaaact catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc
    13381 ctgaccatca ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt
    13441 taaaagacgc gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt
    13501 acccgccgtc accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc
    13561 cagcgaaggc gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt
    13621 catcagccag acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg
    13681 ccccagaatg cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat
    13741 ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc
    13801 acgaccgaaa ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat
    13861 aaacgggtaa tttgccgcta gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg
    13921 cacccataac agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac
    13981 gttttcacgc agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag
    14041 atgcccggtc aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg
    14101 taaaccagcc tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc
    14161 cagcgtcgtt gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat
    14221 aggttttagc ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat
    14281 tatgttgaga tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc
    14341 ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg
    14401 caccaacgga gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac
    14461 atcgattctc ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt
    14521 tcgagcaacg gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca
    14581 taatcatcag gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca
    14641 aatagcgata ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca
    14701 tggcccgatt ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca
    14761 gtcgagcctg gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg
    14821 caggcacatc tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct
    14881 caatgctgtt catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct
    14941 gagctttcat ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc
    15001 tggctgcacg ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg
    15061 cggtttcggc aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa
    15121 actggatgtt gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca
    15181 tccgctgctt gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca
    15241 gttgcataac aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca
    15301 tgtttgaacg ggcatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca
    15361 tattcaccgt tggacgatcg gtaatcgccg tcgcgctcca ctcttgtcga gcaaaaaaag
    15421 tatacgccag cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag
    15481 cccacaaggt acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt
    15541 tcccaggcat tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc
    15601 acgatttcta cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg
    15661 cttaatgcaa tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc
    15721 cgggacaaaa tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag
    15781 ggtaatcagc acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat
    15841 atgctgacgg tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc
    15901 caccatatcc attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg
    15961 ggttttgccc tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact
    16021 gcccgcatca cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat
    16081 gtatggcagg atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc
    16141 gaaccacaaa atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc
    16201 atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag
    16261 gaagtaacca aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact
    16321 gagataaagt ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg
    16381 gatttttacg ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc
    16441 acaagcgaga tagagagatg catgcggaat atagtaatcg ataattccga acgtgaagca
    16501 aatccctgcg taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg
    16561 gaagtttggt ttatccacta aaccgacttt ttttgccact ttacgggcaa aaaacagaaa
    16621 cagtgtcgtg aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat
    16681 gcatgctctc agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg
    16741 gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg
    16801 cataacatgc cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta
    16861 cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat
    16921 gagcgtttca tcatttcgaa gaaatcgtca ttggtcttag tcatggccag tttattaatg
    16981 aggaattcca ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt
    17041 ttctgcagtt cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag
    17101 tcgatagccg ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg
    17161 cctgtacctt taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc
    17221 gccgtcgcga taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag
    17281 cgtttcggac gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc
    17341 ggaacaacag tgttgtaagc gcgcgcaaga cgagtgatgg agtcgagcag aatgataacg
    17401 tctttcttgt gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg
    17461 tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc
    17521 atctcggtta cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg
    17581 tggttgtaag caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc
    17641 ggtgccacaa tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga
    17701 gcggttaaat cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc
    17761 agcggggtta agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta
    17821 acttcgttaa ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta
    17881 ccagagatgg tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag
    17941 atgtcatcag gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc
    18001 tgcaatatct ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc
    18061 aggatggcaa aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt
    18121 tcgccgagag tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg
    18181 gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga
    18241 tcatccatgc ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag
    18301 tacgcagaac tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc
    18361 atttaagata aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt
    18421 aatacgaagt caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg
    18481 ggcacccgaa catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac
    18541 ctttagacag tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag
    18601 tcgggatacc acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt
    18661 ttgcaacggt cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca
    18721 ttttgcacgg accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga
    18781 gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact
    18841 ccacaggaat aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg
    18901 ttgactttat ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca
    18961 ctatgagcaa aacacattta acagaacaga agttttccga cttcgccctg catccgaagg
    19021 ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc
    19081 ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa
    19141 cgatggcgtt tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc
    19201 gcaaggtgaa tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga
    19261 tccatgccga cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg
    19321 gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg
    19381 gcactacggg gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc
    19441 aggtggtggt actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc
    19501 gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca
    19561 cgctttcgta ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg
    19621 aagtggaacc ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta
    19681 acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga
    19741 ttattttcgc caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg
    19801 gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg
    19861 atgaatttac ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt
    19921 tgcatattcc ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt
    19981 acgttcaccg tattggtcgt acaggtcgcg caggcgcaag cggtcactct attagcctgg
    20041 cgtgtgaaga gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc
    20101 cggtaagcaa atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca
    20161 cgcgcccacg tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt
    20221 caggttaaat aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat
    20281 gagtcccgat gagcgtactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag
    20341 acagattgaa ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc
    20401 ggttcgaata gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg
    20461 acgcgaatta aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctctct
    20521 aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat
    20581 attcctccct cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg
    20641 ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt
    20701 gaagaggaag cacgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag
    20761 cgcctggtgg tggatatagg cggagccagt actgaactgg taaccggcac gggtgcacaa
    20821 accacctcgt tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc
    20881 gatcgtaatc tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta
    20941 cgtccggttg ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg cgcttccggc
    21001 accgtgcagg cgttacagga aatcatgatg gcacagggaa tggatgaacg cattaccctg
    21061 gaaaagttgc agcaactgaa acagcgcgcc attcattgcg gtcggctgga agagctggag
    21121 attgacgggc tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc
    21181 gccattttta ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa
    21241 ggcctggtct acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg
    21301 cgtaatattc agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt
    21361 gcagctaact tcttcgatca ggtggaaaat gaatggcatc ttgaagcaat aagccgcgat
    21421 ttgctcatca gcgcttgtca gcttcatgaa atcggcctga gcgttgactt caaacaagcg
    21481 ccgcaacacg ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag
    21541 aagaaactgc tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg
    21601 catcagcaaa atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actacgcctg
    21661 gctatcattt ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct
    21721 aaccatgaac tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt
    21781 aaagagatta ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg
    21841 cattaatgag taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc
    21901 ctcgttttgc cgccatcatc gctttcaaat tcgccagatg gctttgccct ttctgcatcc
    21961 gttcttcggc gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct
    22021 ccagcaaaaa gcggctcggc tccgggcgca ccagttcgcc atactgacgg cgttctttac
    22081 acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct
    22141 cctcatcgat attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac
    22201 cgaccatgta gacataagga aactccagcc ctttcgacgc gtggagagtc atcagttgca
    22261 cctgatccag ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg
    22321 tcaccacctg ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca
    22381 tccagctaaa cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg
    22441 atgtttcgta cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcagcaa
    22501 tcggctcccg ctccgccagg cgctggattt ctgccaacca gtgagtgaag cgggtcaatg
    22561 cttcataacc acgtccgcta agggtctgac tcaggcccat atcaaagctg gcggtaaaca
    22621 tgcttttatt gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct
    22681 ctcgcttcgg cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca
    22741 cacgcagata agccagcaaa tccttgattt caggacgaga gaaaaacgac gtaccaccag
    22801 aaattttgta cgggatgcgg ttttgcatca ggaatttttc aaatacccgc gactgatggt
    22861 tgccgcgata aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa
    22921 tcagttcacc ggtgacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta
    22981 gctctgtgcc ataacccagt tcggagaaca gacgcttttc aaagacgtgc ggattattgg
    23041 cgatcaggat gttcgccgct ttcaaaatac gtccggaaga gcggtagttt tgctcaagct
    23101 taatcacctt cagcgccgga aaatcctggc tcaacagcac caggttttgc ggacgtgcgc
    23161 cgcgccagga gtagatcgac tggtcgtcat cgccaactac ggtaaagcgc gcgcggctgc
    23221 ccaccagcag tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca
    23281 gcaggtagcg aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca
    23341 acgtcggcaa taaaatcaga tcatcgaagt cgagaacgtt acaggctttc aggtgtgcat
    23401 catacagccc ataacaatgg gcaaaaatac gatcccgctc gccctttgcc tctgccgccg
    23461 cctgcgccgg agttttgaga tcgttcttcc agttggagat ggtcgaaatt agctgttgca
    23521 ggagaacttt gtcatcttca atcagcccct cagtcagctc tttgagcaac gccagctgat
    23581 cggtatcgtc aaacaatgag aagttcgctt tcatcccaag cgccgcatac tcacgcttga
    23641 tgatatccag ccccagcgtg tggaaggtgg agatcatcag ccctcgcgcc tctttgcgac
    23701 ccagcgtctg cccgactcgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg
    23761 ccgcgatatg tcgtgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga
    23821 taacacgagt tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt
    23881 cgacagcttg ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg
    23941 ggggaggatt gtagcagaat cgcatttgaa tgtgaccgag tgaaaaccgc taaccggatg
    24001 gctgcggatg tttaaatcag agagggaagg ccgaaaccgg agttcatcat gaatccatta
    24061 gacttagaaa atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag
    24121 aggtttactc gtggcgatta atttcagccc caaggttggt gaaatactgg aatgcaactt
    24181 tggtaactat cctgttagcc agaatggaca gatcagtacg gcgtattacg atggccgcat
    24241 tccacctgag atgataaaaa ataggttggt tgtcgtgctc aacggaaaaa ttaatggcaa
    24301 cgcatgtatc gtcgttccat tatcaaccac tcgcgaccat gacaagttaa atcgtggaat
    24361 gcatgttgaa atagccagta atgttatcaa tgacctgcaa ttctttgatc agcagatacg
    24421 ttgggctaaa gccgacctgg tacaacaagt cagtcgaaac cgattaaaca gagcaaggac
    24481 ttatcggggg tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc
    24541 agtcatcaag tcaatcaatg ccatatcgct cataaattga caataaaatc accatccccg
    24601 ataatgactc tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc
    24661 ccggattttc cgggcttttg ccatatatgt ccccccctcc ctgactgaca atttttaatt
    24721 caaggcacga tcatggcaaa aacagcagca gcactgcata tccttgtaaa agaagagaaa
    24781 ctggctctgg aacttctcga gcagattaag aacggtgccg atttcggcaa gctggcgaag
    24841 aaacactcca tttgcccatc aggcaaacgc ggcggtgatt taggtgaatt ccgccagggt
    24901 cagatggttc cggcgttcga taaagttgtg ttctcttgtc cggtactgga gccaaccggc
    24961 ccgctgcaca cccagttcgg ttaccacatc attaaggtgc tgtaccgtaa ctaatagcaa
    25021 ggccttctcc gggagaaggc cttgaactct gaatcgctat taacccgcaa cagcaatacg
    25081 tttcatatcc gtcatatagc cgcgcagttt cttacctact tgctcaatcg catggctgcg
    25141 aatcgcttca tttacatcac gcagctgcgc attatctacc gcaccttccg gaatagcttt
    25201 acccaggtcg cccggttgca gttctgccat aaacggtttc agcagcggca cacaagcgta
    25261 agagaacaga tagttaccgt actcggcagt atcagatata accacgttca tttcatacag
    25321 acgcttacgg gcgatagtgt tggcaatcag cggcagttcg tgcagtgatt cataatatgc
    25381 agactcttca atgatgccgg aatcgaccat ggtttcgaac gccagttcaa cgcccgcttt
    25441 caccatcgcg atcatcagta cgcctttatc gaagtactcc tgctcgccga ttttgccttc
    25501 atactgcggc gcggtttcga aggcggtttt gccggtctct tcacgccagg tcagcagttt
    25561 cttatcatcg ttggcccagt ccgccatcat accggaggag aattcgccgg agatgatgtc
    25621 gtccatatgt ttctggaaca ggggtgccat gatctctttc agctgttcag acagcgcgta
    25681 agcacgcagt ttcgccgggt tagagagacg gtccatcatc agggtgatgc cgccctgctt
    25741 cagcgcttcg gtgatggttt cccaaccgaa ctgaattagt ttttctgcgt atgccggatc
    25801 ggtgccctct tccaccagct tgtcgaagca cagcagcgag ccagcctgca acataccgca
    25861 caggatggtt tgctcaccca tcaggtcaga tttcacttcc gcaacgaagg acgattccag
    25921 cacgcccgca cggtgaccac cggttgctgc cgcccatgct ttagcgatcg ccataccttc
    25981 gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc ggtacgccga atccacgttt
    26041 gtactcttca cgcacttcgg tgccagggca tttcggcgca accatgacta cggtgatatc
    26101 tttacggatc tgctcgccca cttcgacgat gttgaaaccg tgcgagtaac ccagcgccgc
    26161 gccgtctttc atcagtggct gtacggtgcg cactacatca gagtgctgct tgtccggcgt
    26221 caggttaacc accagatccg cctgcgggat cagttcttcg taagtaccaa ctttaaaacc
    26281 gttttcggtc gctttacgcc aggacgcgcg cttctcggca atcgcttctt tacgcagagc
    26341 gtaggagata tcgagaccag aatcacgcat gttcaggccc tggttcagcc cctgtgcgcc
    26401 acagccgacg ataaccactt ttttaccctg aaggtagctc gcgccgtcgg cgaattcgtc
    26461 gcgccccata aagcgacatt tgcccagctg tgccagctgc tggcgcaggt tcagtgtatt
    26521 gaagtagtta gccatggtga ttcctcgtga tgttgtgctt cttattgttc ggttcgcgtt
    26581 tgcgagattg aattcactat atgacaggaa atttattgcg gaaattgata tattcacaac
    26641 gtcacattgc aatttctgca acgtcaacat tgagggctgc gccattggat ttacgcgatc
    26701 tgaaaacctt cctgcatctg gcggaaagcc gccattttgg ccgcagcgcg cgggcgatgc
    26761 acgttagccc atccacgctc tcacggcaga ttcagcgcct ggaagaagat cttggtcagc
    26821 cgctatttgt gcgcgataac cgcacggtaa cgctgaccga agcgggcgaa gagttacgcg
    26881 ttttcgccca gcaaacattg ttgcagtatc agcaactgcg ccacaccatc gatcagcaag
    26941 ggccgtcgct ctctggcgag ttacatattt tctgctcggt gaccgccgct tacagccatc
    27001 taccgccgat tctcgaccgc ttccgcgcgg aacacccgtc ggtggagatc aaactcacca
    27061 ctggtgatgc ggcagatgca atggaaaaag tggtcacggg agaagcggat ctggcgattg
    27121 ccgggaaacc ggagacgcta cccggcgctg tggcgttttc gatgctggag aatctggcag
    27181 tagtgctgat tgcccccgcg ctgccctgcc cggtgcgtaa tcaggtatct gtcgaaaagc
    27241 ccgactggtc aacggtgccg tttattatgg ccgatcaggg gccggtacgc cgccgcattg
    27301 aactgtggtt tcgacgcaat aaaatcagca acccgatgat ttacgccacg gttggcgggc
    27361 atgaagcgat ggtatcgatg gtggcgctcg gctgtggtgt ggcgctgttg ccggaagtgg
    27421 tgctggaaaa cagcccggag cctgtacgca accgggtaat gattttagat cgcagcgatg
    27481 agaaaacgcc gtttgaactg ggcgtgtgtg cacaaaaaaa gcggctgcat gagccgctga
    27541 ttgaggcgtt ctggaggatt ttgccgaacc acaaatgacg ttgtcgcgcg ggtaggcctg
    27601 ataagcgaag cgctatcagg catttttccc taacccgcca aaaagaacct gaacgccgga
    27661 ttattggttt cgtcgtggca atcgtagccc agctcattta gccgggtttc gaaatccggt
    27721 tcatggtcgc cgagttcgaa cgccgccagt acgcgcccgt agtcggtgcc atggctgcga
    27781 tagtggaaca aagaaatgtt ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg
    27841 cccggtgatt ccgggaattc gaagctgtag aggcgttcct gcaacggatg tgacggacgc
    27901 ccgccgacca tatagcgcac atgcaacttc gccatttcgt cgtcggagag atccaccacg
    27961 ctgtagccgc cgtcgttgag catctgcaaa atttctttgc gctcttcgag gccgcggctc
    28021 aggcgcacgc cgacaaagat gcaggcgttt ttggcatcgg caaaacggta gttgaactcg
    28081 gtgaccgatc gcccgccaag cagttggcag aatttgagga agctgccttt ttcttccgga
    28141 atggtcaccg ccaacaacgc ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag
    28201 cgcaggccgt ggaagttcac gttggcaccg gaaagaatat gcgccagccg ttcgccgcga
    28261 atgttgtgct gggcgatata ttttttcatt cccgccagcg ccagcgcgcc agagggttcc
    28321 gccaccgcgc gcacatcttc gaataaatcc ttcatcgccg cacagatcgc atcactatcg
    28381 acggtgatga tgtcgtcgag atactcctgg cataaacgga aggtttcgtc accgatgcgt
    28441 tttaccgcta cgccttcagc aaatagccct acgcgcggca gatcaaccgg atgacccgca
    28501 tccagcgctg ctttcaggca ggcggagtct tccgcttcta cggcgatcac tttgatttgt
    28561 ggcatcagtt gtttgatcag caccgcaacg cccgccgcca gaccgccgcc gccgactggc
    28621 acaaatacgc ggtcgagatg ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc
    28681 ccggcaatca ccatcggatg gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac
    28741 agttcgatcg ctttggcttt cgcttcatca aagttcgcgc cgtggagcag cacttcgccg
    28801 ccgaagccgc gcaccgcatc aactttgata tcggcggtgg cggttggcat gacgatcagg
    28861 gccttcacgc ctaaccgtgc ggaagaaaac gcgacgccct gcgcgtggtt acccgcagaa
    28921 gcggtaatca cgccgtgcgc tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg
    28981 ccacgaagct taaagctgtg cactggctgg cgatcttcgc gcttcaccag aatcacgtta
    29041 tcgagacgcg acgacagttt ttccattttc tgtagcggcg tgacctgcgc cgcttcgtag
    29101 accggcgcgc gcagcaccgc tcttaaatat tcggcacctt ccggggtacc ggacaggggt
    29161 tgcgagtcag ccattattaa ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg
    29221 cgctggttgc caggctggcg taggcgcgca acgcaaagga gacctgacgt tcgcgatttt
    29281 ttggcgtcca ggctttgtcg ccacgagctt cctgcgcttc acgacgcgcc gccagttcgg
    29341 catcgcttac ctgtaactga atgccacggt tcggaatgtc gatagcaatc atgtcgccgt
    29401 cttcaatcag accaatgctg ccgccgcttg ccgcttccgg cgagacgtgg ccgatggaaa
    29461 gaccagaggt gccgccggag aagcgtccgt cggtgatcag cgcgcaggct ttgccgaggc
    29521 ccatcgattt caggaagctg gttgggtaga gcatttcctg catgccgggg ccgcctttcg
    29581 ggccttcgta acggatcacc accacatcgc cagcgacaac tttgccgccg agaatcgctt
    29641 ctaccgcgtc gtcctggctt tcgtacactt tcgccggacc ggtgaatttg aggatgctgt
    29701 catcgacgcc cgcggtttta acgatgcagc cgttttctgc gaaattaccg tagagcaccg
    29761 caaggccacc gtctttgctg taggcgtgtt ccagcgagcg gatgcagccg tttgcgcgat
    29821 cgtcatcgag agaatcccaa cggcaatcct gcgagaatgc ctgtgtggta cgaatgcccg
    29881 ccgggccagc gcggaacata ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt
    29941 gttccagcgt ttgcggcaac gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc
    30001 ccgcacgatc caactcgccg agaataccga taacaccacc agcacgatga acatcttcca
    30061 tatggtactt ctgcgtactc ggcgcgactt tacacagctg cggtaccttg cgggagagct
    30121 tatcgatatc actcatggtg aattcgattt ccgcttcctg cgccgccgcc agcaggtgaa
    30181 gtaccgtgtt ggtagagccg cccatcgcga tatccagcgt catggcattt tcaaacgccg
    30241 ccttactggc gatattacgc ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg
    30301 tcaattcaac aatgcgctta ccagcattga ggaacagttg cttgcgatcg gcgtgggtcg
    30361 ccagcagcga gccgttgccc ggctgcgaca ggcccagtgc ttcggtcagg caattcatag
    30421 agttagcggt aaacatcccg gagcaagatc cgcaggttgg acaggcggaa cgttcaacct
    30481 gatcgctctg ggagtcagaa actttcgggt ctgcgccctg gatcatcgca tcaactagat
    30541 cgagcttaat gatctgatcg gacagtttgg ttttcccggc ttccatcggg ccgccggaaa
    30601 caaagatcac cggaatattc aggcgcaggg aagccatcag catccccggg gtgattttgt
    30661 cgcagttaga gatgcagacc atggcgtcgg cgcagtgagc gttgaccata tactcaacgg
    30721 aatcagcgat cagttcgcga gatggcagtg aataaagcat ccccccgtgg cccatggcaa
    30781 tcccatcatc caccgcaatg gtgttgaact ctttggcaac gccgccagcc gcttcaattt
    30841 gttctgcgac cagtttaccg agatcgcgca gatggacgtg acccggtaca aattgggtga
    30901 acgagttcgc aaccgcgata atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc
    30961 gccacagcgc acgagcaccc gccatattac gaccatgagt ggtggtggcg gaacggtact
    31021 taggcatact ttatttactc ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc
    31081 attttttgta tttattgatt aacttgatct aaccagcccc atttatcttc ggtttcgcca
    31141 gtgaagaggc cgaagaaggc ttgctgaatg cgtttggtaa ccgggccgca acggccttca
    31201 ccaacctgaa taccatctac actgcgcact ggcgtgattt ctgccgccgt accggacata
    31261 aacacttcat ccgccagata cagggattcg cgcgacagca cctgctcacg cacttcaatt
    31321 cccaactctt tcgccagttt gatgatggcg tcacgggtaa tacccggcag cgcggaggag
    31381 gtgaacggtg gggtgaacag cacaccatct ttcacttcaa acaggttttc gcctgcgcct
    31441 tcagagatat aaccgttcac atccagcgcg ataccttcct gataaccgtg gcggcgcgct
    31501 tcgctaccca ccagcaggga agagaggtag ttaccaccgg cttttgccgc cgtcgggatg
    31561 gtgtttggtg ctgcgcggtt ccaggaggaa accatcgcat cgatcccctg ctccagcgct
    31621 tctgcgccca gatacgctcc ccacgggaaa gcggcgataa tcacatcggt tgagtatccc
    31681 gctggcgggt taacgcccat accaacatca ccgacgaaga tcagcggacg gatataggcg
    31741 ctggtgagat tgtttttgcg gatcacgtca cgacaagctt ccatcagctc atcaatgctc
    31801 tgcgaaaccg ggaagcgata gattttggcg gagtcatgca gacgctgcat atgctcacga
    31861 tggcggaata caaccggtcc tttgtgcgag tcgtagcaac ggatgccttc aaaaaccgag
    31921 gtgccatagt gcagcgcgtg cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc
    31981 tccccattga accaaatgta atcagctttc ttcgtggtca tttttatatt ccttttgcgc
    32041 tcaggcgcgg atttgttgtg atgtggttgt gctctggcag atggcaacgt gtgcgacgtc
    32101 caccagttta tttaactgac taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa
    32161 ttcgatattt atattttgtg catcgctggc ggcggccata ttcattgaac agacgtggaa
    32221 accacgatga cgcaccacgc gtaacacacg ttctaaggtt tccggattga agcgagccga
    32281 tacattgacc tgatgttgca tcatgataat ttctccaaca tttctgaatt actggcacca
    32341 ggcggcacca gcggccagac gttctcaaat tcgtcgattg agacatgaag caggtatggc
    32401 ccatcactgt tcagcatggt gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt
    32461 tggccaggga tgccgaaggc gctggctaac atgagaaaat cggggttatc ggtaagggtg
    32521 gtttcgctat atcgttcctg gaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt
    32581 tggttatcga gtaagacgat tttcaacggt aactgcttac gttttacggt gcccagctct
    32641 tgtacattca tcatgaaaga gccgtcaccg gagatacaga cgacggtatc gttcggtcgc
    32701 gcgacttgcg cgccaaccgc cgccggtaaa ccgaaaccca tggtgcctaa gccgctggag
    32761 gtgatgaaat tttccgggcg agtatgggcg atgtgctgcg cagcccacat ctggtgctgc
    32821 cccacatctg tggtcacgac gcaatccgca ggtttacgat ccgacagttg ttttaacaac
    32881 aatggcgcgt agatagcgtc accgggatgg tcgtaacgcc aggtatgttc atcacgcagc
    32941 tgcgcgcagt attgctgcca gtcattgata tttaacggct gctgtaatgc tggtaacaga
    33001 gcatttaaat caccttgtaa tgccacatgt gcctgacgca gcttgttcat ttctgccggg
    33061 tcgatatcca tatggataac actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc
    33121 cggtcatcaa aacgtgcacc cacggcgatc agcaagtcgc actcctgcac cgcgaagttc
    33181 gccgctttgg tgccatgcat tcccagcatg cccaggtagt acggataatc tgcttcaact
    33241 gcgcccagcc ctttcagcgt gcaggtggca ggcatttttg tggtagcgag aaattctcgt
    33301 aaagcaggaa ctgcctgcgc catacccacg ccaccaccaa cgtacagcat cggtttttgc
    33361 gcttttgcca gcatctggcg cgcttgctca acttcggcat gggggaaagt cacttcgttt
    33421 tcaacggtgg tgaaccacgg ttccagatcg ccgctggcta actggatatc ttttgggata
    33481 tcgaccagaa ccggaccagg acgacctgag ctggcaacgt cgaatgctgc agccatgatg
    33541 cgcggcaact cttccagcga ctgcaccaga aagctgtgct tggtacaggc taacgacaat
    33601 cccaggacat ccacttcctg aaatgcgtcg gtgccgataa acggtgcgga cacttgaccg
    33661 gtgatggcaa cgacagggat ggaatctaac agtgcgtccg caagcccggt tatcaggttg
    33721 gttgcgcccg gaccagacgt ggcgatacat acgccagttt taccggtagc acgggcataa
    33781 ccgatagccg ccattgccgc accctgctca tgtcggcaca gcaagtgctc cacgccgccg
    33841 tcatacaatg catcgtaaac cggcataatt gcgccacccg gataaccgaa aacggtgttc
    33901 acaccctgtg cccgcaacgc atgtaccacc cactgtgcgc cattcatagt tagttccccg
    33961 tcctgaatct tgagaaacag aattttgtgc tgttattcat tgtctgctcc tcggttatgt
    34021 ttttaaggtc aaaaaaaacc cccggacctt tcggtgcggg ggtcttagtt cgttaaggct
    34081 tgatttctaa gcctttcctc gtccaagtgc agccccgcac ggtgggataa taatcaccac
    34141 cacgctaatc acgaccaggc taatcactcg tagaagggct gtcattttgt cttttcttgc
    34201 atcttgttcg aaggaatgcc taaagagtta ccataggttt tgtaaatagt acaagatatt
    34261 ttttggccga tttttatgat gcgtttaata atttcaatat aaattgttgt tttttaaaaa
    34321 taaaatagtg agtgaaaaaa attcaatttc agtatgaatg ttgttccctt cctcgtagtt
    34381 catcctttgt ccgctgcatt tttgcgagca tcattccacc gccagaacaa ctcaacttca
    34441 cttgcacaaa taaacaggaa tcagccacta aaattcacga attagcgggc tacagcagcg
    34501 atatcttatg cacataatgg tgactcaagg agggcttatg tcactgtcaa ttgttcatac
    34561 ccgcgcagcc ctgggagtaa atgcgccccc gatcactgtt gaggtacata tcagtaaagg
    34621 tctacccggc ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag ctcgcgatcg
    34681 cgtgcgcagc gccattatca atagcggata tgaatatccg gccaaaaaaa tcaccatcaa
    34741 cctggctcca gccgatctgc caaaagaagg gggacgatat gatttaccta tcgccattgc
    34801 gttgctggcg gcctcagaac agcttacagc caataagtta gatgaatatg aattagtcgg
    34861 agaactggcg cttacaggcg ctctgcgtgg cgttcccggt gcaatctcca gtgcaactga
    34921 agctattaag tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct
    34981 aattaacggt gaaggatgcc tgatagccga tcatctgcgg gctgtctgtg cgtttctgga
    35041 aggtaagcac gctctcgaac gcccgaaacc aactgatgca gtatcccggg cgctacaaca
    35101 tgatctcagt gatgttgtcg gtcaggagca aggaaagcga ggactggaaa ttaccgccgc
    35161 tggcgggcac aaccttttac tgattgggcc gccgggaaca ggtaaaacaa tgctcgccag
    35221 ccgtattaat ggcctgttgc cagatttaag caatgaagag gcactggaga gcgctgcgat
    35281 attaagtctg gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc
    35341 accgcatcac agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc cagggccagg
    35401 tgaaatttcg ttggcgcata acggcgtgct ttttcttgat gagctacctg aatttgaacg
    35461 gcgtacactg gatgccttgc gagagccgat tgaatccggg cagatccatc tttcacgcac
    35521 acgagccaaa ataacctatc cagcccgttt ccagcttgtt gcggcgatga atcccagccc
    35581 taccggacat tatcagggaa accataaccg ctgcacgcca gaacagacat tacgttatct
    35641 caaccggctc tcggggccct ttctcgaccg cttcgatctc tcactggaga tcccattacc
    35701 accccccggc attttgagta aaacggtagt gccgggagaa agcagcgcca ccgttaaaca
    35761 acgcgtaatg gccgccagag agcgccaatt taagcggcag aataaactga atgcctggct
    35821 ggatagtccg gaaatacgcc aattctgcaa acttgagagc gaagatgcga tgtggttgga
    35881 aggaacactg atccatctgg ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc
    35941 acgaaccatt gctgatattg atcagtctga cattatcaca cgtcagcatt tgcaggaggc
    36001 agttagctat cgtgcgattg accgtttgct catccatctg caaaaactac tgacataaaa
    36061 aaagggcatt tcgccctttt tattaatcat cagaatcggt gtagtcttca gcaccttcaa
    36121 cctgcggttt accgccggaa agggtgtgaa aacgttttgg acgcttaata cgcgtcatat
    36181 acttggacca cacgcgttct gcttctgtca ctggctcacg ttcgccacgg catactgcta
    36241 cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg
    36301 cataaccatg acgctcaagc agttgtgcct ctttgatggt gaaatcacca tgacgagaga
    36361 atccacgtgg ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca
    36421 tcctgcacgc tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt
    36481 gacagagtgt aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg
    36541 tggatacgga attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag
    36601 cggctgaatc gctctatctg acccagtcag cagtgagctt tcgaatcaga caactggaaa
    36661 atcaactggg tgtgaacctt ttcacccgcc acagaaacaa tatccgttta accgctgccg
    36721 gtgaaaaact actgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgcaagg
    36781 aggtggcgca tacctcacga cataacgagt tttctatcgg tgccagcgcc tcattgtggg
    36841 aatgtatgct taatcagtgg ctgggacgtt tgtatcaaaa tcaggatgcc catacaggct
    36901 tacagttcga agcgcgaatt gcccaacggc agtctctggt aaaacagctg catgaacgcc
    36961 agcttgatct tcttattacc actgaagcgc ccaaaatgga cgaatttagt agtcagttgc
    37021 tgggatattt cactttagcg ctttatacca gtgccccttc aaaactaaag ggagatctta
    37081 attatctgcg acttgagtgg gggccagatt ttcaacagca tgaggcaggt ttgatcggtg
    37141 ctgacgaagt gcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga
    37201 tgcttaatgg ttgcacctgg ctacccgtca gctgggcgcg taaaaaaggc ggcctgcata
    37261 ccgttgtcga tagcacaaca ctttcacggc cgctttatgc catatggctg caaaatagcg
    37321 ataaaaatac gttgattcgc gatcttttga aaattaacgt gctggatgaa gtgtattaat
    37381 atgaatggct ggcaaggatg ccggtagaag gatttacttc ggagagggtt atttcagata
    37441 aaaaaaatcc ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc
    37501 ccaacacccg gttttggaga ccggtgctct accaattgaa ctacgcccct aattagggtg
    37561 gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccg
//
LOCUS       DAPHDX010000034        37383 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPHDX010000034 DAPHDX010000000
VERSION     DAPHDX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.034"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            259..1632
                     /gene="aroP"
                     /locus_tag="RH058_001655"
     CDS             259..1632
                     /gene="aroP"
                     /locus_tag="RH058_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV3137253.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAIFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1675..2529)
                     /gene="ampE"
                     /locus_tag="RH058_001656"
     CDS             complement(1675..2529)
                     /gene="ampE"
                     /locus_tag="RH058_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV3137254.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAALFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2526..3077)
                     /gene="ampD"
                     /locus_tag="RH058_001657"
     CDS             complement(2526..3077)
                     /gene="ampD"
                     /locus_tag="RH058_001657"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV3137255.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3165..4058
                     /gene="nadC"
                     /locus_tag="RH058_001658"
     CDS             3165..4058
                     /gene="nadC"
                     /locus_tag="RH058_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV3137256.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4261..4701
                     /gene="ppdD"
                     /locus_tag="RH058_001659"
     CDS             4261..4701
                     /gene="ppdD"
                     /locus_tag="RH058_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV3137257.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4711..6096
                     /gene="gspE"
                     /locus_tag="RH058_001660"
     CDS             4711..6096
                     /gene="gspE"
                     /locus_tag="RH058_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV3137258.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6086..7288
                     /gene="hofC"
                     /locus_tag="RH058_001661"
     CDS             6086..7288
                     /gene="hofC"
                     /locus_tag="RH058_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV3137259.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTEKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7323..8366)
                     /gene="guaC"
                     /locus_tag="RH058_001662"
     CDS             complement(7323..8366)
                     /gene="guaC"
                     /locus_tag="RH058_001662"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV3137260.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8522..8566)
                     /locus_tag="RH058_001663"
     CDS             complement(8522..8566)
                     /locus_tag="RH058_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137261.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8591..9211
                     /gene="coaE"
                     /locus_tag="RH058_001664"
     CDS             8591..9211
                     /gene="coaE"
                     /locus_tag="RH058_001664"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV3137262.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9211..9954
                     /gene="zapD"
                     /locus_tag="RH058_001665"
     CDS             9211..9954
                     /gene="zapD"
                     /locus_tag="RH058_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV3137263.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9964..10161
                     /gene="yacG"
                     /locus_tag="RH058_001666"
     CDS             9964..10161
                     /gene="yacG"
                     /locus_tag="RH058_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV3137264.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10261..10650)
                     /gene="mutT"
                     /locus_tag="RH058_001667"
     CDS             complement(10261..10650)
                     /gene="mutT"
                     /locus_tag="RH058_001667"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV3137265.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10710..13415)
                     /gene="secA"
                     /locus_tag="RH058_001668"
     CDS             complement(10710..13415)
                     /gene="secA"
                     /locus_tag="RH058_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV3137266.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13477..13989)
                     /gene="secM"
                     /locus_tag="RH058_001669"
     CDS             complement(13477..13989)
                     /gene="secM"
                     /locus_tag="RH058_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV3137267.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14220..15137)
                     /gene="lpxC"
                     /locus_tag="RH058_001670"
     CDS             complement(14220..15137)
                     /gene="lpxC"
                     /locus_tag="RH058_001670"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV3137268.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15238..16389)
                     /gene="ftsZ"
                     /locus_tag="RH058_001671"
     CDS             complement(15238..16389)
                     /gene="ftsZ"
                     /locus_tag="RH058_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV3137269.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16450..17712)
                     /gene="ftsA"
                     /locus_tag="RH058_001672"
     CDS             complement(16450..17712)
                     /gene="ftsA"
                     /locus_tag="RH058_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV3137270.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17709..18539)
                     /gene="ftsQ"
                     /locus_tag="RH058_001673"
     CDS             complement(17709..18539)
                     /gene="ftsQ"
                     /locus_tag="RH058_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV3137271.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18541..19461)
                     /gene="ddlB"
                     /locus_tag="RH058_001674"
     CDS             complement(18541..19461)
                     /gene="ddlB"
                     /locus_tag="RH058_001674"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV3137272.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19454..20929)
                     /gene="murC"
                     /locus_tag="RH058_001675"
     CDS             complement(19454..20929)
                     /gene="murC"
                     /locus_tag="RH058_001675"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV3137273.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20983..22050)
                     /gene="murG"
                     /locus_tag="RH058_001676"
     CDS             complement(20983..22050)
                     /gene="murG"
                     /locus_tag="RH058_001676"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV3137274.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22047..23291)
                     /gene="ftsW"
                     /locus_tag="RH058_001677"
     CDS             complement(22047..23291)
                     /gene="ftsW"
                     /locus_tag="RH058_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV3137275.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23291..24607)
                     /gene="murD"
                     /locus_tag="RH058_001678"
     CDS             complement(23291..24607)
                     /gene="murD"
                     /locus_tag="RH058_001678"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV3137276.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFAGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24610..25692)
                     /gene="mraY"
                     /locus_tag="RH058_001679"
     CDS             complement(24610..25692)
                     /gene="mraY"
                     /locus_tag="RH058_001679"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV3137277.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25686..27044)
                     /gene="murF"
                     /locus_tag="RH058_001680"
     CDS             complement(25686..27044)
                     /gene="murF"
                     /locus_tag="RH058_001680"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV3137278.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27041..28528)
                     /gene="murE"
                     /locus_tag="RH058_001681"
     CDS             complement(27041..28528)
                     /gene="murE"
                     /locus_tag="RH058_001681"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV3137279.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28515..30281)
                     /gene="ftsI"
                     /locus_tag="RH058_001682"
     CDS             complement(28515..30281)
                     /gene="ftsI"
                     /locus_tag="RH058_001682"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV3137280.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30297..30662)
                     /gene="ftsL"
                     /locus_tag="RH058_001683"
     CDS             complement(30297..30662)
                     /gene="ftsL"
                     /locus_tag="RH058_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV3137281.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30659..31600)
                     /gene="rsmH"
                     /locus_tag="RH058_001684"
     CDS             complement(30659..31600)
                     /gene="rsmH"
                     /locus_tag="RH058_001684"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV3137282.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31602..32060)
                     /gene="mraZ"
                     /locus_tag="RH058_001685"
     CDS             complement(31602..32060)
                     /gene="mraZ"
                     /locus_tag="RH058_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV3137283.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32662..33666)
                     /gene="cra"
                     /locus_tag="RH058_001686"
     CDS             complement(32662..33666)
                     /gene="cra"
                     /locus_tag="RH058_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV3137284.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33748..33834)
                     /locus_tag="RH058_001687"
     CDS             complement(33748..33834)
                     /locus_tag="RH058_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV3137285.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33846..34337)
                     /gene="ilvN"
                     /locus_tag="RH058_001688"
     CDS             complement(33846..34337)
                     /gene="ilvN"
                     /locus_tag="RH058_001688"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV3137286.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34340..36064)
                     /gene="ilvI"
                     /locus_tag="RH058_001689"
     CDS             complement(34340..36064)
                     /gene="ilvI"
                     /locus_tag="RH058_001689"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV3137287.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36183..36227
                     /locus_tag="RH058_001690"
     CDS             36183..36227
                     /locus_tag="RH058_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137288.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36382..37326)
                     /gene="leuO"
                     /locus_tag="RH058_001691"
     CDS             complement(36382..37326)
                     /gene="leuO"
                     /locus_tag="RH058_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV3137289.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         8974 a        10444 c         9147 g         8818 t
ORIGIN      
        1 acgaatttaa attcattcta catattgaga ggggttgagg ctgagcttta caaacggttt
       61 ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca
      121 ttattcttga tctgacggaa gtctttttgt aacaattcaa acttctttga tgtaaacaaa
      181 ttaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca
      241 accaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc
      301 cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac cgggttattc
      361 ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt
      421 gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct
      481 gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc
      541 tctggctgga actactgggt actgtacgtt ttagttgcca tggctgagct gactgccgtg
      601 ggtaaataca ttcagttctg gtatccggaa atccccacct gggtttctgc cgccatattc
      661 tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc
      721 tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga tcatcttcgg cggctggctg
      781 ctattcagtg gcaacggcgg cccgcaggcg accgttagca acctgtggga tcagggtggt
      841 ttcctgccgc acggcttcac cgggctggtg atgatgatgg cgattatcat gttctcgttc
      901 ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtata
      961 ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc
     1021 gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg
     1081 atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact
     1141 gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg
     1201 gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg ataaacgtgg tgtaccagta
     1261 aataccattc tggtgtctgc actggtaacg gcgttgtgcg tactgattaa ctaccttgcc
     1321 ccagagtccg ctttcggact gttaatggcg ctggtggtat ctgcactggt aatcaactgg
     1381 gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca agcaggaaca aggcgtggta
     1441 actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg
     1501 gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg
     1561 gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta
     1621 aaagcgcatt aatctctcta cgccctcacc cgtacagggt gagggcaata atctttacac
     1681 caacgccccg taaatcgtca gtagtgcaat caccaccacg accacgaacg aggttttctt
     1741 cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag
     1801 agagaactgc gccagacgcg ttaacacctg atactgcgaa gtatggaaat cacccagcga
     1861 agcaaaccag gccggtaacg ctttctcacc atgaccgatc aaggcatata ccacacccgc
     1921 aagacgaacc ggcacccaat ccagtacatg aagcacggca tcaatgccgg actgtaaacg
     1981 atgatgcggc gtctgatatc gcgccagcca gtattgccat gcacgcaaaa acgcataccc
     2041 catcagcgta acgggtcccc aggttccccc cacaatcagc cagaacagcg ctgcaagata
     2101 aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcgtc
     2161 gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg cacgggcatg
     2221 gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc
     2281 gccaatacac agcaaaccaa tcagcagcca caccagtagc gtgggaacgt tgaacaatac
     2341 tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg tcatgcctaa
     2401 cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag
     2461 ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa
     2521 tagcgtcatg ttgtctcctt gctgaccagc acacgaaacc gtgcccaatc aaatgcagga
     2581 ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt tttagcgata
     2641 tccggatagc aatcaatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg
     2701 gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt
     2761 tcgcgcccct gatactgaga gactcccgca tgccatgcac gtttatcgaa aggaacatac
     2821 tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc
     2881 tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac
     2941 ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt
     3001 tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac
     3061 ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta
     3121 gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct
     3181 ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg
     3241 cggtagccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgatatta
     3301 cggcaaaact tttaccggaa aattctcgct ctcatgccac ggtgatcacc cgcgagaatg
     3361 gcgtcttttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg
     3421 tcaccataat ctggcatgtg gatgacggcg atgtcatcaa tgccaatcaa tccttgttcg
     3481 aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc
     3541 aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattgctg gaaggcacca
     3601 acacgcagtt gttggatacg cgcaaaacct tacccggcct gcgttcagct ctgaaatacg
     3661 cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca
     3721 aagaaaacca tattattgcc tccggctcag tgcgccaggc ggtcgaaaaa gcgtcctggc
     3781 tgcacccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaagccc
     3841 tgaaagcagg agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag
     3901 ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa
     3961 cactgcgtga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg ctaactaaac
     4021 acgtacaagc actcgacctt tcaatgcgtt ttcgctaata gttgacagat ttatccgctc
     4081 catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt
     4141 gtctttcgct gcaacgaaat aaaaaacctc ggaatctgct ttcccgtttc gttttttgcc
     4201 cctcgcctgt gctgatggcg tttgccaaag tagcgccaac caaatcaagg agcgaaacag
     4261 atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc
     4321 attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc cgcactcacc
     4381 gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat
     4441 ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac caccacccac
     4501 tatgtttcag ccatgagtgt ggcaaaaggc gtggtgtcgc tgaccgggca agaaagtctc
     4561 aatggactaa gcgtcgtcat gacaccgggt tgggataacg caaacggcgt caccggctgg
     4621 acgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga agatgtcttc
     4681 cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac ggccctgtgt
     4741 ctgcgttatc agggagtctt gctggatgcc agcgaagaag tggttcatgt tgcggtggtc
     4801 gatgccccct cacatgagtt gctggacgca ttgcatttcg ctaccaccaa acgtattgag
     4861 atcacctgct ggacgcgcca acaaatggaa ggtcacgcca gtcgcacaca acagacattg
     4921 cccgtagctg ttcaggagaa gcatcagccc aaagcagagt tgctagctcg aacgttacaa
     4981 tctgcgctgg aacaacgcgc gtctgatatt catatcgaac cagcggacaa tgcctaccgc
     5041 atccgcttgc gtatcgacgg cgtattgcat cctttaccgg atgtttcacc ggatgccgga
     5101 gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg atattgcgga acatcgcctg
     5161 ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg ccgtctcatt tcgtattgcg
     5221 accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca ggtgggtcag
     5281 gcactggatg tcaacacgct tggaatgcag ccgttacaac tggcggactt tgctcatgcc
     5341 ttgcaacaac cacagggact ggtgctggta actggcccta ccggcagcgg caaaacggtc
     5401 acgctttata gtgccctgca aacgctgaat accgctgaca ttaatatttg tagcgtcgaa
     5461 gatccggttg agatccccat agccggacta aaccagacgc aaatccatcc gcgtgccggg
     5521 ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg atcctgacgt catcatgatc
     5581 ggagagatcc gcgatggcga aacagcagag atcgctatta aagcggcgca aactggtcac
     5641 ctggtgttgt ctaccctaca cactaattcc acctgcgaaa cgctggtacg tttacagcaa
     5701 atgggggtcg cccgctggat gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg
     5761 gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg agcccatcca tattccagtc
     5821 aatgtatggc cgtcgccgct gccccactgg caggcacccg gttgtgtaca ttgctaccac
     5881 ggtttttatg gtcgtacggc cttatttgaa gttctgccca taacgccggt cattcgtcag
     5941 cttatttccg ctaataccga cgttgaatcg ctggaaacgc acgcacgaca ggcgggtatg
     6001 tgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag gcttaaccac ctttgaagag
     6061 ttaatccgcg tactggggat gccgcatggc gagtaagcaa ctctggcgct ggcatggcat
     6121 aaccggcgac ggcaatgcgc aagatgggat gctatgggca gagagccgtg ctttgctgct
     6181 catggcacta cagcaacaga tggttacccc acttagcctg aagcgaatcg ccatcaattc
     6241 tgcgcagtgg cgaggagata aaagcgcgga agtcattcat caactggcga cgctactcaa
     6301 agccgggtta acgctttctg aagggctggc actgctggcg gaacagcatc ccagtaagca
     6361 atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa cagggcattg ctttttccaa
     6421 tgccttatta ccctggtcag aggtatttcc gccactctat caggcgatga tccgcacggg
     6481 tgaactgacc ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc aaaaagccca
     6541 gcgtcagttg accgaaaaag tgaaatcagc gttgcgctat cccatcatca ttttagcgat
     6601 ggcaatcatg gtggttgtgg caatgctgca ttttgttctg ccggagtttg ccgctatcta
     6661 taagaccttc aacaccccac taccggcact aacgcagggg atcatgacgc tggcagactt
     6721 tagtggcgaa tggggctggc tgctggtgtt gttcggcttt ctgctggcga tagccaataa
     6781 gttgctgatg cgccgcccta cctggcttat cgcgcggcag aaattgttgt tacgaatccc
     6841 gattatgggt tcactgatga ggggacaaaa actcacgcag atctttacga ttctggcgct
     6901 gacacaaagt gcaggcatta cttttttaca gggcgtagag agcgtcagag aaacaatgcg
     6961 ctgcccgtac tgggtgcaac ttctgacaca aatccagcac gatatcagta acggtcatcc
     7021 catctggctg gcgctaaaaa atgccggtga gtttagcccg ctctgtttgc aattagtgag
     7081 aacgggagag gcatccggct cgctggatct catgttagac aacctcgccc atcatcatcg
     7141 ggataacaca atggcgctgg cggataacct cgcagcctta ctggaaccgg cgttgctgat
     7201 cataacggga ggaattatcg gtacgctggt ggtggcgatg tatctgccaa ttttccattt
     7261 aggcgatgcg atgagtggga tgggataacg ctggcgtgtt gctccacgcc agcgttggga
     7321 gattacaggt tgttgaagat gcggttttct tgttcctgca cccgaataaa cgtggtgcgc
     7381 ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac aagctgaacg caagccgccc
     7441 aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg gcagcttaac ggttttacct
     7501 tctgctgcgc gatattccgc aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc
     7561 atgccgtaga acagcataaa tttctcgccg ttctcctcaa cgatgcgacc gccgctctct
     7621 tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg caccgccgcc aaaggctttt
     7681 gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga tcattccgcc cagaccgtgc
     7741 gccgcatcgg cacattcgat taccgcagaa agctgcggat aaccgacgcc tgttttgacg
     7801 cgagttgtac aaacagaacc tgggccaatg ccaactttaa cgatatcagc accggagagg
     7861 ataagctcct cacacatttc accagtcact acgttgccag cacaaatggt tttggtcggc
     7921 cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt cggaataacc attcgccaca
     7981 tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa
     8041 tccgcatcag acgtaccggt agaaaccatc acatgtttca gcacatcagc ggaagaattg
     8101 ttgataaacg cttgccactc ttcgacagaa tagtgtttat gcacagcagt caaaatatca
     8161 aaagaagcca gcgcagaggc catagaaaat gtacctacgg tgtccatatt tgcggcgata
     8221 attggcacgc cggaccagct ctgacctgaa tgtttgaagg tgaattgacg ttccagttca
     8281 acatcggaac ggcttttaag agtggagcgt ttagggcgga tgagaacgtc tttaaaacct
     8341 aacttcagat cttcttcaat acgcatgtgc ggattcctgg ggttaatggc tataagggta
     8401 agagcgcaac tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa
     8461 ttgtcttttt ttacgctaca atcccataaa tttacctggt aaatagtgag cgaacgtttc
     8521 atcaatttcg ctttcgtgca ctcattttta acgttttgat aatcatgatg aattcccggg
     8581 aatataaatt atgaggtata tagttgcctt aacgggaggc attggcagtg gcaagagtac
     8641 cgttgccaat gcgtttgctg atctcggaat taacgtcatt gatgccgata ttattgcgcg
     8701 tcaggtggtt gaaccaggtg cacctgcgct acatgccatt gctgatcact ttggcgctaa
     8761 catgattgct gctgatggaa cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa
     8821 cccggaagag aaaaactggc ttaacgccct gctgcatccg ctgattcagc aagagacgca
     8881 acaccagatc cagcaagcta cttcccccta tgtactgtgg gttgtgccat tgctggtaga
     8941 aaactcactg tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca
     9001 acttaagcgc accatgcagc gcgatgatgt aactcgcgag catgtcgaac aaatccttgc
     9061 tgctcaggca acgcgcgaag cccgccttgc cgtggcagat gacgtcattg ataataacgg
     9121 cgcaccggat gctatcgcat cggatgttgc ccgcctgcac gcacactatt tgcagcttgc
     9181 gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc aggtcctttt tgaacatcca
     9241 ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt tgattcagca actcaccgtt
     9301 aatttaccca tcgttgacca cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta
     9361 ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt tgaaagaact tgaccggcag
     9421 caacgtaaac tccagacctg gattggcgtg cctggcgtgg accagagccg tattgaagca
     9481 ttaattcagc agttaaaagc ggcggggagc gtattaattt ccgcgccgcg tatcgggcaa
     9541 tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc gactgagcat cccaggcggc
     9601 tgttgcagct ttgatttacc tacattgcac atttggctgc atctacccca ggcgcagcgc
     9661 gacagccagg tagaaacctg gattgccagc ctgaacccgc tcacccaggc acttaccatg
     9721 gtgctggatt taattcgcca gtcggctccc ttccgtaaac aaaccagcct gaatggtttt
     9781 tatcaggata acggtggcga tgccgacttg ctgcgcctga atctgtcgct cgattcacag
     9841 ctttatccgc aaatttccgg acataagagc cgttttgcca ttcgttttat gccgctggac
     9901 actgaaaacg gacaggtacc ggaacgtctg gatttcgaac tggcctgttg ctaaggagtg
     9961 aaaatgtcag aaactattac ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt
    10021 gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc agctgatcga cctcggagaa
    10081 tgggctgctg aagaaaaacg aatccccagc agtggcgatc tttccgaaag cgatgactgg
    10141 agcgaagagc caaagcagtg acatttgtct gatgccgcac gtaggcctga taagacgcgg
    10201 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
    10261 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
    10321 aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
    10381 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
    10441 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
    10501 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
    10561 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
    10621 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
    10681 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
    10741 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
    10801 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
    10861 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
    10921 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
    10981 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
    11041 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
    11101 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
    11161 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
    11221 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
    11281 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
    11341 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
    11401 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
    11461 gccacgtcat catattccag cagttgctta cgaatgtcga agttacggct ttcaacttta
    11521 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
    11581 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
    11641 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
    11701 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
    11761 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
    11821 tctgcggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
    11881 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
    11941 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
    12001 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
    12061 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
    12121 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
    12181 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
    12241 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
    12301 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
    12361 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
    12421 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
    12481 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
    12541 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
    12601 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
    12661 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
    12721 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
    12781 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
    12841 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
    12901 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
    12961 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
    13021 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
    13081 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
    13141 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
    13201 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
    13261 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
    13321 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
    13381 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
    13441 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
    13501 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
    13561 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
    13621 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
    13681 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
    13741 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
    13801 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
    13861 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
    13921 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
    13981 tccactcacg ttattgccat cccgatcata ttcaaacgta tttagcgcac aaatcttatc
    14041 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
    14101 aacagcgttc gcaccgtttt ttatagctct tataaaattc aaaaaaaaac gactggttca
    14161 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
    14221 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
    14281 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
    14341 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
    14401 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
    14461 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
    14521 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
    14581 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
    14641 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
    14701 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
    14761 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
    14821 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
    14881 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
    14941 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
    15001 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
    15061 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
    15121 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
    15181 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
    15241 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
    15301 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
    15361 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
    15421 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
    15481 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
    15541 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
    15601 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
    15661 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
    15721 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
    15781 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
    15841 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
    15901 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
    15961 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
    16021 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
    16081 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
    16141 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
    16201 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
    16261 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
    16321 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
    16381 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
    16441 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
    16501 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
    16561 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
    16621 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
    16681 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
    16741 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
    16801 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
    16861 cggacgaccg cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
    16921 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
    16981 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
    17041 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
    17101 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
    17161 ttggtcaact ttcagtccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
    17221 gtgacatgtg atcaggtgca cttttgcctg catcctcacg cccgaaagtc ctaccggatt
    17281 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
    17341 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
    17401 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
    17461 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
    17521 ggcacgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
    17581 acgcgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
    17641 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
    17701 cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc
    17761 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
    17821 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
    17881 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
    17941 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
    18001 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
    18061 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
    18121 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
    18181 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
    18241 tctatttgcg tctgaatgat gttgacatcc tgggtcataa aggtacccgg ctcacccaat
    18301 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
    18361 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
    18421 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
    18481 ttgcggcgag aagaaacctc ttcttcactg tttcgcgtgt tcagagcagc ctgcgacata
    18541 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
    18601 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
    18661 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
    18721 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
    18781 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
    18841 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
    18901 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
    18961 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
    19021 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
    19081 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
    19141 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
    19201 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
    19261 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
    19321 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
    19381 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
    19441 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
    19501 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
    19561 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
    19621 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
    19681 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
    19741 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
    19801 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
    19861 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
    19921 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
    19981 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
    20041 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
    20101 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
    20161 cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    20221 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    20281 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    20341 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    20401 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    20461 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    20521 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    20581 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    20641 cgacaatttc cgggttatcg gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    20701 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    20761 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    20821 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    20881 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    20941 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    21001 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    21061 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    21121 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    21181 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    21241 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    21301 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    21361 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    21421 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    21481 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    21541 cggcagcgcc agtacatcgg tacgcaccgg attacccacc acttccgcat tagggaaagc
    21601 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    21661 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    21721 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    21781 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    21841 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    21901 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    21961 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    22021 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    22081 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    22141 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    22201 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    22261 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    22321 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    22381 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    22441 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    22501 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    22561 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    22621 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    22681 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    22741 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    22801 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    22861 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    22921 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    22981 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    23041 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    23101 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    23161 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    23221 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    23281 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    23341 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    23401 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    23461 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    23521 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    23581 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    23641 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    23701 gatgcggcag accggcgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    23761 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    23821 tcatctcttt cacgttcagc actttctcgc ctttcactcg cagccaggtt tctccctgct
    23881 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatcagcac
    23941 cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc gcattttcgt
    24001 aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg tccatatgat
    24061 cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct
    24121 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    24181 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    24241 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    24301 cttcgcgaca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    24361 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    24421 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    24481 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    24541 ccacgcagga aagcccggtg aggcccaggc cgataatgac gacattttta ccctgataat
    24601 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    24661 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    24721 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    24781 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    24841 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    24901 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    24961 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    25021 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    25081 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    25141 aatgacgaag taagccagca gaatgtagaa cagccccagt tgcggcatca catctttaaa
    25201 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    25261 gacacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    25321 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    25381 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    25441 cactatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    25501 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    25561 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    25621 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    25681 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    25741 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    25801 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    25861 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    25921 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    25981 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    26041 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    26101 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    26161 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    26221 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    26281 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    26341 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    26401 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    26461 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    26521 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    26581 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    26641 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    26701 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    26761 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    26821 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    26881 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    26941 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    27001 ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    27061 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    27121 cttcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    27181 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    27241 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    27301 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    27361 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    27421 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    27481 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttca
    27541 gcagatcagc cagtggatag ccgagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    27601 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgagctaa
    27661 agcgaatcgt cgcaccgctg tcgtgatagt tcacgtcgat cgcttttaac cagcgtccgt
    27721 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    27781 gccagcggcg gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag
    27841 aataaagcag ccatttcgca gcttcgtagt gttccatatc accatgataa tcaaggtgat
    27901 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    27961 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgctcc accagccccg
    28021 ccagctcatg ctgaacatcg actgccgaac cggtagtatt ttctgtcggg atcactttcc
    28081 caagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    28141 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    28201 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    28261 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcacca tcggtcgcct
    28321 catctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc
    28381 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    28441 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    28501 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    28561 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    28621 atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga
    28681 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    28741 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    28801 ttaatggcga tacgatagcc tttaatcgcc gctttcacgc cgccgccgcc tggtagcgcc
    28861 acgctttcca tcatatgcac cacggtgcga acaatggatt ccgggaagac acgttcaccg
    28921 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    28981 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    29041 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    29101 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    29161 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    29221 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    29281 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    29341 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    29401 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    29461 gccagcactt caccggtgtt gacatccacc agcacggcgc taccggattc agccttgtta
    29521 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaagcgttc atcaatactc
    29581 aacgccaggt tgtgcgccgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    29641 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    29701 ttctcaacgc cctcaatccc ctgactatcg acgttagtaa agccgatgag gtgagcagtc
    29761 acttcgccgg atggataata acggcgagat tcttcacgca gatgaatccc cggcagtttc
    29821 aattttttga tgtagtccgc catgtcaggg tttacctgac gcgccagata aataaagcgc
    29881 cctttcgggt tggcgttaat gcgggcagaa agctgatcca gcggaatatt gagcgcgtta
    29941 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    30001 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    30061 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    30121 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    30181 gccaggagaa tacagccgca taacaacgca aaacgccaac taataaagtt ggcatgttct
    30241 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    30301 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    30361 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    30421 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    30481 cgggtatggt gcgccgtggt taccacggtc accgccgtca aaataatgca aatgaacagg
    30541 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    30601 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    30661 atgcgttcgt cctctctgcg atacgcagaa ctgaactacg ggcacgagga ttctcagcca
    30721 cctcttcttc gcccggcatt aacttgccta atgctcgcag ctgacggcca cccagttttt
    30781 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    30841 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    30901 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    30961 tgtttaccca aatgcgcacc gcctggaagg tacgagtcgc gggatgttta aacttatctt
    31021 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    31081 cgcgattacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    31141 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    31201 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    31261 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    31321 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    31381 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    31441 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    31501 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    31561 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    31621 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    31681 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    31741 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    31801 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    31861 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa
    31921 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    31981 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    32041 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    32101 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    32161 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    32221 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    32281 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    32341 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    32401 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    32461 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    32521 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    32581 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    32641 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcgacgat agagattacg
    32701 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    32761 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgccagca ccgggcactg
    32821 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    32881 accatcgcga cgtagcgtta catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    32941 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    33001 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    33061 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    33121 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    33181 ggcaccaacc acgctggtga agtgttcacg atcgagggcg cggtccagcg cgacaatcgg
    33241 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggaggcaacg acgtcgaaac
    33301 aataatggca tcgacctgac gctgtaaaag gtgctcaata cagcgcattt cgttgtctgg
    33361 ctgatcttct gagcaggcaa ttagcagttg ataaccccgt tgccgcgcct ggcgttcaag
    33421 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    33481 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    33541 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    33601 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    33661 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    33721 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    33781 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    33841 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    33901 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    33961 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    34021 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    34081 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    34141 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    34201 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    34261 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    34321 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    34381 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    34441 acaccaggcg attattgcgc acctgttcca gcgcctcgct aagtttgctt tccagctcat
    34501 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    34561 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    34621 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    34681 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    34741 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    34801 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagtgcag
    34861 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    34921 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    34981 actgacgagc gcgccactgt tcaatctgct gccaccagtc gcggatctca tccagcggtt
    35041 gatgggcgga ttcttgcgac aggagttcaa gcatttgttc gaggacctgg cgagcatccc
    35101 ccacaatcgg gatatcagca gtcacggttt tagaaatgga agtaggatca atatcgatat
    35161 gcagaacagt ggcatttggg cagtactttg ccagattatt cgtcgttcgg tcatcaaatc
    35221 ttaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtgc
    35281 cgtgcattcc cagcatgccc agcgcctgac gatgcgttgc cggaaatgcc cccaggccca
    35341 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    35401 agcccgccgt gattgcccca ccgcctacgt agacaaccgg tttttttgcc gccaccagcg
    35461 tttgcagagc acgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
    35521 tactaaccga ctccggccag acatagggta atttgttcgc cggattaaga atatctttcg
    35581 gtaaatcaac gactactggt ccaggacgac cacttgccgc cagccagaaa gcctttttca
    35641 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    35701 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    35761 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    35821 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcacgcg
    35881 ccaggccatc ggccatatgc accgccgcct gctcatgacg aactaataca tgatcaatac
    35941 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    36001 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    36061 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    36121 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
    36181 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgacaa taaatcggct
    36241 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa tagagagcac
    36301 acacattcag aataaaattc tacattcaat aaaaaatgca gaataaacca gacattcatg
    36361 tctggcctat tctgcattca gttagcgttt gcaaattgag actaattgtt cttccatcca
    36421 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctgtt
    36481 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
    36541 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
    36601 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
    36661 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    36721 cagtaacggg cccttaattg ttggatgatt tttgctggct accagcacca tttcatcttt
    36781 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    36841 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    36901 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    36961 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
    37021 accaggcaat tcattttgta ctagttgcaa tgcctgacga actgagccaa aaagttgaaa
    37081 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
    37141 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
    37201 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    37261 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
    37321 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    37381 taa
//
LOCUS       DAPHDX010000035        37007 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPHDX010000035 DAPHDX010000000
VERSION     DAPHDX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.035"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..840
                     /gene="potE"
                     /locus_tag="RH058_001692"
     CDS             <1..840
                     /gene="potE"
                     /locus_tag="RH058_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDV3137290.1"
                     /translation="VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFL
                     GLESACANTDVVENPERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFG
                     LAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
                     APVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVA
                     NVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKN
                     KHG"
     gene            complement(896..2536)
                     /gene="pgm"
                     /locus_tag="RH058_001693"
     CDS             complement(896..2536)
                     /gene="pgm"
                     /locus_tag="RH058_001693"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV3137291.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(2562..3107)
                     /gene="seqA"
                     /locus_tag="RH058_001694"
     CDS             complement(2562..3107)
                     /gene="seqA"
                     /locus_tag="RH058_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV3137292.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3292..4056
                     /gene="ybfF"
                     /locus_tag="RH058_001695"
     CDS             3292..4056
                     /gene="ybfF"
                     /locus_tag="RH058_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3137293.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4196..4489
                     /gene="ybfE"
                     /locus_tag="RH058_001696"
     CDS             4196..4489
                     /gene="ybfE"
                     /locus_tag="RH058_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV3137294.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            4629..5159
                     /gene="fldA"
                     /locus_tag="RH058_001697"
     CDS             4629..5159
                     /gene="fldA"
                     /locus_tag="RH058_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV3137295.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5369..5455
                     /gene="uof"
                     /locus_tag="RH058_001698"
     CDS             5369..5455
                     /gene="uof"
                     /locus_tag="RH058_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV3137296.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            5448..5894
                     /gene="fur"
                     /locus_tag="RH058_001699"
     CDS             5448..5894
                     /gene="fur"
                     /locus_tag="RH058_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV3137297.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5979..6305)
                     /locus_tag="RH058_001700"
     CDS             complement(5979..6305)
                     /locus_tag="RH058_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137298.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(6355..7761)
                     /gene="chiP"
                     /locus_tag="RH058_001701"
     CDS             complement(6355..7761)
                     /gene="chiP"
                     /locus_tag="RH058_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV3137299.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(8207..9871)
                     /gene="glnS"
                     /locus_tag="RH058_001702"
     CDS             complement(8207..9871)
                     /gene="glnS"
                     /locus_tag="RH058_001702"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV3137300.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(10074..12020)
                     /gene="nagE"
                     /locus_tag="RH058_001703"
     CDS             complement(10074..12020)
                     /gene="nagE"
                     /locus_tag="RH058_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV3137301.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            12353..13153
                     /gene="nagB"
                     /locus_tag="RH058_001704"
     CDS             12353..13153
                     /gene="nagB"
                     /locus_tag="RH058_001704"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV3137302.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            13213..14361
                     /gene="nagA"
                     /locus_tag="RH058_001705"
     CDS             13213..14361
                     /gene="nagA"
                     /locus_tag="RH058_001705"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV3137303.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            14370..15590
                     /gene="nagC"
                     /locus_tag="RH058_001706"
                     /gene_synonym="nagR"
     CDS             14370..15590
                     /gene="nagC"
                     /locus_tag="RH058_001706"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV3137304.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            15638..16390
                     /gene="nagD"
                     /locus_tag="RH058_001707"
     CDS             15638..16390
                     /gene="nagD"
                     /locus_tag="RH058_001707"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV3137305.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            16647..18311
                     /gene="asnB"
                     /locus_tag="RH058_001708"
     CDS             16647..18311
                     /gene="asnB"
                     /locus_tag="RH058_001708"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV3137306.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            18692..18768
                     /locus_tag="RH058_001709"
     tRNA            18692..18768
                     /locus_tag="RH058_001709"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18726..18728,aa:Met,seq:cat)
     gene            18777..18861
                     /locus_tag="RH058_001710"
     tRNA            18777..18861
                     /locus_tag="RH058_001710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18811..18813,aa:Leu,seq:tag)
     gene            18885..18959
                     /locus_tag="RH058_001711"
     tRNA            18885..18959
                     /locus_tag="RH058_001711"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18917..18919,aa:Gln,seq:ttg)
     gene            18994..19068
                     /locus_tag="RH058_001712"
     tRNA            18994..19068
                     /locus_tag="RH058_001712"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19026..19028,aa:Gln,seq:ttg)
     gene            19084..19160
                     /locus_tag="RH058_001713"
     tRNA            19084..19160
                     /locus_tag="RH058_001713"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19118..19120,aa:Met,seq:cat)
     gene            19209..19283
                     /locus_tag="RH058_001714"
     tRNA            19209..19283
                     /locus_tag="RH058_001714"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19241..19243,aa:Gln,seq:ctg)
     gene            complement(19437..20612)
                     /gene="ubiF"
                     /locus_tag="RH058_001715"
     CDS             complement(19437..20612)
                     /gene="ubiF"
                     /locus_tag="RH058_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV3137307.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            20758..22182
                     /gene="miaB"
                     /locus_tag="RH058_001716"
     CDS             20758..22182
                     /gene="miaB"
                     /locus_tag="RH058_001716"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV3137308.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLHLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            22335..23375
                     /locus_tag="RH058_001717"
     CDS             22335..23375
                     /locus_tag="RH058_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV3137309.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            23372..23839
                     /gene="ybeY"
                     /locus_tag="RH058_001718"
     CDS             23372..23839
                     /gene="ybeY"
                     /locus_tag="RH058_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV3137310.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            23929..24807
                     /gene="corC"
                     /locus_tag="RH058_001719"
     CDS             23929..24807
                     /gene="corC"
                     /locus_tag="RH058_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV3137311.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            24832..26370
                     /gene="lnt"
                     /locus_tag="RH058_001720"
     CDS             24832..26370
                     /gene="lnt"
                     /locus_tag="RH058_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV3137312.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(26448..28325)
                     /locus_tag="RH058_001721"
     CDS             complement(26448..28325)
                     /locus_tag="RH058_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDV3137313.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLFSSVFLHSSFSHLLMNMFALLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAALATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            28780..29688
                     /gene="gltI"
                     /locus_tag="RH058_001722"
     CDS             28780..29688
                     /gene="gltI"
                     /locus_tag="RH058_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV3137314.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            29858..30598
                     /gene="gltJ"
                     /locus_tag="RH058_001723"
     CDS             29858..30598
                     /gene="gltJ"
                     /locus_tag="RH058_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV3137315.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            30598..31272
                     /gene="gltK"
                     /locus_tag="RH058_001724"
     CDS             30598..31272
                     /gene="gltK"
                     /locus_tag="RH058_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV3137316.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31272..31997
                     /gene="gltL"
                     /locus_tag="RH058_001725"
     CDS             31272..31997
                     /gene="gltL"
                     /locus_tag="RH058_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV3137317.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32115..33050
                     /gene="rihA"
                     /locus_tag="RH058_001726"
     CDS             32115..33050
                     /gene="rihA"
                     /locus_tag="RH058_001726"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV3137318.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            33134..34804
                     /gene="hscC"
                     /locus_tag="RH058_001727"
     CDS             33134..34804
                     /gene="hscC"
                     /locus_tag="RH058_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDV3137319.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(34864..36315)
                     /gene="djlC"
                     /locus_tag="RH058_001728"
     CDS             complement(34864..36315)
                     /gene="djlC"
                     /locus_tag="RH058_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDV3137320.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLAELCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(36312..>37007)
                     /locus_tag="RH058_001729"
     CDS             complement(36312..>37007)
                     /locus_tag="RH058_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3137321.1"
                     /translation="EQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGIN
                     NRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTA
                     MCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMHYFSA
                     YSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQ
                     PECPVTLKDVSDL"
BASE COUNT         9078 a         9532 c         9320 g         9077 t
ORIGIN      
        1 gtggtaggcc tgtgcattat cggctggttc tggtttagcc cgacgcttta cgttgattcc
       61 tggaatccgc atcatgcacc gttcttcagt gcagtaggtt cttccatcgc tatgacgctg
      121 tgggcttttc tcggtctgga gtctgcgtgt gcgaatactg atgtagtgga aaacccggaa
      181 cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc
      241 tccaccaacg tgattgcagg gattgtgcct aatatggagc tggcaaattc aacggcacca
      301 tttggcctgg ccttcgcaca gatgttcaca ccagaagtgg gtaaagtcat tatggcgctg
      361 atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt
      421 aaatcttcat ctgatgaagg ctacttccct aaaattttct cccgtgtaac caaagtggat
      481 gcaccggtgc agggaatgtt gaccattgtg attatccaga gcgggttggc gctgatgacg
      541 attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt ggtgaccaat
      601 atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg
      661 cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtatagcttt
      721 tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt gaccttcctt
      781 ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa acacggttaa
      841 aaaagcaaag taaaaaaggg cgatcttgcg accgcccttt ttttattaaa tgtgtttacg
      901 cgtttttcag aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt
      961 cttcaccgag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct gacggacgcg
     1021 cggcgaacca gccgttgtcg gtcatcactt tcagaccgcc gatagaagca ccattgcccg
     1081 gtgccgccgt caggcgcgcg gtgatcgggt cgcctgcaag ggtgctggcg ctcaccattt
     1141 ccggagacag cttagacagc gccgcttttt gtgcggaagt cgcggctgcc tgcaaacggt
     1201 tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc gggttcttac
     1261 cggtgacagc ggtgatttcc gccgccagca gacacataat gatgccgtct ttgtcggtgg
     1321 accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc
     1381 cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa acctaccggg acttctacca
     1441 gcttacggcc caagtcattg accacacggt cgatcatcgc agaagaaacc agcgttttac
     1501 cgacagcaac atctttgccc cactgcggac gatgctggaa caggtagttg atcgccaccg
     1561 ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat
     1621 ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg
     1681 cacactcgga ggagcagtcc atacggatcg cgccgtcttt atcaaggtgc ataaagcgga
     1741 aggtttgatc gacctgatcg ttaacgatgg tcaggttgag gttgtaatac tcgccaatac
     1801 gcttccagta ttcgataccg gaaccgccca gcggatcaac gcccagcgtc aggcccgctt
     1861 tctgaattgc cgccatatca acgatatcgg ccagaccttc cacaaacggc tgcaccagat
     1921 cctgctcttt tacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt
     1981 tcaggccatc ggccatcagt gcgttggccc tgtcttccac cactttggtg acgttggtat
     2041 cagccgggcc accatttggc gggttgtact tgataccacc atcttccggc gggttatggg
     2101 acggtgtaat cacgataccg tctgccagcg ggccaccttt tttattgtga accaggatgg
     2161 cattggaaat ggcaggtgtc ggagtgaagc cattgttttc ctgcacaatg acatcaacgc
     2221 cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat
     2281 ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct
     2341 gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac
     2401 cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat
     2461 attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac
     2521 gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc
     2581 ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat
     2641 ttacggccgg tgttggtgtt ggtgatcacc caatacggcg tgcctggcac atgtttcggc
     2701 ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta
     2761 cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga atatagtgta
     2821 gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc atccgaaagc
     2881 agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg
     2941 atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg
     3001 gaaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg
     3061 ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc
     3121 agtgtcttag ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc
     3181 gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac
     3241 aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg
     3301 aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccat
     3361 ggtctgtttg gtagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac
     3421 aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat
     3481 tacccggcga tggctcagga tcttgttgat accctggatg cacagcagat cgacaaagca
     3541 acatttatcg gtcactccat gggcggtaaa gcggtaatgg cacttactgc actggccccc
     3601 gatcggattg acaagctggt ggcaattgat attgctccgg tcgattatca cgttcgccgg
     3661 catgatgaga ttttcgcggc catcaacgca gtcagtgaat cggacgcaca aactcgccag
     3721 caagcagcgg caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgctg
     3781 aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg tattgtggga tcagtatccg
     3841 catattgtgg ggtgggagaa aatcccggca tgggatcacc ctgccctgtt tatccctggc
     3901 ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca atttccacag
     3961 gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg
     4021 gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgt
     4081 ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc gcgctgatgt attatggcgc
     4141 gctatccatc cgggcagagg cctggctgat tgtttccccc gaagtcacca agatcatggc
     4201 caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg
     4261 accgaaaact aatccgcttt cgcgcgatga acagctgcgt attaataaac gcaaccagct
     4321 aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg cggaagctgt
     4381 cgaggcgctg aacgagctgg cggaatcgcg caatatgagc cgtagcgaac tgatcgaaga
     4441 gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccagttt
     4501 catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct
     4561 atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta
     4621 tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata
     4681 tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa
     4741 aaagcagcaa agaagatctg gaagcttatg acattctgct gctgggcatc ccaacctggt
     4801 attatggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt
     4861 tcaatggcaa actggttgcg ctgtttggtt gtggtgacca ggaagattac gccgaatatt
     4921 tctgcgatgc attgggcacc atccgcgaca tcattgaacc gcgcggtgca accatcgttg
     4981 gtcactggcc aactgcgggc tatcatttcg aagcatcaaa aggtctggca gatgacgacc
     5041 actttgtcgg tctggctatc gacgaagacc gtcagccgga actgaccgct gaacgtgtag
     5101 aaaaatgggt taaacagatt tctgaagagt tgcatctcga cgaaattctc aatgcctgat
     5161 gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt
     5221 gctacaaatt tgtaactttt gctgttgtac ctgtacaatg tcccggtgtt caagtggcct
     5281 tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgtg
     5341 gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag caaattctgt
     5401 cacttcttct aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca
     5461 ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag
     5521 ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg
     5581 atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgacg
     5641 ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc
     5701 aacatcacca cgatcacctg atctgcctcg actgcggcaa ggttatcgaa tttagtgatg
     5761 attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc
     5821 acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat gaacatgcgc
     5881 acgaaggcaa ataagccagc ctgaatgaga aaaagccaac ctgcgggttg gcttttttat
     5941 gcaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt
     6001 tcctgccaaa ctttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca
     6061 cgcagacact gctgataatc cagtacgcgt acactttcct ggtcggcaaa ctgctgatgt
     6121 tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttctggcgaa
     6181 ccttgcgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc ttcctgtggt
     6241 gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag aatcagtttt
     6301 ttcattgaaa attcctcaaa cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa
     6361 gatggtgaat ggtgcgatta ccataaattt cacgtcacgc tcatcctgga agatgttacc
     6421 gtaaccgccg ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt
     6481 gaacatcgtg cctttggcgc gaccgtcctg aatggtgtag accgcatcca ggctgtatgc
     6541 agactcttca atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc
     6601 aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca
     6661 gtttttcagg tcatacatcg caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc
     6721 ggaacggtta tcccaccaga tatccaggcg gccgttggag gaagcgtagg ttggagtcat
     6781 acgttgcagg aagtatccct gctgaccgtc agccttaacc caggtgcctt cgaggcgcaa
     6841 atccactacg tcagccgccc ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc
     6901 ataaaggtcg ttgacgctgc gatcgtcaac tttatcgcgc gtaccataga actggtagct
     6961 ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa aatattgatc
     7021 gatataccct tccgcctgac caaatgccgc ttccagtacg aagttatttt tgaagtcgta
     7081 tttcgcccca agggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc
     7141 atccatttcc agatgccacg gcgctttgta ttcgttggtc cacatgtagg agaaactcaa
     7201 ggcaccagca tcgccgtaat caaaattcgc cccggcttcc gccccctgat aagtacctgg
     7261 cataaagctc cagtgcggcg ctaacagcgt ttgaccagtt ggctgaatgt aacctgccct
     7321 cgcccaaacc ggaccgtatt taaatttggc cgcagcttta tacaggctta taccgctttt
     7381 gtcgccggac cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg
     7441 gtgggagctg tcgccgtttt ccgccatttc aatcgccgta aacgcagcaa tatcaaggcc
     7501 gaacatatca gcagcataac cggactgaaa atcgaggttg gcattccagg tggagtgaga
     7561 aaggttggtt ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca
     7621 gtaatatata ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg
     7681 cattgccata aagcccgaca ttgctgtaac accggcgata gccagcgcca gcgtactacg
     7741 tttgccacta aacgtacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag
     7801 gtgaacagcg aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca
     7861 ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga
     7921 acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa
     7981 aggcttgcag aagaataaaa gcgtgaattt ccagcaagat gaaagcggtt acattttatt
     8041 taattatggt ttgtgatttt aaatttattt tatggataaa ttgcgaagcg gaattaattc
     8101 gcgaagaaaa gcagaaaaaa cgccgcagag tgcggcgttt gggattgttt gctatttctt
     8161 gccggatgcg acgtaaatgc ctcacccggc ctggtagaac ttaaaattac tcgcctactt
     8221 tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gctgtggaat
     8281 ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgcaaccg
     8341 catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca
     8401 ccgacaggaa atcatccgca gcaccagggt tcggcacgct gaacagacgg tcgtacagac
     8461 ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct ttgactttac
     8521 gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga
     8581 tattgccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca ttacgcagac
     8641 gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg
     8701 cgcgatcgat ccaaatctca ccgctaaacg gcacctgacg actccccatt tccggtttgt
     8761 tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg ataaccagtt
     8821 tcaccggatc gataaccgcc attgcgcgcg gcgcattttc gttgagatct tcacggatgc
     8881 aggattccag cgatgccatc tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc
     8941 agaactcacg aatagaagca gcagtgtaac cacgacgacg cagaccggaa atggtcggca
     9001 tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct
     9061 tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag
     9121 gaatggtgat gttgtccagc acccagtcgt acaggcgacg gttgtcctgg aactcaagcg
     9181 tacacagaga gtgcgtaata ccttccaggg catcgctgat gcagtgggtg aagtcgtaca
     9241 tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta atacggtaca
     9301 gcaccggatc gcgcatcacg ataaacggtg aagccatgtc gattttcgca cgcaggcagg
     9361 ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa
     9421 cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt cagggtgccg cggtattcgc
     9481 ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg
     9541 cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa
     9601 aacctaacca ctctacgtcg tttttgatcg actcaacata ctcgatatct tcttttaccg
     9661 ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt
     9721 tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac
     9781 gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa
     9841 agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta
     9901 taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt taaaattgat
     9961 aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga agatctccac
    10021 cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca ggcataaagc agattacttt
    10081 ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt aatgatcaag
    10141 ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg
    10201 ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg cgcaccctct
    10261 tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc catatggacg
    10321 acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt gaagattttc
    10381 acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac cgccacaccg
    10441 tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc cacgacatca
    10501 ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc ctgcggtttt
    10561 gctacaggcg cggcagttgc cggagttgct tcagctgacg cagctgctac cggaccgcgg
    10621 gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac aatcacctga
    10681 atagtctgtt tgttcagttt tactacacca gaagcaccca gacgtttaca catcgtatcg
    10741 ttaacgcggg cagagtcagc cactgtaagg cgcagacggg tgatacaggc gtcaattgct
    10801 ttcaggttgt cagtgccgcc aaccgcagca atatagttgg tcgccagttg attgaggcct
    10861 tcttcagtgt tgctgttggc ttcttcagta acgatctcgt cttctttatc ttcacgaccc
    10921 ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta gatagcgaag
    10981 aagacaacac ccatcaccag cagcatccag acgttctggc tggcggccgg caggttatac
    11041 atcaacgcgt agtcgatagc ccccgcagag aaagagaagc cagcatggat acccagcagt
    11101 gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag cagcggtgca
    11161 aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc agtaacagca
    11221 acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc gaagtacatc
    11281 gccagcgccg cacccggcag accgaacatc ataatcggga agaagccgga catgaacatc
    11341 cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac cgtggaaaac cgtacccgcc
    11401 gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg atgcagaccg
    11461 gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc gcccgcagaa
    11521 acgatccact cgcctcctgc atggatagcg tgctgtaccg gcggccagac gtaaccaaaa
    11581 atggccgcca gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg
    11641 aagaaactca ggaagtccgg cagtttaata tcggaccaac ggttataggc tgcgccacca
    11701 accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt gatggtcacc
    11761 atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc accggcgctg
    11821 tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt atcaaaaatc
    11881 gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc
    11941 aacagtgccg ccaccggcag caccgcgata gggagctgta acgccctacc gagtcgctgg
    12001 aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc tcgtttaaag
    12061 ccgtattttt attttgctgc aaattgtact gccgatgttc tgtaatcaga ttgttagatc
    12121 atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt
    12181 gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac cagaaaactt
    12241 attttatcat tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc
    12301 ttaaagatgc ctaatccgcc aacggcttac attttactta ttgaggtgaa taatgagact
    12361 gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata tcgtcaatcg
    12421 tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgtt ctgggcctgc cgactggcgg
    12481 cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt
    12541 taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag agcatccgga
    12601 aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc cagcagaaaa
    12661 catcaacctt ctcaacggca acgccccgga tatcgacgcc gaatgccgcc agtatgaaga
    12721 aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta acgacggtca
    12781 tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca aaaccttgac
    12841 tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc aggtgccaaa
    12901 atatgccctg actgtcggtg ttggtacatt gctggatgcc gaagaagtga tgattctggt
    12961 gctgggtagc cagaaagcac tggcactgca ggctgcagtt gaaggttgcg tgaaccacat
    13021 gtggaccatc agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc
    13081 caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag cagaaaatat
    13141 caaaggtctg taattgttat ccctgccctg gctccttgct cagggcaata tttttaaaaa
    13201 tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca cgaatttctt
    13261 gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa
    13321 ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc cggttttatc
    13381 gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga agcggtcagc
    13441 gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac taactatctg
    13501 ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag
    13561 tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat
    13621 ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc cgcgctggtc
    13681 gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc ggaaatggtt
    13741 cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc cggtcactcc
    13801 aacgcgacgt tgaaagaagc gaaagccggt ttccgcgcgg ggattacctt tgccacccat
    13861 ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc
    13921 gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga ttacgccaac
    13981 attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga cgccaccgcg
    14041 ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaac
    14101 ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc
    14161 gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg tatggcgacg
    14221 ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc cgcaggtaaa
    14281 gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat cgttaacggt
    14341 aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct cagataggta
    14401 atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt gaccagtacg
    14461 ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc agcgtaacca
    14521 aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag caggcctcca
    14581 ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac gcaatcggcg
    14641 tacggcttgg tcgtcacgac gccaccatca ccctgtttga tctcagcagc aaagtgctgg
    14701 cagaagaaca ttacccgctg ccggaacgta cccagcaaac gctggaacat gccctgttga
    14761 atgccattgc tcagtttatt gatagctacc agcgcaaact gcgcgagctg atcgcgattt
    14821 cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac atgccgcata
    14881 ttcaggtaga aaactggggg ctggtagaag cactggaaga acgttttaaa gtgacctgtt
    14941 tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt gcaagtcagg
    15001 attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg attatctcta
    15061 acgggcgcat ttttattggc cgcaacggca acgtcggtga aattggccat attcaggtcg
    15121 aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact atcgctgcca
    15181 acgccgctat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgcgtgc
    15241 cgctggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagcctgg
    15301 cctcggaagt gattgagtat gtcggtcgtc atctgggcaa aaccatcgcc attgctatca
    15361 acttatttaa tccgcaaaaa attgttattg ccggtgaaat caccgaagcc gataaagtgc
    15421 tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt cgcactaatc
    15481 tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt gcgctggtaa
    15541 aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta
    15601 tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg
    15661 cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt
    15721 gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac
    15781 cgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt
    15841 ttatacctct gcgatggcga ctgccgattt cctgcgtcgc caggaaggca agaaagcgta
    15901 tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga
    15961 tgtgaaccct gattttgtga ttgttggcga aacgcgttcc tacaactggg acatgatgca
    16021 taaagcggcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacaccca
    16081 cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg
    16141 gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg cattaaacaa
    16201 aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacttgcgta ccgatattct
    16261 ggcgggcttc caggcgggtc tggagacgat tctggtgctt tctggtgttt cgtcgctcga
    16321 cgatatcgac agtatgccgt tccgcccaag ttggatttac ccatcggtcg ctgaaatcga
    16381 cgttatctga atagcaacca cgtctgcgga cgtggtttct tatttttacc catcattaaa
    16441 atcatcccat tcaataaaac ccgtcaaaaa ttatcgatcc atcaataaat acgccaccac
    16501 accctatcct ttttcacaaa tcagcaatca ccattacgtt ttttattttt tcaccgccaa
    16561 atcgcttgcg cacagtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
    16621 caaataccaa gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
    16681 cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg
    16741 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgctcac gaacgtctgt
    16801 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac
    16861 tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat ggcgatcgtt
    16921 atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc
    16981 cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag
    17041 atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg ggctatgacg
    17101 aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt tgccgcacga
    17161 ttaaagagtt cccggcgggg agctatttgt ggagccagga cggcgaaatc cgttcttatt
    17221 atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc
    17281 tgcgtcaggc tctggaagat tccgttaaaa gccatctgat gtctgatgtg ccttacggtg
    17341 tgctgctttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag aaatacgcag
    17401 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcagtta cactcctttg
    17461 ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca aaccatctgg
    17521 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
    17581 tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca ccgatgtatt
    17641 taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg
    17701 atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa gaactgcatg
    17761 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag
    17821 cgatgtccgc ctggggcgtg gaagcgcgcg ttccgttcct cgacaaaaaa ttccttgatg
    17881 tggcgatgcg tattaaccca caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca
    17941 tcctgcgtga atgttttgag tcatacctgc ctgcaagcgt ggcctggcgg cagaaagagc
    18001 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc
    18061 aggtttctga tcaacagctg gaaactgccc gcttccgctt cccgtacaac acgccgacgt
    18121 ccaaagaagc gtatctgtac cgggagatct ttgaagaact gttcccactt ccgagcgccg
    18181 ctgagtgcgt accgggcggt ccttccgtcg cgtgttcttc cgctaaagcg attgagtggg
    18241 atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg
    18301 cgtataagta agaaatgcga aacagccccg gtgaaatccg gggctgtttc agttattgag
    18361 gtgcttgtta attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    18421 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttgaatt
    18481 ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa
    18541 acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga
    18601 gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc
    18661 aacgccgata aggtatcgcg aaaaaaaaga tggctacgta gctcagttgg ttagagcaca
    18721 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccatc ttttttgcgg
    18781 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    18841 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    18901 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    18961 cttcttcgag taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc
    19021 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa
    19081 tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    19141 gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa attcaaaaac
    19201 caatttgttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg cattccgagg
    19261 ttcgaatcct cgtaccccag ccacattaaa aaagctcgct tcggcgagct ttttgctttt
    19321 ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt atccttcaat gtcggatgcg
    19381 acgctgccgc gtcttatccg acctacggtt ggcacgcatc cggcaatgtt gtaaggctac
    19441 aaccctaacg catatttcag cgcctgacgt ttcaacacgc cagcacgctc cgccgccatc
    19501 aaaccaagat tacgcacaaa acgcagcggc ggcagattat tactgaatcc ggcataaaac
    19561 agatccatac cactttgcat aatgaagtta tccgccatgc gccgcatctg gtaacgcttg
    19621 aggacaggat aactggccca cgcttcaccg tagctgcggg cattcaccag aacatcaatc
    19681 agggcatcga catcacgata accaagattc accccctgcc ccgccagcgg atggatggta
    19741 tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat actgcaacgc atgtcggcgc
    19801 gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac ccagacgcga cgggaaatgc
    19861 ttcgcgattt ccgcctggag ttgcgccata ttcatattct gcaactggcg aatacgcacc
    19921 ggagagtcat accacaccag cgatgcccag ttatcaaaca acggcagaaa cgcacgcggt
    19981 ccgtccgggg taaattgctg ccaggtgctg tcgccgggat cgttctcaca ctgtacgcta
    20041 atcaacatac acgactgcgc atactgccag gcatgaacgc caattcccgc catctgccgc
    20101 acctgcgaat ttgcgccgtc agcaccaatc accagcttcg cgcgaatcac ttcaccgccc
    20161 ttcagttcca gctcctgaag atcattatct cgatgtaatg caatcagcga gcctggcaca
    20221 cgtaacgtta ctttcggatg agcttccagc gcctgccaca atgcctgttg caggacggtg
    20281 ttttccacca tatagccaag cagcggcagc ttaagttcag cggcgtcaaa catcacatgc
    20341 gccgtttccc actcccacgt ttccagtctg cggtaaggat ggcaacgcat agcctgtact
    20401 gcatcccaga cccctaaccc tttaagcaat gataccgatg cggcgctgat cgccgagatc
    20461 cgcacgtccg gttggctatc agcgacaaac ggtgcaggtt ctgcgtgctc gatcaccgtt
    20521 accgaaaatc cgtgctgtgc cagccccagc gccagtgcgc cgccgaccat tcctccgccg
    20581 acaatggcaa tttccgttgg ttgatttgtc atggtcaatc atcctgttag caatatgctt
    20641 taagtttaca ggatttttac ccccaagggc tgataacgct catactggtc acaacggcag
    20701 caaagcatta cactatgcgc cctgcattcc tggctactat ttcgcaagag caagtcgatg
    20761 accaaaaaac tccatattaa aacctggggc tgtcagatga acgagtacga ttcatcgaag
    20821 atggccgatc tgctggatgc cacccacggc tatcaactga ccgacgtggc ggaagaagcg
    20881 gatgtgctgc tgctgaacac ctgctcaatc cgcgagaagg ctcaggaaaa agtcttccat
    20941 cagttgggtc gctggaaact gttaaaagag aagaatccag acctgattat cggcgtcggt
    21001 ggctgcgtgg catcgcaaga aggcgagcac attcgccagc gcgcccacta tgtcgatatt
    21061 atttttgggc cgcaaacgct gcaccgtctg ccggagatga tcaactccgt gcgcggcgac
    21121 cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga agtttgaccg tctaccggaa
    21181 ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg aaggctgcaa taaatattgt
    21241 acctactgcg tggtgcctta cacccgtggt gaagaggtaa gccgtccgtc tgacgatatt
    21301 ctgtttgaga ttgcccagct tgctgctcag ggcgtgcgtg aagtcaacct gctcggtcag
    21361 aacgtgaacg cctggcgtgg tgaaaactac gacggcacca ccggatcgtt tgccgatctg
    21421 ctgcatctgg ttgctgcgat cgacgggatc gatcgtattc gctttaccac cagccatccg
    21481 atcgaattca ccgacgatat catcgaagtg taccgcgaca cgcctgagct ggtgagcttc
    21541 ctgcatctgc cggtacaaag cggttccgat cgcattctga acctgatggg ccgtactcac
    21601 acggcgctgg agtacaaagc gatcatccgt aaactgcgtg cggcgcgtcc ggatattcag
    21661 atcagttctg acttcattgt tggcttccct ggcgaaacca ccgaagactt cgagaaaacg
    21721 atgaagctga ttgccgacgt caatttcgac atgagctaca gctttatctt ctctgcacgt
    21781 ccggggacgc ccgccgccga tatggtggat gatgttccgg aagaagagaa gaagcagcgt
    21841 ctgtatattc tgcaagagcg cattaatcag caagcgatgg cgtggagccg ccggatgctc
    21901 ggcaccaccc agcgtattct ggtagaaggg acttcgcgta agagcatcat ggagctttcc
    21961 gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca caccggatat gatcggtaaa
    22021 ttcgtcgatg tagaaattac cgacgtctac ccgaactctc tgcgcggtaa agtggtgcgt
    22081 actgaagatg aaatgggtct gcgcgtggca gaaacaccgg aatcggtgat tgcccgtacc
    22141 cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt aattctcagg cccgccgttc
    22201 cggtgggcct tgttttttct ctctttatcc ccatctttat cgcaatgctt gcgccttttt
    22261 cctgtggcgt gaataatttc cttataggcc ggtgaatgtt cagcttccgg cgacatacag
    22321 ataagaggaa cggtttgaac atagacactc gcgaaatcac cctggagcca gcagacaacg
    22381 cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat caagcagctc gaacgccgtc
    22441 tcggcatcga gatcaatcgc cgcgacaacc actttaaact gaccggccgt ccgatttgcg
    22501 tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga tacagccccg atgcgcggtc
    22561 agattcagga tatcgaaccg gaacagatcc accttgcgat taaagaagcg cgagtactgg
    22621 agcaaagtgc ggagagcgtg ccggagtacg gcaaagcggt caatatcaaa accaaacgcg
    22681 gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat cgccaatatt ctcgaccatg
    22741 acattacctt cggcgttggc ccggcgggta cgggtaaaac ctacctggca gtcgctgcag
    22801 cagttgatgc cctggagcgt caggaaattc gccgtattct gctgactcgt ccggcggtcg
    22861 aagccggtga gaaactgggc ttcctgcctg gcgatttaag ccagaaagta gacccgtatc
    22921 tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt tgagaaagtt gagaaactga
    22981 ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat gcgtggtcgt acgctgaacg
    23041 acgcgtttat cattctcgat gagagccaga acaccaccat cgaacagatg aagatgttcc
    23101 tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg cgacgtcaca cagatcgacc
    23161 tgccgcgtaa tactaaatca ggcttacgtc acgccatcga agtgctggcc gatgtcgaag
    23221 agatcagctt taacttcttc cacagcgaag acgtggttcg tcaccctgtg gtggcgcgta
    23281 tcgttaacgc ctatgaagcc tgggaagaag ccgaacaaaa acgaaaagcg gcgctggcgg
    23341 cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag gtgatcctcg atttacaact
    23401 ggcatgtgaa gataattccg ggttaccgga agagagccag tttcaaacat ggctgaatgc
    23461 ggtgatcccg cagtttcagg aagaatcgga agtgactatt cgcgtggtcg ataccgccga
    23521 aagccacagt ctgaatctga cctatcgcgg taaggataag ccgaccaacg tgctctcctt
    23581 cccgtttgaa gtgccgcctg gcatggaaat gtcgctactg ggcgatctgg ttatctgccg
    23641 tcaggtggtt gagaaggaag ctcaggagca aggcaaacca ctggaggcgc actgggcgca
    23701 tatggtggtg cacggcagtc tgcatttgtt aggttacgat cacatcgaag atgacgaagc
    23761 agaagaaatg gaagccctcg aaacagagat tatgcttgct ctgggctatg aggatccgta
    23821 cattgccgag aaagaataat ttgccaggca tttgactggc agacgtcccc gccgttggtg
    23881 attaacgacg gcgttttgat taactaacat gagaacccat acgacgccat gagcgacgac
    23941 aattcacaca gtagtgacac gataagcaac aagaagggat ttttctccct gttactcagc
    24001 caacttttcc acggtgaacc gaaaaaccgt gacgaactgc tggcgctgat ccgtgattcc
    24061 gggcagaacg accttatcga cgaagatacg cgcgatatgc tcgaaggggt gatggacatc
    24121 gcagaccaac gcgttcgcga catcatgatc ccccgctccc agatgattac cctgaaacgc
    24181 aaccagacgc tggacgaatg ccttgatgtc atcatcgagt ccgcccactc acgtttcccg
    24241 gtgattagcg aagacaaaga tcacattgaa gggattctga tggcgaaaga cttgctgccg
    24301 tttatgcgca gcgatgctga agccttcagc atggacaaag tgttacgtca ggcggttgtc
    24361 gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt ttcgctctca gcgttaccac
    24421 atggcgatcg ttattgacga attcggtggg gtttccggtc tggtgaccat tgaagacatc
    24481 ctggaactaa ttgttggtga gattgaagac gagtatgacg aagaagatga tatcgacttc
    24541 cgtcagctga gtcgtcatac ctggaccgtg cgcgcactgg cttccattga agacttcaac
    24601 gaagcgttcg gcacccactt tagcgatgaa gaggttgaca ctatcggtgg tctggtgatg
    24661 caggcatttg ggcatcttcc ggcgcgtggc gaaactatcg acatcgacgg ttaccagttc
    24721 aaagtggcga tggccgacag tcggcgtatt attcaggttc atgtcaaaat cccggatgac
    24781 tcaccccagc cgaagctgga tgaataaaac cgaaactgga tagataacta catggctttt
    24841 gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg cgttattatt cggtgcctgc
    24901 ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg cgattatttc gttgatgggg
    24961 cttcaggcgt tgacctttaa ccgccgtcca ctccagtctg ccgctattgg cttttgctgg
    25021 ggatttggcc tctttggcag cggtattaac tgggtctatg tcagcatcgc gacctttggc
    25081 gggatgcctg gcccggttaa catcttcctg gtggtgctgc tggcggcgta tttgtcgctg
    25141 tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc cgaaaaccac ctggctgcgc
    25201 gtggcgattg ccgccccttc tctctggcaa gtgaccgagt ttctgcgcgg ttgggtactg
    25261 acaggcttcc cgtggttaca gttcggctat agccagattg atggcccgtt aaaagggctg
    25321 gcaccgataa tgggcgtgga agccattaac ttcctgctga tgatggttag tggcctgctg
    25381 gcactggcgt tggtcaaacg caactggcgt ccgctggtgg tggccgtcgt gctgtttgcc
    25441 ctacccttcc cgctgcgtta catccaatgg tttaccccac aaccggagaa aaccattcag
    25501 gtttcgatgg ttcagggcga tattccgcaa tcactgaaat gggatggaga tcagcttctt
    25561 aatacgctga agatttacta caacgcaacg gctccgctga tgggcaaatc atcgttgatt
    25621 atctggccgg agtcggcgat aaccgatctg gaaattaatc agcaaccgtt cctcaaagca
    25681 ctggacggtg agttgcgcga taaaggtagc tcgctcgtaa ccgggattgt cgacgcgcgt
    25741 ctcaataagc agaaccgcta cgatacctac aacaccatca tcacactggg taaaggtgca
    25801 ccgtacagct acgaatcagc cgatcgctat aacaaaaacc atctggtgcc gtttggcgag
    25861 tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt tctttgatct gccgatgtcg
    25921 tcgttcagcc gtgggccata tatccagccg ccgctgtcgg taaatggtat tgagcttact
    25981 gcggccattt gctacgagat cattcttggc gagcaagtgc gcgataactt ccgcccggat
    26041 accgactatc tgctgactat ctccaacgat gcgtggtttg gtaaatctat tggtccatgg
    26101 caacacttcc agatggcgcg aatgcgtgcg ctggagctgg cgcgcccact gttgcgcagc
    26161 accaacaacg gcattacggc ggtgattggc ccgcagggtg agattcaggc gatgatcccg
    26221 cagttcaccc gcgaggtgtt aaccactaat gtgacgccga ccaccggact cacaccatac
    26281 gcacgtaccg gcaactggcc gctgtgggtg ctgacggcac tgtttggttt tgccgctgtg
    26341 ctgatgagtc tgcgagcacg taaacgttga tgtgcctgat gtatcctgca tgagcaaaac
    26401 gtactttctc attgagcttt ggggcatggt tatcatgccc ctttttctta cggttcaggg
    26461 atacaagcca cattgccata ttggggatga cgaataattg cctgcggatc aagcgagcgc
    26521 gtcctgtcca gttggatcaa cccgttcttc gccataacaa atatttttct accatccgct
    26581 gaaaaacgaa ggtgattaac gtagctggca ggataaacgc caataatctg cccggcatta
    26641 atattgagca ggatgacata accttgcagg ccatatgaca ccaacgctaa atactgaccg
    26701 tccgggctaa taccctcaac ggaataaacg ctcctgctac accattgcgt cataatgctg
    26761 gcttgttgat cccctacgct tatacgtgaa atcccctggc tgtcggcaaa aattacatcc
    26821 ccgttttcag gtcgaaaacg catcgccgat atcgttcctc gcgcatcact agccggatag
    26881 ctttctttct gatgcgtgat ggtattgatg cgtacaatgt cacctctgac cataccgggt
    26941 atcaacaata ttttttcatc cggggaaaga gccataaccc aggcaccagg tttattactc
    27001 gtccccgtcc cgtcgtattt ctccagcggg atatccgcca catgctgata agttaccaga
    27061 tcaatcgctg acacactatt actggtgcga ttcatcaccc aggcttttgt accatcacgc
    27121 gagaggataa ggctgtcagg atgtgcaccg gtggtaatcg attgaataat ctcccctgtc
    27181 gccacgttaa tgaccgataa cgcattcgct tcaaatgaag tcgcataaat caacttttca
    27241 tccgggctta atgccagcga tctgacgccc tgatctttac agccaccaca atgtttgact
    27301 gacttttctt tcgctatggg cgctgtaaag gtatgcagga ttttcttttg tccgaggtcg
    27361 aaaacaacaa tgctgttcgt gttttcaatt gcggcataca acatattacc atcccgactg
    27421 atcgccatat catggatatc ggcaatctca gcgagcaaat caccactggc ctgttcacga
    27481 cttaccggag cggataatgt ttccctgagg gcgttgcgtt cctccgctaa ctgctgcttt
    27541 ttttgccgtc gctcttgttc aatctcctgt ggataaaacg cttcacgcag gctttgcctg
    27601 acttgtaaaa ctgactcatc catctgctgc tgcgcaagcc agatagcccc ggtcagaaga
    27661 agactgcccg caacaataat cccgccttcc gtaacgagtc ggttttgccc agataaacgc
    27721 gcgctcagcc aacccaacgc gccacccgca atcaggccgc caatgtggca agcattatct
    27781 attcctgctt gccgggcacc atacaacagt gtcagcgcca ccatacccaa cagagaaaat
    27841 actcgccgct ggtttttatg gtatgtgccc gcacctgaag caagctgtgt tgctaacgca
    27901 gcgccagcta ttcccataat tgccccggac gccccaacgc tgatgactat ctgatcacta
    27961 tcgcgtaacg cataacaggc gctgatgagc ccaccaaaga cgccggagaa taaccaaata
    28021 atcagaagtc gccattttcc cagtatccgt tccgccactg cccccaccac caggagtgca
    28081 aacatattca tcagcaaatg ggaaaaacta ctgtgcagaa agacactgct aaataagcgc
    28141 caggattcac ccgacaacgt gagagaagca acgttagcac cgagatggat gagtgcggat
    28201 tgatcgactt cttcataact catccattgt gcgaggtaac aaaatatccc aacacagagc
    28261 gcaaaaagga taagggttat tgcgggtaat tgaacattta acggcttaac cgaagatgca
    28321 gacataaggt gtttcttcct tgaaaagaca ggtaaaaagc atttttcccg tgtacagtta
    28381 tcatcccggc aaacgtccca tgccgaaaag ataactctgt ccgaaatatg actttttcag
    28441 cctgaaatta caatcgtaat taattcaccc ctaagtattt tttggcatgt aagttgcttt
    28501 atttactgtg taagcaatga aatcagccgc gacgcaaccg ggttgcacca ccacaaatca
    28561 gcggtagcac caaaagagtg cattttaaga attttgcttc ggaatggtgc ggtgcacctc
    28621 gcaaaaacaa acaataaaac atcaatatct ttacatttag ccaaactaaa tgttacctaa
    28681 caagcacaac actgcacaat aaagttgcag acgataacaa cacaaacact cacaacgggt
    28741 atccatgcgt tcttaacgca gaagataaag gagttggata tgcaattacg taaacctgcc
    28801 acagcaatcc tcgccctggc gctttccgca ggactggcac aggcagatga cgccgccccg
    28861 gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt
    28921 gaatcttcag tacctttctc ttattacgac aatcagcaaa aagtggtggg ttattcgcag
    28981 gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag
    29041 gtaaaactga ttccgattac ctcacaaaac cgtattccac tgctgcaaaa cggcactttc
    29101 gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc ggctttctct
    29161 gacactattt tcgtggtcgg tacgcgcctg ttgaccaaaa agggcggcga tatcaaagat
    29221 tttgccgacc tgaaaggcaa agccgtagtc gtcacttccg gcactacctc tgaagttttg
    29281 ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac
    29341 ggtgactctt tccgcaccct ggaaagcggt cgtgccgttg cctttatgat ggatgacgct
    29401 ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggatat cgtcggcaag
    29461 ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag atgatccgca gttcaaaaag
    29521 ctgatggatg acactatcgc tcaggtgcag acttccggtg aagcggaaaa atggtttgat
    29581 aaatggttca aaaatccaat tccgccgaaa aacctgaaca tgaatttcga actgtcagac
    29641 gaaatgaaag cactgttcaa agaaccgaat gacaaagcac tgaactaatt acaagaacca
    29701 ggggcggaaa ttccagccct ctcgattgtt acgtagcacg gacagactat acgcctgatg
    29761 gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc gagggtagca
    29821 gttaacgctg ctaccctctt ttttctggag tagatttatg tctatagact ggaactgggg
    29881 tattttttta caacaagccc cgttcggcaa caccacctat cttggttgga tctggagtgg
    29941 ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt atcgctttcc tcgtcggttc
    30001 atttttcggc attttacgta ccgttccaaa ccgttttctc tccggtctgg gtacgttgta
    30061 tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc tttacctggt atctggtgat
    30121 cccggagttg ctgccagaga aaatcggtat gtggtttaag gccgagctgg atcccaatat
    30181 tcagtttttc ctttcatcca tgctctgcct ggggctgttt accgccgccc gtgtttgcga
    30241 acaggtacgc gccgccattc agtcgttgcc gcgtgggcaa aaaaatgccg cgctggcgat
    30301 gggcctgacg ctaccgcagg cttatcgata cgtactgctg cctaatgctt atcgcgttat
    30361 cgtcccgccg atgacttccg agatgatgaa cctggtgaaa aactccgcca tcgcctcgac
    30421 tattggtctg gtggatatgg cggcgcaggc gggtaaattg cttgattact cagcccacgc
    30481 ctgggagtcg tttaccgcta ttacgctggc gtatgtgttg attaacgcgg ttatcatgct
    30541 ggtaatgacg ctggttgaac gtaaagttcg cctgcctggc aacatggggg gcaaataatg
    30601 tacgagtttg actggagttc cattgtccct tccctgccat atctgctcga cgggctggtg
    30661 atcacgctga aaatcaccgt cacggcagtc gtgattggta ttttgtgggg caccatgctg
    30721 gcggtgatgc gcttgtccag ctttgcgccc gtagcgtggt ttgccaaagc ctacgttaac
    30781 gtattccgct cgattccttt agttatggtt ttgctgtggt tttacctgat cgtgccaggt
    30841 tttctgcaaa acgtgctggg attatcacca aaaaatgata tccgtttaat ttcggcgatg
    30901 gtggcgtttt caatgtttga agcggcctac tattcagaga ttatccgtgc tggtattcaa
    30961 agtatttctc gtggtcagtc gagcgccgcg ctggcgttgg gaatgactca ctggcagtcg
    31021 atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg tgccgcttct gctcactcag
    31081 ggcatcgtac tgttccagga tacctcactg gtgtatgtgt tatctctggc cgatttcttc
    31141 cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg ttgagatgat cctatttgcc
    31201 ggatttgttt atttcgttat tagtcttagc gcgtcgttgt tggtcagcta cttgaaaaga
    31261 aggacagcat aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac tttcaggtgc
    31321 tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc ggcccgtctg
    31381 gttccggcaa atcaacgctg attaaaaccg tcaacggcct cgaaccagtg cagcaaggtg
    31441 aaatcaccgt cgatggtatc gtggttaatg acaagaaaac cgatctggca aagctgcgtt
    31501 cccgcgtcgg gatggtgttc cagcattttg agttgttccc ccatttgtcg attattgaaa
    31561 acctgactct ggcgcaggtg aaagtgctta aacgcgataa agcgcccgct cgtgaaaaag
    31621 cattgaaatt gctggagcgc gtgggacttt ccgcacatgc caataaattt ccggcgcagc
    31681 tttccggcgg tcagcagcag cgtgtggcaa tcgctcgcgc gttgtgtatg gatcctattg
    31741 cgatgctgtt cgacgaaccg acatcggcgc tggatccgga gatgatcaac gaagtgctgg
    31801 acgtgatggt ggaactggcg aacgaaggaa tgaccatgat ggtggtgacc cacgaaatgg
    31861 gctttgcccg caaagtggcg aatcgggtga tctttatgga cgagggtaaa attgtcgaag
    31921 actcgccgaa agacgctttc ttcgatgatc cgaaatcgga ccgcgcgaaa gacttcctcg
    31981 cgaaaatcct gcattaatca ctctggcgcg catacccaag tgcgcgccat ctcacgcttt
    32041 atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa agaagcacac
    32101 aacaaggagc aacaatggca ctgccaattc tgttagattg cgacccaggt catgacgacg
    32161 ctatcgcaat agttctcgcc ctcgcctcac cagagcttga tgtcaaagca attacgtctt
    32221 ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg ctgaccttgc
    32281 ttaatcgcac cgatattccg gtagcaggcg gcgcggtaaa accgttaatg cgtgagttga
    32341 ttatcgcgga caatgtgcac ggcgaaagcg gtctggatgg cccggcatta ccggaaccga
    32401 cgttcgcacc gcaaaactgt acggcggtag agctgatggc gaaaacgctg cgcgaaagtg
    32461 cggaacctgt cacgattgtg tctaccggac cgcaaactaa cgttgccttg ctgctcaata
    32521 gccatccgga actgcatagc aaaattgccc gtatcgtgat tatgggcggc gcaatggggc
    32581 ttggtaactg gacgcctgcg gctgaattta atatttacgt tgacccggaa gcggcagaaa
    32641 ttgtcttcca gtcagggatc ccagtggtga tggccggtct ggatgttact cacaaagcac
    32701 aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg caaccctgtt tcaaccattg
    32761 ttgccgaact gctggatttc ttcctcgaat atcataaaga cgaaaaatgg ggctttgtcg
    32821 gtgcaccgct gcatgaccca tgcaccatcg cctggctgtt gaaacccgag ttgtttacca
    32881 ctgttgagcg ctgggttggc gtggaaacac aggggaaata tagccagggt atgacggttg
    32941 ttgattatta ttatctgact ggcaataaac cgaatgccac tgtaatggtc gatgttgatc
    33001 gtcagggctt tgttgattta ctggctgatc gtctgaaatt ttacgcttaa cacacgtcat
    33061 tgattaagat caccacgcgt tacctgggtg gtcttaatct gtttatctct ttttctttca
    33121 agatataaat gcaatggata atgcagaact cgccattggt atcgatctcg gcactaccaa
    33181 tagtttaatt gccgtctgga aagacggtgc cgcgcaatta attccaaata agttcggtga
    33241 atatttaaca ccatccataa ttagcatgga tgaaaataat catattttag tcggaaaacc
    33301 ggctgtatca cggcgtactt cgcatccgga taaaacggca gcgttattta aacgtgcaat
    33361 gggcagtaat accaactggc ggttaggcag cgacacattt aacgcgccag aactgtcctc
    33421 tttggtatta cgctcattaa aagaagatgc cgaagaattt ctgcaacgtc cgattaaaga
    33481 tgtggtgatc tccgttccgg cttatttcag cgatgaacaa cgcaagcata cccgtttagc
    33541 agcggagtta gccgggttaa atgcggtacg cttaattaat gaacccacag cagctgcgat
    33601 ggcgtatggc ctgcataccc aacaaaatac ccgttcgctg gtgtttgatc tcggtggcgg
    33661 cacgtttgac gttacggtgc ttgagtacgc cacgccggtg attgaagttc acgcctccgc
    33721 tggcgacaac tttcttggtg gcgaagattt tacccatatg ctggtcgatg aggttttaaa
    33781 acgcgcggat gtcgccagga ccacgcttaa cgagagtgaa ctggcagcct tgtacgcctg
    33841 tgtggaagcg gcaaaatgta gcaatcaatc gccattgcac attcgctggc agtaccagga
    33901 tgaaacgcgg gagtgcgaat tttacgagaa cgaactggaa gatttgtggt tgccgctgct
    33961 caatcgcttg cgagtgccga ttgaacaggc gttgcgcgat gcacgtctga agccgagtca
    34021 aatcgacagt ctggtgctgg ttggcggcgc gtcacaaatg ccgctggtgc agcgaatcgc
    34081 cgtgcgtctg tttggcaaat taccgtatca aagttacgac ccgagcacca ttgtcgcgct
    34141 gggcgcagca atccaggccg cctgccgctt acgcagtgaa gatattgaag aggtgatcct
    34201 cactgatatt tgcccttact cgttgggcgt tgaagttaac cgccagggca tttccggcat
    34261 tttctcgcca attattgaac gaaacaccac agtaccggtg tcgcgtgtag aaacttactc
    34321 gaccatgcac ccggaacagg attctattac ggttaacgtc tatcagggag aaaaccacaa
    34381 agttaaaaat aacattctgg ttgaatcctt cgatgtgccg ttgaagaaaa ccggggctta
    34441 tcagtcgatt gatattcgct ttagttatga tatcaacggg ttgcttgaag ttgacgtgct
    34501 tctggaagac ggcagcgtta agtccagagt gattaaccac agcccggtaa cattgagcgc
    34561 gcagcagatt gaagagagtc ggacgcggtt atccgcattg aaaatttatc cgcgcgatat
    34621 gctcatcaat cgcaccttta aagccaaact ggaagagttg tgggcgcggg cgctgggtga
    34681 cgagcgagaa gagattggcc gggtgatcac cgattttgat gcggcgctgc agtcaaacga
    34741 tatggcccgc gtcgatgagg tcaggcggcg ggcgagcgtt tatttagcca ttgagacctc
    34801 ataatcacgg ctgccggatg cggcgtaaat gctgcatccg gcatcggcgc aaaaaacaca
    34861 tcgtcaacta aacaacatcg cgattttgcc gagagcataa cctataacca gcaccgttaa
    34921 actgattttg ataatgctgg taatgctctg tggtttttca ccctcatcat ttttttcttc
    34981 atggttttca ggtgttggct catcgacatc ttcatcctcc ggctcgagtt ttactttcgt
    35041 caacagcaac cgggtgataa tggcctctaa tggatgagca tctccgcctt ccagccttgt
    35101 attaagacgg gccagcatct cttgtttcgg aaacgcttca agtactacct gttccgctac
    35161 ccgatacaaa tccagtaacg gtgaatcttc ttctgtctcg ctaccgactc tcgatttcca
    35221 ccacgcccat gctttgcgtt tgaagaataa cgactcaata aacgattgac ctaacggcga
    35281 taattttccg ctatgtaaaa tttccgccca taacagccgc gtccgggcgc tctcaccagg
    35341 aacatatttc agcggttgac cttgcgcatc taccatgctc cgcgcttcga taaccatcac
    35401 taataacgcg aaataatccg ggcaatgacg cttgctccac tgcaacaaca aattatcagc
    35461 ttgtgtagat gggtaactct cccagtatgc gcagagctca gcaagcaggc tttccccctc
    35521 gccgcagtac aaacgttgtg cacactggta ataccgggca tcttcgcttt ctggttcttc
    35581 cgcttgccac tgtttggcta caggaatgag ttccgccata cctgtttgca ccgagctgta
    35641 ccagcgcaac agtttgcgat gtaactttgc atcatcagga atgaaccacg gaccttccat
    35701 cgccaaaaac gcagtgacat agtcaggatg gtaacggcag caacgttcca gcgcgaagta
    35761 atattcaaca gtctggttct gtaacgcgac gggatattcc agaaggctaa ggaaatcgaa
    35821 acaatcacca gcctgaatgg cctcaagaaa agcctccctt tcttcctcat ccattccttc
    35881 gctgtcatta aacgaatgcc agttcaaacg ctctgccagc aaagaagcac aatttaatga
    35941 aagatatcgc gcttctatag cgattgcaca cagcggccag cgtaattgat cgacatcctc
    36001 catgttccag gtatttaatt gctgaataaa tttctgccag gcggagggct gaaaacgatc
    36061 actttctgaa tccagtaatg tcctgaatgc acgtagtatt tcatgttcag cggcggcatc
    36121 tttatcttca ttatctgctt ccccgacagg atttaccgcc agtcgcagcg cctcttcata
    36181 ggcctggcgt aatgctttaa acccctgcgg atcggtttcg ggatgacata acggtaagcg
    36241 agcaagataa gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt cgaggatttg
    36301 ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta ccgggcattc aggctggaca
    36361 gttaatggct gccagggaag cgtggcatag aaacgatcgc ttcccgctgc cagctcttcg
    36421 aacatccgac tcccggcgct atcgtatttg tacagggtta gcgcctcgcg tagcgaagtg
    36481 ttgtcttcgc actgagaaga ttgccagtag agacgtccta acgaataggc ggaaaaatag
    36541 tgcatccagc tgtgatagta atggtgagct cgtacataga cgcgcgattg cagccataaa
    36601 ctctcttctt ccgtcagcca gttgttgagc acaccgaccc gtgacagaaa ccccattcga
    36661 acataatccc aggctttgat tcccccttct ccacagcaca tagcggtttg ggcaacgaat
    36721 tgggtataaa tcaaccctct ctccgagcca ccagcaatta atgctttcca ctcttcaggg
    36781 gaagagcgat gccatgtcag atataagcca gctaaatcat tggcatgacc atcatcagtc
    36841 atctgataaa tagtctgaat taaatcatca cgattattta ttccccagga tgatgagaga
    36901 tcgaattctt ttatcccggt atacattttt ggcgagtcat gcgcgggttt gcaaccctga
    36961 ttaagtattt ccatgggcgc agaaagcgca aaaagaagat attgttc
//
LOCUS       DAPHDX010000036        37005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPHDX010000036 DAPHDX010000000
VERSION     DAPHDX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.036"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..185)
                     /gene="yohP"
                     /locus_tag="RH058_001730"
     CDS             complement(102..185)
                     /gene="yohP"
                     /locus_tag="RH058_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDV3137322.1"
                     /translation="MKIILWAVLFIFLIGLLVVTGVFKMIF"
     gene            409..1845
                     /gene="mdtQ"
                     /locus_tag="RH058_001731"
     CDS             409..1845
                     /gene="mdtQ"
                     /locus_tag="RH058_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV3137323.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1898..2659
                     /locus_tag="RH058_001732"
     CDS             1898..2659
                     /locus_tag="RH058_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3137324.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2789..3367)
                     /locus_tag="RH058_001733"
     CDS             complement(2789..3367)
                     /locus_tag="RH058_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3137325.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3537..4124
                     /locus_tag="RH058_001734"
     CDS             3537..4124
                     /locus_tag="RH058_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV3137326.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4298..5230
                     /gene="pbpG"
                     /locus_tag="RH058_001735"
     CDS             4298..5230
                     /gene="pbpG"
                     /locus_tag="RH058_001735"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV3137327.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5268..6983)
                     /gene="dld"
                     /locus_tag="RH058_001736"
     CDS             complement(5268..6983)
                     /gene="dld"
                     /locus_tag="RH058_001736"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV3137328.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7179..9476
                     /gene="bglX"
                     /locus_tag="RH058_001737"
     CDS             7179..9476
                     /gene="bglX"
                     /locus_tag="RH058_001737"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV3137329.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9687..10604
                     /gene="osmF"
                     /locus_tag="RH058_001738"
     CDS             9687..10604
                     /gene="osmF"
                     /locus_tag="RH058_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV3137330.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10611..11768
                     /gene="yehY"
                     /locus_tag="RH058_001739"
     CDS             10611..11768
                     /gene="yehY"
                     /locus_tag="RH058_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV3137331.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11761..12687
                     /gene="yehX"
                     /locus_tag="RH058_001740"
     CDS             11761..12687
                     /gene="yehX"
                     /locus_tag="RH058_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV3137332.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            12692..13423
                     /gene="yehW"
                     /locus_tag="RH058_001741"
     CDS             12692..13423
                     /gene="yehW"
                     /locus_tag="RH058_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV3137333.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13404..13511)
                     /locus_tag="RH058_001742"
     CDS             complement(13404..13511)
                     /locus_tag="RH058_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV3137334.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(13571..14302)
                     /gene="mlrA"
                     /locus_tag="RH058_001743"
     CDS             complement(13571..14302)
                     /gene="mlrA"
                     /locus_tag="RH058_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV3137335.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14524..16209
                     /gene="btsS"
                     /locus_tag="RH058_001744"
     CDS             14524..16209
                     /gene="btsS"
                     /locus_tag="RH058_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV3137336.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16206..16925
                     /gene="yehT"
                     /locus_tag="RH058_001745"
     CDS             16206..16925
                     /gene="yehT"
                     /locus_tag="RH058_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV3137337.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16972..17442
                     /locus_tag="RH058_001746"
     CDS             16972..17442
                     /locus_tag="RH058_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV3137338.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17483..17944)
                     /locus_tag="RH058_001747"
     CDS             complement(17483..17944)
                     /locus_tag="RH058_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV3137339.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18069..20069)
                     /locus_tag="RH058_001748"
     CDS             complement(18069..20069)
                     /locus_tag="RH058_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDV3137340.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20066..21202)
                     /locus_tag="RH058_001749"
     CDS             complement(20066..21202)
                     /locus_tag="RH058_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV3137341.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21195..23474)
                     /locus_tag="RH058_001750"
     CDS             complement(21195..23474)
                     /locus_tag="RH058_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137342.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(23485..24573)
                     /locus_tag="RH058_001751"
     CDS             complement(23485..24573)
                     /locus_tag="RH058_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV3137343.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(25148..25468)
                     /locus_tag="RH058_001752"
     CDS             complement(25148..25468)
                     /locus_tag="RH058_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137344.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            complement(25529..29161)
                     /locus_tag="RH058_001753"
     CDS             complement(25529..29161)
                     /locus_tag="RH058_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV3137345.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRFIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            complement(29171..32965)
                     /locus_tag="RH058_001754"
     CDS             complement(29171..32965)
                     /locus_tag="RH058_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV3137346.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEEYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            complement(33106..35139)
                     /gene="metG"
                     /locus_tag="RH058_001755"
     CDS             complement(33106..35139)
                     /gene="metG"
                     /locus_tag="RH058_001755"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV3137347.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            35271..36380
                     /gene="apbC"
                     /locus_tag="RH058_001756"
     CDS             35271..36380
                     /gene="apbC"
                     /locus_tag="RH058_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV3137348.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAIVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            36643..36924
                     /locus_tag="RH058_001757"
     CDS             36643..36924
                     /locus_tag="RH058_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV3137349.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         8710 a         9333 c         9866 g         9096 t
ORIGIN      
        1 tgcgccagtg attatatgaa gcaatgcaca tgacaggagc gcaacaggat gagagactga
       61 aatattctcg ttattttcgg acctgatgac attaattaat tttaaaatat catcttaaat
      121 acgccagtca ccaccagtag cccaatcagg aaaataaaca atacagccca gagtataatt
      181 ttcattggcg ttcctttatt tgtgagtgct gaataagtat aggaaaaaac gaagcccttt
      241 gccgttatcc ataaacgatt aagataaatc ggaagatatc attcacttag tgtatatttt
      301 tctggatatg tcgttttatt gctactgatg tattgccacg cgaaactaaa aaatatatcg
      361 caacacacgg taatctccgc gcaccttttc ttacctgatt attcccccat gaatcgtgat
      421 tccttttatc ccgccatcgc ctgttttccg ctgttactga tgctggccgg gtgtgcacct
      481 atgcatgaaa cccgccaggc gttaagccag caaacgcctg ctgcacaagt tgacaccgca
      541 ttacccacgg cgctgaaaaa tggttggcca gacagccaat ggtggctgga gtatcacgat
      601 aatcaactca cttccttaat taacaatgca ctacagaacg caccggatat gcaggtggct
      661 gaacagcgca ttcagctagc tgaagcccag gcgaaggcaa ttgctactca ggatggtccg
      721 cagatcgact tttctgcgga tatggagcgg caaaaaatgt cggcagaagg cttaatgggg
      781 ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc cgtggtacac caacggaact
      841 tttggcttaa cggcgggctg gcatctcgat atctggggaa agaatcgggc ggaggttact
      901 gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg agcaaacccg ccaattgctg
      961 gctggcagcg tagcccgcct gtactgggag tggcaaaccc aggcggcgtt aaacacggtc
     1021 ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga ccgatcgcca gctatatcag
     1081 aacgggatta cttcttcagt tgaaggtgtg gaaaccgata ttaatgccag caaaacccgg
     1141 cagcagctca acgatgtcgc ggggaaaatg aaaattattg aggcacggtt aagcgcactt
     1201 acaaataacc agacaaagtc attgaagctt aaaccggtcg cgttgccgaa agtggcaagc
     1261 cagcttcctg atgaactggg gtactcctta ctggcccggc gggcagattt gcaggcggcg
     1321 cactggtacg ttgagtcatc gctaagcacc attgatgcgg caaaagcggc attttatcct
     1381 gacatcaacc tgatggcctt cctgcaacag gatgcgttgc acttaagcga tctgttccgt
     1441 cattccgcgc agcaaatggg cgttacggca ggcctgacgc tacccatttt cgatagtggt
     1501 cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca acttgtctat cgccagctac
     1561 aacaaagcgg tggttgaagc ggtgaatgac gtggcgcggg cagccagtca ggttcagaca
     1621 cttgcggaga aaaaccagca tcaggcgcaa attgagcgcg atgccttgcg tgtggtaggt
     1681 cttgcgcagg cgcgctttaa cgcgggcatc attgctggtt cccgcgtcag cgaagccaga
     1741 atccccgcgc tgcgtgagcg ggccaatggc ctgttattgc aagggcagtg gctggatgct
     1801 tccattcaac tcactggtgc gttgggcggg ggttacaaac gctgatgata tgcgccttct
     1861 atacttaacg tttattcagc gttaagtgga gaactcgatg gcacaggttg cgattattac
     1921 cgcctccgat tcggggatcg gcaaagagtg cgcgttatta ctggcgcagc aggggtttga
     1981 tattggcatt acctggcact cagatgaaga aggggcaaaa gataccgcgc gtgaggtagt
     2041 tagccacggc gtacgtgcgg agatcgtgca gctggatctc ggtaaactgc cagaaggtgc
     2101 gcaggcgctg gagaaactca ttcaacggct ggggcgtatt gatgtgctgg tgaataatgc
     2161 gggcgcaatg accaaagcgc cgtttcttga tatggctttc gatgagtggc gcaagatttt
     2221 taccgttgat gtcgatggtg catttttatg ctcacaaatt gcggcccgcc agatggtgaa
     2281 acaagggcag ggcggtcgca tcatcaacat tacgtcggta catgaacata cgccgctgcc
     2341 ggatgccagc gcttacacag ccgctaaaca tgcgctcggc gggttaacca aaacgatggc
     2401 gctggagctg gtcaggcata agattttggt gaacgcagtc gcgcctgggg cgatcgccac
     2461 gccaatgaat ggcatggatg acagcgacgt gaagcccgac gcggagcctt cgattcctct
     2521 gcggcgtttt ggcgcaacgc atgagattgc cagcctggta gcgtggctgt gctcagaggg
     2581 cgcaaattac accaccgggc agtcgttgat agtggatggc ggctttatgt tggcgaatcc
     2641 gcagttcaac ccagaatagc gcagtgaggt gcgtggttat ttcaatgggt aaaattttgg
     2701 taggcatgat aagacgcaca agcgtcgcat caggcatggt gcaccggatg tcggatgcgg
     2761 cgcgaacgcc ttacccaggg caatggtttt acgcctgatg atccggcttt ttcttcccgc
     2821 gccgtttcag ccaccagcgc acgcccacca ccagaaccac gaccagaatc aaccagaccc
     2881 agtgcttcaa atgctggtcg agattgtgca accacggcgc aatcacctga ccaccagcgt
     2941 aaccaatagt ggtaaaaatc aacgcccagg caaatgcgcc gagaatattc agcggcagaa
     3001 agattttcgg cggcagctgg ctggcaccaa tcagcgtcgg gccaatcacc cgaaagccat
     3061 acataaagcg cgtaccaatg acaaacagat acggatggcg ttggataagt ttctgcgccc
     3121 gctcaatttt atcctgatgt ttcgagaaac ggcgtaacag cttgccgcca aaccgccgcc
     3181 cgcacagata gagcacctgg tcgccaatca tgccgccaag cgccacagaa agtaccacca
     3241 gcgggaactt taatagcccc tgatgcgccg caacgcctcc cagcaaagtc acggtttcac
     3301 cttccgccag gctaccgatc accagcgcgg cataaccata ttgtgagata agtgtattga
     3361 gatccatagg ccagtctgtc tccctttggt tgccagtaag catacaccct gtgaatcatt
     3421 cacgctgaaa aaggacgttc atcagacagt gaaaattaca tgggcgaaca aaaaataatc
     3481 acaagctgac ttatacttga actgtcaggt tcatgaactc acaacaaggg ggcggtatga
     3541 gccatgtctg gggactgttt tcccatcccg atcgtgaaat gcaggtgatt aatcgcgaaa
     3601 acgaaacgat ttctcaccat tacacccacc acgttttgct gatggcggcg attccggtga
     3661 tttgcgcctt cattggcact acacagattg gctggaattt tggcgatggc actatcctga
     3721 agttatcctg gtttaccgga ctggcgctgg ctgtcttgtt ttacggcgtt atgctggctg
     3781 gggtcgcggt gatggggcgg gtcatctggt ggatggcgcg taattatccg cagcgtccgt
     3841 cacttgcgca ctgcatggtc ttcgcgggct acgttgcaac tccgctgttt ttaagcggtc
     3901 tggtggcgct ttatccactg gtctggttgt gtgcgctggt cggcacggtg gcgctgtttt
     3961 acaccggcta cctgctttac cttggtattc cgagcttcct gaatatcaac aaagaggaag
     4021 gccttagctt ctctagttcg acgctggcga ttggcgtgct ggtactggaa gtgctgctcg
     4081 ccctaaccgt tatactatgg ggctacggtt atcgtttgtt ctagaaagtt ctttgacgtt
     4141 gcattgctgg cgtaaatgtc agcaatgcag catttcctca cgattctcct ttgacgatcg
     4201 cactttttgc tcgttatgat ggcgtcgcta gcctcagtaa atccttactc cggcggtgcg
     4261 caacccgtgc gcgtgaacca ctatctgaat gctcatcatg ccgaaatttc gagtttcttt
     4321 atttagcctg gccctgatgc tggctgtgcc ttttgcaccg caggccgttg cgaaaacggc
     4381 agccgctacc accgcttcac aaccggaaat tgcctccggt agcgcgatga ttgttgatct
     4441 gaataccaac aaagtgatct attcgaacca cccggatctg gtgcgtccga ttgcgtctat
     4501 cagcaaatta atgaccgcga tggttgtgct ggatgcacga ctgccgctgg atgaaaaact
     4561 aaaagtggat atcagccaga cgccggagat gaaaggggtc tattcgcgcg tacgactgaa
     4621 tagcgaaatc agccgtaaag atatgctgtt gctggcgctg atgtcttcag aaaaccgcgc
     4681 ggcggcaagc cttgcacacc attatcccgg tggttacaaa gcctttatta aggcaatgaa
     4741 tgcgaaagcg aaatcgctcg gaatgaacaa cacgcgcttt gttgaaccta ccggattgtc
     4801 ggtgcataac gtttcaactg cccgtgactt aaccaaactg ctcattgcca gcaaacaata
     4861 tccgttgatc gggcagttaa gtactacccg ggaagatatg gcaaccttct ctaacccgac
     4921 gtatacgctg ccgttccgca atactaatca tctggtgtat cgcgataact ggaatattca
     4981 gttaaccaaa accggcttta ccaatgcggc gggccattgt ctggtgatgc gtacggttat
     5041 caataataaa ccggtggcgc tggtagtgat ggacgcgttt ggcaaatata cccattttgc
     5101 cgatgccagc cgcctgcgta cctggattga aaccggtaaa gtgatgcctg tgccggcagc
     5161 agcgttgagc tataaaaaac aaaaagccgc ccaaatggcg gcggcggggc agacggcaca
     5221 gaacgattaa gtgaattcgg atggcgatac tctgccatcc gtaattttta ctccacttcc
     5281 tgccagtttt tccgtttact ggttttaccg atccccggat tcatgctgtt ggtcggatcg
     5341 ttctcgcgat agaacttctg caacgtctcc ggtgctttat acaaatgacc gacgttatgc
     5401 tcggcagggt attgcgcgcc gcgctgctgt agcagttcca gcatctgttc ttttaacgca
     5461 tgcacatcca cgcctttttt cactatgtaa tcctgatgga agacatagca cataaaatgg
     5521 ccgtaataga gcttgtgcac cagctggctg tcgatctccg gcggtaaatg ctcataccac
     5581 tcggtgtcgt tacgccgcag agcgatatcc aacgccagaa tgtcttcgac ttcatcggaa
     5641 tgcaccgcct gataacgaat tgctgcgccc gcagcggcga aacggtgtaa aaacgctttg
     5701 ctgccttcct ccggcgtaca gacaaagaaa tcgccttcgg cctgtttgaa ataatccacc
     5761 aaccacgatt tggcttcgcc cacgccatcg cccgccattt ttaacagcag atgatgctcg
     5821 tatttatcgc gccagttttt catgcgcggc ggtaaatggc tggggaacag gtgaccgaat
     5881 ttttgcatcg cacggtcagt aaaatgcgga cggaagaatt tcactttctc cagcatcgca
     5941 tcggtgcgtc ccttgagatt aaagaagaac ggcatcttgt cggtgcctaa cttatcaatc
     6001 atcaggaagg tgtctttgcc gtatttttcc gcaatatcgt agatatcccg gtgcatatat
     6061 tccccggcaa ccggcagatt ttcgaagtta gccagaatat gacggcggat ttcggtcagc
     6121 acttccggct ggttggtgcc gatataaaac acctgctgat ttttttccgc ttcgaaggta
     6181 tctagacgta ccgcaaagac ggccagcttc ccggcgcaac cagaagattc aaataaccga
     6241 tccggatcgg cgttataacg tgcgggcgtg tcggcttcaa tatcacgaac gcggtggaca
     6301 tagtcataat cgtgggcgtg acgaccatcg tgacgcacat cgtcatcttt gatgcgatca
     6361 tcatccagct tgctaaggat ctgctccggc gtttcgccca gatcaatccc cagatggttc
     6421 accagcgtca gtttgccgtc ttcatttata cgcgcgaata acgacatttc ggtatacgcc
     6481 gggccacgtt gcaccagcga gccgccggag ttgttacaaa taccgccgat aaccgatgcg
     6541 cctatacacg acgatccaat caccgagtgc ggttcgcgtc ccagcggttt gagggctttt
     6601 tccagcgaat agagcgtggt gcccggatag gccagcacct gttcgccctt gccaagaacg
     6661 tgcagcttgt cgagacgcag ggtgctgatg atcacgatat cgcgatcata gtcgttaccg
     6721 tttggcgtcg atccttcggt caggcctgta ttggcagcct gcatcagaat aattttgtcg
     6781 gcggtgacac aggctttcag cacccgccac aattccagta gtgagccagg gaaaacgaca
     6841 gccagcgcgt cgccctgacc agaacggaag cccttgcgat agcgggccgt ttttgcggga
     6901 tcggtgagca ggtgtgaatg accgaccaga cgggcaagtt cattcaaaaa ggctttatta
     6961 tcagttgttg tcatggaaga cattttccac tccttgtggt ggcgaaaaaa tatcacaaac
     7021 tagaatagcg ccttgtatga caattcagcg agtgaatatt ttgaaaattc agacaataaa
     7081 ctgagcattc tttttctctc tccatcatgc ttatggcaaa ctacggtttt tgcaccattt
     7141 tcgctgcctc gtcgggcaac agaggaagaa aaatccatat gaaatggcta tgttcagtag
     7201 gaatcgcggt gagtctggcc ctgcagccag cactggcgga tgatttattc ggcaaccatc
     7261 cattaacgcc cgaagcgcgg gatgcgttcg tcaccgaact gcttaagaaa atgacagttg
     7321 atgagaaaat tggtcagctg cgcttaatca gcgtcggccc ggataacccg aaagaggcga
     7381 tccgcgagat gatcaaagac ggtcaggttg gggcgatttt caacaccgta acccgtcagg
     7441 atatccgcgc catgcaggat caggtgatgg aattaagccg cctgaaaatt cctcttttct
     7501 ttgcttacga cgtgctgcac ggtcagcgca cggtgttccc gattagcctc ggtctggcct
     7561 cgtcttttaa cctcgatgca gtgaaaacgg tcggacgtgt ctctgcttat gaagcggcag
     7621 atgatggcct gaatatgacc tgggcaccga tggtcgatgt ctcgcgcgat ccgcgctggg
     7681 gacgtgcttc cgaaggtttt ggcgaagata cgtatctcac ctcaacaatg ggtaaaacca
     7741 tggtggaagc gatgcagggt aaaagcccgg cagatcgcta ctcggtgatg accagcgtca
     7801 aacactttgc cgcatacggc gcggtagaag gcggtaaaga gtacaacacc gtcgatatga
     7861 gtccgcagcg cctgtttaat gattatatgc cgccgtacaa agcggggctg gacgcaggca
     7921 gcggcgcggt gatggtggcg ctgaactcgc tgaacggcac gccagccacc tccgattcct
     7981 ggctgctgaa agatgttctg cgcgaccagt ggggctttaa aggcatcacc gtttccgatc
     8041 acggtgcaat caaagagctg attaaacatg gcacggcggc agacccggaa gatgcggtgc
     8101 gcgtggcgct gaaatccgga atcaacatga gtatgagcga cgagtattac tcgaagtatc
     8161 tgcctgggtt gatcaaatcc ggcaaagtga cgatggaaga gctggacgat gctgcccgcc
     8221 atgtactgaa cgttaaatat gatatgggat tgtttaacga cccatacagc catttggggc
     8281 cgaaagagtc tgacccggtg gataccaatg ccgaaagccg cctgcaccgt aaagaagcgc
     8341 gtgaagtggc gcgcgaaagc ctggtgttgc tgaaaaaccg tctcgaaacg ttaccgctga
     8401 aaaaatcggc caccattgcg gtggttgggc cactggcgga cagtaaacgt gacgtgatgg
     8461 gcagctggtc cgcagccggt gttgccgatc aatccgtgac cgtactgacc gggatcaaaa
     8521 atgccgtcgg tgaaaacggt aaagtgctgt atgccaaagg ggcgaacgtt accagtgaca
     8581 aaggcattat cgatttcctg aatcagtatg aagaagcggt caaagtcgat ccgcgctcgc
     8641 cgcaagagat gattgatgaa gcggtgcaga cggcgaaaca atctgatgtg gtggtggctg
     8701 tagtcggtga agcacagggg atggcgcacg aagcctccag ccggaccgat atcactattc
     8761 cgcaaagcca acgtgacttg attgcggcgc tgaaagccac cggtaaaccg ctggtgctgg
     8821 tgctgatgaa cgggcgtccg ctggcgctgg tgaaagaaga tcagcaggct gatgcgattc
     8881 tggaaacctg gtttgcgggg actgaaggcg gtaatgcaat tgccgatgta ttgtttggcg
     8941 attacaaccc gtccggcaag ctgccaatgt ccttcccgcg ttctgtcggg cagatcccgg
     9001 tgtactacag ccatctgaat accggtcgcc cgtataatgc cgacaagccg aacaaataca
     9061 cttcgcgtta ttttgatgaa gctaacgggg cgttgtatcc gttcggctat gggctgagct
     9121 acaccacttt caccgtctct gatgtgaaac tttctgcgcc gaccatgaag cgtgacggca
     9181 aagtgactgc cagcgtgcag gtgacgaaca ccggtaagcg cgagggggcc acggtagtgc
     9241 agatgtactt gcaggatgtg acggcttcca tgagtcgccc tgtgaaacag ctgaaaggct
     9301 ttgagaaaat caccctgaaa ccgggcgaaa ctcagactgt cagcttcccg atcgatattg
     9361 aggcgctgaa gttctggaat caacagatga aatatgacgc cgagcctggc aagttcaatg
     9421 tatttatcgg cactgattcc gcacgcgtta agaaaggcga gtttgagttg ctgtaatgct
     9481 ttagtaaggg cgcgacgttt gcgccctttg taggccggat aaggcgctcg cgccgcatcc
     9541 ggcaggaaac gccggaccga atatgcctct taagtattta tctgattctg caactgaatc
     9601 tttccgctca agctaacccc gccattatca actatgcttt tctcttaatt cgctgaaaac
     9661 aacagtaaaa gaatgaggaa agcatcatgc cactcttaaa gctctgggca ggttcactgg
     9721 ttatgttggc agccgtgagc ctgccgctac aggcggcttc ccccgttaaa gtcggttcaa
     9781 aaatcgatac cgaaggtgcg ctactcggca atatcatttt gcaggtgctg gaaagccacg
     9841 gcgtaccgac ggtgaataaa gtgcaactcg gaacgactcc tgtggtgcgc ggggcgatta
     9901 cttccggtga actggatatc tatccggaat ataccggcaa tggcgctttc ttctttaaag
     9961 atgaaaacga tgcagcgtgg aaaaacgcgc agcaaggtta cgagaaagtc aaaaagctcg
    10021 attcggagca caacaagtta atctggctga cgcccgcgcc tgcaaataac acctggacta
    10081 tcgccgtgcg tcaggatgtg gcagagaaaa ataaactcac ttcgcttgcc gacctgagtc
    10141 gttatctgca agagggcggc accttcaaac tggcagcctc ggcagagttt atcgaacgcg
    10201 ccgatgcgtt acccgcgttt gaaaaagcct atggctttaa gctcggtcag gatcagttgc
    10261 tgtcactggc tggtggcgac acggcggtga cgatcaaagc cgctgcccag caaacctctg
    10321 gcgttaatgc tgcaatggct tacggcactg acggcccggt agcggcgctg gggctgcaaa
    10381 ccttaagcga tccgcaaggc gtgcaaccta tctatgcgcc tgcaccagtg gtgcgtgaat
    10441 cggtgttgaa ggagtatccg caaatggcac agtggctaca gccagtcttc gccagcctcg
    10501 atgcaaaaac attgcagcaa ctgaatgcca gcattgcagt tgaaggactg gatgccaaaa
    10561 aagtggctgc cgactacctg aaacaaaaag ggtggacgaa gtaatttccc gtgacttatc
    10621 tacgtattaa tcctgttctg gcgctgctgc tgttgctgac ggtaatcgca gcggcgctgc
    10681 cgtttatcag ttacgcgcct aatcgtttag tttcgggtga ggggcgtcat ctctggcagc
    10741 tgtggccgca aacgatctgg atgctggtgg gcgttggttg cgcctggctg acggcctgtt
    10801 ttattcccgg taaaaaaggc agcatttgtg cactcattct ggcgcaattc gtcttcgtat
    10861 tgctggtgtg gggcgcggga aaggcggcga cacaactggc gcaaaatggc agtgcgctgg
    10921 cgcgtaccag cctcggcagt ggtttctggc tggctgcggc gctggcattg ctggcctgta
    10981 gcgatgccat ccgccgaatc tccacgcatc cgctgtggcg ctggttgttg catatgcaga
    11041 ttgccattat tccgctgtgg ttgctgtact ccggcacgct taacgatctc tcactaatga
    11101 aagaatacgc caaccgtcag gatgtgtttg acgacgcgct ggcacaacat ctgacgttgc
    11161 tgtttggtgc ggtgctgcct gcgttagtga ttggtgtgcc gttgggcatc tggtgctact
    11221 tttccactgc tcggcagggg gcaatttttt ctctgctcaa tgtcattcag accgtgcctt
    11281 cggtggcgct ctttggcctg ttgattgcgc cgcttgccgc gctggtgacg gcctttccgt
    11341 ggctggggaa gctcggcata gcaggaaccg gaatgacacc cgcactgatt gcgctggtgc
    11401 tctatgcctt gctgccgctg gtgcgcggcg tggtagtcgg tttgaaccag atcccgcgcg
    11461 atgtgctgga gagcgccaga gcgatgggca tgagcggggc gcagcgattc ctgcatgttc
    11521 agttaccact ggcgttaccg gtatttctgc gcagcctgcg ggtggtgatg gtgcaaactg
    11581 taggtatggc ggtgattgcg gcgttaatcg gcgcaggcgg ttttggtgcg ctggttttcc
    11641 aggggctgct aagcagcgcc attgatttag tgttgctggg ggtgatcccg gtaattgttc
    11701 tggcggtgct gatcgatgcc ctgttcgatt tgcttatcgc actgctgaag gtgaaacgta
    11761 atgattgaat ttagccatgt cagcaaactg tttggcgcac aaaaagccgt taacgatctt
    11821 aatctcaatt ttcaggaagg gagtttttcg gtgctgattg gcacatctgg ctccggcaaa
    11881 tccaccacct tgaaaatgat taaccgcctg gtggaacatg acagcggaga gatccgcttt
    11941 gccggagaag aaattcgctc gctgccagta ctggagttgc gccgccggat gggctatgcc
    12001 attcaatcta ttggcctatt cccccactgg agcgtggcgc aaaacatcgc taccgtgccg
    12061 caattacaaa aatggtcgcg ggcacggatt gacgatcgta tcgacgaatt aatgacgcta
    12121 ctggggctgg agtcaaattt gcgtgagcgt tatccgcatc agctttccgg tggtcagcag
    12181 caacgtgtgg gagtggcgcg cgcactggct gccgatccgc aagtcttact gatggatgaa
    12241 ccttttggcg cactggaccc ggtaacgcgc ggcgcgttgc aacaagagat gacgcgcatt
    12301 caccgtttgc tggggcgcac tattgtgctg gtcactcatg atattgatga ggcgctaagg
    12361 ctggcagaac atctggtatt gatggatcac ggtgaagtgg tgcagcaggg gaatccgctg
    12421 acgatgctga ctcgtccggc gaatgatttt gtccgccagt tttttggacg tagtgaactg
    12481 ggggtgcgcc tgctttcgtt acgtagtgtg gcggattacg tgcgtcgcga agaacgggca
    12541 gaaggtgagg cactggcaga agagatgacg ctacgcgatg cgctctccct gtttgtcgcg
    12601 cggggatgcg aggtgctgcc ggtgatgaac acgcagggcc agccttgcgg cacgctgcat
    12661 tttcaggatc tgctggagga ggcgtaagcg tatgaaaatg ttgcgcgatc cgctgttctg
    12721 gctcattgct ttgtttgtgg cactgatttt ctggctgcct tacagccagc cgctgtttgc
    12781 tgccttgttc ccacaactgc cacgacccgt ttatcagcaa gaaagttttg cagctctggc
    12841 actggctcat ttctggctgg tgggaatttc gagtttgttt gcggtgatca ttggcactgg
    12901 tgccggaatt gctgtcactc gcccgtgggg cgcggaattt cgcccactgg tggaaactat
    12961 tgccgccgtt ggacagactt ttccgcctgt cgcagtgctg gcgattgccg ttccggtgat
    13021 cggctttggt ctgcaaccag cgattatcgc cttgatcctt tacggtgtgc tgcccgtcct
    13081 gcaggcgaca cttgccgggc tgggagcgat cgatgccagc gtgacagaag ttgcgaaagg
    13141 tatgggaatg agtcgtggtc agcgactgcg taaggtcgag ctaccgctgg cggctccggt
    13201 gattctggcg ggcgtgcgaa cttcggtgat tatcaacatt ggtacggcga cgatcgcctc
    13261 aacggtaggg gccagcacgc tgggtacgcc catcatcatc gggcttagcg gatttaatac
    13321 cgcgtatgtg atccaggggg cgttactggt ggcactggcg gcgatcatcg cagaccgcct
    13381 gtttgaaagg ctggtgcagg cgcttagcca gcacgcaaaa taaaggtata acctgcgagc
    13441 atgacgccac caattccgcc gaacgccata aacaggaaca gggcgatgac cccaatttta
    13501 gctatgcgca taatgcactc cttatgttaa cgaaaggatt gtacagtaaa gcgcatttgt
    13561 taacgaatca ttaaatgccg agtgggaaaa tatcatggcc ttgttcttgc caactggtga
    13621 gttgctgctg ttgggcggag gttcgatttt caccgcacca caccagcaat gtacgacctt
    13681 cgaatagttc agggcgtagt tgattgagcg agtgggcgag gacatcaatg cgccatcctt
    13741 gttgactggc aatccagccc tccagccaca gacgggtggt atcctgaata ttccagccaa
    13801 ccaccagcgc atctttaccc tgttttttac gtgccgaagc cagacaaatg gcgatgtagt
    13861 tgatcagtac gccgtcgagg atcgccagca gcgcctggag agtcggttgt tggcactgaa
    13921 gccgtcggcg cagaggaata aacagatgtg tggtgagtgt ctgggcgggg taatcctgac
    13981 cgcgctcttt gatccacgtt cgcaggctat gcagattgcc gctttgcagg taggtcagta
    14041 atgtttcttg ctgatcgcgc cagccgttct gcacatcaac attttcatta ctgagcagca
    14101 ttttaacttt gctgacctgc acgccgttgt cgatccagcg tttgatctcg cggatacggt
    14161 caatatcggc atcgttgaat agtcgatgac cgccgtctgt ccgttgcggt ttcagcaacc
    14221 cgtaacgcct ctgccacgcg cgtaacgtga cagggttaat atcacaaagc aacgccactt
    14281 caccaattgt gtaaagcgcc atcgtctcac ccttgctcgc gaggtcccgg tttaacttta
    14341 gacgcagttt tgcgaaccag gtagttttgc ccgttttttg tgcatctata gggtgatttt
    14401 atttttgcca ggcgattttg agtgatcgta ctcacgaatt ctcatttttc tgcaagagtt
    14461 caaagaaagt taaacgcagg caatgtatgt tacgcgtttt aaagggaagt gtggtttgcg
    14521 ggtatgtacg attttaatct ggtgttgctg ctgcttcagc agatgtgcgt ttttttagtc
    14581 attgcgtggt taatgagtaa aacgccatta ttcataccgt taatgcaggt cacggttcgt
    14641 ctgccgcata aatttctctg ctacatcgtc ttttccatct tctgcatcat gggcacctgg
    14701 tttgggttgc acattgacga ttctattgcc aatacccgtg cgataggcgc ggtaatgggc
    14761 ggcttactcg gcggtccggt cgtcggtggg ctggttggtc tgaccggcgg cttacatcga
    14821 tattcgatgg ggggcatgac cgcgctaagt tgcatgatct caaccatcgt tgaaggattg
    14881 ctcggcggcc tggtacacag catcctgatc cgtcgcgggc gcactgataa agtctttaac
    14941 cccattaccg ccggtgccgt cacgttcgtc gctgaaatgg tgcaaatgct gatcatcctt
    15001 gcgatcgccc gaccttatga agatgcggtg cgtctggtga gtaatattgc tgcaccaatg
    15061 atggtcacca ataccgtcgg cgcggcgctg tttatgcgta tattgctcga taaacgcgcg
    15121 atgtttgaaa aatacacttc tgctttttct gccactgcgc tgaaagtggc agcctcgacg
    15181 gaaggcattt tgcgacaggg gtttaacgaa gtgaacagca tgaaagtggc tcaggtgctg
    15241 tatcaggagc tggatattgg tgcagtcgcg attaccgatc gagagaaatt gctggccttt
    15301 accggaattg gtgacgacca ccatttaccc ggcaaaccga tttcttcgac ttacacctta
    15361 aaagcgattg aaaccggtga agtggtctac gctgatggca acgaagtacc ttatcgttgc
    15421 tctttgcatc cgcaatgcaa actggggtcg acgctggtaa ttccgttgcg tggcgaaaat
    15481 cagcgcgtga tgggcaccat caaattgtat gaagccaaaa accgtttatt cagttcaata
    15541 aaccgcacgc tgggcgaggg gattgcgcaa ctgctttcgg cgcagattct tgccgggcaa
    15601 tatgagcggc aaaaagcgat gctcacccag tcagaaatca aactgcttca cgcccaggtg
    15661 aatcctcatt ttttgtttaa tgcgcttaac accattaaag cggtgatccg ccgcgacagc
    15721 gaacaggcca gccagctggt gcagtatctt tccacttttt tccgcaaaaa cttaaagcgg
    15781 ccttcggagt ttgttactct cgccgacgaa attgaacatg tgaacgctta tctgcaaatt
    15841 gaaaaggcgc gcttccagtc gcggttgcag gtcaacattg ctattccgca agaattatcc
    15901 cagcagcaat tgcccgcgtt taccctgcaa ccgatagtgg aaaacgccat taaacatggg
    15961 acatcacaac tgctggatac agggcgagtg gcaatcagcg cccgacgtga ggggcaacat
    16021 ttgatgctgg agatcgaaga caatgccggt ttgtatcaac cggtaaccaa tgccagtggg
    16081 ctggggatga atctggtgga taagcgttta cgtgaacggt ttggcgatga ctatggaata
    16141 agcgtcgcct gtgagcctga tagttacacc cgaataacgt tacgactacc atggagggac
    16201 gaggcatgat taaagtctta attgtcgatg atgaaccgtt agcacgggag aacctgcgcg
    16261 tatttttgca ggagcagagc gatattgaaa tcgttggaga gtgttcaaac gccgtggaag
    16321 ggatcggcgc ggtgcataaa ctgcgcccgg atgtgctgtt tctcgatatc cagatgccgc
    16381 gcatcagtgg tctggaaatg gtggggatgc tcgatccgga acatcgtccg tatattgttt
    16441 ttctcaccgc gtttgacgaa tacgctatta aagcctttga agaacatgct tttgattatc
    16501 tgctgaagcc aattgatgaa gcgcgactgg agaaaacgct ggcgcgattg cgtcaggagc
    16561 gcagcaagca ggatgtttcg ctgttaccgg aaaatcaaca ggcgctgaaa tttatccctt
    16621 gtacggggca tagtcggatt tatttgctgc aaatgaaaga tgtggcattt gtcagcagtc
    16681 ggatgagcgg tgtctacgtt accagccacg aagggaaaga gggctttacc gaattgacat
    16741 tacgtaccct ggaaagtcgt acaccactac tgcgctgcca tcgtcagtat ctggttaatc
    16801 tcgcgcattt acaggagatt cgtctggaag ataacggcca ggccgagttg attttgcgta
    16861 atggcttaac cgtgccggtc agccgccgtt atctgaaaag cttaaaagag gcgattggcc
    16921 tgtaaaagac tgctaaaatg gctttttgcc tcatcaacac ctgaaggcct catgctaagt
    16981 aacgatattc tgcgcagcgt gcgctacatt ttgaaagcca ataataatga cctggtgcgt
    17041 attctggcgc tgggtaatgt cgaagccacc gcggaacaga tcgccgtctg gctacgtaaa
    17101 gaagacgaag agggttttca gcgttgtccg gacattgttt tgtcgtcatt cctcaacggc
    17161 ctgatttatg aaaaacgcgg caaggatgag tctgctccgg cactggagcc ggaacgtcgc
    17221 attaataaca acatcgtgct gaaaaaatta cgcatcgcgt tttcgctgaa aaccgatgac
    17281 attctggcta tcctcaccga acagcagttc cgcgtttcga tgccggaaat tacagcgatg
    17341 atgcgcgcac cggatcataa aaacttccgc gaatgcggcg atcaattttt acgttatttt
    17401 ctgcgtggac tggcagcgcg ccagcatgtg aagaaaagct aagacgggta tggcggccat
    17461 gcgaaacatg gccgccgaca gattatttca cttctttaaa accagcggct ttcatcacca
    17521 gttccatttg cgccatagtg ataccttttt tggcatcttc agcagaaacg ttgattcctg
    17581 aaataccctg cagggcttta aaatccactt tttccatatc gatagtcacg ttttcctgcg
    17641 cgtaggtatc tgtataggtt aatttttctt caacacccgc gatgtttttg tatttggcgc
    17701 ttaacggctc aagtgtcttg gcagcgtctt ctttggtggt tgcaccaata gaagcaaatt
    17761 gaattttggt ttcagaagat tgcttaagca ccttgtcacc tttgtagaca taggtaatgg
    17821 caatttcagt gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc
    17881 cagcaagaga gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct
    17941 tcatgtaaaa ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata
    18001 gaatatgcct aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa
    18061 gcactatttc atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg
    18121 ttattcattg cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt
    18181 gccagccgat aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata
    18241 gcgctctgtt cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag
    18301 cgcatcagca actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt
    18361 gccacaggcg cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt
    18421 ggttccagtg tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg
    18481 tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat
    18541 gccggaaggg ataacgtcag gagattatct tcaccttcgc cgctgattac ctgcgcatcc
    18601 agcgtctggc ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat
    18661 aagttatcga cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc
    18721 cgcaaagcca caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc
    18781 gacagcggca caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca
    18841 ctgcgttttg ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta
    18901 aacagccgac gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa
    18961 aggtgcaaaa tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca
    19021 atatcctgcc agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct
    19081 ccggcaatgc cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg
    19141 tcgcgccggg ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt
    19201 aagcattctc cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt
    19261 agctgtcgta gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg
    19321 cggttgaatg ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc
    19381 tggcttaatt gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg
    19441 tcagatgatg tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc
    19501 tgcgctttgg cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct
    19561 tcaatacaat cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc
    19621 atcggtaagc gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct
    19681 ttagcgacca gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagttcttcc
    19741 agccagattg ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt
    19801 cgttgataac ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg
    19861 cactgagcct ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag
    19921 gtggcgatta aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc
    19981 ttaagactgc gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt
    20041 tctaataatt ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca
    20101 gctcgcccgg cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat
    20161 cgcggtcata gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc
    20221 cgctctggac acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa
    20281 aatcgctcac gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact
    20341 ccacggcact ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg
    20401 gatcggcaac gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac
    20461 gaatgccggg taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca
    20521 ccatcgatcc gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa
    20581 tgcggctgtt aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt
    20641 gcaggttggc gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc
    20701 ggcgacggcg atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca
    20761 taatttcttc aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca
    20821 tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca
    20881 gcacgtcgag attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca
    20941 gtcgctcaat cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg
    21001 tgagatttga gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc
    21061 gcccatacag ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa
    21121 gcgtcgtttc tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca
    21181 gaagatcgtt cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc
    21241 tgttcgagtt gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc
    21301 gccgaaacgg gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg
    21361 cgttctcgtg gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa
    21421 tcggcatcac ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag
    21481 gccagttgat ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg
    21541 gataactgcg ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc
    21601 atcagtgcgc catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt
    21661 cgctccagta aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt
    21721 agatcgcgca ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt
    21781 tcatctggtc tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac
    21841 gtcgtttgta atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca
    21901 tatagcactt ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat
    21961 gtgagttgct ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta
    22021 agacatgtgg tgatactgcc gattccaccc gcgtccagca tatcggcttc taatttatgg
    22081 cgtgcggctt cctgaagtgt tgcaccaaag catgcggcct caatcaatgc cgcatgctgg
    22141 ctaagcgggc gggttaattt ccagtgttct tcaccgttgc ctgcaagtgt cagtgtactt
    22201 ccctgctggc gtacaatccc agggatctcc agtattgcca gccgatgtaa cacctgactt
    22261 tgcgccagcc catcgggggt aaagcgattt aaggtaagct ccgccggaag agagattgcg
    22321 gtacgttcca gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt ggaaggggca
    22381 agtttaccga atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta
    22441 tcgggatgaa taacgccgcg atagctccac ggcaacggtg cattcagggc ttctttaatc
    22501 aacgagcctg ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac
    22561 tgtgccagtg ccattgcatg cagatgtgcg gcagccatat ccgcggtcga agcaggcaag
    22621 ttgtgctggc gcaaacgggt gagaaccgtt tttagcagtt gttcaccggc ctgctgtaag
    22681 ccccactgcc agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc
    22741 agcacatcaa ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc
    22801 agcgagggca attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt
    22861 gccggagcgt gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc
    22921 catgcaatcc agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa
    22981 tcgccccgca gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt
    23041 tcatcttcga acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc
    23101 agtaaggcct ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc
    23161 cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc
    23221 tgccactccg gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg
    23281 tcctgatact ggcaataact gtaaatagct accggaagct ggtgggaaaa aaaaagttcg
    23341 tctacccggt cattaaaatc agccgggcct tcaatcaaca cgtatcgtgg ccgctggctt
    23401 tcgattaacg atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt
    23461 aacggctcgc tcatgcctct ttcctcacgg caggcggtgg cgggcttgat aataagcctg
    23521 ccagtgagct tctttatgtg tggcgacacg ctgttcgaag taacggcgca gacgagcgcg
    23581 atcttcttcg ttatctttga cgatggttcc ggcaatacag tccaccaggt cggcaggctc
    23641 ccccgcgcga ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc
    23701 ttcagcggtg gacataatcg ctgtcagtgt atccattgac gttttctttt cgccgttggc
    23761 acgcagatcg cggaaggtgc gtaccagtaa ctccagtact gcgtcaggca ctttatgcgg
    23821 aataccgcta tgggccagca aacgcgccga agcactggcg accagttcca gctcctgggc
    23881 gaaatccata atcggaaaga cggtttcaaa atcgaagcga cgctttagcg cggcactcat
    23941 ctcattgacg ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc
    24001 atagagctga ctggcttcac cggtgagttc cggcaccgtc atcaccctgt cagaaagcat
    24061 acctaacaga cagtcttgta cttccagcgg tgtacgcgtt atctcttcaa aacggacgat
    24121 tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc agtgcttccg ttgacggacc
    24181 atggttgata agcagggcgt agttccagcc gtacttgatt tgatcttcgg tagtagatgc
    24241 gccgccctgg atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag
    24301 gagagatttt gcggtgccag gttcccccac caacatcaaa ccgcgcccgg tggcaagagt
    24361 gactaacatg cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc
    24421 gctatcgcca aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca
    24481 aggtgcgtta tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg
    24541 cggacgctgc agatgattat tctgcggtga catatattca tccctgaaat gtattaatga
    24601 aataaacgat attttacgcg caataaatgt ttggcgcatt agttgtttga gcgaaaaata
    24661 tctggaaagc agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta
    24721 ctactttcag gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa
    24781 ccacggctgg accccgttgc aattaagata atgagattta taatccagac cgttgggacg
    24841 ttgcgccgga tcgaatctat ttcaagtcct ggctaccgga cgggtggtgc tgcaggtggt
    24901 gccctgatcc ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt
    24961 gtaagggtaa ggtgctggtg ttgcgctcct taataaggag agcggatgga cacgctgaca
    25021 caaaagttaa ccgtgctcat tgccgtactg gagctattag tgtctctgtt acggttgatt
    25081 gatttgttga agtaatgggc aaattgaaac gcaactaagg gggaaaaccg cttccccctt
    25141 taataaacta atattctatt agttggttga atatattctg tagttcacga ttaaaaattg
    25201 ctattacagt ttcaggtgat tcacctcgta atttccgctc atcaatacac tcatctctta
    25261 tataaatcaa cgcatcattt attagcgggt cagcatcttc tcgatcacgt tgatgaaatg
    25321 ttggacgtag ccggaatcgc tccagtcgat agacggaacc tgtaaataat tcagagcatt
    25381 tatggatctc aagtctgaag gaaaacggat cttcaggtac agggacctca taatcgtaaa
    25441 tagcaaccag ctctttttgc acgatcatcg ctttccccgc tggtaagtcg tgttgataac
    25501 gggcagttgc atcactgccc gctgtgtttt aatggaaaat gacgtcaggt gcgcttaacg
    25561 cctcactcaa atcaaacaca tcaagttcgg caaaagtgac ggatttattg tcactgtaca
    25621 ctcgaagaga acggaagctc attagcgctt tgctatcaac attgtagtgg ttaaaaccgg
    25681 gtgaaaactt aaggaccagc cggtactcac ccaggtcata gacaaatgcg tcctcatagc
    25741 ctgactccca gcctttgtat cgcattccca taagttgccc aacggtagca gatttacctt
    25801 cccagcgatt caaactatta ctggctgact cgtcaggcgt gagcagcacc gtgcgccgtg
    25861 tcaactggcg aagtggcggt ataatttcgt aatcggcaaa aagctgggca aattcactgc
    25921 gcatttctgc tgtcatttct aacgggtggg caatgccaat cagagcatcc gcaggcaggt
    25981 caattggctc atcctgcgca ttgcagaact ccccctctgc ggccacacga aaggcgttga
    26041 gtaaacgacg cggttcattt gccggataca cgccccatat gaatcgctgg gtaaccaggc
    26101 gggtaaaggg atgattaaca aacagagtat gaaaatctgc cagcgaccag cgtcgggtgg
    26161 tacgtagggc agcttcaaga cgcgggagca ggtttttgct cacctgagta gcatcttttt
    26221 ttagcccttt tagtcgggcc agtgcctcgg gcgctttcag ttgatcgtca tcggcgcgca
    26281 accggggaac gcttttctgg cgaacgtttt gctgatcaaa gataaccggg ttgaggtttt
    26341 catcgaagcg aacggtaaac tgccggggac caaaatcaaa actcaacgcc tgcggatcat
    26401 caaggcccag ggttgggact aatctgtccg ctagctcttc ctggcttagc ccgcgatttt
    26461 ctgcgaccac ctgaagaaat tccgccgcgt tatcacgtaa ggggcgggat ttagcccgtt
    26521 gcgatatatg atgcagctgt atcagcgcca tatcattatt catcagggta aggatgttca
    26581 ggccgctgac agcacgggca gatttgcctt cttgtggcca ggcgaggatc tgtgtcgtca
    26641 gatcccgtgc cgtgctttcg tcaccaaaga gacttaacgc cagaaatgcc cagttgtctt
    26701 ttgcgggggc accagcttgt tgccacgcag tgaagagatc ccaggcaaaa gctgccagcg
    26761 tttgtggctg gcagaacgtt ttcagttgtt ccagcccgct ataaaaacgt cccccctggg
    26821 taaagcgcag catttcgccg ataatttcca gagcgtcacc ggtaacaggc tgattattag
    26881 tgataagtcg aggcctggac cacattgccg gatgccagaa atcaggggct tttggaatgc
    26941 gtgccgggta aatgtccatt ggaccctgtt taagcaactg ctccagggca gaccatacat
    27001 ctgtacgctg ccagcggttt gccacggttt gtagcaattc gccatgtccc tgttcgtaca
    27061 gtaaacgcag ggcaagtaat gcggcttcgc tattatcgct gggtttggta aagacaagag
    27121 ggataagtgc actagccgta tgttccggcc attgtaaaat ccactggcga gccagatcac
    27181 gctgcgccgc gaaacgtcgc cagacgcggg caacaggcag cgccagttct gttgcgccaa
    27241 aatttaaaat taacggaaat gtttcttttg gacgatgtga aaaagccaac agcagacctg
    27301 gtagcgcatc caatccgagg atactgagaa aatagtcctc acccgcgaac agatgctttt
    27361 tctcaatgat acgcagccag aaggtcaaag ccatctcacg cggcaactgt gccaataaat
    27421 acaggttcca gacactcttt tctggacgat aacgtgagaa gttgtcattt tcccatatgt
    27481 taattaacgc ttctatgtga ccgctgcgga gcgcctgttc tgctaattgt gcttgcgggg
    27541 agttccagcc attcaggatc tcttcaaccc gcccctggta gtaatgccac tgaacaatga
    27601 gtgaattgcg tgcagcatcc agttttccgg ggagtatata attcagaaac agccatacat
    27661 catccccaaa acccagttcc tgtaatacaa ctcgtccact tttttgttcc atgcgttccg
    27721 caagagattc cgtgctgaaa taacgggcag actcagtggc ggcaagcctc tcttgttcgc
    27781 ctggttgcca gtaaatttgc ggagttaatt cgagcggtgc caaatcaaga cgtggagctg
    27841 tcattttttt cttactacgc cacggtggtg aaacgagcat ttcaggcaga ctgctgttat
    27901 cggtgctatc aataaccggt tttggcggtg ccgataaata actattcagc actgccctgg
    27961 cttgagtact taaccacggc atcacccttt ctgctagttg tggctgcgat gctatcagcg
    28021 tttgtaacag cgtacgccag tattcatcgc gtgctttttg ctgtgcaggc agctttttgt
    28081 cttcgatgat tggataacct gggcgggctg gtggttcttt taacgccagc agttctgcca
    28141 gtgcggcgag tgtcgcatgg gggaagtttt tactgtattt agccacacgt tgcaggctgg
    28201 gtttgttttt atcagcaact aacagcaagg tgcggatgac ttgcggatgg ttaatttgta
    28261 ccagcagact gccgcaatct tctttatggg catatatcgc gaggcgcgga acggctgcca
    28321 gtccttgttc gcgcaaaagg gcggcgcagg ccgcgtagcc gaagtggttt tcatgcgaca
    28381 tgtagctggc ttctcgatcg ctaaatacgt ccaggcccca gcatcgttcg agttttttta
    28441 ccgccgtatt atcagtagca atgaccttta accactcctt tgaatggaga cttgaccggg
    28501 agctttcaag actggcgagt tcattggcta tttcaggttt ttccgggagg agtagtgcaa
    28561 taaaaggacg gcgaattttg ggtattcctg gcagtgcggc aatgagtttg tcggcgcaac
    28621 gctgccagct gctttcctct gccagagaga gatgcttacg cagtcggaga tcgaattcgt
    28681 tataggtttc tgagcgataa ccatagccat aatcctcgtc ctgatactgc aatgtcacta
    28741 gcagaggatc actctcataa catcgtgtaa taaactggcg tgcaatcacc acctcagtcg
    28801 catattccag tccctcttgt tggacgattt cgtcgagcaa ctgttggctg tcattttgcg
    28861 cgatacagac tagcgccgcc attgtccggg ccgggattga gggtttgtgt tcgccgatca
    28921 agttgatggc ctgcgtaatg gcggactgcc actccagtga aaaattctca taacgccaga
    28981 caatgttttt tttcttctca gctttttgta gcaattcatc acccagtgtc tgaatgatgc
    29041 tttcagtgat aacaggcact ggttcgcccg gccaattgcg atggctgagt ggtgttttgc
    29101 cttttttata tttcagttcc agttgggcgt tatccgccag ccacggtaat tccttgtcca
    29161 tgctctttcc ttattcaaac agggcttcaa tatcgttaat cagctcgctg gcggtgatgg
    29221 catcgagtac ggagaactgc gcttcctgtg ttgaattact cccccagcca tagctttctg
    29281 ctttgccttc ccatagccac agcttgctta agagttgctc ggcgctaagt tcggctggcg
    29341 acataccaac tgcaaagcct tcatcgattt ccatgattaa cgaccagcgg cccagaggtt
    29401 tgatcatcca gccgatccag ccaccatcct gcggttcacc acgcacccaa cctttattcg
    29461 ccagtcccat gacccgaccg ctcgggcatt ttctgcctgc ccagcgggtc agttcactgg
    29521 cattgcgctc ggcttctgtc agggcgtagc tgttacggtc gagctggcgg aacggtggta
    29581 acagttcgta gtcggcaaaa agctgaccaa aggcggcagc atccgttggt gatatttcca
    29641 gaacgtgagg agtgccgata gagatatcgc cttccggcag ggtgaaaaga tcatcgtcag
    29701 cggtgctgta gctgttatct tctgctacgc gaaagcaagc cagtagctgg ttttcggcgc
    29761 tataaacgcc ccaaatcaga cggcgggtta agtgacgaac cagcggatgc tcaaccagga
    29821 agagctggaa gttttccagc gaccagcggc ggcgcaggca catggcggat tccagccttg
    29881 ctacctgctg ggcggcgatg gtacgcgcat cttttttcag caatttgtag cggttaaccg
    29941 catcgttcgc ccgcgtttca tcatcgcttt tgttcggttt gggcaggtct ttcaggcggc
    30001 tgccggaaac atcacgcaca aacggtttta aggtttcatc aaagctgacg gtgaactgcc
    30061 gtgggccaaa atccagcagc agcgaaccgt tatcatccag accgagatcc ggtgctaacc
    30121 gatcttcaag ctccgccacc gtgagttcgc ggctctcggc aatgtcggca attttttctt
    30181 ttgcccgctc ctgtaatgct ttgaatttca gtttctgggc gatgccgtta agctgcataa
    30241 gggcgatatc actaccgata gcagcgagaa tatccaaccc aacggtggcg cgtttatgct
    30301 gggattcacc aggccaggcg cgtattaatg gcgtcagttt gcgggcggtg tcatcgttac
    30361 cgagaacgcc taacgcagtg aacgcccaac tctctttcga cggcgcgcca gcggtctgcc
    30421 aggcggtaaa cagatcccag gcaaatcccg ccagtgagtc ggcggaacag acgtctttca
    30481 cctgcaataa tcccggatac agagcctctt cctgagggaa gcggagcatt tcaccgaggt
    30541 ggaggagggc gctatctggc agtgattggg catttgcttt taataccggg cgcgtccaga
    30601 gcgatggctg ataaaaggcc ggaagagtgg ggatttttgt cgggtgatta tctaagggat
    30661 cgagcgcaag cagagcgttc accgcatcgg ttacttccgg ctggttataa cgtcgggcga
    30721 tttcttgcag taatggctga tgaccgtttt cggtaagcat acgcaaggca gcgcgggcgt
    30781 tatcctgtgc ttcaccggct ttgccgagcg ccgcaggcag caggccggta agggcatgtt
    30841 ccgggtattt caacagccag ctacgggcgt tttcgcgtag cgttttcagt ttgttgaagg
    30901 cacgggcgac ggcaggagcc agctcactcg ctgcgaagta attcgtgatg ggcaaggctt
    30961 cttgtggata gcgtgcaagt gagtttacta acccaggtaa ccctttgagt ccaaaatgta
    31021 acatggcgta ttcggtatcg gtatgtggag atagggtaag ggcattccat gcttttactg
    31081 ctttctccag cggcaatgtt gtcaaaatcc ccaactgcca ataatgcccg gtaggtgaat
    31141 gaaacacctt aaattcactt agtaaggttg aataatcttc atttagccat gcctcaacta
    31201 ccgcttcggg agctttaata tatgtaccag cgatccgtcg ttgaaaaccc atccttgcca
    31261 acaggttggt agtactttct tcatggctaa ccgtgatgtg ttttgaatac cagatatatt
    31321 tcgccaaaag ttgattactg atttcttctg tcagataaca gattggctca atgcctaatg
    31381 gcgctaaatc cagcaccgga atcggcgatt ttttcttttt cgaaagccag ggaggggaga
    31441 ctactacggc tggcagtaga tcggcgctgg catggtttga gggctgtgtc agttgttgct
    31501 ggcatgattt cagtacggca actgcgggtg tcgagagcca gggaatgacc tgttctgcca
    31561 gtgctggttg tgagataagc attgtcatta gcataatgcg ccaactgttc tcttcttttt
    31621 gcccaagaag ttccgtcagt gccgccattg ctgcatgtgg gaacgcagcg caggctttcg
    31681 tcatccgatc gtgacagcgt ttagtatgcc cggctacacg tataagcagt gttaatgcga
    31741 acggatgatt gatatgacgc aacacatcgg cgcagtagtc acttgcggca tagggagcaa
    31801 atcggggcag ggctgcaaca ccttgctcct gtaatacggt ggctgaccat atcgcaccac
    31861 aatagtaatc atcaaaaatg gcatatggct cgtgatattc ttctaatgag gcaagaatgt
    31921 gctcatcagt cgccactatt tttaaccact caagcgaggg taattttttt tgccccagta
    31981 gacgctgggc aatttcatgt gcaatttctg gtttttcggg taataacaaa gcaattaatg
    32041 gttgccgcca ttctggaata tgtggaattg cggcaataag tttttgtgca catttttgcc
    32101 agagagactc ttccgccagt gataaatgtt tacgtaggcg taattcaaat ctggaatatt
    32161 gctccaagta gtcaggtgca atgccagacg gcagaataat aatgacatta ttcgcataat
    32221 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagaccgc
    32281 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
    32341 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
    32401 tctgactgat cgcctgggcg acggtgtttt gccattctaa agaacaggcg ctgaagtcaa
    32461 atgtggctgt tttattatcc cgttgcgaca cactacaggc taagcgacgt aattgggtca
    32521 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
    32581 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccacggtaaa acagcatctg
    32641 tatttttgtc aggcgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
    32701 ataagtccgc taccttgaga gagcccgtta ccgtttggga aggttgcaaa gaattatcct
    32761 tcgcttgctc cacatacccc ttcttcacct tctccgcaat cagcttaagc tccgcttttg
    32821 ccgctgccgc agcatctgaa aaacttttta tctggctttg ccctttggtg ccaacttttc
    32881 cccaactgat atgcaactcg tttccctgtt gctcaactgc ccagaattta tgtgattttt
    32941 cgtcctgata gataaagtgt ctcattctgc atccctgtaa ttttatccag ttgaatgcag
    33001 atgctaccag tatttatgcg ggttagagag agacaaaatc gcaagaaaac aactttagga
    33061 atttataaag aaaaggcgct gtcgatgcag cgccttaaag ggggattatt tcacctgatg
    33121 gcctggttta gcaccagcat ccgggcttaa caggaagata tctttcccgc caggaccagc
    33181 agccattacc atgccttcgg agataccaaa gcgcatttta cgcggtgcca ggttggccac
    33241 cataatggtg tgacgcccga tcagtgcctg cggatccggg taggcagaac gaataccgga
    33301 gaagacattg cgtttttcac cgccgagatc cagtgtcagg cgcagcagtt tgtcagaacc
    33361 ttcaacaaac tctgcgtttt cgatcagcgc cacgcgcagg tcaacttttg cgaagtcgtc
    33421 aaaggtgatg gtttcctgaa tcggatcatc tgccagtggg ccagtaactg gcgcggcagc
    33481 tgctttcact tcttctttag acgcttccac cagtgcttca acctgcttca tatcgatgcg
    33541 gttatacagc gctttgaacg gattcacttt gtggcccagc agcggttgct ggataccatc
    33601 ccaggtcagt tctgtattga ggaatgcttc tgcgcgctcg gtcagtttcg gcagtaccgg
    33661 cttcaggtaa gtcatcagca cgcggaacag gtttatgccc attgagcaaa ttgcctgcag
    33721 gtcggcatcg cggccttcct gttttgccac cacccacgga gcctgctcgt cgacatagcg
    33781 gttagccaga tcagccagcg ccatgatttc gcgcactgct ttaccaaatt cgcggctttc
    33841 ccacgcttca ccaatcactt cagcggcatc agtgaaggtt ttgtacaact gcgggtcagc
    33901 caattcgctt gccagcacgc cgtcaaaacg cttgttgata aagcccgcat tacgagaggc
    33961 caggttaacc actttgttca cgatatcggc attcacacgc tgaacgaaat cctccaggtt
    34021 gagatcgata tcatcaatgc gcgaagagag tttcgcagtg tagtagtaac gcaggctgtc
    34081 agcgtcaaaa tgattcagcc aggtgctggc tttaataaag gtgccgcgag acttggacat
    34141 ctttgcgccg ttcaccgtca catagccatg aacaaacagg ttggtcggct tgcggaagtt
    34201 gctgccttcc agcatggcag gccagaacag gctgtggaag taaacaatat ctttaccgat
    34261 gaagtggtac agctcggcgg tggagtcttt cttccagtat tcatcgaagc ttacgctgtc
    34321 gccgcgcttg tcgcacagat tcttgaaaga acccatgtag ccaatcggtg cgtccagcca
    34381 gacgtagaaa tatttgcccg gcgcgttcgg aatttcaaaa ccaaagtatg gcgcgtcgcg
    34441 ggagatatcc cactgttgca ggccggattc aaaccactcc tgcattttat ttgccacctg
    34501 ctcctgcaac gcaccgctgc gggtccatgc ctgcaacatt tcgctgaaag agggcagatc
    34561 aaagaagaag tgttcagaat cacgcattac cggcgtagcg ccagaaacca ccgatttcgg
    34621 ctcgatcaat tcagtcgggc tgtaggtcgc gccgcagact tcgcagttat cgccgtattg
    34681 atccggggat ttacatttcg ggcaggtgcc tttcacaaaa cggtccggca ggaacatgcc
    34741 tttttccgga tcgtacagct gagagatggt gcggttttta ataaaaccgt tttctttcag
    34801 gcgagagtag ataagttctg acaactggcg gttctcttcg ctgtgcgtcg agtgatagtt
    34861 gtcatagctg atgttaaagc ctgcgaaatc agtctgatgc tcctgactca tttcgccaat
    34921 catctgctcc ggggtgatac cgagctgctg agctttcagc atgatcggtg taccgtgggc
    34981 atcgtcggcg cagatgaagt tgacctcgtg gccgcgcatt cgctggtaac ggacccagac
    35041 atcagcctgg atgtgctcca gcatatggcc gaggtggatt gagccgttag cgtacggcag
    35101 tgcgcacgtc accagaattt tcttcgcgac ttgagtcata gtaggcatta cttcttaata
    35161 gtgaaaaggg gttagatagt accaaatggg aaaatgttaa gtaagtactt ctctatattc
    35221 ttcaggtaaa ctatcagctg atggtttatc tatacaaaaa aggagtcggg atgaacgaac
    35281 aatcccaggc caaatcacca gaagccttgc gcgcaatggt cgccgggaca ctggccaatt
    35341 ttcagcaccc aaccctgaag cataacctca ccacgctgaa agcgttgcac catgtcgcct
    35401 ggatggacga cacgttgcat gtggaactgg ttatgccatt cgtctggcat agcgcgtttg
    35461 aagagttaaa agagcaatgt agcgccgaac tgctgcgtat caccggggcg aaagctatcg
    35521 actggaaact gtcgcacaat atcgccacct taaaacgcgt gaagaatcag ccgggtatta
    35581 atggtgtgaa gaacatcatt gcggtcagtt ccggtaaagg cggcgtgggt aaatcctcca
    35641 cggcggtaaa cctggcgctg gcactggcgg cggaaggggc gaaagtcggc attctggacg
    35701 ccgatatcta cggcccatca attcccacca tgctgggcgc agaaaatcag cgtccaactt
    35761 cgccggacgg tacccatatg gcaccgatta tgtctcatgg cctggcaacc aattcaattg
    35821 gttatctggt cactgacgac aatgcgatgg tgtggcgtgg accgatggcg agcaaggcgt
    35881 taatgcagat gttgcaggaa accttgtggc cggatctcga ctatctggtg cttgatatgc
    35941 cgccaggcac cggtgacatt caattgacgc tggcgcaaaa cattccagta actggtgcaa
    36001 tcgtggtcac caccccgcag gacatcgcgt tgatcgatgc gaaaaaaggc attgtgatgt
    36061 tcgagaaagt cgaggttccg gtgcttggta ttgtcgagaa catgagtgtg catatttgca
    36121 gtaactgtgg tcaccatgaa ccgattttcg gtaccggtgg tgcagagaaa ctggctgaaa
    36181 aataccacac acagctgctg gggcagatgc cgttacacat ctccttacgt gaggatctcg
    36241 ataaaggcac gccaacggtt atcagccgtc cggagagcga atttaccgct atctaccgcc
    36301 agctggctga ccgcgttgca gctcagctct actggcaggg tgaagtcatt ccaggcgaga
    36361 tttccttccg cgcggtctaa cgcctattaa ccattctggt tattcttcat acaccgcaga
    36421 gatgttacat tgatgcggtg tattagtttt tgccgcatta atgaacactt atttaatact
    36481 gcgaatattc tgcggacctt cgtccgaaac tctacaaagt cgattgatca tacaggcaat
    36541 gcttcattat catccttcgt ggctgtgtag tggatgtaca gctatagttt gttatgcact
    36601 cattctatcc aggcataacc tttactacag ggataacgtt caatgaataa gtattggctg
    36661 tcaggaatta tttttcttgc ctatggattg gcttctcccg cattctcttc agagactgcc
    36721 acattaacga ttaatggcag gatcagtccc ccaacatgca gtatggctat ggttaatggt
    36781 caacctcaac agcattgtgg tcagttaacg tataatgttg acacccgtca tctgttttct
    36841 tcgcccgtca aaggtgtaac gacagaagtg gttgttgcag gtagcgatag caaacgtcga
    36901 atcgtgttaa atcgctacga ttaatttggc aacctgattt taattgcatt tagaaagccg
    36961 aaatcattta tataaatgat ttcggctttt ttattgatat caaca
//
LOCUS       DAPHDX010000037        36638 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPHDX010000037 DAPHDX010000000
VERSION     DAPHDX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.037"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..674
                     /locus_tag="RH058_001758"
     CDS             <1..674
                     /locus_tag="RH058_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3137350.1"
                     /translation="HEARILNSIWLITALGLVTTAGLAWVAKYLEITATKYDSPQMYV
                     AIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPAIIVVFSTAGAM
                     FLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISCLMIVLWSG
                     IISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIAITLVWQRH
                     TRFFH"
     gene            complement(710..1666)
                     /locus_tag="RH058_001759"
     CDS             complement(710..1666)
                     /locus_tag="RH058_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV3137351.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1666..2907)
                     /gene="clsB"
                     /locus_tag="RH058_001760"
     CDS             complement(1666..2907)
                     /gene="clsB"
                     /locus_tag="RH058_001760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV3137352.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2904..3665)
                     /locus_tag="RH058_001761"
     CDS             complement(2904..3665)
                     /locus_tag="RH058_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV3137353.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3798..4208
                     /locus_tag="RH058_001762"
     CDS             3798..4208
                     /locus_tag="RH058_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV3137354.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4170..5276)
                     /locus_tag="RH058_001763"
     CDS             complement(4170..5276)
                     /locus_tag="RH058_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3137355.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5287..6420)
                     /locus_tag="RH058_001764"
     CDS             complement(5287..6420)
                     /locus_tag="RH058_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3137356.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6413..8149)
                     /locus_tag="RH058_001765"
     CDS             complement(6413..8149)
                     /locus_tag="RH058_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3137357.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8142..9137)
                     /gene="hlyD"
                     /locus_tag="RH058_001766"
     CDS             complement(8142..9137)
                     /gene="hlyD"
                     /locus_tag="RH058_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV3137358.1"
                     /translation="MKKPVVIGLAVVALAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9140..9811)
                     /gene="cecR"
                     /locus_tag="RH058_001767"
     CDS             complement(9140..9811)
                     /gene="cecR"
                     /locus_tag="RH058_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV3137359.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10040..11404
                     /gene="rhlE"
                     /locus_tag="RH058_001768"
     CDS             10040..11404
                     /gene="rhlE"
                     /locus_tag="RH058_001768"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV3137360.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11636..12118)
                     /gene="ybiA"
                     /locus_tag="RH058_001769"
     CDS             complement(11636..12118)
                     /gene="ybiA"
                     /locus_tag="RH058_001769"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDV3137361.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12238..14388
                     /gene="dinG"
                     /locus_tag="RH058_001770"
     CDS             12238..14388
                     /gene="dinG"
                     /locus_tag="RH058_001770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV3137362.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14416..15378
                     /gene="ybiB"
                     /locus_tag="RH058_001771"
     CDS             14416..15378
                     /gene="ybiB"
                     /locus_tag="RH058_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV3137363.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15519..16604
                     /gene="hcxB"
                     /locus_tag="RH058_001772"
     CDS             15519..16604
                     /gene="hcxB"
                     /locus_tag="RH058_001772"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV3137364.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16833..17093)
                     /locus_tag="RH058_001773"
     CDS             complement(16833..17093)
                     /locus_tag="RH058_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3137365.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17358..17624)
                     /gene="ybiI"
                     /locus_tag="RH058_001774"
     CDS             complement(17358..17624)
                     /gene="ybiI"
                     /locus_tag="RH058_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV3137366.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17698..18375)
                     /gene="ybiX"
                     /locus_tag="RH058_001775"
     CDS             complement(17698..18375)
                     /gene="ybiX"
                     /locus_tag="RH058_001775"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV3137367.1"
                     /translation="MMYHIPGVLSPQNVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18417..20699)
                     /locus_tag="RH058_001776"
     CDS             complement(18417..20699)
                     /locus_tag="RH058_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDV3137368.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20964..21224)
                     /locus_tag="RH058_001777"
     CDS             complement(20964..21224)
                     /locus_tag="RH058_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3137369.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21500..22426
                     /gene="rlmF"
                     /locus_tag="RH058_001778"
     CDS             21500..22426
                     /gene="rlmF"
                     /locus_tag="RH058_001778"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV3137370.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22423..24648)
                     /gene="ybiO"
                     /locus_tag="RH058_001779"
     CDS             complement(22423..24648)
                     /gene="ybiO"
                     /locus_tag="RH058_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV3137371.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(24765..25487)
                     /gene="glnQ"
                     /locus_tag="RH058_001780"
     CDS             complement(24765..25487)
                     /gene="glnQ"
                     /locus_tag="RH058_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV3137372.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25484..26143)
                     /gene="glnP"
                     /locus_tag="RH058_001781"
     CDS             complement(25484..26143)
                     /gene="glnP"
                     /locus_tag="RH058_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV3137373.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26282..27028)
                     /gene="glnH"
                     /locus_tag="RH058_001782"
     CDS             complement(26282..27028)
                     /gene="glnH"
                     /locus_tag="RH058_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV3137374.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27432..27935)
                     /gene="dps"
                     /locus_tag="RH058_001783"
                     /gene_synonym="pexB"
     CDS             complement(27432..27935)
                     /gene="dps"
                     /locus_tag="RH058_001783"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV3137375.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(28234..29121)
                     /gene="rhtA"
                     /locus_tag="RH058_001784"
     CDS             complement(28234..29121)
                     /gene="rhtA"
                     /locus_tag="RH058_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV3137376.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29356..29421
                     /locus_tag="RH058_001785"
     CDS             29356..29421
                     /locus_tag="RH058_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137377.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            29474..29989
                     /gene="ompX"
                     /locus_tag="RH058_001786"
     CDS             29474..29989
                     /gene="ompX"
                     /locus_tag="RH058_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV3137378.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(30038..31621)
                     /gene="opgE"
                     /locus_tag="RH058_001787"
     CDS             complement(30038..31621)
                     /gene="opgE"
                     /locus_tag="RH058_001787"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3137379.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(31893..32021)
                     /gene="mntS"
                     /locus_tag="RH058_001788"
     CDS             complement(31893..32021)
                     /gene="mntS"
                     /locus_tag="RH058_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV3137380.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            32207..32674
                     /gene="mntR"
                     /locus_tag="RH058_001789"
     CDS             32207..32674
                     /gene="mntR"
                     /locus_tag="RH058_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV3137381.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            32671..33789
                     /locus_tag="RH058_001790"
     CDS             32671..33789
                     /locus_tag="RH058_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV3137382.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(33848..34768)
                     /gene="ldtB"
                     /locus_tag="RH058_001791"
     CDS             complement(33848..34768)
                     /gene="ldtB"
                     /locus_tag="RH058_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3137383.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            34987..36579
                     /locus_tag="RH058_001792"
     CDS             34987..36579
                     /locus_tag="RH058_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV3137384.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
BASE COUNT         8783 a         9343 c         9750 g         8762 t
ORIGIN      
        1 agcacgaagc caggatatta aacagcatat ggctcattac cgctttaggt ttggtgacaa
       61 ccgcaggact ggcctgggta gcgaagtatc tcgaaattac ggcaacaaaa tatgattcac
      121 cgcaaatgta tgtcgccata ggattattat tactttgtat gtatggctta agtaaggata
      181 tcaacaagat aaatgccgtc atcgcaggcg taatatatct atttttactc tctttggtgg
      241 cgattgtcgt tgctagttta gctcctgtac ctgccattat cgtcgtgttc agcactgcgg
      301 gcgcgatgtt tttaatcagt atgctggccg gtttattatt taatgttgat cctggttctc
      361 accgttttat cattatgatg acgttgacag ggttggccct ggtgatcatc atgaatgccg
      421 cattaatgag tgaacgaccc gtttgggtaa taagttgctt aatgattgtg ttatggtcag
      481 gcattatctc gcatggacga aataagctcc ttgaattggc ggggaaatgc catagtgaag
      541 agttgtggag tccggttcgt tgcgctgtta caggtgcatt aacactctat tactatttta
      601 tcggcttctt tgggatactt gccgcgatag ctataacgct tgtctggcaa aggcatacgc
      661 gtttttttca ttagtcgtaa atgcagatac taaagaactt aactaaactt cacatcgccg
      721 cttcattttt cgcccgtagc ttcttcgcct ggctttccag tagcaggtaa cagatcagcg
      781 ccagcagcag cgggataaag taatacagga cgcgataagc gagcagggcg gcgataatgc
      841 tccctttcga ggtatgctcc ccagccagta gtgcgataaa caccgcttcc agcacaccga
      901 tccccgccgg aatatgcacg atgacgccag caatactact aaccagtaac acgcccagta
      961 caaagaaata gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc
     1021 agttaacgct ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct
     1081 tttgcccttt gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg
     1141 cgataatcat cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt
     1201 gatccggcaa ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca
     1261 gccagttggt ggtaatactg agcgagaaaa tccgcgtaat agtgctgccc ggtaacccta
     1321 gtcgggagta caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt
     1381 tgaaggcgta gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc
     1441 agtaaaagcg ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcc
     1501 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
     1561 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
     1621 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
     1681 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
     1741 ctgcgccaga cgtggtgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
     1801 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttcg gcagcatggt
     1861 ctcatcgacc tgctgacaat ctgcggcaat aatgccgttc agattatcgc gcagcgtctg
     1921 gttaaaatga cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
     1981 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcca ctttgccgtg
     2041 gagcgggcgg cggcggtact caaatacctg aacgccgcct ttaaccagat agttatacag
     2101 caagcgcgca ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
     2161 ccgcaccccg cgccgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
     2221 gtaggcgttg gcgataatga cttctcgccg cgcctgagtg agcattttca gatagtgacg
     2281 ttcaatatca tcgcgatgtt cttcgttatc gcgccagacc agcaatacct gcgcttctcc
     2341 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
     2401 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
     2461 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
     2521 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
     2581 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc gaaataccac
     2641 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
     2701 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
     2761 tttgccgacg tcatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
     2821 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
     2881 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
     2941 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
     3001 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
     3061 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
     3121 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
     3181 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
     3241 tgtacgcaca tcacatgaat cgcttttccg gtcatcggtg gcacaatgcg gcagtagagc
     3301 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
     3361 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
     3421 gcaaaatcgc tccacatcgt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
     3481 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
     3541 gcgctgacgg tacgcaccgc gtcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
     3601 gtaaaacctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
     3661 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
     3721 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
     3781 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaact ggtctaagtt
     3841 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
     3901 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
     3961 tgttggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
     4021 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgtg
     4081 tactggaaaa caactttttg ctggcaattg cagggctggc aatccttgcc ggcccggcgg
     4141 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
     4201 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
     4261 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
     4321 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
     4381 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
     4441 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
     4501 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
     4561 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
     4621 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
     4681 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
     4741 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
     4801 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
     4861 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
     4921 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
     4981 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
     5041 agcagcacat gagtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
     5101 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
     5161 aacgggaaca gaatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgcggt
     5221 tcgcgcagca gcgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
     5281 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
     5341 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
     5401 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
     5461 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcagaaa
     5521 agcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
     5581 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaatcagcag
     5641 cgacccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcaacatcgc
     5701 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
     5761 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
     5821 ggtgagaatc gcgccgatga ccgtcatgat aatggtcacc gcaccgggga taatgaagtg
     5881 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
     5941 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
     6001 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
     6061 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
     6121 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
     6181 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
     6241 cagtaaaatc ccgacccgca gcttgctgga gtcgaggtta atgccgtaac caaaaataaa
     6301 cagcagcaac agcgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
     6361 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
     6421 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
     6481 cgggttgctc gtcgttggcc gactgcgctt tcaaatcatc cggcgtgccg ctggcaatta
     6541 atttcccacg gtacaccagg ccgatgcggt cgcaatattc tgcttcatcc ataaagtggg
     6601 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc cagaattcgc
     6661 ggcgagtaat cggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
     6721 gcatcagcga gcaggccagt gccagccgct gtttaaaacc taatggcagt tcatcggtgg
     6781 cgtgggaggc gatacttttc aggccgaacg cctcgctcat acgggagatt ttttcgttct
     6841 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
     6901 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
     6961 aactctcttt cagatccatc cccagcacca gcgcctggcc ggaagtcggc accagcaaac
     7021 cgcacatcat cttaaaggtg gtcgatttac ccgcgccgtt tggccccagc aaaccaaaaa
     7081 tctccccacg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
     7141 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
     7201 ttgcgcccag cggcgattct gaggttccgg caccacccag caaatcaata aatgcatctt
     7261 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
     7321 cgtctggtgt ggcctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
     7381 cgctgacctg tggcagtttc aaggcgcgtt gcaatagttt gcggttgccc tcgtgtggac
     7441 tggtcatcag aaaactgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
     7501 acagcaactc gccttcgttc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
     7561 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
     7621 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
     7681 gttcgcccac cagggtacag gccagaccga gtttttgttt catcccaccg gagagcttgc
     7741 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
     7801 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
     7861 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
     7921 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
     7981 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
     8041 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
     8101 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
     8161 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
     8221 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
     8281 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatagggctt gtccgggcga
     8341 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagatt ccgttcatca
     8401 acataggcac gtacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
     8461 ttgagaaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
     8521 aacgttgagt cctgcaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
     8581 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
     8641 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
     8701 tcgtttgccg aaatggtgcg gcttttccac aacccttgct ggcggttata gaagttctgc
     8761 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
     8821 tcgcgatacc cggcaagcat caggtcatac tgcgcctgtg ccaccgagac gcccgctttc
     8881 gcctgcatca gggcaatctc atatggcttg tgatccagtt cgcccagcac ctggcccgct
     8941 ttgatcgcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
     9001 agatttaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
     9061 tgataccacc agtagcctcc ggcaaccacg gcggcaagtg ccactaccgc caatccgatc
     9121 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
     9181 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
     9241 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
     9301 catgagtatg gaggatcatg cgggtatcgt tggcgtcgca gccggtccag gcggcaatca
     9361 ggcgtgtcag gtggctgtgt agcgggctaa tcacctgttc gtgcaccagg tgataggctg
     9421 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
     9481 tgagcagctt aatcatgttc ctgcaggcgc gaaggatcag ttcacggatg gctgcgcgat
     9541 caggctgtgg ttgtgcgaac aagcgttcgg cctcttcggc gtgcggacgg aactgctcgc
     9601 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
     9661 agtaggtgat ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtagtggcgt
     9721 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
     9781 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
     9841 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
     9901 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct ccctcatgac
     9961 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    10021 ggggcggttc ggagtagtta tgtctttcga ttctttgggt ttaagccctg atatcctgcg
    10081 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    10141 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggtaccggta aaacagcggg
    10201 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    10261 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gctgcgcaga ttggcgaaaa
    10321 cgtccgtgat tacagcaaat atttgaacat tcgttcgctg gtggtgtttg gcggtgtcag
    10381 cattaacccg caaatgatga agctgcgcgg cggcgttgat gttctggtgg caacaccggg
    10441 acgtttgctg gacctggaac atcaaaatgc agtgaagctg gatcaggttg aaatcctcgt
    10501 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    10561 aacaaaactg ccagcgaagc gccagaacct gctgttctct gcgaccttct ctgacgatat
    10621 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    10681 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    10741 attgctgtcg cacatgattg gtaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    10801 caaacacggc gctaaccatc tggctgagca gctcaataaa gatggcatcc gtagtgcggc
    10861 gatccacggg aataaatcgc aaggtgcgcg tacccgtgcg ctggctgatt ttaaatcggg
    10921 tgacattcgc gtactggtgg cgaccgatat cgctgcgcgc ggtctggata tcgaagagtt
    10981 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc accgtatcgg
    11041 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    11101 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    11161 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    11221 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    11281 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    11341 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ctgctgcgca
    11401 gtaatcttta atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    11461 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatactttt
    11521 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    11581 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    11641 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    11701 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    11761 gcgcggttgc cagcaagagc gcacgcagtt ctgcatgctg ttcgaattta gcgcgaagtg
    11821 cttttcgcat cacctgttct ttgaccgact cccagttttt acgcagaggc ttagaacgat
    11881 cgcgccccat gcgtgcggcg accatagggg aagaaacccg acggatctct tcgcggtatt
    11941 tttcatcaag gaatttttgt gcctgaaaat agtgttctga ggtgggccag gttttcccgt
    12001 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    12061 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    12121 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    12181 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    12241 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    12301 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    12361 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    12421 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    12481 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    12541 aagatcattc ccgatcttaa attcactgcc gcttttgggc gtgggcgcta cgtttgcccg
    12601 cgtaatctga cggcgctcgc cagcactgag cccacgcaac aggatctgct ggcgtttctt
    12661 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    12721 gatctcgaca cttataaatg ggatggtctg cgcgatcata ctgatatcgc tatagatgac
    12781 gatctctggc gtcgtttaag taccgacaaa gccagctgcc tcaaccgcaa ctgttactac
    12841 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    12901 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactacc tgacccgaaa
    12961 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    13021 gagatgagtg ccgaaatcac cgcgccttgg tatcgactac agctggactt gttcacgaaa
    13081 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    13141 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    13201 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    13261 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    13321 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    13381 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    13441 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    13501 cgggaagagc gcgaagggca gctgcatctc tggtttcact gcgtgggaat acgtgtcagc
    13561 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    13621 ttgcgttcgc tgaacagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    13681 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    13741 attccccgga tgcgctttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    13801 gcggcctttt tccgtgagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    13861 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgctg
    13921 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    13981 aacggtgagc atagtgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    14041 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    14101 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    14161 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    14221 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    14281 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    14341 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    14401 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    14461 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgcccatat gctcaatggt
    14521 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tacgtatcaa aggggaaggg
    14581 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    14641 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    14701 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    14761 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    14821 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    14881 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    14941 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgag
    15001 gcgctgcgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    15061 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    15121 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    15181 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    15241 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    15301 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    15361 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    15421 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    15481 aaaaaacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    15541 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    15601 aagaagcgaa attagtggcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    15661 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    15721 accatcatgc caaaaccgtt aaagagtcgg gggcggcagt cacgctcgat ggcgatcgcg
    15781 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    15841 acggtattgc tgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    15901 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    15961 caatggtcgc accgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    16021 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    16081 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    16141 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggggcgc
    16201 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    16261 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    16321 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    16381 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
    16441 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    16501 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    16561 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    16621 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    16681 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    16741 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    16801 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    16861 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    16921 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    16981 ccgattttat tcatgttctg tgcctgggat gccgttaccg gttccgcggc aaacacgcca
    17041 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    17101 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    17161 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    17221 tctaacatac tgataaacca tttatttgtt gtacttatgg acttgtttta tgccgcgtgg
    17281 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa tacaaattat actcgctatg
    17341 catctcgcca gttgtagtta acgtaactgg ctatctttcg aacctctgcg attatatcct
    17401 gtatacgctg gtttttgtaa atctttctcc tgctgacaat gaatgcataa gcgcacgcca
    17461 ggaatggctt cccgacgggc ttgcgggatg ggggcaccgc actcttcaca ttcatccagg
    17521 ctttcgccac gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    17581 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgtc ctccccatat
    17641 caatgcttgt acagttaagt gtagctaatc cagggacgaa cttgggcagt tcaagcatca
    17701 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    17761 attttcacca tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    17821 gcgcttttta tcatcacgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    17881 gcgggttacg ggtgtcacgc aatgcaggct gctggaggga tacaacacga gatcgcctgc
    17941 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    18001 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    18061 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    18121 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaata aagcatgttg
    18181 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    18241 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    18301 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgttc tgtggcgata acacgccggg
    18361 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    18421 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    18481 ctcttgttga ttgaggcgac gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag
    18541 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    18601 aacgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    18661 cccgcgccaa cagagatatc atctgttgcc tgatactggc tccataaggt gaaggcgtgc
    18721 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    18781 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggcgtgat attcccggcc
    18841 acggatatct catagccttc gacacgtttc ttaccgtatt gcgagtaagt tccgtcatca
    18901 ttttgctcaa cttcattttc gatatcagta cggaatagcg cggcggtgag caacaggcgt
    18961 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    19021 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    19081 ggaggctgct gggaaacggc atagttaata tagacattac cgttttccgt caggtgatac
    19141 agcgccccgg ctttccagtt caccagattg cccgacttgg cggtgtcgac ggtggtgacc
    19201 ggagaacctt ttgccacacc agctgggcag gtgatggcac cgcgtccgct gccgccgcag
    19261 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    19321 aaatcacggg tgatttgcag cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca
    19381 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    19441 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    19501 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    19561 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    19621 gtccagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    19681 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    19741 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    19801 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag agccgctgtt
    19861 cccgcagatg gggcagaata acccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    19921 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    19981 gcgatagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    20041 gttttttcgc ccatcacatt caggcgcacc gcagtggtat caccaatgac ctgattgacg
    20101 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    20161 tcattgcgcg gctgcttgct gatcatattg atcgagcccg tcggcgcgct gcgcccgtag
    20221 tcggtgccgg aaggcccttt aatcacttcg acctgctcag tattgaaggt gtcgcgcgag
    20281 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc agcaccgcgc
    20341 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    20401 ccggggacgt ttttgagcgc gtcggtaagg ttggttgcgc cctgatcttt aatcacttgt
    20461 tcagaaatca ccgtcatcgt gcgggtagta tccgctaccg gacgcgagaa tttcggatcg
    20521 gcagattgtt gtggcgcata aagcgaaggc gttgatgctt cgacaaccag cgtgtcatcc
    20581 gcgttagttt gcccttcggc ggcgagtgcc tgagccacag gcgtgatgcc aatacaaaga
    20641 ccggcaaaga acgtgagcga atgaaattgt ctggcaggga aattgcgatt gttttccatt
    20701 ttgcaggtga ctttttttta tatgttgaaa ttttcactgc cacgagaaag cctctctgcg
    20761 gatgcgatgc cactgttaat ctcgtgatag tgttcgtgca agtgataata attttgataa
    20821 gcattatcac ttggcgcgat tttctatcac ccattgcgcg aaaaagaaat acatttattt
    20881 acattgcgtt catatttgat gttttttgat tacaaaacag gcgaaaactg catccagagg
    20941 cgaatgcagt tacgttggtg gggttatttg tagatggttg cagtaccgaa catctggtca
    21001 tttccgcctg cggaattaat gacataacct ttcgcgcctt gctcgcgcgc tttttctgcc
    21061 agtttatcct ccagatcgct taggtttgat gcgccagttg ccgaaacggt accggcgggc
    21121 cgtagctgac cgctgtccag gttactcatc ggttgcgcgg cataagccgt gagagagatt
    21181 ccgctcagga cggtggcaat cagtagtgtg aggcactttt tcataatgac atcctcataa
    21241 atacaacggg tagtgcctga taagtgtagg tcgccagggc gatgaaaatg acgcaaaaaa
    21301 ttgacagaga tgtgcttatt ttttgaacga tatattttta caaaataaga caaatcaatg
    21361 acatgcataa tgctgatttt tatgatgaat tgccataaac catagccatg ttaaggtttt
    21421 ggggaatgat aaacgcagcc agatatttac cctgtcgcgt tatgcgggta tcttacgccg
    21481 ctgcttaaag gagaatgcta tgtccgccca gaaaccgggg ttgcatccgc gcaaccgtca
    21541 tcacagccgc tacgatctcg ccacgctttg tcaggtcaat cctgaactca ggcaattcct
    21601 cacgcttaca cccgcagggg agcaaagcgt agactttgcc aatccgctgg cggtgaaggc
    21661 gctcaataag gcattgctgg cccattttta cgccgtagcg aactgggata tcccggacgg
    21721 ttttctctgc ccgccagtac cgggccgggc ggattatatt catcaccttg ccgatttact
    21781 ggcagaagca agcggaacaa ttccagctaa tgccagcatt ctggatatcg gcgttggtgc
    21841 gaactgtatt tatccgctga ttggcgtaca tgaatatggc tggcgtttta ccggtagtga
    21901 aaccagcagt caggcgttaa gtagtgcgca ggcgatcatc agtgctaatc cggggcttaa
    21961 ccgcgccatt cgtctgcgtc ggcaaaaaga gagtggggcg atttttaacg gcatcatcca
    22021 taaaaacgag caatacgacg cgaccttgtg taacccgcca ttccacgatt ccgccgctgc
    22081 ggcacgggca ggtagtgagc gtaaacgccg taacctgggg ctgaacaaag acgatgcact
    22141 gaactttggc ggccagcaac aggagttgtg gtgtgaaggt ggtgaagtcg cctttatcaa
    22201 aaagatgatt gaagagagca aaggcttcgc gaagcaggtg atgtggttta cctcgctggt
    22261 ttctcgtggt gaaaacttac cgccgttgta tcgtgctctg acggacgtgg gcgcggtgaa
    22321 ggtggttaaa aaagagatgg cccaggggca aaagcagagt cgctttattg cctggacctt
    22381 tatgaacgac gagcagcgcc gccgttttgt taatcgccaa cgttaaagcg ttggctcccc
    22441 cggcggcagc ggttcagccg gggccgcgcc cggagcaggc agcacctgat aagtctgcac
    22501 tggcgcgcga acacctgcca ggtcgaaatg ctttttcacc tggctgtcga gggcaaagcg
    22561 gaccgtccac tgtttgagtg gcagcgtggt gaacgaaaca cgcagtgtaa acgcggtatt
    22621 gcttaagccg acaatcccgg caaagtttgg ttcaccaata accagcccgc gaatttcttc
    22681 gttttccatc aattccgcta ccgcatcttt cagtgcctga ttggctttat cagcatcttc
    22741 atggcgatca acatcgtagt ttgccaccac cgaaccaatg ccgcggacga agttagcaaa
    22801 ggtggttatc gaagaccaag gaatgatgtg atacgccccg gtatcctgac gcacgcccac
    22861 ggagcgaatc gacatccgtt ccacagtgcc ggtcaacggc ccgatagtaa ccaaatctcc
    22921 agtgttcatg ccgttttcaa actgaataaa taccccggtg ataatatctt tcaccagcgt
    22981 ttgcgaacca aacgagatag ccagacctaa tgccccagca cctgccagca atggcgcgat
    23041 attgacgccg atttccgaca acacaatcat gatggtgatg gtactgataa tcaccgccag
    23101 cgcgttacgg aacagcgtca gcagggtacg cgtacgggcg ctgggtagcg ggcggccatg
    23161 aatatccgaa gccagccggt tttcgatcaa actggcgagc actgtccagc caaccgccga
    23221 gaagaaaaga atgagtgcga tgcggatcag gatatctacg gttttctgcc ccgcgccgtt
    23281 ttgcagccag ttccagaaat cgaacaatcc ccatgcgctc aacagcaaca ttaccgccac
    23341 gcagacggtc agaatacgcg ccgttttcag cgccgccgaa agccagccat tcagccgttt
    23401 ttgtagctcc ggatagttac gctgagtatg tggcgagagg gtgatggttt tcgccagcca
    23461 gcgggaaaac ataccggaaa caaacgctgc gataccaata atcgccaggc tgcgcaccgt
    23521 tgcacccatc ataaatttca ggctgttgcc cggatcgaac aacgaaaaga aaaacagcac
    23581 gataaaatag gcgcttgcca gccagtgcca caccagcgca aaggcgcgga taaacaggct
    23641 gaaaaaggcc agcgaatgct ccgcaaagtt gagcaaatgt tgggtaatct cttttttatt
    23701 gcgaaagatc aggtacaacg cccagacggt catgcacagc ataatgatga cgttcgccag
    23761 cgcacctatc tgtacattca cctgattaga gataatcggc acggccacaa tcaggccata
    23821 acctatcaaa ctgcttaacc agctcaggcg acgactccag taacgggcgc tctcgtcctg
    23881 aatcgtgaac gggcgcagtt cagccacgtt tgggcaaaaa atcaggcgta gtacggcttt
    23941 gaaaaattca atcagggcaa aggcgttgag aaacaaactt tgttggaaag cgatggtgcg
    24001 actgcccgca ttcaggttgt cgcttaatac ctggccgaca aacaatgtca gtgccagtaa
    24061 cagtaggtcg ataataaacg ccccgataat catcgccgga agctgcagcc agttgctgcg
    24121 ttcgcgattt ttttgccgcg cccactggcc cattttgcga tacagcggca gtgcgcacag
    24181 acgaatcagc cagtaaaaac caaacactaa taccgctaac attgaaaaat gggtcaacgc
    24241 attgctgaag gtttgtggat taaacggctt atgcggggag ccggtgatat tgcgataaag
    24301 ttgtccgaag cgggcggaaa gggcttcacc ataatggcgg ctgacttcgg tgaccttttg
    24361 cagcacggtt tgctcttcaa ccagcgtcgg cggcacgatc ttcgggaccg gttccgcagg
    24421 gggcgtcgcg gcgacagtac gcaactggtc gatcaattct ttacgcgagg tgtcgttatc
    24481 cagcacatcc gccagtgcgc cataggccgc ttttttttgt tcgatatccg gttccggggc
    24541 cggttcagtc gttgagtccg ttgtcgttgt ggttgtaacg ccgggtatgg ataccgcgtg
    24601 ggcaggtgcg cccagcaggc agaagaggat gaacaggatc caccgcatga ctcctccagt
    24661 gagaaaacag agcaaaaaga taagtataga tgctggggga ggggcgattt taaatgagag
    24721 gaatctggtg tgcctccctt tcgggtgaaa gggaggcaga gaagttaaga gacgtgctgc
    24781 aaaaattcct gcaagcgctg gctcggcggg ttcttgatca atacctgcgg atcgccatct
    24841 tccgcaatcc ggcctttgtc gataaagatc agccgcgaag ctactttctc ggcaaaaccg
    24901 atttcgtggg tcacgatcac catcgtcatc ccttcttcag ccagatcctg cataaccttc
    24961 agcacttcat ggcgcagttc cgggtcaaga gcggaagtcg gttcatcaaa cagcatcatt
    25021 ttcggcttca ccgccagcgc gcgggcaatc gccacacgct gctgttgacc accagaaagt
    25081 tcggaagggt agtgatgtgc acgttctgcc agaccgactt tcgccagcag ctcacgcgcc
    25141 agtttttccg cctcttcttt gttcgcgcca cgcacgcgta gcggaccaaa catgacgttt
    25201 tccagcgccg tcagatgcgg gaagaggtaa aactgctgga acaccatacc tgcttcctgg
    25261 cgaatcaggc gctcgtcaac tttcggatcg ttaaccttca ggccatcgac aatcagatcg
    25321 ccggaggtga tttcttccag tttgttgatg cagcgcagca gggtcgattt accggaaccg
    25381 gacggcccga taatcaccac gacttcgccc tgggcaatgt tcaaatcgat attgtgcagc
    25441 acctgggttg ggccaaagtg cttggagacg tttttaaatt caatcacagg attttcatcc
    25501 ttctttccag acgacgcaga ataaagctca gcaccagggt aataatcaga tagaacaccg
    25561 ccacggcgct ccagatctca agggcgcgga agttaccggc aataatttct tgcccctgac
    25621 gggtcagttc cgccacgccg atcacaataa acagcgaggt gtctttaatg ctgatgatcc
    25681 actggttacc cagcggcggc agcatacgac gcagtgccag cggtaaaatg acgtagcgaa
    25741 tggtttccca acgtgaaaga ccgagcgcca gtcctgcttc acgaaaacct ttgtggatag
    25801 acagcaccgc accacgcgtg atttccgcaa tgtacgcgcc ggagttgatc atgatggtga
    25861 ccaccgccgc agtaaatggg tcgatgcgta agtcgttaaa cgccatcggc agggcgaaat
    25921 agataaacat cacctggacg acgataggtg tgccgcggat cacttcaata aagaccagcg
    25981 cgacgtggtt ggctatccaa ccaccgaagg tgcgtgcaaa acctgccagc aatccgatta
    26041 ccagaccgcc tgccagaccg aggaccgaaa tccacagggt cattttggca ccttcaatca
    26101 gaagcggaat ggcaggccag atggcactcc agtcaaactg catatgttgt tcctgttacc
    26161 gtggtgtaaa taatcaaatt cagggtaatt aatggccccg gagcgggttt gcgcccctca
    26221 ccctaatcct ctccccagag gggagaggga actgccagtg cgttttacag gtgtagcgtt
    26281 attatttcgg ttcagtaccg aaccattttt tgtagatttc gttgtaagtt ccgttctcgc
    26341 gcagggtttt caacgcgccg ttgactttgt cacgcagctc gtcgctacct ttcgggaacg
    26401 caataccgta ttgctgcgct tccagagagt cacctaccgc tttgaactga ccgttaccgg
    26461 cggttttgat gaagtacaga atgtttggcg tatcgtgcag aacggcgtct gcgcggttgg
    26521 tgcccagttc catataggcg ttatcgatgt tcgggaactg acgcagatct ttagttttga
    26581 tgtttgcttt cgcgtaatca acggagccag taccgctctt cacagcaacc actttcccgt
    26641 cgagatcttt cacgcttttc acatcgttat tgttagcttt caccatcact aacaggccgc
    26701 ttttgtagta gccgtcagag aaatcgatcg cttttttacg ctcgtcggtg atggtaatgc
    26761 ccgccagcgc cagatcgacg tttttggttt gcagtgccgg aatgatccca ctgaaatcca
    26821 tcggcttcag ttcgtaatcc agcttcagct ctttagcgat ggcagcccac agatcaacgt
    26881 caaagcccac atatttatcg ccctgtttaa attcaaacgg aacgaaggcg gtatccgtcg
    26941 cgacaactaa ttttttatcc gcggcatgag aagaaaccgc aaaagccagg gtcagtgcag
    27001 ccagtgaaac ttttaataca gacttcatag catttccttt tttctatcca cgggacgatc
    27061 ccctgcgtga cattcacata tatgaaaaaa tcgtgccagt tttgcaactc cttgattttt
    27121 cgagatgcgg caaaaacgtg atgcacgatt tatagggcaa taccctgaag atgcaccatt
    27181 ctggggcacc aatctggtgc gctaaaattg tgcactcaac acagtgatta tttagcgtaa
    27241 aacctgatga aaaaacaatc tttatgtaac gattgtgtga tgatgtggat acaaaaaatt
    27301 tacttaatca gctggagata gcagatggat gcactaaata agtgcgttga ggtgggctgc
    27361 aaaacaaaac ggcctcctgt caggaagccg cttttatcgg gtactaaagt tctgcaccat
    27421 cagcgatgga tttattcgat gttagactcg ataaaccaca ggaatttatc caggtcgcga
    27481 gacgcggcgg tcaggatatc tgcggtgtcg tcatctttcg cttcgccaat cgctttgcgt
    27541 acgtcattag cgacgattgc gtaacggtca gccagttctt tcaggtgatc ctgaacgttg
    27601 tggatgtcca gcgggtaact tttcagcggg gttttgctgt tgataacttg agtggtcccc
    27661 agagctacac cgcccagctg cactgcacgt tctgccatgg tatccagatg atcgatcagt
    27721 gcggtgcgga agccatccag catttcatgt acggcaatga agttagcgcc gcgcatgttc
    27781 cagtgcgctt gtttggtaat caaagaaaga tcaataaact ggataacctg gcgattcagc
    27841 aactctactg ttgctttttt ctcgctgtcg gagacatcgt tgcgggtata aagcagattg
    27901 gtcgcttttg atttaactaa tttagcggta ctcataattt catatcctct tgatgttatg
    27961 tcccagtaat taacgagact aagtatagca ccggctatgt gttccgctat tctggctgtt
    28021 cctatcacac taatagtggt aacaagcgtg aaaaacaaaa ctaataaaga ttcaatgagt
    28081 tagatatatt gataagaaca attcttattt atctgctcgc caggaatttt cggaataaat
    28141 atgcttttgc ataattcgat atataaagac ggtgtagagg aaaagtagcg agaaattctg
    28201 catggttatg cataaccatg cagaaatgta aatttaatta atgtctaatt cttttatttt
    28261 gctctctttg cgtactgtca gcgtagaccc cattgaagcg gcgatgatag cgccgagcgc
    28321 cagcagctgt atgggtgtca gtgtttctcc gaggaaaatc atcccggaaa cggcagccag
    28381 cgccggttcc atgctcatca gcgtaccaaa tgtccgtgtt ggcaaacggg tgagggcaat
    28441 catttccagc gaataaggca gagcggtcga gagaatagcg acagccagac ccaatggaat
    28501 aaccgaccag tgccagagtg cttcaccagc ctgaagcgct ccaattggca cgaaaattaa
    28561 cgctgcaatc aacgaaccaa ttgccaccgt cgcagggcca tgttccgctc ctgcgcgttg
    28621 cccacttaaa atgtaaatag cccaacaagc cccggccccc agtgccagcg cacagccggt
    28681 taaatcgaca tgggaaacgt cttgccccag cggtagcagg aaccacagac caagaaccgc
    28741 cagcacaacc cagacgaaat ctaccgggcg acgagaagag aacagcgcca ccgccagtgg
    28801 tccggtgaac tccagcgcca ccgcaatacc cagcggtact gtctgaatag aaagataaaa
    28861 aagataattc atcccaccca gcgaaacgcc gtaaaacaac agcggtaacc gttgctcttt
    28921 ggcaaagcgc agtcgccatg gcttaaagaa cgcgatgagg atcagcgttc ctaatgccag
    28981 acgcagcgca gtgacacccg gtgcgcccac cagaggaaaa agtgacttag ctaacgaggc
    29041 tccaccctga atagacgcca tggcaacgag caatatgatt attggtaacc agaccggcat
    29101 tttacgtaat gaaccaggca tcctttctcc cacaaatatc tagacttaag taaagcgtgg
    29161 agtgtactgg atatacccaa tgctggttga gcatttgttg aaaaaatttt cccccgtttt
    29221 gactaaaatg cgccaggatt gatggaatca ttagtctggt gattaggaat aatctggatg
    29281 aatgacaggg aaaacatgcg taatacttac gcagttctct gaaaaagtga tttaaattta
    29341 gatggatagc ggtgtatgga aacgttctgt tacatgaaat ggcccgttag acatcacaaa
    29401 tcgcgaagag tttcccatta atttttgata tatttaaaac ttaggactta tttgaatcac
    29461 atttgaggtg gttatgaaaa aaattgcatg tctttcagca ctggccgcag ttctggcttt
    29521 caccgcaggt acttccgtag ctgcgacttc taccgtaact ggcggttacg cacagagcga
    29581 cgctcagggc caaatgaaca aaatgggcgg tttcaacctg aaataccgct atgaagaaga
    29641 caacagcccg ctgggtgtga tcggttcttt cacttacacc gagaaaagcc gtactgcaag
    29701 ctctggtgac tacaacaaaa accagtacta cggcatcact gctggtccgg cttaccgcat
    29761 taacgactgg gcaagcatct acggtgtagt gggtgtgggt tatggtaaat tccagaccac
    29821 tgaatacccg acctacaaac acgacaccag cgactacggt ttctcctacg gtgctggtct
    29881 gcagttcaac ccgatggaaa acgttgctct ggacttctct tacgagcaga gccgtattcg
    29941 tagcgttgac gtaggcacct ggattgccgg tgttggttac cgcttctaat cactttggtg
    30001 atataaaaaa tccgcctctc ggggcggatt tttgttttta aggcttcggg tcgaaaatat
    30061 cggttccgag atggttgtaa tccaccttct gtaactggaa gttggtgatg tatatcgacc
    30121 cggctttctt ctcagatata aacgggtatt tgatgttaat ttccttcgct ttaattcccg
    30181 tccactggga gaaaaaacct aagaagtcat tggctgagcg gcgggcttta atcacccgat
    30241 gcgctttatc gtcgctggaa atgaccataa aaggcacctg gaaattttgc tgatacttat
    30301 catcatgggc aaggtattgc acgtctttgc cgcgctcttt aaaggccaga ccgtggtcag
    30361 aaaagtaaac cagcgagaag ctgctgccgc tgttgcgtaa ctgatcgtac agcttgcgca
    30421 gtaaatcgtc cgtttgcgtc atggtataga gatagcacga cgtttctttc gactgcacaa
    30481 aggtttcgta tttgccttgt gtcctgtcgc aggcctgcgg atgtgagccc atcagatgta
    30541 gaacaatcag ctgcggttgc gagtgctctt gcgccagcac ttgagcggtc atatccagta
    30601 acgcttcgtc tttggtattt ttatctgctt caaaattacc ttctttcagg aagtacactt
    30661 catctgctcg tttggcgatg ctggcgatag cagtatcgta ttcgccgatt tggccctggt
    30721 tggaaaacca ccaggtctgg aagcccgcgc gatttgccag ggtgacaaag ttatcctgaa
    30781 actgtggttt gccatcgact acgcgattga gcgttaagcc aagcgatttc tgcgtggagc
    30841 cactggcggc aatgtagtca gcaaatatca aaccgttaac gctgctggca aacggggtat
    30901 tgtcccagtg accgccaaag gcaccgaggg catcgcgacg cgcgctttca ccgatcacca
    30961 ccacataggt ctgatacttc ggcttaacgg cagtgaccgt ccaggtatct ttcattccgg
    31021 aaagttttgc catccgttct tgctcttcaa tcacctcgtt attattgacg atgacatcct
    31081 tagcaaaacg aaataccgga tagccagtat ctttcagttt aaatacgccg ccccaggcca
    31141 ggttttgcac gggagcaaca aaaaatgtcg ccacactgaa taccagacac aggctgtcga
    31201 aggtattcca gcgcgctttt tcactctctt tttttcgcct gattgctatt acgccgagca
    31261 caaaaataaa tatgccgacc atgtagctgt accacggaaa aatcgtcagg atttcggtcg
    31321 actcttccat attggtggag tgcaatgcca gcaatgtatt gaagttaggc gcgccgtagg
    31381 cctgagcaaa agggaaataa caagccgcca ccagcgaact gataccgacc agaacttttt
    31441 gtacgcgagg caatgttcgc catagcaaaa gcaaaatagc cgtaaacgca gcggtgtaga
    31501 gcaaactgaa ggggtaacct aagccgaggt taattaatag cgactgtaaa aagtagaacg
    31561 cagtccacgg gctaaatacc cggctacggg taacaagcga ttctttgagg gttaaattca
    31621 tatgccacta tcgtaacaaa cgccatgtgc ttaccctggc gtcaagggtc aatacctgca
    31681 agagtgcgtg aagaggggaa aggggtccgc atccgcgagc cgcaatatac gcagggctgc
    31741 aagaagatag agcgagccgc cgttcaggtc aactactgac gaacaaatct tttgcgaagg
    31801 ggattgcaaa tccgacaaaa agtggggttt ttcagaccaa aacgcgttat gcctgcatga
    31861 aagcaggagt gaagcggcgt accgcgacgc cgctatttat cggaaggttt atcttgctgc
    31921 ggtttgttgt tgaccatatc gcacaacata gagagcagca ttaaccgtac tttaaaggga
    31981 gaatgactaa acacgcgcat acacctcttg aactcattca taagacctcc tgacttgcta
    32041 atcccgtcga tccttgaggg atgattgcat tacatacaga tatagcacag gctatattat
    32101 atagctattg ctaaaacgtt aattttttgt accttcgcaa ctctggttta caatgtgcgc
    32161 acgaaatgag aacgctatgt atgcgtcacc ataatgagga agcacaatga gtcgtcgcgc
    32221 aggtacgcca acagcaaaaa aagtgacgca gttagtgaac gtggaagagc acgttgaagg
    32281 gttccgccag gtcagagagg cgcatcggcg cgagcttatt gatgattacg ttgagctgat
    32341 ttctgacttg atcagggaag tgggggaagc tcgtcaggtg gacatggctg ctcgtctggg
    32401 agtttcgcaa ccgacggtgg ctaaaatgct taagcggctg gcaaccatgg ggctgattga
    32461 aatgatcccc tggcgtggcg tgtttttaac ggcagaagga gagaagctgg cgcaggaaag
    32521 ccgcgagcga catcagatag tcgaaaattt cttgttggtg ttgggcgtca gtccggaaat
    32581 cgcccgtcgc gacgcggaag gcatggagca ccatgttagt gaagagacgc tcgacgcttt
    32641 tcgtttgttt acccagaaac acggtgccaa atgagcctgc cttttttacg cacgctgcaa
    32701 ggcgatcgtt tttttcagtt attaattctt gttggtatcg gattaagctt tttcgtgccc
    32761 tttgcaccga aatcctggcc tgctgctatc gactggcaca ccatcatcac cttaagcggc
    32821 ctgatgctgc tgaccaaagg tgtggagtta agcggttatt ttgatgtgct ggggcgcaaa
    32881 atggtgcgcc gctttgctac ggagcgtcgg ctggcgatgt ttatggtgct ggcggcggcg
    32941 ctgctttcta cctttctgac caacgatgtc gcgctgttta ttgttgttcc gctgactatc
    33001 acgctaaaaa gactgtgtga gatcccggtt aatcggctga ttatttttga ggcgctggca
    33061 gtcaacgctg gttcgctact gacgccaatt ggcaacccgc aaaatattct tatctgggga
    33121 cgttctggtc tttcgtttgc cggatttatt gcccaaatgg caccgctggc tggcgcaatg
    33181 atgctgacgc tcctgctcct gtgctggtgt tgtttccctg gaaaggcgct gcaataccat
    33241 acgggggtgc aaacaccgga gtggaaaccg cggctggtgt ggagttgtct ggggctgtat
    33301 atcgtctttc tgacggcgct ggagttcaaa caagagctgt ggggactggt gattgtggcg
    33361 gcaggctttg cgctgctggc acgtcgcgtg gtgctcagtg tggactggac gctgctgctg
    33421 gtgtttatgg cgatgtttat cgacgtccat ttactgaccc agcttccagc gttgcaaggc
    33481 gtgttgggta acgtgagtca tctatctgaa cccgggttat ggttaacggc aatcggttta
    33541 tcgcaggtga tcagtaacgt gccgagtacc atattgttgc tgaactatgt gccgccgtct
    33601 ttattactgg tatgggcggt aaacgtaggt ggctttgggt tattacccgg atcgctggca
    33661 aatttgattg cgctacgtat ggcgaacgat cgccgcatct ggtggcgttt ccatctctat
    33721 tcaataccga tgctgttgtg ggcggcgttg gtgggatatg ttttgttagt tatactcccg
    33781 gccaactagg gtctggcaaa taaaaaaggc ggattatgag tccgcctttt gctttaagtt
    33841 ttgaagatta attcagacga accggcatcc cggagcggtt tttaatcgct tcatcaagaa
    33901 caacctggtc aacatctggc tgaccggtca ctgtttgcac gctcttcgtc agggtaattg
    33961 gcacaatttc ctgaccttca aactgggctt cggtggtaga cagcgggtta tggacttcaa
    34021 tataacggct gccgtctggc tcggtggtcg cttttaccgg ctcatcaata aactgtacgc
    34081 gggtaccgac cggtactttc tcgaacagga atttgatgtc ttcgttacgc agacgcacac
    34141 aaccatgact tacacgcagg ccgataccga agttggcgtt ggtgccatgg atagcgtaca
    34201 ggcgaccgat gtagagtgca tacagcccca tcgggttatc cggacctgcc ggaacgacag
    34261 ccggaagcgg ctcgcccgca gcgcggtact ctgcgtgcat tttggcggtc ggcgtccagg
    34321 tcgggcctgc tttcttacgc tcaactttgg tggtccagtt gataggcgta tctttgccta
    34381 actgaccaat gccgatcggc agcacgataa cggtgttggt ccctttcgga tagtaataca
    34441 gacgcatctc tgcactgtta atgacgatgc cttcatgaac ggtatccggc aggatcagct
    34501 gctgcggaat gttcagtaca gtaccgcctt tcggcaggaa ggtatccaca cccgggttcg
    34561 cttccatcat attggaaagc cccatctggt actccgcggc aaaatactcc agcggctgag
    34621 tgttaccttc aggaatggtg atcacctgat tctgaccaac caggcgactc ccgtcggttg
    34681 gcagaggata agttaccgca gaggcggtac tgcaaaagcc gacaacagcg aaggccgctg
    34741 cgaataatgt tttcaatttc atattcatgt taagcgagat tttgtgcctg gcaggccatt
    34801 gggttgagaa tattagagta ttggaagcgc attataagtt cattccagct cacagtgaaa
    34861 tcagatgtgt acgaaatcac attttttgcc tttggcttga gtgtagacct taagcgagga
    34921 gcaggatctt ctttcagact tatggcataa tgcgcggttt gtcatatctc ttttcaggat
    34981 acgcctgtgt tagtttccag taacgtcacc atgcagttcg gcagtaagcc gttgtttgaa
    35041 aacatttccg tcaaattcgg cggcggcaac cgttacggcc tgattggcgc gaacggtagt
    35101 ggtaaatcca cctttatgaa gattctcggc ggcgaccttg agccgacgct gggtaacgtt
    35161 tccctcgatc ccaacgagcg cattggtaag ctgcgtcagg atcagtttgc ctttgaagag
    35221 ttcactgtgc tggatacggt gatcatgggg cataaagagt tgtgggaagt gaagcaggag
    35281 cgcgaccgca tctatgcttt gccggaaatg agtgaagaag acggctataa agtggccgat
    35341 ctggaagtga aatacggcga aatggacggt tactctgcgg aagctcgcgc cggtgaactg
    35401 ttgcttggcg tgggaattcc agtggaacag cactacggcc cgatgagtga agttgctcct
    35461 ggctggaagc tgcgtgtgct tctggcgcag gcgctgtttg ctgatccgga tattctcctg
    35521 ctcgacgaac cgaccaacaa cctcgacatc gacaccattc gctggctgga acaggtgctg
    35581 aacgagcgtg acagcaccat gatcatcatc tcgcacgacc gtcacttcct taacatggtc
    35641 tgtacccaca tggcggatct ggattacggc gagctgcgcg tttatccggg taactacgat
    35701 gagtacatga cggcggcgac ccaggcgcgt gaacgtctgc tggccgataa cgccaagaag
    35761 aaagcgcaga ttgctgagtt gcaatctttc gttagccgct ttagcgccaa cgcctcgaaa
    35821 tctcgccagg caacttcgcg cgcgcgccag attgataaaa tcaaactgga agaggtgaaa
    35881 gcctccagcc gtcagaaccc gttcatccgt tttgaacagg ataagaaact gttccgtaac
    35941 gcgctggaag tggaaggtct gaccaaaggg tttgataacg gtccactgtt taaaaatctc
    36001 aacctgctgc tggaggtcgg tgaaaaactt gcggtactgg gtaccaacgg cgtcggtaaa
    36061 tcaacgctgc tgaaaacgct ggtgggcgat ctgcaaccgg acagcggcac cgtaaaatgg
    36121 tctgaaaacg cgcgcattgg ttactacgcc caggaccacg aatatgagtt tgaaaatgat
    36181 ctgaccgtgt tcgaatggat gagtcagtgg aagcaggaag gcgatgacga gcaggcggta
    36241 cgcagcattc tcggtcgttt gctgttcagc caggacgaca tcaaaaagcc agctaaagtg
    36301 ctttccggtg gggaaaaagg gcggatgctg tttggtaagt taatgatgca gaagccgaac
    36361 attctgatca tggacgaacc gaccaaccac ctggatatgg aatccatcga gtcactgaac
    36421 atggcgctgg aactgtatca gggcacgctg atctttgttt cacacgaccg tgagttcgta
    36481 agctccctgg cgacccgcat tctggaaatc accccggaac gcgtgatcga ctttagcggt
    36541 aattacgaag attacctgcg tagtaaaggg atcgagtaag tgattatccg tcaccccgat
    36601 actgtcgggg tgacggcggc tgatgacaaa tgcaaaat
//
LOCUS       DAPHDX010000038        36481 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPHDX010000038 DAPHDX010000000
VERSION     DAPHDX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.038"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            173..1390
                     /gene="alaA"
                     /locus_tag="RH058_001793"
     CDS             173..1390
                     /gene="alaA"
                     /locus_tag="RH058_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV3137385.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1474..2073
                     /gene="yfbR"
                     /locus_tag="RH058_001794"
     CDS             1474..2073
                     /gene="yfbR"
                     /locus_tag="RH058_001794"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV3137386.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2132..3964)
                     /locus_tag="RH058_001795"
     CDS             complement(2132..3964)
                     /locus_tag="RH058_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV3137387.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4051..4701)
                     /gene="hxpA"
                     /locus_tag="RH058_001796"
     CDS             complement(4051..4701)
                     /gene="hxpA"
                     /locus_tag="RH058_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV3137388.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4712..5206)
                     /locus_tag="RH058_001797"
     CDS             complement(4712..5206)
                     /locus_tag="RH058_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV3137389.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5289..5744)
                     /locus_tag="RH058_001798"
     CDS             complement(5289..5744)
                     /locus_tag="RH058_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV3137390.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6082..7284
                     /gene="ackA"
                     /locus_tag="RH058_001799"
     CDS             6082..7284
                     /gene="ackA"
                     /locus_tag="RH058_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV3137391.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7359..9503
                     /gene="pta"
                     /locus_tag="RH058_001800"
     CDS             7359..9503
                     /gene="pta"
                     /locus_tag="RH058_001800"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV3137392.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9693..11213
                     /gene="yfcC"
                     /locus_tag="RH058_001801"
     CDS             9693..11213
                     /gene="yfcC"
                     /locus_tag="RH058_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV3137393.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11246..11788)
                     /gene="yfcD"
                     /locus_tag="RH058_001802"
     CDS             complement(11246..11788)
                     /gene="yfcD"
                     /locus_tag="RH058_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV3137394.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11846..12397)
                     /gene="yfcE"
                     /locus_tag="RH058_001803"
     CDS             complement(11846..12397)
                     /gene="yfcE"
                     /locus_tag="RH058_001803"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV3137395.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12453..13097)
                     /gene="yfcF"
                     /locus_tag="RH058_001804"
     CDS             complement(12453..13097)
                     /gene="yfcF"
                     /locus_tag="RH058_001804"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV3137396.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13233..13880
                     /gene="yfcG"
                     /locus_tag="RH058_001805"
     CDS             13233..13880
                     /gene="yfcG"
                     /locus_tag="RH058_001805"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV3137397.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13937..14299
                     /gene="folX"
                     /locus_tag="RH058_001806"
     CDS             13937..14299
                     /gene="folX"
                     /locus_tag="RH058_001806"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV3137398.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14320..15213
                     /locus_tag="RH058_001807"
     CDS             14320..15213
                     /locus_tag="RH058_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV3137399.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15261..16163)
                     /gene="rpnB"
                     /locus_tag="RH058_001808"
     CDS             complement(15261..16163)
                     /gene="rpnB"
                     /locus_tag="RH058_001808"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV3137400.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16360..17133)
                     /gene="hisP"
                     /locus_tag="RH058_001809"
     CDS             complement(16360..17133)
                     /gene="hisP"
                     /locus_tag="RH058_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV3137401.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17141..17857)
                     /locus_tag="RH058_001810"
     CDS             complement(17141..17857)
                     /locus_tag="RH058_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3137402.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17854..18540)
                     /gene="hisQ"
                     /locus_tag="RH058_001811"
     CDS             complement(17854..18540)
                     /gene="hisQ"
                     /locus_tag="RH058_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV3137403.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18630..19412)
                     /gene="hisJ"
                     /locus_tag="RH058_001812"
     CDS             complement(18630..19412)
                     /gene="hisJ"
                     /locus_tag="RH058_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV3137404.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19633..20415)
                     /gene="argT"
                     /locus_tag="RH058_001813"
     CDS             complement(19633..20415)
                     /gene="argT"
                     /locus_tag="RH058_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV3137405.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20681..21250)
                     /gene="ubiX"
                     /locus_tag="RH058_001814"
     CDS             complement(20681..21250)
                     /gene="ubiX"
                     /locus_tag="RH058_001814"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV3137406.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADIETHLVMSQAARQTL
                     SLETGFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21345..22862)
                     /gene="purF"
                     /locus_tag="RH058_001815"
     CDS             complement(21345..22862)
                     /gene="purF"
                     /locus_tag="RH058_001815"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV3137407.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22899..23387)
                     /gene="cvpA"
                     /locus_tag="RH058_001816"
     CDS             complement(22899..23387)
                     /gene="cvpA"
                     /locus_tag="RH058_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV3137408.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23646..24308)
                     /gene="dedD"
                     /locus_tag="RH058_001817"
     CDS             complement(23646..24308)
                     /gene="dedD"
                     /locus_tag="RH058_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV3137409.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24298..25566)
                     /gene="folC"
                     /locus_tag="RH058_001818"
     CDS             complement(24298..25566)
                     /gene="folC"
                     /locus_tag="RH058_001818"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV3137410.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25636..26550)
                     /gene="accD"
                     /locus_tag="RH058_001819"
     CDS             complement(25636..26550)
                     /gene="accD"
                     /locus_tag="RH058_001819"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV3137411.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26706..27365)
                     /locus_tag="RH058_001820"
     CDS             complement(26706..27365)
                     /locus_tag="RH058_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3137412.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27448..28260)
                     /gene="truA"
                     /locus_tag="RH058_001821"
     CDS             complement(27448..28260)
                     /gene="truA"
                     /locus_tag="RH058_001821"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV3137413.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28260..29273)
                     /locus_tag="RH058_001822"
     CDS             complement(28260..29273)
                     /locus_tag="RH058_001822"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV3137414.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29339..30475)
                     /gene="pdxB"
                     /locus_tag="RH058_001823"
     CDS             complement(29339..30475)
                     /gene="pdxB"
                     /locus_tag="RH058_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV3137415.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30574..31569
                     /gene="flk"
                     /locus_tag="RH058_001824"
     CDS             30574..31569
                     /gene="flk"
                     /locus_tag="RH058_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV3137416.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31566..32744)
                     /locus_tag="RH058_001825"
     CDS             complement(31566..32744)
                     /locus_tag="RH058_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137417.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAEERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33037..34257)
                     /gene="fabB"
                     /locus_tag="RH058_001826"
     CDS             complement(33037..34257)
                     /gene="fabB"
                     /locus_tag="RH058_001826"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV3137418.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34416..36422
                     /gene="mnmC"
                     /locus_tag="RH058_001827"
     CDS             34416..36422
                     /gene="mnmC"
                     /locus_tag="RH058_001827"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV3137419.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSHTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         8991 a         9934 c         9090 g         8466 t
ORIGIN      
        1 atatacacct cattattttg tcattattaa gttattaaca gcacaatcga gccttcccct
       61 ctggcaaaat cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta
      121 agctgacttc cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc
      181 cattgaaaaa tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa
      241 agaagcaaaa cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc
      301 agccccgttc ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc
      361 taccgctcaa gggtattgcg attccaaagg tctttactcc gcgcgtaaag ccatcatgca
      421 gcactaccag gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg
      481 tgtatcggag cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt
      541 ggttcctgca ccagattacc cactatggac cgcggcggtt tcgctttcca gcggtaaagc
      601 ggtgcattat ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc
      661 taaaattacg cctcgtacgc gtgggatcgt tataatcaac ccaaataacc caaccggcgc
      721 ggtatattcc aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat
      781 tatcttcgcc gatgaaattt atgacaaaat tctttacgac gacgctgagc atcactcaat
      841 tgcgccgctg gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg
      901 cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg
      961 ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca
     1021 acacgccatt cagaccgcgc tgggtggtta tcagagcatc agtgaattta ttacccctgg
     1081 cggtcgtctt tatgagcagc gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt
     1141 ttcctgcgtg aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt
     1201 taacattcac gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt
     1261 ggtgcaaggg acggcattca actggccgtg gccggatcac ttccgcattg tcacgctacc
     1321 gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg cgtttccttt ctggttatca
     1381 tcagctgtaa tcttaatttc actgccggag attgcatccg gcagcgttat cccgccacaa
     1441 tgacctgatg atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat
     1501 ctctcccgcc tgaaactcat taaccgctgg ccgctcatgc gcaacgtgcg gacggaaaat
     1561 gtgtccgaac acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat
     1621 cggaaatttg gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat
     1681 gcctcagaag tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc
     1741 gctcaggagt acaaggctat tgaaaaaatc gctcagcaaa aactggtcga tatggttccg
     1801 gaagagctgc gggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa
     1861 aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc
     1921 gcggccggaa ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg
     1981 cgtcgcagcc aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg
     2041 ctcgatgaga ttagccagga ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc
     2101 aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca
     2161 ccacacaaac cgccatcacg ataatggtga acggtacccc caacttcaca aagtcactga
     2221 agctgtaatt tcccggacct aaaaccagtg tgttaacagg tgaagaaacc ggtgtcataa
     2281 aggcggcgga tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg
     2341 ttttggcagc agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa
     2401 atagcccaat aaccgccgac aagacaaaca aacaacccag catcatatgt ggcccgtaac
     2461 caccgccaat gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg
     2521 ccacagcaaa cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat
     2581 aggcgctttc agcatctata cagcggaatt tccccatcag caggcaggcg atgatagcgg
     2641 caacaggatt aggaatttca tctgtcagca ttaacgccac cattagcacc agacagaaaa
     2701 tggcatgggg tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca
     2761 gcgctacgaa gtcgcggccc tgtttggcca gcataccgat cagtttccag ttacccacaa
     2821 ccaggatgat atcgcccagc agcagaggct catccgccag cgaaccttcc agcgccacgc
     2881 cattgcgctt tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca
     2941 ccgatttacc aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa
     3001 gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac
     3061 aaaattgccg gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc
     3121 gaaattcaga aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa
     3181 caccgatgac gttagcgcca taacgctcac gtaatttgag atcatccagc cgttgaccta
     3241 tcattggcga tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac
     3301 ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc
     3361 ctttcagcat gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc
     3421 caataggtgt tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt
     3481 tgactaccag gttcggcggc gtcgccacca gcgtcatcat gccgctaatc agcccggcaa
     3541 aacttaacgg catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca
     3601 ccacgggaat aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga
     3661 cggtcagcat caacaaaacc aacattttga tttcactgtt gcccgccact ttgaccagcc
     3721 atgttcccat tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg
     3781 cggcaatcag gacaacgtta ggatcagaaa agccggaaaa tacttcgggg accgtcagcg
     3841 ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc
     3901 ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt
     3961 tcacaactta tccttgttaa ttgaggggga tgacttgatt ctgccataaa aaagccccga
     4021 cgagacgggg ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg
     4081 tttggtcaca gtaatttgct ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg
     4141 ggtatctgcc ggagcgttaa ccgcaatgac atggcaaccc gccgccaggc cagaaagcac
     4201 gccagcggga gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc
     4261 acctaacaga tacgcatcag gttctggttt tccgcgcttc actcgctcag cggttacaaa
     4321 cacctctggt gcgggaagcc cagctatttt atggcgcgct cgcgctaccg gcatggagcc
     4381 agaagtcaca atggcccacg gaatacctgc tttattcaaa tgattgagta aggcgattgc
     4441 ccccggaagc gcggtaatac cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa
     4501 ctcggcggca atatcagcct cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc
     4561 ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct aacccatgac gtctggccca
     4621 gttgctccac gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa
     4681 cagaaaacct ttgcaccgca cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt
     4741 tggcgctcaa atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt
     4801 tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc
     4861 gcccttccac attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt
     4921 caaagccgag gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc
     4981 aggaaacatg caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt
     5041 ctttcagctc gccaaactcg cgatccaatt cacgcatctg taatccgtaa ccacgctcaa
     5101 taattgtttg caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct
     5161 tgtactgatt agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta
     5221 tcactatact acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca
     5281 ggggtcaatt acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc
     5341 gtgtcagcta atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac
     5401 tcctgcaatt taccgcgaac ttcataaaac cagttaagga ttgcaggggg taatggcgtt
     5461 acagaacgct tgcccagcca ccacaatccc tgcatgggta aacttaaggc gaacagcgca
     5521 gtggcaactg ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg
     5581 gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca
     5641 aagaccgggg caaggcgttt ttccagcggc cacgtctttg agtaatgctg tccccggcga
     5701 aacaagctaa aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc
     5761 acatataaag attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca
     5821 ttttgtcttc catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca
     5881 tattcattga gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg
     5941 gcatagactc aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat
     6001 cataaatgtc ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac
     6061 gtatcaatta taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt
     6121 tcttcactga aatttgccat catcgatgca gtaaatggtg aagagtacct ttctggttta
     6181 gccgaatgtt tccacctgcc cgaagcacgt atcaaatgga aaatggacgg caataaacag
     6241 gaagcggctt taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact
     6301 attctggcac aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta
     6361 cacggcggcg aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc
     6421 aaagatgcag cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa
     6481 gctctgaaat ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc
     6541 caccagacta tgccggaaga gtcttacctc tacgccctgc cttacaacct gtacaaagag
     6601 cacggcatcc gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg
     6661 gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac
     6721 ggtggttccg tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc
     6781 ccgctggaag gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc
     6841 cacctgcacg acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagag
     6901 tctggcctgc tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac
     6961 gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac
     7021 atcggtgcct acactgcgct gatggatggt cgtctggacg ctgttgtatt cactggtggt
     7081 atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc
     7141 tttgaagttg atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac
     7201 aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa
     7261 gacgcgagcc gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg
     7321 taacccgcca aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga
     7381 tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg
     7441 aacgcaaagg cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg
     7501 atgcgcccga tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg
     7561 aaccgctgaa aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga
     7621 tggaagagat cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg
     7681 aaggtctggt cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta
     7741 aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc
     7801 tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca
     7861 ccggcgttat cgttaacaaa ctgaacgcac cggttgatga acagggtcgt actcgcccgg
     7921 atctgtccga gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga
     7981 agctgcaaga atccagcccg ctgccggttc tcggcgctgt gccgtggagc tttgacctga
     8041 tcgcaactcg tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg
     8101 acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc
     8161 tggagcactt ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg
     8221 tggccgcttg cctggcagcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg
     8281 gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc
     8341 cggtatttat ggtgaacacc aacacctggc agacctctct gagcctgcag agcttcaacc
     8401 tggaagttcc ggttgacgat cacgaacgta tcgagaaagt tcaggaatac gttgctaact
     8461 acatcaacgc tgactggatc gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt
     8521 ctccgcctgc gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg
     8581 tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg
     8641 gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc
     8701 agggtgtaga actgggtgca gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct
     8761 atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg
     8821 aacagctgga agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg
     8881 gtctggtttc cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga
     8941 tcaaaactgc accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac
     9001 aggtttacgt ttacggtgac tgtgcgatca acccggatcc gaccgctgaa cagctggcag
     9061 aaatcgcgat tcagtccgct gattccgctg cggccttcgg tatcgaaccg cgcgttgcta
     9121 tgctctccta ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag
     9181 caactcgtct ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg
     9241 acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc
     9301 gcgctaccgt gttcatcttc ccggatctga acaccggtaa caccacctac aaagcggtac
     9361 agcgttctgc cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta
     9421 acgacctgtc ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga
     9481 ttcagtctgc acagcagcag taatctcgtc atcatccgca gctttgcgct gcggatatct
     9541 gaaccggaaa taatcactat ttccggtttt ttattctctt aatctgcatt aatcctttct
     9601 gattatcttg cttaactgcg ctgcatcaat gaattgcgcc atttcacttt gcatacttac
     9661 cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa
     9721 caagaagatg ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca
     9781 gccttgccac ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg
     9841 ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta
     9901 acgaagcagg cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc
     9961 cgggcctgat gaacttcccg tttgaaggat taacctcagg atcgaaatac gggacagctg
    10021 ttggcatcat catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag
    10081 gaaccattga taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct
    10141 ttattcctgc gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag
    10201 aggccgtcgc ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta
    10261 ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga
    10321 acccgttttg tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg
    10381 ggttgcgcat cgtggtgtgg gttatcgcca ctctgattgg cctgatcttt accatggtgt
    10441 acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct
    10501 ttcgtgaaaa gcaggctgat gttgaacaac gtccgtttac ctttggtgac tggctggtat
    10561 tgattgtcct gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt
    10621 ttattccaga aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg
    10681 ttgttttccg ccttaacggc atgacggtta ataccatggc ttcatccttt accgaagggg
    10741 cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg
    10801 tcggtaatgg tgaagcaggt gatgccagcg tgttaaatac catcctcaac agcattgcca
    10861 atgccattag cggtctggac aacgctgtcg cggcctggtt tatgttgctc ttccaggcgg
    10921 tatttaattt ctttgtgaca tccggttctg gtcaggcggc gttaaccatg ccgttactgg
    10981 caccgcttgg cgatctggtc ggtgttaacc gtcaggttac cgtgctggca ttccagtttg
    11041 gtgatggctt cagccacatc atttacccaa cctcagcttc gttaatggcg acgctcggtg
    11101 tttgccgggt ggacttccgt aactggctga aggtgggcgc gacactgctt ggactgctgt
    11161 ttattatgtc cagcgtcgtg gtgatcggcg ctcagttgat gggctaccac taaaaatgtt
    11221 aagagccgca attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt
    11281 tggcattgcg cttcatccac aacgccagcg ctttcagcga gtctggagtg aactcatcgc
    11341 agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta
    11401 gggcgaaggg accgtgagag acgcagctga acaatgcgcc ccagacacgg caatttttat
    11461 cttcgaaata gaactgcccg tgctcagcaa aggggacacc ggcaatgccc aactcttctt
    11521 ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc ctggaccaca ccgcctgcgg
    11581 tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt
    11641 tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca
    11701 tttgttcccg gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt
    11761 cagtacttgc caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta
    11821 cggcgcattt tgagttgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt
    11881 gatcattgag tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat
    11941 taccgccttt cggaatactc accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt
    12001 ctgccactgg cagatgggta tggccgtaca ccagcacatc gttctggttt aaagcaggca
    12061 gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata
    12121 cctgttgcca cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc
    12181 agttgccgcg cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg
    12241 ctggcgcgta accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga
    12301 tcaccagcca ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta
    12361 acgacccatg aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac
    12421 gtaagctatg atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca
    12481 ataaaacgct ggacagacgc tcgctgccac tggaatgtcg cataatccac caggcgttct
    12541 ggcacctcat cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca
    12601 atgcaccatt caccaaataa attcggctga cccagtacta acaaatgttc tgccatcgca
    12661 aacagtttct ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca
    12721 aagacaacat ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc
    12781 tgaatctgtc gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc
    12841 ggtggcgcaa atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa
    12901 tcatcgattt gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc
    12961 aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc
    13021 aacgccaccc aggcggataa cacataaggg gagaaaaagt gggcatctga ccaaagcgtg
    13081 atagcgggtt tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag
    13141 cctttttgtc actgtcacgt gacgaatact cacgtaagtc gccgcgcttt ctatacttgt
    13201 ggtgttcaga tgattaaaca accggagctg caatgatcga tctctatttc gccccgacac
    13261 ccaatggtca caaaattacg ctgtttctcg aagaagcaga gcttgattat cgcttgatta
    13321 aggtagacct ggggaaaggc ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca
    13381 acaaaattcc ggcaattgtt gatcattctc ctgccgatgg cggcgaaccg ttaagcctct
    13441 ttgagtctgg tgccattttg ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg
    13501 aaacgcgtga gcgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc
    13561 cgatgcttgg gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta
    13621 ttgaacgtta tcaggttgaa actcagcgtc tttaccatgt actgaacaag cggctggaaa
    13681 actcgccctg gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg
    13741 ttaatgcctg gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc
    13801 atgagcggat ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg
    13861 atgagcgttc ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa
    13921 caacggagaa cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt
    13981 gcgtacgttt atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa
    14041 tgtgacgatc cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct
    14101 gaattatcgc accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt
    14161 gctggaaaaa ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta
    14221 tgctgaagtg gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac
    14281 cttaagctgg cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga
    14341 gggacgggat taattggtcg ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc
    14401 acggtagtga cgcgtaaccc acagaaagcc agttccgttc tcggccctcg ggtgacgcta
    14461 tggcaagggc tagccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc
    14521 ggagaaccga ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc
    14581 cgctggaata tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccgccgtcg
    14641 gtactcattt ccggctcggc aacgggctat tatggcgact taggtgaagt ggtggttacc
    14701 gaagaggaac cgccgcataa cgaatttact cataaactct gcgcccgctg ggaagagatt
    14761 gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc tgcgtaccgg cgtagtgctg
    14821 gcaccggatg gcggtattct cggtaaaatg ctgccgccgt ttcgtcttgg cctgggcggg
    14881 ccgattggtt ccggtcggca gtatctggcc tggattcata tcgatgatat ggtcaacggc
    14941 attctctggc tgctggataa cgagctgcgc gggccattta atatggtttc gccctacccg
    15001 atacgcaatg aacaatttgc ccatgcgctc ggtcatgcgc tgcatcgccc ggctattttg
    15061 cgcgtccccg caaccgccat tcggctgtta atgggcgaat cttcagtact ggtattaggt
    15121 ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ttggtacgat
    15181 ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt tactgcaaac atccgccagt
    15241 taactcccgg tgttacaggg ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg
    15301 gtcaccatca taactaactc tcggtctaaa cctctatcca gcatctcctg agcaatacgc
    15361 agggcttctt cgcgtttgcc aagaattaat ccatcattcc gcccttcttc acgtaatctg
    15421 tcagcaatgg tcatcagttt ctccttttcc tgtggtgcgc gttctgctat ctcaccaata
    15481 aatgcacgga aacgctgggc atctcccgtt tgtaatacat aattaaacag ggcttttagc
    15541 tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac
    15601 agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt gcgatgttgc
    15661 ataatctcgt catccggcac cacggtaata tccaccaacg gaaaagccga tgaatatatt
    15721 ttacgggcaa tcgcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga
    15781 cttctgcaac catgataaaa cagcattggg atcaccaatg gaagctcttt atagcctgca
    15841 tccagatggt tttgcatcgc cgcgatggaa tagcgcatca tgcgaaaagc cattaattct
    15901 tccggcttac tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc
    15961 acagaccaca agaggtcgga ataatattgc cgcaggtcgt catcaataaa actgtttggt
    16021 tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca
    16081 ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc
    16141 gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac
    16201 aataaccacg attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag
    16261 aagtattttt agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc
    16321 atgaaaatac tccggcagcg ttagccaccg gagaggaaat tatttcagcg atcccttaag
    16381 gaactgttgc agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccctcttc
    16441 ttctattttc ccctgatgga ggaaaatgac gtgagtagaa acatggcgag caaagcccat
    16501 ttcgtgagtc actaccacca tggttttccc ctcttctgcc agttgctgca taatacgtaa
    16561 cacttcgcct accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc
    16621 cggttccatc gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg
    16681 caccggatat ttcccctgcg cacgttcgtc tatcccgact tttgccagat acttcaccgc
    16741 ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt
    16801 ttccagcacc gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt
    16861 gcgcagtaag cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac
    16921 cagattgatc gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt
    16981 aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc
    17041 tccggcattc gcttgcagtg agaccccttt cagcacttca tgttcgccgt agcgtttgtg
    17101 caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt
    17161 tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga
    17221 tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt
    17281 taatatcgcg ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag
    17341 agtgcagcat caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag
    17401 gcaaaataat gcaacgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa
    17461 tttccccatg cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg
    17521 tgttaagcgt cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga
    17581 attcggttcc cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa
    17641 catacagcgg cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt
    17701 tattactgga gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg
    17761 acaaaataag cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca
    17821 gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac
    17881 ggagtagcgg cgctcaagga acagcagcac accattggaa acagtggtga aaaccaggta
    17941 aatcacgcca cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagttg
    18001 ggtggctttg accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat
    18061 cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat
    18121 ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt
    18181 cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc
    18241 gagagtaata ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc
    18301 cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca acaccaagtc
    18361 cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg
    18421 cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta cagaactgat
    18481 agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat
    18541 gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa
    18601 gggagcgggc tttctttcac cgatggcgat tagccaccat aaacatcaaa atcgaagtac
    18661 tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt
    18721 gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt
    18781 ttttcatctt taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg
    18841 aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg
    18901 tcagaataaa tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccaa
    18961 tgttcgttac cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg cttgcctttc
    19021 agcgactcga ctgtcggctg aatgtcggaa tttttcgcca ccaccagacg agaatcggca
    19081 gcgtacagtt tgtcggtgaa ggcaatttct tgctgacgtt tttccgtaat ggaaagcgat
    19141 gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt
    19201 tcgacaaacg tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg
    19261 aagccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta
    19321 ccgatgcgga tgttttgcgg aatcgcagca aacgccgcag ttgcgctgga gaaggccaga
    19381 accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata
    19441 gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat
    19501 atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc
    19561 accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt
    19621 cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc
    19681 gtaggtgccg tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc
    19741 atcatcttta cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga
    19801 tgagccagca aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc
    19861 aacttcatct tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc
    19921 ctggttggca taggccacca catccacgcc tttgctacgc caggtctcgt tagcgtaagc
    19981 ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg
    20041 ctgaatcggt gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga
    20101 gaaggcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat
    20161 ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt
    20221 cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc
    20281 tttagcatct ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc
    20341 cggtagcgcc gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag
    20401 aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg
    20461 ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat
    20521 tcaacgttaa atatgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt
    20581 atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat
    20641 cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg
    20701 caaagagatc ttcaggaagg gttatcgcaa actggtcaag aacacgatta accgtctgat
    20761 ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca
    20821 ccgcaccgat ttctgctgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt
    20881 cacgcacgca gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca
    20941 gtaaaccatc ggtgtagcta tggacaatgc cggaaagggt tttgattgaa cagggtaaaa
    21001 taaccatccc cagcgtctgg aaagaaccgg aagagatgct ggcggcaata tcgcgcgcat
    21061 cgtgcgtgac atcagctaat gcctgcactt cgcgcaggga aaaacccgtt tcgagggata
    21121 aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc gatatctgcg acatcgcgca
    21181 gaacctgtaa taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga
    21241 gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagg atttcggcgg
    21301 gagttgcaag tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc
    21361 atttcgagat tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac
    21421 gtatcgagga aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac
    21481 accgagcatt caaactgctg gatatccgga ttttcagcgc gaacggcgtc gatcagatcg
    21541 ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg
    21601 cgaccgtgag cgataagctc cgtggcgctc ggcatatcaa taccataaac gttcgggaag
    21661 cgaatttccg gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc
    21721 tcgataatct gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt
    21781 ttatcgcgga actcggcgcg gttggcattc agtttacggc gcacggactt acgacgcagc
    21841 tgctggcccg gcatgataaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg
    21901 tacggtttgc ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttctgggatc
    21961 gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc
    22021 gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga
    22081 gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg
    22141 gtaaacaact gcccttcttc agtaatgtaa atcgcttcgc caggcgcgac gtcacgcagg
    22201 aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag cgaccatata ttctgtacgg
    22261 ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa
    22321 gcaaccatac cgtggccgat aatcatcgcc acacaggcat atgcgccgcg gattaagcgg
    22381 tttgtggcag caatggcagc gaaaatattg tcggcttcca gcgggtagtg gcggaaattg
    22441 tccagctcgc tggcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg
    22501 cggcgttttt cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg
    22561 tgggcaagcg taatgccata cggggagtta acgtaaaacg gctgcgcttc agaggcgctg
    22621 gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc
    22681 atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag
    22741 ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt
    22801 aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac
    22861 atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt
    22921 gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaactg cgggatcagc
    22981 tgtgatttgc tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga
    23041 atggcggcaa caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg
    23101 gtgcctgaca accccgtttt ctccaccagc tggcctatca cgaagttcac gatagcacca
    23161 acgatcaggg tagcgataaa cagtaccgcg atggcaattc catttcgaac cagttcgtct
    23221 tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca
    23281 caaccccatg tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga
    23341 gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga
    23401 ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta
    23461 gggatttcct tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac
    23521 tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg
    23581 acctacggtt ctaacactgc ccaggcctga taagacgcgc cagcgtcgca tcaggcaaga
    23641 ctgtattaat tcggcgtata gcccattacc acgccactta agccagaaag ttgcttcaac
    23701 tcacccagcg aacctttcag cttatctttc gaggcatccg gcccaaccag aatacgggta
    23761 attttaccct gcactggcgt ggatggcgac gtataaaccc gataaccggc accgcgcagc
    23821 ttaccgacaa tctcattcac tttatcggca tttttcagcg cacccagttg cacaacatag
    23881 gctttacccg ttggcgctgc tttttcttcc acgaccggct tcggttctgg cttcggcgca
    23941 ggtggtgctt caaccttcgg tttaggcggc tccaccggtt tcggcttcgg tggggcgacc
    24001 ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa tagtggtcgg atcgagcgac
    24061 ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc
    24121 aacgcctggg tggcggctgg catcatatca ggctcatcgc gatcgcccgc tttcggcacc
    24181 agcgggatag ccgcgaactc atcctgataa tgttttttct gcccgtccag cagccctgga
    24241 agtacaatca cccccagcgc caccagcacg atcgtgccca ctaaccgatt ctgaaactta
    24301 cttgccaccg cttctcctcg cgtcaatcac ttccatgaca tgtgcgacag tgtggaaaga
    24361 accacacacc agcacggtgt cttccgcttt agcgtccgcc attgcggcat cccatgcctg
    24421 cgcaacgcta tcaaatgatt tgccgttacc caaatgctca agcagttgtt ctgccgtggc
    24481 accgcgcggc ccttccagtg gcgcacaata ccagtcatca accacgcttt tcaaccaggc
    24541 cagagttccg gcaatatctt tatcatgtag cataccgata accgccagca cgcgcccgtt
    24601 tttcggtagc gctttcatac gcccggtgag atattccgcc gcatgtggat tatgcgcgac
    24661 atcaaaaata acgcgtggcg actcgctcac aatctggaaa cgtcccggca aaattgcgct
    24721 ggcaatcccg tcgcgaatgg cattttcact gacttccagc ccgctggcac gcagtgccgc
    24781 cagcgccgtt gcggcattcg gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc
    24841 gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa tagttccact caacgccccg
    24901 acgttgtaac agtgcacctt tttcctgtgc cacatcagca atggtagaag gcatttccgg
    24961 ctcaccgaca attgccggtt tttcgctgcg gaagatgcct gctttctcgc gaccaatact
    25021 ttcgcgatct ggacccagcc agtcggtatg atccagcgca atactggtta ctaccgcgac
    25081 atcggcatcg acaatattgg ttgcgtccag acgaccgccc agccctactt ccagaatcac
    25141 cacgtcaagt tgtgcctgct tgaacagcca caacgccgac agcgtaccgt actcgaaata
    25201 ggtcagggaa atatcaccgc gtgccgattc aatctccgca aaagaggcgg tgtgggccga
    25261 ttccggcaat tcctggccct gcacacgtac gcgctcggta taacgcacca gatgcggtga
    25321 actgtagacg cccactttgt accctgccgc catcagaatc gactccagcg tacggcaggt
    25381 ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc
    25441 aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga tcgatagttt tactgtgcag
    25501 gttttccaga taagaaagcc acgaagccag aggcgacgcg gcttgaggag tgcgtttgat
    25561 aatcatggta tccgctgatt cgttacggtg agaatagcaa aagggcagag ccagtggccc
    25621 tgcccttatc agttatcagg cctcaggttc ctgatccggt accgggggta ccactacgcc
    25681 ttcacgcggc gcttcaggat tcggcgctgg cagattcatc aacttcgcca gaatgctcgc
    25741 cagtttcagg cgcatttccg gacgacggac gatcatgtcg atggcgcctt tctcgatcag
    25801 gaattcactg cgctggaatc ctggcggcag tttttcgcga acggtctgtt cgataacacg
    25861 cggaccggca aagccaatta acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc
    25921 gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc
    25981 gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc atctgcatca gcgacatcag
    26041 tgcttcctgc atacgtgcgc caccagaggc ggagaagcag atcagcgggc agttatcttc
    26101 cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca acagacccca ttgaaccgcc
    26161 cataaaggcg aactcgaatg ccgcagcgac aaccggcatt ccatacagag tgcctttcat
    26221 caccaccagc gcatcttttt cgccagtttc tttctgcgca gatgccagac ggtctttata
    26281 cttcttggag tcacgaaact tcagcacatc tttcggctca agctcgctac ccagctccac
    26341 aaggcttcct tcatctaaca ggctatgcag gcgattacgc gctgtcatac gcatgtgatg
    26401 gtcacacttc ggacagacct caagattacg ttccagctca gcgcggtata aaacctgacc
    26461 gcagctatca cacttagtcc acaccccttc aggaatgctc gccttgcggg tgggagtaat
    26521 gttgctttta attcgttcaa tccagctcat tagggacctt tctgtctgaa cctggttcga
    26581 tgccagtttt atctttgggg acgcataatg ccatttttgc ccccaacaga ccatgaatgt
    26641 tgcacattaa aacataacag cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt
    26701 actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac ggattatttc gatgacgccc
    26761 ggcaaaatag aaaccacaat aatcccgacg atcagcagct taaggttatc ctgaaccatc
    26821 gggattgtac cgaagaaata gcctgcgtag gtaaaaagca gtacccacaa cagtgcgccg
    26881 atcacgttat aagcggcgaa atgacggtac gacatgtggc ccattcccgc aacaaacggg
    26941 gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa tcgttttgcc gccatgtttc
    27001 tcataaaact gatgggtttt gtcgagataa ctgcgacgga aaattttcga attgggatta
    27061 ctgaacaact tttcaccgaa caaccgcccg atagtgtaat tgaccgcgtc acccacaatc
    27121 gcggcgatca gcatcagcac caccatcata tggacgttga gatcgttggt ttccagtgat
    27181 gccaacgctc cggcaacaaa cagcaatgaa tcacccggta aaaacggcgt taccaccaga
    27241 ccggtttcac agaacaaaat taagaacaaa atggcataaa cccagacgcc gtactccgcg
    27301 accagctctg ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg
    27361 tccatatttg cctatgcctt gtactcgtca tctttcaggc tgtaactacg ttggctacag
    27421 cctgaaatgt tccgagcatt attctcgtta gtccgccaga aatagcgggc ccattggcgg
    27481 ttttggaaga tcataccggt cagggtaatc caccgcgacc agatacagcc cttccgcttt
    27541 tgccgttgct gccgccagcg ttctgtcctt tgccgccagc aactctgcta tccagctctc
    27601 cggctggttg tgggcaccga cttccatcag gctgccgaca atattcctga ccatatgatg
    27661 tacaaaagca ttcgctttaa tatctaccac cacataagga ccgtgacgcg tgacattaat
    27721 atgcataacg ttgcgccacg gggttcggga ctggcactgc accgcacgga acgaggtgaa
    27781 atcattctcg cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc
    27841 gtaaaaatgg gttacccctt tactcagtac cgccgggcgc agccgatgat tgtagatgat
    27901 gtagcgataa cggcgagccg tggcgctaaa tcgggcatga aaatcatcag gtacagcttt
    27961 aacccaacgc acggcgatgt caccaggtaa attcgcattt acgcccaagg tccacgccgc
    28021 gtctttgcgc tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt gtacccctgc
    28081 gtcagtacgc ccggcgcaga agacggtgat gggttcgttc gccacctggg agagcgcctt
    28141 ttccagcttc tcctgcacac tgcggacttc attctgccgt tgccagccgt aatacttact
    28201 gccgtcgtac tcaatgccca gcgcaatttt ataaactggc agttgttgct ggtcggacat
    28261 tagtacagat actcctgcac cagtttctcg gcgattttta ctgccatcag cgcgccgcca
    28321 aagcgaacgt tatcggccac cgaccagaac tggacttgct ccggcatacc gtagtcatta
    28381 cgcacgcagc caacagaaag atgcggcgta cccgaagcat cccctacctg agtcgggaat
    28441 tcgttctctt cagagagcac aatatcttcg ccctgagcaa acgcatcacg cgcttcttct
    28501 gccgccagcg gacgcagtgc ttcaaagttg accatctggg catgaccgta gaataccggt
    28561 gcctggacga cgcttgccga aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg
    28621 tcaacgatac gacgttcttc acgcacgcta ccttcgctat ccggcaacaa cggcagcata
    28681 ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat
    28741 ttcgcactct gccccgctaa cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc
    28801 aggctggtaa cactgatacg cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc
    28861 agcagctggc tggtcagact gtctggtacg gcgatgacat tccggttgcg gtaatcggtc
    28921 agtacaaacg ggtttacttc cggcaccacc agcggtacgt cgggttcgag agcaaacagt
    28981 ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt cttcaaccca ggcagcggta
    29041 gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca
    29101 tcctgcacgg tgattgtttt accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt
    29161 gccagtgcat aaatttcccc aaccgggaac tgacgttcag ccagcgtttc aagcagggct
    29221 tcgcccacag cgccagttgc gcccaggacg gcaatgttcc agccttcaga catggtggtt
    29281 tactccagaa atagcaaagc tccctgccaa tgttacagca gagagcatga agaagagatt
    29341 aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca cagcaatgat gccgcactgg
    29401 cgtcatcaca aattacatac agagatgacc attcacggcg ctcaagatag tttttgcgca
    29461 gtttatcgaa ctcacccggt atcccggcga ctttacgcag cggtgcgtca tcgcggcgca
    29521 catcatacac caaatgcacc agccttttca gcgtcggttg atcgagcggg ccatgcagcg
    29581 taatgcgacc aaactctggc gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc
    29641 caataaactt gctataggct tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg
    29701 tataacctgc gatatgcggc gtgccgatat ccacttttgt cagcaactcc acgttaagtt
    29761 ccggttcgcc ttcccagaca tccagcacta cgcttaactt ctggccttca ttcaggcagg
    29821 tcagcaacgc agtattatcg acgactgcgc cacggcaggc gttaatcaga atcgctcccg
    29881 gcttcaggct acggatcagt ttttcatccg ccagatgcag cgttttgtac ggcccatctt
    29941 tgaagagtgg cgtatggaaa gtcagaatat ccgcatgctg aactaattca tccagcgagc
    30001 ggaaatcacc ctcatccccg cggtcagcgc gaggcggatc gcaaagcaat gttttaatcc
    30061 ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc acgatcccca
    30121 ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag catcagtagt gaggagaaaa
    30181 catattccac caccgcaatc gcattacagc caggtgcagc ggaaaaacca attcccgcct
    30241 gcttcaacca tgcttcatcg acatggtcgg tccccgcagt ggcagtgcca acaaatttaa
    30301 tgggttttcc tgccagcaaa gattcattca ctttcgtgac cgaacgcacc atcagcgcat
    30361 ccgcgtctgc cagttgagca acggggattg gacgcccggg aaccgcggtc acctcaccca
    30421 aacggctaaa taagtcgcgg gcataaggca tattttcatc aacaaggatt ttcacgtttg
    30481 tgttacctgt atgagacgag agttaaccgg acaagtgtgc cataatctcg cggccaggca
    30541 tacttgcgaa gatttcaggt ataaggatac gtaatgatac aacctatttc cggccctcct
    30601 cctgggcaac caccaggtca gggagataac ctgccgtctg gcgcgggcaa tcagccttta
    30661 tccagtcagc aacgtacttc gctggaaagc ttaatgacga aagtgacctc actgacgcaa
    30721 caacaaagag cagaactgtg ggcgggtatc aggcacgata ttggtctgtc gggagattca
    30781 ccgctgcttt cgcgtcactt ccctgccgct gagcataatc tggcgcaacg tctgctggcc
    30841 gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat taggggagta tttacgtctg
    30901 gggaataatc gtcaggcggt cacggattat atccgtcata actttggtca gacgccgctg
    30961 aatcagctct caccggagca attaaaaacc attctcaccc tgttgcagga agggaagatg
    31021 gttattccgc aaccacagca gcgcgaggcg accgaccgtc ctttattacc ggcggagcac
    31081 aatgcgctaa aacagctggt gaccaaactt gcggcggcaa cgggggaacc cagcaaacag
    31141 atctggcaat cgatgctgga actttccggg gtgaaagatg gcgagttaat tccagcgaaa
    31201 ctgtttaacc atctggtgac ctggttgcag gcgcgccaga cgttaagcca gcaaaatacg
    31261 ccgacgctgg aatcactaca gatggcgcta aaacaacctt tagatgccag tgaactggcg
    31321 gcgttatcgg catatatcca gcaaaaatat ggcctttctg cgcaatcatc gctttcttct
    31381 gcccaggccg aggatattct taatcagctt tatcaacggc gggttaaagg gattgatccg
    31441 cgtgatatgc aaccgctgct taatcctttt ccaccgatga tggacacgtt gcaaaatatg
    31501 gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct ggtctggctg
    31561 gttcgttagc cccgacgaaa tgacagtatc gtgacaataa ttcccagcac cgcagagatc
    31621 gccccggcaa gaaataccga agagtaacca aacgtggtcg ccagcattcc cgccagcggc
    31681 ccggagacgc cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga
    31741 acttgtgagg ggacgcgttt aaccacctcc acgcccagcg caggaaagat aagcgaacat
    31801 ccggctccgg ttaacgccgc gcccgctaat gcgacccacg cacctggggc ttgccagagc
    31861 agcaacaagc ccaccgtttc tacaagcaga gagacaatcg ccactttcac gccgccaaaa
    31921 cggtccggca tccagccaaa catgacgcgc atcacgacaa atgcgccgcc aaacgcggta
    31981 agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg
    32041 ataaccgcaa aaccaacgcc ttgtagtgcc agacctaacc ctggtttcca gataagcccg
    32101 acaacgctcc acagcgatgg acgttcttcc gccagggccg gtactttgcg cactgtgccg
    32161 ttacaggccc acgccagtac gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg
    32221 taatggctat gaatcaacag gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc
    32281 attccgttcc atgacatcac tttgccagag tgttttggcc ctacgatgcc tagtccccag
    32341 gtcagagcgc ctgtcagtaa ctggctttca ccaaagccaa gaatcaaacg cccgacgacc
    32401 aacagagcaa atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg
    32461 ccagccagac cacaagctaa cattccctga agcgccgaac gttttgcacc atattgatcg
    32521 gccagtcgcc cggcgtaacc acgcgtcagc accgtagcca gaaactgaat cccgacggca
    32581 atgccgacca tggtattgcc atagcccagt tcatgatgaa caaacagcgg gataaccggc
    32641 aacggcaacc ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa
    32701 aaattggcag aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg
    32761 cagcgcaagg cgaggagtat ccccgtctca tctctctggt ttcagggtta cggtgcgttg
    32821 gcaggattta acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg
    32881 aactggcggc gattgtcaat gatgtggaaa agggaaccat caggttccct tttgcgttag
    32941 tgccggaggc cgcattggcg cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct
    33001 actccgacta ctccgactac tccgacctac tgcgaattaa tctttcagct tgcgcattac
    33061 cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac ataacggtgg tcagttcgcg
    33121 atcggtcgtt tcggtcacga tgttcagacc tgcagcctgc tcgtccagct cttcaatgtt
    33181 gatgctcggg gcgataaagc cgtgttccag catcagcaga gagtagatag cttcctgtac
    33241 gccagcagcg cccagagagt gaccggtcat ggctttggtt gcagaaatcg ccgggctctt
    33301 atcgccgaac acttcacgga tagctgccag ttctttcacg tcgccaaccg gagtcgaagt
    33361 accatgagag ttcaggtaat cgattggggt atcaacgccg tgcatcgcca tcttcatgca
    33421 gcgtactgcg ccttcgccag acggagcaac catgtctgca ccatcagagg ttgcgccgta
    33481 gccaacgatt tcagcataga tgtgagcacc acgcgccagc gcgtgttcca gctcttcaac
    33541 cactaccata ccgccgccgc cagcgataac gaaaccgtca cggtgagcgt cgtaagtacg
    33601 ggaggctttt tccggggtgt cgttgtattt agtagacagc gcacccatcg cgtcgaattc
    33661 gcaggccatt tcccagcaca gctcttcgcc gccgccagca aacacgatgt cctgtttgcc
    33721 cagttggatc tgctctactg cgttaccgat acagtgtgcg gaagtcgcac acgcggagct
    33781 gatggagtag ttaacgccat gaattttaaa cggcgtggcg aggcaggcag aaacgccgga
    33841 tgccatcgct ttggtgacca catacgggcc aaccgctttc aggccgcgcg ggccgcgcat
    33901 tgcgtcagcg ccgaacacct ggaaacgcgg ggaaccgccg ccagaacctg caatcaggcc
    33961 aacgcgcggg ttattctggt aagcttccgg agaaaggccc gcatctgcga ttgcctgctc
    34021 catagaaagg aatgcataaa tggatgcgtc gctcataaag cgcacaactt tgcggtcaat
    34081 gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg ctacgcatgc cggaatcctt
    34141 cagctcctga gagaaagtga tccctgaacg tccttcacgc agagatgcca ggacttcctg
    34201 ctggttatta ccgatgctgg aaacaatgcc caggccagta atcactgcac gtttcattca
    34261 atacctctgt aagtcgcaca tagagtaagt ttcgaatgca caatagcgta cacttgtacg
    34321 ccgaacaagt ccgatcagcc atttaataga gaaatttgcg cagccttaca cacatcgcta
    34381 agatcgagcc accgcctgta agacgagtaa cttacgtgaa acactactcc atacaacctg
    34441 ccaacctcga atttaatgct gagggtacac ctgtttcccg agattttgac gatgtctatt
    34501 tttccaacga taacgggctg gaagagacgc gttatgtttt tctgggaggc aaccaattag
    34561 aggtacgctt tcctgagcat ccacatcctc tgtttgtggt agcagagagc ggcttcggca
    34621 ccggattaaa cttcctgacg ctatggcagg catttgatca gtttcgcgaa gcgcatccgc
    34681 aagcgcaatt acaacgctta catttcatta gttttgagaa atttcccctc acccgtgcgg
    34741 atttagcctt agcgcatcaa cactggccgg aactggctcc gtgggcagaa caacttcagg
    34801 cgcagtggcc aatgcccttg cccggttgcc atcgtttatt gctcgatgaa ggccgcgtga
    34861 cgctggattt atggtttggc gatattaacg aactgaccag ccaactggac gattcgctaa
    34921 atcaaaaagt agatgcctgg tttctggacg gctttgcgcc agcgaaaaac ccggatatgt
    34981 ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg tccgggcggc acgctggcga
    35041 catttacgtc tgccggtttt gtccgccgcg gtttgcagga cgccggattc acgatgcaaa
    35101 aacgtaaggg ctttgggcgc aaacgggaaa tgctttgcgg ggtgatggaa cagacattac
    35161 cgctcccctg ctccgcgccg tggtttaacc gcacgggcag cagcaaacgg gaagcggcga
    35221 ttatcggcgg tggtattgcc agcgcgttgt tgtcgctggc gctattacgg cgcggctggc
    35281 aggtaacgct ttattgcgcg gatgaggccc ccgcactggg tgcttccggc aatcgccagg
    35341 gggcgctgta tccgttatta agcaaacacg atgaggcgct aaaccgcttt ttctctaatg
    35401 cgtttacttt tgctcgtcgg ttttacgacc aattacccgt taaatttgat catgactggt
    35461 gcggcgtcac gcagttaggc tggaatgaga aaagccagca taaaatcgca cagatgttgt
    35521 caatggattt acccgcagaa ctggctgtag ccgttgaggc aaatgcggtt gaacaaatta
    35581 cgggcgttgc gacaaattgc agcggcatta cttatccgca aggtggttgg ctgtgcccag
    35641 cagaactgac ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag atttattatc
    35701 aatatcagtt acagaattta tcccgtaagg atgactgttg gttgttgaat tttgcaggag
    35761 atcagcaagc aacacacagc gtagtggtac tggcgaacgg gcatcaaatc agccgattca
    35821 gccatacgtc gactctcccg gtgtattcgg ttgccgggca ggtcagccat attccgacaa
    35881 cgccggaatt ggcagagctg aagcaggtgc tgtgctatga cggttatctc acgccacaaa
    35941 atccggcgaa tcaacatcat tgtattggtg ccagttatca tcgcggcagc gaagatacgg
    36001 cgtacagtga ggacgatcag cagcagaatc gccagcggtt gattgattgt ttcccgcagg
    36061 cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc gcgctgcggt gtgcgttgtg
    36121 ccacccgcga tcatctgcca atggtaggca atgttcccga ttatgaggca acactcgtgg
    36181 aatatgcgtc gttggcggag cagaaagatg aggcggtaag cgcgccggtt tttgacgatc
    36241 tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc tgccccgctg tgtgccgaga
    36301 ttctggcggc gcagatgagc gacgaaccga ttccgatgga tgccagtacg ctggcggcgt
    36361 taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg taaagcggtt aaggcggggt
    36421 aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc cttatccgac ctacgtgtga
    36481 c
//
LOCUS       DAPHDX010000039        33232 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPHDX010000039 DAPHDX010000000
VERSION     DAPHDX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.039"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(362..652)
                     /locus_tag="RH058_001828"
     CDS             complement(362..652)
                     /locus_tag="RH058_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3137420.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(653..964)
                     /locus_tag="RH058_001829"
     CDS             complement(653..964)
                     /locus_tag="RH058_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3137421.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1193..2101
                     /locus_tag="RH058_001830"
     CDS             1193..2101
                     /locus_tag="RH058_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3137422.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2165..3106)
                     /gene="fabY"
                     /locus_tag="RH058_001831"
                     /gene_synonym="yiiD"
     CDS             complement(2165..3106)
                     /gene="fabY"
                     /locus_tag="RH058_001831"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV3137423.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3151..3588)
                     /gene="dtd"
                     /locus_tag="RH058_001832"
     CDS             complement(3151..3588)
                     /gene="dtd"
                     /locus_tag="RH058_001832"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV3137424.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3585..4457)
                     /locus_tag="RH058_001833"
     CDS             complement(3585..4457)
                     /locus_tag="RH058_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV3137425.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4451..5050)
                     /gene="yihX"
                     /locus_tag="RH058_001834"
     CDS             complement(4451..5050)
                     /gene="yihX"
                     /locus_tag="RH058_001834"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV3137426.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5191..6078)
                     /locus_tag="RH058_001835"
     CDS             complement(5191..6078)
                     /locus_tag="RH058_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV3137427.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(6068..7417)
                     /locus_tag="RH058_001836"
     CDS             complement(6068..7417)
                     /locus_tag="RH058_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV3137428.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7516..8949)
                     /locus_tag="RH058_001837"
     CDS             complement(7516..8949)
                     /locus_tag="RH058_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137429.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9019..10029)
                     /locus_tag="RH058_001838"
     CDS             complement(9019..10029)
                     /locus_tag="RH058_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV3137430.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10062..10949)
                     /locus_tag="RH058_001839"
     CDS             complement(10062..10949)
                     /locus_tag="RH058_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3137431.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10974..11852)
                     /gene="yihU"
                     /locus_tag="RH058_001840"
     CDS             complement(10974..11852)
                     /gene="yihU"
                     /locus_tag="RH058_001840"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV3137432.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12025..12921
                     /locus_tag="RH058_001841"
     CDS             12025..12921
                     /locus_tag="RH058_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3137433.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12961..13749
                     /locus_tag="RH058_001842"
     CDS             12961..13749
                     /locus_tag="RH058_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3137434.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13860..15125)
                     /locus_tag="RH058_001843"
     CDS             complement(13860..15125)
                     /locus_tag="RH058_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137435.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15227..16207)
                     /locus_tag="RH058_001844"
     CDS             complement(15227..16207)
                     /locus_tag="RH058_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV3137436.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(16215..16925)
                     /locus_tag="RH058_001845"
     CDS             complement(16215..16925)
                     /locus_tag="RH058_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV3137437.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17142..18965)
                     /gene="typA"
                     /locus_tag="RH058_001846"
     CDS             complement(17142..18965)
                     /gene="typA"
                     /locus_tag="RH058_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV3137438.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            19338..20747
                     /gene="glnA"
                     /locus_tag="RH058_001847"
     CDS             19338..20747
                     /gene="glnA"
                     /locus_tag="RH058_001847"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV3137439.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            21066..22115
                     /gene="glnL"
                     /locus_tag="RH058_001848"
     CDS             21066..22115
                     /gene="glnL"
                     /locus_tag="RH058_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV3137440.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVLDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22127..23536
                     /gene="glnG"
                     /locus_tag="RH058_001849"
     CDS             22127..23536
                     /gene="glnG"
                     /locus_tag="RH058_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV3137441.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23648..23758
                     /locus_tag="RH058_001850"
     CDS             23648..23758
                     /locus_tag="RH058_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV3137442.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23948..25321)
                     /gene="hemN"
                     /locus_tag="RH058_001851"
     CDS             complement(23948..25321)
                     /gene="hemN"
                     /locus_tag="RH058_001851"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV3137443.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25510..26019)
                     /gene="yihI"
                     /locus_tag="RH058_001852"
     CDS             complement(25510..26019)
                     /gene="yihI"
                     /locus_tag="RH058_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV3137444.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            26601..27233
                     /locus_tag="RH058_001853"
     CDS             26601..27233
                     /locus_tag="RH058_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV3137445.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27615..30401)
                     /gene="polA"
                     /locus_tag="RH058_001854"
     CDS             complement(27615..30401)
                     /gene="polA"
                     /locus_tag="RH058_001854"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV3137446.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30765..31697
                     /locus_tag="RH058_001855"
     CDS             30765..31697
                     /locus_tag="RH058_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV3137447.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31738..33168)
                     /locus_tag="RH058_001856"
     CDS             complement(31738..33168)
                     /locus_tag="RH058_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV3137448.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8371 a         8542 c         7949 g         8370 t
ORIGIN      
        1 gcataatctg aatggtgtac ccgaaaaatg tcagcatcct aacagcaccc ggatttaagg
       61 tgaaattaat catccatcac agaccggtca gtactgccac caaccggtct ttacttgttc
      121 atcgttataa ttttgttggt aacagttcgg gaaacaagga attccttccg gaaccccccg
      181 gtatcatccg atagcattga cgtcaatcat cagcgcgacg atgagttttt ttaatcgcat
      241 catagggtgg cgacgttcag cacgattacg ggtcatagca aaactcctgg gcaggcgacc
      301 actatgccgc tttcacagga gttctgaatg ctagtgcttt ctgtatggca taaccatgcc
      361 tctaacggtt aagtgcttgc agcacatgtt gcggatcatt ggcaatcgct cgcagcaaag
      421 cctttgcagg cccggtcggt gaacgtcgcc cctgctccca gttacgcaac gtgtctacgc
      481 ttacagaaat aagatcggca aatttagatt gtgacaaccc tgaagcgcgg cgtatttctt
      541 tgattttcat agcatcaact tcaaaagtcc gcgaaggctg tcgcgttcct tcaataattt
      601 cattcatctg ggccatgctg tgagtcaacc gctcaaaaag tgccttatcc atttaccacc
      661 tcgtatttaa caaccggagc attgcttttt cctggggaga cagatcatct ttaataccct
      721 tacgataaat aagcagtaat ctgatctcat aatgtttaac ctgatgaaag taaataaccc
      781 tgacaccagc acgtttcccc tttcctcctg aaacccatcg aacctttctt aacccaccag
      841 tttcaggtat tacctcacca tagtcaggat tcaaggccag gaaaaactga aaacgactga
      901 attcatcgtc ggtcagcagt ttttgaacat cttcagtaaa aatttccgtt tcgatgaaga
      961 gcataattat acgccattgg cttagagtaa caatcctttt tgtacgtcat tgacgtaccc
     1021 ttgaaaccac tagcttactg atagtgctcc agcaaaacca cgtctctgcc gggtttgtct
     1081 ttagatttgc gaggcgcttt ccagattttt cacgcccgtg aatttccctg tatccatcca
     1141 gcaaaaacgg gtgtataaga ctgtacattc atacagcaaa ataagttcta ctatggcact
     1201 ggaaaaagga attgcagagt tagtcgaaaa gtttatcgct gcgggtcgcc cctcatcgcg
     1261 tcaacaaagt attgcgcagc gcagggaggg ctatatcgcc agcgctgtcc ttgcgggtga
     1321 gacagaaacg cgggtagata ttcagactat cgaacttgaa gggatgacct tacgaatagt
     1381 gtcgccgctg aatgccccga cattactccc gacgataatt tattactacg gtggctgttt
     1441 tgttagcggc gggttcgcaa ctcacgacaa ccagctgcgc cagttagcct attacgggca
     1501 atgtcgggtt attgccgtcc agtatcggct ggcaccggaa catactttcc ccgccgccca
     1561 tgatgatgcg caacgaggtg cagaaatagt ctggcaacac gccgaacgac tcggcgttga
     1621 taaacagcgg ataactcttg ccggtgacag tgcgggcgga catctggcgc tggtaactgc
     1681 tttgcgatta aagcgtgctg gcgagtggca accggcacaa ctcatcttaa tttatcctat
     1741 gcttgatgcg actgcgcatt tcgaaagcta catacgcaat ggtcacgatt acatcatcac
     1801 acgcgacact ttactgagcg gctttgagat gtatctgcca ggcattgaac gcaggcatcc
     1861 tgaagccagc cctctttggc gcgatgattt caatggcttg ccacctgtac atatcattac
     1921 cgctgaatac gacccacttt gcgatgaagg tgaagcgttg tatcaccgca tgacagggca
     1981 aggagtgcaa tgcacctgcc aacgctacct tggcgttatc cacggatttt ttcagttagg
     2041 tggtatcagc gaggccgcgc gatcggcact acgggatgtt gcctggagag cgggaaggta
     2101 acttcggacc tgggccctga aaaggttttc agattgaaaa ccttttcagt caagtaaaac
     2161 gcccctactc ttcttcgttc ccgccctctt catacgggcc aaatggcttc gcgggcagaa
     2221 cgatatacgt gccttcaaac actgcacccg gcgtctcgtc gccaaagatt tcaacttgca
     2281 tctgcacccg tgcttttcgt ccgcgcgcca gacggtcgag atcgccgctt aaggcaccaa
     2341 ggtcggctac cgcatgaggt ttaccgctaa tcggcttgct gtagcggata tgcgcatccg
     2401 ccagaataat cgttccgccg agatggcgtt cgcgcagcat cagccagata agcccccaac
     2461 cggtgagcgt cgccagcgag aataaactcc cggcaaacag cgtatggtgc ggattctgat
     2521 tgccggtttc cggcatggtg gtgataaatt tttgcccggt atattgttga atacgcacgc
     2581 ccattttttc actaagcggg atatgttcgt accacgcctg ttgcagctgc gcgcaccagt
     2641 cgccgcgatg caaaatatca tccagagtgg cgacgggctt aatcatcaaa aaatggcgaa
     2701 tcggcgtggt ggttggcgtg gtgatttctc cctgattaac aaaccccagc ttggcgaaaa
     2761 actccaccgc gtcttcacgg gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca
     2821 ccgactccag ggtcatcgcc atcagcgttc ctaacccttt gtcctgcacg tccgggtgaa
     2881 cggccataaa gcgaatggac gcttcattgt cggcattaat atacagtcgg cctaccgcca
     2941 ccagattacc ctgctcgtcg acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc
     3001 gttccgaacc ttttggttga tgcaggggct tacgcaacat ttcccagcga aactgatagt
     3061 aacgctctaa ttcttcttct gtttgtggaa cccgaaggtg atacatagcg atactctctc
     3121 ttgtttcccg tgacaaccct ggaagctggc tcatacctgc aaccagaatg tcacggggcc
     3181 atcattgacc agcgatacct gcatatcggc agcgaagcgt cctgtttgcg tgttcatctc
     3241 ttgctggcgg cagcgttcga cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc
     3301 gcctttggag aaacttgggc gcatccctcg ttcggtatct gcggcgaggg taaactggga
     3361 aaccaccagt acactgccgc ccgcctgttg cacgttgaga ttcatcttgc cttcggcatc
     3421 gctaaagatg cggtagccga gcacacgctc gcacagacgg tttgctttct gttcgtcgtc
     3481 atccttttcg acacccaata acaccaaaag tcccgcgcca atttcgcccg tcacttctcc
     3541 ctccacggtg acgctggcac gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct
     3601 tcttcttgtt cagctgcttg ttttagtttg cggtattccc cgagagtgac agtaatttcc
     3661 gcgccaagca agacgataca ccatgtccag tagacccaaa caaagagaat ggggatcacc
     3721 gccagcacac cgtaaatgag ctgatatgac gggaacatgg tgatataaag cgcgaaacct
     3781 ttctttcctg cttcgaacag gagcgcggcg acaaacgcgc cgacaatcgc gtcgcggtta
     3841 ggtacgcgga tggtaggaac aatgctgtac agcaaccaga aggagatcca cgacaacagc
     3901 agcggaaaaa tacgcagcac gttgtcgatg acagtattga gatcgctcgc ccagcgcagg
     3961 gagagcaaat aggaactgat cgccagactg gcccctgcca gcagcggccc cagcgttaaa
     4021 atcatccagt acacggcgaa cgagtaaatt tttggtcgcg ctcgtttact gcgccagatg
     4081 gtattcaacg cgctatcgat ggagtacatc agcaataacg cggtgacgat cagcccgcac
     4141 gccccaacgg cggtcatctt gttggaattg gcaacaaatt gttcgatata ccgctgaata
     4201 acatcgccag tagcaggcag aaagttggca aaaataaagt gacgtaactg aatgctgacg
     4261 tcggaaaaca tgggaaaagc ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat
     4321 gagagcaacg acacataggc aaggttacct gccagggttg tcatgttgtc ctcatcaatg
     4381 cgttgccaga gtagttttag ccaggcccat agtggacggg tacggtgcct ggctttgtcc
     4441 tgaatggttt ttagcataac accttcgcga aatagtccgg gatggtggtt ttatctttca
     4501 ccagaatact ggtaatgccc agctgattgg ctccttctat attatcggcg ttatcgtcga
     4561 aaaagaccgt atcgctgggt gaaaaacctt ccgcctgcaa aacatgctgg taaattcgtg
     4621 cttcaggttt gcgcatcccc agatcttgcg acagatagat atggtcagca gcatcacgaa
     4681 tttccgggta ttcttccggc cagaaggtgg tatgcaggcg gttggtattg gaaagcacca
     4741 ccacgcgatg cccctgctca cgcagtttat gcatgatggc gatcacttcc gggcgcagcg
     4801 caacaaacac cgcctgccag ccgtgagaga actgctcgta gcttagcggt agagccatct
     4861 catgacacag cgcctctgcg aacgcttcgt cgctaatttc cccacgctca tgctgatgaa
     4921 acgcctcccc catatgaaaa ctcttcttaa gcgatgccag cggaatacgc gttaaatcgc
     4981 tccaggctcc cagcacacgg ttaaagtcga tatcgacaat cacattacct aaatcaaaga
     5041 tatagagcat ttttgcctcc tttcgcgcca caagaaatta actgtagcgg gaaaggatgg
     5101 gtttggctat gtggcaataa gggtaatctt gtgatgctga ttgcgaaaaa gcagagtaat
     5161 ttcactggct ttttcgcaat agatgaatgg ttaagattca ggtgtaaatt gccaacgagt
     5221 ttcgatgacc agcggcctat ctggtgtcac caattccggc gttagattta ttgcgttagg
     5281 tgcctgagtc agcgggttaa tacaggtggc atcgggctgt ttatcaaata ccaccagact
     5341 gtttgcagaa gagatcatgg tcatcgcgag tttaccaggc cacagtaact taaccttcac
     5401 cccgtcataa aaaccaaaac agtcatccca gggaccggct tgaggagaga tacgatttcc
     5461 agttggcagt tcatcactac ccgcttcttc ttgccagtcc gcatcaaaaa gcacctgcaa
     5521 cgattctgta ttttgcggag tcagcttttt cgcaaaccac ggatgccagc ctgcggaggc
     5581 cgggaatgta tcggcatatg aatggacttc cagttgcaaa accagcgcat cgttctccag
     5641 caaaaaagtc tgaatcactt ccccttgcca gggccacggt gaagccaggg gcatacgtaa
     5701 agtcagggaa tctgctgttt tgtcgatgat ttcccacgtt gagtaacaag ccataccatg
     5761 caacgcatgt ggtggtttat tggcgggtaa agaataacat tgcccaccag cattcagcgt
     5821 cgcattcccc agtcgccctg cccacggcac cattgggaaa cagccatact gaaaagctct
     5881 gcgctgcggc tgccattggc gtaatacttc agaaccaaat gcctttaacg aggttatcct
     5941 ggcaccatca tgcggataaa tctgcaggtg aatattgcct ttttccagcg tcactaactc
     6001 tcctacaggc ccaggccatg gcgcgtcaaa cgcctgttgt agcgccaggt aatgctcgtt
     6061 ttttcgttca gttttcatga gattgttccc tgatgtagcg gtaagaagcg gtttcttgtt
     6121 catcggtcgg ctcaatcagc cagacgcaaa caaaggaaag aactgagaca gcaaataaaa
     6181 acagagcgac ataccacggt tttcctcccc ctagcgccag caaacttgag gcgataaggg
     6241 ggcttagtcc gccaagaatc gcggaaaatt gataagcaaa agaaaggccg gtatatcgta
     6301 ctttggtacc gaacatgcgg gtgaaaagtg ttggctgcac ggcaaacatc atcgtcggac
     6361 ctaagttgta gccgaggatc atactgcacc agatgatgag cgtacttttg ctatccagta
     6421 ataagaagaa aggaaacgcg aacaggacac aaaatcctgc tcccgacaaa tacagacttt
     6481 tttgacctat acgatctgat aaccatccaa ccaatggaca actgaaaata ccgatcgccg
     6541 aggcaatcaa catacccgtc aggggaatat ctcgcgacaa tgctaattgg ctggaaatat
     6601 agacaatacc aaaggcgtta ataatattag aggagacact ttccgccagc ctcgcaccca
     6661 gtgccaataa aatatttcgt ggatgttttt tgaataattc aatcaacgga ggaacggact
     6721 tttctttatt attcgtcgtt ttttgctttt gaaaatccag cgtttcttcg gtatgcaggc
     6781 ggataaacag cccaacgatc aacatcaccg cagaaagcca aaacggaatg cgccatcccc
     6841 aggagagaaa ctgctccgaa gtaagaaaat ggttacagag agcaaaaata ccggttgcca
     6901 gcataatacc cacagacgca gccgtttgcg ggagtgaacc taaaaatccg cgtcgggatt
     6961 cgggcgctga ttctatggtc atcaatgccg ctccgccata ctcgccacca agaccaaagc
     7021 cctgaattaa acgcagcgcc attaatatga gcggtgccca gatcccaatt tcctgatacg
     7081 tcggaataaa accaatcaaa aatgtggagc aaccgacaat agccagtgtc cagataagtg
     7141 ttatttttcg cccaatttta tcgccaaaat ggccaaagac gataccacct aaaggacggg
     7201 tcaaaaaacc aacgccaaag gtagcaaagg cggctataag acctataaaa ggatcgctgt
     7261 ctggataaaa cagcggtgca aataccagtc ctgctgccgt gccaaaaata aagaagtcat
     7321 accattccat taacgcgcct gcgaaactgc ccataacgac acgtctcatg gctttaccgg
     7381 aaagcccttc acgcggtgcg ggtaactttt cagacataat tttcctctcc tggtaggcga
     7441 gaaatctggg atacgaacca ctaaacgtgg gcagtttgaa ttaatgcgca tcggtttaag
     7501 ccgatgcgca ttaatttatt tcttcaattc agccagaacc tctgcatcct cagcatcaac
     7561 atttgcaccc agtggttccc aattaatcaa tacgcaggtc aggaacccca agaatggcac
     7621 gattgccaac cagtaaaacg cttcagtttt caacgccccc cacagcatcg gccagaaaat
     7681 gagtcctgca attgcccctg tgcgcataat agcgcgggca aatccgaccc cagtagggcg
     7741 aatagcggga ggataagaga gtgtggcaat ggtcatgccc agcccaccag gacccgctga
     7801 atggaagaaa ataattgatc ccaataaccc taaagacagc catggattag tcgttgccag
     7861 tgcgcctaac gacagcaatg ctaaggtcac aacagcaaat ccatacatcg actgtcggcg
     7921 agtacccagt cgttggagaa taaacgagcc gatcatcccg cctgtcaccc cacaaagggc
     7981 attcacgacg gcagaacccg tcaacgcacc agtaagcccg ccacttatta ttcccgccaa
     8041 cgtaagagga agataaacac ctacggcgtt atattgccac gcctgcatgg ttgccacgac
     8101 acagccaaga atggtgcgtt tgcggtaacg atcattaaat aagatgccat atccaccact
     8161 gtatttattc tctgcttttt ccgagaattt cgcttctgtt gtactcgatg ccgcaacgcg
     8221 agcctgaaca ccataacgtt ttcccagaat gtcgcacgct tcctggtaac gcccgacgcg
     8281 tgcggcccac attgcggatt caccaataaa gaagtaacgc aaaatcatga agatgactgc
     8341 aaaaatagct ccgatgaata ttccgtaacg ccacaattgt tcttcagcga tacccgacag
     8401 aagcagcggc aagagcacca aataaacaac agttgtcgaa acataccagg ccatttgcca
     8461 gaggttgacc gacgttccgg ttttcttcga cgttgaacca cgaagctctg ccacggcatt
     8521 ggttgccaaa gggaagtcga tgcctaaacc aaagcccatg atgacgcggc acaccagcac
     8581 ccaccagata ctgggcgcaa tagctaccgc agcagccccg atggtgcaca gccccatgcc
     8641 aataataaat gctcgctttt gcccaaagcg gtttattact cggttcgcta ataaaccgcc
     8701 aattaacgcg gcgattaata ctgacgcatt aacggttgcg gcaaggcctg gagaaatgcc
     8761 aaattgagcg gcaatgtatt tattaccaaa ccccaccatc gccgcctggt aggcatcaat
     8821 caatatgccg cccaaagcaa taaaaataac gccgatactg gtgttgattg cagagttctt
     8881 attaacaata tcaattacgt cctgtggccg actaattgaa taagttgcag gactggtgat
     8941 ttgactcatc gcagtgtcct ttttaattgt tgtagggtgg cgctgacgtg acgcagcgcc
     9001 agacagagat ttagaaaatc attccggcgc aaattgcagc atgaccttta tggagtcatt
     9061 ttgctgcgcg cgttcgaatg ccgccgctgc atcctcaatg gcaaataaat cggtaaccag
     9121 ttcactggta tcaaaccgcc catccgccag ccattcgata acagcatcga aatcttcagg
     9181 gatgtacatg ccagagttga gcaagtcacg ctcgaaacgt tgcatcatcg gtaatggtat
     9241 ttcacgcggc ccacttggta cgcccatgca cacgacggtg cctccggcat atacacgggt
     9301 acatgcatcg ttaagcgtgg cctgcgctgc aacgacatca atcgcccccg taaatcgcac
     9361 atctgcggcc agctcacctg gtgcccaaac ttctgctgcg cccagcttta acgccagcgc
     9421 gcgtttggca gcgtcaggtt caatcacggt aatcttacct gcccccataa tgcgcagtgc
     9481 ctgcacgatg gataagccta tcgttccggc accaatcacc agtacgcttt ccagagaagc
     9541 tttcggcatt cggttaacgc agtgacgggc acaagccgcc ggttcggcaa aggccaacac
     9601 ttttagcggt aaagaggctg gtgcgacatg gcaattacgc gcaggaacaa tgtgttgtga
     9661 gcgagcaaag cccggtgcgc gaaaaccagc aacctgcggt ggttcacaaa gattaaaacg
     9721 tcctgctttg caggctcggc attccatgca agccatgatg ggatcgacca caacatgatc
     9781 gcccggctgt acatttttaa cgtcgctgcc aacttccgta acgcgcgctg caatttcatg
     9841 accggggacg acaggtggtt tagcaaacgg atgaccgcct ttcagaacat gcagatccga
     9901 accgcatatc ccatagaaca caggcgcaat ccgcacttcg tgcggtttaa gttcctggtg
     9961 tggtacttca acatattgag tggtgacttt cccaatatct gaaaataaaa cctgggtaat
    10021 tttatccatt gttattctcc agaatcgata tccatagggg attagcgttg cgcgatacct
    10081 tcatcgacaa tttctgccaa ccgttgtaaa cgaggaacag agacatcgcg cagcatagtt
    10141 tgtggatctt gtgcaccaac aaccgacgcc caaaccgctc gtccagccag gaagcctgac
    10201 gcaccacctt tcatggcaat acttactgca cggccaaaaa tatcggcatc aacgccagaa
    10261 gaaagaatta cccacggcat cttcatctga tcgttaagtt gctgacaggc ggcaagtaag
    10321 gttttttcat cgcctttgcc ccctaatggc atctcggctt tgtaaagatc agcctctgtt
    10381 ccaccaagct ctgcggcggc ggcaacaatg gcgctttcac gatcaaagtc ccaaccgcga
    10441 cgaggtggac gcaccacagg ttcaataatg ctaaccagcc cggcactacg gcagcgacga
    10501 acaaatttat caaccattgc cagacgttcc tctgcgggtt catcttcacg ccagagtaca
    10561 agtaatttca ttgccttagc ccccatttca cgggctttat gcgggtcgac ggacatatct
    10621 atttcaacgc tatcaaccgg gatgccatta cctgggataa aaagatcggc agcgacaatc
    10681 aggccgcaag aattagcaac agcgccctgc tcgactattt gctcaagaca aaattgttta
    10741 tccgccagaa cggcagaagc ataaggagaa aggattcggg tcgcggcaac tttaaaatcg
    10801 gtgagtacac tgtcggcgat gggttttggc tgccctgctg ccgcaaacat caagcgcatg
    10861 gcctcacgct gatcgacagc taacatggca aatccaccac ctgggcggga gatatctttc
    10921 agcgtaaatg gggtgaagtg ggatgacata gaaactcctg ttaatagata aaattaaaga
    10981 ccagcctgct ggcgaacctg atttagcagg gcactccagt cctgccgccc tttgccgtct
    11041 tgacttgctg cagcgtaata actttctgct gcaatcccca tattgagcgg tgcccccgtc
    11101 tcccgagcga cgtcaactgc aatacgcaga tctttcaacg ccagatccac catgaaggcg
    11161 ggtgaaagat cgcctttcag cactttgcaa ggccaggtgg tcgtaaaatg tcctttccct
    11221 gctgccgtac cactcatcac cttaattgcg acatcaagat ttaatcccag tgactcacat
    11281 aacacagcgg cctcggagga cagcgcatta agcgcaatgc tcatatagtt gttaattagc
    11341 ttcacgcgga tccccattcc tgggccgcca gcctcgacca gctcattacc catgcacatg
    11401 agaatcgggc gtgcacgtgc aatctgctca tcagtgccac ccgctaaaat cagcaatgtg
    11461 ccatcaatcg cattcacgga cgttctgccc acgggggcgt ccatcatact gatgcctttt
    11521 tcttgtaact cccgtattaa tgcatcggtc tgtaagggat gaatagtcga catatcaatc
    11581 aataacgcgt cagatgaaac agcctcacag acgccttttt cccccaacag aacctgtcga
    11641 acaatgtcac cattgggaag catagtgatg ataaattcag cgcctgctgc cgcttccgct
    11701 ggggtttgtg ccgcagtggc cccctgcgta actagcgcat cgacggcctg tgcattgaca
    11761 tcaaatacct gcaatgaatg ccctttttgc aatagattgt tagccattgg gctacccatc
    11821 tgccccagcc cgagaaatgc aatggttgcc atgaatactc tcctgtctga cattgacaaa
    11881 ataatgacaa ttgaaaattc gttttcgtct gtttttgagc atatttgtaa tttatgttca
    11941 aaaacacgac acgcatcact aatttgatca tttgtgtcat ttaaattgac cgaagacgac
    12001 aaaattaaac aggacaagac aaatatgacc aaaattgcat gcgtgggtat cacagttcag
    12061 gacaggattt attctttgct cactctgccg gatggcggcg gaaaatatca ggcaaatcat
    12121 tatcttgaga ttggtggcgg cccggcagca acagccgctg ttgcaatcgc aaagcttggc
    12181 gtagaggttg atttcatcgg tcgcgtcggc gatgacagtt gtggaaatac cttacttgca
    12241 gaacttgagg gttggggcgt caataccgca ttttgccgcc gatatcctaa cgcccgttca
    12301 tcgcaatcag cgatcctcgt tgatcagcat ggcgaacgga ttatcgttaa ttatccaagt
    12361 cccgatctcg gtactgatgc cgaatggtta gaagcgatcg atttctctcg ttatgatctg
    12421 attctggccg atgttcgctg gcatagcggt actgaaaaag cattctcgct agcgcgtctt
    12481 gcgggtgtga caacgctttt agacgccgat atgacaccgc aggatatttc tccacttgtc
    12541 gcgcttgccg atcacgccgt gttctcaaca ccagggctga aacgcatgac tggcctgcag
    12601 tcacctgaag aaggtttgtt ccaggcaacg acacaaacag caggtaaagt gtatgttacg
    12661 ctgggttctg aaggatcgtt gtggattgaa gatggtcatt tgtgtcaaca ggaggcattt
    12721 tctgtcaatg tggttgatac cactggagca ggcgatgttt ttcacggcgc actcgcggta
    12781 gcgttggcag aaaaaatgcc aacaaaagag gctattcgtt ttgccagtgc ggttgccgcg
    12841 atgaaatgta cccagcctgg cggtcgggcc ggcattccta atcgtgagca aaccgaatca
    12901 tttttgtcac tttatgcgta aaatggtaaa catctaccga ggaggggcaa gggagtattc
    12961 atgagtatca tcgaagtgac aggtaatcca cgccatgacc aactcgttca tctaatcgca
    13021 gaacgcggtt atatgaatat tgaagaactg gcacaactgc tggatgtttc aacgcaaacc
    13081 gtgcgccggg atatccgaaa actcagtgag cagggcttga tcacccgtca tcatggaggg
    13141 gccgggcgcg tctccagcgt catgaatacc gcttttgagc aacgggaact ttcgctcacc
    13201 gccgagaaac gggcgatcgc tgaggcagtc gccgattacc ttcccgaacg ctgtaccgtc
    13261 tttatcacca tcggtacaac cgtagaagcc gttgccaggg cattactcaa ccggcgtgat
    13321 ttacgcatta tcactaacag cctacgtgtg gcacagattc tttataagaa tcaggatatt
    13381 gaagtgatgg tgccgggagg cactttacgc gctcataacg gcgggattat cggcccagga
    13441 gccgtggatt ttattgaagg cttccgcgca gattatttaa tcaccagtat aggtgccatt
    13501 gaacatgacg gcaccctact ggaatttgat gtaaatgaag cgttagtcgc gagaacgatg
    13561 attaaacatg cgcggaatac attgttagtg gccgatcata cgaagtttgc cgcgtctgct
    13621 gccgtttcaa ttggcaatgc acggaatgtc agggcttttt ttacagatgc cccgcctccc
    13681 aattctttct gccagttgtt aagtgaagag aatgttgaac tggtggttgc cgagcaggaa
    13741 gtttcctgaa attgaaactt cgaaaaatcc gctggggatt cagcggattt tattttgccg
    13801 gatgcggcct acggttgata cggttttcgt tggccggatt aagcacatcc ggccattaat
    13861 taagcttcag ccgccagcat ctctttctta cgttggctaa tacgcttaac cagcattacg
    13921 gaaaccaccg caccacagaa tgcgaagcag gccataatgc caaacacgat tttgtagcca
    13981 ataatccccg gatttaaatc cagaatgtag ccatacagac tgaagcagaa catcgccgga
    14041 gcgtaaccaa taaagctacc caacgccatc gccgcgcctg ttttattttc agcaattttt
    14101 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
    14161 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
    14221 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgattttagg
    14281 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
    14341 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
    14401 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttaaa agcgataagc
    14461 cagatggttt tgtctctcag caccgaggtc atactggtgt tttgcgatgc tccgtcttct
    14521 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
    14581 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc caataagcca
    14641 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
    14701 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
    14761 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
    14821 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
    14881 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
    14941 agaaaaaaac ccactgtcgt gacaaatgag tttaccgaca tagcattacc aatttgacca
    15001 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
    15061 ttataaattg taccgccaca caatgttaat agactaaata acgcccattt ctttttcgtg
    15121 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
    15181 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
    15241 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
    15301 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggcaatggtg
    15361 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
    15421 ccggcgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
    15481 cacccaggca tataaggtgc gttaataacg ctttgaacgg catatcaccc tgacctgaaa
    15541 tacaggcttt atgacccagg ccacccggtt ctttaacgat caaggcatct ttgatatgga
    15601 cctgggtaat atgcggcgcc atcgttttta aagcgtcgat gggatgctca tttgcattga
    15661 tcatgttcgc aaaatcaaac agtaaggaaa gagattccat ctcactttct ttgaccagcg
    15721 acaccagctc atggcttttt aaatcttcat gctgttcgat cgtaaaagtc aggccgctgt
    15781 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat gatcgataat acgtcgcgca
    15841 aattaccttc ataacgtgga taaaaacgta cggacgatgc cccagttttc aacgcaatgg
    15901 cgacggcttc gtcaatagat gccttatctg aggcgctggt ttcaatatga atatcaaggt
    15961 tcagacggcg ggctttatct ccaaaggcag agagttcttt atcgtccata ttaccaagag
    16021 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga
    16081 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
    16141 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
    16201 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
    16261 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
    16321 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
    16381 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
    16441 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
    16501 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
    16561 gatggtgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
    16621 tcggtaaacc cccaggaagg ctctcgtcct tgctcaattg cggcccgctg aaagctcgcc
    16681 gacaactccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
    16741 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
    16801 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
    16861 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
    16921 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
    16981 gaaagcaatt aacggcgtat gactttgcag ataattgtga caaagctctc tattgacgta
    17041 atcccagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
    17101 cctgccagcg atggcaggtt ttttataact aaaaaacgaa attaatcgtc tttcggtgcg
    17161 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
    17221 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
    17281 cggataggcg gaaccagaac aacggcttcg tcagtaccgg aagcacgcat gttggtcagt
    17341 ttcttaccgg tcaggcagtt taccgtcagg tcgttagagc ggctatgaat accgataatc
    17401 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
    17461 aacagcgcga acgcgaccgc tttaccctga ccgttagaga tcagtacgcc gttctgacgc
    17521 tgacccactt cacccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
    17581 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
    17641 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
    17701 cgttcgccca gcgcctgcat tacagaaccc tgatgctgtt cttcaacgtc cagagtcacg
    17761 ttttcatacg gctcttgttt acgaccgtcg atttcacgga agataacttt cggacgggat
    17821 accgccagtt cgaaaccttc acgacgcatg ttttcgatca gaacagacag gtgcagttcg
    17881 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
    17941 ttgtgtacca gttctttgtt cagacgatcc aggatctgac gagacgttac gaacttacct
    18001 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
    18061 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
    18121 agttcgccaa ggcccgtgat cgcaacgata tcgccagctt ccgccagatc ggtttcgata
    18181 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttgcgggt tttgccttcg
    18241 ctatcgatga tagtgacctg ctggttcggc ttcactttac cgcgcttgat gcggccaatg
    18301 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
    18361 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctggtacag cggggtcatg
    18421 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
    18481 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
    18541 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
    18601 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
    18661 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
    18721 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
    18781 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
    18841 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
    18901 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
    18961 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
    19021 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
    19081 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaacatggt gcttaatgtt
    19141 tccattgaag cactatattg gtgcaacatt cacatcgtgg tgcagccctt ttgcacgatg
    19201 gtgcgcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
    19261 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga ccatgaccaa
    19321 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
    19381 tgaagtttgt tgatttgcgc ttcaccgata ctaaaggtaa agaacagcac gtcactatcc
    19441 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
    19501 ttggcggctg gaaaggcatt aacgagtccg acatggtgct gatgccagac gcatccaccg
    19561 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
    19621 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
    19681 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
    19741 tgttcgatga catccgtttc ggatcatcta tctctggttc ccacgttgct atcgacgata
    19801 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
    19861 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
    19921 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
    19981 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
    20041 aaattcagat ctacaaatat gttgtgcaca acgtagcgca ccgcttcggt aaaaccgcga
    20101 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
    20161 tgtcgaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
    20221 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
    20281 cgaccaccaa ctcttataag cgtctggtcc cgggctatga agcaccggta atgctggctt
    20341 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
    20401 gtcgtatcga agtacgtttc ccggacccgg cggctaaccc gtacctgtgc tttgctgccc
    20461 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
    20521 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
    20581 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
    20641 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
    20701 gcatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
    20761 actacccggc gcaacaacgc cgggggttcc aaccaggccc atctggcgta attgttgcag
    20821 ccagtttgaa cacggacagc gcgcagaacc cggagcgtac actggtacgt gaggagttcg
    20881 agcactgccc aggttcaaaa tggcaaataa aatagcctga tgggactggt ttttagttgc
    20941 cgtggaaatt tcagcccatc tctgcatggg cttttttctc cgtcaattct ctgatgcttc
    21001 gcgcttttta tccgtaaaaa gctataatgc actaaaatgg tgcaacctgt tcaggagact
    21061 gctttatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg ctgattaaca
    21121 gtattttgtt aatcgatgac aacctggcga tccattacgc caaccctgcc gcgcaacaac
    21181 tgctcgccca aagctcccgc aaattgtttg gtacaccgtt accggaactg ttgagctact
    21241 tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggt tttaccgata
    21301 acgaagtgac gctggtcctc gacgggcgct cgcatattct ttctgtgacg gcccagcgta
    21361 tgccggacgg catgatcctg ctggagatgg ctccgatgga taaccagcgc cgcttaagtc
    21421 aggaacagct acagcacgcc cagcaggttg ctgcccgtga tttagtgcgc ggcctggcac
    21481 atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagctactc agcaaagcgt
    21541 tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg gaccggctgc
    21601 gaaatctggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt accgaaagta
    21661 ttcacaaagt agctgaacgc gtggtaacgc tggtgtcgat ggaactgccg gacaacgtgc
    21721 ggttgattcg tgattacgat cccagcctgc cggaactggc gcacgacccg gatcaaattg
    21781 aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg gagggtggtg
    21841 aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag cgctaccggc
    21901 tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat ttgcaggata
    21961 cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc ttatccatcg
    22021 ctcgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg ccagggcata
    22081 ccgagttctc ggtttacctg cctatcagga aataaaggtg acgtttatgc aacgagggat
    22141 agtctgggta gtcgatgacg atagttccat ccgttgggtg cttgaacgtg cgctcgctgg
    22201 ggcaggttta acctgtacga cgtttgagaa cggcgcagaa gtgctggagg cgctggcgag
    22261 caaaacgccg gatgtgctgc tttcagatat ccgtatgccg ggaatggacg ggctggcgct
    22321 gctcaagcag attaaacagc gccatccaat gcttccggtc atcattatga ccgcacattc
    22381 cgatctggat gctgccgtca gcgcctatca acaaggggcg tttgattatc tgcccaaacc
    22441 gtttgatatc gacgaagcag tggcgctggt tgagcgcgct atcagtcatt accaggaaca
    22501 gcagcagccg cgtaatgttc agcttaacgg cccaacgacc gatatcatcg gcgaagcgcc
    22561 agccatgcag gacgtgttcc gtattatcgg tcggctttcg cgttcttcta ttagcgtgct
    22621 gattaacggc gaatccggca ccggtaaaga actggtcgct catgccctgc atcgccacag
    22681 tccgcgcgcc aaagcgccgt ttatcgcgct gaatatggca gctatcccaa aagatttgat
    22741 cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact ggcgcgaata ccattcgtca
    22801 ggggcgtttt gaacaggccg atggcggtac attattcctc gacgaaattg gtgatatgcc
    22861 gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac ggtcagtttt accgcgttgg
    22921 cggctatgcg ccggtgaaag tggatgtgcg gattatcgct gccactcacc agaatctcga
    22981 acagcgagtg caggaaggta agttccgtga ggatctgttc caccgcctga acgttatccg
    23041 cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt ccccgtctgg cgcgccattt
    23101 tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag ttgctgcatc cggaaaccga
    23161 agctgctctg acgcgtctgg cgtggccagg caacgtacgc cagctggaaa acacctgccg
    23221 ctggctaacg gtgatggccg ccgggcagga agtgttgatt caggatttgc ccggcgaact
    23281 gtttgaatca acggttgcgg agagtacttc gcaaatgcaa ccggacagtt gggcgacact
    23341 tttagcgcag tgggcagaca gagcgctgcg ttccggtcat caaaatctgc tttccgaagc
    23401 gcagccagag ctggagcgga cgttactgac gaccgcgttg cgacatacgc aggggcataa
    23461 acaggaagcg gcgcggctac tcggctgggg ccgcaacacc ctgacgcgta agttaaaaga
    23521 gctggggatg gagtgattca cagcttgtgt gtaaagattg attattgagc gcaaattgct
    23581 ggtattttac gctttactgt tccgataagt tcagtatgat cttgcccgga aaacggggag
    23641 agtcattatg ctggaatcaa taattaatct ggtatcgagt ggcgcagttg acagccacac
    23701 accgcaaact gccgttgctg ccgtgctgtg tgccgcaatg attgggctgt ttagttgagt
    23761 ttcagattgc tgatgaagtg ctctttgttc atgccggatg cggcgtgaac gccttatccg
    23821 gcctacaaaa gcaatatttt caacaggttt tgtaggcctg ataagcgtag cgcatcaggc
    23881 aatcttgttc tcagcacatt gatggcgctt tcgttttcta ctttgtaaac gaagcgccat
    23941 tcactattta aatcacccgt gagaactgct gcatccgcgc tttctggcgc agataggtat
    24001 caaagcacat gcaaatgttg cggatcagca agcgaccttt cgccgtcacc tgtattccct
    24061 tctcatccac atccaccagc ccatcttttg ctaacggggc gagcagcttg agatcttccg
    24121 caaagtaatc agcgaagtgc aaatcccact gtttctcaat aggggcgtaa tccagacgga
    24181 agttgcagat gagcgactta atcacatcgc ggcgaataca gtcatcacgc gttagcgcaa
    24241 taccacgcca cagcgcattg ccttgttcat ccacttgctg atagtactgc ttcaactctt
    24301 tctggttctg cgcgtagcag tcgccaatca tgctgatggc ggaaacgccc atccccagca
    24361 gatcggtatc gccctgagtg gtgtagccct ggaagttacg atgcagcacg ccttcacgct
    24421 gggccaccgc cagctcgtca tccggacggg caaagtgatc cataccgata aactgatagc
    24481 ccgattgcgt caggaaggcg atggtttcct gcaggatatc gagtttttgc tgcggactcg
    24541 gcaggtcagc atctttgatt ttgcgctgag cagcaaaaat ggtcggcaga tgcgcgtagt
    24601 taaagacact cagacgatcg gggttcagct ccgccacacg tttcagggta aaggcgaaac
    24661 tctccggcgt ctgtttcggc aggccgtaaa tcaggtcgat gttggtggag gtaaaaccaa
    24721 tctcacgcgc atggttaagc agtgcaaaga tgaactcttc atcctgctcg cggttaacca
    24781 gacgttgcac ttctttgttg aagtcctgca cgcccatgct caggcgatta aagccttcgg
    24841 cgcgtaaatg atcgagtaca tccagttcga tttcccgcgg atcgacttcg atcgaaatct
    24901 ccgcatcggc attgaactgg aagttttcgc gcagcagctt catcaggcgg ctgatttgcg
    24961 ctttattcag atacgtcggc gttccgccgc cccagtgcaa ttggctgacg tgacgcccgg
    25021 cgaacagcgg tgcacgatgg acgatttctt gctccagcgc gtccagatac tgatcggcct
    25081 tgtgctgctg gcgagtaaca atcttattgc aaccgcagaa gtaacaaagc ttatggcaga
    25141 acgggatatg tacgtagaga gataatggac gctcaggata gcgcgccacg gcttgtaaaa
    25201 acgcctgttc gccgaagtct tctgaaaact ccagcgcggt cgggtacgag gtgtatcgtg
    25261 gcccggaata gttatatttc tggatcaggg ccagatccca gtcgatttgc tgtacagaca
    25321 tgctcactcc ttccgatggc gtctctggcg gctacggcgt accggcggat gcccgtttcg
    25381 ggtcatcagc cggttgcgca gccacttttg tcgccgcgac aaccgctgta gtttaacgaa
    25441 taaccacacc agataacata taaccggaag ggttatcagc agaacgggaa ggcccacggc
    25501 ggcaatccgt tagttgcccc gcagcagacg catcatgtct tcttgcttct cgtcttcttc
    25561 ctcttcttcg tcatcgtcat aagagaggcc gagtttctgc atcaactcat caatacgatc
    25621 cagtttagca tccacccagg attgctcttc ggcactcagg gtttcgcctg cttccagacg
    25681 ttccagcagc gcatccagac gctcatccgt ttccagtaat tccaactccg cctgcggtga
    25741 aagcataggt ttctcactct tcggtttgtg ctgtttggtg actttttcag tcacgcccaa
    25801 tggaataggg gttttactgc caatacgtgg atcttttggt gcgttctggc ctttgctgcc
    25861 tgacgtggtg tttccgcccg ctgcgcggct gcccggcgca tgaccacgac gttttttctg
    25921 acgcttgcga tcacgagctt cctgatccag ctcctcgcgt gtttttcgac gcgcttttgc
    25981 atgacctttg ctgcgtgagt ttgaagatga tggtttcata atggttcgtc ttttagccgt
    26041 tttattcagt atagatttgc ggcggaatct aacagaaagc aagcaaagaa aaaaggcgac
    26101 agattactct gtcgcctttt ttcctgactc ataaccctta acgggtctta caatccttgc
    26161 aggccgccaa cacaccctgt ttttccttta gccggaacaa tccgtgttga ttccatttcc
    26221 gtttaattac gtctccggac gtttgtcttc ctgacaatcc tctgtcttcg cctcctggcg
    26281 ctcctgaccc tcatcctgag tcattgttcc tgttagcgtc ctcgctctat gggtgctact
    26341 ttacgccttt gctttaaaca aacaatcaac cgccatcagt atttgcacat tttcagagtt
    26401 ttatataaaa gcaattcact gatttatata gcttatttga catgcatcgc agaattttct
    26461 ctgctatttc gcataaagac tatcggcaat gtctcacaaa gcgtatggct tttacttaca
    26521 acgctcacgc cgaaaaatcc gccttttatt atggttcagg tataatcgcc acaaagcttt
    26581 gactgacgga gacgaccgct ttgactaatt tgaattatca acagacgcat tttgtgatga
    26641 gtgcgcctga tattcgccac ctaccttccg ataccggaat tgaagtggct tttgcaggcc
    26701 gttccaacgc aggtaaatcc agcgcgctga acacgctgac taaccagaaa agcctggctc
    26761 gtacctcaaa aaccccaggg cgcacccagc ttatcaacct gtttgaagtg gctgacggca
    26821 agcgtctggt tgacttgcct gggtacggtt atgcggaagt cccggaagag atgaagcgca
    26881 aatggcagcg tgcgctcggc gaatacctcg aaaaacgtca gagcctgcaa ggtctggtgg
    26941 tgctaatgga tattcgccat ccgctgaaag atttggatca gcagatgatt gagtgggcgg
    27001 tagacagcaa tatcgccgtt ctggtgctgc tgaccaaagc ggacaaactg gcaagcggcg
    27061 cacgtaaagc gcaattgaat atggtgcgtg aagctgtact ggcgtttaac ggtgatgtgc
    27121 aggttgaaac gttttcttcg ttgaagaaac aaggcgtgga caagctgcgg cagaaactgg
    27181 atacctggtt tagcgagatg cagcctgtag aagaaacgca ggacggcgaa taattttctt
    27241 gccttaatgc ttgtgccgga tgtggcgtat ccggcccgta aattcaatag attacaattc
    27301 taccgttggc ctgataaaca tagcgcatca ggcattacgg atcttttctt tcgcccaata
    27361 aaaaacgccc cagtcattac tgactggggc ggctaaaata ttcagccaaa tccgattacg
    27421 tgaagtaaaa ggtctgaaag atagaacatc ttacctctgt accctacgcg actaacttta
    27481 ctcttttttg ttcagttcag aaagcacttt ttgtaatttt ttttcagttg ttgcatagga
    27541 atttctaata gccatcacaa aacgtgacag cttatgttgc ttacttacga aaaaagggca
    27601 tgttcaggcg aatcttagtg cgcctgatcc cagttttcgc cactccccac ttccaccagc
    27661 aacggcacat ccagacgggt acagttttcc atcagttgat gaatctgctt cgcgacggca
    27721 tcaacatcat ctttatgaac ttcaaatacc agttcatcgt gtacctgcat gatcatacgt
    27781 acacgcggtt gctcagcctg taaccacgca tcaacggcaa tcatcgcccg tttgataatg
    27841 tcggcggcgg ttccctgcat tggcgcgtta atggctgcac gttcagccgc tgcacgacga
    27901 gcaccattgc tggatttgat atccggcaga tacagacggc gtccgtccag cgtttcaacg
    27961 tagccctgct ctttcgcctg agcacgggtg cgttccatat actccagcac gccagggtag
    28021 cgttcgaagt aaaggtccat gtacttctgc gcttctttac gtggaatgtt caattgccgc
    28081 gccagaccga aagcactcat gccataaatc agaccaaagt tgatcgcttt cgcgctacgg
    28141 cgttgctcgc tggtgacggt ttccagtggc aaaccaaaca cttctgccgc cgttgcccgg
    28201 tggatatctt ttccttccgc gaatgcggtc agcaagcctt tgtcacgcga aagatgcgcc
    28261 ataatgcgca gttcaatctg cgagtagtcc gctgagacaa tcacataatc ctctggcgca
    28321 ataaacgcct ggcggatacg acgaccttct tcgttacgca ccggaatgtt ttgcaggtta
    28381 ggatcggttg acgataaacg tcccgttgca gttactgcct ggtgataaga ggtatgcaca
    28441 cgcccggttt tcgggttgat catcagcggc agcttgtcgg tgtaggtcga tttcagcttc
    28501 gccagaccac gatactccag aatcactttt ggcaacggat agtccagcgc cagttcttcc
    28561 agtacctctt ccgacgttga cggcgcgcca cccggcgttt tcttcagcgg tttaataccc
    28621 tgtttttcaa agagaatggt ttgcaactgc ttggtggaag aaaggttaaa ttcttcacct
    28681 gcaatttcat gcgctttctt ttccagctca gccagacgca aggtgagctc ttcagaatga
    28741 ttgtgcagca ctttcggatc gatcttcaca ccgttacgtt caatgcgtga aagcaccggc
    28801 accagcggca tttcgatatt ctcgaagacg ttcaacggcc ctttgtgttt ttgcagatct
    28861 ggccacattt tcagatgcaa ctgcaaggtg acatcagcat cttcggcggc gtaacgtccg
    28921 gcctcttcga gggcaatctg gttaaaggtc agttgatttt tgcctttacc cgcaatctct
    28981 tcaaaagtga tggttttgtg cttcaaccaa cgttccgcga ggctgtccat atcgtgacgc
    29041 ccggcaacgc tattgagaat gtaggactcc agcatggtat caaacgcaat cccacgcagc
    29101 tcaatgccgt aattcgccag aataccgcga tcgtatttca ggttttgccc gaccttcagc
    29161 gccttttcat cttccagcag cggttttagc aactcgagtg cacgctcgcg agagatttga
    29221 tcgggcgcgt caagataatc atgagcaacc ggaatatatg ccgctacgcc tggctcgata
    29281 gcaaaagaaa gcccgaccag gttagcagag atgttatcaa ggctgtcggt ttcggtatca
    29341 aatgcaaata ccggcgcttt ttccagcttc gcaatccacg ctttcagtgt ttcttcatca
    29401 aggatggtga cgtagttgtc ataagaaatc accgttgccg tcacttctgg cgcttcgtct
    29461 gcaacactgg tttcctgtgg cttcgcggct ggttttgccc ctttggcctg taaccatttg
    29521 cccgcttcga catcagcagt ccagcgtttg aactcatact ttttgaacag ccccaacaac
    29581 tcttccgctg ccggttgctg cacttccagt tgttcacagg tcagctccag ttcaacgtcg
    29641 gttttaatcg tcgccagctg gtatgagaga taagcaactt ctttgttttg ctcgagcttc
    29701 gctgccattg ttttcgcgcc acggaagctc aacccagcaa ttttttctgg ctcggcatac
    29761 agcgtatcca gtccgccaag accttgcagc aatgcctgcg cggttttttc accgacgccc
    29821 ggtacgccag gaatgttatc agaggagtca cccatcagcg ccaggaaatc gatgatcagt
    29881 tctggcggca cgccgtactt attcaccacc tcttccggtc cgaggatggt attcgtcatg
    29941 gtattgataa gcgtaatatt tggcgtcacc agctgcgcca tatctttatc gccagtgctg
    30001 atcagcaccg gacgcccggc tttttcggct tcgcgcgcca gagtaccgat aacgtcgtcc
    30061 gcttctacgc cagaaaccgc cagcagcggc agtcccatcg ctttaaccat cgcgtgcaag
    30121 ggttcgattt gtgcacgcag atcgtccggc attggcgggc gatgtgattt gtaatgttca
    30181 aacagttcat cacgaaaggt ttttcccttg gcgtcaaaga ccaccgctgc atgcgtcggt
    30241 ttatattgca tgatcagact gcgcagcatg ttgaggacac catacatcgc accggtcggc
    30301 tcgcctgcgc tgttagtcag cgggggaaac gcgtgatatg cgcgataaag ataagatgaa
    30361 ccatctacaa ggataagtgg attttggggg atctgaacca taatgtccgt gcctgtttat
    30421 cagattatgg gtaaaggatg ccacagacag gatgaaaaca tgagtttttc accgcttttc
    30481 gtgaaaatct tttgtagatc ttctggatcg ctcgcaaatc gtcatgtgga taactttgtg
    30541 cataaattat ttacatcgct tttaatttat tatcatgctt tttacagcat gatgtttgtc
    30601 tttgtttttc atgcagttaa aaataaagct atccctaata attgagataa tgggattttt
    30661 tctaatgtgg atagatatga attatttttc tccttaagga tcatccgtta tttgggtcgt
    30721 tttctgctaa aatctgcgcc ttgcgcttct ttagcgcact gtctatggct aaccttttga
    30781 ataaattcat tatgacgaga atactcgctg cgataaccct tttgctgagt atcgtattga
    30841 ccattttggt cactattttt tgttccgtcc cgatcatcat tgccgggatt gtaaaactat
    30901 tgctacctgt gccagtcatt tggcgaaagg tttcgcgttt ttgtgatttt atgatgtatt
    30961 gctggtgtga aggtctggcg gtattactgc atctgaaccc acacctgcaa tgggaagttc
    31021 acgggctgga agggttaagt aagaagaact ggtatctgct tatttgtaac caccgtagtt
    31081 gggcagatat tgtcgtactg tgcgtgctgt ttcgtaagca cattccgatg aataaatatt
    31141 tcctcaagca acagctggcc tgggtgccat ttcttggcct ggcgtgctgg tcgctggata
    31201 tgccgtttat gaagcgttat tcccgcgctt atttattacg tcatccggag cgacgtggta
    31261 aagatgttga aaccactcgg cgttcttgtg aaaagtttcg cctgcatccc accaccattg
    31321 tcaatttcgt tgaaggctcc cgtttcacgc aagaaaaaca tcagcaaacc cactccactt
    31381 ttcaaaactt gttgccacca aaggcggcag gcattgcgat ggcgctaaat gtactgggta
    31441 aacaattcga taaactgctt aatgttacgc tgtgttatcc ggacaataac cgtcagccgt
    31501 tcttcgatat gttaagcggt aaactgacgc gaattgtcgt tcatgtggat ttacagccta
    31561 ttgccgatga gttacatggc gactacatca atgacaaaag ctttaagcgc cattttcagc
    31621 aatggctgaa ctcactgtgg caggaaaaag accgacttct gacgtcgttg atgtcatcac
    31681 agcggcagga caagtaaatt ttgctcgcct ctgaatgagc agaggcgagt gagtatttta
    31741 atgaacaaaa cgtccggcac gagacataaa ttcttcttcg ctcatctcct gtccgttaaa
    31801 aacaacttta cccggtgtat aacgcagttg cagcgaggcg gtattgttgt caatcgtgat
    31861 taactgaaac atctgaccta aggtcatcat cccgctgatt tgtttatcag cccgcttttg
    31921 cgctttttcc gcatccattc cttcagataa attaagctgt tttgcggttt ctgtgacgac
    31981 attaagcggt aacttcacat caaaattgag cgatttaata tctttgtttg tggatgatga
    32041 agatttggct gggtcggcaa tactgatatc cagattggca tttagttcgc cgagtgcgtt
    32101 cttccatttt accggttgtt taatggtcgg ctcacttttt tgtaataacg gtaaatactc
    32161 tttgaacaat gcagcattca cttcttgcag agcaacttca tcgtttgcta actcagggtg
    32221 tgcagcaagc tgcttctgca ttgcaatgtt atactggatg ataaattggc gcaccgctga
    32281 gggatcaata gattcagcaa tcaatgacca ctcaccacta ccgagttgct gattcccttt
    32341 tgttaactta tcaagttcgt aggtcagatt gacattgata taatctttgt ctttaacgcg
    32401 atccagcatg gtttttgcaa cgaaagcatc gatttgggca acgtcttccc cgtgattgat
    32461 aatgttaatc tgattaaatt tcgactcgct ttcaccaacc ggaaatttct tttcttctaa
    32521 tcgcgccaga gaatcaatgg taaaactttt tgccgttact ttgttcattg gtgataattg
    32581 cagaacaata tcttcgactt tgccttcaat atccaggtta gacaatgaac taccatcacc
    32641 attaaaagta aactggccct caccaaaaga aaactttcca tactctgcag gggcaacatc
    32701 taatgtcgta gagaatgagc caccaaacgc gaatcgcaat gtcgcttcca cgggcgattt
    32761 atttttcgcg gcgataaata gcggttgtgt cagttcatta ttcactaagt tcacttttgc
    32821 cgctgccagt gctgggatga cattaccatg cattaacatt gtgatgggca acggaccgtg
    32881 ctcaacatcc acatcaaata caactttctg gcctggcttg atattgagat cgggtgcacc
    32941 gttatcgaat gtcatttgca tttgaaaacg agaattaaaa aaacctttgt caaaattttt
    33001 ataacttaac gtcatatcgt aggcatgttc acctttcttt ttagcatcgt taaaatcttt
    33061 aataaacttt tcgacaccag gctgaatttg cgtacctgtg taccatgtgc caccacccca
    33121 gattacggct aacgcaacaa taacacctgt agctgactta cgtatcatat aaatattccc
    33181 tgataaaaca gcaaaacttt aaatatccgc ttatgctgaa ataatgtcca gc
//
LOCUS       DAPHDX010000040        32432 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPHDX010000040 DAPHDX010000000
VERSION     DAPHDX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.040"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(65..1825)
                     /locus_tag="RH058_001857"
     CDS             complement(65..1825)
                     /locus_tag="RH058_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDV3137449.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1955..2647
                     /locus_tag="RH058_001858"
     CDS             1955..2647
                     /locus_tag="RH058_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV3137450.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2846..3934
                     /gene="serC"
                     /locus_tag="RH058_001859"
     CDS             2846..3934
                     /gene="serC"
                     /locus_tag="RH058_001859"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV3137451.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4005..5288
                     /gene="aroA"
                     /locus_tag="RH058_001860"
     CDS             4005..5288
                     /gene="aroA"
                     /locus_tag="RH058_001860"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV3137452.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5457..6221
                     /gene="ycaL"
                     /locus_tag="RH058_001861"
     CDS             5457..6221
                     /gene="ycaL"
                     /locus_tag="RH058_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV3137453.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6394..7077
                     /gene="cmk"
                     /locus_tag="RH058_001862"
     CDS             6394..7077
                     /gene="cmk"
                     /locus_tag="RH058_001862"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV3137454.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7188..8861
                     /gene="rpsA"
                     /locus_tag="RH058_001863"
     CDS             7188..8861
                     /gene="rpsA"
                     /locus_tag="RH058_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV3137455.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9021..9305
                     /gene="ihfB"
                     /locus_tag="RH058_001864"
     CDS             9021..9305
                     /gene="ihfB"
                     /locus_tag="RH058_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV3137456.1"
                     /translation="MTKSELIERLATQQSHISAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9512..11776
                     /locus_tag="RH058_001865"
     CDS             9512..11776
                     /locus_tag="RH058_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV3137457.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11813..13561
                     /gene="msbA"
                     /locus_tag="RH058_001866"
     CDS             11813..13561
                     /gene="msbA"
                     /locus_tag="RH058_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV3137458.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13558..14544
                     /gene="lpxK"
                     /locus_tag="RH058_001867"
     CDS             13558..14544
                     /gene="lpxK"
                     /locus_tag="RH058_001867"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV3137459.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14581..15813
                     /locus_tag="RH058_001868"
     CDS             14581..15813
                     /locus_tag="RH058_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV3137460.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15865..16047
                     /gene="ycaR"
                     /locus_tag="RH058_001869"
     CDS             15865..16047
                     /gene="ycaR"
                     /locus_tag="RH058_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV3137461.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16044..16790
                     /gene="kdsB"
                     /locus_tag="RH058_001870"
     CDS             16044..16790
                     /gene="kdsB"
                     /locus_tag="RH058_001870"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV3137462.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16944..17837
                     /locus_tag="RH058_001871"
     CDS             16944..17837
                     /locus_tag="RH058_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV3137463.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="RH058_001872"
     CDS             complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="RH058_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV3137464.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18729..19514
                     /gene="cmoM"
                     /locus_tag="RH058_001873"
     CDS             18729..19514
                     /gene="cmoM"
                     /locus_tag="RH058_001873"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV3137465.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19511..20833
                     /gene="mukF"
                     /locus_tag="RH058_001874"
     CDS             19511..20833
                     /gene="mukF"
                     /locus_tag="RH058_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV3137466.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20814..21518
                     /gene="mukE"
                     /locus_tag="RH058_001875"
     CDS             20814..21518
                     /gene="mukE"
                     /locus_tag="RH058_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV3137467.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21518..25978
                     /gene="mukB"
                     /locus_tag="RH058_001876"
     CDS             21518..25978
                     /gene="mukB"
                     /locus_tag="RH058_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV3137468.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLCERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26239..28086
                     /gene="ldtD"
                     /locus_tag="RH058_001877"
     CDS             26239..28086
                     /gene="ldtD"
                     /locus_tag="RH058_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3137469.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28267..28815
                     /locus_tag="RH058_001878"
     CDS             28267..28815
                     /locus_tag="RH058_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV3137470.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSPGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28842..29489
                     /gene="gloC"
                     /locus_tag="RH058_001879"
     CDS             28842..29489
                     /gene="gloC"
                     /locus_tag="RH058_001879"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV3137471.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29711..30901)
                     /gene="aspC"
                     /locus_tag="RH058_001880"
     CDS             complement(29711..30901)
                     /gene="aspC"
                     /locus_tag="RH058_001880"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV3137472.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31086..32174)
                     /gene="ompF"
                     /locus_tag="RH058_001881"
     CDS             complement(31086..32174)
                     /gene="ompF"
                     /locus_tag="RH058_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV3137473.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7902 a         7881 c         8715 g         7934 t
ORIGIN      
        1 aaaaaacgcc ccgtaatata actcagacta atcattaagc ctacattgcg taggctattt
       61 gattttattt tgcccagaat gctgctttgg cgcgctgcag cttttcgtag gccttcaaca
      121 acgactgatg tgcagcaaac gcgtgcagat cgctatctac cggttgcagg ccgtaaaacg
      181 ccgcttcgcc gctcattgcc gcactggcgg cttctacggc atctgcgccg tacatgcgaa
      241 caaacgcatt cagatattgc agcggctggc gatcttcttc ctgtgccagt aataacaacg
      301 tttgcaggca gcgataatag ttggcgcgtt ccggactaaa tactgatgag ttaaactcca
      361 tcgtccattc ggtccagacc agagcctgtt ccagatcgcc accagccagc gccagcatgg
      421 cttttaattc accgatacgc agggtgtacc aaccgttatc cgacccggtc gccagaccca
      481 acagctcacg cacgcgggta aagtcatcaa aaccttcttc atccagttgc tcgatgaggt
      541 tcaggtaatc ttctttttcc cactcgctgc ctggtagcga aagaatcgtt tcacgtaaat
      601 ggctgcccat actgttattc gcgagccaca gatcttcagc cggataaata tcggacatgc
      661 caggcacgat aatgcggcaa gcgtagacac ccagatgctc gtaatcggca atataaactt
      721 ctttatcttc ctttttgaag atagccatca gcgtggcgaa ctcttcttcc gtggtgccgg
      781 agaaattcca gtccacaaac ggataatcgg catcctgctt gaacaggtcc caggagatta
      841 aaccgctgga atcgataaag tgcgtttcga ggttggtatg ttcagcgact tcttcatcat
      901 cgaaggttgg cggagtaaac acatccaaat ctttcaggcc acgaccttgc agcagctcgg
      961 tcacggtacg ttccagtgct acgccaaaat caggatgcgc accgaaagag gcaaagcagg
     1021 taccgttagc aggattgaac agtaccacgc aaatcaccgg atactggccg ccaagcgaac
     1081 cgtcataagc gaagattggg aaaccctccg cttccagcgt ttcgatggct tcaactactg
     1141 ctgggtaacg cgccagcacg tctgccggga tctctggcag gctgatgctt tcagcaataa
     1201 tgcggttttt cacgtagcgt tcgaaaactt cagacaatcc ctgaacgcgt gcttcgttgc
     1261 gggtattacc tgcggacata ccgttggaga cgtacaggtt accaatgata ttcatcggaa
     1321 tataaacggt ctgattgtcg gactgacgcg taaacggcag gccgcaaata ccacgatctt
     1381 cgttaccgga ttgtaggtca atcagcatgc tgccggtcag ttcattctcc ggatcataaa
     1441 acgcgcgcag acggtcatcg agtagtcctt ctggcacatc gtcattttcg gtcagtggga
     1501 accatttttc gttgggataa tgcacgaacg gaccgttggc gatggtttcg cccagccaga
     1561 aatcagcaaa aaagtagttg gttgagagac gctcgaaata ttcaccaagt gcagaagcca
     1621 gcgccgcttt tttggttgcg ccttttccgt tggtaaaaca cagtgcgcac tctttgtcgc
     1681 gaatatgtac agaccagacg ttaggcacag gattcagcca ggaggcctct tcaatctgaa
     1741 agccgaggtc tgaaagtttt tgctggaagc gagcgatgga atcttccaga gcggcatctt
     1801 tgccggggat aaatgtttgc gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata
     1861 atacgggttt tatctcaaag gcgctatcac cgccgccatg ccgatgaact gttgactatg
     1921 cttttagcgg ataaaccacg ataagagcat aaaaatgaag gcgttcgatc tccaccgtat
     1981 ggcatttgat aaagtgcctt ttgatttcct tggcgaagtt gcactacgta gtctttatac
     2041 ctttgtactg gtttttttgt tcctcaaaat gaccggaaga cgcggtgtgc gacagatgtc
     2101 gctgtttgaa gtgttaatca ttctcacgct gggttcagcg gcgggagatg tggcgtttta
     2161 tgatgatgtg ccgatggtgc cggtacttat cgtctttatc actctggcgt tgttataccg
     2221 cctggtaatg tggttgatgg cgcacagtga aaaactggaa gatctactgg aaggtaagcc
     2281 ggttgttatt attgaagatg gcgagctggc ctggtcgaaa ctcaataact ccaacatgac
     2341 ggaatttgag ttctttatgg agctacgatt gcgtggcgtg gagcagctgg ggcaggtacg
     2401 tctggcgatt ctcgaaacca acgggcaaat cagtgtctat ttctttgaag atgacaaggt
     2461 gaaaccgggt ttacttattt tacccagtga ttgtacgcag cgttacaaag tggtgccgga
     2521 gtcggcggac tatgcctgta ttcgttgtag tgaaatcatt catatgaaag cgggggaaaa
     2581 acaattatgt ccgcgctgtg caaatccaga atggacgaag gcaagtcggg caaaacgggt
     2641 gacctgacag taaaaacatc ggctttttgc taataatccg agagattctt ttgtgtgatg
     2701 caagccacat ttttgccctc aacggtttta ctcattgcga tgtgtgtcac tgaatgataa
     2761 aaccgatagc cacaggaata atgtattacc tgtggtcgca atcgattgac cgcgggttaa
     2821 tagcaacgca acgtggtgag gggaaatggc tcaaatcttc aattttagtt ctggtccggc
     2881 aatgctaccg gcagaggtgc ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct
     2941 tggtacgtcg gtgatggaag tgagtcaccg tggcaaagag ttcattcagg ttgcagagga
     3001 agccgagaag gattttcgcg atcttcttaa tgtcccctcc aactacaagg tattattctg
     3061 ccatggcggt ggtcgcggtc agtttgctgc ggtaccgctg aatattctcg gtgataaaac
     3121 caccgcagat tatgttgatg ccggttactg ggcggcaagt gccattaaag aagcgaaaaa
     3181 atactgcacg cctaatgtct ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa
     3241 gccaatgcgt gaatggcaac tctctgataa tgctgcttat atgcattact gcccgaatga
     3301 aaccatcgac ggtatcgcca tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc
     3361 cgccgacttc tcttcaacca ttctttcccg tccgattgac gtcagccgtt atggcgtgat
     3421 ttacgctggc gcgcagaaaa atatcggccc ggctggcctg acaatcgtca tcgttcgtga
     3481 agatttgctg ggcaaagcga atatcgcgtg tccgtcgatt ctggattatt ccatcctcaa
     3541 cgataacggc tccatgttta acacgccgcc gacatttgcc tggtatctat ctggtctggt
     3601 ctttaaatgg ctgaaagcga acggcggtgt agctgaaatg gataaaatca atcagcaaaa
     3661 agcagaactg ctatatgggg tgattgataa cagcgatttc taccgcaatg acgtggcgaa
     3721 agctaaccgt tcgcggatga acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt
     3781 gttccttgaa gagtcttttg ctgctggcct tcatgcactg aaaggtcacc gtgtggtcgg
     3841 cggaatgcgc gcttctattt ataacgccat gccgctggaa ggcgttaaag cgctgacaga
     3901 cttcatggtt gagttcgaac gccgtcacgg ttaatgccga aattttgctt aatccccaca
     3961 gccagcctgt ggggttttta tttctgttgt agagagttga gttcatggaa tccctgacgt
     4021 tacaacccat cgctcgtgtc gatggcacta ttaatctgcc cggttccaag agcgtttcta
     4081 accgcgcttt attgctggcg gcattagcac acggcaaaac agtattaacc aatctgctgg
     4141 atagcgatga cgtgcgccat atgctgaatg cattaacagc gttaggggta agctatacgc
     4201 tttcagccga tcgtacgcgt tgcgaaatta tcggtaacgg cggtccatta cacgcagaag
     4261 gtgccctgga gttgttcctc ggtaacgccg gaacggcaat gcgtccgctg gcggcagctc
     4321 tttgtctggg tagcaatgat attgtgctga ccggtgagcc gcgtatgaaa gaacgcccga
     4381 ttggtcatct ggtggatgcg ctgcgcctgg gcggggcgaa gatcacttac ctggaacaag
     4441 aaaattatcc gccgttgcgt ttacagggcg gctttactgg cggcaacgtt gacgttgatg
     4501 gctccgtttc cagccaattc ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag
     4561 atacggtgat tcgtattaaa ggcgatctgg tttctaaacc ttatatcgac atcacactca
     4621 atctgatgaa gacgtttggt gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa
     4681 aaggcgggca gtcttatcag tctccgggta cttatttggt cgaaggcgat gcatcttcgg
     4741 cttcttactt tctggcagca gcagcaatca aaggcggcac tgtaaaagtg accggtattg
     4801 gacgtaacag tatgcagggt gatattcgct ttgctgatgt gctggaaaaa atgggcgcga
     4861 ccatttgctg gggcgatgat tatatttcct gcacgcgtgg tgaactgaac gctattgata
     4921 tggatatgaa ccatattcct gatgcggcga tgaccattgc cacggcggcg ttatttgcaa
     4981 aaggcaccac cacgctgcgc aatatctata actggcgtgt taaagagacc gatcgcctgt
     5041 ttgcgatggc aacagaactg cgtaaagtcg gcgcggaagt ggaagagggg cacgattaca
     5101 ttcgtatcac tcctccggaa aaactgaact ttgccgagat cgcgacatac aatgatcacc
     5161 ggatggcgat gtgtttctcg ctggtggcgt tgtcagatac accagtgacg attcttgatc
     5221 ccaaatgcac ggccaaaaca tttccggatt atttcgagca gttggcgcgg attagccagg
     5281 cagcctgaat gaacaacggg caataaatag ccaaatcttt ctttatcaaa acgtcggcac
     5341 attgtcggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
     5401 caatgtaagt tgttgataca taatatttat atatgattaa tcaacggatg attcacatga
     5461 agaatactaa attactgctg gcgattgcga cctctgcagc attactgaca gggtgtcaaa
     5521 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
     5581 caacattaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
     5641 gcggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
     5701 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
     5761 acgtcaacgc atgggcgatg gcgaacggct gtgtccgtgt ctacagtggc ctgatggaca
     5821 tgatgaatga caacgaaatt gaaggtgttc tgggccatga actgggccac gtcgcgttgg
     5881 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
     5941 cagctaccag cggtgtggct tcccagcttt cccgctcaca attgggtgat atcgcagaag
     6001 gcgctattaa tgctaaatac tcccgtgata aagagtccga agcagatgat ttctcctttg
     6061 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
     6121 ctagtctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
     6181 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
     6241 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
     6301 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     6361 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     6421 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcattg
     6481 caatggcatc tgctggattc gggtgcaatt tatcgcgtac tggcattggc ggcattacat
     6541 caccatgttg atgttgcgtc agaagatgcg ctggtaccgc tggcatccca tctggatgta
     6601 cgttttgtgt caaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     6661 gaaattcgta ctcaggaagt ggcgaatgca gcttcacaag tcgcggcatt cccacgcgtt
     6721 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat
     6781 ggccgcgaca tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc
     6841 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     6901 aactttgagc gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     6961 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     7021 gagcaagtga ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg
     7081 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     7141 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     7201 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     7261 gtggcgttgt tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg
     7321 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     7381 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     7441 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     7501 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     7561 acggtattcg tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc
     7621 tgcacctgga aggcaaagag cttgaattta aagtaatcaa gctggatcag aagcgcaaca
     7681 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     7741 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     7801 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct
     7861 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     7921 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ttgggcgaag
     7981 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     8041 ccaacctgac cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac
     8101 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     8161 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg
     8221 gtctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg
     8281 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     8341 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     8401 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     8461 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     8521 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     8581 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     8641 gtgaccgcgt tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     8701 tcaccggcgt tgatcgtaaa aaccgcgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     8761 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     8821 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     8881 ttttattccg aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     8941 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     9001 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     9061 aatcgcacat ttccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     9121 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     9181 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag
     9241 gaaaatacgt tcctcacttt aaacctggta aagaactgcg cgatcgcgcc aatatttacg
     9301 gttaagtttt tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcattt
     9361 ctctaatcag gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     9421 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     9481 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     9541 aatttgcgga atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc
     9601 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     9661 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgtgggc
     9721 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg caactgacgg
     9781 catgaccact cattacggtc aaattactca tctacaaggt cgacgtatat tccctgcgcc
     9841 aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgctggac aacaatggtc
     9901 aatgaaactc aaagttcgtg cggttcatgg ccaacttaat gatggcggct ttgatagcca
     9961 gcgttatgcc attgcccagc atcaaccgct caccggccgc tttctgcagg caagtgttat
    10021 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
    10081 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccccaa
    10141 agaaatcaaa aatatcatgc gcgatactgg aacggcgcat ttaatggcga tatcgggatt
    10201 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
    10261 gcctgggcgc tggatccact ggcaaatacc attaattggt gggatctgct gtgctgcttt
    10321 ttatgcctgg ttgacgggaa tgcaacctcc tgcattgcgt accgtggtgg cgcttgctac
    10381 gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
    10441 tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt tatggctctc
    10501 tgccgctgcg gtcgcggcac tgattttttg gtatcagtgg tttccctgtc ctgagtggca
    10561 actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg gtatcacact
    10621 tctgcttatg cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct cgtttattgc
    10681 aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat
    10741 ggttgtgcat ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc ttgccgaccg
    10801 gtctttggct ttacttttct gggggttaaa gagtttgcca gaagggtgga tcaacattgc
    10861 tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa
    10921 cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct
    10981 atggcaaaaa cctcgacctg acgagtggca ggtgtacatg cttgatgtcg ggcaagggct
    11041 ggcaatggtg atagccagaa acggcaaagc gattctctat gacacaggac tggcctggcc
    11101 cgaaggggat agtgggcaac aactgattat cccctggctc cactggcata atcttgaacc
    11161 ggaaggcgtt attctgagtc atgaacatct ggatcaccgg ggagggctgg actcaatatt
    11221 gcacacatgg ccgatgttat ggatcagaag tccgttaaac tgggaacatc atcagccctg
    11281 tgtgcgtggc gaagcgtggc aatggcaagg attgcgtttc agcgtgcact ggcctttaca
    11341 agctagcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg acgggacgaa
    11401 tagcattctt ctaaccggtg atattgaagt ccccgctgaa caaaagatgc taagccgtta
    11461 ctggcagcaa gtgcagacaa cattgcttca ggtacctcac catggcagta atacctcatc
    11521 atcgttgcca ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta
    11581 taacgcatgg cgattgccct ctaataaagt taagcatcgc tatcaacagc aaggatatca
    11641 atggcttgat actccacatc agggtcaagt gacggtcaat ttttcagcgc aaggctggcg
    11701 gattagcagc ctcagagagc aaattttacc tcgttggtat catcagtggt ttggcgtgcc
    11761 agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt gaatgcataa
    11821 cgacaaagat ctctctacgt ggcagacgtt ccgccgactg tggccaacca ttgcgccttt
    11881 taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt
    11941 catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt
    12001 gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta
    12061 tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg
    12121 cctgtttggt cacatgatgg gaatgccggt ttcatttttt gacaaacagt caacggggac
    12181 gctgttgtca cgtatcacct acgattccga acaggttgct tcttcttctt ccggcgcact
    12241 gataacggtt gtgcgtgaag gtgcgtcaat catcggcctg ttcatcatga tgttctatta
    12301 cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg
    12361 cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt
    12421 gaccaccagc gccgaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca
    12481 ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa
    12541 aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct
    12601 ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac
    12661 gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cactgaccaa
    12721 cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga
    12781 cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga
    12841 attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa
    12901 cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt caggtaaatc
    12961 aaccatcgcc agcctgatca ctcgttttta cgatattgat gaaggcgaaa tcctgatgga
    13021 tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc
    13081 gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga
    13141 acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat
    13201 caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg
    13261 cggtcagcgt cagcgtattg ctatcgctcg agccttgctg cgtgatagcc cgattctgat
    13321 tctggatgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact
    13381 ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga
    13441 aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa
    13501 cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg
    13561 atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc actctcctgg
    13621 ttgtatggcc tggtgagtgg cgcgatccgt ctttgctata aactaaaact gaagcgcgcc
    13681 tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa
    13741 accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggaattcg agtgggggtc
    13801 gtatcgcggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc ggcagatacc
    13861 acaacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactga tgcgcctgtt
    13921 gcggtttctc ccgttcgttc tgatgcggta aaagccattc tggcgcaaca ccctgatgtg
    13981 cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga tgtggaaatt
    14041 gtcgttattg atggtgtgcg tcgctttggc aatggctggt ggttgccggc ggggccaatg
    14101 cgtgagcgag cggggcgctt aaagtcagtt gatgcggtaa tcgtcaacgg cggtgtcccc
    14161 cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt acgtaccggt
    14221 acgcgttgtg atgttgctca gcttgaacat gtggtggcga tggcagggat tgggcatccg
    14281 ccgcgctttt ttgccacgct gaagatgtgc ggcgtacaac cggaaaaatg tgtaccgctg
    14341 gccgatcatc agtctttgaa ccatgcggat gtcagcgcgt tggtaagcac cgggcaaacg
    14401 ctggtaatga ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga aaattggtgg
    14461 tatttgcctg tagacgcaca gctttcaggt gatgaaccag cgaaactgct tacgcaacta
    14521 acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat ggagtcatga
    14581 atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc cgcacaaggc
    14641 ctgttaaaca aaccccgccg tcgagcgtcg ttggaggata ttccggcaac gatctcccgc
    14701 atgtccttgc tgcaaatcga taccatcaat attgttgccc gtagtccata tctggtgctt
    14761 ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc gcgtggcgaa
    14821 ttaatggaat actgggcgca tgaagcctgc tttatgccac gcagcgactt tcgtcttatt
    14881 cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag
    14941 gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg gccggtacgt
    15001 tcagccgatt ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat
    15061 aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga acggcgcaac
    15121 ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga tgagcgcgat
    15181 ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg tagcctggga
    15241 atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc actggcggcg
    15301 tggcgcgaag cgagggctga acagcagcaa atcattgctg tgcatgttga aaaattgggc
    15361 aatctttggc tgcatgctga tttgctgccg ctactcgagc gtgcgctggc cggaaagctc
    15421 actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga tcgcaaacgc
    15481 gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc gccgaaacgc
    15541 cagtatggct attttgttct gccattatta catcgtgggc aattagttgg gcgaatggat
    15601 gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt acaggaaggc
    15661 attaaaccaa cgacaatgct gcaaaaaggg ttacgtcagg cgattactga tttcgcgagc
    15721 tggcagcagg caacgcgggt gacattagga cgctgcccgc aaggtctctt tactgattgc
    15781 cgcactggct gggaaataga ccccgtcgca taaatgatta tgataagatt tatgcattca
    15841 taacccgatc catctggagg aactatggat catcgtctgc ttgaaatcat tgcctgcccg
    15901 gtttgcaacg gaaaactttg gtataaccaa gaaaaacaag aacttatttg caaactggac
    15961 aacctggctt tcccgctgcg tgatggcatt ccagtgttgc tggaaacgga agcccgcgtg
    16021 ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt
    16081 caacgcgtct gcccggtaaa ccattggttg atattaatgg caaacccatg attgttcatg
    16141 ttcttgaacg tgctcgcgaa tcaggtgccg atcgcatcat cgtggcaacc gatcatgagg
    16201 atgtcgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc gccgatcacc
    16261 agtcagggac agaacgcctg gcggaagttg tcgaaaaatg cgcattcagc gacgacacgg
    16321 tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt cgtcaggttg
    16381 ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca atccacaatg
    16441 cggaagaagc gtttaacccg aatgcggtga aagtggttct cgacgctgaa gggtatgcac
    16501 tgtacttctc tcgcgccacc attccttggg atcgtgatcg ttttgcagaa ggccttgaaa
    16561 ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc
    16621 gtcgttacgt caactggcag ccaagtccgt tagaacacat cgaaatgtta gagcagcttc
    16681 gtgttctgtg gtacggcgaa aaaatccatg ttgctgttgc tcaggaagtt cctggcacag
    16741 gtgtggatac ccctgaagat cttgagcgcg ttcgcgctga aatgcgctaa tttcacttca
    16801 cgacacttca gccaattttg ggaggagtgt cgtaccgtta cgattttcct caatttttct
    16861 tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt atgaaagcag
    16921 tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc catttactgg
    16981 gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt
    17041 tgtatgacag ccagggaaac ccaatgccgt taatggcaag aagctttagt acgcccggaa
    17101 aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact gtccgtatgc
    17161 cgactattta tggtgtgatg acgcatgaag aacaccccgg ccccgatgtc ctgctactgg
    17221 agcggatgcg tggtgtttcg gtggaggcac cagcccgaac accagaacgc tgggaacaac
    17281 tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt cgcggttgcg
    17341 tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg caacatgttg
    17401 aagtgctatg gaccacgctc aatcagttca ataacaccgg tctgacgatg caggataagc
    17461 ggatcctgtt tcgcactcgc gaatgtctcc cggcattatt tgaaggcttt aacgacaatt
    17521 gtgtgttgat tcacggtaac ttctgtttac gcagcatgct gaaagattcg cgcagcgatc
    17581 agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa tacgaactgt
    17641 tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg caacgcgcgc
    17701 cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc
    17761 aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca aaatcactct
    17821 tgccgtggct cgcctgacgg acctttcagc cactgccaga tgcggccaag cgtttcatag
    17881 ccaacacgat cgctatgcat cagccagacc ggagaaggga ttgcccgttc ccacggatta
    17941 agcggcgagt cgatggccaa ctgattcgcc ggggctggta gcggatttaa cccttcctgc
    18001 tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag gaaaggggcg
    18061 tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg caaatccagg
    18121 gtgataattt gctcgcgcgg cacgcccagc gattgcgcaa ctctggcacc tacttccgct
    18181 gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga tcccggattt
    18241 tcgcgccata agcgaatacc ttcattcagg cgtggcagac tgttattgat taaattagag
    18301 ctcggtgccc actgcgggtt ccaggtataa ccacctccga gcaccacgat atagtccact
    18361 ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct
    18421 accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa aatcttcccg
    18481 gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat taacaacatc
    18541 agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct
    18601 ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat
    18661 tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg
    18721 agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc cgtaacattt
    18781 acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc
    18841 tggcggaaat gggcccgcaa aaactgcgtg tgctggatgc tggcggtgga gaagggcaga
    18901 ccgcaatcaa aatggccgag cgtgggcatc aggtcatttt atgcgatctt tctgcgcaga
    18961 tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta
    19021 tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt
    19081 tccatgcggt gctcgagtgg gtggccgatc cccgcagtgt attgcagacc ctgtggtcag
    19141 tgttgcgtcc aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc
    19201 ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac
    19261 ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag
    19321 ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga
    19381 agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc
    19441 aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca
    19501 aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa
    19561 aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg cggttgccac
    19621 gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg
    19681 ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaataacgc
    19741 gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcggaagg
    19801 gaacgcaatt taccgtctga cgccgctcgg catcggcatc actgactatt acattcgtca
    19861 gcgcgagttt tctacgctgc gcctttctat gcagttgtcg attgtggcgg gtgagctcaa
    19921 acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgttta
    19981 tgctccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct
    20041 gatggatgaa cagcagcagc aggtgaagga tgatatcgcc cagttgctga acaaagactg
    20101 gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga
    20161 attgcaggat acgctggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca
    20221 ggatgcgacg atgacccatg acgatctgca tttcgtcgat cgtctggtgt tcgatctgca
    20281 gagcaaactc gatcgtatta tcagttgggg ccagcaatcc atcgacttgt ggattggcta
    20341 cgaccgccac gtacacaaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt
    20401 tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaactta
    20461 tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga
    20521 agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga tccgcgaaca
    20581 gctggcggcg atcatcgaag aacaacttgc cgtgtacaaa accagacaag tgccgctgga
    20641 tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt
    20701 tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg
    20761 actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat
    20821 cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat
    20881 ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac gaactggata
    20941 atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg
    21001 ttgagcttat tcgcgcacca gaagggttct tctatttacg cccacgttcc accacgctga
    21061 tccctcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgttatctct
    21121 atctcagccc ggaacggctg gcgaatgagg ggattttcac ccagcaggaa ctgtacgacg
    21181 aactgctcac cctggccgat gaagcaaaac tgctgaaact ggtgaacaac cgttcaaccg
    21241 gttcagacgt tgaccgtcag aagttgcagg agaaagtacg ttcttcgctc aaccgtctgc
    21301 gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagtttcgc attaccgaat
    21361 cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc
    21421 gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa
    21481 ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg
    21541 ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc ttgacgagct
    21601 ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg cggcgttcgt
    21661 tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc
    21721 caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc
    21781 gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca
    21841 ggttgccgga cgcgatcgta aagtggatat caagccgttt gccattcagg gactgccgat
    21901 gtcggtgcag ccgacacagc tggtgaccga aaccctgaac gaacgccagg cgcgcgtgct
    21961 gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt
    22021 caactccatt actgattacc actcgctgat gttcgatctg ggcatcatcg cgcgtcgtct
    22081 gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg
    22141 gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt
    22201 gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgtatga cgctggaagc
    22261 gattcgtgtc acccagtcgg accgcgacct gtttaagcat ctgatcagcg aagccaccaa
    22321 ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc
    22381 cctggagttt cgtcgcgagc tacatacttc gcgtcagcaa ctggcggctg agcagtacaa
    22441 acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc
    22501 ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga
    22561 gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg aagagcaaaa
    22621 tgaagtggtg gcagaagcca tcgaacgcca ggaagagaat gaggctcgtg cggaagctgc
    22681 cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt
    22741 ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc gtgccaaaga
    22801 actgtgccat ctgccggact taaccgccga ctgcgccgcc gaatggctgg aaaccttcca
    22861 ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc
    22921 gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc
    22981 gctggcgcgt aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag gggtcgatca
    23041 gcgtcacctg gcagagcagg ttcaaccgtt gcggatgcgc ttaagtgaac tggaacagcg
    23101 tctgcgcgag cagcaagaag ccgaacgcct gctggcagac ttctgcaaac gtcagggcaa
    23161 gaattttgat attgatgaac tggaagctct gcatcaggag ctggaagcgc gcattgcctc
    23221 tctttccgat agcgtgtcta acgcccgtga agagcgcatg gcactgcgcc aggaacagga
    23281 acagctgcag tctcgcattc agagtttgat gcagcgtgcg ccggtttggc tggcagcgca
    23341 aaacagtctc aaccagttga gcgagcagtg cggtgaagag tttacctcca gccaggacgt
    23401 caccgaatat ctgcaacagt tgctggagcg tgagcgagag gcgattgttg aacgcgatga
    23461 agtgggcgcg cgcaaaaacg ctgtcgatga agagatcgaa cgtttaagcc agcctggcgg
    23521 ctctgaagat cagcgtctga acgcgctggc ggagcgtttt ggtggtgtgc tgctgtcaga
    23581 aatttatgac gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc
    23641 acgccacgcc atcgtggtgc cagatctgtc acaggtaact gaacacctgg aaggcttgac
    23701 cgattgcccg gaagatctct atctgatcga aggagatccg cagtcattcg atgacagcgt
    23761 gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg
    23821 ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga
    23881 aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgatgt
    23941 acagaaaact cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc atctggcggt
    24001 tgcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact
    24061 ggagcgggcg ttaagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca
    24121 ggcgaaagag ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga
    24181 tgacagtctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc
    24241 cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga ttgtttcggt
    24301 attgcagagc gacccggaac agttcgaaca gttaaaagaa gattacgcgt actctcagca
    24361 gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc
    24421 gcactttagc tattctgact cggcagaaat gcttagcggt aacagcgatc tcaacgaaaa
    24481 actgcgtgaa cgtctggaac aggcggaagc ggagcgtacc cgcgctcgcg aagcgttgcg
    24541 cggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttctta
    24601 cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg
    24661 tgctgatagc ggggcagaag agcgggcgcg tattcgccgt gacgagctgc atgcgcaact
    24721 gagcaataac cgttcacgcc gcaatcaact ggaaaaagcg cttaccttct gcgaagcgga
    24781 gatggacaac ctgacccgca aactgcgcaa gctggagcgg gattactttg agatgcgcga
    24841 gcaggtagtg accgccaaag cgggctggtg tgcggtgatg cgcatggtga aagataacgg
    24901 cgttgagcgc cgcttacacc gtcgtgagct ggcttatctc tccgctgatg atttgcgttc
    24961 catgtcggat aaggcgttag gtgcgctgcg tctggcggtg gcggataacg aacatctgcg
    25021 cgacgtgctg cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc agttcttcgt
    25081 ggcggtttat cagcatctgt gtgaacgtat tcgtcaggat attattcgta ccgatgatcc
    25141 ggtggaagct atcgaacaga tggagattga acttagccgt ctgaccgaag aattaacctc
    25201 ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat
    25261 tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg
    25321 tcaggtgaac agcgtgcgtc tcaacgtgaa cgtgcgtgaa acgcacgcca tgctactgga
    25381 tgtgctctct gaacagcacg agcagcatca ggatctgttt aacagcaacc gtttgacctt
    25441 ctcggaagcg ctggcgaaac tgtatcaacg tcttaacccg cagattgata tggggcagcg
    25501 cacgccgcag accatcggtg aagaactgct ggattaccgc aactatctgg aaatggaagt
    25561 tgaggttaac cgtggttccg atggctggct gcgcgcagag tctggtgcat tgtcgaccgg
    25621 tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga
    25681 atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc
    25741 agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgaac gtttgcaaat
    25801 gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact
    25861 ggtgcgtaaa gtcttccaga ataccgaaca cgttcacgtc gtcggcctgc gaggatttgc
    25921 gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa
    25981 ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa
    26041 gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta
    26101 tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg
    26161 tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat
    26221 aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc
    26281 agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa
    26341 gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg
    26401 caagccacgg caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc
    26461 cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt
    26521 caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa
    26581 gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa
    26641 tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct
    26701 gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg
    26761 ctatatagca gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg
    26821 cagctggcgc tggataaagg tcaattgcct acgtttgttg ctggactggc accgcagcat
    26881 ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg
    26941 ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg
    27001 ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct
    27061 ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca
    27121 gaaactaagc cgatggacaa gcaaacgaca tctcgtagta aacctgcgcc tgccgttcgc
    27181 gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg
    27241 ggggcagatg gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag
    27301 cgtgctggcg tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct
    27361 accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag
    27421 gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt
    27481 gcccttaaca acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa
    27541 gatattctgc caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg
    27601 atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc
    27661 acggcctcga atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg
    27721 cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac
    27781 aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct
    27841 tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat
    27901 gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc
    27961 tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac
    28021 aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc
    28081 aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga
    28141 ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg
    28201 ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg
    28261 attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca
    28321 ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc
    28381 attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc
    28441 agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg
    28501 aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg
    28561 ttaggcacgc gcaaaccggt gcaactcatt tccggttatc gttctattga taccaacaat
    28621 gaactacgcg cccgcagccc tggagtagcg aagaaaagct atcacactaa aggccaggcg
    28681 atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg
    28741 cgcgcaggtg gtgtaggata ttatccacgt agtaactttg tgcatattga taccgggcca
    28801 gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat cgtattattc
    28861 cggtcaccgc attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg
    28921 cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc
    28981 tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg
    29041 aactggcgca acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc
    29101 tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc
    29161 cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt
    29221 tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc
    29281 tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg
    29341 atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga
    29401 catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct
    29461 tcctgcaaga cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg
    29521 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
    29581 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
    29641 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
    29701 tttttaatgc ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg
    29761 tcatcccggc cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca
    29821 gacgcagcac ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga
    29881 taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca
    29941 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat
    30001 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag
    30061 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa
    30121 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
    30181 caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca
    30241 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaactg
    30301 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
    30361 agcagccatg gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg
    30421 catcgaagtc aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac
    30481 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct
    30541 taacgctggt attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg
    30601 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
    30661 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
    30721 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
    30781 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
    30841 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
    30901 tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta
    30961 caaaattcca actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
    31021 tggaaggtat aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggta
    31081 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
    31141 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
    31201 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
    31261 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
    31321 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
    31381 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
    31441 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
    31501 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
    31561 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
    31621 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
    31681 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
    31741 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
    31801 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
    31861 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
    31921 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
    31981 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
    32041 aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag
    32101 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
    32161 ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt
    32221 caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt
    32281 gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat
    32341 aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt
    32401 gaacaccgtc atatttccat agaaacgtga tg
//
LOCUS       DAPHDX010000041        32328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPHDX010000041 DAPHDX010000000
VERSION     DAPHDX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.041"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(404..498)
                     /locus_tag="RH058_001882"
     tRNA            complement(404..498)
                     /locus_tag="RH058_001882"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(462..464),aa:Sec,seq:tca)
     gene            791..2173
                     /locus_tag="RH058_001883"
     CDS             791..2173
                     /locus_tag="RH058_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV3137474.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2183..4501
                     /gene="yicI"
                     /locus_tag="RH058_001884"
     CDS             2183..4501
                     /gene="yicI"
                     /locus_tag="RH058_001884"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV3137475.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLSNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4554..6263)
                     /locus_tag="RH058_001885"
     CDS             complement(4554..6263)
                     /locus_tag="RH058_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV3137476.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(6384..7775)
                     /gene="xanP"
                     /locus_tag="RH058_001886"
     CDS             complement(6384..7775)
                     /gene="xanP"
                     /locus_tag="RH058_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV3137477.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8055..9260
                     /gene="gltS"
                     /locus_tag="RH058_001887"
     CDS             8055..9260
                     /gene="gltS"
                     /locus_tag="RH058_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV3137478.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            9263..10129
                     /locus_tag="RH058_001888"
     CDS             9263..10129
                     /locus_tag="RH058_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137479.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISERDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(10114..12195)
                     /gene="recG"
                     /locus_tag="RH058_001889"
     CDS             complement(10114..12195)
                     /gene="recG"
                     /locus_tag="RH058_001889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV3137480.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPEMERYSNA"
     gene            complement(12201..12890)
                     /gene="trmH"
                     /locus_tag="RH058_001890"
     CDS             complement(12201..12890)
                     /gene="trmH"
                     /locus_tag="RH058_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV3137481.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12897..15005)
                     /gene="spoT"
                     /locus_tag="RH058_001891"
     CDS             complement(12897..15005)
                     /gene="spoT"
                     /locus_tag="RH058_001891"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV3137482.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15024..15299)
                     /gene="rpoZ"
                     /locus_tag="RH058_001892"
     CDS             complement(15024..15299)
                     /gene="rpoZ"
                     /locus_tag="RH058_001892"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV3137483.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15354..15977)
                     /gene="gmk"
                     /locus_tag="RH058_001893"
     CDS             complement(15354..15977)
                     /gene="gmk"
                     /locus_tag="RH058_001893"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV3137484.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16235..17917
                     /gene="ligB"
                     /locus_tag="RH058_001894"
     CDS             16235..17917
                     /gene="ligB"
                     /locus_tag="RH058_001894"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV3137485.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWEEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17914..18531)
                     /locus_tag="RH058_001895"
     CDS             complement(17914..18531)
                     /locus_tag="RH058_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV3137486.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18823..19647)
                     /gene="dinD"
                     /locus_tag="RH058_001896"
     CDS             complement(18823..19647)
                     /gene="dinD"
                     /locus_tag="RH058_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDV3137487.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19868..20731)
                     /locus_tag="RH058_001897"
     CDS             complement(19868..20731)
                     /locus_tag="RH058_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV3137488.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20858..21574
                     /gene="rph"
                     /locus_tag="RH058_001898"
     CDS             20858..21574
                     /gene="rph"
                     /locus_tag="RH058_001898"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV3137489.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21640..22281
                     /gene="pyrE"
                     /locus_tag="RH058_001899"
     CDS             21640..22281
                     /gene="pyrE"
                     /locus_tag="RH058_001899"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV3137490.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22318..22914)
                     /gene="slmA"
                     /locus_tag="RH058_001900"
     CDS             complement(22318..22914)
                     /gene="slmA"
                     /locus_tag="RH058_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV3137491.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23021..23479)
                     /gene="dut"
                     /locus_tag="RH058_001901"
     CDS             complement(23021..23479)
                     /gene="dut"
                     /locus_tag="RH058_001901"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV3137492.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRDQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23457..24677)
                     /gene="coaBC"
                     /locus_tag="RH058_001902"
     CDS             complement(23457..24677)
                     /gene="coaBC"
                     /locus_tag="RH058_001902"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV3137493.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24849..25517
                     /gene="radC"
                     /locus_tag="RH058_001903"
     CDS             24849..25517
                     /gene="radC"
                     /locus_tag="RH058_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV3137494.1"
                     /translation="MKNNSQLMMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25734..25970
                     /gene="rpmB"
                     /locus_tag="RH058_001904"
     CDS             25734..25970
                     /gene="rpmB"
                     /locus_tag="RH058_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV3137495.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25991..26158
                     /gene="rpmG"
                     /locus_tag="RH058_001905"
     CDS             25991..26158
                     /gene="rpmG"
                     /locus_tag="RH058_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV3137496.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26256..27065
                     /gene="mutM"
                     /locus_tag="RH058_001906"
     CDS             26256..27065
                     /gene="mutM"
                     /locus_tag="RH058_001906"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV3137497.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27104..27583)
                     /gene="coaD"
                     /locus_tag="RH058_001907"
     CDS             complement(27104..27583)
                     /gene="coaD"
                     /locus_tag="RH058_001907"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV3137498.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27591..28868)
                     /gene="waaA"
                     /locus_tag="RH058_001908"
     CDS             complement(27591..28868)
                     /gene="waaA"
                     /locus_tag="RH058_001908"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV3137499.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29281..30339
                     /gene="rfaQ"
                     /locus_tag="RH058_001909"
     CDS             29281..30339
                     /gene="rfaQ"
                     /locus_tag="RH058_001909"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV3137500.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30336..31460
                     /locus_tag="RH058_001910"
     CDS             30336..31460
                     /locus_tag="RH058_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV3137501.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31453..32250
                     /gene="rfaP"
                     /locus_tag="RH058_001911"
     CDS             31453..32250
                     /gene="rfaP"
                     /locus_tag="RH058_001911"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV3137502.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7645 a         8015 c         8678 g         7990 t
ORIGIN      
        1 aagttgattt gatatattaa atgaacaaat taatcttgat ggcagtctga ttattggtga
       61 agtttatagt cagttttttt cgccagcggt taaagaaata gaatagtcgt ataaaaaagg
      121 gtgttctggg gcgagtaaaa caacatcttc accttaatgg cgatacagtt ggctgagagt
      181 cagcaggggg gcaaatcacc tgcaaataaa cacggcgcat actggcgtga atatcgcgga
      241 tgcgttcgag gacagaagaa ccgacaggcg gattcttcag ccgctcatcg tccatcacaa
      301 aaccttagac caggtattcc tgaagccgat tgaatattga gcagtagaaa atatctggat
      361 tgacagtatt acaaggggtt ggagaacgtc aggagaggca ttttggcgga agatcacagg
      421 agtcgaacct gcccgggacc gctggcggcc ccaactggat ttgaagtcca gccgcctcac
      481 cggagacgac gatcttccgc gcctcgattg ctacatggag gcggggcgca ttatagctac
      541 ttccttgagt ttctacatcc cccagatcag ttttttccgc ctaatacacg cagcgtatta
      601 tcacgaaagc attatagttc atataaaacc catattttac aatgatatat gagcggaatt
      661 aacgctacca cgatcacata aaacaagaat cataaattaa taaccagata tcggaatatt
      721 cgctctcaca gggatggttt acaaaatgcg ttatcggtct cagcacccct atagcatcaa
      781 ggaaaagcag atgaagagtg aagtgttgtc cgttaaagag aaaattggtt atggcatggg
      841 agacgccgcc agccacatta ttttcgataa cgtaatgtta tatatgatgt tcttttatac
      901 cgatattttt ggcattcctg ccggatttgt cggaaccatg tttttggtcg ctcgtgcact
      961 ggatgcgatt tccgatcctt gcatggggtt gttggccgat cgaacgcgct ctcgctgggg
     1021 taaatttcgt ccgtgggtac tgtttggcgc actgccattc gggatcgtct gtgtactggc
     1081 ctatagcacg ccagatctca gtatgaacgg caaaatgatc tatgcagcaa ttacttacac
     1141 cctacttacc ttactttata ccgtcgtcaa tatcccttac tgcgcattgg gtggtgtaat
     1201 caccaatgac ccgactcagc gtatctcgct gcaatcctgg cgttttgtgc tggcgaccgc
     1261 gggaggcatg ctttctactg ttctgatgat gccactggtt aatttaattg gcggtgataa
     1321 taaaccactc ggtttccagg gcggtatcgc ggtcctttcc gtggtggcat tcatgatgct
     1381 ggcattttgt ttcttcacca ctaaagaacg cgttgaagca ccacctacaa caacgtctat
     1441 gcgggaagat ttacgtgata tctggcaaaa cgaccagtgg cggattgtcg gtttactaac
     1501 cattttcaat atcctggcgg tgtgcgtacg cggtggggcg atgatgtatt acgtcacatg
     1561 gattttgggc acgccggaag tgtttgtcgc ttttctcacc acttattgcg tgggtaacct
     1621 gattggttcc gcactggcaa aacctctgac cgactggaaa tgtaaagtca ctatcttctg
     1681 gtggacgaac gccctgctgg cagtgattag cctcgcgatg ttctttgttc ccatgcaggc
     1741 cagcatcact atgtttgtct tcatcttcgt gattggtgtg ttgcatcaac tggtgacacc
     1801 tatccagtgg gtaatgatgt ccgataccgt cgactacggc gagtggtgca atggtaaacg
     1861 cctgaccggg atcagttttg ctggcacgct gtttgtgctc aaactggggt tggccttcgg
     1921 cggcgctctt atcggctgga tgctggctta tggcggatat gatgcggcag aaaaagcgca
     1981 gaacagcgcc acgattagca tcattattgc gctattcacg attgttccgg cgatctgtta
     2041 tttgctgagc gcgattatcg ctaaacgcta ctactcactc acgacgcaca atctgaaaac
     2101 cgttatggaa cagctggctc agggtaaacg ccgttgccag caacaattca cctctcaaga
     2161 agtgcagaac taaggaacgg caatgaaaat tagcgatgga aactggttga ttcaacctgg
     2221 cctcaatttg attcacccgc ttcaggtgtt cgaggttgaa cagcaggata atgaaatggt
     2281 ggtctatgct gccccccgtg atgtgcgtga acgtacctgg cagcttgata cgcctttatt
     2341 tacgttgcgc tttttctccc cacaggaagg tattgtcggt gtgcggattg agcattttca
     2401 gggggcgctg aataacggtc ctcattatcc gctcaatatt ttgcaggacg tgaaggtcac
     2461 aatcgaaaac acagaacgtt atgctgagtt taaaagtggc aacttaagcg cgcgtgtcag
     2521 caaaggtgag ttctggtcac tggattttct gagcaacggc gaacgtatta ccggtagtca
     2581 ggtgaaaaat aatggctacg tgcaggacac gaataatcaa cgcaattata tgtttgagcg
     2641 gcttgatctt ggcgttggcg aaacagttta cggtctggga gagcgcttta ctgccctggt
     2701 gcgcaatggc cagacggtag agacctggaa ccgggacggc ggcacaagta ctgaacaggc
     2761 gtataaaaat atcccgttct acatgactaa ccgtggttat ggggtactgg tcaatcatcc
     2821 ccagtgtgtc tcttttgaag tgggatcgga gaaagtctcc aaagtgcagt tcagcgttga
     2881 gagtgaatat ctcgaatact ttgttatcga cggcccgacg ccgaaagcgg tacttgatcg
     2941 ttatacccgc tttactggtc gtccggcgct gccgcccgcg tggtccttcg gcctgtggct
     3001 aaccacttca tttaccacca actacgacga agcgacggta aacagcttta tcgatggtat
     3061 ggcggaacgc aatctgccgc tgcatgtttt ccactttgac tgtttctgga tgaaagcctt
     3121 ccagtggtgc gattttgagt gggacccgct gactttccct gacccggaag ggatgatccg
     3181 ccgcctgaaa gcgaaaggac tgaaaatctg cgtctggatt aacccctata tcggtcaaaa
     3241 atcccccgtc tttaaagagt tacaagagaa aggctattta ctcaaacgcc cggacggttc
     3301 gctatggcag tgggataaat ggcagccagg tctggcgatt tatgacttta ccaatccgga
     3361 tgcctgcaaa tggtacgccg acaaactgaa aggtctggtc gcgatgggcg ttgattgctt
     3421 taagaccgac tttggcgaac gtatcccaac tgatgttcag tggtttgacg gttccgatcc
     3481 gcagaaaatg cataaccatt atgcgtacat ctacaacgaa ctggtgtgga acgtgctcaa
     3541 ggacaccgtt ggtgaggaag aagctgtctt gtttgcccgc tcggcctccg tcggtgcgca
     3601 gaaattcccg gtacactggg gtggcgattg ttacgctaac tacgaatcaa tggcggaaag
     3661 cctgcgcggt ggtttgtcta ttggcctttc aggttttggc ttctggagcc acgatatcgg
     3721 cggctttgaa aataccgctc cggcgcacgt ttacaaacgc tggtgcgcgt ttggtttgct
     3781 ctccagccat agccgtttac acggtagcaa atcttatcgt gtgccgtggg cctacgatga
     3841 tgagtcctgt gatgtggtgc gcttcttcac gcaactgaaa tgccgcatga tgccgtatct
     3901 gtatcgtgaa gctgcgcgtg cgaacgcgcg gggtacgccg atgatgcggg ccatgatgat
     3961 ggagttcccg gacgatccgg cttgtgatta ccttgaccgt caatacatgt taggcgacaa
     4021 cgtgatggtt gcgccggtgt tcactgaagc gggcgatgtg cagttctacc tgccggaagg
     4081 tcgctggaca cacctgtggc acaacgatga gctcgacggt agtcgctggc ataaacagca
     4141 gcacagcttc ctgagtctgc ccatttatgt gcgtgataac accctactgg cgctgggcaa
     4201 caacgatcaa cgtcccgatt acgtgtggca cgaaggcacg gcattccacc tctttaatct
     4261 gcaagacggg catgaagccg tctgtgaagt gcccgctact gacggatcgg tgatctttac
     4321 tttaaaagca gcacgtactg gcaacacgat tactgtgact ggtgcgggcg aggcgaagaa
     4381 ctggacgctg tgcctgcgca atgttgtgaa agtaaatggt ctgcaagacg gttcgcaggc
     4441 tgaaagtgag caggggctgg tggtgaagcc tcaagggaat gcgctgacaa ttacgttgta
     4501 atctattgat ggaatgccgg gtacgtctga gtcgtacccg gcgagattga tgtttaccgc
     4561 accggctccg tcgatgtaac ttcaccgctg gaaacgatgc cagcattcat ggtttgagtc
     4621 acttgctgct ttgcggcgtt cacggcatgc aattgcccgc taaccgtagg tttcaacggg
     4681 acattggcct gaatatcgcc actggcggta agctgaatat tgccgtcgcc agtcaacggt
     4741 aatttaggcc atccccactg ttgcaaaata ttcaccggca caccgcgtcc attcaggctg
     4801 atatgtgtct gacgttgtgg cgtttgtgaa acactggcgg tggcttccag aatgcctttt
     4861 tcagtaaatg cactcagttc gctgatattc accgtgctgc tgttggcggt cagcgccagc
     4921 gacgggcgac gaacatcaac acgattgaat gtcgcggcgg cggcattcag attcgccgag
     4981 ccactccaga cgccccattt atgatcggta accagcgtca ggttggcacc gtaaccatcg
     5041 agcgcggtga gctgccacgg gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg
     5101 ctaaatctct tcagttgcag gctgtttaac caaccgggtg tcgtttccat ccacaactgt
     5161 tgccagtttt tcggcaaggt atattccagc ccggcaattg ccgcatcatc aaggatcaac
     5221 gttttcccgt cacgcagcca gttccctgac gttctgacca tacccccttc ccagcggctg
     5281 gtgaactggc gcagcgctac gccctgcggg gaaaattccg cgtttataat cgggtcaaat
     5341 aaatgcagcg aaccataaat gaactcgcta gcgttcatcg acagtttgcc ttcttgtgtc
     5401 tgccagtcat ctttactgaa ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc
     5461 cagtccggac cttgcaaacg agcatcgatc acttccaggc gaccaatctg caacgacggg
     5521 acagagcgta atggcgcaaa gaagtcggtt agcgattttt cgctttgtag acggatatcc
     5581 gccatgcgca gattttccac ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt
     5641 aatgtcccgc gggcgatgtc ggcacccagg ttagtcagcg taacgcgatc gttatcaata
     5701 ctgccttcaa tcagtacatt ggtggcagga acatcattga gcgaaagcga tccggcacta
     5761 aactgaatct gcgccttcgt acccagcact ttaccggctt ctggtgacca cggaaccacg
     5821 ccgccattta cccgctgcgc gctcagtttc cattcgctat tcgggctatt aaacgccata
     5881 tcacgcagtt gcagacgatc ggctttgaac ggtagcggcg cggtctggtc ggtgagattc
     5941 agcgtcccgt tttccagcag gatggtatcg acatggcgtg gttcggtcag ttgccgactg
     6001 cttagcgcaa tgtcgacact ttttgccacc agggtcgcgg gctgaccatc acgaccaaac
     6061 gtgacgttct ccagcacgat atgagatggc gcggaaaaac ggtgatccat cgccccgaag
     6121 gccagatgat agtcgctatt ctcggaaacc catgcgctga tatgttctgc tccccagcgg
     6181 gtttgcagaa gaaaatagag gccagcgatc accactaaca gagcgatgag aatgtagaga
     6241 agcagcttcc caataaattt catggtcttc catcccgtga aatgcacata agggagttat
     6301 gcacgattta cgcgcaatcc tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta
     6361 attatcttac cgccgccagt gaattactgt ttttctggtg ggaaaatcag attcagcacg
     6421 atggcagtaa taccgcccgc ggcgatcccg gaggagagca ggtttttcag ccattcaggg
     6481 gcaaactgca aaatcagcgg ctgctgagac acgcccagac caaccgccag cgacagcgcg
     6541 ataatcagaa tcgcccgacg gttcagcggc tcacgagaaa cgatacgcac accggaggcg
     6601 gcgatggtgc caaacattac aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt
     6661 acaaaaccgc tcactgccgg gaacagaccc agcacgatca acatcagcgc gacgacaaaa
     6721 ccgacatagc ggctggcaac accagtcaac tggatcactc cgttgttttg cccgaagcag
     6781 gagttcggga aggtgttaaa caccgccgaa acaaacgagt tcaggccgtt tgccagcacg
     6841 ccgcctttca ggcgtttcat gtacagcgga ccggacactg gctgttcgga aacgtcagag
     6901 gtcgccgtga tatcgccaat ggtttccagc gaagtgatca taaagaccag catcagcggc
     6961 agcagcagac tccattcaat gccaagaccg taatagagcg gcgttggcac cataatcagt
     7021 tcttgcgtca tcggttcgtt gctttctggc aacatgccca taaaccacgc cagcgcatat
     7081 ccggccgcca tcgcaatcac cagtgaggcc acgcgtaagt aagggttacg ttgacggtta
     7141 agcaggataa ttaaggctaa gaccacgcct gccagcagca gatttttcgg tgcgccgaag
     7201 gtgttatcgc tcatggctgc gtaaccgccg ccaatggacg ttaacccaac ctgaattagc
     7261 gacaggccga taatcatcac cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc
     7321 agatgcagaa cgcgggagat caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac
     7381 aaagccgcca tcatggtagg aacatcagca ccaccggttt tcagcgcggt accgcccata
     7441 atcagcgggg caacaaagtt gaagctggtg ccctgaatag acaacagccc ggagccaacc
     7501 ggaccccagg ccttaatttg aataatcgat gccacaccgg aggcaaacag cgacatacta
     7561 ataatgtgtt gcgtgtcttg tgccggtaaa cccagcgcct ggcagattaa tagcgctggc
     7621 gtgatcaccg caacgaacat cgccagcaga tgctgacagg cggcaaacag ggtttgagga
     7681 agcggcggac gatcttcaag acggtaaatc agttcgctgt tttgagtctg cgcaaccggt
     7741 tgcgcatttt ctgactcgag ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag
     7801 cgggcatttt agctgaccga aagagaaaag caaacgtttg ccacatcctc atctaaatat
     7861 ttaaatgaga taaatgcact tttttatata acttttggta tttttctgct taaaatccat
     7921 gccatttgtt cattaacagc tgagaatttg acccaggaaa gcataactcg atacccgtca
     7981 tagttcatga agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat
     8041 acaaaggagt aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga
     8101 cgttgctgct cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac
     8161 cggaacctgt tgcgggtggt ttgttggtgg cgctggcgct actagtactg aaaaaaagca
     8221 tgggctggga agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg
     8281 ccaccattgg cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca
     8341 tcttcttgat tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg
     8401 ctagcctgtt agggcttgat ccgctgatgg ggctgttggc cggttctatt acgctttccg
     8461 gcggtcacgg tacgggcgct gcgtggagta aattgttcat tgaacgttat ggcttcacca
     8521 atgcgacgga agtggcgatg gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg
     8581 gcggtccggt ggcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg
     8641 accaggaagt cccgacggcg tttgaaaagc cggatgtggg acgcatgatc acctcgctgg
     8701 tgctgattga aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc
     8761 aacttttggc tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg
     8821 tgattctgag caacggtctg tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat
     8881 ccgtgctggg taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac
     8941 tgtgggagct ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct
     9001 tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg
     9061 cagtgctggc tgcgggtcac tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca
     9121 acatgcaggc gatcactgaa cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga
     9181 tggtcggtgc gttctttatc gatatcgtca atgcgctggt gattaagctg tatttaatgt
     9241 tgccgatttt tgccggttaa aaatgaaaaa gcgtcggaag gaaccggaaa cgctacgtga
     9301 acactgtcgc catatttttg gtgatgagcc gccagtgctc tgtgtatggg aaacggaatt
     9361 tgattacgca gatgccgaac tcaaggcgct ggcggctaaa gagtggcagc agatcagcga
     9421 acgggatttg agtgcttact atgtactcaa tctcgtttat aacgaaccga tgcaaataga
     9481 gttgtttcgc tatctgtttc cgctctgcct ggcgcaatgg catgaaaccg ttttagctgg
     9541 cggttatggt gatcacttcg aagaatcctt aatgaaggca ttgtgtcgac cctatctctg
     9601 gcaggagatg atgaatgctt cccaacgcca gcaggtacgg caatttttac tggacaccgc
     9661 gttgcagcgt atggataacg agcgtggttt caataacgtt ctttgctggc tggcggtgtt
     9721 caatactctg ggtggggccg cgccgttaat tcgttctctc tggtcgcgct ggtgggcgct
     9781 ggatacgcca ggaaaagcgg tgtgtgctat tcagtatgcc gcgcatctaa tttatcccat
     9841 agaagccaat ccgttatggt cgcaagaatg gataggctgg gggcatccat taggccataa
     9901 agatggctgg tcctctgata atcgcgcttt tcttcgccaa atgttaacac ctgaaatgat
     9961 tgtggctggc gtacaggcag cggctgaaat tctgcgtggt gaaccggaag gcgcaatggc
    10021 ggcgcgaatt gcacaggatg cgtatgaggc aatggatatc ctcactatcc agattgaaga
    10081 tttactgcgt gacttgtctt gtgatgagtc tggtcatgcg ttggagtaac gttccatctc
    10141 cggcatccag cgttctatca gggcttttgc ttgttgtggg taacgttcgt gaatatgtcg
    10201 cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa
    10261 ctcagcatta cccgtctgac gcgtacctaa cagttcaccc ggtccgcgaa tctctaagtc
    10321 tttctgtgca atcacaaaac cgtcgttgct gtcgcgcagc acttgcagcc gaatttgcgc
    10381 ggttttggag agcggcgttt tgtagagcag cacgcagtga gaagccaccg caccacgacc
    10441 tacacgcccg cgaagctggt gtaactgcgc cagacccaga cgctccgggt tttcgataat
    10501 catcagactg gcgttaggca catcaacgcc gacttcaata acggttgtgg caaccagcag
    10561 gtgtagctca ccttgtttaa acgacgccat caccgcctgt ttctcggcag gtttcatccg
    10621 cccgtgtacc aggccaacgt tcaactctgg tagcgccagt ttcaactctt cccaggtagc
    10681 ttccgccgcc tgcgcttcca gcaattccga ctcttcaatc aacgtacaaa cccagtacgc
    10741 ctgacgacct tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg tacggcgggt
    10801 atcaggaata gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga
    10861 ggtatcgaga tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat
    10921 gatcaactga tgcggatgga agccttgctg ctggcctttc tcccataacg ccagacgctg
    10981 atgcacgcca aagcgatgtt gttcgtcgat aatcaccagc gccagaccgt taaactgcac
    11041 ctgttcctgg aagatggcgt gcgtaccaac aatcatctgc acctgaccgc tggcgatggc
    11101 ttcctgctga gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc ccacttcgat
    11161 accgagcggt tcaaaccagt tgcggaagtt attggcgtgc tgttcggcga gtaattcggt
    11221 tggtgccatc aatgccacct gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc
    11281 gaccagcgtt ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag
    11341 cgccatatcg cgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg
    11401 taaggctgcg aggagtttat ttttcagcgc atcattggcg ctcagcggct gggcatgaaa
    11461 acgctgtgct ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag
    11521 aatcagacga cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg
    11581 tggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc
    11641 cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa
    11701 tttacgcagc gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc
    11761 ctgtaattct ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc
    11821 accatattta ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct
    11881 atttttcatt gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct
    11941 gatctggcag gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc
    12001 ttccaccgtg gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc
    12061 ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc
    12121 cagtttgtta ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa
    12181 caggcgacct ttcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca
    12241 gcttcgatct cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact
    12301 ttcgccagca ccggatagcc gccttcaaac aacaggcgtt gttgctctgc ttccggcaac
    12361 atgctgtttt cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga
    12421 atgagggctg aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg
    12481 atgtcctgat ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc
    12541 aaaatgcagg tcgggcgagt gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga
    12601 tgggttgcca gaatctgcat gccccggcct ttgagatgag cgacggcatc gccaatggtg
    12661 cggtgtgttt tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc
    12721 atgcggctac caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga
    12781 ataatcgcag aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc
    12841 tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa
    12901 tttcggtttc gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc
    12961 gccagatgca cacggtcacg ggcggtcaga cgaataaagg cgctgtagac gcgaccatct
    13021 ttctcttccg tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg
    13081 tttgccagcg cgccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc
    13141 gccgtctctt tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg
    13201 atgttacggc aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata
    13261 atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatcggca
    13321 cctttaatgg gcagatgtcc gtggctttgg gttgccggtg gaatggaggc gtccccatgt
    13381 tgcagatttt tcgcgaccac cacgctcatt gcgttaccaa gtccgatttc tgccagcaga
    13441 tcgtcaagcg ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt
    13501 tcattgagct tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca
    13561 gaatcatcac gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc ttttgagcta
    13621 acgacaaagt tcagccacgc ggcattcggg cgagcgcccg gtgcggtaat gatttcaacg
    13681 gtttgaccgc tggtaagcgg ctgcgacagc gggtaaggct ggcggtcaac gcgtgcgccc
    13741 acgcaggcat gaccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg
    13801 gcaggcagct cgacaattcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga
    13861 tcggatttaa cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc
    13921 aggctttgca tccagcgctg ggcgcggatt tgtgcggtag tactggtttc gccgtgctct
    13981 ttataagccc agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg
    14041 atctggacct caaccggcac gccgtgcggg ccaatcatcg aggtgtgcaa cgactgatag
    14101 ccgttcgctt taggaatggc gatatagtct ttcacgcgac ccggacgcgg cttgtacagg
    14161 ctatgcattt gcccgagcac gcgataacag gtgtcagaat cattgacgat cacgcggaaa
    14221 gcgtagatgt ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc
    14281 gaataaagat gcttttcgcg accactgacg cggcacggta ttcccgcttc ctgcaaacgc
    14341 ccttcgattt cagagaggat tttttggatc atctctttac ggttgccgcg cgcggctttc
    14401 accacttctt taattacgcg gtaacgattg ggatacagcg cctcaaaacc cagctcttcg
    14461 agttcggttt taatgtggtg gatacctaaa cggtgcgcca gcgggctata aatttcgaga
    14521 gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg
    14581 tgggtacggt cggcaagttt gatgagaatg acgcggatat cctgcaccat cgccataatc
    14641 atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc ggaacttgag cttatcaagt
    14701 ttcgacaccc cctctaccag ctcggcgacg cttttaccaa aaagctgttc catgtcctgg
    14761 taggtggcgg gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag
    14821 tcgagtttca tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca
    14881 ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga
    14941 cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga
    15001 tacaagggcg acccgctttg tgattaacga cgaccttcag caatagcggt aacggcttgt
    15061 aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg
    15121 ttgatcagac cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc
    15181 ggtaccagcg gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt
    15241 accaggtcaa aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata
    15301 cttaaaaagc tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct
    15361 gccaacaatt tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt
    15421 tcggcgcgaa taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc
    15481 agataatcat attcggcgta atggctcatt tctgcaacag cttgcgccat acgctttgca
    15541 atgacctctt cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttggac
    15601 ggcggtaaaa taaagatact ccgcgcgtgc ggcattttct ggcgaatttg ctgcgcgccc
    15661 tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca
    15721 cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta
    15781 atcatttctt taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca
    15841 ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt
    15901 tttaataaag cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa
    15961 agcgtgcctt gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg
    16021 agcttattat acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag
    16081 ttgttgcgtt ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa taaagacgat
    16141 tctcaggaag cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg
    16201 tatatctgcc agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat
    16261 aagtatcttg tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag
    16321 agcacaggaa gaaatttccc gcctgcaaca gcaaataaaa cagtgggacg atgactactg
    16381 gaaggaagga aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac
    16441 gcagtggcaa cgctgctttg ggagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg
    16501 tgcggttatg catccggttg cccatacggg tgtacgcaaa atggtggata aaaacgcatt
    16561 aagtctgtgg atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc
    16621 ggtaaccctg gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct
    16681 gaaaggcgag gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag
    16741 cgggccttta gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat
    16801 ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca
    16861 ggacgatagc gacacgctga attctctggg cgtttttgtc tgggcatggc cggatggacc
    16921 gcagttaatg accgatcgtt taaaggagct ggctaccgca ggttttactc taacgcagag
    16981 gtatacccgt gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa
    17041 agcgaaatta cccttcgtca ccgatggcgt ggttgtgcga ggggcgaaag aacccgaatc
    17101 ccgccattgg ctaccaggcc aggcagaatg gctggtggcc tggaaatatc aacctgtagc
    17161 tcaggttgcc gaagtgaagg cgattcagtt tgcggtgggt aagagcggta aaatatcggt
    17221 ggttgcgtca ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg
    17281 ttccgtcagg cgctgggagg agtgggatat tgcgcctggt gatcagatcc tcgtcagcct
    17341 tgccggtcag ggtattcctc gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa
    17401 accgacacca ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg
    17461 tcaggaacag ttcatttcac gcttagtctg gctggggtca aaacaggttc tggggctgga
    17521 tggcattggt gaggccggct ggcgcgcgct gcatcagact catcgctttg agcatatctt
    17581 ttcctggctt ttattaacgc cagagcaatt acagaacacg ccggggatcg cgaaaagtaa
    17641 aagtgcgcag ctatggcatc agtttaatct ggctcgtaag cagcctttta ctcgctgggt
    17701 gatggcaatg ggaataccgc taacccgggc ggcgcttaat gccagtgatg aacggtcctg
    17761 gtcgcaactt ttatttagca cggagcagtt ctggcagcag ctgccgggga ctggatcggg
    17821 gcgcgccaga caggttattg aatggaagga aaatgcgcaa atcaagaagc tgggcagttg
    17881 gctggctgcc cagcagatca caggttttga accttagtgt ccttcgtggc tgtagtaaaa
    17941 gaccggtaat cccagcttaa ggcgtagcgc cagtaagcgg gcaaaaaagc cgaataccag
    18001 ggtggagata attaccacat catggttaga aacatagtgt tgcagcgcga tgtacagcac
    18061 ggcggaggca aatgagacac cggcatacaa ctctttctgg aataccagtg ggatacgttt
    18121 acagaacata tcgcgcaaaa cgccgccaaa cacgccggtg gttaccgccg cgacaacggc
    18181 aataattggg ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag aaaagaccac
    18241 cagtccaagc gcatcgagca ccagaaacac tttgcgcagg taaggcatta caggagcaac
    18301 gatagtggtg agtactgcgg cggtagcgac gataatcaca tattccgggt gtttgaccca
    18361 gccgagcgga tagtggccca gcagaatatc gcgcactgac cctccgccaa ttgcggtggc
    18421 ggtcgcaata ataattacgc caaatgtatc catgcggcgt cgcccggccg ccagagcccc
    18481 cgtcatggct tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca tggttctctc
    18541 cctaaaaatc tgtctggcag ggtagcgatt tgtgcgtatt gtcacgattg agatttttta
    18601 aggtgagagg aaattgataa cttgtagtta tgtgaaatcg ctattttctg tagcgtagat
    18661 gcattcttct gacttcttgt ctgagaaaat gcataagaaa aaataggtta aaattgaggg
    18721 gtggaaaaga aaggtgcaat gtttagccat tgtgttcacc gcacctggac tttggcttac
    18781 gcatcgggta tctcaatcaa caaaaagagt ttttagtttc tgctatttct tctctgtaat
    18841 ttttacactg ttttccaact gcttgatgct gacctgcggg gtcggcaact cctcaggcat
    18901 ggttccgcca agttcctgaa tggtttgcct caccttgcta cccacgtcaa agtgtgtggt
    18961 attagcttgc tgttttgaat tcacgccatc ccgcttgagt ttttcttctg tttgggtagc
    19021 tcgaaagaga ttagccgcca gttctgttga acccatatga tccaggatct tctgactctt
    19081 tttcagcccc ttcagttggt ggatggcttt ctgatctaat ccaccataca gcccctggta
    19141 accatgattc tgaaatatgg cgaaatcagt agctgtcgct accccagctt gctgtgctgc
    19201 ctcaaccaat tgtttgttat gttctttcaa ctcatttctc agaaacagac gcttttcgtc
    19261 ttcacgaagt tgcttaaatg cttcatcgtc agcaagctcc tgccgtcggg tctggatagc
    19321 aaaataagtc tgccctgccg caatgaccgg tttcgcaggg tcgccgtttt gtactaccag
    19381 gtaacaggca tagcgggaga gatgaacatc ttcaagttct cgttgagccc ctgaacctaa
    19441 tacaaccatt ttggtggttt caacgaaatg gtcagatgca gcttgattgc tggcttcgca
    19501 cgcttgagtc gcgcgggcca gtaccttttg aaaattgcgc cagtcccgat aatccagtaa
    19561 cggtgccaat tctctagctg accaaaattc ttgcccttca gtgccataat gccgtatttc
    19621 ttcaaaaggt tgatgatgtt cgttcattgc ccactccatg tgatatcgat tgcaaagaaa
    19681 gtaaatctgt aactgtattt atatacagtt gtgagtatct cacgttctca gtgtgctggg
    19741 caaaaaaaca tcgcaaaatc tggagcaggc acgcaaaaca gaattgaagt tgcggggaag
    19801 aaaaatgccg ctctgtttga gtgggcggca tttgtgaagg ctgaaaagta agaattagtg
    19861 ttacgagtta ctcaatattc tgaatctgct cgcgcatctg ctcaatcaac actttcagct
    19921 cgatggcgga gtttgtcact tcggcattga tagatttcga cgcaagagtg ttcgactcgc
    19981 ggttgaattc ctgcatcata aagtccagac gacgaccaac ggcttctttt ttcttcagaa
    20041 tgttgtaggt ctctttgaca tgcgcttcca ggcggtccag ttcttcggca acgtcaattc
    20101 gttgtgccag cagaaccagc tcctgctcca gacggttgtt ttccaactga acctgagcat
    20161 cttccagctt cgcgaccaga cgctcacgct gccattgcag gatttccggc atatgggcgc
    20221 ggactttgac cacttcggcg gtgacgcctt ccagacgctg ctcgatcaat gctttcagcg
    20281 cctgaccttc ggtttcgcgc gcgacgataa agtcgtccag cgtaccatcc aacgccgcga
    20341 gaatttcagc ggcaatcgcg tcgagatcct gctcctgggc tgccatcacg cccggccagc
    20401 gtagaatatc aaccgggttg atttcccctt cgtcactctg catttttacc cagttcgcgg
    20461 cagttaccag ctgtttagcc agtttttcat tgaggatcag ctcaccttgt gcgctaacat
    20521 ctggctcata gcgcagggta cattccactt taccgcgcgt caggcgagaa cgaatacgct
    20581 cgcgaacgac aggttcaagg ctacggaact gctccggcag acgaaagtaa gtttccagat
    20641 aacgctggtt taccgagcgc atttcccagg ttgcgctccc ccattcaccc ttgatttcac
    20701 gccgggcgta ggcggtcata ctgcggatca tagacgttcc tgtttataaa aggagaggtg
    20761 gaaggattat agccatcgat gccttgtcag gataggaata accgccggaa gtccgtataa
    20821 tgcgcagcca catttgtttc aagccggaga tttcaatatg cgtccagcag gccgtagcaa
    20881 taatcaggtg cgtcccgtta ccctgactcg taactataca aaacatgcag aaggctcggt
    20941 gctggtcgaa tttggcgata ccaaagtgtt gtgtaccgcc tctattgaag aaggcgtgcc
    21001 gcgcttcctg aaaggtcagg gccagggctg gatcaccgca gagtacggta tgctgccgcg
    21061 ttctacccac acccgtaacg ctcgtgaagc ggcgaaaggt aagcagggtg gacgcacaat
    21121 ggaaatccag cgtctgatcg cccgtgctct tcgcgcggca gtagatttga aagcgctggg
    21181 tgagttcacc attacgctgg actgcgacgt gcttcaggct gatggtggca ctcgtaccgc
    21241 gtcgattacg ggtgcctgcg tggcgctggc agatgcgcta cagaagctgg tggaaaacgg
    21301 caagctgaaa accaacccga tgaaagggat ggtagccgca gtttctgtcg gaattgtgaa
    21361 cggcgaagcg gtttgcgatc tggaatacgt tgaagactct gccgcagaga ccgacatgaa
    21421 cgtagtgatg accgaagacg ggcgcatcat tgaagtgcag gggacggcag aaggcgaacc
    21481 gttcacccac gaagaattac tcaccttgtt ggcgctggcc cgagggggaa tcgaatccat
    21541 tgtagcgacg cagaaggcgg cgctggcaaa ctgattttta aggcgactga tgagtcgcct
    21601 tttttttgtc tgtagaaaag taagatgagg agcgaaggca tgaaaccata tcagcgccag
    21661 tttattgaat ttgcgcttgg caagcaggtg ttaaagtttg gcgagtttac gttgaagtcc
    21721 gggcgcaaaa gcccctattt cttcaacgcc gggctgttta ataccgggcg cgatctggca
    21781 ctgttaggcc gtttttacgc tgaagcgttg gtggattccg gccttgagtt cgatctgctg
    21841 tttggccctg cttacaaagg gatcccgatt gccaccacta ccgccgtggc gctggcggag
    21901 catcatgacc ttgacctgcc gtactgcttt aaccgcaaag aggcaaaaga ccacggtgaa
    21961 ggcggcaatc tggttggtag cgcgttacaa ggacgcgtaa tgctggtaga tgatgtgatc
    22021 accgccggaa cggcgattcg cgaatcgatg gagattattc aggctaatgg cgcgacgctt
    22081 gctggcgtgt tgatttcgct cgatcgtcag gaacgggggc gcagcgagat ttcggcgatt
    22141 caggaagttg agcgtgatta caactgcaaa gtgatctcta tcatcaccct gaaagacctg
    22201 attgcttacc tggaagagaa gccggaaatg gcggaacatc tggcggcggt taaggcctat
    22261 cgcgaagagt ttggcgttta aagaaactcg ccggatgaaa agtcatccgg cgtcatatta
    22321 ctgcaactgc gcggcaatta gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt
    22381 aaattcgctg cggacaaaac gtgacagcat accttcacag aaggccagga tctggcttgc
    22441 cagcagggtt tcatcggtgg tgtaaccttc accctcacgc attctctttt cacgcaatac
    22501 ctggcgcagc tgcgcttcaa tacgctcgaa cagctggttg atgcgccctt gcaggcgatc
    22561 ctgttcaaac attagcgcat gaccagtgag gatgcgggtc aggccaggat tacgctcacc
    22621 aaaaccgaga agcagcaaca caatcagacg caggcgcgct gtggtgtctt tctcatcttt
    22681 cagaatcagg ttgatgcgag taatcaggct atcttcgata aactcaatca ggctatcgaa
    22741 catgcgggtc ttactgggga agtggcgata cagtgccgct tcggaaacgc cgacagaggc
    22801 ggccagtttt gccgtcgtga tacgttggct tccatcgctg gattccagca tcagcgccag
    22861 agactgaagt atttcctcgc gacggttcct tttcgcagtt tgtttttctg ccatgttaca
    22921 aaatacccct gaaaataagc acttgccagg cggcacccac gctatgaccg caaacgaaat
    22981 gtttgcggct atgttatgac gttattcgga tgcgtatgtg ttactgacga ccagagtgac
    23041 caaagccgcc ttcaccgcgg tcggtggcgt cgaaatcttc caccagatta aattcagcct
    23101 gtactaccgg aacaaaaatc atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt
    23161 cctgatcacg gttccacacg gaaatcatca actggccctg atagtcagaa tcgatcaatc
    23221 ctaccaggtt accaagcacg ataccgtgct tatgtcccaa tccggagcgc ggcagcatca
    23281 ttgccgccag tgaaggatcg gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt
    23341 cacccggagc cagttctacg gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc
    23401 cagaggtggc ataagtcggg agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa
    23461 cgtcgatttt tttcatcata acgggtcacg atctcgtcga gtaataattg gccaaggagc
    23521 tctttgcgct caagcggtaa gactttatct ccgtcctgcc agaaaaggtg taatgcgttg
    23581 ttgtcgctgt taaatccttg agttggctgg gaaacatcgt tcgcgcagat cagatcaagg
    23641 tttttacgga tacgtttttg ccgggcgtat tcttccacat tatttgtttc ggcggcaaat
    23701 ccaacgacgt agggtcgatg gtcttttagt gcggcaacgc ctgcgacgat atcggggttt
    23761 ttaaccattt ttattgttaa ttcatcaccc tgcgtggcct gctttttgat tttctctggg
    23821 gccacggtag ctgcgcgata atccgccacg gcggcgcagc cgataaaaat attttgctgc
    23881 tgtacagaag cattcacggc ggcttccatt tccagcgcgg tcatcacatc aacacgttta
    23941 acaaacggtg gcgtcggtag tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga
    24001 cgggcagcgg cggcggcgat agcaaaaccc atcttgccgg agctgtgatt agagatataa
    24061 cgcacgggat cgagcggttc acgcgtcggg ccggcggtaa tcataatgtt cagatgtttc
    24121 aggtcgttga cgggcgaaaa atgcgctacc gccatatcca caatggttaa cggatcgagc
    24181 attcgcccag gaccgatatc accacaagcc tgactgccac tgtctggccc ccagatgagc
    24241 aaaccacggg aagcaagcac ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc
    24301 tggttcatgg cggggagcac ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc
    24361 aggtcattcg ccattccggc agcaacacgg gcaatcaaat ctgccgtggc aggggcgaga
    24421 atcactaaat cagcccattt acccagctca atatggccca tagcggcttc ggctgccggg
    24481 tccagcagac tgtcggaaac gggataacca gaaaccgcct gcaagctaag tggggtgata
    24541 aaggcttttg ccgcttcggt catggctacg cggacgtcgg ccccgcgatc gcgcaaacga
    24601 cgcaccagtt caggggtttt ataggcagca ataccgccgc taacgccgag aacgattttt
    24661 ttaccggcca ggctcatgat gatttttcct gttgggtgac accagaagtt ggcgatttta
    24721 tcacaatact tttgttgtcg tgccttcacc tgagaatcac tttgcgaggc gctttccagg
    24781 attgaaaact ggccgtcgat ttaacggaac ggctatgaca ggatgcgagc accacaaagg
    24841 aggtgaaggt gaaaaacaat tcacagctga tgatgccgcg cgaaaaaatg ctgaagtttg
    24901 gtattagcgc cttaacggat gtcgagctgc tggcgctatt tctgcgtacc ggaacgcgcg
    24961 gtaaagatgt attaaccctg gcaaaagaga tgctggagaa tttcggctct ctttatggct
    25021 tgttaacctc tgaatatgaa caatttagtg gcgttcatgg aattggcgtg gcgaaatttg
    25081 cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag
    25141 aaagcccttt actcagcccg gagatgacga gggaattttt acaaagccag ctcacgggtg
    25201 aggagcggga gatctttatg gtgatctttc tcgactccca acaccgggtt ataacgcata
    25261 gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg
    25321 aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg
    25381 ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca
    25441 tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg
    25501 aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact
    25561 tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt
    25621 tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga
    25681 ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc
    25741 gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga
    25801 acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg
    25861 agaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag
    25921 gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag
    25981 gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac
    26041 tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa
    26101 aaaattcgat ccagttgttc gtcagcacgt gatctacaaa gaagcgaaaa tcaaataatt
    26161 ctcgctttga tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc
    26221 catattgatt gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa
    26281 ccagccgtcg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcagtggtgc
    26341 gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag
    26401 tgcttagcgt gcagcggcgg gctaaatatt tgctgctgga gctgcctgag ggctggatta
    26461 tcattcattt ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa
    26521 agcatgacca tgtggatttg gtgatgagca acggtaaagt gctgcgctac accgatccgc
    26581 gccgctttgg tgcctggctg tggaccaaag agctggaagg gcataatgtg ctggcccatc
    26641 ttggaccgga gccgctcagc gacgatttca atggtgagta tctgcatcag aagtgcgcga
    26701 agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga
    26761 atatctatgc cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat
    26821 cactgtcgct ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt
    26881 cgattgagca gggtggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct
    26941 atttcgcgca ggaattgcag gtttacgggc gaaaaggcga gccgtgtcgg gtgtgcggta
    27001 cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga
    27061 agtaattcat gcgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca
    27121 tcagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca
    27181 cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac
    27241 tttccagttc cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat
    27301 ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg
    27361 ccatcaaatc actaaacccg accacttcca cgttccccag atgcgcggtt gcctgctgtg
    27421 ccagcgccac acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg
    27481 ccagaataac gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg
    27541 taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt
    27601 ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga
    27661 tacagtactt caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa
    27721 gaggacacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc
    27781 tccagccgcg cgcaaatgtc tttaaagtta aaagtatgcg gccccatcaa taccggaata
    27841 gcgtgtgcgg cagcttccag cggattatgc ccaccacgtt caaccagtga accgccaaca
    27901 aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca
    27961 acctgcgtgc tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc
    28021 tggcggacaa gattaatcgc atccgggaag cgttctggat gacggggtac caggatgagc
    28081 aataaattcg ggaattgctg taacaatgcc tgatgtgcgg caatgaccac gctctcttca
    28141 ccttcgtgag tgctggtggc aatccatacc gggcggtgtg gtgcccactg gcggcgcagc
    28201 gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta
    28261 acggtcacct gattattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt
    28321 tgcgcagcaa tcagcgtaat acgacgcagc aagcgacgga cgaatttacc cagtttggca
    28381 taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt
    28441 ttgtgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagtttg
    28501 gggtcgactt tattcaggaa acggttgagc gcatcgggca ggtcatacgg cagataaacg
    28561 tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt
    28621 gtaacggtaa tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc
    28681 gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg
    28741 cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc
    28801 acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat
    28861 tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg
    28921 ctttaccatt acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg
    28981 aacatctata ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga
    29041 aggaatcgca gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc
    29101 tttattgaaa atcaacaatg tgcttaaaaa tcattcgggt cggattgccg cgaaagaagt
    29161 gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg
    29221 gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt
    29281 gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta
    29341 ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg
    29401 ctgctttatc aggacaccat cccgatcctg tctgaaaatc cagagattaa cgcgctctac
    29461 ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc
    29521 aaggtattac gtgccaataa gtatgacctt atcgtcaatc ttaccgatca atggatggtt
    29581 gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg
    29641 cagtctgctt tttggcgtaa aagtttcacc catttggtgc cgttgcaggg tggaaatgtg
    29701 gtggaaagta acttatccgt gctgacacca ttgggacttg attcgttggt gaagcagaca
    29761 accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt
    29821 gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac
    29881 aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg
    29941 acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag
    30001 acgccaccag taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc
    30061 gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt
    30121 aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg tccctggtca
    30181 aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt
    30241 gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat
    30301 aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata
    30361 aatattttcc ctttggcggt ttgcagcgtg attttatgcg tattgctcag acagtcgccg
    30421 cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat
    30481 ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt
    30541 ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca
    30601 aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtagcgc
    30661 aggaaaaagg ctttttctat cgcctgacgt cacgttatcg ccattatgcc gcctttgagc
    30721 gggcaacctt cgaacagggt aagccgacac agttgctgat gctgacagat aagcaaatcg
    30781 ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga
    30841 tttatcctga tcgtaaatat agccagcagc cagccaatag ccgtgaaatc ttccgtaaga
    30901 agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta
    30961 aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca
    31021 cattgctata tgttgttggg caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac
    31081 gcggcgtgcg cagtaatgtt cactttttct cggggcgcaa tgatgtctcg gaattaatgg
    31141 cggcggcgga tttattactg catcctgcct accaggaagc ggcgggaatt gtgctgctgg
    31201 aagcgattac tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata
    31261 ttgtcgacgc taattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg
    31321 agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc
    31381 gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa
    31441 cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg cggcaaagat
    31501 ccttttgagg aagttaaaac cttgcagggt gaggtatttc gtgaactgga aactcgccgt
    31561 actctgcgct ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc
    31621 ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg cgctgaccgc
    31681 gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca
    31741 tttggcgaaa aaggcatgaa tccgctgacc agaacttcat ttattattac cgaagatctg
    31801 acaccaacca taagtcttga agattacagt gctgactggg cgactaaccc tccagatgtt
    31861 cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg
    31921 ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt
    31981 aaggaagagg agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc
    32041 gttccacgtc gttggcggga taaagatctt attgggcttt atttttcttc gatgaatatc
    32101 ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgccgc cccgcttaaa
    32161 gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc
    32221 agggaaagaa cgattcgaaa atcgttgtaa cccgtaactt atttttgcca aaattttgga
    32281 tacagaataa atatgtctca actcaatgat agtgacatca tccttttt
//
LOCUS       DAPHDX010000042        32078 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPHDX010000042 DAPHDX010000000
VERSION     DAPHDX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.042"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1363
                     /gene="htrE"
                     /locus_tag="RH058_001912"
     CDS             <1..1363
                     /gene="htrE"
                     /locus_tag="RH058_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDV3137503.1"
                     /translation="LGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWNKLFEETS
                     TSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVTVSINQPL
                     KFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDD
                     SVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARLSYSVN
                     TGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLT
                     FSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDL
                     ENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNV
                     IGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRC
                     QIQ"
     gene            1380..1949
                     /locus_tag="RH058_001913"
     CDS             1380..1949
                     /locus_tag="RH058_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3137504.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            1964..2566
                     /gene="yadL"
                     /locus_tag="RH058_001914"
     CDS             1964..2566
                     /gene="yadL"
                     /locus_tag="RH058_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDV3137505.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            2593..3189
                     /locus_tag="RH058_001915"
     CDS             2593..3189
                     /locus_tag="RH058_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3137506.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            3242..4516
                     /locus_tag="RH058_001916"
     CDS             3242..4516
                     /locus_tag="RH058_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV3137507.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            4629..5423
                     /gene="panB"
                     /locus_tag="RH058_001917"
     CDS             4629..5423
                     /gene="panB"
                     /locus_tag="RH058_001917"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV3137508.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            5435..6286
                     /gene="panC"
                     /locus_tag="RH058_001918"
     CDS             5435..6286
                     /gene="panC"
                     /locus_tag="RH058_001918"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV3137509.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(6360..7262)
                     /gene="rpnC"
                     /locus_tag="RH058_001919"
     CDS             complement(6360..7262)
                     /gene="rpnC"
                     /locus_tag="RH058_001919"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV3137510.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            7536..7916
                     /gene="panD"
                     /locus_tag="RH058_001920"
     CDS             7536..7916
                     /gene="panD"
                     /locus_tag="RH058_001920"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV3137511.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(7920..9149)
                     /locus_tag="RH058_001921"
     CDS             complement(7920..9149)
                     /locus_tag="RH058_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV3137512.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLMSNQPQG"
     gene            complement(9213..9653)
                     /locus_tag="RH058_001922"
     CDS             complement(9213..9653)
                     /locus_tag="RH058_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3137513.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(9758..10528)
                     /locus_tag="RH058_001923"
     CDS             complement(9758..10528)
                     /locus_tag="RH058_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3137514.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(10525..11451)
                     /locus_tag="RH058_001924"
     CDS             complement(10525..11451)
                     /locus_tag="RH058_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3137515.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            11560..12222
                     /gene="can"
                     /locus_tag="RH058_001925"
     CDS             11560..12222
                     /gene="can"
                     /locus_tag="RH058_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV3137516.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(12263..12799)
                     /gene="hpt"
                     /locus_tag="RH058_001926"
     CDS             complement(12263..12799)
                     /gene="hpt"
                     /locus_tag="RH058_001926"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV3137517.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            13005..15395
                     /gene="gcd"
                     /locus_tag="RH058_001927"
     CDS             13005..15395
                     /gene="gcd"
                     /locus_tag="RH058_001927"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV3137518.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(15442..16992)
                     /gene="cueO"
                     /locus_tag="RH058_001928"
     CDS             complement(15442..16992)
                     /gene="cueO"
                     /locus_tag="RH058_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV3137519.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEVLKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            17158..17505
                     /locus_tag="RH058_001929"
     CDS             17158..17505
                     /locus_tag="RH058_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV3137520.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            17611..18477
                     /gene="speE"
                     /locus_tag="RH058_001930"
     CDS             17611..18477
                     /gene="speE"
                     /locus_tag="RH058_001930"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV3137521.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            18493..19287
                     /gene="speD"
                     /locus_tag="RH058_001931"
     CDS             18493..19287
                     /gene="speD"
                     /locus_tag="RH058_001931"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV3137522.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(19325..19687)
                     /locus_tag="RH058_001932"
     CDS             complement(19325..19687)
                     /locus_tag="RH058_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV3137523.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(19863..22460)
                     /gene="acnB"
                     /locus_tag="RH058_001933"
     CDS             complement(19863..22460)
                     /gene="acnB"
                     /locus_tag="RH058_001933"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV3137524.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            22815..24572
                     /locus_tag="RH058_001934"
     CDS             22815..24572
                     /locus_tag="RH058_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV3137525.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(24643..26067)
                     /gene="lpdA"
                     /locus_tag="RH058_001935"
     CDS             complement(24643..26067)
                     /gene="lpdA"
                     /locus_tag="RH058_001935"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV3137526.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(26392..28284)
                     /gene="aceF"
                     /locus_tag="RH058_001936"
     CDS             complement(26392..28284)
                     /gene="aceF"
                     /locus_tag="RH058_001936"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV3137527.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(28299..30962)
                     /gene="aceE"
                     /locus_tag="RH058_001937"
     CDS             complement(28299..30962)
                     /gene="aceE"
                     /locus_tag="RH058_001937"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV3137528.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(31123..31887)
                     /gene="pdhR"
                     /locus_tag="RH058_001938"
     CDS             complement(31123..31887)
                     /gene="pdhR"
                     /locus_tag="RH058_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV3137529.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         7959 a         8453 c         7787 g         7879 t
ORIGIN      
        1 tcttggtctg aatacttcag tcggtgcatt ttctttcgat gtgactcact ccaatgttcg
       61 tataccggat gataaaacat atcagggaca aagttatcgt gtttcctgga acaagttatt
      121 cgaagaaaca agtacttcac tgaatatcgc ggcctatcgc tattcgacac agaattacct
      181 tggtcttaat gatgcactaa ctctaattga tgaagtgaaa catcccgaac aagatcttga
      241 accgaaatcc atgcgtaatt actcgcgcat gaaaaatcag gttacggtca gtattaacca
      301 accgctaaaa tttgagaaaa aagattacgg ttcattttat ctttccggaa gttggtccga
      361 ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc
      421 ctggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga
      481 tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac
      541 ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact
      601 caacgtcagt agcagtggct atagtgataa cgctcgcctc agttatagcg taaatactgg
      661 ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga
      721 atcaccgtgg ggaaccctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt
      781 gtctctcagc accgacggtg gttttgtatt gcatagcggc ggcctgacct tcagtaatga
      841 cagttttagc gactccgata cactggcggt agttcaggct ccaggtgctc aaggagcgcg
      901 aataaattat ggcaacagta ctatcgatcg atggggttat ggtgtcacca gcgctctttc
      961 tccttatcat gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg atgttgaatt
     1021 aaaaagtacc agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg ctgattttga
     1081 aaccgtgcaa gggcaatcag ccattatgaa catcacacga agtgatggta aaaatattcc
     1141 atttgctgca gatatttatg atgagcaagg caatgtcatt ggtaatgttg gacagggtgg
     1201 acaagcattt gttcgtggca ttgagcagca gggaaatatc agcattaaat ggctcgaaga
     1261 aagtaaaccc gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag aaaaaatagc
     1321 gcaatctatt attctgaatg gaattcggtg tcagattcag taactacaag ggacgtcaaa
     1381 tgataaaaat aacgccacat aaaatagcga tactgatggg aatattatta tcaccatcag
     1441 tatttgcaac ggatattaat gtagagttta cagccactgt caaagcgaca acctgtaaca
     1501 tcacacttac tggtaataac gtcacgaatg atggcaataa taactacaca ttgagaatcc
     1561 ctaagatggg tctggataag atcgcgaata aaacgacaga atctcaggct gattttaaac
     1621 tggttgctag tgggtgcagc agtggcatca gttggattga taccactctg accggaaatg
     1681 catcatcaag ctcacctaag cttattatac cgcagtctgg tgattcatct tcgacgacaa
     1741 gtaatatcgg tatgggtttc aaaaaacgga ctactgatga tgccactttc cttaaaccta
     1801 acagtgcgga aaagatacgc tggagcacag acgagatgca gcccgataag ggtcttgaaa
     1861 tgaccgttgc gctacgtgaa acagatgcag ggcaaggcgt accggggaat tttcgtgcac
     1921 tggcaacgtt taatttcatc tatcaataag aaggataatt aagatgacat ttaaaaattt
     1981 acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta
     2041 tgcggcaaca gacagcattg ggctgaccgt gattactacc gtagaaatag gtacttgtac
     2101 cgctacatta gtaaatgact ctgatcagga catttctgtt gttgactttg gcgatgtata
     2161 tatttctgaa atcaatgcca agaccaaagt taaaacattc aaactcaaat tcaaagactg
     2221 tgcgggtatc cccaataaaa aagcacaaat aaaattaacc aagcgagcca catgcgaagg
     2281 tactgctaat gacggtgcgg ggttcgcaaa tggatccaca gccacagata aagcaagtgc
     2341 cgtcgccgtt gaagtctgga gcaccgaaac tccggcaaca ggtagcgcaa cacaatttag
     2401 ttgtgtgaca ccagcatctc aagaggtcac tatctccaat gcagccaacg cggttgttta
     2461 ttatccgatg agtgctcgac tggtcgtcga aaaaaataaa accgtaagca atgtcactgc
     2521 gggtaaattt tctgcacctg ccacatttac agtaacctat aactaacaga ctgacagatc
     2581 caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg
     2641 tcactacagt ttcccattac ctatccagcc atcgctgggc aggatattga tcttgttgcc
     2701 aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc
     2761 gtcgttgggg tgggatattt ttcagataat gttactcctg aaagctatca accaggtgga
     2821 aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgcttaat
     2881 cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata
     2941 tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag
     3001 gacccgacca atatattcaa tgttattagt tcagcaggaa attctcgttc cgtgtatcca
     3061 gtaatgactt ctgccctgca taattcttca tggaaatttt atgccagaat gcaaaaaatc
     3121 gatcccgcgc tggatgtcat atctggccag gtaatgagtc atatattagt tgatgtgtac
     3181 tacgaataat agatcaaaac aaataactgc gactgttaca gcagtagatg gtggagtgat
     3241 aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgagtt gttataccgc
     3301 gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg gcgttggtcc
     3361 gtcagatcaa aaatggagag aaacggggcc gaatggcgat gctactgtca tattcagata
     3421 tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt
     3481 aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag
     3541 aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa
     3601 aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt acacattatt
     3661 aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac ctgggatata
     3721 cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag acgtgttata
     3781 ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg gtatttatca
     3841 gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa ctcaacagat
     3901 caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg caggccaagg
     3961 tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg tcaagttgac
     4021 taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt ctacggtaaa
     4081 aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc cttttgatat
     4141 ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt caacaaaggt
     4201 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg cagcaaaagg
     4261 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
     4321 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
     4381 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
     4441 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
     4501 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
     4561 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
     4621 gacacgttat gaaaccgacc accattgctt cacttcagaa atgtaagcag gataaaaagc
     4681 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaaggac
     4741 ttaacgtcat gctggtaggc gattcgctgg gcatgacggt tcagggccac gactccaccc
     4801 ttcccgttac cgtcgaggat atcgcctatc ataccgccgc cgtacgtcgc ggcgcgccaa
     4861 actgcctgct gttggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
     4921 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
     4981 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
     5041 gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg
     5101 aagcaggcga tcaactgctc agcgatgcat tagccttaga agccgctggg gcacagctgc
     5161 tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca ctggcgatcc
     5221 cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgacg
     5281 ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     5341 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     5401 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     5461 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     5521 tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     5581 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     5641 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     5701 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc atacttacgt
     5761 tgacgttccc gggctttcga ccatgctgga aggcgcgagc cgtccggggc attttcgcgg
     5821 cgtttcgact atcgtcagca agctgtttaa cctggtccag ccggacatcg cctgcttcgg
     5881 tgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg gttgccgata tgggcttcga
     5941 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     6001 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     6061 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     6121 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     6181 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     6241 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     6301 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     6361 taaataaggt taattacctt atcaatctca gcaagagata aatttgtcat ctctgcaacg
     6421 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     6481 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     6541 atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     6601 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     6661 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     6721 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     6781 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc
     6841 gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     6901 aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg
     6961 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     7021 tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc
     7081 gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     7141 aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     7201 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     7261 atcttccctg tcttccgtgt tgtcttgcgt gaaggcagta tccagtcaag gcgcgccgtc
     7321 gtcagccgcc tctttagtgt ttaccgagca gcgttcagag aaatttcaat tcgttacagg
     7381 cgatacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     7441 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     7501 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     7561 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     7621 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     7681 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     7741 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     7801 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     7861 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     7921 tatccctgcg gctggttact catcagccgc gacatcgtct ccagcgaatc cgttcttaag
     7981 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     8041 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaatccg
     8101 ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa
     8161 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgatag
     8221 ccatccaccc gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc gcgaatttcg
     8281 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     8341 ttgatgcgcg aggtaacaac aaacgccgtc gccttcatgc catattgttt caatacagga
     8401 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     8461 gggagattga tcttattctt cacgtaacct tccaactgcg ccatgctcag tgtcgcgtat
     8521 cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     8581 gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt tagcaccggc
     8641 aggccattat cgggttgggc atccagcgcg ctgatatagg cgagtcgatc gccaatacgg
     8701 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     8761 ttgtccgcca gtaccccaaa tggcgcactg cccgcactcg gcgcgttata gaccggcgta
     8821 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     8881 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     8941 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgcccg
     9001 gcccgaatat tccccacggt caccatcttg tcgccaattt gcgcccagat cgcagcattt
     9061 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     9121 agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa acaacgcttt
     9181 tattgtagca aaagcgctat aagaatcgtg ggttaatact catacaaatt atgtttgagg
     9241 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     9301 tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     9361 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     9421 aatgaagcca cgcggtacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     9481 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaac
     9541 ccacgccaga aattcacggc ccggcactga agaagcgccc catgtttttt ctccagcgca
     9601 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     9661 ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     9721 actcctctcc cccaaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     9781 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     9841 aggtagtgac cagcggaaca tcattgatac cgaggaagcc gtagcggaaa ccactgatca
     9901 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     9961 agtaaaagac cccacccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
    10021 tgtcatcgaa cgttttggca aacacaccgt tcagcaaacc cgcaagggag aacaacaccg
    10081 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
    10141 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
    10201 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
    10261 gctggaactt ggcaccaaaa aatgatgacg caacgttggc gtaggcattg gtgatcaccg
    10321 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
    10381 tacgcgaacc aatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
    10441 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
    10501 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
    10561 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
    10621 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt caacttccag
    10681 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
    10741 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
    10801 attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca tttctgcttc
    10861 ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
    10921 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
    10981 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
    11041 taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agttgtttaa gatatttttc
    11101 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat ttaccacaat
    11161 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
    11221 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
    11281 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
    11341 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
    11401 gccgcctgga taagtttttt taagctgttg aagttccagt gcaatggtca taaattttta
    11461 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
    11521 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
    11581 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
    11641 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
    11701 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
    11761 aacctggtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
    11821 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgca
    11881 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
    11941 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
    12001 gaactgaacg tcatggaaca ggtttataac ctgggccact ccaccattat gcaatcagcg
    12061 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
    12121 ctgcgtgatc tggatgtcac agcgaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
    12181 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
    12241 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg atatacggca
    12301 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
    12361 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
    12421 tacaaatcgc cagcgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
    12481 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
    12541 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
    12601 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
    12661 taaatgagcc acgcagcaga ccaaccagca ccatatcgct gccgctgtct ttgtaacgct
    12721 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
    12781 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
    12841 gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt
    12901 cgcggaatct gttaataaag ctcgaattgt gatgacgatc acacatgtta aaacctataa
    12961 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
    13021 caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
    13081 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
    13141 ctggtcttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt
    13201 ggctgtacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
    13261 acttctgggc gctgactccg cgcagcgaca ttctggtctt cttcggcatc tggctgatcc
    13321 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
    13381 tggcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaaa
    13441 tcaacggcac cttaagcgcc aatgccacac ctgcggaagc tatctcaccc gttgccgatc
    13501 aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa
    13561 ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact ggcgatgtga
    13621 agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa gtgggcgaca
    13681 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag
    13741 agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac gtaacctgcc
    13801 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc accggaagtg atggcggatt
    13861 gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt aacgctgaaa
    13921 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
    13981 cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc gataaaacca
    14041 tcgtgatggc cggttcagtt accgataact tctcaacccg cgaaacgtct ggcgtgatcc
    14101 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc
    14161 cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac tcctgggcac
    14221 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    14281 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    14341 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    14401 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    14461 cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt cgcaatggcg
    14521 aactggtggt tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg
    14581 ttactccgac ccagccgttc tctgaactga gcttccgtcc gaagaaagat ttgagcggtg
    14641 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    14701 tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc ttcccgggta
    14761 acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    14821 ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg ggtaacccga
    14881 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    14941 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg ccgtgtaaac
    15001 agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga
    15061 aacgtattgg tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg gtgccgttca
    15121 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    15181 ctgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    15241 agggccgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg aatggcaagc
    15301 agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    15361 ttgtggctta tgcgctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    15421 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    15481 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    15541 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    15601 agccgctggc ggtttgccat tttctgacaa gatacggaac tgggtgccgt gaatatggaa
    15661 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    15721 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    15781 gaaatcgaac ttcccgccgt ggttcatatg attcatatta ccgtgcccca tatggcccat
    15841 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    15901 catacccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    15961 ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaggcact
    16021 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg caatcgccat
    16081 ccccatctgg ctgaccggta gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    16141 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    16201 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    16261 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    16321 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    16381 ggtcatcaca tccagttgat aatcaatctg cccatcagcg ttaaattttt tatcctgaac
    16441 aatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggacctcgtc
    16501 atcttcaatt accaccagcc cagccaaccc catcgccacc tgtcgcccgg tcttgccatg
    16561 ttgatgtgga tggaaccagc aagtagcggc aggttgatca acgttcaacg tcaccgagcg
    16621 cttgccacct ggcagaataa ttccctgcgg gccgccgtcg acttcacccg gtacttccag
    16681 cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc
    16741 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    16801 tgcagttttc tcgccaaagg tagactgacc tgcgccaata gttaactgaa tgcgattacg
    16861 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    16921 gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt ttaagaaatc
    16981 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    17041 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    17101 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    17161 aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac
    17221 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    17281 aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt
    17341 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc
    17401 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa
    17461 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    17521 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    17581 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    17641 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    17701 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    17761 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    17821 ccgctactgg cccatggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    17881 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    17941 gcgggtgtcg tatcgttctg ccgtcagtat ctacccaacc ataacgccgg tagctacgac
    18001 gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    18061 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagccttttc
    18121 acttcggcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    18181 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    18241 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    18301 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    18361 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    18421 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    18481 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    18541 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    18601 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    18661 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    18721 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    18781 caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta
    18841 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    18901 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    18961 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt
    19021 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    19081 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    19141 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    19201 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    19261 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    19321 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    19381 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    19441 cagcgaattc cagttgattg gcgcgaacca tcacctcttc accgtccatc cacagggtgt
    19501 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    19561 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    19621 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    19681 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcattgc gccattgatg
    19741 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa
    19801 atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact
    19861 tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg agaaagctgg
    19921 ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc cacgtaggtc
    19981 tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc cagttccgca
    20041 gaagccagga agacattcgc gccagtaccc agacggttcg ggaagttacg ggtagaggtg
    20101 gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag agaacagccc
    20161 gggatctcga tacgcgcacc actcttaccg aagacgctgt agtagccttc ttcagtcaac
    20221 tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcag ttggcctttg
    20281 tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat gcaggaaccg
    20341 ataaatactt cgtcgatctt ctcaccctgt accgcagaca gcggacgcgc gtcatccggg
    20401 tcgttcggtg cacagaggat tggctcttta atatccgcca gatcgatgtc gatcactgcc
    20461 gcgtattcag catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg
    20521 ccctgaatac gacgttccag ggtgcgacga tcgccgtaac cttccgcgat catccacttc
    20581 agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag cttgatggta
    20641 caaccagcgg cagaacgctc ggcggacgca tcggtcagtt caaatgcctg ctcaactttc
    20701 agatccggca gaccttcaat ttccaggatg cggccagaga agatgttttt cttgcctttc
    20761 ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcatg taccagatcg
    20821 cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga ttccggcata
    20881 tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa
    20941 gagataccga tcgggaaacg ggtatgggaa tcaccaccgg taccgacggt atccggcagc
    21001 agcatacggt tcagccagga gtgaatgacg ccgtcacccg gacgcagcga cacaccgcca
    21061 cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac
    21121 gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg
    21181 tctttcagtt catcacgggt catcgggccg gtggtgtcct gagagcctac agaagtcatt
    21241 ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt
    21301 ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca
    21361 tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg cccgataatc
    21421 agcgggatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag
    21481 gtcgccagca gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca
    21541 atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc acccgcgtct
    21601 tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt
    21661 ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat
    21721 ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa acctttctgt
    21781 tgcagagctt caatctgttt gatcgggcca acaacacctg gctggtctgg ctcaatacct
    21841 tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg cgaccacgca
    21901 tccggtgccg gagaaaggtc atcggtgttg gtttcgccag tgactttgaa gacggtaacg
    21961 gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc ccaggactgc
    22021 attacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc atagaagtta
    22081 tcgaacatca gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca
    22141 tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc cagcagttcg
    22201 atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat agcagccagg
    22261 aagcctgctt tgacataggc ggcttcatcg acgccagggg gaacacggtt ggttaacaga
    22321 tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac aagtgcggcc
    22381 atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc
    22441 ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg
    22501 attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg
    22561 gcagaatagc aggattttga cgatgtgtta atctgtttac aaaaaagcaa cataaaaaag
    22621 tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc
    22681 gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc
    22741 ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc
    22801 ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt
    22861 gcagtgccgg gctttgtgcc gcctctaccg ggctatatat aaaaagccgc acagtggaag
    22921 cgcctgtgga accgcaatcg acacaacaga ctgcgcctga catcaccgca gttacgcttc
    22981 ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt caaatccgca ttcagcactg
    23041 cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc cgattctctg ctttcacaag
    23101 tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata
    23161 atccacttaa acaaggcgat gctgctattc aggcagtatc tgaccagccg tgggacgcca
    23221 gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa aacccgcaat
    23281 gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg gactcggtac
    23341 agcgattgcg gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc gaacagaaag
    23401 ttattaccac aacgaagaaa gctgtaccgg taacacagac agtcacggct cccgtcatac
    23461 catccaatac cgtttcaact gccagcccca tcattacaga gcctgcaaca accgtcattt
    23521 ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt
    23581 acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca gccggagaac
    23641 cgtttattga cagctttgtg cgcggattcg gctatagcat gggtgttgct accacgtacg
    23701 cactattcag cagcatcgac tgggacgacg acgatcatga ccatcatcat catgacgatg
    23761 atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg
    23821 acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat cttactgata
    23881 agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat catgatcagg
    23941 atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc acgcagctac
    24001 ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag caacgaacac
    24061 acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg
    24121 aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc caacccctga
    24181 cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc cgcctcgcct gagcagcgcc
    24241 aggcggttcg cgagaaaatg cagactaacc cacagaacca gcagcgaaga gaggcagcgc
    24301 gtgagcgtat tcagtctgca actcccgaac agcgccaggt atttaaggaa aaagtacagc
    24361 agcgcccact gaaccaacag caacgtgata acgcccgcca gcgtgttcaa tcagcatcac
    24421 ctgaacaacg tcaggttttt cgggaaaaag ttcaggagag ccgcccacaa cgtctaaacg
    24481 acagtaacca tactgtcagg ctgaataacg agcaacggtc agcagtacgc gaacgtctct
    24541 ctgagcgcgg agcaaggcga caggaaaggt aaattgcaga cgtaaaaaaa gcggcgtggt
    24601 tagccgcttt tttaattgcc ggatgttccg gcaaacgaac aattacttct tcttcgcttt
    24661 cgggttcggc aggtcggtaa tgctaccttc gaacacttct gccgccaggc ccacagactc
    24721 gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct tcagcatcac aacccatttc
    24781 gattgccagg ccgatttcac ccagcagctc gccgccgttg gtaccgacaa tcgcaccacc
    24841 gatcacacgg tgagattctt tgtcgaaaat cagcttggtc ataccgtctg cgcagtcgga
    24901 agcgatagca cgaccagaag cagcccacgg gaaggtggcg gtttcatagc tgatgccttt
    24961 ctctttcgct tctttctcag tcagacctac ccatgcaact tctggttcgg tataggcgat
    25021 ggacgggata actttcggat cgaagtagtg tttcttaccg gcgataactt cagcggcaac
    25081 gtgaccttcg tgaacacctt tgtgtgccag catcggctga ccgacgatat cgccgatagc
    25141 aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac cacggtcgtc
    25201 cacttccacg ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac gaccaatcgc
    25261 taccagcacg gcgtcgtaac gctgcggttc agcgggtgct tttttgcctt ccatcgtcac
    25321 ataaataccg tcttctttcg cttcaacggc ggtaactttg gtttccagca tcaggttgaa
    25381 tttcttgctg atacgcttgg tgaagacttt aacgatgtct ttgtcagctg ccgggataac
    25441 ctggtcgaac atttcaacca cgtcaatctg tgaacccagc gcgtggtata cggtgcccat
    25501 ttccagaccg atgataccgc cacccattac cagcaggcgt tctggtactt ctttcagttc
    25561 cagcgcgtca gtggagtccc agatacgcgg atcttcatgc ggaataaacg gcagttggat
    25621 cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg atcacggttt tgccgttctc
    25681 accttcaact tccagggtgt tagccccggt gaatttaccc agaccgttga ccactttgac
    25741 tttgcggcct ttcgccatac cagccagacc accggtcagc tgattgatca ctttctcttt
    25801 ccaggtacga atcttgtcaa tatcggtttt cggttcgccg aagacgatac cgtgttcagc
    25861 cagcgctttg gcttcttcga taacttttgc tacgtgcagc agtgctttag aagggataca
    25921 gccgacgttc aggcaaacac cgccaagggt gttgtaacgt tctacgatta cggtttccag
    25981 acctaaatca gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc caagtaccac
    26041 gacctgagtt ttgatttcag tactcatcat gacctctata tatttatctc cggcggtcat
    26101 acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc cctcacccca gtcacttact
    26161 tatgtaagct cctggggatt ctggcgcttg ctaccttgct gcacccagaa atccataggg
    26221 tatgtgaagt aataccctaa ccaccaccgg gtcgttctat ccgtcggtat tttacaaaat
    26281 tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa taacctcaat
    26341 acattcatga gattaccaga aaaaagccgg ccgttgggcc ggctctttta cttacatcac
    26401 cagacggcga atgtcagaca gcgtgttgtt aatgatggta atgaaacggg caccatcagc
    26461 accgtcgatc acgcggtggt cgaaggagag agaaatcggc agcatcagac gcggcacgaa
    26521 ctctttacca ttccacaccg gctccatcgc ggacttggaa acgccgagga tagccacttc
    26581 cggcgcgttc acaatcggcg cgaagtgggt agtacccagg ccgccgatgc tggagatggt
    26641 gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg tcacgcgctt tcttagaaat
    26701 agtcatcagc tcgcgagaca gctcgatgat gcctttcttg ttgacgtctt tgaataccgg
    26761 aacaaccaga ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt tcttcagggt
    26821 cagacgctga ccgtcttccg acaacgaact gttgaagcga ggcatctgct caagagctgc
    26881 agcaacggct ttcatgatga agacaactgg ggtgatcttc acatccagct tacgtttcgc
    26941 cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg ttttgtcgaa
    27001 gtgagtaaca tgcgggatca tcacccagtt acggctcagg ttcgcaccag aaattttctg
    27061 gatacggccc agttccactt cttcgatttc accaaacttg ctgaagtcca ccttcggcca
    27121 cggcagcatg cctgggatac cgccgccagt cgccgccgga gctgcttctg cacgtttgat
    27181 agcttctttc acgtaagcct gaacgtcttc gcgcaggata cgacctttac ggccagtgcc
    27241 cttcactttc gccaggttaa cgccaaactc gcgtgccaga cggcggatca gcggagtcgc
    27301 gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct tcggctttcg cagccggagc
    27361 tgctgccgga gcttcagctt ttgctgccgg agccggcgct gccgcttcct gtttcgccgg
    27421 agccgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgagc cagttttcac
    27481 tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag ccggaacttc
    27541 catagaagct ttgtcgcctt ctacggtaat cagtgactgt tcagcggcaa ctttgtcgcc
    27601 cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac
    27661 ttctttcacg ccagccgctg gtgcaggggc cgctgccgga gccgcttcct gtttagccgc
    27721 cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg agccggtaga
    27781 cactttgtca cccacgttca ctttgatctc tttcacggtg ccagcaaacg gagccggaac
    27841 ttccatagaa gctttatcgc cttctacggt gatcagcgac tgttcagctt caactttatc
    27901 gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt
    27961 aacgtctttt gccgccgcag ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc
    28021 tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc
    28081 gccggtctgg gttttatcgc caacagagac tttgatctct ttaacgatac ccgcctgcgg
    28141 agacggaact tccatagagg ctttgtcgcc ttctacggtg atcagcgact gttcggcttc
    28201 aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag ccccgatgtc
    28261 cggtactttg atttcgatag ccattattct tttacctctt acgccagacg cgggttaact
    28321 ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt atcgatttcg
    28381 ccacgtttag ccagttcgcc cagcgccgca accacgacat aagaagcatc aacttcgaag
    28441 tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc cagtacgcgg
    28501 tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat atagtcggta
    28561 gatgccactg ccggagcgtc gttcatcacc tgagcgatat acggaacgcg cggagtttcc
    28621 agcggatgaa gcatgttcca gcgttcacaa tcctgaccat cacgcgccag ctcggtgaag
    28681 gaggtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat ctctgccgct
    28741 tcacggacgt gacgcaggat agaaccggag cccagcagct gaactttacc tttgctacct
    28801 tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc ttccggcatt
    28861 gccggcatgt ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttt
    28921 tcaccgtaca tacgctccag accgtcatgc atgatgacag caacttcgta agcgtaagcc
    28981 gggtcgtaag agatacagtt cgggatagtc agcgactgaa tgtggctgtg accatcttcg
    29041 tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat caggaagcca
    29101 cgcgcttgct ggtcgccagc cgcccagcac agatcgccga tacgctggaa gccgaacatc
    29161 gagtaataga tgtagaacgg gatcatcggc agattgttgg tgctgtaaga ggtcgccgct
    29221 gccagccagg aacaacctgc gcccagctcg ttgatccctt cctgcagaat ctgacctttc
    29281 tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc
    29341 gggctgtaaa taccaatctg acggaacaga ccttccatac cgaaagtacg cgcttcgtcg
    29401 gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcattac gttcagggca
    29461 cgaacgaaag cgatagtggt agagatctct ttgctctgct cttccagcag cgcgccgaag
    29521 tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg gctgacggct tggcagataa
    29581 ccgtgcagtt tctgacgctg tgcgtgcaga taggtatgct cttcagaacc ttccgggaag
    29641 gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa acggtcgcgg
    29701 atgtgacgca cgccgtccat gttcattttc ttaacctggt gcgcgatgtt tttaccttca
    29761 gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa ggattactgt cgctttgcct
    29821 ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg accaccacgg
    29881 ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt ttcaggatat
    29941 ttaccgaaga agtgttcacg aacgtacgca ccatctttcg atttgaaggt ctggtagtcg
    30001 ccgtcaacgg tttcgttcat cagctggatc agtttaccgc tggtatcttt acgcagcagt
    30061 tcatcccaac ggctacccca catcactttg atcacgttcc agccagcacc ttcgaagatg
    30121 ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg
    30181 ttacagttga taacgaagac caggttatcc agtttttcac gggtagcgat ggtgatcgca
    30241 cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac ggtctgttta
    30301 gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg gtaaatagca
    30361 ccaatcggcc ccagacccat agatacggtc gggaactgcc agaattccgg catcagtttc
    30421 gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt atccagctgc
    30481 tcctgagtca gacgaccttc caggaaagca cgagcgtaca cgcccgggga gatgtggccc
    30541 tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag
    30601 cacacatcat aaatggttgc ggaagactgg aaggacgcca tatggccgcc cagttcgagg
    30661 tcttttttcg acgcacgcag caccgtcatg atggcgttcc agcggatagc tgaacgaata
    30721 cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg gatggtgttg
    30781 atgtagttgc tgatacctgt gcctgcggct acgtttacac cgcctttgcg ggcttcagca
    30841 agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat gaccgattcg
    30901 atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa acgttctgac
    30961 atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc ccattgaact
    31021 ctcgccggaa agctcaataa gacaggttct acgtttagtt gccgcgcttt tatatgcgct
    31081 tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct ttcgttgctc
    31141 cagacgacgc agagaacgct cacggcggct ctcttcacga cttctgtcga gcaaaatttc
    31201 ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct taccggccat
    31261 aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct cgcgacgcga
    31321 atagagcaat tcgaagttct ggcggacatt ctgggccaac atcggctcca tacaccttag
    31381 cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat actggagtac
    31441 ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg cgtggtggag
    31501 ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat aagcggcgat
    31561 accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gactcaggat ggtcggagag
    31621 cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa aagtgccgcc
    31681 accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga
    31741 gggacgggag acgtcaaact gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg
    31801 gcggagagtg ccttcgagga tcaaaaactc cagttgctgc tcaatcacat cggagagttt
    31861 tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg ccgaagtcaa
    31921 ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac cttatgtcca
    31981 tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac atatataacg
    32041 tttcaaagtt gtaactatgc acaaatgtag actttacg
//
LOCUS       DAPHDX010000043        31754 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPHDX010000043 DAPHDX010000000
VERSION     DAPHDX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.043"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="RH058_001939"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="RH058_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3137530.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1184..1567
                     /gene="secE"
                     /locus_tag="RH058_001940"
     CDS             1184..1567
                     /gene="secE"
                     /locus_tag="RH058_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV3137531.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1569..2114
                     /gene="nusG"
                     /locus_tag="RH058_001941"
     CDS             1569..2114
                     /gene="nusG"
                     /locus_tag="RH058_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV3137532.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2273..2701
                     /gene="rplK"
                     /locus_tag="RH058_001942"
     CDS             2273..2701
                     /gene="rplK"
                     /locus_tag="RH058_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV3137533.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2705..3409
                     /gene="rplA"
                     /locus_tag="RH058_001943"
     CDS             2705..3409
                     /gene="rplA"
                     /locus_tag="RH058_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV3137534.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3822..4319
                     /gene="rplJ"
                     /locus_tag="RH058_001944"
     CDS             3822..4319
                     /gene="rplJ"
                     /locus_tag="RH058_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV3137535.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4386..4751
                     /gene="rplL"
                     /locus_tag="RH058_001945"
     CDS             4386..4751
                     /gene="rplL"
                     /locus_tag="RH058_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV3137536.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            5071..9099
                     /gene="rpoB"
                     /locus_tag="RH058_001946"
     CDS             5071..9099
                     /gene="rpoB"
                     /locus_tag="RH058_001946"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV3137537.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9176..13399
                     /gene="rpoC"
                     /locus_tag="RH058_001947"
     CDS             9176..13399
                     /gene="rpoC"
                     /locus_tag="RH058_001947"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV3137538.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13612..14151
                     /locus_tag="RH058_001948"
     CDS             13612..14151
                     /locus_tag="RH058_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDV3137539.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14561..15694)
                     /gene="thiH"
                     /locus_tag="RH058_001949"
     CDS             complement(14561..15694)
                     /gene="thiH"
                     /locus_tag="RH058_001949"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV3137540.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15691..16461)
                     /gene="thiG"
                     /locus_tag="RH058_001950"
     CDS             complement(15691..16461)
                     /gene="thiG"
                     /locus_tag="RH058_001950"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV3137541.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16463..16663)
                     /gene="thiS"
                     /locus_tag="RH058_001951"
     CDS             complement(16463..16663)
                     /gene="thiS"
                     /locus_tag="RH058_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV3137542.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16647..17402)
                     /gene="thiF"
                     /locus_tag="RH058_001952"
     CDS             complement(16647..17402)
                     /gene="thiF"
                     /locus_tag="RH058_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV3137543.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17395..18030)
                     /gene="thiE"
                     /locus_tag="RH058_001953"
     CDS             complement(17395..18030)
                     /gene="thiE"
                     /locus_tag="RH058_001953"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV3137544.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(18030..19925)
                     /gene="thiC"
                     /locus_tag="RH058_001954"
     CDS             complement(18030..19925)
                     /gene="thiC"
                     /locus_tag="RH058_001954"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV3137545.1"
                     /translation="MSATKLTRREQRAHAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(20005..20105)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20157..20633)
                     /gene="rsd"
                     /locus_tag="RH058_001955"
     CDS             complement(20157..20633)
                     /gene="rsd"
                     /locus_tag="RH058_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV3137546.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20728..21501
                     /gene="nudC"
                     /locus_tag="RH058_001956"
     CDS             20728..21501
                     /gene="nudC"
                     /locus_tag="RH058_001956"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV3137547.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21541..22605
                     /gene="hemE"
                     /locus_tag="RH058_001957"
     CDS             21541..22605
                     /gene="hemE"
                     /locus_tag="RH058_001957"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV3137548.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22615..23286
                     /gene="nfi"
                     /locus_tag="RH058_001958"
     CDS             22615..23286
                     /gene="nfi"
                     /locus_tag="RH058_001958"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV3137549.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            23329..23919
                     /locus_tag="RH058_001959"
     CDS             23329..23919
                     /locus_tag="RH058_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV3137550.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            24106..24378
                     /gene="hupA"
                     /locus_tag="RH058_001960"
     CDS             24106..24378
                     /gene="hupA"
                     /locus_tag="RH058_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV3137551.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24391..25086
                     /locus_tag="RH058_001961"
     CDS             24391..25086
                     /locus_tag="RH058_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV3137552.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(25088..25513)
                     /gene="zraP"
                     /locus_tag="RH058_001962"
     CDS             complement(25088..25513)
                     /gene="zraP"
                     /locus_tag="RH058_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV3137553.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25751..27127
                     /gene="zraS"
                     /locus_tag="RH058_001963"
     CDS             25751..27127
                     /gene="zraS"
                     /locus_tag="RH058_001963"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV3137554.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            27124..28449
                     /gene="zraR"
                     /locus_tag="RH058_001964"
     CDS             27124..28449
                     /gene="zraR"
                     /locus_tag="RH058_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV3137555.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28446..29735)
                     /gene="purD"
                     /locus_tag="RH058_001965"
     CDS             complement(28446..29735)
                     /gene="purD"
                     /locus_tag="RH058_001965"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV3137556.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVETFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29747..31336)
                     /gene="purH"
                     /locus_tag="RH058_001966"
     CDS             complement(29747..31336)
                     /gene="purH"
                     /locus_tag="RH058_001966"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV3137557.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7506 a         8207 c         8689 g         7352 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggtcgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      961 ataacatttg acgcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
     1021 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
     1081 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
     1141 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
     1201 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
     1261 cctggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
     1321 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
     1381 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
     1441 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
     1501 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
     1561 gttctaagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
     1621 ttgaaggccg cgtagcaacg tcgctgcgtg agcatatcaa attacacaac atggaagatt
     1681 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1741 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1801 cgagctggca cctggtgcgc agcgtaccgc gtgtgatggg cttcatcggc ggtacttccg
     1861 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1921 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1981 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     2041 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     2101 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgtga
     2161 gattggaata caatttcgcg ccttttgttt ttatgggcct tgcccgtaaa acgatttttt
     2221 atatcacggg gagcctctca gaggcgttat tacccaactt gaggaattta taatggctaa
     2281 gaaagtacaa gcctatgtca agctgcaggt tgcagctggt atggctaacc cgagtccgcc
     2341 agtaggtccg gctctgggtc agcagggcgt aaacatcatg gaattctgca aagcgttcaa
     2401 cgcaaaaact gattccatcg aaaaaggtct gccgattccg gtagtaatca ccgtttacgc
     2461 tgaccgttct ttcactttcg ttaccaagac tccgccggca gcagttctgc tgaaaaaagc
     2521 ggctggtatc aagtctggtt ccggtaagcc gaacaaagac aaagtgggta aaatttcccg
     2581 cgctcagctg caggaaatcg cgcagaccaa agctgccgac atgactggtg ccgacattga
     2641 agcgatgact cgctccatcg aaggtactgc acgttccatg ggcctggtag tggaggacta
     2701 agaaatggct aaactgacca agcgcatgcg tgttatccgc gagaaagttg atgcaaccaa
     2761 acagtacgac atcaacgaag ctatcgcact gctgaaagag ctggcgactg ctaaattcgt
     2821 agaaagcgtg gacgtagctg ttaacctcgg catcgacgct cgtaaatctg accagaacgt
     2881 acgtggtgca actgtactgc cgcacggtac tggccgttcc gttcgcgtag ccgtatttac
     2941 ccaaggtgca aacgctgaag ctgctaaagc tgcaggcgca gaactggtag gtatggaaga
     3001 tctggctgac cagatcaaga aaggcgaaat gaactttgac gttgttattg cttctccgga
     3061 tgcaatgcgc gttgttggcc agctgggcca ggttctgggt ccgcgcggcc tgatgccaaa
     3121 cccgaaagtg ggtactgtaa caccgaacgt tgctgaagcg gttaaaaacg ctaaagctgg
     3181 ccaggttcgt taccgtaacg acaaaaacgg catcatccac accaccatcg gtaaagtgga
     3241 ctttgacgct gacaaactga aagaaaacct ggaagctctg ctggttgcgc tgaaaaaagc
     3301 aaaaccgact caggcgaaag gcgtgtacat caagaaagtt agcatctcca ccaccatggg
     3361 tgcaggtgtt gcagttgacc aggctggcct gagcgcttct gtaaactaat gcctttacgt
     3421 gggcggtgat tttgtctaca atcttacccc cacgtataat gcttaatgca gacgtatatc
     3481 cgagatattc gggttgtggc aaggcggcaa ctgagtgagt cgccaggagc atagctaact
     3541 atgtgactgg tgcgaatgaa ggaagccaac gccgccacaa gttgaatagc gacggataga
     3601 aaagatttgt tcgttggagc ctggcctatc caggcctccg tcgaagaccg caggagtttc
     3661 gcaagaaact taatcccctg cgtagacggt gacagaacgc taagattatt cttttatatt
     3721 ctggcttgtt tctgctcacc gtaattaaga cgctctctcc gtttggagga gtgaagtgag
     3781 ttccagagat tttctctggc aaacatccag gagcaaagct aatggcttta aatcttcaag
     3841 acaaacaagc gattgttgct gaagtcagcg aagtagccaa aggcgcgctg tctgcagtag
     3901 ttgcggattc ccgtggcgta actgtagata aaatgactga actgcgtaaa gcaggtcgcg
     3961 aagctggcgt atacatgcgt gttgttcgta acaccctgct gcgccgtgct gttgaaggta
     4021 ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac cctgattgca tactctatgg
     4081 aacacccggg cgctgctgct cgtctgttca aagagttcgc gaaagcgaat gcaaaatttg
     4141 aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc gtctcagatc gaccgcctgg
     4201 caactctgcc gacctacgaa gaagcaattg cacgcctgat ggcaaccatg aaagaagctt
     4261 cggctggcaa actggttcgt actctggctg ctgtacgcga tgcgaaagaa gctgcttaat
     4321 cgcagttatc tttttaacgc attcgcttac gtataaactt attctgatat tcaggaacaa
     4381 tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt tgcagctatg tctgtaatgg
     4441 acgttgtaga actgatctct gcaatggaag aaaaattcgg tgtttccgct gctgctgctg
     4501 tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa aactgaattc gacgtaattc
     4561 tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc agtacgtggc gcaactggcc
     4621 tgggtctgaa agaagctaaa gacctggtag aatctgcacc ggctgctctg aaagaaggcg
     4681 tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga agaagctggc gctgaagttg
     4741 aagttaaata agccaaccct tccggttgca gcctgagaaa tcaggctgat ggctggtgac
     4801 tttttagtca ccagcctttt tgcgctgtaa ggcgccagta gcgtttcaca ctgtttgact
     4861 actgctgtgc ctttcaatgc ttgtttctat cgacgactta atatactgcg acaggacgtc
     4921 cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata gcaacaggca ttgcggaaag
     4981 tgttccattt tccggtcaac aaaatagtgt tgcacaaact gtccgctcaa tggacagatg
     5041 ggtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga gaaaaaacgt
     5101 attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct cctttctatc
     5161 cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta tggtctggaa
     5221 gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga gctgcaatac
     5281 gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat ccgtggcgtg
     5341 acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg cgaagcgccg
     5401 gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat tccgctcatg
     5461 acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc ccagctgcac
     5521 cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc gggtaaagtg
     5581 ctgtataacg cgcgtatcat cccttaccgt ggttcctggc tggacttcga attcgatccg
     5641 aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac catcattctg
     5701 cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa agttatcttt
     5761 gaaatccgtg ataacaagct gcagatggaa ctggtgccgg aacgcctgcg tggtgaaacc
     5821 gcatcttttg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg ccgtatcact
     5881 gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt cccggttgag
     5941 tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg cgagctgatc
     6001 tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca gtctggtcac
     6061 aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat ctctgaaacc
     6121 ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta ccgcatgatg
     6181 cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa cctgttcttc
     6241 tccgaagacc gttatgactt gtctgcggtt ggtcgtatga agttcaaccg ttctctgctg
     6301 cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga tgttatgaaa
     6361 aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca cctcggcaac
     6421 cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg cctggtacgt
     6481 gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct gatgcctcag
     6541 gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg ttccagccag
     6601 ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa acgtcgtatc
     6661 tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag gcttcgaagt tcgagacgta
     6721 cacccgactc actacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc gaacatcggt
     6781 ctgatcaact ctctgtccgt gtacgcacag actaacgaat acggcttcct tgagactccg
     6841 tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc tgctatcgaa
     6901 gaaggcaact acgttatcgc ccaggcgaac tccaacctgg atgaagaagg ccacttcgta
     6961 gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg cgaccaggtt
     7021 gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct gatcccgttc
     7081 ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg tcaggccgtt
     7141 ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc tgttgccgtt
     7201 gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt ggatgcttcc
     7261 cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg tatcgacatc
     7321 tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca gatgccgtgt
     7381 gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc gtccaccgac
     7441 ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg gaatggttac
     7501 aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg tttcaccacc
     7561 atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctggggcc ggaagagatc
     7621 accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga atccggtatc
     7681 gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt aacgccgaaa
     7741 ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg tgagaaagcc
     7801 tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac ggttatcgac
     7861 gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga aatcgaagaa
     7921 atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct cgaagcgggt
     7981 ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga gaagctcgac
     8041 aaactgccgc gcgatcgctg gctggagctg ggcctgacag acgaagagaa acaaaatcag
     8101 ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa gaaactcgaa
     8161 gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct gaagattgtt
     8221 aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc aggtcgtcac
     8281 ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta cgatgaaaac
     8341 ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat gaacatcggt
     8401 cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa gatcaacgcc
     8461 atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg tgcgtacgat
     8521 ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga agaagttatg
     8581 cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt cgacggtgcg
     8641 aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc cggtcagatc
     8701 cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt tggttacatg
     8761 tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc caccggttct
     8821 tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg tcagcgtttc
     8881 ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct gcaggaaatg
     8941 ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa catcgtggac
     9001 ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt gaaagagatt
     9061 cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa acaggtcact
     9121 gctgtcgggt taaaacccgg cagcggattg tgctaactcc gacgggagca aatccgtgaa
     9181 agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg cgatcaaaat
     9241 tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta aaaagccgga
     9301 aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg cccgtatctt
     9361 tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga aacaccgtgg
     9421 cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc gtgagcgtat
     9481 gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat cgctgccgtc
     9541 ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac tgtactttga
     9601 atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga tcctgactga
     9661 agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga agatgggtgc
     9721 ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg aacagctgcg
     9781 tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca agcgtatcaa
     9841 actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc tgaccgttct
     9901 gccggtactg ccgccagatc tgcgtccgct ggttccgctg gatggtggtc gtttcgcgac
     9961 ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc tgaaacgtct
    10021 gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc tgcaggaagc
    10081 ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt ctaacaagcg
    10141 tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc gtcagaacct
    10201 gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc catacctgcg
    10261 tctgcatcag tgcggtctgc cgaagaaaat ggcactggag ctgttcaaac cgttcatcta
    10321 cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga aaatggttga
    10381 gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac acccggtact
    10441 gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac cggtactgat
    10501 cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg ccgacttcga
    10561 tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg aagcgcgtgc
    10621 gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa tcatcgttcc
    10681 gtctcaggac gttgtactgg gtctgtacta catgacccgt gactgtgtta acgccaaagg
    10741 cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct ctggtctggc
    10801 ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg ctaacggtga
    10861 attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc tgtggatgat
    10921 tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa aagcaatctc
    10981 caaaatgctg aatacctgct accgcattct cggtctgaaa ccgaccgtta tttttgcgga
    11041 ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg ttggtatcga
    11101 tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag cagaagttgc
    11161 tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct acaacaaagt
    11221 tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg ataacctgca
    11281 aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct tcaacagcat
    11341 ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc agcttgctgg
    11401 tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa tcaccgcgaa
    11461 cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg ctcgtaaagg
    11521 tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc gtctggttga
    11581 cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag gtatcatgat
    11641 gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg tactgggtcg
    11701 tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc cgcgcaacac
    11761 gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg cggtaaaagt
    11821 acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct acggtcgtga
    11881 cctggcgcgt ggccacatca tcaacaaagg tgaagcaatc ggtgttatcg cggcacagtc
    11941 catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg gtgcggcatc
    12001 tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa ggtagcatca agctcagcaa
    12061 cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta atactgaact
    12121 gaaactgatc gacgaattcg gtcgtaccaa agaaagctac aaagtacctt acggtgcggt
    12181 actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa actgggaccc
    12241 gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg acatgatcga
    12301 cggtcagacc attactcgtc agacagacga attgaccggt ctgtcttcgc tggtggttct
    12361 ggattccgca gaacgtacag caggtggtaa agatctgcgt ccggcactga aaatcgttga
    12421 tgctcagggt aacgacgttc tgatcccagg taccgatatg cctgcgcagt acttcctgcc
    12481 gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg acaccctggc
    12541 gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc cgcgcgttgc
    12601 ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc ctggctgaaa tcagcggtat
    12661 cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc cggtagacgg
    12721 tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt tcgaaggtga
    12781 acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg acattctgcg
    12841 tctgcgtggt gttcatgctg tgactcgtta catcgttaac gaagtacagg acgtataccg
    12901 tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc agatgctgcg
    12961 taaagctacc atcgttaacg cgggcagctc cgacttcctg gaaggcgaac aggttgaata
    13021 ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg gtgcaactta
    13081 ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct tcatctccgc
    13141 ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg gcaaacgcga
    13201 cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg caggtaccgg
    13261 ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc cggctgcacc
    13321 gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg caggtctggg
    13381 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
    13441 ttttttatgg ggggagttta gggaaagagc atttgtcaga atatttaagg aatttctgaa
    13501 tactcataat caatgtagag atgactaata tcctgaaact gactgaacta attgagtcaa
    13561 actcggcaag gattcgatac tattcctgtg taactttctt aaggaacgag aatgaaacag
    13621 gaagtggaaa agtggcgacc ttttggacat ccggatggtg atattcgtga tttatcattt
    13681 cttgatgctc atcaggctgt ctacgttcag catcatgagg gcaaagagcc tttagagtat
    13741 cgcttttggg ttacctactc tcttcactgc ttcacaaaag attatgaaca tcagacgaac
    13801 gaagaaaaac aatcgttaat gtaccacgcg cctaaagaat ctcgtccctt ctgccagcac
    13861 cgttataact tagcgcgcac acacttaaaa agaactattt tggcgctgcc agaaagcaac
    13921 gttattcatg ccgggtatgg tagctatgcc gtgattgagg tggacttaga cggaggagat
    13981 aaggcatttt actttgttgc gttcagggct ttcagggaaa agaaaaaact ccgtttgcat
    14041 gtaactagcg cttatcccat ttctgaaaaa cagaaaggta aatcagtgaa atttttcacc
    14101 attgcctaca acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    14161 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    14221 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    14281 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    14341 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    14401 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    14461 gctgacggca ctaccataaa aaaacagaac gctttcaaca accttgcgtt tttcatcttc
    14521 cgtagcggct cggaataaat ttacgttctt acattccgtc tcatagtctt tgcgaggccc
    14581 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14641 cagcaaccgc ttccggtctg cgatcgtcgt gcggtgagaa ctgttccaac tcggggtgat
    14701 tatcggcata gccacctggc tgcgttttcg agaaggcgct gacgttattg atcgccagcg
    14761 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
    14821 gtgcaagcag tcggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14881 caggctcaat gccgccagta cacgggcgca ggcgcggaaa ggagacagag taacggcttt
    14941 gccagtaatg ctgttgcagc catagcaaat gttcggcaac catatagcag tcaacgcgcc
    15001 agttgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacgcccca
    15061 gccgatccgg cgtttccagc cgccagaaga agtcctgttt ttttcctttc agatgatggc
    15121 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagct
    15181 gctttaactc ggcgtattcc gtctccgcca gcggttgcac ttccatttgt agtgaagaga
    15241 actgttcacg cagggcgggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    15301 gttcaccagt gactaatagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc
    15361 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg attactcatg gaaaatccgc
    15421 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    15481 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    15541 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgaattgc gaggcattta
    15601 gcgcccgctc tacgtcaaca gccgttttgc cgttgatacg caggcggatg tcatcccagt
    15661 ccagttgtcg ccagcgatcg ctgaaggttt tcatgccgat gcctccagaa atccagtcag
    15721 cgggctggtg gcatgagcaa aatgactgcg gctgcccggt ccggactgac gtgccagcag
    15781 gcctgcttct accgccagac gaaatgcctt cgccatgttg acgggatcgt ccgctacggc
    15841 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agggcctgcg cggcatggct
    15901 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
    15961 cagcatggca cgggtttcca gtccctgatt cgagccaatc ggcgcgccga gcggcatcac
    16021 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    16081 cagcacgaca aatccctgtt gtaccagcat ttcggcggct ttcagagttt cgatgggatc
    16141 gggcaacagc cagcgggcgt cagggtgaat ctctaatttt aaccagtttg tgcctaacgc
    16201 ttcacgtgcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggaagtatt
    16261 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    16321 caagtcgaca cgtttcatcg ccagtgtcac cagctggctg ccggaagcgc ggatcgcctc
    16381 caccatcagt tgtgaagaag cgaatttccc tgtgccggta aacagatgtg aatcaaacgt
    16441 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagc aggatctggt
    16501 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
    16561 ccagagccgc gcccgcttgt cgttggtcga gttgctccag tagttcgtga acagtttgcc
    16621 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16681 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctagactt accatcgaac
    16741 agtcggagtt ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc
    16801 tgcaaagtgc ccatcacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16861 tctggctcct ggttatctgg ccacaggcag cggtaacacc cctgctccca gggcggcgtc
    16921 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16981 accacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    17041 acatcggccc gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    17101 gtcagttgaa tgtcgggatt caactgtgtc agtcgctgtt ggctgacctg cgatttcggg
    17161 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    17221 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt
    17281 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    17341 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    17401 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    17461 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    17521 tgccagactg ataccgccaa tcgccacggt gggataatcc gccagtcgct caacatgccg
    17581 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17641 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17701 atgtgtcgaa acacccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17761 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta
    17821 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17881 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17941 atccaacaga cgttcgatcc actgtacgct gtccaccact gggtacagtc ctaaacgaaa
    18001 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    18061 ccgcctctgg cacggaagtt ctccgacata tccgccattc ccatttcaat agtttgcgtg
    18121 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
    18181 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    18241 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    18301 ttcgacatgg cgttatcgcg aatttgcgcg cccggatgcc ctttcgccag gtcagcggcg
    18361 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt gggcagaccc
    18421 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    18481 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    18541 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18601 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacgtcata ttcccaggca
    18661 attttggtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18721 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18781 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18841 catttcgcca taatcgaacc gccgcgagag acgataccgg tcaggcgttt tgcggtcatc
    18901 ggcacatagc gcagcagtac gcccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18961 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccgttaacc
    19021 ttctccagcg cctggtagat cggcactgta ccgatcggca ccgggctgtt acgcaaaatc
    19081 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    19141 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    19201 ttgccgatat tggcgttaac ttttaccagg aagttacggc caataatcat cggctccgat
    19261 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    19321 aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg
    19381 cgtaaaactt cgctacgtat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    19441 atttccggcg tgatgatgcc ctggcgggcg tagtgcagtt gggtgacacg gcgtcctgct
    19501 ttggcgcgtt ttggtgttag tacgccgcta aaacgcagtt cgtcgaggcc atcatctgcc
    19561 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19621 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc aatctgcgga
    19681 tcaccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19741 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcga
    19801 acgccggggt gcgtgccagt gatataaata cgttttgagt tgggaaaggc ggtgccttcc
    19861 agggtgtcga taaaatgttg ggcgtgggcg cgctgttcgc ggcgggtcag ttttgttgca
    19921 gacatagctc attccaaaag ttaaggacgt ggcttgtcag acgacggatg aagcaagaga
    19981 cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc aggtattagc
    20041 ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca ctccgacaag
    20101 aaattagccc cgcaaatggg gcattgaatg taaattacgc gttaacagca cagaactcaa
    20161 gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc ttccagcaca
    20221 aagcgcgctt ccagcgcctc gccgatgtca gataaaacct gttgaaactc aaggtaatta
    20281 tcatgatcga tagcggtttc caggctggaa tcgtagtaat ccataatctg ttgagtattg
    20341 gcttcaagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta
    20401 tgaagaatgc gctcataaat actgaaatgc ccggcagaca agtaatcgac caggctctga
    20461 caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc aggcttaatg
    20521 ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca gcgatcaacc
    20581 agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag catgattgac
    20641 tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc
    20701 cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt agatcacggc
    20761 tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt gccatatggc
    20821 gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga atggcagggg
    20881 gaacctgttt ggttagtaca acagcagcgg cgtcacgata tggggtcggt acgtcaggtc
    20941 attgatctcg atgttgggct gtttcaactg gccggacgag gcgtacaact ggcggagttt
    21001 taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccgag caaaaccgaa
    21061 tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc cccctgcatt
    21121 attgttgcta tccgtcgcga tgattcgatc ctcctcgccc agcatacccg ccatcgtaac
    21181 ggtgtccata cagtacttgc cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc
    21241 gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta cgtgacttct
    21301 cagccgtggc cgtttcctca gtctttaatg accgcgttta tggcggaata tgacagcggc
    21361 gacatcgtga tcgacccgaa agaattgctc gaggcgaact ggtatcgcta tgacgatttg
    21421 ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac ggtggcgatg
    21481 tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg aacagccaaa
    21541 atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc
    21601 actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa agccacgcgc
    21661 gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact
    21721 ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc tcttttccga tatcctcacc
    21781 gtgccggacg cgatggggtt agggctctat tttgaagccg gagaaggtcc gcgttttacc
    21841 tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc ggaagatgag
    21901 ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg cgaagtgccg
    21961 ctgattggtt tttccggcag cccgtggacg ctggcaacct acatggtgga aggcggcagc
    22021 agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc gctgcacgct
    22081 ctactcgata aactggcgaa aagcgtcact ttgtatctga atgcgcagat taaagccggt
    22141 gctcaggctg tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg cgattatcaa
    22201 cagttctcgc tttattacat gcataaaatt gttgatggtt tactgcgtga aaacgacggt
    22261 cgccgcgtac cggtcacgct gtttaccaaa ggcggcggac agtggctgga agccatggca
    22321 gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga tgcgcgccgc
    22381 cgtgtgggca ataaagtcgc gttgcagggt aatatggatc cgtcgatgct gtacgcgccg
    22441 cctgcccgca ttgaagaaga agtagcgact atacttgcag gtttcggtca cggcgaaggt
    22501 catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca tgctggcgtg
    22561 ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc gctaaggagt gattatggat
    22621 ctcgcgtcat tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg cgaggatcga
    22681 ctcgataaag atccaccgga tctgatcgcc ggagccgatg tcgggtttga gcagggcgga
    22741 gaagtgacgc gagcggcgat ggtgctgctg aaatatccct cgcttgagct ggtggagtat
    22801 aaagttgccc gcatcgccac caccatgcct tacattccag gttttctttc cttccgcgaa
    22861 tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc
    22921 gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg ccagccattt tggtttgatg
    22981 gtggatgtgc caacgatcgg cgtggcgaaa aaacggctct gcggtaaatt cgaaccgctc
    23041 tccagcgaac cgggcgcgct ggccccactg atggataaag gcgagcagct ggcctgggtc
    23101 tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg ggtcagcgtg
    23161 gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc ggagccaacg
    23221 cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag
    23281 ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat gttacaaaac
    23341 ccaattcatc tgcgtctgga gcgcctggaa agctggcagc atgtcacttt catggcttgc
    23401 ttatgcgaac gcatgtaccc caattacgcc atgttctgcc agcaaaccgg ttttggtgat
    23461 gggcaaattt accgtcgtat tctcgatctc atctgggaaa cgctgaccgt taaagacgca
    23521 aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc agccgacgat
    23581 ttcgatctgt acggcgttta tccggcaatc gatgcctgcg tggcgttaag tgaactggtc
    23641 cattcgcgtt tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc
    23701 acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga tgaagagctt
    23761 aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt ccgactttta
    23821 gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag
    23881 gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac gtgatttaac
    23941 gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta catttggggg
    24001 gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg catttccgtc
    24061 gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa caagactcaa
    24121 ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa agctgctctg
    24181 gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt acaactggtt
    24241 ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa cccgcagacc
    24301 ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg caaggcactg
    24361 aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca
    24421 ccccttttgt ctttctggcg tcgatcattg atgctggcag gtgcgctgtt tctcactgcc
    24481 tgtagccaca actcttcact tcctcccttt accgccagtg gatttgctga agaccagggc
    24541 gcggtacgca tctggcgaaa agacagcggc gataatgtgc atctgcttgc cgtgtttagc
    24601 ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga taacctcacg
    24661 ctcatcaata tcaatgttta cagcaaaccg ccggtgaaca ttcgcgcgcg ttttgacgat
    24721 cgcggtgatc tgagctttat gcaacgtgaa tccgatgggg aaaagcagca gctttctaac
    24781 gaccaaatcg atttataccg ttatcgtgct gctcagatcc gccagattag cgatgcctta
    24841 cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggaaca gaccgtgacc
    24901 acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc gcatatcgag
    24961 cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt
    25021 tcgcaattac tgttagtggc aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg
    25081 ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg ccgtagccca
    25141 tgcccattcc ggctccgcgc ggaatacccg cttcagccat cgcgatatcc cgtttcaccc
    25201 gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg ttaattttgc
    25261 tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttc gtcaccagtt
    25321 gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc cacgctgtct
    25381 gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accgtgtccg ccgtgagcaa
    25441 atgcagatgt cgatcccatc gccattgctg aaagcgccat cattaccagg gcaattttcg
    25501 tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg catcttccgt
    25561 gccaacgatg aaacgctgat atgacgggta atctggcatg ataaacgagt aaaaatgact
    25621 cgcctgctgc gggtagcgag tcatttttac tcattgaaac ttaagccttt gtgttacagc
    25681 gcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg atgagcaggc
    25741 aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat ggttaagcgc
    25801 gatcctcccc gtggtcattg ttgggctggt agggttgttt gcggtgacgg tgattcgcga
    25861 ttatgggcgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca gtgtacttat
    25921 tcgcgctctt gaatccggct cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca
    25981 gcaggcctta ctggaagaaa tggccgggca gcctggagta cgttggtttg cggtcacgga
    26041 tgaacaagga acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac agctttattc
    26101 cccgcaggaa atgcagcagt tacatcctgg agatgaagaa gcgtggcggc ggatcgatag
    26161 cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt ttgctgctgg
    26221 aatgcaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa ttttcatcgc
    26281 tttcgacgcc agtaatattg tgagtgccga agatcgtgag cagagaaaca ccctgattat
    26341 cctcttcgcc ctggcgacgg tcttgctggc aagcgtgttg tcattcttct ggtatcgccg
    26401 ctatctgcgc tcgcgccagc tgttgcagga tgaaatgaag cgcaaagaga agctggtagc
    26461 tctggggcat cttgcggcag gcgttgccca cgaaatccgt aatccacttt cctcaattaa
    26521 agggctggcg aaatactttg ccgaacgcgc gccagcaggg ggagaagcgc atcaattggc
    26581 gcaggtgatg gcgaaagaag ccgaccgttt aaaccgcgtg gtaagcgagt tgctggaact
    26641 ggttaagcca acgcatctgg ctttgcaggc ggtggatctc aacacgctga ttaaccactc
    26701 attacagctg gtaagccagg atgcaaacag ccgggagatc cagttacgct ttaccgccaa
    26761 cgacacatta ccggaaattc aggccgatcc ggacaggctg actcaggtcc tgttgaatct
    26821 ctatctcaat gctattcagg cgattggtca gcatggcgtg attagcgtga cggtcagcga
    26881 aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg cggcagatca
    26941 gcttgaagcc atcttcactc cgtacttcac caccaaagcc gaaggcaccg gattggggct
    27001 ggcggtcgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg caagccagga
    27061 gggaaaaggc tcaacgttca ccctctggct tccggtcaat attacgcgta aggacccaca
    27121 aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag ccactgcact
    27181 attttgcagg ctttactgcg cggctggggc tataacgtcg cgctggcgaa cagcgggcga
    27241 caggcgctgg agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg
    27301 gcggagatgg acggcatcgc cacgctgaaa gagatcaaag cgttaaaccc ggcaattccg
    27361 gtgctgatta tgactgcgta ctccagcgtc gagacggcgg tagaggcact gaaaactggg
    27421 gcgctggatt atctcatcaa gccgctggat ttcgataacc tacaggcgac gctggaaaag
    27481 gcgctcgcgc atacgcacag tattgatgct gaaacacctg cggtgactgc cagccagttc
    27541 ggtatggtcg gtaaaagctc ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg
    27601 ccatcggaag ccacggtact gatccacggc gattccggca ccggtaaaga gctggtcgcc
    27661 agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct caactgtgcg
    27721 gcactcaacg aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg ggcgtttact
    27781 ggagccgata aacgccggga agggcgcttt gttgaggcgg acggcggcac gctgtttctc
    27841 gatgaaattg gcgatatctc gccgatgatg caggtgcgtc tgctgcgtgc gattcaggag
    27901 cgcgaagttc agcgtgtcgg tagcaaccag actatctcgg ttgatgtccg gctgattgcg
    27961 gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca ggatctctac
    28021 tatcgcctga atgtggtggc gattgaagta ccatcgctgc ggcaacggcg ggaagatatc
    28081 cctctgctgg ctggccattt tctgcagcgc tttgccgagc gtaatcgcaa ggcggtaaaa
    28141 ggttttacgc cccaggcgat ggatctgttg attcattacg actggccggg aaatattcgt
    28201 gagctggaaa acgcggtgga acgggcagtg gtgctgctga ccggggaata tatttccgaa
    28261 cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc tggggcaaag tcaggatatt
    28321 cagccgctgg tggaagtgga aaaagaggtg attctggcgg cactggagaa aacgggcggc
    28381 aacaaaactg aagccgcccg tcagttaggg atcacgcgta aaacgttatt ggcaaaactg
    28441 tcgcgttagt tctgctcgcg ttcgatagca cgccagccga tatctttccg gcagaagcag
    28501 tcgtcccagt ggatatcggt cattaaggca taggcgcgtt tctgcgcttc tgccacggta
    28561 tgacccagcg cggtgacgca cagtacgcgt ccgccgttgg ttaccacctg ctcgtcatcc
    28621 gccagttttg tgcccgcgtg gaacactttg ccgtcagcca cttcttccag cggcaggccg
    28681 tggatcacat cgccggtgcg gtaatcgccc ggatatccac ccgcagccat caccacgccg
    28741 agagaagcgc gttcatccca ctcggacgtt ttctcgtcca gcttgccttc acaggccgcc
    28801 aggcagagct caaccagatc ggacttcatg cgcagcataa tcggctgggt ttccggatcg
    28861 ccaaagcggc agttaaattc aataaccttc ggattgccct gtttgtcgat catcaggccc
    28921 gcatagagaa aaccggtgta ggtgttgcct tccgccgcca tgcctttcac ggttggccag
    28981 ataatacgtt ccatggtgcg ctgatgaacg tcatcggtta ctaccggcgc gggggagtaa
    29041 gcgcccatcc cgccggtgtt tggtccggta tctttatcgc ctacgcgttt gtgatcctgg
    29101 ctggtagcca tcggcagcac atgctcgccg tccaccatca cgataaagct cgcttcttcg
    29161 ccatcgagga actcttcgat aacgatgcga tgacccgcgt cgccaaaagc gttgcccgcc
    29221 agcatatcgt gaacagccgc ttccgcttct tccagcgtca tcgccacgat aacgcctttc
    29281 ccggcagcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg cagatacgcc
    29341 agcgcaggtt ctacctcggt gaagttctgg tattccgccg tagggatctt atggcgggcc
    29401 aggaaatctt tggtaaacgc ttttgagcct tccagttggg ccgcacctgc ggttgggccg
    29461 aagattttca gcccggcggc gcggaaggtc tcgaccacgc ctttcaccag cggcgcttcc
    29521 gggccgacga tggtcagatc aatcttttcg ttttgtgcga aatccaacag cgccgggata
    29581 tcggtcacgc caatagcaac gttttgcagc gcgggttcca gcgcagtgcc tgcattaccc
    29641 ggagcaacaa aaacagtctc aaccagcggc gactgggccg ctttccaggc cagcgcgtgc
    29701 tcgcgcccgc cgttaccaat cactaatact ttcatctatt gctccattaa tggcggaagt
    29761 ggcgcatgtc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca atcacttcgt
    29821 catcacggat ggaaccgcca ggctggatta cgcaggtcac gcccgcagcg gcggcggcat
    29881 caataccgtc gcggaacggg aagaacgcgt cagaagccat cgaggaacct ttcacttcca
    29941 ggccttcatc agcagcttta ataccggcga ttttcgcgga gtacacgcgg ctcatctggc
    30001 ccgcgccaat accgatggtc atattgtttt tggcatagac gatagcattg gatttcacga
    30061 acttcgccac tttccagcag aacagcgcat cgcgcagttc ctgttcggtc ggctgacgtt
    30121 gggtgacgac gcgcagttct tccgcaccga ccatccccag gtcacgatcc tgaaccagca
    30181 gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg ctcgccccac tgaccgcagg
    30241 tcagaacgcg tacgttctgc ttggcggcgg tgattttcag ggcttcttcg ctggcggaag
    30301 gcgcaataat cacttcgaca aactgacgag aaatgatggc ctgtgcggtt tccgcatcca
    30361 gctcgcggtt aaaggcaatg atgccgccga atgcggaggt tgggtcggtt ttgtacgcgc
    30421 gatcgtaagc atcaagaatg gaattgccga tagctacacc gcaagggttg gcgtgcttca
    30481 caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca tcggtatcgg
    30541 cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtagca acggaggctt
    30601 ctttcacatt ctcttctata tagaaggcag cctgctggtg gctgttctcg ccgtaacgca
    30661 tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgacca gcggcttctt
    30721 tgctttcacc gtggtacgcc ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt
    30781 aggcggcagt gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca agcgtcagcg
    30841 atccttcgtt gtcatccatc tctttaataa tggcgtcgta gtcgctgctc ttcaccacga
    30901 ttgcgacatc tttatggttc ttagcggcgg agcgcaccat ggtcgggccg ccgatatcga
    30961 tgttctcaac cgcatcttcc agcgagcaac cttcacgggc cacggtctgg gcgaacggat
    31021 acaggttaac aaccaccata tcgataggct ggatctgatg ttcttccata atggcatcgt
    31081 cctggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc ttcacgcgtc
    31141 catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc agaccttttt
    31201 ctgctaacag acgggcagtg ccccctgtag acagcagctc cacaccgcgt gcggaaagtg
    31261 cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg cggcggactg
    31321 gacgacgttg ttgcatggta aatcccctgg atttgactat tacagagagc gttagctgaa
    31381 tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt cgcccgcatt
    31441 gtaacgaaaa cgtttgcgca acgctcgcga atttttctct ttcaatggtg atcacaattt
    31501 tgacagtggt taccgtggga aaaatacaga aattacattg atgattgtgg ataactctgt
    31561 gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc atttttctgc
    31621 aatttttcta ttgcggcctg cggagaactc cctataatgc gcctccatcg acacggcgga
    31681 tgtgaatcac ttcacacaaa cagccggttc ggttgaagag aaaaatcctg aaattcaggg
    31741 ttgactctga aaga
//
LOCUS       DAPHDX010000044        31592 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPHDX010000044 DAPHDX010000000
VERSION     DAPHDX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.044"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(30..917)
                     /gene="rarD"
                     /locus_tag="RH058_001967"
     CDS             complement(30..917)
                     /gene="rarD"
                     /locus_tag="RH058_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV3137558.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(969..1436)
                     /locus_tag="RH058_001968"
     CDS             complement(969..1436)
                     /locus_tag="RH058_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV3137559.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1601..2470
                     /gene="pldA"
                     /locus_tag="RH058_001969"
     CDS             1601..2470
                     /gene="pldA"
                     /locus_tag="RH058_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV3137560.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2603..4432
                     /gene="recQ"
                     /locus_tag="RH058_001970"
     CDS             2603..4432
                     /gene="recQ"
                     /locus_tag="RH058_001970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV3137561.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4496..5116
                     /gene="rhtC"
                     /locus_tag="RH058_001971"
     CDS             4496..5116
                     /gene="rhtC"
                     /locus_tag="RH058_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV3137562.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5178..5798)
                     /gene="rhtB"
                     /locus_tag="RH058_001972"
     CDS             complement(5178..5798)
                     /gene="rhtB"
                     /locus_tag="RH058_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV3137563.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5909..6931
                     /gene="pldB"
                     /locus_tag="RH058_001973"
     CDS             5909..6931
                     /gene="pldB"
                     /locus_tag="RH058_001973"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV3137564.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6939..7739
                     /gene="yigL"
                     /locus_tag="RH058_001974"
     CDS             6939..7739
                     /gene="yigL"
                     /locus_tag="RH058_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV3137565.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7815..8714
                     /gene="bioP"
                     /locus_tag="RH058_001975"
     CDS             7815..8714
                     /gene="bioP"
                     /locus_tag="RH058_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV3137566.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8602..9555)
                     /gene="metR"
                     /locus_tag="RH058_001976"
     CDS             complement(8602..9555)
                     /gene="metR"
                     /locus_tag="RH058_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV3137567.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9673..11934
                     /gene="metE"
                     /locus_tag="RH058_001977"
     CDS             9673..11934
                     /gene="metE"
                     /locus_tag="RH058_001977"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV3137568.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(11973..12788)
                     /locus_tag="RH058_001978"
     CDS             complement(11973..12788)
                     /locus_tag="RH058_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV3137569.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13050..13811
                     /gene="udp"
                     /locus_tag="RH058_001979"
     CDS             13050..13811
                     /gene="udp"
                     /locus_tag="RH058_001979"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV3137570.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            13951..15378
                     /gene="rmuC"
                     /locus_tag="RH058_001980"
     CDS             13951..15378
                     /gene="rmuC"
                     /locus_tag="RH058_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV3137571.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15473..16228
                     /gene="ubiE"
                     /locus_tag="RH058_001981"
     CDS             15473..16228
                     /gene="ubiE"
                     /locus_tag="RH058_001981"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV3137572.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16242..16847
                     /gene="ubiJ"
                     /locus_tag="RH058_001982"
     CDS             16242..16847
                     /gene="ubiJ"
                     /locus_tag="RH058_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV3137573.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16844..18484
                     /gene="ubiB"
                     /locus_tag="RH058_001983"
     CDS             16844..18484
                     /gene="ubiB"
                     /locus_tag="RH058_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV3137574.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18563..18832
                     /gene="tatA"
                     /locus_tag="RH058_001984"
     CDS             18563..18832
                     /gene="tatA"
                     /locus_tag="RH058_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV3137575.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18836..19351
                     /gene="tatB"
                     /locus_tag="RH058_001985"
     CDS             18836..19351
                     /gene="tatB"
                     /locus_tag="RH058_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV3137576.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19354..20130
                     /gene="tatC"
                     /locus_tag="RH058_001986"
     CDS             19354..20130
                     /gene="tatC"
                     /locus_tag="RH058_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV3137577.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20172..20954
                     /gene="tatD"
                     /locus_tag="RH058_001987"
     CDS             20172..20954
                     /gene="tatD"
                     /locus_tag="RH058_001987"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV3137578.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20951..21439)
                     /gene="rfaH"
                     /locus_tag="RH058_001988"
     CDS             complement(20951..21439)
                     /gene="rfaH"
                     /locus_tag="RH058_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV3137579.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21606..23099
                     /gene="ubiD"
                     /locus_tag="RH058_001989"
     CDS             21606..23099
                     /gene="ubiD"
                     /locus_tag="RH058_001989"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV3137580.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23145..23846
                     /gene="fre"
                     /locus_tag="RH058_001990"
     CDS             23145..23846
                     /gene="fre"
                     /locus_tag="RH058_001990"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV3137581.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24038..25201)
                     /gene="fadA"
                     /locus_tag="RH058_001991"
     CDS             complement(24038..25201)
                     /gene="fadA"
                     /locus_tag="RH058_001991"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV3137582.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25211..27400)
                     /gene="fadB"
                     /locus_tag="RH058_001992"
     CDS             complement(25211..27400)
                     /gene="fadB"
                     /locus_tag="RH058_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV3137583.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27590..28921
                     /gene="pepQ"
                     /locus_tag="RH058_001993"
     CDS             27590..28921
                     /gene="pepQ"
                     /locus_tag="RH058_001993"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV3137584.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28921..29535
                     /locus_tag="RH058_001994"
     CDS             28921..29535
                     /locus_tag="RH058_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV3137585.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29574..31025
                     /gene="trkH"
                     /locus_tag="RH058_001995"
     CDS             29574..31025
                     /gene="trkH"
                     /locus_tag="RH058_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV3137586.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31037..31582
                     /gene="hemG"
                     /locus_tag="RH058_001996"
     CDS             31037..31582
                     /gene="hemG"
                     /locus_tag="RH058_001996"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV3137587.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7303 a         8077 c         8673 g         7539 t
ORIGIN      
        1 ctcattaaac ggcctaccca tgttaattct taactttttc tacgctgcgt ataaatcgca
       61 tccatcacaa aaattgccag cgccacccaa ataaaggcga aagtcaccat cttatcggca
      121 cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac
      181 tggaaaaagc ctaacgttga gagacgcagg cgcgtggcag cggcagtaaa acacaacagc
      241 ggtacggtag tgacaatacc ggcggcgatc agcagtaaat tcagcgacat cgggttttgc
      301 cccatatggc tggttgagct gtcggcaata gcaaacaggt aaatcgccgc cacgggcagc
      361 agccacatgg tttcaattaa catgccggtt tgtgcttcta ccgcaatctt cttgcgcacc
      421 aggccgtaga aggcaaaact aaatgccagc cccagcgcga taataggtag cgaaccaaaa
      481 gtccacagct ggaccaacac gccacatatc gccagaatca ccgccagcca ttgcatccgg
      541 cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg gttaataaag
      601 taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa cagtagccag
      661 ttgccaccaa tcagcacggc agagactgcc agcataaaaa ttttctgtgg cgtctgaatc
      721 agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat aaagaaaaac
      781 gaccagatca cgcgatgcgt caggatttca tcggcgggca cgtagtaaat caacttgaag
      841 tacgctggcg caatacccca aataaaataa gcggcaagag cgagtaatac gccctgccgc
      901 gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt ttacctgctt
      961 ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag ctgttcttca
     1021 ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag gctactggta
     1081 gcagtaaaac gctcgcccct gcctgggcgc agataatcaa cgcgaagatc aatggtcccc
     1141 atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg ggttaaggta
     1201 cttcccacgc acaccagacc ggcggcgaca tccagcgccg acgcaatgac cccgccgtgc
     1261 aaaatgcttt gcgcccagtt gcccaccatc attggctgat ttttaaaggc cagctgtgcg
     1321 aactcttttt cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg catgtgataa
     1381 acaaacatct cacccactaa tttcagggct tgttcagcag tcagtacggc agacatatca
     1441 tccttacact tcattggtta atgaaatgtt gattttatgc ttctttgttg ttggtttcta
     1501 ctttaggaag ggataactaa cggcaatgga gttaagtatg taaaatagcc cgcagaaaaa
     1561 cattcacctt atcaataatt cgttacggag aacacgaccg atgcggactc tgcagggctg
     1621 gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa cggtgaaaga
     1681 ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc aggagcatga
     1741 caatccgttc acgctctatc cttatgacac caactacctc atctacaccc aaaccagcga
     1801 tctgaacaaa gaagcgattg ccagttacga ctgggcggaa aatgcgcgta aggatgaagt
     1861 aaagtttcag ttgagcctgg catttccgct gtggcgtggg attttaggcc cgaactcggt
     1921 gttgggtgcg tcttatacgc aaaaatcctg gtggcaactg tccaatagcg aagagtcttc
     1981 accgtttcgt gaaaccaact acgaaccgca attgttcctc ggttttgcca ccgattaccg
     2041 ttttgcaggt tggacgctgc gcgatgtgga gatggggtat aaccacgact ctaacgggcg
     2101 ttcagacccg acctcccgca gctggaaccg cctttatact cgcctgatgg cagaaaacgg
     2161 taactggctg gtagaagtga agccgtggta tgtggtgggt aatactgacg ataacccgga
     2221 tatcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg gtgatgcggt
     2281 gctcagtgcg aaaggacagt acaactggaa caccggctac ggcggcgcgg agttaggctt
     2341 aagttacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg gctatggcga
     2401 atcgctcatc gactataact tcaaccagac ccgtgtcggt gtaggagtta tgctaaacga
     2461 cttgttttga tgaacggttg agtggttggc aaatctggaa cccagcatcc aggattaccc
     2521 tctcagagac taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt
     2581 atttcaggca atcggggtga atgtggcgca ggcggaagtg ttgaatctgg agtccggagc
     2641 taaacaggtt ttacaagaaa cctttggcta ccaacagttt cgccccggcc aggaagaaat
     2701 tatcgacact gtgctttccg gtcgcgattg cctggtcgtc atgcccaccg gtggcggaaa
     2761 atccctttgc tatcaaattc ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc
     2821 gctgatttcg ttgatgaaag atcaggtgga tcaactgcaa gccaacggcg tggcggcggc
     2881 gtgccttaac tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag gctgccgcac
     2941 cgggcaaatt cgtctgcttt atatcgcccc ggaacgcctg atgctggata actttcttga
     3001 gcatctggcg cactggaatc cggtgttatt agccgttgat gaagcgcact gtatctccca
     3061 atggggccac gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc agcggttccc
     3121 gacgctgccg tttatggcgc tgaccgccac agccgacgac accacgcgcc aggatatcgt
     3181 gcgcctgctg gggctgaacg atccgctgat tcaaatcagc agttttgacc gtccgaatat
     3241 tcgctacatg ctgatggaga agttcaaacc gctcgatcag ttgatgcgct acgtgcagga
     3301 acagcgcggt aagtccggca ttatctactg caacagccgg gcgaaagtag aagacaccgc
     3361 tgcgcgcctg caaagcaagg gtattagcgc ggcggcctat catgccgggc tggaaaataa
     3421 tgtccgcgcc gacgtgcagg agaaattcca gcgcgatgac ctgcaaattg tggtggcgac
     3481 ggtggcgttc ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc actttgatat
     3541 tccgcgcaat atcgaatcct attatcagga aaccggtcgc gccgggcgtg atggcctgcc
     3601 tgcggaagcg atgctgtttt acgatccggc tgatatggcg tggctgcgcc gttgtctgga
     3661 agagaagccg caggggcagt tgcaggatat cgagcgccac aaactcaatg cgatgggcgc
     3721 gtttgccgaa gcgcaaactt gccgtcgtct ggtattgctg aactattttg gcgaagggcg
     3781 tcaggagccg tgcgggaact gcgatatctg cctcgatccg ccgaaacagt acgacggttc
     3841 aaccgatgct cagattgccc tttccaccat tggtcgtgtg aatcagcggt ttgggatggg
     3901 ttatgtggtg gaagtgattc gtggtgctaa taaccagcgt atccgcgact atggtcatga
     3961 caaactgaaa gtctatggca tgggccgtga taaaagccat gaacattggg tgagcgtgat
     4021 ccgccagctg attcacctcg gcctggtgac gcaaaatatt gcccagcatt ctgccctaca
     4081 actgacagag gccgcgcgcc cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc
     4141 gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca actatgatcg
     4201 caaactgttc gccaaattac gcaaactgcg taaatcgatt gccgatgaaa gcaatgtccc
     4261 gccgtacgtg gtgtttaacg acgcaacctt gattgagatg gctgaacaga tgccgatcac
     4321 cgccagcgaa atgctcagcg ttaacggcgt tgggatgcgc aagctggaac gctttggtaa
     4381 accgtttatg gcgctgattc gtgcgcatgt tgatggcgat gacgaagagt agtcagcagc
     4441 ataaaaaagt gccaggatga agactccgta aacgtttccc ccgcgagtca aatgtatgtt
     4501 gatgttattt ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc ccggtcccga
     4561 tttctttttt gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt
     4621 gctgggcatt acctgcggcg taatggtttg ggctgggatt gcgttgcttg gcctgcattt
     4681 gattatcgaa aaaatggcct ggctgcatac gctgattatg gttggcggtg gcctgtatct
     4741 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcag tttctgcacc
     4801 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     4861 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     4921 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     4981 gtggtttacc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     5041 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     5101 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     5161 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     5221 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     5281 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     5341 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     5401 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     5461 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     5521 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     5581 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     5641 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     5701 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     5761 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     5821 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     5881 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     5941 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     6001 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     6061 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     6121 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     6181 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     6241 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     6301 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     6361 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     6421 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     6481 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     6541 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     6601 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     6661 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     6721 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     6781 tgtcgaagga ggacggccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     6841 ggacgcaatg cgctcagtcg cgctccacgc catcgttgat tttttcaaca ggcataactc
     6901 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     6961 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaactc
     7021 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     7081 atgtggggca aattcgcgat aatctggaga ttaagtctta catgattacc tccaatggtg
     7141 cgcgcgttca cgatctggat ggtaatctga tttttgctca taacctggat cgcgacattg
     7201 ccagcgatct gtttggcgta gtcaacgaca atccggacat cattactaac gtttatcgcg
     7261 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     7321 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     7381 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     7441 ggggagatcg cgtcaacgtc agtttctcta ccttaacctg tctggaagtg atggcgggcg
     7501 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     7561 attgtattgc gtttggtgac gggatgaacg acgccgaaat gctgtcgatg gcggggaaag
     7621 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     7681 ttggtactaa tgccgacgac gcggtgccgc attatttgcg taaactctat ttatcgtaat
     7741 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     7801 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     7861 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     7921 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     7981 cagtcggcct gtatatgctg gtgggcgcga tgcagcttgg cgtgatgtat atgctgagtt
     8041 tccgcgctta tctctatctg acggtttccg aactgctgct gttcaccgta ctgacgccgc
     8101 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggatatgctt
     8161 ttagtgcctt gttggcggtg attggtgccg ggattattcg ctatgatcag gtcaccgacc
     8221 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     8281 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     8341 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     8401 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     8461 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggaa
     8521 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
     8581 agccgcactg gccaacgttt attacaggcg cgctggtgat cctggcctca ctgtgggtgc
     8641 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
     8701 cgctgagcga ataaacgctt ccgtaactgg ctgacgctgc tcgccatcgc gcacggcggc
     8761 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc
     8821 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
     8881 aatcaacaat aaggtgttat cgacgctttt tagtgacgga ctgacgcctg ccggctgaag
     8941 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaattaata gcgtctcgct
     9001 ggcgagatct tccggtgtaa tacgcgtttt tgccgccagt ggatggtcag gtgctaacac
     9061 cagacgcact tcatagtcga acatcggcga ataatgcaga ccactgcgcg gcagaatatc
     9121 ggacgtcatt accagatcca gctcgccctg ttgcaacgcg ggctgcgggt caaacgtcac
     9181 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
     9241 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
     9301 ttcattgcag gcttgcagtg cctggctaat ttgcggcagc acctggtttg ccagttgcaa
     9361 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
     9421 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
     9481 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
     9541 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
     9601 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaatacta aaaattccat
     9661 aaggggcata taatgacaat tcttaatcac accctcggtt tccctcgcgt tggcctgcgt
     9721 cgcgagctga aaaaagcgca agaaagttat tgggcgggga actccacgcg tgaagaactg
     9781 ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca agcgggtatc
     9841 gacctgctgc cggtgggcga ttttgcctgg tacgatcatg ttctgaccac cagtctgctg
     9901 ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat cgacaccctg
     9961 ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc
    10021 aaatggttta acaccaacta tcactacatg gtgccggaat tcgttaaagg ccaacagttc
    10081 aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct gggccacaac
    10141 gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt gaaaggtgaa
    10201 cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca agtgctggca
    10261 gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct ggtactggaa
    10321 ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca gggacaggtg
    10381 aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac gattactgcg
    10441 ctgcctgttc agggtctgca tgttgacctc gtacatggta aagatgacgt tgctgaactg
    10501 cacaagcgcc tgccttctga ctggttgctg tctgcgggtc tgatcaatgg tcgtaacgtc
    10561 tggcgcgccg atcttaccga gaaatatgcg caaattaagg acattgtcgg caaacgtgat
    10621 ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag cgtggaaacg
    10681 cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg ccatgaactg
    10741 gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga gtggagcgcc
    10801 ccgattcagg cacgtcgtca ctctacccgc gtacataatc cggcggtaga aaagcgtctg
    10861 gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg tgccgaagcc
    10921 cagcgtgcgc gttttaaact gccagcgtgg ccgaccacca cgattggttc tttcccgcaa
    10981 accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga cgccaataac
    11041 taccgcacgg gcattgcgga acatatcagg caggccattg ttgaacagga acgtttggga
    11101 ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata ctttggcgag
    11161 cacctcgacg ggtttgtctt tacgcaaaac ggttgggtac agagctacgg ttcccgctgc
    11221 gtgaagccac cgattgttat tggtgacgtt agtcgcccgg caccgattac cgtggagtgg
    11281 gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac ggggccggtg
    11341 accatactct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag
    11401 attgcgctgg cgctgcgtga tgaggtggcc gatctggaag cggcagggat tggtatcatc
    11461 cagattgacg aaccggcgct gcgcgaaggt ttaccactgc gtcgtagcga ctgggacgcc
    11521 tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa agatgacaca
    11581 caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc cattgcggcg
    11641 ctggatgcag acgtcatcac catcgaaacc tcgcgttccg acatggagtt gctggagtcg
    11701 tttgaagagt ttgattatcc gaatgaaatc ggtccaggcg tctatgacat tcactcgcca
    11761 aacgtaccga gcgtggaatg gattgaagcc ctgcttaaga aagcggcaaa acgcattccg
    11821 gcagagcgcc tgtgggtcaa cccagactgt ggcctgaaaa cgcgcggctg gccagaaacc
    11881 cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtctggg gtaaatccaa
    11941 accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc accatactgc
    12001 ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg atagctcggg
    12061 cgataatcag cgttgaatgc atgcccggca tccgggtaga cgataatctc tgctttcgca
    12121 ttagcagccc gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat gctgttatcc
    12181 tgaccgccat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc aacaggttgt
    12241 ttcggtgaat tcagcgactt atcgcccgtc agtttgccgt accacgccac tgcggctttt
    12301 agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca gaatccggtg
    12361 atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac atgatcgaga
    12421 tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata
    12481 tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac
    12541 ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc
    12601 tgcactacaa tgaccactgg cagttggcca tcgctttgct ttggtctggc atggtaagca
    12661 ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccggg
    12721 gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg taaatccaga ttgttgtgtt
    12781 gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc
    12841 atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga
    12901 gtgatttatg tgatttgaat cacttttggt gggtaaattt atgcaacgca tttgcgccat
    12961 ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct
    13021 gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc
    13081 tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg
    13141 tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca
    13201 ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg
    13261 gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc
    13321 gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca
    13381 cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg
    13441 tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatctatt ggcgcgacaa
    13501 ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa cgttacgata
    13561 cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg
    13621 gcgtaatgaa ctatgaaatg gaatctgcaa ctctgctgac catgtgtgca agccagggcc
    13681 tgcgtgccgg tatggtggcg ggcgttatcg ttaaccgcac ccagcaagag atcccgaatg
    13741 ctgagacgat gaaacaaacc gaaagccaag cggtgaaaat cgtggtggaa gcggcgcgtc
    13801 gtctgctgta atcctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg
    13861 cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca attctatgtt
    13921 gtgcgggtaa gtaatgaggc tggaggcgtt gtggatttct caatcatggt ttacgcagtt
    13981 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
    14041 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
    14101 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
    14161 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
    14221 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
    14281 ctcagtgagc agtttgaaaa cctcgctaac cgtatttttg agcacagcaa tcgccgggtt
    14341 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
    14401 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
    14461 acccacgaaa ttcgtaatct acagcaactc aacgcgcaaa tggcccagga agcgatcaac
    14521 ctgacgcgcg cgctgaaagg cgataacaaa acccagggga actggggtga agtggtactg
    14581 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    14641 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggtt gccgcaggga
    14701 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta tttcaacgcc
    14761 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    14821 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    14881 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    14941 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    15001 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgcaa cgcccagcaa
    15061 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    15121 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    15181 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    15241 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    15301 cgacttcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    15361 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    15421 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    15481 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    15541 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    15601 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    15661 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    15721 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    15781 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    15841 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    15901 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    15961 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    16021 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    16081 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    16141 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    16201 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    16261 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    16321 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    16381 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    16441 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    16501 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    16561 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    16621 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    16681 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    16741 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    16801 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt
    16861 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    16921 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    16981 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    17041 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    17101 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    17161 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    17221 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    17281 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    17341 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    17401 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    17461 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    17521 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    17581 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    17641 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    17701 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    17761 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    17821 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    17881 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    17941 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    18001 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    18061 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    18121 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgcgct
    18181 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    18241 attggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    18301 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    18361 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    18421 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    18481 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    18541 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    18601 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    18661 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    18721 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    18781 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    18841 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    18901 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    18961 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    19021 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    19081 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    19141 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    19201 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    19261 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    19321 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    19381 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    19441 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    19501 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    19561 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    19621 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    19681 ctgctggttt ccagctcgct gctgttttat atcggcatgg cgttcgccta ctttgtggtc
    19741 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtaca agtatccacc
    19801 gacatcgcca gctatttaag cttcgttatg gcgttgttta tggcgtttgg tgtctccttt
    19861 gaagtgcctg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    19921 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgct gctgacaccg
    19981 ccggatgtct tctcgcaaac gctgttggcg atcccgatgt actgtctgtt tgaaatcggt
    20041 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    20101 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    20161 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    20221 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    20281 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    20341 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    20401 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    20461 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgcccag ctacgcattg
    20521 ccgcagattt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    20581 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    20641 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    20701 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    20761 aattgctgat cgaaactgat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    20821 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    20881 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    20941 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    21001 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    21061 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa ggggttgccg
    21121 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    21181 tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg
    21241 tggtatgaat cacttctgga tcaaattcga caaacaggta gttgggaaac aatggctcac
    21301 tgactgcagt acgttttcca cgcacgattt tttccagggt gatcatcggt gccaggcaat
    21361 tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    21421 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    21481 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    21541 atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgta gattaagagg
    21601 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cttgacgctg cttgaacagc
    21661 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    21721 ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    21781 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    21841 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    21901 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caagtattga
    21961 acatgccgac aaagcggctg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    22021 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    22081 ttacctgggg gctgacagtg acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    22141 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    22201 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    22261 tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt ccggatacac
    22321 tttcagagta tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg gtgaagtgta
    22381 tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac
    22441 aaggtgaaac ggcgccggaa gggccgtatg gcgaccacac cggttactat aacgaagtcg
    22501 atagtttccc ggtatttacc gtgacgcata ttacccagcg tgaagatgcg atttaccact
    22561 ccacctatac tggtcgtccg ccggatgaac ccgcggtgct gggtgtcgca ctgaacgaag
    22621 tgtttgtgcc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    22681 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    22741 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    22801 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    22861 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    22921 attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc acgaataaat
    22981 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg
    23041 cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    23101 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    23161 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    23221 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    23281 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    23341 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    23401 aaatcgtggt cgatattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    23461 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    23521 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    23581 tgtatgatct ctgcgagctt gaggcgcttt cgttgaagca tcctggtctg caagtggtgc
    23641 cggtggttga acaaccggaa gcgggctggc gtgggcgtac tggcaccgtg ttaacggcgg
    23701 tattgcagga tcacggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    23761 tggcgaaaat tgcccgcgat ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    23821 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    23881 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcattcggca
    23941 cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc cggatcggta
    24001 gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca aacaccgtcg
    24061 caataccctg acccagaccg atacacatcg tcgccagacc aaactggacg tctttgcgct
    24121 ccatcagatt tagcagcgtg gtgctgatac gcgcaccgga acagcccagc ggatgaccca
    24181 gcgcgatcgc gccaccgttg aggttgatct tctcgtcaat ctgctccatt agccccagat
    24241 ctttaataca tggcaggatc tgcgcggcaa aggcttcatt catttcaaac acgccgatat
    24301 cactgacaga aagccccgct tttttcagcg ccagcttcga ggccggaacc gggccgtaac
    24361 ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacgcgggcg cgcggcttaa
    24421 gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg ccatcggaaa
    24481 gcgcagaaga tgtacccgcc gttaccgtac cgcttactgg atcaaacgcc ggacgcagcg
    24541 tggctagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta aactgcttca
    24601 ggacgccgtc ggcatcgtga ccaccggtcg ggataatttc atttttaaat gcacccgact
    24661 gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc
    24721 tgataccgtg catacgcgcc agcatttctg ccgttaagcc catcatgccc gccgctttgg
    24781 cgacattgcg gctcaggccg gggtgaaaat caacgccgtg actcatcggc acatggccca
    24841 tatgctccac gccgccaacc agacatgcct gcgcatcgcc ggtcataatc atccgtgctg
    24901 cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc gccgggacag
    24961 agtgtggtac ttctgccagc agcgccgcgt tacgggcgat gttaaaaccc tgctccagcg
    25021 tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc gccgggttac
    25081 gcgccagcag gctacgcatt aaatgagcgg agagatcttc tgcacgcacg ttacgaaaag
    25141 caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca acctgttcca
    25201 ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca accggaggat
    25261 agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttca tacagcgggc
    25321 cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg gtgtccagcc
    25381 agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc gccatatccg
    25441 cttccgccgg agtggcgata atgctttctt ccagacagcg caccacttcg ttgaccatcg
    25501 ggatcatcat gcgggcgata atctcctctt cgctgaaatc gcgcttcggc tggctcactt
    25561 ctgccagcag gtcgtcaacg acggcgtctt cttctttctt cggcttacct ttgctgtctt
    25621 ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca tcaaacagcg
    25681 cgtcgatggt atcgcgataa tctttctgca tccgctgcgg gaagcctgct gccatgacag
    25741 cctgggcatg atgcgcggtg tcaatgccca cgacatccag cagatatgcc ggacccatcg
    25801 gccagccaaa ctgtttttcc attactttgt cgatcttgcg gaaatccgcg ccgtcgcgca
    25861 gcagctggct gaaaccggcg aaatacggga acagcacgcg gttgacgaag aagccggggc
    25921 agtcgttaac cacaatcggc gttttgccca tcttgctcgc ccaggcgacg actttcgcga
    25981 tggtttcgtc ggagcttttc tcgccgcgaa ttatttctac caacggcatt cggtggaccg
    26041 ggttaaagaa gtgcatcccg cagaagtttt ctggacgttc cagcgcgttg gccagttcgc
    26101 tgataggaat ggttgaagtg ttagacgcca gcacggtatt cgggcgtact ttctgctcgg
    26161 tttctgccag tacggctttt ttcacttttg ggttttcgac aaccgcttct accacaacat
    26221 ccacgcggtc aaacccggcg tagtcgagcg ttgggtggat tgtggagatc acgccagcca
    26281 gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc gcggcttcgg
    26341 tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc acgcctttcc
    26401 acgcagactg gtaagcgatg ccgccgccca taatgcctgc acccagcacc gcggcctgtt
    26461 tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat tgatcgttaa
    26521 ggaaaatgcc gaccagtgcg cgggcttcgt tggtatgcgc cagcgggaca aaacttttgt
    26581 tttccaggtt taaggcttct tcacgaccaa aacgggccgc agcttcaatg gtttttactg
    26641 cggtgatggg ggccggataa tgtttccccg ctgtttgtgc gaccatccct ttagcgatgg
    26701 tgaagctcat ggtggcttca atcttgctca gttttagtgg ttccagcttc ggctgacgtt
    26761 ttgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aaccgccttt gcgccttcaa
    26821 ccagtttttc tgctttgact acgccatcca ccagaccgat tttcagcgcc tgatccgcgc
    26881 cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc atacgtggca
    26941 tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc agaccgatgc
    27001 gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag ccaccgccca
    27061 gcgcatagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg cgattaaaca
    27121 cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg aacagggaca
    27181 aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc agcagcagcc
    27241 cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg gcgacggtcg
    27301 cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca atgccatctt
    27361 ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca
    27421 aggtgatctg gtcgtaccag atgcatcgaa gtgtgtattt tgtgttaaaa atatgcaaat
    27481 aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc tggatgaacg
    27541 atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact
    27601 ggcctcactc tataaaaatc atatagctac cttacaggaa cggactcgcg atgcgctggc
    27661 gcgcttcaag ctggatgcgt tacttattca ctccggcgaa ctgttcaacg tttttctcga
    27721 cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca
    27781 ggtgccaaac tgctggctgc tggtggatgg cgtgaacaag ccgaaactgt ggttctatct
    27841 gccggttgat tactggcaca acgtcgaacc gctgccgacc tctttctgga ctgaagacgt
    27901 ggaagtgatc gcgctgccga aagccgatgg cattggtagc ctgctgcctg ctgcgcgcgg
    27961 gaatatcggt tatatcggtc cggtgccgga acgtgcgctg caactgggta ttgaggccag
    28021 caatatcaac ccgaaagggg tgatcgacta cctgcattac taccgctcct tcaaaaccga
    28081 gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc atcgcgcggc
    28141 agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacattgcct atctgaccgc
    28201 gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctca acgaacacgc
    28261 tgcggtgttg cattacacca aactggacca ccaggcaccg gaagagatgc gcagcttcct
    28321 gctggatgcc ggggccgaat ataacggcta tgcggctgac ctgacccgta cctggtcggc
    28381 aaaaagtgac aacgactacg cacagctggt gaaagacgta aatgatgaac aactggcgct
    28441 gatcgctacc atgaaagcag gcgtcagcta tgtggattac cacatccagt tccatcagcg
    28501 catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag aagcgatggt
    28561 cgaaaacgat ctcaccggac cgtttatgcc gcacggtatt ggccatccgc tgggcctgca
    28621 ggtgcatgac gtagccggtt ttatgcagga tgatagcggt acgcacctcg cggcaccggc
    28681 aaaatatccg tacctgcgct gcacccgtat tctccagccg ggcatggtgt taaccatcga
    28741 accgggtatc tacttcattg aatcgctgct ggcaccgtgg cgtgaagggc agttcagcaa
    28801 gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc gtatcgaaga
    28861 caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga aactggcgtg
    28921 atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat caaaaaaagc
    28981 cgcttcataa cgatgttggc gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa
    29041 tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg
    29101 ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcggg aacggcaggt
    29161 aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc tgtggtagtg
    29221 cgctactacg gcggcatatt gcttggcacc ggtgggttag tgaaagcgta tggcggcggc
    29281 gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac cgaatatact
    29341 ttgcaatgtg aatatagtca gttaaccggc attgaagcgt tgctggggca gtgtgacggc
    29401 aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct tccggcggcg
    29461 aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt gcaattgtta
    29521 gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca gagatgcatt
    29581 ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg accatgatta
    29641 tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt acccagacct
    29701 tttttgtcgc cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa gagaagggcg
    29761 aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ttggaccgtg ctgggtagcg
    29821 tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc gatgcgtttt
    29881 ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg ctggattcgc
    29941 tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg atggggatca
    30001 tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg
    30061 cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg gaaacggcga
    30121 aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg
    30181 ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct attggcggct
    30241 tctcgacaca tgatgccagt atcggttatt tcgacagccc gactattaac actatcattg
    30301 ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg ttaagtgggc
    30361 gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc gtacagttta
    30421 cgctggtggt tatttgtaca ctcgtactgt ggtttcataa tgtctacagt tcggcgctga
    30481 tgacaattaa ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc gggtttacga
    30541 ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt tcagctttta
    30601 tcggcggttg cgccgggtca acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt
    30661 ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta
    30721 agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga tttttctccg
    30781 cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc gtggatgact
    30841 tttctgcctt tgcctcagtt gttgcgacat tgaataacct ggggccaggg cttggcgtgg
    30901 ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt gccaacatgc
    30961 tgtttggtcg tctcgaggtc tttacattgc tggtgctctt taccccgact ttctggcgtg
    31021 aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg gacaaacgcg
    31081 cgagattgcc tcctacctgg cttcggaact gaaagaactg gggatccaga cggatgtcgc
    31141 caatgtgcac cgcattgaag aaccacaatg ggaaaattat gaccgtgtgg tcattggtgc
    31201 ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa aacatgcgac
    31261 gcgactgaat tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga
    31321 gaagcgtact ccacagacca acagctacgc gcggaagttt ctgatgaact cgcaatggcg
    31381 tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc gctggtacga
    31441 ccgttttatg atcaagctga ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga
    31501 agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg cccatttaac
    31561 cgacaaaccg acgctgaaat aagcacaaag aa
//
LOCUS       DAPHDX010000045        31270 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPHDX010000045 DAPHDX010000000
VERSION     DAPHDX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.045"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(129..392)
                     /gene="cedA"
                     /locus_tag="RH058_001997"
     CDS             complement(129..392)
                     /gene="cedA"
                     /locus_tag="RH058_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV3137588.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            575..2836
                     /gene="katE"
                     /locus_tag="RH058_001998"
     CDS             575..2836
                     /gene="katE"
                     /locus_tag="RH058_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV3137589.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGIHR
                     MGIDTNPANYEPNSINDNWPRETLPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2883..3641)
                     /gene="chbG"
                     /locus_tag="RH058_001999"
     CDS             complement(2883..3641)
                     /gene="chbG"
                     /locus_tag="RH058_001999"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV3137590.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(3654..5006)
                     /gene="celF"
                     /locus_tag="RH058_002000"
                     /gene_synonym="chbF"
     CDS             complement(3654..5006)
                     /gene="celF"
                     /locus_tag="RH058_002000"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV3137591.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5111..5953)
                     /gene="chbR"
                     /locus_tag="RH058_002001"
     CDS             complement(5111..5953)
                     /gene="chbR"
                     /locus_tag="RH058_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV3137592.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5961..6311)
                     /gene="chbA"
                     /locus_tag="RH058_002002"
     CDS             complement(5961..6311)
                     /gene="chbA"
                     /locus_tag="RH058_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV3137593.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6362..7720)
                     /gene="chbC"
                     /locus_tag="RH058_002003"
     CDS             complement(6362..7720)
                     /gene="chbC"
                     /locus_tag="RH058_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV3137594.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7805..8125)
                     /gene="chbB"
                     /locus_tag="RH058_002004"
     CDS             complement(7805..8125)
                     /gene="chbB"
                     /locus_tag="RH058_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV3137595.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8424..8762)
                     /gene="osmE"
                     /locus_tag="RH058_002005"
     CDS             complement(8424..8762)
                     /gene="osmE"
                     /locus_tag="RH058_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV3137596.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8964..9791
                     /gene="nadE"
                     /locus_tag="RH058_002006"
     CDS             8964..9791
                     /gene="nadE"
                     /locus_tag="RH058_002006"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV3137597.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10021..10908
                     /gene="cho"
                     /locus_tag="RH058_002007"
     CDS             10021..10908
                     /gene="cho"
                     /locus_tag="RH058_002007"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV3137598.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10868..11443)
                     /gene="ves"
                     /locus_tag="RH058_002008"
     CDS             complement(10868..11443)
                     /gene="ves"
                     /locus_tag="RH058_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV3137599.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11646..12131)
                     /gene="spy"
                     /locus_tag="RH058_002009"
     CDS             complement(11646..12131)
                     /gene="spy"
                     /locus_tag="RH058_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV3137600.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12461..13429)
                     /gene="astE"
                     /locus_tag="RH058_002010"
     CDS             complement(12461..13429)
                     /gene="astE"
                     /locus_tag="RH058_002010"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV3137601.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13422..14765)
                     /gene="astB"
                     /locus_tag="RH058_002011"
     CDS             complement(13422..14765)
                     /gene="astB"
                     /locus_tag="RH058_002011"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV3137602.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14762..16240)
                     /gene="astD"
                     /locus_tag="RH058_002012"
     CDS             complement(14762..16240)
                     /gene="astD"
                     /locus_tag="RH058_002012"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV3137603.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16237..17271)
                     /gene="astA"
                     /locus_tag="RH058_002013"
     CDS             complement(16237..17271)
                     /gene="astA"
                     /locus_tag="RH058_002013"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV3137604.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17268..18488)
                     /gene="astC"
                     /locus_tag="RH058_002014"
     CDS             complement(17268..18488)
                     /gene="astC"
                     /locus_tag="RH058_002014"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV3137605.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKASVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            18934..19740
                     /gene="xthA"
                     /locus_tag="RH058_002015"
     CDS             18934..19740
                     /gene="xthA"
                     /locus_tag="RH058_002015"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV3137606.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19907..20617
                     /locus_tag="RH058_002016"
     CDS             19907..20617
                     /locus_tag="RH058_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV3137607.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20622..21299
                     /gene="ydjY"
                     /locus_tag="RH058_002017"
                     /pseudo
     CDS             20622..21299
                     /gene="ydjY"
                     /locus_tag="RH058_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            21314..22021
                     /locus_tag="RH058_002018"
     CDS             21314..22021
                     /locus_tag="RH058_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV3137608.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22021..22569
                     /locus_tag="RH058_002019"
     CDS             22021..22569
                     /locus_tag="RH058_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV3137609.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22579..23745
                     /locus_tag="RH058_002020"
     CDS             22579..23745
                     /locus_tag="RH058_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3137610.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23718..25253
                     /locus_tag="RH058_002021"
     CDS             23718..25253
                     /locus_tag="RH058_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDV3137611.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25253..25906
                     /locus_tag="RH058_002022"
     CDS             25253..25906
                     /locus_tag="RH058_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV3137612.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25973..27280
                     /gene="ynjE"
                     /locus_tag="RH058_002023"
     CDS             25973..27280
                     /gene="ynjE"
                     /locus_tag="RH058_002023"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV3137613.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTPDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27289..27909)
                     /locus_tag="RH058_002024"
     CDS             complement(27289..27909)
                     /locus_tag="RH058_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV3137614.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            27996..28403
                     /gene="nudG"
                     /locus_tag="RH058_002025"
     CDS             27996..28403
                     /gene="nudG"
                     /locus_tag="RH058_002025"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV3137615.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28426..28641)
                     /locus_tag="RH058_002026"
     CDS             complement(28426..28641)
                     /locus_tag="RH058_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HDV3137616.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQC"
     gene            28877..30220
                     /gene="gdhA"
                     /locus_tag="RH058_002027"
     CDS             28877..30220
                     /gene="gdhA"
                     /locus_tag="RH058_002027"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV3137617.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30337..>31270)
                     /locus_tag="RH058_002028"
     CDS             complement(30337..>31270)
                     /locus_tag="RH058_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3137618.1"
                     /translation="MLLCKWGFYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLL
                     IMMGLIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWP
                     RKPEPGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANT
                     FKANMEEAPEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMISISSTA
                     FQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELE
                     ACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         7715 a         8243 c         7714 g         7598 t
ORIGIN      
        1 gatcacttat ttatagcaat agatcgtcaa aggcagcttt ttgttactgg tggtttgaat
       61 gaatgtagca atgaaataca gaatttcagg tcatgtaact cccggcaaaa ccgggaggta
      121 cgtaatcttt actcagtcac ttccccttcc tggcggatct gatttgccca acgctgggcg
      181 gattcaggca cagtaaacgc cggtgagcgc agaaatgact ctcccatcag tacgaacgca
      241 acatatttgc cacgcagcat ccagacatca cgaaacgaat ccatctttat cgcatgttct
      301 ggcggcgcgg gttccgtgcg tgggacatag ctaataatct ggcggttttg ctggcggagc
      361 ggtttcttca ttactggctt cactaaacgc atattaaaaa tcagaaaaac tgtagtttag
      421 ccgatttagc ccctgtacgt cccactttgc gtgtatttca taacaccgtt tccagaatag
      481 tctccgaagc gggatctggc tgatggtcta tagttagagg gttttttgac caaaacagcg
      541 gccctttcag taataaatta aggagacgag ttcaatgtcg caacataacg aaaagaaccc
      601 acatcagcac cagtcaccac tacacgattc cagcgaagcg aaaccgggga tggactcact
      661 ggcacctgag gacggatctc atcgtccagc ggctgaacca acaccgccag gtgcacaacc
      721 taccgcccca gggagcctga aagcccctga tacgcgtaac gaaaaactta attctctgga
      781 agacgtacgc aaaggcagtg aaaattatgc gctgaccact aatcagggcg tgcgcatcgc
      841 cgatgatcaa aactcactgc gtgccggtag ccgtggtcca acgctgctgg aagattttat
      901 tctgcgcgag aaaatcaccc actttgacca tgagcgcatc ccggaacgta ttgttcatgc
      961 acgcggatca gccgctcacg gttatttcca gccatataaa agcttaagcg atatcaccaa
     1021 agcggatttc ctctcagatc cgaacaaaat caccccagta tttgtacgtt tctctaccgt
     1081 tcagggtggt gctggctctg ccgataccgt gcgtgatatc cgtggttttg ccaccaagtt
     1141 ctatactgaa gagggtattt ttgacctcgt tggcaataac acgccaatct tctttatcca
     1201 ggatgcgcat aaatttcccg attttgttca tgcggtaaaa ccagaaccgc actgggcaat
     1261 tccacaaggg caaagcgccc acgatacttt ctgggattat gtttctctgc aacctgaaac
     1321 tctgcacaac gtgatgtggg cgatgtcgga tcgcggcatc ccccgcagtt accgcaccat
     1381 ggaaggcttc ggtattcaca ccttccgcct gattaatgcc gaagggaagg caacgtttgt
     1441 acgtttccac tggaaaccac tggcaggtaa agcctcactc gtttgggatg aagcacaaaa
     1501 actcaccgga cgtgacccgg acttccaccg ccgcgagttg tgggaagcca ttgaagcagg
     1561 cgattttccg gaatacgaac tgggcttcca gttgattcct gaagaagatg aattcaagtt
     1621 cgacttcgat cttctcgatc caaccaaact gatcccggaa gaactggtgc ccgttcagcg
     1681 tgtcggcaaa atggtgctca atcgcaatcc ggataacttc tttgccgaaa acgaacaagc
     1741 agcattccat ccaggtcata ttgttcctgg tctggacttc accaacgatc cgctgttgca
     1801 gggacgtttg ttctcctata ccgatacaca aatcagtcgt cttggtgggc cgaatttcca
     1861 tgagattccg attaaccgtc cgacctgccc ttaccacaac ttccagcgtg atggcataca
     1921 tcgtatgggg atcgacacta acccggcgaa ttacgaaccg aactcgatca acgataactg
     1981 gccgcgcgaa acactgccgg gaccgaaacg cggcggtttt gaatcatatc aggaacgcgt
     2041 ggaaggaaat aaagttcgtg agcgcagtcc atcgtttggc gaatattatt cccatccgcg
     2101 tctgttctgg ctaagtcaga cgccattcga gcagcgccat attgtcgatg gtttcagttt
     2161 tgagttaagc aaagtcgttc gtccgtatat tcgtgagcgc gttgttgacc agctggcgca
     2221 tattgatctc actctggccc aggcggtggc gaaaaatctc ggtatcgaac tgactgacga
     2281 ccagctgaat atcaccccac ctccggacgt caacggtctg aaaaaggatc catccttaag
     2341 tttgtacgcc attcctgacg gtgatgtgaa aggtcgcgtg gtagcgattt tacttaatga
     2401 tgaagtgaga tcggcagacc ttctggccat tctcaaggcc ctgaaggcca aaggcgttca
     2461 tgccaaactg ctctactccc gaatgggtga agtgactgcg gatgacggta cggtgttgcc
     2521 tatagccgct acctttgccg gtgcaccttc gctgacggtc gatgcggtca ttgtcccttg
     2581 cggcaatatc gcggatatcg ctgacaacgg cgatgccaac tactacctga tggaagccta
     2641 caaacacctt aaaccgattg cgctggcggg agacgcgcgc aagtttaaag caacaatcaa
     2701 ggtcgctgac cagggtgaag aagggattgt ggaagctgac agcgccgacg gtagttttat
     2761 ggatgaactg ttaacgctga tggcagcaca ccgcgtgtgg tcacgcattc ctaagattga
     2821 caaaattccg gcgtaaatat ggataaggtc acgtcccctg gggagtgacc ttaccacctt
     2881 acttacacat taagataact acccaaccga taaccacgct cagcaattgc atatttcaac
     2941 gacgctgatg tcagcacttc cagctccgtt aaccgtggga aacagtaagc actctgacgg
     3001 atagtattat cgataaacgc cggatgacac atcacctcca gcgaacgatc accccgatgg
     3061 cttgcgtcat ccagcacttg caggaacagt gcctcgctaa tctcttcacc ataaaacgcg
     3121 ctactgaatc cctgagaact gcgcagatta gccggtaaat caccagcgtt agatagcggc
     3181 tgacggtcga tgcgcaacgc aatcccctct tcagccgcaa acctggcaac aatcgggaaa
     3241 atctgcggga acatatgcac atgatgatgg ctatcaagat gcgtaggttt gcgtccaaat
     3301 agctcaatga aacgcaaata ctgactggca agctcctgag taatttcttc cagcggtaaa
     3361 gcatcttctt ctgccaactg ccagatccat tttcccagca caccatcgcg ggttaacccc
     3421 ggcatagctg tcagtggctt gcccatagta aggacaaagt gcatccctat ggccagactc
     3481 ggttcatcac gactcaactg caccgcatgg tcaatagcct gcccattcac cagcgccgtc
     3541 gttgatgtga caatcccatt gcgacaggcc tcgataatgc cgtagttctg acctttgctt
     3601 aagccaaaat catcggcatt aacaatcagt aagcgttcca taatcagcct cggttaatgt
     3661 gcttttttaa gctctgcgat gcagtcggca aaatttggca gccatttctc gtgcgccaga
     3721 atcatctcgc gtgccagcag ctcagcatcg cgatcggaat gcaccaacgg actaaggttt
     3781 agcgccagta acacatcgtt aaattctccg ctaagtgcgg cgttgctggc agcaatctcg
     3841 aagcctttaa tggtgtgaat cagccccatc actttatcat cgaaatgcgt aatgcgcgga
     3901 tgtggcgtcg cgccatcgcg ccccagctta caggtcattt ctaccgccca gtctgccgga
     3961 atattatcaa tatgcccatg atgcgggata ttaacgtaat gttcagcttg cttgtcgttg
     4021 tagatagcgt tgatcacttc gcacgctgca tcagagtaat aagccccacc gcgctgttcc
     4081 agttctttcg gcttaacttt caactccgga tttttataca gctcaaaaag ttgtttctcg
     4141 actttctgta ctacctgtgc tcgtgcgccg cctttgtagt attcgcccat ttcaatagcc
     4201 agcatctctt tctgcttgaa gtaatacagc agataagaac atggcagcag atttaacgaa
     4261 cgaattaagc cctcactaaa tggcagatcg aaaatatttt ttacagagga cgcttttaac
     4321 tgccctgacg ccacaccatc aagcaattcg gcaaagcgcg acttgccatt taccagcaca
     4381 tccttaatga acaccatatg gttgaggccg aacagatcga tagataaatc atcgctgtct
     4441 ttcagcatca gaacatcgcg aataaacatc ttcatgccga tcggaatatt acacacgccg
     4501 ataaagcgtt taaatccggt atgacgataa acggcttcag tgaccattcc cgccgggtta
     4561 gtgaagttaa tcacccatgc attcggacaa agttcttcga catcttttac gatgtcaaaa
     4621 atcaccggaa tggtacgcag acctttaaac agaccgcccg cgccgttggt ttcctgacca
     4681 agataaccat gacttaatgg aatacgttca tccagttcgc gcgccggtaa ttggcctacg
     4741 cgcagttggg tagtaacgaa atcagcatct ttcaatgctt cgcggcgatc cagcgtttta
     4801 taaagcttca tcgggacgcc agcgttatca atcatccgtt ggcagagttc aaaaatgata
     4861 tccagtttct ctttaccatc ttcgacatcc accagccata attcgctgac cggcaattcg
     4921 tgataacgct taataaatcc ttccagtaac tccggggtat agctgctccc gccaccaata
     4981 gtgacgactt ttaatttctg gctcataatt tttcccttca gtacagaata ctgatatctg
     5041 gcatatctgc ccccccggac ataaataatc cagcaacagg acagatatgt gaattgtcag
     5101 gtataacgac ttactgatta aattcagtca atttcttacg atagctctta ggcgtaaagg
     5161 acgttaattt cttaaacgtt ttaataaaca aactcgggct actataaccg gcctcaaacg
     5221 caatatccgt cacggaatag ttggtcattt ccagttgttt tttggcaaaa ttaatacgga
     5281 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
     5341 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
     5401 ctttatcatg catcttttct accgtacttt tcagccactg cggtacatca tcaatcacct
     5461 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
     5521 cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca taggtgagaa
     5581 aagcgttatt ggtccggtat acctgcgaag cgacaaagca ataaggcaac aacggcaggt
     5641 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg gcaccaaact
     5701 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
     5761 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
     5821 aatagtcgtg ctgatgcagc ccgctgatac tctcagtttt gttatagata aacacatgga
     5881 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
     5941 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
     6001 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
     6061 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
     6121 gcttcattca atgccatccg tgactgatcc atcatggctt ttgctgcagc aaaatcgccc
     6181 tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg cttgtccgga gttgatgata
     6241 agccccatca ccacttcttc cagctcttca gcttccgttt gcgtatcggg aatgttatcg
     6301 agatccatca tacatcgttc ctctctattc ttaccggcac gattacccgt accggcatcg
     6361 attaaaattt cagggcgtta gcgatatctt cttcgctctc ttctttatca atcgcgtttt
     6421 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgata ccaaggttga
     6481 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     6541 ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg
     6601 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     6661 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
     6721 gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga
     6781 tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg
     6841 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     6901 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     6961 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     7021 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     7081 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     7141 agccgggaat taatgccgag aaggaacgcg atactgaagc aggtacgctg tcgggcagtt
     7201 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     7261 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
     7321 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca gccgccagcg
     7381 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     7441 ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
     7501 caatggttga ggcgtcgagg cgaataccta aggaataaaa aaacgacccc tccccaaagc
     7561 ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc
     7621 gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag
     7681 ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac ccttaaaaac
     7741 cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga aaatttaaaa
     7801 taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac
     7861 catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac
     7921 gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct
     7981 gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt
     8041 tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc gcagaacaaa
     8101 acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag
     8161 acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact
     8221 ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt
     8281 ataaattgtc tctccttgac ctgcggttat gaccctcttc acatttcggg caaataattc
     8341 gcgaagataa ttaagaactc aggccaaaaa aaaccggcgc aatggccggt ttccgttgta
     8401 actcaagctt tcaggcgaag tgattacttc gcagcctgtg gatcagtgtc gtattcagca
     8461 caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac aaagtaggtt
     8521 tctgctttac catcacgttg acccaggatg taggtctggc aagtaccacg agcatggatc
     8581 atgctcactt cagacgaagg cttacctgca atttgtgcaa cctgcgcccg gctcatgcct
     8641 tttttgacgt ctttcaccac aggctgtaca aactggtctt tagtacgatc ataagccgta
     8701 caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat attcttgttc
     8761 atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt gggcgctttt
     8821 tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgatg ttttaaccac
     8881 cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa ggaagttttg
     8941 tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag gcgctgggcg
     9001 caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tgtcgatttt ctgaaaagct
     9061 acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc ggtcaggact
     9121 ccacgcttgc cggaaagctg tgccagatgg cgattaatga gctgcgccag gaaactggca
     9181 acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc gacgaacaag
     9241 attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt aatatcaagg
     9301 gcgcggtatt ggctagcgag caggcattgc gggaagcagg cattgaactg agcgattttg
     9361 tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt gcgggtatga
     9421 ccagcggtgt cgtggtgggc accgatcatg cagcagaagc cattaccgga ttcttcacta
     9481 aatatggtga cggcggtacg gacattaatc cgctgtatcg tctcaacaaa cgtcagggta
     9541 aacagttact ggcggcatta ggttgcccgg aacaccttta taagaaagcg ccaacggccg
     9601 atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg acctatgaca
     9661 atatcgacga ctatctggaa gggaaaaacg tacctgaaca ggtcgccaga acaatagaga
     9721 actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc gatgatttct
     9781 ggaaaaagta ataatttgca cctattggat tgtgcgaaaa agagtaattt gttcacgccg
     9841 gatgcggcgt gaacgcctta ttcgacctac aaaactatgc aaattcaata cattgcagga
     9901 gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca tcaacccgcc
     9961 tcttttttaa tcgcctcccg cctgttacac tggatagata accagcattc ggagtcaaca
    10021 gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat ttatgaatat
    10081 cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt gtatctgttt
    10141 catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaatat ccgcagtcgg
    10201 gtgctttctc atttacgcac cccggatgaa gccgccatgc tacggcaatc ccggcgaatt
    10261 agctggatct gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg attaatcaaa
    10321 gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg tgccctgcaa
    10381 ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc acgagccccc
    10441 aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca gagcatcgct
    10501 gatgaacaaa aactttgtta tggcctgttg ggactggaac cgttaagtcg cggtcgtgca
    10561 tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga gagccatgag
    10621 gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg ttggccttgg
    10681 caaggtgcgg tggcactgaa agaacagcac ccggaaatga ctcaatatca tattattcaa
    10741 aactggctgt ggctgggggc ggttaattcg ctggaagaag cgacaacgtt aattcggaca
    10801 cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct ttccggtaac
    10861 tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt tcactgaaca
    10921 acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac cagcacgcgc
    10981 cctggtcggt agtgagcaat ttatcgccca gttgccacgc cccgttaatc acaaacacca
    11041 ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact ttcgccttac
    11101 agacatccag gcgtgtcatg atgttgaaat ccatcgacat ctgccctgcc gtcagtttcg
    11161 cttttaccac ctggtccgct gcaaaggcaa aaggctgtaa cggttttaag gtatggttaa
    11221 agcggtctgc gctttcaagg aacatctcgc cgccttccag caacgtcact atcctttcca
    11281 tgccgggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg gcgcgccagt
    11341 aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg gcagcgtttc
    11401 gccacagatt caccgacatt ttacgcatat caaagtattc catactcgct cccggcccgt
    11461 tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg ggtgatttac
    11521 gttagtggtg atcaggcttt ttttacaact gtttgagaag agaaaagaaa accgccgatc
    11581 ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg
    11641 aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc tgtcagacgc
    11701 ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt gtaaattttg
    11761 ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat ttttgcgatc
    11821 tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg cattgcgcgg
    11881 cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat ttcgcggatc
    11941 tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg atgcggaccg
    12001 aacttgcctt tgtggtgcat catcggcttc gcgtcagccg gtgctgcggt agtggtgtct
    12061 gcggcatggg ccaggttagc cgcgccaaga gccagggtag aggcaacaaa cagtgcagtt
    12121 aatttacgca tattctatat ccttcctttc agttatttat tacggctttc ttaagtagcg
    12181 tgccgtgttg acgagattaa ctttactgac tttagcgtca attaatcaga gcaacggtaa
    12241 aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag aaaatttgat
    12301 ggagagtgat gagagaataa tacaacacga tgattttgca gagattatga agaactatac
    12361 cggatgactg gtgataaata aagcaaataa ccaggattaa tctgtattaa tttataagaa
    12421 agcaacttaa tacccgcaga atgatttctg cgggtaagta ttagcttatt ttttcgagca
    12481 ttaatcccgc gcgtaatccc aacgctacca acggattagg gaataacaca tactctacat
    12541 catgggttac ggtaaaacgt tcctctccgt cctgcgccag caatgttcct ttctcaaacg
    12601 gcataaaatt cagcgtgtca cttgccatat gcatttcgaa ggacggcgag tggcgagtaa
    12661 tttgcgaaac cacccgataa cggagcggcg gtgttctcac gataccgaca ctctcaccag
    12721 atagcagcgc agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg ttttgcccaa
    12781 agggcaacgc tttgccaagt tccagcgtac aggccagcgc gccaaaatgt ctggcgctga
    12841 aatgggtaaa cgtaccacca ggttcctgat ggaacaccag cgcctccagc cccgccgcac
    12901 ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat acaccgaact
    12961 gcggatgcaa ggagccacga attgcggtat gtagatcaag gtgccagcgc acagattctt
    13021 tgcccaggtc ataaaaatct tccaggcact gttccagttc gcgcgcccga caggtttctc
    13081 cgctttcagc aaatagctgc caacgaccgc caaacattcg attcatatcg ctatggcaat
    13141 aacgtttccc ttgcttcagc gcaggaggat tcccgaggat caccagcaac cgccaacgta
    13201 acgggatctc gccgtgagat atcgcgccaa gcaacgcgtc cagcatctcc acaggtgccg
    13261 tctcattacc gtgtattccc gctgaaatca ccagtgcgcc ttgcggtggc gttaatggcg
    13321 tcagttccag cacaccatcg cccagccagc gccagcgaac gccgttgatt tctcgctcgg
    13381 tgataaccgg ttttttaccc gttaaggtca gagcaagaaa attatccatt gccccctccc
    13441 tcgcgctgga acggataaac cgaaccgaga ttcagtaatt ggctcaatac atccagtgct
    13501 tcccgccctc cgcgcagcaa ttgcgggtcg gccagatcgg cagcagtaag gcgatcgcgg
    13561 tagtaacgat ccacccagtc attgagcgca ttaaacagcg tatcgttcat catcaccgcc
    13621 ggattcaccg cccggcgttc ttcttctgtc aataccaccc gcaaccgcag gcacgccggg
    13681 ccgccgccat tcgccatgct ttcacgtaaa tcaaagactt ttagttcgct aatcgggttg
    13741 tcagcggcaa ggagttcatt gagataaccc catactccgg cgtgttcccg acactcctga
    13801 ggcagcacca acatcatgga accatcatcg cggctcaaca gttggctgtt aaacagatag
    13861 gtagacaccg catccgacac ggaaacctga gttgccggaa cttctatcgc cataaaaccg
    13921 ttcacccgcg cacgcaggtt tgccagtaac tgtgactggc gagcgaacgc ctgttggtgg
    13981 caaaacagca cctggcggtt actcacggca atcacgtcat tatgaaaaac gccctggtcg
    14041 ataacgtccg ggttttgctg ggcgaaaatc acctgttggg gattcacctg attcagcctt
    14101 gccaccgcct cgctggcttc gcgagtctgt cgcgccggat aacgggaagg ccgggtatca
    14161 ttgccttctt ctcgcccgta gacaaaaagt tgcatacccg gctcaccgta atgaccgccg
    14221 agacgattgt ggtttgccgc cccctcatca ccgagcaacg ctacctgcgg caacgccgaa
    14281 tgaacgctaa atttctcttc gtcgttaaaa atcgctttta aaagtgattc ggtgacgtgc
    14341 gcttccagcg agcggtgaaa tttattgttc aggttggcaa cggtgagatg cactttgcca
    14401 tccagcgtat cggcagatgg cgcgatcgtt gccgcattgg ctacccacat tggcgaagcg
    14461 gaactgacgc tggaaagcca gtgcggtgcc tggcgtgcaa ctttttccag tacctgctca
    14521 tcgctaccac tgaatcccaa ctgacgcagc accggaataa acggacgctc gtgcggcggg
    14581 atcacggcct gggggaatcc cgcatcggca agggctttca ttttcagtaa gccctgtttt
    14641 gccgcctgtc gcgggttaga cacctgaaaa cggtgacggg tagaggcttc attaccaaac
    14701 gacaggccag cgtaatgatg cgtcagccct accagcccgt cgaaattaac ttcccaggcg
    14761 ttcatcgcac cacctcatcg gaaaaatcca gcccggggtt aagcgttgcg ggcaaagtta
    14821 acgagtccga ctccaggctc gccataggcc atgcgcagta atctgcggca taccaggcgc
    14881 tggggcgatg gttaccggat gcaccaatgc cgccgaatgg cgcggtactg gcagcaccag
    14941 taagcggttt gttccagtta acaatccccg cccgcgcctc cagcaacagt tgatcgaatt
    15001 tttcccgctc gggggaaacc agaccgcaag agagtccgaa gcgagtgtta ttcgccatta
    15061 gaatcgcttc ctcgaaagaa tcataacgcc agacgcgcaa taacggtccg aacacctctt
    15121 catctggtac gccagcaacg cctgtcattt caatgatccc cggcgtcagc aacgatgtct
    15181 ctgattgtaa taagcgcggc gcaagcaggg ttcgtccgcc catcgcttcc agttgctgcc
    15241 aggcagtaac cacctgctgt gcggcctgtt cagaaatcag cccgccaata aacggctgcg
    15301 gttcgtcatc ccagttgccc ggcgttaatc gctggctgac ggcaaccaga cgagcaagaa
    15361 acgcatcgcc ctgcgctccg cttttgagca ataaacggcg ggcgcaggtg cagcgttgcc
    15421 cggctgtgac aaacgccgac tgaatggtca gatggacagc cgcgtcgata tccgccacct
    15481 catcgataat tagcggatta ttaccgccca tctcaagggc gagaattttc tccggctgac
    15541 cggagagctg gcgatgcagc tggtagccgg tattggcgct accggtaaac agcaaaccgt
    15601 cgagatcctc cagcgcactc agcgcctgac ccgtttcacg cccgccctgc accaggttca
    15661 gtacgcccgg cggcaagcca gcctgctgcc ataaacgcat taccgcttcg ccactccacg
    15721 gtgtcagttc gctgggttta aagataatgg tgttacctgc cagcaatgcc ggaacgatat
    15781 gtccgttcgg caaatgacca gggaaattat acggcccaaa caccgccagc acgccgtgcg
    15841 ggcgatgtcg caggctcgcc gcaccgtccg gcatttcact acgctgctcg ccggtacgag
    15901 cgtgatacgc cttaattgat atcgcgattt tattgatcat cgccgtcact tcggttgccg
    15961 cttcccagcg cggcttgccg gtttctcggg caatgattgc ggttaattcg cctttattcc
    16021 tttccagcaa tccggcaaag cgttcgacaa cgacctgacg ttcagccaat gagagccgcg
    16081 cccagcgcgg aaacgccgca cgggctgccc gacaagcctg ctcgacctga gcggcatcgg
    16141 catcattgcc ttgccataac acctcgcccg ataccggatt acgcttcaca cgcgatgcgc
    16201 cctggcccgt tatccagtca ccgttaatcc ataaagtcat gctgttttct cctctgcgca
    16261 caggcgcacc agacgaacgc gatcccctgc gtggcatttg agggcatcca gttgtgcggc
    16321 ggttaaaatc aaacgctcgg ttgccggatc ggtacgcgcc agcaccacgc ggaaatggtg
    16381 ataattttca ttggcgacca ggcaggccgg aaaatcgccc tgcgcaggct gcccttctgc
    16441 cacttccacc agccgacttt tacgaatggc gcgcacgcgg tcgatgtcac actcaagcgt
    16501 tggcccgccg tcgaagatgt cgatatagtt gcggtaacga aaaccttctt tctccagcac
    16561 cgcgcgggca ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg cttcctggga
    16621 taaaaagtgg gtatagatcg gatgtttcgg catcagttct gcaataaatg ccttttgccc
    16681 ggtgccgcag agaaaatcgg cgcggctaaa atccatcgaa aagaagcgtt taccgaggct
    16741 ttgccagaac ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcag caaccacttt
    16801 gtcattaaac ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg acagcaaata
    16861 gccgttgccc tctttgcgcc agtccgggtc gagaaacagc gtgcacagct cgctgctgcc
    16921 ggtgtgatcg ttactgagaa acagcgtcgg caatgcgtta tagacattca gctcttttga
    16981 ggcgtgaacc aacgtgccga cgcgatagtt gtaccaggga tcgttcagcc caaccgccac
    17041 ctcaatggca caaatccccg ccacggtgcc tgtctcgcta tcttccagca cgaacacata
    17101 gccctgctca cttttgggca gttcgccttg ccaggttttg attgcccttt cgatacgcgc
    17161 cgaaagcgtg gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt tgctggcaag
    17221 ctgcatcagc gccgagacat ctgatcgctc aacgggacgg atgaccatca tgatgaacct
    17281 cggctaacaa agtgttcgca agcagctgca aagcgatcca gtccggtcgt cacctcttct
    17341 tcgctgacat tgagcgcagg cgcaaaacgc accacgttgc cacccgcaat cagtaccatc
    17401 acgcttgctt tcgccgcttc ctgagagatc tgtttcgctt gcccggcgta atcggcattc
    17461 agtacacagc caatcagcaa acctaagccg cgaacttcac tgaacaaacc atagcggtga
    17521 ttaatagtat taagacgctc aacaaaccag tcgtgacgct gtttaacgcc attaagcatc
    17581 tctggtgtgt tgatgagctc cagcactttg cctgccaccg ccgaggccag cggattaccg
    17641 ccataggtgg tgccatgagt gccaacggtc atcacgcggg cgcactcttc ggttgccaac
    17701 aacgcaccga ccgggaaacc gccgcccagc gctttggcgg tagttaacag atcaggcgtc
    17761 acgccgtagt gcatataggc atacagttcc ccggtgcgcc cgacgccggt ttgtacttca
    17821 tcaaaaatca acagcgcatt gtggcggtta cacaattcac gcagaccttg taaaaacgcg
    17881 ttgctggctg gcaccacacc gccttccccc tggatgggtt cgacaatcac cgcacaggta
    17941 gagtcgtcaa tcagcgcgct ggcagagtta atatcgttat atgcagcatg acgaatatcc
    18001 gccggcagtg gcgcaaaatc ctgtgaatag gctggctgcc cacccgcact gacagtaaac
    18061 agcgtgcgac catgaaacgc atttttgaac gccacgatgc cgctcttatg gctgccgtag
    18121 cggtcgtgag cgaatttacg cgccagtttt agcgccgctt cgttggcttc cgcaccggag
    18181 ttacaaaaga agacgcgatc ggcaaacgtg gcgtcgatca attttttcgc cagtcgcagt
    18241 accggctcgt tggtgtaacc gttgccggta tgccagaact tactcgcctg ttcgttcagc
    18301 gcttcacgca gttccggatg cgcatggccc agcgcgttca ccgcaatgcc acccgcgaag
    18361 tcgatatact ctttcccctg ctgatcccac aagcgcgaac cttcgccacg taccggtata
    18421 aagggtgccg gagcgtaaac aggtatcatc cattcatcaa agttttcacg cgtaattggc
    18481 tgagacatag cgacctctat agtaaataat tcgttattta tatgttaaca ataagtaatg
    18541 tttgcgctgt aaatgtagat tgcagggttc gtgccagcca gtgataaaag tgcataaacg
    18601 gcggaggcta actggaaatc aaggagttat aaccaaacca tatgcattta aagtgcatat
    18661 aaagtgaata cgtttgcgat gtgggtgaat aaaaagaata aaaaacgcaa tgttatgcag
    18721 aagtaaaata taattctgga attgtgatca ttgacgaaat ttactggaaa ttactgcgcc
    18781 attctgacgc agcgcgcacc aaaagcgggc attttttgcg ccatcgttga catcattaac
    18841 aaccatcgat caaatcactt aacaacaggc ggtaagcaac gcgaaattct gctaccatcc
    18901 acgcactctt tatctgaata aatggcagcg actatgaaat ttgtctcttt taatatcaac
    18961 ggcctgcgcg ccagacctca ccagcttgaa gccatcgtcg aaaagcacca accggatgtg
    19021 attggcctgc aggagacaaa agttcatgac gatatgtttc cgctcgaaga ggtggcgaag
    19081 ctcggctaca acgtgtttta tcacgggcag aaaggccatt atggcgtggc gctgctgacc
    19141 aaagagacgc cgattgccgt gcgtcgcggc tttcccggtg acgacgaaga ggcgcagcgg
    19201 cggattatta tggcggaaat cccctcactg ctgggtaatg tcaccgtgat caacggttac
    19261 ttcccgcagg gtgaaagccg cgaccatccg ataaaattcc cggcaaaagc gcagttttat
    19321 cagaatctgc aaaactacct ggaaaccgaa ctcaaacgtg ataatccggt actgattatg
    19381 ggcgatatga atatcagccc tacagatctg gatatcggca ttggcgaaga aaaccgtaag
    19441 cgctggctgc gtaccggtaa atgctctttc ctgccggaag agcgcgaatg gatggacagg
    19501 ctgatgagct gggggttggt cgatactttc cgccatgcta atccgcaaac agcagatcgt
    19561 ttctcatggt ttgattaccg ctcaaaaggt tttgacgata accgtggtct gcgcatcgac
    19621 ctgctgctcg ccagccaacc gctggcagaa tgttgcgtag aaaccggcat cgactatgaa
    19681 attcgcagca tggaaaaacc gtccgatcac gcccccgtct gggcgacctt ccgccgctaa
    19741 tttagcagct ctcctggctc aaactgggtc aggagaatta accttgagaa aaatcaacaa
    19801 actgtcagta atgatttgtt gcctgccgtc ctttgttata ccgtcactgc gtttttagtt
    19861 gtctgaccac ttctctatta tcaagtttga tataggaaac tccacgatga acgctgagcg
    19921 taaatttctt ttggcctgtc ttatttttgc gctggtcatt tacgctatcc acactttcgg
    19981 tttattcgat ctgctcaccg atttacccca cttacagaca ctcatccgcc agagcggatt
    20041 gttcggttat agcctctata ttctgttatt cattattgcc actctctttc tgttaccagg
    20101 aagcatactg gtgatcgctg gaggaatagt ttttggcccg ctcttaggga cactactctc
    20161 attaattgcc gccacgctcg cctcctcgtg ctcattcctg ctggcgcgct ggctgggacg
    20221 tgatttactg ctgaaatacg ttggtcatag ccataccttt caggccattg aaaaaggcat
    20281 tgcgcgtaac ggtattgatt ttcttattct gacccgctta atcccgttgt ttccttacaa
    20341 tattcaaaat tatgcttacg gattaaccac aatcgccttc tggccttata cccttatttc
    20401 ggcactcacg accctgcccg gtattgttat ttataccgtg atggcaagcg atctcgccaa
    20461 tgaaggcatt acgctgcgct ttattttaca actctgtctg gcgggcctgg cgctgtttat
    20521 tctcgtccag ctcgcaaaac tctacgcccg acacaaacat gtggatctgt ctgcttcgcg
    20581 ccgcagccca cttactcacc caaaaaatga aggatagaac gatgtcgcaa cattattcag
    20641 tgtcatggaa aaaaggactg gctgcactct gcttactggc tgttgccggg cttagcggct
    20701 gcgatcaaaa agagaatgcg gcggcaaaag tggaatacga cggactttcg aacagccaac
    20761 cgctgcgcgt cgatgccaat aaccatacgg taaccatgct ggtgcaaatt aatggtcgtt
    20821 tcctcaccga cgacactcgt cacggtattg tgtttaaaga tggctccaac ggacataaat
    20881 cgctgtttat ggcttatgcg accccgaaag cattttatga agccctgaaa gaggcaggtg
    20941 gtacgccggg cgaaaacatg acgatggata ataaagaaac gactcatgtc acaggcagca
    21001 aactggatat ttcggtcaac tggcaagggg cggcaaaagc gtattccttc gatgaagtga
    21061 ttgttgacag taatggcaag aaactggaca tgcgctttgg cggtaattta acggcagcag
    21121 aagagaagaa aacgggttga ctggtgtgtc tggatagctg cccggtcggc atcgtcagca
    21181 atgcaacata cacttatggt gcggtggaaa aacgcggcga agtgaaattc aaaggcaatg
    21241 cctcagttct cccggcggat aacacgctgg caacggttac ctttaaaatc accgaataaa
    21301 gccaggataa aggatgatga agatacaatc gcgtaaaatc tggtactacc gtataaccct
    21361 catcatcctg ttgttcgcta tgctgctcgc atgggcgctg cttcccggcg tccatgagtt
    21421 tatcaatcgc agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag aacgctttat
    21481 tcagtcttac ggcgcactag cggcagttgt ctcgttcttg ttgatgattt tacaggccat
    21541 tgctgcaccg ctacctgcgt ttttgatcac ctttgccaat gcgtcgctgt ttggcgcgtt
    21601 ctggggcggc ttgctgtcgt ggaccagttc gatggccggc gcggcgctgt gcttttttat
    21661 cgccagagtg atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg tgcttgacag
    21721 tatggacggc tttttcactc gctacggcaa acacaccatc ctggtctgtc ggttattgcc
    21781 ttttgtccct ttcgatccaa tcagctatgc tgccggtctg acttcaatac gttttcgctc
    21841 gttttttatc gccaccgggc ttggtcaatt accggcgact attgtttatt cctgggcggg
    21901 cagcatgtta acaggcggta ctttctggtt tgtcaccgga ctgtttattc tgtttgccct
    21961 gaccgtggtg atttttatgg cgaagaaaat atggcttgag cgccagaaga ggaatgcctg
    22021 atgggtttac cgccgcttag caaaattcct ttcattttac gtccgcaggc gtggctgcat
    22081 cgtcgccatt acggcgaggt gctaagcccg attcgctggt gggggcggat cccgtttatc
    22141 ttttatttgg tgtcaatgtt tgttgggtgg ctggagcgca aacgctcacc gctcgatccg
    22201 gtagtgcgat cgcttgtcag cgcgcgcatt gcgcaaatgt gcctgtgtga gttttgcgtg
    22261 gatatcacca gtatgaaagt cgccgagcgt accggcagca ccgataaact gctggcagtg
    22321 gctgactggc ggcaaagccc gctcttcagc gatgaagaac ggctggcgct ggagtacgcc
    22381 gaagccgcaa gcgtaacgcc gccaacggtc gatgatgccc tgcgcacccg actggctgcg
    22441 cattttgacg ctcaggcgct caccgaactg acggcattga tcggcctgca aaacctgtcg
    22501 gctcgtttta attctgccat ggacattccc gctcaggggc tgtgccgtat tccggaaaaa
    22561 cgttcttaag gagagaagat gcgccattgt gggtggttgc tgggattgtt atcgctgttt
    22621 tctctggcaa cacatgccag tgactggcag gaaattaaaa atgaggccaa agggcaaacc
    22681 gtctggttta acgcctgggg cggcgatacc gcaattaacc gctatctcga ctgggttagc
    22741 ggcgagatga aaacccatta cgctataaac ctgaagattg ttcgtctggc ggatgccgca
    22801 gacgcggtga agcgcattca gaccgaagct gctgccggac gtaaaacggg cggctcggtg
    22861 gatctgctct gggtgaacgg cgaaaacttc cgcaccttaa aagaggccaa tttattacaa
    22921 acgggctggg cggagactct gcccaactgg cgctatgtcg acacacagct gccggtgcgg
    22981 gaagattttt cagtgcctac agaaggggct gaatcgccct ggggcggcgc acaactgacg
    23041 tttatcgccc gccgcgatgt tacgccacag ccaccacaaa cgccgcaagc cttactggag
    23101 tttgctaaag ccaatcccgg cacggttacc tatccgcgcc caccggactt taccggcacg
    23161 gcgtttcttg aacagttgct gattatgctg acgcccgatc ccgccgcgtt aaaagttgcc
    23221 ccggataaag ccactttcgc ccgcgtcact gcgcccttgt ggcaatatct tgacgcgcta
    23281 catccctatt tatggcgcaa aggaaaggat ttcccgcctt cgcccgcgcg gatggatgct
    23341 ctgctgaaag ccggcacatt gcgcctgtcg ctgaccttta accctgctca tgcccagcaa
    23401 aaaatcgcca gcggcgattt gcctgcaagc agttacagtt ttggttttaa ccaggggatg
    23461 attggcaacg tgcatttcgt caccattcct gctaacgcaa atgccagtgc tgcggcgaag
    23521 gtggttgcca acttcctgct ctcacccaat gcgcaactgc gtaaagcgga tcccgctgtc
    23581 tggggcgatc cttctgttct cgatccgcaa aaactgcctg atgggcagcg cgaaacattg
    23641 caatcaagaa tgccgcaaga tctgccgccg gtactggctg aaccgcacgc aggttgggta
    23701 aatgcgctgg aacaagaatg gctacaccgt tacggtacgc attaatcttt ttgctgtggg
    23761 cgatggtggc ggtgatttat gcaccactga tcccggcagc tctcacgctg atatcgcctg
    23821 ctttgtcgtt gacacactgg caggcgttat ttgccgatcc acagttaccg caagcattac
    23881 tggcaacgct ggtgtcgaca accatcgcgg cggtcggggc gttgttgatt gccctgctgg
    23941 tgattgtggc gctgtggcct tggccgaaat ggcagcgtat gtgtgcccgt ctgccgtggc
    24001 tgctcgccat tccccatgtg gcttttgcca ccagcgccct tctgctcttt gctgacggag
    24061 ggctgcttta tgactatttc ccgtatttca ctccgctaat ggacaaattg ggtattgggc
    24121 tgggcctcac ccttgcagtg aaagaaagcg catttctgct gtggatctta gcggcagtat
    24181 tgagcgaaaa acggctgttg cagcaggtca ttgtgctgga ttcgctgggc tacagccgct
    24241 ggcaatgcct gaactggctg ctgttgccct ccgtcgcacc tgcactggca atggcgatgt
    24301 tggcgattgt tgcctggtcg ctttcggtcg tggatgtggc gattattctt gggccaggta
    24361 atccaccgac gctggcagta attagctggc agtggttaac tcagggcgac gcggatcaac
    24421 aaacgaaagg cgcactcgcc agtctgctgc tgatgctgtt actcgccgcc tacgttttgc
    24481 tgagctatct gctatggcgc agctggcggc gcactattcc ccgcgtagat ggcattcgca
    24541 agcctgccac acctttatta ccaggcacta cgctggcgag ttttttaccc ttaaccggtg
    24601 tgctgtgtgt ggttctgctg gcgatcctcg cggatcagtc gacgatcaat agtgaagcac
    24661 tcatcaacag cctgacaatg gggctggtgg cgacattcat cgctttgctc ctgttactgc
    24721 tgtggctgga atgggggcca cagcgtcgcc agttgtggct ctggttgccc attctattgc
    24781 ccgctctgcc actggtggcg ggccagtaca cgctggcgct atggctgaac ctggatggaa
    24841 gctggacggc ggtggtctgg gggcatctgc tgtgggtaat gccgtggatg ctgtttatcc
    24901 tgcaacccgc ctggcagcgc attgattcac ggttaatttt gattgcgcaa acactgagct
    24961 ggtcgcgggc caaaatcttc ttttacgtga aatgtccgct tatgctacgc cctacgctga
    25021 ttgccttcgc ggtgggattt tcagtcagta ttgcgcagta tatgccgacg ctgtggctgg
    25081 gcgcggggcg ttttccgacg ctcaccactg aggcggtggc attaagcagc ggcggcagca
    25141 atggtatttt cgccgcccag gctttatggc aactgctatt accgcttatt atttttgccc
    25201 tgaccgcgtt agtcgcaaaa tgggtaggtt atgtcagaca aggactccgc taatgctctg
    25261 cgtgaaaaat gtttcgctac gtttaccaga aagccgcttg ctgacaaacg ttaactttac
    25321 ggtggataaa ggtgacattg tcacgttaat ggggccgtct ggctgtggaa aatccactct
    25381 gttttcatgg atgattggtg cactggctgg acagttttct tgtacaggtg agctatggct
    25441 caatgagcaa cggattgaca tgctacccac cgcacagcgt cagattggca ttcttttcca
    25501 ggatgcactg ttatttgacc agttcagcgt cgggcaaaat ttactgctgg cgctaccgtc
    25561 gacgctcaag gggactgcca ggcgcaatgc cgtgaaagac gcgcttgatc gagcgggact
    25621 ggctgaaact tatcaccagg accctgcgac attatcaggc ggtcagcgag cgcgggtggc
    25681 cctgctgcgc gcccttctcg ctcagccaaa agcgttactc ctggatgagc cattcagccg
    25741 tcttgatgtg gctctgcgcg ataattttcg ccagtgggtg ttcagcgaag ttcgcgaact
    25801 ggcgatcccc gtcgttcagg taacgcacga tctccaggat gttcctgctg atagttctgt
    25861 tctggatatg gcgcagtggt cagaaaatta caacaaactg cgataacgca aagtttttct
    25921 caatgcgtca gttcagaatg gcgcactcaa aactacaatg tcgggatttt cgatgaaacg
    25981 tgtttctcaa atgaccgcgc tggcaatggc tttagggctg gcttgcgctt cttcgtgggc
    26041 cgctgaactg gcgaagcctc ttacacctga ccagcttcaa caacaaaatg gcaaagcgat
    26101 agatactcgc cccagcgcgt tttataacgg ctggccacaa accttaaatg gcccttctgg
    26161 tcatgaacct gccgccttaa acctctctgc cagctggctc gacaaaatga gcaccgaaca
    26221 gctcaacgcg tggatcaagc aacataacct gaaagccgat gccccggtgg cgctgtacgg
    26281 taatgacaaa gatgtcgacg ccgtcaaaac gcgactgcaa aaagcaggtt ttacgcatat
    26341 ctccattctg agtgacgcgc taagcgaacc ttcccgtcta caaaaactgc cgcactttga
    26401 gcagctggtt tatccgcaat ggctgcacga cctgcaacaa ggtaaagagg ttacggcgaa
    26461 acctgccggt gactggaaag tcattgaagc ggcctggggc gctcctaagc tttaccttat
    26521 cagccatatt cccggtgctg actacatcga taccaacgaa gtggaaagcg aaccgctgtg
    26581 gaacaaagtt tctgatgaac aactaaaagc gatgctggca aaacacggca ttcgccatga
    26641 caccacggtc attctgtacg ggcgtgacgt atacgctgga gcgcgtgtgg cgcaaattat
    26701 gctctacgca ggcgtgaaag atgtgcgtct gctggatggc ggctggcaaa cctggtccga
    26761 cgcgggactg cctgttgagc gaggaacgcc accgaaagtg aaagcggaac cggatttcgg
    26821 cgtgaagatc ccggcacaac cgcagttgat gctcgatatg gaacaggccc gtggactgct
    26881 gcatcgccag gatgcatcgc tggtgagcat tcgttcgtgg ccagaattta tcggtacgac
    26941 cagcggttac agctatatta aaccaaaagg tgaaatagcc ggagcacgtt ggggacacgc
    27001 tggtagcgac tcgacgcata tggaagattt tcataacccg gatggcacca tgcgcagcgc
    27061 cgatgatatt accgctatgt ggaaagcgtg gaatatcaaa ccagagcagc aagtttcatt
    27121 ctactgcggc accggctggc gcgcgtccga aacctttatg tacgcacgcg ccatgggttg
    27181 gaagaatgtt tccgtgtatg acggcggctg gtacgaatgg agcagcgatc caaaaaatcc
    27241 ggtagcaacc ggtgaacgcg gcccggacag tagcaaataa cattgcaatt actgacgctg
    27301 gtgcgacttc agcgtcagat acccactcca cacccgcgta aaggtcgtca tccagcacaa
    27361 cgcaccgaag atccacgcaa accacgcaaa ccatgcaggg aataaacatc ccagcacaaa
    27421 cagtaagatc gtttctgtgc cttcggttaa cccgcccaga taataaaacg atttatgcgc
    27481 ataaccggga ttatcaatct gatgtttcgc cgccagcgca gcaaaagcga gaaaactgct
    27541 gcctgtgccg ataaacgcaa acagcagcca gccgcctgcc agggcgtttt gctgcggtgc
    27601 ggcgaggata aacccaaaag gtaccagcgc gtaaaaaaga aaatcgagag aaatatcaag
    27661 aaagccgccc gcgtcggtaa gccctctgcg ccgcgccagc gcaccgtcca gaccatcaag
    27721 cagcctgttc aacaaaatga cgacaagcgc cgccagatac cagcccagcg ccagaaaagg
    27781 cagtgccagc acgccgatgg caaatcccac taatgttaaa ccatctggcg taatgcccgg
    27841 tttatcaaga acccgcacgc actgatgcaa caacggttta atccggggat gaagatggcg
    27901 gtctagcaca ggcactcctt aaatataaag cctttctgat tgagcaacag tgcggatatt
    27961 atggcatttt tcgcttatct gcccgtgtgt aatttatgaa aatgattgaa gttgttgccg
    28021 ccatcattga acgtgatggc aaaattttac tcgcgcaacg ccccgcccag agcgatcagg
    28081 cgggattatg ggagtttgcc ggtggtaaag tcgagccgga tgaaagccag cggcaggcgc
    28141 tggtgcgtga gttacgcgaa gaactgggca tcgaagcaac tgtgggtgag tatgttacca
    28201 gccatcagcg agaagtttcg ggccggatta tccatcttca tgcctggcac gtacccgact
    28261 tccacgggac gttacaggca catgaacatc aggcgctggt ctggtgctca cctgaagagg
    28321 cgctgcaata tccgctggcc cctgctgaca ttccattatt agaggcgttt atggctttac
    28381 gcgccgccag accagcggat tagtgctaag ggttttgtca tcccgctagc attgcagcag
    28441 tattccctcg gctttaatta ccgcgccttc agaataattt ttatcctgat aaacgcagca
    28501 ctgagtacag ggctgcgctg actgcccgcc tgaactgaac acttctggcg gtacgtttac
    28561 ctccacgtcc ggacgataat gcgggttagc cagtgcaatt aatggaaatg ctaatactac
    28621 ggcgaacaat gctcgactca cagggaactc cttaacgtta ttgtctctgc tactgataac
    28681 ggtagccggg tggcaaaact ttagcgtctg agttatcgca tttggttatg agattactct
    28741 cgttattaat ttgctttcct gggtcatttt tttcttgctt accgtcacat tcttgatggt
    28801 atagtcgaaa actgcaaaag cacatcacat aaacaacata agcacaatcg tattaatata
    28861 taagggtttt atatctatgg atcagacata ttctctggag tcattcctca accatgtcca
    28921 aaagcgcgac ccgaatcaaa ccgagttcgc gcaagccgtt cgtgaagtaa tgaccacact
    28981 ctggcctttt cttgaacaaa atccaaaata tcgccagatg tcattactgg agcgtctggt
    29041 tgaaccggag cgcgtgatcc agtttcgcgt ggtatgggtt gatgatcgca atcaggtaca
    29101 ggtcaaccgt gcatggcgtg tgcagttcag ctcagccatc ggcccgtaca aaggcggtat
    29161 gcgcttccat ccgtcagtta acctctccat tctcaaattc ctcggcttcg aacaaacctt
    29221 caaaaatgcc ctgactaccc tgccgatggg cggtggtaaa ggcggtagcg attttgaccc
    29281 gaaaggtaaa agcgaaggcg aagtgatgcg tttctgccag gcgctgatga ccgagcttta
    29341 tcgtcacctg ggcgcggata ccgacgttcc ggcaggtgat atcggcgttg gtggtcgtga
    29401 agtcggcttt atggcgggga tgatgaaaaa gctctccaac aataccgcct gcgtcttcac
    29461 cggtaagggc ctttcatttg gcggtagtct tattcgcccg gaagctaccg gctacggtct
    29521 ggtttatttc acagaagcaa tgttaaaacg ccacggtatg ggttttgaag ggatgcgcgt
    29581 ttccgtttct ggctccggca acgtcgccca gtacgctatc gaaaaagcga tggaatttgg
    29641 tgctcgtgtg atcactgcgt cagactccag cggcactgta gttgatgaaa gcggattcac
    29701 gaaagagaaa ctggcacgtc ttatcgaaat caaagccagc cgcgatggtc gagtggcaga
    29761 ttacgccaaa gaatttggtc tggtctatct cgaaggccaa cagccgtggt ctgtaccggt
    29821 tgatatcgcc ctgccttgcg ccacccagaa cgaactggat gttgacgccg cgcatcagct
    29881 tatcgccaat ggcgttaaag ccgtcgccga aggggcaaat atgccgacca ccatcgaagc
    29941 gactgaactg ttccagcagg caggcgtact gtttgcaccg ggtaaagcgg ctaatgctgg
    30001 cggtgtagca acgtcaggcc ttgaaatggc gcaaaacgct gcgcgcctgg gctggaaagc
    30061 cgagaaagtt gacgcacgtt tgcatcacat catgctggat atccaccatg cctgtgttga
    30121 gcatggtggt gaaggtgagc aaaccaacta cgtgcagggc gcgaacattg ccggttttgt
    30181 gaaggttgcc gatgcgatgc tggcgcaggg tgtgatttaa gttgtaaatg cctgatggcg
    30241 ctacgcttat caggcctaca aatgggcaca attcattgca gttacgctct aatgtaggcc
    30301 gggcaagcgc agcgcccccg gcaaaatttc aggcgtttat gagtatttaa cggatgatgc
    30361 tccccaggga acatttctta tgggccaacg gcatttctta ctgtagtgct cccaaaactg
    30421 cttgtcgtaa cgataacacg cttcaagttc agcatccgtt aactttctgc gatagcagca
    30481 gatatgccag taaagaaatc ccattagact atttttttga taatcttctt cgctttcgaa
    30541 caactcgtgc gcctttcgag aagcaagcat tatataatgc caggccagtt cttcttcaat
    30601 tgtcccgttt tgaaaagctg tgcttgatat cgagatcatc catgataatt ccgccgccca
    30661 tattagcttc gccgaggatt taccggagcg attattagcg caatcagaga tatagtctga
    30721 gggaaaaaca gcaaatttat tcaacaaggc gataacctgc tctggggctt cctccatgtt
    30781 tgctttaaag gtattggctc catggtcgcc agaaagaaaa tgctccatta aggcacaata
    30841 actttcgcta tcttcgatac cccattgatc ctctaaagac tcgcgtcttt tacttatgat
    30901 atcgatcgag tcaaaaggaa gcacatgata ttggaaggta tctttgccag gttcaggctt
    30961 tcgcggccag aactccagcg tttcagacca ttgcttatga tagaatcgat aaggtgcgat
    31021 caattgtagc gcctgtaact tctcgatact gagcggctca atacctttag cctgataata
    31081 atgcagttgt tctttttttg ctttaaaacc ggcccgaaca ataagcccca tcataattaa
    31141 tagataaaga aaagagcatc ccgcggtaat caggcctctt tcattcaaac cgttggatgt
    31201 tatcgctgcg aacacaaaca ttacagcgac aacacatgtt aaataaaacc cccacttaca
    31261 aagcagcatg
//
LOCUS       DAPHDX010000046        30631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPHDX010000046 DAPHDX010000000
VERSION     DAPHDX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.046"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(122..547)
                     /gene="exbD"
                     /locus_tag="RH058_002029"
     CDS             complement(122..547)
                     /gene="exbD"
                     /locus_tag="RH058_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV3137619.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(554..1288)
                     /gene="exbB"
                     /locus_tag="RH058_002030"
     CDS             complement(554..1288)
                     /gene="exbB"
                     /locus_tag="RH058_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV3137620.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            1540..2727
                     /gene="metC"
                     /locus_tag="RH058_002031"
     CDS             1540..2727
                     /gene="metC"
                     /locus_tag="RH058_002031"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV3137621.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGV
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2867..3526
                     /locus_tag="RH058_002032"
     CDS             2867..3526
                     /locus_tag="RH058_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3137622.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVISFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(3566..4465)
                     /gene="yqhC"
                     /locus_tag="RH058_002033"
     CDS             complement(3566..4465)
                     /gene="yqhC"
                     /locus_tag="RH058_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV3137623.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            4659..5822
                     /gene="yqhD"
                     /locus_tag="RH058_002034"
     CDS             4659..5822
                     /gene="yqhD"
                     /locus_tag="RH058_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV3137624.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            5927..6754
                     /gene="dkgA"
                     /locus_tag="RH058_002035"
     CDS             5927..6754
                     /gene="dkgA"
                     /locus_tag="RH058_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV3137625.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            6954..7880
                     /locus_tag="RH058_002036"
     CDS             6954..7880
                     /locus_tag="RH058_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV3137626.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            7931..8188
                     /gene="yqhH"
                     /locus_tag="RH058_002037"
     CDS             7931..8188
                     /gene="yqhH"
                     /locus_tag="RH058_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV3137627.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(8231..10450)
                     /locus_tag="RH058_002038"
     CDS             complement(8231..10450)
                     /locus_tag="RH058_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV3137628.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(10561..11973)
                     /gene="ftsP"
                     /locus_tag="RH058_002039"
     CDS             complement(10561..11973)
                     /gene="ftsP"
                     /locus_tag="RH058_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV3137629.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(12048..12785)
                     /gene="plsC"
                     /locus_tag="RH058_002040"
                     /gene_synonym="parF"
     CDS             complement(12048..12785)
                     /gene="plsC"
                     /locus_tag="RH058_002040"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV3137630.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(13019..15277)
                     /gene="parC"
                     /locus_tag="RH058_002041"
     CDS             complement(13019..15277)
                     /gene="parC"
                     /locus_tag="RH058_002041"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV3137631.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(15823..16305)
                     /locus_tag="RH058_002042"
     CDS             complement(15823..16305)
                     /locus_tag="RH058_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3137632.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(16358..16750)
                     /gene="ygiW"
                     /locus_tag="RH058_002043"
     CDS             complement(16358..16750)
                     /gene="ygiW"
                     /locus_tag="RH058_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV3137633.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            16902..17561
                     /gene="qseB"
                     /locus_tag="RH058_002044"
     CDS             16902..17561
                     /gene="qseB"
                     /locus_tag="RH058_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV3137634.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            17558..18907
                     /gene="qseC"
                     /locus_tag="RH058_002045"
     CDS             17558..18907
                     /gene="qseC"
                     /locus_tag="RH058_002045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV3137635.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(18953..19285)
                     /locus_tag="RH058_002046"
     CDS             complement(18953..19285)
                     /locus_tag="RH058_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDV3137636.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            19604..20185
                     /gene="mdaB"
                     /locus_tag="RH058_002047"
     CDS             19604..20185
                     /gene="mdaB"
                     /locus_tag="RH058_002047"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV3137637.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            20216..20530
                     /locus_tag="RH058_002048"
     CDS             20216..20530
                     /locus_tag="RH058_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV3137638.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(20578..22470)
                     /gene="parE"
                     /locus_tag="RH058_002049"
     CDS             complement(20578..22470)
                     /gene="parE"
                     /locus_tag="RH058_002049"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV3137639.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(22499..23080)
                     /gene="yqiA"
                     /locus_tag="RH058_002050"
     CDS             complement(22499..23080)
                     /gene="yqiA"
                     /locus_tag="RH058_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV3137640.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(23080..23907)
                     /gene="cpdA"
                     /locus_tag="RH058_002051"
     CDS             complement(23080..23907)
                     /gene="cpdA"
                     /locus_tag="RH058_002051"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV3137641.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(23932..24354)
                     /locus_tag="RH058_002052"
     CDS             complement(23932..24354)
                     /locus_tag="RH058_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV3137642.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(24355..24984)
                     /gene="nudF"
                     /locus_tag="RH058_002053"
     CDS             complement(24355..24984)
                     /gene="nudF"
                     /locus_tag="RH058_002053"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV3137643.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            25189..26670
                     /gene="tolC"
                     /locus_tag="RH058_002054"
     CDS             25189..26670
                     /gene="tolC"
                     /locus_tag="RH058_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV3137644.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            26818..27489
                     /locus_tag="RH058_002055"
     CDS             26818..27489
                     /locus_tag="RH058_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV3137645.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            27495..28655
                     /locus_tag="RH058_002056"
     CDS             27495..28655
                     /locus_tag="RH058_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV3137646.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(28693..29481)
                     /gene="ygiD"
                     /locus_tag="RH058_002057"
     CDS             complement(28693..29481)
                     /gene="ygiD"
                     /locus_tag="RH058_002057"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV3137647.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            29624..30397
                     /gene="zupT"
                     /locus_tag="RH058_002058"
     CDS             29624..30397
                     /gene="zupT"
                     /locus_tag="RH058_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV3137648.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
BASE COUNT         7551 a         8157 c         7687 g         7236 t
ORIGIN      
        1 ttgagcacct aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa cgcacgaaga
       61 ttcaacatgt tgcaatagat attgtaggcc tgatgagcgt agcgcatcag gcaattctac
      121 tttacttcgc tttggcggtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat
      181 gcagcgtatc cattaccttc atcaacgtct cgtaatcgac ggttttatcc gctcggaaga
      241 agatggtggt gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat
      301 cggtgaccgg atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg
      361 gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta
      421 ccgtcgctaa cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa
      481 acggcgtcac gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca
      541 ttgccatcgg atattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg
      601 cgctggcttc cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac
      661 ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag
      721 gaatcgctgc cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa
      781 cgactgccag gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc
      841 agaccgtacc aaacagacca acgaacggag aaatcgcgcc gatggttgcc agatagccgt
      901 taccgcggcc catttgacga cccactgcgg cgacccgacg ctccagacgg aagctggtac
      961 gttctttaat accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt
     1021 cattgagcaa atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct
     1081 ggtttaagga acgcgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat
     1141 taaagaactc tacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa
     1201 gcccaatcat cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa
     1261 ggtccgtctg cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct
     1321 gcgcataata atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac
     1381 tgccgtacct ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca
     1441 tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct
     1501 agtttagaca tccagacgta taaaaacagg aatcccgaga tggcggacaa aaagcttgat
     1561 actcaactgg tgaatgcagg acgcagcaaa aaatacactc tcggcgcggt aaatagcgtg
     1621 attcagcgcg cttcttcgct ggtctttgac agtgtggaag ccaaaaagca cgcgacgcgc
     1681 aatcgcgcca atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttctcctta
     1741 caacaagcga tgtgtgaact ggaaggtggc gcaggctgcg cgctatttcc ctgcggggcg
     1801 gcagcggttg ctaattccat tcttgctttt gtcgaacagg gcgttcatgt gttgatgacc
     1861 aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta
     1921 acgacatcgt ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac
     1981 actaaaatcg tgtttctgga atcgccaggc tccatcacca tggaagtcca cgacgttccg
     2041 gcgattgttg ccgccgtacg cagtgtggtg ccggatgcca tcattatgat cgacaacacc
     2101 tgggcagccg gtgtgctttt taaggcgctg gattttggca tcgatgtttc tattcaagcc
     2161 gccaccaaat atctggttgg gcattcagat gcgatgattg gcactgccgt gtgcaatgcc
     2221 cgttgctggg agcagctacg ggaaaacgcc tatctgatgg gccagatggt cgatgccgat
     2281 accgcctata taaccagccg tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat
     2341 gaaagcagtc tgaaagtggc tgaatggctg gcagaacatc cgcaagttgc gcgagttaac
     2401 caccctgctc tgcctggcag taaaggccac gaattctgga aacgagactt tacaggcagc
     2461 agcgggctat tttcctttgt gcttaagaaa aaactcaata atgaagagct ggcgaactat
     2521 ctggataact tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc
     2581 ctggcgaatc aaccagaaca tatcgccgcc attcgccccc aaggcgagat cgattttagc
     2641 gggaccttga ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg
     2701 gacgccggtt ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt
     2761 aattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gaatcaggag
     2821 tacaataggc agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca
     2881 agatatcatc gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt
     2941 tagcgttgtt tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc
     3001 ctcatttttg ccaggcgaca gcttgttgat actggcaggc gcattgattg cccaaggggt
     3061 tatggatttt ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct
     3121 aagttatatt caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca
     3181 gcttcctgct aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc
     3241 gctgctggct ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat
     3301 ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg ttaagtggat tgctgtgggt
     3361 cagcgtggta atcagttttg gctatgcctt aagtatgatt ccgttcgtta aacgccatga
     3421 agatcaggta atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt
     3481 aggcacgctg tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt
     3541 cccggctgct gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat
     3601 cttcccccgg cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt
     3661 catagccgac gcgcatcgct gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc
     3721 gcgccttatg cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact
     3781 taaaattatg gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc
     3841 tcaggttttc ggtgtattta ttctcaatcc gtttcagcac gcggctaatc agactgaagt
     3901 gagtctggcg actgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt
     3961 acagaatttc gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca
     4021 cgtcgagtaa ccgctccgcc gcgcataaaa tctcttctga taacgtggcg gagttaatcc
     4081 cgctggctgc catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct
     4141 gtaactgcaa aatatcgaca ttgagacgca accctgccag cggcacctct gacgtggcat
     4201 aggtttcgca ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac
     4261 gaaacacgcg ttcattgata taaccgattt tatgcccgga aaagagaatt atgatgccag
     4321 gctcgtacat caccggtgta cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg
     4381 gcaacagttc tgacaaacta ttttctttat ttttcagttt attaacttta tccgccagca
     4441 agcggcaaat ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg
     4501 attgtgcgca attttgtagc atttctccag cactctggag aaataggcaa gacattggca
     4561 gaaatgagca ttgagagcca gggcgctggc gatcacaatg aaaaacatca ggcagatcgt
     4621 tctctgccct catattggcc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca
     4681 ccccaacccg cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc
     4741 acgatgctcg cgtattgatt acctacggtg gcggcagcgt gaaaaaaacc ggcgttctcg
     4801 atcaagttct ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa
     4861 acccggctta tgaaacgctg atgaacgccg tgaaactggt tcgcgaacag aaagtgactt
     4921 tcctgctggc ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg
     4981 ctaactatcc ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta
     5041 aaagcgccat cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg
     5101 caggcgcggt gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg
     5161 ttcagccggt atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg
     5221 tggctaacgg cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg
     5281 ttgatgccaa aattcaggac cgtttcgcag aaggcatttt gctgacgcta atcgaagatg
     5341 gtccgaaagc cctgaaagag ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg
     5401 cgactcaggc gctgaacggt ttgattggcg ctggcgtacc gcaggactgg gcaacgcata
     5461 tgctgggcca cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg
     5521 tcctgcctgc actgtggaat gaaaaacgcg agaccaagcg cgctaagctg ctgcaatatg
     5581 ctgaacgcgt ctggaacatc actgaaggtt ccgacgatga gcgtattgac gccgcgattg
     5641 ccgcaacccg caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc
     5701 tggacggcag ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac
     5761 tgggcgaaaa tcatgacatt acgctggatg tcagccgccg tatatacgaa gccgcccgct
     5821 aagcttttta cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac
     5881 acctcaccgg agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac
     5941 cgttattaag ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc
     6001 aagtaatgag gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat
     6061 tgataccgcc gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc
     6121 agtcaacaga gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc
     6181 ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctactt
     6241 aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga
     6301 attgcaaaaa gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct
     6361 gcaacgcctg attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcatcc
     6421 gctgatgcaa caacgccagc tacacgcctg gaacgcgaca cacaaaatcc agaccgaatc
     6481 ctggagccca ttagcgcaag gagggaaagg cgttttcgat cagaaagtca ttcgcgatct
     6541 ggcagataaa tacggcaaaa ccccggcgca gattgttatc cgctggcatc tggatagcgg
     6601 cctggtggtg atcccgaaat cggtcacacc ttcacgtatt gccgaaaact ttgatgtctg
     6661 ggatttccgt ctcgacaaag acgaactcgg cgaaattgca aaactcgatc agggcaagcg
     6721 tctcggtccc gatcctgacc agttcggcgg ctaacatgca aattctcccg gtggcggtaa
     6781 tgttccgcta ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgttac
     6841 tcttccttgt tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta
     6901 aaagaacaac tcatctatat ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa
     6961 taatacttct gtttttagca gccctggcaa gttttaccgt acacgcacag cccccctcac
     7021 tgaccgtaga acaaacagtc cggcatattt atcagaacta taaatcagat gccactgccc
     7081 cttattttgg tgaaaccgga gagcgggcga taacttctgc gcgtattcaa caggcgctta
     7141 ccctgaacga caatcttacg ctgccgggca atattggctg gctggattat gatccggttt
     7201 gtgattgtca ggattttggc gatctggtgc tagaaagcgt tgcgataacc caaactgacg
     7261 ccgatcatgc cgatgccgtt gtgcgctttc gtatctttaa agatgataaa gaaaagacca
     7321 cgcagacact gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca
     7381 atcatggcag cgtcttacaa gcagttaata gcgagaatga aaaaacgctg gccgctttag
     7441 cttcgttgca aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg
     7501 attatagctg gccgtggacg tgggtggttt ccgactctta ccgccaggcg gttaatgcct
     7561 tctataaaac caccttcaag acggccaata atcccgatga agatatgcaa atagaacggc
     7621 aatttattta cgacaatccg atctgttttg gcgaagagtc gctattttca cgcgttgatg
     7681 aaattcgagt cctggagaaa accgccgatt ccgcccgcat tcatgttcgt tttacgctga
     7741 ccaatggcaa caacgaagag caagaactgg ttttacagcg gcgcgaaggc aagtgggaaa
     7801 tcgctgattt tatccgcccg aacagcggca gcctacttaa gcagattgag gcaaaaaccg
     7861 ccgccagatt aaagcaatga gctgaattaa ataacaatta gccggaacaa taaataaaag
     7921 ggaacactat atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct
     7981 cagtggctgc gtcaatgagc aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa
     8041 tgccaaaatc gcccggcttg agcaggatat gaaagcacta cgcccacaaa tctatgctgc
     8101 caaatccgaa gctaacagag ccaatacgcg tcttgatgct caggactatt ttgattgcct
     8161 gcgctgcttg cgtatgtacg cagaatgata aaaaaatccc cggcagcatg tcagttgccg
     8221 gggatttttt ttaacgtcca accgccgctt tagggcgttt cttcgcacca gcattcaccg
     8281 gacgagattg cgtagacgac gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca
     8341 tcggcgtatg tttcgtcaac gccggacggg tattgcggtt ctggcgacga gcttcacgca
     8401 tctcttcaat ggttggcgca ggcactaagc aatcgcgacg gctgccaatc agatgctttt
     8461 tgcccatcgc ttccagcgcc tggcggatta acggccagtt tgccggatcg tggtaacgca
     8521 acaacgcttt atgcaaacga cgctgtttgt cgcccttcgg tacgaagacg tcttcgctct
     8581 tataaccaat cttcgccagc gggtttttcc cggtgtaata catggtggtt gagttcgcca
     8641 gcggcgatgg gtagaagttc tgtacctggt cgagacggaa acgatgcttt ttcagccaca
     8701 gcgccagatt caccatatct tcatcacgcg taccggggtg cgcggagatg aaatagggga
     8761 tcagatactg ctctttacct gcctgttttg agtaagtatc gaacagctct ttaaagcggt
     8821 catagctgcc catgcccggc ttcatcatct tcgataacgg cccttcttcg gtatgttccg
     8881 gggcaatctt cagataaccg ccgacgtgat gggtcgccaa ctctttgata tagcgcggat
     8941 cttccacggc gatgtcataa cgtacgcctg aggcgatgag gattttttta atgcctttca
     9001 gatcacgcgc acggcgatag aggttgatcg tcggttcgtg gttagtgtcc atgtgcggac
     9061 aaatatccgg ataaacgcac gacaaacggc gacaagtttg ttcagcgcgt ggcgatttgc
     9121 agcgcaacat atacatgttg gcagttggcc caccgagatc ggaaatcacg cccgtaaaac
     9181 ctggaacggt gtcgcggatc gcttcgatct cattaatgat cgaatcttcc gaacggctct
     9241 gaataatgcg cccttcgtgc tcggtaatag aacagaaaga acagccgcca aagcagccgc
     9301 gcataatgtt gaccgaaaaa cggatcattt cgtaagccgg aatacgggca ttgccatagg
     9361 ccggatgtgg cacgcgcttg tacggcagcg caaaaacgct gtccatctct tcggtagaaa
     9421 gcgggatagc aggcgggttg atccacacat agcggtcacc gtgtttttgc atcaatgcgc
     9481 gggcacagcc tgggttggtt tcgtggtgca gaatgcgcga agcatgggcg tacagcactt
     9541 tatcgccctt cactttctcg aaagaaggca gcaacacgta ggttttttcc cacggtttcg
     9601 ggcgcggtgg ctgcacggtt acggctttgg cttcctgctt tttcggtgcc accggtttgt
     9661 tatccgcgca cggcaaatct tcaccatacg gatgcgggat tgggtcgatt tttccagggg
     9721 tatcaagacg ggtggaatcc acgccgctcc agccaggcag cgcctctttc acgataatcg
     9781 cggtattacg cacatcgcgg atttcactaa tcggctcgcc catcgccaga cggtgcgcca
     9841 cctccaccag cggacgctca ccgttaccaa acatcagcat gtcggctttc gaatccacca
     9901 gcacggaacg gcgcacggta tcggaccagt aatcataatg tgcggtacgg cgcagactag
     9961 cctcaatacc gccgaggatc accggtacat ctttccacgc ctctttacaa cgctgggtat
    10021 aaaccagtgt ggcgcgatcc gggcgcttac ccgcgacgtt atccggcgtg taggcatcgt
    10081 catgacgtaa acggcgatcg gcggtataac ggttgatcat cgaatccatg ttgccagcag
    10141 taacaccgaa aaacagattc ggtttaccca gacgcatgaa atcgtctttg ctgctccagt
    10201 ctggctgggc gatgatcccg acgcgaaagc cctgtgcttc cagcatacga ccgcaaatcg
    10261 ccatcccgaa gcttgggtga tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc
    10321 tatcccagcc aagttgatcc atctcttcac gagacatcgg caaaaacggt gccggtccaa
    10381 aacaggcggc ccagtactgc ggccaggaga acaggtcgcg atccggttgg atcagggaga
    10441 tagagctcat tttgcttcca gaaatgataa aaaaataatc aaaggccggg gattataagc
    10501 cggaacgaaa gagaaatcga aaggtattcc atactcgccc tcctcgggcg agtatgaaga
    10561 ttacggtacc ggattgacca aaagttgccc tatcgagcca cggtccgcca tttccagcgt
    10621 ctgactgttg aagtagaacg ggaagtgcgc ccaggaaggc tgaccgaaat aaacaagcag
    10681 ctccacttgt ccgtcaaccc aaacggtatc tttccagcct ctgtcttccg ggaacggcat
    10741 cgcgccattc acgttacgga tctggaacat tacgccttca atatggaacg cttgcggctc
    10801 gtccgcgcgt accgtccagc gttcccacgt tccttgctgc gcggtgacat caatacggtt
    10861 gacgtcccac agttgtccat taatacccgg gtcatcaccc agactgatat cgcgactgcg
    10921 aatcggcgaa ccggccatga tttcagttgg cagcaagcgc atcggaagac tgtctgtgac
    10981 cagcggcaga aggccggttg ggcgcagcgt tagcaccagg gtagaaacca gaatgctgga
    11041 tggctcaaag aagccacgaa tacgatcaac aatgctcgcc gcttcgccac aggtgatcga
    11101 cacttcatcg ccgttgctca tatccaccag aatctcgcgg cgctcgcccg gtgccagcga
    11161 aagttgcttc accgatacag gagcaggcag gaatccctga tcgccagaaa tcacatgtaa
    11221 cgggcgacca tcgctcatct gtagttgata acgacgagag ttcgacgcgt tcagcaggcg
    11281 caagcgcacc cagccacgcg agacttcaac gtacgggctt tgtacaccgt taaccagcag
    11341 cgtatcacca acaaagccgc cgcttcccgg ttcgttgtat tctggcgtac caaagttatc
    11401 cagccgttta tcctgaataa tgaccggaaa atcatccaca ccataatggt tggggatagg
    11461 cagcgacttg ctgacttcat cttccaccag ccacattccg gcaaggccgt tatagacctg
    11521 ctgagccgtg cggttgggag tattggcgtg ataccacaga gtagctgcgt tctggcgaat
    11581 aggcagtacg ggtgcccagt cagcgtttgg cgacatcatc cgtgccggac cgcccatcag
    11641 cgggcctggt acctgtagcc cggctaccgt cattgagaca ttttctgtca ggcggttgct
    11701 gtaaataagc ttaacatcgt cgcccttcca gacgcggata gtcggcccca ggtaacgacc
    11761 attgattccc cagaccgacg cgcgtgtccc tggcgtaaat gaccagtgcg cacgttgtac
    11821 agtcataaac agcggttgcc cacggcgaga ttcaagcagc ggcggaacgg gtagcggttg
    11881 ctgttgcccg gctgcgctgg ccttcagggg aacagcgcct gcacaaagtg caatccccga
    11941 tgcctgaatg aactgacgcc gactgagtga catatttgct ccatgtaaaa ctggctaaag
    12001 taataccgtg caggaaagtg ttccccgcaa aaatacagat tgccgactta aacttttccg
    12061 gcggcttcgc gttctgcgac ttctttatcg agctcggcga ttttttgttc cattatcgaa
    12121 cgacaatggg cagccagctc acgaacctga tctttgccat actgactgac gtcaattggc
    12181 ggcagcattt cgacaatcac cagaccgttg tgcagtcgat taagattaat cttattcgaa
    12241 gttgtagaga cgcacacggg aataatcggg acgcccgccg caattgccgc gtgaaatgct
    12301 ccagtcttga acggtagcag gccgcgacca cggctgcggg ttccttccgg gaacatccag
    12361 atggaaatac ggcgtttttt gaaatgattc acgacttccg caatggtgcc gtgagcttta
    12421 gtgcgattgt ttctgtcgat caataagttg ccggttaacc agtacaactg cccgaagaag
    12481 gggatccaca gcaagctctt tttacctacc gtcaccgtcg gcggttgcac aatgttcgat
    12541 gctgtcacca tgtcatagtt gttctggtgg ttagcgatat agatagcatt gccgtagctt
    12601 tcagcatctg caggtttgcg gcactcaact ttcaggccga acagcggcgc aagacggcca
    12661 aacatatgcc caaaggtggc cacatgtttc gggttacgcg ggctgaaaag gcagtaaatg
    12721 gagccgaata cacagactaa gatgctgtaa atcacggtaa taataagacg aaagatatat
    12781 agcataacac cctctgaagg tctgagagac tggcattgta cgtcacctga aatcaggtta
    12841 gggcattgtt aaaagccgct ctctggaaaa gcgtattgtt aatcgcaacg catgaataaa
    12901 caacggtttc aagggaattt ttatcgtttc cgggggttga atacgctgcc ggatgacgac
    12961 ttaacgtttc atccggcgtt ccttgcaagc gggaggaaac agcgccctcc ccggcatatt
    13021 actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca cgatcgatac
    13081 gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact ttctgtagct
    13141 cttccggacg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg ctttgcggcg
    13201 gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc gatggaatgt
    13261 tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc gggaacatca
    13321 acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca atcaccaccg
    13381 gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca cggttacgcg
    13441 ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc atcagcagtt
    13501 tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc aacgttaatt
    13561 tgccggtgag cggctcgccc tgaccacgcg ccgacggcag cgtaatcggg tcaatggcat
    13621 agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct ttcaccgccg
    13681 ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca tggcctttag
    13741 cgctgcgtac ccagcccatc tgcgacagca caatggtgac aggttcagac ggcagcatgt
    13801 cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga cgatcgtcac
    13861 cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt ttacgctcgg
    13921 aagccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc tcaccgcgaa
    13981 tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc gcttccgcct
    14041 gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct tcgttacgaa
    14101 tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc aggatatgca
    14161 ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta tcgcgacgga
    14221 acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga ccatccagac
    14281 cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg aagaggtggt
    14341 tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga cgggtcgggt
    14401 tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg ttgcgcattt
    14461 gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggtaat gcgctgataa
    14521 ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca cgtccgttct
    14581 cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc ggataatccg
    14641 gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg attaatgcga
    14701 ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc atgccgacgg
    14761 caataccggt ggtgccgtta agcaaaatgt ttggcagacg agcaggcagc attttcggct
    14821 cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc agctcgctca
    14881 atagcagctc ggaatatttc gacaaccggg attcggtgta acgcattgcc gcgaacgatt
    14941 tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa cggtaagaga
    15001 acggctgcgc catcaggacc atcgcttcat aacaggcgct atcgccgtgc ggatggtatt
    15061 tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg gcattcaggc
    15121 ccagttcaga catcgcatac acaatgcggc gctgaacagg tttcagacca tcaccaataa
    15181 acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg ttttccgtaa
    15241 attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc tcaacttatt
    15301 cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg cgtcgagtca
    15361 caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa ggcatcaatg
    15421 tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag cacaaggttg
    15481 cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga agttgaggtt
    15541 atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata cggttcagcc
    15601 cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg gcttgctcat
    15661 ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat acatggtatt
    15721 ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt cgctcaaact
    15781 tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcca acggcacata
    15841 aacatccgtt tccagtcgct gctctgtcac cccttcggca agattggtgt agtgaaacag
    15901 aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa taatgcccca
    15961 tatcgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat aacgtccacc
    16021 ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc taacgctgcc
    16081 gcagatatca aagcgaaacg cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc
    16141 aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca tgataaattt
    16201 cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg ccacagggat
    16261 tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc ctccctgcga
    16321 aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact ttgcggatct
    16381 gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttcaccctga atctcaaccg
    16441 tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca acattgatag
    16501 taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg atattgccgc
    16561 gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacggtc gttacgctgc
    16621 cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc
    16681 cctgctctgc tgccatcacc ggtgcgctgc acagggccat tactgcgatt actgctgcga
    16741 attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac aagatagtcc
    16801 ttaacaactt cttaagggaa aaaaataaaa tttagtgctg tacagagcgc gttacaacac
    16861 ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt ttactgatag
    16921 aagatgacat gctgattggc gacggcatca aaacgggcct tagtaaaatg ggttttagcg
    16981 tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgcg ccttatgatg
    17041 cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg cgcgaatggc
    17101 gagaaaaagg tcagcgtgag ccggtactga tcctgaccgc gcgcgatgcg ctggaggaac
    17161 gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt gcgttgatag
    17221 aagtcgccgc caggctggaa gctctgatgc gccgaaccaa cggccaggcc agcaacgagc
    17281 tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac
    17341 ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt aacgctggtc
    17401 gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa gaggtcacca
    17461 gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt gattttattc
    17521 gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc aacgtcttag
    17581 tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct ggctgctttc
    17641 cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg acacccaact
    17701 gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg cggcggatcg
    17761 catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg cgctgacctt
    17821 tgccatcttt acccacgacg gcagaatggt ccttaacgat ggcgataacg gagaagatat
    17881 tccttatagc tatcaacggg aaggttttgc tgacgggcaa ctggtgggcg ataaagatca
    17941 atggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg ttggtcagga
    18001 atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga tcccgtggct
    18061 ggtcgcactg ccgattatgt taatcatcat gatggtacta ttgggtcgtg aactcgcgcc
    18121 gctgaacaaa ctggcgctgg cgctgcgtat gcgtgaccct gactcggaaa aaccactaaa
    18181 cgcgactggc gtacccagcg aagtgcgtcc gttggttgag tcgctaaatc aactgttcgc
    18241 ccgcacacat gcgatgatgg ttcgtgaacg acgctttacc tccgacgcag ctcacgaact
    18301 acgtagcccg ttaacggcgc tgaaagtgca aaccgaagtt gcgcagcttt ctgacgatga
    18361 tccgcaagcg cggaaaaaag cgctgcttca attacattac gggatcgatc gcgctactcg
    18421 tctggttgat caactgctca ctctatcgcg gctggactca ctggataacc ttcaggacgt
    18481 cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt accacacggc
    18541 gcagcaggcg aaaattgacg tgcgactgac actcaatgcc cacggcatca aacgcaccgg
    18601 acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg tgcgctacag
    18661 tccacagggc agcgtgatag acgtcacgct gaatgctgat aatttcatcg tgagggataa
    18721 cggccccggt gtgacgccag aggctctggc gcgaattggc gaacgcttct atcgcccccc
    18781 cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaaatt
    18841 gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag
    18901 ctggtaactt ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc
    18961 aaaatataaa ccgtccccaa aaaagccacc aaccacaaac cagacaaata ccgagcgaaa
    19021 atacatatac ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa
    19081 taagtggcgt aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg
    19141 aaacggaagg tatactgcaa atattgtctc ccgactcaag catccagtca tacttattga
    19201 aagcaaaaaa agcatctatt aaccaataag taagcattat gtataacaga gcttcaaata
    19261 ttgtttgaat tttatgcttt aacatatatc agtacgctca taaaaatcca tttataatta
    19321 tcaacatgac acgttgatat tttcatcttt tacttattca aagccatatt ttcaatccca
    19381 aaaaaaaccg aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc
    19441 tatcagtatt gtcattttca gaaaatattt atcctgcatc ggtgagtcag agcaagatca
    19501 gacttttgct aaattcgcaa aagactttgc acattttgct aatttcaccg taccgctctg
    19561 tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaatgagca acatcctgat
    19621 tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga
    19681 agtcgctgat ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag
    19741 cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca
    19801 gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt
    19861 caccgaaggt catggaacgc tgtatgccag cgatggccgt acccgcaaag atccgtcaaa
    19921 aaaatacggt tccggcggcc tggtacaggg caaaaaatat atgctttctc tgacctggaa
    19981 cgcaccaatg gaagccttca ccgaaaaaga tcaattcttc cacggcgttg gcgtcgacgg
    20041 tgtgtatctg ccgttccata aagcaaacca attccttggc atggaaccgc tgccgacatt
    20101 tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa
    20161 gcatcttgtg gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct
    20221 taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga
    20281 tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc
    20341 aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat
    20401 gattgagcag tgggaaagca tcgcgcatct tgaagcgcat ctgcaaaccc cgcacatgaa
    20461 ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg aatatccgca tcctgcaacc
    20521 tgggatttaa acctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta
    20581 aacctcaatc tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg
    20641 tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat
    20701 agtcaactgc accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg
    20761 gttcatttcc cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa
    20821 ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag
    20881 atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg
    20941 gaagtgtttt acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc
    21001 atccgcgagg atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc
    21061 gataccgatc gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga
    21121 aacttcccag gtgttaagga tcttaccttt cagtggcatg atcgcctgat attcgcgatc
    21181 gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc acaaggaaca gttcggtacg
    21241 gttaagatcc tgcgcggtac agtcagccag tttgccaggc agtgctgggc cgctggtcag
    21301 ctttttgcgc accacttttt tagccgcacg catacggcgc tgggcgctgg aaatcgccat
    21361 ctccgccagc agctccgccg cctgaacgtt ctggttcagc cacaggataa aggcatcttt
    21421 caccacgcct gaaacgaatg ccgcgcattg acgcgaagag agacgctctt tggtctgccc
    21481 ggcaaactgc ggatcctgca ttttcaccga cagaacatag gcgcagcgat cccagatatc
    21541 ttccgccgac agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat
    21601 cgcgtccaac aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat
    21661 aaggttgacg tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca
    21721 gtccacagct tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg
    21781 taaaccgttt accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg
    21841 ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt
    21901 ggccttcagc acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc
    21961 atccggccag aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg
    22021 taaatcctgc accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg
    22081 cacgttaact tctacgcgct tcgacagggc gttaaccacc gaaatcccca cgccatgcag
    22141 gccgccagag aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag
    22201 aatcagttca accgccggta caccctcttc cgggtgaata tccaccggca tcccgcgccc
    22261 atcgtcaata acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg
    22321 acccgccagt gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg
    22381 agtggtatcg gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc
    22441 aatggcatca gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc
    22501 agagatggtg cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga
    22561 atgcgtggtt gccgccttct atgacagtct ggcggcagga agcgtagtac gccaccgcct
    22621 ggcggtaatc cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt
    22681 ccagcgggtc aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt
    22741 gctgcccggt gtaggggttc tcgttctgac cgagatagtc cgtcagcagt tcaaacgggc
    22801 gtaccgccgg gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt
    22861 aatatccccc cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa
    22921 tggattccaa cagctctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct
    22981 caacgtcagg gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg
    23041 gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt
    23101 atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc
    23161 atgtaactcg agagtacgcc agccgggcgc gatggtgtcc agcgtaaagt tggaacagtg
    23221 cggcttaaac tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag
    23281 atccagctcc tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag
    23341 cacggtatcc agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc
    23401 aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt
    23461 acgttccagc cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg
    23521 gctatccagc aacaggattt gccattgctc accaataaac acgcgcttcg ccggggagat
    23581 acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca
    23641 gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga
    23701 ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct gatgtggacg
    23761 aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg
    23821 cttttgtgca aacaggtgag tatcggtaat ttgtaaaatc ctgactctgg cctcaccagc
    23881 cagaggaagg gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac
    23941 cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt
    24001 aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt
    24061 gaagcgaaaa atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg
    24121 caccgtcatt gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac
    24181 cagggtagtg tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg
    24241 atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa
    24301 gttcatctcg cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc
    24361 ccactcattt tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc
    24421 tgcgttgtcg attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg
    24481 aacgcgaata tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc
    24541 cacttcgccc accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact
    24601 taacaccggt ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc
    24661 aacatcttcc acactttcac cctcttctat catcccggca accatctcca gtagccaggg
    24721 ggtttcactg gtgtcgtatg cggcaatccg aatctgctca atcagcacaa cttcatcacg
    24781 cactgggtca aagggtagca agactgcggc gtgaccgcgc tcaaaaattt ccctccgtac
    24841 ctcatgactc atttgcccgt tgaataaacg atgacgaaat ctataaagat ctaatgaaaa
    24901 aaagccgcga taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac
    24961 gggcaggttg tctggcttaa gcattgttaa tgtcctggca ctaatagtga attaaatgtg
    25021 aatttcagcg acgtttgact gccgtttgag cagtcatgtg ttaaattgag gcacattaac
    25081 gccctatggc acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa
    25141 ttttacagtt tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg
    25201 ctccccattc ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac
    25261 ctgatgcaag tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc
    25321 gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta
    25381 ggtttaggtg cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactct
    25441 aacgcgacca gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt
    25501 gcgttaacgc tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag
    25561 caaaccttga tcctcaacac cgcgaccgct tattttaacg tgttgaatgc tattgacgtt
    25621 ctttcctata cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa
    25681 cgttttaacg tgggcctggt agcgataacc gacgtgcaga acgcccgcgc gcagtacgat
    25741 accgtgctgg cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg
    25801 cgccagatca ccggtaacta ctatccagaa ctggcagcgc tgaatgtcga aaactttaaa
    25861 accgacaaac cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg
    25921 ctgttacagg cacgcttgag ccaggacctg gcgcgcgagc aaattcgcca ggcgcaggat
    25981 ggtcacttac cgacgctgga tttaacggct tctaccggga tttctgacac ctcttatagc
    26041 ggttcgaaaa ctcgtggtgc cgctggtacc cagtatgacg acagcaatat gggccagaac
    26101 aaagtgggcc tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg
    26161 aaacaggccc agtacaactt tgttggtgcc agcgagcaac tggaaagcgc gcatcgtagc
    26221 gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg catctatcag tagcattaac
    26281 gcctacaaac aagccgtagt ttccgctcaa agctcattag acgcgatgga agcgggctac
    26341 tcggtcggta cgcgtaccat tgttgatgtg ttggatgcaa ccaccacgct gtacaacgct
    26401 aagcaagagc tggcaaatgc gcgttataac tacctgatta atcagctgaa tattaagtca
    26461 gccctgggta cgttgaacga gcaggatctg ctggcactga acaatgcgct gagcaaaccg
    26521 gtttccacta atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat
    26581 ggttatgcgc ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc
    26641 agtaacggtc ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga
    26701 acgtaaggca acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat
    26761 ttcgaccagc catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg
    26821 aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa actggagcgc gcgccatctg
    26881 acaccagtcg ctctcgcggt tgccactgtt tttatgctgg ctggctgtga aaagagtgat
    26941 gaaacagtgt ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc
    27001 gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa
    27061 tacgccaccc gtgaagactg tgttgctgaa tttggtgaag gtcagtgcca gcaggcacca
    27121 gcccaggctg gcatggcacc agaaaaccag gcgcaggccc agcaatccag cgggagtttc
    27181 tggatgccgc tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt
    27241 gcacagcagc cgctgttctc ctcgaaaaac ccagccagcc cggcttacgg taaatatacc
    27301 gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag
    27361 acagccatgg caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa
    27421 tctgttgcca aacaaagcac tatgcagcgt agcgcaactg gcacctcttc tcgttcaatg
    27481 ggtggctgat accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag
    27541 cccacgaata cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg
    27601 cttactacaa gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc
    27661 accagatgtg cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat
    27721 tccgcattcc aaaacacacc tggagttttg tgcgccagtc atggctgacg caccagccat
    27781 cgctttattc gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa
    27841 ataacgccga tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg
    27901 aagatcagct taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag
    27961 aaaaactgat cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc
    28021 tcacctgctg tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg
    28081 caacggaagc tgaaattgct actgagttcc tctacatcga tgatatcggg ttaggtgaaa
    28141 aaggtcagtt cacggattta caggatcagg taatttccaa cctgttcaag ctgtatccgt
    28201 gggaatttat gttgcgtgag atgttttcca ccaagctgga ggatgcaggc gtacgctggc
    28261 tggaaccggc gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga
    28321 tgttcccgaa tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa
    28381 tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg
    28441 aaaacggcaa aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc
    28501 agcaattcca cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg
    28561 tgaacgatca acccgccgga attggcattc gtgaagaccg tgcattgatc acccaggata
    28621 tgtctcggtt ttatccacat atttttgttg agtaatcaat gatgccggat ggcattcgcc
    28681 atccggtaat tgttaaccta tctgcaccga cagcatactc aggctgccca tttctatacc
    28741 atcgaccgga atggtaattg gctcctgccc atcccacgca cctaagacat acaacaacgg
    28801 cagatagtgc tccggcgttg ggttcgataa cgcgccacct tcatggtcga ggtaattcac
    28861 cagaggatgc tgctctaccg ccccttgcca cgtcagattc gctttcacat actcattaaa
    28921 cgacatcgcc caaggatatg gtgaactatc accgtgccac ttcactgtac gcaggttatg
    28981 caccacgttg ccactggcga ccaacattat tccttcatct cgcagcgctg ccagtttgcg
    29041 ccccatttcg aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatcgg
    29101 gatatcggcg tcaggataca tcttaatcag cacgccccag gaaccgtggt caaagcccca
    29161 ggcttcttta tccagcgtca ccgggaccgg cgctaacagc tcaaccagat gctgtgccag
    29221 cacaggcgaa cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa
    29281 gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg
    29341 agccgaaacc accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct
    29401 gcgggtatac aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc
    29461 tggcatacgt gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc
    29521 acaatacgct tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat
    29581 tttgacgatc tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat
    29641 tctgaccata ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcgggca
    29701 aaaaccctcg aaccgcttac tggcgttttc gctaggattt gcggcgggga tcatgttgct
    29761 catctcatta atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgcctgtgtt
    29821 gggttatgga atgtttatct tcggtctgct tggctatttt ggcctggacc gcatgttgcc
    29881 acatgcgcat ccgcaggatt taatgcaaaa atcggtgcag ccgttgccaa aatcgatcaa
    29941 gcgcacagcc attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc
    30001 cacctttgtc acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc
    30061 tttgcacaat atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc
    30121 taaacgcacc gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt
    30181 gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc
    30241 ggcggttgca ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga
    30301 gattgaccct aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt
    30361 cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag
    30421 cacatggaca acttctgcca gcttcacgaa agccttattc taccttttcg gctgtctctt
    30481 ctctcgtact gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa
    30541 acgcccgaca ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata
    30601 caatccagta ccaggcgata aacatccttt t
//
LOCUS       DAPHDX010000047        30183 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPHDX010000047 DAPHDX010000000
VERSION     DAPHDX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.047"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            127..1710
                     /locus_tag="RH058_002059"
     CDS             127..1710
                     /locus_tag="RH058_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV3137649.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1984..3837)
                     /gene="asmA"
                     /locus_tag="RH058_002060"
     CDS             complement(1984..3837)
                     /gene="asmA"
                     /locus_tag="RH058_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV3137650.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVKMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3859..4440)
                     /gene="dcd"
                     /locus_tag="RH058_002061"
     CDS             complement(3859..4440)
                     /gene="dcd"
                     /locus_tag="RH058_002061"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV3137651.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4532..5173)
                     /gene="udk"
                     /locus_tag="RH058_002062"
     CDS             complement(4532..5173)
                     /gene="udk"
                     /locus_tag="RH058_002062"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV3137652.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5491..8808
                     /locus_tag="RH058_002063"
     CDS             5491..8808
                     /locus_tag="RH058_002063"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV3137653.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8847..9704)
                     /gene="alkA"
                     /locus_tag="RH058_002064"
     CDS             complement(8847..9704)
                     /gene="alkA"
                     /locus_tag="RH058_002064"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV3137654.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9838..11190
                     /gene="yegD"
                     /locus_tag="RH058_002065"
     CDS             9838..11190
                     /gene="yegD"
                     /locus_tag="RH058_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3137655.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11202..13142)
                     /gene="yegI"
                     /locus_tag="RH058_002066"
     CDS             complement(11202..13142)
                     /gene="yegI"
                     /locus_tag="RH058_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV3137656.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYN
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13139..13900)
                     /gene="pphC"
                     /locus_tag="RH058_002067"
     CDS             complement(13139..13900)
                     /gene="pphC"
                     /locus_tag="RH058_002067"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDV3137657.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13897..14556)
                     /locus_tag="RH058_002068"
     CDS             complement(13897..14556)
                     /locus_tag="RH058_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV3137658.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VAFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14777..14836)
                     /gene="ibsA"
                     /locus_tag="RH058_002069"
     CDS             complement(14777..14836)
                     /gene="ibsA"
                     /locus_tag="RH058_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV3137659.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15109..15165)
                     /locus_tag="RH058_002070"
     CDS             complement(15109..15165)
                     /locus_tag="RH058_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3137660.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15437..15493)
                     /locus_tag="RH058_002071"
     CDS             complement(15437..15493)
                     /locus_tag="RH058_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3137661.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15772..17019
                     /gene="mdtA"
                     /locus_tag="RH058_002072"
     CDS             15772..17019
                     /gene="mdtA"
                     /locus_tag="RH058_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV3137662.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17019..20141
                     /gene="mdtB"
                     /locus_tag="RH058_002073"
     CDS             17019..20141
                     /gene="mdtB"
                     /locus_tag="RH058_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV3137663.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20142..23219
                     /gene="mdtC"
                     /locus_tag="RH058_002074"
     CDS             20142..23219
                     /gene="mdtC"
                     /locus_tag="RH058_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV3137664.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23220..24635
                     /locus_tag="RH058_002075"
     CDS             23220..24635
                     /locus_tag="RH058_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137665.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIDGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24632..26035
                     /gene="baeS"
                     /locus_tag="RH058_002076"
     CDS             24632..26035
                     /gene="baeS"
                     /locus_tag="RH058_002076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV3137666.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26032..26754
                     /gene="baeR"
                     /locus_tag="RH058_002077"
     CDS             26032..26754
                     /gene="baeR"
                     /locus_tag="RH058_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV3137667.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGIDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26945..27277
                     /locus_tag="RH058_002078"
     CDS             26945..27277
                     /locus_tag="RH058_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV3137668.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27486..27782
                     /locus_tag="RH058_002079"
     CDS             27486..27782
                     /locus_tag="RH058_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV3137669.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27784..28080
                     /locus_tag="RH058_002080"
     CDS             27784..28080
                     /locus_tag="RH058_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3137670.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28183..29544
                     /gene="yegQ"
                     /locus_tag="RH058_002081"
     CDS             28183..29544
                     /gene="yegQ"
                     /locus_tag="RH058_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV3137671.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29874..30182)
                     /locus_tag="RH058_002082"
     CDS             complement(29874..30182)
                     /locus_tag="RH058_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137672.1"
                     /translation="MAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDNQYY
                     TEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE
                     "
BASE COUNT         6983 a         8039 c         7973 g         7188 t
ORIGIN      
        1 ataatgtgag gttattaccc tcaaataata tgagataaat agtgctgcaa cattgcattt
       61 ttgccccgat ttatccatga tcgaattgtg acatttgtca tacaacgaat aggttttgta
      121 cttactatgg aatggattgc cgatccgtct atctgggccg ggttaatcac gctgattgtg
      181 atcgaactgg tcctcggcat tgataacctg gtctttattg ccatcctcgc cgaaaaacta
      241 ccgccgaagc agcgcgaccg cgcacgggtt accgggctgc tgctggcgat gttaatgcgc
      301 ctgttactgc tggcgtcaat ctcctggctg gtcaccctga ctcaaccgct gttcagcttc
      361 cgctcgttta cctttagcgc ccgcgactta atcatgctgt ttggtggttt cttcctgttg
      421 ttcaaagcca cgatggagct gaacgaacgg ctggaaggga aagacagcaa taatcccaca
      481 caacgcaaag gtgcgaagtt ctggggcgtg gtgacgcaaa ttgtggtact ggacgccatt
      541 ttctcactcg actcagtgat taccgccgtc gggatggtcg accatttact ggtcatgatg
      601 gccgccgtgg ttatcgctat cagcctgatg ttgatggcca gcaagccgtt aacgcaattt
      661 gttaacagtc acccgacgat cgttattctc tgcttaagct tcctgttgat gattggcttt
      721 agcctggtgg cagaaggttt cggcttcgtc attccgaaag gctacctgta cgctgccatt
      781 ggtttctcgg tgatgatcga ggcgctcaat cagttggcta tctttaaccg gcgacgtttt
      841 ctttccgcta accagacgct gcgccagcgg actaccgaag cggtaatgcg cctgcttagc
      901 gggcaaaaag aagatgcgga actggacgcc gaaaccgcgt ccatgctggt ggatcatggt
      961 aaccagcaga tctttaatcc gcaggaacgg cggatgattg agcgggtact taatcttaac
     1021 cagcgtaccg tcagcagcat tatgacgtcg cgccacgata ttgagcatat cgatctcaac
     1081 gcgccggaag acgagatccg ccagttactg gagcgaaatc agcatacgcg gctggtggtt
     1141 accgacggtg atgatgcaga agatttgctc ggtgttgttc acgttatcga cctgctacaa
     1201 cagtcactgc gcggcgaacc gctcaacctg cgggtgttga ttcgccagcc gctggtgttc
     1261 ccggaaacct tgccgttgtt acctgccctg gagcagttcc gtaatgcccg cacgcacttt
     1321 gcttttgtgg tggatgagtt tggctcggtg gaagggattg tgacattaag tgacgtcact
     1381 gaaaccattg ccggtaactt accgaacgaa gtggaagaga tcgacgcccg ccatgatatt
     1441 cagaagaatg cggacggttc ctggacggcg aatggtcata tgccgctgga agatctggtg
     1501 caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc acaccattgc cgggctgttg
     1561 atggagtatt tgcaacgtat tccaaaacct ggcgaagaag ttcaggtggg ggattatttg
     1621 cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg tgcagattat tccactgcgt
     1681 aaggatggcg agatggagta cgaggtgtga tggtatcggt cattttaccc gatagccgaa
     1741 aggaataaga tgagggaacc gcgccagcgg cgatttaccg ccgggagccc gggttgccag
     1801 ggtggtggcg attgagccac cctggcacgt tcaccggctt gagcgttcca tagtagtgag
     1861 gaacatcaag tgaacggaac agccttcgat cttacatatt cagaatataa tggtgccgcc
     1921 gcgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc tacaggacag gtcacctggt
     1981 aacctacatc ttctccagca acttcttcac atctttgcca ttgcgggaat ctttattccg
     2041 ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt agatgtttgc gcagtaactg
     2101 atccacttgc agactgtaat tgagttgctg ccagttgcca taaacccgca gcggtactgg
     2161 cgtttctttg aggaaatcaa tcagtttgct ttccccgttc cagccaccca cgacccgaat
     2221 atcaaactgg gtgtcgcagg tttgatctgc cagattcaac atgccttccc ctgtcagcgc
     2281 cagcacaggc gattgacctt gcatgtcgtt taacgtcacg acgccatcct tcaacgtcaa
     2341 atcggtggta aagcggtcaa gccgcgtcac gttatcaaag ttttcagcgg ccttcacatc
     2401 accgccatta cgctctaccg cttgctgaat catctgctgg aagttcatcc cttccatgcg
     2461 cgtgtcggtc attttgacat gtgcctgtcc ttgccagttg tggcggaatg cgtcggcatc
     2521 tatgtcagca ccggagaaat caccagccag tgacattttt ccggtcaacg aaatcggata
     2581 gttaaacgcc ttcagaatgg taccaatctc aacgttttcc agccgtggct ggaagtttat
     2641 ccgcggattt attgatgtcg cgtccagcgt gcccggcagt gaaacctgtc caccgttaag
     2701 cttgccctgc agttgagtaa tttccagcaa acccgacttg ttggtcattt gcgtggcaac
     2761 atctgtaaaa ttcattccgc gccagcgcac gttactggcc tgcaacaaaa tatcagccgt
     2821 aaagccttgc agtccctgat atgccggttc atcaatacgc gaagaaatga ccgggcgcgg
     2881 caatgttgat tggctctgcc cctgctgcgc ggcaccgttt tcaccattcg ctgtttcatt
     2941 aagcgggatg aggttgtcaa gattcagttg cgggaattgc agtctcagct gccattcagg
     3001 tttctctgtc agcgtgacct gtgcttgccc gctcagcgtg ctgtcattgg cggtcaagct
     3061 gatttggtta aatgaaaggc gtttatgtga ctcctgccac tggacctgga aactcccctg
     3121 cccctgaata ccttgttttg gtaaatctgc accctgcaac tgccagttaa tttgttcaat
     3181 agccgccgtt aaatcatgcg gataatcaga agcatctacc gtaccgttaa gggatatcgt
     3241 gagatcgcgc tgatcgcgat taacccgccc ggagaactca aatgagccac gatgttgggg
     3301 atcttgttcc atttgcaggc ggatattgcg gattgtcacc tgctcgtcat cttcatgctg
     3361 gaacaccagc acgctgtccg ccaccttaag actggatata tcaaacgacc atccgcgatc
     3421 gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac
     3481 cgcttccgtc tgcggcgtca gttggatcac tgcccctttt agcatcacct gcttaacgct
     3541 cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga cgcatgttgt cggcgcgaac
     3601 cagtggctgg cttgcgccct gggcggtgag agacattcgc ccggagagga tactaagctg
     3661 cggccagacg tgccaacgca gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc
     3721 aacttgcttg accatatagt cgcggaaatc attcggattc accagcaaca ctaacgcaga
     3781 taacccggcc accagcacga ccaggagtat catcagcgtc gtcagaaatc gtctcatggt
     3841 atcctcaatg ggcctgaatt agtctttatc gattcggctg gctaccgcgc cctgctggtt
     3901 gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc
     3961 aaagctcagc gcaccaatta acatgcccgg acgcagcgcc agcggcagct taccggagtt
     4021 gtagaactcc agcacaatgc aaccagacca gcccggatcg atacggtgcg cggtgacgtg
     4081 caccatcagc cccagacgcg ccaaagagga acgcccgtcc agccagccca ccagatcggc
     4141 tggcagcgtc accgactcca gcgtcaccgc cagcgccagc tctcctgggt gaagatagaa
     4201 cgcctcgccc tcgtcgagaa cgatctcatc gctcatcacg cggtcaagcg cggcgctcac
     4261 ttcatctttg ggaccgctca gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa
     4321 tttattgccc aggcgtacat ccaccgtcgc gccgttaata cgctccactg gcggacgtgg
     4381 gttgatcgac aaacggccct catcaagcca ggcttcaata tctcggtcac acagacgcat
     4441 ggcattttct cctttcgcgc atcactccct taacgccaat tacgtcaagg gcataccagg
     4501 ttatcactga acggtacaca atttatcaag cttattcaaa gaactgactt attttcgctt
     4561 tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg tccgcatatt
     4621 gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga tattgcgcca
     4681 tcactgaatc cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc
     4741 agatatccag cggggtatca acgaaaatgg agaagttaag ttcgtcacgc aaacgcgcat
     4801 ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt cggctcaacc gtcaccgttt
     4861 ctttcatacg cgtatgttca acatagctgt aaaccggcag gtcaattgcc gagccgcgtt
     4921 tcaacgcttg taaatgctca agcaacaggc tgtgatccat cgcgctggga tggtcgtagt
     4981 tggtcttaac gcgttcttcc atcgacagat ggctttgatc tttgtaatag cagtcttcgg
     5041 gaattacgcc gatgtgttca tcaccgactt gctcacgtaa ttcacgataa agggtactgg
     5101 caataagact cttgccggaa gccgatgcgc cagcgatacc gataatgacg cactgatgag
     5161 actgatcagt catatattta gcgacctgat taacctggat gttaggaagg gggcgacgaa
     5221 gcgccaaacg cggcaattat agggatttca tccgcctgat accagtcgaa tagcgctgcc
     5281 gcgcgctcag agttaattgt tgacgaagaa ttcccggtgg caaattacgt tgatcagttt
     5341 tatacaaggt aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta cgtcacttta
     5401 tgagcaattc gcatataaaa tgtaaaactt ttgtactagc ataaacacag aaacgaatac
     5461 tggcgacctg gtcttgcgga taaagcggta atgagcaaac aatcacagca tgtattaatt
     5521 gccctgcccc acccgctgct tcacctggtc agtttaggtt tagtctcgtt tatctttacc
     5581 cttttctcgc ttgagctttc gcagtttggc acccaactcg ccccactgtg gttcccgacg
     5641 tccatcatga tggtggcgtt ttatcgccat gccgggcgca tgtggccggg aattgcgctg
     5701 agctgctcgc tgggaaatat cgccgcatcc atcctgcttt tttccaccag ctcgctgaac
     5761 atgacctgga cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct gctacgtaaa
     5821 ttgctgccgt ggtataaccc cttgcaaaat ctggctgact ggctgcgtct ggcattcggc
     5881 agcgccattg ttccgcctct gctagggggt gttctggttg tcctgctgac gcccggagac
     5941 gatcctctca gggcattttt gatatgggta ctgtcagaat ccatcggcgc tctggcactg
     6001 gtgccgctgg gattgttatt taaaccacac tatctgctgc gccatcgcaa cccacggttg
     6061 ctttttgagt cgctgctcac gttagccatc acactgacgt taagctggct ttcgatgctg
     6121 tatctgccgt ggccttttac tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg
     6181 ccacgaatgg aagccttttt gatcttcctt accacggtga tgatggtgtc gctgatgatg
     6241 gccgcggatc cctccctgct tgctacgccg cgtacatacc tgatgagcca tatgccgtgg
     6301 ctaccgtttt tgctgatcct gctgcccgcc aacatcatga cgatggtgat gtatgccttt
     6361 cgtgcggaac gcaaacacat ttccgaaagc gaaacccgtt ttcggaacgc catggaatat
     6421 tccgctatcg gtatggcgtt agtgggcacc gagggacaat ggctgcaaac caacaaagcg
     6481 ctctgccagt ttctcgggta cagtcaggaa gagctgcgcg gactcacctt tcagcaactg
     6541 acctggccgg aggatctcaa taaagatctc caacaggttg aaaagctgat aagcggtgaa
     6601 ataaacacct attcaatgga aaaacgctac tacaaccgca atggcgatgt tgtctgggcg
     6661 ttgcttgccg tctcactggt gcgccacacg gatggcacgc cgctctattt tatcgctcag
     6721 attgaagaca ttaacgagct aaaacgcacc gaacaggtga atcagcaact gatggagcgc
     6781 atcacgctgg ctaacgaagc gggcgggatt ggcatctggg agtgggagtt gaagccgaat
     6841 atttttagct gggataagcg gatgttcgag ctgtatgaaa ttcctccaca tatcaaaccg
     6901 aactggcagg tgtggtacga gtgcgtgctg ccggaagatc gccagcatgc cgaaaaagtg
     6961 attcgtgatt cgttgcaatc acgctcgccc tttaaactgg aatttcgcat taccgtaaaa
     7021 gacggtattc gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc
     7081 gaacgcctgc tcggcattaa tatggatatg actgaggtta aacagcttaa cgaggcattg
     7141 tttcaggaaa aagagcgcct gcacataacg ctggactcca tcggcgaagc cgtggtctgt
     7201 attgatatgg caatgaaaat tacctttatg aatccagtgg cggagaagat gagcggctgg
     7261 acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac ctttggcgac
     7321 aatggtcccc tgatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa
     7381 gatgtggtgt tgcactgccg gagcggcggc agctacgacg tgcattacag tattacgccg
     7441 ttaagtactc tggacggcag caatattggt tcggttctgg tgattcagga cgttaccgaa
     7501 tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc
     7561 gccaatcgcg ccagttttga gaagcaactg cgtatgctgc tgcaaacggt aaacagtaca
     7621 catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc
     7681 gccgggcatg cggcgggcga cgctttgctg cgcgaactgg cgtcgttgat gctgagtatg
     7741 ctgcgttcca gcgacgtgct ggcgcgactc ggtggggatg aatttggtct gctgctacca
     7801 gactgtaatg ttgaaagcgc gcgttttatc gctacacgca ttatcagtgc cgtgaatgac
     7861 tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg gattaccttg
     7921 attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat
     7981 gcctccaaaa atggtggacg gggccgggtg acggtttacg aaccacagca agctgccgca
     8041 catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag
     8101 ttgatgatga tggcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg taatttgtgg
     8161 ctgatttcgc ttaaactctg gagttgcgaa ggcgagatta ttgatgaaca aaaatttcgt
     8221 cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt ccacgaattt
     8281 ttccagctgg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct ccccctttcc
     8341 gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct ggaaaatagc
     8401 cctctaccac cacggttatt acatctgatt attccggcag acgcgatttt agatcacgca
     8461 gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca ggtgggccgc
     8521 gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt
     8581 gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac gattattcag
     8641 gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt acccttagtg
     8701 atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat tgccgatgcc
     8761 caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactgagg gagcgtttgc
     8821 ccattgcctg atgcgacgca tcaggcctac aattcgtccg ttccgtctgg ttgccagcct
     8881 tccgtatacc agatatgcaa cagcgcataa gaacgccagg gcttccagcg ctcggcataa
     8941 cggcggattt gtgccggtgt catccccgga aatcgctgtt taatcagata atcatccggc
     9001 agaaaaacat ctttcgcctg ccagccacgc aaagcgaaat aattcgccgt ccagcgcccg
     9061 atacccggaa aagtttgcag cgttttcatc gcctgctcca catcgcccgg aattgtcatt
     9121 ggtaagctgc cctccagcgc cgcatttgcc agatgaatca gtgcctctgc ccgtttcaac
     9181 ggcatgccta acgcttttaa tgcctgcggg tcggctgctg ccagccgctg aggcgtgggg
     9241 aagcagatat actccggaaa atcatccagc cgttcgccat aaagctgtac aactttggca
     9301 gtcaattttg ccgccatcgc cacgctcacc agttggccta aaatcgcccg cacaccctgc
     9361 tcaaaagcat caatacagcc gggtaaacgc aatccgggcc gcgccgcgcc taacttgccc
     9421 aacgcaccgt taacaatctg cgggttacat tgcagatcaa acaggcggct cattttcgcc
     9481 agacactcag cggcaacggg ttctaaacct gcacttaaat ttatgtgcag agtatggcgg
     9541 gctatatccg gaatagcagt caccacgccg cgatattcgc ccaccgccag actacgggca
     9601 taataactgt ccgcgaccgt ttccacacca ctcactgcac gggcggcgag aaatcccaac
     9661 atccacgacc agtcatacgg cggctgccag ttcagggtat acatcgcatc tccttattca
     9721 tccgctttca gcataaacgt tattcagacg ctacgctttg ctttcatatt ccggttgtcg
     9781 cgacagcaac atttcgctaa agtcacgccc cttcttcacc ggcatgggga ttaattcgtg
     9841 tttattggtt ttgattacgg tacagcaaac tgttcagtgg cggtcatgcg tgacggtaaa
     9901 ccgcaattac tgaaaatgga aaacgacagc acgctgctgc cttcaatgct ttgcgcgcca
     9961 acgcgtgaat cggtaagcga atggctgtac cgccatcatg atgttccggc agacgacgat
    10021 gaaacgcagg cgctgttacg tcgggcgatt cgttataacc gcgaagaaga tatcgatgtt
    10081 acggcgaaaa gcgtgcagtt cggtctttcc tcactggcac agtacattga tgatccagaa
    10141 gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg ccagcggctt aaaaccgcag
    10201 caggtagcgc tgtttgagga tctggtctgc gcaatgatgt tgcacattcg ccagcaggcg
    10261 caggcgcagc tgccagaagc gattactcag gcggtgattg gtcgcccgat caacttccag
    10321 gggctgggcg gcgatgaagc aaacgcccag gcgcaaggga ttctggaacg cgcggcgaag
    10381 cgtgccggat tcagggacgt ggtattccag tacgagccgg tcgcggctgg gctggattac
    10441 gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg atatcggcgg tggtacgact
    10501 gactgttcat tgctgctgat ggggccgcag tggcgttcgc gtctcgatcg tgaagccagc
    10561 ctgctgggtc acagtggttg ccgtattggc ggtaacgatc tggacatcgc actggcgttt
    10621 aaaaacctga tgccgttact gggcatgggt ggcgaaaccg aaaaaggcat cgccctgccg
    10681 attctgccgt ggtggaatgc ggttgccatc aacgacgtac ctgcgcagag tgatttctac
    10741 agtagtgcca acggtcgtct gcttaacgat ctggtacgcg atgcccgcga accggaaaaa
    10801 gtggccctgc tacagaaagt ctggcgtcag cgtttaagct atcgcctggt gcgtagcgca
    10861 gaagagtgca aaattgctct ttcaagcgta gcggaaaccc gcgcctcact gccgtttatc
    10921 agcgatgaac tggctacgct gattagccag caagggctgg aaagcgccct cagccagcca
    10981 ctggcgcgga ttctggaaca ggtgcaactg gccctggata acgcccagga aaaaccggac
    11041 gttatctacc tgaccggcgg tagcgcccgt tcgccgctga ttaaaaaagc gctggcagaa
    11101 cagttgccgg gaattccgat tgcaggcggc gatgactttg gctccgtcac cgccgggctg
    11161 gcacgctggg cggaagtggt gtttcgttaa aagaaaatgg ctcagcagcg gcttaaatct
    11221 gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg gttccatcaa cggcatggtc
    11281 cgtgctggag catgaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgtcgat
    11341 tccagccgat gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt
    11401 atcgcttcat tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgataacc
    11461 gcctgggtca gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct
    11521 gcggctgttt caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga
    11581 atagaggcaa catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca
    11641 gtgtcatgaa gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc
    11701 ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg
    11761 atctggcgga ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc
    11821 tgaacttctg ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgccagc
    11881 acaggaacca aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat
    11941 gctgacagtg cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc
    12001 ggcctgccag tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact
    12061 gacgccatca ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc
    12121 tcttcgccga gatcaataaa atagataacg ccttgattat ccagcgcaca ccacgggcag
    12181 tcggtcaaat gagcgggata aacatgcatt gccgaaacgg gacatttctt taactgttgg
    12241 cgcagagaat caagtgccgc tacccacgct ttagccgtcg gacgcccggt tgccacgcca
    12301 ctttccgtga atgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga
    12361 atcgatcgtg gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg
    12421 aaatgggtaa tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa
    12481 taaggatgcc gaccaccaaa caagacgtga aatatcagca aggcaaggcc aaaattatcg
    12541 tgattcgcgg tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta
    12601 aaatgcgaca caccgacttc gcataaatgc aacgtgccat tagcgttaat ctgaaaggag
    12661 tcgctatcaa tcaataccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg
    12721 tcacccacga cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca
    12781 acatagagta gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggtta
    12841 tagatcatat gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt
    12901 ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg
    12961 tcagctgtcg cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga
    13021 taaatcttgg cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct
    13081 ttgcccagtt cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc
    13141 atggtaacca cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg
    13201 ggctggacaa aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg
    13261 acgccagtcc attgaaaaac ggggtaaaga aaggtacatg aggagaatta tccagcatat
    13321 ttaacgccag tcgctggata ccatccgtaa atgctgcaac tttatgcaca cgctcagtgc
    13381 tggtgaaagt ttccagtctg gaaacggcat cttcatcagt aatgaagtgc gtcatattgg
    13441 catattcacc caccatcggc gtaagcggca attgcagacc gtgaccaaaa tccaccacca
    13501 cgccgccatc gccaatctgc ataatcaagg tgccattagc cgatgaaatc aatcccagaa
    13561 acgtgcaggc aaaatcgcga actgctaatt ctttggcctc tgcctcggca aaaagtcgct
    13621 gacgaatagt cagcacaata tccgtggcga ggatatcatt aagccccaat tccccaccct
    13681 gcactttttg cgacatataa gccatcgctt cattgacggc gagcatcgcg ccttcgccac
    13741 cctgcgagac gctgcctgca ccatcagcaa gaaacatcac cagtaatggt tgctgatcgt
    13801 ttaaccaggc aacctgcatc tggcaggcat cctgacaagg taaatcagcg ctaatgtgcg
    13861 acgttccaac agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg
    13921 cgcttccagc acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga
    13981 taaccagcta aacagttcac ggaattgtaa tccttgcaga ggcaaaggct gacgaacgct
    14041 gatttgtgcc agcgtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt
    14101 cttatcttct tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc
    14161 gccatcggtg atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc
    14221 acgtttcctc tcttcaacca tatccagggc tttggtaatg gcggccccca ttggcgtatc
    14281 cccctgggca aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac
    14341 atgcaccggg ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcggc
    14401 aagcagttca tcacgaaagg ccaccaatcc ggcgttaagt tcattaatag gtctgccgct
    14461 catcgagcca gaaacatcca gcagcagaat gcaggggcaa cgtggttccg ggttactggc
    14521 aaaatcgctg gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta
    14581 tcgaggtccg gtatcatacc gactgcaata atgaaataag agcaaacgaa gaaactttca
    14641 gaattaggcc cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc
    14701 gattgacact aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc
    14761 cctccccctg gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga
    14821 tgacgtgctt catcataacc ctttccttat taaaagccct cttctccggg agaggctttc
    14881 ccgtttcagc gtcccgctga aatcatcggc ttacctcctt tcgccctgaa tgcagtctat
    14941 cgctaacgcg tgaataaaat agtttcctct gtgattattg cgtaacgact cgcaaagaat
    15001 atttgctggc aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca
    15061 tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg
    15121 gaagcttatg actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt
    15181 taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt
    15241 acctcctttc gccatgcact tagtctatcg ctgacttgca gataaaatag tttccagtgt
    15301 gattattgcg taacgtctcg caaagaattt ttgctggcag gatcgcagac ggcaaagcct
    15361 gcggattgac aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc
    15421 ccccgccctg gtagtcttag tatgctgggt agctaatcac taagagcacc acgatgatga
    15481 gtagcttcat catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt
    15541 ttcagcatcc ggctgaaatc tgcggcttac ctcctttcgc cctgtatgta gtctatcgct
    15601 aacgcgtaga taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg
    15661 ccattcagac gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg
    15721 gctacactag tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc
    15781 agttataaat cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc
    15841 tggttctggc aaggccgcaa tgactcccag agtgcagccc caggggcgac gaaacaagcg
    15901 cagcaatcgc cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag
    15961 gcggcgaccg ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattacc
    16021 gccgctaata ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttacatttc
    16081 caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc
    16141 aaagttgcat tagcacaagc ccagggccaa ctggcaaaag ataaagccac gcttgctaac
    16201 gcccgccgtg atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag
    16261 gagctggatg cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa
    16321 gcaagcgtcg ccagcgcgca gctgcaactc gactggagcc gcatcaccgc accagtcgat
    16381 ggtcgcgttg gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc
    16441 ggaattgtgg tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc
    16501 gatatcgcta ctgtagtgca ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg
    16561 gatcgcacca attcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc
    16621 gatgccacaa ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg
    16681 tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg
    16741 atcccaacag ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc
    16801 gaaaacaagg tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg
    16861 atccgtgcag gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc
    16921 gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc
    16981 accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca
    17041 gcacaggcgg cccgtcgcgc ctttttatta tgcgtcctgt cgccaccacg ctgctgatgg
    17101 tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg
    17161 aagtggacta tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca
    17221 tgacctctgc cgttaccgcg ccgctagaac gccagttcgg gcagatgtct ggcctgaaac
    17281 agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat
    17341 taccgctcga tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt
    17401 tgccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga
    17461 tcatgacgct cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg
    17521 aaacccgcgt cgcgcagaaa atctcgcaga tttccggtgt cggactggtg acgctttccg
    17581 gcggtcagcg tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc
    17641 tgaccagcga aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta
    17701 gcctcgacgg cccttcccgt gcggtcacgc tttccgctaa cgaccagatg caatccgccg
    17761 aagagtatcg ccagctaatc atcgcctacc agaacggcgc accgattcgt ctgggcgatg
    17821 tcgcaaccgt agagcaagga gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac
    17881 aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca
    17941 gcatccggca gatgctgcca cagctcaccg agagtctgcc gaaatcggtg aaggtgacag
    18001 tgctttccga tcgcaccacc aatattcgcg catccgtcga tgatactcag tttgaattga
    18061 tgatggctat cgcgctggta gtcatgatca tctacctgtt tttgcgcaat attccggcga
    18121 ccatcattcc cggtgttgct gtaccgctgt cgttaatcgg cacttttgcg gttatggtgt
    18181 ttctcgattt ttcaatcaat aacctgacac tgatggcgtt aactatcgcc accggattcg
    18241 tggtcgatga cgccatcgta gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa
    18301 aaccgttggc ggcggcgctc aaaggcgcgg gtgaaatcgg ctttaccatt atctcgctga
    18361 ccttctcact gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac
    18421 tgttccgcga atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc
    18481 tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga
    18541 accgcttctc ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcagg
    18601 gacttgcgaa agtgctgaat catccgtggc tgaccttaag cgtggcactc agcacgctgc
    18661 tgcttagcgt gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaacg
    18721 gcattattca gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc
    18781 gacaacgcca ggtcgcggac gtgattttgc aggatccagc ggtgcaaagc ctgacctcat
    18841 ttgttggtgt tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca
    18901 aaccgttgga tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg
    18961 tagataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata
    19021 ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca
    19081 gtacctgggt gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct
    19141 ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca
    19201 gccgtctggg gatcagcatg gcggacgtcg ataacgccct gtacaacgcg tttggtcagc
    19261 gactgatttc cactatttat actcaggcta accagtatcg cgtggtgctg gagcacaata
    19321 ccgaaaatac cccaggcctc gcggcgctgg ataccattcg cctgaccagc agcgacggtg
    19381 gcgtggtgcc gttaagctca attgccaaaa ttgagcagcg ttttgcgccg ctttccatca
    19441 accatctgga tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc
    19501 tgggcgatgc ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata
    19561 tcaccacgca gttccagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct
    19621 ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct
    19681 ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggagtcggc gcactgctgg
    19741 cgttaatgat tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga
    19801 tcggtatcgt gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg
    19861 agcaaggcat gtcgccgcgc gatgccatct accaggcttg tctgttgcgt tttcgtccga
    19921 tcctgatgac cactctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg
    19981 tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggcctg attgtcagcc
    20041 aggtgctgac gctgtttacc acgccggtga tttatttgct gttcgaccgc ctggcattgt
    20101 ggaccaaaag ccgctttgcc cgtcatgaag aggaggcgta agtgaagttt tttgccctct
    20161 tcatttaccg tccggtggcg acgattttac tgtcggttgc cattaccctg tgcggcatac
    20221 tgggcttccg tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg
    20281 tcagcgcctc gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc
    20341 tggagcgctc acttgggcgc attgccggag tcagtgaaat gacctccagc agttcgctcg
    20401 gcagcacgcg tattattttg cagtttgatt ttgaccggga tatcaacggc gcagcgcgtg
    20461 atgtgcaggc ggcgatcaac gctgcacaaa gtttgctgcc cagtgggatg cccagccgcc
    20521 cgacctatcg caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacgtccg
    20581 atacttattc gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt
    20641 cgcaaatcga cggtgttggt gatgtcgatg tcggaggcag ctcactgccc gccgtacgcg
    20701 tcgggctgaa tccgcaggcg ctgtttaatc agggcgtgtc gctggacgac gtacgcaccg
    20761 ccgtcagcaa tgccaacgtg cgtaaaccgc agggcgcgct ggaagatggc actcaccgct
    20821 ggcagatcca gaccaatgat gagctaaaaa ccgccgctga atatcagccg ttgattattc
    20881 actacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc gactcagtgc
    20941 aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattttactg atgatccgca
    21001 aactgccgga agccaatatt atccagacgg ttgacagcat ccgggcaaaa ttaccggagt
    21061 tgcaggaaac cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca
    21121 ttcgcgcctc gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc
    21181 tggtggtgtt tttattcctg cgctcgggtc gcgccactat tattcccgcc gtttcggtgc
    21241 cggtttcgct gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc
    21301 tttcgttaat ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc
    21361 tggaaaacat tgcacgtcat ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag
    21421 gtactcgcga agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc
    21481 tgccgctgct gttgatgggc ggattgccgg gccgactgtt acgcgaattt gccgtgacgc
    21541 tttctgtcgc cattggtata tcgttgctgg tttctctgac attaacgcca atgatgtgtg
    21601 gctggatgct gaaagccagc aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca
    21661 tgttggtagc cctgcaacaa ggctacggca agtcactgaa atgggtgctc aatcataccc
    21721 gtctggtggg cgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga
    21781 tcccgaaaac cttcttcccg gagcaggaca ctggcgtgtt gatgggcggg attcaggcgg
    21841 atcagagtat ttcgtttcag gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc
    21901 gtgacgatcc ggcagtggat aatgtcaccg gctttacagg cggttcgcga gtgaacagcg
    21961 ggatgatgtt tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta
    22021 tcgaccgtct gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg
    22081 tacaggatat tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat
    22141 ccgacgacct ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt
    22201 tgccggaact ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg
    22261 tttacgaccg cgacaccatg gcacggctgg gaatcgacgt acaagccgcc aacagtctgt
    22321 taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata
    22381 aagtggtgat ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt
    22441 tcgttatcaa taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg
    22501 cgaatgcccc actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttta
    22561 acctgccgac cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc
    22621 agcttggtgt gccttcgacg gtgcgcggca gttttgccgg cacggcgcag gtgttccagg
    22681 agacgatgaa ctcgcaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc
    22741 tgggtatcct ttacgagagt tacgtacatc cgctgacgat tctctccacc ctgccctcgg
    22801 cgggcgttgg agcgctgttg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc
    22861 tgatagggat catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt
    22921 ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct
    22981 gtctgctgcg ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggtgcgctgc
    23041 cgctggtatt gtcgggcggc gacggctcgg agctgcggca acccctgggg atcaccattg
    23101 tcggcggact ggtaatgagc cagctcctta cgctgtatac cacgccggtg gtgtatctct
    23161 ttttcgaccg tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa
    23221 tgacagatct tcccgacagc acccgttggc aattgtggat tgtggctttc ggcttcttta
    23281 tgcagtcgct ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg
    23341 gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga
    23401 tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg
    23461 ccatcgtgct gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac
    23521 tgttgctggc acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcagat
    23581 tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt
    23641 tacccggtca ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg
    23701 cgtcgtggca ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca
    23761 cattgatgtt aatgccgaac tataccatgc agacgcggcg cttcgatctc tccggctttt
    23821 tattgctagc ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag
    23881 gtttatcgcc gctgacgatt gatggcctgg tcgcagttgg cgtggtggca ctggtgcttt
    23941 atttgctgca cgccagaaat aacaaccgcg ctcttttcag tctgaaactg ttccgcactc
    24001 gtaccttttc gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc
    24061 cctttatgac accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac
    24121 tgatgatgat cccgatggtg cttggcagca tgggaatgaa gcgaattgtt gtacaggtgg
    24181 tgaatcgctt tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca
    24241 ccctgttgtt tatgactacc gctctgctgg gctggtacta cgttttgccg ttcgtcctgt
    24301 ttttacaagg gatggtcaac tcgacgcgtt tctcctccat gaacaccctg acgctgaaag
    24361 atctcccgga caatctggcg agcagcggaa acagcctgct gtcgatgatt atgcaattgt
    24421 cgatgagtat cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg
    24481 tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt
    24541 ttatcatcgc ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg
    24601 tagctatttc gcggcgaaaa aggagcgcgc aatgaagttc tggcgacctg gtattaccgg
    24661 caaactgttt ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc
    24721 ggtgcgtatc agttttgagc gtggctttat tgattacatc aagcatggta atgaacagcg
    24781 attacaactg ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt
    24841 cctgcgcaac aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc
    24901 ggaagataaa cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt
    24961 tgatcaaaac aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg
    25021 gcgacccatt ctggtcaacg gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg
    25081 gttaacgcgc aatactgata tcaatttcga taaacaacag cggcaaacca gctggttgat
    25141 tgtcgccctg gcaacgttac tcgcggcact tgccactttt ctgctggcgc gcggtttact
    25201 ggcaccggta aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac
    25261 ccgcgtaacg cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct
    25321 tgccagcaca ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttctca
    25381 cgaactgcgt acgccattag cggtgctgcg cggtgaactg gaagccattc aggatggcgt
    25441 gcgtaaattc acgccggaga cggtggcgtc tttacaggcg gaggtcggta cactgaccaa
    25501 actggttgac gatctccatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa
    25561 agcaccggta gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt
    25621 cgccagtcgt ggcctgaaac tgcaattttc cctgccagac agtattaccg tatttggcga
    25681 tcgcgaccgt ttaatgcagt tattcaataa cttactggaa aacagcctgc gctacactga
    25741 cagcggcggc agcctgcaaa tctctgccgg gcagcgcgac aaaacggtgc gcctgacctt
    25801 tgccgacagt gcgccaggtg tcagtgacga tcagctacaa aaattgtttg aacgttttta
    25861 tcgcaccgaa ggttcccgca accgtgccag cggcggttcc gggctggggc tggcgatttg
    25921 cctgaacatt gttgaagcac ataatggtcg cattattgct gcccattcgc cttttggcgg
    25981 ggtaagcatt acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag
    26041 ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg
    26101 cagttgctca ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc
    26161 gatcaggtac tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt ggatctgatg
    26221 ctccctggca tcgatggctt gacgctgtgc cgggaaattc gtcgtttttc tgacattcct
    26281 atcgtgatgg tgacggcaaa aatcgaagag atcgaccgcc tgctcggact ggagattggc
    26341 gcagatgact acatctgcaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc
    26401 attttgcgcc gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagcccgttg
    26461 attatcgacg aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacacct
    26521 gcggaatttc gtctgctgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag
    26581 catttgctca atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgacagc
    26641 cacattaaaa acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc
    26701 gccgtttacg gcgttggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag
    26761 cgacatgact ttgttagccg gagatggcgt tcagaccaga tccggcaaca ttatcccacg
    26821 catggtcagc aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac
    26881 tttaatcggt aaacagcacc tttagatgct gttttcgata cgcaatttca atcaaggagt
    26941 cattatggct ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct
    27001 aaaagcgggc aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc
    27061 ggaaaagggc atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa
    27121 aacggcaagt aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg
    27181 ctccagccag atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc
    27241 caatggcaca agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt
    27301 tggcgtccgg ctttatatcc aaataacgct tagttattaa taattaagag taaccgccgc
    27361 cattaaaata gtgggcactg acgagcactc gttgtgcttt ctttgtttac catcccatac
    27421 ttatcctcct gaacatgttc atgtgccaaa atatcttcta atactttcgt gacgctatcc
    27481 ggtttatgac atacactgat gtaagatttt tacttacagg cgggcgtaaa atggccagaa
    27541 cgatgacggt tgatctcggc gatgagttgc gtgaatttat cgagtcctta attgaatccg
    27601 gcgactatcg cacccaaagc gaagtgatcc gtgagtcact gcgattactt cgagaaaagc
    27661 aagcagagtc tcgcctgcaa gcgttacgtg acatgctcgc agaagggttg agtagtggtg
    27721 aagctcagcc gtgggaaaag gacgcgttct tacggaaggt gaaagcaggg ataaggaaat
    27781 aatgtgcgta ttattaaact catgccaaaa gccaatgagg atttagaagg catctggtat
    27841 tacagctatc accatttcgg cgaaccacaa gccgacagat atgtggaaca tctttcagac
    27901 gttctccaga ttctgagtaa caataatata ggtacgccca gacctgaatt aggcgaaggt
    27961 atttttgttc ttccttttga acgccacgtc atttatttcc tgcaatcacc aggggaaatt
    28021 atcgtcattc gtatccttaa ccaaaaccag gacgccacca gacatttaca ctggagctaa
    28081 acccgccgat tgcgctacaa tgcccgccct taaagtgggg gcactcccct aaccgcttca
    28141 tcaggtgaag cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc
    28201 ctttccccgg cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct
    28261 gtttatgcgg gccagccgcg ttattccctg cgtgtgcgca acaacgaatt caaccacgaa
    28321 aatcttcagc tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc
    28381 aacattgcac cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg
    28441 gaaatggggc cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag
    28501 cacttccctg aaatgccgat ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg
    28561 gtgaaattct ggcagcaaat gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg
    28621 gaagagattg aagagatccg caatcaggtg ccggatatgg agatcgagat cttcgttcac
    28681 ggcgcgctgt gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc
    28741 gacccgaacc agggcacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg
    28801 aaagaagatg atgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt
    28861 gagccgacgc tgggtatcgg cgcaccaacc gacaaagtgt ttatgatcga agaggcccag
    28921 cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg
    28981 aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg
    29041 aaaatcgaag gtcgtaccaa atctttctac tattgtgcac gcaccgcaca ggtttaccgc
    29101 aaagctatcg atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg
    29161 gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat
    29221 tatcagaact acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt
    29281 accggtgagc gcaaggggga cctcgcggcg gtagcggtga aaaataaatt ctccgttggc
    29341 gacagccttg agctgatgac gccgcaaggc aacattaatt ttacccttga gcacatggaa
    29401 aacgccaaag gcgaagctat gccgatagca ccaggcgatg gttatactgt gtggctcccg
    29461 gtcccgcagg atcttgagct caattacgcg ctgctgatgc gtaatttctc cggggaaacc
    29521 acgcgtaatc cccacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt
    29581 agaaaccgat cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat
    29641 aacccataaa atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac
    29701 acgggcttat tttttatgca taagccctat ccctggtcac cgtcttccat tgaccacatc
    29761 gatagaatct cccttcatag cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt
    29821 tccggctaag cacaaccaga acgcgcatgt ttgatgctta ccaaaaaata ttctcactct
    29881 ccacatttga atgtcagacg agcgacgcca tgtaatcctg caccttctgt cttcaggtca
    29941 actatctgca tttttttgcc ctgagtaaca cagaaatggg ctgcatcatt ttttactata
    30001 ttttctgcac cagatattct gcccctggct aaagaagctt cggcttcggt gtagtattga
    30061 ttatcgagtt tacgctgaat attactttta tatgcaagac caaatttacc gatacttgtc
    30121 tcatcattat gcacagcaca accagacata ataaaaatac taattaatga tatagcagct
    30181 atc
//
LOCUS       DAPHDX010000048        30120 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPHDX010000048 DAPHDX010000000
VERSION     DAPHDX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.048"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="RH058_002083"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="RH058_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV3137673.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="RH058_002084"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="RH058_002084"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV3137674.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="RH058_002085"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="RH058_002085"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV3137675.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="RH058_002086"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="RH058_002086"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV3137676.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="RH058_002087"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="RH058_002087"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV3137677.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPAEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="RH058_002088"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="RH058_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV3137678.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /locus_tag="RH058_002089"
     CDS             complement(8992..9609)
                     /locus_tag="RH058_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV3137679.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="RH058_002090"
     CDS             complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="RH058_002090"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV3137680.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVALAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="RH058_002091"
     CDS             complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="RH058_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV3137681.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVHELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEYDWNAAFGTT
                     HL"
     gene            complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="RH058_002092"
     CDS             complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="RH058_002092"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3137682.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFPM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            14482..15561
                     /locus_tag="RH058_002093"
     CDS             14482..15561
                     /locus_tag="RH058_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV3137683.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15576..15896
                     /locus_tag="RH058_002094"
     CDS             15576..15896
                     /locus_tag="RH058_002094"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV3137684.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            15947..18244
                     /locus_tag="RH058_002095"
     CDS             15947..18244
                     /locus_tag="RH058_002095"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV3137685.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDDVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            18210..19088
                     /locus_tag="RH058_002096"
     CDS             18210..19088
                     /locus_tag="RH058_002096"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV3137686.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            19090..19431
                     /locus_tag="RH058_002097"
     CDS             19090..19431
                     /locus_tag="RH058_002097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV3137687.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19418..20269)
                     /locus_tag="RH058_002098"
     CDS             complement(19418..20269)
                     /locus_tag="RH058_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3137688.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(20484..22217)
                     /locus_tag="RH058_002099"
     CDS             complement(20484..22217)
                     /locus_tag="RH058_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV3137689.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(22400..25051)
                     /gene="ppc"
                     /locus_tag="RH058_002100"
     CDS             complement(22400..25051)
                     /gene="ppc"
                     /locus_tag="RH058_002100"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV3137690.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(25403..26554)
                     /gene="argE"
                     /locus_tag="RH058_002101"
     CDS             complement(25403..26554)
                     /gene="argE"
                     /locus_tag="RH058_002101"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV3137691.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            26708..27712
                     /gene="argC"
                     /locus_tag="RH058_002102"
     CDS             26708..27712
                     /gene="argC"
                     /locus_tag="RH058_002102"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV3137692.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            27720..28496
                     /gene="argB"
                     /locus_tag="RH058_002103"
     CDS             27720..28496
                     /gene="argB"
                     /locus_tag="RH058_002103"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV3137693.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            28557..29930
                     /gene="argH"
                     /locus_tag="RH058_002104"
     CDS             28557..29930
                     /gene="argH"
                     /locus_tag="RH058_002104"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV3137694.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6774 a         7687 c         8429 g         7230 t
ORIGIN      
        1 gttccactcc gcgccgctct tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca cagttaatgt gggcactggc cgtaaacccg
      421 catagtttac cgtacaggcg ctaccgttac atcgggtaat ccacctgctg gcgtgataat
      481 gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaccacaat gtttactctg gtgcctgaca tttcaccaat
      601 aaagcccagg gaacatcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccc atccatcttt ccagtaccta
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgtg gcaacccaac
      781 gcgcgatgtg gttcagcgag cactggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aacgaccgtc tttttgaaac ctggcgatct
      901 gctggtagcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgtggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 gctggcagaa aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttaggtgccg atctggtgtt
     1201 gcattcatgc acgaaatatc tgaacggtca ctcagacgtg gtggccggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgcatgga
     1381 gttggcccag cgtaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgtcagca
     1501 aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgagc gggctgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatctcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacctg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtggggcacg gtgaagtgtg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gccagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct ggtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtta tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgccac cgtcggtgcg
     3601 ggcttgccga tcaaccacac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagcggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
     3781 gacgacctct ccggcaaaga cgtaatgcgc aagctggtaa ttctggcgcg tgaagcgggt
     3841 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaat
     4021 ggcaaagcgc gtgtgggcgt ggaagcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccgtgcgata acgtctttgc catcgaaagc cgctggtatc gcgacaaccc gctggtgatc
     4141 cgtgggcctg gcgcggggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtc
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatga agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aatggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ttgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagcca gaaatgtatg cttctgacct ggtgacgcta
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaagt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgcttaat ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt cttcgatgtc gaaagctacc tgcgttttcg tgaccgctgt
     5161 gtatcggcgg gcattgatgt ggaaattatt ccgggaattt tgccggtatc taactttaaa
     5221 caggcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcgcaaatg
     5281 ttcgacggcc tggatgatga tgccgaaacc cgcaaactgg tgggcgcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acactgtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgtaaa aataaggttg tcagctttgt tttcgccctc cttccttgaa
     5581 gaataggaat ctaaaaccct tttttataaa gcagttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatatccata acacgacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggccagccga
     6061 ctggggcagc tacgccggtc tgtttattcg tatggcctgg cacggcgcag gtacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcaggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaacgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggctgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 tctgttcaag tatgagtggg tacagacccg cagtccggct ggagcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac
     6901 aatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg gccgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggc cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct
     7621 ggtgggcggg atgcgcgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg accgacgaat cgaaagagct gtttgaaggc cgtgaccgcg aaaccggcga
     7801 agtgaaatac actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtggctgaa gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgcct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtct ccacaggaaa
     8041 actacacact ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcaa tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgcct
     8581 atacacccgt catccgcgcg tggatttatt atcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccga tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt ggagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtgggagtt
     9721 tcagattact ggcacagagt gccggatgcg gagtgaacgc cttatccggc ctacaaaagc
     9781 acgcatattc aatatattgc agagatttcg taggcttgat aaacgtagcg catcaggcaa
     9841 ttttgcgttc aaactcccgg acaagccggg agtttggagt aggttattcc cactcttgca
     9901 ggaaacgctg accgtactgg tcggctacca gcagagcggc gtaaacctga tctggcgtcg
     9961 cgccgccagg catgttgtga atggtttcac cttctgcaca tgccgcttct gccacaattc
    10021 gcattttcgc cgggacatct tctttaatat ccagttgagc gagagttatt ggcaaaccta
    10081 ccgcatggct aagtgcagct acggtttcga tttcctcaac cggtgcgttt tccagaacca
    10141 gctgcgtcag cgtaccaaat gccacttttt caccgtgata atagtgatgc gcgtccggga
    10201 tagcggtcag gccgttatgc accgcgtgag ccgccgccag cccgccgctt tcaaaaccaa
    10261 cgccgctcaa ataggtgttc gcttcaatca cgcgctccag cgccggggtt actacatgct
    10321 gttcggcggc aagcatcgct ttttcgcctt cttccagcag ggtgttgtag cacagttcag
    10381 ccagtgccag cgccgcttgg gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag
    10441 agcaagcacg cgcttcaaac caggttgcca gcgcatcgcc gatacccgct gctaacagac
    10501 gtgcaggtgc gcccgcgacg attttggtgt cgacaatgac catattcggg ttatttggca
    10561 acagcagata gcggtcaaac tcaccctcat cggtgtagat aacagacaat gcgctgcacg
    10621 gtgcatcggt agaggcgata gtcggtgcga gcgctaccgg aacacccatg aaatgtgcca
    10681 gtgctttggc agtatcgagg gtttttccgc caccgatacc gagaattgcg ccacactgcg
    10741 cagtctccgc gatgccacgc agacggtcga tctcattttg cgaacattca ccgccaaacg
    10801 gcgcaatttc tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagcaa
    10861 aacctaaaac aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt
    10921 cgcccagacg attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgc
    10981 ggtccataat tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtattca
    11041 aacttctcta tagctgactc taccgcaggc gtattgagca tttgttgcgc tacatctaaa
    11101 ggcagggtga tggactcaca tcctgccagc aaacaatcca gtgcctgacg cggcgtttta
    11161 aagctggctg caagcaccat gctttctggc gcgtgcattt ctaacagcgc ttgcagctca
    11221 tgaaccgtac gaataccgtc tccgccctgg gcgtctacgc ggttaacata cggagcaacg
    11281 tattttgctc ctgccagtgc ggctaataac ccttgtgcgg cgctatatac agcggtgcca
    11341 agtgtagtaa taccctcttt tttcagtatt ttaattgctg ccagaccttc ggaagtcacc
    11401 gggattttca ccacaatgcc cggaatggcg tcgcgcagac gcttcgcttc ttccaccatc
    11461 ccctgcgcgt cgcggctcat ggtctgagca aacagaatgc cctcatcacc aatcgctttt
    11521 tgcagacgcg gcagcacttc ccagatggac tccttgctgg cagcgataat gctcgggtta
    11581 gttgtcaccc cggcaatcgg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg
    11641 gtgtccagat acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc
    11701 tggcgatagc gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga
    11761 tgcggcgagc gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca
    11821 gggttaacat aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gcttgcagac
    11881 gcgccacgct gatggtggat tgctcaatgt gttctgattt gctgtgtgga atggcaaaac
    11941 taaagcccag accggtagag aaaacggcct cgcgcgccca taagtcggct tccagtttac
    12001 gcggatagcg gcagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt
    12061 ctttgctgcg ccagtcactt tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct
    12121 gttgggtcat ggggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca
    12181 ttgcctggtt gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg
    12241 ccgccggaat tgatggtgcg ctcatgctga gttcatccag ccctaagccg accagcaacg
    12301 gcagcacgga acctttcgct cccagctcac cgcacagacc aatccatttg ccctggcgat
    12361 gcaccgcctg cacggcgtaa tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt
    12421 gacgagtaac cttagcgtta tcgcgatcca ctgccagcag atattgcgtc aggtcattac
    12481 taccaatact aaagaaatca atctcttcgc agcattgatc gatgatgaac atcaccgacg
    12541 gtacttccag cataatgcca agctggatct tctcatcaaa cggaatgtgt tcgttacgca
    12601 gttgctgttt ggcttccgcc agtttttctt tcacccataa gatctcttcc attgaggaga
    12661 tcatcgggat catgattttc aggctgccgt gagcggaggc gcgcaggatc gaccgtagtt
    12721 gtgtggtgaa cagcgaggcg tattcttcat aaatacgcac agcgcgataa ccgaggaacg
    12781 ggttagcttc tgccggaatg ttcagataat caacgggttt gtcgccgcca atgtccatag
    12841 tgcgcacaat aatgctgcgt ccgttggcag actccagcgc ctgacaaaaa atgttgtaca
    12901 gctcgctttc gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaacaaac
    12961 caacgccttc cgcaccattg ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag
    13021 cggcaatttc aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct
    13081 gttgctcacg cagggcgtcc tgtacgcggg cttcttgctg ataataacga gctacggctt
    13141 ctcctggctc aaccacaatc gccccagcgt tgccgtcgat ataaatcgtt tgatgctgcc
    13201 acggagtaag ggcatcaata tctacaccaa ccagtgtcgg aatgttgaac gaacgggcaa
    13261 gaattacggt atgtgaggtg gtgccgccac ttttcagtaa cagacctttg aggtgatttt
    13321 tatccagttc gaggaactgg ctgggagtca gttcatcagc catacaaatg gcgggctgcg
    13381 tcagtttgcc cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc
    13441 agacgtcgcg tacgtccagg gcacgttctt gcaggtagct gctgctggaa cgggcgaact
    13501 cttcgcaaaa gtgattcgca ctggtgacaa tcgcttcggc gcagcttaat ccggcgctga
    13561 cgcctgccag taaatgttcg cgcagggaag tatcgccagc cagggatcgg tgcgcttcca
    13621 gaatcgcgct ggtggcaccg tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca
    13681 gcgtcaggcc gttttccagt gcggattgct cggcgtcaac gcctttggct gcgggaagat
    13741 tacccagcgc attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccgc
    13801 tgcacacggt gcgggcgcgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg
    13861 gttccagttc gtcagattta acttccgcca gcggcgcatc gcagtggggg aattcatcgc
    13921 gcagccattg gcttaaacgc tggtgcgctt cctgttcgtc ggccccggaa atcagtaact
    13981 ggcagttatc gcccgccagc gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc
    14041 ccttgcggtc agtgcgcagg ttatgccact caatttgtga tgaaaaagta ttacacagcg
    14101 tttcaacgtg gcttgccgga cgtgcatgta cgccgttagg tagctcacaa ataaattcca
    14161 caatcagggc cattgcctct ctcccggtac ggttttctgt ttacaggata agagcgcacg
    14221 gcaacggcct gccatgtgac aaatctgcca aaagctggac aaatgtaatg taaccgtcaa
    14281 tttgcgacgc gtctcacaag acgctgtttt gcggcatgct tccggtttat cgcaagttat
    14341 gaggcggatc gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca
    14401 ctgtttgctc cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt
    14461 tcgcaagccc gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag
    14521 catttaatga cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg
    14581 gcggtttccg tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat
    14641 ctgaaaaaac tgtttgatat cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgca
    14701 gcgtacatcg gttattccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc
    14761 tgggttggta acagctttgg tgcgggcttc tttggtgcac tgatcgccgg gattatcggc
    14821 ggcatcgtgg tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg
    14881 cctatcttca ttattcctat cgtcggcaca ctgattaccg caggcatcat gatgtggggc
    14941 ttgggcgagc ctgtaggggc gttgaccaac agcctgactc agtggcttca ggggatgcag
    15001 cagggcagca ttgttatgct ggcggtgatc atgggtctga tgctggcgtt cgatatgggc
    15061 ggtccggtta acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat
    15121 accgtggtgg ccatcgctgc cgtgggtatt tgtatcccgc cgctggggat ggggctggcg
    15181 acgctgattg gtcgtaaaaa tttctccgca gaagagcgcg aaaccggcaa agcggcactg
    15241 gtgatgggct gtgttggcgt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg
    15301 cgtgtcattc cttccatcat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc
    15361 ggtgcgcagt gctatgcagg ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag
    15421 ctgggttata tcgcagcagt ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg
    15481 ctgaaaagtc tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat
    15541 ttggattttg aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg
    15601 catgtccttc aggtgttgcc catacctata tggcagcaga ggcgctggaa agtgccgcga
    15661 aagcgaaagg ctgggaagtg aaagtagaaa cccaggggtc aattggtctg gaaaatgaac
    15721 tgactgcgga agatgtggcg agcgctgata tggtgattct gaccaaagat atcggcatca
    15781 agtttgaaga gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa
    15841 aacgtgctga cgccattatg agcaaaattg aagctcacct ggcgcaaacc gcttaagttc
    15901 ttctcccgct cgcaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat
    15961 ctctcgcctc aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct
    16021 gctttatacc gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa
    16081 ggcaacagcg tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc
    16141 cggtaaccgc accgtaaaac cgcgcgccgg gattatgtcg ccggaaatgg acccttactg
    16201 gctgctgaaa gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagcga
    16261 agaagataaa cgtatctacc gcgaagagtt gttcccgtac tgggaaaaac gttcgatgaa
    16321 agatttcatc aacgggcaga tgacagatga agtaaaagcc gcgaccagca cgcagatttt
    16381 cagcatcaac cagacagata aaggccaggg gcacattatt attgattacc cacgcctgtt
    16441 gaatcacggg ctgggggagc tggtggcaca gatgcagcaa cattgtcagc aacagccgga
    16501 gaatcacttt tatcaggcag cgctgttact gctggaagcc tcgcagaaac acattttgcg
    16561 ttacgccgaa ctggcggaaa cgatggcggc aaactgcaca gatgcccagc gtcgcgaaga
    16621 gctgctgact attgcggaga tctcccgcca taacgcccaa cataagccgc agacgttctg
    16681 gcaggcgtgc cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag
    16741 ttcgctatcg ttggggcgct tcgaccagta tatgttgccg ttctatcaga catcattaac
    16801 ccagggcgaa gatgcggcgt tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa
    16861 cgacatcgtg ctgttgcgct ccaccagtag cgcgcgttat ttcgccggtt tcccgaccgg
    16921 ctataccgca ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc
    16981 gttcctttgc cttgacgcct atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg
    17041 cactaacgcc ttgatcgaca cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg
    17101 caccggtatt ccgcaaatct ttaacgatga cgtggtggtg ccagcgttcc tcaaccgtgg
    17161 cgtttcgctg gaagatgcgc gcgactattc cgtagtgggc tgtgtggaat tatctattcc
    17221 cggcagaacc tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat
    17281 ctgcctgtat gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat
    17341 ccgcgccaag atcagccact acatcaccct gatggttgag ggcagcaata tttgtgatat
    17401 cggccatcgc gactgggcac ctgtaccgct gctctcgtct tttatcagcg attgtctgga
    17461 aaaaggccgc gatattaccg atggcggcgc gcgttataac ttctccggcg tacaggggat
    17521 cggtatcgcc aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca
    17581 gcgtttaagt tttgacgaat tgctgtcggt attaaaagcc aactttgcaa cgccagaagg
    17641 cgaaaaagtc cgtgctcgct taattaaccg cttcgagaaa tacggtaacg atatcgacga
    17701 ggtggataac atcagcgccg aactgttacg ccactactgc aaagaagtgg aaaaatacca
    17761 gaacccgcgc ggcggctact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    17821 gggatcggtg gttggagcga cgccagacgg tcgttttgcc ggagaacagc tggcagacgg
    17881 cggcttgtca cccatgctgg gccaggacgc acaagggcca acagcggtac tgaagtcagt
    17941 cagtaagctc gataacacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    18001 agcgaccctg gaaggtgaag caggattacg caaactggcc gacttcttac gggcatttac
    18061 ccagcttaag ttacaacata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    18121 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    18181 ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    18241 gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt cagcgttatt
    18301 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    18361 gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat ccagacagta cgcagagagg
    18421 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggtgcgt
    18481 ttgaacggat tggtcgcgat atcagccttg acgcgctgga acgggaagtg atgaaagatg
    18541 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    18601 aggcggagtt tgctacccgt tttttacagc ggctgcggtt gtggggcgtg tcatgcgcca
    18661 ttgaaactgc cggagacgcg ccagccagca agctattacc actggcgaaa ttgtgcgatg
    18721 aagtgctgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    18781 acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc aacgtgatcc
    18841 cgcgtttacc gttgatccct ggtttcacgc tcagccggga gaatatgcag caggcgctgg
    18901 atgtactgat cccgctgaaa atcaggcaga tccatctgtt accgtttcat cagtacggcg
    18961 aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt
    19021 cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    19081 gaggttaaaa tggcatacct ggtggcagta accgcctgcg taagcggcgt ggcgcatact
    19141 tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt
    19201 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgtcgggcg
    19261 gatgttgctc tgttgattac ggatatcgag cttgccggtg ccgagcgatt tgaacattgc
    19321 cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt aatgagcgcg
    19381 gtgcgcaaag tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt
    19441 ctgtcagttg gctgtggtac tgccggcgat attccgacgg cgagcgttcg gtgtttttac
    19501 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    19561 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    19621 attcgttaaa accaatggcc cccgtttttt gaaacaggtg cgagagatag tttggtgaaa
    19681 tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    19741 aatcgcgaat agcttcaaat agtgcctggc tgcgtgaggc ggtctggatt tggctgccga
    19801 gcaaatcgcg gcagtggcta agcaggctgg cgacgataag ccttgccgtt tgctgctcct
    19861 gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctatg cgtgggccgc
    19921 gccgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc
    19981 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt agtagcgggg gcttgccatt
    20041 gtggaaaatt ccagccacca gcgggaacgt acagcacatc gccgggaact aacgttgctc
    20101 cagcgccggt atcaacaaac tcaccttcca gcactatttc cagccgtgga aaatcgacct
    20161 gatacgcaag atcgggcgcc ggaccgttac tgctggcaaa gtaaatctgg cgcagggaca
    20221 gcgggccgtt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    20281 aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg
    20341 gcctgagggg agttcgatgt aaacattgcg gcagttgtat tgccggatgt ggcgtaaacg
    20401 ccttattcag tctaccgccg cgatctgtag gccggataag acgcgtcaag cgtcgcatcc
    20461 ggcaaatagc gcctggctga taattactga ttacctacct ggtcgccata cggcagagtg
    20521 tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa tccagcgggt agtttctttg
    20581 aactgcggat tcaccactga acgggttgga tcgtaaccgt cgtaagataa gcccgccaaa
    20641 tctgaccagg tgtggatcag ttccgccagg ctgtatttac gatcgacatc ctgtgagaaa
    20701 tcacggggat gagtcgcttg ccatttttcc gacgtccaca gcaggaacgg aatggtgtac
    20761 atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt tgtgcggcgg cgtgtcgtaa
    20821 acctcttcac cgtggtcaga gaagtaaacc aggaatccgt tcgggtctgc cgctttaaag
    20881 tctttaatca ggctggcaac cacatgatcg ttatacaggt tagcgttgtc ataatcgtta
    20941 tatgattcca gctcttctgc gcttaatcct ggcggaacat gatcggtatt gccatcaaac
    21001 ttgccctgat tttccgggta gcggtatttg tatttgatat gcgtacccag cagatgaacg
    21061 atgatcagtt tcttcggcgc agggtcattc agcacatcct ggaacggctt cagcacgttg
    21121 gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca tgtagtactg cttgtcggtc
    21181 tggcgcgaaa ataccgtcag catggtattg cgggcggtca tcgtctgctg gttggtgatc
    21241 cagaaggttt tataacccgc ctgtttcatc atgttcatca gcgacggctg cgtcagatac
    21301 agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt gcaggatttc aatggtgtac
    21361 ggacgagacg taactacgtt attaaacacg gtcagattcg gatcggtttt atgcagcgca
    21421 tccagctccg gcgtggtttc acgcggataa ccgtacagac tcatgcgtcc gcgctgggtc
    21481 gactcgccaa tcaccagcac taaagtgcgc ggttcgttac ccgattcatc tttgaaatta
    21541 gccagtggcg gcaaggcatt attttcattc agtaactttg ttagcgagtt tagttgctga
    21601 cgatactgat aatagccggt caggaattgc cacggtgcgg caggctccat acgcgaggcc
    21661 aggttatcca acgttttctc aaacggcttg tttttgataa acgtattcat ggcgatcgga
    21721 tgcagaatca agccataaag cagggcaaaa gagacaacat aacgccacgg ctttggaata
    21781 tagaccgggc gcaggcgtgt ccacagcaga actgccaccg ccgtataggc cagcgcgata
    21841 agcacaattt tcaggctgaa atactggctt aaatactcgc tggcttcgtt ggtgttggtt
    21901 tcgaacatca caaacagaac gctctgcgag aactcctgcc cgtagatgac gtagtagcat
    21961 agcgccgcca gagaggccgc ccatagcacc acgccgatta ctgcggcaat aattttaatc
    22021 cgcttcggaa agaggaatac cgggatcaac cacagcgagc tgaataacag cgagtcgcga
    22081 atgccgttgg tgccactata accactgatg taaataatgg cctgtagcag agtagagaaa
    22141 aaccaaaagt agagcagtgc ccaacccagg gctttccagc taaaaagggg tttagcctgg
    22201 acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa atatcgccga atgttacgac
    22261 aattccttaa ggatatctga aggtatattc agaatttgaa taaaatgcag acagaaatat
    22321 attgaaaacg agggtgttag aacagaagta tttcagaaaa ccctcgcgca aaagcacgag
    22381 ggtttgcaga agaggaagat tagccggtat tacgcatacc tgccgcaatc ccggcaatag
    22441 tgaccattaa cgcttgttcg acgcgaggat ccggttcctg gccttctttt tctgcctggc
    22501 gggagcggtg cagtaactcg gcctgcaata cgttcagcgg gtcggtgtaa atattccgta
    22561 gctgaataga ctctgcaatc cacggcagat cggccatcag atgggaatcg ttggcaatcg
    22621 ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa ctctttacct aacggccaca
    22681 gtgctttgtc taccaggcgt tggtcatagt attccgccag ccacaggtct gctttggcga
    22741 agaccatctc cagcatgccg agacgcgtcg agaagaatgg ccaatcgcgg cacatggctt
    22801 ccagctcgct ctgtttgcca tcttcgacca ctttttgcag cgccgtacct gcacccagcc
    22861 aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat ccacggtatg gcgcgcagcg
    22921 actcgacgcc gccggttggg cgacgtttcg ccggacgtga acccaacggc agtttgccca
    22981 attcttgttc cggcgtagcg gagcggaagt aaggcacaaa atctttgttt tcacgtacgt
    23041 agccacggta gagatcgcag gagatgactg acagttcatc cataatgcga cgccagctct
    23101 ctttcggctc aggtggtggc agcaggttgg cttccagaat cgccccggtg tacagcgaca
    23161 ggctgctgac ggtgatttct ggcagaccat atttaaagcg gatcatctcg ccctgttcag
    23221 tcacgcgcag gccacctttc aggcttcctg gcggttgtga cagcagcgcc gcatgagcag
    23281 gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa caacgtcagc tcaatacccg
    23341 ctttttcgca ggttttgatt aatgcatcct gtgcctgata ttgcgcccag gaagctgcca
    23401 tcactcccgc atcttttgct gagtcggaat agccaatcat caccatctgt ttgccctgaa
    23461 tcaggccacg ataccagtca atattgagca gctgggtcat gacatcgttg gcgttgttca
    23521 ggtcatcgag ggtttcaaac agcggagcaa ccggcatcgc aaacccgata cccgcttctt
    23581 tcagcagcag gtggacagcc agcacgtcgg acggcgtttt cgccatcgag atcacgtagg
    23641 cggcaatgga gccttgcggc gcttcggcaa tcacctggca ggtatcgagc acttcgcgcg
    23701 tttcggcgct tggttgccag ttgcgcggta gcagcggacg tttggagttc agttcgcgga
    23761 tcaggaacgc ctgtttgtca gcttctgacc agctctcgta gtcgccgata ccgaggtagc
    23821 gggtcagttc acccagtgct tcggtatggc gggtgctctc ctgacggata tcaatacgga
    23881 ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc gagcagatcg ccgttggcga
    23941 taatacccat gccacacgcc tgaagtgact ggtagcaagc gtagagcggt tcccacagtt
    24001 cttcgttttg tgtcagcagc ccttctggtt ttggcagttc ttcgcctttc aggcgcgctt
    24061 ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt tttcatcaga tagcgatacg
    24121 gttctgcggc accttcttcg ccaaccagcg ccagcagttc aggggtcgct tcaaccatcg
    24181 acagttcaga aaccagcacc tgaatatctt tcaggaacaa atcggtggct ttccagcggc
    24241 tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt cgggttgccg tcgcggtcgc
    24301 cgcccatcca cgaagtaaaa cggaccggaa caaattcgac gggcagtttg tagccgaggt
    24361 tctcttccag ttgttcgttc agttcgcgca ggtaatttgg tacgccttgc cacaggctgt
    24421 tttccactac ggcaaagccc catttggctt catctaccgg gcttggacgc agcttacgga
    24481 tttcatcggt atgccatgac tgggcgatta actggcgcag gcgacgcatc agctggttgt
    24541 gttcgtagtc agcgatatct ttgttatcga gctgttttaa gcaggcgttc acttccacca
    24601 ttttgtggat cagtgtacga cgggtaattt cggtagggtg agccgtgagg accagttcca
    24661 gcgacagcga ttccactgct tttttgatgg tgtcttcgct cagttccggc tggtttttca
    24721 gtttacgcag ggtgcgggcg atcacttccg ggttgctggc agcttcgcct ttcggcgaaa
    24781 tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa ctgactaaac gcacgcgcaa
    24841 cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt gagcaactcc tggcggttag
    24901 catcattgcc agcgcgtgaa gatttcgaca acttacggat agtttctacg cgttcaagaa
    24961 tgtgttctcc caacgcatcc ttgatggttt ctcccagcac tttgccgagc atactgacat
    25021 tactacgcaa tgcggaatat tgttcgttca tattacccca gacaccccat cttatcgttt
    25081 gatagccctg tatccttcac gtcgcattgg cgcgaatatg ctcgggcttt gcttttcgtc
    25141 gtcttttata aagccacgta aaagcggtga cgtcaaatgc tgcgaaatcg cttcagcaaa
    25201 cgaataaata gcaggaattt acgtcattaa attcacgacg ctttaaataa gcgtaactta
    25261 tggaaatgtt aaaaaatcgc cccaagtaac accaaaggtg taggtcggat aagatgcgca
    25321 agtatcgcat ccgacattat tgcggcactg gagtttgaca acagtgccgg atgcggcgcg
    25381 agcgccttat ccggcctacg ttttaatgcc agcaaaaatg gtgaattacc tgggttatca
    25441 gttcgcgggt gggcttgata aaccgtgttt ccagatattc atcaggttga tgagcctgat
    25501 taattgagcc aggccccaac accagcgtcg ggcataacgt ttgaataaac ggcgcttcgg
    25561 tacagtaatt caccacttcg gtttttgctc cgagcaattt ctcaaccact tcaaccagtt
    25621 gatgattcgg tgggcattca tagccaggga tcggcggatg cagctcgtcg accgtcagac
    25681 gacccggcca gcgttcgctc accggagcca atgcatcgtt gagcaaacca ttaagttcat
    25741 tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa ctcacagcaa gcgcaaatac
    25801 ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag cgtagggtat ggcacggtaa
    25861 acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa ttgcaaaata tgcccgatgg
    25921 cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg atcgctggag tgccccgact
    25981 ggccctgaat acggatggcg ttagagatat gacctttatg tgcgcgtacc ggttgtagtg
    26041 acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc ggtagtttcg gcaaaataac
    26101 gcgctccggc catactggtt tcttcatcag cagtcgccag aatgtagagc ggttttttca
    26161 gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa cgcaaaaaag cctttcatgt
    26221 cggcggtgcc taagccgtaa agcttgccgt catgctccgt cagtgtaaac ggatcacgcg
    26281 tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc cgccagcaac aagccgccag
    26341 ccccctgtcc gcaactggcc agcatattaa atttgttgcg agttcctgga acaggctgca
    26401 cttccacatt gaagcccaaa tctttaaacc agtccgccag cagagtgatt aaatctgcat
    26461 tgctttgatc gagtgcctct tccgtggcgc ttattgaagg tgtggcaatc agagcacggt
    26521 aaatctcgat aaatggcggt aatttgtttt tcattgttga cacacctctg gtcatgatag
    26581 tatcaatatt catgcagtat ttatgaataa aaatacacta acgttgagcg taataaaacc
    26641 caccagccgt aaggtgaatg ttttacgttt aacctggcaa ccagacataa gaaggtgaat
    26701 agccccgatg ttgaatacgc tgattgtggg tgccagcggc tacgctggcg cagagctagt
    26761 gacctatgta aatcgccatc cgcatatgaa cataaccgct ttgactgttt cagcgcaaag
    26821 caatgatgcg ggaaagttaa tctccgattt gcatccgcag ctaaaaggca tcgttgatct
    26881 gccgttgcag ccgatgtcgg atatcagcga gtttagccca ggggtggacg tagtgtttct
    26941 cgccaccgcc catgaagtta gccacgattt agcgccgcag tttcttgaag cgggctgcgt
    27001 ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc accttctatg aaaaatatta
    27061 cggctttacc catcaatacc cggaactgtt ggaacaggca gcctacggtc tggcggagtg
    27121 gtgcggtaat aaattaaaag aagcgaattt gattgcggtg ccgggctgtt atccgacggc
    27181 ggcacagctg gcgctgaaac cgttgattga tgccgatctt cttgacctca atcagtggcc
    27241 ggtgatcaac gccaccagcg gcgtgagcgg tgcagggcgt aaagcggcca tttcaaacag
    27301 cttttgtgaa gttagcctgc aaccgtatgg cgtctttact catcgccatc aaccagagat
    27361 cgccacacac ctcggtgctg acgttatctt caccccacat ctgggcaatt tcccgcgcgg
    27421 cattctcgaa accattacct gccgcctgaa atcgggtgtg acccaggcgc aagtcgcgca
    27481 agtgttacag caggcgtatg cccataaacc gctggtgcgg ctgtatgaca aaggcgttcc
    27541 ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc gggtttgccg ttcagggcga
    27601 gcatctgatt attgtggcga ccgaagacaa cttactgaaa ggcgcggcgg cacaagcggt
    27661 acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag tctcttattt aagggtgcaa
    27721 tgatgaatcc attaattatc aaactgggcg gcgtactgct ggatagtgaa gaggcgctgg
    27781 aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca tcagcgtccg ctggtgattg
    27841 tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg gctgaatctg ccggtgaaaa
    27901 agaaaaacgg cctgcgggtg acgcctgctg atcagataga cattatcacc ggagcactgg
    27961 cgggaacggc aaataaaacc ctgttggcat gggcgaagaa acatcagatt gcggccgtag
    28021 gtttgtttct cggtgacggc gacagcgtca aagtgaccca gcttgatgaa gagttaggtc
    28081 atgttggact ggcgcagcca ggttcgccta agcttatcaa ctccttgctg gagaacggtt
    28141 atctgccggt ggtcagctcc attggcgtaa cagacgaagg gcaactgatg aacgtcaatg
    28201 ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc ggatctgatt ttgctctccg
    28261 acgtcagcgg cattctcgac ggcaaagggc aacgcattgc cgaaatgacc gccgcgaaag
    28321 cagaacaact gattgagcag ggcattatta ctgacggcat gatagtgaaa gtgaacgcgg
    28381 cgctggatgc ggcccgcacg ctgggccgtc cggtagatat cgcctcctgg cgtcatgcgg
    28441 agcagcttcc ggcactgttt aacggtatgc cgatgggtac gcggatttta gcttaagttt
    28501 tgttggccgg aggcgcagct ttccggcatt gaatttcaaa ataaggaaac agagttatgg
    28561 cactttgggg cgggcgtttt acccaggcag cagatcaacg gttcaaacaa ttcaacgact
    28621 cactgcgctt tgattaccgt ctggcggagc aggatattgt tggctctgtg gcctggtcca
    28681 aagccctggt cacggtaggc gtgttaaccg cagaagagca ggcgcaactg gaagaggcgc
    28741 tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca aatccttgaa agcgacgccg
    28801 aagatatcca tagctgggtg gaaggcaaac tgatcgacaa agtgggccag ttaggcaaaa
    28861 agctgcatac cgggcgtagc cgtaatgatc aggtagcgac tgacctgaaa ctgtggtgca
    28921 aagataccgt tagcgagtta ctgacggcta accggcagct gcaatcggcg ctggtggaaa
    28981 ccgcacaaaa caatcaggac gcggtaatgc caggttacac tcacctgcaa cgcgcccagc
    29041 cggtgacgtt cgcgcactgg tgcctggcct atgttgagat gctggcgcgt gatgaaagcc
    29101 gtttgcagga tgcgcttaag cgtctggatg tcagcccgct aggctgtggc gcgctggcgg
    29161 gaacggccta tgaaatcgac cgtgaacagt tagcaggctg gctgggcttt gcttcggcga
    29221 cccgtaacag tctcgacagc gtttctgacc gtgaccatgt gttggaactg ctttctgctg
    29281 ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga tctgattttc tttaacaccg
    29341 gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc cggttcatca ttaatgccgc
    29401 agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa atgcggtcgg gtgcaggggg
    29461 cgttaaccgg catgatgatg acgctgaaag gtttgccgct ggcttacaac aaagatatgc
    29521 aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg gctggactgc ctgcatatgg
    29581 cggcgctggt gctggacggc attcaggtga aacgtccacg ttgccaggaa gcggctcagc
    29641 agggttacgc caacgccacc gaactggcgg attatctggt ggcgaaaggc gtaccgttcc
    29701 gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc cattcgtcag ggcaaaccgc
    29761 tggaagatct gccgctcgac gagttgcaga aattcagccc ggtgattgac gaagatgtct
    29821 atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc ggcaaaaggc ggcgtctcac
    29881 cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg gttagagtaa tttgtttata
    29941 ttgctcccct gaaaatgggg agcttaccac gcctacaaag tgcgacactt cgtaggcgga
    30001 taaggcgttt acgccacatc aggcagaaat ttgtcagcat tctggtgagc ttaaatttaa
    30061 cgcccaacca gaaccattat ttgtcacatt cctttttatc attgtgtctt ctttaccttc
//
LOCUS       DAPHDX010000049        29644 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPHDX010000049 DAPHDX010000000
VERSION     DAPHDX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.049"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(44..1873)
                     /gene="glmS"
                     /locus_tag="RH058_002105"
     CDS             complement(44..1873)
                     /gene="glmS"
                     /locus_tag="RH058_002105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV3137695.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2035..3405)
                     /gene="glmU"
                     /locus_tag="RH058_002106"
     CDS             complement(2035..3405)
                     /gene="glmU"
                     /locus_tag="RH058_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV3137696.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDSTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="RH058_002107"
     CDS             complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="RH058_002107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV3137697.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="RH058_002108"
     CDS             complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="RH058_002108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV3137698.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="RH058_002109"
     CDS             complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="RH058_002109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV3137699.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="RH058_002110"
     CDS             complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="RH058_002110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV3137700.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="RH058_002111"
     CDS             complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="RH058_002111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV3137701.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="RH058_002112"
     CDS             complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="RH058_002112"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV3137702.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="RH058_002113"
     CDS             complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="RH058_002113"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV3137703.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="RH058_002114"
     CDS             complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="RH058_002114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV3137704.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="RH058_002115"
     CDS             complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="RH058_002115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV3137705.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="RH058_002116"
     CDS             complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="RH058_002116"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV3137706.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="RH058_002117"
     CDS             complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="RH058_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV3137707.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="RH058_002118"
     CDS             complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="RH058_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV3137708.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="RH058_002119"
     CDS             complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="RH058_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV3137709.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15358..16350
                     /gene="asnA"
                     /locus_tag="RH058_002120"
     CDS             15358..16350
                     /gene="asnA"
                     /locus_tag="RH058_002120"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV3137710.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="RH058_002121"
     CDS             complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="RH058_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV3137711.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="RH058_002122"
     CDS             complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="RH058_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV3137712.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19520..21388
                     /gene="kup"
                     /locus_tag="RH058_002123"
     CDS             19520..21388
                     /gene="kup"
                     /locus_tag="RH058_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV3137713.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21555..21974
                     /gene="rbsD"
                     /locus_tag="RH058_002124"
     CDS             21555..21974
                     /gene="rbsD"
                     /locus_tag="RH058_002124"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV3137714.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21982..23487
                     /gene="rbsA"
                     /locus_tag="RH058_002125"
     CDS             21982..23487
                     /gene="rbsA"
                     /locus_tag="RH058_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV3137715.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23492..24457
                     /gene="rbsC"
                     /locus_tag="RH058_002126"
     CDS             23492..24457
                     /gene="rbsC"
                     /locus_tag="RH058_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV3137716.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24482..25372
                     /gene="rbsB"
                     /locus_tag="RH058_002127"
     CDS             24482..25372
                     /gene="rbsB"
                     /locus_tag="RH058_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV3137717.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25498..26427
                     /gene="rbsK"
                     /locus_tag="RH058_002128"
     CDS             25498..26427
                     /gene="rbsK"
                     /locus_tag="RH058_002128"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV3137718.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26431..27423
                     /gene="rbsR"
                     /locus_tag="RH058_002129"
     CDS             26431..27423
                     /gene="rbsR"
                     /locus_tag="RH058_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV3137719.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27389..28816)
                     /gene="mdtD"
                     /locus_tag="RH058_002130"
     CDS             complement(27389..28816)
                     /gene="mdtD"
                     /locus_tag="RH058_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV3137720.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28839..29531)
                     /locus_tag="RH058_002131"
     CDS             complement(28839..29531)
                     /locus_tag="RH058_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV3137721.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7118 a         7606 c         7580 g         7340 t
ORIGIN      
        1 aaagggccac cggtcggcag ccctttttta tctatgatag tgattactca accgtaaccg
       61 attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acatggtaag
      121 ccagcagctg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca
      181 tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat
      241 acaactgacc gccacgcgcg cgaacttctt caatgttgga tttcagtttt tccaacagtt
      301 cgttgttcgg tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc agcgggccgt
      361 gtttcagctc gcctgcagcg taggcttcag cgtgaatgta agagatctct ttcagtttca
      421 atgcgccttc cagcgcgatt gggtactgat cgccacggcc caggaacagc gcgtgatgtt
      481 tgtcagagaa atcttctgcc agagcttcaa tgcgtttgtc ctgagacagc atctgctcaa
      541 tacggctcgg cagcgcctgc agaccatgca cgatgtcatg ttcaatggag gcatccagac
      601 ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactga gtggtgaatg
      661 ctttagtgga tgccacgccg atttccgtac ccgcgttggt catcagcgcc agatcggatt
      721 cgcgcaccag agaagaaccc ggaacgttac agattgccag cgaaccaagg taacccagct
      781 ctttcgacag acgcaggccc gccagggtat ccgcggtttc gccagactgt gacaaggtta
      841 tcatcaggct gttacgacgc acggcagatt tgcgatagcg gaattcagag gcgatttcga
      901 cgtcgcacgg aatacctgcc agcgattcaa accagtaacg ggaaaccata ccggagttat
      961 aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt
     1021 tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt
     1081 ttatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat
     1141 cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt
     1201 tatcgaagat gtttaccgaa cggcgagtaa tttccgcaat atcgccctct tcaaggaaga
     1261 taaagcgacg ggtcaccggc aacagcgcca gctggtcaga agcgataaag ttttcgccca
     1321 tacccaggcc aatcaccagc ggactaccag aacgtgccgc cagcagggta tccgggtgac
     1381 gggagtccat gatcactgta ccgtacgcac cacgcagttg cgggatagca cgcagaacag
     1441 cctcacgcag agtaccgccc tgtttcagct cccagttcac cagatgggca attacttcgg
     1501 tgtctgtttc agaaacgaag gtatagccac gcgcttttaa ctcttcgcgc agcggttcat
     1561 ggttttcgat gatgccgtta tgcaccacca caatgtgttc agaaacatgc ggatgcgcat
     1621 tcacttctga aggttcaccg tgggtcgccc agcgagtatg agcaataccc gtaccgccat
     1681 gcagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc
     1741 gggtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt
     1801 ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa
     1861 caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg
     1921 ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg
     1981 ggagggagat tatgttatct cctcatccca tgtgaccgga ttacccggcc agaatcactt
     2041 tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa
     2101 tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc
     2161 tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat
     2221 ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac
     2281 gttatcgcca atttccgcat cgcccaggta agtcagatga ccagctttcg agcctttacc
     2341 cagacgcgct tttttcatct caacaaagtt accgacgtgt gcaccttcca gcaactcagc
     2401 accaggacgc agtcgggcaa acgggccaat agtacaggcc gctgccagat tcgcgtcttc
     2461 cacaacggta tacggactga tttcgcaatc atcgccaatc acgctgtttt taatcacgca
     2521 accggtgccg attttcacgc gatgaccgag agtcacgttg ccctcgatga taacgttagt
     2581 atcaatttca acatcgcgcc cgtgagtaag cgtaccgcgc aaatcaaaac gcgccggatc
     2641 gcgcagcata acgcctgcta acagcagttt ttcagcctgt tcggactggt aaacgcgctc
     2701 cagacgggag agttgcaggc ggttattcac accttctact tcgcttaaac gttgcggatg
     2761 aacggcgacg atttcacgcc cttcctgata cgccagcgca ataatgtcgg tgatgtagta
     2821 ttcgccctga gcattattgt tggtcagctt cgccagccag cgtttcatat ctgcgccgtt
     2881 agcgatcaga atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg tggcatcttt
     2941 gtgctcaaca atgccggtaa ctttgccatt ttcacgggtg atacgtccat aaccggtcga
     3001 atcatccagt ttcaccgtca gcagaccaat gccaccctgc ggtttagcat cacgcaggcg
     3061 ctggagtgtt tcgacagaga tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc
     3121 atcggcaaag aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct gctctgcctg
     3181 aagcacccag ttaaggttgt cgtctttcag cgcctgttta agcagatcgc cgccgtgacc
     3241 gtacaccagg tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga catgctgaac
     3301 catcgctttc ccggcaaggg tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc
     3361 tttgcctgcg gcaaggatca ctacgctcat agcattattc aacatacgcg tcctgactgt
     3421 aatttgagaa cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa
     3481 atggacagag gataaattgt tcaataacgg attatccccg ctgacgaaag tgccattttc
     3541 gaccatcgtc acgccttttg tctgcagaaa attaaggcag taccttcatt taagcgtcag
     3601 gtggatgttt ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg gtgttttata
     3661 tcagcaggat ctatgtgaac gctcttcagg acggatcacg cgtaaaaaaa agccagcctg
     3721 tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg
     3781 ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg
     3841 tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct
     3901 tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt gccaggctgc
     3961 acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg
     4021 cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt
     4081 tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct
     4141 gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa
     4201 gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg
     4261 gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg
     4321 agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga
     4381 agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca
     4441 tacccaggat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc
     4501 cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg
     4561 agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca
     4621 cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag
     4681 gtacgtatac cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct
     4741 gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc
     4801 ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca
     4861 gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa
     4921 cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga
     4981 taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta
     5041 cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca
     5101 tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga
     5161 acggacacat caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca
     5221 gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc
     5281 ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta
     5341 ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg
     5401 aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca
     5461 ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct
     5521 gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct
     5581 taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag
     5641 ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag
     5701 gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc
     5761 caggttttca accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc
     5821 cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc
     5881 atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt
     5941 aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa
     6001 catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac
     6061 ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat
     6121 tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt
     6181 gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc
     6241 cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc
     6301 aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc
     6361 ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt
     6421 gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc
     6481 aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg
     6541 aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg
     6601 ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg
     6661 aagctgccaa ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga
     6721 accagagact gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc
     6781 actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga
     6841 gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt
     6901 ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc
     6961 gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg
     7021 gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc
     7081 agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat
     7141 tcggcgttaa cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc
     7201 gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca
     7261 gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat
     7321 tcgcccattg cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg
     7381 gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg
     7441 ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg
     7501 cgctggttga tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg
     7561 atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc
     7621 tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca
     7681 gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc
     7741 acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact
     7801 ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc
     7861 tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata
     7921 caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg
     7981 ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt
     8041 tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc
     8101 tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct
     8161 gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc
     8221 atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct
     8281 acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg
     8341 agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc
     8401 agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca
     8461 gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc
     8521 tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg
     8581 cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt
     8641 tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc
     8701 cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg
     8761 cttcaatttc tgcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt
     8821 tcgcttcgtc cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt acctgggctt
     8881 ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat
     8941 gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg
     9001 ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg
     9061 cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc
     9121 tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga
     9181 cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga
     9241 gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg
     9301 aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc
     9361 atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa
     9421 cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag
     9481 aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac
     9541 attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc
     9601 accggcatac atgttaccga acagtcgcaa accaagtgaa actggtttgg acagcaggct
     9661 taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt
     9721 caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca gaatcaggat
     9781 aaatacgccc aatgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag
     9841 tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag gcagtaaatc
     9901 catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt
     9961 gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat
    10021 cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa
    10081 aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca
    10141 gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag
    10201 gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc
    10261 ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc
    10321 cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac
    10381 accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc cacccggcct
    10441 ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga
    10501 aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac
    10561 agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt
    10621 cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc
    10681 gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata
    10741 cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat
    10801 ttttccagtg catacaattg cgacttttct gctaaccctg ttcgatcatg aaaaactgta
    10861 aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg
    10921 cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta
    10981 aaaacagata ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt
    11041 taataatgtt ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt
    11101 tcactaatga cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag
    11161 agtgaacgtg gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca
    11221 gtctaaccgg tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat
    11281 caccaccaga tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac
    11341 ctgatattct tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta
    11401 gaaacggcct tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc
    11461 aaaagcgcgg ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct
    11521 ctgtactggt tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac
    11581 gcgtttacca aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg
    11641 aatgcctggc agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg
    11701 tgccaccaca atgctatcga gaatatggcg taccagcatc tcattaggat cgcggaccga
    11761 agtcaggttg tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt
    11821 tttctggtga tcggtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag
    11881 cacggtgatt acctgttctt gatgcgttgc ctggtaagcg ggtgcttacc aggcattttt
    11941 aatgcgttat gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga
    12001 gatggccgca ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ctggtttgtg
    12061 atcgttaagt ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt
    12121 cgcgggtagc agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc
    12181 gatataacct tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa
    12241 cgcaggggca aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag
    12301 cagatcttca ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc
    12361 tgcagcttcc gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat
    12421 attctcaagt ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg
    12481 accgatttca gtcaaacgca gatccgcatt atcttcgcgt agcatcagac gatattctgc
    12541 gcgcgaagta aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaactag
    12601 tacgccgaga tacgcctgag aacgtgccgg agcccaacct tctttgtcag cagacagacg
    12661 ggcagcgtta agaccggcca gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc
    12721 gttaatctga ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag
    12781 gtcgcgagga tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt
    12841 ttccatcccc tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga
    12901 gataccgttc ggataaattt cattagaggt cagtccttcc ggttcaagga agatctgatg
    12961 ctgatttctg tcggcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc
    13021 gacaccttcg atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc
    13081 atgggttttc tcgttggtat gagtgatata acacggcacc tgctggggat gctgggacgc
    13141 attgcccata aacgagaata ccggcattgg gttatcgcca tgctgttgcg ccagcacact
    13201 aaagtcgata gttcgagcat caatacgcgg tggtgtcccg gttttcagac gaccaacgcg
    13261 cagcggcagt tcacgcaaac ggcgagaaag cggaatggac ggcggatcac cagcacggcc
    13321 accgctgtaa ttatccagac cgatatgaat tttaccgtcg aggaacgtcc caacggtgag
    13381 cacgacggct ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc
    13441 gttttcgaca ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc
    13501 cagcgccgta cgtaccgcct gacggtagag cacacgatcc gcctgagctc gggtagcgcg
    13561 aaccgccggt cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc
    13621 tttcgccatc agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc
    13681 gccgatcgcc gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag
    13741 agtctgttga cccatacgcg ccgcggccat cgcggcctcg gtgcctgcat gacccccgcc
    13801 aatgatgatg acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt
    13861 atgaaaatgg attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga
    13921 cctgggatcc tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat
    13981 ctcttattag gatcgcactg ccctgtggat aacaaggatc cggcttttaa gatcaacaac
    14041 ctggaaagga tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag
    14101 aatgaggggt tatacacaac tcaaaaactg aacaacggtt gttctttgga taactaccgg
    14161 ttgatccaag cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt
    14221 gagtaaatta atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc
    14281 aagaatgttg atcttcagtg tttcgcctgt ctgttttgca ccggaatttt tgagttctgc
    14341 ctcgagttta tcgatagccc cacaaaaggt gtcatattca cgactgccaa taccgattgc
    14401 gccaaagcgg accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga
    14461 aaggttgtcc ggaatatctc cggcaccgtg ggtggagctg ataatcagcc agatccccga
    14521 ggcagataaa tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc
    14581 cagcttttca gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat
    14641 aagagtgata tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga
    14701 acgcgttggg atctacctgt ggaaaagtat gggattaaaa aagccgatta tggcttgatg
    14761 gtacgcatga tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt
    14821 gtttggatct tgttgataag tacatgctgg agagcgtcga tcgaacggca catcactttt
    14881 ataaagatgc tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc aaggctttcc
    14941 agctttgcca gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag
    15001 cctacgtcat aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc
    15061 gcctgcttca ttttctctac tcgaacgtga atcgtccccg gactgacgcc aaattgtttc
    15121 gccagttcgg cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc
    15181 agattgtcga tcagataatt ttccatagga ttttcttatg cagattgatg attcattcta
    15241 ttttagcctt cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg
    15301 catgtgtgtc ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg
    15361 aaaaccgctt acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc
    15421 caactggaag aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg
    15481 gatggcacgc aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct
    15541 ctgcctgatg cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta
    15601 gggcaacacg acttcagcgc gggcgaagga ctgtacacgc acatgaaagc ccttcgcccc
    15661 gatgaagacc gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc
    15721 gtaatgggcg acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg
    15781 gcgggaatta aagcaaccga agctgcggtt agcgaagagt ttggcctggc accgttcctg
    15841 ccggatcaga tccacttcgt acacagccag gagttactgt ctcgttatcc ggatctcgat
    15901 gccaaagggc gtgagcgggc gatagcgaaa gatcttggcg cggtattcct tgtcgggatt
    15961 ggcggcaagc tgagcgatgg tcatcgccac gacgtgcgcg caccggatta tgatgactgg
    16021 agcaccccgt cagagctggg tcatgcgggt ctgaacggcg atattctggt gtggaaccct
    16081 gtactggaag atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgttg
    16141 aagcatcagt tggcgctgac cggtgatgaa gatcgtctgg agctggagtg gcatcaggcg
    16201 ctgctgcgcg gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgacc
    16261 atgctgctgc tgcaactgcc gcatatcggc caggttcagt gtggtgtatg gccagctgct
    16321 gttcgcgaga acgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc
    16381 ggcttcgcat cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt
    16441 tgccgtgtgc cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt
    16501 tcactttact cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg
    16561 cgtcggcatc aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg
    16621 caagatcggt gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt
    16681 gtgggcctga aagctcataa cggacgatct cggtggaaaa tagcataata tagcagcgcc
    16741 ggttttcagc gagagcaatg cgcatcaagg ccaggcagaa cgctttcgca cactgttcat
    16801 taaagccgcc cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt
    16861 catcgtaatc tttatgtacc accggacgtt cgatcacttt ttcacgccac gactcaccgt
    16921 gcaggcgata ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt
    16981 ccgttatccc tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt
    17041 gcagaccatc aacctgctca ggaacagttg ccggttcgcg caccatggtg cggaaggctt
    17101 ccatctgcgc atcgttacgc ggtattgatt tggcttcccg ggaacgcccc agctgttctg
    17161 ccaggcgttt cagttccggc tgttcgttaa gaaattcacc gtatttcaca atcaactgat
    17221 agtcgccacg tttaagctga ccggcgctca tatcccacag acgaccagct gcggtattgt
    17281 tatctgcgag aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactta
    17341 acagttgttc gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca
    17401 gacttaaacg ccaacgctgg aggaaaagcg tatgtaacgc tgaagtgatc gtgctgttag
    17461 catcaaccaa ctgtcgggct tgttctgccc atggggaatt cagacgatgc agtaagtcca
    17521 ggatctgtgg tagctgcaca ataaactgcg gcgtggagag gagctggctt tgctgatagc
    17581 acatcacctc ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg
    17641 cctcacgcca gcggggaaca tcatcagtga ttgcagcctt cagtcgtggg aatttttcaa
    17701 agaagactgc cagctgcggt gaggccagca gcgcgatgat catctcttcg atcaatccct
    17761 cttcgctgac ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg
    17821 aatctgtgcg ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat
    17881 aaacagacat ttttgctgtt cgctgaataa agcgtgttgc ttgcgccagt cactttcaag
    17941 ctcttccagt tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc
    18001 cagcgtactg ccttgtaaac ttacatcgcg tacaacaaga tgttgggcgc tatccacttc
    18061 cagattcaat ttctgggcaa agccgatacc gttcagtttg ccgcgaattt caccaccttt
    18121 actcagccac tgttccagcg cgttacgctc aaaggagata tgaaccactt ccatatcatg
    18181 caattttaac ggtttttgca gcagcagagt cagagtggaa gcagtaacgt taacaggaag
    18241 ttgatactgc tgacgacggc tgaaaatgcc gcccagacga attaccgtta aggctgtttt
    18301 atcgctttgt tgctgctgga gttgcaggtg acgttgcaca atcgcgccaa ggcgagtcaa
    18361 catcccttgc tgttgccagg cgtgaccggt catcaataca tcaatttgtt gttgtatcaa
    18421 attcaggctt tgcgcgtcat accacaggca atctttcagc aaaatgagat ctaccggggc
    18481 aacagcactg cgaccgctaa aaaaggcgct ggcctgcaat aagcggatcg cttttttcca
    18541 gcgacgatcc gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa
    18601 aatcagctca aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc
    18661 atattcttca tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg
    18721 actggtcagc atggagcgga aattcgcttt atcctgcact ttatctaacc acagacgaat
    18781 cagcatgcgg tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc
    18841 cgccaccagc agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc
    18901 gttaatggcg gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag
    18961 aaagacaatt tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc
    19021 ttttagcgcc tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat
    19081 cagatattca aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact
    19141 tttggcaata cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa
    19201 acacaagcgg atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg
    19261 ggaaattctt tccgctaata aatgagggtg agccataatg aagtggcgtc ctttcgtcaa
    19321 aagttctgcg taaattgcga gtatagacgt ttcttgctgg tggctaaaat agtctcaaag
    19381 gggggggtat ttttctttga gccaggttaa tgtggccgca tttaggagta cgattttgcc
    19441 gttaatcgtg catactgtgc gcttttttgt gggccaaggg actaagcaca catttcatat
    19501 ttcaacgaaa gactagtcta tgagcactga taataagcaa tcattgcccg cgattaccct
    19561 cgcggcgatt ggagttgtct acggcgatat tggtaccagc ccgttatata cacttcgtga
    19621 atgtttgtcc ggccagtttg gttttggcgt tgaacgcgat gccgtgtttg gctttttatc
    19681 gctgatcttc tggctgctaa tctttgtggt ttccattaaa tatctcacct tcgtgatgcg
    19741 ggcagataac gccggtgagg gggggatcct gacgttgatg tcgcttgccg ggcgtaatac
    19801 gtcggcgcga accacatcaa tgctggtgat tatggggcta atcggcggca gctttttcta
    19861 tggtgaagtc gtcataacac ccgctatttc ggtgatgtca gccattgaag gtctggaaat
    19921 cgtcgccccg cagctggata cctggatagt tcccctctca attatcgttc tcactttatt
    19981 atttatgatt caaaaacatg gcacggctat ggtcggtaag ctgtttgcgc cgatcatgct
    20041 gacctggttt ttgattctgg caggactggg gttacgtagc attattgcta acccggaagt
    20101 gctgcatgcg ctgaatccaa tgtgggcggt gcatttcttc cttgaataca aaacggtttc
    20161 ttttattgca ttaggggcag tggtgttgtc gattacgggg gtcgaggcgc tgtatgctga
    20221 tatggggcac tttggtaagt tccctattcg cctggcgtgg ttcaccgtcg tattaccttc
    20281 cttgaccctt aattacttcg gtcagggagc gctgttgtta aagaacccgg aagcgattaa
    20341 gaacccgttc ttcctgttgg caccggactg ggcgctgatc ccgctgctga tcatcgccgc
    20401 actggcgacg gtaattgcct cgcaggcggt tatctctggc gtcttctcat tgacgcgtca
    20461 ggcggtacgt ctgggatatt tgtcgccgat gcgcattatt cacacctccg aaatggagtc
    20521 agggcaaatc tatattccgt ttgtgaactg gatgctctat gtcgcggtcg tgattgtgat
    20581 tgtcagcttt gagcactcca gcaacctggc ggcggcgtac gggattgcgg tgaccggaac
    20641 catggtgctg acgtctattc tctcgactac cgtggcacgt cagaactggc actggaataa
    20701 gtattttgtt gcgctgatcc tgattgcttt cctttgtgtc gatattccat tgttcaccgc
    20761 taacctcgat aaactgctct ccggcggctg gttgccattg agcctcggta ctgtgatgtt
    20821 tatcgtgatg accacctgga agagcgagcg tttccgcttg ctgcggcgga tgcatgaaca
    20881 tggtaactct ctggaagcga tgattgcttc gctggagaaa tcaccgcctg ttcgcgtgcc
    20941 cgggaccgcg gtgtatatgt cgcgtgcaat caacgtcatt ccctttgcgc tgatgcataa
    21001 ccttaaacat aacaaggtat tgcatgagcg ggtgattctg ttaactctgc gtaccgaaga
    21061 cgcgccatat gtccataatg tccgtcgggt acagattgaa caactgtcgc ccactttctg
    21121 gcgcgtggtg gcaagttatg gttggcgaga aacgccaaac gtggaagaag ttttccaccg
    21181 ctgcggtctg gaaggattaa gttgccggat gatggaaacc tccttcttta tgtcgcatga
    21241 gtcgttgatc ctcggcaaac gcccgtggta tttgcgtctg cgcggcaagc tgtacttgct
    21301 gctgcaacgt aatgcgctgc gtgcaccaga tcaatttgaa attccgccaa acagggttat
    21361 cgaactgggt actcaggtcg aaatctaacg ccagacgcct cctttctcca taagggggcg
    21421 tttttgtttt catggttaat caccatgtaa aacgtttcga ggttgatcac atttccgtaa
    21481 cgtcacgatg gttttcccaa ctcagtcagg attaaactgc gggtcagcga aacgtttcgc
    21541 tgatggagaa aaaaatgaaa aaaggcaccg ttcttaattc tgatatttca tcggtgatct
    21601 cccgtctggg acataccgat acgctggtgg tgtgtgatgc tggtttaccc atccccaaaa
    21661 gtacaacgcg tatcgatatg gcattaaccc agggtgtacc ttcttttatg caggtgctgg
    21721 gcgtcgtcac aaatgaaatg caggtcgagg ctgccattat cgcggaagag atcaaacaac
    21781 ataatccgca actccacgaa acgttgctca ctcaccttga gcagctgcaa aaacaccagg
    21841 gaaataccat tgaaattcgt tacaccacgc atgaacaatt caaacaacaa accgcagaaa
    21901 gtcaggcggt aattcgcagc ggagaatgtt ctccgtatgc gaatatcatt ctctgtgctg
    21961 gcgtgacgtt ctgaggccgt catggaagca ttacttcagc ttaaaggcat cgataaagcc
    22021 ttcccgggcg taaaagccct ctcgggcgca gcgttaaatg tctatccggg ccgcgtgatg
    22081 gcgctggtgg gcgaaaacgg cgcgggtaaa tccaccatga tgaaagtgct tactggtatc
    22141 tatgctcgcg atgccggcac gcttttatgg ctggggaaag aaacgacatt taccgggccg
    22201 aaatcttccc aggaagccgg gattgggatt atccatcagg aactgaacct gatcccgcag
    22261 ttgaccattg ccgaaaacat tttcctcggt cgtgagtttg ttaatcgctt tggcaaaatt
    22321 gactggaaaa ccatgtatgc cgaagcggat aaattgctgg ctaaacttaa cctgcgcttt
    22381 aaaagcgaca agctggtggg cgatctttcc atcggtgacc agcaaatggt tgaaatcgcc
    22441 aaagtgctga gctttgagtc gaaagtcatc attatggatg aaccgaccga tgcgctgacc
    22501 gataccgaaa ccgaatccct gttccgcgtc atacgcgagc tgaaatcgca aggccgcggt
    22561 attgtctata tctcccaccg catgaaagaa atcttcgaga tttgcgatga cgttaccgtt
    22621 tttcgtgacg ggcaatttat tgctgagcgc gaagtggcat cactgaccga agattcgctg
    22681 attgagatga tggtgggccg caagctagaa gatcaatatc cacacctgga caaagcgccg
    22741 ggagatatcc gcctgaaagt cgataatctc tgcggacctg gcgttaacga tgtctctttt
    22801 actttacgca aaggcgaaat tcttggcgtc tctggtttga tgggcgcagg tcgtaccgaa
    22861 ctgatgaaag tgctctacgg cgcactaccg cgcaccagcg gttacgtcac cctggatggg
    22921 catgaagtcg ttacccgttc accgcaggat ggcctggcaa gcggcattgt gtatatctcc
    22981 gaagaccgta aacgtgacgg tttagtgttg ggcatgtcag taaaagagaa catgtcgctg
    23041 acagcgctgc gctacttcag ccgcgctggc ggcagtttga agcatgccga tgaacagcag
    23101 gctgtgagtg atttcattcg tctgtttaat gtgaaaacac cgtcgatgga acaggcaatt
    23161 ggtctgcttt ccggtggcaa tcagcaaaaa gtggcgattg cccgcggtct gatgacacgc
    23221 cccaaagtgt tgatccttga tgaacctacc cgtggcgtag atgtcggcgc gaaaaaagag
    23281 atctatcaac tgattaacca gttcaaagcc gatggcttga gcatcattct ggtgtcatcg
    23341 gagatgccag aagtattagg catgagcgat cgcatcatcg tcatgcatga agggcatctc
    23401 agcggggaat ttactcgtga gcaggccacc caggaagtgt taatggctgc cgctgtgggc
    23461 aagcttaatc gcgtgaatca ggagtaaaaa aatgacaacc cagactgtct ctggtcgccg
    23521 ttatttcacg aaagcgtggc tgatggagca gaaatcgctt atcgctctgc tggtgctgat
    23581 cgcgattgtc tcgacgttaa gcccgaactt tttcaccatc aataacttat tcaatattct
    23641 ccagcaaacc tcggtgaacg ccattatggc ggtcgggatg acgctggtga tcctgacgtc
    23701 gggcatcgac ttgtctgtag gttctctgtt ggcgctaacc ggcgcagttg ctgcatctat
    23761 cgtcggcatt gaagtcaatg cgctggtggc tgtcgctgcc gctctcgcgt taggtgccgc
    23821 aattggtgcg gtaaccgggg tgattgtagc gaaaggtcgc gttcaggcgt ttatcgctac
    23881 gctggttatg atgcttttac tgcgcggcgt gaccatggtt tataccaacg gtagcccagt
    23941 gaataccggc tttactgaga acgccgatct gtttggctgg tttggtattg gtcgtccgct
    24001 gggcgtaccg acgccagtct ggatcatggg gattgtcttc ctcgcggcct ggtacatgct
    24061 gcatcacacg cgtctggggc gttacatcta cgcgctgggc ggcaacgaag cggcaacgcg
    24121 tctttctggt atcaacgtca ataaaatcaa aatcatcgtc tattctcttt gtggtctgct
    24181 ggcatcgctg gccgggatca ttgaagtggc gcgtctctcc tccgcacaac ccacggcggg
    24241 gactggctat gagctggatg ctattgctgc ggtggttctg ggcggtacga gtctggcggg
    24301 cggaaaaggt cgcattgttg ggacgttgat cggcgcatta attcttggct tccttaataa
    24361 tggattgaat ttgttaggtg tttcctccta ttaccagatg atcgtcaaag cggtggtgat
    24421 tttgctggcg gtgctggtag acaacaaaaa gcagtaataa cgactacagg acatcttgaa
    24481 tatgaacatg aaaaaactgg ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag
    24541 tgcgaatgcg atggcaaaag acaccatcgc gctggtggtc tccacgctta acaacccgtt
    24601 ctttgtatcg ctgaaagatg gcgcgcagaa agaggcggat aagcttggct ataacctggt
    24661 ggtgctggac tcccagaaca acccggcgaa agagctggcg aacgtgcagg acttaaccgt
    24721 tcgcggcacc aaaattctgc tgattaaccc gaccgactcc gacgcagtag gtaatgctgt
    24781 gaagatggct aaccaggcga acatcccggt tatcactctt gaccgccagg caacgaaagg
    24841 tgaagtggtg agccacattg cttctgataa cgtactgggt ggcaaaatcg ccggtgatta
    24901 catcgcgaag aaagcgggtg aaggtgccaa agttatcgag ctgcaaggca ttgctggtac
    24961 atccgcagcc cgtgaacgtg gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa
    25021 tgttcttgcc agccagccag cagattttga tcgcactaaa ggtttgaacg taatgcagaa
    25081 cctgttgacc gctcatccgg atgttcaggc tgtattcgcg cagaatgatg aaatggcgct
    25141 gggcgcgctg cgcgcactgc aaactgccgg taaatcggat gtgatggtcg tcggatttga
    25201 cggtacaccg gatggcgaaa aagcggtgaa tgatggcaaa ctagcagcga ctatcgctca
    25261 gctacccgat cagattggcg cgaaaggcgt cgaaaccgca gataaagtgc tgaaaggcga
    25321 gaaagttcag gctaagtatc cggttgatct gaaactggtt gttaagcagt agttttaatc
    25381 aggttgtatg acctgatggt gacataaata cgtcatcgac agatgaacgt gtaatataaa
    25441 gaaaagcagg gcacgcgcca ccctaacacg gtggcgcatt ttatggacat cccgaatatg
    25501 caaaacgcag gcagcctcgt tgttcttggc agcattaatg ctgaccacat tcttaatctt
    25561 caatcttttc ctactccagg cgaaaccgta accggtaacc actatcaggt tgcatttggc
    25621 ggcaaaggcg cgaatcaggc tgtggctgct gggcgtagcg gtgcgaatat cgcgtttatt
    25681 gcctgtacgg gtgatgacag cattggtgag agcgttcgcc agcagctcgc cactgataac
    25741 attgatatta ctccggtcag cgtgatcaaa ggcgaatcaa caggtgtggc gctgattttt
    25801 gttaatggcg aaggtgagaa tgtcatcggt attcatgccg gcgctaatgc tgccctttcc
    25861 ccggcgctgg tggaagcgca acgtgagcgt attgccaacg cgtcagcatt attaatgcag
    25921 ctggaatcac cactcgaaag tgtgatggca gcggcgaaaa tcgcccatca aaataagact
    25981 atcgttgcgc ttaacccggc tccggctcgc gaacttcctg acgaactgct ggcgctggtg
    26041 gacattatta cgccaaacga aacggaagca gaaaagctca ccggtattcg tgttgaaaat
    26101 gatgaagatg cagcgaaggc ggcgcaggta ctgcatgaaa aaggtatccg tactgtactg
    26161 attactttag gaagtcgtgg tgtatgggct agcgtgaatg gtgaaggtca gcgcgttcct
    26221 ggattccggg tgcaggctgt cgataccatt gctgccggag atacctttaa cggcgcgtta
    26281 atcacggcgt tgctggaaga aaaaccattg ccagaggcga ttcggtttgc ccatgctgcc
    26341 gctgcgattg ccgtaacacg taaaggcgca caaccttccg taccgtggcg tgaagagatc
    26401 gacgcatttt tagacaggca gaggtgacgc ttggctacaa tgaaagatgt tgcccgcctg
    26461 gcgggcgttt ctacctcaac agtttctcac gttatcaata aagatcgctt cgtcagtgaa
    26521 gcgattaccg ccaaagttga agcggcgatt aaagaactca attacgcgcc atcagccctg
    26581 gcgcgtagcc tcaaactcaa tcaaacacat accattggca tgttgatcac tgccagtacc
    26641 aatcctttct attcagaact ggtgcgtggc gttgaacgca gctgcttcga acgcggttat
    26701 agtctcgtcc tttgcaatac cgaaggagat gaacagcgga tgaatcgcaa tctggaaacg
    26761 ctgatgcaaa aacgcgttga tggcttgctg ttactgtgca ccgaaacgca tcaaccttcg
    26821 cgtgaaatca tgcaacgtta tccgacagtg cctactgtga tgatggactg ggctccgttc
    26881 gatggcgaca gcgatcttat tcaggataac tcgttgctgg gcggagactt agcaacgcaa
    26941 tatctgatcg ataaaggtca tacccgtatc gcctgtatta ccggtccgct ggataaaact
    27001 ccggcgcgcc tgcggttgga aggttatcgg gcggcgatga aacgtgcggg tctcaacatt
    27061 cctgatggct atgaagtcac tggtgatttt gaatttaacg gcgggtttga cgccatgcgc
    27121 caactgttat cacatccgct gcgtcctcag gccgtcttta ccggaaatga cgctatggct
    27181 gttggcgttt accaggcgtt atatcaggca gagttacagg ttccgcagga tatcgcggtg
    27241 attggctatg acgatatcga actggcaagc tttatgacgc caccattaac cactatccac
    27301 caaccgaaag atgaactggg ggagctggcg attgatgtac tcatccatcg gataacccag
    27361 ccgacccttc agcaacaacg attacaactt actccgattc tgatggaacg cggttcggct
    27421 tagatttacg ctgtcttttg atcaaattat taccatcggt tgttttcaga agcatgaaca
    27481 ttgctgctga agcaacagta ataatgccca tcgtgataaa cgtatagtgg aattgttcga
    27541 cagtcgttgt gccttccatc ccttcataaa cgcgaaggac ggccgcactt acagcaacgc
    27601 ctaaactaat cgacagttgc tgcgtgaccg ccagaacact gttaccgctg ctggcgttgt
    27661 catcggtcag atcggcaagt gtgatggtat tcatcgcggt aaattgcgtc gacatagcca
    27721 tccctaatat aaacaacggc aagatcagca tccatatcgc cattgccggt gattgcaaag
    27781 agaactgagc gatcattagc ccaataatca ccgtgatccc cactaacgta tggcgatagc
    27841 ccagacgacg taagacttgg gtaaccatcg attttgcaat aatggaacct aacgctgtcg
    27901 gtgccatcat acagccggca ataaacgcct gataaccaaa tcctacctgt aacatcaatg
    27961 gcataaggaa cggtacacaa ccggtcccca gacgggttgc aatattgcct acgataccga
    28021 tcgagaaagt gcgggtttta aataaatcta atgaaattaa tgggtttggc gtgtgtcgcg
    28081 catggagaat atagagaagc agtaacccga tgctggtgac aattaccgtc aaggcaatcc
    28141 agctggcgac aatcttttcc ccgaatagct ctattccgct tgagaagaga acaaggctga
    28201 ggccaaacag caaaaagcca gtgatatcga atctgcgtcg tgcggtggtg aaattgggca
    28261 tatgtttgcg cgcgtaaaga aggcccgcaa tacctatggg gatattgatt aaaaatatcc
    28321 agtgccaggt tgcccaggtg accagcacgc cgccaagaac ggggcctaaa attggcccca
    28381 ccagacccgg catggcgaca aaattcaata ctggaagaag ttcattacga ggataagcgc
    28441 gcagtaaggc cagccgagca acaggcatca tcattgcgcc gcctatcccc tgaataaccc
    28501 ggaagacaac cagctgtggt agcgaattag aaagtgcgca ggccagagaa cccaatgtga
    28561 acagactcac ggcaagggta aaaatgcgac gcgtaccgaa gcgatcggct agccatccgc
    28621 ttaccggaat aagcatcgcc accgtcagcg tataactgat gatggctgat tgcatcgcga
    28681 gaggagaacg attaaggcta tgagcgattg cgggtaaggc ggtattcaga atagtggcat
    28741 caagtgcctg catgaagaag gccatcgccg cgatccacgg caaacccgcc atactgcgct
    28801 tctttttatc gctcattcaa tgtcctgtta tcgggttgtc acttatcagg tgagcgtagc
    28861 agcgcctgac aagctttaaa tgccgcgtcg ccatcgcttt ggacaatcgc atcgacaatc
    28921 gcctggtgca gatccagctt tatcactgtg tcgctggtaa ttgacgtgaa gtaagtgtga
    28981 taaaccgaat ggaatagcga ggcgaatgag gtcaaaaacg gattggcgct catttcatag
    29041 atatgctcat gccaggccat atcgacttcg atccagcgtt cacggcgaaa gttctctttt
    29101 aatgccgcca tttcggccat taacgtattg agatgcgcct tctgttccgc ggtgccaacc
    29161 gttgcagcca gtaggcaagc ttgcggctcc agacagatac gcataaccag aaagtgatcg
    29221 atgacctgat gaaagttctc ttctgtcatc caccaggtaa gcaattcctg atcaagaaaa
    29281 ttccagtttg attgtggcat gacccgggta ccaattcgcg gtcgcggtaa aaccatccct
    29341 tttgccgtta acgttttgac cgcttcgcgt accgctgtac gactcactcc aaattgctcg
    29401 cccagctcaa tctcaccagg caaaatggtg ccgggttcat attcaccttt taagatgcgt
    29461 tgcgccagct tctcagccag aacatacgaa aggtttttct gtgcagctaa ctgttgtgcg
    29521 cttaaaggca ttactaatct tcctttttct ttttattcct ccttagtatg ccaccaggaa
    29581 gtgtgattac ggttgcaaaa acggcaaatt gcttgtttta tggcacatta acgggggttt
    29641 tgct
//
LOCUS       DAPHDX010000050        29583 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPHDX010000050 DAPHDX010000000
VERSION     DAPHDX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.050"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..467
                     /locus_tag="RH058_002132"
     CDS             <1..467
                     /locus_tag="RH058_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV3137722.1"
                     /translation="VCNYDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNPTDP
                     EELANIFNKKHKEHMDDRFIHLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTL
                     KKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            729..2351
                     /locus_tag="RH058_002133"
     CDS             729..2351
                     /locus_tag="RH058_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV3137723.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2717..3784
                     /locus_tag="RH058_002134"
     CDS             2717..3784
                     /locus_tag="RH058_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3137724.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3781..6516
                     /gene="rbbA"
                     /locus_tag="RH058_002135"
     CDS             3781..6516
                     /gene="rbbA"
                     /locus_tag="RH058_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV3137725.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPELLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYISLGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6516..7640
                     /locus_tag="RH058_002136"
     CDS             6516..7640
                     /locus_tag="RH058_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3137726.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7713..7988
                     /locus_tag="RH058_002137"
     CDS             7713..7988
                     /locus_tag="RH058_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV3137727.1"
                     /translation="MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7985..8344
                     /locus_tag="RH058_002138"
     CDS             7985..8344
                     /locus_tag="RH058_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV3137728.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8464..8865)
                     /gene="nikR"
                     /locus_tag="RH058_002139"
     CDS             complement(8464..8865)
                     /gene="nikR"
                     /locus_tag="RH058_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV3137729.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8871..9677)
                     /gene="nikE"
                     /locus_tag="RH058_002140"
     CDS             complement(8871..9677)
                     /gene="nikE"
                     /locus_tag="RH058_002140"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV3137730.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9674..10438)
                     /gene="nikD"
                     /locus_tag="RH058_002141"
     CDS             complement(9674..10438)
                     /gene="nikD"
                     /locus_tag="RH058_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV3137731.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(10438..11271)
                     /gene="nikC"
                     /locus_tag="RH058_002142"
     CDS             complement(10438..11271)
                     /gene="nikC"
                     /locus_tag="RH058_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV3137732.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11268..12212)
                     /gene="nikB"
                     /locus_tag="RH058_002143"
     CDS             complement(11268..12212)
                     /gene="nikB"
                     /locus_tag="RH058_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV3137733.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12212..13786)
                     /gene="nikA"
                     /locus_tag="RH058_002144"
     CDS             complement(12212..13786)
                     /gene="nikA"
                     /locus_tag="RH058_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV3137734.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13897..14484)
                     /gene="acpT"
                     /locus_tag="RH058_002145"
     CDS             complement(13897..14484)
                     /gene="acpT"
                     /locus_tag="RH058_002145"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV3137735.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14539..15588)
                     /locus_tag="RH058_002146"
     CDS             complement(14539..15588)
                     /locus_tag="RH058_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV3137736.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15720..16937
                     /locus_tag="RH058_002147"
     CDS             15720..16937
                     /locus_tag="RH058_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137737.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16941..17498)
                     /gene="dcrB"
                     /locus_tag="RH058_002148"
     CDS             complement(16941..17498)
                     /gene="dcrB"
                     /locus_tag="RH058_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV3137738.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17571..18236)
                     /locus_tag="RH058_002149"
     CDS             complement(17571..18236)
                     /locus_tag="RH058_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV3137739.1"
                     /translation="MDVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            18457..18702
                     /gene="tusA"
                     /locus_tag="RH058_002150"
     CDS             18457..18702
                     /gene="tusA"
                     /locus_tag="RH058_002150"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV3137740.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18804..21002)
                     /gene="zntA"
                     /locus_tag="RH058_002151"
     CDS             complement(18804..21002)
                     /gene="zntA"
                     /locus_tag="RH058_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV3137741.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALTIPVAESQRALVGSGIEA
                     QVNGEHVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21076..21702)
                     /locus_tag="RH058_002152"
     CDS             complement(21076..21702)
                     /locus_tag="RH058_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV3137742.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            21843..22202
                     /locus_tag="RH058_002153"
     CDS             21843..22202
                     /locus_tag="RH058_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV3137743.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22205..22474)
                     /locus_tag="RH058_002154"
     CDS             complement(22205..22474)
                     /locus_tag="RH058_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV3137744.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22464..23060)
                     /gene="rsmD"
                     /locus_tag="RH058_002155"
     CDS             complement(22464..23060)
                     /gene="rsmD"
                     /locus_tag="RH058_002155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV3137745.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23210..24706
                     /gene="ftsY"
                     /locus_tag="RH058_002156"
     CDS             23210..24706
                     /gene="ftsY"
                     /locus_tag="RH058_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV3137746.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24709..25377
                     /gene="ftsE"
                     /locus_tag="RH058_002157"
     CDS             24709..25377
                     /gene="ftsE"
                     /locus_tag="RH058_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV3137747.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            25370..26428
                     /gene="ftsX"
                     /locus_tag="RH058_002158"
     CDS             25370..26428
                     /gene="ftsX"
                     /locus_tag="RH058_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV3137748.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26673..27527
                     /gene="rpoH"
                     /locus_tag="RH058_002159"
     CDS             26673..27527
                     /gene="rpoH"
                     /locus_tag="RH058_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV3137749.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27798..28901
                     /gene="livJ"
                     /locus_tag="RH058_002160"
     CDS             27798..28901
                     /gene="livJ"
                     /locus_tag="RH058_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV3137750.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29100..29483)
                     /gene="panM"
                     /locus_tag="RH058_002161"
     CDS             complement(29100..29483)
                     /gene="panM"
                     /locus_tag="RH058_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV3137751.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7180 a         7548 c         8055 g         6800 t
ORIGIN      
        1 atgtttgtaa ttatgactca gaaaggcaag ttttaaccag tcatcacgga ataataagag
       61 atgaagaaaa aaaatgttat tgtctcggtg ctttgcaggt tccatttaat cagatgaaaa
      121 atcctactga tcctgaggaa ttagccaata tttttaataa aaaacataaa gaacacatgg
      181 atgatcgttt tatccatcta attagaagca atgctatagc atccacacct gtatatgaca
      241 attattttaa taacactact gctttcagac caaaacctga agatatcttc aaatgtggac
      301 agacgctcaa aaaaactaaa cagaaatatg gccattatgg attaggggta gatcagcatc
      361 aaaaaattga taattatacc atggggctaa attcctggaa gatagctcct aacgaacgtg
      421 gtgataaaaa aggtgtccca gggcttagtt gttttcaacc acattaaaac tccatgagtg
      481 ctgtggtata tctttttaat cctgccagta tttcataggt tttcttaatc gattgtagag
      541 gcatgttctg atgacgcctc tacaatcttt agggactttc tctgctatga gataagaaat
      601 ttcatattat gctttaaggc tgaatgattt gtgcaaaata taattatttt tccatcgaac
      661 atgaggtgta tgtcataaga ttaaatgtta actatgcatt cctcgcggtg aattatatga
      721 ggtaaaagat gaaaattgga actgtggcag gcactaacga cagtacaacg acaatcgcaa
      781 caaatgatat ggtgcaagag catgttacta acttcactaa agaactgttt gggtatattg
      841 caaatggtat aggtgatgat atatcaagta ttgccaggac aatgctcggt gaagttgtgg
      901 aaaaaattga tgattggcaa atagaacgtt tccaacaatc tattcgagat gacaaaatat
      961 catttaccat acaaacgaat cattcagaaa aatatagtat gctatcggac atgcgcgctc
     1021 atattctacg tagggataat aattatcagt ttattgtgac aattaatagt aaaaattacg
     1081 gctgctatct tgataatacc gatattaatt ggtgcagcat tgtttattta cttaataata
     1141 tgacagtaaa tgatgatgct aatgatgttg cagttactga gtcgtacaaa cccgtctggg
     1201 actggaaaat atcgcaattc aatgtatctg atattaaatt cgaaacaatg ataaaccccc
     1261 agttcgctga cagaacctat ttctccaatt gttcacctgt tgatcccaca agtaccagac
     1321 caacctattt tggcgatacc gatggctcag tcggtgcagt tttgtacgcg ctttttgcaa
     1381 cgggacattt gagaatcatg gctgaaggag aaaatttcct tagccagttg cttaatatag
     1441 aagatgaagt tctcaacgta ttgctaaggg agaactttaa tgagcaactt gacactaatg
     1501 ttaatacaat aatcagtatt ctgaacagaa gagatatagt tttagaaagt cttcagcctt
     1561 atttagttat taataaagac gctgtgacgc catgtacgtt tcttggcgac caaaccggcg
     1621 atcgatttag taatatctgt ggcgatcaat ttatcatcga tttgttaaaa cgcataatga
     1681 gtatcaatga aaatgttcat gtattagctg gaaatcatga aacgaattgc aatggtaact
     1741 atatgcagaa cttcacgcgt atgaaaccac ttgatgagga tacctacgct gggataaaag
     1801 attatcctgt atgtttctat gaccccaaat acaaaataat ggcaaatcat catgggataa
     1861 cttttgatga gcagcgaaaa cgttacatta taggaccgat aacagtctct attgatgaaa
     1921 tgaccaatgc tctcgatccg gtcgaattag cggcaattat caataaaaag catcatgcta
     1981 taattaatgg caaaaagttt aaaactagcc gggctatctc ttgtcgatcc tttaatcgtt
     2041 atttttctgt ttcgacagat tacagaccta aacttgaagc actattagca tgttcacaaa
     2101 tgttaggtat aaatcaagtt gttgcacata acggaaatgg tggaagagaa cgcataggcg
     2161 aaacggggac agttttaggt cttaacgccc gtgacagtaa acatgctgga agaatgttca
     2221 gcatgcataa ttgccaaatt aaccctggtg cagggcctga gataaccact ccctggaagt
     2281 cttaccagca tgaaaaaaac agaaatggac ttatgccttt gattagaagg cgcacaatgt
     2341 tacagctctg aaccatagac aacatgaata ttaatgttac tatctggttg agactgttga
     2401 taaaacgtaa aaaggaatgc tctgttcctg caactgaaca ggcgtgaaac gctatccaac
     2461 aggatggata ccgttttgtc agccagtttt atagatcaca cttatgagta tgcgtgtttc
     2521 atttacggcc aggaatgctg atgatgacaa cattactacc cgttttcact aagccttccc
     2581 cgttggcgct caatgctctg cgcgctggtc gtatttgccg tttccttctt atcccggatg
     2641 gaagaatccg ctgaacgtcg ttcggcggca gttgtaacac gcgtcgtttg cttacaggga
     2701 ttatggcggg tcgattatgg ataagagtaa gcgccatctg gcgtggtggg ttgtcgggtt
     2761 actggcggtg gcggctatcg tggcgtggtg gctgttgcgc ccggcaggtg tgccggaagg
     2821 ctttgctgtc agcaatgggc gcattgaagc gacggaagtg gatatcgcca gcaaaattgc
     2881 cgggcgtatt gacaccattc tggtgaaaga aggccagttt gttcgagaag gtgaagtgct
     2941 ggcgaagatg gatactcgcg tgttgcagga acagcgactg gaagccatcg cgcaaatcaa
     3001 agaagcacaa agcgccgttg ctgccgcgca ggctttgctg gagcaacgcc aaagtgaaac
     3061 tcgcgccgct cagtcgctgg ttaatcaacg gcaagctgaa ctggactccg tagccaaacg
     3121 tcatacccgt tcccgctcgc tcgcccatcg tggggctatt tctgcgcaac agttggatga
     3181 cgaccgcgcc gccgctgaga gcgcccgagc tgcgctggaa tcggcaaaag ctcaggtttc
     3241 ggcttctaaa gccgctatag aagcggcacg caccaatatc attcaggcgc aaactcgcgt
     3301 cgaagccgca caagccactg aacggcgcat tgccgcagat atcgatgaca gcgaactgaa
     3361 agccccgcgt gacggacgcg tgcagtatcg ggttgccgag ccaggcgaag tgctggcggc
     3421 aggcggtcgg gtgctgaata tggtcgatct cagcgacgtc tatatgactt tcttcctgcc
     3481 aaccgaacag gcgggcacgc tgaaactggg cggtgaagcc cggctgatcc tcgatgccgc
     3541 gccagatctg cgtattcctg caaccatcag ttttgtcgcc agtgtcgccc agttcacgcc
     3601 aaaaaccgtc gaaaccagcg atgaacggct gaaactgatg ttccgcgtca aagcgcgtat
     3661 cccaccggaa ttactccagc agcatctgga atatgtcaaa accggtttac cgggcgtagc
     3721 gtgggtgcgg gtgaatgaag aacttccgtg gcctgacgac ctcgtggtga ggttgccgca
     3781 atgacgcatc tggaactggt tcccgtcccg cctgtcgcgc aactggcggg cgtgagccag
     3841 cattatggaa aaaccgttgc gctgaacaat atcacactcg atattccggc ccgttgcatg
     3901 gtcgggctga ttggcccgga cggcgtcggg aagtcgagct tgttgtcgtt gatttccggt
     3961 gcccgcgtca ttgaacaggg caatgtgatg gtgctgggcg gcgatatgcg cgacccgaag
     4021 catcgccgcg acgtctgccc gcgcatcgcc tggatgccgc aggggctggg caaaaatctc
     4081 taccacacct tatcggtgta tgaaaatgtc gattttttcg ctcgcttgtt cggtcacgat
     4141 aaagcggagc gggaagtccg aattaatgag ctgctgacca gcaccgggtt agcaccgttt
     4201 cgcgatcgtc cggcaggtaa actctccggc gggatgaagc aaaaactggg gctgtgctgc
     4261 gcgttaatcc acgacccgga actgttgatc cttgatgagc caacaacggg ggttgacccg
     4321 ctctcccgcg cccagttctg ggatctgatc gacagtattc gccagcggca gagcaatatg
     4381 agcgtgctgg tcgccaccgc ctatatggaa gaggccgaac gcttcgactg gctggtagcg
     4441 atgaatgccg gagaagtgct ggcaactggc agcgccgaag agctgcggca gcaaacgcaa
     4501 agcgccacgc tggaagaggc gtttataaat ctgttaccgc aagcgcaacg ccaggcgcat
     4561 caggcggtag tgatcccacc gtatcaacct gaaaatgcag agattgccat cgaagcgcgc
     4621 gatctgacca tgcgttttgg ttccttcgtt gccgttgatc acgtcaattt ccgcattcca
     4681 cgcggggaga tttttggttt tcttggatcg aacggctgcg gtaaatccac caccatgaaa
     4741 atgctcaccg gactgctgcc cgccagcgaa ggtgaggcgt ggctgtttgg gcaaccggtt
     4801 gatccaaaag atatcgatac ccgccgtcgg gtgggctata tgtcgcaggc gttttcgctc
     4861 tataacgaac tcaccgtgcg gcaaaacctt gagttacatg cccgtttgtt tcacatcccg
     4921 gaagcggaaa ttcccgcgcg agtggctgaa atgagcgagc gttttaagct caacgacgtt
     4981 gaagatgttc tgccggagtt attgccgctc ggcattcgcc agcggctttc gctggcggtg
     5041 gcggtgattc atcgcccgga gatgttaatc ctcgatgaac ctacttctgg cgtcgatccg
     5101 gtggcgaggg atatgttctg gcagttgatg gtcgatctct cgcgccagga caaagtaacc
     5161 atttttatct ccacccactt tatgaacgaa gcggaacgtt gcgaccgcat ctcactgatg
     5221 cacgccggaa aagtgcttgc cagcggtaca ccgcaggaac tggttgagaa acgcggagcc
     5281 gccagtctgg aagaggcatt tatcgcctat ttgcaggaag cggcagggca gagcaacgaa
     5341 gccgaagcgc cgcccgtggt acacgacacc acccacgcgc cgcgtcaggg atttagcctg
     5401 cgccgtctgt ttagctacag ccgccgcgaa gcgctggaac tgcgacgcga tccggtacgt
     5461 tcgacgctgg cgctgatggg aacggtgatc ctgatgctga taatgggtta cggcatcagt
     5521 atggatgtgg aaaacctgcg ctttgcggtg ctcgatcgcg accagaccgt cagtagccag
     5581 gcgtggacgc tcaatctctc cggttcccgt tactttatcg aacagccgcc gctcaccagt
     5641 tatgacgagc ttgatcgtcg gatgcgtgcg ggcgatatca cggtggcgat tgagatcccg
     5701 cccaatttcg ggcgcgatat cgcgcgtggt acgcctgtgg aactcggtgt ctggatcgac
     5761 ggagcgatgc cgagccgtgc cgaaacggta aaaggttacg tgcaggccat gcaccagagc
     5821 tggttacagg atgtggcgag ccgacaatcc acacccgcca gccaaagcgg gctgatgaat
     5881 attgagacgc gctatcgcta taacccggac gtaaaaagcc tgccagcgat tgttccggcg
     5941 gtgatcccgc ttctgctgat gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg
     6001 gaaaaagagc ttgggtcgat tatcaacctt tacgtgaccc ccaccacgcg tagtgaattt
     6061 ttgcttggta aacagttgcc atacatctcg ctggggatgc tgaacttttt cctgctctgc
     6121 ggcctgtcgg tgtttgtgtt tggcgtaccg cataaaggca gtttcctgac gctcaccctg
     6181 gcggcgctgc tgtatatcat cattgccacc ggaatggggc tgctgatctc cacctttatg
     6241 aaaagccaga ttgccgccat tttcggaacg gcgattatca cgttgatccc ggcgacacag
     6301 ttttccggga tgatcgatcc agtagcttcg ctggaagggc ctggacgttg gatcggcgag
     6361 gtttacccga ccagtcattt tctgactatc gcccgcggga cgttctcgaa agcgctggat
     6421 ctgactgatt tgtggcaact ttttatcccg ttgctgatag ccatcccgct ggtgatgggc
     6481 ttaagcatcc tgctgctgaa aaaacaggag ggatgatgcg ccatttacgc aatattttta
     6541 atctgggtat caaagagttg cgcagtctgc tcggtgataa agcgatgctg acgctgattg
     6601 tcttctcgtt tacggtgtcg gtgtattcgt cagcgaccgt tacgccagga tcgttgaacc
     6661 tcgcgccgat cgccattgcc gatatggatc aatcgcagtt atcgaaccgg atcgttaaca
     6721 gcttctatcg cccgtggttt ttgccaccgg agatgatcac cgccgatgag atggatgccg
     6781 gactggacgc cgggcgttat acctttgcga taaatattcc gcctaatttt cagcgtgatg
     6841 tcctcgccgg acgccagccg gatattcagg tgaacgtcga tgccacgcgc atgagccagg
     6901 catttaccgg caatgggtat atccagaata ttatcaacgg tgaagtgaac agctttgtcg
     6961 cgcgctaccg tgataacagc gaaccgttgg tatcgctgga aacccggatg cgctttaacc
     7021 cgaaccttga tcccgcgtgg tttggcgggg tgatggcgat catcaacaac attaccatgc
     7081 tggcgattgt attgaccgga tcggcgctga tccgcgagcg tgaacacggc acggtggaac
     7141 acttgttggt gatgccgata acgccgtttg agatcatgat ggcaaaggtc tggtcgatgg
     7201 ggctggtggt gctggtggtg tcgggattat cactggtgct gatggtgaaa ggcgtgctgg
     7261 gtgtaccgat tgaaggctcg atcccgctgt ttatgctggg cgtggcgctc agtctgtttg
     7321 ccaccacgtc aatcggcatt tttatgggga cgatagcgcg ttcaatgccg caactggggc
     7381 tgctggtgat tctggtgctg ctaccgttgc aaatgctttc cggtggatcc acgccgcgcg
     7441 aaagtatgcc gcagatggtg caggacatta tgctgaccat gccgacgaca cactttgtta
     7501 gcctcgcgca ggccatcctc taccggggtg ccggattcga aatcgtctgg ccgcagttcc
     7561 tgacgctgat ggcaattggc ggcgcgtttt tcaccattgc actgctgcga ttcaggaaga
     7621 cgattgggac aatggcgtaa ggaagggaaa cttgacttca tactgtgatg tcatactatt
     7681 gatgtcataa agtgaagtca taaggacatg ccatgagaac caaggtacag gctttacgga
     7741 agaaacaaaa aaatacattg gatcaaatag ttaaaactcc tgttcctcaa gggatcaagt
     7801 ggtctgatat agagtcactg gttaaagcat taggcggaga aattaaggaa ggaagaggtt
     7861 cgcgttgcaa gttcatacta aatatgagcg ttgcgtgttt ccatcggcct catccgtcgc
     7921 cagataccga taaaggcgct gtagaaagcg tgcgtgactg gttactaagt ataggggtaa
     7981 aaccatgatc aaactgaaga cgccaaactc tatggagatt gccgggcaac ctgcagttat
     8041 cacttacgtg ccagaactga atgcatttcg tggcaaattc ctgggcttgt caggttattg
     8101 tgattttgtc tctgacagca ttcaggggct gcaaaaagaa ggggaacttt ccctgcgaga
     8161 gtatcttgag gattgtaaag ctgcgggtat tgagccgtat gcgcgtacag aaaagattaa
     8221 gacctttacg ttacgctatc cagaatcgct gagtgaacgc cttaataatg cggctgcgca
     8281 gcaacaagtc tctgttaata cctatattat tgaaacactg aatgagcgtt tgaatcattt
     8341 ataaatttta gcggcgtaca ttacgcagaa tatctgcctt tacaggatct atctatgcaa
     8401 ctgaacttaa ttttataaat taagttcagt taatatcttt tctttcctcg tttcagaaat
     8461 aactcaatct tccttcggca ggcactgcaa atgcccgtgc cgcacgccgc gctgggcgat
     8521 aacgtcatcg gcaaaatgct gcacgtcgcc catatcacct ttcagcacgg cgatttccag
     8581 acagtcgtcg tgattgatat gcacatgcag cgtggcgacg gagagatcgt ggtgatggtg
     8641 ctgggtggag acaatgcggc tggctaagtc gcgtttttcg tgttcataca cataggagag
     8701 caccgcgaaa ccttgcgtgc cgtgctgctg agtggcctcc tgggccagag cgctacgcag
     8761 aatatcgcgg atagcttcgg aacgattgtt ataaccacga cgctggctca ggctgtccag
     8821 cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt tgcatttgcg ttaaaccttt
     8881 tctgtggtgc gacggcgcac ggggaatgcg ggtaataccg cgttttgtag cacacgtccg
     8941 gcgtcagagg aaaaggttaa tttatctccc accacctggg tttcgacgat ttgtccgttg
     9001 tccatcacca tcacccgctg acagaagcgt tccaccaggc gtaagtcgtg ggtgatgaac
     9061 aggcaggcgg tgccaaactg ttgttgtagc tttttcagca ggcgaatgac gcccgcctgt
     9121 aacacgagat caaggttaga aactgcttca tccagaatca gcagcttcgg ttcgaccgcc
     9181 agcgcgcgag ccaggcagac gcgctggagc tggccgccgc ttaactgcgg tgggcgcttg
     9241 tcgagcacgc tgtcgtcgag atcgaccgcc ttcagcattt cgctggcgcg cgccagttgt
     9301 tcggattttt tcagtgatag caggtggcgc atcggttcac gcaggatctc gcgcacggtt
     9361 ttgcgcggat tcacggcgct gatggagtcc tgaaatacca tctgaatatc gcggcggaac
     9421 gctttacgct gggcgcgatt gagtttcgcc agcggttcgc cgcgccagct aatattccct
     9481 tgtgagggag actctaagcc taccagcagc cgcgcgaggg tgctttttcc gcagccgctg
     9541 cgtcccagca gggcgacggt ttcgccgctt ttcagggtca gggaaacgtt attcagcacc
     9601 gcctgatgtt gatgttttcc gctaaatcca ccgtgggcat agtgatggga aaggccgcag
     9661 acgttaagta aggtcatgat gccagctcca taccgtagag ggcgagatga gcggaaacca
     9721 ggctgcgcgt caccgtatgt ttgggggcgt taaacagcgt ttctacatcg ccctgttcga
     9781 caattttacc ttgtgacatc accgccacgt catccgccag acgcgccaca acgcccatat
     9841 catgggtgac cagcagcatt cccggcgctt gtttttgcat aatgctttcc agcagatcga
     9901 ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg ttcatcggcg atgataaacg
     9961 gtgattcaca cagcaccgcc atcgcaatca tcatgcgctg caacatgccg ccgctcatct
    10021 cgaacgggta cagcttcagc acgcgcgcgg cgttttccag ccccaccgct tctatggcag
    10081 cggtaagcgt agcgtcatcg gcgggtttcc ctaacgccag gcaggtttcc cgcgcgtggg
    10141 tgtgcatggt gtgcagtgga ttaaaggcgc tgcgcgggtt ctgcatgatg gtggcaattt
    10201 tgatgccgcg cagggcgcaa ggcgaaaccg gtttgccatc ggctaaaatt tcccccgccg
    10261 tctggcgaac gccagcgggc aaaatgccca gcgtcgcggc gcaggttaat gatttcccgc
    10321 tgccgctacc gccgactaac gccagcacgc gcccgcgttg cagggttaac gatacaccgt
    10381 gtaccagcgg ctgcgcggcc tgtagcgcga tattacgtag ttcaatctgt tgcggcatta
    10441 gtgtgcgtgc tccgtcacca gatgaggatc cagatgatcg cgcagtgcgt cacccaccag
    10501 gttaaaggcc atcacgctga taaacagcgc cagccccggc cagaacattt gcagcggctg
    10561 ggtccagata tactggcgcg cgtcgttaat catcacgccc cattcggcgg tcggcgcggt
    10621 cacaccgagg ccaaggaaag acatccccgc gacgtgcagc atcatatggc cgatatccag
    10681 cgttgccagc accagcagcg aagggatcac cgcgcctgcc agatgatcga caaacacccg
    10741 cacatggccc gcaccggaaa gccgtgacgc cagcacaaac tcgcgttggc gtagtgaaat
    10801 caccaggctg cgcaccatgc gtgcatacca cgcccagtgc gacagggcga tggcgataat
    10861 tacgttggtc agcccggtgc cgagcacgcc aaccataaag aacgacagaa tcgaggtcgg
    10921 gaaggtcata aacatatcgg cgacgcgcat ggtggcctga tcaacgcgtc cgccaatcag
    10981 tccggcgctg ccgccaatca ccagccctaa tgtcagcacc agcagcaggc aggccattac
    11041 cgaaccgagc gacacgcggg tcgccgccat cagccgcgag aaaatatcac gccctaagtg
    11101 atcggtgccc agccagtgct gcgcatccgg cgaaagcagg cgcgacggca gatcaatagc
    11161 ctgagggtca tacggcagcc accactggct ggtaagcgca atcagcgcca gcagggcgat
    11221 aatgatcagc gccaggcgta ccgaccagcg ggaagagagg aaaaagttca cgcgtgcgct
    11281 ccttcatgac gacgaatgcg cgggtccagc gcggcgttga gcaaatcgac aatcaaatta
    11341 cagaccacaa aaaccaccac catcatcagc gtaaagcact ggatcaccgg atagtcacgg
    11401 ttaaaaatcg ccgacaccgc atagcgcccg acgcccggcc aggcaaagat gttttcgata
    11461 atcatcgtcc cgccgatcag ttcgccgatg tgcatcccca cggcggtgat catcggcagc
    11521 gaggcattgc gcaggatgtg gcgacgttcg gtctgtttgt cgttcaggcc gcgcagacgc
    11581 gcccaggtga cgtgacgctg accggcgacg tccagcatac tggcgcgcag taaacgcgcg
    11641 ttaatcgcca gcgacataaa ggcaatggaa accgcaggca aaatgatgtg ctgccagccg
    11701 ccgtaaccca tcgcgggtag ccattgcaga tacaccgaaa acgccatcac cagtaaaaac
    11761 gccagccaga agttaggcat cgacacgcca agaaacgcga taaagcgcac ggcgaaatcc
    11821 ggcagacggt cgcggtggcg cgccgcccag ataccgagcg gtacggaagt gagcagaatt
    11881 aataccagcg ccgcacctgc aagttccagc gtggcgggca ggaagttcag catatcgtcc
    11941 agtaccgggc gttggctggc gaatgagata ccaaagtcaa gatgcagcgc cttccacaac
    12001 caggtgccgt actggacgta cagcggctga tccagtccca gcatggtgcg ggtagaggcc
    12061 agcatctccg gcgtcggcgg caggttagac agacgcaaat aatcgagcgc cgggtcgccg
    12121 gtaccgaggc gcagcatcag aaaaatgatc accgaggcgg cgagcaccat cgggatcagc
    12181 agcagaaagc ggcgtaatac gtaacgcaac attaaggttt caccggttta atctgttcga
    12241 acggaatttc ggtggcgatc ggcgcgtagg ggatattacc cagctccggt tttgatacca
    12301 ccatcatcga gatgtagctg ataggcagat aaaccgcctc gtcatgcaga cgggtcagaa
    12361 tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt cgccagcact tcgccgatct
    12421 ctttatcaat cagcggctta tcggctaatc cttgctgtgc ctggaagtca gcgtgcgacg
    12481 gtacgcgcat tgaactcagg aaggcgtgtg gatcgtatgg cgcgccccag gtgcggtgga
    12541 aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat actgctctct tcttcgccaa
    12601 tcagcgagac atctgcgcca atctggcgca tatcagcctg aatgatttcc gccatcgatt
    12661 tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg cagcggctga ccatttttct
    12721 cgcggatgtc tttgcccgca ggcagcgtcc aaccggcttt ttccagcaac tctttcgctt
    12781 tctgcggatc gtactggcgc ggtttcaggc cgagattagc gtaaggcaca gaaggggcaa
    12841 acagggtgtc ggcgacctgc tgggtgccat ataacgcgtt atcaatcagc gattttttgt
    12901 ttaccgcgta attaagagct tcacgtactg ccagctcgtt ggtgggggct ttggcggtat
    12961 tgagcgccag catcacggtt tcgatcggct gtgacagttg ggtgtggtaa gccggattct
    13021 ggctaaagcg ggcgaaggta tcgagcggta ataacccttc gtttccgtac agcaggtcga
    13081 tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc cgggatgacg ttaaaggtga
    13141 tctttttaat cgctggcttt tcgccccagt agttttcgtt acggacgaag acatcgtact
    13201 gattcagttt cgattcctgc aaaatccatg gtccggtgcc aatcggcgct ttaattccgt
    13261 tcatggtttc atggttttta aactgcgagg gagcgataaa acggaaagga cggggcaggg
    13321 ccagttcttg caggaaagga tagtaggcgc ttttcagggt aatttgcagc tctgttttac
    13381 tgagtgcttt aacatcaaca atctggtttg ccagctccag ccaggcgtga cgttgacggt
    13441 tatcgagcac tgcgcggaag ttttctgccg ccgcctcggc atcgaacggt tcaccgttgg
    13501 agaatttcac gtcatcacgc agggtgaagg tccaggtttt accatcttct gaatgagtcc
    13561 agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc aatggttcat
    13621 aaaccatgct ctgggcgaac atctggttag gcgtgtaaag gtgcgggttt agtggcccga
    13681 cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg ataaaagacg
    13741 cacaagccag cagcgcaaat agagtgcggc ggagtgtgga gagcatggta accccaatgg
    13801 attaaaatag atggcggaaa taagtatgac gattttaagt attcgtcata ctgattacct
    13861 gttctggatc aatagatggg cagtcgggtg ggcggatcag ttaactgaat cgatccattg
    13921 cacactgtcg gcggtgagcg taaagggagt aggggtgcag atcgccaggc ttaaattttc
    13981 aagctgacaa tggctgactg acagcgagga gtgataggtg ctgtctacgc tgacgatttg
    14041 ccaggcgctg ccgccacgct gtttaacgat ggcttctttg cgcgtccaga tgcgccagaa
    14101 catttccagt tgctgatcag gatgcacggc gtccatctca gcgtgttccc cgaggctgaa
    14161 tacggcgttc gccagccagc gccagttggc gcgcgggcga ataacttcga tatcgcagcc
    14221 gacttcgcct tcatcactca acagcagggc gatatcgtca ccgctatggc ttaagttgaa
    14281 ccatagcggc gtttccggcg caaatgcagg tttgccttgt tcgccataga tgatctccgg
    14341 tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt cgcgtcgtgg
    14401 accttgcggt gcttgctcgc gtaaacccgg tggcagtgga gctgcgctta aggtcgaaac
    14461 tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat gaaagtgcgt
    14521 atcgtatcac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcctg gccccagtaa
    14581 aatcgccagt ttgctaccgc ctttggtggt ttccatccag attttacaca cactggttaa
    14641 tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccaaatta acaatgaaag
    14701 aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggct ctaaaatatt
    14761 gccgatgacc atatggacaa ctaaaaacaa tgcgccgacc agaatacatt cgtaaatacc
    14821 attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcgc cgatattggg
    14881 cacgtagtta agcaaaaacg ccagtactgc ccacatcagc gcaaactgta cccccatcag
    14941 ctccagcccc agccagacga tgacacctgt ccataaactg agtagcgtct tcaatgcaag
    15001 atagtgcgaa acgcctttaa gtgcgcggtg taatcccgcg atgtgaatct gtggattatt
    15061 cagcgcaaaa cgcattttgt aagggacgtg gcgcacttca aacagcataa aaactacggt
    15121 catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta gcgctgtggt
    15181 gaaggtaacc actttttccg agtccatccg ctgcagcatt cgctccggcg acatatgcaa
    15241 attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct ccttattaaa
    15301 cttcggcagc atagagataa attcgttaaa cgatgccgcc agtacgccga ccagcgcagt
    15361 tagtgcgatc agcatcacca ccaccacaat cgtaatggca atggggcgtt gtactccccg
    15421 acgaataaac caggtgacga gcgggttgag gacgatggca aaaaacagcg ccagtaacag
    15481 ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca gcgaggccag
    15541 cttgagcaga atgtgcatgc ccgttttatc gggttgaggg gtttccattg gggcttcctt
    15601 gtgacttttt gtattaagtg tagcgggagt cacgccagca atattctgat tcttactgct
    15661 gattttcacg tcagcacgtg gtaaaaatga aacactgttg taaaaatgtg gtgatcctca
    15721 tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg tctgaatttg cgcattgtct
    15781 ccattgtcat gtttaacttc gccagctacc tcaccatcgg gttgccgctc gctgtattac
    15841 cgggctatgt ccatgatgtg atgggcttta gcgctttctg ggcaggattg gttatcagcc
    15901 tgcaatattt cgccaccttg ctgagccgtc ctcatgccgg acgttacgcc gatttgctgg
    15961 gccccaaaaa gattgtcgtc ttcggtttat gcggctgctt tttgagcggt ctgggatatc
    16021 tgacggcagg attaaccgcc agtctgcccg tcatcagcct gttattactt tgcctgggac
    16081 gcgtgatcct tgggattggg caaagttttg ccggaacggg atcgaccctg tggggcgttg
    16141 gcgtggttgg ctcgctgcat atcgggcgag tgatttcgtg gaacggcatt gtcacttacg
    16201 gggcgatggc gatgggtgcg ccgttaggcg tcgtgtttta tcactggggc ggcttgcagg
    16261 cgttagcgtt aatcattatg ggcgtggcgc tggtggccat tttgttggcg atcccgcgtc
    16321 cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg cgcggtgctt gggcgcgtct
    16381 ggctgtacgg tatggcactg gcactggctt ccgccggatt tggcgtcatc gccaccttta
    16441 tcacgctgtt ttatgacgct aaaggttggg acggtgcggc tttcgcgctg acgctgttta
    16501 gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg cattaaccgt atcggcggct
    16561 taaacgtagc gatgatttgc tttagcgttg agataatcgg cctgctactg gttggcgtgg
    16621 cgactatgcc gtggatggcg aaaatcggcg tcttactggc gggggcaggg ttttcgctgg
    16681 tgttcccggc attgggtgta gtggcggtaa aagcggttcc gcagcaaaat cagggggcgg
    16741 cgctggcaac ttacaccgta tttatggatt tatcgcttgg cgtgactgga ccactggctg
    16801 ggctggtgat gagctgggcg ggcgtaccgg tgatttatct ggcggcggcg ggactggtca
    16861 caatcgcgtt attactgacg tggcgattaa aaaaacggcc tccggaacac gtccctgagg
    16921 ccgcctcatc atcttaaaac ttactgaata accagcgtat taatgatgtt ttctgcggtg
    16981 gtctgcgctt tttgctgatc gtcagcgggc agcgtaattt gcatggtcag cagttgatta
    17041 cccacgttac ccagaataac ggaagagtac gccgtctggc ctttcgcgga gataatactg
    17101 tctaactgct gcattttgtg acctttcagc tcaatggctt tattggttac cacttgcagc
    17161 tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg ccagcaccgc cagatcttct
    17221 ttcggatcat cgcccatgat gacgatgact gctttctgcc cggtggcgtc ggaccagaca
    17281 tgcatgttat tggcctgcgt tcccagctta ccgctctggt cggtcatatc cgctggcagc
    17341 gagaaactta acttgccatc aagcaggttg acgggattcc cggtagcatt actttccgcg
    17401 accgaaccct gcgccgtagc gttagtgtct ttatcatcac aggccgcaag ccccataacc
    17461 agcaggccaa ttccgacata tttaaccaga ttgcgcattg acttcttcct ttcgataaac
    17521 ggccataacg gctcattcat ccatcttatc acaactctga taacgaacct ttaactcgcc
    17581 tgcaaagcgt tgatttcgga tttatctgcc agtcttttca acagcatatt gagtaatacg
    17641 ccatacattg gcaggaagaa aacgatactg attaacactt tgaaacagta atcgaccagc
    17701 gcgatttcca tccagtgttc agccataaag gcatccgggc tacgccagaa ggcaatgaag
    17761 aaaaaggcca gcgtgtcgct gacgttaccg aacagtgtgg acgctgtcgg tgccagccac
    17821 cagcggcgac tttgacgcag gcggttaaaa acgtgcacgt cgaggatttg ccccagcgcg
    17881 taggccatga aactggcggt ggcgatacgg gcgacaaaca ggttgaagtg ggcgagtgcg
    17941 ccgaatccct gccatgaacc catatagaac agcgacgaga tgacatagga gattaataac
    18001 gcagggatca ttaccgcgaa gataatgcgt cgggccagcg gtgcgccaaa aatacgcacg
    18061 gtcaggtcgg tcgcgagaaa gataaacgga aaactaaacg cgccccaggt ggtatgaaaa
    18121 ccaaaaatgg agaccggaag ctggaccaga tagttactgg aggtgatcac cagcagatga
    18181 aataacgata accagaacaa cgccttatag cgttgagttt gcgagaaaac gtccatattg
    18241 tacctttttg attaaccatt ggggtgaggg aacccaatac gtacgacacg tctgttatct
    18301 taacataata acgtgcttac ccttttttcg agccgccgca tgatactgct ttacgttgac
    18361 aatgcaatgg ttaattttca cgcaatcgtt aacctggttt gcttacggac tcacagggcg
    18421 taaactagcg ccgttttttt aagtgatgag aagaaaatga ccgatctctt ttccagccct
    18481 gaccacacac tcgacgcact tggcctgcgc tgcccggaac cggtgatgat ggtgcgcaaa
    18541 actgtgcgca atatgcagcc tggcgaaacg ttgctgatta tcgccgacga tccggccact
    18601 acccgcgata ttcctgggtt ttgtaccttt atggaacacg aactggttgc gaaagagacg
    18661 gatggactgc cttatcgtta tttgattcgt aaaggcggtt gatggggaaa gattggcttc
    18721 gatgccgcct tttcccctca ccctaaccct ctccccagag gggcgagggg accgatcgcg
    18781 ctcaatgttg cgatcggttt gccttatctc ctgcgcaaca atcttaacgc attcgctgtc
    18841 accagcaccg tcgcacccgt atctgccagc accgccagcc atagtccggt catccctaaa
    18901 agcgtggtga cgaggaagat ccctttcagt cccagcgcaa tggtgatgtt ctggcggata
    18961 ttggcgtgag tggcgcgtgc cagttcaatc atttgcacca ggccgcgcag gtggttatgg
    19021 gttaatgctg catcagcggt ttccagcgcc acgtctgtgc cactgcccat tgcaatccca
    19081 atggcggcag ctttcatcgc tggcgcgtcg ttaataccat caccgaccat cgccaggggc
    19141 gcatgttgat tcagcttggt caccgctttg actttatctt ccggcaatag gcccgcttta
    19201 aactccagcc ccagctcccc ggcgattgcc gccgctgcac gtggattatc gccggtgagg
    19261 atcactcctt tgacccccag cgcgttcagt tcactgatgg cagttgcagc atcggcgcgc
    19321 agggtatcct gcaatgcaat gatacccagc acgtcatcgt tacgtactac cagcactacc
    19381 gtttgcccgg cgctttccag ttcattaatc aaaccagcaa atgcatcagc gggatgtttc
    19441 cctgcagcgc atatcaacac gtgctcaccg ttaacctgcg cttcaatgcc agacccgacc
    19501 agcgcccgct gtgactcggc gacgggaatg gtcagtgcag caacctgtgc ttctcgcacg
    19561 atggcctgcg ccagtggatg cgtcgcgcct tgctcgaccg ccgccgccag tgtcagcagt
    19621 tcagattcac taatacccgt tgccggatga atcgcggtaa cgcgcggttt accgacggtc
    19681 agcgtaccgg ttttatcaaa cgccaccagg gtaacacgac ccagttgttc cagcgccgct
    19741 ccgcctttaa ttaacgcccc acgacgcgct gctgctgcca gcccggaggt aatcgccgcg
    19801 ggcgtggaga taactaacgc gcacgggcag ccaatcagca gcagcgtcag gcctttataa
    19861 atccactcct gccagctggc ggcaaacagc agcggcggca ccagcgttac cagcagggcg
    19921 acggccataa tcgctggcgt ataaatacgg ctgaaacggt cgataaaccg ctcaatgggg
    19981 gcgcggcgct cttcggcttc ttcgatcagt ttcagaatcc ggtcaatggc gctggcaccc
    20041 ggttctgaca gcacttccag cgttaccaga cggtctacgc tggtggctcc agcagggact
    20101 ttatcgcccg tcgcgcgctc caccggaatg gattctccgg tcagggcgct ttcatcaaaa
    20161 ctggcaaacg gtgagagcaa tttaccgtcg gcaggcaaac gcccacctgc ggcgacttca
    20221 atcacatcgc cagggcgcag gctgttaatc gccacctctt cccgctcacc gttacgcagg
    20281 cgcgtggcgg tttctggttt cagcgccatt aacgcgctga ccccttgacg tgcgcggctg
    20341 gcggcccagc cttccagtcg ttcgccaatc aaaaacagca gcaacaccat cgcagcttca
    20401 gccgttgcgc caataaacag cgcaccaata gcggctacgc tcattaacgt ttcaatggca
    20461 aagtagctgc cggatttgat caaccgtaat gcctgacgag caatcgggta cagcccaacc
    20521 agcgtggtcg cgataaacgc cagttgcccg aacggatgat tgaactgctc caaaccccag
    20581 ctgattgcca tcatcacgat aagcgtaatc agcggcagat tctctttcag gcgcgatgct
    20641 tgtggctcgt cggcggcctg ctcatcgcgc agggaatagc ccgctttttg caccgcagat
    20701 tcaacctggg cacggatgtc attgtcggca tcaaccacca gtttttcggt ggcgaacaac
    20761 acctgtacct gattcacgcc tgcaagctgg cgcacggcat tttctacctt gcgcgcacag
    20821 gcggcgcagt ccatgccgct gactttccag ctatagcggg tgccggagac gttttcagag
    20881 agagttggcg tgctggaaca tgcgccgtcg cagcaacagt cgttggcgtt ctgtaccgtg
    20941 gttagcggtt tgaacgcagc aaattgaggg gctttcttgc cgtgattgtc aggagtcgac
    21001 atggcatcct ccggttaagt tttttctcat taaccgaagg atacactctg gagtcgactc
    21061 cagagtcaag ttttatcaga gatacagcga gcggacgatc aggaagtgac cggcaaagta
    21121 gcaggccgca gcaatcgcgt tatccgcacg gaagcgtcgg cgatagtgac tacccagcca
    21181 gacaaagtta ctgataaaca gcaacgaagc gccgacaaac gcagagagcg ccggagcggt
    21241 cggacggaag aaccacagtt cacctgccag ccacaccatc accagcgtca tgccgataaa
    21301 cgtgcagata ggccaacgat actcttccag ccgcgtccag ataatcgcca gtaacagcgc
    21361 gcccagaacc agcagcacca gtggcagagg ccagaagaaa gagagcgtca tctgactggc
    21421 gaaatagatg gtgtacagca ggtgcgagag gaaaaacgcg ccgatggcgt acatcagacg
    21481 ttgacgtggc aacagggtta gcgcatcgcc cagcagtgag gcgcacagcc ccgccagcac
    21541 cagatagcta atggcgtcga acatcggcgc ttgccaggcc agtaacagca ggagaagaag
    21601 ggttaatggt ttaaataccc agcgttgcca ggttggccca cgatacgatg catccacaga
    21661 tagccatgcg gaaagacaga cagcgataaa cgaccaaagc atcttagttc cttgaattcg
    21721 ttattttttc gtaagcggaa tgcctacggt cttgcttcca gtttagtggc gagggtggac
    21781 ggatgacaac accataactg gcagggtatg cttaattccg cattttccga tgagggataa
    21841 agatgagcaa accacctctt ttctttattg ttatcattgg cttaatcatc gtcgccgcat
    21901 cgtttcgttt tatgcaacaa cgacgggaaa aagctgataa tgatatggca ccactccagc
    21961 aaaagctggt gttggtgagc aacaagcggg aaaaaccgat taacgatcgc cgttcgcgcc
    22021 agcaggaagt gactccggca ggcaccagta tgcgctatga ggcaagcttc aaaccgcaaa
    22081 gcggaggaat ggagcagacg tttcgcctcg acgcccagca gtaccacgcc ctgacagtgg
    22141 gcgataaagg tacgctgagc tataaaggaa cgcgctttgt cagctttgta ggcgaacaat
    22201 aaggttattt tttaacttta aatttcttct gccagaccag cagttcaaac acgccgaaaa
    22261 ggaaaatccg taccttttcg gcggtggtca tctgcgggcc atctttcggt aaagtggatt
    22321 tcaatagcgc cagctgcata ccatgcatca gcacggtaaa aatcagcgcc acgttaacga
    22381 aaatattcag cggacgtggg aagggatgca ccaggttgag gattaaaaat ccccaaacac
    22441 agagcattaa caaacgacca atattaatca gcatcacttt ctccttgtgc ttcgcgttga
    22501 tacagccgat aagccacctg acccgccact ttttcccgat gtaatgacca gtttgccgga
    22561 acagtgggca gaccgttttc gacttcgctt tcgacataaa tcagggcttc gtcagccagc
    22621 cagccgttat cttccagtaa atttatcgtc tcttctaaca agccacggcg gaacggtgga
    22681 tcgacaaaca cgatattatg cggtgtgcct ttttgcgcca ggaatgacat tgcgttgctg
    22741 ttcaccacgc gtgcattgcc tgcttttagt gtcgccagat tcttaattaa ctgctgagaa
    22801 accgcgcgat ccatctcaat caacgtggcc cccgcagcat agcgcgacaa cgcttccagc
    22861 cccagcgcgc cgctcccggc gaagcaatcc agacattggg cgtcaacaat gaccggagcc
    22921 agccagttaa acaacgtttc gcgtacgcgg tcggtggtgg ggcgcagacc tgggctatca
    22981 ggaaccggga gtttacggcc tcgccactgc ccgccaataa tgcggatttg gccgctgccg
    23041 gaatgattcg gttttttcat gataaattgc tcaatccgcc agataacaca tacttgcagg
    23101 cggtgatgtg aattaagtta agtgatagac tatttcatca tttttttagc tgctatgtac
    23161 atagcgttaa cgctgtgcca tgaagcaaca gcgaggagtg tagtcgcaaa tggcgaaaga
    23221 aaaaaaacgt ggcttttttt cctggctggg ctttggtcaa aaagagcaga ccccggaaaa
    23281 agagacagaa gttcagaatg aacaaacggt tgtagaagaa atcgttcagg cgcaagagcc
    23341 tgtgaaggct tctgaacaag ccgtcgaaga gcagccgcag gcgcatactg aggccgaggc
    23401 ggaaactttt gctgccgacg ttgtggaagt cactgaacag gttgctgaaa gtgaaaaagc
    23461 gcagcctgaa gcggaagtcg ttgcacaacc ggaaccggtc gtagaagaaa cgccggagcc
    23521 agtggctatc gaacgtgaag agctgccgtt gccggaagac gtcaacgccg aagaggtttc
    23581 gccagaagag tggcaggctg aagcggaaac cgtagagatt gtcgaagcgg cggaagaaga
    23641 agcggctaaa gaagaaatca ccgacgaaga gctggaagca caggcgctgg ctgccgaagc
    23701 ggcagaagaa gcggtgatag tggttcctcc ggcagaagaa gagcagccgg ttgaagaaat
    23761 tgcccaggag caggaaaaac cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct
    23821 gttaaaaacc aaagaaaatc tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat
    23881 cgacgatgat ctgtttgaag agctggaaga acagctgttg atcgccgatg tgggcgtgga
    23941 aaccacacgt aaaatcatca ccaatctgac ggaaggcgca tcccgcaagc agcttcgtga
    24001 tgccgaggcc ctctatggcc tgctgaaaga agagatgggc gagattctgg cgaaagtcga
    24061 tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtag gcgtcaacgg
    24121 tgtgggtaaa accacgacga ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc
    24181 ggtgatgctg gcggcgggcg atactttccg tgcagcagcg gttgaacagc ttcaggtctg
    24241 ggggcagcgc aacaatattc cggtgattgc ccagcatacc ggtgcggatt ccgcctctgt
    24301 tatcttcgac gccattcagg ccgctaaagc gcgtaatatc gacgtcctga ttgccgatac
    24361 cgccggacgc ctgcagaaca aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt
    24421 gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg ctgactatcg atgccagcac
    24481 cgggcagaac gcggtaagcc aggccaaact gttccatgaa gctgttggct taaccggcat
    24541 cacgctaacg aaactggacg gcacggcgaa aggcggggta attttctcgg tggctgacca
    24601 gtttggtatc cctatccgct acattggtgt cggcgaacgt attgaggatt tgcgtccgtt
    24661 taaggcggac gactttatag aggcactttt tgcccgagag gattaacaat gattcgcttt
    24721 gaacatgtca gcaaggctta tctcggtggg agacaggcgc tgcagggcgt tacgttccat
    24781 atgcagccgg gtgagatggc gtttctgacc ggtcattccg gcgcagggaa aagtaccctc
    24841 ctgaagctga tctgtgggat tgagcggccc agcgccggga aaatctggtt tagcggccat
    24901 gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt
    24961 ttccaggatc accatctact gatggaccgt actgtctacg ataacgtggc aatcccgctg
    25021 attatcgccg gtgccagcgg tgacgatatt cgtcgccggg tgtcggcggc actggataaa
    25081 gtcgggctac tggacaaagc gaagaacttc cctattcagc tttctggcgg tgaacaacag
    25141 cgtgttggca ttgcccgcgc ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg
    25201 actggtaacc tggacgacgc gttgtcggaa gggattctac gtctgtttga agagtttaac
    25261 cgcgttgggg taaccgtatt gatggcaacg cacgacatca acctgatctc gcggcgttcc
    25321 tatcgcatgc tcaccctgag cgatggtcac ttgcatggag gcgtgggcca tgaataagcg
    25381 cgatgcaatc aatcatattc ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt
    25441 cggcggctct ggcgacggcg gtcgtaacgc gccaaaacgc gcgaaatcct cgccaaaacc
    25501 ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc tatgccttcc acggcgcatt
    25561 gcaggatctg aaaagcaaac cgttcgccac gtttttaacg gtgatggtta tcgccatctc
    25621 cctgacgctg cccagcgtct gttatatggt gtacaaaaac gttaaccagg cggcgacgca
    25681 atattatccg tcaccgcaaa tcactgttta cttgcaaaaa acgctggacg atgacgccgc
    25741 agcaggcgtg gtagcacagc tacaggccga gcagggcgtg gagaaagtga actatctttc
    25801 tcgtgaagac gcactgggcg agttccgtaa ctggtctggt tttggtggtg cgctggatat
    25861 gctggaagaa aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg attttcaggg
    25921 gacggaatca ctgaatacgc tgcgtgatcg tatcacccag attaacggca ttgacgaagt
    25981 gcggatggat gacagctggt ttgcccgtct ggcggcgttg accgggctgg tcgggcgcgt
    26041 ttccgcgatg atcggcgtgt tgatggtggc agcagtgttc ctcgtcatcg gcaacagtgt
    26101 gcgtctgagc atctttgctc gccgtgactc cattaacgtg cagaaactga ttggtgcgac
    26161 ggatggtttc atcctgcgcc cgttcctgta cggaggcgca ttgctgggat tttctggcgc
    26221 attgctgtca ttaattttgt cagaaattct ggtactgcgt ttgtcatcgg cggttgcaga
    26281 agtggcacag gttttcggaa cgaagtttga tatcaatggc ttatcgttcg atgaatgcct
    26341 gctgttgctg ctggtttgct ctatgattgg ctgggtggca gcgtggcttg ccacggtaca
    26401 acatttacgc cactttacgc ctgaataata aaagcgtgtt atactctttc cctgcaatgg
    26461 gttccgtagc agggaaagag accccgttgt ctcttcccgg tatttcatct ctatgtcaca
    26521 ttttgtgcgt aatttattca caagcttgca ttgaacttgt ggataaaatc acggtctgat
    26581 aaaacagtga atgataacct cgttgctctt aagctctggc acagttgttg ctaccactga
    26641 agcgccagaa gatatcgatt gagaggattt gaatgactga caaaatgcaa agtttagctt
    26701 tagccccagt tggcaacctg gattcctaca tccgggcagc taacgcgtgg ccgatgttgt
    26761 cggctgacga ggagcgggcg ctggctgaaa agctgcatta ccatggcgat ctggaagcag
    26821 ctaaaacgct gatcctgtct cacctgcggt ttgttgttca tattgctcgt aattatgcgg
    26881 gctatggcct gccacaggcg gatttgattc aggaaggtaa catcggcctg atgaaagcag
    26941 tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc cttcgccgtc cactggatca
    27001 aagcagagat ccacgaatac gttctgcgta actggcgtat cgtcaaagtt gcgactacca
    27061 aagcgcagcg caaactgttc ttcaacctgc gtaaaactaa gcagcgtctg ggctggttta
    27121 accaggatga agtcgaaatg gtggcccgtg aactgggcgt aaccagcaaa gacgttcgtg
    27181 agatggaatc acgtatggcg gcacaggaca tgacctttga cctgtcttcc gacgacgatt
    27241 ccgacagcca gccgatggca ccggtgctct atctgcagga taaatcatct aactttgccg
    27301 acggcatcga agatgataac tgggaagagc aggcggcaaa ccgtctgacc gacgcgatgc
    27361 agggtctgga cgaacgcagc caggacatca tccgtgcgcg ctggctggac gaagacaaca
    27421 agtccacgtt gcaggaactg gctgaccgtt acggtgtttc cgccgagcgt gtgcgccagc
    27481 tggaaaagaa cgcgatgaaa aaacttcgcg ctgccattga agcgtaattt ctactactaa
    27541 gcagagaacc ctggatgaga atccggggtt tttgtttttt gggcctctac aataatcaat
    27601 tccccctccg gcaaaacgtc aatccccacg cagattgtta ataaactgtc aaaatagcta
    27661 ttccaatatc ataaaaatgg ggtatgtttt agcagagtat gctgctaaag cacgggtagt
    27721 catgcataaa acgaaataaa gtgctgaatc acaacaccac aacacacgta ataaccagaa
    27781 gaatggggat tctcaggatg aacataaagg gtaaagcgtt actggcagga tgtatcgcgc
    27841 tggcattcag caatatggct ctggcagaag atattaaagt cgcggtcgtg ggcgcaatgt
    27901 ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg cgcagagcag gcggttgcgg
    27961 atatcaacgc taaaggcggc attaaaggca acaaactgca aatcgtaaaa tatgacgatg
    28021 cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat
    28081 atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc gtctgacatc tacgaagacg
    28141 aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga gctgaccgcc cgtggctatc
    28201 agctgatcct gcgcaccacc ggcctggact ccgaccaggg gccgacggcg gcgaaatata
    28261 ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca cgacaaacag caatacggcg
    28321 aaggtctggc gcgagcggtg caggacggcc tgaagaaagg caatgctaac gtggtgttct
    28381 ttgatggtat caccgccggg gaaaaagatt tctcaacgct ggtggcccgt ctgaaaaaag
    28441 agaatatcga cttcgtttac tacggcggtt atcacccgga aatggggcaa atcctgcgtc
    28501 aggcacgcgc ggcagggctg aaaactcagt ttatggggcc ggaaggtgtg gctaacgttt
    28561 cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact
    28621 acgatcaggt tccggcgaac aaacccattg ttgacgccat caaagcgaaa aaacaggacc
    28681 caagcggcgc attcgtttgg accacctacg ccgcgctgca atctttgcag gcgggcctga
    28741 atcagtctga cgatccggct gagatcgcca aatacctgaa agcgaactcc gtggataccg
    28801 tgatgggtcc gctgacgtgg gatgagaaag gtgatctgaa aggctttgag ttcggcgtat
    28861 ttgactggca cgccaacggc acggcgaccg atgcgaagta atcattaatc ggcaactttg
    28921 ggttgccgcc aaattgctaa tatcgagtac gttgcttcat gccggatgcg gcgtaaacgc
    28981 cttatccggc ctacaagatc caaagaaatc agtaaattgc aacacacatt gtaggcctga
    29041 taagcgtagc gcatcaggca atttctcgtt ggtcatcact tttgaaatca acttgacgat
    29101 taacgcttct cccagccgcc ctgttgcgcc gtaaacccca gcgcctgcat aaacgccgtc
    29161 atcacaccgc gatcttccac gcctgcatcc gccatccacc agcatgaaac ggcaggattg
    29221 ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacggcgggt gacttcccgc
    29281 acgcgcaggg aatccagtgc tccctcggtg ccgcttaagg ttacccgcac ggccgctagc
    29341 aggcgctcgt taaaacgcgc ggcgtagatg cggtggttat cgtcaacctg taaagaggaa
    29401 ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct aaagttttct
    29461 aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt
    29521 agcgaaataa tttaatcgga ggctttctct tttttatttc ttttggcagg tgattaattt
    29581 ttt
//
LOCUS       DAPHDX010000051        28870 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPHDX010000051 DAPHDX010000000
VERSION     DAPHDX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.051"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RH058_002162"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RH058_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV3137752.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RH058_002163"
     CDS             complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RH058_002163"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV3137753.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREVFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RH058_002164"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RH058_002164"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV3137754.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="RH058_002165"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="RH058_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV3137755.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="RH058_002166"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="RH058_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV3137756.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="RH058_002167"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="RH058_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV3137757.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="RH058_002168"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="RH058_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV3137758.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="RH058_002169"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="RH058_002169"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV3137759.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="RH058_002170"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="RH058_002170"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV3137760.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9972..10337
                     /locus_tag="RH058_002171"
     CDS             9972..10337
                     /locus_tag="RH058_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV3137761.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /locus_tag="RH058_002172"
     CDS             10487..11005
                     /locus_tag="RH058_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV3137762.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="RH058_002173"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="RH058_002173"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV3137763.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQEIAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /locus_tag="RH058_002174"
     CDS             12237..12719
                     /locus_tag="RH058_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3137764.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="RH058_002175"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="RH058_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV3137765.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="RH058_002176"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="RH058_002176"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV3137766.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="RH058_002177"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="RH058_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV3137767.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="RH058_002178"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="RH058_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV3137768.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="RH058_002179"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="RH058_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV3137769.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /locus_tag="RH058_002180"
     CDS             16575..17366
                     /locus_tag="RH058_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV3137770.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /locus_tag="RH058_002181"
     CDS             17387..18673
                     /locus_tag="RH058_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV3137771.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="RH058_002182"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="RH058_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV3137772.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="RH058_002183"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="RH058_002183"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV3137773.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="RH058_002184"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="RH058_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV3137774.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="RH058_002185"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="RH058_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV3137775.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /locus_tag="RH058_002186"
     CDS             complement(22621..22911)
                     /locus_tag="RH058_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV3137776.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="RH058_002187"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="RH058_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV3137777.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="RH058_002188"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="RH058_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV3137778.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="RH058_002189"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="RH058_002189"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24729..27989)
                     /locus_tag="RH058_002190"
                     /pseudo
     CDS             complement(24729..27989)
                     /locus_tag="RH058_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(28054..28173)
                     /locus_tag="RH058_002191"
     CDS             complement(28054..28173)
                     /locus_tag="RH058_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV3137779.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(28259..28516)
                     /locus_tag="RH058_002192"
     CDS             complement(28259..28516)
                     /locus_tag="RH058_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HDV3137780.1"
                     /translation="MDARVVCKPNRLGRSMWHLVVLLEELCKRGINFRALAQSIFAQQ
                     WGDECCKSKRICDLKVIVLFLCEQKIFIRNDYVNHFILPTA"
BASE COUNT         7273 a         7637 c         6684 g         7276 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacacctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcat tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg agagggtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgctta gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agcagcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcggaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacacttc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcgtcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ccgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tagaagcgct gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcggaaa aattacaaga gattgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccacgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattaca accccttaaa
    12721 acacgagcaa acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtca
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cccttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgtttcag aagacggtta acgtcctgca
    15181 cctgcatacc gcaaccggca gcaatacggc gtttacgcga acctttgatg atttctggct
    15241 tagcgcgctc tttcatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagc acgaccgcag gcggttgcgc cgccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggacgat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcgacat cactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctaat tatcgccgcc ctgtacgcgc tgcaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcattt aaccaggaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tgctaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacctattt agcatggaaa atatcgacaa ggctgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattacca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggcgcgg taattctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggtattt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgca tgttaatgcg catgatgggc atcaaaaccg atatcgtggt
    17881 cagcggctct ttgagcaaag aagagttgcg cactatcgtg catgaatcgc gctcacaaat
    17941 ttcccgtcgc aatcaggata tgctgctgtc ggtgctcgat ctggaaaaaa tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgtgtgcg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctacgcgc cgcggacgag atctattttg tcccggaagg
    18241 tacgccgctc agcacgcagt tggtgaaatt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattattg atggcactgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta cctgaagacg atgcccgcac ggttaatggc gtcattcttg aagctctgga
    18541 agagatccct gtcgctggca cccgcgtgca tattggcgag tacgatatcg atattctcga
    18601 cgttcaggac aatatgatta agcaggtaaa agtctttcct gtaaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccctactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcgcgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcttgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaaccagc ttgaagacgg gcatgaatgt ttgcttcgtc gcgtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcagt tactcaccaa
    20821 acccgagcac caaaagttcc tgcttgatgg ctatgccaat gaaacctctc tactccagga
    20881 aatgacagca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgctg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggta ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccaa cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccaat catgattgga cggtataagg acaacaccaa
    24601 taaaaacagg aagttagcag tctcagcagg acaccgacca gacggtgaag agacataaaa
    24661 ggatacgcaa aggagccgcg gctcctggtg acatgaaagc ccacagatgt gggctttttc
    24721 gttgatggtc agaacgacca gttcacacca gctaccccgt tccacgggga ttccacaccg
    24781 gcaccatggc tataccccac cccaagatgc ccgcttaacg tactgctgaa tgaggcttta
    24841 atacctgcct ggtatattcc acgtctgccc gacaaatcat tgacgaaatt accgtcacta
    24901 ttcactttca cccggttatc atcgacaaat tctttgcgca cagccgcctt cagccacggc
    24961 tcaacttcca taccgttccc cagacgcatg ttgtaactca gcgttgcacc cagttcacga
    25021 tataaactgc gggtatcgac tgatttcgat tccatgccat tggataaatg atattcaggg
    25081 ttatcagcgg tgaacccccg ttaacgatgc atacggcgtc aggttccagt taccatcggt
    25141 aaatcgcatc ccggtttcaa tgtgaccgcc cagcccgttg ctgcgataac tgccgttggc
    25201 ggctccaccg ctgccaaccg tggagtcgcg agcttcggta cctgctaata ccaataaatt
    25261 accgccattt tccagcaaca tattggtcgc taaattgccg gaaatggaaa aagtgccgta
    25321 ctggtgagta ccgctgattt caataccgtt agccgtgctc gtctggagag cggcaccgct
    25381 gttctggacg atatctgtcg ctttgccatt atcgttaact gtcagcgtac cgccttcatt
    25441 gatctttgtt tttattgcct ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat
    25501 cgttgtctca ttcgccaccc cctcgacgat ttgttcgccg ccggtgagcg tcgtgcctgt
    25561 cgcagtggct tttgttttga cgatctcccg tccgcccgta ttgacctgtg ttttgtcaga
    25621 agaggtgtct gactccatgg ttaacacgcc gccatttgcc agcaggatat tgttcgccgc
    25681 accctgctcg atgctgaacg cgacgccatc cgcgcgtgtt cctgtgaccc gcgtcgccct
    25741 ggtggttgca accaaagcgt cctggctact ctgctgtatc cccgttgcgc tgcctttctc
    25801 ccgcacatcg agtgtgccgc cgtcattaag caccgagttt tcagccagac cgccctcatt
    25861 aactacctgt gaacacccat taataatgga accttccgct gtcccgtttg ccataatttg
    25921 ttgtaggcca gagacgatat cggtattgat tgccttacca taattctgaa cggtttgcgt
    25981 gccaccattg atgtgtgttt tctctgttga cccaccatca acaatttgtt caccactttc
    26041 gatatttgcc tcagtggcta atccatatac cgtttgcttg ccacctttga tatttgcttt
    26101 atcagaagtg gcactggcat atattgtttg ggtgccagca ctattgagta cagtgccaac
    26161 atcttttcca taaacatcca ttttgccgtt ggcattaata atcgtatcaa ctgcccggga
    26221 accagtgacg actgttaatg agccagcgtt ttccagcact acatttttag cttctgaatt
    26281 cctgatgtag aaagcatcac cataactgtt ggttccttcg ataagggttc cggaagttgt
    26341 ggaagcaatt aatgcgccgc cggattgttg ctcaacatgc ttagcctcac caccgtcctg
    26401 aacctccaga acgccaccat tattaagtct ggtggtatct gttttagcct ccttctggac
    26461 aatcagctta ccgccagcat caacggtagt atttttcgcc gaggttttag ccactaccgt
    26521 cagttcgccg gtattttcca gcacaacata attagcctcc cctccggtaa tagtgaagtg
    26581 agagagcttg ttgtatcctt caatatcagt ccctgcgccc gtgttggcaa ctaaagcact
    26641 gcccgtctcc tggttaaccc catgtgcaat accaccggta tagacaatca gcgagcctcc
    26701 ggcgctaata ttgctgccag ttgccgtacc atctttctca acaacctgcc ggctcccgga
    26761 ggatataatt gtcgtgtcag ctttcccgcc gctcttgata ttttgcgttc cgccgttgat
    26821 attggtaccc gtggcaatac catgattatt aatattctgt gtaccaccat tgattatggt
    26881 attggtcgcg tttccggtaa catccattac cccgccgtta tctattcggg tcgcatcagc
    26941 tttagcattc gttaaaactg acattgttcc tttatcttta ataatcgtct tgtttgccga
    27001 accatatgcg tttatgtcta aatgaccacc gttttccagc aacacattgt ctgccacgtg
    27061 attgtggatg gagaatgcac cttcactatt cgtaccgctc accgtcgtac cgttagtgtt
    27121 tgtttttaaa attgcaccat cgtgctgggt aacatttgtt gccgtaccac cactaacatc
    27181 aagcacgcca ccggaataaa cttcaataac atccgaggag ctggtgtaat caacaatttg
    27241 cgtaccacca gaatagatct gagtattttt gccgttgact tattattcag agactgagtt
    27301 ccaccttcaa tcgtcgtgtc cagcgcatgg ctttcatata ctctttgctc accgccattt
    27361 ttaatggtgg ttgttcctac tgtgctctgt tcaacatact gtcgtccacc atttatgatt
    27421 gtgttcgttg caatacttcc ttgcgtgatg tcctgataac cggacttatt tatcgttgta
    27481 ccatcaacat gcccctgagt tgttactttc tgccctccac catcaatgat ggttccattc
    27541 gcagtccccc cacttatgtg tgaattacca cccttaattg tcgttccatt gctgatacca
    27601 cctgtataaa cgtcctgatt gccactgtcg attatcgtac cggtggaaat gcccccgtca
    27661 tgaattgtct gtcttcctcc gttaatggtt gtattattag cctgccccac aaaattgtta
    27721 tgacttccta catcttgata tccacccgat tcaataagac ttccattaga taccccgcca
    27781 tgaacattct gctggccatg gttgataata tgagtgttat ttgttgtacc tcgttcatct
    27841 actctttggc tgccatctac agtctcatcg tttaccacac caataacatc aggagtgaag
    27901 gccgtcatcc caggcggggc atatatcaag gcagatatca ataaggaaag tactgagcgg
    27961 cgacaataat ggggactggt cctgttcata aatttcatcc tctgaaaagt gaatactgag
    28021 tagcgtttaa gcgaccttag ctctgctgca acatcagccc acaggcacca gaccagggga
    28081 ttcatcctga agagacagcg caagtgtatt gtgttcaccg ctcatcaaag acatcatgat
    28141 gaaatgatga tattccacat aagaaagaag cattttttaa acgcagtgcg ctgaagtatg
    28201 gttggataaa aaagtcaatc tattcaggga atacgggggt attcttttct ttcgacaatc
    28261 aggccgtcgg caaaataaaa tgatttacat aatcgtttct gatgaatatc ttctgctcac
    28321 ataaaaataa cacaataact ttgagatcgc agattctttt acttttacag cattcgtccc
    28381 cccattgttg ggcaaatata gattgggcca gagcacgaaa gttaatacca cgtttgcaca
    28441 gctcctccaa cagcacgaca agatgccaca tactgcgccc cagtcggttc ggtttacaga
    28501 ctacccgtgc gtccaccgcc gataatgtcc tgagcagttt tttcagtccg gacctcctgc
    28561 cacggtttta cgttgaagag gtaaccctga gcacgcagtt catcagtcag gcgtggtgta
    28621 ccgtaaccct attattgatg ggtaagatca aaaaaacttt caggcagcta aggaaagttg
    28681 aaccagacat tagaataaat atttcaacca attacagcac caattcagac accgccagct
    28741 caacaaataa atcaaggggt tacgtgaaag cgtagcccct ttttctttgg tagtggcagc
    28801 aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca tattgtttca
    28861 atctacgtta
//
LOCUS       DAPHDX010000052        28256 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPHDX010000052 DAPHDX010000000
VERSION     DAPHDX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.052"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH058_002193"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH058_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3137781.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="RH058_002194"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="RH058_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV3137782.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="RH058_002195"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="RH058_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV3137783.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="RH058_002196"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="RH058_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV3137784.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="RH058_002197"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="RH058_002197"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV3137785.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="RH058_002198"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="RH058_002198"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV3137786.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="RH058_002199"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="RH058_002199"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV3137787.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="RH058_002200"
     CDS             complement(4598..5320)
                     /locus_tag="RH058_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3137788.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="RH058_002201"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="RH058_002201"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3137789.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="RH058_002202"
     CDS             6520..6738
                     /locus_tag="RH058_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV3137790.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="RH058_002203"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="RH058_002203"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV3137791.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="RH058_002204"
     CDS             complement(7472..7672)
                     /locus_tag="RH058_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV3137792.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="RH058_002205"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="RH058_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV3137793.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="RH058_002206"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="RH058_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV3137794.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="RH058_002207"
     CDS             10169..12082
                     /locus_tag="RH058_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3137795.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="RH058_002208"
     CDS             12082..13104
                     /locus_tag="RH058_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3137796.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="RH058_002209"
     CDS             13098..13316
                     /locus_tag="RH058_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV3137797.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="RH058_002210"
     CDS             13370..14239
                     /locus_tag="RH058_002210"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV3137798.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="RH058_002211"
     CDS             complement(14294..14698)
                     /locus_tag="RH058_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV3137799.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="RH058_002212"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="RH058_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV3137800.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="RH058_002213"
     CDS             15683..17773
                     /locus_tag="RH058_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3137801.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="RH058_002214"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="RH058_002214"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV3137802.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="RH058_002215"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="RH058_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV3137803.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /locus_tag="RH058_002216"
     CDS             complement(19741..20343)
                     /locus_tag="RH058_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV3137804.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="RH058_002217"
     CDS             complement(20333..20500)
                     /locus_tag="RH058_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV3137805.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="RH058_002218"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="RH058_002218"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV3137806.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="RH058_002219"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="RH058_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV3137807.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="RH058_002220"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="RH058_002220"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV3137808.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="RH058_002221"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="RH058_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV3137809.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="RH058_002222"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="RH058_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV3137810.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cobA"
                     /locus_tag="RH058_002223"
     CDS             26709..28082
                     /gene="cobA"
                     /locus_tag="RH058_002223"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV3137811.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6830 a         7577 c         7148 g         6701 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcatcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggtcag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc gactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 acacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaggaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgctc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 cgtatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacaac gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccac cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggattac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaatgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aacccagggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgctgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttttc
    10621 cgggggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggtaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc aggccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagttgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgtt
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg
    12301 cgtttagtgg tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gagctggaag ttgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggaa cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaagga ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctct cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactacgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggctg aatcaggctg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacgta gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgtt
//
LOCUS       DAPHDX010000053        28239 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPHDX010000053 DAPHDX010000000
VERSION     DAPHDX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.053"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            262..1128
                     /gene="folD"
                     /locus_tag="RH058_002224"
     CDS             262..1128
                     /gene="folD"
                     /locus_tag="RH058_002224"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV3137812.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1130..1342
                     /gene="ybcJ"
                     /locus_tag="RH058_002225"
     CDS             1130..1342
                     /gene="ybcJ"
                     /locus_tag="RH058_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV3137813.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1450..1971
                     /locus_tag="RH058_002226"
     CDS             1450..1971
                     /locus_tag="RH058_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3137814.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2007..3392)
                     /gene="cysS"
                     /locus_tag="RH058_002227"
     CDS             complement(2007..3392)
                     /gene="cysS"
                     /locus_tag="RH058_002227"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV3137815.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3566..4060
                     /gene="ppiB"
                     /locus_tag="RH058_002228"
     CDS             3566..4060
                     /gene="ppiB"
                     /locus_tag="RH058_002228"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV3137816.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4063..4785
                     /gene="lpxH"
                     /locus_tag="RH058_002229"
     CDS             4063..4785
                     /gene="lpxH"
                     /locus_tag="RH058_002229"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV3137817.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4903..5412
                     /gene="purE"
                     /locus_tag="RH058_002230"
     CDS             4903..5412
                     /gene="purE"
                     /locus_tag="RH058_002230"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV3137818.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5409..6476
                     /gene="purK"
                     /locus_tag="RH058_002231"
     CDS             5409..6476
                     /gene="purK"
                     /locus_tag="RH058_002231"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV3137819.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQN
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(6681..7574)
                     /locus_tag="RH058_002232"
     CDS             complement(6681..7574)
                     /locus_tag="RH058_002232"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV3137820.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7571..8386)
                     /locus_tag="RH058_002233"
     CDS             complement(7571..8386)
                     /locus_tag="RH058_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV3137821.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8397..9656)
                     /locus_tag="RH058_002234"
     CDS             complement(8397..9656)
                     /locus_tag="RH058_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV3137822.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRHVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9666..11333)
                     /locus_tag="RH058_002235"
     CDS             complement(9666..11333)
                     /locus_tag="RH058_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV3137823.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11650..12699
                     /gene="allD"
                     /locus_tag="RH058_002236"
     CDS             11650..12699
                     /gene="allD"
                     /locus_tag="RH058_002236"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV3137824.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12721..13956
                     /gene="allC"
                     /locus_tag="RH058_002237"
     CDS             12721..13956
                     /gene="allC"
                     /locus_tag="RH058_002237"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV3137825.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYSAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13967..14752
                     /gene="allE"
                     /locus_tag="RH058_002238"
     CDS             13967..14752
                     /gene="allE"
                     /locus_tag="RH058_002238"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV3137826.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGSNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14881..16026)
                     /gene="glxK"
                     /locus_tag="RH058_002239"
     CDS             complement(14881..16026)
                     /gene="glxK"
                     /locus_tag="RH058_002239"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV3137827.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16048..17349)
                     /locus_tag="RH058_002240"
     CDS             complement(16048..17349)
                     /locus_tag="RH058_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV3137828.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGQWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17406..18767)
                     /gene="allB"
                     /locus_tag="RH058_002241"
     CDS             complement(17406..18767)
                     /gene="allB"
                     /locus_tag="RH058_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV3137829.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18827..20281)
                     /locus_tag="RH058_002242"
     CDS             complement(18827..20281)
                     /locus_tag="RH058_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV3137830.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20451..21329)
                     /gene="glxR"
                     /locus_tag="RH058_002243"
     CDS             complement(20451..21329)
                     /gene="glxR"
                     /locus_tag="RH058_002243"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV3137831.1"
                     /translation="MKLGFIGLGIMGTPMAVNLARAGHQLHVTTIGPVAEELLALGAV
                     NVETARQVTESSDIIFIMVPDTPQVEEVLFGENGCAKTSLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21428..22204)
                     /gene="hyi"
                     /locus_tag="RH058_002244"
     CDS             complement(21428..22204)
                     /gene="hyi"
                     /locus_tag="RH058_002244"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV3137832.1"
                     /translation="MLRFSANLSMLFQEYDFLERFDKAAQCGFRAVEFMFPYDYDIEV
                     LQHKLSSNNLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAVAIRYARALGNKKI
                     NCLVGKTPVGVSAEQVHTTLVENLRYAANMLAKEGILLLIEPLNHFDMPGFHLTGTQQ
                     ALALIADIGSNNIKIQYDIYHMQRMEGELAQTMTTWANKIGHLQIADNPRRGEPGTGE
                     INYDFLFDLIEKSDYDGWVGCEYKPLTTTEAGLSWINPYR"
     gene            complement(22217..23998)
                     /gene="gcl"
                     /locus_tag="RH058_002245"
     CDS             complement(22217..23998)
                     /gene="gcl"
                     /locus_tag="RH058_002245"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV3137833.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMNQYRVPVVVEIILERVTNISMGNE
                     LDNVMEFEDIADNAADAPTETCFMQYK"
     gene            complement(24088..24903)
                     /gene="allR"
                     /locus_tag="RH058_002246"
     CDS             complement(24088..24903)
                     /gene="allR"
                     /locus_tag="RH058_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV3137834.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24981..25463)
                     /gene="allA"
                     /locus_tag="RH058_002247"
     CDS             complement(24981..25463)
                     /gene="allA"
                     /locus_tag="RH058_002247"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV3137835.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25693..26619
                     /gene="allS"
                     /locus_tag="RH058_002248"
     CDS             25693..26619
                     /gene="allS"
                     /locus_tag="RH058_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV3137836.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26688..27782
                     /gene="mnmH"
                     /locus_tag="RH058_002249"
     CDS             26688..27782
                     /gene="mnmH"
                     /locus_tag="RH058_002249"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV3137837.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27898..28239
                     /locus_tag="RH058_002250"
                     /pseudo
     CDS             27898..28239
                     /locus_tag="RH058_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6929 a         7669 c         7074 g         6567 t
ORIGIN      
        1 gaaaattctt tttatattgt caggtatttc ttaaattatc ttaatcctta aacaaggaaa
       61 taaatcagtt ccagatttac aacgccatca tggacgaaaa atgaagcttt caatctcagc
      121 gacctcagcg acggtgcgcc taaccttcgc aagaggtcgc ttcacgcgat aaatctgaaa
      181 cgaaacctga cagcgcgccc cgcttctgac aaaataggcg catccccttc gacctacgta
      241 acagatggaa tcctctctct gatggcagca aagattattg acggtaaaac gattgcgcag
      301 caggtgcgct ctgaagttgc tcaaaaagtt caggcgcgta ttgcagccgg actgcgggca
      361 ccaggactgg ccgttgtgct ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc
      421 aaacgcaagg cttgtgaaga agtcgggttc gtctcccgct cttatgacct cccggaaacc
      481 accagcgaag cggagctgct ggagcttatc gatacgctga atgccgacaa caccatcgat
      541 ggcattctgg ttcaactgcc gttaccggcg ggtattgata acgtcaaagt gctggaacgt
      601 attcatccgg acaaagacgt ggacggtttc catccttaca acgtcggtcg tctgtgccag
      661 cgcgcgccgc gtctgcgtcc ctgcaccccg cgcggtatcg tcacgctgct tgagcgttac
      721 aacattgata ccttcggcct caacgccgtg gtgattggcg catcgaatat cgtcggtcgc
      781 ccaatgagca tggaactgtt gctcgcaggt tgcaccacta ccgtgactca ccgcttcact
      841 aaaaatctgc gtcatcacgt agaaaatgcc gatctgttga tcgttgccgt tggcaagcca
      901 ggctttattc ccggtgactg gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac
      961 cgtctggaaa atggcaaagt tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc
     1021 tcatacatta cgcctgttcc cggcggcgtt ggcccgatga cggttgccac gctgattgaa
     1081 aacacgctac aggcgtgcgt tgaatatcat gatccacagg atgagtaaca tggcgacatt
     1141 ttctttaggt aaacatccgc acgttgagct gtgcgacttg ctgaaactgg aaggctggag
     1201 cgaaagcggc gcgcaggcga aaatcgcgat tgccgaaggc caggtgaaag tcgacggtgc
     1261 ggttgaaacg cgcaaacgct gcaaaatcgt cgccggtcag acagtgagtt ttgcaggtca
     1321 cagcgtacag gttgttgcct gatccctttc ccggcctgta tcaacaggcc ggttctcttt
     1381 tcagactttt gccctctacg ccccgttcat ccatgctgat agcatcgaca ttttgccgaa
     1441 gcgaccatca tgccaaccgt tattacgcac gccgccgttc ccctatgcat tggtttaggg
     1501 ttgggttcga aagtcatccc tccgcgtctg ttatttgccg gaatcatcct ggcgatgctg
     1561 cccgacgccg acgtattgtc gtttaaattt ggcgttgctt acggcaatgt ttttggtcat
     1621 cgcgggttta cccattcgct ggtgtttgcg tttgttgtcc cgctgttatg tgtgctgatt
     1681 ggacgccgat ggttcagggc tgggctgatt cgctgctggc tgtttttaac cgtctcgttg
     1741 ctatcgcaca gcttgctgga ttcggtaacc actggcggta agggcgttgg ctggctgtgg
     1801 ccgtggtcag atgaacgctt tttcgctccc tggcaggtga ttaaagtcgc gccgtttgca
     1861 ttgtctcgtt acaccacgcc gtacgggcat caggtgataa tttctgaatt gatgtgggtg
     1921 tggttgccgg ggatggtgct tatgggaatg ttgtggtggc gcaggcgata accggatgcg
     1981 aaaactcgca tccggcaaca gcgcaattac ttacgacgcc aggtggtccc ttgcgggcca
     2041 tcttccagca cgatccccat ctcgttaaga cggtcacgcg ccgcatctgc cgccgcccag
     2101 tctttcgctt tacgggcatc cagacgctgt tgaattaacg cttcaatctc agccacttcg
     2161 ctgtcgtctg cctgcgcgcc gctttgcagg aacgcttccg gttcttgctc cagcaggccc
     2221 aatacagcgg aaagtttacg caggtgagat gccattgcat tcgccgctgc catatcttct
     2281 gctttcagac ggtttacttc acgcgccata tcaaacagta cggaataggc ttccggggtg
     2341 ttgaaatcgt cgtccatcgc ttcaataaag cgcgcttcaa acgcttcgcc accggcaggc
     2401 gcaacggttt tatctgtgcc gcgcagcgca gtgtagagac gctccagcgc cgcacgcgct
     2461 tgcttcaggt tctcttcgct gtagttcaac tggctgcgat agtggcccga catcaggaag
     2521 taacgcacgg tttccgcgtc gtagtatttc agcacatcgc gcacggtaaa gaagttaccc
     2581 agcgatttgg acatcttctc gcggtcaacc atcaccatcc ccgagtgcat ccagtagttc
     2641 acatactggc catcatgggc acaggtggac tgcgcgattt cgttctcgtg gtgcgggaac
     2701 atcaggtctg aaccgccgcc gtggatatca aagtggttac ccagctgctt gcagttcatt
     2761 gccgaacatt caatgtgcca gcctggacgg cccgcgcccc acggagacgg ccagctcggt
     2821 tcgccctctt tcgacatctt ccacagaacg aagtccatcg ggttgcgttt gtcatcaacc
     2881 acgtcaacgc gcgcgccagc ctgcagctgg tcgagatcct gacgcgacag cacgccataa
     2941 gttggatcgg tcgggacgtc gaacatcacg tcgccgttgt ccgccacata agcgtgacct
     3001 ttggcgatca gttgttcagt gagttcaata atttctgcga tatggtgcgt cgcgcgcggc
     3061 tccatatccg ggcgcagaat gttcaaagca tcaaaatctt tgtgcatttc ggcgatcatg
     3121 cgatccacca gcgccacaaa gctttcgcca ttttcattgg cgcgtttgat gattttgtcg
     3181 tcgatatcgg taatgttgcg cacatacttc agtttatagc cgaggaaacg cagatagcgc
     3241 gcaaccacgt caaaagcaac aaaggtacgc ccgtgaccga tatgacagag atcgtaaacg
     3301 gtgattccac acacgtacat gccgacttcc ccggcgtgaa taggcttaaa ttcctctttt
     3361 tggcgtgtca gagtattgaa gatttttagc atcgaagatt ccgtttagac atgtgtgggt
     3421 aattgagttg cgtataatac ccatatttcc cgcgcgaatc agcatacatt gcatgatgat
     3481 ccgatcgtgc ggttatgcta taacaccacc ctatatatga cccgaactgg gttgaagcac
     3541 caatcaaacg gaacaggatg caaaaatggt tactttccac accaatcacg gcgatattgt
     3601 catcaaaact tttgacgata aagcacctga aacagttaaa aacttcctgg actactgccg
     3661 cgaaggtttt tacaacaaca ccattttcca ccgtgttatc aacggcttta tgattcaggg
     3721 cggcggtttt gaaccgggca tgaaacaaaa agccaccaaa gaaccgatca aaaacgaagc
     3781 caacaacggc ctgaaaaata cccgtggtac gctggcaatg gcacgtactc aggctccgca
     3841 ctctgcaact gcacagttct tcatcaacgt ggttgataac gacttcctga acttctctgg
     3901 cgaaagcctg caaggttggg gctactgcgt gtttgctgaa gtggttgacg gcatggacgt
     3961 ggtagacaaa atcaaaggtg ttgcaaccgg tcgtagcggt atgcaccagg acgtgccaaa
     4021 agaagacgtt atcattgaaa gcgtgaccgt tagcgagtaa tcgtggcgac actctttatt
     4081 gcagatcttc atctctgcgt ggaagaaccg gcgatcaccg ccggttttct gcgtttttta
     4141 gcgggggaag cccgcaaggc cgacgcgctg tatattcttg gcgatctgtt tgaagcatgg
     4201 attggcgacg acgatcccaa cccactccat cgccagatgg cggcagcgat caaagcggtg
     4261 tccgattccg gcgttccctg ttatttcatt catggcaacc gtgattttct gctcggcaaa
     4321 cgctttgccc gtgaaagcgg catgacatta ttgccggaag aaaaggtgct cgaactttat
     4381 ggtcgccggg tgttgattat gcatggcgac acgctgtgca ccgatgacgc gggttatcag
     4441 gcttttcgcg ccaaagtcca caaaccctgg ctgcaaacgc tattcctcgc cctgccgttg
     4501 tttgtgcgca aacgcattgc cgcgcgaatg cgagcgaaca gtaaagaagc caacagcagt
     4561 aaatcgctgg cgatcatgga cgttaaccaa aacgcggtag tcagtgcgat ggaaaaacat
     4621 caggtgcaat ggctgatcca cgggcatacc catcgcccgg cggtacatga acttatcgcc
     4681 aaccagcaac ctgcttttcg cgtggtactc ggtgcctggc atacagaagg ttcgatggtg
     4741 aaagtcacgg cggatgacgt tgagctgatt cattttccgt tttaaaaaac ccgcaacttt
     4801 gctgatttca cagccacgca accgttttcc ttgctctctt tccgtgctat tctctgtgcc
     4861 ctctaaagcc gagagttgtg caccacagga gttttaagac gcatgtcttc ccgcaataat
     4921 ccggcgcgtg tcgccatcgt gatggggtcc aaaagcgact gggctaccat gcagttcgcc
     4981 gccgaaatct tcgaaatcct gaatgtcccg caccacgttg aagtggtttc tgctcaccgc
     5041 acccccgata aactgttcag cttcgccgaa agcgccgaag agaacggtta tcaggtgatt
     5101 attgcgggcg caggcggcgc agcgcacctg ccaggcatga ttgccgccaa aacgctggtg
     5161 ccggtgctgg gcgtgccagt acagagcgcc gcactgagcg gtgtcgatag cctctactcc
     5221 atcgtacaaa tgccgcgcgg cattccggtg ggtacgctgg cgattggtaa agctggcgcg
     5281 gcaaacgcgg cattactggc agcacaaatt cttgcgactc atgataaaga actgcaccag
     5341 cgtctgaatg actggcgcaa agcccagacc gacgaagtgc tggaaaaccc ggacccgcga
     5401 ggtgcggcat gaaacaggtt tgcgtcctcg gtaacgggca gttaggccgt atgctgcgtc
     5461 aggcaggtga accgttaggc attgctgtct ggccggtcgg gctggacgct gaaccggcgg
     5521 cggtgccttt tcaacaaaac gtgattaccg ctgagatcga acgctggccg gaaaccgcat
     5581 taacccgcga gctggcgcgt catccggcct ttgtgaaccg cgatgtgttc ccgattattg
     5641 ccgaccgtct gactcagaag cagcttttcg ataagctcca cctgccgacc gcaccgtggc
     5701 agttacttgc cgatcgcagc gagtggcctg cggtgtttga gcgtttaggt gaactggcga
     5761 ttgttaagcg tcgcactggt ggctatgacg gtcgcggtca atggcgttta cgtgccaatg
     5821 aaaccgaaca gttaccggca gagtgttacg gcgaatgtat tgtcgagcag ggcattaact
     5881 tctctggtga agtgtcgctg gttggcgcac gcggatttga tggcagcacc gtgttttatc
     5941 cgctgacgca taacctgcat caggacggta ttttgcgcac cagcgtcgct tttccgcagg
     6001 ccaatgcgca gcagcaagcg caagccgaag agatgctgtc ggcgattatg caggagctgg
     6061 gctatgtggg cgtgatggcg atggagtgtt ttgtcacccc gcaaggtctg ctgatcaacg
     6121 aactggcacc gcgtgtgcat aacagcggtc actggacaca aaacggtgcc agcatcagcc
     6181 agtttgagct gcatctgcgg gcgattaccg atctgccgtt accgcaaccg gtagtgaata
     6241 gtccgtcggt gatgatcaac ctgattggta gcgatgtgaa ttatgactgg ctgaaactgc
     6301 cgctggtgca tctgcactgg tacgacaaag aagtccgtcc ggggcgtaaa gtggggcatc
     6361 tgaatttgac cgacagcgac acatcgcgtc tgactgcgac gctggaagcc ttaatcccgc
     6421 tgctgccgcc ggaatatgcc agcggcgtga tttgggcgca gagtaagttc ggttaactgg
     6481 tgctctattc ttgccggatg cggcgtaaac gccttatccg gcctaccgat ccggtaccca
     6541 ttgtaggcct gataagatgc gtcaagcatc gcatcaggca ttgtgcacca attgccggat
     6601 gcggcaccgg ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca tggtgcaccg
     6661 attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc cgccagcgtc
     6721 tcttcaattc gcgataacgc cccaatccac gcgggtttac cacggcttct gacataacca
     6781 cttaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg cgctaactca
     6841 tccggtgtgg catggcgaat ggcgcgttgc tgcggcgttc cccagttttc atataccgca
     6901 tcagcatcgg tgaggatcac cagcccatcg gcgttaatct gctcggcaag caaggcagcg
     6961 gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac aggcacaccg
     7021 ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat ggcttcgctg
     7081 tcgagaattt tacgcggttg cggagacgcc accacccggc gcaaatattt accatcacgt
     7141 ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg ataaaccgga
     7201 ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc aatgcgcgtc
     7261 agcaccgtcg tcacgggcgg catctgcggc tgtgcgctca aactctgcgc cagcatatag
     7321 ccaatcatcc cctgactttc tgccaccagc acatccagcg gatacggttc tacctctttc
     7381 cacgccagat tctgcaatgc cagcagcccc acctgcggcc cgttgccgtg aacaatcgcc
     7441 agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat attgcgatat
     7501 tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc aagagctaca
     7561 accagtgttt tcatggtttt ccttgtaata attgttgacc aagccagaac cccagcagtg
     7621 tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca gcggtacgtt
     7681 tcgggcaact cagagcatga acaaagtgca ggagtggcga ggcgaaatat ccttgcgcgg
     7741 cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgcagattg tcggaacagg
     7801 caaaaaactg gcgaccggag cgcgcatcta aagcgccata ataccaggcc gccagcagca
     7861 ttccgctcag cgtgtcatca tgactcggcg ttaatccggg gcctttaccc agccagtgcc
     7921 gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac tggcgtaact
     7981 cagcaggcag cggatcgctt gccgccaacg ccagtggccc gaaaagcccg gtttcctccg
     8041 cgcgttgcat ccatgcagct gcaagcggtt gaggatgcgc aggcggcgta atacgcagca
     8101 aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct tgagccacaa
     8161 cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc gcgcggcgaa
     8221 gcacccatcc tccggggccg aaaccactac cctgacgatg cagcgttaac agttcgccgc
     8281 tttccgtcat caggttaatc gcccgtcgcc acactcccgc taaccgccag gactggcgat
     8341 aattcggtgc actacggctg gcaagcagag gatggatgat cgtcattgcg cgttcctcaa
     8401 ccaatcccca tgctttcagc cagtgcttcc agcgcctgtt caaaacacgc cagcggtgcc
     8461 cgcacagtgc ctgcgccaat ctgcccgatc cccgcctctt tatgggcgat accggtattg
     8521 atgagtggcg taatgccggt ttctaccacg tgacgaatgt ccagtcccag gcacgcgccc
     8581 tgaaaatccc agcctgggat ctgcaactgc atattgcgct caaggtaaat ttccgccatc
     8641 tcttcagata ccgctctggc cgcttccatg ccacccgcac cgacaaagcg cgttacgcca
     8701 ggcgctgcga tcattgccgc acctccgata ccaaaggttt cggtaatcgc gctatcgccc
     8761 atatccgggt tcgcctgctc ctgcgagaag ccggtgaaaa acagaccttg cggagtgttt
     8821 acaggcgcag taaaccagcg ttcgcccagc ccgcttaccc gaatcccgaa catattgccg
     8881 ttgcgggtca ttgccgtgac gatgctgcct gcgcggatca tcgcgccagc atccatcgcc
     8941 gccttgcagt aagccatcgc gaggttgagg aagaactgat cggtcacgct gaggaaatcc
     9001 atcacttcgg cgatgtgctg tttatcatga tcgaggcgag caatttgtgg ggccagcgca
     9061 cgcattaaca gtgctgagga agcaatattg cgttgatgga actcatcgcc catggtaatg
     9121 ccctgcgcca tcatcgccgt gagatcgata ccgtgctcca tgcgccccag cgccgcgctt
     9181 aatactggca ttaacacatc gcgcatccag cgatggcgag tcaggacatc ttcgccgtaa
     9241 gcgccaaaac gcatcacttt gccgatacct tcgttgaggt tgcagtacgc ccggttgccg
     9301 tcggtcacgt tctcgaccac cagcatcggc atactggcag aagtaatgcc gcccattggc
     9361 ccgacggcat tcacatggtg acaaggaatg aagttcactt ccccctgctc cagcattgcc
     9421 agtgcctgcg cttcatcttt cgcccagcct tcaaacagac atgcacccac acatgcccct
     9481 ttcatgggtc cggtcatctc ctgccagcgc attggcggcc cggcgtgaag cagtgttttg
     9541 ccctggttta gttcgctgat aagtgaagaa gccggttgca catccagcca gtgtggacga
     9601 gcgcgacgaa tttgttcgat aaccgcagca ttggcttgcg ccactgatgt aaacatggga
     9661 accccttatt gcaaacgttc taataaacga gccagttttt tattgccgcc ggcgactggc
     9721 gaccattggt aatgcacaac cggtttgctg gcgctttgta gctccagcgc aaagctgcgt
     9781 aatccgatgt taatcacggc gacgttttcc agtaatgacg gtgtgtgttg ctgtgtggca
     9841 gacgagagcg gatgaattaa cgctgccgcc agcaaggtgg cttccggtag cgaactcact
     9901 accgcgatcc ccgcatcttc cagcgtggcg atttgctgcg agcggcattg cgggtcacgt
     9961 tcggtacctg tcaccgtggc aatggcgtac agcggttggc tgtctgaacg cgcggcacag
    10021 gctttttgcc aggcgctcac cagcgaggca gcaggatcgg cggtcgcacc gaagccaatc
    10081 acgacatcaa gcagcaacac gcgcacttgc ggtttagcgc cgagatcggc aattaactgg
    10141 ttgcgtaagg ctgggtcgat catcggatgg ggacgcccga cggtgtagaa atcatcgccc
    10201 agatcaatga tctggtgacc atcggcgtcc agcatcatgc cgtgatgatg ggtatcgtcg
    10261 gcttccacgc caaggtgccc ggcaagtaat cccgccgctt cggcagccag cgtaccgccg
    10321 gtatacaaac cgcaaataaa tccgctgctg gcaggcgtta tcgcgttacg tcgcgccgta
    10381 acgcgtgaaa gcaggcaagc caggcgtgcg gcctcatcca gcgaggaggc aaaccagaca
    10441 ttctcgtcgc gggccaccgc cggggtataa cctaaaaaca gcgccaccgt cggtttgccg
    10501 gttgctttca tggcattaac aattttcaga cgcacagctt cggcaggcgg ttttgaaaca
    10561 aatgccagca cttcgctttt ctcgtctgca ctgagcattt ccagcgccgt cagcgcactg
    10621 atgccgccca cttcacggct gaggtcgcgc ccgccaaggc caatcgcgtg agtaattccc
    10681 tcccctgcca gcgcaatctg tgagcacagc tcctgaatcc cggtaccgga agcgccaatg
    10741 acgccaatgt tgccttccgg catcacgtta gcaaaagcca gcggcgtggc ggcaatcatc
    10801 gacgtaccgc agtccggccc catcaccagc aagccttttt ctcgcgcgcg ggttttaagt
    10861 tggatttcat cttccagcgt gacgttatcg gagaacatca tcacgttgag gttgcgatcc
    10921 agcgcctgat tcgccagctc cgccgcatac tcgccagcca ctgaaatcag cgccagattg
    10981 gcatcggata atttctgaca ggcactgtcc cagcgacgca cctgcgtcaa ggactggctg
    11041 ctgcctgacc cctgcgccag ttgttttagc gcctcttcaa gctgctgcat aatcgcctgc
    11101 gcgatccccg catccgccgc ttcgctacga attgccacgc aaatatcgtt cggcgtggcg
    11161 ttattaaaat cgtcatgcca gaaacctgtg gtatctaata acgctttatt ggcgggcgta
    11221 cccatcatta cggaaacatc atcaacattt tctgattcgc tgagttttcg tgaaataatc
    11281 attaaactga ccgaatcctg aaaacaccct tttttaataa aggcgtggat cataacaact
    11341 ccttaaaaat tgcacccatg gcaattgcac gagcagtgtt aatgtggcga ttacggtatc
    11401 gatgacaacg agtaaataag gtgatgaata tcacacaggg attattaaca aaagaattca
    11461 aataaccgcc atgctattac gtgttatttt ttaataagcg tggttgtttt aaaaaccagg
    11521 ttaaaagcga gcggattatc aaataaattc taactatatt tttttgcctg tctggatcac
    11581 ataatccaga tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa
    11641 ggaagacaga tgaaaatcag tcgggaaaca ctccaccagc taattgagaa taaactctgc
    11701 caggctgggt taaaacgtga gcacgctgca accgtggctg aagtattggt ttacgccgat
    11761 gccagaggga tccactctca tggcgcggtg cgcgtggaat actacgcgga acgcatttca
    11821 aaaggcggca ccaaccgcga accggagttt cgtcttgagg aaaccgggcc gtgctcggca
    11881 attttacatg ccgacaatgc cgccggacag gtcgcggcga aaatgggtat ggaacatgcc
    11941 atcaaaaccg cccagcaaaa tggcgttgcg gtggtcggta tcagccggat gggtcacagc
    12001 ggcgcaatct cttattttgt gcagcaggca gcccgcgccg gattaattgg tatttcgatg
    12061 tgccagtccg atccaatggt ggtgccgttt ggcggcgcgg aaatttacta cggtactaac
    12121 ccactggcct ttgccgcgcc gggagaaggc gacgagatcc ttacctttga tatggcgact
    12181 accgtacagg catggggaaa agtgctcgac gcccgctcgc gtaatatgtc tatcccggat
    12241 acctgggcgg tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg
    12301 ctccccgccg ccgggccaaa agggtatggc ctgatgatga tgattgacgt cctctcaggc
    12361 gtcttactcg gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacatgcc
    12421 gggcgtaatt tggggcaatt acatatagtt attaatccga actttttctc ctccagcgaa
    12481 ttattccgtc aacatcttag ccagaccatg cgcgaattaa atgccattac ccccgcgccc
    12541 ggttttaatc aggtttatta tcccggacag gatcaggata ttaaacaacg caaagccgcc
    12601 gtcgaaggca tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat
    12661 aacacgtcat acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt
    12721 atgattacac atttccgtca agccatagaa gaaacgctac cctggctctc ctcttttggc
    12781 gctgacccag cgggtgggat gacccgttta ctttattcgc cggaatggct ggaaacccag
    12841 cagcaattta aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga tgaagtgggg
    12901 aatttatacg gtcgcctgag tggcaccgaa tatccacagg aagtggttct gagcggttcg
    12961 catatcgata ccgtggttaa cggcggtaac ctcgacgggc aattcggcgc gctggcggcg
    13021 tggctggcaa ttgactggtt gaaaacgcaa tacagcgcgc cgttacgtac ggtcgaagtg
    13081 gtggcgatgg cagaagaaga aggcagccgc ttcccgtatg tcttctgggg cagtaaaaat
    13141 atctttgggc tggcgaaccc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt
    13201 tttgtcgatg cgatgaaggc ttgcggattt actctgccga acgccccact aactccgcgt
    13261 caggatatta aagcctttgt tgaactgcat attgaacagg gctgtgtgct ggaaagtaat
    13321 ggtcaatcaa ttggcgtggt gaatgcaatt gtcggacagc gtcgctatac ggtgacgctg
    13381 aacggcgaat caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga tacggtttac
    13441 gctttcagtc gcatttgcca tcagtcgatc gaaaaagcga aaaagatggg cgatccgctg
    13501 gttctgacct ttggcaaagt agagccgcgt ccgaatacgg tgaatgtggt accgggtaaa
    13561 accacgttca ccattgattg tcgtcatacc gacgctgccg tgctgcgcga tttcacccaa
    13621 cagttagaaa acgacatgcg ggcgatttgc gatgaaatgg acattggtat tgatatcgat
    13681 ttatggatgg acgaagaacc cgtgccgatg aataaggagc tggtcgccac cctgacagaa
    13741 ttgtgtgaaa gtgaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg
    13801 caaattttcg cgcctcgcgt accaacctgc atgattttca ttcccagcat caatgggatc
    13861 agccataacc cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg
    13921 gcactcatgc tttatcaact tgcctggcag aaataaggag tcataaatgg gatatttaaa
    13981 taacgtcacc ggttaccgcg atgatttact ggctaaccgt gcgattgtta aacacggtaa
    14041 tttcgcgctg ttaaccccgg atggtctggt aaaaaatatt attccgggct ttgaaaattg
    14101 tgacgcgaca atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac
    14161 actgcatcaa aacggtagca accaacaggg cttcggtggc gaaggcattg aaacgttcct
    14221 gtatgtgatc tctggaaata tcactgccaa agccgaaggc aaaacatttg ccttaagcga
    14281 aggtggctat ctttattgcc caccaggctc cttaatgacg tttgttaacg cccaggccga
    14341 agacagccaa atctttttat ataagcgccg ctatgttccg gtagaaggcc atgcaccgtg
    14401 gctggtttct ggcaatgcca gcgaactgga acgcattcac tatgaaggta tggatgatgt
    14461 gatcctgctg gattttctgc ccaaagagtt aggttttgat atgaacatgc atatcctctc
    14521 ttttgcacca ggtgccagcc acggttatat cgaaacacat gttcaggaac acggtgccta
    14581 tattctttcc ggtcaggggg tttataacct cgacaataac tggatcccgg tgaaaaaagg
    14641 cgattacatc tttatgggcg cttattcttt acaggctggt tatggcgtag ggcgtggtga
    14701 agcgttcagc tatatttact cgaaagattg taaccgcgac gtcgagattt aagtcatacc
    14761 actcgcctga ttatttttag ctatgttgga ttttgccacg gtttataccg tggctttttt
    14821 tgtagtaaat tcaggtaaaa aaagatgcga tataaatcgc atccgcttta aaggtaaggg
    14881 tcagttttta attccctgac ctattttaat ggcgcaggca atatttcgtg cgctgttgaa
    14941 gagattggtt tcaccgctgg cgagcacttc ggctaaaggt gccagacgtg gcaaaatgct
    15001 gaataccgca tcaatgccgt actggtgcac cacttccacg ccatcaccca atacgccagc
    15061 aatcccaatc accggtacat taaactgctt cgccaccgac gccacaccca gcggcgcttt
    15121 accgcctgcc gtttgcgagt cgatgcgccc ttccccggta atcaccagtg ctgcgccctg
    15181 cactgcctgc gcaagattga ccgcattcaa cacaatctca atacctggtt taatatccgc
    15241 attgagaaat accgccgccg caatacccat accgcccgca gcgccgccgc ccgccatctg
    15301 gcagacgttg atttcagttt gctgttgtaa aacacgggcg taattttgca gcccctgttc
    15361 cagctcttcg accatttccg gcgttgcccc tttttgcggg ccaaacaccg ccgccgcgcc
    15421 gcgtgcccct accagcgggt tatcaacgtc acatgctact tcaatatggc aattcgccag
    15481 acgcggatcg cattcatcca tctcaatgct tgccacgcgc gctaaattac cgccgttagc
    15541 caccagcacc tgaccgtcgg catcaaggaa acgcacaccg agcgcctgcg ccatgcccat
    15601 accaccgtcg accgtcgcac tgccgccaat gccgagaata atatggcgaa tgccgttatc
    15661 cagcgcatga cgaattaact cccccgtacc aaaactggag gccagcaacg gattacgctt
    15721 ttcaggcgcg accagcatca ggccacttgc tgccgccatc tcaatcaccg ccgttttccc
    15781 gtcgccggta aggccataaa aagcattcac tttttcgccc atcggcccgc agacttcaag
    15841 cgtcacgatg ttgccgcccg tcgcggcgac catcgcatcc accgtccctt cgccgccatc
    15901 cgctatcggc aaacagatat agttcgcatc gggaaagagg gtcgaaaacc cggctttaat
    15961 tgcctgacaa catttttctg cacttaagct ctctttaaaa gagtctggcg caatgacaat
    16021 cttcattatc aatccttttt caacaaatta ttcgatgcgt tcccacggaa taagattatc
    16081 cataagaaca gccagtaaaa tcccgaccag taagccgtta ctgagcagag gacgcagcgt
    16141 taatggaagg tcttgcagat acacaggcgg taatgccatt aaaaatatgc cgacaaataa
    16201 cggcaatgcg agtcgataaa tattgcgagc ggtaaacgtt atttgctggc taaacaccag
    16261 cgcggaaaag agtaaaggca gataagaaac cagcatgacc gcactactca cgggtaaagg
    16321 gatactgcaa aacagtcgtg tgagtgccgg aaccagcgcc accagaaggc aaataacgct
    16381 gccataaata aacgaacgcc gcgtgtaatc gcctgtctgg gttaataaac caatggatga
    16441 aacaaacggt gaaaatggaa ttaccgccag cggtacggtt atcagcgtca taaatccggt
    16501 cgccacaaag ctgcgacgat aacgcgtatt ccccgcgccc tgctgcggat aaaaaacatc
    16561 cgtgccccga atcgcaccgt aggtattgct gatattcacc agacctgtaa tcaccgccgt
    16621 cagaataatt cccggagaca aagcacctcc actgccgagc gggaaccact gccagtgcaa
    16681 ctcaccggag agcgagtgcg aagaaggaaa gcaaaagtac cacaacaacc agccggttat
    16741 ggtgccgacc agcaggccat aacgggcaaa acgttgcggc aggaagataa tcatccccag
    16801 taccaggcac atcaccgcca ccgagagcgc gaacggcggt aactgaattt taaaatttgg
    16861 gtcggctatg ccaaacggca gcccgagcat acctttgaaa aagatagtgg tcagctgcgc
    16921 gcccagcatc aacataaaca agaccatcac cgacggcgta aataaccgtg ccaggcgatg
    16981 gcctaatccg ctaaaaccaa tcaacatcgt cagcacgccg gagagcgcaa tccccaccgc
    17041 caggctggta gcgatatcgt tgatcggtgt cccgcgcgat gcttcgccaa gggtgatagt
    17101 aaggatggtt ccccaccact ggccacccgg cccttccata atagcgcgac gatgaccgca
    17161 gaacgcctga gcgaagcagg ccagtgcggt agcaagaaaa gcatattgcg tgagcgtaag
    17221 caggctactt tgcggcaact gaaaagcaga aagtaacgta ggaggaacca caaccgtgtt
    17281 gcaaaaaata aagaaaaacc actgaaatcc tgataacagg ctttcgcggc tgactgcaaa
    17341 attgaacata aacacacctt aaccggagaa tgcccgccgc aaggacgggc attgcagggg
    17401 ccagattact gctgatgttt aaggataaat tgacctttcg gcgcaacagg gaagccctgt
    17461 tcaatgtcgt aaatcacatc accacgtaag atggttttcg tgatacgcgc gccaatggta
    17521 cggccaacat acgggctgac tttgtggcga tattccagat cgtcattggt aagaacatag
    17581 ctgctattcg gctgaatgaa gacgaagtcg gcatcttttc ctggggcgat acggcctttt
    17641 tgctgcagac cgaaaatatc tgctgcgtta gtcgccatta atttgccgaa cattggcaga
    17701 gacattccgc gtttctgtac cgcttcatcg aacatcacgt ccatacagtt ttgcagaccg
    17761 gcaataccgc cccatgcttc catgatgttg ccggctttca tttccggagg gcatggtgag
    17821 tggtcggaaa ccaggcagtc gatttcaccg ttaaacagtt tttcccacat gcctttctgg
    17881 ttttccagat cgcggatcgg cggtgaacac ttcgccagag tgccgatttc ttcgaactga
    17941 tcggtatcca gtacaaagta atgcgggcag gattcacaag taacgtcctg accttcctga
    18001 cgtgcacgag tcacttcttc aacgccttcc gggctgctga tatggcaaac gtgcagacgg
    18061 caaccggcaa ctttcgccag atacagcacg cggcgaatcg cttccacttc ggtaaatacc
    18121 ggacgcgaag ccacatagtc atgggcagtt acgcgacctt cacgtttcgc ttcttcgccc
    18181 agtgcgtcac agatcagcgc gttttcgcag tgcaccagca ccggctgccc cagttcgccc
    18241 agcttctgcg cacctttgaa gaactgccag tcgtttacat cacggaagtc gttgtcgata
    18301 ccgcgatcgc cacaggtcgc aacgaagcat ttgaagccga caacgcccac ttcatccagc
    18361 tcatgcagac ggtcgatgtt gtaagacacc aggccaccga gttgtgccgc atcgatagtc
    18421 agcttgcctt tagcggcatc gaacttcagt tcaattgaag cgcggtcaac cgttgcaggc
    18481 agctggttga gcggcatttc gatcatggtg gtgataccac cttttgccgc tgcgcgagta
    18541 ccggtttcat aaccttccca gtggctacga cccggttcag aaatatgggt gtgcgcatca
    18601 accatgcccg gcgaaaccac cagaccagac gcatccataa cttcttttgc atcgcccaga
    18661 tcctgaccga tagcagcaat tttcccgcct ttaacggcga tatctacaac gcgagcttcg
    18721 ttttctaaaa taacggtgcc gtttttaatg attaaatcaa aagacataac aaactccttg
    18781 taaaatacag attggttttt aactcaatgt tcaatatcgg gattaattaa cctatggttt
    18841 tttgctctcc tgttttttct gctgttgtac gtttctttaa taaggcgtag gccgcaaagg
    18901 cgacgatgac gccgacaaac catgaaacac gcgataacgg ttccataaag ggaataaact
    18961 taccgccaag agataaaata acggccacca gagttactga aaacgcggtg aggttaaaac
    19021 cgttatcgta atatttataa tcgccaggtg cggtgtacag ttcatcaaga ttaatttgcc
    19081 cacgcatcac cacaaaataa tgtgccatca tgacaccaat taccggaccc agcattccgc
    19141 cgatgatatc gaggaacaga taaatactgt cctgattttc cattaatttc cacgggcaga
    19201 tcagcaggct gataatactg gcaatcagta cgccgttttt ataggtcagt tttgtcggtg
    19261 caatggcagc aatctgataa ccggccggaa taatattacc ggtggcgtta gtggaaatag
    19321 tcgtcatcag aataaccagt accgcaaaga acgaggcaaa caggctgtcc caacgctgaa
    19381 caatatccag cacgttccag gtgtcagcgc cgtagtgaat actggctccg gcaataatac
    19441 ataccccagc gaccgcaaac agaatatagg ccacaactaa acccagcgtt tgccccagcg
    19501 cttgctcacg aaacgagtgg gcgttttgcg taaagtcgga tgcgctcacc gccggtgccg
    19561 cccagaccgc aactaccgcg ttaatcacca ccaggaacaa gaatccactg ttttctgctt
    19621 tctgaatacc gctcggaatg tagtcaaaga ttggaccgat cccgaccagt gaaatcgccc
    19681 aaatcgccat gccgccgaaa acgatataga tgcacgggtt aagaatggca gtgaatttat
    19741 ttaaaacttt gccaccgcca aaaccaatac caacgttaac cagccagaag agtaagaaag
    19801 taattaagcc cggtagcgaa aggcctaaca aggtaaaatc accaccgaga gttaaaaatc
    19861 ccggccagat tttgccaatc aggatcaagc aggccagtga tccggcgtaa cattgcaggc
    19921 caaaccacat aatggcagca ataccacctc ttaataatcc gggaaacagt gcgccacgta
    19981 cgccgtaaga agcacgcagg atcatggcaa acggtacgcc gtatttactg cccgcagcac
    20041 cgtttaatac cattaccgcg gcaatgaaaa aggcgctgag gataattgcc agcataatac
    20101 taaaggtaga caagccgaga ataaaaaagc cgccgaccat cacataattg ggaacgttat
    20161 gaaccgaacc catccataag gtaaaatagt taaatgtttt ccaggtccgc tggctttgcg
    20221 ttttcggcaa taaatcttcg ctatagccgc gttgctggaa tagctttctc tgatgttcca
    20281 taaatccctc ttaatttcac gtatcgaata acgttgtttt aatctagcta tgaggaacaa
    20341 ataaaataaa aaatattatt tttaatactg aagcaacatc tttggctgct ttctttttta
    20401 atattgctgg ccgattcatt ttctgatatc ggccattttt attgcgggta tcaggccagt
    20461 ttatggttag ccattaattc cagcgcctgc actaacgcag agtgatccaa ctggctgcca
    20521 ccgtttgccg cacaggtatt aaataactcc tggcaggtcg cagtgtttgg caggttcagc
    20581 gcaagtgctt tcgcactttg cagtgccagg ttgagatctt tctggtgcag agcgattttg
    20641 aagcccggat taaaggtgcg tttaatcata cgctcgccat gaacttccag aatacgtgag
    20701 gaagcaaagc cgcccatcag cgcctggcgc acacgtaccg ggtccgcacc ggcttttgaa
    20761 gcaaatagca gggcttcaga aaccgcttca atattgagcg ccacgataat ctgatttgcc
    20821 actttgcagg tttgaccatc gccgttaccg cccacgaggg tgatattttt accgagcagt
    20881 tcaaacagcg gtttaacacg ttcaaatacc gcttcatcac cgccaaccat aatcgacaac
    20941 gtcccttcac gcgcaccgat ttcaccgccg gagactggcg catcgagata atcgccgccc
    21001 agttcattca cctgacgagc gaaacgctta gtttcaatcg gggaaatgga gctcatatca
    21061 acaatggttt tgcccttcag cgaagttttt gcacagccat tttcaccaaa caatacttct
    21121 tcaacctgcg gcgtatctgg caccataata aagataatgt cagaagattc agttacctgg
    21181 cgcgcggttt caacatttac tgcgcccagc gccagtaatt cttccgcaac cggaccaatg
    21241 gtcgtcacat gcagttgatg gcctgcgcgt gccaggttaa ctgccatcgg tgtgcccata
    21301 atacccaggc caataaatcc cagtttcata attaacctct tgtgaatatc gctgtttctg
    21361 aattcagaca acacgatccc ctgccggcat agcggcaaaa aatgcattgt ctgaatcgtt
    21421 tttcgtttta gcggtatgga ttaatccatg ataatcccgc ttccgtggtg gtcaggggtt
    21481 tatattcgca ccctacccaa ccgtcataat cagacttttc aattaaatcg aataaaaagt
    21541 cgtaatttat ttccccggtg ccaggttcgc cacggcgggg gttatcggca atctgcaaat
    21601 gaccgatttt atttgcccat gtagtcatgg tttgggctaa ttccccttcc atgcgttgca
    21661 tgtggtaaat atcgtactgg atcttgatat tgttactgcc aatatcggca atcagcgcca
    21721 gtgcttgttg tgttccggtc aaatggaagc ccggcatatc aaagtgatta agtggctcaa
    21781 tcagcagcaa aataccctct tttgccagca tatttgccgc atagcgcagg ttctctacca
    21841 gagtggtatg tacctgctct gctgatacgc ccacaggtgt tttaccaacc agacagttaa
    21901 tttttttatt acccagtgca cgggcataac gaatcgcaac ggctacacca tcacgaaatt
    21961 cctcttcccg gccaggaata caggctattc cgcgttcacc cgccgcccag tcacccgcag
    22021 gtaaattatg cagcgtatgc tccaggttat tactgctgag cttgtgctgc aaaacttcta
    22081 tgtcgtagtc ataggggaac ataaattcta cagcgcgaaa tccgcactgc gccgctttat
    22141 caaagcgctc aagaaaatca tattcctgga ataacatcga caagttagca gagaaacgta
    22201 acattaatta cctcccttac ttatattgca taaaacaggt ttcggttggc gcgtccgctg
    22261 cgttatcggc gatatcttca aattccatca cattatcgag ttcgttaccc atcgaaatat
    22321 tggttacgcg ctcgagaatg atttccacca ccacaggtac acggtattga ttcattaatg
    22381 ctttcgcctg ttcaaatgcc ggagcaatat cttccggttt gaaaacacga atcgctttac
    22441 aacctaaacc ttccgctact tttacgtggt caacaccgta gccattcact tcactggagt
    22501 tgatattctc gaaagcgagt tgcacgcagt agtccatatc aaacgcgcgc tgcgactggc
    22561 gaatcagccc cagataagcg ttattgacca gtacatggat gtacggaatg ttgaactgtg
    22621 cgccaacggc caattcttca atcaggaact ggaagtcgaa gtcaccggaa atcgccacca
    22681 ctttgcgctc tggatcagcg gcacaaaccc ccagcgccgc cggaatggtc cagcccagcg
    22741 ggcctgcctg cccacagttg atccagtggc ggtctttaaa gacatgcagc atttgcgcag
    22801 cggcaatttg cgacagacca atggtggtga catagcaaac atcgcgacca aaggctttgt
    22861 tcatctcttc atacacgcgc tgcggtttca ccggcacgtt gtcgaaatgg gttttgcgca
    22921 gcaaagtgcg tttgcgctgc tggcagtcgg cgacccattc tttacgacac ggcagacgac
    22981 ccgctttttg catctcctgc gccacttcaa ccagcagtgt cagcgccgct ttagcatcag
    23041 agacaatgcc gagatccgga cacagcacgc gaccaatttg cgtcggctca atatcgatat
    23101 gaacgatttt gcgcccttcg gtgtatttct ctaccgaacc ggtatgacgg ttagcaaaac
    23161 ggttaccgat accaaacacc atgtcggacg ccagcaacgt tgcgttaccg taacgatgcg
    23221 cggtttgcag acccaccatc ccggccatca gttcatgatc gtccgggata cagccccagc
    23281 ccatcagcgt tgggatcacc ggaacgctgg tcagttcagc aaactgttgt aacagtgcag
    23341 ctgcgtcagc attaattacc ccgcccccgg caacaatcac cggacgttcg gcctggatta
    23401 acatttctac agctttttcg atctgcatac ggctggcagc aggtttgtag accggcagcg
    23461 gttcgtacat atcaggatca aactcgattt ccgcaacctg aacgtcgaac ggtaaatcca
    23521 ccagtaccgg acccggacga ccagaacgca tcaggtgaaa tgcctgttgc aacacacgag
    23581 gcaccagcgc cgcttcacgt actgtaaccg ccattttact taccggcttg gcgatagctt
    23641 cgatatcaac agcctggaaa tcttctttat gcagacgggc gcgcggagcc tggccagtaa
    23701 tacacagaat aggaatggaa tcagcagaag cagaatagag cgcggtgatc atgtccgtgc
    23761 ccgcaggacc ggaagtcccc agacatacgc cgatattccc tgccgttgcg cgggtataac
    23821 cttccgccat gtgcgaagca ccttccacat gacgcgccag aatgtgacga ataccgccgt
    23881 gcttacgcat cgctgagtag aacggattga ttgcagctcc cggaacgccg aaggcggtgg
    23941 tgataccttc tttctccagc acatacattg ccgcgtcaac ggctctcatt tttgccattt
    24001 tattcctacc tctattaatt ggaaaaattt tccaactttc aaattttgtc aaaaaaaccc
    24061 gctccgcaga gcgggatgcg acagacacta tggatgtgct ttcagtccca acgccgtgct
    24121 gatatcgcgg gcggtgtctc tgaccagctc accctgactg acaaaacgat cttctgtcag
    24181 tcttgatgaa ggcccggaga tggagatagc ggcaacaacg ctaccgacat catcgtaaat
    24241 tgctgaagct atgcaattca gacctacaac atgctcttct ttatctacgg tatagcccag
    24301 ttcacgcgct tgttccaggt ccttcagcaa ggtgggcata tccacaagcg tagttggcgt
    24361 aaactgctgc aaaccggttt gcagaatgat gctcatcaac tcctcttccg ccagcggata
    24421 aagcagcgct ttgcccgcac cggaagcatg cagcggcaga cgactgccca gtggcgcaca
    24481 catcctgacc attgatttac actctaactg accaattaat actgcttcat tgccgttacg
    24541 gatcgcgaca ttgaccgttt cgccggaaag taacatcagg cggcgcataa acggcccggc
    24601 gacggagagg acatcgcggt tatggatgta cgccgcaccg acgttaaaga cacctaatcc
    24661 tatatgccac cagcctaatt gactgtcctg atagacaaaa tccgctgcct gtaaaacctt
    24721 cagcaagcga aaggtcgtgg agagcggcaa atccagattg agagaaatat cgctaaccga
    24781 cgaacttccc ccactttttt ccagatattg cagaatcgca attccccgct ctaacgcctg
    24841 tgcgcccttc tgtgccacag gctccgcctg tcctggcctg ccgcgccgtc taacttccgt
    24901 catacctacg ttaactccga cctacaagtg agcataaaca atgaacaatt agtttatctt
    24961 atgcaagtcg gttgcaggcg tcacgcaaaa cagagttcct gttcaggaat actttcaaca
    25021 tcacagttgt cactgccgcc gcgatcgatg gtcagaaaat cggtgacgcg ctgccaggcg
    25081 aaaagtgggt gatgccagac gttacgatgg taattcactc cctgttcgcc gttggtgata
    25141 aacgcccgca gcgttgacag gtctggtttg tcgtcgccta acgccacgac caccacaaac
    25201 acttcacctt tcatcgggat aaaggcctga gtgcccagcg gatgacgttc gagttcgtaa
    25261 atggtcagcg gcagattcgc cggttgcgcg cggttaatgc tgataagcgt acggtcttgc
    25321 tcaagaatct caaccagcgc caaatcgtgg taacgctcca ccaggccatt gttaatatgg
    25381 aaaaaatccc gttgctgagt ttcgattacg tcgccataag cactaaaggc ttcctgactt
    25441 aacggtaata cctgaagttt catctttttg tccttgtaac ataattccgt tcaaccatcg
    25501 ccgaccggtc agcttggttt caataacagg caatctaaaa ccttaagaga acaacatcaa
    25561 tacaaaagtt ggaatatttt tccaaaacta gatttagtcc acaaattgaa cagggttatc
    25621 atgtaaaaat actgataaat cagaggataa gaaaatcacg caacttgcgg atttaaaagg
    25681 ggatacctga agatgttcga tccagaaacc ttgcggactt tcattgcggt tgctgaaaca
    25741 ggaagttttt caaaagcggc agaacgatta tgtaaaacca cggcgacgat cagttatcgc
    25801 attaaacttc tggaagagaa taccggagta gcgctgtttt tccgcacgac tcgcagcgtg
    25861 acgttgacag cggctggcga gcatctactt tcccaggcca gagactggct gagctggctg
    25921 gaaagtatgc caagcgagct gcaacaggtg aatgatggcg tggaacgcca ggtgaatatt
    25981 gtcatcaaca acctgctcta ccacccccag gccgtcgccc agttgctggc gtggctgaat
    26041 gaacgttacc cctttaccca gtttcacatc tcccgacaaa tctatatggg cgtctgggat
    26101 tcgctattat acgaaggttt ttcactggct atcggcgtca cgggaaccga ggcgctggca
    26161 aataccttta gtcttgatcc cttaggatcg gtgcaatggc gctttgtcat ggcggcggat
    26221 catccgctgg cgaacgttga agagccgcta acagaagcgc agttgcggcg ctttccggcg
    26281 gtcaatattg aagacagcgc ccgcacctta accaaacgcg tcgcctggcg attgcctggg
    26341 caaaaagaga ttattgttcc tgatatggaa acgaaaatcg ccgcccatct tgcgggcgtt
    26401 ggcattggtt ttttgccaaa atcgctttgc cagtcaatga tcgataatca acaactagtt
    26461 agccgggtaa tcccaacgat gcgccctcct tcgccattga gtctggcatg gcgcaaattt
    26521 ggcagcggca aagcggtaga agatattgtg accttgttta cccagcgcag gccggaaatc
    26581 agcggatttt tagaaatttt cggcaaccca cgcagttaaa aatcaagttt ccttgcgcgt
    26641 tggcgcgtaa aatagcgcgc aagctgattt gaggttcact tttaactatg caagagagac
    26701 acacggaaca ggactatcgt gccctgctga ttgctgatac gcccattatt gatgttcgcg
    26761 cccctatcga gtttgagcaa ggcgcaatgc ccgccgctat caatctgccg ttaatgaata
    26821 acgatgaacg cgccgccgtt ggcacctgct ataaacagca aggctcagac gcagcgctgg
    26881 cgctggggca taaactggtg gcgggtgaaa ttcgtcagca gcgcatggac gcctggcggg
    26941 cagcgtgcct gcaaaatccg caaggtattc tctgctgcgc ccgtggcggt cagcgctcac
    27001 atattgtgca aagctggttg tatgcagcgg ggattgatta tccgctggtg gaaggcggtt
    27061 ataaggcact gcgccagacc gcgattcagg cgactattga actggcacaa aaaccgatag
    27121 tgctgattgg cggttgtact ggcagcggta aaacgctgtt agtgcagcaa cagccgaacg
    27181 gtgttgatct ggaagggctg gcgcgccatc gcggttcggc gtttggtcgc acgttacaac
    27241 cacaacttag ccaggcgagt tttgaaaacc tgctggctgc cgaaatgctg aaaaccgacg
    27301 cccgtcagga tttgcgcctg tgggtgctgg aagacgaaag ccggatgatc ggttcgaatc
    27361 acctgccgga atgcctgcgc gagcgaatga ctcaggcggc gattgcggtg gtagaagatc
    27421 cgtttgagat ccgtcttgag cgcctgaacg aagagtattt cttgcgtatg catcatgatt
    27481 ttacccacgc ttatggcgac gaacagggct ggcaggaata ttgcgaatac ctgcatcacg
    27541 gactttcggc gattaagcgt cggctggggt tacagcgcta taacgaactc gctgcccagc
    27601 tggatacggc gctgacaacg caactcacca ccggcagcac cgacggtcat ctggcctggc
    27661 tggtgccgtt acttaaagaa tattacgacc caatgtatcg ctatcagctt gagaaaaaag
    27721 cggaaaaagt tgtctttcgc ggtgagtggg cggaagtggc ggaatgggtt aaggcgcggt
    27781 aatgagggac gtatttgtat tagtcgcgac ttgatctgac acatggcctt gaaaggttga
    27841 gagttaccgg ttttgatatg agtgtcgaat ccttatccaa aacaagaggt aactctcatg
    27901 cttcatactg ccaatcccgt catcaaacac aaagccggtt tgctcaatct ggctgaagaa
    27961 ctcagcaacg tatcaaaagc ctgtaaaatc atgggcgttt cgcgtgatac gttttatcgt
    28021 taccgcgaac tggtcgctga aggcggtgtg gatgcgcaga ttaatcgtag tcgccgcgct
    28081 cctaacctta agaaccgtac cgatgaggca actgaacagg ctgttgttga ttacgccgtc
    28141 gctttcccga cacatggtca gcaccgggcc agcaacgagc tgcgtaaaca gggggtttta
    28201 tctccgacag tggtgtccgt tctgtctggt tactccata
//
LOCUS       DAPHDX010000054        27987 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPHDX010000054 DAPHDX010000000
VERSION     DAPHDX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.054"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..159
                     /locus_tag="RH058_002251"
     CDS             <1..159
                     /locus_tag="RH058_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3137838.1"
                     /translation="IFQWLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQ
                     PLILPDRR"
     gene            complement(268..3783)
                     /locus_tag="RH058_002252"
     CDS             complement(268..3783)
                     /locus_tag="RH058_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3137839.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSVGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSINFDDGIDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGTGAMAGRWLPELKSFGAYFPSSTRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKDCSSPEKVIELLHGQLAAKSGQLIRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTQLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQYQIQLFLEDKLGALIAGNQWSEPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIILKEQQAVQEWQRLALDLGFEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMSLYE
                     HRRCFDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGEQASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQALGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EIQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDNFLNQTF
                     ENTDKHITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4235..5497)
                     /locus_tag="RH058_002253"
     CDS             complement(4235..5497)
                     /locus_tag="RH058_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV3137840.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRNEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRISS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5695..5770)
                     /locus_tag="RH058_002254"
     tRNA            complement(5695..5770)
                     /locus_tag="RH058_002254"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5735..5737),aa:Phe,seq:gaa)
     gene            complement(5876..6583)
                     /locus_tag="RH058_002255"
     CDS             complement(5876..6583)
                     /locus_tag="RH058_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV3137841.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTEAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSII
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6981..9116
                     /gene="speC"
                     /locus_tag="RH058_002256"
     CDS             6981..9116
                     /gene="speC"
                     /locus_tag="RH058_002256"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3137842.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9166..10422)
                     /gene="nupG"
                     /locus_tag="RH058_002257"
     CDS             complement(9166..10422)
                     /gene="nupG"
                     /locus_tag="RH058_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV3137843.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10624..11703)
                     /gene="mltC"
                     /locus_tag="RH058_002258"
     CDS             complement(10624..11703)
                     /gene="mltC"
                     /locus_tag="RH058_002258"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV3137844.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11768..12043)
                     /locus_tag="RH058_002259"
     CDS             complement(11768..12043)
                     /locus_tag="RH058_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV3137845.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(12071..13123)
                     /gene="mutY"
                     /locus_tag="RH058_002260"
     CDS             complement(12071..13123)
                     /gene="mutY"
                     /locus_tag="RH058_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV3137846.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13284..14003
                     /gene="trmB"
                     /locus_tag="RH058_002261"
     CDS             13284..14003
                     /gene="trmB"
                     /locus_tag="RH058_002261"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV3137847.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAYEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            14003..14329
                     /locus_tag="RH058_002262"
     CDS             14003..14329
                     /locus_tag="RH058_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV3137848.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            14513..15232
                     /locus_tag="RH058_002263"
     CDS             14513..15232
                     /locus_tag="RH058_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV3137849.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15408..16454
                     /gene="ansB"
                     /locus_tag="RH058_002264"
     CDS             15408..16454
                     /gene="ansB"
                     /locus_tag="RH058_002264"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV3137850.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16571..17578
                     /locus_tag="RH058_002265"
     CDS             16571..17578
                     /locus_tag="RH058_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV3137851.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(17733..18869)
                     /gene="hemW"
                     /locus_tag="RH058_002266"
     CDS             complement(17733..18869)
                     /gene="hemW"
                     /locus_tag="RH058_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV3137852.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(18862..19455)
                     /locus_tag="RH058_002267"
     CDS             complement(18862..19455)
                     /locus_tag="RH058_002267"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV3137853.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(19463..19753)
                     /locus_tag="RH058_002268"
     CDS             complement(19463..19753)
                     /locus_tag="RH058_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV3137854.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(19750..20316)
                     /gene="yggT"
                     /locus_tag="RH058_002269"
     CDS             complement(19750..20316)
                     /gene="yggT"
                     /locus_tag="RH058_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV3137855.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(20334..21038)
                     /gene="yggS"
                     /locus_tag="RH058_002270"
     CDS             complement(20334..21038)
                     /gene="yggS"
                     /locus_tag="RH058_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV3137856.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            21056..22036
                     /locus_tag="RH058_002271"
     CDS             21056..22036
                     /locus_tag="RH058_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV3137857.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(22212..22628)
                     /gene="ruvX"
                     /locus_tag="RH058_002272"
     CDS             complement(22212..22628)
                     /gene="ruvX"
                     /locus_tag="RH058_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV3137858.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(22628..23191)
                     /locus_tag="RH058_002273"
     CDS             complement(22628..23191)
                     /locus_tag="RH058_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV3137859.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(23300..24250)
                     /gene="gshB"
                     /locus_tag="RH058_002274"
     CDS             complement(23300..24250)
                     /gene="gshB"
                     /locus_tag="RH058_002274"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV3137860.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(24263..24994)
                     /gene="rsmE"
                     /locus_tag="RH058_002275"
     CDS             complement(24263..24994)
                     /gene="rsmE"
                     /locus_tag="RH058_002275"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV3137861.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(25074..25781)
                     /gene="endA"
                     /locus_tag="RH058_002276"
     CDS             complement(25074..25781)
                     /gene="endA"
                     /locus_tag="RH058_002276"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV3137862.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(25876..26373)
                     /locus_tag="RH058_002277"
     CDS             complement(25876..26373)
                     /locus_tag="RH058_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV3137863.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(26450..27844)
                     /gene="galP"
                     /locus_tag="RH058_002278"
     CDS             complement(26450..27844)
                     /gene="galP"
                     /locus_tag="RH058_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV3137864.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6938 a         7054 c         7135 g         6860 t
ORIGIN      
        1 atttttcagt ggctgaccgg cattcgtact gaaaatatac caccattcaa tccgctgaca
       61 gacgaagata atgaacccgt tatggttttt aacggagaaa ggaatgtacc ggcagacagt
      121 ctgaaaccgc agcctctgat tcttccggac agaagataat catgtcggta tgaatgttat
      181 aattaaggac tgtgcaataa tagccattcc attcagccat cttacgctcg aaaacaatca
      241 ataagatggc tactcaccat tcctgctcta aacctgacgc ctctcaagac tattataaat
      301 agcctcaatc tcacccttca ggttatcacc gcaataaacc attgaggtat cgtatcgttc
      361 atatcgcgct tcccgtgtcg cactaaacca gttgaacgat cccacgcaca gcaaaccatc
      421 atcaccaata acaattttgc tatgaacacg gtttaccagc tttgtagcaa tacccagcgc
      481 attcagtttc tccagcgccg ctttaaagtt ctgctgcttc tcttttcgct tctcaaaatc
      541 attatgttca gtgttgtagc ttctgtcagt gactatcgtg acgttaattc cacgtgaaca
      601 cgccgcaatc atggaatcaa gaaaacccgt ttgctccagc ttttgccagg tcagccatgg
      661 agaaactatc gtgatgtgtt tatcggtatt ttcaaacgtc tgattcagga agttatcatg
      721 ctgctccaca ccatgaagtg tgtagatttt ggtcccggaa gtttttaaat ccttacgctc
      781 tttataatca aaagagagcg cattcttctc ggactcaaag agatattttg ccagcaatcc
      841 tcgcggagat gagggaggct ggatctcaaa cagatccata tcgccgaaga ccaggaagct
      901 atccttcgca cgggagactg caacattcag catgctgtta tcgctatcaa taaacccgcc
      961 gtcttcatgt tttgaataga ctggcgagaa tataacaatc gctctttccg ctccctgaag
     1021 agagtgcacg gtgcccactg tgagcgactt ttcattcgtg cctgtactga taccttgttt
     1081 acccagcgcc tgttttatag tggatacctg cgcgctaaaa ggcgtcacga taccgacaac
     1141 ttcatgaagc gatttgccat aatgcgcttc aatattttgc tggttctctg ccaaccaggc
     1201 cgctatcgtt tcagcctcaa gcaaattata tcgacttcca ctacttgcct gctctccttt
     1261 accatcaata tggagataac ccattgcggg cattaaattg ctctcttcac gccctctttt
     1321 aggcaacaac ttaccgtgat agcagagcgt attacagtat ccaataatat tatcgaagca
     1381 ccggcggtgt tcatataggg acataccacg agccagttcg ggatcatatt gatagcgcga
     1441 agcaaactgc gctattttca taacgctgcc agatgcggca cttttaccaa ggtctgcgat
     1501 tgccgtatat ttctcggtaa tctcttcttg cgtactgcca gacagaattt tttccgccag
     1561 catgttaccg acatcaatcg cgggagcaat actccatatt ggcgggatct gctccgtatc
     1621 gccaatcact aatgccttct tagctaatgc aaacgaggca gcagccactt caggaagcac
     1681 ctgcccggct tcatcgacaa tgagtaaatc ggcaaaatca tacaaataac ttttctcgaa
     1741 tttacgctgt cctttgtgct cacttatctg catattgccg ggcagcatat agcatgtcat
     1801 caccacacat ggagtgagtt tcattcgacg ttgccaacgg gcggttaccc ctttagcgcc
     1861 ttttttcttc ttctcttcct gcagatcatc aatgctggcc atatccatca gccaacgacc
     1921 ttcccagtag tgagtcgtca gtaaaaatgc agggaagcga atctgcgtat cagccagttc
     1981 atcggcctgt gagaagctca gttcctcgtc gccctcaaac cctaagtcaa gagccagtct
     2041 ctgccactcc tgaaccgcct gctgttcttt aagaatgatt tcatgggcgg agtcaatctg
     2101 ctgccggtat gttgtttgct cgcgctcagc ggaattgagc agcccatcaa tattacgttc
     2161 gatagtttca ggctcagacc actgatttcc tgcaatcagc gcgcctaatt tatcttcgag
     2221 aaacaactgt atttggtact gtcgcttact acgaaccgcc gggagccagg aaaataatga
     2281 atagatcagt gattcaccgg cgcgatattt tttccattcc gttttagcac tcttcagttg
     2341 agtgactttt tgttcttgtc cggaaagtaa tttatttaaa ttatcgagat attgctcaat
     2401 gtcgttagca ataagctcac gcgcagcccg tacctggctt aacgtttgcc atgttgcgtt
     2461 cagtcttatc agttgcccgg attttgctgc caactgacca tgcaggagtt caatgacctt
     2521 ttcaggggat gaacaatctt tttcaggaaa ggctgcctta gccttctcca gataaaacag
     2581 cagtgcatcc tctacatact cttttgactc aacctggttg aagaaatctt cagtttgata
     2641 ttttttggct gcctcagctt tacgagtgct tgagggaaaa taagcgccga agcttttcag
     2701 ctcaggtaac catcgtccgg ccattgcacc agtgccctgt gaaaaatctt tcccgaacgc
     2761 ttcgataatg ttcgttacag cctggttgtt cgttgaagtc gcgataataa ccggaggctc
     2821 agatttttcg agagccgctc tggcccactg cgtggcgatg atagaaagca ccagcgtggt
     2881 ttttccggtt cccggaggtc cattaacagc aaggatatcg ccatgtcttg catccagaaa
     2941 atggcttaag gcatcgcgct gagcctttgc cagcggaaac ttatctccgg agtgtccaag
     3001 cctgtcgctg aattttgcgc ctggggccag caaagactct gcagcatgaa cctctcgcga
     3061 ggcgaagcga ttgaagagcg gcacatcctt cttgcaaaca agcaggtgat cataaagaga
     3121 aaggatatgg aaactggcac cgccagattg cgccgtttta acaatataac cgtgctcagc
     3181 gagttcatat tgctcaggat ttttaatcca gtcgccggca acgttcttca gcagcctctc
     3241 tgaatcatcc agatattgac gccactcctg ctgcaaggct gcatatcgtg cttcccgttc
     3301 ttcatccgtt tcggcagtct tatcaatgcc gtcatcaaag ttgatagaga atgaggtatg
     3361 gattgtcttg tatttgtcat actgcccaat ctcaccaatc gaaaatgctc cttttggcaa
     3421 aggttcaagc aggtctctgg gaatggaggt cgctggcgtc ggatataaaa aaccctcacg
     3481 gcttagcaag gctggcgtca ctatcggggt aacaatatca ggcgcgccta cggaacggtc
     3541 cttaccatgc tgcagtaacc ggaaataaac ttttgggcgc aagatgacat cgaccgtttc
     3601 gacatcgtct ttttctccct caaaaaattt actgacaatc gcttcgtcaa gacgtcccgc
     3661 cgctatccca tgccagtgag tgaaattttg ggcgtctttc cgtttaaaac tgccctttcc
     3721 tgactcagca tccgcaagcg agttgcgcca gtatgaggca aaccctaaag cattttcatc
     3781 cattaccagt cctgtgactc aatgaaatcc attgatttat cgaatataaa atcaccgtat
     3841 ggacagctat tgtccatatt gaaaaagttg gatgcaatgt tttgtttata attcgatatc
     3901 aaatgaactg gttgtacgag gttcagaaca caagctcatt aaacggtaag tcaggactat
     3961 tgatttcata acagtgaata cattttttac cgtttataat aactgcctgt gttggacggg
     4021 gataccagag cagccgaaaa caccgggggt tattacctgc tctcatggaa accatatatt
     4081 cctggtgcta atcacagtca taaatagcct gaagaacgtg acgaagggac tttaatggac
     4141 gccggagtaa cttgtcagaa acatcaatct tcgtcatcat ttcaaagaaa aataatcagt
     4201 taaagcagtc cgggctgatt gcccggactg cttactattt taatggattg ttaatctttg
     4261 catattcaaa cggactgata cccttctccc ggttcgcatc cagaaaatcg gcccaccatt
     4321 gcaacatcag tcgccgttct tccagatgtt ctgctttatg gatatacgca gcacgtacac
     4381 cattacgctc ctgatggctc atctgacgtt ccacagcatc gcgggaccac aaacctgatt
     4441 caatcaatgc actgcacgcc atcgcccgga atccatggcc acagacttcc tgggttgtgt
     4501 cataccccat caccctcagg gccttattta cagtattttc actgattggt ttacgcgggt
     4561 catgtgcacc cgtaaatatc agaccatttt caccagccca tgtctgaagt tctgtcagta
     4621 tggctacagc ctgtttgctg agtggcacaa gatgtggtgt tcgcatcttg gagccccggt
     4681 gtgagaattt tacgccgggc agaggttcgc gttcggctgg aatagtccac atggcattgt
     4741 caatatcaat ttcagaccac cgggcgtatc tgagttcact ggaacgaata aaaaccagta
     4801 aattcagcat aacggcaagc tgggtgacag gttgcccctt gtaactgcta atacgcgaca
     4861 aaagttcagg caggcgagaa agatcaagag cggggcgatg ctggcgtttt accgtggtca
     4921 atgcgccagc catatccaga gcaggattat atctgatgat gccagactgt acggcataac
     4981 gcattacagc agatattctt tgctgcagac gactggcgat ttcataaatt tgtttagctt
     5041 ctgcggcacg aacggggata agcaggtccg gggtactgag tgttgtgata tcccggttgc
     5101 cgataaaggg gaaaacgtgt gtctcgaggc ttttgagtac tttatcgctg tgtgattgtg
     5161 cccattgttt attactggca tgccagcgtc gtgcaatatg ttcaaaagag agttgtaaat
     5221 cagggactac ttttttagct cttttttgtt cgcaagggtc aatcccctcc gaaatcagtt
     5281 ttcgggcctc attccgtttt gtacgagctt cggagagaga tacttctgga tacacaccaa
     5341 gtgccagagt cttctcttta cccagaatac gataacggag acgccagtaa cgggaaccat
     5401 taggatgtac cagcaggaac atgccagccc cgtcagtgag tttataagcc ttgtcagtgg
     5461 gctttgcagc ccggattttt gcgtcagtca gtgccatagt tcagccttct gaatgcgtta
     5521 aagagggtat aaattctgtc gaactggggt atacccgcaa ttatacccac acaatggggt
     5581 tgatgtgggg ggaatctggt tgagttcggt agaattgatc tggaggcaga gcgcttaaat
     5641 tgtggcgtcc tgaaacgaaa aacggacctc cgtggaggtc cgttgatatg aatttggtgc
     5701 ccggactcgg aatcgaacca aggacacggg gattttcaat cccctgctct accgactgag
     5761 ctatccgggc aacggggcgc attaaacctg attcgcctcg tctcgtcaat caaatttctt
     5821 caatttactg cagactgcac aatctatcat cactttgccg ctattgcacg cattgtcagg
     5881 caaaccaggc ggtccaggcg gcggaaagcg gcattgccag aaggagtgct ggaagcatgt
     5941 tgaccaccgg gaacatttta atgccacaga tgcgtaatcc ggttgccagc agcaataaac
     6001 cgcctacggc gctgaagtct gccatcatcg acggtgtggt cagcggtaat atcagcgcgg
     6061 cagcccacgc cagcgttaac tggatgatca gtaatgggat actaattatc gacaccgcaa
     6121 tacccagcga gcaggcgaag atcatcgccg taaagaaatc aagaaatgac ttggcgatta
     6181 aaatactcgg atcgccggtc atcccttcgt tcatcgcccc gaagatcccg gtgccgctgg
     6241 cgcaaaacag gacaataatc gcgacataat tctgaataaa agattcatgc gctggcttct
     6301 tacgtgagtg acgaaacaga ttttgtgctt cggtgactgc tgtattgaca cctttctcca
     6361 gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc taaaaccatc gctggaaggt
     6421 tggcacattt caccaccagt aaaataccaa tccccagcga tgccagacca aaaatagatg
     6481 tcatggagac gcggatacgt tccggtaagc gttggctgag cagtgcgccg agaacgccgc
     6541 ccagtaatac tgcactagcg ttgataaaag ggccgatgac cacaagagtt cctgttaact
     6601 gtattgtttg attttcttat tatgaccgtt tggtgccgtc gatggcttgt ttgttcgcaa
     6661 agtcctggct tgcacgcttt agcgaaaggt gccatgattg cgcgaatttt ctcctctctg
     6721 tacggagttt gcccgatgca cgccacctcc ttacattctc tcgcttatcg ccgtttcgcg
     6781 cgaaacgttt ccctttctat gttactgctc atgcggtgaa gttaacgcac gctcactgca
     6841 ggacaacagt aaaatcagag cgtttctgct tttactgatg tctggcggtc ggagctggtg
     6901 accagtttga cccatatctc atggggcagg gttttccacc ttgtccggta ttcttacttc
     6961 cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc cgccagtagt gatctggtat
     7021 cccgactttc ttctcatcgt cgcgtggtgg cgttgggaga tactgatttt acggacgtcg
     7081 cggcagtcgt cattaccgct gcggatagtc gcagtggcat tcttgcgttg cttaagcgca
     7141 ccggttttca tctaccggtg tttttgtatt ccgaacatgc tgttgaatta cctgcgggcg
     7201 ttacggcggt aatcaacggc aacgagcagc agtggctgga gctggaatcc gcagcctgtc
     7261 agtatgaaga gaatttgctg ccaccgtttt atgacacgct gacgcagtac gttgagatgg
     7321 gcaacagcac ctttgcttgc cctggacatc aacatggtgc gttttttaaa aagcatcctg
     7381 ccggacgcca tttttacgat ttctttggtg agaacgtctt tcgcgccgat atgtgtaacg
     7441 ctgacgtaaa attgggcgat ctgcttattc atgaaggatc ggcgaaagat gcgcagaaat
     7501 tcgcagccaa agtctttcat gccgataaaa cctattttgt gctgaacggc acatcggcag
     7561 cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga tctggtgctc ttcgaccgta
     7621 acaaccataa gtcgaatcat cacggcgcgc tgattcaggc gggggcgacg ccggtctatc
     7681 tggaagcttc acgcaacccg tttggtttca ttggcggtat tgatgcgcac tgttttaatg
     7741 aagagtatct gcgccagcaa attcgcgacg ttgcgccaga aaaagccgac ctgccgcgcc
     7801 cgtttcgcct ggcgattatc cagctgggaa cctatgacgg cactgtctac aacgcccgtc
     7861 aggtgatcga taccgttggg catctgtgtg attacattct gtttgattcc gcgtgggtcg
     7921 gttacgaaca atttatcccg atgatggcgg atagctcgcc gctgctgtta gaacttaacg
     7981 aaaacgatcc ggggattttt gtgacccagt cggtgcacaa acagcaggct ggattctcac
     8041 agacgtcgca gatccataaa aaagataacc atatccgcgg acaggcgcgt ttttgcccgc
     8101 ataagcggtt gaataacgcc tttatgctcc atgcttctac cagccctttc tatccgctgt
     8161 ttgctgcact ggatgttaac gccaaaattc atgaagggga gagtgggcgt cggctgtggg
     8221 ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat tcttgcgcgc tgtaagctgt
     8281 tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg gcaggattat ccgacatctg
     8341 tgttagccag cgaccgccgt tttttcagtt ttgagccggg ggcgaagtgg cacggctttg
     8401 aaggatatgc cgcggatcag tattttgttg atccgtgcaa gctgttactc actacaccag
     8461 gtatcaatgc cgaaactggc gaatatagcg actttggcgt tccggcgacg attctggcgc
     8521 actatctgcg tgagaacggc attgtgccgg agaagtgcga tctcaactcc attctgttct
     8581 tattaactcc ggcggaaagc cacgagaagc tggcacaact ggtggcgatg ctggcgcaat
     8641 ttgaacagca tattgaggat gactcgccgc tggctgaggt gttgccgagc gtttataaca
     8701 aatatccggt gcgctatcgc gactacaccc tgcgccagtt gtgtcaggag atgcacgatc
     8761 tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat gttccgccag cagagtttcc
     8821 cgtcagtggt gatgaacccc caggatgcgc atagcgctta tattcgcggt gaagtggagt
     8881 tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga aggggcgttg ccttatccac
     8941 ctggcgtgct ttgcgtggta cccggggaag tctggggtgg ggcggttcaa cgttatttcc
     9001 ttgcactgga agaaggggtg aatttgttgc cgggattttc gccggagctg caaggtgttt
     9061 atagcgaaac cgatgcggat ggcatgaaac ggttgtacgg ttatgtgttg aagtaagaat
     9121 aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg cgtaattagt ggctaaccgt
     9181 ctgtgtgcct gtcgggacac gaacgtgttt atatttgaac atcgccatga acgcgaaggc
     9241 cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc tgccagtcgg taatgccgtt
     9301 ttgggtgtac atctcaacaa ctttaccgct cacgatgccg ccgaggatac agccgaagcc
     9361 gttagtcatc atcaggaaca tcccttgtgc actggcgcga attgccgggc taacttcttt
     9421 ttcgacaaac accgaaccag agatgttgaa gaagtcgaat gcgcaaccgt aaacgatcat
     9481 cgacagtacc agcagtacag taccgaacgg agtcgggtcg ccgtaagcaa acagcgcaaa
     9541 acgcaggatc cacgccacaa tactgatcat cattacgttc ttaataccgt agcggcttaa
     9601 gaagaacggg atggtcagaa tgaacagggt ttcagagatc tgcgaaatcg acatgatgat
     9661 tgacgcatgc tgcacaataa agctgctggc aaacatcgga tctttgtcga agctgtgcag
     9721 gaaggtatta ccgaacatgt tggtaatctg cagttccgcg cccagcagca ttgagaagat
     9781 aaagaagatt gccatacgct tgtttttaaa cagcgcgaat gcatcgaggc ccagcagggt
     9841 tgtccagctc tgattcgctt gctgtttagc aaccggaata tgcggcagag tcagggtaaa
     9901 cagaaccaga atggcggaaa gtgctgcgcc aatatacagc tgcatgtggc ttaattcgaa
     9961 gccagacagg ctcaccaccc acattgccat gataaagccg atggtgcccc agatacggat
    10021 tggcgggaag tcagtaacga tatccatccc ggcattttgc aggcgatagt aagagatggt
    10081 gttgattaac ccaagcgttg gcatataagc aaacgagtta atcaatatca caaggaacat
    10141 cgcctccggt gtcgtgacct gtgccgccat gaacagcgtg atagcgccaa tggtgtggca
    10201 aatggcatat acccatttcg cacttaacca tttgtcggcc acaatcccca gcagcgcagg
    10261 cataaagacc gcggcgatac ccagtgagct ataaactgcg ccaatagaag caccgtcaaa
    10321 cttcagggta acaaacatat aggagccgag ggtcgtcagc caacttcccc acagacagaa
    10381 ctgcagaaaa gagaggattt tcagctgcag cttaagattc atgttaattt cctcacatcg
    10441 tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc gagatgatgg gattctatca
    10501 acttcgcgga ctggtttttt gttacctgtg gcaaataatt gcaaacattc ccaatttgca
    10561 aaacgaaatt gtgatgtgga taacattttt gcccctgagc atcgtcaggg gcggttaatg
    10621 gaattatcgg cggcggtagg atttttgcgc ggtattcact ttataaagat aacggcgaga
    10681 ttccgcagag ggatggcggg tcgtcagcgt ctgataaaca tcgcccggcg tcatggtgtt
    10741 aataatattg gcagcctgaa tcttatcatt cgaaaagact cgcagcacgc tgcctgcgcc
    10801 gccgttatag gcggtgatga cggcataacg ccgcgatgtt gggttatcaa ttccgccgag
    10861 ataaacattg ttcagcatcg ccagatacgc ggtgccggta tcaatattgc tggcaggatc
    10921 aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc gagcggaaca catctttccc
    10981 ggcagtatgt tgtaccacct gcattaatcc cagcgcatcg gaacggctga ccgcatacgg
    11041 gttaaaggaa gattcggtct gcataattgc cagaatcagc gactcatcaa cgccatattt
    11101 ccgtgacgcc tggcggacca tgccgagata tttgtgcgca cgtttatcaa ggtggttcgg
    11161 taccatgtta atggtgacgc tgtagatgat acgcagcccg ttattgcggc tttgcagacg
    11221 gtttttcagc agataatccg cgaagttgct tgcgcgacct tcccagcgaa tcggctgccc
    11281 ggtgttgtcc accacctgac cgtaaaggaa aggttctttc gaaatcgtaa tatcatcaac
    11341 gtcggaatag agatcgaccg aactcggatc gtcacccatc agcaacgttt tgataattgc
    11401 ccggcgcaaa tgcgcggcag gttctgtccc ggcgatagtt tcgatagtaa tcgtaccgtc
    11461 atcgaagttg atgtggctgc gggtctgata ttgatcggtg tatttcacgt agtccttagg
    11521 accggcgatc accacctctt tgaatcccca gatgttctca atattgtggg caaattgccc
    11581 catcagaata tcaaaaccgt tggtatcttt gacccaggct tcgttatagg tatcgccttt
    11641 tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa gccagcgcga gatatttttt
    11701 catcattccg ggtgcgtgtt gtgtttgttt ttatgcaagt tgccggaggc gtgctccggc
    11761 actgttttta ttttttatct tccggcgtat agccctcgat atgcacctct ttaccctcga
    11821 acaggaagtt gaccatctcc tgctcaagca gcttgcggtg ctcggcattc atcatgttga
    11881 gtttcttttc attaatcagc atggtttgct tgtgctgcca ctgcgcccag gcttctttgg
    11941 agatctcgtt atagatgcgt tttcccagct cgccggggta cagctgaaaa tcctgacctt
    12001 ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct cataaatcat cctctttatc
    12061 gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg taacaaacgc tccacgggag
    12121 ccgccaggcc aactgacggc ggttgcgcta agttatacca gagcgcattg ccttcatcca
    12181 tgcagccggt gaatgacgac acgggaagcc acataggcac aatatctaag tggaaatggc
    12241 tgaaggtatg gcgaaacgcg gtcagctgcg tcaggttatc ggcagcaatc tgccgttgcg
    12301 ccagccaatg ccgcaaactt tcttcgtcgg caaactgcgg gaaacagtat aaaccgcccc
    12361 acaatccgct cggcggacgc tgcgccagca atacttcatc ttcgtgctgt aatagcaaaa
    12421 agtagccggt gcgctccggc agcgtctgtt tcggtttttt gcccggataa agcgaccagc
    12481 tattgttggc ggcggcaata catccgtttt gtagcggaca gagcgaacat ttcggtttcg
    12541 agcgcgtaca aatcatcgcg cccaaatcca tcatcgcctg attaaaccgt tccacgccga
    12601 ccgcgggcgt cacctgctcg cttaaactcc ataatttatt ctcgacctct tttttcccag
    12661 gccagccgct tacagcatag cagcgcgcca gcacgcgttt gacgttaccg tcgagaatcg
    12721 gaaagtgctt acccagagaa agcgagagaa tcgcgcctgc ggtggaacgc ccgacgcccg
    12781 gcagtgctgc aacttcctca aaggtttccg ggaatttacc gccgtgtaag gtcgccactt
    12841 gttgtgccgc tttatgcaga ttgcgcgcgc gggcgtaata gccaagcccg gtccacaagt
    12901 ggagaacttc gtcgagcggc gcattggcga gatcggtcac cgtcgggaag cgcgccataa
    12961 agcgttcaaa ataggggata acggtcgcaa cctgagtttg ttgcaacatc acttctgaga
    13021 gccatacttt gtagggcgtc ttgtcaattt gccagggcag agtttttcgc ccgtatttat
    13081 cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg catggtcacc gaattcactg
    13141 ttgttggggg caagattgca gcacagcgac ggcagggtgt aaaccggaac tttccgcagc
    13201 cacggcctta tcatgcttgc atccggcaac taactttgga taatgcccgt tttcagaaca
    13261 ctttcacaag cgactaaatc tttatgaaaa acgacgtcat ttcaccggaa tttgatgaaa
    13321 acggccgccc actgcgccgt atccgtagtt ttgtgcgccg ccaggggcga ctgaccaaag
    13381 gccaggaaca tgcgctggaa aactactggc cggtgatggg cgttgagttc agcgaagata
    13441 tgctggattt ccccgcgctt tttggccgtg aagcgccggt gacgcttgag attggttttg
    13501 gcatgggggc gtcgctggtg gcaatggcta aagatcgccc cgagcaggac ttcctcggca
    13561 ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc tgcgtatgaa gagggcttaa
    13621 gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct gcataaaatg attcctgaca
    13681 attcattgcg catggtgcag ctctttttcc ctgacccgtg gcacaaagcg cgccataata
    13741 aacgccgtat cgttcaggtg ccgtttgccg aactggtaaa aagcaaactg cagctggggg
    13801 gcgtattcca tatggcgacc gactgggaac cttatgcgga acatatgctt gaagtgatgt
    13861 cttctattga cggttataaa aacctgtcag agagcaatga ttacgtaccg cgtccggcat
    13921 cacgtccagt gacgaaattt gaacaacgtg gtcatcgtct tggtcacgga gtatgggact
    13981 taatgttcga gagggtgaaa taatggcaaa gaaccgtagc cgtcgtctgc gtaaaaaaat
    14041 gcacatcgac gaattccagg aattaggatt ttcggtggca tggcgattcc cggaaggtac
    14101 atcggaagaa cagattgata aaaccgttga tgattttatt aacgaggtca tcgaaccgaa
    14161 caaactggcc tttgacggca gcggttatct ggcctgggaa ggtctgatct gcatgcagga
    14221 aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt aagtggctgg aagagcgcaa
    14281 actggatgag gtacgcacca gcgaactttt cgacgtttgg tgggactaag aaagcatacg
    14341 ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta tcatgcctgg aaagatgcac
    14401 gatcgagtag gcgggataag gtgtttacgc cgcatccggc atggaaaacg cgtactttgt
    14461 tatcaatctg gggccagcaa atgctggcct gattttttct tgagggaaga ctatgatgcg
    14521 caaaatgctg ctggcggcag cactttcagt gacggcaatg accgctcacg ccgactacca
    14581 gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg caaaccgtgc aggtgaaggg
    14641 cgaaaacggc aatctggtga tcacgccaga cggcaacgtg atgtataacg gtaagcaata
    14701 ttccctgaat gccgcccagc gcgagcaggc gaaggattat caggctgaac tacgcagcac
    14761 gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgaa aaagcccgta ttgcgctgga
    14821 taaaattatc gttcaggaga tgggcgaaag cagcaaaatg cgcagccgtc tgaccaaact
    14881 tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa acgcgcagcg atggcctgac
    14941 gtttcactat aaagccattg atcaggttcg cgccgaaggc cagcaattag tgaatcaggc
    15001 aatgggtgga attttacagg acagcattaa tgaaatgggc gcgaaagcgg tgttgaaaag
    15061 cggcggtaac ccattacaga atgtgctggg aagcctgggg gggctgcaat cctcaatcca
    15121 aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt ggcaaagatg tttgtagccg
    15181 cgttgtgact ctggaagata gccgcaaagc cctggtcggg aatttaaaat aatcctctat
    15241 tttaagacgg cataatactt ttttatgccg tttaattctt cgttttgtta cctgcctcta
    15301 actttgtaga tctccaaaat atattcacgt tgtaaattgt ttaacgtcaa atttcccata
    15361 cagagctaag ggataatgcg tagcgttcac gtaactggag gaatgaaatg gagtttttca
    15421 aaaagacggc acttgccgca ctggttatgg gttttagtgg tgcagcattg gcattaccca
    15481 atatcaccat tttagcaacc ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat
    15541 ctaactacac agcgggtaaa gttggcgtag aaaatctggt taatgcggtg ccgcaactga
    15601 aggacattgc gaacgttaaa ggcgagcagg tagtgaatat cggctcccag gacatgaacg
    15661 ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga ctgcgataag accgacggct
    15721 tcgtcattac ccacggtacc gacacgatgg aagaaactgc ttacttcctc gacctgacgg
    15781 tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg tccgtccacg tctatgagcg
    15841 cagacggtcc attcaacctg tataacgcgg tagtgaccgc agctgataaa gcctccgcca
    15901 accgtggcgt gctggtagtg atgaatgaca ccgtgcttga tggccgtgac gtcaccaaaa
    15961 ccaacaccac cgacgtagcg accttcaagt ctgttaacta cggtcctctg ggttacattc
    16021 acaacggtaa gattgactac cagcgtaccc cggcacgtaa gcataccagc gacacgccat
    16081 tcgatgtctc taagctgaat gaactgccga aagtcggcat tgtttataac tacgctaacg
    16141 catccgatct tccggctaaa gcactggtag atgcgggcta tgatggcatc gttagcgctg
    16201 gtgtgggtaa cggcaacctg tataaatctg tgttcgacac gctggcgacc gccgcgaaaa
    16261 acggtactgc agtcgtgcgt tcttcccgcg taccgacggg cgctaccact caggatgccg
    16321 aagtggatga tgcgaaatac ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc
    16381 gcgttctgct gcaactggct ctgacgcaaa ccaaagatcc gcagcagatc cagcagatct
    16441 tcaatcagta ctaatcacct cgccccggta tcgtgccggg gctttttcac ttcagactta
    16501 cgtccattgc caattttcat taccctaatg ataatcaccg gaataaatta ttccgcgtga
    16561 ggtttttcgg gtgaaaaagc aatggattgt tggcacggca ctgcttatgt tgatgactgg
    16621 taatgcctgg gcagatggcg aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa
    16681 gcagtatcac ggcattctca agcttgatgc tatcacctta aaaaatcttg atgctaaggg
    16741 taatcaggcc acctggtcag cggaaggcga tgtctcttcc agtgacgatc tctatacctg
    16801 ggtcggtcag ttggcagatt acgaactgct cgaacagacc tggacgaaag ataaaccggt
    16861 aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg tctggctggt cggtgaactt
    16921 ttactctttt caggcggcag ccagcgatcg tgggcgggtg gtcgacgata tcaaaacgaa
    16981 taataaatat ctgatcgtga atagcgaaga tttcaattat cgctttagtc agcttgaatc
    17041 tgcgttgaat acccagaaga actcgattcc tgcgctggaa aaggaagtga aagcgcttga
    17101 taagcaaatg gtggcggcgc agaaagcagc ggatgcatat tggggaaaag atgcaaacgg
    17161 taagcaaatg acccgcgaag aagcctttaa aaaaattcac caacagcgtg atgagtttaa
    17221 taaacagaac gatagcgagg cgttcgcggt taaatatgac aaagaggttt atcaaccggc
    17281 gattgcggca tgtcataaac agagtgaaga gtgttatgaa gtgccgattc agcagaaacg
    17341 agatttcgat atcaacgaac aacggcgaca gacttttctg caatcacaaa aacttagccg
    17401 taaattgcag gatgactggg taacgctcga aaaagggcaa tatcctttaa cgatgaaagt
    17461 ttcagaaata aacagtaaaa aagtcgcgat tttgatgaag attgacgata tcaaccaggc
    17521 taatgaacgc tggaaaaaag ataccgaaca actgcggcgc aatggggtga ttaagtaagt
    17581 ttgatatagt gcacgatttg tagcaggatg cggcgttgac cccgcatccg gcaatcaacg
    17641 tctgatgcga cgcttgcgcg tcttatcaga cctacaaccg ttgccacatg taggctggat
    17701 aaggcgttca cgccgcatcc ggcaatacaa gtttactcag ccagaaaaag ctccagcagc
    17761 gaatttaaaa acagcttccc atgttccgtt atctgccagt aatccgcaca ttcggtgaga
    17821 taaccctggg caatcgcctc gtctaactgt gggcgaatca catcttcgca aagcccggta
    17881 tacgcaataa actccacgcg cggcgcggcc tccagcagac ggaagcgatt cataaagaac
    17941 tcaaacggct tatctgcggc ttcgacatca cgctggcttt ccagatacct tccttgcata
    18001 aaaccacgcg gatgacgcgt tttagtggta cgcagaatgc gcccatccgg gaaggtcact
    18061 ttgccgtgcg cgccgcagcc aataccgatg tagtcgccaa agcgccagta gttgagattg
    18121 tgctggcact gataactggg tttggcgtaa gcggaagttt catattgctg ataacccgct
    18181 gcggttaata actgatgccc ctgttcgaaa atatcccaca gcgcgtcatc gtccggcagc
    18241 accggtggtc gcgaaccaaa cagcgtattg ggttcgatgg tcagttgata ccaggaaaga
    18301 tgcggcggat tcagttcaat ggcctggcgt agatcgccaa gcgcctcttc cagtgattga
    18361 tccggtagtc catgcatcaa atcaaggtta aagctacgta accctaaacc gctcgccagc
    18421 tttgccgctc gtttcgcttc ttgcgggcca tgaatgcgcc cgagtcgttt cagcttttct
    18481 tcgctaaaac tctgcacacc aatagagatg cggttcacac cagcacgctg ataatcgaca
    18541 aagcgatccg cttctaccgt gcctgggttc gcttccatag taatttctgc atccgctgcc
    18601 agcggcaaac gcgcacgcac gccgtccagc agcgtttgca tcgccgggcc ggaaagcagg
    18661 ctcggcgtac caccgccaat aaagattgtc tttacttcac ggccctgagc gtaagccaca
    18721 tcgttgtcca gatcgttaag cagatgctga acataatcgt cgtgcggcac ttctcctttc
    18781 aacgcgtgag agttgaaatc gcagtacggg catttctgca cgcaccacgg gatgtgaatg
    18841 tagagactca gcggcggtaa tttaaccatt acgtaaagcg tccagcagca gtttcaatgc
    18901 ctgaccacgg tgggaaatgg cgctcttttc ttcgcgggtc agttcggcgg cggttttccc
    18961 ttcggaaggt acgaagaaga ttggatcata accaaagcca ccagtgcccg ctggttcacg
    19021 agtaatcacg cccggccagc taccgtggca taccagcgga gtcggatctt ccgcgtgacg
    19081 cagatatacc agcacgcagt ggaaacgcgc ctgacgttgg tcatccggta cgtctttcag
    19141 tgtttccagc agtttttgca gattctttag atcggtcgcg tcttcaccgg aataacgcgc
    19201 ggagtaaatg cccggcgcgc cgccaagcac atctaccgcc agaccagagt cgtcggcaat
    19261 tgccggtaaa ccggtcactt ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa
    19321 ggtcaggccg gtttcttcag cggaatctac gccgaggtct gtttgggcca cgatatcaag
    19381 accgaagtcg ctaagcagcg acgccagctc acgcacttta ccggcattgc cggttgcgag
    19441 gacaactttt tgcataggat acctaattaa ttaacgccgc gatttctggc gggatttgtt
    19501 gcggattaat gattttaatt tgtttgtggc ggccaagttc gcctttttca atcaccacct
    19561 ggcttttggc aacccggaat tgcttaccga gaaacttcac cagatgactg ttggcctgac
    19621 cgtcaaccgg cggcgcggta atggcgactt taacttcgtc gccatgtaaa ccgacaatag
    19681 aatcacggct ggctttcggc tgaatataga gccgtaaaac cagaccgtca tcattaactg
    19741 ttacggcact cataacgcca tccacagccc cggcagcagc atgtttccgg ttgcctgtaa
    19801 tacttctgcg acacccatat tgatgacata cagcagcaga acgaggatca tcggcgagaa
    19861 atcaatccca cccattgccg gtagcaggcg gcgaatcggg cgcagcagcg gatcggccag
    19921 ctgaatcagc acgtattcaa tcggactacg accctggctt acccagctca taatcgccat
    19981 caccagcagg acccagaaaa tcagcaggcc gatggttttc agcagaatca gtaaaccggc
    20041 aatccagatg attggcagga aggtcaccac tttaaacagc acgatggctt tgataaaact
    20101 gagaatgtac gcaaccagca gcgaggcgct atcaatggga cccattgccg gaataacgcg
    20161 gcgcagtggc ccgataattg gctgtgttac cttcactaca aactgtgaga aggggttgta
    20221 aaaatcacaa tgagcccact gcatccagat gcgtaataac agcaccatgg tatacagctc
    20281 aatgaccgtt gaaagcagga aagtcaacgt attcatggcg ttcctttaat tccttatttt
    20341 ttagagtaat cacgcgcacc aaaaattgca gtgccgatac gaaccatcgt gctacctgcc
    20401 gcaatggcgg cttccatatc gtccgacatt cccagagaga gcgtgtcgat atgcgggtag
    20461 cgcgttttca gtccggcaaa tgctacagcc atttggcgtg caacttcaaa ctgccttaca
    20521 tattctgact caggcgcagg gattgccatc agcccgcgca gacgtaaacg cggtagttca
    20581 gcgaccgccg ctgccagctc gtccagttct gccagttgaa tcccggactt actgttttca
    20641 tcactaatat taatttgaat cagtacgtta agagggggaa gttctgccgg gcgctgatcg
    20701 ttgaggcggg tagcaatgcg caaacggtcg atggtatgac accagtcgaa atgctctgcc
    20761 accaggcggc ttttattaga ctgcaacggg ccaataaaat gccattctaa tcctgttacg
    20821 cccagttcct gaaagtggcg aattttatct accccttcct gaacgtagtt ttcaccaaat
    20881 tgacgctgcc cggcatcaat ggcttctgcg atggcgctcg caggttttgt tttactgact
    20941 gcaagcagcg taatttcttc tggagaacgg ccgcaacgcg ttgcagccgc tgagattttg
    21001 tcccggacct gtgccaggtt atgcgcaata tcgttcattt tccgaggatc ttagtatgaa
    21061 tatggaagaa attgtggccc ttagtgtaaa gcataacgtc tcggatctac acctgtgcag
    21121 cgcctggccc gcacgatggc gcattcgcgg gctaatggaa gctgcgccgt ttgatgcgcc
    21181 ggacgtcgaa gagctactgc gggagtggct ggatgacgat cagcgggcaa tattgctgga
    21241 aaatggccag ctggattttg ccgtgtcgct ggcggaaaac cagcggttgc gtggcagtgc
    21301 gttcgcgcaa cggcaaggta tttctctggc attacggttg ttaccttcgc actgtccaca
    21361 gctcgaacag cttggcgcac caacggtatt gccggaatta ctcaagagcg agaatggcct
    21421 gattctggtg acgggggcga cggggagcgg caaatctacc acgctggcgg cgatggttgg
    21481 ctatctcaat caacatgccg atgcgcatat tctgacgctg gaagatcctg ttgaatatat
    21541 ctatgccagc cagcgatgtt tgatccagca gcgggaaatt ggtttgcact gtatgacgtt
    21601 cgcatcggga ttgcgggccg cattgcggga agatcctgat gtgattttgc tcggagagct
    21661 gcgtgacagc gagacaatcc gtctggcact gacggcggca gaaaccgggc atttggtgct
    21721 ggcaacatta catacgcgtg gtgccgcgca ggcagttgag cgactggtgg attcatttcc
    21781 ggcgcaggaa aaagatcccg tacgtaatca actggcaggt agtttacggg ccgtgttgtc
    21841 acaaaagctg gaagtggata aacaggaagg acgcgtggcg ctgtttgaat tattgattaa
    21901 cactcccgcg gtggggaatt tgattcgcga agggaaaacc caccagttac cgcatgttat
    21961 tcaaaccggg cagcaggtgg ggatgttaac gtttcagcag agttatcagc agcgggtggg
    22021 ggaagggcgt ttgtgacgtt gccatgcccg atgcgcaagt ttatagggcc tataaaggca
    22081 ataaatgtct gatgcgacgc taacgcgtct tatcagacct acagccgctg ccagatgtgt
    22141 gctggataag gcgttcacgc cgcatccggc aagatggcgg taacgactat ccgccaggct
    22201 tttagatcgc cttaatatcc ctgctcgaaa tagctttcga gaataataac cgcagaggca
    22261 gagtcgactt tgcctttgtt gagcgcccga tagccgccct gttcaaacag accggaacgg
    22321 gcttccacgg tgctaagacg ttcgtcatgg agctttactt caacaccgaa acgaccatga
    22381 atacggttgg caaatttacg cgctctggca gtcaatggtt gctcggtgcc gtccatattc
    22441 agcggcaaac cgacgatgat ttcgtccggc tgccactctt tcagtaaacg ttcgatgagg
    22501 ttccagtccg gcgtaccgtc ctgtgcttta attgcaggca aagggcgagc agtgccggta
    22561 atgcgttggc cgaccgctac gccaatgctt ttggtgccga agtcgaaggc gagtaaggtt
    22621 ccactcatca ggcgtgccct gccacaccag gcatggtgag aatatccaca ccaatcagtt
    22681 ttgccgcctc gcgccagcgg tcggcaatcg gcgttttgaa cagaatattc agatccgccg
    22741 gggccgttag ccacgcgtta tcgagaattt cttgttccag ttgacctttt tcccaggagg
    22801 cataacccag agccaccaat acgtcagacg gttgtttatc ggtgccgagc gtttccagca
    22861 catcgcggga agtggtcatc actgtgttgt ctgaaatgcg aatgctggaa gcaaaattgg
    22921 agggcggagt atgcaaaata aacccgcgat cttcagccag cggaccgccg agcataaccg
    22981 gtttatccag acggattgat tcatcacgcg gctccggcgt gatcttcagc ttttccagaa
    23041 tcccttcaat tttgagattt tccagcggct tgttgacgat gatccccatt gcaccattgg
    23101 tattatgttc gcaaatgtag accacggaac gacggaaaat cggatcctgg agagcaggca
    23161 tggcaataag aaagtgatgc tgtaaattca ttgtcagagg ttctgtttcc tggttcaaaa
    23221 aagcaacagc gcccagtatg cgtggaaaga agggcgctgt cactcagagt ctcaacgaga
    23281 tccttctcgc taaggtgggt tactgctgct gtaaacgtgc ttcgatggca tccattaaca
    23341 ttccggtgat cgacaccgga aactctgctt caatctcacg aatacaggtt gggctggtga
    23401 cgttaatttc agtcagacgg tcgccgatga tatccagacc aacaaaaatc agcccttttt
    23461 ctttcagcgt cggcccgatc tgacgggcga ttttccagtc actttccgtc agcggacgag
    23521 gttcaccgcg accaccggca gccagattgc cacgggtttc gcccccctgc ggaatacgcg
    23581 ccaggcagta cggtaccggc tcgccatcca ccaccagcac gcgtttgtcg ccatctttaa
    23641 tggctggcag gtaattttgc gccatgcagt agcgagtgcc atgctcagtc agggtttcgg
    23701 caatcacgcc gaggtttgga tcgccttctt tcacgcggaa aatcgacgcg ccgcccatac
    23761 cgtccagcgg cttaagaatg atgtcgctgt gtttctccca gaacgctttt agctgcgctt
    23821 tattgcgcgt aaccagcgtt tctggcgtta agtcagagaa ccaggcggta aacagtttct
    23881 cgttacagtc gcgcaggctc tgcggcttgt taacgatcag cgtccctttc tcttcggcac
    23941 gttccagaat ataggtcgcg tagataaact cggtatcaaa cggcgggtct ttacgcatca
    24001 ggatcacatc gagatcggcc agcggcagat cctgttcacc gacgaacgaa aaccactctt
    24061 cgtagttctg cttcacgttc agcgtgcggg tatgggcgcg ggcttcgcca ttgatcagat
    24121 acagatctcc catctccata tagtgaagtt cgtaaccacg acgctgtgct tccagcaaca
    24181 tagcgaaact ggaatctttc ttgatgttga tgtttgcgat ggggtccatc acgatgccga
    24241 gcttgatcat tattcttctc cgttagccca aatcgccaaa tcgtacttgt agcgcggtaa
    24301 tggcggtgag cgcagttgtc tctgtacgca aaacgcgagg tcccaacagg atatcagtaa
    24361 attgatagcg ggcagtcatg gcaatttcat ctgccgataa accgccttcc gggccaatca
    24421 gcaggcggac gcgttcaacc ggtaacggca acgtattgat gctgttactg gcgcgcgggt
    24481 gaagattcag tttcagtcct tcatcctgct ctgcacacca ggcttccaga tccatcgctg
    24541 ggcggatttc cggcacccgg ttacgaccac actgctcaca ggcagcaatt gcaatcttct
    24601 gccactgctg gagcttcttg ttcagacgtt cactatccag tttaacgccg cagcgctcag
    24661 aaaaaagtgg cgtaatgagg cttacaccga gttcgatcga tttctggatc gtaaattcca
    24721 ttttttcacc acgcgacatc acctgaccga ggtgaatatg cagcggagat tcgcgatcgt
    24781 cgatcttgcc ttccagcacc ttcacttcca cgcttttttt gctggcgctg gtaatttcgg
    24841 cgtcaaagac ctggttgcta ccgtcaaaca attgcaacgc ctgccccggc cccatgcgca
    24901 gtacgcgccc gatatggttg gcggcatctt cgcaaagcgc gatgtgagaa tggctggtca
    24961 gtggttcagg atgataaatg cgggggatac gcatagttaa aaatccgcgt cgtctcccca
    25021 cgcggttgta cgcgtggggt aggggttaac aaaaagaatc ccgctagtgt aggttagctc
    25081 tttcgcgcct ggcaagcgcg ttgcacatac gggttatgat tgccttgcac cttcgcgatg
    25141 cgttcatcgc gctcgcactc ccagtcggta accggataca tcttgttcca tgcgttgaac
    25201 agctgcgttt gctggcgaga gagtgtcagg ttgtattggt cgcgcatata gaagtaggtg
    25261 cgcgcaatgg caccgcgtgc acgcgctggt ggttcggcag ctttttcttt gaaatcgacc
    25321 ttcatggcgc attgaccgta ctggccttcg ccgccattcc actggctgta cataaagttg
    25381 ccgcgatcgc cattcacctc accgactgac ggctgcaggt tatgcatatc gctttccatc
    25441 ttgcgataga ccggatcttt agcgcagttt ttacgtccac cgtcctgcca gcactggcgc
    25501 tggtgaccga actgccaggc gggaacgacg tgttcccact ctacgcggct ggctcggttt
    25561 tcatttttgc gcacctgata gccgcacgat tgcagatcaa caacgccttt tttgccctgc
    25621 cagttaattt tacatccgca ataaaacgta ccgggcgcgt cagcgtggac ttttaccgcc
    25681 gcggctttcg cctgagaaaa actattgata ccttcggcca acgccgggcc ggaaaatgct
    25741 gcgctcagta ccaccgcagc aatagacaaa taacggtaca tcacttactc cgtgttaaaa
    25801 cgagccagca acgtagcgaa agttgttttg gtatgcaatc agccagatca ggaaagttcc
    25861 tgatagttca gatggttatt tcgcaaccag ctgttcaccg cagtgaacac agcgatagac
    25921 ggcctcgcca cgaactacgc gattatggcg gcgtacggta agctgatgct cctggcactt
    25981 gcagcggtag gggaaggtgt tgcgacgcac ggattgcagt tcgaactgat gcgtacgacg
    26041 ggcgggaacg cccagcacgc tttccatcat ccacttccac tctttgccat gtggcgctac
    26101 gcggccgaaa tgtttccata ccagcaaatg tgccagttcg tgcggtacca cttcttcaat
    26161 aaaagcttca ctgttttcca acagcaaaac gggattgagg cggatttcat agctttccag
    26221 ccaggctgtt ccggcggagg ttccgcgctg ggtgtaagag agttttggct ccgggtagtt
    26281 acgccctagc ttcaggttgg cctgggcgag tttttcccgc aggcggcgca taacggcctg
    26341 ctggatggcg atagggagac gggatgtttt catagcggca gaggatagag cgaagaaaag
    26401 atgactgcaa gaggtggctt cctccgcgat gggaggaagc ctgaggagat taatcgtgag
    26461 cgccgatttc gcgcagttta cgacctttca tcagattacg ttcaatatgt tccagcgaaa
    26521 cgtgtttggt ttccggtacc agccacaatg tcagcaggat aaacagtacg ttcagagccg
    26581 catacaccca gaaggtgttg gcgttaccca gcgtgttgag catggtcagg aacgttgcgc
    26641 caacgatcat gttggcaatc cagttggtgg cggtggagca ggtgatgcca aaatcgcggc
    26701 ctttcagcgg ctgaatttcg gagcacagta cccaaatcag cggaccggca ctcatggcaa
    26761 aaccgacaat aaacatcagc agcatggcga tggcgaaata ctgcgctgac ggagagtgaa
    26821 taccgatatg catcattgta ccgagtacgc ccatgccagc tgccatcacc aggaagccca
    26881 gcgttagcgt tggtttacgt ccccagcggt caacaaggcc gattgcgata aaggtggcaa
    26941 gtacgttggt caggccgaca atcacggtcc cccacatttg ctcggtagtg ttggtataac
    27001 ccgccagttc gaagattttc ggcgcgtaat acatgatgac gttcatcccg gtgaattgct
    27061 gcattacctg caacagtacg ccaaggaaca ccgcgcggcg gaagttgctg ttctctttaa
    27121 acagcgccca gccactctgt ttaacctgca aactttcacg gatttcatcc agttcgcgtt
    27181 tcgcttccgc gctggtgtca cgcaggcgta gcagcacgcg ttcggcatca acaaaacggc
    27241 gcttggcggc aaaccaacgt gggctgtctg gcaggaagaa gacaccaatc agcagcaaaa
    27301 ttgccgggat gataatcaca cccagcatcc agcgccatgc accggtgtag ctgaaggcgg
    27361 tatcagaaag ataagcaccg aggatcccga tagtgatcat caactgatac atcgagatca
    27421 tactgccgcg aattttttcc ggcgcaattt ctgacaggta cagtggtgcg gtataagagg
    27481 ccacacccac cgccagcccc agcagaacgc gggaaagaat cagtacttca acgtttggcg
    27541 cagccgcaga gaacagcgaa ccggcaacaa acaaaattgc gccgatcatc aggctctttt
    27601 tgcgcccgag tttaaaggag agccagccgc tgcccaccgc accgactgcc gcaccgaaca
    27661 tcatggagct tacgacccat tcttgcgtat gcgaagtaat ctggaattca tctgcaataa
    27721 acggcagtgc gccagcaatt acaccgatat ccaggccaaa gagtaatccc gccagagcgg
    27781 caaggaagca gacgaaaaac gtcattgcct tgtttgaccg cccctgtttt ttagcgtcag
    27841 gcatgatgcc ctccaatatg gttatttttt attgtgaatt aagataggtg agtacgacgt
    27901 aaaaagatgt gaagcaaatc acatcagtgt aatcgcttac actaacattt atcttagtta
    27961 tggtttaagt gcatgaaaat aattgag
//
LOCUS       DAPHDX010000055        27755 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPHDX010000055 DAPHDX010000000
VERSION     DAPHDX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.055"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(474..1106)
                     /locus_tag="RH058_002279"
     CDS             complement(474..1106)
                     /locus_tag="RH058_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV3137865.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1440..1715
                     /gene="ypdI"
                     /locus_tag="RH058_002280"
     CDS             1440..1715
                     /gene="ypdI"
                     /locus_tag="RH058_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDV3137866.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1792..2034)
                     /locus_tag="RH058_002281"
     CDS             complement(1792..2034)
                     /locus_tag="RH058_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV3137867.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2387..3307
                     /gene="lpxP"
                     /locus_tag="RH058_002282"
     CDS             2387..3307
                     /gene="lpxP"
                     /locus_tag="RH058_002282"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV3137868.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3663..3734
                     /gene="ypdK"
                     /locus_tag="RH058_002283"
     CDS             3663..3734
                     /gene="ypdK"
                     /locus_tag="RH058_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV3137869.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3799..5037)
                     /gene="alaC"
                     /locus_tag="RH058_002284"
     CDS             complement(3799..5037)
                     /gene="alaC"
                     /locus_tag="RH058_002284"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV3137870.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            5414..7111
                     /gene="ypdA"
                     /locus_tag="RH058_002285"
     CDS             5414..7111
                     /gene="ypdA"
                     /locus_tag="RH058_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV3137871.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            7126..7860
                     /gene="ypdB"
                     /locus_tag="RH058_002286"
     CDS             7126..7860
                     /gene="ypdB"
                     /locus_tag="RH058_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV3137872.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            7873..8730
                     /locus_tag="RH058_002287"
     CDS             7873..8730
                     /locus_tag="RH058_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3137873.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(8733..11228)
                     /gene="ptsP"
                     /locus_tag="RH058_002288"
     CDS             complement(8733..11228)
                     /gene="ptsP"
                     /locus_tag="RH058_002288"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3137874.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQVRRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(11253..12290)
                     /gene="ypdE"
                     /locus_tag="RH058_002289"
     CDS             complement(11253..12290)
                     /gene="ypdE"
                     /locus_tag="RH058_002289"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3137875.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(12290..13375)
                     /gene="ypdF"
                     /locus_tag="RH058_002290"
     CDS             complement(12290..13375)
                     /gene="ypdF"
                     /locus_tag="RH058_002290"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3137876.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(13390..14637)
                     /locus_tag="RH058_002291"
     CDS             complement(13390..14637)
                     /locus_tag="RH058_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV3137877.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(14659..14985)
                     /locus_tag="RH058_002292"
     CDS             complement(14659..14985)
                     /locus_tag="RH058_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDV3137878.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(15204..16169)
                     /gene="glk"
                     /locus_tag="RH058_002293"
     CDS             complement(15204..16169)
                     /gene="glk"
                     /locus_tag="RH058_002293"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV3137879.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            16373..17629
                     /locus_tag="RH058_002294"
     CDS             16373..17629
                     /locus_tag="RH058_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV3137880.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            17744..18070
                     /locus_tag="RH058_002295"
     CDS             17744..18070
                     /locus_tag="RH058_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV3137881.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(18211..19449)
                     /locus_tag="RH058_002296"
     CDS             complement(18211..19449)
                     /locus_tag="RH058_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV3137882.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            19785..20987
                     /gene="nupC"
                     /locus_tag="RH058_002297"
     CDS             19785..20987
                     /gene="nupC"
                     /locus_tag="RH058_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV3137883.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(21037..23226)
                     /locus_tag="RH058_002298"
     CDS             complement(21037..23226)
                     /locus_tag="RH058_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV3137884.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(23434..23509)
                     /locus_tag="RH058_002299"
     tRNA            complement(23434..23509)
                     /locus_tag="RH058_002299"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23474..23476),aa:Ala,seq:ggc)
     gene            complement(23549..23624)
                     /locus_tag="RH058_002300"
     tRNA            complement(23549..23624)
                     /locus_tag="RH058_002300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23589..23591),aa:Ala,seq:ggc)
     gene            23845..24204
                     /locus_tag="RH058_002301"
     CDS             23845..24204
                     /locus_tag="RH058_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV3137885.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            24206..24598
                     /locus_tag="RH058_002302"
     CDS             24206..24598
                     /locus_tag="RH058_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV3137886.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(24650..26065)
                     /gene="gltX"
                     /locus_tag="RH058_002303"
     CDS             complement(24650..26065)
                     /gene="gltX"
                     /locus_tag="RH058_002303"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV3137887.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            26324..26399
                     /locus_tag="RH058_002304"
     tRNA            26324..26399
                     /locus_tag="RH058_002304"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26357..26359,aa:Val,seq:tac)
     gene            26444..26519
                     /locus_tag="RH058_002305"
     tRNA            26444..26519
                     /locus_tag="RH058_002305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26477..26479,aa:Val,seq:tac)
     gene            26524..26599
                     /locus_tag="RH058_002306"
     tRNA            26524..26599
                     /locus_tag="RH058_002306"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26557..26559,aa:Lys,seq:ttt)
     gene            26630..26755
                     /locus_tag="RH058_002307"
     ncRNA           26630..26755
                     /ncRNA_class="other"
                     /locus_tag="RH058_002307"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(26864..27748)
                     /gene="xapR"
                     /locus_tag="RH058_002308"
     CDS             complement(26864..27748)
                     /gene="xapR"
                     /locus_tag="RH058_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDV3137888.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT         6967 a         7271 c         6738 g         6779 t
ORIGIN      
        1 cgtttattta aggcgtaata tcttctataa ataacccgca aaatatcgtc gtagctgcac
       61 tcgatatttt taaaatcctg tatcaagtgc tgaaaatcat ttcatcgcca cttccaattc
      121 ctttctgcat ggtaagtagt gaggacgaaa agaagaatat gataaatatt gattcgtggt
      181 aatcagagag agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga
      241 tttacaagta agggtatgat tacgcttttt aaagcgtacc ataaaacata acataataaa
      301 tgatttgtga tgacacggga ttattgggat ttactttgag gaagataaaa tgcgtgttta
      361 tagaaccgcg agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa
      421 attgtcggct ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc
      481 acaaacagtg cttcgctgtc gacgatgatt ccatcttccg cacctttaag cgccaggttt
      541 gcctcgtagt attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc
      601 tgtttaagcg gcatcagata ttggttttcc attacgccaa caccggccag gcgaagttct
      661 tccattgcgc cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct
      721 tctttttcgg gagttgctac ataactctca gaaattccga cagatgcatt aatgacaata
      781 tattggtcat cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta
      841 ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttctggatcg
      901 ccatgaaaca gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca
      961 gagatacgct ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt
     1021 gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc
     1081 atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc
     1141 cgccatcata ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg
     1201 gttatcttac agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtagttat
     1261 tatcgcaaga agtattttag tcttacaatt gtaggaatct gcttcctgtc gcagagaact
     1321 attcttatgt accacaaaga gatgtcattt attatatcga tgtaaatatt gattaattat
     1381 atgtaaatac agccataaca tcgtaggttc tcagtgaacc attttcagga taactttcta
     1441 tgaaagtaaa cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg
     1501 tttttgcatt ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa
     1561 catcagcgat gacaaccaca cactctacaa aacttcagcc atcacaggcg attttgttta
     1621 agatgagaga agatgcgcca ccattaaacc tcacagaaga aatcacgcct acttatccga
     1681 caaaggcgaa ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa
     1741 tcatgtctga tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct
     1801 gccatacccc accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat
     1861 ccccgtcact agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc
     1921 gaggatcacc atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat
     1981 gtagccgctt acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca
     2041 acctcctgcc gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta
     2101 acctctggca atttttgcta agcatcaatg aaaacagcat gttaaatgca agactgttgt
     2161 gtacggaaaa atatttactt tgcacgatta ataatcagta gctgaaagca gtcagcgagt
     2221 gataaagtac agataccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac
     2281 tctttacagg aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac
     2341 atggaatctt ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg
     2401 caaattttcc cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt
     2461 actctggctt tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgcattgg
     2521 cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact
     2581 ttgtttcccg cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc
     2641 actcggcatg gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg
     2701 taaatggttt gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg
     2761 cgtaatggtt gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact
     2821 gtgccaacca atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca
     2881 gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat
     2941 tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg
     3001 taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac
     3061 ctatgttctc tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc
     3121 ggattacagc ggatatcgtt tgttcatcac cccagagatg gaaggctacc cgacagatga
     3181 aaatcaagcc gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga
     3241 gcagtacctc tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta
     3301 catttaacaa actgctctta ctctttccct cctcaaatcg gccataataa cctcatggtt
     3361 tttatggctt ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa
     3421 attaaactgt cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgcct
     3481 cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat
     3541 tctggggtga tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac
     3601 tcactgtatc aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta
     3661 aagtgaaata tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg
     3721 ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg
     3781 catttctgac gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag
     3841 taaaccgtcg gcacggaaca tcgctttaat cccacgaatc gcctgacgaa tacggtcgcg
     3901 gttttcaatc agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg
     3961 cgagacacaa accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc
     4021 cgcatatggt tccgggattt tcgcccagac atacatcgaa gccttcggca tttcaaccat
     4081 ccagcccgct tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggc
     4141 aatgtcgcgc acgcactgtt gatcgccctc cagcgccgca atcgccgcca cctgcaacgg
     4201 cgtaaaggtg ccgtaatcgt gatagctttt aatacgagcc agggcgctga ccagcgtttt
     4261 gttgccaacc ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa
     4321 gaactcgact gccacatcgc gtgcacccgg tacctgcatg attgacggcg ctttccagcc
     4381 atcgtagacg atatcggcat aggccaggtc gtggaccacc agcacatcgt agcgtttcgc
     4441 cagcgccaca accttttcga agaactccag ctccacgcat tgcgcggttg gattcgacgg
     4501 gaagccgagg atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc
     4561 gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac
     4621 cgcgccataa atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc
     4681 cagcgtcgcc agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc
     4741 tgattccggg tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg
     4801 taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt
     4861 acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc
     4921 gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac
     4981 gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat
     5041 ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt
     5101 tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatcaatgg
     5161 ctttaaatga ggaactccac ttcaaaaaca ggaaattttt ctacgctaac aagaaaatta
     5221 ccaccaattt tattaacatt ataatttcaa taaatttacc agccagaagt gcattatcaa
     5281 gagatacatt gctaatacac catttatctt tcaatcccct tttaaattta tggtttttcc
     5341 tcaaaaacgg cctgctggtc atcagccttc aggttaccta tcatagaggt ttaatcctta
     5401 ttcagagtca cccgtgcacg aaatattcaa catgctgctg gcggtcttcg atcgggcagc
     5461 gttaatgctt atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg aactgttgca
     5521 caagtcggcg cactccccaa aagaattgct cgccgttacc gccattttct cgctgttcgc
     5581 cctgttcagc acctggtccg gcgttcccgt agaaggctcg ctggtgaacg tacgtattat
     5641 cgcggtgatg tccggcggga ttctgtttgg cccgtgggta ggcatcatta ccggcgtgat
     5701 tgcgggtatt caccggtatt taattgatat cggcggcgtg acggcgatcc cctgctttat
     5761 caccagcatt ctggcgggtt gtatatcagg ctggataaac ctgaaaatcc ccaaagcaca
     5821 gcgctggcgc gtcggtattc tcggcggcat gttgtgtgag acgttgacca tgattctggt
     5881 aattgtctgg gcaccaacta ccgcgttggg gatcgatatc gtctctaaaa tcggcattcc
     5941 aatgatcctc ggtagcgtct gtatcggctt tattgtgctt ctggtgcaaa gcgttgaggg
     6001 cgaaaaagag gccagcgccg cgcggcaggc caagctggcg ctggatatcg ccaacaaaac
     6061 gctacctctg tttcgccatg tcaatagcga gtcattacgc aaagtttgtg aaattattcg
     6121 cgatgacatt cacgccgatg cggtggcgat taccaatacc gatcatgtgc tggcctatgt
     6181 tggcgtgggt gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca
     6241 ggcgatgaat tacggaaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga
     6301 gattcattcc atgctggtga tcccattgtg ggagaaaggc gttgtgaccg gaacgctgaa
     6361 aatttactac tgccacgcgc atcagatcac ctcgtcatta caggaaatgg cggtcggtct
     6421 gtcgcaaatc atctccaccc aactggaggt ttcacgcgcc gagcagctac gtgaaatggc
     6481 aaataaggca gagcttcgcg ccctgcaaag caaaattaat ccccatttcc tgtttaacgc
     6541 tttgaacgct atttcatcat caatccgtct gaatccggat actgctcgcc agctgatctt
     6601 taatctgtcg cgttatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat
     6661 caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggtga
     6721 caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct
     6781 gatccagccg ttggtggaga acgccattgt ccacggtatt cagccttgca aaggtaaagg
     6841 cgttgtcacc atcagcgttg cagagtgcgg aaatcgggtc cgcattgcgg tgcgagatac
     6901 cgggcacggc atcgatccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa
     6961 taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca
     7021 tatccgccgc ctggagccgg ggacggaaat tgcgttttac attcctaacc aacgcacccc
     7081 agtcgcctca caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt
     7141 gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag
     7201 atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac
     7261 cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggcgt gttgctggcg
     7321 caaaacatca gccagttcgc ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa
     7381 catgcggtag aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag
     7441 tcacgtatta ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc
     7501 agtacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag
     7561 cgaataatcg tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc
     7621 tttgtctata cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc
     7681 aaactgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa
     7741 atacgcgaaa tcgaaccgtg gtttaataac acctacattc tgcgactgaa agatctggat
     7801 tttgaagtgc cggtcagccg cagcaaagtg aaagaatttc gccagttaat gcatctttaa
     7861 tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg
     7921 ttcagcggcc tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct
     7981 gcctcgttac cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc
     8041 ggcgaatacg gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg
     8101 tttattccgg cacgcgcggc taatttaccg atcaacaaca aaccggtgat gctgttaagc
     8161 ctggtgttcg caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg
     8221 ttgcatcctg ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg
     8281 cttaccgccc tcacccatct tagccgttcg ccgctggagc agaatatcat tcagccactg
     8341 gtgttaagtt tgctgcatct ttgccgcaac gtggtgaata tgccgccggg taattcgcaa
     8401 ccgcgcggcg attttctcta tcacagcatt tgtaactggg ttcaggataa ttatgcccag
     8461 ccgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa
     8521 ctgtttgctc agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg
     8581 aaggcgagga tgattttgca gaaatatcat ctgtcaattc atgaagtggc acagcgttgc
     8641 ggttttccgg atagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg
     8701 ggagagtaca gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt ttccagcagc
     8761 gtcaggatac tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt tttattcacc
     8821 agtttacgcg ccagctgcga gaagactttc acatgattca tcccttcgtt agcacccagc
     8881 gtcagcatga tcaccagttc gacttcgccc atttctgact gccagtcaat cggtttcgcc
     8941 agccgggcaa tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgct
     9001 acgccaaaac caacgccggt ggtaacaatc tcttcccgct gccagacatc ttcttccagc
     9061 tcaaacggat gttcagtgcg cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc
     9121 tgctctttat tgctaaaatc ctgatcaaca aagatattct ccagcgccag cagtgggcga
     9181 acgtcttctt ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat
     9241 tcacatgcct gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt
     9301 ttcaccgccg gaatacgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt
     9361 agcggcagat aacggctttc accgcccagt tcaccgcaaa tgcctaccca tttgccccgc
     9421 tgatgcgcag tggtaacaat ctgctgcaac atgcgcagga acgatggcgt aatcgggtta
     9481 tacagcggcg atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg
     9541 ttagaaccga tactgaagaa atcgacctca tcgcagaagt ggtcgatgat gtaacacacc
     9601 gacggcactt ccaccatgat acccagagta atcgtctctg catgacgcag gccatcgcgc
     9661 ttaagctcaa cgatcgcttt ttggatctcg cccttcaccc ataagatctg atcgaggctg
     9721 tgaaccatcg ggatcatcaa ctgggcgttg ccaaaactgg cagcgcgcaa aatcgcccgt
     9781 agttgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg
     9841 aacgggttct cttcctgggg aatattcaga taaggaatgc ttttatcgcc tccgatgtcc
     9901 attgtgcgga agataatcgg cttgtcgccc gctgccagta gcacctgctg gtaggcttca
     9961 aattgctcct gctcgtccgg cgcgctgtcg cgatccatat agagcatttc agtacggaac
    10021 agtccaacgc cttccgcgcc gttggcaaac gcgcctggcg cttccagagc ggtgccgata
    10081 ttcgccgcaa tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcggcctgc
    10141 gcctgttgtt tttggcgttt atccgccagc gtctgtgcga cctgataata accgctcacc
    10201 gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt caagcaccgc cggttgcccg
    10261 gcataacggg caatcgcatc cagcggcagg ccgctcagta ccgggatcgc cgaggcgcgg
    10321 gccagaatca gggtatgcga ggtgcgccca gttttctcca gaatcatgcc tgcaagattt
    10381 tttaaatcga ggctcaaaaa ctggcttggg gttaaatcct cggctaccag aatagtcggt
    10441 ttttccagca ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt
    10501 tcgctaatat cgcgaatgtc gctaacacgt tcgcgcagat aatcactggc agaagcagaa
    10561 agtttggcgc aaacctgctc catattgctg atgatcgccg cccccagccc ttgatgctgt
    10621 tctgtcatca gacgacggat attgcccgcg aattcatcat cctgaatcag cgacaaatgg
    10681 gcgctgagga tagttttgct ttcgccgtca cgctcacgca attgctggtt cagttgctcg
    10741 gcaagcgttg ccaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc
    10801 cgataactgt cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt
    10861 gccagcacat tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga
    10921 tgcgccgtca gttctgcctg cgtcggctga acgctgtcgc tgtcgataaa gcgcaccagg
    10981 atgtactctt ccagcacgcg ccgcacctgc tcttcatcgc tgccgctaat gttcaggctg
    11041 caactgtcat taaataaggt gccagttcca atcaacgcca gcgagctttt agcatcggcc
    11101 tttgcgttct ggcgatggtt gaaaaaggtg atttcgcttt gccactggct gcactgttct
    11161 ttaagttccc acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc
    11221 gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc
    11281 gtctcacgcg taagacgttg aataagggca gataaaagtt gctccatctg caaaatgtcg
    11341 cggcaatcgg caatcgatgc cgcgcaatgt ccgtggcggg tggcaggccc cattaccact
    11401 gtgggtacgc cagtaccggt taagtgcacc gctccgccgt ccgtgccgcc gttgctgaac
    11461 atatctgcct gcaacggtac gccaatttca gccgccacgg tttcgatcca ggcggtaagt
    11521 tttggcggcg caatcagtga cttgtcgctt aacaccagca tcgggccgtt accaatctgg
    11581 cgatggttag ccgcgccata atcaaagttt ttcgcccagc aggcggtatc cagcacaatg
    11641 gcgacgtccg gcgacaccgc gcgggtagcg gtttgcccac cgcgtaatcc cacctcttcg
    11701 ctggaacttg ccaccagcca cacctccgca ggcagttcgg catcgtgcag ctcacgtagt
    11761 aacgtcacca gcagatagca accgaggcga tcatcaaagg ctttccccat cacccgctgg
    11821 tgagggagaa cctgaaaagt tgtatcaaag gtgacgcgat cgcctggacg aattcccgcc
    11881 tgcatcactt cgtcatacga gcgcgcacca atgtccacgc gcatggcgct gacgtcattc
    11941 ccctgccggt cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc
    12001 accggctgca gctggcgggc agccatgcgc acgttgccaa ccggcagcac atcaattgcc
    12061 ccttcgcggg agatgctacg caccataaat cccacttcgt ccatatgggc gcagatcatc
    12121 accttcggac ctgtcgattc attgaggcgg atcagcaccg atcccagacc atcaaatcgc
    12181 acttcttttt gcaggcgatc cgcttcttcc agcaggatct gccgcacttc ctgttccgag
    12241 gaggcgatcg catctgcctc gctcaacgct tttaatagcg ataaatccat tatgcctctc
    12301 ccgtgagcaa cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtaacca
    12361 gcacaacatc ttcgatgcgc acgccccctt gccctggcaa ataaatcccc ggctccacgg
    12421 tcagtaacat gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat
    12481 gaacttcaat gccgatagca tgaccggtgt tatgaccgaa atagtggcta aaacctgcct
    12541 cggtaatcac ttggcgcgcg gcatcgtcaa cctgctggca gcgcacgccg gggcgaattg
    12601 cagagattgc cgcgagctgt gcctgcagga caatctgata gacgtcaaac agcgggtgag
    12661 attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc
    12721 cctgatacag cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc
    12781 tggctttgcc gtgcggcagc gccccgcgcc agccgctggc gacaatggtg tcaaaagagg
    12841 ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc
    12901 tcatccccgc ctgaataaag cggcgaatat gctccgctcc gcgatcggca atcccacagg
    12961 cgaggcggat tatctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg
    13021 cgctaaccag tttcgcattg agttcagact tccagcggtg cgcggtttcc caactcacct
    13081 gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttga ttgacgatag
    13141 tggtaagcgt gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca cgggcttcca
    13201 cctcaacgta atagcgcgaa tccaccagaa tgtgcgcact ttcacggcta ataaccacat
    13261 agcctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca
    13321 ctgcatccag ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat gtcatctgga
    13381 ttttccttct tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag
    13441 gaacaccacc atcagcgcag ttatgactgc ccccagcgcg atccccgcca tatagacgcc
    13501 caggttagtg accagcggcc atgcccagat agcggactcc gggaaccatt gcactgcgcc
    13561 cagccagaca gcggcggttg agccgacaat agcgccgacc atatacgacg gaatggcggt
    13621 gatggggctt tccagcgcaa acggaatcgc accctcactg atccccataa acgccaggaa
    13681 catggcggtc ttaccctgcg gataaagttg agcgttgaac aggcgtttgc cggttaaacg
    13741 acggtcgata atggtcgcca gccccagacc aatcggcgga ataacgatag cgatagaacg
    13801 cgcggtaacc ggtaatacgt ggtcggtggt aaagctgaag gcaacgaaac ctgccgcttt
    13861 gttgattggg ccaccaaggt cgattgcggt cgccgcggca atccccattg catacatcag
    13921 cgcgcccttc tcacctgcgg cagtcagcac ggtacggata ccgccgttga tccagccacc
    13981 aaatggggtg atgacatagt acatcgccag catgacaaaa atggcggaaa gaatcggtag
    14041 caaaaatgtg gttttgaacg ctaacaggaa atctggtaac tggattttct ggttcatcca
    14101 tttcacaagg taaccagcaa cgatagatat aatcagcgca ccaatgaagg tggacggcac
    14161 cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg
    14221 ggtagacatc aagccgccga taaaaccagc cgggaacgcc agtttgccgc cgatagagtt
    14281 cgccacaaaa gcggcaaaca tcggaatagc aaagccaaac aacacgccgc cgaaggactg
    14341 cgacagccag gcaaatttca gtaaggagag gtcgaaaccg gagaatttcc cgctattaag
    14401 agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa tcagctggct
    14461 gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat
    14521 cacatgctgc gggagttcac cccagaagct gcttttagag gcctgcggat tcttaactgc
    14581 cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac
    14641 aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat
    14701 gatgcctaca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc
    14761 gaaacgttcg ttatcttccg gggtaactgc cacggaatgg atgatgatgg tcgcttcggc
    14821 gatatcctgc gccgtcaggc gattctggat gccgtccgcg ccctgggttt caattttcac
    14881 ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa aagtgtgagc
    14941 caggcccatc gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcgtt
    15001 cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa
    15061 taatgcattt actggagaat taatcccgct gtatggaagt gtgacgcagg tcggccttgt
    15121 gaggggacac aaagaaaaat tgaattaact gtatgattta aaagattatc gggagagtta
    15181 cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca
    15241 ccggaaccga gaagacccgg attgtcatgg acgatgagat acaccggaat atcatggaca
    15301 tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccagaggc tttgaagaac
    15361 tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg
    15421 agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcgcggcgg
    15481 caatcggtgc agctatcagc cagcgcacgt tcggtaatat cttttggctt gagattttct
    15541 ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa
    15601 agcacgcgtt ccgccgaaac atggccaatt tccgcacgca gtatttcgag gataatggcc
    15661 tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa gcttacccaa
    15721 cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc gtaaaccgca
    15781 ataggcttac cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc
    15841 atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg
    15901 agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag
    15961 tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct
    16021 tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta
    16081 gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc
    16141 acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat
    16201 tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct
    16261 gtaaatatcg atctgggtca cacatttact ttatcgtttc agcaccaatt gcagcgatgc
    16321 ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca
    16381 tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc
    16441 cagtcttatt ctgattgtgg tgatgaaaat cgcctcggca ttacagaatt tgctctggca
    16501 acgactgccg ggaactctgg ggatagccca ggattcacct ctctggatca tcggtgtatt
    16561 aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc
    16621 agaccccgcc tgtgaaccgc tgatcggcgc accagttccg ccctctgcgc taccaggact
    16681 tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat
    16741 catgaccgtc aatatcgccc ttgcggttgc cattggcgct cgtctgttac cgcgcgtcaa
    16801 ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc
    16861 tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg
    16921 ggatcgtctt tttgcgccat taatggcggc agcagctggt gcacttacta ccggattatt
    16981 tttccatccc catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat
    17041 tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg
    17101 cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat ccggtgctcg tgctgggtat
    17161 tggcggattt attctcggta ttctgggggt tattggtgga ccagtttcgc tgtttaaagg
    17221 gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt
    17281 gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg
    17341 gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt
    17401 acccgccgta ccagcggcaa taaccgtttc ttgcgctatt ctcggcatcg tgctggtggt
    17461 aacacgcgat ggctggttaa gtctttttat ggcggcggtc gttgtaccca ataccacatt
    17521 gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat
    17581 gatgatggtc aatcgtccga agcaacagcc accccacgat aacgtttagc aaaaaatgct
    17641 ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc
    17701 gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt
    17761 tctggcggcc gccctgatgg catttacccc gcttgcagca aacgcaggtg aaatcaccct
    17821 actgccatca atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg
    17881 tggtcactgg cgcgaccgtg actactggca tcgcaattat gagtggcgta aaaaccgctg
    17941 gtggcgtcat gataatggct atcatcgtgg ctgggataag cgtaaagcgt atgagcgtgg
    18001 ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatggaca
    18061 ccgccattaa acacattcgc caatggaaca caatgcctga tgcgacgcta gcgcgtctta
    18121 tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac gccgcatccg
    18181 ccaatagtgc cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaacaa
    18241 ccagatattc agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt
    18301 gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag
    18361 cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc
    18421 taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac gcacccacag
    18481 cgggatatgg aagcgaatga atccctgcat caccacctgc cccgccagtg tccccaccac
    18541 cgttgaggac agtccggcag caaccagact taacccaaag accgttgccg cagcatggct
    18601 taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga
    18661 aaagtggaac gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatcgt
    18721 catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg
    18781 taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa
    18841 cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt
    18901 acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac
    18961 aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat
    19021 taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag
    19081 aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat
    19141 aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat cacgaatctg
    19201 ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat
    19261 cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc tagcacccgc
    19321 ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg
    19381 tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata
    19441 gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga
    19501 gaatgattat caaattcatt tgaatgggtt gtggtgattt ctttgataga ccaggattat
    19561 gactacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca
    19621 taactttcag cttccataca caacatagca gaaatgtatg acagatcact atttttgaag
    19681 cttgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat
    19741 agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc
    19801 attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca
    19861 aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt
    19921 tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa
    19981 aactgctcgg atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag
    20041 gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg
    20101 gtattctcca gcacattcgc gtgttgccgg ttattatccg cgcaattggt ttcctgctct
    20161 ccaaagtcaa cggtatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg
    20221 gtcagtctga aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta
    20281 tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt ggtgcataca
    20341 tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt actgaacatg ttcagcacct
    20401 ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc cagtgaagaa aacatccaga
    20461 tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag
    20521 gtttcaaagt tgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg
    20581 cgctgaacgc actgtttgcc accgttactg gctggtttgg ctacagcatc tccttccagg
    20641 gcatcctggg ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag
    20701 cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga
    20761 tggatctgca gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc atctctgtgt
    20821 tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc
    20881 tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt tacggctcta
    20941 ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac catacataaa
    21001 aaagccgggg atcattcccc ggctttttta cgcctgttaa tcagctaatg gctgcgggcg
    21061 accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg cctgctgctg
    21121 ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat
    21181 tgatctcaca atcatcgcat ccagcgtatt cgtgacaata tctttcacaa agacgccatc
    21241 aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat atccggtgcc
    21301 aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct
    21361 gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg cctgcgggga
    21421 gatgtgataa cgcttaaaca gacgaataat ccgcccggca atattctttt gcagcagcgt
    21481 gagcggcatt aaattgactg aaaagcgcgg tccttttttg tcgcaagggt gtgttgccag
    21541 ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc
    21601 aataaggggc agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag
    21661 gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc
    21721 accctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc
    21781 ggaaaccatc tcctctctgc tatccagcgc cagcacatga tgatgtgcgc aggattgctc
    21841 cgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg
    21901 cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact
    21961 attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc tcagcactaa
    22021 cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca acggcagcag
    22081 cgtacggcaa attgagcgga tacagtgaac gcgcatcatt aagccgtaat gacgactcat
    22141 aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccgcctctt gctccggcgc
    22201 ttgttccaac gcacgaaagt tgggtaggag cgttaacgga tctgtcagcg cctgcaaatg
    22261 ccagcggcga ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac
    22321 gctaaaggaa atcagcaccg caagaataaa tgccagcgaa tattcggttt ccaccccttg
    22381 caaaaagttc tggttgtaat tcagaaggca aagcgttgaa acagcccagg tgagatttaa
    22441 aaacggatag cgaagcttac cgaccccgag ggtaaagatg atgaagaaaa cgggtaccaa
    22501 gtaaccggca ataaagtcgt tttcataagg tgtgcacaac agaagtagca gcacgcttaa
    22561 cgctgccagc cagcttaagg taaatgcgcg tttctctttg cccaacgacg gagcgatatc
    22621 cctgcgccac aatatctgcg caaagtgggg acttacaatc atgcgagtga gatagtagaa
    22681 aagcatgttg taaatcagca cagcggtgaa aaggcttagc aaatcaacga ccgtgaaaat
    22741 ggcatccgca tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac
    22801 aagatacatg ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg
    22861 ccagacatat cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat
    22921 tgcgcaggca cacaacacga caaacgtctg gctaccgagc atgaccgcag tctgtaaaaa
    22981 agagagatga aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc
    23041 gcgccagcca aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa
    23101 gatataactg gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac
    23161 cacggtgagc gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac
    23221 aaacatgagt acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat
    23281 tcgcatcgcg aagcaaacag gggccagcct gttgccgatt tttcatccaa tgatgctttc
    23341 accaggcggc gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa
    23401 aaaacccccg ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc
    23461 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt
    23521 acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc
    23581 tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt
    23641 cgagttgacg ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcaacg
    23701 tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc
    23761 agtagttatt catgtcacgg tttcctgtaa agtggtgtta taaaatgaac tactaataga
    23821 cccacataca ttcagggaat tgttatgttc aaggagagga tgacaccaga tgaacttgcc
    23881 agactgaccg gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggaca
    23941 acgtcaccaa aacctggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag
    24001 gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc agggagaagc gcctgccctt
    24061 tctggtgatg cgccgcttga agtattgctg gtgacactgg cgaaagagat gacgccagtt
    24121 gaacaaaaac agtttacatc cttgcttctg cgggaaggga ttatcggatt gttacaacgc
    24181 ttagggattc gcgatagcaa ataatatgaa aagattacgc aacaaaatga ccaccgaaga
    24241 actggctgaa tgcctcggtg tggctaaaca aactgttaac cgttggatca gagaaaaagg
    24301 ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta ttctggtcga
    24361 tacgcaagtt tgcgagttta ttcagaacac gccagccttc cataacacac caatgttgat
    24421 ggaagctgaa gagagaattg ctgaatacgc tcctggagca cgcgccccgg cgtatcgaca
    24481 aatcatcaac gcgatagata atatgacaga cattgagcag gaaaaagtcg cgcaattttt
    24541 gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat attgacgaat cagcataaat
    24601 aaaaaacggc aggatattat ctcctgccgt ttatcttttt acacgctaat tactgctgat
    24661 tttcgcgttc agcaataaaa tccagcgctt tgttgatacg ctcgatactg cgggtcttac
    24721 caattgcgtg aacggtaaca tccagtgctg gagactgccc cgcaccggtt acggcgacac
    24781 gcagcggcat accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag
    24841 cgtgatgaac gttttcagcg gtccagtcag taatcgcggc cagtttgtca cgaaccactt
    24901 ccagcggctg acgcgctacc ggacgcagat gttttttcgc cgcatcggca tcgaactcag
    24961 caaaatcttc gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt
    25021 cgcccagcag tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct
    25081 gctcaatgtg ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt
    25141 gatggttcag ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat
    25201 tcaaagtgaa gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc
    25261 agcccagacg caccagatag ttcagcagtg cttctggcaa ataaccgtca tcacgatact
    25321 gcattacgct gactgccccg tgacgtttgg acagtttttt accgtcatcg ccattgatca
    25381 tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc cttaagaatg ttgatctggc
    25441 gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt
    25501 catcgacaac cacacagaag ttataggttg gggaaccatc ggtacggcgg ataatcagat
    25561 catccagctc ctggttgctg aactcgatcg gaccacggat ctgatcgtca aaaacaacag
    25621 aaccttcctg cggattagca aaacgcacga cacacggttc atcatcagca tgatgctcat
    25681 ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc
    25741 gcagcgcttc caggcgctct ttagagcaat agcatttata agcagtgccc tcttccagca
    25801 tctgatcgat caccgcattg tagcgatcaa aacgtttggt ctggtagtac ggaccttcat
    25861 cccactccag gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg
    25921 aacgctcaag atcggtgtct tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa
    25981 aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg
    26041 gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc
    26101 ggcaaatcct ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg
    26161 tagttcgggt ttctacctta cgcattattg ttttcgttga caaattgcgc aacacggtct
    26221 gttttgcgat catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt
    26281 gactcatttt gaactctccc tataatgcga atccacacag cgggggtgat tagctcagct
    26341 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa
    26401 ctactttatg tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct
    26461 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac
    26521 ttcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
    26581 aatcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
    26641 atactcaaag attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg
    26701 ctcgcggcga cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgta
    26761 aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gcgaaattgc ctgatgcgct
    26821 tcacttagca gacgactatt tccggcaatt cctgtctcct cacctactgt gtcaatgcag
    26881 ccaacagctt aaccatcgcg ggcgtcacct gctgtgtttc ataaacaata tataaatctg
    26941 cagggatgcg ctgtttgagc ggacggaaaa tgacacctgg ccagttcatt tgtgcgtagc
    27001 tgtccgctat caatgtgata ccaataccca tactgaccat ggcgagtacc gtttgcggtt
    27061 cattaacttc gcgaataaca accggtgaaa atcccacctg ctggcaaact cgctgcaaaa
    27121 aatcccagtc agtgtacacg ggcggcattg taacaaaata ctcgtcacgt agcgcttcca
    27181 gcggaacggt ggaaaatgat gagagatgat gctcttcagg catcgccacc agaaacgccg
    27241 attcatgcaa ccgtaagctg gtaaaacctg tcggtggttc tgtcgccatt cgccagatcc
    27301 cggcatcgag ttcacgtcgt tccagcaggg ccatttgcat cgcgggcatc ttttcgcgaa
    27361 aaagaacttc aacgttagga ttttccctga ggaatcgccg cataaccggg cgcatccgtc
    27421 cccacatagc cgttcccact acgccgagtt caatccgccc tgcttctccc cgacctattt
    27481 gttcaacccg agccaataca ttattagcat tcaccagcaa tcgacgcgat tcttccatca
    27541 agattttgcc cgcgtgtgtc agtacgacgc tgcgcgaatg gcgaataaaa agctgcgtgc
    27601 cgagttgatt ttccagctct ttaatatgaa tactgagcgg aggctgagac atatttaaac
    27661 gcgctgctgc gcggccaaaa tgcaactctt ccgctacggc aagaaaataa cggagcaact
    27721 taagatctgt tctgtatacg cgttccataa ttaag
//
LOCUS       DAPHDX010000056        27455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPHDX010000056 DAPHDX010000000
VERSION     DAPHDX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.056"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(42..1292)
                     /gene="icd"
                     /locus_tag="RH058_002309"
     CDS             complement(42..1292)
                     /gene="icd"
                     /locus_tag="RH058_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV3137889.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1464..2117
                     /gene="rluE"
                     /locus_tag="RH058_002310"
     CDS             1464..2117
                     /gene="rluE"
                     /locus_tag="RH058_002310"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV3137890.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2127..2588
                     /gene="nudJ"
                     /locus_tag="RH058_002311"
     CDS             2127..2588
                     /gene="nudJ"
                     /locus_tag="RH058_002311"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV3137891.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2642..3748
                     /gene="mnmA"
                     /locus_tag="RH058_002312"
     CDS             2642..3748
                     /gene="mnmA"
                     /locus_tag="RH058_002312"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV3137892.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGIEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3784..4425
                     /gene="hflD"
                     /locus_tag="RH058_002313"
     CDS             3784..4425
                     /gene="hflD"
                     /locus_tag="RH058_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV3137893.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4429..5799
                     /gene="purB"
                     /locus_tag="RH058_002314"
     CDS             4429..5799
                     /gene="purB"
                     /locus_tag="RH058_002314"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV3137894.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5967..6638
                     /gene="phoP"
                     /locus_tag="RH058_002315"
     CDS             5967..6638
                     /gene="phoP"
                     /locus_tag="RH058_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV3137895.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6638..8098
                     /gene="phoQ"
                     /locus_tag="RH058_002316"
     CDS             6638..8098
                     /gene="phoQ"
                     /locus_tag="RH058_002316"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV3137896.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8174..9295
                     /gene="roxA"
                     /locus_tag="RH058_002317"
                     /gene_synonym="ycfD"
     CDS             8174..9295
                     /gene="roxA"
                     /locus_tag="RH058_002317"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV3137897.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9344..10570)
                     /gene="pepT"
                     /locus_tag="RH058_002318"
     CDS             complement(9344..10570)
                     /gene="pepT"
                     /locus_tag="RH058_002318"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV3137898.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10820..11956
                     /gene="potA"
                     /locus_tag="RH058_002319"
     CDS             10820..11956
                     /gene="potA"
                     /locus_tag="RH058_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV3137899.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11940..12797
                     /gene="potB"
                     /locus_tag="RH058_002320"
     CDS             11940..12797
                     /gene="potB"
                     /locus_tag="RH058_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV3137900.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12794..13588
                     /gene="potC"
                     /locus_tag="RH058_002321"
     CDS             12794..13588
                     /gene="potC"
                     /locus_tag="RH058_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV3137901.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13585..14631
                     /gene="potD"
                     /locus_tag="RH058_002322"
     CDS             13585..14631
                     /gene="potD"
                     /locus_tag="RH058_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV3137902.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14689..15150
                     /locus_tag="RH058_002323"
     CDS             14689..15150
                     /locus_tag="RH058_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDV3137903.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15147..15572
                     /locus_tag="RH058_002324"
     CDS             15147..15572
                     /locus_tag="RH058_002324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDV3137904.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWY"
     gene            15588..15935
                     /locus_tag="RH058_002325"
     CDS             15588..15935
                     /locus_tag="RH058_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3137905.1"
                     /translation="MAVLQAQILKQPTPPLTYYPHPGQQHENAPPGSTNTLGFIAWAA
                     TFILFSWIFCYTTRFIYALKFAVAMTIANMGYQALCLYIDNSFAITRITPLWAGLIGV
                     CTFIAALLLTSKR"
     gene            complement(16074..16895)
                     /gene="cobB"
                     /locus_tag="RH058_002326"
     CDS             complement(16074..16895)
                     /gene="cobB"
                     /locus_tag="RH058_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV3137906.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16911..17822)
                     /gene="nagK"
                     /locus_tag="RH058_002327"
     CDS             complement(16911..17822)
                     /gene="nagK"
                     /locus_tag="RH058_002327"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV3137907.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17851..19095)
                     /gene="lolE"
                     /locus_tag="RH058_002328"
     CDS             complement(17851..19095)
                     /gene="lolE"
                     /locus_tag="RH058_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV3137908.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19095..19796)
                     /gene="lolD"
                     /locus_tag="RH058_002329"
     CDS             complement(19095..19796)
                     /gene="lolD"
                     /locus_tag="RH058_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV3137909.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19789..20988)
                     /gene="lolC"
                     /locus_tag="RH058_002330"
     CDS             complement(19789..20988)
                     /gene="lolC"
                     /locus_tag="RH058_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV3137910.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21250..22323
                     /locus_tag="RH058_002331"
     CDS             21250..22323
                     /locus_tag="RH058_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3137911.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDIRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22451..25897
                     /gene="mfd"
                     /locus_tag="RH058_002332"
     CDS             22451..25897
                     /gene="mfd"
                     /locus_tag="RH058_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV3137912.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANTNLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26041..27003
                     /gene="ldtC"
                     /locus_tag="RH058_002333"
     CDS             26041..27003
                     /gene="ldtC"
                     /locus_tag="RH058_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV3137913.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRADSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27086..27343)
                     /gene="bhsA"
                     /locus_tag="RH058_002334"
     CDS             complement(27086..27343)
                     /gene="bhsA"
                     /locus_tag="RH058_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV3137914.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6690 a         7111 c         6949 g         6705 t
ORIGIN      
        1 gcgtgttata cgcccgttgc aatattaaac acatgtagtg attacatgtt cttgatgatc
       61 gcatcaccaa attctgaaca tttcagcagt ttagcgcctt ccatcagacg ctcgaagtca
      121 taggttacgg tcttcgcatt gattgcgcct tccatacctt taacaatcag gtctgcggct
      181 tcagtccagc ccatatggcg taacatcatc tcagcggaga gaataataga gccagggttt
      241 actttgtcct gaccggcgta tttcggcgca gtaccgtggg tggcttcaaa cagggcgcat
      301 tcgtcaccga tgtttgcacc aggggcgata ccgataccgc caacctgcgc tgccagggcg
      361 tcagaaatgt agtcaccgtt caggttcata caggcgataa catcatattc agccggacgc
      421 agcaggatct gttgcaggaa tgcatcagca atcacgtctt taatgacgat ctctttgccg
      481 gtgttcgggt ttttaacttt cagccacggg ccgccgtcga tcagttcacc gccaaactct
      541 tcacgcgcca gctggtagcc ccagtcttta aacgcgcctt cggtgaactt catgatgttg
      601 cctttgtgca ccagggtcac agagtcacgg tcgttggcaa ttgcgtattc aatcgcggca
      661 cggaccagac gtttggtgcc ttcttcagaa cacggcttga taccgatacc gcaatgttcc
      721 gggaagcgaa ttttcttcac gcccatctct tcacgcagga atttaatcac tttctcggcg
      781 tcggcagagt ctgctttcca ttcgataccc gcataaatgt cttccgagtt ttcacggaag
      841 ataaccatat cggtcagttc agggtgttta accgggcttg gagtgccttg atagtaacgt
      901 accggacgca ggcagatgta gagatccagt tcctggcgca gggcaacgtt cagagagcga
      961 ataccgccac caacaggagt ggtcagcgga cctttaatgg caacgcgata ttcacgaatc
     1021 agatcaaggg tttcagcagg cagccagaca tcctgaccat aaacctgtgt ggatttttca
     1081 ccggtgtaaa tttccatcca ggagatttta cgctcgcctt tataggcttt ctcgactgca
     1141 gcgtcgacca ctttcagcat ggctggggtt acatctacac cgattccatc accttcaatg
     1201 taagggataa tcggattttc aggaacgttg agtttgccgt tttgcagggt gatcttcttg
     1261 ccttgtgccg gaacaactac tttactttcc attcacctct ccttcgagcg ctactggttt
     1321 gctcgtctgc caccacgttg catatgcgtt tgcgtcctgc gatacggatg ctttagagca
     1381 attttttgtt aatgatttgt aattggcttg tcaatactac cctattgttt gccgtcatga
     1441 aagatgcgcg ttattaggct ataatgcggc aattcataat ctctgaaaat accatgcaaa
     1501 aaacttcttt tagaaatcac caggttaagc gattcagctc gcaacgttct accaggcgta
     1561 aacctgaaaa ccagcccacg cgtgtgatcc tgttcaataa accctacgat gttcttccgc
     1621 agttcaccga tgaagccgga cgcaaaacat taaaagaatt catcccggtt cagggcgttt
     1681 atgcagcagg tcgtcttgac cgcgatagcg aagggttact ggtgctgacc aataacggcg
     1741 cgttgcaggc gcgtttaacc cagccgggta aacgcaccgg gaaaatctat tatgtgcagg
     1801 tggaaggtat tcccacacaa gacgcacttg aagccttgcg caatggcgta accttaaatg
     1861 atggccctac cctgcccgcc ggcgcggagc tggttgacga accagcgtgg ttatggccgc
     1921 ggaatccacc aattcgtgaa cgcaaaagta ttcccaccag ctggctgaag atcaccttat
     1981 atgaaggacg taatcgccag gtgcgccgca tgaccgccca tgttggtttc cccacgctgc
     2041 gactgattcg ctatgcgatg ggagattact ctttggataa tcttgccaat ggcgaatggc
     2101 gagaagtgac agattaagga tgcaaaatgt ttaaaccgca cgttaccgtt gcttgcgtgg
     2161 tgcacgcaga aggcaaattt ttagtcgttg aagagacgat taatggtaaa gcgttatgga
     2221 accaacctgc cgggcatctg gaagccgatg aaaccttagt ggaagccgcc gcccgtgaac
     2281 tgtgggaaga aaccggcatc agcgcgcagc cgcaacactt tattcgtatg catcagtgga
     2341 ttgcgccaga taaaacgccg tttttgcgtt tcctctttgc cattgagctt gagcaaatat
     2401 gcccgaccca gcctcatgac agcgatatcg actgctgtcg ttgggtcagc gccgaagaga
     2461 ttttaaaggc gtcaaatctt cgttcgccgc tggtggcgga aagtatccgt tgttatcaaa
     2521 gcgggcaacg ctatccgctg gagatgattg gcgattttaa ctggcctttt acaaagggtg
     2581 tcatctaaaa ggctgcgtga tagaatacgc cgccttgaag ttcaatgtcg tgagtgatcc
     2641 aatgtctgaa accgcaaaaa aagtaatcgt cggcatgtcc ggcggtgtcg attcctccgt
     2701 ttctgcctgg ctgttgcaac aacagggata tcaggttgaa ggcctgttta tgaagaactg
     2761 ggaagaagac gacggtgagg aatattgcac agcggcagcg gatctggctg atgcccaggc
     2821 tgtctgcgac aagctcggca ttgaactgca caccgttaac tttgctgccg agtactggga
     2881 caacgtcttc gaactgttcc ttgccgaata taaagccggt cgcacgccga atccggatat
     2941 tctgtgcaac aaagagatca aatttaaagc cttcctcgaa tttgccgccg aagatttagg
     3001 tgccgattat atcgctaccg gtcattacgt acgtcgggcc gatgtcgatg gcaagagccg
     3061 cctgctgcgt ggtctggaca gcaataaaga ccagagctac ttcctttata cgctcagcca
     3121 tgagcagatt gcgcaaagcc tgttcccggt cggcgaactg gaaaaaccgc aggtgcgtaa
     3181 gattgctgaa gatcttggtc tggtcaccgc gaagaaaaaa gactctaccg gcatttgctt
     3241 cattggcgaa cgtaaattcc gcgagttcct gggccgttat ctcccggcgc aaccgggcaa
     3301 aatcattacc gtcgatggcg atgaaattgg tgagcaccag gggctgatgt atcacactct
     3361 cggtcagcgt aaaggtctgg gtatcggtgg caccaaagaa ggtatcgaag aaccgtggta
     3421 tgtggtggac aaagacgtcg aaaacaacat tctggttgtc gctcagggcc atgaacaccc
     3481 gcggctgatg tctgtcgggt tgattgccca gcagttgcac tgggtcgatc gcgaaccatt
     3541 caccggcact atgcgttgca cggtaaaaac ccgctatcgc cagaccgata tcccttgcac
     3601 cgtcaaggcg ctggacgatg atcgcattga agtgattttc gatgaaccgg ttgccgccgt
     3661 gacgccgggc cagtctgccg tcttctataa cggtgaagtg tgcctcggtg gcggtattat
     3721 tgagcagcgt ctgccgctgc cggtctgatt attatcttta cttaaaacaa ggaagcagtg
     3781 aacgtggcaa agaattacta tgacatcacc ctcgccctgg ccggtatttg tcagtcggca
     3841 cgcctggtgc aacaactcgc tcaccagggg cattgtgatg ccgatgcgct acacgtctca
     3901 ctcaacagta ttattgatat gaaccccagc tcgacgctgg cggtttttgg cggtagcgaa
     3961 gccaacctgc gcgtcgggct ggaaaccctg ctcggcgtgc tcaatgccag cagtcgccag
     4021 ggcttaaacg ccgaattaac ccgctacaca ctcagcttga tggtgcttga gcgcaaactc
     4081 tcctcagcga aaggcgcgct cgacactctg ggcaaccgga tcaacggcct gcaacgccag
     4141 ctcgaacact tcgatttaca gtccgaaacg ctgatgagcg cgatggctgc tatctatgtt
     4201 gatgtgatca gcccgcttgg cccgcgcatt caggtcaccg gttcccctgc tgtactgcaa
     4261 agcccacaag tgcaggcgaa agttcgcgca accctgctgg caggcattcg cgccgccgtg
     4321 ctctggcacc aggtcggcgg cggacgtctg caactgatgt tttctcgtaa tcgcctgacc
     4381 actcaggcaa aacaaattct tgctcattta accccggagt tgtgatctat ggaattatcc
     4441 tcactgaccg ccgtttcccc tgtcgatgga cgctacggcg ataaagtcag cgcgctgcgc
     4501 gggattttca gcgaatatgg tttgctgaaa ttccgtgtac aagttgaagt acgttggctg
     4561 caaaaactgg ccgcgcacgc agcgatcaag gaagttcctg cttttgctgc cgacgcaatc
     4621 ggttaccttg atgcaattgt cgccaatttc agcgaagaag atgccgcacg catcaaaacc
     4681 atcgagcgta ccactaacca cgacgttaaa gcggttgagt atttcctgaa agaaaaagtg
     4741 gcggagatcc cggaactgca cgcggtttct gaattcatcc actttgcctg tacttcggaa
     4801 gatatcaata acctctccca cgcattaatg ctgaaaaccg cgcgtgatga agtgatcctg
     4861 ccgtactggc gtcaactgat tgatggcatt aaagatctcg ccgttcagta ccgcgatatc
     4921 ccgctgctgt ctcgtaccca cggtcagcca gccacgccgt caaccatcgg taaagagatg
     4981 gcaaacgtcg cctaccgtat ggagcgccag taccgccagc ttaatcaggt ggagatcctc
     5041 ggcaaaatca acggcgcggt cggtaactat aacgcccata tcgccgctta cccggaagtt
     5101 gactggcatc agttcagcga agagttcgtc acctcgctgg gtattcagtg gaacccgtac
     5161 accacccaga tcgaaccgca cgactacatt gccgaattgt ttgattgcgt tgcgcgcttc
     5221 aacaccattc tgatcgactt tgaccgtgac gtctggggtt atatcgccct taaccacttc
     5281 aaacagaaaa ccattgctgg tgagattggt tcttccacca tgccgcataa agttaacccg
     5341 attgacttcg aaaactccga agggaatctg ggcctttcca acgcggtatt gcagcatctg
     5401 gcaagcaaac tgccggtttc ccgctggcag cgtgacctga ccgactccac cgtgctgcgt
     5461 aacctcggcg tgggtatcgg ttatgcgctg attgcgtatc aatccaccct gaaaggcgtg
     5521 agcaaactgg aagtaaaccg tgaccatctg ctggatgagc tggatcacaa ctgggaagtg
     5581 ctggctgagc caatccagac agttatgcgt cgctatggca tcgaaaaacc gtacgagaag
     5641 ctgaaagagc tgactcgcgg taagcgcgtt gacgccgaag gcatgaagca gtttatcgat
     5701 ggtctggcgt tgccagaaga agagaaagcc cgcctgaaag cgatgacgcc ggctaactat
     5761 attggtcgag ctatcaccat ggttgatgag ctgaaataaa ctcgtatcag tgccggatgg
     5821 cgatgctgtc cggcctgttt attaaaatta tccacttttt cttttttcac tttacctccc
     5881 ctccccgctg gtttatttaa tgtttacccc cataaacaca taatcgcgtt acactatttt
     5941 aataattaag acagggagaa ataaaaatgc gcgtactggt tgttgaagac aatgcgttgt
     6001 tacgtcacca ccttaaagtt cagattcagg atgctggtca tcaggtcgat gacgcagaag
     6061 atgccaaaga agccgattat tatctcaatg aacatttacc ggatattgcg attgtcgatc
     6121 tcggattgcc tgacgaggac ggtctgtcac tgattcgccg ctggcgtagc aatgatgttt
     6181 cactgccgat tctggtatta accgcccgtg aaagctggca ggacaaagtc gaagtattaa
     6241 gtgccggtgc tgatgattac gtgactaaac cgtttcatat tgaagaggtg atggcgcgaa
     6301 tgcaggcatt aatgcggcgt aatagcggcc tggcttcaca ggtcatttcg ctacccccgt
     6361 ttcaggttga tctctctcgc cgtgaattat ctattaatga cgaagtgatc aaactgaccg
     6421 cgttcgaata caccattatg gaaacgttga tacgcaataa tggcaaagtg gtgagcaaag
     6481 attcgttaat gctccaactc tatccggatg cggagctgcg ggaaagccat accattgatg
     6541 tactgatggg acgtctgcgc aaaaaaattc aggcacaata tccgcaagaa gtgattacta
     6601 ccgttcgcgg ccagggctat ttgttcgaat tgcgctgatg aaaaaattac tgcgtctttt
     6661 tttcccgctc tcactgcggg tacgttttct gttggcaacg gctgcggtag tactggtgct
     6721 ttcgcttgcc tacggaatgg tcgcgctgat cggttatagc gtcagtttcg ataaaactac
     6781 gtttcggctg ttacgtggcg agagcaatct gttctatacc cttgcgaagt gggaaaacaa
     6841 taagttgcat gtcgagttac ccgaaaatat cgacaagcaa agccccacca tgacgctaat
     6901 ttatgatgag aacgggcagc ttttatgggc gcaacgtgac gtgccctggc tgatgaagat
     6961 gatccagcct gactggctga aatcgaatgg ttttcatgaa attgaagcgg atgttaacga
     7021 taccagcctc ttgctgagtg gagatcattc gatacagcaa cagttgcagg aagtgcggga
     7081 agatgatgac gacgcggaga tgacccactc ggtggcagta aacgtctacc cggcaacatc
     7141 gcggatgcca aaattaacct ttgtggtggt ggataccatt ccggtggagc taaaaagttc
     7201 ctatatggtc tggagctggt ttatctatgt gctctcagcc aatctgctgt tagtgatccc
     7261 gctgctgtgg gtcgccgcct ggtggagttt acgccccatc gaagccctgg caaaagaagt
     7321 ccgcgaactg gaagaacata accgcgaatt gctcaatcca gccacaacgc gagaactgac
     7381 cagtctggta cgaaacctga accgattgtt aaaaagtgaa cgcgaacgtt acgacaaata
     7441 ccgtacgacg ctcaccgacc tgacccatag tctgaaaacg ccactggcgg tgctgcaaag
     7501 tacgctgcgt tctctgcgta gtgaaaagat gagcgtcagt gatgctgagc cggtaatgct
     7561 ggagcaaatc agccgcattt cacagcaaat tggctactac ctgcatcgtg ccagtatgcg
     7621 cggcgggaca ttgctcagcc gcgagctgca tccggtcgcc ccactgctgg acaatctcac
     7681 ctcagcgctg aacaaagtgt atcaacgcaa aggggtcaat atctctctcg atatttcgcc
     7741 agagatcagc tttgtcggtg agcagaacga ttttgtcgag gtgatgggca atgtgctgga
     7801 taatgcctgt aaatattgcc tcgagtttgt cgaaatttct gcaaggcaaa ccgacgagca
     7861 tctctatatt gtggtcgagg atgatggacc cggtattcca ttaagcaagc gagaggtcat
     7921 tttcgaccgt ggtcaacggg ttgatacttt acgccctggg caaggtgtag ggctggcggt
     7981 agcccgcgaa atcaccgagc aatatgaggg taaaatcgtc gccggagaga gcatgctggg
     8041 cggtgcgcgg atggaggtga tttttggtcg ccagcattct gcgccgaaag atgaataaat
     8101 atgtccatac ttcacgcatt acgttaagca tccgttataa tcggttgcag ataccagcct
     8161 gtggatgctt aacatggaat accaactcac tcttaactgg cccgattttc ttgaacgtca
     8221 ctggcagaaa cgcccggtgg tgttaaaacg cggctttaat aattttattg acccgatctc
     8281 tccagacgag ttggcgggtc tggcgatgga aagcgaagtc gacagtcgac tggtcagtca
     8341 ccaggatggc aaatggcagg tcagccacgg tccgttcgaa agctacgatc atctcggtga
     8401 aactaactgg tcattgttag tgcaagcagt aaatcactgg catgagccga ccgccgcgct
     8461 gatgcgaccg ttccgtgaac taccggactg gcgtattgat gatctgatga tctctttttc
     8521 tgtacctggc ggcggcgtcg gcccgcatct cgatcagtac gacgtgttta tcattcaggg
     8581 taccggacgc cgtcgctggc gagtgggcga aaagctgcaa atgaaacagc actgcccaca
     8641 cccggatctg ttacaggtcg atccgttcga agccatcatc gatgaagagc tggagcctgg
     8701 cgatattctt tatattccgc caggattccc gcatgaaggc tacgcgctgg aaaatgcgat
     8761 gaactattcc gtgggttttc gcgcgccaaa tacgcgggaa ttaattagcg gatttgccga
     8821 ttatgtgctg caacgtgaac tgggcggcaa ctactacagc gatccggatg ttccacctcg
     8881 cgctcatcct gcggacgttc tgccgcaaga aatggataaa ctgcgtgaga tgatgctcga
     8941 attgatcaac cagccggaac actttaagca atggtttggc gagtttatat cccagtcacg
     9001 tcatgaactg gatatcgcgc cgccggaacc gccttatcag ccagatgaaa tctacgatgc
     9061 gctgaaacaa ggtgatgtgc tggtgcgcct gggtggtctg cgagtattgc gcattggcga
     9121 cgacgtgtat gccaatggtg agaagatcga ttccccgcac cgtccggcac tggatgcact
     9181 cgccagcaac attgcgctga ccgcggagaa ttttggcgat gcgctggaag atccgtcatt
     9241 cctcgcgatg ctcgcggcgc tggtcaatag cggatactgg ttcttcgaag ggtaagtttg
     9301 ggtttaagcc ggatgcggca tcacatgccg catccctttt cgcttacttc cgttgcgccg
     9361 ttaactcggc aatacggacg atcacctgca ccgctttttc cataccttcc agagtcacaa
     9421 actcatgctt accatgatag ttgtaaccgc cagtgaacag gttcgggcac ggtaatccca
     9481 taaacgacaa ctgcgcgccg tcggtaccgc cgcggatcgg tttcagttcc ggttcaatat
     9541 cgcagtcacg catcgcctgc tgggcgatat cgagaatatg cggatgctca accactttct
     9601 cgcgcatatt gtagtaactg tcttcaatca ccagttcaat gtagcaatca ggatgtaacc
     9661 ctttgcccac ttttttggcg atctccatca ttttacgttt acgcgcttca aactgtttac
     9721 ggtcgaaatc acggatgatg tagtgcatat cggctcggtc aacggtgcct ttcatgctcg
     9781 ccaggtgata gaaaccttca tagccttctg tcatttccgg gctttcatcc gccggaactt
     9841 ccgcatgaat acgtgccgcc agcgacagcg catttaccat cactcctttc gccgtgcccg
     9901 gatgaacatt gttaccgaca attttgatat tgaccgatgc ggcgttgaag ttttcaaact
     9961 ccagttcgcc tacgccacca ccgtcaacag tgtaagccca gcgggcatcg aaggcatcaa
    10021 catcaaaatg tttcgcccct ttgcccactt cttcatccgg ggtaaaggcg acgcgaatat
    10081 caccatgcgg aatgtttttc tgttgcaata ccgccagcgc ggtcatgatt tctgcaatac
    10141 cggctttgtc atcggcacct aacaaggttt taccatcggt ggtaatcaac gtctgaccca
    10201 gtagctgatg cagcaccggg aacataaccg gtgataaaac ttcatcgccg atacccagcg
    10261 caatatcgcc accgcgatag ctttcaacaa tttgcggatt aacatttttg ccgctgcaat
    10321 ccggtgaggt atccacatga gaaataaagc caatcgccgg gatatcgcca gggacgttag
    10381 ccggtaacgt cgccatcaaa gtgcccttct cacttaaggt cacattgata agccccattt
    10441 cttcgagctg ctctttcagc agatgcaata acttccattg gccttccgtg ctgggaacct
    10501 gtctcacccc tgcttttgat tgggtatcca gagacacgta gttcaaaaat cgctcaagta
    10561 gtttatccat gtagtcaccc tcactttttg tgacaacatt attaagaagc aagaaaagac
    10621 aaatattgcg tcaggtcact tttacccctg caagcgggaa tatttatcag cattacctta
    10681 atgaatataa agctaagcca gtaattcaca acaaagattg gcgattcaag cggtttgccg
    10741 tagaattacc gccctcatta agagtcacca aggtggttaa ccacaaaccc cgcatcggta
    10801 agccatccgt tgcgtttaca tgggacagag taaaaaattg aataaacaac cgagttcgct
    10861 ttcaccgctg gtgcaattgg cgggaattcg caaatgcttt gatggtaaag aggtcattcc
    10921 ccagctggat ctgactatca acaatggcga gttcctcacg ctgcttggcc cttctggctg
    10981 cggtaaaaca accgttcttc gcctgattgc aggtctggaa actgttgatt ccggacgcat
    11041 catgctggat aacgaggaca tcacccacgt tccggcggaa aaccgctatg tgaacactgt
    11101 tttccaaagc tacgcacttt tcccccacat gaccgtgttc gaaaatgtgg cctttgggtt
    11161 gcgcatgcaa aaaacccccg ctgctgaaat tacgccccgc gtgatggaag ccctgcggat
    11221 ggtgcaactg gaaaccttcg ctcaacgtaa accgcatcag ctctctggtg gtcaacagca
    11281 acgcgtcgcc attgctcgcg cggtggttaa caagcctcgt ctgttgttgc tggatgagtc
    11341 gctctcagcg ctggattaca aactgcgtaa gcaaatgcag aacgagctga aagcgttaca
    11401 gcgtaagctt ggtattacct tcgtctttgt cactcacgat caggaagaag cgctcaccat
    11461 gtcagacagg attgtggtga tgcgcgaggg tcgtattgag caagatggta caccgcgcga
    11521 aatttacgaa gagccgaaaa acctgtttgt tgccggcttc attggcgaaa tcaatatgtt
    11581 taacgccact gtcatcgaac gtctagacga gcagcgcgta cgcgccaacg ttgaaggccg
    11641 cgaatgtaat atctacgtta acttcgccgt tgaaccgggg caaaaactgc atgttctgct
    11701 gcgcccggaa gacttacgtg ttgaagagat taacgacgac aaccacgctg aagggctgat
    11761 tggttacgtt cgtgagcgta actacaaagg catgacgctg gagtcggttg ttgaactgga
    11821 aaatggcaag atggtgatgg tcagcgaatt cttcaatgaa gacgaccctg actttgacca
    11881 ctctctcgac caaaaaatgg ccattaattg ggtagaaagc tgggaggtcg tactggctga
    11941 tgaagaacac aagtaagttc cagaatgtag tgattgtcac tattgtcggt tggcttgtgt
    12001 tgtttgtctt tctgcccaac ctgatgatca ttggcaccag ctttttgacc cgcgacgacg
    12061 ccagtttcgt caaaatggtc tttacgctgg ataactacac gcgtctgctc gatccgctct
    12121 attttgaagt gctattgcac tcgctgaata tggcgctgat cgccaccctc gcctgcctgg
    12181 tgctgggcta cccgtttgcc tggtttctgg cgaagctacc acacaaggtg cgtccgctgc
    12241 tgctgtttct gctgattgtt cccttctgga ccaactcact gattcgcatc tacgggctga
    12301 aaattttcct cagcaccaaa ggctatctca acgagttttt gctctggctg ggcgttatcg
    12361 acacaccaat tcgtatcatg ttcacgccca gtgcggtgat tatcggtctg gtttacattc
    12421 tgctaccgtt tatggtgatg ccgctgtact ccagtatcga aaaactggat aagccgttgc
    12481 ttgaggcggc gcgcgatctc ggtgccagca agttacagac ttttatccgt atcattattc
    12541 cactgacgat gccgggaatt attgccggat gtctgctggt gatgctgcca gcgatgggcc
    12601 tgttctatgt atccgacctg atgggcggtg cgaaaaacct gctgatcggt aacgtcatca
    12661 aggtccagtt cctcaatatt cgtgactggc cgtttggtgc agccaccagc attacgctga
    12721 ctatcgtaat gggcctgatg ttgctggttt actggcgcgc ttctcgtctg ctgaataaaa
    12781 aggtggaact cgaatgatcg gtcgactgct tcgcggcggt tttatgaccg ctatctacgc
    12841 gtacctgtat atcccaatca ttattttgat tgtgaactcc tttaacagct cgcgctttgg
    12901 catcaactgg cagggtttta ccaccaaatg gtatagcctg ctgatgaaca acgacagcct
    12961 gttacaggca gcgcagcatt cactaacaat ggcggtgttt tcggcgacat ttgctacgct
    13021 tatcggttca ctgacggcag ttgcgctgta ccgttatcgc tttcgtggta agccgttcgt
    13081 tagcggaatg ctgtttgtgg tgatgatgtc accagatatc gtgatggcga tttctctgct
    13141 ggtgctgttt atgctgctgg gtattcagct tggcttctgg tcgctgctgt tctcgcatat
    13201 caccttctgc ctgccgtttg tggtggtgac ggtgtattcg cgcctgaaag gttttgacgt
    13261 gcggatgctg gaagcggcga aagatctcgg tgccagtgaa tttaccattc tgcgaaaaat
    13321 cattctgcca ctggcaatgc ctgcggtggc ggcgggctgg gtgttaagct ttaccctgtc
    13381 gatggacgat gtggtagttt cttcgttcgt caccggacca agttatgaaa ttctgccgtt
    13441 aaaaatttat tcgatggtca aagtcggcgt atcgccggaa gttaacgcgc tggcaaccat
    13501 attactggtg ctgtcgctgg tgatggttat tgccagccag cttattgctc gtgataaaac
    13561 gaaaggtaac acaggggacg ttaaatgaaa aaatggtcac gccacctgct cgcggcgggt
    13621 gctctggcac tgggcatgag cgccgctcac gccgatgaca acaacacgct gtatttctac
    13681 aactggaccg agtacgtgcc gccaggactg cttgaacagt tcaccaaaga aaccggtatt
    13741 aaggttatct attcgactta cgagtcgaac gaaaccatgt atgccaagct gaaaacgtac
    13801 aaagacggtg cctatgacct ggtggttcct tcaacctatt acgtcgataa aatgcgtaaa
    13861 gaagggatga tccagaagat cgacaagtcg aagttaacga acttcagcaa tctcgatcca
    13921 gacatgctca ataagccgtt tgacccgaat aacgattact ccattccgta tatctggggt
    13981 gcgacggcga ttggtgttaa cggtgatgcg gtggatccga aatctgtcac cagctgggcc
    14041 gatctgtgga agcctgagta caaaggcagc ctgctgttga ccgacgatgc ccgtgaagtg
    14101 ttccagatgg cgctgcgtaa gctgggctac tccggtaaca ccaccgatcc gaaagagatt
    14161 gaagctgcat ataacgagct gaaaaaactg atgccaaacg tggcagcctt taactccgat
    14221 aacccggcta acccgtacat ggaaggcgaa gttaacctcg gcatgatctg gaacggttct
    14281 gctttcgttg cacgccaggc gggtacgcca attgacgtgg tatggccgaa agaaggcggc
    14341 attttctgga tggacagcct ggcgatcccg gcaaatgcca aaaacaaaga aggcgcgctg
    14401 aaattgatca acttcctgct gcgcccggat gtggcaaaac aggttgccga aactatcggt
    14461 tatccaacgc caaaccttgc ggcgcgtaag ctgttaagcc cggaagtggc gaacgataaa
    14521 acgctctacc cggatgctga aaccattaaa aatggtgaat ggcagaatga cgttggcgca
    14581 gccagcagca tttatgaaga gtattatcag aagctgaaag caggacgtta attcagcaag
    14641 cgggcggtgg cattaccgcc cgttttttat ttagcaagga tgtggctaat gagtcaggac
    14701 agtaaagtgt tatcccgcgc ttttctcggt atcggtctga tattgctcgt tatttctgca
    14761 atcatctttt attaccattt tacttttacc aaaagcgcgg ttcatacaga aggaatcatt
    14821 gttgatgccg tttggtacaa caatcactca aacgacgtcg atgataatgg ttcctggtat
    14881 ccagtggttg cctttcgccc tacgccagat tacacgctaa ttttcaattc gaatatcggt
    14941 agtgattttt atgaagatag cgaaggtgac agagtcaatg tctattatcc gccagggcat
    15001 ccagaacaag ccgaaataaa caatccctgg gttaattttt ttaaatgggg ctttgttggc
    15061 atagcaggca ttattttcgg tagtgtcggt ctgataatca cgctcccctc caccaaaaaa
    15121 tctcgcagga aacggaagtc ccgcccatga aaaaattcat tatactgctc agtttactga
    15181 tactcctgcc tctggtagca accagtaaac cattgattcc aataatgaag acacttttta
    15241 cggatgttac cggtactgta ccggatgctg aagagatcgc acgtaaagcc gagctttttc
    15301 gccaacaaac gggaattgca ccgtttattg tgattttacc cgacattaat aatgaagcca
    15361 gtctcagaca aaatggtaaa gcgatgctgg cgcatgcgtc atcttcattg agtaatgtaa
    15421 aaggaagtgt tctgttacta tttactaccc gcgaaccacg gttaattatg atcaccaacg
    15481 gccaggttga aagtggtctg gacgataaac atctcggcct tctgatagaa aatcacacgc
    15541 tggcttattt aaatgcagat ctctggtatt agggaatcaa taatgcattg gctgttctac
    15601 aagcacagat attaaaacaa ccgacgccgc cactgacgta ttatccgcat ccaggacaac
    15661 agcatgaaaa tgctcccccc ggcagcacca acacattagg ttttatcgcc tgggctgcga
    15721 cgtttatctt attttcctgg atcttttgct acacgactcg ttttatttat gcgttaaaat
    15781 ttgcagtggc gatgactatt gccaatatgg gttatcaggc tttatgtttg tatatcgata
    15841 atagctttgc gattaccaga ataacgccac tctgggcagg gctaattggt gtctgtacgt
    15901 ttattgccgc attgttattg acgagtaaac gataaaagag gccggaaaag tgcgcgtgta
    15961 ttatgccgga tggggcgcga acgccttatc cggcccacaa aacccgcaaa ttcaattaat
    16021 tgcgtcccct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
    16081 cccttcagca acttttcaac aaactcaggc accacctggc ttgccgggcc gtaatatttc
    16141 tcggcaaatt cattaccgac ctgacttggt tcgagattca gttccacggt gtgcgcgcca
    16201 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
    16261 gcaatgaaaa tatcagccat cgacaacgcc atataaattt catccatgcc gagtggcatt
    16321 tcgccaaacc aaacgacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta
    16381 tcttctggcg taacgtctcc ggtccagtcg agaacctgac cactttgcga acaacgaact
    16441 ttcagcaatt ccccatgcat atgaatgaca ttggtattac ctgcgcgttc atgcaggttg
    16501 tctatattct gcgttaccag caaaaagcga tcgccgaggg cgtcttgcag tttagccagc
    16561 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
    16621 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
    16681 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
    16741 gaaatccctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
    16801 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
    16861 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
    16921 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
    16981 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
    17041 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
    17101 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
    17161 tgctcatcgc cttgatcata aagcgcaata atttcgggag cctgcaacgg ttgatgataa
    17221 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
    17281 tgaccacagc cgcagcggcg taacgggaaa tccaacccca tcatggttaa cgcatcaacc
    17341 ggcagacgca tatggccaaa ctcgccggta atgtagcttt taccggtaat cggtttgccg
    17401 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac cagtggatat
    17461 tgagtaaatt catcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
    17521 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagcg
    17581 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
    17641 ccgacagaac ctttacagcc aaaacgttgg tcagcttcag ctaccagctc acacactgca
    17701 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
    17761 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
    17821 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
    17881 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
    17941 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaaat caataaaata
    18001 gatatcgctg gagaggaact gatgaccgat caacttttca atccactcaa taatcggggt
    18061 aagttgcagt gaaaccacca caccgataat caccccacac aggctgccga acagccctgc
    18121 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
    18181 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
    18241 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
    18301 gatatcgcga tacatatagc cgtaagtacc aatccaactt ttaatataaa catagctgtt
    18361 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
    18421 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
    18481 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
    18541 cacacgtttt ggctgcatca gtttatgctc gggattcgag ttggggatca taatcgacac
    18601 ccaatccccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
    18661 ttcgcccgct ttaaaattgc gccacgcatc gccctggaca aacgagggta atgcgctcag
    18721 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgaagat tcacgccact
    18781 ttccaccagc ccggtgaaat tgatatacgg cgcggcagcg gcaatacccg gcactttttg
    18841 cacgtgatcc agtgcttcct gccagttagt ccacggctga tccaccgcct cgatttcacc
    18901 atgcggcacc accgccagaa tacggttgtt cagttcgcgt tcaaagccgt tcatcgcgct
    18961 taagccgacg atcaataccg ccacgccgag ggcaatgcca atggtagaaa taacggagat
    19021 cagcgacacc atgccgccgc gccgccgtcc gcgactaaaa cgcaggccaa ttaataacga
    19081 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
    19141 cgcatttcta actggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
    19201 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtcggcgtta
    19261 cgcgcatcga ggttaccggt aggttcatcc gccagtacca ggcgcgggtt attcaccagc
    19321 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
    19381 gcacgatgat ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
    19441 ggctttttct tgccaatcag cagtggcata gccacgtttt ccagggcagt aaaatccggc
    19501 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
    19561 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
    19621 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
    19681 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
    19741 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
    19801 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
    19861 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
    19921 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
    19981 aagcagtgcg ccaagcgccg caccgaggat cgcaccgata atcccggcgc tggccccttg
    20041 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac
    20101 ttcgccctgc ttctccatta ccatcagccc tagtgaggta ataatgttaa acgccgcaac
    20161 ggcgacaatc aggctcagca gcaagcccat catatttttt tccatgcgta cggcctggaa
    20221 cagctcgcct ttacggtcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
    20281 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
    20341 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
    20401 gacttcactg ttggcggcga aagtgccaat cacattgaac aggcgctggc ttggaatacg
    20461 ccccatcggc gtgaactggc tggcagatgg taccatcacg cggatttgat caccgcgatt
    20521 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
    20581 gtcagtttgt ttcacattga ccagatacgg tgtaagtgga tctttttgcg ccgggtcgat
    20641 accgagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
    20701 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
    20761 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
    20821 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
    20881 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
    20941 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
    21001 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
    21061 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
    21121 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
    21181 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
    21241 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
    21301 ctggtggtga tttatcactc ggtcattacc ttttatccgc atctgaccac ttttcagcat
    21361 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcccccttt
    21421 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
    21481 ccgtggggaa attgtctcga tatacggatc tggaacatct tctgggtgct ggcgctttgg
    21541 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
    21601 ttaagcaatg cctccaatgc cgcttatgcc gattccaccg gtgagttcct gcacgggatg
    21661 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
    21721 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
    21781 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgctttat
    21841 tacagccttg gcgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
    21901 cgccgccatc tgctgatggc ttctttgctt accgttctgg cggtcggtgc ctggttgttt
    21961 actatctcgc tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcaa
    22021 tacgagcaac gattcggtat gcgctccacc agcctgctga atgtgattgg ctccaacacc
    22081 attgctatct acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg
    22141 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttatccc
    22201 tttgtcagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgt
    22261 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ctgccgtcag ttactctcgt
    22321 taaaccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
    22381 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
    22441 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    22501 tctgctgggc gagttaaccg gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg
    22561 tcacgccggt ccggtggtac tcattgcacc agatatgcaa aatgctctgc gtttgcatga
    22621 tgaaatcagc cagttcaccg atcagatggt gatgaatctg gcggactggg aaactcttcc
    22681 ctacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    22741 gctaccgacg atgcagcgtg gcgtactgat tgttccggtg aatacgctta tgcagcgcgt
    22801 ttgcccacac agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc
    22861 acgagatgca ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat
    22921 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    22981 gctgccttat cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt
    23041 cgacagccag cgcacgctgg aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt
    23101 tccgaccgat aaagcggcaa ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    23161 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    23221 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    23281 tgccaatacc ttgctggtga atactggcga tctggaaacc agtgccgaac gtttccaggc
    23341 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc
    23401 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgggt
    23461 gcagctaaaa actgaacatt taccgacaaa agccgcgaat accaatttag gtttccagaa
    23521 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    23581 cctcgagact ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc
    23641 gctgggtgaa ctgctcgcac gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    23701 agccagcgac cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgataa
    23761 ggtgcgtaat ctggcgctga tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg
    23821 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc ttgcggagct
    23881 gcatattggt cagccggtgg tccatctgga gcacggtgtc gggcgctatg ccggaatgac
    23941 cacgctcgaa gcgggcggca ttactggcga gtatttgatg ctcacctatg ccaacgacgc
    24001 caaactgtat gttccggtgt cgtcactgca tctgattagc cgttacgcag gtggcgcgga
    24061 agaaaacgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    24121 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    24181 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    24241 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    24301 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    24361 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgttggtgcc
    24421 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    24481 ggtacgtatc gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    24541 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    24601 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    24661 tcataaagag cgtattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    24721 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc
    24781 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    24841 ggtccgggag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    24901 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    24961 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    25021 ccatcatcaa cgtttcaacg tgctggtttg taccaccatt atcgaaaccg ggatcgacat
    25081 cccgacagcc aacaccatta tcattgaacg tgcggatcac ttcggtctgg cgcagctgca
    25141 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggt tgctgacgcc
    25201 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    25261 agatctcggt gcaggctttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    25321 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    25381 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    25441 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    25501 tcctgacgtg aatacgcgtt tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    25561 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    25621 gcgtatcctg ctggatgttg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa
    25681 gctggaaggt aatgagaaag gcggggtgat cgaatttgcc gagaagaatc acgttaatcc
    25741 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    25801 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    25861 gtttatgcgt gaactggaag agaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    25921 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    25981 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    26041 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    26101 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    26161 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    26221 aaaaaatata acgtcggctt tctcgctctg ttacaggcta accccggcgt tgatccttac
    26281 gtaccgcgcg cggacagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    26341 cgcgaaggca ttgtgatcaa cattgcggag ctgcgtctct attactaccc gccgggtaaa
    26401 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    26461 acaatggtga ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac
    26521 atccgcgccc gttataaagc acagggaatt gagttgcctg cggtagtgcc ggctggaccg
    26581 gataacccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    26641 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    26701 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    26761 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    26821 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    26881 gcaatgcaat catttaaaga tgcagcacaa actgacgcag aagtgatgca acatgtgatg
    26941 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg
    27001 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    27061 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    27121 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    27181 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    27241 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    27301 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    27361 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgatag atgtgatctg
    27421 gatcacatac aagatatttt ttataacaat catta
//
LOCUS       DAPHDX010000057        27021 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPHDX010000057 DAPHDX010000000
VERSION     DAPHDX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.057"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            205..975
                     /locus_tag="RH058_002335"
     CDS             205..975
                     /locus_tag="RH058_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV3137915.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            966..1439
                     /locus_tag="RH058_002336"
     CDS             966..1439
                     /locus_tag="RH058_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV3137916.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1546..2085
                     /gene="dnaT"
                     /locus_tag="RH058_002337"
     CDS             1546..2085
                     /gene="dnaT"
                     /locus_tag="RH058_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV3137917.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVATEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2088..2825
                     /gene="dnaC"
                     /locus_tag="RH058_002338"
     CDS             2088..2825
                     /gene="dnaC"
                     /locus_tag="RH058_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV3137918.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2874..3368
                     /locus_tag="RH058_002339"
     CDS             2874..3368
                     /locus_tag="RH058_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV3137919.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3622..5913
                     /gene="opgB"
                     /locus_tag="RH058_002340"
     CDS             3622..5913
                     /gene="opgB"
                     /locus_tag="RH058_002340"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV3137920.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRDDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6052..7074)
                     /gene="lgoD"
                     /locus_tag="RH058_002341"
     CDS             complement(6052..7074)
                     /gene="lgoD"
                     /locus_tag="RH058_002341"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV3137921.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7213..8127
                     /locus_tag="RH058_002342"
     CDS             7213..8127
                     /locus_tag="RH058_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3137922.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVLVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8342..9703
                     /locus_tag="RH058_002343"
     CDS             8342..9703
                     /locus_tag="RH058_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3137923.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9752..11416)
                     /gene="tsr"
                     /locus_tag="RH058_002344"
     CDS             complement(9752..11416)
                     /gene="tsr"
                     /locus_tag="RH058_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV3137924.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11535..11981)
                     /gene="hpaR"
                     /locus_tag="RH058_002345"
     CDS             complement(11535..11981)
                     /gene="hpaR"
                     /locus_tag="RH058_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDV3137925.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12253..13542
                     /locus_tag="RH058_002346"
     CDS             12253..13542
                     /locus_tag="RH058_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDV3137926.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13539..15005
                     /gene="hpaE"
                     /locus_tag="RH058_002347"
     CDS             13539..15005
                     /gene="hpaE"
                     /locus_tag="RH058_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3137927.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15007..15858
                     /gene="hpaD"
                     /locus_tag="RH058_002348"
     CDS             15007..15858
                     /gene="hpaD"
                     /locus_tag="RH058_002348"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDV3137928.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15868..16248
                     /locus_tag="RH058_002349"
     CDS             15868..16248
                     /locus_tag="RH058_002349"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDV3137929.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16359..17162
                     /gene="hpaH"
                     /locus_tag="RH058_002350"
     CDS             16359..17162
                     /gene="hpaH"
                     /locus_tag="RH058_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDV3137930.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17173..17961
                     /gene="hpaI"
                     /locus_tag="RH058_002351"
     CDS             17173..17961
                     /gene="hpaI"
                     /locus_tag="RH058_002351"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDV3137931.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            18136..19512
                     /gene="hpaX"
                     /locus_tag="RH058_002352"
     CDS             18136..19512
                     /gene="hpaX"
                     /locus_tag="RH058_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDV3137932.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            19522..20412
                     /gene="hpaA"
                     /locus_tag="RH058_002353"
     CDS             19522..20412
                     /gene="hpaA"
                     /locus_tag="RH058_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDV3137933.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            20663..22225
                     /gene="hpaB"
                     /locus_tag="RH058_002354"
     CDS             20663..22225
                     /gene="hpaB"
                     /locus_tag="RH058_002354"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDV3137934.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            22243..22755
                     /locus_tag="RH058_002355"
     CDS             22243..22755
                     /locus_tag="RH058_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDV3137935.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23148..25298
                     /gene="btsT"
                     /locus_tag="RH058_002356"
     CDS             23148..25298
                     /gene="btsT"
                     /locus_tag="RH058_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV3137936.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25429..25632
                     /locus_tag="RH058_002357"
     CDS             25429..25632
                     /locus_tag="RH058_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV3137937.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25643..26599
                     /gene="yjiA"
                     /locus_tag="RH058_002358"
     CDS             25643..26599
                     /gene="yjiA"
                     /locus_tag="RH058_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV3137938.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(26686..26877)
                     /locus_tag="RH058_002359"
     CDS             complement(26686..26877)
                     /locus_tag="RH058_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDV3137939.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
BASE COUNT         6371 a         7281 c         7041 g         6328 t
ORIGIN      
        1 aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag ataattcgct
       61 ttttatcacc gagcttgtcc cgataaccag gattaatcag cgcaattttg atcttaacct
      121 accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa caatacgcag
      181 cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt aacacggctt
      241 tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag
      301 ctttcctcac gactgggtcg ggcgttgggg atggacagcg tcgaaagttc tatctcttcg
      361 aacgccatag tgctgacaac cattaaagat gggcaatgcc tgacatcgac acgtaaaaat
      421 cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt gattctggcg
      481 gagcaccatc tgctggatta caaaggcgta gagaaacgat ttagccaaat tcagccatta
      541 cgttacccaa gatggctggt agccttaatg gttggccttt cttgtgcctg tttctgtaaa
      601 ctcaataaag gtggctggga tggtgccgtc atcaccttct ttgccagtac ggccgcgatg
      661 tatatccgcc agctgctggc acaacgtcat cttcatccac aaatcaactt ttgccttacc
      721 gctttcgccg ccaccaccat ttccggattg cttttgcaac ttcccacttt cagcaataca
      781 cccactatcg cgatggccgc cagcgttctg ctgctggtgc cgggatttcc gttgattaat
      841 gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg ggcgatcgcc
      901 agtctgctga cactggctac ctgcgtcggc gtagtgatgg cactgacgat ttgggggcta
      961 cgcggatggg tgtgatcgaa tttctgttag cgttggcgca ggatatgatc ctcgccgcca
     1021 ttcctgcggt cggctttgcg atggtgttca acgttcccgt tcgggcgtta cgctggtgtg
     1081 cgctgcttgg cgcgattggt catggttcac gaatgatctt aatgaccagc gggctgaata
     1141 ttgagtggtc aacttttatg gcttctatgc tggtcggcac cattggtatt caatggtcgc
     1201 gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct atgttcccgg
     1261 gcatatcggc ttataccgca atgatttcgg cggtaaaaat cagccagtta ggttacagcg
     1321 aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt
     1381 tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa
     1441 attgcctcgc ttaagcacac ggatgagaga cagcctcctc tcctccgtgt gttactataa
     1501 aagttatctc ccttctcgtt catcgttcca tatttgagaa acagtatgtc ttccagagtt
     1561 ttgaccccgg acgtcgttgg tattgacgcc ctggtacacg atcaccaaac cgttctggca
     1621 aaagctgaag gcggtgtggt tgccgtattt gctaacaatg ccccggcgtt ttatgccgtc
     1681 acgcctgcac gcctggctga actgctggcg ctggaagaaa agctggcgcg tccgggaagc
     1741 gatgtcgctc tggacgatca actctatcag gaaccgcaag ccgctcccgt tgctgtcccc
     1801 atggggaaat tcgccatgta tccggactgg caacccgatg ccgactttat ccgcctggcg
     1861 gcgctatggg gcgtggcgct aagagagccg gtggccaccg aagaactggc ctcattcatt
     1921 gcctactggc aggcggaagg taaagtcttt caccatgtgc agtggcaaca aaaactggcg
     1981 cgcagcctgc aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg
     2041 gtcagcgaac ctgacagcca aattccacca ggattcagag ggtaacgatg aaaaacgttg
     2101 gcgacctgat gcaacgcctg caaaaaatga tgcctgccca tatcaaacct gcattcaaaa
     2161 cgggtgaaga gctactggcc tggcagaaag aacaaggagc gatccgctcc gccgctctcg
     2221 aacgtgaaaa tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt attcggccac
     2281 tgcatcagaa ctgctccttt gagaactatc gcgttgagtg tgaagggcag atgaatgcgt
     2341 taagcaaagc gcgccagtac gtcgaagagt ttgacggcaa catcgccagc ttcatctttt
     2401 ctggtaagcc aggaaccggc aaaaaccatc tggcggcggc aatctgcaac gagctgctgc
     2461 tacgcggtaa atccgtattg atcatcaccg tggccgatat tatgtcggcg atgaaagata
     2521 ccttcaggaa tagcggtacc agtgaagaac aactgcttaa cgatctgagc aacgttgatc
     2581 tgctggtgat cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatca
     2641 accagatcgt cgatcgccgc tcttcttcca aacgcccaac cgggatgctg accaacagta
     2701 atatggaaga gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta
     2761 acagtttgtg ggtgatcttc aactgggata gctaccgtag ccgggtaaca ggtaaagagt
     2821 attaatcccc gtttctggcg gtatactgcg acacaattcc accttgagta atgatgatga
     2881 aaactgttaa acatcttctg tgctgtgcca ttgccgccag cgcattaatt tccaccgggg
     2941 tgcatgctgc gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa
     3001 acagcacgac acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg
     3061 ggaaccaggc cttaagcgca gataacatga acaacgccgc aggcattctg caatactgcg
     3121 cgaagcaaaa gctggcgtcg gtaaccgatg ccgaaaacat caagaaccag gtgctggaaa
     3181 agctgggcct gaacagtgaa gagcaaaaag aagacaccaa ctatctggat ggcattcagg
     3241 gtttgctgaa aaccaaagat ggtcagcaac tcaatctgga taacatcgga acgactccgc
     3301 tggcagaaaa ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg ctgaacttca
     3361 tttcctgaac cgcgaaacgc cgcgttttag ctgttacccg gctgacgcgg ctctctcctg
     3421 cccttttccc tgctcctgga cggttttccc cctgttacac gggaagcgac cgcgatcaaa
     3481 aaagtctgtt tataaactaa ttcctaacaa catcgcattt ttttgacact agaattgcag
     3541 caatatggcg acgccattac attgtgcttc tcaaaaagct taaacgttgg ctaaacggcc
     3601 cggtttagtg aggatcgccc gttgtcagaa ctactctctt tcgccctttt tctcgcctct
     3661 gtgctgattt acgcatggaa agcgggacgt aacacctggt ggtttgcagc cacattaacg
     3721 gtgctggggc tatttgtcgt tttaaatatc accctgtttg ccagcgacta ttttactggc
     3781 gatggtatta acgacgcggt tctctatacc ttaaccaaca gcctgaccgg tgctggcgtc
     3841 agcaaataca tcctgccggg tatcggcatt gtgctggggc tgacagcggt gttcggtgcg
     3901 ctgggctgga tcctgcgccg tcgtcgccat catccgcacc attttggtta cagcctgctg
     3961 gcgctcttac tggcgctggg ttcggtggac gccagcccag catttcgtca gataacggaa
     4021 ctggtgaaat cccagtcacg cgatggcgac ccggactttg cggcttatta taaagagccg
     4081 tcgaaaacta tccctgaccc gaaactcaac ctggtttata tctacggcga aagtctcgag
     4141 cggacctatt ttgataacga agctttcccg gatctcacgc ctgaactggg cgcgttgaaa
     4201 aatgaaggcc tggatttcag ccacacgcag cagctgccag gaacggatta cacgattgcg
     4261 ggcatggtgg cttctcagtg cggcataccg ctgtttgccc cctttgaagg caacgcctcc
     4321 gcctctgtct ccagcttctt cccgcagaac atctgtctgg gcgatatcct gaaaaactcg
     4381 ggttatcaga actatttcgt gcagggcgcg aatctgcgtt ttgccggtaa agatgtgttc
     4441 ctgaagtcgc atggcttcga ccacttatac ggctcagaag agctgaaaag cgtggtggcc
     4501 gacccgcact atcgcaacga ctggggattc tacgacgata ccgttctcga tgaagcgtgg
     4561 aaaaagtttg aagagctttc ccgctcaggt cagcgattct cactgtttac cctgacagtc
     4621 gatacccatc acccggatgg ttttatctcg cgtacctgta accgcaaaaa atatgatttt
     4681 gacggtaagc cgaaccagtc attcagcgcg gtaagttgca gccaggagaa tatcgcggcg
     4741 tttatcaaca aaatcaaagc gtcaccgtgg tttaaagata ccgtcatcgt cgtctcttct
     4801 gaccatttag cgatgaacaa cacggcgtgg aaatacctta ataagcagga tcgcaataac
     4861 ctgttttttg tcattcgtga cgacaagccg cagcaagaga cgctggcagt gaagcgtaac
     4921 acgatggata acggcgcgac ggtgctggac attctcggtg gcgataacta tctcggactt
     4981 ggtcgtagca gtttatccgg gcagtcgatg tcggaaatct tcctcaatat caaagagaaa
     5041 acattggcgt ggaagccgga tatcatccgc ctgtggaaat tccctaaaga gatgaaagag
     5101 ttcactatcg accagcagaa aaacatgatt gccttctcgg gtagccattt ccgtttgccg
     5161 ctgctgttgc gggtttcaga caagcgcgta gaaccgctgc cggaaagtga atactcagca
     5221 ccgctgcgtt tccagctggc cgatttcgct ccacgcgaca atttcgtctg ggttgaccgt
     5281 tgctacaaga tggcacaact ttgggctccg gaactggcac tctccaccga ctggtgtgtc
     5341 tcgcaggggc agcttggcgg tcagcaaatt gtccagcatg ttgataaaac aatgtggaag
     5401 ggcaaaacgg catttaaaga tacggtcatc gacatggcgc gttacaaaag caatgtcgat
     5461 acgctgaaaa ttgttgataa cgatattcgc tacaaagccg acagtttcat cttcaacgtc
     5521 gccggtgcgc cagaagaggt gaaacagttt agcgggattt cccgtccgga gtcgtggggc
     5581 cgctggtcca acgctcagct gggcgatgaa gtaaaaatcg agtacaagca tccgctgccg
     5641 aagaaatttg acctggtgat taccgccaaa gcatacggca ataacgccag ccgtcctatt
     5701 ccggtacgcg taggcaatga agaacaaacc cttgtgctgg gcaatgaagt gaccaccacc
     5761 acactgcatt tcgataaccc aaccgatgcc gacacactgg taattgtgcc gccggaacct
     5821 gtctcaacca acgaagggaa tatcctcgga cactcgccgc gtaagctcgg gatcggcatg
     5881 gtggaaatta aagtggtgga acgtgaagga taattcctga ctgatgtgta ggtcgcataa
     5941 ggtgcgacct acacaccttc tgcggcctac aaaacatcat tgagaaacag aaaaaaacag
     6001 cgcccaacac ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt
     6061 aattacaccc ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag
     6121 cgtggcgaac ggatagcgat gggttaacat catgtcggca gtgatttttc cttccgccat
     6181 cagtcgaccg actttagcaa aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt
     6241 ttctttttta tggaattccg gatcggagaa ctgcaactca cctttaaaca ggccgacaaa
     6301 taccaccgtg ccgccgtgac gaatcaaatt cacggtgtta ttcatcgcat gttgattacc
     6361 tgtcgcgtcg atcactttct gcgccagcga accaccaaac tgcgcccgta gctgcgcgtc
     6421 aaaatcttcg gctgacgggt ccagtaaagg taattccaga cgcgttgcca catgttcacg
     6481 gcgcgccgga ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc
     6541 cgcgcccaga ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc
     6601 tgcgcgacgc accgcatgag cgctaatagc gaaaggttca atcaatgctg ccgcctgcgg
     6661 gtcaataccg tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc
     6721 gccatcctga tgcacgccaa tgactgaaat tttttcacag caattggtac gcccgctttt
     6781 acacgccggg cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt
     6841 aagatcagca atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac
     6901 acgtggataa ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat
     6961 cccgacagac ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg
     7021 tttccagact aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg
     7081 gctctccatt ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa
     7141 aatctgtttt gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac
     7201 aaggaggaaa atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt
     7261 gatgatatgg cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa
     7321 atgtacaaca tcagccgtac tacagtgcgt catattctca gccacttacg cgaatgcggc
     7381 gtcttaacgc aggttggaaa cgactatgtt attgcgcgta aacctgacca tgatgacggt
     7441 ttcgcctgta ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc
     7501 acgatgataa accagcgcca gttacgccca ggggaaacgt tttctgaact gcaactggct
     7561 cgggccgcgg gtgtcagccc ggttctagta agagaatatc ttttaaaatt cggccgttat
     7621 aatcttattc acagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac
     7681 gccgagcagc tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc
     7741 aacctgcctg atcatgatcc ccgctggctg caggcgaaaa ccatgctcga acgtcatcgg
     7801 ctactgcgtg acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt
     7861 cactccttac tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc
     7921 tcagtcatct ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc
     7981 attgccgtcg atgagcatat gaccattctc agcgctctga tttgccgcag tgaccttgat
     8041 gccacccttg cgctccgtaa ccatttgaat tcagctaaac aatcaatgat tcgatccatt
     8101 aatgagaaca cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt
     8161 gcacggtttt atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag
     8221 tcgactaaaa atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat
     8281 gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg
     8341 cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc
     8401 agatattccc gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca
     8461 aaccaccgcc atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc
     8521 gctgtcggta gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg
     8581 cgctttgctc tccgtgtttt cactcgctta cgggattgcg caacttcctt gcggcccact
     8641 attggatcgt aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt
     8701 ccaggcaatg tctggcatgg tgcacaactt tacgcagttc gtgttggtgc gtatcggtat
     8761 ggggattggt gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg actggttcaa
     8821 catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc
     8881 cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat
     8941 taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg
     9001 cgagcacgta gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc
     9061 ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gtacaatgtg
     9121 gggaatgatg ctcggattca gtggtatcaa ctacactgcg tggctgtatc tggcctggct
     9181 tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatggcggc
     9241 tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct
     9301 ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt
     9361 ctgttctgcc gcctttacgc tggtcgtacc acaagcgaca acatccatga cggcggttct
     9421 gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat
     9481 ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag
     9541 cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata ccacccactc
     9601 attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac tggcgtacat
     9661 cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt atgcccgata
     9721 acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc tcctcgctat
     9781 ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca gccgatgttt
     9841 cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac
     9901 tggcctgctc ttccagcgcg gcggcggcag cggcagattc ttccaccagc gcggcgttct
     9961 gttgagttac ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct
    10021 gctcatcaga agcagaagca atttcgccca taatgtccgt cacgcgggtc acggcgctga
    10081 caatctccgc cattgtttcc ccggcgcttt cgaccagcgt agagccaaca tccactttcc
    10141 ccaccgagtc ttcaataagg cttttaattt cacgagccgc ctgagcgcta cgctgggcca
    10201 gattacgcac ttctcccgca accaccgcaa aaccgcgccc ttgctcaccc gcacgcgctg
    10261 cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa ggcaatgccg tcaattacgc
    10321 tgataatatc ggcgattttc tgcgaactgg tggagatatc gcgcatagtc tgcaccacgt
    10381 tatctaccac tttgccgccc cgttgcgccg tttcagaagc acttaatgcc agatggctgg
    10441 cctggcgcgc attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg
    10501 ccgtctcttc cagcgaagcg gcctgttgct cggtgcgcga agagagatcg ttattgccgg
    10561 tagcgatttc gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg
    10621 tacgcatcag ctctccctgc atatggcgca aactctctgc cagttgcccc atctcattag
    10681 agccatccac ctcaatcggt ttcaccagat cgccgcctgc aatatgacgg atgctgtcaa
    10741 tcaggcgatt cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga
    10801 ccgccagtac gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat
    10861 tgttatcgct gacggcgata tcatagagcc gatcgttttg ctccatgtaa gccacatact
    10921 gcttctcgaa accgtcttga tatccctggg tcggctgatc aaagaactcg ttgattttgc
    10981 ctgcacctaa cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt
    11041 tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatccg
    11101 cccagttttt ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg
    11161 ttgaaccgct accaatatta ttctgatcca tcatgtagcg gatacccgcg cggttgaggg
    11221 tgttacgcgt ctgcaacaac gcgacccagc tgccattcag cgtggattgc tgctggcgaa
    11281 tggtttgtaa aacagtgaaa ttttctttgt cattctttaa ggcattaaag aacagaccgc
    11341 ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct
    11401 tgatacgttt taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctggaa
    11461 agcaaaaact ttatgaaaag gtgtaacccg ccatccatgg aaaaaccagg cgctggttat
    11521 cagatactaa aaagttattc attatcgccc gggatggctt cttgtcggga attgcccaga
    11581 gcaataaatt cctctaacag atgggttaac tgttgcattt tctcggcagt aaactgagct
    11641 tcaatctgcc gataggcttc ttcaatctgt gtctgagcgc ggttatacag cgcctgcccc
    11701 tctttggtta gcgagatata gagcttccgc tgatcattga tcggctttaa tcgcaacact
    11761 aaaccgtcgc gttccatccg cgtaaggatc ccggtcaggc ttgggcgcaa aatgcatgcg
    11821 cgatacgcca gatcgtgaaa atccattgat gggctttccg ccagaatacg cacgatgcgc
    11881 cactgttgct cagtcaaatt atgccgttta acgattgggc gaaaataact catcgccgct
    11941 tctcgcgcct ggagcaacgc aatggttaga gagtcgtgca ttatctttcc cctgaaaaat
    12001 tcaaaatcat taatatagaa acagttaatc atcacgttac cgggactgcg ctgcccctgc
    12061 aattcttcat tttatcaaaa ttgagcatgt tttgttaact tgctgatatt taatatttta
    12121 ttatctttaa atgtaaatag tttgttaatt agatcacatt tacatcactt acttttgcca
    12181 aactacgcaa tcgggaataa aagtaatcat taacatatta atgattaaaa tgatcgaatc
    12241 cgaggagtgg taatgaaagg cactatcttc gccgtagcgt tgaaccatcg cagccaactt
    12301 gatgcatggc aggaagcgtt ccagcaatcc ccctacaaag ccccgcctaa aactgcggtc
    12361 tggtttatta aaccgcgcaa tacggtgatt ggttgcggtg aaccgattcc ctttccacag
    12421 ggtgaaaagg tactgagcgg tgcgactgtt gcgctgattg tgggaaaaac ggcgacgaaa
    12481 gtacgtgaag aagatgcggc agagtacatc gccggatatg cgctggctaa cgacgtcagc
    12541 ctgccggaag agagctttta ccgcccggca atcaaagcaa aatgtcgtga tggattctgc
    12601 cccattggcg aaaccgtggc tctcagcaat gtcgataatc tgaccatcta taccgagatc
    12661 aacgggcgtc ctgccgatca ctggaatacc gccgatttac aacgtaacgc cgcgcagttg
    12721 ctgagcgccc tgagcgaatt tgccacgctg aatccaggcg acgccattct gctcggcacg
    12781 ccacaggcgc gcgtggaaat acagccaggc gatcgcgttc gtgttctcgc agaaggtttc
    12841 ccgccgctgg aaaatccggt agtggacgaa cgtgaagtga ccacgcgcaa gagcttccca
    12901 acgcagccac acccgcacgg cacgctgttt gccctcggcc tgaactacgc cgaccacgcc
    12961 agcgaactgg aatttaagcc accggaagaa ccgctggtgt tcctgaaagc gccaaatacc
    13021 ctcactggcg ataaccagac ctccgtgcgt ccaaacaata ttgaatacat gcactatgaa
    13081 gcggagctgg tggtagttat tggcaaacag gcgcgtaacg tcagcgaagc cgatgccatg
    13141 gattatgtcg cgggctacac cgtgtgtaac gactacgcca ttcgcgacta tctggaaaac
    13201 tactaccgcc ctaacctgcg ggtcaaaagc cgcgacggac tgacgccgat gctttcaacc
    13261 atcgtgccga aagaggcgat cccggacccg cataatctga cccttcgcac cttcgtcaac
    13321 ggcgagttac gccagcaagg caccaccgcc gatctgatct tcagcgtgcc cttcctgatc
    13381 gcctacttaa gcgaatttat gaccctgaat ccgggcgaca tgatcgccac cggcacacca
    13441 aaaggcttat ctgacgtggt gcctggcgat gaagtagtgg tggaagtaga aggcgtgggc
    13501 cgcctggtga accgaattgt gagtgaggaa acagcgaaat gaaaaaagta aatcattgga
    13561 tcaacggcaa aaatgttgca ggtaacgact acttccagac caccaatccg gcaacgggtg
    13621 aagtgctggc ggatgtggcc tctggcggtg aagcggagat caatcaggcg gtagcggcag
    13681 cgaaagaggc gttcccgaaa tgggccaatc tgccaatgaa agagcgtgcg cgcctgatgc
    13741 gccgtctggg cgatctgatc gaccagaacg tgccagagat cgccgcgatg gaaaccgcgg
    13801 acacgggcct gccaatccat cagaccaaaa atgtgttgat cccacgcgct tcccacaact
    13861 ttgaattttt cgcggaagtc tgccagcaga tgaacggcaa gacctatccg gttgacgaca
    13921 agatgctcaa ctacacgctg gtgcagccgg tgggcgtttg tgcgctggta tcgccgtgga
    13981 acgtaccgtt tatgaccgcc acatggaagg tcgcgccgtg tctggcgctg ggcaataccg
    14041 cggtactgaa aatgtcggaa ctctccccgc tgaccgctga ccgcctgggt gagctggcgc
    14101 tggaagccgg tattccggca ggcgtgctga acgtggtaca gggctacggc gcaaccgcag
    14161 gggatgcgct ggttcgtcat catgacgtac gtgccgtgtc gttcaccggc ggtacggcca
    14221 ccgggcgcaa catcatgaaa aacgccgggc tgaaaaaata ctccatggaa ctgggcggta
    14281 aatcgccggt gctgattttt gaagatgccg atattgaacg cgcgctggac gccgccctgt
    14341 tcaccatctt ctcgatcaac ggcgaacgct gcaccgccgg ttcgcgcatc tttattcagc
    14401 aaagcatcta cccggaattc gttaaacgct ttgccgaacg cgccaaccgt ctgcgcgtgg
    14461 gcgatccgaa cgatccgaat acccaggttg gcgcgcttat cagccagcag cactgggaaa
    14521 aagtctccgg ctatatccgt ctcggcattg aagaaggcgc aaccctgctg gcgggcggcc
    14581 cggataaacc gtccgacctg cctgcgcacc tgaaaggcgg caacttcctg cgcccaaccg
    14641 tgctggcaga cgttgataac cgtatgcgcg tcgcccagga agagattttc gggccggtcg
    14701 cctgcctgct gccgtttaaa gacgaagctg aaggcttacg cctggcaaac gacgtggagt
    14761 acggcctcgc gtcgtacatc tggactcagg atgtcagcaa agtgctgcgt ctggcgcgtg
    14821 gcattgaagc tggcatggtg ttcgtcaaca cccagaacgt gcgtgatttg cgccagccgt
    14881 ttggcggcgt aaaagcctcc ggcaccgggc gtgaaggcgg tgagtacagc ttcgaagtgt
    14941 tcgcggaaat gaagaacgtc tgcatttcca tgggcgacca tccaattccg aaatggggag
    15001 tctgatatgg gtaagttagc gttagccgcc aagatcacgc acgtaccgtc gatgtatctc
    15061 tctgaactgc cagggaaaaa ccacggttgc cgccagggcg cgatcgacgg gcataaagag
    15121 atcagcaagc gttgccggga aatgggcgtc gataccatta tcgttttcga tacccactgg
    15181 ctggtcaaca gtgcttatca catcaactgt gcagaccatt ttgaaggcgt ctacaccagc
    15241 aacgagctgc cgcattttat ccgcgatatg acctacaact acgagggcaa cccggagttg
    15301 gggcagctta ttgccgatga agccttaaag ctcggcgtgc gggcaaaagc gcacaacatt
    15361 cccagcctga aactggaata cggcacgctg gtgccaatgc gctacatgaa tgaagacaag
    15421 cacttcaaag tggtttccat ttcagctttc tgcactgttc acgattttgc cgacagccgc
    15481 aagctgggcg aggcgattct gaaagcgatc gaacagtacg acggcaccgt ggcggtcctt
    15541 gccagcggtt cgttatcgca ccgctttatt gacgatcagc gagcggaaga agggatgaac
    15601 agctacaccc gcgagttcga ccgccagatg gacgagcgtg tggtgaagct gtggcgcgaa
    15661 ggccagttca aagagttctg caatatgctg ccggagtacg ccgactactg ctacggcgaa
    15721 ggcaatatgc acgacacggt gatgctgctg gggatgctcg gctgggataa atacgacggc
    15781 aaggtggagt ttatcaccga gctattccca agctctggca ccggtcaggt taacgctgtt
    15841 ttcccgcttc ccgcgtaagg acgttttatg ccgcacttta tcgttgaatg cagtgacaac
    15901 atccgcgaag aagccgacct gccggggttg ttcgccaaag tgaatccgac gctggcagcc
    15961 acgggtattt ttccgctggc gggtattcgc agccgcgtgc attgggtcga tacctggcag
    16021 atggccgacg ggcagcgtga ttatgccttc gtgcatatga cgttgaaaat cggcgcaggt
    16081 cgcagcctgg aaagccgtca gcaggcgggt gaaatgctgt ttgaactgat taaaacgcac
    16141 ttcgccgccc tgatggagag ccgcctgctg gcgttgtcgt ttgagattga agagctgcat
    16201 ccgacgctga attttaaaca aaacaacgtg cacgcattgt ttaagtagcg cgcagattgc
    16261 ccggtggcgc tgcgcttacc gggcctacaa aaccccaaac cgtacaccgt aggccggata
    16321 aggcgcagcc gcatccggca atgccacagg atatcgctat gttcgataaa catacccaca
    16381 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16441 cactggatta cccggagatc accatcgaag acgcttacgc ggtgcagcgc gaatgggttc
    16501 gcctgaaaat cgccgaaggt cgcacgctga aaggccacaa aatcggcctg acctcgaaag
    16561 cgatgcaggc aagctcgcag atcagcgaac cggattacgg cgcgctgctg gatgacatgt
    16621 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16681 agctggcttt cgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgtct
    16741 acaacgccac ggactacgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    16801 tcgatccgga aacccagcgc ccgcgcaaag tgttcgacac catttctgat aacgccgcca
    16861 acgccggggt gatcctcggt ggtcgtccca ttaagcccga tgagttggat ctgcgttgga
    16921 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    16981 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17041 aactggaagc cgggcaaatc attctcggcg gttcgttcac ccgcccggtt ccggcgcgta
    17101 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17161 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg cccgcagatc
    17221 ggattatggc tggggctgag tagcagctac agcgcagagt tactggccgg agcaggattc
    17281 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccag
    17341 ctacaggcga ttgcgcccta ccccagccag ccggtggtac gtccgtcgtg gaacgatccg
    17401 gtgcaaatca aacaactgct ggacgtcggc acacaaacct tgctggtgcc gatggtacaa
    17461 aacgctgacg aagcccgtga agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17521 ggtgtgggca gtgcgctggc ccgagcctcg cgctggaatc gtattcctga ttacctgcaa
    17581 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17641 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    17701 agcgccgata tgggttatgc cggtaatcca cagcacccgg aagtacaggc cgcgattgag
    17761 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    17821 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    17881 accctgctcg cccgcgccgc tgaagcgctg gcagcacggt ttggcgcaca ggccacgacc
    17941 gttaagcccg gcgtgtatta atgccggatg cgctgcactt atccggccta cactcgcacc
    18001 aaaccgtagg ccggataagg cgtttacgcc gcatccggca aaaagccgta ccaaatcgcg
    18061 gaccggataa ggcgtttgtg cgcatccgcc ataaaccttg tcgtacccta caaaaatccc
    18121 attagaggaa gaaaaatgag cgacacctca cctgccatac cggagagtat cgatccggcg
    18181 aatcagcata aagcgctgac tgccggacaa caggcggtta ttaagaagct gtttcgccgc
    18241 ctgatcgtct ttctgttcgt gctgtttatc ttctcgttcc ttgatcgcat caacatcggc
    18301 tttgccggac tcacgatggg acgcgacctc ggtctgagcg ccaccatgtt tggcctcgct
    18361 accaccctgt tctatgccgc ttatgtcatc ttcggcattc ccagcaacat tatgctgagt
    18421 attgtcggtg cgcggcgctg gatcgccacc atcatggtgc tttggggcat cgcctctact
    18481 gccaccatgt ttgccactgg ccccaccagc ttgtacgtac tgcgtatact ggttggcatt
    18541 accgaagccg gctttctgcc cggcattctg ctgtatttaa ccttctggtt tccggcctat
    18601 ttccgcgccc gtgccaacgc cttgtttatg gtagcaatgc cggtaacgac agcgttgggc
    18661 tcgatcgttt ccggctacat tttgtcgctg gatggcgtca tggcattaaa aggctggcag
    18721 tggctgtttt tgctggaagg cttcccgtcg gtattactcg gcgtcatggt gtggttctgg
    18781 cttgatgact caccggacaa agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa
    18841 gagatgatgg ataacgatcg tctgacgctg gttcagccag agggagccat cagccaccac
    18901 gccatgcaac aacgcagtat gtggcgggag atcttcactc cggtggtgat gatgtatacc
    18961 ctggcgtatt tctgcctgac caacacactt agtgcgatca gcatctggac accgcagatc
    19021 ctgcaaagtt ttaatcaggg cagcagtaat atcaccatcg gcctgctggc cgccgtaccg
    19081 cagatttgta ccattctcgg gatggtctac tggagccgtc actcagatcg ccgccaggaa
    19141 cgaaggcatc acaccgccct tccttatttg ttcgctgccg caggttggtt actggcttcg
    19201 gcaactgatc acaacatgat ccagatgctg gggatcatta tggcttcgac cggatcattc
    19261 agcgcaatgg cgattttctg gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg
    19321 atcggtattg cggtgatcaa cgccactggc aacattggtt cagcgttaag tccgtttatg
    19381 atcggctggt tgaaagattt gaccggcagc tttaacagtg gattgtggtt tgttgccgcg
    19441 ctgctggtga ttggtgcggg gattatctgg gcgattccaa tgcagtcctc ccgtccgcga
    19501 gcgaccccgt aaggaacgac gatgtgtgac cgtcagattg ccaatattga tatcagcaaa
    19561 gagtacgatg aaagcctggg cacggacgat gtgcattatc agtccttcgc ccgcatggcg
    19621 gccttttttg gccgccatat gctgccacat cgccacgaac agtactttca gatgcatttc
    19681 ctcaatagcg gacagattga gctacaactt gacgatcatc gctactcggt ggaagcgccc
    19741 ctgtttgtcc tgacgccgcc gtcagtacct catgcgttta ttacggagtc tgacgccgac
    19801 ggtcatgtgt tgacggtacg ggaagatctg atctggcccc tgctggaagt tctttatccg
    19861 ggcactaggg aaaccttcgg cctgccgggg atttgcctgt cactggcaga taaacccgac
    19921 gaactggcgg cgctggaaca ctattggcaa ctgatagagc gggaatcggt ggaacaactg
    19981 cctggacggg aacacaccct gacgttactg gcacaggcag tgttcaccct actgctgcgt
    20041 aacgcaaaac tcgacgacca tgccgccagc gggatgcgcg gagaattaaa actgttccag
    20101 cgttttaata tgctgattga aagccatttt catcagcact ggacagtacc ggattacgct
    20161 aacgaactgc atatcaccga atcacgactc acggacatct gccgtcgctt tgccaaccgt
    20221 ccgccaaaac ggttgatttt cgacaggcag ctgcgagaag ccaagcggct gctgctgttt
    20281 tctgataacg ccgtgaacaa tattgcctgg caactcggtt ttaaggatcc ggcttatttt
    20341 gcgcgctttt ttaatcgctt agtcggttgc tcgcccagtg cttatcgcgc caaaaaagta
    20401 cctgtgacgt gaaaatccct tagccttaac gggaaaccag gcaccacctg ctattcccct
    20461 ttctccgcct ggagcaaggg gatttagtaa cgtcaaaaat cgctaaaagc gacctcgatc
    20521 acaaaaccgt acccgacctg aaaagtacca gccattgtcc caaaagtgtc ttcccgtaat
    20581 cgcccctgca agcttcaatt gaataacaaa aacaacacac aaaattaaca atcgtattcc
    20641 gattaatact gtagaggtcg acatgaaacc agaagatttc cgcgccagta cccaacgtcc
    20701 gttcaccggg gaagagtatc tgaaaagcct gcaggatggt cgcgagatct atatctatgg
    20761 cgagcgagtg aaagacgtca ccactcatcc ggcatttcgt aatgcggcag cgtctgttgc
    20821 ccagctgtac gacgcactgc acaaaccgga gatgcaggac tctctgtgct ggaacaccga
    20881 caccggcagc ggcggctata cccataaatt cttccgcgtg gcgaaaagtg ccgacgacct
    20941 gcgccagcaa cgcgacgcca tcgctgagtg gtcacgcctg agctatggct ggatgggccg
    21001 taccccagac tacaaagctg ctttcggttg cgcactgggc gcgaatccgg gcttttacgg
    21061 tcagttcgag cagaacgccc gtaactggta cacccgtatt caggaaactg gcctctactt
    21121 taaccacgcg attgttaacc caccgatcga tcgtcatttg ccgaccgata aagtaaaaga
    21181 cgtttacatc aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt
    21241 ggttgccacc aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt
    21301 aatgggcgaa aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt
    21361 gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt gctaccggct caccgtatga
    21421 ctacccgctc tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtgct
    21481 gatcccatgg gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat
    21541 ggaaggcggt ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact
    21601 cgacttcatt acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg
    21661 tggtgtgcaa gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag
    21721 tgactcgatg tgttctgaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc
    21781 cgcactgcaa acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat
    21841 cgaacgcaac gttaccagtg gcctgatcta tctcccttcc agtgcccgtg acctgaacaa
    21901 tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt
    21961 ccagcgcatc aagatcctca aactgatgtg ggatgccatt ggcagcgagt ttggtggtcg
    22021 tcacgaactg tatgaaatca actactccgg tagccaggat gagattcgcc tgcagtgtct
    22081 gcgccaggca caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct
    22141 gtcggaatac gaccagaacg gctggactgt gcctcacctg cacaacaacg acgatatcaa
    22201 catgctggat aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa
    22261 cgcctgcgct ttcgtgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc
    22321 gagggcgacg ccggacaatg cgggattacg gcaacggccg tctgctcggt cacggataca
    22381 ccaccatcgc tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc
    22441 aacggcaagt tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc acgccacttc
    22501 gcgggcatga cgggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt
    22561 ccgctggcgc agccggtgct aaaaggttcg ctggccagtc ttgaaggtga gatccgcgat
    22621 gtgcaggcaa ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt
    22681 gcagaaggtc acggacttat ctactttaaa cgccgtttcc atccggtgat gctggaaatg
    22741 gaagctgcga tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg
    22801 atctcaatca catatccacc cgatgaataa ttcttgccca accgctcacc gccattcctg
    22861 accgcttaca tccctaaaat aaccactcag ttatttacct tactttacgc gcgcgtatct
    22921 ctggcaacat cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag
    22981 aacggcttcg gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc
    23041 aagtgagtga atccccggga gcttgcaaca gtaagtgaca ggggtgaacg aacgcagctg
    23101 ccgcacctgt aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa
    23161 aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
    23221 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
    23281 cggtgtatct ggtggcgtat cgctactaca gtttgtatat cgcccagaag gtgatgaaac
    23341 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
    23401 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
    23461 ttggtccggt tctcgccgcg cagatgggct acctacctgg cacgctgtgg ctgctggcgg
    23521 gggtagtact ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta
    23581 acggcgcatc tctcggtgag atgatcaaag aagagatggg accagtaccg gggactatcg
    23641 cgctgtttgg ctgtttctta atcatgatca tcatccttgc cgtcctggcg ctgattgtgg
    23701 ttaaagccct ggccgaaagc ccgtggggtg ttttcaccgt ttgctcaacc gtaccgattg
    23761 cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg
    23821 tcattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    23881 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    23941 atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg
    24001 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtattgtg gtgctgaacc
    24061 cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag
    24121 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    24181 cgctgatctc ttccggtacg actccgaaac tgctggctaa cgaaaccgac gcgcgtttca
    24241 ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gtcgctgcgt
    24301 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatta
    24361 ccatgcctaa cctgcatgaa atgggtggcg agaatgcgcc gatcatcatg gcgcagctga
    24421 aagacgttac cgcacacgcg gcggcaaccg tcagctcctg gggcttcgtg atttcgccag
    24481 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    24541 gtgcgccaac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    24601 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
    24661 tggatgcggg tactcgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    24721 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    24781 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    24841 tgtggccgct gttcggtatt tccaaccaga tgctggcagc cgtagcgctg gtactgggca
    24901 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
    24961 tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc
    25021 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    25081 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    25141 caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    25201 cctggctgaa ggtgcgtaac agcgacaaac gtactgacaa agaaactccg tacgttccaa
    25261 tcccggaagg cggcgtgaag atctcttcgc accactaacc ggttgccgga tgcgctgcgc
    25321 gtatccggcc tacaaagtgt ctatcgtagg ccggataaag cgtttacgcc gcatccggca
    25381 aatccctagc ccggaccttc cgggctttac gcatatctgg aaggcaaaat gtttggtaac
    25441 ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg cattccagac
    25501 tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat
    25561 gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg
    25621 cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt
    25681 aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg gctacaagat
    25741 tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga ttggcgaccg
    25801 cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct
    25861 ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgaccg
    25921 tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga cctttttctc
    25981 ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg tggatgcggt
    26041 acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg gctacgccga
    26101 ccgtattctg ctgactaaaa ccgacgtcgc aggcgaagca gagaaactgc gtgaacgcct
    26161 ggcgcgcatc aacgcccgtg caccggtcta caccgtcacc cacggcgata tcgacctggg
    26221 cctgctattc aacaccaacg gttttatgct ggaagagaac gtggtcagca ccaagccacg
    26281 tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg aactggatta
    26341 cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg aatcggcgga
    26401 taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctgtt
    26461 ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg atgaaacacc
    26521 gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc gggcggcgtt
    26581 tgcggggttg aggaagtaat cgtgaaaggt tgagtgtaaa gcagctcaat gccccattta
    26641 gcagtgaggg gggaaacaga acgacgtgct cccctccgct aagttttatt gcgcgtgaat
    26701 acctggatct ggcaaaaaat tgccgcgttt ggcgtcagac catgcttcgc taccagaagc
    26761 aggaatgtcg ctgtaatcaa tgtcttcgtc ggatttatta accaaagcca tgagttcagc
    26821 ttcttgctgc acatcaagtg tgtagagtgt gccccgttta tgtttaacca tgctcattta
    26881 tgaccagaac ctttacgatt taattataaa gatgacttat ttcagccatc caggcacgtt
    26941 atattctgag acattctcaa tggaatatgc atctctcctg tcagaacctt tcttacactt
    27001 caactcattg aacccccatt t
//
LOCUS       DAPHDX010000058        26723 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPHDX010000058 DAPHDX010000000
VERSION     DAPHDX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.058"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..264
                     /gene="tuf"
                     /locus_tag="RH058_002360"
     CDS             <1..264
                     /gene="tuf"
                     /locus_tag="RH058_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3137940.1"
                     /translation="EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMV
                     MPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            447..641
                     /gene="bfd"
                     /locus_tag="RH058_002361"
     CDS             447..641
                     /gene="bfd"
                     /locus_tag="RH058_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV3137941.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            714..1190
                     /gene="bfr"
                     /locus_tag="RH058_002362"
     CDS             714..1190
                     /gene="bfr"
                     /locus_tag="RH058_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV3137942.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1187..1654)
                     /locus_tag="RH058_002363"
     CDS             complement(1187..1654)
                     /locus_tag="RH058_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV3137943.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2033..2344
                     /gene="rpsJ"
                     /locus_tag="RH058_002364"
     CDS             2033..2344
                     /gene="rpsJ"
                     /locus_tag="RH058_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV3137944.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2377..3006
                     /gene="rplC"
                     /locus_tag="RH058_002365"
     CDS             2377..3006
                     /gene="rplC"
                     /locus_tag="RH058_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV3137945.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3017..3622
                     /gene="rplD"
                     /locus_tag="RH058_002366"
     CDS             3017..3622
                     /gene="rplD"
                     /locus_tag="RH058_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV3137946.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3619..3921
                     /gene="rplW"
                     /locus_tag="RH058_002367"
     CDS             3619..3921
                     /gene="rplW"
                     /locus_tag="RH058_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV3137947.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3939..4760
                     /gene="rplB"
                     /locus_tag="RH058_002368"
     CDS             3939..4760
                     /gene="rplB"
                     /locus_tag="RH058_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV3137948.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4777..5055
                     /gene="rpsS"
                     /locus_tag="RH058_002369"
     CDS             4777..5055
                     /gene="rpsS"
                     /locus_tag="RH058_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV3137949.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5070..5402
                     /gene="rplV"
                     /locus_tag="RH058_002370"
     CDS             5070..5402
                     /gene="rplV"
                     /locus_tag="RH058_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV3137950.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5420..6121
                     /gene="rpsC"
                     /locus_tag="RH058_002371"
     CDS             5420..6121
                     /gene="rpsC"
                     /locus_tag="RH058_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV3137951.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6134..6544
                     /gene="rplP"
                     /locus_tag="RH058_002372"
     CDS             6134..6544
                     /gene="rplP"
                     /locus_tag="RH058_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV3137952.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6544..6735
                     /gene="rpmC"
                     /locus_tag="RH058_002373"
     CDS             6544..6735
                     /gene="rpmC"
                     /locus_tag="RH058_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV3137953.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6735..6989
                     /gene="rpsQ"
                     /locus_tag="RH058_002374"
     CDS             6735..6989
                     /gene="rpsQ"
                     /locus_tag="RH058_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV3137954.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7154..7525
                     /gene="rplN"
                     /locus_tag="RH058_002375"
     CDS             7154..7525
                     /gene="rplN"
                     /locus_tag="RH058_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV3137955.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7536..7850
                     /gene="rplX"
                     /locus_tag="RH058_002376"
     CDS             7536..7850
                     /gene="rplX"
                     /locus_tag="RH058_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV3137956.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7865..8404
                     /gene="rplE"
                     /locus_tag="RH058_002377"
     CDS             7865..8404
                     /gene="rplE"
                     /locus_tag="RH058_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV3137957.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8419..8724
                     /gene="rpsN"
                     /locus_tag="RH058_002378"
     CDS             8419..8724
                     /gene="rpsN"
                     /locus_tag="RH058_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV3137958.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8758..9150
                     /gene="rpsH"
                     /locus_tag="RH058_002379"
     CDS             8758..9150
                     /gene="rpsH"
                     /locus_tag="RH058_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV3137959.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9163..9696
                     /gene="rplF"
                     /locus_tag="RH058_002380"
     CDS             9163..9696
                     /gene="rplF"
                     /locus_tag="RH058_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV3137960.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9706..10059
                     /gene="rplR"
                     /locus_tag="RH058_002381"
     CDS             9706..10059
                     /gene="rplR"
                     /locus_tag="RH058_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV3137961.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10074..10577
                     /gene="rpsE"
                     /locus_tag="RH058_002382"
     CDS             10074..10577
                     /gene="rpsE"
                     /locus_tag="RH058_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV3137962.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10581..10760
                     /gene="rpmD"
                     /locus_tag="RH058_002383"
     CDS             10581..10760
                     /gene="rpmD"
                     /locus_tag="RH058_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV3137963.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10764..11198
                     /gene="rplO"
                     /locus_tag="RH058_002384"
     CDS             10764..11198
                     /gene="rplO"
                     /locus_tag="RH058_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV3137964.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11206..12537
                     /gene="secY"
                     /locus_tag="RH058_002385"
     CDS             11206..12537
                     /gene="secY"
                     /locus_tag="RH058_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV3137965.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12569..12685
                     /gene="rpmJ"
                     /locus_tag="RH058_002386"
     CDS             12569..12685
                     /gene="rpmJ"
                     /locus_tag="RH058_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV3137966.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12832..13188
                     /gene="rpsM"
                     /locus_tag="RH058_002387"
     CDS             12832..13188
                     /gene="rpsM"
                     /locus_tag="RH058_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV3137967.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13205..13594
                     /gene="rpsK"
                     /locus_tag="RH058_002388"
     CDS             13205..13594
                     /gene="rpsK"
                     /locus_tag="RH058_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV3137968.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13628..14248
                     /gene="rpsD"
                     /locus_tag="RH058_002389"
     CDS             13628..14248
                     /gene="rpsD"
                     /locus_tag="RH058_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV3137969.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14274..15263
                     /gene="rpoA"
                     /locus_tag="RH058_002390"
     CDS             14274..15263
                     /gene="rpoA"
                     /locus_tag="RH058_002390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV3137970.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15304..15687
                     /gene="rplQ"
                     /locus_tag="RH058_002391"
     CDS             15304..15687
                     /gene="rplQ"
                     /locus_tag="RH058_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV3137971.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15794..16162
                     /locus_tag="RH058_002392"
     CDS             15794..16162
                     /locus_tag="RH058_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV3137972.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16173..16598
                     /gene="zntR"
                     /locus_tag="RH058_002393"
     CDS             16173..16598
                     /gene="zntR"
                     /locus_tag="RH058_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV3137973.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16654..16872
                     /gene="arfA"
                     /locus_tag="RH058_002394"
     CDS             16654..16872
                     /gene="arfA"
                     /locus_tag="RH058_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV3137974.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16869..17279)
                     /gene="mscL"
                     /locus_tag="RH058_002395"
     CDS             complement(16869..17279)
                     /gene="mscL"
                     /locus_tag="RH058_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV3137975.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17409..18785)
                     /gene="trkA"
                     /locus_tag="RH058_002396"
     CDS             complement(17409..18785)
                     /gene="trkA"
                     /locus_tag="RH058_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV3137976.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18807..20096)
                     /gene="rsmB"
                     /locus_tag="RH058_002397"
     CDS             complement(18807..20096)
                     /gene="rsmB"
                     /locus_tag="RH058_002397"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV3137977.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20142..21089)
                     /gene="fmt"
                     /locus_tag="RH058_002398"
     CDS             complement(20142..21089)
                     /gene="fmt"
                     /locus_tag="RH058_002398"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV3137978.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21104..21613)
                     /gene="def"
                     /locus_tag="RH058_002399"
     CDS             complement(21104..21613)
                     /gene="def"
                     /locus_tag="RH058_002399"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV3137979.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21743..22867
                     /gene="dprA"
                     /locus_tag="RH058_002400"
     CDS             21743..22867
                     /gene="dprA"
                     /locus_tag="RH058_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV3137980.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22839..23312
                     /locus_tag="RH058_002401"
     CDS             22839..23312
                     /locus_tag="RH058_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV3137981.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23341..23883
                     /locus_tag="RH058_002402"
     CDS             23341..23883
                     /locus_tag="RH058_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV3137982.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23888..24460
                     /gene="tsaC"
                     /locus_tag="RH058_002403"
     CDS             23888..24460
                     /gene="tsaC"
                     /locus_tag="RH058_002403"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV3137983.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24465..25283
                     /gene="aroE"
                     /locus_tag="RH058_002404"
     CDS             24465..25283
                     /gene="aroE"
                     /locus_tag="RH058_002404"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV3137984.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25280..25537
                     /locus_tag="RH058_002405"
     CDS             25280..25537
                     /locus_tag="RH058_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV3137985.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25513..26067)
                     /locus_tag="RH058_002406"
     CDS             complement(25513..26067)
                     /locus_tag="RH058_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV3137986.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26540..>26723
                     /locus_tag="RH058_002407"
     rRNA            26540..>26723
                     /locus_tag="RH058_002407"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6880 a         6331 c         6930 g         6582 t
ORIGIN      
        1 gaagtgtaca ttctgtccaa agatgaaggc ggccgtcata ctccgttctt caaaggctac
       61 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggc
      121 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      181 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      241 gttgttgcta aagttctggg ctaattacac gttaattagt tttgaattga aaagggcgct
      301 tcggcgccct ttttgctgcc cgtaatttga gctcgaaata tttagtcgta attttgctcg
      361 ctgaactatg gcgcaaataa aactgtgcta ttgtaatcat aaccattctc atttacactt
      421 tgcgcagaat ttgaacggga gcaatcatgt acgtttgttt gtgtaatggt gtaagtgaca
      481 aaaaaattcg ccaggcagtc cgtcagtttc atccgcaatc tttccaacaa ttgcgtaagt
      541 ttattcctgt gggaaatcaa tgcggtaagt gcattcgcgc cgcgcgcgaa gtgatgcagg
      601 acgagttgat gcagatgcca gaatttaaag agattgcctg aagaccacgc ttattttttg
      661 acatcccgct agccccatct acgctctaat agtggaagcg gagggactat aaaatgaaag
      721 gtgatgttaa aatcataaat tatctcaata aactattggg aaatgagctt gtcgcaatca
      781 atcagtattt tctccatgcc cggatgttta aaaactgggg cctgatgcgt ctcaatgacg
      841 tcgaatacca tgaatccatc gatgagatga aacacgctga taaatatatc gagcgtattt
      901 tgtttttaga aggaatcccc aacttacagg atttaggcaa actggggatt ggtgaagatg
      961 tcgaggagat gctgcaatcc gacctgcgat tagaactgga aggcgcgaag gatctccgtg
     1021 aggctatcgc gtatgccgac agcgttcatg actatgttag ccgtgatatg atgatcgaga
     1081 tccttgccga tgaagaaggt catattgact ggctcgaaac tgagctggat ctcatcggta
     1141 aaatcggttt acaaaattac ctgcaatcac aaataaaagt taaagattaa cggcctgcca
     1201 gcaaactatc ccaacctaca acgaaacccg cagccgccag aaatggccca aacggcagcg
     1261 ggttttttaa tgactctttt cctctcatga gcagaccaac aactacggct ccgcaagcga
     1321 atgaggcagc aaggaaaacc agtcgtggca agaaggtcca ggtatgccag gcaccaaggg
     1381 cggcgagaaa cttcacatca ccatagccta atccttcttt atgacgcagt atgcgatacc
     1441 cccagtaaat gacagcgaat gtgccgtaac caataatcgc cccccataac gcatcggcta
     1501 aaccgtcagg attacaaacc tgagagaaca aaagacctga ccagagtaac gggcaggtaa
     1561 atctgtcagg taacaagcca tgctttgcat cccataaaaa aagtaggaca gataaacaag
     1621 cgtacaaaat cagaaaggga agggtagcgt ccataaaaca tcctctgtaa ttggagtatc
     1681 accatcgaat aactccggat gctcagcaac agagaaatcg ctgatttgtt aacctggttc
     1741 cggtgaaaaa cgctatcttc acaaccttac aaatttcgtg gaatacagct cgcaatctgc
     1801 gcgttgcgat taatttctac ccgatgcttg cgtcaggctc agatttgcgt ataatgcgcg
     1861 ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct
     1921 aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat
     1981 gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa
     2041 ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc
     2101 ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc
     2161 gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga
     2221 tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac
     2281 cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg
     2341 ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa
     2401 gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa
     2461 gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt
     2521 caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc
     2581 gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa
     2641 gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac
     2701 gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt
     2761 acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag
     2821 aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa
     2881 cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg
     2941 gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag
     3001 gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc
     3061 cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta
     3121 tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc
     3181 cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag
     3241 cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa
     3301 agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca
     3361 ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc
     3421 acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga
     3481 aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat
     3541 cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca
     3601 agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg
     3661 cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt actcaaagtt
     3721 gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa
     3781 gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc
     3841 ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac
     3901 ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac
     3961 cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca
     4021 aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc
     4081 gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca
     4141 aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt
     4201 ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta
     4261 aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta
     4321 acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac
     4381 caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg
     4441 atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact
     4501 gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag
     4561 caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg
     4621 tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt
     4681 ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg
     4741 tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt
     4801 ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag
     4861 aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc
     4921 atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt
     4981 cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa
     5041 gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc
     5101 gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc
     5161 aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc
     5221 tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag
     5281 ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag
     5341 gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct
     5401 gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat
     5461 tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga
     5521 cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg
     5581 tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg
     5641 tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat
     5701 cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa
     5761 actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat
     5821 gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag
     5881 cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc
     5941 gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg
     6001 tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt
     6061 tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata
     6121 aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg
     6181 taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt
     6241 tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc
     6301 agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa
     6361 gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat
     6421 tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc
     6481 attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat
     6541 gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg
     6601 aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag
     6661 tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag
     6721 aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca
     6781 aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta
     6841 aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg
     6901 gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg
     6961 ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg
     7021 ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc
     7081 gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta
     7141 gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc
     7201 acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg
     7261 cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga
     7321 tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt
     7381 cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg
     7441 tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat
     7501 ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg
     7561 acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc
     7621 tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg
     7681 ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca
     7741 acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag
     7801 acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag
     7861 tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga
     7921 gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg
     7981 tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc
     8041 aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat
     8101 ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt
     8161 ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc
     8221 taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc
     8281 agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac
     8341 tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa
     8401 gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta
     8461 gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg
     8521 aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat
     8581 tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg
     8641 cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg
     8701 ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg
     8761 agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac
     8821 aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag
     8881 gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt
     8941 actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca
     9001 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
     9061 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
     9121 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
     9181 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
     9241 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
     9301 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
     9361 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
     9421 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct
     9481 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
     9541 actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg
     9601 gatctgcgcg cctaccgtcg tcctgagcct tataaaggca agggtgttcg ttacgccgac
     9661 gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
     9721 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
     9781 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
     9841 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
     9901 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
     9961 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
    10021 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
    10081 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
    10141 aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta
    10201 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
    10261 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctgcaac
    10321 accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct tccgaaggta
    10381 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
    10441 ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg
    10501 gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
    10561 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
    10621 tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc
    10681 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
    10741 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
    10801 aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
    10861 gtggtcacaa aggtcagaaa tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
    10921 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
    10981 ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga
    11041 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
    11101 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
    11161 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
    11221 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
    11281 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
    11341 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac
    11401 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat
    11461 atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt ggcagaaatt
    11521 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgtta cggtactctg
    11581 gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg
    11641 caaggcctgg tgattaaccc aggctttgca ttctacttca ctgctgttgt aagtctggtc
    11701 acaggaacga tgttcctgat gtggttgggc gaacagatta ctgaacgagg tatcggcaac
    11761 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
    11821 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
    11881 ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg
    11941 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
    12001 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
    12061 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
    12121 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
    12181 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
    12241 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
    12301 atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac ctttatctgc
    12361 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
    12421 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
    12481 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
    12541 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg
    12601 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
    12661 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
    12721 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg
    12781 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
    12841 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
    12901 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
    12961 aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt
    13021 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
    13081 ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
    13141 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
    13201 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
    13261 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
    13321 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
    13381 tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat
    13441 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
    13501 tctgaacgcc gcaggtttcc gcatcactaa tattactgat gtgactccga tccctcataa
    13561 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag
    13621 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
    13681 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
    13741 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
    13801 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
    13861 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
    13921 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
    13981 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
    14041 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
    14101 tctggagctg gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat
    14161 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
    14221 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
    14281 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
    14341 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
    14401 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
    14461 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
    14521 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
    14581 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa
    14641 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
    14701 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
    14761 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
    14821 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca
    14881 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
    14941 ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga
    15001 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
    15061 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
    15121 aacgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
    15181 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac
    15241 cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag
    15301 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
    15361 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
    15421 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
    15481 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
    15541 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
    15601 tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca
    15661 gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg
    15721 gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc
    15781 ccctggagta tatatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca
    15841 agcgaaaggt gagtttgata acctggcagg tagcggcgaa ccattgatac tggatgatga
    15901 ttctcacgtg ccaccggaat tacgtgcggg gtatcgcttg ttgaagaatg ccggttgctt
    15961 accgccagaa cttgagcaac ggagggaagc aattcagctt ctggatattc tcaaaggtat
    16021 ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa
    16081 gctgcgacaa gctggactga gtaccgattt tttacgcggc gattatgctg acaagttgtt
    16141 gaataaaatc aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa
    16201 tggcggaagt aacacccgac acgattcgtt attacgaaaa acagcagatg atggagcatg
    16261 aagtgcgtac tgaaggtggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat
    16321 ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga
    16381 tccgtatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat
    16441 tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc
    16501 ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc
    16561 ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg acaggattac
    16621 actcgcgccg ttaaataacc aactggagtt tttatgagcc gatatcagca tactaaaggg
    16681 cagataaagg ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgagta
    16741 gagaaaaata agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg
    16801 aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggcct tctgctttca
    16861 ggcgtttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat
    16921 acttcttctt tagttggtgc tggtgcggct gctggttctt cttttttccg attcagtttg
    16981 ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc aaagacgttt
    17041 tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccctgcgc atcgcgtagc
    17101 gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg
    17161 atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc
    17221 gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg
    17281 ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca ttttcctgcc
    17341 aggaatgaat ttaattaatc cttgataatc aaagggaaat aataaggcgt cattagacgc
    17401 cttattaatt acaagaagaa agggcttggc tggaagagtc tttcgacgtc ggtaataaac
    17461 tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg
    17521 gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg
    17581 atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct
    17641 tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc tgcttttcgc
    17701 acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaatcgcaat atcgatcacg
    17761 ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca
    17821 cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca
    17881 ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca
    17941 ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta
    18001 cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata
    18061 ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc
    18121 atcaccgcac ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttccaca
    18181 atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta
    18241 tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcgt taccaatcag cgggccgcca
    18301 taataggctt taaccacagc gaggctgact ttaccctcag cgaagttcac cacctgcaat
    18361 gcgccgggat actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc
    18421 agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg cacgtagtct
    18481 ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag
    18541 gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca
    18601 cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat
    18661 ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtgat atcgttgttc
    18721 tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt
    18781 ttcatcagtc gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg
    18841 tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg
    18901 caaagttcgg catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc
    18961 ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg
    19021 gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac
    19081 catttaatat ccggatggcg acgaatcaca ccggttgctg aacaaggcgc atctaataaa
    19141 atgcgatcaa actgttgctc gccacaccat tgggaagggt agcggccatc accttgtttc
    19201 acggtcgctt tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg
    19261 tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta
    19321 ccgccggggg cggcacaaag atccagaatg tgttcaccgt tttgtggcgc aagccaggtc
    19381 atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc
    19441 gcatgaacag gtgcaggtgt ttccagccgt acagcatcag ggtaatccgc atgcgggaaa
    19501 cctttcattc ctgcttcatc caacaatgca agccagctgt cgcgggaatg atgcgtacga
    19561 ttaacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc
    19621 tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca
    19681 ctggcattaa actcggctaa taactcttct tgctgacgct ggaactggcg taatacaccg
    19741 tttatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac cgtttcagcc
    19801 agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg
    19861 taatgcacgg tccgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag
    19921 tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct
    19981 ttatcggaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg
    20041 acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt
    20101 ataaaaacgc ccggcaagac cgggcttaga agagtggact atcagaccag acggttgccc
    20161 ggaacaaacc attcccgacg agagttcagg aggtcttgcg cgctcatcgc tttcttaccc
    20221 gcaggttgta acgagagcag gttcaggatg ccatcaccag tcgcaacctg aatgccttgt
    20281 ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgatgct
    20341 ttccagactt taaccggctg tccttcaatt tccagccagc tcattggcca tggattgaaa
    20401 gcgcgaatgc agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct
    20461 ttactcaact tttcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg
    20521 ccgtctgcca gttgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg
    20581 tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt atagagcatg
    20641 tcaccggtgt ctaaaccgac atccatttgc ataatggtca caccagtttc tgcatcaccc
    20701 gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga
    20761 acgttgatac agccaagacg cggcatctcc agcactgctt taggcagaat taagccatag
    20821 gcgacgacga ccataacatc agcctgcaga tcggcgacca gttgctggtt ttcttgtgga
    20881 cgcagggaaa caggttgaaa aacgggcaga cctttttcct cagccagaac tttaaccggg
    20941 ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg
    21001 acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc
    21061 gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca
    21121 gacgatccag tttttcaact ttctgacgaa tacgttgttg tttcaacggt gacagataat
    21181 ccataaacag tttgccgacc aggtgatcca tctcatgctg aatacagatg gctaacagac
    21241 cgtctgcttc caattcaaat ggtttaccgt cgcggtccag ggcgcgaatt ttaactttct
    21301 ctgcgcgtgg cactaaagca cgttgttcag ggatcgacag gcaaccttct tcaatgcctg
    21361 tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt
    21421 ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc gccaggccaa
    21481 taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat
    21541 tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt
    21601 gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat
    21661 tctagacaaa tccccctctg attgacagca tcactgacca atcgcaaaga ttgctaaggc
    21721 tgcttatggc agggagataa ggatggtcga tacagaaatt tggctgcgtt taatgagtat
    21781 cagcagcttg tacggcgatg atatggtccg tatagctcac tggctggcaa aacagtcgca
    21841 aattgatgcg gttggattgc agcaaacagg gcttacattg cggcaggcac aacgctttct
    21901 ttcatttccg cgaaagagta tcgaaagctc actttgttgg ttggagcaac ccaaccatca
    21961 tttaatccct gcggacagcg aattttatcc tcctcaactt caggcgacga cagattaccc
    22021 cggcgcactg tttgttgaag gagaactgca cgcgctgcat tcatttcagc ttgccatagt
    22081 ggggagtcgg gcgcattcat ggtatggcga gcgatgggga cgattatttt gcgaaactct
    22141 ggcgacgcgt ggagtgacaa ttacaagtgg actggcgcgt ggaatcgatg gtgtggcgca
    22201 taaagcggcc ttacaggtaa atggcgtcag cattgctgta ttggggaatg gacttaatac
    22261 cattcatccc cgccgccatg cccgactggc tgccagtttg cttgaacatg gcggagctct
    22321 cgtctcggaa tttcccctcg atgttccacc ccttgcttac aatttcccac gaagaaatcg
    22381 cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc gcagtggttc
    22441 gctagtgaca gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct tgccaggtcc
    22501 aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg cgattcttgt
    22561 gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt tgccagacgc
    22621 ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat ttcctgagct
    22681 cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca
    22741 acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat ggatcgcagc
    22801 tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta ctaatgtatt
    22861 tgtttgaaac ctatattcac acagaagctg agttgcgtgt ggatcaagac aaacttgaac
    22921 aggatcttac cgacgcagga tttgatcgag aagatattta caatgccctg ctttggctgg
    22981 aaaagcttgc ggattatcag gaaggactgg cagaaccgat gcaactggcc tctgatccac
    23041 tctccatgcg tatttatacc cctgaggagt gtgaaagact ggatgccagc tgtcgtggtt
    23101 ttctgctttt ccttgagcag attcaggtgc tcaaccttga aactcgtgaa atggtgatag
    23161 agcgagtgct cgcgctggat aacgcagagt tcgaactgga tgatctgaaa tgggtaatcc
    23221 tgatggtgtt gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg gaagaattac
    23281 tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc ataagttgtt
    23341 atggcgaaat cagcactgtt cacggtgcgt aataatgagt cctgcccaaa gtgcggggct
    23401 gaactggtta ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca gtatccggcg
    23461 tgtgactacg tccgtcctct gaaatcttca gcggatggac atattgtcaa agttctggag
    23521 gggcaggttt gccctgcatg tggcgaaaat ctggtattac gccagggacg ctttggtatg
    23581 tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga taaaccggac
    23641 gaaacagcaa ttacatgccc ccaatgccgg acgggccatc tggtccagcg ccgctcccgt
    23701 tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc cattaacttc
    23761 aaacccatag ctggagaatg ccctgagtgt cattatccac tactcatcga aaagaaaacc
    23821 gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa
    23881 taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa
    23941 tgaagaacgt gtcatcgcct atccaacgga agccgttttc ggtgtcgggt gcgatcctga
    24001 tagcgaaaca gcagtgatgc gactgttgga gttaaaacag cgtccggttg ataaggggct
    24061 gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca ccatgctgac
    24121 tgatgcgcag cgtgaaacca ttttttcccg ctggccaggt cctgtcacct ttgtctttcc
    24181 cgcgcctgcg acaacaccgc gctggttgac gggccgcttc gattcgcttg ctgtacgagt
    24241 caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac
    24301 cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc gcgcacaatt
    24361 tggcgcggcg ttcccggttg tgcctggtga aacggggggg cgtttaaatc cttcagaaat
    24421 ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa acctatgctg
    24481 tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa tttgctcagc
    24541 aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat ttcatcaaca
    24601 cactgaacgc tttctttcgt gctggtggta aaggtgcgaa tgtgacggtg ccttttaaag
    24661 aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct ggtgctgtta
    24721 ataccctcaa gcggttagaa gatggacgcc tgctgggtga caataccgat ggtataggtt
    24781 tgttaagcga tctggaacgt ctgtctttta tccgtcctgg tttacgtatt ctgctcatcg
    24841 gcgctggtgg agcatctcgc ggcgtactac tgccacttct ttccctggac tgtgcggtga
    24901 caatcactaa tcggacggta tcccgcgcgg aagagttggc taaattgttt gcgcacactg
    24961 gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat ctcattatta
    25021 atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg ctcattcatc
    25081 caggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt ctggcatggt
    25141 gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg gcacaggcgg
    25201 ctcatgcctt tcttctctgg cacggtgttc tgcctgacgt agaaccagtt ataaagcaat
    25261 tgcaggagga attgtccgcg tgaatcaggc catccagttt ccggacagag aagagtggga
    25321 cgagaataaa aaatgtgtat gttttcccgc tctcgtgaat ggcatgcaac tgacatgtgc
    25381 gatctctggc gagagtctgg cgtatcgttt tactggagat acgccagaac agtggttagc
    25441 gagttttcgt cagcatcgct gggatctgga agaagaagcg gaaaacttaa ttcaggaaca
    25501 aagtgaagat gatcaaggct gggtctggtt accctgatcc agatattcgt ccttccattt
    25561 cacgtaatta ttcgcggaat agcgtaaccc agccttctct tcatcactta acgggcggat
    25621 ctgtttgacg gggctaccga gatacagata tccgctctcc agccgtttat tttgtgggac
    25681 cagactaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa gtaaaattga
    25741 gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca ccttgtgacc
    25801 aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg aggatttatg
    25861 agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct gtacataatg
    25921 tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca gacgaacgtc
    25981 accaatcacg acactgctat cgtcgatcat tacgcgctga ccgatttgtg gaaaaagatc
    26041 gcggtatggg cgtaaaacat cagacatact tacctcagca ataaatgatt tactaatgac
    26101 tttgggggca ttattggcct tgtgcaagtc ttttagtatg caaaaaagca ccgttttgtg
    26161 tgcgaatgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca aaaaagtact
    26221 tgtgcaaaaa attgggatcc ctataatgcg cctccgttga gacgacaacg tgaaacactt
    26281 cacaagatgg tcgtaacaac gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    26341 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    26401 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    26461 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
    26521 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    26581 gcaggcctaa cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga
    26641 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
    26701 acggtagcta ataccgcata acg
//
LOCUS       DAPHDX010000059        26105 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPHDX010000059 DAPHDX010000000
VERSION     DAPHDX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.059"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..1788)
                     /locus_tag="RH058_002408"
                     /pseudo
     CDS             complement(1..1788)
                     /locus_tag="RH058_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2312..4240
                     /gene="thrS"
                     /locus_tag="RH058_002409"
     CDS             2312..4240
                     /gene="thrS"
                     /locus_tag="RH058_002409"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV3137987.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4244..4786
                     /gene="infC"
                     /locus_tag="RH058_002410"
     CDS             4244..4786
                     /gene="infC"
                     /locus_tag="RH058_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV3137988.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4883..5080
                     /gene="rpmI"
                     /locus_tag="RH058_002411"
     CDS             4883..5080
                     /gene="rpmI"
                     /locus_tag="RH058_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV3137989.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5133..5489
                     /gene="rplT"
                     /locus_tag="RH058_002412"
     CDS             5133..5489
                     /gene="rplT"
                     /locus_tag="RH058_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV3137990.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5606..5730
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5612..5656
                     /gene="pheM"
                     /locus_tag="RH058_002413"
     CDS             5612..5656
                     /gene="pheM"
                     /locus_tag="RH058_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV3137991.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5940..6923
                     /gene="pheS"
                     /locus_tag="RH058_002414"
     CDS             5940..6923
                     /gene="pheS"
                     /locus_tag="RH058_002414"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV3137992.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6938..9325
                     /gene="pheT"
                     /locus_tag="RH058_002415"
     CDS             6938..9325
                     /gene="pheT"
                     /locus_tag="RH058_002415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV3137993.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9330..9629
                     /gene="ihfA"
                     /locus_tag="RH058_002416"
     CDS             9330..9629
                     /gene="ihfA"
                     /locus_tag="RH058_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV3137994.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9730..10710
                     /gene="btuC"
                     /locus_tag="RH058_002417"
     CDS             9730..10710
                     /gene="btuC"
                     /locus_tag="RH058_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV3137995.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            10773..11324
                     /gene="btuE"
                     /locus_tag="RH058_002418"
     CDS             10773..11324
                     /gene="btuE"
                     /locus_tag="RH058_002418"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV3137996.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11324..12073
                     /gene="btuD"
                     /locus_tag="RH058_002419"
     CDS             11324..12073
                     /gene="btuD"
                     /locus_tag="RH058_002419"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV3137997.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12151..12615
                     /locus_tag="RH058_002420"
     CDS             12151..12615
                     /locus_tag="RH058_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3137998.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12862..13575
                     /gene="ydiV"
                     /locus_tag="RH058_002421"
     CDS             12862..13575
                     /gene="ydiV"
                     /locus_tag="RH058_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV3137999.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13638..15074
                     /locus_tag="RH058_002422"
     CDS             13638..15074
                     /locus_tag="RH058_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV3138000.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLHRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15078..15269)
                     /gene="hemP"
                     /locus_tag="RH058_002423"
     CDS             complement(15078..15269)
                     /gene="hemP"
                     /locus_tag="RH058_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV3138001.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15401..16447)
                     /gene="aroH"
                     /locus_tag="RH058_002424"
     CDS             complement(15401..16447)
                     /gene="aroH"
                     /locus_tag="RH058_002424"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV3138002.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16604..17437)
                     /gene="ppsR"
                     /locus_tag="RH058_002425"
     CDS             complement(16604..17437)
                     /gene="ppsR"
                     /locus_tag="RH058_002425"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV3138003.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17770..20148
                     /gene="ppsA"
                     /locus_tag="RH058_002426"
     CDS             17770..20148
                     /gene="ppsA"
                     /locus_tag="RH058_002426"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV3138004.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20205..21851)
                     /gene="fadK"
                     /locus_tag="RH058_002427"
     CDS             complement(20205..21851)
                     /gene="fadK"
                     /locus_tag="RH058_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV3138005.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21908..22201)
                     /locus_tag="RH058_002428"
     CDS             complement(21908..22201)
                     /locus_tag="RH058_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV3138006.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22198..23487)
                     /locus_tag="RH058_002429"
     CDS             complement(22198..23487)
                     /locus_tag="RH058_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3138007.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHTQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23543..24481)
                     /locus_tag="RH058_002430"
                     /pseudo
     CDS             complement(23543..24481)
                     /locus_tag="RH058_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(24501..25265)
                     /locus_tag="RH058_002431"
     CDS             complement(24501..25265)
                     /locus_tag="RH058_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV3138008.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            25581..>26105
                     /locus_tag="RH058_002432"
     CDS             25581..>26105
                     /locus_tag="RH058_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV3138009.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGY"
BASE COUNT         6502 a         6223 c         6795 g         6585 t
ORIGIN      
        1 atgaacagct gacatgtttt catgattttt agcggccaac agtgtgaata attgacgttg
       61 actaaaagaa tatttttttg caaaagtact cagtgttgat atataagata acacgacgtc
      121 tttattaccc ttgctcatgg cgatgtataa agcaggggtt ccatgtgctg tcgcgccttt
      181 taataagtcc atgatgttga ttttgctgag tttatattta acggcgatcc cataaacttt
      241 agagaggaac cgtgtgaaac acaaagggtg attattttcc attgcagcaa ataaccctgg
      301 ttcaccatca cgtgttttca tggctaaaat atccatcacc atttttttgt ttaaaagttc
      361 ttgtgctatt ggagtggtta atgcattcag aaagatatcg attatttcat cgaatccata
      421 caacatggag atatagagtc caggtgtttc gttagcactt ttagtttgaa gtaatttaac
      481 cagattatct tcatgaatga tatgattgta taccagtgac tggatttctt gcataaatat
      541 ttttacgttg tcagtttgtc cgttcatcat agccagaaat aaagcaggtg taccaacgct
      601 attctgcgca gttattattt caatgatttc tttttcaggg agtatgggca actgtttgaa
      661 tagtgtaata agctattgag tctggcctgt ggccattatt aacaacattg ccgatggatg
      721 tataggggca aatggcattc gtgtcgtgat acccgctaaa atagcaactg gaactccctc
      781 aggtaataga ggaagaatag caattgcaca gttgcgaaca tacctatcag cccaggtctg
      841 agcatcggga cctgaactga tgaacttatc aagcgagaga aagccccgcg ctgtcctgtg
      901 ggtgcctctg acggcaacgt tggtatcgtt cgggtcgtaa acagacacaa cataatttaa
      961 ttgtccatac actgactgct ttattcgtag ccttactgac aaagtatgat tgtctatata
     1021 taaaatcgcg gacatatgac tgacattgtt tgccatcatt cgacaaaaga aaatatctaa
     1081 agcgaagcca aaatccttac tcgcgataac gcgactccct gcaggaggat ggcgaagtaa
     1141 tgctctgaac tccgcatccc ggactaaggg gatatgctgc tgtatggatt caacggagga
     1201 gtaatgttta atcaggttga ctttgccttt tccatttctc aatgaatcaa gggcgtattg
     1261 caatgacaga tggcgacaaa aaatagcgtc agaaggagat tgcaaaaaac atgcactacc
     1321 attgagattt actgttgggt gtatagtttt ttctctatgg atttttttag acagtttcat
     1381 acaatggtta cgatcttcag cagttaactg ttcctgttca atataatcca gtatgcttcg
     1441 caaatggtta gctttaatag gcgtggtttc cagtaataag gaaatgaaat cttttttata
     1501 gtgtaatgta atgagtatat tttctgcatg gatgggtgaa gtggcaaagt taatggtaaa
     1561 aagggcagtg gaataacaat tttgtgatat ttttcgacag tttgcggtaa attcctgaat
     1621 aacacagtat gacagcatcg aacttaagtc agaggtatca agtagggtga tgagctggcg
     1681 cgcaaattta ttaataggct ctgatgaagg tgttggtatg tcgctatcga tgtgcaatct
     1741 atgaggagac ttataatgag ttctgataat gatatcgttt tgactcataa tctattcgcc
     1801 ttatatatat ctggaggttt tgaggatgat gattaattat tatcgccaga acgaaatgtg
     1861 cacgaaaaat tgttataaga attgtgtcac ttttatcatt gtgatatgac cacttcgtca
     1921 ttaatctcat gagcgtttaa aatactaccg ccaaatcacg caacgaactt tgctggattc
     1981 agtttatgct gctgtaaatc cgctcgcagt aaacctttca gacgcaccgt gatgttatca
     2041 gtggttcttt aagcgttttg ctggtgtact cactacaaac gaattgcgaa tcgaatcaat
     2101 gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa catcgctcaa ccgggtggaa
     2161 ggtgagaggt taatgtcttt catttgtgta cctgattgcg aaccaattta gcatttgttg
     2221 gctagatggt ttcgcaatga actgttaata aacaaatttt tctttgtatg tgatctttcg
     2281 tgtgggtcac cactgcaaat aaggatataa aatgcctgtt ataactcttc ctgatggcag
     2341 ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca ttggtccagg
     2401 tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg cttgcgatct
     2461 gattgaaaac gacgcacaac tgtccatcat taccgccaaa gacgaagacg gtctggagat
     2521 cattcgtcac tcctgtgcgc acctgttagg gcacgcgatt aaacaacttt ggccgcatac
     2581 caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg atcttgaccg
     2641 cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa
     2701 aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa ctttcgccaa
     2761 ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg atgacaagcc
     2821 aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg taccgaacat
     2881 gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc gtggcgacag
     2941 caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa aagcgcttaa
     3001 cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca
     3061 gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc acaacgacgg
     3121 ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag agtaccagta
     3181 tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg
     3241 ggacaactac aaagatgcaa tgttcaccac gtcttctgag aaccgtgaat actgcattaa
     3301 gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt cttatcgcga
     3361 tctgccgctg cgtatggccg agtttggtag ctgccaccgt aatgagccgt caggttcgct
     3421 gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct tctgtactga
     3481 agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata tgtacagcac
     3541 ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac gtattggcag
     3601 cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag aaaacaacat
     3661 cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg aatttaccct
     3721 gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact tctccttgcc
     3781 gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac cggtaatgat
     3841 tcaccgcgca attcttgggt cgatggaacg tttcatcggt atcctgaccg aagaattcgc
     3901 tggtttcttc ccgacctggc ttgcgccggt tcaggttgtt atcatgaata ttaccgattc
     3961 acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca ttcgtgttaa
     4021 agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt tgcgtcgcgt
     4081 cccatatatg ctggtctgtg gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac
     4141 ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca
     4201 agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg
     4261 agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt
     4321 aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc tggagaaagc
     4381 agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc cggtttgtcg
     4441 tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg aacagaagaa
     4501 aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag atgaaggcga
     4561 ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata aagccaaaat
     4621 cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa
     4681 tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa cgaagatcga
     4741 aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc
     4801 actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg tttattaaca
     4861 atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg
     4921 cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc gtcacattct
     4981 gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg
     5041 cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg tttttaactt
     5101 tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt ggtgttattg
     5161 cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt gcgcgttctc
     5221 gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct taccgtgacc
     5281 gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac gcagcagcac
     5341 gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc tctgttgaaa
     5401 tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc accgctctgg
     5461 ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta gtctccctct
     5521 tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc aataaaggtc
     5581 ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc gcttcttttt
     5641 ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga aaacagcgcc
     5701 tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa aaagcctgtc
     5761 tcatcaggtg taattgtcgc agccagtctg gacacggcca gcgcggagaa accggagcgt
     5821 atttaagtac gtgagaattt cgagcactgc ccgggaccaa aatggcaagt aaaatagcct
     5881 gatgggatag gctctaagtc caacgaacca gtgtcaccac tgacacaatg aggaaaacca
     5941 tgtcacatct cgcagaactg gttgccagtg cgaaggcggc cattagccag gcgtcagatg
     6001 ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa aaaagggcac ttaacccttc
     6061 agatgacgac cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt gcggttatca
     6121 acgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa ctggaaagcg
     6181 ctgcactgaa tgcgcgtctg gcggcggaaa cgattgatgt ctccctgcca ggtcgtcgca
     6241 ttgaaaacgg cggtctgcat ccggttaccc gtaccatcga ccgtatcgaa agtttcttcg
     6301 gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga agacgattat cataacttcg
     6361 atgctctgaa cattcctggt caccacccgg cgcgcgctga ccacgacact ttctggtttg
     6421 acactacccg cctgctgcgt acccagacct ctggcgtaca gatccgcacc atgaaagccc
     6481 agcagccacc gattcgtatc atcgcgcctg gccgcgttta tcgtaacgac tacgaccaga
     6541 ctcacacgcc gatgttccat cagatggaag gtctgattgt tgataccaac atcagcttta
     6601 ccaacctgaa aggcactctg cacgatttcc tgcgtaactt ctttgaggaa gatttgcaga
     6661 ttcgcttccg tccttcctac ttcccgttca ccgagccttc tgcagaagtt gacgtcatgg
     6721 gtaaaaacgg taaatggctg gaagtgctgg gttgcgggat ggtgcatccg aacgtgctgc
     6781 gtaacgttgg catcgacccg gaagtttact ctggtttcgc cttcgggatg gggatggagc
     6841 gtctgactat gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa aacgatctgc
     6901 gtttcctcaa acagtttaaa taaggcagga atagattatg aaattcagtg aactgtggtt
     6961 acgcgaatgg gtgaacccgg cgattgatag cgatgcgctg gcgaatcaaa tcactatggc
     7021 gggcctggaa gttgacggtg tagaaccggt tgccggtagc ttccacggcg tggtcgttgg
     7081 tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga caaaagtgaa
     7141 tgttggcggc gatcgcctgc tggacatcgt ctgcggtgcg ccaaactgcc gtcagggcct
     7201 gcgtgtggcg gtagctacca ttggtgctgt tctgccgggt gatttcaaaa ttaaagcggc
     7261 gaaactgcgt ggtgaaccgt ctgaagggat gctgtgctcc ttttctgagc tgggaatttc
     7321 tgacgatcat agcggcatta tcgagctgcc tgcggatgcg ccgattggca ccgacatccg
     7381 tgaatacctg aaacttgatg acaacaccat cgaaatcagc gtgacgccaa accgtgccga
     7441 ctgtttaggt atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc tgccgctggt
     7501 tgaaccggaa atcgttccgg ttggtgcgac catcgacgac acgctgccga ttacagtcga
     7561 agcgccggaa gcctgcccgc gttatcttgg ccgtgtggta aaaggcatta acgttaaagc
     7621 gccaactccg ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt ctatcgatgc
     7681 agttgttgac gtcaccaact atgtactgct cgaactgggc cagccgatgc acgctttcga
     7741 taaagatcgc attgaaggcg gcattgtggt gcggatggcg aaagagggcg aaacgttggt
     7801 actgcttgat ggcactgaag cgaagctgaa tgctgacact ctggtcatcg ccgaccacaa
     7861 caaggcgctg gcgatgggcg gcatctttgg tggcgaacac tctggcgtga atgacgaaac
     7921 gcaaaacgtc ttgctggaat gcgctttctt cagcccgctg tctatcaccg gtcgtgctcg
     7981 tcgtcatggc ctgcatactg atgcgtctca ccgttatgag cgtggcgttg atccggcact
     8041 gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc gacatctgcg gtggtgaggc
     8101 tggtccggta attgatatca ccaacgaagc aacgctgccg aagcgtgcaa ccatcacttt
     8161 acgtcgtagc aaactggatc gcctgatcgg ccatcatatt gcggatgagc aggtaactga
     8221 cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa gacgagtggc aggcagttgc
     8281 gccgagctgg cgtttcgata tggagattga agaagatctg gtcgaagaag tcgcgcgtgt
     8341 ttacggctac aacaacatcc cggatgagcc ggtacaggca agcctgatta tgggtactca
     8401 ccgtgaagct gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgaca aaggctatca
     8461 ggaagtgatc acctacagct tcgttgatcc gaaagtgcag cagatgatcc atccaggcgt
     8521 tgaagcctta ctgctgccaa gcccgatctc tgttgaaatg tcagcaatgc gtctttctct
     8581 gtggaccggc ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga accgtgtgcg
     8641 cattttcgaa agcggtctgc gtttcgtacc agatactcag gcaccgttgg gcattcgtca
     8701 ggatctgatg ttagccggtg tgatttgcgg taaccgttac gaagagcact ggaacctggc
     8761 aaaagagacc gttgatttct atgatttgaa aggcgatctt gaatccgttc tcgacctgac
     8821 cggtaaactg aatgaggttg agttccgtgc agaagcgaat ccggcactgc atccggggca
     8881 atccgcagcg atttatctga aaggtgaacg tattggtttt gttggggttg ttcatcctga
     8941 actggaacgt aaactggatc ttaacggtcg cactctggtg ttcgaactgg agtggaacaa
     9001 gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg cgaaccgtcg
     9061 tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg gatattttat ccgaatgtaa
     9121 gaaagttggc gtaaatcagg tagttggcgt aaacttattt gacgtgtacc gcggtaaggg
     9181 tgttgcggag gggtataaga gcctcgccat aagcctgatc ctgcaagata ccagccgtac
     9241 actcgaagaa gaggagattg ccgctaccgt cgccaaatgt gtagaggcat taaaagagcg
     9301 attccaggca tcattgaggg attgaaccta tggcgcttac aaaagctgaa atgtcagaat
     9361 atctgtttga taagcttggg cttagcaagc gggatgccaa agaactggtt gaactgtttt
     9421 tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct ggttttggta
     9481 acttcgatct gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg ggcgaggata
     9541 ttcccattac agcacggcgc gtggtgacct tcagacccgg gcagaagtta aaaagccggg
     9601 tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc gctctgcggc
     9661 cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca cttcagcaga
     9721 acggatacca tgctgacact tgcccgccaa caacagcgac aaaatattcg ctggttatta
     9781 tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg tgaacaatgg
     9841 atctcgccag gtgactggtt tactcctcgt ggcgaactgt tcgtctggca aattcgcctg
     9901 ccacgtacgc tggctgtatt gctggttggt gcggcgctgg ctatatccgg cgctgtaatg
     9961 caggcgttgt ttgaaaatcc tctggcagaa cctggactac ttggcgtctc taacggcgca
    10021 ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc aactccccaa ctgggcgcta
    10081 gggctgtgtg cgattgctgg cgcgcttatc atcactttaa tactcttacg tttcgcccgt
    10141 cgtcatcttt cgaccagtcg gttattgctg gctggcgttg cattagggat tatctgtagc
    10201 gcactaatga cgtgggctat ctacttttcc acctctgttg atttacgtca gctgatgtac
    10261 tggatgatgg gcggttttgg cggcgtagac tggcggcaaa gctggctgat gctggcattg
    10321 atccccgtgt tgttgtggat ctgttgtcag tccaggccga tgaatatgtt agcacttggc
    10381 gagatctcgg cgcggcaact gggtttaccc ctgtggttct ggcgcaatgt gctggtggcc
    10441 gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg gtgctatcgg ctttattggt
    10501 ctggtgatcc cacatattct tcggttgtgt ggtttaaccg atcatcgcgt attacttccc
    10561 ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg atattgtagc gcgcctggca
    10621 ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc gccggtgttt
    10681 atctggttat tgttaaaagc aagacgttag ccgcaaaaag acggtctatg attaaaagct
    10741 aagattttta ctgaccacac ccaggagaaa cgatgcaaga ttccattctg acgaccgtag
    10801 tgaaagatat cgacggtgaa gtgaccacgc tggagaagta cgctggtaat gtgctgttga
    10861 ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg gagaatattc
    10921 agaaagcctg ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac cagtttctgg
    10981 aacaagaacc gggcagcgat gaagagatta aaacttactg taccaccaca tggggggtga
    11041 cgttcccgat gttcagtaag attgaagtta atggcgaagg acgccatccg ctgtatcaaa
    11101 aattgattgc cgcagcgccg accgcagtcg cgccggaaga gagcggattc tatgcccgta
    11161 tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat tttatggaat tttgagaaat
    11221 ttttagttgg cagggacgga ttggtcatcc agcgtttttc cccggatatg acgccggaag
    11281 accccattgt gatggaaagc attaaactgg cgttggcaaa ataatgtcta ttgtgatgca
    11341 gttacaggat gttgcggaat ctacccgcct ggggccactt tctggcgagg ttcgggctgg
    11401 ggagatcctg cacctggtgg ggccgaatgg cgcgggtaag agtaccttgc ttgcacgaat
    11461 ggccggaatg accagcggta agggaagcat tcagttcgcg gggcaacccc tggaagcatg
    11521 gtccgcaaca aaactcgcgc tgcatcgcgc ctatctttca caacagcaga cgccgccgtt
    11581 tgcaatgccg gtctggcact acctgacact gcatcagcac gataaaacgc gtaccgaact
    11641 actgaatgat gtcgcagggg cgctggctct tgatgacaaa ctcggacgta gcaccaatca
    11701 actttccggc ggtgaatggc aacgcgtacg tcttgctgcg gtggtgctgc aaatcacacc
    11761 acaagccaat cccgcaggtc aattgctgct tcttgatgag ccgatgaaca gtcttgatgt
    11821 tgcgcaacaa agtgcgttag acaaaattct gagcgctctg tgtcagcaag gactggcgat
    11881 tgtgatgagc agtcacgatc tcaaccacac attgcgtcat gcgcatcggg cgtggttgct
    11941 aaaaggtgga aaaatgctgg ccagtggacg cagagaagag gtgctcacgc cgccaaatct
    12001 ggcgcaggcc tatgggatga attttcgccg tctggatatc gaaggtcaca gaatgctgat
    12061 ttcgaccatc tgatttcatt agtatcttga aaatcgggta attacgacgc taaattaatg
    12121 caagaataaa aaacagagga accgtacgga atgcgtttct gccttatttt gatcacagca
    12181 ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc caaatgccag actttctgat
    12241 tcgattaccg ttattgccgg tttgaacgac cagctacaaa gctggcatgg cacgccgtat
    12301 cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt tgtaacgatg
    12361 cgcgatcgtt tcgatttgca gctgccccga gaaaccaaag aacaagcctc tatcggcacg
    12421 caaattgata aagacgagtt gctgcctggt gacctggtct ttttcaaaac ggggtccgga
    12481 caaaatggtt tgcatgtagg tatttacgat accaacaacc aatttatcca cgcctctacc
    12541 agcaagggag tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa tttctggcag
    12601 gcgagacgaa tctagtatgt cgaaaaatgg acggcgatga tacatcgccg tccatttact
    12661 cctgaataca gtagtatttt gcagacaaaa attccgtaaa tggattattc gtttataatc
    12721 aggataatct aaaaaaagga cccctgaaaa agtggttaac atggaacaaa tgaaagttac
    12781 tccttctgta attgttatgt cttatggcct catccctttt agccggatac tgaaaaacat
    12841 ccttcgagag ggacggttac catgaagatt tttttggaga atctttatca ttctgattgc
    12901 tatttcctgc cgataagaga taaccagcaa ctccttgtcg gcgttgagtt aattacccat
    12961 ttctccagtg aagatggcac ggtgcgtatc ccgacgagtc gggttatagc gcaacttacc
    13021 gaggagcaac attggcaatt attttcagag cagttggaat tactaaaatc atgccaacat
    13081 ttttttattc aacataaact ctttgcctgg cttaatttaa cgccacaagt ggctacatta
    13141 ttattagaga gggataatta cgcaggcgaa ctattaaaat atccgtttat tgaactgttg
    13201 attaatgaga attacccgca tctcaatgaa ggaaaagata atcgcgatct tttatcctta
    13261 tcgcaaatgt atccccttgt gttaggcaat ttgggagcag gtaatagcac aatgaaagcg
    13321 gtttttgatg gtcttttcac ccgcgtcatg ctggataaaa gttttattca gcaacaaatc
    13381 acgcatcgct ccttcgaacc gtttattcgt gcgatccagg cgcaaatttc cccttgttgc
    13441 aattgcatca tcgcgggggg cattgatacg acagaaatat tggcacagat tacccccttc
    13501 gatttccatg ctctccaggg atgcctgtgg cctgccgttc ctataaatca gataacgacg
    13561 ttggttcagc gataaccctc ctgtttgctg gtgtttaaga cgagagtaac cgtctacact
    13621 atcaaacagg aggatctatg accctgtctt ttattacccg ctggcgcgat gaattgccag
    13681 aaacctatac agcactttcc cctacgcctt taaataatgc ccggctgatt tggcataata
    13741 ccgaactcgc taacacgctg agtattccat cgtcgctgtt taaaaatgct gcaggtgtct
    13801 ggggcggcga aaccctactg cctggtatgt caccactggc gcaggtttac agtggtcatc
    13861 agttcggcgt ctgggcgggc caactgggtg atgggcgcgg cattttactt ggcgaacaac
    13921 tgcttgctga tggcactaca atggactggc atctgaaagg tgctggcctg acgccttatt
    13981 cgcgaatggg tgatggacgg gcggttttac gttcgacgat acgagaaagt ctcgccagtg
    14041 aggcgatgca ttatctgggc attccgacga cccgcgcgtt aagtatcgtc accagcgatt
    14101 cgccagtgta tcgggaaacg gtggagccag gcgcgatgct gatacgtgtg gcaccaagtc
    14161 atctgcgctt tggtcatttc gaacattttt actatcgccg cgagccggaa aaggttcgtc
    14221 agttagctga ctttgccatt cgtcattact ggtcatatct tgaagatgat gaggacaaat
    14281 accgcctctg gtttagcgat gttgtcgcac gtaccgcatc gttaattgcc caatggcaga
    14341 cggtcggctt tgctcatggg gtgatgaata ccgataacat gtcgctgctg gggctgacgc
    14401 ttgattacgg gccgtttggt tttctggatg attacgaacc cggttttatt tgtaatcact
    14461 cggatcatca agggcgttat agctttgata atcaacctgc tgtcgcgttg tggaatttac
    14521 atcgtctggc gcagacattg tcaccatttg ttgccgtaga tgccctgaat gaggccctgg
    14581 acagctatca gcaggttttg ttgacgcatt atggacaacg gatgcggcag aaactgggct
    14641 tcatgacgga gcaaaaagag gataacgcgc tactgaatga attattcagt ctgatggcgc
    14701 gagagcgcag cgattatacc cgcacattcc gcatgctgag tctgaccgag cagcacagcg
    14761 cggcgtcacc gctacgtgat gagtttattg atcgtgcggc atttgatgac tggtttgccc
    14821 gttatcgggg gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag cagctgatgc
    14881 aaagcgttaa cccagctctg gtgttgcgca actggttggc gcaacgggcg attgaagcgg
    14941 cagaaaaggg tgatatgacg gaattgcacc gcctgcatga ggcgttgcga aatcctttca
    15001 gcgacagagc tgatgactat gtcagtcgtc cacctgactg gggtaaacgg ctggaagtca
    15061 gctgctcgag ttaaaggcta cttggtcaac agcagcttgc cagcctgagt tttacggagc
    15121 agatattctt gcccgtcatg atcgataatc agtttgccat ccggacctaa cagtgtctgg
    15181 ctggaaattc gccggatggg ctgcggggaa gcggtcttgt gattggcatc cgtttcaggc
    15241 gtgagttttc tgctatccgt ataacgcata aaaattaata ataattgata taacaatgat
    15301 tcttattatc aatattatgg ctgaccagag caagcagtaa tgtgctgaga agacaacaag
    15361 ttgcgcagaa atgcccatcc catcaggaat gggcacgcat tcagaagcgg gtatctaccg
    15421 cagaggcaag tttttcgacc aggcgttcgg tatcttccca gcccagacac gggtcggtaa
    15481 tggattgacc gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct tcgcgcagga
    15541 aactttccgc cataattcca gcaatcgccg tagagccatt gcggatttgc tgacaaatat
    15601 cctcacaaac ttctaactgg cgacggtgct gcttctggca gttaccgtgg ctgaaatcca
    15661 ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg gcgatatcat
    15721 cggcatgata attcggtttt ttgccgccac gcataataat gtggccatac gggttgccgc
    15781 tggtctgata gatggtcatc tgaccatttt tgtctggcga gagaaacata tggctggcgc
    15841 gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc atcggtacca tttttaaaac
    15901 ctaccggaca ggagagtgcc gaagccattt cacggtggat ctgactttcg gtagtacgtg
    15961 cgccaatcgc gccccaactg attaaatcag caataaactg accggtcacc atatcgagga
    16021 actcagtcgc ggtagggaca cccagctcat ttacctgtaa aagtaatttg cgcgccagct
    16081 ccagaccgtg atttacccga tagctgccgt ttaaatccgg atcggagatt agccctttcc
    16141 agccgacaac agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc agccgtgact
    16201 ggtactggtt gcgcagcgac tgcagacggg tggcgtactc cattgcagcg gtgagatcgt
    16261 ggatcgagca ggggccaata atgaccaaca gtcgcttatc ttcaccattc agtatttttt
    16321 caattctgcg gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg ggataccgta
    16381 gcgcgagttc ggcgggcgtt accaggctct caatacgcgc agtacggagt tcgtcagttc
    16441 tgttcattac aagtctcaga aatttgttgc ttctacggca ggactaccgg gaagtgatgc
    16501 gcatcacgat aaaccaatcg cagctgattt caagtgcgag gtgtgtcaca cctcgccggg
    16561 tggttagcat gataacaaaa aaataagcta atgcactagt tctctagtac attcggcggc
    16621 taagccccat gatatcgagg atcttggtgg caatctcttc taccgaataa ttggtactgt
    16681 taatccacgg gatctgattt ttacggtaca acgcttctac ttccgcgact tccatcctgc
    16741 actgacgaag cgaggcatag cggctgttct cccgacgttc ctcgcgaatc gccgccagac
    16801 gttccgggtc gatagtcagg ccgaacaatt tatgctgaag cggtttgagc gacgcgggta
    16861 gcaccagatt gtccatatcg tcggcaataa aggggtagtt tgccgcgcgg ataccaaact
    16921 gcattgccag atacagactg gtgggggttt taccacagcg agaaacaccg agcaggatca
    16981 cctgagcctg gtccagattg cgcaacgaaa tgccgtcatc gtgggcgagg gtgtaatcaa
    17041 tcgccgcaat gcgcgcatca tatttattga gattattagg gttaaggcca tgggtacgat
    17101 gagcaatcgg cgttggatcc agtttcatct cttgttgtag cggggcaacc agcgcctgaa
    17161 caatatcctg gcaaaagcct tcactttgca agatgatggc gcgaatctcc ggcaacacga
    17221 tggagtagaa gaccagcggg cgcacacctg tctggtgata aattgcgtca atctgatcct
    17281 tcactgcccg tgcacggctc tcattttcga caaacggcag cgtaatgctg ctgatagtga
    17341 cgggaaattg tgacattact gcgtgtccta atacctccgc agttattgcc gtaccatcag
    17401 aaatataaaa aacgtggcga tcaacagcat tatccatttt gttcttcccg tgatgcagac
    17461 ataattctat gaaagcataa attaaaaacg cacagaagcg taggacgtaa tgtctggttt
    17521 ataaaatgaa ccttcaattt tattttttat gaaaacagca tttcattttt atggtttcgt
    17581 ttataccgat ggtttatgtg gaaattgtcg aatagagcag gtttgcgcaa cgctgggatc
    17641 agtcttaaaa agtaaaaaaa tatatttgct tgaacgattc accgtttttt tcatccggtt
    17701 aaatatgcaa agataaatgc gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag
    17761 gattgttcga tgtccaacaa tggctcgtca ccgctggtgc tttggtataa ccaactcggc
    17821 atgaatgatg tagacagggt tgggggcaaa aatgcctccc tgggtgaaat gattactaac
    17881 ctttccggaa tgggtgtttc cgttccgaat ggtttcgcca caaccgccga cgcgtttaac
    17941 cagtttctgg accaaagcgg cgtaaaccag cgcatttatg aactgctgga taaaacggat
    18001 attgacgatg ttacccagct tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac
    18061 actcccttcc agcctgagct ggaaaatgcc atccgcgaag cttatgcaca gctttccgcc
    18121 gatgacgaaa acgcctcttt tgcggtgcgc tcctccgcta ctgcagaaga tatgccggac
    18181 gcttcctttg ccggtcagca ggaaaccttc ctcaacgttc agggttttga cgccgttctc
    18241 gtggcagtga aacatgtatt tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg
    18301 caccagggtt acgatcaccg tggtgtggcg ctctccgccg gtgttcaacg gatggtgcgc
    18361 tctgacctcg catcttctgg cgtgatgttc tccattgata ccgaatccgg ctttgaccag
    18421 gtggtgttta tcacttccgc atggggcctt ggtgaaatgg tcgtgcaggg cgcggttaac
    18481 ccggatgagt tttacgtcca taaaccgaca cttgcagcga atcgcccggc tatcgtgcgt
    18541 cgcaccatgg ggtcgaaaaa aatccgcatg gtttacgcgc cgacccagga gcacggcaag
    18601 caggttaaaa tcgaagacgt accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa
    18661 gaagtgcagg aactggcaaa acaggccgta caaattgaga aacactacgg tcgcccgatg
    18721 gatattgagt gggcgaaaga tggccacacc ggcaaactgt tcattgtgca ggcgcgtccg
    18781 gaaaccgtgc gctcacgcgg tcaggtcatg gagcgttata cgctgcattc acagggtaag
    18841 attatcgccg aaggccgtgc tatcggtcat cgcatcggcg cgggtccggt gaaagtcatc
    18901 catgacatca gcgaaatgaa ccgcatcgaa cctggcgacg tgctggtcac tgacatgacc
    18961 gacccggact gggaaccgat catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt
    19021 cgtacctgtc acgcggcgat catcgctcgt gaactgggca ttccggcggt agtgggctgt
    19081 ggtgatgcaa cagaacggat gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt
    19141 gataccggtt acgtctatgc ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa
    19201 acgatgccgg atctgccgtt gaaagtgatg atgaacgtcg gtaacccgga ccgtgctttc
    19261 gacttcgcct gcctgccgaa cgaaggcgtg ggccttgcgc gtctggaatt tatcatcaac
    19321 cgtatgattg gcgtccaccc acgcgcactg cttgagtttg acgatcagga accgcagttg
    19381 caaaacgaaa tccgcgagat gatgaaaggt tttgattctc cgcgtgaatt ttacgttggt
    19441 cgtctgactg aagggatcgc gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc
    19501 cgtctctctg attttaaatc gaacgaatat gccaacctgg tcggtggtga gcgttacgag
    19561 ccagatgaag agaacccgat gctcggcttc cgtggcgcgg gccgctatgt ttccgacagc
    19621 ttccgcgact gtttcgcgct ggagtgtgaa gcagtgaaac gtgtgcgcaa cgacatgggg
    19681 ctgaccaacg ttgagatcat gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg
    19741 gttgaagaac tggcgcgtca ggggctgaaa cgtggcgaga acgggctgaa aatcatcatg
    19801 atgtgtgaaa tcccgtccaa cgccttgctg gcagagcagt tcctcgaata tttcgacggc
    19861 ttctcaattg gctcaaacga tatgacgcag ctggcgctcg gtctggaccg cgactccggc
    19921 gtggtgtctg aactgttcga tgagcgcaac gatgcggtga aagcactgct gtcgatggcg
    19981 attcgtgccg cgaagaaaca gggcaaatat gtcgggattt gcggtcaggg tccgtccgac
    20041 cacgaagatt ttgccgcatg gttgatggaa gaggggatcg atagcctgtc tctgaatccg
    20101 gacaccgtgg tgcaaacctg gttaagcctg gctgaactga agaaataaaa taaatacccg
    20161 gcggcgtttt gtcgccgggt ttatgtgatc cccgaagatg aaacttattc aatctcttca
    20221 cagacatcct gcgttaatcg ccgcataata tcttttctta acaaaaactt ttgtatttta
    20281 cctgaggcag tgcgcggtag tttttcgatt accacgatat gttcaggata tttatatttt
    20341 gcgacccgtt tacggctaaa aaaagccacc acctcttcca gcgataatga atgatgcggc
    20401 gctttcagca cgacataagc gcatgatcgt tcacctaaac gttcatcggg cattgcaacc
    20461 acacaggcat cgtgaatttt aggatgctgc aataaaatat cttccacttc acggctgcta
    20521 atattttcgc cgccgcggac aataatatct tttttgcgtc cggtaatttt tatatagcca
    20581 gcctcatcca tacggcagag atcgccgctg taataccagc cttcttcgtc cagcgcacgg
    20641 gcggttaatt caggttcatc aaaatacccc ataaacacat tggggccacg cgaggcttct
    20701 tcaccttcgc aacctggcgg taaggtcttg cgtgcgtcat cgaccacttt aatctctaca
    20761 cctgcggcag cgtaaccatc ggtgtgcata aagcgcgaca aaggatcatc gagattcacc
    20821 accgcatgcg gcgaactttc tgtggaacca taaacactta ataatttaat gccgcgctgc
    20881 tggcattcac gtgccacttt tttggggatt gtggtaccgc cgcaaagaaa gaaacgcagc
    20941 gctgaaaggt ccgcgggttg tttctctagt acattcaaaa gatcatagac aaacggcgtt
    21001 gcgcctagca tacaggtgca acgctgctgc tcaagcagcg cgagacacgc atcaggagtg
    21061 aaaatatcta acaacacgct gcgagcgcca attaagaatg gtgccgttac gccatgcaga
    21121 aagcccgttg cgtgaccaag tggcgcaggc atcataaaga catcctgcca ggtcagattc
    21181 agtcgcgcgc aataagcccg ctcgctggcg agaatattgt tatgcgttag catcacgccc
    21241 tttggcagac cctcggttcc ggaggtaaac agcaccgcag ctaattcatc gccgtgggtc
    21301 gttatcgccg tggtcagtga ggtattgtcg gcgataatct gacttaatga gagggaagag
    21361 gtggcgggag ccagtttgtc cacaccgaca atttgttgta gttgtggaag ctgattttgc
    21421 agcggcagga ttaaatctac cggacgcgtt tgtttaaaca acgtcggtgc aaagaacatt
    21481 tttgcctgac acttattgag cacccacacc agttctgctt cccgccagga aggcaacagc
    21541 ggcacggaaa ccgcaccggt tttcaggcag gcaagataga taacggtaaa ttcacaccag
    21601 cctggcagtt gaaatgcgat gcgatcgcct gattcaatac ccttcgctaa catccagttt
    21661 gccagacagc tcgcggcgtg atcgagcgcg ctataggtgt acgatgctcc atgattatcg
    21721 accacggcaa ttttgtctgg catcgcacga gcggtctgct gccagtaatc ggccagcgaa
    21781 gcatcgcccc atagcccttg ctgacgatac gccgcacgac gttgttcgtt gaacgttaat
    21841 gtcactttca tgttggactc tcgaaacaga acatcaggcc cgagatgcgg gcctgtggga
    21901 tgcatgatta gccgtagcga aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt
    21961 cgagaatagt gttaccgcac agcacccgac aggtgccgca ctccagacaa ccggcagaat
    22021 caaaatgaat gttgccggcg tcatcctgct tataaagtcc ggcagggcag gctttcatta
    22081 atttacggaa ttcattgata tcgggatttg ccgccagaat gatatgcggg tggccctcat
    22141 caacatggaa tttattgacg cctaatttga tgtcgacgtt aaccgtagcg ttctggctca
    22201 tagcgcggtt gctcccttaa tgccatcttt cagcaagttg atcagcccaa ttttcttcgc
    22261 gtgtcccatg atcattttgc gtaccggctg gtttggtttg ccgtcaatgg tgaacatctc
    22321 gttcatgatg tcggcgacca ttcgtgggta ttggctaaac aggcgcgggt tttccatcag
    22381 cgccgggatc ttgcggaaat gctgcatatc gcgcatgacg cagctttgtt ccagctcacg
    22441 tttgtattgc gccagactgc tggcggagaa atcctcgcgt tctttggcgg cgatcaccgt
    22501 tgtggcggca gcctgagccg atgcaatggc taaatccatg ccacgaactg taaaacccaa
    22561 attcaggcag aagcctgcgg cgtcaccaac gatcatcacg ccatcgttaa ccatctgcgg
    22621 caccattgcc agaccgcctt ctggcaccat atgcgcggaa tattcaagca gtttgccgcc
    22681 gctaattagt gggcgaatgg cggggtgttg tttaaaatct tccagcattt gcggcacgct
    22741 tttttgcgta tgggcgatat cacccaatcc acaaaccagc cccaaggata tggaatcctt
    22801 gttggtatag agaaatcccc cgcccatcag gccgtcagaa ggggaaccgg caaacagcca
    22861 ggcggcacct tcctcgcccg taatattaaa gcgatcgttg atctgttctg gtgtgaggcc
    22921 aataacctct ttaacaccaa cagcgtaatg atgcggatcg gaagcgggaa ccattcccag
    22981 cgagcggcca agcatcgagt taacgccatc agccagaatc accacattcg cttcgagaat
    23041 atcatcccca gcctgcacgc cagtgacctt gtttccttca cgaaccagcg catcgacgcg
    23101 aacgcctggg ataaactgcg cgcccgcctg ctcggcttgt tccatcaacc acgggtccag
    23161 acgattacgc aatacggtat aagatgcgtg ttgcggaaca tctggttgct cgcggtgaaa
    23221 atcgagggta acggcgcttt cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg
    23281 ttctaccggc gctgatactg caaagcctgg aatgattgct tcaagtgtgt gggcataaag
    23341 acgcccgccg gtcatgtttt tacatccggc actgtcgccg cgttctatca ccaggacatc
    23401 cagcccggct cgcgccatga ccagtgcggc aacgctacca gcaacgcccg caccgaccac
    23461 aatggcatca aatttgtctt ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg
    23521 ggagatgtgg ttgaaagggg aattagcggc tcaactggct aatcagggca gggacgactt
    23581 tgtagatatc gcccaccaga ccgtagtcgg cataattgaa gattggcgca tttttatctt
    23641 tattgatggc gacaatcact tttgcgccgt tgccgccaac catatgctgg atctgcccgg
    23701 ggatccccag cgtcaggtag agatcggatt tcagcaacac gccggagaca ccgatataac
    23761 gttcacgctc catccagttc tcgccttcgg caattggacg tgaacagccg acttcagcat
    23821 tcagcaccgc cgccagttcg tggaccattt tcaggtcatc ctgcgccgcc agaccacgac
    23881 cgacgccaac cacacgtttt gctttgctca ggtccacgct gcttgcggct ttcgcacggc
    23941 gttcgcgaca gagaatttca tgacgcggag caacataagg taccgtttct gtcgggcact
    24001 gatgagaggt atcactggtg cacggttcct gaacaccggg tgcaagggta atgatcgcca
    24061 gcgggctgtt gatcttttcc tgagcgaacg ctaacccgcc atacatccgg tgttcggcgc
    24121 aaatgtgacc atcgacaata tccaccgccg tggcatcgtt caccagcgcc gcattcagtt
    24181 gcacacttaa ccgtgctgcc agagctttac cacgtttcgt cgcggccaac agcaacatag
    24241 cggggtgttt atctttcagc agggcagcaa tgctttcggc gtaattttca gtgcgttgca
    24301 gcgcgtcgtt ttgctcaaga acataaatac attttggacc ataaggcata actgcctgcg
    24361 cctagtcggt attttgtaca atggcataca cctgttggcc ccattgctga gcgccgccaa
    24421 acagttcagc ataacgttca ggattatcgc taaagaccca gacgctgttt aattgactca
    24481 tcgtttcttc tccgtaggct tcagttcagg gctttcttca gatgttccgc cagctcggca
    24541 atggcctccg gcgaatcgtt atcgataatg atgtgcttac gttctgtttg cggcggtacg
    24601 cgaatgccga ccagttcagc aagtggcgcg ctctggctcc agtcaatatc acttgcctgc
    24661 cactgattta ccggtttttt acccgcgccg agaatggctt tcatcgaagg aatgcgtggc
    24721 acgttaatat cggaggtgac gcagagcacg gctggaacag agagttcaat aacttcaaca
    24781 tcatcttcaa gcgtgcgttc aatcaccagt gtattgccct gacgctgaat agcactcact
    24841 gcattaatca ccggaagttg cagaatttct ccgaccagca agccaacctg ctgggcataa
    24901 aggtcgccgg aaccttcacc aaagatcagt aaatcgaagc cgatcttttc aattgctgcc
    24961 gccagcgctt ttgcggtatc gagaggcagt gcatgttcaa gttgcgcatc ctgcaccaaa
    25021 tacaggctgt gcggcccgcg ggatagcacg tctttgcgca ctttcgagtt ctgcaacaat
    25081 gagccgccaa cggtcagcgc ggctatctca tcgtcatctg ttgcgagctg gcttgcagct
    25141 tcaatggcat tgagatcgaa ctggctgatt ttggcgtcgg cattgtcgaa attcagggtg
    25201 tattctggag tgacaacaat gtcctgttct tcaggcacca gcttaaagca ggttattatt
    25261 ttcatgattt ctcctgtgag tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc
    25321 tgaaaatagt gtgcgatagc ggtgcggggt tttcgacgga gcaagctgtt ttccttgcca
    25381 acaatcttgc aaaaaaaatg cgatcgctaa aacaaaagtg caatttgcgg caatatagtt
    25441 attccatgac cgtacaatct tgcattcagt gtaaataatc ttgtttagct ctcgctaaac
    25501 gcggttgtat aaccgcaata cacgccgtat tttatccggc atatattgtt tttgcttgct
    25561 catggaatag ggaggttgtc atgtatcaac gctgttttga taacgccagt gaaacgctgt
    25621 ttgtcgccgg taaaacgcca cggctttcac gttttgcatt tagcgatgat ccaaaatggg
    25681 agtccggaca tcacgttcat gacaatgaaa ccgagttgat ttacgtcaag aaaggggttg
    25741 caaggtttac catcgattct tcgttatatg tcgcacatgc ggatgacatt gtggtgatag
    25801 aacgcggcag gctacatgcg gtggcctctg acgttaacga tccggcaacg acgtgtacct
    25861 gtgcgctgta cggctttcag tttcaggggg ctgaggaaaa tcagctactg caaccgcatt
    25921 cttgtccggt aattgccgcg gggcagggaa aagaagtcat aaaaacctta tttaatgagc
    25981 taagtgtgat tttgccgcaa agtaaaaata gccaaacatc ttcgttatgg gatgcatttg
    26041 cctatacgtt agccattctt tactacgaaa attttaaaaa tgcttatcgt tcggagcagg
    26101 ggtat
//
LOCUS       DAPHDX010000060        25903 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPHDX010000060 DAPHDX010000000
VERSION     DAPHDX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.060"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1305
                     /gene="pgaA"
                     /locus_tag="RH058_002433"
     CDS             <1..1305
                     /gene="pgaA"
                     /locus_tag="RH058_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV3138010.1"
                     /translation="VTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDL
                     PQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQ
                     AWTALTLQEWQQAVVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGP
                     DSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWL
                     EAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQA
                     YVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNT
                     EHDTPYYNPIKTFDIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLG
                     YGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVEFDMTFRF"
     gene            1314..3332
                     /gene="pgaB"
                     /locus_tag="RH058_002434"
     CDS             1314..3332
                     /gene="pgaB"
                     /locus_tag="RH058_002434"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDV3138011.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            3325..4650
                     /gene="pgaC"
                     /locus_tag="RH058_002435"
     CDS             3325..4650
                     /gene="pgaC"
                     /locus_tag="RH058_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV3138012.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            4652..5065
                     /gene="pgaD"
                     /locus_tag="RH058_002436"
     CDS             4652..5065
                     /gene="pgaD"
                     /locus_tag="RH058_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDV3138013.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(5115..5903)
                     /gene="phoH"
                     /locus_tag="RH058_002437"
     CDS             complement(5115..5903)
                     /gene="phoH"
                     /locus_tag="RH058_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV3138014.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6522..7793)
                     /gene="efeB"
                     /locus_tag="RH058_002438"
     CDS             complement(6522..7793)
                     /gene="efeB"
                     /locus_tag="RH058_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV3138015.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGKHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(7799..8926)
                     /gene="efeO"
                     /locus_tag="RH058_002439"
     CDS             complement(7799..8926)
                     /gene="efeO"
                     /locus_tag="RH058_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV3138016.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(8984..9814)
                     /locus_tag="RH058_002440"
     CDS             complement(8984..9814)
                     /locus_tag="RH058_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV3138017.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGFFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(10356..11864)
                     /gene="putP"
                     /locus_tag="RH058_002441"
     CDS             complement(10356..11864)
                     /gene="putP"
                     /locus_tag="RH058_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV3138018.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGVSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARSISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(12023..12232)
                     /locus_tag="RH058_002442"
     CDS             complement(12023..12232)
                     /locus_tag="RH058_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138019.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            12287..16249
                     /gene="putA"
                     /locus_tag="RH058_002443"
     CDS             12287..16249
                     /gene="putA"
                     /locus_tag="RH058_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3138020.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(16289..16927)
                     /gene="rutR"
                     /locus_tag="RH058_002444"
     CDS             complement(16289..16927)
                     /gene="rutR"
                     /locus_tag="RH058_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV3138021.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            17215..18306
                     /gene="rutA"
                     /locus_tag="RH058_002445"
     CDS             17215..18306
                     /gene="rutA"
                     /locus_tag="RH058_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV3138022.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            18306..18998
                     /gene="rutB"
                     /locus_tag="RH058_002446"
     CDS             18306..18998
                     /gene="rutB"
                     /locus_tag="RH058_002446"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV3138023.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            19010..19396
                     /gene="rutC"
                     /locus_tag="RH058_002447"
     CDS             19010..19396
                     /gene="rutC"
                     /locus_tag="RH058_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV3138024.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            19404..20204
                     /gene="rutD"
                     /locus_tag="RH058_002448"
     CDS             19404..20204
                     /gene="rutD"
                     /locus_tag="RH058_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV3138025.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPANWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            20214..20804
                     /locus_tag="RH058_002449"
     CDS             20214..20804
                     /locus_tag="RH058_002449"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV3138026.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            20815..21309
                     /gene="rutF"
                     /locus_tag="RH058_002450"
     CDS             20815..21309
                     /gene="rutF"
                     /locus_tag="RH058_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV3138027.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            21330..22658
                     /gene="rutG"
                     /locus_tag="RH058_002451"
     CDS             21330..22658
                     /gene="rutG"
                     /locus_tag="RH058_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV3138028.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSKRLVDVPPPEVVHQEP"
     gene            complement(22741..22914)
                     /locus_tag="RH058_002452"
     CDS             complement(22741..22914)
                     /locus_tag="RH058_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV3138029.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            23287..23883
                     /gene="wrbA"
                     /locus_tag="RH058_002453"
     CDS             23287..23883
                     /gene="wrbA"
                     /locus_tag="RH058_002453"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV3138030.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            23904..24131
                     /locus_tag="RH058_002454"
     CDS             23904..24131
                     /locus_tag="RH058_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV3138031.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(24169..25410)
                     /gene="agp"
                     /locus_tag="RH058_002455"
     CDS             complement(24169..25410)
                     /gene="agp"
                     /locus_tag="RH058_002455"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV3138032.1"
                     /translation="MNKTLIAAAVAGIVLIASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(25699..>25903)
                     /locus_tag="RH058_002456"
     CDS             complement(25699..>25903)
                     /locus_tag="RH058_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138033.1"
                     /translation="IVNSNMASISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFA
                     QAYKLNNKEMNAQLDYLNRYAIY"
BASE COUNT         6274 a         6741 c         6592 g         6296 t
ORIGIN      
        1 gtcacccaac ataccattaa tacttcgccg cctttccttc ggttaatggg cacgcctacg
       61 agcatcccga atgatacctg gttacagggg cattcgtttc tctcaaccgt agcaaaatat
      121 agtaatgatc ttcctcaggc tgaaatgaca gccagagagc ttgcttataa cgcaccagga
      181 aatcagggac tgcgcattga ttacgcgagt gtgttacaag cccgcggttg gcctcgtgca
      241 gcagaaaatg aattaaaaaa agcagaagtg atcgagccac gtaatattaa tctggaggtt
      301 gaacaagcct ggacagcatt aacgttacaa gaatggcagc aggcagttgt cttaacgcac
      361 gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac gattaaaacg tgcggttgat
      421 gtacataatc ttgcagagct tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg
      481 gatagtggta aacatgatgt cgacttaacc accatcgttt attcaccacc gctgaaggat
      541 aactggcgcg gttttgctgg attcggttat gccgatggac aatttagcga aggaaaaggg
      601 attgttcgcg actggcttgc gggtgttgag tggcggtcac gtaatatctg gctcgaggca
      661 gagtacgctg aacgcgtttt caatcatgag cataaacccg gcgcgcgcct gtctggctgg
      721 tatgatttta atgataactg gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt
      781 ccattacggg caatgaaaaa tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg
      841 tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt tcactgattt ttccgacagt
      901 aaccagcgtc atgaagtctc acttgagggt caggaacgca tctggtcttc accatatttg
      961 attgtcgatt tcctacccag tctgtattac gaacaaaata cagaacacga taccccatac
     1021 tacaacccta taaaaacgtt cgatattgtt ccggcatttg aggcaagcca tttgttatgg
     1081 cgaagctatg aaaatagctg ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa
     1141 aaacattatg gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat tagctggaat
     1201 gacgtgattg atgctggcgc aacgctacgc tgggaaaaac gaccttatga cggtgacaga
     1261 gaacacaact tatacgttga attcgatatg acattcagat tttaaggata aatatgttac
     1321 gtaatggaaa taaatatctc ctgatgctgg tgagtataat tatgctcacc gcgtgcatta
     1381 gccagtcaag aacatcattt ataccgccac aggatcgcga atctttactc gccgagcaac
     1441 cgtggccgca taatggtttt gtagcgattt catggcataa cgttgaagac gaagctgccg
     1501 accagcgttt tatgtcagtg cggacatcag cactgcgtga acaatttgcc tggctgcgcg
     1561 agaacggtta tcaaccggtc agtattgctc aaattcgtga agcacatcga ggaggaaaac
     1621 cgctaccgga aaaagctgta gtgctgactt ttgatgacgg ctaccagagt ttttataccc
     1681 gcgtcttccc aattcttcag gccttccagt ggcctgctgt atgggccccc gtcggcagtt
     1741 gggtcgatac gccagcggat aaacaagtaa aatttggcga tgagttggtc gatcgagaat
     1801 attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc ccggctcgtt gagctcgctt
     1861 ctcatacatg gaattctcac tacggtattc aggctaatgc caccggcagc ttattgcctg
     1921 tatatgtaaa tcgtgcatat tttactgacc acgcacggta tgaaaccgca gcagaatacc
     1981 gggaaagaat tcgtctggat gctgtaaaaa tgacggaata cctgcgtaca aaggttgagg
     2041 taaatccaca cgtttttgtt tggccttatg gcgaagcgaa tggcatagcg atagaggaat
     2101 taaaaaaact cggttatgac atgttcttca cccttgaatc aggtttggca aatgcgtcgc
     2161 aattggattc cattccgcgg gtattaatcg ccaataatcc ctcattaaaa gagtttgccc
     2221 agcaaattat taccgtacag gaaaaatcac cacaacggat aatgcatatc gatcttgatt
     2281 acgtttatga cgaaaacctc cagcaaatgg atcgcaatat tgatgtgcta attcagcggg
     2341 tgaaagatat gcaaatatca accgtgtatt tgcaggcatt tgctgatccc gatggtgatg
     2401 ggctggtcaa agaggtctgg tttccaaatc gtttgctacc aatgaaagca gatattttta
     2461 gtcgggttgc ctggcaatta cgtacccgct caggtgtaaa catctatgcg tggatgccgg
     2521 tattaagctg ggatttagat cccacattaa cgcgagtaaa atacttacca acaggggaga
     2581 aaaaagcaca aattcatcct gaacaatatc accgtctctc tcctttcgat gacagagtca
     2641 gagcacaagt tggcatgtta tatgaagatc ttgccggaca tgctgctttt gatggcatat
     2701 tgttccacga tgatgctttg ctttcagatt atgaagatgc cagtgcaccg gctatcacgg
     2761 cttatcagca agcaggcttt agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat
     2821 ttaaacagtg ggcccgcttt aaaagtcgtg cgttaactga cttcacttta gaacttagtg
     2881 cgcgcgtaaa agccattcgc ggtccacata ttaaaactgc acgaaatatt tttgcacttc
     2941 cggtaataca acctgaaagt gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa
     3001 gctatgactg gaccgctatt atggctatgc cttatctgga aggtgtcgca gaaaaatcgg
     3061 ctgaccaatg gttaatacaa ttgaccaatc aaattaaaaa catccctcag gctaaagaca
     3121 aatctatttt agaattacag gcacaaaact ggcagaaaaa tggtcagcat caggctattt
     3181 cttcgcaaca actcgctcac tggatgagcc tattacaact gaatggagtg aaaaactatg
     3241 gttattatcc cgacaatttt ctgcataacc aacctgaaat agaccttatt cgtcctgagt
     3301 tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg tatcgttttt tatattatgt
     3361 ctggtgttat gcatacccct atgcgtagcg tactttcact ctggtgaact gatgatgagg
     3421 ttcgttttct tctggccgtt ttttatgtcc attatgtgga ttgttggcgg cgtctatttc
     3481 tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa
     3541 gataatccgt ctatctccat tatcattccc tgttttaatg aggagaaaaa cgttgaggaa
     3601 accatacacg ccgctttagc acagcgttat gagaacattg aagttattgc cgtaaatgac
     3661 ggttcaacag ataaaacccg tgccatcctg gatcgcatgg ctgcacaaat tccccatttg
     3721 cgggtcattc atctggcgca aaaccagggg aaagccattg cgcttaaaac cggagctgcc
     3781 gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg
     3841 gcggcatata ttgtggaacc gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat
     3901 cctcgtattc gaacacgttc taccctggtg ggtaaaattc aggttggcga gtattcctca
     3961 attattggtt tgatcaagcg aacccagcgt atctatggaa acgtatttac cgtttccggt
     4021 gttattgccg catttcgtcg cagcgccctg gcagaagtgg gttactggag tgacgatatg
     4081 atcaccgaag atattgatat tagctggaag ctgcagttga atcagtggac gattttttac
     4141 gagccacggg cactgtgctg gatattaatg cctgaaacgt taaaagggct gtggaaacag
     4201 cgcctgcgct gggctcaggg cggtgcagaa gtattcctca aaaatatgac aaggctatgg
     4261 cgcaaagaaa actttcgaat gtggccactg ttttttgaat actgtctgac gacaatatgg
     4321 gcctttacct gcctggtcgg tttcattatt tacgcagtcc aacttgccgg tgtaccgtta
     4381 aatattgaat tgacacatat cgctgcgaca catactgccg gaatattatt gtgtacgtta
     4441 tgtttactgc aatttattgt cagcctgatg atcgagaatc gctatgagca taatctgact
     4501 tcatcgcttt tctggattat ttggttcccg gttattttct ggatgctgag cctggcaacg
     4561 acattggtat catttacacg agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta
     4621 agtcccgatc gcgggattct gagaggttaa tatgaacaat ttaattatta cgacccgaca
     4681 atcgccagta cgtttactgg ttgattatgt tgccacaacc atcttgtgga cattatttgc
     4741 tttgttcata ttcttattcg ccatggatct gctgacgggt tattactggc aaagcgaggc
     4801 cagaagccga cttcagttct attttttgct ggcagtggcg aatgccgtcg tgttaattgt
     4861 ctgggcgctg tacaataagc tgcgttttca aaaacagcag catcatgcag cctaccaata
     4921 tacgccgcaa gaatatgcag agagcttagc aatacctgat gagctctatc agcaactaca
     4981 aaaaagccat aggatgagcg tacacttcac cagccagggg caaataaaaa tggttgtttc
     5041 agaaaaagcg ctagtccggg cataaacacc caaaacaaag cccggttcgc ccgggctctg
     5101 caccgataat acacttaact gtatgcatgc agcgtacgtt ggcaaagtgc cgaacgtacg
     5161 cagtcctctt taccgaaccg gacgatccca accatttcat cttcttcgaa acgttccagc
     5221 gcgtcactta atccggagca cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg
     5281 acgataaccg tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc
     5341 acattctgcg cctcgtcaag aatgacgact gcattttcaa aggtacgtcc acgcatatag
     5401 gcgaacggcg caatttccac cttccctatt tccggtcgca ggcagtactg cataaaggaa
     5461 gcccctaagc gccggaccag cacgtcgtag accgggcgaa aatagggagc aaacttttct
     5521 gcgatatctc caggtaagaa gccaagatct tcatcggctt gcagaactgg acgggtgacg
     5581 ataatcctgt cgacatcctt atgtatcagg gcctctgccg cttttgctgc gctgatccag
     5641 gtttttccgc acccggcttc gcccgtggcg aatatcagct gcttactctc aatagccttc
     5701 agatagtgca attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg
     5761 gccataccaa tggcttctac gccgcccatc tgcacaagcg aggtgaccga ttcttcttca
     5821 cgctgcttat ggctgcgcga atcccgtctc agcacacgtt ttgcctcacg acgagctttg
     5881 atcactgctt tttgtcttcc catggagagc accttgagtt gtttgtattc atcacacgcg
     5941 ccgttggcag cgcgattatg cgcacgaaca tcagagggtt ggcttccttg taagccatag
     6001 tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac cgtttgcggc gtcggtaaca
     6061 cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa gttgctaccg gaggagaaat
     6121 ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc agaacaggac gttaccatcc
     6181 gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact taataattac
     6241 aacagatctc gcattcattg caacaatata ttcacttata tttaaccata aaacaccatt
     6301 tcagtgacat tagtttctac tggaaagatg acagagtgat gacagatatg aaaaaagctg
     6361 tgtgctttca gcaggattcg catcaggcat ctgcgcacga acgccggatg cggcgtgaac
     6421 gccttatccg cctaccgaac ccgcaccgat tgtaggcata agaagacgcg caagcatcgc
     6481 atcaggcatc tgcgcaagaa cgccttatcc gtctataaac attaaacccg caataacgcg
     6541 cttccgagat aatcgttcgc gtccttcact cccggcagcg caaaaaaata accgccgccg
     6601 ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac agtcaggaag
     6661 cctttttcca gatcgtgttg gtaacagaca aacagtagcc ccatatccag ttgcccggag
     6721 ttggtgacgc ccagtgaata actgtagcca cgacgcagca tcaggctgga ctcactctcc
     6781 gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcacctt cccttccgga
     6841 tcgctggcgt aatcgggaac atcatgctca tgctgcattc ccagcggcgc accggtttgc
     6901 ttatcacgac caaaaatcgt ctgctgttct ttcagcggag ttctgtccca aaattccact
     6961 cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc tggctcctgc
     7021 tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct atcgggattg
     7081 gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgcc
     7141 gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc tgagcaaatc tggcgtatgt
     7201 ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg tgttggcgca aatttgtagc
     7261 aacacatcac catgacataa cgccgcatcc agcgagtcgt tgggaaaacg cgtcatcttc
     7321 tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc
     7381 accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg
     7441 agtggtggca ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt cagaaaagca
     7501 aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg ctttatcgct ggcaagcaca
     7561 tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctta
     7621 ccataaaacg gctgtttctc attgcgtgca accggtgaaa gcgtacccgg cgcactttgc
     7681 gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc aatgcctttc
     7741 agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat cttcatactg cataatgctt
     7801 aatccaatcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc
     7861 ctttcagtgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag
     7921 tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact tttgccaaca
     7981 gttccgggtt agctttttgc agttgtggac gcagcagatc gacaattttc tgcgagcctt
     8041 caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctga
     8101 ttttgctggc tgccacttcc tcaatcagtc cggctgcacc gccgaccact tttgaaggtg
     8161 ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa agctggtcag
     8221 cgtactgatc catgcctttg gtggtgttgt cgccaaacaa tgctttttcc agacggtgga
     8281 aaccggtgaa ttttggatca gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc
     8341 tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag tgctggcgcg
     8401 tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg tcggtaaagg
     8461 ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa
     8521 ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt
     8581 tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg tattcgccag
     8641 gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc cgctcttcca
     8701 ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg ttctgaataa
     8761 tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatccgtca
     8821 cggtcacttt gacctgcgtc acatcagcgg cgttagccat aaaagcagaa gaaaacagcg
     8881 cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc
     8941 tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg
     9001 ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg agataaataa
     9061 accagacggc gacttcgctg acgctcggcg cttcctgata gccaaaaaac ccttccatca
     9121 gcgtgccaaa cagcgagtga gttgagagca ccgcactcat atcgaaggcg atttcctgaa
     9181 agtggttcca caatccggct tcatgaaatg cgcgaatggc accagctgcc agccctgcgg
     9241 cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac
     9301 cgccccagta gagcaagaag cctagcacca cggcagtagc aagaccgagc attgcaccca
     9361 gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca
     9421 gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc
     9481 cacgctgcaa tgcgctatcg actgcctgct ccagttgtac tttgacgttg cgcgacactt
     9541 tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt
     9601 caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg aagatcccca
     9661 agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca atccatcggc
     9721 ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc
     9781 cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg
     9841 agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggtaaagaa
     9901 aaatacaagc ggaatgttta taaaatgatg ttgaaatgta aacaaagaga ggtaatgtca
     9961 ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga gccatgtgtt
    10021 cttgccggat gccgcgtaaa cgccgtatcc agccgacaaa accgtcctga ctcaatacat
    10081 caaaacttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc atcctcgatt
    10141 atcaaatcta actttttgcc cggaacataa gaacagcgcg ggtgttggtg gcgtcaaaat
    10201 cgctgaaacg ttccccgaag gccggggcag cagggggcgg cggcgattgg ccgccccctg
    10261 cgcgctccct gcaccattca atttatatgg cgaggcacat taagggagcg caacccgctg
    10321 ctgaagccaa acaaaaccgc cgcaggctaa gtcccttagc tttcctgcaa ccgtgacggc
    10381 ggagccgaat gatagtgcgc atcggcctcg gcaaagcgtt tttgcatcgc cgctgacggc
    10441 gctttaccca gcaaactaaa cactacaatc ccaatactgc cgaagataaa gcccggaata
    10501 atttcgtaca gtcccagcca gccgaactgt ttccagacga taaccgtcag cgccccgatg
    10561 atcatccccg ccagcgcacc gttgcgcgtc atgcgtgacc acatcaccga gaacagcacc
    10621 accggaccaa acgccgcgcc aaagcctgcc cacgcatagc tcactagccc cagcacacgg
    10681 ttttccgggt ttgccgccag cgcaatggcc accagcgcca ccaccagcac catcacacgc
    10741 cctacccaca ccagctcttt ctggctggca tgtttacgca gaaacgcttt gtataaatct
    10801 tcggtaatcg cactggagca caccagcagc tggcaactta aggttgacat taccgccgcc
    10861 agaattgccg acagcagaat cccggcaatc cacgggttaa acagaatttg cgccagttcg
    10921 ataaacacgc gctcagcatt ctggtttacc gcacctgcta ccgccgggtg ctcattaaag
    10981 taagcaatac caaagaagcc gacagctacc gcccctgcca ggcagaggat catccaggtc
    11041 atactgatac tacgcgcatg gacaatgctg tggtgagagt ctgccgccat aaaacgcgcc
    11101 aggatgtgcg gctgcccgaa gtaacccagc ccccaaccca tcagtgagat aatggcgaca
    11161 aagttcagcc ctttgagcat atcaacgttt tcgatgctct tttgtttgat cacttccagc
    11221 gagacaccaa agccaccgac actgataatg acgataaccg gcgtcaggat cagggcaaaa
    11281 atcatcaggc tggcctgtac agtgtcagtc cagctcaccg cgaggaaacc gccaataaag
    11341 gtgtaaagga tcgtcgccgc agctcccgcc cacagagccg tttcgtagct catgccaaag
    11401 gtactttcaa acagacgcgc gcctgccaca atgcccgaag cgcaataaat ggtgaagaac
    11461 agcaaaatca ccagcgcgga gataatgcgc aaaatgcggc ttttatcttc aaagcgcccg
    11521 gtgaaataat ccggcaatgt taaggcgtta ttgttgtatt cggtatgcac acgcaaccgc
    11581 cccgccacca gcttccagtt gatccacgcg cctaatgtca ggccaatggc gatccaactt
    11641 tcggaaatcc cggaaagaaa aaccgcgccc ggcaacccca ttaacagcca gccgctcata
    11701 tccgacgcac ccgccgataa tgccgtcacg aatggcccaa ggctacgacc gcccagaata
    11761 tagtcgtcaa agtttttcgt tgatcgccag gcgataaacc caatcaatat catgccaaag
    11821 atatagacac aaaatgtcac caacatcggt gtgctaatag ccatctaaag tctccaaaaa
    11881 attattatcg gcaatgtcga aacttgccgt tatatctgcc accggaacgg ggtaacagag
    11941 tttatgtttt accagggcga ccgtatcctg ccggaagcgc tggttattca caatccattt
    12001 aacacaccat ttacattaaa ttttagtgct cagcgacact atttttcatc aggttgcact
    12061 ctctcacatt ttttgcggtt gcacctttca aaaatgttaa ctgccgcaga gaaaaagtct
    12121 gagttatttt tttaatccct gtcatatcga tttcttttat taacatttca ttcattttta
    12181 agcttgctac gcatgtcaca tttaacatgg ttgcacaaag ttgcaacatc atggatattt
    12241 cacgataacg ttaagttgca cctttcagaa caacaggagt aatggcatgg gaaccaccac
    12301 catgggggtt aagctggacg acgcgacgcg tgagcgtatt aagtctgccg cgacacgtat
    12361 cgatcgcaca ccacactggt taattaagca ggcgattttt tcttatctcg aacaactgga
    12421 aaacagcgat actctgccgg agctacctgc gctgctttct ggcgctgcca atgagagcga
    12481 tgaagcaccg actccggcag aggaaccaca ccagccattc ctcgactttg ccgagcaaat
    12541 tttgccccag tcggtttccc gcgccgcgat cacagcggcc tatcgccgcc cggaaaccga
    12601 agcggtttct atgctgctgg aacaagcccg cctgccgcag ccagttgctg aacaggcgca
    12661 caaactggcg tatcaactgg ccgataaact gcgtaatcaa aaaaacgcca gtggtcgcgc
    12721 aggtatggtt caggggttat tgcaggagtt ttcgttgtca tcgcaggaag gcgtggcgct
    12781 gatgtgtctg gcggaagcgt tgttgcgtat tcccgacaaa gccacccgtg atgcgttaat
    12841 tcgcgacaaa atcagcaacg gtaactggca atcacacatt ggtcgtagcc cgtctttgtt
    12901 tgttaatgcc gccacctggg ggctgctgtt tactggcaaa ctggtttcca cccataacga
    12961 agccagcctc tcccgctcgc tgaaccgcat tatcggtaag agcggtgaac cgctgatccg
    13021 caaaggtgtg gatatggcga tgcgcctgat gggcgagcag ttcgtcacgg gtgaaaccat
    13081 tgcagaagcg ttagccaatg cccgcaagct ggaagagaaa ggtttccgtt actcttacga
    13141 tatgcttggc gaagctgcac tgactgccgc agatgcacag gcgtatatgg tttcctatca
    13201 gcaggcgatt cacgccattg gtaaagcgtc caatggtcgt ggcatctatg aagggccggg
    13261 catttcaatc aaactgtcgg cgctgcatcc gcgttatagc cgcgcccagt atgaccgggt
    13321 aatggaagag ctttacccgc gtctgaaatc actcaccctg ctggcgcgtc agtacgatat
    13381 tggtattaac atcgatgccg aagaggccga tcgtctggag atctccctcg atctgctgga
    13441 aaaactctgt ttcgagccgg aactggcagg ctggaacggt atcggttttg ttattcaggc
    13501 ttatcaaaaa cgctgcccac tggtgattga ttacctgatt gatctcgcca cccgcagccg
    13561 tcgccgtctg atgattcgcc tggtgaaagg cgcgtactgg gatagtgaaa tcaagcgtgc
    13621 gcagatggac ggccttgaag gctatccggt ttatacccgc aaggtgtata ccgacgtttc
    13681 atatctcgcc tgtgcgaaaa agctgctggc ggtgccgaat ctaatctacc cgcagttcgc
    13741 gacgcacaac gcccatacgc tggcagcgat ttatcaactg gcaggtcaga actactatcc
    13801 gggtcagtac gagttccagt gcctgcatgg tatgggcgag ccactgtatg agcaggtcac
    13861 cgggaaagtt gccgacggca aacttaaccg tccgtgtcgt atttatgctc cggtcggtac
    13921 ccatgaaacg ctgttggcgt atctggtgcg tcgcctgctg gaaaacggtg ctaacacctc
    13981 gtttgttaac cgtattgccg acacctcttt gccactggat gaactggtcg ccgatccggt
    14041 cactgctgta gaaaaactgg cgcaacagga agggcaaact ggattaccgc atccgaaaat
    14101 tcccctgccg cgcgatcttt acggtcacgg gcgcgacaac tcggcagggc tggatctcgc
    14161 taacgaacac cgtctggcct cgctctcctc tgccctgctc aatagtgcac tgcaaaaatg
    14221 gcaggccttg ccaatgctgg aacaaccggt agcggcaggt gagatgtcgc ccgttattaa
    14281 ccctgcggaa ccgaaagata ttgtgggctt tgtgcgtgaa gccacgccgc gtgaagtaga
    14341 acaggcgctg gaaagtgcgg ttaataacgc gccaatctgg tttgccactc ctccggctga
    14401 acgcgcggca attttacatc gcgctgccgt gctaatggaa agccagatgc agcaactgat
    14461 tggtattctg gtgcgtgaag ccggaaaaac cttcagtaac gccattgccg aagtgcgcga
    14521 agcggtcgat tttctccact actacgccgg acaggtgcgg gatgatttcg ctaacgaaac
    14581 ccaccgtcca ttagggcctg tggtgtgtat cagtccgtgg aacttcccgc tggctatttt
    14641 caccgggcag atcgctgccg cactggcggc aggtaacagc gtgctggcaa aaccggcaga
    14701 acaaacgccg ctgattgccg cgcaagggat cgccattttg ctggaagcgg gtgtaccccc
    14761 tggcgtggtg caattgctgc caggtcaggg ggaaaccgtg ggcgcgcaac tgacgggtga
    14821 tgatcgcgtg cgcggggtga tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagcg
    14881 caatatcgcc agccgcctgg acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg
    14941 cggcatgaac gcgatgattg tcgattcttc agcactgacc gaacaagtag taatagatgt
    15001 gctggcctcg gcgttcgaca gtgcgggtca gcgttgttcg gcactgcgcg tgctgtgcct
    15061 gcaagatgag attgccgacc acacgttgaa aatgctgcgc ggcgcaatgg ccgaatgccg
    15121 gatgggtaat ccgggtcgcc tgaccaccga tatcggtccg gtgattgata gtgaagcgaa
    15181 agccaatatt gagcgccata ttcagaccat gcgtagcaaa ggccgtccag tgttccaggc
    15241 ggtgcgggaa aacagtgaag atgcccgtga atggcaaagc ggcacctttg tcgccccgac
    15301 gctgatcgaa ctggatgact ttgccgaatt gcaaaaagag gtctttggtc cggtgctgca
    15361 tgtggtgcgt tataaccgta atcagctgcc ggagctgatc gagcagatta acgcttccgg
    15421 ctatggtctg acgcttggcg tccatacacg tattgatgaa accatcgccc aggtcactgg
    15481 ctcggcccat gtcggtaacc tgtacgttaa ccgtaatatg gtgggcgcag tggttggtgt
    15541 gcagccgttc ggcggcgaag ggttgtccgg taccgggccg aaagcaggcg gtccgctcta
    15601 tctctaccgt ctgctggcga atcgcccgga aagtgcgctg gcagtaacgc tcgcgcgtca
    15661 ggatgcagag tatccggtcg atgcgcagtt gaaagccgca ttgactcagc cgctaaatgc
    15721 actgcgggaa tgggcagcaa atcgtccaga attgcaggcg ttatgtacgc aatatggcga
    15781 gctggcgcag gcaggaacac aacgattgct gccagggccg acgggtgaac gcaacacctg
    15841 gacgctgctg ccgcgtgagc gcgtgttgtg tattgccgat gatgagcagg atgcgctgac
    15901 tcagctcgcc gccgtgctgg cggtgggcag ccaggtactg tggccggatg acgcgctgca
    15961 tcgtcagcta gtaaaggcat tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc
    16021 ggaaaatata accgctcaac cctttgatgc ggtgatcttc cacggtgatt cggatcagct
    16081 tcgcgcattg tgtgaagccg ttgctgcgcg ggatggcgca attgtttcgg tgcagggttt
    16141 tgcccgtggc gaaagcaata tccttctgga acggctgtat atcgaacgtt cgctgagtgt
    16201 gaataccgct gccgctggcg gtaacgccag tttaatgact ataggttaaa caggtagccg
    16261 gaggatgtta caacctcctc cggcatcttt aacgtggtcg aatcccctca ataataatcc
    16321 gctgcacgtt ttcaaccgtt tgattgaaaa atatctcatc gcgcaacgtc gcgcctgtca
    16381 ccgcctccac ctgaggggcg aaatcggcgt aatgttgagt ggaagcccaa atcatgaaaa
    16441 tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt gacccaaccg gcaatcagcg
    16501 ccgatttctc atcaattaat gccttcaaat cgcccgtcag ttcatccatt aacagcggcg
    16561 cgcctgccag catctccata cagaacaggc gcgaagcctg cggataatcg cgtgagactt
    16621 ccagcttcag acggatgtac tctttgatcg ccgccagcgg ggcgaaatct tcacgaaacg
    16681 cttttaacgg tgccagccag atatcgagaa tctgccgcag cacggcaata tacagcgcct
    16741 cttttgacgg gaagtaatac agcagattgg tttttgaaac acccgccaac tctgcgatct
    16801 gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa
    16861 tcgctttttt cttcgcgctt actgcgcgcg aacgtttacc cgttgttttc actgcgcctt
    16921 gcgtcatgcg ctctcccctc ttgtagattg gcaacaggat agcaaaaccc ccgcccggcc
    16981 tcgaccttct gcactctcat cgcgcacaga tgcatgtttt atgtgcaact gttttgaccg
    17041 tttagtccac tttttaccag atattttaga caacacaatg ttaacacatt aataacattc
    17101 actttttaaa aactggcatc cgctttgcaa acaagccatt acgcggctga aaaggatatg
    17161 caggatgcag cgccccgtct gactttcact ttacgagacg aagagaggtt gatgatgaaa
    17221 attggcgtat tcgtacctat tggcaacaac ggctggctca tttcgaccca cgcgccgcag
    17281 tacatgccga cctttgaact gaataaagcc attgtgcaaa aagcggagca ctaccatttc
    17341 gatttcgccc tgtcgatgat caaactgcgt ggctttggcg gcaaaactga gttctgggat
    17401 cacaaccttg agtcgttcac cttgatggcg gggctggcgg ccgtgacctc gcgcattcag
    17461 atttacgcca ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg tatggccgca
    17521 accatcgact ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg ctggcaaaag
    17581 cccgagtatg agcagatggg tatctggcct ggcgatgact atttctcccg tcgttacgac
    17641 tatctcaccg aatatgttca ggtgctgcgc gacctgtggg gcacggggaa aagcgatttt
    17701 aaaggcgatt ttttcaccat gaatgattgt cgcgtcagtc cgcaaccgag tgtccccatg
    17761 aaagtgatct gcgccgggca aagcgacgct ggcatggcgt tctccgccca gtatgccgat
    17821 ttcaacttct gtttcggcaa aggcgtaaat acgcccacgg ctttcgcccc gaccgctgcg
    17881 cggatgaaac aggccgcaga gcaaaccggg cgcgacgttg gttcttatgt gttgtttatg
    17941 gtgattgccg atgaaaccga cgatgccgct cgcgccaaat gggaacacta caaagcgggc
    18001 gcggatgaag aggcgttaag ctggctaacc gaacaaagtc agaaagatac ccgctccggt
    18061 actgatacca acgtccgtca gatggccgat cccacttcgg cggtaaacat caatatgggg
    18121 acgttagtcg gttcttacgc cagtgtcgcg cgcatgttag atgaagtcgc aagcgtgcct
    18181 ggtgccgaag gcgtgctgtt gaccttcgat gattttctgt cgggaatcga aaccttcggc
    18241 gagcgcattc aaccactgat gcagtgccgc gcccatctac ctgcgctgac tcaggaggtg
    18301 gcatgatgac aaccttaacc gctcgaccgg aagccattac cttcgatccg cagcaaagtg
    18361 cgctgatcgt ggtggatatg caaaacgctt atgccacgcc aggcggctac ttagatctcg
    18421 ccgggtttga tgtctcaacc actcgcccgg tcattgccaa cattcaaacc gccgtgaccg
    18481 cagcgcgagc ggcagggatg ctgatcatct ggtttcaaaa tggctgggat gaacagtatg
    18541 tcgaagctgg cggccccggc tcaccgaatt ttcataaatc gaacgccctg aaaaccatgc
    18601 gtaagcagcc gcagctgcag gggaaattgc tggcgaaagg ctcctgggat tatcaactgg
    18661 tggatgaact ggtgccgcag cctggcgata ttgtgctgcc gaagccgcgc tacagcggtt
    18721 tcttcaatac accgctggac agcattttgc gcagccgtgg aatacgccat ctggttttca
    18781 ccggtatcgc taccaacgtc tgcgtcgaat cgacgctacg cgatggcttt tttctggagt
    18841 atttcggcgt ggtgctggaa gacgcaacac accaggcggg gccggaattt gcacagaaag
    18901 ccgcgttgtt caatatcgaa accttttttg gctgggtcag cgacgtcgaa acgttctgcg
    18961 acgcgctttc tcccacatcc tttgctcgta tcgcttaagg agtttaacga tgccaaaatc
    19021 cgtaattatt cccgctggca gcagcgcacc gctggccccc ttcgttcccg gcacgctggc
    19081 tgatggcgtg gtgtatgtct ccggtacgct ggcttttgat caacataata acgtgctgtt
    19141 tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa actatccgca aggtgatcga
    19201 gacggcgggt ggcacgatgg cggatgtgac cttcaacagc atctttatta ccgactggaa
    19261 aaattacgcc gcgattaacg aaatctacgc cgagtttttt ccgggtgata aaccggcgcg
    19321 attctgcatt cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg ccacaattgc
    19381 gcatatcgcc aagtgaggcc gcgatgaaac tttcactctc acctccccct tatgctgatg
    19441 cgcccgtagt ggtgttgatt tcgggtcttg ggggtagcgg cagttactgg ttaccgcaac
    19501 tggcggtgct ggagcaggag tatcaggtag tctgttacga ccagcgcggc accggcaata
    19561 atcccgacac gctggcagaa gattacagta tcgcccagat ggcagcggaa ctgcatcagg
    19621 cgctggtagc cgcagggatt gagcattacg cagtggtcgg ccatgcgctc ggtgcgctgg
    19681 tgggaatgca gctggcgctg gattatcccg cgtcggtaac tgtgctgatc agcgttaacg
    19741 gctggctacg aataaacgcc catacgcgcc gctgttttca ggttcgcgaa cgattactgt
    19801 atagcggcgg cgcgcaggca tgggtggaag cgcagccgtt gttcctctat cccgccaact
    19861 ggatggcggc ccgcgcacct cgccttgagg cagaagacgc gctggcactg gcgcattttc
    19921 agggcaaaaa taatttactg cgtcgactta acgccctcaa acgcgctgac tttagtcacc
    19981 atgcggatcg catccgctgc ccggtgcaaa tcatctgcgc cagtgatgat ctgctggtgc
    20041 catcagcatg ttccagtgag cttcatgccg ccctgcccga tagccagaaa atggtgatgc
    20101 gctatggcgg acacgcctgc aacgtgaccg accccgaaac gtttaatgct cttttactca
    20161 acgggcttgc cagcctgtta catcaccgcg aagccgccct gtaaggaatt gctatgaacg
    20221 aagccgttag cccaggtgcg cttagcaccc tgttcaccga tgcccgcact cacaacggct
    20281 ggcgggagac acccgtcagc gatgagacgt tacgggagct ttatgccctg atgaaatggg
    20341 ggccgacatc agctaactgt tctccggcac ggatcgtgtt tatccgcact gtagaaggaa
    20401 aagaacgtct gcgcccggcg ctttccagcg gcaatctaca aaaaaccctg accgcgcccg
    20461 tcaccgctat cgttgcctgg gacagtgaat tttatgaacg gttaccacta ctgtttcccc
    20521 acggtgatgc ccgcagttgg tttacctcca gcccacaact tgccgaagaa acagcgtttc
    20581 gcaacagctc catgcaggcg gcctatctga tcgtcgcttg ccgtgcgctg ggactggata
    20641 ccggcccgat gtcgggtttt gaccgtcaat acgttgacga cgcttttttt gcaggcagca
    20701 cgctgaagag caatctgctg attaatatcg gctatggcga tagcagcaaa ctttatgcgc
    20761 gcctgccacg tctgtccttt gaagaagcct gcgggctgtt gtaaggagca aactatgaac
    20821 atagttgatc aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc ggtcaatatc
    20881 atcaccacgg acggtccagc cgggcgcgcc gggttcaccg ccagcgccgt ctgcagcgtg
    20941 accgatacgc cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt ctggccggta
    21001 ttcaatgaaa atcgaacgct gtgtgtaaat acactgagcg ccgggcagga gccgctttca
    21061 aatctttttg gcggcaaaac gcccatggaa caccgctttg ccgccgcccg ctggcagacg
    21121 ggtgtgaccg gatgcccgca actggaagag gctctggttt cgtttgattg ccgtatcagc
    21181 caggtggtga gcgtcggcac ccacgacatt ctgttttgcg ccatcgaagc gattcatcgt
    21241 cacgccacac cctacgggct ggtgtggttc gatcgcagtt atcacgcgct gatgcgccct
    21301 gcttgttaac ctcctaaagg agacagctga tggcaatgtt cggttttcct cactggcagt
    21361 taaaatcgac ctctacagag agcggcgtgg tcgcgccgta tgaacgattg ccgtttgctc
    21421 agacggcagt aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg gtgttaatgc
    21481 cgattctgat ggggctggat cccaatcttt ccattttaat gtcggggatc ggcacgttac
    21541 tgtttttctt tattaccggc gggcgcgtcc ccagttatct cggttccagc gcggcttttg
    21601 tcggcgtggt gattgccgcg acgggcttta acggtcaggg cattaacccg aatatcagca
    21661 ttgcgctggg cgggattatc gcctgtgggc tggtttatac cgtcattggc ctggtggtga
    21721 tgaaaattgg tacacgctgg attgaacgac taatgccgcc ggtggtgacg ggtgcggtgg
    21781 tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa aagtgtatcg gcttcggcct
    21841 ttgatagctg gatggcggta atgacggtgc tgtgtattgg gctggtggcg gtgtttaccc
    21901 gtggcatgat ccagcggtta ttgattctcg taggattaat tgtcgcctgt ctgctgtatg
    21961 gcgtgatgac caacgtttta gggctgggaa aagccgtcga ttttactctc gtcagccatg
    22021 ctgcctggtt cggtttgcca catttttcca ctcctgcttt taatggtcag gcaatgatgc
    22081 tgattgcgcc cgtggcggtg attctggtgg cggaaaactt aggtcatctc aaggcagtcg
    22141 ccgggatgac cggacgcaat atggacccgt atatggggcg ggcattcgta ggcgatggtc
    22201 tggcaacgat gctttccggc tctgtcggcg gtagcggcgt gaccacctat gctgaaaata
    22261 tcggtgtgat ggccgtgacc aaagtctact cgacgctggt gtttgttgct gctgccgtca
    22321 tcgccatgct gttgggcttt tcgccgaaat ttggcgcgct gatccatacc attcctgcac
    22381 cggtgattgg tggtgcttca attgttgtgt tcgggctgat tgccgtcgca ggcgcaagga
    22441 tctgggtaca aaaccgtgtc gatttaagcc agaacggtaa tttgattatg gtcgcagtaa
    22501 ccttagtgct gggcgcgggt gattttgctc tcacgctggg cggttttacg ttgggaggga
    22561 tcggtacagc aacctttggc gcgattttac tcaatgcgtt gttaagcaaa aggttggttg
    22621 acgttccacc gccagaagtt gttcatcagg agccataaac agaaaaccgc cggggtaacc
    22681 ggcggtgatg actttttgac gagcttcaga caaaaatgca ttcgtcactg attagtccgg
    22741 ctagttgtca cttttaccgt gactgctctt gccacctttt ttacctgctt cagatgcgcg
    22801 ctgcgggtca ttcttgaaat tccccccgct gtgctgtcca ccttttttac ctgcttctga
    22861 tgctctttcg cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga
    22921 cctccgtctg ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca
    22981 tattttcgct acgtgggact aagcttagtg aactgacgcg ggcagacccg cgtttggtaa
    23041 tatcctgcaa cagcttatta gcgagaatag atatcatctc caataattat ccataagccg
    23101 ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt
    23161 gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac
    23221 gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg
    23281 ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg
    23341 gcacgcgcag tcgctgaggg tgcaagcaaa gtcgatggcg cagaagttgt cgttaagcgc
    23401 gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca
    23461 ccggttgcaa ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc
    23521 cgctttggca acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg
    23581 gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc
    23641 ggtcaggaac aaactattac ttcaacctgg acgacccttg cgcatcacgg catggtaatt
    23701 gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg
    23761 ccgtacggcg caaccaccat cgcaggcggt gacggctcac gccagccaag ccaggaagaa
    23821 ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc
    23881 taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg
    23941 aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg
    24001 ggtacgacga aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa gtgttagtca
    24061 aagcctttgc taagaccgat aaagattcgc ttttttgggg cgaacagacc atcgaacgta
    24121 aaaacgttta accagcgact cccccacttc tcgcggggga gttttctgtt atttcaccgc
    24181 ctcattcaac acgctatcaa acttatccat tgggcagaaa ccgttggcat ctatcgggca
    24241 accgcttaat tccagcgtca cgcgctgcgc aggtgcctgc agggttaacg catcggcatt
    24301 acgtaactgt tccgcactct ggtacacata ttcaattttc atcaaatcgc gattggcttt
    24361 gctgtcatgc caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc
    24421 atgcaactga tacggtttga aatccagcgc cgttaacaga gaggcaatgt tggagtcgtg
    24481 cccaaccaac actgtaattt tcggtgcgct ggtgcgatcg gtgaccagag ctttgtcgat
    24541 ataactgacc agcggtttcg caacattgcg cgccacttcc ggtgaggtaa acaggctgtc
    24601 ctggtagccg tttttaagct tcgacaacac cttccactgc tggtcagatt tgatttctcc
    24661 ccaggccacc tgatccatcg gaaaaccttc gtaatattgc aaagtaaacg catctaccag
    24721 cgagttgccg actttcagcg gcccggaaac accaggttct tgttgatact tggcgctaaa
    24781 ggtatttttg ccatccacca gcgaacactg ttgtttctct ttacaggcag gggaatcttt
    24841 atagttaacg attttttcca gtagttggta gctgtcggta agctggagtt tgctgagctc
    24901 tttctccatt gccgccaccg cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg
    24961 gttaaaggtt gggtccatag ttcccatttt ttcctggtga tgcacaggaa tatcacaccc
    25021 cggaaatgcg ccggtaataa agaactgtgc ggtcgcgaca gtacgttgca gactattggc
    25081 ataggcataa acggtgtccg gcggcgggca ttcccccgat tttaccatcc cctgctctgc
    25141 cagccattca cgcatgtaat ggcccatata cacttcgagc acgccacctt tggtggtgag
    25201 ttgcccaccg gggacgtccc attctggcca tttattcggc gtcgactgct ccagcacact
    25261 gccattgttc gccagcggcg cacgtaagtt atggcggctc atcatgagca cttgctgtag
    25321 ctgatagcct tccggtacgg tttgtgcctg agcgtttgaa gcgattaaaa ctatccctgc
    25381 cacagctgcg gcgattagcg ttttgttcat tcctggcacc tcttttgtta tcaatcacta
    25441 aagagtgtga cagaaatatg acatttttcc gggaacaggc tcgcaaaaca gcttgttgag
    25501 cggctgtttt tatattcatc tttaaaaaca ggtaaggttt gatgcaagct atgtctgctt
    25561 atcccctgcc tcgcgtacac cgctgatgat tacacctgtg gctaaacagg atatcagcgg
    25621 cgttactaat acagaggcaa tagtaccaat aataagagaa gtgaggctaa taacaatggt
    25681 ttttagcaca tgcccttgtc agtaaattgc ataacgattt aaatagtcga gttgcgcatt
    25741 catttcctta ttgtttaact tgtaagcctg agcaaaaagt tcaggaggga ggtaaacgtc
    25801 ataatgattg ccagtgttca ttaaataacc atactcaaga tcttcatctg ttgaaaagga
    25861 ttcattcccc aaggagattg atgccatatt actattaaca ata
//
LOCUS       DAPHDX010000061        25869 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPHDX010000061 DAPHDX010000000
VERSION     DAPHDX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.061"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(168..1643)
                     /gene="ampG"
                     /locus_tag="RH058_002457"
     CDS             complement(168..1643)
                     /gene="ampG"
                     /locus_tag="RH058_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV3138034.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1687..2265)
                     /locus_tag="RH058_002458"
     CDS             complement(1687..2265)
                     /locus_tag="RH058_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3138035.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2570..2887
                     /gene="bolA"
                     /locus_tag="RH058_002459"
     CDS             2570..2887
                     /gene="bolA"
                     /locus_tag="RH058_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV3138036.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3231..4529
                     /gene="tig"
                     /locus_tag="RH058_002460"
     CDS             3231..4529
                     /gene="tig"
                     /locus_tag="RH058_002460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV3138037.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4775..5398
                     /gene="clpP"
                     /locus_tag="RH058_002461"
     CDS             4775..5398
                     /gene="clpP"
                     /locus_tag="RH058_002461"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV3138038.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5524..6798
                     /gene="clpX"
                     /locus_tag="RH058_002462"
     CDS             5524..6798
                     /gene="clpX"
                     /locus_tag="RH058_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV3138039.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6986..9340
                     /gene="lon"
                     /locus_tag="RH058_002463"
     CDS             6986..9340
                     /gene="lon"
                     /locus_tag="RH058_002463"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV3138040.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9549..9821
                     /gene="hupB"
                     /locus_tag="RH058_002464"
     CDS             9549..9821
                     /gene="hupB"
                     /locus_tag="RH058_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV3138041.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10013..11884
                     /gene="ppiD"
                     /locus_tag="RH058_002465"
     CDS             10013..11884
                     /gene="ppiD"
                     /locus_tag="RH058_002465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV3138042.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12035..12406
                     /locus_tag="RH058_002466"
     CDS             12035..12406
                     /locus_tag="RH058_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV3138043.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLLERNLAVLTL"
     gene            12500..12898
                     /gene="fadM"
                     /locus_tag="RH058_002467"
     CDS             12500..12898
                     /gene="fadM"
                     /locus_tag="RH058_002467"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV3138044.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12950..13645)
                     /gene="queC"
                     /locus_tag="RH058_002468"
     CDS             complement(12950..13645)
                     /gene="queC"
                     /locus_tag="RH058_002468"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV3138045.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13710..15410)
                     /locus_tag="RH058_002469"
     CDS             complement(13710..15410)
                     /locus_tag="RH058_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV3138046.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQAVTVNELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15510..16328
                     /gene="cof"
                     /locus_tag="RH058_002470"
     CDS             15510..16328
                     /gene="cof"
                     /locus_tag="RH058_002470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV3138047.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHCDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16481..16939
                     /gene="decR"
                     /locus_tag="RH058_002471"
     CDS             16481..16939
                     /gene="decR"
                     /locus_tag="RH058_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV3138048.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16969..18741
                     /locus_tag="RH058_002472"
     CDS             16969..18741
                     /locus_tag="RH058_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV3138049.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18734..20515
                     /locus_tag="RH058_002473"
     CDS             18734..20515
                     /locus_tag="RH058_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV3138050.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20696..21034
                     /gene="glnK"
                     /locus_tag="RH058_002474"
     CDS             20696..21034
                     /gene="glnK"
                     /locus_tag="RH058_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV3138051.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21064..22350
                     /gene="amtB"
                     /locus_tag="RH058_002475"
     CDS             21064..22350
                     /gene="amtB"
                     /locus_tag="RH058_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV3138052.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22398..23258)
                     /gene="tesB"
                     /locus_tag="RH058_002476"
     CDS             complement(22398..23258)
                     /gene="tesB"
                     /locus_tag="RH058_002476"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV3138053.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23476..24048
                     /locus_tag="RH058_002477"
     CDS             23476..24048
                     /locus_tag="RH058_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3138054.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24079..24390)
                     /locus_tag="RH058_002478"
     CDS             complement(24079..24390)
                     /locus_tag="RH058_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV3138055.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24553..24649
                     /gene="ffs"
                     /locus_tag="RH058_002479"
     ncRNA           24553..24649
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RH058_002479"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24759..25829
                     /locus_tag="RH058_002480"
     CDS             24759..25829
                     /locus_tag="RH058_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3138056.1"
                     /translation="MSLMLSRHGIWYYRKVNVLPCGKRREFRRSLGTRSKTEATKRSL
                     RFADRPWLITETSTTTIDLIQSPNSDLSLSELKLHAEQYVEFKSREVCEREVSSIKRC
                     IDKYFSFTEQVFKRSRAAEFISGLDVAIPTKNKYVKKISGFFRWLKHRTDYDFQNPFE
                     GLLQKDREPVSQKREAYTSAQIRCLENGISTLPDWKIWIILLGRFTGMRANEICQLYH
                     GDILKNEGRWCIRIDDRHDGQVVKTENSKRTIPIHNKLIDIGFLDYVQKQKGRLFPQL
                     TFYKGSYSHYFTRWFSGYRKKLGVPEFHSLRHYIATKLKNAGVVEHYAAAFLGHADNS
                     ITYRRYGKSVAVSQLYDLLEHI"
BASE COUNT         6452 a         6227 c         7067 g         6123 t
ORIGIN      
        1 agactcatta gctgaatcgt gtaagaaaaa atagaattca taaattgcgt caataatttc
       61 caaacgctaa gccgcacaaa agaacaaata ttattaagcg atactgatgt ttatttagcc
      121 gtaataatta cggcgagtga ttactacagc taaataatat ttacagatta cgtcagatgc
      181 gtttttcgta gcgccagata atccagcaaa ccaccaagca cgacgccaga aagggcgact
      241 aaaaccccga cttccagcag agcaggcagg aaagagaagt gcgtcaaatc cagcgcgtcc
      301 atcgtcaaca gcagtaacca cacggccaac aggctgacgc ccgccgccag tgtccacatg
      361 gcaaaggcat aacctgccgg atattcggta cgggagataa agttgtcatt tactcgtgta
      421 tattcaagcg tctggcggca aaccagcagc aaaataagcc ccggtacggc agcggcgacg
      481 gagaatagat agaatgtcga ccagccgtgt gcttcaacaa accaacccgc cacggggccg
      541 acataaactc gccctacagc ggaaagcgct gagagcaggg caaattgagt tgcggaaaat
      601 gacttattac acagcgtcat taacagcgcg acaaaggctg atgtgcccat cccgccacag
      661 aggttttcga aaaagacggc tgcgcccatg ctgtagagat gcttatcagt aatcgacagc
      721 agccagtaac cagcgttaga cgcaccttgt aaaatgccga aaatcagcag cgcccggaac
      781 agcgacaggc gctgcatcaa aatcccaccg tacaatgcgc ccacaatggt cgccagtaag
      841 ccaagcgttt tgttaaccac gcctacttca cccgcatcaa acccgacgcc gcgaatcaaa
      901 aacgtggttg tcaggctcat ggcgaatgcg tcgcccagct tatacagcac gataagaagc
      961 aaaataagcc aggcattatt gcgaccaaag aaatctcgca gaggtgcaac aaccgcttgt
     1021 tccagcgttt ttggcacagg aatggtgtcg gtcggttctg gtgcaagcaa cgtcgcgata
     1081 atacaaggga tcaacagcgc cgccatcaac cagtacatgc cctgccagcc cagccattta
     1141 tctgccagcc acagggctaa gccgccggaa accagcatcc ctaaacggta acccagcacg
     1201 ctgattgccg cgcccgcacc acgttcttct gccgggagca catcggtttt ccacgcatcg
     1261 aagacaatat cctgggaggc cgagcaaaaa gcgatcacca ccgccagtgc cgccatccag
     1321 cggagttggg tgccaggttc gagaaaaccc atcgccgcaa tggcgactaa taacaggatt
     1381 tgcgtggcga gcagccaacc gcgccgccgc ccaaaaaatg ggggcgtgta gcggtccatc
     1441 agcggtgacc agaggaattt aaaaacgtaa gcctggccta ccagagagaa gaaaccaatg
     1501 gttttgagat caatattctc gaccgtcatc caggcctgta aggtgccgga tgtcagggcg
     1561 agcggtagcc cggaagcaaa acccaggatc agcaatatgg ctgaacgcgg ctgttgaaaa
     1621 atacgtaaat attgactgga catgggcgtc tacaggcccg gtttgcaccg ggccagaggg
     1681 cagaaattaa cgcgcgttct gcttgataaa ttcgtgaatg ctggtgtcct gagacatatc
     1741 agcgatggta tcggtcagca cactgttaac cgcatcggcg atatttttgt tggaggcctg
     1801 gaacgcacct tcaacgttgt agctggcacg atagtttttg gtcattttgt tgccattctg
     1861 cgcggtagcg atgatggcga tatccgcttt ggtagcgatg ttgtagcgca cgttgccctg
     1921 ggacacgtca gcatacagtt ggctaacgat gatttgcaga ttaaccgggc cattcggacc
     1981 aaccatgtaa ccacgcgcgg tcatctgttt ttccagcact tcttgcagca ggaaacgcag
     2041 atcgcgggag gcggtcaggg taacgatttg attatcgcgg gtgacttttg ccagcgcctg
     2101 atcggtacgc tgatcggcac cattaatgct tacggtgacg cccatcaggc ttggatcctg
     2161 ctgtggcagt gtaatcgtcg gggaaacttc aatagttgtt ggcggttttg cgcatcctgc
     2221 aagcataaac agagcaacta acgggaagag gatttttttg aacatgttcg ggctctcaga
     2281 gactcttaag cgtgtttggt aaaaattccc gccatcataa cattgccaac ggcgagggga
     2341 agtgggtaag gcatgtaaat tcatcatgtt gacgaaataa tcgcccctgg caaaagaaac
     2401 actgatgcga ggcctgtgtt tcaatcttta aatcagtaaa cttcatacgc ttgacggaaa
     2461 aaccaggacg aaacctaaat atttgttgtt aagctgcaat ggaaacggta aaagcggcta
     2521 gtatttaaag ggatggatga catctcagcg ttgtcggagg agatatttca tgatgatacg
     2581 tgagcggata gaagaaaaat taagggcggc gttccaaccc gtattcctcg aagtagtgga
     2641 tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc cattttaaag ttgtgctggt
     2701 cagcgatcgt tttacgggtg aacgttttct gaatcgtcat cgaatgattt acagtacttt
     2761 agcggaggaa ctctctacta ccgttcatgc gctggctctg catacttata ctattaagga
     2821 gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc tgtcgtggag caggaagcat
     2881 cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt atgttgctaa agattttatg
     2941 aaaaacggcc tgcgggccgt tttgttttgt ctggattttg cgctttttgc ccagcattca
     3001 gacgaaaatt gcccgggaat tgtgaaaaaa tacgcgacag cgcgcaataa ccgttctcga
     3061 ctcataaaag tgatgccgct ataatgccgc gtcctatttg aatgctttcg ggatgattct
     3121 ggtaacaggg aatgtgattg attataagaa catcccggtt ccgcgaagcc aacaacctgt
     3181 gcttgcgggg taagagttga ccgagcactg tgattttttg aggtaacaag atgcaagttt
     3241 cagttgaaac cactcaaggc cttggccgcc gtgtaacgat tactatcgct gctgacagca
     3301 tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt attgacggct
     3361 tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc
     3421 aggacgttct gggtgacctg atgagccgta acttcattga cgccatcatt aaagaaaaaa
     3481 tcaatccggc tggcgcaccg acttatgttc cgggcgaata caagctgggt gaagacttca
     3541 cttactctgt agagtttgaa gtttatccgg aagttgaact gcagggtctg gaagcgatcg
     3601 aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt tgacggcatg ctggatactc
     3661 tgcgtaaaca gcaggcgacc tggaaagaaa aagacggcgc tgttgaagca gaagaccgcg
     3721 taaccatcga cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt aaagcgtctg
     3781 atttcgtact ggcgatgggc cagggtcgta tgatcccggg ctttgaagac ggtatcaaag
     3841 gccacaaagc tggcgaagag ttcaccatcg acgtgacctt cccggaagaa taccacgcag
     3901 aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg
     3961 aactgccgga actgactgca gaattcatca aacgtttcgg cgttgaagat ggttccgtag
     4021 aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga gctgaagagc gccatccgta
     4081 accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc taacgacatc gacgtaccgg
     4141 ctgcgctgat tgacagcgaa atcgacgttc tgcgtcgtca ggctgcacag cgtttcggtg
     4201 gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt cgaagaacag gctaaacgcc
     4261 gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac caacgagctg aaagctgacg
     4321 aagagcgcgt gaaaggcctg atcgaagaga tggcttctgc gtacgaagat ccgaaagaag
     4381 ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa catgcgcaat gttgctctgg
     4441 aagaacaggc tgttgaagct gtactggcga aagcgaaagt gactgaaaaa gaaaccactt
     4501 tcaacgagct gatgaaccag caggcgtaat ttacgcagca taacgcgcta aattcgcaca
     4561 aaggcccgtc accgccaggt ggtgggcttt tttttgtcat gaattttgca tggaaccgtg
     4621 cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt gttatgcttg aaatatggtg
     4681 atgccgtacc cataacacag ggactagctg ataatccgtc cataaggtta caatcggtac
     4741 agcaggtttt ttcaatttta tccaggagac ggaaatgtca tacagcggcg aacgagataa
     4801 ctttgcaccc catatggcgc tggtgccgat ggtcattgaa cagacctcac gcggtgagcg
     4861 ctcttttgat atctattctc gtctacttaa ggaacgcgtc atttttctga ctggccaggt
     4921 tgaagaccac atggctaacc tgattgtggc gcagatgctg ttcctggaag cggaaaaccc
     4981 agaaaaagat atctatctgt acattaactc cccaggcggg gtgatcactg ccgggatgtc
     5041 tatctatgac accatgcagt ttatcaagcc tgatgtcagc accatctgta tgggccaggc
     5101 ggcctcgatg ggcgctttct tgttgaccgc aggggcaaaa ggtaaacgtt tttgcctgcc
     5161 gaattcgcgc gtgatgattc accaaccgtt gggcggctac cagggccagg cgaccgatat
     5221 cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgc atgaatgaac ttatggcgct
     5281 tcatacgggt caatcattag aacagattga acgtgatacc gagcgcgatc gcttcctttc
     5341 cgcccctgaa gcggtggaat acggtctggt cgattcgatt ctgacccatc gtaattgatg
     5401 ccagaggcgc aactgtgccg ctatacttat ccagggcggc acaacgctgt aagcggcttg
     5461 cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa gaacaaagaa gaggttttga
     5521 cccatgacag ataaacgcaa agatggctca ggcaaattgc tgtattgctc tttttgcggc
     5581 aaaagccagc atgaagtgcg caagctgatt gccggtccat ccgtgtatat ctgcgacgaa
     5641 tgtgttgatt tatgtaacga catcattcgc gaagagatta aagaagttgc accgcatcgt
     5701 gaacgcagtg cgctaccgac gccgcatgaa attcgcaacc acctggacga ttacgttatc
     5761 ggccaggaac aggcgaaaaa agtgctggcg gtcgcggtat acaaccatta caaacgtctg
     5821 cgcaacggcg ataccagcaa tggtgtcgag ttgggcaaaa gtaacattct gctgatcggt
     5881 ccgaccggtt ccggtaaaac gctgctggct gaaacgctgg cgcgcctgct ggacgtcccg
     5941 ttcaccatgg ccgacgcaac cacgctgacc gaagccggtt atgtgggcga agacgttgaa
     6001 aacatcattc agaagctgtt gcagaagtgc gattacgacg tacagaaagc gcagcgcggg
     6061 attgtctaca tcgatgaaat cgacaagatt tctcgtaagt cagacaaccc gtccattacc
     6121 cgagacgttt ccggtgaagg cgtacagcag gcactgttga aactgatcga aggtacggta
     6181 gctgctgttc caccgcaagg tgggcgtaaa catccgcagc aggaattctt gcaggttgat
     6241 acctctaaga tcctgtttat ttgtggcggt gcgtttgccg gtctggataa agtgatttcc
     6301 caccgtgtag aaaccggctc cggcattggt tttggcgcga cggtaaaagc gaagtccgac
     6361 aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg aagatctgat caagtttggt
     6421 cttatccctg agtttattgg tcgtctgccg gttgtcgcaa cgttgaatga actgagcgaa
     6481 gaagctctga ttcagatcct caaagagccg aaaaacgccc tgaccaagca gtatcaggcg
     6541 ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg aggcgctgga tgctatcgct
     6601 aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc gttccatcgt agaagccgca
     6661 ctgctcgata ccatgtacga tctgccgtcc atggaagatg tcgaaaaagt ggttatcgac
     6721 gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt atggtaagcc ggaagcgcaa
     6781 caggcatctg gtgaataatt aaccattccc atacaattag ttaaccaaaa aggggggatt
     6841 ttatctcccc tttaattttt cctctattct cggcgttgaa tgtgggggaa acatccccat
     6901 atactgacgt acatgttaat agatggcgtg aagcacagtc gtgtcatctg attacctggc
     6961 ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt tctgaacgca ttgaaatccc
     7021 cgtattgccg ctgcgcgatg tggtggttta tccgcacatg gtcatcccct tatttgtcgg
     7081 gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac catgataaaa aaattatgct
     7141 ggtcgcgcag aaagaagctt caacggatga gccgggtgta aacgatcttt tcaccgtcgg
     7201 gaccgtggcc tctatattgc agatgctgaa actgcctgac ggcaccgtca aagtgctggt
     7261 cgaggggtta cagcgcgcgc gtatttctgc gctctctgac aatggcgaac acttttctgc
     7321 gaaggcggag tatctggagt cgccgaccat tgatgagcgg gaacaggaag tgctggtgcg
     7381 tactgcaatc agccagttcg aaggctacat caagctgaac aaaaaaatcc caccagaagt
     7441 gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg gcggatacca ttgctgcaca
     7501 tatgccgctg aaactggctg acaaacagtc cgttctggag atgtccgacg ttaacgaacg
     7561 tctggaatat ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg ttgagaaacg
     7621 cattcgcaac cgcgttaaaa agcagatgga gaaatcccag cgtgagtact atctgaacga
     7681 gcaaatgaaa gctattcaga aagaactcgg tgaaatggac gacgcgccgg acgaaaacga
     7741 agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag agaaagcgga
     7801 agcagagttg cagaagctga aaatgatgtc tccgatgtcg gcagaagcga ccgtagtgcg
     7861 tggttatatc gactggatgg tacaggtacc gtggaatgcg cgcagcaagg tcaaaaaaga
     7921 cctgcgtcag gcgcaggaaa tccttgatac cgaccattat ggtctggagc gcgtgaaaga
     7981 tcgcatcctt gagtaccttg cggttcaaag ccgtgtcaac aaaatcaagg gaccgatcct
     8041 ctgcctggta gggccgccgg gggtaggtaa aacctctctt ggtcagtcca ttgccaaagc
     8101 caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag cggaaatccg
     8161 tggtcaccgc cgtacttaca tcggttctat gccgggtaaa ctgatccaga aaatggcgaa
     8221 agtgggcgtg aaaaacccgc tgttcctgct cgatgagatc gacaaaatgt cttctgacat
     8281 gcgaggcgat ccggcctctg cactgcttga agtgctggat ccagagcaga acgtagcgtt
     8341 cagcgaccac tacctggaag tggattacga tctcagcgac gtgatgtttg tcgcgacgtc
     8401 gaactccatg aacattccgg caccgctgct ggatcgtatg gaagtgattc gcctctccgg
     8461 ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga agcagattga
     8521 acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta tcggcattat
     8581 tcgttactac acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct ccaaactgtg
     8641 ccgcaaagcg gttaagcagt tactgctcga taagtcatta aaacatatcg aaattaacgg
     8701 cgataacctg catgactacc tcggtgttca gcgtttcgac tatggtcgcg ctgataacga
     8761 aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa gtgggcggtg acttgctgac
     8821 cattgaaacc gcatgtgttc cgggtaaagg caaactgacc tataccggtt cgctcggcga
     8881 agtgatgcag gagtctattc aggcggcgtt aacggtggtt cgtgcgcgtg cggaaaaact
     8941 ggggatcaac cctgattttt acgaaaaacg tgacatccac gtccacgtac cggaaggtgc
     9001 gacgccgaaa gatggtccga gtgccggtat tgctatgtgc accgcgctgg tttcttgcct
     9061 gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt gagatcactc tgcgtggtca
     9121 ggtactgccg atcggtggtt tgaaagaaaa actcctggca gcgcatcgcg gcgggattaa
     9181 aacagtgcta attccgttcg aaaataaacg cgatctggaa gagattcctg acaacgtaat
     9241 tgccgatctg gacattcatc ctgtgaagcg cattgaggaa gttctgactc tggcgctgca
     9301 aaatgaaccg tctggcatgc aggttgtgac tgcaaaatag tgacctcgcg caaaatgcac
     9361 taataaaaac agggctggca ggctaattcg ggcttgccag cctttttttg tctcgctaag
     9421 ttagatggcg gatcgggctt gcccttatta aggggtgttg taaggggatg gctggcctga
     9481 tataactgct gcgcgttcgt accttgaagg attcaagtgc gatataaatt ataaagagga
     9541 agagaagagt gaataaatct caattgatcg acaagattgc tgcaggggct gatatctcta
     9601 aagctgcggc tggccgtgcg ttagatgcta ttattgcttc cgtaactgaa tctctgaaag
     9661 aaggggatga tgtagcactg gtaggttttg gtacttttgc cgttaaagag cgtgctgccc
     9721 gtactggccg caacccgcag accggtaaag agatcaccat cgctgctgct aaagtaccga
     9781 gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta agcgttgtcc ccggtgggga
     9841 tgtgacgaag ttcaagggcg catctactga tgtgcctttt ttatttgtat tcggtgactt
     9901 tctgcgtctt gtgggctgac aattgccccc gtttcttgtc acaataggcc tttgcgcgca
     9961 tcgatacgtt gcgtgaggta cacagtcatc tacagcggag tgttgttaca ccatgatgga
    10021 cagcttacgc acggctgcaa acagtctcgt gctcaagatt attttcggta tcattatcgt
    10081 gtcgttcata ttgaccggcg tgagtggtta cctgattggc ggaggcaata actacgccgc
    10141 aaaagtgaat gaccaggaaa tcagccgtgg gcagttcgag aacgccttta acagcgagcg
    10201 taatcgcatg cagcaacagc tgggcgatca atactccgag ctggcagcga atgaaggcta
    10261 tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc gacgaggcgc tgttggatca
    10321 gtacgctcgt gagctgaaac tgggtatcag tgatgagcag gttaaacagg cgattttcgc
    10381 gaccccagcc ttccaggttg acggcaaatt tgataacagc cgctataacg gtatcctcaa
    10441 ccagatgggg atgaccgccg atcagtacgc ccaggcgctg cgtaaccagc tcactaccca
    10501 acagctgatt aacggtgttg ccggtaccga ttttatgctg aaaggtgaaa ccgacgagct
    10561 ggcggcactg gtcgcgcaac aacgcgtggt gcgtgaggcg actatcgatg ttaacgcgct
    10621 ggcggcgaag cagcctgtga ccgaacagga gattgccagc tactacgaac aaaacaaaaa
    10681 caatttcatg acgccggaac aattccgcgt gagttacatc aagctggatg ccgcaacgat
    10741 gcagcaaccg gttagcgatg cggatatcca gagctactac gatcagcatc aggatcaatt
    10801 cacccagccg cagcgtaccc gctacagcat catccagacc aaaactgaag atgaagcgaa
    10861 agcggtactt gatgagctga ataaaggcgg tgattttgct gcattagcca aagaaaaatc
    10921 tgccgatatt atctctgccc gtaacggcgg cgatatgggt tggttagaag atgccactat
    10981 ccccgacgaa ctgaaaaatg ctggtctgaa agaaaaaggg caactgtctg gtgtcatcaa
    11041 atcttcggtc ggtttcctga ttgtacgtct ggacgacatt cagccagcga aagtgaaatc
    11101 gttagacgaa gtacgtgacg acattgcggc gaaagtgaaa cacgaaaaag ccctcgatgc
    11161 gtactatgcg ctgcagcaga aagtgagcga tgcggcaagc aacgacaccg agtctctggc
    11221 cggtgcagag caagctgccg gcgttaaagc cactcagacg ggttggttta gcaaagataa
    11281 cctgccggaa gagttgaact tcaagccggt tgccgacgct atctttaacg gcggtctggt
    11341 aggtgaaaac ggcgcgccgg gcatcaactc tgacatcatc accgtagacg gcgaccgcgc
    11401 attcgtgctg cgcatcagcg agcacaaacc ggaagcggtg aaaccgttgg cagatgttca
    11461 ggaacaagtt aaggcactgg ttcagcacaa caaagctgaa caacaggcga aagtggatgc
    11521 tgagaaactg ctggttgatt tgaaagccgg caaaggtgcg gaagctatgc aggctgccgg
    11581 tctgaaattt ggcgagccga aaatcttaag ccgttccggt cgtgacccga ttagccaggc
    11641 ggcgtttgca ctgccactgc cagcgaaaga caaaccgagc tacggtatgg cgaccgatat
    11701 gcaaggtaat gtggttctgc tggcgctgga tgaagtgaaa caaggttcaa tgccggaaga
    11761 tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac gcacaaatcg tctttgaagc
    11821 tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt ggcgatgcgc tggaacagca
    11881 ataatcctga agccgcctcg caaaaaaatg cgttgcagct gtaacaagaa aaggtcgctt
    11941 tcgcggcctt ttccatttct gaacattgcc atttgtttac tgttttcaat cccgttaagg
    12001 tgattccact gttaacaaac aaggagaaaa cagtatgaaa cacggaatta aagcactgct
    12061 cattaccctg tccctggcct gtgccggaat gtctcatagc gcgctggcgg cagcttctgt
    12121 ggcgaaaccg acggcggtag aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc
    12181 agtaccggcg aaagccagtg acgaagaagg cacccgggtc agcattaata atgccagcgc
    12241 ggaagagcta gcccgcgcga tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag
    12301 ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat ctaaagcagg tgccggggat
    12361 gggcaattcg ctgctggaac gtaatctggc ggtattaacc ctgtaattaa tttgcatagt
    12421 ggcaattttt tgccagactg aagaggtcat accagttatg acctctgtac ttataacaac
    12481 aacgtaaggt tattgcgcta tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt
    12541 ttaccagcac gtcaacaacg cccgctacct tgaatttctc gaagaagccc gctgggatgg
    12601 gttggaaaat agcgacagtt ttcagtggat gacggcccat aacatcgcct tcgtcgtggt
    12661 caatatcaat attaactatc gtcgcccagc ggtattaagc gacctgttaa ctattaccag
    12721 tcagttgcag caattaaacg gtaaaagcgg catcttaagc caggtcatta cactggagcc
    12781 ggaagggcag gtggtagcgg atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca
    12841 gaaagcatta gctctggaag gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac
    12901 gttttgtggt gccggatgct caagccgcat ccggcgacac ccggaataat tacctcaacc
    12961 cggttttctg cttcatcgct gccatcaccg tcggtttatc ggccagataa tgattcaaac
    13021 cgttggcgcg taaattacat gccgcacaat gaccgcaacc gtcgccttta atgccgttat
    13081 agcaggtcaa cgtttcgtta cggactaaat ccagtttgcc gtaataatct gccagcgccc
    13141 aggtttccgc tttatcaatc cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc
    13201 ccaaactgac ggcatggttt agtgctttca caaactcatc gcggcaatcc gggtagccgg
    13261 agaaatccgt ttcacagacg ccggtaatta cggcttctgc ttttacctga tacgcatata
    13321 ttgccgccag cgtcaggaac aaaatattac gccctgggac aaacgtattc gggatgccat
    13381 cggcttcagg ttcataatca ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca
    13441 gctcgttgag cagcgttaca tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca
    13501 gttcgcgtgc cacgtcgatt tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat
    13561 ggacttcatc atattgttgt aatgcctgca ccagacaggt ggtggaatcc tggcctccac
    13621 tgaacacaac gacagcacgt ttcatagata atcctgcttg aacaataaaa gcgttatggt
    13681 aacgcctgcg attaacccgg accagctatt cattgcgacg gcgcgggaag ccaggcttcg
    13741 gtaaattcaa accagccgcg cggtgtcagt cgcacaccgt tcacgccggg aggggcacta
    13801 atgcgatagt gatagttgaa cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa
    13861 accgctttca gggcattaaa tcggttttcc tcatcaggca ttacttgcac cgcgtccagc
    13921 gtcgattgca gatgtgcata tgctggagcg tcgaaaacat gtggccatag tggatcgcaa
    13981 cgtagccatt gctccagagt atattccggt gcttcgccaa ttaatctgtc gcccatcatg
    14041 aggtctgcgt gtgccaatag ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt
    14101 gtaagttcac agccttccgc tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt
    14161 tctatcggta ggtgataaac cagcgtcaag gttttcggta gtttgacttc atccggtact
    14221 tgccattgcg gaatagtcca gcctggcagt aatgcatgac tggcggtgat caggttttct
    14281 cctacttcta acgtttgtaa taaaccggat tgatgaataa tggagatcac ttttcgcgcc
    14341 tgccagaggg agagtcgggg acttttgcgc aacgttaaat agcaaaaacc taaactgatg
    14401 ccactactga cctggctgac ccgttgcagc tcctccggtt tgccgatggt gatttgcacg
    14461 ggatgccgac agctggttcc caaatctttt tcgaaaagcg gcggagttat ccagtactca
    14521 accgctttaa gcagtggatg acgtaaatgg taataatcat ggctttccag gcgcaccagt
    14581 tccgctgtga attgtgttaa gcgaaaagga cccgtgccga tcagtgggaa ttgcggatgc
    14641 gccagatggc tacaatagct cgccagccgg tgcgcaagcc agtaatcagg gcgatgtaaa
    14701 aagaaggtca gacactgcgg atgggtgact tcaatacgct tcacgctaat aaataattga
    14761 tccagtgctg gcagttgtaa cagcatcaat aatcgctggt gtaagtgtga ggcttttact
    14821 gcatcgccgt tatgccagtg tagggttgaa cgaagataaa agtcccagcg taacccgtca
    14881 gtagaggttt cccagtgatg cgctaaatcg ccaatcgggc gctgggtatt attatcgaag
    14941 cgggtcaggc cggaaaatat ctgtccggcg agatgctgct cggcacggcc gggcaaaaag
    15001 cctggttgta gcggttcgag cgggcgatag tagggaatac gcaacgtggg tgtatcgttt
    15061 tgccattgtc cgcccataaa cggctgtaac agagtgcgca gctcacctgg ggccagttgc
    15121 gccagctcca gcacatcttg ctgctttccg gtttccagtg cctgttccat catcgcattg
    15181 cgtagcgatt ccggcgtgac cagaaagcgt aattgtccgc gctttccgcg tcctgactgc
    15241 gcctgccact ccagccatcc cgcctcctgt gcctgacgca acagcgtacg aacatggcgt
    15301 tcgctgcaaa aacagcgttc ggccagttca ttgacggtga cagcttgcgg ctttccggcg
    15361 gaaggttgcc acagacgttg atactggtta agacggttga gcaatcgcat ataaacccgg
    15421 aacaatatta tttaactatt cactattact tccgtatata tcaggtgata ctcaatcacc
    15481 attaaccgtg tcacagagtg gagaaagaaa tggctcgtct ggcagcattt gatatggatg
    15541 gcactttatt gatgcctgac catcatttag gtgagaaaac cctctctact ttggcgcgac
    15601 tgcgtgaacg cgacattacc ctcacttttg ccacggggcg tcatgcgctg gagatgcagc
    15661 atattctcgg ggcgctatcg ctggatgcat atttgattac cggcaacgga acgcgcgtgc
    15721 attctctgga aggtgaactt ttacattgtg atgatttacc tgcggatgtc gcggagctgg
    15781 tgctgtatca gcaatgggat acccgagcca gcatgcatat cttcaatgac gacggttggt
    15841 ttaccgggaa agagatccct gcgttgttgc aggcatttgt ctatagcggt tttcgttatc
    15901 agataatcga tgtcaaaaaa atgccactcg gcagcgtcac caagatctgc ttctgtggcg
    15961 atcacgacga tcttacacgc ttgcagatcc agctatacga agcattaggc gagcgtgcac
    16021 atttgtgttt ttccgccacg gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg
    16081 ctgcattgac ggtgctgacc caacatttag gtttatcgtt gcgcgattgc atggcctttg
    16141 gtgatgcgat gaacgatcgc gaaatgttag gcagcgtcgg tagcggcttt attatgggca
    16201 atgcgatgcc gcaactgcgc gcggagctcc cgcatttacc ggtgattgga cattgccgaa
    16261 atcaggctgt ctctcactat ttgacgcact ggctggacta tccacatcta ccttattccc
    16321 ccgaataacg agatcccttc cagcaccggg caattgcccg gttttttttg cgttgaattt
    16381 gtcattttgt gccgtggtgt ttaaaccgca cagaataaat tgtcgcgatt tcacctttaa
    16441 aatagaatta aaagagaaaa aaattctccg tggaagggct atgttagata aaattgaccg
    16501 taagctgctg gccttactgc agcaggattg caccctctct ttgcaggcac tggctgaagc
    16561 cgttaatctg acaaccaccc cttgctggaa gcgcctgaaa cggctggagg acgacggtat
    16621 ccttatcggc aaagtcgccc tgctggatcc ggaaaaaata ggcctcggcc tgaccgcctt
    16681 tgtgctgata aaaacgcaac atcacagcag cgaatggtat tgccgctttg tcacggtggt
    16741 taccgaaatg ccagaagtgc tggggttctg gcgcatggct ggtgaatacg attatctgat
    16801 gcgcgtccag gttgccgaca tgaaacgcta cgacgagttt tataagcgtc tggtaaacag
    16861 cgtgccgggg ctgtcggacg tcacttccag cttcgcgatg gaacagatta aatacaccac
    16921 ttctttaccc atcgaataaa tatccagaat caggtcagga cacaacgcgt gcgattattt
    16981 gctcaattaa gctggtattt ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg
    17041 cttgtcatta tcgcgatgct gcaactggtt ccgccaaaag tggttggtat tgttgtcgat
    17101 ggcgtgacag aacaacactt tactaccggg cagatcctga tgtggatcgc caccatggtg
    17161 ctgattgccg ttgtggttta tctcctgcgt tacgtctggc gggtattgct gtttggtgcg
    17221 tcttatcaac tggctgttga actgcgtgaa gattattacc gtcagctaag ccggcagcat
    17281 cctgagtttt acctgcgtca tcgcacgggt gatctcatgg ctcgtgcgac caatgacgtc
    17341 gatcgcgtcg tgtttgccgc cggagaaggg gtgctgacgc tggtggattc actggtgatg
    17401 ggctgcgctg tgttgattat gatgtctacg caaattagct ggcagttgac cttatttgcc
    17461 ctgttgccga tgccagtgat ggcgatcatg atcaagcgca acggcgatgc cttgcatgaa
    17521 cgctttaagc tggcacaggc ggcgttttct agtcttaatg accgcaccca ggaaagcctc
    17581 accagtatcc gcatgatcaa agcctttggt ctggaagatc gccagtcggc gttatttgcc
    17641 gcggatgccg aagataccgg caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc
    17701 gacccaacca tctatatcgc gattggtatg gcgaacttgc tggcgattgg cggcggtagt
    17761 tggatggtgg cgcagggcag tttaacgctg ggccagctca ccagttttat gatgtattta
    17821 ggtctgatga tttggccaat gctggcgctg gcatggatgt ttaacattgt ggaacgtggt
    17881 agtgctgcat acagccgtat tcgcgcgatg ctggcggaag cgccggtggt gaacgatggt
    17941 agcgaacccg tgccggaagg gcgtggcgaa ctggatgtaa atattcacca gttcacgtat
    18001 ccgcagactg accatcctgc gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg
    18061 ctgggtatct gcgggccgac tggttccggc aaaagtaccc tgttgtcgct cattcagcgt
    18121 catttcgacg tcagcgaggg ggatattcgc tttcatgata ttcctctgac gaagttacaa
    18181 ctcgatagct ggcgtagccg cctggcggta gttagccaga caccgttcct tttttccgac
    18241 accgtagcga acaacatcgc gttgggttgc ccgaatgcca cccagcaaga gattgagcat
    18301 gtcgcgcggt tagccagcgt acatgacgat attttgcgtt taccgcaagg ttacgataca
    18361 gaggtgggcg agcgcggcgt gatgctttcc ggcgggcaaa aacagcgtat ctctattgct
    18421 cgtgcgttat tagtcaacgc ggaaatcctc atccttgatg atgcgctttc ggcggtggac
    18481 ggacgcacag agcaccagat cctgcataac ctgcgtcagt ggggacaggg tagaacggta
    18541 atcatcagtg cccatcgcct ttcggcactg acggaagcca gtgaaattat tgtgatgcag
    18601 cacggacata tcgcccagcg tggcaatcat gatgagctgg cgcaacaaag cggctggtat
    18661 cgcgatatgt atcgctatca acaactggag gcggcgctcg acgacgctcc ggaaattcgc
    18721 gaggaggccg tcgatgcgta gttttagcca actgtggccg actctcaagc gtctgttagc
    18781 gtacggttcg ccgtggcgta aaccgctggg gattgcggtc ctgatgatgt gggttgcggc
    18841 agcggcagaa gtcagtgggc cgctgcttat cagctatttt atcgacaata tggtagcgaa
    18901 aaataacctg ccgttgaaaa tggttgcagg gctggctgcg gcgtatgttg ggctacaact
    18961 gtttgccgcc gggctacatt acgcgcagtc gctgctgttt aatcgggcgg cagtaggcgt
    19021 agtgcaacag ttgcgtaccg acgtgatgga tgccgcattg cgccagccat taagcgagtt
    19081 tgatacccaa cccgtcgggc aggtgatttc ccgcgtcact aatgacactg aagtgatccg
    19141 cgatctctac gttaccgtag tggcaactgt cctgcgtagc gccgcactgg tgggcgcgat
    19201 gctggtggcg atgttcagcc tcgactggcg aatggcgctg gtggcgataa tgattttccc
    19261 ggtggtgctg gtggtaatgg tgatatacca gcgttacagc acgccgattg tccgccgtgt
    19321 gcgcgcctat ctagcggata tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt
    19381 tatccagcag ttccgtcagc aggcgcgatt tggcgaacgt atgggggagg ccagtcgttc
    19441 acactatatg gccaggatgc aaaccctgcg cctcgacggt tttctgctgc gtccgctgct
    19501 gagtctgttt tcatcgctca ttctttgtgg cttgttgatg ctgtttggct tctccgctag
    19561 cggcaccatt gaagtgggcg tgctgtatgc gtttatcagc tatcttgggc gacttaacga
    19621 accattaatc gaactgacca cgcaacaggc gatgctgcaa caggctgttg ttgctggtga
    19681 gcgcgtgttt gaactgatgg acggaccgcg ccagcaatat ggcaatgatg agcgcccgtt
    19741 acagagtggc accatcgaag tcgataacgt gtcatttgct tatcgcgatg acaatctggt
    19801 gctaaagaac attaatctct ctgtgccttc gcgtaacttt gtggcactgg tcgggcatac
    19861 cggcagtggc aaaagcaccc tcgccagttt attgatgggc tattacccgc taacggaagg
    19921 tgagattcgc cttgatggtc gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg
    19981 cgtggcaatg gtgcagcaag atccggtggt gctggcggat accttcctcg ccaacgtgac
    20041 gctggggcgt gatatctccg aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc
    20101 ggagctggcg cgcagcatga gtgatggtat ttatacgccg ctgggcgagc aggggaataa
    20161 tctctccgtc gggcaaaagc aactgctggc actggcacgc gtgttggtgg agacgccgca
    20221 aatcctgatc cttgatgagg caaccgccag cattgactcc ggtactgaac aggcgattca
    20281 acatgctctg gcggctgtgc gtgaacatac cacgctggtg gtgattgctc accgcttatc
    20341 gaccattgtt gatgccgaca ccattctggt gcttcatcgt gggcaagccg tggagcaggg
    20401 cactcaccag caactgctgg cggcccaggg acgctactgg cagatgtatc aactgcaact
    20461 tgcgggcgaa gagctggcag ccagcgtgcg tgaagaagaa tcattgagcg cctgaatagc
    20521 gcaatatttc atcgttggtg caaaaatgta acgcactgtg cactgtcata gtgcgttttc
    20581 attttcaatc ttcttaactt cctgctctct ttctcgtttt tcatttctgg cacaccgctt
    20641 gcaatacctt cttcgtgtag cagaaccatt accgaattct gaccggaggg gatctatgaa
    20701 gctggtgacc gtgataatca aaccattcaa gctggaagac gttcgtgaag cgttatcttc
    20761 cattggtatt cagggcctga ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca
    20821 tgccgagctg taccgggggg cggaatacag cgtcaacttc ctgccaaaag taaaaattga
    20881 tgtggcaatt gctgatgacc aactcgatga agtgatcgat atcgtcagta aggcggctta
    20941 caccggaaaa attggcgacg gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat
    21001 tcgtaccggc gaagccgacg aagcggcgct gtaatctctg gcacacagca acaggaacga
    21061 aaaatgaaga tagcgacgat aaaaactggg cttgcttcac tggcgatgct gccgggactg
    21121 gtaatggctg cacctgcggt ggccgataaa gccgacaatg cgtttatgat gatttgtact
    21181 gctctggtgc tgtttatgac tattccgggg attgccctgt tttacggtgg gttgattcgc
    21241 ggcaaaaacg ttctgtcgat gctgacgcag gtgacggtga cattcgcact ggtctgtatt
    21301 ctctgggtgg tttacggtta ctcgctggcg tttggtgagg gcaacaactt cttcggcaac
    21361 attaactggc tgatgctgaa aaacatcgaa ctgacggcgg tgatgggcag catttatcag
    21421 tatatccacg tagcgtttca gggatcgttt gcctgcatta ccgtaggctt gatcgttggg
    21481 gcgctggcgg aacgaatccg cttctcagct gtgttgattt tcgtggtggt atggctgacg
    21541 ctctcttaca ttccaattgc gcatatggtc tggggcggtg gtttgctggc ttctcacggt
    21601 gcgctggatt tcgcgggtgg cacggtggtg cacattaacg ccgcaatcgc cggtctggtg
    21661 ggcgcgtatc tgataggaaa acgcgtgggc ttcggtaaag aggcgtttaa accacacaac
    21721 ctgccgatgg tcttcaccgg gactgccatt ctctatatcg gttggtttgg ctttaacgcc
    21781 gggtcagcgg gcacggcgaa tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca
    21841 acggcggcgg caattcttgg ctggatcttc ggtgaatggg cgctgcgtgg taagccttct
    21901 ctgctgggag cgtgttctgg cgcgattgcc ggtctggtcg gcgtgacgcc agcgtgtggc
    21961 tacattgggg ttggcggcgc gttgattatc ggcgtggtag ctggtctggc gggcttgtgg
    22021 ggcgttacta tgctcaaacg cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg
    22081 cacggcgttt gtggcattgt cggctgtatc atgactggga tttttgccgc cagttcgctg
    22141 ggtggcgtgg gcttcgctga aggtgtgacg atgggccatc agttgctggt acagctggaa
    22201 agcatcgcca ttacgatcgt ctggtccggt gtggtggcat ttattggcta caaactggcg
    22261 gatctgacgg ttggtctgcg tgtaccggaa gagcaggagc gagaagggct ggatgtcaac
    22321 agtcacggcg agaatgccta taacgcgtaa caagcactgc aaaaaatagc cggacggttt
    22381 tacctccggc tattttttta attgtgatta cgcatcaccc cttcctgaac ggtcgaggca
    22441 accagcacgc cgtcctgggt ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc
    22501 gaggtgctct ccacgctata cagcagccat tcattcaaat taaacgggcg atggaaccac
    22561 atggaatggt caatggtggc aatctgaatc cccggttcga gaaaaccgat gccgtgcggc
    22621 tgtagagcta ccggcaggaa gttaagatca gaagcgtaac cgagcagata ctgatgaacg
    22681 cgcaggtcat ccggcacgct accatttgcg cggatccaca cctgacgatg tggttctgcg
    22741 acgtgacctt tcagtgggtt atgaaactcc accggacgga cttccagcgg acgatcgcag
    22801 atgaatttat ctttaagcac tggcggcagc aggtgcgcca gcgattgggc gatttgcgtt
    22861 tccgaaggca ggccatcagg cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc
    22921 gcttctggtg cctggaaaga ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca
    22981 gcaacccggc gggcgctgaa gctgttaccg tcacgcagcg tttcgacatc ataaataatc
    23041 ggcttcttac tatcgccagg gcgaagaaag tagctgtgaa acgaatgtac cagccgctct
    23101 tcagggacgg tctcttttgc agcatacaag gcctgaccca cgacctggcc gccaaacacc
    23161 tggcgtaaac ctaaatcttc actctggccg cgaaagagtc cttcctcaat tttttccaga
    23221 tttaacaatg tcagtaaatt ttttagcgcc tgactcatat aactctccag taacaaagct
    23281 gccgcagcaa gccaaagtga gttgagtata acgcaaattt gctactggtc cgatgggtgc
    23341 aatggtctga attacgggct aattacaggc agaaatgcgt gatgtgtgcc acacttgttg
    23401 acgttactat tttgttaacc actcttccgg cgaggaaagt tagcccgctg gtgcattgat
    23461 aataaggaga aatgaatgaa actcgtgcac atggccagtg gtttagcggt tgcgattgcg
    23521 ttggcggctt gcgcagataa aagcgcggat attcagacgc cagccccggc tgcaaatacg
    23581 tctatttcag caacacaaca acctgctatc cagcaaccga atgtctccgg taccgtctgg
    23641 atccgtcaga aagtcgcact gccgcctgat gctgtgctga ccgtgacact ttctgacgcg
    23701 tcgttagccg atgcaccgtc aaaagtgctg gcgcagaaag cggtgcgtac tgaaggtaaa
    23761 cagtcaccat tcagctttgt tctgccattt aacccggcag atgttcagcc gaacgcgcgt
    23821 attctgttga gtgcggcgat taccgtgaat gacaaactgg tatttattac cgataccgtt
    23881 cagccggtga tcaacaaggg cggaactaaa gccgacctga cattggtgcc ggtgcagcaa
    23941 accgccgtgc cggttcaggc cagcggtggt gcaacgacta ccgtaccttc gacttcacca
    24001 actcaggtga atccgtcttc ggcagttcca gctcctacgc aatattaagc gacgttaacc
    24061 ctctccgccc ggagagggtt agtagttcca gcgataacgc tgcaagtcga tttgcccgct
    24121 tcccgatacc atcacacctt ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc
    24181 ggttagcgaa attgtgccgt ggcgattaac cacccggtgc cagggtaagg tgctgccttc
    24241 agggagacgc tttaacacac cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt
    24301 cgccacatca ccgtaagtgg tgacatagcc ttcgggaata gcggcgacga tttgccagac
    24361 gcgttgggga aatgaatctt ctttttccat cttttcttcc tgaggtaatt tttcagcata
    24421 atctggaaaa acgcccgagt gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat
    24481 gggttgcaat tagccggggc agcagtgata atgcgcctgc gcgttggttc tcaacgctct
    24541 caatgggggc tctgttggtt ctcccgcaac gctactctgt ttaccaggtc aggtccggaa
    24601 ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc ccccacccat
    24661 ttcagggtaa gtacttgata gataatgtct aaagtactga aatctctccc ccatagcctt
    24721 agtggtacaa aaactgtgta tcaccagtag gctgcacgat gtcccttatg ctctcccgtc
    24781 atgggatctg gtactaccgg aaagttaacg tcttaccctg tggtaaacgc cgtgagtttc
    24841 gtcgttcgtt aggtactcgt tcaaagactg aagcgacaaa gcgatcttta cgctttgcag
    24901 atcgcccttg gctgattact gagacgtcca cgactacaat cgatttgatc caatccccta
    24961 attcagactt gtcgcttagc gagctaaaac tacatgctga gcagtacgtt gagtttaaaa
    25021 gccgtgaagt ttgtgaaagg gaagtttctt ctatcaaacg ctgtattgat aagtacttta
    25081 gcttcactga gcaggtgttt aagcgtagta gggctgctga attcattagc gggctggatg
    25141 tcgcgattcc tacaaagaac aaatatgtta agaaaatcag tgggtttttt cgatggctga
    25201 aacaccgtac tgactatgat tttcaaaatc cctttgaggg attgctccaa aaggatcgcg
    25261 agccagtttc ccaaaagcga gaggcatata cctctgctca gatcagatgc ctagagaatg
    25321 gaatcagtac attacctgac tggaagatat ggattatttt gcttggtcga tttacgggga
    25381 tgagggctaa tgaaatttgt caactatatc atggtgatat attaaaaaat gagggaagat
    25441 ggtgcatcag gattgatgat cgtcatgatg gacaggtggt caaaacggag aacagtaaac
    25501 gtacaatacc aattcataat aaattgatag atattggatt tttggactat gtacagaaac
    25561 agaaaggcag actgtttccg cagttaactt tttataaagg gtcatacagc cactacttca
    25621 cccgctggtt cagcggttac cgcaaaaaat taggggttcc agagtttcat tcactgcgac
    25681 attatattgc cactaagtta aaaaatgctg gtgtagtgga gcattatgct gctgcattct
    25741 taggacatgc agataattca attacataca gaagatatgg taagtcagtc gcggtatctc
    25801 aactatatga tctattagaa catatttgat tcgtactaag cagcctcttc gctgagagcc
    25861 tgcttagtg
//
LOCUS       DAPHDX010000062        25488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPHDX010000062 DAPHDX010000000
VERSION     DAPHDX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.062"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..396
                     /locus_tag="RH058_002481"
     CDS             7..396
                     /locus_tag="RH058_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138057.1"
                     /translation="MKRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            461..1519
                     /locus_tag="RH058_002482"
     CDS             461..1519
                     /locus_tag="RH058_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3138058.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            1560..2282
                     /locus_tag="RH058_002483"
     CDS             1560..2282
                     /locus_tag="RH058_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HDV3138059.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSMLFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            2279..3100
                     /locus_tag="RH058_002484"
     CDS             2279..3100
                     /locus_tag="RH058_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDV3138060.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            3075..3332
                     /locus_tag="RH058_002485"
     CDS             3075..3332
                     /locus_tag="RH058_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3138061.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            3344..3595
                     /locus_tag="RH058_002486"
     CDS             3344..3595
                     /locus_tag="RH058_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3138062.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            3600..4181
                     /locus_tag="RH058_002487"
     CDS             3600..4181
                     /locus_tag="RH058_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138063.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            4178..5536
                     /locus_tag="RH058_002488"
     CDS             4178..5536
                     /locus_tag="RH058_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDV3138064.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            5523..5876
                     /locus_tag="RH058_002489"
     CDS             5523..5876
                     /locus_tag="RH058_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HDV3138065.1"
                     /translation="MIRHEIERHQVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            5867..7543
                     /locus_tag="RH058_002490"
     CDS             5867..7543
                     /locus_tag="RH058_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3138066.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7547..7969
                     /locus_tag="RH058_002491"
     CDS             7547..7969
                     /locus_tag="RH058_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDV3138067.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            7966..8571
                     /locus_tag="RH058_002492"
     CDS             7966..8571
                     /locus_tag="RH058_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HDV3138068.1"
                     /translation="MKFLPLLVLLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDHTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            8540..10858
                     /locus_tag="RH058_002493"
     CDS             8540..10858
                     /locus_tag="RH058_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HDV3138069.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10855..11439
                     /locus_tag="RH058_002494"
     CDS             10855..11439
                     /locus_tag="RH058_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HDV3138070.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            11441..12610
                     /locus_tag="RH058_002495"
     CDS             11441..12610
                     /locus_tag="RH058_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HDV3138071.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12607..13071
                     /locus_tag="RH058_002496"
     CDS             12607..13071
                     /locus_tag="RH058_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HDV3138072.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            13071..13802
                     /gene="fabG"
                     /locus_tag="RH058_002497"
     CDS             13071..13802
                     /gene="fabG"
                     /locus_tag="RH058_002497"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV3138073.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGSRQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13799..15028
                     /locus_tag="RH058_002498"
     CDS             13799..15028
                     /locus_tag="RH058_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDV3138074.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(15868..16899)
                     /locus_tag="RH058_002499"
     CDS             complement(15868..16899)
                     /locus_tag="RH058_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3138075.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            17170..17613
                     /locus_tag="RH058_002500"
     CDS             17170..17613
                     /locus_tag="RH058_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3138076.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            17629..17916
                     /locus_tag="RH058_002501"
     CDS             17629..17916
                     /locus_tag="RH058_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3138077.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            17929..19185
                     /locus_tag="RH058_002502"
     CDS             17929..19185
                     /locus_tag="RH058_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV3138078.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(19401..19685)
                     /locus_tag="RH058_002503"
     CDS             complement(19401..19685)
                     /locus_tag="RH058_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3138079.1"
                     /translation="MEQKTLSVEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(20107..21120)
                     /locus_tag="RH058_002504"
     CDS             complement(20107..21120)
                     /locus_tag="RH058_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDV3138080.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTEQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(21132..22448)
                     /gene="fucP"
                     /locus_tag="RH058_002505"
     CDS             complement(21132..22448)
                     /gene="fucP"
                     /locus_tag="RH058_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV3138081.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(22476..23396)
                     /gene="rbsK"
                     /locus_tag="RH058_002506"
     CDS             complement(22476..23396)
                     /gene="rbsK"
                     /locus_tag="RH058_002506"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV3138082.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            23699..24481
                     /locus_tag="RH058_002507"
     CDS             23699..24481
                     /locus_tag="RH058_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV3138083.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(24483..24709)
                     /locus_tag="RH058_002508"
                     /pseudo
     CDS             complement(24483..24709)
                     /locus_tag="RH058_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         6247 a         6489 c         6401 g         6351 t
ORIGIN      
        1 gcactaatga aacgttatat aaaatggttt gccatcacaa tttttatcag tatgttgagt
       61 gcctgtgccc gtacggcccc agtgcaacag ataagcacca ctgtcagtgt gggtcatact
      121 caggagcagg ttaaaaatgc cattttgaaa gcaggtgcgc agcgcaagtg gattatgacg
      181 caagtgtccc ctggagttat taaagctcgc tatcaaacac gaaatcacgt tgcagaggtt
      241 cgtattacat atacagctac ctactataac atcaaatatg acagtagcct gaatctgcag
      301 gcttctgatg gaaaaattca taaaaactat aaccgctggg tgcgtaacct agataaagat
      361 atacaggtta acttatctac aggagcaacg ttatagtgat gctgatatcg ggctggaatg
      421 gcccgatagt atttaacttt ctctataaga atggttatat atgtacgaac aggacacgct
      481 cagcgcactg gatgccatca ccgaagcaca gcggattgcc tttgctccta tgcttttcca
      541 ggcagctttg tgcctgcgca atgcaggaat attagattat ttggatcaac agggtaaaca
      601 gggagctccc cttaatgcga ttactgagca cacagtcctg aatgaatatg ccgtgggtgt
      661 gttactggat atgggcttaa gcgggcgaat tattacctgt aaagagggaa tttattacct
      721 ggcgaaaatc ggtcattacc tcctgcatga cactatgact cgcgtgaata tggatttcac
      781 ccaggatgtc tgttaccagg ggctcttctt ccttgccgat tcactcaatg aaggaaaacc
      841 ttccgggtta aaagtatttg gcgactggcc gacgatatat ccggcacttt cgcaattacc
      901 agacgccgca cgtgatagct ggtttgcgtt tgatcattac tattcagatg gcgcatttaa
      961 cgccgcactg ccttatgtat tcgccaacaa ccccacaacc ttgtacgatg tgggtggcaa
     1021 tacgggtaag tgggctttgc gctgctgcaa attcaatgaa aacatcgccg ttacgttatt
     1081 agatctgcca caacaaatcg tactggcgaa agaaaatatc gcgaatgcag gtttttctga
     1141 tcgcatagat ttccatgcgg tggatatgct aagcgatgcc ccattaccag gcgaggctga
     1201 tatctggtgg atgagccaat ttttagactg tttttcaccc gaacaaataa ttagcatact
     1261 cagcaaaatt gccagcgtaa tgaagcctgg cgcaaaactt tgcatcatgg agttgttctg
     1321 ggatgcacag cggtttgaag cgacatcgtt tagcctgaat gcatcctcac tttattttac
     1381 ctgcatggca aacggtaaca gccgtttcta tagtgcagag aaattttacg actatctgaa
     1441 taaagcagga ttccaggtag cagagcgtca cgataattta ggcgttgggc atacactttt
     1501 gatatgccaa aagaaataat cgtcacttaa taattttggt ttaccgcggg cgcgcgttga
     1561 tgaaatttgc attaaatatt accaactggc aggcactggc gcctggactt agcgatgtgc
     1621 agcaatggca agcatggtcg cgccagccgt gggcgattga ccctgccgcg ccgttggcaa
     1681 aattaagtga actgccaatg atgaccgctc gtcgtctcag ttcaggcagc aaactggctg
     1741 ttgaatgcgg tctgacaatg ctacgccgcc atcagccaga cgctgttttg tataccagtc
     1801 gtcatggcga actggagcgc aattaccgca ttgttcatgc actggcaacc gaacaggcgc
     1861 tttctccgac ggacttcgcg ctttccgtac acaactcttc cgtgggtaac ctgaccattg
     1921 tggccaaaca gccgatcgtt tcgtcatcgc tttcggctgg tcgcgacagt ttccagcaag
     1981 gtttgtgtga agtgcttagc ctgctacaag cgggatacca gcgcgtgttg atggtcgatt
     2041 ttgatggctt tctacctgag ttttatcacc cgcaactgcc cgcagaaatg cctacctggc
     2101 cgtacgccgt cgcgctggtg attgaagctg gcgatgactg gcaatgtgaa acgcagccag
     2161 ccattgcggt aaatgaaaca acactgccgc aaagcatgtt gtttttacag cactatttac
     2221 aaaatgcaga tgctttttca ctgcctggcg agcgcgtgca atggcgctgg agccgcagat
     2281 gagtaagctg atgtcacgtc tggagtggac ctggcgactg gtcatgaccg ggttatgttt
     2341 tgtcctgttt ggtctcggcg gcctgctgct ttcagtcgtg tggtttaaca tattgttagt
     2401 tttggtgtgg gatacttccc gccgccgtcg tttggcacgt cgcagcattg ccgccagttt
     2461 tcgcctgttt ttaactgtcg cgaaagggct tggcgtgctg gattatcgta ttgatggcgc
     2521 tgaaatttta cgtcaggaac gcggttgcct cgtcgtagcc aatcatccca cgctgatcga
     2581 ttacgtgctg ctggcatcag tcatgccaga aactgactgc ctggtgaaaa gcgcgctgtt
     2641 aaaaaatcct tttcttggcg gcgtcgtgcg ggcggcagat tatttaatca acagcgaagc
     2701 tgaaaccctg ttaccccgct gccaacagcg actggcacag ggtgacacta tcctgatttt
     2761 ccccgaaggc acacgaacga agcccggtga aaaaatgacg ttacagcgcg gtgcagcgaa
     2821 tattgccgtg cgctgcacca gcgatttacg catcgtgacg atcggctgta gcgagcatct
     2881 gctggataaa caaagtaagt ggtatgacgt cccgcctgcc aggccgtttt ttacggttga
     2941 agtccatggg cgggtagaaa tcaataaatt ttacgatgca acttcacaag agccgacgct
     3001 ggctgcaaga cagttaaacc ggcatctttt gcttcaatta cagcaaggtt gtttacctct
     3061 atcaggaata aataatgcaa gcgctttatc tggaaattaa aaacctcatc atcagcaccc
     3121 tcaatctgga cgacttgact cctgatgata ttgatatcaa tgcgccactg tttggcgacg
     3181 gtttagggct ggactctatc gatgcactgg aactgggact ggcagtaaaa aatgagtacg
     3241 gcatcgtgct ctctgcggaa agcgaagaaa tgcgtcagca ctttttctcc gtcgccactc
     3301 tggcctcttt cattgctgcg caacgtgcct gaggaaaacc atcatgacag accaacaaac
     3361 catttatcag gaagtctcag ccctgctggt taagctgttt gaaatcgatc cgcaggacat
     3421 caaacctgaa gcgcgactgt acgaagatct ggaactggac agcatcgacg ccgttgacat
     3481 gattgtgcac ctgcaaaaga aaaccggtaa gaaaatcaag ccggaagagt ttaaagcagt
     3541 gcgtacagtc caggatgttg tcgaggctgt agaacgcctg ctacaagaag gctaatttga
     3601 tgtcgggcat tcgctcgcta ccgatgatta agttgctgac cggattattg ctgctcgcct
     3661 ggcctttttt aatctggcta ggtttggtgc ataacagcct gcactggctg ttgccgctga
     3721 tggcgctgct gcttttattg cgcctgcgcc agccccgtcg ccagacgggg ccgctccagg
     3781 tcgtcacaaa aatcgttgct gttgtgggca ttgtattgtg tgtctccagt tttctgttga
     3841 aaacgcacca gttactgctg ttttacccgg tagtggttaa cgcagtcatg ctggcggttt
     3901 ttggcggttc gctgcggtcg acaatgccga tagttgagcg gctggcacgt ttgcaggaac
     3961 cagacctccc ggaaaaggcc gtgcgatata cccgccgcgt aacgcaggtc tggtgcctgt
     4021 tttttatttt caacggcagt attgcgttat ttaccacctt gtatggcaac atgcctttgt
     4081 ggacagcctg gaacggtatc attgcttacc ttctgatagg tttcctgact gcaggggaat
     4141 ggctgatacg ttgtcagatg atcaaacgga aaacaccatg aatcagactc tctcactttc
     4201 acagtggttg acagcctctc gccctgtaac aactcctgtt gcctggcttg gggaatatac
     4261 ctggacactg gggcatctgc ggcatgatgt agcgcttttg atcgaccatt tacgcgacca
     4321 gccaggcaat cgctgggcac tttgttttga aaacagctat ctttttattg tagcgttgtt
     4381 ggccacgcta cactcaggta aaatcccggt aatcccgggc cataatcgcg ccgctttgtt
     4441 aaatgtacaa cgctccttgt ttgattgcgt cctgagtgat aaaaggctgg gctggaacgg
     4501 tccacagttt gttgtaagca catctcacca gatgacgacc agcacgatag cctttgatga
     4561 tattgcttct gacgcgttta ttgaactttt cacatcgggt tcaaccggac aaccaaaaca
     4621 aattgcaaaa cctgttatca gccttgatca agaagcaaac atgctggcaa ctcactttgc
     4681 agaccgacta ctgggctgtc gttttgtagc atccgtgatg cctcagcacc tgtatgggct
     4741 gactttccgt atttttttac ctatggcact tggcctgcca ttgcatgccg caatgctctg
     4801 gtattctgag caacttgccg cattaagcca tacctaccgc tatgcctttg tcagcagccc
     4861 agcgttcctt aaacgactcg accttcatct gacgcctccc cccgtggaaa tgattctctc
     4921 tgccggcggt atgctaccat ggcaggatgt agagaaaact tccacctggc tgaatatctg
     4981 gcccgatgaa atctatggta gtaccgaaac aggtattctc gcatggcgtt accgtcaaca
     5041 ggataatatc ccctggttta ctttttctga tgttcatctt tcacaggaaa gtgagggggt
     5101 tcgtgtcttt tcgccactca tccctgctga aggattggtg cttgacgata tgttgcaatt
     5161 caatgaaaac ggtcagttcc acctgatcgg acgacgtggt cgagtggtaa aaatagagga
     5221 gaagcggata tcactggttg aaatcgaaca acggcttctg gcacttgacg gtattatgga
     5281 tgtcgctgca ataccattaa tacgcaatgg ccgccaggct atcggtgtcg tggttgtacc
     5341 aaataaagag gcccgtcaaa tatggcaatg ctccggaggg aaaactctgg agttgtcctg
     5401 gcgcaaagca ctattaccat ggcttgaacc tgttgcagta cctcgttact ggcgcattat
     5461 tgatgaaata ccagtaaaca atatgaataa gcgtgtctat gcacaattac aggagttatt
     5521 tcatgatacg ccatgaaatt gagcgccatc aggttcaacc gcaggagatg gaaatcgttc
     5581 tataccttga cccgatgctc tactggttta atggtcattt tgccgtacag cctctgttac
     5641 caggcgtggc ccagttggac tgggtaatgc attatgctac cacgctgctt gctccgggct
     5701 ggcgttttcg tagtatccag aatgttaaat ttctggcgcc acttatacct gagacgactg
     5761 ttaccctgca attaacatgg caggaaacaa gtcaggtact cactttctgc tatcagcgtc
     5821 acgacggtga cgcccgccac accgccagta gcgggaagat ccgcctatgt cggtaaactt
     5881 ttctccctgc gtgttgatcc cctgctacaa ccacggcgcg atgatgccgg gcgtgctggc
     5941 gcgtcttaag ccatttaatc ttccctgtat tgtggtggat gacggcagcg atgccgccac
     6001 gcaacagcaa ctggataatc tggttgccga acagcctggc gtgaccttaa ttcgcctggc
     6061 agaaaacaca ggcaaaggcg cggcggtaat gcgcggctta caggcggcgg cagacgcggg
     6121 cttcagccat gcggtgcagg tggatgctga cggtcaacat gcgattgaag atatccccaa
     6181 actgctggca ctcgctgaac aacaccctgt ggcgctgatc tccggccagc caatttacga
     6241 tgactccatc ccccgctcac ggctttacgg gcgctgggtc acccacgtct gggtatggat
     6301 cgaaacgctc tccctgcaac tgaaagacag tatgtgcggc tttcgcgttt atccggttgc
     6361 gccaacgctg caactggcaa aatacgccac catcggcaag cggatggatt tcgacactga
     6421 agtgatggtg cgcctctact ggcagggaaa caccagttat ttcgtgccaa cccgcgtcac
     6481 ttatccgcag gacgggcttt cgcattttga tgccctgaaa gataacgtcc gcatctcgct
     6541 catgcacacg cgtctgtttt tcggcatgtt gccgcgtatt ccttcactac tgatgcgccg
     6601 ctcttccagc cactgggcgc ggcagagtga agtgaaagga ttatggggaa tgcgcctgat
     6661 gctgctggtc tggcgtctgc tgggaagaac ggcgtttagc gcgctgcttt acccggtggt
     6721 gggcgtctac tggctcactg ctgctcgtgc gcgcaaagcg tcgcaagact ggctcgcccg
     6781 tgtacgacag catcaaccac aggcggcaaa actcaacagc tatcagcact ttctacgttt
     6841 cggtaatgcc atgctcgaca aaatcgccag ctggcgcggc gagctacaac tggggcacga
     6901 tgtgctgttt gcgccaggcg cagaagcggc gcttgacgtc cgcgatccgc gcggcaaatt
     6961 gctgctggcc tcgcatcttg gcgatgtgga agtgtgccgg gcgctggcaa aaattcaggg
     7021 ctacaaaacc attaacgcgc tggtgtttag cgaaaacgcc caacgcttta aacagataat
     7081 gcaggagatg gctcctcagg caggcattaa cctgatgccg gtaacagata tcggcccgga
     7141 aaccgccatc ctgctgaaag agaagctgga taacggcgaa tgggtggcga ttgtcggtga
     7201 ccgcatcgcc gtcaccccgc aacgcggcgg cgactggcgc gtctgctgga gtccgtttat
     7261 gggccagcct gcgcctttcc cacaggggcc gtttattctc gcgtctattt tgcgctgccc
     7321 ggtgaatctg attttcgccc tgcgccagcg cggcaagctg catattcact gcgaaacctt
     7381 tgccgacccg ctgctgttgc cgcgtggcga acgccaacag gcgctgcaaa acgctatcga
     7441 ccactacgcc gcgcgtctgg aacattacgc gctccagtcg cccctcgact ggtttaattt
     7501 tttcgatttc tggcaactgc cggaaattca ggacaaggag taaagggtgc ttaacgatcc
     7561 ccgatttacc gctgaagtcg agctgaccat tccctttcac gacgtcgata tgatgggcgt
     7621 ggcctggcac ggtaactatt ttcgctactt tgaagtggcc cgcgaggcgc tgctcaacca
     7681 gttcaattac ggctatcggc agatgaaaga gtcaggttat ttgtggccag tggtcgatgc
     7741 gcgggtgaaa tatcgccacg ccctcacctt tgagcaacgc atccgcgtgc gtgcgcatat
     7801 cgaagagttc gaaaaccgtc tgcggattgg ctatcagatt ttcgatgccg aaacgggcaa
     7861 acgcgccacc accggataca ccattcaggt cgcagtagag gagcaaagcc gtgaactgtg
     7921 ctttgtcagc cccgatattc tgtttgaacg catgggagtc aaaccatgaa atttttaccg
     7981 ctgctggtgc tgctgattag cccgtttgtg agcgccctga ccctggacga tcttcagcaa
     8041 cgctttaccg aacaaccggt gatccgcgcc catttcgatc aaacccggac gattaaagat
     8101 ctgccgcagc cgctgcgatc tcagggtcag atgttgatcg cccgcgacca ggggttattg
     8161 tgggatcaaa cctcaccgtt ccccatgcaa ctattactgg acgataaacg catggtgcag
     8221 gtgatcaacg gtcagccgcc gcaaatcatc acggcagaaa acaacccgca gatgttccag
     8281 tttaaccacc tgctgcgcgc gctgttccag gccgatcgca aagtgctgga acaaaacttc
     8341 cgcgtcgaat ttgccgacaa aggcgaaggc cgctggacgc tgcgcctgac gccgaccacc
     8401 acgccgctgg acaaaatttt caacaccatc gatctcgccg ggcaaaccta tctggagagc
     8461 attcagctta atgataaaca gggcgatcac accgatatcg ctcttaccca acatcaactg
     8521 acaccagcgc aactgaccga tgacgaacgc caacgctttg ccacccagta aacgccccgc
     8581 gctgttatgg gggctagtct gcctggtcat ggcggcggcg ttgctgatcc tgctgccaca
     8641 atcacgactg aacagtagcg tgctggctat gttacccaaa caggcgatgg gcgatattcc
     8701 tccggcgctg aatgacggct ttatgcagcg tctggaccgc caactggtgt ggctggtcag
     8761 ccccggtaaa gaggctaatc ccagggtggc tcaggagtgg ctgacgctgc tgcaaaaatc
     8821 cgctgcgctc ggcgacatta aagggccaat ggatgccgcc agccagcagg cgtggggagc
     8881 gtttttctgg cagcaccgca acggcctgat tgaccccgac acccgcgccc gcctgcaaaa
     8941 tggcggcgaa gcgcaggcga agtggatcct ctcccagctt tattctgcgt tctccggggt
     9001 aagcggcaag gagctgcaaa acgatccgct gatgttaatg cgcggctcgc aactggcgat
     9061 ggcgaaaaat ggccagcgtt tacgactaat ggacggctgg ctggtgacgc aggatcccca
     9121 gggcaattac tggtatctgc tgcacggcga actggcggga tcgtcgtttg atatgcagca
     9181 aacccaccag ctcatcacga ccctgaatac gctggaaaag gctctgaaaa cgcgttaccc
     9241 gcaggcgcag ttgctctcac gcggcacggt gttttacagc gattacgcca gccaacaggc
     9301 gaagcaggat atctccaccc tgggcgtggc aacgctgctg ggggtgatat tgctgattgt
     9361 ggcggtgttc cgctctttgc gcccgttgct gctttgcgtg atttccatcg gcatcggcgc
     9421 gctggcggga acggccgcca cattgttgat tttcggtgaa ttacacctga tgacgctggt
     9481 gatgagcatg agcgttatcg gcatttccgc tgactacacg ctctattatc tcaccgagcg
     9541 gatggtgcac ggcaacgacg tttcgccgtg gcaaagcctg gcgaaagtac gcaatgccct
     9601 gctgctggcg ctgctcacaa ccgtggcggc atatctgatt atgatgctcg cccccttccc
     9661 cggcattcgc cagatggcga tttttgccgc cgtcgggttg agcgcctctt gtctgaccgt
     9721 cctgttctgg catccgtggc tgtgccgtgg tctgccggtg cgcccggttc cggcgatggc
     9781 gctgatgcta cgctggctgg cagcgtggcg gcgcaataaa aaactgtcgc ggggtctgcc
     9841 cgttgcgctg gcgctgtttt cgctggcggg gatgtcaatg ctgcgcgtcg atgacgatat
     9901 ctcgcagtta caggcgctgc cgcagcacat tctggcgcag gagaaagcca ttaccgccct
     9961 gaccggacaa agcgtcgatc aaaaatggtt tgtagtttac ggcgactccc cacagcaaac
    10021 attgcggcga ctggagaaat ataccgcctc acttgagtat gcgaaaaaag aggggcttat
    10081 cagcaactac cgcactattc cgctgaactc cctggcgcgg caggaggaag acttagacct
    10141 gctgaaaacg gctgccccga cggtaacaaa agcactgcaa aatgcagggc tgacggcggt
    10201 gaaaccagat ctcaacgcca tgccggtgaa ggtcgatgaa tggctggcaa gccccgccag
    10261 tgaaggctgg cgtctgctgt ggctgacgct ggaaaacggc gaaagcggtg tactggtgcc
    10321 agttgaaggg gttaaaagta gcgcattgtt gcaggaaatc gccacatatt acccttgcgg
    10381 cattgcctgg gttgatcgca aaagcacctt tgatgaattg ttcgcacttt accgctacgt
    10441 cttaaccggc ttgttgctgg tggcgctggc agtgattgcc tgcggcgcag tggcccgtct
    10501 cggctggcgc aaagggctta tcagcctggt gccttcggtg ctttcgctgg gctgtggtct
    10561 ggcggtgctg gcgatgagcg ggcaggcggt gaatctcttt tcgctgctgg cgctggtgct
    10621 ggtgcttggc atcggtatca actacacgct gtttttcagt aatccgcgcg gtacaccgtt
    10681 aacttcgcta ctggcgatcg cgctggcaat gctcaccacc ttgctgacgc tgggaatgct
    10741 ggtattcagc gccacccagg ccatcagcag ttttggcatt gtgctggtga gcggtatttt
    10801 caccgccttc ctgctttcgc cgctggctat gcccgataaa aagagaacaa aaaaatgatc
    10861 aaatccacat tctggcgagc gttcgccctg accgctacgc ttatcctcac cggctgtagc
    10921 cactcgcaac cagaacagga aggccgcccg caggcgtggc tgcaacctgg cacgcgcatc
    10981 acgctgccgg caccagggat ttcacccgca gtcaattcgc agcaactgtt gaccggcagc
    11041 ttcaacggca aaacccagtc gctgttagtg atgcttaacg ccgacgatca gaaaatcacc
    11101 cttgccgggc tgtcgtcggt cggcattcgc ctgtttctgg tgacctacga tgcgcaaggg
    11161 ctacgcgccg agcaatccat cgtcgtccca cagttaccgc ccgcaagtca ggtactggct
    11221 gacgtgatgc tcagccactg gccgattagc gcctggcaac cgcaacttcc cgcaggatgg
    11281 acacttcgcg acaacggtga caaacgcgag ctgcgtaacg ccagcggcaa actggtcacg
    11341 gaaatcacct atctgaatcg ccagggaaaa cgcgtgccaa tcagcattga gcagcatgtc
    11401 tttaaatacc acatcaccat tcaatactta ggtgactgat atgatttata tttccgccgt
    11461 cggcatgatc aacgccctgg gaaataacct tgacgaaatc gcagccaacc tggctcgcgg
    11521 tgtcgccccc ggcatgcgcc cgcgcgcagg ctggttgcag gggcatccgc aggcggtcct
    11581 ggctggcgtg gatggcgagc ttccgcttat cccggaaaaa ttcgctgcgc atcgctcgcg
    11641 caacaatcag atcctgctgg cggcgctggc gcagctccag ccgcaggttg atgacgctat
    11701 cgcgaaatat ggctgcgagc gcatcgcgat tgtgcttggc accagcacct ccggcctgca
    11761 tgaaggcgac acgcacgtta acctgcacac ccacgggcag ccaagcacca cgtggcacta
    11821 tgcacaacaa gaactcggcg atccgtcgcg ttttctcagc cactggctgg cgctcgacgg
    11881 cccggcctat accctttcaa ccgcctgctc ttccagcgcc agagcgataa tcagcgggcg
    11941 caggcttatc gaagcggggc tggtcgatgc cgccattgtg ggtggcgcag acaccctgag
    12001 ccggatgccg attaacggtt ttcacagcct cgaatcgctg tcgccaacct tatgtcagcc
    12061 gtttggtcgt gaccgcgcag gtatcaccat tggtgaaggg gcggggctaa tgctgctgac
    12121 ccgggaaccg cagcccatcg cgttgctggg cgttggtgaa tcgagcgacg cttaccatat
    12181 ttcagccccg catccgcagg gcgaaggagc gatccgcgct attaatcagg cgctgaccga
    12241 tgcgcagctt acgccagatg acgtcggcta tatcaacctg cacggcaccg ccacacaact
    12301 caacgatcag attgaatcaa tggtggttaa cgccctgttt ggcgaacgcg taccgtgcag
    12361 ctccaccaaa catttgaccg gacacacgct gggcgcagcg ggcattaccg aggcggcaat
    12421 tagtatgttg attttacagc gtgatttacc gctgcccgct caggacttca gcctgtcgcc
    12481 gcgcgatccc acgctgccac cgtgtggcat tatcgaaaaa ccgcagccgc tggcacgtcc
    12541 ggtgatcctg tccaattcct tcgcctttgg cggcaataac gccagcattc tgctcgggag
    12601 ggtttcgtga gccactattt atcccccggc gcttatctgc cccacgacgc gcccatgtta
    12661 ctgctggaag acgtggtgag cgtcagcgat gacagcgcgg tgtgccgcgt gacggtttcg
    12721 cccagtggcg tactggcacc gtttctcgac ccggacggca atctccctgg ctggtttgcc
    12781 cttgaactga tggcgcaaac cgtcggcgtc tggtctggct ggcatcgcca ccagcagggc
    12841 aaaaacagta ttgagttagg gatgatactg ggcgcgcgtg aattgctttg cgccgctggc
    12901 attctgcccg caggccagac gctgaccatt accgtcaaac tgctgatgca ggacgatcgc
    12961 ttcggcagtt ttgagtgcag catcaacgac ggcgaggcga cgggccgcat taacaccttc
    13021 cagccgaccg cggaagaact taccaccctg tttcaacaag gagcgtcctg atgagtcgtt
    13081 cagttctggt tactggtgcc agcaaaggca ttggtcgcgc catcgcctgc caactggcgg
    13141 cagacggctt taacattggc gttcactatc accgtgacgc cacaggcgct caggaaacgc
    13201 tgaatgccat tgtcgccaac gggggtaacg ggcgtttgct cagtttcgac gtcgcaaacc
    13261 gcgaacagtg tcgggaagtg ctggagcatg agatcgcgca acacggtgcc tggtacggcg
    13321 tggtcagtaa cgccgggatc gcccgcgatg ccgcttttcc ggctttaagc gatgacgact
    13381 gggatgcggt gatccacacc aacctcgaca gtttctacaa cgtcattcag ccgtgcatta
    13441 tgccaatgat cggctcgcgc cagggtgggc gcatcatcac gctgtcctcg gtttccggcg
    13501 taatgggcaa tcgcggccag gtgaattaca gcgcagccaa agccggaatt atcggcgcaa
    13561 caaaagcgct ggctatcgaa ctcgcgaagc gcaaaatcac cgtcaactgc atcgcgccgg
    13621 ggcttattga taccgggatg atcgaaatgg aagaaagcgc tctgaaagag gcaatgtcga
    13681 tgatccccat gaaacgtatg ggccaggcgg aagaagttgc cgggcttgcc agttatttaa
    13741 tgtctgatat tgcgggttac gtcacccgcc aggttatttc catcaacgga gggatgttat
    13801 gacacgtcgc gtagtgatta caggaatggg cggcgtgact gcctttggtg aaaactggca
    13861 ggacgtttct gcacgcttac tggcgtatga aaatgcggtg cgcaaaatgc cggagtggca
    13921 ggtctacgat gggctgcaca ccctgcttgg cgcgcctgtt gatgatttca cgctaccgga
    13981 gcactacacc cgcaagcgta tccgtgcgat gggccgcgtg tcgcaaatgt cgacccgtgc
    14041 cagcgagctg gcgttagagc aggcagggtt gattggcgat ccgatattaa ccagcggcga
    14101 aacgggcatt gcgtacggtt catcgaccgg cagtaccggc ccggtaagcg agttcgccac
    14161 catgctgacg gaaaagcaca ccaataacat cactggcacc acctatgtgc agatgatgcc
    14221 gcacaccacc gccgttaata ctggcctctt tttcggcctg cgcgggcggg tgatcccaac
    14281 ctccagcgcc tgtacttccg gcagccaggc aatcggttat gcgtgggaag ccattcgcca
    14341 cggttatcaa acggtgatgg ttgctggtgg tgccgaagag ttatgtcctt cagaagcagc
    14401 ggtatttgat acgcttttcg ccaccagcca gcacaatgat gcgccgaaaa ccacaccttc
    14461 accgttcgat gaaaaccgcg atgggctggt gattggtgaa ggcgcaggta cgctcattct
    14521 cgaagaactg gagcacgcca aagcgcgcgg cgcgaccatt tacggtgaaa tcgtcggctt
    14581 tgcgacgaac tgcgacgcgg cacatattac ccagccgcag cgcgaaacca tgcagtattg
    14641 tatggagcaa tcgctgaaaa tcgccgggtt aagcgcacaa gatatcggct atatttcagc
    14701 gcatggtacg gcgaccgatc gcggtgatat ggcagaaagt ctggcgacag caaccattta
    14761 cggtgataat gtgccgctct cctcgctgaa aagttatttt ggtcatacgc ttggtgcctg
    14821 tggcgcgctc gaagcctgga tgagtctgca aatgatgcgc gaaggttggt ttgcgcctac
    14881 gctaaacctg aataaaccag acccgaattg tggcgcgctg gattacatca tgcatgaagc
    14941 ccgcaaggtt gattgtgagt ttttgcagag taacaacttt gcctttggcg gcattaatac
    15001 atccatcatt attaagcgct ggccctgagt tatacaacca catggacatc ccatgtggtg
    15061 attcttcaaa aaatctgaat caatgaatat gaacctcaaa ttctcttacg catactgaag
    15121 agcattccgt ataacgattc agcagacaaa actttatcag tccacacgaa tcaacggatg
    15181 cacagatgac acgcccggtt atcaccgggc aatggttgcc ataacgggta acataaggga
    15241 ctgaacagtt gtctttagcg caacgaactg atctaacgta cctgaacgga caacctgaaa
    15301 acagccttcc atcctgcatt gtccgccagt gcttattacc agccacgtct gttgcagtaa
    15361 attaatcagt tgagaacatt cttcatcaat cgtgacgctg gctcgcgcat ggcagaaaag
    15421 gtcctgactg agcaacaaca ggcatcgtac tgttgctgct ccatgttgtt tatatgaacg
    15481 catcccgttt gcaaggcttc cgtgtttttg acgttgacgg tagattgctg ctctatatcc
    15541 ttattgcagc cggtgctgcg ggcctcaatg agattcgctg actctcgcct tattcacgca
    15601 acgagcttta agctcagaaa aacatccaag tttggtgagt cacagtctta cctgtcgttg
    15661 tcatcaatat aggaatggat aatctggaga gcgatactgc aggctgatat tgcggttttg
    15721 cgacaagttt ttagcatccg cttctacgcc aaatacctga agtaggaatg gatgcgttag
    15781 cagcatacat ccggaactgc gcggggaata gccggaaacg gctatcccta ccacaatagc
    15841 taactttgta atagcgctct tttaggctca tgtactctga cgtaattcta tctggtagtt
    15901 gaattgcacg atacgcgact cttctgattc atcatgatta ttattcaata gtccaatcag
    15961 taacttacct gtctctatcc ccattttttt gtagtcaata cggatggatg tcaaagttgg
    16021 gtgtgtttga tcgcaattgt ctgaaccgtc aagacaagct acagcgatat ttccagggat
    16081 ttttaacaaa cgccgctggc actcaaacat tatacccagc gcaatctctt cgtggctgca
    16141 aatcacagca tccagctctg gttgtcgcag taatatctcg tttaatgcgt aacgccccaa
    16201 ttgcagactt gcggcatccg gcatagtgat aacaagatca gtgttctggt aatggctcag
    16261 cattgcttta tgccagccgt tcagttgctg acgttgcagg cggttgtcca tatgcgcacc
    16321 gataaatccg atattcttat aaccctgctc cagcagatgt tcggtcagtt tatgtgatgc
    16381 ttcaaaaaat gctgtttcga tggtaatttg agccggactg aaaggggaac cgataacgtt
    16441 aatggtaggt atatttgttt tctcgagtat ctggtgagtt ttatcagcca actgagaacc
    16501 aaaaataact aacgccgccg ggttgccttg aagaagcgtc atcagaattt cagcttcttt
    16561 gagctgatta tactcatgac acccaagcag tagctggaat tcatttttat taagcacctg
    16621 ctgtaatgac tgcataaagc gggagctggc tttatctgtc agggaaggaa ccagtacggc
    16681 gactgtatga ctgtgaccag atgcaagggc gccagcagtt ctgttgggaa tataacccaa
    16741 ctcttctacg gcctgttcta tcttttccct cagcttgtca gaaacctgtt ccggtgttct
    16801 cagcgcgcgg gaaacggtca tggttccgac acctgcaaag ttagcgacct cctgcagcgt
    16861 cacccgtccg gtaccacggc gcttacgtgt tttttccatt tcgactccat ggataattac
    16921 aaccagctgt tttcattact tttatttcac cattactctt tccagttttt gcccacggta
    16981 gcaaatatcc tgttgcattg tatatgcagg aaccgaatat agccaaagca tggcggcaga
    17041 aaaatgatag cgctatcaca aagattggta gcgttatcag atagcgctat caaatgcgac
    17101 aatcatcaca ttaattaatg cgtaaaatat ctactctcct gttaattatc atcgggagtt
    17161 atcagtagca tgttaaaaaa tctattaaat acagaggtcg ttcaggtcgt ggaacaggtc
    17221 aaagactggc gggaagccgt ggcgatttca tgccgaccgt tgattgaaaa cggctctatc
    17281 gaaccgcgtt atgttgatgc gatctatcac tcgcacgaca ctatcggtcc gtactatgtg
    17341 gttggccctg gtattgcgat gcctcatgcg cgcccggaag agggggctaa caagctgtca
    17401 ttagcactga ccctgatccc ttcaggtgtg aattttgatg ccgatgaaaa cgatccagtg
    17461 aaattactga ttgtgctggc tgcaaccgac agtaccagcc acattgaagc gatttctcaa
    17521 ctcgccaagt tattcgataa cgaaaaagac atacaggcca ttttgacggc caaaacgacg
    17581 caggacattt tgtctgttat tgcgcgctat taatccagag gaacatttat gaaaattaca
    17641 gtggtttgcg gtaacggctt aggcaccagc ctgatgatgg aaatgagcat taaaagcatt
    17701 ttgaaggatt tgtccgtcag cgctgatgtt gatcacgtcg acctgggctc cgcgaaaggg
    17761 acgccgagtg atatctatgt cggcaccaaa gacattgccg agcagttagc cgcgcagtct
    17821 gtcgcaggca aaatcgtcgc actggaaaat atgattgata agaaagccat gagagaacgt
    17881 ttgtccgtgg tattaaccga attaggcgca ctgtaagctg ggggcgatat ggatttcttt
    17941 cgttttttga tgagcgatgt actttccgaa cccgcagtgc tggtcggttt aatcgccctg
    18001 attggcctga ttgcacagaa aaaaccggta accgaatgta ttaaaggtac cgtcaagacc
    18061 attatggggt ttgtgatttt aggcgcaggg gcgggtttag ttgtgtcgtc actgggtgac
    18121 ttcgcaaaca ttttccagca tgcctttggt attcagggtg tagtgccgaa caatgaagcc
    18181 attgtttccg tagcgcagaa aagcttcggg aaagaaatgg cgatgattat gttctttgcg
    18241 atggttatca acattatgat tgcccgtttc acaccgtgga aatttatctt cctgaccggt
    18301 catcacacgt tgtttatgtc gatgatggta gcggtaattc tggcaacagc aggcatgacc
    18361 ggcattacgc ttatagccgt tggctctttg gttgttggcg tggcaatggt ctttttcccg
    18421 gctattgcgc atccgtacat gaagaaagtg accggttctg atgacgttgc catcggccac
    18481 ttctcaacat tatcttatgt attggccggt ttcatcggca gcaagtttgg taataaagag
    18541 cactcgactg aagacatgaa tgtgccgaaa agtctgctgt tcttgcgcga cacgccggtt
    18601 gctatctctt ttaccatgag cataattttc ctggtgacgt gcctgtttgc gggtgcagat
    18661 gcggtgaaag agcttagcgg tggtaaaaac tggttcatgt tctctatcat gcaatccatt
    18721 accttcgcag ctggcgtgta catcattctg cagggtgtgc gcatggtgat tgcggagatt
    18781 gtcccggcat tcaaaggtat ttcagacaag ctggttccga atgccagacc tgctctcgat
    18841 tgcccggtcg tcttccctta cgcacctaac gctgtactgg ttggtttcct gagtagcttt
    18901 gctgcaggtt taatcggtat gttcacgttg tatctgctga acatgatcgt gattattcct
    18961 ggtgtggtgc cgcacttctt cgtaggtgca gctgcagggg tattcggtaa tgcaacgggt
    19021 ggacgtcgtg gtgcgatttt gggtgccttc gctcagggct tgcttattac cttcctgcca
    19081 gtattcttat tgccggtgct gggtgatatt ggttttgcta acaccacctt cagtgatgct
    19141 gactttggcg cactgggtat tttgttaggg attatcgttc gctaatattt tgatgacatt
    19201 aatatcctcc aggatttcag gaacgtggag gatattaatt cctcttattt tctccatttt
    19261 tctttcttcc agtcaggact gcatccgatt aatcgtaagc gctccgagaa gtacgtagcg
    19321 taaggattat tttacagctg agaagtttca gtgcagcagt tcatgcacac ggttcgacgg
    19381 ctagtcattg atcttcataa ttacctcgcg taaaaaacgg ggtaaggccg ctcatccagc
    19441 gtcatctcca caggtaataa ttcaacgcca atgccagaag agttcgttac cggaagacgc
    19501 cgtgatatac gccctttgat cagcgagagc ctgagccatt tgaacagaag attatcattg
    19561 atatcatgct cccgggcaat acgggcaaca gaaccccttg gttgtaaagc cagtttaacc
    19621 atttgaagtt taaactcatt tgaaaatgac ctgcggggtt caactgataa tgttttttgt
    19681 tccataacag gtgtccacta gttgaaaagg tgggtaccta tgttaccaat actggcttaa
    19741 tggctacata cagcagtcgg tttacgcata cacttttata gtgcactccg gcctcatgaa
    19801 tataacggtg gattaccgcc gaacgaatcg gaaaatagat actggaaaaa aactgaaatt
    19861 gtgaccagtt tttgttgacc actacaaata aataaaagag ttgcagtgct tgctcggtaa
    19921 gaaaacaatg gaggttaagc tcctcaaaga agctgtggag tacaccactc tacagtagtt
    19981 gcgcaaattt ggagtggacg ataatgtgat cgtctataag ggcaacgcta tcatagtctt
    20041 gtcctggcgg gtaaaaaaac gcgcttacct taacgataag cgcgccgctg ttcaggcctt
    20101 gagtggttat tcaattcctg tggtgactgt aaaagtgcgc gtttgctgcg gtgcaacctg
    20161 aatcagcgtg ccattacgtt gcgcggcaag atacccctca ggccgacagg ttgcaggtaa
    20221 tgcaaaggcg gctacctgtt gctctccgtt ataaaggatc cagcgtgtca cataatttag
    20281 ttcagcactg tagaaacgag taacaaacgt agtgccatcg ggagagatca tgcgaaactc
    20341 tggctgctct gtataagcgt ccagtttgtc tgcaaagaag acaatttctg gatcataaaa
    20401 ttccggttga ctcagcgtcg acagagaggc ttctccctgc ataatccgtt gattaaacgc
    20461 cagccactga gcggtgggat taacatgcga aggcactgat tcacgcaatc ttaatatttc
    20521 gtccgggata ttctggctga atgtagcatt tggtatatat gcataattca tgtggcacat
    20581 atattgtagt ggcatatcta cagaagccag attggttacg gccatcttaa tatcgaacag
    20641 tgtagaggat ttgtgaagga ccactgttgg ctgagccaga taatgatgac cgaaacccat
    20701 tacatactcg taacgcccgt taaggcgtaa catatctccg tctaattcca gccatgcttc
    20761 atccatcgcg gcacaggcca tttcaccgtg tagcagatga gtatcttccg cagatgggca
    20821 gccattagcc agcaaacctg aatgaaaagc aaaacagcca taggtctcta tcacctctgt
    20881 cgccggttta ggctggcgaa acatattgca catggtgagg ccgtgtccat caaattgcgc
    20941 atcccaaatc atctgcccca tccagggaag aataatcaaa tgtccacgac tgtttgcaat
    21001 tttaagcccc tcgacaccgc tgtcatatcg aaaagacgtg acagtaaaat cactattttc
    21061 cagcaagata cgaggtttct cgccaaaaag cgcccgccac aaattaatac gcgtactcat
    21121 aacggttctc ctcaggacgc tgtgacttca gccagtgcgg tacgtacttt gctttcacgc
    21181 cagaagtaga ctccgacata gacaaagcag agcatagaaa ccaggaatga aagctgtagt
    21241 gagtggaaca tatctgcaat atatccctga attgccggaa ccaccgcggc accgacaata
    21301 gccataacaa tgactgctcc tgccatttct gtatgttcgt tatcaactgt atccagtgtt
    21361 cctgcataga tcgtcgccca gcaagggcca aacaaaacac ttaccaggac ggcgacatag
    21421 accgcgctga aacttggagc cagtgcaaca tatgccagga acagcgcccc tataacggaa
    21481 tagagaatca atactttttc cggattaaaa cgcgtcataa ggatattggc tataaacttg
    21541 ccaataaaga agcaggcaaa gctatagacc atgaagtttg aagcatcacg ttcgttgata
    21601 tcgcccaact ccagcgccag acggatggta aatgaccata ctgcgacctg catacccaca
    21661 taaaggaact gcgccacaat accgcgacga aagcgcggat ttctagccag atagcgcagc
    21721 gtatccattg ctgacgggcg tttatggtga cttgtctgtg ccactttaca ggttgggaag
    21781 cgggttaaaa ggaacaacac catgaccaca actagaatca taatcatata cttatacggt
    21841 tcaagggtgt tctctaacat cagcacctta aagttgtgaa tttgctcggc gttcattcct
    21901 gacatctgct tttcaaggct ttccccctcg gagaaaacca gatatttgcc caataaaata
    21961 ccagacgcag caccaatcgg ataaaaggtc tggctgatat tgagccgcaa tgtggcatag
    22021 gcttttggac cgatcattga actgtatgtg ttcgctgcag tttcaaggaa actcaggcca
    22081 atcgcaatcg caaaaatagc tgcaaggaac atagtgtagg ttgccatatg cgaggcaggg
    22141 aaaaaaagtg tacaaccacc aatatacagc gtcaggccaa ttaaaattgc caccttataa
    22201 ctggtctttt taatcacaag ggatgctggt attgcaatta aaaaataacc tccataaaat
    22261 gcgctctgca ccaatgctga agcaaagtta cttagcgaaa atacactttt gaattgagtg
    22321 attaatatgt catttaatgc agctgcgcat ccccatagcg ggaataaaca cgataacaaa
    22381 ataaactgga acaagggagt cttattcaga tacccatcag gcatctgaat gatgttttta
    22441 tcgttcatag tgctaccttt aactgtgcag gatgattatt cgtttaaggt taaaaattca
    22501 ttaaattgtt caatactcgg ataagatgat tgcgtacctt tccctgtgac gctgaaagcg
    22561 gcaaagagag cggctttttt caaagcggct tcaacatcac cgctttgaac ataataatgg
    22621 gaaaagcaac caataaatgc gtcaccagcg ccactagtat caacagcatt tactttgaat
    22681 gcaggaacat ggacttcctg atcgcgggtc atccataatg cgcctttttc gctcatggta
    22741 acaataatat tgttcagccc tttatcaact aacgaacgtg cggccaaacg aatatgatca
    22801 taagtatcaa ccgacatacc ggttaatatt tccagttctg tttcattcgg gataaagaaa
    22861 tcacatttgc aggcataaga catatctaac tcacgcaatg ccggagccgg atttaataac
    22921 acttcaatac catttttctt accaaactca atcgcgtggt aaactgtttc cagttgaact
    22981 tccagttgta aaacgatcaa tttgcatttt ttcagatctt ctgcagctcg atcgatatct
    23041 tccggggaaa gaaatttatt cgctccctta attattaata tactattgct cgagttggca
    23101 ttaacaaaga tcggtgcaac accactgctg gtacagggga ctttctcaac ataagtggta
    23161 ttaattcccc atgattcgag attacgaata gtattatccg caaaaatatc atcacctact
    23221 ttagtcagca tcaggacttt tgaattcaat ttagccgccg ccaccgcttg attagcacct
    23281 ttcccaccac atccgatttt gaaggcaggt gcttccagag tttctccttc tttaggcatc
    23341 tgattagtgt aagtaatgag atccaccata ttggaaccaa taactgcaat gtccatttca
    23401 ctacctctta taaactttcg cataacaatg gtatttaaat aacattagca tgttactttt
    23461 acatcatttg tgactgagat cgcgattagc acatcaaccc gatgtttatt taatagactt
    23521 ccagtcttat cactcaggcc aacactatct aatcataagc aacctaacag gattaatacc
    23581 gaaaattcag cagtctatac ccttttcatt tcaaagggtc ggtcgtatag tatggtaact
    23641 aaaacaatgt ttactaatgc cataatgtta tttttataac attttacgga gagagttgat
    23701 ggaaacgaag caaaaagagc gtatccgacg tttgattgaa atacttaaga aaaccgacag
    23761 aatccatttg aaagacgcgg cacgaatgct ggaagtttct gtaatgacta ttcgtcgcga
    23821 tctccatcag gaagatgaac ctctgccact gaccctactg ggtggctata ttgtaatggt
    23881 gcataaaccc gcaccatcca tgccagtaat ccaggacgtt ccgagaaatc atcgtgatga
    23941 cttacctatt gcaattctgg ccgccggaat ggttaatgaa aatgatctga tcttctttga
    24001 taatggccag gagataccgc tcgttataag catgatcccg gatgcaatca ccttcactgg
    24061 catctgttac tcacatcgtg tctttgttgc gttgaatgaa aaacctaatg tgacagcaat
    24121 actttgtggt ggtacgtatc gtgccagaag tgatgctttt tacgatgcca gtaactcttc
    24181 gccattagac tctctcaatc cgcgaaaaat atttatttcc gccagcggtg tacatgatca
    24241 ctttggcgtc agctggttta atcccgaaga tcttgccact aagcgtaaag cgatggcccg
    24301 tggactaagg aaaattttgc tcgcccgcca cgccttgttc gatgaagtag cctctgccag
    24361 cctcgcaccg ctctctgcat ttgatgttct gattagcgag cgtccgttac cggcagatta
    24421 tgttacgcac tgccggaatg cttctgtaaa gatcattaca cctgattcag aagacgaatg
    24481 acttactgaa aaaacaccgt actcttgtta aacatcgtcg gattggactg attacgttgc
    24541 actttcatca catattccag tttatcaatt tggcttatca tctactccag acgctggtca
    24601 tcattgacca atagtctgat atggcttttg tcgctgtcct gaatcggcag gcagaaaatg
    24661 ccttcaacgt taaaagcacg acgggaaaaa gcccacaaag gttagtcatt acgcccggat
    24721 aataaacagg ctcctccctg ttatggaagc cgttgtattg tccataatgt ctgggttttt
    24781 aatgcacact ggataacaac cggacgaatc ttcaaatatt actggcgctg acagccggtt
    24841 tgaattcatc tcacaaccct gcatagagcg aatctcctgc ctgcacgtca ctccactcca
    24901 tggtatcaac ttcacactct ttatctgcgg ctagtttcag tcaccagata agcatcgact
    24961 atgaaaggtg agccatgaca acataacgtt ggtaacgctc tgacgcctta acggaagatg
    25021 cctgccacca cagggaatgt aaacgactga agtgtggcct ttaatgccgt gaacggctca
    25081 tggtctcctg gcacggttgc cgccccaacc tgtaacaaca ttccacagta caatgtctgt
    25141 cagagccgga gcctcccatg cctgttgaat aaaaccccgg atgcttcccc cgattctgca
    25201 agtaatactt cggttattta cactatttta tccctgttga atttggttaa gccggtgcag
    25261 acgcgccggg aaacgcacgc ctgaaccggc acattaaaat acaatacaac cccctgttac
    25321 tggagatacg aatggaatga cgaggcagcc tgtcagtcac aggagggctg ttggctcaaa
    25381 atcaacggaa aggccaccaa caccggaatt aagctccaca gtccccgcac tggcaatcca
    25441 ttcccgggaa cggcatcacc gcaaactgcg ccagaggcaa ccattaca
//
LOCUS       DAPHDX010000063        25390 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPHDX010000063 DAPHDX010000000
VERSION     DAPHDX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.063"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            198..617
                     /locus_tag="RH058_002509"
     CDS             198..617
                     /locus_tag="RH058_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV3138084.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            617..1885
                     /gene="umuC"
                     /locus_tag="RH058_002510"
     CDS             617..1885
                     /gene="umuC"
                     /locus_tag="RH058_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV3138085.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1931..2461)
                     /gene="dsbB"
                     /locus_tag="RH058_002511"
     CDS             complement(1931..2461)
                     /gene="dsbB"
                     /locus_tag="RH058_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV3138086.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2607..4148)
                     /gene="nhaB"
                     /locus_tag="RH058_002512"
     CDS             complement(2607..4148)
                     /gene="nhaB"
                     /locus_tag="RH058_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV3138087.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4370..5089
                     /gene="fadR"
                     /locus_tag="RH058_002513"
     CDS             4370..5089
                     /gene="fadR"
                     /locus_tag="RH058_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV3138088.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5141..6673)
                     /locus_tag="RH058_002514"
     CDS             complement(5141..6673)
                     /locus_tag="RH058_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV3138089.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7003..8301
                     /gene="dadA"
                     /locus_tag="RH058_002515"
     CDS             7003..8301
                     /gene="dadA"
                     /locus_tag="RH058_002515"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV3138090.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8311..9381
                     /gene="dadX"
                     /locus_tag="RH058_002516"
     CDS             8311..9381
                     /gene="dadX"
                     /locus_tag="RH058_002516"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV3138091.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9767..11503)
                     /locus_tag="RH058_002517"
     CDS             complement(9767..11503)
                     /locus_tag="RH058_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV3138092.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11598..12512)
                     /gene="ldcA"
                     /locus_tag="RH058_002518"
     CDS             complement(11598..12512)
                     /gene="ldcA"
                     /locus_tag="RH058_002518"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV3138093.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12612..13223
                     /gene="emtA"
                     /locus_tag="RH058_002519"
     CDS             12612..13223
                     /gene="emtA"
                     /locus_tag="RH058_002519"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV3138094.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13225..13959)
                     /locus_tag="RH058_002520"
     CDS             complement(13225..13959)
                     /locus_tag="RH058_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV3138095.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14160..14414
                     /locus_tag="RH058_002521"
     CDS             14160..14414
                     /locus_tag="RH058_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV3138096.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14592..15032
                     /locus_tag="RH058_002522"
     CDS             14592..15032
                     /locus_tag="RH058_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138097.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15111..16808)
                     /gene="treA"
                     /locus_tag="RH058_002523"
     CDS             complement(15111..16808)
                     /gene="treA"
                     /locus_tag="RH058_002523"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV3138098.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17128..18546)
                     /gene="dhaM"
                     /locus_tag="RH058_002524"
     CDS             complement(17128..18546)
                     /gene="dhaM"
                     /locus_tag="RH058_002524"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV3138099.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVVPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18557..19189)
                     /gene="dhaL"
                     /locus_tag="RH058_002525"
     CDS             complement(18557..19189)
                     /gene="dhaL"
                     /locus_tag="RH058_002525"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV3138100.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19200..20270)
                     /gene="dhaK"
                     /locus_tag="RH058_002526"
     CDS             complement(19200..20270)
                     /gene="dhaK"
                     /locus_tag="RH058_002526"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV3138101.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20498..22417
                     /gene="dhaR"
                     /locus_tag="RH058_002527"
     CDS             20498..22417
                     /gene="dhaR"
                     /locus_tag="RH058_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV3138102.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22517..25384)
                     /locus_tag="RH058_002528"
     CDS             complement(22517..25384)
                     /locus_tag="RH058_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3138103.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYSKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPTPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         6271 a         6325 c         6555 g         6239 t
ORIGIN      
        1 tctatttttt agccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
       61 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
      121 tagcctgaat cagtattgat ctgctggcaa gaacagacta ctgtatataa aaacagtata
      181 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
      241 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgttg
      301 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
      361 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
      421 atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
      481 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
      541 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
      601 ttaaggcgat gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga
      661 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg
      721 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg
      781 gttcaaacaa aaagatctgt ttcgtcgctg tggcgtggtt tgctttagca gcaattatga
      841 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
      901 cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga
      961 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     1021 tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg cggcaaaaaa
     1081 atggcagcgg cagacggggg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt
     1141 aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca gcaaaaaact
     1201 ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc ggtttatccg
     1261 taaacatttt aatgtcgtgc tcgaaagaac ggtgcgtgaa ctgcgcggcg aaccctgttt
     1321 gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct cgtttggtga
     1381 acgcatcacg gattatccat cgatgcggca ggccatttgt agttacgctg cccgggcggc
     1441 ggaaaaactt cgcagcgagc atcaatattg tcggtttatc tccacattta ttaagacgtc
     1501 accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac tgctgacgcc
     1561 cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg ccatctggca
     1621 agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt
     1681 cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc aattgatgac
     1741 ggtaatggat acactgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg
     1801 gatccagcaa caatggcaga tgaagcgtgc catgctttca ccacgttata caacgcgaag
     1861 ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc ccgcaggagc
     1921 gctgaaggga ttagcgaccg aacaggtcac gttttttcgc tttaaacggc tgggaaatca
     1981 ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac tgcggcattt
     2041 ccagacctaa aaaatcccac tgacgctcgg cacaatcgcc agaggcgaca aacacttgcg
     2101 gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca caggtggcaa
     2161 acggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca ccgcggaacg
     2221 cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg gcgatcgcgc
     2281 caatcagcgc agcacccaga acgccgaata acgcacaacg ttcataaata cagagcacgc
     2341 aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt gccagagcag
     2401 taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa aatcgcaaca
     2461 taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat tcgctgcgcc
     2521 accaggaggc agcaaaaata ggaagcaatt ggacaattat cgggcgaaaa ggcaaaccgg
     2581 gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat ttgcataaac
     2641 cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag tgtcagtacg
     2701 agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat caatggcgcg
     2761 agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc gacggagggc
     2821 agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa cgtgatagcg
     2881 ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa gacgttatcc
     2941 gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc atgttccgat
     3001 gcctgcaaca caaactgaat aattggcgaa aacagttgtt ggtcgataat caccgcgact
     3061 accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa ggctttaccg
     3121 atagcatgtt catcggtgac accggtcaat gatgtcgcca gaataatgac tgacaaacca
     3181 atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac gccaataatc
     3241 gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct ttgatcgtca
     3301 aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc aaaccaacgc
     3361 agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac ggtcaccggc
     3421 gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat gatcaggttc
     3481 tgtggttcgc ccaccatggt catcacgccg cctaatgcag tgccgacacc ggcatgcatc
     3541 atcaggctac gcagaaagcc acggaactgt tccagaacca ctttgtaatg cttgtcgata
     3601 tgactatcgt cttgcaggtc ggtatcttcg gtacgggaag aggctacgcg gtgataaata
     3661 ccataaaaac cgacagcaac gctgatcacc accgccacga cggttaaggc atcgaggaac
     3721 gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg
     3781 cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata gatacccgcc
     3841 accataaaca tcaataacag caagacttca agatttgccg ccacctcttc acggacgtgt
     3901 tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc ggggagcagc
     3961 gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag caaccagccc
     4021 gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat gatgagggcg
     4081 agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg gccccaggag
     4141 atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat gtttaaacgc
     4201 aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga aagagatgcg
     4261 gaaaatgaag ccttgatccc ttttttcttc tttttgtctg ctatcagcgt agttagccct
     4321 ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta tggtcattaa
     4381 ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct ggaataaccg
     4441 cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa ttggcgtaac
     4501 gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt tgaccattca
     4561 acatggcaag ccgacgaagg tgaataattt ctgggaaacc tctggtttaa atatccttga
     4621 aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt tgctgtcggt
     4681 gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc ccgataaagc
     4741 gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct ttgccgagct
     4801 ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt acggtctgat
     4861 ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg ccaatccgga
     4921 agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca gtgaaggcgc
     4981 gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg agatttggca
     5041 ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat cccttccgtt
     5101 taaagagcaa acccctcaaa cgaggggttt tttgttgttt ttacagattt cccattcttg
     5161 gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg agcatcacat
     5221 caaatcccca caggcgatgc acatgcttca gcacttcttt gcgcccccga tccagcggtg
     5281 cgcgattatg tggaatgtaa cgcagcgtca gcgaacggtc gccgcgcaaa tccacgttcc
     5341 agatctgaat attcggctcc agattactta agttatattg cgacgataac cggttacgga
     5401 tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta tgccgatcat
     5461 catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga aactggctga
     5521 taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc cagtcggaac
     5581 cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa atccgtttaa
     5641 tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac cacgggctgt
     5701 tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc ataaaacgtt
     5761 ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag aaggtcgccc
     5821 agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc accttacgca
     5881 caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc tccataaaat
     5941 agagcaggtt ttcttgtggt tcggacggat agcggcgcgc ttcagcaacc gttttctctt
     6001 cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga tactcttcgc
     6061 gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt ttgtaccggt
     6121 ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact tcatcaacgc
     6181 cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa tcgacaatcg
     6241 aactggcgtc ggtccaactg cggaataagt aattgttttt aaagaaagag ttatgcccat
     6301 agcaggcgtg tgccatcacc agcgcctgca tggtaatggt gttctcttcc atcaggtaag
     6361 cgatacacgg gttagagtta atgacgattt cataggccag tccttgctga ccgtgcttat
     6421 acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag ttaattggca
     6481 tgccgacgct ggagtaggca tccatcatct gttctgaggt tatcacttca atctggtgcg
     6541 ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga taaacatcca
     6601 gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta ttcatagaat
     6661 cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga gcacctcatt
     6721 tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct ttgcgatttc
     6781 ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga taaaaagggc
     6841 gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat gtgagccagc
     6901 tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa gatgtaatta
     6961 gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt tgtcatactg
     7021 ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg acatgaggtc
     7081 accgtcattg atcgggagcc gggggccgcc ctggaaacca gcgccgcgaa tgccgggcaa
     7141 atctcccccg gatatgctgc accgtgggcg gcaccaggtg tgcctttaaa agcgattaaa
     7201 tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca gttccagttg
     7261 aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga aaacaaaggg
     7321 cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg cgccgaaacc
     7381 aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac cgaacaacag
     7441 tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc gtatcagctg
     7501 ctggaatcca gccgcctggc ggaagtggag cccgcgctgg cagaagtagc gcacaaactg
     7561 acgggtggtc tgcaactgcc caatgatgaa accggagact gccagctgtt tacccagaat
     7621 ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc cgttgaccaa
     7681 ctgctttgcg acggcgagca aatctacggc gtgaagtgtg gcgatgaagt gattaaggcc
     7741 gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg cattgttgat
     7801 attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca ggaagatggt
     7861 gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg tttcgataac
     7921 cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct gttgcaaccg
     7981 cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg tcatgtcgag
     8041 caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc ggttgtcggg
     8101 cgtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg ctggacgatg
     8161 gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc agcgatccca
     8221 tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg tccgggccat
     8281 ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc cagcctcgat
     8341 ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca cgcgcgcgtc
     8401 tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg gagcgcgctc
     8461 ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt acgtgagcgc
     8521 ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga tctggagatt
     8581 tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa agcactgcaa
     8641 aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg gatgaatcgg
     8701 ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc aatggcgaat
     8761 gttggcgaga tgaccctgat gtcgcatttt gccgaagcgg aacatcctga tggaatttcc
     8821 agcgcgatgg cgcgtattga gcaggcggcg gaagggctgg agtgtcggcg ttcgttgtcc
     8881 aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg gcctggcatt
     8941 attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac cggattacgt
     9001 ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc gggtgagcgt
     9061 gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat tgtcgccgca
     9121 gggtacgccg acggttatcc gcgccacgcg cctaccggta cccctgtttt agtggacggc
     9181 gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tagcggtcga tttaacgcct
     9241 tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat caaaattgat
     9301 gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct ggcgttacgc
     9361 gtcccggttg tgacagtgta acttgttgta agccggatcg gagacagcgt cttctggttg
     9421 cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac agagccattg
     9481 cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga actcaccgca
     9541 caggcgttct acataaaacg cttacgcttc attgttgact cgaactcgac ttcagataaa
     9601 tcacgcttta cccttgatgg agcctgtaca tagatttgtg taattgcctg attttgatat
     9661 gttcaattca acatcaaatg aaggttaaat tatggacgac aaacaattgc aggctcaggc
     9721 tgcgttcagc aaagcatcgc aaccggcgat agatgcttca ttaaatttaa gattcagctt
     9781 cctcttcagc cacccgtacg ccaatcttca acacttcatt atcttctttc tcggccaccg
     9841 tccagatcat cccggcaaac tctacttggt caccgacaac cggtgccgcg cctaacaact
     9901 gctggacaat ttcacccagc gtttgctgct tatcacgata ttctcgcccg tcttccaggc
     9961 catatatcag cgcaacatca gcatatttgg cgctggcttc gagaatgaag tcaccaaaga
    10021 agcgttgatc cagcgcgacc ggcggcgact ggctgaacag tttaccgagc gccggaagat
    10081 cgcgttcccg accaattaca cacaacacat cgccttcgcg cagtcgggtg ctgccggtag
    10141 gatgaagcaa ctggttatca cgaaacagtg ccgcaatacg cgtctctttt ggcatatgca
    10201 aatcgcgcag tgccgcgccc acgcaccatt tatcggcact caattgataa acaaactgct
    10261 cccacggatt ttccggatga atatccaggc caacgcgtga caccggacgt cccactggcg
    10321 gaaccaccac tttggctttt ttagccgccc acgagagtga tgttccctgc aagagcagtg
    10381 aaaccagaac cacaaagaag gcgacattaa agaacagacg tgcattctcc agccccgcca
    10441 tcatcgggaa cacggccagg atgattggca ccgcgccgcg taatcctacc cagctgataa
    10501 acacgcgctc acgcagattg aaaccgcgga aggggagcaa tccagcaaat accgaaaggg
    10561 gacgggcgaa gaatatcatc catgccgaca aaatgagcgc cggaatggca ataggcagca
    10621 gatcgcttgg gttaaccaat agccccagca ccaggaacat ggcgatttgc gccagccagg
    10681 cgaggccgtc gaaattttgc aggatgccgt agcggttgcg aatcggccga ttacccagca
    10741 gaaaaccgca cagataaaca gccagaatac cgctgccttc cagcgccgtg gttaaagcaa
    10801 aaattaaaat accgccgctt aatgccagca atggatataa tccggcaggc agggcgatgc
    10861 gattaatcat ttgcagcagt aaataaccac cgccaagtcc gatgacaatc ccgaggccaa
    10921 attgctgcag aatatcgacc acgaacatcc aactgacact gctttcatgt tgctggatca
    10981 tcgcaatcag ggtaatcgtc agaaagaccg ccattggatc attactgccg gattcaattt
    11041 ccagcgtcga gccaacacgt tcgttaagcc ccttaccacc cagcagagaa aagaccgctg
    11101 cagcatcggt agagccgacg atagcgccga ttaataagcc ttcaatcaaa tcaagattaa
    11161 acagccacgc cgccatcatg ccggttaaac cagaggtgat aagcacgccc agcgtcgcca
    11221 gcgacagtgc cggtcctaac gccacacgaa aggaactggc ctgagtgcgc atcccgccgt
    11281 cgagcaaaat aatcgccagt gccaggttac ttaccatgta ggcgaagggg taattatcaa
    11341 acgggatacc gccgacgcca tcgactcctg ccagcatgcc gatcgccaga aaaataacca
    11401 gaataggaat gccaagacgg gaagaaaatg aactaagtaa tatactgctg gtgacaagga
    11461 tggatcctaa gatgaaaagg ctaattattg ttgtggcatc caacggtcgg ttactcctga
    11521 ttacgctgtc tcttatataa accctaccat attagcggca gagacagcgt tttacttagc
    11581 cctgaagcgt gattttttta cattttaaga acaggatgac cgctaatcgt caactgagtg
    11641 ccttcccggg tgttattcag aatggcatgt gcgcccaacg gcagcgtaac cgtgcgttgt
    11701 tcatgaccaa aatcgaggcc ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg
    11761 tacactgact ccaggttgta acctgcgtca taatcattgg gcgtgctgcc gctaaagcta
    11821 ccgagaataa tcgccttctg acgtggcaaa attcccgcat gatagagctg caacagcata
    11881 cgttcgacgc ggaaagggtg ctcgttaata tcttccagta ccaggatacc gttctcaatt
    11941 tttggcatcc acggtgtacc aatcagtgaa atcagcatcg caagattgcc tccccacaac
    12001 gtgccttcgg tctgacatgt cgggccttcc ccttgccatt caatggtgaa ggttttattg
    12061 cgtaacgcca gccagaaatg gtgctcggta aaggcgttca gttcatccgc accaaagttt
    12121 gctaccagca tggggccgct aaaggtgatg acattgcctt gcgccagaag accgcactga
    12181 atggcggtaa aatcgctatg tccgcaaatg agtaacgggt catgttgttg gcgagccacc
    12241 agtgcctgcc agtcaatatc tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc
    12301 agcacgatgg tgttgggggt agtcagtctg gcaagggaat tgagatcttc caggcgctcc
    12361 gtttccgtac ccgcaaaacg ctcacaacga cgggcaataa cctcgacgtt attgacctga
    12421 tgcccctcgt cggtaaggcg atggatacca cgcagcgcgg cgtgctgttt aatgcagtaa
    12481 cccgatgggg caattaagtg aaacagagac atggcaattc cttgctgaca acagaaacga
    12541 aatgtatatc atgccgctta ggtgtgccgt tgtcacctca acggcgattc caggctataa
    12601 ggatagaaga agtgaaattg agatggtttg cctttttgat tgtgttatta gcgggttgtt
    12661 catcaaagca tgactatacg aacccgccgt ggaacgcgaa agttccggtg caacgtgcga
    12721 tgcagtggat gccaataagc cagaaagccg gtgcagcctg gggcgtcgat ccacaattga
    12781 tcacggcgat tatcgctatc gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg
    12841 ccattggttt gatgcagtta aaagcttcaa cctccggacg tgatgtttat cgccgtatgg
    12901 gctggagtgg tgagccgacg accagcgagc tgaaaaatcc agagcgtaat atttcaatgg
    12961 gggcggctta cctgaatatt ctggaaaccg gcccgctggc aggcattgaa gatccgaagg
    13021 tactgcaata tgcgctggtg gtgtcatacg ctaacggggc aggtgcgctg ctacggactt
    13081 tctcgtcaga tcggaaaaag gcgatcagca aaatcaacga tttggatgct gacgagttcc
    13141 tcgatcacgt agcgcgaaat catcctgcgc cgcaggctcc gcgctatatc tacaaacttg
    13201 agcaggcact ggacgcgatg taaatcagtc gcgcactttg tccgcttttt cccgggcttc
    13261 tcgctcgaga gagaaaataa tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag
    13321 aaaacggaag ctcagacggg gagtggtgat ggtttcattc ttgccatcaa tcactttgcg
    13381 ctcgctgatg gagattaact gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac
    13441 ttcaatctga gcgaagcgca tgccttcttg taattcggca ggctttgctg tttccagtaa
    13501 tgcgcccatg ccgcctaacg acaaatcata caggcggaaa cgtaacgtac tgttatccgc
    13561 cagtttggtc tggcaaaaat aaggcggatg gagtggggcg gagatgcgga aatatcggcg
    13621 tcgttgtaca aaccataagg tgggaggcgg tacggtgata aatgccggaa gctgcaagta
    13681 ttcactctgc tgtagttgtt caacagtaaa ctcgactttc gcaccctgag tttcggcggt
    13741 aatggtaatg tgctgtgcct ttagcacggc gatgttgtct tcggcttgac tgccgaaatc
    13801 cagcaccagt ttatccgggg ttattgccag tattttgctg atcagctgcc cgccattcca
    13861 actgagacgc aaaggaattg cggctttgtg caaatcgcgt aacacgccca ggacggctaa
    13921 cggattttgt ttcaggaact gctcatggta atgactcacg cggaaaaact cctgactgat
    13981 aaactgtctt tggtttatcg gtcacagtta acaaaactta atacaaatgc gtgaatattt
    14041 tttacatgtt gttcttaaat cagccgacat acgcccagca tcgatcccgc ccttgcctat
    14101 acttagagcg ttgacgtaag catttcttgc gtccgattca tcgaaacgag ggcctgaaca
    14161 tgggaattat tgcctggatt atttttggcc tgatagccgg cattatcgcc aagctaatca
    14221 tgccggggcg tgatggtggt ggatttttcc tgacctgtat tctcgggata gtcggtgcgg
    14281 tggtcggcgg ctggctggcg accatgtttg gcattggcgg ctccatcagt ggttttaatc
    14341 tgcacagctt cctggtggcg gtggtgggag ctattctcgt tctgggcgta ttccgcctcc
    14401 tgcgaagaga ataagatttt cataaggcgg atagcgatac agatgccgct atccgctttc
    14461 acatcagaac gtatactcga cacctgcttt acgggtggaa caaatcaata tagcacttag
    14521 atgcccgata aagtcggttc ccggtaacaa caacagaatc cttttttaat tattgtttcg
    14581 ttgtttttgg tgtgattcag aaaataaata ttttagacag tagccagtca ggcgttaaaa
    14641 atggggcgga tagtgaggcg gttttggctg cgcttattga acatttagaa ttgatcaatc
    14701 cttctggcag attaagccaa aatacccgtt cggcaatgct tcagttacgc gaagaggaat
    14761 ggtctgaatt ctttttttgg ttgctaaatt ctctggagtg tctggattat gtgattatta
    14821 atttaactcc agaaagcaaa aaaacgttga tgagtgaaca ccgtaacaat attcaggtag
    14881 caatcgatgc tttatatagg caacgtcggt ataaatcacc gggggatgat agtgaaacac
    14941 ttacccgtag aaatgacgct atcttcggca accacgtatg gcaaacattt gcgcaatact
    15001 tcccgccagg acttgaaaaa ccgtctgtgt agaaaacacc atagctatgg tgttatctgc
    15061 cgacaacgcc atccggagaa gccgaatgac gttgtcggag taagcgctgg ttaaggtgtg
    15121 ggttgtgcct ctttggttga gggttgcgtc gttgctgact taacggtcgg acgcgtcgcc
    15181 ggaacattgt cacacggttg ctctttcggg cagatcaaat ccagcatttt cagcgtcacg
    15241 ccattggtcc agccaaagcc atcctgtaat ggatattcgc caccgccgcc ccccgttccg
    15301 gtggtgctga catcatattt ttccaccagc tttttctccc ggtcataggt gtgctgaaca
    15361 ttggtcagga agtgccagct aatgtccatc gccacctctt tttgcccgta gttttgtaat
    15421 ccttctgtcg cgacccactg taacggtgcc cagccatttg gcgcatccca ttgttgccca
    15481 cttttcaccg acgtggtgtt caggccgccg ggttgcagca gatgtgtttt cgtcgccgtc
    15541 gccattttgt tggcgcgatc tttcgctgcc gcattgacgt acagcgggaa cagggcggcc
    15601 gcggttaact gattgcgcac tttatgactt ttcaggtcgt aatcggcata ccagccttgt
    15661 tgatcgttcc acaggtattt ttcgatccct ttttgacggg catttgccag cgtttcgtac
    15721 tggtttgcca tcgcgttatc tccggcagct ttgctggcgc gggcgaggat tttttccatt
    15781 ttaaacatca ggctgttcag atcgaccggt acgatgctgg tggtgcgtaa ggtatttaac
    15841 tgctgcgggt tgtccatcca gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc
    15901 aggtcgcggt aaatttcagt ggcaggtcga ttcggattgc ttttggcggt ggcaatatct
    15961 tccacccatg actctggtcg tggcgtatcg cgatcgtccc agtagcggtt gagaagggta
    16021 ccatcctgaa gtttgacaac gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg
    16081 tccatccagt aagcatattc tttttgcatt tgcggcaggt attgcttcaa cgcggcatcg
    16141 ccttcatgct gcgccagtaa ctctaccatc agggcaaaga agggcggttg cgagcggctt
    16201 aaatagtaac tgcggttgcc gttgggaata tgaccgtaag tgtctatttc atgagcaaaa
    16261 ttggccacca tatccgcgac tttatcccag tgaccgcttt cggcaagtcc taacatggtg
    16321 aagtaactgt cccagtaata tacctcgcga aaacgtccgc ccggcacgac ataaggttcc
    16381 ggcagcggta acagagaatc ccatttttct gtgttttcgg tagaacgcgt taataccggc
    16441 caaagtccgt caatatgttc gcgcagtgac tgcccctctg gcggaacata tttctcgcct
    16501 tctttcggca gggtgaaatt gacgttaacg aaatggcgca gatcaaatcc gctctggttt
    16561 tgctgcatcc gataatcagc aaggatcatc agcggatcgc tgttcggcac ggcatcggca
    16621 aaggtttttt ggtccggaaa aagtttggcg ttttgcacat cattaaacag cggccctaat
    16681 aaaatatcag gcggctgtgg tgttaccggt gtttcttctg cctgcaccga tagcgcagcg
    16741 aaacacaaaa agatacaggc tggaattaac gccatttttt gcgggcgaga aggtgcgggg
    16801 gatttcatca atcattctcc tttggcgaaa ccgaataaaa cggtattcaa gtatcagaaa
    16861 accttagttc aggatcgcac tagctgcatg atcgaaatct cattctgcga actgtcaggc
    16921 attttagaaa agggaattaa agggataaat aacggcgaaa tagagtgatc aaataacgtc
    16981 tgtttattag ccagccagcg gcgttgggtt cgtgattagc gaagaaaaat catcataaat
    17041 tggtgaaaaa tataacaggt agttaaaaac cattagtgct gagtaaattg ccggatgaca
    17101 tcagaacgat gccatccgaa cagtggctta accctgacgg ttgaaacgtt gcgttttaac
    17161 gtccagcgtt agcgtttctt ctggttgtat cgcatacagt ttctcaccct gctggcaaat
    17221 ccagccaatc cccagctcac gggcgattag ggcgctgtgg gaaaccggac ttccggcgct
    17281 aaggcagata ccttttacaa ccgccggatc cagttgcagt actgtggaag gataaatgtt
    17341 ctccgccagt agaatagttg gcgagttaaa ctgcgggagt tcttctttcg tttgggtcag
    17401 gtggaccagg gtgcgatgca gaagatcgtc cacatcaata tagcgagctt gtagatattc
    17461 atcatccagt tgctggtatt gctggctaag ttctttaaga acttgctgcc aggcatattc
    17521 tgccgtgcaa tgttcgtgct gaaggagttc gcttgccgcc gccagcagtt ccggatcatc
    17581 taacagtgta tggtgaccag aaaagattgc ggcaatatcg tcaagcccgc tggcttctgc
    17641 tttcgctgtt aacgtcatca gatctaataa cgtgaagtca atggcctggc gtaatcgttc
    17701 ttgttcctct tccacggtca gggttgattt tgcctgtacc gtacataaaa ccggttgata
    17761 ataaaaggct ttacccgaaa caggcggaac gggacgcaga gtaggtggaa caacttcctc
    17821 cgtttcacca aagttatctt cagccagctg acggaaagcg atcagtgcct cttcagcttc
    17881 tggccctttc gcaatcaggc gcagcgtatc gttatagcga acttgtagta acgcaatctg
    17941 gttaatactc tctggtgtga cgcatttgcc gtttttttcc agcaacatat cggcattaaa
    18001 tgtcgataag gtataaacca gccgggaggc cggacgtaca tgcaggccgt tacggttttt
    18061 tatgaccacc gccagagaac gggcttcttc atcgtacgca ggacatgtgt cagagatttt
    18121 agtgtcggag gacggtaaac ccagttgttc acgtttggct tccagcgcat gcatagcgtc
    18181 aaagataact ttgtcgatat ccgctcccga ggccgcgctg accgttgctg ccagtgtacc
    18241 ttcgaccaac ggcgcagcac acaaacgtac ttttgcggcg atcttgggag ccagcaattc
    18301 cagcgcagtt tcagcactca ataatgcgct acccatatcc atcatgacca gcacatggtc
    18361 ggcatcagca acagattcga tggcctccat cactttgacg gcatcggtac caataggatt
    18421 ttgtggatcg tcaattcccg cggcaatggc gattttacaa ctatcactca ttaacatctg
    18481 acgggctaat tcaccgacac cttctcccag tcggctgcta tgtgaaacta tgaccaggtt
    18541 taccatcacc aattccttac tcttttgcgg ctaacgccaa catttgcatc ataaacatca
    18601 ccgaggtcgc gccgggatcc tggtgaccaa tactgcgttc accgagataa ctggcgcggc
    18661 ctttgcgggc ttgcatcgta atcgtacttt gtgcagcgga ttcggcgatt ctactggcag
    18721 cttcgagcgc caccggaaca gagagatttt gctcgctgga ctgacgtaac gattccacca
    18781 ccggcaccca cacatcacac atggttttat cgccaggttc ggctttcccg cgactgatta
    18841 cgccgtccac gccatcgcgg aacatctgat aaagctcttc cagtgtcagg ctttgccgtg
    18901 cctgggtcgc ctgtgcggcg cggataaaga aggtaccgaa cagcggacca ctggcaccac
    18961 cgacgctgga aagcagcgtc ataccggtat tcttgagaat gaaaccgata tctttatctg
    19021 cgatagcagg gagtttttcc accactttgc taaagcctcg gttcatattt agcccgtggt
    19081 cagcgtcgcc aatttcgcga tccagtccgg taagatactc gctctcggtg ctgaaaatat
    19141 cgccacaacg agtgagccag ttaacaattt gagttctgct cagtgacatt gctttctcct
    19201 tatttacccc agttaagggc cggggtgtgg accggggcgt cccagagtgc cagcgtttcg
    19261 tcatcaactt tcagtaaggt gattgagaaa ccggtcatat ccagtgaggt gcagtacgcg
    19321 ccaattaaat tacgttcgat agtcaatccc gcttgctggc aacgtgtggt caggcggtta
    19381 tagacgccgt acagctcaga aagcggagtt gcgccaagat tgttaaccag cgcaatcacc
    19441 cgatcgccag actggagcgg ttgtttggtt tgttgttctt cctgccaact gccttgttga
    19501 taatcccaga aacgcaaagt gcgatggtat gagccatttt ccagcagggt gtcgaacatt
    19561 tcatcgacgg tttgatcaag ggaagagaag gagcggcggt caatacccgg ctcaccatga
    19621 atgccgacgc caaactccat ctcattatcc gccagggtaa aagaaggttt gcccgcggca
    19681 ggaacggtac aggcaccgag agcgatacct attgagtggc cttgattatt caacttacgc
    19741 cccagttccg cacaggcgtc cagtgagtcg ccacgctccg ccgctgcgcc tacgagtttt
    19801 tcaattaata cggtgttggc aacgccgcgt cgcccggcgg tataaagact gtcttttacc
    19861 gcaacgtcgt catcaatgac cacagtggtc acttttacgc cgctatcgtg cagtaactcg
    19921 gtcgctgttt caaagttaag aatatcgccg gtgtaatttt tgataatcaa cagtacacct
    19981 tcgccgccat caatttgcat ggagcattca aagattttat cgggcgtcgg tgaggtgaaa
    20041 atttcgcccg gacaggcccc cgaaagcatc ccctgaccga tataaccaca gtgcatcggc
    20101 tcgtgtccgc tgccgccacc cgacagcagg gcgacttttc ctgcaacagg ggcatcagct
    20161 cgggtgacat acaccggatc ctgatgcagt gtcagcgatg gatgcgcttt cgccagtcct
    20221 gccagttgtt cgtccagtac gtcttgcaca tcattgatca attttttcat tattttgctc
    20281 cagcaattac ggtagggcat ggatgatgtt caacgacacg gcgaccactg actgccgatg
    20341 aatccattgt gcatcaggca agggaaaaga aaattagccc aaattatgtt tcatagtgaa
    20401 acatatgctt taatgaatgt tccatattga aacttttacg tgtattaata cttaaaattg
    20461 cgagccggaa caccttttgt cataagggat gcgggatatg agtggcgctt ttaacaacga
    20521 tggtcggggc atatctccct taattgcaac ctcctgggag cgatgcaata agctgatgaa
    20581 acgggagaca tggaacgtac cacatcaggc ccagggcgtg acatttgctt ctatttatcg
    20641 gcgtaagaaa gcgatgctga cgctcgggca ggctgcgctg gaagatgcct gggaatatat
    20701 ggcaccgcga gagtgtgcgc tgtttatcct cgatgaaacc gcctgcattc tcagccgtaa
    20761 tggcgatccg caaaccttgc agcagctaag tgcactggga ttcaatgacg gcacgtattg
    20821 cgccgaggga attattggta cttgtgcgct atcgttagcg gctatctctg gtcaggccgt
    20881 gaaaacgatg gccgatcaac atttcaaaca ggcactctgg aactgggcct tttgtgcaac
    20941 gccgttgttt gacagcaagg gccgattgac gggaacaata gcgctggcgt gtccggttga
    21001 gcaaactacc gcagctgatt tgccgttgac gttggcaatc gcccgcgagg tcggaaattt
    21061 actgctgacg gacagtttgc tcgctgaaac taaccgtcat ttaaatcaac ttaatgccct
    21121 gttagaaagt atggatgatg gcgtgattag ctgggacgag cagggtaatt tgcaatttat
    21181 taatgcccag gcggcgcggg tcttgcgcct tgacgcgacg gcaagtcagg gaagggcaat
    21241 cactgaactc ttaacgttac ccgccgtatt gcaacaagca ataaaacagg cacatccgct
    21301 caaacacgta gaagcaacct ttgaaagtca gcatcagttt attgatgcgg tgataaccct
    21361 taaaccgata atagaaacgc agggaaccag ctttattttg ttgctccatc ctgtggaaca
    21421 gatgcggcag ttgatgacca gtcaattagg aaaagtcagc cataccttcg ctcatatgcc
    21481 acaggacgat ccgcaaaccc gccgcttgat tcattttggt cgccaggcgg cgcgcagtag
    21541 ctttcctgtc ctgctttgtg gagaagaggg cgtgggaaag gcactgctaa gtcaggcaat
    21601 tcataatgaa agcgagcgtg ctgcaggtcc ttatatcgcc gtcaattgtg agttatatgg
    21661 tgatgcggcg ctggcggaag aatttattgg tggcgatcgc acggacaatg aaaatggccg
    21721 tctgagtcgg ctggaactgg cacacggcgg cacgctgttt cttgaaaaga ttgaatatct
    21781 ggcggtggag ttacagtctg ctttgcttca ggttatcaag cagggtgtta tcacgcgact
    21841 ggatgcgcgg cgtttaatac cagttgatgt caaagtgatt gccacaacga ccgcggacct
    21901 cgcaatgctg gtggaacaaa atcgttttag tcgccagctg tattacgcgt tgcatgcatt
    21961 tgaaattacc atcccgcctc tgcgtatgcg gcgtggcagc attccggcgc tggtgaataa
    22021 caaattacgc agtcttgaaa aacgcttctc tacgcggctg aaaattgatg acgatgccct
    22081 cgctcgcctg gtttcctgtg catggcctgg caacgatttt gaactttaca gcgtcatcga
    22141 gaatcttgct ctgagtagtg ataatgggcg cattcgcgtc agtgatttgc cggaacatct
    22201 gtttaccgag caggcaacag atgatgtcag cgccacacgc ctttccacca gtctgtcatt
    22261 tgcggaagtt gaaaaagagg caattattaa cgcagcccag gtcacaggcg gtcgcattca
    22321 ggaaatgtcg gctttacttg gaatcggccg cactacgctg tggcggaaaa tgaagcaaca
    22381 tggcattgat gcagggcagt ttaagcgccg ggtatgaaag acagaaacga tttctgatac
    22441 atcagagtga tctatatttc attccggcgc acgctaacat ttttcagcat cgtttaagga
    22501 cttgtctatc ccgcacttaa aagctgaagc gatatcctcc gttgacttgc ttctgatcaa
    22561 agaggttacc ctgcgtgtaa tccgcttcga gatagaatgt gtgctgtttg ttatactgtg
    22621 cactgactcc cacgccatta ttccagccat tacctttgaa actgtacttc tcccgggagt
    22681 tgttcaacag atattctgta tcccctgaaa actcgcggat agcgccagtc ttcacataga
    22741 tattcagctg gctattgcct gcggtgatgt catatcccag tatcatgctg gcacgcccca
    22801 gcagcgattc gtagtgattc agatgtatat tgaggccatt actcgccttc atagccatct
    22861 cattctggtg gctgtatgta agctgggttt gcggctctat atagaaccca taaccagtag
    22921 gggacaggtt gaacctctgc ccggcttcca gggagatgct cattccattc gcagtgccgt
    22981 tggcgttaac gccgttgttc tgactgtcca gtacgtggaa actatttttc tggcgcgatg
    23041 cttttataac gagatcgctg taaaaaccgt tttgtgccat gtaactggcg tacattccca
    23101 tgtagtctga acgtgcggta ccgtcgcctc cgctatagtc cggcgatgca tgtgttgagc
    23161 caatatacag accgacatac aacggcatta catcagagag acgtttatcc ccaccaaact
    23221 ggataccgct gtaacccatg tcaaagccgc tcagtttgcc actggcaaag gagtccaggc
    23281 ttcccccata actgcgcaac cagatattac cgtctttact ctgatttcgc aggtcaccca
    23341 tccgttgcat cagcgtacgg ttttcaacat agttcaataa gtaaccgaca ttgagataat
    23401 tgccgccagc atctgcggtc gtggtgggtt taggcgtggg agcgggttca ggcgtaggat
    23461 cggggttgac tatgggaggc tgagcgggag ttggtgtggg ttcaggatta ggagtcggtt
    23521 ccggaactgt cccggaagcg taaagctccc agttagtgcc atttttacgc acatcgtaca
    23581 gatatccccc caactcaacc tgagaagacg cgctgaacga ggccgcgccg tcagtggttt
    23641 ttaccactgt cagaacttca tttcccgttg tggcctcgct gccctggttg cggatagcca
    23701 atacgtgatt accagcacta ctcccgctga tattcaataa atccccttta ttattaacgc
    23761 cattaccctc gccaacaaca tcagcacgca taataaaggt actgttaccg ctcaggttct
    23821 ctacgtttaa tgtggcaaat gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg
    23881 aatggctcag cgccagcgtg tcgagattag aattacttgt tacgttccag acgctgttat
    23941 tcattgcaac gtccagtttt ccgccattga cattatcgct gagggaggaa cctgtccaaa
    24001 ccgacccagg gtgcatatcc agattgatta gcccaccttt ggaaagaacg ctaccgttga
    24061 taagcatacg acccgaggcg ttgatgcggc tggcggcata accatcgtca tgttgcgttg
    24121 caatggccat ctggtcgggt gtactcatat caatgaccag atcgctcgtg aggtcgatct
    24181 ggctgttggt catggcataa attcccctgg ctcctgccgc gccggtgata gccaaactgt
    24241 ctccggttat tctaccgccg ctgagcgccc acaaacccag cgccagactg ccattacgat
    24301 caactgtaat atcggtattt tgcatgttga caacagccgt tgccgtctgg gccgaggcac
    24361 cataagaacc gccggaaaag atgctgtttc gttgcgctgc cgtgccagta aaattgatta
    24421 tcgcgtctga accactggtg acgaggccac cgccctgcgc ggaagaaata tggctatctg
    24481 caccgatagt ggttgtacca ccacgaactt cgacgccatt ggccgcggct ccagttacat
    24541 caacagtgag tgcattcgcg ctaacctggc caaagctcca gaggccgtga gcattatcgc
    24601 cattggtttt aatggtactg tttgttccga gatcgacaac agagtttttc tgtacgttta
    24661 tccccatggc gctgtagccc tgaacatcga ttgtcaggtc tgtagccgta aaacgcgcag
    24721 caccattggc gttattgccg ttgagaccac cgatataaac acctgtactt ccatcggtcg
    24781 tgattttact tccgcttcca agatcgacac tggtgccata gtcattgatg gttaggccta
    24841 taccgttcga gttttcaatg gtgaattgag tggctgttag tgttgagcta tgcccaataa
    24901 tgatgccgtc atcgttactc ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt
    24961 caccaattaa gttaatgccg atggcagagg tttgaccaac aacatctact gtgagtcggt
    25021 tagctgtcag ggagctgttt ttgccctgaa taatgattcc ttttgaatat ccagaggcgt
    25081 cgttgacatt gacggtgaca tcattaccca ggttgatatt gccaggcgtg tttcctgctg
    25141 gggtcattac accatagagg tttcctgatg gatcggcggt atctcctgta attctgtcac
    25201 catcattaat agtaatattg gtgccatatt gacttcctgt atcagcagct atcagcaggg
    25261 gggaaaagat aattgcattc aaaccaatag cgccaaattt tatcagttga attgagggcg
    25321 aacggatttt taattcagcg agacgatctg ctttggtatt cccattgtgt tgtttgatgc
    25381 ccataccact
//
LOCUS       DAPHDX010000064        25014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPHDX010000064 DAPHDX010000000
VERSION     DAPHDX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.064"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1108
                     /locus_tag="RH058_002529"
     CDS             <1..1108
                     /locus_tag="RH058_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3138104.1"
                     /translation="LSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQ
                     LSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYV
                     VDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLL
                     LLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRY
                     FFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDD
                     ILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFS
                     TGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1289..2779
                     /locus_tag="RH058_002530"
     CDS             1289..2779
                     /locus_tag="RH058_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3138105.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2822..3325
                     /gene="yeaK"
                     /locus_tag="RH058_002531"
     CDS             2822..3325
                     /gene="yeaK"
                     /locus_tag="RH058_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV3138106.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3326..3430)
                     /locus_tag="RH058_002532"
     CDS             complement(3326..3430)
                     /locus_tag="RH058_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138107.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3600..4046
                     /locus_tag="RH058_002533"
     CDS             3600..4046
                     /locus_tag="RH058_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV3138108.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4003..4824)
                     /gene="nimR"
                     /locus_tag="RH058_002534"
     CDS             complement(4003..4824)
                     /gene="nimR"
                     /locus_tag="RH058_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV3138109.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4921..6102
                     /gene="nimT"
                     /locus_tag="RH058_002535"
     CDS             4921..6102
                     /gene="nimT"
                     /locus_tag="RH058_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV3138110.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6157..6504
                     /locus_tag="RH058_002536"
     CDS             6157..6504
                     /locus_tag="RH058_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV3138111.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6526..6780)
                     /gene="yoaF"
                     /locus_tag="RH058_002537"
     CDS             complement(6526..6780)
                     /gene="yoaF"
                     /locus_tag="RH058_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV3138112.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6963..7988
                     /gene="dgcP"
                     /locus_tag="RH058_002538"
     CDS             6963..7988
                     /gene="dgcP"
                     /locus_tag="RH058_002538"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV3138113.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8021..8119
                     /locus_tag="RH058_002539"
     CDS             8021..8119
                     /locus_tag="RH058_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV3138114.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8122..8196
                     /gene="yoaJ"
                     /locus_tag="RH058_002540"
     CDS             8122..8196
                     /gene="yoaJ"
                     /locus_tag="RH058_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV3138115.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8255..8503)
                     /locus_tag="RH058_002541"
     CDS             complement(8255..8503)
                     /locus_tag="RH058_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV3138116.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8730..9938)
                     /locus_tag="RH058_002542"
     CDS             complement(8730..9938)
                     /locus_tag="RH058_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3138117.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            9946..10374
                     /gene="tnpA"
                     /locus_tag="RH058_002543"
                     /pseudo
     CDS             9946..10374
                     /gene="tnpA"
                     /locus_tag="RH058_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10393..10581)
                     /locus_tag="RH058_002544"
     CDS             complement(10393..10581)
                     /locus_tag="RH058_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV3138118.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10585..10944)
                     /gene="yeaR"
                     /locus_tag="RH058_002545"
     CDS             complement(10585..10944)
                     /gene="yeaR"
                     /locus_tag="RH058_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV3138119.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11117..11755)
                     /gene="leuE"
                     /locus_tag="RH058_002546"
     CDS             complement(11117..11755)
                     /gene="leuE"
                     /locus_tag="RH058_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV3138120.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(11882..12805)
                     /gene="dmlR"
                     /locus_tag="RH058_002547"
     CDS             complement(11882..12805)
                     /gene="dmlR"
                     /locus_tag="RH058_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV3138121.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQHFAEHYPNFSLEHA"
     gene            12908..13993
                     /gene="dmlA"
                     /locus_tag="RH058_002548"
     CDS             12908..13993
                     /gene="dmlA"
                     /locus_tag="RH058_002548"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV3138122.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14244..15854
                     /gene="yeaV"
                     /locus_tag="RH058_002549"
     CDS             14244..15854
                     /gene="yeaV"
                     /locus_tag="RH058_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV3138123.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            15886..17010
                     /gene="yeaW"
                     /locus_tag="RH058_002550"
     CDS             15886..17010
                     /gene="yeaW"
                     /locus_tag="RH058_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV3138124.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            17066..18031
                     /gene="yeaX"
                     /locus_tag="RH058_002551"
     CDS             17066..18031
                     /gene="yeaX"
                     /locus_tag="RH058_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV3138125.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18085..19200)
                     /gene="rnd"
                     /locus_tag="RH058_002552"
     CDS             complement(18085..19200)
                     /gene="rnd"
                     /locus_tag="RH058_002552"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV3138126.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19282..20967)
                     /gene="fadD"
                     /locus_tag="RH058_002553"
     CDS             complement(19282..20967)
                     /gene="fadD"
                     /locus_tag="RH058_002553"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV3138127.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21172..21753)
                     /gene="yeaY"
                     /locus_tag="RH058_002554"
     CDS             complement(21172..21753)
                     /gene="yeaY"
                     /locus_tag="RH058_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV3138128.1"
                     /translation="MAVHKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(21793..22488)
                     /gene="tsaB"
                     /locus_tag="RH058_002555"
     CDS             complement(21793..22488)
                     /gene="tsaB"
                     /locus_tag="RH058_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV3138129.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22546..24456)
                     /locus_tag="RH058_002556"
     CDS             complement(22546..24456)
                     /locus_tag="RH058_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV3138130.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24588..24932
                     /locus_tag="RH058_002557"
     CDS             24588..24932
                     /locus_tag="RH058_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV3138131.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         6085 a         6394 c         6080 g         6455 t
ORIGIN      
        1 tttaagcctt gcatttttaa gtggccttat ctatttagtt gaaaccattg taattatcca
       61 taaaccaatt aacggcagta cactgatcca gacaaagtcg aatgatgttt ctattttcta
      121 tattttccgc caactcagtt ttatttgttt aacctcgctg gcactctttt gttatggaaa
      181 agacaacatc cttgacaaca ataagaaaaa aacgggaatc ctgttgctgg cgctgattcc
      241 ttttttagtt tttccccttc tggcacacaa tctgagcagt tataacgctg actattcttt
      301 gtatgtcgtc gactactgtc cggacaacca tactgcgacc tggggaatca actatacaaa
      361 aatattggtt tgtctgtggg catttttact gttctttatt atcatgcgta cacgattagc
      421 cagcgaacta tggccgttaa tagcattatt atgtctggca tcgctatgct gcaacttact
      481 tctactgact ctggatgagt ataattacac catctggtat atcagtcgcg ggattgaagt
      541 ttccagtaaa ctgtttgttg tgtcttttct gatttataac atttttcagg aactgcaact
      601 ctccagcaaa ctggcagttc atgatgtgct gaccaatatt tataatcggc gctacttttt
      661 caacagcgta gagtcattat tgtcgcgacc tgttgttaag gacttctgtg tcatgctggt
      721 tgatattaat cagttcaaac gcatcaatgc ccaatgggga catcgtgtgg gtgataaagt
      781 gctggtttca attgtcgata ttatccagca aagcatccgc cctgatgata ttttagcgcg
      841 actggagggt gaggtgtttg gcttgctatt taccgaactc aatagtgccc aggcaaaaat
      901 cattgcggaa cgtatgcgta aaaatgtcga actcctgacc ggctttagta acagatatga
      961 tgttcctgaa caaatgacca tcagtattgg cacggttttt tcaacgggtg acacgcgtaa
     1021 tatctcgctt gtcatgacgg aagcagataa agccttacgc gaagcgaaaa gcgagggggg
     1081 caacaaagtg attattcatc atatttaagt gcaaaaatat tcagagccat gctttttgcg
     1141 tggcttttgc atacaattta ttactattac ccctaaattt cccctcccct gcggcgcaga
     1201 gatgagtata attagcgccc ctgtgccagg ccgcaatcga actttatctg gttttctcgt
     1261 ttcactaacc gaaggagtgc catttatcat gaaattgcac catagaatgc tccggcattt
     1321 tatcgccgca agtgtcattg tgctgacatc ttccttcctt atttttgaac ttgtcgccag
     1381 cgacagagca atgagtgcct atctgcgcta tatcgtgcag aaagcagact cctcctttct
     1441 ttatgataag tatcagaatc agagtattgc cgcgcatgtg atgcgcgctc tcgctgctga
     1501 gcagtcggaa gtgtcgccag aacagcggcg cgccatctgc gaggcttttg agtctgccaa
     1561 taatacccat ggcttaaacc tgactgccca taaatacccg ggcttacgcg gcacactaca
     1621 aaccgcatcc actgactgcg acacaattgt ggaagctgca gcactattac ccgcttttga
     1681 tcaggcagtg gaaggcaacc gccaccagga tgattacggt tcaggtcttg ggatggccga
     1741 agagaaattt cactattatc tcgatctcaa tgaccgctat gtctattttt atgagccggt
     1801 taatgttgaa tactttgcga tgaataactg gtccttcctg cagtcaggaa gtattggtat
     1861 cgatcgcaaa gatattgaaa aggtatttac cgggcgtacc gtattgtcga gcatttacca
     1921 ggatcagcgt actaaacaga acgtgatgag tttgctgacg ccggtgtatg ttgcagggca
     1981 gctaaaaggg attgtgctgc tggatattaa caaaaacaat ttgcggaata tcttttacac
     2041 tcatgaccgc cctctcctct ggcgttttct caatgtcacg ctaaccgata ccgattcggg
     2101 gcgcgacatt atcatcaacc agagcgaaga taatctgttc cagtatgtca gttacgtcca
     2161 tgacttaccg ggcggcattc gtgtctcgtt atccattgat attctttact ttatcacgtc
     2221 ttcgtggaaa agcgttctgt tctggatttt gactgcgtta attttgctga atatggtccg
     2281 gatgcacttc cgtttatacc aaaatgtgtc gcgagaaaat attagtgatg cgatgactgg
     2341 actgtataat cgcaaaattt taacccctga actggaacag cggttgcaga aactggtgca
     2401 atccggttct tcggtgatgt ttattgcaat tgacatggac aagttaaagc taataaatga
     2461 caccctcggt catcaggaag gggatttagc gattacgttg ttagctcagg cgattaaaca
     2521 atcgattcgt aaaagtgatt atgccatccg actcggtggc gatgaattct gcatcattct
     2581 tgtcgattcg acgccgcaaa ttgcagcaca actgcctgaa cgtatcgaaa aacgtctgca
     2641 acatatcgcg ccgcagaaag aaatcggctt ctcttccggt atttacgcga tgaaagaaaa
     2701 cgatacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa
     2761 caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga
     2821 aatgactgaa atggctaaag gaagcgtgac gcatcagcgg ttaatcgctt tattatcaca
     2881 agaaggtgct aacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc
     2941 agaaattcgt ggcaccgctt taggccaggg agctaaggca ctggtatgta aagtcaaagg
     3001 caatggcgta aatcaacatg ttctggcgat cctcgctgcc gatcaacagg ccgatctgag
     3061 ccaacttgcc agccatatag gcggattacg cgcttcgttg gccagcccgg cggaagtcga
     3121 tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa
     3181 actggttgcc gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat
     3241 gctggataaa tccgttattt tgaaaaccac ggattatttg cgcattgcgc aaccagaact
     3301 gctcaatttc cgccgcactg cgtaactagc ctgtccgttc aataagcaga acggacaaaa
     3361 ccagcacaac agcaatggca aaaaacgatg acgtgataat cagtgtctcg acgaacattt
     3421 gatcgttcat agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa
     3481 atggcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag attttctact
     3541 tccttcctgc agcaagcgta aagtaagcag gcttattatt ttttggcaag gaaaccacga
     3601 tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt atcagtcata
     3661 acaccactgt cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca
     3721 cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt ctgacgattg
     3781 gcgttatggc ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc
     3841 tgaactggaa atcgctggtg gcaattgcag taggcgttat tgtctcctgg ctgggtgggc
     3901 gcggcgtgac cttaatgggc agccagccgc aactggtcgc cgggctgctg gtaggcactg
     3961 ttttaggcgt ggcgctgttt cgcggcgtac cggtcggccc gcttattgcc gcgggtctgg
     4021 tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc
     4081 caggcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc
     4141 aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca gctgtaactg
     4201 ctgacgccac tggcgaaaac tcaaccccgt ctctttcact atcagccggg caagattgcg
     4261 ttcactcatc gcaaaaaagc cagcccattg ccccaatgag ccccactcga cgggctcttt
     4321 cgccatcatc tccaccatcg tgcggatttt aggatgagaa gaaacgggca agtgcagctg
     4381 ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc gggtcatcgg
     4441 ctcagcacgc tgagtcgtcg tcctgtttgc cagggttaaa atcaattccc ggcataaggg
     4501 cgatattttc aatgtgcaac atgttgtcgg cattgtcact gcgctgggtt cgataaacag
     4561 aaaacagagt tcagcgttag ctgtcacttg attgctatgt tccaccccgc ccggtatcca
     4621 aaccgcatat tgcggcggca ccatccacaa agcattttcc accgtacagg taattgcacc
     4681 atgtaatgct agtatcagct gtcctttgcg gtgctgatgt acaggactgg ttaactcatc
     4741 cgttccggcg tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg gttcatagcc
     4801 attgagattc aacctgtgca tcattttgtc cgaatttagc gataatttgt cattttagct
     4861 tgattcaaca taacaataaa aacggtaagg tacagcctcg tttgtaacaa tgagaagcat
     4921 atgacctgtt caacttcatt aagcggcaaa aacaggattg tccttatcgc tggcattctg
     4981 atgattgcca ctacattacg cgtcaccttc accggcgcag caccgttact cgatacgatt
     5041 cgttccgctt actcgctgac gacagcgcaa accggcttat taaccaccct gccattattg
     5101 gcctttgcgc taatctcacc tttggctgcc ccggtggcgc gacgttttgg tatggaacgt
     5161 agcctgtttg ccgcgttact tttgatctgt gcaggtatcg caattcgttc tctcccttcg
     5221 ccttacttat tatttggcgg tacagcggtc attggcggtg ggattgcatt aggcaatgtc
     5281 ttactgccag gattaattaa acgcgatttc cctcattccg tcgccagact taccggcgca
     5341 tattccctga caatgggagc tgcagcggca ctgggatcgg ctatggtcgt gccgctggct
     5401 ttgaacggtt ttggctggca aggcgcgttg ttcatgctga tgtgttttcc tctgctggct
     5461 ctttttttat ggctgccaca gtggcgaagc caacaacatg caaatttgag tacctcgcgc
     5521 gccttacata ctcggggtat ctggcgttcg ccgcttgcct ggcaggtcac attgtttctt
     5581 gggatcaact cactggtcta ttacgtgatt attggctggc ttccggcgat cctcatcagt
     5641 cgcggctata gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact agccacagca
     5701 gctcccggtt tgctgatccc tcttttctta catcatgtga aagatcagcg tggtattgca
     5761 gcgttcgttg ccttgatgtg cgcagtgggc gctgttgggc tctgctttat gccagcgcac
     5821 gcgatcacct ggactctgct tttcggtttt ggttccggcg caacaatgat actggggttg
     5881 acgttcattg gtcttcgggc aagttctgcg catcaggcgg cggcactctc ggggatggca
     5941 caatccgtcg ggtatttgct ggcagcctgt gggccgccgc tgatgggtaa aatacacgat
     6001 gctaacggtg actggtccgt accactgatg ggtgttgcca tactttcact actgatggcg
     6061 attttcggac tttgcgccgg gagagacaaa gaaattcgct aatatccggt gctatagtga
     6121 cgtgacaaat catgcgtgaa agggagaaca aacacgatga atattcagtg caaacgcgtt
     6181 tatgatccgg ctgaacagag cgatggttat cgcgtactgg tcgaccgcct ctggccgcgc
     6241 ggtatcaaaa aaaccgattt agcccttgat gagtgggata aagaaatcac gccgtcaacg
     6301 gaactgcgca aagcctttca cggcgaagtc gtcgattttg caacctttcg cgagcaatat
     6361 cttgcagaac tggcgcaaca cgagcaagaa ggaaagcggc tggcggacat cgccaaaaaa
     6421 cagccgctga ccctgctcta cgcagcaaaa aacaccacgc agaaccatgc gctggtgctg
     6481 gccgactggc tacgtagctt gtgattttag tacagcatcc ggcggttatt tttcaccagc
     6541 cggatggtcc cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg gtaatttgca
     6601 attgttgctg accccttgcg ctgtctgcac tggaatgagc gtcccgccct tctgctggca
     6661 atagaccgac gccggatttg ccataccaat ctgcggcggt ttaggtgctt ctggctgaga
     6721 aggggttgaa caaccagcca ggaccagcaa gcaaggcaga acaaaactga taattttcat
     6781 ttattgatct cacatattta tccagactta gagtatcgcg gtatcgtttt gttttgcagc
     6841 actattttta ttacattcac tcaaatcata ttacgccttg tttcatcttt gttgatgatg
     6901 ttttatcatg cctgcaaaga ttaaataatc agcatttacc cgccgtatcc tggagttgtt
     6961 tcgtgtcaga tcagattatc gcccgcgtct cgcaatccct tgccaaagaa cagtcactgg
     7021 aaagtctggt ccgacagctt ctggagatgc tggaaatggt cactgatatg gaatcaacct
     7081 acctgaccaa agtggatgtc gaagcgcgcc tgcagcatat tatgtttgcc cgtaacagcc
     7141 agaaaatgca catcccggag aattttaccg tctcgtggga ttactcgtta tgcaaacgcg
     7201 ctattgatga aaactgcttt ttcagcgatg aagtccctga ccgttggggc gactgtattg
     7261 cggcacgcaa tcttggaatc accacatttc tgagtacacc aattcactta ccggatggat
     7321 cattctatgg cacgctttgc gccgccagca gtgagaagcg ccagtggagt gaacgcgcgg
     7381 aacaggtttt acagttattc gccggactga ttgcacaata tattcaaaaa gaggcgctgg
     7441 ttgaacagct gcgcgaagcc aatgccgcgc tgattgcgca atcgtatacc gactcgttaa
     7501 ccgggctacc gaatcggcgg gcgatttttg aaaatctgac gacgctgttt tctctcgccc
     7561 ggcatcttaa ccataagata atgatcgcgt ttatcgatct ggataacttc aaattaatca
     7621 atgaccgttt tggtcataat agtggcgatc tgtttctcat tcaggttggc gagcgcctta
     7681 atacgctcca gcaaaatggc gaagttattg gtcgtctcgg cggtgatgag tttttggttg
     7741 tttcactaaa caacgagaat gcggatattt cgtcgctgcg agaacgtatt caacagcaaa
     7801 tacgtggaga atatcactta ggtgatgttg atttgtatta tcccggtgcc agtcttggca
     7861 tagtagaagt cgatcctgaa acaaccgatg cagacagtgc cctgcatgct gccgatattg
     7921 cgatgtatca ggagaaaaaa cacaaacaga aaacaccttt tgtcgcgcat ccagcgctac
     7981 attcctgagg cgtattcaca tccttttgat tggtgataac atgcgaatcg gtattatttt
     8041 tccggttgta atctttatta cagcggtcgt atttttagca tggtttttta ttggcggcta
     8101 tgctgccccg ggagcataaa gatgaaaaaa acaacgatta ttatgatggg tgtggcgatt
     8161 attgtcgttc tcggcactga gctgggatgg tggtaacgtc acctctaaaa aatagcaaag
     8221 gctgcctgtg tgcagccttt gtgcaattta agcgttaact tttaatcttc ctgtagataa
     8281 atagcacgac aatcgcacca ataacggcaa ccacgaagct gccaaaattg aagccatcga
     8341 ctttaccaaa gccaaacagt gtgctgatcc atccgccgac tacggcaccg actatcccca
     8401 gcagaatagt cataaagaat ccaccaccat ctttacctgg catgatccac ttcgccagaa
     8461 taccggcaat aagcccaaaa ataatccatg acagaatgcc cattgtttcc tcacttatct
     8521 gttttgcatt agcgggttag tcgctgataa aaagcatagc acaacatcgg gagggcaaga
     8581 tttgtgacga gcatcacgga ggattttttt gcgatggcgc agaaattgcg ccatcaacga
     8641 tcggtgataa ttaccaacca caaacatcat gttcgttttc cgtgtcttga catcctccac
     8701 gccctgaatg acgaggatcc ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt
     8761 tcctgcttca acgagcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc
     8821 gccgctaaaa tgttacgagc gccgtttaca tcggcgttcg ctgtatatcc acatacctgg
     8881 catctgaatt gactttgtga caggcgattt tctttcgctg tatgaccaca gcacgcgcaa
     8941 cgctggcttg tgtacgctgg cggaacagca agcacctgac cgccactcca gagctgcttg
     9001 tactcaagct ggcggcgcat ttcataccag ccctgatcca gtatcgaacg gtttaaacct
     9061 gattttgccc ggacattgcg ccccggctgg ctgaccgtac ccgctgctga ctttgacatg
     9121 ttgctgacct tcaaatcctc aatgactatc attgcgtggt ttttgctgac ggtcgttgtg
     9181 actttatgaa ggtagtccct gcggatattt gcgatacggg aatgcagtcg ctgtattttg
     9241 cgtttctgct tctgccagtt attgctgaat ttgaccttgc ggcttaactg tcgctgaagt
     9301 ctcgccagcg tcttctggtt tttctgaaaa ctgtttacag gcccaaagac tgtgccatct
     9361 gacagcgtgg cgagtttagc cacgccagca tccagcccga tcattgatgc tgaagggtga
     9421 accggagttg atacttcact ttctgtctga atactgatgt accacttacc gcaggactgg
     9481 ctgaccgtga catttttcac aacacccgtg acctgacggc tattccggta gcgcatccag
     9541 cccagtttcg gcagaaaaat acggctgttt tcctgatcga gctttacgcc ctgcgggtag
     9601 cggaatgcat cattctgtcc ccgctttttg aatcggggaa aagcagcccg cttccggaag
     9661 aagtttttgt aagcccgctc aaggtctttc agtgactgtt gcaatggctg tgagggcgaa
     9721 tctttaagcc attgcgtttc agtggcgttt ttccactcaa ccagccagga agccattttc
     9781 ccgtaaggga tgtatttatt accggcctca tgattctcat tctgacgtgc cagagcacga
     9841 ttgaaaacga aacgacatgc gcctgcgaag cgcctcattt cacactcctg ttgaccacct
     9901 ggtcttaact ggaatttaaa tgcctgtaat cgtttcacag gtcaaatgat actttggtct
     9961 gtgaaaaaag aaaccgatat ccggcgtgga aggcattgtg ttttcctgat gcatgttcac
    10021 ctggtctttg tcaccagata ccgactccag atttttgacc atgacgcgac agaaaaacta
    10081 cgcacttact tttcaaatgt atgtgctgat tttgaagctg aacttgttga aatggatggc
    10141 gaaccagatc acgtccattt gttaattaac tatcctccca aactggcgat atccagtctg
    10201 gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac gagatcgacc agatattgca
    10261 gtcaggtatt actacaaagg cgttttgtgg agtcctggct attttgccag tagctgcgga
    10321 ggtgcgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc tggtcaggtg
    10381 gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt ttacggcgca ccggataaga
    10441 acgcacggta ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt
    10501 catcggcgtc tctgtttcat aatcgacaga aacgccattg ctgttattgg tgacggtcac
    10561 ggtatacgtt gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc
    10621 accttccatc aggacttcag gagccacgaa gaagtcaatg ttgaaataag tatcgtcagt
    10681 catggcttca atgttgtgcc acttttctgg agggaacacc gcaaactgcc ccgcttcgat
    10741 aaggatcacc tgatcaggct ctgcactgtg ttcatcagcg tagccgagat atttgaccgc
    10801 cccatgcata acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg
    10861 ttcgaatatt ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat
    10921 ataattctgt gggatttgca gcatcctatt ccctccttcg gggaatgcgc tgaaaacggt
    10981 ttattccagc cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt
    11041 actttttagc accatatcag agaaaaatca gggaggatta caagtgggaa gcccgcgaca
    11101 acgcgggctg aatgtatcag gattgcagcg tcgccagtcg ggcggcgaaa cccacgaaca
    11161 tcaaaccaat cagtgagttg ccaactttag ccagtttctt tttggtacgt atgtactgcg
    11221 tgacaaaagc accagaaata atcaggaagc tcaaatagca gaaactcacc agttccagcg
    11281 tcgtcgccag aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta
    11341 cgaaaaacga cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc
    11401 gtttaaaaat ggccccgtat tggggctcat cggatttggc ctcgttattt ttacccttca
    11461 gggtcgcgta gagaatttta ctccccagat agagcaaata aaacgcacca agataacgta
    11521 caatgttgaa taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa
    11581 acatcaatac tgcatcgcca ataaatacac cgcaggccgc aagataaccg cctttcatac
    11641 cgctactgac gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa
    11701 aaatggcccc aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa
    11761 cctctttaat tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag
    11821 cggcttagaa aggagaaaat caggttttaa cccgatatca acccgataat tgaatcatta
    11881 atcaggcatg ctccagtgaa aaattcgggt agtgctcggc aaaatgctgg cgtaaaaatt
    11941 ctaccgttat ccgcactttc gctgacgtcg ccagccttga aacataaacg gcccagacgt
    12001 tcgctggctg gtaatattcc ggtagcactt gcactaaatg accactggca atgttttcgc
    12061 taacatccca ccaggagcgc agagcaatcc cctgcccatc gagacaccac tgatggacaa
    12121 tttctccatg attagacgac agtgggccgg tgactttaat cgcatggtgg ccttctttat
    12181 tgcgtaattg ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg
    12241 cggaaagatc ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggcgacgcgc
    12301 agagaatgcg gtaattagtc gccagtttgc gggcgattaa attaggggcg atgtcatcgc
    12361 caatgcgaat atcgagatca acaccttcat tgaccaaatc gaccaaccgg tcttccacat
    12421 caaaacgtaa ttccagttgc ggatacgcct tagccagcgc tgataacgcc ggagccacca
    12481 cctgtcgtcc aaaaccaaag ctgctgataa tacgcagcat cccctgcggc acctgacgca
    12541 cgtcagaaag ttcgtccatc atctgaccga catcctgcaa aatccgctgc gcccattcat
    12601 aaatccgctc tccttcttcg gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca
    12661 cgtttagcgt ttgctccagc aaggcgatgc gcttgctgac gaacgccggt gaaacgccca
    12721 gttcttccgc cacggcggca aaaccagccc gacgagccac cagcataaag acgcgcaaat
    12781 cattcagcag cggtaaatta ttcatgattc gtgttttatg tttcaccagt tacggggatt
    12841 aattcctttt cagtcaatta taggatggtg atgttgtcaa ttttgatggt caggaagtga
    12901 gaacccaatg atgaaaacga tgcgtattgc tgcgatcccg ggagacggga ttggcaaaga
    12961 agtccttcct gaagggattc gcgtgttaca ggctgccgct gagcgctggg gcttcgcctt
    13021 gagttttgag caaatggagt gggcgagctg cgagtattac agccatcacg gtaaaatgat
    13081 gccggacgac tggcatgagc aacttagccg tttcgacgcc atctattttg gtgccgtcgg
    13141 ctggccggac accgttcctg accatatttc gttgtggggt tcgctgctga aatttcgtcg
    13201 tgaattcgac cagtacgtca acctgcgccc ggttcgtctc tttcctggcg ttccctgccc
    13261 gctggcggga aaacagccag gcgacatcga tttttacgtg gtcagggaaa acaccgaagg
    13321 cgaatattcc tcgctcggcg gtagagtgaa tgaaggtaca gagcatgaag tcgtcattca
    13381 ggaatcggta tttacgcgtc gtggtgtcga tcgcattttg cgttatgcct tcgaacttgc
    13441 gcaaagccgc ccacgtaaga cgctaacttc tgccactaaa tcaaacggtt tagccatcag
    13501 catgccgtac tgggatgagc gagtggaagc aatggccgag aattacccgg agatccgctg
    13561 ggacaagcag catattgata ttctctgcgc gcgttttgtg atgcagccgg aacgcttcga
    13621 tgtggtggtg gcgtccaatt tatttggcga tatcctttcc gatcttggcc cggcctgcac
    13681 cggcaccatt ggcattgccc catccgccaa cctgaatccg gaacgcactt tcccgtcgct
    13741 cttcgagcca gtccacggtt ccgcgccgga tatctacggg aaaaacattg ctaaccctat
    13801 cgccacgatt tgggccggtg caatgatgct cgattttctc ggcaatggcg atgagcgttt
    13861 ccagcaagcg cataacggta ttctggcagc aattgaagaa gtgattgctc acgggccgaa
    13921 aacacctgat atgaaaggca atgccaccac gccacaggtt gccgacgcga tttgcaaaat
    13981 tattttgcgt taaggtcaaa ccagtttatt tgaaccgcgt cactgacgcg gtttttttat
    14041 tcgttctttg cagtaaataa cctgcgtcat ttcacctttt attgtttccg tttcgtgttt
    14101 tatggctttc cgtattctta attgtttaat ttatgtaaca tgcaaataat caatttgtta
    14161 aacacaacat acaactcatt accctgcaaa taattacaac tggcaaaagc cagagacggg
    14221 cttttatgcc caggagatga ctcatgatga gcaatgttaa gaaaaaagat gtgccgctga
    14281 taagcatcag cctggtggcc attcttttca tcgcagctgc attaagcctt ttcccacaac
    14341 aatcggccga cgcggccaac gcaatataca cttttgttac gcgtacgtta ggttccgccg
    14401 tacaggtatt ggttttgctg gcaatgggac tggtgattta tttagccacc agtaaatacg
    14461 gcaatattcg tcttggcgaa ggaaaaccgg aatacagcac gctctcctgg ctgtttatgt
    14521 ttatttgtgc cggtttaggt tcttctacgc tttattgggg ggttgctgaa tgggcctatt
    14581 attatcaaac gcctggatta aatatcgcac cgcgttcaca acaggcactc gaatttagcg
    14641 tcccttactc cttcttccac tggggcatca gcgcctgggc aacttatacg ctggcctcat
    14701 taatcatggc ttatcacttt catgtgcgta aaaacaaagg tctgagtctt tccggcatta
    14761 ttgccgcgat taccggcgtt cgcccgcaag gcccatgggg aaaactggtt gatttgatgt
    14821 tcctgatcgc cactgtcggc gcactgacta tttcccttgt tgtcaccgca gcaaccttta
    14881 ctcgtggact ttccgcgctg accggtttac ccgataactt caccgtgcag gcatttgtga
    14941 tcctgctttc cggcggcatt ttttgcctaa gctcgtggat tggtatcaac aacggtttgc
    15001 aacgtctgag caaaatggtt ggctggggcg cgttcctgct gccattactg gtgctgattg
    15061 tcggcccaac cgaatttatt accaacagca tcatcaatgc catcggtctg accacgcaaa
    15121 acttcctgca aatgagctta ttcaccgatc cgcttggcga tggttcattt acccgcaact
    15181 ggaccgtttt ctactggctg tggtggatct catacacccc tggcgtagca atgtttgtca
    15241 cccgcgtttc ccgcggtcgt aagattaaag aagttatctg gggactgatc ctcggcagca
    15301 ccgtcggttg ctggttcttc ttcggcgtaa tggaaagcta tgccattcat cagtttatca
    15361 atggcgtaat caacgtccca caggtgctgg aaacactggg cggcgaaaca gctgtacagc
    15421 aagttctgat gtcgttgcca gccggtaaat tgttcctcgc cgcatacctg ggcgtgatga
    15481 ttattttcct tgcctcgcat atggatgcgg tggcctacac catggctgcg accagtacgc
    15541 gtaatctcca ggaaggtgac gatcctgacc gtgggctgcg tcttttctgg tgcgtggtga
    15601 tcactctgat cccgctttcc atcttgttta ccggtgcttc gctggaaacg atgaaaacca
    15661 ccgtcgtgct cacagccctt cccttcctcg tcattttact ggtgaaagtc ggcggattta
    15721 ttcgctggct gaaacaggat tacgccgaca ttccggctca tcaagttgaa cattatctcc
    15781 cgcagacacc ggttgaagcc ctggaaaaaa cgccagtgct ccctgcggga accgtattca
    15841 aaggcgacaa ctgagcgcca tcatcctaac gataaaggta tccctatgag caatctgagc
    15901 cctgactttg tactacccga aaatttttgc gctaacccgc aagaggcgtg gaccattcct
    15961 gcccgttttt ataccgatca gaacgcgttt gaacacgaaa aagagaacgt cttcgccaaa
    16021 agctggattt gcgtcgctca cagcagcgaa ctggcgaatg ccaatgatta tgtgacgcgt
    16081 gagatcattg gcgaaagcat cgtgctggta cgcggtcgtg ataaggtttt gcgcgcgttc
    16141 tataacgtgt gtccgcaccg tggtcatcag ttgttgagcg gtgaaggaaa agcaaaaaat
    16201 gtgattacct gcccgtatca cgcatgggca ttcaaactcg atggcaatct ggcccatgca
    16261 cgtaactgcg aaaacgtcgc caatttcgat agcgacaaag cgcaactggt tccggtgcgt
    16321 ctggaagaat atgccggatt cgtcttcatc aacatggacc ccaacgccac cagcgtagaa
    16381 gatcagttac ccggtctggg cgcgaaagtg ctggaagcct gcccggaagt ccacgatctg
    16441 aaactggcgg cccgctttac cacccgcacg cctgccaact ggaagaacat tgtcgataac
    16501 tatctcgagt gctatcactg tggtccggcg catccaggtt tctccgactc cgtacaggtt
    16561 gatcgttact ggcacaccat gcacggtaac tggacgctgc aatacggttt cgccaaaccg
    16621 tccgaacagt cgtttaaatt tgaagagggt acggatgcgg cattccacgg tttctggctg
    16681 tggccgtgca cgatgctgaa cgtcaccccg atcaaaggga tgatgacggt catttatgaa
    16741 ttcccggtgg attctgaaac taccctgcaa aactacgata tttacttcac caatgaagag
    16801 ttaaccgacg agcaaaaatc gctgattgag tggtatcgcg atgtgttccg tccggaagat
    16861 ttacgtctgg ttgaaagcgt acagaaaggg ctgaaatcgc gtggctatcg tggtcagggg
    16921 cgcatcatgg ccgacagtag cggtagtggc atttccgaac atggtatcgc ccatttccat
    16981 aatctgctgg cgcaggtgtt taaggactaa tgacatcggc ggcggtattt tccgccgctg
    17041 ggctgatttt tgatggagta cagcaatgtc agactatcaa atgtttgaag tacaggtgag
    17101 tcaggtggaa ccgcttaccg aacaggtgaa acgcttcacg ctggtggcaa ccgatggcaa
    17161 accgctgcct gcgttcaccg gaggaagtca catcattgtg cagatgagcg atggtgataa
    17221 ccagtacagc aatgcgtatt cactactgag ttcgccgcat gacacctcat gttatcagat
    17281 tgccgttcgg ctggaggaaa actcgcgcgg cggttcccgc tttttgcatc agcaggtaaa
    17341 agtgggcgat cggttaacga tttcaacgcc taataacctg tttgcgctaa ttccctcagc
    17401 cagaaagcat ctgtttatcg cgggcggtat tggtatcacc cctttcctgt cgcacatggc
    17461 agagctgcaa cacagcgacg tcgactggca gctacattac tgctcgcgaa atccagaaag
    17521 ttgcgcattt cgtgatgagc tagtccagca tccgcaggct gagaaagtcc atttgcatca
    17581 ttcatcaacc ggaacacgac tggaattagc gcgattattg gcggatatcg aacctggcac
    17641 acacgtttat acctgtggcc ccgaggcgct aattgaagcg gtaagaagtg aagctgcgcg
    17701 tctggacatc gccgccgata cgctgcactt tgagcaattt gctatcgaag acaaaaccgg
    17761 cgatgcattt accctggtgc ttgcccgttc cggaaaagag tttgtggtgc cggaagagat
    17821 gactattttg caggttattg aaaataataa agccgcgaaa gtggaatgtt tatgtcgtga
    17881 aggggtatgc ggaacctgcg aaacagcaat actggaaggt gaagctgacc atcgggatca
    17941 atattttagc gatgaagagc gtgccagcca gcaaagtatg ttgatctgtt gttcgcgtgc
    18001 gaagggtaaa cgcctggtgt tggatttgta gtttgccgaa gccggatgtg gcgctgagcg
    18061 cgcccagtcc ggcttcggaa gattttactg cggatattcc tgcaataaat tgtgtaatgc
    18121 ttccgccatc agctcaccac gccagccgga aatcagctcc ggcaaattgt tctgcggttt
    18181 cagtttccag tgccagttca gcagttggtt gatttgccga cgcgatgcca gcaattcggc
    18241 gctgatctta tgcgtttcgc tcacgtcagt aatcagcgac ttaatcgctt taaacgcttt
    18301 acgataaccc ggcatgtcca tcaggttaag catcggctgc ggtaaggcct cttccggcaa
    18361 tgtctgcgct ttttccacca gcgccagcag cgttttaccg tgaaagcgga tttcgctacc
    18421 ggataaaccc aggctgtcca gttcgcctaa acttcccggc atataacgcg ctaccgacca
    18481 caaatgctct tcacgcacga caaagttcac cgccagatcg cgctctcgcg ccttgcgcag
    18541 tcgccagtcg gctaacagtt gcagacaggc cagttggcgt gtgcgtaatt gccaggcatt
    18601 ggtgatatca cgccaggcat cttccggcgc aacgacttcc tgacgacgca tttgcatcag
    18661 gcggcattca tccagcgccg caggtagcca gccggaggcc tccgtttcta ccataagctt
    18721 ggcggtgatc ggtaacagat accagacatc cgccgctgcg tattcacact gacgttcggt
    18781 cagcggtctg gccagccagt cggtgcgcga ttcactcttg tccagcgtaa cgccggaata
    18841 ctcttccacc atggaagcga aaccccatga catcgggcgt ccgcagaagg cagcaaggat
    18901 ttgcgtgtca atcaagggtt gtggtaattc gccaaagaca ttgaggaaca cttccagatc
    18961 ttcactgcct gcatggagaa attttgtgat ggacggatcg cgcaggatcg ctttcagcgg
    19021 tgaccagtcg gtgatcccga gtggatcgat tagcgccaga tgctcgccat cgaaaagttg
    19081 aatcaacccc agctgcgggt aataagtgcg cgtacgaaca aattcagtat ccagggctat
    19141 cgccggaaag gcacggacgg cttcacacaa agaagccagc gcatcgtccg tggtaatcat
    19201 ttggtaattc aaattgtttt ctctttagtg ggcgtcaaaa aaaacgccgg attaaccggc
    19261 gtctgacgac tgacttaacg ctcaggcttt attgtccact ttgccgcgcg cttcgtcacg
    19321 taattctcgt cgcaaaattt ttccgacgtt agatttcggt aactcatcac gaaactccac
    19381 cagcttcggt actttgtatc ccgtgagctg acggcggcaa aaagtcacca gtgactcttc
    19441 ggtaagcgat ggatcttttt tcactacgaa gattttcacc gcttcaccac tggagccgga
    19501 aggtacgcca acagccgcga cttcctgtac gccaggatgc tgcatgacga catcttcaat
    19561 ctcgttggga tagacgttaa aaccggaaac cagaatcatg tcttttttac gatcgacaat
    19621 gcgcaggaat ccttcttcat ccattaccgc gatgtcgccg gtgtgtaacc agccattttt
    19681 gatgatttca tcggtagcat cgggacgctg ccagtaaccc agcatcacct gcggtccttt
    19741 gacacaaagc tcacccggtt gacctggtgg tacttcatta tcatcatcat ccaccagttt
    19801 ggcttccgtc gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg
    19861 gttaacgctg accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc
    19921 ggtcagtttc acccaacgct ctgccaccac ttgctgcact ggcatcccac cgcctgcgga
    19981 aagatgcaga ctggagaaat ccagctgctg gaactcttta ttgttcagca acgcattgaa
    20041 caaggtgtta acgccagtga tagcggtaaa cggatatttc gctaactctt ttaccaaccc
    20101 tggaatatca cgcgggttag tgataagcag gttctgccca cccagttcga taaacagcag
    20161 gcagttaatg gtcagggcaa aaatgtgata cagcggcagt gccgttacta ccagttcttt
    20221 acctggatgc agcaacggcc cataggtggc gttgacctgt tccaggttcg ccagcatatt
    20281 gcggtgagtc agcatcgcgc ctttcgccac acctgtggtg ccgccggtgt attgcaggaa
    20341 ggctaaatct tccggcacca gttcgggttt gacgtactgc atccggtagc cgttatgcag
    20401 tgcgctacga aatgaaatgg catctggcag atggtatttc ggcaccaaac gcttgatgta
    20461 tttaacaacg aaattgacta ccgtgccttt tgccgtagat agctgatcgc ccatacgggt
    20521 cagaattacg tgctgaacgg cggttttatc aaccactttt tccagtgtgt gagcaaagtt
    20581 agacacgata acaatcgccg atgcgccgct atcgttaagc tgatgctcaa gctcacgcgg
    20641 ggtatacaac gggttaacgt ttacgacgat catcccggca cgcaaaatgc caaacagcgc
    20701 caccggatat tgcaataaat taggcatcat caacgcaacg cgatcgcctt tcttcagccc
    20761 caacccttgt tgcaaataag cggcaaacgc gcgactgcgt tcttccagct tgcggaaggt
    20821 cattacctcc cccatattca caaacgcagg ctgatcggcg tagcgcgcga ccgactgctc
    20881 aaacatatct accagagatt gataacggtc agggttgatc tccgtcggaa cgtccgcggg
    20941 ataacggtta agccaaacct tcttcaattc ttcacctcta aaatgcgtgt tcgtcgtcat
    21001 cgcaacccca aatgatatac atgccgttaa cataatatta actcatcata ccagcttgat
    21061 aattacccaa cgaaaaggtt gcgaagcgcg tcactattta tttttatctt taccgtaaga
    21121 atgcagaaac agcggaccag ccgctgtttc tttttcttta aaaacaagca attattcagt
    21181 tacaactgtt tgtactctcg cggggccggg attataccag ccccatccgc cgtagccata
    21241 gggccagcca cgaccgccat aaaaccatgg atcaatcggc tgaggcggca taatcacctg
    21301 ctgggttaaa tgccaacgtt tgtaacccgt tacttgcatc accataaatt tatagggcgt
    21361 attgccgatt ttgccgtcaa ccgcaccagt gattggcccg actaccgtaa ccagttgtcc
    21421 acggaaatcc accgggtcca gaaaaccgtt cacatcggca taaatgcgac cgcgagaagg
    21481 ttctcccagc gtcggtctgg ctccgctgtc cagcggtacg gtagcaattt ccaggcgggt
    21541 tttcccttgc tggttttgta ccgcaaccac tttgccacca aagcgtgcct cctgaccaac
    21601 gtacagctgc ggcgcactca tcacccgaac taaatcttgt tgcggcgtgg gactgctgcc
    21661 tttaatggcg tccggcacag tgacacaacc gctcagcatt agcgcaaacg tacctgccag
    21721 tatgcctttg ataacattct tatgaaccgc catgatgcga ctcctttttt tctcagggca
    21781 tactcttaag attcattctt tgcccggaag tttcttccat gcgacgttgt tacgcaaata
    21841 aaccggctcg gcatgttcca ccgccaccgt tttaccctcg gcaaatatct gacaagcaat
    21901 cggcagcata tcttctgcag cgggcagtaa cacttctccg tcgcgcaaaa ccagcccgct
    21961 ctctttaccg agatccggcc aggcttgcca gcccgtccct accgttaccc attcgccgga
    22021 aagctgttgc attcgttcat gaacgagttc gggtttgagt acggcttcgg tttcttcacc
    22081 gtgccagata ccgttttcat cacgctgata ttcggcccag taaacttcgc ccattcgcgc
    22141 gtcaatggct gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt gcgccatcgt
    22201 cattagcgtg gagacgccaa tcatcggtag ctctgcgcca agtgccagcc cttgcgcaat
    22261 gccaatacca atgcgcacgc cggtaaagct accggggccg cgcccgtaag ccagagcgtt
    22321 aatatcagtc agggaagttc cgctggtggt caggatatcc tgcaccatcg gtaagattcg
    22381 ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg acagtaccgt cgttccacag
    22441 ggcgacagag caggcctctg tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc
    22501 ttagatcaat aaaaaggcgc gcatcatacc atactccgta acaaattacc tggaggatgg
    22561 tatcgcaagg aaacgaaccg cacgggcaat gtcacgagtg cgtggcgcgg gcggcagact
    22621 ggcgagaaac gtcgcgccgt aaggacgcat caccagccga ttgtcacaaa tcaccagcac
    22681 gccacgatcg tcggcgtcgc gaatcagtcg ccctaccccc tgtttgagag taatgacggc
    22741 atctggtagt tgcacttcat cgaacgggtc gccaccgcgc aaacgacaat cttccatgcg
    22801 cgcttttaac agtggatcat ccggcgaggt aaacggcaac ttgtcgataa tcaccaatga
    22861 caatgtatcg ccacgcacgt ccaccccttc ccagaaacta ctggttgcca caagaagcgc
    22921 gttaccggcg ctgacaaatt gctgcaacag ttgccctttg ctggtttccc cctgcaacaa
    22981 aacgggaagc gtcatggtag cgcggaactg ctcggccaga tcacgcatca tggcgtgcga
    23041 ggtacaaagc ataaaacaac gaccgttgtt agcttcgatg atcggtcgca gcattgccgc
    23101 taactggcga gcagaacctg gctggttggt ttgcggcaga ttgcgcggca cacagagtaa
    23161 cgcctggcgg ctgtaatcaa acgggcttgg caatagcaac gactccgcct gttctatccc
    23221 aagccgcgag gtgaaatgat gcagatcgtc gttcaccgac agcgttgctg aggtgaagat
    23281 ccagctaccg ggcttttgcg ccattaactc tttgaattta tccgccacgc tgagcggcgt
    23341 gagagccaga gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt
    23401 gatctctttt agccgcttca gccgtgtgcg atacaacgtg gcgcgctcaa atgccgcatc
    23461 cagcaaggcg gaacgcccca gtgacagttt cgccacgtca taacaaagtt ccagggtgtc
    23521 atcgagcagt aaaaatgccc gctgaatttg cggattagct aacagctcac gcaggttacc
    23581 gcgataacct ggctcaccga gttgcagacg aaaatcctgc gcactctggg caagacgatc
    23641 agcgcacttt tgtaactgct gggtgtcttt taattcggtg cggtaggcga tggtgatgtc
    23701 ttttgccagg tcgagcagtt gtcgactgga gagtgactga ccaaaatact ggctggcaat
    23761 gtccggtagc tggtgggctt cgtcgaagat catgacgtcc gcttccggga tcaactcgcc
    23821 aaatccactc tctttaacca ccatatccgc cagaaagaga tgatggttta ccaccaccac
    23881 atcggcgtcc atcgcttttt tacgtgcttt gaccacaaag caatctttat acatcgggca
    23941 gtcgctgcca agacagttgt cgttggtgct ggtgaccagc ggccacgcct gtgaatcttc
    24001 cgccacgctg acgcaggtgc tgatatcacc atcgactgtt tgattagacc aggagcgcag
    24061 caggatcaca tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg cctgctgttc
    24121 gagacgttca aggcagaggt agtttgagcg ccctttcagc agcgccacgt tgcccgtgta
    24181 tttcaacgcc tttgagactg ttggcaaatc gcggctgtag agctgatcct gcaacgcttt
    24241 tgagccggtc gagataatga ctttcttttt cgcccgcagc gcaggagcca ggtaagcgta
    24301 ggttttgccc gtaccggttc ctgcttccac caccagcggc tggccttttt ctatcgcctg
    24361 ggtgacggct accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt
    24421 cgccagctga ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg atttgaacag
    24481 ggattatgtc aggatgaggt cgctttcgcc agttgaagtg gtgacggcga cctcacattg
    24541 tggcagtctt tgcagcacga aaatggaagg ataatgagga caaaataatg actatcgttc
    24601 gtatcgatgc tgaagcccgc tggtctgatg tagtaatcca caacaacacg ctctactaca
    24661 ctggtgtacc ggaaaacctc gacgccgatg cctttgagca aaccgccaac acgctggcgc
    24721 agattgacgc cgtgctggaa aaacagggca gcaataaatc gagcattctg gatgccacca
    24781 ttttcctggc cgataaaaac gacttcgcgg cgatgaataa agcgtgggat gcctgggttg
    24841 tcgcgggtca tgcgccggtg cgctgcacgg tacaagcggg tttgatgaac ccgaagtata
    24901 aagttgaaat taagattgtg gctgcggtgt aagctttatc gaagcaaaat aagtcagacg
    24961 ataatttatc gataatactg gtcggtttta cataaatcga ccagagatta agat
//
LOCUS       DAPHDX010000065        25001 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPHDX010000065 DAPHDX010000000
VERSION     DAPHDX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.065"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..314
                     /locus_tag="RH058_002558"
     CDS             <1..314
                     /locus_tag="RH058_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3138132.1"
                     /translation="RNVGTGANQIPDMSSYASGSGWRKMPDGSIEQWGGIRFPGEHEP
                     VSANVSFPIPFTQTPNIIIVFDGGFGGGNMWGATNWSTTGFIAHCNYGLEGGVFFAKG
                     W"
     gene            314..757
                     /locus_tag="RH058_002559"
     CDS             314..757
                     /locus_tag="RH058_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3138133.1"
                     /translation="MMKYLYVNNLAYPYELQSLYVEKGEWPEEKGVDIDEVIFREYFY
                     DTPPEGKYRCVGEDGLPAWADIPPPTREEQIASAETKKQQLINQANEYINSKQWPGKA
                     SIGRLKGEELVQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(729..1331)
                     /locus_tag="RH058_002560"
     CDS             complement(729..1331)
                     /locus_tag="RH058_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3138134.1"
                     /translation="MEQAILGKDGYALNDGEIIVHNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSVDLNSWEYVPDHRGEVVYSTETGDTKEITVPGDYPEN
                     TTTIAPLTPYDKWDGEKWMTDTEAQHSAALDAAEAQRQSLINAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1332..2879)
                     /locus_tag="RH058_002561"
     CDS             complement(1332..2879)
                     /locus_tag="RH058_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3138135.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKALELK
                     VYADDQMAKHLAAPDPHSQYAPKASPTFTGTPKAPTPAAGNNTTQVATTAFVQAALTA
                     LINGAPATLDTLKEIAAAINNDPNFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAGMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLTYLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAPFSAEEYPKLA
                     KAYPKLKLPDLRGEFIRGWDDGRGIDSARLLLSSQAASILEHNHEMHGWTGNPLMARD
                     VGELGTSSVFAVQLSIGDGGILYSWKDGSGTTNDSKRMDRTNHVSSGAGDGSPRNIAF
                     NYIVRAA"
     gene            complement(2876..3481)
                     /locus_tag="RH058_002562"
     CDS             complement(2876..3481)
                     /locus_tag="RH058_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDV3138136.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(3474..4382)
                     /locus_tag="RH058_002563"
     CDS             complement(3474..4382)
                     /locus_tag="RH058_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDV3138137.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(4369..4728)
                     /locus_tag="RH058_002564"
     CDS             complement(4369..4728)
                     /locus_tag="RH058_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDV3138138.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(4725..5303)
                     /locus_tag="RH058_002565"
     CDS             complement(4725..5303)
                     /locus_tag="RH058_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDV3138139.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGELETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(5372..5818)
                     /locus_tag="RH058_002566"
     CDS             complement(5372..5818)
                     /locus_tag="RH058_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDV3138140.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSHMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTSASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(5811..6242)
                     /locus_tag="RH058_002567"
     CDS             complement(5811..6242)
                     /locus_tag="RH058_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3138141.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            complement(6338..6766)
                     /locus_tag="RH058_002568"
     CDS             complement(6338..6766)
                     /locus_tag="RH058_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HDV3138142.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSALLRQRQRRIEKLKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGAGSTAH"
     gene            complement(6763..7140)
                     /locus_tag="RH058_002569"
     CDS             complement(6763..7140)
                     /locus_tag="RH058_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138143.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALAALIQNGPLWQELRASLLKTITRKENVFISWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(7142..7654)
                     /locus_tag="RH058_002570"
     CDS             complement(7142..7654)
                     /locus_tag="RH058_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3138144.1"
                     /translation="MNPSIVKRCLVGTVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDSVVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(7635..7850)
                     /locus_tag="RH058_002571"
     CDS             complement(7635..7850)
                     /locus_tag="RH058_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138145.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(7854..8057)
                     /locus_tag="RH058_002572"
     CDS             complement(7854..8057)
                     /locus_tag="RH058_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDV3138146.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(8057..8521)
                     /locus_tag="RH058_002573"
     CDS             complement(8057..8521)
                     /locus_tag="RH058_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDV3138147.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(8617..9267)
                     /locus_tag="RH058_002574"
     CDS             complement(8617..9267)
                     /locus_tag="RH058_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDV3138148.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLATTGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(9271..10329)
                     /locus_tag="RH058_002575"
     CDS             complement(9271..10329)
                     /locus_tag="RH058_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDV3138149.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGMGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRNAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            complement(10346..11179)
                     /locus_tag="RH058_002576"
     CDS             complement(10346..11179)
                     /locus_tag="RH058_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDV3138150.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            11322..13088
                     /locus_tag="RH058_002577"
     CDS             11322..13088
                     /locus_tag="RH058_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDV3138151.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKSPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            13088..14125
                     /locus_tag="RH058_002578"
     CDS             13088..14125
                     /locus_tag="RH058_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV3138152.1"
                     /translation="MGKSKKNRAAATKQSQLKSQTTAEAFSFGDPIPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLVFVRNELMPLQKRLHELNEWVGAEVI
                     RFDDYVLGVQE"
     gene            complement(14172..14510)
                     /locus_tag="RH058_002579"
     CDS             complement(14172..14510)
                     /locus_tag="RH058_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HDV3138153.1"
                     /translation="MNKIAYKLPEGDLASRNQAIPQRHKIEGFIKEGYTVDLDLSGVY
                     SISESYSDEIFGVLVVKFGVTKVLSQVKVRNAPPSILKSIAKVIQRRSNEVASKKVHS
                     VGFDGLYAVC"
     gene            complement(14510..15484)
                     /locus_tag="RH058_002580"
     CDS             complement(14510..15484)
                     /locus_tag="RH058_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004205800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138154.1"
                     /translation="MGLGLKDAIVHTAAFHQKETNELQLPNNHWHPGFITVLAAYVNH
                     HKITEDNCVLSCPGYMRAINLHGALWGEDQYQQERVNVGRNYSLVTALTNVEAVDVAT
                     GSINSCVRQLTFPDRDPRDCPKGLTDLTHVIGELHDNVWSHGKSTGFSFAQRSAVPHT
                     QKKEHYLEFSLADCGLGFLRELRRAGIPGIETHQDAIAWCIQEGHSSKHADLQDDWAQ
                     QLPQDFIGGSVFGHGVSVKEKDNNHQGLGLYHLMKLIKTYNGELQLASGNVCLEAIGD
                     EVSYTELRTEWPGVAISCRFKIHELAVDKDSEENDPQLMEIMRALGGE"
     gene            16072..16950
                     /locus_tag="RH058_002581"
     CDS             16072..16950
                     /locus_tag="RH058_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138155.1"
                     /translation="MAMSLSCPLILLGLALFFYDWDRDMKKLTGVIAFALLLTACDKP
                     KIDASSDQSMKESIQKVRESLPADKKAQFDDAVKVVAFSQINMRELMQAGTSSGDVYE
                     TKIKSALEGKTGDEVINYAQTIRLEREKREKEQALQEIKELEAKQTSATQAAEKMKAF
                     KVERSRFYFQKEDYGNDQPILDISVENGTDKAVARVFFKGVIASPGRSVPWFSDVFNY
                     KISGGLEPGEKANWKLAPNRYSDWGKLKVPADAIFTVTVIGLEDADGKSIYGDAEFSE
                     RDADRLNQLRDKYLSK"
     gene            17059..17358
                     /locus_tag="RH058_002582"
     CDS             17059..17358
                     /locus_tag="RH058_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138156.1"
                     /translation="MKRITFSFTLVAIIIALAGCALPDKDGDFGAYVHACQQYAYGKS
                     YAFENRDFAYKVCKDAAKLWSEEVPGYIIRQIQLHPEIPSEEIKYAAMAGSLGNN"
     gene            complement(17426..17614)
                     /locus_tag="RH058_002583"
     CDS             complement(17426..17614)
                     /locus_tag="RH058_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138157.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYSRSMDVIS"
     gene            complement(17768..20182)
                     /locus_tag="RH058_002584"
     CDS             complement(17768..20182)
                     /locus_tag="RH058_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDV3138158.1"
                     /translation="MSHADMNNCSGFNEVAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYATDNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKMAHEQQR
                     FNPDMVILTDVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKQCLERLEHVRE
                     SQLSTSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWERVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQHEKDESADGDTRTDITCMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITEYQRQQLVYELKSRGFDGSEKEVDLLLCGGSIPSGAGLRIFYRNQRLQEDD
                     KWRNLY"
     gene            complement(20179..21036)
                     /locus_tag="RH058_002585"
     CDS             complement(20179..21036)
                     /locus_tag="RH058_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDV3138159.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PCYLVADINPDLINLYKKVAADCEVFISRARALFEDANREVTYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRSVD
                     SSAVYGVRA"
     gene            complement(21033..21260)
                     /locus_tag="RH058_002586"
     CDS             complement(21033..21260)
                     /locus_tag="RH058_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDV3138160.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(21260..21493)
                     /locus_tag="RH058_002587"
     CDS             complement(21260..21493)
                     /locus_tag="RH058_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDV3138161.1"
                     /translation="MRNIEILTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(21561..21902)
                     /locus_tag="RH058_002588"
     CDS             complement(21561..21902)
                     /locus_tag="RH058_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HDV3138162.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            complement(21866..22066)
                     /locus_tag="RH058_002589"
     CDS             complement(21866..22066)
                     /locus_tag="RH058_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HDV3138163.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            complement(22074..22583)
                     /locus_tag="RH058_002590"
     CDS             complement(22074..22583)
                     /locus_tag="RH058_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HDV3138164.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            complement(22616..22837)
                     /locus_tag="RH058_002591"
     CDS             complement(22616..22837)
                     /locus_tag="RH058_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDV3138165.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            22963..23532
                     /locus_tag="RH058_002592"
     CDS             22963..23532
                     /locus_tag="RH058_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HDV3138166.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALCEDGFYIFD
                     REFLPSAFKNLFVITDNNSEFICDKEFDDIRDGKWVISIDGEITIRDITRLPGGRIFV
                     EGGNRAFECKIEDVEIIGKIISLTIKYVR"
     gene            23548..23739
                     /locus_tag="RH058_002593"
     CDS             23548..23739
                     /locus_tag="RH058_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138167.1"
                     /translation="MLGKVFFVVLSCSLLLNPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            23926..24942
                     /locus_tag="RH058_002594"
     CDS             23926..24942
                     /locus_tag="RH058_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3138168.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVIELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAINLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLEV"
BASE COUNT         6045 a         6722 c         6083 g         6151 t
ORIGIN      
        1 aaaggaatgt ggggactggg gcgaatcaga tacctgatat gtcatcatat gcatcaggtt
       61 caggctggcg aaaaatgcca gatggttcaa ttgaacaatg gggaggaatt cgttttcctg
      121 gtgaacacga gcctgtatcc gccaatgttt cattcccaat tccatttaca cagacaccga
      181 acattataat tgtgttcgat ggtggtttcg ggggcgggaa tatgtggggg gcgaccaact
      241 ggagcactac cggcttcata gctcactgca attatggtct tgaaggtggt gtatttttcg
      301 ctaagggctg gtaatgatga aatatctgta cgtaaacaac ctggcatatc cttacgaact
      361 tcagtctctt tatgttgaaa aaggtgaatg gccggaagaa aaaggtgttg atatagacga
      421 agtaattttc agggaatatt tctatgatac accaccagaa ggaaaataca gatgtgtcgg
      481 agaggatgga ctgcctgcat gggcagatat tcctccacca acacgtgaag aacaaattgc
      541 atcagccgaa actaaaaagc agcaattgat taaccaggcc aacgaataca tcaacagtaa
      601 acaatggcca ggtaaagcat caattggtcg tctgaaaggt gaggaactgg tgcaatataa
      661 tttgtggctg gattatctgg acgcactgga actggtcgat acttccggtg cgccagatat
      721 tgaatggcct acgcctccgg cagttcaggc cagatgacgt ccggcgctgt gctggtatct
      781 gttgccgtca ccgcgtcaat gtaatccagc acagcgttaa gtcgggttgt ttctgcctgc
      841 gtcaacttcc gtccggcctg taatttcagc tgaatcagac taatggaagc cattgctgca
      901 ttaatcagtg actggcgctg tgcttctgcc gcgtctagtg cggcgctatg ctgtgcctcg
      961 gtatccgtca tccatttctc accatcccat ttatcgtatg gcgttaatgg ggcgatagtg
     1021 gttgtatttt cagggtaatc acccggaact gtgatttctt tggtatctcc cgtttcggta
     1081 ctatagacaa cttcaccgcg atggtctggc acatattccc atgaatttaa atctacagag
     1141 cgacagattg cataaccagc tttatgtgta cctggtgcat ccagacagga acatgccggg
     1201 ataccgacgc cgacagcgag atattcagtt gatgtagaaa tatattcccg cgtttcgcca
     1261 tcatagttat gtacgatgat ttccccatca tttaaagcgt aaccgtcttt tcccaaaata
     1321 gcctgttcca tttatgcagc cctcacaatg tagttaaatg caatgtttcg cgggctacca
     1381 tcaccggcgc cggaactgac atgattggtt ctatccattc gtttactgtc atttgttgtt
     1441 ccactcccat ccttccacga atacaatatc ccgccatcgc caatgctcaa ctgcaccgca
     1501 aatacggatg atgtacctaa ttcaccaaca tctctagcca tgagtgggtt ccctgtccat
     1561 ccatgcattt catggttatg ttcaaggata gatgctgcct gacttgaaag cagcagtctc
     1621 gcggaatcaa tacctcgccc atcatcccac ccgcgaataa actcaccgcg caaatccggt
     1681 aattttaact ttgggtaagc ctttgccagt tttgggtact cttcggcaga aaaaggtgca
     1741 ccgttgcatt tcagccagcc tgttggtggt gtggctgaag gccacggaac aggcacacca
     1801 acgggtaacg ctgagccttc tcccaaacca aggtatgtga gaagaccagc tacatccttt
     1861 ccactcaaat tggtaagcgt attgtccagc ggttgtttac ctgccagcgc attaagcatt
     1921 gtcgtggcaa agttcggatc attccccagt gccgccgcca gttcgttcag tgtatccagt
     1981 gcagcaggtg cagaacccac cattcctgca atcgccgatt tcacaaaagc cgtagtggca
     2041 atctgtgtat tgttgaccga ctgcgccgcc gtgggggctg ttggcgttcc ggtgagtgcc
     2101 ggactcgaca acggtgcttt tagtgccagc gcattgttaa tggtggtact gaaattcgga
     2161 tcattgttaa tggctgcggc tatttctttc agcgtgtcca gcgtggctgg cgcaccatta
     2221 ataagggccg tcagtgccgc ctgtacaaac gcagtggtcg caacctgcgt ggtattattc
     2281 cccgccgctg gcgttggcgc tttgggggtt ccggtaaatg tcgggctggc ttttggcgcg
     2341 tactgtgaat gcgggtccgg tgcggcaaga tgttttgcca tctgatcatc cgcgtacacc
     2401 ttcagctcca gtgccttgtc atccacatac ttgcgggttg ccagcactac agcagggtcg
     2461 attttcaggg tgatattgtc cgtgctgctg gtaatcagca ccatgcgcac ggtctgagtg
     2521 cgcccgctac cttcagccag ttgcggctta tagctttccg ggcagttgcc cacggcaatc
     2581 aatgcccctg actcatcaaa caggcccact tcacgtatcc accaaccgcc ctcgttttca
     2641 gggatcacct gttcggcaat aatctggctg ctgttctgcg ggtcgatata aagcatattc
     2701 agcgcagccc ggcgtttctc atttaccagt gccgtctgct ttgcgtccgg cgttggcaat
     2761 actccaccgc catcgcccac cgccatatgg gtaattttta gcggcacacc gagcgcggcg
     2821 gcgctggcaa gtttcgccgc gccaatatcc gtcagcaggg tataaaattt tgtgctcatg
     2881 gattcactct cattgtgtca ataacatgga ccgccccgcc ttcatgcgcg gtgccaccgg
     2941 aaataatcgt ttcgttgata tacggataga tcgtgatttc ttcgccaaga tagctggcgg
     3001 ctcccaccca atgcgggccg ctggtctgca gattgatgga catgccgatc atgtggcggc
     3061 tacatggttt ggcatcgctt atcagtcgct caagttccag ataggtatct tcagtgatgc
     3121 cctggtcctg cacgccgata tccaggcgaa acgtgcccgg tgtttctccg gtctgccacc
     3181 actcaataat gcggatcagg aatccgaacg gctccaccac ccgccgcacg gcactggtgg
     3241 tccctttatg ctgatgaata taaaaagcat ccttcaccac ctggcgcttg acgctttctg
     3301 tccagccctc gtcccagcga tccacagaga acgcccaggc gagataaggc aggaaactga
     3361 ccggacaggt tgccggattc cacaaatcac gaagcggcac ctgcagatca gaaatcccgc
     3421 tgcaggtttg cgccagtcgg cgctccagtg gtgttgaacc cggtggcagc agactattca
     3481 tccgttcctc cgttggttac gctccactgc gtacatgatg ccgcctgtgt tttgttcagg
     3541 accacatccg ccagaggaga agccagctcc acacgctgca ccccctcaac atgcagtgcg
     3601 gcaaagatgg cgctacggcg aatatcccga ccaagacgcg tctgactggc gatgtacttc
     3661 tgcaggctgg cttttgccgc tgccattacc ggctctgctt ccggtccagg atagagaaaa
     3721 atggtggctt ccacgcgata cgggatgatt tctgcgctgc gaaccgtaag acggtcagcc
     3781 accgggcgga cgttctcact gttcagagct ttttccacca cgtccagcag gtctttttct
     3841 gcagttccat cgccttcgcg gctaaggaca gtcagcacca cctctgcagg tgccgggctg
     3901 gttgcactgg catccgccac ccgaccgtcg gcgcttcggg catgaaattc ataagctgca
     3961 gttggccccg caacagaaag cccttcaaag gctgcaggca cacgcaggcg caacgcttca
     4021 tcgctttcca tcacagctgc aacgggcggc acagcatcat tatcagcagg cgtcaccgtc
     4081 aggcgtgtca cgttgtagtt ggcagcgagc tggtcaagat cgccgcccat cgcgtaagcc
     4141 accatcaccg cctgcgcggc ttcgttaatg cgctggcgca gaagcaactc acggtaagcg
     4201 ttctcctgca acaatttagt gacgggttca gattccagtt ccagcgtgcg gatcactgct
     4261 tcctgctcat ctttcggatg aagcgccaca aattctgcct tgcgttcggc aagcagcgtc
     4321 tcaaagtccg gcacatccac aatctgcggt gcaggcaact gcgaaaggtc aatcactgcc
     4381 attctctgct cctgttgata cggaaaggga cacaggcaca ccgttattcc gccgcccggt
     4441 cagctccacc accattgaac cgtcaaaatt gctgttgatg gtgatggaat ccagcgtcaa
     4501 ccgtggctcc cagcgactca gcgccacata cactgccgac atgacctgca ggcgtaatgc
     4561 cggattttgt ggctgatcta tcagtgccga cagcagggaa ccatattccc ggcgggcaat
     4621 acggctaccc tgcggtgtca gcagaatgtc ccgcaccgac tggcgcagat gatcaatatc
     4681 agtaatgact ttgccgctgg tattgttcat cccgctataa agcgtcatac cgggcctccg
     4741 gttgtatcgc cgcctttcag gacgccagta tgctgatgcg catcaaccac gatcccgtta
     4801 gaactcatcg ctccgccgcc ctgggtaacg ccaccattga tcaccacttc gctgttaatg
     4861 cgcgtgcggt cagcctccag tacaaactca ctggttttca tggtgatgtt gtcagcagcc
     4921 tcaatgacca ttgatttgat gcccctgaca taccagcgcc cggtggcggg ttcgtattca
     4981 aaccagccac cgtcaggatg ttctgtcacg caggcgtccg ccgacgtcga cggtggtgcg
     5041 aactgattcg aatagacagc gggcagcgca aaggcggttt ccagattgcc gcccagactc
     5101 agcagcacca cctgctcacc ttccgatggt cgccaccatg tacgggcatt cccggcacgc
     5161 agcgtcagcc agctgatcca gttggtttca agctcgcccg ttttcacccg gcaaagccag
     5221 ttttccctgt ccacttcggt gactacccct gtgcggatca ggttggtgat aaggcgcatg
     5281 atttcggtta attgtgcgtt catagggaaa ggttgccatc aggggaagaa aggcggcagt
     5341 gctgcaactt gtatcagtgc tgatacaaag atcaccccgc cagccattgc agaatcatgt
     5401 cgcgggtcat tgcctcaaca tcatcattta cacccagaag gcgacgctct gcgtaacgca
     5461 cctccggtcc cttacgactg acgcgatctc gcaggccgta atggtgaacg cgggcaatgc
     5521 gctgcacctt gccttcaaac tgcacgctgg cagagtcggc gctggcggaa gttttcaggt
     5581 attttgtggt gcgcagcttt gcaaacatct gacgtttgat acggcctttt ttactgcgtg
     5641 ctgttacccg tcgcggttca taactgctgc catctggatt gcgctgcatt ctgatatttt
     5701 gctgctgtgt ccggcgtagt tcctgcgcca gctggcgcat catgtggctt cttgcggctg
     5761 gctccagatt cgccagcaag gcactcagcc agtcgtccac tttctgcagt tcagccacgt
     5821 ttcaccgtcc acatttcttc aggttcatca ggttccgcta tagcttcaac gctcgacaca
     5881 ctgccgtcag tgctgaccag cacacgctcc gtcagttgca ggttgaggct gatatcacag
     5941 acatcgttgc gcagaatatc cacctcaaag gtgaatagct tttcccgtaa cgccgggtta
     6001 ttgatggcat cgggctggtt atcacgcagc cacagcaaaa ccggggccat cagcagattc
     6061 tggtcgccgc tgaaatcctc aatcaccacg ttcagggtgt agcggtactc ccatgacatg
     6121 gagctggccc ccgtggcaac cagcgaaccg ttatccacaa acagatgcag tttgtccggg
     6181 ttattgcgaa cataaggcac cgctttattg agggcgtggc gcagggattg tggtttgttc
     6241 actgtttcgc tcctgacacg caataatcat gtccactttg tctgcacaga ccgcccaggc
     6301 ggcctccgtt tcatccagca acgcattcag atcaccgtta gtgcgcggtg ctgcctgctc
     6361 cagccgacac ggcgtcactc gcggacaacc actgacggta agctgcacct ccggtgagcg
     6421 tcggacgttc ccgcagccgg ataatgtcag caggcaaagg agtatcagcc cagcggcgta
     6481 aatcctcgtt ctcacgtttc agtttctcaa tccggcgttg tcgttgtctc agcagtgcgc
     6541 tggtctgttc tgcttcggca tagagccgcg cctgctcccg gttattggtt tcagtcagaa
     6601 tggacaggct gataagctgg ctgttgctct ttgccagtgc ctggcttttg ctctgcagct
     6661 cgtctgcctg cgtgctaatg gtctggctgg catcagccag ccgccacgtc tgccagccca
     6721 gagccgccag taataacgcc agcacaacca gcagcaaccg gttcatgctg ctacctgttg
     6781 cgccatctga ttacgggtga tccagaaggc aataacggtc agtagataaa agaccagggt
     6841 aatagcccac cccgtccatg cgagacttac aacaatcagc aatcgcatca cccaactgat
     6901 aaatacgttt tcttttcggg taattgtctt cagcaaagat gcccttaact cctgccagag
     6961 cgggccattc tgaattaacg cagccagtgc taccggaatt accgcccatg tcagcaaaca
     7021 ggctacccaa acgccggacg ctgccagtac cggaaagatc ccctgcggat acaccattgc
     7081 tgcgattaac agcgccatcc ataacatcag aaacagtccg ctgattaatt tctttttcat
     7141 ttcagtttgc tccctgtaaa caccaggcca tctcccgcgc acggcggtta tccagcccct
     7201 gattaaacac accttttaca taaacccagc gcggcaactg tcggcacgca tccgcccagc
     7261 gccgctgatt gagcaatttc accagcgtgg agctgcaggc attgcctgtc cccacgttga
     7321 aggcaaacga caccaccgag tcatacacct tttgtggcgg ctgttgcttc acacatcttt
     7381 ccagcgcccg ctccacacgc agcacgttgg agataagccc ttctgctgcc tgtcgttccg
     7441 taatggtttt gccgggaatg acgcccgacg tattaccaat gccgtcagtc catacacccg
     7501 cgctgcactg atacggctgc agacgacagc cttcgtaatc ggcaatcagt ttcagtccct
     7561 ccacggaggt gtgaagctgc tgaaaacccg gcagcgtggc agcaatagcc agcacggtcc
     7621 cgacaaggca gcgtttaacg attgatggat tcatagtcct cccgcgagat ctgcccgtcg
     7681 cgcagaagct ggtaggcttt gtgtttgtag taccagttga tagccagcat cagcacacca
     7741 atcatcaggc cgcccagcgt tgaggcatcc ttgatggaca aatcgcccag ccaggccagc
     7801 acaacggcga tgcagtacgt gataaaggcg ctgattcgct caagcgtcat aattcagtcc
     7861 catagctgga cggtctgcac ggtggtggtt gtcggaatgt ccggcagctc cacctgcagc
     7921 ccgtgaggta aaaaggggcc atattcggca agccccggat ttgccttcag tacctgctcc
     7981 gtgacaccct gcgtgcgccc gtaatgacgc cagcaaagcg cgtccaccgt gtcatactga
     8041 tgcgcacgca ctttcatcag ataagctcca ctgtgcagtg cggcgcgtcc tgtacccggc
     8101 tgatggccca gcgggcgtca cgccataaat caccgctggc ttccgccagt tcctcgcccc
     8161 gcttcacacc ggatgccgtg gcgtcatagt cctggtaacg ttcgttgagc atggcgcgtg
     8221 cccagcagta aaccgcgttg aaatagtgct gaatgcgctc acttttgccg tccagctgtt
     8281 ccgccggaac ctctgccagc gaggcatacc ccagcatctg ctggcgtctg cgaaactcat
     8341 acagctctgc gttgacctcc gaaattgccg acagcgcaac ctgctttaaa cgcggctgcg
     8401 tcaccgtgcc gtcagtgcgc atgacactgc gaaactccga caggtccaca tcaggccaga
     8461 acggcgtatt tctgatgatt tccgcctgtt ccggtgcctg ttctggcgca acaaacttca
     8521 tgctgctttc tcctgaaata gagggcggtg gacggggttt tgatgtggca gtgcctttcg
     8581 ccaccccgtg ccgcccgtgc gcgggggcac gttctgtcag cggctgtcat tgcgcagtct
     8641 gcgctccagc tgctgtttgt cttttttcac gccacagcgg ggatcgagct gtaacgcatg
     8701 gttgagatga ttaagggcag acgccggatt gctttcactc aggaccgcgc caatcgcttt
     8761 atgcagacgc gcccgtgact ggtccggcat atccagaccg tctgtcagct ccagcgtctg
     8821 cagcaacaga tcggcatcaa agccggtggt ggcaagcatt gcgctctgcg cggcgtctgc
     8881 catttcctct gccagcacgg tctgcacgtt gcggttaccc agcggcatca cccagccatg
     8941 acgcagggca tgacgcccga tctccagcgc tccggcataa tctccggcat caatgcgcca
     9001 cagcatcacg tacatcagca cgtcatcctg ttgagcgcct ccggcagcca ggacaccctc
     9061 tgcccaggcg gcgtacttcg gcagcagctc caccttgatt tccgcttttt tgaccgtgga
     9121 ctgaacgccc ttgagacggc ggcggtcttc cgccagttgc agcagcatca ggtcatagcc
     9181 cgatgcgtgg cgaacactgc cgccctcgcg ggcggcctgt tcagcctgaa cgcgcaggcg
     9241 atgctgccgt gcgggactca ggctcatgga ttacgctccg gtttcggctg cggcggcgct
     9301 gaaatcacca atctggatgt tttccaccag tgcggcgcag cggtagtcct caaccacata
     9361 ggcttcatta acggattcaa agttttcaat ccggtcacgt ttcgggttgt cgataaccga
     9421 acgacggcgg gtgtcttcct gccagtagat ggacaggtta tccagacggg tgatcagcag
     9481 cgcattcggc gggaagaacg gcgcacgcac ggcctgcagg ccacccatgc gtttctgact
     9541 gatgatcata tcggcagcca gtttttcact gttttcctgc tctttgttga ccagcgggaa
     9601 atacttgtca gacagcagtt cacgaccgca aatcacaacc agatcgtcat cgtcctgata
     9661 gaccacgtcg ataagctcgt taacggcatc catcaccacg gcgtccaggt tggcatattc
     9721 gccacctttc ccgactttca ccgcacccgg tgtggtttca ccgcccgtgg tggtgctgcc
     9781 catgacgtga tccggtgcat cctcacggat tttctgcagc cagcctttat tcacatcctg
     9841 cagtagcggg ttttcgctac ggttggaggt tttcgcacgc ttcacgccgt taaagccgat
     9901 catgatgcgg tccagtgcct gacgtttcac gatggcgtta cggatacgca cctggaaatc
     9961 ctgaaacttc gcccacaggt ccagcttcgc gtaggtcagc acggtgtcaa agttggtctg
    10021 ttcgcatttg tattccacat cgaccatcag cgtcggatcg acaggttcac gctctttcgc
    10081 ggtggtgtca gtggttccgg caatggtgct gccaacaccc atccccagca gctgaccgga
    10141 ctgctcagtc actggcgtga cgttaatcag cgtcaggaaa gcggcggact gctggatctg
    10201 gtcttccagc gtctgctgca cagacggctc tacagtgaac ttgctggaca gttcttcaac
    10261 tgccacaccg ttcagacgcg ccagctgctg caggtaagcg ttaaaagcaa agcgggtatt
    10321 cttcttcatc gggttttgtg ctccatcagc aattggtcag agtgtcagcg ggggcgttac
    10381 cgcctgttgc acgctggcgg tagtcctggc ggctgtcttc atgactcagc ttgtccacca
    10441 gttcgttaaa ggcggtttgc tgtgcctgca gggcagtctc cagctcagac aggcgttctt
    10501 cctgctcaga cagggatttt tcggtgcgtg cgctcaggtt ctgctgctca gtggcgacca
    10561 gctccacggc cttatgcaca tcagagaacc gggcgtcatc ggactgctct tttttggtaa
    10621 acagcgccgt gacgcgggca aacagggacg gtttgtcgtc ctggatttct tccagttcga
    10681 tcaccgtttc ctctgcagcg gtaaagagat tggcaggatt ctgcttgcgg tttgccagcg
    10741 ggttatgggc tgcactggcg ctgaatgtca gcatttccgt acccagactg gcgggatcat
    10801 cagtggcagc caggccgacc aggtaggctt tgcccgtatc agcaaacttc gggctgactt
    10861 ccatagaggt gaataatttc tgtccttttt tcaccagttc caccagggac tccgttggct
    10921 caacgtcggc atacagcgcc atcttgcctg ccagcgggcc ttccgtgatt tcttcagcaa
    10981 acagcgccgt caccttgccg tagcggttaa aggtgctgtc cggcagataa gacttgatgt
    11041 gctcaaggtt aatcagcgcg gtatacaccg ccgggttgta gctggctgcc atctgttcca
    11101 gccattcacg ctggatttcg cgtccgtcgg tggtggcacc ttccaccccg atgcgaaaac
    11161 gctttgcttt cactgtcatg agccgtgctc cgttagaaaa aacttactgg agccttatgg
    11221 ttgcggtgat gggggcagtg aaacaatgcg cggtatttgt accgacaacc acacaaaccg
    11281 caggcgggga aagccttcat tcaaggctgt aggtttgtgc catgaacacc acactgacac
    11341 ccgcagatct cgatccccgt cggcaggcca tgctgctgta ctttcaggga taccgcgtag
    11401 cccgcattgc tgaaatgctg ggcgagaaag ttgcaaccgt tcacagctgg aaaaaacgcg
    11461 acaagtgggg tgattatggg ccgctggatc agatgcagct caccaccgcc gcacgctact
    11521 gccagctcat catgaaggag cacaaagaag ggaaagattt caaagagatt gacctgctgg
    11581 cgcgccagtc ggaacgccac gcgcggatcg gcaagtttaa caatggcggc aacgaagccg
    11641 acttaaaccc taacgtcgcc aaccgcaaca aaagcccgcg ccgtcagccg gaaaagaacg
    11701 ttttcaccga tgaacagatt gagaagctgg aagaaatttt ccattcctcc atgttcaact
    11761 accagcgcca ctggtgggaa gccggaaaaa ccaaccgcat ccgcaacctg ctgaagtcac
    11821 gccagatcgg cgcgaccttt tactttgccc gtgaagccct gattgacgcc ctgcttaccg
    11881 gacgtaacca gattttcctt tccgccagta aggcacaggc ccacgtcttt aaacagtaca
    11941 tcatcgactt cgccaaagaa gtggaggtgg agctgaaagg cgatccgatg gtgcttccca
    12001 acggggccac gctttacttc ctcggcacca atgcccgcac tgcccagagt tatcacggca
    12061 acctgtatct ggatgaatat ttctggatac caaaattcca ggagctgcgc aaagtggctt
    12121 ccggtatggc tattcacaaa aaatggcgac aaacctattt ttccacgcca tccagcctga
    12181 cacacagtgc ttatccgttc tggtccggtg cgctgttcaa ccgtgggcgc aacaaagccg
    12241 ataaggtgga catcgacctg tcccacagca atctggcccc cggcctgctg tgcgcagacg
    12301 ggcaataccg ccagatagtc accgtggaag atgcggtgcg cggcggctgt aacctgttcg
    12361 accttgacca gttgcgcatg gagtacagcc cggacgaata ccagaacctg ctgatgtgcg
    12421 agttcgtgga cgatctcgcg tccgtgttcc cgctcagcga gctgcaggcg tgcatggtgg
    12481 acagctggga agtctggacc gactttcatg cactggctct gcgccccttt ggctggcgcg
    12541 aagtgtggat cggttatgac ccggcaaaag gtacgcaaaa cggcgacagc gccggatgcg
    12601 tagtggtggc accgccagcc gtgccgggcg gtaagttccg cattcttgag cgtcaccagt
    12661 ggcgcgggat ggacttccgc gcccaggctg acgccatcaa aaaactgacc gaacagtaca
    12721 acgtgacata catcggcatc gactcaactg gcgtcggcca cggggtttac gagaacgtga
    12781 aagcattctt tcctgccgtc cgggagtttg tctacaaccc caacgttaaa aacgccctgg
    12841 tactcaaggc ctacgacatt atcagtcacc gccgcctaga atttgacgcc ggacacaccg
    12901 acattgcgca gtcattcatg gcaatccgtc gcgccaccac cgccagcggc aaccgcccga
    12961 cctatgaagc cagccgcagc gaagaagcca gccatgccga tctggcctgg gcaacaatgc
    13021 acgcactctt taacgaaccg ctgcagggcg agtccgccaa taccagcaat attgtggaga
    13081 ttttttgatg ggaaagagta agaagaaccg cgctgcggcg acgaaacaga gccagcttaa
    13141 aagccagact acagccgaag cattcagctt cggtgatccc attccagtac ttgaccgccg
    13201 cgaactgctg gactatgtgg aatgcgtaca gacagatcgc tggtatgagc ctcctgtcag
    13261 ctttgacgga ctggcgcgca ccttccgcgc tgctgtgcat cacagttccc cgattgcagt
    13321 aaagtgcaac attctgacca gtacctacat ccctcacccg ctgctcagcc agcaggcttt
    13381 ttcacgtttt gtgcaggact atctggtatt tggtaacgcc tacctggaga aacgcacgaa
    13441 ccgcttcggt gaagttatcg cccttgaacc ggccctggca aaatacaccc gacgcgggtt
    13501 agacctggat acctactggt ttgtgcaata cggtatgaca acacagccgt atcagttcac
    13561 gaaaggcagc atctttcatc tgatggaacc ggacattaac caggagatct acggcctgcc
    13621 cggctatctt tctgccattc cgtcagccct gctcaatgag tccgccacgc tgttccgccg
    13681 taagtattac atcaacggca gccatgcagg cttcatcatg tacatgaccg acgccgcgca
    13741 gaatcaggag gatgtgaaca atctccgcaa cgcgatgaaa agcgccaaag gtccaggcaa
    13801 cttccgcaac ctgtttatgt actcgcctaa cggcaaaaag gacggccttc agatcatccc
    13861 tttgtcagaa gttgcggcga aggatgagtt tctgaatatc aaaaatgtca gccgcgatga
    13921 catgatggca gcacatcgcg ttccgccaca gatgatgggg attatgccaa ataatgttgg
    13981 gggttttggg gatgtggaga aggctagtct tgtatttgtt cgcaatgaat tgatgccact
    14041 acagaagcga ctacatgaac taaacgaatg ggtgggtgca gaagtaatac gttttgatga
    14101 ctatgtttta ggtgttcagg aataaaagaa aaggcgctaa tctcagcgcc ttttatctaa
    14161 ctaaccatat attaacaaac ggcgtataaa ccatcaaatc caacggaatg aacctttttt
    14221 gatgctactt cattactacg gcgttggatc acttttgcaa tactttttaa aattgaaggg
    14281 ggtgcattgc gaaccttaac ttgactcaaa actttggtta caccgaattt cactaccaaa
    14341 acaccaaaga tttcatcaga gtatgattct gaaattgaat aaacaccgct cagatctaaa
    14401 tctacggtat acccttcttt aatgaaccct tcaatcttgt gtcgttgggg gatagcctga
    14461 ttgcgcgaag ccaggtcacc ctcgggtaac ttgtatgcga ttttgttcat tactctcctc
    14521 ctaacgcccg cattatttcc ataagctgag ggtcattttc ttcgctatct ttatcgactg
    14581 ccagttcatg aatcttaaaa cggcaagaaa ttgctacacc cggccattca gtacgtaatt
    14641 cagtatagct cacttcatca cctattgctt ctaagcatac atttcctgaa gccagttgca
    14701 attccccatt atatgttttt atcaatttca tcaagtgata caacccaagc ccttgatgat
    14761 tattatcttt ttctttgacc gaaaccccgt ggccaaacac actaccccca ataaagtcct
    14821 ggggaagctg ctgtgcccaa tcatcctgca aatcagcatg cttagatgaa tgtccctctt
    14881 gaatacacca tgcgatcgca tcctggtgag tctcaattcc gggtatacca gctcgccgca
    14941 attctcggag aaatcctaac ccacagtccg ccaaagaaaa ttctaaatag tgctcttttt
    15001 tttgagtgtg gggtacagcc gaacgttgtg caaaagaaaa acccgttgat tttccatgcg
    15061 accaaacgtt atcgtgaagc tcacctataa catgggttag atctgtaagt cccttgggac
    15121 aatctctcgg atcacgatca gggaatgtta actgccttac acaactgtta atactaccag
    15181 tggcaacgtc aacagcttca acattggtta atgctgtaac caaactgtaa tttcttccaa
    15241 catttacacg ttcctgttga tactgatcct ctccccataa agcaccatgt aaattgatag
    15301 ctcgcatata gccagggcat gacaaaacac agttatcttc ggtaattttg tgatgattca
    15361 cgtaagctgc taagacagtg ataaaaccag gatgccaatg attgtttggc aattgcaatt
    15421 cattagtttc cttttgatga aacgcagcgg tatgcacaat agcgtctttt aatcctaacc
    15481 ccatgaaccg aacctttttt atgcattttt cttatcatat aacttaggac tgatcagcgc
    15541 aaagctcccg acactattga atcatactaa attaaggcct ataccacaca gcacagcgcg
    15601 cgctcgtatc cccgccacgc ctgcccgctt tatgtagtag ttttcatgca cctgcatgac
    15661 atgagcaaaa gctcgccagt tctggcatgt cttagccaaa ccgatcctca aacgatcatg
    15721 cgaactcatg cagcatagac atgcatgaca tgaaaatcgg tcaaaccttg aaaccagtca
    15781 taaatatgag ccagcttaat acacactaaa tagctgcgtc taatactgct acacctgccg
    15841 tttactgaca tgtggcatgt cgcgtaagga caacatactg taggccagat acagcaggat
    15901 caggcgaaga aaacggcccc acgccttacc cagcaagggc caagggctaa ccgaaagtgg
    15961 agcattattc cgcgcagtta tttagtgtca gctcttttga gctaaaatca agtttcaaca
    16021 tgagggtctg tgcaggcttt ctcatcccgc taatttatac agcacaccta tatggctatg
    16081 agtttgtcat gcccgctaat cctgttagga ttagccttgt ttttttacga ttgggatagg
    16141 gatatgaaga aattaactgg cgtaattgct tttgctctgc tgcttactgc ttgtgacaaa
    16201 ccgaagatcg atgcatcaag cgatcagtcg atgaaagagt ccattcaaaa agtaagagag
    16261 tccttacctg cagataaaaa agctcagttt gatgatgcag ttaaagtcgt tgcctttagc
    16321 caaatcaaca tgagagagct aatgcaggct ggaacgtctt ctggggacgt ctacgaaaca
    16381 aaaattaagt cagcgctgga aggtaaaact ggtgatgagg ttataaatta cgcacaaact
    16441 attcgccttg agcgtgagaa gagagagaaa gaacaggctc tgcaagaaat aaaagaactg
    16501 gaagccaaac aaacctctgc aacacaggct gcggaaaaaa tgaaagcctt caaggtcgaa
    16561 cgttcccgtt tttatttcca gaaagaggat tacggcaatg atcagccaat tctggatatt
    16621 agcgttgaaa acggaacaga caaagcggtt gccagagtat tcttcaaagg agttattgcc
    16681 agcccaggaa gaagcgtccc ttggttctct gacgtcttca actataaaat ttctggaggt
    16741 ctggaaccag gtgagaaagc caactggaaa ttagccccta atagatactc tgattgggga
    16801 aaacttaaag ttccggctga tgctatattt accgtcacag tcattggcct tgaagacgca
    16861 gatgggaaat cgatctatgg tgacgccgag ttttcagaac gcgatgctga cagactgaat
    16921 caacttagag ataagtacct ttctaaataa tcaaaattaa agctacttca gcatagtccc
    16981 tgagaatata tatcagggac taccaaatca cggcagatac cccttagtta aataatgcta
    17041 aacgcttaga aagagaccat gaaacgtata actttctcct ttacgttagt agcaatcatc
    17101 atagccttag ctggatgtgc tttgccagat aaagacggcg acttcggagc ttatgttcat
    17161 gcctgtcagc aatatgcata cggaaaatcc tatgcctttg agaatagaga cttcgcgtat
    17221 aaggtctgta aggacgcggc gaaactgtgg agtgaagaag ttcctggcta tatcatcagg
    17281 caaattcaac tacatcctga aatacccagc gaagaaatta aatatgcagc catggccggc
    17341 tctttgggta acaactaaat caacttatga gaaaggggct taatgcccct tttcggttcc
    17401 cttatcaagt cctaacgaaa caagctcaac tgattacatc catcgaccta ctgtattcat
    17461 ggcttcgaat tttcgccatc aactcatcag tcaattcaga aacccactgc agagctagcc
    17521 ccttctcttc atcactacac tcactagccg ctacaagctt aagaaaaaaa tcaatgcgct
    17581 ggagcttcaa agactccaaa aaatagtcct gcatctttcc tcctataaca ccatacgcaa
    17641 tactgtacat ataaccactg tttatattta cagtatataa taatcttact gatgtaaaac
    17701 gtttttttac gttcatcagc ctgatatgcc tggtattatt aagagcacga attgttaacc
    17761 cgcgtgatta atacaggttc cgccacttat catcttcctg caaacgctgg ttccgataga
    17821 agatacgcag gcctgctcct gacggaatac tgccaccgca aaggagcaaa tcgacctctt
    17881 tctcgctgcc atcaaatcct ctggacttca gttcatagac gagctgctga cgctgatact
    17941 ctgtaattcg ctgtttgtag tctttacgcc gtttcggttt aaccaggcgc aatcttgctg
    18001 ccagttcccg gcgctctttt ttgctcatac tgtgcaggta atcgtgcaat tccttgtcat
    18061 ccatgcaggt aatgtccgtt ctggtatccc catctgctga ttcatctttc tcatgttggt
    18121 tcaaattttc agcaagggga cagttattgc cacgagtcca aggggcgcaa gcgccctggt
    18181 cggctgccgc ctcctgaaca tcaacggcct tacgaaccat tttccacttc acggcatgag
    18241 tgcagatctt gccctctgca ataggtgacc agatgccata aatacgaatg ccgtgatcgc
    18301 cataggcggt cggctcttcg ttgatttcat aagcggttct gatgaggtga tatttgcggg
    18361 gaaccagtac gccgccctgc ttcatgatgt aggtggcaaa acaaccagca tcagcagcag
    18421 ccaggatggc atcaaggcgc gggttatcca gtaccggcgc acctgctttt ttgtccccct
    18481 gttgccttgc cgcctgacca gccagcaatc gcagttcacg gtaagcctga cgccccggaa
    18541 tgccaaagaa gcggaattgc tgaacacgat gcagagacgc ccaggcatta acgtattcag
    18601 cattatcacg cagggatttc cccgtttcct tgctgatctc gccagccaga ccacgcccgt
    18661 caatgttctt actgatgtat ttcgcgatgt agcttgttgg cgtacctttg cgcgggttaa
    18721 tcaactcaga cttaaagcgc ggcccagtgt tattgcccag ttcctcgcgg tcttcacgga
    18781 tggcaaactt acgcagtaat gcagtgatgg cgcggcggtc ttttttgcgc ataaaacaca
    18841 acaggtgcca gtgaactgta ccgtcatgat gcggctcagc cacccgcacg ccataccacc
    18901 gcaatccggc tttgtgcatc gccttacgaa atgcagcaaa catgccgacc agataatcac
    18961 tgctttgtct taccgtcgca tttgtccaag ttgggttggg cctgccatta tttagcgtgg
    19021 aatggaaacg tgacggacag gtgatggtgt agaaaacggc gcagtcaccg cgcatttccg
    19081 cgataagctc caggccttta acacaggcca tcatctcatt gcggcgatgc gcagggttgc
    19141 tgctgctggc gtttaccaca tcctccatgt ccagcgtgtc gccgtcttcg ttcaccagtt
    19201 catgagaacg aaaaaactcc agcgacttac ggcgctgctc acgtttatgc atcacggctt
    19261 catagctgac ataaggagat gcttttttgc taaccaggca gacagcgcgc aactgctctt
    19321 cccgccattc gcaacgcatc ttccataatt tccgatacca ccagtcggcg cacaacatac
    19381 gcgccagcga acccggaatg agttcatagg gcacgggttt acggcggttt cttttccggc
    19441 ggagttgctc aaacgcagga ggaatgacat ccagacgcag ggtttccgcc gccacccttt
    19501 cccatgtctt gcggatttct tctggcttaa cgtcatcggt ggcatacaaa tcgccacaag
    19561 ctgcatcaag acacatgctc atatgcgcag caacaagggt ggacaggcgt ttcacctgat
    19621 cctgactcat ttcaggcagg atcagcaggc cgtccagccc ttcatggctt gccataaagc
    19681 gaaaagaagt ggatagctga ctctcgcgta catgctctag tcgttccaga cattgcttaa
    19741 tcgtctcacg taaatagcgg gaataagcct ttggcctgcc caggctgctg aaatattcaa
    19801 tacgttgcat cagcggcttg ctgatatggg aaggctgggc gttgacgtca gtcagaatga
    19861 ccatgtctgg attaaaacgc tgctgctcat gcgccatctt tgcccggcta atgagcttat
    19921 cctgctccat ttcgcgttgg acaggatcac gggattcatt aaagaaataa cgctcccaga
    19981 cctgatcact cagtgcctcg cggcgcagtt gttcctgctc gttatcggta gcgtacagag
    20041 tgatcaggtt tgaaagcgca gaaaccggcg caacttccgc cgggtccaga taagggttaa
    20101 tggccttttt cgggctgttc catgagaatg ctgcggcgac ctcgttaaag ccgctgcagt
    20161 tgttcatatc agcatggctc atgcacgcac tccgtacacg gcagaactat ccacgctacg
    20221 cgaaggatca aatcccaccc agcagcgcgg cccggaaaca gcgatgattt ctgttgcaga
    20281 tttactctca ccagctgcta caccgatgct gcgttttgct ttgatgtagt ggtgagtaaa
    20341 attgcgatac agcgaacgga tcagggatgt atcactgtta gaaacaatga ccggatgtcc
    20401 ttctgatgac cgatgttcaa gaacggatgc caggtgatac tggtcatctt cagtgaagcc
    20461 gtcagtgtga tagccggaaa acgtaccgtc ataaggcgga tcacaataca ccacatcccc
    20521 cacctgcagc atcgccagcg tttcatcaaa gctggcgcag ataaacgttg ctcgctgggc
    20581 tttctctgca aatgcgcgaa tttctttttc agggaaatac ggatttttat aattaccgta
    20641 tggaatgttg aaatgcccgc tcttgttata acgacataac ccacggtaac cgtgacgatt
    20701 gagatacagg aaatataccg ctttcatgaa atcagtaatt tcagttgagt aattaaactc
    20761 ctgtcttatg ttgtaataag tcacctccct gtttgcgtcc tcaaataaag ctctggcacg
    20821 agatataaac acctcacaat cagcggcaac ctttttatag aggttgatta aatcaggatt
    20881 aatatccgca accagatagc agggataatc cgtctccatc atcacagcac aggaacccgc
    20941 gaaaggttca accagtcgcg ggccagcagg aaggtgtttt ttcagttcgg acataatggc
    21001 ggttttattt cccgcccatt tcaggatggt gctcatacag cacctccgtt gtaatgtttg
    21061 cctttcagct ctgcaatttc ctggcaggta atgcaaagct gcacacccgg aatggcacgg
    21121 cggcgtgctg gcggaattgg cgcttcacac tcaatgcaaa gcacgcgaga cacgcccggt
    21181 gttttggcac gggcagcacg gatatggcgc tggcgttctt cttcaacgcg ctgctgtacg
    21241 agatccattg catcagccat tagtggatct cctgcgcttc gttctggatt gcttcagcag
    21301 tcacacgcag cagttctgcc gcttccacgt ggtttagctg acgggatgag atatgacacg
    21361 ccaggctatc aaggcgagct gccattgctt cagcccttgc ccggcgttct tccagacgag
    21421 cctctgtcag taaaatatta agccctgcat catccggtcc ggttttagtc gtgaggattt
    21481 caatattacg cataatcaat tctcctgaat ttagataaag ggatgctcgg cgggtttacg
    21541 ccattaattt cattagttgg ttaattcggc atggttagcc gtctgggaaa taagctcacc
    21601 actgcacgaa aatgattcat tgctttaatc aactcccgct tttcgtcagt ggtcagctca
    21661 ttgatgctga tgctatgacg ttcagccgga atttttgcca taaagaatat ggctgccagt
    21721 gctcgtttat tttgttcgtt attgatatcc cgtggatcac gcatatcttt aataaaccgc
    21781 tcaagctctg actcaatatt caggccaaaa acttttgccc ttaattccgc tatgtgatta
    21841 agtccattca ggcgttcacc ggggcttaat ggaacagtcg ccgcagcgcc ttcaatagcc
    21901 atttgttccc ccgttttttc gtagatagtt ctgccagcaa ttcatcttgt gaacggcacg
    21961 gatgccagcg ttttccatcc tcacccatga tccagccgtg accgtagtgc attgccgggc
    22021 tttgctttac cagcagcgat gcaaatgatg gttctttcgt cagcataagc acctcacagc
    22081 aaaccgaatg aagcaccgag gccagtcacg gtatcaactg cactcgccat cgcagggtta
    22141 gcctgtaaac gggcctgcaa tgaaacagca gccaacgcca tcagtcgtgt tacagagtta
    22201 atgctgctga tagcatcacg acgacctgca ctggttttta catcgccaga taccgcacct
    22261 gcagcaacac gcccgatctc tgcggttgca ctcatgacgt aatgtggcag tttctctttt
    22321 gccacctcat taatcggtac gcatggcagg cagtgaatct gagccagaaa accatctacc
    22381 agcgttgaat cttcagtcag atcggtaagc agccagattt ctggtgcggt taataaatga
    22441 ggttgagctg ggttcaactt gttccgcaga atctgcacat tcatgccagc acgttctgcc
    22501 agttgcacca ggttgtggcg caatgcgaat gcacgacagg cttcatcaaa atgtggatgt
    22561 ttggaaactt ggtaatcaaa catggtcaat gcctctgatg tatttcagaa tcgaactaat
    22621 taaggtttag attgcattct gaaagcgcat caacggtcat tgctgctatg ttgatcatca
    22681 ctttttcgcg ttttttatct ttgcgcagac gatgacggat aaggcgtcca tcagccaaca
    22741 tgtcattgat ggtatcgatg gatagccctg tcagctcgct atagcgttca atagtcacat
    22801 gaggcgtggt aagagtgatt gaaatgttag gtctcatgat gcaacattcc tcgtttaatg
    22861 atgattaatc aggacgaata cggatcgttt gtattttgtg aacaccataa acatacgatc
    22921 gcacagtgaa atcgtcaaga taaaagttca cttggagtga ccatgaattt ggagaaaggc
    22981 ggacgaggcg ctatagagcg catggtagaa gcttatggat tcaagactcg acaggcgttg
    23041 tgcgatcatt taggaatctc taaaagtaca ctcgccacac gctacatgcg tgactcattc
    23101 ccagcagaat gggtaatcca gtgcgccctt gaaacaggca cctcgcttaa ttggctcaca
    23161 accgggcatg gttcaaagca aacttcaggt aatacaaata ctatggaagt tgctaaatat
    23221 gtattatctg atggggcctt gtgtgaagac ggtttttata ttttcgatag agaatttcta
    23281 ccgtcggcat tcaagaatct ttttgtaatc acagataata attctgaatt tatttgtgat
    23341 aaggaatttg atgatatacg tgatggtaaa tgggtaataa gtattgatgg cgaaataacg
    23401 atccgtgaca ttactcgttt acccggtgga agaatcttcg tcgagggtgg aaacagagcc
    23461 ttcgagtgca agatagaaga tgttgaaata attgggaaaa ttataagttt aacaattaag
    23521 tacgttaggt aataccggga ggaaactatg cttggtaagg tattttttgt ggttttatca
    23581 tgctctttgt tattaaaccc actaactacc tatgctaaaa attatccttg ttctgggaaa
    23641 aagggaggtg tctctcactg tacctccgat ggaaagttcg tttgcaatga tggaactatt
    23701 agtaaatcaa aaaaaatctg tactaaaaac tcgcgataaa ttttgctttt atatctgcgc
    23761 ctaatataac aatgagccgc aggctaaccg caaaagtcac atactcacat agcaaaaaat
    23821 agctaacttc attatggctt cagtgagatg tatggtcgca ggatttcata cattgacact
    23881 ggttatacat acagtaaaaa tgctctctac tggagggcat tttttatggc agtacgaaaa
    23941 ctcaccacag gaaaatggct ttgcgaatgt taccccgccg gacgtagtgg acgtcgtgtg
    24001 cgtaaacaat tcgccaccaa aggcgaagct ctggcttttg agcgtcacac gatggaagaa
    24061 accgaagcaa agccctggct gggcgaatca gtggatcgtc ggacattgaa agacgtgatt
    24121 gagctatggt tcaaactaca tggtaaatct ctgacagctg ggcagcatgt ctatgacaaa
    24181 ctgctgttga tggttgacgc tctgggcaat ccccttgcaa ctgatctaac ctctaaaatg
    24241 tttgcccact atcgagataa acgcctgaca ggtgagatct acttcagcga gaaatggaag
    24301 aaaggagcaa gcccggtcac cattaacctg gagcaaagct atctaagtag tgtttttagc
    24361 gaactatccc gcctgggcga atggtcgtat ccgaacccac tggagaacat gcgaaaattc
    24421 accatcgcag aaaaagagat ggcatggctt acccatgagc agattgttga actgctggct
    24481 gattgcaaac gtcaggaccc aattctggca ctggtagtca agatatgcct aagcacaggc
    24541 gcacgctggc gagaagcaat aaatcttacc cgctcgcaag tgaccaaata ccgaattacc
    24601 tttgtaagaa cgaaggggaa gaaaaacaga agcatcccta tcagtaaaga gctttacgaa
    24661 gagatcatgg cgcttgatgg gttcaatttc tttacagact gctattttca atttttatcc
    24721 gtgatggaaa aaacgtctat cgtgctccct cgcggtcaac tgacacacgt tctgcgccat
    24781 acgtttgcgg cgcacttcat gatgtcgggt ggaaatatcc ttgctttgca aaaaatcctc
    24841 ggacatcacg atataaaaat gactatgcgc tatgcccatt tagcacccga tcatcttgag
    24901 acggctctga gattcaaccc cctcgcaacg ctagaagtgt gatctactgg tgcaaaaata
    24961 acatcatccc ccactagctg agttaattgc gtcagagtaa a
//
LOCUS       DAPHDX010000066        24879 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPHDX010000066 DAPHDX010000000
VERSION     DAPHDX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.066"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(17..166)
                     /locus_tag="RH058_002595"
     CDS             complement(17..166)
                     /locus_tag="RH058_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138169.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            475..1614
                     /gene="wza"
                     /locus_tag="RH058_002596"
     CDS             475..1614
                     /gene="wza"
                     /locus_tag="RH058_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV3138170.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1620..2063
                     /gene="wzb"
                     /locus_tag="RH058_002597"
     CDS             1620..2063
                     /gene="wzb"
                     /locus_tag="RH058_002597"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV3138171.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2066..4228
                     /gene="wzc"
                     /locus_tag="RH058_002598"
     CDS             2066..4228
                     /gene="wzc"
                     /locus_tag="RH058_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV3138172.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAMGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4406..5245
                     /gene="wcaA"
                     /locus_tag="RH058_002599"
     CDS             4406..5245
                     /gene="wcaA"
                     /locus_tag="RH058_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV3138173.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5248..5736
                     /gene="wcaB"
                     /locus_tag="RH058_002600"
     CDS             5248..5736
                     /gene="wcaB"
                     /locus_tag="RH058_002600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV3138174.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5733..6950
                     /gene="wcaC"
                     /locus_tag="RH058_002601"
     CDS             5733..6950
                     /gene="wcaC"
                     /locus_tag="RH058_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV3138175.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDATLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDNIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6925..8142
                     /gene="wcaD"
                     /locus_tag="RH058_002602"
     CDS             6925..8142
                     /gene="wcaD"
                     /locus_tag="RH058_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV3138176.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8153..8899
                     /gene="wcaE"
                     /locus_tag="RH058_002603"
     CDS             8153..8899
                     /gene="wcaE"
                     /locus_tag="RH058_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV3138177.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLALAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8915..9463
                     /gene="wcaF"
                     /locus_tag="RH058_002604"
     CDS             8915..9463
                     /gene="wcaF"
                     /locus_tag="RH058_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV3138178.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            9490..10611
                     /gene="gmd"
                     /locus_tag="RH058_002605"
     CDS             9490..10611
                     /gene="gmd"
                     /locus_tag="RH058_002605"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV3138179.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10614..11579
                     /gene="fcl"
                     /locus_tag="RH058_002606"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10614..11579
                     /gene="fcl"
                     /locus_tag="RH058_002606"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV3138180.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAEGELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGCDYRS
                     VMPTNLYGPHDNFHPNNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11582..12061
                     /gene="gmm"
                     /locus_tag="RH058_002607"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11582..12061
                     /gene="gmm"
                     /locus_tag="RH058_002607"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV3138181.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPMTAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDDVHANSRAYFLAEKRAGVPGL"
     gene            12058..13281
                     /gene="wcaI"
                     /locus_tag="RH058_002608"
     CDS             12058..13281
                     /gene="wcaI"
                     /locus_tag="RH058_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV3138182.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIDKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13284..14720
                     /gene="cpsB"
                     /locus_tag="RH058_002609"
     CDS             13284..14720
                     /gene="cpsB"
                     /locus_tag="RH058_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV3138183.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14913..16283
                     /gene="cpsG"
                     /locus_tag="RH058_002610"
                     /gene_synonym="manB"
     CDS             14913..16283
                     /gene="cpsG"
                     /locus_tag="RH058_002610"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV3138184.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLRDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16338..17732
                     /gene="wcaJ"
                     /locus_tag="RH058_002611"
     CDS             16338..17732
                     /gene="wcaJ"
                     /locus_tag="RH058_002611"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV3138185.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17734..19212
                     /gene="wzxC"
                     /locus_tag="RH058_002612"
     CDS             17734..19212
                     /gene="wzxC"
                     /locus_tag="RH058_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV3138186.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19488..20768
                     /gene="wcaK"
                     /locus_tag="RH058_002613"
     CDS             19488..20768
                     /gene="wcaK"
                     /locus_tag="RH058_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV3138187.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20765..21985
                     /gene="wcaL"
                     /locus_tag="RH058_002614"
     CDS             20765..21985
                     /gene="wcaL"
                     /locus_tag="RH058_002614"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV3138188.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21996..23390
                     /gene="wcaM"
                     /locus_tag="RH058_002615"
     CDS             21996..23390
                     /gene="wcaM"
                     /locus_tag="RH058_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV3138189.1"
                     /translation="MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QATGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGTDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSIGPALSMNFDMRKDIRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23565..24458
                     /gene="galF"
                     /locus_tag="RH058_002616"
     CDS             23565..24458
                     /gene="galF"
                     /locus_tag="RH058_002616"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV3138190.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6107 a         6205 c         6752 g         5815 t
ORIGIN      
        1 gcaatattaa atattgtcaa cctaaagaaa ctcctaaaaa ccatattgaa tgacacttaa
       61 tataattctt aaaaatagcc aattgccgaa ttgttacctt gcctgctatt ccgttagctg
      121 taacacttcc tcctgcatta ttggagagcc aatattcaat taccataatg tcaccttata
      181 ttcggaagtt aatttccgca ataacccttt ctcatcaacg attgtatgca gagagggttt
      241 gaaaaattaa gctaaatcag tgtattggta gctaaaaagc caggggcggt agcgtgtctg
      301 gatgcctgaa agaccagtct gaattattct gtcaatagcc tgcggatgaa atcaattttt
      361 ttaggactga tgccagttaa atttgtattc aattaactgc aaggtaaatg tgcatggcga
      421 cattgctatt aatagtgcac aggataatta ctctgccaaa gtgataaata aacaatgatg
      481 aaatccaaaa tgaaattgat gccattattg gtgtcagtaa ccttgataag cggttgcaca
      541 gtacttccgg gcagcaatat gtcgacgatg ggcaaagacg tcatcaaaca gcaggacgct
      601 gatttcgatc tcgacaaaat ggtgaatgtt tatccgctga ccccgcgcct gattgaccaa
      661 ttacgcccac gcccgaatgt agcgcgcccc aatatgacgc tggaaagtga gatcgcgaat
      721 taccagtatc gcgtcgggcc gggcgacgtt ctgaatgtca ccgtctggga tcacccggaa
      781 ctcaccacgc cagccggtca gtaccgcagc tccagcgaca ccggcaactg ggtacagcct
      841 gacggcacta tgttttaccc gtatatcggc aaggtccacg tagtcgggaa aacgctcgct
      901 gaaatccgca gtgatattac cgggcgctta gcgacgtaca tcgctgaccc gcaggtggat
      961 gttaatatcg ccgccttccg ctcgcaaaag gcctatatct caggtcaggt gaataaatcc
     1021 ggtcaacagg cgatcaccaa cgtaccactg accattctcg acgccatcaa cgccgcaggt
     1081 ggcctgaccg acaccgctga ctggcgcaac gtggtgctaa cacacaatgg tcgtgaagaa
     1141 cgcatttctt tgcaggcgct gatgcaaaat ggcgacctca atcagaaccg actgctttac
     1201 cccggcgata ttctctacgt gccgcgtaat gatgatctga aagtgtttgt gatgggtgaa
     1261 gtgaagaaac agagcaccct gaaaatggac tttagcggca tgaccctgac tgaagccctg
     1321 ggcaatgccg aaggtatcga catgaccacc tccaacgcca gcggcatctt tgtcattcgt
     1381 ccgctgaaag gcgagggcgg gcgtaacggc aagattgcca atatctacca gctggatatg
     1441 tccgatgcga cgtcgctggt gatggcgaca gaattccgcc tgcaacctta tgacgtggtg
     1501 tatgtcacca ccgccccggt ttcccgctgg aaccgtctga tcaatcagtt gctgccaact
     1561 attagcggtg tccgttacat gacggataca gccagcgaca ttcataactg gtaatcgtca
     1621 tgtttaacaa catcttagtt gtctgtgtcg gcaatatttg ccgttccccg acggcggaac
     1681 gcttactgca acgttatcac ccggagctga aagtggagtc cgctggactc ggcgcgctgg
     1741 tcggtaaggg cgctgatcct accgctatca gcgtcgccgc agaacatcaa ctgtctctgg
     1801 aaggtcactg tgcacggcaa atcagccgcc gcctgtgtcg caactatgac ctgattttga
     1861 ccatggaaaa gcgccatatc gaacgcttat gcgagatggc acctgagatg cgcggcaaag
     1921 tgatgctgtt tggtcactgg gataacgaat gtgaaatccc cgatccgtat cgcaaaagcc
     1981 gggaaacgtt tgcagcggtg tacacattac ttgaacggtc tgcccgccag tgggcgcagg
     2041 cattgaacgc agagcaggta taagaatgac agaaaaagta aaacaacatg ccgctccggt
     2101 aacgggcagt gatgaaatcg atattggtcg cctggtcggc accgtcattg aagcgcgctg
     2161 gtgggtgatt ggcatcaccg ctgtatttgc cctctgtgct gtggtttaca ctttcttcgc
     2221 cacgccgatt tatagtgccg acgcactggt acaaatcgaa caaaacagcg gcaattcgtt
     2281 agtgcaggac attggctcgg cattagccaa caaaccgcct gcatcggacg ccgagatcca
     2341 gttgattcgt tcgcgtctgg tgcttggtaa aacggtggac gatctagacc tcgatattgc
     2401 ggtgagcaaa aacacgttcc cgattttcgg tgcgggttgg gatcgcctga tgggacgtca
     2461 gaacgagacg gtgaaagtga ccacctttaa ccgcccgaaa gagatggcgg accaggtgtt
     2521 tacgcttaat gtgctggatg acaaaaacta cactctgagc agtgatggcg gctttagcgc
     2581 ccgtgggcaa gcgggccaga tgctgaaaaa agaaggcgtc acgctgatgg ttgaagccat
     2641 tcacgcccgc ccgggcagtg agtttaccgt caccaaatac tccacgttgg ggatgatcaa
     2701 ccaactgcaa aacagcctga cggtaacgga gaacggcaaa gacgcaggcg tactgagcct
     2761 gacttatacc ggtgaagatc gcgaacagat ccgcgacatt cttaacagca tcgcccgtaa
     2821 ctatcaggaa caaaatattg agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct
     2881 cgcgcaacag ttaccggaag tacgtagccg cctcgatgtt gccgaaaaca aactgaatgc
     2941 cttccgtcag gataaagatt ctgttgatct gccgctggaa gcgaaagcgg tgctcgattc
     3001 gatggtgaac atcgacgcgc agttgaacga actgaccttt aaagaggcgg aaatctccaa
     3061 gctgtacacc aaagttcacc ccgcgtaccg cacgctgctg gagaaacgtc aggcgctgga
     3121 agacgaaaaa gccaaactta atggtcgcgt aacggcgatg ccgaaaaccc agcaggaaat
     3181 tgtccgtctg acccgcgatg tcgagtctgg tcagcaggtc tatatgcaac tgctgaataa
     3241 agagcaggag ctgaaaatca ccgaagccag caccgtcggc gatgtgcgca ttgttgaccc
     3301 ggcaatcacc cagcctggcg tgctgaaacc gaagaaaggg ctgattatcc tcggcgcgat
     3361 tatccttggc ctgatgcttt ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgcgg
     3421 catcgaaagc ccgcaggtgc tggaagaaca cggtatcagc gtctatgcca gcatcccgct
     3481 gtcggaatgg cagaaagcgc gcgatagcgt caaaaccatc aaagggatta aacgctataa
     3541 acagagccag ctactggcga tggggaatcc aaccgatctg gcgattgaag ccatccgcag
     3601 tctgcgtacc agtctgcact tcgcgatgat gcaggcacag aacaatgtgt tgatgatgac
     3661 cggggttagc ccgtcaatcg gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag
     3721 ccagaccaat aaacgggtgt tgttgatcga ctgcgatatg cgcaaaggct acacccacga
     3781 gctgctgggc actaataacg ttaatggcct gtcggaaatt ctgattggtc agggcgatat
     3841 tactaccgct gctaaaccga cctctattgc caaatttgac ctgatcccgc gcggtcaggt
     3901 gccgccaaat ccttctgaac tgttgatgag cgaacgcttt gccgaactgg tgaactgggc
     3961 gagtaaaaac tatgacctgg tgttgattga tacgccgccg attctggcag tgaccgatgc
     4021 ggcaattgtt ggtcgtcatg tcggaaccac gttaatggtg gcgcgttatg cggtcaacac
     4081 cttgaaagaa gtggaaacca gtctgagccg ctttgagcaa aacggtattc cggtgaaagg
     4141 ggtgattctg aactccatct tccgccgcgc cagcgcgtat caggattatg gctattacga
     4201 atacgaatat aagtcggatg cgaaataacg acatccgctg tcggatgcga tgctgacgca
     4261 tcttatccga cctaccttca cgccgcatcc ggcaagcaaa ccagctccgt aggccggata
     4321 aggcgctcac gccgcatccg gcaacagaac ccgtaaacaa gcttataaac cggcagaaca
     4381 tctcgacact ccgggagaac aacctatgaa agacaatccg ctgatttcaa tctatatgcc
     4441 gacctggaac cgccaacaac tggcgattcg ggcgataaaa tcggtcctgc gccaggacta
     4501 cagcaactgg gagatgatca tcgtggatga ttgctccacc tcctgggaac aactgcaaca
     4561 gtacgtcact gccctcaacg atccgcgtat tacctacatt cacaacgaca ttaacagcgg
     4621 ggcgtgcgcg gtacgtaacc aggcgattat gctggcgcaa ggggaataca ttaccgggat
     4681 cgatgacgat gatgaatgga cgcccaaccg cctgagcgtc ttcctcgccc ataaacagca
     4741 actggtcact cacgcctttt tgtacgctaa cgactacgta tgccagggag aagtttattc
     4801 ccaaccagca agcttaccac tgtatcccaa atcgccgtac tcacgccgcc tgttctataa
     4861 acgcaatatc attggcaatc aggtttttac ctgggcgtgg cgtttcaaag agtgcttgtt
     4921 cgataccgaa ctgaaagcag cacaggatta cgacatcttc ctgcggatgg tggtggagta
     4981 tggcgaaccg tggaaagtgg aagaggcgac gcagatcctg catatcaatc acggtgaaat
     5041 gcagatcacc tcgtcaccga aaaaattctc cgggtacttc cacttttacc gcaagcacaa
     5101 agataaattt gaccgtgcaa gcaaaaaata tcagctgttt accctctacc agatccgcaa
     5161 taaacgtatg acctggcgca ctttgctgac gctgctgtcg gtgcgtaacg gcaaacgtct
     5221 ggctgatggt attcgggggc gctaaagatg ctggaagatc tgcgcgccaa cagctggagt
     5281 ttacgcccgt gctgcatggt tcttgcctat cgcgttgctc atttttgttc ggtgtggcgc
     5341 aaaaagaacg tcctcaacaa tctgtgggcg gccccgctgc tggtgctgta tcgcattatc
     5401 accgaatgct ttttcggtta tgaaatccag gccgccgcga ccattggccg ccgctttact
     5461 atccatcacg gttacgccgt agtgatcaat aaaaacgtgg tggcggggga tgatttcacc
     5521 attcgccacg gcgtcactat cggcaatcgt ggtgccgata acatggcatg tccacacatt
     5581 ggcaacggcg tcgaactcgg tgccaacgtc attattcttg gtgatatcac gcttggtaac
     5641 aacgtcaccg tgggtgcggg cagcgtggtg ctcgactctg tcccggacaa cgcgctggtg
     5701 gtgggagaaa aagcgcgagt gaaggtaatt aaatgaatat tttgcaattt aatgtgcgac
     5761 tggcggaagg cggggcagca ggtgtggcgt tagatctcca ccagcgtgcg ctgcaacagg
     5821 ggctggcgtc acattttgtc tacggttacg gcaaaggcgg caaagagagc gtcagccatc
     5881 aaaactatcc gcaggtcatc aaacatacgc cgcggatgac cgcgatggcg aacattgccc
     5941 tgtttcgtct gtttaatcgc gatctgttcg gcaatttcaa tgagttatat cgcaccatta
     6001 ctcgcacacc gggtccggtg gtcctgcatt ttcatgtgct gcacagctac tggttgaatc
     6061 ttaacagcgt ggtgcgcttt tgcgaaaagg tgaaaaacca taaaccggac gccactctgg
     6121 tctggacgct gcacgatcac tggagcgtta ccggacgctg cgcctttacc gacggttgcg
     6181 aaggctggaa aacgggctgc cagaaatgcc cgaccttaaa taactatccg ccggtgaaga
     6241 ttgatcgggc gcaccagctg gtggcgggca aacgccagtt attccgcgag atgctggcgc
     6301 tgggctgtca gtttatttcc cccagccagc atgtggctga cgctttcaat agtctgtacg
     6361 ggccagggcg ttgccggatt atcaataatg gtattgatat ggcaaccgaa gcgattctgg
     6421 cggacttgcc tccggtgcgc gaaacccagg gcaagccgaa aatcgcggtg gtggcgcatg
     6481 acctgcgtta cgacggcaag actaaccagc aactggtgcg tgagatgatg gcgctgggcg
     6541 acaacattga actgcatacc tttggtaagt tctcgccgtt caccgctggc aacgtggtta
     6601 atcacggctt tgaaactgac aagcgcaagt tgatgagcgc gctcaatcaa atggatgcgc
     6661 tggtgttcag ttctcgcgtc gataactacc cgctgatttt gtgtgaggcg ctatcgattg
     6721 gcgtgccggt gatcgccacc catagcgatg cggcgcggga agtgctgcaa aaatccggtg
     6781 gtaaaaccgt cagtgaagaa gaggtgctgc aactggtgca gttaagcaaa ccggaaatcg
     6841 cgcaggcgat atttggtacc acgctggctg agttcagcca acgcagccgc gccgcctaca
     6901 gtggacaaca gatgctggag gagtatgtca acttctatca gaatctgtag ctacctgctg
     6961 ctgccgctga tctacctgct ggttaacgtc aaaatcgccc agcttggtga aagtttcccc
     7021 atcactatcg tcactttttt acctgtcttg ttactgctgt ttttagaacg catcagcgtt
     7081 aaaaaattga tgattgcctt agggattggt gcgggactca cggcgttcaa ctatctgttt
     7141 ggtcagtcgt tggatgccag taaatacgtc acttcaacca tgctgtttgt ctatattgtg
     7201 atcattattg gcatggtgtg gagtattcgc tttaaaacga tttcgccaca caaccatcgc
     7261 aagatattac gtttctttta tctggtggtc gggctggtgg tggcgctggc ggcagtggag
     7321 atggcgcaaa ttatcctcac cggtggcagc agtattatgg agtcgatttc gaaatatctg
     7381 atttacagca acagctatgt gctgaatttc attaaattcg gcggcaagcg cacgacagca
     7441 ctttatttcg aaccggcatt tttcgctctg gcattaatct caatttggct cagcatcaaa
     7501 cagtttggta tcaaaacgcc taaaaccgat gctatgattc tcgcagggat aatattatcc
     7561 ggatcgtttt caggggttat gacctttatc ctgttttatt tgctggagtg ggcatttcaa
     7621 tatctgaata aagaggcgat taagaaaaag ttgccgttag cattgatttc tctggctgta
     7681 ttcctggttg gtgtggtaat cgcgtttcct tatatttcca cccgtctggg cgatttaggt
     7741 acggaaggat cgtcatcgta ttatcgtatt gtcggtccgc tggtaatggt cggttattct
     7801 ttgacccata ttgacggtgt agtcagattt ggctcacttt atgaatatgt cgcatcattc
     7861 ggaatattta acggtgcgga tgtcggaaaa accatagaca atggtttgta tctgctgatt
     7921 atttattttt cctggttcgc agtgttttta tcgctgtggt acatggggaa agtgataaaa
     7981 atgatgatca acgcttttgg tgataaccgc aattttcgcg tgcaattata tctttttact
     8041 ccggtgtcgc tgttttttac cggttcgata tttagcccgg aatatgcatt tttaatcgtc
     8101 tgtccgttta ttttgcgaaa agcgttaaat attacgaggt aagaataaga acatgttgct
     8161 tagcataatc actgtcgcgt ttcgtaacct cgaagggata gtcaaaacac atgcctcgct
     8221 ggcgcatctg gcgctggcgg aagatatcag cttcgaatgg attgttgtcg atggcggttc
     8281 caatgacggc acccgtgagt atctggaaaa tctcaatggt atctataacc tacgctttgt
     8341 cagcgagcca gataacggta tctacgacgc catgaacaaa ggcattacga tggcacaagg
     8401 caagttcgcg ttgtttctca attcgggcga tatttttcat caggatgccg caaattttgt
     8461 ccgtaagtta aaaatgcaaa aagataacat gatgatcacc ggcgatgcgc tgctggattt
     8521 tggcgacggg cataaaatta aacgtagcgc caaaccgggc tggtatattt atcacagcct
     8581 gcccgccagt catcaggcga tatttttccc tgtatccggc ttgaaaaaat ggcgttatga
     8641 cctggagtat aaagtttctt ccgactacgc gctggcagcc aaaatgtata aagccggtta
     8701 tgcatttaaa aaactcaatg gcctggtgtc tgaattttcc atgggtgggg tatctaccac
     8761 caataatatg gaattgtgtg ctgacgcgaa aaaagtccaa cgacaaatat tacatgtacc
     8821 tggcttttgg gctgaattat cctggcattt acgccaacgt actacctcaa agacgaaagc
     8881 cttatataac aaagtctgaa tataaggaga accaatgcaa gatttaagcg gtttctcggt
     8941 gccgaaaggg ttccggggcg gcaacgctat taaagtgcaa ttatggtggg cagtacaggc
     9001 aacaatattt gcctggtcgc cacaagtatt gtatcgctgg cgggcatttt tattacgttt
     9061 attcggcgca aaaataggaa aaaacgtagt tattcgtccg tcagtaaaaa ttacctatcc
     9121 gtggaaatta accttaggtg attacgcgtg ggtcggcgat gacgtcaatt tatataccct
     9181 cggtgaaata accattggcg cacattcggt gatatcgcaa aaaagttatt tatgcaccgg
     9241 tagccacgac catgcaagtc aacatttcac cattaatgct acgcctattg tgattggcga
     9301 gaaatgctgg ctggcaaccg atgtttttgt tgcccctggc gtcacgattg gcgacggcac
     9361 cgtcgtgggt gcacgaagca gtgtttttaa aacgcttccg gcaaatgtgg tttgccgggg
     9421 gaatcccgca gtggtgatac gcgaacgcgt tgaaactgaa taaattcaaa aaatacagag
     9481 gaataagaca tgtcaaaagt cgctctcatc accggtgtaa ccggacaaga cggttcttac
     9541 ctggcagagt ttctgctgga aaaaggttac gaggtgcatg gtattaagcg tcgcgcatcg
     9601 tcattcaaca ccgagcgcgt ggatcatatt tatcaggatc cgcatacctg taacccgaaa
     9661 ttccatctgc attatggcga cctgagtgat acctccaacc tgacgcgtat tttgcgtgaa
     9721 gtgcagccgg atgaagtgta caacctgggc gcaatgagcc acgttgcggt ttcttttgag
     9781 tcaccggaat ataccgctga cgtcgacgcg atgggtacgc tgcgcctgct cgaggcgatc
     9841 cgcttcctcg gtctggaaaa gaaaacccgt ttctatcagg cttccacctc tgaactgtat
     9901 ggtctggtgc aggaaattcc gcagaaagaa accacgccgt tctacccgcg atctccgtat
     9961 gcggtcgcca aactgtacgc ctactggatc accgttaact accgcgaatc ctacggcatg
    10021 tacgcctgca acggtattct cttcaaccat gaatccccgc gccgtggcga aaccttcgtt
    10081 acccgcaaaa taacccgcgc aatcgccaac atcgcccagg ggctggagtc gtgcctgtac
    10141 ctcggcaata tggattccct gcgtgactgg ggccacgcca aagactacgt aaaaatgcag
    10201 tggatgatgc tgcaacagga acagccagaa gatttcgtta ttgctaccgg cgttcagtac
    10261 tccgtgcgtc agttcgtaga aatggcggca gcacagctgg gcatcaaact gcgctttgaa
    10321 ggcacgggcg ttgaagagaa gggcattgtg gtttccgtca ccgggcatga cgcgccgggc
    10381 gttaaaccgg gggatgtgat tatcgccgtt gacccgcgtt acttccgtcc ggctgaagtt
    10441 gaaacgctgc tcggcgaccc gactaaagcg cacgaaaaac tgggctggaa accggaaatc
    10501 accctcagag agatggtgtc tgaaatggtg gctaatgacc tcgaggcggc gaaaaaacac
    10561 tctctgctga aatctcacgg ctacgacgtg gcgatcgcgc tggagtcata agcatgagta
    10621 aacaacgaat ttttatcgct ggtcatcgcg ggatggtcgg ttccgccatc aagcgacagc
    10681 tcgaacagcg cggtgatgtg gaactggtat tacgcacccg cgacgagctg aacctgctgg
    10741 acagccgcgc cgtgcatgat ttctttgcca gcgaacgtat tgaccaggtc tatctggcgg
    10801 cggcgaaagt gggcggcatt gttgccaaca acacctatcc ggcggatttc atctaccaga
    10861 acatgatgat tgagagcaac atcattcacg ctgcgcatca gaacgacgtg aacaaactgc
    10921 tgtttctcgg atcgtcctgc atctacccaa aactggcaaa acagccgatg gcagaaggcg
    10981 agctgttaca gggcacgctg gagccgacca acgagccata cgccattgcc aaaatcgccg
    11041 ggatcaaact gtgcgaatct tacaaccgcc agtatggatg cgattatcgc tctgtcatgc
    11101 cgaccaacct gtacgggccg cacgacaact tccatccgaa taattcgcat gtgatccccg
    11161 cattgctgcg ccgcttccac gaggcgacag cacagaatgc accggacgtg gtggtgtggg
    11221 gcagcggtac gccgatgcgc gaatttctgc atgtcgatga tatggcggcg gcaagcattc
    11281 atgtcatgga gctggcgcac gaagtctggc tggagaacac ccagccgatg ctgtcgcaca
    11341 ttaacgtcgg cacgggtgtt gactgcacta tccgcgagct ggcgcaaacc atcgccaaag
    11401 tggtgggtta caaaggccgg gtggttttcg atgccagtaa accggatggc acaccgcgca
    11461 aactgctgga tgtgacgcgc ctgcatcagc ttggctggta tcacgaaatc tcactggaag
    11521 cggggcttgc cagcacttac cagtggttcc ttgagaatca agaccgcttt cgggggtaat
    11581 gatgttttta cgtcaggaag actttgccac ggtagtgcgc tccactccgc ttgtctctct
    11641 cgactttatt gtcgagaaca gccgcggcga gtttctgctt ggcaaaagaa ccaaccgccc
    11701 ggcacagggt tactggtttg tgccgggagg gcgcgtgcag aaggacgaaa cgctggaagc
    11761 cgcatttgag cggctgacga tggcggaact ggggctgcgt ctgccgatga cagcgggcca
    11821 gttttacggt gtctggcagc acttttatga cgataacttc tccggcacgg atttcaccac
    11881 gcactatgta gtgctcggtt ttcgcttcag agtcgcggaa gaggaactat tactgccgga
    11941 tgagcagcat gacgattacc gctggctgac gccggacgcg ctgctcgcca gtgacgatgt
    12001 tcacgctaac agccgcgcct attttctcgc tgagaagcgt gccggagtac ccggattatg
    12061 aaaatactgg tctacggcat taactactcg ccggagttaa ccggcatcgg caaatacacc
    12121 ggcgagatgg tggaatggct ggcagcacaa ggtcatgagg tgcgggtcat taccgcaccg
    12181 ccgtactacc cgcagtggca ggtgggcgag aactattccg cctggcggta caaacgagaa
    12241 gagggggccg ccacggtgtg gcgctgcccg ctgtacgtgc caaaacagcc gagcaccctg
    12301 aaacgcctgt tacatctcgg cagttttgcc gtcagcagtt tctttcctct gatggcgcaa
    12361 cgtcgctgga agccggatcg cattatcggc gtggtgccaa cgctgttttg cacgccggga
    12421 atgcgcctgc tggcgaaact ctctggtgcg cgtaccgtgc tgcatattca ggattacgaa
    12481 gtagatgcca tgcttgggct gggccttgcc ggaaaaggca aaggcggcaa agtggcacag
    12541 ctggcgacgg cgttcgaacg tagcggactg cataacgtcg ataacgtctc cacgatttcg
    12601 cgttcgatga tgaataaagc catcgataaa ggcgtggcgg cggaaaacgt catcttcttc
    12661 cccaactggt cggaaatcgc ccgttttcag catgttgcag acgccgatgt tgatgcactt
    12721 cgtaaccagc ttggcctgcc ggataacaaa aaaatcattc tttactccgg caatattggt
    12781 gaaaagcagg ggctggaaaa cgttattgaa gctgccgatc gtctgcgcga tgaaccgctg
    12841 atttttgcca ttgtcgggca gggcggcggc aaagcgcggc tggaaaaaat ggcgcagcag
    12901 cgtggactgc gcaacatgca atttttcccg ctgcaatcgt atgacgcttt acccgcactg
    12961 ctgaagatgg gcgattgcca tctggtggtg caaaaacgcg gcgcggcaga tgccgtattg
    13021 ccgtcgaaac tgaccaatat tctggcggta ggcggtaacg cggtgattac cgcagaggcc
    13081 cacacagaac tggggcagct ttgcgaaacc tttccgggca ttgcggtttg cgtagaaccg
    13141 gaatcggtcg aggcgctggt ggcggggatc cgtcaggcgc tcctgctgcc caaacacaac
    13201 acggtggcac gtgaatatgc cgaacgcaca ctcgataaag agaacgtgtt acgtcaattt
    13261 ataaatgata ttcggggata attatggcgc agtcgaaact ctatccagtt gtgatggcag
    13321 gtggctccgg tagccgctta tggccgcttt cccgcgtact ttaccccaag cagtttttat
    13381 gcctgaaagg cgatctcacc atgctgcaaa ccaccatctg ccgcctgaac ggcgtggagt
    13441 gcgaaagccc ggtggtgatt tgcaatgagc agcaccgctt tattgtcgcg gaacagctgc
    13501 gtcaactgaa caaactcacc gagaacatta ttctcgaacc ggcagggcgt aacactgcac
    13561 ctgccattgc actggcggcg ctggcggcaa aacgtcatag cccggagagc gacccgttaa
    13621 tgctggtatt ggcggcggat catgtgattg ccgatgaaga cgcgttccgt gccgccgtgc
    13681 gtaatgccat gccatatgcc gaagcgggca agctggtgac cttcggcatt gtgccggatc
    13741 taccagaaac cggttatggc tatattcgtc gcggtgaagt gtctgcgggt gagcaggata
    13801 cggtggcctt tgaagtggcg cagtttgtcg aaaaaccgaa tctggaaacc gcccaggcct
    13861 atgtggcaag cggcgaatat tactggaaca gcggtatgtt cctgttccgc gccggacgct
    13921 atctcgaaga actgaaaaaa tatcgcccgg atattctcga tgcctgtgaa aaagcgatga
    13981 gcgccgtcga tccggatctt gattttattc gtgtggatga agaagcgttt ctcgcctgcc
    14041 cggaagagtc ggtggattac gcggtcatgg aacgtacggc agatgctgtt gtggtgccga
    14101 tggatgcggg ctggagcgat gttggttcct ggtcttcatt atgggagatc agcgcccaca
    14161 ccgccgaggg caacgtttgc cacggcgatg tgattaatca caaaactgaa aacagctatg
    14221 tgtacgccga atctggcctg gtcaccaccg tcggggtgaa agatctggta gtggtgcaga
    14281 ccaaagatgc ggtgctgatt gccgaccgta acgcggtgca ggatgtgaaa aaagtggtcg
    14341 agcagatcaa agccgatggt cgccatgagc atcgggtgca tcgcgaagtg tatcgtccgt
    14401 ggggcaaata tgactctatc gacgcgggcg accgctacca ggtgaaacgc atcaccgtga
    14461 aaccgggcga gggcttgtcg gtacagatgc accatcaccg cgcggaacac tgggtagtgg
    14521 tcgcgggaac ggcaaaagtc accattgatg gtgatatcaa actgcttggt gaaaacgagt
    14581 ccatttatat tccgctgggg gcgacgcact gcctggaaaa cccggggaaa attccgctcg
    14641 atttaattga agtgcgctcc ggctcttatc tcgaagagga tgatgtggtg cgcttcgcgg
    14701 atcgctacgg acgggtgtaa acgtcgcatc aggcgctgtt tgtcggatgc ggcgtgaacg
    14761 ccttatccaa cctacggttc gggtttgtag tcctgataag acgcggcagc gtcgcatcag
    14821 gcaatgaatg cgaaaccgag atgtaaataa cgacaaaaac aaaattggcc gtttcggtca
    14881 gggccaacta ttgcctgaaa aagggtaacg acatgaaaaa attaacctgc tttaaagcct
    14941 atgatattcg cgggaaatta ggcgaagaac tgaatgaaga tattgcctgg cgcattggtc
    15001 gcgcctatgg cgaatttctc aaaccgaaaa ccattgtgtt aggcggtgat gtccgcctca
    15061 ccagcgaaac cttaaaactg gcgctggcaa aaggtttacg ggatgcgggc gtcgatgtgc
    15121 tggatattgg catgtccggc actgaagaga tctatttcgc cacgttccat ctcggcgtgg
    15181 atggcggcat tgaagttact gccagccata atccgatgga ttacaacggc atgaagctgg
    15241 tgcgcgaagg ggctcgcccg atcagcggcg ataccggact gcgcgatgtc cagcgtctgg
    15301 cagaagccaa cgactttcct cccgttgatg aaaccaaacg cggtcgctat cagcaaatca
    15361 atctgcgtga cgcttacgtt gatcacctgt tcggttatat caacgtcaaa aacctcacgc
    15421 cgctcaagct ggtgatcaac tccgggaacg gcgcagcggg tccggtggtg gacgccatcg
    15481 aagcccgctt taaagccctc ggcgcacctg tggaattgat caaagtgcac aacacgccgg
    15541 acggtaattt ccccaacggt attcctaacc cgctactgcc ggaatgccgc gacgacaccc
    15601 gcaatgcggt catcaaacac ggcgcggata tgggcattgc ctttgatggc gattttgacc
    15661 gctgcttcct gtttgacgaa aaagggcagt ttatcgaagg ctactacatt gtcggcctgc
    15721 tggcagaagc gttcctcgaa aaaaatcccg gcgcgaagat catccacgat ccacgtctct
    15781 cctggaatac cgttgatgtg gtgactgccg ctggcggcac gccggtgatg tcgaaaaccg
    15841 gacacgcctt tattaaagaa cggatgcgca aggaagacgc catctacggt ggcgaaatga
    15901 gcgcccacca ttacttccgt gatttcgctt actgcgacag cggcatgatc ccgtggctgc
    15961 tggtcgccga actggtgtgt ctgaaaggaa aaacgctggg cgaactggta cgcgaccgga
    16021 tggcggcgtt tccggcaagc ggtgagatca acagcaaact ggcgcaaccc gttgaggcga
    16081 ttaaccgcgt cgaacagcat tttagccgtg aggcgctggc ggtggatcgt accgatggca
    16141 tcagtatgac ctttgccgac tggcgtttta acctgcgctc ctccaacacc gaaccggtgg
    16201 tgcggttgaa tgtggaatcg cgcggtgatg tgccgctgat ggaagcgcga acgcgaactc
    16261 tgctgacgtt gctgaacgag taatgtcgga tcttccctta ccccactgcg ggtaaggggc
    16321 taataacagg aacaacgatg acaaatctaa aaaagcgcga gcgagcgaaa accaatgcat
    16381 cgttaatctc tatggtgcaa cgcttttcag atatcaccat catgtttgcc ggactatggc
    16441 tggtttgcga agtgagcgga ctgtcattcc tctacatgca cctgttggtg gcgctgatta
    16501 cgctggtggt gttccagatg ctgggcggca tcaccgattt ttatcgctca tggcgcggtg
    16561 ttcgggcagc gacagaattt gccctattgc tacaaaactg gaccttaagc gtgattttca
    16621 gtgccggact ggtggcgttc aacaatgatt tcgacacgca actgaaaatc tggctggcgt
    16681 ggtatgggct gaccagtatc ggactggtgg tttgccgttc atgtattcgc attggggcgg
    16741 gctggctgcg taatcatggc tataacaagc gcatggtcgc ggtggcgggg gatttagccg
    16801 ccggacaaat gctgatggag agcttccgta atcagccgtg gttagggttt gaagtggtgg
    16861 gcgtttacca cgacccgaaa ccgggcggcg tttctaacga ctgggcgggt aacctgcaac
    16921 agctggtcga ggatgctaaa gcgggtaaga ttcataacgt ctatatcgcg atgcaaatgt
    16981 gcgacggcgc gcgagtgaaa aaactggtcc atcaattggc agacaccacc tgttcggtgc
    17041 tgctgatccc cgatgttttt accttcaaca ttctccattc acgtctcgaa gagatgaacg
    17101 gcgttccggt ggtgccgctg tacgatacgc cgctttccgg ggttaaccgc ctgataaaac
    17161 gtgcggaaga cattgtgctg gcgacgctga tcttgctgct gatctccccg gtgctgtgct
    17221 gtattgcgct ggcggtgaaa ctcagttcac cagggccggt tattttccgc cagactcgct
    17281 atggcatgga tggcaagccg atcaaagtgt ggaagttccg ttcgatgaaa gtgatggaga
    17341 acgacaaagt ggtgacccag gcgacgcaga acgatccgcg cgtcaccaaa gtggggaact
    17401 ttctgcgccg cacctcgctg gatgaactgc cgcagtttat caatgtgctg accggcggga
    17461 tgtcgattgt cggtccacgt ccgcacgcgg tggcgcataa cgaacagtat cgacagctca
    17521 ttgaaggcta catgctgcgc cataaagtga aaccgggcat taccggctgg gcgcagatta
    17581 atggctggcg tggcgaaacc gacacgctgg agaaaatgga aaaacgcgtc gagttcgacc
    17641 ttgagtacat ccgcgaatgg agcgtctggt tcgatattaa aatcgttttc ctgacggtat
    17701 tcaaaggttt cgttaacaaa gcggcatatt gatatgagct tacgtgaaaa aaccatcagc
    17761 ggcgcgaagt ggtcggcgat tgctacggtg atcatcatcg gcctcgggct ggtacagatg
    17821 accgtgctgg cgcggattat tgacaaccac cagttcggtt tgcttaccgt gtcgctggtg
    17881 atcatcgcgc tggcagatac gctttctgac ttcggtatcg ctaactcgat tattcagcga
    17941 aaagaaatca gccaccttga actcaccacg ctgtactggc tgaacgtcgg gctggggctt
    18001 gtggtgtgcg tggcggtgtt tttgttgagt gatgccattg gtgacgtgct gaataacccg
    18061 gacctggcac cgttgattaa aacattgtcg ctggcgtttg tggtgatccc ccacgggcag
    18121 cagttccgcg cgttgatgca aaaggaactg gagttcaaca aaatcggcat gatcgaaacc
    18181 agcgcggtgc tggcgggctt cacttttaca gtggttagcg cccatttctg gccgctggcg
    18241 atgaccgcga tcctcggtta tctggtcaat agtgcggtaa gaacgctgct gtttggctac
    18301 tttggccgca aaatttattg ccccggtctg cacttctcgc tggcgtctgt ggcaccgaac
    18361 ttacgttttg gtgcctggct gacggcggac agcatcatca actatctcaa taccaacctt
    18421 tcaacgctgg tgctggcgcg tattctcggc gcgggcgtgg cagggggata caacctggcg
    18481 tacaacgtgg ccgttgtgcc gccgatgaag ctgaacccga tcatcacccg cgtgttgttt
    18541 ccggcgtttg ccaaaattca ggacgacacc gaaaagctgc gcgtcaactt ctacaagttg
    18601 ctgtcggtgg tggggattat caactttccg gcgctgctcg ggctgatggt ggtgtcgaat
    18661 aattttgtac cgctggtctt tggtgagaag tggaacagca ttattccggt gctgcaattg
    18721 ctgtgtgtgg tgggtctgct gcgctccgta ggtaacccga ttggctcgct gctgatggcg
    18781 aaagcgcggg tcgatatcag ctttaaattc aacgtattca aaacatttct gtttattccg
    18841 gcgattgtta ttggtgggca gatggcgggc gcaatcggcg tcacgcttgg cttcctgctg
    18901 gtgcagatta tcaacaccat tctgagttac ttcgtgatga ttaaaccggt tcttggttcc
    18961 agttatcgcc agtacatcct gagtttgtgg ctgccgtttt atctctcgct gccgacgctg
    19021 gtggtcagtt atgcgctggg cattgtgctg aaagggcaac tggcgctggg gatgctgctg
    19081 gcggtgcaaa tagccgcggg cgtgctggcg tttgtggtga tgattgtgct gtcgcgccat
    19141 ccgctggtgg tggaagtgaa gcgtcagttt tgtcgcagcg aaaaaatgaa aatgctttta
    19201 cgggcggggt gagttgttgt tttcggttcc gacaacggtt gccgggtgca gcgtaaaacg
    19261 ccttatccat cctacggttc tgtgctcggg acctgtaggc ctgataagac gcgtcagcgt
    19321 cgcatcaggc actgaatgcc ggatgcggcg tgaacgcctt atccggccta cggttctgtg
    19381 ctcgggtctt gtaggcctga taagacgcgc cagcgtcgca tcaggcaaaa aacggcaacg
    19441 aacacaacaa acacagcctc acctggcatc aaatttatga ggaaaaaatg aaattactta
    19501 ttctgggcaa ccacacttgc ggcaaccgcg gcgacagcgc catcttgcgc ggcttacttg
    19561 atgccatcaa catcctcaat ccacacgcag aagtggacgt gatgagccgc tatccggtca
    19621 gttcttcctg gctgctcaac cgcccggtaa tgggcgatcc gctgttcctg caaatgaaac
    19681 aacacaacag cgcggcgggc gttgtcgggc gcgttaaaaa agtcctccgt cgccgctatc
    19741 agcatcaggt attgctctca cgcgtcaccg acactggcaa gttgcgtaat atcgccatcg
    19801 cccagggatt cactgacttc gtgcgactgc tgtcaggtta cgacgccatt attcaggtcg
    19861 gcggatcgtt ttttgtcgat ctctacggcg tgccgcagtt tgaacatgcg ctttgcactt
    19921 tcatggcgaa aaagccgctg tttatgattg gtcacagtgt tggcccgttc caggatgagc
    19981 aatttaacca actggcgaac tatgtttttg gtcactgtga cgcgctgatc ctgcgcgaat
    20041 cggtcagcct tgatttgatg aaacgcagca atatcaccac tgcaaaagtg gaacatggcg
    20101 tcgatactgc gtggctggtc gatcaccaca ccgaagattt caccgccagc tatgccgtcc
    20161 aacactggct ggacgttgcc gcacaacaga aaacggtggc aattaccctg cgcgaactgg
    20221 caccgttcga caaacgtctc ggcaccactc aacaagcgta tgaaaaagcc tttgccgggg
    20281 tggtcaatcg cattctcgac gaaggctatc aggttattgc gctctccacc tgtacgggta
    20341 tcgacagcta taacaaagac gaccgcatgg tggcgctcaa cctgcgccag cacatcagtg
    20401 atcctgcccg ttatcacgta gtgatggatg aacttaacga tctggaaatg ggcaaaattc
    20461 tcggtgcctg tgaactcacc gtcggtacgc gcctgcactc tgccattatc tcgatgaatt
    20521 ttgctactcc ggcaattgcc atcaactacg aacataaatc cgccgggatt atgcagcagc
    20581 tgggactacc ggagatggca attgatatcc gtcatttatt agacggcagc ctgcaagcga
    20641 tggttgcgga taccttaggc cagcttccgg cgctgaatgc gcgacttagt gaagccgtca
    20701 gtcgtgagcg tcagacagga atgcagatgg tgcagtccgt actggaacgc atcggggagg
    20761 tgaaatgaag gtcggcttct tcttattgaa atttccgctg tcgtcggaaa ccttcgtcct
    20821 caaccaaatc actgcgttta ttgatatggg ctttgaggtg gagattgtcg cgctgcaaaa
    20881 aggcgacaca caaaacaccc acgcggcatg gacgaaatac aatcttgccg ccagaacccg
    20941 ctggttacag gacgaaccta cgggcaaagt ggcgaaactg cgccaccgag ccagtcagac
    21001 cttgcgcggc attcatcgta aaaatacctg gcaggcgctc aacctcaaac gctatggtgc
    21061 cgagtcgcgg aacctgattt tgtctgccat ttgcggccag gtcgcaacac cattttatgc
    21121 cgatgtcttt atcgctcatt ttggccctgc gggggtaacc gcagcaaaac tacgcgaact
    21181 gggtgtcatt cgcggcaaaa ttgccactat cttccacggt attgatatct ccagtcggga
    21241 agtgctcaac cactacactc ccgaatatca acaactgttt cgccgtggcg acctgatgtt
    21301 accgataagc gatttgtggg ccggaaggct gcaaaaaatg ggctgcccga gggaaaaaat
    21361 cgccgtatcg cgtatgggcg tggacatgac gcgctttagc ccgcgtccgg tgaaagcgcc
    21421 cgcaacgccg ctggaaatca tctccgtcgc acgcttaacc gaaaaaaaag gcctgcatgt
    21481 ggcgatcgaa gcctgccgtc agttgaaaga gcagggcatg gcatttcgct atcgcatcct
    21541 cggcattggc ccgtgggaac gacgcctgcg taccctcatc gaacaatatc aactggaaga
    21601 tgtggtagag atgccgggct ttaaaccgag ccacgaagtg aaagcgatgc tcgacgacgc
    21661 ggatgtcttc ctgttgccat cggtaacggg cgcggatggc gatatggaag gcattccggt
    21721 agcgctgatg gaagcgatgg cggtcggcat tccggtggtt tctactctgc atagcggaat
    21781 accggaactg gtggaggctg acaaatccgg ctggctggtg cctgagaacg atgctcgcgc
    21841 actggcgcaa cgactggcgg cgtttagcca actggacacc gacgaactgg ctccggttgt
    21901 caaacgcgcg cgcgaaaaag tcgaacacga ttttaaccag caggtgatta accgagaact
    21961 cgccagcttg ttgcaggctt tatagaggag aacgtatgcc atttaaaaaa atctcccgac
    22021 gcaccttcct gacggcaagc tccgcgcttg ccttcctcca tacccctttc gcccgcgcac
    22081 ttcccgcccg acaaagcgtt aacattaacg actacaaccc acacgactgg atcgcctcat
    22141 ttaaacaagc cttcggcgaa gggcaaacgg tcgtcgtgcc tgccggattg gtttgcgaca
    22201 atatcaacac cggcatcttt atccctcccg gtaaaacgtt acacatcctg ggaagcctgc
    22261 gcggtaacgg cagagggcgt tttgtcttac aggacggcag ccaggcgaca ggggaggagg
    22321 gcggcagtat gcataacatc accctggatg tgcgtggctc tgactgcacc atcaaagggc
    22381 tggcgatgag cggctttggc ccggtaacgc agatttatat cggcggcaaa aacaaacggg
    22441 tcatgcgcaa cctgaccatc gataacctga ccgttagcca cgctaattac gccatcttac
    22501 ggcagggatt tcataaccag attatcggtg ccaacatcac caattgtaag ttcagtgact
    22561 tacagggcga cgccattgaa tggaacgtgg caattaacga cagtgatatt ttgatctccg
    22621 accatgtcat cgagcgcatc aactgtacta acggcaaaat caactggggc atcggcatag
    22681 gccttgcggg aagtacctac gataacaact acccggaaga ccaggcagtg aaaaactttg
    22741 tcgtggcaaa tatcacgggg acggattgtc ggcagttgat ccatgttgaa aatggtaaac
    22801 attttgttat tcgtaatatc aaagcccgca atatcacgcc ggatttcagt aagaaagcag
    22861 gtattgataa cgcgacggtc gctatttacg gttgtgacaa tttcgtgatt gataatattg
    22921 aaatgattaa tagcgccggg atgttaatcg gctatggtgt aattaaaggc aaatatctct
    22981 cgataccgca aaatttccga gtgaataata ttcaactgga taatacccac cttgcttata
    23041 aattgcgcgg catccaaatc tccgccggga atgctgtctc ctttgtggcg ctgactaaca
    23101 ttgagatgaa gcgtgcatcg ctggagttac acaacaaacc gcaacatctt tttatgcgta
    23161 atatcaaagt gatgcaggaa tcctcaattg gaccagcatt gagcatgaac ttcgacatgc
    23221 gcaaagacat tcgcggcgtc tttatggcga aaaaagaaac actgctgtca cttgcaaatg
    23281 ttcatgcggt gaatgaaaaa gggcaaagct ccgtcgatat cgacaggatt aatcaccata
    23341 ttgttaatgt ggaaaaaatt aactttagat tgccggaacg gagggagtag atttgcgacc
    23401 attcttggaa aaatggagcc atacttagga acaatgctac tgcaatccac aacgaagcgg
    23461 cgtaacatca caagtaattc agtaatcaat tcagggtaat tgatgctggc gaaaaaaatc
    23521 gaacaagcta taattcagca accattttac aggtggatga aataatgacg aatttaaaag
    23581 cagttattcc tgtagcgggt cttgggatgc atatgttgcc tgccaccaag gcgattccca
    23641 aagagatgct accgatcgtc gacaagccaa tgattcagta cattgttgac gagattgtgg
    23701 ctgcagggat caaagaaatc ctcctggtaa ctcacgcgtc caagaacgcg gtcgaaaacc
    23761 acttcgacac ctcttatgaa ttagaatctc tccttgagca gcgcgtgaag cgtcaactgc
    23821 tggcggaagt gcagtccatc tgtccaccgg gcgtgaccat tatgaacgtg cgtcagggcg
    23881 aacctttagg tttgggccac tccattttat gtgcacgacc tgctattggt gacaacccat
    23941 ttgtcgtggt actgccagac gttgttattg atgacgccag cgccgacccg ctgcgttaca
    24001 accttgctgc catgattgcg cgcttcaacg aaacgggccg cagccaggtg ctggcaaaac
    24061 gtatgccggg tgacctctct gaatactctg tcatccagac caaagagccg ctggaccgcg
    24121 aaggtaaagt cagccgcatt gttgaattca tcgaaaaacc ggatcagccg cagacgctgg
    24181 actcagacat catggccgtt ggtcgctatg tgctttctgc cgatatttgg ccggaactag
    24241 aacgcactca accaggtgca tggggacgta ttcagctgac tgatgctatt gctgaactgg
    24301 caaaaaaaca gtctgttgat gcaatgctga tgactggtga cagctacgac tgcggtaaaa
    24361 aaatgggcta tatgcaggcg ttcgtgaagt atggattacg caacctcaaa gaaggggcga
    24421 agttccgtaa aggcattgag aagctgttaa gcgaataatg aaaatctgac cggatgtaac
    24481 ggttgataac aaaattataa cggcagtgaa gattcgtgga gaaagtaatt tgttgcgaat
    24541 attcctgccg ttgttttata taaacaatca gaataacaac gagttagcaa taggatttta
    24601 gtcaaagttt tccaagattt tccttgtttc cagagcggat tggtaagaca attagcgttt
    24661 gaatttttcg ggtttagcgc gagtgggtaa cgctcgtcac atcgtaggca tgcatgcagt
    24721 gctctggtag ctgtaaagcc aggggcggta gcatgggtat tttgaagcat ttatacctcc
    24781 ttattcagtg agaagggaca gattacgtaa cactgctttg ttgtactaaa taattcgcat
    24841 tttatgttca acaatcgaga tattccttat tacctaaaa
//
LOCUS       DAPHDX010000067        23993 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPHDX010000067 DAPHDX010000000
VERSION     DAPHDX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.067"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..3813
                     /gene="yfaL"
                     /locus_tag="RH058_002617"
     CDS             79..3813
                     /gene="yfaL"
                     /locus_tag="RH058_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV3138191.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGMVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFCLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3941..4663)
                     /locus_tag="RH058_002618"
     CDS             complement(3941..4663)
                     /locus_tag="RH058_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV3138192.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4810..7437
                     /gene="gyrA"
                     /locus_tag="RH058_002619"
     CDS             4810..7437
                     /gene="gyrA"
                     /locus_tag="RH058_002619"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV3138193.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7586..9274
                     /locus_tag="RH058_002620"
     CDS             7586..9274
                     /locus_tag="RH058_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV3138194.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDETLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9271..9894
                     /locus_tag="RH058_002621"
     CDS             9271..9894
                     /locus_tag="RH058_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV3138195.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9918..14432
                     /locus_tag="RH058_002622"
     CDS             9918..14432
                     /locus_tag="RH058_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV3138196.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELCLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGAVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14433..16082
                     /locus_tag="RH058_002623"
     CDS             14433..16082
                     /locus_tag="RH058_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV3138197.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLIRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16087..16863
                     /locus_tag="RH058_002624"
     CDS             16087..16863
                     /locus_tag="RH058_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV3138198.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17138..19987
                     /gene="rcsC"
                     /locus_tag="RH058_002625"
     CDS             17138..19987
                     /gene="rcsC"
                     /locus_tag="RH058_002625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV3138199.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20187..20837)
                     /gene="rcsB"
                     /locus_tag="RH058_002626"
     CDS             complement(20187..20837)
                     /gene="rcsB"
                     /locus_tag="RH058_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV3138200.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20854..23526)
                     /gene="rcsD"
                     /locus_tag="RH058_002627"
     CDS             complement(20854..23526)
                     /gene="rcsD"
                     /locus_tag="RH058_002627"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV3138201.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5842 a         5831 c         6554 g         5766 t
ORIGIN      
        1 atttacaaaa gaaagataac ccatattgta taatgcgttt aaatatgccg tcttatataa
       61 aatgatggtc agattaatat gcggattatc tttctacgca aggagtattt atctctactc
      121 ccgtcaatga ttgcatctct tttctctgct aacggtgtcg cggcggccat tgatttatgc
      181 cagggatatg atatcaaagc gagttgtcac gccagcaggc aaagcctttc aggcattacg
      241 caggactgga gtgtcgccga tgggcaatgg ctggtttttt cggatatgac caataatgcc
      301 agcggaggag ccgtattttt gcaacaagga gcggaatttt cactattacc agaaaatgaa
      361 actggaatga ctctgtttgc caataacacc gttacaggag aatataataa cggcggggcc
      421 atatttgcta aagaaaactc aacgctgaat cttactgatg ttattttttc cggtaacgtc
      481 gcaggcggct atggtggcgc aatctattct tctggtacta acgatactgg tgccgtcgat
      541 ttacgtgtca ctaacgccat gtttcgcaat aacatcgcta atgatggcaa aggtggcgca
      601 atttatacca ttaataatga cgtttattta agtgatgtta tttttgataa caaccaggca
      661 tatacatcaa caagttacag tgatggcgat ggcggggcaa tcgatgttac cgataataat
      721 agcgacagca agcatccttc aggttatacg ataataaata acaccgcctt tacaaataac
      781 actgccgaag gttatggcgg ggcgatatat accaatagcg cgacggctcc ctatcttatt
      841 gatatttctg ttgatgacag ctacagccag aacggcggcg tgttagtgga tgagaacaac
      901 agcgcagcag gctatggaga tggtccttcc actgcggcgg gtggctttat gtatctcggc
      961 ttaagtgaag ttacctttga tattgccgac ggaaaaacgc tggttattgg caatacagag
     1021 aatgacggag ctgttgactc tattgctggt accgggttga tcaccaaaac aggttccggc
     1081 gatctggtac ttaatgcaga taacaatgac tttactggtg agatgcagat tgaaaacggt
     1141 gaagttaccc tgggtcgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac
     1201 gatccgcaag actgctacgg tctgacgata gggagtattg ataagtacca gaatcaggca
     1261 gagctaaacg ttggctccac ccaacaaacc tttgtgcact cattgacggg ctttcagaat
     1321 ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggtag ttttgctggc
     1381 accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctacgt gctgtcgggg
     1441 gcgcagtcga tggcgctaac cggcgatata gtcgttgatg atggtgcggt gctttcgctg
     1501 gaaggcgacg cggcagatct cgccgctctc caggacgatc cgcagtcgat cgtgttaaac
     1561 ggcggtgtgc tcgatctctc tgatttctcc acctggcaga gtggcacatc atacaacgat
     1621 ggccttgaag tcagcggcag cagcggaatg gttatcggca gtcaggatgt ggtagatctt
     1681 gcaggtggaa acgatatgca tatcggcggt gacgggaaag atggcgtcta cgtggtggtc
     1741 gatgcgggcg acgggcaggt aagtctggca aacaataata gttatttggg cacaacacaa
     1801 atcgcctccg gtacgctgat ggtgagcgac aactcgcagc ttggagatac ccactataac
     1861 cgccaggtta tctttaccga taagcaacaa gaaagcgtga tggagattac tgccaatgtc
     1921 gatactcgct ctacgacgac tgagcatggg cgtgatattg aaatgcgcgc cgacggtgaa
     1981 gtggcagttg atgcgggggt agacacgcag tggggcgcac tgatggctga cagcagcggg
     2041 cagcatcagg atgagggtag cacatttact aaaacggggg cgggtacgct ggagctgacc
     2101 gccagcggta caacgcagtc ggcggtacgt gtcgaagaag gcaccctgaa aggcgatgtt
     2161 gcggatatct ttccttatgc ttcgtcactg tgggttggtg atggggcgac gttcgttact
     2221 ggcgcggatc aggatattca gtctatcgat gctacttcca gcggcactat cgatatcagc
     2281 gatggtacgg ttttgcgtct gaccgggcag gatacttccg tcgcccttaa tgcctcattg
     2341 tttaacggcg atgggacgct ggtgaatgct accgatggcg tgacgttgac aggtgagctt
     2401 aataccaacc ttgaaactga cagcctgacc tatcttgccg acgtgacggt taatggtgat
     2461 ctgaccaata cgtccggtgc ggttagcctg caaaatggcg tcgctggcga tacgctgacg
     2521 gtaaacggtg attataccgg cggcggtacg ctactgttcg acagcgaatt aaacggcgat
     2581 gactcggtaa gcgatcaatt ggtgatgaac ggtaatactg ctggcaacac aactgtggtg
     2641 gttaactcca ttacagggat tggtgagccg acatcgacag gcattaaagt ggttgatttc
     2701 gcagctgatc ccacgcagtt tcaaaacaat gcacagttct gtctggctgg cagtggctac
     2761 gtcaacatgg gagcgtatga ctacacgcta gtggaagata acaacgactg gtatctgcga
     2821 tcgcaagaag taacgccgcc atcgccacct gatccagacc ctgacccaac acccgatcct
     2881 gacccaacac ccgatccaat ccccgcttac cagccggtgc tgaatgccaa agttggcggt
     2941 tatctcaata atctgcgggc ggcaaatcag gcgtttatga tggagcgacg cgatcatgca
     3001 ggtggcaatg gtcagacgct gaatttacgt gttatcggcg gacgttatca ttacacggcg
     3061 gcagggcaac tggctcagca tgaagacact tctacggtgc agcttagcgg tgacctgttt
     3121 agcgggcgct ggggcgatga tggcgagtgg atgcttgggg cggttggtgg ctacagtgat
     3181 aatcagggcg agagccgctc gaatatgacc ggaactcgcg ccgataacca gaaccacggt
     3241 tatgccgttg ggctgacctc aagctggtat cagcacggta atcagaagca gggggcctgg
     3301 ctggatagct ggctgcaata cgcgtggttt aacaatgatg tttccgaaca agacgatggc
     3361 acagatcatt accactcgtc ggggattatc gcctcgctgg aagccgggta tcagtggtta
     3421 ccggggcgtg gtgtggtgat tgaaccgcag gcgcaggtga tttatcaggg cgtgcagcag
     3481 gatgatttta ccgccgctaa ccatgcgcgc gtgtcacaat cgcaggggga tgatattcag
     3541 acgcggctgg gtttacacag cgaatggcgt accgcggttg gtgtcacacc aacattagat
     3601 ctgaattatt atcacgatcc ccatgcgacg gaaattgaag aagatggcag cactatcagt
     3661 gacgatgcgg tgaagcaacg gggtgaaata aaagtgggag tcacgggcaa tatcagtcag
     3721 cgagtgtccc tgcgcggcag cgtggcgtgg cagaaaggga gtgatgattt tgcccagacg
     3781 gcaggttttt tgtcgatgac ggtgaaatgg taatcgctgt ctgaccatca ctattgccgg
     3841 atgcggcgta aacgccttat ccggcctacg gggagcattt gtaggcctga taagacgcgg
     3901 caagcgtcgc atcaggcatt ggctcggcca aacaacaacc tcacttattc tgcgtgtgca
     3961 gcatatagtt cacatccacg ccggggccga gtttaaaagt attagtgagc gggttgtaat
     4021 gtagcccggt tatatgtcgc tctttaaggc ttgtctgatc tacccagcca agcagttctg
     4081 ccggtttaat aaacttcttc acatcatgcg tgcctttggg caccatgcgc aaaatatatt
     4141 ccgcgccaac caccgccatc agccatgact tgccgttgcg gttaagcgtc gagaaaaaga
     4201 catcgccgcc tggtttcacc agttgcgcac aggctctgac cactgactgc ggatcgggga
     4261 cgtgctccag catctccatg caggtcacca catcatactg cccggcatgt tttgccgcgt
     4321 gctcttccac ggtttcctgc acgtaatcca cctgaatgcc gctttccagt gcgtgcagtt
     4381 ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg ccttcgcgcg
     4441 ccatactctc ggccagaatg ccgccgccac aaccgacatc gagcaccttt ttgccaaata
     4501 aaccgccagc acgctcggca atatagccca gacgcagcgg gttaatgcgg tgcagcggtt
     4561 tgaactcacc ttccagatcc caccagcggg aggcgacggc ttcaaattta gcgatctctt
     4621 cgtggtctac gttatggttt accggcgatt tttcggcatt cattagcact tctactccgt
     4681 aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta taggtttacc
     4741 tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca gtagagggat
     4801 agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga ggaagagctg
     4861 aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct gccagatgtc
     4921 cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt actaggcaat
     4981 gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac
     5041 catccccatg gtgactcggc ggtttatgac acgatcgtcc gtatggcgca gccattctcg
     5101 ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg cgactctgcg
     5161 gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact gatggccgat
     5221 ctcgaaaaag agacggtcga tttcgttgat aactatgacg gcacggaaaa aattcctgac
     5281 gtcatgccga ccaaaattcc taacctgctg gtgaacggtt cttccggtat cgccgtaggt
     5341 atggcaacca acatcccgcc gcacaacctg acggaagtca tcaatggttg tctggcgtat
     5401 atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg tccggacttc
     5461 ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg taccggtcgc
     5521 ggcaaggtat atatccgcgc ccgtgctgaa gtggaagttg acgccaaaac cggacgtgaa
     5581 accattatcg tccacgaaat tccgtatcag gtaaacaaag cgcgcctgat cgagaagatt
     5641 gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac
     5701 aaagacggta tgcgcatcgt gattgaagtg aaacgcgacg cggtcggtga agttgtgctt
     5761 aacaacctct actcccagac ccagttgcag gtttctttcg gtatcaacat ggtggcattg
     5821 caccatggtc agccgaagat catgaacctg aaagacatca tcgcggcgtt tgttcgtcac
     5881 cgccgtgaag tggtgacccg tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct
     5941 catatccttg aagcattagc cgtggcgctg gcgaacatcg acccgatcat cgaactgatc
     6001 cgtcatgcgc cgacgcctgc agaagcgaaa actgcgctgg tggctaatcc gtggcagctg
     6061 ggcaacgttg ccgcgatgct cgaacgtgct ggcgacgatg ctgcgcgtcc ggaatggctg
     6121 gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca agctcaggcg
     6181 attctggatc tgcgtttgca gaaactgacc ggccttgagc acgaaaaact gctcgacgaa
     6241 tacaaagagc tgctggatca gatcgcggaa ctgttgcgta ttcttggtag cgccgatcgt
     6301 ctgatggaag tgatccgtga agagctggag ctggttcgtg aacagttcgg tgacaaacgt
     6361 cgtactgaaa tcaccgccaa cagcgcagac atcaacctgg aagatctgat cactcaggaa
     6421 gatgtggtag tgacgctctc tcaccagggc tacgttaagt atcagccgct ttctgaatac
     6481 gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac gtattaaaga agaagacttt
     6541 atcgaccgac tgctggtggc gaacactcac gaccatattc tgtgcttctc cagccgtggt
     6601 cgcgtctatt cgatgaaagt ttatcagttg ccggaagcca ctcgtggcgc gcgcggtcgt
     6661 ccgatcgtca acctgctgcc gctggagcag gacgaacgta tcactgcgat cctgccagtg
     6721 accgagtttg aagaaggcgt gaaagtcttc atggcgaccg ctaacggtac cgtgaagaaa
     6781 actgtcctca ccgagttcaa ccgtctgcgt accgccggta aagtggcgat caaactggtt
     6841 gacggcgatg agctgatcgg cgttgacctg accagcggcg aagacgaagt aatgctgttc
     6901 tccgctgaag gtaaagtggt gcgctttaaa gagtcttctg tccgtgcgat gggctgcaac
     6961 accaccggtg ttcgcggtat tcgcttaggt gaaggcgata aagtcgtctc tctgatcgtg
     7021 cctcgtggcg atggcgcaat cctcaccgca acgcaaaacg gttacggtaa acgtaccgca
     7081 gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg ttatctccat taaggttact
     7141 gaacgtaatg gtttagtggt tggcgcggta caggtagatg actgcgacca gatcatgatg
     7201 atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg aaatcagcat cgtaggccgt
     7261 aacacccagg gcgtgatcct catccgtact gcggaagatg aaaacgtagt gggtctgcaa
     7321 cgtgttgctg aaccggttga cgaggaagat ctggatacca tcgatggcag tgccgcggaa
     7381 ggggacgatg aaatcgctcc ggaagtggac gttgacgacg agccagaaga agaataattt
     7441 tacttcttca tgccaaaagg gagctatctc ccttgtttga attgaaaagt ccaggctgca
     7501 aagtctgggc ttttgtcgta ttagggcacg gtaaagtttg gctgtgcccg taaaaaatgg
     7561 ctggctatac acaaggaatg tggcaatgag tggtgaaaaa aaggcgaaag gctggcggtt
     7621 ctatggtctt gtaggttttg gcgcaatagc actgctttcc gctggcgtct gggcgttgca
     7681 atatgctggc agtgggccag aaaaaacgtt gtcgccgctg gtggtgcaca acaatctgca
     7741 aatcgatctc aatgagccgg acctctttct cgacagcgac tctctgagcc agcttcccaa
     7801 agatctcctc accattccgt ttctccacga tgttctgagc gaagatttcg ttttctatta
     7861 tcagaatcat gccgatcgtc tgggcattga aggcagcatt cgtcgtattg tctatgaaca
     7921 cgatctcacg ctgaaagata agctcttttc gtcactctta gatcagcccg cgcaggcagc
     7981 gctgtggcac gataaacaag gccatctttc acattatatg gtgctgatcc agcgcagtgg
     8041 tttaagcaaa ctgctggagc cattgttgtt tgccgctacc agcgacagcc agttaagcaa
     8101 aacggaaatc agtagcatca agataaatag tgaaactgtt cctgtttatc agttgcgcta
     8161 taacggcaat aacgctctga tgttcgcgac ttatcaggac aagatgctgg tgttttccag
     8221 cacggatatg ttgtttaaag atgatcagca ggataccgaa gccacggcga tcgcaggtga
     8281 tttgttgagc ggcaaaaaac gctggcaagc aagctttggc ctggaagagc gtgctgctga
     8341 aaaaacgcca gtacgccagc gcatcgtggt cagcgccagg ttgctgggtt ttggctacca
     8401 gcggttaatg ccttcttttg ctggcgtacg cttcgaaatg ggtaacgacg gctggcacag
     8461 ttttgtggcg ttaaatgatg aatccgccag cgtagatacc agtttcgatt ttacgccggt
     8521 ctggaacagt atgcctgccg gggccagctt ctgtgtggtg gtgccgtatt cacacggtat
     8581 tgccgaagag atgctttcgc acatcagtca ggaaaacgac aagttgaatg gggcgttaga
     8641 cggtgccgcg gggctgtgct ggtatgcaga ctcaaaattg caaaccccgc tgtttgtcgg
     8701 tcagtttgat ggcactgccg aacaggcgca attgccaggg aaactgttta cgcaaaatat
     8761 tggtgcgcac gaaagcaaag cgccagaagg tgttttgccg ataagccaga ctcagcaggg
     8821 cgaagcgcaa atctggcgtc gcgaagtgag ttcccgatac ggtcagtatc cgaaagcgca
     8881 ggcggcgcaa cccgatcaat taatgtcgga ttattttttc cgcgtgtcgc tggcgatgca
     8941 aaacaaaacg ctgcttttct ccctcgatga aacgctggtt aataacgccc tgcaaacact
     9001 gaataaaaac cgcccggcaa tggtggatgt aatacccact gatggcatcg ttccgctcta
     9061 tatcaatcca caaggcgtgg cgaaactgct gcgtaacgaa acgttgacca gtctgccgaa
     9121 gaatctcgaa ccggtttttt ataacgccgc acaaacttta ttaatgccga agctggacgc
     9181 tttatctcaa caaccgcgtt atgtcatgaa gctggcccag atggaacccg gtgccgcctg
     9241 gcagtggcta cccataacct ggcagccgct atgaggcacg ggctgctggc gctgatttgc
     9301 tggctgtgtt gtgttgttgc ccatagcgaa atgctgaatg tcgaacaatc cgggctgttt
     9361 cgcgcctggt ttgtacgcat tgcacaagaa cagctccgcc aggggccaag tccacgctgg
     9421 tatcagcagg attgtgcggg cctggtgcga tttgcggcga acgagacgct gaaagttcac
     9481 gatagtaaat ggctaaaaag taacggtttc tctagccagt atttgccgcc agagatgacg
     9541 ctaacacctg gacagcgtca actggcgcaa aactggaatc aggggaacgg gaaaaccggc
     9601 ccctacgtga ccgcgattaa tttgattcag tacaacagcc agtttattgg ccaggacata
     9661 aaccaggcgc tgcctggcga tatgattttt ttcgatcagg gcgatgccca gcacttaatg
     9721 gtctggatgg ggcgttacgt catctaccac accggaagcg ccacgaaaac tgacaacgga
     9781 atgcgcgcag tcagtctgca acaacttatg acatggaagg acacccgatg gatacccaac
     9841 gattccaatc ccaatttcat tggcatttat cgtttaaatt ttctggcgcg atagcagcgt
     9901 gcttgtccct gtctcttgtg ggaacagggc ttgctaatgc tgatgattcg cttccttcca
     9961 gtaactatgc gccgcccgcc gggggaacat tctttttgct tgctgacagc agttttagca
    10021 gcagtgaaga ggcgaaagtg cgactggaag cgccggggcg tgattatcgg cgctatcaga
    10081 tggaagagta cggcggcgtg gacgttcgcc tgtatcgtat tcctgacccg atggcatttt
    10141 tgcgccagca gaaaaacctg catcgcattg tggtgcaacc gcaatatctg ggcgacgggc
    10201 tgaacaatac gctgacctgg ctgtgggata actggtacgg caaatctcgc cgcgtaatgc
    10261 agcgtacttt ctcttctcag tcacggcaga atgtgactca ggcattaccc gaattacatc
    10321 tcggcaatgc cattattaaa ccttcccgtt atgtacagaa caaccagttt tccccgctga
    10381 aaaaatatcc cttggtggaa cagttccgtt atccactatg gcaggctaaa ccggtcgagc
    10441 cgcagcaagg ggtaaaactg gaaggcgcat ccagcaattt catctcgccg cagccgggta
    10501 acatttatat tcctctcggc caacaagagc cgggactgta cctcgtcgag gcgatggttg
    10561 gtgggtatcg ggcgacgacg gtggtgtttg tttccgatac cgtggcgctt agcaaagtgt
    10621 caggcaacga gcttctggtg tggaccgcgg gtaaaaaaca gggtgaagcg aagcccggct
    10681 cagagatctt gtggactgac ggtcttggcg tgatgacccg cggtgtgacc gatgacagcg
    10741 gtaccttgca gttacaacat atatcgccag aacgttcata cattctgggt aaggatgctg
    10801 aaggcggtgt ttttgtctcc gagaacttct tctacgaaag cgaaatctac aacacccgct
    10861 tgtatatttt taccgatcgc ccgctatatc gcgcaggcga tcgtgtcgat gttaaagtga
    10921 tgggccgcga gttccacgat ccgttgcatt catcccccat cgtcagcgcc ccggcgaagc
    10981 tttcggtgct ggacgctaac ggcagtctgt tgcaaaccgt cgatgtcacg ctggatgcgc
    11041 gcaatggcgg gcagggaagt ttccgcctgc cagaaaatgc cgttgccgga ggttatgagt
    11101 tatgtcttgc ttaccgcaat caggtctata gcagcagttt tcgcgtggca aactacatca
    11161 agccacattt cgagattggt ttagctctcg acaaaaaaga gttcaaaact ggcgaagcgg
    11221 tcagcggcaa actgcaactg ctctacccgg atggcgagcc ggtaaaaaat gcccgcgtgc
    11281 agttaagttt gcgcgctcag caattatcaa tggtcggtaa cgatttgcgt tatgccggac
    11341 gtttccccgt gtcgctggaa ggcagcgaaa cggtgtccga cgccagcggt catgtggcgt
    11401 taaatctccc cgccgccgat aaaccgagcc gttatttgtt aaccgtctcc gccagtgacg
    11461 gcgcggcgta tcgtgtcacc accaccaaag agatcctcat tgaacgcggt ctggcgcatt
    11521 actcattaag tactgccgca caatacagta atagcggcga gtcggttgtg ttccgttatg
    11581 ccgcgctgga atcttcaaaa caggttcctg ttacgtatga atggttgcgt ctcgaagacc
    11641 gcacgagcca tagcggagag ctaccgtcag gcggcaaatc ctttaccgtc aatttcgcta
    11701 aacctggcaa ctacaatctg acattacgcg ataaagacgg cttaattctc gctgggttaa
    11761 gtcatgccgt cagcggtaag ggcagcacgg cgcatactgg tacggtagat atcgtggcgg
    11821 ataaaacgct gtaccagcca ggcgaaactg cgaagatgct gattaccttt ccggagccaa
    11881 ttgatgaagc attattgacg ctggaacgcg atcgcgtgga acagcagtcg ctgctttcgc
    11941 atccggcaaa ctggctgacg ttacaacgtt taaacgatac ccagtatgaa gcccgggttc
    12001 cagtgagcaa ttcctttgcg cctaacatca ctttttcggt gctgtatacc cgtaatggtc
    12061 agtacagttt tcagaacgcc gggatcaaag ttgccgttcc tcagcttgat atccgggtga
    12121 aaacggacaa aacccattac cagcctggtg aactggtcaa tgtcgaatta acctcgtcgc
    12181 tgaaaggtaa acctgtttct gcgcagctaa cggtaggcgt ggtcgatgaa atgatctacg
    12241 cgctgcaacc agaaatcgcg ccgaatatcg gcaaattttt ctatccgctg gggcgtaaca
    12301 atgtgcgtac cagctccagt ctgtcgttta tcagctacga ccaggcgctc tccagcgagc
    12361 cggttgcgcc tggcgcaact aaccgcagcg agcggcgagt aaaaatgctt gaacgtccac
    12421 ggcgtgaaga ggtggatacc gcggcatgga tgccgtcact cacaaccgat aaacaaggca
    12481 aagcgtattt cacgttcctg atgcctgatt cgttaacccg ctggcgtatc accgcgcgtg
    12541 ggatgaacgg cgacgggctg gtcgggcagg ggcgtgctta tctgcgttcg gaaaaaaatc
    12601 tctacatgaa gtggagtatg ccaacggtgt atcgcgtggg cgacaaaccg gcggcaggac
    12661 tgtttatctt cagtcagcag gataacgaac cggtggcgct ggtgactaaa tttgcaggcg
    12721 ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg ctggcgcaga
    12781 acattcagca atctggcttg ttaagcgcag aactgcaaca aaatgggcaa gtgcaggaca
    12841 gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag cagaaaaatg
    12901 tcatgctcgg cggtggcgag aacgcgctga tgttgcccga gcaggcgagc aatatccggc
    12961 tacaaagtag tgaaacgccg caggagattt tccgcaacaa tcttgatgcg ttagtcgatg
    13021 aaccgtgggg tggcgtaatc aacaccggta gccgtctgat cccgctcagt ctcgcctggc
    13081 gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg attcaggata
    13141 accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg tggggtgaag
    13201 atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg caggccagcc
    13261 aggcgatcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac agctacgcgg
    13321 agcaggcaga taacatgccg ttattgcatc gggcgctggt gctggcatgg gcgcaggaga
    13381 tgaatttgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc cggcgcggaa
    13441 ctaaaactga agatttctct gaggaagaca cccgcgatat caatgatagc ctgatcctcg
    13501 atacaccgga gtctccactg gcagatgcgg tggcaaacgt cttaaccatg acgttgctga
    13561 aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg tgggataaag
    13621 cggcaaacag caatcagccg ctggcgcaca cggttgtgct gcttaatagc ggtggcgacg
    13681 ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact attgagcgcg
    13741 cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt gttttgcctg
    13801 cgcctgcggg cgcatgggct aaacataagt taactggagg gggcgaagac tggcgttggg
    13861 ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg caaaatgtgc
    13921 aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg
    13981 aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact ctgcagccgg
    14041 tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg cttaccagcg
    14101 agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg ggagccgacg
    14161 ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa cagcaggggc
    14221 aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag
    14281 aactgacggg agcggtcact ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg
    14341 ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt gttgcggcag
    14401 ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc gtctggctat
    14461 tggcgttggt tactctgcca acacttgcgg aggagacgcc gttacaactg gtgctgcgcg
    14521 gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat
    14581 tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt tacgcctggc
    14641 tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc ccggaagagg
    14701 tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta cgttcctgtg
    14761 ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg caatactggc
    14821 aacagcgtca ggcacctgcg tggttagcgt ctctgactac gctgaagcca gaaacctcgg
    14881 taacggttaa atcgctgctc gattcattag cgacgttacc tgcgcaaaac aaagctcagg
    14941 aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggta
    15001 gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa atccgtcagg
    15061 gtggttttgc cggctggtta accgatggta caccgctgtg ggttactggc agtggcacca
    15121 gcaaaacggt actaatccgt tatgcgacgg tgcttaatcg ggttttgcca gtgccgacgc
    15181 aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa
    15241 tcacagcgga aaagagtacg acggcagtaa aacccggcgt acttaatggg cgctatcgtg
    15301 tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg accttactga
    15361 gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac gttgccagag
    15421 ttctggatcg cgaggcgaaa agtacgcccc ctgaggcagc gaaagccatg accgtggcga
    15481 ttcgcacttt tttacagcaa aacgccaatc gggaaggcga ttgcctgacc atccccgaca
    15541 gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga acgatgaccg
    15601 cctggacgca ggatctcatc tatgcaggcg atccggttca ttatcatggc agtcgcgcta
    15661 ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg gagcgttacg
    15721 accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga gctccacgct
    15781 caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc
    15841 ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt
    15901 tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt cgtaacttct
    15961 ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacctggcc tttgatggtt
    16021 atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta ttaatggact
    16081 aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc tgtcgctcac
    16141 agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca tccggcagag
    16201 ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt caacatggca
    16261 gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc tgcggcgggt
    16321 aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag agttgaatcc
    16381 gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt gcaggtcatc
    16441 agcccggatg ggcaaagccg acaaaaaatg cagttttact caacgccggg tacaggaacg
    16501 attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga tctcgatctc
    16561 catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct gaaaaacagt
    16621 ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc catgccagca
    16681 ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag cgaaacggaa
    16741 ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga gaaacaggaa
    16801 acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag ttttgactgg
    16861 tgatccatcc cttcacacaa cctggctctc tcttttttca gataagactg ctgggcgatc
    16921 tgccgacgcc aggatataac cattaacaag cgtcggcaga ttggtcattt cttatttttc
    16981 gtactgagaa ctgtccatct actccggctt tcctggttaa ggcaatggtt tctaagcgat
    17041 acgcattatt gcaatggtga cagaacattg cggtaacacg cttttaccgc taccttaacc
    17101 acactccatc ggtcacctga ggcggagctt cgcccctttg aaataccttg cttcttttcg
    17161 tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc tctggctgtt
    17221 gattgctttt tcatccgttt tctacatcgt taatgcgtta catcagcgag aatcggaaat
    17281 tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac gcacctctga
    17341 tgtgatgaaa gagctgaagt acatcgccga aaatcgctta tcggcagaaa acggtgtgct
    17401 ttccccgcgt ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc tgtttgccga
    17461 ctccgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat tggcgtggtt
    17521 tatgcgctac tggcgcgata atttttctgc ggcttacgat ctcaaccggg tatttttaat
    17581 cggcagtgat aacctctgca tggccaattt cggtctgcgt gatatgccag tggaacgcga
    17641 taccgcgttg aaagctttgc atgaacgcat caataaatat cgaaatgcac cacaagatga
    17701 tagcggcagt aacctctact ggatcagcga aggtccgcgc cctggcgtcg ggtattttta
    17761 cgcgttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg tcgagcagac
    17821 catccggatg gagaactttt tcttaccggg tacgttgccg atgggggtta ccattcttga
    17881 tgaaaatggt cataccctga tttcgcttac cggaccagaa agtaaaatta agggcgatcc
    17941 tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccggg agctggtgct
    18001 gaagaaaaat ctgccaccct catcgctaag catcgtgtat tcggtgccgg ttgataaggt
    18061 gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc ttgccggagc
    18121 tgcattgttt actctcgcgc ggatgtacga gcgacgtatt ttcattccgg cggaaagcga
    18181 cgccctgcga ctggaagaac atgagcagtt caatcgcaag attgtcgcct ccgcgccagt
    18241 gggtatctgt attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg aactggcgca
    18301 tacctatctc aatatgctta cgcatgagga ccgccaacga ctgacgcaaa ttatctgtgg
    18361 gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc aaatcagctt
    18421 cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg atgtttcttc
    18481 gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac aggcgagcca
    18541 gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg cgaacgccgc tgtatggcat
    18601 tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg atcggctggt
    18661 gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata ttctcgattt
    18721 ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt gagttttcac cgcgtgaagt
    18781 gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt taggcttgta
    18841 ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc gtttacagca
    18901 ggtcatctcc aacctgttga gtaacgccat aaaattcacc gataccggct gtatagtttt
    18961 gcatgtccgc gctgatggcg attatctctc tatccgtgtt cgcgataccg gcgtggggat
    19021 tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggtcggaa cgggcgtaca
    19081 gcgaaacttc caggggaccg gtctgggtct ggcgatttgt gaaaaactga tcagcatgat
    19141 ggacggcgat atctcggtag attcagaacc gggaatgggc agccagttta ccgtgcgtat
    19201 tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg gaagggttga gtggtaaacg
    19261 ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca gtttgcagcg
    19321 cagcggcatc gtcgttacaa catacgaagg gcaggaaccg actcccgaag atgtgttaat
    19381 tactgacgag gtagtgagta aaaaatggca gggtagagcg gtagtgacct tctgtcgtcg
    19441 ccatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg tggctgcccc
    19501 gcatgagcta ccggcattgt tggcgcgtat ttatttgatc gagatggaga gcgacgatcc
    19561 tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aatgacgata tgatgattct
    19621 ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg gatcgttggg
    19681 ctatcaatgt aaaaccgcga atgatggtgt cgatgcgctt aatgtactta gcaagaatca
    19741 tattgatatc gtgcttagcg acgtcaacat gccaaatatg gatggttacc gcttgacgca
    19801 acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta atgcgttggc
    19861 tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctgtcga agccggtaac
    19921 gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga aatcgcggga
    19981 atcgtaggac ggataaggtg tttatgccgc atctggcatt cagtgcaaat gccagatgcg
    20041 acgctgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat gggaatcgta
    20101 ggccggataa ggcgtttacg ctgcatccgg caatcggtgc aaatgccaga taagacacta
    20161 acgcgtctta tctggcctac agatgattag tctttatctg ccggacttaa ggtcacagaa
    20221 gagagataat tcagcagggc gatatcgttc tcgacaccca gcttcatcat cgcagatttc
    20281 ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc ggtcaccagg
    20341 aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg cttgtcaccg
    20401 taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt aaatttcttc
    20461 cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg tttcagcacg
    20521 atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt gttcatggtc
    20581 agaacaatga tcgacaggct tgggaaatgg cgcttgatgt acttgattaa ggtaatgcca
    20641 tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgcgc atccagtttc
    20701 ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt cacccactca
    20761 atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc ggcaataatt
    20821 acgttcatat tgttcatgta ataggctacc ttgctacagc aagctcttga cataactgtc
    20881 aatgtcgctg atgtattttt ctattcctgg aacatccttc tcacgaatca gatgttccag
    20941 cgtttcacat aactgcttgc cgggtaccag atttagcatg gcaaatacgc ctttaagacg
    21001 atgagccgtt tgggctaacg cagcaaagtc actggttgct gcttcagtat acagcctctt
    21061 aacatcatcc ggtactgtgt ctacaaagag cgcataatag ccgctggcat ggagttgcgc
    21121 attttcatct ccgcccagag gcgattctgt cacctcttcc tgcgccagtt gcacttcaat
    21181 taattgtaag accgcttcct gcatagcgtt gctcatattg aagttgacgc acaattgacc
    21241 aggcccaatt tcccgtacgc cagactcatc atcgcttaaa agcaagccag aggcagtaag
    21301 attagacgga ttatccgtta aaaagatatc ataatcttga ctaattaatc tttcatcggg
    21361 tgtgatacag gttgcacccc aattttctaa ctggcgagtg acaatattcc gaatttctgc
    21421 cgaagtaaca tccaccatta cgcagacatc atccagtaaa cgctcttctt cctcttcaac
    21481 ttccgggtca gctgcgagca ttttgatatg cacagagtag cgtgtaccaa gcccatcccg
    21541 cgttttgatg tttaaatgac cgcccagttt acgtgccagt tgatcgctca gccagaatgc
    21601 cagcgggtcc gccttgccat agcgatcgtt ttgggtctgg ttgataaacg ggaagtgcaa
    21661 attatccatt tcatgaatac ttacgccttc tcccgtgtcc agaatgcgga acgtcaggcg
    21721 gtcttcggag gactcatcct gatcaacctc aagggtgatt tttcccaatt gcgttgaggt
    21781 cacggcatat tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat cgccgcggcg
    21841 catatcgtgt gctttcagat gattgttaat cagcagttgc agacctttac gcttgatggc
    21901 aggcaacact gaaggcacaa cttcatcaat taaatcctgc acggagaaca gcaccgtctc
    21961 acttttccag ctatcatccg caagcatgtt cgctaactgt atttcatcga ccagccgcac
    22021 cagcacatct gcctgattcg ccagttgttt gctttccggg gcgttgagtt tagccgcgct
    22081 ctccgccagg gactgtgcgg gttctttcag cgcatcgcca atgtttttca taaaggtcat
    22141 ccggccctgc tggtttttct catacagacg ctgcgcctgc ttgagtttct tgtttaccag
    22201 cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcga cctggctgcg
    22261 gaacatgcgg atctcataca gctcgttatt gatcgtcgcc tgaataatcc cctgatgctg
    22321 ttccgccatg gtggtgatgt tttgcagatt caaatgcggc agcaaatgat cggcaatttt
    22381 gttacttatg acagtgcggt tcgattcctg atcgtgaacc agcaggccga gcggcagcag
    22441 tgagactatc tcttcattga ttgcccgtaa aatgcgcaat tcgttattga ccgccgtgtt
    22501 gggtagactt tctgtactgc gactggagaa atggcggaat gtggtatagc caaataacgc
    22561 cagcgccagc aaaccgatgt tcagcagcag tggcagcaga atgttttgca acgtatccag
    22621 caataaggtg ccataaggaa cctgccagac caggcgcata tcggtagagt tgagtgccga
    22681 ggagatttca atcttcgtac tgttaaagtg gatactgacg ctatccgttc cttctttctc
    22741 attatcattg tctcccgttg ccgtcgtgtc tggctcaagg cggaaactgt ccagcggcat
    22801 acccggtggg atcaaatcat taatcggcag atcaaaagcc acgaccgttg ccagatgtcc
    22861 cggctggttg aacgtggtac gcaaggtaaa gtaatgaccg ttctgccagg ccaggcggcg
    22921 catgttagaa aaactttcgc gttcatcgag ggcgttggcc tgttgcaaca tttctgcacg
    22981 acgtgaatca acaatgtcac tgacggtcga ttctttaaat ccggaggtga gatctttgag
    23041 gggcagggtt gagatcagca ccagactgtt atcctgacca ttcaggtaat acatcgacca
    23101 cggtacattt tctgcgcccc acaatgtgtc cagataggtg gacatccgtt gagtcatctc
    23161 aagcgttgag ttgtcgtgag agccaaagat taacgcttcc gttttgcggc gtggtttctc
    23221 cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg aggaggggga
    23281 ggtcgtcgcg gcaatgttgt cgtagatctg ccaggtcacg taacgccagg tatcgacgcg
    23341 cttttggatt gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca gccatgcgtt
    23401 aacggcgctt tgtaccatta cccccatcgt caccagtaac acaatgatca acagtaaaaa
    23461 gaagcgggta atgctccccg gtaggagtga aaagcgggtc gtggccgttg tctctttctg
    23521 acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg atagggcagt ataaagggta
    23581 cagtgtgaaa ttccagactc ttacctgatt gatgattctc tattctcccg tgatatttat
    23641 catcctgtat gggggaaata gatgtacaag cgccattttg ctgtacaaat aacctacaaa
    23701 aagcccaacg aaattaattg caaataaacg aagattgctg gaaattatgc tgatgttaat
    23761 tatttgtgaa atagttaaca agcgttatag tttttctgtg gtagcacaga ataatgaaaa
    23821 gtgtgtaaag aagggtaaaa aaaaccgaat gcgaggcatc cggttgaaat aggggtaaac
    23881 agacattcag aaatgaatga cggtaataaa taaagttaat gatgatagcg ggagttattc
    23941 tagttgcgag tgaaggtttt gttttgacat tcagtgctgt caaatactta aga
//
LOCUS       DAPHDX010000068        23808 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPHDX010000068 DAPHDX010000000
VERSION     DAPHDX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.068"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(106..1554)
                     /gene="cusS"
                     /locus_tag="RH058_002628"
     CDS             complement(106..1554)
                     /gene="cusS"
                     /locus_tag="RH058_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV3138202.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(1544..2227)
                     /gene="cusR"
                     /locus_tag="RH058_002629"
     CDS             complement(1544..2227)
                     /gene="cusR"
                     /locus_tag="RH058_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV3138203.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2384..3763
                     /gene="cusC"
                     /locus_tag="RH058_002630"
     CDS             2384..3763
                     /gene="cusC"
                     /locus_tag="RH058_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV3138204.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDAGRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQ"
     gene            3790..4122
                     /gene="cusF"
                     /locus_tag="RH058_002631"
     CDS             3790..4122
                     /gene="cusF"
                     /locus_tag="RH058_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV3138205.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            4138..5361
                     /gene="cusB"
                     /locus_tag="RH058_002632"
     CDS             4138..5361
                     /gene="cusB"
                     /locus_tag="RH058_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV3138206.1"
                     /translation="MKKNALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            5373..8516
                     /gene="cusA"
                     /locus_tag="RH058_002633"
     CDS             5373..8516
                     /gene="cusA"
                     /locus_tag="RH058_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV3138207.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            8618..9994
                     /gene="pheP"
                     /locus_tag="RH058_002634"
     CDS             8618..9994
                     /gene="pheP"
                     /locus_tag="RH058_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV3138208.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(10062..11309)
                     /gene="ybdG"
                     /locus_tag="RH058_002635"
     CDS             complement(10062..11309)
                     /gene="ybdG"
                     /locus_tag="RH058_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV3138209.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     IDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(11417..12070)
                     /gene="nfsB"
                     /locus_tag="RH058_002636"
     CDS             complement(11417..12070)
                     /gene="nfsB"
                     /locus_tag="RH058_002636"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV3138210.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(12164..12532)
                     /locus_tag="RH058_002637"
     CDS             complement(12164..12532)
                     /locus_tag="RH058_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV3138211.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            complement(12597..12845)
                     /locus_tag="RH058_002638"
     CDS             complement(12597..12845)
                     /locus_tag="RH058_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV3138212.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(12911..14029)
                     /locus_tag="RH058_002639"
     CDS             complement(12911..14029)
                     /locus_tag="RH058_002639"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV3138213.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            14471..14623
                     /gene="hokE"
                     /locus_tag="RH058_002640"
     CDS             14471..14623
                     /gene="hokE"
                     /locus_tag="RH058_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV3138214.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            14974..15126
                     /gene="hokE"
                     /locus_tag="RH058_002641"
     CDS             14974..15126
                     /gene="hokE"
                     /locus_tag="RH058_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV3138215.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(15248..15868)
                     /gene="entD"
                     /locus_tag="RH058_002642"
     CDS             complement(15248..15868)
                     /gene="entD"
                     /locus_tag="RH058_002642"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV3138216.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(16043..18283)
                     /gene="fepA"
                     /locus_tag="RH058_002643"
     CDS             complement(16043..18283)
                     /gene="fepA"
                     /locus_tag="RH058_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV3138217.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFHLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            18526..19728
                     /gene="fes"
                     /locus_tag="RH058_002644"
     CDS             18526..19728
                     /gene="fes"
                     /locus_tag="RH058_002644"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV3138218.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            19731..19949
                     /gene="ybdZ"
                     /locus_tag="RH058_002645"
     CDS             19731..19949
                     /gene="ybdZ"
                     /locus_tag="RH058_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV3138219.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            19946..>23808
                     /gene="entF"
                     /locus_tag="RH058_002646"
     CDS             19946..>23808
                     /gene="entF"
                     /locus_tag="RH058_002646"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV3138220.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIR"
BASE COUNT         5526 a         6064 c         6492 g         5726 t
ORIGIN      
        1 aaataaaagc cttcgatatt tcatatgttt aataagtttt tctctaattt gttgatcaac
       61 aataccagga ggtgatgcac cacgccctga atttgaactt aataatcact ctctctccgg
      121 cagcataatc acaaaccttg tcccccgcgc atctgacgtt accgcaaccg tgcctttatg
      181 cgcgacaaca atcgatttca ctatcgccag cccaataccg ctaccttcac cttttcgctg
      241 gcgggaaggg tcaacgcgat agaaacggtc aaacaatcgc ggtaagtgct cgggcgcaat
      301 gggcgtaccg gggttttcga cggaaacttg cacctgatga ttgaccgtct ggcagcgcac
      361 tacaatagcc tcactgggtg gcgtataacg cagggcatta gaaagcaggt tgcttaacgc
      421 ccgacgcagc atcagcggat cgcccgcaac ctgacatttg tcgccaacaa accgcaactc
      481 cacgccgcga tcttccgcta acgcctcgaa aaaatcgaac actttgccga cttcatccgc
      541 caggttgagc attttctttt cggggattag ctggttgtta tcggcctgag cgagaaacag
      601 catatcgctg accattttcg ccattcgcgt cagctcttcg agattagagt agagcacatc
      661 ttccagttcc ttctggctgc gcgactggct gagggcgatt tccgtttgcg ttatgagatt
      721 agtaattggt gtgcgaattt cgtgagcgat atccgctgag aaattggact ggcgggtaaa
      781 gacatcctca atacgctcga tcatatggtt gaacgacagt accagctgtt ccagctcaat
      841 gggcacggtc tgcgggtcga ggcgaacgtc gagatctttc gaggtaatat tctggatttg
      901 acggctgacg ctgcggatcg gcgcgtgacc tttatgtacc gccaatagca cgataaagac
      961 gatcaggatg ctgatgacag atgcggtcat aataagttta ttcatcaaat catttatgta
     1021 atgaagatga aaatcgatcg aaagcgcgat gtagagcgta taaatcggtt tgccgtccac
     1081 caacgggcca accggcaagt taatcatccg ccagttgctg tgttccatat gcccgtgacc
     1141 gtggcctggc atcatcatcg tcgggccgga aaggagatac acctcaccgc cccgcgcgtc
     1201 tttatcgggt atggcgtcac gcgtaaactc gcggatatcc ggcgcaccgg gggagtgata
     1261 caccgtttta ccatggctat ctgccaggga gatcaacacg ttggaataac cactgacgat
     1321 atcttccagc gtcattaagc gtcgggcttg cgtctcgtca ggatgattaa gtacccgttc
     1381 aagcgtggcg ctaatttctt ttaaatcatt aatatcctgc tcggcaaaat gcacttttac
     1441 tgagtggatc atgatccatg caaaggcgaa aaacgctgcg atggtggcca ggctgataaa
     1501 aaaggtcagg cgggttgcca gcgaaaacgg gcgctgaaat ggcttactga ccatccggca
     1561 cctcaagcat gtaacccacg ccgcgcacgg tctgaattag ctttggctca aagtcgttgt
     1621 cgattttgcc gcgcagccgc ttcaccgcta catcaatggc gttagtgtcg ctgtcgaaat
     1681 tcatgtccca cacctgcgag gcgataagcg agcggggcag cacttcgccc tgatggcgga
     1741 ggaaaaactc cagcagagta aactctttac tggtcagagt gatgcgcgtg ccgctgcggg
     1801 tgactttgcg gctgacgaga tcgaccatca gatcggcaac ctgaaactga ctttcgataa
     1861 tcaccgccgc cccgcgccgc aggagagtgc gcacccgcgc cagcagttca gcaaaagcga
     1921 acggcttcac cagatagtca tccgccccca actccagccc cttgacgcga tgttcaatgg
     1981 tgccaagcgc ggtaagcaac agaatcggca tccctttatt ggcggaacgc aacatgcgca
     2041 cgatatccca gccgttcacg tccggcagca taatatcgag gattatcaga tcataatcac
     2101 cggtcatcgc cagatggtag ccattcaacc cgttgtcggc caaatcgacc acaaaaccgg
     2161 cttcggttaa ccctttggtc aagtattctc cggttttctt ttcatcttcg acaatcaaca
     2221 gtttcatatt tcctccgcat gttgcccggg caattctaga gtagcgggat cagatggcaa
     2281 tcgcttattg gcaaaatgac aattttgtca tttttctgtc accggaaaat cagagcctgg
     2341 cgagtaaagt tggcggcata aaatcaccag aaattatgag cctatgtctc cttgtaaact
     2401 tctgccattt tgtgtggccc ttgcgctaac cggttgttca ctggcaccgg attatcagcg
     2461 tccggcaatg cccgtgccgc agcagttctc actcagccag aacggcctgg ttaacgcagc
     2521 agataactat cagaacgcgg gctggcgcac cttttttgtt gataatcagg tgaagacgct
     2581 gattagcgag gcgctggtga ataaccggga tttgcgcatg gcgacgctga aagtgcagga
     2641 ggcacgggcg caatatcgtc tgaccgatgc cggccgctac ccacagctca atggcgaggg
     2701 cagcggcagc tggagcggta atcttaaagg gaataccgcc acgacacggg agttttcgac
     2761 cggccttaac gccagctttg acctcgattt tttcggtcgc ttaaagaaca tgagcgaagc
     2821 cgagcgacaa aattatttag ccactgagga agctcagcgc gcggtgcata ttctgctggt
     2881 ttctaatgtc gcgcaaagct atttcaatca gcaactggcg tatgcgcaat tgcaaatagc
     2941 cgaagaaacg ctgcgtaatt atcagcagtc atatgcgttt gtcgaaaaac aactgttgac
     3001 cggtagcagc aatgttctgg cgctggaaca ggctcgcggg gtgatagaaa gtacccgcag
     3061 tgacatcgct aaacgtcagg gggaactggc gcaggcgaat aatgcattgc aactgttatt
     3121 gggaagctac ggcaagctgc cgcaagcgca gacagtaaac agcgacagcc tgcaaagcgt
     3181 taaattaccg gcgggcttgt cgtcgcaaat cttattgcag cgcccggata ttatggaagc
     3241 tgaacacgcg ttaatggcgg ctaatgctaa tattggtgct gcgcgtgcgg catttttccc
     3301 ctctatcagc ctgaccagtg gaatatcaac cgccagtagc gatctatcct cattatttaa
     3361 cgccagcagc gggatgtgga attttatacc caaaattgag atccccattt ttaatgccgg
     3421 acgcaaccag gccaatctgg atatcgccga aattcgccag cagcagtcgg tggtgaatta
     3481 tgaacagaaa atccagaacg cctttaaaga agtggcagat gcgctggcat tacgtcaaag
     3541 cctgaacgat caaattagcg cccagcagcg ttatctggcg tcgctgcaaa ttaccctgca
     3601 acgggcacgg gcattatatc agcacggcgc ggtaagttat ctggaagtgc tggatgccga
     3661 gcgctcttta tttgcaaccc gacaaacttt acttgacctg aattatgccc gtcaggttaa
     3721 cgaaatttct ttatataccg cacttggtgg cggttggcag tagtaatttt taactccagg
     3781 agagaataaa tgaaaaaagc actgcaagtc gcaatgttca gtctgtttac cgttattggc
     3841 tttaatgccc aggctaacga acatcatcat gaaaccatga gcgaagtaca accgcaggtt
     3901 attagcgcca ctggcgtggt aaagggtatt gatctggaaa gcaaaaaaat caccatccat
     3961 cacgatccga ttgctgccgt gaactggccg gagatgacca tgcgctttac catcactccg
     4021 cagacgaaaa tgagtgaaat taaaactggc gacaaagtgg cgtttaattt tgtccagcag
     4081 ggcaaccttt ctttattaca ggatattaaa gtcagccagt aacccaggtt taatgagatg
     4141 aaaaaaaacg cgcttattat cggcagcatg atcgcgggcg gtattatttc tgcggcaggt
     4201 tttacctggt ttgcaaaggc tgaaccaccc gcagaaaaaa cgtcgaccgc agaacgtaaa
     4261 atcttattct ggtacgaccc gatgtacccc aatacgcggt tcgataaacc aggtaaatcg
     4321 ccgtttatgg atatggatct ggtgccgaaa tatgccgatg aagagagttc tgcgtctggt
     4381 gtgcgcattg acccgactca gacgcagaat ctgggggtga aaacggctac cgtcacgcgc
     4441 gggccgctga cttttgccca gagtttcccg gcgaatgtca gttacaacga gtatcagtat
     4501 gccattgtgc aggctcgcgc tgccgggttt atcgacaagg tgtatccgct taccgtgggc
     4561 gataaagtac aaaagggcac accgcttctc gacctgacca ttcccgactg ggtggaagcg
     4621 cagagtgagt atttactgct gcgcgaaacg ggcggtacgg cgacccagac tgaaggcatt
     4681 cttgagcggc tgcgactggc gggaatgccg gaggcggata ttcgccgcct gatcgccacg
     4741 caaaaaatcc agactcgctt tacgctcaaa gcacccattg atggtgtgat caccgcgttt
     4801 gatctgcgcg cgggaatgaa tatcgccaaa gataacgtgg tagcgaaaat tcagggtatg
     4861 gacccggtgt gggtcactgc tgcgatcccg gagtctatcg cctggctggt gaaagatgcc
     4921 tcgcagttta cgctcaccgt tccggcacga ccggataaaa cactcaccat ccgcaaatgg
     4981 acgctgctac ctggcgtgga tgccgcgacc cgcacgctgc agctgcgtct ggaagtcgac
     5041 aacgccgacg aggcgctaaa accgggcatg aacgcctggt tgcaactcaa caccgccagc
     5101 gaaccgatgc tgctcattcc gtcacaagcg ctgattgata ccggcagcga acagcgggtg
     5161 attaccgttg atgccgacgg gcgctttgta ccgaaacgcg ttgctgtttt ccaggcatcg
     5221 caaggcgtca ccgcattacg ctctggtctg gcggaaggtg aaaaggtggt ttccagcggc
     5281 ctgttcctga ttgattccga agccaatatt tctggcgcac tggagcggat gcgctctgaa
     5341 agtgctaccc atgcgcattg agggaataac caatgattga atggattatt cgtcgctcgg
     5401 tggcgaaccg ttttttggtg ctgatgggcg cgttgtttct gagcatctgg ggcacctgga
     5461 ccatcattaa cacgccagtg gatgcgctgc cggatctctc cgatgtgcag gtgattatta
     5521 aaaccagcta tcccggtcag gcaccgcaaa tcgttgaaaa tcaggtgact tatccgctaa
     5581 ccaccaccat gttgtcggtg cctggcgcga agactgtgcg cggtttctcg cagtttggag
     5641 actcttatgt gtatgtcatt ttcgaagatg gtaccgatcc gtactgggcg cgttcgcggg
     5701 tgctggagta cctcaaccag gtacagggta agctgcctgc gggagtcagc gccgagctgg
     5761 gaccagatgc cacgggtgtt ggctggatct atgaatatgc actggtggat cgcagcggta
     5821 agcacgatct ggccgattta cgctcattgc aggactggtt tctcaaatat gagctaaaaa
     5881 ccatccctga cgttgcggaa gtggcgtcgg tgggcggcgt ggtgaaagag tatcaggtgg
     5941 ttatcgatcc ccagcgcctg gcgcaatatg gcatcagtct agccgaagta aaaagcgcgc
     6001 tggatgcttc aaaccaggaa gcgggcggtt cgtcgatcga actggcggaa gcggaatata
     6061 tggtgcgcgc cagcggctat ctgcaaacgc tcgacgactt taatcacatc gttttaaaag
     6121 ccagtgaaaa tggcgtgccc gtttatctgc gcgatgttgc gaaggtccag attggcccag
     6181 agatgcgccg gggcattgcc gaactaaacg gcgaaggcga agtggcgggc ggggtggtga
     6241 tcctgcgatc cggcaaaaac gcccgagaag tgatcgccgc cgtgaaggac aaactggaaa
     6301 cgctgaaaag tagtctgccg gaaggcgtgg agatagttac aacatacgat cgcagccagc
     6361 tcattgaccg cgccatcgac aacctcagcg gcaagttgct ggaagagttt attgtggtgg
     6421 cggtagtctg tgcgctgttt ctctggcatg tgcgctcggc gctggtggcg attatttcgt
     6481 tgccgctggg gttgtgtatt gcttttattg tcatgcactt ccagggactg aatgccaata
     6541 ttatgtcgtt gggcggcatt gcgattgccg tcggggcgat ggtcgatgcc gctatcgtca
     6601 tgatcgaaaa tgcacataaa cggctggaag agtggcagca ccagcatcct gacgccacgc
     6661 tggataataa aacgcgctgg caggtgatca ccgatgcatc tgttgaagtg ggtccggcgc
     6721 tatttatcag tctgctgatt atcacgttgt cgtttatccc gatcttcacc ctggaagggc
     6781 aggaagggcg tctgtttggc ccgttggcgt tcactaaaac ctatgcgatg gcgggcgcgg
     6841 cgctgctggc gatcgtagtg atccctatcc tgatggggta ctggatccgt ggcaaaattc
     6901 cgccggaaag cagtaacccg ctcaatcgct ttttgattcg tgtttatcat ccgctgttgc
     6961 tgaaagtact gcactggccg aaaaccacgc tgctggtggc agcgctttcg gtgctgacgg
     7021 ttctctggcc gctcaataaa gttggcgggg aatttttacc gcagatcaat gaaggcgact
     7081 tgttgtatat gccatcgacg ctgccgggga tttccgcagc agaggcggcg agtatgctgc
     7141 aaaaaaccga caagctaatt atgagcgtac ctgaagtggc gcgggtattt ggcaaaaccg
     7201 ggaaagcgga aaccgccacc gattctgctc cgctggagat ggtagaaacg accatccagc
     7261 ttaagccgca ggagcagtgg cggccaggca tgacgatgga caaaatcatt gaggaactgg
     7321 ataacaccgt gcgtctgccg gggctggcga atctgtgggt gccgccaatt cgtaaccgta
     7381 tcgatatgct ctcaaccggc attaaaagcc ccatcggcat taaagtttcc ggcactgtgc
     7441 tggcggatat cgacgcgatg gctgagcaaa ttgaagaagt ggcgcgaacg gtgccaggcg
     7501 tagcttctgc tcttgccgag cggctggaag gtgggcgcta tatcaacgtt gagattaacc
     7561 gtgaaaaagc cgcgcgttac ggtatgacgg tggcggatgt gcagttgttt gtgacttctg
     7621 ctgtgggcgg ggcgatggtt ggcgaaacgg tggaaggaat tgcccgttat ccaattaatc
     7681 tgcgttatcc gcaaagctgg cgcgatagtc cgcaggcact gcgccagctg ccgattctga
     7741 cgccgatgaa gcagcaaatc accctggcag acgtggccga cattaaagtc tctaccggac
     7801 cgtcaatgct gaaaaccgag aatgcgcgcc cgacgagctg gatttatatc gatgcccgcg
     7861 atcgtgacat ggtgtcggtg gttcacgatt tgcaaaaagc gatagctgaa aaagtgcagt
     7921 taaaaccggg caccagcgtg gcattctccg ggcagttcga gctactggag cgcgccaacc
     7981 ataagcttaa actcatggtg ccgatgacgt tgatgattat cttcgtgctg ttgtatctgg
     8041 cgttccgtcg ggtgggcgaa gcgttgctga ttatcagcag cgtaccgttt gcgctggtgg
     8101 gcggcatctg gttgctgtgg tggatgggct ttcatctttc cgtggcgacg ggcactggct
     8161 ttatcgccct cgccggggtc gccgccgaat ttggcgtggt gatgctgatg tatttacgtc
     8221 acgccataga ggccgagccg tcgttgaata atccgcaaac attcagtgag cagaagctgg
     8281 atgaggcgtt atatcacggc gcggtcctgc gcgtgcgccc gaaagcgatg acggtggcgg
     8341 tgattatcgc cggtctgctg ccgattctgt ggggaacggg ggctggttca gaggtgatga
     8401 gccggattgc cgcaccgatg attggcggca tgatcaccgc acctttgctg tcgctgttta
     8461 ttatcccggc ggcgtataag ctgatgtggc tgcaccgaca tcgggtacgg aaataaaagc
     8521 aggatacccc gtttaaccgt gtggattgtg tcttgcgacg atgggcacta aatgttaaaa
     8581 ggtgcccctc aacaaaaaag acacacaggg gaaaggcgtg aaaaacgcgt caaccgtatc
     8641 ggaagatact gcgtcgaatc aagagccgac gcttcatcgc ggattacata accgtcatat
     8701 tcaactgatt gcgctgggtg gcgcaattgg tactggtctg tttcttggca ttggcccggc
     8761 gattcagatg gcgggtccgg ctgtattgct gggctacggc gtcgccggga tcatcgcttt
     8821 cctgattatg cgccagcttg gcgagatggt ggtcgaagag ccggtatccg gttcatttgc
     8881 ccactttgcc tataaatact ggggaccgtt tgcgggcttc ctctctggct ggaactactg
     8941 ggtaatgttc gtgctggtgg gaatggcaga gctgaccgct gcgggcatct atatgcagta
     9001 ctggttcccg gatgttccaa cgtggatttg ggctgccgcc ttctttatta tcatcaacgc
     9061 cgttaacctg gtgaacgtgc gcttatatgg tgaaaccgag ttctggtttg cgctgattaa
     9121 agtgctggcg atcatcggta tgatcggctt tggcctgtgg ctgctgtttt ctggtcacgg
     9181 cggcgagaaa gccagtatcg acaacctctg gcgctacggt ggtttctttg ccaccggctg
     9241 gaatgggctg attttgtcgc tggcggtaat tatgttctcc ttcggcggtc tggagctgat
     9301 tgggattact gccgctgaag cgcgcgatcc ggaaaaaagc attccaaaag cggtaaatca
     9361 ggtggtgtat cgcatcctgc tgttttacat cggttcactg gtggttttac tggcgctcta
     9421 tccgtgggtg gaagtgaaat ccaacagtag cccgtttgtg atgattttcc ataatctcga
     9481 cagcaacgtg gtagcttctg cgctgaactt cgtcattctg gtagcatcgc tgtcagtgta
     9541 taacagcggg gtttactcta acagccgcat gctgtttggc ctttctgtgc agggtaatgc
     9601 gccgaagttt ttgactcgcg tcagccgtcg cggtgtgccg attaactcgc tgatgctttc
     9661 cggagcgatc acttcgctgg tggtattaat caactatctg ctgccgcaaa aagcgtttgg
     9721 tctgctgatg gcgctggtgg tagcaacgct gctgttgaac tggattatga tctgtctggc
     9781 gcatctgcgt tttcgtgcag cgatgcgacg tcaggggcgt gaaacacagt ttaaggcgct
     9841 gctctatccg ttcggcaact atctttgcat tgccttcctc ggcatgattt tgctgctgat
     9901 gtgcacgatg gatgatatgc gcttgtcagc gatcctgctg ccggtgtgga ttgtattcct
     9961 gtttgtggca tttaaaacgc tgcgtcggaa ataaggcatt cacgctacat ccgacaaaac
    10021 gatgtccgct ctcatccatt cgatgagagc ggttttttta attactgctt aaatgcaccc
    10081 gccagagagc gaatatcatt gccggttggc gactgatgaa gtcgcagacc aaactcttcg
    10141 acaatcgcaa atatgtgatc gaatatatca gcctgaatgc tttcatattc cagccacacc
    10201 acggtgttag taaacgcgta gatctcgagc ggtaaaccgt tatcacctgg agccagttgg
    10261 cgtaccatta aggtcatatc tttacgaatc cgcggatggt tacgcagata ttcgttcaga
    10321 taggcacgaa aggttccaat attggtcatt cggcgcaggt ttaatatcga ctccgtagaa
    10381 ccttgctggc gattccactc attaatttcc tgatggcggc tggttaaata aggttttaac
    10441 aagtgcgctt tattcagacg ttgcatttcg tcttcatcaa taaaacgaat actggtgaca
    10501 tcaatactga tactgcgctt aatacgtcgc ccgccagatg ctgacatccc gctccagttt
    10561 ttaaaggagt cagaaaccag agaccaggtg ggaatagtgg taatggtatt gtcccagtta
    10621 cgcactttga cggtggttaa cccaatatcg atcaccgcgc catccgcgcc gtatttcggc
    10681 atctccagcc agtcgcccag tttcagcata tcgttcgcgg aaagctgaat acctgccacc
    10741 agaccaagaa tcggatcttt aaaaaccaac atcagcacgg cagccattgc accaagaccg
    10801 ctgatcagaa tcgctggcga ctgaccaatc agcagcgaga tcatcaaaat gccgaccagg
    10861 attgcgccga tcagtttaat cccctgaaat atccctttca gcggtaactg agatgccgcc
    10921 gggaatttct gcgccagatt caaaataaca tccagcaacg agaagactga aagcagcgca
    10981 tacatcatga tccacaactg cgcgcaggta gtcagaatat ctgccgcttc ggtgcctttt
    11041 tgcagccaga ataccgcctg aatattgacg ataatcccct gcagggtaaa agctaaacgg
    11101 tggaagagtt tattctgggt aatgatttgc aaccaaagcc gtgaactggc gatggcacgt
    11161 ttttcgaagg tccgcagtac cacccaatgc aaaataatat gcaccacgac ggcggtcaga
    11221 aaaataatac cgaaaatcat caccatcgag gtggtgtgat cgatctcaat acccgctaaa
    11281 tcttcaacct gggatattaa atcctgcata acgtctcctt tatacaacag cagcctatga
    11341 tgacggctga aacagggtta tgcaaatcag gagaatctga ggggaaatag ccgggcagat
    11401 gcccggcaag agagaattac acttcggtta aggttatgtt ttgcggcaga cgagatttcg
    11461 gcagcgtagc gttaaaatct tcaacgctgt gatgacctac cggaacaacc accagactgg
    11521 tgtagccttt ctctttcaga ccaaattctg catcgaggat ggcggcgtca aaaccttcga
    11581 tgggtaccgc gtccagaccc agcgccgcca cgccgagcag gaagttaccg acgttgagat
    11641 aaacctgttt tgccatccac tctgcatcat catgcagatc tttacggtgc atatcagcga
    11701 agaacttgcg acctttatcg ttcgcggctt tcgcttccgg cgtggcaaag cggccatcag
    11761 catcttcctg gtcaacaacc agcttcagcc aggcatcgtc catcgcggtt tttgcacaga
    11821 acaccacgac gtgcgaggca tcaagtattt tacgttcgtt gaacacataa ttaccggcag
    11881 cggatttggc aacacgcgct ttaccttctt ccgtgctggc aacaataaaa tgccacggct
    11941 gggagttggt gctggatggg ctgtattgca ggagagtttt gatctgctcg gcctgttccg
    12001 gggtaagttt tttactggca tcaaatgcct tagtggaatg acgctttaag gcgacagaaa
    12061 tgatatccat aaagactcca tgtgaaagta attttgcgtg ccagcagatt acaaggttca
    12121 acgagaaatg gtaagcgaga aaaatgcgct atagatttcc gctttagcct ggacgcactc
    12181 ttttttgatc gcgtttagcc agaccatcaa ccaccagatt ccacgaatcg ttgatcagat
    12241 cgcgaagtaa cgcttcggag atttcctcgc cgggatacac cgaaatccag tgctttttat
    12301 tcatgtgata ccctggctta atgctcgggt atatctgctg atttaacagg gatttttgtg
    12361 gatcggactt cagattgata aaggggacgc cgcgcagctc cgacgacagc ataaaaattt
    12421 tgccgccaat tttaaaaaca tcgaactccg ggccaaaagg ccagcaaagc tcgacaaagg
    12481 gtaactcaag ggccaggcgt ttcgccgttt cgtgcagtga ttgcttatcc ataaacgttc
    12541 ctttaggcga aggagaataa gcaaagtatg ccgcgaagta cggcgataat cgacgtttaa
    12601 tccgccagcg agaaccagcg tcgccagata aagcgcagaa caaaatactc aatagcgccc
    12661 agcactaaaa accacagaca aaacaataaa gtgtaaagct ggctaagatc catcagatgg
    12721 aacatggtca ccagtttttg tgccagcgtc agccccagtg cgggggcggg cagcagcagg
    12781 caagagagga aagccatcag caaaatgcct gctgcggtag tcaaggtttc taaagggtgt
    12841 ttcataacat tgttaaatgt aagttaaaac accattgtca gggatattct tctgtaaggc
    12901 aattccctac ttagtcaccg gcccagatct cacaatgctt tttcaccagc ccaatcagcg
    12961 agccaccatc ggcgacgaaa tcgcgcatca gctgcgcttc attcagaccg ctgacgacct
    13021 ggcgatgcag ggcctcaatc gcgctcgatg cacccatttt atgtgcggaa ggggcgattt
    13081 tttccagcaa tcgcaaggta tcttccgtta gcggtcgacg atcgccagtg tgcggatcgg
    13141 taatgacgcc ttccagccca tagcggcagg cctggaaacg gttgaattta tacagcaggt
    13201 aatctttctc ctgatgttta aacgggcgtt ccgtcagtaa ccagtgggcg gtggcctgaa
    13261 ttaatcccgc catatttacc gcgtggctaa gggttaacgg ggtatccatc acccgaacct
    13321 ccaccgtgcc aaaatgagga ctggggcgaa tatcccagtg cagatcttta atgctgtcga
    13381 tcatcgtggt gtaactcagg cagcgaaaca gggcttcaaa ttgttgccag ttactgaccc
    13441 acggcatcgg gccattatcg ggaaaggcgg aaaaaatatt cggtcgtgag gaggcaaaac
    13501 gcgtatccgt tccctgcata tacggcgacg cggcggaaag ggcgatgaag tgcggcacaa
    13561 accgtgacag gccgtgcagc aaataaatgg cgtcatcgcc actggcgcag ccaacatgga
    13621 catgctgacc aaaaacggtc gcctgctgga tgagatagcc aaagttttcc agcgttcgtt
    13681 gatagcgttc gttttcgcat acctcctgac gctgccattt ctgaaacggg tgcgtgccac
    13741 cgccgcaaat ttccagatga tggtctgcgg ctgcctgcaa tacgactttc tgcatcgctg
    13801 aaaactgccc ggcagcctgg ttgatatcac ggcaaacatc cgtcgccagc tccagcatac
    13861 tttcggtgat atcgtgcttt acctctccgg ccgtgatctc atttttaacc gcgtcaatca
    13921 gcattgaaga gtcctggctt aagtcatagc ccggcggatt aaccacctgc atttccagtt
    13981 caataccgag ggtaaaaggt tcagaaacat gaaaatcggg taatggcata ggtttctctt
    14041 aagttggcgt tttctattca gtatagaagt cggagcggct gggcgagatg cggaagttct
    14101 ggaatgtttc tttttttggt gatggtgact gaagcaattt ggctactttt gcaatgtgac
    14161 aagttatggc acggctggct ggtggcgaag aattttgacg attgaggcat gcagaaaaaa
    14221 acgggttcag cttcagttga tcaaatgggg agtgagaagt ccgaaacagg acttactgta
    14281 taaataaaca gctattttgc taagcaaggg taagataact gcgggtgcct gaagctttcc
    14341 ggtttcaggt ttactctgag gtctggaaag atgaagcccc aggacgatat ttctatcaac
    14401 cctggggcta cctacccaaa cctgaacaat ttggatggta gtcccttctt cgcatggagg
    14461 caatataaac atgctgacga aatatgccct tgcggcagtc atagtgctgt gtttaacggt
    14521 gctgggattt acgcttctgg tcggggattc gctgtgtgag ttcaccgtaa aggaacgtaa
    14581 tattgagttt aaggttgttc tcgcttacga accgaagaag tagccgttgt gcggggagca
    14641 atccccgcat atccggttgt caggtcagga tggtaaggca cctgctttac actttcgccc
    14701 gcggtcagtg atggctgcgg gcgatttcgt tttaagagca atgatgagtt ttgccgacag
    14761 aagtccgaaa caggattcac tgtataaata aacagctatt ttgttgagga agggtaagat
    14821 aacggcgggt gcctgaagct ttccggtttc aggttaactc tgaggtctgg aaagatgaag
    14881 ccccaggaga tatttctatc aatcctgggg ctgccactcc gagcctcaac aactcggatg
    14941 gtagtccctt cttcgcatgg aggcaatata aacatgctga cgaaatatgc ccttgtggca
    15001 gtcatagtgc tgtgtttgac ggtactggga tttacgcttc tggtcggaga ctcgctgtgt
    15061 gagttcaccg taaaggaacg taatattgag tttaaggctg ttctcgctta cgaaccgaag
    15121 aagtagccgt tgtgcgggga gcaatccccg catatccggt tgtcaggtca gagtggtaag
    15181 gcacctgctt tacactttcg cccgcggtca ttgatggctg cgggcgaatc gtaccagatg
    15241 ttgtcaatta atcgtgttgg cacagagtta tgactatttt ttcttttatc tgccagtgca
    15301 cagcaaacat ctcattctca cgatgaatga tgacctgctg tttattccag ctaattatct
    15361 gatagtccag aaaacctgca tcagtttgga tctcacttgc cttaaatgcg ctctctttgg
    15421 cggaaaatgc cagtgtcagc gccagagaaa aggctaaacc gcagtctgcg agtcgctcgt
    15481 gttctgctgg tgtaataatg ttgtctgtca attcggtcgc tgtttgcgca gaaaaaattt
    15541 cttcgatatc aacgccaatc ggttgacggg aaaccacggc caatgccgta gccccgcagt
    15601 ggctaatact gccgtatacc tccgcaggcc acaccggttg gcgtagctcg ccgattgcgg
    15661 gcacacattt atagccatat tcccgcagcg cataaattgc agcgatccgc ccggctaaat
    15721 gttcggcttt gcgcttccgt ccagcgtgtt gcagttgcgc gtaatgcggc agccagagta
    15781 aatcctgctc acaaaaattc gccggatcga actcaacaaa atgcagcgta tgtccggcaa
    15841 aggggaggga ggtatgcgta gttttcatgt cgaccatatt agagactgat gacaaacgct
    15901 aaactgcctg atgcgctacg cttatcaggc ctacatggtc ctgcaatata ttgaattggc
    15961 aagatttttg taggccggat aaggcgttca cgccgcatcc ggcatgaacg acgcgcactt
    16021 tgtcaacaat ctgacgttag catcagaagt gagtgtttac gctcatgtac cacgtacgtc
    16081 ccggctcgtt ataggtatac gccccggcac ccgcgatata gttggcccct gccagatcgc
    16141 ccgtggtctg ggcattaccc gcacgccaca aacgtttgtc gaacaggttg tccacgccgc
    16201 cggtcagact gacattcttc gtcacatccc aggtcgcgct cagacccaca atgctgtaag
    16261 gactgatttc tttggtttcc gggccaaccg ctggctgacc tttgtagttg tattttttcg
    16321 gctgctgctt accgtaccag gtgaaggtcg tttgcatcga caaatcttcc cgcgcctgcc
    16381 agctcagcgt tgagttcaac gtgtactccg ggatgatcga caaacggtcg cccgtggttt
    16441 tgttttcact cttcagcata taagtgatgt tattggtcca catcaccgtt tcgctaactg
    16501 gtacgtttaa cgatccttcc agaccttcaa ccactgcttt cggcacgtta tcccactgat
    16561 agagatcggt gccgactgcg ttttgcccta cagccacata gcccgcttca atcttattgc
    16621 gataatcgtt acggaaccag gtgacgcccg ccagccagcc gtcgcgtttg aactccagac
    16681 caatctcttt gttgatgctg gtttctgctt tcaggtcatc gttaccttgc agatagcagc
    16741 cgcccgcgct ggcatagcag ccctgacctt tactgtagag aatgtagttc gggttagtct
    16801 ggtacaggct cggtgcttta taagcacggg caatgcccat tttgagcgtg aagtcatcgc
    16861 ctaaaccttg cgatatgttc agcgccgggc tccagttatt gccgacaata ctgtgatgat
    16921 cgaaacgcag ccccggcgtt acgatggtgc tgtcagtcag ctccatgttg ttttcggcaa
    16981 acagcgagaa aatttctgct tttgaatacg ggctacggtc ggtggcactc acaccatcaa
    17041 tagcgccgcc ggtgttcgcc ccggtcagcg cctgggtatt ggaactcaag tccttcatcc
    17101 gttgctgatt ccactccgta cccagcgtca gcgtctggtt aacgaggaaa tcaatcggca
    17161 ggttaacttc gctgtgcagc atcacgtcat cgagatcgat atcgacgaaa tcctgtgtcg
    17221 ctttttcgtt aaatttccct tcggtaccgc ccgccagacc ttccggaata cgcgagttac
    17281 gggtgtgttc atactgcacc cagttgctgg tggtcacgcc gttatcccag ccaccgttcc
    17341 aggtcagcgc gtagttctgg cgatacaggc ggttggtttc atcgccatat ttcgagcgag
    17401 tgtaagcgtc agagttggtg ttctgggtat cgcccgcata caggttaccc tggcggctgt
    17461 aacccgcttc cagttccagc gattgcaatg gtgcgaagtc ccagcgcacc acgccgttaa
    17521 tatctttatt gatcacccct tcgcgcccgg ctggtaacgt cgtggcatac gttccggcac
    17581 gcgcggactg atggccctgg ttgatatccc acgcgtcagc ctgggttttg tcgaggttgc
    17641 catacaagtg gaagctgaat tcgtcgccca gcggaccggt caggctgaag ttggtgcgtt
    17701 tagtggcacc ttcctcttta tgttctggtg cattgaaata agcgtcccag gagccgtgcc
    17761 actcaccgct gccttttttg gtaatgatgt taaccacgcc gcccgccgcg ccgttgccat
    17821 aacgcgcagc tgccggacca cgcagaactt caatacgttc aatcatttca ggtggcaccc
    17881 aggaggtatc accacgggta tcgcgctcgc cacgccagcc ctgacgcacc gagttacggc
    17941 tgcttaccgg cttgccgtca atcaaaatca gcgtgttttc cggacccata ccgcgaatat
    18001 caatctgtcg gttattccca cgctgaccac tggtggagtt accggtcagg ttaacgcctg
    18061 gcatggtacg gatgatctcc gacacatcgc gggcaacggg gtttttgcgg atttcatctg
    18121 cggtgatggt cgaaacgcca ggcgcctgta agttctgctc ggcggcggta acgacaatag
    18181 tatcgtcatg tgaaacagga gtatcggtcg gctcttgtgc ctgtgctacc ccataaatcc
    18241 ccagattgac caacaaggcc agggaatgaa tcttcttgtt cattgtttta ttcctgcatt
    18301 tttgccacga attgcaactg tcgggcatgg tcgtcctcaa cacgacgcat cccgctaccg
    18361 cgaaaacctt tgatcctgaa agacacgcag tgcagttggt taataaatgt ccgcgcttcc
    18421 cacggcgcgc cattacgcta ttgcaaatgc aaatagttat caataatatt atcaatatat
    18481 ttctgcaatc aatgaaaaat tgcacagtaa acatggggtt atggtgtgac ggcgttaaaa
    18541 gtaggaagtg agagctggtg gcagtcgaaa catggcccgg aatggcagcg tctgaatgac
    18601 gaaatgtttg aggtcacttt ctggtggcgt gatccccaag gttctgaaga atactcgacg
    18661 ataaagcgcg tatggatcta catcactggt gtgaccgatc atcatcagaa cagccagccc
    18721 cagtcgatgc agcgaattgc aggtactgat gtctggcagt ggacgacaca actcaatgcc
    18781 aactggcgcg gcagctactg ctttattccc accgaacgcg atgacatttt ttctgcacca
    18841 tcccccgatc gcctcgaatt acgtgaaggc tggcgaaaac tattacccca ggcaatagcc
    18901 gatccgctga atccacaaag ctggaaaggc gggcgagggc acgctgtttc tgcactcgaa
    18961 atgccgcaag cgcctctgca accgggatgg gattgtccgc aagcgccaga aatacctgcc
    19021 aaagaaatta tctggaaaag tgaacggttg aaaaattcac ggcgtgtatg gatttttacc
    19081 accggcgatg caacagcaga agaacgcccg ctggcagttt tgctcgatgg cgaattttgg
    19141 gcgcaaagta tgcccgtctg gccagtgctg acttcgctga cccatcgtca gcaacttcct
    19201 cccgccgtgt atgtgttgat cgacgctatc gacaccacgc accgcgccca cgaactgccg
    19261 tgtaatgcgg atttctggct ggcagtacag caagagttat tacccctggt gaaagctatt
    19321 gcccctttta gcgatcgcgc cgatcgcacc gtggtcgccg ggcagagttt tggtgggctg
    19381 tccgcgctgt atgccggact gcactggcct gaacgctttg gctgtgtatt aagccagtca
    19441 ggatcgtact ggtggccgca tcggggcggg cagcaagagg gcgtgttact tgaaaagctt
    19501 aaagctggtg aagttagcgc cgaaggtctg cgcattgtgc tggaagcggg tattcgcgag
    19561 ccgatgatta tgcgggccaa tcaggcgctg tatgcgcaat tacaccccat aaaagaatcc
    19621 attttctggc gtcaggttga cggcggacat gatgcgcttt gttggcgcgg tggcttgatg
    19681 caggggctaa tcgacctctg gcaaccactt ttccatgaca ggagttgaat atggcattca
    19741 gtaatccctt cgatgatcca cagggagcgt tttacatttt gcgcaatgcg caggggcaat
    19801 tcagtctgtg gccgcaacaa tgcgccttac cggcaggctg ggacattgtg tgtcagccgc
    19861 agtcacaggc gtcctgccag cagtggctgg aagtccactg gcgtactctg acaccggcca
    19921 atttcaccca gttgcaggag gcacaatgag ccagcattta cctttggtcg ccgcacagcc
    19981 cggcatctgg atggcagaaa aactgtcgga tttaccctcc gcctggagcg tggcgcatta
    20041 cgttgagtta actggagatg ttgatgcgcc attactggcc cgcgcggtgg ttgtcggact
    20101 agcgcaagca gatacgttgc ggatgcgttt tacggaagat aacggcgaag tctggcagtg
    20161 ggtcgatgat gcgctgatat tcgaactgcc agaaattatc gacctacgaa ccaatattga
    20221 tccgcacggt actgcgcagg cattaatgct ggcggatttg caacaagatt tgcgcgtcga
    20281 tagcggtaaa ccactggtct ttcatcagct gatacatgtt gcggataacc gctggtactg
    20341 gtatcagcgt tatcaccatt tgctggtcga tggcttcagt ttcccggcca ttactcgcca
    20401 gatcgccaat atttactgca catggctgcg tggcgaacca acgcctgctt cgccgtttac
    20461 gcctttcgct gatgtagtgg aagagtacca gcaataccgc gaaagcgaag cctggcagcg
    20521 tgatgcggca ttctgggcag aacagcgtcg tcaactgccg ccgcccgcgt cactttctcc
    20581 ggcaccttta gcggggcgca gcgcttcggc agatattctg cgcctgaaac tggaatttac
    20641 cgacggggaa ttccgccagc tggctacgca actttcaggt gtgcagcgta ccgatttagc
    20701 ccttgcgctg gcagccttgt ggctggggcg attgtgcaat cgcatggact atgccgccgg
    20761 atttatcttt atgcgtcgac tgggctcggc ggcgctgacg gctagcggac ccgtgctcaa
    20821 cgttttaccg ttgggtattc acattgcggc gcaagaaacg ctgccggaac tggcaacccg
    20881 actggcagca caactgaaaa aaatgcgtcg tcatcaacgt tacgatgccg aacaaattgt
    20941 ccgtgacagc gggcgagcgg caggagagga accgctgttc ggtccggtac tcaatatcaa
    21001 ggtatttgat taccaactgg atattcctgg tgttcagacg caaacccata ccctggcaac
    21061 cggtccggtt aatgaccttg aactggccct gttcccggat gaacacggtg atttgagtat
    21121 tgagatcctc gccaataaac agcgttacga tgagccaacg ttaatccagc atgctgaacg
    21181 cctgaaaatg ctgattgccc agttcgccgc ggatccggcg ctgttgtgcg gcgatgtcga
    21241 tattatgctg ccaggtgagt atgcgcagct ggcgcagatc aacgccactc aggttgagat
    21301 tccagaaacc acgctgagcg cgctggtggc agaacaagcg gcaaaaacac cggatgctcc
    21361 ggcgctggca gatgcgcgtt acctgttcag ctatcgggaa atgcgcgagc aggtggtggc
    21421 gctggcgaat ctgctgcgtg agcgcggcgt taaaccaggg gacagcgtgg cggtggcact
    21481 accgcgctcg gtctttttga ccctggcact ccatgcaata gttgaagctg gcgcggcctg
    21541 gctaccgctg gataccggct atccggacga tcgcctgaaa atgatgctgg aagatgcgcg
    21601 tccgtcgctg ttaattacca ccgacgatca actgccgcgc tttagcgatg ttcccaattt
    21661 aacaagcctt tgctataacg ccccgcttac cccccagggc agtgcgccgc tgcaactttc
    21721 acaaccgcac cacacggctt atatcatctt tacctctggc tccaccggca ggccgaaagg
    21781 ggtaatggtc gggcagacgg ctatcgttaa ccgcctgtta tggatgcaaa atcactaccc
    21841 gcttacaggc gaagatgtcg ttgcccaaaa aacgccgtgc agttttgatg tctcggtgtg
    21901 ggagtttttc tggccgttta tcgcaggggc aaaactggtg atggctgaac cggaagcgca
    21961 ccgtgacccg ctcgctatgc agcaattctt tgccgaatat ggcgtaacga ccacgcactt
    22021 tgtgccgtcg atgctggcgg catttgttgc atcgctgacg ccgcaaaccg ctcgccagaa
    22081 ttgcgcgacg ttgaaacagg ttttctgtag tggtgaggcc ttaccggctg atttatgccg
    22141 cgaatggcaa cagttaaccg gcgcgccgtt gcataacctg tatggcccga cggaagcggc
    22201 ggtagatgtc agctggtatc cggcttttgg cgaggaactg gcacaggtgc gcggcagcag
    22261 tgtgccgatt ggttatccgg tatggaatac gggtctgcgt attcttgatg cgatgatgca
    22321 tccggtgccg ccgggtgtgg cgggtgatct ctatctcacc ggcatccaac tggcgcaggg
    22381 gtatcttgga cgacccgatc tgaccgccag ccgttttatt gccgatcctt ttgccccagg
    22441 tgaacggatg tatcgtaccg gagacgtcgc ccgctggctg gataacggcg cggtggagta
    22501 cctcgggcgc agtgacgatc agctaaaaat tcgcgggcag cgtatcgaac tgggcgaaat
    22561 cgatcgcgtg atgcaggcgc tgccggatgt caaacaagcc gttacccacg cctgtgtgat
    22621 taacctggcg gcagccaccg gtggtgatgc gcgtcagttg gtgggctatc tggtgtcgca
    22681 atcaggtctg ccgttggata ccagcgcatt acaggcacag cttcgcgaaa cattgccgcc
    22741 gcatatggtg ccggtggttc tgctgcaact tccacagtta cctcttagcg ccaacggcaa
    22801 gctggatcgc aaagccttac cgttgcctga actgaagaca caagcgtcgg gacgtgcacc
    22861 gaaagcgggc agtgaaaaga ttatcgccgc ggcattcgcg tcgttgctgg gttgtgacgt
    22921 gcaggatgcc gatgctgatt tctttgcgct gggcggtcat tcgctactgg caatgaaact
    22981 ggcagcgcag ttaagtcgac agtttgctcg tcaggtgacg ccggggcagg tgatggtcgc
    23041 gtcaaccgtc gccaaactgg caacgattat tgatggtgaa gaggacagct cccggcgcat
    23101 gggattcgaa accattctgc cgttgcgtga aggtaatggc ccgacgctgt tttgtttcca
    23161 tccggcgtcc ggttttgcct ggcagtttag cgtgctctcg cgttatctcg atccacaatg
    23221 gtcgattatt ggcattcagt cgccgcgccc tcatggaccc atgcagacag cgacaaacct
    23281 ggatgaagtc tgcgaagcgc atctggcaac gttacttgag caacaaccgc acggccctta
    23341 ttacctgctg gggtattccc ttggcggtac gctggcgcag ggtattgcgg cgcgactgcg
    23401 tgcccgtggc gaacaggtgg catttcttgg tttgctggat acctggccgc cagagacgca
    23461 aaactggcag gaaaaagagg ctaatggtct ggacccggaa gtgctggcgg agattaaccg
    23521 cgaacgcgag gccttcctgg cggcacagca gggaagtact tcaacggagt tgtttaccac
    23581 cattgaaggc aactacgctg atgctgtgcg cttgttaacc accgcccata gcgttccgtt
    23641 tgatggaaaa gcaacgttat ttgttgctga gcgcacactt caggaaggta tgagtcccga
    23701 acgtgcctgg tcgccgtgga ttgcggagct ggatatctat cgtcaggatt gtgcgcatgt
    23761 ggatattatc tctccagggg cgtttgtaaa aattgggccg attattcg
//
LOCUS       DAPHDX010000069        23803 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPHDX010000069 DAPHDX010000000
VERSION     DAPHDX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.069"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..684
                     /gene="napF"
                     /locus_tag="RH058_002647"
     CDS             190..684
                     /gene="napF"
                     /locus_tag="RH058_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV3138221.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            674..937
                     /gene="napD"
                     /locus_tag="RH058_002648"
     CDS             674..937
                     /gene="napD"
                     /locus_tag="RH058_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV3138222.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            934..3420
                     /gene="napA"
                     /locus_tag="RH058_002649"
     CDS             934..3420
                     /gene="napA"
                     /locus_tag="RH058_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDV3138223.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3427..4122
                     /gene="napG"
                     /locus_tag="RH058_002650"
     CDS             3427..4122
                     /gene="napG"
                     /locus_tag="RH058_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV3138224.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4109..4972
                     /gene="napH"
                     /locus_tag="RH058_002651"
     CDS             4109..4972
                     /gene="napH"
                     /locus_tag="RH058_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV3138225.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGMLIWEWI
                     NPVSLMGRSLVMGFGSGSLLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4969..5418
                     /gene="napB"
                     /locus_tag="RH058_002652"
     CDS             4969..5418
                     /gene="napB"
                     /locus_tag="RH058_002652"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV3138226.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5428..6030
                     /gene="napC"
                     /locus_tag="RH058_002653"
     CDS             5428..6030
                     /gene="napC"
                     /locus_tag="RH058_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV3138227.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6049..6666
                     /gene="ccmA"
                     /locus_tag="RH058_002654"
     CDS             6049..6666
                     /gene="ccmA"
                     /locus_tag="RH058_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV3138228.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6663..7325
                     /gene="ccmB"
                     /locus_tag="RH058_002655"
     CDS             6663..7325
                     /gene="ccmB"
                     /locus_tag="RH058_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV3138229.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7367..8104
                     /gene="ccmC"
                     /locus_tag="RH058_002656"
     CDS             7367..8104
                     /gene="ccmC"
                     /locus_tag="RH058_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV3138230.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8101..8310
                     /gene="ccmD"
                     /locus_tag="RH058_002657"
     CDS             8101..8310
                     /gene="ccmD"
                     /locus_tag="RH058_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV3138231.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8307..8786
                     /gene="ccmE"
                     /locus_tag="RH058_002658"
     CDS             8307..8786
                     /gene="ccmE"
                     /locus_tag="RH058_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV3138232.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8783..10726
                     /gene="ccmF"
                     /locus_tag="RH058_002659"
     CDS             8783..10726
                     /gene="ccmF"
                     /locus_tag="RH058_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV3138233.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10723..11280
                     /gene="dsbE"
                     /locus_tag="RH058_002660"
     CDS             10723..11280
                     /gene="dsbE"
                     /locus_tag="RH058_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV3138234.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11277..12329
                     /gene="ccmH"
                     /locus_tag="RH058_002661"
     CDS             11277..12329
                     /gene="ccmH"
                     /locus_tag="RH058_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV3138235.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTHSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12364..13011)
                     /gene="narP"
                     /locus_tag="RH058_002662"
     CDS             complement(12364..13011)
                     /gene="narP"
                     /locus_tag="RH058_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV3138236.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13331..15922
                     /gene="yejO"
                     /locus_tag="RH058_002663"
                     /pseudo
     CDS             13331..15922
                     /gene="yejO"
                     /locus_tag="RH058_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16026..16102)
                     /locus_tag="RH058_002664"
     tRNA            complement(16026..16102)
                     /locus_tag="RH058_002664"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16066..16068),aa:Pro,seq:ggg)
     gene            complement(16177..17937)
                     /gene="pbgA"
                     /locus_tag="RH058_002665"
     CDS             complement(16177..17937)
                     /gene="pbgA"
                     /locus_tag="RH058_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV3138237.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17957..18184)
                     /locus_tag="RH058_002666"
     CDS             complement(17957..18184)
                     /locus_tag="RH058_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV3138238.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18366..19373
                     /gene="yejK"
                     /locus_tag="RH058_002667"
     CDS             18366..19373
                     /gene="yejK"
                     /locus_tag="RH058_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV3138239.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19511..19795)
                     /gene="rplY"
                     /locus_tag="RH058_002668"
     CDS             complement(19511..19795)
                     /gene="rplY"
                     /locus_tag="RH058_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV3138240.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19920..21680)
                     /locus_tag="RH058_002669"
     CDS             complement(19920..21680)
                     /locus_tag="RH058_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3138241.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRLTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21829..22524
                     /gene="rsuA"
                     /locus_tag="RH058_002670"
     CDS             21829..22524
                     /gene="rsuA"
                     /locus_tag="RH058_002670"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV3138242.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22552..23742
                     /gene="bcr"
                     /locus_tag="RH058_002671"
     CDS             22552..23742
                     /gene="bcr"
                     /locus_tag="RH058_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV3138243.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAASAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
BASE COUNT         5353 a         5883 c         6742 g         5825 t
ORIGIN      
        1 gtgagcaaaa gcccgttttt gccacgcttc ccgccactct tttgatcctg ctacaggttt
       61 taccccgatc ggggtatgca tctttgacac atcctttaat atcttagcgg ctataaaaat
      121 ggcttattaa ttatgcggtt tatttggccg ctctcaattt tcagagcgcg ttaatgatgg
      181 aaggtcaatg tgaagattga tgcatcccgt cggggcatac tcactggtcg ctggcgcaaa
      241 gccagtaacg gtatccgtcc gccttggtcc ggtgatgaat ctcattttct gacccattgc
      301 acccgttgcg acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg cggcgcgggc
      361 ggctatccga gcgttaattt caaaaataac gagtgcagct tctgttacgc ctgcgcgcag
      421 gcttgccccg aatcgctatt ttctccgcgc cacaccaggg cctgggattt gcagttcacc
      481 attggggacg cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca ggatagctgt
      541 gagccaatgg caattatctt tcgcccgacg ctctccggga tctaccagcc gcaacttaat
      601 agccaacttt gcaacggatg tggagcatgt gcagccagct gtccggtatc agccataact
      661 gcggagtatc tccatgcaca ctaactggca agtttgcagc ctggtcgtgc aggccaaaag
      721 cgaacgaatt tcagacatca gcacccaact gaacgccttt cccggctgtg aagttgctgt
      781 cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa gcagaagaca gcgaaacgct
      841 gatccaaacc attgagtcag tacgcaacgt agagggcgtg ctggcggtgt cgctggttta
      901 tcaccagcag gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg tagctttatg
      961 aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc gggcgttgcc
     1021 cgcgccgttg ttggtcagca ggaagccatt aaatgggata aagcgccgtg ccgtttctgc
     1081 ggtactggtt gcggcgttct ggtcggaacg cagcaggggc gtgtggtggc ctgtcagggc
     1141 gacccggacg caccggttaa ccgtggcctg aactgcatta agggctattt cctgcccaag
     1201 atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa cggtaaatat
     1261 gacaaagaag gcgaatttac cccaatcacc tgggatcagg ccttcgatgt gatggaagag
     1321 aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga tcggtatgtt cggttctggt
     1381 cagtggacta tctgggaagg ttatgccgcg tccaagctgt tcaaagcggg cttccgttcg
     1441 aacaacatcg acccgaacgc acgtcactgt atggcgtcgg cagtagttgg ctttatgcgt
     1501 acctttggta tggatgagcc gatgggctgc tatgacgaca tcgagcaggc tgacgcgttt
     1561 gtgctgtggg gcgctaacat ggcggagatg cacccgatcc tctggtcacg catcactaac
     1621 cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt ctacctacca gcatcgtagc
     1681 ttcgagctgg cggataacgg catcatcttt acgccacagt ctgacctggt gatcctgaac
     1741 tacatcgcca actatatcat tcaaaacaat gcgataaatc aggacttctt cagcaagcac
     1801 gttaacctgc gcaaaggggc gacggacatc ggctacggtt tacgtccgac ccatccgctg
     1861 gaaaaagcag cgaagaatcc gggttctgac gcctccgaac cgatgagctt tgaagattac
     1921 aaagccttcg ttgccgagta tacgctggaa aaaactgccg aaatgaccgg cgtgccgaaa
     1981 gaccagttag aacaactggc gcagctgtat gccgatccga acaagaaagt catctcctac
     2041 tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg ccaacaacct ggtctacaac
     2101 ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg gtccgttctc cctgaccggg
     2161 cagccttccg cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg tctgcctgcg
     2221 gacatggtgg tgactaacga gaaacaccgt gatatttgcg agaagaagtg gaatatcccg
     2281 agcggcacca ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg tgcactgaaa
     2341 gacggcaagc tgaatgttta ctggaccatg tgtaccaaca acatgcaggc cgggccgaac
     2401 attaatgaag agcgtatgcc gggctggcgc gatccgcgca acttcatcat cgtctccgat
     2461 ccgtatccga cagtcagtgc gctggccgcc gacttgatcc tgccgaccgc aatgtgggta
     2521 gagaaagagg gcgcttacgg taacgccgaa cgccgtactc agttctggcg tcagcaggta
     2581 caggcgccag gcgaagcgaa atcggatctc tggcagttag tccagttctc ccgccgcttc
     2641 aaaactgaag aagtatggcc ggaagagctg ctggcgaaga aaccggaact gcgtggcaaa
     2701 acgctgtacg aagttctgta tgccaccccc gaagtgagca aataccctgt atccgaactg
     2761 gcggaagatc agctgaacga tgaatcccgc gagctgggct tctatctgca aaaagggctg
     2821 ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg atctggcacc gttcgatgac
     2881 taccacaaag cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac gcagtggcgt
     2941 tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt ctacggtaaa
     3001 ccggatggca aagcggtgat cttcgcactg ccgttcgaac cggcggcgga agcaccggat
     3061 gaagagtacg acctgtggct ctctaccgga cgcgttctgg agcactggca caccggcagt
     3121 atgactcgcc gtgtgccgga actgcaccgc gccttcccgg aagcggtctt gtttattcac
     3181 ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca aagtgaaagt ggtttctcgc
     3241 cgtggcgaag tgatctcgat tgttgaaacg cgcggtcgta accgtccgcc acagggcctg
     3301 gtgtacatgc cgttcttcga cgccgcacag ctggttaaca aactgacgct ggatgcgacc
     3361 gatccgctct cgaaagagac ggacttcaag aagtgcgcag tcaaactgga gaaggtgtaa
     3421 gccattatgt cccggtcagc gaaacctcaa aatggtcgcc gccgctttct gcgcgatgtc
     3481 gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc tggggttaca acagcaaacc
     3541 gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa cgcctttgcc
     3601 agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac cttaaaactg
     3661 gcgacgctgg cctctggtct gtcggcgggc acgccatatt ttgtcgcacg ggatattcct
     3721 tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc caagcggtgc gctggatcgt
     3781 gagattgaat cgatcgacga cgcgcggatg gggctggcgg tactggtgga ccaggaaaac
     3841 tgtctcaact ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc gaaaattgat
     3901 gaggccatca ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc ccgctttctg
     3961 ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt gcgaaaaagt atgcgtgctg
     4021 gaacaaccgg caatcaaggt gttaccgctg tcactggcga aaggggagtt aggtcaccat
     4081 taccgcttcg gttggctgga ggggaacaat ggcaaatcgt aaacgtgacg ccgggcgcga
     4141 ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac gtcgcctttg
     4201 tcagttcttc gtgctgggga tgtttttgag cggtccgtgg tttggtgtgt ggatcttaca
     4261 cggtaactac agcagtagcc tgttattcga caccgttccg ctgaccgatc cgctgatgac
     4321 gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg gcgttgaccg gtgcggtgat
     4381 tatcaccgtg ctctacgccc tggcaggaaa gcgattattt tgcagctggg tctgcccgct
     4441 gaacccgatt accgacttag caaattggtt acgcaggcgc tttgacctca atcagtctgc
     4501 gacgatccct cgccacattc gctacgtgct gttggtggtc attctgctgg gatcggcctt
     4561 aaccggcatg ctcatctggg aatggatcaa ccccgtttcc ctgatggggc gtagcctggt
     4621 aatgggcttc ggcagcggct cgctgcttat tctcgcgctg tttttatttg atctactggt
     4681 cgttgaacac ggctggtgcg ggcacatttg tccggttggc gcgctgtatg gcgtgctggg
     4741 tagcaaaggt gtgattaccg ttgcggccac cgatcggcag aaatgtaacc gctgtatgga
     4801 ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc ccggtgctgg atgaacaaag
     4861 cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg atgtctgttc
     4921 tgaggatgta tttacaataa ctacacgatg gagttcggga gcgaaatcat gaaaagccat
     4981 gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt aagcggcgcg
     5041 gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg aagtatccgg gacacaggaa
     5101 ggggccattc ggatgccaaa agagcaggaa cggatgccgc tgaactatgt gaatcagccg
     5161 ccaatgatcc cgcatagcgt tgaaggttat caggtaacga ccaataccaa ccgctgcttg
     5221 cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc cgcgtatcag tcctactcac
     5281 tttatggaca gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta tttctgtctg
     5341 caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt taccccatca
     5401 aaaggttacg ggaaataaga ggttattatg ggaaattctg accgtaagcc tggtctgatt
     5461 aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg cgctggggac gctgctgttg
     5521 atcggttttg ttggcggcat cgtcttctgg ggtggcttta acaccgggat ggaaaaagcc
     5581 aataccgaag agttctgcat tagctgccac gaaatgcgca acacggtgta tcaggaatac
     5641 atggattccg tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc ggattgtcac
     5701 gttccgcacg agtttgtgcc gaagatgata cgcaagctca aagcaagtaa agagctgtat
     5761 ggtaaaattt ttggcgttat tgacacgccg cagaaatttg aagctcatcg tctgacgatg
     5821 gcacagaatg agtggcggcg catgaaggac aataactcgc aggagtgccg taactgtcac
     5881 aacttcgagt atatggatac aaccgcccag aaatcggttg ccgcgaagat gcatgaccag
     5941 gcggtgaaag atgggcaaac ctgtattgat tgccataaag ggatagcgca caagctgccc
     6001 gatatgcgtg aagtcgagcc aggtttttaa cagggttatt gcgtgggtat gcttgaagcc
     6061 agagagttac tttgtgagcg ggatgaacga accttattta gtggcttgtc atttacgctg
     6121 aacgcaggag agtgggtaca aatcaccggt agcaacggcg cggggaagac aacgcttctc
     6181 cgtttgctga cggggttgtc tcgccctgac gcaggcgagg ttctctggca agggcagccc
     6241 ttgcatcagg tacgcgacag ctaccatcaa aacctgttat ggataggcca tcagccgggg
     6301 atcaaaaccc ggctgacggc gttagaaaat ctgcactttt atcatcgcga tggcgatacc
     6361 gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg gattcgaaga tattcctgta
     6421 aatcagctct cggccgggca acaacgccgc gtcgctttag cgcgtctgtg gctgacccgt
     6481 gccacgttat ggatcctcga cgagcctttt accgcgattg acgttaacgg cgtcgatcgt
     6541 ctgacccagc gtatggcgca gcatacggag caggggggga ttgtgattct gactacccac
     6601 cagccgctca acgttgctga aagtaaaatt cgccgcattt cactgacgca aacgagggcc
     6661 gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc catagcgccg
     6721 aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac cctttttccg ctcagtatcg
     6781 gtccggagcc gcaactgctg gcgcgtattg caccgggcat tatctgggtt gctgcgctgc
     6841 tttcatcctt gctggcgctg gaacgactgt tccgtgacga tttgcaggac ggcagtcttg
     6901 aacaattgat gttgttgccg ttacccttgc ccgccgttgt gctggcgaag gtgatggcgc
     6961 actggatggt aaccggtctg ccgttactca tcctttcgcc actggtagca atgctactgg
     7021 gaatggatgt ttatggctgg caagtgatgg cgctgacgct tctgctggga acgcctacgc
     7081 ttggctttct cggtgcaccg ggtgtggcgt tgacagtggg acttaagcgc ggtggtgtgc
     7141 tgctcagcat actggtgtta ccgctgacta tcccattact catctttgcc accgccgcga
     7201 tggacgcggc ttccatgcat ttgcccgttg acgggtatct ggcaatttta ggcgcgttgc
     7261 tggcaggcac cgcgacatta agtccttttg cgacggcggc agcgttacga atcagcattc
     7321 aataacgctg gattactgat ttttgttcga gtctggtatc gaaactatgt ggaaaacact
     7381 gcatcaactg gcgatccctc cacggctgta tcaaatctgt ggctggttta taccgtggct
     7441 ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc tggggattcg gctttgctcc
     7501 ggctgattat cagcagggaa atagctaccg cattatctac ctgcatgtgc ctgcggcgat
     7561 ctggtcgatg ggcatttatg catcaatggc ggtggcggct tttattggcc ttgtctggca
     7621 gatgaaaatg gccaacctgg cggtggcggc gatggccccc attggtgccg tgtttacctt
     7681 tattgccctg gttaccggct ctgcatgggg aaaaccgatg tggggcacct ggtgggtatg
     7741 ggatgcacgt ctgacttctg aactggtgct gctgtttttg tatgtgggtg tgattgccct
     7801 gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc tggtgctgat
     7861 tggcgtggtg aatctgccga ttattcatta ctccgtggag tggtggaaca ccctgcatca
     7921 gggatcaacg cggatgcagc aaagtatcga tccggcgatg cgttcgccgc tgcgctggtc
     7981 gatttttggc ttcctgctgc tgtctgccac gctgacgctg atgcggatgc gtaatttgat
     8041 attgctgatg gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa gaggccgtaa
     8101 atgacccctg catttgcttc ctggaatgaa tttttcgcaa tgggcggtta cgcctttttt
     8161 gtctggctgg cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt gcactcggtg
     8221 atgcagcatc gcgcaattct gcgtggtgtg gcgcaacagc gggcgcgtga ggcgcgttta
     8281 cgtgctgcgc aacagcagga ggctgcatga atattcgccg taaaaaccgc ttgtggattg
     8341 cctgtgccgt gttggcaggg ctggcgctga ctatcggtct ggtgctatat gcgctgcgct
     8401 cgaatatcga tctcttttat acgccggggg aaattctcta cggcaagcgt gaaactcagc
     8461 aaatgccgga agtcgggcag cgtctgcgcg tcggcgggat ggtgatgccg ggtagcgtgc
     8521 agcgcgatcc caattcgcta aaagtaactt tcaccattta cgacgctgaa ggttcagtag
     8581 atgtctctta cgaaggcatt ttgccggatc tgttccgtga agggcagggc gttgtggtgc
     8641 agggcgaact ggaaaaaggc aatcatatcc tcgcaaaaga agtgctggcg aaacacgacg
     8701 aaaactacac gccgccagaa gttgagaaag cgatggaagc caatcaccgt cgcccggcga
     8761 gtgtttataa ggacccagca tcatgatgcc ggaaattggt aacgggctgc tgtgtctggc
     8821 gctaggaatt gcgctgctgc tgtccgtgta tccgctatgg ggcgtagcgc gcggagatgc
     8881 gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg tttatgtctg tggctggcgc
     8941 atttctggta ctggtcaatg ccttcgtggt caacgacttc accgtcacct atgttgccag
     9001 caactccaat acccagcttc cggtgtggta tcgcgtggcg gctacctggg gcgcgcatga
     9061 aggctcgctc ctgctgtggg tgctgctgat gagcggctgg acctttgcgg tagcgatttt
     9121 tagtcagcgt attccgctgg atattgtggc ccgcgtactg gcgataatgg ggatggtcag
     9181 cgtcggcttt ttgctgttca ttctctttac ctctaacccg ttctcacgca cgttgccgaa
     9241 cttcccgatt gaagggcgcg atcttaaccc gctgttacag gatccggggc tgatcttcca
     9301 tccgcctctg ctctatatgg ggtacgtggg gttctcggtg gcgtttgctt ttgccattgc
     9361 ttctttgttg agcgggcgtc tggacagcac ttatgcgcgt tttactcgtc cgtggacgct
     9421 ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt tccgcatggg cctattacga
     9481 actcggctgg ggcggctggt ggttctggga tccggtagaa aacgcctcgt ttatgccgtg
     9541 gctggtgggg actgcgctga tgcactcact ggcggtcact gaacaacgcg ccagcttcaa
     9601 agcgtggaca ttactgctgg caatcagtgc cttctcgttg tgtctgctgg ggactttcct
     9661 ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc tctgatccgg cacgcggtat
     9721 gtttatcctc gcctttatgg tactggtgat tggtggttcg ctgctgctgt ttgccgcgcg
     9781 tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg tcgcgggaat ctctgctgtt
     9841 agcgaacaac gttttgctgg tcgccgcgat gctggtggta ttactgggga cgctgctgcc
     9901 gctggtgcac aagcaactgg gactgggcag tatttcgatt ggcgaaccgt tcttcaacac
     9961 catgtttacc tggctgatgg tgccgtttgc gctgctgctt ggtgtcggtc ctctggtgcg
    10021 ctgggggcgc gatcgcccac gtaaaatccg caatttattg attatcgcct tcatctcaac
    10081 gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc aaagttgtgg cgatgacggt
    10141 gctcggcctg gcaatggcct gctggattgc tgtgctggca attgcggaag ctgcgctgcg
    10201 tatttcacgt ggcacgaaaa ccaccttcag ttattgggga atggtggcgg ctcacctggg
    10261 gctggcagtg acaattgttg gtattgcctt tagccagaac tatagcgttg agcgtgatgt
    10321 gcgcatgaag tccggcgata gcgtcgatat tcatgaatat cgcttcacct tccgtgatgt
    10381 caaagaggtg actggcccga actggcgtgg cggtgtggcg actatcggcg taacgcgcga
    10441 tggcaagccg gaaacggtgc tgtatgcgga aaaacgttat tacaacactg ccgggtcgat
    10501 gatgaccgaa gcggcaattg acggcggcat cacgcgtgac ctgtacgccg cgctcggtga
    10561 agagctggaa aacggcgcgt gggccgtgcg tctttactac aaaccatttg ttcgctggat
    10621 ttgggcgggc gggctgatga tggcgttggg cggactactg tgtctgtttg atcctcgcta
    10681 tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggcc gtatgaagcg caaagtattg
    10741 ttaattccgt tgattatctt cctggcgatt gccgcggcgc tgctgtggca gctggcgcgt
    10801 aatgccgaag gggatgatcc gaccaatctg gaatcggcgc tcattggcaa gcctgtgccg
    10861 aagtttcgtc tcgaatcact ggacaatccg gggcagtttt atcaggcgga tgtgctgact
    10921 cagggcaaac cagtgctgct taacgtctgg gcgacctggt gtccgacctg ccgtgcggaa
    10981 catcaatatc tgaatcagct ttcagcgcag ggcattcgcg tggtcggcat gaactataaa
    11041 gacgatcgcc agaaggcaat cagctggctg aaagagctgg gcaatcctta cgcgctaagc
    11101 ctgtttgatg gcgacggcat gttagggctg gatctcggtg tctatggcgc gccagaaacc
    11161 ttccttattg acggcaacgg tatcattcgc tatcgccatg cgggcgatct caatcctcgc
    11221 gtctgggaag aagagatcaa gccgctgtgg gagaaataca gtaaggaggc cgcacaatga
    11281 ggtttttatt gggcgtgctg atgctgatga tctccggctc agcactggcg accatcgacg
    11341 tgttgcagtt taaagatgaa gcacaggaac aacagttccg tcagctcact gaagaactgc
    11401 gctgcccgaa atgccagaac aacagcattg ccgattccaa ctcgatgatt gccaccgacc
    11461 tgcgtcagaa agtgtatgaa ctgatgcagg aaggtaaaag taaaaaagag attgtcgatt
    11521 atatggtggc gcgttacggc aacttcgtca cttacgatcc gccgttaacg ccgctgaccg
    11581 tgctgctgtg ggtgcttccg gtagtggcta ttggcattgg cggttgggtc atatacgccc
    11641 gctcgcggcg tcgggtacgc gtagtgccgg aagcgtttcc tgaacaaagc gtgcaggaag
    11701 gtaagcgtgc cggatatatt gtttatttgc cgggcattgt ggtggcgtta attgtggctg
    11761 gcgtcagcta ctaccagacc ggaaattatc agcaggtgaa aatctggcag caggccacgg
    11821 cacaggctcc ggcgttgctg gacagggcgc tggatccgaa agccgatccg ctcaacgaag
    11881 aagagatgtc gcgtcttgcg ctggggatgc gtactcaact gcaaaaaaat ccgggagata
    11941 tagaaggctg gattatgttg ggccgcgttg gcatggcgct gggtaacgct agtatcgcca
    12001 ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa cagcgatgcc gcactgggtt
    12061 atgctgaagc gttgacacat tcatctgatc ccaacgacaa ccgcctcggt ggtgaactgc
    12121 tgcgccagtt ggtgagaagc gaccacagca atatccgtgt gttaagcatg tatgcgttta
    12181 atgcctttga gcagcagcga tttggcgaag ccgttgccgc gtgggagatg atgttgaaac
    12241 tcttacctgc caacgatact cgccgtgcgg tgattgaacg tagtatcgcg caggcgatgc
    12301 aacatttgtc gccgcaggag agtaaataaa aagaggcccg atggtgatgc catcgggcta
    12361 tttttattgt gccccgcgtt gttgcaggaa cagaatggtg gctgccacgc gtgagcggac
    12421 attgagtttg cgcagcagat tgcgaatatg tacttttact gtctgctctg aaatattcaa
    12481 caccgaggca atctgtttat ttgacagccc ctgtgccagc tcgtgcagaa catccagctc
    12541 gcgctccgtc agcacgctga agggatcttc ttccgcgcca aacatttcac gttcacgtaa
    12601 gtactgattg acgcgttcgc taaagacttt gctgcctttc gctccagcac gaattgcttc
    12661 cagcaatact tccgggtcgc tgtctttcaa cagataaccg tctgcgcctg cgtctatcag
    12721 cgcaaagaca tcgctggagg catctgatac ggtcaggata ataatttgcg cggtaacgcc
    12781 atccctgcgc aaggcattga gggtatccag gccactcata cctttcatat tgagatccag
    12841 caagatcacg tcgatatcca gtctattcgc cagatcgatc gcgctcgcgc cgtcgcccgc
    12901 ttcggcgacc acttcaaagc caggatcaag ctccagtaac tgacgaacac cgcgtcgcat
    12961 aagtggatga tcatccacaa tcatcacctg aaaaggcgtt gcttcaggca tagtagtctc
    13021 ctgaggtttt attagaatca ttatgtttga gcgtagcgtt ggtaacgcta ccaggcgcgt
    13081 gaatgttacc ccattttatg tatggacatg tagtgaaaac tacaaccttg tcgttcgctt
    13141 tggcatcatt ttcattataa agacggaatt ttgcatcagc ttctcatcaa gaaaaacaac
    13201 taaaaaatca atcacaatac ttgtgcattt ctttaaacag tttttaaaaa ctgactccgg
    13261 gtatggagct atgggtattt tccgtaccca atgcttttaa cagcaattaa tttcatagga
    13321 tgaaagctca attcatcaat ctggttctgt ttctctttgt cgttccgcaa tatctgttct
    13381 ggtggctaca gcgttatatt cacccatagc attggcatca acagttgagt atggtgagac
    13441 agttgatggt gttgtcctgg aaaaagatat ccagctggtt tatgggaccg ccaataatac
    13501 gaaaatcaat cctggcggag aacagcatat aaaagaattt ggtgtaagta gtaatactga
    13561 aattaaagga gggtatcagt acattgaaat gaatggtacc gcagaatact cagtattaaa
    13621 tgacggttat caaattgttc aaatgggtgg cgcggcaaac cagactaccc tcaataatgg
    13681 tgtgctacag gtttatggcg cagcgaatga tcccacgatt aaaggcgggc gcttaatcgt
    13741 tgaaaaagat gggattaccg tccttgccgc tatcgaaaag ggaggattac tggaggttaa
    13801 agagggggga ttagcgattg cggtagatca gaaagcaggc ggtgctatta aagcaagcac
    13861 gcgggtcatg gaggcattcg gaacaaaccg tctcggtcag ttcgaaatca agaatggtat
    13921 tgctaacaat atgctgttgg aaaacggcgg aagtttgcga gttgaagaaa atgacttcgc
    13981 ttataatact actgtagata gtggcggctt actggaggtt atggatggcg ggactgtaac
    14041 tggcgttgat aaaaaagcag gcggaaaatt aattgtctca acgaatgcgc tggaagtgag
    14101 tggtacaaac agtaaaggcc aatttagtat aaaagatggt gtgtcaaaaa attatgaact
    14161 ggatgatggt tccgggctca ttgttatgga ggacacgcag gccattgata ctatccttga
    14221 tgagcatgcc actatgcaat cgctgggaaa ggatactggt acgaaagtgc aggcaaatgc
    14281 ggtatatgat ctcggtcgat cagatcagaa tggaagtatc acgtattcct caaaagccat
    14341 ctctgaaaat atggttatca acaatggccg cgctaacgtc tgggctggca caatggttaa
    14401 cgtttcagtc agagggaatg atggcattct tgaggtcatg aagccgcaaa taaattatgc
    14461 acccgcaatg ttggtgggta aggtagtggt ttctgagggc gcttctttta gaacgcatgg
    14521 tgccgtggat accagcaaag cggacgtttc gctcgaaaat agtgtatgga ccatcattgc
    14581 cgatatcact acgacgaacc aaaacaccct cctcaactta gccaaccttg cgatgtctga
    14641 cgcaaatgtg attatgatgg atgagccagt gactcgttca tcagtgacgg caagtgcgga
    14701 aaatttcatt acgttgacca ccaataccct gtcgggaaac ggcaattttt atatgcgtac
    14761 cgatatggct aatcatcaga gcgatcagct caacgccacc ggtcaggcaa caggtgattt
    14821 caaaatattc gtgacggaca ccggtgccag cccggcagca ggagatagcc ttacactggt
    14881 aacaacgggc ggcggtgatg ctgcatttac gttgggcaat gccggaggcg ttgttgatat
    14941 cggtacgtat gaatatacct tgctggataa tggcaaccat agctggagtt tggcagagaa
    15001 tcgcgcgcaa attacccctt caaccactga tgtgctgaat atggcggcag cacaaccgct
    15061 ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt ggtagcgtaa aaggcgttaa
    15121 ttacgatacg gcgatgtgga gttcggcaat taacacccgc aacaacgtga ccactgatgc
    15181 gggcgctggc tttgagcaaa cattgaccgg gctgacgctc ggtatcgata gccgtttctc
    15241 ccgtgaagaa accagcacaa ttcgcggctt gttctttggt tactctcatt ctgatattgg
    15301 ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc ctgggggctt atgccggttg
    15361 ggagcatcag aacggtgcct atgttgatgg agtggtgaaa gttgaccgtt ttgccaacac
    15421 catccattgc aagatgagta atggggcaac agcgtttggc gattacaata gtaacggcgc
    15481 gggtgctcat gtcgagagcg ggttccgttg ggttgacgga ttgtggagtg ttagacccta
    15541 tctggccttt accggcttta ccacagatgg tcaggactac acgttatcaa acggcatgcg
    15601 cgcggatgtg ggaaataccc ggatattacg cgctgaagcg ggaacggcgg taagctatca
    15661 catggacctg caaaacggta cgacgctgga accctgactg aaagccgccg tgcgtcagga
    15721 atacgccgat tctaaccagg tgaaagttaa tgacgatggc aaatttaata atgatgtggc
    15781 tggaacccgt ggcgtttatc aggctgggat aaggtcatcg tttaccccga cgttaagcgg
    15841 tcatttgtca gtcagctatg gcaatggcgc aggggtagaa tcgccgtgga atactcaggc
    15901 gggtgtggtc tggacgttct gataacaaaa aataaacagg ctgtgatgtg tcacggtctg
    15961 tttatcgaat taattgcaga tataaaaaaa accaaccgta aaggttggtt ttttcttggg
    16021 atttttggtc ggcacgagag gatttgaacc tccgaccccc gacaccccat gacggtgcgc
    16081 taccaggctg cgctacgtgc cgactcgtgg ctgctaatac taccgttttc cacaccgatt
    16141 gcaagtaaga tattttgcta actgatttat aattaatcag ttagcgataa aacgcttctc
    16201 gtctgtcagc acttgcaata acaaacttaa ctgtggtttt tcatctttca ctctttcacc
    16261 acgtaagttg taagtgcggt atttaccgtt attgttcagc accagcgtct ttttcggggt
    16321 ggtaattgcc agcgtatcgt tatccgctgc agtaacccag taatgacggc gttgagggtt
    16381 gaacaaatcc tgaccttgcg aatattcgct ggcaggtgtg ctgacatgta gcaggcgttg
    16441 catcagcgtc gtcatcagat cggtatgatc agtcagagca ttaatacgct gcgccggcgt
    16501 gcctggccag tgaatcacta atggcacctg cagatgaccg tgggaccagt caaaggtttc
    16561 ttcgtcttcg ctcagcggaa taccccgacc ggcagtgata atcaccaccg tattgtccag
    16621 tttgccagaa tcacgcagtg cattgagcac gcggttgatc tggtcatcga cattgcctgc
    16681 cgcccggcta tatttccgtg caaatgcctg ctgattgctg tcgtcaatgt tagtgccatt
    16741 gaaagagacc cacgagaacc agcggttatc ttcttgtgcg tagcggccca gccagttgat
    16801 ccactgcgtg gcggtctgct cgtcggattg ggtgcgtacg ctcggcatcg agaaatctga
    16861 caacaatgcc tggcgataca gcgggctggt aaagccatct gatgagaata atcccagctg
    16921 atagccttgc tgattaagcg cagtaattaa cgccgcaggg gtacgggtcg acagaatgcc
    16981 gtccatatag ctcggcgaga tgccatagaa caggccaaag atgccgttgt ctgtagtgtt
    17041 gccggagctc atatggcgcg tgaacgaaat attttgctca gcaaaacctg ccagcgcagg
    17101 catctgcttc tcgaagcgtg agtagttcag gccatcgaca gtaatcaaca gcacattctg
    17161 cccggtgccc atatcgcgat agcgcagttc gcttaacgga tactgaacgg aaacggcgtc
    17221 tggattacct tgctcaataa gacggcgttg atactcctgc gcatcaagca gaccatgctt
    17281 ctcaagaaaa cgtcgcgccg tcatcgggta cgaaagcggc aggttagcgc gctgcatggt
    17341 gatcgggcga tagaagttgg catcggccca gatatacacc acatgcgagg cgataaaggc
    17401 gataaataag aatgcggcca gcgggcgcgc gaagcgccga cgacgcgtca ggctgcgcag
    17461 cttttgccag ctccacgtcg caaacaccag ttcaagcaat aaaataaccg gcacgctgat
    17521 gaacatcagc tgccagtcgc gcgccatctc attttcgtct gggttgataa ccagttgcca
    17581 gacgatggga ttaagatgga gatggaaacg agtaaagact tcgctatcga tcagtaatag
    17641 cgtcattccc gccgttgcca gaatgacgga caaaaacctc atcagcctct gggagccgac
    17701 gataaaggtc agcgggaaga ggatcagcaa gtaggtggcg aacaccagga agctgaaatg
    17761 gccgataatg cttacgtagg aataaatgcg accagcaagc gttgtcggcc agtcggcgat
    17821 aaacaggtaa cggctgccaa tgacgagcga aagcagaata ttgaacagtg caaaccagtg
    17881 cccccaactg accatctggg agactttttc acggtagcgc tgacgatgag ttaccataac
    17941 ctgttatctg tttcccttag tgcgctttgt cttcgttgat agaggactgt aaggcgcggg
    18001 caaaagagtt ggcaattgcc tggcgttggg ccggggcaat gctggtattg ataaggttag
    18061 tgaccatgtt gcctaacacc atcagggaaa ggtcggtcgg agccttatgt ttttccagta
    18121 cgttgagcag ctctgcgagc aattgttcaa cctgttcatc actgtagcgg gaaatttgtg
    18181 gcataaatcg aaatcagtta gttgatgaaa gggcaacata ttaccgtagc aacggttttt
    18241 tttctgcttt tttatgcgtt aaatcattct cgttacgctg ccttctgtaa aatgaccgcc
    18301 atcttttatt gcgtcgctta accggcggtg gttgaatacc gcccggtctt aaaggagagt
    18361 ttatcatgag tctggatatc aaccagattg ctctgcacca gcttatcaag cgtgatgagc
    18421 agaaccttga gctggtgttg cgcgattcat tgctggaacc gacagaaacc gtcgttgaga
    18481 tggtggcaga actgcatcgg gtctatagcg ccaaaaataa agcttacggc ttgtttagcg
    18541 aagagagtga actggcgcag acgctgcgct tacagcgtca gggcgaagag gactttctgg
    18601 catttagccg cgcggcaact ggtcgcctgc gtgacgaatt agcgaaatat ccttttgctg
    18661 acggtggatt cgtgctgttt tgccactacc gttatctggc ggtggagtat ctgctggtgg
    18721 cagttctgag caacctgagc agtatgcgcg ttaacgaaaa tctcgacatc aatccaaccc
    18781 attatcttga tatcaatcat gcggatattg ttgcgcgtat cgatttaacc gaatgggaaa
    18841 ccaatccaga gtccacccgc tatctcactt tcctgaaagg gcgagtaggg cgcaaagtgg
    18901 cggatttctt tatggatttc ctcggggcca gcgaagggtt aaacgcgaaa gcgcaaaacc
    18961 gtgggctgtt gcaagcggtt gatgatttca ctgccgaagc acaactggat aaagcagagc
    19021 gacagaacgt gcgccagcag gtttatagct actgtaatga gcagctgcag gcaggcgaag
    19081 agatcgaact ggagtcgctg tcgaaagagc tggcaggcgt cagcgaagtg agtttcacgg
    19141 agtttgcggc tgaaaaaggc tacgaactgg aagagagctt cccggcagac cgtagcacac
    19201 tgcgtcagtt gacgaagttt gccggtagcg gcggtggctt gactattaac tttgatgcga
    19261 tgctgttagg tgaacgcatc ttctgggatc cggcaaccga taccctgacc attaaaggca
    19321 cgccgccgaa tttgcgtgac caattgcagc gccggacgtc tggcggcaat taatcggttt
    19381 tgtgttctga atgccagatg cggcgtgaac gccttatctg gcctgcaaaa aatagcaata
    19441 ccgtaatcag gcataatctg cagatacaaa aaccccgccg gagcggggtt ttttacaact
    19501 tattcagcaa ttaagcgcga acgaagtcga tgtgctgcag cttcggtttg tacgggtgac
    19561 gctgtacgtc ctgagcttta actttgattt ctttaccgtc aacaacgatg gtcagaactt
    19621 cgctgtagaa ttcagcttta gcttgcatgt tcatgacttt gtcgtgatcc agctcgatag
    19681 ccagcggcgc ttctttgcca ccgtagatga ttgccgggaa cttgttagcg gcacgcaggc
    19741 ggcggctcgc acccttaccc tgctctttac gtacttctgc gttgatagta aacatttctt
    19801 tctctcttaa attaatcctg ctacaggcga cccagcaaca ggtaagtgat ctgctttgcg
    19861 tatgcaaaag cgggcgagat tctatactca atcgctttat acatcaatca aaactaccat
    19921 taaccgcgta attcgtgcgc ccgacgaaaa cgaccttcgt aatcgaacac tttttcacgc
    19981 acttgccagt actgaccttt catgcgggca acgacaaaat ccgggtgtcg caataaggct
    20041 tgctgggcga ggatatcggc ggcggtgatc cagcgcagag ggatgcccgg tgtgcgcgta
    20101 tgcgggcgga taaaaagctg ctcgaaggca gttcgctggg cgggcgtttg cagacggaaa
    20161 cgctcactca catccgcgcc gtcttcatcg taataggtga ttttcaacca ttcgcctttc
    20221 tcgtcgtgcc catgttgcaa agacatgccg ctacagcgta ataccagcgc gtctttcagt
    20281 cgtagcgccg cttttaacat atcgtccgga tcaaccagta cggtgtcaca ttcgcggcag
    20341 cggcgggcgg caatatcatt ttctgcgtta cattgcgggc aatttttaaa acggaaacgg
    20401 aagtcacact gttcgcgatg accgtcgtca tcttcaaacc atccctgaca gcgacgacca
    20461 aagtgttcaa tcaatgtccc gtcggcggtc gttttccccc aaaaggtgtt ggcaaaaccg
    20521 caggcagggc agaaaacctg taccgggacg ttgtcacttt tgcctttcgg tgtaccaact
    20581 tccggcgcgt agagatcgtg aggattaccc gcataatcaa gaattaagca atcagtcttg
    20641 ccaggagcga gacgcagacc gcgcccgaca atttgttggt aaagactcac tgattcggta
    20701 aggcgcagaa tggcgataag atcgacgtgc ggggcgtcaa atccggtggt cagtactgcg
    20761 acgttgacca gatagcgaaa acgctgggct ttaaaatctt caattaacac atcgcgctca
    20821 gcgccggggg tgtcgccagt aatcagtgca gcatcttcgg cgggcagtaa tcccacaatc
    20881 tcttttgcgt gttcgaccgt cgcggcgaaa atcattaccc ctttgcgctt ttcggcgaac
    20941 tccatgatct ggctgatgat atgtggcgta atgcgttgct gttttttcag ctcacggttg
    21001 agatcagctt cgctgaacag cccgttactc tgtgcctgca agcggctgaa atcgtattgt
    21061 actactggca tatccagtcg ttctggcggc gtcagatagc cgtgtttaat catataacgc
    21121 agcggcagct cataaatgca gtcacggaaa agggctttct catcgccgcg taccatgccg
    21181 tgataatgga actggtagat ccagcctttg cccaatcgaa aaggcgtggc agtcagcccc
    21241 agcaggcgta agtggggatt cacttttgtc aggtgagtga ggatttgctg atactggctc
    21301 tcttcatcgt caccaatacg gtgacattca tcgacaatca acagcgaaaa ttcaccctga
    21361 aaggcatcaa gattacgggc gaccgactgc acactgccaa ataccacttt accgtggctc
    21421 tctttacgct ttagcccggc ggcaaaaata tcggcttcca gccccagcgc ctgatacttt
    21481 gcatggtttt gcgccaccag ttctttaacg tgtgccagca ccagtacgcg accacgagcc
    21541 agccgtgcca gttccgctat caccaggctt ttacctgcgc cggtgggcag cacgataacg
    21601 gcaggggttt tatgacgacg aaaatggttg agcgtggcat ccacggcttc ttgctgatat
    21661 gggcgaagtg taaaaatcat ggtctcgcgt aggtagatta ttctgctaat agtatgccac
    21721 ggttcttttt cccttgaggg aagttattag accgctatac tgggcagaca acagttcaga
    21781 ttttcgggtc aatgcccgac ctccgttttt acaggcaaaa tcacacacat gcgacttgat
    21841 aaatttatcg cacagcaact cggcgttagc cgtgctattg ccgggcgtga aatccgcggc
    21901 aatcgtgtca ccgtcgatgg cgaaatcgtc cgtaatgcag cgttcaaact gcttcctgaa
    21961 catgatgtcg cttacgatgg caacccgctg gcgcagcaac atggtccacg ttacttcatg
    22021 ctcaataagc ctcagggcta tgtttgctcc acggacgacc ctgatcaccc aacggtgctc
    22081 tattttctgg atgaaccggt agcctggaaa ctgcacgccg caggacgtct ggatatcgat
    22141 accaccggtc tggtgctgat gactgatgat ggtcagtggt cgcaccgcat tacttctccg
    22201 cgccatcatt gcgagaagac ctatctggtg acactggaat cacctgtagc tgacgatacg
    22261 gcagagcaat ttgccaaagg cgtacagctg cataacgaaa aagatctcac taagcctgcg
    22321 gtgctggaag tgattacccc aacgcaggtt cgtctgacca tcagcgaagg gcgttatcat
    22381 caggtgaaac gcatgttcgc cgctgtgggt aaccatgtgg ttgagctgca tcgtgaacgt
    22441 attggcggta ttacgctgga tgctgattta gcccccggtg aatatcgtcc gttaactgaa
    22501 gaagaaattg ccagcgtcgt ctaacttttc catgactttc aggagcccgt tgtgaccacc
    22561 cgacagcatt cgtcgtttgc tattgttttt atccttggcc tgctggccat gttgatgccg
    22621 ctgtcgattg atatgtatct gcccgcgcta ccggtaattt cagcgcagtt tggcgtacct
    22681 gcgggcagta cgcagatgac cctcagtact tatattctgg gctttgcgtt ggggcagtta
    22741 atctacgggc cgatggcaga cagcttcggg cgtaagccgg tggtgctcgg cggtacgctg
    22801 gtgtttgccg ccgccgcggt ggcgtgtgcg ttggcaaaca ccatcgatca gctgattgtg
    22861 atgcgtttct tccacgggct ggctgcgtct gcggccagcg tggtcattaa cgccctgatg
    22921 cgcgatattt acccgaaaga agagttctcg cggatgatgt cgtttgtcat gctggtgacc
    22981 actattgcac cgctgatggc accgattgtc ggcggttggg tgctggtgtg gctgagctgg
    23041 cattacatct tctggatcct ggcattagcg gcgattctgg cttcggcaat gattttcttc
    23101 ctgattaaag aaaccttacc accggagcgt cgccagcctt ttcacattcg taccactatt
    23161 ggtaactttg cggcgctgtt ccgtcataag cgtgtcctga gttatatgct tgccagtggt
    23221 ttcagctttg ccgggatgtt ctcgttctta agcgccggac cgtttgtcta cattgaaatt
    23281 aaccacgtcg cgccggaaaa ctttggttat tactttgcac taaacattgt tttcctgttc
    23341 gtgatgacca tttttaacag ccgcttcgtc cgccgcattg gcgcgttaaa tatgttccgc
    23401 tcggggttgt ggatacagtt tattatggcg gcgtggatgg tcatcagtgc gctgctgggg
    23461 ctgggattct ggtcgctggt ggttggcgtt gcggcgtttg tgggctgcgt gtcgatggtg
    23521 tcatccaatg cgatggcggt cattcttgat gagtttcccc atatggcggg aacggcatct
    23581 tcgctggcag gaaccttccg ttttggcata ggggcaattg ttggcgcatt gctttctctt
    23641 gcgaccttta actctgcatg gccgatgatt tggtcaattg cattctgcgc aaccagctcc
    23701 attctcttct gtctgtacgc cagtcggccg aaaaaacggt gatctattgc acaacgagga
    23761 agctaaaagg cttcctttgt tgatgcatgt caaccacaaa tct
//
LOCUS       DAPHDX010000070        23405 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPHDX010000070 DAPHDX010000000
VERSION     DAPHDX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.070"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(321..1118)
                     /locus_tag="RH058_002672"
     CDS             complement(321..1118)
                     /locus_tag="RH058_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3138244.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1128..1679)
                     /gene="ybcL"
                     /locus_tag="RH058_002673"
     CDS             complement(1128..1679)
                     /gene="ybcL"
                     /locus_tag="RH058_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDV3138245.1"
                     /translation="MKKPIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1848..2180)
                     /gene="emrE"
                     /locus_tag="RH058_002674"
     CDS             complement(1848..2180)
                     /gene="emrE"
                     /locus_tag="RH058_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDV3138246.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2514..2828)
                     /gene="fliE"
                     /locus_tag="RH058_002675"
     CDS             complement(2514..2828)
                     /gene="fliE"
                     /locus_tag="RH058_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV3138247.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3043..4701
                     /gene="fliF"
                     /locus_tag="RH058_002676"
     CDS             3043..4701
                     /gene="fliF"
                     /locus_tag="RH058_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV3138248.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4694..5689
                     /gene="fliG"
                     /locus_tag="RH058_002677"
     CDS             4694..5689
                     /gene="fliG"
                     /locus_tag="RH058_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV3138249.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5682..6368
                     /gene="fliH"
                     /locus_tag="RH058_002678"
     CDS             5682..6368
                     /gene="fliH"
                     /locus_tag="RH058_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV3138250.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6368..7741
                     /gene="fliI"
                     /locus_tag="RH058_002679"
     CDS             6368..7741
                     /gene="fliI"
                     /locus_tag="RH058_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV3138251.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7760..8203
                     /gene="fliJ"
                     /locus_tag="RH058_002680"
     CDS             7760..8203
                     /gene="fliJ"
                     /locus_tag="RH058_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV3138252.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8200..9327
                     /gene="fliK"
                     /locus_tag="RH058_002681"
     CDS             8200..9327
                     /gene="fliK"
                     /locus_tag="RH058_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV3138253.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9432..9896
                     /gene="fliL"
                     /locus_tag="RH058_002682"
     CDS             9432..9896
                     /gene="fliL"
                     /locus_tag="RH058_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV3138254.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9901..10905
                     /gene="fliM"
                     /locus_tag="RH058_002683"
     CDS             9901..10905
                     /gene="fliM"
                     /locus_tag="RH058_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV3138255.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10902..11315
                     /gene="fliN"
                     /locus_tag="RH058_002684"
     CDS             10902..11315
                     /gene="fliN"
                     /locus_tag="RH058_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV3138256.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11318..11683
                     /gene="fliO"
                     /locus_tag="RH058_002685"
     CDS             11318..11683
                     /gene="fliO"
                     /locus_tag="RH058_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV3138257.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11683..12420
                     /gene="fliP"
                     /locus_tag="RH058_002686"
     CDS             11683..12420
                     /gene="fliP"
                     /locus_tag="RH058_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV3138258.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVSSLAQSFYS"
     gene            12430..12699
                     /gene="fliQ"
                     /locus_tag="RH058_002687"
     CDS             12430..12699
                     /gene="fliQ"
                     /locus_tag="RH058_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV3138259.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12708..13493
                     /gene="fliR"
                     /locus_tag="RH058_002688"
     CDS             12708..13493
                     /gene="fliR"
                     /locus_tag="RH058_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV3138260.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGVALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13783..14406
                     /gene="rcsA"
                     /locus_tag="RH058_002689"
     CDS             13783..14406
                     /gene="rcsA"
                     /locus_tag="RH058_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV3138261.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14450..14638)
                     /gene="dsrB"
                     /locus_tag="RH058_002690"
     CDS             complement(14450..14638)
                     /gene="dsrB"
                     /locus_tag="RH058_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV3138262.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14801..15028
                     /gene="yodD"
                     /locus_tag="RH058_002691"
     CDS             14801..15028
                     /gene="yodD"
                     /locus_tag="RH058_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV3138263.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15326..16141
                     /locus_tag="RH058_002692"
     CDS             15326..16141
                     /locus_tag="RH058_002692"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV3138264.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIMKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16138..17832)
                     /gene="yedQ"
                     /locus_tag="RH058_002693"
     CDS             complement(16138..17832)
                     /gene="yedQ"
                     /locus_tag="RH058_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDV3138265.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     LLQWQAWHDTLTRLYNSGALFEKARSLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(18003..18185)
                     /locus_tag="RH058_002694"
     CDS             complement(18003..18185)
                     /locus_tag="RH058_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV3138266.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18264..19181)
                     /locus_tag="RH058_002695"
     CDS             complement(18264..19181)
                     /locus_tag="RH058_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV3138267.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19354..20274
                     /gene="yedA"
                     /locus_tag="RH058_002696"
     CDS             19354..20274
                     /gene="yedA"
                     /locus_tag="RH058_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV3138268.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20263..20733)
                     /gene="vsr"
                     /locus_tag="RH058_002697"
     CDS             complement(20263..20733)
                     /gene="vsr"
                     /locus_tag="RH058_002697"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV3138269.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGLGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGCRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20714..22132)
                     /gene="dcm"
                     /locus_tag="RH058_002698"
     CDS             complement(20714..22132)
                     /gene="dcm"
                     /locus_tag="RH058_002698"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV3138270.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKDGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AIFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22199..22894)
                     /locus_tag="RH058_002699"
     CDS             complement(22199..22894)
                     /locus_tag="RH058_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDV3138271.1"
                     /translation="MDLQHWQTQFENWLKNHHQHQDAAHDVYHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22934..23291)
                     /locus_tag="RH058_002700"
                     /pseudo
     CDS             complement(22934..23291)
                     /locus_tag="RH058_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         5782 a         6116 c         5876 g         5631 t
ORIGIN      
        1 taacgtataa actagattta ataacaacga tatccgtacc tatccggata tctaatgtca
       61 taagcatccc tttaattatc ccctcaacct cttgtaactt tcccactctt gaactccaca
      121 tagtccaaac gccggatgca aaaaaatcac acgtgcattt ctgaaattgt gatggaggaa
      181 gatagtatca attatatgtg cttataaacg tccccgatta gcgcagaagt caccggattg
      241 ttcaggctcc aataggttga gtatgccgta tttattttcc aaaataaaaa tcgatgctgt
      301 tgctccttca taaaatactt ttactctctg gacgcttttc ttagctctct tggtgttatc
      361 ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac acttgtaacc attttccctg
      421 gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag acaacgcatt atttaacctc
      481 acgtcgtcca atatactttg aaaacttact ccctcgcttg ccagacggcg atgcaatgtg
      541 gaaacagaaa tgtagagata tctcgctacc ttgtttgctg tccattttgt tccaggttcg
      601 gatagcatta ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa
      661 caattaacat gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt
      721 tgcgttgaga agcctcggga aatagatgtt tttagttgct cccaacaata tattaactcg
      781 ggattctgag gtaagcagaa acctgtcgtg ttacgaattt gatcggttat cgcataaaga
      841 ttttggaact tttcaattag atcaatggga taataaagca tttctgcaag ataaagacct
      901 gcttctgggt aattcgcaat ataaaattca tatccacagg gaaataatat tatctgatta
      961 ttatcaacag ttagactatg cgtctcccaa ttgataactt tttttccctg acggatacga
     1021 cataaagctg gcataagagg cttaaccctg tgaatctcat gatgtttatg catccgtatt
     1081 tcttcaatct ttaggttagt cctatctttt gccagcatac tcttatccta ctttacctca
     1141 taaacgggta ttatctcagc ggttgcaatt ttattggcat taagcatata accaactaac
     1201 gctccgctgg aattagaatc cacaggaatc ttatcagttt ttagagccca tattttaaac
     1261 tggtaatgat ggggtttatc tcctttggga ggacatgctc caccaaatcc cgcatagcca
     1321 aaatcatttc ggccttggac agcaccagtc ggaagttttg taccatcacg tcttcctgca
     1381 tcagcgggca aatatgttac cgttgctgga atattagcaa cagtccagtg ccaccatccg
     1441 ctgcctgtag gcgcatctgg atcatacaca gttacggcaa agcttttggt gccttctgga
     1501 gcaccagacc aggttaatga gggcgatgta ttacccccct cacacccaaa tccagaaaat
     1561 acatgagacg ttgtaagttg ctctcctgtt tttatttcat tactagtgac ctgaaatgct
     1621 gcggcctgcg cagaaaatgt tatgaatgcc aatacacttg aaacgatagg ttttttcata
     1681 aaaacctctt tgttgtgacc tatcgttatt ttatttgatg tgcattgatc tcattatgca
     1741 aaaaggcgca atgatcgcgc aaatgcaatc ataattataa agttgccccg aacattagcc
     1801 ataatatgca caaatcagat tcctgttatc tcaaggatga ttatttttta atgcggtgcg
     1861 cttcgtgaca acaagttaat gactaacaca ccggcacaaa tcaacatcat gcctatgatg
     1921 gctggcaagt ccagtcgttg gccaaaaaat ccccaggaaa gcaaactaat cagaacgata
     1981 ccgactcctg accagatagc ataagcaatc cctgtaggta tataagccag tgtctgagct
     2041 aataaccaaa atgatgcaca atagcaaata attgtgccaa cagagggcca taaccgtgta
     2101 aacccctctg aaaacttcat taaggttgta ccaatgacct ctgcaagtat tgcgccacca
     2161 agataaatat aagggttcat agcatattct ttcctgttca tactggagag aattgtacta
     2221 cagtttgaac acaactcacc gttttcatct tggtgtatgt gtatccaggg ggctctggcg
     2281 tggcaagaga taaaggcagg aacaataatg atcagaagga accccaggct aacaaaagag
     2341 ttcactcttc ctatgagcaa agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga
     2401 tggcagaaaa ctttcaggat caggtatcca ttttaaacca gaaattgagt gagtttgccc
     2461 catccatgcc ccaagcggtg agattggaag taattaacaa caggttacaa aacctacacc
     2521 tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt
     2581 tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttaacgc cacgccgggt
     2641 tcaccgaggg tgaatttttc tgcctgcgtg cgggcagctg tttgtgtatc gctaatgcga
     2701 tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag tgaatcctgc
     2761 gcacgcgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc
     2821 gctgacattt tcgtctcctg gataatttct ggtagcaaag cctaccagta agtcaataag
     2881 acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg cggatagaaa aaaacgaaag
     2941 cacaaataat tgcagcgtca atttttcgag tttgctgacc cgggagtgag tcttgttcca
     3001 ctttgccaat aacgccgtcc ataatcagcc acgaggtgcg cgatgaatgc gactgcagcc
     3061 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
     3121 gttgccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc
     3181 gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa
     3241 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaagttccg
     3301 gcagataaag ttcacgaact gcgtctgcgc ctggcacaac aaggtttgcc caaaggcggc
     3361 gcggtcggtt tcgaactact cgatcaggaa aagtttggta tcagccagtt cagcgaacag
     3421 gtgaattatc agcgggcgct ggaaggcgag ctttctcgta ccatcgcaac tatcggcccg
     3481 gtaaaagggg cgcgcgtaca tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa
     3541 aaatcccctt ctgcatcggt gacggtaaat ctgttacccg gccgcgcact cgatgaaggg
     3601 caaattagcg ccattgtgca tctggtttcc agcgccgttg ctggtctgcc gccgggaaac
     3661 gtcacgctgg tggatcaggg cggacatctg ttaacccagt ccaataccag cgggcgcgat
     3721 cttaatgatg ctcagttgaa atatgccagc gatgtcgaag gccgtattca gcggcgtatt
     3781 gaagcaatcc tgtcgcctat tgttggtaac ggtaatattc acgcccaggt cacggcgcag
     3841 ctggacttcg ccagtaaaga acaaacggaa gaacagtatc gccctaacgg tgatgaatct
     3901 catgcggcgc ttcgttcacg ccagcttaat gagagcgagc aaagcggttc cggttatccg
     3961 ggcggcgtac cgggggcgtt gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc
     4021 acgcctccgg caaatcaaaa taaccgccag cagcaggcga gcaccaccag caatagcggg
     4081 ccgcgtagca cacagcggaa tgaaaccagt aactacgaag tcgatcgcac cattcgtcat
     4141 accaaaatga acgtgggcga tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa
     4201 accttgccag acggtaaacc attgcctctc agcaacgaac agatgaagca aattgaagat
     4261 ctgacccgcg aggcgatggg cttttctgaa aaacgcggtg actcgctcaa tgtcgttaac
     4321 tcgccgttca atagcagtga cgaaagcggc ggagaactgc cgttctggca acagcaagcg
     4381 tttatcgatc agttgctggc tgccggtcgc tggttgctgg tactgctggt ggcgtggctg
     4441 ctgtggcgga aagcggtacg cccgcagtta acacgtcgcg ccgaggcgat gaaagctgta
     4501 cagcaacagg cgcaggcccg tgaggaagtg gaagatgcgg tggaagtccg cctgagcaaa
     4561 gacgaacaac tccaacaacg acgcgctaac caacgtctgg gggcagaagt catgagccag
     4621 cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg
     4681 ataaataacg atcatgagta acctgacagg caccgataaa agcgtcatcc tgctgatgac
     4741 cattggcgaa gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg aagtgcaaac
     4801 cctgagcgct gcaatggcga acgtcacgca gatctccaac aagcagctaa ccgatgtgct
     4861 ggcggagttt gagcaagaag ctgaacagtt tgccgcactg aatatcaacg ccaacgatta
     4921 tctgcgttcg gtattggtca aagctctggg tgaggaacgt gccgccagcc tgctggaaga
     4981 tattctcgaa actcgcgata ccgccagcgg tattgaaacg ctcaacttta tggagccgca
     5041 gagtgccgcc gatctgattc gcgatgagca tccgcaaatt atcgccacca ttctggtgca
     5101 tctgaagcgc gcgcaggccg ccgatattct ggcgttgttc gatgaacgtc tgcgccacga
     5161 cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg cggaactgac
     5221 cgaagtactg aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt
     5281 gagaacggca gccgaaatta tcaacctgat gaaaactcag caggaagaag ccgttattac
     5341 cgccgtgcgc gaattcgacg gcgagctggc gcaaaaaatt atcgacgaga tgttcctgtt
     5401 cgaaaatctg gtggatgtcg acgatcgcag cattcagcgt ctgttgcagg aagtggattc
     5461 cgaatcgctg ttgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga aattcctgcg
     5521 caatatgtcg cagcgtgccg ccgatattct gcgcgacgat ctcgccaacc gtggtccggt
     5581 gcgtctgtcg caggtggaaa acgaacagaa agcgattctg ctgattgtgc gccgccttgc
     5641 cgaaactggc gagatggtaa ttggcagcgg cgaggatacc tatgtctgat aatctgccgt
     5701 ggaaaacctg gacgccggac gatctcgcgc caccacaggc agagtttgtg cccatggtcg
     5761 agccggaaga aaccatcatt gaagaggccg aacccagcct tgagcagcaa ctggcgcaac
     5821 tgcaaatgca ggcccatgag caaggttatc aggcggggat tgccgaaggt cgccagcaag
     5881 gtcatgagca gggctatcag gaaggactgg cccgggggct ggagcaaggt ctggcagagg
     5941 cgaagtctca acaagcgcca attcatgccc ggatgcagca actggtcagc gaatttcaaa
     6001 ctacccttga tgcgcttgat agtgtgattg cttcgcgcct gatgcagatg gcgctggagg
     6061 cggcacgtca ggtgatcggt cagacgccaa cagtggataa ctcggcactg atcaaacaga
     6121 tccaacagtt gttgcagcaa gaaccgttat tcagcggtaa accacagctg cgcgtgcacc
     6181 cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac cttaagtttg catggctggc
     6241 gcttgcgggg cgatcccaca ctccatcctg gcggctgtaa agtctccgcc gatgaaggcg
     6301 atctcgacgc cagtgtcgcc actcgctggc aagaactctg ccgtctggca gcaccaggag
     6361 tggtgtaatg accacgcgcc tgactcgctg gctaaccacg ctggataact ttgaagccaa
     6421 aatggcgcag ttgcctgcgg tacgtcgcta tgggcgatta acccgcgcta ccgggctggt
     6481 gctggaagcc accggattac aattgccgct cggcgcaacc tgtgtcattg aacgccagaa
     6541 cggcactgaa acgcacgaag tagaaagcga agtcgttggc tttaacggtc aacggctgtt
     6601 tttaatgccg ctggaggaag tcgaaggtgt cctgcccggc gcgcgggttt atgccaaaaa
     6661 catttcggct gaaggactgc aaagcggcaa gcagttgccg ctcggtccgg cgttattagg
     6721 tcgcgttctg gacggcagcg gtaagccgct cgatggcctg ccctcccccg atacgacgga
     6781 aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa cgtacaccga ttgaacatgt
     6841 gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc gttgggcgtg ggcagcgtat
     6901 ggggctgttt gccgggtccg gcgttggtaa aagtgtgctg ctggggatga tggcacgtta
     6961 cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa cgtgggcgcg aagtaaaaga
     7021 ttttattgag aatatcctcg gtgccgaagg gcgtgcacgc tccgtggtga ttgccgctcc
     7081 ggcggatgtc tctccgcttc tgcgaatgca aggtgccgcc tatgccacgc gcattgccga
     7141 agattttcgc gatcgtggtc agcatgtgtt gctgattatg gactccctca cccgctacgc
     7201 gatggcccag cgtgaaattg cgctggcgat tggcgaacca cccgccacca aaggttatcc
     7261 cccgtcggtg tttgccaaat taccggcact ggtcgagcgt gccggaaatg gcattagcgg
     7321 cggcggatcg attaccgcgt tttataccgt gctcaccgag ggcgatgacc agcaggaccc
     7381 cattgccgac tccgcgcggg caatcctcga cggtcacatt gtgctgtctc gccgactggc
     7441 ggaagccggg cactatccgg ctatcgatat tgaagcgtcg atcagccgcg caatgacggc
     7501 gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt
     7561 tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct
     7621 cgacaaagcc atcgccctgt ggccgcagct ggagggctat ttgcaacaag gcatttttga
     7681 acgcgcggac tgggaagcgt ctctccaggg gctggagcgt attttcccga cagtgtcata
     7741 acccaggaga taacggcaga tggcagaaca tggcgcgctg gcgaccctga aagatctggc
     7801 agaaaaagag gtagaggatg ccgcgcgcct gctgggtgaa atgcgtcgcg gatgtcagca
     7861 ggcggaagaa cagctcaaaa tgctgatcga ttatcagaat gaatatcgca ataacctcaa
     7921 tagcgatatg agtgccggga tgaccagcaa ccgctggatc aactatcagc agtttatcca
     7981 gacgttggaa aaagccatta ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt
     8041 tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact
     8101 gcaggaacgg caatccacgg cggcactgct tgcagaaaac cgcctcgatc agaaaaagat
     8161 ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa tgattcgctt agcgccctta
     8221 attaccgccg acgttgacac caccacattg cctggcggca aagccagcga tgctgcacaa
     8281 gattttctcg cgttgttgag cgaagcatta gcaggcgaga caactaccga caaagcggcc
     8341 ccccagttgc tggtggcaac agataagccc acgacaaaag gcgagccgct ggtcagcgag
     8401 attcttgccg atgcgcaaca agcggattta ctgatccctg tggatgaaac accgcctgtc
     8461 atcaacgacg aacaatccac atcaacacca ttaaccaccg ctcaaacgat gacgttggct
     8521 gcggtggctg gcaacaatac ggcaaaagac gaaaaagcgg atgatctgaa tgaagacgtc
     8581 accgcaagcc tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg
     8641 actgatgtgc cgtcaaccgt gttaccggca gagaaaccaa cgctcttcac aaaactgact
     8701 tctgcgcaac tcacaacagc acagcctgat gatgcccccg gcacgccagc tcagccatta
     8761 acaccgctgg tagcagaagc ccagagtaaa gcggaagtca tcagcacacc ttcaccggtg
     8821 accgctgccg ccagcccgct aattactcca caccagacac agccactgcc caccgtcgcc
     8881 gcgcctgttt tgagtgcacc gctgggttct cacgaatggc aacaatcatt aagccagcat
     8941 atttcgctgt tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca cccgcaggat
     9001 ttaggtgaag tgcaaatctc cctcaaagtg gatgataacc aggcgcaaat ccagatggtt
     9061 tcaccgcatc agcatgtacg cgccgcgctg gaagcagcgc ttccggtact gcgtacgcag
     9121 ctggccgaaa gtggcattca gttagggcaa agcaacatca gtggcgaaag ctttagtggt
     9181 cagcagcagg ccgcttccca acaacagcaa agccaacgca cagtaaacca tgaacctctg
     9241 gcgggggaag acgacgatac gcttccggtt cccgtctctt tacaagggcg tgtaacaggc
     9301 aacagcggcg ttgatatttt cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc
     9361 cccgctttgt tgcgctcaag acgcaggata attagccgat aagcagtagc gacacaggaa
     9421 gaccgcaaca catgactgat tacgcgataa gcaagaaaag caagcgatcg ctttggatcc
     9481 cgattctggt attcattacc ctcgcggcct gtgccagcgc aggttacagc tactggcatt
     9541 cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt cgtaccctcc ccggtcttct
     9601 acgcgctgga taccttcacg gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca
     9661 taaccctgcg cctgaaagat gaagctaccc gctcgcggct gagtgagtat ttgccggaag
     9721 tccgtagccg tttgctgtta ctgttttcgc gtcaggatgc tgccgtactg gcgacagaag
     9781 aaggcaagaa aaacctgatt gccgagatta aaaccacgct ttccaccccg cttgttgccg
     9841 ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc ttttattctg cgataacgac
     9901 atgggcgata gtattctttc tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa
     9961 gtcaaagacg aaccgacagc cagtgttagc ggcgaaagtg acattcgtcc gtacgatccg
    10021 aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc
    10081 tttgcccgcc attttcgtat ggggctgttc aacctgctgc gtcgtagccc ggatataacc
    10141 gtcggggcca tccgcattca gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc
    10201 aacctgaacc ttatccatct gaaaccgctg cgcggcacag ggctggtggt gttttcaccg
    10261 agtctggtgt ttatcgccgt ggataacctg tttggcggcg atggacgctt cccgaccaaa
    10321 gtggaaggtc gcgagtttac ccataccgaa cagcgcgtca tcaaccgcat gttaaagctg
    10381 gcgcttgaag gctatagcga cgcctggaag gcgattaatc cgctggaagt tgagtacgtg
    10441 cgttcggaaa tgcaggtgaa atttaccaat atcaccacct cgccgaacga cattgtggtt
    10501 aacacaccgt tccatgtgga gattggcaac ctgaccggcg aatttaatat ctgtctgcca
    10561 ttcagcatga tcgaaccgct acgggaattg ttggttaacc cgccgctgga aaactcgcgt
    10621 aatgaagatc agaactggcg cgataacctg gtgcgccagg tgcagcattc acagctggag
    10681 ctggtcgcca actttgccga tatctcgcta cgcctgtcgc agattttaaa actgaaaccc
    10741 ggcgacgtcc tgccgataga aaaacccgat cgcatcatcg cccatgttga cggcgtcccg
    10801 gtgctgacca gtcagtacgg cactctcaac ggtcagtatg cgttacggat agaacatttg
    10861 attaacccga ttttaaattc tctgaacgag gaacagccca aatgagtgac atgaataatc
    10921 cggccgatga caacaacggc gcaatggacg atctgtgggc tgaagcgttg agcgaacaaa
    10981 aatcaaccag cagcaaaagc gctgccgacg cggtgttcca gcaatttggc ggtggtgatg
    11041 tcagcggaac gttgcaggat atcgacctga taatggatat tccggtcaag ctgaccgtcg
    11101 agctgggccg tacgcggatg accatcaaag agctgttgcg tctgacgcaa gggtccgtcg
    11161 tggcgctgga cggtctggcg ggcgaaccac tggatattct gatcaacggt tatttaatcg
    11221 cccagggcga agtggtggtc gttgccgata aatatggcgt gcggatcacc gatatcatta
    11281 ctccgtctga gcgaatgcgc cgcctgagcc gttagtgatg aataaccacg ctactgtgca
    11341 atcttccgcg ccggtttctg ctgcgccact gctgcaggtg agcggcgcac tcatcgccat
    11401 tattgccctg atcctcgctg ctgcctggct ggtaaaacgg ctgggatttg ccccgaaacg
    11461 cactggcgtt aatggtctga aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt
    11521 tgtggtggtc gatgtggaag atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa
    11581 tctgctgcat aaacttcccc cttcagcacc aacggaagag atacctcaga ccgattttca
    11641 gtcggtcatg aaaaacttgc ttaagcgtag cgggagatcc tgatgcgtcg tttgttgtct
    11701 gtcgcccctg tccttctctg gctggttacg cccctcgcct tcgcgcaact gccaggtatc
    11761 accagccagc cgctgcctgg cggtggacaa agctggtcgc tcccggtgca gacgctggtg
    11821 ttcatcacct cgttgacgtt tattccggca attttactaa tgatgaccag tttcacccgc
    11881 atcattattg tttttggttt attgcgtaac gcgctgggaa cgccctccgc gccacctaac
    11941 caggtattgc tgggactggc gctgtttttg acctttttta ttatgtcgcc ggtgatcgat
    12001 aaaatttatg tcgatgcgta ccagccattc agcgaagaga aaatatcaat gcaggaggcg
    12061 ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc gtcagacccg tgaggcggat
    12121 ttagggttgt ttgccagact ggcgaatacc ggcccgttgc agggacctga agccgtgccg
    12181 atgcgcattt tgctcccggc ctacgtgacc agcgagttga aaaccgcatt tcagataggc
    12241 ttcacgattt tcatcccttt tttgattatc gacctggtga ttgccagcgt gttgatggca
    12301 ttggggatga tgatggtccc ccccgccacc attgctctgc cctttaaact gatgctgttt
    12361 gtactggtgg atggttggca attgctggtc agttcgctgg cgcagagctt ttacagctag
    12421 agaggcaaaa tgacacctga atcggtcatg atgatgggga ctgaagcgat gaaagtcgcg
    12481 ctggcactag ctgccccgct gttgctggtg gcgctggtca cgggccttat catcagtatt
    12541 ttgcaggccg ccacacagat caacgaaatg acgctgtcgt ttataccaaa aatcatcgcc
    12601 gtatttattg ccattattat tgccggaccg tggatgctca atctgttgct ggattacgtc
    12661 cgcaccttgt tcactaacct gccgtatatc atcgggtagc ccgtacaatg atgcaggtga
    12721 caagcgatca atggctttcc tggttaagcc tctacttctg gccgttactg cgcgtgctgg
    12781 cgctcatttc taccgcgcca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg
    12841 gtctggcaat gatgatcacg ttcgctattg ccccatcatt acctgccaac gatgttcctg
    12901 ttttttcgtt ctttgctctg tggctggccg tgcagcagat cctgatcggc gttgcgcttg
    12961 gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc
    13021 aaatggggct gtcatttgcg acgtttgtcg atccggccag ccatctcaat atgcccgttt
    13081 tagcgcgtat catggatatg ctggcgttac tgctgttcct gacatttaac ggtcatttat
    13141 ggttgatttc actgctggtc gatacctttc acaccctgcc aattggtggc gaaccgttga
    13201 acagcaatgc gtttctggca ctcaccaaag cggggagttt gattttcctt aacgggctga
    13261 tgctggcgtt accgctcatt actctgctgc tgaccctgaa tctggcatta ggtttactga
    13321 atcgtatggc cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg
    13381 gtatctcttt aatggcggca ttaatgccgt taattgcgcc tttttgcgaa catttattca
    13441 gtgaaatttt taatttgctg gctgatatta ttagtgaatt gccattaata taattccgta
    13501 acgtttatca tgttatccta aggattatcc gaaaaataat acctgcgaac atcttccagg
    13561 atactcctgt ggcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttac
    13621 tttaagacaa ttccaggcaa attatacaac actttacggg atagtaagtc cgcctgaaaa
    13681 atcgcgagag tggcgcatta ggtgacccat gttgttccgt ttagtcatga tgaaatattc
    13741 aggtaagggg aattatcgtt acgcattgag tgagggtatg ccatgtcaac gattattatg
    13801 gatttatgta gttacacccg actaggttta accgggtatc tgttgagtag aggggttaaa
    13861 aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg ccatagcttg tgattcacag
    13921 cgcccttcag tggtgtttat taatgaggac tgtttcatcc atgatgcttc taacagtcag
    13981 cgcatcaagc acatcattaa tcaacatccc aatacgttat ttatcgtttt tatggcaatt
    14041 gccaatgttc attttgatga atatctattg gtcagaaaaa atttattgat cagttctaaa
    14101 tcgattaaac cggaatctct cgacgatatc cttggcgata ttctgaaaaa agagacaacg
    14161 ataacctctt ttttaaatat gccgacgtta tcattgagcc gaaccgaatc gagtatgttg
    14221 cgaatgtgga tggcaggtca gggaaccatt caaatctctg accaaatgaa tatcaaagcc
    14281 aagaccgttt catcgcataa aggtaatatt aaacgtaaga tcaaaacgca taataaacag
    14341 gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt tgtcaacatg
    14401 cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaataagat tactctgctt
    14461 tctccacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg ccgagcgggt
    14521 agtcttccag cgaaaccagg tacattgtgc cttcactaaa ctcctcaact gccagtacca
    14581 cgccaggacg acgcggaccg ccatccgttt tgactgttac ccgatcattc accttcatcg
    14641 ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc agcgccaggc
    14701 gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc ggacttaacg
    14761 actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg caaaagagta
    14821 cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa
    14881 tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg ttagcgtcga
    14941 tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg atattcatga
    15001 cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaatcccga ccctgagggg
    15061 gtcgggatga aacttgctta agcaagaagc acttaaaaaa ttcgttacac caggaaatct
    15121 gatgtgttca tcaccttatc cgcaattttt ttcgctgaca agaaaatatt cgccatatga
    15181 atgattaacg cttcttatga atagaaatgt gacgcacgtc accccttggt ggctctggcg
    15241 tcagcgggat cgctcccgaa aaaatcatct ttgataattt gtgcggtttt gagaatcatc
    15301 ctggtaagag gagtactgcg cgactatgtt ttcaattcaa caaccactac tggtttttag
    15361 cgatcttgat ggcaccctgc tggacagtca tagttatgac tggcaaccgg cagccccctg
    15421 gctcacccgt ttacgcgaag caaatgttcc cgtcattctc tgtagcagta aaacatcagc
    15481 ggaaatgctg tacttgcaaa aaacgttggg gctacaaggt ttaccgctga ttgccgaaaa
    15541 tggcgcagtg atccagcttg ctgagcaatg gcaggagata gacggttttc cacgcatcat
    15601 ctcaggtatt agccatggcg aaatcagcca ggttttaaat acgctacgcg agaaagaaca
    15661 ttttaaattc acgacttttg atgatgtcga cgatgcaacc atcgccgaat ggacgggatt
    15721 aagccgtagc caggcggcgc tgacgcagct acatgaggcg tcggtaacgc taatctggcg
    15781 cgacagtgac gagcgtatgg cacaatttac cgctcgtctg aacgaactgg gcttgcagtt
    15841 tatgcagggt gcgcgcttct ggcacgttct ggatgcctct gccggaaaag atcaggctgc
    15901 caactggatt atcgcgacct atcaacaatt gtcaggcaaa cgcccaacca cacttggcct
    15961 gggcgatggg ccaaacgatg cgcccttact ggaggtaatg gattacgcgg tgattatgaa
    16021 agggctaaac cgtgaagggg tgcatctgca tgatgaggat ccggcccgcg tctggcgaac
    16081 gcagcgtgaa ggaccggaag gctggcgtga agggctggac cattttttct ccgcccgtta
    16141 agcgttatcg ctcgcgaata cccgattgcg cccggcctgt ttagcgagat aaagtcgacg
    16201 gtcggccagt gactggagtt gttcaaaatc ataatcaccg gtttcctcgc tgctgcttac
    16261 ccccagcgag gcactgatgc gtatcgtcgt actcttagca atcaacatct ctttttcatt
    16321 tagcttcagg cgaatacgtt ctgcaacctc cgcagcctgc gtcagactcg cgcctggcag
    16381 aatcacacaa aactcctcgc caccgacccg cccggcaacg tcctgcgcac gcaaggaact
    16441 gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatggc caaagcggtc
    16501 attaatcgct ttaaaatggt caaggtcgac ctgaatgaca gaaaaaggat gttggtgcgt
    16561 ctgacacaat ttagcgagcg aacgggcttt ttcgaacagt gcgccactgt tatataaacg
    16621 cgttaaggtg tcgtgccacg cctgccactg caacaagctt tgcaaaacat acatgttgct
    16681 gaccatccgg cgaatcacat accaggagat gagtaacatg gtggtaaaga gcgcccacag
    16741 cagagttaat gcaatgctga tactgccgaa atcaccacgc acgccttcgc ttagcgtatg
    16801 gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc cagctaacat agcgactgtt
    16861 catacgaatg ccgccccgcg tgtcatgttc cattgcctgc gccagcaagg ccagttcacg
    16921 agggtcaaaa atattccctg ttggatggtc aggattggaa gaggtcaata atctcagctt
    16981 actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttc ttaaaaattg
    17041 ctgcatggta cgcacgggaa tactcatccc cagcacgcca taccagtagt tttcactgtc
    17101 taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg gttgtgaagt
    17161 gaaccagcgt accgcgcggt gacgattttc tcgttgcgaa tggccgacaa accagggttg
    17221 ggtgacatag ccgtaataac gcgttggtac attgcgcgta aataaggtcg gctgcgtcga
    17281 aacgtaaaat ccggcacgcg agacatacat cgcctgttca accatcgacg aggagttgtg
    17341 cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt cgaggctttc
    17401 attttcgcga gacaggagat tcccctcgct gactaatgca tccgacacac cattgactgg
    17461 cagggtgcgt cgtcggttga gttcgatttg ccaggcgtgc tcgtcgcgat gctgttcaaa
    17521 ctcggtaacg gcattacgca gagaggtgaa atccagtggc gctacgagag cttcgcgcat
    17581 gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat cgagcgcgtt
    17641 ggctacgttt tccagatgat tacgctggct ggagatatag gcatcttcca gcaccaccac
    17701 ttcacgccag gtgagcaagg tggaaaaaag caataccacg ataaagcaga gattaacgac
    17761 atgaccaggc cccaggcggc gtgcgagttt tttcaaccag ctctggtttt ccatttttgt
    17821 ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca taacccggcg
    17881 ataacgtacc gcaaaacagc gataatttta taatggtggc atgataagac gcgactatca
    17941 ttcattgcag gcattggcac accaggccgg atgtgaaccg gcctggtgtt aatgataccc
    18001 tttcaaactt cttccgcaga acgactcccc tcccccggaa caagctcggt ttcatgaaaa
    18061 gcttcccgct tgaccccata accgtcatac caacgacact caaccatacc gctggagtat
    18121 ccggtgacaa tcatccgcgg gccgccctct ttaaccgtaa cttcctcact aaccataaag
    18181 ctcatactcg cctccttttt tcgagtgaaa cttgttcacc ttagttgaag atggcgaatt
    18241 ttgcataata gcctgtgcga gtattaatgt gcctgaccgc gcattttcgc tactgctttc
    18301 actcccagca ccacgatgcc gccgatgata aacccaagaa tcagatttaa aacagtcggt
    18361 aatatcatcg ccaccactgc actttgctgc ccggcgaaat gttcaatggc gtgatgcagc
    18421 ggcgcaatac catgtaccac aatcccgccg ccgacgagga acatcgccag cgtgccgaca
    18481 atcgataacg ctttcatcaa ccagggcgcg ataatcaata accctttacc taatgcctgc
    18541 atcagcgcgc tggatttttc cgccagccaa taccccaggt catcaatctt aacgataacc
    18601 cctaccagac catagacgcc cacggtcact accagcgcga tgcctgaaag caccagcacc
    18661 tgattaagca acggcgcttc tgccacaatc cccagcgtga tggcgacgat ttccgcagac
    18721 aatataaaat cggtacgaat cgcccctttt attttgtcct tttcaaactt cagcggatcc
    18781 tgcgccgcca gcttctccag acgctgctga ctctgcgccg gatcttcttt atgtttacgc
    18841 gcctccagca tatgcagcac tttctctacc ccttcaaagc agagaaacgc gccgccaatc
    18901 atcaacagtg gcgtaatcgc ccacgggata aacgcactga tgatcagcgc cagcggcacc
    18961 agaatcactt tattgatcag cgatcctttc gccacgcccc agaccacggg aagttcccgg
    19021 ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata agtcatcccc taatacaccg
    19081 gcggttttct tcgccgccag tttgcccatc acggaaatat cgtccagcag tgtggcgata
    19141 tcatcgagca acgtcagtaa actactcccg gccaaaagca ttccctctca tttttattgt
    19201 tgaagggtgt aagtatgaag caaaaacggc agctctgata cttgcttcac aggtcaacat
    19261 ttttttaaca atcaagtgca attatatctc tcgccagcag aatgattttc acgtttacta
    19321 tgacccacct ttttatttcc accgtgaggg attatgcgtt tccgccagtt gttaccgctt
    19381 tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa cctattttgt cattcggatt
    19441 ggcgtggaaa gctggccgcc gttaatgatg gcaggcgtgc gattcctggc agccggtatt
    19501 ttattgctgg catttttgct actgcgcgga cacaaactcc ccccgctacg tccgctgctc
    19561 aatgccgcgc tgattggcct gttattgctg gctgtcggta atggcatggt aacggttgcc
    19621 gaacatcaaa atgttccttc cggcatcgcc gccgtagtgg ttgcaaccgt gcccctcttt
    19681 accctgtgct tcagccgcct gtttggcatt aaaacgcgca aactggaatg ggtgggtatt
    19741 gccattgggc ttgccggaat catcatgctc aatagcggtg gaaatttaag cggcaatccg
    19801 tggggcgcga ttctgatttt aatcggctcg attagctggg cgtttggctc ggtttatggc
    19861 tcgcgcatta ccttacctgt agggatgatg gcgggtgcga ttgagatgct ggcggcaggc
    19921 gtggtgttaa tgatcgcgtc gatgattgca ggtgaaaaac tgacggcact cccttccctt
    19981 tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt cgattatcgc cattaacgct
    20041 tatatgtatt taatccgcaa cgtcagtccg gctctcgcca ccagctacgc ctacgttaac
    20101 ccggtggtcg cggtcttgct gggtacggga ctgggcggag aaacgctgtc gaagattgaa
    20161 tggctggcgc tcggcgtaat tgtcttcgcg gtggtactgg tcacgttggg aaaatatctc
    20221 ttcccggcaa aacccgtagt tgcgccagtt attcaggacg catcaagcga gtaaatgaat
    20281 cccctgcgtg tcgatctgcg cactggcacc ttcgccgcag atccactctt ccagacgctc
    20341 gctaagcgct tcatccgtca gcttctcgcg cccacgtaat gcacactccc agacaatcaa
    20401 tacgcgacag ccgagttcct gcaaacgact gatatcgcgg cgatcgcgtt caacattttt
    20461 acctatcttc tccagccaga actcggttcg agtcgcaggc actttaaaca gatagcagtg
    20521 atgatgatgc cagaagcagc cgtgggtaaa tatcacgcag cgatattcat caacgacaaa
    20581 atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac gccaggccca gcccggttaa
    20641 cagactggcg aggcgcttct ctatcgccgt atcacgcgtg gcaatcgcgc gcatattttt
    20701 gctgcgagtg gccttatcgt gaacgtcggc catgttgtgc ctcttgctga cgcaacgcca
    20761 ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc ggcaaagacc ggcacgacca
    20821 ccgaattacc gaactggcga taggcctgag tgtccgaaac cggaatgcgg aatttcgctt
    20881 ctcccggcgc ttcaaaaccc attaagcgcg cgcattcccg aggcgttaac cgacgtggac
    20941 gatgttgctg attcagcgga tcgtcaaaat ctttctcacc cgtggccata tcccagccgc
    21001 gatcgatcaa aatttccgcg ccatctttgt aataacgcgc agacaacgta cgcgtgacgc
    21061 tttgcggatt gttcggataa accattccat aaccgaagcc attaccgcgt gcctgatgtt
    21121 ttttcgcata acgatagagg tacttccaca gcaccggcgt caggatatat ttcgcctcga
    21181 ccatcgggtc cagcagctgg gccagcgtca ctcgctgcgc agggaaacat tcgctgatat
    21241 cacgcagagt aaaatcggct ttcagattca gatcgcgacg aaaacccacc agcacgatgc
    21301 gttcacggtg ctgcggcagg aaatgtttgc catcgatgat tttcggatcg tccggcccgt
    21361 tatcttcagc gtcggcaacg tcatagccca gttcgtcaag cgtctgcatg atgatgcgga
    21421 acgttttacc ctggtcgtgg cttttcaggt ttttgacatt ttcgagcaca aaaatcgccg
    21481 gtcgacgcgc gtcgataata cgcaccacat caaagaacag cgtgccctgg gtatcacagg
    21541 caaaaccgtg cgcccgcccg agcgagttct ttttcgatac gccagccagc gaaaatggct
    21601 gacaaggaaa accggccagt aatacatcgt gttcaggaat gtgttgacga atatgttccg
    21661 ctgccgcctc atcactcacg ccgtctttat ggctgagggt gatgtcgcgg atatcttcat
    21721 taaaatgatg cgtcgccgga tcgcaataat ggttggcttt ataagtgcga accgcatgtt
    21781 tgttccattc gctggtaaag acgcactgtc cgccaatcga ttcaaaaccg cgacggatgc
    21841 cgccaattcc ggcaaataga tcgataaagc gaaacgcata atgcggatga tgtgccggag
    21901 gtttgggtaa caacgtttgc agatgggcga actccttctc acttaaacgg tgccatgccg
    21961 agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca gtgattctca cccacaccat
    22021 taagctgcgc caccagcgtt ttaacatcat aaatttgcag cagtttctcc agcattgcct
    22081 gtgcggcatt cccggtgctg tatgaatcgg ttactgatat attttcctgc atagatttaa
    22141 ccggccatct gagatggccg acaggttaac ataattacag ccatttaggg ccacgcgctc
    22201 agcccgcggg tgaaaacgca tctatcacct tgtgatcgac accttcattc tcccccgcca
    22261 gttcggcact gagcttcgcc ataaactcga ctaaaaattg cgcgttgtgc tgcgccagct
    22321 gcttgcctct tgcggtttgc atggtttgcg gtagcttcag caacttagtc tggaaatgat
    22381 ccagcgcgta gcgtttatca tcaagcgggc gatgctgtgc gaacggatct tcaccatcaa
    22441 acagcgccac gcccaatgcc cctgaaacgg caaacacgcg cgccaggcca atcgccccca
    22501 gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag ggggcgattt
    22561 gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcgattttc tccgccggaa
    22621 attgctcgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg attgaagaac
    22681 gctgccgttg cggatgattt ttcgccaggc tcacaatatc gtggaaataa caggcggtta
    22741 aaatcaccag catatcgacg tcatcatctg ccgccagctt ctgtgccgta gcccagacgc
    22801 ggcgaaaatg atacacgtca tgggccgcgt cctggtgctg atgatggttt ttcaaccagt
    22861 tttcgaactg tgtctgccag tgttgtaagt ccatacgcgc tccgttgcca aagagtcgcc
    22921 accttagcag tttttattca tagcgtctgc tacgtgcggg acgaggtcgg gtgtaccagg
    22981 caatgctgcc taacaatgcc ccgaccagag ccataaataa aaatccgacc agtgccccta
    23041 accactttcc cgcggtagag cctaaatcgc tttcaaatcc cgacacgggg gcatccggct
    23101 cacccccaga tgtaacgcgc cattgcccag acgaaatagc cacaccatgt atagaaagcc
    23161 cacaaagcca gtttgccacc agggctgcga gagagcctct cttccatctt tgtcgaaccg
    23221 tattccattt tgagataacc cggaaaattc agacttagta ctattatgtg ataaaagtca
    23281 catttttcca cattgaatga gccagtcaag actcaatcat tttttgaaca aatagataaa
    23341 ttttgcgcaa cggcagtgaa tacaggaaaa atcgttttga gcgattttat ttatctttga
    23401 tatcg
//
LOCUS       DAPHDX010000071        23332 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPHDX010000071 DAPHDX010000000
VERSION     DAPHDX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.071"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            108..650
                     /locus_tag="RH058_002701"
     CDS             108..650
                     /locus_tag="RH058_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3138272.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            733..1434
                     /locus_tag="RH058_002702"
     CDS             733..1434
                     /locus_tag="RH058_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3138273.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1459..4058
                     /locus_tag="RH058_002703"
                     /pseudo
     CDS             1459..4058
                     /locus_tag="RH058_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4049..5119
                     /locus_tag="RH058_002704"
     CDS             4049..5119
                     /locus_tag="RH058_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3138274.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5131..5673
                     /locus_tag="RH058_002705"
     CDS             5131..5673
                     /locus_tag="RH058_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3138275.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5681..6196
                     /locus_tag="RH058_002706"
     CDS             5681..6196
                     /locus_tag="RH058_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3138276.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6162..6899
                     /locus_tag="RH058_002707"
     CDS             6162..6899
                     /locus_tag="RH058_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3138277.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7010..8020
                     /gene="pyrD"
                     /locus_tag="RH058_002708"
     CDS             7010..8020
                     /gene="pyrD"
                     /locus_tag="RH058_002708"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV3138278.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8194..8736
                     /gene="zapC"
                     /locus_tag="RH058_002709"
     CDS             8194..8736
                     /gene="zapC"
                     /locus_tag="RH058_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV3138279.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8733..9842)
                     /gene="ycbX"
                     /locus_tag="RH058_002710"
     CDS             complement(8733..9842)
                     /gene="ycbX"
                     /locus_tag="RH058_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV3138280.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10086..12194
                     /gene="rlmKL"
                     /locus_tag="RH058_002711"
     CDS             10086..12194
                     /gene="rlmKL"
                     /locus_tag="RH058_002711"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV3138281.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNHQIHNCWLITAA"
     gene            12206..14113
                     /locus_tag="RH058_002712"
     CDS             12206..14113
                     /locus_tag="RH058_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3138282.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14243..15496
                     /gene="pqiA"
                     /locus_tag="RH058_002713"
     CDS             14243..15496
                     /gene="pqiA"
                     /locus_tag="RH058_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV3138283.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15501..17141
                     /gene="pqiB"
                     /locus_tag="RH058_002714"
     CDS             15501..17141
                     /gene="pqiB"
                     /locus_tag="RH058_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV3138284.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17138..17701
                     /gene="pqiC"
                     /locus_tag="RH058_002715"
     CDS             17138..17701
                     /gene="pqiC"
                     /locus_tag="RH058_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV3138285.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17957..18124
                     /gene="rmf"
                     /locus_tag="RH058_002716"
     CDS             17957..18124
                     /gene="rmf"
                     /locus_tag="RH058_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV3138286.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18194..18712)
                     /gene="fabA"
                     /locus_tag="RH058_002717"
     CDS             complement(18194..18712)
                     /gene="fabA"
                     /locus_tag="RH058_002717"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV3138287.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18781..20541)
                     /locus_tag="RH058_002718"
     CDS             complement(18781..20541)
                     /locus_tag="RH058_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV3138288.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20727..21179
                     /gene="matP"
                     /locus_tag="RH058_002719"
     CDS             20727..21179
                     /gene="matP"
                     /locus_tag="RH058_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV3138289.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21255..22295)
                     /gene="ompA"
                     /locus_tag="RH058_002720"
     CDS             complement(21255..22295)
                     /gene="ompA"
                     /locus_tag="RH058_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV3138290.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22652..23161)
                     /gene="sulA"
                     /locus_tag="RH058_002721"
     CDS             complement(22652..23161)
                     /gene="sulA"
                     /locus_tag="RH058_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV3138291.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
BASE COUNT         5978 a         5471 c         6096 g         5787 t
ORIGIN      
        1 attggattta gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg
       61 tcatttaaag gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg
      121 tattgacggc gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata
      181 atgctatcac ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc
      241 tggtagctgc gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc
      301 aaactaatgg agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg
      361 atgttactgt aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac
      421 ctacagcatt tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt
      481 atctgccaga tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag
      541 cggattctgc tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga
      601 cttctggtaa tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat
      661 taattgtttt attaattagt acccctctag tgttctggag gggatattca tattttttaa
      721 gagtgactat ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca
      781 atattactca gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca
      841 acacgtcttg tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt
      901 ccggatgttc cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca
      961 gattttatta ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc
     1021 attatgtaca ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta
     1081 cgggcagtcc cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa
     1141 agcgtcatca aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg
     1201 gcgaaactgc gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat
     1261 ttcattaccc tgacagacct gaaaataggt ggaaaagtag ttaaaaatca aatgatttcc
     1321 ccctttgata aataccaatt ttctctgcca aagggggcca aaaatagcag cgtaacgtat
     1381 cgaaccatca atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc
     1441 ttcagttaag agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt
     1501 ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa
     1561 gctgaaacct ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg
     1621 tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg
     1681 aatcagacaa ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg
     1741 attgccgcct gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt
     1801 ccggctttta aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat
     1861 gccagcgtaa cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc
     1921 atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg
     1981 ctattactgg gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat
     2041 tctggcgaca gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg
     2101 cgcaacaatt caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc
     2161 agctatttgc agcgggcggt tattccacta aaaggcgaac tgaccgttgg tgatgattat
     2221 actgcaggcg attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac
     2281 aacatgctgc cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc
     2341 aatgcccaga taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct
     2401 ggtgcttttg aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa
     2461 atcaaagaag cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta
     2521 ctccagcgtc aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt
     2581 aatgaacagc aggagagcaa atttgcccag gccacgttgc agtggggcgg accgtgggga
     2641 acgacatggt atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg
     2701 ggatttaacc ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg
     2761 ctggcagacc aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc
     2821 aaccaattgg gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac
     2881 accctttccg acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat
     2941 gaagatacgc cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg
     3001 caggtcaata tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag
     3061 caaacttact ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa
     3121 attaaagatc tctcgctggg ggtttcctgg aactacagta agtcccgtgg tcaacctgat
     3181 gctgatcagg tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt
     3241 aatgatagct ataccaggaa aaaaaattac gcctggatga cctctaacac cagtatcgat
     3301 aacgaagggc acattacaca aaacctgggt ttaacggaga cactactcga tgacggtaac
     3361 ctgagctaca gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc
     3421 agcatggact acaaaggggc gtttgcagat gcccgagtgg gctacaacta cagcgataac
     3481 ggcagtcaac aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc
     3541 attaccctgg ggcaatcgct gggggaaact aacgtgctga ttgcagcacc aggcgcagag
     3601 aatactcgtg tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtc
     3661 cctatgccac ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta
     3721 acgtggatct tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg
     3781 cggagttcaa tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac
     3841 cgctgcgttt tggcgcgata gcgacgctgg acggcataca gactaatagc ggcattattg
     3901 atgatgatgg ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct
     3961 ggggcgaagc tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta
     4021 actctgcgat tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt
     4081 attactacga ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta
     4141 tagcgggcag tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa
     4201 aaccctggag gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa
     4261 cgaatcgaat gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg
     4321 atgggcattc gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa
     4381 tgactatctt gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc
     4441 tttcgaaaac gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca
     4501 acgtttaggt ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca
     4561 gccgttcgtt ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta
     4621 caacataccc gcaggtgatt cctgcacgac tacaggtaca tcggttttag tgtattacct
     4681 gtctggtacg atcaattcac ttggctcatg ttccgtcaat gccggagaga ctatcgaggt
     4741 cgatctgggc gacgtatttg cggctaactt tcgtgttgta gggcataaac cccttggggc
     4801 cagaacggca gaactcgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa
     4861 cgtcaacctg agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc
     4921 acgtcctggc gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg
     4981 tggaacatta ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta
     5041 tccagtcagc acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt
     5101 gagaataaat tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt
     5161 atcttgttct ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt
     5221 atgcatattt atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta
     5281 caaaatattc atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc
     5341 actgctgctg atttgaatat cgatatcacc ggttgtgccg ctggtattac tggcgcggac
     5401 gtccttttta gcggcgaggc tgacaccctt gcgccgacac tgctcaaact aactgacaca
     5461 ggcggaagcg gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag
     5521 caagaaatcc cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca
     5581 ctcaaatatc aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg
     5641 gcggttctct attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg
     5701 gattctgttc ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacagt
     5761 caacattagc ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat
     5821 caatgtgcag ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga
     5881 gaaatctttc atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac
     5941 cttttcagga acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga
     6001 tgcagccagc gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa
     6061 ccaggcgagt aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca
     6121 attgcgacct gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt
     6181 acttcattat gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc
     6241 tgctggtggc gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc
     6301 cggcagacag agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta
     6361 ttaacagtaa aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag
     6421 caccattact ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc
     6481 gcttgctgag aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa
     6541 gcattgccag cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct
     6601 cggtatttaa actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc
     6661 aacaattacg ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact
     6721 acattaacct gattcagttg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc
     6781 cgcctaaaag ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct
     6841 ggcgggcgat aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa
     6901 acaggttcgg aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac
     6961 tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc
     7021 cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca
     7081 gcaattacgc cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc
     7141 gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct
     7201 tgataaagac ggggagtgca ttgacgcgtt aggcgcgatg ggatttggct cgatcgagat
     7261 cggtaccgtc acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt
     7321 agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt
     7381 agagaacgta aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa
     7441 agatacgcca gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc
     7501 ctatgcggga tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct
     7561 gcaatatggt gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt
     7621 gcaagcgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga
     7681 agaagaattg atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc
     7741 aaccaatacc acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg
     7801 tggcttaagt ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact
     7861 ggaattaaac ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc
     7921 gcgtgaaaag attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa
     7981 aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct
     8041 ttataagata atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct
     8101 tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg
     8161 ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg
     8221 cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta
     8281 tttcgctcac gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt
     8341 tgcgttgacg acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta
     8401 tctaaagaac aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa
     8461 ccgcaaatgc cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg
     8521 ggcgatgcgg cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt
     8581 gaatctggcg aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt
     8641 cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt
     8701 aatgttgaca gcttcagcct cgaacaggct gtctaacgcg ccaacttaag tgcagtcttc
     8761 ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc
     8821 gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa
     8881 taagggatac gaattccctg attttctaat tgctccagca acacctgttg gttatttcca
     8941 cgaaatgcct gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg
     9001 gcggtatcat cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc
     9061 acctcatcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg
     9121 acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc
     9181 ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta
     9241 accacatcaa acaccacatc accaatgcga atcactttcc agctatcttc ttcccaggct
     9301 gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa
     9361 cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca
     9421 aatgacaggg gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag
     9481 cgtaattgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca
     9541 attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca
     9601 gcaaaacgaa catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca
     9661 ggtgaaggag taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca
     9721 ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt
     9781 gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc
     9841 acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc
     9901 tataatgcgc aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg
     9961 gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt
    10021 ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg
    10081 acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg
    10141 aactggaaaa cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg
    10201 gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt
    10261 tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga
    10321 tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga
    10381 atgacaccat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg
    10441 ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg
    10501 ttaacgtctg gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt
    10561 tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag
    10621 ccgctattgt gatgcgatcc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg
    10681 gttccggtac gttgctgatt gaagcggcga tgctggcgac cgatcgcgca ccaggcttgc
    10741 accgtgggcg ttggggcttt agcggctggg cgcagcatga tgaagctatc tggcaggaag
    10801 tgaaagcgga agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt
    10861 acggttcgga cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg
    10921 ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc
    10981 cgaaagggcc gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg
    11041 aaccggcgct gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg
    11101 gctggaatct ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg
    11161 cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg
    11221 ttgccgaaag cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc
    11281 tgcgtaagaa cctcaaaaaa ttcgagaagt gggctcgtca ggaaggaatt gaatgttacc
    11341 gcctgtatga cgccgatctg ccggaatata acgttgccgt tgaccgttat gccgactggg
    11401 tggtggtgca ggagtatgca ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc
    11461 tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc
    11521 tgaaaacccg tgaacgtcag aagggcaaaa atcagtacca gaaactgggc gagaagggcg
    11581 agtttctgga agttaccgaa tataacgctc acttgtgggt gaatctgacg gattacctcg
    11641 ataccggtct gttcctcgat caccgtatcg cccgtcgtat gctcggccag atgagcaaag
    11701 gcaaagattt cctcaacctg ttctcttata ccggcagcgc caccgtgcat gcgggattag
    11761 gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac
    11821 gcaacctgcg tctgaatggc ctaaccgggc gtgcgcatcg cctgattcag gccgattgcc
    11881 tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct
    11941 tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga
    12001 tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac
    12061 gtggcttccg tatggatctc gacgggctgg caaaactggg actgaaagca caagaaatta
    12121 cgcaaaaaac gctctcccag gatttcgccc gtaaccacca gatccacaac tgctggctga
    12181 ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggtg catggctgtc
    12241 gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt
    12301 ttgtctggta ggccgtaacg gcgcgggcaa atcgacgtta atgaagatcc tcaaccgtga
    12361 acaggggctg gatgacgggc gcattattta cgagcaagat ttgatcgtgg ctcgtctgca
    12421 acaggatccg ccgcgtaacg ttgagggcag cgtttatgat ttcgttgccg aaggcattga
    12481 agaacaagcg gaatacctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc
    12541 gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct
    12601 gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt
    12661 tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt
    12721 gagcaatccg cgcgttttgc tgctcgacga accgactaac cacctggata tcgaaaccat
    12781 cgactggctg gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga
    12841 ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt
    12901 gacctatccg gggaattacg accagtacct gcaggaaaaa gaagaagccc tgcgcgtgga
    12961 agaactacaa aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca
    13021 ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg
    13081 tcgcgagcgt ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc
    13141 cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa
    13201 gcaactggtg aaagattttt ctgcccaggt tctacgtggc gacaaaattg ccctgattgg
    13261 tccgaatggg tgcggcaaaa ccacgctgct aaaactgatg ctcggtcagc ttcaagcgga
    13321 cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc
    13381 ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat
    13441 ggttaacggc aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa
    13501 acgggcgatg acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc
    13561 gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga
    13621 tgtcgaaacg ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct
    13681 ggttagccac gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg
    13741 cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca
    13801 gtatgtggcg ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc
    13861 agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca
    13921 gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaagtggc
    13981 ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa aaagtgcttg ctgatatggc
    14041 tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa
    14101 aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt
    14161 gcctgatgaa ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat
    14221 agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg
    14281 ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg
    14341 tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc
    14401 ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat
    14461 gaacgttgca ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc
    14521 tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt
    14581 ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga
    14641 gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc
    14701 tggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag
    14761 ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg
    14821 tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg
    14881 cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc
    14941 tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct
    15001 gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt
    15061 gcccatcatg gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt
    15121 cattctgtta tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat
    15181 tatggtgcca acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca
    15241 tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg
    15301 ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg
    15361 tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac
    15421 aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga
    15481 gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga
    15541 agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc
    15601 ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag
    15661 gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa
    15721 gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta
    15781 tggaaaaatt gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg
    15841 aagggattag cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg
    15901 cgaaaggcag caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg
    15961 atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag
    16021 atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacac
    16081 aaaaacgcaa tatcagctat caactgttca tcaatgcacc ttatgaccga ctggtgacca
    16141 acaatgttcg cttctggaaa gatagtggca ttgcggttga tctgacgtca gcagggatgc
    16201 gtgtggagat gggctcattg acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg
    16261 aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc
    16321 agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag
    16381 attcggtacg cggtctgcaa ccgggagctc cggtagagtt ccggggtatt cgcctgggta
    16441 ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc
    16501 gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg
    16561 cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa
    16621 ccggaaacct ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg
    16681 caataaccgg tattcgtgaa tttaatggtt atcagattat cccgaccgtt agcggcggcc
    16741 tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga
    16801 atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc
    16861 tgcaaacgac gctggatagc atgaacaaga tcctcgctag ccagtcgatg cagcagttgc
    16921 cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg
    16981 gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag
    17041 aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga
    17101 aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat
    17161 tgcagcactg tggctggtcg gatgcagctc cggcgaaatt aataaaaact attaccagtt
    17221 acctgtggtg cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt
    17281 agagcaggtc actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga
    17341 tgtgaagtat gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg
    17401 caacaccctg gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc
    17461 tctgggaagc gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga
    17521 tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg
    17581 tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct
    17641 ggccggtgtc tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta
    17701 attataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtc
    17761 tctaacgtgc ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt
    17821 agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt
    17881 attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt
    17941 atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg
    18001 tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa
    18061 tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc
    18121 ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc
    18181 tgtctgatta taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg
    18241 cggtatagat cagacgacca tcaaccagca cttcgccatc cgccaggccc ataatcagac
    18301 gacggttaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta
    18361 cctgaccagt gaatttcact tcgccaacgc ccaacgcgcg gcctttacct tcgccgccca
    18421 gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca
    18481 taaccggatc gccaataaag tggcatccga agaaccacag atccggattg atatccagtt
    18541 ctgcttcaac ataccctttg tcgaagtttc cacccgtttc ggtcattttg accacacggt
    18601 ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc
    18661 gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg
    18721 taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga
    18781 tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc
    18841 ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc
    18901 gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca
    18961 gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg
    19021 aacgttagct gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat
    19081 agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg
    19141 atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat
    19201 cagggcgcag agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc
    19261 aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa
    19321 cgcttgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc
    19381 gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc
    19441 aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg
    19501 ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc
    19561 gaggaaacct tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa
    19621 ggtgtcgcca tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct
    19681 aagcggaagt gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca
    19741 ggcatccgct cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca
    19801 ccactgggtt actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat
    19861 agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc
    19921 taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga
    19981 ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca
    20041 cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg
    20101 atgcaccaga ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag
    20161 ttgctccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc
    20221 tgcactcact gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac
    20281 cagttggcct gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc
    20341 agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg
    20401 ctccagtgcg aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat
    20461 caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca
    20521 tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat
    20581 accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg catgggcgtt
    20641 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    20701 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    20761 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca
    20821 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg
    20881 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    20941 aaaccattcg ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    21001 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg
    21061 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    21121 acgcgaataa aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac
    21181 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    21241 taccagacga gaacttaagc ttgcggctga gttacaacgt ctttgatacc tttaacttcg
    21301 atctctacgc gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg
    21361 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    21421 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    21481 ccctggttgt aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg
    21541 tctttcggat ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    21601 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    21661 gtctgtactt ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga
    21721 ccgaaacggt aggaaacacc caggctcagc atgccgttgt ccggacgagt gccgatggtg
    21781 tgtgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    21841 atcgcgtact caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca
    21901 taaacgttgg atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc
    21961 aggtcgtcag tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat
    22021 gcaccgtttt caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca
    22081 aagccaacat acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca
    22141 tgggtcgggc cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta
    22201 gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc
    22261 agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa
    22321 atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct
    22381 caatattaac tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct
    22441 acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg
    22501 tataaggtat gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc
    22561 gcttcagaca agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca
    22621 aaaaaagttc caggattaat cctaaattta cttaatgata caaattagag tgaattttta
    22681 gcccggaaag ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata ataaacccca
    22741 tagcgttacc ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat
    22801 ctgccaacca accgatcacc acactgtaat tgcccgtgcg taaagcgcga accattgact
    22861 ccacagtgtg gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag
    22921 atgcctgaac ccattcccga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg
    22981 attgctgacc gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat
    23041 cttcgcgata gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg
    23101 caattttact tgctgcggat gagaacgacg aagaacgatg tgcatagcct gaagtgtaca
    23161 taatcaatcc agcccctgtg agttactgta tggatgtaca gtacatccag tgacaacaaa
    23221 gatcaaccct attttcggaa agagcctcgc aaattttgtc gttggtgacg ggaaaacata
    23281 aattaatctt gccccttaag aataagttgc ctattttcgt agttaacgga tc
//
LOCUS       DAPHDX010000072        22655 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPHDX010000072 DAPHDX010000000
VERSION     DAPHDX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.072"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..221
                     /locus_tag="RH058_002722"
     CDS             <1..221
                     /locus_tag="RH058_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDV3138292.1"
                     /translation="PRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLK
                     NDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            complement(443..672)
                     /locus_tag="RH058_002723"
                     /pseudo
     CDS             complement(443..672)
                     /locus_tag="RH058_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            851..1588
                     /locus_tag="RH058_002724"
     CDS             851..1588
                     /locus_tag="RH058_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3138293.1"
                     /translation="MKKVIAVSTLAMAGMFSAQTLADENKTGFYVTGKAGVSVMSLSE
                     QRFVDGEGAWADKYKGSDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESAGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGAGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
     gene            2012..2908
                     /locus_tag="RH058_002725"
     CDS             2012..2908
                     /locus_tag="RH058_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV3138294.1"
                     /translation="MVDSTRNRSAERLIDILVELQNYGVVSRYNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAICNSNVCQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDV
                     VLFIYSNIAHYFLRRDLLPYQQLLHKHDNGITLSCKAAHKNQIVPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            2957..4036
                     /locus_tag="RH058_002726"
     CDS             2957..4036
                     /locus_tag="RH058_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDV3138295.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDILEKAKERYE
                     EHKEKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIQNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACDVARAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKDELDDVLASSVVKFDDFSSQS"
     gene            4083..5654
                     /locus_tag="RH058_002727"
     CDS             4083..5654
                     /locus_tag="RH058_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HDV3138296.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGEYDSHHYHSHENYIATNREGKRAHQAGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAELNHFSLHLDSVNSEIERGLLLREEVRRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            5651..5917
                     /locus_tag="RH058_002728"
     CDS             5651..5917
                     /locus_tag="RH058_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3138297.1"
                     /translation="MKLHPPFPCNQCGACCRHVNRAGETQCLDRGDGICRHYQTDSHL
                     CAIYDKRPQICRVEDQYLLNYQSQYSWQEFIALNQAACLILNKL"
     gene            6057..6254
                     /locus_tag="RH058_002729"
     CDS             6057..6254
                     /locus_tag="RH058_002729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138298.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRYLLSRKKTS"
     gene            6320..7993
                     /locus_tag="RH058_002730"
     CDS             6320..7993
                     /locus_tag="RH058_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV3138299.1"
                     /translation="MYSSATVCFTVSAGQKYRFYSIIMKINLPFIHWSRISNDKRLVL
                     SVVVFTLSNILVSSGAHLIYRILFFTLALLIIKAMKSLTLRFILAFSFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPLEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARADNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSVNTVLHHDVRHYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGGRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPALGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNEPLE"
     gene            complement(8137..9180)
                     /locus_tag="RH058_002731"
     CDS             complement(8137..9180)
                     /locus_tag="RH058_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV3138300.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDIWFGPLLGEFEELLDAYDTDEISE
                     TSYINKLRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHTSIQHR"
     gene            complement(9614..10804)
                     /locus_tag="RH058_002732"
     CDS             complement(9614..10804)
                     /locus_tag="RH058_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3138301.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQTTDILLQLKTFSGQYQLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            complement(10981..11056)
                     /locus_tag="RH058_002733"
     tRNA            complement(10981..11056)
                     /locus_tag="RH058_002733"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11021..11023),aa:Phe,seq:gaa)
     gene            complement(11163..11738)
                     /locus_tag="RH058_002734"
     CDS             complement(11163..11738)
                     /locus_tag="RH058_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3138302.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(11775..13472)
                     /gene="dsbD"
                     /locus_tag="RH058_002735"
     CDS             complement(11775..13472)
                     /gene="dsbD"
                     /locus_tag="RH058_002735"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV3138303.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIMLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(13448..13786)
                     /gene="cutA"
                     /locus_tag="RH058_002736"
     CDS             complement(13448..13786)
                     /gene="cutA"
                     /locus_tag="RH058_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV3138304.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(13902..15203)
                     /gene="dcuA"
                     /locus_tag="RH058_002737"
     CDS             complement(13902..15203)
                     /gene="dcuA"
                     /locus_tag="RH058_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV3138305.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(15321..16757)
                     /gene="aspA"
                     /locus_tag="RH058_002738"
     CDS             complement(15321..16757)
                     /gene="aspA"
                     /locus_tag="RH058_002738"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV3138306.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17094..17570
                     /gene="fxsA"
                     /locus_tag="RH058_002739"
     CDS             17094..17570
                     /gene="fxsA"
                     /locus_tag="RH058_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV3138307.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(17586..18842)
                     /gene="yjeH"
                     /locus_tag="RH058_002740"
     CDS             complement(17586..18842)
                     /gene="yjeH"
                     /locus_tag="RH058_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV3138308.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            19118..19411
                     /locus_tag="RH058_002741"
     CDS             19118..19411
                     /locus_tag="RH058_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV3138309.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            19455..21101
                     /gene="groL"
                     /locus_tag="RH058_002742"
     CDS             19455..21101
                     /gene="groL"
                     /locus_tag="RH058_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV3138310.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            21239..21592
                     /locus_tag="RH058_002743"
     CDS             21239..21592
                     /locus_tag="RH058_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV3138311.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(21785..22654)
                     /locus_tag="RH058_002744"
     CDS             complement(21785..22654)
                     /locus_tag="RH058_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV3138312.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         5996 a         5131 c         5671 g         5857 t
ORIGIN      
        1 atccgcgcaa gaacaaaacg gccatcaaca tcgaatacat gaaagccagc atccgtgcca
       61 gggtggagca cccgtttcgc atcatcaagc ggcagttcgg cttcgtgaaa gccagataca
      121 aggggctgct gaaaaacgat aaccaactgg cgatgttatt caccctggcc aacctgtttc
      181 gggtggacca aatgatacgt cagtgggaga gatctcagta aaaaccggaa ataacgccag
      241 aaatggtgga aaaaatagcc taaataggct gattcgatgt gtttgcggga aaaaaatcgg
      301 cccagatccg agaaatttta atcagcgagt cagcttggga agaaatgacc tgcttattcg
      361 caccttccct agtgaaatag ccaaccaatc gtccctataa ggcaggttaa atagttttaa
      421 tttagcaaat ttgataaaca actcactcgc cattaccccg attgatcttc gcatagtcaa
      481 acggcgtaat cgctctctcc cgattggcat ccagaaaatc tgcccaccat tgcagcatca
      541 gcttgcgctc atccagatgc tccgctttat ggatatacgc tgcctgcact gagttacgtc
      601 catgtggctc atctgacgtt cagccgcttt atctcgtgct gttctggtct caatacttcc
      661 atgccgttgc aattacctct taaaccattc cttatcattt tcatgtcttc gataagaaat
      721 cttcatcatt catgatcgat tttgtcgatc gattcgatcg aattttctct ttttaccttg
      781 tcttttgcat ggttttaaaa tgagcaacct tctttatcgg gtggggtaag aaccaacgga
      841 gatgattgaa atgaaaaagg ttattgctgt ttcaacactt gcaatggcag gcatgttttc
      901 ggcccagact ctggctgatg agaacaaaac tggcttttat gtgaccggta aagccggtgt
      961 ttctgttatg tcactttcag agcaacgttt cgttgatggt gaaggggcat gggcggataa
     1021 atacaaaggc agtgacaaaa gtgatacggt ttttggcgca ggccttgcgg tgggatacga
     1081 tttttatcag cactacaatg tgccggtgcg tactgaagtg gagttttatg gccgcggtaa
     1141 cgctgaatca aaatatcgtc tgagttattg ggaaagtgcc ggtggtgccg agttcgatga
     1201 tgcacagaac aagctcagtg taaacacgct gatgctgaat gcgtattatg atttcagaaa
     1261 cagcagtgca ttcacgccat ggatatctgc tggtctgggt tatgcacggg ttcatcataa
     1321 aacttcgtac atatataccg ataacagccc ggcaggcagt gaagtttatt cagcttcagc
     1381 gtcaaaatac gaaaacaacc tggcatggag cctgggtgcc ggtgttaaat acgatgtgac
     1441 gcaggatttc agccttgacc tcagctaccg gtatctggat gcaggggatt ccactctgac
     1501 ttataaagat gaggatggtg ctaaatataa atcctctgtt gatgtcagaa gtaacgagtt
     1561 tatgttaggc gcaacgtata acttctgaca gccatttcta cctgcgcttt ggggcacagt
     1621 aaagtgcagg tatgacaaat tgtcaacaac agatatcccg atgatattct ccctctggca
     1681 actaataatt ttagatgaat gaaatataaa tagagtaaaa atattactcg cgttgatttt
     1741 ttacagaggt gcaggtgata cgcagtatgg ttgtcagtct gttaaatgaa aaaggactgt
     1801 tgttgggaca cgttgttttt tgtcatatca gcccttccgg aaacaataaa ccgtattacg
     1861 aaggaggctc ccggacatct ggtttaaagt ttgaacataa tcaaataaaa atctgttgcc
     1921 ggagggcgta gttttgtgga actgacaaat gatgtcagga gtatttatat aattcactta
     1981 cacaagagta atactacagg tgcagtgatt tatggttgac tcaacgagaa atcgctcagc
     2041 ggagcgtttg attgatatcc ttgttgaatt acaaaattat ggcgtggtca gtcgctataa
     2101 tttgatgaaa aaatacaaca tcactgagcg aacagcatat cgtgatctga atatgctttc
     2161 tccctttatt gaagcctgtg gcgatggaaa ataccggctt atttcagcaa gagcaggaaa
     2221 ccagagcaaa gaatcactgc acaaatctct ggccaggtta ctggatactg atgcgatttt
     2281 tcctgaacgg gatgaaggtt tctggcagaa attagaaaac agggcaaccg aaaagcatat
     2341 tcgtgttcag tttcataacc ctgagcacac tatcagggat gatttaagaa aatatcttga
     2401 tgttcttgaa aaagcgatct gcaacagtaa tgtttgccag attgcatatg ccggtaagac
     2461 cagaatcgtt catccctata aattaactaa tcagagaagt atatggtatt tgctggctac
     2521 tgaagagaac aagttaaagt cgttttcatt agctaaaata aaatggctgg atataaaaaa
     2581 agaaaagttt gcaaagagtg atgaaataca gtcactagta agtgagtcat gtgacccgtg
     2641 ggtgagcgac aaaacatttg atgttgtgtt gttcatttac agtaatatcg cccattattt
     2701 tttgcgacgg gacttacttc cttaccagca attgctacac aaacatgaca atggcattac
     2761 gctgtcatgc aaggctgcac ataaaaatca aattgtcccg ttgattttat actggctacc
     2821 gaatgttgaa attattgaac cagtatggct gaaagaagcc gtgttaacaa tgttaggaaa
     2881 ataccttact gctgagaatg tttcctgaaa tatttcagtt gactgatgat aaagcaatct
     2941 gtacaggagg aaaagaatgg cgttaccttt tattattttg ggtgtggctg ccggcgctgc
     3001 ggcattcggc ggaaaaaaag cttacgatgg atatcaaaaa aagtcagaag cagaggatat
     3061 cctcgaaaaa gcaaaagagc gttacgagga gcataaagaa aaatttgagc aggctgaagc
     3121 tcagacatca tcaaaattaa caacgctcgg aaataaagag cttgagattg gtaaagattt
     3181 cagtgagttc gacaggattg ctgctgagct cctggcgcgt ctggaaaaag agggcaataa
     3241 agacctgaaa ctgagtgtcc cgcaacataa catcaataag attcagaacc tggccatttc
     3301 agcgacagaa tatctcagta cggtcgttgc tgccggagtt tccggggctg ctgcgggttt
     3361 tgctgtgtat agcggagtga tggcatttgc tgcggcttct acaggtacac ccattgcagc
     3421 gctttctggt gccgcggctt ataacgcgac gatggcggca attggagggg gctctctcgc
     3481 ggcagggggc atgggaatgg ccggcggcgc aatggtatta ggcaccgcag tggctgcacc
     3541 gttgattgcc attgcgggct gggcttacga cagacatgcg acgaaagctc ttgaaaatgc
     3601 ccatcagtgt gcctgcgatg ttgcacgggc agttaagaaa atgaatctgg cccgggagca
     3661 ttttgcaaaa gttaatcaat acgttgatga aattctcgct gcactgaaca gaatgcatgc
     3721 tgtatttatt aaacactatt ttgagccgct taaatccatg cattctgtta ttacagaaca
     3781 acaggatatc agtgtatcag atgaagtcat tgtgcttatt gaccatggat attcactggc
     3841 cgctattatg acggatatta ttacaacgcc actgtttaaa ccgcggaaac aggaaaatgg
     3901 tgaggcagtg ataaaagata acgttattga aatggaaacg gatgataatg gaatgaacgt
     3961 tattaataag gatgagctgg atgatgtact ggccagttcg gttgtgaaat ttgatgattt
     4021 ttcatcccag agttaatgat ttaccgggcg ttaaagcccg gctttacaag aagtgaaata
     4081 acatgaatgt ggaaaaattt gactggacag aagagttaca tcagaccatg gtgaagagcc
     4141 tggttacatc attcgggctg gattttctgt tgctaaatga tcgtaagggt ggtgatgttg
     4201 acactatcca taatgtgcgt aatggcattt atgccacgga ggctgagcga cagcggtatg
     4261 aaggtcgtgg cgaatatgat tctcatcatt accacagcca tgagaactat atcgcgacga
     4321 accgggaagg aaagcgtgcg catcaggcag ggactcttac tgatgcatat acaggcgagg
     4381 tgtttgcttc tgacgacaaa aaaaatctgg atcatattat ttccgctaaa gaaattcatg
     4441 atgatccggg gagaattctt gctgaacggg atggtgcaga actggcaaat gacgcttcaa
     4501 atctggcatt cactcatgag tcgctcaata aatcaaagaa agcagactca atggatgcgt
     4561 ttatttgcac gctgcagaaa aacagagaag atacgttaag gcagatagca gagcttgaat
     4621 ctcaggcaac gctgacagaa aaggaaaaga aacgtctgat aagtctgaga aaaaaagctg
     4681 atgcagatac tgagcgtatg gaaagggctg ataaatatgc ccgggaaaaa tatgaggcca
     4741 cgattaatca gagctattac ctgagtagta agtttgctac tgctgtcgcc agtgcttcgc
     4801 ttaattccgg ttttcgtatg gggacgcgac agatgttggg gcttattctt gccgagatct
     4861 ggttcgaact gagggaacga atcccgcata ttatcaggca gcaacggaac tcattcagcg
     4921 ccggcaaatg cctgtccgaa ataacgtcag cattacgtgc ctgccaggaa cgagtacagg
     4981 aaaaattcag ttctttttta attgccttta aagatggtgt catcggaggg atactgtcag
     5041 gtattaccac aacgctgtta aatatattcc tgacaacgga aagaatgatt gtccggctta
     5101 tcagggaaat gtggaacaat ctcgttcagg cctttaaagt cctggtattt aatccggagg
     5161 gattatcgcc ggggcagtta gccaaagcgg tcactaaact gatagcagca ggtgtggctg
     5221 ttgccggagg ggtgatgata aatgaggcga tggcgcagat ccttattttc ccatttggtt
     5281 cagagcttgc tgccttttgt ggtgcgctga cgacaggtat tctcgggctg gtgatgaatt
     5341 attatctcga atatagcgag gttatgcagc gggtatgggc atttctggac aaatttaagg
     5401 acaaattcca gcggtcactg gaatactttc aggcagtcaa tgctgaactt gatcgctatc
     5461 ttgtggaact tactgcactg gaattttctg ttgatgctgc cgagttaaat catttttcac
     5521 ttcatctgga tagcgtaaac agtgagatag agcgcggact tctgctgaga gaagaagtcc
     5581 gccgacgagg gattgcgttg ccgtttgagt caggaaatgt ggcttctgtt cgtgactggt
     5641 taaataagcg atgaaattac accctccatt tccctgtaac cagtgtgggg cctgttgtcg
     5701 tcatgtcaac agggcgggtg aaacgcaatg tctggatcgt ggtgacggta tttgccgtca
     5761 ctatcagact gattctcatt tgtgtgcaat ttatgataaa cgcccacaga tttgccgggt
     5821 tgaagaccag tatttactga attatcagtc tcagtacagc tggcaggagt ttattgcatt
     5881 aaaccaggcc gcatgcctta ttctgaataa actgtaatgc cattaatggc ggcgtcagga
     5941 cagaaactga gagaacataa aacagtaaaa tgtcatacca ttcagcagcc agcgttactt
     6001 tccccggctg ctgttattgt aaggcatttt cgttaagaat acagaaggag aggagcgtga
     6061 gattgctgtt aaaagggctc gtcggaatgg cgctggcctg gcttgttatt ggtttcggga
     6121 tggagttatt ttttctcgcc gatcgggttc atccggcatt aagagtaatt gttgtcctgg
     6181 ggatgttatt cctgctgata atgattgcgg gtggtatgtg ccgttatctt ctgtcacgaa
     6241 agaaaacatc atgacgatcc tgtttacgcc gctacatttg tggcggcgga tgatgcccgt
     6301 tttatgatta ttcagaaaaa tgtattcatc tgccacggta tgtttcacgg tcagcgccgg
     6361 gcagaaatat cgcttttact caattattat gaaaattaat cttccgttca tacactggtc
     6421 acggattagc aatgataagc gcctcgtgtt atcagtggtt gtctttaccc tttctaatat
     6481 ccttgtatcc tcaggagcgc atctgattta ccggatatta ttcttcaccc tggcgcttct
     6541 tattattaag gcgatgaaat cgcttacgct gcgttttatt cttgcctttt cgttcacatt
     6601 gctgacagcg gcagatatca gtatcagttt gtattcatgg tgtacattcg gtacaacatt
     6661 taatgatggt tttgcaatca gtatccttca gacagatcct gatgaagtaa taagaatgtt
     6721 caggatgtat gttgtttatg ttatcgcatt tattatcctc tttttattgt tcttctgttc
     6781 agcaataaat aaaacgtcct cattgccatt ggaaaaggtc acggtaatta catttttatt
     6841 gttaattaca gtaacgttat attcttcctt ccagtttgcg ttgaagaaac agtatcaaat
     6901 aaatgaagtt gatccttaca tagtcgcttc ccggtttgcc acatataccc ctttctttaa
     6961 tctgaactat tttgcactgg ccgcaaagga gcatcagaga ttaatgacga ttgctgatac
     7021 catacctcac tacgatctgg tgattacgga taacaacact gacgtatttg ttctggtgat
     7081 tggtgaatct gcaagggctg ataacatgtc catttacggg tattcgcgcc caaccacacc
     7141 agagcttcag aaacagaaat cacgactgaa actgttcact caggcaatca gcggggcgcc
     7201 ttataccgcc cttgcggtac cgctggcatt atctgtaaat accgtgctgc atcatgatgt
     7261 tcgccattat cccgataata ttatcaacat ggcgaatcag gcgggattcg atacctggtg
     7321 gctcagcgca caatcggcgt tccggcagaa cggaacagct gtggccagta ttgccatgcg
     7381 ggccagaaac agaatttatg tcagaggcta tgatgagtta ctgctcccac acctggctga
     7441 agcactgaac agtaatccgg gaggcaggaa actgattgtt ctgcatctga cgggcagcca
     7501 tgaaccggtt tgcagtaact ggcccagaga taaagccgtg tttaaaccgc tggatacaga
     7561 agaagtctgt tacgataatt ccattcatta taccgacagt ttgttggggc aggttttcgc
     7621 catgctggaa acgcgccggg catccgtcat gtatttttca gatcacggtc tggagtatga
     7681 cccgacaaaa gaacatgcct attttcatgg tggaataaaa cccagccagc aggcttatca
     7741 tgttccgatg tttatctggt acagcccggc gctgggagag catgtcgatc gccagacggt
     7801 gaacagcgtt ttttccactg cgtataatga ttatctgatt aatgcatgga tgggcgtaac
     7861 cagaccggct caaccgaaaa cgccggagga ggtgattacc cgctggcagg gaaaatccgt
     7921 ggtttttgat gccagccata acgtgtttga ctacaaagta ttacgaaaga actttaatga
     7981 acccctggaa taattcgtta aagtaattta ctacgctatt ttttcaaagg aaactttcag
     8041 aaaataaccc cagggcgata acctggggaa gtggggggct gtgttattac tggtggataa
     8101 tttattactt gtttgaaaga tacactaatg caatatctac cgatgctgga tgcttgtgtg
     8161 tttcattcct gccgctgaaa acggcaatat ccagggccag acatattcac cgttaatatt
     8221 tctgcatttc tgtacaattt cctcagaaag tgtctcatca attctttccc gcaacagctt
     8281 ctgctgtctt aaaatgcttt cgcatatttc gaaatcatct tcctgcatca gcatttcggc
     8341 gcatttaatg atttctgccc gttcatgcag cacagaggaa tggttataga gccagtttac
     8401 aaacagcaat gcttccggat attgtcccca taatggtgag tatgttgcat aataatcttc
     8461 ggcagtatcc ggcccggcag aatagttatg ccatacagca agaggaaatg gatgcagatt
     8521 tccgcacaga atttctgcga tgtaagtatt tgccgcaaat ccgcgacgaa atgcagttgc
     8581 agcttttacc agttccccat taagaaaatg cagaagacca agttcatacc agtaaggtga
     8641 aaattcatca gcatgttttt tcagtacact gaaggctcgc tcatgatcac ctgttcgtaa
     8701 cagctctgaa cccaacaacc agcgggcacc ctgattatct tcaggattgt atgccagtat
     8761 tttatcagta agcataaccg catcctgatg acgctgtaag tgcacatttg cgagaattgc
     8821 tgcataaagc gcgcgcagat aaggccggtt ttcgggatgc atccagatga tttcaccact
     8881 gaaactttcc gggatgagac gattacctgc cgccagtcct ttaagagcgg cgttcagtgc
     8941 cttacgcggg gcattctgct ccagaaatgc atatgccaga tgagcgtgta tatcaataaa
     9001 gtcaggctcc tgttgagcaa ggcgtctgag tttgttgatg taactcgtct cgctgatttc
     9061 atcagtgtcg tatgcatcga gcagttcttc aaactccccc agtaatgggc caaaccagat
     9121 atccggaaat acgaaacgcc cctggttatc gttcacttca aagcgtaatc tgtcgttcat
     9181 tttctgagcc tgtaaaacgg atgggacaca atctgaaaag cataaaagtt gtgatgttgt
     9241 gtatcaagtc ttgtttttgt ccgggatgaa tgttgtttac aatatcatcg gcagtggttt
     9301 ttcatgagaa tgaaaattta tctttatctg cattccggtg tattgtgaat tgtcggctga
     9361 aacagcatat taatcgattt ttttagggga atggtgctga aataacgaca tgatgatgat
     9421 ttatattaca ggtatcaaaa ttgttagcgg tccataacgg tgtgatgttt tgagattgtt
     9481 tacagatgca atattaaata ttttgtcaaa aaacaaaaaa tatctaaaaa taaagcatga
     9541 aaacaaaatt cctccggacc tggaggccgg aggagaactt gtggattgag agaaaatatc
     9601 agaacaagtg ttcttaaccc tgactctctt gcctggaaag cgaatcaatg taatctgcgt
     9661 accattgcat catttcacgg cgaccatcga gatattgggc atgattatag gtacccctga
     9721 tggcgttttt atccacatga gctaactgca tttcaatcca ggcagaatta aagccctgtt
     9781 cgtgcagaat ggtactcatg gtgtgacgaa aaccatgacc ggttgccctg ccatcgtaac
     9841 cgatacgttt gagcaccatg ttgatgctgg cttcgctcat tggcttgttg atatcacaac
     9901 gtcccggaaa aaccagctgg tattgtccgg agaaagtttt gagctgcagc agaatgtcag
     9961 tggtctgtcg tgacagtggt acgacatgtt tacgtcgcat tttcatgcgg ctttcaggga
    10021 ttgtccacag ggcattatca aaatcaattt ctgaccattc agcggaccgt agttcaatcg
    10081 ttctgaggcc tgtcagaagc agcagatttg tcgccatgcg ggtaacagga ctgccatgat
    10141 attcagcgag cttccggaga aaaacaggga gctcatctgc tctcaggtgg gggaaatgtg
    10201 cagcttttgg tgccgccagc gtactggtca gttcagatgc cggattgaca gtggctcgtc
    10261 cggtggcgat ggcataacga aaaatctgat tacagtattg gcgtacctta cgcagtttct
    10321 ccagcgagcc acgtttttct atgcggcgca gaatttccag catttccagc gggaggatat
    10381 ccgtaacgct tcgtttgccc agatacggga aaatatcttt atccagcgct tccagtacgc
    10441 ttctggcgta actttcagac catgacaaag atttatgctg gtgccattcc gtagcgaggg
    10501 catggaacgt attggtggca gagattttct gagtgagttt ttcctttcgc ttcttttcgc
    10561 ccgggtcacc accttcggag ataatttttc gggcatcgtc gcgtttgatt ctggcttcag
    10621 caagggagac gtcgggatag acgccgaggg ccagtttttt ctcttttcct gctatccggt
    10681 atttaagccg ccagtagcgg gcacccgtgg tttttaccag cagatataaa ccacgttcgt
    10741 cagagagttt atagtctttt tctttaggtc tggctgtttc gacctgtctg gctgtaagtg
    10801 ccatatgggg gctcctgaaa ctgaccgaac acaatgagcc cccaccggtg cccccaatga
    10861 acgcttgatt gagagagagt tcagtagagt tcggtggagt tcaggatgtt cagattgctt
    10921 gtaacgatgt gattatactt gagtttcgtg gagttcggga gatgaatctg gaagtgaaag
    10981 tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc tgctctaccg
    11041 actgagctat ccgggcaacg gggcgcatta aaccgtaatc tgcacatctc gtcaacctaa
    11101 tttcagtaaa agcgattcaa ctgcttaaga ttgcggcaaa tcgcttcttt tctgtgtttc
    11161 tgtcaggtca atgcaccacc ctggcggcag cgggcgaagc gcaggatatc ttccgccagc
    11221 cgatgtgcag tgtcgacatc cgcctggcta cgattcacca gcattcggct taaacagcct
    11281 tccagcacca gttccatctg ctttgctacc atcgccggat cgtcaacttc cagtgtggtt
    11341 aacagttcgt gggtgaaatc gtaggccgcg cttttttgct gatcggccag ttgatgaata
    11401 gggtggccag gatcgggata aaacgtacag gcagcgataa atagacagcc cggatagcgg
    11461 ttgtttttaa cgcactccga taacgcctga taacgtgcca gcagcttttg ttcggcggtt
    11521 tgcgtttcgt ccagcatcag ctgacgacgc cagacatcta tctgttggct aagataacgc
    11581 agcgcatcgt agaggattgc ctctttgtct ggccagaagc ggcgtagctc gtccagtgga
    11641 taatccacac gttcagcaac catctccagc gtggtgttgg caatcccttg taattctaat
    11701 aatttcaggg cttctcccag tacatcttca cgttgcacgc tattttcctc cgtctttccc
    11761 actgcaagtg tcgttcacgg ttggcgatcg cgcaaatgtg cgctgaaggt ttcagcatcc
    11821 ataaagcccg tgacgcgtgc ttgtggatgc tcctggcctt gtccgtcaaa aaagagaatt
    11881 gtcggtaggc caaggacatt aagatgcttt aacagcgcca catcttgtgc gtcgttggcc
    11941 gtgacgttgg cctgaagtaa gaccgtgtct gctaacgctt tttgcacctg cgggtcgctg
    12001 aaggtgtatt tctcaaactc tttacaggcg acgcaccagt cggcataaag atctaacatc
    12061 accggtttgc ctttggcttc aacgagcgcc tgatttaact catctaccgt tttgatttgt
    12121 gtaaagttga gatgcgtctg agtttgcgcg gtatgcgtcg caccaaatgc ccaatcctga
    12181 agtgggcgca cgctaaccaa tgccgctgcc agcaggatta tttgcaccac gcgcatccag
    12241 ccgcgtttag cctgtaggct ggtgataaag gcccagccaa agaatgcgac acccagcgcc
    12301 gaccacaagc gtaatcccca tacatcacca atcactcgct ccagcaggaa gaccggcagt
    12361 gcgaggatca caaaaccaaa cgcggttttg acttgttcca tccacgggcc gcttttcggc
    12421 agcaagcggt taccaaagac ggtaattagc atcagcggca ggcccatgcc caacgcatag
    12481 agataaagcg tgccgccgcc cagccacatg ttcccgcttt gggcgatata cagcagaatc
    12541 gcgctaagcg gtgcggtggt gcatggtgaa cagatcagtc cggcaatcgc ccccataaca
    12601 aacacaccgc caggtgagcc gccctgttgg cgattgctca tcaacgtgag acgtgtttgc
    12661 agcgaagagg ggagttgtag agtaaacaag ccaaacattg acatcgccag caaggtaaag
    12721 acgatggcga ggccaatgag cacgtatggg tgctgtagcg ccgcctggaa ctgtaaccct
    12781 gcggcggcaa ctaccagacc cagcgccgtg taagtcagtg ccatcccctg cacataaata
    12841 aaggtcagca acaatgctct ggcagtggag agccgctgtt taccacccag catgatgcca
    12901 gaaatcagtg ggtacattgg cagcacgcat ggcgtaaagg cgataccaat accgatcaac
    12961 aacgcccaga gcgcggaaaa gggcaattgc gcggtgtgct gctcttgctg cgaaacagac
    13021 acaggctgtg acgctgcgtt gttggcgacc acttcgctta acggaacggt tttggtttct
    13081 ggcggataac agaaaccggc atcagcacag ccctggtagg tgacagttaa cgtcgctccc
    13141 gcactcgcct ggttgatggt tacgggaagc gtcagccgat cgcggtaaat ctcgcttttg
    13201 ccgtaaaact catcttcatg ccagacgcct tgcggcagct gcacgtcggc aattttcgcg
    13261 tgttccggcg taatgcggat ctgtttacgg tagaggtagt aaccgtcttt gatctgccag
    13321 gtcagattaa ggtcatgttg gttttgctga aaatcaaaag taaaggcttg atccgcgggg
    13381 acaaattgtg aacgtcccgg cgcgtcgaat aatccggcaa aaacggaagt gctgcaaagt
    13441 agcaggatca gcgtaaagat gcgttgagcc atgagaggta atctgtgtct ccgtgtgtaa
    13501 caggtaaaac cagaagttcc ggggtttgat atggatgatg agacttcagg cattccagca
    13561 gtgcctgctg gtgagatacg gtagttttta aaatcatctg cacttcgtat tcttgctcca
    13621 gcttaccttc ccagtaatag agagaggtag cgccggggat caaggtcgcg caggccgcca
    13681 gtttttccgc cagcactttg gcggctaaat cctgggctgt cgcttcatcc ggtgcggtac
    13741 atagcaccac gacagacgcg gtattcgaac ttttttcatc aagcataaac acctcgcaag
    13801 aacagatgga accgcaagag agaggtcact atacaacgga cgggggaagg atgttagtcg
    13861 gcgggaaaga aagcggggcg tgaacgcccc gcgattggtc attacagcat gaagctaccc
    13921 agcacgaagc cgaagcaaac ggccagggca acacccagag tacccgggat gaagaacgga
    13981 tggttgaaga cgaatttacc gatacgggtt gtacccgtgt catccatctg taccgcagca
    14041 accagcgtcg ggtaggtcgg cagaatgaac agaccagaca ccgcagcgaa agaagcaaca
    14101 gcggtcagcg gtgaaacgtt cagtgccagg gccatcggca tcagtgcttt tgcggttgca
    14161 gcctgagagt acagcagagc agaagcaaag aagaagatga cggccagcag ccacggatga
    14221 ccctgaatca cttcaccagc ggtatctttg atccagtcga tgttgttgga aacgaaagta
    14281 tcgcccagcc acgcaacacc caggatacaa atacaggcgc tcatacctgc tttgaaggtg
    14341 ctggagttga ggatgttgtc ggtatcgact ttacagataa cggtggtcag agttgcaacg
    14401 ctgagcatga tgatcaggat tgcgttggtg gtgttcatca gcggtttttc aaccagaccc
    14461 atgcttgggc tgttgatgat tgcatagata accacgccaa ctacgcccag caggaacagc
    14521 caaacggacg tttttgcacc ggatttgatt tcaatctgct tttcaccgcg cagttcaacc
    14581 aggccctctt ccagacgctt gcgataaatc ggatcgtcag agagtttgga gttgaacagc
    14641 atagtgacca gaaaggacat caccagaacc gccagcaggg tggacgggat gaccacggag
    14701 agcagatgga ggtagctgat gccatgacct tccatcacgg aagacatgta aaccactgcc
    14761 gctgagattg gcgatgcggt gatcgcaatc tgcgcggata ccactgcagt agacagcgga
    14821 cggcaaggtt taacgccttg ttccttcgca acttcagcga taactggcag tgtcgccaga
    14881 gagatgttgc cagtaccagc aaagattgtc aggaaatagg tcacgatagg tgcgaggatc
    14941 gtgatgtatt tcgggttacg gcgcagcagc ttttctgtct gatgaaccag atagtccaga
    15001 ccgccagcaa cctgcatggc agaaatagcg gcgataaccg ccatgataat ggaaatgaca
    15061 tcgaacggga tgttaccggg tttaacgcca atagcggcaa gaaccagcac ccccaatccg
    15121 cctgcaaaac caatacctat tccccccaat ctggcgccca agaagatcgc cagcaaaact
    15181 atgatgagtt ctacaactag catattagcc ttccttgttt tttaacaagt tgatattaga
    15241 ttgttgtttt taagttactg ctcacaagaa aaaaggcacg tcatctgacg tgcctttttt
    15301 atttgtacta ccctgtacga ttactgttcg ctttcatcag tatagcgttt tgctttgtaa
    15361 gccgggtgca tcagattctg tacggagaaa atatcgtcaa gttccgcttc agtcaacaga
    15421 ccgcgttcca gaacgacttc acgtacactc ttaccggttt cggcacagat tttacccacg
    15481 atgtcaccgt tgtggtgacc gatgaacggg ttcaggtaag taacgatacc gatagagttg
    15541 taaacgtaac cttcgcacac ttctttgtta gcagtgatgc cgttaatgca tttttccagc
    15601 aggttgtagc aagcgttggt cagaatgtga acggattcga acatggcctg gccaatgacc
    15661 ggctccataa cgttcaactg cagctgacct gcttctgctg ccatggtaac agtggtgtcg
    15721 ttaccgatga ctttgaagca tacctggtta accacttccg gaacaaccgg gtttacttta
    15781 gctggcatga tggaagagcc cgcctgcagt tccggcaggt tgatctcgtt caggccggca
    15841 cgtggaccag aagagagcaa gcgcaggtcg ttacagattt tggacatctt cacagccagg
    15901 cgtttcagcg cgccgtgaac cataacataa gcgccgcagt cagaggtcgc ttcgatcagg
    15961 tcttcagccg gtacgcatgg gaagccagtg acttcagcca gttttttcac tgccagcgga
    16021 gagtactctt tcggcgtgtt cagaccagta ccgattgctg ttgcgccaag gttaacttcc
    16081 agcagcagtt cagcggtacg ttggatattt ttcacttctt ctttcagcag gatgctgaaa
    16141 gcgcggaatt cctgaccgag ggtcatcggt actgcgtcct gcagctgggt acgacccatt
    16201 ttcaggatgt cctggaattc gacagcttta cgttcaaagc cttcacgcag ttggttaatc
    16261 gcatctacca gcttaatcag ggaagagtaa actgcgatac ggaaaccggt cgggtaggcg
    16321 tcgttagtgg actgacattt gttaacatgg tcgttcgggt tcaggtactg atattcacct
    16381 ttctggtgac ccatcagttc cagaccgata ttggccagca cttcgttggt gttcatgttt
    16441 acggaagtac ctgcgccgcc ctggtagacg tctaccggga actgatccat gcattttccg
    16501 ttgttcagga cttcatcaca tgcggcaatg atggcattcg ctacactttt aggaatggtt
    16561 tgcagctctt tgtttgccat agctgcggct tttttaacca ttaccatacc gcgaacaaat
    16621 tcaggaatat cactgatttt gttgttgctg atatagaagt tttcaatcgc tctcagagtg
    16681 tgaacaccat agtaggcatc agctggaact tccctggtac ccaacagatc ttcttcgata
    16741 cgaatgttgt ttgacatgtg aaccttcttt ttcaagctgc caatgatttg ctttaaacac
    16801 acagaatata tgtggtttcg aatgtttttc gaccgacgat tatcccctgc atcgaccgaa
    16861 tacccgagat catatgctgc ttgaggattt ctaccgtaat ctggatcact ttaagtgtcg
    16921 gtttttaccc cttaattatt aatttgtgaa atagatcacc gctttgggat tactaccaaa
    16981 aatagttgcg caaacatctt gaaattttgc taatgaccac aatataagct gaacgcgatt
    17041 cgcaacccat tcaggtagcc gggttttacc ggctgctatt acaggagaaa cctttgcgct
    17101 ggttaccttt tattgccatt ttcctttatg tctatattga gatttcaatc tttattcagg
    17161 ttgcccatgt attgggggta ttgctgaccc tcgtgctggt tatattcacg tcagttatcg
    17221 ggatgtcact ggtacgtaac cagggcttta agaatttcgt gctgatgcag caaaaaatgg
    17281 cggcgggtga aaacccagcg gcggagatga ttaaaagtgt ttcgctgatc attgccggtt
    17341 tgctgctttt attaccgggc tttttcaccg acttcctcgg tcttctgctt ttattaccgc
    17401 cggtgcaaaa gcatctgaca gtgaagttga tgccgcattt gcgcttttct cgtatgccag
    17461 gcggcggttt tagcgccggg accggtggcg gtaatacttt tgatggtgag taccagcgaa
    17521 aggatgatga gcgcgaccgc cttgatcata aagacgatcg ccaggattaa tgtcgaaacg
    17581 ccggattatg tggttatgcc attttccggc gtttttcgtt ttggcagcaa cagccataac
    17641 cccgccagca tgatcagcgc atagagactt ttccagccga ccattgccag taacagaacg
    17701 cataacagcc cgccaaccac cgccagtagt cgataacgtc cttgcaataa tttacagcct
    17761 gccagcatgc ataacagata aatcataata aagatgccat tggcataaat aataagagcg
    17821 tccagattga tctctaaagc atgaatcacc aacgtgctca ccacacagca gccgagcacc
    17881 gcattgaggg cattattcgg gatatggcga gaagagaggc gtgccaggta gtggtcagga
    17941 ttatgttgcg cctgcgacca gaccaggcgg gcgaagctct gtatataaat gttgagactg
    18001 gcaaagcagg ccagatagcc aatcacgcag gcaatccata acgctcctac accgaacaac
    18061 tgcactacaa tttttggaag cgatgctgcc gccgccattt tttcaccata ggcgtcgaag
    18121 tgtaagacga ctaccgtaca gccccagtag actaatcctg ccagcagcag accaatcatc
    18181 aatgcgcgag gaaaatcacg ctctggattt ttaaattccg aggcaagatg ggcaaatgct
    18241 tccagaccga caaaacacca gaacatcact gataacgcag caaataaccc ggtaagttcg
    18301 atattaccag gtgccgggaa ggggatattc gcaggtttga tatcgcccgc ccaccagata
    18361 gcgacaatca gcgcgacgat aagtccggca ataacggttt gtagattagc actggaactg
    18421 gcaccgcgag taccgatata ccacaccagc gccagcgtac cgagttctgc caacaacagt
    18481 tgccagctat gccagccaaa cattgcctgg ccgaacccgg cggcaatttg tagcgcggca
    18541 ggcaaaccca cgggaatgac cgataaaaac aaccagccgg tgactcgctc aagtcgcgaa
    18601 ccaaacgcca taccgacgaa gtgcgcgaca ccgcctgcgc tgggatagtg gcgacccaga
    18661 atcgcaaaca caatcgcaat cgggaacact aagataatca aaacgggcca cgcccacagg
    18721 ctgttattgc ccgctaccag cgcagctaac gcaggaacgg caaacacgcc agtgcctaat
    18781 aatgacgtcg atagcaggcc aatgccttgg gccagcccca gttcttgttt gagtccactc
    18841 atgggttgat atccgattgc gcccaaattt tgggcaactg cgtagatttt cgatggtagc
    18901 acaatcagat tcgcttatga cggcgatgaa gaaattgcga tgaaatgtga ggtgaatcag
    18961 ggttttcacc cgattttgtg ctgatcagaa ttttttttct ttttccccct tgaaggggcg
    19021 aatcctcatc cccatttctc tggtcaccag ccgggaaacc acgtaagctc cggcgtcacc
    19081 cataacagat acggactttc tcaaaggaga gttatcaatg aatattcgtc cattgcatga
    19141 tcgcgtgatc gtcaagcgta aagaagttga aactaaatct gctggcggca tcgttctgac
    19201 cggctctgca gcggctaaat ccacccgcgg cgaagtgctg gctgtcggca atggccgtat
    19261 ccttgaaaat ggcgaagtga agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa
    19321 cgatggctac ggtgtgaaat ctgagaagat cgacaatgaa gaagtgttga tcatgtccga
    19381 aagcgacatt ctggcaattg ttgaagcgta atcctcgcac gacactgaac atacgaattt
    19441 aaggaataaa gataatggca gctaaagacg taaaattcgg taacgacgct cgtgtgaaaa
    19501 tgctgcgcgg cgtaaacgta ctggcagatg cagtgaaagt taccctcggt ccgaaaggcc
    19561 gtaacgtagt tctggataaa tctttcggtg caccgaccat caccaaagat ggtgtttccg
    19621 ttgctcgtga aatcgaactg gaagacaagt tcgaaaatat gggtgcgcag atggtgaaag
    19681 aagttgcctc taaagcgaac gacgctgcag gcgacggtac caccactgca actgtactgg
    19741 ctcaggctat catcactgag ggtctgaaag ctgttgctgc gggcatgaac ccgatggacc
    19801 tgaaacgtgg tatcgacaaa gccgttaccg ctgcagttga agaactgaaa gcgctgtccg
    19861 taccgtgctc tgactctaaa gcgattgctc aggttggtac catctccgct aactccgacg
    19921 aaaccgtagg taaactgatc gctgaagcga tggacaaagt cggtaaagaa ggcgttatca
    19981 ccgttgaaga cggtaccggt ctgcaggacg aactggacgt ggttgaaggt atgcagttcg
    20041 accgtggcta cctgtctcct tacttcatca acaagccgga aactggcgca gtagaactgg
    20101 aaagcccgtt catcctgctg gctgacaaga aaatctctaa catccgcgaa atgctgccgg
    20161 ttctggaagc tgttgccaaa gcaggcaaac cgctgctgat catcgctgaa gatgttgaag
    20221 gcgaagcgct ggcaactctg gttgttaaca ccatgcgtgg catcgtgaaa gttgctgcgg
    20281 ttaaagctcc gggcttcggc gatcgtcgta aagctatgct gcaggatatc gcaaccctga
    20341 ctggcggtac cgtaatctct gaagagatcg gtatggagct ggaaaaagca accctggaag
    20401 acctgggtca ggctaaacgt gttgtgatca acaaagacac caccactatc atcgatggcg
    20461 tgggtgaaga agctgcaatc cagggccgtg ttgctcagat ccgtcagcag attgaagaag
    20521 caacttctga ctacgaccgt gaaaaactgc aggaacgcgt agcgaaactg gcaggcggcg
    20581 ttgcagttat caaagtaggt gctgctaccg aagttgaaat gaaagagaaa aaagcacgcg
    20641 ttgaagatgc cctgcacgcg acccgtgctg cggtagaaga aggcgtggtt gctggtggtg
    20701 gtgttgcgct gatccgcgta gcgtctaaac tggctgacct gcgtggtcag aacgaagacc
    20761 aaaacgtggg tatcaaagtt gcactgcgtg caatggaagc tccgctgcgt cagatcgtat
    20821 tgaactgcgg cgaagaaccg tctgttgttg ctaacactgt taaaggcggc gacggcaact
    20881 acggttacaa cgcagcaacc gaagaatacg gcaacatgat cgacatgggt atcctggatc
    20941 caaccaaagt aacccgttct gctctgcagt acgcggcttc tgtggctggc ctgatgatca
    21001 ccaccgagtg catggttacc gacctgccga aaaacgatgc agctgactta ggcgctgctg
    21061 gcggtatggg cggcatgggt ggcatgggcg gcatgatgta attgccctgc acctcgcaga
    21121 aataaacaaa cccccgggca gaaatgtctg ggggtttttc ttttggtcat ctttctagta
    21181 taagattcag acacggacga cgcgagtggc gtccagctca ttgattatgg ggaataacat
    21241 gcacgtaaaa tacttagcag ggattgtcgg tgccgcgcta ctgatggcgg gttgtagctc
    21301 cagcaacgaa ttgagtgctg ccggtcagag tgtacgcatt gtggacgagc agccaggcgc
    21361 agagtgccag ctgattggta ctgcgacagg taagcaaagc aactggcttt ccgggcaaca
    21421 cggagaagaa ggcggttcta tgcgcggcgc agcaaacgat ctgcgcaacc aggcggctgc
    21481 aatgggcggt aacgtgattt atggcatcag cagcccgtcg cagggaatgt tgtccagttt
    21541 tgtcccgacg gatagccaga ttatcggtca ggtttataag tgcccgaact gatgtgatgg
    21601 tgacaaacgt ataattgcca gatgcgtttc gcttatcagg cctacataag gcattgcaac
    21661 tgattgaatg cttgagaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgac
    21721 caacgatcac gttatcagtc atttcaggct ggatggctcc agcctcgcat tagcaaagat
    21781 gagattattt cgcctgtggt gcagttttgc tggtggattg aacacgtaag tgatgcagcg
    21841 cgcgtaagca ttgatcctgg gttagcggtt gggcgttatt cgccgctacg gtgcgaacat
    21901 agacctgaca tggcacaccc gcgagttttt tcagtcgtgg gctgaaatca agcattcggc
    21961 gagtgatcgc ctctatttct ttacccggcg ttgcttcacg cgttttcacc actgcgccgc
    22021 taaattgttc tttgccactt tcatctttaa agcggtaaag caccgccaga taatggtcga
    22081 acaggttatg tttccattcc ggttgggaat aggtgtcata ttccagattg ccattttcct
    22141 ggcagtcaac gtcatataac ggcaggaaat ctaacagtga ggtaatacgt aatgccagtt
    22201 cacgcatttc cgcattatta attgcatgaa tgattgctcg cgccagcatt tcaatctgca
    22261 actcattgac gactaactca catttgctac cgtcatgtag cgttaatacg aaggtaagat
    22321 tttcgccttg attatcggta agttccagcg tgttaacccg acgattaata tcggcatttt
    22381 taagctcatc aaccagaatt tctgggatgt tttctgccat tttcttaatg actttatcgc
    22441 gagcttgctc atactgaagg cgtgcagcag catccagttt atgcttttga tgcatacgac
    22501 tttccagcgc aatcagcagg tcgcgcagtt ccatgacaga cataaagaac aacgattctt
    22561 tattgcgagg ttctttaatt ttcagcgcca gtgcaatgaa attattactc ttgcgaataa
    22621 caccggtatt aacacccttg atgcttatgg ccatg
//
LOCUS       DAPHDX010000073        21984 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPHDX010000073 DAPHDX010000000
VERSION     DAPHDX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.073"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(112..2181)
                     /gene="glyS"
                     /locus_tag="RH058_002745"
     CDS             complement(112..2181)
                     /gene="glyS"
                     /locus_tag="RH058_002745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV3138313.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2191..3102)
                     /gene="glyQ"
                     /locus_tag="RH058_002746"
     CDS             complement(2191..3102)
                     /gene="glyQ"
                     /locus_tag="RH058_002746"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV3138314.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3197..3496)
                     /locus_tag="RH058_002747"
                     /pseudo
     CDS             complement(3197..3496)
                     /locus_tag="RH058_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3671..4666
                     /gene="wecH"
                     /locus_tag="RH058_002748"
     CDS             3671..4666
                     /gene="wecH"
                     /locus_tag="RH058_002748"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV3138315.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4708..5145)
                     /locus_tag="RH058_002749"
     CDS             complement(4708..5145)
                     /locus_tag="RH058_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV3138316.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5191..5532)
                     /locus_tag="RH058_002750"
     CDS             complement(5191..5532)
                     /locus_tag="RH058_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138317.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5701..7155)
                     /gene="xylB"
                     /locus_tag="RH058_002751"
     CDS             complement(5701..7155)
                     /gene="xylB"
                     /locus_tag="RH058_002751"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV3138318.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7227..8549)
                     /gene="xylA"
                     /locus_tag="RH058_002752"
     CDS             complement(7227..8549)
                     /gene="xylA"
                     /locus_tag="RH058_002752"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV3138319.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8915..9907
                     /gene="xylF"
                     /locus_tag="RH058_002753"
     CDS             8915..9907
                     /gene="xylF"
                     /locus_tag="RH058_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3138320.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9985..11526
                     /gene="xylG"
                     /locus_tag="RH058_002754"
     CDS             9985..11526
                     /gene="xylG"
                     /locus_tag="RH058_002754"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV3138321.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11504..12685
                     /gene="xylH"
                     /locus_tag="RH058_002755"
     CDS             11504..12685
                     /gene="xylH"
                     /locus_tag="RH058_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV3138322.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12763..13941
                     /gene="xylR"
                     /locus_tag="RH058_002756"
     CDS             12763..13941
                     /gene="xylR"
                     /locus_tag="RH058_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV3138323.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14049..14873)
                     /locus_tag="RH058_002757"
     CDS             complement(14049..14873)
                     /locus_tag="RH058_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV3138324.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAIAAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15193..17223
                     /gene="malS"
                     /locus_tag="RH058_002758"
     CDS             15193..17223
                     /gene="malS"
                     /locus_tag="RH058_002758"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV3138325.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17401..18654
                     /gene="avtA"
                     /locus_tag="RH058_002759"
     CDS             17401..18654
                     /gene="avtA"
                     /locus_tag="RH058_002759"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV3138326.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18806..19279)
                     /locus_tag="RH058_002760"
     CDS             complement(18806..19279)
                     /locus_tag="RH058_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV3138327.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19381..20229)
                     /gene="yiaJ"
                     /locus_tag="RH058_002761"
     CDS             complement(19381..20229)
                     /gene="yiaJ"
                     /locus_tag="RH058_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDV3138328.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20430..21428
                     /gene="yiaK"
                     /locus_tag="RH058_002762"
     CDS             20430..21428
                     /gene="yiaK"
                     /locus_tag="RH058_002762"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDV3138329.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21440..21907
                     /locus_tag="RH058_002763"
     CDS             21440..21907
                     /locus_tag="RH058_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV3138330.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         5546 a         5575 c         5402 g         5461 t
ORIGIN      
        1 attaatatat gagataatct ttattcagca gaagattatt aaaggttgct gtattattta
       61 gcgataataa aaaaagcctg ccagatggca ggctttttaa taacggcgtt attactgcaa
      121 cagcgaaata tccgcaacgc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg
      181 gttgagacgc aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac
      241 cggctcacgc agctctgcca gttcgaccag cgcatcctgg taacgacctt ccgcaaagta
      301 cggctccagc ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt cttccggctc
      361 tttcagggta gaggcattca cgcggtcgct cagcacttcg tcagatttcg ccaaaatgtt
      421 ggagacacgc ttgttcgccg ccgccagtgc agcagctgct tcaagggtac ggaagtgcga
      481 taccgctttc atgcgggcat cgaaatcagc cggacgagtc ggacgacgcg ccagtaccgc
      541 ctggatggtg tcaacggtgt aaccttcgtc ctgataccag gcgcggaagc gaccgagcat
      601 aaagtcgata acatcatcaa ccacgttggc gttagtcagc ttatcgccat acagacgcac
      661 tgcttcttcg gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taattcgcag
      721 cacgccaagt gcggcacgac gcagcgcaaa cgggtctttg tcgcctttcg gatgctggcc
      781 gataccgaag atacccgcca gagtatccat cttgtcagca atcgccagcg cacaggctac
      841 cgggttagac ggcaggtcat caccggcaaa gcgcggctga tactgctcat tcagcgccac
      901 cgcgacatct tccgcttcgc catcgtgacg cgcatagtgc atccccataa cgccctgggt
      961 gtcggtgaac tcgaagacca tgttggtcat caggtcacac ttagacagca ggcccgcacg
     1021 ggttgcgtgg ttaacgtcag cgccaatctg ttcagcaatc cagccagcca gcgcctggat
     1081 gcggtcagtt ttgtcacgca gcgtacccag ctgttgctgg aacagcacgg tttgcaggcg
     1141 cggcaggtta tcttccagac gttttttacg gtcggtgttg aagaagaact cggcatccgc
     1201 cagacgcgga cgaacgactt tctcgttacc ggagataatc tgctgcggat ctttcgattc
     1261 gatgttggca acgaagataa agttcggcag cagtttgccg tcgttcgcat acaccgggaa
     1321 gtatttctgg tcacctttca tggtgtaaac cagcgcttca gccggcaccg cgaggaattt
     1381 ctcttcgaat tttgcggtca gaacgaccgg ccactccacc agcgaagcca cttcttccag
     1441 caggctttcg cttaagtcag cgttaccgcc aatcttacgc gctgcttctt cggcatcggc
     1501 tttaatcttc gctttacgtt cttcgtaatc ggcgatgact ttcccgcgct cacgcagaat
     1561 ttccggatac tgatcggcgt tgtcgatggt gaattccggt tcgcccataa agcggtggcc
     1621 gcgaatcacg cgatcggact gaatgcccag aatggttgcc ggaatgactt tgtcgcccag
     1681 cagcagggtc acggtgtgca ccggacgcac gaagtgcacg tcgcttgcgc cccaacgcat
     1741 cagtttcggg atcggcagtt tcgccagaga agtcgcaacc atattcggca gcagtgcttc
     1801 ggtgctttcg cccttcacat gggcgcgata cagcagccat tcgcctttat cggtagtcag
     1861 acgctcagcc tggtcaacgg taataccgca accacgcgcc caaccttctg ccgctttgct
     1921 cggtttgcct tcagcgtcga acgcctgggc aatcgccggg ccgcgttttt cgatttcacg
     1981 atccggttgc gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa
     2041 ccattgaacg gtgccgtgtg cgaggccagc gttatccagc tccgcagtaa aattcgcagc
     2101 aaaggactca gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac
     2161 cagaaaagtt ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc
     2221 gagggcttca cgggaagcgt agtatgcttc tgccactgct ttggtcaggg tgcgaatgcg
     2281 cagaatgtag cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa
     2341 gctgtgggcg gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc
     2401 cagcagctgc tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc
     2461 cgcgtattcg aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta
     2521 ggtggtttta cccagcgggc cgtcgctcca gaccaggtcg taaacgctgt ctacgccctg
     2581 aatgtacatg gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc
     2641 cagaccacca acctgctgga agtaagtgaa ctgcgtcact tccatgccgt ttagccacac
     2701 ttcccagccc agtccccagg cacccagagt cgggttttcc cagttatctt ccacgaaacg
     2761 gatgtcgtga atagtcgggt ccatgcccag ctctttcaga gaaccgaggt acagctcctg
     2821 aatattgtcc ggcgatggct taatgaccac ctgaaactga tagtagtgct gtaaacggtt
     2881 ggggttttcg ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc
     2941 catcggttct ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac
     3001 ttccatgtcc aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt
     3061 caggatcaag ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata
     3121 cgtgtggatt taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa
     3181 cgggaaaaag cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag
     3241 aacagacaaa ctgctcttta cggaaggtat aaccgcgcat ttcatagctt ccctggaact
     3301 gttcgaaggc ggtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctaat
     3361 ccttgcataa cgcttccata ttcaacgaac gcagaggatc aacctttacc cgttgtgctt
     3421 tctgcacggg tgacggtgta gaacaacccg ctagcaccat aagcgccatt gccggaaaga
     3481 aagcgttcat catcattaaa ttaccgtctg gtttgcaggt gagtcttatt atttatatcg
     3541 gtatagtggc tgatatctta cgaatttcct taagcctcta taagctgtag tgataaaact
     3601 cagattattc cagtgcgcca actgaatgat ttatcggtca gacttttttt aaccagatac
     3661 agagacacag atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt
     3721 aatggtggtg atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc
     3781 cgtcacctgg gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt
     3841 tttcatgata tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt
     3901 gcgtatcggc ttatgcctga ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt
     3961 tacctccatc aatatggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca
     4021 cttgtggttt ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa
     4081 gaacgtcggc ggaaaaatgt tgctggtgct aatggtggtg attggcatta tcgctaaccc
     4141 aaacacagtg ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa
     4201 tggcgatact ttttactaca ttctgtatgg catgttgggc cgcgctatag ggatgatgga
     4261 cacacagcat aaagcactgt cgtgggtgag cgccgcgctg tttgcgacgg gggtttttat
     4321 tatctctcgc gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta
     4381 tctttactgt gggccgatgg tttttatctg cgcaatcgcg ctattgactc tggttaaaaa
     4441 cacgctggat acgcgtacca ttcgcggact tggcttaatc tcccgccatt cattgggtat
     4501 atacggattc cacgccttga ttatccatgc gctgcgcacc cggggaattg agcttaaaaa
     4561 ttggccaata ctggatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact
     4621 ttctatgctg gtacaacgaa tcgacagaaa cagattagtg agttaagtaa aagcccgttc
     4681 acattggact gaccgggctt acgtgaatta ttcgctgtat tcttcctggg taacctgtgt
     4741 ttcttttggc gggtttattc cggcatcacg aatattcttc tgcaccgcta ctgcaccaaa
     4801 caagcttaag aagaaagcca gtccataaaa acctttttca ctgagtaata atgtcgcatt
     4861 ccacagacct accatcagta atgcaacaga gataataaag acagtcaggc aggtcatata
     4921 ataaatggaa gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc
     4981 tgcagaaaac agtcctaaca ccagtacggc aaaataatac cctttttcat ttaactgcat
     5041 ctctgcattc catagcccta acagataggt aacgatacca ccaacgagag ctatccatga
     5101 cacaatacta aaggccggtg aataggttga tattttgttg tccatcacag tattcctttc
     5161 attcctgaat gtgtaagagc tttatgttgc tcataccgag cgaaacaaac gcatttgacc
     5221 aaacaagcag acaaaaaagg cgatggggta gatcaccatt tcataagcat tgataggtgc
     5281 atttaaaaca ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg
     5341 ggtaaaaaaa ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttaccaaa
     5401 agttgctgtc attaacacgc caagaaaata gccttttcca cttagcaatg ggcatgagat
     5461 ccatagccca accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtttt
     5521 tgaggttttc ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag
     5581 acaaaactga aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa
     5641 ttaattagtg agatatatag atgtgaatta tcccccaccc ggtcaggcag gggataacgt
     5701 ttacgccatt aatggcagaa gttgctgata gaggcgacgg aacgtttcac gtcgtggctg
     5761 ataagcggca taacgctgcg catctggtag atgcgactgt tctaacggta gctgcggcaa
     5821 caactcaatg agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag
     5881 tgctggcccc acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag
     5941 catctgacgc cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt
     6001 aatgccgcag gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag
     6061 cactgctcgc gccagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt
     6121 cgcctgggga ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa accaaactgg
     6181 ctcggcactt tcatcagcct gttgagctgc agcgattaaa gctgggacat tgcacaggcc
     6241 ggttaatttc gcgacccaat ccagacacga tgctgcactc agcattacag acattaaatg
     6301 ccaacgctgc ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct tgcttaagaa
     6361 cccttcgctg acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc
     6421 atcaaccatt cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg caacaactgg
     6481 caccgtcgcc ataccccacg ctttcgcaac ttcaggtaac aaagcaccag taatttcgct
     6541 gccttcgtat aatgcgggca tctggtcacg agataagtcg caagcctgca gcatgacgtc
     6601 actccagtca cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct
     6661 ggcaaactcc cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat
     6721 ttgacggaat atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc
     6781 gggcatcatc aggttgccgg taatcactcg tgattgcgga actctcgctt ccagcaaagt
     6841 gcactcttgc gcacagcgcc cgtcgttcca caaaatggca gggcgcaata cccgttgttg
     6901 agcatccagt aaggttgctc catgcatctg gccggcaata cccaatgctt taacgtcctg
     6961 cagagaatgc tgatcgccca gagctttcat tgcgcgatca gttgcctgcc accactgttc
     7021 cgggtcttgt tccgaccaga gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga
     7081 agcaaccacc tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc
     7141 tatcccgata tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc
     7201 gggccaacgg actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt
     7261 tccagttgct cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat
     7321 ttggctaaat ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc
     7381 cagccggaat aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct
     7441 gcaattttca gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca
     7501 tatttatcag tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa
     7561 ccgcctgctt tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac
     7621 tggtcggtgt cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg
     7681 ccaagcgcaa tggcggtggc tatttcatga tggaaagagt gacctgccag cgtcgcgtgg
     7741 ttagcttcaa tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag
     7801 accgtcgcgg catcgtaatc atattgatgt ttagtcggtt cttgcggttt cggttcgata
     7861 agcaacgtgc cctggaagcc gattttatgt ttatgctcaa ccaccatctg cataaagcgg
     7921 cccagttgtt cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga
     7981 ccaccccaca ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca
     8041 acttgcgttg ccgcccagct gaagacttca ggatctgggt tcgtcgccgc acccgcgcca
     8101 tagcgagggt ttgtaaagca gttagcggtt ccccacagca gcttcacgcc gctctcttct
     8161 tgcttggctg ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac
     8221 gcgccctcag gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg
     8281 aaaaactcaa atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc
     8341 tgccacggac gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaaggtg
     8401 tgccagtagc aggcggcaaa acgcaagtgc tcttccatac gcttgcccaa caccagttcg
     8461 tcgggattgt agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg
     8521 cgatcgagct ggtcaaaata ggcttgcata ttgaactcca taatcaggta ataccgcggg
     8581 tgatggatga tgtcgtaata ttgggcactc cctttcagtt gctcaattat gttatttcac
     8641 actgctattg agataattca caagtgtgcg ctcgctcgca aaataaaatg gaatgatgaa
     8701 actgggtaat tcctcgaaga gaaaaatgca ataagtacaa ttgcgcaaca aaagtaagat
     8761 ctcggtcata aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt
     8821 gttttcccct gtttagtcgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt
     8881 aaagctgtcc tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac
     8941 cctttgcacc tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg
     9001 tatggcgatt gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa
     9061 aaaggcagaa tctctcggcg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac
     9121 acaaatgtcg cagattgaaa acatgataaa ccggggtgtc gatgttcttg tcattattcc
     9181 gtataacggt caggtattaa gtaacgttgt aaaagaagcc aaacaagaag gtattaaagt
     9241 attagcttac gatcgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa
     9301 cgaaaaagtc ggtgaactgc aggcaaaagc cctggtcgat attgttccgc aaggtaatta
     9361 cttcctgatg ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat
     9421 gaaagtgtta aaaccttacg ttgattccgg aaaaattaaa gtcgttggtg accaatgggt
     9481 tgatggctgg ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa
     9541 taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc
     9601 attaagcgcg caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc
     9661 aggtattaaa cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacgtt
     9721 gttggcaaat actgccgcag aaattgccgt tgagttgggc aatggtcagg aaccaaaagc
     9781 agataccaca ctgaataatg gcctgaaaga tgtgccctcc cgcctcctga caccgatcga
     9841 tgtgaataaa aacaacatca aagacacggt agttaaagac ggattccaca aagagagcga
     9901 gctgtaagcg ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa
     9961 tgaattggct taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa
    10021 accttcggca gtgtgaaggc gattgataac gtctgcttgc ggttgaatgc tggcgaaatc
    10081 gtctcacttt gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt
    10141 atttatcccc atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg
    10201 agtcacatcc gcgataccga acgcaaaggt atcgccatca ttcatcagga attggccctg
    10261 gtgaaagaat tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc
    10321 attatggatt atgacctgat gacgctacgc tgtcagaagc tgctcgcaca ggtcagttta
    10381 tccatttcac ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa
    10441 attgccaagg cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca
    10501 ttaactgagc aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt
    10561 atcgcctgta tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt
    10621 tgcgttattc gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac
    10681 gatattatca ccatgatggt cgggcgagag ttaaccgcgc tttaccctaa tgaaccacat
    10741 accaccggag atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt
    10801 catattaaac gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt
    10861 gccggactcg ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc
    10921 ggacaatggg aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag
    10981 caagccatcg cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt
    11041 ccggtaatgg cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc
    11101 attagccagc ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat tcagcaactc
    11161 aaagttaaaa cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa
    11221 aaagcgatcc tcgctcgctg tctgttactt aacccgcgca tcctcattct tgatgaacct
    11281 accaggggta tcgatattgg cgcgaaatac gagatctaca aattaattaa ccaactcgtc
    11341 cagcagggta ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc
    11401 gatcgggtac tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg
    11461 actcaggagc aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc
    11521 gtctgaagtg aaattggccg taccgacatc cggtggcttt tccgggctga aatcactgaa
    11581 tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac
    11641 caccgatggt gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat
    11701 taccggcatc ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc
    11761 cgtcggctca atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg
    11821 ctggcctttg ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg
    11881 gaacggatgg tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat
    11941 gttggcattt cgcggcatac tcattggcat caccaacggc acgactgttt cccccaccag
    12001 cgccgcgatg tcacaaattg ggcaaagcta tctacctgcc agcactggct tcatcattgg
    12061 cgcgcttggc ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc
    12121 tttgggtttg cagtctccgg cctctaccgc agtagtcggt cgccaggctt taaccgctat
    12181 catcgtatta ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct
    12241 gctgctgacg ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg
    12301 acgcatttat gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga
    12361 acgcaccaaa cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat
    12421 ccttagttct cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc
    12481 aattgcagca tgcgtgattg gcggcaccag cctggctggc ggagtgggaa gcgttgccgg
    12541 agcagtaatg ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt
    12601 accgaccttc tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga
    12661 ctccgcaacc aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat
    12721 taccggtttt gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac
    12781 cgcatcacat tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta
    12841 ggggaatatt tacaggcgtc acaatcggaa tgggatattt tcattgaaga agatttccgc
    12901 gcccgcattg ataaaatcaa ggactggtta ggagatggcg tcattgccga cttcgacgac
    12961 aaacagatcg agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg
    13021 tatcaccttg cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg
    13081 gttgaaagcg catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt
    13141 cttccggaat caagcggcaa acgttgggcc actgagcgcg aatatgcatt tcgtcagctt
    13201 gtcgctgaag aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac
    13261 tggcaacacg cgcaaaatcg gctggcagac tggctacaaa cgctgccacc gcaaaccggg
    13321 attattgccg ttactgacgc ccgagcgcgg catattctgc aagtatgtga acatctacat
    13381 attcccgtac cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat
    13441 ctgtcgcgtg tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg
    13501 gcaaaactgt tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc
    13561 ccaccagttc gcgtcattga acggcgctca acagattatc gctcgctgac cgatcccgcc
    13621 gttattcagg ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag
    13681 gtactggatg cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg
    13741 ggtgaaacca tccatgccat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg
    13801 atttcaacca ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa
    13861 tatttctact ctgtttttaa aaaagcatat gacacaacgc caaaagagta tcgcgatgta
    13921 aatagcgagg tcatgttgta gcgatgatga gaattgtcgg cgtcacatca gggaactcag
    13981 cacagccacc tggtgcgagc aaaagcctta tccgaccgac aaatcagata aggctcaagg
    14041 aaatgcaatt acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg
    14101 tagttgttat aactcttccc tttggtcgag tagcccttca gcttgtgaat catcgctgtg
    14161 gcagtcactt cctgatccgc tttacgcagc tgcgcacgcg atttacggaa cgaagagtaa
    14221 gccgggtgcg tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac
    14281 tgtgagtacc ctttcacttt acctggcgca ttggtacaac gtcctttcat gcatttcatg
    14341 ccgaacaggt tgttgttgtt gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca
    14401 gccatcgtcg ccaccatact ggtggggata atgtctacgc gttcaagcaa ggtattccac
    14461 gggatttttc gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga
    14521 cgcgcacgct cagcaggtga ccattggccc tgatactgtt ttgaaatcag ccagttacgt
    14581 tctgcagcaa tggctgcatt ttggctggta atgtaaggca ttacggtccg gagaaacgcc
    14641 tttttccttg gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca
    14701 ctattgcgag aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg
    14761 gacttttgac tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga
    14821 gtgagtaaca taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct
    14881 cctggtcgga tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct
    14941 caaatcatat caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta
    15001 tgtcacgtgt taacgattaa gattggcgct aaattgcaga aaatgtgggg tttatcgcaa
    15061 aattcagccg ttttttgcgc gagatcgctc acccttgctt ctcatcctgt ggacttaccg
    15121 ctcagggatg agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag
    15181 gactcatcca ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt
    15241 gccgccagct ggacttctcc ggggttccct gcctttagcg aacagggaac gggaacattt
    15301 gtcagccacg cgcagttgcc caaaggtacg cgtccactca cgctaaattt tgaccaacag
    15361 tgctggcagc ctgcagatgc gataaaactc aatcagatgc tttccctgca accttgtagc
    15421 aacacgccgc ctcaatggcg attgttcaga gacggcaaat atacgctgca aatagacacc
    15481 cgctccggta cgccaacatt gatgatttcc atccagaacg ccgccgaacc ggtagcaaac
    15541 ctggtccgtg aatgcccaaa atgggatgga ttaccgctca cgctggatgt cagcgccact
    15601 ttcccggaag gagccgccgt acgggattat tacagccagc aaattgcgat agtgaagaac
    15661 ggtcaaataa cgttacaacc cgctgctacc agcaacggtt tactcctgct ggaacgggca
    15721 gaaactgacg cccctgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca
    15781 gatcgtttcg aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt
    15841 atggcggaaa ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat
    15901 tacctccagc agttgggcgt taatgcttta tggataagcg ccccatttga gcaaattcac
    15961 ggctgggtcg gcggcggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac
    16021 acacaggact ggacgaatct tgatgccaat atgggcaacg aagccgatct acggacgctg
    16081 gttgatagcg cacatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc
    16141 ggctatgcca cgctggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt
    16201 gacgaggtga aaaaaacgct gggtgaacgc tggagcgact ggaaacctgc cgccgggcaa
    16261 acctggcata gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg
    16321 tggggaaaaa actggatcag aacggatatc ggcgattacg acaatcctgg attcgacgat
    16381 ctcactatgt cgctggcctt tttgccggat atcaaaaccg aatcaactac cgcttctggt
    16441 ctgccggtgt tctataaaaa caaaacggat actcacgcta aagtcatcga aggctttaca
    16501 cctcgcgatt acttaaccca ctggttaagt cagtgggtcc gcgactatgg gattgatggt
    16561 tttcgggtcg ataccgccaa acatgttgag ttgcccgctt ggcagcaact gaaaaccgaa
    16621 gccagcgccg cgcttcgcga atggaaaaaa gctaaccccg acaaagcatt agatgacaaa
    16681 cctttctgga tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc
    16741 cacggcttcg atgcgatgat caatttcgat tatcaggagc aggcggcgaa agcagtcgat
    16801 tgtctggcgc agatggatac gacctggcag caaatggcgg agaaattgca gggtttcaac
    16861 gtgttgagct acctctcgtc gcatgatacc cgtctgttcc gtgaaggggg cgacaaagca
    16921 gcagagttat tactattagc gccaggcgcg gtacaaatct tttatggcga tgaatcctcg
    16981 cgtccgttcg gtcctacagg ttctgatccg ctgcaaggta cacgttcgga tatgaactgg
    17041 caggatgtta gcggtaaatc tgccgccaac gtcgcgcact ggcagaaaat cagccagttc
    17101 cgcgcccgcc atcccgcaat tggcgcgggc aaacaaacga cactttcgct gaagcagggc
    17161 tacggctttg ttcgtgagca tggcgacgat aaagtgctgg tcatctgggc tgggcaacag
    17221 tgacttttcc ggcttcccgt tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt
    17281 cagccctaat cagcgttgca ggataaagca ccgctcactc tccaacagac cgatttgcac
    17341 cccagcaaat gtagcgttat tgttaccttc cttgctacag agttcgacag atatcccgct
    17401 atgacattct ccctttttgg tgacaaattt acccgccact ccggcattac gctgctgatg
    17461 gaagatctga acgacggttt acgcacgcct ggcgcgatta tgctcggcgg cggtaatccg
    17521 gcgcagatcc cggaaatgca ggactacttc cagacgctac tgaccgacat gctggaaagt
    17581 ggcaaagcga ctgatgcact gtgcaattac gacggtccac aggggaaaac ggagctactc
    17641 acactgcttg ccggaatgct gcgcgagaag ttgggttggg atatcgaagc acagaatatt
    17701 gcactaacaa acggcagcca gagcgcgttt ttctacttat ttaatctgtt tgccggacgc
    17761 cgtgccgatg gtcgggtcaa aaaagtgctg ttcccgctgg caccggaata cattggctac
    17821 gctgacgccg gactggaaga agatctgttt gtctctgcgc gtccgaatat tgaactgctg
    17881 ccggaaggcc agtttaaata ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc
    17941 gggatgattt gcgtctcccg gccgacgaat ccaacaggca atgtgattac tgacgaagag
    18001 ttgctgaagc ttgacgcgct ggcgaatcaa cacggcattc cgctggtgat tgataacgct
    18061 tatggcgtcc cgttcccggg tatcatcttc agtgaagcgc gcccgctatg gaatccgaat
    18121 atcgtgctgt gcatgagtct ttccaagctg gggctgcctg gctcccgctg cggcattatc
    18181 atcgccaatg aaaaaatcat caccgccatc accaatatga acggcattat cagcctggca
    18241 cctggcggta ttggtccggc gatgatgtgt gaaatgatta agcgtaacga tctgctgcgc
    18301 ctgtctgaaa cagtcatcaa accgttttac taccagcgtg ttcaggaaac tatcgccatc
    18361 attcgccgct atttaccgga aaatcgctgc ctgattcata aaccggaagg agccattttc
    18421 ctctggctat ggtttaagga tttgcccatt acgaccgagc agctctatca gcgcctgaaa
    18481 gcacgcggcg tgctgatggt gccggggcac aacttcttcc cagggctgga taaaccgtgg
    18541 ccgcatacgc atcaatgtat gcgcatgaac tacgtaccag agccggagaa aattgaggcg
    18601 ggggtgaaga ttctggcgga agagatagaa agagcctggg ctgaaagtca ctaaaaattg
    18661 cctgatgcgc tacgcttatc aggcctacat gatttctgca atatattgaa tttggaagaa
    18721 tttgtaggcc ggataaggcg tttacgccgc atctggcata aacaaagcgc actattccgg
    18781 cgttgagaaa cgccggaaaa acgtttcaaa caggctgctg ccgtagccga tgccgctgca
    18841 ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca caggccggtc
    18901 ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg cgcgaagaaa
    18961 cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca atgcaacgtg
    19021 tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg cagacattcg
    19081 cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg tggtctttaa
    19141 tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc tgattctcct
    19201 gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc gtcgcatccg
    19261 caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg ccagagagcg
    19321 cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt gtccaaagcg
    19381 ttatgtgatc gcgcccaggt catccctgac agtaaatccc agttcattag aaatagcctg
    19441 cgcggtttca cgcagtggtt tcaggagatt tttctctccc acctgtttca gacgtgatgt
    19501 cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atatcaaaca ccggaacagc
    19561 aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc tttcacgaat
    19621 gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg ttaacggctg
    19681 gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac caaacgccat
    19741 gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat aggcacgggt
    19801 tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat cgtcttcgcg
    19861 gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat gcggagcggc
    19921 gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa atttggtagt
    19981 caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact gtaatccctg
    20041 caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat gcgccaacgg
    20101 acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa acagactctg
    20161 gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc ccatcacttc
    20221 tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc acgatctcga
    20281 cagataattt ataaccactt gatttttatg tcttttgaaa ttcatcaatc agattgcttt
    20341 gttaaaaagt gatcgatata tttgaaatca agtttcgcat attgaaattt taagccaaaa
    20401 aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac atttgagcag ttaaaagcag
    20461 cctttaatcg ggtcttaatt tcacgcggcg ttgacagcga aacggctgac gcctgtgcag
    20521 agatgttcgc ccgcaccacc gaatccggcg tttattctca cggcgttaat cgtttccctc
    20581 gtttcattca acaactggaa aacggcgata tcattcctga tgcccaaccc aaacgtataa
    20641 ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc gatcggcaac ctgacggcga
    20701 aaaagatgat ggatcgcgcc attgaactgg ctgccgatca cggtattggt ctggtggcac
    20761 ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg ctggcaggcg gcggaaaaag
    20821 gctatattgg catttgctgg accaactcca tcgccgtaat gccgccgtgg ggcgcaaaag
    20881 agtgtcgcat tggcactaac ccgctgatcg tcgccattcc ttccacgccg atcaccatgg
    20941 tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga agttaaccgc ctggcgggcc
    21001 gtcagctccc ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa gaacctggcg
    21061 ttatcgagaa gaatcgccgc attttgccga tgggctactg gaaaggttct ggcatgtcga
    21121 ttgtgctgga tatgatcgct actctccttt ccgacggcgc atcggttgcc gaagtcaccc
    21181 aggacaacag cgacgaatac ggcatttcac aaatttttat tgccattgaa gtggacaagc
    21241 ttatcgacgg tcccacccgc gatgccaagc tgcaacgtat catggattac gttactaccg
    21301 ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg ccacgaattt actaccctgc
    21361 tggccgaaaa ccgccgtaac ggtattaccg ttgatgacag cgtgtgggcc aaaatccagg
    21421 cgttataagg agataagtca tgatttttgg acatatcgcg cagcccaatc cgtgccgttt
    21481 gcccgccgcc attgaaaagg ggctcgattt tctgcgcgct accgatttca acgccctgga
    21541 gccgggcgtt gtcgaaatcg acggcaaaaa tatttatgcg caaattatcg atttaaccac
    21601 acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata ttcagtttct
    21661 ggcgtggggt gaagagaaaa tcggcattgc tattgatacg ggaaataata aagtcagcga
    21721 atcactatta gagcagcgcg atattatttt ttatcatgac agtgaacatg aatcatttat
    21781 agaaatgata ccgggcagct acgcaatttt ctttccgcag gatgttcacc gaccaggttg
    21841 tattttgcaa acagcctctg agatacgaaa aattgtggtt aaagtcgcat taacggcgct
    21901 gaattaatcg aataatcgtc tacgccagaa cgactgggta atgttattgc tctaactata
    21961 ttaataattt aggttgctgc acat
//
LOCUS       DAPHDX010000074        20748 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPHDX010000074 DAPHDX010000000
VERSION     DAPHDX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.074"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..1229
                     /locus_tag="RH058_002764"
     CDS             join(52..310,310..1229)
                     /locus_tag="RH058_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3138331.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT
                     "
     gene            complement(1564..1692)
                     /locus_tag="RH058_002765"
                     /pseudo
     CDS             complement(1564..1692)
                     /locus_tag="RH058_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1764..1946
                     /locus_tag="RH058_002766"
     CDS             1764..1946
                     /locus_tag="RH058_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDV3138332.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2047..2376)
                     /locus_tag="RH058_002767"
     CDS             complement(2047..2376)
                     /locus_tag="RH058_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV3138333.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2548..3606)
                     /locus_tag="RH058_002768"
     CDS             complement(2548..3606)
                     /locus_tag="RH058_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3138334.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3804..4277)
                     /gene="sbmC"
                     /locus_tag="RH058_002769"
     CDS             complement(3804..4277)
                     /gene="sbmC"
                     /locus_tag="RH058_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV3138335.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4396..5562)
                     /gene="dacD"
                     /locus_tag="RH058_002770"
     CDS             complement(4396..5562)
                     /gene="dacD"
                     /locus_tag="RH058_002770"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV3138336.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALPDYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVANWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5771..7198
                     /gene="sbcB"
                     /locus_tag="RH058_002771"
     CDS             5771..7198
                     /gene="sbcB"
                     /locus_tag="RH058_002771"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV3138337.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYIFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAIIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(7241..7468)
                     /locus_tag="RH058_002772"
     CDS             complement(7241..7468)
                     /locus_tag="RH058_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV3138338.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7482..8540)
                     /gene="yeeE"
                     /locus_tag="RH058_002773"
     CDS             complement(7482..8540)
                     /gene="yeeE"
                     /locus_tag="RH058_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV3138339.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHVSGLNQTLQHYSTEHNSIAETFNLSVWPLIAVL
                     LVITFWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(8719..10077)
                     /gene="plaP"
                     /locus_tag="RH058_002774"
     CDS             complement(8719..10077)
                     /gene="plaP"
                     /locus_tag="RH058_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV3138340.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(10067..10129)
                     /gene="yoeI"
                     /locus_tag="RH058_002775"
     CDS             complement(10067..10129)
                     /gene="yoeI"
                     /locus_tag="RH058_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV3138341.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(10344..11273)
                     /locus_tag="RH058_002776"
     CDS             complement(10344..11273)
                     /locus_tag="RH058_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3138342.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(11319..12143)
                     /locus_tag="RH058_002777"
     CDS             complement(11319..12143)
                     /locus_tag="RH058_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3138343.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(12226..12480)
                     /gene="yoeB"
                     /locus_tag="RH058_002778"
     CDS             complement(12226..12480)
                     /gene="yoeB"
                     /locus_tag="RH058_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV3138344.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(12477..12728)
                     /gene="yefM"
                     /locus_tag="RH058_002779"
     CDS             complement(12477..12728)
                     /gene="yefM"
                     /locus_tag="RH058_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV3138345.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            13011..13061
                     /gene="hisL"
                     /locus_tag="RH058_002780"
     CDS             13011..13061
                     /gene="hisL"
                     /locus_tag="RH058_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV3138346.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    13037..13160
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13207..14106
                     /gene="hisG"
                     /locus_tag="RH058_002781"
     CDS             13207..14106
                     /gene="hisG"
                     /locus_tag="RH058_002781"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV3138347.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            14112..15416
                     /gene="hisD"
                     /locus_tag="RH058_002782"
     CDS             14112..15416
                     /gene="hisD"
                     /locus_tag="RH058_002782"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV3138348.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLTAAERLTAHKNAVTLRVNALKEQA"
     gene            15413..16483
                     /gene="hisC"
                     /locus_tag="RH058_002783"
     CDS             15413..16483
                     /gene="hisC"
                     /locus_tag="RH058_002783"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV3138349.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            16483..17550
                     /gene="hisB"
                     /locus_tag="RH058_002784"
     CDS             16483..17550
                     /gene="hisB"
                     /locus_tag="RH058_002784"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV3138350.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSHVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            17550..18140
                     /gene="hisH"
                     /locus_tag="RH058_002785"
     CDS             17550..18140
                     /gene="hisH"
                     /locus_tag="RH058_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV3138351.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGIDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            18140..18877
                     /gene="hisA"
                     /locus_tag="RH058_002786"
     CDS             18140..18877
                     /gene="hisA"
                     /locus_tag="RH058_002786"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV3138352.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            18859..19635
                     /gene="hisF"
                     /locus_tag="RH058_002787"
     CDS             18859..19635
                     /gene="hisF"
                     /locus_tag="RH058_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV3138353.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            19629..20237
                     /locus_tag="RH058_002788"
     CDS             19629..20237
                     /locus_tag="RH058_002788"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV3138354.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(20276..20521)
                     /locus_tag="RH058_002789"
     CDS             complement(20276..20521)
                     /locus_tag="RH058_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3138355.1"
                     /translation="MISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(20598..>20748)
                     /locus_tag="RH058_002790"
     CDS             complement(20598..>20748)
                     /locus_tag="RH058_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDV3138356.1"
                     /translation="HCQLNNTSPSIRPSLYAYERYLHHVLDVIADWPVNRVGALLPWR
                     VTLPA"
BASE COUNT         5340 a         5410 c         5222 g         4776 t
ORIGIN      
        1 gccacctgat gaaaggtgat attctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gaaccttcac ccctgagttc aggctggaat gtgcacagct gattgttgat
      121 aagggctact catatcgaca ggccagtgaa gcgatgaatg tcggttcaac cacgcttgag
      181 agctgggtac gccagctcag gcgagagcgc cagggtatta cgccctctgc cacacccatt
      241 actccagacc agcaacgtat ccgcgagctg gaaaagcaag ttcgccgtct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttaatgtccg actcgctgaa cggttcacga
      361 tagccgccag actaagtgac agccacacgg ttgtcagcct gtgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcacgagtca
      481 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtcg gggctctgcg ggcgcgcgca
      541 ctctggctga aatgctgact caaaacggcg tcccgatgag ccgttaccgt gccggacgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtat aaaaatgccc
      661 gtcaggagca taccagcctg ccgaatctgc ttgagcgtca gttcgcagta ccggagccag
      721 accgggtatg gtgtggagat attacgtatc tctgggcagg aaatcgctgg tgctatctgg
      781 cggttgttat ggatcttttt gcccgcaggg ttatcggctg gagtctgtca gcgcatgccg
      841 ataccgcact gataagcagt gccctgcgga tggcctatga gacgcgtggc caaccccggg
      901 atgtcgtgtt ccatagcgac cagggaagcc agtatacagg ccttaaatat caacaacttc
      961 tctggcgttg cagaataaat caaagcgtca gtcggcgggg aaactgctgg gataacagcc
     1021 ccatggaacg cttcttccgc agtctgaaaa cagaatgggt gccaacgaat ggttacgcag
     1081 gcaaggacga ggcccggcag caaattaatg attacatatt gaactactac aacagcgtca
     1141 gacctcacca ttataacggt gggctgacgc cggaagagtc agagaacaga taccattttt
     1201 actgtaaaac cgtggccaat attacttgac cactacaccc tgcgccatct gcagcttctg
     1261 caccagaacc ggtgaatctc actcaacagg cttcatttct cctgacgcca gcctgaatac
     1321 agctggcgtt ttcatttata aacagaaagg aaaaccgata ataatggaat ggacgccccg
     1381 accatgaagc aacggtagga tattactgat aaaccttcca gtggaggtgc gttatggccg
     1441 ttttaacgat tagcattgac ctgacaaaaa atgaattcca aatccacggt ccgggtagga
     1501 acagaaaaat ctaagctcag aaaacggatt aaagtaagcg tcaacggagc accgtattga
     1561 cgcttattta ttggtgagaa ctacgttcca tggcaggagt tcgtcaacac ggttggaggg
     1621 ccattccggc agtatgctca gaatatggcg cagatacgct tccggatcga taccgttcag
     1681 acggcaggtg cctgcttttc tggcaacaag caggaatctc agcgctgaag caggaattag
     1741 aggtaaaaac gccctaccgg gccatgaatc acccggtcat tggagtagta accaaagcag
     1801 atttagccag catggaacaa atatctttgg tgaaaagttg gttacgggaa gctggagcgc
     1861 acaacgtgtt agtaaccagt gcagttaata acaatggagt tacagaactt tttgccttgc
     1921 tgcatacaga agaaggctgt tgttaatttc gattggttct cggatgagaa tgatgacggt
     1981 gatacacaga taagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa
     2041 cgggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
     2101 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc
     2161 atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tccccggctt
     2221 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
     2281 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
     2341 cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga
     2401 gtcggattcg tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag
     2461 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
     2521 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
     2581 tagcaactca agctgatgcg ccgtttccat gttcagccac acataaccat agattggtgt
     2641 ttccacaacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc
     2701 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
     2761 aaggctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa
     2821 taacacgaaa tggctggggc gcgtggccca gtatgcattg atttgcaact ccagcataca
     2881 aaccagattg cggttaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc
     2941 tttgctggcg ggcgcaatca gtccacgcat tttcacggca tcggtcagga gtttttgtag
     3001 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gatagacccg
     3061 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
     3121 ccagataccg gtaaacaaca ttgccagtaa agagccgagg atcacatcgc cgcttcgcca
     3181 taacgccgta tcaatttcac ctgtcgggga acccacaaca attgccagcg tcaccccaat
     3241 caataaacct tgatacggtt tcttgcccag cgccagccaa ccgcaaagga acatggccgc
     3301 cgcgcaccag actaacatca gcggtaacga gattaactcc agttgcagag cgataagacc
     3361 taaaatcgaa cccaacaccg taccgccaat acgctcaaag gcgcgaggga caacgttacc
     3421 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
     3481 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
     3541 atgcacaatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
     3601 acgcacgcag aaactccgta atgagattaa aagcgctcat aactcatctg tcggccagaa
     3661 ggcgcgcctc tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc
     3721 gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa aaaaaagagt ggtcatcgcg
     3781 ttaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
     3841 atatcccagt acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt
     3901 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
     3961 aaatcatcac cgactacacg agctaccgcc accgcatact gaccacctgt aatttctgtc
     4021 agaatgacgc cctcactgtt ttcgggaagc gtaaagtaac ccggcaccgt cacgacggtg
     4081 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaatagac agcaacccac
     4141 tccttcggca caatattttt gctatctacc cacatcatca actgctcaaa gcctttcttt
     4201 accgtctgtt cccacgggcc aacgagatgg aaacctgcaa cggtacgttt ctcttcctgc
     4261 ttaatctcgt agttcatgac gcctccattg atactgtttt tatatacagt atagttgcaa
     4321 aattaaaacc acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct
     4381 gctgcaaaag aaaggtcagg ccttatggtg gaaataatca ctcaggcgag aaaacatgct
     4441 gccttccccg acagattcca gggtaaccag cggccagttc gccacctgtt tatcacggtc
     4501 gtaaagttca atttccccta cccgctgatg ggcgctaatt ggcgcggtga gttctttacc
     4561 atcaagggta tatttggctt tgatatgtgg aatttcggct ttcggtagta ccatccagaa
     4621 ctcttgttct gttcccaggg cgatattttc tttatcgcca taccatatgc gttccgtacc
     4681 gacctttttc ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag
     4741 taattttctt gcctcttcct cacgaccttt tgcgctgtca gcacccatta ccactgcaat
     4801 gagacgacgc tgcccatcta cagccgaagc aatgagatta aacccagcac cagaagtatg
     4861 acccgttttc aggccgtcaa cattcatggt tttatcccac aataacccgt tacggttttg
     4921 ctgggtgata ccgttccagg tgaggctttt ctcactgtac atatgataaa actcgggctc
     4981 gccgtggatg atagcacgag aaagcacagc taaatcataa gccgagctat gctgacctgg
     5041 cgcatccagg ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata
     5101 gttgttcatc atttcaacaa actgccgttg cccaccggca atatagtcag gcagtgcaac
     5161 acaagcgtca tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg
     5221 atcgccctct ttcaaaaaca tcagtgaaga accgacaaac accggattat ctttcgccca
     5281 cgcatcgcgc cccacggtga caatatcgtc tggcgtaatg cgatgactat cgatagcgcg
     5341 atccacgaca taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc
     5401 attacccgcg gtgaggatct gaccggtggt gtaatccatc agtacccagg acccggcatg
     5461 aatctctgga ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt
     5521 aaaaacgaac aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt
     5581 ttcaaatagc tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa
     5641 aatgtgacta ccctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt
     5701 ggcgctaaag ctgattagca cggtgatatt tgatactctg gcagacagca gaaataacgg
     5761 atttaaccta atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga
     5821 aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga
     5881 tagcgaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg cggatgacta
     5941 tttgccccag cctggagcag tattaattac cggtattacc ccgcaggaag cacgggcgaa
     6001 aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaacg taccgaagac
     6061 ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta
     6121 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt
     6181 gctggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa
     6241 tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gtattgaaca
     6301 tagcaacgcc cacgatgcta tggctgatgt gtacgccact attgcgatgg caaagctggt
     6361 aaaaacgcgt cagccacgcc tgtttgatta tatctttacc catcgcaata aacacaaact
     6421 gatggcgttg attgatgttc cgcagatgaa acccctggtg cacgtttccg gaatgtttgg
     6481 ggcatggcgc ggcaacacca gctgggtggc accgctggcg tggcatcctg aaaatcgcaa
     6541 tgccataatt atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga
     6601 cacattgcgc gagcgtttat ataccgcaaa aaccgatctt ggcgataacg ccgccgttcc
     6661 ggttaagctg gtgcacatca ataaatgtcc ggtgctggcc caggcgaata cgctacgccc
     6721 ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc tgaaaattct
     6781 gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt
     6841 tacgccttca gataacgtgg atgcacagct ttataacggc tttttcagtg acgccgatcg
     6901 cgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
     6961 ttttgttgat aaacgaattg aaaaactgtt gttcaattat cgggcacgca acttcccggg
     7021 gacgctggat tatgccgagc agcaacgctg gctggagcac cgccgccagg tcttcacgcc
     7081 cgagttttta caaggttatg cagatgaatt gcagatgctg gcacaacaat atgccgataa
     7141 caaagagaaa gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtctaatt
     7201 tgaagcctcc gccgctggta cggcggaggt caatgttgag tcaggctttt tgaacggtga
     7261 tactccacgc tgcatcgcca atctgctgat aatcggtgat ggcatgccct tcttctgccg
     7321 cccactgtgg gattgcttca gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc
     7381 cgctggccat ttctgccagt gctgcttttg cttctatgag tggaaacgga cagacctgcg
     7441 tcaccacatc cagcttttta atcgccatct ttacacctta cttaatttgc cgcagcagtt
     7501 gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc aggacgcagt ccagactccg
     7561 agaatcataa atacaaggcc aatccagccc tgccaggtca tcattgcagt cataaccagt
     7621 ccattaccga tagagcaacc acctgcaata ctggcaccga agcccatcag tacaccacct
     7681 aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct
     7741 ttggctgcaa taaatgaccc cacgaagatc cctaacacca ggaaaacgcc ccagttaatg
     7801 tatttcacgt cacccgcgac cagaaattgc agaatattgg ccgttgggga agtgattcct
     7861 aacccaaaca tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg
     7921 atgagtacag cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg
     7981 gttcggcgcg gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc
     8041 acccagaaag ttatcaccag caaaacggca atcaacggcc acacagacag attaaacgtc
     8101 tcggcaatgg agttatgttc agtactgtag tgctgcaagg tttgatttaa accactgaca
     8161 tgtggagaac gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg
     8221 atcaatccct cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt
     8281 cccagcccga agatataccc acctataaca gtaccaagcc acgggaacgc cccggcttcg
     8341 taagtcagta ggcccgcctg aattaacgca aaaaccccca cgctttgtac cgaaattgca
     8401 atcagcaagg cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacct
     8461 gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg
     8521 cttaatatca ttgaaaacat aaattaatta accagatgaa tgttaatggg gaaattattc
     8581 atgactggtg gaatgcagac caataaccaa attgtctaaa ttagaacaaa tggttattaa
     8641 tgaggatgtc gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt
     8701 tattattaga aaagatgatt acgctacgtc ttcgtactgc ggaaccgggt tacggaaact
     8761 cttggtgacg caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat
     8821 tgagctttct tccaggttaa cccacagcgc accaacggtc agtgcaccac acatcggcag
     8881 gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg
     8941 cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc
     9001 tgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat
     9061 gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaacac
     9121 gccgtcacga cccattacgt acatcagacg cgcaacacct gcgtgcgccg ccataccgga
     9181 cgccagtacg gtaatggtgg agaagatcag cgcaccaacc tggaatgctt tacctgccac
     9241 gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa
     9301 gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag
     9361 gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat
     9421 gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac
     9481 atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc cttcaccttc
     9541 aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac
     9601 gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa
     9661 ggccaccagc gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata
     9721 gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca
     9781 acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtat atgcagagcc
     9841 agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat
     9901 caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa
     9961 agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa
    10021 cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt
    10081 cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt
    10141 cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc
    10201 ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt
    10261 agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag
    10321 aggcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa
    10381 acaccggcga tcgcactatt tgctaaaatt tcatctcgcc accagccgct ggcaagccct
    10441 gcggtctgtt cgttccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc
    10501 ttctcgatta acgcgccact atcgagaaaa cgttgcgcca gataacgggg taaatagccg
    10561 cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaagac ggtaatggct
    10621 tcttgttcgt cgagaagttg tgaggccgta gaagcgccgg cctgcgcagt gtcccccacc
    10681 acaatcgccc gatatcgctt aatgatacga cggtttaacg gttcttcttc cagggctaac
    10741 ggatgatgag gcgctaccgc aaagacttgt tccagatcgc ccaaccgcga gaaaccaaac
    10801 tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc
    10861 agggcatccc atgaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga
    10921 tgttgataaa aagcttcaat gagtggcgca agcagagaaa aaggaaacgt gtcgtccacg
    10981 ccaatcacca gttcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct
    11041 cgcacggtat gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat
    11101 ttagcgcggt gtccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga
    11161 acggtgtaac tcagagcgga cggcgtctta tagagtttgg ccgacgccgc cgcaaaactg
    11221 ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc
    11281 cccttgcagt gcatgcggcg tcctgctggc gtggtcgttt actccagcgg cattaccagc
    11341 ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc
    11401 ttacctttgc cgctgtccag actatttctg aactgcggag gctccagccc cagtaaacgg
    11461 gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg
    11521 tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg
    11581 actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga
    11641 tgacgccccg gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa
    11701 ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc
    11761 ggggtagttt ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa
    11821 ataattcggg gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa
    11881 tagaactcat cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca
    11941 tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga
    12001 tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg
    12061 gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac
    12121 ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta
    12181 cgctagcgta tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg
    12241 acatgctgca atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt
    12301 aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc
    12361 ttcaaatggc gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt
    12421 atctgtttcc tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac
    12481 tcaataatgt ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
    12541 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct
    12601 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga
    12661 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
    12721 gtacgcatgt caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac
    12781 tatttattaa ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc
    12841 tatttttggt gccgtcagct tagaggacag tcctcttagc gccctccttt ccccgctcat
    12901 tcattaaaca aatccattgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
    12961 gtggtttagg ttaaaagaca tcagttgaat aaacattcaa agagactttt atgacacgcg
    13021 ttcaatttaa acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc
    13081 tggaagacat tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca
    13141 ccttccgggg gcttttttat tgcgcgattg ataacggttc agacaggttt aaagaggaat
    13201 aagaaaatga cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt
    13261 gatgactcac gtgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc
    13321 ctgatcgcga tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt
    13381 cccggtctgg taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa
    13441 gaagagctgc ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt
    13501 ctggatttcg gcggctgccg tctttcgctg gcaacgccgg ttgatgaagc ctgggatggc
    13561 ccgctctcct taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat
    13621 ctcgaccaga aaggcatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc
    13681 ccgcgtgccg gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa
    13741 gcaaacggcc tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc
    13801 gatggcgaaa tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag
    13861 ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg
    13921 gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt
    13981 gaccaacaac gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg
    14041 gaaaaactga aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg
    14101 gagtgatcgc catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac
    14161 aacgccagct gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta
    14221 acgatattct cgataacgtg aaaacgcgtg gcgatgaagc cctgcgtgaa tacagcgcga
    14281 agtttgataa aaccacggtt accgcgctga aggtgtctgc tgaggaaatc gccgccgcca
    14341 gcgaacgcct gagcgacgag ttaaaacagg cgatggcggt ggcagtaaag aatattgaaa
    14401 ccttccacac tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtacgtt
    14461 gccagcaggt cacgcgcccg gtggcttcag tgggtttgta tattcctggc ggctccgccc
    14521 cgctcttctc aacggtatta atgctggcaa ctccggcgcg tattgcaggt tgcaataaag
    14581 tggtgctgtg ctcaccgccg ccgattgccg atgagatcct ttacgcggcg cagctgtgcg
    14641 gcgtgcagga cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta
    14701 cggaatctgt gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag
    14761 caaaacgcca ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggtccgt
    14821 cggaagtgct ggtgattgct gacagcggcg ctacgccgga tttcgtggct tctgatttgc
    14881 tttctcaggc tgaacacggc ccggactcac aagtgatttt actgaccccc gacgccgata
    14941 tggcgcgtcg cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa
    15001 ccgcccgcca ggcactgagc gccagccgcc tgatcgtgac taaagattta gcgcagtgcg
    15061 tagagatttc taatcagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg
    15121 agctggtcga tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat
    15181 cggcaggcga ctatgcctcc ggtaccaacc acgtactacc gacttacggt tataccgcga
    15241 cgtgttccag cctcggcctg gctgatttcc agaagcgtat gaccgtgcag gagctgtcga
    15301 aagaaggttt ctccgcgctg gcttcgacca ttgaaacact gaccgccgcc gagcgcctga
    15361 ctgcccacaa aaatgccgtt actttgcgtg ttaacgccct taaggagcaa gcatgaacac
    15421 cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac ctgacgccgt atcagtcggc
    15481 gcgtcgtctg ggcggtaacg gcgacgtctg gctgaacgcc aacgaatacc ccacagccgt
    15541 ggagtttcag ctcactcaac aaacgctcaa ccgctacccg gaatgccagc cgaaagcggt
    15601 gattgaaaat tacgcgcaat atgcaggcgt aaaaccggag caggtgctgg tcagccgtgg
    15661 cgcggacgaa ggtattgaac tgctgattcg cgctttttgc gaaccgggta aagacgccat
    15721 cctctactgc ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggcgtcga
    15781 gtgccgcaca gtgccgacgc tggacaactg gcaactggac ttacagggca tttccgacaa
    15841 gctggacggc gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat
    15901 caatccgcag gattttcgca ccctgctgga gttaacccgc ggtaaggcga ttgtggttgc
    15961 cgatgaagct tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata
    16021 tccgcacctg gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg
    16081 cggatttacg ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcgcccccta
    16141 cccgctctcg acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt
    16201 cgccatgcgc gaacgggtgg cgcaaattat tgcagaacgc gaatatctga ttgccgcatt
    16261 gaaagaaatc tcctgcgtgg agcaggtttt tgactctgaa accaactaca ttctggcgcg
    16321 ctttaaagcc tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga
    16381 tcagaataaa caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga
    16441 aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt
    16501 tttatcgatc gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt
    16561 tttgacaaac tcgcctttga accgggcgtg atccctcagc tgctgaagct gcaaaaagcg
    16621 ggctacaaac tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcag
    16681 gcggattttg atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag
    16741 tttgatgaag tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg
    16801 aaagtaaaac tggtggagcg ttatctggct gagcaagcga tggatcgcgc taacagtcat
    16861 gtgattggcg atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta
    16921 cgctacgacc gcgaagccct gaactggccg atgattggtg agcaactcac taaacgagac
    16981 cgttacgccc atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg
    17041 gatcgcgaag gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat
    17101 cagatcgcca cccacggcgg tttccggatg gaaatcaacg tcaaaggcga cctctatatc
    17161 gacgatcacc acaccgtcga agataccggc ctggcgctgg gcgaagcatt aaaaattgct
    17221 cttggcgaca aacgcggtat ttgccgcttt ggttttgtac tgccgatgga cgaatgcctt
    17281 gcccgctgcg cgctggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc
    17341 taccagcgcg tgggcgatct cagcaccgaa atgatcgagc acttcttccg ttcgctctca
    17401 tacaccatgg gcgtgacgct acacctgaaa accaaaggta aaaacgatca ccaccgtgtt
    17461 gagagtctct ttaaagcctt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgac
    17521 accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg
    17581 ccaacctgaa ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc
    17641 gtgacccgga cgttgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac
    17701 aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc
    17761 aaccggtgct gggcatctgc ttagggatgc aacttctggg gcggcgcagc gaagagagca
    17821 acggcatcga cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc
    17881 tgccactgcc gcatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc
    17941 aggggattga agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc
    18001 cgtggaccat cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata
    18061 acttctacgg cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga
    18121 aaaacttcct ggagatgtaa tgattattcc ggcattagat ttaatcgacg gcaccgtggt
    18181 gcgtctccat cagggcgatt acggcaaaca acgcgattac ggtaacgacc cactgccacg
    18241 cttacaggat tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc
    18301 aaaagatccg gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt
    18361 cccggtgcag gttggcggcg gtgtgcgtag tgaagaagac gtggcggcgt tactggaagc
    18421 gggcgttgcg cgcgtggtgg ttggctccac cgcggtgaaa tcaccagaaa tggtgaaagg
    18481 ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga
    18541 gcaaggtaac aagcaggtgg cggtgagcgg ctggcaagag aactcgggcg tttcactgga
    18601 acaactggtg gaaacctatc tgcccgtcgg cctgaaacat gtgctatgta ccgatatctc
    18661 gcgcgacggc acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata
    18721 cccgcaggtg gcatttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct
    18781 gcgaggtact ggcgtgcgcg gcgtaatagt tggtcgggca ttactggaag gtaaattcac
    18841 cgtgaaggag gctatcgcat gctggcaaaa cgcataattc catgtctcga cgttcgtgat
    18901 ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgccg
    18961 ctggcaaaac gttacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct
    19021 tccagcgatg gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac
    19081 attccgttct gtgtagcggg tgggattaag tctctggaag atgccgcgaa aattctttcc
    19141 tttggcgcgg ataaaatttc catcaactcg cctgcactgg cggacccgac gttaattact
    19201 cgcctggcgg atcgctttgg cgtgcagtgt attgtggtcg gtattgatac ctggtacgac
    19261 gccgaaaccg gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc
    19321 actcagtggg aaacgctcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaaatc
    19381 gtcctcaata tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa
    19441 aaagtacgtg aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa
    19501 cacttcctcg aagctttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc
    19561 cacaaacaaa taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag
    19621 atcaggatat gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac
    19681 tgatgccggt gattgtacaa cacgcggtat ctggcgaagt gttaatgctg ggctatatga
    19741 acccggaagc cttagacaaa accatcgaaa gcggcaaagt caccttcttc tcgcgcacta
    19801 aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt cctcaacgta gtgagtattg
    19861 cgccggactg cgacaacgac actttattag tgctggcgaa tcccatcggc ccgacctgcc
    19921 acaaaggcac cagcagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac
    19981 tggaacaact gctcgccgag cgcaaatctg ccgacccgga aacctcctat accgccaaac
    20041 tgtatgccag cggcaccaag cgcattgcgc agaaagtagg cgaagaaggc gtggaaactg
    20101 cgctggcagc aacggtacat gaccgctttg agctgaccaa cgaggcatct gacttgatgt
    20161 atcacctgct ggtgttgttg caggatcagg ggctggatct aggagaagtg attgataatc
    20221 tgaagaatcg gcattaacgc gttggccccc gcagcaacgg cgggggtagc atctctcagg
    20281 atttgctttc cagcaatgta gtgtagagat tgacataggc gtcaatcata tggtcggcag
    20341 taaacatctc ttcaaaacga cgacgcgagt tttcgccata gcggttggag gtttcctcgt
    20401 tattccagag ctcattcatc gcctctacca gcgcctggct atcattcggc ggaatcacgc
    20461 aaccactcac tttgtcctgg ttaatgaaag aggtacctgt accaatctcg caagagatca
    20521 gtggccttgc gaagcgtgcg ccttccaata acgtaagtaa gcgtgcagtg agaaccgtat
    20581 tgacgaagtg aagctggtca ggcaggcaga gtaacccgcc acgggagcag agcacccacc
    20641 cggttcacag gccagtcggc gatgacatca agtacatgat gcagatagcg ttcgtaagcg
    20701 tacagcgagg gccgtattga cggggatgtg ttattcagct ggcagtgc
//
LOCUS       DAPHDX010000075        20695 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPHDX010000075 DAPHDX010000000
VERSION     DAPHDX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.075"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(8..1657)
                     /gene="actP"
                     /locus_tag="RH058_002791"
     CDS             complement(8..1657)
                     /gene="actP"
                     /locus_tag="RH058_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV3138357.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1654..1968)
                     /locus_tag="RH058_002792"
     CDS             complement(1654..1968)
                     /locus_tag="RH058_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV3138358.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2168..4126)
                     /gene="acs"
                     /locus_tag="RH058_002793"
     CDS             complement(2168..4126)
                     /gene="acs"
                     /locus_tag="RH058_002793"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV3138359.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4518..5954
                     /gene="nrfA"
                     /locus_tag="RH058_002794"
     CDS             4518..5954
                     /gene="nrfA"
                     /locus_tag="RH058_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV3138360.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5999..6565
                     /gene="nrfB"
                     /locus_tag="RH058_002795"
     CDS             5999..6565
                     /gene="nrfB"
                     /locus_tag="RH058_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV3138361.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6562..7233
                     /gene="nrfC"
                     /locus_tag="RH058_002796"
     CDS             6562..7233
                     /gene="nrfC"
                     /locus_tag="RH058_002796"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV3138362.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7230..8186
                     /gene="nrfD"
                     /locus_tag="RH058_002797"
     CDS             7230..8186
                     /gene="nrfD"
                     /locus_tag="RH058_002797"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV3138363.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8206..9924
                     /locus_tag="RH058_002798"
     CDS             8206..9924
                     /locus_tag="RH058_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV3138364.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9917..10300
                     /gene="nrfF"
                     /locus_tag="RH058_002799"
     CDS             9917..10300
                     /gene="nrfF"
                     /locus_tag="RH058_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV3138365.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10297..10893
                     /gene="nrfG"
                     /locus_tag="RH058_002800"
     CDS             10297..10893
                     /gene="nrfG"
                     /locus_tag="RH058_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV3138366.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTALTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11235..12548
                     /gene="gltP"
                     /locus_tag="RH058_002801"
     CDS             11235..12548
                     /gene="gltP"
                     /locus_tag="RH058_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV3138367.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12626..13315)
                     /locus_tag="RH058_002802"
     CDS             complement(12626..13315)
                     /locus_tag="RH058_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3138368.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13409..15556)
                     /gene="fdhF"
                     /locus_tag="RH058_002803"
     CDS             complement(13409..15556)
                     /gene="fdhF"
                     /locus_tag="RH058_002803"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15137..15139),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV3138369.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15754..17220)
                     /gene="mdtP"
                     /locus_tag="RH058_002804"
     CDS             complement(15754..17220)
                     /gene="mdtP"
                     /locus_tag="RH058_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV3138370.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17217..19268)
                     /gene="mdtO"
                     /locus_tag="RH058_002805"
     CDS             complement(17217..19268)
                     /gene="mdtO"
                     /locus_tag="RH058_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV3138371.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19268..20299)
                     /gene="mdtN"
                     /locus_tag="RH058_002806"
     CDS             complement(19268..20299)
                     /gene="mdtN"
                     /locus_tag="RH058_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV3138372.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSIGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20318..20593)
                     /locus_tag="RH058_002807"
     CDS             complement(20318..20593)
                     /locus_tag="RH058_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138373.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4659 a         5494 c         5669 g         4873 t
ORIGIN      
        1 ggagagatta atgtgcgcgg ccttgctcaa cgccaaagcc ggtctgggag cggataaact
       61 gtgcacggaa cagttcacgc tcgcgcgcac cttccgctga gttatcggtt gccgagaaga
      121 accagatgcc gaggaatgcc acgctgatag agaacagcgc cgggtattca tacgggaaga
      181 tggctttttc gtgaccaagg atctgtaccc aaatcgtcgg gccgaggatc atcagtacca
      241 ctgcggtaat cagccccagc cagccaccca tcatcgcgcc acgagtggtc agtttcgacc
      301 agtacataga aagcagaatg atcgggaagt tacagctcgc tgcgatggca aacgccaggc
      361 ctaccatgaa ggcgatgttc tgattctcaa acagcacgcc gaggataatg gcaatcacac
      421 ctaaaatcag cacggtgatt ttcgataccc gcagttcttc acgttcggtc gcgccttttt
      481 tgaagacgtt agcgtacagg tcatgcgaaa ccgctgatgc gcccgccagc gtcagaccag
      541 caaccaccgc aagaatggtg gcaaaagcaa ccgctgaaat aaaaccgagg aacaggttgc
      601 cgcccactgc attcgccagg tgaacggccg ccatgttgtt accaccaatc agatggcccg
      661 ccgcgtcttt atattccgga ttcgcaccaa ccagcatgat cgcgccgaag ccgataataa
      721 aggtcagaat atagaagtag cccataaatc cggtggcgta gaacacgctc ttacgtgctt
      781 cgcgggcatc gctgactgta aagaagcgca tcagaatgtg cggcaagccc gccgtaccaa
      841 acatcagtcc cagccccaga gagagcgcgg agatcggatc tttcaccagt ccgcccggtt
      901 tcatgatgtc gacacctttc gggtgtaccg ccatcgcttc gctgaacaga ttgttgaagc
      961 taaagccgac gtgtttcatc accataaagg ccataaagct ggcaccgaac agcaacagaa
     1021 cggctttaat aatctgtacc caagtggtcg ccagcatgcc gccgaacagg acgtacatca
     1081 tcatcagcac gccgaccagc accaccgcaa tgtgatagtt aaggccaaac agcagctcga
     1141 tcagtttacc tgcacccacc atctgggcga taaggtaaag cgccaccacc accagagaac
     1201 cacaggccga aagaatacga atcggccctt gtttcagacg gtaagaggcc acatcggcaa
     1261 aggtgtagcg ccccaggtta cgcagacgtt cggcaatcag gaacagaatg atcggccagc
     1321 ccaccaggaa gcccagcgag taaattaagc cgtcatagcc ggaggtaaac accagcgcgg
     1381 agatccccaa aaatgaggcg gcggacatat agtcaccggc aatcgccagc ccgttctgga
     1441 agccagtgat attgccgcct gcggtgtagt agtcgctacg agaacgtacg cgttttgatg
     1501 cccagtaggt aatgccgagc gtaaacacga cgaaaatcag gaacataata atcgcctgcc
     1561 agttcgttgg ctggcgctct acggccccgc taatagcatc cgcggcgtta gctgcgaaag
     1621 ggagtgtggc ggcaagcgcc gtcagaactc ttttcatgat gcttgtacct catgcaggac
     1681 ttcgttatta agacggtcga attcgccgtt cgcccgccag atgtagatac cggtgagaac
     1741 aaaggagatc acaatcactc caacaccaat cggaataccg cgtgtgacgc tggtgttcgg
     1801 attcagcggc gtgcccagcc agccgggcgc gaaggcgatc agtaaaataa agccgatata
     1861 aactgccagc ataataatcg acaggatggt ggcaaaccgt tgccgttttt cgactaactc
     1921 cctgaaatgc gcattgtctt ctatccgctg ataaatagtg ccattcatca cagattctcc
     1981 agaggtaatg tagggattgt tttaattccc gctcccttat gggagaaggt taacgctcgg
     2041 gtaacccttg ccgaatgtag gccggataag gcgtttacgc cgcatccggc aatcaatgcc
     2101 tgatgcgacg ctgtcgcgtc ttatcaggcc tacaaaccgt taccgactcg catccggcaa
     2161 ttgtgggtta cgatggcatc gcgatagcct gcttctcttc aagcagcttc tcgactacgc
     2221 caggatcggc aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc gcaattttgc
     2281 gcagaatacg gcgcataatt ttgccggagc gggttttagg cagggagtcg gtccagtgca
     2341 gcacgtctgg cgtcgccagc gggccaatct ctttacgcac ccagttgcgg acttctgcgt
     2401 acagttctgg tgacggttcc tccccgtgat taagcgtgac gtaggcgtag atcgcctgac
     2461 ctttaatatt gtgcggaata cctactacgg cggcttcggc aatcttcgga tgcgccacca
     2521 gcgccgactc aatctctgcc gtccccagac ggtgaccgga gacgttcagc acgtcgtcca
     2581 cacgcccggt gatccagtaa tagccatctt catcgcgacg cgcgccgtcg ccgctgaaat
     2641 acatattttt gaaggtggag aagtaggtct gttcaaaacg ttcgtgatcg ccaaacagcg
     2701 tacgcgcctg acccggccag gagtcggtga tcaccaggct gccttcggta gcgccttcca
     2761 gcgggttacc ttcgttatcg accagcgccg gttgcacgcc gaagaacgga cgtgttgccg
     2821 aaccggcttt cagctcggta gcgccaggca gcggcgtgat catgaaaccg ccagtttcgg
     2881 tctgccacca ggtatcgacc accggacatt tctcgttgcc gatttttttc cagtaccact
     2941 cccacgcttc cgggttaatt ggctcgccca cggaaccgag aatgcgcagc gacgaacggt
     3001 cggtgccttc gatcgcttta tcgccttccg ccatcagcgc gcggatcgcc gtgggtgcgg
     3061 tatagagaat attgacctga tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc
     3121 agttgggtac gccttcaaac atcagcgtgg tcgcaccgca ggccagcggg ccgtacagca
     3181 agtaactgtg tccggtcacc cagcccacat cggcggtgca ccagtagata tcacccggat
     3241 gataatcaaa gacatattta aaggtcagcg ccgcgtacac cagataaccg ccggtagtat
     3301 gcagcacacc ttttggctta ccggtagaac cggaggtgta gagaataaac agcggatctt
     3361 cggcgttcat ctcttccgcc tggtgctgat cgctcgcttg ctcaaccagg tcgtgccacc
     3421 acaggtcgcg cccttcctgc cagtcaattt tcccgccagt acgcttcagt accaccacat
     3481 gctctacgct ggtgacgttc gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa
     3541 tactgcgccc ggcacgcaca ccttcgtcgg aagtgatcac cagtcgtgag ttggaatcaa
     3601 taatgcgccc ggcaacggct tccggcgaga agccgccgaa aatcaccgaa tgcaccgcgc
     3661 caatgcgggc gcaggccagc atcgcaaccg cggcttccgg caccatcggc atataaatcg
     3721 ccaccacatc acctttttta atgcccagct cgagcagggt attggcgaag cggcagacgt
     3781 cgcggtgcag ctctttatag ctgatatgtt tgctctggct ggcgtcgtcg ccttcccaga
     3841 tgatggcggt gcgatcgccg ttttcttgca gatggcggtc aaggcagttt gccgccagat
     3901 tcagcgtgcc gtcctcgtac catttaatgg acacattacc gggggcaaag gaggtatttt
     3961 tcaccttctg gtaaggtttg atccagtcga gaatttttcc ctgttcgccc cagaaggtat
     4021 caggtacgtt aatagattgt tgatacatcg cctcgtactg ctgagggttt atcaggcaac
     4081 ggtctgcgat gttggcagga atggtgtgtt tgtgaatttg gctcatgctt ttgttctcct
     4141 tgtaggatgt taataatatg tggcataagc gttaaatgta ggggtattgg cagttttgtt
     4201 tagtatttgg gcgacagatc acgcaaaagt agaattgtgc aaataaacgg cagggtaatt
     4261 tttgaaggtc agcaacaaaa gttgattaat tctttgagga acatgcagtt atgcatgctg
     4321 ttgaaaaaga ggaagatact gactaactct aaagtggtat tttacatgca cttacaattg
     4381 attaaagaca acattcacag tgtggttatt tgttacacat aggggcgagc aatgtcatga
     4441 cagtgtaggt gcggttactg tcgtgaaaac aataaaaacc gccattgcaa caatggcgca
     4501 attcggatga agcccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat
     4561 tgattccttt tttctttttc acttctgttc acgctgaaca aacggcagct cccgcaaaac
     4621 ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca gcatcccgat caatatctct
     4681 cctggaaagc cacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc
     4741 tggtgatcct gtgggcgggg tatcccttct cgcgcgatta caacaagccg cgtggacatg
     4801 cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag
     4861 atggtccgct accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc
     4921 agaaagacgg cgaagatggc tacttccacg gtaagtgggc gcgcggcggc ccggaaatcg
     4981 tcaacaactt aggttgtgcc gactgccata acaccgcctc accagagttc gccaaaggca
     5041 aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac
     5101 cttttgagaa agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg
     5161 agtattactt cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag
     5221 tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt
     5281 cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc
     5341 acggtaaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag aacgccgaag
     5401 gcaaactcta caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg
     5461 cgaactgcca tacccaggac aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcagt
     5521 cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga
     5581 aagcagcgct ggatgcaggc gcgacggaag ccgaaatgaa gccaattcag gacgatatcc
     5641 gtcatgccca gtggcgctgg gatctggcga tcgcttccca cggcattcat atgcacgcac
     5701 cggaagaagg tttacggatg ctcggtacgg cgatggataa agcggcggat gcacgcacca
     5761 aactggcgcg cctgctggcg accaaaggca tcacccatga aatccagatc ccggatatct
     5821 caaccaaaga gaaagcccag caggctattg gcctgaacat ggaacaaatc aaggccgaga
     5881 agcaggactt catcaaaacg gtgatcccgc agtgggaaga acaggcacgt aaaaacggtc
     5941 tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat
     6001 gagcgtatta cgttcgttat taactgccgg ggtgctggcg tcgggcctgt tgtggagcct
     6061 gaacggaatt accgctaccc ctgcggcgca ggcatctgat gatcgctacg aagttaccca
     6121 gcagcgtaac ccggatgccg cctgtctgga ctgtcataag ccagataccg aaggtatgca
     6181 tggaaaacat gcttccgtta tcaacccgaa taacaaactg ccggtcacct gcaccaactg
     6241 tcacggccag ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga
     6301 gccgatgtac aaggttggag agcagaacag cgtctgtatg tcctgtcatc tgccggaaca
     6361 gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg
     6421 ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa
     6481 gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt
     6541 tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc
     6601 gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat
     6661 ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg
     6721 gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt
     6781 agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag
     6841 cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc
     6901 gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc
     6961 gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat
     7021 ttctgccgta aaaccaattt gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg
     7081 acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg
     7141 cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc
     7201 gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat
     7261 cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc
     7321 tggtgacgct ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagta
     7381 cgttgctgcg caccacgctg attgtcgggc cgggcgcggt gatcctcggt ctgttgatcc
     7441 tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta
     7501 cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta catggtggtg ctggtgctgt
     7561 ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc
     7621 tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacat
     7681 tgatgctggt gttggcggtg ttgttggggg cttataccgg ctttctgctg tcggcgctga
     7741 aatcgtatcc gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct
     7801 cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt
     7861 ccacggaagc gcagtttgta caccgtatgg aaatccccgt ggtatggggt gaaatcttcc
     7921 tgctggtggc gttttttgtc ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg
     7981 tggcggcatt aggtggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg
     8041 ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctgccg
     8101 tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt ttcattctct
     8161 acgccgggca gttaacggtg gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt
     8221 cctgaagtcg gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg
     8281 acggcctttg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc
     8341 attctggcgc agttcgcgct cctgttgctc gcctttggcg tactgacgta ttgttttctc
     8401 atcagcgatt tctcggtcat ttatgtcgcc caacatagct acagcctgct gtcgtgggaa
     8461 ctcaagctgg cagcggtgtg gggcggccat gaaggttcgc tgctgctttg ggtgctgctg
     8521 ctttccgcct ggagcgcgct gtttgcctgg cattatcggc agcaaaccga tccgctattt
     8581 ccgctgacgc tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtagtgctg
     8641 tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaatccg
     8701 atgctgcaac atcccggtct tatctttcat ccaccgctgc tttaccttgg ctatggcggt
     8761 ttgatggtgg cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt tgatggtgcc
     8821 tgcgccagaa tttgttggcg ctgggcacta cctggctgga gcgcattaac ggcggggatc
     8881 atcctcggtt cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat
     8941 ccggtggaaa acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagtttg
     9001 tccctgacac gccagcggga gattttccgc cactggtcgc tgttgctggc gatagtaact
     9061 ctgatgctgt cgctactggg taccttaatt gtccgttctg gcattctggt ttcggttcat
     9121 gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc actgattagc
     9181 cttgcgtctc tggctctgta tggctggcga gcgcgggacg gtggcccggt ggtgcgtttt
     9241 tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta
     9301 ctgatcgtgc tggtgggaac gctttatccg atgatttacg gtctgctggg ctggggacgc
     9361 ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac cgtttggcct gttgatgctg
     9421 gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc ggcgctggta
     9481 gctcatgcgg gcgtgctgtt atttgccgct gggatcgtgg tttccagcgt cagccgtcag
     9541 gagatcagcc tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt
     9601 tttgagcgcc tcgatctgca agccaaaggc aattacacca gcgaaaaagc gatagtggca
     9661 ctgtttgacc atcagcaacg cattggtgaa ctgatgccgg agcggcgttt ttacgaagca
     9721 cgtcgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg
     9781 gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc gcttgtatgt acaaagcggt
     9841 gtgcgctgga tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg
     9901 cgggggagga agcgcgatga ataaaggact tctcacgcta ctgctgttat ttacctgttt
     9961 tgcgcgcgct caggtcgtag acacctggca attcgccaat ccgcaacaac agcaacaggc
    10021 gttaaatatt gccagccagt tacgttgtcc gcagtgccag aatcaaaact tactggaatc
    10081 caacgcgccg gtggcagtca gtatgcgcca tcaggtttac agcatggtgg cggaggggaa
    10141 gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa
    10201 cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt tgttactgct
    10261 gatggcactg atcctctggc gagtgagggc gaagcgatga aagagcccaa aataccggtg
    10321 aaaatgctga caacactcac gattttgatg gtatttctct gtgtcggcag ttatctgtta
    10381 agtccaaaat ggcaggcggt acatgcggaa tatcagcgtc agcgcgatcc gctacatcag
    10441 tttgccagcc agcaaacccc ggaagcgcag cttcaggcat tgcaggataa aatccgcgct
    10501 aatccacaaa acagcgaaca atgggcgtta ctgggcgagt actatctgtg gcaaaacgat
    10561 tacagcaatt cgctgctggc gtaccgtcag gcgttgcaac tacgtggtga gaacgctgaa
    10621 ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat gaccgccctg
    10681 actcgcgcaa tgatcgacaa agccctcgcg ctggactcta acgaaatcac cgctctgatg
    10741 ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa
    10801 aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat
    10861 atggcgaaat tgttgcagcg gagatcagat taatcatctg gtgatgaaga ttatgttatt
    10921 gagttgtata aaaaataatc gaatgaacat atgccaaaaa taatcaccaa tcagtattat
    10981 tgcagattaa acaaataaaa atctttccat aacaaatggt tattcattaa tcctgccata
    11041 aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca
    11101 taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa
    11161 ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg cgctccattg
    11221 aggaagttat tctgatgaaa agtataaaat tcagcctggc ctggcagatt ctgtttgcta
    11281 tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc cgcgactggc
    11341 tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc aaaatgattg
    11401 ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt gatgccaaac
    11461 agctcgggcg tattggcgcg aaaaccatta tttacttcga ggtgatcacc accgtcgcca
    11521 tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc
    11581 agctggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta caaagcagtt
    11641 cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg
    11701 cgaaaggcga aatgctgccg atcatcttct tctcggtgct gtttggtctg gggctttcct
    11761 ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca
    11821 tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt gcgctgattg
    11881 cggtgacggt ggctaacttt ggtttctctt ctctgtggcc actggcgaaa ctggtgctgc
    11941 tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg gcgcgcctgt
    12001 gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt ctggcgtact
    12061 ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa gcctacggag
    12121 caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt
    12181 cgacgctgta tcaaagtatt gccgctattt tcatcgctca gctgtatggc attgacctgt
    12241 ccatctggca ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa gggattgctg
    12301 gcgtgccggg cgtgtcgttt gtggtgctgc tggcaacgct gggtagcgta ggtattccgc
    12361 tggaaggcct ggcgtttatt gctggtgttg accgtatcct cgacatggcg cgtactgcgc
    12421 tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa cacaaatttg
    12481 accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat aaaactgcgg
    12541 atcaataatt gaagattgcc ggggttatcc acccggcaat gtgtggatgc ctgatgcgac
    12601 gctggtgcgt cttatcaggc ctaccttaac cgttggttaa tttttcaaac tcttcacacc
    12661 cggtatcaaa cccttccata cagctcaggt tcaaccagtg cagcgccttt tgtttgttct
    12721 tctcgataaa accttcctca ccgtttaaga acatcattcc cgcccagtac tcggaataac
    12781 cggttcggga aattgcagag ctgcgtttga aataccaggt tgccttgtca tcgtcggcct
    12841 taatgcccac gccgttggcg taaatcaaac caagcagcat ttgggcatcg acggcggagt
    12901 cattctccag atcttccgag gcgttttcta acagcgaaat cgcctttgga taatccggtt
    12961 tacccgcttg agtatttacc agaatatgcg ccagcgttac ttcaccttct ttactgcccg
    13021 cttgcgccgc tttctctgca agaacttttg cttgtggata gtccagactg accggattgg
    13081 tgattttgat ctgcgccagc agcgcgcagg catcagcatc accgctgtcg gcggctttct
    13141 gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat
    13201 attgtgcgcg tcggtccccg gcctctgctg cctttaaata ctggctgcca ggctcggagt
    13261 cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc gataattttt ttcattttga
    13321 taatacgatt gggtagggag taaccagtat aaagagttcg aatggataaa aaaacagcct
    13381 ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt
    13441 tcaacttgtt gtactcgtca atcacgtact gctcggcggc gcgctgatcg gcgatcggct
    13501 cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt aagttttcgg
    13561 taaccagctc gttacaggca ccaatccacc actggtaggt catgtaaatc gcccctttgt
    13621 tcggacgatc gctgacctgc gcacgggtga taattttgcc tttacgcgag tgcacccaaa
    13681 ccaatgcctc atcttcaata cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc
    13741 caggttcatc agccagcgcc gccagtgccg cacagttacc ggtcatcgaa cggcaagagt
    13801 agtgaccaac ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg agtttgtcga
    13861 ttggcgctac ccagtcgcag gtgaagaact gtgccagacc gttcggggta tcaaacttct
    13921 ctttaaacag ataagaagtc ccctgatcgg catctgaagt atcgcggcaa ggccactgaa
    13981 tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg cacagatgac
    14041 gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat cggataaccc atacgggtgg
    14101 cgatttcact gatgatttgc cagtccgttt tcagatccca tttcggttca actgctttga
    14161 agaaacgctg gaagccacgg tcagccgcag taaacacgcc ttcatgctcg ccccacgacg
    14221 ttgacggcaa aatgacatcc gccgccgacg cggttttggt cataaagatg tcctgaacga
    14281 taaccagttc cagatcttca aaggctttac gtactgccga cagctccgcg tcagtttgca
    14341 gcggatcttc gcccataatg tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca
    14401 gctcgctgat gcgataaccg gtatgagcag gcaggctttc cacgccccag gctttggcga
    14461 atttctcgcg gttagccgga tctttcacgt actggtatcc cggatacgta tccggcagcg
    14521 cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt acacccgcat
    14581 gcggcttacc gaggttaccg gtcagcatcg cgaggctggt cagagaacgc acggtttcca
    14641 cgccctggta gaactgggtt acacccatgc cccacaggat ggcggcgctt ttcgcctggg
    14701 catacatccg tgccgcctga cgaatctcac tggcgctgac gccggtgata tcttcaaccg
    14761 actccggcgt gtagccttca acgattttac gatactcttc aaagccttct gtacgggaag
    14821 cgacgaacgc tttgtcgtac agattttctt caataatgac atggcccatc gcattcaaca
    14881 gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa
    14941 ttttgcgcgg atcgcagacg ataattttcg ccccgttacg tttagcgtta attacgtgat
    15001 tcgccacgat tgggtgggaa tccgccgggt tgtacccgaa aacgaacact aaatcggtat
    15061 tatcaatttc gttaatagca ttgctcattg cgccattacc gaccgattgg tgcagacctg
    15121 caaccgatgg gccgtgtcag acacgagcgc agcagtcaac gttattggta ccaataacgg
    15181 cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc cgtaccacgc gaggagccgg
    15241 tcgtctggat ggcatccgga ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt
    15301 aattcagtgc ctcatcccag gaaacaggtt cgagtttgcc gccacgctgg cgacggatca
    15361 tgggggtttt caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc cagccataat
    15421 aacccttcag acacagggta ccctggttgg ttttcccctg cgctgcctcc gcccggacga
    15481 ttttgccatt atcgacgacc aggttgattt tgcaacctga tgcgcaatag gggcaaaccg
    15541 tgacgacttt tttcatcggt ctcgctccag ttaatcaaat cacgcatacg cgctctcgac
    15601 tacagtatgc atcttttatg ccacatttta tttggggtca ttccctgata ttacgggcac
    15661 tatttattca aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa
    15721 ataccgcgtg acagccatca cgcggcagac attttatttt ttctcgacga cgggagccgc
    15781 ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc
    15841 cagcatcagt aatgacatct cttcggcaag tactggcaac cgggcttcag tggcctgtaa
    15901 gcggctggtt aagccgcgct gataggcggc ctcggcagcg cgctgggtga agcgcgttgc
    15961 ttccacgcgt tcagcctgca tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt
    16021 gacggcaacg tcacgcaccg cgttcagtac tgactggttg taacgttcaa tcatcatgtt
    16081 gctggcggcg cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa acagcggcaa
    16141 tttcagaccc gggatgaagt tgaactggcg actggttttt ttgaataagg tatccaggtg
    16201 gatggcatca aggccgaaaa acgctttgat atcaaagctc gggtagaaca gcgcccgcgc
    16261 ggaatccacc tgatctaatg acgcctgaac ataccagcgc atggcttgca gatccgggcg
    16321 tctggcgagc aactcataag agagtgtcgc cggaatgccg gtctggactc gtggtaatgc
    16381 caccggtttg atctccggca tatcgctggc ccccgcgcca atcaatgcac gcagagattc
    16441 tcgcgtttcg gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg ccagaatctg
    16501 tgcccgcgcg ccgtggaaag gcacttgtgc ttccagaccg tgcgccactt tactctggtg
    16561 cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct aacagatcga gcatctgata
    16621 gctggcctgc atactgtaat aaagctgcgc tacgcccgtg gtcagcgata gctctactgc
    16681 tgcggtttct gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg
    16741 cacaccccac aaatcaagat ccagtccggc aaacaaacct actgtggctt ccgtatagta
    16801 cggcccgtcc atacccagtg cgggcgcatc catcgcataa gggcttaaaa agccgttcgc
    16861 cgagacgcgt tggcggttga gcatccctaa cgctgcgacc tgtaattgtg aaccggcatc
    16921 taacaaatcg gcctgggact gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg
    16981 tgaaccactt agcgtccgtt ggatcagcgc atccagctgc gggtcattga gttgtttcca
    17041 ccactgcgcc tgcggccagc cggagctggc aagatgaata tcgtcggcca gtttgatttg
    17101 ttccggtttg agctgttgat gaggtgcaga atccttacgt accagggcac agccggaaat
    17161 cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac gattgatcat
    17221 tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc
    17281 aagcgggcga aaagctggca gacgtgttgc tcctgtttta ataaggtggg caggatggcc
    17341 tgcgaggcgg gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc ggttgccaga
    17401 ccggcagcgt atttctccag ggcgtcggca aactgagctg ctctgtccgg ctgtagtgcg
    17461 ttatccagcg cctgcgccga gttccaggtg gcatcccagg cgtgaagaat atcgcggccc
    17521 tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct gtccagttga
    17581 cgctccagcg ccacgcgttg gcacatctct tcacaggcat taaacgccgc atgcagaccg
    17641 atacgaattt gcagataagt gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt
    17701 actttactta acatacccag cgtgccagcc agtttttgcg gcagtgtgcg cgcttcactt
    17761 tcaggccaga caaaggtgta aatcaccgcg gacaccacgg taccaatgat gatacccatg
    17821 gcgcgatcgc gaatttccac caggtcgtac accgggccaa aaacgttttc gagcgtggcg
    17881 agcgcgaagg tgaccaccat ctgtgtgccg atataagaag agcgttcaga gctggtggca
    17941 atccatgcgc ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac aatattgtcc
    18001 agccagggca tgaccagtag cgtgaataac agcgccagaa tcgcgccgca aaaggccccg
    18061 ccaaaacgca gcaccatctt ctggtacgac gaaccgacat ttgggttagc gacgatcacg
    18121 catgtcagca tacaggtgtg aatgccttcc cagtccacgc cgctgtagaa ggtgtaacag
    18181 atcaaacagg cgagcagtgt ttttaccgcg tagcgcatat agtctggatt ggtaaaagca
    18241 tcggcgacca ttgatggcgg tttggcggcg ggcgttggcg gcgtattcgg gtccatctga
    18301 cccagttgta acaacgtctg gcagatattc tccaggttac attcccgtgc cgccatcgct
    18361 tcactttcag tgatccgcca gtcgctttgc cagcactgac cttcagcaac ggcatgctgc
    18421 agcttattga tttctgaggc taatttttgc cgaaattcaa taattgcctg agaatcagca
    18481 aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt tgccacgcag
    18541 ctttgccacc atgcgctttg agttcgccag ttggcatcgt ccgcgagaca aaagacattc
    18601 agtttttgca gcgccagcgc ctctctttca atccgcgttt cgggtagcgg tgcgaggctg
    18661 tccgtcaggt gactaatggc atcatcaagc cgatcattaa gcgcttgatg catttgcgta
    18721 atggcacgac tgggaaacca cagcacgccg attaacgtca tcagcaaggt tggatagagg
    18781 ccaacaacga tacaccacag cgttaagcgc acgaccactt ccggatagtc gagcatggcg
    18841 gggaaggttt gcccgtaaat ggcgacaatg gcgacagcga aaaagaccag tcccagccga
    18901 tgggtgcgca tcaaaaacat acagcccatc aggatcggcc cggcgatgat caatcggatc
    18961 aacggttcgc cgtatgacca tttgtagatc aaaaacaggc tgccgatctc cagcaccgtg
    19021 gcaaccacaa acaagatcgc gacaaatttg gtgtaaaacg cgttcgactg aataccgtaa
    19081 aacagcactg ccagcgataa cgccacaaaa gggatctcaa aagtcatcga gatcagaatc
    19141 accagcaggc agcctaccca gagttgcacg atctgcggca cgcggcctgg tcgccgctcg
    19201 cttaactctt catgaaagaa cgccagcagc ctgaccaccg gtaatggcag ggagttgagc
    19261 gcgctcatta ttgcggctca agattagcga ctgccgaagc gccgatgcgg aacatttccg
    19321 ggtcaggttt atcgaccatg attttgaccg gaaaacgctg ggcaacgcgg acccagttaa
    19381 tagagcgaga aactttcggc aggccgccca gcaccaggcc gccgtcatcc ggtagcacgc
    19441 cgtagccaat cgaatccact ttaccctcga aggttttgcc gctgtcactc atcaggcgaa
    19501 tcgttgcggg tgtacctgag cgaatatttt tcagatcggt ttcgcggaag ttggcgatca
    19561 cgtaccagtg acgagtgtcg attagggtaa aaatagggcg catggcagaa gcaaattgcc
    19621 cgatggaggt tttgagggaa atgacccggc catcaaacgg cgcgcgaacg gtcgccattt
    19681 ccagatgaag tttggtcagg gcaatatccg cttcgaccgc cgcacgttgc gccaccagcg
    19741 catcgacgcc gctgacggcg ctggcggctg actgcgcctg taacaatacg gcattaagat
    19801 cggcttctgc ggcgcgctgc gcagttcttg cacggtcaac gtcttccgct gagacaaaac
    19861 cttctttcag taatggctcg gtgcggcgta atgtatctgt ggcctgtttc gcggcggcac
    19921 gggctttttc taccgtggcg ttaaccgagt cggcaccaaa ctgttgcgcg tcaacgctac
    19981 gctgggtgag cataatttgc ttatccagcg ccgcgaggga ggcttcagct ttcgccagat
    20041 tggcttcgta cgggcgcggg tcaatgcgga acagcaaatc gccctgtttg actgcctggt
    20101 tgtcggtgac cgccagttct acaatgcgac cgctgacttc cggcaccaca tcaatggtat
    20161 ctgctgacgc gtaagcgtca ttagttgatg gcgcactgtc tacgcgccag ataacgaaaa
    20221 caagggcaac cagcgccaac gcaaccacta acagagcagg gaatttactg cgaggagctt
    20281 ttttcggcgt actttccatg agcgcgtctc aaatagatta gaaaaatgcc agccagaaca
    20341 gcatggcgta gagagcaaaa agggcggtat aaataattcc gacaaatgcc agattgatat
    20401 ttgcccgctg aataactcgc ctcgtgatga gcatcaaaat aaggctggca atggcgcaga
    20461 aaaaccagct gggataataa gcaccgatca ccgggattgc cggagataat gagcatcccg
    20521 aaaccatgaa gacgggaatt atccaggctg tttttttgag ttgcatcgcc atgcgagaga
    20581 gaactgttgg catattaaat acatcctgtc tgctgcgaac aatatctgta gtgaatatca
    20641 atatccattg aaggcgtcag tatggctatt ttctcgatga ataaaaatag cttta
//
LOCUS       DAPHDX010000076        20633 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPHDX010000076 DAPHDX010000000
VERSION     DAPHDX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.076"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            62..628
                     /locus_tag="RH058_002808"
     CDS             62..628
                     /locus_tag="RH058_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3138374.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCEQIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANKINESP"
     gene            771..1943
                     /locus_tag="RH058_002809"
     CDS             771..1943
                     /locus_tag="RH058_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDV3138375.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTIVVRVPQGETEDETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            1953..2468
                     /locus_tag="RH058_002810"
     CDS             1953..2468
                     /locus_tag="RH058_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDV3138376.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            2523..2825
                     /locus_tag="RH058_002811"
     CDS             2523..2825
                     /locus_tag="RH058_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDV3138377.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            2840..2959
                     /locus_tag="RH058_002812"
     CDS             2840..2959
                     /locus_tag="RH058_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDV3138378.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            2952..6029
                     /locus_tag="RH058_002813"
     CDS             2952..6029
                     /locus_tag="RH058_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV3138379.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLSELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLSSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGAVLRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPTKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6026..6511
                     /locus_tag="RH058_002814"
     CDS             6026..6511
                     /locus_tag="RH058_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3138380.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKMVGGLL
                     S"
     gene            6508..7608
                     /locus_tag="RH058_002815"
     CDS             6508..7608
                     /locus_tag="RH058_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDV3138381.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSATGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            7699..7917
                     /locus_tag="RH058_002816"
     CDS             7699..7917
                     /locus_tag="RH058_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV3138382.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8153..9838)
                     /locus_tag="RH058_002817"
     CDS             complement(8153..9838)
                     /locus_tag="RH058_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV3138383.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10108..10485
                     /locus_tag="RH058_002818"
     CDS             10108..10485
                     /locus_tag="RH058_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV3138384.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(10515..10772)
                     /gene="grxA"
                     /locus_tag="RH058_002819"
     CDS             complement(10515..10772)
                     /gene="grxA"
                     /locus_tag="RH058_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV3138385.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10932..11219
                     /locus_tag="RH058_002820"
     CDS             10932..11219
                     /locus_tag="RH058_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV3138386.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            11203..11925
                     /gene="nfsA"
                     /locus_tag="RH058_002821"
     CDS             11203..11925
                     /gene="nfsA"
                     /locus_tag="RH058_002821"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV3138387.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11986..12888
                     /gene="rimK"
                     /locus_tag="RH058_002822"
     CDS             11986..12888
                     /gene="rimK"
                     /locus_tag="RH058_002822"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV3138388.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12976..13452
                     /locus_tag="RH058_002823"
     CDS             12976..13452
                     /locus_tag="RH058_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV3138389.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            13803..14915
                     /gene="potF"
                     /locus_tag="RH058_002824"
     CDS             13803..14915
                     /gene="potF"
                     /locus_tag="RH058_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV3138390.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15010..16143
                     /gene="potG"
                     /locus_tag="RH058_002825"
     CDS             15010..16143
                     /gene="potG"
                     /locus_tag="RH058_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV3138391.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIVSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16153..17106
                     /gene="potH"
                     /locus_tag="RH058_002826"
     CDS             16153..17106
                     /gene="potH"
                     /locus_tag="RH058_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV3138392.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAVISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17103..17948
                     /gene="potI"
                     /locus_tag="RH058_002827"
     CDS             17103..17948
                     /gene="potI"
                     /locus_tag="RH058_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV3138393.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18008..18496
                     /locus_tag="RH058_002828"
     CDS             18008..18496
                     /locus_tag="RH058_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV3138394.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            18537..19664
                     /gene="rlmC"
                     /locus_tag="RH058_002829"
     CDS             18537..19664
                     /gene="rlmC"
                     /locus_tag="RH058_002829"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV3138395.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(19839..20570)
                     /gene="artJ"
                     /locus_tag="RH058_002830"
     CDS             complement(19839..20570)
                     /gene="artJ"
                     /locus_tag="RH058_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV3138396.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         4920 a         5058 c         5733 g         4922 t
ORIGIN      
        1 agaaaatgca gaggtaacag ctaactggca tcatctccgg tttttattca gggggatgat
       61 catgcttatt ggctatgtac gcgtgtcaac aaatgaccag aacaccgatt tgcaacgtaa
      121 tgcactgaac tgcgcgggat gtgagcagat ttttgaggac aaaatcagtg gcactaagtc
      181 cgacagaccg gggctgaaaa aactactcag gacactatcg gcaggagaca cgctggttgt
      241 ctggaagctg gacaggttgg ggcgcagtat gcggcatctt gttacgctga tagaagagtt
      301 gcgccagcgt ggtgtgaatt tcagaagcct gactgacagt attgatacca gtaccccaat
      361 gggccgtttc ttttttcatg tcatgggtgc cctggctgaa atggaacgcg aactgatagt
      421 tgaacgtacc agggcagggc tggctgcagc tcgtgccaaa ggcagagtag gtggacgccg
      481 tcctaagttg accaccgaac agtgggcaca gattgggcgt ttactcgagg ccggagaatc
      541 aagacagcgt attgcactga tttttgatgt aggcgtttct accatttata gaaaatttcc
      601 ggcaaataag atcaatgaat ccccctgaat cagcattatg ttgattatcc ctgcaagcag
      661 acaaataccg ttattttgtg tgaataacga cacaactgcg cttagctgtt tgtcaggcac
      721 aatcacttca acatagggcg aagcctaatc caatcaggag gttcgccact atggctcagg
      781 attaccacca cggggtgcgc gttgttgaag tcaacgaagg cacccgatct attaccacgg
      841 tgagcaccgc catcgtgggt atggtctgca cgggcgatga tgccgatgca aaaatgtttc
      901 ctcttaataa acccgtgctg atcactgatg tgctgactgc cagcggtaaa gcgggtgagt
      961 caggtactct ggcccgttcg ctggatgcca tcgctgacca ggcaaaaccc gtgaccattg
     1021 ttgtgcgtgt gccgcagggt gaaacggaag acgaaaccac gaccaatatc atcggcgcag
     1081 tgactgctga aggtaaaaaa acaggtatga aagccctgtt atctgcccag tcacagctcg
     1141 gcgttaaacc gcgcattctc ggcgtgccag gccacgacac caaggcggta gctactgagt
     1201 tgctgagcgt ggcgcaaagc ctgcgtggat ttgcttacct gtcagcgtat ggctgcaaga
     1261 cagtgcagga ggcgatcact taccgcgaaa acttcagcca gcgcgaaggg atgctgatct
     1321 ggcctgactt tactggctgg gacacggtgc tgaatgccga agcaacggca tatgccaccg
     1381 cccgtgcgct tggtctgcgc gccaaaattg acgagcagac cggatggcac aaaagcctgt
     1441 ccaacgtggg cgtgaacggt gtcaccggaa tttctgcaga tgtgttctgg gatctgcagg
     1501 acccggcaac cgatgcaggt ctgctgaacc agaacgacgt caccacgctt gtgcgtaaag
     1561 acggtttccg cttctggggt tcccgctgcc tgagtgatga cccgctcttt gccttcgaaa
     1621 actacacccg cacggcgcag gtgctgatgg acacgatggc agaagcacac atgtgggcgg
     1681 tggataaacc gcttaacccg tcgctggccc gcgacattat cgaaggtatc cgcgccaaaa
     1741 tgcgcagcct ggtcagtcag ggctatctca ttggtggtga ttgctggctg gatgagtcgg
     1801 tgaacgacaa agacacgctg aaagccggaa aactcaccat cgactacgac tacacgccag
     1861 tgccgccact tgaaaacctg atgctgcgtc agcgcatcac cgatcagtac ctggttaatt
     1921 tcgccagcca ggtcagcgcg taaggggaca acatggcttt accacgcaaa ttaaaacacc
     1981 tgaacctgtt taacgacggg aacaactggc aggggatcgt tgagtcgctg acgctgccga
     2041 aatttacccg caaatatgag aagtatcgcg gcggcggaat gccgggtgcg gtggatgtgg
     2101 atctggggct tgatgacagt gcgctggaca cagaattttc cattggtggt actgaattgc
     2161 tgctgtttaa acagatgggt aaagccacgg tggatggcat ccagctgcgc tttaccggct
     2221 ctattcagcg tgacgatacc ggggaagtgc aggccgtgga gcttgtcgtg cgtggacgtc
     2281 acaaagaagt ggattccggc gagtggaaga cgggcgaaag caacaccacc aaagtgacca
     2341 gtaccaacag ctacgcgaag ctgaccatca atggtgaggt gctctatgaa gtggacctta
     2401 tcaacatggt ggaaattgtg gacggtgtgg acctgatgga agcgcaccgc aacgccctcg
     2461 gcctctgata tatctgaacg gcgcgggata ccgcgccaga acccaattga caggacagca
     2521 aaatgagcga taagcagact gaaaagacca ttcaactgga tacccccatc aagcgcggta
     2581 aaacagaaat caccgaaatt gtgctgcgta aaccgcagtc cggtgcgctg cgcggtacac
     2641 gcctgcaggc cattatggat atggatgtaa acgcgatgat gaccgtgatc ccccgcatct
     2701 ccagtccggc actgactgca caggaaattg cagagatgga cccggcagat ctcactgcca
     2761 tgtcggttga ggttgtcact tttttgttga agaagtcggt gcttgccggt ttaccgacag
     2821 cctgacggtt gacgatctgg tggcagatat cgccaccatt tttcactggc cgccatccgt
     2881 tactgacgtt atgccgctga ccgaagtgct ggaatggcgg tataaagcga ttcagagaag
     2941 cggggccaac gatgagtgat aacaacctgc gtctgcaggt cattcttaat gcggttgaca
     3001 agctcacccg cccatttcga tctgcgcagg ccagttcaag agaactggct gctgctgtca
     3061 aaaaatcccg cgatgcaata aagcagcttg atcaggccgg gagcagtctg gacagcttcc
     3121 gaaagctgca ggcagaaaat cagaaattag gcgacaggct gaactatgcc cgccagcgtg
     3181 caaatttgct cagtcaggaa ctgggagcga tggggccgcc ttcgcaacgt caggttgttg
     3241 ctctgggccg tcaacggctg gctgttcagc gcctggaaga acgccagaaa aagctgcagc
     3301 agcagacggc gcttgtgcgt gctgaactgt accgggcggg aatttctgcg aaagacgatg
     3361 cgggagcaac tgcccgttta gcccgtgaaa catcacgtta taaccaggaa ctttcgaaac
     3421 aggaggcgcg gctgaagcga ctgggggaag ctcagcgcag gatgaatgca gcgcgtgcca
     3481 gttatgcccg ttcgctggag gtgcgtgatc gtattgcagg tgccggagcc accaccacgg
     3541 ctgcagggct ggcaatgggc gcaccagtga tggcggcagt aaaaagctat accagcatgg
     3601 aagatgccat gaaaggtgtg gcaaagcagg tcaatggtct gcgtgacgat aatggcaacc
     3661 gcactgcacg tttttatgaa atgcaggatg ccatcaaggc tgccagcgaa cagttgccga
     3721 tggaaaacgg tgctgtggat ttcgctgcac tggttgaagg tggtgcgcgc atgaacgtcg
     3781 caaaccctga cgacagttgg gaagatcaga aacgtgacct gctggccttc gccagtacgg
     3841 cagcaaaggc ggcaacagcc tttgagctgc cagcggatga actgtcagaa agtctgggga
     3901 aaatcgccca gctctacaaa atccccaccc gcaatattga acagctcggt gatgcgctga
     3961 actatctgga tgataacgcc atgtcgaaag gggcagacat cattgatgtg atgcaacgtc
     4021 tgggcggtgt ggctgaccgt ctggattatc gtaaagcggc ggcgctgggt tccaccttcc
     4081 tgacactggg cgctgcgcca gaggttgcag ccagtgcagc aaacgcgatg gtgcgtgaat
     4141 tgtccattgc caccatgcaa agcaaaagtt tctttgaagg gatgaatctg ctgaaactca
     4201 atcctgaagt gattgaaaag cagatgacga aggatgcgat gggaaccatc cagcgcgtgc
     4261 tggagaaggt gaacgcgctg ccgcaggaca agcgcctgtc tgccatgact atgttgtttg
     4321 gtaaagagtt tggcgatgac gcggcgaaac tggcaaacaa cctgtcggaa ctgcagcgtc
     4381 agttaaaact gacagcgggc aatgatgcgc tcggctccat gcagaaagaa tccgacatta
     4441 acaaggattc actttctgcg cagtggttgc tggtcaaaac cggagcgcag aacaccttca
     4501 gcagcctggg cgaaacgctg cgccagccgc tgatggatat tctgtacacg gtgaaaagcg
     4561 tcacgggggc gttgcgtcgc tgggtggaag ctaacccgga actgacaggc acactgatga
     4621 aagcatcggc tgttgtggct gcggttaccg tcggcctcgg caccttagcg gtggcgctgg
     4681 ctgcagtgct ggggccgctg gcagtgatcc gtctgggatt ctctgtgctg ggtatcaaaa
     4741 cgttatcttc cgttacggca gcagtaaccc gaaccagcag cgcgttgtcc tggctggctg
     4801 gcgcaccact ggcactgctg cgacgcgggc ttgcttcatc gggcaacgcc gcaggtttac
     4861 ttactgcgcc gttatcgtct ttgcgccgca cggcatcact gacgggaaat gtccttaaaa
     4921 ctgtagcagg tgcgccggtt gcacttttgc ggtctggatt atccggttta cgtgctgttg
     4981 ctgtgatgtt tatgaatcct ctggcggtac tgcgcggtgg actggctgcc gcaggcgcgg
     5041 tgcttcgtgt gcttgcatct ggtccgctgg cgatgctgcg cgttgccctg tatgccatat
     5101 ctggtctgtt aggtgctctg ctcagtccga taggtcttgt ggttactgca ctggcgggcg
     5161 tggcgctggt tgtctggaaa tactggcaac ccatcaccgc atttctcggt ggcgtggtgg
     5221 aaggattcaa agcggcggca ggtcccatca gtgctgcatt cgaaccactt aagcctgtgt
     5281 ttcagtggat tggcgacaaa gtacaggcgc tgtggggctg gtttactgat ctgctgacgc
     5341 ccgttaagtc gacctctgcc gaactgcaga gtgcagcggc aatggggcgg cgattcgggg
     5401 aggcactggc ggaagggctg aatatggtca tgcatccgct ggactccctg aaatccggcg
     5461 tttcctggtt gctggagaag ctcggcattg tcagtaaaga ggccgcaaag gcaaaactgc
     5521 cggaaagcgt gacgcgtcag caacctgcga cggtgaatgc agacggtaaa gtgatgatgc
     5581 catcgggtgg ttttccatca tggggatatg gctttgcggg gatgtatgac agcggcgggt
     5641 atatcccgcg cgggcagttt ggcatcgtcg gtgaaaacgg gccggaaatt gttaacggcc
     5701 cggcaaatgt gaccagccgg agaaatacag ctgcactggc tgccgttgtt gccggaatga
     5761 tgggcgttgc tgccgcgcca gcagagcttc caccgttgca ccctttggca cttcccacga
     5821 aaggtggaga agcaattgtg agtcgcgcag ccactgtgcc gctcgttcaa cggattgagg
     5881 caccgacgca gatcatcatt cagacgcagc caggacaaag tgcgcaggat attgcgcggg
     5941 aggtggcacg ccagcttgat gaacgtgaac gcaggctgaa ggcaaaagcc aggagtaact
     6001 acagcgatca ggggggatac gacgcatgat gatggtgctg ggattgtacg tgtttatgct
     6061 gcgcaccgtt ccgtatcagg aactgcagta tcaacgcagc tggcgacatg cggcaaacag
     6121 tcgggtaaac cgacgtccgt ccacgcagtt tctgggaccg gaaaacgaca tgctgacgct
     6181 ttccggtgtt cttatgccgg aaataacagg cggcaggctg tcgttgctgg cactggagca
     6241 gatggcagaa caggggaaag catggcccct gattgaaggc agcggcacga tttacggcat
     6301 gtatgtgatt gagggactga atcagactaa aacggagttt ttccgcgacg gtatgccgcg
     6361 ccggattgag ttcaccctgt cgctcaaacg agtggatgaa tccctgtccg atatgttcgg
     6421 tgatctcagt gcgcaactga ataatctgca ggacacggca acgtctgcct taagcgatat
     6481 cagtaaaatg gtgggagggc tgctgtcgtg aatttcagct ctgaactgct taacaaaggc
     6541 aacaaaactc ccgcattcag catcagtatt gagggcaggg atatcaccac tgtgctggat
     6601 aaccgcctga tgagtttgac gctgacggat aaccggggct ttgaagcgga ccagcttgat
     6661 ctggagctgg atgacgctga cggaaaaatc gtgctgccgc gccgtggtgc ggttattacg
     6721 ctggcgctgg gctggaaggg gcagccgctt ttcccgaaag gggcattcac agtggacgag
     6781 attgaacaca ctggcgcacc ggaccgcctg actatccggg cgcgaagtgc tgattttcgg
     6841 gaaacgctga atacccgccg tgaaaaatcg tggcataaga ccaccgtcgg ggaagtggtg
     6901 aaggaaatag ccgcgcggca caagctgaag atggcactgg gtaaagatct gtcggataag
     6961 cccgtggagc atatagacca gactaatgag agtgacggca gttttctgat gcggctggcg
     7021 cgacagtacg gtgccatcgc gtcggtgaaa aatggcaatc tgttattcat ccggcagggg
     7081 cagggcaaaa gcgccactgg taaacctctg ccagtgatca ctatcacacg caaggacggc
     7141 gacagtcacc gatttaccct ggcagatcgc ggagcctaca cgggcgtaat tgccagctgg
     7201 ttgcataccc gcgaacctgc gaagaaagaa agcaccacgg tgaagcgtaa gcgcagaact
     7261 aagaagcaga agaaagagcc agaagcgaag cagggcgatt acctggtggg tacggatgaa
     7321 aacgtgctgg tacttaatcg cacttatgcc aaccggagca acgccgaacg agcggcgaaa
     7381 atgcagtggg aacgcctgca acgcggcgtt gcgtcatttt cgctacaact ggcagaaggg
     7441 cgggcagatc tctacacgga aatgcctgtg aaggtcagtg gttttaaaca gccgatagat
     7501 gatgcggaat ggaccattac gactctgaca cataccgtca gcccggataa cggttttaca
     7561 accagtctgg agcttgaagt gaggattgat gatttcgaaa tggaatgatt cttcgcaatg
     7621 gagaactttt aagtttgcaa aatggaataa tgcggtatca ttattgtgaa tttagcaaaa
     7681 atggggagag ctcgaaaaat gatgatttgc ccactgtgtg gaagtgccgc ccatactcgc
     7741 agcagttttc aggtatcttc attgaccaaa gagcgttaca accagtgcca gaacattaac
     7801 tgcagccata cttttgttac ccatgaaact tttgttcgtt cgattgcaac gccaaaagag
     7861 tcaaatccgg ttcagccgca tccaatgaaa tcaggacagg tggcgctctc tctttgacgc
     7921 tgccgccatt ttgtcgccat cgttaaaaaa cagtgtttct aacatcatga ttttaaacag
     7981 cttaaatttc aggcaacaaa aaacccatca accttgaacc gaaatggcgg ggttgatggg
     8041 ctccacaaaa tggggacatc aaagaaaagc agtggcacta attaagactg atgccctgcg
     8101 gaaaagttct gcggttgtgc aaaaaaattt cattttcagg gcaacttcag ttttatccta
     8161 atcctggcca taccatgacg atgattgtcc ctgccagcgt cagcaggacg ttggcgattg
     8221 cgtaggtgcc cgcgtagccc agcgcaggga tgttactgcg agctgtatca ctgatgatct
     8281 ccattgccgg cgcgcaggtg cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca
     8341 ttcgcaatac ataagcaccg aacaagaaac agataaccac gggcacaaga ctgacaatta
     8401 atccggcaat caacatctga ccgccaatcg cgcccaggcc gttattaata ccgctaccgg
     8461 cgctcagacc aacgcctgcc ataaacacca tcaagccgaa ctctttcacc atgcttaatg
     8521 caccttgcgg aatgtaaccg aaggtcgggt ggttagcacg cataaagccc agcataattc
     8581 cggcgaataa caacccggca gcgttcccca tgccgaaact gaatgtgctg aactggaagg
     8641 tgatcatccc gatcatcagc ccaataacaa agaaggcgca aaatgccagc aggtcagtga
     8701 cctggctgtg aatcgagata aagccgatgc gatcggcgat ggtttttacg cgacgggcat
     8761 caccgctgac ttgtaaaacg tcacctttgt taagcacgac gttgtcatct atcggcatct
     8821 caatctggct acgaatgacg cggttaagga agcaaccgtg atcggtcaac ttcagttgtg
     8881 cgagacgttt acctacagcg ttatggtttt taacgaccac ttcttcagtg acgatacgca
     8941 tgtcgagaag gtcacgatcg aaaacttctt taccgttacg gaagctggga tcgagtcggg
     9001 catgggcgtc gggatagcct accaacgcta tttcatcgcc catttgtagc acggcatcac
     9061 cgtctggatt tgccagaatc ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc
     9121 gataaatacc cagttcacgc agatttttgc cgtcggtcca ggccaccagc tccgggccga
     9181 cgcgataggc gcggatcacc ggtaaataaa ccttacggtt ggcatcagtg tccaggccac
     9241 gttcgcgggc gatttgctgg gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt
     9301 aacgcgcacc aacaatcaaa ctcaccagac cgattaaata ggttaaggca tacccgaggc
     9361 tcagattatc cagtgccagt gagagctgcc tgctttccat gccggaatga cgcagtgtat
     9421 cgccagcacc gaccagaacc ggtgtcgacg tcatagagcc tgctaacata ccggccgtca
     9481 ggccaatatc ccagccaaac agcttaccta accctaaggc gatcaccagc gcactgccaa
     9541 ccatcaccag tgctaacatt aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt
     9601 tcggtccggc ttcgaccccg acgcagaaaa taaacagcat aaagccaaga ttaagcgcat
     9661 cggtgttaat gctgaaatgt tgttggccta ataacagcga tacgactaaa acgccaatgg
     9721 aattacccag ttggatcgaa ccaagtcgta actttccgag acatagccca agcgcgagga
     9781 ccacaaataa taacagaatg taattcccat ttaacaattc ggcgacgttt atattcacgg
     9841 aggctaactt cttgtttact agtaagctgt tgaaagaaat ggtaatttac gataatgttt
     9901 tttaccagaa ttcagggcgc agattcattc agcgcaccta aacgatagta aagtaacaat
     9961 atattttact agtgtaatca cattaggtat caacggctat atgaattgcg ttggcctata
    10021 ttagcatgga atgcgaagcg gctttatctt actgaacgcc acactggcga aaaatgtgtt
    10081 cgatagacgc agtgtcagga ggaacgagtg aaacataaac aacgttgggc gggggcaatc
    10141 tgctgttttg tcctcttcat tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct
    10201 tttcgggctg ccgggcatcc tgaaatcggc ttgctatttt tcattcttcc tggagcagtc
    10261 gccagcttct tttcacagcg tagagaagtc ctgaaacctc tgtttggcgc aatgctggcg
    10321 gcaccctgtt cgatgctcat tatgcggctg tttttttcac cgacgcgctc attctggcaa
    10381 gagctggcat ggttactaag cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta
    10441 tttatcagta gtttgtttaa accacagcac agaaaaaatc agtaaagccc tcaacgcgag
    10501 ggcttgtcag acgatcaggc gtccagattt tctttcaccc atgcagcaaa atcggtatag
    10561 ccgccgatat gttgctgatc gacaaaaatc tgcggcacgg tttctacggg tttacctgcc
    10621 ttttgttgta gatcttcttt agtgatccct tccgcacgaa tatctacata ctgatactga
    10681 aaatcatcgc gttcattgct caatttctca gccagatctt ttgcacgcac acagtaaggg
    10741 caacccggac gaccaaaaat aacggtttgc attatttctc tcctcataga tttatgcctg
    10801 taatgatcac gctaaaatgt attcgctgaa agtaggttta acctgttgca ttaattgcta
    10861 aaagctataa ctgttaaaca caatacagtg aaaagtttta gactgaaggc tcactttgca
    10921 gagggaagcg tatgcgcgcg atcggtaaat tgcctaaaag cgtgttgata ctggaattta
    10981 tcggaatgat gttgctggcg gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg
    11041 agccattttc tcggccagaa gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc
    11101 ttcccgctgc ggtggtggtt attcttcagg tggcaaaacg tcttgcccca cagctgatga
    11161 accgtccacc gcaatattca cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa
    11221 cttatttgtg gccatcgctc cattcgccat ttcactgatg aacccatttc cgaagcgcag
    11281 cgtgaggcga ttattaacag cgcccgtgcg acgtccagtt ccagtttttt gcagtgcagt
    11341 agcattattc gcattaccga caaagcgtta cgtgaagaac tggtgacgct gaccggcggg
    11401 caaaaacacg tagcgcaagc ggcggagttc tgggtgttct gtgccgactt taaccgccat
    11461 ttacagatct gtccggatgc tcagctcggc ctggcggaac aactgttgct cggtgtcgtt
    11521 gatacggcaa tgatggcgca gaatgcatta atcgcagcgg aatcgctggg attgggcggg
    11581 gtatatatcg gcggcctgcg caataatatt gaagcggtga cgaaactgct taaattaccg
    11641 cagcatgttc tgccgctgtt tgggctgtgc cttggctggc cagcggataa tccggatctt
    11701 aagccgcgtt taccggcctc cattttggtg catgaaaaca gctatcaacc gctggataaa
    11761 ggcgcactgg ctcagtatga cgagcaactg gcggaatatt acctcacccg tggcagcaat
    11821 aatcgccggg atacctggag cgatcatatc cgccgaacaa tcattaaaga aagccgccca
    11881 tttattctgg attatttgca caaacagggt tgggcgacgc gctaaaaccg ccacgtcgat
    11941 gtatgatacg cgggcttttg accaggtctg acagagaggt gcagggtgaa aattgccata
    12001 ttgtcccggg atggaacgct ctattcgtgt aagcggctgc gtgaagccgc tatacagcgc
    12061 ggtcacctgg ttgaaattct tgatccgctt tcttgctaca tgaacataaa tcctgcggcg
    12121 tcttctattc actacaaagg ccgcaagtta ccccattttg acgcagtgat cccgcgtatt
    12181 ggcaccgcca ttacctttta tgggacggcg gcactgcgcc agttcgagat gctggggagc
    12241 tatccgctca atgagtcggt cgccattgcc cgggcgcgtg acaaattgcg ttccatgcaa
    12301 ctgctggcgc gtcagggcat cgacctgcct gtcacgggca ttgcgcattc gccggatgat
    12361 accagcgatt taatcgacat ggtcggtggt gcgccgctgg tggtcaagct ggttgaaggc
    12421 acgcagggaa ttggcgtcgt gttggcggag acgcgtcagg cggcggaaag cgtgattgac
    12481 gctttccgcg gtctgaacgc gcatattctg gtgcaggaat atatcaaaga ggcgcaaggg
    12541 tgcgatatcc gctgtctggt tgttggtgat gaagtggtcg ctgcgattga acggcgagcg
    12601 aaagagggcg attttcgttc caatttgcat cgtggcggcg cggcaagcgt cgccagtatc
    12661 acaccacagg agcgtgaaat cgcgataaaa gccgcgcgaa cgatggcgct ggacgttgct
    12721 ggtgtggata ttctgcgtgc taatcgcggg ccgttggtga tggaagtgaa tgcgtcgcca
    12781 gggctggaag gaatagaaaa aacaaccggt atcgacatcg cgggtaaaat gatccgctgg
    12841 atcgaacgcc acgctacgac ggaatattgc ctgaaaacgg gtggttagtc gcaatcacat
    12901 tactgatcat ggttttgcct gcgctttttg cgtaagctgt gccggtcttt ttatcggaag
    12961 aggttgtaca aaattatgac atcgctggtc gttcctggtc tggatacgtt gcgtcaatgg
    13021 ctcgatgacc tggggatgag tttttttgaa tgtgataact gtcaggctct gcatctgccc
    13081 catatgcaga atttcgacgg tgtctttgat gccaaaatcg atctgatcga taacacgatc
    13141 ctgttttctg ccatggcgga agtccgacct tcagccgtat tgccgctggc ggcggattta
    13201 tctgccatca atgctagttc gctgaccgtg aaagcatttc ttgatatgca ggatgataat
    13261 ctgccaaagc tggtggtttg ccagtcttta tccgttatgc agggcgtaac ctatgagcag
    13321 tttgcatggt tcgtgcgtca gagcgaagag cagatttcga tggtcattct tgaagctaat
    13381 gcccatcaac tgctgttacc gactgacgat gaagggcaaa acaacgttac cgaaaactat
    13441 ttcctccact gataactcct ttcgagcacg cagtcgctgg tgcagtggct gcgcgctgca
    13501 aaattatctg ctgtttttaa ccttttctta aagattgttt cacttatctt gtgtcgattt
    13561 ggctttatca catagagcaa atacgcataa aaatttgtta aataccgttt tttaatccga
    13621 gctatagtct caaaccctgg ctaaagttat tcttgcgatg cttttatata gcgagcagtg
    13681 ctggccggga gagagttctc ttttcttaca ccgcgccgat aaaaaatatg cacgtttatt
    13741 gcatatcttt cagtgtgaca acttttgttc gtttgttaac gaactttcag aaggaaagag
    13801 atatgaccgc cttaaataaa aaatggctat cgggtctggt tgcgggtgct ctgatggccg
    13861 tctctgtcag cacgctcgcg gctgaacaaa aaacactcca catttataac tggtctgatt
    13921 atatcgcccc ggacacggtg gccaattttg aaaaagaaac cggtattaaa gtcgtctacg
    13981 atgttttcga ctctaacgaa gtactggaag gcaaattaat ggcggggagc accggctttg
    14041 acctggtggt gccgtcagca agctttcttg aacgccagct gacagcggga gttttccagc
    14101 cgctggacaa aagcaaattg ccggagtgga agaatctcga cccggaactg ctgaagttgg
    14161 tcgccaaaca cgatcccgac aataaatttg ctatgcccta tatgtgggcg acgaccggga
    14221 ttggctataa cgtcgataaa gtcaaagcgg tgctgggcga aaacgcgccc gtcgatagct
    14281 gggacttgat cctcaagcct gaaaatctgg aaaaactgaa aagctgtggc gtctctttcc
    14341 tggatgcgcc tgaagaagtt tttgctaccg tgctgaatta tctcggcaaa gatcccaaca
    14401 gcactaaagc ggatgattac accggaccgg caaccgatct gctgttaaag ctgcgcccga
    14461 acattcgtta tttccattca tcgcaataca ttaacgacct ggcaaacggc gatatttgcg
    14521 tcgctatcgg ctgggcaggc gatgtctggc aggcgtcaaa ccgcgcgaag gaagcgaaga
    14581 atggcgtgaa tgtctctttc tcgattccga aagaaggggc gatggcgttc tttgatgtct
    14641 tcgccatgcc tgcggatgcc aaaaacaaag acgaagccta tcagttcctg aattacctgc
    14701 tgcgcccgga tgtagtagcg catatttccg accatgtgtt ctatgccaac gccaataaag
    14761 cagccacgcc gctggtgagt gcggaagtcc gtgagaaccc gggtatttat ccgcctgcgg
    14821 atgttcgtgc gaagctgttc actctgaaag tgcaggaccc gaaaatcgac cgtgtgcgca
    14881 cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc agccttagat gccgggcggg
    14941 cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc ttgcacacat tcaatgccgg
    15001 agagctgccg tgaatgacgc tatccctcgc ccccaggcga aaacccgtaa ggcgctgacg
    15061 ccgctattag aaatccgcaa cctgaccaaa tcctacgatg gccaacatgc ggtggatgat
    15121 gtcagcctga ccatctacaa aggtgaaatc ttcgcgctac tgggcgcatc cggctgtggc
    15181 aagtccacgc tgctgcgtat gctggcaggt tttgaacaac cttctgccgg acagataatg
    15241 cttgatggcg tcgatttgtc acaggttccg ccttacctgc gccccatcaa tatgatgttt
    15301 cagtcttacg cgctgtttcc gcatatgacc gtggaacaga acatcgcttt tggcctgaaa
    15361 caggacaaac taccgaaagc ggaaattgtc agccgggtca atgagatgct cggcctggtg
    15421 catatgcagg agttcgccaa acgcaaaccg catcagcttt ccggtggtca gcgacagcgt
    15481 gtggcgctgg cccgaagcct tgccaagcgc ccgaaactat tactgctcga cgagccgatg
    15541 ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga tattctggag
    15601 cgtgtcggcg tgacctgtgt gatggtcact cacgatcagg aagaggcgat gacgatggcg
    15661 ggacgtatcg ccattatgaa tcgcgggaaa tttgtccaga ttggcgagcc agaagagatc
    15721 tacgaacatc caaccacccg ctacagtgct gaatttatcg gttcggttaa tgtctttgaa
    15781 ggtgtactca aagagcgtca tgaggatggg cttgtgcttt attcgcctgg gctggtgcat
    15841 ccactgaaag tcgacgcgga tgcctcggtg gtcgataacg tgccggtaca tgtggcgctg
    15901 cgcccggaaa aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa cttcgcggtg
    15961 ggggaggtga tacacattgc ctatctcggc gatctttcgg tgtatcacgt tcgtctgaaa
    16021 agtgggcaga tgattagcgc ccagctacaa aacgcccatc gtcatcgtaa agggttaccg
    16081 acctggggcg acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt gctgacggtt
    16141 taaggagcaa agatgagtac acttgaacct gctgcccagt cgaaaccacc gggcggattt
    16201 aagctgtggt tgtcgcagct gcaaatgaag catgggcgca aactggtcat tgcgttgccg
    16261 tatatctggt tgatcttgct gtttctgctg ccatttctga ttgtctttaa aataagcctg
    16321 gcagagatgg cgcgcgctat tccaccttat accgaactga tggagtgggc tgacgggcaa
    16381 ctttccatca ctcttaatct cggtaatttt ctgcaactga ccgacgatcc gctctatttt
    16441 gatgcttatc tccagtcgtt acaggtagcg gtgatttcga ctatttgttg tttactgatc
    16501 ggctatccgc tggcgtgggc ggtggcgcac agtaagccct cgacccgtaa tatcttatta
    16561 ctactggtga tcctgccgtc gtggacctcg tttctgatcc gcgtttatgc ctggatggga
    16621 atattaaaaa acaacggtgt gctgaataat tttctgctgt ggctgggggt tatcgatcaa
    16681 ccgctgacca ttctgcatac caatctggcc gtttatatcg gcattgttta cgcttacgtg
    16741 ccgtttatgg tactgccgat ttataccgcg ttgattcgta ttgattattc gctggtggaa
    16801 gcagcgctgg atctcggtgc acgaccgctg aaaacgttct ttactgtgat cgtgccgctg
    16861 actaaaggtg ggattattgc cggatcgatg ctggtgttta tcccggctgt gggcgagttt
    16921 gtgatcccgg aactgctcgg tggcccggac agcatcatga tcgggcgcgt gctatggcaa
    16981 gagtttttca ataaccgcga ctggccggtg gcctcggcgg tagcgatcat catgttgctg
    17041 ctgctaattg tgccgataat gtggttccac aaacaccagc aaaaaagcgt gggagaacac
    17101 ggatgaataa tttaccggta gttcgttcgc cctggcggat tgtgattttg ctgctgggct
    17161 ttacctttct ctacgcgcca atgctgatgc tggttatcta ttcgtttaac agctcgaagc
    17221 tggtgacggt gtgggccggc tggtcaacgc gctggtatgg tgagttattg cgcgatgacg
    17281 cgatgatgag tgcggttggt ttaagcctga caattgctgc ctgtgcggca acggcggcgg
    17341 cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt tggcaggttt cgcggatcaa
    17401 atggctttgc ctttatgatc accgcgccgc tggtgatgcc agatgtcatc acgggcttgt
    17461 cgctgttgtt attattcgtc gcgcttgctc atgccattgg ctggcctgcg gaccgcggta
    17521 tgctcaccat ctggctggcg catgtcactt tttgtacggc ttatgtggcg gtcgttattt
    17581 cttcaagatt gcgggaactg gatcgctcga tagaagaagc agcgatggat ctcggtgcga
    17641 cgccgctgaa agtgtttttc gtcattacgc taccgatgat catgcccgcg atcatttctg
    17701 gctggttact ggcttttact ttgtcgcttg atgatctggt gatcgccagc tttgtttccg
    17761 ggccgggagc caccacgtta ccgatgctgg tcttttccag cgtgcggatg ggggtgaatc
    17821 cggaaatcaa cgcccttgca acgttaattc tcggtgcggt cggaattgtc ggatttatcg
    17881 cctggtatct gatggctcgc gcagaaaaac agcggatacg cgatatccag cgtgcaagac
    17941 atggctgaag acactaaaat ttgccaacct ggctacataa tgccgcgcat gccgcggcat
    18001 tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa acatcttcat ctcatgctcg
    18061 cctgaatgtg cctgcgctgg tgcaggtggc ggcgctcgcc attattatga tccgtggcct
    18121 cgacgtgctg atgattttca atacgctggg cgtgcgcggt attggcgagt tcattcatcg
    18181 cagcgtacaa acctggagtt taacgctggt ctttttaagc agtctggtgc tggttttcat
    18241 tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct ggctatatct
    18301 gctgacacaa atcaccgccg caagttactt gtgggcggct tcgctggggt acggttatcc
    18361 ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc tgatgctgca
    18421 gaagctgccg gatatgctca tcctgatgct gctgttcgtt ccctcgacca gtcggcggtt
    18481 cttccagttg caataatgtg tataatcgtc gcccctgatg atgtgaaggt caatgtatgc
    18541 agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg cagccgattc
    18601 cagagcaact ctccgctaaa accgccgatc ttaaaaatct gctcgccgat tttccggttg
    18661 aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa gccaaaatgg
    18721 tggtgagtgg tagcgttgaa aaaccactgc tcggtatgct gcatcgcgac ggcacaccag
    18781 aagacctttg tgactgcccg ctttatcctg cctcatttgc gcccgttttt gcggcgctaa
    18841 aaccgtttat cgcccgtgcg gggctgacac cctacaacgt ggcgcgtaag cgtggcgaac
    18901 tgaaatacat tctgctgact gaaagccaga gcgatggcgg catgatgctg cgctttgtac
    18961 tgcgttctga aaccaaactg gcacaactgc gtaaggcgct gccgtggtta caggaacaac
    19021 taccgcaact gaaagttatt accgtcaata ttcagccggt acatatggcg attatggaag
    19081 gggagacgga gatctacctt actgaacaac aggcgctggc agagcgtttt aatgacgtac
    19141 cgctgtggat ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc agccagctgt
    19201 acgctaccgc gcgcgactgg gtacggcagc tgccagttaa acatatgtgg gatctgttct
    19261 gcggtgtggg tggctttggt ttacactgcg cgacgcctga catgcagtta accgggatcg
    19321 aaattgcatc agaggccatt gcctgtgcaa agcagtcagc cgctgaactg ggcttaacgc
    19381 gtttgcaatt tcaggcgctg gactccactc agtttgccac cgctcagggg gaggtgccgg
    19441 agctggtgct ggttaatccg ccgcgccgcg gcattggtaa accgctatgt gattatctct
    19501 caacaatggc accgcgtttt attatctact ccagctgtaa cgcccaaacc atggcgaaag
    19561 atatccgcga actgccaggt taccgcatcg aacgggtaca gctttttgat atgttcccgc
    19621 acaccgcgca ctatgaagtg ctgacgctgc tggtgaagca ataaaaaagc cgcaggtgcg
    19681 gcttcagatt gctgatcagg tgcgctttgt tcatgccgga tgcggcgtgt acgccttatc
    19741 cggcctacaa aatcatgcta aatcaataca ttgcagagtt gatgtgggcc ggataagcgc
    19801 agcgcatcag gcaatcttac ttttgtcatc agtctcaatt actgcggaaa ccactggtca
    19861 ttgattttct gatatgtacc gtcagcttta attgctgcca gcgcgttatt cagtttttcc
    19921 agcagagctt tgttatccgg acgtacagcg atgcccagac cggtgccgaa gtattgcgga
    19981 tcggtcactt tttcagtagc aacgcccagt tgcggattgg ttttcagcca ttcgtttacc
    20041 accgctgtgt caccaaatac cccatcaata cgaccatttt tcagatcgat aaaggcattc
    20101 tgataactgt cataggagac agttttcact tcggggtgct gatcctgaat atatttctgg
    20161 tgcgtggtgc cgttttccat cccaatacgt ttgcctttca gatcggcaaa cgttttgtag
    20221 gtatcttttt tggcaatcac gacggctgag ttttcatagt agggcgtggt aaacgatacc
    20281 tgcttgctac gctcgggcgt gatatccata ccggagatta cggcgtcata ttttctgaat
    20341 ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa aagtacattc tgcctgcatt
    20401 tgtttgcaca aggcttttgc cagatcgata tcaaagccga caatctcatt attagcacct
    20461 atagattcaa agggtggata ggtggctgaa acgccaaaat tgattttctc tgcggcagaa
    20521 gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta actttttcat gatggaactc
    20581 ccgtctgtca atcttatgat ttttggccgt gtctgcggca tgggataaca atg
//
LOCUS       DAPHDX010000077        20144 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPHDX010000077 DAPHDX010000000
VERSION     DAPHDX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.077"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(26..784)
                     /gene="aroD"
                     /locus_tag="RH058_002831"
     CDS             complement(26..784)
                     /gene="aroD"
                     /locus_tag="RH058_002831"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV3138397.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(815..1681)
                     /gene="ydiB"
                     /locus_tag="RH058_002832"
     CDS             complement(815..1681)
                     /gene="ydiB"
                     /locus_tag="RH058_002832"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV3138398.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1693..2958)
                     /locus_tag="RH058_002833"
     CDS             complement(1693..2958)
                     /locus_tag="RH058_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138399.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3185..4399)
                     /locus_tag="RH058_002834"
     CDS             complement(3185..4399)
                     /locus_tag="RH058_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138400.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4499..4855)
                     /locus_tag="RH058_002835"
     CDS             complement(4499..4855)
                     /locus_tag="RH058_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV3138401.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4991..5098)
                     /gene="rprA"
                     /locus_tag="RH058_002836"
     ncRNA           complement(4991..5098)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RH058_002836"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5284..6396)
                     /gene="ydiK"
                     /locus_tag="RH058_002837"
     CDS             complement(5284..6396)
                     /gene="ydiK"
                     /locus_tag="RH058_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV3138402.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6785..9841
                     /locus_tag="RH058_002838"
     CDS             6785..9841
                     /locus_tag="RH058_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV3138403.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRYVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9838..10248
                     /gene="menI"
                     /locus_tag="RH058_002839"
     CDS             9838..10248
                     /gene="menI"
                     /locus_tag="RH058_002839"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV3138404.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10348..10536
                     /locus_tag="RH058_002840"
     CDS             10348..10536
                     /locus_tag="RH058_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV3138405.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11084..11452
                     /gene="sufA"
                     /locus_tag="RH058_002841"
     CDS             11084..11452
                     /gene="sufA"
                     /locus_tag="RH058_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV3138406.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11461..12948
                     /gene="sufB"
                     /locus_tag="RH058_002842"
     CDS             11461..12948
                     /gene="sufB"
                     /locus_tag="RH058_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV3138407.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12958..13704
                     /gene="sufC"
                     /locus_tag="RH058_002843"
     CDS             12958..13704
                     /gene="sufC"
                     /locus_tag="RH058_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV3138408.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13679..14950
                     /gene="sufD"
                     /locus_tag="RH058_002844"
     CDS             13679..14950
                     /gene="sufD"
                     /locus_tag="RH058_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV3138409.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14947..16167
                     /gene="sufS"
                     /locus_tag="RH058_002845"
     CDS             14947..16167
                     /gene="sufS"
                     /locus_tag="RH058_002845"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV3138410.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16180..16596
                     /gene="sufE"
                     /locus_tag="RH058_002846"
     CDS             16180..16596
                     /gene="sufE"
                     /locus_tag="RH058_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV3138411.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16745..17749
                     /gene="ldtE"
                     /locus_tag="RH058_002847"
     CDS             16745..17749
                     /gene="ldtE"
                     /locus_tag="RH058_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV3138412.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17813..18049)
                     /gene="lpp"
                     /locus_tag="RH058_002848"
     CDS             complement(17813..18049)
                     /gene="lpp"
                     /locus_tag="RH058_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV3138413.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18360..19772)
                     /gene="pykF"
                     /locus_tag="RH058_002849"
     CDS             complement(18360..19772)
                     /gene="pykF"
                     /locus_tag="RH058_002849"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV3138414.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5267 a         4861 c         5145 g         4871 t
ORIGIN      
        1 ggtgttcccg ccgaaatatt attgcttatg cctggtgtaa aatagttaat accgtgcgta
       61 aatcatttac cgagatttgc cctggtgcag aggctttttt taccgcacca aaagttgccg
      121 ccgagccaaa tacttcacca gccagacgag aaattacgcc agtttttgcc atcgacatcg
      181 tgatgattgg acgatcggca tactgctcct gcatctccag ggtcgcggta agcaacgtca
      241 gcacatcgct ggtactttgc ggcatcagcg caatcttagg aatatcggcg tcgaaggact
      301 gcattttgcg cagacgggca atgatttctt cggcttccgg cgttttatgg aagtcatggt
      361 tggacatgac aactttcaca tcatgcgcgt gggcgtaggc gacggtttct ttaacctgat
      421 catcgccggt aaataactcc agatcgatca tatcaaccag gccgctgtcg atggctgcac
      481 gattgagagc gatataagcc tcggtggaaa tcgcctgctc gccgccttct ttggcactgc
      541 ggaaggtaaa cagcagcggt ttttctggca tggtttcacg gagaattttt gccgccgcca
      601 tgacagactc cacattggag aggtcggcaa agtggtccac acgccattcc agaatatcaa
      661 agtccgcttc acgataggcg agagcttcgg atttcacgcg ggcgatatct ttcgccatca
      721 gcgagacgat gattttaggt gcgcccgtac caatgacgag atcttttaca gttacggttt
      781 tcatttttta ccctttctgc acgcggtcag cctgtcaggc accgaacccc atgacctgtt
      841 taacatattc cagagggaaa tctttgccag tccataacgt gaactgttca gccccttgcc
      901 acaacaacat gccgtatcca tcaatcgttt tgcaaccagc ttgttgcgcc tgctgcaata
      961 acttcgtcat atgcgggtta tacacgcatt cagtgaccag cagtcccgga tgtaacagac
     1021 tgatatcatt aactaatgat tcattctcaa ggggtttcat acccactttt gtgccattgg
     1081 ttaaaatgtc ggcggaagcc agggcttcag caaaggcttg ctgatcggcg agatcggtaa
     1141 ccgtgacgac acaatcggtg ttttcgttaa cccgctgcgc gaaggcgagg gctttatcga
     1201 agaactcatc ccgacggtta aagagtttaa tttcttttaa accttcaatt gccccctgcg
     1261 cgccaatcgc cgttgaggca cctccggccc ctaacagcac cattgttttg cctttgatat
     1321 caaaaccgct ctctttaatg gcgcgaatat ggcccgtgcc gtcggtgtta tagccgcgca
     1381 ggtagccatc atcattaacg atggtgttga tggcacccac cagtttggct gctggcgtta
     1441 attcatcaac atattcacac gccagttgtt tgttcggcat tgatatacca gttccgcgca
     1501 ttttgagggc ttttaatcct tcaattgctc cgggaaaact atcgttatcc acttcaaagg
     1561 ccatataggt aaatggcaat cccgcttttt ctaaggcttt attctgcatt tcaggcgata
     1621 aactgtggcg gataggatag gccatcagcc caatcaattc gtattttgcg gtaacatcca
     1681 taattaactc ctttaacctc tatgcttaat tgtgtttaac cgggtgctaa atttacgctc
     1741 gccaaaccgc acatcattct caggaataat aaaaacgcgg taatagcgga taaaaacaat
     1801 aattgcggta atcagggcca gcagcgcgaa agtaaaatcg agaacaatga tatattgaag
     1861 gccgatgttc gacagataac cggtaatcag cggaataaca aagttagcca gtccacccat
     1921 catcatataa atactggtga ctttggcttt gctttttggg aaaaactctg acatgaccga
     1981 aacaccgagc tgtaaaatgc cgccagctgc tgagaaaccg ataacaaagg cgccggcatt
     2041 acacaccagt ggagaagggt acaggtagat aatggctgct gttattgttg ccagtgcaga
     2101 gttaaataca ttagcccaga tgggccggac catttttttc agtagtgcgg caaaaataaa
     2161 gacacagacc aacgagccca tactgtaata agagatggtt tttaatgcct cagcttctga
     2221 cataccagca aaagccatcg catatttggg catccacacc acaatcacat aaaaggtcga
     2281 gaatgcggct acaccgaaca gtaccgatga aacaccttcc agccagacta acggtttgct
     2341 gttcatttgc ggtaattcat tagctacgct ggcgtccacc aactggctgg ggaatttgct
     2401 tttcaacagc atcagcgtga tcagtacaaa cagaataccc ggaataatca gcccatagcc
     2461 gtaccagata ttattgagca acatatagct caccagcatt gggtagaaca tttgcccaaa
     2521 tgacaccatc gctttaacca gtatgaccgc cgaaccagag gctttcggaa agcattccat
     2581 gagcgcgggg tagccacccg tatccagcgc tgagttagcg atacctacgc acactgccag
     2641 accgtaggcg agagttaaat tcgggcaagc gggaatacca aagaagaata gcagatacat
     2701 tattactgcc attaatatca ccgcccgacg accaaactta tcggagatca caccgaagaa
     2761 taaaatactg atcaatcgcc ccaaaccgat accggaaatt aagtaggcaa tgcccgcgtt
     2821 gtcagtggaa aacttttctg ccagagatga catgttttgg gcaagcgtaa taacactaat
     2881 gccgtgcagg aaatagctga agtaaataca aagaacagcc aggataaatg gcgtgctgaa
     2941 agccttattt tgagacattt tcacagctcc ttgctgaaag ataatttcgt taccgtacgt
     3001 attgttctcc gacggtttac ataattacta tactgaagat gattttccct gggtacatgg
     3061 cataaagttt atgtttccag gaatattgca tgtcatagtt tgtttttgat cgcgattttc
     3121 cttagttagc gcagaaagag ttatttatcc gtaagatctt gcatgacctg aaaagaatat
     3181 tgcattaccc acccggagcg acattttcct ttagcgagtg atgccgcgtt tttttgcggc
     3241 tgcgtaagcc gataaacagt gccagtaaaa agccgatggc agcgatggcg gtatcgaacc
     3301 acataatatc ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg
     3361 tcgcaatact gcccgcactg taatagatcc ctgtagcttt acctttagcg taggggaaac
     3421 gttcagccat taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga
     3481 caaaagcaaa tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa
     3541 taaatgagat aaaggtgtac agcatcagta atgttgttgg gcgaacggta tttcgaatga
     3601 gtggagcggt aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga
     3661 tcgacatagt gtatgacatg cctgcaacaa attgtccgta ctgtgacagc cactggctaa
     3721 ccagataaaa cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa
     3781 ttgaacagcg atgttccgta gagcgggtgg ttttctttat gacaggtaag cgacgacccg
     3841 gatggggtgg gaacgtacaa cggtataaaa acagagcgtt aataagcata atgcctgcag
     3901 caatcataaa ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg
     3961 gtaataaaaa ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg
     4021 agcgtggaaa agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca
     4081 ttcccgccag aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgccaa
     4141 aaaagaaggc catatagcaa cacatcccga gcatgataaa agggcggcga ccaaagcgat
     4201 cggataataa tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa
     4261 ctatcgagac acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc
     4321 tcatcaaaat gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag
     4381 ggtaataggg atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg
     4441 ttgtgtcagt atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt
     4501 agcaaagccg ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc
     4561 agtcaataaa attgccatca gggtagactc gttgaaatgc ggttaagtcg gggaaaaaac
     4621 gcatggtgtt gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt
     4681 ttctttgctg acgcatagcc aacagttgct cgactacacg atcaggaatg gcacacttgc
     4741 cattttccca ttgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt
     4801 catcaatagt catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt
     4861 aatggctgca tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg
     4921 aagagagtca cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag
     4981 cggaaaaatg ttaaaaaaaa gcccatcgtg ggagatgggc aaagactaca cacagcaatt
     5041 cgttgtttca ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga
     5101 gctaatagta ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac
     5161 tgtaaagaat ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga
     5221 agagcaagca aatatcaatt agcggtggtt aacaatcagg cggcagagcc gcctgatgag
     5281 aaattactta ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc
     5341 cgtcggcggc gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag
     5401 aaccggacca ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga
     5461 gaggatcagg atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt
     5521 atccagcgtg ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc
     5581 ggtccagtag agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag
     5641 gcaggagagg atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag
     5701 gccgataccg ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc
     5761 gcggatagcc tgtgccgcca gcagtacggc ggcatctccg cgaacgcctg ccagacgggt
     5821 tgcaaaatgg cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa
     5881 gagaagcatc agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa
     5941 ccaggtggtg gtggtgccaa tataagggcg gactttcgcc atgatcgccg tcccccccat
     6001 atccagcaag ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag
     6061 ccacgccaga tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct
     6121 gccgtcgacg atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa
     6181 cagcgtcatc accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa
     6241 caataccggc caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg
     6301 ttgaacaatc cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat
     6361 ttgtgcgaca tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc
     6421 gtcaaatcgt cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc
     6481 agcggttttt aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt
     6541 ggtacaacaa ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc
     6601 tgcggctacg ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct
     6661 tgccgccttc ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta
     6721 ttgcagccgc ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa
     6781 tataatgatt ccacagattt cccaggcacc cggcgtcgtt caactggtgc ttaatttttt
     6841 gcaagagctg gagcaacaag gttttaccgg cgatacggcg acaagttatg ccgatcgtct
     6901 gacaatgtcg accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg
     6961 ttcaaccgca gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct
     7021 gatctttacc ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat
     7081 tattgttgat atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg
     7141 ggtgcgcgtt gaggccgggg tgataaaaga tcaactcaat cagtacctga aaccgttcgg
     7201 ctactttttt gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa
     7261 taccgatgca tccggtcagg gatcgctggt ctatggcaaa acgtcggatc acgtacttgg
     7321 cgtacgcgcg gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgaact
     7381 ggcggaaacg ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca
     7441 acgttgccgt cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct
     7501 taccggttac gatctgcgtt atgtctttaa cgatgagatg accgagttcg acctgacgcg
     7561 cattctgacg ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac
     7621 gcccttgcct aaagtgcgcc gtctggtgaa cgtcaaatat gactcttttg actccgcgct
     7681 gcgtaacgcg ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa
     7741 agtgctgaat ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga
     7801 tgtgcctgac aaagagatgc tcgggctgaa cattgtggag tttgctggtg atgatgaggc
     7861 gctgattgac gagcgagtta atgctctctg tgtgcggctt gatgagctga ttgccagcca
     7921 acaggcaggt gtgattggct ggcaggtgtg ccgcgagctg gcgggtgttg aacgtatcta
     7981 tgcgatgcgt aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat
     8041 tccgtttgct gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt
     8101 tcgcgcgctg ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg
     8161 tgtcttgcac gtccgtccag cgctggatat gtgcgatcct caacaagaga ttttgatgaa
     8221 gcaaatctct gatgacgtag tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga
     8281 gcacggcaaa ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc
     8341 agaactgcgc aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat
     8401 ttgcccgcca gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac
     8461 attcgatcgg cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa
     8521 cggcaacggt ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat
     8581 cacccagaac cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg
     8641 tttgttggcg gatcgcggcg ttgatccact caaactggaa caagaactgc ctgaatccgg
     8701 cgtcagtttg cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga
     8761 atatgacttc tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcgtg
     8821 ttcgacccag tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct
     8881 ctatcacacc cgttatttac gcccgctgcg cgaccacctc gtcgctacgg tcgagagcta
     8941 cgcgccgctg atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt
     9001 gcgcaaactc tcggaaaaac atatcggcat ggttgatttg ccgctgctgt cggtcccctc
     9061 gctacaacaa caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaatc
     9121 tctcaatgca gagcagaaag cgcgcacagt gttggtggtg caggacccct ttaccagcta
     9181 ttacgatgcg caagtggtgg cggattttgt ccgtctggtc gaaaaattag gtttccagcc
     9241 tgtgttactg ccattttcac caaatggcaa agcccagcat atcaaaggct ttcttaatcg
     9301 ttttgcgaag acggcgaaaa agacggcgga tttcctcaac cgtatggcga agctgggtat
     9361 gccaatggta ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc
     9421 actgggcgag gaacgtggcg agtttaacgt cttactggcg aatgaatggc tggcaagcgc
     9481 acttgagtca cagccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg
     9541 taccgaagtt accgccttgc cgggtgcgcc agcacaatgg gccgcgatat ttgcccgttt
     9601 tggcgcgaaa ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca
     9661 tgaagcgaaa aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat
     9721 gcagcgactg ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa
     9781 acgggttgaa ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg
     9841 atatggaaac ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg
     9901 gggttcctgg atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca
     9961 gtagactcgc ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg
    10021 gccgaaagta tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt
    10081 ggtctggaaa tcaatgctaa ccacgtccgc tcggcacgag aagggcgggt gcgcggcgta
    10141 tgcaaaccgt tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag
    10201 aaagggcgtt tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag
    10261 aaaagtgtga cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg
    10321 caggtcttgt ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc
    10381 attgaatttt tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg
    10441 aaacaactag aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa
    10501 gaaatctact ttgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg
    10561 ccaaatcgac ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct
    10621 accgcctgat attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag
    10681 ctttatgaga ttattcttat cgccccttca agagctaagc cactgagagt gccggagata
    10741 agcgccggat ggggtagaaa cccttaagcc tgtgttgcac agacttaagg gttttcttat
    10801 ttctgaacgc tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc
    10861 tttttcatca cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct
    10921 aattcttagc gtgcctgtta acccacacat cagggtctat gcttattaaa agcaggcaaa
    10981 aagcatgttt tacggtaaag cccctgcgtt tgctgggttg tactgataat cattatcact
    11041 aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca tgcattcagg
    11101 aacctttaac ccacaagatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat
    11161 acacatccgt gagctggtgg caaagcagcc gggtatggtc ggcgtgcgct taggcgtgaa
    11221 gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga
    11281 cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaag cgatgccgtt
    11341 tattgatggc acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca
    11401 caaccctaaa gcccagaatg aatgtggctg tggcgaaagc tttggggtat aggcggtact
    11461 atgtctcgta atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaat
    11521 tataaagaag gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa
    11581 gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct gaagtttcgt
    11641 ctaaacgcct atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac
    11701 gacaagctga attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac
    11761 gacacttgcg cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt
    11821 aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg
    11881 gcggtggatg ccattttcga ctcagtttcg gttgccacta cttatcgcga aaaactggcg
    11941 gagcagggaa ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg
    12001 cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact tctttgccgc gcttaatgcg
    12061 gcggtagcct ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa
    12121 ctttccacct attttcgcat taacgcggaa aaaaccgggc agtttgagcg caccattctg
    12181 gtggccgacg aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac
    12241 agctatcagt tacacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa
    12301 tattccacgg tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc
    12361 gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa
    12421 accgggtccg cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt
    12481 ggtgagtttt actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag
    12541 atgatccaca tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga
    12601 catagtcaga acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc
    12661 aatttcactc agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg
    12721 tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt
    12781 ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg
    12841 atgattgtta acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt
    12901 gaagcacaaa aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg
    12961 ttaagtatta aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc
    13021 ctcgccgttc gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt
    13081 accttatcgg caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag
    13141 ttcaaaggca aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt
    13201 atggccttcc agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg
    13261 gcacttaatg cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag
    13321 gatttgatgg aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg
    13381 gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg
    13441 ctggaaccgg agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta
    13501 aaagtggtcg ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt
    13561 acgcactacc aacgcattct cgactacatc aagcctgatt acgtccatgt gctatatcag
    13621 ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat
    13681 ggctggctta ccgaacagca gtaacgcgct gcaacagtgg catcacttgt ttgaagctga
    13741 agggacaaaa cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact
    13801 gccgacacgt aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagcca
    13861 gtttgtcagc attgcgggag agatatcccc acagcagcga gatgccttag cgttaacgtt
    13921 agacgctgtg cggctggtat ttgtcgatgg acgttacgtg tcagcactga gcgatgcgac
    13981 tgaaggcagc gggtatgaag tgagcattaa cgacgaccgt cagggggtac ccgacgctat
    14041 tcaggcggaa gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc
    14101 cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta atgcatatta cccagggcgt
    14161 ggcaggtgaa gaggtcaaca ctgcccatta ccgacatcat ctggatctgg cggaaggtgc
    14221 cgaagcaacg gtgatcgaac attttgtcag ccttaatgat gctcgtcact tcaccggcgc
    14281 acggttcact atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga
    14341 aaacccggtc agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc
    14401 atttagccat agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact
    14461 caatggcgaa aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt
    14521 gtgtgatacc cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca
    14581 caaaactatc gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca
    14641 gcacgccatc aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc
    14701 agaagtggat acgaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg
    14761 cgcgacggtg gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa
    14821 tcagcaggat gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact
    14881 gcgtgatgag gggcttaaac agcaggtgct ggcccgaatc ggtcaacgac tgccaggagg
    14941 tgcaagatga ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag
    15001 gtaaacggtt tgccgctggc ttatctcgac agcgccgcca gtgcgcagaa accaagccag
    15061 gtgattgact ccgaagccga gttttatcgt cacggctacg cggcggtgca tcgcggtatt
    15121 cataccttaa gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg
    15181 tttattaatg cccgttcggc ggaagagctg gtgttcgtcc gcggcacgac ggaagggatc
    15241 aatctggtcg ccaatagttg gggcaacagc aacgtgcggg cgggcgataa catcatcatc
    15301 agtcagatgg agcaccacgc taacattgtt ccctggcaga tgctttgcgc acgcgttggc
    15361 gcagagctgc gtgtgatccc gctcaatccc gacggtacgc tgcaactgga gacgctacct
    15421 aatctgttcg atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc
    15481 acagaaaatc ccctggcgga aatgatcacg cttgcgcatc agcatggcgc aaaagtgctg
    15541 gtggatggcg ctcaggcggt gatgcatcat ccggtggacg ttcaggcgct ggattgcgat
    15601 ttttacgtat tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg
    15661 aaagaagcct tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc
    15721 gtcagcctga gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca
    15781 cccaataccg ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg
    15841 cttaataaca tagccgagta tgaacagaat ctgatgcact acgcgctatc acagctggaa
    15901 tctgtaccgg atctcactct ctatggcccg cggaacaggc ttggcgttat tgcttttaat
    15961 cttggtaaac accacgccta tgatgttggc agttttctcg ataactacgg cattgctgtg
    16021 cgtaccggac atcactgcgc tatgccatta atggcctatt acaacgtccc tgctatgtgt
    16081 cgggcgtcgc tggccatgta taacacccat gaagaagtgg atcgtctggt gaccggactg
    16141 caacgtattc accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa
    16201 gaaaaattgc tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt
    16261 attgagctgg gccagcgtct gccagaatta cgcgccgaag accgaagccc acaaaatagc
    16321 attcagggct gtcagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt
    16381 gaattgcagg gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt
    16441 ctctacgatc agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa
    16501 aaaatggcgc tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt
    16561 cgcgcaattc gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg
    16621 ttggctcaca ttgccggatg gtcaatgttt acactgacct gcacaggagt gttttggttt
    16681 caggtgaaca taagcgccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt
    16741 tacaatgaaa cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca
    16801 ggctgcctgg gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa
    16861 tcaaacgtat acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga
    16921 tactgcggca atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg
    16981 tacgacgata actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat
    17041 cgttaacctt gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt
    17101 ttatccaata ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg
    17161 gcagaaaatc cctaacccaa cctggacgcc tacggcaggc attcgtcaac gttcgctgga
    17221 gcgtggcatt aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta
    17281 cgcactgcgc ctcgcgcatg gtaatggcga atacctcatt catggtacca gtgcgccgga
    17341 cagcgtcggt ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc
    17401 cttgttctcc agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata
    17461 ttccgtggaa cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga
    17521 acagcaaaac gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga
    17581 caataaggct gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc
    17641 ggtttcggtg agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc
    17701 gcagaatggt gaaccagagc aagggaatat gttacgcgcg acgcagtagc ggtaaacggc
    17761 agacaaaaaa aatggcgcac aatgtgcgcc atttttcact tcacaggtac tattacttgc
    17821 ggtatttagt agccatgttg tccagacgct ggttagcacg agctgcgtca tctttagcag
    17881 cctgaacgtc ggaacgcatt gcgttcacgt cgttgctcag ctggtcaact ttagcgttca
    17941 gagtctgaac gtcagaagac agctgatcga ttttagcgtt gctggagcaa cctgccagca
    18001 gagtagaacc caggattacc gcgcccagta ccagtttagt agctttcatt attaataccc
    18061 tctagattga gttaatctcc atgtagcgtt acaagtatta cacaaagttt tttatgttga
    18121 gaatattttt ttgatgggaa ggcacttatt tttgatcgtt cgctcaaaga agcatcgaac
    18181 gctgggtttt agataaaaaa attgagagaa aacaggatgc ttccatcgga ttcatcttag
    18241 ataaagtgag attatatagt gaaatccgat ttgatttttt attgcttctg gttatcgatt
    18301 aaataaaaaa agcgcccatc agggcgcttc gatatacaaa ttaattcaca aaagcaatat
    18361 tacaggacgt gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc accagaaacc
    18421 ataactacaa cgtcaccttt gtgtgccaga ccgctctgca gagctagttc tttacccaga
    18481 cggtagaaat catcagtaga agtgatctct ttaaccagct gcggcacaac gcctttgctc
    18541 agtaccaact gatgagccgt tttttcgttg gtggtcagtg ccaggatggt ggcatccggg
    18601 aagtatttac gtactgcgcg agcagattta ccgccctgag tagcaaccac gatcagcgga
    18661 gcatccagtt tttcagcagt ttcaacggca ccacggcata ccgcttcggt aatgcgcagt
    18721 ttacggttgt cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt acgttcgcaa
    18781 atggtcgcca tgatagaaac cgcttccagc gggtatttac cttttgcgga ttcaccagac
    18841 agcatcactg catcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc ttctgcgcga
    18901 gtcgggcgtg ggtttttgat catggaatcc agcatctggg tcgcagtgat aacgacttta
    18961 cgtgcacgga tacatttttc gatcatcatc ttctgggcga agataacttc ttctaccggg
    19021 atttctacac ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc gaggatttcg
    19081 tcgaagttgt tgaggccttc ctggttttcg attttggaga tgatgtggat gttttcgccg
    19141 ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag aacgcttacg aataaaggaa
    19201 gcagcaacaa agtctacgcc ttgttcgcaa ccaaagatca ggtcctgttt gtctttttca
    19261 gccagtgctg gcagagcaat ggaaacgcca ggcaggttca cacctttgtt ttcgcccagg
    19321 tcaccgttgt tcagcacttt acagataact ttgttacctt caatggcggt aacttccata
    19381 ccgatcagac catcgtcaac cagtacggtg ttgccaactg acaggtcagt agtgaaacct
    19441 tcatacgtta ccgcaaccat ttcgctgttg ccgataacag atttatcagt ggtgaaagta
    19501 aaggtctgac cagctttcag agaaacgtcg ttaccgcctt ccagtttcat ggtgcggatt
    19561 tccggacctt tggtatcaag caggatagcg gcggttttac cagttttgct catcacgttg
    19621 cgcagattct gaatgcgctg accgtgttct gcatagtcac catgagagaa gttcagacgc
    19681 ataacgttca tgccagcgtc cagcatttta gctaacatct cttcagattc ggttttcggt
    19741 ccgatggtgc aaacaatttt ggtctttttc atgacagtct tagtctttaa gttgagaagg
    19801 atgggagaaa cttgctttct gggcgcactg ccggaaagcg aatccggtat tacgaaagtg
    19861 gtggtgcctc gctctaagga taggtgacat cgaaaaagcg tgcagaggaa tgtgtgcttg
    19921 tgtctcagcc caacgtgagt gcctgttttt gagtcgttga gttctacagt ccaaagtgct
    19981 gaaaccattc aagagtcaat tggcgcgcat tatacgctga aattaatcca aaaggaaaac
    20041 aaaaacagcg tttcaaaaaa tatacaactt gttagacaat aaattgttat caaagattta
    20101 acgttccagg gaaaaggtta cgcaaaaact tcgctgtttg cgca
//
LOCUS       DAPHDX010000078        19858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPHDX010000078 DAPHDX010000000
VERSION     DAPHDX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.078"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(447..1694)
                     /locus_tag="RH058_002850"
     CDS             complement(447..1694)
                     /locus_tag="RH058_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDV3138415.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLIPGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1766..2680)
                     /locus_tag="RH058_002851"
     CDS             complement(1766..2680)
                     /locus_tag="RH058_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV3138416.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            2896..4329
                     /gene="cscA"
                     /locus_tag="RH058_002852"
     CDS             2896..4329
                     /gene="cscA"
                     /locus_tag="RH058_002852"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV3138417.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(4337..5395)
                     /locus_tag="RH058_002853"
     CDS             complement(4337..5395)
                     /locus_tag="RH058_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3138418.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            5575..5793
                     /locus_tag="RH058_002854"
                     /pseudo
     CDS             5575..5793
                     /locus_tag="RH058_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            5811..7139
                     /gene="dsdA"
                     /locus_tag="RH058_002855"
     CDS             5811..7139
                     /gene="dsdA"
                     /locus_tag="RH058_002855"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV3138419.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(7247..8785)
                     /gene="emrY"
                     /locus_tag="RH058_002856"
     CDS             complement(7247..8785)
                     /gene="emrY"
                     /locus_tag="RH058_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV3138420.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(8785..9948)
                     /gene="emrK"
                     /locus_tag="RH058_002857"
     CDS             complement(8785..9948)
                     /gene="emrK"
                     /locus_tag="RH058_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV3138421.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALIMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            10364..10978
                     /gene="evgA"
                     /locus_tag="RH058_002858"
     CDS             10364..10978
                     /gene="evgA"
                     /locus_tag="RH058_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV3138422.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            10983..14576
                     /gene="evgS"
                     /locus_tag="RH058_002859"
     CDS             10983..14576
                     /gene="evgS"
                     /locus_tag="RH058_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV3138423.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYTEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(14632..15777)
                     /gene="yfdE"
                     /locus_tag="RH058_002860"
     CDS             complement(14632..15777)
                     /gene="yfdE"
                     /locus_tag="RH058_002860"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV3138424.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWEMLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(15851..16795)
                     /gene="yfdV"
                     /locus_tag="RH058_002861"
     CDS             complement(15851..16795)
                     /gene="yfdV"
                     /locus_tag="RH058_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV3138425.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(16865..18559)
                     /gene="oxc"
                     /locus_tag="RH058_002862"
     CDS             complement(16865..18559)
                     /gene="oxc"
                     /locus_tag="RH058_002862"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV3138426.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(18613..>19858)
                     /gene="frc"
                     /locus_tag="RH058_002863"
     CDS             complement(18613..>19858)
                     /gene="frc"
                     /locus_tag="RH058_002863"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV3138427.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         5752 a         4657 c         4272 g         5177 t
ORIGIN      
        1 caccagatac ccttcaaacg aaatctacct tcaccccgta aaagatgggt ttggcagcac
       61 acttgcctta tatctactca tttttactgc aacaggttga aatctcagca ctgtcagaaa
      121 gcgctgatga ctaaacagcc ctgagccggg cgatgtaacc atcacacaga atcctgatag
      181 cgaaatatgg cgtgactcga tacttcactc cgcaatgcat tccttgatga attcgcagga
      241 ccgtgataca cgggacaggt cactgaatga cgacaatgtc ctggaaatca gcgaaccgcg
      301 catctgaagt acatttgagc gactgtacca gaacatgaat gaggcgtttg gattaggcga
      361 ttattagcag ggctaagcat tttactatta ttattttccg gttgagggat atagagctat
      421 cgacaacaac cggaaaaagt ttacgtctat attgctgaag gtacaggcgt ttccataact
      481 atttgctcgc gttttttact caagaagaaa atgccaaata gcaacatcag gcagacaata
      541 cccgaaattg cgaagaaaac tgtctggtag cctgcgtggt caaagagtat cccagtcggc
      601 gttgaaagca gcacaatccc aagcgaactg gcaatttgaa aaccaatcag aaagatcgtc
      661 gacgacaggc gcttatcaaa gtttgccacg ctgtatttga agacggatat gacacaaagt
      721 ggaacctcaa tggcatgtaa caacttcact aatgaaataa tccaggggtt aacgaacagc
      781 gcgcaggaaa ggatacgcaa cgccataatc acaactccga taagtaatgc attttttggc
      841 cctacccgat tcacaaagaa aggaataatc gccatgcaca gcgcttcgag taccacctgg
      901 aatgagttga gataaccata caggcgcgtt cctacatcgt gtgattcgaa taaacctgca
      961 taaaagacag gaaaaagttg ttgatcaaaa atgttataga aagaccacgt ccccacaata
     1021 aatatgacga aaacccagaa gtttcgatcc ttgaaaactg cgataaaatc ctcttttttt
     1081 acccctcccg catctgccgc tacgcactgg tgatccttat ctttaaaacg catgttgatc
     1141 atcataaata cagcgccaaa tagcgagacc aaccagaagt tgatatgggg actgatacta
     1201 aaaaatatgc cggcaaagaa cgcgccaata gcatagccaa aagatcccca ggcgcgcgct
     1261 gttccatatt cgaaatgaaa atttcgcgcc attttttcgg tgaagctatc aagcaaaccg
     1321 catcccgcca gataccccaa gccaaaaaat agcgcccccg gaattagacc tacagaaaaa
     1381 ttgctttgca gtaacggttc ataaacgtaa atcataaacg gtccggtcaa gaccaggatg
     1441 aaactcatac accagatgag cggtttcttc agaccgagtt tatcctgaac gatgccgtag
     1501 aacatcataa atagaatgct ggtaaactgg ttgaccgaat aaagtgtacc taattccgtc
     1561 cctgtcaacc ctagatgtcc tttcagccaa atagcgtata acgaccacca cagcgaccag
     1621 gaaataaaaa agagaaatga gtaactggat gcaaaacgat agtacgcatt tctgaatgga
     1681 atattcagtg ccataattac ctgcctgtcg ttaaaaaatt cacgtcctat ttagagataa
     1741 gagcgacttc gccgtttact tctcactatt ccagttcttg tcgacatggc agcgctgtca
     1801 ttgccccttt cgccgttact gcaagcgctc cgcaacgttg agcgagatcg ataattcgtc
     1861 gcatttctct ctcatctgta gataatcccg tagaggacag acctgtgagt aacccggcaa
     1921 cgaacgcatc tcccgccccc gtgctatcga cacaattcac agacattcca gcaaaatggt
     1981 gaacttgtcc tcgataacag accaccacac cttctgcacc tttagtcacc aacagcatgg
     2041 cgatctcata ctcttttgcc agggcgcata tatcccgatc gttctgtgtt tttccactga
     2101 taagtcgcca ttcttcttcc gagagcttga cgacatccgc cagttgtagc gcctgccgca
     2161 aacacaagcg gagcaaatgc tcgtcttgcc atagatcttc acgaatatta ggatcgaagc
     2221 tgacaaaacc tccggcatgc cggatcgccg tcatcgcagt aaatgcgctg gtacgcgaag
     2281 gctcggcaga caacgcaatt gaacagagat gtaaccattc gccatgtcgc cagcagggca
     2341 agtctgtcgt ctctaaaaaa agatcggcac tggggcggac cataaacgta aatgaacgtt
     2401 ccccttgatc gttcagatcg acaagcaccg tggatgtccg gtgccattca tcttgcttca
     2461 gatacgtgat atcgactccc tcagttagca gcgttctttg cattaacgca ccaaaaggat
     2521 catcccccac ccgacctata aacccacttg ttccgcctaa tctggcgatt cccaccgcaa
     2581 cgttagctgg cgcgccgcca ggacaaggca gtaggcgccc gtctgattct ggcaagagat
     2641 ctacgaccgc atcccctaaa acccatactt tggctgacat ttttttccct taaattcatc
     2701 tgagttacgc atagtgataa acctcttttt cgcaaaatcg tcatggattt actaaaacat
     2761 gcatattcga tcacaaaacg tcatagttaa cgttaacatt tgtgatattc atcgcattta
     2821 tgaaagtaag ggactttatt tttataaaag ttaacgttaa caattcacca aatttgctta
     2881 accaggatga ttaaaatgac gcaatctcga ttgcatgcgg cgcaaaacgc cctagcaaaa
     2941 cttcatgagc accggggtaa cactttctat ccccattttc acctcgcgcc tcctgccggg
     3001 tggatgaacg atccaaacgg cctgatctgg tttaacgatc gttatcacgc gttttatcaa
     3061 catcatccga tgagcgaaca ctgggggcca atgcactggg gacatgccac cagcgacgat
     3121 atgatccact ggcagcatga gcctattgcg ttagcgccag gagacgataa tgacaaagac
     3181 gggtgttttt caggtagtgc tgtcgatgac aatggtgtcc tctcacttat ctacaccgga
     3241 cacgtctggc tcgatggtgc aggtaatgac gatgcaattc gcgaagtaca atgtctggct
     3301 accagtcggg atggtattca tttcgagaaa cagggtgtga tcctcactcc accagaagga
     3361 atcatgcact tccgcgatcc taaagtgtgg cgtgaagccg acacatggtg gatggtagtc
     3421 ggggcgaaag atccaggcaa cacggggcag atcctgcttt atcgcggcag ttcgttgcgt
     3481 gaatggacct tcgatcgcgt actggcccac gctgatgcgg gtgaaagcta tatgtgggaa
     3541 tgtccggact ttttcagcct tggcgatcag cattatctga tgttttcccc gcagggaatg
     3601 aatgccgagg gatacagtta ccgaaatcgc tttcaaagtg gcgtaatacc cggaatgtgg
     3661 tcgccaggac gactttttgc acaatccggg cattttactg aacttgataa cgggcatgac
     3721 ttttatgcac cacaaagctt tttagcgaag gatggtcggc gtattgttat cggctggatg
     3781 gatatgtggg aatcgccaat gccctcaaaa cgtgaaggat gggcaggctg catgacgctg
     3841 gcgcgcgagc tatcagagag caatggcaaa cttctacaac gcccggttca cgaagctgag
     3901 tcgttacgcc agcagcatca atctgtctct ccccgcacaa tcagcaataa atatgttttg
     3961 caggaaaacg cgcaagcagt tgagattcag ttgcagtggg cgctgaagaa cagtgatgcc
     4021 gaacattacg gattacagct cggcactgga atgcggctgt atattgataa ccaatctgag
     4081 cgacttgttt tgtggcggta ttacccacac gagaatttag acggctaccg tagtattccc
     4141 ctcccgcagc gtgacacgct cgccctaagg atatttatcg atacatcatc cgtggaagta
     4201 tttattaacg acggggaagc ggtgatgagt agtcgaatct atccgcagcc agaagaacgg
     4261 gaactgtcgc tttatgcctc ccacggagtg gctgtgctgc aacatggagc actctggcta
     4321 ctgggttaac ataatatcag gtggaacaac ggatcaacag cgggcaaggg atccgcgtca
     4381 ctcttccccc ttcacgacct tcaataatat gcaatgcagc ttcccgcccg ataatgtcat
     4441 gtggaagctg aattgtggtc agcggcggta aaaacagatg cccgacgcca accagattat
     4501 caaagcccat tacggcgaca tcctgcggga tacgtacccc cttcgccagg agaacctgat
     4561 aagcgacaaa ggctgcgcga tcgttaccac atatcagaac atcaaaatct ggtttgcccg
     4621 gtttgaagtg ggcattgagt aaacttgcga gatcggtgta gtgatcatca cctgttgcca
     4681 tgtgaaattg tttcacctca gccagatctc gtccagcatc acgccaggcc tgctcaaacc
     4741 cctgccgacg ataccctgtt gccaacgcac tttccggtag ccagaagcat aacggttgac
     4801 gatagcccgc cgcgagcaaa tgctgtgttg attcatattg tgcagtgtaa tcatcaggga
     4861 tataactggg taacgctggg tcatccgcca cacagttcgc caatacaata ttttcaccat
     4921 acagagactc aggcagcgtg atatgtcgca gccccattgt agtatagata atgccatccg
     4981 gacggtgggc aagcagctga cgtgccgcgc gggcagcgtc atcttcagaa aaaatattga
     5041 ttaaaaaact attccagccg aactcgctgg cggtttgctc aatagcaagc agaatatcaa
     5101 cagagaaagg agtggtagcc gtgtcctgcg ccagcacggc gagagtcgac ggcttacgtc
     5161 cttgagcgcg catcttacgg gcggaaagat caggaacata attcagggtc tggattgcct
     5221 gcaatacgcg gtcacgcgtt gcaggacgca cagattctgc attatgcatc acccgggaga
     5281 ctgtcatcat cgacactccc gccaggcgtg cgacatcctt taatgaagcc atacccaagc
     5341 cgtttgccgt aaaacgggca ctgtagcaga aacagacgtc actggcgaga tccaacgata
     5401 ttctcaacgc cctatcaccc aacacggcaa tacaataaaa aataacaata attgccggat
     5461 aattgtactc aattccgcct ctgttctcgc caacgagtaa ccatataggc aagtaagtaa
     5521 aacatgccaa ctggcatcgc caacctggag aagaaaatat aaatatggat cattgtgctg
     5581 ccgctgtatc ccgacatcag cccggaaatt attgcgattg ctatcggttc aggtgcaatt
     5641 ggctgcacga tcgttacgga ctcgcttttc tggctagtga agcaatattg cggcgctacg
     5701 ctcaatgaaa catttaaata ctatacgaca gcgacattta tcgcttcagt cgtcgctctg
     5761 gcgggcacat tcctgctgtc atttatcatc taagcgcaaa gagacgtact atggaaaacg
     5821 ctaaaatgaa ctcgctcatc gcccagtatc cgttggtaaa ggatctggtt gctcttaaag
     5881 aaaccacctg gtttaatcct ggcacgacct cattggctga aggtttacct tatgttggcc
     5941 tgaccgaaca ggatgttcag gacgcccatg cgcgcttatc ccgttttgca ccctatctgg
     6001 caaaagcatt tcctgaaact gctgccactg gggggattat tgaatcagaa ctggttgcca
     6061 ttccagctat gcaaaaacgg ctggaaaaag aatatcagca accgatcagc gggcaactgt
     6121 tactgaaaaa agatagccat ttgcccattt ccggctccat aaaagcacgc ggcgggattt
     6181 atgaagtcct ggcacacgca gaaaaactgg ctctggaagc ggggttgctg acgcttgatg
     6241 atgactacag caaactgctt tctccggagt ttaaacagtt ctttagccaa tacagcattg
     6301 ctgtgggctc aaccggaaat ctggggttat caatcggcat tatgagcgcc cgcattggct
     6361 ttaaggtgac agttcatatg tctgctgatg cccgggcatg gaaaaaagcg aaactgcgca
     6421 gccatggcgt tacggtcgtg gaatatgagc aagattatgg tgttgccgtc gaggaaggac
     6481 gtaaagcagc gcagtctgac ccgaactgtt tctttattga tgacgaaaat tcccgcacgt
     6541 tgttccttgg gtattccgtc gctggccagc gtcttaaagc gcaatttgcc cagcaaggcc
     6601 gtatcgtcga tgctgataac cctctgtttg tctatctgcc gtgtggtgtt ggcggtggtc
     6661 ctggtggcgt cgcattcggg cttaaactgg cgtttggcga tcatgttcac tgcttttttg
     6721 ccgaaccaac gcactcccct tgtatgttgt taggcgtcca tacaggatta cacgatcaga
     6781 tttctgttca ggatattggt atcgacaacc ttaccgcagc ggatggcctt gcagttggtc
     6841 gcgcatcagg ctttgtcggg cgggcaatgg agcgtctgct ggatggcttc tataccctta
     6901 gcgatcaaac catgtatgac atgcttggct ggctggcgca ggaagaaggt attcgtcttg
     6961 aaccttcggc actggcgggt atggccggac ctcagcgcgt gtgtgcatca gtaagttacc
     7021 aacagatgca cggtttcagc gcagaacaac tgcgtaatac cactcatctg gtgtgggcga
     7081 cgggaggtgg aatggtgccg gaagaagaga tgaatcaata tctggcaaaa ggccgttaat
     7141 aacgtttcaa cgcagcatcg caatcctttc cctgggtgag cgatgctgcc gatggcgcag
     7201 acttaagatc cccggtctta cccgctataa ccccctcctt ttatcatcac ccaacgcctt
     7261 tcgctgtaaa cggcggtttc gcaaaccaaa ccaaaaccgt aagtaagata aaagcaatag
     7321 ccgccatacg gaaaatctca tttgcagaaa tagaaagtga ctgctgggtt atttcattat
     7381 taatttcatt aagaactcct gaaagcgaac catagtattt atccataatt tgtgacgatg
     7441 aattaaacac ggggttaaat tgatcgatgg ttgctgtcaa ctgactatgg tgtaacgatt
     7501 cgcgtcgtcc ccacagcgtc attgtcaacg acgtaccaac tgatcctgac aaggtacgaa
     7561 aaaaattact catactcgag gcattggcaa atttattatc tggcaagcct gaaaacgaaa
     7621 tcgttgttaa gggtaaaaag aaacaggcaa cggcgaatcc ctggaaaaac tgtggcaaaa
     7681 tgatgcctgt aaaatcaatc gttggcataa atgtcacaga acgccagtaa tagcaaaccg
     7741 catacatcaa aaaactgaat gtcactaaca cccgcatgtc tattttattg ccataacgtc
     7801 ctatcaaagg tgaaatcaat agtggcatga tgccgatggg cgcataagca agcccggccc
     7861 atatcgcatt ataccccatc gtttcctgga gtaactgcgg cataaggacg atcgctccag
     7921 agtaaaataa atacgcgcag gtgatactca caataccaat ggtgaaatta cgagacttaa
     7981 acaaactgag atcaagaatc ggattctctg aggtcgactc ccaaatgact aaagagatca
     8041 gagaaataac tgatactact gttaatatta ttattgtact cgagttgaac caatccagat
     8101 cgcgcccttt atcaagcata atttgcaagc caccaacacc gagcactaac agggtcagtc
     8161 ctggtagatt catttttacc ggtgaagttt cagtttctct tcctttaagt aaggttaagc
     8221 ataatgtcag gacgataatc cccataggga cattgattaa aaatatccaa ccccagctaa
     8281 agttatcgca aatataaccg cccaatatcg gcccacatat cggagcgata atcacggtca
     8341 ttgaccataa tgccagagca aatgttcttt tttctggtgg ataattcctc aataataaac
     8401 tctgtgacag tggaattaac ggccccgcca ttaatccctg aacgactcta aaaaatatca
     8461 gcacatcaag attggtcgat aaactacaca ttaaggaaga cagcgaaaaa aaagtgactg
     8521 aaagtaaaaa taaccttaat tcgcctattc tttgtgccag cctgccggta acagggatcg
     8581 caatggcatt tgctacgcca aacgaggtga taacccaggt gccttcgtct gttgatgctc
     8641 ccagaaagcc agatattgtc ggtattgcga cgttagaaat agtggaatcc aacatttgca
     8701 taaatgtcgc taatgacaat gcaatagtga cgcaccataa cgtcccaccg gttaatggtg
     8761 ccggagttga tttagtgatt gccattaaag ttgtccatta tgcgaaataa tgttgctaat
     8821 ttctttttct atcggactgg tatcgataac taaagcctta ctggtataag ccggcatgga
     8881 ggtcacggtt gaagccagct caggcatctc ggcaatgtct tcgttcttcg tatcaatagt
     8941 tgctgtcatc gataaaccaa tacgcaaggg atgttccatg agttcttttg gatcaagaga
     9001 aacttcaacc ggtacacgct gaacgatttt gatccagttc cctgtcgcat tttgtgcagg
     9061 taataaggag aacgcattgc cggttcccat attgatccct gtcacccgac catgaaacac
     9121 aacattttca ccataaagat cgctgataat attgaccgat tgaccaatcc gtacatccgt
     9181 aagttgtgtt tctttaaagt tggcattaac ccacatttga cgtgccggta cgacagccat
     9241 taacgattgt ccggggctca ctgtttcgcc gacctgaaca cttctctggg caatatagcc
     9301 ggtaaccgga ctcttaatat ccgtacgttt aagcgccaac caggcttctt tagttgcatc
     9361 cgccgcttca acgacttgtg gctgacggtt taatggtgtg ttcattatta aagctttatt
     9421 cgctttataa gcctggatag cggcattcaa tgccgctttg ctacttatta acgtatcttt
     9481 ggtatgctcc agcgtttctt ttgaaataac cccctgcttc gctaacggca ctcgacggtt
     9541 ataatcttct aaagattgtt gatactgaat acgtgctgaa gcgacttcgg cactgtattg
     9601 tttatcctgt aagtatagtt tattcgtttg ccgaacaata tttgccagat tatttttagc
     9661 tttattgagt gcgatagtgg catcagtttt atccagtgaa actaaaatgt caccttgtcg
     9721 aacgtagttc gtatctttat gattaacgac agtgacacta cctgagactt gtgcagaaat
     9781 tggatctgca ttccccgtga cataggcgtc atctgtacta atcatgtctt ctaattccat
     9841 tgaccaatag gcataggcac ctgaaaacgc aataaataaa actaccgcca ataaagaaaa
     9901 gtattttctt ctgttagaat gttttttatt tgaattaatc tgttccacta ttatctctca
     9961 tttctcatag atgaaattta tgaattgaat gaaaagagca ttcattcttc atatcaaaca
    10021 cagtgcatgt gttgcacatt atcccaggaa cgttggaggc ctaatttaaa tgaggtgcaa
    10081 aaaaaacaac agaggactaa accgtggctt ttgcaataca attcttacgc ctgtaggatt
    10141 agtaagaaga cttatagtgc caacttgaaa ctataaatca tcggtacaat ccctgatttt
    10201 attgttgaca tttcatttat gccgactatt tatatggtat acttgtcgaa ttatcttaaa
    10261 ggaagctcag attttcttat ttttattgag aaaatgagat gacgccttat gtctgtatta
    10321 ctacagggag aagggagatg cttcattgca aagggaataa tctatgaacg caataattat
    10381 tgatgaccat cctcttgcta tcgcagcaat tcgtaattta ttgatcaaaa acgatattga
    10441 aatcttagcg gagttgactg aagggggaag cgccgttcag cgggtggaaa cacttaagcc
    10501 tgatatcgtc atcattgatg tcgatatccc cggagttaac ggtatccagg tgttagaaac
    10561 gctgaggaag cgccaatata gcggaattat tattattgtc tccgctaaaa atgaccattt
    10621 ttacgggaaa cattgtgctg atgctggcgc taatggattc gtgagtaaaa aagaaggcat
    10681 gaacaatatc attgcggcta ttgaagctgc aaaaaatggc tactgctatt tccccttctc
    10741 tctcaaccgg tttgttggaa gtttaacgtc cgaccagcaa aaactcgact ccttatcgaa
    10801 acaagaaatt agtgtcatgc ggtatattct tgatggcaag gataataatg acattgctga
    10861 aaaaatgttc atcagcaaca aaactgtcag cacttataaa agtcgcctga tggaaaaatt
    10921 agaatgtaaa tcactgatgg atctttacac attcgcgcaa cgtaacaaaa tcggctaatc
    10981 acatgaagtt tttaccctat ttttttcttc tctgttgtgg tctttggtcg accataagtt
    11041 tcgcagacga agattacacc gaatatcgtg gcatcagtag taacaaccgt gtcacacttg
    11101 atccagtacg tctgagcaac aaggaattac gttggttagc gagcaaaaaa aatcttgtga
    11161 ttgcagtaca caagtcccaa acggctacct tgttgcatac cgattcgcag caacaggttc
    11221 gtggtattaa tgctgattat ttaaatcttt taaaaagagc gttaaatatc aaattaacac
    11281 tccgggaata cgcagatcat caaaaagcaa tggacgcgct ggaagaaggt gaagtcgata
    11341 tagtgttatc acatttagtt acttcgccgc ctcttaataa tgacattgct gcaaccaaac
    11401 cattgataat tacctttccg gcgctggtaa ccacccttca cgactcaatg cgaccgctta
    11461 cctcaccaaa accagtaaat attgctcggg tagcaaatta ccccccagac gaggtaattc
    11521 atcaatcgtt tccaaaagca acaattatct cttttacaaa tttatatcag gcattagcat
    11581 ccgtatcagc cggacagaat gattacttta ttggtagtaa catcattacc agcagtatga
    11641 tttcccgcta tttcactcac tccttaaatg tagtgaaata ttataactcg ccgcgtcaat
    11701 ataatttttt cttgaccaga aaagaatccg tcattcttaa tgaagtactc aatagatttg
    11761 ttgatgcttt aacaaatgaa gttcgctatg aagtttcaca aaattggctt gatacaggaa
    11821 acctggcctt tctgaacaaa ccattagaac tcactgaaca tgaaaaacag tggattaagc
    11881 aacatcccga tttaaaggtg ctggaaaatc cttactctcc tccctattct atgacggatg
    11941 aaaatggctc ggttcggggc gttatggggg atattcttaa tattattacc ttgcaaacag
    12001 gtttaaattt ttctccgatc accgtttcac acaatatcca tgccggaaca cagcttaacc
    12061 ccggtggatg ggatataata cctggcgcta tttatagtga agatcgagaa aataatgttt
    12121 catttgcaga agctttcata acaacgccat acgtttttgt catgcaaaaa gcacctgaca
    12181 gtgaacaaac attaaaaaaa ggaatgaaag ttgccattcc atattattat gagctgcatt
    12241 cgcaattaaa agagatgtat ccggaggttg aatggataca ggtcgataat gccagcgctg
    12301 catttcacaa ggttaaggaa ggtgaacttg atgctctggt cgcgacacag ttaaattcgc
    12361 gttacatgat cgatcattac tatcctaatg aactttatca ttttcttatt cctggcgttc
    12421 cgaatgcatc gctttcgttc gcttttcctc gcggagaacc ggaacttaag gatattatta
    12481 ataaagcact gaatgcaatt cccccaagcg aagttctgcg cctgacggaa aaatggatta
    12541 aaatgcccaa tgtgaccatt gacacatggg acctatatag cgagcaattt tatattgtta
    12601 cgacattatc cgttttatta gttggcagta gccttttatg gggattctac ctgttacgct
    12661 cagttcgtcg tcgtaaagtc attcagggtg atttagaaaa ccaaatatca ttccgaaaag
    12721 cactctcgga ttccttaccg aatccaactt atgttgtaaa ctggcaaggt aatgtcatta
    12781 gtcataatag tgcttttgaa cattatttca ctgcggatta ctacaaaaat gcaatgttac
    12841 cattagaaaa cagtgactca ccctttaaag atgttttttc taatgcgcat gaagtcacag
    12901 cagaaacgaa agaaaatcga acaatataca cacaggtatt tgaaattgat aatggcatcg
    12961 agaaaagatg cattaatcac tggcatacat tatgcaatct tcctgcaagt gacaatgcag
    13021 tatatatttg tggttggcaa gatattactg aaacgcgtga tctaattaat gcactcgagg
    13081 tagaaaaaaa taaagcgata aaggctaccg tagcaaaaag tcagtttctg gcaacgatga
    13141 gtcacgaaat aagaacacca ataagctcta ttatgggctt cctggaactt ctgtcgggtt
    13201 ctggtcttag caaggagcaa cgggtggagg cgatttcact tgcctacgcc accggacaat
    13261 cactcctcgg cttaattggt gaaattcttg atgtcgacaa aattgaatcg ggtaactatc
    13321 aacttcaacc acaatgggtc gatatcccta ctttagtcca gaacacttgt cactctttcg
    13381 gtgcgattgc tgcaagcaaa tcgatcgcat taagttgcag cagtacattt cctgatcatt
    13441 acctggttaa gatcgaccct caggcgttta agcaggtctt atcaaattta ctgagcaatg
    13501 ctctcaaatt taccaccgag ggggcagtaa aaattacgac ctccctggtt cacattgatg
    13561 acaaccacgc tgttatcaaa atgacgatta tggattctgg aagtggatta tcgcaggaag
    13621 aacaacaaca actctttaaa cgctacagcc aaacaagtgc aggtcgccag caaacaggat
    13681 ctggcttagg cttaatgatc tgcaaagaat taattaaaaa tatgcagggc gatttgtcat
    13741 tagaaagtca tccaggcata ggaacaacat ttacgatcac aatcccggta gaaattagcc
    13801 agcaagtggc gactgtcgag gcaaaagcag aacaccccat cacactacct gaaaagttga
    13861 gcatattaat cgcggatgat catccgacca acaggctatt actcaaacgc cagctaaatc
    13921 tattaggata tgatgttgat gaagccactg atggtgtgca agcgctacac aaagtcagta
    13981 tgcaacatta tgatctgctt attactgacg ttaatatgcc gaatatggat ggttttgagt
    14041 tgactcgcaa actccgtgag caaaattctt ccttacccat ctgggggctt acagccaacg
    14101 cacaggctaa cgaacgtgaa aaagggttaa gttgcggcat gaacttatgt ttgttcaaac
    14161 cgttgaccct ggatgtactg aaaacacatt taagtcagtt acaccaggtt gcgcatattg
    14221 cacctcagta tcgccacctt gatatcgaag ccctgaaaaa taatacggcg aacgatctac
    14281 aactgatgca ggagattctc atgactttcc agcatgaaac gcataaagat ctacccgctg
    14341 cgtttcaagc actagaagct ggcgataaca gaactttcca tcagtgtatt catcgcatcc
    14401 acggtgcggc taacatcctg aatttgcaaa agttgattaa tattagccat cagttagaaa
    14461 taacacctgt ttcagatgac agtaagcctg aaattcttca gttgctgaac tctgtaaaag
    14521 aacacattgc agagctggac caggagattg ctgttttctg tcagaaaaat gactaaatag
    14581 cggctcccac aatgttcaaa tgtgggagct atttaccagc acatcttaca tttatgatga
    14641 gaactcctgg cgaatttgtt ccccatgctg gtcgagcgtt gccgctcccg gcataacatg
    14701 cgggtccgcg cagccgctga tttttatcgg attacccggc atcattattc ccccggcttc
    14761 aatcaacata tttctcgcct gagtttgtgg caatttaatg gcctcagcca cacttaataa
    14821 cggcgcgacg ggtacaccaa cttcatgtat tctggctaac caaacttcag ctgcctgcgt
    14881 ttttaacgtc cgctcaatat attgtttaag aatagcctgg ttttgtacgc gtaaaatatt
    14941 gctgctaaat cggggatcat taaccagttc cgtaagctcc agtgcctggc ataacgcaga
    15001 aaaaagcttg tcattaccac aacaaatcgt aatcggctta tcctgagtat tgaaaacatc
    15061 aaaaggtgcc atgtagggat ggcgatttcc cagacgttgt ggtgacttcc ctgtcgcgat
    15121 atatgccatc agaccatgct ccagaaaact cagcgtggca tcaaacatcg ctatatcgac
    15181 atgcgcccct ctctggctct tttcgcggcc ataaagtgca ctcactattc cgctgaataa
    15241 atagacaccg ccgcatagat ccgcaagaga tgtaccaacg cgcactggcg gagcatcagg
    15301 atatcctgtt tccatcatta tcccgctcat tgcctgaatg atggtatcgt aggcaggagc
    15361 atcttttagc ggaccggtat gtccgaaacc tgacgatgaa gcatatatga ggcgcgggtt
    15421 gatttcttga agcatttccc atgaaaaccc cagtttttcc attgtacctg ggcgaaaatt
    15481 ctcagctaat acatcagctt gtttgagcat atttataaat atacttttat cgtgatcatt
    15541 ctttaaatca agaaccacac tctctttgcc atgattaata aaactgtaat agagtgactg
    15601 tccatccaca tagggaccaa atgtgcgggt atcatcacca tgacctggtg gctcaacttt
    15661 aattaccctt gcgcccatat tacaaagaag ttgagttccg aaaggtccat taaggacatg
    15721 tgtcatatcg ataactaata agccttcaaa cggccctttg ctttcattat ttgtcatttg
    15781 cttatgcctt gaaaaaagcc gaaggtgaaa agacacttcg gcatttatgt aaaatcaata
    15841 acatgattaa ttatgaaacc agtcgactga cataaatcca caagggagcc gtgacgacaa
    15901 aacccagtac gctcacagcc aatgacgctg taccagtgcg ggtgtagaca ttaaaccggc
    15961 tggcaataat gattccggag aatgccggcg gtaatgcgcc tgccagtacc atcatctgca
    16021 aatgttcgct gttcaaatga catgccatac caacgagaag cagcgccagt ggcatcagaa
    16081 tcagcttcag gaaggtgtta taagcaattt cggcactgaa ctcgaattta tgtgcagcca
    16141 gagtcaaccc cgcagcgaat accgctaccc ctgagttagc cttcgcaatc agattaaagg
    16201 ttgggtccca tgctgccgga atttttaccc caaccaacac caggatcgtt gccagaacag
    16261 gtgcccatac aactggctcc tttgccgcag aaattaatgc gctcagatta ctattcttct
    16321 taccatccgc tcctgaggaa ggattcagca aatacagacc aataggaata gtaattgcgt
    16381 taacaataat agaaataatt gctaccacta aacctgttga tacggaatca ccataaatag
    16441 gatcgagaac tgcaaacccc aggaatccaa tggtaggtga acctgcaatt aatgcacata
    16501 cagctgcttc tgcatgggta cgtttaaaaa atttgtagca accgaaccag gagaaaaaga
    16561 aacatccgac aataaccaca agtgatacaa gggtcagacg agtgtccgca aaaatcattt
    16621 ccctgtttgc ccgagtaata gatacaaata gagccgcagg aagcgcgtag tttaatacca
    16681 gtttattaaa tgcccgagct tgatcttctg aaaaagtttc tcgtctgccg ctaaaataac
    16741 ccaacagcat gattacgata ataggcaata aatcgccaat aaaaaatgtt agcataatag
    16801 tatttccgat aagggtcccc tcaccatcgc ataatgagtt ggcaagagga gacgatgtca
    16861 gtatttaatt accagcgact tgttttgggt taagtttggt aatatggcca ctttcagttc
    16921 ctgctgcagg gtcgatgacc acattaataa tggtcggttt gcgcgactga ataccggtgg
    16981 ttaaagcatg acgaagttca tctgtcgtgg tgacgttata gccaacgcca cgaaacgcat
    17041 ccattaattt gtcatacctt gcatggtgca acagatccgt tggtgatggt gcaccagcgc
    17101 cactgagatc aacaccgtct cctctgtaga tgccgccatt attaaaaata acgatcgtca
    17161 ccggcaggtt atatcgacaa atcgtttcaa tttccatccc actgaaacca aaagcactat
    17221 caccttcaat ggcgacaacc ggagaaccag aagtcacgct agcaccgatg gcatagccca
    17281 taccgatgcc catgacaccc caggtaccac aatccagacg acgacgtggt ttatacatat
    17341 caataatatt tcgtgcatta tccagggtat ttgcaccttc attaactaaa taaatatcct
    17401 ggttctcgcg caatacgtcg cgcacagcac ttaatgcatt aaagtaattt aatggttgag
    17461 tatctgtact taatttttca tgcatttttt gtgcattttg ctgcttgtgg atatttaaaa
    17521 tatcgcgcca taccagtgga gtcgtaaatg tgttttgttt cagttctgcc agcatacctt
    17581 gcatactgga tgcaatatca ccaacgactg gcacagcaat ggggcggttg ctgtcaattt
    17641 cctgcggttc aatatccagt tgaataaact gtgtatctgc cgcccatcct tttttaccgt
    17701 gtgccaataa ccaattcagt cgtgcaccaa caagcatgac aacgtcagca tttgccaggg
    17761 caaacgaacg cgcagctgcc gcagaaagtg gatgcgtatc ttcaaggatc cctttcgcca
    17821 tagacattgg caggaatgga atctgagtac tttcaataaa ttcacgaagc tgttcatcag
    17881 cttgtgaata cgccgcgcct ttgccaagga taattaatgg ccgttcagct tttgctaaaa
    17941 gcgaaattgc gctagtgact gacttcgggc atggcaataa tgctggcgat ggattttcaa
    18001 ctttaacaat cgtggttaac gcttcgtctt tttccatcgt cgcggccagg acatttgctg
    18061 gcaaatcaag ataaactccg ccagggcgac ccgatacaga gacccggata gcgcgtgcca
    18121 atgcaatgcc aagatcctgc ggctgattaa cgcgaaatgc tgctttggca tacggttttg
    18181 ccgcattcat ttggtccagc tcttcataat caccttgctg taggtcgacg atcgcgcggt
    18241 cgctggagcc gctaatcatg atcatcggaa aaccatttac cgttgcgttg gccaatgcgg
    18301 tcaaaccatt gaggaatcct ggcgcagaaa ctgtcaggca gatccccggt ttttgggtaa
    18361 gaaaaccgct tgccgcagcg gcatagcctg ccgactgctc atgacgaaaa ccaatataac
    18421 gaatgccttc cgcctgggca tggcgtgcca tatccgtcac aggaatacct acaacaccat
    18481 aaatagtgtc aatattattc tgttttaatg cttcaacgat gatatgcata ccatctgtca
    18541 tttgaagttg atctgacatg attaataacc tcgaaatttt tataaattgg gagccagaag
    18601 cccccgttaa tatcagatgg cgtggttttg cttcattgca gcaatttcat cgtcgctata
    18661 acccagctcc tgcaatacag cagcggtatg ttcacctaat agcggcgcag ctttaatatc
    18721 cggcgtaaag gcagagaatt tcattggaca accaacggtc agatattttc cacgcaacgg
    18781 ttgttccact tcaacaacac tgccactttg gcgcaaagag ggatcaagtg aaatttcttt
    18841 catacttaaa accggtgcac aaggaatatc aaactgagtc aaataggcca ccgcttcatg
    18901 tttatcaata gtgacagtgt atttttcgat ttcagcaaaa atatcgaaaa tatgtggctg
    18961 tcgtgcatgg gctgtactgt atgccggatc ggtaatccat tctggtttgc cgatggcttt
    19021 acaggtgttt tcccagtttt gctcctgaat agtgaaataa atataggcgt taggatcggt
    19081 ttcccagcct ttacatttca ggatccagcc aggctgaccg ccaccacctg cattaccacc
    19141 gcggggaact gcatcaccaa atgtaccatt cggatactgc gggtattctt ccagataacc
    19201 caatttatcg agacgctgct ggtcacgtaa tttcacgcgg caaaggttca atacggcatc
    19261 ctgcattgac atggtgactc gttgcccacg ccccgttttt tcgcgatgca gcaaagcagc
    19321 aagtaaaccg atcagcaaat gcattccggt gttgctgtca cccaacgctg cagcgcttac
    19381 cagcggcgga ccatcccaaa aacccgtagt ggatgccgcg ccacccgctg cctgagcaac
    19441 gttttcatag gcttttacat tcacataagg cgaacactca tcaaaccctt tgatcgaacc
    19501 aaaaatcaga cgtggattga tttcttgaat atgctcccag gtgaagccca tgtgatcaat
    19561 ggcccctgga tgaaagttct cgactaagat atcagcttcg cggatcagct tttccattac
    19621 ctctttgcct tccgctgttt tggtatttaa ctcaatagaa cgtttgttac tgttaagcat
    19681 ggtgaagtaa agcgcatcga tatcaggaat atctcgcagc tggtgacgcg ttacgtcacc
    19741 aacgccggga cgttcaattt taataacgtc agcgccaaac caggccagca tttgagtaca
    19801 agatgggcca gattgcacac cggtgaaatc gagaacttta attccttgaa gtggagtt
//
LOCUS       DAPHDX010000079        19402 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPHDX010000079 DAPHDX010000000
VERSION     DAPHDX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.079"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1037
                     /gene="ompN"
                     /locus_tag="RH058_002864"
     CDS             <1..1037
                     /gene="ompN"
                     /locus_tag="RH058_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV3138428.1"
                     /translation="LYGKVDGLHYFSDNSAKDGDQSYARLGFKGETQINDQLTGYGQW
                     EYNIQANNTESSKNQSWTRLAFAGLKFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGD
                     SYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVR
                     HENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQVNHTAAGGDKADAWTAGLKYDANN
                     IYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHA
                     AGGADNPAGVDDKDLVKYADVGATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDD
                     IVALGLVYQF"
     gene            1178..1612
                     /gene="uspF"
                     /locus_tag="RH058_002865"
     CDS             1178..1612
                     /gene="uspF"
                     /locus_tag="RH058_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV3138429.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1788..2723
                     /gene="ttcA"
                     /locus_tag="RH058_002866"
     CDS             1788..2723
                     /gene="ttcA"
                     /locus_tag="RH058_002866"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV3138430.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2852..4225)
                     /gene="dbpA"
                     /locus_tag="RH058_002867"
     CDS             complement(2852..4225)
                     /gene="dbpA"
                     /locus_tag="RH058_002867"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV3138431.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4255..4428)
                     /locus_tag="RH058_002868"
     CDS             complement(4255..4428)
                     /locus_tag="RH058_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138432.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPSPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4703..5686)
                     /gene="zntB"
                     /locus_tag="RH058_002869"
     CDS             complement(4703..5686)
                     /gene="zntB"
                     /locus_tag="RH058_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV3138433.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5941..7173
                     /gene="dgcM"
                     /locus_tag="RH058_002870"
     CDS             5941..7173
                     /gene="dgcM"
                     /locus_tag="RH058_002870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV3138434.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFLHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7194..7757)
                     /gene="smrA"
                     /locus_tag="RH058_002871"
     CDS             complement(7194..7757)
                     /gene="smrA"
                     /locus_tag="RH058_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV3138435.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8087..8995)
                     /locus_tag="RH058_002872"
     CDS             complement(8087..8995)
                     /locus_tag="RH058_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3138436.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9171..10481
                     /gene="abgA"
                     /locus_tag="RH058_002873"
     CDS             9171..10481
                     /gene="abgA"
                     /locus_tag="RH058_002873"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV3138437.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10481..11926
                     /gene="abgB"
                     /locus_tag="RH058_002874"
     CDS             10481..11926
                     /gene="abgB"
                     /locus_tag="RH058_002874"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV3138438.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            11963..13489
                     /gene="abgT"
                     /locus_tag="RH058_002875"
     CDS             11963..13489
                     /gene="abgT"
                     /locus_tag="RH058_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDV3138439.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13500..14015
                     /gene="ogt"
                     /locus_tag="RH058_002876"
     CDS             13500..14015
                     /gene="ogt"
                     /locus_tag="RH058_002876"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV3138440.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14210..14962
                     /gene="fnr"
                     /locus_tag="RH058_002877"
     CDS             14210..14962
                     /gene="fnr"
                     /locus_tag="RH058_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV3138441.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15114..16064
                     /gene="uspE"
                     /locus_tag="RH058_002878"
     CDS             15114..16064
                     /gene="uspE"
                     /locus_tag="RH058_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV3138442.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16114..16371)
                     /locus_tag="RH058_002879"
     CDS             complement(16114..16371)
                     /locus_tag="RH058_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV3138443.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16615..17646
                     /gene="ynaI"
                     /locus_tag="RH058_002880"
     CDS             16615..17646
                     /gene="ynaI"
                     /locus_tag="RH058_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV3138444.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(17697..19310)
                     /gene="mppA"
                     /locus_tag="RH058_002881"
     CDS             complement(17697..19310)
                     /gene="mppA"
                     /locus_tag="RH058_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV3138445.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         4911 a         4810 c         4686 g         4995 t
ORIGIN      
        1 atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg aaagatggcg
       61 accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat caactcactg
      121 gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca aaaaaccagt
      181 catggacccg tctggcattt gccgggctga aatttgcaga ttacggttct ttcgattacg
      241 gacgtaatta tggcgtaatg tacgacatcg aaggctggac cgatatgctg cctgaatttg
      301 gcggtgactc ttataccaat gcagacaact ttatgactgg tcgagccaat ggcgtcgcga
      361 cttatcgtaa tactgatttc ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc
      421 aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac caacaacgga cgtgatgttc
      481 gccatgaaaa cggtgacggc tggggtcttt ccacaacata tgatttaggc atgggcttta
      541 gcgctggtgc ggcatacacc tcttctgacc gcaccaatga ccaggttaac catactgcgg
      601 cgggtggtga taaagcagac gcgtggactg ctgggctaaa atacgatgct aacaatattt
      661 acctggcaac catgtattca gaaacgcgta atatgacccc gtttggcgac agcgattatg
      721 ctgttgcaaa caaaacccag aactttgaag tcactgcaca gtaccagttt gattttggtc
      781 tgcgtccggc agtctctttc ctgatgtcta aaggccgtga cctgcacgct gccggtggtg
      841 cagacaaccc ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga
      901 cttactattt caataaaaac atgtccactt atgttgacta taaaatcaac ctgttggatg
      961 aagatgacag tttctacgct gccaatggca tctctaccga tgatattgtc gctttaggtc
     1021 tggtttatca gttctaaatc ctcctgcccg ctgttatggc gggctttttc tgcttattct
     1081 tcctcctttg atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg
     1141 cgttacactt tatgcagata ctaaaacagg aggttttatg aacagaacga ttcttgtccc
     1201 tatcgatatt tccgattcag aattaactca acgcgtgatt agccacgttg aggcagaggc
     1261 aaagattgat gatgcagagg ttcatttcct gacggtaata ccttcactgc cctactatgc
     1321 ctctctgggt ttagcgtatt ccgcagaatt accggcaatg gatgacctga aagcggaagc
     1381 caaatcgcaa ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt
     1441 ccatgttgag gaaggctcgc ccaaagaccg cattctggaa atggcgaaga agatccccgc
     1501 tcatatgatc atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttccaa
     1561 cgccgcagct gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct gacactaacg
     1621 cccgcacatt gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct
     1681 aatccgtacc atacacgcca ctgtttttat atcagatttc ttatgctggg cattccggca
     1741 tgacgcatac tcttctgatg ccatataacg aattgagtcg cttttaaatg tcgcaaaatc
     1801 aagaaattag taagaaagaa caatacaacc tgaacaaatt acaaaaacgt ctgcgtcgta
     1861 acgtgggcga agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt
     1921 gcctctccgg gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa
     1981 gcgcgccaat caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc
     2041 cggaacacgt tctgcccgag tatcttgaaa agctgggcgt tgagtacaag attgttgaag
     2101 agaatactta cggtatcgtg aaagagaaga ttccagaggg caaaaccact tgctcactgt
     2161 gttctcgcct tcgtcgcggt atcctttatc gtaccgcaac ggaactgggg gcgacgaaga
     2221 tcgcgttggg tcaccatcgt gacgatatcc tgcaaacgtt gttcttaaat atgttctacg
     2281 gcggcaagat gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtta
     2341 ttcgtccgct ggcctactgc cgcgagaaag atattcagcg atttgccgat gcaaaagcgt
     2401 tcccgattat tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg
     2461 ctgacatgtt gcgtgactgg gataaacgtt atccagggcg tatcgagacg atgttcagcg
     2521 cgatgcagaa tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat tttaaaggca
     2581 ttacccacgg ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc
     2641 cactacaacc ggcgggctgg cagccagaag aagatgaaaa tcagttggat gagttacgac
     2701 tgaatgtggt tgaagtgaaa taaccaggat agcgcccgat gcgcaagcgt atcgggctac
     2761 tcttatggag gccggataag acgcggccag cgtcgcatcc ggcaatcccg aataagatgt
     2821 ttactcttgc acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt
     2881 ttcctttaat cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga
     2941 ccgcgacata gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc
     3001 cgatatctcc tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc
     3061 catcgataca caacgttgcc atttctgctt ccagcggcac aatggaacta ttagctggcg
     3121 gcgtttgcca gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt
     3181 cttccggagc acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga
     3241 tgcgatgtac atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg
     3301 atttaatatc cagaccacgc gcagcaacat cagtagcaac cagtacacgg gcgctaccgt
     3361 tagcaaaacg taccagagtc tgatcgcgat cgcgttgctc cagatcgccg tgtaacgaca
     3421 atgcactttg ccctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat
     3481 tgcaaaacac cacgcaagag gatggctgat gcaagcttaa taaccgttgc aacagaggaa
     3541 ttttgccttt gctggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg
     3601 agtcaatttc aatcgccaaa ggatcgcgtt gtactcgtcc gctgattgcg gcgatggctt
     3661 ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat
     3721 catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag
     3781 tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa cgacccggcg
     3841 ttgccacgat aatatgcggc gcatgttgca acgaatcacg ctgcataccg aacggttgac
     3901 caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac
     3961 ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata
     4021 gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccgg
     4081 ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca
     4141 ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa
     4201 cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagttagca
     4261 ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt cttcatcggg
     4321 tgatgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa caggatcgcc
     4381 aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat ttccctccgg
     4441 tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa aaagccgact
     4501 catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa aaggaagtaa
     4561 gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag ggagatattg
     4621 ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt ttgtatacag
     4681 acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat aaagcaacac
     4741 caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc cacccgccgc
     4801 cagggatccc accaaggttg acgccaaata accctgtcag aaaggtactg ggtaaaaaga
     4861 ccattgccat caatgacatt gtataggtac gacgagctaa attttcctgc atcacctgag
     4921 cgatttcatc cgccatcaca ccagtccgtg ctatacaggc gtcgatttcg tcaaggccgc
     4981 gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc atccacggca
     5041 aacgttcact ggcaagacga gcatagacat cacgttgcgg tgccatatag cgacgcatca
     5101 cgattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc tgatcaagga
     5161 gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattcg ctggaatgat
     5221 cggtcaacgc atcgcacaca tccaccagcc accctccgca atcggtcgga cccgtgccct
     5281 cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa
     5341 ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt tcatcggtgc
     5401 tgccgtttat acagcgcaat gtaataagcg tgccttcacc gagacggctg actcggggcc
     5461 gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt gttgtcgcca
     5521 gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga tgcgcttcat
     5581 caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc atccaggcaa
     5641 atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg cctccatcat
     5701 caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc atcaccccat
     5761 taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa aatgcttttc
     5821 cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt atctgatcat
     5881 atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa tgggatctca
     5941 atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg gatcgtttcg
     6001 ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat gcaagacctc
     6061 acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga actgccgaaa
     6121 tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa
     6181 gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga aacagaaccg
     6241 gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg
     6301 gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac taactctgca
     6361 ccaatgttgt tgattgaccc ctcacgagat ggccaaatcg tcgatgctaa cctcgccgcg
     6421 ctcaatttct atggttataa ccatgaaacg atgtgccaga aacatacctg ggaaattaat
     6481 atgctcgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc cggcggtcat
     6541 aaacctttga attttcttca taaactggcg gatggatcta ctcgtcatgt gcagacctat
     6601 gccgggccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca tgatattact
     6661 gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc gatgaccgga
     6721 ttactgaatc ggcgacagtt ttatcacatt accgagccag gccaaatgca gcacctcgcc
     6781 atcgcccagg attacagctt gttgctcatc gacaccgatc gttttaaaca cattaacgat
     6841 ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgcac cctcgaaagt
     6901 tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt cttattgcta
     6961 ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgtgt aagcgtggca
     7021 aaagtgagta tttcgggctt accacgtttt accgtcagca ttggtgtggc gcatcacgaa
     7081 ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta tcgggcgaaa
     7141 aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg agatcaacgg
     7201 ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt acgtagtgcg
     7261 acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc ctgaacatca
     7321 tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg cgatttatca
     7381 tcccgtcctt tgccatgaat aatcagcacg ttacgcaaac catcagccat ggcttgttga
     7441 atgaaactga acatcatttt gcggcactct tccaccggct ggcgcaaaag attcaggctc
     7501 gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgttg caacccttcc
     7561 cgccgaaact ccagcggctg acttagtggg atgatgtcga gaaatccggt ggtgaggaaa
     7621 ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt tggatgccag
     7681 tggacatcgg tagcacgttt cagcggctgg acatcttcca tggcgtcaag aaacagcgat
     7741 ttgtcgtcaa ggttcatgtt acatcctccc gcaattaaga gcgcgatatg ataaccagac
     7801 cgggtcggtc caacaacgta ttacccaaaa ttccagtaat aagttccaaa tattgccgat
     7861 attttaagca aaatgcttat gcatgattat tcattcacga tattaataat gtaacttata
     7921 ttttcgtgaa atctgtcact gaagaaaatt ggcaactaaa ggttaaaacc gttataacac
     7981 agtcaccggc gcagaggaga caatgccgga cttaagacgc ggatgcactg ctgtgtgtac
     8041 tgtagagtct ggcggatgtc gacagactct atttttttat gcagttttaa ctttgcagat
     8101 agccgcattc tcgccggaat tgcgtgatta atgacgcggt cagtggtgtc tggcgactat
     8161 cacgccgctg aatcaaataa taggccgctt tcggtaaaat ttcgctaacc ggcaacatca
     8221 ccagcccctg tccgtgcaag ggatcgcagc ccatttcttc aggcagtatg ctgagaaaat
     8281 cgctttttgc caccagactg atacaggctg agaacgtctc gcagactaca ccgacctgtg
     8341 gcgtttgcgc ctgatcgtca agcaattcac tcaactgttt gtagtaactg ccgtgtggcg
     8401 tcggcattgt ccagctgtaa tccagtaact gtttgatcga acgggcacca atggcggggt
     8461 gtcccgggcg gcaaaagatc gcgaattgct tttccagtaa tttctcaaaa gtaaattcgt
     8521 ggtcgtacgg tccctgataa taggtattga tggtgaaatc caattctccc tgacgcaatt
     8581 cattaatcat cgacaccagt tgcccttcca taatgcgtac ttttacctgc ggatgctgct
     8641 gatgaaaacg agatatgaca gctggcatca gactgcggga aatactggcc cccatgccga
     8701 tattaatctg ccctgccagt tgcccttgtc gttggcgaat ttcctcttgc gctgcgcgca
     8761 gctcttcaag aataagactg gcgtgctgat aaaaactttc accggcatca gttaacgtca
     8821 cgcctttact acggcgaaaa aagagttgcg ccgctaatcc ttcttccagc tcctgaatag
     8881 atttactcag tgccggttgc gacatattca acattcggct cgctccgcga atgctgccct
     8941 gacgagccac ttcaacaaaa gcacgaattt gatgaatttt tacctgaaaa gccatgacgc
     9001 caccgataac cgttatttat cagaccaaag aaactggcat ctactttaat gcagatgatt
     9061 gtgtcagggt aatttatgaa cggttaaaac tgtgaaaaat cagttagtga taagtaaaaa
     9121 ctatcgctac gtgaaccggg tcacactttt tactgatgac gggaaaggtt atggagtctt
     9181 tgaatcaatt tgttaattcg cttgccccaa aattatcgca ctggcgacgt gattttcatc
     9241 actatgcaga gtctggctgg gtggaattcc gcactgccac ccttgttgcg gaagaattgc
     9301 agcagctcgg ctattcactg gcgctgggcc gcgaagtagt taatgaaagt agccggatgg
     9361 gactacctga tgaactcact ctacaacgcg aattcgagcg cgctcgtcaa cagggtgcgc
     9421 tagcacaatg gattgcggtt tttgaaggtg gtttcactgg tatcgtcgcc accctggata
     9481 ccggtcgccc cggtccggtg atggctttcc gtgtcgatat ggacgcgctg gatctcagtg
     9541 aagagcagga tgtcagccat cgcccctacc gcgacggttt tgcgtcatgt aacgccggaa
     9601 tgatgcatgc ctgtggtcat gatggacata ccgccattgg gcttgggctg gcgcataccc
     9661 ttaaacaatt cgagtccgga ctacatggcg tcatcaaact gatttttcag cctgcagagg
     9721 aaggtacgcg tggcgcgcgg gcgatggtcg atgcaggtgt cgtagatgat gttgattatt
     9781 ttactgccgt gcacattggc actggcgtac ctgcgggcac cgtggtgtgc ggcagtgata
     9841 attttatggc aaccaccaaa tttgacgcgc acttcaccgg gaccgccgct cacgcaggcg
     9901 caaaaccaga agacggtcac aatgccttgt tggcggcagc acaagccact cttgcactgc
     9961 atgcaatcgc cccgcacagc gaaggagctt ccagagtaaa cgtgggcgtt atgcaggcag
    10021 gaagcggtcg taacgttgtt cctgcctcgg cgttgctgaa agtggaaaca cgcggggcca
    10081 gcgacgtcat taatcaatat gtttttgacc gtgcacaaca agcgattcag ggcgcagcaa
    10141 ccatgtatgg tgtcggcgtt gaaactcgtc tgatgggtgc agctaccgcc agttctcctt
    10201 cgccgcaatg ggtcgcatgg ttgcaaagtc aggcggctca ggtcgcgggg gtcaatcagg
    10261 ccattgaacg tgttgaagcg cctgcgggtt ccgaagatgc cacattaatg atggcccgcg
    10321 tgcagcaaca tcaagggcaa gcctcctacg tggtgtttgg cacacagctg gcggcaggtc
    10381 atcacaacga aaaattcgat tttgacgagc aggttctcgc tattgccgtc gaaacgctgg
    10441 cgcgcaccgc gctcaatttt ccctggacgc gaggtatctg atgcaggaaa tctatcgttt
    10501 tatcgacgat gcgattgaag ccgatcgcca acgttatacc gatattgccg atcaaatctg
    10561 ggatcatcca gaaacacgtt ttgaagagtt ctggtcagcg gagcatctgg cttcggcgct
    10621 ggaatctgca ggcttcaccg ttacccgcaa cgtaggcaat atcccaaatg cctttattgc
    10681 ttcgtttggt caaggcaaac cggttatcgc cctgctggga gaatatgacg ccctggcagg
    10741 tttaagtcag caagcaggtt gcgcgcaacc tacatccgtg acgcccggtg aaaatggtca
    10801 cggttgcgga cacaatttgc tgggaaccgc cgcctttgcc gctgcaatag ccgtcaagaa
    10861 atggctggaa caatatgggc aaggcggcac agtgcgcttt tatggttgtc ctggcgaaga
    10921 aggcggctcg ggtaaaacgt tcatggtccg cgagggggta tttgatgatg tggatgcggc
    10981 actcacctgg cacccggaag cctttgccgg tatgttcaat acccgcacgc tggcaaacat
    11041 tcaggcatca tggcgcttta aagggatcgc agcacatgcc gcgaattccc ctcatttggg
    11101 acgaagcgcc cttgatgccg taacgttgat gaccactggc accaacttcc tcaacgaaca
    11161 tattattgaa aaagcgcgcg tacactatgc catcacagat agcggcggga tctcgcccaa
    11221 cgtggtccag gcgcaggcag aagtgcttta tcttatccgt gcccccgaaa tgaccgacgt
    11281 gcagcatatt tatgatcggg tcgccaaaat cgccgaaggt gcggcattga tgaccgaaac
    11341 cacggttgaa tgccgcttcg acaaagcctg ttccagttat ctcccgaatc gcaccttaga
    11401 aaatgccatg taccatgccc tatcccattt tggtaccccg gaatggaact ccgaagaact
    11461 ggcttttgcg aaacaaattc aggctacgct cacccccaac gatcggcaaa acagtctgaa
    11521 taatatcgcc gcaaccggtg gcgaaaacgg caaggttttt gcactacgtc atcgtgaaac
    11581 ggtactggcg aatgaggtcg ctccatatgc cgccaccgat aacgtgcttg cggcatcgac
    11641 tgatgtcggc gacgtcagtt ggaaactgcc tgttgcccag tgtttcagcc cctgttttgc
    11701 cgtcggtaca ccgctacata cgtggcaact ggttagccag gggcgaacat ctattgctca
    11761 taaaggaatg ctgctggcgg cgaaaactat ggcagcaacc acagtcaatc tcttcattga
    11821 ttcagggcta ttgcaagaat gccaacaaga gcatcagcaa gttacggaca cgcaaccgta
    11881 tcactgccct atcccgaaaa acgtgacacc gtcaccttta aaataacaac aacaacgcaa
    11941 acacaacaac cgaggaatgc ccatgagtat gtcatccata ccgtcgtcct cccaatccgg
    12001 gaagctctat ggctgggtcg aaagaattgg taacaaggtt ccccatcctt ttttgctctt
    12061 tatctatttg ataatcgtac tcatggtgac gacggcaatt ttgtcagcct ttggcgtcag
    12121 tgcgaaaaac ccgaccgatg gtacgccggt ggtggtgaaa aacctgctca gtgtggaagg
    12181 attacactgg tttttaccca atgtgattaa aaactttagc ggttttgctc cacttggagc
    12241 gatcctggcg ctggttttag gtgccggcct agcggagcgc gtcggcttac tgccagcgct
    12301 aatggttaaa atggcatcgc atgttaatgc ccgctacgcc agttatatgg tgctgtttat
    12361 tgcttttttc agccacattt cttccgatgc ggcgttagtg atcatgccac cgatgggtgc
    12421 gctgattttt ctggcggtgg gcaggcatcc agttgcaggt ttactggctg ccattgcagg
    12481 cgtaggttgc ggctttacgg ctaatttact gattgtcaca accgacgtgt tgctgtcggg
    12541 gatcagcacg gaagcggcgg ctgcgttcaa tccgcaaatg cacgtcagtg taattgataa
    12601 ctggtatttt atggccagct ccgtagtcgt actgacgatt gttggcggcc tgataaccga
    12661 caaaatcatc gagccacgat taggtcaatg gcagggaaac agcgatgaga aactgcagac
    12721 attgaccgaa agtcagcgtt ttggtttacg catagcaggt gtcgtatcgc tactttttat
    12781 tgctgcgatc gcgctgatgg tgatcccgga aaacgggata ttgcgcgatc cgattaatca
    12841 caccgtgatg ccatcaccct ttattaaagg tatcgtgcca ctgatcattc tttttttctt
    12901 tgttgtctcg ctggcttatg gcatcgctac ccgcacaatt cgacgtcagg cggatttacc
    12961 gcatttaatg attgaaccga tgaaagagat ggcgggattt atcgtgatgg tttttcccct
    13021 cgcccaattt gtcgccatgt ttaactggag caacatgggg aaattcatcg ccgtggggct
    13081 gaccgatata ctggaaagtt cagggcttag cggcataccg gcgtttgtcg gtctggcgtt
    13141 gctttcctct ttcttatgca tgtttattgc cagcggttcc gcaatctggt cgattctggc
    13201 ccccattttc gtaccaatgt ttatgctact tggctttcac ccggcatttg cgcaaatcct
    13261 ctttcgtatt gccgactcat ccgtattgcc tttagcgccg gtatctcctt ttgtcccact
    13321 gtttcttgga ttcctgcaac gctacaaacc agacgcgaaa ctgggtactt actattcgtt
    13381 agtcttgccc tatccgctta tctttttggt ggtatggctg ctgatgttgc tggcgtggta
    13441 tcttgttggt ctgccgatag gtccggggat ttacccacgt ttgtcttaag agagaacgga
    13501 tgctgagatt acttgaagaa aaaattgcca cgccactggg tccactgtgg gtgatttgcg
    13561 atgagcaatt tcgcctgcgg gcggttgaat gggaagagta cagcgaacgc atggtgcagc
    13621 tgctggacat ccattatcgc aaagaaggct atgagcgcat ttctgccacc aacccaggcg
    13681 gtttaagcga caagcttagt gattattttg ccggtaatct tagcattatt gatacgcttc
    13741 ccactgccac aggggggacg ccatttcagc gcgaagtctg gaaaactcta cgcactatcc
    13801 cctgcgggca ggtaatgcat tacggccaac tggctgaaca attgggccgc cctggcgcgg
    13861 cgcgtgccgt tggtgcggca aacggatcga atcccatcag catcgtcgta ccttgccatc
    13921 gggttattgg ccgaaacggc accatgaccg gatatgcagg cggagttcag cgaaaagagt
    13981 ggttattgcg ccacgaaggt tatcttttgc tgtaaacatt aaacaatttg tgccagcttg
    14041 ttcacacttt tatgtaaagt tacccttaac aacttaaggg ttttcaaata gatagacata
    14101 tatttacatc tgatatcgga attctctgct gttaaggttt gcttagactt acttgctccc
    14161 taaaaagatg ttaaaattga caaatatcaa ttacggcttg agcagaccta tgatcccgga
    14221 aaagcgaatt atacggcgca ttcagtctgg cggttgtgct atccattgcc aggattgcag
    14281 catcagccag ctttgcatcc cgttcacact caacgaacat gagcttgatc agcttgataa
    14341 tatcattgag cggaagaagc ctattcagaa aggccagacg ctgtttaagg ctggtgatga
    14401 acttaaatcg ctttatgcca tccgctccgg tacgattaaa agttatacca tcactgagca
    14461 aggcgacgag caaatcactg gtttccattt agctggcgac ctggtgggat ttgacgccat
    14521 cggcagcggc catcacccga gcttcgctca ggcgctggaa acctcgatgg tatgtgaaat
    14581 cccgttcgaa acgctggacg atttgtccgg taaaatgccg aatctgcgtc agcagatgat
    14641 gcgtctgatg agcggtgaaa tcaaaggcga tcaggacatg atcctgctgt tgtcgaagaa
    14701 aaatgccgag gaacgtctgg ctgcattcat ctacaacctg tcccgtcgtt ttgcccaacg
    14761 cggcttctcc cctcgtgaat tccgcctgac gatgactcgt ggcgatatcg gtaactatct
    14821 gggcctgacg gtagaaacca tcagccgtct gctgggtcgc ttccagaaaa gcggtatgct
    14881 ggcagtcaaa ggtaaataca tcaccatcga aaataacgat gcgctggccc agcttgctgg
    14941 tcatacgcgt aatgttgcct gatttttccg cataactcac tatccttctg tcatatcatt
    15001 aaatttttct gatttattga tctggcagaa ggttcatcac tgtttcattc accagatatg
    15061 ggttaatctt ttaattacaa actgcgttga cagttgttgt aaggagaccc tgtatggcta
    15121 tgtatcagaa catgctcgtt gttatcgatc ctaaccagga cgaccaacca gcattgcggc
    15181 gagctgttta tttacatcaa cggattggtg gcaaaattaa agcctttttg ccgatctatg
    15241 acttctcata cgaaatgacc accctgctct ccccggacga acgtacggct atgcgtcagg
    15301 gcgtcatcag ccagcgtaca gcctggatcc acgagcaggc aaaatattat ctcaatgctg
    15361 gcgttcccat tgaaattaaa gtggtctggc ataaccgtcc tttcgaagcc atcattcagg
    15421 aagtgatcag cggcgggcac gatttggtgc taaaaatggc gcaccaacat gaccgtctgg
    15481 aagcggtgat ttttacgcca acggactggc atctgttacg caaatgccca agcccggtgt
    15541 ggatggtgaa agaccagccg tggccggaag gaggcaaggc gctggtggcg gtgaatctcg
    15601 ccagtgaaga gccgtatcat aatgcgctca atgaaaaact ggtcaaagag acgatcgaac
    15661 tggcagaaca agtcaaccat accgaagttc atctggttgg cgcttatccg gtaacgccaa
    15721 ttaatatcgc gattgaactg ccggaatttg atccgagcgt ttataacgat gccattcgtg
    15781 ggcaacattt gctggcaatg aaagctctgc ggcagaaatt cggcattaat gaaaacatga
    15841 cgcacgtaga aaaaggtctg ccagaagagg tgattcctga tttggcggaa cacttacagg
    15901 cggggattgt ggttctgggc acggtaggac gcaccggtat ttcagcagca ttcctcggca
    15961 acacggcgga acaggtgatt gatcatcttc gctgcgatct gttggtcatt aaacctgacc
    16021 agtatcagac acccgttgaa ctggatgacg aagaagacga ttaacgactc ttcaataact
    16081 gacaaggccc gagttatcgg gcctattttt tacttactgc tcttcgccgc caaggaaata
    16141 aattcccaac gggattgcca gcaacacagt aaagaccagg ctataaacaa aaatcatcga
    16201 tgactgaatc acatacattg atgtcgtcca ctcagacagc ggaatattgt actgttctat
    16261 aacgccgccg atagtcgcgc gagtcacaac cgacgccgca ataatgaaaa ccgccaacaa
    16321 accaaagaga aatttcttcc ctttcgctga cttcagtttc gccataatca tgatgtaatc
    16381 cttgtgcaga caaatttctt gttacctaaa cgtaacaata ccatgacatt tcaccgttgt
    16441 ctgctctgaa aacctcacct cttagctgga tttacaatca acgcattgat tagttagcta
    16501 atgttgctac ttccttccct gactattgat gatctgaatg aattgtcgcg gtaaaatagc
    16561 cccgccaggg gtttccggca tgacgacaat tatctggagg agcctgataa aacaatgatc
    16621 gctgaactgt ttacaaataa tgcgcttaat ctggtcatta ttttcggtag ctgcgcagca
    16681 ttgattctga tgagcttttg gtttcgccgc ggaaatcgta aaagaaaagg ttttttattc
    16741 catgcggtgc aatttttaat ctacaccata attatcagtg ctgttggtag cattattaat
    16801 tatgttatag aaaactataa actcaaattt atcaccccag gcgttatcga ttttatctgt
    16861 acgtccctga ttgcggttat tctgacgatt aagctatttt tgctgattaa tcagtttgaa
    16921 aaacagcaga ttaaaaaagg tcgcgatatc accagtgcgc ggattatgtc gcgcattatc
    16981 aaaatcacca ttattgtggt gcttgttctg ctttatggcg aacatttcgg catgagcctt
    17041 tctggcttgc tgacctttgg tggtattggt ggtctggctg tcggtatggc cggtaaagat
    17101 attctgagta atttcttttc cgggatcatg ctctatttcg accgtccttt cagtattggc
    17161 gactggatcc gttcaccgga cagaaatatc gaaggtacag tagcggaaat tggctggcga
    17221 attaccaaaa ttacgacctt tgataatcgt ccattgtacg taccgaactc gctgttttca
    17281 tcgatcagcg tagaaaaccc aggacgaatg accaaccgcc gcattaccac gaccattggt
    17341 ttacgttatg aggatgcggc aaaagtgggc gttattgtcg aagccgtacg tgagatgctg
    17401 aaaaatcacc cggccatcga ccagcgacaa accttactgg tttatttcaa ccagtttgcc
    17461 gactcgtcat tgaatattat ggtttattgc tttaccaaaa ccacggtatg ggctgagtgg
    17521 cttgctgcac agcaagacgt ttatttgaag attatcgata ttgtacagtc acacggcgcg
    17581 gactttgcct tccccagcca gacgctgtat atggataaca ttacaccgcc ggaacagggt
    17641 cgctaaatca gacatcactg gcgttatcca caagggtaac gccagttccc atcacatcaa
    17701 tgcttcacaa tatacatagt ccggctgtac gccacatctt ccgggttatt aatgggataa
    17761 cctttcagcc acggcttgat taatcgtcca ttggtatatt gataaattgg tgcaatcggt
    17821 gcttgctcca tgaggatttt ttctgccgcg ttgtaatcgg cattacgcgc tttaacggta
    17881 ttttccgtcg atgcctgggc cagaactttg tcgtatgccg gattgttaaa gcgtgaaata
    17941 tttcctgaat gcgttgacgt taataatgtc aggaaagtgg agggttcatt ataatccccc
    18001 acccatgagg cacggataac atcaaaattg ccggtgttac ggctatcgat ataggttttc
    18061 cattcctgat tttgcaattt aacatctacg ccaaggttct ttttccacat cgatgctaca
    18121 gcaatcgcaa ttttttgatg gttttctgaa gtgttataca aaagcgtcag cttcagcggt
    18181 ttttgcggac cataaccagc tgcgctcaac aaagtttttg cctgtgcatt cagttcttcc
    18241 tgactcattt gttcaaacgg cgaaggttcc ggcgtaaatc cggcggtaac atctggtgta
    18301 aaatgccacg ctggcttttc gcccgttccc aacacttttt cggtcatcag gcggcgatct
    18361 atcgtcatac ttaatgccag gcgaacgcgc tgatctgccg tcggcccttt ttgcgtgtta
    18421 aacgcataat aataggtccc gagctgtggc ggcgtataaa cctgccccgg aatatccttc
    18481 aacagcttct gatacatatt tttcgggaag gattcggtga tatcaatatc ccccgcgagg
    18541 taacgcttag tggctgcgga ttcctgatta attggcagga aggtcacttt ttgcagtacc
    18601 gttttggcgt tatcccaata atgggtattc ggtaccacga ccagtttttc attgactacg
    18661 cgatctttaa gaacataagc gccattgccg atcagatttc cgggtttcgt ccactcttta
    18721 ccgctttcta cgttggcttt ttgcaccggg aagaaggcaa agttagcggt taaattcaca
    18781 aaccacggca acggtttatc aagctgaatt ttcaaggtat gggcatcaac tgcggtgacg
    18841 ccaagctggt caggcgtagc tttaccatca ataatcgcct gtgcgttgtt gattcccgcc
    18901 agcgcggcaa accatgcaaa tggcgatagc gttttcgggt ccaccagacg ctgccagctg
    18961 tagacaaaat cttgcgccgt taccggtgtg ccatccgccc attttgcgtt atcgcgcagg
    19021 gtaaaagtcc agatacggtt gtcattactt ttccactgag tcgcaacgcc ggggacaatc
    19081 tcccctttct cgttctgatt caccagtcct tcaaacagat cgcgaatgac ctgaatctct
    19141 ggcaggccca cggctttagc gggatccagc gacgcaggct catctttaat gtggcgcacc
    19201 agctcctgct tctctgccag tactgtgccg ctcggaactt ctgcagcata cgaaagagaa
    19261 atgctgctga ccaacagcgc acaacacgtg actgaaacag agtgcttcat aagtttccct
    19321 ctaaccgaca ttaaaattga tgcgtaatta tttgtttcgc cttaaagaaa tgcaaacaac
    19381 ttccatcaaa aaagtgacac ag
//
LOCUS       DAPHDX010000080        19311 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPHDX010000080 DAPHDX010000000
VERSION     DAPHDX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.080"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2922
                     /gene="ehaG"
                     /locus_tag="RH058_002882"
     CDS             <1..2922
                     /gene="ehaG"
                     /locus_tag="RH058_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HDV3138446.1"
                     /translation="FTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATN
                     TTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDGTVTAT
                     SSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSS
                     FSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSY
                     VVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGA
                     FSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTI
                     TAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGS
                     ISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLA
                     FNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRD
                     TSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGA
                     VATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAI
                     GSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVA
                     AGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGST
                     KYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSSTNQRR
                     ITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVS
                     AGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQ
                     AYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQ
                     W"
     gene            3291..4946
                     /gene="lldP"
                     /locus_tag="RH058_002883"
     CDS             3291..4946
                     /gene="lldP"
                     /locus_tag="RH058_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV3138447.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4946..5722
                     /gene="lldR"
                     /locus_tag="RH058_002884"
     CDS             4946..5722
                     /gene="lldR"
                     /locus_tag="RH058_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV3138448.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            5719..6909
                     /gene="lldD"
                     /locus_tag="RH058_002885"
     CDS             5719..6909
                     /gene="lldD"
                     /locus_tag="RH058_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV3138449.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            7095..7568
                     /gene="trmL"
                     /locus_tag="RH058_002886"
     CDS             7095..7568
                     /gene="trmL"
                     /locus_tag="RH058_002886"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV3138450.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7621..8442)
                     /gene="cysE"
                     /locus_tag="RH058_002887"
     CDS             complement(7621..8442)
                     /gene="cysE"
                     /locus_tag="RH058_002887"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV3138451.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8522..9541)
                     /gene="gpsA"
                     /locus_tag="RH058_002888"
     CDS             complement(8522..9541)
                     /gene="gpsA"
                     /locus_tag="RH058_002888"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV3138452.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9541..10008)
                     /gene="secB"
                     /locus_tag="RH058_002889"
     CDS             complement(9541..10008)
                     /gene="secB"
                     /locus_tag="RH058_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV3138453.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10071..10322)
                     /gene="grxC"
                     /locus_tag="RH058_002890"
     CDS             complement(10071..10322)
                     /gene="grxC"
                     /locus_tag="RH058_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV3138454.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10463..10894)
                     /locus_tag="RH058_002891"
     CDS             complement(10463..10894)
                     /locus_tag="RH058_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV3138455.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGLQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11139..12683
                     /gene="gpmM"
                     /locus_tag="RH058_002892"
                     /gene_synonym="pgmI"
     CDS             11139..12683
                     /gene="gpmM"
                     /locus_tag="RH058_002892"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV3138456.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12693..13976
                     /gene="envC"
                     /locus_tag="RH058_002893"
     CDS             12693..13976
                     /gene="envC"
                     /locus_tag="RH058_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV3138457.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13980..14939
                     /locus_tag="RH058_002894"
     CDS             13980..14939
                     /locus_tag="RH058_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV3138458.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(14926..15960)
                     /gene="waaH"
                     /locus_tag="RH058_002895"
     CDS             complement(14926..15960)
                     /gene="waaH"
                     /locus_tag="RH058_002895"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV3138459.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16199..17224)
                     /gene="tdh"
                     /locus_tag="RH058_002896"
     CDS             complement(16199..17224)
                     /gene="tdh"
                     /locus_tag="RH058_002896"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV3138460.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17234..18430)
                     /gene="kbl"
                     /locus_tag="RH058_002897"
     CDS             complement(17234..18430)
                     /gene="kbl"
                     /locus_tag="RH058_002897"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV3138461.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGMIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(18705..>19311)
                     /gene="yibB"
                     /locus_tag="RH058_002898"
     CDS             complement(18705..>19311)
                     /gene="yibB"
                     /locus_tag="RH058_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HDV3138462.1"
                     /translation="IRSRIEKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFV
                     NKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTSQQ
                     QVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYK
                     RPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         4751 a         5106 c         4954 g         4500 t
ORIGIN      
        1 ttcacggcag ctcatggcac cgagaccacc agcaaaatca ccaacgttaa agatggcgac
       61 ctgacgactg gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc
      121 gtggcgacga ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag
      181 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact
      241 gcagctcatg gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact
      301 gcaaccagtt ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc
      361 acctacttcg gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac
      421 aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca
      481 tttagcacgt ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc
      541 aaacacggta ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca
      601 gactccagtt ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt
      661 gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac
      721 accattgcta atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact
      781 cttgacgacg ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct
      841 cacggaaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct
      901 gccagcagcg acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat
      961 gcgctgggtg gtgacgcaga agtcaacgct gacggcacca tcaccgcacc gacttacacc
     1021 attgcgaacg ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc
     1081 ttgctgtggg atgagactgc caatggcggt gctggagcct acaatgccag ccatgacggt
     1141 aaagccagca tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca
     1201 gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc
     1261 aatcagctcg ctggtaacac cgacgcaacc tatatccaag aaaacggtgc gggtattaac
     1321 tatgtgcgta ctaacgacga cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc
     1381 gctacagcta taggttataa ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag
     1441 ggcagctaca gcgacgttga tacgggtatc gccctgggta gcagctctgt ttccagccga
     1501 gtgattgcca aaggctcccg tgacaccagc ataacggaaa atggcgttgt tattggttac
     1561 gacaccacgg atggcgaact gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt
     1621 caaatcatca acgtagccga tggttccgaa gcccatgacg ccgttacggt tcgtcaattg
     1681 cagaatgcga ttggtgcggt cgcaaccacg ccgactaaat acttccacgc taattcaacg
     1741 gaagaagatt cactggcagt gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg
     1801 aatggcgata aaggtattgg tatcggttat ggtgcctacg tggacgcgaa tgcacttaac
     1861 ggcattgcca ttggtagcaa tgcgcaagtc attcatgtca acagtattgc gataggtaat
     1921 ggttctacga ccactcgtgg cgctcaaacc aattataccg cctacaacat ggacgcaccg
     1981 cagaactctg tcggtgaatt ctcagtcggt agtgcggatg gtcaacgtca gatcactaac
     2041 gtcgcagcag gttcggctga taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat
     2101 gcgcaggttt cccagaatac ccagagcatt actaacctgg ataatcgggt aacgaatctt
     2161 gattcacgcg tcaccaatat cgaaaacggt attggcgata tcgtcaccac cggtagcacc
     2221 aagtacttca agaccaatac cgatggtgta gatgccagcg cgcagggtaa agatagcgtc
     2281 gcgattggtt ctggctccat tgctgccgcg gataacagcg tcgcactggg tacagggtct
     2341 gtggcaaccg aagaaaatac gatctctgta ggctcatcta ctaatcaacg tcgtatcacc
     2401 aacgtagccg caggtaaaaa cgacaccgat gctgttaacg tggcacagtt gaagtcttcc
     2461 gaagctggcg gtgtgcgtta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc
     2521 accctcggtg gcggcaacgg cggtacgact cgtatcagca acgtctccgc tggcgtcaac
     2581 aacaacgacg cggtgaatta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac
     2641 accgatcagc gaatggttga aatggataac aaactgtcta aaactgaaag caagttgagc
     2701 ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttatac accgggtgcc
     2761 agcatggctt ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta
     2821 tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag
     2881 ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg
     2941 tgactaaggg caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc
     3001 tccgggtcaa caatctaagg gcaattctct gatgaggatt gccctttttc tttaccagac
     3061 atctcccccc acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa
     3121 ttccccaatt aaaactcaca tcaatattgc caatacataa catttagtta accattcatt
     3181 gtcattatcc ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag
     3241 atgagcaaca gactcattac acgatgtgcg tggactccag gagacctgca atgaatctct
     3301 ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc
     3361 ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct
     3421 cgtggacggt ggcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta
     3481 acgcgctggc ctcggtggtt tatggtttct tctacgggtt gtggcccatc gcgtggatca
     3541 ttattgcagc ggtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc
     3601 gctcgtctat tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct
     3661 gtttcggcgc gttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg
     3721 cattgctggt cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta
     3781 acaccgcgcc agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa
     3841 caggcatcga cagctttgag attggccaga tggtggggcg gcagctaccg tttatgacca
     3901 ttatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtatc aaggagacgt
     3961 ggcctgcggt cgtggttgcg ggcggctcgt ttgccatcgc tcagtacctt agctctaact
     4021 tcattgggcc ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc
     4081 tgttcctcaa acgctggcag ccagtgcgcg tattccgttt tggtgatttg ggggcgtcac
     4141 aggttgatat gacgctggcc cacaccggtt acactgcggg tcaggtgtta cgtgcctgga
     4201 caccgttcct gttcctgaca gctaccgtaa cactgtggag tatcccgccg tttaaagccc
     4261 tgttcgcatc gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg
     4321 ataaactggt tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt
     4381 ttaagtttga ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga
     4441 ttgtctggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag
     4501 aactggctct acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact
     4561 attccggact gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct
     4621 tcttctcgcc gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta
     4681 acgccctgtt cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt
     4741 tgctggttgc cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta
     4801 tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta
     4861 ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt
     4921 atgtcttaac gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt
     4981 tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc
     5041 cgctgagcgc caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct
     5101 ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg
     5161 ctggcgtcat gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc
     5221 cgatgatccg gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac
     5281 cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt
     5341 tgaagcaacg ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct
     5401 ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga
     5461 tgtcctgcaa tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt
     5521 ttcacaactg accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga
     5581 cggggcgcgt aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt
     5641 cgatgaagat caggctcgcc acgcacggat tacccgcctg cccggtgagc attatgagca
     5701 ttcgagggag aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa
     5761 cgcattctgc cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg
     5821 ctgcgccgca acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac
     5881 atgtctgact taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca
     5941 ctggcaccgg tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa
     6001 gcggcggacg cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa
     6061 gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc
     6121 ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc
     6181 accgtggata tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc
     6241 ccaaatgcgg caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat
     6301 gtgggcctga acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg
     6361 accggactgg aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg
     6421 aaagaccttg agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc
     6481 gatccggaag atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac
     6541 cacggtggcc gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca
     6601 gatgcggtga aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat
     6661 gtcgtgcgta tgattgcgct cggtgccgac accgtacttc tgggtcgtgc tttcctgtat
     6721 gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgagaaagag
     6781 atgaaagtgg caatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg
     6841 ctggtgcagg ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat
     6901 gcggcatagt cgtttgagcc ccctcaccct aaccctctcc ctcagggagt ggggaccgtt
     6961 cggcgctgta tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc
     7021 cctccggggt gaggggaaaa ggccgcactg ctcaccaatt ttctgctatc ctgcccccgc
     7081 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     7141 gcaatatcat ccgtctttgc gctaataccg gctttcgtct gcatatcatc gaaccgatgg
     7201 gatttgcctg ggacgataag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     7261 ccgttacgcg tcatcatgac tttcgcgcat tcctcgaagc agaaaatccc cagcgcctgt
     7321 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aaactatcag gatggcgatt
     7381 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
     7441 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaac ctgtccaatg
     7501 cggtgtcagt tgtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
     7561 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
     7621 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
     7681 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
     7741 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc
     7801 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
     7861 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
     7921 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
     7981 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
     8041 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
     8101 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
     8161 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
     8221 gtcagcggcg taggcttctt ccaccacttc acggatagca atagcaggca taattggcga
     8281 tgacagcttg ttcgccagca tgtagctcag tgcactacca aggttttcgt gcttgagtag
     8341 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
     8401 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
     8461 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
     8521 gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taacgtcaat gctgcctcgc
     8581 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttcaacgc
     8641 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
     8701 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc
     8761 ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc ccccagcccc gccatgccca
     8821 taaaggtggc aggatcggca cccagcgccg cgccaagacg cgacatttca gccagcccac
     8881 gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcggac atccccgcac
     8941 caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa tccggattgc
     9001 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
     9061 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
     9121 ttgggccaga gataaccgcc agcggaattt gatcgcctaa cgcctcgcgg gccacgtcct
     9181 gtaacagacg cccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
     9241 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
     9301 cgagaatatt acggctggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
     9361 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
     9421 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtgatgg
     9481 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
     9541 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
     9601 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
     9661 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
     9721 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
     9781 acacaggaac gcggtttctt cgcccaggga ggccgttacg gtaacacgca gtaccacttc
     9841 gtaaacgtca tctgccagtt gagtagatgc cgtatccaga tcaagtttaa cttctggctg
     9901 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagaaatat ccttggtata
     9961 aatacgttgg atctggaaag tcatttcggt gttgttttgt tctgacatgt gtagaaaacc
    10021 cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc
    10081 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    10141 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca
    10201 cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt gctgctcagc
    10261 agtgcttttg cacgatggca atacgggcag gtttctttag tatagatttc aacattggcc
    10321 ataaattagc tcctgttttt taccctgaaa tttcatgttg cagggcggca gcaaaatgga
    10381 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    10441 atcatgaaag acgacaggta aattatttgc cgcgcaccaa aggcaagttt tcgccagccc
    10501 agccagcgac gccttctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcat
    10561 ttgcaggctc ctggcactgc aggccagaac cgtctaccac gataaccggt ttgtctttgt
    10621 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac
    10681 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    10741 ctttgttgat cagacgcgta gcttcaccac gactaatcac cttcactttc gaggtcaggc
    10801 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    10861 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    10921 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    10981 actggaatgc aattttgcgg ggcgcgacga aaaaaaagcg taaatctggc atcggcttgc
    11041 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    11101 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    11161 tggtactggt gattctggat ggctatggct atcgcgaaga acagcaggat aacgccattt
    11221 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    11281 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    11341 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    11401 tcaaagatcg cgctttcttt gctaatccag tgctgactgg cgcggtagat aaagcgaaaa
    11461 acgcaggtaa agcagtacac attatgggtc tgctctccgt aggcggcgta cacagccacg
    11521 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    11581 tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa
    11641 aatttgaaga gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    11701 actacgcgat ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga
    11761 ctctggcgca gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg
    11821 ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    11881 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    11941 gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg
    12001 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    12061 acccacccgc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    12121 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    12181 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    12241 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggcaatca
    12301 aaagcggcaa atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca
    12361 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    12421 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    12481 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    12541 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg
    12601 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    12661 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    12721 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    12781 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    12841 ctattcaggc cgatatcgcc gcaaaagagc gcgcggtacg ccaaaagcaa caacaacgcg
    12901 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    12961 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    13021 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcgcaactgg
    13081 atgccgcatt ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa
    13141 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    13201 ccattgccca gttgaagcaa acgcgtgaag aagtcgccat gcagcgtgct gaactggaag
    13261 agaaacaaag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga
    13321 ctcaggcgct gaacgagcgt aaaaagacgc tggcagggct ggagtcttcc atccagcaag
    13381 gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg tctgcgtaac agcattgccc
    13441 gtgcggaagc cgcggcgaaa gcgcgtgcag aacgagaagc acgtgaggcc caggcggttc
    13501 gcgaccgcca gaaagaagcg acgcgtaaag gcaccaccta caaaccgact gaaagcgaaa
    13561 aatcgctgat gtcccgtacc ggtggtctgg gcgcgccgcg tggtcaggca ttctggccgg
    13621 ttcgcgggcc gacgctgcat cgctatggcg aacagctaca gggtgaatta cgctggaaag
    13681 ggatggttat cggtgcctct gaaggtactg aagttaaagc gattgccgac ggtcgggtga
    13741 ttctggctga ctggctgcaa ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg
    13801 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    13861 cgggccagcc aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    13921 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    13981 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    14041 tacttgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    14101 accaggtgct ggcgatgccc tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    14161 ccagagagat ggcgaccaaa gcgcataaca gcgggcacga agtgttgatt catctcccga
    14221 tggcaccact gagtaaacag ccgctggaga aaaatacgct gcgcccggag atgagcagcg
    14281 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    14341 acaaccacat gggtagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    14401 cactggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaggcga
    14461 tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt
    14521 cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca
    14581 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    14641 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    14701 cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt
    14761 tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    14821 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    14881 agtggcaagg ctggggcaaa cagcccgaag cggcgaagtt taacgctagc gcaaattaaa
    14941 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    15001 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    15061 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    15121 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    15181 ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    15241 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    15301 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcgtt
    15361 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    15421 gttattttta acaataacat cacggcgata aacccccatc cagacaacgt gagtccaacg
    15481 gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacgccgg ttgagcgaag
    15541 gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt
    15601 gcactgcgcc acgtcgaggt catcctctaa cgccatggtc atcagcgttt cgtacatggt
    15661 gggatagacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg ccacctcgat
    15721 cccacgatta cgcgctaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    15781 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    15841 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    15901 atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc tgttcatcat
    15961 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    16021 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    16081 gagagcagct atataagaga tcagcgcaat tacgatatct ttattacact gacaggattt
    16141 tacatattta acggattatc ctcaggcaaa agggctggaa taccagccct tgttcgtgtt
    16201 aatcccaact cagaattact ttcccggact ggcccgaacg catagcgtca aagcccttct
    16261 ggaaatcatc gatagagaaa cgatgggtaa tgatcggcga gagatccaga cctgactgaa
    16321 tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaata cctttaatga
    16381 acaagccttt aaagattact ttggtccagt cgatagacat atcggacggt ggaataccca
    16441 gcatcgcaat gcgaccgccg tggttcatgg tgtcgagcat ggtacgaaac gctggcggcg
    16501 cgccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttct gccatcacat
    16561 cattaaggtt ttctttagcg acgttgaccg cacgggtgat acccattttg cgcgccagct
    16621 caaggcggta ttcgttaaca tcggtgatca ccacattgcg tgcaccaacg tgtttcgcca
    16681 ccgccgcagc cataatacct atcggacctg caccagaaac cagcacatct tcgcccacca
    16741 gatcgaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccaggt
    16801 cgtcggagat attgtcgggg attttgaaag cgttgaacgc cggaatcacc agatattcgg
    16861 cgaagcagcc aggacggtta acaccgacac caatggtgtt gcggcacaga tgggtacgac
    16921 cgccacgaca gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa acgcgatcac
    16981 cgattttgaa gcctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    17041 ctacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    17101 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    17161 ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt
    17221 tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    17281 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    17341 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatgccctct ttttgcagct
    17401 cacgggcaaa tttctgcgcc actactgcat cgccaagcat gaccggaata atggcatgat
    17461 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactgg cgcgcgttcg
    17521 cccacagacg gtcacgcagt tcgctgcccg cttcgaccat ctccagtact ttgatggacg
    17581 cggcaacaat ggccggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca
    17641 gccactcaac cacctctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt
    17701 taccaagcgt accggtgata atatcgaccc ggcccatcac atcgcagtat tcatgggaac
    17761 cacgaccatt ttcaccgaca aaaccgaccg cgtgggagtc gtctaccatc accagggcat
    17821 cgtatttatc tgccagatcg caaacgccct tcagattggc aatcatgccg tccatcgaga
    17881 atacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    17941 gagcttcaag ctcctgcata tcgttgttgg catagcgata gcgtttagct ttacacagac
    18001 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    18061 ccagcagcgt ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    18121 cttccatccc caggaaggcc gccagttttt gttcaagctc tttatggctg tcctgagtgc
    18181 cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgccg
    18241 ccgcaatcag atcaggatga ttcgccagcc cgagatagtt gttggcacaa aagttaatga
    18301 cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgtaata atgcgctctt
    18361 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    18421 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    18481 ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata acgtgtaact
    18541 tgatgtctct caacacctac agatagtaga aaaactgtac agttttggct gggcataatg
    18601 atgattgatt ttactgcatt gatataaaaa gaaaaaatga tgccaaagca tcatttttcc
    18661 tatctcagtt ggtatcaaaa cgctacccac taatggaggg aaacctattt tcttgaaagg
    18721 aaaattctca gcgcctgcat ttttgcccct aaagtgttac tcctgaagca gcgaaacaaa
    18781 tcgaaccatt ttcctctccc cagatagttt aggttgaaga gatcggggcg tttgtaatag
    18841 cacatgacaa aaatgccctg gtcatcatcc acaatgttat tatttagtgt tattttttga
    18901 ctttccagaa ctaatttata gaattctttc catttatgct gtgaaccaac aattgcgccc
    18961 ccaataatat atacatgatt gccaatcatg aaatcaaaaa cctgttgttg agatgtgaca
    19021 gtaagccctt tttttatagt gaaaagatgc atcttacttt catcaaatgg gaaatcccaa
    19081 attttcaatc ctcgagttac atttggctta cggcaatagc caaaatctat ccacgcaaca
    19141 agaggcgttt tcactaagcc catgttgata gctttattta caaaataggc cttaaggtta
    19201 cataccaaaa catactctgg tgaccagtac tctgggtttt ttaattgtcg aggctcaagt
    19261 ctatttgtaa acgactcatc tttttgaatt ttttcgatac gacttctgat a
//
LOCUS       DAPHDX010000081        19231 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPHDX010000081 DAPHDX010000000
VERSION     DAPHDX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.081"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(194..841)
                     /gene="ychE"
                     /locus_tag="RH058_002899"
     CDS             complement(194..841)
                     /gene="ychE"
                     /locus_tag="RH058_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV3138463.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1318..3993
                     /gene="adhE"
                     /locus_tag="RH058_002900"
                     /gene_synonym="adhC"
     CDS             1318..3993
                     /gene="adhE"
                     /locus_tag="RH058_002900"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV3138464.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4117..5017
                     /locus_tag="RH058_002901"
                     /pseudo
     CDS             4117..5017
                     /locus_tag="RH058_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5299..5916)
                     /gene="tdk"
                     /locus_tag="RH058_002902"
     CDS             complement(5299..5916)
                     /gene="tdk"
                     /locus_tag="RH058_002902"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV3138465.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6522..6935
                     /gene="hns"
                     /locus_tag="RH058_002903"
     CDS             6522..6935
                     /gene="hns"
                     /locus_tag="RH058_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV3138466.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7079..7987)
                     /gene="galU"
                     /locus_tag="RH058_002904"
     CDS             complement(7079..7987)
                     /gene="galU"
                     /locus_tag="RH058_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV3138467.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8189..9202)
                     /gene="rssB"
                     /locus_tag="RH058_002905"
     CDS             complement(8189..9202)
                     /gene="rssB"
                     /locus_tag="RH058_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV3138468.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9294..10199)
                     /gene="rssA"
                     /locus_tag="RH058_002906"
     CDS             complement(9294..10199)
                     /gene="rssA"
                     /locus_tag="RH058_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV3138469.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10312..10770
                     /locus_tag="RH058_002907"
     CDS             10312..10770
                     /locus_tag="RH058_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV3138470.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10820..11662
                     /gene="purU"
                     /locus_tag="RH058_002908"
     CDS             10820..11662
                     /gene="purU"
                     /locus_tag="RH058_002908"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV3138471.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11822..11906
                     /locus_tag="RH058_002909"
     tRNA            11822..11906
                     /locus_tag="RH058_002909"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11856..11858,aa:Tyr,seq:gta)
     gene            11941..12025
                     /locus_tag="RH058_002910"
     tRNA            11941..12025
                     /locus_tag="RH058_002910"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11975..11977,aa:Tyr,seq:gta)
     gene            12072..12203
                     /locus_tag="RH058_002911"
     ncRNA           12072..12203
                     /ncRNA_class="other"
                     /locus_tag="RH058_002911"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12387..13064)
                     /gene="narI"
                     /locus_tag="RH058_002912"
     CDS             complement(12387..13064)
                     /gene="narI"
                     /locus_tag="RH058_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV3138472.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(13064..13774)
                     /gene="narJ"
                     /locus_tag="RH058_002913"
     CDS             complement(13064..13774)
                     /gene="narJ"
                     /locus_tag="RH058_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV3138473.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13771..15309)
                     /gene="narH"
                     /locus_tag="RH058_002914"
     CDS             complement(13771..15309)
                     /gene="narH"
                     /locus_tag="RH058_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV3138474.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15306..19049)
                     /locus_tag="RH058_002915"
     CDS             complement(15306..19049)
                     /locus_tag="RH058_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV3138475.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYCGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLVFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4853 a         5049 c         4603 g         4726 t
ORIGIN      
        1 caagcccagg attatgatgt ggcgaaaata acatgatggt gaggagaagc agttaaggaa
       61 atccttataa ctaactgtaa tttgagaaga atatttttac ctattccttt tgcttttgat
      121 aactgcgcgg gaacaaagta aaaataatga cgaagtaaac ggagcatggc agctccgttt
      181 catttgaaag gaattaatta agcaggccgg ggaaaatccc cttaatacca gtaacgataa
      241 attcaatccc caatgccatc agcaatagcc ccataatacg cgtaatcacg ttgatgccgg
      301 tctggcgtaa aacccgtacc agccacggtg ccatgcggaa caatccccag caacataaag
      361 cgaacaatgc aatagccaca aagaaaccaa acagatagct aatgctgtga taacgcgtac
      421 cccagacgat ggtagaactg atcgcccctg gccccgccat caacggcaac gccaatggca
      481 ccacaccaat gctttcacgt accgcggttt ctgatttttc ttgcttgttc tgtttatcct
      541 cgccaagctt gccgctgatc atcgacatcg ctatcgtcac caccaggatg cccccggcga
      601 tacggaacga atcaattgat ataccaaaaa gttgcagaat cgtgtcgccg agaaaaagcg
      661 agatccacaa gataatggcc acagacaggt tggctgtaag gttagttttg tttcgcgctg
      721 ccgctgtctg ataactggtc atgctgataa agacgggaat aatccctacc gggttgacca
      781 gcgcaaataa cccgatgaaa aatttgaagt aaacgggaaa atcaaaaaag gtctgaatca
      841 cggttagctc cgaagcaaaa gccggataat gttagccata aataaggttg aaaagacgcg
      901 ctgacaatac gccttttgac agcatttttc acctcctaac tacttaaaat tgctatcatt
      961 cgttattgtt atctagttgt gcaaaacatg ctaatgtagc caccaaatca tactacaatt
     1021 tattaactgt tagctataat ggcgaaaagc gatgctgaaa ggtgtcagct ttgcaaaaat
     1081 ttgatttgga tcacgtaatc agtacccaga agtgagtaat cttgcttacg ccacctggaa
     1141 gtgacgcatt agagataata actctaatgt ttaaactctt ttagtaaatc acagtgagtg
     1201 tgagcgcgag taagcttttg attttcatag gttaagcaaa tcatcaccgc actgactata
     1261 ctctcgtatt cgagcagatg atttactaaa aaagtttaac attatcagga gagcattatg
     1321 gctgttacta atgtcgctga acttaacgca ctcgtagagc gtgtaaaaaa agcccagcgt
     1381 gaatatgcca gtttcactca agagcaagta gacaaaatct tccgcgccgc cgctctggct
     1441 gctgcagatg ctcgaatccc actcgcgaaa atggccgttg ccgaatccgg catgggtatc
     1501 gtcgaagata aagtgatcaa aaaccacttt gcttctgaat atatctacaa cgcctataaa
     1561 gatgaaaaaa cctgtggtgt tctgtctgaa gacgacactt ttggtaccat cactatcgct
     1621 gaaccaatcg gtattatttg cggtatcgtt ccgaccacta acccgacttc aactgctatc
     1681 ttcaaatcgc tgatcagtct gaagacccgt aacgccatta tcttctcccc gcacccgcgt
     1741 gcaaaagatg ccaccaacaa agcggctgat atcgttctgc aggctgctat cgctgccggt
     1801 gctccgaaag atctgatcgg ctggatcgat caaccttctg ttgaactgtc taacgcactg
     1861 atgcaccacc cagacatcaa cctgatcctc gcgactggtg gtccgggcat ggttaaagcc
     1921 gcatacagct ccggtaaacc agctatcggt gtaggcgcgg gcaacactcc agttgttatc
     1981 gatgaaactg ctgatatcaa acgtgcagtt gcatctgtac tgatgtccaa aaccttcgac
     2041 aacggcgtaa tctgtgcttc tgaacagtct gttgttgttg ttgactctgt ttatgacgct
     2101 gtacgtgaac gttttgcaac ccacggcggc tatctgttgc agggtaaaga gctgaaagct
     2161 gttcaggatg ttatcctgaa aaacggtgcg ctgaacgcgg ctatcgttgg tcagccagcc
     2221 tataaaattg ctgaactggc aggcttctct gtaccagaaa acaccaagat tctgatcggt
     2281 gaagtgaccg ttgttgatga aagcgaaccg ttcgcacatg aaaaactgtc cccgactctg
     2341 gcaatgtacc gcgctaaaga tttcgaagac gcggtagaaa aagcagagaa actggttgct
     2401 atgggcggta tcggtcatac ctcttgcctg tacactgacc aggataacca accggctcgc
     2461 gtttcttact tcggtcagaa aatgaaaacg gctcgtatcc tgattaacac cccagcgtct
     2521 cagggtggta tcggtgacct gtataacttc aaactcgcac cttccctgac tctgggttgt
     2581 ggttcttggg gtggtaactc catctctgaa aacgttggtc cgaaacacct gatcaacaag
     2641 aaaaccgttg ctaagcgagc tgaaaacatg ttgtggcaca aacttccgaa atctatctac
     2701 ttccgccgtg gctccctgcc aatcgcgctg gatgaagtga ttactgatgg ccacaaacgt
     2761 gcgctcatcg tgactgaccg cttcctgttc aacaatggtt atgctgatca gatcacttcc
     2821 gtactgaaag cagcaggcgt tgaaactgaa gtcttcttcg aagtagaagc ggacccgacc
     2881 ctgagcatcg ttcgtaaagg tgcagaactg gcaaactcct tcaaaccaga cgtgattatc
     2941 gcgctgggtg gtggttcccc gatggacgcc gcgaagatca tgtgggttat gtacgaacat
     3001 ccggaaactc acttcgaaga gctggcgctg cgctttatgg atatccgtaa acgtatctac
     3061 aagttcccga aaatgggcgt gaaagcgaaa atgatcgctg tcaccaccac ttctggtaca
     3121 ggttctgaag tcactccgtt tgcggttgta actgacgacg ctactggtca gaaatatccg
     3181 ctggcagact atgcgctgac tccggatatg gcgattgtcg acgccaacct ggttatggac
     3241 atgccgaagt ccctgtgtgc tttcggtggt ctggacgcag taactcacgc catggaagct
     3301 tatgtttctg tactggcatc tgagttctct gatggtcagg ctctgcaggc actgaaactg
     3361 ctgaaagaat atctgccagc gtcctaccac gaagggtcta aaaatccggt agcgcgtgaa
     3421 cgtgttcaca gtgcagcgac tatcgcgggt atcgcgtttg cgaacgcctt cctgggtgta
     3481 tgtcactcaa tggcgcacaa actgggttcc cagttccata ttccgcacgg tctggcaaac
     3541 gccctgctga tttgtaacgt tattcgctac aatgcgaacg acaacccgac caagcagact
     3601 gcattcagcc agtatgaccg tccgcaggct cgccgtcgtt atgctgaaat tgccgaccac
     3661 ctgggtctga gcgcaccggg cgaccgtact gctgctaaga tcgagaaact gctggcatgg
     3721 ctggaaacgc tgaaagctga actgggtatt ccgaaatcta tccgtgaagc tggcgttcag
     3781 gaagcagact tcctggcgaa cgtggataaa ctgtctgaag atgcattcga tgaccagtgc
     3841 accggcgcta acccgcgtta cccgctgatc tccgagctga aacagattct gctggatacc
     3901 tactacggtc gtgattatgt agaaggtgaa actgcagcga agaaagaagc tgctccggct
     3961 aaagctgaga aaaaagcgaa aaaatccgct taatcagtag cgctgtctgg caacataaac
     4021 ggccccttct gggcaatgcc gatcagttaa ggattagttg accgatcctt aaactgaggc
     4081 actataacgg cttccacaac agggagccgt tttcttatgc cacttctcaa tgatctgctc
     4141 gatttcagtg accatccgct tatgcctccg ccctctgcac aactatttgc agaacacctt
     4201 cccaccgagt ggatacaaca ctgcctgacg ctttctgctc atgcgaccgt tcgccgccgt
     4261 cgtttaccgg gggacatggt tatctggatg gtggtgcaat gagccaatta ccgatgttgt
     4321 tcgccgtctg aacctgagcg cggatggcga agcggggatg aacctgctgg cccgcagcgc
     4381 tgtcacccag gcgcgtcagc gcgtgggggc cgccccagtg gaatggctct tccgccagac
     4441 cgcacagaca ggggcgcgga acgttacctg aaggatgact ggcacggcct gcaacttttt
     4501 gccattgatg gcgcacagtt caggacacct gactgataaa cccgagctgc gtgaatatta
     4561 tgggtctgcc aacacatcca ctaagcggca gaacgcctac ccggttatgc gtctggtggc
     4621 cttaatgaac ctgggaagtc acattctgct gaatgccgtg accgcacctt accggcagag
     4681 cgaaactgtg ctggcccact ccatgctcgc caccatcccg gataactcta ttacgttgtt
     4741 tgataaactt ttttacagcg aagacctgct gctgacgctg aaccagaagg gttgtaaccg
     4801 ccactggcta ctgcctgcgt ggaagaatat cgcatcagaa atgatagagc tgggaaacac
     4861 ggcgtcacca gggaccatcc cgaaacgact ggagcatctt cagggtgctc tggaagtggt
     4921 gttcataaca aaacgccccc gaccatcaag gccgaggtcg gttaagatat caaaaacccg
     4981 ttatcctgtg aaacatagtg ccgctcctct taagtgaacg gcattgccct tctggggccg
     5041 tattttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc ttcatcatat
     5101 ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc acaagtcaac
     5161 gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc gagaagggta
     5221 tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct gctcaccctg
     5281 tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg ttaatgagcc
     5341 gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt cattaccacc
     5401 aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac gcagcaccat
     5461 gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt cggaccatgc
     5521 cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta aaccataaca
     5581 aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt gttgtctggt
     5641 taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat gttccgcacg
     5701 aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg acaaacctat
     5761 acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat atacgacagt
     5821 gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag acttacccgc
     5881 attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct caatgatatg
     5941 aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac agtatgcaca
     6001 gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga gctatcatta
     6061 caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca caatctttaa
     6121 cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat actaagtcca
     6181 tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt ctcgtaaaca
     6241 caactaatac agaagactga aaggtcgtca gcctacgata atctccccat aaaatgtgac
     6301 atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat agggctatat
     6361 gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca aaataaagaa
     6421 caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc tctattatta
     6481 cctcaacaaa ccacccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
     6541 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
     6601 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
     6661 ctgctgctga agttgaagag cgcactcgta agctgcagca atatcgcgaa atgctgatcg
     6721 ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca
     6781 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
     6841 aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
     6901 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
     6961 aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
     7021 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
     7081 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
     7141 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
     7201 tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt
     7261 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
     7321 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg
     7381 gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     7441 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     7501 ccgtttcatc aaagcggcgg atcatctctg ccaggttatc ctgtgacaaa tcggattcat
     7561 attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
     7621 gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact tgcataatag
     7681 tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     7741 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     7801 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     7861 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     7921 acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
     7981 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     8041 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gttagcataa
     8101 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa
     8161 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
     8221 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     8281 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
     8341 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     8401 aatagccaac taatagcgga aactgccccg gcagattggc ctgacgaagt aaatggttta
     8461 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     8521 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
     8581 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     8641 gcaccaggcc gggtttgtcc gcggcaacca attgacgata attaacccgg caatgggaaa
     8701 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
     8761 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
     8821 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
     8881 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag
     8941 tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt
     9001 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     9061 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     9121 aaaaccatga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     9181 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg
     9241 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     9301 ttggtgcgta ccaacggcaa aagttcgtcc attttccttt ccactgccag ctgtccggcc
     9361 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     9421 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccagcacc
     9481 tggatagaag tggtcataat ctctgttgcc gttggcgctg tcaccgcccg acgtgtcgtt
     9541 atgctgccta acctttcttt cagacgcgca tgccacggca gagaatcacc attctcgcta
     9601 ttttcttcac tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc atcgtgctgc
     9661 aggtcaaccg ctatcacaat atcagccccc aatgcacgcg tgagggaaat aggaattggg
     9721 ttaacgactg ctccatcaac cagccagtag ccgttatgtg caacaggtgc catgagtcct
     9781 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
     9841 cgtcccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct
     9901 gtttccggca ttatttcgcg gtattgattg aagacacgct cgccgcgcag taacccaccg
     9961 cgctgccagg agagatccat caggcgtaaa acatcccaat aactgaaaga ggtcacccaa
    10021 tcttccagcg cagataatcg atcgcatgca taggcagcgc ccaccagcga accaattgaa
    10081 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
    10141 tgcgaccaac ctctcgccgc gccagatccc aacgccagcc ctatttttat ctttctcatt
    10201 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
    10261 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
    10321 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
    10381 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
    10441 caagacgcag attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt
    10501 gccgaattga tggccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
    10561 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
    10621 ggtaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
    10681 tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt
    10741 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
    10801 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
    10861 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
    10921 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
    10981 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
    11041 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
    11101 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggcctgg atgtcgaaat
    11161 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc
    11221 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
    11281 cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
    11341 gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc
    11401 tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg
    11461 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
    11521 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
    11581 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggca cagcgcgtct ttgtttacgg
    11641 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
    11701 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
    11761 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
    11821 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
    11881 aggttcgaat ccttccccca ccaccatctc aagtattacc tcaaaatttg aattacccct
    11941 ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa
    12001 ggttcgaatc cttcccccac caccatcact ttcaaaagtc cgtgaactct caaacgaatc
    12061 cgcgattaaa tattctgccc atgcggggaa ggatgagaag cctcgaccaa ggttcgactc
    12121 gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc gcgcagcgtt
    12181 gagtaatcct tcccccacca ccatcacttc cttaaataat caaaacctgt tactccgagc
    12241 taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct gtaggtcgga
    12301 taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac cccgccgaag
    12361 cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg gtacttacgt
    12421 gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg gaacagcagg
    12481 aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc atcgaggtgt
    12541 tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag tttcatcatc
    12601 tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag cgcgcactgg
    12661 ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg cacgcgtggg
    12721 ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac gccgctggca
    12781 ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc ttcatacatc
    12841 cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat accgatatgg
    12901 aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga cgccgcgcgc
    12961 caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac cgccccggct
    13021 atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc tgtcctccgg
    13081 tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga
    13141 ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct tcccagacag
    13201 catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc gccactttgt
    13261 cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg gcataacggc
    13321 tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata tctttcaaac
    13381 cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc ggcagatggt
    13441 ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca
    13501 ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc aacagtgacg
    13561 tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc ggatccatcg
    13621 ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac agatttttcg
    13681 acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca tccggatatt
    13741 caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt tgctggtcac
    13801 atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg aaccgtggca
    13861 gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc gtgccagttc
    13921 acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca gataacggta
    13981 catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg tatcaacttt
    14041 accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt tcagtgcgcg
    14101 cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta ccggaatacg
    14161 caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt ctgccgcaga
    14221 ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat attctggatg
    14281 cagcggcagc gccagtttcc attccattgc cattttataa accggcgact gctgtgcggc
    14341 ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt
    14401 cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct cggtgctggc
    14461 tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga tacgaccgac
    14521 acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc agaagatgca
    14581 cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc atccggtgat
    14641 gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat cttcttcacg
    14701 cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca
    14761 caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg ccttctggat
    14821 gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt catcttccca
    14881 gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac gcggacgggc
    14941 aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt caaaatcgaa
    15001 cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt tacccagcaa
    15061 catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga tccagccgcc
    15121 tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct tggtttccac
    15181 gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt tacaggtaac
    15241 tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga cttgtgaacg
    15301 aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg
    15361 ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt gccgtagtag
    15421 ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt cggcgtgata
    15481 cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc aggcaggtta
    15541 acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg ctggctgaca
    15601 acagcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc gttatcggcg
    15661 atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc gcgacctaaa
    15721 gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg gtgcggcgtc
    15781 aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac ttctttcacc
    15841 gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc acgcatccac
    15901 tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat cagctcgtga
    15961 acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg
    16021 ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt ctcgtcttct
    16081 ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc agcccaggct
    16141 ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc tgccgcatca
    16201 attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt gtagttgagc
    16261 ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc gccattaccg
    16321 attttctcca tcagcgggcc gatagaggta aagcgttcgt aagtcgccgg ataatcgcgc
    16381 tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc gccttttttc
    16441 cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg gataggcagc
    16501 gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa tttcttcgcg
    16561 atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac cgccgcagac
    16621 agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc gtaccaggtc
    16681 gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa gtccagcgtc
    16741 accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc ttccggcttc
    16801 acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc cagcaggtac
    16861 ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg ccagatgaac
    16921 aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg gatggaaccc
    16981 tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc tgcttcgcca
    17041 gcgatagtca gcgggttagt gcctaactgc ggcgcagacg gcagccagcc catgcgttct
    17101 gcacggacgt taaagtcgat caggtgtccg gtataacggg atttatccgc catcggcgac
    17161 agcagttctt ccgcagtcac ggtttcataa cgccactggc tggagtgatt atagaaataa
    17221 gaagtgctgt tcatatgacg cgccggacgc tgccagtcaa gggcaaacac cagcggctgc
    17281 cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc gcccccgctc
    17341 tgaccgacac agccgcagaa aatcagcatg ttgatcagac cgcgatagtt catatcgagg
    17401 tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg cgttttatca
    17461 gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac gccggtgatc
    17521 tgctcagccc aggccggggt gtaagctttc acatcgtcat agctggttgc acagttaacg
    17581 tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac ggtggtcacc
    17641 agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg
    17701 ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccacagta cgggaagcct
    17761 acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt ttcttcgcca
    17821 gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg gaagccaata
    17881 gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt ccattccgga
    17941 ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg acctgcagcg
    18001 tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta gcgacgcaca
    18061 tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat tacgtggccc
    18121 atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag atcgcacagt
    18181 ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtcccttt gtaacgcact
    18241 tcagtaaaga agtgagcatc cggggtacgc gtctgcggta cgtttgaccc ccaggcgatg
    18301 atgtagctgg agttgtacca gtcagcagat tccggtacgt cagtttgctc gccccaggtt
    18361 tgcggagacg caggaggcaa gtcgcagtac cagtcgtaga agcttaagca agtaccgcca
    18421 atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc cggaattggc
    18481 gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg
    18541 atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc acgcgcctgc
    18601 ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc aaccggatcg
    18661 ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat catcgggtat
    18721 ttcaggcggt tggcgctgta aagataccag gagtagctgg caccgcgcgg gcagccgcga
    18781 ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg ggtttcccag
    18841 gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca gtttaccccg
    18901 tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc ctcccagtca
    18961 cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt ctgtttgaag
    19021 tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc tgtcggccct
    19081 ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa
    19141 ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag ataccccttt
    19201 aggataatta acagccacca cataacagac t
//
LOCUS       DAPHDX010000082        18831 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPHDX010000082 DAPHDX010000000
VERSION     DAPHDX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.082"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..565
                     /gene="alaE"
                     /locus_tag="RH058_002916"
     CDS             116..565
                     /gene="alaE"
                     /locus_tag="RH058_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV3138476.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(602..946)
                     /locus_tag="RH058_002917"
     CDS             complement(602..946)
                     /locus_tag="RH058_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV3138477.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1098..1427
                     /locus_tag="RH058_002918"
     CDS             1098..1427
                     /locus_tag="RH058_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV3138478.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1675..1920
                     /gene="nrdH"
                     /locus_tag="RH058_002919"
     CDS             1675..1920
                     /gene="nrdH"
                     /locus_tag="RH058_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV3138479.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1917..2327
                     /gene="nrdI"
                     /locus_tag="RH058_002920"
     CDS             1917..2327
                     /gene="nrdI"
                     /locus_tag="RH058_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV3138480.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2300..4444
                     /gene="nrdE"
                     /locus_tag="RH058_002921"
     CDS             2300..4444
                     /gene="nrdE"
                     /locus_tag="RH058_002921"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV3138481.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4454..5413
                     /gene="nrdF"
                     /locus_tag="RH058_002922"
     CDS             4454..5413
                     /gene="nrdF"
                     /locus_tag="RH058_002922"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV3138482.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLDYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5768..6970
                     /gene="proV"
                     /locus_tag="RH058_002923"
     CDS             5768..6970
                     /gene="proV"
                     /locus_tag="RH058_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV3138483.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6963..8027
                     /gene="proW"
                     /locus_tag="RH058_002924"
     CDS             6963..8027
                     /gene="proW"
                     /locus_tag="RH058_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV3138484.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8084..9076
                     /gene="proX"
                     /locus_tag="RH058_002925"
     CDS             8084..9076
                     /gene="proX"
                     /locus_tag="RH058_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV3138485.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9367..10551
                     /locus_tag="RH058_002926"
     CDS             9367..10551
                     /locus_tag="RH058_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138486.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10675..11412
                     /gene="ygaZ"
                     /locus_tag="RH058_002927"
     CDS             10675..11412
                     /gene="ygaZ"
                     /locus_tag="RH058_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV3138487.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11402..11737
                     /gene="ygaH"
                     /locus_tag="RH058_002928"
     CDS             11402..11737
                     /gene="ygaH"
                     /locus_tag="RH058_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV3138488.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11828..12358
                     /gene="emrR"
                     /locus_tag="RH058_002929"
     CDS             11828..12358
                     /gene="emrR"
                     /locus_tag="RH058_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV3138489.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12485..13657
                     /gene="emrA"
                     /locus_tag="RH058_002930"
     CDS             12485..13657
                     /gene="emrA"
                     /locus_tag="RH058_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV3138490.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13674..15212
                     /gene="emrB"
                     /locus_tag="RH058_002931"
     CDS             13674..15212
                     /gene="emrB"
                     /locus_tag="RH058_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV3138491.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15276..15791)
                     /gene="luxS"
                     /locus_tag="RH058_002932"
     CDS             complement(15276..15791)
                     /gene="luxS"
                     /locus_tag="RH058_002932"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV3138492.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15941..17497)
                     /gene="gshA"
                     /locus_tag="RH058_002933"
     CDS             complement(15941..17497)
                     /gene="gshA"
                     /locus_tag="RH058_002933"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV3138493.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17570..17998)
                     /locus_tag="RH058_002934"
     CDS             complement(17570..17998)
                     /locus_tag="RH058_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3138494.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17995..18561)
                     /gene="yqaB"
                     /locus_tag="RH058_002935"
     CDS             complement(17995..18561)
                     /gene="yqaB"
                     /locus_tag="RH058_002935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV3138495.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4454 a         4878 c         4772 g         4727 t
ORIGIN      
        1 ctaattgaat agtcatagcc agcgatatac gctatgcgaa aatgcagatg acaatgagat
       61 ccactgcttt catctccatt aacatcccat tacgctttta ttaaggagca ttagcatgtt
      121 ctcaccgcag tcacgcttgc gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg
      181 ttctgtcgtg aacatgtgta ttgaagtttt cctctccgga atgagcttcg aacagtcttt
      241 ttattccaga ttggtagcga ttccggtgaa catcttaatt gcatggccat acggtatgta
      301 ccgcgatctg tttatgcgcg cggcacgcaa agttagcccg tcgggctgga taaaaaatct
      361 ggctgatatc ctggcttatg tgacgttcca gtcaccggtg tatgtggcga tcttgttagt
      421 ggtgggcgca gactggcatc agattatggc ggcggtcagt tcaaacatcg ttgtttcgat
      481 gttgatgggg gcggtttatg gctacttcct cgattattgc cgccgactgt ttaaagtcag
      541 ccgttaccag caggtaaaag cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc
      601 tttaactggc ttcgccaaat aaaccattca aataacgttc aagcgcaaca cgcgaactga
      661 agccatgcgg gataccgtaa tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct
      721 ggtaatgatc gcaagtttta atatcggaag cgggttcagc cagtgttgaa ctgcgttctt
      781 ttaatgttgg atgaattacc agagcggtac gtcccattcg cgcttcgcga ttaacataga
      841 cataattttc cccacggcgg taaccatacg ctttctgggt taccacatcg acagtaaaac
      901 cgactttttc aagtacgcgc gccacctcgt ctggtcgtaa atacatattt gatcctcgtt
      961 attatcaacc gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc
     1021 ataaagccgc gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa
     1081 ttctaatggg ggatcatatg tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc
     1141 aggatatcca gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttaaagtcct
     1201 ggggcagcga cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcattgctga
     1261 aagaaaccag ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgca cgcgatgccg
     1321 ttggctgcgc ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg
     1381 cggtaggtat ttttatcggc gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata
     1441 ataaatacgc gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc
     1501 acaaaaatcc ccctaccccg tcacgctcat atccagggta atttcgacca ctatttgcta
     1561 tatattgtgt ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga
     1621 caacccgacg cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc
     1681 attactattt acactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa
     1741 aaccggggct ttgattttga aatgattaat gtcgatcgcg ttcctgaagc ggcggaagcg
     1801 ctgcgtgctc agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct
     1861 ggtttccgcc cggacatgat caatcgtctg catccagcgc cacacgcggc cagtgcatga
     1921 gccagctcgt ctacttttcc agtagctccg aaaatacgca gcgttttatc gaacgtttag
     1981 gactgcccgc ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt
     2041 acatcctgat cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg
     2101 taattcgctt tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttcgg
     2161 gtaatcgcaa ctttggtgag gcgtatggcc gtgccggaga tgtgattgct cagaaatgcg
     2221 gcgtgccgtg gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc
     2281 gtaaaggagt aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat
     2341 ggattaccac gcgctgaatg cgatgcttaa cctctacgat agcgcaggtc gcattcagtt
     2401 cgataaagac cgccaggccg ttgacgcctt tatggcgacg catgtgcgtc cgaacagtgt
     2461 gaccttcagt agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga
     2521 aagcgttctt aatcgctact ctcgcgactt tgtcattacg ctgtttgccc acgcacacac
     2581 cagcggtttt cgtttccata cattcctcgg ggcatggaag ttttacacca gctatacgtt
     2641 gaagacattc gacggtaagc gttatctgga agattttgcc gatcgagtca cgatggtggc
     2701 gctaacgctg gcacaaggcg atgaaacact ggcgttgcaa ctgaccgatg agatgctgtc
     2761 aggacgcttt cagcctgcaa caccgacatt cctcaactgt ggtaagcagc agcgcggcga
     2821 actggtttcc tgttttttgc tgcgtattga agacaatatg gagtcgattg gccgggcggt
     2881 aaattccgca ctgcaactgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct
     2941 gcgggaagcg ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt
     3001 gatgaaaatg ctggaagacg cattttccta tgccaaccaa ctcggcgctc gtcagggggc
     3061 tggtgcagtc tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg
     3121 ggaaaatgcc gacgaaaaaa tccgcattaa aacactgtcg cttggcgtgg tgatcccgga
     3181 tatcactttc catctggcca aagagaatgc gcagatggcg ctgttttcgc cctatgacgt
     3241 tgagcgagtt tatggcaagc cgtttgccga tatcgccatt agcgaacact atgacgaact
     3301 ggttgccgat gaacgcatcc gcaaaaaata cctcaacgcc cgtgatttct tccagcgact
     3361 ggcagaaatc cagtttgagt ccggctatcc ctacatcatg tatgaagaca cggtaaaccg
     3421 tgctaaccct atcgccgggc gcataaatat gagtaatcta tgctcagaaa ttttgcaggt
     3481 taacagcgcc tcagagtatg acgagaatct cgactatgcc cgtacaggcc atgatatttc
     3541 ctgcaattta ggttcgttga atattgccca caccatggat tcccccaact ttgcccgcac
     3601 ggtagagact gccgtacgcg gtttaacggc ggtgtcagat atgagtcata tccgcagcgt
     3661 gccgtccatc gaagccggaa atgccgcctc acacgccatc gggctggggc agatgaattt
     3721 acacggttat ctggcgcgag aaggcatcgc ttatggttcg ccggaagcac tggatttcac
     3781 caatctctat ttctatacca tcacctggca cgcactgcgt acctcgatgt tgctggcacg
     3841 cgaacgcggt gaaaccttcg ccgggttcaa acagtcacgc tatgccagta gtgaatattt
     3901 cagccaatat ctgcaaggga actggcagcc gaaaacgacg aaagttggcg aactgtttgc
     3961 ccgtagcggt attacgttac ctacccgtga gatgtgggcg caactgcgcg acgacgtgat
     4021 gcgctacggc atttataacc agaatcttca ggcggtgccg ccaaccggtt ctatctctta
     4081 tatcaaccat gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga
     4141 gggcaaaaca ggacgcgttt actaccctgc cccgtttatg actaacgaga atctggcgct
     4201 gtatcaggat gcttacgaaa tcggcgcaga aaagatcatc gacacctacg cggaagcgac
     4261 tcgccatgtc gatcagggtt tgtcgctgac gctgtttttc cccgataccg ccaccactcg
     4321 cgatatcaac aaagcgcaga tttacgcctg gcgcaaaggt atcaaaacgc tctattacat
     4381 tcgcctgcgt cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact
     4441 ttaaggaata tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga
     4501 cgataaagat ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt
     4561 gccgctgtcg aacgatattc ctgcctggca gacattaact gtcgtagaac aacaactgac
     4621 gatgcgcgtt tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc
     4681 ttcgctgatg cccgatgcac tcacgcctca tgaagaagcg gtattatcga atatcagctt
     4741 tatggaagcg gttcatgccc gctcttacag ttcgattttc tcgacgctgt gtcagaccaa
     4801 agatgtcgat gccgcctacg catggagtga agaaaacgca ccgttgcagc gaaaagctca
     4861 gattattcag caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt
     4921 tcttgaatct tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg
     4981 aaagctgacc aatacagcag atctgatccg tctgattatc cgcgatgaag cagtccacgg
     5041 ttactacata ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga
     5101 agagttgaag agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata
     5161 caccgatgag ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta
     5221 caacgccaat aaggctttga tgaatctgga ctacgaaccg ttatttcccg cagaaatggc
     5281 ggaagtgaat ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt
     5341 cttttccggt tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga
     5401 ctggaatttc tgagggtgtt attttcaaaa atatcactac ccgcagcagg gaaataattc
     5461 ccgccaaata gctttttatc acgcaaatat tctgtagtga tctacactga tactctgttg
     5521 cattattcgc ctgaaactac aatattcagg cgttttttcg ctatctatga caaaaaatat
     5581 caacttttct cgatttgctc tcagccctta tatcacggga aattccggcg atttgctcgc
     5641 atcaatattc atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt
     5701 agaaaaaagt gactatttcc attgggtaat atatcgacat aaacaaataa aggaatcttt
     5761 ctattgcatg gcaattaaat tagaaattaa aaatctttat aaaatatttg gcgagcatcc
     5821 acagcgagcg ttcaaatata tcgaacaagg actttcgaaa gaacaaattc tggaaaaaac
     5881 tgggctatcg cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt
     5941 catcatggga ttatccggct cgggtaaatc cacaatggta cgccttctca atcgcctgat
     6001 tgaacccacc cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgatgc
     6061 cgaactccgt gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat
     6121 gccgcatatg accgtgctgg acaatactgc gttcggtatg gaattggccg gaattaatgc
     6181 cgaagaacgc cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc
     6241 ccacagctac ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc
     6301 gttagcgatt aatccggata tattattaat ggacgaagcc ttctcggcgc tcgatccatt
     6361 aattcgcacc gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat
     6421 tgtctttatt tcccacgatc tcgatgaggc tatgcgtatt ggcgaccgaa ttgccattat
     6481 gcaaaatggt gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa
     6541 tgattatgtc cgtaccttct tccgtggcgt tgatattagt caggtattca gtgcgaaaga
     6601 tattgcccgc cggacaccga atggcttaat tcgtaaaacc cctggcttcg gcccacgttc
     6661 ggcactgaaa ttattgcagg atgaagatcg cgaatatggc tacgttatcg aacgcggtaa
     6721 taagtttgtc ggcgcagttt ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg
     6781 tcttgatgcg gcgctgattg atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga
     6841 gttgctctct catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca
     6901 gtatgtcggc atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa
     6961 taatggctga tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgtgcaat
     7021 ccgcagacgc ctggggtaca ccggcgactg caccgactga cggcggtggt gctgactggc
     7081 tgaccagtac gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa
     7141 cgctgatccc gctcgacagt tgggtcactg aagggatcga ctgggttgtt acccatttcc
     7201 gtcccgtctt ccagggcgtg cgcgttccgg ttgattacat tctcaacggt ttccagcaat
     7261 tgctgctggg tatgcccgca ccggtggcga ttatcgtttt cgctctcatc gcctggcaga
     7321 tttccggggt cggaatgggc gtggcgacgc tggtttcgct gattgccatc ggcgcaatcg
     7381 gtgcctggtc gcaggccatg gttaccctgg cgctggtgtt aaccgccctg ctgttctgta
     7441 tcgtcattgg tttgccgctg gggatctggc tggcgagaag tccgcgagcg gcgaaaatta
     7501 ttcgtccact gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtgccaatcg
     7561 tcatgctatt tggtatcggt aacgtgccgg gcgtggtggt gacgatcatc tttgctctgc
     7621 cgccgattat ccgtctgacc attctgggga ttaaccaggt tccggcggat ctgattgaag
     7681 cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg
     7741 cgatgccgac cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg
     7801 tcattgcctc gatgattgcc gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc
     7861 gtctggatat ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc
     7921 tcgaccgcct gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt
     7981 acaccactgg ccctgtcggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc
     8041 cggtaacgcc gggcatatca ccctgccaaa aaaaggaata acaatgcgac atagcgtact
     8101 ttttgcgaca gcgtttgcca cgcttatctc tacacaaact tttgctgccg acctgccggg
     8161 caaaggcatt acagttaatc cagttcaaag caccatcacc gaagaaacct tccagacact
     8221 gctggtcagt cgtgcgctgg agaaattagg ttataccgtc aataaaccca gcgaagtaga
     8281 ttacaacgtt ggctacacct cgcttgcttc cggcgatgca accttcaccg ccgtgaactg
     8341 gacgccgctg catgacaaca tgtacgaagc tgccggtggc gataagaaat tttatcgtga
     8401 aggggtattt gttaacggcg cggcacaggg ttacctgatc gataagaaaa ccgccgacca
     8461 gtataaaatc accaacatcg cacaactgaa agatccgaag atcgccaaac tgttcgatac
     8521 caacggcgac ggtaaagcgg atttaaccgg ttgtaaccct ggctggggct gcgaaggtgc
     8581 catcaaccac cagcttgccg cgtatgaact gaccaacacc gtgacgcata atcaggggaa
     8641 ctacgcagcg atgatggccg acaccatcag tcgctacaaa gagggcaaac cggtgtttta
     8701 ttacacctgg acgccgtact gggtgagtaa tgagctgaag ccagggaaag atgtggtctg
     8761 gttgcaggtg ccgttctccg cactgccggg cgataaaaat gccgatacca aactgccgaa
     8821 tggcgcgaat tacggcttcc cggtcagcac catgcatatc gttgccaaca aagcctggac
     8881 cgagaaaaat ccggcggcag ccaaactgtt tgccattatg cagttgccag tggccgatat
     8941 taacgcccag aacgccatta tgcatgacgg caaagcctca gaaggcgata ttcagggcca
     9001 cgttgatggc tggatcaaag cccaccagca gcagttcgat ggctgggtga atgaggcgct
     9061 ggcagcgcag aagtaatttt tattcaggcg gataaggcgt ttttgcctca tccgccgctc
     9121 tgtacacaat gcctgatgcg acgctgacgc gtcttatcat gcctacaaaa tacactcatt
     9181 cgtatggcgg ataaggcgtt ttcgccgcat ccgccgttct gtgcacaatg cctgatgcga
     9241 cgctggcgcg tcttatcatg cctacaaacc acatcgcaca tttcaacaat ctatctttca
     9301 tcccatattc atcaacatcc gctattattg atttccagct taatcatcac ctgatgaaca
     9361 aaaataatga ctaaacctaa tcatgagctt agcccggcgc tgatcgtgct gatgtctatc
     9421 gccaccggtc tggcggtagc cagtaactat tacgcccagc cattgctcga caccatcgcg
     9481 cgtaactttt ccctttccgc cagttcggca ggctttattg ttaccgccgc gcagttgggc
     9541 tacgccgcag gtctactgtt tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg
     9601 attgtctcga tgaccttact ggcagcgggc ggtatgttaa tcactgccag cagtcagtcg
     9661 ctggcgatga tgatcctcgg tacggcatta accggtttat tctctgtcgt ggcacaaatt
     9721 ctggttccgc tggcagcgac gctggcttca ccggacaaac gcggcaaagt ggttggcact
     9781 attatgagcg gtctgctgtt ggggatcttg ctggcacgga cagttgccgg attgctggcg
     9841 aatctcggcg gctggcgcac cgtcttttgg gttgcctcgg tgttaatggc actgatggcg
     9901 ctggcattat ggcgtggtct gccacaaatg aaatcagaaa cccacctcaa ctacccacag
     9961 ttgttaggtt ccgttttcag catgtttatc agcgataaga tcctgcgcac ccgcgcattg
    10021 ctgggctgcc tgacctttgc caacttcagc attctctgga cctcaatggc ctttttgctt
    10081 gccgctccac cttttaacta cagcgatggt gtcattggtc tgtttggact tgcgggagct
    10141 gccggagcgt tgggcgctcg tccggcgggc ggttttgccg ataagggtaa atcgcaccac
    10201 accacaactt tcggtctgct gctgctatta ctttcatggc tggcgatctg gtttggtcac
    10261 acttccgtac tggcgctgat tatcggcatc ctggtgctgg atctcaccgt acagggcgtg
    10321 catatcacta accagacggt aatttatcga atacaccctg atgcgcgtaa tcgcctgacc
    10381 gcaggttaca tgaccagcta ctttattggc ggtgccgccg gttcgctaat ttcagcctca
    10441 gcctggcaac atggcggttg ggctggcgtt tgtctggctg gcgcgacgat tgccctggtt
    10501 aacttactgg tctggtggcg aggttttcat cgtcaggaag ccgcaaatta agcaatcagc
    10561 aaccgttttc gtggctttac acttataagg gtgttaagaa gcccatcagt ctgataaggt
    10621 taagatattc attcagtcta tttataatat taacaatcgt taagcgtaca ctctatggaa
    10681 agccctactc cacagcctgc tcctggttcg gcgaccttca tggaaggatg caaagacagt
    10741 ttaccgattg ttattagtta tattccggtg gcctttgcgt tcggtctgaa tgcgacccgt
    10801 ctgggattct ctcctctcga aagcgttttt ttctcctgca tcatttatgc aggcgcgagc
    10861 cagttcgtca ttaccgcgat gctggcagcc gggagtagtt tgtgggttgc tgcactgacc
    10921 gtcatggcaa tggatgttcg ccatgtgttg tatggcccgt cactgcgtag ccgtattatt
    10981 cagcgtctgc aaaaatcgaa aaccgccctg tgggcatttg gcctgacgga tgaggttttt
    11041 gccgccgcaa ccgcaaaact ggtacgcaat aatcgccgct ggagcgagaa ctggatgatc
    11101 ggcattgcct tcagttcatg gtcatcgtgg gtatttggta cggtaatagg ggcattctcc
    11161 ggcagcggct tgctgcaagg ttatcccgcc gttgaagctg cattaggttt tatgcttccg
    11221 gcactcttta tgagtttcct gctcgcctct ttccagcgca aacaatctct ttgcgttacc
    11281 gcagcgttag ttggtgccct tgcaggcgta acgctatttt ctattcccgt cgccattctg
    11341 gcaggcattg tctgtggctg cctcactgcg ttaatccagg cattctggca aggagcgccc
    11401 gatgagctat gaggttctgc tgcttgggtt actggttggc gtggcgaatt attgcttccg
    11461 ctatttgccg ctgcgcctgc gtgtgggtaa tgcccgccca accaaacgtg gcgcggtagg
    11521 tattttgctc gacaccattg gcatcgcctc gatatgcgct ctgctggttg tctctaccgc
    11581 accagaagtg atgcacgata cacgccgttt cgtgcccacg ctggtcggct tcgcggtact
    11641 gggtgccagt ttctataaaa cacgcagcat tatcatccca acactgctta gtgcgctggc
    11701 ctatgggctc gcctggaaag tgatggcgat tatataaccc acaagaatca tttttctaaa
    11761 acaatacatt tactttattt gtcactgtcg ttactatatc ggctgaaatt aatgaggtca
    11821 tacccaaatg gatagttcgt ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg
    11881 ccacgaagat tttccttatc aggagatcct tctgactcgt ctttgcatgc acatgcaaag
    11941 caagctgctg gagaaccgca ataaaatgct gaaggctcag gggattaacg agacgttgtt
    12001 tatggcgttg attacgctgg agtctcagga aaaccacagt attcagcctt ctgaattaag
    12061 ttgtgctctt ggatcatccc gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg
    12121 cggttggatc gaacgtcgtg aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac
    12181 ggaaaaaggt cacgagtttt tgcgcgaggt tttaccaccg cagcataact gcctgcatca
    12241 actctggtcc gcgctcagca caacagaaaa agatcagctc gagcaaatca cccgcaaatt
    12301 gctctcccgt ctcgaccaga tggaacaaga cggtgtggtt ctcgaagcga tgagctaacg
    12361 cgtcatctcg ctcaaaaatc cagatttata taagaaaaaa tgaccggcca gcatcgcaac
    12421 atgctggcct ttttggcaag caggtcggct cagccgatga gttaagaaga tcgtggagaa
    12481 caatatgagc gcaaatgcgg agactcaaac cccgcagcaa ccggtaaaga agagcggcaa
    12541 acgtaagcgt ctgctcctcc ttctcacctt gctctttata attattgccg tagcgatagg
    12601 gatttattgg tttttggtac tgcgtcattt cgaagaaacc gatgacgcat acgtggcagg
    12661 gaatcagatt caaattatgt ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa
    12721 caccgatttt gtaaaagaag gcgatgttct ggtcactctc gacccgacag atgctcgcca
    12781 ggcgtttgaa aaagccaaaa ctgcactggc ttccagcgtt cgccaaaccc accagctgat
    12841 gattaacagc aaacagttgc aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc
    12901 acaaagcgac tacaaccgcc gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga
    12961 gctgcaacac gcccgcgacg ccgtcaccag tgcccaggcg caactggacg tcgctattca
    13021 acaatacaat gccaatcagg cgatgattct ggggactaaa ctggaagatc agcctgccgt
    13081 gcaacaggct gccaccgaag tacgtaacgc ctggctggcg ctagagcgta ctcgtattgt
    13141 cagtccgatg accggttatg tctcccgccg cgcggtacag cctggggcac aaattagccc
    13201 aacgacgccg ctgatggcgg tcgttccagc caccaatatg tgggtggatg ccaactttaa
    13261 agagacgcag attgccaata tgcgtatcgg tcagccggtc actatcacca ccgatattta
    13321 cggcgatgat gtgaaataca ccggtaaagt agttggtctg gatatgggca caggtagcgc
    13381 gttctcactg cttccggcgc aaaatgcgac cggtaactgg atcaaagtcg ttcagcgtct
    13441 gcctgtgcgt atcgaactgg accagaaaca gctggagcaa tacccgctgc gcattggttt
    13501 gtccacgctg gtgagcgtca ataccactaa ccgtgacggt caggtactgg caaataaagt
    13561 acgttcaaca ccggtcgcgg taagtaccgc gcgtgaaatc agcctggcac ctgtcaataa
    13621 actgatcgac gatatcgtaa aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac
    13681 agcaaaaacc gctggaaggc gcgcaactgg tcattatgac gattgcgctg tcactggcga
    13741 cattcatgca ggtgctggac tccaccattg ctaacgtggc gatccccact atcgccggga
    13801 atctgggctc atcgctcagc cagggaacgt gggtaatcac ttctttcggg gtggcgaatg
    13861 ccatctcgat cccgcttacc ggctggctgg caaagcgcgt cggggaagtg aaactgttcc
    13921 tttggtccac catcgccttt gctattgcgt cgtgggcgtg tggtgtctcc agcagcctga
    13981 atatgctgat cttcttccgc gtgattcagg ggattgtcgc cgggccgttg atcccgcttt
    14041 cgcaaagtct attgctgaat aactacccgc cagccaaacg ctcgatcgcg ctggcgttgt
    14101 ggtcgatgac ggtgattgtc gcgccaattt gcggcccgat cctcggcggt tatatcagcg
    14161 ataattacca ctggggctgg atattcttca tcaacgtgcc gattggcgtg gcggtggtgt
    14221 tgatgacact gcaaactctg cgcggacgtg aaacccgcac cgaacggcgg cggattgatg
    14281 ccgtggggct ggcactgctg gttattggta tcggcagcct gcagattatg ctcgaccgcg
    14341 gtaaagagct ggactggttt tcatcacagg aaattatcat tcttaccgtg gtggcggtgg
    14401 tggctatctg cttcctgatt gtctgggagc tgaccgacga taacccgata gtcgatctgt
    14461 cgttgtttaa gtcgcgcaac ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc
    14521 tctacttcgg cgctattgtt ctgctgccgc agttgttgca ggaggtctac ggttacacgg
    14581 cgacctgggc aggtttggcc tctgcgccgg tagggattat tccggtgatc ctgtcgccga
    14641 ttatcggccg cttcgcgcat aaactggata tgcggcggct ggtaaccttc agctttatta
    14701 tgtatgccgt ctgcttctac tggcgtgcct atacctttga accaggtatg gattttggcg
    14761 cgtcggcctg gccgcagttt atccaggggt ttgcggtggc ctgcttcttt atgccgctga
    14821 ccaccattac gctgtctggt ttgccaccgg aacgactggc ggcggcatcg agcctctcta
    14881 actttacgcg aacgctggcg gggtctatcg gcacgtcgat aaccacgacc atgtggacca
    14941 accgcgagtc gatgcaccat gcgcagttga ctgagtcggt aaacccgttc aacccgaatg
    15001 cccaggcgat gtacagtcaa ctggaagggc ttgggatgac gcaacagcag gcatcaggct
    15061 ggattgccca gcagatcacc aatcaggggc tgattatttc cgccaatgag atcttctgga
    15121 tgtcagccgg aatattcctc gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg
    15181 gcgcaggtgg cggcggaggc ggtgcgcact aagtacaact aagccagttc atttgaactg
    15241 gcttttttca attaattgtg aagatagttt actgactaga tgtgcagttc ctgcaacttc
    15301 tctttcggca gtgccagttc ttcgttgctg ttgatgcgta cgtcacgttc cagaatgcta
    15361 cgcgcaatat cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca ctggtagacg
    15421 ttcagttccg ggatctgatt ctgatcctgc actttcagca cgtcttccat tgccgctttc
    15481 caggcatcag caacacgctg ctcatctggc gtaccaatca gactcatata aaaaccggtg
    15541 cggcagccca ttggcgagat atcgataatc tctacaccct taccgttaag atggttacgc
    15601 ataaaaccag caaacaggtg ctccagggta tggatccctc tttctggcat cacttctttg
    15661 ttcggcacgc agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt
    15721 gttttcgcca cccgaactgc aggcgcttcc atccgggtat gatcgactgt gaagctatct
    15781 aacaacggca tttagccacc tccggtaatt tttttaaaaa ttttctgaac tctttcttcc
    15841 caggcgagtc tgagtatatg aaagacgcgc atttgttatc atcatccctg aattcagaga
    15901 tgaaattttg gccactcacg agtggccttt ttcttttctg tcaggcgtgt ttttccagcc
    15961 acaccgcaaa cggttcggta tcagcggctt ccatttcctg ctgacggcgt tcagacgcct
    16021 cgcgctcggc tacaaaatcc tcttcgcgca gaatttccag cggctcttca cgcagcagat
    16081 tacggtaagc ttctgcaaat gctttgcctg ttccgccaat accagtatca atcatagacc
    16141 ttaagatacg ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc agttcatcac
    16201 acactttctg atacgcttcg ccgccgttaa tactatccag cgtttgcgcg acgcgtttca
    16261 gatcgcggaa cagatcttta cccacctgcg gtaacgggaa ctgtgcggtt tcgcagccga
    16321 tacccagcgt cagacccggt ttgcgacctt cgaggatcac ccggttccag ttaacgcgtg
    16381 tacaggcaag ttcgctactg ctcatttccg gtgcatcagc cagcgcacac cagaccataa
    16441 acaggtcgag gaatcgcacc tgctgttcat ctacaccaat cggcgagaac gggttgatgt
    16501 ccagcgaacg cacttcaata tattcaatgc cgccacgtaa cagcgcatca gaaggcgact
    16561 cgccgctgcg ggtaacgcgt tttggacgaa tcggcgcgta cagttcgttt tcaatctgca
    16621 acacgttgct gttgatttgc agcctcttac cgtctttctc aataccaatc ttcgcgtact
    16681 cttccgatgg cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa agatcgttga
    16741 aggtaatacc aagattgctt tgcgatttat tggtatagcc gagatcgctc aaacgaagag
    16801 aggtcgcata cggcaggtaa tacataccgc actcggtttt ctcaaacggc agcgacgttg
    16861 gttttccttg caggaaagaa gaacaaatcg ccggagatgc accaaacaga taaggaatga
    16921 cccaaccgaa acgatagtaa ttgcggataa cgcggaaata gcccgcagaa attttctctt
    16981 tggcatcagc gcccgagata tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga
    17041 aattgtagtg cacgccggaa atggtttgca tcagcgcgcc gtagcgattt ttcagccctt
    17101 cacgatacag cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt
    17161 cctgaccttc tgcgatgtag catggcatac ttaacggcca catccgctca tcgcccatat
    17221 tgcgcgccgt ataacgatgc agatcgcgca taaaggtcaa catatgttca atatcaccat
    17281 ccactggtgt aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg
    17341 tcagtgcgga acctaatgct tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa
    17401 cacgcaaagt ttcgcgctcc agcccacgct gtatcccctt taacgcctga ggatgttttt
    17461 ccagccaggc cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat
    17521 cgttttaatt agcataactg taatggtgac catatgtgca ggcctacaat tagtgccacc
    17581 acatcatgcc ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc
    17641 acaaaaaaaa gattactggc ccccaggaga tgcgcatcca tcccgctaac agacacagta
    17701 aatcgccaac cacgggcatc cagcttaata atagcgtgac tgcaccatag cgtttcagcc
    17761 agccggtagc tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga
    17821 taacgttagt taaccctcca aggctattac ccattgttgc tgttaaaact aaaacccagg
    17881 gataactgat cccggaaagc aacattgcca ccagcacgac ttcggagttg ccgggtaata
    17941 gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag cgataacgct tcactcacag
    18001 caagcgaaca tccacggcgt ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc
    18061 atcttcaaag accacacact gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa
    18121 tgtgtctggc gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg cgtcaaaata
    18181 acggcgtaat cccaggtgcg ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc
    18241 tacagccatt gggcgacgac catgccaact tttcaccact tcaacaagag gaagcggctc
    18301 gacactatcc agcagcatac ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg
    18361 gtcgagatcg gcctgattca gctcaataat tgcctgagca atacgccagg tgggcgatcc
    18421 attaagcgca atcattgcct gaacatcgta ctgaagaccg tagtgcccta atacttcgcg
    18481 ccacgcttta cggtgcgtag gctccgtatc caggattgtg ccatccatat caaaaattaa
    18541 acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc
    18601 gtaattgctt gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag
    18661 tagttattta gaaatgagat atttttgaag gaaatttttg ggagaagatg gtgcatccgg
    18721 gaggattact cactggcgtt cgcccttcgg gccgttgctg acgcaacgtt atcctccctg
    18781 gtgcctgcgg ttaataccgc tatctcttcg ataccttcat tgctgaaggt t
//
LOCUS       DAPHDX010000083        18365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPHDX010000083 DAPHDX010000000
VERSION     DAPHDX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.083"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..261
                     /locus_tag="RH058_002936"
     CDS             <1..261
                     /locus_tag="RH058_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV3138496.1"
                     /translation="VLYFHYFMMTIKSTLEKFAPSTAQKNINIDILSQLFFPCPPLEE
                     FHRIVDKVQNLLSVCDVLRAYIQSAQQTQLHLADALTDAAIN"
     gene            482..823
                     /gene="symE"
                     /locus_tag="RH058_002937"
     CDS             482..823
                     /gene="symE"
                     /locus_tag="RH058_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDV3138497.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDCSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPVAEESELMQSLRQVCKLSARKQKQVQEF
                     IGVIAGKQKVA"
     gene            complement(870..2033)
                     /locus_tag="RH058_002938"
     CDS             complement(870..2033)
                     /locus_tag="RH058_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HDV3138498.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GLHSRLLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(2081..2962)
                     /locus_tag="RH058_002939"
                     /pseudo
     CDS             complement(2081..2962)
                     /locus_tag="RH058_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(3059..3223)
                     /locus_tag="RH058_002940"
     CDS             complement(3059..3223)
                     /locus_tag="RH058_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV3138499.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAMRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3400..4812
                     /locus_tag="RH058_002941"
     CDS             3400..4812
                     /locus_tag="RH058_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV3138500.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSMALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWVELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(5054..6010)
                     /locus_tag="RH058_002942"
     CDS             complement(5054..6010)
                     /locus_tag="RH058_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV3138501.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTEFANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            6477..7709
                     /gene="mdtM"
                     /locus_tag="RH058_002943"
     CDS             6477..7709
                     /gene="mdtM"
                     /locus_tag="RH058_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV3138502.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            7750..9030
                     /locus_tag="RH058_002944"
     CDS             7750..9030
                     /locus_tag="RH058_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV3138503.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIILRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRSHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            9146..10297
                     /locus_tag="RH058_002945"
     CDS             9146..10297
                     /locus_tag="RH058_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV3138504.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDTSRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10307..11074
                     /locus_tag="RH058_002946"
     CDS             10307..11074
                     /locus_tag="RH058_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138505.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            11071..11328
                     /locus_tag="RH058_002947"
     CDS             11071..11328
                     /locus_tag="RH058_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV3138506.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            11393..12253
                     /locus_tag="RH058_002948"
     CDS             11393..12253
                     /locus_tag="RH058_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV3138507.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            12321..13499
                     /locus_tag="RH058_002949"
     CDS             12321..13499
                     /locus_tag="RH058_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138508.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13512..14066)
                     /gene="kptA"
                     /locus_tag="RH058_002950"
     CDS             complement(13512..14066)
                     /gene="kptA"
                     /locus_tag="RH058_002950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV3138509.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            14316..14999
                     /locus_tag="RH058_002951"
     CDS             14316..14999
                     /locus_tag="RH058_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138510.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14996..15457
                     /locus_tag="RH058_002952"
     CDS             14996..15457
                     /locus_tag="RH058_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV3138511.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15470..16642
                     /gene="iadA"
                     /locus_tag="RH058_002953"
     CDS             15470..16642
                     /gene="iadA"
                     /locus_tag="RH058_002953"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV3138512.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            16707..17618
                     /gene="hypT"
                     /locus_tag="RH058_002954"
                     /gene_synonym="qseD"
     CDS             16707..17618
                     /gene="hypT"
                     /locus_tag="RH058_002954"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV3138513.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(17611..18003)
                     /gene="iraD"
                     /locus_tag="RH058_002955"
     CDS             complement(17611..18003)
                     /gene="iraD"
                     /locus_tag="RH058_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV3138514.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHILAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(18000..18083)
                     /gene="idlP"
                     /locus_tag="RH058_002956"
     CDS             complement(18000..18083)
                     /gene="idlP"
                     /locus_tag="RH058_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV3138515.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         4325 a         4654 c         4542 g         4844 t
ORIGIN      
        1 gttctttatt ttcattattt catgatgaca ataaaatcca ctctggaaaa attcgcccca
       61 tcaactgcgc aaaaaaacat caacattgat atattaagtc aattgttttt cccatgtcca
      121 ccattagaag aattccatcg tattgttgat aaagttcaaa acttattatc agtttgcgat
      181 gttctaagag cctacattca atccgcccag caaacccaac tgcaccttgc agacgcactc
      241 actgacgcgg ctataaacta attttctcgc cctcataaaa ccaataaagc ggctcaatag
      301 ccgctttatt cacatcacca aaaattatat ttcacgctaa ttttcatctt attttcatta
      361 atttttagct ggactttccc tgtatttact gatgatatat ccaggtattt agcgcggtgg
      421 ggatgtgcgc caacacaccc gcaccgctca tcacaatcac tattttcaag gaatagcagt
      481 tatgactgac acgcattcta ttgcacaacc gttcgaagca gaagtctccc cggcaaataa
      541 ccgtcaatta accgtgagtt atgcgagccg ctacccggat tgcagccgta ttcccgccat
      601 caccctgaaa ggtcagtggc tggaagccgc cggttttgcc accggcactg tggtagatgt
      661 caaagtgatg gaaggctgta ttgtcctcac cgcccaaccc cccgtcgccg aagagagcga
      721 actgatgcag tcactgcgcc aggtgtgcaa gctgtcggca cgtaaacaga agcaggtgca
      781 ggaatttatt ggggtgattg ccggtaaaca gaaagttgcc tgatacattg acccggtagc
      841 accagtgcta ccgggtatta ttcgccgcgt tataccaagg cgctctccat ttgtttttca
      901 aatgcctgac gaattaatcc cataatgtaa ggaagttgtg cgaggtcgct gaaaccaatt
      961 tccacatcac cattacccca gcgcccaaca tttgtcacat ctttggcaat acctttcgga
     1021 tcgaccagtt catgaaactg catattaagc gacaatcgca gtcggctttt ttgcggcaca
     1081 acatcaacaa aattcgtttc agctttaaac gcaatataga gcttcaatac ttcctgcgtg
     1141 atccctgcgt ccaggcgcat aatttcatcg cgaagatgat caaataacaa ccggctatgc
     1201 aagccatcag caagaaaagg ataatcactc aggctgtaac tggttttgtt ctccggctgt
     1261 ccccgataaa cagctaaaac ctcttcagga agagaaggcg cttgccagac ctgaaccgca
     1321 agctccgcca gacgatcggc tcgggcgtga atggcagctt catcccattt ttcgcactgt
     1381 ccaagaccga tattcaaata gagcgggcta tgcttaaagc cgtcttcaat atcgcgtttt
     1441 tctgcgaaag gtctgtcact gtaacgggag ttatagcgcg ttaaagtgag attccccaac
     1501 gtatgcaaca gttctttatg aatacgctgc cagtcgcttc ccagctcttc gcgccattta
     1561 gccgacaaat tttcgttctg cggcataata tgttcaatcg taaactcttc cacgcgctct
     1621 ctgcgtttat cgttttccag tcgtcgtaac cagtagctgc gactacggaa gttatagaga
     1681 tcgcgaactt ttaattcccg tttaaattca tcatcgttgg ggaaacgacg atatgaaggt
     1741 agattcaaaa aatgtacctg aatactttcc agatattttt ctttattaat gaccttataa
     1801 aaagtagcaa acgtcttgtt aagagaattc gtcggaatcg cacatactgc acggcggaaa
     1861 acataagatt caattaaacg aattatgctc aggaaatcct cgtgagacaa aacgccattt
     1921 ttatagtcat gataaagcgc cagtaagaaa ggatacgcca catcgacctt taactcgcgc
     1981 aaatcctgaa aagccgtagc aagcgatttg tcactttctt ttcccaatgc cattgcacag
     2041 taatactccg cgtagatgtg aatatcttca accagtcgat ctacgccttt ttcagcaaca
     2101 ctctggctgc gggcatggag tttaaatgcc tcatagactt catctgtccg agggatctcc
     2161 cccgttttta ccgtcagata atgacgcata aaactgtcaa aatattcgct gtaaccttgc
     2221 tgaccaaagg cgacttccat tggacgccag tgatcttcat acaaccgggt ttgatgctct
     2281 ggttcgaggc ccatcagaat aaaattgcgc accagatcgg cctgacttaa cgccttaccg
     2341 gtggagttca tactttcaaa aatcagttgc ggattatcct gaccacgatt aagcgccaca
     2401 tcgacaatta ataactttgc taacccacga cataagggga tcaagtcatc accgagtttg
     2461 gcaatttgtt catcaaagaa agtaaagttt ccattattcg atgggaatag ttttctggca
     2521 ttggtctttg ttttattaac gccagcaaac tgtctttatc agtctcggtc agcagtaatt
     2581 taaaaccttt ctcgccggac tcataaggat tgagcaaata ataattacgc aatttcattg
     2641 ccgaaaagcc atcaaaaact tcgtcttcgc caagatggcg cgataaagcc tcaatcagca
     2701 acattgccgt cgtcagccgt tgttgaccat caatgaccag caacggagaa ataccagaaa
     2761 cctgatacaa cccctgctct atataaacaa ccgaaccgat aaaatgcgct gatatatcgt
     2821 cacgctttcc tgcacgaatg atgtcgtccc aaagttgccg acattgttgt tctgtccatg
     2881 aataagtccg ttgataaatg ggaataacaa actgctgcga acgttttaaa aaatcgagta
     2941 ggcgagcttc tgtagctttc attctgacgt ctttccgtga aacgatgatt tcgcaataat
     3001 aaccacttcc ttgtggaggt aatagagaaa tcctgtttat ccgcgacgaa catacccctc
     3061 actccacatc ctccctgcgt aacccgatat ccctcaaccg ctcatcactc atctgctgta
     3121 acactcgtcg ggtctgcatt tgccgccgcc aacgcctcat cgcctgccag agttgcacaa
     3181 gtctgataaa cggcgttttg gctttgtttt cgtgaaattc catctgctgc ctcctcatct
     3241 ttcagaggct tcatcatccg ccttctggca gttcacaata cagatgcaaa tattagtttt
     3301 atttaacata cagacaggtt aaaacagcat ctgcatccgg ttttttagct tcatctgtac
     3361 cggtttttct ctctgtatgg tctaaataag gaatacagca tgacgcgtta tcaacatctg
     3421 gcaactctgc ttgccgagcg gattgagcaa gggctgtatc gtcacgggga gaaattgccg
     3481 tcggtgcgca gcttaagtca ggagcacggc gtcagcatca gcaccgtgca gcaggcgtac
     3541 cagacgctgg agacgatgaa gctcatcact ccgcagccgc gttcgggtta ttttgtcgca
     3601 caacggaaag cccaaccgcc agtgccacca atgacgcgtc cggtgcagcg cccggtggaa
     3661 attacccagt gggatcaagt gctggatatg ctagaggcgc atagcgacag ttccattgtt
     3721 ccgttaagca aaagcacgcc ggatgtcgaa acgcccagcc tgaaaccact ctggcgggag
     3781 ctaagccggg tggtacagca taatctgcaa accgttctcg gttatgactt gttagccggt
     3841 cagcgagtgt tgcgagagca aattgcccgc ctgatgctcg acagcggctc ggtggtcacc
     3901 gccgatgaca tcatcatcac cagcggctgc cataactcga tgtcgatggc gttaatggcg
     3961 gtgtgtaaac caggcgatat tgtcgcggtc gaatctccct gttattacgg ttcgatgcag
     4021 atgctgcgag gcatgggcgt gaaagtgatt gaaatcccaa ccgatccaga aactggcatc
     4081 agcgttgaag cactggaact ggcgttggaa cagtggccga ttaaaggcat cattctggtg
     4141 ccaaactgta ataatccgct gggatttatt atgccggacg cacgcaaacg ggccgttctc
     4201 tctctcgctc agcgtcatga tattgtgatt tttgaagatg atgtctacgg cgaactggca
     4261 acggagtatc cgcgcccgcg gaccatccat tcatgggata tcgacgggcg agtgttgttg
     4321 tgcagctcgt tcagtaaaag tattgctcca ggcctgcgcg tgggttgggt cgcaccgggg
     4381 cgctatcacg ataaactgct gcatatgaaa tacgccatca gcagctttaa tgtaccgtcc
     4441 acgcaaatgg cggcggcaac gtttgtgctg gaaggtcact atcatcgcca tatccggcgg
     4501 atgcggcaga cttatcagcg caatctggcg ctttatacct gctggatacg ggaatatttt
     4561 ccctgcgaaa tctgtattac gcgcccgaaa ggcggatttt tactgtgggt agaattgcct
     4621 gaacaggtcg atatggtctg cgtcgcgcgg cagctgtacc gcatgaaaat ccaggtggcg
     4681 gcaggctcga ttttctcagc gtccggcaaa taccgtaatt gtctgcgcat caactgcgct
     4741 ttaccgctca gcgaaaccta tcgcgaagca ctaaagcaaa ttggcgaggc cgtgtatcag
     4801 gcaatggaat gaattcgaaa tagtggcgaa gcacctgatt tactttcacg aatcgcctgt
     4861 aactcgtttt atctttatag cgacatcgcc acgttccggc atagcttagg caaagtgtgc
     4921 tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaacca gcaaattcaa
     4981 tagattgcag aggtaatgta ggtctgataa gcgtagcgca tcaggctgct ttgcgtttgt
     5041 catcagcctc tgattagtac tcgatccatg gttcgcgctt gctttcaggc aagggctgat
     5101 ctaatgcctg catttgctct gccgaaagtc cggtatatcg ttgtatccat tcaggatcgg
     5161 ccccattctg caacaacaat cggaggaggc tccgttcccc ttctactttc ccttctttcc
     5221 tccccctctc ccttcccaac agccatccat cattatgaat tcgctctgca attgtcatta
     5281 ttcgctccct gtggtgtggc attcgcctgg taagttcgct gataaacgcc ttaaaacgcg
     5341 cttcatcgcc agtcagtaaa atgtaattta acagcgcagt tatctggctg tcattagcaa
     5401 actctgtaac cagtaatact accagttggt cgataagccc catcagatcg cgctggcgaa
     5461 tatgcttttg gatcaactcc aacagggcga ctctgcgatg ctgcacaatc tcgtcgtctg
     5521 gcacgacagt gacatccacc agcgggaacg ctgcgctata aagtttccgt gcggtagtcg
     5581 ggtcggcaaa ttcgtccagc cagcacaggg accagggata aggactacgg ctaccgtgat
     5641 aaaatagcat cgggatgacc agcggtagcg gccgacgttt atcatgctcg atatggcgct
     5701 gcatcaccgc catggaatag cgcatcaggc gaaaggccat atgaatgtcc tcgcggctct
     5761 gatgttcaat caccacatag atataaccat cgccttcgcg ggtctttacc gaccacagaa
     5821 tatcggaatg tagcgcccgc aatttttcat cgacaaagct ggcggattcc agttttaagc
     5881 tgtcgagatc gcacagttca cgtaaatctt taggtaagtg gatctccata aaatcccgcg
     5941 cggtgtcagg gtgcgtgaga aaggacttaa ataatgcgtc atgcggcgtg ctggttgtga
     6001 agtttgtcat ggcgatccgt cacctgctaa taagatggcc cgactgtagc gactaacaaa
     6061 accattctca cccggcaatt tctcgttccc cgaggcactt tccaggctat ttttgttcgt
     6121 ctgcaaacga aacatttttc cggaaacagg ctcgcgtttt acatttcctg taccaggcag
     6181 atattcccct ttccaccgcg ctcttttgat taaccgcaaa ttttaattac acttaaggtg
     6241 tatattttct atgcaaccca tcaattcaag aggtgtaatg tgctgatgac tatttgtaat
     6301 cgttatacat tctgacctgg agtcagaagg tatttctctg tctgtgtgtt cacaggcagt
     6361 gtggttgatt acatgaaatt cagtacattt gtccttctcg cctgcctttc gtcattaccg
     6421 acggtattga atttcgtttt ccccgttggg gttctccgga caaggagttg tttgttatgc
     6481 cacgtttttt tgcccgccat gccgccacgc tgtttttccc gatggcgttg attttgtatg
     6541 actttgccgc gtacctgtcg acggatctga tccagccggg gatcattaat gtggtccgtg
     6601 attttaatgc cgatgtcagt ctggccccgg cagccgtcag tctctatctc gcgggcggta
     6661 tggcgttaca gtggctgctg gggccgcttt ccgacaggat tggccgcagg ccggtgctga
     6721 ttaccggggc gctaatattt acccttgcct gcgctgcgac aatgttcaca acgtctatga
     6781 cacagtttct tatcgcgcgt gcaattcagg gcaccagtat ctgttttatt gccaccgttg
     6841 gttatgtcac ggtgcaggag gcgttcggcc agacaaaagg gatcaagttg atggcgatta
     6901 tcacctccat cgtactgatt gcgccgatta ttggcccgct ttccggcgca gctctgatgc
     6961 actttgtgca ctggaaagtc ctttttgcca tcattgcagt tatgggtttt atctcatttg
     7021 ttggcttact gctggcgatg ccagagacgg tgaagcgcgg cgcggttcca tttagcgcca
     7081 aaagcgtctt tcgcgatttt cgtaatgtct tttgcaatcg gctgttcctc tttggcgcag
     7141 caaccatctc tttaagctat atcccaatga tgagttgggt ggctgtctcg ccggtgatcc
     7201 tgatcgacgc gggcggctta acaacttcgc agttcgcctg gacacaggtt ccggtgttcg
     7261 gcgcggtgat tgtcgcgaat gccatcgtgg cgcgtttcgt taaagatccg accgaaccgc
     7321 gctttatctg gcgtgctgta cccattcaac tggtcggcct cgcactgttg attatcggca
     7381 atctgctgtc accgcacgtc tggctgtggt cggtgctggg caccagtttg tatgctttcg
     7441 ggattggttt gatcttcccg accttattcc gctttacgct gttttccaat aacttaccga
     7501 aagggaccgt ctccgcatcg ctgaatatgg tgatcctgat ggtgatgtct gtctcggtcg
     7561 aaatcggccg ctggctgtgg tttaacggcg gtcgcttgcc gtttcatctg ttagccgtcg
     7621 tggcgggcgt tatcgtcgtt ttcaccctgg caggattgct taatcgcgtg cgccaacatc
     7681 aggctgccga gctggcggag gagcggtgat ttttgcgcga tcctgccgtc aggctctatt
     7741 cttaacgtta tgaataaact cattgaactc agacgcgcca aaatgttggc gctctcttta
     7801 ctgcttatcg ccgctgctac ctttgtcgtt acgctgtttt tgccgcccaa tttttgggtg
     7861 agcggcgtga aggcgattgc tgaagcggcg atggtcggcg cgctggcgga ctggtttgcg
     7921 gtggtggcgc tgtttcgccg cgtgccgatt ccgatcattt ctcgtcatac ggcgattatc
     7981 ctgcgtaata aagaccggat tggcgaaaat ctcggtcagt tcgtgcagga aaaatttctc
     8041 gatacccagt cgctggtggc attgattcga cgtcacgaac cggcgttgtt gattggcaac
     8101 tggtttagtc agccggagaa cgcccgccgc gttggtcagc atctgttgca gatcatgagc
     8161 ggttttcttg aactgaccga tgatgcgcgt attcagcgcc tgcttaagcg cgcggtccat
     8221 cgggcgattg ataaggtcga tctttccgga accagtgcgt tgatgctgga gagtatgacc
     8281 aaaaacgatc gtcatcaggt gctgctcgat acgctgatcg cacagttgat tgctcttctc
     8341 cagcgcgata aatcgcgcaa gtttattgcc cagcaaattg ttcgctggct ggagagcgag
     8401 catccactga aagccaaaat tttgcctact gaatggctgg gcgaacatag cgcggagttg
     8461 gtttctgacg cggtgaattc tttgcttgat gatatcagcc gtgatcgttc gcatcagatc
     8521 cgccatgcgt ttgatcgcgc cacttttgcc ctgatcgaca agttgaaaaa cgatcctgaa
     8581 atggcagcgc gagccgatgc cgtaaaaagc tatctgaaag aagatgaagc ttttaaccgc
     8641 tatctcagtg aattgtgggg ggatttacgg gaatggctga aagcggatat caacagtgaa
     8701 gattctcgtg tgaaagaacg tatcgcgcgg gcgggtcaat ggtttggcga aacgttaatt
     8761 gccgatgatg ccttgcgggc gtcgttaaat ggtcatctgg aacaagccgc gcaccgcgtc
     8821 gcgcctgagt tttccgcatt cctgacgcgc catatcagcg atacggtaaa aagctgggat
     8881 gcgcgggata tgtcgcggca aatagagtta aatatcggca aagatctgca gtttatccgt
     8941 gtcaacggta cgctggttgg cggttgtatt gggctaattt tatatttgtt gtcgcagctc
     9001 ccggccttgt tccccctcgg caatttttag aaatccatga aaaactaagt agctaaccgc
     9061 gagagagatc aaataaacca cactaaaaga atggtaataa tgtggcctct tttcattatc
     9121 tcccgtggta cggggaagga aaatcatgtc acttgtcacc gatctacccg ccattttcga
     9181 tcagttctct gaagctcgcc agaaaggctt tctcaccgtc atggatctca aggagcgcgg
     9241 cattccgctg gttggcactt actgcacctt tatgccgcaa gagatcccga tggcagccgg
     9301 tgcggttgtg gtttcgctct gttccacctc tgatgaaacc attgaagaag cggagaaaga
     9361 tctgccgcgc aacctctgcc cgctgattaa aagcagctac ggcttcggca aaaccgataa
     9421 atgcccctac ttctactttt ctgatctggt ggtcggtgaa accacctgcg acggcaaaaa
     9481 gaaaatgtat gaatacatgg cggagtttaa gcccgttcat gtgatgcagc tgccgaacag
     9541 cgttaaggac gatacctcgc gtgcgttatg gaaagccgag atgctgcgct tgcaaaaaac
     9601 ggtagaagaa cgttttgggc acgagattag cgaagatgct ctgcgcgatg ccattgctct
     9661 gaaaaaccgc gaacgtcgcg cactggctaa tttttatcat cttgggcagt taaatcctcc
     9721 ggcgcttagc ggcagcgaca ttctgaaagt ggtttacggc gcaacattcc ggttcgataa
     9781 agaggcgttg atcaatgaac tggacgcgat gacagcccgc gttcgtcagc agtgggaaga
     9841 aggccagcga ctggacccgc gtccacgcat tttaatcacc ggctgcccga ttggcggcgc
     9901 agcagaaaaa gtggtgcgcg cgattgaaga gaatggcggc tgggttgtcg gttatgaaaa
     9961 ctgcaccggg gcgaaagcga ccgagcaatg cgtggcagaa acgggcgatg tctacgacgc
    10021 actggcggat aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct
    10081 gcaaatgctc agccagatgg tggaagaata tcaggtcgat ggcgtagttg atgtgatttt
    10141 gcaggcgtgc catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgtcagca
    10201 gcacaacatt ccttatatcg ctattgaaac agactactcc acctcagatg tcgggcagct
    10261 cagtacccgt gtcgcggcct ttattgagat gctgtaagga gtggcagtga catattcgat
    10321 tggcattgat tctggttcta ccgccaccaa agggatctta ctggcagacg gcgtgattac
    10381 gcgccgtttc ctcgttccaa caccctttcg tccggcaaca gcaattactg aagcctggga
    10441 aactctgcgc gaagggttag agacaacgcc gtttctgacg ctcaccggct atggacggca
    10501 actggtggat tttgccgata aacaagtgac ggaaatctcc tgtcatgggc tgggcgcacg
    10561 gtttcttgcg ccagcaacgc gcgcggtaat cgacatcggt ggtcaggaca gcaaagtgat
    10621 tcagcttgat gatgacggta acctgtgcga tttcctgatg aatgacaaat gcgcggcggg
    10681 caccgggcgt ttcctggagg tgatctcgcg cacgcttggc accagcgtcg agcaactcga
    10741 cagcattacc gaaaatgtca cgccgcacgc catcacgagt atgtgcacag tgtttgccga
    10801 atcagaagtg atcagcctgc gctcagcggg cgtcgcgcca gaagcgattc tcgccggagt
    10861 gattaacgcg atggcgcgga ggagtgccaa tttcattgct cgtctctcct gtgaagcgcc
    10921 gattctgttt actggtggcg ttagtcattg ccagacgttt gcccggatgc tggaatctca
    10981 tctgggaatg ccggtaaata cccatcctga tgcgcaattt gctggcgcaa ttggcgcggc
    11041 ggtaattggt caacgagtga ggacacgccg atgaaagagt ttttattttt atttcactcc
    11101 acggtcggcg tcatacaaac ccgcaaggcg ttgcaggcag cgggcatgac ttttcgcgtc
    11161 agcgatattc cccgtgattt acgcggcggc tgcgggttat gcatttggct gacctgtccc
    11221 cccggcgagg aaatacaatg ggtgatccct gggctgactg agtcaattta ttgtcagcag
    11281 gatggtgtct ggcgctgcat tgcacattac cgggtttctc cgcgttaagg attcattaag
    11341 gtcgctacgt atacttctcg atatcttatt gatttgcctt aaagggatta ccgtgacaaa
    11401 aagtatttcg ttaagtaaac gcatctccgt tattgtgatt ctttttgcca ttgtggcagt
    11461 gtgtactttc ttcgtgcaat cttgcgcccg taaaagcaat catgcagcat catttcagaa
    11521 ttaccacgcc accattgacg gcaaagaaat tgcaggcata acgaataata tctcttcgct
    11581 gacctggtcg gcacaaagca atactctttt tagcactatc aacaaacctg ccgccattgt
    11641 tgaaatgacc accaatggtg attttatccg cacaattcct ctggattttg ttaaggatct
    11701 cgagacgatc gaatacattg gcgataatca gtttgtcatt agcgatgagc gcgattacgc
    11761 catttatgtg atttcactga ccccaaactc ggaagtaaaa atcctcaaaa aaataaaaat
    11821 cccgctgcaa gactccccaa ccaattgcgg ttttgaaggg ttggcttatt ctcgccagga
    11881 tcacactttc tggtttttta aagagaaaaa tccgattgag gtctacaaag ttaacgggct
    11941 gttaagtagc aatgaattac acatcagcaa agacgaggca ctacaacggc aatttacttt
    12001 agatgatgtg tccggcgcag aatttaacca gcaaaaaaat acgcttctgg tgctgtctca
    12061 tgaatctcgc gcactccagg aagtaaccct ggttggcgag gttatcggtg aaatatcact
    12121 aaccaaaggt agtcgaggtt tgtcgcataa catcaaacag gccgaaggga ttgcgatgga
    12181 tgcctctggt aatatttata tcgttagcga accaaatcgt ttttatcgct ttacccctaa
    12241 atcatcgcat taacaggctg gcatatttca cctttttgtt attaacttgt taacaacaaa
    12301 aatgacatac aaggttaaac atgccttcgt cctcgcatcc cgtagaacgc ttttctttca
    12361 gcaccgcgct tttcgggatg ctggttctga ccttaggtat gggattaggt cgcttccttt
    12421 atacgcctat gttgcccgtc atgatggcgg aaggggcgtt ttcatttagc cagctctcgt
    12481 ggattgccag cggcaactat gcggggtatc tggctggcag tctgctattt tcgtttggcg
    12541 catttcacca gccgtcgcgc ctgcgcccat ttctgttggt ttctgccctg gcgagcgggt
    12601 tgttgatcct cgcaatggca tggttgccgc catttattct ggtgttattg attcgcttcc
    12661 tggcgggtgt cgccagcgcc ggtatgctga tttttggttc gacgctgatt atgcagcaca
    12721 cgcgccatcc ttttgtgctg gcagctttgt tttctggcgt tggcattggt attgcactgg
    12781 gcaacgaata tgttctggca ggcctgcatt ttgccttctc ctcgcaaacg ttatggcaag
    12841 gcgccggggc gctttctggc atgatgctga ttgcacttac gcttttaatg ccctcgaaaa
    12901 aacacgccat cgcaccaatg ccattggcaa aaacggagca acagataatg agctggtggt
    12961 tactggctat tctgtacggc cttgcgggat ttggttatat catcgtcgcg acttacctgc
    13021 cgctcatggc gaaagatgca ggctcaccat tgttaaccgc ccatctctgg acgttagtcg
    13081 gcttatcaat tgtgccaggc tgctttggct ggctatgggc agcaaaacgt tggggggctt
    13141 tgccttgcct gacggcgaat ttgctggtgc aggctatcag tgtgctgctt actctcgcca
    13201 gcgactctcc tctcttgctt atcattagta gtatcggttt tggcggtacc tttatgggca
    13261 cgacatctct ggtgatgact atcgcccgcc aactgagcgt tccgggaaat ctcaatcttt
    13321 taggctttgt gacgctcatt tacggcattg gacaaatcct cggcccggcg ctgaccagta
    13381 tgctcggcaa tggaacatcg gcgcttgccg gcgccactct ctgcggcgcg gcggcgttgt
    13441 ttattgctgc gcttatcagc acagtacaat tatttaagct gcaagtggtc acttcatgag
    13501 gagtgtttcc cttaccactc aagaaactca acagcaaccg ttgatgtcag ccagaccccg
    13561 ttttccgctt gccagaaagg gatgcctcgt ttcgccattt cctgtgcttt gacggttaag
    13621 ataaccggcg atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga
    13681 tgtacgtaat gacgctcccc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg
    13741 cttgccgtac cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatggcgacc
    13801 tgcgaagtcg aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa
    13861 cgctttttat cgctggtggc aaccacagta tccagcaatg cacgggttaa ccgcttgccc
    13921 gctttttggg cacaaagtat gagcttatcg atatccgccc atccttcacg gtcaagtacg
    13981 ataccaatcg cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc
    14041 aactcttttt cgttgtactt tgccatcgcg ttttcgtccc tggattttct gtttttattt
    14101 actgatgtca ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gcagagcctt
    14161 ttctacacag cgtaattttt ccgctatgca aaaaacgcat agcaagaaac atacttgcat
    14221 tttccccata cccacatggc tgataaggtt tttcagctcc aggaattttc ctggctcaag
    14281 caacacacaa acacaaaaaa acaatgggga ttgttatgac acaacaaggg gatgctgtag
    14341 ccggggagct ggcgacagaa aaagtgggca ttaaagggta tctggctttt tttctcacca
    14401 taatcttttt ttccggagtt ttttccggga cagacagctg gtggcgcgtt tttgattttt
    14461 ccgttctgaa cggatcgttt gggcaactgc ctggtgcgaa tggagccacc acctcatttc
    14521 gcggggcggg tggcgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt
    14581 cagtcattct ctctctgggg attatttcaa ttaccgatgg tcttggtgga ttacgcgccg
    14641 cgcagcagtt aatgacgcct gtgctaaaac cgttgttggg cattccaggg atctgctcgc
    14701 tggcgctgat cgctaacctg caaaacactg atgccgccgc cggtatgacc aaagagctgg
    14761 cgcaggaagg ggaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg
    14821 gtagcgccat catcaccaac tacttctctt ccggcgtcgc ggtgttcgcc tttctgggca
    14881 cgtccgtaat cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga gccaacattt
    14941 tgcgcgtctg gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac
    15001 aactcaggtc cgcaaaaacg tcatggatat gtttatcgat ggcgcgcgcc gtggctttac
    15061 tatcgccacg accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct
    15121 gaaaattacc ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg
    15181 gggattaccc ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggcggcgc
    15241 agtcggtgtt gccgccagtc tggcgaccgc cggcgcatta accggtcacg atgtcaccgt
    15301 tttactgccc gcgatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg
    15361 gacagccgaa gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc
    15421 attgctgtcg atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta
    15481 taccgcagcc ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg
    15541 aatttgcgat gtcctcgtcg ctaacggcaa aattatcgcc gttgccagca atatcccttc
    15601 tgacattgta ccggactgca cggttgtcga tctcagcggg cagatcctct gtcctggttt
    15661 tattgatcaa cacgtccatt tgattggtgg tggcggcgaa gcaggtccca cgacgcgcac
    15721 gccagaagtg gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg ttggtctgct
    15781 gggcaccgac tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa
    15841 tgaagaaggc atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcaccat
    15901 tacaggttcc gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg tgaaatgcgc
    15961 catctctgat caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcgga
    16021 atcccgcgtt ggcggtttgc tcggcggtaa acctggcgtc accgtgttcc acatgggcga
    16081 cagcaaaaag gcgttacagc ctgtctatga cctgctggaa aactgcgatg tgccgatcag
    16141 caagttgctg ccgacccacg ttaaccgcaa cgttccgttg tttgagcagg cgctggagtt
    16201 cgcgcgcaaa ggcggcacta tcgatatcac cagcagcatt gacgaaccgg tcgcccctgc
    16261 cgaaggtatt gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc
    16321 cgacggcaac ggtagccagc cgttcttcga tgacgaaggg aatttaaccc atatcggtgt
    16381 tgccggtttt gaaacgttgc tggaaaccgt gcaggtgctg gtcaaagact atgatttcag
    16441 tatcagcgat gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acctgaccgg
    16501 gaaaggcgaa attctgccag gcaatgatgc tgacttgctg gtcatgacgc cagaactgcg
    16561 cattgagcag gtatacgctc gcggcaaact gatggtcaaa gacggcaaag cctgcgtgaa
    16621 tggaacgttt gaaacggctt aaacggttgt cagccgcccg aatggacgta gaatgcccgc
    16681 aagggcggct gacagagtaa aacctaatgg atgactgtgg tgcggttttg cataatattg
    16741 aaaccaaatg gctttatgat tttctgaccc tggaaaaatg ccgcaatttt tcccaggcgg
    16801 cagtcagtcg caacgtctcg caaccggcat tcagccgccg catccgtgca ctggaacagg
    16861 cgattggcgt tgaattgttt aaccgccagg tgacgccact gcaactctcg gaacaaggga
    16921 aaatctttca ttcgcagatc cgccatctgt tgcaacagtt agaaagtaac ctggcagagc
    16981 tgcgtggcgg cagcgattac gcgcaacgta aaatcaagat agccgctgca cactctcttt
    17041 ccctcggtct gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcaattg
    17101 aagccattga tgttgatgaa gcggtcgata aactgcgtga agggcaaagt gactgtattt
    17161 tttcctttca cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat
    17221 ctcaattgtt ccctgtctgc gccagtgacg aacacggaga agcacttttt aacctcgcgc
    17281 agccacactt tccgttactg aattacagcc gcaactccta catggggcga ttgattaatc
    17341 gcaccctgac gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg
    17401 agcttttaaa gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gagtacgcca
    17461 tccaacaaga aattcgcagc gggaagctcg ttgtactgaa tcgggacgaa ctggtgatcc
    17521 cgattcaggc ctatgcatac cgaatgaata cccgcatgaa tccagttgcc gaacgcttct
    17581 ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca ttctccagca tcgcactaca
    17641 ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt tcaccgtcgt ggtacagcat
    17701 tatctcaagg ctgatcgcca gaatgtggca gtacgcttct ttaaaattaa cctgcaacgc
    17761 cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg
    17821 gagatgtttg tcgctctggt tatgcacttg ccactctggc atgcctgaaa caagtaatga
    17881 cggtaatgcc gaatgaggag aattaaaaag agtgaggaga tcgctgcata ccgatttacg
    17941 cagcaatgac gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg attggcgcat
    18001 cattttgcgc actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca
    18061 ctgtattgat gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct
    18121 gagacaggcc tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact
    18181 gacttttcag gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaccgct
    18241 agtatattct gaatattccg aatagttacg tagatgaaaa tacttgtcac cgctgctact
    18301 taattccggt gggaaattat ttatttccgc ataatcagcc atgtattatt tgaaacaatt
    18361 agttt
//
LOCUS       DAPHDX010000084        18357 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPHDX010000084 DAPHDX010000000
VERSION     DAPHDX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.084"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(119..691)
                     /locus_tag="RH058_002957"
     CDS             complement(119..691)
                     /locus_tag="RH058_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDV3138516.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(765..1046)
                     /locus_tag="RH058_002958"
     CDS             complement(765..1046)
                     /locus_tag="RH058_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV3138517.1"
                     /translation="MKRPKRMLVTGYCCRRCKGLALSRTSRRLSQEVTERFYVCTDPG
                     CGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQSYSLRLE"
     gene            1071..1385
                     /locus_tag="RH058_002959"
     CDS             1071..1385
                     /locus_tag="RH058_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HDV3138518.1"
                     /translation="MAVPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAIAA
                     ELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKAPV
                     LH"
     gene            1400..3733
                     /locus_tag="RH058_002960"
     CDS             1400..3733
                     /locus_tag="RH058_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV3138519.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGCLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWCAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQTKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQSGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            complement(4352..4690)
                     /locus_tag="RH058_002961"
                     /pseudo
     CDS             complement(4352..4690)
                     /locus_tag="RH058_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4741..5697)
                     /gene="paoD"
                     /locus_tag="RH058_002962"
     CDS             complement(4741..5697)
                     /gene="paoD"
                     /locus_tag="RH058_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDV3138520.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLMLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            complement(5707..7905)
                     /gene="paoC"
                     /locus_tag="RH058_002963"
     CDS             complement(5707..7905)
                     /gene="paoC"
                     /locus_tag="RH058_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDV3138521.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(7902..8858)
                     /gene="paoB"
                     /locus_tag="RH058_002964"
     CDS             complement(7902..8858)
                     /gene="paoB"
                     /locus_tag="RH058_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDV3138522.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(8855..9544)
                     /gene="paoA"
                     /locus_tag="RH058_002965"
     CDS             complement(8855..9544)
                     /gene="paoA"
                     /locus_tag="RH058_002965"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV3138523.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9962..10576
                     /locus_tag="RH058_002966"
     CDS             9962..10576
                     /locus_tag="RH058_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV3138524.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(10824..11153)
                     /locus_tag="RH058_002967"
     CDS             complement(10824..11153)
                     /locus_tag="RH058_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3138525.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(11466..12176)
                     /gene="ecpE"
                     /locus_tag="RH058_002968"
     CDS             complement(11466..12176)
                     /gene="ecpE"
                     /locus_tag="RH058_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDV3138526.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(12145..13788)
                     /gene="ecpD"
                     /locus_tag="RH058_002969"
     CDS             complement(12145..13788)
                     /gene="ecpD"
                     /locus_tag="RH058_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV3138527.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(13778..16303)
                     /gene="ecpC"
                     /locus_tag="RH058_002970"
                     /gene_synonym="yagX"
     CDS             complement(13778..16303)
                     /gene="ecpC"
                     /locus_tag="RH058_002970"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV3138528.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(16329..16997)
                     /gene="ecpB"
                     /locus_tag="RH058_002971"
                     /gene_synonym="yagY"
     CDS             complement(16329..16997)
                     /gene="ecpB"
                     /locus_tag="RH058_002971"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV3138529.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(17055..17642)
                     /gene="ecpA"
                     /locus_tag="RH058_002972"
                     /gene_synonym="matB"
     CDS             complement(17055..17642)
                     /gene="ecpA"
                     /locus_tag="RH058_002972"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV3138530.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            complement(17717..18259)
                     /gene="ecpR"
                     /locus_tag="RH058_002973"
                     /gene_synonym="matA"
     CDS             complement(17717..18259)
                     /gene="ecpR"
                     /locus_tag="RH058_002973"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV3138531.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
BASE COUNT         4019 a         4973 c         4944 g         4421 t
ORIGIN      
        1 agaccgcgcc gggaatggcg cggtctgcag aaaatgaggc agggaaacgc atgcaaagcc
       61 atgcacctta tcgatgcatg gcttttttca gtaaaaacgg gcggattttc ggggaatttt
      121 acacagactg acgtgatgcc agttgcgcac ttttacgcga aaaaatcatg ttctgcgcag
      181 gggtgaattt ttcacggctg tcatccaccg aagccgcgtc aggcctgaat ccgatggtcg
      241 ttaaaatgtc gctatcctgt gcggaataat taattttttc ccccttcgca agccaggaca
      301 gaagggcttc acgcagggca tctgtggcac gctgtatggc acagtttcgg gcaatggccg
      361 tcagctcact gtagcccatc agctccggtg ccagtgccgc cgccagtgct gtgccgtgct
      421 gctgcataaa atcattcagc cggtcgcgga tgctgatgtg ctgaacggct tcatgcgaac
      481 gaatataacg accggcggcc tgattcacct gccattttct gacttcgata atattgcgta
      541 attcgtccag gcggctgacg tttctgcctt ctcctgacag aagccgcaga tattcctgtt
      601 cggccgctgc cagctcattt ttgcgttgca gccatgctgc tttgttattc tgacaggtgt
      661 caaaggcctg ctgtaaggct gtgctttcca tcgttatctc tttctcatca tgctgaagaa
      721 taaaaatacg gtgtgcggcg acggccggtg ttaaccggca gccctcattc cagacgcagc
      781 gaatatgatt gtgtttttaa gcgtactggc ggcagttcct gtttttcatg caggctttct
      841 gccagttcgt ccggcgtgac cgggcggaca atgaagcggt tgatggtctg aagcgtttta
      901 aacaccagac cacagcccgg atccgtgcac acataaaaac gctcggtgac ttcctgagac
      961 agacgccgcg atgttcttga cagtgcaagg cctttacatc tccgacaaca atatccggta
     1021 acaagcattc ttttcgggcg tttcatactg ccggaggctg acgtcagtga atcgcggtac
     1081 cgcccgtctt acgcgctgcc ctttaccgtc gcgctgtcgc ctgtgcctgg ctgaccgtgt
     1141 gcgaacgtca gcaccgctac ccgcatctca cccttgagtc actggaggcg gccatcgccg
     1201 ctgagctgga gggcttttat ctgcgccagc acggtgagga aaaagggcgt cagatagcct
     1261 gtgccctgct ggaagattta atggaatccg gccccctgaa ggccgcgccg tcgctgtcct
     1321 ttctcgggct ggttgtgatg gatgaactct gtgcccgtca cataaaagcg ccggtactgc
     1381 actgaaggag aacaacacca tgaaaatgaa cgtaaccgcc accgtcagcc atgcgctcgg
     1441 ccactggccg cgtattctcc cggcgctggg gattcaggtg ctgaaaaacc gtcatcagcc
     1501 ctgtccggtc tgtggcggga gtgaccgctt ccgttttgat gacagggagg ggcgcggcac
     1561 ctggtactgc aatcagtgtg gtgccggtga cggcctgaaa ctggttgaaa aggtgtttgg
     1621 tgtttccccg tccgacgcgg ccgcaaaggt ggctgccgtg accggctgtc tgcccccggc
     1681 tgacccggca gtgacggccg ccgccggtgc tgaaacagac gctgcccgga agaacgccgc
     1741 cgcactggca caaaccctga tggcgaaaac ccgtcccgga accggtaacg cctacctgac
     1801 ccgcaagggt tttcccggcc gggaatgccg gatgctgacc ggcacacaca gagccggtgg
     1861 cgtgagctgg tgcgccggtg accttgtggt gccactgtat gacgacagcg gcgaactggt
     1921 taaccttcag ttaatcagcg ctgacggccg taagcgcacc ctgaaaggcg gacaggtcag
     1981 gggcacctgt cacacccttg aaggacagaa tcaggccgga aaacgtctgt ggatagcgga
     2041 gggatacgcg accgcactta ccgtgcatca cctgaccggt gaaacggtga tggtggcgct
     2101 ttcttccgtg aacctccttt ctctggccag ccttgcccgg cagaagcatc cggcctgtca
     2161 gattgtcctt gctgctgacc gtgacctcag cggtgacggc cagacaaaag ccgccgcagc
     2221 cgcagatgcg tgtgaaggtg ttgttgccct gccgccggtc ttcggtgact ggaatgatgc
     2281 cttcacgcag tacggcgggg aagccacccg taaggccatt tatgatgcca tccggccacc
     2341 ggctgaaagc ccgttcgaca ccatgagcga agcggagttt tccgccatga gtaccagcga
     2401 aaaggccatg cgtatctatg agcattacgg cgaggcgctc gcggtcgatg ccaacggcca
     2461 gcttctgtcc cgctatgaaa atggtgtctg gaaggtgctg ccgccacagg actttgcccg
     2521 ggatgtggcc gggctgtttc agcgtctgcg cgcgccgttc tcctccggga aggtggcctc
     2581 cgtggtggac accctgaagc tgattattcc gcagcaggaa gccccctccc gccgcctgat
     2641 tggctttcgt aacggcgtgc tcgacacgca gagcggcacg ttccacccgc acagtccgtc
     2701 acactggatg cgcaccctgt gcgatgtgga tttcaccccg ccggtggaag gggaaacgct
     2761 ggaaacccac gcccccgcgt tctggcgctg gcttgaccgt gccgccggtg gtcgtgcgga
     2821 aaaacgcgac gtgattctgg ccgcactgtt tatggtgctg gcaaaccgct acgactggca
     2881 gctctttctg gaggtgaccg gtcccggcgg cagcggcaaa agtatcatgg ccgaaatagc
     2941 caccctgctg gccggggagg ataacgccac gtcggccacc atcgagacgc tggaatcccc
     3001 gcgtgaacgt gccgcgttaa ctggcttctc actgatacgc ctgccggacc aggaaaaatg
     3061 gagcggcgac ggtgccggac tcaaggccat caccggcggc gatgcggtgt ccgttgaccc
     3121 gaaataccgg gatgcatact ccacgcatat cccggcggta attctggccg tgaacaataa
     3181 cccgatgcgc ttcaccgacc gcagcggcgg cgtgtcacgc cggcgggtga ttattcactt
     3241 cccggaacag atagccccgc aggagcgcga cccgcagctt aaggacaaaa tcacccgcga
     3301 gctggcggtc atcgtgcgtc acctgatgca gaagttcagc gacccgatgc tcgcccggtc
     3361 actgcttcag tcccagcaga actcagacga ggcgctgaac atcaaacggg atgccgaccc
     3421 gacgtttgat tttatcggct atctggaaac cctgccgcag accagcggca tgtatatggg
     3481 gaacgccagt atcatcccgc gcaattaccg taaatacctc tatcacgcct atctggccta
     3541 catggaggca aacggctacc ggaatgtact cagtctgaaa atgttcgggc tggggctgcc
     3601 ggtgatgctg aaggaatacg gactgaatta cgagaagcgc cataccaaac aggggataca
     3661 gaccaacctg acgctgaaag aggaaagcta cggcgactgg ctgccaaaat gtgacgaccc
     3721 tgcgacaacc tgaccatcat gactcatctg accggcatct gccggtcttt ttttatccct
     3781 gaattccccg aaggtgaaca atccactgtt cacccttcac cgtgtattca cccgttatca
     3841 cactgaaatt aaaagagaaa aacgaaaggt gaacagtgtg aacaatcaaa tcaaaaaaaa
     3901 cttttttttc tccctgtgtg atttcagtat gggggattaa tcaccggtat gagtcacacc
     3961 ggcagaatgc cggaggtgaa gaatcgaatg ttcacccttc acccattatt caccacctat
     4021 cactctgaaa taaaaggaga caaaagaaag gtgaacagtg tgaacagttc tttcgaaaaa
     4081 aaactttttt cttactttta attgtgtaaa aaacaagcga tgcgcgcagt gtgtatagat
     4141 atgtgtatag ttatattttt tgaatataat aatctattat aaatcaatgt cttaactttt
     4201 aatattcatt cctattaacg caccattaaa atcaaagagt taccccatat ttaaatacac
     4261 cacgtttcct cctgtgccgc atttgtgcca ttgtaacctt ggcaattcat caaaatactg
     4321 ttctgacatc aggcagtgca ggtgcagcta tttaaaccaa ttgctgccgc cattctttga
     4381 cgtagtcaat cagggcgcgg agctttggtg caatattgcg acgctgtggg aaatacagat
     4441 agaagcccgg aaattgtgga agaaagtcat caagcagcga tacaagctta ccgctttcaa
     4501 tatatggcct gaaagtttcc tgagtggcaa ttgttattcc tccgccggca agagccagcc
     4561 tcaacatcag acgcagatca ttagtcgtaa tctgcggttc aatcgcaagg tcgaaagttc
     4621 tcccgttttc ttcaaatggc cagcgataag gcgcaacctc cggggactga cgccagccga
     4681 tacacttatg ggtatttccc ccggaggcga gaaagcgctc tccacgcccg gccgcaagga
     4741 tcaggacgac gggggcaggc atgaatcctc ctcatgatgg agacgtacag aggcgatttc
     4801 tgccagcacg gagagtgcca gagtatgcgc atcccgggct ttggggaata tcccgacggg
     4861 ggcccggatt tgcgctgtct cctccctgga ccatcccagc tcgtggagct tttgcagacg
     4921 taacgtgtgg gttcgatggc tgcccaatgc gccgagataa aagggttttg cttctcgcgc
     4981 ggcctgcaat gctggcagct cccggttgag atcatggcac agcaaaatga ccgccgtgtc
     5041 ggtatcgatc tgagcgctgg ctgaggccgg aaaaagatcg aagatatggc tgtcatagcc
     5101 tgtggctgct gcaagactcg cggttgcctg cgcctcaaga gaacgtccgt aaatcatcag
     5161 cctgacgcat ggcctgaacc ccacctcaaa gccattgaga ttccagcccg ttcgggtttg
     5221 cgtgggcagg cacaccaacg attgtgcttg cggatcgtag cgcagccccg ccggttttct
     5281 ctgttccaga cggttcagca cggcgagcag aggctgtgcc gagcgtagtt tatggagcat
     5341 cagcgtgatc ccaccgccgc agggcagaac gatgtcaaac cacggcgaac cttcgccata
     5401 gcgaatttcg cgatcgcggc ctgagcccat catctccagc gcttcaaaag cggcagcggc
     5461 ctccacgcag ccgccagaga caaaaccgca gtaacgacca tcttcgcgaa ccaccatctg
     5521 cgccccgagc gggcgcgccg cgccgccgtg tatctccacc agcgtcacca gcaccgctcc
     5581 tttaccggac attagcgcct ctacggcgaa gcgcagaatg gtccggtggt catcggtgag
     5641 aaacgcctgc tctggcttat gccagtgttc gtctttatca aaaagcgggt atgacattgt
     5701 ttctccttaa accacatccg gcagcttatc gagcagctta tccagagtga tgggataatc
     5761 ccgtacccga ataccggtgg cgttatacac cgcgttggcg atagccgcgc tcacgccgca
     5821 caggcccaac tcaccgacac ctttggcctt catcggggag gatatggggt cggtatcatc
     5881 caggaaaatc acctcctgtt ttgggatatc cgcatggacc ggcacctcat accccgccat
     5941 atcgtgatta acgaagtagc ccaaacggtc atccaccgcc agctcctcca tcagtgccgc
     6001 gcccatgccc atagtcattg cgccaatgac ctggctgcgc gcagttttcg gattcaggat
     6061 gcgtcctgca gcacacacag cgagcatacg ccggacccga acttctcccg tcgcgctatg
     6121 cacgccgacc tccacaaaat gcccggcaaa ggtcgactgc tggtactctt tgctcagtgt
     6181 tccgaattca atgctctctt ccgctgtcag tctgccgcct gcggtggctt catgtagcgt
     6241 ggcgcttcgg gtaccgttgg taatcttgcc gtcggcaaac tgcgactgct caggatcaaa
     6301 cccgactgcc gaggcaatca tttcgcgaag cttcatacag gcggcgtaaa cgccggaggt
     6361 ggaggtattc gcgccccatt gtccaccaga acccgcagaa accgggaaac tggaatcgcc
     6421 gaggtgaacc gcaacctgct ccagcggtac gccaagcatt tccgctgccg tctgggccag
     6481 aatggtgtag ctgccggtgc caatgtcggt catgtccgtt tctacggtaa cggtgccgtt
     6541 ttgttcgagg tgaacccgag cacccgattt ttccagcaga ttattgcgaa agcccgccgc
     6601 aacaccgtgg ccgactagcc actccccgtc gcgcacctgt ccgggtgtgg cgttgcgctg
     6661 cttccagcca aatttatccg ctccggtgcg caagcactcg ataagctgac ggcgagagaa
     6721 gcagcgcgtc gggtcggcgg ggtcaacctg agtgtcattc aggatgcgaa actcgacggg
     6781 atcgatgccc gctttttccg ccagttcgtc gatcgcgatt tcgagcgcca tcagaccggg
     6841 ggcttcgccg ggcgcacgca tggcgttccc ttccggcaaa tcaagcgtgg cgagccgcag
     6901 gccggtatga cgattcgccc cggcgtagag taattcgctt tgctgtaccg ccgtttccgg
     6961 cgtgccgccg ggcaggtttc cagaccagct ttcatgtgag atagcggtga ttttcccgct
     7021 ctggtcggca ccgatacgca agtgctgaag ggtggcgggg cggtgcgtgg tgttattggg
     7081 aatagagggg cgggggagca tcactttaac cggacgtttc accgctcggg cggcgagggc
     7141 cgccagcagc gcatcgcttc tcaggaacag cttgccgcca aaccctccgc cgatatacgg
     7201 ggagataata cgcacattct ccacgggaac tttcagcgtt tttgccagat cggtgcggca
     7261 ccagtcaatc atctgatttg aggtccagag ggtaagctta tttccatccc agacggccat
     7321 cgaggcatgc ggctccatcg ccatatggct ctggtccggg gtcgtgtagg tagcatcaat
     7381 cttcaccgca gcggaggtga aagccccgtc aaagtcaccg acgtttttgt cgggcgtgtc
     7441 ttccggcggc tgattgacgg cctgtttttc gtccgccagg gagtaagctc ctttattacg
     7501 gcgatagtgt gcctgcacca gcgaggccgc cgctcgcgcc tgttcgaagg tctcggccac
     7561 taccagcgca atggcctgat gatagtgctc aatagtgggg ccgcctaaca gcctggcggt
     7621 gtttttgtcg cctttgccga gtgccccggc gttactggcg gtaatgacag ccagtacgcc
     7681 cggagctttt tgcgcggcgt ccgtatcaag ggcggtgagg cgtcctttgg caatggcgga
     7741 accgacgata tagccatagg cggcgttggg ggcctcttca tgccattcgt aggcgtagcg
     7801 tgccgtgccg gtagttttca gcggtccgtc gatgcggtca tggggacgac cgacaacctt
     7861 cagctgatcg atcgggtttt cccctgcggg tttatcaaat ttcatgcctg tgccctcgct
     7921 tcagccagta cggaggcaag cgttcgcttc gccaacagga gtttaaaggt gttttcagcg
     7981 gtgggatggg cgctggcgaa cagcgtgtca tatacggcct gcgccccctg ggatagctga
     8041 gcatccgcag cctcaatgcg ccagggctta tgtgctactc cgcccagcgc gacgcgcccg
     8101 ctgccgtcag gctgaataat cgccgcgacc gataccaggg caaaggcgta ggaggcgcga
     8161 tcgcgcacct tacggtagat atgttttccg ccgagcggtg gaggtaacgt caccgcaacg
     8221 ataagctcac cgggaagcag ggcggtttca atgtgcggcg ttttccccgg tgggtgataa
     8281 aaatcagcca gtgtgatact gcgagtcttt ccctccggcg tgatggtttc caccaccgca
     8341 tccagcaacc gcattgcgac cgccatatcg ctcggatggg tggctatgca ggcttcactt
     8401 acgcctacca ccgcgtgctg acggctaaag ccttcaagcg ccgcgcagcc gctcccgggc
     8461 aggcgcttat tgcagggctg attggtgtcg taaaaatagg ggcagcgcgt gcgctggagc
     8521 agattacctg cggtggttgc ctgattacgt aactgaccag atgcgccagc gagcagggcg
     8581 cgggagagta ccgcgtaatc acgacgcacg cgctcgtgag ccgccaggtc ggtgttccgt
     8641 accagtgcgc cgatgcgcag cccacccgcg tcggtcactt caatcttatc gagcccgagg
     8701 ccgttcacat cgataaggtg ggtgggcgtt tcaatttcca gcttcatcag gtccagcaga
     8761 ttggtcccgc ccgcgataaa ttttgcgccg ggtacgcgct gagcgctaag tgccgcctcg
     8821 gctggggtgt tcactcgttc ataggtaaac gccttcatga ttttatctcc cccgcagcat
     8881 cttcaatggc ggcaaggatg ttagcgtatg caccacagcg acagatgttg ccgctcatac
     8941 gttcacggat ctcatcggca gttgtttctg gagcggaaac caaatcgacc gtgacgtgac
     9001 tgggaatgcc gtcctgaatc tcttttagca ccgctactga tgagcaaatt tgcccggagg
     9061 tgcagtagcc gcactggaag ccatcatgct tgataaaggc cgcctgcatg gggtgaagat
     9121 tatctggcga acccaggcct tcaatggtgg tgatctcggc cccctgatgc atgactgcaa
     9181 gcgtcaggca ggcattaagc ctgcgaccat tgaccagcac ggtacaggct ccgcactgtc
     9241 cgtgatcgca acctttcttg gtaccgatca aatgcagatt ttcacgcaaa gcgtccagta
     9301 gcgtggttcg ggtatccacc tcaagctgct cggttttgcc gttcaccttc agtgtcaggg
     9361 gcattatctc tggcgcgggt gtagctgccg gaacgcttgc cgccagcgta gaatgaggat
     9421 aaaccacggc ggtcgccgct gttgcggcgc tcactttaat cagatcgcga cgggtcaaac
     9481 ttaaatcgtg cggctcgtgc ttcccaaccc gattatcttc ggggtattcg ccttggttgc
     9541 tcataccagg cctccggtat tctaaagggg aaaataagag tgcgttgcga gagtgctgat
     9601 gcttttttaa atgttaagca tagtcggcga cagcaggagg attattctta aaacgcgaat
     9661 gcagctatga gcccggctaa taaattcatg ttcgccggga tgttgatgat gatgggagct
     9721 ggtttattgc tttagttgta cgatgcaaaa accaataagg aaacctgtga ttttcagctc
     9781 tgcatcaccc tgcaaatctc tgtcacttct aatataaaaa tagggagaaa tgatggagct
     9841 tatattcatt ggcgattagg aaaccatctt gttatacaaa acaatacagt tctttacatt
     9901 tgccttgttt tatgaatact cctgaagagg tgtataacat aatggtacaa gcagggtaga
     9961 tatgaatata tttgaacaaa ctccaccgaa ccgcagacgt tatggtcttg ctgcattcat
    10021 tgggctgatt gctggcgttg tttccgcatt cgtgaagtgg ggggctgaag ttccattgcc
    10081 gccacgtagc ccggtggata tgtttaatgc agcgtgtggc ccggaatcat taatcagggc
    10141 tgcaggccaa attgattgct cgcgtaattt tctcaatcca ccgtatattt ttcttcgaga
    10201 ctggttgggg ctgacagatc ccaatgcggc tgtttatacc tttgccgggc atgtctttaa
    10261 ctgggttggt gttacgcaca ttatcttttc gatagtgttt gctgtcggtt attgtgtggt
    10321 cgctgaagta tttccaaaaa ttaaactctg gcagggctta ctggcaggtg ctttagccca
    10381 actttttgtt catatgattt cattccctct catgggactg acgccacctc tgtttgatct
    10441 cccgtggtat gagaatgttt ctgaaatttt tggacattta gtctggttct ggtctattga
    10501 aattattcgc agagatttac gaaacagaat tactcatgag ccagaccctg agatcccttt
    10561 aggctcaaac agataatgca ttgaatgata aaaatggcgc aaatacagcg ccatttttat
    10621 aggttaaaaa cattgctttt tatattctga tgcagatagt cagtgagtat atcgcgctac
    10681 ttcaggatga tgtagatccg aagaacgcta cagaagagag gcattgttgc tggcaaatag
    10741 aagaagtatc gggttttgtt acccctgaaa aacgaagccc cgctattatc gctggcgggg
    10801 cattgcaatt aattattgta tgtttataac ggtgtcagcc cgtatttagc ccttgcacga
    10861 ttgcaagcct cattgacgac tccgttctca ccagacatgg cgaattctct gcatgtggac
    10921 gatcgatttt tatatatcgt gcagcaggca tttttgcccg gggttcctgc aagggcaata
    10981 catcgggggt ttttctgatt ggtaccgctc atgcatcggt gaaaagggga tatttgttca
    11041 gtgagcctgg cgggaatagt accgccagca tcgtcggctt cggcccagta aaaagagacg
    11101 cggaaaaatg cacaacaggc accacacgtc atgcatggat tcagattgct cataattcac
    11161 cagtacagct aaaaatcgta aagagaacag cggtacatcg tatgtaagaa cgtaccgcgg
    11221 ttggctgggg aactttcgat agtgtgagta ttgaatgatt tccagccgtt accgatttta
    11281 cgtactaatt cgtgattaaa ccactcgtta gcaggctgcc tgataccttt ccgtgtctcc
    11341 tgaacactat ggtgtgcata tgagaaagcc catcgttaca ggtgatattg acgatcgctt
    11401 ccacatcatt taaacgattt ttggggctct gaggtggaat tcttccctcg accgatggat
    11461 aagtcttagt ccgccgaagg gggcttagcc ggacaggaat cgctaatctt aatgaatttg
    11521 tcgttataga ccagataatg attccccggc tgacgtaact caggctgatg aaccacgtct
    11581 cccggacgta gataccaggc atcgccttcc tcttcggtcg aatcacatcc tggcttaatc
    11641 agtagcttaa accatgtgtt gccagtgtta cttaccgttc ctgtcacctt gtcgaaggac
    11701 catttaaact gaacctgacg tggtcgtact accagaatgg tatccatcac caccaccggc
    11761 tccgtgctga cctcgccgcc ggttgggcta cgtcttgtct ggttacgagt ggggacttcg
    11821 cgaaatgaga cccggtagta gcgctcgcgg ttatcccgtg gaccatgata gtaaaattta
    11881 aaatactcgc tctcaccagc ctgcaacgcc agttggcggg gggcgaaaag cagttcacca
    11941 tccaccggtc gggtgcgcaa ttcactgctg cccgggctat caatagcact gatggcaata
    12001 cggtatatcc gtgcgctttt gttgttattc actacacgtt tgctgacaaa gtcagtctcg
    12061 gacggcagcg aaaatgtcag attgccgacc gaaatcgcct gtgtcaaagg tgccgccagc
    12121 atacagaccg ctgtcagcag ggctttagtt aatgttacgc cacgtcgcct gaacatgaat
    12181 ctctcctgaa gcgctgactt cgccaaacca gccgttatca tctaccgtac gcagcgaaat
    12241 ggcgttatcc attggaatcg aaaatttgac tgtggtctta tccatctgcc ccacttcgcc
    12301 agagatatca gtccacggtg tggtcaacca cagcgcatcg gtcatatccc gccagctatc
    12361 atcgcacccg gcatcgtagg tttttgtagc tccgttgcgg gtaataaagg aaagtgtcgc
    12421 cgggaacggt actttcgcct taccgtcatc ggagctgaag acacaatagg agcgcccgcc
    12481 aatcacctgc gcgggtccgg tcaccttgat cagcacttcg tcggcagctg ttttaccgct
    12541 ggtggttacg atatagccga aatcgagtgc cgactcgccg ctgccaacat atccctcccg
    12601 cgacggtgtc tgagtatatt catcggagat aatactgatg ctgaaatcac ggggtttgat
    12661 aatcagcgtg ttggaggtag aaaattcata ccagccagac tccggtgatg tggtgttctg
    12721 aaagcgaaag ttgaatagat ctttggtgtt gatatcgctg atcccgaggt tcaccatctg
    12781 cttaaagaag ttgctcgaga agaaaacata gatcgagtct gcgctcttca tctcgttgaa
    12841 ggtgtagtaa taggcggtat tgctcaccgg tttccatgaa ctgccattca gactgaactg
    12901 catatcgtac gccccgacgg ccgaggctaa cgacgagttg gcgatcgccg ggaatatatg
    12961 gattgaggtg ttgctgagtc cgtttgtttg cagggtatag ttgaccatct tacaacttaa
    13021 tcctgaaagg ctgccgatgg tctgtgtccg gcagtcggcg ttcccttcgc ccagagtcgg
    13081 tactccgtcg ctgttgataa atacctccgc cagcgagtgg gtatttatta accgcagatt
    13141 tgctgctttc gtatgggtga cgttgcgaac ataccagttg ccggtggcct gatccttaca
    13201 gcgtgcgccg ctgctggcgt catagttcac agaggtctgg caggcattga tggtcattgt
    13261 aaagctgctg ccgacgggca tttgttgcag atactggtaa aacgcgtctg acaacatgcc
    13321 gtgcatccac ttcgcgccgc cgctggtcac cgtcgcgcca taaaatccac tggcgtcggt
    13381 ggtttgcggc aggataagac tggtggtcat attacaccca gcgtaccagt tgatgcagcg
    13441 caagccagtt aaaggagatg aaactggtga attttccagc cacatatcaa acttccagtt
    13501 ggtataaagg ccggtgttgt aaccgttatc aatgtagccg aggctttgct gatagatggt
    13561 tcctgaacca gtgtatttta aaccggtcca gcggttggca ccggtcaggc gcggatccag
    13621 cgccccgcca ggagtgacga aaaagttgtc gtctgagttg ttttcgacaa acacaaactc
    13681 gcgtgccggt gcatccgccc aggttgtttt gctcactgcc gctctgagct cagttactgg
    13741 ccagattagc gcgaaaatta tcatcgttat tagtaggtta actctcattc tcttctcctg
    13801 tctgcgtcac cgccgcccac gatgagaggc cgctgcagac cacatcaccg acccagacgg
    13861 caccgcgcgc ctggttgagt tccagtgcca cttcgcaggt tttattgcca ctgtagcgaa
    13921 aatcgatagt gggatatttc ttatccacgt ccatgacaaa ctcgccgttt tcatcggttc
    13981 gggttcggcc gatatggttg ttaatccgtg cgttagccag cagtgtgccg tcttccgcac
    14041 ggatacgacc ggagacggta accatctgct tcacctctgg ctcaatgaca gcgacattgc
    14101 ctggatagag agtcagatgg cttttgcggc cgctgacgat atcgtagctg tcgagtgagt
    14161 ttttgctgtt ctgtaactcc acctcatatc ttccataggg agagagcggg agatagttac
    14221 gcttgccgtt aagcgggaaa atccgcccgt tgattttggc gctgatctga ccgtcgtcct
    14281 ccagcccggt gttgaatatc accccagcgt tgccatcagt ccgcccgctg gcagcgatgt
    14341 ttttaccctg ccagccgacg ctgccattgg cggtcaagtt ggtattgatg tagccgtccg
    14401 ccgcgctatt gacgttcagc gttccgctgg cgtaacgagc gtcgaactgt gcatacgccc
    14461 caccgctgag ggttttgtca tcaccggtat cgccggagat ggcacgtgac agattggcac
    14521 caacagtgcg aatggttcct tcatcaaact gtttgcgtgc tgacaggttt gccatggtgt
    14581 agccgttttg atgggtcatc cctgcgctaa accagttgcc cagtggtagc gagagatcga
    14641 gagcgatata tttccctgta ttggcgttgc tgtcgccgtt gttatagcgc tgaataccgg
    14701 cccgcaggcc aagcgaacca aaggtaccgc tgtagacatt ttgatagtaa tctgccgtgt
    14761 aataatggct gttgtaacgg cggtcatcat tgtagctgat gctgaatgtg cccagcttcg
    14821 accacagtga gttcaggttg agtgtgccgc cgattgcacg gttgtcggca tcgctacgtc
    14881 gcaattgatt gccaatgcgg gttttttcct ggttaaccca cagcgaacta aagcctcccg
    14941 gtagagtggc gctgatgctg ccgatgctgc tccatgagct gtcactggcc agcatatttt
    15001 gcaggttaac gttgatcgcc cccccaagcg gcagcgtcag acgggtttca cccaccgcct
    15061 gattatcgta tccatatccc gttgccgccc agctaagcgt actcaatgag ccggaggtcg
    15121 aggcacctgc tagccaactc tctttagctg gtcgcgtctt tttcccgttt tccgaccagc
    15181 gatccatatg aaagctaccg ccccatacct gccacgccag tggtgcaccg acgccgcgcc
    15241 cccggctaaa cagcttattg acccgctggg ggcgtttgct gatcacgcga ccgttaacga
    15301 tcacctcaac ttccacatcg taaatcccgt acggtagacc ccgggtatcc acttcatgat
    15361 tgcccatagt gaagttctga acgcttagta gccgcccatc acgggtgaga tgtacttcgc
    15421 ccgccgccgg taaaaaggcg atcactggcg tggctgactg gctgctgtcg aagatggtgg
    15481 agctggcctg gtttccccag gaaaggccgt aaatcttccc tgctgaaatg gcggtcatcg
    15541 gccctaagga ctgcaagttc caggtgtcga gcattccacc ggcaaatcgg tgaccggcaa
    15601 aatcgcgttc atacatcgct ttatataatt cactgtcctg ttgaccgcta ccgatcccgt
    15661 acagcgagcc gtcgagcacc acatgatgtt cgcgcagcgc agtaacgtta ttcagcgaca
    15721 gatagctgga tgtgttgctc ccgccgttac gcaactggtt gttatagacg cccaagttat
    15781 agctcagatt actgctgagg gtgttaacac tggactgccc gatgtcttcg ctacgtgagc
    15841 gtagtacggt gcccagcgct tcgcgcttga ccaccagctg cagcagcagt tggcgcaagc
    15901 tgagatccag ctgcgcgttg tcagtcaggg ggatggtaag ggcctcattg aacggggcgt
    15961 tggccagagc catcagctgc tgtcgagttt gttcgctgac actggcgtta tcttcactct
    16021 cttccagctg tattttccgg atgcgtagct gtccatcatc cagccagata aaagcgctgc
    16081 cgattcgctg atcgtcctgg cgaccctggc taccggcgag atgaatatag agcgggacgc
    16141 tcatgccgtc ctgaagcgcc tgactgaagg cctgcggaat aatcacccca cctatttgct
    16201 gcgcacttat gtcagcagcg ctggcatcgg gttgaacgaa caaaaagacc atgccgaagg
    16261 caaactgggc tttcagtcct ggggagaacc gtcgtaaagg cattgtcgtt atccatctgc
    16321 acagctatct atttcacggg aatgaactta tcaccctgcc aaagtgcaac ccgtcctttg
    16381 ttatccgcag tgtccacacg ggtaaaacga cgcgacttgc ccggcatcag gtagtaattc
    16441 tctttacact ccttaccgtt ggcggctttc aggcaagggc cgtaggcgag gatccgcagc
    16501 gtcgcatttc ctgtatttgt cagggagccg ttggcgtact gaaagtggta gttcgcctgg
    16561 cgaggggcga cgaccagaat ggtgccgatg cgggcggaag cagtggccac agcgctgcgg
    16621 ttggcattat cgcgctgcgc atcactgagg gcctgatcaa accagacaat gcggtagtag
    16681 cgctcttttt catctgcggg tcccttatag aagaagcgga tcacttcgct ggcttgggcg
    16741 ggtagcagca agctggcggg agtgagtagc aactcatccg gcttgtccat tgagataacc
    16801 tgcccgtcgt caagcggtga agagagccgt tcgagacgga tattgataag gcgaccactg
    16861 tcggtactgt ttttgatcgt tttgctcagc gtgctgctgt cactgttcat aaacgatgat
    16921 atatcgccga catccagcgc ctgggccgga gatattccgg aaaacagcag agcgagaggc
    16981 agaaggtgct ttttcataac aattccgtcc aggaataaag caggggggct acccctgctg
    17041 gtacatcaga gagattaact ggtccaggtc gcgtcgaact gtacgctaac gtcgccgctc
    17101 cagatgcctt ccggtagagt gctgtaatct gttactgcgg tggtaccatt ggtggtaccg
    17161 ctgatgatgg agaaggtgaa accatcctgt gcggtggtac gattgctggc attgtaaccg
    17221 ttagccagcg ggctaaggtt gccgcccagt acgccgttgg cggtatcgat catcacggta
    17281 tcgccagttt tttcgactgc cgcgccgtta taatccacgc ccacattcag tgtggaacct
    17341 gaggtatcca actgggttaa tgtgttggtg ataagacgtg aggtcagttt aaaggcggta
    17401 gccgttgagt caccctcgat agccacgtca aatagacctt tctgtgagtt aaaaccttta
    17461 atgccttcgg catactggaa cgccaggcta ccgagtggcg tcacaaccag cttactggtg
    17521 gtgtcttttt tggctgttgc cgaccaggtc gctacagcct gagctgttac gtcagcagcc
    17581 tgcgccacac ccatgccggt aaacaccgtt accagagcta ttgccagaac cttttttttc
    17641 attgcatttc ttcccgagtt gaattgagga cgtgatgtcc cagtgagtca ttttttaaaa
    17701 ctaacttgcc tggagtttac tgaaccaact tatatatttt tgagtacagc ttggcctcag
    17761 cattacgccg atgggtatac actgtcttca cactacaatt ttcaattctg gcaatcgatt
    17821 taggttgcat tccctgggcc gtcatgcgga taatttccat ttcccggtcc gtgattttat
    17881 ctttcttaat atcttttctc aggaacctgc cattaatcac ataggccagt tcttttctaa
    17941 tatcacgact cagtcctgcg tataccacgc ccctaatatt gctgttgtaa taccaataat
    18001 ttgctaaggc ttctgacttt ctggccgcaa tcaatacgac ctgtttcccc tttaccgcgg
    18061 ataaccattc attatcacgg tttataaatt cagataaaga atccgtattg agattgataa
    18121 acacgaaatc ttttctaatt ttctccaacg atagataaat taatctgtcg atatccacaa
    18181 tcagttcaga tagtccttta tagaagtagg cgtcatgggg agaccagatg tatttatcag
    18241 aacggttttg acattccata tggtttttaa cctcatagtc cctgctgtaa tcactttgcc
    18301 atgtcactac tttccaaacc tgtaattttt acaggtgcat tcagtttcct tagtaaa
//
LOCUS       DAPHDX010000085        18340 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPHDX010000085 DAPHDX010000000
VERSION     DAPHDX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.085"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            112..1332
                     /locus_tag="RH058_002974"
     CDS             112..1332
                     /locus_tag="RH058_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3138532.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWFRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1305..1934
                     /locus_tag="RH058_002975"
     CDS             1305..1934
                     /locus_tag="RH058_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV3138533.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1935..3095)
                     /gene="ybdL"
                     /locus_tag="RH058_002976"
     CDS             complement(1935..3095)
                     /gene="ybdL"
                     /locus_tag="RH058_002976"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV3138534.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3204..4292
                     /gene="hcxA"
                     /locus_tag="RH058_002977"
     CDS             3204..4292
                     /gene="hcxA"
                     /locus_tag="RH058_002977"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV3138535.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4302..4499)
                     /locus_tag="RH058_002978"
     CDS             complement(4302..4499)
                     /locus_tag="RH058_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV3138536.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4681..6786)
                     /gene="cstA"
                     /locus_tag="RH058_002979"
     CDS             complement(4681..6786)
                     /gene="cstA"
                     /locus_tag="RH058_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV3138537.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6967..7380)
                     /gene="entH"
                     /locus_tag="RH058_002980"
     CDS             complement(6967..7380)
                     /gene="entH"
                     /locus_tag="RH058_002980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV3138538.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7383..8129)
                     /gene="entA"
                     /locus_tag="RH058_002981"
     CDS             complement(7383..8129)
                     /gene="entA"
                     /locus_tag="RH058_002981"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV3138539.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8129..8986)
                     /gene="entB"
                     /locus_tag="RH058_002982"
     CDS             complement(8129..8986)
                     /gene="entB"
                     /locus_tag="RH058_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV3138540.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9000..10610)
                     /gene="entE"
                     /locus_tag="RH058_002983"
     CDS             complement(9000..10610)
                     /gene="entE"
                     /locus_tag="RH058_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV3138541.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTRQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10620..11795)
                     /gene="entC"
                     /locus_tag="RH058_002984"
     CDS             complement(10620..11795)
                     /gene="entC"
                     /locus_tag="RH058_002984"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV3138542.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPQQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11984..12940
                     /gene="fepB"
                     /locus_tag="RH058_002985"
     CDS             11984..12940
                     /gene="fepB"
                     /locus_tag="RH058_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV3138543.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12944..14194)
                     /gene="entS"
                     /locus_tag="RH058_002986"
     CDS             complement(12944..14194)
                     /gene="entS"
                     /locus_tag="RH058_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV3138544.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14305..15309
                     /gene="fepD"
                     /locus_tag="RH058_002987"
     CDS             14305..15309
                     /gene="fepD"
                     /locus_tag="RH058_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV3138545.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15306..16298
                     /gene="fepG"
                     /locus_tag="RH058_002988"
     CDS             15306..16298
                     /gene="fepG"
                     /locus_tag="RH058_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV3138546.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16295..17110
                     /gene="fepC"
                     /locus_tag="RH058_002989"
     CDS             16295..17110
                     /gene="fepC"
                     /locus_tag="RH058_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3138547.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17107..18240)
                     /gene="fepE"
                     /locus_tag="RH058_002990"
     CDS             complement(17107..18240)
                     /gene="fepE"
                     /locus_tag="RH058_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV3138548.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4328 a         5197 c         4726 g         4089 t
ORIGIN      
        1 atttattcac agcgcgaatg aaaaataaaa cactctgctt tacagtgttt actttataat
       61 taacagactt atctcactga tcaccctgta acgttcagag agcgtcttca gatgtctatt
      121 tataaaattc ctcttccgct caatattctg gaagctgcac gagagcgtat tacctggaca
      181 ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg gcaaagactc tggtttaatg
      241 ctgcatctga cagccgaact tgcccgacaa atgggcaaaa aaatctgcgt tttgtttatc
      301 gactgggagg cgcaattctc ttgcaccatt aactatgttc agtccctgcg cgagttgtac
      361 accgatgtca tcgaagagtt ttactgggtt gcgctcccgc ttacgacgca aaattccctt
      421 tcacaatacc aacccgaatg gcagtgctgg gaacctgatg tcgaatgggt acgtcaacct
      481 ccccaagatg cgataaccga ccctgacttt ttctcctttt accagccagg catgaccttc
      541 gaacaatttg tacgtgagtt tgccgaatgg ttttcacaaa aacgtccggc ggcgatgatg
      601 atcggcatcc gtgcggatga gtcctacaac cgttttgtcg ccatcgccag tttaaataaa
      661 caacgttttg ccgacgataa accctggact acggccgcac caggcggtca tagctggtac
      721 atttacccca tttacgactg gaaagtggct gatatctgga cctggtatgc aaatcatcaa
      781 agtctctgca acccactgta taacctaatg tatcaggcag gcgttcctct gcgtcatatg
      841 cgaatttgcg aaccttttgg cccggagcaa cgacaaggat tatggctcta tcacgttatc
      901 gaaccggatc gctgggctgc tatgtgcgca cgagtcagcg gggtaaaaag tggcggcatt
      961 tacgccggac atgataatca tttctatggg catcggaaaa tcctcaagcc agaacattta
     1021 gactggcaag aatatgcatt attgctgctc aatagcatgc cggaaaaaac agctgagcat
     1081 taccgcaata aaatcgccat ttatttgcac tggtatcaga aaaaaggcat cgaggttcca
     1141 caaacccagc aaggggacat tggcgcgaaa gatatcccct cctggcggcg gatatgcaaa
     1201 gttttactca ataacgatta ctggtttcgg gcattatcat ttagtcctac gaaagcgaag
     1261 aactatcagc gttataacga acggataaaa ggaaaacgtc aggaatgggg gatactatgc
     1321 aacaacgatt aacgcaggat ttaacgcaat ttctcgccag cttgccggaa gatgatcgca
     1381 tcaaagcgat taatgaaatc cgcatggcga tccatcaggt tagccccttt cgtgaagagc
     1441 cagtggattg cgttctgtgg gttaaaaaca gccaacttat gcctaatgat tacaacccaa
     1501 acaacgtggc accacccgaa aaaaaactgc tgaaaaaatc cattgaaata gatggtttta
     1561 cccaacccat tgtggtcacg catacggata aaaatgccct ggaaatcgtg gatggttttc
     1621 accgccatga aattggtaaa ggctcatcat cattaaaatt acgcttaaaa ggttatttgc
     1681 cggtgacctg tctggaaggt acgcgtaatc aacgcatcgc cgcgactatt cgtcataatc
     1741 gcgctcgtgg acgccatcaa atcaccgcca tgtcggaaat cgtccgtgaa cttagccagt
     1801 tgggatggga cgataataaa attggcaaag agctaggaat ggacagcgac gaagtgctgc
     1861 gcctgaagca aattaacggc ctgcaagagc tgtttgccga ccgtcaatat tcccgcgcct
     1921 ggacggttaa ataactaaag ctggcgcagg cgttcagctg ctgccagcaa cgtcgattcc
     1981 ttcttggcaa aacagagacg aatcagttta tgtgggaagg gatcggcgca aaacaccgac
     2041 agcggaatcg ccgctacgcc gtgctcctgc gtcagccact ggcaaaactc aacatcatcc
     2101 agggtagaaa cctcgctgta atccaccagc aaaaagtatg taccttcaca cggtaaaatc
     2161 tccagccggc tttcatttaa agcattcacc agaatatcgc gcttctggcg ataaaagtcc
     2221 ggtaacgcaa gataatgctc aggttctgca cgtagcatat cagcaagcgc cagctgtgcc
     2281 ggggtattca ccgaaaaggt cagatactga tgtaccttgc gaatttcggc gctgatgggc
     2341 gctggcgcaa cacaataacc cactttccag ccggtcatat gataggtctt gccaaatgaa
     2401 gaaaccgcca ctgcccgctc acgcagctgc ggatgcgcca gcacactggc atggccctgt
     2461 tgtgaaaagt tgatgtgctc gtagacttca tcgctaatga caaaaatctc gtgcccggcg
     2521 atcgcctgcc acaaagcggc gaaatcagcc tgctgccaga cagttgcact ggggttatgc
     2581 ggagtgttga ggatcaccag tctggtgcgc tcgcttaata atgcggcaaa ttcctgccag
     2641 tcaacgcgaa aatgcggtgg ttgcagtgcc atacgcttca ctattccccc agaaagcgcg
     2701 atggcggggg agtaactgtc atagctggga tcaaaacaaa tcacttcatc gccattgcgc
     2761 accagtgcgg taatcgccgc gtataacgct tccgtcgccc ctgccgttac ggtgatatcg
     2821 ctatcggcat ctggttgata gccatacaaa cgttccgttt tctgagcaat cgcctcgcgc
     2881 aaggcctgca cgccggtcat gggcgcgtat tggtttgccc cctgggcaac gtggtacgcc
     2941 agccgctcct gtaaatagcg cggaccatca aaatcaggaa agccttgcga caggttaatc
     3001 gcctggtgtt gctgcgccag cgcgctcatc tgggtgaaaa tagtggtgcc aagttgtgga
     3061 agtttgcttt gtggaatcag agggttattt gtcattttta ttgtacctga cgaagttgcg
     3121 tgttgaaggc actataacac gatgctagta tttggcaatc aagacgttta gatgtctaaa
     3181 taaaacaata aggacaacac aacatgcctc acaatcctat ccgcgtggtc gtcggcccgg
     3241 ctaactactt ttcacatcca ggaagtttca atcacctgca cgattttttc actgatgaac
     3301 aactttctcg cgcggtgtgg atctacggca aacgcgccat tgctgcggcg caaaccaaac
     3361 ttccgccagc gtttggactt ccaggggcaa agcatatttt gtttcgtggt cattgcagcg
     3421 aaagcgatgt acagcaactg gcggctgagt ccggtgacga tcgcagcgtg gtgattggcg
     3481 tcggcggcgg cgcactgctc gacaccgcga aagccctcgc ccgccgtctc ggtctgccat
     3541 ttgttgccgc tccgacgatc gccgccacct gcgcagcctg gacaccgctc tccgtctggt
     3601 ataacgatgc cggacaggcg ctgcattatg agattttcga cgacgccaat tttatggtgc
     3661 tggtggaacc ggagattatc ctcaatgcac cgcaacaata tctgctggcg gggatcggtg
     3721 acacgctggc gaaatggtat gaagcggtgg tgctggctcc gcaaccagaa acgttgccgc
     3781 taaccgtgcg actggggatc aataatgcgc aggccattcg cgacgtattg ttaaacagta
     3841 gcgaacaggc gctgagcgat cagcaaaatc aacagttaac gcaatcattt tgcgatgtgg
     3901 tggatgctat tattgctggt ggtgggatgg tcggtggtct gggcgatcgt tttacgcgtg
     3961 tggcggcggc tcatgccgtg cataacggtc tgactgtcct gccgcaaacc gagaaatttc
     4021 tccacggcac caaagtcgct tatggcattc tggtacaaag cgccttgctg ggtcaggatg
     4081 atgtgctggc gcaattaacc ggggcgtatc agcgttttca tctgccgacc acgctggcgg
     4141 agctggaagt ggatatcaat aatcatgcgg agatcgacaa agtgattacc cacaccctgc
     4201 gtccggtgga atccattcat tacctgccag tcacgctgac accagatacg ttgcgtgcag
     4261 cgttcgaaaa agtggaatcg tttaaagcct gacgtgcaaa tttagcagca gcgcgcgccg
     4321 ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa actcttcgta ggtcatcggc
     4381 gtttgatcgg gatggttaac ccgcatatgt tcgacatagt tgtcgtaatc aggcatacca
     4441 atcatcagct tcgccgcctg acctaaatat tttccggctt ttgccagtga atcaaacatc
     4501 ttggcctccg ctaaccatgt tgctgaaaga gtgccggatg cggcgtgaac gctttatccg
     4561 gcctacaaat gctatcaacg cactcggtca gtcccctcgc cccatcgggg agagggttag
     4621 ggtgaggggg aaccctcggc actatgccaa cattcgccaa catcccccct cactctgact
     4681 ttagtgcgca cctttcgcct gcgctacgat ctcctcaaca ttttccggca ttggctcata
     4741 cggcgtttct ttcgccgttg gcttcggctc tttcaatgcc gcaagtgccg tcttaatcga
     4801 gaacagtgcc agaaccacca cgaccaccat aaagaagatg gttaacccgg catccagacg
     4861 gttgttgaac accagttgcg ccagctgtga ctcagtatac tgcgacggaa tattgccgct
     4921 gtcgatcatt gcctggaact tgttagcaat ggccaggaag ccgactttcg catccgggct
     4981 aaacgctttc tgccagcctg cggtcagggt acaaatcagt agccaggccg ttggtaccag
     5041 cgccacccag gcgtaacgtt gacgtttcat cttgaacaac accacggcac agagcatcag
     5101 cgccatccct gccagcatct ggttggcaat accaaacagc ggccacagag tgttaatacc
     5161 gcccagcgga tcgaccacgc cctgatggag gaagtagccc cacgccagta cgcacagcgc
     5221 cgttgccagc aggttagcag gcagtgaatc ggtccgcttc aggccaggag acaccacgcc
     5281 cagcagatcc tgcaacataa agcgcgcagc acgcgtaccc gcatctaccg ccgtcagaat
     5341 aaacagagct tcaaacagaa tggcgaagtg ataccagaac gccacatcca tcataccacc
     5401 cagcgcaccg tgcaggatgt aagccatccc caccgccaac gtcggcgcac cgcccgcacg
     5461 ggaaatgatc gactgttcac ccacttcgct ggcaatctgg ttgagcgtat ctggtgtaat
     5521 gctaaagccc cagctactca ccacctgcgc ggcagaagcg accacatccg ccgtccctgc
     5581 cggagccagc accgccattg ggctgttcat ggcaaagtaa acgcccggat cgatgataca
     5641 ggcggaaacc agcgccataa tcgccacgaa ggattccatc aacattccac cgtagccgat
     5701 aaaacaagcc tgcccttcgt tcgccagcat cttcggcgtg gtgccggaag agatcagcgc
     5761 atggaagcca gacaccgcgc cacaggcgat ggtgataaac aggaacggga acaggttacc
     5821 ggtccatacc gggccagtgc catcgacaaa tttggtcagc gcaggcatgg tcagcgtcgg
     5881 gcgcataatc aaaatgccta ccgccagacc gacgatcgtc ccgattttca ggaaggtaga
     5941 gaggtagtca cgcggggcca gcagcaacca caccggcagc accgccgcca caaaaccgta
     6001 acccaccagc atccaggtca gctgcacgcc ggtaaagtca aagtacggtg cccaggtcgg
     6061 actttctgcc acccagccgc cagagataat ggcgaaaatc aggaatacca gaccgatgac
     6121 cgacacttca ccaatacgcc ccggacgcag atagcgcagg tagatcccca taaacagcgc
     6181 cagcggaatg gtgaacgcaa cggtatacgt tccccacggg ctatgagtca gggctttcac
     6241 cacgatcatc gccagtactg caaggataat gaccatgatc ataaagcagg ccaccagcgc
     6301 tatcaccccg gcggttggcc ccatctcttc tttgaccagc tcacccagcg atcgaccgtc
     6361 acggcgcgta gaaacaaaca gcaccatgaa atcctgcacc gcaccggcga gaaccacccc
     6421 ggcgagcagc cagatcatcc ctggcaggta acccatttgc gccgccagta ccggcccaac
     6481 caacggacct gctccggcaa tggccgcaaa atggtgaccg aacagcactt tcttgtccgt
     6541 cggcacatag tccagcccgt cgttatggcg caccgctggc gtcatacgcg tcgggtcaac
     6601 cgccagcaca tttttggcga tatacagccc ataaaaacgg taagcgatca gatagataca
     6661 gaccgacgcc accacaatcc acagcgcgtt gatctgttcc ccacgattta aagcaatgta
     6721 tcccagagca aatgctccca ttacagagag cactgtccag acgaggtatt tccctgattt
     6781 gttcatagtt gttatccgtg tgcgtgtcca tagagatgtt acattttgtt tctataacat
     6841 ctttgtacga tttaacaaca cggtaaccat gtaaatcgga gttgaaacca atatttaact
     6901 taacaatgtt aactcgatca ctccgttaac cgagttttcc tgcaatctca tttaattctg
     6961 tcgggctcat cccaaaactg ccgtacccag ccgacaagtg cagcaacgcc gcccctgttc
     7021 atcgaaaacg acgatttccc agctctgatt ttgccgccca agatgcagcg gctggcagac
     7081 gccgcgtacc ttgccttcag acaccgggcg atgatgggtg gcgttaagtt ccgtacccac
     7141 cacacactgc ccgtcacggg tcatcataaa tccggccatc gatcccagcg tttcagccag
     7201 cgccgccgac gcgccgccat gcagcaggcc aaacggctga tgagtacggg tatcaaccgg
     7261 catttcggct tccagcacat catctcccag acgggtatac acaattccca gatgcgccac
     7321 cattgtgtta tcgctggtgg cgttcagttc gtcgagcgtt aaatggcgtt tccagatcat
     7381 gcttatgccc ccagcgttga gccgccatcg accacaatat cctgtagggt gatatggctg
     7441 gcgaggtcag aggcgaggaa caaaatcgtg ttggcgatct cttgtggacg ggcgattttc
     7501 cccagcggaa tgccgagttt aaactgctcg ccaaagccgc gaatacgctg ttcttcggcg
     7561 tcatcgctca cccacagcgt gcgttgcata tcggtgtcgg tggagccagg cgaaaccaca
     7621 ttacagcgca cgccgctacc cgccagttcc agcccgacgc tcaacgccag gcttttcagc
     7681 gccgctttcg atgcgccata agcactcata ccaatacgcg gcgtgtgtgc ggcgtcggac
     7741 gccacagtga caatcgcccc gccccgctga cggcgaaact ggttcatggt ttgctggaac
     7801 aggttaaacg caccgccgac gttaaccgca aaagtctgct gccagtcctc tttactgagc
     7861 tgatcggtcg cgcccatgcg caaaattccc gccgcattga tcagcacgtc cagtcgctcc
     7921 gtttcagcta acagtcgctg acatacctgc gccacctgcc cggcgtcggc aacatccatt
     7981 acttcggtcg caaagggata ttgctcctga gtgaacgctt gatcaaaacc tgtaactttc
     8041 gctcccgctt caacaaacgc cagcgccgtg gcgtagccga tacctttacc tgcgccggtt
     8101 acccagacat ttttaccgct gaaatccatt atttcacctc gcgggagagt agcttccacc
     8161 aggcgtcgat ggtcgggttt ttcgccagca tgacaaagtc gatatcgcca tgcactttgc
     8221 gccagcgcgc cgccagcgcc atcatgcgca ccgaatccag accgtagtcg atcaggttgt
     8281 cgtcatcgaa cggttcatcg gactcgtcca gcaacggcag gataacctca cgcagcgccg
     8341 ctttgctggc ggggacaggt gctggcagta attcttcagt catcaccacc cggccagacc
     8401 gcccggccac atatttcagc gacatcaaat gctcgtcacg gctgaaatcg gccagcgcat
     8461 ccgccaccat aaacggttta atatcgcgca taaatgcgtc ggttgcggta gtcatacagc
     8521 caatgtgggc atataccccg gtaataatca gctggttacg tccactctct ttgagcattt
     8581 gctccagcgg agaacgatga aacgcgctgt agcgccactt caccagcacc gtgtcgtcgg
     8641 catctggcgt caggcgatcc accacctttt gctgttccgg cgaacgggtc aggcccggcc
     8701 cccacatatc attcaacagc gcccgatctt catcgctctg ctctttcggc tgggcggtgt
     8761 aataaaccgg aatattgtgc tgtttgcagt agtcgcgcag cgcagcaata ttcgcgatca
     8821 cctgctccat catcgggcag ttctcgcccc agaagctgac aaaatagtcc tgcatatcat
     8881 ggattaacaa cgcggcacgt tgcggttcaa aggcccagtc aactttattc ttagggatat
     8941 cgtgagactc cggcagtgcg taagcctgta attttggaat agccatcgtg ttctctcctt
     9001 caggctgatg cgcgtgacgc cagccactga cgtaattgtt ttttatcgac tttcccgacc
     9061 gccgtcagcg gaagtgaatc tacacactcc acgcgatccg gtaatttaaa ttcggcaata
     9121 ccctgttcac gcaggaaacg acgcacctgc accgcgcgca gcggctcttt taccaccaga
     9181 taagcgcagc ttttttcgcc catcagctca tcttccatgc tcaccagtgc ggcgtagatc
     9241 accgccgggt ggcgcagcag caggttttcg atctcttcgg cagcgatctt ctcaccgcca
     9301 cggttgatct gatctttctc gcgcccctgc acggtgatgt acccctctgg atcaatagag
     9361 atcagatcgc cggaacagta aaaaccgttg gcatcaaagg cgctggcatt gtgctgcggg
     9421 cttttgtaat agccacggaa ggtgtatgga ccgcgggtca tcaggcgtcc aacttcccct
     9481 tgcggcagtg gatttccttc agcatcagca acccatactt catcgtccgg acacattggg
     9541 taaccctggg tatggataat tttctccgcg ctatcatcaa gacgggtgta gttcaccagc
     9601 ccttccgcca tgccaaacac ctgctgcaac tggcagccaa tctcagcggg aatacgcgcc
     9661 gcaagggtgg cagaaagacg tgcgccgccg acctgtaaca gtttcagcga ggcaagctgc
     9721 gcccggcttt caccttcggt cagcgcctgc aaccacaggc tgactgccgg tggcaccagc
     9781 gcggtgacgt tcacctgatg tttttcaatc aatgggaagc aaagcgtggc gctgggatcg
     9841 gccgccagaa caaccgttcc tccggcaaga aagacgccca gcgatcccgg cgaactcatg
     9901 gcgtagttat gagccgccgg gatcgcgcac aggtagcgtg tctgtcgtgt gaactgacaa
     9961 atctcgacgc tacgacgcac gctgtagtag tagtcgttat gagtgcgcgg gatcagtttc
    10021 ggtgtgccgg tggtgccgcc ggaaagctgg aaataggcca cttcatcagc aggtgatggc
    10081 gtggcagtaa aatcctcagc cggatggtta atcgcatcct gcaagttatg ctcaccgctg
    10141 tcgttgtgca gttgcaccac gcgaatggag gaatgttctg tgacgaaagt attgaggaaa
    10201 tcatccccgc taaacagcgc atgttggcga tcggcaatca gcaatgcggg ttcaatctgg
    10261 ctggcatagg cgttcagttc actacgctga tggctgaaca gcgccagcac cggcgcaacg
    10321 cccagtttca gcagcgcgaa aaaggtaata tacaattcag cgacgttacc cagttgtacc
    10381 agcgcggttt caccaggttt aatgccctga cggcgtaaac tacacgcgag gttatccgcc
    10441 gcctgattca gctcccggta actcaactgt cgctcgccgt cgataaccgc gatgctgtca
    10501 ctcgcagcgt ggcgagtcag aatgtcggtc agcggcaaat cctgccagta gcctttttcc
    10561 cgatagcgac gggcaaactc ttccggccag cgggtgaatg gaatgctcat cctcgctcct
    10621 taatgcaatc caaaaacgtt caacatggta gaaagtttga cgcctgtttc gcgccactca
    10681 cccaacggtg acgacgcagg cacaatcccc gctccggcaa acagacgcac ctgattttcc
    10741 cgcagcttcg cgcagcggat ggtcaccacc cattcgccgt taccttcgct gtcacaccaa
    10801 cccacaatgc cgccaaacag ttcgcggtcg aatggctcca gttcagcaat aacctgggtc
    10861 gcggcctgat gcgggaaacc gctcagcgcg ggggttggat gcagcagaca ggccagagtc
    10921 agtgcgtttt cttgcgaatt cgctttacct tcaaagggag ttgcgagatg ccacagcgtc
    10981 ggcgtggtaa tcaattgtgg agaggaggga acgtgtaact cactactgcg ttcgcgcagt
    11041 acctctttca tcgcctgagt caccagttca tgttcatggc gatctttttc tgacgccagc
    11101 agacgattac ccgcttcgcg atcgagcact tcatccggct gacgacgcgc ggaaccggct
    11161 aacggaatgg agctaaaacg ctcgccgtct ttacgtagca gcagttccgg gctggccccc
    11221 agcaggacgc cgccatcagc cagcgggaca tggaagttgt aactaaccgg gttttgcgca
    11281 atcaaccgtt ccagcaatac gccactatca atggcggcgt cagtggtgat atcaatcaac
    11341 cgtgacaaca ccactttgtc gacctgcggc gtggcggtaa gtgcggcagc gcgagcaacc
    11401 atctgttcaa acgtggtttg ttgcggaatt gcctggcgtt ctaccacgtt cagcgactgg
    11461 ctgcgggtga aacggcgggc ggaagcttgt ttttcctgac gggagaacga ctgccaggat
    11521 tcgggaatat acagcgacga aggctgacgt ggatcgaagg gaatcgcccc gaccatcacc
    11581 ggatttttga tgccctgcgc tttggcatcg gcaaacagcg cggcgagttt ttgctggaag
    11641 ggactgtcgg gcgaatcccc gttcacagcc ggttcatcga aacgggcgaa acatcctgac
    11701 gtcgtaaaac tgcggtacgg cgacataaag aaaaagcgat tgggcgcaag tgttgccatg
    11761 gtctgctgta cttcctcagc cagtgacgta tccatatcat cctccacaaa atgataaagg
    11821 ctttaataat gattatcatt tatattttcg gtcgctaacc taaaagcaaa cgcgcactat
    11881 gtcaactctt gaggtaacgc aatctgcaac tcggggttgc atctggttgt ggttacaatg
    11941 aaaatgagaa gcattaactt cattaataac aggaagctga tttgtgagac tcgccccgct
    12001 ctaccgcaac gcccttctat taacaggact tttactttca ggaatagccg cagttcaggc
    12061 cgctgactgg ccgcgtcaga ttactgacag ccgtggcaca catacactgg aaagccagcc
    12121 gcagcgtatt gtttccacca gcgtcaccct gaccggctca ctgctggcga ttgatgctcc
    12181 ggtgatcgcc agcggcgcga ccacgccgaa taaccgcgtc gcggatgacc agggcttttt
    12241 acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa cggctctata tcggcgaacc
    12301 gagcgccgaa gccgttgccg cgcaaatgcc ggatctgatt ttaattagcg caaccggcgg
    12361 ggattcggcg ctggcactgt atgatcagct ttccaccatc gccccgacat taatcatcaa
    12421 ttacgacgac aaaagctggc agtcgctgtt aacgcagctt ggcgaaatta ccgggcatga
    12481 gaaacaagcg gcagagcgga ttgcacagtt tgataagcaa ctggcagcgg cgaaagagca
    12541 aatcaaatta ccgccgcagc cggtcactgc cattgtctat accgccgctg cacacagtgc
    12601 caatctctgg acgccggaat cagcacaagg gcagatgctg gaacaactcg gctttacgct
    12661 ggcgaagttg cccgcagggt taaacgccag ccaaagccag ggcaaacgcc atgacatcat
    12721 tcagcttggt ggggaaaatc tggctgctgg gttaaatggc gagtcactat tcctgttcgc
    12781 cggtgatcag aaagatgccg atgcgattta tgccaatcca ctgctcgcgc acctgcccgc
    12841 agtacaaaac aagcaggttt atgcgctggg aaccgagacg ttccgcctgg attactacag
    12901 cgccatgcaa gtgctggaac gacttaaggc gctgttttaa gcattaactg tcagacgctg
    12961 tcacctgcgg cggcgtctgg cgaaaacgtc gcaactccac cagcaccagc aataacaaca
    13021 cgccgataat caacaaacca aaaccgctcg cgcttgcgga agcaaccggt gtcatcatcg
    13081 cgcccaaacc acccagcagc gccgcgccta tggcatcgcc cgtcacgttc tgcgccgtcc
    13141 acaaaccgtt aatccgccct aacatcgctt ccggggtttg cgtttgcagc attgtatatt
    13201 gcagcaacga actaaccgcg ctcagccagc cgaacagcgc cagacaaacc acgcctaaaa
    13261 tccacatcgg catcaggcca aacagaccaa tggcgaggaa cgatcccagc gtggagagca
    13321 gcatcaataa ccctggtcgc gcactatgtg ccagcttccc gctggttaac gcaccaatag
    13381 ccgcgccgag cgggatcgcc gcgtagagaa aaccaatctg tgccgctgac atctgccagt
    13441 tgtcagccag cgccggatac agtacccgca ccgcgctcgc catcgtcaat aaaccaccca
    13501 gcagcgcaat cccgcccacc agcgggctgg caagcagaaa acgaaatcct gccagtaatg
    13561 atttcaacgg atgctcacgc ggctgcggtg gcggtggcaa cgccggaagg cttaacaacg
    13621 gtagcaaggt aataaacgtg cccgccgccg ccagcccgta gttccaggct acgccaccgg
    13681 tcgccagcaa taaaccgcca atcatgggcg aaatcaccga ccccagacgc acggtcaaca
    13741 tggtgatcgc cccggcctgc attaagtttt cacgccctac cagtgctggt gtcgccgcca
    13801 atagcgccgt aacgccaagc gatgcgaaaa aaccatccca taaaccaagt aaatagattg
    13861 ccagcaatga cggctccggc agcagtgcat taaggcacag tccaatgaag ccaatgccac
    13921 aggtgccgcg cgccagcaaa atcacttttt tgcgctcata gcgatccgcc agcacaccgc
    13981 caaccatcag gccaacaaac atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc
    14041 aggtagaatg cgtcatcatc tggatctgca ccggcaccgc gacgccgagc aaacccagag
    14101 acacaattga gataaaacga gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca
    14161 ggctgaggtt aagcagccag gattgtttat tcattacaat gccttgccat caaatatttt
    14221 aaatacctat tcttaggctg cacatgctaa catatccaaa taagatcgat aacgataatt
    14281 aatttcatta tcatggaagt tcgtatgtct ggttctgttg ccgtgacacg cgccattgcc
    14341 gtgcccggat tgctgttatt actgattatc gcgacggcat taagcctgct cattggggca
    14401 aaatcactcc ccgcttccgt agtgctggag gccctctccg gcacctgcca gagcgccgac
    14461 tgcaccatcg tgctcgacgc gcgactgccg cgcacccttg ccggtttact tgcaggcggc
    14521 gcgcttggcc ttgccggggc gttgatgcaa accctcaccc gaaatccact tgccgacccc
    14581 ggcttgcttg gcgtgaatgc cggagccagc tttgccattg tgctgggtgc ggcgctgttt
    14641 ggttactcat ccgcgcagga acaactggcg atggccttcg ccggggcgct ggtggcctca
    14701 ttgattgttg cctttaccgg cagtcagggc ggcgggcagt taagtccggt gcgtttaacc
    14761 ctggcgggcg tggcgctggc ggcggtgctg gaagggctga ccagcggcat cgccctgctt
    14821 aatcctgatg tctacgatca actgcgtttc tggcaagccg gttcgctgga tattcgcaat
    14881 ctacatacct taaaagtggt gctgatcccg gtgctgatcg ccggagcaac cgcgctatta
    14941 ctgagccgcg cgctgaacag tttaagcctc ggcagcgaca ccgcgacggc gctgggcagt
    15001 cgcgtggcgc gcacacagtt aattggtctg ctggcgatta ccgtgctttg tggtagtgcg
    15061 acggcggtag ttggcccgat tgcctttatc ggcctgatga tgccgcacat ggcgcgctgg
    15121 ctggtaggtg ccgatcatcg ctggtcgctg cccgtcacgc tacttgctac ccctgccctg
    15181 ctgctgtttg ccgatatcat cgggcgggtg attgtgcccg gcgaactgcg cgtttctgtg
    15241 gtcagtgcgt ttattggtgc accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt
    15301 ggcgcatgat ttacgtctct cgccgattac tcatcacctg tttgctgctt atttccgcct
    15361 gtgtggttgc aggtatctgg ggattacgca gcggtgccgt cacgctggaa acctcgcagg
    15421 tattcgccgc gctgatgggc gatgcgccgc gcagtatgac gatggtggtc accgaatggc
    15481 gtttaccacg cgtgctgatg gcgctgttga ttggcgcggc actgggcgtc agcggtgcga
    15541 tttttcagtc gctgatgcgt aacccgctcg gcagccctga cgtaatgggc tttaacaccg
    15601 gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg tcaggacctg acggctatcg
    15661 cgctggcagc aatggtgggc ggcattgtca cttctctgtt ggtctggctg ctcgcctggc
    15721 gtaacggtat cgacaccttt cggttgatta ttatcggcat cggcgttcgc gccatgctgg
    15781 tggcctttaa tacctggctg ttgctgaaag cgtctttaga aacggcgcta acagcgggtt
    15841 tgtggaatgc cggatcgctc aatggcctga cgtgggcaaa aacttcgcct tccgcaccca
    15901 tcattatatt gatgctcatt gccgccgcct tgctggtgcg gcgaatgcgc ttgctggaga
    15961 tgggcgatga taccgcctgc gcgctgggcg tcagcgtcga gcgttcgcgt ctgttaatga
    16021 tgctggttgc agtggtgctt accgctgcgg caacggcgct tgccgggccg atttccttta
    16081 ttgctttagt cgcgccgcac attgcccgac gcattagcgg caccgctcgc tgggggctga
    16141 cccaggcggc gctgtgcgga gcgctgttac tgctggcggc cgatctctgc gcccaacaac
    16201 tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt cagcctcggc ggtatttacc
    16261 ttatcgtctt gttaattcag gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg
    16321 cggcgaacag ttaaccctgg gatatggcaa atataccgtt gcggaaaatc tgactgtaga
    16381 aattcctgat ggtcacttca cggcaattat cggaccaaat ggctgtggta aatccacgtt
    16441 actgcgtacc ttaagccgcc tgatgacgcc tgctcacggg catgtctggc tggatggcga
    16501 gcacattcaa cattacgcca gtaaagaagt tgcacgccgg attggtctgt tggcgcaaaa
    16561 tgctaccacg ccaggtgata tcaccgtgca ggagctggtg gcgcgtgggc gttacccgca
    16621 tcaaccgcta tttacccgct ggcgcaaaga agatgaagaa gcggtaacga aagcgatgca
    16681 ggccacggga ataactcatc tggcagatca aagcgtggat accctttctg gcggacaacg
    16741 ccagcgcgcg tggatcgcga tggtgctggc ccaggaaacg gcaattatgc tgctcgacga
    16801 acccacgacc tggctggata tcagtcatca gattgatcta ctggaactgt taagcgaact
    16861 gaaccgcgag aaaggctata ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg
    16921 ttacgccagc catttgattg cattgcggga agggaaaatt gttgctcagg gagcgccgaa
    16981 ggagattgtc actgctgaac tgattgagcg catttatggt ctgcgatgca tgatcattga
    17041 cgatccggta gccggaacgc cgcttgtggt gccgctcgga cgaacggcac cttcaaccgc
    17101 aaatagttaa actaagtggt ctgccatcat ggcatcctgt tttctggatg ccatcgcata
    17161 gcgcaataac acgctaccac aagccaccat cccgccgatc agcgcggaaa ggatcacaat
    17221 aatcgcctta cccggaccgt cttttttcac tggcaatgac gggcttaact gatatttaaa
    17281 cggcgtaaaa ttcacatcgt tgatatttgc ttttgttaac tgctcaacaa gatactgacg
    17341 attacgtaat tcaccgttca gttccgcaac gtcagtgacc gctttttcaa tttccagttt
    17401 gcgttcaata ccgtctgcgc cgagagaaat agagaaatcg gggtcatctt taacggcctg
    17461 accattactg tatacgggct ttttaattcc cgccgcgttg gcaatgtcga gtgaataatt
    17521 gaggcgctga atgtttgcat caagttgatt tttcgtttta atgcgatcct gagccagttt
    17581 ttctttttca aactgggttt tgatttccag tttattacgg acgttttcta gtgactcttt
    17641 caccaccaac gcagagatat aatcgatata cccgctcaaa acggtctgcg cctcttcact
    17701 ggttggcgcg gtaaaactta gcgtccagga ggtatacagt gacggttcat cttttttctt
    17761 actggcattg tcatcaaccg ctttcatttt ttcgctcaat gcaacaatag cgcgatgcaa
    17821 atccagttca tcgattttcg cctcttttaa ttggtccatc acataaggtg atgaacgcag
    17881 gtactcttcc agcaagctaa ccgactgaaa cttcttgata aacaggttaa atgcttctgt
    17941 acgatcaatt ttgatatcca gatccagcac tcgaagctta gtgaatgttt tctccagctc
    18001 ttgccactga acaggttctg gaggcgtgac aaccgccgcg ctggtccatt tttgcggcag
    18061 gatgaaagag atcagcaagc ctgcgcaggc aaacgcaaaa atgaccgcca tgaccgtttt
    18121 tttggcccgc cataaaactg cgattagatt aagtaaatca atttcattat tactgggcga
    18181 cgccagagga tactcaggaa aatgagcgtc acttccctgt ttaatattca gtgatgacat
    18241 ggggtataac ctgaaaatga gtccaatagg ggaaatctcc acggcgctca ctttaccagt
    18301 tggtgataat tatccgaagc tgaagtttgt aaattccttc
//
LOCUS       DAPHDX010000086        18302 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPHDX010000086 DAPHDX010000000
VERSION     DAPHDX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.086"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..583
                     /locus_tag="RH058_002991"
                     /pseudo
     CDS             1..583
                     /locus_tag="RH058_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            686..1189
                     /locus_tag="RH058_002992"
     CDS             686..1189
                     /locus_tag="RH058_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV3138549.1"
                     /translation="MGMIGYFAEIDSEKINQLLGSTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1256..2713)
                     /gene="nuoN"
                     /locus_tag="RH058_002993"
     CDS             complement(1256..2713)
                     /gene="nuoN"
                     /locus_tag="RH058_002993"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV3138550.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2720..4249)
                     /gene="nuoM"
                     /locus_tag="RH058_002994"
     CDS             complement(2720..4249)
                     /gene="nuoM"
                     /locus_tag="RH058_002994"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV3138551.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4413..6254)
                     /gene="nuoL"
                     /locus_tag="RH058_002995"
     CDS             complement(4413..6254)
                     /gene="nuoL"
                     /locus_tag="RH058_002995"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV3138552.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6251..6553)
                     /gene="nuoK"
                     /locus_tag="RH058_002996"
     CDS             complement(6251..6553)
                     /gene="nuoK"
                     /locus_tag="RH058_002996"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV3138553.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6550..7104)
                     /gene="nuoJ"
                     /locus_tag="RH058_002997"
     CDS             complement(6550..7104)
                     /gene="nuoJ"
                     /locus_tag="RH058_002997"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV3138554.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7116..7658)
                     /gene="nuoI"
                     /locus_tag="RH058_002998"
     CDS             complement(7116..7658)
                     /gene="nuoI"
                     /locus_tag="RH058_002998"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV3138555.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7673..8650)
                     /gene="nuoH"
                     /locus_tag="RH058_002999"
     CDS             complement(7673..8650)
                     /gene="nuoH"
                     /locus_tag="RH058_002999"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV3138556.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8647..11373)
                     /gene="nuoG"
                     /locus_tag="RH058_003000"
     CDS             complement(8647..11373)
                     /gene="nuoG"
                     /locus_tag="RH058_003000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV3138557.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11426..12763)
                     /gene="nuoF"
                     /locus_tag="RH058_003001"
     CDS             complement(11426..12763)
                     /gene="nuoF"
                     /locus_tag="RH058_003001"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV3138558.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12760..13260)
                     /gene="nuoE"
                     /locus_tag="RH058_003002"
     CDS             complement(12760..13260)
                     /gene="nuoE"
                     /locus_tag="RH058_003002"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV3138559.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13263..15065)
                     /gene="nuoC"
                     /locus_tag="RH058_003003"
     CDS             complement(13263..15065)
                     /gene="nuoC"
                     /locus_tag="RH058_003003"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV3138560.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15159..15821)
                     /gene="nuoB"
                     /locus_tag="RH058_003004"
     CDS             complement(15159..15821)
                     /gene="nuoB"
                     /locus_tag="RH058_003004"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV3138561.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15837..16280)
                     /gene="nuoA"
                     /locus_tag="RH058_003005"
     CDS             complement(15837..16280)
                     /gene="nuoA"
                     /locus_tag="RH058_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV3138562.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16911..17849)
                     /gene="lrhA"
                     /locus_tag="RH058_003006"
     CDS             complement(16911..17849)
                     /gene="lrhA"
                     /locus_tag="RH058_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV3138563.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYILQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4570 a         5085 c         4722 g         3925 t
ORIGIN      
        1 gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca cgtcagtcct
       61 gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg taatgccgac
      121 aatattgata atctgaatag gtccgtagag tatataaaag agttctttgt cagtagcggt
      181 gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc ctggtagcca
      241 ggattaccct tacccgcaat gtcctggctt tatcccgatc ggggggactt tattgcctgg
      301 tcggcagaat acaggatatc aacgccgttc gtcaggtaaa aacggcattg ttatcatccc
      361 gggatttatc tgtttattct atgaatgccc catggtttat tcccggtatt gatttctctg
      421 accacctgaa ttattggcaa cacgatattc ccgccgtaat gattactgac accgcttttt
      481 atcgtaataa acaataccac ttgcccggtg atacagcaga cagattgaat tatcagaaaa
      541 tggctcagat ggtgttataa ccttgttaca caacagtaaa taataaaaat ttctcgaggc
      601 gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa gatgcgaaac
      661 ataaaattca atggaggaaa aataaatggg aatgattggt tattttgcag aaattgactc
      721 agaaaagatt aaccaactgc ttgggtctac tgaaaaacct ttgatggata atatacatga
      781 cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg
      841 gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc gtgccgtgct
      901 tggtgaacac agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga
      961 gttagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc gtaaacagtt
     1021 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
     1081 agaggggcaa ctctttgctt cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
     1141 acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcaggg
     1201 caccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaactg atagcttaca
     1261 tcagcggcat tgctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca
     1321 gtaccaacag tgcagagatc agcaccacga taccgcctgc gctgtactgc cagtttgacg
     1381 gtgcatcgcg acccggttgt tccggggcgt gcaggtacag gctcaccgcc acgcgcaggt
     1441 agtagtaaag gccgattgcc gaaccgacaa ccacggcgcc caccagccac cacaagtgtg
     1501 cctggacacc gaccgccagc acgtagaact taccaataaa gcccagcgtc atcggaatac
     1561 cggccagaga cagcatcatc accgtcatca ccgccgcgag gatcgggcga tgccagaaca
     1621 gaccgcggta agagaacagg gaatcagcat ccgggccacg gtacgggctg gacatcaggc
     1681 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa actcctaccg
     1741 cttccatcga catctcgccc gtttgcagcg caatcagcgc caccagcaga tagccgaggt
     1801 gagagataga tgagtaaccg agcagacgtt tgatattggt ctggctcagc gccatcaggt
     1861 taccgaagat gatagaggca aaggcgataa tcgccagcac tacccgaatc gcttcgctgt
     1921 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     1981 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     2041 ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcagt ccgaaacctg
     2101 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg
     2161 acaggtcgcc agactgtgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg
     2221 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
     2281 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac aaagacgcca
     2341 gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     2401 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     2461 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     2521 gcgtaacgtc catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa
     2581 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     2641 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     2701 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     2761 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     2821 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     2881 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca
     2941 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
     3001 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     3061 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     3121 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa
     3181 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
     3241 ccaactggct gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt
     3301 aggcgatcag acgtttgata tcggtctggg cgaaggccat ccacgcaccg tagaagatgc
     3361 cgataacacc cagccacata gcaattggcg cgaactctgc cgacgcgttc gggaacagcg
     3421 gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa
     3481 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
     3541 ccggcatttt gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac
     3601 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     3661 agtgaacaaa aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt
     3721 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     3781 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     3841 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     3901 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca gagtaccgcc agcacaccga
     3961 gcagaccggt cagcacgacc atcagcagcg acagaccgtc aatggcgaga tgaatagaga
     4021 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
     4081 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
     4141 ccatggtgat cagcgcgatc cagcgcggca ccttgacgcc aaagcgttcg gtctgccagc
     4201 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
     4261 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
     4321 cttcggactg ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt
     4381 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
     4441 accacgaccg caccgatgct catggatgcc acataccagc gcagatagcc gttctcactt
     4501 aacagcagac ctttacctgc aaagcgggaa aggacagccg ggatgttcat cattgagttc
     4561 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtca
     4621 tacagccagt caaatcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca
     4681 ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga
     4741 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
     4801 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
     4861 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
     4921 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
     4981 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     5041 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggtag tgccgacagt
     5101 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     5161 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     5221 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     5281 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     5341 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     5401 atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc
     5461 aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg
     5521 cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     5581 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     5641 ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac
     5701 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgccgct
     5761 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     5821 ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg
     5881 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     5941 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc
     6001 gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
     6061 cacagcggct ggctgtatga ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     6121 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     6181 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     6241 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     6301 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     6361 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     6421 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     6481 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     6541 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     6601 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     6661 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     6721 tgctttagca ctgattggcg taccgtcgat accctgatca ttaacaccaa ggatggcgta
     6781 aacaatcacc accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt
     6841 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
     6901 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     6961 agcgcccagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
     7021 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     7081 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     7141 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     7201 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttt
     7261 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     7321 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg
     7381 cggaaaaatt ccgggtacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     7441 ccgaccgggc aggctaccgc gcagaggtta caggctacgc aacgctcttc gccgtccggg
     7501 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     7561 tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc
     7621 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     7681 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     7741 ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca
     7801 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     7861 acgggccttg ccagccaccg aagaacagcg tcaccatcag tgcagagatg gtcacaatcc
     7921 cgatgtactc acccacgaag aacaaaccga acttcatacc ggaatattca atgtggtaac
     7981 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca
     8041 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc cacacatgcg
     8101 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     8161 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     8221 gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     8281 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccgggc
     8341 tgacaggcac aatcgcaaag gccagcagca gtgaggtaaa ggcaatcatc ggtgccaggg
     8401 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     8461 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct
     8521 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag gtgacaacca
     8581 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     8641 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     8701 aatgccggac atacccatcg gcaaacccac ctgccctgcc gtcagccctt cggcgatttc
     8761 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgtgtgtac ctgcgttcac
     8821 acctaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     8881 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat
     8941 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     9001 attttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     9061 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     9121 cacttgcgaa cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     9181 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     9241 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     9301 cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac
     9361 atggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa
     9421 ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg
     9481 gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct
     9541 ggcagcagaa agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac
     9601 catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg
     9661 cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc
     9721 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
     9781 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
     9841 ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
     9901 tgccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac
     9961 cgctggcgca gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
    10021 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt
    10081 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
    10141 agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc
    10201 cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
    10261 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
    10321 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
    10381 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
    10441 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
    10501 aatgaaatca tcgccacgac gctgtactgg ttgacgcgga cgatctttca ggttgacgta
    10561 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
    10621 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
    10681 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    10741 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    10801 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac
    10861 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat
    10921 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
    10981 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcaca gtcatatcct gaagatggca
    11041 gttaccgccc tcttcacata ccggacagtc gtgcgggtgg ttggtcatca accactcgac
    11101 cacgctttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    11161 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    11221 cttcaccgca cactggcggc aagcaccgac acttcccagc gccggatgcc agcaaaagta
    11281 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    11341 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcagtcag
    11401 agactgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    11461 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    11521 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    11581 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctggcctt
    11641 cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    11701 tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca
    11761 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg cccgctttac
    11821 cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag tcggtgcccg
    11881 cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg gcgtaatctt
    11941 cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga tttttcaccc
    12001 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    12061 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
    12121 aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag
    12181 cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
    12241 ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca
    12301 agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt
    12361 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
    12421 cttccaccag caggtgcggc agttgctcca tcagcaggcg gtctttatag gtgcctggct
    12481 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    12541 ggctccattt caggccggta gaaaagcccg cgccaccgcg ccctttcaga ccagcgtctt
    12601 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gcgccttcgt
    12661 aaccgttttt gctgcgatat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    12721 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    12781 ttcagggatc gcttccgggg tcagatgcgc gtgagtatcc tcatcgatca tcatgtttgg
    12841 ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt
    12901 cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac cctgataccc
    12961 gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggctggcg
    13021 gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac
    13081 atcggcgatc gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt
    13141 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    13201 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    13261 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    13321 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    13381 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
    13441 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    13501 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    13561 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    13621 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    13681 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    13741 cgcgccttac gcacgtcgaa atcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
    13801 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    13861 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    13921 tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
    13981 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    14041 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg
    14101 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    14161 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    14221 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    14281 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    14341 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    14401 ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct
    14461 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    14521 ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    14581 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    14641 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    14701 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    14761 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    14821 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    14881 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    14941 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat caccggatca
    15001 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    15061 accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    15121 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    15181 aggttggtta cggcaatacg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    15241 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    15301 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    15361 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    15421 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    15481 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    15541 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    15601 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    15661 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    15721 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    15781 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    15841 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    15901 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    15961 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    16021 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    16081 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    16141 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    16201 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    16261 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    16321 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg
    16381 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    16441 caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa
    16501 tgccctttca ccccagatcc ttgaccactc caggataatt agatgttgtt gaatcgtgtc
    16561 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    16621 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac
    16681 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    16741 tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc
    16801 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    16861 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcaata
    16921 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    16981 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    17041 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    17101 agcacacgca ggtccgggct catcatttca accggccttg ccgtcacgcc aagaccggct
    17161 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    17221 tctgctttat taagcgtcgc cagcaccata tcgcgaaacg ggctaggatc atccagcaat
    17281 acaagaggga tcggctcgcc tttttgcaat atgtattccg ctgcgcagta ccagtgtgtt
    17341 ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa
    17401 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    17461 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    17521 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    17581 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    17641 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    17701 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    17761 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    17821 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    17881 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    17941 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    18001 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    18061 ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg
    18121 tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc
    18181 aatggtgagg cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa
    18241 accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca
    18301 aa
//
LOCUS       DAPHDX010000087        18179 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPHDX010000087 DAPHDX010000000
VERSION     DAPHDX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.087"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(150..956)
                     /gene="trpA"
                     /locus_tag="RH058_003007"
     CDS             complement(150..956)
                     /gene="trpA"
                     /locus_tag="RH058_003007"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV3138564.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(956..2149)
                     /gene="trpB"
                     /locus_tag="RH058_003008"
     CDS             complement(956..2149)
                     /gene="trpB"
                     /locus_tag="RH058_003008"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV3138565.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="RH058_003009"
     CDS             complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="RH058_003009"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV3138566.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="RH058_003010"
     CDS             complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="RH058_003010"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV3138567.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="RH058_003011"
     CDS             complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="RH058_003011"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV3138568.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6702..6795)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="RH058_003012"
     CDS             complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="RH058_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV3138569.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6954..7835
                     /gene="yciV"
                     /locus_tag="RH058_003013"
     CDS             6954..7835
                     /gene="yciV"
                     /locus_tag="RH058_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV3138570.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7832..8452
                     /locus_tag="RH058_003014"
     CDS             7832..8452
                     /locus_tag="RH058_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV3138571.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8553..9425
                     /gene="rluB"
                     /locus_tag="RH058_003015"
     CDS             8553..9425
                     /gene="rluB"
                     /locus_tag="RH058_003015"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV3138572.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9465..10055)
                     /gene="cobO"
                     /locus_tag="RH058_003016"
     CDS             complement(9465..10055)
                     /gene="cobO"
                     /locus_tag="RH058_003016"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV3138573.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10052..10810)
                     /locus_tag="RH058_003017"
     CDS             complement(10052..10810)
                     /locus_tag="RH058_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV3138574.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11030..12079
                     /gene="sohB"
                     /locus_tag="RH058_003018"
     CDS             11030..12079
                     /gene="sohB"
                     /locus_tag="RH058_003018"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV3138575.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12115..12366)
                     /locus_tag="RH058_003019"
     CDS             complement(12115..12366)
                     /locus_tag="RH058_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV3138576.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12746..15343
                     /gene="topA"
                     /locus_tag="RH058_003020"
     CDS             12746..15343
                     /gene="topA"
                     /locus_tag="RH058_003020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV3138577.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNKYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15553..16527
                     /gene="cysB"
                     /locus_tag="RH058_003021"
     CDS             15553..16527
                     /gene="cysB"
                     /locus_tag="RH058_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV3138578.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            16822..16986
                     /locus_tag="RH058_003022"
     CDS             16822..16986
                     /locus_tag="RH058_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV3138579.1"
                     /translation="MLISDSDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            16989..17156
                     /locus_tag="RH058_003023"
     CDS             16989..17156
                     /locus_tag="RH058_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138580.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            17270..17365
                     /locus_tag="RH058_003024"
     CDS             17270..17365
                     /locus_tag="RH058_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138581.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            17529..>18179
                     /locus_tag="RH058_003025"
     CDS             17529..>18179
                     /locus_tag="RH058_003025"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDV3138582.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTM"
BASE COUNT         4460 a         4567 c         4782 g         4370 t
ORIGIN      
        1 atattactgt tgggcggaaa aatgacgtaa gttgacgttc gaccggggta agcgaaacgg
       61 taaaaagata aatattaaat gaatttagga tttttccggc ttcattaaag aaagttaaaa
      121 tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt
      181 acaaaaactt tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt
      241 ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc
      301 tgatccgggg cggaaatacc aaacccctgc aatggaggtg cagcgttgta ctctttcagc
      361 ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcccgct
      421 cgtgacagca aataggtgta accacgaccg taagaggcta tctggcgcag caggtcgtca
      481 tcggcatttg gcgggcagat gaagataggt gcgacattat ggcgcaacgc ggcctggcgg
      541 aagggcgcgg actcttcaat tggcacatcg gcaaccagca ccgaatcgac gccgactttc
      601 tcgcactggg cataaaactc atcaatgcct ttgttaaaca ccagattggc atacatcaac
      661 aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc aaaacattgt
      721 gccggagtca cacctgccgc aaaggcgcgc agagtggcgt tttgaatggt cgggccatcc
      781 gccagtgggt cggagaaggg gatacctaac tccagcgcgt cagcaccggc ttcaattagc
      841 gtatcgataa ttttcaatga ctgctcaatg cccggatcac cgagcgtgac gaaaggaacg
      901 aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga
      961 tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg
     1021 aaaggttaac caccagtagc tgctctttat ccgggttttc gcgcatcatt ttcaacgcat
     1081 gggccagggc gtgggaggat tccagcgccg ggatgatccc ttcgtgcagg cacagcgttt
     1141 tgaaggcttc aagggcttca tcatcggtaa tagacacgta atcagcgcgt ccagtgctgt
     1201 taagatacgc gtgttgtggg ccgacagacg ggaaatccag tccggcggag atggagtaag
     1261 attcttcaat ctgcccgtct tcggtttgca tcatcggcgc tttcataccg aaatagatac
     1321 ccacgcgacc atgttttagc ggtgcgccgt gctcgccagt ttcgataccg tgaccacctg
     1381 gctccacacc aatcaggccg acgttggttt cattgatgaa atcagcaaac atgccgatgg
     1441 cattcgaacc gccgccaaca caggcgataa cggcatccgg caggcgacct tctctttcca
     1501 gaatctgcgc tttggtttct tcgccaatca tccgctgaaa ctcacgcaca atggtcggat
     1561 aaggatgcgg gccagctgcg gtgcccagca tatagtgcgc ggtttcgtaa ctaccggacc
     1621 agtcgcgcag cgcctcgtta caggcatctt tcagcgtcgc ggaaccgcta tgcaccggga
     1681 tcacttccgc acccattaag cgcatacgaa aaacgttagg cgactggcgt tcaacgtctt
     1741 tggcacccat ataaatacgg catttcaggc cgagcagggc gctggcaagg gccgacgcca
     1801 cgccatgctg accggcaccg gtttcggcga tgatttcggt tttacccatc cgcttcgcca
     1861 gcaacgcctg ccccagcacc tggttagttt tatgcgcgcc gccgtgcagc aaatcttcac
     1921 gcttgagata cagcgtggtg ttcgtcccgg ctgtaatgtt ctggcatttg gtcagcgcgg
     1981 ttggacgccc ggcatagttt ttcagcaggt cgttgaactg agcctgaaat tcaggatctt
     2041 tttgcgcact gacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca
     2101 cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt gttcctttcc
     2161 ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatgcccgg
     2221 ttgcgactct acagcagaat taaaatcaag tccggcgcag ccggtttgtg ccgcttccac
     2281 gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccatttaa
     2341 tagtgaccag tcaaaacgtt gcccgcttcc accctggccg ttgtctaaaa catatttatc
     2401 aacgtgctga aactcgcggg cgggcagggt ttcaccgacg cttaatgctt tccagatggc
     2461 aacatgtgct ggcagagctt cacgcagcgt atcgatatac agctgttctt cattaccatg
     2521 cagttgcact gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg
     2581 attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc
     2641 aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc
     2701 gtcataagct gctttagcat cttgcccacg cgtcaggcca catactttat tctcacccag
     2761 caacacccgg cgcacggcgg cgtgcaaatc gtcatgggcc atcaacgccg aaccaatcag
     2821 aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc
     2881 gctgattacc gtcacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc
     2941 aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg acctttgccc ccaatgcaat
     3001 ggcacgctcc agttcctctt cattactgac ttcggtcagc acacccatct ccagactgtg
     3061 ggcgacggcg gcaagctggc gatattgttc gtcatccagt actgaaagca ttaataagca
     3121 ggcatcagcc tggtaatagc gcgccagata gatctggtaa ggatcgataa tgaagtcttt
     3181 acataaaatc ggctgcgggg cgatttggct gacgatgggg aggaaatcaa agctcccctg
     3241 aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg taatgtttat aaatggcggc
     3301 aatgcgtgct ggatcgaaat catcacggat cacgcctttt gacggcgacg ctttcttgca
     3361 ctccagaata aacgccgtgc gcgcaccctg tagcgcatca taaaaatgtc gcgtgctcgg
     3421 ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggttt ctacccaaat
     3481 cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc atttaccctc gtgccgccag
     3541 tgcggtaact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt
     3601 ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacgttcg cagcgacggc
     3661 tgcttcatgg gctgcgtcgc ctttaccttg taacaagcgt gttaaaatgt cacggttttc
     3721 ttccggtgtt ccgcctgcca gttgctcctg gtggtaggga gtcaggccaa aatcttcagc
     3781 ggtcaattga tagctcttaa tttcgccgtc atgcagctcg gcaacgattg tcggcgcgtg
     3841 taatgaaact tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat accccagcac
     3901 gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttaacgccag
     3961 cggcggatgc gccgggttaa tcaatggccc cagcacattg aacagggtgc gggttttcag
     4021 ttgctggcga accggcatcg cgtggcggaa tccggtgtga tacttcggcg caaagaggaa
     4081 acatacacct aactcatcca gcgcctggcg cgatttatcg gcgttcatat caagattaat
     4141 accgaacgcc gccagcagat cggacgaacc agatttactg gagacgctac ggttgccgtg
     4201 tttcgccact ttcagcccac aggccgcggc gacaaacgca ctggcggtag aaatattgat
     4261 actgttgctg ccgtcaccgc cagtaccgac gatatcagca aacagataat ccgggcgcgg
     4321 gaacggcgcg gcgttttcca gtagcgcggt tgctgctccg gcgatctcgt tcgggtgctc
     4381 accgcgaatt ttcatgctca ccagcgccgc cgccagttgt tccggcttca gctcgccacg
     4441 caccaccgct gaaaacagct ggtggctttc ttgttggcta agcgtctgcg cctgatacag
     4501 tttttccaga atcggttgca gcgtgttggc tggctctagt ttctgctgcg cccaggccag
     4561 cgtttgttcc agcaggcgag cgccctgggt ggtgagaatg gattccggat ggaactggaa
     4621 tccacaaacg cgatccgcat cgtgacgtac tgccatcacc atgccattaa aatgggcgtt
     4681 gatggttaaa ccggccggaa tgttactgcc aaccagcgag tgataacgcg ccaccggcag
     4741 cgggtttgtt aatccggcaa acatcgcctg accgtcatgt tcaatgctcg acgctttacc
     4801 gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa ttgcctgatg
     4861 tccgaggcaa atgccaataa tgggcagctt gccacgcaag cgggtgagga gttccggcat
     4921 acaaccggct tcgctcggca caccggggcc aggagaaagc atcagtaccg gattgctcat
     4981 cgtcgccagg cgttcaatta aggtctgcgc cggaatatgg ttgcggtaaa tcaccacatt
     5041 atgcccattg ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag
     5101 cagcagaatg tcagccatca gaaagtctcc tgtgcatgat gcgcggtggc aatagcgcgc
     5161 agtacagcgc gggctttatt acgggtttcg tcggcttccg actgcggaac agaatcaagg
     5221 actacgccag caccggcttg cacggtggcg ataccgtttt ccaccagcgc cgagcggatc
     5281 acaatgcagg tgtcgagatc gccatgcgcg gtgaaataac ctaccgcgcc gccgtagctg
     5341 ccgcggcggc gaccttccgc ctcggcaatt aactgcatag cgcgtacttt cggcgcacca
     5401 cttaacgttc ccatattcat acaggcgcgg taagcgtgca gggcgtcgag atcgtggcgc
     5461 agttcaccga ccacgcggga gaccaggtgc atcacgtaag agtaacggtc aactttggtg
     5521 aggtcggcga cgtagcggct gccgggggtg caaatgcgtg ccagatcatt acgggcgaga
     5581 tccaccagca tcagatgttc agaaagctct ttatgatcgg tacgcatttc cagttcgatg
     5641 cggctgtcga ggtctctgtc cagcgagcca tcggcacgac gaccgcgtgg acgtgttccg
     5701 gcaatcgggt agatctcaat ctggcggctg gtggcgtcat acttgagcga actttccggc
     5761 gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta
     5821 ctctttttca gcacgtaata ggccgccagc ggtgacgggc agggcagaga gaaacggcga
     5881 gatggcacca cctggaaaat ttctccagcg cgaatcgctt tttgcaacaa acgcactacg
     5941 ccaccgaact cttcatcgct ctgattacat tcacaacgca tatgcggcac ggaaaccact
     6001 ggcagcggcg gcgcggcttc ggtcagttgc tgacgtagtt cgttcaggcg agcagtgaga
     6061 cgttgttttt cttcttcatt cggagcaaac aggctggcct gaatgcgggt gctttttttc
     6121 tgatggtcaa tcaccatcag agtttcagcg agataaaaac agaaatcagg gcagttattt
     6181 tccgctgaca gttgcggtaa atcttcaaat cccgccacaa ggtcataaga gaacaggccg
     6241 ccgaagaaca ttgcttctcg ttcttccttc ggtacattca acagattctg caataaacgg
     6301 aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt catccagcag tggactgaca
     6361 ggggggaagc gcagcacacg gcagtttggt aattgttcat tttccacacc cgcaggcagg
     6421 gcgttatcca gtagtgtcag cagggcttcg ccgttgccgg aaagtgcctg gattgtgaca
     6481 gtgtcaccta aagctgtaat gcgcagcgca ctgtctacca gcagcaggct ttttaaatca
     6541 tctttgctgt cgatatctgc ggattccagc agcagcgttg ccggacgatc cccacacaac
     6601 tggtgaaaaa gcgcggtggg attgtcgcga taagcgcctt cgcaggttag cagttcgaga
     6661 gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt
     6721 aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt
     6781 gcgccaccaa cctttcagta cgaaaattgc tttcattgtc gatacccttt ttacgtgaac
     6841 ttgcgtacta gttaactagt tcgatgatta attgtcaaca gctcatttca gaatatttgc
     6901 cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc gccttgagcg
     6961 acacgaatta tgcagtgatt tacgacctgc acagccatac cacagcttcc gatggctgcc
     7021 tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca
     7081 ccgaccatga caccacagct gcaatcgcgc cagcaagaga ggaaatttca cgttcgggac
     7141 tggcgttgaa tcttattccc ggcgtggaaa tttccacggt ctgggaaaat catgaaattc
     7201 atattgtcgg gctgaatatt gatattactc atccgctaat gtgtgagttc ctcgcgcagc
     7261 agacagaacg gcgcaatcag cgggcgcagc tgattgccga aagacttgaa aaagcgcaaa
     7321 tacctggggc gctggaaggc gcacaacgac tggcgcaggg cggcgcagtg acgcgcggtc
     7381 attttgcgcg tttccttgtt gagtgcggca aagccagttc aatggcggat gtctttaaaa
     7441 agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca atagaacaag
     7501 ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct gggcggtaca
     7561 atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac cacggtgacg
     7621 cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg tacccagctg gcggcccttg
     7681 cgcgtcagca tcatttatgg gcatcacaag gatctgattt tcatcagcca tgcccgtgga
     7741 tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg cagctatggg
     7801 aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata ttcatcctga
     7861 taacccacag caacgtctga tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat
     7921 tgtttatcca actgattccg gctatgcgct cggctgtaaa attgaagata aaaacgcgat
     7981 ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc tgatgtgtcg
     8041 cgatctttct gaactctcga cctattcatt tgttgataac gttgcgtttc gtttaatgaa
     8101 aaacaacacg ccgggcaact ataccttcat cttgaagggg acgaaggaag tgccacgccg
     8161 tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccttctaacc cgattgcgca
     8221 ggcgttgctt gaagcactgg gcgaaccgat gctttccact tcgctaatgc tgccaggcag
     8281 cgaatttacc gaatccgatc ccgaagaaat taaagaccgt ctggaaaaac aggtggattt
     8341 gattatccac ggcggctatc tcggacagaa accgacaacg gttattgatc ttaccgatga
     8401 tacgccggta gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat aagtgcgtaa
     8461 ctctgtatac tacgcggcct taaaatggcg ctatacggcg ccattgattc tcctgttcga
     8521 cgcctgtgaa ggcgacacca aaaggaagct ctatgagcga aaaattacag aaagtgctgg
     8581 cccgcgctgg ccacggctcc cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga
     8641 gtgttgatgg caaaattgct aaactcggcg atcgcgttga agttacccct gggctgaaaa
     8701 ttcgtatcga tggtcatctg atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc
     8761 tggcctatta caaaccggaa ggtgagttgt gtacccgtaa cgatccggaa gggcgtccga
     8821 cggtgtttga ccgtctgcca aagctgcgtg gcgcacgctg gattgccgta ggtcgtctgg
     8881 acgttaacac ctgtggtctg ctgctgttca ccaccgatgg tgaactggca aaccgtttaa
     8941 tgcatccaag ccgtgaagtt gaacgtgaat acgccgtgcg tgtatttggt caggttgacg
     9001 acgcgaaact gcgtgatttg agtcgtggcg tgcaactgga agatggtccg gcagctttta
     9061 aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtg actctgaccg
     9121 aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt tggtgtgcag gtgagccgcc
     9181 tgatccgtgt tcgttacggt gatatcccac tgccaaaagg tctgccacgc ggtggctgga
     9241 cggagctgga cctcgcccag actaactatc tgcgcgaact ggtggagctt ccgccagaaa
     9301 ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat gaaggctaat cagattcgtc
     9361 gtgcggtgaa acgtcacagt caggtgagcg gcggtcgtcg ttctggcggg cgtaataacg
     9421 gttaatcaaa gtatgccgga cgtcatatcc ggcattttta cagattaata atcaatccct
     9481 atttgcgcct taacaccggc atcaaacgca tgtttgatgg ggcgcagctc acttaccgta
     9541 tccgccagtt caagaatatc ccgatgacag cctcgacccg tgataatcac cgtctgttga
     9601 tgtggacgtt catttaacgc ctgcaccact tcttccagtg gcaaatagtc atacgccacc
     9661 atatacgtca gttcatcaag caaaaccata tccagtgagg aatcagcaag catccgcttt
     9721 gcatgttgcc agacttcgcg gcaggcggcg gtatcagact cgcggttttg tgtatcccag
     9781 gtaaagcccg ttgccatcac ctgaaactca acgccatgtg gctccagcag attgcgttcg
     9841 ccattaggcc aggtgccttt aataaactgc acgacgccca ctttttttcc gtgaccaact
     9901 gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt ttccattgcc ggtaaagacg
     9961 atgataatac cgcgttcatc ctgggcctgg gccacacgag catctacttt ttctttcact
    10021 cgctgctgac gctgttggta gcgttcatca ctcattggga aattcctggt ttacggcccg
    10081 gttgggcgtc aaaggtcatg ccggttttac ggcggctgtc atcgcccatc agccagaggt
    10141 agagcggcat gatatcagcg ggtgttttaa gtttctgtgg atcttcggtc gggaaggcgc
    10201 ttgcgcgcat tgcggtgcgc gtaccgcctg ggttaatgca gttgacgcgc aggcgctgct
    10261 gatattcatc ggccagtacc tgcatcatcc cttcggtggc aaatttcgac gctgcatatg
    10321 caccccagtt agctcgtccc tgacgtccaa cgcttgatga agtaaagacc agtgaaccgg
    10381 cgtccgattt gagtaataaa ggaagcagtg cctgggtgag cataaaggtg gcattaacgt
    10441 tgacctgcat gacgtcctgc cagacctgcg gattttgttc gctcattggg caaatatcgc
    10501 cgagcaatcc ggcattatgc aaaacaccat ccagacgcgg ataattaacg acaatgcgct
    10561 gtgccagttg ttggcaatct tcggacgtgc aggtcagcaa atcgagaata aaccactgtg
    10621 gctgacgtcc agtttcttcg tttatgtggc tggctacctg acgtaatttt tcttcattac
    10681 ggcccaacag aatcactgtc gcaccatagc gtgcatacgt catcgcggct tcacggccaa
    10741 tcccatcgct ggctcccgtc accaggataa tgcgatcatt gagtaaatct tgttttggct
    10801 ggtaatgcat ggctactcct caacgacgtt gtctgtcgcg ctgccagtgt actttatgac
    10861 tttggggctt tatgcctgaa acagaagggc atttcaatca gcctggggcg ataacaccgc
    10921 aaaatggata ctttgtcata ctttcgctgc aataacatct ctgcgagacg gcttaacatg
    10981 cctgttgtaa actgtgagcc aaagcgttgt ttaaccaagg tggggactcg tggaattgtt
    11041 gtctgaatat ggtttgtttt tggcgaaaat cgttaccgtt gtgctagcga ttgcggcgat
    11101 tgccgccatt attgtcaatg ttgctcaacg taataaacgc cagcgtggcg agttacgggt
    11161 caacaatctc agcgaacagt ataaggagat gaaagaagaa ctggccgcgg cgctgatgga
    11221 ctcacatcag caaaaacagt ggcacaaagc gcagaagaaa aagcacaagc aagaagcgaa
    11281 agcagcaaaa gcgaaagcca aactgggcga ggtggcaact gacagtaaac ctcgcgtctg
    11341 ggtgctggat tttaaaggca gcatggacgc ccatgaagtg aactcgctac gtgaagagat
    11401 aacggctgta ctcgcagcat tcaaaccgca ggatcaggtt gtgctacgtc tggaaagccc
    11461 tggtggcatg gtgcatggtt acgggttggc ggcttcgcag ctgcagcgtc tgcgtgataa
    11521 aaacattcct ttaactgtta cggtagacaa agtcgctgcc agcggcggtt acatgatggc
    11581 ctgtgtggcg gacaaaattg tttccgcacc gtttgctatt gtgggttcca ttggggtggt
    11641 ggcgcaaatg cccaacttta accgcttcct gaaaagcaaa gatattgata tcgaactgca
    11701 cactgccggg cagtataagc gtactctgac gttgctgggt gaaaataccg aagaagggcg
    11761 ggagaaattc cgcgaagagt tgaacgaaac gcatcagttg tttaaagatt ttgtgaagcg
    11821 tatgcgtccg tctctggata ttgaacaggt ggcaacgggt gaacactggt acggacaaca
    11881 ggcggtagag aaaggcctgg ttgatgaaat caacaccagt gatgaagtta ttcttagcct
    11941 gatggaaggc cgtgaagtgg ttaatgtacg ctatatgcag cgtaaacgac tcattgaccg
    12001 attcaccggc agcgcggcag agagcgccga tcgattgttg ctacgctggt ggcagcgggg
    12061 tcaaaagcca ttgatgtaaa agacaaacgc gaggctaaga cctcgcgttt tgctttaatc
    12121 aaccagatga tatttttctg aaagcacatg ggccaggtgt ttgaacatat taaacaccgc
    12181 ggtgcttttg gctgttggca atccttgttc atctaaaaag tagtcgccgg taaataccag
    12241 cacgccatta cgctgcgtga cgccggtggc ttcaatccct gcgagcgtat cctcatgctc
    12301 acgaatgatt ttgttggcct ctttcaacag cgtttcgcgg tcgatgggtt gtgtctcttt
    12361 gttcattatt tactccttaa acaaggacat tagtctacgc caggcatggc ttgcagacaa
    12421 atataccacg ctggtggcaa gagcgcctta ctggcaactt tggattttgc atgctaataa
    12481 agttgcgtat cggattttat caggtacagt gtgacgcttt cgtcaatctg gcaatagatt
    12541 tgcttgacat tcgaccaaaa ttccgtcgtg ctatagcgcc tgtaggccaa gacctgttaa
    12601 ctcagtcacc tgaattttcg tgaacagagt cacgacaagg ggttgatatc cgcagagaac
    12661 gagtccatat cggtaactcg ttgccagtgg aaggtttatc aacgtgcgac gcattcctgg
    12721 aagaatcaaa ttaggtaagg tgaatatggg taaagctctt gtcatcgttg agtccccggc
    12781 aaaagccaaa acgatcaaca agtatctggg tagtgactac gtggtgaaat ccagcgtcgg
    12841 tcacatccgc gatttgccga ccagtggctc agctgccaaa aagagtgccg actctacctc
    12901 caccaagacg gctaaaaagc ctaaaaagga tgaacgtggc gctctcgtca accgtatggg
    12961 ggttgacccg tggcacaatt gggaggcgca ctatgaagtg ttgcctggta aagagaaggt
    13021 cgtctctgaa ctgaaacaac tggctgaaaa agccgaccac atctatctcg caaccgacct
    13081 tgaccgcgaa ggggaagcca ttgcatggca cctgcgggaa gtgattgggg gtgatgatgc
    13141 gcgctatagc cgagtggtgt ttaacgaaat tactaaaaac gcgatccgcc aggcatttaa
    13201 taaaccgggt gagctgaata ttgatcgtgt taatgcccag caggcgcgtc gctttatgga
    13261 ccgcgtggtg gggtatatgg tttcgccgct gctatggaaa aagatcgctc gtggtctgtc
    13321 tgccggtcgt gtgcagtcgg tggcggttcg cctggtggtc gagcgtgagc gtgaaattaa
    13381 agcgttcgtg ccggaagagt tctgggaagt cgatgccagc acgaccacgc catctggtga
    13441 agcgttggcg ttacaggtga ctcatcagaa cgacaaaccg ttccgtccgg tcaacaaaga
    13501 acaaactcag gctgcggtaa gtctgctgga aaaagcgcgc tacagcgtgc tggaacgtga
    13561 agacaaaccg acaaccagta aacctggcgc tccttttatt acctctacgc tgcaacaagc
    13621 tgccagcacc cgtcttggat ttggcgtgaa aaaaaccatg atgatggcgc agcgtttgta
    13681 tgaagcaggc tatatcactt acatgcgtac cgactccact aacctgagcc aggacgcggt
    13741 aaatatggtt cgcggttata tcagcgataa ttttggtaag aaatatctgc cggaaagtcc
    13801 gaataagtac gccagcaaag aaaactcaca ggaagcgcac gaagcgattc gtccttctga
    13861 cgtcaatgtg atggcggaat cgctgaagga tatggaagca gatgcgcaga aactgtacca
    13921 gttaatctgg cgtcagttcg tagcctgcca gatgacccca gcgaaatatg actccacgac
    13981 gctgaccgtt ggtgcgggcg atttccgcct gaaagcacgc ggtcgtattt tgcgttttga
    14041 tggctggaca aaagtgatgc ctgcgttgcg taaaggcgat gaagatcgca tcttaccagc
    14101 agttaataaa ggcgatgctc tgacgctcgt tgaacttaca ccagcccagc actttaccaa
    14161 gccgccagcc cgtttcagtg aagcatcgct ggttaaagag ctggaaaaac gtggtatcgg
    14221 tcgtccgtct acctatgcgt cgatcatttc gaccattcag gatcgtggct acgtgcgagt
    14281 agaaaatcgt cgtttctatg cggaaaaaat gggcgaaatc gtcaccgatc gccttgaaga
    14341 aaatttccgc gagttaatga actacgactt taccgcgcag atggaaaaca gcctcgacca
    14401 ggtggcaaat cacgaagcag agtggaaagc tgtactggat cacttcttct cggatttcac
    14461 ccagcagtta gataaagctg aaaaagatcc ggaagagggt ggtatgcgcc cgaaccagat
    14521 ggttctgacc agcattgact gcccgacttg tggtcgcaaa atggggattc gcacagcgag
    14581 caccggggta ttccttggct gttctggtta tgcgctgccg ccgaaagagc gttgcaaaac
    14641 caccattaac ctggtgccgg aaaacgaagt gctgaacgta ctggaaggcg aagacgctga
    14701 aaccaacgcg ctgcgcgcaa aacgtcgttg cccgaaatgc ggcacggcga tggacagcta
    14761 tctcatcgat ccgaaacgta agttgcatgt ctgtggtaat aacccaacct gcgacggtta
    14821 cgagatcgaa gagggcgaat tccgcattaa aggttatgac ggcccgatcg ttgagtgtga
    14881 aaaatgtggc tctgaaatgc acctgaaaat ggggcgtttc ggtaaataca tggcctgcac
    14941 caacgaagag tgtaaaaaca cacgtaagat tttacgtaac ggcgaagtgg caccaccgaa
    15001 agaagatccg gtgccattac ctgagctgcc gtgcgaaaaa tcagatgctt atttcgtgct
    15061 gcgtgacggt gctgccggtg tgttcctggc tgccaacact ttcccgaaat cgcgtgaaac
    15121 gcgtgcgcca ctggtggaag agctttatcg cttccgcgac cgtctgccgg aaaaactgcg
    15181 ttatctggcc gatgcgccac agcaggatcc ggaaggtaat aagaccatgg ttcgctttag
    15241 ccgtaaaacc aaacagcaat atgtctcttc ggaaaaagac ggaaaggcga ctggctggtc
    15301 agcattttat gttgatggca aatgggttga aggaaaaaaa taacctttaa ttctgtcagg
    15361 tttttataaa caaagggtcg cgaaagcggc ccttttttat tgcatattat tttttcttca
    15421 cacctataca ctaaggctat aaatgatata gtggttatag ttagcacctt ttttattatt
    15481 aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg gcgtctaagt
    15541 ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt caatcataac
    15601 ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat cagtaaacaa
    15661 gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg caagcacctg
    15721 acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgcgaagt cctgtcgaaa
    15781 gtcgatgcca taaaatcggt tgccggtgag cacacctggc cggataaagg ctcgctgtat
    15841 atcgccacca cgcataccca ggcacgctac gcgttaccaa acgttatcaa aggctttatt
    15901 gagcgttatc cccgcgtttc tttgcatatg caccagggct cgccgacaca aattgctgat
    15961 gccgtctcta aaggcaatgc tgatttcgct atcgccacag aagcgctgca tctgtatgaa
    16021 gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac tccggatcac
    16081 ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc gttggtgaca
    16141 tataccttcg gctttaccgg acgttcagaa ctggatactg cctttaaccg cgcagggtta
    16201 acgccgcgta tcgtcttcac ggcaacggat gctgacgtca ttaaaactta cgtccggtta
    16261 gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga tcccgacctt
    16321 gtgcgtgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg ttttcgccgt
    16381 agtactttct tgcgcagtta tatgtatgat ttcattcagc gttttgcacc gcatttaacg
    16441 cgtgatgtcg ttgatgcggc tgtcgcattg cgctctaatg aagaaattga ggtcatgttt
    16501 aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc ttttatgtag
    16561 cgaaggtgcc ggaatatatt ctcttttgtt acttatttct ataccgtgca caatattttt
    16621 atccctcata tttatagggt aaattacctg taaagtagtg tctaattacc cgcctcgcaa
    16681 actccagttt tcaaatatct atttcaggtc aaaagattga attattcact ggagacgatt
    16741 cgtaaattcg ctggattttt gcctaaagtt tctttaggat ttgtctcatc gtataattaa
    16801 ttacaccaat cgttcaacgt cttgctgata agtgatagcg attatatgag gttagcaatg
    16861 ccttctggaa atcaggaacc ccgccgcgat cctgaattaa aacgtaaagc ctggctggcg
    16921 gtttttcttg gttctgcact tttctgggtg gttgtcgcac tgctgatttg gaaagtgtgg
    16981 ggataactat ggtcggtcag gagcaactgg agtcgtcacc attatgccag catagtgaca
    17041 atgaaacaga aacgaaacgg gaatgttccg tcgttattcc agacgactgg caactaacat
    17101 cgcagcagca agcctttata gaactgtttg ctgaagatga tcagccgaaa caataattat
    17161 catcattctt attacccatt tttaatgaat tacagggctt ttaatacacc gcagcaataa
    17221 caggttgagt tatctcaaca caaagtaata accgttaagg gtgtagccta tgatcaacac
    17281 aaatatgaaa tattggtcct ggatgggcgc gttttctctg tcgatgctct tctgggccga
    17341 actcctctgg atcattactc actgatcctt gaccccgctg cggcggggtt gtcatttgct
    17401 ttgccacaag gtttctcctc ttttatcaat ttgggttgtt atcaaatcgt tacgcgatgt
    17461 ttgtgttatc tttaatattc accctgaaga gaatcagggc ttcgcaaccc tgtcattaag
    17521 gaggagctat gtcgtcaacc ctacgagaag ccagtaagga cacgttgcag gccaaagata
    17581 aaacttacca ctactacagc ctgccgcttg ctgctaaatc actgggcgat atcacccgtc
    17641 tgcccaagtc actcaaagtt ttgctcgaaa acctgctgcg ctggcaggat ggtaactcgg
    17701 ttaccgaaga ggatatccac gcgctggcag gatggctgaa aaatgcccat gctgaccgtg
    17761 aaattgccta ccgcccggca agggtgctga tgcaggactt taccggcgta cctgccgttg
    17821 ttgatctggc ggcaatgcgc gaagcggtta aacgcctcgg cggcgatact gcaaaggtta
    17881 acccgctctc accggtcgac ctggtcattg accactcggt gaccgtcgat cgttttggtg
    17941 atgatgaggc atttgaagaa aacgtacgcc tggaaatgga gcgcaaccac gaacgttatg
    18001 tgttcctgaa atggggaaag caagcgttca gtcggtttag cgtcgtgccg ccaggcacag
    18061 gcatttgcca tcaggttaac ctcgaatatc tcggcaaagc agtgtggagt gaattgcagg
    18121 acggtgaatg gattgcttat ccggatacac tcgttggtac tgactcgcac accactatg
//
LOCUS       DAPHDX010000088        18087 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPHDX010000088 DAPHDX010000000
VERSION     DAPHDX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.089"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1470
                     /gene="tnaA"
                     /locus_tag="RH058_003026"
     CDS             55..1470
                     /gene="tnaA"
                     /locus_tag="RH058_003026"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV3138583.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1561..2808
                     /gene="tnaB"
                     /locus_tag="RH058_003027"
     CDS             1561..2808
                     /gene="tnaB"
                     /locus_tag="RH058_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV3138584.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2940..4115
                     /gene="mdtL"
                     /locus_tag="RH058_003028"
     CDS             2940..4115
                     /gene="mdtL"
                     /locus_tag="RH058_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV3138585.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4090..5049
                     /gene="yidZ"
                     /locus_tag="RH058_003029"
     CDS             4090..5049
                     /gene="yidZ"
                     /locus_tag="RH058_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV3138586.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5206..5955
                     /locus_tag="RH058_003030"
     CDS             5206..5955
                     /locus_tag="RH058_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV3138587.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5977..6543
                     /gene="yieF"
                     /locus_tag="RH058_003031"
                     /gene_synonym="chrR"
     CDS             5977..6543
                     /gene="yieF"
                     /locus_tag="RH058_003031"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV3138588.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6597..7934)
                     /gene="adeP"
                     /locus_tag="RH058_003032"
     CDS             complement(6597..7934)
                     /gene="adeP"
                     /locus_tag="RH058_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV3138589.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8101..8766
                     /gene="yieH"
                     /locus_tag="RH058_003033"
     CDS             8101..8766
                     /gene="yieH"
                     /locus_tag="RH058_003033"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV3138590.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8833..9306
                     /locus_tag="RH058_003034"
     CDS             8833..9306
                     /locus_tag="RH058_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HDV3138591.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9355..9942
                     /locus_tag="RH058_003035"
     CDS             9355..9942
                     /locus_tag="RH058_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDV3138592.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10004..10726)
                     /locus_tag="RH058_003036"
     CDS             complement(10004..10726)
                     /locus_tag="RH058_003036"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV3138593.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGASKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10741..11910)
                     /locus_tag="RH058_003037"
     CDS             complement(10741..11910)
                     /locus_tag="RH058_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV3138594.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDECVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11937..13553)
                     /gene="bglH"
                     /locus_tag="RH058_003038"
     CDS             complement(11937..13553)
                     /gene="bglH"
                     /locus_tag="RH058_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDV3138595.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13639..15033)
                     /gene="bglB"
                     /locus_tag="RH058_003039"
     CDS             complement(13639..15033)
                     /gene="bglB"
                     /locus_tag="RH058_003039"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDV3138596.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKLYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15052..16929)
                     /gene="bglF"
                     /locus_tag="RH058_003040"
     CDS             complement(15052..16929)
                     /gene="bglF"
                     /locus_tag="RH058_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDV3138597.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17062..17898)
                     /gene="bglG"
                     /locus_tag="RH058_003041"
     CDS             complement(17062..17898)
                     /gene="bglG"
                     /locus_tag="RH058_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDV3138598.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4577 a         4514 c         4280 g         4716 t
ORIGIN      
        1 cagatgtaat attcacaggg atcactgtaa ttaaaataaa tgaaggatta tgtaatggaa
       61 aactttaaac atctccctga accgttccgc attcgtgtta ttgagccagt aaaacgtact
      121 actcgcgctt atcgtgaaga agcaattatt aaatccggta tgaacccgtt cctgctggat
      181 agcgaagatg tgtttatcga tttactgacc gacagcggca ccggggcggt aacccaaagt
      241 atgcaggcag cgatgatgcg cggcgacgaa gcctacagcg gcagccgcag ctactatgcg
      301 ttagccgagt cagtgaaaaa tatctttggt tatcaataca ccattccgac tcaccagggc
      361 cgtggcgcag agcaaattta tattccggta ctgattaaaa aacgcgagca ggaaaaaggc
      421 ctggatcgca gcaaaatggt ggcattctct aactatttct ttgataccac gcagggccat
      481 agccagatta acggctgtac cgtgcgtaac gtctatatca aagaagcctt cgatactggc
      541 gtgcgttacg actttaaagg taactttgac cttgagggat tagaacgcgg tattgaagaa
      601 gttggcccga ataacgtgcc gtatatcgtt gcaaccatca ccagtaactc tgcaggtggt
      661 cagccggttt cactggcaaa cttaaaagcg atgtacagca tcgcgaagaa atacgatatt
      721 ccggtggtaa tggactccgc acgctttgct gaaaacgcct atttcatcaa gcagcgtgaa
      781 gcagaataca aagactggac catcgagcag atcacccgcg aaacctacaa atatgccgat
      841 atgctggcga tgtccgccaa gaaagatgcg atggtaccga tgggcggctt gctgtgcatg
      901 aaagacgaca gcttctttga tgtgtacacc gagtgcagaa ccctttgcgt ggtgcaggaa
      961 gggttcccga catatggcgg cctggaaggc ggcgcgatgg agcgtctggc ggtaggtctg
     1021 tatgacggca tgaatctcga ctggctggct tatcgtatcg cgcaggtaca gtatctggtc
     1081 gatggtctgg aagagattgg cgttgtctgc cagcaggcgg gcggtcacgc ggcattcgtt
     1141 gatgccggta aactgctgcc gcatatcccg gcagaccagt tcccggcaca ggcgctggcg
     1201 tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa ttggctcttt cctgttaggc
     1261 cgcgatccga aaaccggtaa acaactgcca tgcccggctg aactgctgcg tttaaccatt
     1321 ccgcgcgcaa catatactca aacacatatg gacttcatta ttgaagcctt taaacatgtg
     1381 aaagagaacg cggcgaatat taaaggatta acctttacct acgaaccaaa agtattgcgt
     1441 cacttcaccg caaaactgaa agaagtttaa ttaatactac agagtggcta taaggatgtt
     1501 agccactctc ttaccctaca tcctcaataa caaaaatagc cttcctctaa aggtggcatc
     1561 atgactgatc aagctgaaaa aaagcactct gcattttggg gtgttatggt tatagcaggt
     1621 acagtaattg gtggaggtat gtttgcttta cctgttgatc ttgccggtgc ctggtttttc
     1681 tggggtgcct ttatccttat cattgcctgg ttttcaatgc ttcattccgg gttattgtta
     1741 ttagaagcaa atttaaatta tcctgtcggc tccagtttta acaccatcac caaagattta
     1801 atcggtaaca cctggaacat tatcagcggt attaccgttg ccttcgttct ctatatcctc
     1861 acttatgcct atatctctgc taatggtgcg atcattagtg aaacgatatc aatgaatttg
     1921 ggctatcacg ctaatccacg tattgtcggg atctgcacag ccattttcgt tgccagcgta
     1981 ttgtggataa gttcgttagc cgccagtcgc attacctcat tgttcctcgg gctgaagatt
     2041 atctcctttg tgatcgtgtt tggttctttc ttcttccagg tcgattactc catcctgcgc
     2101 gatgccacca gcaccactgc gggaacgtct tacttcccgt atatctttat ggctttgccg
     2161 gtgtgtctgg cgtcatttgg tttccacggc aatattccca gcctgattat ttgctatgga
     2221 aaacgcaaag ataagttaat caaaagcgtg gtatttggtt cgctgctggc gctggtgatt
     2281 tatctcttct ggctctattg cacgatgggg aatattccgc gcgaaagctt taaggcgata
     2341 atctcctcag gcggcaacgt tgattcgctg gtgaaatcgt tcctcggcac caaacagcac
     2401 ggcattatcg agttttgcct gctggtgttc tctaacttag ctgttgccag ttcgttcttt
     2461 ggtgtcacgc tggggttgtt cgattatctg gcggacctgt ttaagattga taactcccac
     2521 ggcgggcgtt tcaaaaccgt gctgttaacc ttcctgccac ctgcgttgtt gtatctgatc
     2581 ttcccgaacg gctttattta cgggatcggc ggtgccgggc tgtgcgccac catctgggcg
     2641 gtcattattc ccgcagtgct tgcaatcaaa gctcgcaaga agtttcccaa tcagatgttc
     2701 acggtctggg gcggcaatct tattccggcg attgtcattc tctttggtat aaccgtgatt
     2761 ttgtgctggt tcggcaacgt ctttaacgtg ttacctaaat ttggctaaat ccttcaagaa
     2821 gccagccatt cgctggcttc ttgcctctca ggaaatcact tatgtccaaa tggcaactcg
     2881 cctgatcctc cttcaccacg tatgctttgc gtcaccttac tatcaggacg ctttagccca
     2941 tgtcccgctt tttgatttgt agttttgccc tggttttact ttatcccgcc gggattgata
     3001 tgtacctcgt tggtttaccg cgcatcgccg ccgatctcaa tgccagcgaa gcgcagttgc
     3061 atattgcgtt ctccgtatat ctggcgggga tggcagctgc gatgttattt gccggtaaag
     3121 tggccgatcg ttcagggaga aagccggtcg ccatacccgg cgcggcgcta tttattattg
     3181 cctcggtgtt ctgttcactg gctgaaacca gcacgttatt tcttgcaggc cgatttctac
     3241 aggggttggg cgcaggctgt tgttacgtag tggcgttcgc cattttgcgc gacacgctgg
     3301 atgatcgacg tcgggctaaa gtgctgtcat tactcaacgg tattacctgc atcattccgg
     3361 tgttagcgcc agtgctcgga catctgatta tgcttaaatt cccgtggcag agtctgttct
     3421 gggcgatggc aatgatgggc atcgcggtac tgatgttgtc tttgtttatt ttaaaagaaa
     3481 cgcgcccagc gtcccccgct gcttcggaca aaccacgaga aaatagcgag tcgctgctta
     3541 atcggttttt cctcagccgt gttgttatca ccaccctcag cgtttcggtg atcctcactt
     3601 tcgtcaatac atcgccggta ttgctgatgg aaatcatggg ttttgagcgc ggagaatacg
     3661 ccaccattat ggcgttgact gctggcgtca gcatgaccgt ttcattctcc acgccatttg
     3721 cgctgggaat ttttaagcca cgtacgttga tgatcacctc gcaggtgtta ttccttgcag
     3781 cggggatcac ccttgccgtt tcaccttccc atgcggtttc tctgtttggt atcacgctga
     3841 tttgcgccgg tttctcggta ggttttggcg tagcgatgag tcaggcgtta ggaccatttt
     3901 cattacgcgc gggcgtagcc agctcgacct taggtattgc gcaggtttgc ggttcgtcac
     3961 tgtggatttg gctggcagcg gtggttggta tcggcgcatg gaatatgctg atcgggattc
     4021 tgattgcctg tagcatagtg agcctgttgc tgattatgtt cgtcgcgcct ggacgccccg
     4081 ttgccgctca tgaagaaatc catcaccacg cttgatctca atctgctgct ctgtctgcaa
     4141 ctgctgatgc aggagcgcag cgtgaccaaa gcggcgaagc ggatgaacgt gaccccttca
     4201 gcggtgagta agtcgctggc aaagttaaga gcgtggtttg acgacccgct ctttgtgaac
     4261 tcaccgctgg gcctgtcgcc cacaccgcta atggtcagca tggagcaaaa tctggcggaa
     4321 tggatgcaaa tgagcaatct gctgctggat aaaccgcacc accagacacc gcgcggcctg
     4381 aagtttgagt tggcagcgga atcgccgctg atgatgatca tgcttaatgc gctgtcgaaa
     4441 cggatctacc aacgttaccc gcaggcgacc atcaaactac gtaactggga ttacgattcc
     4501 ttagatgcca ttactcgtgg tgaagtggat attggttttt ccggtcgcga aagccatcct
     4561 cgctcgcggg agctgttaag ctcgctaccg ttagccattg attatgaagt gctgtttagt
     4621 gatgtaccct gcgtctggtt acgcaaagat catccggcac tgcatgaaac gtggaatctg
     4681 gacacctttt tacgttatcc gcatatcagc atttgctggg aacagagcga cacctgggcg
     4741 ctggacaatg tgttacagga gctggggcgc gaacgcacga ttgctatgag cctgccggaa
     4801 ttcgagcagt cactgtttat ggcagcgcaa cccgacaatc tgctactggc gaccgcgccg
     4861 cgctactgtc agtactacaa tcaactccat caactgccgt tggttgctct tcctctcccg
     4921 tttgacgaaa gccagcaaaa aaagctggaa gttcctttta ccctgctgtg gcataaacgg
     4981 aacagccata atccgaagat cgtctggtta cgggaaacca ttaaaaacct ttacgcgtcg
     5041 atggcataac cgaatcgtat gaaacgcgac ccaatttcac aataaaatgt aaaaaagttg
     5101 taataagctt gtctgaatcg aacttttagc cgctttagtc tgtccatcat tccagtaaat
     5161 gattactctt gtattcataa tggaccatta agcatggagc gaaaaatggc gactcacttt
     5221 gcccgaggga ttttaacgga aggacatctg atttctgttc gtctcccctc ccagtgtcat
     5281 caagaagccc gaaacattcc ccctcatcgt caaagccgtt ttctggcgtc tagaggttta
     5341 ctcgcagaac tgatgttcat gctgtatggc attggcgaat tgccggaaat cgtcaccctg
     5401 ccgaaaggta aaccggtttt cagtgataaa aatttgcctt cgttttctat ttcctatgcc
     5461 gggaatatgg ttggcgtggc gttaacaacc gaaggtgaat gtggtctcga tatggaacta
     5521 cagcgtgcga cgcgcgggtt tcatagccca cacgcgcccg ataaccacac cttttccagc
     5581 aatgaatcgc tatggatcag taaacaaaac gatcctaacg aagcgcgggc gcagctcatc
     5641 acgctgcgcc gaagcgtgct aaaactaacc ggtgatgttt tgaatgacga tccgcgcgat
     5701 ctgcagctgc tgcccattgc cggacgcctg aaatgtgctc atgtaaatca tgtagaagcg
     5761 ttatgcgacg cggaggacgt gctggtgtgg tccgtggcgg tcacgcccac gattgaaaag
     5821 ctcagtgtct gggagttcga tggcaaacac ggctggaaaa gcctgccgga tattcacagc
     5881 cgcgccaaca atcctaccag ccggatgatg cgttttgccc agctctctac cgtgaaggct
     5941 ttttcgccaa attgatagac aaccacagga gtcatcatgt ctgaaaaatt gcaggtggtt
     6001 acgttactgg ggagcctgcg caaaggctca tttaatggca tggttgcacg taccctgccg
     6061 aaaattgctc cggcgagcat ggaagtcaat gcgttacctt ccattgccga cattcccttg
     6121 tatgacgctg acgtacagca ggaagaaggt tttccagcaa cggttgaagc tctggcggaa
     6181 cagatccgtc aggctgacgg tgtggtgatc gtcacgccgg aatataacta ctcggtaccg
     6241 ggtgggctga aaaatgccat cgactggctt tcccgcctgc cggatcaacc gctggccggt
     6301 aaaccggtgt tgattcagac cagctcaatg ggcgtgattg gcggcgcgcg ctgtcagtat
     6361 cacctgcgcc agattctggt tttcctcgat gcaatggtga tgaacaagcc ggaatttatg
     6421 ggcggcgtga ttcagaacaa agttgatccg caaaccggag aagtgattga tcagggtacg
     6481 ctggaccacc tgaccgggca attgaccgca tttggtgagt ttattcagcg agttaagatc
     6541 taaataaaaa acccgccagc aatcatgcat ggcgggtttt taatgcgcta tcgattttaa
     6601 tgagcgtcga taaatacaat cttcaggata aacatcagcg caacgatgat tacgcacggg
     6661 ctaaggtcac gcagacgccc ggtaccaatc ttcatcacgc agtaggagat aaagcccagc
     6721 gcaatacctt cggtaatcga aaagctgaac ggcatcatca cggcggtaat aaacgccgga
     6781 acagattcag taagatcctg ccagttcacg cgagcaagac ttgaggtcat caacacgcca
     6841 acgtagatca acgcgccagc tgcagcgtag cctggcacca ttcccgccag cggcgacaga
     6901 aagataacca gcaggaacag cagaccaaca accactgccg tcagaccggt acgaccgcca
     6961 accgatacgc cggaagagga ctcaatataa gccgtaacgg aagaagtacc gataaacgaa
     7021 ccggtcacgg aagagatact gtcgacatac agcgcctgct tcatgcgcgg gaatttaccc
     7081 ttctcatccg ccaggcctgc tttatcggtc acgccaatca gcgtaccgga ggagtcaaac
     7141 aggttgacca gcatgaaaga gaaaatcacc cctgccagcc cgaggttaaa cgacccggct
     7201 aaatctacat ggcccacgac agtcattacg ctcggcggcg cagaaacgat gccattgtag
     7261 tggacatcac ccagcatcca gcccagcagc gttgtcacca cgatggaaac cagcaccgcc
     7321 gcgtgaatgt tgcgcgaggc cagaatagcg atgatgaaga agccgaggat ccccagcagc
     7381 acgctgtgag aagtcaggtt accgatgctg accagcgttt ccgggttagc gacaatcaca
     7441 cctgcattct tcagtcccat cataccaatg aacagaccga taccgctggt gatgcccaca
     7501 cgcagactta ccggaatgtt agcaatcatc cagtaacgaa cgcggaaaat cgtcagtaac
     7561 agcagaccaa tcgcgcccca gaagattgcg cccatcccga cttgccacgg caagcccata
     7621 gcctgtacaa cgacaaaagc gaagaatgca ttcaggccca tagcgggtgc cagtgcaact
     7681 ggcaggttag caaacagtcc catcataata ctgccgaatg cagcaatcag acaggtagta
     7741 acgaagacgg cgctggtatc catgccagca acgccaagaa tttgcgggtt aacaaaaacg
     7801 atgtaaacca tcgtcaggaa ggtggtaaaa ccggcgatca cttcggtccg tgccgtcgtg
     7861 ccatgttcgc gcagtttaaa cacgcgttcc agcatcccct ggccagaagc ctgggttgtg
     7921 tgttgttgac tcattatcta tttccgaaca aggagggaaa atccgtcgct atcgtatacc
     7981 aaaatgagac aatatggacg gctgaagata actttttttc attgtatttg cttatacggc
     8041 aacgattgcg tcatgcgaat tggcatgacg aaaacgttaa actaacgata aaggaaagac
     8101 atgtcccaga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     8161 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     8221 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     8281 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     8341 cggctgttcg attcagaact ggaagccatc gaaggggctg gagcgctcct gtcagcgatc
     8401 actgcgccaa tgtgtgtggt atctaacggc ccaaataaca aaatgcagca ttctatgggc
     8461 aagctgaata tgttgcacta cttcccggat aaactgttca gcggctacga tattcagcgc
     8521 tggaagccag acccggcgtt aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa
     8581 aactgcattc tggttgatga ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg
     8641 gaagtgttct acttctgcgc cgacccgcac aataagccga tcgttcaccc gaaagtcacc
     8701 acctttaccc atctttcgca gttaccggaa ctgtggaaag cgcgtggttg ggatattacg
     8761 gcatagttct tcacactccc ttcacttacc ccgcttaaat tggcgctcaa aggtaagtaa
     8821 agggagtttg atatgtctgt ttcacgtcgg gtaatacatc acggacttta ttttgcggtt
     8881 ttaggaccgt taattggtgt tctgtttctt gtcctctaca tattcttcgc aaaagaaccg
     8941 ctggttcttt tggtaataat acaggtactt ccattgtttc tcttattgtc gattactacg
     9001 ggagctattc ctgcattgtt aaccggcgtg atggtcgcct gtctgccgga aaagatcggg
     9061 tcacagaaaa attatcgttg tctggctggt ggcataggtg gcgtcgttat caccgagatc
     9121 tattgtgcag ttattgtaca tattaagggc atggcttcct cggagttgtt tgaaaacatt
     9181 ctttctggtg acagtctcgt tgtccgcatc attcctgcat tgctggcagg tgtggtgatg
     9241 agcagaatca ttacccgtct gcccggattg gatatttcat gtcctgaaac agactcttta
     9301 agttaagcgg gatactttat ctttgggcta ctcaaaagca gacaggatgt tactatgact
     9361 caaaatatca ggccgttacc ccaattcaaa tatcatccca agccactgga aacaggcgca
     9421 tttgaacagg ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac
     9481 tcgggtccct tttattgcgt tgatgaagtt gaacatctct gtccgtggtg tattgcggac
     9541 ggttctgctg ctgaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt
     9601 gaatttgagt atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa
     9661 atgctgaaag agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg
     9721 cttgcgcatt gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa
     9781 gatcgcctcg atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat
     9841 tctgatctag ctaaatgcct gcaaaagcgt ggtgattgtc agggttatct cttccgctgt
     9901 ctccactgcg gcaagctgag actgtggggt gatttttcgt agttatttaa ataatgagaa
     9961 caggccggag cgtaattcat acatccggcc ttatttcttc agcttaaccg agcgctaatt
    10021 ctgcggcagc ggctttatca gcgattacca tcagcgaagg atgcagctgc aacacggaag
    10081 ctggaacatc ttcggtaacc ggaccttgca gcacattttt cagtgcctgc gctttacttg
    10141 cgccactgac aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca
    10201 cgtagctgtc aggcactaac gaaaagtcgc cgcccaactc accatgtgcc acaatatcaa
    10261 ccatctctcc ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtattcggca
    10321 aattgccaca aaaatgacca tctgcaccta atcccagcac caccaaatcc aggccgcctt
    10381 cacgcgccag tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct
    10441 cttctttgat ccccgcaggg gtgaaaaaca gattacgcag attggtaatc gttacgcctt
    10501 ctccctcttt gccacgaaag ggaatttcat cgaaattata gaaatagcag ttatcgtacc
    10561 agggcttacc tttaaccagg gtggtgaggt attcatacat gcctttgggc gtgctaccgg
    10621 cggtaattgc caggttaaca cgacgcgtct tcgacatata accaagcaga tggtgtgccg
    10681 cgacacggct catttcctgg taatcttcgg taatgattaa tttcatctta atgccttatt
    10741 ttatttaaat aatttctgta cgaaggctgc ataggcaggc cgccagacat ccatttcatg
    10801 gttcaggccg ggatattcct ggtaatcaaa cttaattttt ttctgctcaa gctcagtttt
    10861 cagcccggcg atatccttgc cggttacgac atctttatca ccaacgacca cagtaaaatt
    10921 acgtagttgc tggttaatag ctgccggatc gttcagccgg gccgcgacac attcatccgg
    10981 tacggttgtc gtggtaacac cactgaatgt ggccagccag ccaaagcttt ccagatgatt
    11041 cattccggaa accagcgcct ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc
    11101 ggcatcttta cggacattaa aacgcttgct aatcagcggg ataatatcgt tcatcagttc
    11161 gcgatctgcc gctttagcat tcagcggata aaagacttta cgtctttcct gaggaacgaa
    11221 atcttcggga ataatgccct tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg
    11281 tttaattttc ccttcagcca gcaggttatc catgatttgc gggatacgcc cctgatcgat
    11341 agcggaacgt ccggtatcac caaaaccgtg atagaaatag agcactggca aaggctcgcc
    11401 catgccggtg tatcctggcg gggtccagac atacatctga cgttcagatt gcaatgcgtt
    11461 ggagtggtaa gttatggcga tcaaatcacc atgcgcaaca gaacgcgtat ccagataact
    11521 gcctggcacc agaatcatac tggagttaac ctggcgctga ggcttagtca ttgcggtgcc
    11581 tgtatcaatg ctgcgtacac catcaacatt gaaaaaatac tcgtacaggt tgcctttcag
    11641 gataggtgtg cgccacgacc agactcctgc ttcgtctttg gtcatcgggt gaatattgtc
    11701 cggaaccgga acacccacca ctacagagac attttttgcc ccaggggcaa agtagcgaaa
    11761 ggtcacgctg ttatcggcat tgacctgagt cacatactgt ttcaccggga tagtgggtgc
    11821 cgggctggct ggcatatccg ctgctatggc ccactgagca gaaataccgc tggcaatagc
    11881 cagcgttaaa gcagcaattt ttatattcat cgtaatattc ctttcattaa gttccattac
    11941 caccagattt cagcctgggc accgacagca aactggtcgt ctttattgtc ttcgaaggtg
    12001 aagccatcca gttcgttttc cagggctttg atatacgtgg cgtagaaacg aatttccgga
    12061 cgcgaggtca acatactggt attgacttta aaggtatgga agagcgtggt tttataacca
    12121 gactcattaa atttattact attcgcatct ttgttttgct gggtgaaata gcccagttca
    12181 acacctgtct ggttatattg gtcccaaata taggccgggc gaacaaccgc acgaatagat
    12241 tcgaaatcag agtgtgcgcc tgtttcgtag ctataaatat cgttaccgaa ggagtaaaca
    12301 atcgcgttag ccacaataaa atgatcgccg atataggctt cgccctgcga ggtcagacga
    12361 actgctgttc cgccggtgtg atcaccataa taacgaccat taaaggtggt aaatggactt
    12421 gcgccagcat aacggccaaa gttactggcg atagagttat tcgcaaccag gaaggagaat
    12481 tcgttgaagc cacctttatc aaatttctgc gttaaagatg tgccgaacat ccaggtatct
    12541 ttccagtcat aatacccgtt attatcctga ttatcttttt ccgatgcgct ttcgtttgcc
    12601 gtgacataac gaccactcac cattaaggtc gctttatccc ataacgggat atctttatag
    12661 cgtaaatcaa tagtattggt attaatctgc tgtttgtttt gcaggctgcg atcgtaatca
    12721 tcgacatctt cgcgaaccag cgcgatatca attttacccg gaccgacttt ccagttttcc
    12781 agacctacac ccgctgcggc atcagtacgc tgcgttttcc agtccagcat ctggatttca
    12841 attttcggcg caccgtgttt acccacccag aaatcagcct ctggcgcaaa gggcaggaaa
    12901 cctttggtgg taacgtacat atcggagaac tgcataaagt tctcgccacc ggcgttatcg
    12961 ccaaaccagc cggtagagta ctgctgacca acgttaccat ccatcatcac aacggcatca
    13021 acccgtttgc cgttttcgtt gtagacacgt tgttttaact gcaaatcaaa ccagccggag
    13081 tattcgttac caaagcggcc cagagaacca atcgcccatg atttaggtga accatgagag
    13141 gcggtccccc aaccagaacg gtaatagccg ttgtaactaa agccaatttc atctttcaca
    13201 aacttactga aatctttcag agtcatggaa gaataaatcg agcctgtctc actggcattt
    13261 ttctcttcat ttttgaccag tacagcatca ggttttgcgg cactactggt cgggaacgga
    13321 ttggcggcat acccttgatc attcgtactt acgctacgat tcaccgtcgc tggcgtatat
    13381 tttttcttct cttcatcttt atactttttg agttcgcttt gcgtttctct taacgctttt
    13441 tctaataact caaggcgatg ttccaccgtt aatgattcag ccaatgattg tgcagaaaag
    13501 gctaacggtg ccattaataa gatggcagag gtaataagat ttcgtctaaa cataatatcc
    13561 ctttatggtg caaagaaaga attaacgtat cgcatccaga ctgttctgaa tgcgacgata
    13621 attaaggtgc tttattggtt atttttttaa tgacagcccc cgcgttttga tcacttctgc
    13681 ataccatccg aagcttttct tacgcgttct tgtgaggctt ccttcgccat tatcatcacg
    13741 atccacataa ataaagccgt agagcttaga catttgtgaa tgagatgcac tgactaaatc
    13801 aattggcccc caactggtgt accccataat atccacacca tcggcaatcg cttcatttac
    13861 ctgtaccagg tgatcgttta aataggcaat tcgataatcg tcctgtatcg aaccatccgc
    13921 ttcaacgctg tcttttgcgc ctaatccgtt ctcgacaata aataacggtt tttgataacg
    13981 atcccaaagc gtatttaaca gaacccgtaa tccaaccgaa tcaatttgcc acccccactc
    14041 tgaacttttc agatgcggat tggggatcat attcagtatg ttgccctgcg catttttatt
    14101 aatgctttcg tcgtgggaaa cacaaccagt catgtaataa ctaaaagaga tgaaatcgac
    14161 ggtatgtttt aaatcttctg cgtcactttc agtcatctca atggtgatat tgtggtcgcg
    14221 gaagaaacgc tgcatatagc cgggatactg gccacgcgcc tgaacatcac caaagaacat
    14281 ccagcgccgg ttctcttcca tggcctgcaa catatcctgt ggctggcagg tgagggggta
    14341 aaccagccca ccgagaagca tattgccgat tttcgcttcg gggagcaggc tatgacaggc
    14401 tttaactgcc cgcgcactgg caaccagttg atggtggata gcctgataaa cttccgcctc
    14461 gccactctct tctgccagcc ccacgcccgt gaatggctcg tgtaacgaca tgttgatttc
    14521 attaaacgtc agccataacg ccactttatg ttggtagcga gtaaagaccg tgcgggcgta
    14581 atgctcgaag tgatcgatga ccgctcgatt agcccaaccg ccgtagtttt tcaccagccc
    14641 atatggcatt tcgtaatggg ataacgttac cagcggcttg atccccgcct gcgccatttc
    14701 atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc ggttcgactt cgtcgccctg
    14761 agggaaaatt cgcgcccagg caatggaaat acgcagacag gtgaagccca tctcggcaaa
    14821 taacgcgata tcttccgggt aacggtgata aaaatcgatg gcgacatctt tgatattctc
    14881 tttccccagg atgcgcggtt ccatttttcc cattacgcca tgaggctgta aatctgaggt
    14941 cgagatccct ttgccatctt cctgccaggc accttccacc tgattggcag ctgttgcgcc
    15001 accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc gttagcgaat
    15061 gatggataac agcggttcac ctgcgcttat ctgcgccgtg ccgtggggta atacgtccgt
    15121 aaaatcatcg ctattactga ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat
    15181 agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac ccacgttgac
    15241 gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg ataccgacat gaatcaggag
    15301 ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat
    15361 tcgacccgca accggagaac gcacttcacc aaccgagggc agaatggcga tacctttacc
    15421 caacaggcca ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa tctctcccgt
    15481 catcggtgaa cagataccgc cctgctcagg tggtgtaata acctctggtg ttttctcttg
    15541 cggggcaccc tgcgctggct gacgtttagc ggtgatgaaa tgaagcatca ccgtaccgac
    15601 aaatgcgcaa ccgatggcaa tgacaccgcc aataacgctg gcccagacgg tgaaatcaat
    15661 tcccgttgac gggatggttt gcatgaaggt gaaaatactt ggcaaaccaa aggagtacac
    15721 tttcgtttgc gcgtagccaa tgatggtggc ccccaaagcc ccactgatac aggcgataac
    15781 aaaggggtac ttacgcggca gattaacgcc atataccgct ggttcggtga taccaaacag
    15841 gcccgtcaac gccgctgatc ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa
    15901 gacgccgagc gccgccccga cctgcgccat aatggcgggc attaacagcg ggatcatggt
    15961 gtcgtagccc agcacggtga agttattgat acacagcggc accaggcccc agtgcagtcc
    16021 gaacatgacg aagatttgcc agaagccgcc cattaccgcg cctgcaaatg caggaaccgc
    16081 ctgataaagc cagagataac cggcggcaat cagttcgctt atccaggttg atagcggccc
    16141 caccagcaga aaggtgacgg gtgtgataac catcagacat agcaatggtg tgaagaaatt
    16201 tttgattgcc gacggtaacc acgcattaag tcggcgttcc agaatgctgc acaaccaggc
    16261 agaaaaaata atgggaataa ccgatgacga gtaattcaac aatgtgaccg gaatacccag
    16321 gaaatccagc cccagctcat ccgctttttg cccgttctcg aaagcagtca gaattaatgg
    16381 atgcactaac gctccaccaa tcaccatggc agtaaatgga ttaccgccga agcgtttccc
    16441 cgcggtgtat cccaggatta tcgggaagaa ccaaaacaag gcatcactgg cgctgaataa
    16501 aattaaataa gtaccacttt gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat
    16561 acctttcagg atcccggttg ccgccatcaa accgatcaga ggcgtaaaaa tacctgaaat
    16621 aacataaaca aagcggttta gcagattacc tttctcatca ttttccggtg cctgttgcgc
    16681 tttttcgcca aggcctgcca cgctgttgac cgccaggaag acatcggcca catggttacc
    16741 tatgaccacc tgaaactggc caccgctttc caccaccata ataataccgg gggtgttttt
    16801 cagtacctca gcttgcgctt tgctttcatc ctttaactta aaacgtaatc gcgttgcgca
    16861 atgcatcaga ctaacaatgt tatctgcgcc cccgactcct gcgactattt ttctggctaa
    16921 ctccgtcata acttgccctc taccgctttg cggcaaaact ccaaaaaaaa cctgaaaaaa
    16981 acggcctgac gtgaatcaag caattttttt caggttttgc ccgcttagtg cggtaacaat
    17041 cctttactca gtaataatat ttcagtgttc tttgcgcacg cgctctatat ttatcgctaa
    17101 aaacataatc tctgcgggtg agattttacg ttgatactgc aaaccaataa aaatggcgat
    17161 ccgttccgca cattgccatg cttgcgggta attttgtttt actgcttgtt gtaatgattc
    17221 atcactatcg ttaattgaag catgttcaag aatacgccat gataaaaact tcagatgcgt
    17281 aaccagtcgc tgataactca agctttcttc ctggtaatta aggctgaact gaaattttat
    17341 taattgcagc atttcgcgca ttaattgcgt gacacctgca acatcctcca tattcccact
    17401 catttgggca ctgaccagat gcatggcaat aaaacccact tcatctttcg gtaactgcac
    17461 gcccagccgt ttatcaataa tggttaacgc ttcttcccct agctggaact ctttcgggta
    17521 aagccgctgg atgtcccata gcagcgggtt aggcagcaac acgttttgct gaaagcgttt
    17581 aatcgcaaac tggcaatggt cagttagcga gatataaata ctgtcctgca actttcccag
    17641 gcgctcctgc gctaaagaga taatacgatc acaggttgcc atcacctcaa gaggaatatg
    17701 acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat actctttttc
    17761 tattccactt gagttaattc tttcgccagg gcgtttttga aagccaattc cgcgccccat
    17821 gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt tgagaatttt
    17881 ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaccc gacttcacca
    17941 gtattctctg gttatgtcag gttttgcctg cgaatgcagt aacaatccag tcatttatta
    18001 atgattttta taacgaacat ccaggttcga aaattaattt aattgcgtgc agagaaaata
    18061 gcaatgcgct attgataaaa atatgac
//
LOCUS       DAPHDX010000089        18006 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPHDX010000089 DAPHDX010000000
VERSION     DAPHDX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.090"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(181..1773)
                     /gene="lsrK"
                     /locus_tag="RH058_003042"
     CDS             complement(181..1773)
                     /gene="lsrK"
                     /locus_tag="RH058_003042"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV3138599.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1852..2805)
                     /gene="lsrR"
                     /locus_tag="RH058_003043"
     CDS             complement(1852..2805)
                     /gene="lsrR"
                     /locus_tag="RH058_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV3138600.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3054..4589
                     /gene="lsrA"
                     /locus_tag="RH058_003044"
     CDS             3054..4589
                     /gene="lsrA"
                     /locus_tag="RH058_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV3138601.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4583..5611
                     /gene="lsrC"
                     /locus_tag="RH058_003045"
     CDS             4583..5611
                     /gene="lsrC"
                     /locus_tag="RH058_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV3138602.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5611..6603
                     /gene="lsrD"
                     /locus_tag="RH058_003046"
     CDS             5611..6603
                     /gene="lsrD"
                     /locus_tag="RH058_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV3138603.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6615..7637
                     /gene="lsrB"
                     /locus_tag="RH058_003047"
     CDS             6615..7637
                     /gene="lsrB"
                     /locus_tag="RH058_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV3138604.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGILVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7664..8539
                     /gene="lsrF"
                     /locus_tag="RH058_003048"
     CDS             7664..8539
                     /gene="lsrF"
                     /locus_tag="RH058_003048"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV3138605.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8563..8853
                     /gene="lsrG"
                     /locus_tag="RH058_003049"
     CDS             8563..8853
                     /gene="lsrG"
                     /locus_tag="RH058_003049"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV3138606.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8910..9668
                     /gene="tam"
                     /locus_tag="RH058_003050"
     CDS             8910..9668
                     /gene="tam"
                     /locus_tag="RH058_003050"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV3138607.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLIESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9672..10586)
                     /locus_tag="RH058_003051"
     CDS             complement(9672..10586)
                     /locus_tag="RH058_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV3138608.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10793..12244)
                     /gene="uxaB"
                     /locus_tag="RH058_003052"
     CDS             complement(10793..12244)
                     /gene="uxaB"
                     /locus_tag="RH058_003052"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV3138609.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12471..13889)
                     /locus_tag="RH058_003053"
     CDS             complement(12471..13889)
                     /locus_tag="RH058_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3138610.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14028..14387)
                     /locus_tag="RH058_003054"
     CDS             complement(14028..14387)
                     /locus_tag="RH058_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV3138611.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14387..15313)
                     /gene="glsB"
                     /locus_tag="RH058_003055"
     CDS             complement(14387..15313)
                     /gene="glsB"
                     /locus_tag="RH058_003055"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV3138612.1"
                     /translation="MAVAMDNAILGNILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15377..16765)
                     /gene="sad"
                     /locus_tag="RH058_003056"
     CDS             complement(15377..16765)
                     /gene="sad"
                     /locus_tag="RH058_003056"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV3138613.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16866..17747
                     /locus_tag="RH058_003057"
     CDS             16866..17747
                     /locus_tag="RH058_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3138614.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17825..>18006
                     /gene="yneK"
                     /locus_tag="RH058_003058"
     CDS             17825..>18006
                     /gene="yneK"
                     /locus_tag="RH058_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV3138615.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIF"
BASE COUNT         4522 a         4604 c         4492 g         4388 t
ORIGIN      
        1 ataagggttg tgcttaatgc tttaagaaaa atagcaattt tcccttgaat atagtactgg
       61 tgatggaacg atgaatctgc aagctggctt ttaacaagcc agctctaaaa gaagggaaat
      121 aagaataact atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa
      181 ctataaccca ggcgctttcc ataacgacgt cgtcagtcca tgatcaacca gccccagctg
      241 atcctgataa actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg
      301 gtctggtgtg tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ccattgatga
      361 aaaaattccg gcaccgacgc cagctgcaat ggcacatcct aatgcagtgg cttctttgac
      421 caccggaata ttgacgggta atcccgagac atcagcgaga atttgactcc ataatttccc
      481 ttttgaacct ccgcctgcaa agactaacga tgaaggatga atattcgaga aatcagcaat
      541 ttgctgcaag ttacacgctg atacaatcgc cgcattttct tccagcgcac ggaacaatgt
      601 cgctttgtta catttatccg ggtcaatgga caagttaata aaggaaggcg cagcgtgata
      661 ccaggtttta aagcgcattc tgtcggagaa gatcggcatt acgccccacg acccaggcgg
      721 cacccgactg gccatctctt ccagcagcgt ataggtgtcg atgcctaaac gttcggcaat
      781 cagtttttct tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa
      841 gcttatagat tcagcttgta ccatgccagg aataacatga ggattaacgc gcacgttcat
      901 ttctgggtct gtcaccggcg cggctaaatt tacaacttgc tgccagaatg tgccgccaag
      961 aaccgcggtt tgtgccggac gcacaacgcc taacccaagg caaccaagct gcacgtcgcc
     1021 tcctccaacg accaccggag tgcccgcctt cagaccgcag agttccgccg cttgtgaact
     1081 taccacgccc agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgcaggcc
     1141 agccatatcc agcaatgcag gtttccagtc acgggtggtt agatcaagaa gtcccgtggt
     1201 gccagcgtta gagggatcca ccgccagttc gccgctgagc atataggcca gccagtcgct
     1261 gatcatggtg atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag
     1321 taatctgggg atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc
     1381 aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg cgtgccgctc tggcatccac
     1441 attggcgcag gcccagatcg gggctccttc attattatat aaaacaatgc cttcacgcat
     1501 cgaacatgcc gaaacggcag cgatatactc cggggctatg ccggcgttgt gcagcgcctg
     1561 gcgcatacac tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg
     1621 aacgtccggt actgccaggt gccgccactc cgcctgtccc actgctattt gattgccttc
     1681 caggtcgaat atcacagccc gaatacttcc ggtacctgca tccagcgcca tcaggtaata
     1741 ctttgattct gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg
     1801 tctgttcctc tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg
     1861 taaaatcgcc gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat
     1921 tgcagcggca attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat
     1981 ggtcttcagc gagcttaaag gtaagccaat cagttcgtta tgtattttaa tatccgtgac
     2041 aacgtcacct tttgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcgacc
     2101 aatcattaac tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcctg
     2161 ttgactgacg gccccaatgc cgacaatcgc cacatcggct gcttgcgcgg ctaacagaac
     2221 atctttgacg caattttcat tttttagcgt acgggcaatg tcagcggagg atgcccgcaa
     2281 cggagccgga ataatattca cactgcacgc cgcgttaagc tgcccgatac ccgtcatata
     2341 agaaccgacg ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact
     2401 taagcgttgc agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg
     2461 ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc agtcgcccgc cgacatcagc
     2521 atccgcaagc ccagggatga cccggacatg ttgcagcgaa aactgacgac gtaattgagt
     2581 ttcatattcc agacagcctt caaagcgaga attaatctgt acgcgaataa tgccggactg
     2641 atgccctttc tccagcaagc gcgacacttt caaacgggtc aggccgagac gatcgctgat
     2701 ctcgctctgg gtcagcccgt cgtgatagta aaaccacgcg atccgcgcga cctgttcttc
     2761 ttcacacatt ccctgttctg aaattgccga atcgttgatt gtcataattc attcttcact
     2821 ttgaacatat ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg
     2881 gcagccacag tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatagcat
     2941 aaattgtgat ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta
     3001 aaagcagaaa tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa
     3061 cgagtgatac ccgcgcgtta ccgctacttt gcgcccgctc ggtttataaa cagtattcag
     3121 gggtcaatgt cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc
     3181 tcggcggcaa tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg
     3241 ctgatagcgg tacgctggag attgggggca acaactacgc cagattaacg ccagttcatg
     3301 ctcatcagct gggcatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga
     3361 taaaagaaaa catcctgttt gggatggcaa aaaaacagct ctccatgcaa aaaatgaaga
     3421 acttgctggc ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg
     3481 tcgccgatcg ccaaatggtg gaaatcctcc gcgggctaat gcgcgactcg cggattctga
     3541 tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttactcgct
     3601 tgcaagagct gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa
     3661 ttcgccagat tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca
     3721 aaaccagcga actgtctacc gacgacatta ttcaggccat cacgccagtg gtacgggaaa
     3781 aatcgctctc tgccagccaa aaattatggc tggagttacc tggtaaccgc ccacaacatg
     3841 ctgccggaac gccggtgctg acactggaaa atctgaccgg cgaaggtttc aggaatgtca
     3901 gcatgacgct caatgccgga gaaattctgg gcctggctgg gctggtgggg gccggacgca
     3961 cagaactggc cgagacgctc tatggtctgc gtactttgcg tggcggacgc attatgctga
     4021 atggtaaaga gatcaataaa ttatccactg gagaacgttt actgcgcggt ctggtttatc
     4081 tgccggaaga tcgccagtca tccggactga atctcgatgc ttcgcttgcc tggaacgtct
     4141 gcgcccttac tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc
     4201 tggaacgtta tcgtcgggcg ctgaatatta aatttaacca accggaacaa gctgcacgga
     4261 cattatccgg tggcaaccag caaaaaatcc tcattgccaa atgcctggaa gcttcgccgc
     4321 aagcattgat tgtcgatgag ccgacgcgcg gcgtggatgt ctcggcccgt aatgatatct
     4381 accagctgtt gcgcagcatc gccgcacaaa atgtggcagt gctgcttatc tcctccgatc
     4441 tggaagagat cgaactgatg gcagatcgcg tgtatgtgat gcatcagggc gaaattgccc
     4501 actctgcact gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg
     4561 atagtcagcg tcaggaggtg tcatgctgaa gtttattcag aacaaccgtg aaatcacggc
     4621 actgctggcg gtggtgctgc tgtttgcatt acccggtttt ctcgaccgcc agtatttaag
     4681 tgtgcaaacg ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg
     4741 cgcgacgctg gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat
     4801 gtgcgcagtg ctgttgggga tgttactgaa cgcggggtat tcactacctg ttgcttgtgt
     4861 cgcgacttta ctgcttggtt tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct
     4921 aaagatccct gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt
     4981 gctgtggact ggcggtaaat ggattgaagg gttacccgcc gaactgaaac agctctccgc
     5041 accgctgctg tttggcgttt ccgcgattgg ttggttgacg ataattctgg tggcatttat
     5101 ggcctggctg ctggcaaaga cggcgtttgg acgcagtttt tatgccacgg gcgataattt
     5161 acagggcgct cgtcaactgg gtgttcgtac ggaagccatt cgcattgtgg cattttcgtt
     5221 gaacggctgc atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc
     5281 caaccagacc ggtaccgggc tggagatgaa agcaattgca gcctgcgtgc tgggcggcat
     5341 tagtttgctc ggcggttccg gtgcgatcat cggtgcggta ctcggcgcat ggttcctgac
     5401 gcagatcgat agcgtactgg tgctgttgcg cattccggca tggtggaacg attttatcgc
     5461 gggcctcgtg ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg
     5521 taatctacgg cggcaaaaat atgcccgctt tatgacgcca ccgccatccg ttaaacccgc
     5581 ttcgtcaggt aaaaaacggg aggccgcata atgcgtattc gctacggttg ggaactggct
     5641 cttgccgcac tgctcgttat tgagattgtc gcatttggtg caattaaccc gcgaatgtta
     5701 gatctcaata tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta
     5761 ccgctgacga tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc
     5821 ctctgcgcca ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgata
     5881 ctcctgacct tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat
     5941 accaaagtta acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagcgct
     6001 ctgctgcttt ccggtatggc cggagcgacg gggtacgaag gtattggtgg attcccgatg
     6061 gcgtttacag atttcgctaa cctggatgtg ctgggactcc ccgttccgct gattatcttc
     6121 ctgatatgtc tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaatgtg
     6181 tttttgattg ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgcacc
     6241 ttatgtgcgc tctatgccat gacggggctg gcgtctgcgg tcgccgctgt gctgctggta
     6301 tcgtattttg gttcagcacg ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc
     6361 gccgtggtgc ttggcggtgc caatatttat ggtggttccg gttccattat cggcaccgcc
     6421 attgcggttt tattagtggg atatttgcaa caaggtttgc aaatggcagg agtgccaaat
     6481 caggtgtcca gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt
     6541 agcctgcatc gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca
     6601 taaaggatat cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg
     6661 gcattgccgc aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac
     6721 tggttggcgt gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc
     6781 tgagcgttga tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt
     6841 tgattaataa cttcgtcaat caaggttata acgccattat cgtttctgcg gtttcgcctg
     6901 atggcttgtg tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg
     6961 actctgatac taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt
     7021 tgggaggtat attggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg
     7081 cgtttttcta ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag
     7141 cgaaaatcgc caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg
     7201 atgccactaa atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg
     7261 ccattatcgc ccccgacgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga
     7321 aaaatgacaa agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg
     7381 aacgcggcac ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag
     7441 tgtatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata
     7501 tccagggcgt aggtcaggtg gaagtctcgc caaacagcgt tcagggctat gactacgaag
     7561 cggatggtaa tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg
     7621 gcaaatacga tttctgatgt gcattactta accggagtaa gttatggcag atttagacga
     7681 tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa caaaatatcc cttttaccct
     7741 gaaaggttgc ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc
     7801 gaaaacgggt aataccgtga tgctggcctt tgatcatggt tattttcagg ggccgactac
     7861 cggacttgaa cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat
     7921 gtgtacgcgc ggcattttgc gcagcgtagt tcccccagcg accaataagc cggtggtact
     7981 gcgggcgtca ggtgcgaact ctattctggc ggaattaagt aatgaagccg tggcgttatc
     8041 gatggatgat gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag
     8101 cgaatatgaa catcagtcga tcaaaaatat tattcagctg gttgatgccg gaatgaaagt
     8161 gggaatgccg accatggccg tgactggcgt gggcaaagat atggtgcgcg atcagcgtta
     8221 tttctcgctc gcgactcgaa tcgccgctga aatgggggcg caaattatca aaacctatta
     8281 tgtcgaaaaa ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg
     8341 cggtaaaaaa ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg
     8401 cgcttctggt gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat
     8461 gaaagccgta caggcggtgg ttcaccataa cgaaacggct gatcgggcat atgaactcta
     8521 tctgagtgaa aaacagtaac tgcggattta aggagaagaa ttatgcacgt cacactggtt
     8581 gaaattaacg ttcatgaaga caagattgac gagtttatcg aagtttttcg ccagaaccac
     8641 ctgggctctg tacaggaaga aggcaatttg cgcttcgatg tcttacagga cccagaagtg
     8701 aattcgcgct tttatattta cgaagcctat aaagatgaag acgcagtggc attccataaa
     8761 accacgcctc actacaaaac ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt
     8821 aaaaaacgtc tgttcaatgg gttgatgccg tgaggcgaat ttatcatttt tatctacaat
     8881 tggggtaacg cgctgacggg agtaaaaata tgtctgactg gaacccctct ttatatctgc
     8941 actttgccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga
     9001 atatcaaata cgttgccgat cttggctgtg gcccaggtaa cagcaccgcc ctgctgcatc
     9061 aacgttggcc tgcggccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag
     9121 cgcgcagtgc tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg
     9181 aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg
     9241 aattgtttcc tcatctggtt tctttactta atccgcaggg cgtgctggca gtacagatgc
     9301 cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact
     9361 acccggatcg cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg
     9421 aagccggatg tgaggtcgat atctggcgaa ccacctacta tcaccagatg ccgtcacacc
     9481 aggcgattat cgattgggtg actgccactg gattacgtcc gtggttacag gatctgatcg
     9541 agagcgaaca gcagcttttt cttaagcgct accatcagat gctggaagag cagtatccac
     9601 tgcaagagaa cggacagata ctgctggcat ttccgcgtct ttttattgtt gcccggcgta
     9661 tggagtaaat atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttcg
     9721 gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt
     9781 aaatcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa
     9841 ataaaagtgt aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc
     9901 agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag
     9961 gcaaagggaa ttggcgtata ggcaatgcgc tcgcatcctg ccaggaccgc tgaaatatca
    10021 ttaagccgat cgttgaggct aataaacagg atatctgaaa gctgcccatt gcggcgctga
    10081 accgccaacc actcccccat tattaacaag atacggttag ctggagagtt cgaagccagt
    10141 acaggctgaa gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg
    10201 cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca accgggcaaa ctcccttaca
    10261 cttgccgaat ccggcaatgt cgtttttacc tcgcgcagta acgaccgtga ggcaatcatc
    10321 aactgccccc aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa
    10381 ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg
    10441 cccagatgcg tgtaccaggg cagcatgaaa ataacagcga tagaaaagag aaaattgagt
    10501 agtaagcgcg atgatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc
    10561 cagtgttgtt gtggacgaac aatcatggtt atcttcaggc gtgaaaaagt cgccctatta
    10621 aacgtgatta cgatcacatt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca
    10681 ttaacaggcg ctgcatgtgg catctgttaa tgataaaggc acataggatg ttgtaactaa
    10741 tatagtgaaa taagaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca
    10801 acggacgtac agcttcgcgc atcccttttt caagaatcgc atccaggtca ttagcaacct
    10861 gctcgaccag accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca
    10921 caatcgctac cagttcctgg gtaccgataa cacggtcacg atgctggctc cagagttgct
    10981 gataacgttc cagccagtgt gcatcgtcct gtaccggata tgtttctccg ttacgctcac
    11041 cacgatagaa ggcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgcctt
    11101 ttgccttctg ccctgccagc agctgcggca ggatgcgagt gcggaacttg gtcataccgt
    11161 tgagcgcgat agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg
    11221 cactggcgaa agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt
    11281 agatagcttt ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat
    11341 ccagccctgc ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca
    11401 ctttgcgttc tttgtacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt
    11461 ccagacgcag ttcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt
    11521 cagcggtgtc gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat
    11581 cgcgcggata accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat
    11641 ccaaccattg aataaatgct tctggcagtg cccactcttg agcatagcgc agcaccagtt
    11701 cacgcaacgc gtcgccattg tagtcgatca actcacacgg gatgatgatc caacctttat
    11761 ccagcgcacc gttgaaatgg ctaaaacgtt cgaacagcag acgggtcagt tttgcgggat
    11821 agcttaccgc tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg
    11881 tggtgttaga gaaaacaaag cgcatttccg gattgtgcgc cagtttcagg aattcatcgt
    11941 attcactgta gacgctgatt tcacgattaa cagagcgaat cagacgcgcg tcgctgaccg
    12001 cttccccctt ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca
    12061 gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg
    12121 tgtgctcatt caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac
    12181 caaactgaat gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt
    12241 tcacaatggg ttcccttctg attagtcata caacctgttt gaattggtac gacaggttag
    12301 caaactttaa tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt
    12361 atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa atacgccttt tcctggatca
    12421 taaagtggta gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact
    12481 tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga
    12541 cacgtatcgg cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg
    12601 cgaccactac cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat
    12661 ggttgcagtt caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc
    12721 accgacggca ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca
    12781 ccgacaatct tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt
    12841 ccatagttat cgttaatgct tttgaaatag tcaatatcaa gcagcatgac cgtcagatgt
    12901 tgtgtctgtt tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc
    12961 tgagtcagaa aatcaaagtc ggctcgcagc gcaacttgct tcattagcga attgatcgct
    13021 gccacgctaa aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg
    13081 gagaacattt ccggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc
    13141 accagtacga tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta
    13201 tagcgcactg cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca
    13261 atgactaccg aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct
    13321 ttaaagcgcg gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg
    13381 cccgttgaga attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca
    13441 atactgaccg aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa
    13501 ttaaatagcc gtaaggcact tactggctca tacttatttt tccccagacg cttatcccgc
    13561 acgaccagta aggcgacggt aacaataaac atcatattgg agaaattgat aaccagtgaa
    13621 accagtcccc attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca
    13681 tagtaatgca gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc
    13741 agcggccaga aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac
    13801 agcgtggtta gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaaa
    13861 cggaaagttg aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca
    13921 attatctcaa cgtaataagt aaattcacat aaaggatagc atgttcgcta acagcggcgg
    13981 caaataaagg taccctggga atgctatttt atcgttttcc gcaattctca tggctgattc
    14041 atctgtacaa ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt
    14101 aacgaaacac cctgcggaat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg
    14161 gtggcgtgct gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta
    14221 gccggtaatg ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact
    14281 ggcgcgcctt tctccaggca atactgacgc tctttcatgc ccagacgaaa gcgagagcga
    14341 aattttgaac gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac
    14401 gccctaactg tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat
    14461 catccagttc cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac
    14521 cgccaccaac gccagatttc gccggtagcc ccacccgcca ggcaaactcc cccgcgttct
    14581 ggtacatacc actggtcgcc atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca
    14641 caggttcatc aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca
    14701 gctctacaca gctcattttc agagcgcagt aatgaaagta gttttgcaga acggttgtca
    14761 catcatgatg gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg
    14821 aatgttcaaa ttcggaacgc gctaccaccg tatcgtatga aatatcagac acaccgctta
    14881 agccgcgcac gacttccagc atacgttgcc gtggtgcgct taatcgccct tgcaacatat
    14941 cgcaaaccac cagcgcacca gcattaataa acggattacg cggaataccc tgctccattt
    15001 ccagttgcac taaagaattg aacggtgatc cagacgggtc tttgccgacg cgttgccaga
    15061 tttcctcttc ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact
    15121 gaatggaaaa acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga
    15181 tagcaatgcc caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt
    15241 taccctgacc aatgagcggc cgcacttgcc gcaagatgtt tcctaaaatt gcattatcca
    15301 tggcgactgc cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag
    15361 agctgaatat gtcgcgtcag atccggtctt tccacaccgt ctggatatta cagaattcgt
    15421 gtaagccgaa atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca
    15481 ctcgcgcgtc gctggcacaa taaccattga taaacacccc accgcattcc agacgtgccg
    15541 ccatctgtct ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac
    15601 tatcattagc cagttccagc gcatgttctg catctttcgc aacggtgatt gccgcaacag
    15661 ggccaaacat ttcttcccga aacgcggtca tttctggggt aacattcgcc agaaccgttg
    15721 gcggatagta gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct
    15781 gcgccagggt tttctccacc tgatgatgca gctcatcacg taaatcaaaa cgagccattg
    15841 gtccgagagc gttctcttca tcacggggat cgcccatttt caaggctgcc gcagctgcca
    15901 caaaacgttc ggtaaatgca gaagcaattc cctcttcgat aataaagcgt tttgccgctg
    15961 cacaaacctg tccggtattc tgataacgtc cggctaccgc cgctttaacc gccagttcca
    16021 gatcggcatc gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt
    16081 tcagtgccgc tccagcctgt gcgccaatag ccgctcccgc acgaacactt ccggtcaccg
    16141 tgacagcagc aatgcgcgaa tctttaatca tttgactgac accgtcgttg tcggcattca
    16201 gccagccata tacgccttgc gggattcccg catctttaaa cacctgggca atgagctgag
    16261 cacagcccat cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg
    16321 gaaccgcgcc acgcatcacc tgccataacg gaaagttcca cggcataatc gccagaatcg
    16381 tccccaacgg tcgatactca ataactgcct gctgattttc caccagcgta ggttccgcct
    16441 tcagcattgc cggaccatgt tctgcatacc agtcacacaa attcgccgat ttcgccactt
    16501 cagcgcgcgc ctgattgatt ggtttgccca tttcacgggt gatcatttgc gccatttctt
    16561 cgctacgggc gcgcagagcc ttaccgatac cacgcagttt ttcagcacga taatctatat
    16621 ttgtctcgcg ccagtcgcga aagcctgctg ccgccagctg aagtgcgttt tcgatatcgt
    16681 tagcgccagc ccaaggcagc acagaaagtt gttcacccgt ggcaggattt attgaaattg
    16741 catgagttgc cggagtaatg gtcatcgggg tatctccttt atgagtcatg gtatgaagat
    16801 acgcagattt actcttgctt taaaatgaat aatattaagc cacttattca cgaatcgaga
    16861 atgttatgga tctgacccaa ctggagatgt tcaacgcggt tgccgaagct ggcagcatta
    16921 cccaggctgc agcaaaagtg catcgcgtac cgtcgaatct cactacccgt ctacgccagc
    16981 tggaaacaga actgggggta gatctgttta ttcgcgagaa tcagcgttta cgtctctctc
    17041 ctgccgggca taacttttta cgctatagcc aacaaattct cgcgttagtg gatgaagcgc
    17101 ggagcgttgt cgctggcgat gagccgcaag gtttattttc tcttggttcg ctggaaagca
    17161 ccgcagcagt gcgcattccg gccacgctgg cggaattcaa ccatcgttat cccaaaattc
    17221 agttttcact ttccaccggc ccttccggca ctatgctgga aggagtgctg gagggaaaac
    17281 tgaatgcggc atttattgat gggcccatta accatactgc cattgacggg ataccggtat
    17341 accgcgagga gctgatgatc gtcacgccac aaggtcatgc gccagtaatc cgcgccagtc
    17401 aggttaacgg cagtaacatt tatgccttcc gcgccaattg ttcgtatcgt cgccacttcg
    17461 agagctggtt tcatgctgac ggtgccgctc cgggaactat tcacgagatg gagtcgtatc
    17521 acgggatgtt ggcctgtgtg gtcgcaggag caggcattgc gcttattcca cgctctatgc
    17581 tggaaagtat gccggggcat caccaggttg aagcgtggcc gttagctgag caatggcgtt
    17641 ggttaacaac ctggctggtc tggcgtcgcg gtgcgaaaac gcgtccgctt gaggcattta
    17701 ttcaactact ggatgtgcct gactcggcaa aacagggtta ccaatgagct atttttgata
    17761 gttcttgcag ctcaaatgcc ctatatagtg atgttccgat gacttatgac tatatggggc
    17821 aaatatggtt acgccagtaa gcatcagtaa ttacatatct cttcctgatg attttcccgt
    17881 acgtaatatt gcacctcagg taaaagaagt tttaaaggac tttattgatg cgctaagtac
    17941 aataatatgt gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat
    18001 atttaa
//
LOCUS       DAPHDX010000090        17902 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPHDX010000090 DAPHDX010000000
VERSION     DAPHDX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.091"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..511
                     /locus_tag="RH058_003059"
     CDS             5..511
                     /locus_tag="RH058_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3138616.1"
                     /translation="MTCISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAATELSAIH
                     GHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKP
                     DTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAER
                     LLAGLPAH"
     gene            complement(521..1948)
                     /locus_tag="RH058_003060"
     CDS             complement(521..1948)
                     /locus_tag="RH058_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDV3138617.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1945..2652)
                     /locus_tag="RH058_003061"
     CDS             complement(1945..2652)
                     /locus_tag="RH058_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3138618.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2816..3793
                     /locus_tag="RH058_003062"
     CDS             2816..3793
                     /locus_tag="RH058_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDV3138619.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3863..4345)
                     /locus_tag="RH058_003063"
     CDS             complement(3863..4345)
                     /locus_tag="RH058_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV3138620.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4580..7162
                     /gene="leuS"
                     /locus_tag="RH058_003064"
     CDS             4580..7162
                     /gene="leuS"
                     /locus_tag="RH058_003064"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV3138621.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7177..7758
                     /gene="lptE"
                     /locus_tag="RH058_003065"
     CDS             7177..7758
                     /gene="lptE"
                     /locus_tag="RH058_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV3138622.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAECQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7758..8789
                     /gene="holA"
                     /locus_tag="RH058_003066"
     CDS             7758..8789
                     /gene="holA"
                     /locus_tag="RH058_003066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV3138623.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8791..9432
                     /gene="nadD"
                     /locus_tag="RH058_003067"
     CDS             8791..9432
                     /gene="nadD"
                     /locus_tag="RH058_003067"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV3138624.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9456..10067
                     /locus_tag="RH058_003068"
     CDS             9456..10067
                     /locus_tag="RH058_003068"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV3138625.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10327..10644
                     /gene="rsfS"
                     /locus_tag="RH058_003069"
                     /gene_synonym="rsfA"
     CDS             10327..10644
                     /gene="rsfS"
                     /locus_tag="RH058_003069"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV3138626.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10648..11115
                     /gene="rlmH"
                     /locus_tag="RH058_003070"
     CDS             10648..11115
                     /gene="rlmH"
                     /locus_tag="RH058_003070"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV3138627.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11146..13047
                     /gene="mrdA"
                     /locus_tag="RH058_003071"
     CDS             11146..13047
                     /gene="mrdA"
                     /locus_tag="RH058_003071"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV3138628.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13050..14162
                     /gene="mrdB"
                     /locus_tag="RH058_003072"
                     /gene_synonym="rodA"
     CDS             13050..14162
                     /gene="mrdB"
                     /locus_tag="RH058_003072"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV3138629.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14173..15261
                     /gene="rlpA"
                     /locus_tag="RH058_003073"
     CDS             14173..15261
                     /gene="rlpA"
                     /locus_tag="RH058_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV3138630.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15401..16612
                     /gene="dacA"
                     /locus_tag="RH058_003074"
     CDS             15401..16612
                     /gene="dacA"
                     /locus_tag="RH058_003074"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV3138631.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16723..16986
                     /locus_tag="RH058_003075"
     CDS             16723..16986
                     /locus_tag="RH058_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV3138632.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17087..17728
                     /gene="lipB"
                     /locus_tag="RH058_003076"
     CDS             17087..17728
                     /gene="lipB"
                     /locus_tag="RH058_003076"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV3138633.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4521 a         4503 c         4575 g         4303 t
ORIGIN      
        1 agcaattacc tgtattagcc aacccaagaa aacaacgttg cgtgataaag ccatggtgaa
       61 ttatgccttt gattatttaa gctcaccagg cagtctaccg ttaaccacgg cagccacgga
      121 gctttccgcg attcatggtc actcaacatc gcaatatcgc cttggcgaat tttatcttca
      181 tggtagcgac ggtaaaccac tggattatac acaggcgaga tactggtatg agcaatcagc
      241 agaacaggaa aatccgcgcg cacaaagcaa actggggtgg atctacctga aaggcctggg
      301 agtcaaaccc gatacccgta aagcaattct ctggtataag gaagcagctg aacaagggta
      361 tgctcatgct caatatactt tgggtttgat ctacagaaat ggcactggta ttaatgttaa
      421 ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa caacattaca aaaatgcgga
      481 aagattactt gccggacttc ccgcacatta acgtcgactg tcacccgaag agatggagta
      541 ttttccctat caaaccggca aagataaaaa tgtagaaaat aattttgatg acgcccagac
      601 agcccagccc gccatcgtca acgggcattg gctgcggttg cggctccggc tcgacggttt
      661 cctgaccggt aaaattcgcc gcctgaatta ataatcgacg aataattaat gcttccagtg
      721 gacaacgatc ttctgcatca aggcggctca caatcggcat taatgattct tctgtcggtt
      781 ggctggcctg cactaattgt ttggcaacta aatataatgg ccataaggga tgttctgtat
      841 cacgcgtgag tgagatccgc ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac
      901 gccccagcgc agagagtttt tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg
      961 cgctgtcgcc ggggacataa agtagctgtt taccctgatc gttaaccagt tcacgagctt
     1021 caatcatcat caccactaac gggtaataat cgacccgatg ctgacggcac cattgcaata
     1081 ttaaagcatc cgcctgggtg gagggatact cgcgccagta gtcacaaagt tctgccagca
     1141 agctttcgcc ttctccacaa tagacgcgtt gcgcgtattc ataataacgc gcatcttcat
     1201 tatcaggttc agcttcccgc cacgaccggg cgtaatccag aagttcgggg atatccaggc
     1261 gacctgcgct ataccagcgg agtaaatttc tgtgcagttt tgcatcatcg gggatgatca
     1321 ctgcgcgatg ttgcgctaaa tacaatgtca gccattctgg atgcgatgac caaatccgag
     1381 cgaacatttg gtaaaaatcg atggtgtcat tttgcacggc aacgggcaga tgcagaatgg
     1441 tctggaagtt aaaaacattc cccttcgcaa tggcatataa aaaggattcc agttcttctt
     1501 cgtctatttc cccgacgttt tcctcctgca accaccgcaa tctttctgct aataacacca
     1561 cgcactcgaa ggataaatgg gcagtgttca tggcgattgt gcacagcgac cagcgtaatt
     1621 catcaatctc ttccatcgag caataattta attgctgaat gaatcgctgc caggcggagg
     1681 gcagaaaacg ttcgctatca gaggcaagta acgcacgaaa ggcgagcaga atttcatgtt
     1741 ctgctacctc atattcttct ggttgccaaa cagatttagc aggcgactgc gcaatccgta
     1801 gcgcctcctc atacgcctga cgaagttgtt taaaaccctg cggatcggtt tctggatgaa
     1861 aggacggtaa cagcgccaga taagcgcggc ggataatatc gacgtcagtc gtttcctcta
     1921 tttcgagaat cttccagcaa tttttcatag gtcgctcata tccttaagcg tttccgggca
     1981 tgcagaatag taagccaatg gttgccagga caaggtaggg taatacgcat catcactggc
     2041 aaataattga taaaacatat cattgccgga atcgtcgtat tctttacgcg ctaagaattc
     2101 gcgtaataac tgcagatgat catcttcggg agactgccaa tattgccgac caaaggtata
     2161 accggcaaag tattgccgcc agttacgata ataacgtaga gcccgtagat gaatgcgcga
     2221 ctggatccat agactttctt cttcgctcaa ccagttatta agtacgccca ttcggcttaa
     2281 aaaacccatc cggacataat cccaggcttt aattcctccc tcaccacagc attcggcggt
     2341 gctggcgaca aattgcgcat aagcctgacc ttgctcattt agttcagcaa gatagtcacg
     2401 ccattcacat ggcgaataac gaaaccagcg gtgataaaac cctgccaacc gagcggcatg
     2461 accgttatcg atcattcggt gaattgtgcg cagcaggtca tcacgggcat caattcccca
     2521 ggaggaagaa aggtcaaaaa agcatgtacc taaatacatt ttgggtgatg aatgcgaagg
     2581 taaacagcat tcattgcgta tttccatcgg agtggaaagt gcaaacaata ttttttgcga
     2641 ctccatgtcc atataattta tccttaaata ttcattaaat gaatattagc cgcattgcga
     2701 ggctcgttta tttatacttt tctgacgcga cataatacgc ataaaataag gcttacgcaa
     2761 aaatggaaca cataaaaata taatatgttt tatctgcaac cataaggaaa tgataatgat
     2821 tttcacgtca acttgctgcg ataatttatc aatagatgaa attatcgaac gtgctgaaaa
     2881 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
     2941 ttctccggac gacgaaaaag ccttttactg gctgaagctg gccgccgagc aaggtcattg
     3001 tgaagcacaa tattccttag ggcaaaagta tactgaggat aaaagccgtc ataaagataa
     3061 tgagcatgcc atcttttggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
     3121 cgcccttggc tggatactgg atcgtggaga agaccccaat tataaagaag cggttgtctg
     3181 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta
     3241 cagaaatggc aacggagtcg caaaagacta tgcgctggca tttttctggt acaaacaagc
     3301 tgcattacaa ggccatagtg acgcgcaaaa caatctggcc gatctttatg aagacggaaa
     3361 aggcgttgct caaaacgaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
     3421 taatcggcat gctcagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
     3481 ggactataag caagcgatgt actggtatct gaaggctgcc gctcagggga gcgtcggcgc
     3541 ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc
     3601 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
     3661 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
     3721 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
     3781 agatttactg tagagataaa aacaagggcc ggggaatttc attcggccca tattctcttt
     3841 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcatgac
     3901 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
     3961 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
     4021 tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgatg tctgccagct
     4081 cctgccacaa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
     4141 tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc gtcagctcat
     4201 cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
     4261 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgca accagttcac
     4321 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
     4381 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat
     4441 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
     4501 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
     4561 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
     4621 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
     4681 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
     4741 taactacacc atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct
     4801 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
     4861 taccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
     4921 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
     4981 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc
     5041 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
     5101 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
     5161 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
     5221 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
     5281 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
     5341 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
     5401 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
     5461 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
     5521 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
     5581 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
     5641 gaacatcaaa ccagttatcc tggctgctga cggctctgag ccagatcttt ctcagcaagc
     5701 cctgactgaa aaaggcgtgc tgttcaactc tggcgaattc aacggtcttg accatgaagc
     5761 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
     5821 ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat
     5881 ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct
     5941 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
     6001 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
     6061 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
     6121 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
     6181 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
     6241 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
     6301 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
     6361 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
     6421 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
     6481 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
     6541 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
     6601 gaaactggtt tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
     6661 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga
     6721 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
     6781 caaactggcg aaagcaccaa ccgatggcga gcaggatcgc gctctgatgc aggaagcgct
     6841 gctggccgtt gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca
     6901 ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
     6961 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
     7021 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
     7081 ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac caggtaaact
     7141 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg
     7201 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag
     7261 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
     7321 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
     7381 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
     7441 gtattccgta acggtcaaac agcagagtgt cagatgatca tgacggtgaa tgcgaccgtg
     7501 ttgatccccg gccgtgatat ctacccgatt agcgccaaag tcttccgttc gttctttgat
     7561 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
     7621 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
     7681 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
     7741 accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
     7801 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
     7861 aggacgctgt tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca
     7921 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
     7981 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
     8041 aacaacttct cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta
     8101 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgcgcttgcg aatcgcagcg
     8161 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg
     8221 caaaacagct caacttagaa ctggatgacg ctgcaaatca ggtgctctgc tactgttatg
     8281 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg
     8341 gcaaattgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
     8401 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
     8461 aactgcgtct ggaaggcagc gagccggtta ttttgttgcg cacattacaa cgtgaactgt
     8521 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
     8581 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagcc
     8641 agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
     8701 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
     8761 ccctggcgga cgtatttatt gacggttgat atgaaatctt tacaggctct gtttggcggc
     8821 acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc gaatttgatt
     8881 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
     8941 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
     9001 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
     9061 gagtggcggc aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca
     9121 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
     9181 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
     9241 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
     9301 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
     9361 ggtgaatcgt gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc
     9421 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
     9481 gcgaaacgca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg
     9541 cacgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt tcctttgatc
     9601 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc
     9661 gccagctccc cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga
     9721 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
     9781 actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
     9841 aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta gtcgtcagcc
     9901 atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc gaggctatgt
     9961 ggcattttcg cgttgaccag gggtgttgga gcaccattga tatcaaccaa aaattcgcga
    10021 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt
    10081 ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc tccgcagcca
    10141 gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
    10201 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
    10261 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
    10321 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
    10381 ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc
    10441 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
    10501 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
    10561 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
    10621 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
    10681 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
    10741 cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc
    10801 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
    10861 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
    10921 aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc
    10981 tgtaaagcgg cggcggagca gagctggtcg ctgtcgacgc ttaccctccc ccatccgctg
    11041 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
    11101 tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac
    11161 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
    11221 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
    11281 cgctttaccg actaccagac tcgctctaat gaaaaccgca ttaagctggt gcctatcgcg
    11341 cccagccgcg gcattatcta cgaccgtaat ggtatccctc tggccctcaa ccgcactatc
    11401 taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct ggacgctttg
    11461 cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
    11521 tcacaccgtt tcacctctat tccggtgaaa actaacctga ccgaagtaca agtagctcgc
    11581 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
    11641 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
    11701 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc
    11761 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
    11821 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
    11881 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
    11941 ctggcgggta gccgcgcagc tgtggttgtc accgatccgc gtacaggtgg ggtgctggcg
    12001 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
    12061 tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
    12121 tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggggtg
    12181 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg ttcggaaaaa
    12241 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
    12301 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
    12361 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
    12421 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
    12481 ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg
    12541 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttcctca tttgctgatg
    12601 agcaccgccg aagacggcaa acaggtgcca tgggtacagc cgcatgaacc gcccgtcggc
    12661 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
    12721 cctaacggta cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatcc
    12781 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    12841 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    12901 gctgtcgcca tgattctgga gaacggtggt gcgggtccgg cggttgggac actgatgcgc
    12961 cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca
    13021 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    13081 gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca
    13141 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg
    13201 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    13261 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    13321 ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt gttcgttttc
    13381 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    13441 acgtttgccc gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca
    13501 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    13561 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    13621 tggcggcgtt cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    13681 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    13741 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt
    13801 cacagcttga atttctcccc gaacgccata ccgactttat cttcgcggta ctggcggaag
    13861 agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg atcatgcgcg
    13921 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggtggcttaa
    13981 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    14041 ttgtaggggt tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg
    14101 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    14161 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    14221 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    14281 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    14341 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    14401 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    14461 tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct tccgatcccc
    14521 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    14581 cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc tgaccgtctt
    14641 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    14701 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    14761 cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt
    14821 cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    14881 agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg cagcgaaccg
    14941 acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca
    15001 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    15061 gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    15121 gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg ccccttcgcc
    15181 agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    15241 tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    15301 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    15361 cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    15421 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    15481 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    15541 gtcctacatc ctgattgact ataactccgg caaagtgctc gctgaacaga acgcagatgt
    15601 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    15661 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    15721 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    15781 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat
    15841 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    15901 cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca
    15961 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    16021 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    16081 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    16141 caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatctctgc
    16201 ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    16261 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    16321 accggtttgg tttggtgatt ctgatcgcgc ttcgttaggt gttgataaag acgtgtacct
    16381 gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    16441 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    16501 caaaacgatc gaacaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    16561 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    16621 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    16681 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    16741 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    16801 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    16861 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    16921 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    16981 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    17041 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    17101 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga
    17161 attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc
    17221 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    17281 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccggggc aacaggtgat
    17341 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    17401 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    17461 cgcgccaggt gtctatgttg gggaaaagaa aatttgctca ctgggtttac gtattcgacg
    17521 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    17581 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    17641 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    17701 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    17761 tcattactca taatacctgc taatacttta catctccttc gcgtttgtat attttcttta
    17821 tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg attaacctat
    17881 tgaaatagtt gattatggtg ag
//
LOCUS       DAPHDX010000091        17697 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPHDX010000091 DAPHDX010000000
VERSION     DAPHDX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.092"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(500..2104)
                     /locus_tag="RH058_003077"
     CDS             complement(500..2104)
                     /locus_tag="RH058_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV3138634.1"
                     /translation="MNPDYVQSLQTSHHFPADLTYRLFPSELAYLIDDLYESTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMNPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKVVNRGYPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALVEGLNEHPEAGVISDEAVTFFRSYLKNYPGLLNKAWSGQ
                     PFDFGRADEKYHIAPRLTFSLMSQPDVFTNYINKNDVLAWGSGFLSRFLFSQVVSSSW
                     IRDYTRGEFRTISTLENFHKKINGFLLNHNINFPGMSTERKTLKLAKKALGEWQENQI
                     KIERKALAGGEWEHIRDIVLKAGSNILRIAGIFTCYCYKDAEEIESIALFKAMHLMDW
                     YLEEASTIFYPMSARCQFEQDACELYAWIMTRIRQNNWRAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLICQGYLCIIRMRSHQALYVSVYCNNGYLLPLGAMYYEQFDIVLPENIHK
                     AKAYHVNIPPELIPSFASPFSEYSLF"
     gene            2537..3193
                     /locus_tag="RH058_003078"
                     /pseudo
     CDS             2537..3193
                     /locus_tag="RH058_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3287..4018)
                     /locus_tag="RH058_003079"
     CDS             complement(3287..4018)
                     /locus_tag="RH058_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3138635.1"
                     /translation="MNQPIHNAYWLSRFESILNSALVQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSDTRKASDKKSGGSAVLWVKRGDVEAMQKAMERACYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
     gene            complement(5537..6667)
                     /locus_tag="RH058_003080"
     CDS             complement(5537..6667)
                     /locus_tag="RH058_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138636.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLTELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDALSELYAIYSWVSYCKRGDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGALGHNDSKSLYKTVLSVC
                     GGKRETEMFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNAFKENLFFVEIVSCCFLVEFFHDIDSFFINQRLSKLGSFIDKWREGKEIWIKVYLQ
                     LIHTTLGITETEKLVNLYNSFFTDKVVLWDESVKKVKWIYEK"
     gene            6852..7736
                     /locus_tag="RH058_003081"
     CDS             6852..7736
                     /locus_tag="RH058_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV3138637.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDAAITRFSFPAWILQLLR
                     KTRDRIIHLLVTLWDHLF"
     gene            7855..8532
                     /locus_tag="RH058_003082"
     CDS             7855..8532
                     /locus_tag="RH058_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138638.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYARKYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVIVTVGADGNATGA
                     SVEFIGK"
     gene            8538..8771
                     /locus_tag="RH058_003083"
     CDS             8538..8771
                     /locus_tag="RH058_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV3138639.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            8861..9679
                     /locus_tag="RH058_003084"
     CDS             8861..9679
                     /locus_tag="RH058_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3138640.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSEQY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDKHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLENLR"
     gene            9756..10241
                     /locus_tag="RH058_003085"
     CDS             9756..10241
                     /locus_tag="RH058_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV3138641.1"
                     /translation="MTTSSHNSTTPSVSVTAASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            10257..10733
                     /locus_tag="RH058_003086"
     CDS             10257..10733
                     /locus_tag="RH058_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV3138642.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VILAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            10802..11023
                     /locus_tag="RH058_003087"
     CDS             10802..11023
                     /locus_tag="RH058_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV3138643.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            11042..11686
                     /locus_tag="RH058_003088"
     CDS             11042..11686
                     /locus_tag="RH058_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDV3138644.1"
                     /translation="MSRIITTTVYTLNELSSTAREKARDEYRQHHADSNWYENVYEDF
                     REVCDIFGIDLRQRVIRLSNGRFMEEPCIWFSGFCSQGDGACFEGLWHWQPAAPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQVMT
                     TDAEAAVSEVLRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            11736..12104
                     /locus_tag="RH058_003089"
     CDS             11736..12104
                     /locus_tag="RH058_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV3138645.1"
                     /translation="MSDSLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELSPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYLAVYPTSETKT"
     gene            12194..12571
                     /locus_tag="RH058_003090"
     CDS             12194..12571
                     /locus_tag="RH058_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV3138646.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            12568..13056
                     /locus_tag="RH058_003091"
     CDS             12568..13056
                     /locus_tag="RH058_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138647.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITAKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLIVTLIRHYGVSLIHLDACAECLPGLPTF
                     DW"
     gene            13074..13271
                     /locus_tag="RH058_003092"
     CDS             13074..13271
                     /locus_tag="RH058_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV3138648.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            13356..14201
                     /locus_tag="RH058_003093"
     CDS             13356..14201
                     /locus_tag="RH058_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV3138649.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVLNVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPN
                     VLTER"
     gene            14270..14665
                     /locus_tag="RH058_003094"
     CDS             14270..14665
                     /locus_tag="RH058_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDV3138650.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITEALDRLNIRYSLIGATECYNKGQ
                     STQIPVSPEFKTPPRFKRVLVVGKSKINA"
     gene            14658..15605
                     /locus_tag="RH058_003095"
     CDS             14658..15605
                     /locus_tag="RH058_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDV3138651.1"
                     /translation="MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETH
                     TLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQNGRNRKRKIRKEIVQIITDVLH
                     NSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
                     DIYSLVTELTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHI
                     YDNYCIVREKGVNVPVLKNFVQICIQQDIERYGNQHPQFRNSPVDELKKGLDALRDDP
                     VHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN"
     gene            16009..17271
                     /locus_tag="RH058_003096"
     CDS             16009..17271
                     /locus_tag="RH058_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV3138652.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(17536..>17697)
                     /locus_tag="RH058_003097"
     CDS             complement(17536..>17697)
                     /locus_tag="RH058_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3138653.1"
                     /translation="IFQWLTGIRTENIPPFNPLINEDNETVMVFNGEKIVPADGLKSQ
                     PVILPDKGK"
BASE COUNT         4908 a         4412 c         3882 g         4495 t
ORIGIN      
        1 aaaaaatatc aaatcatttt tcttcactct cctattgaaa tgcaaacttg aacagcatct
       61 cttttcagta acagtgataa ccattcgtta ttcaggcaga aaccgacatt atcatataca
      121 cctgcactca tcacgacgaa aatcaaacga agttatatat gaaaactcac acaatatggt
      181 taatgattag ctttaatcta tgttaaaaat aatctttcga ttcactttcg atcaataaaa
      241 aacgaaagcc atgcttccac cagtgcgcac actcctgtat tcaaccagct ctgacagcag
      301 gtcattatga aaacaaaatt aaatcacaga tgtggtgcat gtgctgctac tgttgctacc
      361 gtagcggttt tttcagagaa attctcattc accccccata attggtgaac atttgcatag
      421 gtgcaggata tgcaaaacgc cttatcacca acctccgaat gaaggttggt tgattttgtt
      481 ttatctgtgt aaggttattt caaaataagc tatactctga aaaaggtgac gcaaatgacg
      541 gaattaattc cggtggaata ttaacgtgat atgctttcgc tttatgaata ttctcgggta
      601 gaacaatatc aaactgttcg taatacattg cgcctaaagg aaggaggtaa ccattgttac
      661 aataaacaga aacataaagt gcctgatgcg atcgcattct gatgatacat aaatacccct
      721 gacagattaa ctgattgagt acaggtgtaa gtttttctgc tcttctcagt cgattgggac
      781 catatctttc aatgtctgtt ttcctgatag cacgccaatt attctgcctt attcgggtca
      841 taatccatgc atacagttca caggcatcct gttcaaactg gcatcgtgca gacatgggat
      901 aaaatattgt gctcgcctcc tccagatacc agtccatgag atgcatagct ttaaaaagcg
      961 caattgattc aatttcctca gcatctttat agcaatagca ggtgaatatt ccagctatcc
     1021 tcagtatatt agaacctgct ttcagaacaa tatctctgat gtgttcccac tccccgcctg
     1081 caagcgcttt tctttcaatc ttaatctggt tttcctgcca ctcccccaac gctttcttcg
     1141 caagttttaa tgtcttcctt tcggtgctca tgccggggaa attaatgttg tggtttaaca
     1201 ggaatccgtt aatcttttta tgaaagttct ccagggttga tattgttctg aactcacctc
     1261 tcgtataatc acgtatccag gaagaactta caacctgact gaacagaaac cgggaaagaa
     1321 atccgcttcc ccacgccagt acgtcatttt tatttatata attcgtaaag acatccggct
     1381 gagacattaa cgaaaatgtc agacgtggcg cgatatggta tttctcgtca gcccgtccaa
     1441 aatcaaacgg ttgcccactc catgctttat tcaacaggcc cggataattt ttcagatagc
     1501 ttctgaaaaa agtgaccgcc tcatcagaaa taacccctgc ctcaggatgt tcattgagcc
     1561 cttccacaag cgctttaagc gaaacatctt catagataaa attcggtcga accggacgtg
     1621 tcggtttatt tctttcgtga ttacgcagcg cctcttcttc ctgttccccc ggataccccc
     1681 ggttaacaac ctttcttaaa ttggcagcaa gcgctttctg ccgggtattc cagatctgta
     1741 gttcagtctt ataatctttg tttctctctt cgtattgttg aatgagtcga tcggcaaatt
     1801 cgtaacaggg gttcatcacc agtctgttta tcgttgtttt tcccgcgcct ggctctgcga
     1861 ttgccaacag ataaagtgag cagggttccg gcatgttggt gtgaggatga acaacgtcaa
     1921 ccaatgactg acaggagagt gagagcgttg ccagtacagt attaaaaatg agctccaacg
     1981 gaagttgggt actttcatat aagtcgtcaa tgagatacgc aagctcacta ggaaataatc
     2041 tgtaggttaa atcagctggg aagtggtggg atgtctgcaa ggattgcaca taatcgggat
     2101 tcattgtaat ggacctcctt cgcatactgt aatcaacgca aaacttactg ttcagttcag
     2161 aaatataccg acattgtgag tatgttcgct gacgtcgata ataaaatggc ttgtatccat
     2221 tcaccagtcc cgacgcattt atgatgctga caaaagcaaa acgacatgga ggtgcctcag
     2281 aagtctggca ccacagcaac aaacgatggg gcatgaaaca cagggaacat tgcgcccctg
     2341 agcgttgagg atcttccacc ttgtatcctg gttcttcgtt atcatgagca gcgaacagat
     2401 ttggcaaagc gtatgcagcc tggtgcccct tagggtttaa gcccatatgt aagcaaatgg
     2461 cgcatacgct gagccactca tctgaaaccc gaacagttgt ccgtactcca taacgcatta
     2521 gcaagaatgg gcaataatga gtctgccaaa tccactttgt gttgatacca atgtgtccaa
     2581 aacaacgctg gatattgccg ccagcaacac tattgaacgg ttttctgttg ccagtggtgc
     2641 tgacggcgtt gatttcatca tcgctgaact gaggcagcac gatatggcct ttgtgttgat
     2701 ggaaggccgc cataacctgt gcgtttcagc ccgaaggttt tgagatcgcc gtagttaacc
     2761 ccaggcaagc tcgtgacttt gcccgtgcga tgggatatct ggcgaagact gacccgcatg
     2821 atgcgcgttt tttttgccca gatggctgca gttattaatt accatccgga acgagaccgc
     2881 tttatccgga ctttgccgga tgcagaacgt caggttctta atgccatagt cgtttgcaga
     2941 caccagttga tcgcaatgcc agttactgag caaaacaggt tgtacccggc tcacccgcaa
     3001 agcaaaaaaa acattaatat catcaaagcg ctggaagatg aacacccaca tccggaacca
     3061 ttttaaagaa ccagctgagc acctgagcaa cattaaaggc atcggcgtaa tgactacggc
     3121 ggtgctggta gcctgtatgc gcaaactgct gaccatcctg aatgcgatac tcagaaagaa
     3181 caaagaaatg taatgaatcc tgccatcagg ttactccata attttgttgg tcaagacagt
     3241 taattacgtg acgtatgctc aggcaccgga tgccgtttta cagggctcac ctgccatcca
     3301 gtagacgccc cggcccacga ctgcaaccat aattacgttg accagaaccg tcatgctgct
     3361 tcgtctcata cttcacgaga taacaggctc tctccatggc tttctgcatc gcttccacat
     3421 caccacgctt gacccagagc acagcggatc caccagattt tttgtcagaa gctttccggg
     3481 tatcagaaca tccacccgac aaaggcgtat catcggaaga agggcgagca tcagatgatg
     3541 tcggtttacg gaaaggcgtc cgcctggaaa aatgaaccag tccattaccc tgccagggct
     3601 cgagatgcag agcactgcac catgccagct tgatcagcgt cgccagcgaa gaaggaacgg
     3661 tgaaatcccc tggcgagcac caggtatctt tgttgagcag taatatcacg tgataatgtt
     3721 tcctgccttt ttccttgcca aactcccgac accagaaata acgcagagtg gttgcacgca
     3781 cacgcttgtt ggcacgtcgt ttatgtcgct ggtaagcctg aatttttgct ttcagggatg
     3841 cgaaaaaacg ggagatcacc gcagagtccg gatcgggatc catgatggtg acaggcatat
     3901 actcagggaa acgtaaatcc acccggatta acgaaacggc acggtgttgc accagggcac
     3961 tgttgagaat actttcaaaa cgggataacc agtaagcatt gtgtattggt tgattcatga
     4021 tgagagatct ccattgattt ggattgactt cacatcatta atcaccgcgg taaaacttta
     4081 cgaactccca ccattgagca acacaaccta ctccggataa aaatggaatc ccctacaaac
     4141 gaaaagatgg taaaaaaaca caacaacagc aaatgaattt aaaaacggca tggacgacga
     4201 gaaaagtcag aagggaaacg gatcaacaga acgtgcaaac aaaacatcaa aaacgcaatc
     4261 agaacgattt ctggcgctgt gaatcaacaa caacaccaaa acctatagct taaccacaca
     4321 actcaatcac agagccgaat agcgcgttta aaacctgttt acagataacc ccaccccaag
     4381 aacaaaaacc actaacatca gaatcgattt tctcatcctg aaaatgttac gtttcactac
     4441 gcaccataaa aacgattaac catcgataac gtaaacctca tctgaatcaa aacaccagaa
     4501 ccaatcatca cgctttttct gaaaagagcc ttgccctgag aaaaaaacac cagggccagt
     4561 taatcatctc atcaatctca tcgctacaaa atcatccata aaaccattta actaaatcgt
     4621 cgtaagcact ccgtaaatca cgccaggtta ttcagtcgga tttgcatttt tacgtaaaga
     4681 tacttttctc cggttaacgg ccaaaacacg acaactcata aaagattaaa tgtaacatca
     4741 gtaaaatcga atagcatatt gactgcttaa acaggacagg gagttttgta gtgacgttaa
     4801 ctgattttag ctatcgtaaa aacagaacta acaaatttac taaactggat gtgtatcaga
     4861 caaagcaatg tgtataacaa tacttaatga aaacacttgt agaagtgttt atgaaaatac
     4921 gtgtatgtct ggtaaaacac ttagtagaaa atacgtatat aacacttatt gaaatgctta
     4981 gtaaaaatac gtgtatataa tatcataccg acattagcaa cattctcaga cactcatgca
     5041 gactgaatgt tgattacccg ttttcacatc aggcatggtt ctttactgac caaaaaacaa
     5101 actggtttac tcttgaatta ttttctctac ctcagaaaac atggtctcag gaatctgcta
     5161 tggcttcgta gtttctgttg ttcttttcat aaaagttata agtcattgtc ataatcatac
     5221 gataatcact ttatgttaat ttttactgtc acacgctact gactttttca gtcattgcca
     5281 gaaaggggct ctttttactt ccatttcacc actcaacaaa tgcaaaactt gtccagtaaa
     5341 gcggaccacc tcttgctcat aagcaacgaa tcgtctccag gaacagtcct caaacattgc
     5401 agaaacaaaa acaacaaaac cctcattatc actgatgttc ggaaaatatc cctgaacaaa
     5461 aaaacgaaaa caaaatgaga cgcattatca tgaatgtagt caaaaagacg ttatggtggc
     5521 tttcagttaa atggtactat ttctcataaa tccacttaac tttttttact gattcatccc
     5581 agagaaccac tttatcagta aaaaatgaat tatatagatt gacgagtttt tctgtttcag
     5641 ttattcccaa tgtagtatgt atcagttgta gatatacttt tatccagatt tctttaccct
     5701 ccctccactt atctataaaa cttcccagct tacttaatcg ttgatttata aaaaacgaat
     5761 caatatcatg aaaaaactca acaagaaaac agcacgatac aatctcaaca aaaaacaaat
     5821 tctctttaaa agcattataa aaccttatca acacataacg aaaaacacct cctgttaatc
     5881 tttcctgttc ataaaacaac aaaccaaaaa caggaggaaa tcttttaacc acaccacttt
     5941 taatccagta aggatgatga ttagtcttta ctggcaatga ataaaagaac atttcggttt
     6001 ctcttttccc accacaaaca gacaaaactg tcttataaag actttttgaa tcattatgtc
     6061 ctagtgcccc agacgaatta gcagagtatt ttctttttac tttttgcctt aatggagctg
     6121 gaagaataat aaaacgagat ggcagataac aatcgctttc agaagaccgg cattcgcaca
     6181 catgaagatt tatatttaca tccggacaat tcaaaaacca cacagaataa ttatcgcccc
     6241 gtttgcaata tgaaacccat gaataaattg cataaagttc agaaagcgca tctaactctg
     6301 cttttgactt aaatgtgatt ccgtttcttc gaattctcgt ttccagcgtt ctccatgccg
     6361 gagttttcag tatctcacga acttttttat taagttcagt cagcgatggc ccctgctcct
     6421 gtttatgttt ttttctgctg ttaatgattt ctctttcttt tactaatttt ttcttacttt
     6481 ttggtccttt tatctttctt ggcaatgctt catttaatct gtgaacagga ttttgctctc
     6541 ttcttttttt gagctcacaa aacctctcat aatgttttct gcacagtctt ttctccactg
     6601 ccatttttac tgagaaaacc tgattgttac aattttttct gacacatttg tttttacgtc
     6661 cggtcatcct ctcatctcct gaaaccgcag tatgtcatta gttcccgttc ggaaaaactc
     6721 acttcatact ccattacact ttaacaatca tgtccgtaat tcagacattc catcattttt
     6781 cacgtccata ttacctgaat gataaaccgg attagtgtga tccggttcag tgaaatcaac
     6841 aggataccgg tatgtcattc agccattctt ccctctctgc acaagtgaaa tcatatctga
     6901 cgtttcttcc tgaagaaata cgccagaaaa tccttgaaca tctccacggt gttattcatt
     6961 acgagcccgt gattggcatt atgggtaaat ccggcaccgg caaaagcagc ctgtgtaatg
     7021 ccatttttca gtcccgtatc tgcgccacgc atcccctgaa cggctgcacc cgccaggctc
     7081 atcgtcttac cctgcagctc ggtgaacgca gaatgacgct ggtcgatctg cccggcattg
     7141 gtgaaacacc gcagcatgat caggaatacc gggagcttta ccgtcagtta ctgccggaac
     7201 tggatctgat tatctggatc ctgcgggctg atgaacgtgc gtatgctgcc gatattgcca
     7261 tgcatcagtt tttactgaat gaaggtgcag atccctcgcg ttttctgttt gttctcagcc
     7321 atgccgatcg cgtatttcct gctgaagaat ggaacgacac agaaaaatgc ccgtcccgtc
     7381 agcaggaact ctcactggca acagtaacag cccgggtggc aaccctgttc ccttcatcat
     7441 ttccggtact ccccgttgcc gcacctgtcg gctggaacct tccggcattt gtgtcgctga
     7501 tgatccacgc actgccaccg caggcaacca gcgcggttta ttcacatatc cggggggaaa
     7561 accgctctga acaggcccgg aaacacgcac aacagacttt tggtgacgcc atcggaaaaa
     7621 gctttgacgc tgccattacc cggttcagct ttccagcctg gatattacag cttctgcgca
     7681 aaacccggga ccgcattatc cacctgctgg tcacactgtg ggatcacctg ttctgagcgc
     7741 cccggagtcc cccgtggcaa ccgtatacct ttacacgaca acggattacg gtaaccactg
     7801 ccgtataccg tctgtcatta tcaataacac gctctttaat ttcagaggtc agtcatgaat
     7861 accagaattc tttttcccct gctgttcact gtggcatcat tctccgcctc cgccggcaac
     7921 tgggctgtca aaaacggctg gtgtcagacc atgacggaag atggtcaggc gctggtaatg
     7981 ctgaaaaatg gcacgattgg tattaccggc ctgatgcagg gatgcccgaa tggtgtacag
     8041 acgctcctgg gcagccgtat cagtattaac ggtaacctga tccccacatc acaaatgtgt
     8101 aatcagcaga cgggattcag ggctgttgag gtggaaatcg gacaggcgcc ggaaatggtc
     8161 aaaaaagccg ttcactccat agcagagcgt gatgtgtccg ttttacaggc atttggtgta
     8221 cgaatggaat tcacccgcgg tgatatgctg aaggtctgtc cgaaatttgt cacatcactt
     8281 gccggttttt ccccgaaaca gacgaccact attaataaag actccgtact gcaggctgcc
     8341 cgacaggcat acgcccgtaa atacgacgag gaaacaacag aaaccgctga ttttggctct
     8401 tacgaagtaa aaggcaataa ggttgagttt gaagtgttca atcctgaaga ccgtgcgtac
     8461 gacaaagtga tcgtcacggt tggtgctgac ggtaatgcca ccggagccag cgttgaattt
     8521 atcggaaaat agccgatatg tcggactgtc accctgtttt attgcccgaa ggccctttct
     8581 cacgcgaaca ggcgatggct gtcacaacag cttaccgcaa tgtgcttatt gaagatgacc
     8641 agggaacgca tttccggctg gttattcgca atgctgaagg gcagctacgc tggcggtgct
     8701 ggaattttga acctgatgcc ggaaaacagc taaatccgta tctcgccagt gagggaattc
     8761 tcaggcaata aacgtcttca tttcatccat caggccgcgt cttctccggg agacgcggcc
     8821 ttttcgttta taccgctaac tcattcataa ggaacaaagt atgcaattag ccagtcgttt
     8881 tggtcatgta aatcagatcc gtcgggagcg cccactgaca cgcgaagaac tgatgtacca
     8941 cgtcccgagt atttttggag aagaccggca cacctcccgc agtgaacagt atgcgtacat
     9001 tcccaccatc accgtcctgg aaaatctgca gcgggaaggc tttcagccat tcttcgcctg
     9061 ccagacccgt gtgcgcgacc agagccgtcg ggggtatacc aaacacatgc tgcgtctgcg
     9121 ccgtgacggg gagataaacg gacaacatgt ccctgaaatc atcctgctga actcccatga
     9181 cggtacctcc agctaccaga tgctgccggg ttacttcagg ttcgtctgcc agaacggctg
     9241 cgtctgtggt cagtctctgg gggaagtgcg tgttccgcac cggggaaatg tggtggacag
     9301 agtcattgaa ggggcttacg aggtggtggg cgtgtttgac cggattgagg aaaagcgtga
     9361 tgccatgcag tcgctggtcc tgccgccacc ggcacgccag gcgctggcac aggcggcact
     9421 gacttaccgt tatggtgaca aacatcagcc agtcaccact gccgacattc tgacgccacg
     9481 acgccgggag gattacggta aggacctgtg gagtgcttat cagaccatcc aggagaatat
     9541 gctgaaaggc gggatttccg gccgcagtgc aaaaggaaaa cgtatccata cccgggccat
     9601 tcacaacatc gacaccgaca ttaagctcaa tcgcgcattg tgggtgatgg cggaaacgct
     9661 gctggagaac ctgcgctgat gccgtttacc tgaatcacgt ctgtccctgc ccccgagcct
     9721 tcttcattca tttgcttttt ataaggaata atatcatgac aacctcttcg cataattcca
     9781 ccacaccttc tgtttccgtg accgctgcat cagggaataa cccgcctcag ttggttgcca
     9841 ctcccgtccc tgatgaacag cgcatcagct tctggccgca gcattttggt ctcattccac
     9901 aatgggtgac cctggagccc cgtgtcttcg gctggatgga ccgtctgtgt gaagactact
     9961 gcggtggtat ctggaatctg tacaccctga acaacggtgg cgcatttatg gcacccgaac
    10021 cggatgacga tgatgacgaa acatgggtgc tgtttaacgt catgaacggt aaccgcgctg
    10081 aaatgagccc ggaagccgcc ggcattgccg cctgtctgat ggcgtacagt catcacgcct
    10141 gtcgtacgga gtgttacgcc atgacggtcc actattaccg gctgcgggat tacgccctgc
    10201 agcatccgga atgcagcgcc attatgcgca tcatcgactg aatggagaga agcacaatgc
    10261 aacagctttc ctttctgccc ggagagatga cgcccggcga gcgcagcctc attctgcggg
    10321 ccctgaaaac cctggaccgc catcttcatg aacccggtgt ggccttcacc tccacgcatg
    10381 cggcacggga atggctgatt ctgaacatgg cgggactgga gcgggaagag ttccgggtac
    10441 tgtatctgaa caaccagaat cagctgattg ccggtgaaac cctcttcacc ggcaccatca
    10501 accgcacgga agtccatccc cgggaagtga ttaaacgtgc cctgtaccac aatgccgctg
    10561 ccgtgatact ggcacacaac cacccgtccg gtgaagtcac tcccagtaag gcagatcgcc
    10621 ttatcacgga acgcctggta caggcactgg ccctggtgga tatccgggta ccggaccatc
    10681 tgatagttgg tggcaaccag gttttctcct ttgcggaaca cggtctgctt taacccgtca
    10741 ccgccacaac catatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcccac
    10801 tatgaaaatc atcacccgtg gcgaagccat gcgtattcac cgtcagcatc cggcatcccg
    10861 tctttttccg ttctgtaccg gcaaataccg ctggcacggc agtgctgaag cgtataccgg
    10921 tcgtgaagtg caggatattc ccggtgtgct ggccgtgttt gctgaacgcc gtaaggacag
    10981 ttttggcccg tatgtccggc tgatgagcgt caccctgaac tgaatcagga cgggcattca
    11041 gatgagcaga attatcacca ccaccgtgta tacactgaat gagctatcct ccaccgcacg
    11101 ggaaaaagcc cgcgacgagt accgtcagca tcatgctgac agcaactggt atgaaaacgt
    11161 gtatgaagac ttcagggagg tctgtgacat tttcgggatt gacctccgcc agcgtgttat
    11221 ccggctcagc aacggcaggt ttatggaaga gccgtgcatc tggttcagcg gattctgcag
    11281 ccagggggac ggagcctgtt ttgaagggct ctggcactgg cagccagcgg caccccggaa
    11341 aatcagggag tatgccccgc aggaccgtga acttcatcgt atcgcggatg ccctgcaggc
    11401 ggtacagaag cgtaacttct ggcagttaca ggctgaaatc agtcaccggg gacgttactg
    11461 ccatccgtac agcatggata tcaccgtcac ccggaacagt ccgactggtc aggtaatgac
    11521 aaccgatgcg gaagccgcag tcagcgaagt cctgcgcgac ctggccttct ggttgtaccg
    11581 tcagctggaa aacgagtacg actggctgac ctcagacgcc gctgtggatg aggccctgct
    11641 catcaacgaa tacaccttca ccgaagccgg tctgcgcgcc ggctgactcg tcatcgttaa
    11701 actatcattc accgattttc tgtaaggatt ttatcgtgtc agactcactc catgaaacaa
    11761 attatcccga cgataacaac gaccgcccct ggtgggggct accctgcacc gtgacgccct
    11821 gttttggggc acgtctggtg caggagggta accggttgca ttaccttgcc gaccgcgccg
    11881 gtatcagagg ccggttcagc gacgcggatg cgtaccatct ggaccaggcc tttccgctgc
    11941 tgatgaaaca actggaactc atgctcacca gcggtgaact cagcccccgc catcagcata
    12001 ccgtcacgct gtatgccaga gggctgacct gcgaagccga caccctcggc agctgtggtt
    12061 acgtttatct ggctgtttat ccaacatccg aaacgaaaac gtaaaccacc ggaataatcg
    12121 tctgctccgg cccggtgcgc tacagcacca ctctcctcaa aatttaatct ctcatatcag
    12181 gaaaatcttc agtatgaaaa cattacccga cacgcatgta cgggaggcat cgcgctgccc
    12241 gtctcccgtc accatctggc agatactgct gtcccgactg ctggaccagc attacggcct
    12301 cacactgaat gacacaccgt ttgccgatga acgtgtgatt gagcagcata ttgaggcagg
    12361 catttcactg tgcgatgcgg tgaactttct cgttgaaaaa tacgcactgg tacgtaccga
    12421 ccagccggga ttcagcgcct gtacccgttc tcagttaata aacagtattg atatcctccg
    12481 ggcccgccgg gcaaccggcc tgatggcccg cgacaattac agaacggtaa ataacattac
    12541 cctgggtaaa catccggggg cgaaacaatg aaactggccc tgacgctgga agccgacagc
    12601 attaacgtgc aggcactgaa catggggcgc attgtcgttg acgtcgatgg tgttactctc
    12661 gctgaactga ttaacgtggt ctgcgataac ggttactccc ttcgtgttgt tgatgaatct
    12721 gaccggacat cagcagagcg cacgccacca tctgcggccc ttaccggcat acgctgcagt
    12781 accgcgcata tcacggcaaa ggacaacgcc tggctgtact cgctgtcaca tcagacgaat
    12841 gacttcggcg aatcagaatg gattcatttt accggtagcg gatatctgtt acgtaccgat
    12901 gcgtggtcgt acccggttct gcgacttaaa cgcctgggcc tgtcaaaaac gtttcgtcgt
    12961 ctgatcgtca cgctcattcg ccattatggc gtcagtctca ttcatctgga tgcctgcgct
    13021 gaatgtctgc cgggtctgcc cactttcgac tggtaatcag ggaaattcac aacatgaaat
    13081 cattaacgac agaaacggcg ctggatattc tgattgcgtg gctgcaggac aatattgact
    13141 gtgaatccgg gattatcttc gacaacgatg aggatagaac ggattcagca gcactgttgc
    13201 cctgcattga acaggccagg gaagatatcc gtaccctgcg ccaactacag cttctgcaac
    13261 agaaccggtg agtcttactc accatacttc attcccctga cgccagcctg aatacagctg
    13321 gcgttttcgt ttataaacca aaaggaaaaa cgatgatgtc agatatcaca gcctcccgtc
    13381 cggaagtggt caccgggcat acggacgtta tttgctcaac cagtatccgc cacattctgg
    13441 ctgtacgaaa gagtacactg ctgcaaatcg acacacttat ccggcaactg gctgaaatat
    13501 cagcaatgac agaaagtatt ggcggtaaaa ccgcccggga ctgggccatg aaacaggatt
    13561 ttcgctgcgg ttgctggctg atggagaaac cggaaaccgc aatgaaagcc atcacccgga
    13621 atctcgatcg cgaactctgg cgtgacctga tgcaacgttc agggatgctg tcattaatgg
    13681 atgcacaagc ccgtgataca tggtaccggt cactggagta cgataatttt ccggaaatca
    13741 gcgaagcaaa tatcctgagc acgtttgaac aactgcacca gaacaaagat gaagtgtttg
    13801 agcgaggggt gctcaacgtt ttcagaggac tgagctggaa ttacaaaacc aattcaccct
    13861 gcaaatttgg cagtaaaatt atcgtcaaca atctggtgag gtgggaccgg tgggggtttc
    13921 atcttatcac cgggcaacag gctgatcgac ttgccgatct ggaaagaatg ctacacctgt
    13981 tcagcggcaa accgatcccc gacaaccggg aaaacatcac tattcgtctg gatgatcaca
    14041 tccagtctgt tcagggtaaa gaggactatg aagatgagat gttcagcatc agatacttta
    14101 agaagggctc cgcgcacatc acgttcagga aaccagagct ggttgacaga ctgaatgaca
    14161 ttattgcgaa atactatcca aatgtattga ccgaaaggta atgtgaccct gccccgctat
    14221 taaacatgcc gtattactgc tatcattctt aatatttttc ggaggaagca tgactacaaa
    14281 agaccgtatt cagtcccgac tgaaccgttc aaagcgttat gtgttcaccc gggatgactt
    14341 cagggatatc gctggctatg atcaggttgg cagagctctt cgtacgctgg taaacgaagg
    14401 taagttgatg aaagtgggct atggtgttta caccaaagcc cgtcgcaacg ttattacagg
    14461 aaaaatcatg cctgcagcac ctggtggctc tgccgctgta atcaccgaag ccctggatcg
    14521 tttgaatatc agatacagct tgatcggcgc aacagaatgc tacaataaag gtcagtccac
    14581 acagattccg gtatctccgg agttcaaaac acctcctcgc tttaaaagag tgttagttgt
    14641 agggaaaagt aaaatcaatg cctgaattaa catcattatt tcctgacgtt gccaacgcac
    14701 tggatattga atctgtcgcc atcgtcgaaa aagattattt tgtggttaat cttttaaagt
    14761 tactaaaaga aataaaacca gaaacacata ctctcgtgtt tgcaggaggt accgcattgt
    14821 caaaagctgg tatctcattg aacagaatgt cagaagatat tgatataaag ctcgtgccaa
    14881 cagagaactt tatgcaaaat ggcagaaaca ggaaaagaaa aattcgcaag gaaatcgtgc
    14941 aaataatcac ggatgttctt cataattctg acattttcag ccttgataat gaaaacgccc
    15001 gtataacacg tgatgagtat cgttataacg agatccccgt acgttatccc cagacatttg
    15061 cccaggcccc ctgtcttcgt ccatttatta agctggagtt gatggaaacc actctgctgg
    15121 aacacccgga aagcagagat atttactcac tcgtcacgga gttaacgggt aatggcacac
    15181 ctgtaacagc attcccctgt gccaccattt tatccactca ggctgaaaag ctcatatcca
    15241 tgatgcgaag aacagccata cttcttcgca atcccgaaca acaggatgat gagtccctgg
    15301 tacgccacat ttacgacaat tattgcattg tcagggaaaa aggtgtgaat gttccagtac
    15361 tgaaaaattt tgttcagata tgtattcaac aggacattga gcgctatggt aaccagcacc
    15421 cacaatttcg taactcacca gttgatgaac ttaagaaagg gctggatgca ctccgggatg
    15481 atcctgtaca caaattgcgt tttcaacgat ttgttaaacc gatggtattt ggtaatacac
    15541 agattgactg ggaagaagcc tactcctgtt tcagacaaac agcgttgagt gttctgacca
    15601 actaacaaac gcgttaagta cgattccgcg catcagcaca tcgaaccaga caaaataatt
    15661 acagccaagc acaaaaaaga gggattatcg cgtcaaaaga attcgaattg cacatggggg
    15721 cctcattggt ggcctcaaaa ataacaaaca caaaacaatc aatataaaac aacttgttat
    15781 acaaccagtt tcattccgag tccgggcacc aaattcatat caacggacct ccacggaggt
    15841 ccgtttttcg tttcagaaca ccatgattta agtgttcttc ctccaaatca attctaccga
    15901 actcaaccaa attctcccca catcaacctc attgtgtggg tataattgcg ggtatacccc
    15961 agttcgacag aatttgtacc ctctttaacg cattcagaag gctgaactat ggcactgact
    16021 gacgcaaaaa tccgggctgc aaagcccact gacaaggctt ataaactcac tgacggggcc
    16081 ggcatgttcc tgctggttca tcctaatggt tcccgttact ggcgtctccg ttatcgtatt
    16141 ctgggtaagg agaagactct ggcacttggt gtgtatccag aagtttctct ctccgaagct
    16201 cgtacaaaac gggatgaggc ccgaaaactg atttcagagg ggattgaccc ttgcgaacag
    16261 aaaagagtta aaaaagttgt ccctgattta cagctctctt ttgaacatat tgcacgacgc
    16321 tggcatgcca gtaataaaca atgggcacaa tcacacagcg ataaagtact caaaagcctc
    16381 gagacacacg ttttcccctt tatcggcaac cgggatatca caacactcaa taccccggac
    16441 ctgcttatcc ctgttcgtgc tgcagaagca aaacaaattt atgaaatcgc cagtcgtctg
    16501 cagcaaagaa tatctgctgt aatgcgttac gccgtacagt ctggcatcat cagatataac
    16561 ccggctctgg atatggctgg tgcattgacc acggtaaaac gccagcatcg ccctgctctg
    16621 gagctttcac gcctgcctga acttctgtcg cgtattgaca gttataaagg ccagcctgtc
    16681 acccgactgg cagttatgct gaatttactg gtttttattc gttccagtga actcagatat
    16741 gcccgctggt cggaaattga tattgacaat tccatgtgga ctattccagc cgaacgcgaa
    16801 cctctgccag gcgtaaaatt ctcacaccgg ggatccaaga tgcgaacacc acatcttgtg
    16861 ccactcagca aacaggttgt agccatactg gcagaacttc agacatgggc tggtgaaaat
    16921 ggtctgatct ttacgggagc acatgaccca cgtaaaccaa tcagtgaaaa caccgtaaat
    16981 aaagccctga gggttatggg gtatgacaca acccaggatg tctgtggtca tggattccgg
    17041 gcgatggcat gtagtgcgtt aatagaatca ggtttgtggt cccgcgatgc agttgaacgt
    17101 cagatgagcc atcaggaacg taacggtgta cgtgctgctt acattcataa agcagaacat
    17161 ctggaagaac ggcgactgat gctacagtgg tgggcagatt ttctggatgc gaacagagaa
    17221 caatgtatca gcccgtttga atatgcaaag attaacaatc cattaaaata gtaatcatcc
    17281 cgggctgcca gcccgggaat tattttcaat acctttttct ttgagattaa tgaataaaca
    17341 aggcaattga cagaaccact tccattacgt catgtaatca aaatttttat agccgacatc
    17401 agggatatct gaacctcctg gtcgcgggta attcctcttc aggtattttc gggtgctcaa
    17461 ctctggttat tagcaaaata atcgatataa acagtggaaa attcatcgta cagaacatgg
    17521 tgctcaacat tactcttatt ttcccttgtc cggaagaatc acaggctgtg acttaaggcc
    17581 gtccgcagga actattttct ctccgttaaa aaccataaca gtttcattat cttcatttat
    17641 cagcgggttg aatggcggta tattttcagt acgaatacca gtcagccact gaaaaat
//
LOCUS       DAPHDX010000092        17659 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPHDX010000092 DAPHDX010000000
VERSION     DAPHDX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.093"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(314..1327)
                     /locus_tag="RH058_003098"
     CDS             complement(314..1327)
                     /locus_tag="RH058_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV3138654.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVASINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
     gene            1434..1730
                     /locus_tag="RH058_003099"
     CDS             1434..1730
                     /locus_tag="RH058_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3138655.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1864..2289)
                     /gene="arsC"
                     /locus_tag="RH058_003100"
     CDS             complement(1864..2289)
                     /gene="arsC"
                     /locus_tag="RH058_003100"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV3138656.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2302..3591)
                     /gene="arsB"
                     /locus_tag="RH058_003101"
     CDS             complement(2302..3591)
                     /gene="arsB"
                     /locus_tag="RH058_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV3138657.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3645..3998)
                     /gene="arsR"
                     /locus_tag="RH058_003102"
     CDS             complement(3645..3998)
                     /gene="arsR"
                     /locus_tag="RH058_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV3138658.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4738..4821
                     /locus_tag="RH058_003103"
     CDS             4738..4821
                     /locus_tag="RH058_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV3138659.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4875..6227)
                     /gene="gorA"
                     /locus_tag="RH058_003104"
     CDS             complement(4875..6227)
                     /gene="gorA"
                     /locus_tag="RH058_003104"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV3138660.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6299..7141)
                     /gene="rlmJ"
                     /locus_tag="RH058_003105"
     CDS             complement(6299..7141)
                     /gene="rlmJ"
                     /locus_tag="RH058_003105"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV3138661.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7344..9386
                     /gene="prlC"
                     /locus_tag="RH058_003106"
     CDS             7344..9386
                     /gene="prlC"
                     /locus_tag="RH058_003106"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV3138662.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9392..10144
                     /gene="rsmJ"
                     /locus_tag="RH058_003107"
     CDS             9392..10144
                     /gene="rsmJ"
                     /locus_tag="RH058_003107"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV3138663.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10193..11662)
                     /gene="dtpB"
                     /locus_tag="RH058_003108"
     CDS             complement(10193..11662)
                     /gene="dtpB"
                     /locus_tag="RH058_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV3138664.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLTPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11980..12414)
                     /gene="uspA"
                     /locus_tag="RH058_003109"
     CDS             complement(11980..12414)
                     /gene="uspA"
                     /locus_tag="RH058_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV3138665.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12805..13140
                     /gene="uspB"
                     /locus_tag="RH058_003110"
     CDS             12805..13140
                     /gene="uspB"
                     /locus_tag="RH058_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV3138666.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13211..14710)
                     /gene="pitA"
                     /locus_tag="RH058_003111"
     CDS             complement(13211..14710)
                     /gene="pitA"
                     /locus_tag="RH058_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV3138667.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14942..16144
                     /locus_tag="RH058_003112"
     CDS             14942..16144
                     /locus_tag="RH058_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV3138668.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16460..17512)
                     /locus_tag="RH058_003113"
     CDS             complement(16460..17512)
                     /locus_tag="RH058_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV3138669.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4585 a         4444 c         4544 g         4086 t
ORIGIN      
        1 tttgtgatgt tttagatgtg taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg
       61 tattagcgac tgaaatctgc cttttaaaaa ggaggagggt actgagacaa agagtcattg
      121 cttctccgat aaaatgtcag agaagcatga ttacgtggtt attgtctgtt gatgacttgt
      181 tgaccgagcc gtcatcattc tcaatgcatt agttaaatca tttgtgattt ttaaggatta
      241 tcctgtctat gattttctac ttagaaggat atggcactaa ctatgcatct tatagtttac
      301 tgctggcatc tatttaaaat agcaattgag tcaaatagcc catcaagata gccataccta
      361 atataaccgt caggaatgca gctatcatcg gcattctgaa cattgattta agtaatatca
      421 cttccgtcag gcttgcgccg gcactgccga tgattaatgc cattaatgct cccagaccca
      481 ttccttttgt catcagaaca gatgccagag ggataaccgc ctcagcccgg atatacagcg
      541 gaataccgac aacggcgctt aatgggatgg cgaagggatt atctgctcct gcatgagcgg
      601 caatccacgc tgagggaata aagccataaa taaaagagcc tattaatacc cctaacaaaa
      661 gatatggcag tacatcttta aactgttgca acgcatcttt ggtggctagt tttatgggat
      721 taatacttgc cactgctttg gtattgcaac agttaacgac gggtttctca atggctttag
      781 ccgttggact acagcaactc acttctattg gcgtatatgt cgtccccggc aaagtttttg
      841 ccgacgcttt ggctggagca caacaatttg ctgatgagct tttactggca atgatatgac
      901 gttcaaaccc cagagaatcc aggataatac tggcaagaac ggagacgccg gtggcgataa
      961 tcgcgtacaa caaggtgact ttccaaccaa aggtcaccca cattagtccg acgataatgg
     1021 gattaagtaa tggtgaaaca aataaaaaag tgagagtcgg accaaacccg gctttcgctg
     1081 acaacaatcc acgtagcatg ggtattgtcg agcaactaca gaacggggta acggctccca
     1141 acagagcagc caggagatag cctctccctt ttctggcccc catcatctgc tggattttat
     1201 ggtctggcac cttttgtctt atcaggctga caccggcact aatcactata aacagcaacg
     1261 aaagctcgac ggcgagaaac acaaacatct ctgcggcatc ttgcagcatc gcaagccaac
     1321 tactcatacg taacctcatt ggataaatct atatttccag aataatcgaa atgaaattca
     1381 gatcaagata tttctggtat tatcgaaata tcaaccagac agaggggtgt catatgcagt
     1441 tggaagaagt cgctaaagcg ctgaaagagc taggtcaccc gacccgtttg ttcatattta
     1501 aacatcttgt caaagccggg gaacaagggt tgccggtcgg tgagttgcaa aagcagcttg
     1561 ggatacctgg ctccacactc agccaccata tatcagcact ggtatcggta ggactggtca
     1621 aacaaaaccg tgagagtcgg acgctgatgt gtgtttcgca atacgcaatg ctggaagcga
     1681 taattgaatt cttacgcgag gagtgctgcg taaatagcaa aattgcctga tgcgctacgc
     1741 tctacatatt tcatgccata tattgaattt gcacggtttt gtaggtcgga taagacgcgt
     1801 tagcgtcgca tccgacataa acaaagcgca ctttaccaac tatgggcagt ggatatcgcc
     1861 gctttatttc aaccgcttac ccgcttcatc aaccactttc tcgccatctt ccttggagaa
     1921 tgcgcctttt tgcgcatctg gcagaatttc cagcaccact tctgaagggc ggcacaggcg
     1981 agttcccagc ggcgtcacca caatcgggcg attaatcaga atcgggtgct gaagcataaa
     2041 gtcgattaac cgatcgtcag taaatttatc ttctgcaagg cccagttcct catacggctc
     2101 gacgttttta cgcaacagcg cgcgtacgga aatccccata tcggcaatga gtttgaccag
     2161 ctcatcgcgc gttggcggtg tttccagata atagataatg gtcggttctg tgccgctgtt
     2221 gcggatcatc tccagcgtat tacgcgacgt gccgcaggcc gggttgtgat aaatggtaat
     2281 gttgctcata tcagtatctc attacaaagt gaaagagaga cgtagcgcca gcgcagccag
     2341 cgtcacaaac agcacaggca gggtcatgat aatccctgta cggaagtaat atccccagct
     2401 gatagtcata ttcttctgcg aaagtacgtg cagccagagt agcgtagcca ggctaccaat
     2461 tggggtaatt ttcggtccca aatcgcagcc aatcacattg gcataaacca tcgcttcttt
     2521 gataacgcca gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat
     2581 attgttcata atagaagaga ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca
     2641 caggccgtta tccgccagca cgttgagtac gccagaaaga tattccgtta atccggcatt
     2701 gcgcaggcca taaaccacca gatacatgcc gagcgagaag atgacaatct gccagggggc
     2761 accgcgcagg actttacccg tattaatcgc atgaccgcgt ttagcgacga caaataatat
     2821 cagcgcgccc acagctgcaa tggcgctcac cggaatgccg agcggttcca ggacgaaaaa
     2881 tcccaccagc agaagcagta aaacaaccca gccagctttg aacgtagcag gatctttgat
     2941 cgcttctgcg ggagatttca gcagcgccat atcgtagttc tgcggaatat ctttgcgaaa
     3001 atagagatgt aacatcacca gcgtggcaac aatcgcggcg atatccaccg gcaccatcac
     3061 cgaggcgtat tcgcgaaagc cgaggccaaa gaaatcagcg gaaacgatat tcaccaggtt
     3121 ggagacaata agcggcaggc tggcggtatc ggcaatgaat ccggccgcca tcacgaacgc
     3181 cagcgtagtg cctttactga accctaaagc cagcagcatg gcgatgacaa tcggtgtcaa
     3241 aataagcgcc gcgccatcat tggcaaacag ggcggcaacg gcagcaccga gcaggacaat
     3301 ccaggtaaac agcaagcgac cacgaccatt accccagcgt gagacgtgca gcgccgccca
     3361 ttcaaaaaag ccggactcat ccagcagcag gctgatgata atgacggcga taaacgcagc
     3421 cgtcgcgttc cagacgatat tccacaccac cggaatatca cccggatgga ccacgcccgt
     3481 aactaacgcc agtactgcgc cgagcgttgc actccagccg atgcctaaac ctttcggctg
     3541 ccagataacc aatacgatgg tcaggacaaa gatagcgcct gccagtaaca taatgcctcc
     3601 cggataaaac acatctgaaa attcatatgt gtttagctaa atttttaact gcaaatgttc
     3661 ttactgtccc cggaacagtt ttgtcgagcc aggttgcgga caatcgcctg aaccttttcc
     3721 tgttcacatc gccaggcctc atcaataatt ttcgccgccc atgctggaat atgcggtgat
     3781 aagcggtaat gaacccactt accttgcttg cggtccagca atagcccgct ttcacgcagc
     3841 aatgccaggt ggcgggagat cttgggctgc gactggtcga gagcagtgca gagatcgcag
     3901 acgcataact ctcccagttc gctgagcagt aaaacgatgc ccagacgggt ttcatcagca
     3961 agaattttga acaattggat gggtaacaga aatgacatat tgcgctcctg attgttgcag
     4021 gtagtgtctc tcttcgaagc ggataagtca aaaacatata tgacttaacg aatgtgtaag
     4081 tgcagaggaa ggtaataggt gtgaattttg agttggctat tcatttgaaa agaggtctga
     4141 atcaggacgt aaaaaaaccg actttgcgtc ggttttttac tttccagccc tgagttggtg
     4201 gctctggctg gagtgagaga gttcttatct aacagctcaa tactattagc cagtggctaa
     4261 cattgcaaga attagcgatt tcttcagctg gcggaggggg cacttcggta tatatcgcaa
     4321 tgacgattta tatactttca ctgggtcatc gtcatattaa gccttgtccg aatccggatg
     4381 taaaaaaacc gactttgcgt cggtttttta ctttccagcc ctgagttggt ggcactggct
     4441 ggagtgagaa tgccattatc taacgcatca atgctaatac catactgaaa ctattgcaag
     4501 gacatgctgg ttttataacc tgaatgtact gtatgattat ccagttagct ctgaggcatt
     4561 ttcactctgg taatgcgcat aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc
     4621 gttaactgat gctctggccg gagtgagaat gccattatct aacaataggg catgcgccgt
     4681 gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag aaaacgggtg
     4741 attgataaag caatcatcgt tcttggggcg ttaattgcgc tgctggaact gatccgcttt
     4801 ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc acactactct
     4861 tagcccttta acatttaacg cattgtcacg aactcttctg ccgccgttgg gtgaatggcg
     4921 acggtattgt cgaagtcttt tttggttgca cccatcttca gcgccaccgc gaagccctgc
     4981 aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc ttccgatcca
     5041 acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt atacatcgcg
     5101 gtgaaagagg atttatacac tttcacctga tcgtcgccat actgctcgcg cgcctgcggt
     5161 tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg aatgttgctg
     5221 taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg gcgacccgct
     5281 gcaactgcca ccggtgtcag ctccactgca cccgtgttat cgcccaccgc gtaaatacct
     5341 tcaatattgg tgttttgata tttatcgacg acgatatagc ctttttcgtt agttttaacg
     5401 ccagcggctt ccaggttgat gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg
     5461 caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc atcggcattt
     5521 ttcactaccg ctttcgggat ggcgttggtg tgcagctgcg ggccttcggc gttcatcact
     5581 tcgaccagcg tttcggaaat catcgggtcg aagctgcgca gcggcgcatg tttacgcaca
     5641 aacagatgcg ttttcgcgcc gaggccgtta atcacgcccg ccagctcaac ggcgatgtaa
     5701 cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa gccatcagaa
     5761 tcaataccgt attccacgcc cggaatatcc gggtggctcg gacgaccgcc tgtggcgatc
     5821 agaatatgat cggccgtgat ggtttcgccg tttacctcca gcgttttggc atcaacgaag
     5881 cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc ataggaagta
     5941 tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt gaatttattg
     6001 atagtggtat caaaaccata atccgggccg tacatatgga tcgcttcacg gatttgcgcc
     6061 gcgtgccaca tcactttttt cggcacacag ccaacattta cgcaggtgcc gcccagctct
     6121 ttggcttcaa tcagcgcaca tttctggccg tacatagccg cgcggttgat ggaggcgata
     6181 ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac aaagtgtcct
     6241 tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt
     6301 actccggcac gatccagctt acggtggcgt gcccggtgcc tgccggaacc agtttgctgt
     6361 gcagccacgg cagcacgtta ttcatctgct gttccagttt ccacggcggg ttaatcacaa
     6421 tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagtaccgcc agttcaattt
     6481 gcagaatttt gcgaataccg gtcgcttcca gatcgtggat catgcgctta atttgctgac
     6541 gcagcaccac cggataccac agtgcgtaag taccagtggc gaaacgtttg taaccttctg
     6601 ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg tcgataagga
     6661 ttaaaccacg gcgggaaacc ggcggcagtt tggccttaag ctgctggaaa ccatcggctt
     6721 tttcgacgcg cgcacggctg tctttctgaa attcagaacg cagcaacggg taatcgctcg
     6781 ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga gcaatcaatg
     6841 gcgaaccggg gtagtaacgc aactgaccgc tacggttgaa gtgttttacc acattgatgt
     6901 acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgatg ccttcgagat
     6961 attcgccggt acgctcggca tgttcgctgc ctaactgata acgcccggcc cctgcgtggg
     7021 tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc aggctctgaa
     7081 cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga taactgagca
     7141 tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt cggggcgcac aaaagcgtat
     7201 caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct cattgaaatt
     7261 cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc gcttccttaa
     7321 cctctttaac caggactgcg cgaatgacga atccgttact gactcccttt gaattgcctc
     7381 cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggcg ctgaacgact
     7441 gccgcgaaaa tgtggagcgc gtagtagcgc aaggagcacc gtacacctgg gaaaatctct
     7501 gccagccgtt ggcggaagtg gacgatgtgc tggggcgtat cttctccccg gtcagccacc
     7561 tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc
     7621 tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg tatcgcgacc
     7681 tgcgcgatgg cgatcattac gccacgctga acacggctca gaaaaaagcg gttgataacg
     7741 cactgcgcga cttcgaactc tctggcatcg gtctgccaat agagaaacaa caacgctacg
     7801 gtgagattgc cactcgtctt tccgagctgg gcaaccagta cagcaacaac gtcctcgatg
     7861 cgacgatggg ctggaccaaa ctcgttaccg acgaagcgga gctggcgggg atgcctgaaa
     7921 gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc tacctgctga
     7981 cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag gccttgcgtg
     8041 aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaac gccggtaaat
     8101 gggataacag caaggtgatg gaagagatcc tcgctctgcg tcacgaactg gcgcaactgc
     8161 tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa aacccgcagc
     8221 aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc
     8281 tggcacaact gcgcgccttt gccaaagccg aatttggcgt cgatgagttg cagccgtggg
     8341 atatcgcgta ctacagcgaa aaacaaaaac agcacctcta cagcatcagt gacgaacagc
     8401 tgcgtccgta cttcccggaa aacaaagcgg ttaacggttt gtttgaagtg gtgaaacgta
     8461 tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcacccg gatgtacgtt
     8521 tcttcgaact gtatgacgag aacaacgaac tgcgcggtag cttctacctc gacctgtatg
     8581 cccgtgaaaa caagcgcggc ggggcgtgga tggatgactg cgtaggccag atgcgcaaag
     8641 ctgacggttc tctgcaaaaa ccggtcgcgt atctgacctg taactttaac cgcccggtaa
     8701 atggtaaacc ggcgctgttt acccatgacg aagtgatcac cctgttccac gagttcggtc
     8761 atggtctgca tcatatgctg acccgcatcg aaaccgccgg tgtatccggg atcagtgggg
     8821 tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc tgggagcctg
     8881 aggcgctggc gtttatctct ggtcactacg aaaccggcga accgctgccg aaagagttgc
     8941 tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg cgccagctgg
     9001 agttcggcct gttcgatttc cgcctccatg ccgagttccg cccggatcag ggagcgaaaa
     9061 tcctcgaaac tctggcagaa atcaagaaac tggttgccgt agtaccgtct ccatcctggg
     9121 gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgccgca ggttactaca
     9181 gctacctgtg ggctgacgta ctggcggcag atgctttctc gcgctttgag gaagagggca
     9241 ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt ggcggttcag
     9301 aggagccgat ggatctgttc aaacgcttcc gtggtcgtga gccgcagctg gatgcgatgc
     9361 tggagcatta cgggatcaaa ggttagtttt tgtgcagatt tgcttaattg atgaaacagg
     9421 caccggagac ggtgccttat ctgttctggc ggcccgctgg gggctggagc acgatgaaga
     9481 caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc gtgatgagcc
     9541 aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt
     9601 cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg attatttgcc
     9661 ggatgtggtg gacgccaccg cagggctggg gcgcgatgcc tttgtgctgg cttcagtcgg
     9721 ttgccgcgtg cggatgctgg agcgtaatcc agtcgttgcc gccttgctcg acgacggcct
     9781 ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg caggagcggt tgcagttaat
     9841 tcacgcctcc agcctgacgg cgctgactga tattaccccg cgcccgcagg tggtttatct
     9901 cgacccgatg ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt
     9961 tcagtcgctg gtggggccgg atcttgatgc cgatggattg ctggagcctg cgcggctgct
    10021 ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg ccaccgctgg cgaatgtcgc
    10081 cacgccaaac gcggtagtca ctaaagggca taggtttgat atttatgccg gaacgccggt
    10141 gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat aattaatggc
    10201 tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc actactgcaa
    10261 cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacgggc aacgtctcaa
    10321 gcggatcggt aatgttgtcc ggcaccgcgg taaatgttgc cacatagccg cccagcaaga
    10381 acgcggcagc ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca
    10441 ccagggcagc aatcatcgcc agacccaggg cgctaataaa caattcaccc aggctctgga
    10501 acaagtaaac cagcacgata aaccacggcg atgtcagtcc ttgtgcatca gcaaaccaca
    10561 ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaatt
    10621 tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta
    10681 ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc gggttgatgg
    10741 aaaagccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gacgttggca
    10801 tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca
    10861 ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga
    10921 cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc agccatgcac
    10981 agacgaagat catcaccacg ctgccaagta acacgtacaa cagtttgctg aagctcattg
    11041 gccggaagtc gggttcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca
    11101 gtaatgcgat aattaacccc gccccgcaca ggttgtaggt gactgaataa ccgaatctat
    11161 cagcgatcac aggggtcagc gataacgcta tcaacgagcc gatattgatc gacatataga
    11221 acagggtgaa tgcgccatca agccgcggat ctttcggcgg atagcacttc gaaagcaagc
    11281 tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa
    11341 tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg
    11401 ctccgagaac aatggtgcgt ttggtcccca gcaggtggtc gccgacatag ccgccaatgg
    11461 aaatgaggcc atagaccagc gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg
    11521 agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga
    11581 atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc
    11641 ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga aaagtatgaa
    11701 cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg
    11761 gtgaatagat taatacacta ttacaacaga aaataaccag ctgatcacac taatgatgga
    11821 aaatatgatg gtgttaaatg ttgtattggc aggagatttt gattgcgtac gcagatcgta
    11881 ggccagataa ggggtttacg ctgcatcagg taacaaggcg cgccaaaaag caaaacgccc
    11941 gcatatgcgg gcgttcagga acacgtcgtg gggggatgat tattcttctt cgtcgcgcag
    12001 cggaacaatc agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg aagacatcag
    12061 tttgctccag aagtcctggt ggtgaccaca aaccaccaga tccatatcgt atttcttgat
    12121 tgcatcgacc agaacctggc ccaggtcgcc gctgccgctc agggtttcag tgattgggta
    12181 gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg
    12241 catatcaccc agattcacat caataagccc ggtgtatagg tcagagtagt ttacatctac
    12301 gtggatcaga gaaactttcg cattgtaggg gcgagccata gagactgctt tctctaccag
    12361 aactttgctt tccggggaga gatcgactgc gatgagaatg tgtttataag ccatagtgtt
    12421 actccttcca taaagttgtc gatgactggc cagctagcgt ttcttgtgcc gttacgtcac
    12481 ccgcgtcctg cgaactacgt gcgcggggct aacccagccg taaagattca gtgttaagac
    12541 catccttacc ttatagcgac ccggatgatc cgtcaatccg ccttgcttac ccgttagata
    12601 aacaaatttt cgacatgaat atattgatag tggttaacct tctggaaaaa aaacaacctg
    12661 atctcctaca ctatctatag agccgctcgg atgttggtca ccacaaaagc ggtttctcca
    12721 ggacctggtg ttaactgatt ggcagcgtat cgggagcggt agccccgggg tggaactccg
    12781 cgggcaggtc gccggggagg aggtatgata agcaccgtcg cattattttg ggctttatgt
    12841 gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac tacgcgcgtt gttagtggta
    12901 ctgcgtaact gcgatccatt gctctatcaa tatgttgatg gagggggctt ttttacctca
    12961 catggccaac ccaacaaaca ggtgcgtctc gtttggtata tctatgccca acgttatcgc
    13021 gatcatcacg atgatgaatt tattcgccgc tgtgagcggg tgcgtcggca gtttattctg
    13081 accagcgcat tgtgtggtct ggtggtggtc agcctgattg cattgatgat ttggcattaa
    13141 gcacttcagc aataataacg cataaaaaaa gcgggccagt aaactgaccc gctcgttttg
    13201 gtgtgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa agcagtaccg
    13261 cagccggaag ggtaaacacc caggccatca gaatgctggt cacggtttta cgctgtaagc
    13321 cgccaccatc taccaccatc gtccccgcga cagaagagga gagtacgtga gtagtggaaa
    13381 ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc tgggcagaca
    13441 tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc gttgccacac
    13501 ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg atgatccaca
    13561 ccggtgcata ctcgatggtg ctaagcatgt cagacttcag tttcttcaac aggcgctgat
    13621 cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa acgcacagca
    13681 taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt tccacatcgg
    13741 tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga tggcagtgga
    13801 actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca gcaccggtag
    13861 cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg atggcatcac
    13921 gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct gctggcgcga
    13981 cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc gcgccgtgcg
    14041 aaaacgccac gccgatagcg gaaaggatca gcgcgatacg cgtccagaac ggcggctttt
    14101 tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg cgtttcttgg
    14161 tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc aggccgacaa
    14221 taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca
    14281 ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca
    14341 gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg
    14401 ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca
    14461 gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac
    14521 ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc gcgcgggtat
    14581 agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca
    14641 gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca
    14701 aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact
    14761 ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatttc
    14821 actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt aatattgatg
    14881 ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag aggagcgcaa
    14941 cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt tctgttctgc
    15001 gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa aaccagggcg
    15061 caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg tcgaaccagg
    15121 cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcgcgtt ttacccagtg
    15181 ggatttcatt gatctggtca ataaacacgg catcgcctgg cacgagaaaa cgttaggaca
    15241 actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg agtgcgagaa
    15301 gggcaatgta accttcagat tgcgtagcga agtgctgagt gtggcgaagg atgaaacagg
    15361 cttcacgctt gaactgaacg gcatgactgt cggttgcgaa aagctggtca tcgcgaccgg
    15421 tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg ccgaacaatt
    15481 tggcctcaac gtgctgccga cccgcgcggg tctggtgcca ttcactctgc ataaaccgtt
    15541 gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta ccgctgaaaa
    15601 cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg gaccggcggt
    15661 gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc tgctaccgga
    15721 tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc agagcctgaa
    15781 aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc aactcgggca
    15841 aatcccggat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac tgattagcac
    15901 attgaccgac tggcgcgtac aacccaacgg cactgaaggc tatcgcactg ccgaagtgac
    15961 gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc gcaaagtgcc
    16021 tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg gctataactt
    16081 ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag cgaagtcgtc
    16141 ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag ctaattttga
    16201 tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct ccaggcttaa
    16261 tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt tataaaatat
    16321 gaacaggttg aaaaaaacag taactttgtt gtttttttat ccttaaccac tatgcattaa
    16381 tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt
    16441 tttatccggt acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg
    16501 agaacaacgt aaagcatacc gctcctaaac cgaccaaaac ccgcgaagga ataaaatatc
    16561 ccatgtcggt ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg
    16621 tgaagacggg aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg
    16681 ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa ataagaataa
    16741 tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta
    16801 ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa
    16861 acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg
    16921 cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta
    16981 acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc
    17041 aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg ctccccgctg
    17101 catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat acacaggcgg
    17161 caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc atcacatcat
    17221 tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa
    17281 tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa
    17341 atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca tgacccgcga
    17401 cgaaatactc gctttgccct gaactttcca gcacaaagcc acctaacgcg atgcaaatta
    17461 atcccataat caaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact
    17521 cctcaggtat ccctgctaat aatgaaatgt gcattaatga taatttcagc ctgttaatat
    17581 aacgacgaga tatcattaaa gacaaatgca tagcataact atagcggggt ttactgtgcc
    17641 gagtgccatt ttttaccgg
//
LOCUS       DAPHDX010000093        17632 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPHDX010000093 DAPHDX010000000
VERSION     DAPHDX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.094"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..1977
                     /gene="csrD"
                     /locus_tag="RH058_003114"
     CDS             37..1977
                     /gene="csrD"
                     /locus_tag="RH058_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV3138670.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            2282..3325
                     /gene="mreB"
                     /locus_tag="RH058_003115"
     CDS             2282..3325
                     /gene="mreB"
                     /locus_tag="RH058_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV3138671.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            3391..4494
                     /gene="mreC"
                     /locus_tag="RH058_003116"
     CDS             3391..4494
                     /gene="mreC"
                     /locus_tag="RH058_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV3138672.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            4494..4982
                     /gene="mreD"
                     /locus_tag="RH058_003117"
     CDS             4494..4982
                     /gene="mreD"
                     /locus_tag="RH058_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV3138673.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            4991..5584
                     /gene="yhdE"
                     /locus_tag="RH058_003118"
     CDS             4991..5584
                     /gene="yhdE"
                     /locus_tag="RH058_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV3138674.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            5574..7043
                     /gene="rng"
                     /locus_tag="RH058_003119"
     CDS             5574..7043
                     /gene="rng"
                     /locus_tag="RH058_003119"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV3138675.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            7111..10911
                     /gene="yhdP"
                     /locus_tag="RH058_003120"
     CDS             7111..10911
                     /gene="yhdP"
                     /locus_tag="RH058_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV3138676.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            11067..12512
                     /gene="tldD"
                     /locus_tag="RH058_003121"
     CDS             11067..12512
                     /gene="tldD"
                     /locus_tag="RH058_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV3138677.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(12640..13569)
                     /gene="aaeR"
                     /locus_tag="RH058_003122"
     CDS             complement(12640..13569)
                     /gene="aaeR"
                     /locus_tag="RH058_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV3138678.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            13752..13955
                     /locus_tag="RH058_003123"
     CDS             13752..13955
                     /locus_tag="RH058_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV3138679.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            13963..14895
                     /gene="aaeA"
                     /locus_tag="RH058_003124"
     CDS             13963..14895
                     /gene="aaeA"
                     /locus_tag="RH058_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV3138680.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            14901..16868
                     /gene="aaeB"
                     /locus_tag="RH058_003125"
     CDS             14901..16868
                     /gene="aaeB"
                     /locus_tag="RH058_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV3138681.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            16960..17232
                     /locus_tag="RH058_003126"
     CDS             16960..17232
                     /locus_tag="RH058_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV3138682.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(17288..17551)
                     /gene="yhcN"
                     /locus_tag="RH058_003127"
     CDS             complement(17288..17551)
                     /gene="yhcN"
                     /locus_tag="RH058_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV3138683.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         4109 a         4320 c         4907 g         4296 t
ORIGIN      
        1 tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac gaaattttcg
       61 gcctttgtta cgctgctcac cgggttaaca atttttgtga ctttgctggg ctgttcgcta
      121 agtttctaca acgcaattca gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg
      181 attgataccc accttgtgtc gaatgacttc agcacattaa ggccacaaat taccgaatta
      241 atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg gtgataaaca ggtttatacc
      301 ctggccagaa atggtagtta tcgtccggtt ggctccagcg atctgtttcg cgaactgagt
      361 gttccgttga taaagcatcc ggggatgtcg ctgcgtctgg tttatcagga tccgatgggc
      421 aactatttcc attctttaat gactaccgcg ccgctcacgg gggcgattgg ctttatcatt
      481 gttatgctct ttctggcggt acgctggtta caacggcaac ttgccgggca agaattgctg
      541 gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt gggaaccatc
      601 tatgaatggc cgcccagaac cagcagtgcg ctagatacgc tgcttcgtga aattcagaac
      661 gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc ccaggacgtg
      721 aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt actggaagat
      781 caggagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga tttcaatatg
      841 ttgagcgata cttgggggca cagccaggtt gaagaacagt tcttcactct gacgaatctg
      901 ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca ccgcagtgat
      961 tttgctgcgc tgttaccgca ccggacgtta aaagaggcag agagcatcgc cggtcagtta
     1021 atcaaagccg tcgatacctt gccgaacaat aaaatgctcg atcgcgacga tatgatccac
     1081 attggtatct gcgcctggcg tagtggtcag gataccgagc aggtaatgga acatgcagag
     1141 tctgccacgc gtaatgcggg attgcagggc ggcaatagct gggctattta cgatgactcg
     1201 ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca aatgctcagt
     1261 cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat
     1321 cgcgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc ggagtatatg
     1381 ccgatggtct tgcagtttgg cttatcggaa gagtatgacc gtctgcaaat cagccgtctt
     1441 attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac cgttgagtcg
     1501 ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca
     1561 caacgaaaac gcataattat tgaacttgca gaggccgatg taggtcaaca tatcagtcgt
     1621 ttacaacctg ttattcgttt agtgaatgct ttaggggtac gggtagccgt caaccaggct
     1681 ggtttgacgc tggtaagcac cagttggatc aaagaactta atgttgagtt actcaagctc
     1741 catccggggc tggtcagaaa cattgagaag cgaacggaga accaactgct ggttcaaagc
     1801 ctggtggaag cctgctccgg gaccagcacc caagtttacg ccaccggtgt gcgttcgcga
     1861 agcgagtggc agaccctgat tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg
     1921 tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc ggtttaacct
     1981 gccgtttaat ctgttttcac gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt
     2041 ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg ttgcaaacag
     2101 gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt tgtaacaaag
     2161 gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg
     2221 tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat tatcccttag
     2281 tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc tgggtactgc
     2341 gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt ccgtggtggc
     2401 cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa
     2461 gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga aagacggcgt
     2521 tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag
     2581 caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca
     2641 ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct
     2701 gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg
     2761 ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct ccttgaacgg
     2821 cgtggtttac tcctcttccg tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa
     2881 ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc accgcagaac gtatcaagca
     2941 cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa
     3001 cctggcagaa ggtgttccac gcggttttac cctgaactcc aacgaaatcc tcgaagcact
     3061 gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc
     3121 ggaactggct tccgacatct ccgagcgcgg catggtgctc accggtggtg gcgcactgct
     3181 gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg ttgctgaaga
     3241 cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg
     3301 cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaaatgtct gtttaccctg
     3361 cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg tggcccgtcg
     3421 ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat tgccgacagc
     3481 cgcctgggga cgttcagtca aatccgtact tatatggata ccgccgtcag tcctttctac
     3541 tttgtttcca atgctcctcg tgaattgctg gatggcgtat cgcagacgct ggcctcgcgt
     3601 gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa
     3661 ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc
     3721 ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt taacgatcct
     3781 tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga aggccagccg
     3841 gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact gaccagtcgc
     3901 gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg caacgatatc
     3961 cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca tctgccagcg
     4021 aatacggata ttcgtgttgg tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg
     4081 gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg cgcttatact
     4141 gtgattcagg cgcgtccgac tgcaggactg caacgtttgc gttatctgct gctgctgtgg
     4201 ggcgcggatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg tgttgctaat
     4261 gaacgtctga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat ggggccaaag
     4321 ttacctgaac cggcaacggg gatcgctcag ccgactccgc agcaaccgac gacaggaaat
     4381 gcagctactg cgcctgctgc gcccacacag cctgctgcta atcgctctcc acaaagggct
     4441 acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga
     4501 gctatcgtag ccagggacgt tgggtaatct ggctctcttt cctcattgcg ctgttgctgc
     4561 aaatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg ttactcatct
     4621 tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt gggcacaggt tttgtgatgg
     4681 gtgccatact ggatctgatc agcggctcga cgcttggcgt acgcgtattg gcgatgagca
     4741 tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca ttatggcagc
     4801 aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc tgggcagagt
     4861 ttttagtgat taacgtctct ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg
     4921 tgctctggcc gtggattttc ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat
     4981 aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc aggagttact
     5041 tgcgcaactt ggcgtgacct ttgaacgtat tgttacaggc attgaggagc agcgtcagcc
     5101 gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc gagaaagcac aggcgggtgt
     5161 cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggat actatcgtta tcctgaatgg
     5221 agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc
     5281 gggtcagacc catcaggtga tgacggcagt ggcgttggcc gacagccagc acattctcga
     5341 ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca tcgcgggcta
     5401 tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac ggtattcagg ggctgggtgg
     5461 ctgttttgtc aggaagataa atggcagcta tcacgccgta gtcggcttac cgctggttga
     5521 aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata aacatgacgg
     5581 ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt ggcgtatatt gatggcggta
     5641 ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca
     5701 agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat attgggctgg
     5761 ataaagccgc gtttcttcat gcatccgata tcatgccgca caccgaatgt gtggcgggtg
     5821 aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc
     5881 tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg accaccgata
     5941 tcacgctgcc ttctcgctat ctggtgttta tgccaggggc ttctcacgtt ggggtttccc
     6001 aacgtattga aagcgaatca gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg
     6061 acgagcaggg cgggtttatc atccgtaccg cagcggaagg ggttggcgag gctgaactgg
     6121 cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt aaaaaacgcc
     6181 cgcagacccg ttatcagctg tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt
     6241 tcgccgacgc cgaactggac cgcattcgcg ttgactcacg cctgacttac gaagcattgc
     6301 tggagtttac ctcggagtac attcccgaga tgaccagcaa gctggagcat tacaccggac
     6361 gccagccgat tttcgatctc tttgatgtcg aaaacgaaat ccagcgagcg ctggaacgca
     6421 aagtagaact gaaatccggt ggttatctga ttatcgacca gaccgaagcg atgaccaccg
     6481 tggacatcaa taccggggcg tttgtcggtc atcgcaatct ggacgacacc attttcaata
     6541 ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgcaat ctgggcggga
     6601 ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgacgc gtgctgcact
     6661 cgctggagca ggcattgagc aaagaccggg tgaaaactag cgttaatggt ttttcggcgc
     6721 tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac gtactgtgta
     6781 acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg
     6841 aaatcatgcg cgagattgtt cgtgtccacc atgcttacga ctccgaccgt ttcctggtct
     6901 atgcttctcc ggcagtagct gaagccttga aaggcgaaga gtcacactcg ctggcggaag
     6961 tggaaatttt cgttggtaaa caggttaaag tacaaattga accgctctat aaccaggagc
     7021 agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa gggtttttga
     7081 gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat tttactgctt
     7141 actggagccg cgctcgttgt gatcgctgcc ctgctggtta gcggcctgcg tattgcttta
     7201 ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc gactggcatg
     7261 ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggcccgac gcttgaagca
     7321 cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg cgttactctg
     7381 gcgctggatg tctggcagag cttgttacat atgcgctggc agtttcgcga cctcactttc
     7441 tggcagctgc gctttcgcac caacactcct atcaccagcg gtggtggtaa tgatagcctg
     7501 gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga tctccgcgac
     7561 agtgaagtca gttttctgac gccatccggt cagcgcgccg agctggcgat cccacaactc
     7621 acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt
     7681 accggacagc acggcgtgat gcaggtgcgc atggatttgc gcgatgatga ggggttgtta
     7741 agcaatggtc gcgtctggct acaggcggat gacatcgacc tgaagccgtg gctcggtaaa
     7801 tggatgcagg acaatattgc tctggaaacg gcacagttct cccttgaagg ctggatgacg
     7861 atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg tgccagctgg
     7921 ttgggcgaga agcaaacgca tacgctgtcg gtggataatc tgaccgcgca tattacgcgt
     7981 gaaaatccgg gctggcagtt ctctattccc gatacacgga tcacgatgga cggcaaaccc
     8041 tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg cggcaaagac
     8101 aataaacgca gtgacgaact ccggattcgc gccagtaatc tggagctggc aggcctggag
     8161 ggcatacgcc cgctggccgc gaaactttca cctgcactgg gtgatgtttg gcgctccaca
     8221 caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc ggcagacaag
     8281 acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt attaccgggt
     8341 gcggaacact tctccgggac gctttccggc agcgttgaaa atggtttgct taccgcgtcg
     8401 atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactgga aatcgccgac
     8461 ggccaggcaa ctataagctg gctgaacaat gacaaaggtt tccagctgga tgggcgtaat
     8521 attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct
     8581 aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg ttcacaagcc
     8641 tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattactt aagtggcgcg
     8701 attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc gcaactcttc
     8761 ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt
     8821 gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga ctttattaac
     8881 gacggtttat ggatgaaaac cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc
     8941 accgcagtga tccctgacta ctcgaaagaa aaactgctga ttgacgctga cattaaaggt
     9001 ccgggtaaag ccgttggccc ttactttgat gagacaccgc tgaaagattc tctgggtgcg
     9061 accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct tgatatcccg
     9121 ctgaacggcg aactggtaac cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt
     9181 atcaaaccac tcgacagcac cctgaaaaat ttgagcggta aattcagctt tatcaatggc
     9241 gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa cgtggatttt
     9301 tccaccaaag aaggggcaaa agcctaccag gttgcggtga atctcaacgg taactggcaa
     9361 ccggcgaaaa ccagcgttct gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg
     9421 tgggatggta aagtgggcat tgatctgcct tatcatgctg gcgcgaccta taacgtagag
     9481 atgaacggcg atctgaataa tgtgagtagt cacttacctt caccgttagc caaacctgcg
     9541 ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt tgaattaacc
     9601 ggacaggctg gtgcggataa ccatttcaat agccgctggt tgcttggtca aaagctgacg
     9661 ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt
     9721 ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt ggctggccct gttccagaaa
     9781 ggcgctgcgg agagtgtcgg tggtgcagcg agtttcccac aacacataac gttacgtacg
     9841 cctatgttgt cactgggaaa tcagcaatgg aataacctga gtattgtttc gcaaccgacg
     9901 gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt
     9961 aataacgcgc cgtggctggc gaatatcaaa tatctttatt acaacccgag cgtggcgaaa
    10021 actcgtggtg attcaacgcc gtcatcacct ttcccgacaa cggagcgcat taacttccgt
    10081 ggctggccgg acgcacaaat acgatgcaca gagtgctggt tctgggggca aaaattcggt
    10141 cgcattgaca gtgatatcac catttctggc gatacgttaa cgctgaccaa tggactgatt
    10201 gatactggtt tctcgcggct tactgccgat ggtgaatggg ttaataatcc ggagaatgaa
    10261 cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg atgccgccgc agaatttttt
    10321 ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt acactggcgc
    10381 aaagcaccct ggcaaccaga tgaggcgacg ttgaatggca tcattcatac tcaactgggt
    10441 aaaggcgaaa ttaccgaaat caataccgga catgccgggc aattgctgcg cttattgagc
    10501 gttgatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg cgaagggttc
    10561 tattttgact ccattcgcag caccgcgtgg attaaagacg gggttatgca caccgacgac
    10621 acgctggtgg atggcctgga ggcggatatc gccatgaaag ggtcggtaaa tctggtacgt
    10681 cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt gggcgtggct
    10741 gcggcttttg cggttaaccc cattgttggc gcggcagtat ttgcagccag taaagtgctg
    10801 gggccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc gctggacgat
    10861 ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag
    10921 ggcgcgtaat tgccccaatc tcatatgata atcgttgcca aaggccaacg agccggaaca
    10981 taactgtagg ccggataggg cgttcacgcc gcatccggca gccgtaaaaa atcctctact
    11041 gcagtaacta acgagtagca aaaacgatga gtctgaacct ggtaagtgaa caattgctag
    11101 cggcgaacgg cctgaaacat caggacttgt tcgcgatcct cggtcaactg gccgaacgtc
    11161 gccttgatta tggcgatctc tattttcagt cgagctatca cgaatcctgg gttttagaag
    11221 accgcattat taaagatggt tcttacaaca tcgatcaggg cgttggtgtg cgtgcaatca
    11281 gtggtgaaaa aaccggattt gcttacgctg accaaatcag cctgctggcg ctggaacaga
    11341 gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga tggcaaagta cagacgctgg
    11401 gcgcggtaga gcatagcccg ttgtatacct cggtagatcc gctgcaaagc atgagccgtg
    11461 aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc ccgcgaagcg gacaagcgcg
    11521 tacaagaagt gactgccagc ctcagcggcg tttatgaatt aattctggtt gcggccaccg
    11581 acggcacgct ggcggcggat gttcgtccgc tggtgcgtct ttccgtgagc gttctggtcg
    11641 aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt
    11701 tcttccttgc cgatcttgac ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc
    11761 gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc gggcaccatg ccggtagtac
    11821 ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg
    11881 acttcaaccg ccgcggcact tcagtattta gtgggcaggt cggggagctg gtggcttcag
    11941 aactgtgtac cgtggttgat gacggcacga tggtcgatcg ccgtggttcg gtggcgattg
    12001 atgacgaagg tacgccaggc cagtacaacg tgctgattga gaacggcatt ctgaaaggct
    12061 acatgcagga taaactcaac gcgcgtttga tggggatgac gccgactggc aacggtcgcc
    12121 gtgaatccta cgcccatctg cccatgccgc gtatgaccaa cacctatatg ctgccgggta
    12181 aatcgacccc gcaggaaatt attgaatccg ttgagtacgg tatctatgca ccgaactttg
    12241 gtggcggtca ggtggatatt acatccggca aattcgtttt ctccacttca gaagcgtatc
    12301 tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc gacgttgatt ggttccggta
    12361 tcgaaaccat gcagcagatt tcgatggttg gcaacgacct gaaactggat aacggcgtgg
    12421 gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt gggccagcca acgctgaaag
    12481 ttgataacct gactgttggc ggtactgcgt aataatttca ttattttcag aaggataatt
    12541 aatctttcta cgtgcacgaa cggtcccctc gctcctctgg ggttagggtg aggggaaccc
    12601 gttggcacag gtttgaacaa cgtaacagta caatatgaat tacttctctt tcccgcgccc
    12661 atgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg agttgatcac
    12721 gacctgtacc ttcagcggca gtttatcttt ttcggtatac aacgcataaa ccgggcgtgg
    12781 atctgactgg taacgcggca gcaggatctc cagctcccca cgattgatct cgttgatcac
    12841 ccacatcagc ggcacgtagg cgatcccggc acccgccgtc agccagcgca ccagcgtcat
    12901 cggatcatta gtcacaaatc ttccttgtgg gatcaggcga gtcgagatcc cttccggtgc
    12961 gatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat gactactcaa
    13021 atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg cgcacaccac
    13081 catcggcatc gcgcccagac ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg
    13141 gattaccaca tccagaccat cggcaatcag gtcgggggca ggaattccgg taaccagatt
    13201 gacgctcaaa cctgggtatt ctttcagcat ttttgctgtc agcccggcga gaacattttg
    13261 tgccatagtt gaagaacagc caatgcgcag cgtcccgatg ggggtgttat tgaaggcata
    13321 cagttgctca tgaacatcct gcacttcatg gagcatccga cggcagccct ggtagtaaat
    13381 tctgccggct tcagtcagac caatgctgcg tgtactacgg tttaacagct ttacctgcaa
    13441 ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct gtagctgtct
    13501 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
    13561 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
    13621 taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
    13681 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctatacaa
    13741 ccaaggtcga catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
    13801 tattttttga attgctttta tcactggcga ttttctggct ggtgcgccgg gtacttgtgc
    13861 caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
    13921 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
    13981 aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
    14041 aatgcctggg tctattacac cgaatccccg tggacgcgtg acgcgcgctt tagcgctgat
    14101 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcatgataac
    14161 cagctggtga aaaaaggaca ggtactgttc accatcgacc agccgcgcta tcaaaaggcg
    14221 cttgaggaag cgcaagccga tgttgcttat tatcaggtac tggcacagga gaaacgccag
    14281 gaggccggac gtcgtaatcg tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag
    14341 gccaacaacg tactacaaac ggttctgcat cagttagcga aagcgcaggc gacccgcgat
    14401 ctggcaaaac tggatcttga acgcacggtg atccgcgcgc cagcggatgg ctgggtgacc
    14461 aacctcaacg tctataccgg tgagtttatt actcgtggat caacggcggt tgcgctggtg
    14521 aaacagaact ctttctatgt actggcctat atggaagaaa ctaagctgga aggggtgcgc
    14581 cctgggtatc gtgcagagat cacgccgctt ggcagtaaca aagtgctgaa agggactgtt
    14641 gatagtgttg ccgctggggt caccaacgcc agcagcacgc gtgacgacaa agggatggcg
    14701 accatcgact ctaaccttga atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt
    14761 ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacagt ggtggtcact
    14821 ggcaaacaag atcgcgacga aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg
    14881 cgtgagtttg gttaatcacg atgggtattt tctccattgc taaccaacat attcgctttg
    14941 cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac ttccagctgg
    15001 aaacgccacg ctgggcggta ctgacagcgg cgattgttgc tgccggtccg gcctttgctg
    15061 cgggaggtga accgtattct ggcgctattc gctatcgtgg ctttttgcgc atcatcggca
    15121 catttattgg ctgtattgcc ggactggtga tcatcattgc gatgatccgc gcaccattat
    15181 tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc tcgctggtac
    15241 gaatagaaaa ctcgtatgcg tgggggctgg ccggttatac cgcgctgatc attgtgatca
    15301 ccattcagcc tgaaccattg cttacgccgc agtttgccgt cgaacgttgt agcgagatcg
    15361 ttatcggtat tgtgtgtgca attatggcgg atttgctctt ttctccgcga tcgatcaaac
    15421 aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta atgcaactct
    15481 gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgacctg gttcgacgca
    15541 ccacggcgct acaaggtatg cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg
    15601 ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc caatcctgcg
    15661 aaacttatct tattcagaat acacgcccgg aattgatcac tgatactttc cgcgaatttt
    15721 ttgacacacc ggtagaaacc gcgcaggacg tccacaagca gctcaaacgc ctgcgaagag
    15781 ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg
    15841 caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg
    15901 aagaagagat cctgcaaggc gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg
    15961 caatggttaa cttctggcga accacacttt cctgcattct gggaacgctt ttctggctgt
    16021 ggacgggctg gacttccggt agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg
    16081 caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctacggg acgctggccg
    16141 cgctgccgtt agggctgctc tactttttgg tgattatccc taatacccaa cagagcatgt
    16201 tgctgctgtg tattagcctg gcagtgctgg gattcttcct cggtatagaa gtacagaaac
    16261 ggcgactggg ctcgatgggg gcactggcca gcaccataaa tattatcgtg ctggataacc
    16321 cgatgacttt ccatttcagt cagtttctcg acagcgcatt agggcaaatc gtcggctgtg
    16381 tgctcgcgtt caccgttatt ttgctggtgc gggataaatc gcgcgacagg actggacgtg
    16441 tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat gtggcacgtc
    16501 gtaaagagaa ccacctcccg gcactttatc agcagctgtt tttgctgatg aataagttcc
    16561 caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcccac cagcgcctgc
    16621 gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca
    16681 cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac tttggtcagt
    16741 tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagct ccaccgcagg
    16801 tgacggaacc ggttcatcgg ctggcgggga tgctccataa gtatcaacat gcgttgaccg
    16861 atagttaagt caaaaccgac gccaaaagcg tcggtttttt catggctata cttagcgatg
    16921 cacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta tacctttgat
    16981 tttgatgaga ttgagagtca ggaggatttt tatcgtgact ttagccaaac ctttggtctg
    17041 gcgaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa cgatgtcctg
    17101 ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg ccgttttggc
    17161 gcgttaatat tgctgtttga tgaggcagag gaagagctgg aagggcattt gcgttttaat
    17221 gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc aatatcgtcg gacaagacga
    17281 tgagggttta tttgtacagt tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag
    17341 taatctggta ggccgttgcg cctttctctt ccgctttttt gttcagcatt tcacgcatat
    17401 ccattggcga agacgccaca ccacttacgg atacggtccc gattgcttca cgattttgtg
    17461 cttgtgcagc atcaatggag tcggcagcga atgcaccgaa agagagaaca gaaagtacgc
    17521 ttaatgcagc aacagtggtt ttgattttca tgatttttac ctcgacataa tcttttagct
    17581 gggcctttgt ttcgtgaccc atatcacaaa atcaagtata cactaattat tg
//
LOCUS       DAPHDX010000094        17596 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPHDX010000094 DAPHDX010000000
VERSION     DAPHDX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.095"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            131..1270
                     /gene="arnB"
                     /locus_tag="RH058_003128"
     CDS             131..1270
                     /gene="arnB"
                     /locus_tag="RH058_003128"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV3138684.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1274..2242
                     /gene="arnC"
                     /locus_tag="RH058_003129"
     CDS             1274..2242
                     /gene="arnC"
                     /locus_tag="RH058_003129"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV3138685.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2242..4224
                     /gene="arnA"
                     /locus_tag="RH058_003130"
     CDS             2242..4224
                     /gene="arnA"
                     /locus_tag="RH058_003130"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV3138686.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4221..5111
                     /locus_tag="RH058_003131"
     CDS             4221..5111
                     /locus_tag="RH058_003131"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV3138687.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5111..6763
                     /gene="arnT"
                     /locus_tag="RH058_003132"
     CDS             5111..6763
                     /gene="arnT"
                     /locus_tag="RH058_003132"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV3138688.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6760..7095
                     /gene="arnE"
                     /locus_tag="RH058_003133"
     CDS             6760..7095
                     /gene="arnE"
                     /locus_tag="RH058_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV3138689.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7095..7481
                     /gene="arnF"
                     /locus_tag="RH058_003134"
     CDS             7095..7481
                     /gene="arnF"
                     /locus_tag="RH058_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV3138690.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7475..7741)
                     /gene="pmrD"
                     /locus_tag="RH058_003135"
     CDS             complement(7475..7741)
                     /gene="pmrD"
                     /locus_tag="RH058_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV3138691.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7851..9206)
                     /gene="menE"
                     /locus_tag="RH058_003136"
     CDS             complement(7851..9206)
                     /gene="menE"
                     /locus_tag="RH058_003136"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV3138692.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9203..10165)
                     /gene="menC"
                     /locus_tag="RH058_003137"
     CDS             complement(9203..10165)
                     /gene="menC"
                     /locus_tag="RH058_003137"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV3138693.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFVRETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10165..11022)
                     /gene="menB"
                     /locus_tag="RH058_003138"
     CDS             complement(10165..11022)
                     /gene="menB"
                     /locus_tag="RH058_003138"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV3138694.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11037..11795)
                     /gene="menH"
                     /locus_tag="RH058_003139"
     CDS             complement(11037..11795)
                     /gene="menH"
                     /locus_tag="RH058_003139"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV3138695.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11792..13462)
                     /gene="menD"
                     /locus_tag="RH058_003140"
     CDS             complement(11792..13462)
                     /gene="menD"
                     /locus_tag="RH058_003140"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV3138696.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13551..14846)
                     /gene="menF"
                     /locus_tag="RH058_003141"
     CDS             complement(13551..14846)
                     /gene="menF"
                     /locus_tag="RH058_003141"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV3138697.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14925..15230)
                     /gene="elaB"
                     /locus_tag="RH058_003142"
     CDS             complement(14925..15230)
                     /gene="elaB"
                     /locus_tag="RH058_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV3138698.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15285..15746)
                     /locus_tag="RH058_003143"
     CDS             complement(15285..15746)
                     /locus_tag="RH058_003143"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3138699.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFISVTEVYEEDGIPHIGMAREVIQA"
     gene            15811..16728
                     /gene="rbn"
                     /locus_tag="RH058_003144"
     CDS             15811..16728
                     /gene="rbn"
                     /locus_tag="RH058_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV3138700.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16916..17572
                     /locus_tag="RH058_003145"
                     /pseudo
     CDS             16916..17572
                     /locus_tag="RH058_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         4154 a         4733 c         4424 g         4285 t
ORIGIN      
        1 gtctaatatt agtttcttaa ggttaagtta atattctatc cttaaaattt cgctccaaat
       61 ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga
      121 aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact
      181 cgctgcagtt aaagaggttc tcgaatccgg ttggctcaca accggtccga agaatcaggc
      241 gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc
      301 caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat
      361 tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgttgg gtgcaacgcc
      421 ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgagtcagc
      481 cataacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat
      541 tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca
      601 tgccgtcggt acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc
      661 atttcatgcc attaaaaata ttacctgtgc ggaaggtggc ctgattgtaa ctgataatga
      721 aaaccttgcc cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga
      781 cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa
      841 tctgaccgat attaacgccg caattgccct gacacagtta gcaaaattag agcacctcaa
      901 cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca
      961 gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta ttattcgtgt
     1021 cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg
     1081 cattggtacc gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt
     1141 tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt ctttgccgct
     1201 gttcccggat atgactaccg ccgatgccga ccgtgtcatc acagcccttc agcaactcgc
     1261 aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt
     1321 ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc
     1381 gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca
     1441 tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg
     1501 caattacggg caacattcag cgattatggc gggattcagt cacgttaccg gcgacttaat
     1561 tattaccctt gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa
     1621 agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg acagctggtt
     1681 tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag cgcaccaccg gcaaagcgat
     1741 gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca
     1801 ctgccatgaa cgtagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgccat
     1861 tgaaattcca gtacatcatt ccgagcgtga gtttggtgaa tccaaataca gttttatgcg
     1921 cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct
     1981 gagtctgctc ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat
     2041 tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt
     2101 gctgtttact tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg
     2161 caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc
     2221 atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc
     2281 ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc
     2341 gataatcccg gtgaaaaagc cttttatggt tcggtggctc gtctggcagc ggaaagaggc
     2401 attccggttt atgcgccgga tgacgttaat catccgctgt gggtggaacg cattgcccaa
     2461 ctgtcgccag atgtgatttt ctctttttat tatcgccatc ttatttgcga cgaaattttg
     2521 cagctcgctc ccgcaggtgc atttaatctg catggttcgc tgttaccaaa atatcgtggt
     2581 cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac
     2641 cgaatggtaa aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg
     2701 ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg
     2761 gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac
     2821 gaagccacct gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg
     2881 gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc
     2941 tatgttggca atcagaaatt taccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa
     3001 gcacagccgg ggagcgtgat ttctattgca ccgctgttga ttgcctgtgg cgatggcgcg
     3061 ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg
     3121 cagacgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga
     3181 cgccgtaccc gggtactcat cctcggggta aatggcttta ttggcaacca tctgacagaa
     3241 cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata
     3301 agccgttttc tgaatcatcc gcattttcac tttgtcgaag gcgatatcag tattcattcc
     3361 gaatggattg agtatcacgt caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc
     3421 acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat
     3481 ctgcgcatta tccgctactg cgtgaagtac cgtaagcgaa tcatcttccc gtcaacttca
     3541 gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc
     3601 ggcccggtga ataaaccacg ctggatttat tcggtatcaa aacaattact tgatcgggtg
     3661 atctgggcct atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg
     3721 atgggaccgc gactggataa ccttaatgcg gcacgaatcg gcagctcccg cgctattacg
     3781 caactcattc tcaatctggt agaaggttca ccgattaagc tgattgatgg cggaaaacaa
     3841 aaacgctgct ttactgatat tcgcgatggt atcgaggcgt tataccgcat tatcgaaaat
     3901 gcgggaaatc gctgcgacgg cgaaattatc aacattggca atcctgagaa cgaagcgagc
     3961 attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat
     4021 ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat
     4081 caggacgtag agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag
     4141 ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt
     4201 gatcttacgg ataaaccatc atgaccaaag taggcttacg cattgatgtc gatacctttc
     4261 gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg
     4321 ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga
     4381 agccacagtt tttgtggaag atgctgcgct caaatgcggc atcgctttat ggctgggata
     4441 ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca
     4501 ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac catgcctggc
     4561 aagcccgtag cggtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc
     4621 gcactctgga agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg
     4681 ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc
     4741 gtggggccat gccgttccgt ccattgctcg aatcaggaaa ccctggcact gcgcaaattc
     4801 cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta
     4861 acggttggtt actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg
     4921 cagaagttga aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc
     4981 aggaaggcgt gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac
     5041 aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg
     5101 gtagtcgctg atgaaatcgg tacgttacct tatcggcctc ttcgcgttta ttgcctgcta
     5161 ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga
     5221 aatcagtcga gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg
     5281 ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg
     5341 cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct
     5401 ggtgacctgg tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt
     5461 aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt
     5521 tattgcattc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac
     5581 gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat
     5641 gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac
     5701 gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact
     5761 gacggttctc ccttggggac tggcgatagc gcagcgggag cctgacttct ggcactattt
     5821 tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata gagctccgtt
     5881 ctggtactac gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg
     5941 tgcactgtac acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg
     6001 gacgataatg ccgctgctgt ttttctccgt cgctaaaggt aaattgccca cctatattct
     6061 ttcctgcttt gcatctctgg caatgctgat ggcgcattac gctttgctgg cagcaaaaaa
     6121 taatcctctg gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat
     6181 tattgccaca tttgtggtct ccccgtgggg accaatgaac acgccggtgt ggcaaacctt
     6241 cgagagctat aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg
     6301 gtacacctta acaaacgtcg aaaagacctg gccttttgcc gcgctttgcc cgctggggct
     6361 ggcgttgctg gtaggatttt caattcctga cagagttatg gaaggaaaac atccgcaatt
     6421 ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt
     6481 cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca tgtatcgcca
     6541 gacaggtgag ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg
     6601 tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta ctctcgtgct
     6661 ttcggttgac cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcga
     6721 tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc
     6781 tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg
     6841 gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt
     6901 ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac
     6961 ccgatgttaa gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa
     7021 ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc
     7081 gggagtacgg tgtaatgggc ctgatatggg gattattcag cgtcattatt gcctcagttg
     7141 cgcaattaag cctgggtttt gcggcaagtc atctgccgcc gatgacgcac ttatgggatt
     7201 ttatcgcggc tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg
     7261 gttatctgct ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag
     7321 cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg
     7381 gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt
     7441 tgatgctgat tttcctgccc acgacaaaac aacgttactg agttttccct gccgctgtac
     7501 actgccgttc ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca
     7561 gcttttcacg attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt
     7621 tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga
     7681 gcataagtac atgtctgtta tcttgtttat tgcagcacga ttttttgacc agccattcca
     7741 ttgcattatc ctgtttgcta agagttttca catcaattta cagattaaca gataaactct
     7801 ttatcaacgg caatggaatg gcagaaatga gaagtgaaac ggagggttaa ttattgctga
     7861 cgttgcaccc actcttttag cgcctgacgt gaaattttaa taccgccgtt tttcagctcc
     7921 ggcggtaaag tcagccagcg caccggttgt tgaaaacgag ccagcttatc tttcacccat
     7981 tcgccaagat caacggtttg ctggtcatac tccaccaccg ctaccggccg atgaccaaac
     8041 tccttgtcgg caacgggaac gataaacacc tgcaaaaccg caggatgtgc agcaataaca
     8101 cgctcgactt cttccggctg aataccctca ccgccactga agaacagatt gtctaaacgt
     8161 ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct
     8221 tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt aaccttctgc catactggca
     8281 gcccgcagcc acacttcatt attaacgatt ttcacttccc gacccggcag cggcgaacca
     8341 acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg aggcaaactc ggtcagacca
     8401 tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata
     8461 gccgcgccgc caagtaacac cgctttcagg gaaacggaac tgcggttaac cagcaaacgc
     8521 cagagttgtg ttggcaccag tgaagcgtga gtacagcctg ccagcatttg ctccaatggc
     8581 tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc agcgccacat aattccctga
     8641 ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc aaacggaatc
     8701 aacgataaca ccccttcggc actggcaaga tgggcctgat aagtatggac agcggctttc
     8761 ggcaacccgg tagaacccga ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgta
     8821 gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg ttaacgccgg aaacgtgttt
     8881 tccccatccg gcaccagcgc aaattgcagc gtcagattgg gcagcaattc ttcaagcaac
     8941 ggttgcggca gctgagggtt cacgggcaac acccgcgccc cgcattgcag taacgccagc
     9001 caggcgagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct gccctcaaca
     9061 acgccctgcg ccgcaaagcc tgaggctaat tcatcgacgc gagcacaaag ctcgcgccag
     9121 ttaagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg ccgccagtga
     9181 cgccacggcc agtcagagaa gatcataaca accgctccag tgcatcaact tccacgacag
     9241 gcagcgtgct acccggccag cgacgtacct gctgcgcctg catcagatcc agcgtgtcca
     9301 gccctggaat ggtgtccggc gttaaccagg cggcaatccg cgccagttgc gttaagccta
     9361 agctcgattc aatggaagaa ctgatcaccg ccgtcagccc cagcgcgtgc gccgcctgta
     9421 cctgctcgcg tactttctcc agactgcccg tgagcgtggg tttgataact accgcgcgca
     9481 cgccctcttc agccacaaag gcaaaatccg gctcgcgcaa gctttcatcc caggcaatgg
     9541 caatgccggt ttcacggaca aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga
     9601 gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc tggcctttca
     9661 gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt gcctccagca
     9721 acagattaac caccatgccg tcgcgcacgg cttcgtacaa tcccactttg accttcgcca
     9781 ctttctcgcc tggcatatca gcaagtttga ggatcagatc gtcaggatcg ccattacaca
     9841 gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac
     9901 agcttacgcc aaaggcaacg gaaggcatct gcggtagctc gcaatcgcct gccagccagt
     9961 tatttaccca ggcaagcagc acgctttgcg cctcttccca ggtttcctgg ctgaagcccg
    10021 gcagtgggga gatctccccc cacccttcgc gctcgccctc acgcaggcaa acgtacagcc
    10081 cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc
    10141 agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga aatcaggctg
    10201 acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt agaacagcat
    10261 ggtggcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg cgttcagcgc
    10321 agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcgcggc accaacggac
    10381 ggtttctttt tccagatccg ccagcggtac cacggtgttg acaaggccca tatccagcgc
    10441 ctgttttgcg tcgtactgac ggcacaggaa ccagatttct cgcgcttttt tctgcccgac
    10501 gatacgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt tcgggccagt
    10561 ctgaccgaag atggcattat ctgctgcgat agtcaggtcg cacatcatgt gcagaacgtg
    10621 accaccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac aggtacggat
    10681 ctgacgctgg aagtccagca cgttcaagtg atgtacgccg gaatcatctt tatagccgcc
    10741 gtaatcacca cgcactttct ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt
    10801 cagaatgatc acgccgatat tgtcgtcata acgcgcatct gccagtgcct ggatcatctc
    10861 ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg tgatttttgc
    10921 gataccgtcg gtggattttt cataacgaat gtcctcgaaa ccttcggagc agtcgtgcca
    10981 ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc ctttagtcag
    11041 aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg atgcgcgtta
    11101 tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag ggcgcggaat
    11161 ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcact aaggttagca
    11221 cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag ggttgcgcca
    11281 ttattgttgc tgcgcagcgc caccagctcc cggcgttgat cgtcattgag cgaggcaaaa
    11341 acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgca aaaacgctgc
    11401 gcccattggc gatcggaacg ctgacgttcc gcacgttgtt cagcattttg cagccccgga
    11461 tgcccgcctt cgacaataac cccacaaagc cccgccagcc cctggcaagc cgccatcatc
    11521 gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt gtaactaacc
    11581 aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat cgccgccgaa
    11641 ccaccatgac ctgggagatc aacatacaac cgtgagtagt cggcaaacgc ctcgcccact
    11701 tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct
    11761 ggttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc gccagaagtt
    11821 gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc gtggtggttg
    11881 gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc ggacgatgat
    11941 atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc atcagataga
    12001 aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg ccgttgttgt
    12061 tcaccacaat taataccagc ggcgcagaaa cctgacgcaa taacgccagc gcgttgagat
    12121 cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa
    12181 cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc ccctcggttg ctgtacaccg
    12241 ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg ctgttaccaa
    12301 caaacaattg cccctgttca ggcagatagt cgcagatgcg atgcgccagt tgcgcttcgc
    12361 caaacgcatc acggcgggca ataaccgcct gcattgcctg ttccgccagg cgcgggattt
    12421 caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg gcaatattgg
    12481 caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa
    12541 tccagtactc ttctggttca cagcttgcct gccattgcag gagccgtttc cccgtcaggc
    12601 tgcttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct ttggcattgc
    12661 ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca tcgccaatca
    12721 gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc gcactcatgc
    12781 gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc tgtttttcac
    12841 tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag tcacccagac
    12901 gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca gcaaacgggc
    12961 agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg gtagaaacca
    13021 gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc gtggggtgag
    13081 aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt agctccggcg
    13141 ggcgatcggc ggttaacagg atcagttttt ctccggttaa cccagcttca atcagtgccg
    13201 gatagagatt tgctaccgcc gtgccggagg tcacaatcac cgccaccggc tgcttgctga
    13261 ctttcgccag ccccagcgcc agatgtccca gtccacgctc atcgaaatgg gtgtggtgaa
    13321 tgaatgtgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag ccaggggcga
    13381 tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc
    13441 ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct aagtataagg
    13501 agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta ctattccatc
    13561 tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc ttgctcgggg
    13621 tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc gctaattttt
    13681 gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc cgcagaaccg
    13741 gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc
    13801 ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac atcatccgct
    13861 ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag acgcagtacc
    13921 tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca gatatcttcc
    13981 accaccagca tgttctcgcg ctggttttta tcatccgcca tcagccactc tcctaactgc
    14041 tgcgcctgct tatcatcagg attatttgct actgttcccg ccagcgcttc agtacgcagc
    14101 gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc attttcgcca
    14161 tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc agccatcatc
    14221 gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag caccactttg
    14281 tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt
    14341 ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa gggcttgata
    14401 ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg
    14461 ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc caggcggggt
    14521 aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg taagtcggcg
    14581 tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc
    14641 gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac
    14701 gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata
    14761 tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg cagtagattt
    14821 tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc tcacattata
    14881 cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc
    14941 aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca gggtttttcg
    15001 tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata actgtctgaa
    15061 gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc tttcagctca
    15121 acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag tgtttcacta
    15181 agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat tctcgttctc
    15241 catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc ctgaattacc
    15301 tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt cacagagata
    15361 aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta aacaggctta
    15421 tcaggccagt gatgcgtaca tgtttcgagt gttttactca tcagctgctg ccccactttt
    15481 tcgccacgca acgcttcact gacaatcacc cgacctataa cgaccggctc aagatcatca
    15541 tcacttttca gaatcctcgc atacgccacc agttcatcat ttttccaacc gagaatatgg
    15601 cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt ctgctcgaca
    15661 acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga taattcagag
    15721 tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc cttatactag
    15781 ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg tacttcagcc
    15841 ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca acatccgacc
    15901 cagagcggac tttggttgtt tgactgcggt gaaggcaccc agcatcagct actgcatacc
    15961 gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg cgatcatctt
    16021 tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca acccttaacg
    16081 atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat tagcggctca
    16141 tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct cgatgatggc
    16201 ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg ctatcgtatt
    16261 gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc tggcgtgccg
    16321 cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga agatggaagg
    16381 cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct cgctattttc
    16441 ggcgataccg gcccctgcga tgccgcactt gacctggcta aaggtgtcga tgtcatggtg
    16501 cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg ccatagctct
    16561 acacgccagg ctgcgacact agcccgtgag gctggagtcg gtaagctaat cattacccac
    16621 gtcagctcgc gctatgatga caaaggttgt cagcacctgt tacgtgaatg caggtcaatt
    16681 ttcccggcga ctgaactagc gaatgatttc accgtgttta acgtttaact ctgcttatta
    16741 ttaaaaacag ggcgaaactt gccctgttat cgcaacccgc gctacatata tccacccaca
    16801 cgggcatatt gagcgatttt tgatatttat tcatctcctt acgaactata ttcagagcaa
    16861 taagttgcct ctttttatct tcattcagca caacctttgc aactaaaagg agcccatgat
    16921 ggttacagtt gtcagcaatt attgtcaatt atctcaaacc caactcagtc agacatttgc
    16981 agaaaaattt actgtgaccg aggaattact gcagtcttta aaaaaaacag cgttatccgg
    17041 agatgaagaa agtattgagt tactgcataa tattgcgtta ggttatgatg aatttggaaa
    17101 aaaagctgaa gatattcttt accatattgt tagaaaccca acaaatgaga ccctatcgat
    17161 tatccgactt attaaaaatg cctgtttaaa attatataat ttggcacata ccgcaaccaa
    17221 ctcccacctt aaaccaactg gtccggataa ttcagatgtt ctcctgttca aaaagctatt
    17281 ctccccttcg aaattaatga caattatcgg tgatgaaatt cctcttatat ctgaaaaaca
    17341 gtcgctttca aaggtgcttt taaatgatga gaataatgaa ctgagtgatg atacaaactt
    17401 ctgggataaa aatcgtcaat taaccacaga tgaaatagct tgctatcttc agaagatcgc
    17461 cgctaatgca aaaaatactc aagtcaatta tcctactggt ctctacgtcc cctactccac
    17521 cagaactcac ctggaagacg ctctcaatga aaatattaag agcgatccat catgaccgaa
    17581 tgaagtccag ttattc
//
LOCUS       DAPHDX010000095        16600 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPHDX010000095 DAPHDX010000000
VERSION     DAPHDX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.096"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(396..1592)
                     /gene="hemY"
                     /locus_tag="RH058_003146"
     CDS             complement(396..1592)
                     /gene="hemY"
                     /locus_tag="RH058_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV3138701.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1595..2824)
                     /gene="hemX"
                     /locus_tag="RH058_003147"
     CDS             complement(1595..2824)
                     /gene="hemX"
                     /locus_tag="RH058_003147"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV3138702.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(2846..3586)
                     /gene="hemD"
                     /locus_tag="RH058_003148"
     CDS             complement(2846..3586)
                     /gene="hemD"
                     /locus_tag="RH058_003148"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV3138703.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3583..4524)
                     /gene="hemC"
                     /locus_tag="RH058_003149"
     CDS             complement(3583..4524)
                     /gene="hemC"
                     /locus_tag="RH058_003149"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV3138704.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4911..7457
                     /gene="cyaA"
                     /locus_tag="RH058_003150"
     CDS             4911..7457
                     /gene="cyaA"
                     /locus_tag="RH058_003150"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV3138705.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7497..7817)
                     /gene="cyaY"
                     /locus_tag="RH058_003151"
     CDS             complement(7497..7817)
                     /gene="cyaY"
                     /locus_tag="RH058_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV3138706.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            8280..8483
                     /locus_tag="RH058_003152"
     CDS             8280..8483
                     /locus_tag="RH058_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3138707.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8520..9344
                     /gene="dapF"
                     /locus_tag="RH058_003153"
     CDS             8520..9344
                     /gene="dapF"
                     /locus_tag="RH058_003153"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV3138708.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9341..10048
                     /locus_tag="RH058_003154"
     CDS             9341..10048
                     /locus_tag="RH058_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV3138709.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10045..10941
                     /gene="xerC"
                     /locus_tag="RH058_003155"
     CDS             10045..10941
                     /gene="xerC"
                     /locus_tag="RH058_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV3138710.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10941..11657
                     /gene="yigB"
                     /locus_tag="RH058_003156"
     CDS             10941..11657
                     /gene="yigB"
                     /locus_tag="RH058_003156"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV3138711.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11741..13903
                     /gene="uvrD"
                     /locus_tag="RH058_003157"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11741..13903
                     /gene="uvrD"
                     /locus_tag="RH058_003157"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV3138712.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14050..14814)
                     /locus_tag="RH058_003158"
     CDS             complement(14050..14814)
                     /locus_tag="RH058_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV3138713.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15184..16134
                     /gene="corA"
                     /locus_tag="RH058_003159"
     CDS             15184..16134
                     /gene="corA"
                     /locus_tag="RH058_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV3138714.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16176..16598)
                     /locus_tag="RH058_003160"
     CDS             complement(16176..16598)
                     /locus_tag="RH058_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDV3138715.1"
                     /translation="MRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKN
                     QHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINE
                     YPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKKDNF"
BASE COUNT         3798 a         4303 c         4452 g         4047 t
ORIGIN      
        1 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
       61 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
      121 tctctgtggc ataataaacg ggtagatgct cattccatct cttatgttcg ccttagtgcc
      181 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
      241 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
      301 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
      361 ggtcccggag gtgcttgcct ccgggtgaga aggaactact gtggtgggtt gttctgcaac
      421 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
      481 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
      541 aaggcgagcg atgcttcctg ccattctccg tgcttcatca gtgactggcc cagtgtgctc
      601 cacaacagcg ggcgatcgcc gacgtttttg atctgctggc gcagcacttt ttccagctgt
      661 tccggattgt tggttttcag tcgaggaatc ggcagcagta ggcgatcgtc gtactggcgt
      721 ttcaggccat cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
      781 tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg gtttttccac
      841 cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag gccaatccat
      901 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
      961 gggatgatat ccagcagaga actccatgca cctgtgcgga tatacgcctg ttccgccaga
     1021 cgtaatactt ccggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     1081 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     1141 tcattgccgg ccagctctgc tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca
     1201 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc cgcgtgatcg
     1261 gcatttttcg ccatcagctt ttcaacttgc tgataatcgc cttccgccag tttcagcagc
     1321 gcctgttcgg tctgcttacg ggcacggcga cgcttacgtc cgacaaacca cccacgggtg
     1381 tgtgcgcctg tgcggaagat ccgccgcagt agccactcaa tggcaaacag cactaccatc
     1441 gccagaatca aaataatcgc caggcccgtg acgctggttt cgatattgta gttgtcagtc
     1501 tggatcagca cgtaaccctg atgaccggca atcatcgggc caaccactat ccccgcaatc
     1561 agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     1621 gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg
     1681 caggtgcagg tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac
     1741 gcacgcgggt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat
     1801 ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg
     1861 catcatcagt atcgtagtaa gctcgtaccc aggtggagac gttctccagc gcctggcgat
     1921 aagtctcttc ctggtgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat
     1981 tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac
     2041 ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc
     2101 attcgctgat ggaactggaa agctcttcac cgtcggaatc catcggcgaa ccatcgctgt
     2161 cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga
     2221 tgccgtcata atccacctgc gaaactgcag aaaggctggc gatatcatcg gtaattgccc
     2281 gacgaacggt aatcagactc gggtcattca tatccgccag gctggcatct gcacttttca
     2341 gcaacgctgc agcggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca
     2401 ccagaaaatc ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga
     2461 ccttttgttg gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct
     2521 taagctgtgc agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg
     2581 ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg
     2641 cctgttgttt accccagcca tacaaaccga cgcccgccgc cagagcaata gcgatagcca
     2701 ccgcgctgag aatcaatgcg gtattgttct tactcttttt ttctgttgcg acaggttgtg
     2761 acgtggtgtc cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg
     2821 tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag
     2881 cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct
     2941 cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca
     3001 gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg
     3061 attgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt
     3121 cacaaaaagt gacctcagca ccgcgcgctg tcagggtatc cccaattagc tcacgaccgc
     3181 cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta
     3241 gcaagacttc gctgatttcc cgatcctgcg ggtagagaat cttctgtccg cttacggtat
     3301 gtagcgccag tgcggtggtg cgtccaatgg cgaaataatc aggtagtcgg ggccatttac
     3361 gatcttgctg atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca
     3421 acagatcgct ctcccccagc gccgccagtt gatcagcaag ttgcggtaat tgtcgacccg
     3481 gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca
     3541 ctaactcttc tccagcggga gacgggcggg tgaccaggat actcatgccg gggcgtctcc
     3601 gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga
     3661 aatccccatt tgttcggcat tttgcggcgc accgcggcgt tcaccgcgaa taatctgcga
     3721 accgtccggc gcgccgacca gcgcacgcag ccagatttca ccatcaataa gctcggcgta
     3781 gctaccaatt ggcacctgac atccaccttc gagacgggta ttcatggcgc gttctgcggt
     3841 aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgcgtatc
     3901 atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg
     3961 tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag
     4021 aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg
     4081 cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact
     4141 ggacgtcccg acgatactgc ctgccggtaa cgcatccaga ctgtcataat tattggacac
     4201 aaaggcatcg cgaggatctt cacgctcaca aatagtgacc aaccccagac cttgcgggaa
     4261 ttcaaccggc acatctttca ttgaatgtac ggcgatatcg gcgcgattct ccaggagcgc
     4321 gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag
     4381 aatcacatcg ccgcgcgtca ccatcggtac cagttcaacc accaggcccg gatggctcgc
     4441 catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc
     4501 aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta
     4561 acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta
     4621 aaatgaggtc tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac
     4681 agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag
     4741 gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc
     4801 gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt
     4861 gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct
     4921 atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc
     4981 ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact
     5041 atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt
     5101 acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt
     5161 cagtacagga cccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca
     5221 cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct
     5281 ggctcgatag cgaagagcgc cagttactac aacgtaaatg tagtctgctg gaaagctggg
     5341 ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata
     5401 atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg
     5461 acgaatttta tcgtaccgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc
     5521 cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc
     5581 tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact
     5641 ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtactga
     5701 aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag
     5761 atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggtctcgat ccatactgca
     5821 tgatgctgga gcgtgttact gaatacctga cggcgattga agattttacc cgtctggatt
     5881 tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaagct cagccgtgaa cgcgcctgcg
     5941 taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc
     6001 gtctggcaat gctcgataac cgcgctaact ggaagattga tcaggtgcgt gaggcgcaca
     6061 acgagttgct cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca
     6121 ataaccttag cgtctccgcc agtccgcagg atatcggcgt gctgacgcgt aagctgtatg
     6181 ccgcgtttga agcattacca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc
     6241 tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgtgctaac cgttcaggtt
     6301 ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat
     6361 ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc
     6421 gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg
     6481 tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct
     6541 acagcccgtg tgagatccgc catctggcga ttatcgttaa cctggaatat gacccgacag
     6601 cggcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg
     6661 gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg
     6721 aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc
     6781 tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc
     6841 agcatctgcg cgggttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac
     6901 tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc
     6961 aaacctgggg gttgttcttc gaacgcctga atgtatcggt acaaaaactg gaaaacgcca
     7021 tcgagtttta tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa
     7081 ccaatcacgt caaattaccg gcggtggtgg acggctttgc tagtgaaggg atcatccagt
     7141 tcttcttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca
     7201 accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggaactggtg cgtgacgtca
     7261 gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca
     7321 acctgccgca gttctatcag attgtgaagg ttgatggtcg tgaacaggtg attccgttcc
     7381 gcacaaaatc tatcggtaac atgccgcctg ccaatcagga tcacgatacg ccgctattac
     7441 agcaatattt ttcgtgatga acgtgccgga aagcgaggct tatccggcat gcaatcttag
     7501 cggaaactga ctgtttcacc cgcttgctgt gtcgccgcct gttccagcaa atcccagaag
     7561 gtttcgccgc tgcgatcgca aatccattca tcgcctttta ggtcaaaatg gtagccgccc
     7621 tgtttggttg ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg
     7681 ccgttttcaa aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg
     7741 ccatcccagt cgtccaggcg ttcttcaatg gtcagccaga gttgatcagc caggcgatga
     7801 aattcactgt cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat
     7861 ggggtcaggg cttcaaagtt tgcacctctg cggctgcgtt ccggcacgat tcatccgtca
     7921 ccggaataat gatgtctcag tgtagcgaaa gatttgtctc ttcattaggg cgcagttaca
     7981 ccacgccttt ccctgtttct ggtaaacatt atgatcaggt ttaccgagcg agcatcctca
     8041 cgctgacgga actaaaaaag ccaacaaaca aactacattg cgatagtgca taaagccatc
     8101 ctggcgcgag gtgccgatca cgaaactacc agcaaaacat aaatccccac gagtaagcgt
     8161 tatactcgca gcatttcctc acttttcaga cttcataaag agtcgctaaa cgcttgcttt
     8221 tacgtcttct cctgcgatga tagaaagcag aaagcgatga actttacagg caatccataa
     8281 tgaaaaacgt gtttaaggca ctcactgtat tacttactct cttcagcctg acgggctgcg
     8341 gtctgaaagg tccgctctat ttcccgcctg cagataaaaa cgcaccgccg ccgaccaaac
     8401 cggtagagac gcaaacgcaa tccacggtgc cggataaaaa cgatcgcgcc actggcgatg
     8461 gtccatccca ggtgaattac taaaagtcag tttctgtacc cgcgtgattg gagtaaatga
     8521 tgcagttctc gaaaatgcat ggccttggca acgattttat ggtcgtcgac gcggtaacgc
     8581 agaatgtctt tttttcaccg gagctgattc gtcgcctggc tgatcggcac ctgggagtag
     8641 ggtttgacca actgctggtg gttgagccgc cgtatgatcc tgaactggat tttcactatc
     8701 gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg caatggtgcg cgctgctttg
     8761 cccgttttgt gcgtctgaaa gggctgacca ataagcgcga tatccgcgtc agcaccgcca
     8821 acgggcggat ggttctgacc gtcaccgatg atgatctggt ccgcgtaaat atgggcgaac
     8881 ccaacttcga accttccgcc gtgccgtttc gcgctaacaa agcggaaaag acctatatta
     8941 tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc gatgggaaat ccgcattgcg
     9001 tgattcaggt cgatgatgtc gataccgcgg cggtagaaac gcttggtcct gttctggaaa
     9061 gccacgagcg ttttccggag cgagccaata tcggttttat gcaagtggtt aagcgcgagc
     9121 atattcgttt acgcgtttat gagcgtgggg caggagaaac ccaggcctgc ggcagcggcg
     9181 cgtgtgcggc ggtggcggta gggatccagc aaggtttgct ggccgaagaa gtacgcgtgg
     9241 aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc gggtcacccg ttatatatga
     9301 ctggcccggc ggtacatgtc tacgacggat ttattcatct atgaagcaac caggggaaga
     9361 actgcaggaa acactcacgg agcttgatga ccgggcggtt gtcgattatc tgattaaaaa
     9421 tcctgagttt tttatccgta atgcgcgcgc agtagaagcg atacgtgtgc cgcatccggt
     9481 acgcggcacc gtttcgttgg tcgaatggca catggcccgc gcacgtaatc atattcatgt
     9541 tctggaagag aacatggcgt tgttgatgga acaggctatc gccaacgaag gcctgtttta
     9601 tcgcttactc tacctgcagc gcagtctcac cgccgccagc agtctcgacg atatgctgat
     9661 gcgctttcac cgctgggcgc gcgatctcgg tctggcaggt gcgagtctgc gcctgtttcc
     9721 ggatcgctgg cgcttaggtg cgccgtcgaa ccacactcat ctggcattaa gccgtcagtc
     9781 tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag cactatcttg ggccgcttaa
     9841 cggaccagag ctgctggtgg tgctaccgga agcgaaagcg gtgggatcgg tggcgatgtc
     9901 gatgctggga agcgatgctg atttgggtgt cgtgctgttt accagtcgcg atgccagtca
     9961 ctatcaacaa gggcaaggaa cgcagttact tcatgaaatt gcgctgatgt tgccggagct
    10021 tctggagcgt tggattgaac gcgtatgacc gatttacaca ccgatgtaga acgctaccta
    10081 cgttatctga gcgtggagcg ccagcttagc ccgataaccc tgcttaacta ccagcgtcag
    10141 cttgaggcga tcatcaattt tgccagcgaa aacggcctgc aaagctggca gcaatgtgat
    10201 gtgacgatgg tgcgcaattt tgctgtacgc agtcgccgta aagggctggg agcagcaagc
    10261 ctcgcgttac ggctttctgc gctacgtagc ttttttgact ggctggtcag ccagaacgaa
    10321 ctcaaagcta acccggcgaa aggtgtttcg gcaccgaaag cgccgcgtca tctgccgaaa
    10381 aatatcgacg tcgacgatat gaatcggctg ctggacattg atatcaatga tcccctcgct
    10441 gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg gtctgcgtct ttctgagctg
    10501 gtggggctgg atattaaaca cctcgacctg gagtctggtg aagtgtgggt tatggggaaa
    10561 ggcagcaaag agcgccgcct gccgattggt cgcaacgctg tggcgtggat tgagcactgg
    10621 cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc tttttctgtc gaaactgggc
    10681 aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg aatggggcat aaaacaaggg
    10741 ctgaataatc acgttcaccc gcataaatta cgtcactcgt tcgctacgca tatgctggag
    10801 tcgagcggcg atcttcgtgg tgtgcaggag ctgctgggtc atgccaacct ctccaccacg
    10861 caaatctata ctcatcttga ttttcaacac cttgcctcgg tgtacgatgc ggcgcatcca
    10921 cgcgccaaac gggggaaata atgcgttttt accggccttt ggggcgcatc tcggcgctca
    10981 cctttgacct ggatgatacc ctttacgata accgtccggt gattttgcgc accgagcgag
    11041 aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg cagcttccag aatgaagatc
    11101 tgcaacgcct gcgccaggcg gtacgggaag cggaacccga gatttatcac gacgtgacgc
    11161 gctggcgttt tcgttcgatt gaacaagcga tgctcgacgc cgggctgagt gccgaagaag
    11221 ccagtgcagg cgcacacgca gcaatgatta actttgccaa atggcgcagc cgaatcgacg
    11281 tcccgcagca aactcacgac accttaaaac agctggcgaa gaaatggccg ctggtggcga
    11341 tcaccaacgg taacgcccag ccggaactgt ttggtttggg ggattatttt gagtttgtgc
    11401 tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga tatgtacttt ttggctgcgg
    11461 aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg ggacgatctc accactgacg
    11521 tgggtggggc aattcgcagc ggaatgcagg cttgttggat cagaccggaa aatggcgatc
    11581 tgatgcaaac ctgggacagc cgtttactgc cgcatctgga aatttcccgg ttggcatctc
    11641 tgacctcgct gatataatca gcaaatctgt atatataccc agctttttgg cggagggcgt
    11701 tgcgcttctc cgcccaacct atttttacgc ggcggtgcca atggacgttt cttacctgct
    11761 cgacagcctt aatgacaaac agcgcgaagc ggtggccgcg ccacgcagca accttctggt
    11821 gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg catcgtatcg cctggctgat
    11881 gagcgtggaa aactgctcgc catactcgat tatggcggtg acgtttacca acaaagcggc
    11941 ggcggagatg cgtcatcgta tcgggcaact gatgggcacc agccagggcg gcatgtgggt
    12001 cggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg caccatatgg acgccaatct
    12061 gccgcaggat ttccagatcc tcgatagcga ggaccagctg cgcctgctta agcgtctgat
    12121 caaagccatg aacctcgacg agaagcagtg gccgccgcgc caggcaatgt ggtacatcaa
    12181 cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa agctacggta atccggtgga
    12241 gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg tgtgaccgcg cgggcctggt
    12301 ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg cttaacaagc cgcatatcct
    12361 acaacactac cgcgaacgtt ttaccaatat cctggtggac gaattccagg ataccaacaa
    12421 cattcagtac gcgtggatcc gcctgctggc gggcgacacc ggcaaagtga tgatcgtcgg
    12481 tgatgacgac cagtcaatct acggctggcg cggggcgcag gtggagaata ttcagcgttt
    12541 ccttaatgat ttccccggtg ccgaaactat tcgtctggag caaaactacc gctctaccag
    12601 caatattctg agcgccgcta acgccctgat tgaaaacaat aacgggcgtc tgggtaaaaa
    12661 actgtggacc gatggcgcgg acggtgagcc tatttccctc tattgcgctt ttaacgaact
    12721 cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg caggacaacg gcggggcgct
    12781 tgccgagtgc gccattctct accgcagtaa cgcccagtcg cgtgtactgg aagaagcact
    12841 gttgcaggcc agtatgccgt accgtattta cggcgggatg cgcttcttcg aacgccagga
    12901 aatcaaagac gcgctctcgt atctgcgtct gattgccaac cgtaacgacg acgcggcgtt
    12961 tgagcgcgtg gtgaatacgc caacgcgggg tattggtgac cggacgctgg acgtggtacg
    13021 tcagacatcg cgcgatcgcc agttaacact ctggcaggca tgtcgtgaac tgttgcagga
    13081 aaaagccctc gccgggcgag ctgccagcgc cttgcagcga tttatggaat taatcgatgc
    13141 cttagcgcaa gaaactgccg atatgccgct gcatgtacag actgaccggg taattaaaga
    13201 ctccggcctg cgcaccatgt acgagcagga gaagggcgaa aaaggccaga cgcgtatcga
    13261 aaacttagag gaactggtga cggcaacgcg ccagttcagc tacaacgaag aagatgaaga
    13321 tttaatgccg ctgcaggcat tcctctccca tgcggcgctg gaagcgggcg aggggcaggc
    13381 ggatacctgg caggatgcag tgcagttgat gacgctgcac tcggcgaaag gcctggagtt
    13441 cccgcaggtg tttattgtcg gtatggaaga gggtatgttc ccaagccaga tgtcgctgga
    13501 tgaaggcggg cgtctagaag aagaacgccg tctggcctac gttggtgtaa cccgcgcgat
    13561 gcagaaactg acgctgacct acgcggaaac ccgccgtctg tatggtaaag aggtttacca
    13621 tcgcccgtcg cgctttatcg gcgagctgcc ggaagagtgt gtggaagagg tgcgcctgcg
    13681 cgctacggta agtcgtccgg tcagccatca acggatgggg acgccgatgg tcgagaacga
    13741 cagcggctac aagctcggtc agcgtgtacg ccacgctaag tttggtgaag gcaccatcgt
    13801 caatatggaa ggcagcggcg agcatagccg tttgcaggtg gcattccagg gccagggtat
    13861 taaatggctg gtggcggcat acgcccggct ggagtcggtg taacgttgcc ggatgcggtg
    13921 ctgcgcacct tatttggcct aaaaaatcat tcatattcaa taaattgcaa cgtcatgtag
    13981 gccggatagg gcgtttacgc cgcatccggc atctgcgcca tcttcagtat ctgacacaaa
    14041 actatcgttt taacctttcc gctctacgga aatcatggtg acaaaaggat agcgttgcca
    14101 cgggattgcc ccgcctttca tatacatatg tgaaatcgtg ccatcaagat aaagcagctg
    14161 ctcaacgttc agtttcgctt tggcataaca ggcaaaatca taaaaatttg tcgcctgctg
    14221 gctcaacaaa aacacggcat tcccatgttt attaatccca acaccgttac gaattttacg
    14281 tgaggcgacg ttgggatgaa tacgcggatt aattacactg ttttccatca acattggccc
    14341 tgactgcacc gcaaactgaa tctctttact ggttttgaag gcatccagac gaacgatgcc
    14401 gactttatct cccgcgacat aaaacacgcc gccaggacgg ataaagaaat tcccttcacc
    14461 tgaagcgaga tttaacgcca ccttctgctg accgttttcg atgtacaaac cgagcggcgc
    14521 atagctttca tcatagatgc cgccgttcat cgccatctgc acctgaccct gactattcat
    14581 atccgccagc agagcatgta aagttcccca cgcttcgcca ttggcttttt gccagtacat
    14641 tttcacccgc tctgtttgag gattaacggt atacgcctgt acggtcagcg tcggatctga
    14701 gagtgcgcaa tcatcagcgg caacagcaaa caagggaagt aaggtgaggg tgagaaaaat
    14761 ccgtttgaga ttcaaggtga tcattccttt accaatgagt agccgatgcg ccattatagg
    14821 tcctggatgt tggatttttt tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa
    14881 gttcaactat tgttctgtgg tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa
    14941 catgcgcgca cgatcactct aagaggacat tcgccttgga cacacccagt agatactggc
    15001 tcactatcct gtcatccagg atcaactcct aaggctatcc ctttttgctg atagccttag
    15061 cggttgtcag cgacctcaat ttttcccgtc gcgctgagtc aggctgttta atggtctgaa
    15121 acccaatttg tttctgtgtg cccaccgaac tgtccgatat tttaagcatt gggagtcccg
    15181 gtcatgctga gcgcatttca actggaaaat aaccgactga cccggctgga agtcgaagag
    15241 tcacaacccc ttgtaaatgc agtatggatt gatcttgtcg aaccggacga cgacgagcga
    15301 ctgcgcgtac aatctgaact tggccagagc ctggcaaccc gcccggaact ggaagacatc
    15361 gaagcatcgg cacgtttctt tgaagacgac gacggcctgc atattcactc cttcttcttc
    15421 tttgaagatg cggaagatca cgccggtaac tccactgtgg catttaccat ccgtgatggt
    15481 cgtctgttta ctctgcgtga gcgtgaactg cctgcattcc gtctgtatcg tatgcgcgcc
    15541 cgtagccagt caatggtgga cggtaacgcc tacgagctgc tgttggatct gttcgaaacc
    15601 aaaatcgaac agctggcaga tgaaattgaa aatatctaca gcgatctgga gcagttgagc
    15661 cgggtaatta tggaagggca tcagggcgat gagtacgatg aggcgctctc cacgctggcg
    15721 gaactggaag atatcggctg gaaagttcgc ctgtgtctga tggataccca gcgtgcgctc
    15781 aacttcctgg tacgcaaggc gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc
    15841 ctgcgagata tcgaatccct gctgccgcat aacgaatccc tgttccagaa ggtgaacttc
    15901 ctgatgcagg cggcgatggg ctttatcaac atcgagcaga accgcatcat caaaatcttc
    15961 tcggtggtat ccgtggtgtt cctgccaccg acgctggtgg cttccagcta cgggatgaac
    16021 tttgaattta tgccagagtt gaagtggagt ttcggctacc caggcgcgat tatctttatg
    16081 atcctcgcgg gcctggcacc gtatctgtac tttaagcgga agaactggtt gtaatatgag
    16141 tgccggataa agctattttt ttatccggct tatttctaaa aattgtcttt tttaaagtca
    16201 tgcggattga aaactcgctc tattttattt tcttgtgtta attgatactc agtaaaatcg
    16261 ttaataccaa tatgagtgtt acgataaggg tattcattaa taaccattaa gtaatttttc
    16321 acgatgcaat tatcattgct cttaattgtc gggaaacagt agagatcttc atacggccct
    16381 tcgatatatt ttttatagtc agtgaagcca ttaccgatat caatgacata ccaggtatgc
    16441 caactacctt ctggtagggg atgttgattt ttaatttcat tctgatgctt cagataccaa
    16501 tttattttat cgctgtcagt ccatggcaga tgatcaacga ccagaaagac tgaattgcca
    16561 gcatcagagt atgtatacaa aatctgtaca ggacgcaatg
//
LOCUS       DAPHDX010000096        16599 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPHDX010000096 DAPHDX010000000
VERSION     DAPHDX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.097"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..181)
                     /locus_tag="RH058_003161"
     CDS             complement(29..181)
                     /locus_tag="RH058_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV3138716.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(446..1180)
                     /gene="cysH"
                     /locus_tag="RH058_003162"
     CDS             complement(446..1180)
                     /gene="cysH"
                     /locus_tag="RH058_003162"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3138717.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1254..2966)
                     /gene="cysI"
                     /locus_tag="RH058_003163"
     CDS             complement(1254..2966)
                     /gene="cysI"
                     /locus_tag="RH058_003163"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV3138718.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2966..4765)
                     /gene="cysJ"
                     /locus_tag="RH058_003164"
     CDS             complement(2966..4765)
                     /gene="cysJ"
                     /locus_tag="RH058_003164"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV3138719.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGELRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5084..5446
                     /gene="queD"
                     /locus_tag="RH058_003165"
     CDS             5084..5446
                     /gene="queD"
                     /locus_tag="RH058_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV3138720.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5524..6795
                     /locus_tag="RH058_003166"
     CDS             5524..6795
                     /locus_tag="RH058_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3138721.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6786..7046
                     /locus_tag="RH058_003167"
     CDS             6786..7046
                     /locus_tag="RH058_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV3138722.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7063..7638
                     /locus_tag="RH058_003168"
     CDS             7063..7638
                     /locus_tag="RH058_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV3138723.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7786..8646)
                     /locus_tag="RH058_003169"
     CDS             complement(7786..8646)
                     /locus_tag="RH058_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV3138724.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCILAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8643..9422)
                     /locus_tag="RH058_003170"
     CDS             complement(8643..9422)
                     /locus_tag="RH058_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV3138725.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9400..10809)
                     /locus_tag="RH058_003171"
     CDS             complement(9400..10809)
                     /locus_tag="RH058_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138726.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10831..12285)
                     /locus_tag="RH058_003172"
     CDS             complement(10831..12285)
                     /locus_tag="RH058_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV3138727.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12355..13140)
                     /locus_tag="RH058_003173"
     CDS             complement(12355..13140)
                     /locus_tag="RH058_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3138728.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13459..14736
                     /locus_tag="RH058_003174"
     CDS             13459..14736
                     /locus_tag="RH058_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138729.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFSVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14763..16241
                     /locus_tag="RH058_003175"
     CDS             14763..16241
                     /locus_tag="RH058_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3138730.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCHGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3957 a         4371 c         4178 g         4093 t
ORIGIN      
        1 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
       61 taaactccat accacgttct ctgatactca actcacacaa cgagtcacct accatcagcg
      121 tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat tttgtcagca
      181 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc
      241 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
      301 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
      361 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag
      421 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
      481 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
      541 caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca
      601 ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
      661 gctgaattgc cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg cgcaggccag
      721 caaaccacgt ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga
      781 tgtcattgta cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt
      841 cctgccaggc ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg
      901 ttaactcgtc aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca
      961 ccggaatatc cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     1021 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt
     1081 cagcgtccag tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct
     1141 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     1201 tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc
     1261 cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg
     1321 ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac
     1381 gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg
     1441 cggttgccgc caagatgcag attgtagcga cccggtgctt tacccaccag gcccacttcc
     1501 gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca
     1561 tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa
     1621 cgctctgctt ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt
     1681 tcacgctgcg gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc
     1741 gctttctcgc tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa
     1801 tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa
     1861 tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta
     1921 acccagccaa tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg
     1981 atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg
     2041 tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg
     2101 acggcttcgg ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg
     2161 cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg
     2221 aagcctacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga
     2281 tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc
     2341 tgaccaagga tcggttcttc atcagtagtg gcgacttttt cctggtcgag ccagatctcc
     2401 gcatacgcgc gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct
     2461 tccgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg
     2521 ttagctgtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg
     2581 ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca
     2641 tagatggtgt tttcaccggc aaatttatcg atcgcttgcc actgtttggt ggtgatcacg
     2701 ccacccggca gacgacagcg caacagcatc gcatggcgcg gctccagctt ctgttcagca
     2761 cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg
     2821 aagttatcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg
     2881 cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagtttacc ttcgaccact
     2941 aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc
     3001 gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca
     3061 cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcaccg cagacataaa
     3121 tatgggcacc gtcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt
     3181 gtacgtaaac tttttctttt tggtcgcgcg accaggcgag atcgatccgc gtcagcacac
     3241 cctctttgac gtagcgctgc cactccacct ggtagaggaa atcttccgta aagtgcgggt
     3301 tgccaaagaa cagccagttt ttacccggtg cttcgtcggc agcacgttgc tgcataaagg
     3361 cgcggaacgg cgcgatgccg gtgcctgggc caatcataat caccggggtt tccgggttag
     3421 cgggcaggcg gaagttatcg ttatgttcga taaatacgcg gagttcgccc tcttcttcca
     3481 cgcggtccgc gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac
     3541 gcaccacacc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg
     3601 aatacagacg cggcgtcagc gggcgcagca gattaattag cgcttcggca tcaagctgtg
     3661 ccggggagaa acgcaccatg tcaacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg
     3721 ctttatcgcc caccagcggc agcagtgttt cactgcgggt aagcgtggcg taattctcaa
     3781 caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca
     3841 acgttttgcc ctcgacggtg acaggttcat cgcctgtcag ccacagcagt tcgacaagtt
     3901 ctttcaccag tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac
     3961 gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc
     4021 cggtaatttt ctggttaaca gagaggctag ccaccagtgg cgcgtctttg ctgtacgggc
     4081 tggtgtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg
     4141 cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt
     4201 caacatcggc atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc agcttgctgt
     4261 cgaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg
     4321 caaacgcggt gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga
     4381 cggcttcttc cggcggttcc ccttcccctt gcgttgacgt caccacgata agcagttttt
     4441 cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg
     4501 ccgtcaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgaag
     4561 cagagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag
     4621 caggctgctg attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag
     4681 ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg
     4741 cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg
     4801 cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggca aatggtgtgg
     4861 attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc
     4921 tttaacaaca aaatagatta accaacccaa tgaaaaacaa atgaatttag ccaatcatta
     4981 agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt
     5041 ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt
     5101 taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg
     5161 tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc
     5221 gcatacgggc tggattatcg atttcgctga actaaaagcg gcgtttaaac caacctacga
     5281 gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaggt
     5341 tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat
     5401 ggtaaaagaa acctgtaccg caggttgtat ctatcgcggc gaatgataag agtgtgtcgg
     5461 cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat
     5521 gacatggaag acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc
     5581 gcgttacgct gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc
     5641 ggcagcaaaa atctttccgg cgggcggtta tatacccatg cactcgcgga actcctcccg
     5701 caatttcatc tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta
     5761 acgcccgatg gcgtaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta
     5821 ttacgtgcac ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc
     5881 atccccggag cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt
     5941 tgtggtgacg atattctccg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc
     6001 ctggctgaac gtcacggatt agtgactcgt cctgctggcg aagcgatggc gttggggatc
     6061 aaagaagtgc tgtcgctgga aacatccgct attgaagaac gttttcatct ggagaataac
     6121 gaaggcgcag cgttgctgtt cagcggcagg atctgtgatg acttacccgg cggcgcattt
     6181 ctttatacta atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg
     6241 caaagtcgtg ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc
     6301 ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc
     6361 ttgcacagta tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg
     6421 cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgcg
     6481 caggcggcgg cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt
     6541 gcgctttatc atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag
     6601 catgttccgg cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag
     6661 gatatttccc gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta
     6721 ttctggcgtc atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt
     6781 ctgcgatgtc tgtagcccat aatctctggc gcgttgctga tgcgccgcac attgttccgg
     6841 ctgactccgt tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gcaggtcttt
     6901 tttcgctcac accggaaggt gacttacgcg ttgattatca cggctgcctg gagtgtggca
     6961 cctgccgttt gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat
     7021 tcggcatcac ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg
     7081 ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca actggcaatc
     7141 gactctgaat gccagtttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc
     7201 gttaaaaaga ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc
     7261 gcgtcaaata aagaagtcgt gattcagttt ttgaaactgg tgaccgaggc ggacggcatt
     7321 atcagtacca aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat
     7381 cgcctgttta ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg
     7441 aatccggatt gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca
     7501 gagaaaatcc gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt
     7561 aatgcgatta acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta
     7621 gcgaaaaaat tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc
     7681 cgcacgccac atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta
     7741 caagtcccca ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc
     7801 gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc
     7861 tgcctgtgaa aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt
     7921 gcgtacgccc gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg
     7981 tgccagcaga tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac
     8041 tcgcgcccga ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg
     8101 gttatctgcc tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc
     8161 gcgagtaaca tttttcagat cctctgtgct aaccagccag tcgggaagcg cacccgggac
     8221 aatgttcagt tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg
     8281 cgccagggaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc
     8341 ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct
     8401 tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg
     8461 aaagagaaca acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg
     8521 ttccgccact acaggctgag gttcaatctg ccagtgcgcc aggatacaac cgctgaaatc
     8581 ctgcgccgcc agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat
     8641 attcatggct gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct
     8701 accgtttgcc catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga
     8761 cactgcatcg ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca
     8821 gccattcgct ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca
     8881 atcaccgcag gcaagcgaac ccggcaacaa cgcaaaccat gttcagtacg ctgttcgagg
     8941 gtgataaaca gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc
     9001 atttccgcca gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta
     9061 attatcagat ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc
     9121 ggcgcaaagc gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc
     9181 atgagatagc gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac
     9241 atcggcgtac cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca
     9301 ccgagtaaac ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc
     9361 cactcttttt ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc
     9421 attcgcacct cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca
     9481 tagccaggtc acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg
     9541 cattccccac tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg
     9601 actcatggcg gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc
     9661 aggcaaaatg cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag
     9721 cagcagcgtt aatgaactgc cggaaggcaa acaggccatg actaccagcg ttgccgccag
     9781 cagcaaaaaa cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc
     9841 cagcagcgcg cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc
     9901 cagaccaata gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac
     9961 gaggcagaca aagaagacgc tgttaaacgc cgttcgccgc cagtaacgcg aagagaacaa
    10021 ggttttgatg tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc
    10081 aaaatagcga tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg
    10141 tggtgattct ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga
    10201 tgccagtagc cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact
    10261 tgccagcaca tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg
    10321 gcggcgcggg gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagacc
    10381 aatgccaatc aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa
    10441 aaacgaagca agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg
    10501 gtcggagatc cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg ccgagccgcc
    10561 gatcattccc gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta
    10621 accaatgacg ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat
    10681 gcggcagtga aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt
    10741 taccccttca ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg
    10801 ccccgtcatc gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt
    10861 attcataatg ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct
    10921 gccgtgttcc agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat
    10981 cgaaccgccg aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt
    11041 gtcgatttcc tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt
    11101 ggtgccgttc tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg
    11161 cgggaactca gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca
    11221 gcagccggac acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc
    11281 ggcagccact ttatccggtc cccagttcag gttgttaaac caggtttcga tcagcttgct
    11341 gtccacgcgc tggcattgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc
    11401 cttcgcaatg cgagggttac cttcagccat aaagatcagc acgcattttc cgtctgcaaa
    11461 atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata
    11521 cccttccacc atgacttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata
    11581 gccgtagaag aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat
    11641 atagcaaagt gcaccttcgt taccgatgat gatgtggcga atgtccgggc cagccgcgcg
    11701 gcgtggcacg tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac
    11761 gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac
    11821 caggccgccc atctgagcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt
    11881 ttcacgcaac gcattttcca gcacctccag cggaacgcca cattgcgccg tcgcctgcat
    11941 attctcaata tcaatattaa tgatttgatt catggcggag ccgtcgagca ccaccgagtt
    12001 ttccacaacg gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt
    12061 aattttgtgc gcattcataa aattcagcac acgggagact tgttctgtgg aaccgagttt
    12121 tacgaccgct gccggaatcg gcaaagtata aataccatga atatccggaa atttacgaaa
    12181 acggtcaata ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac
    12241 aatttccttt agctggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg
    12301 ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta tggattagcg
    12361 cactaaataa ccaccatcga ccactaataa atgcccattg acataatttg aagccggact
    12421 tgcgaggaat acagctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat
    12481 atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagcg taatatctgt
    12541 tgcataatag ccaggggcga taccatttac ctgaatatta tattgaccta gctcatcaca
    12601 ataagctttg gtgaacccgg caagagcatg tttagtggca gaatatgcag gtgaccattg
    12661 tccacctaag taagagaaca atgaacagat attaataatt ttgccgcttt tctgcgggat
    12721 cataattttt gcagcttcat agcttaactc gaatgcggcg gtcaggttca catcaatcat
    12781 cggatcccag tcggcacgac cgaagtccag caccttattc agcttacaaa taccggcatt
    12841 gtttaccaga atatcaactg taccgaaacg ctcacagcaa gcagcgataa tcttctgcgg
    12901 cgcgccttct gcggtgatat ccacctgcat gaagtccacc tcaacaccct gtttttcaat
    12961 catttccttt gtttcgccgt tatctttgac gaaactagga ataaagatat ttgcgccagc
    13021 tttggccaac gccatggcaa atgcctggcc taaaccgcta ttcccaccgg taacaattgc
    13081 ggttttacct ttcagtgaga aaaaatccat tgagaacgca ttgagagatt cgattgacat
    13141 agttagctcc aatttccgta aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga
    13201 agttaccttt ctcattatct ctggtaactg gagataattt ttagcataac ggcataactg
    13261 ctaatgtgat cgtaatcaca gtgtgatatt cgtattgtca aatagtttta aaaagcagaa
    13321 gcgtgattac tctcacacaa cacctctgct ttccatgtta tttcttattg caattactag
    13381 agacctgaga aaagtaatcc acctgaaagg gcggcttact tttctctttt ttttgtctat
    13441 tacaaatggg aatacgatat gcaacacaac tcatatcgcc gttggataac cctcgcgata
    13501 attagtttta gcggcggcgt tagtttcgac ctggcttatt tacgttatat ttatcaaatt
    13561 cccatggcga aatttatggg attcagcaat accgagatag gtttaataat gagtaccttt
    13621 ggtattgcgg ctattattct ttatgccccc agcggcgtta ttgccgataa attttcacac
    13681 cgcaaaatga ttacttccgc gatgatcatt accggattac tgggtctgtt aatggcaacg
    13741 tatccaccgc tgtgggtaat gctctgtatt caggtcgcct ttgcgataac gacgatttta
    13801 atgctgtggt cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag tgagcaaggg
    13861 aaaattatgg gctggatgga agggctgcgc ggcgtcggtg taatgtcgct ggcggtgttt
    13921 accatgtggg tcttttctcg ctttgcaccg gatgacagcg ccagcctgaa aacagtcatt
    13981 atcatctaca gtgtggttta catcttgttg gggattctgt gctggttttt tgttagcgat
    14041 aacaacaacc tgcgcagtgc caataacgaa gaaaaacagt cattccagct tagcgacatc
    14101 cttgccgttt tgcgtatcag caccacctgg tattgcagca tggtgatttt tagtgtcttc
    14161 acaatctacg ccattctgag ttactccacc aactatctga ccgaaatgta cggcatgtcg
    14221 ctggtggcag cgagctacat ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc
    14281 ccacttggcg gcattatcac cacctacagt aaagtgaaat cccctacccg cgtgatccaa
    14341 atcctttccg tactcggcct gctggcgtta actgccctgc tcgtcacgaa ctctaacccg
    14401 caatcggtcg cgatggggat tggcctgatt ttactgctgg gattcacctg ttacgcctca
    14461 cgcgggctgt actgggcctg ccctggcgaa gcgagaacac cgtcttacat tatgggcacc
    14521 acggtaggta tttgttcggt gattggattc ctgccggatg tcttcgttta cccgattatc
    14581 ggccactggc aagacaccct gcccgcagca gaagcctacc gcaatatgtg gctgatggga
    14641 atggcggcgc ttgccatggt gattgtcttt acctttttgc tgttccaaaa aattcgtact
    14701 gctgatagcg cccccgcaat ggctagcagc aagtaagcca ggaaaattac aggagaaaaa
    14761 tcatgtcgaa aaaatacatc atagggattg atggcggaag tcagagcaca aaagtggtga
    14821 tgtacgatct ggaaggtaac gtggtttgcg aaggcaaagg cttattacag ccgatgcaca
    14881 cgccagatgc cgatactgca gaacatcctg acgacgattt atgggcatca ttatgttttg
    14941 caggtcacga tttgatgagc cagtttgccg ggaataaaga agatattgtt ggcatcggcc
    15001 tgggctccat ccgttgctgc cgtgcgttat tgaaagccga cggcacgcca gctgcgccgt
    15061 tgattagctg gcaggatgca cgcgttacac gcccttacga acatacgaat cctgacgtgg
    15121 cgtatgtcac ctctttttcg ggttatctga cgcatcgctt aaccggcgag tttaaagaca
    15181 atatcgccaa ctattttggt cagtggccgg tggattataa gagctgggca tggagcgaag
    15241 atgctgcggt aatggataag tttaatatcc cccgtcatat gctgtttgat gtgcaaatgc
    15301 ctggcaccgt cctcggacat atcacaccac aagccgcact ggcgacacat ttcccggcag
    15361 gactgccggt tgtttgtacc accagtgata aaccggtaga agctctgggg gccggattac
    15421 tggatgatga aactgcggta atttctcttg gcacttacat cgcattgatg atgaacggca
    15481 aagcactgcc gaaagatccg gtggcgtact ggccgattat gtcttctatt ccgcaaacat
    15541 tgctgtatga aggttacggt attcgcaaag gtatgtggac ggtgagctgg ctgcgtgaca
    15601 tgttaggcga gtcgttaatt caggatgcca gggcgcagga tctttcaccg gaagatttac
    15661 tcaacaagaa agcctctagt gtgccacctg gctgtcatgg gctgatgacg gtgctggact
    15721 ggctgaccaa tccgtgggaa ccgtacaaac gcgggattat gatcggcttt gattccagca
    15781 tggattacgc atggatatat cgttcgatat tggaaagtgt ggcgctaacg ctgaagaaca
    15841 attacgacaa tatgtgtaat gaaatgaatc actttgcgaa gcatgtgatc attactggcg
    15901 gcggttcgaa cagcgatctg tttatgcaaa tttttgccga cgtgttcaac cttccggcac
    15961 gccgtaacgc cattaacggt tgtgcaagtc tgggagcagc gattaataca gcggtaggtc
    16021 tggggctata cccggattac gcaacggctg ttgataagat ggttcgcgtg aaagatatct
    16081 ttataccgat tgagagcaat gccaaacgct acgacgcgat gaataaaggc attttcaaag
    16141 acctaaccaa acatactgat gtgatcctga aaaaatcgta tgaagtgatg catggggaat
    16201 tggggaatgt ggattcgatc cagagctggt cgaatgcgta agtgaggaag gccgggcggg
    16261 aaactgcccg gcctgaacat acctgaatgg ttatccccgc tgacgcgggg aacataagtt
    16321 cgacaactta aaaaatgata acaagtccgg tttatccccg ctgatgcagg gaacataata
    16381 aaaactttaa ctagttcggt ttatccctgt ttttacaagg aatattaact acactcgatg
    16441 tctttagaat aaaagacaat acgaatcgca ctttatcaat tttcacttaa agtatgaact
    16501 ggatatcttt attcataaga actattcatc aacagcgtat agaggcggtt atgtaaaacc
    16561 actcattagc ctcaaaacaa agacaaactt ccagattta
//
LOCUS       DAPHDX010000097        16480 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPHDX010000097 DAPHDX010000000
VERSION     DAPHDX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.098"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(160..2121)
                     /gene="topB"
                     /locus_tag="RH058_003176"
     CDS             complement(160..2121)
                     /gene="topB"
                     /locus_tag="RH058_003176"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV3138731.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2126..3169)
                     /gene="selD"
                     /locus_tag="RH058_003177"
     CDS             complement(2126..3169)
                     /gene="selD"
                     /locus_tag="RH058_003177"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV3138732.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3286..3837)
                     /locus_tag="RH058_003178"
     CDS             complement(3286..3837)
                     /locus_tag="RH058_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV3138733.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3998..5854
                     /gene="sppA"
                     /locus_tag="RH058_003179"
     CDS             3998..5854
                     /gene="sppA"
                     /locus_tag="RH058_003179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV3138734.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRLIPAQQVFPGAQGLLVGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTIENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6021..7037
                     /gene="ansA"
                     /locus_tag="RH058_003180"
     CDS             6021..7037
                     /gene="ansA"
                     /locus_tag="RH058_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV3138735.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7048..7689
                     /gene="pncA"
                     /locus_tag="RH058_003181"
     CDS             7048..7689
                     /gene="pncA"
                     /locus_tag="RH058_003181"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV3138736.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7850..8122)
                     /locus_tag="RH058_003182"
     CDS             complement(7850..8122)
                     /locus_tag="RH058_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV3138737.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8164..8577)
                     /gene="msrB"
                     /locus_tag="RH058_003183"
     CDS             complement(8164..8577)
                     /gene="msrB"
                     /locus_tag="RH058_003183"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV3138738.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8919..9914
                     /gene="gapA"
                     /locus_tag="RH058_003184"
     CDS             8919..9914
                     /gene="gapA"
                     /locus_tag="RH058_003184"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV3138739.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9998..10882
                     /locus_tag="RH058_003185"
     CDS             9998..10882
                     /locus_tag="RH058_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV3138740.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10933..11788)
                     /gene="yeaE"
                     /locus_tag="RH058_003186"
                     /pseudo
     CDS             complement(10933..11788)
                     /gene="yeaE"
                     /locus_tag="RH058_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
     gene            complement(11878..12624)
                     /gene="mipA"
                     /locus_tag="RH058_003187"
     CDS             complement(11878..12624)
                     /gene="mipA"
                     /locus_tag="RH058_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV3138741.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13060..14994
                     /gene="yeaG"
                     /locus_tag="RH058_003188"
     CDS             13060..14994
                     /gene="yeaG"
                     /locus_tag="RH058_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV3138742.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15107..16390
                     /locus_tag="RH058_003189"
     CDS             15107..16390
                     /locus_tag="RH058_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV3138743.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4052 a         4261 c         4065 g         4102 t
ORIGIN      
        1 ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac atactttaga
       61 ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc cgctggatat
      121 ttcagaatat gactcgaata gcacgaaaga ttcactcgct tacgctatcg ccccgcttcc
      181 gacttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag cctttttctt
      241 atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca ccggcgtacg
      301 tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa agtcctgata
      361 gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg
      421 tcgcgtcgcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg tagagtggat
      481 atagcgccct tttttggtca gaaaaccacg cttgaacagc agttcaataa tcccggcacg
      541 ggttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat ctttgtcctg
      601 cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt gcatcggtaa aatggcgcgg
      661 cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat cgcctttcgc
      721 caccacaggc aatggcgtac cgtcgttttc ttcatcgcgc tctttgctgc ctaacagcgt
      781 gcgccagcct gcttcagcaa gaaaacgggc tttagcgaca aatttgcctt tggcaatgtc
      841 cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca ggtattgacg
      901 ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag aactccgtgc
      961 ggtcgggatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc ggttgcgtat
     1021 atctggatct accactggct gcggcaacag atctggtgca tgaacgctga tggcattcat
     1081 caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg aacgcggata
     1141 agtgattagc ttgtgcgttt cgtacagctt ctggcagata tcaagcacgt tctgcgcgct
     1201 cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg gcagcggcgc
     1261 ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttgac cgctgatgcg
     1321 gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat cctggtacgg
     1381 ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat
     1441 atgtgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt catcgcggcg
     1501 caccaccagc ccaagcacgg gcgtctgcac gcgtccgacg gaaagcacgc cctgataacc
     1561 ggcattgcga ccgagaatgg tgtaagcacg ggtcatattg atgccgtaca gccagtcggc
     1621 acgcgctcgc gccagcgcag aaacgcacag cggtacaaac tcactgttgg aacgaagacg
     1681 gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac gctgcacctg
     1741 ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca gcaattgccc
     1801 ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga agcgtttgat
     1861 gacgttaagt tgtttggtca cggagggtcg tggctgtaat tgccactttt ccgggacaat
     1921 cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg
     1981 ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact cgataaagcc
     2041 atcgccttta cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca gactcggttt
     2101 ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga ccgccgcgcg
     2161 ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct gtagttttaa
     2221 cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt tgcggatcgc
     2281 acagcagatc gcgcacttca cgcggcattt cacccatcag atgaccgtag ctggcaaagt
     2341 tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc cccggcaatt
     2401 ttgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac atttcgctca
     2461 aatggcccag cagaccaaaa ccagtaacgt cggtcatcgc ttttacgcct tcgatgttgg
     2521 caaaggacgc acctgcgatg ttcatccggc acatcacttc cgtcgccagt ccctgatgtt
     2581 ctggtttcaa cagtgatttt ttctcagccg tggtaagaac gccgatcccc agcggtttcg
     2641 tcaggaacag tttgcatccg gcttgtgcgg tactgttttt cttcacccgc tcggtcggta
     2701 cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag tgaccgccag
     2761 ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact tcgcgggcaa
     2821 tttctgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata atcggtttgc
     2881 cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca aaatcgaaag
     2941 gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg ccattgccca
     3001 gatcgtacac cgccgcatcg tcgcgggtct cattacccac aagcaaattc ggatcaacaa
     3061 acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt ttacagccgc
     3121 aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg gacatctcct
     3181 gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga gagtgtctga
     3241 attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga aataagttac
     3301 aaacggagtc gggtccggga cgttaatcga cgtagatgct ttcaactgcg gcgtgccgag
     3361 gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac gcactaccgg
     3421 actttcagtt aatgcgccac tgcgccagat gccgccaaat ccttgggcaa cggctgccat
     3481 ttgcatcgcc atgaccgcgc atccggcaga catttcctgt tcccatcgcg ggactttatg
     3541 attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg gcgcattacg
     3601 ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt ccagtacggc
     3661 gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt gcatggactt
     3721 atggtccggc gcacgcatac ccgcacgcag gatgttttgc agttgttcac ccgttggcgc
     3781 gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg catccatttg
     3841 attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt ttgttacagc
     3901 acagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc acatctattg
     3961 cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt ggcgatttat
     4021 tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa tggtacttaa
     4081 cctgttcttt attttcctcg tactggttgg tgtggggatc tggatgcagg tcagtggtgg
     4141 tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg gacatttctg gtgtgatcgt
     4201 cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc ttggtgccag
     4261 ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc gccaggcgaa
     4321 ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag gcggcgacca
     4381 gccgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg ggaaaccggt
     4441 ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt tcgccaataa
     4501 aatttggctg tcaccgcaag gcgtggttga tctgcacggt tttgctacca acggtctgta
     4561 ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg tgggtacgta
     4621 taaatctgcc gttgaaccgt ttattcgtga tgatatgtca ccggcagcgc gcgaagctga
     4681 cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg ctaaccggct
     4741 gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgtgggtt taaccaaaac
     4801 cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac tggcatcgag
     4861 tgccgaaatc gaaaaagctc tgaccaaaga gttcggctgg agtaagactg ataaaaatta
     4921 tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg gtgacagcat
     4981 cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg ggaatgttgg
     5041 cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag tgaaagcgat
     5101 tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga ttcgcgctga
     5161 actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg gcatggcggc
     5221 atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc ccagcaccct
     5281 gaccggttct atcggtatct tcggcgtgat caccaccata gaaaatagtc tggattcgat
     5341 tggtgttcat accgatggtg tctcaacttc accgctggca gatgtttcta tcaccagggc
     5401 actgccgccg gaagcacagc agatgatgca gttaagcatt gagaatggct ataaacgctt
     5461 tatcacgctg gttgctgatg cgcgtcattc gacgccggag caaattgata aaatcgccca
     5521 gggccacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata gtctcgggga
     5581 tttcgatgat gcggttgcca aagcagcaga gctggcaaaa gtgaaacagt ggcatctgga
     5641 atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt ctggttctgt
     5701 ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgcttg cctcggtagc
     5761 ctctaccgtt aaaagtgaaa gcgacaagct ggccgcgttt aacgacccac aaaaccgtta
     5821 tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactgagtgg ccgacagatc
     5881 gtcggccaca ttatttttta cctcgacgaa tcctcttccc gctgtctcga cccatatttc
     5941 cttatccgca tagtatcagg tgcgctcctc ctgcctcacg tatatacttt tgctctttcg
     6001 atatcattca tatcaatatc atgcaaaaga aatcaattta cgtcgcctac acgggcggga
     6061 ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat ctacaacgcc
     6121 aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc attcatgaat
     6181 atacgccgtt gatggattct tctgatatga cgccggaaga ctggcagcat attgctgaag
     6241 atattaaagc gcactatgac gactatgatg gttttgtcat tctacacggt accgacacga
     6301 tggcgtatac cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa ccggtcattg
     6361 tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt aatctgctga
     6421 atgcgttgta cgttgcggcg aattatccga tcaacgaagt aacgctcttt ttcaataacc
     6481 gattgtatcg cggcaaccgc actaccaaag cccatgccga tggttttgat gcgtttgcct
     6541 ctccaaacct tcctccgtta ctggaagctg gtatccatat tcgtcgtttg aatacgccac
     6601 ccgccccgca cggtgaaggg gaattaatcg ttcatccaat cacgccacaa ccaattggcg
     6661 tagtgacgat ttatccgggg atttctgctg atgtcgtgcg caattttctg cgccaaccgg
     6721 tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc gccacaaaac aaagccttcc
     6781 tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg acacaatgta
     6841 tgtccggtaa agtgaatatg ggtggttatg ccaccggtaa cgccctcgcc catgccggcg
     6901 ttattggcgg tgcagatatg actgtagaag ccacgctaac caaactgcat tacctgctga
     6961 gccaggaact ggatactgaa accattcgca aggccatgag ccaaaacctg cgcggcgaac
     7021 tgacgccgga tgattaagga gactgcaatg ccccctcgcg ccctgttact ggtcgattta
     7081 caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg aaggtgacag tacggtggat
     7141 gtcgctaacc gccttattga ctggtgccag tcgcgcggtg aagcggttat cgccagtcag
     7201 gactggcacc cggcgaatca cggcagtttt gccagtcagc actgtgtaga gccttatatg
     7261 ccaggtcaac tcgacggttt gccacaaacc ttctggccag atcactgtgt gcagaacagt
     7321 gaaggcgcac aattacatcc gttactgaac caaaaagcga tcgcagcggt gttccataaa
     7381 ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggccg tcggcagaaa
     7441 acctctctcg atgactggtt acgcgatcat gaaatcgatg aattgatcgt tatgggcctg
     7501 gctactgact attgcgtgaa gtttaccgtg ctggacgcgt tacagttagg ttataaggta
     7561 aacgtgatta ccgatggttg tcgtggcgtg aatatccagc cccaggacag tgcgcacgcg
     7621 tttatggaga tgtcagcagc tggggcaacg ctatatacgc tggcagactg ggaagagacg
     7681 caggggtaat attagttgaa ttcatctctg gcctacattt cctcaatgac attgtaggcc
     7741 agataatact tgtcagtgtt gcatttaacc tacttgcagc taaaacacga cgtcctaaca
     7801 agtatatttg ccctccctta ataacaggct taaatactag ataactcatt cacttaaaca
     7861 tagcaatagg ctttgctgag ataccaaaat cttcttttaa ctgctgtttg ctcttcatga
     7921 ccatctgacc atttgtgtca attgtcatat gctgtgcttc ggtattatgg cgggcttgcc
     7981 acagcatcac cagttgcagg caattctctt tttgttcctc ggtcaacgca acgccatcag
     8041 gccatttccc cagttcaacg gcggtcgaca aacgctggta tacctcaggc gtcatgctgt
     8101 tgataatgtc atcaagattc ataatttttc cgctcctgtg gaataagttg ctgaatcgtt
     8161 ttttcaaccg ttgatttctt caccgttttc gccatcagtg aagcgtaaag aagcagagtt
     8221 aacacaataa cgttcgcctg ttggttgcgg cccgtcggga aagacatgcc ccagatgggc
     8281 atcacagtta ccgcaacgaa tttctatgcg ctgcattcca tgtgacaagt ctttgatata
     8341 acgaatggat tcttcactca ccggttcgta gaaactgggc cagccacagc cggaatcata
     8401 cttcgtttcg gaatgaaaca gcggggcatc gcagatcaaa cagtgatata cgccgtcacg
     8461 cttgttatgc agtaaacgac ccgtaaatgg cggttctgtc ccatgattct gcgtcacgta
     8521 aaactgcatc tcggacaaat tttttttcag ttcttctgcc gaaggtttat tagccatttg
     8581 ctcacatctc actttaatcg tgctcacatt acgtgactga ttctaacaaa acattaacac
     8641 caactggcaa aattttgtcc taaacttgat ctcgacgaaa tggctgcacc taaatcgtga
     8701 tgaaaatcac atttttatcg taattgccct ttaaaattcg gggcgccgac cccatgtggt
     8761 ctcaagccca aaggaagagt gaggcgagtc agtcgcgtaa tgcttaggca caggattgat
     8821 ttgtcgcaat gattgacacg attccgcttg acgctgcgta aggtttttgt aattttacag
     8881 gcaacctttt attcactaac aaatagctgg tggaatatat gactatcaaa gtaggtatca
     8941 acggttttgg ccgtatcggt cgcattgttt tccgtgctgc tcagaaacgt tctgacatcg
     9001 agatcgttgc aatcaacgac ctgttagacg ctgattacat ggcatacatg ctgaaatatg
     9061 actccactca cggccgtttc gacggtaccg ttgaagtgaa agacggtcat ctgatcgtta
     9121 acggtaaaaa aatccgtgtt accgctgaac gtgatccggc taacctgaaa tgggacgaag
     9181 ttggtgttga cgttgtcgct gaagcaactg gtctgttcct gactgacgaa actgctcgta
     9241 aacacatcac cgctggtgcg aagaaagtgg ttatgactgg tccgtctaaa gacaacactc
     9301 cgatgttcgt taaaggcgct aacttcgaca aatatgctgg ccaggacatc gtttccaacg
     9361 cttcctgcac caccaactgc ttggctccgc tggctaaagt tatcaacgat aacttcggca
     9421 tcatcgaagg tctgatgacc accgttcacg ctactaccgc tactcagaaa accgttgatg
     9481 gcccgtctca caaagactgg cgcggcggcc gcggcgcttc ccagaacatc atcccatcct
     9541 ctaccggtgc tgctaaagct gtaggtaaag tactgccaga actgaatggc aaactgactg
     9601 gtatggcgtt ccgcgttccg accccgaacg tatccgtagt tgacctgacc gttcgtctgg
     9661 aaaaagctgc aacttacgag cagatcaaag ctgccgttaa agctgctgct gaaggcgaaa
     9721 tgaaaggcgt tctgggctac accgaagatg acgtagtatc taccgatttc aacggcgaag
     9781 tttgcacttc cgtgttcgat gctaaagctg gtatcgctct gaacgacaac ttcgtgaaac
     9841 tggtatcctg gtacgacaac gaaaccggtt actccaacaa agttctggac ctgatcgctc
     9901 acatctccaa ataagttgag atgacactgt gatctaaaaa gagcgacttc ggtcgctctt
     9961 ttttttacct gataaaatga agttaaagga ctgcgtcatg attaagaaaa tttttgccct
    10021 tccggtcatc gaacaaatct cccctgtcct ctcccgtcgt aaactggatg aactggacct
    10081 cattgtggtc gatcatcccc aggtaaaagc ctcttttgca ttacagggcg cacaccttct
    10141 ctcgtggaaa cctgcgggtg aagaagaagt tctgtggttg agcaacaaca caccgttcaa
    10201 aaatggcgtc gctattcgcg gtggcgtacc ggtttgctgg ccgtggtttg gtccggcggc
    10261 acaacaaggt ctgcctgcgc acggttttgc ccgcaacctg ccgtggacgc tgaaatcaca
    10321 tcgtgaagat gctgatggcg tagcgctgac ttttgaattg acgcaaagcg aagagacgaa
    10381 aaaattctgg ccgcacgact ttacgctgtt agcgcatttc cgcgtgggta aaacctgtga
    10441 aatcgatctt gaatcacatg gcgaatttga aaccacctct gccctgcata cctactttaa
    10501 cgtgggtgat atcgctaagg taagcgtcag tgggctgggc gatcgcttca ttgataaagt
    10561 aaatgacgcg aaagaagatg tactgaccga cggtattcag accttccctg accgtaccga
    10621 tcgcgtgtat ctgaatccgc aagattgcag cgtgattaat gatgaagcgc tgaatcgtat
    10681 tatcgccgta ggccaccagc atcatctgaa cgttgtcggc tggaacccgg gaccggcgct
    10741 ttcagttagc atgggcgaca tgccggatga tggctacaaa acatttgttt gtgtagaaac
    10801 ggcttacgct tcagaaacgc aaaaagtgac caaagagaaa cctgcacatc tggcgcaatc
    10861 cattcgcgtt gcgaaacgtt aatttgcgtt aatgttgtgt gccgggtgcg atgcatccgg
    10921 cacgtatcaa catcacacca tatccagcgc agtttttcct tttggtgccg gatacgcttt
    10981 atccagcatg gttaattccg ctgacgaaag ttcgacctca agcacagccg cattctgttg
    11041 gacatgggca atcgtggccg cttttggaat cgccatcaca ccctgatggc tgattaccca
    11101 cgccaacaat acttgtgccg cgctgatatt gtgagcatgt gcaatttcgt tgactaccgc
    11161 gttttttaac agtccattgc gcaaccgccc ggcctgggct aatggactgt aagccatcac
    11221 cggcatctgc tgttgctggc accaggggag tagatcgtac tcaatccctc gtgaaccgag
    11281 atggtaaagc acctgattag tggcacactg atttcccccc ggcagctgcc agagttcctg
    11341 catatcagca tgatcaaggt tagaaacgcc ccagcggcgg atttttccct gggcgatcaa
    11401 tttttccatc gctgccacag tctcttcaaa agcgaaactg ccagaccagt gtaataagta
    11461 gagatcgaga taatcagtat tgagacggcg taaactggct tcgcatgcat ttatcgcttt
    11521 ttgcccgcca gcattccacg gatagacttt agagacgaga aagacgttat cgcgcagacc
    11581 ggttaatgct tccccaacca ccttttcggc accgccatcg gcatacattt cggcggtatc
    11641 aatgagggtt aaaccgagct caatgcccgc gcgtagtgca gcaacttctg ttttgcgctg
    11701 actggcatct tcgcccatat accatgttcc ctgcccttat ggctggcagt gagacatcgc
    11761 cactaaattg aatcattttt tgttgcattg tttcctccca ggtattgcac caccgtaatg
    11821 caaaacaggg cgtgacgccc tgtttttatg cacaaaatgc cctggaaaga tgcattatca
    11881 gaatttgtag gtgatcccgg tagaaatcag gccagtccag gatttatcca ccatcgggct
    11941 gtcagtaact tcatcagaca gacgggtata gcgcgcggta ccgtaaacac tccagtcgcc
    12001 gaggaagttg tagctggcgc tcagctccag gtaagggctc cagctgtcgt tcgggttata
    12061 gccacgcaga ccgctgcgag cggactcttt gcgcgatacg ccataatagt attcgttctg
    12121 gttttcgctg ttccactgca caccaatacc cggagtcacg gtcaggccac cgttggtgta
    12181 acgatacaac caggccatat cccagacaat gccgttgctg ttatccaggg tatcgccagc
    12241 cagggtggta cgcaggtaac cgtactgggt aaagtgagca taagacagac cagccatcat
    12301 ggtgctctta cggtcatcca ggtgacgcat ttggtgatcg ccactgtctt tcgctttgaa
    12361 gtaaagcggc gaccagtaag cggtaattga aagtttatcc gttgcgtcat tccacaggta
    12421 gtaaccacca cctaagccac ggaaccagaa gttatcgcct tcatagttga ttaccggtac
    12481 tgggtaaaca tcggtatcgt aatctttata tgggtgctca acgacaccta cgcctgcgcc
    12541 cagggaaaat ttaccttcag cgtgcgctac gcctgcagac gttgcgataa gcactccaag
    12601 tgccagaagt ttgagtttgg tcacaattaa tcattcctta aacaaatgtt tagcgggcga
    12661 caaagtttac ccgtcaatac atcgaaaccc aaccttttta cgttttcatt ttttaaagta
    12721 actgtttaat tttcctgacg cggatgacac cgcgcttaca gccaaatgaa tttagcgtta
    12781 ctggctaccc tggtctttac attaattatg caaaatttat ggatgagttg ttgatatgcc
    12841 attgaaatta agaaagccgt gcaggcaagt tttccatttg ccatctacgc ttaattttga
    12901 aggtgtatca ccgggcacgt tgttctcatc gtcgataaaa tggcatgaga gttgctgtgt
    12961 tttagcaaga gacgtcgttc agtttacctc ttccgggagc ctctactatt catatgaacg
    13021 gctcttaacc tgtgctaaaa aacgaaagga cggcatacca tgaatatatt cgatcactat
    13081 cgccagcgat atgaagctgc caaggacgaa gagttcacac tgcaggagtt tcttaccact
    13141 tgtcggcaag atcgcagtgc ttatgccaac gcggctgagc ggctattgat ggctatcggt
    13201 gagcctgtca tggtcgatac agcccaggaa cccagacttt ctcgactctt ttctaaccgg
    13261 gtcattgcac gttatccggc atttgaagag ttttacggca tggaagacgc gattgaacag
    13321 attgtctctt atctgaaaca cgcggctcag gggctggaag agaagaaaca aatcctgtat
    13381 ctgctggggc ctgtgggtgg gggtaaatca tcgcttgctg agcgactgaa atcattaatg
    13441 cagctcgtac cgatttatgt attgagcgcc aacggtgagc gtagcccggt caacgatcat
    13501 ccgttctgtc ttttcaatcc gcaggaagat gctcagattc tggaaaaaga gtatggcatt
    13561 cctcgccgtt atctcggcac catcatgtcg ccatgggcgg caaaacgcct gcatgaattt
    13621 ggtggcgata tcactaagtt ccgggtagtg aaggtctggc cgtcaattct gcaacaaatt
    13681 gctatcgcca aaacggaacc cggtgatgag aacaaccagg acatctccgc gctggttggg
    13741 aaagtcgata ttcgtaaact cgaacactac gcgcagaatg acccggacgc ctacggctat
    13801 tccggtgcgc tgtgccgcgc caaccaaggg atcatggaat ttgtcgagat gtttaaagca
    13861 ccgataaaag tgctgcatcc gctgttaacc gccactcagg aaggtaacta caacgggacg
    13921 gaaggtatct ccgccctgcc gttcaacggg attattctcg cccactcgaa cgaatccgaa
    13981 tgggtcactt tccgtaataa caaaaacaac gaagccttcc tcgaccgtgt ttacatcgtg
    14041 aaggtgccgt attgcttacg catttccgaa gagatcaaaa tctacgagaa attgcttaat
    14101 cacagtgaat tgactcacgc cccctgcgcc cctggcacgc tggaaacgct gtcacgtttt
    14161 tccattcttt cgcgccttaa agagccagaa aactccagca tttattcaaa gatgcgggtt
    14221 tatgatggcg aaagtctgaa agacaccgat cccaaagcca agtcgtatca ggaatatcgt
    14281 gactacgccg gtgtcgatga agggatgaac ggtctgtcga cgcgttttgc gtttaagatc
    14341 ctctcccgcg tgttcaactt cgatcatgta gaagtggcgg caaacccggt ccatctgttc
    14401 tacgtcctgg aacagcagat cgagcgcgag cagttcccac aagagcaggc agaacgctat
    14461 ctggagttcc tgaaaggtta tctgatcccg aaatatgccg agtttatcgg caaagagatc
    14521 cagacggcct accttgaatc ctattccgaa tatgggcaaa acattttcga ccgttatgtt
    14581 acctacgcgg atttctggat tcaggatcag gagtatcgcg atccggatac cgggcagctg
    14641 tttgaccgcg agtctcttaa cgccgagctg gagaaaatcg agaaaccggc ggggatcagt
    14701 aatccaaaag atttccgcaa cgagattgtt aacttcgtac tgcgcgccag agcgaataac
    14761 agcggacgca atccgaactg gaccagctat gaaaaactgc gcacggttat cgagaagaaa
    14821 atgttctcca ataccgagga gctgttgccg gttatctcgt ttaacgccaa aacgtcaacc
    14881 gacgagcaga agaaacacga cgactttgtc gaccgtatga tggaaaaagg ctacacccgt
    14941 aaacaggtgc gtttactgtg cgaatggtat ttgcgcgtac gtaaatcgtc ttaacaaccc
    15001 tggcccggta cacatgttac cgggcctaca acgacagcga accgtgggcc tgagaagcgg
    15061 caacacaggc gtagcataca gttggcaaat gtagtacggg gggcatatga cctggtttat
    15121 tgaccggcgt ctgaacggca aaaacaaaag catggtgaat cgccagcgtt ttttacgccg
    15181 ttataaagcg caaattaaac agtcgatctc cgaggccatt aataagcgtt cggtgactga
    15241 cgtcgacagc ggcgaatccg tatccattcc cacggaagat attagcgaac cgatgtttca
    15301 tcaggggcgt ggcggtctgc gccaccgcgt gcatccgggc aatgaccatt tcgtccagaa
    15361 cgaccgaatt gaacgtcccc agggtggcgg cggaggttcc ggcagtggtc agggccaggc
    15421 cagccaggat ggtgaaggtc aggatgaatt tgtctttcag atttcgaaag atgagtatct
    15481 tgatttgctc tttgaagatt tggccttacc gaatctgaaa caaaaccaac aacgccagct
    15541 gaccgaatat aaaacgcatc gggcgggtta tactgctaac ggcgttccgg ccaatatcag
    15601 cgttgtgcgt tccttgcaga actcactggc gcgacgcaca gccatgacgg caggcaagcg
    15661 gcgggaactt catgcactgg aagagaattt ggccatcatc agcaacagtg aacctgcgca
    15721 gctgctggaa gaggaacgtc tgcgcaaaga aattgcagaa ttacgtgcca aaattgaacg
    15781 cgtccctttt attgacacct tcgatttacg ttacaagaac tacgagaagc ggcccgatcc
    15841 ctccagccag gcagtgatgt tttgcctgat ggacgtttcc ggttcaatgg atcaatccac
    15901 taaagatatg gctaagcgtt tttatattct gctgtatctg ttcctcagca gaacgtataa
    15961 gaacgtggaa gtcgtataca tccgccatca tacccaggcg aaagaagtcg atgaacatga
    16021 gtttttctac tcgcaggaaa caggcggcac cattgtttcc agcgccctga aactgatgga
    16081 tgaggtagtg aaagagcgtt ataacccggc acagtggaat atttacgctg cacaagcatc
    16141 ggacggcgat aactgggccg atgactctcc gctttgccat gaaatcctgg cgaaaaaatt
    16201 attacctgtt gttcgttatt acagctatat cgaaattacc cgtcgtgcac atcagacatt
    16261 gtggcgagaa tatgagcatc tgcaatctac tttcgacaac tttgcgatgc agcacatccg
    16321 cgaccaggat gatatttatc cggtgttccg tgaactgttt cataaacaaa atgcaacagc
    16381 taaagactaa aactatcagc caggtcatta tcgcctggct gatttttagc ttactgtaaa
    16441 ttatctcatt tattacatac attagcttac aatcgcttta
//
LOCUS       DAPHDX010000098        16179 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPHDX010000098 DAPHDX010000000
VERSION     DAPHDX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.099"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..903
                     /gene="repE"
                     /locus_tag="RH058_003190"
     CDS             148..903
                     /gene="repE"
                     /locus_tag="RH058_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDV3138744.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            1491..2657
                     /gene="sopA"
                     /locus_tag="RH058_003191"
     CDS             1491..2657
                     /gene="sopA"
                     /locus_tag="RH058_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDV3138745.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            2657..3628
                     /locus_tag="RH058_003192"
     CDS             2657..3628
                     /locus_tag="RH058_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDV3138746.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            4366..5268
                     /locus_tag="RH058_003193"
     CDS             4366..5268
                     /locus_tag="RH058_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV3138747.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATDEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            5265..5576
                     /locus_tag="RH058_003194"
     CDS             5265..5576
                     /locus_tag="RH058_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138748.1"
                     /translation="MNSDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLAPVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            5652..6335
                     /locus_tag="RH058_003195"
     CDS             5652..6335
                     /locus_tag="RH058_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV3138749.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACIEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPPNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            6336..6557
                     /locus_tag="RH058_003196"
     CDS             6336..6557
                     /locus_tag="RH058_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138750.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            6571..7005
                     /locus_tag="RH058_003197"
     CDS             6571..7005
                     /locus_tag="RH058_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV3138751.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNTREND"
     gene            7050..7820
                     /locus_tag="RH058_003198"
     CDS             7050..7820
                     /locus_tag="RH058_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138752.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVRKARAEIRSMADALAKIRK"
     gene            8360..8662
                     /locus_tag="RH058_003199"
     CDS             8360..8662
                     /locus_tag="RH058_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV3138753.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHLECNAIYAALD"
     gene            8709..9023
                     /locus_tag="RH058_003200"
                     /pseudo
     CDS             8709..9023
                     /locus_tag="RH058_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            9023..12739
                     /gene="traI"
                     /locus_tag="RH058_003201"
                     /pseudo
     CDS             9023..12739
                     /gene="traI"
                     /locus_tag="RH058_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            12759..13505
                     /gene="traX"
                     /locus_tag="RH058_003202"
     CDS             12759..13505
                     /gene="traX"
                     /locus_tag="RH058_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDV3138754.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAEAMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            13564..14424
                     /locus_tag="RH058_003203"
     CDS             13564..14424
                     /locus_tag="RH058_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3138755.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            14527..15084
                     /locus_tag="RH058_003204"
     CDS             14527..15084
                     /locus_tag="RH058_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDV3138756.1"
                     /translation="MTEQKRPVLTLKRKTEGETPIRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            15239..15442
                     /locus_tag="RH058_003205"
     CDS             15239..15442
                     /locus_tag="RH058_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138757.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
BASE COUNT         3744 a         3971 c         4715 g         3749 t
ORIGIN      
        1 gagaaggtgt tgtgacaaat tgcccttaac cctgtgacaa attgccctca gaagaagctg
       61 ttttttcaca aagttatccc tgcttattga ctctttttta tttagtgtga caatctaaaa
      121 acttgtcaca cttcacatgg atctgtcatg gcggaaacag cggttatcaa tcacaagaaa
      181 cgtaaaaata gcccgcgaat cgtccagtca aacgacctca ctgaggcggc atatagtctc
      241 tcccgggatc aaaaacgtat gctgtatctg ttcgttgacc agatcagaaa atctgatggc
      301 accctacagg aacatgacgg tatctgcgag atccatgttg ctaaatatgc tgaaatattc
      361 ggattgacct ctgcggaagc cagtaaggat atacggcagg cattgaagag tttcgcgggg
      421 aaggaagtgg ttttttatcg ccctgaagag gatgccggcg atgaaaaagg ctatgaatct
      481 tttccctggt ttatcaaacg tgcgcacagt ccatccagag ggctttacag tgtacatatc
      541 aacccatatc tcattccctt ctttatcggg ttacagaacc ggtttacgca gtttcggctt
      601 agtgaaacaa aagaaatcac caatccgtat gccatgcgtt tatacgaatc cctgtgtcag
      661 tatcgtaagc cggatggctc aggcatcgtc tctctgaaaa tcgactggat catagagcgt
      721 taccagctgc ctcaaagtta ccagcgtatg cctgacttcc gccgccgctt cctgcaggtc
      781 tgtgttaatg agatcaacag cagaactcca atgcgcctct catacattga gaaaaagaaa
      841 ggccgccaga cgactcatat cgtattttcc ttccgcgata tcacttccat gacgacagga
      901 tagtctgagg gttatctgtc acagatttga gggtggttcg tcacatttat tctgacctac
      961 tgagggtaat ttgtcacagt tttgctgttt ccttcagcct gcatggattt tctcatactt
     1021 tttgaactgt aatttttaag gaagccaaat ttgagggcag tttgtcacag ttgatttcct
     1081 tctctttccc ttcgtcatgt gacctgatat cgggggttag ttcgtcatca ttgatgaggg
     1141 ttgattatca cagtttatta ctctgaattg gctatccgcg tgtgtacctc tacctggagt
     1201 ttttcccacg gtggatattt cttcttgcgc tgagcgtaag agctatctga cagaacagtt
     1261 cttctttgct tcctcgccag ttcgctcgct atgctcggtt acacggctgc ggcgagcgct
     1321 agtgataata agtgactgag gtatgtgctc ttcttatctc cttttgtagt gttgctctta
     1381 ttttaaacaa ctttgcggtt ttttgatgac tttgcgattt tgttgttgct ttgcagtaaa
     1441 ttgcaagatt taataaaaaa acgcaaagca atgattaaag gatgttcaga atgaaactca
     1501 tggaaacact taaccagtgc ataaacgctg gtcatgaaat gacgaaggct atcgccattg
     1561 cacagtttaa tgatgacagc ccggaagcga ggaaaataac ccggcgctgg agaataggtg
     1621 aagcagcgga tttagttggg gtttcttctc aggctatcag agatgccgag aaagcagggc
     1681 gactaccgca cccggatatg gaaattcgag gacgggttga gcaacgtgtt ggttatacaa
     1741 ttgaacaaat taatcatatg cgtgatgtgt ttggtacgcg attgcgacgt gctgaagacg
     1801 tatttccacc ggtgatcggg gttgctgccc ataaaggtgg cgtttacaaa acctcagttt
     1861 ctgttcatct tgctcaggat ctggctctga aggggctacg tgttttgctc gtggaaggta
     1921 acgaccccca gggaacagcc tcaatgtatc acggatgggt accagatctt catattcatg
     1981 cagaagacac tctcctgcct ttctatcttg gggaaaagga cgatgtcact tatgcaataa
     2041 agcccacttg ctggccgggg cttgacatta ttccttcctg tctggctctg caccgtattg
     2101 aaactgagtt aatgggcaaa tttgatgaag gtaaactgcc caccgatcca cacctgatgc
     2161 tccgactggc cattgaaact gttgctcatg actatgatgt catagttatt gacagcgcgc
     2221 ctaacctggg tatcggcacg attaatgtcg tatgtgctgc tgatgtgctg attgttccca
     2281 cgcctgctga gttgtttgac tacacctccg cactgcagtt tttcgatatg cttcgtgatc
     2341 tgctcaagaa cgttgatctt aaagggttcg agcctgatgt acgtattttg cttaccaaat
     2401 acagcaatag taatggctct cagtccccgt ggatggagga gcaaattcgg gatgcctggg
     2461 gaagcatggt tctaaaaaat gttgtacgtg aaacggatga agttggtaaa ggtcagatcc
     2521 ggatgagaac tgtttttgaa caggccattg atcaacgctc ttcaactggt gcctggagaa
     2581 atgctctttc tatttgggaa cctgtctgca atgaaatttt cgatcgtctg attaaaccac
     2641 gctgggagat tagataatga agcgtgcgcc tgttattcca aaacatacgc tcaatactca
     2701 accggttgaa gatacttcgt tatcgacacc agctgccccg atggtggatt cgttaattgc
     2761 gcgcgtagga gtaatggctc gcggtaatgc cattactttg cctgtatgtg gtcgggatgt
     2821 gaagtttact cttgaagtgc tccggggtga tagtgttgag aagacctctc gggtatggtc
     2881 aggtaatgaa cgtgaccagg agctgcttac tgaggacgca ctggatgatc tcatcccttc
     2941 ttttctactg actggtcaac agacaccggc gttcggtcga agagtatctg gtgtcataga
     3001 aattgccgat gggagtcgcc gtcgtaaagc tgctgcactt accgaaagtg attatcgtgt
     3061 tctggttggc gagctggatg atgagcagat ggctgcatta tccagattgg gtaacgatta
     3121 tcgcccaaca agtgcttatg aacgtggtca gcgttatgca agccgattgc agaatgaatt
     3181 tgctggaaat atttctgcgc tggctgatgc ggaaaatatt tcacgtaaga ttattacccg
     3241 ctgtatcaac acagccaaat tgcctaaatc agttgttgct cttttttctc accccggtga
     3301 actatctgcc cggtcaggtg atgcacttca aaaagccttt acagataaag aggaattact
     3361 taagcagcag gcatctaacc ttcatgagca gaaaaaagct ggggtgatat ttgaagctga
     3421 agaagttatc actcttttaa cttctgtgct taaaacgtca tctgcatcaa gaactagttt
     3481 aagctcacga catcagtttg ctcctggagc gacagtattg tataagggcg ataaaatggt
     3541 gcttaacctg gacaggtctc gtgttccaac tgagtgtata gagaaaattg aggccattct
     3601 taaggaactt gaaaagccag caccctgatg cgaccacgtt ttagtctacg tttatctgtc
     3661 tttacttaat gtcctttgtt acaggccaga aagcataact ggcctgaata ttctctctgg
     3721 gcccactgtt ccacttgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     3781 tatcgtcggt ctgattatta gtctgggacc atggtcccac tcgtatcgtc ggtctgatta
     3841 ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacga
     3901 tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact tgtattgtcg
     3961 atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca agtattgaca
     4021 tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc tgctgtggat
     4081 tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac aaaatacctg
     4141 gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc
     4201 gccggcctcc tcacccggtc acgtttcgtc gttcctcctc cacgcgctgc ggcttcgggg
     4261 ccgcacctgc attcgtatgc ggtcgcccgg ttacaggtgc ggcacggcct gatggaggcc
     4321 gcatgtgaga ggagaattcc catgccaaac tggtgctcaa atcgtatgca tttttctggt
     4381 gaaccagcac agatcgctga gattaaacga ctggccagcg gtgcagtcac accgctttat
     4441 cgccgcgcca cagatgaagg tattcagctg tttctggccg gtagtgccgg acttctgcag
     4501 accactgaag atgtgcggtt tgaaccatgc cccggactga cggctgccgg gcgtggtgtt
     4561 gtatcgccgg agaatatcgc gttcacccgc tggctgacac acctgcagaa cggtgtgcta
     4621 ctggatgaac aaaactgcct gatgctgcat gaactctggc tgcagagtgg tactggccag
     4681 cgtcgctggg aaggattacc ggatgaggtc agggaaacca tcaccgtgca tttcaccgcc
     4741 aaaagaggtg actggtgtgg cttctggagt aacgaggatg tatcggtgtg gtggaaccgt
     4801 ctgtgtgaca atgtactgcc ggaaaaaacc atgccgtttg acctgctgac ggttctgccc
     4861 acccgcctgg atgttgaagt gaatggcttt aacggtggtg ttctgaacgg tgttccttct
     4921 gcgtatcact ggtatacgga acagtatggc gtgaagtggc ctgtggggta tgaggtgaat
     4981 atcagcagtc agggagacaa cttcattcag gttgatttcg acacgccgtg gtgtcagccg
     5041 gaaagcgacg ttattgcaga attaagccgc cgtttcagtt gcacactgga gcactggtat
     5101 gccgagcagg gctgcaattt ctgtggctgg cagcgatatg agcgtggaga gcttgttgat
     5161 gtcctgtggg gggaactgga atggtcttcc ccgacagatg acgatgagct gccggaagtc
     5221 accggacctg cgtggattgt cgacaaggtg gcgcattacg gtggatgaat tctgacggag
     5281 acgggcgggc aaccgcccgt ttcttttccg acaaaggatg tcgccgtgct cctcttttta
     5341 ctgcgccggc tgacgcggcg cggcataaac gctgcctgtg gtcagtgtcc cgcgtccggc
     5401 gggcacggga cgggcgcttt taccgctccc ggctggcacc ggtgacagtg tacgccagcc
     5461 cgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg cggcatagcc ttgccgtcaa
     5521 aaagacgacg gctgcggtat tccacggtcg gcctgacccg ttaccggacg cggtgaacag
     5581 cccacaggcc gcggggaacg ggcaccacag gggtgccctc ccggtgcctt ctgtaagaga
     5641 aggaggcttt tatgtcccgt tttatcctgg gtgactgcgt gcgagttatg gccacgtttc
     5701 cggacaatgc tgtcgatttc atcctcaccg acccgccgta tctcgtcggt ttccgtgacc
     5761 gttccgggcg taccattgcc ggcgataaaa ccgatgaatg gctgcaaccg gcctgtattg
     5821 aaatgtttcg cgtactcaaa aaagatgcgc tgatggtcag cttctacggc tggaaccgcg
     5881 tcgatcgctt tatggccgcc tggaaaaatg cgggattcag cgttgttggt cacctggtct
     5941 tcaccaaaaa ctacacatcg aaggccgcat atgtgggcta tcgccacgaa tgcgcctaca
     6001 tcctggcaaa aggccgtcca cgtctgccac caaacccgct gccggacgtg ctgggctgga
     6061 aatattcggg caatcgtcat cacccgacgg aaaagcccgt taccagcctg caaccgctga
     6121 ttgagagctt cacacacccg aacgcaattg tgctggaccc gtttgcaggc agcggctcaa
     6181 cctgcgtcgc cgccctccag tccggacgcc ggtatatcgg tatcgagctg cttgagcagt
     6241 atcaccgtgc cgggcagcaa cgccttgccg ccgtgcaacg ggccatgcag cagggggccg
     6301 cgaatgatga ctggtttatg ccggaggctg cgtaaatgaa ctatgccgga cacgaaaaac
     6361 tgcgcgccga agtggcggag gtggccaatg ccatgtgcga cctgcgtaca accatgaatg
     6421 agatggagca gcggtacagc tttaatgccg acaccctgcc ggaacgtctg gtgcgtcaga
     6481 cgctgtttcg cgcaaaccgc ctcctgatgg aggcatatac cgaaattctg gaactggatg
     6541 cgtgctttaa agattgagaa ggagacgaga atgtacggaa catgcgaaac gctatgccgg
     6601 gagctggcag taaagtatcc gggagacatg ccgctgatgc tggttatctg gtccccggaa
     6661 gagattcagg ccctcgctga cggaatggat atttccctgt ccgatcatga aatcagaacc
     6721 gtcctggcgc gcctggagga catcccggaa gaccagcgga ctgaatccgg tatttcttcc
     6781 ggcgtggcga tggagatcat caataacgtg agcgaaaacc gccaggtgac cgtccctgct
     6841 gaactgctgg cgtccctgat tcagaccgct gaacaggcat tgtggaaacg tgaatgggcc
     6901 gcccgggatc atggcctcgc cgtcccggaa tgcgtcaccc gccgtcaggc ggtgattaat
     6961 caggcccgca ccctgctgaa aaacaacaca cgcgaaaacg actgatgtta tcgccgcctg
     7021 cgggcggcga ttcagggaga ccatgaatca tgaacgaaac gctgaatgca ctgatttgtc
     7081 gccacgcccg taacctgctg ctggcgcagg gctggccgga agagacggat gttgaccagc
     7141 gaaatccgaa ccatccgggc tggatcagca tttacgtgcg gctggatgcg ccccggctgg
     7201 cgacgttact tatcaaccgt cacggcggcg tactgccgcc gcacctggcc tctgccattc
     7261 agaaactgac cggaaccggg gcggaacttg ttctgtccgg cagtcagtgg cagtcgctgc
     7321 cggtacttcc ggcagacgga acgcaggtgt ctttcccgta tgccggtgag tggctggcag
     7381 aagacgaaat cagggctgta cttgcggcgg tgcgcgatgc aatacgaagc atctgttacc
     7441 aggtggcaga cgatgcgcgg cgtatccggg cggcgctgac caccaccggt cagacgttgc
     7501 tgacccgcca gacgcgccgc tttcgtctgg ttgtgaagga aagcgatcac ccctgctggc
     7561 tcgatgaaga tgacgaaaac ctgcccgtgg tgctcgatgc catcgtgaac cggggagcac
     7621 gtttttcgtc ggtggaaatg tacctggtca gcgattgtat tgagcatatc ctgtccagtg
     7681 ggctggcctg cgatgtgctg cgtataccgg atgaaccgcc ccgccgctgg tttgaccgtg
     7741 gtgttctgcg ggaggtggtc cggaaagccc gggccgaaat ccgcagcatg gcggatgccc
     7801 tggcaaaaat ccggaaatga tgacgttggc agggtaaatc cccgcctttt tcctgccggt
     7861 ccccggacag agcacaaagg aagtcgccgc cggctccgct ttacccggcc atgcagggca
     7921 tggccttgtg gtttttcagt tatgtggcct cagcgtcgtg tgcgggctgt gccgtgcctc
     7981 catcttagcc gggctggcgt tgatgcaagg gtacgcttcg ccgctgcggt cacccggtcc
     8041 ctccttcccg tctgccgtga ttttccggtc gttcacccgc tcagatttcg cggtctcacc
     8101 ctgcaactcc cgtcagccgt gtgcgtcagg ctgcggcttc ccttgcgccc ctgcatcccc
     8161 gcctgttcgc ccggctttta tggaggcacg gcaccgcccg gtgtcgcaga atatgtgaac
     8221 aacggaggaa aacatcatgc aatatgcgaa acctgtcact ctgaatgttg aagagtgcga
     8281 ccgtctctcc tttctgccgt atctgtttgg caacgatttt ctgtatgccg aggcgtatgt
     8341 gtacgcgctg gcgcaaaaaa tgatgccgga atatcaggga ggcttctggc acttcatccg
     8401 cctgccggac ggtggcggtt acatgatgcc ggatggcgac cgtttccaca tggtgaacgg
     8461 tgcaaactgg tttgaccgta ccgtgagtgc tgatgccgca ggcatcatcc tgacctccct
     8521 tgtgattaac cgccagttgt ggctgtacca cgacagcggg gatgcaggac tgacccagct
     8581 ttaccggatg cgcgatgcgc agttgtggcg tcacatcgaa tttcacctcg aatgcaacgc
     8641 gatttacgcg gctctggact gattaacgga cggggcggca atgccgcccc tgtaaaggag
     8701 acgagaacat gtactgtact gttaaagaaa ttatccgcga agtgctggat acagacgtgc
     8761 cggacagtga atgcgttttt gccgtagtgc tgacccgtgg ggatgtgcgt cacatagccc
     8821 aggactggag tctgacagac gatgagctgg aaaccgtcat gcagcggctg gacgatgcct
     8881 ttgaatatgg tgcggatgtc agcgttgttc acggcgttgt tcgtgaactg atggaagaaa
     8941 agcgcgccag ccgtcaggtg acagtcccgg cggtgatgct ggaaaaagtg atggcgctgg
     9001 caggcagtga aatgaagcgc ctgtaaccct gctggacggt gccgcacgtc ataacgtaca
     9061 ggtcctgata accgacagcg ggcagcgaac cggtacaggc agtgcactga tggccatgaa
     9121 agatgccggg gtgaacacat atcgctggca ggggggagaa cagcggccgg ccaccatcat
     9181 cagtgaaccg gaccgtaatg tccgctatgc ccggctggca ggagattttg ctgccagcgt
     9241 gaaagccgga gaagagagcg tggcacaggt cagcggggta cgggaacagg ccatactgac
     9301 acaggccatt cgcagtgagc tgaaaacaca gggcgtgctc ggacgcccgg aggtaaccat
     9361 gaccgccctg tcgccggtct ggctggacag ccggagccgt tatctgcggg atatgtaccg
     9421 tccggggatg gtgatggagc agtggaaccc ggagacacgc agtcatgacc gctatgttat
     9481 cgaccgggtg acggcgcaga gtaacagcct gaccctgcgg gatgcgcagg gtgaaacgca
     9541 ggtggtgcgt atttcctccc tggacagcag ctggtcgctg ttccggccgg aaaaaatgcc
     9601 agtggcagac ggcgagcgac tgagggtgac agggaaaatt cccggactcc gcgtctccgg
     9661 cggtgaccgc ctgcaggtgg catccgtcag tgaagatgcg atgacggttg ttgtgccggg
     9721 gcgggctgaa ccggccaccc tgcctgtgag cgattcacca ttcatggcac tgaagctgga
     9781 gaacggctgg gtggaaacgc ctgggcattc cgtcagcgac agtgcgaagg tttttgcctc
     9841 cgtcacacag atggcaatgg acaacgccac cctgaacggt ctggcccgca gcggtcgtga
     9901 tgtccggctg tattcctcac tggatgaaac ccgtactgcg gaaaaacttg cccgccatcc
     9961 ctcctttacg gtggtttctg agcagataaa ggcgcgtgcc ggtgagacat tgctggaaac
    10021 cgctatcagt ctgcagaaaa ccgggcttca cacgccggca cagcaggcta ttcatctggc
    10081 gcttcctgtg gtggaaagta aaaacctggc cttcagcatg gtggacctgc tgacagaggc
    10141 gaagtcgttt gctgcagaag gaaccagttt tactgaactg ggaggggaaa tcaatgcgca
    10201 gataaaacgc ggtgatttac tgtatgtgga tgtggcaaaa ggctatggca caggcctgct
    10261 ggtttcccgt gcgtcgtatg aggcagaaaa gagcattctt cgccatattc tcgaaggtaa
    10321 ggaggcggtc acgccactga tggagagagt acctggcgaa ctcatggaga aactgacatc
    10381 aggacagcgt gccgctaccc gcatgatact ggaaacgtcc gaccgtttca cggtggtgca
    10441 gggctatgcc ggtgtgggta agaccacaca gttccgggcg gtgatgtcag ccgtgaacat
    10501 gctgccggag agtgaacgtc cccgtgtcgt ggggctgggg cccacgcacc gtgcggtcgg
    10561 tgagatgcgc agcgccggcg tggatgcaca gacgctggcg tcctttctgc atgacacgca
    10621 gctgctgcag cgcagcggag aaacgccgaa tttcagcaac acgctgttcc tgctcgatga
    10681 gagctctatg gtgggcaata ccgacatggc acgggcatac gccctgattg cggccggtgg
    10741 cggtcgtgcc gtggccagtg gtgacacgga ccagttgcag gccatcgcgc ccggtcagcc
    10801 tttccgtctc cagcagacgc gcagtgctgc cgatgtggcc atcatgaagg agattgtgcg
    10861 ccagacgccg gagctgcggg aggcggtata cagcctgatt aaccgggatg tggaaagggc
    10921 actgtccggg cttgagagtg tgaaaccgtc tcaggtgcca cgtcaggagg gcgcatgggt
    10981 accggagcac tccgtgacgg agttcagtca cagccaggaa gcgaaactgg cagaagcgca
    11041 gcagaaggcg atgctgaaag gtgaggcttt tccggatatt cccatgacac tgtatgaagc
    11101 cattgtccgc gattataccg gcaggacgcc ggaagcacgg gagcagacgc tgattgtcac
    11161 gcatctgaat gaggaccggc gcgtactgaa cagcatgatt catgatgcac gggaaaaggc
    11221 cggtgagctg ggaaaagagc aggtcatggc gcctgtcctg aacacagcga atatacgcga
    11281 cggggagttg cgtcgtctct ccacctggga gacacatcgg gacgcacttg ccctggtgga
    11341 taatgtgtat caccggattg ccggtatcag caaggatgac gggctgataa ccttgcagga
    11401 tgcggaaggt aacacgcggc tgatttcgcc ccgggaggcg gtggctgaag gcgtcacact
    11461 gtacactccg gacaccatcc gggtgggaac cggtgaccgg atgcgcttca cgaagagtga
    11521 ccgggagcgc ggttatgtgg ccaacagcgt ctggacggtg acagcggttt ccggtgacag
    11581 tgtcacgctg tcggacggac agcagacccg ggtgattcgc cccggtcagg agcgggcaga
    11641 gcaacatatt gacctggcct atgccatcac cgcccacggt gcgcaggggg caagtgaaac
    11701 ctttgccatt gcgcttgaag gcacggaagg taaccggaaa cagatggccg gttttgagtc
    11761 agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag gtgtataccg ataaccgtca
    11821 gggctggacg gatgccatta acaatgccgt acagaaagga actgcccacg atgtgctaga
    11881 gccgaaatcg gaccgggagg tcatgaatgc agagcggctg ttcagtacgg cgcgggagct
    11941 gcgggacgtg gtggccggcc gtgccgttct ccgtcaggca gggctggccg ggggagacag
    12001 tcctgcacgg tttattgcac ccgggcgcaa atatccgcag ccgtatgtgg cactgccggc
    12061 ttttgaccgt aacggcaagt cagctggtat ctggctgaac ccgctgacca cggatgacgg
    12121 aaacgggctg cggggcttca gtggtgaagg ccgggtgaaa ggcagcgggg atgcgcagtt
    12181 cgtggccctg cagggcagcc gtaacggaga gagcctgctg gctgataata tgcaggatgg
    12241 tgtccagatt gcccgtgata atcctgacag cggtgtggtg gtaagaatcg ccggtgaagg
    12301 tcgtccgtgg aatcccggta ccatcaccgg tggtcgcgtg tggggggata tcccggacaa
    12361 cagcgttcag ccgggagccg gaaatggcga accggtcacg gcagaggtac tggcacagcg
    12421 gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgt gcggatgaaa ttgtccgtaa
    12481 aatggcagag aacaaacctg acctgccgga cggcaaaaca gagcaggctg tcagggatat
    12541 tgccgggctg gagcgtgaca ggtctgccat atctgaacgg gaagctgcgc tgccggagag
    12601 tgtactgcgt gaaccacaac gggtgcggga agcggtccgg gaggttgccc gggaaaatct
    12661 gctgcaggag cgactgcagc agatggagcg ggatatggtt cgtgacctgc agaaagagaa
    12721 aaccctgggc ggagactgac acaggaagat aaacgctgat gacaacggat aacacgaaca
    12781 caacacgtaa tgattcactg gctgcccgga ccgatacctg gttgcagtcg ttgctggtct
    12841 ggtcacccgg acagcgggat atcatcaaaa cggtggcact ggtgctgatg gttctggacc
    12901 atgcaaaccg cattctgcat ctggaccagt cgtggatgtt tctggtcggg cgtggggcct
    12961 ttccgctgtt tgccctggtg tgggggctga atctgtcccg tcatacgcat atccggcagg
    13021 aggccattaa ccggctgtgg ggatgggcgg tgattgccca gttcgcatat tatctggccg
    13081 gctttccctg gtatgagggg aatatcctgt ttgcctttgc agttgcggca caggtgctga
    13141 catggtgtga aacgcgcacc tggtggcgtt ccgcagaggc catgttgctt ctggcaatgt
    13201 ggttgccttt ctccggcacc agttacggca ttgccgggct gctgatgctg gcagtcagcc
    13261 acaggctgta ccgggcggaa gacagaatgg aacgtctggc gctggtcgcc tgcctgctgg
    13321 ctgttattcc ggcgcttaac cttgccacca gtgatgcagc ggcggtggcc ggccttgtga
    13381 tgacggtgct gactgttggt ctggtgtcct gtacagggaa atcactaccc cgtttctggt
    13441 acggggattt tttcccgacg ttctatgcct gtcatcttac tgtgctgggc gttctggctg
    13501 tgtgactggt atgatgctgg cgctgattct gcttattaat aaacaacata acacaggata
    13561 tttatgaaaa ttacggatca caaactccct gaaggtattg ccctgacatt tcgtgtcccg
    13621 gaaggaaata taaaacatcc tttgattctc ttatgccatg gattttgcgg tatccggaat
    13681 gttctgctgc cttcctttgc gaatgcgttt acagaagccg ggtttgcaac gattactttt
    13741 gattatcgtg gattcggtga gagtgaaggg gaacgaggtc gtctggttcc ggcaatgcag
    13801 accgaagata ttatttcagt tatcaactgg gcagaaaagc aggtatgtat tgataatcaa
    13861 cgtataggat tatggggaac atcgctgggt gggtgtcatg tattcaatgc agctgcgcag
    13921 gataaaagag tgaaaagtat tgtcagccag ctggcttttg ctgacggtga ggttctggtt
    13981 accggagaaa tgaatgaact ggagaaagct tcttttctgt ccacgctgaa taaaatggca
    14041 gaaaagaaga aaaataccgg gaaagagatg tttgtggggg ttacccgggt actgagtgat
    14101 aatgaatcaa aggtattctt cgagaaggta aaaggtcagt atcctgaaat ggatataaaa
    14161 atacctttcc ttacggttat ggagacgttg caatataaac cggcagagag cgcagccaaa
    14221 gtacagtgcc cagttttggt tgttattgcc ggccaggata gtgttaatcc accagaacag
    14281 ggaagagcct tatatgatgc tgttgcttct ggtactaagg aattatacga ggaagctgac
    14341 gcatgccatt atgatattta tgaaggcgcg ttttttgaac gtgtggcggc agtacagacg
    14401 caatggttta aaaaacatct ttaattgtcg gtattaatat gtcagaggtc gttgaggtca
    14461 gcttaatatc atagtgagta gtttcaacga cctggaggac atattttcac aaaggtcagg
    14521 gcaatcatga cagagcagaa acgaccggta ctgacactga aacggaaaac ggaaggggaa
    14581 acaccgatcc ggagccggaa aaccatcatc aatgtcacca cgccaccaaa atggaaggtg
    14641 aaaaagcaga aactggcgga gaaggctgcc cgggaagcag agctggcggc gaagaaagca
    14701 caggccagac aggcgctgtc catttatctg acgctgccct cgctggatga ggcggtgaac
    14761 actctgaaac cctggtggcc gggattgttt gacggtaaca cgccccggct tcttgcctgt
    14821 ggtatccggg aagttttact ggatgaggtg tctcagcgga atatcccgct ctctcataaa
    14881 aaactgcgca gggcactgaa ggccatcacc cgttcagaaa gctatcttgg cgccatgaaa
    14941 gccggtgcct gccgctatga tacagaaggc tatgtgacag aacatatcac acaggaagaa
    15001 gaacagtatg cgcaggcgcg cctggaaaag gtgcgcagac agaaccggat caaggatgag
    15061 ctgcgggcga tcctggcaga ataataaaag cgccctctgt atgacagagg gcaacagcga
    15121 aaaaaatcgg gaaaacagac atccagagtt ttgatggaga aaaacatcca accgttcagg
    15181 gatgtttcat aatgtttctg acataatccg ggtgtcgggt caagaggagg gataaaaaat
    15241 ggctgtatcc agtggattat ctgaacacag cagtatttat cgctatcagc gcgaggggcg
    15301 tcatgccgtc cgtcaggaga ttcgtggctt tttcagggaa gcggcagagg tctggcagaa
    15361 tgcggcccgg ctggcaccct gtgaagcatg gcgggatttt gctctgaaga gggcatctgc
    15421 atgtcggtgt aagtgccgat aaaaataacc cccgtcgcgg agctccgggg gatcagactt
    15481 atatggcaga tcgttattat tattatagtg gtgtaagaag gagttgccct gtctttcaaa
    15541 cggacatcac agaattaaca tatttcacat cccgggtaag ccagcaggga taaaaaatgt
    15601 gatgaatttc aagaagcagg aggtgacagt ggcatgcata acagtagggt tatccggtga
    15661 atctgacaga cagccgggct gtattcgcgg caggataaag aaaaaccggg agatgactct
    15721 cccggtccgt cttatcgcta atcccctgag cagggcatgt atgacttatc tatgaccgtt
    15781 atattcagcg ccacatccgg tagcctgcga tataacacag ttatcctgtc agcaagagca
    15841 ggtatgatga atacacggag attaaaaata taatctccgt cacatgttac ccacggaaac
    15901 cggtaacgcc ttcacgggaa atatgtgaca gaaaaagcag cctccgttac tccaggtatc
    15961 aggaggctga ataatgcatg agacgttgtt tcttatgtgt ctggtcaggc tggctgacca
    16021 gcgtttttag taacacatag ccatatgtgt attaagtcac ctcatcttat tttgttgaat
    16081 tgatcacaac tcaacggttt attttttgcg tgatacgtga gccattattc atcccggcat
    16141 ggaattcatg caatcacgaa aagtaatcag tgacggaga
//
LOCUS       DAPHDX010000099        15865 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPHDX010000099 DAPHDX010000000
VERSION     DAPHDX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.100"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..131
                     /locus_tag="RH058_003206"
     CDS             <1..131
                     /locus_tag="RH058_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138758.1"
                     /translation="ADITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            118..342
                     /locus_tag="RH058_003207"
     CDS             118..342
                     /locus_tag="RH058_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV3138759.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(409..1131)
                     /gene="sdiA"
                     /locus_tag="RH058_003208"
     CDS             complement(409..1131)
                     /gene="sdiA"
                     /locus_tag="RH058_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV3138760.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1361..2113)
                     /gene="yecC"
                     /locus_tag="RH058_003209"
     CDS             complement(1361..2113)
                     /gene="yecC"
                     /locus_tag="RH058_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV3138761.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(2110..2778)
                     /gene="tcyL"
                     /locus_tag="RH058_003210"
     CDS             complement(2110..2778)
                     /gene="tcyL"
                     /locus_tag="RH058_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV3138762.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2793..3779)
                     /gene="dcyD"
                     /locus_tag="RH058_003211"
     CDS             complement(2793..3779)
                     /gene="dcyD"
                     /locus_tag="RH058_003211"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV3138763.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELTTRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3884..4684)
                     /gene="tcyJ"
                     /locus_tag="RH058_003212"
     CDS             complement(3884..4684)
                     /gene="tcyJ"
                     /locus_tag="RH058_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3138764.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4772..5323)
                     /gene="fliZ"
                     /locus_tag="RH058_003213"
     CDS             complement(4772..5323)
                     /gene="fliZ"
                     /locus_tag="RH058_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV3138765.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5369..6088)
                     /gene="fliA"
                     /locus_tag="RH058_003214"
     CDS             complement(5369..6088)
                     /gene="fliA"
                     /locus_tag="RH058_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV3138766.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLILYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6253..7728)
                     /locus_tag="RH058_003215"
     CDS             complement(6253..7728)
                     /locus_tag="RH058_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDV3138767.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYQINGTDNYTVNVDSGVVQDK
                     DGKQVYVSAADGSLTTSSDTQFKIDATKLAVAAKDLAQGNKIVYEGIEFTNTGTGAIT
                     ATGNGKLTANVDGKAVEFTISGSTDTSGTSATVAPTTALYKNSAGQLTATKVENKAAT
                     LSDLDLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNE
                     DAAKSLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRI
                     EDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            7983..9395
                     /gene="fliD"
                     /locus_tag="RH058_003216"
     CDS             7983..9395
                     /gene="fliD"
                     /locus_tag="RH058_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV3138768.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPIIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            9410..9820
                     /gene="fliS"
                     /locus_tag="RH058_003217"
     CDS             9410..9820
                     /gene="fliS"
                     /locus_tag="RH058_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV3138769.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9820..10185
                     /gene="fliT"
                     /locus_tag="RH058_003218"
     CDS             9820..10185
                     /gene="fliT"
                     /locus_tag="RH058_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV3138770.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWEELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10263..11750
                     /gene="amyA"
                     /locus_tag="RH058_003219"
     CDS             10263..11750
                     /gene="amyA"
                     /locus_tag="RH058_003219"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV3138771.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVESWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11784..12197)
                     /locus_tag="RH058_003220"
     CDS             complement(11784..12197)
                     /locus_tag="RH058_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3138772.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12384..13589
                     /gene="yedE"
                     /locus_tag="RH058_003221"
     CDS             12384..13589
                     /gene="yedE"
                     /locus_tag="RH058_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV3138773.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPSSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            13586..13819
                     /gene="yedF"
                     /locus_tag="RH058_003222"
     CDS             13586..13819
                     /gene="yedF"
                     /locus_tag="RH058_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV3138774.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVMSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13928..14599
                     /locus_tag="RH058_003223"
     CDS             13928..14599
                     /locus_tag="RH058_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV3138775.1"
                     /translation="MCGRFDQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(14639..15847)
                     /locus_tag="RH058_003224"
     CDS             complement(14639..15847)
                     /locus_tag="RH058_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3138776.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
BASE COUNT         3953 a         3985 c         3850 g         4077 t
ORIGIN      
        1 ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact
       61 ccgccgcctt aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg
      121 agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc
      181 ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg
      241 atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt
      301 tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag
      361 cgtgcctttc agccggtttt tgcatctggc acgcagaaag aaaagcgatc aaattaagcc
      421 agtagcggcc gcgtaacagg ccacctgggt cttatttggt gcattgattt ttttctgcat
      481 gtttttctga tggaaattga ccgtattctc agagattgac aaaatcatcg ctatctctgc
      541 tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat
      601 ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac
      661 cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca
      721 acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc
      781 gcggcgtaaa ccatgtgcgc gcgcggcttc ccataacggc tgtgcttcgc tgaataagtc
      841 atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcaccg gatcaattgc
      901 gagaaagttt tttgcctgat aataactaac ccacgcctca gggtaattgg tgtagaaagc
      961 cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc
     1021 cagctgctga gcctgaagct ctatttcatg gtagacctct tctgcggcct ccatcctctg
     1081 aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg
     1141 caacgcccct gagagtgagt atcatttata atgataattc aaactatcac ataaataaga
     1201 tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct
     1261 tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc
     1321 ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagc agaaacttct
     1381 cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa taacgctttt gctgccccct
     1441 gctcgactat ccgcccctgg tccataaaaa tcgcccggtc cgcaacatcc cgggcaaagc
     1501 tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggattgtgt
     1561 tcaggacttc gcccaccagc tctggatcca gcgctgacgt tggctcgtca aacaaaatca
     1621 cctcaggacg cattgccagc gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca
     1681 aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag
     1741 cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt
     1801 tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct
     1861 gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga
     1921 tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctgcgtagca
     1981 acgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct
     2041 cgaggtcgat accgtgcagc accgtctgac cgtgaaattt tttcaccagg ttcttaactt
     2101 caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg
     2161 caacgtcgat aatactgtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt
     2221 gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcttga cggaacagct ccggcacctg
     2281 gattgtcgcg gccagcgacg tatcttttac caggctgata aagctgttcg acagcggcgg
     2341 caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat
     2401 accaatactg gcagcggctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag
     2461 cgtttcggca gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg
     2521 atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag
     2581 cggtgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg
     2641 cgacagacgc atcagcgcga gaataaaccc tagcagtaag ccaaaaaaca tgccgccgat
     2701 actgagttgc agggtatacc cagccccttt caacaggaac ggcaaggaat caataaccag
     2761 ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc
     2821 gcaggcgcgc cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga
     2881 ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg
     2941 ccttccagac gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag
     3001 ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct
     3061 ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac
     3121 acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccaatccg
     3181 gcgtgagttc cggcactgcc cgatgcgacc accaccgacg aaatattaac cgccccttca
     3241 cactgttgcg cgatttccag cgcactctcc acataaccca gcgcgcccag agcattagaa
     3301 ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgtcagctct
     3361 tccagttggg cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga
     3421 tccagcaaca aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt
     3481 tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca
     3541 tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg cagggcatct
     3601 gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg tgtgacgtca
     3661 tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc
     3721 ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata
     3781 acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata
     3841 aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag
     3901 caccaaacca tttttcggaa agagcttgca gagtgccgtc tttttgcatt tccgcaattg
     3961 catcattcac tgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct
     4021 gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttt accagatcca
     4081 gcgctgccag acggtcaacg aggatcgcat cgatacggcc tacgcgcaga tcctgatatt
     4141 tggtcgggtc atcatcatag gtacgcacat cgacgccctg aacattctgc cgcagccact
     4201 cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt
     4261 taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtaagggg
     4321 tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctgatta atcaccacat
     4381 caatgcgttt agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg
     4441 cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt
     4501 tgccgtcatc cccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg
     4561 tgccgcgctc tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc
     4621 ccgcaaccag cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt
     4681 tcatattcac cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca
     4741 caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg
     4801 aggatttctg cacaagtccg gtacaggttt gccgctgata gtgttgatac ttccgtaatg
     4861 cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga
     4921 aaccgtcctg cagctgatcg agggaaatat tttgctcgtg tagatgattc cccaaacggc
     4981 gcagacgaac gacatattca cgcaccgtac cagggctcat ttcagtttgc tcaaaaagaa
     5041 attgcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc
     5101 aaaatcggca caatgccgcc catgattttt gttccgtttg ccagccattt tcatcaagca
     5161 gcgcgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga acgagggtaa
     5221 tgcgatcgag taatttacgg caatgcgcgc aatgagtctg gctgtgttta aagtctttaa
     5281 gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt
     5341 caaaattaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt
     5401 taatggcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa
     5461 tctctttgag attcagctct tcctggtaat agaggattaa taccagtttt tcgcgctccg
     5521 gcaacgtttc gatggcttcc atcacccgct ggcgcagatt attgtccagc agttgttgta
     5581 gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc
     5641 gccactcatc gtaggagaag agctggctgt tattggtgtc gagcaacatt tggcgataat
     5701 cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt
     5761 cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg
     5821 gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt
     5881 aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacggcatta agtaacccaa
     5941 tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc
     6001 gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta
     6061 caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg
     6121 catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt
     6181 atttgggggt taccggtaaa ttgcgggcag aaaaaacccc gccggtggcg gggtttgagc
     6241 gataagtgta aattaacgca gcagagacag tacgttctgc gtggtctggt tagcctgtgc
     6301 cagaacagag gtacccgctt gttgcaggat ctgcgcacga gacatgttag acacttcggt
     6361 cgcgtagtca gcatcttcga tacggctacg ggcagaagac aggttgttta cggtgttgcc
     6421 aaggttggtg atggcagagt cgaaacggtt ttgtactgca ccgaggtcag aacgcagatt
     6481 gtcaacttta gccaatgctt tgtcgatagt ttcgagcggg ttggtggtag attgcaacga
     6541 ttttgctgca tcttcgttta ccagaatcgg gctaccacct tctgatttgc tcagatacat
     6601 gactttattg ttaccagaag cagtctccgt tatcgtttta ccatctgcac taacatcgta
     6661 agttgcaccg ttaacaacta acgtgcttcc tgttttcttg gcagcgttca gatcaagatc
     6721 agatagtgtc gctgctttat tttcaacttt tgttgcagtc aattgccctg cactattttt
     6781 gtatagggct gtcgtagggg caacggttgc actagtacct gatgtatcag tactccccga
     6841 aatagtgaat tcaacagcct taccatcaac attggcggtt aatttaccat tacctgtggc
     6901 agttatagcg ccagtgccgg tatttgtaaa ttcgatacct tcgtagacaa tcttattacc
     6961 ttgagctaaa tctttagcag ccactgcaag cttagttgca tcaatcttga attgagtatc
     7021 actgctggtc gtaagtgaac catccgcagc actcacataa acttgtttgc catctttatc
     7081 ctgtactact ccactatcta cattaacagt atagttatca gtaccgttaa tttgataatt
     7141 atcagtacct gtcgctttaa atttagaaat caggtcactg ccggttgctt tctgcgcgcc
     7201 atcgatatta aaaccgtcca ggccgagagt tttcgcatca atttttgcca gattgatagt
     7261 gatggtttca ccatcattag caccaacctg aattttcatt tcattatttt cagcaaggac
     7321 tttcacgccg ttaaactgag tttgctcaga tacacggtca atttcttcca gacgttgagt
     7381 aatttcagcc tggatagaag aaagatcgct gtcagagtta gtaccgttag ttgcctgaac
     7441 agaaagttca cgaatacgct gcaggttgtt gttaatttca ttcagcgcac cttcagtggt
     7501 ctgcgcaaca gaaataccat cattcgcgtt acgggaagcc tgggtcagac ctttaatatt
     7561 tgccgtaaaa cggttagcaa tcgcctgacc tgctgcatcg tcttttgcgc tgttaatacg
     7621 cagaccagaa gacagacgct caatagcaga gctaagagaa gactgagatt tgttcaggtt
     7681 attctgggtc aacagcgaca ggctgtttgt attaatgact tgtgccatga tcttttcctt
     7741 atcaattaca acttgatgtt attgggctgt tgcccacggt ttctcaccgt atcccttgta
     7801 tcggcacctg aatttcgaac tttagaaaat tttagtttct ctcctgtttt ttcttaacgg
     7861 ctgaaatatc cgtaatatac aactctactc tgcgcatcat cttcgtgaat caactattaa
     7921 actttgcgca caaattgccg ataacccgct tatctactgt ttgcaatcaa aaggaattag
     7981 gtatggcttc atttacatct cttggcgttg gttcgaatct gccgctggat acattgctaa
     8041 acaacctgac catcgctgaa aaaaaacgct taaacccaat tactcagcaa cagagtgaca
     8101 ataccgcacg tctgacagcc tatggcacat taaaaagtgc attagaaaaa ttccagactg
     8161 caaatactgc gttaaataaa gccgatctgt tcaaaagcac taccgttacc agtagcaatg
     8221 aagaccttaa agtttccacc gaagcaggtg ctgcccccgg tatctatacc atcagcgtaa
     8281 cccaactggc gcaggcgcaa tctttacgaa ccgattcccc gataatcatt gcatcaacaa
     8341 aagatgcact gggtgatgag tcatcagata cacgcacaat aaaaattacc caagacggcc
     8401 gtaaagagcc gctggaaatc aagctcaata aagatcagac gtcattggat gagatcagca
     8461 aagccattaa cgatgccgat agtggaattt ccgctagtat cgttaaagtc aaagatggtg
     8521 actatcaatt ggtattgacc gccagtgaag gtctggccaa taaaatgacc atatctgttg
     8581 agggtgacag taaattaaat gatctgctgg cttatgacag caagaccaac accgggaata
     8641 tgaaggaact ggttaacgcg caaaatgcac agctaaacgt taacggtatc gatattgaac
     8701 gttcaagtaa caaaattacc gatgctcctc agggagtgac tctcgatttg accaaaaaag
     8761 tcactgacgt ccgggtcacg gtgacaaaaa gcaacgataa agcaaccgaa gcaattaaag
     8821 gctgggtgga ttcatataac tccctcattg acacctttaa caccctgaca aaatataaag
     8881 aagtagaccc aggtgctgaa gcacaagata aaaataatgg tgcacttctc ggagacagcg
     8941 ttgtacgtac aattcaaagc ggcatccgcg cacaatttgc aaacggtgcc agcgacggtg
     9001 ccttcaaaac attaaacgag atcgggatta agcaggacgg cacgacgggt aagctgaaaa
     9061 ttgatgatga taaactcaaa aaagtcctga atgaaaatac agcatccgta cgtgaattgc
     9121 tggtaggcga tggtaaagaa acaggaatta ccaccaaaat tgccaccgag gtaaaaggtt
     9181 accttgccga tgatggcatt attgatagcg cacaggacag tattaacgcc acactgaaaa
     9241 aactgaccaa acagtatcta tccgtcagtg ccagcattga tgacaccgtg gcgcgttaca
     9301 cggcacagtt tacgcaatta gataccatga tgagcaaact gaataacacc agtacctacc
     9361 tcagccaaca atttactgca atgagtaatt cctgatcacg gaggtcagca tgtataccgc
     9421 aagcggctca aaagcctatg cacaaattgg cgtcgaaagc gccgtaatga gcgcaagcca
     9481 gcagcagctg gtcaccatgc tatttgatgg agtgctgagc gcactggtta gagcgcgcct
     9541 gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag cgatcaacat
     9601 cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat taacccaaaa
     9661 cttgattgct ctttatagct atatggtcag gcgcttgctg caagccaatt tacgcaacga
     9721 tgtctccgca gtcgaagaag tggaagcatt aatgcgtaat attgccgatg cctggaaaga
     9781 gtcgttactc tccccttctt tgattcagga cccagtctga tgaaccatgc accgcatttg
     9841 tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct ggcaacggaa
     9901 gaacaatggg aagaactcat cgccagcgaa atggcgtatg tgaatgcggt gcaggagatt
     9961 gcacatctga ctgaagagat tgacccgtcc accacgatgc aggagcagct ccgcccgatg
    10021 ctgcgcctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat tcggatggat
    10081 gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag tgcctatggc
    10141 gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctg aacgagtcga
    10201 tggctcgcga ataatccgat tacggctacg cttctaatgt tccccttgaa tggagtcgaa
    10261 gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa ggcggtaagc
    10321 tctggcctga actggccgag cgcgccgacg gttttaatga tattggtatc aatatggtct
    10381 ggttgccgcc cgcctataaa ggcgcatcgg gcgggtattc ggtcggctac gactcctatg
    10441 atttatttga tttaggcgag tttgatcaga aaggcagcat ccctactaaa tatggcgata
    10501 aagcacaact gctggccgcc attgacgctc tgaaacgtaa tgacattgcg gtgctgttgg
    10561 acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc gtgcagcgtg
    10621 taaatgctga tgaccgtacg caaattgacg aagaaatcat agagtgcgaa ggctggacgc
    10681 gttacacctt ccccgcccgt gccgggcaat actcgcagtt tatctgggat ttcaaatgtt
    10741 ttagcggtat cgaccatatc gaaaaccctg atgaagatgg catttttaaa attgttaacg
    10801 actacaccgg tgaaggctgg aacgatcagg ttgatgatga actcggtaat ttcgattatc
    10861 tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt aaatactggg
    10921 cgcgctgggt gatggaacaa acacaatgcg acggttttcg tcttgatgcg gtcaaacata
    10981 ttccggcgtg gttttataaa gagtggatcg aacacgtaca ggaagttgcg ccaaagccgc
    11041 tgtttattgt ggcagagtac tggtcgcatg aagtcgataa actgcaaacg tatattgatc
    11101 aggtggaagg caaaaccatg ctgttcgatg cgccgctgca gatgaaattc catgaagcat
    11161 cgcgcatggg gcgcgactac gacatgacgc agattttcac gggtacatta gtggaagccg
    11221 atcctttcca cgccgtgacg ctcgttgcca atcacgacac ccaaccattg caagccctcg
    11281 aagcgccggt cgaatcgtgg tttaaaccgc tggcgtacgc cctaattttg ttgcgggaaa
    11341 atggcgttcc ttcggtgttc tatccggatc tctacggtgc acattacgaa gatgtcggtg
    11401 gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat gagttaattc
    11461 tcgcccgtca gcgtttcgcc cacggtgtac agacgttatt tttcgaccat ccgaactgca
    11521 ttgcctttag ccgcagtggc actgacgaat atcccggctg cgtggtggtc atgtcgaacg
    11581 gggatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa acctggcgtg
    11641 attttctggg gaaccggcaa gagagcgtag tgaccgacga aaacggcgaa gcaaccttct
    11701 tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa attcatcccc
    11761 ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc tttatccagc
    11821 gcggcggcac actctgccgt cgggcgatcc actcgctcca tcgtcatacc atcgtactca
    11881 atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac gttagtgaga
    11941 tcatcagaca tcaatgtcaa cttgcccggc acggcaatca cccgctgcca ctgacggcaa
    12001 tccagggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg actgaccagc
    12061 gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc gggggttttc
    12121 acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag cgcgcttgca
    12181 attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt ggcattaaag
    12241 ccctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta ttatttttgt
    12301 gaattattac cccgaaaata aatttgcatg atgtatgatt cgcgattctt gtttcaaacc
    12361 ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc tggttgatta
    12421 aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact tactattttg
    12481 gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc ggtcagctcc
    12541 tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc catctggaag
    12601 gatcgccatt aacccgcatc gacgggatga tgatcctcgg catgtttggc ggctgctttg
    12661 ccgcagcgct gtgggccaac aatgtcaaac tgcgcatgcc gagcagccgt atccgcatta
    12721 tgcaggccat tattggcggc attatcgccg gttttggcgc gcgtctggca atgggctgta
    12781 acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg ttctttgcca
    12841 tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc atcttccgta
    12901 ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa ccggatcagg
    12961 cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc tgggcgctgc
    13021 tcacagcgat gaaccaaccc aaactggggc tggcaatgct gtttggcgtc ggctttggtt
    13081 tactgattga acgtgcgcaa atctgcttta cttcagcgtt ccgcgatatg tggatcaccg
    13141 gacgtaccca tatggcgaaa gcaatcatta ttggtatggc ggtgagtgcc atcgggatct
    13201 tcagttacgt acagttaggc gttgaaccca aaatcatgtg ggcgggacca aacgcggtaa
    13261 ttggtggttt actgtttggt tttggcatcg tgctggcagg cggctgcgaa accggctgga
    13321 tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc aacgtgatcg
    13381 gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc accgactggg
    13441 acaaaatcaa cctgctgaaa acctttggcc cgatgggtgg cctgctggtg acatatttgc
    13501 tgttgtttac tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc cgccgtgcgg
    13561 caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc gccttgatat
    13621 ggtgggtgaa ccctgccctt atccggcagt cgcaactctt gaggcgatgc cgcagttaaa
    13681 aaaaggggaa atcctggaag tgatgagcga ctgtccacag tcgatcaata atattccact
    13741 ggatgcgcgt aatcacggct atacggtgct ggatattcag caagacgggc cgaccattcg
    13801 ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga acatcgttac
    13861 ctttgtatca catagatttt acccgccccc tctcctattc ttttcagtat gatctcagga
    13921 ggtttctatg tgtggacgct ttgaccaatc ccaaacgcgt gtagattacc ttgcgcttct
    13981 cgcggaagat attgaacgcg atattcccta tgatcccgaa cccattggca gatacaacgt
    14041 cgcgccggga accaaagtcc ttctgctcag tgaacgtgac gaacaccttc atctggatcc
    14101 ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccactgatta acgcccgcgt
    14161 agaaactgcc gcaaccagtc gcatgtttaa accgctctgg caacatggtc gggcaatctg
    14221 ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaacagc cttattttat
    14281 ctatcgcgct gatggacaac ctgtttttat ggccgcgata ggtagcacac cgtttgagcg
    14341 cggtgacgaa gccgaaggat ttttaattgt cactgcagca gcagaccagg gtctggtaga
    14401 tattcatgac cgccgcccgc tggttctgtc gccagaagct gcacaggaat ggatgcggca
    14461 agagattggc ggtaaagaag catcagaaat tgctaccaat ggctgtgtgc cagctaacca
    14521 gttcacctgg cacccggtat cgcgcgcggt aggtaatgtc aaaaaccagg gcgcggagtt
    14581 aattcaacct gtttgttgac atcctccacg ccctgaagga cgaggatccc tgctacgttc
    14641 aggctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa cgggcggcct gactgcacca
    14701 tctctccaca ggcaagaacg gcgtgccccg cagctaaaat gttacgagcg ccatttacat
    14761 cggcgttcgc tgtatatcca catacctggc atctgaattt actttgtgac aggcggtttt
    14821 ctttcgctgt atgaccacag cacgcgcaac gctggcttgt gtacgctggc ggaacagcaa
    14881 gcacctgacc gccacgccag agctgcttgt actcaagctg gcggcgcatt tcataccagc
    14941 cctgatccag tatcgaacgg tttaaacctg attttgcccg gacattgcgc cccggctggc
    15001 tgaccgtacc cgctgctgac tttgacatgt tgctgacctt caaatcctca atgactatca
    15061 ttgcgtggtt tttgctgacg gtcgttgtga ctttgtgaag gtagtccctg cggatatttg
    15121 cgatacagga atgcagtcgc tgtattttgc gtttctgctt ctgccagttg ttgctgaatt
    15181 tgaccttgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt ttctgaaaac
    15241 tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc acgccagcat
    15301 ccagcccgac cattgatgct gaagggtgaa ccggagttga tacttcactt tctgtctgaa
    15361 tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca acacccgtga
    15421 cctgacggct gttccggtag cgcatccagc ccagtttcgg cagaaaaata cggctgtttt
    15481 cctgatcgag cttaacgccc tgcgggtagc ggaatacatc attctgtccc cgctttttga
    15541 atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca aggtctttca
    15601 gtgactgttg caatggctgt gagggagaat ctttaagcca ttgcgtttca gtggcatttt
    15661 tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat
    15721 gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc
    15781 gcctcatttc acactcctgt tgaccacctg gtcttaactg gaatttaaat gcctgtaatc
    15841 gtttcacagg ccaaatgata ctttg
//
LOCUS       DAPHDX010000100        15467 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPHDX010000100 DAPHDX010000000
VERSION     DAPHDX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.101"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            473..2104
                     /gene="oppA"
                     /locus_tag="RH058_003225"
     CDS             473..2104
                     /gene="oppA"
                     /locus_tag="RH058_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV3138777.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2190..3110
                     /gene="oppB"
                     /locus_tag="RH058_003226"
     CDS             2190..3110
                     /gene="oppB"
                     /locus_tag="RH058_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV3138778.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3125..4033
                     /gene="oppC"
                     /locus_tag="RH058_003227"
     CDS             3125..4033
                     /gene="oppC"
                     /locus_tag="RH058_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV3138779.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4045..5058
                     /gene="oppD"
                     /locus_tag="RH058_003228"
     CDS             4045..5058
                     /gene="oppD"
                     /locus_tag="RH058_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV3138780.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5055..6059
                     /gene="oppF"
                     /locus_tag="RH058_003229"
     CDS             5055..6059
                     /gene="oppF"
                     /locus_tag="RH058_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV3138781.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6112..6441)
                     /locus_tag="RH058_003230"
     CDS             complement(6112..6441)
                     /locus_tag="RH058_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV3138782.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6476..7936)
                     /gene="cls"
                     /locus_tag="RH058_003231"
     CDS             complement(6476..7936)
                     /gene="cls"
                     /locus_tag="RH058_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV3138783.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8079..8252
                     /locus_tag="RH058_003232"
     CDS             8079..8252
                     /locus_tag="RH058_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV3138784.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8307..9560)
                     /gene="kch"
                     /locus_tag="RH058_003233"
     CDS             complement(8307..9560)
                     /gene="kch"
                     /locus_tag="RH058_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV3138785.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9859..10155)
                     /locus_tag="RH058_003234"
     CDS             complement(9859..10155)
                     /locus_tag="RH058_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV3138786.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10379..11098
                     /gene="tonB"
                     /locus_tag="RH058_003235"
     CDS             10379..11098
                     /gene="tonB"
                     /locus_tag="RH058_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV3138787.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQLPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11138..11536)
                     /gene="yciA"
                     /locus_tag="RH058_003236"
     CDS             complement(11138..11536)
                     /gene="yciA"
                     /locus_tag="RH058_003236"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV3138788.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11641..12180)
                     /locus_tag="RH058_003237"
     CDS             complement(11641..12180)
                     /locus_tag="RH058_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV3138789.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12210..12953)
                     /locus_tag="RH058_003238"
     CDS             complement(12210..12953)
                     /locus_tag="RH058_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV3138790.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12981..13070)
                     /locus_tag="RH058_003239"
                     /pseudo
     CDS             complement(12981..13070)
                     /locus_tag="RH058_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13310..13948
                     /gene="ompW"
                     /locus_tag="RH058_003240"
     CDS             13310..13948
                     /gene="ompW"
                     /locus_tag="RH058_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV3138791.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(14008..14514)
                     /locus_tag="RH058_003241"
     CDS             complement(14008..14514)
                     /locus_tag="RH058_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV3138792.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14560..15060)
                     /locus_tag="RH058_003242"
     CDS             complement(14560..15060)
                     /locus_tag="RH058_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV3138793.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(15146..15325)
                     /locus_tag="RH058_003243"
     CDS             complement(15146..15325)
                     /locus_tag="RH058_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV3138794.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4119 a         3607 c         3733 g         4008 t
ORIGIN      
        1 tgttaaatta ttgtgcattt cactactgga actgtaatca gaaaagatag acatgcttag
       61 ccaatctcta tttgattgaa ttgaaagatg tttgttaagg catggatgca agctatagat
      121 tctgatacgg tcaataaaag agaattgctt aacaattttg caaaatgtat tggcgagtaa
      181 gaaccgcatt tggtactttc cgggcaaccg ccagacgatt atttattggt aatgagaata
      241 attaacaatt aaagagcgtc gcgaaagaat aatgtgtctc gacaggggag acacagtacg
      301 aatcgacata aggtgatcgt ctgaatcacc agaataaata aagtcggtga tagtaatacg
      361 taacgataaa gtaacctgac agcagaaagt ctccgagcct gtgcagggtc ccaatccggg
      421 attacacatg ctggttaata ccagtaatta taatgaggga gtccaaaaaa caatgaccaa
      481 catcaccaag agaagtttag tagcagctgg cgttctggct gcgctaatgg cagggaatgt
      541 cgcgctggca gctgatgtac ccgcaggcgt cacactggcg gaaaaacaaa cactggtacg
      601 taacaatggt tcagaagttc agtcattaga tccgcacaaa attgaaggtg ttccggagtc
      661 taatatcagc cgagacctgt ttgaaggctt actggtcagc gatcttgacg gtcatccagc
      721 acctggcgtc gctgaatcct gggataataa agacgcgaaa gtctggacct tccatttgcg
      781 taaagatgcg aaatggtctg atggcacgcc agtcacagca caagactttg tgtatagctg
      841 gcaacgttct gttgatccga acactgcttc tccgtatgcc agttatctgc aatatgggca
      901 tatcgccggt attgatgaaa ttcttgaagg gaaaaaaccg attaccgatc tcggcgtgaa
      961 agctattgat gatcacacat tagaagtcac cttaagtgaa cccgttccgt acttctataa
     1021 attacttgtt cacccatcaa cttcaccggt gccaaaagcc gctatcgaga aattcggcga
     1081 aaaatggacc cagcctggta atatcgtcac caacggtgcc tataccttaa aagattgggt
     1141 cgtaaacgaa cgaatcgttc ttgaacgcag cccgacctac tggaacaacg cgaaaaccgt
     1201 tattaaccag gtaacctatt tgcctattgc ttctgaagtt accgatgtca accgctaccg
     1261 tagtggtgaa atcgacatga cttataacaa catgccgatc gaattgttcc agaagctgaa
     1321 aaaagagatc ccggacgaag ttcacgttga tccatacctg tgcacttact attacgaaat
     1381 taacaaccag aaaccgccat tcaacgatgt gcgtgtgcgt accgcactga aactaggtat
     1441 ggaccgcgat atcattgtta ataaagtgaa agcgcagggc gacatgcccg cctatggtta
     1501 cactccaccg tatactgatg gcgcaaaatt gactcagccg gaatggtttg gctggagcca
     1561 ggaaaaacgt aacgaagaag cgaaaaaact gctggctgaa gcgggttata ccgcagacaa
     1621 accgttgacc atcaacctgt tgtataacac ctccgatctg cataaaaagc tggcgattgc
     1681 tgcctcttca ttgtggaaga aaaacattgg tgtaaacgtc aaactggtta accaggagtg
     1741 gaaaacgttc ctcgacaccc gtcaccaggg tacttttgat gtggcccgtg caggctggtg
     1801 tgctgactac aacgaaccaa cttccttcct gaacaccatg ctttcgaaca gctcgatgaa
     1861 taccgcgcat tataagagcc cggcctttga cagcattatg gcggaaacgc tgaaagtgac
     1921 tgacgaggcg cagcgcacag ctctgtacac taaagcagaa caacagctgg ataaggattc
     1981 ggccattgtt cctgtttatt actacgtgaa tgcgcgtctg gtgaaaccgt gggttggtgg
     2041 ctataccggc aaagatccgc tggataatac ctatacccgg aatatgtaca ttgtgaagca
     2101 ctaatggcaa tacgtggggc aggagtgtcc tgctccacgg tgtctgattt ttatcgcatt
     2161 acagaaggca caggccagaa ggtagggcaa tgttaaaatt tattctacgt cgctgtctgg
     2221 aagcgattcc gacgctattt attcttatta ctatttcgtt ctttatgatg cgcctcgcgc
     2281 cgggaagccc ttttaccggc gaacgtactt taccgccaga agtgatggcc aatatcgaag
     2341 cgaaatatca tcttaatgat ccgatcatga cacagtattt cagctacctg aaacaactgg
     2401 cgcacggtga tttcggtcca tcgtttaaat ataaagatta ttcggtcaat gacctggttg
     2461 catccagttt tcccgtttct gccaaactgg gagccgcagc atttttcctt gcggtaatac
     2521 tgggtgttag tgctggcgtt attgccgcat taaaacaaaa caccaaatgg gactataccg
     2581 tgatggggct ggcaatgacc ggggttgtta tccccagttt tgtggttgcg ccattattag
     2641 tcatgatatt tgcgatcatt ttgcattggc tgccgggcgg tggctggaat ggtggggctc
     2701 ttaaattcat gatattgccg atggtggcgt tgtcactcgc ttatatcgcc agtattgcgc
     2761 gtattacccg tggctctatg attgaagtat tacactccaa ctttattcgt actgcccggg
     2821 cgaaagggtt acctatgcgg cggatcattt tacgccacgc attaaaacct gctctgttac
     2881 ccgtgctctc ctatatgggc cccgcatttg tcggcattat taccggttct atggtcatcg
     2941 aaaccattta tggtttgccg gggattgggc aactgttcgt taatggcgca ttgaaccgtg
     3001 actattcctt agtgttaagc ctgaccatcc tggttggtgc tttaaccatt ttgtttaatg
     3061 ccattgtcga tgtgctatat gcggttatcg acccgaaaat ccgttactga tactggagct
     3121 cgcgatgatg ttaagtaaga aaaacagcga gacgctggaa aatttcagtg aaaagctgga
     3181 ggtcgaaggg cgcagcttgt ggcaggacgc acgtcgacgt tttatgcata accgtgcggc
     3241 ggttgccagt ctgatagtgc tggtgctgat cgcgttattt gtaatcctgg caccgatgct
     3301 ttcgcagttt gcctatgacg atactgactg ggcgatgatg tccagcgccc cggatatgga
     3361 gtccggtcac tactttggta ctgactcatc cggtcgcgac ctgcttgtgc gcgttgcgat
     3421 tggcgggcgt atctcactca tggtcggtgt tgctgcggca ctggtggcag tggtcgtggg
     3481 gacactttac ggttcgcttt ccggttatct gggcggtaaa gtggattcgg taatgatgcg
     3541 tctgctggaa atcctcaact ccttcccatt catgttcttc gtcattttgc ttgtgacctt
     3601 tttcggtcaa aacatcctgc tgattttcgt ggcgattggc atggtttcct ggctggatat
     3661 ggctcgtatt gtgcgtgggc aaaccctgag tctgaagcgc aaagagttta ttgaggcggc
     3721 acaagttggc ggtgtatcga catcgggcat tgttattcgc cacattgtgc cgaatgtact
     3781 cggtgtggtg gtggtctacg catcacttct ggtgcccagc atgatcctct ttgaatcttt
     3841 ccttagcttc ctggggttgg gtacgcaaga gccgttaagc agctggggtg cattgctgag
     3901 tgatggcgcg aactcgatgg aagtctctcc atggttattg ttgttcccag cgggattcct
     3961 cgtggtgacg ctgttttgtt tcaactttat cggcgatggc ttgcgtgatg ccctcgaccc
     4021 gaaagatcgt taaggagtgc agccatgagc gtaattgaaa ctgcaactgt gccgctcgca
     4081 caacaacagg ctgacgcact gctgaacgtg aaagatttgc gtgtcacctt tagtaccccg
     4141 gacggcgacg tcacggcggt caatgatttg aatttttccc tacgtgccgg agaaacgctg
     4201 ggtattgtag gtgagtctgg ttcgggtaaa tcgcaaactg catttgcgtt gatgggcctg
     4261 ttggctgcca acggacgtat tggcggatcg gcaaccttca atgggcgtga aatcctcaat
     4321 ttgccagagc atgaactcaa taaactgcgc gctgaacaaa tctcaatgat ttttcaggac
     4381 ccaatgactt cgttgaatcc ctatatgcgc gtcggtgagc agttaatgga agtgctgatg
     4441 ctgcataaga acatgagcaa agctgaggcg tttgaagagt cggtgcggat gctcgatgcg
     4501 gtaaaaatgc cggaagcgcg taaacgcatg aaaatgtacc cgcacgaatt ttctggcggc
     4561 atgcgtcagc gagtcatgat tgcgatggca ttgctatgtc gacctaagct actgattgcg
     4621 gatgaaccca ctactgcgct ggacgtcacc gtacaggcgc agatcatgac gctattaaat
     4681 gaactgaagc gggaatttaa taccgccatc attatgatca cccacgatct tggcgtggtg
     4741 gcggggatct gtgacaaagt gctggtaatg tacgccgggc gcacgatgga atatggcaat
     4801 gcgcgcgatg tcttttatca acccgttcat ccttattcta tcggtttgct caacgcggtg
     4861 ccgcgtcttg atgcggaagg tgaaacaatg ttgaccatcc cgggtaatcc gccaaacttg
     4921 ctgcgattac cgaaaggttg cccgttccag ccacgttgtc cacatgcgat ggaaatttgt
     4981 agtagcgcac cgccgctgga agagtttacg cctggccgtc tgcgtgcttg ctttaaaccg
     5041 gtggaggaac tgttatgaat gctgtaactg aaggaagaaa agtcctcctt gaaatcgccg
     5101 atcttaaagt gcactttgaa atcaaagatg gcaaacagtg gttctggcaa ccgccgaaaa
     5161 cgctcaaagc cgtcgatggt gtcactcttc gcctgtatga aggggaaaca ttaggtgtgg
     5221 taggggaatc gggatgcggt aagtccacct ttgctcgcgc catcatcggt ttggtcaagg
     5281 cgaccgacgg tcatgttgcc tggttaggta aagagttgct gggcatgaag cccgatgaat
     5341 ggcgtgccgt tcgcagtgat attcagatga ttttccagga tccgttggca tcgctaaacc
     5401 cgcgtatgac catcggcgag atcatcgctg aaccactgcg tacttatcat ccgaaaatgt
     5461 cacgccagga agttcgcgag cgcgtgaagg cgatgatgct gaaagtcggg ttattgccta
     5521 acctgattaa ccgctatccg catgagttct ccggtgggca gtgccagcgt atcgggattg
     5581 ctcgtgctct tattcttgaa ccgaagctga ttatctgcga tgagccggtt tcggcgctgg
     5641 acgtgtcaat tcaggcgcag gtggtcaacc tgctccagca gctgcaacgt gagatgggat
     5701 tgtcattaat ttttatcgct catgacctgg ccgtggtaaa acacatttcc gatcgtgtgt
     5761 tggtgatgta tctcggccat gcggtagaac tggggaccta tgatgaggtc taccacaatc
     5821 cactacatcc ttacaccagg gcattgatgt cggcagtccc catacctgat ccggatctgg
     5881 agaagaacaa aaccatccag ttactggaag gggaattacc gtcgccgatc aacccgcctt
     5941 ccggttgtgt tttccgtacc cgttgcccga ttgccggtcc ggagtgcgcc aaaacacgtc
     6001 ctgttctgga ggggagtttc agacacgccg tttcttgcct gaaagtcgat ccgctttaaa
     6061 cacaataagg gctgacagtt gtcagccctt tttcacgccg aaagcgatta tttattcccg
     6121 ccagatgata tggcaaagtt tgtgatcttt ttcgcggcat aacagaatgc gggcgaaaac
     6181 atcgttgatt tcaccatctt cactgtccgc cagaccaatc accacttcgg caaaaaagtc
     6241 tgggttcaaa tcgaaatcaa catgttcctg ccagtcttcc gccggatcga ataactccgc
     6301 gccgccgcgc tcttcaaact gaagattgaa cagcagaaca tcagctggat cgagattgtc
     6361 ggcagccagt tcgagaaaaa tatcgtaggc ctgctcaagc gtttcatctt cagtcaggcg
     6421 attgtttaga tccatatcca tagtcactac ctgtttaacc tctgttggcg acgttttaca
     6481 gcaacggact gaagaagtaa aacagtcgct cggcgacacg ttgccataat ggacgtttta
     6541 gccataaacg ggcatcgagc agacgtgaac gcgaaatata atcgtcctga acggcggcaa
     6601 ggtctgcacc aaaaccttta tcgtcgattg ccagggtaat ctcgaaattc agccacagac
     6661 tacgcatatc aaggttaacg gtgccaacca gacttagttc gccatcgacc agcacgctct
     6721 tggtatgcag taacccgcct tcaaactgat aaattttaac cccagcagca agaagttccg
     6781 taaagaatgc gcgactggcc cagccgacca gcatcgagtc attttttcgc ggaaggataa
     6841 tactgacatc caccccgcgc tgcgccgccg tgcaaatcgc atgaagtaaa tcatcgcttg
     6901 gcacaaagta gggcgtggtc atgatcaaat attcacgcgc cgaataagcc gcagtcaata
     6961 atgcctggtg aatgagatct tccggaaagc cggggccaga agcaattgtg tgaatggtgt
     7021 gaccgctggc ctgttcaaac ggcataatat tgacatctgg tggtggcggc agaatacgtt
     7081 ttccggtttc aatctcccag tcgcaggaat aaataatccc catcgcggtg gcgatggggc
     7141 cttccatacg cgccatcaga tcaatccatt gccctacgcc cgcatcttgt ttgaagtagc
     7201 gaggatcgac catattcatg ctgccggtgt acgcgatgta attatcgatc atgatcatct
     7261 tgcgatgttg gcgcaggtcc atacggcgta aaaacacacg catcagattg acctttaagg
     7321 cttcgaccac ttcaataccg gcattacgca ttagctcggg ccacgggctg cggaaaaaag
     7381 ccacactccc ggcggagtcg agcatcaatc ggcaatgaat accacgccgt gcagccgcca
     7441 ttaatgattc agccacctgg tccgccatgc cgccgggctg ccagatataa aacaccatct
     7501 caatattatg gcgcgcgagc tggatgtcgc ggattaacgc ctgcatcaca tcatctgact
     7561 cggtcatcag ttgtagctga ttccctttga ccccagcgat cccctgacga cgctcgcaaa
     7621 gcttgaataa tggcgcagcg acactgctat tttcttcggc gaagatatgc ttacaggctt
     7681 taaggtcgtt aagccatttt gcggtggaag gccacatcgc tctggcgcgc tcagcgcggc
     7741 gtttgcctaa atggagctcg ccaacggcaa gataggcaat aattccgact aacggcagaa
     7801 tgtaaataat caacagccag gccatcgcgg agggaactgc gcgtcgtttc attagaatgc
     7861 gtaaagttac gcctgcaatg agcaaccagt atcccagaat ggccaaccaa ctcaccaacg
     7921 tataaacggt tgtcatagat tagaaatcct tttgaaagcg cattgttatg agtttacgca
     7981 tatggattca tctggcaaat aaaaacgcgg gaaaagcact ggtcagcgtc gggggtgggg
     8041 gtataatgac cattctgtta ttgcatagag tagttaacat gaagcggagt agaacggaag
     8101 tggggcgctg gcgcatgcag cgtcaggcta gccgacgtaa atcgcgttgg cttgaggggc
     8161 aatcgcgccg aaatatgcgt atccacagca tcaggaagtg cattctaaac aaacagcgta
     8221 actcgttatt gtttgcgatc tacaatatct aaatatactg ggcaccattg cggtgcccaa
     8281 taagtttact ctttcgtctt taaactctat ttttgcactg attctttact gtcagctttc
     8341 gtttcttgtt catcgttatc gctgaaaatg ccatgaccgg agttcaacaa cattgaaacg
     8401 agcatatcat tattaatctc ttcaccattt aaaactcgcg ccagaatttc gctgccaaac
     8461 agttgcggtg agagaatgat atccggatgt accatcttaa tcttattcag gtttttgcta
     8521 tcactgacgg cgagaactgt tttgacatca ctgctcatat cttttgccga gagtacaaca
     8581 aacgcgttat ctgcatcgtt atcactcagc gccagaatgg ctcggcagcg atcgattccc
     8641 gctttcttta atactgaact gtcattactg tcaccgggga taacatcagc gttatcgcct
     8701 aagcgttgct caagttgctt gatatcatct tcaggcaagt tgctgataac cgttacgttt
     8761 tgtccgcgtt gattcagttg caggatcgta ttgatggcga gaatcgaatg tccgcaaaca
     8821 ataaaatgat ctttacgatg cattgtatga ttgtttcctt ttacaagttt gttgaatccc
     8881 ccgcggataa gcggaccaaa aattgaggtc atagatgtgg caaaaacggt aatgccggaa
     8941 ataatgaccg aaatagtgaa caatcgtgct gattcagaaa cagggacaat atcgccatag
     9001 ccgacggttg acatggtttc tatcgaaaaa tagaacgcgg tcatcaaact ttctattcgc
     9061 ggattaaaac cttcgcttaa ataaagcgca ccgtaggttg agtaaaacag taatgtcgtg
     9121 aaactaataa atgcaaaaat tgtcccggct gcggcgctac tgtgggagaa gtctttgcgc
     9181 agtatcagca aaaacaccag cgtaaaaatg caaaatccaa tactaaattt cagccaggga
     9241 taaaaatgca gggtgtaaat tagcgctatc aacaacaaaa tgatactgat tgcccatgcg
     9301 agctttgccc ggaacagtaa gccaatggag ttcaggacaa gaaagacgcc gagcataaat
     9361 agtggggcgt ttgccagcaa ggagagatcc aactctgaaa atgctttgat atggaaaata
     9421 tcaagcaaat tgaccgacat accatagatt gttttaaaaa taagcaatcc atttaaaaag
     9481 acggccagcg cgagaatgtc gtgccgtagg gtcacccata aatttgttgc tgtttgtttg
     9541 aatgtagccc agtgactcac tgatacctct gtaaccacaa ttttgtttaa ttatcttagc
     9601 gtgcacattt tctgaacata catgcagcgc gtgaatgtgt taaacaatag tagatgactt
     9661 tatcggcagt aatcaaaggt tttgagcgag caactctgcg tcaggaaagg gcattttcct
     9721 gaatatgctc acatcatata aagaaataca gataaagtta ttatctgctt gtggtagtga
     9781 atgcactgac cggctataag gaaaggccaa acaagaacac ggttgcaaaa accgtgccct
     9841 taaatattga atctctattc agaacacttt cttaaatggt ttcactgaaa cgtgttcata
     9901 gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc tgagctgctt ccagcgattc
     9961 aaattcagca ataacggttg agccagtaaa tcccgcagcc cctggatcgt tactgtctac
    10021 cgctggcatt ggaccagctg tcaacaaacg accttcatca tgcagtaact gtaaacgtgc
    10081 taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta tcagctttgt cttgagcgta
    10141 aataacatac aacacgggca caactccttg ttcgggaaag ttgtaaagta cgttatttga
    10201 aagggcgaag atctgcaacg gaaagatgat gtctttgtta agaccatgca taaattaagg
    10261 tcaattacgc caaaaatgac attttcactg attctgatcg tcttgcctta ttgaatatga
    10321 ttgctatttg catttaaaat cgagacctgg tttttctact gaaatgatta tgacttcaat
    10381 gacccttgat ttacctcgcc gcttcccctg gccgacgtta ctttcggtct gcattcatgg
    10441 tgctgttgtg gcgggtctgc tctatacctc ggtacatcag gttattgaac tacctgcgcc
    10501 tgcgcagcca atttctgtca cgatggttgc gcctgctgat ctcgaaccgc cacaagccgt
    10561 tcagctgcca ccggagccgg tggtagagcc agaaccggaa cctgagccga tccccgaacc
    10621 gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg aaacctaagc caaaaccgaa
    10681 gccggtgaaa aaggtacagg agcagccaaa acgcgatatc aaacccgtag agtcgcgtcc
    10741 ggcatcaccg tttgaaaata cggcacccgc gcgccccaca tcaagtacgg caacggctgc
    10801 aaccagcaag ccggttacca gtgtggcttc aggaccacgc gcattaagcc gtaatcagcc
    10861 gcagtatccg gcacgagcac aggcattgcg cattgaaggg caggttaaag ttaaatttga
    10921 cgtcacgccg gatggtcgcg tggataacgt acaaatcctc tcagccaagc ctgcgaacat
    10981 gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt tatgagccgg gtaagccagg
    11041 cagtgggatt gtggtgaata tcctgtttaa aattaacggc accaccgaaa ttcagtaagc
    11101 agaaagtcaa aagcctccga ccggaggctt ttgactatta ctcaacaggt aaggcgcgag
    11161 gttttccttc aggatcaacc gcgacatact taaataatgc ttctgtcgct ttatagcgtt
    11221 gcccgattgg ttcagacgct acttttttca cccacacttc aatattaatg ctgaccgatg
    11281 tcgtcccttt ctggacacag cgtgcatagc agcacaccac atcgccgacc gcaaccggcc
    11341 gtaagaaagt cattccttca acccgcacag tcactacgcg accgtgggca atttcttttg
    11401 ccagaatagc gccgccaata tccatttgtg acattaacca accaccaaag atgtcaccat
    11461 tggcattggt atcggcgggc atggctaaag tacgtaaaac aagatcgccc tgagggacgt
    11521 tatgtgttgt agacatggta aaaccgactt aaaaggaaaa tcacaggcgc gatgctacta
    11581 tgattttcct gaggagaaca gaggagatac agcgccagcc ccgaaggact ggcagtctgg
    11641 ttaggattta tcttcctgcg gcatgtggcg gtagatatag ataccgctta acaatgtgaa
    11701 gattaaggta agggcggtca ggccaaagac tttaaagttg acccaaatat tttgcggcag
    11761 ccagaatgcg atgtagatgt ttgccagacc gcaaaggata aagaaaacag cccaggccag
    11821 attcagcttc gaccataccg gttgcggcag cgtgagttct ttacccagca tccgctgaat
    11881 tagcggcttt ttcatcaccc attggctgac taacagggct cccgcaaata gagcataaat
    11941 gactgtaacc ttccatttaa taaactcatc attgtggaag aacagcgtca agccaccgaa
    12001 gacgaccacc agaacaaaag tgatcagggc catcttctca accttacgaa agcgaaccca
    12061 gctatagata agcacaatcg ccgtggcgac gatcagcgcc gcagtagccg catagatgtc
    12121 ataaatcttg taaaacgcga aaaagacaac cagcggtaaa aaatcaagaa actgcttcat
    12181 tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa tcaacatata caggcggaac
    12241 agatagatga gcaatatggc agaaatcagg ttgctcaagg tgttcgccag aacggcacca
    12301 atttccgggg ttaatgcggc aaaagaagag gcaaaaagca gcaacagtgt ttttgccagc
    12361 aaccagctca gtactgcggg tgccaccaga cgcatattcg cccaagtcag ccgcatactg
    12421 ctacgcatcg aggcaaaaat gcccattttg tcctgtacca gcatcaccgg tgccagggcc
    12481 agtaaaatgg ccatgataat tcccggaacg accaccagca tgatgccaat ctgtactaaa
    12541 agggtagtca gaaaaatcag aataaataac ttgggcaata tcggcgcact ggcaccaata
    12601 gctcgcagcg cactgactct ctgacccgca gacaccagct ggataattaa tattacgcct
    12661 ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg acgccgctga agcctgcagc
    12721 aaaatttgtt gctgttccgg tgacatattc tgaaccaggt cgaacaaccc actactgccg
    12781 ctaacgggca cgccgtcatt gagctgcgca agctgtgcat cactgggtga gaaaacatgc
    12841 cctaacacca ctgtgataaa cgcacatagc aacgatacca acagaatggt cataaattga
    12901 ttacggaaga aatttccggt gtcacggtat acggactgcg ccgtgataga catgcactct
    12961 ccttgagttt tatgcaggtg ttaattagcg ggcaattgta ccctggttaa gcctcgggtg
    13021 gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa gcggaggtaa ttgctggcct
    13081 tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa tatttgcgta gagaaaatat
    13141 ttaaaaattg atttaaatca cattaaccag gattctcaat gcaacttcta aattaatcca
    13201 gatcaataaa tggtgaatta tcatatgtaa tgtgatctat gtaggatcat ttgttactcc
    13261 aatgtaggta tattcgtcac gtttttataa ccataacgac ggagcggata tgaaaaagtt
    13321 aacagtggcg gctttggcag taacaactct tctttctggc agtgcctttg cgcatgaagc
    13381 aggcgaattt tttatgcgtg caggttctgc aaccgtacgt ccaacagaag gtgctggtgg
    13441 tacgttagga agtctgggtg gattcagcgt gaccaataac actcaactgg gcttgacgtt
    13501 tacttatatg gcgaccgaca acattggtgt ggaattactg gcagcgacgc cgttccgcca
    13561 taaaatcggc acccgggcga ccggcgatat tgcaaccgtt catcatctgc cgccaacact
    13621 gatggcgcag tggtattttg gtgatgccag cagcaaattc cgtccttacg ttggggccgg
    13681 tattaactac accaccttct ttgataatgg atttaacgat catggcaaag aggcagggct
    13741 ttccgatctc agtctgaaag attcctgggg agctgccggg caggtggggg ttgattatct
    13801 gattaaccgt gactggttgg ttaacatgtc agtctggtac atggatatcg ataccaccgc
    13861 caattataag ctaggcggtg cacagcaaca cgatagcgta cgcctcgatc cgtgggtgtt
    13921 tatgttctca gcaggatatc gtttttaatt ccacaaaaaa acgaccccgt aatatacggg
    13981 gtcaataagg acatggcata aagtgtatta tttcttcgct tctacgccgt cagtttcaga
    14041 gcgaattaaa aatttctcag ttgtttgcgg aatatgctga atcagccagt cggccatttg
    14101 cttttcctca tttaaaatcg cttcgatggt tggaattgaa gctgtatcac cggcattttt
    14161 tgctgctgct aatagtgagg tgtaacaggc gatttcaaat tgctcgaaga catatccgct
    14221 aatagagcct ttgactattt catcagaagg gaatatacca ccgattgact gcccaagcgc
    14281 agccatttta ctcatggaat ctttaatgac tgaacgtgaa atgtcattac gatcaagaat
    14341 agtttccaat tgaacaatct ggtttttggt ttcactaaga tgttgttcaa tacgagcgcg
    14401 tagttcagga taattatcta tacggctggc catggattca agcatagatt cggcttgctt
    14461 ttccattgcg tgggcgtcac gtaaccagtc atgataatgt tcaatacgat tcatgaaaat
    14521 gtcctcttta taaaatgact aaaagttaaa gttcatattt caggctttat tttcggcttt
    14581 cttatttacg ttattaaggg ccagatcagt cagtttgatg tcggtggcct tttcttcttc
    14641 cagggtctct ttcagaagct tcgctgcttt acgataacct aattgttcag ccagcgtcgc
    14701 taatgtcccg taactggcaa tctcataatg ctcgactttc tgtgctgcgg caatcagtgc
    14761 ggcatcacgc acttcgtttt tctcggtact ttcgatgacc tcattagctt cttcaataag
    14821 accttccatt gccacacatt tcatgcgctt aattttcaga ttcgattctg attccacaac
    14881 ttgatcaata cgttcaatct gtccatgtgt ttcctcgagg tgcgcatgaa aagcctgact
    14941 taatttttcg tttgatgttg ctcttgcgag ttttgccagt gcccgggtta attgtttttc
    15001 tgcgctgtag gtatctgaaa gcaggtgaat aaatacatct tcaatggtct tcatattcat
    15061 atttttctcc agtgaaatca ctgcgagcca tacggcccgc agatgcaatt atctttatca
    15121 gctgatatta ataattaacg gtgaatcagg atttgcctga tttattacca ccgctttgtt
    15181 gaccgccttt tttacccgct tcagatgcgc gttgcggatc atttttaaaa ttaccgccgc
    15241 tatgctgacc gcctttacgg cctgcgtcgg atgccttctc acggtcttcg gcgaaatttc
    15301 ctgaaccacc acgatgttcg gccatgttat ttctcccgtt gcgttgcatt gtttcattaa
    15361 tatgagtgtt gtgtgtcgac actcattaaa attagtcgct aatgagaatt agtcaaatta
    15421 agcgcaacga gaagatagag ggaaaatata ttttgaggaa cattctg
//
LOCUS       DAPHDX010000101        15333 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPHDX010000101 DAPHDX010000000
VERSION     DAPHDX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.102"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..680
                     /gene="zinT"
                     /locus_tag="RH058_003244"
     CDS             30..680
                     /gene="zinT"
                     /locus_tag="RH058_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDV3138795.1"
                     /translation="MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1023..1553
                     /locus_tag="RH058_003245"
     CDS             1023..1553
                     /locus_tag="RH058_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV3138796.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2123..2212)
                     /locus_tag="RH058_003246"
     tRNA            complement(2123..2212)
                     /locus_tag="RH058_003246"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2176..2178),aa:Ser,seq:cga)
     gene            2306..3103
                     /gene="mtfA"
                     /locus_tag="RH058_003247"
     CDS             2306..3103
                     /gene="mtfA"
                     /locus_tag="RH058_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV3138797.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRTCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANNTDSFSATNVH"
     gene            3204..3279
                     /locus_tag="RH058_003248"
     tRNA            3204..3279
                     /locus_tag="RH058_003248"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3237..3239,aa:Asn,seq:gtt)
     gene            3591..10667
                     /gene="yeeJ"
                     /locus_tag="RH058_003249"
     CDS             3591..10667
                     /gene="yeeJ"
                     /locus_tag="RH058_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HDV3138798.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLCRLTAGICLVTQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDAKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSVTMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASKFSISNGGKGMTDSNGVAIASLTGTLAGTHMIMARLANSNVSDA
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVNFTMPQDV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKASA
                     QVVLQISKDEITGNGVDSATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGETTSLY
                     IEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQLTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKVITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTAKLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEI
                     LVTSTEVGLKTVSASLTDKPTEVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVK
                     AHVNDQFGNPVAHQPVTFSAEPSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKAS
                     LPNGASLEKQLEAIDEKLTLTASSPLIGVYAPTGATLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNTDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGD
                     YTDSAELRLVLHDISGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTG
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSY
                     VYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHCSSRGIGSLYSEWGDM
                     GHYTTDAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(10929..11981)
                     /locus_tag="RH058_003250"
                     /pseudo
     CDS             complement(10929..11981)
                     /locus_tag="RH058_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
     gene            12296..13612
                     /gene="shiA"
                     /locus_tag="RH058_003251"
     CDS             12296..13612
                     /gene="shiA"
                     /locus_tag="RH058_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV3138799.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGTLIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            13714..15168
                     /gene="amn"
                     /locus_tag="RH058_003252"
     CDS             13714..15168
                     /gene="amn"
                     /locus_tag="RH058_003252"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV3138800.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4189 a         3627 c         3565 g         3952 t
ORIGIN      
        1 catatacatt aactctggag gaaactgttt tggcgattcg tctttacaaa ctggctgttg
       61 ctttaggtgt ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca
      121 aacccttaac agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac
      181 aaaaccgaac gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa
      241 gtggcaaact tgaccccgtc tttcagaaga aagcggatgc agataaaact aaaacatttg
      301 ctgaaattaa agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg
      361 aggacggcat tgttgaattc catagaaata atgaaacaac atcgtgtaaa tatgattacg
      421 atggatacaa aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat
      481 gtaaagatcc tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac
      541 cacgaaaatc atctcatttt cacattttta tgggtaatga ttcacagcaa tctttattaa
      601 atgaaatgga aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg
      661 aggaaatgat gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtt
      721 atcataactg ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg
      781 cttatcacta tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac
      841 ggattccata cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata
      901 ttggctggtt acaatatgca ctatcaattt tttaaataac aaattattaa aaccacatct
      961 gtataaggaa tttttaaggt tcgtgggata gcttgactgt gaaaatcaca ggagctacaa
     1021 aaatgaaccg attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg
     1081 caataaccta tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc
     1141 tgctgatgcg agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac
     1201 gcctgattat aaaacaccgt tatagtgatc cttctattgt gccaccgcca cctgcctggc
     1261 aaatgaaagc ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc
     1321 tgctggggat tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg
     1381 tgtctccctt tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa
     1441 cctgggcaaa tataggctac tttttaatcg cagctcacgc tggcgcagca ctctttcatc
     1501 actacattca gaaagataat actctgttac gaatgatgcc tcgccgcaaa taagtcgatc
     1561 cccttacaag gaaatgtgaa ccatggacat caacgcgtca taaatgttta cgcaagtgca
     1621 caatgcaccc tttccactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt
     1681 aagacttatc aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg
     1741 atacaaaggc tttcaaaaaa gctgcgaaat tcaacaactc ttggaaatta tggactttgt
     1801 cccgcggctt taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct
     1861 acgcaacttc agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag
     1921 aataatggtg agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc
     1981 tgtcagatcc atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg
     2041 atctaaccct tcaagaatat tctacgattg ttctgtttag gaaaagcaag gcgggaagtc
     2101 gggagataag tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg
     2161 cccctactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     2221 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     2281 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     2341 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     2401 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     2461 tgttccttta cagggctttg aactggattc attaagaacc tgccggatag cacttctatt
     2521 ttgcctaccc gttctggaat taggactgga atggctggat ggttttcatg aagtcttaat
     2581 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     2641 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     2701 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2761 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2821 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2881 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2941 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     3001 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     3061 cgctaataat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     3121 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     3181 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3241 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttctt
     3301 agtgcaatgt cactaacctt agttgaacat tgttttttaa cggatagcgg gtttttaaca
     3361 tcttaagcgc cctcgaactt tatggttgag ggcgttttgc tatggcgtca tcaccatttt
     3421 cccctcgatt ataaaacttg agtaattcag tagtctcccc tcttgcaact cacacccaaa
     3481 actgcctaac gaaaagttat taattttcaa tcatattgct atcaggattt acattttttc
     3541 gctgtgctag aaagggcgca tttatgttag ctcgttcagg gaaggtaagc atggctacga
     3601 agaagagaag tggagaagaa ataaatgacc gacaaatctt atgcgggatg ggtatcaaac
     3661 tatgccgctt aactgcgggt atctgcctgg taactcaact tgtgttccct atggctgcgg
     3721 cagcacaagg tgtggtaaac gccgcaaccc aacaaccagt tcctgcacaa attgccattg
     3781 caaatgccaa tacggtgccc tacacccttg gagcgctgga atcggcccaa agcgttgccg
     3841 aacgtttcgg tatttcggtg gctgagttac gcaaactcaa ccagtttcgt acgtttgctc
     3901 gaggttttga taatgtccgc cagggtgatg aactggatgt cccggcacaa gttagtgaaa
     3961 aaaaattaac cccgccgccg ggtaatagca gtgacaacct cgagcaacag atagccagta
     4021 cttcacagca aatcgggtct ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata
     4081 tggcgcgtgg atgggcctct tctcaggctt caggcgcaat gacagactgg ttaagccgct
     4141 tcggtaccgc aagaatcacg ctgggcgtgg atgaagattt tagcctgaag aactcccagt
     4201 tcgattttct ccatccgtgg tatgaaacgc ctgataatct ctttttcagt cagcatactc
     4261 tccatcgtac tgacgagcgt acgcagatta acaacggctt aggttggcgt catttcactc
     4321 ccacatggat gtcgggcatc aacttctttt tcgaccacga tcttagccgt taccactccc
     4381 gcgccggcat tggcgcggag tactggcgcg actatctaaa attaagcagt aacggctatt
     4441 tgcgactgac caactggcgc agcgcacctg aactggacaa cgattatgaa gcacgcccgg
     4501 ccaatggctg ggatgtacgc gcagaaagct ggctacccgc ctggccgcac cttggcggta
     4561 aactggtcta tgaacagtat tatggcgatg aagtggccct gttcgataaa gacgatcggc
     4621 aaagtaatcc tcatgccata accgctggac ttaactatac ccccttcccg ctgatgacct
     4681 tcagcgcgga gcaacgccag ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt
     4741 ttacctggca acctggcagc gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac
     4801 ggcgtagcct tgcaggcagc cgttatgatc tggtggatcg caacaacaat atcgttctgg
     4861 aatatcgcaa aaaagaactg gttcgcctga ccctgacaga ccccgtgaca gggaagtcag
     4921 gagaagtgaa atcactggtt tcgtcgctac aaaccaaata tgccctgaaa ggctataacg
     4981 tcgaagccac cgcactggaa gctgccggtg gcaaagtggt cacaacgggt aaagatattc
     5041 tggttaccct gccggcttac cggttcacca gtacgccaga aaccgataac acctggccga
     5101 ttgaagtcac cgccgaagat gccaaaggca atttgtcgaa tcgtgaacag agcatggtgg
     5161 tcgttcaggc acctacgcta agccagaaag attcctcggt atcgttaagt acccaaacat
     5221 tgaacgcgga ttcccattca accgccacac tgacttttat tgcgcatgat gcagcaggta
     5281 atcctgttgt cgggctggtg ctctcgacgc gtcacgaagg tgttcaggac atcacccttt
     5341 ctgactggaa agataatggt gacggaagct atacccagat cctgaccaca ggagcgatgt
     5401 ctggcacgct gacgctgatg ccacagctga atggtgtgga tgcggctaaa gcccccgccg
     5461 tggtgaatat catttctgtt tcgtcatccc gaactcactc gtcaattaag attgataagg
     5521 accgttatct ctccggcaat cctatcgagg tgacggtaga actgagagat gaaaatgaca
     5581 aacctgttaa ggaacaaaaa cagcaactga ataacgcagt cagcatcgac aacgtgaaac
     5641 caggagtcac tacagactgg aaagaaaccg cagatggcgt ctataaggcg acctataccg
     5701 cctataccaa aggcagtgga cttactgcga agctattaat gcaaaactgg aatgaagatt
     5761 tgcataccgc tggatttatc atcgacgcca acccgcagtc agcgaaaatt gcgacattat
     5821 ctgccagcaa taatggtgtg ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg
     5881 tcgctgatga aggaagcaac ccaatcaatg atcataccgt cacgtttgcg gtattaagcg
     5941 gatcggcaac ttccttcaac aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga
     6001 cttttgatct gaaaagtagt aagcaggaag acaacacggt tgaagtcacc cttgaaaatg
     6061 gcgtgaaaca aacgttaatc gtcagttttg tcggcgactc gagtaccgcg caggttgatc
     6121 tgcagaagtc gaaaaatgaa gtggttgctg acggcaatga cagcgtcaca atgaccgcga
     6181 ccgtccggga tgcaaaaggc aacctgctca atgacgtcat ggtcactttc aatgttaatt
     6241 cagcagaggc gaaactgagc caaaccgaag tgaatagcca cgacgggatc gccacagcta
     6301 cgctgaccag tttgaaaaat ggtgattata gggttacggc ctctgtgagc tctggttccc
     6361 aggctaatca acaggtgaat tttatcggtg atcaaagtac tgctgccctg accctcagtg
     6421 tgccttcagg tgatatcacc gtcaccaaca cagctccgca atatatgact gcaaccttgc
     6481 aggataaaaa tggcaaccca ctaaaagata aagaaatcac cttctctgtg ccaaacgacg
     6541 tcgcaagtaa gttctcgatt agcaacggag gaaaaggcat gacggatagt aacggggttg
     6601 caatcgcctc cctgaccggc acgttagcgg gcacgcatat gatcatggct cgtctggcta
     6661 acagcaatgt cagcgatgca cagccaatga cgtttgtggc ggataaagac agagcggttg
     6721 tcgttttgca aacatcgaaa gcggaaatca ttgggaatgg cgtggatgag acaactctga
     6781 cagcaacagt gaaagatcca tcgaatcatc cggtggcggg gataacggta aacttcacca
     6841 tgccacagga cgttgcggca aactttaccc ttgaaaataa cggtattgcc atcactcagg
     6901 ccaatgggga agcgcatgtc acgctgaaag gtaaaaaagc gggcacgcat acggttaccg
     6961 caacgctggg taataacaat accagtgatt cgcagccggt aacatttgtg gcggacaaag
     7021 cctcggctca ggttgtcctg cagatatcaa aagatgagat cacaggtaat ggcgtcgata
     7081 gcgcaacgct aactgcaacg gttaaagatc agttcgacaa tgaggtgaat aatcttccgg
     7141 taacattcag ctcagcctct tcaggactca ccctgacccc gggagtaagt aataccaacg
     7201 agtctggcat cgcgcaggcc actctcgcag gcgttgcctt tggtgagaag acggttactg
     7261 catcactggc taataatggt gccagcgaca acaaaactgt gcattttatt ggcgacacag
     7321 cggcggcaaa aattatcgag ttgacgcctg tcccagacag cataatcgcc ggtaccccgc
     7381 agaacagctc cggcagcgtc atcaccgcca cagtcgttga taataatggc tttccggtga
     7441 aaggtgtgac tgtgaacttc accagcaacg cagcgacagc cgaaatgacg aacggcggtc
     7501 aagccgtgac gaacgaacag ggtaaggcta ccgtcactta taccaatacc cgctcctcga
     7561 tagaatcagg agcgagaccg gataccgttg aggccagtct ggaaaatggt agctccacgc
     7621 ttagcacatc aattaatgtc aacgctgatg cgtctacggc acatctcacc ttgctacagg
     7681 cactttttga tacagtctcc gcaggcgaga caaccagtct gtatattgag gtgaaggata
     7741 attacggcaa cggtgtcccc cagcaggagg taaccctcag cgtttcacca agtgaaggcg
     7801 tgacccccag taataacgct atatatacta ccaaccacga cggcaatttt tacgcaagct
     7861 ttaccgctac aaaagccggg gtttatcaat tgacggcaac cctcgaaaat ggcgattcga
     7921 tgcaacaaac agtgacctat gtgccgaacg tcgcgaatgc tgaaatcacg ctggcagcct
     7981 cgaaggatcc ggtgattgcc gacaataacg atctcacgac actaacagca acagtcgctg
     8041 atacagaggg caatgcgata gccaacactg aggtaacatt tactctgccg gaagatgtga
     8101 aggcgaactt cacgctgagc gatggcggta aagtgattac tgatgctgaa ggcaaagcga
     8161 aagtcacgct gaaaggtaca aaagcaggcg ctcatactgt tacagcatcg atgactggcg
     8221 gtaagagtga gcagttggtg gtgaacttta ttgcggatac gctcactgca caggttaatc
     8281 ttaacgttac cgaggacaat tttatcgcta ataacgtcgg gatgaccagg ctgcaggcaa
     8341 cagtgactga tggaaacggc aacccgttag ccaatgaggc ggtgacattc acgctaccgg
     8401 cagatgtgag cgcaagcttt actctcggac aaggcggttc cgccattact gatatcaacg
     8461 gcaaggctga agttacactg agcggtacaa aatccggcac ctaccccgtg acagttagcg
     8521 tgaacaatta tggtgtcagt gatacgaaac aggtgacttt gattgccgat gctggtaccg
     8581 caaaactagc ctccttaacc tctgtatact cattcgtcgt cagcacgacc gagggcgcaa
     8641 ccatgacggc aagcgtcact gacgctaacg gcaacccggt agaaggcata aaagttaatt
     8701 tccgcggaac ctccgtcacg ctaagcagca ccagcgttga aacggatgat cggggtttcg
     8761 ctgaaattct tgtgacaagc accgaggtcg gactgaaaac agtttcagcc tctctgacag
     8821 ataaacctac tgaagtcatc tcgcgattac tgaatgccag tgcagatgtt aattctgcga
     8881 cgattaccag tctggagata ccggaaggtc aggtaatggt cgcacaagac gtagcagtta
     8941 aagctcacgt taacgaccag tttggcaacc cggttgcgca tcaacccgtg acattcagtg
     9001 cagagccatc ctcgcaaatg atcatcagcc agaatacggt ctctactaat acgcagggtg
     9061 tagccgaggt caccatgacg cccgaaagaa acggttcgta tatggtgaaa gcatccctgc
     9121 cgaatggagc ctcacttgag aaacaactgg aggctattga tgaaaaactg acactcacgg
     9181 cgtccagtcc gcttatcggt gtctatgccc ctacaggcgc tactctgacg gcaacgctaa
     9241 cctctgcaaa tggcactcca gtggagggtc aggtcatcaa ctttagcgta acgccagaag
     9301 gggcgacgtt aagtggcgga aaagtgagaa ctaactcttc aggtcaggct ccagtcgttt
     9361 tgaccagcaa taaagtcggt acatatacgg tgactgcatc tttccataac ggcgtaacaa
     9421 tacagacaca gacaaccgtg aaagtcactg gcaactcaag caccgcccat gttgctagct
     9481 ttatcgctga tccatcgact atcgccgcca ccaacactga tttaagtacc ttaaaggcaa
     9541 cggttgagga tggcagtggt aacctgatcg aaggtctcac tgtgtacttc gccttaaaaa
     9601 gcggctctgc cacattaacg tcattaacag cggtgaccga tcaaaacgga atcgcgacaa
     9661 caagcgtgaa aggagcgatg acaggtagcg tcacggtaag cgcagtcacg accgctggtg
     9721 gaatgcaaac agtagatata acgctggtgg ctggcccggc agacacctcg cagtccgtcc
     9781 ttaagagcaa tcggtcatca ctgaaagggg actataccga tagtgctgaa ttacgtcttg
     9841 ttctgcacga tatatcaggc aatccgatca aagtttctga agggatggaa tttgtgcaat
     9901 caggtactaa cgtgccctat ataaaaatta gcgcaattga ttacagtcta aatatcaacg
     9961 gtgattacaa agccactgtt acaggaggcg gagagggtat cgcaacgctg atccctgtat
    10021 tgaatggtgt tcatcaagct ggtctgagta ccacaataca attcactcgc gcagaagaca
    10081 aaataatgag cggtacagta tcagtcaatg gtactgacct accgacaact acattccctt
    10141 cgcaggggtt caccggggcg tattatcagt tgaataatga caactttgcc ccaggaaaaa
    10201 cggcggctga ttatgagttt tcaagctctg cctcctgggt cgatgttgat gctaccggta
    10261 aagtgacatt taaaaatgtc ggcagcaatt cggaaaggat tacggcgacg ccaaaatcag
    10321 gaggccctag ctatgtatac gaaatccgtg tgaagagttg gtgggtgaac gccggcgagg
    10381 ctttcatgat atacagcctt gctgaaaatt tttgcagcag caatggctac acgctcccca
    10441 gagcaaacta tttaaaccac tgtagttccc gaggcatcgg gtcactgtac agtgaatggg
    10501 gagatatggg gcattacacg actgacgctg gttttcaatc aaatatgtat tggtcatcta
    10561 gtcccgcaaa ctcaagcgaa caatacgtag tttccctggc aacaggtgat caaagcgtat
    10621 ttgaaaagct tgggtttgct tatgcgacat gttataaaaa cctgtgattt tccgttataa
    10681 tttcttaaag agtgttccat tcggggcact cttttttaca ttccctctac agaactagcc
    10741 cttctctcac agaaaatgaa taaataaaaa tgcggcaccg ccagaatcgc gttcgatgtg
    10801 actatgatca ttttcggtga gtactgcttt gatctaaagg ctggttttct gtgatgattg
    10861 accagcctca gagtattgat gacaccaccg ttcataacag atcggcatta ctatgcataa
    10921 gtactgtctc atattctcgc caaaacactt tttaatgcct ttgcgaattc atccagactt
    10981 atattgtcaa ttttttgccc aacgacgact gactccgaga catcggccat tgcgggccat
    11041 cgcgttttgt aatcagcata aattgcaaca acaggggtat tataggcact tgccatatgt
    11101 aatattgagg tgtcaggagt tattgcgata taagcatctt ttatgattgc tgctgaacgt
    11161 ttaatcgagg gtggtaagga taaacggtag acattattat aacaatccac taatgccctg
    11221 gctttatctt cacctttggg tccatgaacg atcactattg gtatatctgt ttcactttaa
    11281 atttttgcta ttagattttc tgcaatcgat aatgaaaatg tacgttcctg cgagctacct
    11341 tctaaattga acgcaatgta agatcctaac gatcgcattt cctcgcgcac ctcatcgagt
    11401 acatcctcac ttagtggcaa ttcatatatt ggcctgactg ctggaaaacc cgcctcacgc
    11461 atcataaatg cccatgtcat aggtacggga gcccggagtt tctgatccat acgggacgcg
    11521 ttcttgcaca aaggggagaa gcaattcatg gttataccaa caacctgaaa attcgttttt
    11581 gctttcaact gactgataaa taacatcgtt ttcaggttct ttttacgcat cccctcaatg
    11641 caaagatccg gcgtaccgta ttgctgtgtt atgttctttg ctaaatcttt tatttctttt
    11701 aatgttgcgt gatcctgcat agtcattgtg actaatgtta atttagtctg ttcaagttta
    11761 agcgcattaa agacttctaa attaattgtc gacgttacaa ttaaaagatg cttaatttta
    11821 tgcaattcaa gcgcccgaat aacaggaaag atggccatag catcgccaat ctgatcggga
    11881 atatggatga caacaaagtc tgttttttca atattgaaat tataagcttt ataatcgtag
    11941 taactaaatg caatacgtct caacaatgat actaaaaaca tacctaacct cgcctcccta
    12001 ctggttataa tgcaatgcag tctatcagac tcatcagggt gccattttgt gcatatgcgg
    12061 acttttatgt ttcatatctc taacctgtgg gtcctctgct taatccttaa acaacaccag
    12121 caactcctgc gctttcatct tccatcgaat ttttcatgtt gccgctaatc agccataaaa
    12181 tcatttgcag atgcgctctg tcgaggtagt ctcataaggt tcgtttatag atcgacggca
    12241 atgtgagtta ccttttccat actaattata aaaagacagt acaaacagga tcattatgga
    12301 ctccacgctc atctccactc gtcccgatga agggacgctt tcgttaagtc gcgcccgacg
    12361 agctgcgtta ggcagcttcg ctggtgccgt cgtcgactgg tatgattttt tactctatgg
    12421 catcaccgcc gcactggtgt ttaatcgcga gtttttcccg caagtaagcc cggcgatggg
    12481 aacgctcgcc gcatttgcta cctttggcgt cggatttctt ttccgtccgc tcggcggtgt
    12541 cattttcggt cactttggcg accgactggg acgtaagcgc atgttaatgc tgaccgtctg
    12601 gatgatgggc atcgcgacag ccttgattgg tattcttcct tcattctcga ccattgggtg
    12661 gtgggcacct attttgctgg tgacactgcg tgccattcag ggatttgcag tcggcggcga
    12721 atggggaggc gcggcgttgc tttccgttga aagtgcaccg aaaaataaaa aagcctttta
    12781 cagtagcggt gtacaagttg gctacggtgt aggtttactg ctttcaaccg gactggtttc
    12841 attgatcagt atgatgacga ctgacgaaca gtttttaagc tggggctggc gcattccttt
    12901 cctgtttagc atcgtactgg tactgggagc attgtgggtg cgcaatggca tggaggagtc
    12961 cgcggaattt gaacaacagc aacattatca agctgccgcg aaaaaacgca tcccggttat
    13021 cgaagcgctg ttacgacatc ccggtgcttt cctgaagatt attgcgctac gactgtgcga
    13081 attgctgacg atgtacatcg ttactgcctt tgcacttaat tattcaaccc agaatatggg
    13141 gctaccgcgc gaacttttcc ttaatattgg tttgctggta ggtggattaa gctgcctgac
    13201 aattccctgt tttgcctggc ttgccgatcg ttttggtcgc cgcagggttt atatcacagg
    13261 tacgttaatc ggaacgttga gcgcatttcc tttctttatg gcgcttgaag cacaatctat
    13321 tttctggata gttttcttct ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg
    13381 tgtgcaacaa ccgatgttta ccgaaatgtt tggtgccagt tatcgctata gtggcgctgg
    13441 agtcggttat caggttgcca gtgtggttgg cggtggattt acacctttta ttgccgctgc
    13501 actcatcact tactttgccg ggaactggca tagcgtcgcc atttatttgc tggctggatg
    13561 cctgatttcc gcaatgaccg ctttgttgat gaaagacagt caacgcgctt gatagcctgg
    13621 cgaagatcat ccgatcttcg ccttacactt ttgtttcaca tttctgtgac atactatcgg
    13681 atgtgcggta attgtatgga acaggagaca cacatgaata ataagggctc cggtctgacc
    13741 ccagctcagg cactggataa actcgacgcg ctgtatgagc aatctgtagt cgcattacgc
    13801 aacgccattg gcaactatat tacaagtggc gaattacctg atgaaaacgc ccgcaaacaa
    13861 ggtctttttg tctatccatc actgaccgta acctgggacg gtagcacaac caatcccccc
    13921 aaaacgcgcg catttggtcg ctttacccac gcaggcagct acaccaccac gattactcgc
    13981 cctactctct ttcgttcgta tcttaatgaa caacttacgt tgctgtatca ggattatggt
    14041 gcgcatatct cagtgcaacc ctcgcagcat gaaatccctt atccttatgt catcgatggc
    14101 tctgaattga cacttgatcg ctcaatgagc gctgggttaa ctcgctactt cccgacaaca
    14161 gaactggcgc aaattggcga tgaaactgca gacggcattt atcatccaac tgaattctcc
    14221 ccgctatcgc attttgatgc gcgccgcgtc gatttttccc tcgcacggtt gcgccattat
    14281 accggtacgc cagttgaaca ttttcagccg ttcgtcttgt ttaccaacta cacacgttat
    14341 gtggatgaat tcgttcgttg gggatgcagc cagatcctcg atcctgatag tccctacatt
    14401 gccctttctt gtgctggcgg gaactggatc accgccgaaa ccgaagcgcc agaagaagcc
    14461 atttccgacc ttgcatggaa aaaacatcag atgccagcat ggcatttaat taccgccgat
    14521 ggtcagggta ttactctggt gaatattggc gtgggaccgt caaatgctaa aaccatctgc
    14581 gatcatctgg cagtgctacg cccggatgtc tggttgatga ttggtcactg tggcggatta
    14641 cgtgaaagtc aggccattgg cgattatgta cttgcacacg cttatttacg cgatgaccac
    14701 gttcttgatg cggttctgcc gcccgatatt cctattccga gcattgctga agtgcaacgt
    14761 gcgctttatg acgccaccaa gctggtgagc ggcaggcccg gtgaggaagt caaacagcgg
    14821 ctacgtactg gtactgtggt aaccacagat gacaggaact gggaattacg ttactcagct
    14881 tctgcacttc gttttaactt aagccgggcc gtagcaattg atatggaaag tgcaaccatt
    14941 gccgcgcaag gatatcgttt ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa
    15001 ccgttgcatg gcgagattaa acttcccggc caggctaacc gtttttatga aggcgctatt
    15061 tccgaacatc tgcaaattgg cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga
    15121 ctgcattcgc gtaaattacg aacctttaat gagccgccgt tccgataata agttctgaac
    15181 cttcttatca gaaatagatg tgaaggagta agtaagacac ctggcaaata gcctgcaata
    15241 atcgtgggct gtttgcttcc ttgggcggat acgagtttta ttatcgtctt aatgatttcc
    15301 acatattaaa agcaagtatg ctttcaaaac aca
//
LOCUS       DAPHDX010000102        15239 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPHDX010000102 DAPHDX010000000
VERSION     DAPHDX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.103"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            224..559
                     /locus_tag="RH058_003253"
     CDS             224..559
                     /locus_tag="RH058_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV3138801.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            719..1162
                     /gene="yjdN"
                     /locus_tag="RH058_003254"
     CDS             719..1162
                     /gene="yjdN"
                     /locus_tag="RH058_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV3138802.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1295..2083
                     /gene="phnC"
                     /locus_tag="RH058_003255"
     CDS             1295..2083
                     /gene="phnC"
                     /locus_tag="RH058_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV3138803.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2108..3124
                     /gene="phnD"
                     /locus_tag="RH058_003256"
     CDS             2108..3124
                     /gene="phnD"
                     /locus_tag="RH058_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3138804.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3230..4009
                     /gene="phnE"
                     /locus_tag="RH058_003257"
     CDS             3230..4009
                     /gene="phnE"
                     /locus_tag="RH058_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV3138805.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4030..4755
                     /gene="phnF"
                     /locus_tag="RH058_003258"
     CDS             4030..4755
                     /gene="phnF"
                     /locus_tag="RH058_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV3138806.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4756..5208
                     /gene="phnG"
                     /locus_tag="RH058_003259"
     CDS             4756..5208
                     /gene="phnG"
                     /locus_tag="RH058_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV3138807.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5205..5789
                     /gene="phnH"
                     /locus_tag="RH058_003260"
     CDS             5205..5789
                     /gene="phnH"
                     /locus_tag="RH058_003260"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV3138808.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILYE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5789..6853
                     /gene="phnI"
                     /locus_tag="RH058_003261"
     CDS             5789..6853
                     /gene="phnI"
                     /locus_tag="RH058_003261"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV3138809.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTAHSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPGERPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6846..7691
                     /gene="phnJ"
                     /locus_tag="RH058_003262"
     CDS             6846..7691
                     /gene="phnJ"
                     /locus_tag="RH058_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV3138810.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7688..8446
                     /gene="phnK"
                     /locus_tag="RH058_003263"
     CDS             7688..8446
                     /gene="phnK"
                     /locus_tag="RH058_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV3138811.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8557..9237
                     /gene="phnL"
                     /locus_tag="RH058_003264"
     CDS             8557..9237
                     /gene="phnL"
                     /locus_tag="RH058_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV3138812.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9234..10370
                     /gene="phnM"
                     /locus_tag="RH058_003265"
     CDS             9234..10370
                     /gene="phnM"
                     /locus_tag="RH058_003265"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV3138813.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10370..10927
                     /gene="phnN"
                     /locus_tag="RH058_003266"
     CDS             10370..10927
                     /gene="phnN"
                     /locus_tag="RH058_003266"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV3138814.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10914..11348
                     /gene="phnO"
                     /locus_tag="RH058_003267"
     CDS             10914..11348
                     /gene="phnO"
                     /locus_tag="RH058_003267"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV3138815.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            11350..12108
                     /gene="phnP"
                     /locus_tag="RH058_003268"
     CDS             11350..12108
                     /gene="phnP"
                     /locus_tag="RH058_003268"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV3138816.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHYDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12255..12584)
                     /gene="yjdP"
                     /locus_tag="RH058_003269"
     CDS             complement(12255..12584)
                     /gene="yjdP"
                     /locus_tag="RH058_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDV3138817.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRCRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12653..12970)
                     /locus_tag="RH058_003270"
     CDS             complement(12653..12970)
                     /locus_tag="RH058_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDV3138818.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13223..15208
                     /gene="yjcS"
                     /locus_tag="RH058_003271"
     CDS             13223..15208
                     /gene="yjcS"
                     /locus_tag="RH058_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV3138819.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3452 a         4511 c         4189 g         3087 t
ORIGIN      
        1 gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa atagaggcaa
       61 aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc
      121 agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg gcaatctgcc
      181 gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat taccacactg
      241 cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct gcccggaatg
      301 tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga tcgttaaaga
      361 tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag atctgaaggt
      421 gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc gcctggttga
      481 aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac tgaaatctga
      541 gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc
      601 atccggcaac gatgccgact gcctgatgcg acgctgaccg cgtcttatca ggcttccctc
      661 tcatctgtat aaatttcgaa ctacacttaa ctggcttctc ttaactgagg tcaccatcat
      721 gccgttaagt ccctacctct cttttgccgg taactgtgcc gacgcaattg cttattatca
      781 acgtacgttg ggcgctgaac tgctctataa aatcagcttc ggcgaaatgc caaaatcagc
      841 gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg ccatcgctca
      901 tgccaacgtg cgcattgccg gaagcgacat catgatgagc gatgccatgc cgtcaggaaa
      961 agccagctac tccggcttta cgctggtgct cgattcgcaa caggtcgaag aaggaaaacg
     1021 ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg gcctggcagg aaactttctg
     1081 ggcgcatggc tttggcaaag tcaccgataa atttggcgta ccgtggatga ttaatgtcgt
     1141 caaacaacaa ccaacgcaat aatccgccgg gaggcccgcc ctcccgcact gtcatcgaat
     1201 tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac aataatccgc
     1261 cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc gtgtcgagaa
     1321 gctcgccaaa accttcaatc agcatcaggc gctgcatgcg gttgatctga acattcatca
     1381 cggtgaaatg gtggctctgc ttgggccgtc gggttccggc aaatccaccc ttttacgtca
     1441 cttaagcggt ttgattaccg gcgataaatc cgccggcagc catatcgagc tgctgggccg
     1501 cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc aaaagccgcg ccaacaccgg
     1561 ctacatcttc caacaattca acctggtgaa ccgcctgagc gtactggaga acgtgctgat
     1621 tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt agctggttca ccggcgagca
     1681 gaaacagcgc gcgttacagg cgctgacccg cgttggcatg gtgcattttg cccatcagcg
     1741 cgtttccacc ctctccggcg gacagcagca gcgtgtggcg attgcccgcg cgctgatgca
     1801 gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg ctggacccgg aatccgcccg
     1861 catcgtgatg gacaccctgc gcgacatcaa tcagaacgac ggcatcaccg tggtcgtcac
     1921 gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc tgcgccaggg
     1981 gcacgttttc tacgacggca gcagccaaca gtttgataac gaacgttttg accatctcta
     2041 ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc tgacatcccc atcattgagg
     2101 aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt tcagcctcag
     2161 caccctgtta agcccggcac acgccgaaga gcaggaaaag gcgctgaatt tcggcattat
     2221 ttcaacggaa tcacagcaaa accttaaacc gcaatggacg ccattcttac aggatatgga
     2281 gaagaagctg ggcgtgaagg tgaacgcctt ctttgcccca gactacgcag gcattatcca
     2341 gggaatgcgc ttcaataaag tggatatcgc ctggtacggc aacctgtcgg caatggaagc
     2401 ggtggatcgc gccaacggcc aggtcttcgc ccagacggtc gcggcggatg gatcgccagg
     2461 ttactggagc gtgttgatcg tcaacaaaga tagtccgatc aacaacctga acgatctgct
     2521 ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct aactccacct ctggcttcct
     2581 cgtccccggt tactacgtct ttgccaaaaa caatatctcc gccagcgact ttaagcgcac
     2641 cgtcaacgct gggcatgaaa ccaacgcgct ggccgtcgcc aacaagcagg tggatgttgc
     2701 caccaacaac accgaaaacc tcgacaagct gaaaacctcc gcgccagaga agctgaaaga
     2761 actgaaggtg atctggaagt cgccgctgat cccaggcgat ccgatcgtct ggcgtaaaaa
     2821 cctttccgaa accaccaaag acaagatcta cgacttcttt atgaactacg gcaaaacgcc
     2881 ggaagaaaaa gcggtgctgg aacgcctggg ctgggcgccg ttccgcgctt ccagcgacct
     2941 gcaactggtg ccgattcgcc agctcgcgct gtttaaagag atgcagggcg tgaaaagcaa
     3001 taaaggactg aatgagcagg acaagctggc aaaaaccacc gagattcagg cgcagctgga
     3061 tgacctggac cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta aagcggtgca
     3121 gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc gacaatgtgc aggcgttgat
     3181 gccggatgcg gtgcaagcac cttatccggc ctacagaccg gagccaaaca tgcaaaccat
     3241 caccatcgcc ccacccaagc gtagctggtt ctcgcttctg agctgggccg ttgttctcgc
     3301 cgtgttggtc gtctcgtggc agggcgcgga aatggccccg ctcacgctga ttaaagacgg
     3361 cggcaacatg gcgacgttcg ccgccgactt cttcccgccc gatttcagcc agtggcagga
     3421 ttacctcacc gaaatggccg tcacgctgca aatcgccgtc tggggcacgg cgctggcggt
     3481 ggttctctcc atccccttcg gcctgatgag cgccgaaaac ctggtgccgt ggtgggttta
     3541 ccagcccgtt cgccgcctga tggacgcctg ccgcgccatt aacgaaatgg tcttcgccat
     3601 gctgttcgtg gtcgccgtcg gcctcggccc gttcgctggc gtgctggcgc tgtttatcca
     3661 caccaccggc gtgctctcca aactgctttc cgaagcggtg gaagcgattg aacccggccc
     3721 ggtggaaggc attcgcgcca ccggcgccaa caagctcgaa gagatcctct acggcgtgct
     3781 gccgcaggtg atgccgctgc tgatctccta ctccctctat cgcttcgaat ccaacgtccg
     3841 ctcggcgacc gtcgtcggca tggtcggtgc aggcgggatc ggcgtcaccc tgtgggaagc
     3901 gattcgcggt ttccagttcc aacaaacctg cgccctgatg gtgcttatca tcgtcacggt
     3961 cagcctgctg gatttcctct ctcaacggtt gcgtaagcac tttatctgat aagcgaggca
     4021 ttgatatcta tgcacttgtc tacacatccg accagctacc caacacgcta tcaggagata
     4081 gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct gcggcgacta tcttcccgcc
     4141 gagcagcaac tggcggcgcg ctttgaggtg aaccgccaca ccctgcgccg cgccatcgac
     4201 caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg tcggcgtgct ggtgctgatg
     4261 cgcccgttcg attacccgct caacgcccag gcgcgtttta gccagaacct gctggaccag
     4321 ggtagccatc ccaccagcga aaaactgctg tcggtattgc gccccgcgtc cggtcacgtc
     4381 gctgacgcgc tggggattac cgagggcgag aacgttatcc atttgcgcac cctgcgtcgc
     4441 gtcaacggcg tcgcgctctg tttaatcgac cactactttg cggacctcac cctttggccg
     4501 acgctgcaac gcttcgacag cggttcgctg cacgattttc tgcgcgagca aaccggaatt
     4561 gcgctgcgcc gcagccagac gcggatcagc gcccgtcgcg cccaggccaa agagtgccag
     4621 cgtcttgaaa ttccgaatat gtcgccgctg ctgtgcgtgc gcacccttaa ccaccgtgac
     4681 ggtgaaagca gcccggcgga gtactccgtc agcctgacgc gcgccgacat gattgaattc
     4741 actatggagc actgaatgca cgcagatacc gcgacccgcc agcactggat gtccgtactg
     4801 gcgcacagcc aaccggctga actggcagcc cgcctgaaag cactgaacat caccgccgac
     4861 tatgaggtta tccgcgccgc cgaaaccggc ctggtgcaga ttcaggcgcg gatgggcggc
     4921 accggcgaac gcttttttgc cggcgacgcc acgctcaccc gcgccgccgt acgcctgact
     4981 gacggcacgc tcggctacag ctgggtgctg gggcgcgaca aacagcacgc cgaacgctgc
     5041 gcgctgattg acgcgctgat gcagcaatct cgctattttc aaaacttatc agaaaccctt
     5101 attgccccgc tggacgctga ccgtatggca cgcattgccg cacgccaggc cgaagtgaac
     5161 gccagccggg tcgacttctt tacgatggtt cgcggagata acgcatgacc ctggaaaccg
     5221 cttttattct tcctgtgcag gatgcccagc acagttttcg tcgcctgtta aaggccatga
     5281 gcgagccggg cgtgattgtc gccctgcatc agctcaaacg cggctggcag ccgctgaata
     5341 tcgccaccac cagcgtgctg ctgacgctgg ccgataacga cacgccggtg tggctttccg
     5401 cgccattgag taacgacatc gtcagccaga gcctgcgttt tcataccaac gcgccgctgg
     5461 tcagccagcc ggaacaggcg accttcgcgg taacggatga ggcgatttcc agcgaacagc
     5521 ttaacgccct gtccaccggc accgccgttg cgccggaagc gggtgcaacg ctgattttac
     5581 aggtcgccag cctgagcggc ggacgcatgt tacgtctcac cggcgcgggc attgccgaag
     5641 aacgaatgat cgccccgcag ctgccggagt gcattctgta cgaactcacc gagcgcccgc
     5701 acccgttccc gctcggcatc gacctgatcc tgacctgcgg cgagcgcctg ctggctattc
     5761 cgcgaaccac gcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg gcgaaaaggc
     5821 gatcgatgcc gcccacgccc tgcaagagag ccgacgccgg ggcgataccg atttgcctga
     5881 actgagcgtc gcccagattg aacagcagct taacctcgca gtagatcgcg tgatgaccga
     5941 aggcggcatt gccgaccgcg aactggcggc gctggcgctg aaacaggcca gcggcgataa
     6001 cgtcgaagca attttcctgc tgcgcgccta ccgcaccacg ctggcgaagc tcgcggtaag
     6061 cgagccgctc gacaccacgg agatgcgtct cgaacggcgt atttcagcgg tttataaaga
     6121 tattcccggc ggccagttgc ttggcccaac ctacgactac acccatcgcc tgctcgattt
     6181 caccctgctg gcaaacggcg aagcgccgac gctgaccacc gcccacagcg aacaacagcc
     6241 gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg gcgaagtttg aagaggatag
     6301 cggcgcacag ccggatgaca tcacccgcac gccgccggtt tacccctgct cacgctcctc
     6361 ccgtttacag cagttgatgc gcggcgacga aggctatttg ctggcgctgg cctactccac
     6421 ccaacgcggc tacggtcgca accacccgtt cgcaggcgag atccgcagcg gctatatcga
     6481 cgtatcgatt gtgccggaag agctgggatt tgcggtgaac gtcggcgaac tgctgatgac
     6541 cgagtgtgaa atggtcaacg gttttatcga cccgccgggt gagcggccgc acttcacgcg
     6601 cggctacggg ctggtgttcg gcatgagcga gcgcaaagcg atggcaatgg cgctggtcga
     6661 tcgtgcgttg caggctccag agtacggcga gcacgcgaca ggcccggcgc aggatgaaga
     6721 gttcgtgctg gcgcatgccg acaatgtcga agccgcaggc tttgtctcgc acctgaaact
     6781 cccgcactac gtcgatttcc aggccgaact ggagctactc aaacgtctgc aacaggagca
     6841 gaaccatggc taatctgagc ggctacaact ttgcgtacct cgacgagcag accaaacgca
     6901 tgatccgccg cgccatctta aaagcggtgg cgatccccgg ctatcaggtg ccgtttggcg
     6961 ggcgcgagat gccgatgcca tacggctggg gaaccggcgg catacagctc accgccagcg
     7021 tgattggcga aagcgacgtg ctgaaggtga ttgaccaggg cgcagacgac accaccaacg
     7081 ccgtgtcgat ccgcaacttc tttaagcgcg tgaccggggt aaacaccact gaacggacgg
     7141 acgatgcgac gcttatccag acgcgccatc gcatccccga aacgccgctc accgaagatc
     7201 agatagttat tttccaggtg ccaatccccg agccgctgcg ctttatcgag ccgcgcgaaa
     7261 cggaaacccg caccatgcac gcgctggagg agtacggcgt gatgcaggtg aaactgtatg
     7321 aagatattgc ccgcttcggt catatcgcca ctacctacgc ctatccggtg aaggttaacg
     7381 ggcgctacgt aatggacccg tcgccgatcc cgaaattcga taacccgaaa atggacatga
     7441 tgcccgccct gcaactgttc ggcgcggggc gcgagaagcg catctatgcg gtgccgccgt
     7501 ttacccgcgt ggaaagtctc gatttcgacg atcacccgtt caccgttcag cagtgggatg
     7561 agccatgcgc catctgcgga tcgacccaca gctatcttga tgaagtggtg ctggatgacg
     7621 ccggaaaccg catgtttgtc tgctccgata ccgattattg ccgccaacag agcgaggcga
     7681 aaagccaatg aataaaccgt tactttcggt caataacctg acccaccttt acgcgccggg
     7741 caaaggcttt agcgatgttt cttttgattt atggccggga gaagtgctgg gcattgtcgg
     7801 agaatccggc tccgggaaga ccacgctgct gaagtcgatc tccgcgcgtc tgacgccgca
     7861 gcagggggag attcactacg agaaccgttc gctgtatgca atgagcgagg ccgaccgccg
     7921 tcgcctgctg cgcaccgaat ggggcgtagt gcatcagcat ccactcgacg gcctgcgccg
     7981 tcaggtctcg gcaggcggca atatcggcga gcggctgatg gcgaccgggg cgcgtcatta
     8041 cggcgacatt cgcgccaccg cgcagaagtg gctggaagag gtggaaattc ccgccaaccg
     8101 gatcgacgac ctgccgacca ccttttccgg cggcatgcag caacgtttgc agattgcccg
     8161 caacctggtg acgcatccga agctggtgtt tatggatgaa ccgaccggcg ggctggatgt
     8221 gtcggtgcag gcccgcctgc tcgacctgct gcgcggcctg gtggtggagc tgaacctggc
     8281 ggtggtgatt gtcacccatg atttaggcgt tgcccgcctg ctggcggacc gtttgctggt
     8341 gatgaagcag gggcaagtgg tggagagtgg gttaaccgac cgcgtgctcg acgacccgca
     8401 tcatccgtat acacagctgc tggtgtcatc ggttttgcag aattgagccg gtgccggatg
     8461 cggcgtaaac gccttatccg gcctacaaat gcgctccccg taggtcggat aagacgcgtc
     8521 agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga ttaacgtaca aaacgtcagt
     8581 aaaaccttca tcctgcacca gcaaaacggc gtgcgcctgc ccgtcctcaa ccgcgcctcg
     8641 ctcaccgtca acgcgggcga atgcgtggtg ctccacggtc actccggcag cggcaaatca
     8701 accctgctgc gctcgctgta cgccaactat ctgcccgatg aaggtcaaat ccagatcaaa
     8761 cacggtgacg agtgggtaga cctggtcacc gcgccagccc gcaaagtggt ggagatccgc
     8821 aaaaccaccg tcggctgggt gagccagttt ctgcgcgtca tcccgcgtat ttcagcactg
     8881 gaagtggtga tgcagccgct gctcgatacc ggcgttccgc gtgaagcctg cgccgctaaa
     8941 gccgcgcgtc tgctcacccg cctgaacgtg ccggaacgcc tgtggcacct ggcgccatca
     9001 accttttccg gtggcgaaca gcagcgcgtc aacatcgccc gcggctttat cgtcgactac
     9061 cccattctgc tgctcgacga acctaccgcc tcgctggacg ccaaaaacag cgccgccgta
     9121 gtcgagctga ttcgcgaagc caaaacccgt ggcgcggcca tcgtcggcat cttccatgac
     9181 gaagccgtac gtaatgacgt cgccgaccgc ttgcacccaa tgggagcctc tgcatgatta
     9241 tcaataacgt caaactggtg ctggaaaacg aagtggtaca cggttcgctg gaggtgcagg
     9301 atggcgaaat ccgcgccttt gccgaaagcc agagccgcct gccagaagcg atggacggcg
     9361 aaggtggctg gctactaccc ggcctgattg agctgcatac cgacaacctc gataaattct
     9421 tcaccccgcg cccgaaagtc gactggcccg cccattcggc gatgagcagc cacgacgcgc
     9481 tgatggtggc aagcggcatc accaccgtgc tggacgcggt ggcgattggc gacgtgcgcg
     9541 acggcggcga tcggctggag aatctggaga agatgatcaa caccatcgaa gagacgcaga
     9601 aacgcggcgt caaccgcgcc gagcaccgcc tgcacctgcg ctgcgaactg ccgcatcaca
     9661 ccacactgcc gctgtttgaa aaactggttc agcgcgaacc ggtgacgctg gtgtcgctga
     9721 tggaccactc accgggccag cgccagttcg ctaaccgcga gaagtatcgc gaatattatc
     9781 agggcaaata ctccctcacc gatgcgcaga tgcagcagta cgaagaagag caattggcgc
     9841 tcgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc cgccctgtgc cgcgcgcgac
     9901 aaattgcgct cgccagccac gatgacgcca cacacgccca cgttgccgaa tcccaccaac
     9961 ttggcagcgt gatcgccgaa tttcccacca cgttcgaagc ggcggaagcc tcgcgtaagc
    10021 atggcatgaa cgtgctgatg ggtgcgccaa atatcgtgcg tggcggctcg cactccggca
    10081 atgtggcggc cagtgaactg gcgcagcttg gtctgctgga tatcctctct tccgactact
    10141 accccgccag cctgctggat gcggcgttcc gcgtcgccga tgacgagagc aaccgcttta
    10201 cgctaccgca ggcggtgagg ctggtgacca aaaatccggc gcaggcgctg aatcttcagg
    10261 atcgcggggt gattggcgag ggtaaacgcg ctgacctggt gctggcgcat cgccagggca
    10321 atcacattca tatcgaccac gtctggcgtc agggtaaaag ggtgttctga tgatgggaaa
    10381 actgatttgg ttaatggggc cgtccggctc cgggaaagac agcctgctgg cggaactccg
    10441 cctgcgggaa caaacgcagt tactggtggc gcatcgctac atcacgcgcg acgccagcgc
    10501 cggaagtgaa aaccatatcg ccctgagtga gcgggagttt tttacccgcg cggggcaaaa
    10561 cctgctggcc ttaagctggc acgctaacgg tctgtattat ggcgtcggca tcgagattga
    10621 cctctggctg cacgctggat tcgacgtagt cgtcaacggt tcacgcgcgc atttgccgca
    10681 ggcacgggcg cgctatcaat cggcgctgct gcccgtctgt ttacaggttt cgccggagat
    10741 cctgcgccag cgcctggaaa accgtggccg tgaaaacgcc agtgaaatta acgcccgcct
    10801 ggcgcgcgcc gcccgctata ctccacagga ttgccatacg ctcaacaatg acggcagcct
    10861 gcgccagtcg gtcgacacgc tgctgacgct gatccatcag aaggagaaac accatgcctg
    10921 cttgtgagct tcgcccggcc acgcagtacg ataccgacgc ggtttacgcg ctgatttgtg
    10981 agctaaaaca ggcggagttt gaccaccatg cgtttcgcgt gggctttaac gccaacctgc
    11041 gcgacccaaa catgcgctac catctggcgc tgctggatgg cgaagttgtc ggcatgatcg
    11101 gcctgcattt gcagtttcat ctgcatcatg tcaactggat cggcgaaatt caagaactgg
    11161 tggtgatgcc gcaggcgcgc ggtctgaacg tcggcagtaa attactggca tgggcagaag
    11221 aagaagcccg ccaggccggg gccgaaatga ccgagctttc caccaacgtg aagcgccaca
    11281 acgcacaccg tttttacctg cgtgaaggct acgagcagag ccacttccgc ttcaccaagg
    11341 cgctgtaaca tgagcctgac cctcacgctc accggcactg gcggcgcaca gggcgttccg
    11401 gcatggggct gcgagtgcgc ggcctgcgcc agagcgcggc gctcgccgca gtatcgccgc
    11461 cagccatgca gcggcgtagt gaagtttaac gacgcgatca ccctgatcga cgccgggcgg
    11521 cacgatctca ccgatcgctg gtcgcctgga tcgttccagc agtttttgct gacgcactac
    11581 catatggatc acgtccaggg gctatttccg ctacgctggg gcgtgggcga tgtgatcccg
    11641 gtctacggcc cgccggatga acagggctgc gacgatctgt ttaaacatcc gggcctgctt
    11701 gatttcagcc acacagtgga accgtttgtg gtgtttgatt tgcaggggtt gcaggtcacg
    11761 ccgctgccgc ttaaccactc aaaactgacc ttcggttatc tgctggaaac ggcacatagc
    11821 cgggtggcgt ggctgtctga caccgctggc ctgccggaaa aaacgctgaa atttttactc
    11881 aataatcatc cgcaggtgat ggtgattgat tgcagtcacc cgccgcgcgc ggatgcaccg
    11941 cgtaatcact atgatttaaa taccgtgctt gcgctgaatc aggttatccg ctcgccacag
    12001 gtgattctga cccatatcag ccaccagttt gatgcgtggc tgatggaaaa cgcactaccg
    12061 tcagggtttg aggtggggtt tgatgggatg gagattgggg tggcgtgatg agagggaatg
    12121 tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg gcgaggggac cgattgtgct
    12181 cgatattgaa tattgcgctc gttttctccc tctccccatt ggggtgaggg gcgatgcctg
    12241 ctccataccc aacctcatcg cccatactca tcttccattc tccgctcttc atcctccagt
    12301 tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca gctgcctgcg gcgatcttca
    12361 aactggcggc ggcggtcgtc atattgtctg caccgatcgt cgctcacttc acgctgccag
    12421 ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag ggttataagc gtcattaatc
    12481 gcctgctgaa tattaccaat agtatcgtcg ataatatcag cctgggccgg aacggtggac
    12541 agcgtgagca gggtgaataa aagaaatagc ggaaagcgtt tcattagcca acctcaaaaa
    12601 gaaactctat ccacattaat cattactcat ccatgcaagt agtggatgaa tctcaatttc
    12661 tccgctgctc tattgccgta atcgcctcca cgcgttgttg atgacgaccg ccttcgtact
    12721 gtgcgcccag ccacgcatcc acaatcattt ttgccagttc gaggccaacc actcgtgaac
    12781 caaaagccag tgcaacttca ctggcataat gcggataatc agtgcgctct gacgacctgg
    12841 tgtctttatc aatcacctca ctcattgacc atgaataaaa ggctggcaca gcaacgcaga
    12901 atcctgcggt gccagcgaca tgaccgccca ttgctccggt acgtttttta aaaagaaaca
    12961 ccggttgcat tgataaattg tgctcagtat ttgccgaagt acaatatata taataaatat
    13021 ataaaacacc tcatattatt tacagagata ataaattcgc gtaattttcc ctcttcagca
    13081 caaatgccta ataaaatggc tcgttccgct cacacttcca cttattaaag tcgcaatttc
    13141 ccaattatga taacgccagc tttaaatgac gtattttgca tggattacat atttacgaca
    13201 caaatgataa atggagattt atatgaataa ctctcggtta ttccgtttga gcaggattgt
    13261 tattgcgtta actgctgcca gcggcatgat ggtaaatacc gcttacgcaa cagatgaagc
    13321 gaaagccgcc actcaatata cccaacaggt taatcagaat tacgccaaat cattaccgtt
    13381 tagcgatcgt caggattttg acgacgctca gcgtggattt atcgctccgc tgctggatga
    13441 aggtattctg cgcgatgcta atggcaaacc atattatcgc ggggaagatt ataaatttga
    13501 tatcaatgcg cccgcgcccg aaaccgttaa ccctagcctg tggcgtcagt cgcagttaaa
    13561 cggtatttcc ggcctgttta aagtcaccga cagaatgtac caggttcgcg gtcaggatat
    13621 ctccaacatc acctttatcg aaggtgatac cgggattatt gtcatcgacc cgctggtgac
    13681 gccaccgagc gcaaaagccg cccttgacct ttacttccaa aatcgcccgc aaaaaccgat
    13741 tgttgcggtt atttataccc acagccatac cgaccactat ggcggcgtga aaggcattat
    13801 ctctgaagcc gatgtgaaat ccggtaaagt gcaggttatc gcccctgctg gctttatgga
    13861 cgaagccatc agcgaaaacg tactggcggg taatattatg agccgccgtg cactttactc
    13921 ctacggcctg ctgctggcgc ataaccctca gggtaacatc ggcaatggtc ttggcgtaac
    13981 gctggcatcg ggctacccga gcatcatcgc accgaacaaa accatcacca aaaccggtga
    14041 gaagatgatt atcgacggcc tggagtttga cttcctgatg accccaggta gcgaagcacc
    14101 agccgaaatg cacttctata ttccggccct gaaagcgctg tgtaccgccg agaacgccac
    14161 gcataccctg cacaacttct acactctgcg cggcgcgaaa acccgcgaca ccagcaagtg
    14221 gaccgagtat ctgaacgaaa cgctggatat gtggggtaac gacgcggaag tcctgtttat
    14281 gccgcacacc tggccggtct ggggcaataa gcatatcaat gattatattg gtaaatatcg
    14341 cgatactatc aagtacattc acgaccagac cctgcacctg gcgaaccagg gctacaccat
    14401 gaatgaaatc ggcgacatga ttaaactgcc gcctgcactt gccaataact gggccagccg
    14461 tggctattac ggttctgtca gccacaacgc ccgcgcggtg tataacttct atcttggcta
    14521 ttacgacggt aacccggcta acctgcatcc gtatggtcag gtggagatgg gtaaacgtta
    14581 cgtgcaggcg ctgggcggtt ctgcccgtgt catcaacctg gcgcaagaag cgaacaagca
    14641 aggtgattac cgctggtcgg cagaactgct gaaacaggtg attgccgcca acccgggtga
    14701 ccaggtcgcg aagaatctgc aagcgaataa ctttgaacag ctgggctatc aggccgagtc
    14761 cgccacctgg cgcggtttct acctgaccgg cgcgaaagag ctgcgcgaag gggtgcataa
    14821 gttcagccac ggcaccaccg gttccccgga caccattcgc gggatgtcgg tcgaaatgct
    14881 gttcgacttt atgtccgttc gcctcgatag cgcgaaagcc gcgggtaaaa atatcagcct
    14941 gaacttcaat atgagcaatg gcgataacct caacctgacg ctgaacgata gcgtgcttaa
    15001 ctaccgtaaa acactgcaat cccaagctga cgcctctttc tacatcagcc gtgaagatct
    15061 gcacgccgtg ctgaccgggc aagccaaaat ggcggatctg gtaaaagcga agaaagccaa
    15121 aattattggc aatggcgcga aactggaaga aattatcgcc tgtctggata atttcgattt
    15181 gtgggtgaat atcgtaaccc caaattaatg tcactcgctt ttgggggcgc tgaagcccc
//
LOCUS       DAPHDX010000103        15030 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPHDX010000103 DAPHDX010000000
VERSION     DAPHDX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.104"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            133..375
                     /locus_tag="RH058_003272"
     CDS             133..375
                     /locus_tag="RH058_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138820.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            489..1127
                     /locus_tag="RH058_003273"
     CDS             489..1127
                     /locus_tag="RH058_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV3138821.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1124..1861
                     /locus_tag="RH058_003274"
     CDS             1124..1861
                     /locus_tag="RH058_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV3138822.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1861..3957
                     /locus_tag="RH058_003275"
     CDS             1861..3957
                     /locus_tag="RH058_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV3138823.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4004..4282)
                     /locus_tag="RH058_003276"
     CDS             complement(4004..4282)
                     /locus_tag="RH058_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138824.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4551..4961
                     /gene="psiE"
                     /locus_tag="RH058_003277"
     CDS             4551..4961
                     /gene="psiE"
                     /locus_tag="RH058_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV3138825.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5005..6480)
                     /gene="xylE"
                     /locus_tag="RH058_003278"
     CDS             complement(5005..6480)
                     /gene="xylE"
                     /locus_tag="RH058_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV3138826.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6852..7742)
                     /gene="malG"
                     /locus_tag="RH058_003279"
     CDS             complement(6852..7742)
                     /gene="malG"
                     /locus_tag="RH058_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV3138827.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7757..9301)
                     /gene="malF"
                     /locus_tag="RH058_003280"
     CDS             complement(7757..9301)
                     /gene="malF"
                     /locus_tag="RH058_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV3138828.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9455..10645)
                     /gene="malE"
                     /locus_tag="RH058_003281"
     CDS             complement(9455..10645)
                     /gene="malE"
                     /locus_tag="RH058_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV3138829.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11010..12125
                     /gene="malK"
                     /locus_tag="RH058_003282"
     CDS             11010..12125
                     /gene="malK"
                     /locus_tag="RH058_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV3138830.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12197..13537
                     /gene="lamB"
                     /locus_tag="RH058_003283"
     CDS             12197..13537
                     /gene="lamB"
                     /locus_tag="RH058_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV3138831.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13780..14700
                     /gene="malM"
                     /locus_tag="RH058_003284"
     CDS             13780..14700
                     /gene="malM"
                     /locus_tag="RH058_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV3138832.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3714 a         3871 c         3955 g         3490 t
ORIGIN      
        1 ttcattccaa ttatcctaaa accccatctc taattccccg cgccgtaatt cgcatgcttt
       61 agttgtgtat actcgatccc gcccgaaatg tttttgggta aatctccatt cattcaatga
      121 agggaaattg ttatgaaaaa agttctgtat ggcatttttg ccatatctgc gcttgcggcg
      181 acttctgcgt gggctgcacc tgtacaggtg ggcgaagcgg cagggtcggc agcaacgtcg
      241 gtttcggcgg ggagttcctc cgcgaccagc gtcagcaccg taagctcggc ggtgggtgtc
      301 gcgctcgcgg caaccggtgg cggtgatggt tctaataccg ggaccaccac aaccacgacc
      361 accagtaccc agtaataaag tatgtatccc caaaataatt tgagtcattg catctgtggc
      421 ttgaagtatg aagggattta ccataaccac actccggtgt ggttattctg cccctctgga
      481 gaagagtcgt gaagcgacct gcactcattc ttatctgcct gctattacag gcctgttcag
      541 ccacgactaa agagctgggc aattcactgt gggacagtct gttcggcacg ccaggcgtac
      601 aactgacgga cgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg
      661 gcgggccgca attgtttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca
      721 ctcaggatca agccactctt gtcatacaac atggtcgtct ggtgaagact ttgcttggcg
      781 gcgacaacct gattgaagtg aataaccttg ccgccgaccc gctgattaaa cccgcacaaa
      841 ttgttgatgg tgcaacatgg acgcgcacga tgggctggac cgagtaccag caggtacgct
      901 acgccaccgc acgctcagtc ttcaaatggg atggcaccga taccgtcaag gtcggcagcg
      961 atgaaacccc ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata
     1021 accgctactg gatcgacagc gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg
     1081 attatttccc ggtgaaaacc actctcatca aggcggcaaa acaatgatta aacaaactat
     1141 tgtcgcattg attttgagtg tgggggcgtc atcggtcttc gcggcgggaa ccgtcaaggt
     1201 attcagcaat ggcagcagtg aggccaaaac gctgacgggc gcagagtatt taatcgatct
     1261 ggtaggccag ccgcggctgg caaacagttg gtggcctggt gcggtgatta gcgaagagct
     1321 ggcaacggcg gcagcattgc ggcagcagca ggcgttgctg acacggctgg cagaactggc
     1381 tgcagattcc agcgccgacg atgccgccgc gattaacgcc ttacgccagc aaattcaggc
     1441 gttgaaagtg acgggcaggc aaaaaatcaa tcttgatccc gatatcgtcc gcgttgccga
     1501 acgcggtaac ccaccgttgc agggcaacta cacgctgtgg gtcgggccac caccgtccac
     1561 ggtcacgctg ttcgggctta tcagccgtcc tggcaagcag ccattcactc caggtcgcga
     1621 cgtggcgagc tatctctccg ggcagaacct gcttagcggt gcggatcgca gctacgcgtg
     1681 ggtggtttac ccggacggac gcacgcaaaa agcgccggtg gcttactgga acaagcgtca
     1741 cgtagagccg atgcccggca gcattattta tgttggcctc gcggactccg tctggagtga
     1801 gacgcctgat gcccttaacg ccgacattct tcagactctg acgcagcgga taccccaata
     1861 atgaaaaaaa gacatctgct tagcttactg gcgctgggca ttagcacagc ttgctacggc
     1921 gaaacatatc ctgcgcccat tggtccgtcg cagtcggatt tcggtggcgt aggattatta
     1981 caaactccca ccgcgcgtat ggcacgggaa ggggagttga gtctgaacta tcgcgataac
     2041 gatcagtacc gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg
     2101 cgctacaccg acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa
     2161 acgtataaag ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttactggctg
     2221 ccgcaagtgg cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat
     2281 cttgttgcca gcaaagcctg ggggccgttc gattttacgc tcggcctggg ctggggatat
     2341 ctgggcacca gcggtaatgt aaaaaatccg ctctgttcag ccagtgataa atattgctat
     2401 cgcgataaca gctacaaaca ggcgggatct atcgacggta gccagatgtt ccacggtcct
     2461 gcctcactgt ttggcggcgt ggaataccag acgccctggc aaccgctgcg cctgaaactg
     2521 gagtatgaag gcaataatta tcagcaggat tttgccggga agctggagca aaaaagtaag
     2581 tttaacgtcg gtgcgattta tcgcgttacc gattgggctg acgttaacct tagctatgaa
     2641 cgtggcaaca cctttatgtt tggcgtcacg ctgcgcacca actttaacga tctgcgcccg
     2701 tcttacaacg ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag
     2761 cattcggtgg tggcgaatca gttaacgctg ttgaaataca atgccggact tgccgatccg
     2821 cagatccagg cgaaaggcga tacgctgtat gtcaccggcg agcaggtgaa atatcgtgat
     2881 tcgcgcgaag ggatcatccg tgctaatcgg atcgtgatga acgatctgcc ggatgggatc
     2941 aaaacgatcc gcattacgga aaatcgtctt aacatgccgc aggtgacgac ggaaaccgat
     3001 gtcgccagcc tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gaagctggcg
     3061 caaaaacgcg tcgagccagt ggttccgcag tccaccgagc agggctggta tatcgacaaa
     3121 tcacgctttg atttccacat cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac
     3181 ttttacatgt atcagctggg cgtgatggga acggcagatt tgtggctgac ggaccatctg
     3241 ctgaccaccg gcagcctgtt tgcaaatctt gccaacaact acgacaagtt taactacacc
     3301 aatccgccgc aggactcgca cttaccgcgc gttcgtaccc atgtgcgcga gtatgtgcag
     3361 aacgatgtct atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc
     3421 tacggtcagg tctatggagg ttatctcgaa accatgtttg gcggtgcggg ggccgaagtg
     3481 ttgtatcgtc cgctggacag taactgggcg tttggtctgg atgccaacta cgttaaacag
     3541 cgcgactggc gtagtgcaaa agatatgatg aaattcaccg actacagcgt gaaaaccgga
     3601 catctgaccg cctactggac gccatctttc gcccaggacg tgttggtgaa agccagcgtc
     3661 gggcagtatc tggcagggga taaaggcggc acgctggaga tcgccaaacg ctttgatagc
     3721 ggcgtggtgg ttggcggcta tgccacgatc accaacgttt cgaaagagga gtacggcgaa
     3781 ggggacttca ccaaaggcgt gtatgtctcg gtaccgttgg atctcttctc gtctggcccg
     3841 acacgcagcc gtgcggcgat tggctggacg ccgctgacgc gtgacggtgg tcagcaactt
     3901 gggcgtaagt tccagttgta tgacatgacc agcgaccgta gcgtcaattt ccgctaagtc
     3961 atgggaaagg tgccagtttt cgcaatcact ggcaccctca ttcttaatgg cctgttgagc
     4021 catcaggaac gcagttcacc cccggtttgc gatctggtgt gtaaaggcga aaacattctg
     4081 tcttcctgtc cgtcgatatc ggttgcagat ctggttgcct gagcgactgg gccatcattg
     4141 ggctggagac attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat
     4201 aagcaggaag aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca
     4261 cctttatcag gttacgtttc atttgttcca gaggaacatg gtcgattttt cgcgcattgc
     4321 tggtggctgg gaatcacctg aatgggtgat tttgaattac cggctttggt gcggtttgtc
     4381 ttgccggatg cgccgccagg cgcggcttat ccggcctacg ggtaggtata tccggctttg
     4441 gtggaggcgc gctccaaatc caggttgaac aaaacataca caaaaaatat agatctccgt
     4501 cacatttttg cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac
     4561 tctctcgtcc gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc
     4621 tgttgtgcct cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggctg
     4681 atgtgctatt cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt
     4741 actttctcta tttcgaattt atcgcgctga ttgtgaagta ctttcagtcc ggttttcact
     4801 tcccgctacg ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg
     4861 atcataaatc gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca
     4921 ccctgtggct gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact
     4981 ggcgcggcgt gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc
     5041 cggttcccag agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt
     5101 ccacataaat agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt
     5161 gtggaaatgg gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa
     5221 gtagttcgcc agccactggg ccgccaccgc gattgccagc gctttaccac gaatagcatt
     5281 cgggaagatt tccgacagca gtacccagca taccggaccc caggacatgg caaaggcggc
     5341 aacatagaac agcatcgaca gaagcgccac aatacccggt gcctgagtgt aaaacgcggt
     5401 accgaggcta aacataccga ttgccattcc gagtgcgccg ataatttgca gtggcttacg
     5461 accaaattta tccaccgtca taattgccag aacggtgaag gtgaggttga taactccgac
     5521 aataatggtc tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg
     5581 cgcgtagtac agcaccacat tgatgccgac aaattgctgg aggatggaga gcataacgcc
     5641 gatgacaatc acgcctacgc caaacatcag cagacgacca ccggttttgc ggccatgatc
     5701 cagagagtgt ttaatttcct gtactgcctg agttgcaagg gtgttgccca taattttgcg
     5761 caggatacct tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg
     5821 cacggtatac agcagcatta agaacagcag tgcagggata cattccgagg caaacatata
     5881 acgccagccg tcagtattca gccagctggc gtcgccggaa cgggcaataa aatagtttac
     5941 gcagtaaact aaaagctgcc cgaaaataat cgcaaactgg ttaaaagaga ccagtttccc
     6001 gcgaatatga gcaggagcaa gttccgcaat atacattggt gaaagcattg aggctaaacc
     6061 aacgccaata ccgccaataa tgcgataaat aacaaattcc gggacataac ctgccagata
     6121 aacaggcact gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc
     6181 tacaccagaa ataaaaaaca ggacagcagc aatcttaagt gaatcacgac gaccgaagcg
     6241 gctactgcaa taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggccac
     6301 gcaaaaccct aacagggagt tggcagcgga ttcacttaag ttttgtggag caacaaagac
     6361 ggtattgagt gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc
     6421 acctaatgta gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat
     6481 tcagacctgc cttagaccat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg
     6541 cagttaaggc aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta
     6601 attgtgataa ttatcaaaaa ttgatgaaaa caatactatt gccgtgactc agagcacgaa
     6661 agagaattat cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatggaaa
     6721 tattaaccgg cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc
     6781 cgaataacag tcacaagttg ggataactta agttgagggt gcagcggcat aacattggca
     6841 gaacaacatc tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga
     6901 gccagcaaga agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca
     6961 ccccacaggt agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagctg
     7021 tttacgtcac gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac
     7081 aggatgaaca ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc
     7141 tgccacggtg tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg
     7201 aaatagcctt tgatggtcca gacgtgcagc gcaatcccac ccagatacgc gaaaattacg
     7261 ccgccgtgag tattcaggcc aatgaatgga atgtactcac ccagacgatc aaacaacgca
     7321 tacaacgcga ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc
     7381 agcgtcgctt tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc
     7441 gccacaatgc caatcgcgga aatcccggcg acctttaccg agttccacag ccacagcagt
     7501 accgggaatg gcggtggcgt aatgcgacca tcagcctgtt ccacgctaaa acctaatgcc
     7561 agtttccagt gatcccagga gatttgctcc gggatcaggc taccggtcgc aaagttcccc
     7621 tggcgcagtg agatggcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt
     7681 agcagtagca ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc
     7741 atttttgtta tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca
     7801 gcgcacccac cagcaggaag atcagcgtgg ctattgctgc tgccagaccg aagtcctgac
     7861 ccccgccgcc ttcaaaagcg atgcggtagg tgtagttaac aagcaggtcg gtataaccgg
     7921 ctggcgtggt cgtgccaaga cgatccgggc cgccgttggt taacagttga atcagcacga
     7981 agttgttaaa gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca
     8041 gcggcagcgt aatcttaaag aagttctgga agggacctgc accatccatt gctgaggctt
     8101 catacaaatc gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat
     8161 aacccagcca ggtattgacg ataatcagca tcgttcgggc ggtggtcgga tcgctgaacc
     8221 aggcgggctt cacgccaaac agcgcgctca acatcatgtt gatctcaccg aagctctggt
     8281 taaacaatcc tttgaaaatc aagattgaaa tgaacgatgg caccgcgtag ggcagaatca
     8341 gcaggacgcg atagaccgct ttgccgcgca acgcttccca ctgcaccaga catgccagaa
     8401 ccatgccgac cgccaccgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga
     8461 aaatggcgag gaacggtttc tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc
     8521 agccggtggt cacggtgtaa ccggggctta gcttttcatc accccagttg ccgtcggcgg
     8581 taatggactg gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat
     8641 tcgtcaacgt gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca
     8701 gggagctcat catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct
     8761 gacgattctg ggtaatcacg cgtagattcg cgcgttcgcc ttcgggctgg gcggtcgttt
     8821 ctttcagttg cagtttttgc tcgccgccaa atttaaaagc gtcggaaagg taatttttgc
     8881 cggtttcgcc gtcactgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt
     8941 tataggtttt gcctgcttgc caggagcgat ctaacaacac ttcctgtgca cgttcaaaag
     9001 tcagctggtt agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga
     9061 agaggacgaa taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt
     9121 tacgattggc gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca
     9181 ggtattcccc ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac
     9241 ctagcactga ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca
     9301 tggggttctt cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc
     9361 ctgatgcgac actgatgcgt cttatcaggc ctacatgcgt ttcagttttg taggccggac
     9421 aaggcgttca cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt
     9481 ctttcagggc ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca
     9541 cggcatacca gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt
     9601 tttccatggt ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg
     9661 ccactgcacc cagcggtttg tctttattga ccgcttccag accttcgtca gtcagcagat
     9721 agttttcgag gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggcgc
     9781 tcagcacgcc aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac
     9841 cataattcac tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg
     9901 ctgtttcgcc tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt
     9961 gtttgttttt aataaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca
    10021 cgcccacgtc tttaatatcg tacttgccgt tttcatactt gaacgcataa cccccgtcag
    10081 cagcaatcag cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac
    10141 ctttcgcttt cagttcttta tccagcgccg ggatctcttc ccaggttttt ggtgggttcg
    10201 gcagcagatc tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca
    10261 gcttgccgtt gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt
    10321 tgtccggggt gatttcagcc aacaggccag attgagcgta gccaccaaag cggtcgtgtg
    10381 cccagaagat aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca
    10441 gtttatccgg atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga
    10501 cttcagcgag accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt
    10561 cgattttggc gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga
    10621 tgcgtgcacc tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa
    10681 acacctaaac ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta
    10741 cgccccaccg ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc
    10801 acataaaatc gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt
    10861 tagttcacag aagccgtgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg
    10921 tggaggattt aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg
    10981 atgacaggtt gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga
    11041 aagcctgggg cgaggtcgtg gtatcgaaag atatcaatct agatatccat gaaggtgaat
    11101 tcgtggtgtt tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg
    11161 ggcttgagac gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacactc
    11221 cgccagcaga acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt
    11281 cagtagcaga aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta
    11341 accaacgcgt caaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac
    11401 cgaaagcgct ctctggcggt cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg
    11461 agccaagcgt atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc
    11521 aaatgcgtat cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca
    11581 cccacgatca ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc
    11641 gcgtggcgca ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg
    11701 ccggatttat cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa
    11761 tcgatcaagt gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg
    11821 aaagccgtga tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac
    11881 tgccgagtga tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg
    11941 gcaacgaaac ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc
    12001 agaacgacgt ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc
    12061 gttgccatct gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg
    12121 tttaagcacc ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa
    12181 tgactcagga gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc
    12241 agcgggcgta atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg
    12301 tattggctgg acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag
    12361 taaataccgt cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt
    12421 gtggaaagag ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca
    12481 acagaatgac tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa
    12541 cctgatcgaa tggctgccag gttccaccat ctgggcaggt aagcgcttct accaacgtca
    12601 tgacgttcat atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga
    12661 aaacatcgat gttggcttcg gtaaactctc tctggcagca acccgctcct ctgaagctgg
    12721 tggttcttcc tcttttgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga
    12781 cgttttcgat gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg
    12841 tgtcgactac ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa
    12901 agacggctgg ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt
    12961 tgttgttcag tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc
    13021 tggcgtcgcg tttgataacg aaaaatttgc ctacaatatc aacaacaacg gtcacatgct
    13081 gcgtatcctc gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg
    13141 tatgtaccag gatatcaact gggataacga caacggcacc aagtggtgga ccgttggtat
    13201 tcgcccgatg tacaagtgga cgccaatcat gagcaccgtg atggaaatcg gctacgacaa
    13261 cgtcgaatcc cagcgcaccg gcgacaagaa caatcagtac aaaattaccc tcgcacaaca
    13321 atggcaggct ggcgacagca tctggtcacg cccggctatt cgtgtcttcg caacctacgc
    13381 caagtgggat gagaaatggg gttatgacta caacggcgat agcaaggtta acccgaacta
    13441 cggcaaagcc gttcctgctg atttcaacgg cggcagcttc ggtcgtggcg acagcgacga
    13501 gtggaccttc ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag
    13561 gcgtttacgc cgcattcgac aaccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc
    13621 tacaacggct gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca
    13681 ggttgtcatt atctgaaagg ggcgaaagcc cctctgatta tcgggtttag cgcgctattg
    13741 cctggctacc gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc
    13801 atcgccctct gtttatcagc agggttactg gcaagcgcgc ctggaatcag ccttgccgat
    13861 gttaactacg taccgcaaaa caccagcgac gcgccagcca ttccatctgc tgcgctgcaa
    13921 caactcacct ggacaccggt cgatcaatct aaaacccaga ccacccaact ggcgaccggc
    13981 ggccaacaac tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag cgtcccggca
    14041 aacattggcg aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgttttt
    14101 gcgccgaacg tgctgattct tgatcagaac atgaccccat cagccttctt ccccagcagt
    14161 tatttcacct accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc
    14221 ctgacaccgg cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat
    14281 ctccagcaga cgacccaact gctcgacccg gctaaagcct atgccaaggg cgtcggtaac
    14341 tcgatcccgg atatccccga tccggttgct cgtcatacca ccgatggctt actgaaactg
    14401 aaagtgaaaa cgaactccag ctccagcgtg ttggtaggac ctttatttgg ttcttccgct
    14461 ccagctccgg ttacggtagg taacacggcg gcaccagctg tggctgcacc cgctccggca
    14521 ccggtgaaga aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc
    14581 aaaaacgctg tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa
    14641 cgcctgggat cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa
    14701 ttacgcccca cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta
    14761 tgcttccttc ctgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa
    14821 tatccattat ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca
    14881 ttcaggctac ttttccaggt tattttattt ctgttatgca gaggttttat gataagtcat
    14941 atcctaaatt ctggcggcaa taactctttg atgaaacatg atgtggtgca aggaaataat
    15001 atagtagatc ttgatttact acgtaatttc
//
LOCUS       DAPHDX010000104        14914 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPHDX010000104 DAPHDX010000000
VERSION     DAPHDX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.105"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..521
                     /locus_tag="RH058_003285"
     CDS             <1..521
                     /locus_tag="RH058_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3138833.1"
                     /translation="AVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSI
                     NMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAI
                     ASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGD
                     VLSSLVMRVAQD"
     gene            602..1276
                     /locus_tag="RH058_003286"
     CDS             602..1276
                     /locus_tag="RH058_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3138834.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVSRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1292..3772
                     /locus_tag="RH058_003287"
     CDS             1292..3772
                     /locus_tag="RH058_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3138835.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3786..4820
                     /locus_tag="RH058_003288"
     CDS             3786..4820
                     /locus_tag="RH058_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV3138836.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4902..5240)
                     /gene="rcnB"
                     /locus_tag="RH058_003289"
     CDS             complement(4902..5240)
                     /gene="rcnB"
                     /locus_tag="RH058_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV3138837.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5459..6283)
                     /gene="rcnA"
                     /locus_tag="RH058_003290"
     CDS             complement(5459..6283)
                     /gene="rcnA"
                     /locus_tag="RH058_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV3138838.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6404..6676
                     /gene="rcnR"
                     /locus_tag="RH058_003291"
     CDS             6404..6676
                     /gene="rcnR"
                     /locus_tag="RH058_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV3138839.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6768..6864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6899..7687
                     /gene="thiM"
                     /locus_tag="RH058_003292"
     CDS             6899..7687
                     /gene="thiM"
                     /locus_tag="RH058_003292"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV3138840.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7684..8484
                     /gene="thiD"
                     /locus_tag="RH058_003293"
     CDS             7684..8484
                     /gene="thiD"
                     /locus_tag="RH058_003293"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV3138841.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8549..9367
                     /locus_tag="RH058_003294"
     CDS             8549..9367
                     /locus_tag="RH058_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV3138842.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            9419..10165
                     /locus_tag="RH058_003295"
     CDS             9419..10165
                     /locus_tag="RH058_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3138843.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10139..11104)
                     /locus_tag="RH058_003296"
     CDS             complement(10139..11104)
                     /locus_tag="RH058_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3138844.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11101..12105)
                     /locus_tag="RH058_003297"
     CDS             complement(11101..12105)
                     /locus_tag="RH058_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV3138845.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12102..13379)
                     /locus_tag="RH058_003298"
     CDS             complement(12102..13379)
                     /locus_tag="RH058_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138846.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13636..14688
                     /gene="fbaB"
                     /locus_tag="RH058_003299"
     CDS             13636..14688
                     /gene="fbaB"
                     /locus_tag="RH058_003299"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV3138847.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         3988 a         3496 c         3759 g         3671 t
ORIGIN      
        1 ccgctgtctt ttcttctttt tttatgagcg ctggagtatt tgctgcagac gttgataccg
       61 gaacattaac tattaagggg aatattgcag aatctccgtg taaattcgaa gcgggtggtg
      121 attcagtaag tattaatatg ccgactgtac caaccactgt ctttgaaggt aaagctaaat
      181 attctaccta tgatgatgca gtcggtgtaa ccagcagcat gttaaaaatt agctgcccga
      241 aagaagttgc tggtgtaaaa ctctcgttga ttaccaacga taaaataacc ggtaacgata
      301 aggcgatagc cagtagcaac gataccgtgg gttactatct ctatttaggt gataacagcg
      361 atgtcctgga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
      421 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
      481 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
      541 ctccaggtaa taaacatggc cgctatcccc atggcggctt tttaatttaa gaggcattaa
      601 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
      661 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
      721 gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
      781 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
      841 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
      901 agaaagcatt ttttatctga atgttcttga tattccacca aatagtccag aacaagaagg
      961 taagaatgca ctgaagtttg cgatgcaaaa cagaatcaaa ttgttttacc gcccggcggg
     1021 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaagtcgca gtggcaatgg
     1081 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     1141 tgtcaaagtc aattatgaaa ctataatgat tgccccctta gaaagtcaga gtgttaatgt
     1201 caaaagtaat aatgcaaata actggtatct gaccattatc gatgaccacg gcaactatat
     1261 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     1321 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     1381 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     1441 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     1501 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaatagaag ttatcaagcg
     1561 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     1621 tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt tcagtgtccc
     1681 gcaggcctgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     1741 tattaatgcg ttttatacct cttattatgt gagtcagtat tacagtgact ataaagcgtc
     1801 gggtaatagt aagagtacat atgtacgttt taacagcggg ttaaatttac tggggtggca
     1861 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     1921 taccctgtat ctggaacgtg gatttgccca acttctcggc acgcttcgcg tgggtgatat
     1981 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     2041 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     2101 gagtaatgcg ctggtaacta ttgaacagaa cggttttgtg gtttatcaga aagaggttcc
     2161 tcctggcccg ttcgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     2221 cagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcagtgcc
     2281 aaatatgctg caacccggcg tatcgaaata tgattttgcg gcgggtcgta gccatattga
     2341 aggggcgagc aaacaaagtg attttgtcca ggcgggttat cagtatggtt ttaataattt
     2401 attgacgctg tatggtggct cgatggtcgc gaataattat tacgcgttta ctttggggac
     2461 tggctggaat acacgcattg gtgccatttc cgtcgatgcc acgaagtcgc atagtaaaca
     2521 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     2581 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcatcgcgtg attatcggac
     2641 atttaatgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatgt
     2701 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tctctgccaa
     2761 tatgagccag tcattgccag aaggctgggg gtctgtgtcc ttaagtacgt tatggcgaga
     2821 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     2881 gcggataagc tatatcctcg cggcaagcca ggcgtatgac gagaatcatc atgaagagaa
     2941 acgttttaat atttttatat cgattccctt tgattggggt gatgacgtta cgacgcctcg
     3001 tcggcaaata tatatgtcta actcaacgac gtttgatgat caggggtttg cctcaaataa
     3061 tacgggatta tcaggaaccg ttggaagccg ggatcagttt aactatgggg tcaacctgag
     3121 ttatcagaat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     3181 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacaggctg gagccagtgt
     3241 ttcagggggc attgtcgcct ggtcgggggg cgttaatctg gcgaaccgtc tttccgaaac
     3301 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     3361 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     3421 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     3481 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     3541 ctggtttata aaagcgttaa gagcggatgg gcaaccatta atgtttggtt atgaagtcaa
     3601 tgatatccat ggtcataata ttggcgttgt cggtcaggga agccagttat ttattcgcac
     3661 caatgaagta ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     3721 aatcaccttc ggtaaagaga ttgatgaaag tagaaactat atttgccagt aatagtcagg
     3781 tggttatgaa tattcgtata atgttattta tagtaatgat gatggttatg cctgtgagct
     3841 atgcggcatg ttatagtgag ttatctgttc agcacaattt ggttgttcag ggggattttg
     3901 cacttactca aacacaaatg gcgacatatg agcataattt taatgattcg tcatgcgtga
     3961 gtacaaatac tatcacccct atgagcccgt cggatattat tgttggactt tataacgata
     4021 ccattaaatt aaatttacat tttgaatgga ccaataaaaa caacatcacg ttatcaaata
     4081 atcagaccag tttcaccagt ggttattcgg taacggtgac acctgcggcc agtaatgcaa
     4141 aagtgaatgt ttctgcgggg ggcggcggtt cagtgatgat taatggagtt gcgacattat
     4201 ccagtgcttc atcatcgaca cgcgggagtg ccgcagtaca atttctactg tgtttattag
     4261 gtggcaagtc atgggatgca tgtgtaaata gctacagaaa tgcattggca caaaatgcag
     4321 gtgtctattc ctttaatctg acattgtcat acaacccgat aaccacaacc tgcaaaccgg
     4381 acgatttatt aattacttta gacagtattc ccgtttcaca attaccagcc acaggtaaca
     4441 aagcaacaat aaatagtaaa caaggggata ttattctgcg ttgtaaaaat ttattaggtc
     4501 aacaaaatca aacatcacgg aaaatgcagg tgtatttatc aagttctgac ttgttaacca
     4561 acagcaacac aatactgaaa ggtgcggaag ataatggcgt aggatttatt cttgaaagta
     4621 atggttcgtc agtcacactt ttaaatatca ctaacagcag taaaggatac acaaatttaa
     4681 aagaaattgc ggcaaagtca aaacttacag atacaacggt ttcaattccg ataactgcca
     4741 gttactacgt ctacgataca aacaaagtta aatctggcgc actggaggca accgcattaa
     4801 tcaacgtgaa atacgactaa taacaagcaa gacgagcaag tggctaataa taaaaaataa
     4861 caaggtgtct gcatttcccc ctcatgatga gggggctttt tttagcgatg ataaaaaatc
     4921 tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca
     4981 cccatatagg tccagtgcgt cccggcatca ggcgcgggca ggttacgtaa ctgccactgc
     5041 ttaatgttgt actcatcggt ccggtacatc tctgggacgg tatcgcctgg tttgaaatgc
     5101 ttaaagtcag caatgaaact gcttaactca tacttagaaa ttcccgaggt attggtcgaa
     5161 cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca gcaaaagcgc acccagcaac
     5221 atcttatttt taatggtcat aaactctcca ctttggggat ctgtaatatc agtcgcatat
     5281 ctttcctgcc atacaatata actgacaagc gatattttgt tagtgatccc atgatttcgt
     5341 aaaaagtgta aatgaaagga tgccctaaaa gcatccttag cgagcagaga gtcagcggaa
     5401 atagctgatt taagaatcat cctgggctga agataacatg caagcctaaa ggattgagtt
     5461 atcgcattat gcccatgaag ccatgtacgc ccatatacac accgactaag ccaatcaaca
     5521 gactggaaaa atagggggcg cgtttagcga gagtgttaaa tccgctccag cgttttgcga
     5581 cctgttgaac gctgattgct gcgccaacgc ctacggtgac aagcgttaac gccaggccaa
     5641 tgctgaaact gacgaccagt gttgcgccca gtgtcagggc tttcaactga atgcatatca
     5701 acagcacggt aattgctgcc gggcagggga taaggccacc ggttaagcca aataacaaaa
     5761 tttgccagtt ggtgacctct ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc
     5821 gtgcatgggc atcctgatac tcgccatgtt cgtgatggtg atgatgtcca tggtcgtggt
     5881 gatcttcgtg atcgtggtga tgatgttcat gatcatactc gtgcatattc tccagccagt
     5941 tgcgttcgcc gcgccaggta cgccagaaca tccagaacgc ggtgccaata atgatcactg
     6001 cggaaatcag ctggagccac ggttctgctg attgagcagt aaagcgcttg ctgatcacca
     6061 tcccgccaaa ggcaattaac cagaccactg cggtatgcga aatagttgct gccagtccga
     6121 gcatcaccgc ttgtttaatg gtgcctttga tggcgatgat aaacgccgcc atcatcgttt
     6181 ttgagtgccc tggttccagg ccatgaagcg caccaagtaa gatggcgctg gggatgaaga
     6241 accaagcgtt tccttgctga agaagagttg taaattcggt catgataata attcttagta
     6301 ttaattcggc aatctgattc tactcccccc cagtacctga tactaccccc cagtagatta
     6361 atagtgctat gatttttcat attcttgtta accaggtgtt gccatgtctc atacaatccg
     6421 tgataaacag aaactgaaag cgcgtgccag taagattcag ggccaggtcg tggcgctcaa
     6481 gaaaatgctc gacgagccgc acgaatgcgc tgcagtttta caacagattg ctgctatccg
     6541 tggcgcggta aacggtctga tgcgggaagt gattaaaggt catctgacgg aacacatcgt
     6601 tcaccagggg gatgagctaa aacgtgaaga agatctggat gtcgttctga aggtgctgga
     6661 ttcatatatc aaataattta ttaacgcgat tgtaaaactg ccgtttttcc tcgtttacaa
     6721 cgcgtgcgct ggacattacc atcctcctct gcgatttatc atcgcaacca aacgactcgg
     6781 ggtgcccttc tgcgtgaagg ctgagaaata cccgtatcac ctgatctgga taatgccagc
     6841 gtagggaagt cacggaccac caggtcattg cttcttcacg ttatggcagg agcaaactat
     6901 gcaagtcgac ctgctgagtt cagcgcaatc tgcgcacgcg ttacaccttt ttcaccaaca
     6961 ttcccctctt gtgcactgca tgaccaacga tgtggtgcaa acctttactg ccaatacctt
     7021 gctggcgctc ggtgcatcgc cagcgatggt tatcgaaacc gaagaggcca gtcagtttgc
     7081 ggctatcgcc agtgccttgt tgattaacgt tggcacactg acgcagccac gcgctcaggc
     7141 gatgcgtgct gccgttgagc aagcaaaaag ctctcaaaca ccctggacgc ttgatccagt
     7201 agcggttggt gcgctcgatt atcgccgcca tttttgtcat gaacttttat cttttaaacc
     7261 ggcagcgata cgtggtaatg cttcggaaat catggcatta gctggcattg ctaatggtgg
     7321 acggggagtg gataccactg acgccgcagc taacgcgata cccgctgcac aaacactggc
     7381 acgggaaact ggcgcaatcg tcgtggtcac tggcgaggtg gattatgtta ccgatggcca
     7441 tcgcgccgtt ggcattcacg gtggcgatcc gttaatgacc aaagtggtag gaactggctg
     7501 tgcattatcg gcggttgtcg ctgcctgctg tgcgttacca ggcgatacgc tggaaaatgt
     7561 cgcatctgcc tgtcactgga tgaaacaagc cggagagcgc gcagtcgcca gaagcgaggg
     7621 gccaggcagt tttgttccac atttccttga tgcgctctgg caattgacgc aggaggtgca
     7681 ggcatgaaac gaattaacgc tctgacgatt gccggtactg atcccagtgg tggtgcgggg
     7741 attcaggccg atcttaaaac cttctcggca cttggcgctt atggttgctc agttattact
     7801 gcactggtgg cgcaaaatac ccgtggcgta cagtcggtgt atcgcattga gcctgatttt
     7861 gtcgccgccc agctcgattc ggtgttcagc gatgtgcgaa tcgacaccac taaaatcggt
     7921 atgttggcgg aaaccgatat tgttgaagcg gtggcagaac ggttgcaacg ttatcagatc
     7981 caaaacgtgg tactcgacac cgttatgctg gcaaaaagcg gcgacccgct gctttcacct
     8041 tcggcggttg ctacgctgcg cagtcgatta ttgccacagg tttcattaat aacgccaaac
     8101 ttgcccgaag ctgccgcctt gctcgacgcg ccacacgcgc gcaccgaaca ggaaatgctg
     8161 gaacaagggc gatcgctgtt ggcgatgggc tgtggcgcag tgctaatgaa aggtggtcat
     8221 ctggatgatg agcaaagccc ggactggctg tttacccgcg agggtgaaca acggtttacc
     8281 gcaccgcgca ttatgaccaa aaacacccac ggcactggtt gtacgctctc tgcggcgttg
     8341 gctgcactac gcccgcgcca tacaaactgg gctgacaccg tacaggaggc aaaaagctgg
     8401 ctttcatcag cattagccca ggccgacacg ctggaagttg gtcacggtat tggtccggtt
     8461 caccacttcc acgcctggtg gtgagcaccg tactatagct gctttggtta ttgtcagcct
     8521 ttggcactga cattcaggga tgaaaccaat gcagctaaga attaccagtc gaaaaaaact
     8581 cacctctcta ttatgcgctc ttgggctaat ttccatcgtt gcaatttatc cgcgtcagac
     8641 ggtgaatttt ttctattcga cagcagttca gattacagac tacatccatt tctacggtta
     8701 tcgcccggtt aaatctttcg ctattcgcat tcctgccagt tacaccattc acggaataga
     8761 tgtttctcgc tggcaggagc ggatcgactg gcagcgggtg gcaaaaatgc gcgacaacgg
     8821 tatccgctta cagtttgctt ttattaaggc gacggaaggc gaaaagctgg tggacccgta
     8881 tttttcgcgt aactggcaac taagccgcga aaatggcctg ctgcgcgggg cgtatcatta
     8941 tttctccccg tcggtagcag cgccggttca ggcacgatta tttctgcaaa cggtggattt
     9001 ctcacaaggc gatttccctg ccgtgctgga cgtagaagaa cggggaaaat tatcggcaaa
     9061 agaattacgc aagcgggtaa gtcagtggct aaaaatggta gaaaaacgca cgggaaaaaa
     9121 gccgattatt tactcaggag ccgtttttta tcacacgaat ctggcgggct attttaatga
     9181 atatccgtgg tgggtggctc actattatca acgtcgtccg gacaatgacg gaatggcctg
     9241 gcgcttctgg cagcattccg accgtggaca ggtagatggc atcaatggtc cggtggattt
     9301 taatgtgttt aatggcacgg tggaggagtt gcagggattc gttgatggga ttaaagaaac
     9361 gccttaaata agaagcagtg atatactccc accagactcc ctggcaggaa aaagacaaat
     9421 ggaacaagcg catacccagc tgattgccca actgaacgag cgtattttag cggcggataa
     9481 cacgccgctt tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca gcggggtgct
     9541 ggagcatggc aatattttgc ccggtgagcg tgatttaagt cagttaaccg gcgtttcgcg
     9601 cattacggtg cgcaaggcga tgcaggcgct ggaagaagag ggtgtggtga cgcgttcgcg
     9661 cggttacggc acgcagatca acaacatctt cgaatactcg ttgaaagaag cgcgaggttt
     9721 ttctcagcag gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta acaagcgtgt
     9781 cgtgaaatgt cccgaagaag tcgcgcagca gctggcggtc gaagcaggaa gtgatgtctt
     9841 tttgcttaag cgtattcgct atgtcgatga agaagcggta tcgattgagg aatcgtgggt
     9901 tccggcgcat ttaattcatg atgttgatgc catcgggatt tcactttatg actatttccg
     9961 cagccagcac atttacccac aacgtacgcg ttcccgcgtt agcgcccgga tgccggatgc
    10021 cgagtttcag tcacatattc agttagatag caaaataccg gtgctggtga tcaagcaagt
    10081 ggcgcttgac caacagcaac ggcctattga gtacagcatt agccactgtc gcagcgatct
    10141 atacgttttt gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca ccgccccgat
    10201 gcccgacaac ccacgacgcc actgcattgc ccagtaatac ggcatccgcc agtggcagac
    10261 cagaggccag cccggcaagt acgccacctg catgactgtc gcccgcccca atggtgtcta
    10321 caacttgcgt tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa taccatgcac
    10381 cctctttatc gagacgaacg atcaacggcg cggcaaattt ctcctgccat tgcttgccaa
    10441 gtgttgttat ctctgcggat aaagcaaaac gttcggcggc aatctcagcc tcttgacgat
    10501 tgagtgatac taaaggtcga caggccatga tccgcgccag taatgcgtcc gggatatcgc
    10561 cgatacgtgg gccaaaatcg ataaacggcg tcacgtcttg cagcttttcc agccattcca
    10621 ctaacaattc gccgcagggc gaggccagtt gataaccgga aaaatagagc aggctgccag
    10681 gcgcaacggt taatcgcgcc agccactggc gattccactg attttccaca ccgctgaatg
    10741 acataaaagt gcgttcgcca tccggctcaa ccagcgccag acaccagccg ttatcgcctt
    10801 cggcgttatc gatcaggctg attaacccct ctttcgccat ccggttgcga ataatctccg
    10861 cccacacgcc ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg cgctttaacg
    10921 ccacggcaat attcagtgcg cagccgccaa cgttaacgct ctgctgcttc agttcgatat
    10981 cgcacccacg ccaggggagg gcataagcgt cggcgatcac atcaatgacc gccgcgccga
    11041 cgaccatcac cggctgacgc gaggttaatt caggcagcag cgtgtgtaat cgagcgccgc
    11101 tcataccgcc tcccgttgtt gacgatattt cgccagcgct gtggcatagc ggttgaaatc
    11161 aagctgattt accgcatcca gttccgcctt taatgcagga tcgatagcgt taacgccatg
    11221 caacgcgccg caaatcgccg tcgccatagc gccgatggtg tctgtgtcgc cgccaaggtt
    11281 agcgcacagg acggcgcagc gattcgggtc ggtttgtgcc agttcaacca gcgcaatggc
    11341 gcacggaacg gactcaatag tgctggtacc tgcgccaacg acctggtaaa gctgttcgct
    11401 ggcggattcg gtgccgtcgg cattgcgcac aattttcagc gcaatctcca gacgtgctgc
    11461 cagtgaggcg ctgaaggtag tgatgcgttt ttgctgtgca tgtcgcgcaa ttgaaggcag
    11521 ggaatcaaca atcgctgacc agctttctcc gtcaatggca cgagaaatcg cccatgcgat
    11581 gactaccgca cctgcaactg ccagatcgga tttatgggtc gggctcgacg ccagcgccac
    11641 atcatcaata aaggaatcaa catcacgcgc cggaagcaaa caacctaatg gcgagacgcg
    11701 catcgctgcg ccgttggtca cgccgttgtt ttccagttca gcaacgggtt taccgtcgcg
    11761 aatggcgtta agcgcaatct tcgaggtcgg acctagtacg tttttgttaa aggcgtcgaa
    11821 acgcagcgcc cagtcgagaa tattacgccc aatcagatcc ggatcgatct tgccttcacg
    11881 ttccagtaac gcatccgcca gacacagcgc catcgaggta tcgtcggtga attcggcgcg
    11941 gttaaaataa caggccgcgt tattctcctt tggtccaggc agaaaacggt caatccagcc
    12001 aaagtgtgct ttaacgcggc tgcgtggcca aagctcggag ggcatcccca tcgcatcccc
    12061 taacgcctgc ccataaagag caccgagaat acgttctgtt ttcatttaac ttccccttgt
    12121 gtcaacgcaa tatcgcgatc atcgaccttg atagccgtaa tttcgttgtc ggattcgcgg
    12181 aaaaaaatca tgaacagcac ggcgataatg gcaatcatca ccgcgccgaa agtccacatc
    12241 ccggaccagt tgaaagtcag cccgtttacc ggttccggat aagcgaacat cttttccatc
    12301 atcacaccgc caagacgata gccgagcaaa ctgccgaagc cctggcagca gagcgtgatc
    12361 agcccctgcg cggcggtacg catatgcacg ggggcttttt tatcgacata gatgtaagcg
    12421 gtaacgtagt aaaaatcgta acttacgccg tgaagcaaaa taccgaggaa cagtaacgcg
    12481 taggtgaaat attcatccgc actaccgtaa ataaagaagc catagcggat cgcagcggtg
    12541 accagaccaa gcaataatac ctttttgata ccaaagcgtt tagtgaaaaa cggcaatgcc
    12601 agcataaaga agatttcaga gaactggccg agcgtcatcc agccggtggc gtttttcatg
    12661 ccaacttcgg tcagataacc gttggcaaag atgtaataga acgccagtgg catcgcaaac
    12721 aggaatgaac agaagaaaaa gacgaggaag tttttatcgc gcagcaggat cagcgcatcc
    12781 aggccgagca tgactttaat gtccattttg ccggtgcttt ttggcggcgt gtcgggcagg
    12841 aaaaacgcaa acacaccgag cagagcagaa cttccggcgg taatcagcag cgggatgtta
    12901 gtcggtgaga tatcggcata ccccagcatt tgcggcaaga aaccacatgc cagaccagag
    12961 gcaatccagc cgatagtgcc catcacacga atgcgcggga aatcacgctc cacatccggc
    13021 acgttggcaa aagcgatact gttagtcagc gcaatggtcg gcatataggt tagagagtaa
    13081 gccagcagta acggaaagaa cccggcaaaa gtggtctgtt gcgcagcgaa atacatcagc
    13141 agcgcgcctg cgaacatcaa taccgccagc actttttgcg ccgagaaaaa gcggtcagtg
    13201 atggagccaa ccagaattgg cgataggatc gccgcaatgg cggtacaggc atacgaccag
    13261 ccaatttctc cggcactaaa accgctttta cttaaccaca accacaatgg cacaaaccac
    13321 gcgccccaga taaaccattc aacaaacatc atgaacgaca gctttgctgt tgttttcatc
    13381 ttttaattcc ttcatgacag gtaatgtaag gtacgcgatg acaataccac ttaataatac
    13441 cttttaaata cctttgcggt gaaaacttga ccgcgctaac attttttctg atgaatcgag
    13501 ccaacagaaa acgctgaaaa aacatccaaa agatggaaaa actcgcccgt aacgaccagg
    13561 attatgattg cccgcaaaag gcgggccagg acaatccata gccgatatcc aatcggaatt
    13621 tacgggagca tagtaatgac agatattgcg cagttgcttg gcaaagacgc cgacaacctt
    13681 ttacagcacc gttgtatgac aattccttct gaccagcttt atctccccgg acatgactac
    13741 gtagaccgcg taatgattga caataatcgc ccgccagcgg tgttacgtaa tatgcagacg
    13801 ttgtacaaca ccgggcgtct ggctggcaca ggatatcttt ctattctgcc ggttgaccag
    13861 ggcgttgagc actctgccgg agcttcattt gctgctaacc cgctctactt tgacccgaaa
    13921 aacattgttg aactggcgat cgaagcgggc tgtaactgtg tggcgtcaac ttacggcgtg
    13981 ctggcgtcgg tatcgcggcg ttatgcgcat cgcattccat tcctcgtcaa acttaatcac
    14041 aacgagacgc taagttaccc gaatacctac gatcaaacgc tgtatgccag cgtggagcag
    14101 gcgttcaaca tgggcgcggt tgcggttggt gcgactatct attttggctc ggaagagtca
    14161 cgtcgccaga ttgaagaaat ttctgcggct tttgaacgtg cgcacgagct gggtatggtg
    14221 acagtgctgt gggcctattt gcgtaactcc gcctttaaga aagatggcgt tgattaccat
    14281 gtttccgccg acctgaccgg tcaggcaaac catctggcgg caaccatcgg tgcagatatc
    14341 gtcaaacaaa aaatggcgga aaataacggc ggctataaag caattaatta cggttacacc
    14401 gacgatcgtg tttacagcaa attgaccagc gaaaacccga ttgatctggt gcgttatcag
    14461 ttagctaact gctatatggg tcgggctggg ttgataaact ccggcggtgc tgcgggcggt
    14521 gaaactgacc tcagcgatgc agtgcgtact gcggttatca acaaacgcgc tggcggaatg
    14581 gggctgattc ttggacgtaa ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt
    14641 aatgccgtgc aggacgttta tctcgatagc aaaattacta ttgcctgata cgcctcatac
    14701 gaacagcacc accccgtagg acggataagg cgtttacact gcatccggca acctatgcct
    14761 gatgcgacgc ggaagcgtct tatcatgcct acatagcact gccacgtatg tttacaccgc
    14821 atccggcata aaaacacgcg cactttgcta cggcttccct atcgggaggc cgtttttttg
    14881 cctttcactc ctcgaacaat tttcatattg tcgt
//
LOCUS       DAPHDX010000105        14788 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPHDX010000105 DAPHDX010000000
VERSION     DAPHDX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.106"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..341)
                     /locus_tag="RH058_003300"
     CDS             complement(<1..341)
                     /locus_tag="RH058_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138848.1"
                     /translation="MKIVFEVNGKKVPLKSLKYISKKDQLEVMKNWFFENFEDPANAC
                     PYESREGGYAYIYGGPYDASEELQSIFGQYVKSEYIEELVDELQTQCFDWSGNSNNID
                     DWYDDDIYDAVT"
     gene            complement(533..955)
                     /locus_tag="RH058_003301"
     CDS             complement(533..955)
                     /locus_tag="RH058_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV3138849.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEVKTGATRYLMS
                     VLARELVASGDVCNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRQNIICRECRKSEVMQRILSFYQGDVRYLLK"
     gene            complement(996..2066)
                     /locus_tag="RH058_003302"
     CDS             complement(996..2066)
                     /locus_tag="RH058_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HDV3138850.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNLGQPAGTTPEADSAYALKADSGAVQQVMTARPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            complement(2138..2563)
                     /locus_tag="RH058_003303"
     CDS             complement(2138..2563)
                     /locus_tag="RH058_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDV3138851.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(2547..2789)
                     /locus_tag="RH058_003304"
     CDS             complement(2547..2789)
                     /locus_tag="RH058_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138852.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            3181..3519
                     /locus_tag="RH058_003305"
     CDS             3181..3519
                     /locus_tag="RH058_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S24"
                     /protein_id="HDV3138853.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQNI"
     gene            3812..3967
                     /locus_tag="RH058_003306"
     CDS             3812..3967
                     /locus_tag="RH058_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV3138854.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4127..4345
                     /locus_tag="RH058_003307"
     CDS             4127..4345
                     /locus_tag="RH058_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138855.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANETRAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            4913..5101
                     /gene="dicB"
                     /locus_tag="RH058_003308"
                     /gene_synonym="ftsT"
     CDS             4913..5101
                     /gene="dicB"
                     /locus_tag="RH058_003308"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV3138856.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5098..5289
                     /gene="ydfD"
                     /locus_tag="RH058_003309"
     CDS             5098..5289
                     /gene="ydfD"
                     /locus_tag="RH058_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDV3138857.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            5383..7854
                     /locus_tag="RH058_003310"
     CDS             5383..7854
                     /locus_tag="RH058_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV3138858.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWIKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQEIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            7927..8178
                     /locus_tag="RH058_003311"
     CDS             7927..8178
                     /locus_tag="RH058_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV3138859.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8213..9493
                     /locus_tag="RH058_003312"
     CDS             8213..9493
                     /locus_tag="RH058_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDV3138860.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKHKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(9495..9623)
                     /locus_tag="RH058_003313"
     CDS             complement(9495..9623)
                     /locus_tag="RH058_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV3138861.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTCQLFDKY"
     gene            complement(9681..10700)
                     /locus_tag="RH058_003314"
     CDS             complement(9681..10700)
                     /locus_tag="RH058_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV3138862.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(10712..11926)
                     /gene="rspA"
                     /locus_tag="RH058_003315"
     CDS             complement(10712..11926)
                     /gene="rspA"
                     /locus_tag="RH058_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV3138863.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(12132..12458)
                     /locus_tag="RH058_003316"
     CDS             complement(12132..12458)
                     /locus_tag="RH058_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV3138864.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            12593..12934
                     /locus_tag="RH058_003317"
     CDS             12593..12934
                     /locus_tag="RH058_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV3138865.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            12969..13529
                     /gene="speG"
                     /locus_tag="RH058_003318"
     CDS             12969..13529
                     /gene="speG"
                     /locus_tag="RH058_003318"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV3138866.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13532..14242)
                     /locus_tag="RH058_003319"
     CDS             complement(13532..14242)
                     /locus_tag="RH058_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV3138867.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            14350..14655
                     /locus_tag="RH058_003320"
     CDS             14350..14655
                     /locus_tag="RH058_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV3138868.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         4134 a         3600 c         3324 g         3730 t
ORIGIN      
        1 gttacagcgt cgtagatatc atcatcgtac caatcatcta tattatttga gtttccagac
       61 cagtcaaaac attgtgtttg taattcatct actaattcct caatatactc agatttaaca
      121 tattgaccga agatggattg tagttcttcg ctcgcatcat acggcccgcc atatatgtaa
      181 gcatagccac cttctctact ttcatacgga caagcatttg ccggatcttc aaagttttca
      241 aagaaccagt ttttcattac ttcgagttga tcttttttgc ttatgtattt taaagatttt
      301 agtggaactt tctttccatt aacctcgaaa acaattttca cgatgtaacg ctcccttgta
      361 tcaattgcgc aactattata ccacagcgaa atacagggca taacctgcct ccttaaacta
      421 acataccatg agccagcaat gtccgctatt ggcacaaagc ggacaaccac gctagctcta
      481 ccctgtgcca caaaatgtca atttgcatct gaactaatgc actttaatct cgtcacttca
      541 ataaataccg aacatcaccc tgataaaacg acaatatgcg ctgcataact tcactcttcc
      601 ggcactcgcg acagattatg ttctgacgcc tgtcgtagcg acgtatttct ccgtcaggta
      661 atgaccagat aaggtccgga tcaaccgcag atggtttctt cagctttgcc cttgagagct
      721 ttttacgggc attttgccag tccttacgcg cctgttcaga cgggaataac ccgtaaccag
      781 agttgcatac atcgccgctg gcaaccagct ctcttgcgag aacgctcatc agatatcttg
      841 tcgcacctgt cttgacttcc agttgccgta acgtctcacg cccactctgg cgtacgagtt
      901 caacaacctg tcctttaatt ttttcccgct cttcttgtgt aaaaactttt gccacaagcc
      961 ctcctgaaaa ttacctcatg accagaaatt aacacttacc ccctgaagcc cggcggaatt
     1021 tcgttatccg gttcagaaat atgattcaca caacgctggt tgttcgtgcc gcttaccggg
     1081 agcaaccagg ggttctcaaa attccggtcc ggtccaaaaa acgtcgtcgc tcgctgaaca
     1141 aattccgttc ccgttttccc ggtagccgcc aagtatcttg cgtaacgcct cacgccatcc
     1201 agcatggcct ctggtggcac cccctcgcgt aatctggcct tccaggcact gaaagcggat
     1261 ttcttcgggt ttgccccagc acgcaacggg tactcccgcc agacctgttc gaacacatcc
     1321 ggataatcca ctcgtcccac agactgcccg gtgttttccg ggactacccg atcggcttcc
     1381 cgctgaatgg cggaatcggc ttcgggctgc tgcagttggt gtgattgctc cggccttgcg
     1441 gtcatcacct gctgcacagc gcccgaatcg gctttcagcg catacgctga atcggcttcc
     1501 ggtgtcgtgc ctgctggctg accaagattg acggtctgaa catcccctgc ctggttcgtg
     1561 gcgtttttta cgccatggac catagtgttt tgatcttctt gatctgtatc tttatctgta
     1621 tctttatctg tcgtgactcg tcgtgacatg tgcgtgacat ttcgtgacgc gccgtgacaa
     1681 tcgccatttt gttcccgctt tctttccctc tctcgctgcg ccctcttgcg ctctgcagga
     1741 gattttgcgg tttgcgaaat attgccgttg tcctctttaa gcacctggcg tttttcccat
     1801 ccagtgatta aatcaccatc aagtacccgc ccctgcatcg tctgcaaaat tgaatcaatt
     1861 acctcttctg tcacgtcgag cgcacttgcc aaatcttctg tcgtgacatc aatgtgacct
     1921 cgcgtgacat ttcgtgacgc gctcaccagg aggtggatat acactgccat cactgttgca
     1981 attggctgcc ctgacaccct ggcaattgtt cgccacttag ggtcatttgg catgtcatgc
     2041 cataatctga gccaggcgtt agccatactc acctcttctg ataccgaatc tttttactca
     2101 cgagttgccg gaagcgattc gatatggcta ttgtcagtca atgtactgcc acagcatttc
     2161 ctgccgggcc accacggttc atctgattga aaccggcgat tgccactgcg acaaaatcat
     2221 cagcgtctct caccagtcgc tcccgcgtct ccaccagctc ccgaaaataa gctgaactgt
     2281 ggctgcgcat tctggccacc agcaaaggtg gcattgcctt ttcgatcgct ggtaacaacg
     2341 cctgaatttt ttcaactgca tcaggggtgt ctttctctat ccagcggaaa attttctggg
     2401 tattgcgagc cagggcttcc ggatggctgt cgtcgtacag ttcaggaaac gtcataccca
     2461 actcaaaata agcctgggtt attccagctg ccggaacttt ttcgccatca ggacgcgccc
     2521 aggcattcat cgccatgcgg atgtgttcat gcttgatttt catgaatccc cccttggtta
     2581 gaaggcggat tatgatcaga accgggaatg acaaccgtcg gtatgtgtaa ctcatatttg
     2641 agcgccccgg cagtgactgc ctgaattagc aacgcccatt tccacggaac ctcttccccc
     2701 cacatgctga ctgtggtttt tgacgttcct agagctgcgg ctgttttaac aactccgcca
     2761 aaatagccta atacttctga ttttttcatg agtcgctcca taaaactgaa cgccaaaagt
     2821 ttaataatca aaaccaaaga aagtcaagaa acaaaaccat ctgtgtttta aaatcaaaac
     2881 atgaacaagc aaacaatatc tgaacgcata acccaacgta tgcatgcgct aaacctgaaa
     2941 ggcaaagacc ttgtcaatgc cactggcgca tcaaaaggct ccgtaagtca atggatgaac
     3001 ggtggaggag cgccgtcctc gcgttacata agttcactgg caaaaatatt gaaagtaaac
     3061 gaaaattggc ttcttaatgg aggagagtta aatacaggtg attcgcttga tctatcttta
     3121 ccgccgataa aaacggttcc gctactatca cttcagcagg cagcaagctg gagtgattat
     3181 atgaaaaatt cctcaataac ctcttgtgtg cagcttgtcg gagaaatccc ggccaatacc
     3241 tttgcagttg ttctagagag tgacagtatg tcaacatctg gtgggggagt ttccatccca
     3301 aatggttcaa cagtttttgt tgatcccgat cgaaccgtac aaccaggaaa tattgtcctt
     3361 gccttaccca aagggaccac aacacctgtc attcgtaaac tggagataga agggccggat
     3421 attcttttag tccccacgaa tcctcgctac ccttcaatta tgctggatga tctatcttgc
     3481 atattgggcg tatgctttaa aattcaacaa aatatttaac cgacctcatc tatttgatta
     3541 actgtatgcc atcgtggtga tggcttaaca gctgcctgct taaaatgttt tgataaaaaa
     3601 acattgacct gaaaagttca tttttctaaa cttcattcat tccctctccc caccccacag
     3661 aatgcagggc aatacttcta gttaccaggc agtggtcagg ggttaagtag ccagcccgag
     3721 gcgtaagaac atgacggcag ggttcaactt taataactat gcagcaggtt tttgttccgc
     3781 taccccggcg ttaaggggaa atgaggtcag catggatact atcgatcttg gcaacagcga
     3841 atctctggta tgtggcgtgt tccccaacca ggacggtacg ttcaccgcga tgacgtatac
     3901 caaaagcaaa acgtttaaaa ccgaaaatgg tgcccgtcgc tggctggaaa gaaactcagg
     3961 tgagtgatat ggatttcgac acaatcatgg aaaaggctta cgaagaatac ttcgaaggtc
     4021 ttgccgacgg cgaagaagct ctcagcttca acgaatttaa acaggcgctt tccagttcgg
     4081 caaaatctaa cggctgataa gcgaaacagc accgcgagga atcagtatgc agaaacgaga
     4141 acccgtcatc atcgcgccag actataccga tgatgaactt tatgagtgga tgcgccagaa
     4201 aattaatgca gcgcaggatt tgaaatgggc caatgaaacc agggctaagc aggctgaaaa
     4261 tctgtccgct ctggagcagg atatcaccaa tctggaaaaa gcagcggcat taagcattgc
     4321 cagaatgatt acatacccgc gttagtagct aatcaacaaa gctaaggtta gtaattaagg
     4381 agttctccac gggtgaggtg gagtgcgtgc gccggacacg ggtgagcatc cggcactgac
     4441 agtttactga aaggatattt ccctgaaaag tcagaccata acgcgaaagc gcacggcgag
     4501 gtagctggtt catagatagc ctgtcgttaa attttcgtcg accgtgcgct tccggttgtg
     4561 gcaacccgcg aaatggcgcg gcggtaagta tggcggggtt attccttccc cgttgaggac
     4621 accgggttgt caggttgacc atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta
     4681 tcaattttct ggtgacgttt ggcggtatca gttttactcc gtgactgctc tgccgccctt
     4741 tttaaagtga attttgtgat gtggtgaatg cggctgagcg cacgcggaac agttaaaacc
     4801 aaaaacagtg ttatgggtgg attctctgta tccggcgtta attgttaact ggttaacgtc
     4861 acctggaggc accaggcact gcatcacaaa attcattgtt gaggacgcga taatgaaaac
     4921 gttattacca aacgttaata cgtctgaagg ttgttttgaa attggtgtca ctatcagtaa
     4981 cccagtattt actgaagatg ccattaacaa gagaaaacaa gaacgggagc tattaaataa
     5041 aatatgcatt gtttcaatgc tggctcgttt acgtctgatg ccaaaaggat gtgcacaatg
     5101 aattcagcat ttgtgcttgt tctgacagtt tttcttgttt ccggagagcc agttgatatt
     5161 gcagtcagtg tgcacaggac aatgcaggag tgtatgactg cagcaaccga acagaaaatt
     5221 cccggtaact gttacccggt cgataaagtt attcaccagg ataataacga aatcccggca
     5281 ggtctttaaa acagttccgt aataaatatc cggtttcatt cttatatgcc agcaatggca
     5341 gggatttgtt catccttaaa tctgtcatga ggttaaaaca aaatgagtaa agtctttatt
     5401 tgcgccgcta ttcctgacga actggcaaca agggaagaag gcgctgtggc tgtagccaca
     5461 gccattgaag ctggcgacga acgccgtgct cgagcaaaat ttcactggca gttcctggaa
     5521 cattatccgg ctgctcagga ctgcgcttat aaatttattg tctgcgagga taaacctggc
     5581 ataccccgcc ctgccctcga ttcatgggat gctgaatata tgcaggaaaa ccgctgggat
     5641 gaggagtctg cttcttttgt cccggttgag actgaatccg atccgatgaa cgtcactttt
     5701 gacaagctgg cccctgaagt acagaacgct gtcatggtta agttcgacac atgtgaaaac
     5761 atcaccgttg atatggttat tagcgcacag gaattgttgc aggaagacat ggcaacattc
     5821 gacggacata tcgttgaagc gttgatgaaa atgccagaag ttaacgccat gtatccggag
     5881 cttaagttgc acgccattgg gtgggttaag cataaatgta ttcctggtgc taaatggccc
     5941 gaaattcagg cagagatgcg catctggaaa aaacgtcgcg aaggtgaacg caaggaaacc
     6001 ggaaaataca cgtctgttgt tgatctcgcc cgcgccagaa ccaatcaaca gtacactgaa
     6061 aattcaacag gaaaaatcag cccggtcatt gctgccattc atcgcgaata caagcagaca
     6121 tggaaaacac tggatgacga actggcctac gctctctggc ctggtgatgt ggatgccgga
     6181 aacattgacg gcagcatcca tcgctgggca aaaaaagaag ttatcgacaa cgaccgcgaa
     6241 gactggaagc gtatctcggc atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc
     6301 cagactattt ttggccttgt ccgtgagcgt ccgatcgaca ttcacaaaga tcccatagca
     6361 ctgaacaaat atatctgcga atacctgacg acaaagggcg tgtttgagaa tgaagaaaca
     6421 gacctgggca ctgttgatgt tctccagtca tcagaaacac aaactgatgc agtggaaact
     6481 gaggtatctg atatcccaaa aaatgaaacc gcgccggaag ctgaaccatc tgtagagcgt
     6541 gaggggccgt tctatttcct cttcgcagat aaggacggag aaaaatacgg tcgcgcaaac
     6601 aaactttctg gtctggataa ggcactggct gctggcgcca ctgaaatcac aaaagaagaa
     6661 tattttgccc gaaaaaatgg cacatacacg ggcttaccgc aaaatgtaga taccgctgaa
     6721 gattcagaac aaccagagcc gataaaagtt accgctgacg aagtaaacaa aattatgcag
     6781 gcagccaata tcagccagcc tgacgccgat gaactgcttg cagtatcacg tggtgaattt
     6841 gttgaaggga ttagcgaccc gaatgatccg aaatggatta aggggatcca gacccgcgat
     6901 tctgtgaacc agaaccagca agaaacggaa cagaacgacc agaaagcgga acaaaacagc
     6961 ccaaatacgc aacaaaacga gccagaaacg aaacaacctg aaccagtagt gcaacaggaa
     7021 ccggaaaaga tctgcaccgc ctgcggtcag agcggtggcg gcaactgccc tgattgtggt
     7081 gcggtgatgg gtgacgcaac ataccaggaa atattcgatg gagagaatca gcctgaagtt
     7141 caggaaaatg atccggagga aatggaaggc actgcgcatc agcacaagga gaacactggc
     7201 ggcaatcagc atcatgccag cgatagtgaa actggcgagg cgtcagatcc cttaattaag
     7261 gcaaacggtc atcataatct cacatccacc agcagagcgg ggattcatct gatgatcgac
     7321 cttgaaacca tgggaaaaaa tcccgatgcc ccgattatct caataggtgc aatatttttc
     7381 gatccgcaaa ccggagatat gggaccggaa tttagtaaga ctatcgatct ggaaactgct
     7441 ggcggagtca ttgatcggga caccattaaa tggtggctta aacaatcacg cgaagcgcaa
     7501 tctgccatta tgaccgatga aatcccgtta gatgatgcac tgttacaatt gcgggaattt
     7561 atcgacgaaa actccggtga attttttgtt catgtctggg gaaatggagc caacttcgac
     7621 aacacgattt tgcgccgttc atacgaacgg caggagatcc cctgcccgtg gcgttactac
     7681 aacgatcgcg atgtacgcac aatcgttgag ctggggaaag ccatagactt cgatgccaga
     7741 acggctattc cattcgaagg tgagcgccat aatgcacttg atgacgcccg ttaccaggca
     7801 aaatacgttt cagttatctg gcaaaaactg atcccgagtc aggctgattc ttaatgttca
     7861 actgtcgccg gttgtgactg gtattctgca accggcgctc gtctgatgta agagataaag
     7921 aaatcgatga gcgaagtaat catgattgtc tctcccggca aatgggtatc cgaagagcag
     7981 ttaattgcgc tgaaaggaat aaaaaaaggt acgttaaaaa aggcccggga aaaatcgttt
     8041 atggaaggaa gggaatataa gcatgtcgct catgacggta tgccatggga taacagtcca
     8101 tgcttttaca acctggaaga aattgatcgc tggattgagc gccaggcatc agcgagacca
     8161 agacgtcatc ttacttgact aaaagccaca ctaactaatg agagaagttg aaatgaaata
     8221 tccgacaggc gtggaaaacc atggagggaa attacgtatc tggtttgttt ataaaggcgt
     8281 aagagtcagg gaaaatctgg gggttcctga cacagcaaaa aacaggcgcg ttgcaggtga
     8341 actacgcgcc tctgtttgtt acgcaataaa aactggtgtt ttcgactatg caaaacagtt
     8401 tccctcctca cgcaatctgg aaaaatttgg tgaggcccga caagatttaa ccataaaaga
     8461 actggctgaa aaatttctgg cactgaaaga aactgaagtc gccaaaacat cactcaacac
     8521 ataccgtgcc gtcatcaaaa atatcctgag cataatcggt gaaaaaaatc ttgcctcatc
     8581 gattaataaa gaaaaattac tggaggttcg taaagagtta ctgactggat accagatccc
     8641 caaaagtaac tatattgtta cacaaccagg gagatcggct gtaactgtaa ataattacat
     8701 gacaaatctt aacgccgtgt tccagtttgg tgttgataac ggttacctgg cagataatcc
     8761 gtttaagggg atctcgccat taaaggaatc aagaaccatt ccggatcctc tttcgcggga
     8821 agaatttatc cgtcttatcg atgcgtgcag aaatcagcaa gcaaaaaatt tatggtgtgt
     8881 ttctgtttat actggcgttc gccctggtga gctgtgtgca cttggatggg aggacataga
     8941 tctgaaaaat ggaacaatga tgatcaggag aaatttagca aaagaccgtt tcacggtacc
     9001 aaaaacacag gcgggaacca atcgggtcat tcatcttatt aagccagcaa tcgacgctct
     9061 ccggagtcag atgacattaa cgagactgag caaagagcat atcattgatg ttcacctcag
     9121 agagtatggc agaacagaaa aacataaatg cacctttgtt tttcaacctg aagtgtcagc
     9181 gagagtaaaa aattatggtg accattttac cgttgactca ataaggcaga tgtgggacgc
     9241 agcgataaaa cgtgccggac tccgccatcg aaaatcatat cagtcgagac atacttatgc
     9301 ctgctggtcg ctgacagctg gtgctaaccc ggcatttata gcaaaccaga tgggccatgc
     9361 agatgcgcaa atggtatttc aggtatacgg aaaatggatg tctgaaaaca ataatgcaca
     9421 ggtagctttg ttaaatacac agttaagcga gtttgcccca accatgcccc ataacgaagc
     9481 aatgaaaaat taatttaata tttatcaaat agttgacacg tatgactctt gaaatccata
     9541 aattcaagcg cagtgcccag ccatcccgat actgctgctt tcaccaaatc cttagtgctt
     9601 ctttcgtgtt tttctattgt cataatggtt atctctaaaa aagaggtaag atgcgtacta
     9661 cttactcgcc gttattggta ttattcagaa aaagtgagta agactttgca gcaatgtttt
     9721 tgatcctgtt caaataaact aatggcatca gcaacatgct ggaaatcaaa cgtatgggta
     9781 attaattttt ctggtttaat taacccttta cttaaccagt cgataacaac cggaaattta
     9841 tttgcattta agcgtgaaga gaaaatagag agttcttttc cggtaattcc ttgctgaatc
     9901 acttcagacg gttcactgga gaagcccatc aatacaatac gtgccgctgg agaagccagc
     9961 gttacggcct ctttcaggat agaaggatga caagccgcat cgataattaa tgtcggcttg
    10021 atgccttttt cagcgaaaat ctcgccaagc ggtgtctggc tgttattaat cgcccagtct
    10081 gccccgctct ctttcgcttt ttccagtcgt tcatcaatgc gatcggcaac aatcacattt
    10141 ttaacgttat agacgccttt taatacctga acgatcgtca ggccgattgg accggcacca
    10201 taaaccagaa cggtatcatt ttcagtcggt tgaccatgac cggtaacgtt agccgcaatg
    10261 gtaaaaggtt cgatcatcac cgcatattga tcggccactg cttcaggaat tttccacgca
    10321 ttttttgccg gaaccacggc atattcactg aaaccaccgt cagcgtgcac acctaataca
    10381 gcaagtgtcg tacaaacgtt cggcttacct atagagcacg gatagcaatg cccacagctg
    10441 accaccggat cgacagcaac gcgttcaccg actctggcgc tttccacacc ttcacccacc
    10501 gcatcaatga cgccaaagaa ttcatgacca atgacgcgcg gatatttcgc aaaaggatta
    10561 tgcccacggt aaatatggct atctgaacca caaattccgg caagtttcac ttttactcgt
    10621 acttcacccg ctgacggggt gggtatttca cgttcgataa tcgacagttg attcggtttt
    10681 tcaattaaaa tgcttttcat taccttactc cttaccagtt ccacagcgtg ccatcttcca
    10741 gacgtgcgac tggcagataa gcaggttcat agggatattt cgccgccagc ttttcatcga
    10801 attcgatgcc aagacccggt ttgtctcccg gatgcatata gccgttatcg aaagtccagt
    10861 tgtgcgggaa gacttcgagc atttgttcgg aataacccat gtactcctgg acaccgaaat
    10921 tggggaccca cagatcaaag tgcagcgccg cagccatgca gactggtgac aaatcggaag
    10981 gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc cggcgcatac
    11041 cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt tgctcttcaa
    11101 tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg ggtgtgacgg
    11161 tatgttggcg aatgagacgg aagcattcct ggttttccgc aggcgtcggg tcttccatcc
    11221 agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata ggcgttaagc
    11281 gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt accgcgtcaa
    11341 acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct tccggccact
    11401 gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg taggtggttt
    11461 tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc tgatgacggg
    11521 cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa accatcaccc
    11581 cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct ttggctttaa
    11641 tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga ccgcgacgcc
    11701 agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca tcgcgaccaa
    11761 taagctgcgg acaaaggtga tcctgcaaat aagaggccac ggaaagctca cgtccattga
    11821 gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat gtgacgaaat
    11881 tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc gattccttgc
    11941 atcgcttgtc gtgatgcatg aaatctacgc aactgagcta ctaccataca agtatgaaga
    12001 tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga atggttgaca
    12061 aaagatgaaa tagaatacct tttgtcagct gacacttcct cttatcttat tgataaaatg
    12121 gatttatgtt cctacgtgcg cccccagccc gcaacaatga tcaacatgcc gcaaagcgca
    12181 atcaacgcac ccgtccagtc ataaagagtc agtttcacgc catccacaac gcgcagccac
    12241 atcaacgccg tgcagacata aacgccacca taagccgcgt aaacacgccc actcgccgct
    12301 ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa tccccgccgg aagcaacagc
    12361 cagatactgg cgtttcgttt taaccacaac cagggcagaa agcatccaat aatttcacac
    12421 agcgcagtag caaaaaatag taacgttgtt ttaatcatct ttgtcactta ttgacatcat
    12481 gtatagttat agggcgacat aatatcatca atataaacac cctcctggta cgttttgcgt
    12541 tcgcagtgga tggtgtagaa tctcctttaa tcattcatac agggaatgaa ttatgaaaat
    12601 cactctcagc aaacgaatcg gcctgctcgc ttttctgctg ccttgcgcac tggcactgag
    12661 cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca gtgcacaaag
    12721 ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc tgcgtcagaa
    12781 agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg ataaccgcga
    12841 taaatgtgag caaagcgcca acatcaatgc ctactgggag ccaaatactt tgcgctgcct
    12901 ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa ggaatgtaag
    12961 gacacgttat gccaagcgcc cacagtgtta agctacgccc gctggagcgt gaagatttac
    13021 gctatgtaca tcaactcgac aataacgcca gtgtgatgcg ttactggttt gaggaaccct
    13081 acgaagcctt tgttgaactc tctgatctgt atgataagca tattcacgat cagagcgaac
    13141 ggcgctttgt ggtggaatgt gacggcgaaa aagccggtct ggtggagctg gtggaaatta
    13201 accatgtaca tcgccgggca gaatttcaga taattatctc cccggagtat caggggaaag
    13261 gtctggcaac ccgtgccgcc aaattagcaa tggactatgg ctttaccgtt ctcaatctct
    13321 ataagctgta tctgatcgtt gataaagaga atgaaaaagc gattcacatt taccgcaagc
    13381 ttggcttttc ggttgaaggt gaattgatgc acgagttctt tattaatggt caatatcgta
    13441 atgccattcg catgtgtata ttccagcatc agtatctggc agagcacaaa acaccgggtc
    13501 agactctcct gaagccgacc gcacaatagc attaataata atcgatcgta tttttgatgg
    13561 tgtaaacccg ttcgacggcg ggttttactc cttcatcaac aatgattagc tgacagtcca
    13621 ccggattagc gtgactgtca tattcacagc tctgttttac attaccaacc cgttgattat
    13681 tcagtaaagt aaccgctgtg taatctaatt ttttgatcgg atccgttgat ggcgtggcgc
    13741 tgacagataa tgttttgtcg ttacttttcg tggttttacc cagcggataa ccctgatcat
    13801 catagcgata ttccatctgc atttgtttgc tgctggcttt aatcacgaag ccattatcat
    13861 cggtttccca actcaccccg gcagaaggta attctgctag ctggcatttt ccctgtaaac
    13921 gtactctctt ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt accagagcga
    13981 ccacggtatt attttccaga tccagtaatt cgagtgaatc aaaacagcct tcttccgaca
    14041 aagtcccaga aacacgtttc gtcacttcac cttgctcatc cattaatgtc tgagtgaaat
    14101 cttttaccgg accacgcagc ggatcaaaat cgaattcatt agagaaactg gccatctcag
    14161 gggtaaatga aagtgttacc tctgtgcggt cacatcctgt gaggaatatc gcgagtaagc
    14221 atggtagtaa tttgtatttc acaacagtca ccagagagta gagatgattc tcaatcatag
    14281 tagcaaatac agtactttac acgttaaatg ctatgcttaa agaagttatc ttcgcgtaag
    14341 gagcttatga tgaaactctc aacctgctgt gccgcgcttc tgctcgccct cgcctcaccc
    14401 gcggtactcg ccgcgccggg atcctgtgag cgcatacaga gcgatatatc acagcgcatt
    14461 atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa tgaccaggtt
    14521 gatcagcctg attcccaggt cgtcgggcat tgtgctaatg atacgcataa aattctctac
    14581 acccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga tggcgcgcct
    14641 gccgaaccgc agtaatacat tatcgactga acgccggata cgacacatcc ggcgatttga
    14701 acatacaaca taatcccacc ttattactca tacccttcta ttgatatgga ttaataattc
    14761 ttaacccaaa atgggtagac tccctcta
//
LOCUS       DAPHDX010000106        14759 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPHDX010000106 DAPHDX010000000
VERSION     DAPHDX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.107"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(146..2413)
                     /gene="adiA"
                     /locus_tag="RH058_003321"
     CDS             complement(146..2413)
                     /gene="adiA"
                     /locus_tag="RH058_003321"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV3138869.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2612..3520)
                     /gene="melR"
                     /locus_tag="RH058_003322"
     CDS             complement(2612..3520)
                     /gene="melR"
                     /locus_tag="RH058_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV3138870.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3803..5158
                     /gene="melA"
                     /locus_tag="RH058_003323"
     CDS             3803..5158
                     /gene="melA"
                     /locus_tag="RH058_003323"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV3138871.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5261..6682
                     /gene="melB"
                     /locus_tag="RH058_003324"
     CDS             5261..6682
                     /gene="melB"
                     /locus_tag="RH058_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV3138872.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6821..7450)
                     /locus_tag="RH058_003325"
     CDS             complement(6821..7450)
                     /locus_tag="RH058_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV3138873.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7573..9219)
                     /gene="fumB"
                     /locus_tag="RH058_003326"
     CDS             complement(7573..9219)
                     /gene="fumB"
                     /locus_tag="RH058_003326"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV3138874.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASVHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9297..10637)
                     /gene="dcuB"
                     /locus_tag="RH058_003327"
     CDS             complement(9297..10637)
                     /gene="dcuB"
                     /locus_tag="RH058_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV3138875.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11208..11927)
                     /gene="dcuR"
                     /locus_tag="RH058_003328"
     CDS             complement(11208..11927)
                     /gene="dcuR"
                     /locus_tag="RH058_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV3138876.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11924..13555)
                     /gene="dcuS"
                     /locus_tag="RH058_003329"
     CDS             complement(11924..13555)
                     /gene="dcuS"
                     /locus_tag="RH058_003329"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV3138877.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13736..13966
                     /locus_tag="RH058_003330"
     CDS             13736..13966
                     /locus_tag="RH058_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV3138878.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13978..14250
                     /locus_tag="RH058_003331"
     CDS             13978..14250
                     /locus_tag="RH058_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3138879.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFSKHEFDKTREYDDIRS"
     gene            14477..>14759
                     /gene="ghoS"
                     /locus_tag="RH058_003332"
     CDS             14477..>14759
                     /gene="ghoS"
                     /locus_tag="RH058_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV3138880.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFR"
BASE COUNT         3568 a         3523 c         3823 g         3845 t
ORIGIN      
        1 caaaaaaaag cgcggggtgg ggccgcgcaa aaagtatgac atttttgttt gcagcaattt
       61 ttatgaaaac gttgccatac tcaaaaggtc taaggccagg cggcgtaatg ttatttaaac
      121 aattacgcct tcagcggaat agtggttacg ctttcacgca cataacgtgg taaataccgt
      181 caataatttc agtcccttca gtttcgtgtt caaatccagg gaaatggtgg tcccaggatt
      241 gcagcgagcg taaataactt acttgcggac tgtttttatc gccgaagttt tcaccagaca
      301 gcagcatcgg gattcctggc ggatacggga taactgagtt tgccgcgatg cgtcctggca
      361 gattttcaat ggataccagt tcgacattgt tgtcgacaat cgcgttgtac gcttcacgcg
      421 gggtgatttc cgccatcggc aggccggaat aggcttcgtt caaccgtgcg ccagggttgt
      481 tttctttcag ccaggcaaac atggtgtcac ccagatcgtg aatccccatg ttcgcgtaag
      541 tgtcaggata ttgttcaaca agttccggca tcacctgcgc cagcggtgtg ttggcgtcat
      601 agtggcgttt gaaggagcaa agggtgttaa ccagagtccc ccatttccca cgggttacgc
      661 ccatagagaa caggaacata atttggaagt cagtggtgcg ggtaggcaca atgccgtggc
      721 gaccaagcca ggcagtgacc agcgccgccg gaacaccggt ttcttccagt tcaccatctt
      781 cacccattcc cggagcaagg atgctgactt taatcgggtc gagcatactc cagttatccg
      841 gaatatcttt gaagccgtgc cagctttcgc ccggatgcat tacccagcag tcctgaacgg
      901 tggtcagcag tttggttggt gcgtcagcaa agtcataggt tttgccggtt tgtgggtcgg
      961 tgacgacttc tttgttccac ggtttgaaga accagctacc gtcagcggtg aactctttat
     1021 acagccgcgc catcgcctga cgaaaatcaa ccgcttcgtc aatcacttct tgtgtcagtg
     1081 acaggccgct gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg cagatggcat
     1141 acagcgggga ggtggtggca tgcatcatgt aggcctggtt gaagcgggag aagttaatcg
     1201 ccccacgacc ttcacgtaca tgaatataag aagcctgtga cagcgcattc agcagtttgt
     1261 gggtggagtg ggtggcgaaa acggtaggac cgttgtgatc gccaggttcg ccgcgcatgg
     1321 catagtgatc ggcatagatc gggttgaaac gtgcatagcc gtaccaggct tcgtcaaagt
     1381 gcagacgatc ggaggttttt tccagcagat cctgcgcttc tttagcgtta taacacacgc
     1441 cgtcataggt gcagttggtc accacgcagt aagacggttt ttgcccagct ttgtctttag
     1501 tcagcgggct ttcactgatt ttcttctgca aggtttcagg ttgcatttcc tgcggataga
     1561 ttggcccgat aatgccgtag cggttgcggc ttggcaccat gtagactggt ttcgcgcctg
     1621 tcagcatcaa gccttgttcg atggatttat ggcaattacg gtcaacgacc acgacatcgt
     1681 tatcggtcat gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg acgactaccg
     1741 accaggagcg atcggcacca aatacgcgtg cggcatattt ttcgctttcg ccaaatgcgc
     1801 cagtatggtc aagcaaagaa ccgagggaag ttcgttcgat gcccatatcg gtgcggaaca
     1861 gattttctcc atagtagtca tggtagaaac gtccggcggg tgttttggta aaaccaacgc
     1921 cgccctggtg gcctggcgct gcccaggaat attcatggat gtcgctatat ttcatcagcg
     1981 cgctgaacag tggcggcaac agctgctggc ggtagcgggt catcgcggca acagcgcgtc
     2041 cggcgataaa gtcggcggta tcttccagaa tccaggcgaa ttcatcgaca agctccagca
     2101 gatcccggtc cattgcggcg agggcttttt cccgatcgcc caacaggaag accggcacgt
     2161 tttgttggcg ctcatgaagc ttaccgatca attgtctgac gttttgatgt tcgtccggat
     2221 gttccatttg atagctgaac atcaggcagt caatggcttc gtttgaagag agaatggcaa
     2281 aaccatcatc aaaggaggtg gatttaatca cggtaacatt ttgctggctt aaagcatctg
     2341 ccagacgctc aacggcgtta ccgacccagg tgtcttgatg gagaaactcg ctttcaacaa
     2401 ttaatacttt catcattgct tacccggtta tgaaggaatc ttcgttgcgg gcaagtatct
     2461 tccggccttt tcgggctgta aagcgcgtga aaaacaaaca tttttgaaat atttagtttt
     2521 ataaacaata cgttatgtga ttattttaaa ttattttcac tgcttattat cgacggctaa
     2581 actatttttt tggctgatac tgatatcgtc tttagccggg aaacgtctgg cggcgctgtt
     2641 ggctaagttt gcggtattgt tgcggcgaca tgccgacata tttgccgaac gtgctgtaaa
     2701 aacgactact tgaacgaaag cctgccgtca gggcaatatc gagaatactt ttatcggtat
     2761 cgctcagtaa cgcgcgaacg tggttgatgc gcatcgcggt aatgtactgt ttcatcgtca
     2821 attgcatgac ccgctggaat atccccattg catagttggc gttaagtttg acgtgctcag
     2881 ccacatcgtt gatggtcagc gcctgatcat agttttcggc aataaagccc agcatctggc
     2941 taacataaaa ttgcgcatgg cgcgagacgc tgtttttgtg tgtgcgcgag gttttattga
     3001 ccagaatcgg ttcccagcca gagaggctaa atcgcttgag catcaggcca atttcatcaa
     3061 tggcgagctg gcgaatttgc tcgttcggac tgtttaattc ctgctgccag cggcgcactt
     3121 caaacgggct aagttgctgt gtggccagtg atttgatcac catgccgtga gtgacgtggt
     3181 taatcaggtc tttatccagc ggccaggaga gaaacagatg catcggcaga ttaaaaatcg
     3241 ccatgctctg acaggttccg gtatctgtta gttggtgcgg tgtacaggcc cagaacagcg
     3301 tgatatgacc ctgattgata ttcacttttt cattgttgat caggtattcc acatcgccat
     3361 cgaaaggcac attcacttcg acctgaccat gccagtggct ggtgggcatg atatgcggtg
     3421 cgcgaaactc aatctccatc cgctggtatt ccgaatacag cgacagcggg ctgcgggtct
     3481 gtttttcgtc gctgctgcac ataaacgtat ctgtattcat ggatggctct ctttcctgga
     3541 atatcagaat tatggcagga gtgagggagg atgactgcga gtgggagcac ggttttcacc
     3601 ctcttcccag agaggcgagg ggagcgtccg agcatcatga ggccgaaaac tctgcttttc
     3661 aggtaactta ttaccataaa ctcagattta ctgctgcttc acgcaggatc tgagtttatg
     3721 ggaatgctca acctggaagc cggaggtttt ctgcagattc gcctgccatg atgaagttat
     3781 tcaagcaagc caggagatct gcatgatgac tgcacccaaa attacattta tcggcgctgg
     3841 ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc catcgcgagg cgctgaaaac
     3901 ggcgcatatt gccctgatgg acattgatcc cacccgcctg gaagagtcgc atattgtggt
     3961 gcgtaagctg atggattcag caggggccag cggcaaaatc acctgccaca cccaacagaa
     4021 agaagcctta gaggatgccg attttgtcgt ggtggcattt cagattggcg gttatgaacc
     4081 ttgcacggtg actgatttcg aggtctgtaa gcggcatggt ctggaacaaa ccattgccga
     4141 tacgttgggg ccgggcggta ttatgcgcgc gctacgtacc attccgcatc tgtggcaaat
     4201 ttgcgaggac atgacggaag tctgccccga tgccaccatg ctcaactatg ttaacccaat
     4261 ggcgatgaat acctgggcga tgtatgcccg ctatccgcat atcaaacagg tcgggctgtg
     4321 ccattcggtg cagggaacgg cggaagagct ggcgcgtgac ctcaatatcg acccagctac
     4381 gctgcgttac cgttgcgcag gtatcaacca tatggcgttt tacctggagc tggagcgcaa
     4441 aaccgccgac ggcagttatg tgaatctcta cccggaactg ctggcggctt atgaagcagg
     4501 gcaggcaccg aagccgaata ttcatggtaa tactcgctgc cagaatattg tgcgctacga
     4561 aatgttcaaa aagctgggct acttcgtcac ggaatcgtca gaacattttg ccgaatatac
     4621 gccgtggttt attaagccag gtcgtgagga tttgattgag cgttataaag taccgctgga
     4681 tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg cataaagagc tggaggagta
     4741 taaaaacgcc tcccggattg atattaaacc gtcacgggaa tatgccagca caatcatgaa
     4801 cgctatctgg actggcgagc cgagtgtgat ttacggcaac gtccgtaacg atggtttgat
     4861 tgataacctg ccacaaggat gttgcgtgga agtagcctgt ctggttgatg ctaatggcat
     4921 tcagccaacc aaagtcggta cgctaccttc gcatctggcc gccctgatgc aaaccaacat
     4981 caacgtacag acgctgctga ccgaagctat tcttacggaa aatcgcgacc gtgtttacca
     5041 cgccgcgatg atggacccgc atactgccgc cgtgctgggc attgacgaaa tatatgctct
     5101 tgttgacgac ctgattgccg ctcacggcga ctggctgcca ggctggttgc accgttaaaa
     5161 cgcgactaaa cgctactgcg ccgggggatt tattccggcg cacacctctg acgataccaa
     5221 taacagaagg cgggcgttgg taacagcgac ccgataccct atgagcattt caatgactac
     5281 aaaactcagt tatggatttg gagcgttcgg gaaggatttt gcgatcggca ttgtgtatat
     5341 gtacctcatg tattactaca ccgatgtcgt tgggctgtct gtgggtttgg tcggtacttt
     5401 gtttctggtg gcgaggatct gggatgctat taacgatccg attatgggat ggattgtaaa
     5461 tgctacgcga tcgcgatggg gtaagttcaa accctggatc ctgatcggta cgttggcaaa
     5521 ctctgtaatc ttatttctcc tctttagtgc gcatctgttt gaaggtacta ctcagattgt
     5581 ctttgtttgc gtgacctaca tcctctgggg catgacttac accattatgg atattccctt
     5641 ctggtcgctg gttccaacca tcacgctcga taaacgtgag cgcgaacaac tggttcctta
     5701 tccgcgtttt tttgccagtc tggcaggctt tgttacggca ggtgtgacgc taccatttgt
     5761 taattatgtc ggcggtggcg atcggggatt tggctttcag atgttcactc tggtactaat
     5821 cgcctttttt attgtttcaa ccatcatcac tctgcgcaat gtgcatgaag tcttttcgtc
     5881 agacaatcaa ccgtctgctg aaggaagcca tctgacactt aaagccatcg ttgcgctaat
     5941 ttataaaaac gatcagcttt catgcctctt gggtatggct cttgcttata atgtagccag
     6001 caacattatt accggatttg ctatctatta tttctcatat gttatcggtg atgcggattt
     6061 gttcccctat tatctgtcgt atgcgggagc tgctaacctg gtgacgttag tattcttccc
     6121 acgcttagtt aaatcattat cccgacgcat tttatgggcc ggagcatcta ttcttccggt
     6181 gttaagctgt ggtgttctcc tgttaatggc attaatgagc tatcacaacg tcgtcctcat
     6241 tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt ttctgggtat tacaggtcat
     6301 catggtggca gataccgttg attacggtga atataaactg cacgtacgct gtgaaagtat
     6361 cgcttactcc gtgcagacta tggtggtgaa gggcggttca gcctttgcgg cttttttcat
     6421 tgcggttgtg ttagggatga ttggctatgt accgaatgtt gaacagtcta cgcaagccct
     6481 attaggtatg cagtttatta tgattgctct accaactctg tttttcatgg taacgctgat
     6541 tctctacttc cgtttctatc gcctcaatgg cgacacgctg cgcaggatcc agattcatct
     6601 gctggataaa tatcgcaaag taccgcccga gcctgttcat gctgatattc cggtcggtgc
     6661 agtgagtgat gtgaaagcct gacgtggaca atagctaacg aaaatggcct gatgcgatat
     6721 gtttatcagg ccacgtctta atgtaatgct ttggatttgc aaattttgta gaccggataa
     6781 ggaattcacg ccgcatccgg catcaacaaa gcgcacgttg ttatccggtt atcaagccaa
     6841 agcgccgtag ctggcggcaa tgaaagcgag cgaggaatat caccgtcgtt aatgcgccaa
     6901 gcagcgcgca gaacatatcg gattgcgtgt cccactggtc gccctgggtg ccgagaaaat
     6961 catccgctcc ctggcccatt gccagcgccg cccaccactc gattaattca tacatcgcgc
     7021 ttatcgccag cgccacgcag cagaccagga acgccaccat tttacgtccg cgcacgtaca
     7081 tcccgcgcac gagaatttct cgtgccacca gtgcaggcac cagcccctgg aaaaaatgcc
     7141 ccagcttgtc atacggatta cggctcaacc ccagccattc ctgcacctca aaaccaacgg
     7201 ggactttcgc gtaggtgtat tgcccgccga ccatcaggat gatggcgtga aagaaaatga
     7261 gcgtatagag cagcggcgtt aacggataac gtttagcggt ggcaagcagt agcggcacga
     7321 caataatcac cggtgtcact tccatcagcc aggtgagttt gtcatgggcc gaaatgccgg
     7381 tataaatcag gattaacgtt agcgccagtg cgccggtgtt aagaattaac ggcttgagtg
     7441 tgcgggtcat ggtgagttcg caaatcaggg aaaatactga ctattcacca gcaagcgtga
     7501 aattacaatc gcaaaagagg tggcccaggg ggatcacctg gcagcatgct gccaggcgct
     7561 gggccgaaga ggttacttag tgcagttcgc gcactgtttg ttgacgattt gctggaagaa
     7621 gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa tcttctactt cgattttcca
     7681 gatagcttcc atacccagct ccggataagc gacgcattcc agatgcttga tgctctgctg
     7741 cgccagtacc gccgccggac cgccgatgct accgaggtag aagccgccgt gtttatgaca
     7801 cgcgtcggta acctgctgac tgcggttacc tttcgccagc atgatcatgc tgccgccgtg
     7861 ggattgcagc agatccacgt aggagtccat acggcctgcg gtggttgggc caagtgaacc
     7921 tgatggataa ccggcagggg ttttcgccgg acccgcgtag tagatcgggt gatctttgat
     7981 gtactgcgga agttctttac cggcgtcaat cagctctttc agcttggcgt gtgcaatatc
     8041 tcggcccacg ataatggtgc cggtgagcga caaacgagtg gataccgggt attgcgaaag
     8101 ctgggcgagg atctctttca tcgggcggtt aaggtcaact ttcaccgctt cgccttcacc
     8161 ggcctggcgc agttcttgtg gaatgtactg gcctgggttg tgttccagtt tttcgatcca
     8221 gataccttcg cggttgattt tcgctttaat gttacggtca gcggagcagg agacgcccat
     8281 gccgaccggg caggatgcgc cgtgacgtgg cagacggata acgcgaatgt cgtgcgcgaa
     8341 gtatttaccg ccaaactgcg cgccaagacc gagtttctgg gcctcttcca gcagttcctg
     8401 ttccagctgg acatcgcgga acgcctgacc atgttcgttc ccttccgtcg gcagttcatc
     8461 gtaatagtga acgcttgcta acttgacggt tttcaggttg gtttccgcag acgtaccgcc
     8521 aatcacaaac gcgatatggt acggcgggca ggctgcagta ccgagggtac gcattttctc
     8581 gacgaggaag tttttcagtt tgccgggagt cagcagggct ttggtttcct ggtagagata
     8641 cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg aatttgtact catcgccatc
     8701 taccgcgtac aggtcgattt gcgcaggcag gttagtgccg gtgttgacct ctttgtacat
     8761 gtccagcgcc gcattctgtg aatagcgcag gttatcttcg atataggtgt tatagacgcc
     8821 tttcgacagc gcttcttcat caccgccgcc ggtccacacg cgctggcctt ttttaccgac
     8881 gatgatcgcg gtgccggtat cctggcaggt cggcagcacg cctttggcgg cgatttcgga
     8941 gtttcttaag aattgcagcg ccacgtactt gtcgttttcg ctggcttctg gatcgtgaag
     9001 aatagccgca acctgtttct ggtgtgccgg gcggagcata aaagaagcgt cgtgaaaggc
     9061 ttgctgcgcc agcagggtca gggcttctgg ttccactttc aggatggttt cgccgtcgaa
     9121 gtcggcaacg ctaacgtaat cggaagtgag tagatagtat tcggtattgt ctttccccat
     9181 cgggaaaggt gcctggtaga taaagggttt gtttgacata gcttccagcc tgtaactctg
     9241 tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc ggcacgcaaa gtgcatttat
     9301 aagaacccgt acatcgcggc gaagatccag ccgaagacgc acgatacgct cacaccaatc
     9361 aaccccggca gaataaagct gtggttgatg acgaagcgac cgatgtgggt ggtgccggaa
     9421 cggtcaaact gaatcgctgc cagatcgctc ggataagtcg gcaggatgta ataaccgtag
     9481 caagccggtg ctgaagccac gatgtatgcc ggatcaacgc cgatcgccag cgcgaccgga
     9541 acaatcgccg ccagcgccgc agcctgagag tttacaaact tggaaaccag cagcagaaca
     9601 atggcatagg cccacggata ctctttcacc atttcaccca gtacgccctg aatttcagac
     9661 atatgcgcac cgaacatggt ttctgccatc catgcgatac cgtacaccgc cacgatggcg
     9721 atcataccgg aacggaagac ttcgtttttt gagatagacg cgggattggt tttggtcagg
     9781 ataataatca gcgccccggt cagcagcata aacatctgaa taaccagtac catcgacagc
     9841 ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac caagaagggc gactacagcg
     9901 attgcaccga ggaaaatcca cattgccagc cagttgcttt tcggcagttt tttatcgagc
     9961 agcgtcgcgg tatcaccgta aacatactca cggttttccg gtacggagat gaatttctgg
    10021 aactcttcgt ctttatccag atctttaccg cggaaccagc tgaagatacc gatcgccagg
    10081 ataccgatta acgtcgatgg aatggtgatt gccagcaggt cgaggaactc aagatggcga
    10141 ccatcaaagg tgacattacc cagcattgca accagagaca cgaccgcaac cgacaccgga
    10201 ctggcgataa tccccatctg tgcaccgata gaacttgccg ccatcggacg ttccggacgg
    10261 atgttgttct taatggcgac gtcgtagatg atcggcagaa tggtgtaaac cacatgaccc
    10321 gtaccgcaaa gaatggtcag tgtacaggtc acaaacggcg cgacaattga gacatatttc
    10381 gggttgcggc gcagcagctt ctcggcaatt tgcagcatga catcaagacc gcccgaagct
    10441 tgcaaggtcg ccgatgccgc caccaccgca atgataacca gcatgacatc aaccggtggt
    10501 ttacctggct gaaggtggaa gacgaagacc agaatgacca gaccgatacc gcctaataaa
    10561 cccagcgcga taccaccctt tctggcacca taaaacagac atatcagtat tatgataagt
    10621 tggatagtaa ataacatgtg tgaaccctcg cgataatcct atttaaattt ttgctgaata
    10681 gatcacagtc acgttctgtt ttgtatgaac tgttttcaga gcctgactaa atatccgttt
    10741 ggtcgttacc ggctttagca aatacctcac agtaaatatt ggctgattaa agcagcggca
    10801 aatttatgca ctgtgataag cggctttttc agagagaaaa actctctggc gatgggttta
    10861 tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt atcttattga tatttatcgg
    10921 tctatttttt gttctcaggg cagatatcat taggtttaaa gattaatctg atctacccat
    10981 ttgtgggtaa aaatacacat aatgcgggtg acataatagt taattaactt ttgttatcgt
    11041 tttgaaatta aaaacaccgt tcacctgaag agatattaat ttttagcgat gatggaggga
    11101 taattatatt tgatctggca caagttttac tgatgaagga tgtaacttgt gccaggggta
    11161 ttttgcatta cggtaataat tattacttac agataaccag cgatccgtta ttggcaatat
    11221 tgtttcagta gtgagtagtg ttctgcctga atacggtaac ggtaaaccgg acgccccgtg
    11281 acgccataat ggatactggt gaacaagatg tggcagttga ccagccagat gaggtattta
    11341 cggcaggaaa cacgcgagat gttaacctcg ttagccagct cgtcggttga aaactcatag
    11401 tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg tctgcggcgt taagcctttt
    11461 ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt ggattagctg atcaagctcg
    11521 gcctggtcat aatactgatg tttttccagc gccattttct tttgccgcca gccggtgagc
    11581 gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt aatccacgac accgtaatgc
    11641 agcgaatctt taatggttgc cgcatcggct gcggaggaga tgacaatcac atcacttttg
    11701 caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt tctctttttg catatagata
    11761 tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga taatctcttt ggctttctcc
    11821 agcgtcgagg ctgttccaca gcattgaaag cctgggattt gtgctacgta tcggcgattc
    11881 agctccgcga ccattgcgtc gtcatcgata attaatacat tgatcatctg ttcgacctct
    11941 ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat cccgggttcc gattccacgg
    12001 cgatgctgcc gccgagattt tctacctgtt gtttgacaag tgctaaaccg acgcctcgct
    12061 cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc gattttgtcg ggtgcgatcc
    12121 ccggtccatc atcgttaact tcgcagtgca gccagccgtg acggtagtgc aatgttacgc
    12181 taatttcgcc accgggttcc ggccctaatg cttccagcgc gttttctatc agatttccca
    12241 atgtggtaat cagcgtcgcg acctggtcct cactgccgct gtctggcagc tggctttcac
    12301 tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcgatt aatcttgctg attaaaaaac
    12361 cagcgataac cggagatttg atcttaccca gcagagagcc aatctcttcc tgatagttat
    12421 tggctgtttt gagaatgtaa tcttccaact gcttataact cttcagatgc aataatccga
    12481 gaatcacatg caatttattc ataaattcgt gggatcgttc acgaagtgca tcagcatagt
    12541 tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc agttttgtcc ctgaaggttg
    12601 aaatggcacc gatgataacg ccattactgc gcaccggaac ggtgttgatc agtaatagcc
    12661 gatctttaat cgtaatctct tcgtcgcggc gcggcgtacc gtcgcgtaac acttccgaga
    12721 catctaccac ctgtgaccat gagtggctga gcgtcgacag tttctcatcg tcctgcgact
    12781 tacggtaatt cagcaattct tgtgcggcat cgttgatcag cgtgacctcg ccgcgatcgt
    12841 ccacggcaac gacgccttct ttgatagact gcaacatggc ctggcgctgc tcaaacagcg
    12901 tggagatttc gtagggttcc aggccgaaaa ggattttttt cagtacctta accagaatgc
    12961 aggtgccaat cagtccgacc agcatgccaa ataataccga ccagataatg ctccagcgac
    13021 tgtcattgat ttgttgggtc acgcggctta actcaaggcc gatcgccacc acgccaattt
    13081 gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa agcctgcgcc agaaaaccgc
    13141 gattgatagc gacattttct ttgccattca gcgccttaag gatgtcatca cctttaaatg
    13201 gctgaccaat acgctgggct tcaggatgcg agtagcgaag actttgcata tcggtaacga
    13261 caataaacag cagatcgttg cgtttgcgca cggcttccgc gatggcctgg attccgctct
    13321 cctgcggttt tttctgcaag ccctgacgga tttccggcga gtcggcgagg gtacgcgcca
    13381 ctgccagtgc cttgttggct agcccgtctc gcgtcatatc actgatttgc gagaagtaaa
    13441 tcagatgcac caccaatagc accgagaaca gtaccgcact gaccattaag atcactgtgg
    13501 tactcaattt catcggacgt ttgcgtaaca tgcggtaggg caatgaatgt ctcatcagct
    13561 tccttgtgtg acaaatttct taagcattat ctctgatgag gcgggtaatt caaagggagt
    13621 aagaatgatt ggctatatag gggaagagac tctggcaacg gaaactgcca gtgctgtatg
    13681 aagattccag ggctatgctt atagcgataa tcatactgat gagagaggga aagtcatgga
    13741 tcaggcgcta ctggacgagg gttatcgctg ttataccggc gaaaagatcg atgtctattt
    13801 caacactgcg atatgtcagc attctggcaa ttgcgtacgt ggcaacggca agttatttaa
    13861 tctcaaacga aagccgtgga tcatgccgga tgaagtcgac gtcgtcactg tggttaaagt
    13921 gattgatacg tgcccgagcg gcgcgctgaa ataccgtcat aaataagcga gggtaaaatg
    13981 gaaatacgcg aaggccacaa taaattttac attaatgacg aacagggcaa gcaaatcgct
    14041 gaaattgtct ttgtgccgac cggagagaat ttagcgatta tcgaacatac cgatgtcgat
    14101 gaaagcctga aagggcaggg gattggtaaa cagctggttg cgaaagtcgt ggaaaaaatg
    14161 cgtcgggaaa aacgaaaaat tatcccacta tgcccatttt cgaaacacga atttgataaa
    14221 acgcgggagt atgatgatat tcgcagttga tgggagagta cagagtcacg atatttttca
    14281 tgctctccgc ggagtgatgc aggagagcat ctgaagggta gggggatgca caaagaatgg
    14341 gcagagagag cgtttttttg tcccaagtca tcccctttac tgagcaaaaa aaagaatatc
    14401 tcctatatga gaatcatcaa tcggggttaa taagttttgc gtccccagag cgtttaatat
    14461 tgataggagt catattatgg aaggtaaaaa caagttcaat acttatgttg tttcttttga
    14521 ttatccatca tcttattcct cagtgttctt aaggttaaga tcattgatgt atgatatgaa
    14581 tttctcctct atcgtggctg atgaatatgg gataccacga caattgaatg aaaactcctt
    14641 cgcaataacg acatcgttag ccgcaagtga aatcgaagat ttaatcaggc ttaaatgctt
    14701 agacttaccg gatattgatt ttgacctcaa cattatgaca gttgatgact atttccgtc
//
LOCUS       DAPHDX010000107        14630 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPHDX010000107 DAPHDX010000000
VERSION     DAPHDX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.108"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..441
                     /locus_tag="RH058_003333"
     CDS             <1..441
                     /locus_tag="RH058_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV3138881.1"
                     /translation="AGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYE
                     FICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMR
                     IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            597..2132
                     /gene="gadC"
                     /locus_tag="RH058_003334"
     CDS             597..2132
                     /gene="gadC"
                     /locus_tag="RH058_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV3138882.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2263..3582
                     /locus_tag="RH058_003335"
     CDS             2263..3582
                     /locus_tag="RH058_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV3138883.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3954..5336
                     /gene="dosC"
                     /locus_tag="RH058_003336"
     CDS             3954..5336
                     /gene="dosC"
                     /locus_tag="RH058_003336"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV3138884.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPFIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5361..7760
                     /gene="dosP"
                     /locus_tag="RH058_003337"
                     /gene_synonym="pdeO"
     CDS             5361..7760
                     /gene="dosP"
                     /locus_tag="RH058_003337"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV3138885.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8018..8599
                     /gene="ddpX"
                     /locus_tag="RH058_003338"
     CDS             8018..8599
                     /gene="ddpX"
                     /locus_tag="RH058_003338"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV3138886.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8613..10163
                     /locus_tag="RH058_003339"
     CDS             8613..10163
                     /locus_tag="RH058_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3138887.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10165..11187
                     /locus_tag="RH058_003340"
     CDS             10165..11187
                     /locus_tag="RH058_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3138888.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLGGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11184..12080
                     /locus_tag="RH058_003341"
     CDS             11184..12080
                     /locus_tag="RH058_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV3138889.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMNFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12077..13063
                     /locus_tag="RH058_003342"
     CDS             12077..13063
                     /locus_tag="RH058_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3138890.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRSEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDRVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13056..13982
                     /locus_tag="RH058_003343"
     CDS             13056..13982
                     /locus_tag="RH058_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3138891.1"
                     /translation="MSNTLLTLCDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14038..14469)
                     /gene="osmC"
                     /locus_tag="RH058_003344"
     CDS             complement(14038..14469)
                     /gene="osmC"
                     /locus_tag="RH058_003344"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV3138892.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVLLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3594 a         3643 c         3713 g         3680 t
ORIGIN      
        1 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctacacc
       61 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaactg
      121 gggccgtatg agttcatctg tacgggtcgc ccggacgaag gcatcccggc ggtttgcttc
      181 aaactgaaag atggtgaaga tccgggatac accctgtacg acctctctga acgtctgcgt
      241 ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga catcgtggtg
      301 atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt gctggaagac
      361 tataaagcct ccctgaaata tctcagcgat cacccgaaac tgcagggtat tgcccagcag
      421 aacagcttta aacatacctg ataacgttta acggtaacgg tgtcccgaaa cgaacccgtt
      481 tcgggacaat ttccaaagtc tgttcactgg cattagcaac ggaaaatatt gttctgaata
      541 cgcttcagaa caaaacaggt gcggttccga caggaatacc gttttagggg gataatatgg
      601 ctacatcagt acagacaggt aaagctaagc agctcacatt actcggattc tttgccataa
      661 cggcatcgat ggtaatggct gtttatgaat accctacctt cgcaacatcg ggcttttcat
      721 tagtcttctt cctgctatta ggcgggattt tatggtttat tcccgtggga ctttgtgctg
      781 cggaaatggc caccgtcgac ggctgggaag aaggtggtgt cttcgcctgg gtatcaaata
      841 ctctggggcc gagatgggga tttgcagcga tctcatttgg ctatctgcaa atcgccattg
      901 gttttattcc gatgctctat ttcgtgttag gggcactctc ctacatcctg aaatggccag
      961 cgctgaatga agaccccatt accaaaacta ttgcagcgct catcattctt tgggcgctgg
     1021 cattaacgca gtttggtggc acgaaataca cggcgcgaat tgctaaagtt ggcttcttcg
     1081 ccggtatcct gttacctgca tttattttga tcgcattagc ggctatttat ctgcactccg
     1141 gtgcccccgt tgctatcgaa atggattcga agaccttctt ccctgacttc tctaaagtgg
     1201 gcaccctggt tgtatttgtt gccttcattt tgagttatat gggcgtagaa gcttccgcaa
     1261 cccacgtcaa tgaaatgagt aacccagggc gcgactatcc actggctatg ttactgctga
     1321 tggtggcggc aatctgctta agctctgttg gcggtttatc tattgcgatg gtcattccgg
     1381 gtaatgaaat caacctctcc gcaggtgtaa tgcaaacctt taccgttctg atgtcccatg
     1441 tggcaccgga aattgagtgg acggttcgcg tgatctccgc actgctgttg ctgggtgttc
     1501 tggcggaaat cgcctcctgg attgttggtc cttctcgcgg gatgtatgtc acagcgcaga
     1561 aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa tggcgtaccg gtaacgctgg
     1621 tcatttcgca gctggtgatt acttctatcg cgttgatcat cctcaccaat accggtggcg
     1681 gtaacaacat gtccttcctg atcgctctgg cgctgacggt ggtgatttat ctgtgtgctt
     1741 atttcatgct gtttattggc tacattgtgt tggttcttaa acatcctgac ttaaaacgca
     1801 catttaatat ccctggtggt aaaggggtga aactggtcgt ggcaattgtc ggtctgctga
     1861 cttcaattat ggcgtttatt gtttccttcc tgccgccgga taacatccag ggtgattcta
     1921 ccgatatgta tgttgaatta ctggttgtta gtttcctggt ggtacttgcc ctgcccttta
     1981 ttctctatgc tgttcatgat cgtaaaggca aagcgaatac cggcgtcact ctggagccaa
     2041 tcaacagtca gaacgcacca aaaggtcact tcttcctgca cccgcgtgca cgttcaccac
     2101 actatattgt gatgaatgac aagaaacact aaccatgtct gaatggttat aagacaaggg
     2161 agcgataagt catcgctccc tttttcgtgc ttgctgtcgt attgacttaa ccgggataag
     2221 tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat ctgctcccga
     2281 aacaagaaat taacgattag aagaccagcg atactagttg cactggcact tttactgtgt
     2341 agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc aaagccacca
     2401 gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac ggtttctcgg
     2461 ctcgactggc caccggtttc ctcggttaac attagtaacc ccaccagccg ggcccgtgta
     2521 caacaacagg cgatgatcga caaactggat catctgcaac gtctcggcat aaacacggtc
     2581 tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc
     2641 gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca atttatgctc
     2701 gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg
     2761 gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca acaaccggcg
     2821 agcgtctatg tgcaacaccg tgactggatc agaacgtctg gcgatcgctt tgtcctcgac
     2881 ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgctgaagt ggtttcccgc
     2941 tatccggtag atggcgtgca gtttgacgac tatttctata ctgaatcacc gggttcacgg
     3001 ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat ttgcgtcaaa agcagactgg
     3061 cggcgcaaca atactcagca gttaattgca aaggtatcac acaccattaa aagcattaag
     3121 ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc gtaaccgatc acacgatccg
     3181 ctcggttccg atacccgagg cgcggcagcc tatgacgaat cctacgctga cacccgtcga
     3241 tgggtggaac aaggattgct ggattacatt gctccccaaa tttactggcc gttctcacgg
     3301 agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa accgaccagg
     3361 acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccttcaaa gatagagcca
     3421 gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc agctcgatct taacgatgcg
     3481 gtaccagaaa ttagcggcac catcttgttc cgtgaggact atctgaataa accgcagact
     3541 caacaagcgg tcagctatct gcaaagtcgt tggggcagtt aaactcccat gcttactttg
     3601 taacaagccg gtgctttacc caccggcgtg ttacaccttg tgaaatatcc cactagtaat
     3661 catgcttaca taagtcaaat taaccacgca attaaccaca cttagttaaa cagctataaa
     3721 tccctaataa aaatgtatga atatgaaaaa ccatacgata ttcgctgtca ccgaaaaata
     3781 attacgatgg gctatactct tctgaagaac agtagcgtag attaaaactg tcctaagtcg
     3841 cttatttctt ttcagtatat cttcatattt caggagaata tggtgcagtt agtcgatctc
     3901 gcacgttgcg ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga
     3961 tgtattttaa aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctttca
     4021 ttcgtgctaa agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt
     4081 atcgaattgt ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc
     4141 ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg
     4201 atgtcgaaag gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa
     4261 ttccggtaga aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa
     4321 tcttctcttc ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca
     4381 gtattgatat cgctatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct
     4441 caaaggaaga tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag
     4501 aacggcaaat agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg
     4561 attctgattt aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc
     4621 ataaaggtcg acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc
     4681 aggatttcga cggtattttc aatcaaacca tgcgtaacac caggaatttg aataacagaa
     4741 gtttgcgggt gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc
     4801 tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat
     4861 tacttaaccg ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga
     4921 ccggtacacc actgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata
     4981 cgtggggcca taacactggc gatgaaattc tgcgtaaagt ctctcaggcc ttttatgaca
     5041 acgtccgcag tagtgattat gtttttcgct acgggggcga tgaatttatc attgttttga
     5101 ctgaagcttc tgaaaacgaa acgttacgta ccgcagaacg tattcgcagt cgggtggaga
     5161 aaaccaaact gaaagccgca aacggcgagg atattgccct ctcactttcc atcggtgccg
     5221 ccatgtttaa tggtcatcct gactatgagc gcctcattca aatagccgat gaagctctgt
     5281 atatcgccaa aagacgaggt agaaaccgtg ttgaactctg gaaagccagt ctttagatgc
     5341 gccaggatgc agaggtaatc atgaagctaa ccgatgcgga taatgccgcc gatggcattt
     5401 ttttccccgc ccttgagcaa aatatgatgg gtgcggtgtt aattaacgaa aatgatgaag
     5461 tgatgttttt caaccccgcc gcagagaagc tctgggggta caaacgtgaa gaagtcattg
     5521 gcaataacat tgatatgctg attccgcggg atttgcgtcc tgcgcatcct gaatacattc
     5581 gtcataaccg tgaaggcggt aaagcgcgtg tcgaggggat gagtcgggag ctgcagctgg
     5641 agaaaaaaga cggcagtaaa atctggaccc gttttgcgct atcgaaagtg agcgccgagg
     5701 ggaaagttta ttacctggcg ctggtacggg atgccagcgt agaaatggcg caaaaagaac
     5761 agactcgaca attgattatt gccgttgacc atctcgaccg accggtgatt gtcctcgatc
     5821 cggaacgcca tattgtgcag tgcaatcgcg catttaccga aatgtttggt tactgcatta
     5881 gcgaagccag cggtatgcag cccgatacac tcctgaacat tcctgaattc cctgccgata
     5941 accgcattcg tttacaacag ttgctatgga aaaccgcccg cgatcaggat gaatttctgc
     6001 tgttgacgcg caccggtgaa aaaatctgga ttaaagcctc tatcagcccg gtttatgacg
     6061 tgctcgcgca tctgcagaac ctggtaatga ccttctcgga tatcaccgaa gaacggcaaa
     6121 tccgccagct tgaaggcaat attctcgccg ccatgtgcag cagcccgcca tttcatgaaa
     6181 tgggggaaat catttgtcgt aacatcgaat ctgttctcaa cgaatcgcat gtttcgctgt
     6241 tcgcactgcg caacgggatg ccgatacact gggcgtcatc ttcccacggt gcagaagttc
     6301 aaaatgcgca aagctggtca gcgaccattc gtcagcgtga tggcgcgcct gcggggatcc
     6361 tgcaaattaa aacctcgtca ggagcagaaa ccagcgcctt tatcgaacgc gtggcagata
     6421 tcagtcagca tatggccgcg ctggcgctgg aacaggaaaa aagccgccag catattgaac
     6481 aactcatcca atttgatccg atgaccggtc tgccaaatcg caataacctg cacaattacc
     6541 tcgatgacct ggtcgacaaa gccgtctctc cggtggtgta tctcatcggt gttgaccata
     6601 ttcaggatgt gattgatagc cttggctatg cgtgggccga tcaggcattg ctggaagtgg
     6661 tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc gaaggtacgc
     6721 agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat tacccaaatc gccgatgagc
     6781 tacggaatgt ggtcagcaag ccgataatga ttgacgataa acccttcccg cttaccttga
     6841 gtattggcat cagctacgac gtgggtaaaa accgcgatta cctgctctcc actgctcaca
     6901 atgcaatgga ttatattcgc aagaatggcg gtaacggctg gcagttcttc agcccggcga
     6961 tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa gcgattagca
     7021 ataaccaact gaaacttgtt taccagccgc aaatcttcgc agaaacgggt gaactgtacg
     7081 gcatcgaagc ccttgcgcgc tggcacgatc cccagcatgg tcatgtgccc ccttcacggt
     7141 ttattcctct cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg
     7201 aagcttgccg tcagttagca gaatggcgta gccagaatat tcatatcccg gcgctttccg
     7261 ttaacttgtc ggcactgcac tttcgcagta atcaactgcc taatcaggtg tctgatgcaa
     7321 tgcacgcctg gggtattgac ggccaccagc tgacggtaga aatcacggaa agcatgatga
     7381 tggaacacga taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggct
     7441 tatcggtaga tgattttggt acgggctttt ccggattatc ccgcttagtc agtcttccgg
     7501 taacggaaat caaaattgac aaaagttttg tcgatcgttg tctgaccgaa aaacgcatcc
     7561 ttgccttact tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg
     7621 aaggcgtcga aaccaaagag caatttgaga tgctacgcaa gatccactgt cgcgttattc
     7681 agggatattt cttttcccgc cccctacccg ccgaagaaat tccaggctgg atgagcagcg
     7741 tgttaccgct gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcatttaacg
     7801 tgacactgtc acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac
     7861 ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat
     7921 ataactttaa gccagcattc tggcgcttat cccggcatgg catgagattt gcataagcgg
     7981 aaagcgcagc aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt
     8041 tgatttagcc gtaatcttcc ctgatctgga gatcgaattg aaatacgcct gcgctgataa
     8101 catcacaggt aaagctattt atcagcaagc gcgttgtctg ctacacaagg atgcgattac
     8161 cgcgctggcg aaaagtatca gtatcgccca gctgtcaggc ttacaactgg tgatttacga
     8221 tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata
     8281 tgttgttgat gtgacggtcg gttctaatca cagccgtggc acggcgatcg acctgacgct
     8341 tcgtgatgag cacgggaaca tcctcgatat gggcgcaggg ttcgatgaaa tgcacgagcg
     8401 ctcccatgcc tatcaccctt ccgtcccgcc cgccgctcag cgcaatcggc tgttgctgaa
     8461 tgcgataatg actggtggcg gttttgtcgg tatctccagc gaatggtggc acttcgaatt
     8521 accccaggca gcgagttacc ctctgctcgc tgatcaattc acctgtttta tatcccctgg
     8581 cacacaacac gtcagttaat caggaaactg tcatgaagag atcgataccg tttcgtccca
     8641 cattgctcgc gctcgtcctt gccacaaatt tcccggttgc gcacgccgcc gtaccgaaag
     8701 atatgctggt gattggtaag gccgccgatc cacaaaccct cgacccggcg gtaacaatag
     8761 ataataacga ctggacggtg acctacccgt cttatcagcg gctggttcag tacaaaacgg
     8821 acggtgataa aggctcaacc gacgttgaag gcgatctggc aagtagctgg aaagcgtctg
     8881 acgatcaaaa agagtggacg ttcaccctga aggacaatgc taaatttgcc gatggcacac
     8941 cggtcactgc cgaagcagta aaactctctt ttgagcggct actaaaaatc ggccaggggc
     9001 cagcagaagc atttcccaaa gatttaaaga ttgatgctcc cgacgaacat acggtgaagt
     9061 ttacccttag ccagccattc gcaccgttcc tctacacgct ggcgaatgac ggtgcatcca
     9121 ttatcaatcc ggcggtgtta aaggaacatg cggcggatga tgcccgcggc ttcctcgcgc
     9181 aaaataccgc cggttccgga ccatttatgc tgaaaagctg gcaaaaaggt cagcaattag
     9241 ttctggtgcc aaatccgcat taccccggca ataaaccgaa ctttaagcga gtatcggtaa
     9301 aaattatcgg tgaaagtgcc tcccgtcgcc tgcagctctc ccgtggcgac attgacattg
     9361 ccgatgcgct gccggtggat caactcaacg ccctgaagca ggaaaataaa gtcaatgtgg
     9421 cagagtatcc gtcactgcgc gttacctatc tgtatctgaa taacagcaaa gcgcctctta
     9481 atcaggcgga tctgcgtcgg gccatttcct ggtctaccga ttatcagggc atggttaacg
     9541 gcattctgag tggtaacgga aaacagatgc gcggcccgat tccggaaggc atgtggggct
     9601 acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa agccaaagcc gaatgggata
     9661 aagtgacgag caaacccacc agcctgacgt ttctctactc cgataacgat ccgaactggg
     9721 agcctattgc tttggcgaca caatccagtc tcaacaagct gggtatcaat gtgaagctgg
     9781 aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa aggtgattac gacattgcga
     9841 ttggcaactg gagtccggat tttgccgacc cgtatatgtt tatgaattac tggtttgagt
     9901 cagacaaaaa aggtctgccg ggtaaccgct cgttctatga aaacagtgag gtcgataagt
     9961 tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg tacccgggac taccagcagg
    10021 cacagaaaat cgtcattgat gacgctgctt atgtgtacct gttccagaaa aactaccaac
    10081 tggcgatgaa caaagaggtg aaaggctttg tgttcaatcc catgctggaa caggtcttca
    10141 atatcaatac catgagtaaa taacgtgacc ttctggagta ttttacgcca acgctgctgg
    10201 gggctggtgc tcgtggtggc gggcgtctgc gtgattacgt ttattatctc gcatctgatc
    10261 cctggcgatc cggcgcggtt actggggggt gaccgcgcca gcgatgctat cgtggaaaat
    10321 attcgccagc aactgggact ggaccagcca ctgtatgtac agttttaccg ctacgtcagc
    10381 gacctgtttc atggtgacct gggaacatcc attcgtaccg ggcgtccggt gctggaagag
    10441 ttgcgtatat ttttcccggc gacgctggaa ctggcttttt gtgccctgct gctggcactc
    10501 ctgattggca tcccgctggg catactctct gcagtctggc gaaatcgctg gctggatcat
    10561 ctggtgcgaa taatggccat taccggaatc tccacacctg cgttctggct tggactgggc
    10621 gtcattgtgc tgttttatgg tcatctgcaa attcttcccg gcggcggaag gcttgatgac
    10681 tggctggatc caccaacgca cgttaccggc ttttatctgc tcgatgcgct gcttgaaggc
    10741 aacggtgaag tcttcttcaa tgcgttgcaa catctcatct taccggcatt aacgctggcg
    10801 ttcgttcacc tgggaattgt cgctcgccag atccgctcag cgatgctgga acaattgagt
    10861 gaagactaca ttcgtaccgc ccgggccagc ggcttgcccg gctggtatat cgttttatgt
    10921 tatgcgctac ccaatgcgtt gatcccatcg attaccgtat tgggtttggc gctgggcgat
    10981 ttgttgtatg gcgcagtgct caccgaaacc gtttttgcct ggcccggaat gggtgcatgg
    11041 gtagtaacat caatacaggc gctcgacttc ccggcagtga tgggctttgc cgtcgtggtt
    11101 tcatttgctt atgtgctggt caacctggtg gtggatttgc tctatttgtg gattgatccg
    11161 cgtatcggac gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc gtacgcccac
    11221 aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc agtccgctca
    11281 ccgtgaccgg cgcagtcatc attgtattaa tgctactgat gatgaatttt tcaccgtggc
    11341 tggcgacgca tgatcccaac gccattgatt taaccgcccg ccttttgccg ccttctgcgg
    11401 cgcactggtt tggcaccgat gaagtgggac gcgatctgtt cagccgcgta ctggtcggca
    11461 gtcagcaatc aattctcgcc ggattagtcg tggttgccat tgcgggtatg attggttcgc
    11521 tactcggatg tctatccggt gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca
    11581 tggacattat gctgtcgatt ccttcgttgg tactgacaat ggcactggct gccgctctcg
    11641 ggccgagttt atttaacgcc atgctggcga ttgctattgt gcgaattccc ttttatgtgc
    11701 gcctggcgcg ggggcaagca ttagttgtac gccagtatac ttatgttcag gcggcgaaaa
    11761 cctttggcgc gtctcgttgg catctgatca actggcatat tttacgtaac tccctgccgc
    11821 cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc gccacgttgg
    11881 gatttattgg cctgggtgca caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg
    11941 gtcgcaacta tgtactcgat cagtggtggt attgcgcatt tccgggggca gcgattttgc
    12001 ttaccgccgt cgggtttaat ctctttggtg atggtattcg cgatctgctt gacccgaaag
    12061 caggaggaaa gcagtcatga cccaacccgt tctggacatt caacaactgc atttgagttt
    12121 ccccggtttt aacggcgacg ttcacgcgct caacaatgtg tccttgcaga ttaaccgcag
    12181 tgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat
    12241 tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg gggcagattt cactgctggg
    12301 agaagatgtt cttaacgctc gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc
    12361 gatgatcttt caggaaccga tgaccgccct caatccgaca cgtcgaatag gtcttcagat
    12421 gatggacgta atccgccatc atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat
    12481 tgccctgctg gaagagatgc aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt
    12541 tgagctttca ggtggtatgc gccagcgggt aatgattgcg ctggcattct cctgcgagcc
    12601 gcaattgatt attgccgacg aaccgactac ggcgctggac gtcacgctac agttgcaggt
    12661 actgcgtttg cttaaacata aagcccgcgc cagtggaact gcggtactgt tcatcagcca
    12721 tgatatggtc gtggtgtcgc aactgtgcga tagagtttac gtgatgtatg ccggaagcgt
    12781 aattgaaagc ggcgtgacgg cagacattat ccatcatccc cggcatccgt ataccattgg
    12841 tttgctgcaa tgcgcaccag aacatggagt accacgccag ccattacccg ccattccagg
    12901 gacggtacca aacctcaccc atttgcctga aggctgcgct tttcgcgatc gttgctatgc
    12961 ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca acaaccagcg
    13021 ctgcgcctgt tggtatccgc agcaggaggt cataagtgtc taacacgtta ttaacgttat
    13081 gcgacgtcca tatcaatttc ccggcccgta aaaactggct tggtaaaact acggaacatg
    13141 ttcatgccat taatggtatt gatttacaga tccgccgtgg tgaaacctta gggatcgtcg
    13201 gcgagtcagg ctgcggcaaa agcaccctcg cacagctttt aatgggtatg ctgcaaccga
    13261 gccacgggca gtacatccgt tctggctcac aacgcattat gcagatggtg tttcaggacc
    13321 cgctctcttc gctcaatccg cgcttaccgg tgtggcgcat catcacagaa ccgctctgga
    13381 tagctaagcg tagtagtgaa caacagcggc gagcgttagc agaggagttg gctgtgcagg
    13441 tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc
    13501 aacgcatcgc cattgccaga gcactctctt cgcagcctga cgtgattgtg cttgatgagc
    13561 caacctctgc actggatatc tccgtgcagg cgcagatcct caatttactg gtaacgctac
    13621 aggtaaatcg cgggctgacc tatgtgctga tctcacacaa tgtctcggtg attcgtcata
    13681 tgagcgatcg ggtagcggtg atgtatctcg ggcagattgt agaactgggc gacgcgcagc
    13741 aggtgttgac ggcacctgca catccataca cccgattatt gctggattcc ctccccgcca
    13801 ttgataaacc gctggaggaa gaatgggcat tacgtaaaac ggatctgccg ggaaaccgca
    13861 cgttgccgca aggctgtttt ttctacgaac gttgccctct ggcaacccac ggatgtgaag
    13921 tccggcaatc attaacaata agggaggacg gacgtgagat ccggtgctgg cgggcgctgt
    13981 agagatgcta acaacgcatc aggcattcac gcgcctgacg cgtcatccgg caatgcttta
    14041 cgatttcaac tggtaatcaa gcgtaatttc cgctttcagt acctgagaaa ccgggcatcc
    14101 tgcttttgct ttctggatga tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac
    14161 ttcactcttc agtgcgattt tcgtaatcgc aaaaccggca tccactttat ccagcaacac
    14221 atcggcggtg gtatcaatcg atgttggcgt aaatcccgct tcccccagca ttaatgaaag
    14281 cgccattgag aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tagttccttt
    14341 ttcgccttca aaacgcgtgt taaatccata cggctgttgg ttcagcacac cactctcggt
    14401 ggatactgtt cccttcccgc gtttgatatc gccttcccag tgtgcctgac ctttcttatg
    14461 gattgtcatt gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca
    14521 gcacgaggat aagcaggata ttccgaataa aaatccggca gaaatattac ttctgcactt
    14581 aaattaaatt tccacaatag ataaccggga atatataacc caagagatat
//
LOCUS       DAPHDX010000108        14407 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPHDX010000108 DAPHDX010000000
VERSION     DAPHDX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.109"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            305..1876
                     /gene="garD"
                     /locus_tag="RH058_003345"
     CDS             305..1876
                     /gene="garD"
                     /locus_tag="RH058_003345"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV3138893.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            2025..2360
                     /gene="prlF"
                     /locus_tag="RH058_003346"
     CDS             2025..2360
                     /gene="prlF"
                     /locus_tag="RH058_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV3138894.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2360..2824
                     /gene="yhaV"
                     /locus_tag="RH058_003347"
     CDS             2360..2824
                     /gene="yhaV"
                     /locus_tag="RH058_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV3138895.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRFFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2879..3688)
                     /gene="agaR"
                     /locus_tag="RH058_003348"
     CDS             complement(2879..3688)
                     /gene="agaR"
                     /locus_tag="RH058_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV3138896.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3937..5217
                     /gene="kbaZ"
                     /locus_tag="RH058_003349"
     CDS             3937..5217
                     /gene="kbaZ"
                     /locus_tag="RH058_003349"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV3138897.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5240..5713
                     /gene="agaV"
                     /locus_tag="RH058_003350"
     CDS             5240..5713
                     /gene="agaV"
                     /locus_tag="RH058_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV3138898.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5724..6503
                     /locus_tag="RH058_003351"
     CDS             5724..6503
                     /locus_tag="RH058_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV3138899.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6493..7371
                     /locus_tag="RH058_003352"
     CDS             6493..7371
                     /locus_tag="RH058_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV3138900.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7389..7823
                     /locus_tag="RH058_003353"
     CDS             7389..7823
                     /locus_tag="RH058_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3138901.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7820..8953
                     /gene="nagA"
                     /locus_tag="RH058_003354"
     CDS             7820..8953
                     /gene="nagA"
                     /locus_tag="RH058_003354"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV3138902.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9304..10458
                     /locus_tag="RH058_003355"
     CDS             9304..10458
                     /locus_tag="RH058_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV3138903.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10471..11331
                     /gene="kbaY"
                     /locus_tag="RH058_003356"
     CDS             10471..11331
                     /gene="kbaY"
                     /locus_tag="RH058_003356"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV3138904.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11498..11974
                     /gene="agaB"
                     /locus_tag="RH058_003357"
     CDS             11498..11974
                     /gene="agaB"
                     /locus_tag="RH058_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV3138905.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            12013..12816
                     /gene="agaC"
                     /locus_tag="RH058_003358"
     CDS             12013..12816
                     /gene="agaC"
                     /locus_tag="RH058_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV3138906.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12806..13597
                     /gene="agaD"
                     /locus_tag="RH058_003359"
     CDS             12806..13597
                     /gene="agaD"
                     /locus_tag="RH058_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV3138907.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13598..14353
                     /locus_tag="RH058_003360"
     CDS             13598..14353
                     /locus_tag="RH058_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV3138908.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3501 a         3635 c         3747 g         3524 t
ORIGIN      
        1 tgccgtaacg gcaatgaact cgggaattag tataagcagc gcgagaataa taatcattgt
       61 gcaaatgcta atttaattaa tgctatttaa atattatttt gagcatatgc acataaggtt
      121 gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat ccctgaaatg
      181 attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca cccaatgaag
      241 tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg gagaatatag
      301 tacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata taaaagttca
      361 cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag gaacgcgttt
      421 tccggatgga ctggaattaa ttgaacatat tccccagggg cataaagtcg cattgctgga
      481 cattccggct aatggtgaaa ttattcgtta tggtgaagtg attggttacg ccgtgcgtgc
      541 aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag cgccgccgtt
      601 acacacgctg ccactggcaa ccaaagtccc ggccccctta ccgccgctgg aaggatacac
      661 ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac
      721 caccagcgtc cactgtgtgg caggcgtggt ggactatgta gtaaaaatca ttgaacgcga
      781 tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt tgtacggctg
      841 tggcgtggcg attaacgcac cggcggcagt tgtgcctatt cgtaccatcc acaatatttc
      901 gctgaaccct aactttggcg gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca
      961 gcctgagcgc ctgctggtcg gcacggatga tgtgcaagct attccagtag aaagcgccag
     1021 cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg aggatatttt
     1081 gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc
     1141 ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gccttttctg gcgtaacggc
     1201 aaacccggcg gttggctatg cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt
     1261 ctcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg cagtgaacga
     1321 agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc tcaatatggg
     1381 aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt
     1441 ggtagagaag gcactcggct ccattgctaa atcgggtaaa agcgcaattg ttgaagtgct
     1501 gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc cagccagcga
     1561 ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg
     1621 tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcga cccgcaccga
     1681 gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg ccaccggcga
     1741 agaaaccatt gaagaggtgg gctggaagtt gttccacttt attctcgacg tcgccagcgg
     1801 gaagaagaaa accttctcgg atcaatgggg gctgcataac cagctggcag tgtttaaccc
     1861 ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct tagtgccatg
     1921 tttttattgt ttaaagcccc cacgtccatt aataatgcat ttgcattacc ttagttcaag
     1981 cttataattt gagcagaaaa caggctgtaa aaggacagtg aatcatgccc gctaatgctc
     2041 gctctcacgc tgtactgacc actgaatcaa aggtcacgat acgcggacaa acaactatcc
     2101 ccgcgccagt gcgtgaggcc ttaaaactga agccaggcca ggacagcatt cattacgaaa
     2161 ttctgcctgg tgggcaagtc tttatgtgcc gactgggaga tgaacaggag gatcatacta
     2221 tgaatgcatt tttgcgtttt ctggatgcag atatccagaa caacccgcaa aaaactcgtc
     2281 cattcaacat tcaacaagga aagaaacttg tcgctggcat ggacgtcaac attgatgatg
     2341 agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg cgctatatgc
     2401 tcatccctgt tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga cattaaaggg
     2461 aaaagatcct gaaaattatc agagaaaagc cgccacaaag ttattggcgg tagtccataa
     2521 agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc atggcaagtc
     2581 gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg gtcgttatcg
     2641 tttcttcttt cgttatagtg aaaaagagaa agtcatcatt ctgggatgga tgaacgatga
     2701 aaacactctg cgcacctacg gtaaaaaaac agatgcctat accgtattca gcaaaatgtt
     2761 aaaaagagga catcctcctg ccgactggga aagcctcacc caagagacag aagaaaacca
     2821 ttgatggtgt tcacaatgcc cgccgaacgt attcgtgttg accagcgggc accctttctc
     2881 actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga ctatccgcag
     2941 gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc ttatgtaaac
     3001 tggaacgatt gaatttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca
     3061 tccgacggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc tccagatcga
     3121 tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct
     3181 gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc agcagctcaa
     3241 cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt
     3301 cagtgtgctt acgcatcaga cgagcaatct caaaagtggt agtcccggaa tcgaggatca
     3361 cccgatgacc aggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg
     3421 tgttcagcgc gcttttatct tccactgatg gctcgactga cggcgtcgtg ctatcgcaga
     3481 tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac gccagatcat
     3541 tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc
     3601 cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcca gtcactcgct
     3661 tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt
     3721 tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg cccattgacg
     3781 caagctactc tcgtttcagt gactttcatt atgtttcttt tgtgaatcag atcagaaaac
     3841 tattatcttt cgttttattt ttatctcacc atgacgcagt atcaactgaa acaaaacgaa
     3901 agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac agaaatggtg
     3961 agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc acatccgctg
     4021 gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact gattgaagca
     4081 acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc cgattttcgc
     4141 ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt gattctgggt
     4201 ggtgaccatc tggggccaaa ccgctggcaa aacctgccgg ccgctcaggc aatggccaat
     4261 gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca ccttgattgc
     4321 agcatgtcct gtcaggacga tccgattccc ttaactgatg acatcgtggc tgaacgcgcc
     4381 gcccgtctgg cgaaagtggc ggaagaaacc tgtcttgaac actttggcga agccgatctg
     4441 gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac cttaagcgag
     4501 ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg tcacgccttt
     4561 gaaaagcaag gtttgaatgc catctggcca cgcatcattg ccctggtggt tcaacccggc
     4621 gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgag cgccttaagc
     4681 cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga ttatcaaacg
     4741 ccgcaatcgc tgcgccagct ggtgattgac cactttgcca ttctgaaagt tggcccagcg
     4801 ctgaccttcg ccctgcgtga agctctgttc tctctggcgg cgattgaaga agaactggtg
     4861 ccagcgaaag cctgttctgg tctgcgtcag gtgctggaag acgtgatgct cgaccgcccg
     4921 gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttat
     4981 agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga cgctttcgct
     5041 catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag ccagtatctg
     5101 ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg ggaactcatt
     5161 atcaaccata ttcaggacat cctggcgcag tactacacag cctgtgaagg ccaataagca
     5221 aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg aacgcttgat
     5281 tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg tgctggtagc
     5341 caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg tactggcaga
     5401 agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc atcgcgccgc
     5461 cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga cgctggtgaa
     5521 aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcaa atggcaaaca
     5581 acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta acgacctgaa
     5641 agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg tggacctctt
     5701 taaattactt tgagggattc atcatggaaa tcagcctgtt gcaggcattt gcgttgggca
     5761 ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacacatatg caccgcccgg
     5821 tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga attttaaccg
     5881 gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca cagccgccta
     5941 acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc gtgaaacccg
     6001 atgtcgcggt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg attaccttcc
     6061 tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag aacgccgata
     6121 ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact ttctattttc
     6181 tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc attgatgtcc
     6241 tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt
     6301 ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc atcctgggct
     6361 ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct gcgctggcga
     6421 tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag
     6481 aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga aaacgaagaa
     6541 acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg cgcggagctg
     6601 acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac
     6661 tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct gaaaaagatc
     6721 cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc
     6781 catccgtttc tggtgacatt tgttatcggc attatccttg cgatggagcg ttctaagcag
     6841 gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgctcgg cgggattggc
     6901 gatgcaatgt tctggctaac gctactgccg atttgtggcg ggattggagc cagtctggca
     6961 ttgcaaggct ccattcttgg cgcagtcgtc tttattctgc tgttcaacgt ggtacacctg
     7021 gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc gattccactc
     7081 attaaagcga ataccaaaaa agtcggccat gctgcgtcta tcgttgggat gacggtaatc
     7141 ggcgcgctgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac agcgggcgac
     7201 gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt cttaccgctg
     7261 gtctacacct tgaccatgtt ctggctggta cgccgcggct ggagtccgct gcgtctgatt
     7321 gcagttaccg tggttctcgg catcgtcggt aaattctgcc atttccttta aatacaatga
     7381 ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagc ggcatggaaa
     7441 aagcaatgaa gcaaattctc ggtgagcaat cgcagtttat cgccatcgat tttccggaaa
     7501 cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa ctggattgtg
     7561 aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc gtagcttcaa
     7621 cgctggcgat gcaaaaaccg ggctgtgaag tgatcaccgg caccaatttg caactgctac
     7681 tggagatggt gctggagcgc gaagggttaa gcggtgaaga attccgtgta caggcgctgg
     7741 agtgtggaca ccgagggctg accagtctgg tggacgagtt aggccgctgc catgaagaat
     7801 gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga
     7861 gggatggctc gatgaccatc agttgcgtat tgctgacggt gtcatcgcag caatcgaacc
     7921 gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccccgctt acatcgacac
     7981 ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg tactcgacaa
     8041 gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttgccgacta ccgtaaccgc
     8101 gccgcttaac agcattcatg cggcgctgaa acgtattgct caacgttgcc aacgcggcgg
     8161 acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc cgcagaataa
     8221 aggcgcgcat ccgccggaat tgtttcgcga gcttgaaatt gccgagctgg atcaattgat
     8281 tgccgtttct cagcacacct tacgcgtggt agcgctggca ccggaaaaag agggggcatt
     8341 gcaggccatc cgccatctta aacagcaaaa tgtacgagtg atgctggggc atagcgcggc
     8401 gacctggcaa caaactcgcg ccgcgtttga tgctggtgcc gacggcctgg tgcattgcta
     8461 taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg gattaacgga
     8521 caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg cggcgatgtc
     8581 gctgtgttgt tgctgtgcaa aagagagaat cgtgctgatc accgacgcga tgcaggcagc
     8641 cgggatgccg gatggtcgct atacgttatg tggcgaagaa gtgcagatgc acggtggcgt
     8701 tgtccgtacc gcgtccggtg ggctggcggg cagtacgctg tctgttgatg cggcagtgcg
     8761 caacatggtc gagttgacgg gcgtaacgcc tgcggaagcc attcatatgg catcgctgca
     8821 tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg gcaaacgcgc
     8881 cagcatcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc agagtcaatt
     8941 agcttcgttt tgatattttg ctcctttatt gggccttcac ttcccccgta aggcctttct
     9001 ttttctttcg ttttgatctg tgcagaggtg tcggatgcga cgctaacgcg tcttatgcga
     9061 cctacggtac gccgcatcca gcaatcggtg aacatagcga cgtcattacg caattcttta
     9121 atcttccctt gtcttttctt tcaatgatca cagatttcgt tttatttcct ttttctccat
     9181 tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc aagtgaaatg
     9241 aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac taaggattga
     9301 gttatgccag aaaattacac ccctgctgcc gccgcaaccg gtacatggac tgaagaagag
     9361 atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc gctacgttct
     9421 gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat catcctgacc
     9481 ggagccggaa cctcggcatt tatcggtgac atcatcgcgc cgtggctcgc cagccatacc
     9541 ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat ggactacctg
     9601 aacccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag cccggaaagc
     9661 gtcgctgccg tggaactggc aaatcaattt gtaccagaat gctatcacct gccgatcacc
     9721 tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc gtttgccctg
     9781 ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat taccaccatg
     9841 atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac cttccgcgac
     9901 gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga aggtgtgttt
     9961 ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca gggcgcagca
    10021 cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc cttctatgat
    10081 tccccgaccg gattccgtca tggcccgaaa tcactggtcg ataacgaaac gctggtggtg
    10141 gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct ggctgaactc
    10201 cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac cgacatcgtc
    10261 gctgccggtc cacatattat cctgccgccg tcacgtcact ttatcgacgt tgagcaggca
    10321 ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca catgggcaat
    10381 acgccggata ccccatcagc cagcggcacc gttaaccgcg tggtgcaagg cgtaatcatt
    10441 catccgtggc aggcataaga ggatcgcatt atgagcatta tctccactaa atatctgtta
    10501 caggacgccc aggccaatgg ctacgcggtg cctgctttta acattcataa cgccgagacg
    10561 atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct cgccggaacg
    10621 ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgcag cgcctattcc
    10681 acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct ggatgatatt
    10741 cgccgtaaag tccacgcagg tgtgcgcagc gcaatgatcg acggcagcca cttcccgttt
    10801 gccgagaacg tgaagctggt gaaatcggtt gttgacttct gccactcaca ggattgcagc
    10861 gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt tgacgccgaa
    10921 agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac tggcgtcgac
    10981 agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc gaagattgat
    11041 ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct acatggtgcc
    11101 agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac aaaagtgaac
    11161 gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt tgcggaaaat
    11221 ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat gaaagaagtt
    11281 gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata atcattagtt
    11341 ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtgcctt aatggaaaag
    11401 gagatgacta aaccttaata aatacatcag tacaatatcg caacaataat atattaaaaa
    11461 aaattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa atattctctt
    11521 aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga catccaccat
    11581 cggtgcgaat ctgctggtag tcgtggatga tgttgtcgct aacgatgata ttcaacagaa
    11641 attaatgggt attaccgcag aaacctacgg ctttggcatt cgtttcttta ctatcgaaaa
    11701 aaccattaac gtcatcggca aagctgcacc gcatcagaag atcttcctga tttgccgtac
    11761 gccacaaacg gtacgtaaat tggtagaagg tggcattgac ctgaaagata tcaacgtcgg
    11821 caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg tcgatgacca
    11881 ggatctcgcg gacttacgtt ttattaaaca acgtggcgtg aatgttttca ttcaggacgt
    11941 ccctggcgat caaaaagaac aaatccctga ctaaatctaa aatcgcctta atattggttt
    12001 gaggtaataa aaatgcatga aataacccta cttcagggat tatccctggc ggcgttagtt
    12061 ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc gataatcgtt
    12121 tgtaccctaa ctggcgctat tctcggtgat attcagactg gcttaattac cggtggtctg
    12181 acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc caacccgatt
    12241 atggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc caaaacagca
    12301 attggtcttg gcctgccgtt tagtttgtta atgcagtacg tcattctgtt cttctattcc
    12361 gctttctcat tatttatgac caaagccgat aaatgcgcga aagaggcgga tacggcagcg
    12421 ttttcccggc ttaactggac aacgatgctc atcgtcgctt cagcgtatgc ggtgattgct
    12481 ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc gatgcccgcc
    12541 tggttgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg ttttggcttg
    12601 ctgctgcgcg taatgttcaa agcgcaatat atcccttacc tgatcgccgg tttcctgttt
    12661 gtttgctaca tccaggtcag caacctgttg ccggttgccg tactgggcgc aggctttgcg
    12721 gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc cgttgccagt
    12781 aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag atatcacccg
    12841 tctgggcttt cgttcgtcgc tgctgcaagc gagctttaac tacgaaagga tgcaggcggg
    12901 cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataaggacg acaaaccggg
    12961 cttaagcgcg gcgatgaaag ataacctcga atttattaat acccacccga atctggtcgg
    13021 attcctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg acaccattaa
    13081 aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga ttttctggtt
    13141 tactttgttg ccgattatgg cgggaatttg ctcatcattt gccagccagg gaaacctgct
    13201 ggggccgatt ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg tcggctggac
    13261 ccacgtcggt tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga actcgcagat
    13321 gattgctcgc tcggcaacca tcctcgggat cacggtaatc ggcgggctga tcgcttcgta
    13381 tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg tcgcgctgca
    13441 acaggatttc ttcgataaag tcttcccgaa cattttaccg atggcctaca ccctgctgat
    13501 gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg tgacttttgt
    13561 gctctctatt gtttgttccg cattcggcat tttgtaaatg gaacgaggca ctgcttctgg
    13621 cggtgcctct ttacttaagg aatttcatcc tgtgcaaacc cttcagcaag ttgaaaacta
    13681 tacggcgtta agtgaacgag ccagcgaata tttattggct gtgatccgta gcaaaccgga
    13741 tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt atctggtaga
    13801 aaaaatccac cagcagcagg ttgatatcag ccagctcacc ttcgtgaagc tcgacgaatg
    13861 ggtggatctg ccattaacga tgcctggcac ctgcgaaact ttcctacaac agcatatcgt
    13921 gcagccgctg gggctgcgtg aagaccagct catcagcttt cgctccgaag agataaatga
    13981 gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg atttatgcgt
    14041 tctaggattg gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa gcctacaacc
    14101 ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt taaaaaccac
    14161 gggtcgcccc gtgactcgtg ggatcacctt aggcctgaag gatattctca atgcccgcga
    14221 ggttttgtta ttggtgactg gcgaaggaaa gcaggatgcg acagaacgtt ttctcacggc
    14281 taaagtctcc accgctatcc cggcttcgtt tttatggctg cacagtaact ttatttgttt
    14341 aattaatacc taaccagatg aaaatttgct caaaattttt aaattaactt attcaacagt
    14401 cacgcat
//
LOCUS       DAPHDX010000109        14396 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPHDX010000109 DAPHDX010000000
VERSION     DAPHDX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.110"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(163..699)
                     /gene="rcdA"
                     /locus_tag="RH058_003361"
     CDS             complement(163..699)
                     /gene="rcdA"
                     /locus_tag="RH058_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV3138909.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            783..1991
                     /locus_tag="RH058_003362"
     CDS             783..1991
                     /locus_tag="RH058_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3138910.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1991..2806
                     /gene="ybjI"
                     /locus_tag="RH058_003363"
     CDS             1991..2806
                     /gene="ybjI"
                     /locus_tag="RH058_003363"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV3138911.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            2892..3176
                     /locus_tag="RH058_003364"
     CDS             2892..3176
                     /locus_tag="RH058_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138912.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3217..4449)
                     /gene="mdfA"
                     /locus_tag="RH058_003365"
     CDS             complement(3217..4449)
                     /gene="mdfA"
                     /locus_tag="RH058_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV3138913.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            4734..5330
                     /gene="ybjG"
                     /locus_tag="RH058_003366"
     CDS             4734..5330
                     /gene="ybjG"
                     /locus_tag="RH058_003366"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV3138914.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5388..6146
                     /gene="deoR"
                     /locus_tag="RH058_003367"
     CDS             5388..6146
                     /gene="deoR"
                     /locus_tag="RH058_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV3138915.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(6193..7395)
                     /gene="dacC"
                     /locus_tag="RH058_003368"
     CDS             complement(6193..7395)
                     /gene="dacC"
                     /locus_tag="RH058_003368"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV3138916.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7642..8268
                     /gene="gstB"
                     /locus_tag="RH058_003369"
     CDS             7642..8268
                     /gene="gstB"
                     /locus_tag="RH058_003369"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV3138917.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(8265..9380)
                     /gene="yliI"
                     /locus_tag="RH058_003370"
     CDS             complement(8265..9380)
                     /gene="yliI"
                     /locus_tag="RH058_003370"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV3138918.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVADFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(9491..9874)
                     /gene="bssR"
                     /locus_tag="RH058_003371"
     CDS             complement(9491..9874)
                     /gene="bssR"
                     /locus_tag="RH058_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV3138919.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            10087..11412
                     /gene="rimO"
                     /locus_tag="RH058_003372"
     CDS             10087..11412
                     /gene="rimO"
                     /locus_tag="RH058_003372"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV3138920.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(11459..12787)
                     /locus_tag="RH058_003373"
     CDS             complement(11459..12787)
                     /locus_tag="RH058_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV3138921.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(12795..>14396)
                     /locus_tag="RH058_003374"
     CDS             complement(12795..>14396)
                     /locus_tag="RH058_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3138922.1"
                     /translation="SVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAF
                     IIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKN
                     SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEA
                     VFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFD
                     SSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIE
                     ENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITI
                     AEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTI
                     MKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYE
                     INPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCV
                     QAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         3646 a         3646 c         3407 g         3697 t
ORIGIN      
        1 caaccgggtt gttccaaatg gcgacaaagt ggcggcagcg gttggcatta ccccgtaata
       61 accaccactg accaccaacc caacttattg tttttactct aacttattgt tttcattaac
      121 ccgtttacat aaatgggttt tttgttatct aaaacttatc tactaccctg caaccctctc
      181 aaccatcctc aaaatctcct cgcgcgatag cggcttacgg tcggtgacaa aatgcagcgt
      241 catcccctca ataaacgcat caagcgcgcg ggcggttccg ggttcaaacc attgttcgag
      301 cgtttgctga ctgcgctgca tccagttttg cattaccgtt tttaatagcg gttttcggct
      361 agccagcgcg tagagctggt acatcagctc catattatcc ggcgttgcaa cctgtgagct
      421 gtagatcata tcggtgatag cctggcatgc gccttgagca tcactaacat cgctaaaaaa
      481 tgcctgatat tgccgggaca taatctcagt aaaactgctg aacgcctcca gcaacaactc
      541 atcaattcct gaaaaatagt aggtcatcga tcccaacggt accccggcaa gggtagcaat
      601 tttgcggtgc gtaacagcat gtattccgta aagtttcacc gcctccagcg tggcctggat
      661 aattttttct cgccgttgcg gatcgttagc gcgacgcatg tcatttcctc tctctgcaat
      721 tgtgtacaaa tgtacacaac cttgctaatg ttgtcttccc tcttcttatt ttgacgtgat
      781 ctatgaccgt aaattcttca cgtaatgcat tgaaacgccg aacctgggcg ctgtttatgt
      841 tcttcttttt gccaggcctg ttaatggcgt cttgggcaac ccgtacgcct gctatccgcg
      901 atattctctc tgtctcgatc gctgaaatgg gcggtgttct ctttggtctg tcgatcggtt
      961 cgatgagcgg tattctctgc tcggcgtggt tagtgaaacg ctttggcacg cgtaatgtca
     1021 tcctggtcac gatgtcctgc gcattgatcg ggatgatgat attaagtctg gcactctggc
     1081 tgacatcgcc cctgctcttt gccgttggtc tcggcgtctt tggggcaagt tttggttctg
     1141 cggaagtggc gataaacgtt gaaggtgccg ccgttgagcg agaaatgaat aaaacggttt
     1201 tgccaatgat gcacggtttt tatagcctgg gcacgctggc aggcgctggt gtcgggatgg
     1261 cactgacggc ctttggcgtt ccggcaacgg tgcacatttt attggcggcg ctggtaggta
     1321 tcgcgcctat ttatatcgcc attcaggcaa tccctgacgg tacgggcaaa aatgctgccg
     1381 atggcaccca gcatggcgaa aaaggcattc ctttttatcg cgatatccag ttgctgctga
     1441 taggtgttgt ggtgctggcg atggcctttg ccgaaggttc tgccaacgac tggttaccct
     1501 tattaatggt tgacggtcac ggttttagcc ctacttccgg ctcgctgatt tatgccggtt
     1561 ttaccctggg gatgaccgtt ggacgcttca ccggcggttg gttcatcgac cgttacagtc
     1621 gcgttgccgt ggttcgggcc agtgcactaa tgggggcgtt gggtattggg ctgattattt
     1681 ttgtcgatag cgcctgggtc gctggggtgt ctgttgtact ctggggactg ggtgcctcgc
     1741 tgggcttccc gctgaccatt tctgccgcca gcgataccgg ccctgatgca ccaacccgcg
     1801 tcagtgtggt agcaacgacc ggttatctgg ctttcctcgt cgggccgccg ctgctgggct
     1861 atctcggcga acattatgga ttacgtagtg caatgctggt tgtactggcg ctggttattc
     1921 tcgcggctat tgtcgcgaaa gccgtcgcca aacccgatac caaaacgcag acggcgatgg
     1981 agaatagttg atgagcatta aattaattgc ggtagacatg gatggtactt tcttaagcga
     2041 tcaaaaaacc tataaccgtg agcggtttat ggctcagtat cagcaaatga aagcacaagg
     2101 aattcgcttt gtagtcgcca gcgggaatca atattatcag ttgatctctt tcttccctga
     2161 aattgctaat gaaattgcct ttgtggctga aaacggcggc tgggtagtga gcgaaggcaa
     2221 agatgttttt aatggcgagc tgtcgaagga tgcgtttact actgtcgtgg aacatttgct
     2281 gacgcgcccg gaagtggaaa ttattgcctg cggaaaaaat agcgcctata cgctcaaaaa
     2341 atatgacgat gccatgaaaa cggtggcgga aatgtattat caccgtctgg aatacgtcga
     2401 taactttgac aacttagagg atatcttctt taagtttggc ctgaatcttt ccgatgaact
     2461 gattccacaa gtacaaaaag cattacatga ggccatcggc gatattatgg tgccggtcca
     2521 caccggcaac ggcagcatcg atctgattat ccccggcgta cataaagcca atggccttcg
     2581 ccaactgcag aaattatggg gaatagacga cagcgaagtg gtggtctttg gcgatggcgg
     2641 taacgatatt gagatgctgc gtcaggcagg ctttagtttt gcaatggaaa atgccggcaa
     2701 tgcggtcgtc gcagcggcaa aataccgggc aggctccaat aaccgtgaag gcgtactgga
     2761 tgtgatcgat aaagtgctta aacacgaagc gccatttgac caataaaaac ggcagcacgc
     2821 ccggcaacac aatacctgaa tgaaggctgc ataattcgcg gaaattaacc cgctgcatta
     2881 tttcaggcga aatgtttatg aaaaattgtc tactgttggg cgcgctttta atgggcttta
     2941 ctggtgtggc gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac aaagtggttg
     3001 tggttcctga ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg ccgcaaaccg
     3061 tctatgtggc tcctgcgcca gctccggctt accgcccgca tccttatgtt cgccatcttg
     3121 ccagcgtagg tgaagggatg gttatcgaac accagattga cgaccatcac cattaattcg
     3181 agaatgcctg atcgcacaaa gcaatcaggc atttttttac ccttcgtgag aatttcccat
     3241 ctgtttatct tttaaaaaga taaccatcag cgacagccac aaaattccgt tgacaaggtt
     3301 gaagagatta aacagtccgt tgcccccgtt cagccaggca tgtttgctga tttcaatacc
     3361 gacggtaaag atcagcattt gcagcattcc catcgccgca gaaaccgtac ctttactcat
     3421 atcgctggca aacagggtta atcgcaccag tcccgcattc gccagaccaa tgccgaaagc
     3481 ataaatactt aacccggcgg tcatccataa atacgcgtgc gatgagataa ccgttgccgc
     3541 agcagcgacc aacagaccaa taataatcgg ccagccgccc ataataatca gcgaacgtac
     3601 ggtgcggcgc gaggtcagac gcgctaacag caagttaccc gcaattaacg ccccgaaaat
     3661 aggcacttgc agcaagccat attcatagct gctcaactgc tcgccggtaa tgatgataat
     3721 cggcgactgg gcgatccacg ccagcaatgg caggctaacg aatcccagcg ccagcgcccc
     3781 cgccacaaag cggccgttct tcagcaccag cttatagtca cgaccgagtt ctttcagtga
     3841 cagtttctcg cctatacgcg tggcggtttc aggcatggct tgttgcagac cgaaaaagga
     3901 gatcgctgcc aatgcggcaa acaagacaaa catcccttcc cagggcagca catggatcca
     3961 cgccgcgccc accagcggac caagtagcgg agcaatcagc gccacgttcg ccatcagcgc
     4021 ggtgatcttg atacaaaccg cctcttcgaa ggattcctga attgcggcgt atcccacagc
     4081 gccaatgaaa cagaggctta tgccctgcaa gaagcgcaac agagtgaatt gttcaatgtt
     4141 ttgcgccagt aatattgcca gacaggtgat gataaaccac accacccccg ccagcatcac
     4201 cggacggcga ccaatacgat ccgacagcgg ccccaggagc cattgtaaaa acatcccgcc
     4261 cgccagatat gcggtcattg aagtaggaac ccaatcaatg cccgcctgat attgttccac
     4321 cacggccaac ataccgggtt gaatcatatc gttgccgata taggttgaaa attcgtaaag
     4381 caccagacag agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg aagctaattt
     4441 attttgcatg caatttcttc gccaataata atcgcgcaga gtttaataaa agcacagcta
     4501 acgagaaagc gaattttgta gctgaaacca cggttaagca cattcttaca ttattgcgag
     4561 tatagctacg ctttctttaa gttttattta acctatgccc gttacaatta cccaccgtaa
     4621 acaggccgct tgagggaaat aagacgatgc cgctttaccc agtttaacct gcactttatt
     4681 ctcaacgact tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa gttatgctgg
     4741 aaaatttgaa tctctctcta ttctctctta ttaacgcgac gccagactcg gctccgtgga
     4801 tgatctcgtt ggcgattttt attgctaaag atttgattac cgtggtaccg ctgctggccg
     4861 cggtgctttg gttgtggggg cttacagcac aacggcaact ggtgataaaa atcgctatcg
     4921 cgctggcggt cagcctgttt gtgtcctgga cgatgggaca tctttttccg cacgaccgac
     4981 cctttgtcga aaatatcggc tataacttcc tgcatcatgc ggcggatgac tcattcccaa
     5041 gcgaccacgg tacggtgatt ttcacctttg cactggcatt tttatgctgg catcgcctgt
     5101 ggtccggctc acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc gtttatcttg
     5161 gcgtgcactg gccgctggat atgctcggtg gattgctggc aggtatgatt ggctgcctta
     5221 gcgctcagat tatctggcaa gcgatggggc ataaactcta tcaacgtctg caatcgtggt
     5281 atcgcttctg ttttgcatta ccgatccgca aaggctgggt gcgtgactga tctctgctaa
     5341 aaagtgtagt attgagcggc tcgcttcaat aactattcag agggattatg gaaacacgtc
     5401 gcgaagagcg tatcgggcag ctgctgcaag aattaaaacg cagcgataag ttacatctta
     5461 aagacgccgc cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat ctgaacaacc
     5521 acagtgcgcc cgtcgttttg ctcggcggct atattgttct ggaaccgcgc agtgccagcc
     5581 attatctgtt aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg gcggcaaaac
     5641 tggctgcgac gctggtagaa cccgatcaga ccctcttttt tgactgtggc accaccacgc
     5701 cgtggattat tgaagcgatt gataatgaaa tacctttcac cgccgtttgt tattcgctaa
     5761 atacctttct ggcgctgaaa gagaaacccc attgccgcgc atttctgtgc ggtggtgaat
     5821 ttcacgccag caacgccatt ttcaaaccca tcgattttca gcaaacgctg aataattttt
     5881 gcccggatat cgctttttat tctgcggcgg gcgtgcatgt cagtaaaggc gctacctgtt
     5941 ttaatcttga agagttgccg gtaaaacact gggccatgtc gatggcgcaa aagcatgtgc
     6001 tggttgtcga ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt gacctgaaac
     6061 gctttgatat tgtggtgagc gattgttgcc cggaagatga gtatgtgaag tacgcgcaga
     6121 cgcagcgcat taagttgatg tattaatgac gtataaccgg atgacgtttc gcgccatccg
     6181 gttatcagaa gattaagaga accagctgcc gaaccactga tggaatttca tcatcacgaa
     6241 atcccacatc cgaccaaaga atccgccctc ttccacattt tccatcacga tcagcggacg
     6301 ctgctcaatg gatttaccgt taagctggaa atcaatggtc ccgacaacct gacctttttt
     6361 cagcggtgcg gtaagctgcg gttccgttaa cgtataactc gctttcaggt ttttcagctg
     6421 cccacgcggt atggtcactg agcccgcttc acctgccccg agattcactt cgctcttatc
     6481 accaaaccag acgcgctgag tcacaaaggt ggcatcaggt ttaattggcg tcacggtttc
     6541 aaagaagcgg aaaccccagg tcaataattt ctcagactca ttaaaacgga tacggtcggt
     6601 tttcgccccc aacactacgg agattaaacg catatcgccc tgggtagccg aagcaaccag
     6661 attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct
     6721 ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt
     6781 atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc
     6841 gcgcgcggtg ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt
     6901 ggtcagaccc agttttttgg cataaccatt catcagacca ataaatgact cctggctccc
     6961 ggcaacgtaa tcagccagcg caatacaggc gtcattaccg gactggataa tcacaccttt
     7021 gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg
     7081 cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt
     7141 aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct
     7201 cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc
     7261 cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt cggccgcgaa
     7321 tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga
     7381 ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc
     7441 gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta
     7501 aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc
     7561 cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag tctgtggctg
     7621 cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc aaccaacgta
     7681 aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc
     7741 cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg
     7801 ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc
     7861 tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg
     7921 gaagcggaaa aatggatgga ctgggcaaac cagacactca gtaatgctca tcgcgggatc
     7981 ctgatgggat tagtcagaac accacccgaa gagcgcgatc aggccgccat tgatgccagt
     8041 tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg
     8101 ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg
     8161 tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc
     8221 actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta
     8281 actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca
     8341 gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact
     8401 ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc tataaataat
     8461 ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc
     8521 gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc
     8581 ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc gtagtttttg
     8641 cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt cagccacagt
     8701 gcattactcc acggattcat cgccattcct tgcggattac gaatgccata agaccagatc
     8761 tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc gccctggtcg
     8821 gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg
     8881 ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg cccgccaaaa
     8941 tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc ggccactttt
     9001 gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc atcatcgcca
     9061 acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc taaaaccacg
     9121 tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat
     9181 aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg
     9241 tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg cagtacttcg
     9301 acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg
     9361 aaaaaggatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc gtagtcagtg
     9421 gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga cgctttctcc
     9481 cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg atactcagcg
     9541 ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca gtcgggcaat
     9601 catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa
     9661 gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta tcgtgcagtt
     9721 cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg gacatgtatc
     9781 cttttgcctt cctcagttgc acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt
     9841 tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct ttccagcctt
     9901 gttgcaatta aatagtatgg ctattttttg agcaaaatgc tgtccaggat caattcttca
     9961 cgccaaatta acaaaattta catttccttc gcctccgcca gcgaatgaga ttgcgataca
    10021 aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact gaatattttt
    10081 taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct tggctgtccg
    10141 aaaaaccttg tcgattcaga gcgtattctc accgaactac gcactgaagg ttatgacgtg
    10201 gtaccgagct atgacgatgc ggacatggtg atcgtcaaca cctgcggctt tattgacagc
    10261 gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg caaggtaatt
    10321 gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc gaaagtgctg
    10381 gaaatcaccg ggcctcatag ctatgagcag gttctggagc acgttcatca ctacgtgcca
    10441 aaaccgaaac acaacccatt cctgagcctg gtgccagaac aaggtgtgaa actgacaccg
    10501 cgtcattatg cctatctgaa aatttcagaa ggctgtaatc accgctgcac cttctgcatt
    10561 attccgtcta tgcgcggcga cctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg
    10621 aaacgtctgg tagatgcggg cgttaaagag attctggtga tctcgcagga tacttccgcc
    10681 tatggcgttg atgttaaaca tcgtactggc ttccacaacg gcgagccggt aaaaaccagc
    10741 atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct gcactacgtt
    10801 tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg
    10861 tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg
    10921 ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg
    10981 accctacgct caacctttat tgtcggcttc cctggcgaga cagaagaaga tttccagatg
    11041 ctactagact tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa atacagcccg
    11101 gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt taaagaagaa
    11161 cgctggaacc gtttcatgca gctgcagcag cagatttccg ccgagcgcct gcaagagaaa
    11221 gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc gattggtcgc
    11281 agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga aaccaacgtt
    11341 aagccgggtg atatcctgcg tgtgaaagtc gagcacgccg atgagtacga tttgtggggt
    11401 agccgggttt aattaattca ggccgggcaa aatcatttgt ccggcctgcg aataatgatt
    11461 actgccagct aatctgagcc ctgccgtttt tcttactctt atacagccgc tcatccgcca
    11521 gcattttcaa gcgttcgacg gtggtacatt catttgaatg cgctacaccg atactggtac
    11581 ttaaattaat gcttcccacc tggaaatgaa cgatttcttt tagaatcgat agcaactctt
    11641 ccagcgttcc acccgtataa atcaccgcaa attcgtcacc gccgaagcgg tagattttac
    11701 ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac acttcatcgc
    11761 ccagcaaatg accgaaggtg tcgttaatgt ttttgaattt atcgacgtca aacaggcaca
    11821 aataaccgcc tttttccggc agcgctttta aatcttcgtc aaaggatttt ctgcccattg
    11881 ctttggtcag gtcgtccagg aacgccgagt tatacatact gtagagtttt ttgacatcgg
    11941 cagccgaaaa gaaaagcccg ccatccagta cgcgttcaaa atggttatta atgatggctt
    12001 ctttctccac aataccacga ttaatcactc tgcgtaccag atagaggcaa ctggtacccg
    12061 atatcactgc caccaatgcg gcaaagaaaa taatcaatgt acagacccgc aaataaacat
    12121 cttttttata cgcaaatacg tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt
    12181 ccgaaacggt gaagatggcg tttttagcat tatattcatc accggtcacc ggtttaccgt
    12241 tcttaaatag agagaacatc aggttctcgc tgtccatcgc cagcgatttt gacagtaaag
    12301 aaaaacgctc cagaccaagc aaaaatatca gactacgatt gtgagttacc ggatagaggt
    12361 aatagacgaa ggttttaccg tcaaaagaga taaaccctgt atttttgaca tgtcgatact
    12421 tcacaacctg cataatatct gcacaataat ttttctcgaa ctcctttacc tgaatacatt
    12481 cgtcactgga aatcagataa ggaccgacat cgtacaaata agagggggaa accgagtcgg
    12541 cgtaagctgt gcgcaaacca accaacagct gttcctccgt gggatttagc gtgttgataa
    12601 gatcaattgc cacgctacgc cgatattcag caatatttgc caccgcaaaa gcagtatcaa
    12661 taacggactg tttcaggcta tacacccgtt cctttaccat ttgagtgtag atcccgcagg
    12721 caactgctga aatcatgata aaaatcagca gactgactgt aagtacgaac ttattgattc
    12781 tggacatacg taaactactc ttttactaat tttccacttt tatcccaggc ggagaatggc
    12841 atgggtttct ggtacagata cccctgagcc tgaacacatc ctaatttaat taattcgcgc
    12901 gcaacgtctt cgctctctac gccttccgca atcaccggga tccggttata gcgtgccaga
    12961 tcgaaaataa tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag
    13021 tccaccttta tgctgtctgg attaatttca taaaaacgtt tcaggtttga caaaccggtg
    13081 ccaaagtcat caatcgatat ccgtacccct tgtttacgca gttgttcaat attgagataa
    13141 aaatgttcat taattcgctc gatcacggtt tcagtgatct caacacagag gctgttcgcc
    13201 ttcaggccat tttctttcat gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac
    13261 tgccagggcg aaacattaat atgcagtaag aaatcatcct tcagcgacgc acgctgacta
    13321 aaatgtctga attcacccat tgccgtttta ataatctgat aacccagctc attgataaac
    13381 ccgatatctt cagcaatggt aatgaacttc agcggtgaaa tgatcccccg ctccgcagaa
    13441 acccaacggc acaacgcctc cgcgcccaga atggcgcggg tgttaatatc aacgatcggt
    13501 tgatagacga ggaatagttc gccaatttcc attgcatggc gaatattacg cgcaatccag
    13561 atattctctt ctatttcgtt gagtttcgcg tcatcacaaa aaataaactc ggagaacata
    13621 tggtcacgga tactggaaat cgccagtttc agtttttctg cgtaactcca ggcgttttcc
    13681 ccctgcaaag gttcacaaat ggcagactta tggatgcgca gattgcggta gccaaagctg
    13741 ctgtcaaaat cgtttaccat cgctatttta cgggtaaatg tctgtacatt ttcccgacac
    13801 agaagtaaat agagatcggc attgaatgtc acgatatcgc agcattgcgc atatttctcg
    13861 gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc aaacaccgct
    13921 tccacggctt ccagaccaca cagtttgatg atccccacag ccagatactg attattgttg
    13981 gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga gatcagtgag
    14041 agcaatttat ttgaccgttc aatttcggca tctttcattt ccgcgatcga atttttgatg
    14101 ctgataatct ctttcgcatc gccttcgcga attttcagag cctcgatttg atccgttgag
    14161 tcgggcaaaa acgccagcgt attaaccagc tcggtcattt cagaaaattg ccgacggaac
    14221 acacgagtgg tcatgaaata taccaccagc aacacgataa taatgaaagc aaacggtaga
    14281 taaaaaagtt tatctgatgc gttcaataaa ttaccgccag gataataaaa atagatttcg
    14341 aacaatgcat tatttggcac tgctgatgtc tttgcaatat actccgtatc gacaga
//
LOCUS       DAPHDX010000110        14386 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPHDX010000110 DAPHDX010000000
VERSION     DAPHDX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.111"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..612
                     /locus_tag="RH058_003375"
     CDS             46..612
                     /locus_tag="RH058_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3138923.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            663..3122
                     /locus_tag="RH058_003376"
     CDS             663..3122
                     /locus_tag="RH058_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3138924.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWHGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3137..3868
                     /locus_tag="RH058_003377"
     CDS             3137..3868
                     /locus_tag="RH058_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3138925.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3865..4926
                     /locus_tag="RH058_003378"
     CDS             3865..4926
                     /locus_tag="RH058_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3138926.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     PPIEAGPFDTVVLFKINYN"
     gene            5078..6124
                     /locus_tag="RH058_003379"
     CDS             5078..6124
                     /locus_tag="RH058_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV3138927.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6121..6912)
                     /gene="nei"
                     /locus_tag="RH058_003380"
     CDS             complement(6121..6912)
                     /gene="nei"
                     /locus_tag="RH058_003380"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV3138928.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQAADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6948..7682)
                     /gene="pxpA"
                     /locus_tag="RH058_003381"
     CDS             complement(6948..7682)
                     /gene="pxpA"
                     /locus_tag="RH058_003381"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV3138929.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7672..8604)
                     /gene="pxpC"
                     /locus_tag="RH058_003382"
     CDS             complement(7672..8604)
                     /gene="pxpC"
                     /locus_tag="RH058_003382"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV3138930.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8598..9254)
                     /gene="pxpB"
                     /locus_tag="RH058_003383"
     CDS             complement(8598..9254)
                     /gene="pxpB"
                     /locus_tag="RH058_003383"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV3138931.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9277..10020)
                     /gene="ybgI"
                     /locus_tag="RH058_003384"
     CDS             complement(9277..10020)
                     /gene="ybgI"
                     /locus_tag="RH058_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV3138932.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLVADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10291..11772
                     /gene="dtpD"
                     /locus_tag="RH058_003385"
     CDS             10291..11772
                     /gene="dtpD"
                     /locus_tag="RH058_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV3138933.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11814..13232)
                     /gene="phrB"
                     /locus_tag="RH058_003386"
     CDS             complement(11814..13232)
                     /gene="phrB"
                     /locus_tag="RH058_003386"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV3138934.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13229..13738)
                     /locus_tag="RH058_003387"
     CDS             complement(13229..13738)
                     /locus_tag="RH058_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV3138935.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13889..14386)
                     /locus_tag="RH058_003388"
                     /pseudo
     CDS             complement(13889..14386)
                     /locus_tag="RH058_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3466 a         3854 c         3489 g         3577 t
ORIGIN      
        1 tcttgcaccg atatactttt ttcttttctg ttaaaggact ccgttatgtt taaaggacaa
       61 aaaacattgg ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat
      121 gaaggatccg gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat
      181 caggatgata ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag
      241 tcacatcaca gcgatgccgt tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac
      301 tccagcaacg gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg
      361 aaaaccaccg gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc
      421 ggcgtacgtt taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat
      481 atcgacctgg ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa
      541 attgatcagg ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg
      601 gattataaat aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag
      661 gtatggacac cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc
      721 cttgcgcatt ggcggtcgaa ttcaacctta atgttctcga taaatcaatg cgcgaccgca
      781 ttgatatttc attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg
      841 ttgcggtaaa taataaccaa atcagtaacg ggcaaaagat taactggcac aaaaatgacg
      901 ataaaaccat tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag
      961 tccgtcagtc gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc
     1021 tcttcaattt cgatcaagcc aatcagcaac taaatatcac cattccgcaa gcctggctgg
     1081 cgtggcactc agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtgtcc
     1141 tgatggatta caacttgttt gccagcagct accgcccaca ggatggcagc agcagcacta
     1201 atcttaacgc ctatggaaca gccggaatca acacgggggc atggcgctta cgcagtgatt
     1261 atcagttgaa tcagactgat agcgatgata accatgaaca gtcaggcgaa atatcacgta
     1321 cctatctttt tcgcccgtta ccgcaattag gttcaaaatt aaccctcggc gaaacggatt
     1381 ttagttccaa tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgatc
     1441 gaatgttgcc atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca
     1501 atgccacggt gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag
     1561 gcccatttat cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga
     1621 cggaagaaga tggtcgggtg aacaatttcc aggtttcggc agcatcgacg cccttcctga
     1681 ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac
     1741 atcaaactga aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca
     1801 cctcgctgta cggcggcctg ctgctttctg gtgatgacta ccattctgcc gcaataggta
     1861 tcgggcaaaa tatgctgtgg catggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc
     1921 actttgatac tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag
     1981 tggatgccac caacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc
     2041 acagctacgc caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac
     2101 agacgatcag cttatctgtg ggccaaccga ttaccccact aaacctcaat ctttacgcca
     2161 acctgctaca tcaaacctgg tggaatgcag acgcctccac gaccgccaac atcacggcag
     2221 gttttaatgt tgatattggt gactggagag atatctctat ttcgacgtca ttcaatacaa
     2281 cccattacga agataaagat cgcgacaacc agatttacct gtcgatttcg ctccccttcg
     2341 gtaacggtgg tcgggttggc tatgacatgc aaaacagtag ccacagcacc acacaccgca
     2401 tgtcgtggaa cgatacgctg gatgaacgta atagctgggg catgtctgcc ggactgcaat
     2461 ccgaccgtcc ggacaatgga gcccaggtga gcggtaacta tcagcacctg agttcagcgg
     2521 gtgagtggga tatttctggt acctatgccg ccaatgatta cagttccgtc agcagcagct
     2581 ggagcggttc tttcaccgca acccaatatg gtgcagcgtt tcatcgccgc agctccacca
     2641 atgaaccacg cctgatggtc agcaccgatg gcgtggcaga tattccggtt cagggcaatc
     2701 tcgactacac caaccatttt ggcattgcgg tggtgccgtt gatttccagt taccagcctt
     2761 ccaccgtggc ggtgaacatg aatgacttac ccgacggcgt aacagttgca gaaaacgtta
     2821 tcaaagaaac gtggattgaa ggcgcgatag gttacaaatc actggcttcc cgttccggta
     2881 aagacgttaa cgtcatcatt cgcaacgcca gcggtcagtt tcctcccctc ggagcggata
     2941 tccgccagga tgacagcggc attagcgtgg ggatggttgg cgaggaagga catgcctggt
     3001 taagcggagt cgctgaaaat caaaagttta ccgtggtctg gggtgatagc cagcattgct
     3061 cgctccatct tcctgaacat atggaagaca ccgcaaatcg cctgatttta ccttgtcatt
     3121 aattaaggaa atgaaaatga catttatgaa aggactaccc cttttgttgt tggttgccag
     3181 tctgtgcagc cacgctgcac tacaacccga tcgcactcgt attgtgttta acgctaatga
     3241 taaagctacc agtctgcggg tggataatcg tagcgataaa ctcccctacc tcgcttattc
     3301 ctggctcgaa aatgaaaaag gggagaaaag cgatgatctt ctggtcgccc tgccgccgat
     3361 acaacgtctg gaaccgaaag caacaacgca ggtacggata gtaaagcaag cctcgaccac
     3421 aaaattaccg ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc
     3481 cccggaaaaa aacagcgacc atgccgttct gcaagtcgcc attcagagtc gtatcaaggt
     3541 gttctggcga ccggctgcat tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca
     3601 ggtcagccag cagggcaacc aactgacact gaaaaatcct accgcgtatt atctgaccat
     3661 tgcctacctg ggacgaaatg aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc
     3721 gccttttagt acggtcaaca caaacaccgg aaattatagc ggcagccagt tttatctcgg
     3781 ttatatggat gattacggcg cgttacgcat gacgacgctc aactgcagcg gacaatgcca
     3841 tttacaagcc gtggaggcga agaaatgagt gctggcaagg gagtgttgct cgtcatctgt
     3901 ctgttatttc tgccgttgaa gtctgcgctt gccctgaact gctactttgg ttcatccggt
     3961 ggctcggtag aaaaatcaga agcgattcaa ccttttgctg taccaggcaa tgccaaactc
     4021 ggcgataaga tttgggaatc tgacgatatt aaaattcccg tctattgtga caacaatacc
     4081 aacggcaatt ttgaaagtga gcatgtttat gcctgggtaa acccctaccc gggggtgcag
     4141 gatcgctatt atcaactcgg cgtaacttac aatggcgttg attatgacgc caaccaggga
     4201 aaaagccgca tcgacactaa ccagtgtatt gacagtaaga acattgacat ttatacccct
     4261 gagcagatta tcgcgatggg atggcagaac aaaatttgct cgggtgatcc cgctaatatt
     4321 cacatgtcgc gtacctttct cgctcgcatg cggttatacg tcaaaatacg agaaatgccg
     4381 ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg tgcaatttga cggtgccggt
     4441 agcgttaacg aagaccctac tgcccaaaac ctgaaatatc atattactgg tctggaaaac
     4501 attcgcgtgc tggattgcag cgtcaatttt tccatttcac cggaaacaca ggtgattgat
     4561 tttggtaaat ttaatttgct ggatattcgt cgccacacaa tgtcgaaaac attcagtatt
     4621 aagacgacaa aatcacaaaa tgaccaatgc accgacggat ttaaagtcag ttcctccttt
     4681 tataccgaag aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag
     4741 ctacgtttgc tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc
     4801 gatttcacca gcgatatgct gatctacgaa aaaacctata cggctgaact ttcgtccatt
     4861 gcaggccccc ccatcgaggc tggccccttc gatacagtgg tgctttttaa gattaactat
     4921 aactgatttg ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg
     4981 attttcccct tttccttgat tgctgttatt ttttcctggc ccggctttct ttatgctgta
     5041 tcgatacacc aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat
     5101 gttatgtgtg ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc
     5161 gctattactt gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca
     5221 acttccccgc tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa
     5281 cctcactggt tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt
     5341 actggtgacg ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact
     5401 gcctggaaat accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat
     5461 ggcgcaagat tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct
     5521 gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg ctgggtgata acgctgaagc
     5581 ggttaaccag caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt
     5641 gctcgcagtc gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct
     5701 catcccgatg ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc
     5761 ggaatggctg ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga
     5821 taaacaaatc ttactgcggg cactacgccc gctaccgcaa atcctgctgt cgatttttgc
     5881 tctgctggct atttgtgcgg gtatggcgtg ggggctgacc cggtttatgc acattgattt
     5941 tatgactgcc tacctcgcca ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc
     6001 agggagcaat gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat
     6061 tttgctgacg gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc
     6121 ctagtgctgg cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat
     6181 gatgccgcca cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag
     6241 cgccccatga tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat
     6301 ctccagtaac gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg
     6361 atttccagtc aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc
     6421 cagaaacgcc tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa
     6481 taaccgttcc tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg
     6541 ttgtaaaaac ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct
     6601 ataaagcaga atggttttgt cagccgcttg cagttttacc cgcaatactc gcgtggtctg
     6661 cggctcttcg ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag
     6721 cgttaagtcg ttggaaaaat gagttaacaa cgccttacca cgcgtttcca catgggtaac
     6781 gtgttgaccg ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc
     6841 agttagtggt ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg
     6901 gccttcaggc atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca
     6961 acaatctcct tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg
     7021 ccgtcgccat gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt
     7081 ttgactctgc cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca
     7141 atcaacgcgc ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg
     7201 gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt
     7261 cctgccagcc caacgagaat caatgccgga tcgcaagcgt ataccgctct ggcgatggcg
     7321 tctgccagtt gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga
     7381 cgcattacgc cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc
     7441 tgggcgtaaa cggtttctgg cggcaactgc atggcgctgc gaccaaaatt ttccctgtcg
     7501 ggaaaactcg ggtgagcgcc aattgcaaca ccatttttta ttgcttcacg cacgcaagcc
     7561 tgcatggttt gcgcatcgcc tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc
     7621 gttaatagct ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc
     7681 attgtgcagc cgccacgcta attgttcaaa ataacgttgc tgatcttgcc gcgctttcag
     7741 tgcctcttcc agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgcaag
     7801 atggtacata tcagcctcaa tgatgcaggc aatacgcggg taaccaccgg tggtctgtgc
     7861 gtcgttcatc aacacaatcg gctgcccgtt atgtggcacc tgcaccacgc ccggtaacaa
     7921 accgtgagat aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata
     7981 gcccatgcgg ttactttgcg agctaagctg ccagggcgaa cgccagaatg catcctgcga
     8041 ggcacgatcg aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag
     8101 cagctgttta acgccctgcg cttccataaa atcacgcttc gctttgccaa tcgggagtcg
     8161 gtcaccatcc ttcagtaaac ggccttccag cccgccaatc cccactttga gatcggtgct
     8221 gcatgacccc attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat
     8281 cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca atcgccagcc
     8341 ggtccagacg gcattatcat ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca
     8401 cccatcagtt tcgaactcaa cagttaactg accgagcgtg atctccagcg cgggggcatt
     8461 ggcgtcatta cccaccagta ggttagcaat gcgtaacgcg ggcatatcca gtgcgccgca
     8521 gtggctgata cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat
     8581 gcccgcacga ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct
     8641 cccggacgta ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga
     8701 ccaatcaact gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg
     8761 atcccgacag aacctgccgg aacgagtaag cgcggttcag cgcgccgtgg cgtgtgtagt
     8821 tgttccggca aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg
     8881 tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg
     8941 accaccgcca aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga
     9001 gactcaggtt ccagggcctc gctctcctcc caccagcgtt gcaaacgctc tatggcatcc
     9061 agcgccagcg actcaggatt acgcaaaatc accgtaatat tgttcatgcc gggaatggct
     9121 tcaaccacat tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta
     9181 gccagcgtca ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa
     9241 cgcgctcgtt gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat
     9301 aaaggtcaca tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc
     9361 acgttcagtg gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg
     9421 aatggtctgt tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct
     9481 atcgataaaa ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc
     9541 ggtatcgcca caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc
     9601 cagtcccggc acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat
     9661 gaccgtgatc cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc
     9721 aagcggcaaa tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt
     9781 acgcttcatt ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat
     9841 gaccgcatcc gcgaccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc
     9901 ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc
     9961 actaatcgcc gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat
    10021 cttcgcgcct tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg
    10081 tcgatagcca atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt
    10141 ttatatcatc acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat
    10201 aatcagatct gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta
    10261 cgtgacgttg ccgtcaattg tggaattatt atgaataaac acgcatcaca accgcgcgct
    10321 atttactatg tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc
    10381 ctgctgattc tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta
    10441 tttagcgcct actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat
    10501 aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat
    10561 gtggtgctgg gtgccagtga gatccatccg tcattcctct atctgtccct ggcgattatc
    10621 gtttgtggct atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag
    10681 ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt atgcagcggg taacgtgggg
    10741 tctattatcg cacctatcgc ctgtggttac gcccaggaag agtacagctg ggcgatgggc
    10801 tttggcctgg cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt
    10861 catttcacac atacccgcgg cgttaataaa aaagtactgc gtgcaacaaa ctttctcctg
    10921 cctaactggg gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtactg
    10981 ttctggaaag agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta
    11041 ctggcaaaaa tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt
    11101 gtgacgctca ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg
    11161 attagccttt atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact
    11221 gctatgttcc agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg
    11281 gtggttaaag aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt
    11341 ggtctcggcc tgatgagcgc cgggttctgc attctgacct taagcgcccg ctggtccgca
    11401 atgtatggtc actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg
    11461 gaactgttta tcgacccggt tgccatgtcg caaattacgc gcattgaaat tccgggcgtg
    11521 accggcgtat taaccggcat ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg
    11581 ggcgtgattg ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac
    11641 tccatcaatg catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta
    11701 ggactggtac tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg
    11761 ctggagtcat aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct
    11821 cctttcctcg cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact
    11881 atcggttgcg gataatccag cttcacacct gctttctgcg cccacttcca cggctcatgc
    11941 accgctttcc ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca
    12001 cggtcaaatt tctcgccctg agttatcgga ttgaaaatac gaaaatacgg cgcagcatca
    12061 gtacccgtag aagccgccca ctgccagcca ccgttattag ctgccaaatc accatcaatc
    12121 agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga
    12181 aaactggctg taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc
    12241 atagcggcat caacaatcgg gtatcccgtt ttgcctttct gccaggcctg taaatgtgcg
    12301 ggattgcttt gccactgcac acgatccgtc caggcaataa atggacgatg tttacacaac
    12361 gaggggtaat acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag
    12421 acactaccgg ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac
    12481 tggcgaggcg ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact
    12541 gccggaaaat ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat
    12601 tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga
    12661 taattcagcg taatggatgg cgcgggctct atcgatccgc tactacgaac ttttggcgca
    12721 gcgacacact ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta
    12781 aagactttgt acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg
    12841 tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc
    12901 cgctcattca cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac
    12961 acctgtttaa ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga
    13021 ataccttttt ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc
    13081 tgacgcggcg acatgttatg cgtcgcccac tggcgtggtg tagcgatata taacgccagc
    13141 acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg cagacgtaaa
    13201 tcctggcgaa accagaccag atgggtaatc ataaaactcc tgaatggcgc aagttaatca
    13261 cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca
    13321 tatagtgttt gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt
    13381 cgataagcgc cgccaactgc tggcgttctg tggaatcaat atgcgggcga aaataaccct
    13441 gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa
    13501 ttacgcgttg gtagtactgg ttgtaaaaat catccagatt ttgccactgg tgaatatcag
    13561 ccacgaacgg gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat
    13621 agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta
    13681 actcatgaag ggcgaagata cggattaggg tgctgtcatc aaatcgttgc tgattcatta
    13741 ctgctcctga acggtttaca ggagtaagtg tagacgttga gaaatgatat ttgtgaagtt
    13801 aatcctgttg tagcaggaca aatttacgaa agcctgctcc ccgcaaggac tgacgtcagg
    13861 tagtttctaa ccatagctgc cctttctatt caaacgtgaa atcaataagc tcatcaggga
    13921 catcgcaaac aatatgaaga cgggtttctt ctctgccgtg actcttttca ctaattgcgt
    13981 aactgtcatg cttgggatta tttaattctt tcagcggaaa tttttcctcg aaggctttat
    14041 ttagccgccc ctggtttcct tttacagcga ataaataatc acctccctgt ttttgtatct
    14101 tctctgcaat atctttctgg caacccatcg catcggttgt gatgattttt cctttaatat
    14161 ccagcatgtt aagaagttct ggaatagctg tgatctcatt ggatttctta tccgtcttga
    14221 tctgcccgat gaccagactg tgcattgttg agaacgcact aatgacatga atcgctcccc
    14281 tgcggcgact cttgtcataa gagtgccgga gcgtttttcc atcaattgca atgacgtctt
    14341 tatcatttga tgaatggcaa tcacgcatcc agttaataaa gcactc
//
LOCUS       DAPHDX010000111        14210 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPHDX010000111 DAPHDX010000000
VERSION     DAPHDX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.112"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            204..1262
                     /gene="citC"
                     /locus_tag="RH058_003389"
     CDS             204..1262
                     /gene="citC"
                     /locus_tag="RH058_003389"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV3138936.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1277..1573
                     /gene="citD"
                     /locus_tag="RH058_003390"
     CDS             1277..1573
                     /gene="citD"
                     /locus_tag="RH058_003390"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV3138937.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1570..2478
                     /gene="citE"
                     /locus_tag="RH058_003391"
     CDS             1570..2478
                     /gene="citE"
                     /locus_tag="RH058_003391"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV3138938.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2489..4021
                     /gene="citF"
                     /locus_tag="RH058_003392"
     CDS             2489..4021
                     /gene="citF"
                     /locus_tag="RH058_003392"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV3138939.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4025..4576
                     /gene="citX"
                     /locus_tag="RH058_003393"
     CDS             4025..4576
                     /gene="citX"
                     /locus_tag="RH058_003393"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV3138940.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4551..5429
                     /gene="citG"
                     /locus_tag="RH058_003394"
     CDS             4551..5429
                     /gene="citG"
                     /locus_tag="RH058_003394"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV3138941.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5480..6943
                     /gene="citT"
                     /locus_tag="RH058_003395"
     CDS             5480..6943
                     /gene="citT"
                     /locus_tag="RH058_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV3138942.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7057..7863
                     /gene="rna"
                     /locus_tag="RH058_003396"
     CDS             7057..7863
                     /gene="rna"
                     /locus_tag="RH058_003396"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV3138943.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8093..8503
                     /gene="rnk"
                     /locus_tag="RH058_003397"
     CDS             8093..8503
                     /gene="rnk"
                     /locus_tag="RH058_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV3138944.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8734..9972)
                     /locus_tag="RH058_003398"
     CDS             complement(8734..9972)
                     /locus_tag="RH058_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV3138945.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10193..10621
                     /gene="uspG"
                     /locus_tag="RH058_003399"
     CDS             10193..10621
                     /gene="uspG"
                     /locus_tag="RH058_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV3138946.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10742..12307)
                     /gene="ahpF"
                     /locus_tag="RH058_003400"
     CDS             complement(10742..12307)
                     /gene="ahpF"
                     /locus_tag="RH058_003400"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV3138947.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12478..13041)
                     /gene="ahpC"
                     /locus_tag="RH058_003401"
     CDS             complement(12478..13041)
                     /gene="ahpC"
                     /locus_tag="RH058_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV3138948.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13413..14159
                     /gene="dsbG"
                     /locus_tag="RH058_003402"
     CDS             13413..14159
                     /gene="dsbG"
                     /locus_tag="RH058_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV3138949.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3379 a         3759 c         3579 g         3493 t
ORIGIN      
        1 aaataaaaaa ccactaactc tatgtgaaat aaatcaaaat ttcacgccga aatactcctt
       61 aggatgtata gcgaaaggag aaaaagatat acctcgatca cccccctttc tcccaagtga
      121 aaataaaagg ttatcagttt gcaacattga acaacattcg ttgcaaatcg ataacaacat
      181 gcaccttcag gatactattt attatgttcg gcaatgatat tttcacccgc gtaaaacgtt
      241 cagaaaataa aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg
      301 acaccacagt cgaagtattt attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg
      361 gaattgccgg aaatattatt aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac
      421 tggcgctgac attagccact gaactgataa acctcgccta tgagcggcac agcacgcatc
      481 tgtttattta taccaaaacc gaatacgagg cgctgttccg ccagtgcggt ttttccacgc
      541 tgacctgcgt acccggcgtg atggtgctga tggaaaacag cgccacgcga ctgaaacgct
      601 atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc attgtgatga
      661 acgccaatcc ctttacgaat ggtcaccgtt atctgattca acaagctgcg gcacagtgcg
      721 actggttgca tctgttttta gtcaaagaag attcttcacg cttcccctat gaagaccggc
      781 tggatctggt gttaaaaggc accgccgata ttccacgcct gactgtgcat cgcggctccg
      841 aatacatcat ctcccgcgct acgttccctt gctacttcat taaagaacag agcgtcatta
      901 accattgtta caccgaaatt gatcttaaga ttttccgtca gtacctcgct cccgcgctgg
      961 gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc
     1021 aggatatgcg ctactggctg gaaacgccga ctatctccgc accgcccatc gaactggttg
     1081 aaattgagcg gctgcgttac caggagatgc cgatatccgc ttcccgggta cgtcaactgc
     1141 tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt
     1201 tgcagaacct gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag acccccgcat
     1261 gagaaacagg tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc ttgagtctgg
     1321 ggatgtgatg atacgcatcg ccccactcga tacgcaggat atcgacctgc aaatcaatag
     1381 cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc attctggacg ttctcgcccg
     1441 ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa ggcgcactgg actgcatttt
     1501 acgtgcacga ctggaagccc tgctggcacg cgccagcggt atcccggctc tgccatggga
     1561 ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg ccgcagcatg
     1621 ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta cccggctgat
     1681 gccctgatgt ttgacctcga agactccgta gcattgcgtg aaaaagacac cgcccgccgc
     1741 atggtttacc acgcgctgca acatccgctg tatcgcgata ttgaaaccat tgtgcgtgtc
     1801 aacgcgctgg attccgaatg gggtgtcaac gacctggaag ccgtcgttcg cggtggtgcg
     1861 gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg ttctggatat cgaaaaagag
     1921 atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca gcaccggcct gctggcggcg
     1981 attgaatctc cgctgggcat tacccgcgca gtggaaatcg ctcacgcttc cgagcgtttg
     2041 atcggtatcg ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga acgctccccg
     2101 gaaggaactg aactgctgtt cgcacgctgt tccattttgc aggccgcgcg ctctgcgggt
     2161 attcaggcgt tcgataccgt ctattccgac gctaacaacg aagccggatt cctgcaagaa
     2221 gccgcccaca tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc gcgtcagatt
     2281 gatctgttgc acaacctcta cgcaccaacc cagaaagaag tggatcacgc ccgccgcgtc
     2341 gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct gaacggcaag
     2401 atggtggacg gtccggttat cgatcgcgcc cgtctggtgc tctctcgtgc agaactttcc
     2461 ggcatccgcg aagaataagg caatcaaaat gacgcagaaa attgaacaat ctcaacgaca
     2521 agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat cttgccgctt tccagaactc
     2581 gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc aaactgtgcg ccaacctgga
     2641 agaagcgatt cgtcgctctg gtttacagga cggcatgacg gtttccttcc atcacgcttt
     2701 ccgtggcggt gacctgaccg tcaatatggt gatggacgtc atcgcgaaga tgggctttaa
     2761 aaacctgacc ctggcgtcca gctccctgag tgattgccat gcgccgttgg tagaacacat
     2821 tcgccagggc gtggttaccc gcatttatac ctccggcctg cgcggtccac tggcggaaga
     2881 gatctcccgt ggtctgctgg cagaaccggt gcaaatccac tctcacggcg gtcgcgtgca
     2941 tctggtacag agcggcgaac tgaatatcga cgtggctttc ctcggcgtcc cgtcctgcga
     3001 tgaattcggt aatgccaacg gctacaccgg taaagcctgc tgcggctccc tcggctatgc
     3061 aatggttgat gccgacaacg caaagcaggt cgtgatgctt accgaagaac tgctgcctta
     3121 tccgcataat ccggcaagca ttgagcaaga tcaggttgat ttgatcgtca aagttgaccg
     3181 cgttggcgat gctgcaaaaa tcggcgctgg cgcgacccgt atgaccacta acccgcgcga
     3241 actgcttatt gcccgtagcg ctgcggatgt gattgtcaac tcaggctact tcaaagaagg
     3301 tttctccatg caaaccggca ccggcggcgc atcgctggcg gtaacccgtt tcctggaaga
     3361 caaaatgcgt agccgcgata ttcgcgccga cttcgccctt ggcggtatta ccgcgacgat
     3421 ggttgacctg cacgaaaaag gtctgatccg caaactgctg gatgtgcaga gctttgacag
     3481 ccatgctgcg caatcgctgg cccgtaaccc caatcacatc gaaatcagcg ccaaccagta
     3541 cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc gacgtggtgg tactgagcgc
     3601 gctggaaatt gacacccagt tcaacgttaa cgtgctgacc ggctctgacg gcgtactgcg
     3661 tggtgcttcc ggtggtcact gcgataccgc gattgcctct gcgctttcca tcatcgtcgc
     3721 gccgctggta cgcggtcgta ttccgactct ggtggataac gtgctgacct gcatcacccc
     3781 gggctccagt gtcgatattc tggtcacaga ccacggtatc gcagttaacc cggcacgtcc
     3841 ggaactggca gaacgtctgc aggaagcggg cattaaagtg gtttccattg agtggctgcg
     3901 cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt gaattcaccg accgcgtcgt
     3961 tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt gtgcatcagg tgaaggaata
     4021 agccatgcac ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc ccgagctgct
     4081 cgtcagccgg gatgaaaggc aagcacggca acacgtctgg ctcaagcgcc atcctgttcc
     4141 actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa gacagcgagg tcacacgccg
     4201 aatttttaat catggcgtga cagccttgcg tgccttagcc gcaaaacagg gctggcaaat
     4261 tcaggagcag gctgcactgg tttccgccag cgggccggag ggcatgttga gcattgccgc
     4321 cccggctcgc gacctcaagc tcgccaccat tgagcttgaa catagtcatc ctctcgggcg
     4381 gttatgggat atcgatgtcc tgacgcccga aggcgaaatt ctctcccgcc gcgactattc
     4441 actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca gccgtctgcg cgcgtggaaa
     4501 aacccatcaa ctgaccgatt tactcaaccg catggaggca ctgctgaacg atgtcgatgc
     4561 ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt aatcgatgag tacgccctgc
     4621 tgggctggcg cgccatgctg actgaagtca atctgtcacc gaaaccaggc ctcgtggatc
     4681 gcattaactg cggtgcgcac aaagatatgg cgctggaaga tttccaccgc agcgcgctgg
     4741 cgattcaggg ctggttaccc cgtttcattg aatttggtgc ctgtagtgca gaaatggcac
     4801 cggaagcggt actccacgga ttacgcccaa ttggtatggc ttgcgaaggt gatatgttcc
     4861 gcgccactgc gggcgtaaac acgcataaag gcagcatttt ttctttaggg ctgctatgtg
     4921 cggcaattgg ccgtttgctt cagctcaacc agccggtaac gccaacaacc gtttgttcta
     4981 cggcggcaag tttctgccgt ggtctgaccg atcgcgaact gcgtaccaat aattcacaac
     5041 tgacggcagg tcaacggttg taccagcagc ttggccttac cggcgcacgc ggtgaagccg
     5101 aagcgggtta tccactggtg atcaaccacg ccttgccgca ttacctcact ctgctggatc
     5161 aggggttaga tcctgaactg gcattgctcg ataccttgct cctactgatg gcgatcaacg
     5221 gcgataccaa cgttgcttcg cgcggtggcg aggggggcct gcgctggcta cagcgcgagg
     5281 cgcaaacatt attgcaaaaa gggggcattc gaacccccgc cgatctcgat tatctccggc
     5341 agttcgacag ggagtgtatc gaacgaaatc tcagtccagg cggcagtgcc gacctgctga
     5401 tccttacctg gtttttagca cagatttaat tatttaagca cttgataaat ttgaaaatat
     5461 taattttcgg agaacccgta tgtctttagc aaaagataat atatggaaac tattggcccc
     5521 actggtggtg atgggtgtca tgtttcttat ccctgtcccc gacggtatgc caccacaggc
     5581 atggcattac ttcgctgtgt ttgtggcaat gattgtcggc atgatcctcg agccaattcc
     5641 ggcaacagcg atcagtttta ttgcggttac tatttgcgtt attggcagta attacctgct
     5701 ctttgatgcc aaagaattag ctgacccagc gtttaatgcg caaaaacagg cgctgaaatg
     5761 gggtctggct ggtttttcca gcaccacggt atggctggta tttggcgcat ttatttttgc
     5821 attagggtat gaagtttccg ggttaggtcg tcgcattgcc cttttcctgg tgaaattcat
     5881 gggcaaacgc acgctgacgt tgggttatgc gattgtcatt atcgacattc tgctggcacc
     5941 gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt tttccggtca ttaaaaacct
     6001 gccgccgttg tttaaatcat tccccaacga tccgtccgcg cgtcgtattg gcggctattt
     6061 gatgtggatg atggtcatta gtaccagtct gagttcgtcc atgtttgtca ccggtgcggc
     6121 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6181 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6241 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6301 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6361 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6421 tctgctggca gtttcgctaa tgctggccct gcatgttgtg ccgtggaaag acattacccg
     6481 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6541 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacac acctggaagg
     6601 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6661 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6721 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     6781 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     6841 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt atatctctat
     6901 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     6961 gaataaatca tcacgcccgc caggttagcc cacctggcgg gcttttttat gatttaatag
     7021 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg gcgtaacgcc
     7081 gcgttgctcg cggtttctct gcttcccttc tcttctgcca acgccgtagc gttgcaggca
     7141 aaacagtatg gcgactttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7201 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7261 accaacaaag ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7321 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7381 ccgaatctac cagaagcgcg cgccagccgg atgtgttcat cgccggaaac cggattatca
     7441 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7501 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7561 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7621 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7681 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7741 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     7801 ttgccacaac cacacccagg caactgtggc aaaacctttg tgattgataa agtgggttat
     7861 taatcatctg atttacaaaa cacgctaatt cgtcttccat aaaccgtgac gattaagaat
     7921 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     7981 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8041 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8101 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8161 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8221 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8281 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8341 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8401 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8461 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8521 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8581 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8641 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8701 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     8761 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     8821 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     8881 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     8941 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9001 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9061 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9121 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9181 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9241 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9301 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9361 ggcgattgtc aacaatccca ctggaccggc accatagact gcaacgcttg aaccttgttg
     9421 gatctgcgca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9481 tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt tagggacgcg
     9541 gacatattcc gcctgtccac caggaacgcc accatacagg tgactataac caaacaatgc
     9601 cgcgggagct ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9661 gcaggcggca tattgctgca ttcgacagaa aaaacagtcg ccacaagcaa tgacaaacgg
     9721 aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc tttccggttt caacgacttc
     9781 ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata
     9841 aagatggagg tcagagccac agatcgccgt tgccgtaata cgcagaataa tatcatctgc
     9901 ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt
     9961 caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct
    10021 atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctggt gttcagtgag
    10081 aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa
    10141 atattataat catggcgaat gatttgttca tgattaacag ggagaaaggg ttatgtataa
    10201 gacaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca
    10261 cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc
    10321 agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca
    10381 acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg
    10441 cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga
    10501 actgggggct gatgttgtag ttattggttc tcgcaaccca tcgatttcga cccatctgtt
    10561 aggttctaac gcctcgagcg tgatccgcca tgccaatctg ccggtgctgg ttgtgcgtta
    10621 attgccagaa taaatatccc gccctgcccg gagccatccc ggcagggctt tttaatgcca
    10681 taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt
    10741 cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt
    10801 cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaata
    10861 cgcctttgac gttggtttcg catttcgcat caatgataat ctcacccatg cggttacgtt
    10921 cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggacg aaaataccgg
    10981 ccagttcgat gttgtgaata tcgccgctga cacgatctcg atattccaga ccaacgactt
    11041 tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca
    11101 ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca aattcgagca
    11161 gcgttacgtg ctcaacgata cccgccaggt caattgccgc ttccacgccg gagttaccgc
    11221 cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg
    11281 tcacgccttt ggtgcgatac tgatcttcgc caggaacgtt catgttgcgc cattttgcac
    11341 cggtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctgat
    11401 gcagaccacc ttcaactgct gctgggatca gtttgctggc gctctggctg tcgatcacat
    11461 caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc cagcttctgc ccttcagtct
    11521 tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt
    11581 cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg
    11641 ccgggccgga accgacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg
    11701 cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct
    11761 gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga
    11821 tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcagtacgc
    11881 tcatcaggtt cagcgcctgc accacgtccg ggcagttgtg gcaagagagc gagtaatagg
    11941 tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt
    12001 tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac tcgtggccca
    12061 gcggggagcc tgcaaaacgt ggcccctggt tggaacctgg gttggtgatc aggaaagacg
    12121 gcttacgcac cggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga
    12181 tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc gtggcaatta
    12241 actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt
    12301 cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct
    12361 ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt
    12421 aggtgaattt ccgaagtatt tcgcgccgga gttggtagcg cgaaagacga aggaaattta
    12481 gattttacca accaggtcca gagacggagc cagagttgct tcaccttctt tccatttagc
    12541 cgggcaaact tcacctgggt gagaagctac gtactgtgct gctttgattt tacgcagcag
    12601 gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg attgcctgga tgataccctg
    12661 cgggtcaaca acgaaggtcg cacggtcagc cagaccttca tcttcacgca tgttgtcgaa
    12721 gttacgggtc agggcgccag tcgggtcgcc gatcatcgca tatttgattt tagcgatggt
    12781 ttcagagctg ctgtgccatg ctttgtgggt gaagtgagta tcggtagata ctgcgtatac
    12841 gtctacgccc agtttctgca gttcttcgta gtggtcagca acgtcaccca gttcggtcgg
    12901 gcatacgaaa gtaaagtcag ccgggtagaa gaagaagacg ctccagcggc cttcggtatc
    12961 tttttcggtg atttcgatga attcgccgtt tttgaatgcc tggtttttaa aaggtttaat
    13021 tttggtgtta atcaaggaca tctatacttc ctccgtgttt tcgatgagat gtaaggtaac
    13081 caatttttgc cgattcggct aatgcgtttc cattatcaaa tcgataagtt ttaccttaca
    13141 accttcgtaa gacaactttg tgaactttgc accgccagcg gcgataaaaa gtaaaatggc
    13201 cccctttcca ggtgaccgtg taatctgaac taccctttgg tatcttcagt gcctgcctgc
    13261 aggctgaggt gttgccctta caaatgcaac aacgacatgg attacaacac cctcataaac
    13321 aaagggcaat cacctgatct aagctcttac ctatgacagt gataggttat gccttttact
    13381 cgacttttgc actgactgaa aaggacaaat taatgttaaa aaagatactt ttactggctc
    13441 tgcttcctgc aatcgccttc gcagaggaac ttcctgctcc agtaaaagcg attgaaaaac
    13501 agggcattac aatcatcaaa acattcgatg cccccggagg aatgaaaggt tatctcggaa
    13561 agtatcagga tatgggcgtc accatctacc tgactccaga tggtaagcac gctatctctg
    13621 gttacatgta caacgagaaa ggtgaaaacc tgagtaacac acttatcgaa aaagaaattt
    13681 acgcaccagc cggacgcgaa atgtggcaac ggatggaaca atcccactgg ctcctcgacg
    13741 gtaaaaaaga tgcgccggtc attgtctacg tgttcgccga tccgttctgc ccatattgta
    13801 aacagttctg gcagcaggcg cgcccgtggg tagattctgg caaagtgcaa ttaagaacat
    13861 tgttggttgg ggttatcaag ccagaaagcc cggcgacagc agcggcaatt cttgcctcca
    13921 aagatcccgc aaaaacctgg caagaatatg aatcctctgg tggcaagctt aagctaaacg
    13981 tgcctgcaaa cgtaagtaca gaacaaatga aagtgttaag cgccaacgag aaactcatgg
    14041 acgatctggg ggcaaatgtc acaccagcga tctattacat gagtaaggaa aatacgctgc
    14101 aacaggccgt ggggttgccc gatcagaaaa cgcttaatat cattatgggg aataaataaa
    14161 agataattca tggatggttg aaatacatcc ctgcttttca accatcctaa
//
LOCUS       DAPHDX010000112        14146 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPHDX010000112 DAPHDX010000000
VERSION     DAPHDX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.113"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(225..1109)
                     /gene="yghA"
                     /locus_tag="RH058_003403"
     CDS             complement(225..1109)
                     /gene="yghA"
                     /locus_tag="RH058_003403"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV3138950.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            1300..1794
                     /locus_tag="RH058_003404"
     CDS             1300..1794
                     /locus_tag="RH058_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV3138951.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(1834..2874)
                     /gene="gpr"
                     /locus_tag="RH058_003405"
     CDS             complement(1834..2874)
                     /gene="gpr"
                     /locus_tag="RH058_003405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV3138952.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            3031..3918
                     /locus_tag="RH058_003406"
     CDS             3031..3918
                     /locus_tag="RH058_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV3138953.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     ANIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            4037..4324
                     /locus_tag="RH058_003407"
     CDS             4037..4324
                     /locus_tag="RH058_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV3138954.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            4513..5631
                     /gene="hybO"
                     /locus_tag="RH058_003408"
     CDS             4513..5631
                     /gene="hybO"
                     /locus_tag="RH058_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV3138955.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            5634..6620
                     /gene="hybA"
                     /locus_tag="RH058_003409"
     CDS             5634..6620
                     /gene="hybA"
                     /locus_tag="RH058_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV3138956.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            6610..7788
                     /gene="hybB"
                     /locus_tag="RH058_003410"
     CDS             6610..7788
                     /gene="hybB"
                     /locus_tag="RH058_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV3138957.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            7785..9488
                     /gene="hybC"
                     /locus_tag="RH058_003411"
     CDS             7785..9488
                     /gene="hybC"
                     /locus_tag="RH058_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV3138958.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            9488..9982
                     /locus_tag="RH058_003412"
     CDS             9488..9982
                     /locus_tag="RH058_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV3138959.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            9975..10463
                     /gene="hybE"
                     /locus_tag="RH058_003413"
     CDS             9975..10463
                     /gene="hybE"
                     /locus_tag="RH058_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV3138960.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            10456..10797
                     /gene="hypA"
                     /locus_tag="RH058_003414"
     CDS             10456..10797
                     /gene="hypA"
                     /locus_tag="RH058_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV3138961.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            10810..11058
                     /gene="hybG"
                     /locus_tag="RH058_003415"
     CDS             10810..11058
                     /gene="hybG"
                     /locus_tag="RH058_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV3138962.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(11181..12047)
                     /gene="yghU"
                     /locus_tag="RH058_003416"
     CDS             complement(11181..12047)
                     /gene="yghU"
                     /locus_tag="RH058_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV3138963.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKHTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            12252..14111
                     /gene="gss"
                     /locus_tag="RH058_003417"
     CDS             12252..14111
                     /gene="gss"
                     /locus_tag="RH058_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV3138964.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKADGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
BASE COUNT         3267 a         3565 c         3848 g         3466 t
ORIGIN      
        1 gcggaaggga tgaaaaaaac agcgcctgac ggcgctgtgt ctggcatgcc tgcaatccgg
       61 gaaaccggac caggaaaaaa cttgcaggcc ataacagtat ctacaaaatc tattgcaaca
      121 tgttgaatct tcatgcgttt gtaggcggat aaggcgtttt cgccacatca ggtaagagtg
      181 aatttacaat gatgcccggt tgcttttcac aaccgggcat ttttttaacc taaatgctcg
      241 ccgccgcaca caccgtgcac ttctgcggtg acgtagctcg actcctgact tgccagataa
      301 acatatacag gggccagttc cgccggttgc cccgcacgct tcatcggcgt tttctgacca
      361 aactgcggga tcttatcctg cgtttgtccg ccggaaattt gcagtgccgt ccagataggg
      421 cctggcgcga caatattcac ccgaatacct ttctccgcga cctgttttgc caggccacgg
      481 ctgtagttca gaatcgccgc cttcgtagcc gcatagtcca gtaaatgcgg acttggctgg
      541 tatgcctgga ttgacgaagt ggtgatgata cttgcacctt tcggtagcag ggggatcgct
      601 tcctgggtta gccagaacag cgcgaaaacg ttaatggcaa aggtcttttg aaactgttcg
      661 ctggtgaggt ctgcaatatc cggaatggca acctgtttcc cggcgaccag cgccataata
      721 tccagcccgc ctaacgcctt atgcgcttcg tgaaccagcg aacgggcgaa tttctcatcg
      781 cttaaatcgc ctggcagcag aacggctttg cgtccgcatt cttcaatgat ctttttcaca
      841 tcctgagcgt cttcttcttc cacgggaaga taactgatcg ccacgtcagc cccttcacgc
      901 gcgtaagcga tggcggcagc gcgacctatt ccggaatcgc cccctgtcac cagtgcttta
      961 cgatctttca ggcgaccgct accaacatag gttttctcgc cgcaatccgg taccggtgtc
     1021 atcttcgcct ggatgcctgg cgtcggttgt ttctgtttgg gatattcacc agtgtaatac
     1081 tgcgtggtcg ggtcttttaa atgagacatc gtttttctcc cttcaggttc aacgtccttt
     1141 aagggtagac gctctcgatg cgttgataag ggaaccagga agatccctaa acctcagaat
     1201 tatgcgacaa aggtttaacg tatatgttga tttgctgttg cgtgctgttt actcaattgc
     1261 gatatactgt tgcccgtttt aactacacga caggaatgta tggaacgttt tcttgaaaat
     1321 gcaatgtatg cttctcgctg gctgcttgcc cccgtgtact ttggcctttc gctggcgtta
     1381 gttgccctgg cgctgaagtt cttccaggag attattcacg tactgccgaa tatcttctcg
     1441 atggcggaat cagatttgat cctcgtgttg ctgtcgctgg tggatatgac gctggttggc
     1501 ggtttgctgg tgatggtgat gttttccggt tatgagaatt tcgtctcaca gctggatatc
     1561 tccgagaaca aagagaagct gaactggctg gggaaaatgg acgcaacgtc gctgaaaaac
     1621 aaagtagcag cgtcgattgt ggcaatttct tccattcacc tactgcgcgt ctttatggat
     1681 gcgaaaaatg tcccggataa caaactgatg tggtacgtca ttatccatct gacgtttgtg
     1741 ctttctgcat ttgtgatggg ctatcttgac cgactgactc gtcataatca ctgatctgat
     1801 tcgggcgcgg ttcgcgcgcc cgttattaac aggtcattta tcggacgacg cctgccacag
     1861 attcagctcg ccatcggcga tatgctgatc aatctgcgcc agctcctcgg tgctaaatgt
     1921 cagattattc agcgcctgca cgttctcctc aagttgctcc gcgcggctgg caccaatcaa
     1981 taccgacgtc actcgctcat ctttcagcaa ccaacttaac gccatttgcg ccattgattg
     2041 tccacgctgc tgtgccattt cattcaataa gcgcaggctg ttgaggttgg cttcggtaag
     2101 cattttcggt gtcagaccac gaactttatt cccttcacga tacatccgtg aatcttgcgg
     2161 aatgccgttg agatattttc cggtcagcaa tccctgagcc agaggagtaa aggcaataca
     2221 gcccacgccg ttattttgca gggtatccag caggccgctt ttatccaccc agcggttcag
     2281 taaattgtac gaaggttgat gaattaaaag cggaattttc cactcgtgca gcaactcgac
     2341 cattttttgc gtccgctctg gcgagtaaga ggagatcccg acatacagcg ccttaccgct
     2401 ttgtaccgca tgagccagcg cagaggcggt ttcttccatc ggcgtatttt catcgacgcg
     2461 atgagagtaa aagatatcga catactcaag ccccatacgc ttcaggcttt ggtcgaggct
     2521 ggcgagcagg tatttacgtg aaccgccaga gccgtaaggg ccgggccaca tatcgtagcc
     2581 agccttggta gagataatca gttcatcgcg ataagcggca aaatcctccc gcagcaggcg
     2641 accaaagttc tcttctgcgc ttcctggagg cggcccgtaa ttgttggcta aatcaaagtg
     2701 cgtaatgccc aaatcaaacg ctttacgcag gatcgcacgc tgtgattcca gcgcgttaac
     2761 gtgaccgaaa ttgtgccata aaccgagcga taacgcgggc aggcgtaaac cgctttttcc
     2821 gcaatagcgg tattgcatct gcccgtaacg ttcgggattc gctaaccaga ccatgacctc
     2881 tcctttccac cgttcaattt cgaaacaatg tttctagttt agcgattcgc cagcgcgtat
     2941 cccgtagtct ggctcacaga gtgacgaaaa actgggcaaa aacacgtgct tatgctttgc
     3001 ttaaaaaaac accagttgag gagtgcaacg atgccgcgtt taaccgccaa agatttccca
     3061 caagagttgt tggattacta cgactattac gctcacggga aaatctcgaa acgtgagttc
     3121 ctcaaccttg cggcgaagta tgcggtgggc gggatgacgg cattagcgtt gtttgatttg
     3181 ctcaagccaa attatgcgct ggcgactcag gtagagttta ccgacccgga gattgttgct
     3241 gagtacatca cgtatccttc gccaaatggt cacggcgagg tacggggtta tctggtgaaa
     3301 cccgcaaaaa tgagcggcaa aacgccagcc gtggtagtgg tgcatgagaa tcgtggactg
     3361 aatccgtata tcgaagatgt ggcacggcga gtggcgaagg cggggtatat cgccctggca
     3421 cctgacggct taagttccgt tggaggttat ccgggaaatg atgataaagg tcgtgagctg
     3481 caacagcagg ttgatccaac caaactgatg aatgatttct ttgccgcaat tgagtttatg
     3541 caacgctatc cgcaagcggc aggcaaagtg ggtattaccg gattttgcta tggcggtggc
     3601 gtatcgaacg cggcggctgt cgcgtatccg gaactggcct gcgcggtgcc gttttatggt
     3661 cgtcaggcac ccactgccga tgtggcgaat attgaagcgc ctttactact ccacttcgcg
     3721 gaactggaca cccgaatcaa cgagggctgg cctgcttacg aggcggcgtt gaaagccaat
     3781 aataaggttt atgaggcgta tatctatccg ggggttaatc acggattcca taatgattcc
     3841 acgccccgtt atgacaaatc tgccgccgat cttgcctggc aaaggacact gaaatggttc
     3901 gataaatatc tctcctgata ggtttatctc ttactggatt acgtcttaaa caagcatgaa
     3961 aaaatagcgt gcgcaaaagt cgttctttgc ctaaaatatc gctatatatg gcgatatata
     4021 gcgaatgagg tgaacgatga ataaccattt tggtaaaggc ttaatggcgg gattaaaagc
     4081 aacgcatgcc gacagtgcgg ttaatgtgac aaaattctgt gccgattata aacgcggttt
     4141 tgtattaggc tactcacacc ggatgtacga aaagaccgga gatcgccagc ttagcgcctg
     4201 ggaagcgggt attctgacgc gccgctatgg actggataaa gagatggtaa tggatttctt
     4261 tcgtgagaat aattcctgtt ctacgttgcg cttttttatg gccggttatc gcctcgaaaa
     4321 ttgatcaaac atacgtatta tcttgcttta attaattaca ctaatgcttc ttcccttcgt
     4381 tttagcgccc cgccgcagta tcatgatatc gataaccata ataaatgtgt ggtaaatggc
     4441 gcatcgatcg cattattgat tttgcgattg aggcaaaata tatgccaggt cttcgcaacg
     4501 gaataactat aaatgactgg agataacacc ctcatccatt ctcacggcat taaccgtcgt
     4561 gatttcatga agctttgtgc agcattagcc gccaccatgg ggttaagtag caaagccgct
     4621 gcagagatgg ccgaatcggt tactaacccg cagcgtccgc cagttatctg gattggcgcg
     4681 caggagtgca ccggttgtac ggaatctctg cttcgtgcaa cgcatccaac ggtagaaaac
     4741 ctcgtgctgg agactatctc tctggagtat cacgaagtgc tttccgccgc cttcggtcat
     4801 caggtcgaag agaacaaaca taacgcactt gagaagtaca aagggcagta tgtgttggtt
     4861 gtggatggtt ccatcccatt aaaagataac ggtatttatt gcatggttgc tggtgagccg
     4921 attgtggatc acatccgcaa agcggcagaa ggcgcagcag cgattatcgc tatcggttcc
     4981 tgctctgcgt ggggcggtgt tgccgcagct ggagttaacc caactggcgc agtcagcctg
     5041 caagaagttc tgccaggcaa aaccgttatc aatattccgg gctgcccgcc gaacccgcac
     5101 aacttcctcg ctaccgttgc gcacatcatc acttacggca aaccgccgaa actggatgac
     5161 aaaaatcgtc cgaccttcgc ctatggccgt ctgattcacg aacactgcga acgtcgcccg
     5221 cacttcgatg ctggtcgttt tgccaaagag ttcggtgatg aaggccaccg tgaaggctgg
     5281 tgcctgtacc acctcggctg taaagggcca gaaacttacg gcaactgctc aacgctgcaa
     5341 ttctgcgatg ttggcggtgt gtggccggtg gcgattggtc acccgtgcta tggctgtaac
     5401 gaagaaggta tcggcttcca taaaggcatc catcagcttg ccaacgtcga aaatcaaact
     5461 ccgcgttcac agaaaccgga tgttaacgct aaagagggtg gcaacgtctc tgcaggcgct
     5521 attggtttgc tcggcggtgt ggttgggttg gttgccggtg tcagcgtgat ggcggtgcgt
     5581 gaactgggtc gtcagcaaaa gaaagataac gctgactcac ggggagaata accgtgaaca
     5641 gacgtaattt tattaaagca gcctcctgcg gggcattgct gacgggcgcg ctgccgtctg
     5701 tcagtcatgc ggctgctgaa aaccgcccgc caattccggg atcgctgggg atgttgtacg
     5761 actcgacctt gtgcgtaggc tgccaggctt gcgtcaccaa gtgtcaggat atcaatttcc
     5821 ctgaacgtaa cccgcaaggg gaacagacct ggtcgaacaa cgacaaactg tcgccgtata
     5881 ccaataacat cattcaggtg tggaccagcg gcacaggggt caacaaagac caggaggaga
     5941 acggctacgc gtacattaag aaacagtgta tgcactgcgt cgatccgaac tgtgtctctg
     6001 tgtgcccggt ctctgcactg aaaaaagatc cgaaaaccgg cattgtccat tacgacaaag
     6061 acgtgtgcac cggttgccgt tactgcatgg tcgcctgtcc gtacaacgtg ccgaagtacg
     6121 actacaacaa cccgtttggt gcgctgcata agtgcgagct gtgcaaccag aaaggtgtgg
     6181 aacgtctcga taaaggcggt ctgcctggct gcgtagaagt gtgcccggcg ggcgcggtga
     6241 ttttcggtac gcgtgaagag ctgatggcgg aggcgaaaaa acgtctggcg ctgaagcctg
     6301 gcagcgaata ccactatccg cgtcagacgc tgaaatctgg cgacacttac ctgcatacgg
     6361 tgccgaaata ttatccgcat ctgtacggcg agaaagaggg cggcggtact caggttctgg
     6421 tactgacggg tgtgccttat gaaaatctcg acctgccgaa actggacgat ctttctaccg
     6481 gtgcgcgttc cgaaaatatt caacacaccc tgtataaagg catgatgcta ccactggctg
     6541 tgctggcggg cttaaccgtg ctggttcgtc gcaacaccaa aaacgaccat cacgacggag
     6601 gagacgatca tgagtcatga tccaaaaccg ctgggcggca aaatcatcag taaaccggtc
     6661 atgatttttg gaccgttaat cgtcatctgt atgctcctga ttgtgaagcg tctggtgttc
     6721 ggtctgggct ctgtctccga cctgaacggc ggctttccgt ggggcgtgtg gatcgcgttt
     6781 gacctgctga tcggcactgg ctttgcctgt ggcggctggg cgctggcgtg ggcggtatac
     6841 gtctttaacc gtgggcaata ccatccgctg gtgcgtccgg cgctgttggc gagtctgttt
     6901 ggttactcac tgggtggctt gtcgatcact atcgacgtgg gtcgctactg gaacctgccg
     6961 tacttctaca ttccgggtca cttcaacgtg aactcggtac tgttcgagac ggcggtctgt
     7021 atgaccatct acatcggcgt gatggctctg gagtttgctc cggcactgtt tgaacgtctg
     7081 ggctggaagg tgtcgctaca gcgtctgaac aaggtgatgt tcttcatcat cgcgctcggt
     7141 gcgttgctgc cgaccatgca ccagtcttca atggggtcgc tgatgatctc ggcgggctac
     7201 aaggtgcatc cgctgtggca gagctatgaa atgttgccgc tgttctcgct gctgacggcg
     7261 ttcatcatgg gcttctcgat tgtcatcttt gaaggttcac tggtgcaggc gggtctgcgt
     7321 ggcaacggtc cggatgaaaa gagcctgttc gtcaagctga ccaacaccat cagtgtgttg
     7381 ctggcgattt tcatcgtgct gcgctttggc gagcttatct atcgcgacaa gctgtcgtta
     7441 gcgtttgccg gtgacttcta ctccgtgatg ttctggattg aagtcctgct gatgctcttc
     7501 ccgctggtcg ttctgcgtgt ggcgaagctg cgtaatgatt cccgcatgct gttcctgtca
     7561 gcactgagcg cactgttagg ttgtgcaacc tggcgtctga cctattcgct ggtggcattc
     7621 aacccgggcg gcggttacgc ctacttcccg acctgggaag aactgttgat ttctattggt
     7681 tttgtggcta ttgagatttg cgcttacatc gtactcattc gtctactgcc gatacttcct
     7741 cctttaaaac aaaacgatca taatcgtcat gaggcgagca aagcatgagc cagagaatta
     7801 ctattgatcc ggtaacccgt attgaggggc atttacgcat cgattgcgaa atcgaaaatg
     7861 gcgtcgtttc gaaagcatgg gcttccggta ccatgtggcg cggcatggaa gagatcgtga
     7921 aaaaccgcga tccgcgcgat gcatggatga ttgtgcaacg tatctgtggc gtatgtacta
     7981 ccactcacgc gctgtcttcc gttcgtgcgg cagaaagtgc gctgaatatc gacgttccgg
     8041 ttaacgcgca atacatccgt aacatcattc tggctgcgca caccacgcat gaccatatcg
     8101 ttcatttcta tcagctttcg gcgctggact gggtggatat cacttctgca ctgcaagctg
     8161 acccaaccaa agcctccgaa atgctgaaag gcgtttcgac ctggcatctg aacagcccgg
     8221 aagagttcac caaagttcag aacaagatca aagatctggt tgccagcggt cagttgggta
     8281 ttttcgctaa tggctactgg ggtcacccag cgatgaaact gccgccggaa gtgaacctga
     8341 ttgcggtagc gcactacctg caggcgctgg agtgccagcg tgacgctaac cgcgtcgtgg
     8401 cgctgctggg cggtaaaacg ccgcacattc agaacctggc agtgggtggt gtcgcgaacc
     8461 caatcaacct cgacgggttg ggcgtgttga accttgagcg cctgatgtac atcaagtctt
     8521 tcatcgacaa actgagcgac tttgttgagc aggtttataa ggttgatacc gcggttatcg
     8581 ccgcgttcta cccggaatgg ctgacgcgcg gtaaaggtgc ggtgaactac ctgagcgtgc
     8641 cggaattccc gaccgacagc aaaaacggca gcttcctgtt cccgggcggc tacattgaga
     8701 atgcggatct gtcctcgtat cgtccgatca cttctcattc cgatgaatac ctgatcaaag
     8761 gcattcagga aagcgcgaag cactcctggt ataaagacga agcgccacag gcgccgtggg
     8821 aaggtaccac cattccggct tatgatggtt ggtctgacga cgggaaatat tcctgggtga
     8881 aatcaccgac tttctacggc aaaacggtag aagtgggtcc gctggctaat atgctggtga
     8941 aactggcggc aggtcgagaa tctacccaga acaaactgaa tgaaatcgtt gcgatttatc
     9001 agaaactgac tggtaacacg ctggaagtgg cgcaactgca ctctacgctg ggccgtatta
     9061 ttggtcgtac cgttcactgc tgcgaattgc aggatatcct gcaaaaccaa tacagtgcac
     9121 tgatcaccaa tatcggcaaa ggcgatcaca ccaccttcgt gaaaccgaac attccggcaa
     9181 cgggtgagtt caaaggtgtt ggcttcctcg aagcgccgcg cggtatgctc tctcactgga
     9241 tggtgatcaa agacggtatc atcagcaact accaggcagt tgttccatca acctggaact
     9301 ctggtccgcg taacttcaat gatgacgtcg gtccttacga gcagtcgctg gtgggtacac
     9361 cggttgctga tccgaataaa ccgctggaag tggtgcgtac cattcactcc ttcgacccgt
     9421 gcatggcctg tgcggtacac gtagtggatg ctgacggcaa cgaagtggtt tcagtgaagg
     9481 ttctgtaatg cgtattttag tcttaggggt cggcaatatt ttgctgaccg atgaagccat
     9541 cggtgtgcgg attgttgaag cgttagagca acgatacatt ctgccggatt atgttgagat
     9601 cctcgatggc ggcacggcgg gaatggagct gcttggcgac atggcaaatc gcgatcatct
     9661 gattattgcg gatgccattg tgtcgaagaa gagcgcgccg ggaacgatga tgatcctgcg
     9721 ggatgaagaa gttccggcgt tgtttaccaa caaaatctct ccgcatcagc ttggcctggc
     9781 cgacgtcttg tcggccctgc gcttcaccgg cgagtttccg aaaaagctga ccctggtcgg
     9841 cgtgatcccg gaatcgctgg agccacacat cggcttaacg ccgacggttg aagcaatgat
     9901 tgaacctgcg cttgagcagg ttctggctgc gctgcgcgaa tcaggcgtgg aagccatccc
     9961 acgggaggcg attcatgact gaagagatag caggtttcca gacctccccg aaggcgcaag
    10021 tacaggcagc gtttgaagaa attgcccggc gttcgatgca cgatctctct tttctgcatc
    10081 cttcaatgcc ggtgtatgtt tctgatttta cgctgttcga aggtcagtgg acggggtgtg
    10141 tgatcacccc gtggatgctg agtgcagtta tcttccccgg cccggatcaa ctctggccgc
    10201 tgcgcaaagt gagtgaaaaa attggtctgc aactgccgta tggcaccatg acctttaccg
    10261 ttggcgaact ggacggtgtt tcgcaatacc tctcctgttc gctgatgtca ccgctttcgc
    10321 acagtatgtc gattgaagag ggccaacgcc tgacggatga ctgcgcacga atgatccttt
    10381 cgctgccagt cacgaatccg gatgtaccac acgcagggcg tcgcgccctg ctgtttggtc
    10441 gcaggagtgg cgaaaatgca tgagttgtcg ctttgccaga gcgccgttga aattatccaa
    10501 cggcaggcgg agcagcacga tgttaagcgc gtcaccgccg tgtggctgga aattggcgcg
    10561 ctctcctgcg ttgaggagag cgccgtccgt tttagttttg aaattgtctg ccacggaacg
    10621 gtggcgcaag ggtgcgattt acatatcgtc tataaacccg cccaggcctg gtgctgggat
    10681 tgcagccagg tggtggagat tcatcagcac gatgcgcagt gtccgatctg tcatggcgag
    10741 cggttgcgtg tcgataccgg cgattcgctg atcgtcaaaa gtattgaagt tgaataaccg
    10801 gagttaataa tgtgtattgg cgttccaggc caggtgctgg ctgtcggtga agatattcac
    10861 cagcttgcgc aggttgaagt atgtggtatc aagcgcgacg tgaatatcgc cctgatttgt
    10921 gaaggtaacc ctgccgatct gctgggccag tgggtgctgg tacacgtcgg atttgccatg
    10981 agcatcatcg acgaagatga agccaaagcc acattagacg cactgcgcca aatggattac
    11041 gacattacca gcgcgtgatg attagctttc gtaggcctga taagacgcgg cagcgtcgca
    11101 tcaggcattg tgcacgattg ccggatgcgg cgtgaacgcc ttatccggcc tggatggctt
    11161 gctgcgacga acaccaaccc ttacccctga cgcttatctt ccgtattcgt ctcgaaatca
    11221 ctggcgtcat ggcgctcatg caactgctca ttcagcggtc cgttggtgcg gttaacaata
    11281 cgcccacgtt tcaccgccgg acgttcgcct acttctttcg cccagcgttg tacatgctta
    11341 taactgcccg catcaagaaa ctctgcggca tcatacacgc cgcctaacac cacattgcca
    11401 aaccacggcc aaatcgccat atccgcaatg gtgtactcat cgcccgcaat aaacttatgc
    11461 tgcgccagtt gcttatccag cacgtcgagc agacgtttgg cttccatggt aaagcggttg
    11521 atggcgtact caattttcat cggcgcgtaa tggtaaaagt gaccaaaacc accgccgagg
    11581 aacggtgccg cgccctgtaa ccagaacagc cagttcatcg tttcagtatg ctttgccaaa
    11641 tcctgcggca ggaagtagcc aaatttctcc gccagataaa gcaggatcga accagattca
    11701 aacacgcgga tcggcggatt atgcgtatga tcgcgcagcg ccgggatctt cgagtttggg
    11761 ttcacttcga caaagccgct ggagaattga tcgccatcgc caatacgaat cagccaggcg
    11821 tcgtactctg caccagtaac gcccagcgcc agcagctcct caagcataat cgttactttc
    11881 tgaccgttcg gcgttcccag cgaataaagt tgcaatgggt gtttgccaac gggcagcgtt
    11941 ttttcatgcg tcggaccaga aaccgggcga ttgatattgg cgaacgcgcc gccagcggat
    12001 ttatcccacg tccagacttt cgcgggctga taagtattgt ctgtcatagt gagttgcctt
    12061 ctgagtggta gtgttgaagc agtgtagcag gtcacttgct gtacagaatg tgaatttttt
    12121 aaccctttgc gccagccgct gcgatgaggt gtatgattag tctgacctct gtccggttaa
    12181 tgtcgctgag caggtaatca gagcacaaca attacgccaa ctgcttaggt tgtattgtta
    12241 aaggtaaagt gatgagcaaa ggaacgacca gccaggatgc cccgttcggg acattattgg
    12301 gctacgcccc aggtggggta gcaatctact cttcagatta cagttctctc gatccgcagg
    12361 aatacgaaga tgacgccgta ttccgtagct atatcgacga cgaatatatg ggccacaagt
    12421 ggcaatgcgt tgaatttgct cgccgttttc tctttctgaa ttacggtgtg gtctttactg
    12481 acgtgggtat ggcgtgggag attttctcgc tgcgcttcct gcgagaagtg gttaatgaca
    12541 acatcctgcc attgcaggca tttcctaacg gctcgccgcg tgcgccggtc gcgggtgcgc
    12601 ttcttatctg ggataaaggc ggtgaattta aagacactgg ccatgtcgcc atcattaccc
    12661 aattgcatgg caacaaagtc cgtattgcgg aacagaacgt gattcattcc ccgttgccgc
    12721 aagggcaaca gtggacgcgt gagctggaga tggtggtcga aaacggctgc tataccctga
    12781 aagacacttt tgatgacacc accattctgg gctggatgat ccagacggaa gatactgaat
    12841 acagcttacc gcagccggaa attgcaggcg agctgctgaa aatcagcgga gcgcgcctgg
    12901 aaaacaaagg ccagtttgac ggtaaatggc tggatgaaaa agatccgctg caaaacgcct
    12961 atgtgcaggc caacggtcag gtgatcaatc aggatcctta tcattactac accattaccg
    13021 agagtgccga gcaggagcta attaaagcca ccaacgagct gcacctgatg tatcttcacg
    13081 caaccgacaa ggtgctgaaa gatgacaacc tgctggcgct gttcgacatc ccgaaaatcc
    13141 tctggccacg tttgcgtctc tcctggcagc gtcgccgtca ccatatgatc actggtcgta
    13201 tggatttctg catggatgag cgtggcctga aggtttacga gtacaacgcc gactccgcct
    13261 cctgtcatac cgaagcgggc ttgatcctcg aacgttgggc ggagcagggc tataaaggca
    13321 acggcttcaa tccggcggaa gggctgatta acgaactggc tggtgcctgg aaacacagtc
    13381 gtgcacgtcc gtttgtccat atcatgcagg acaaagatat cgaggaaaac tatcacgcgc
    13441 agtttatgga gcaggcgctg caccaggcgg gctttgaaac gcgtatcttg cgcgggctgg
    13501 atgaactggg ctgggatgct gccgggcaac tgattgatgg ggaaggacgg ctggttaact
    13561 gcgtgtggaa aacctgggcg tgggaaaccg cgtttgatca gattcgtgaa gttagcgacc
    13621 gtgagtttgc tgcggtgcca atccgtaccg gtcatccgca aaacgaagtg cgtcttatcg
    13681 acgtattgct gcgcccggaa gtgctggtct ttgagccgct gtggacggtg atccccggca
    13741 acaaagcgat tctgccgatc ctctggtcgc tgttcccgca ccatcgttac ctgctggata
    13801 ccgatttcac tgttaatgat gaattggtga aaacaggtta cgcagtgaaa ccgattgccg
    13861 gtcgctgtgg tagcaatatc gacctggtca gccatcatga agaggtgctg gacaaaacca
    13921 gcggtaaatt tgccgagcag aaaaacatct atcagcaact gtggtgtttg ccgaaagcgg
    13981 acggtaaata cattcaggta tgtaccttca ccgttggcgg caactacggt gggacgtgtt
    14041 tgcgcggtga tgaatcactg gtcatcaaaa aagagagtga tattgaaccg ttaattgtgg
    14101 tgaaaaagta atacatgttg tagcaacccc gtactgatta tgaaga
//
LOCUS       DAPHDX010000113        13961 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPHDX010000113 DAPHDX010000000
VERSION     DAPHDX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.114"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            268..1413
                     /locus_tag="RH058_003418"
     CDS             268..1413
                     /locus_tag="RH058_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV3138965.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1507..2040
                     /locus_tag="RH058_003419"
     CDS             1507..2040
                     /locus_tag="RH058_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV3138966.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(2037..2354)
                     /gene="sugE"
                     /locus_tag="RH058_003420"
     CDS             complement(2037..2354)
                     /gene="sugE"
                     /locus_tag="RH058_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3138967.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3082..8568
                     /locus_tag="RH058_003421"
     CDS             3082..8568
                     /locus_tag="RH058_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV3138968.1"
                     /translation="MINFKPKIPAMLGALAVLTAGAAHAELLEYTFKAPDGTQRSLTP
                     NANYANPTGNISFALSAGIDRKVKISVLRSDGTVVSTATSHLLGATDRITVGGKSYYG
                     AELQLPAPVGGAYTIRAEILASDGSTVQTDEYPLTVDVTPPTYSSLAPVYSNYGQVTS
                     GDVWKLGLGGSEDNAFLLSGISDESPIKGVKAKLYRQDGSLYKDVSVNYDDANGQARQ
                     SFQSGFFPASDLDEVFTLQFEISDSAGNSYLSPRQKVMFDSITNAPSAPFGVYDPSST
                     NNLGPGLTGFVAYTEGMTVKTNPIKLAWRVPRDNWHEYREGGINMTNALGEMSKVGED
                     ASYVYLVTTAPYGNTDGNYWRWVNFGQWGGGGIAYNLTLSPSAPKSPRLLGVDYNYSD
                     IGWSSFYRYWVNNSVLPVTVSSIRVKVEPRPYVQTAVHRGSCEIPVGQDSCVIANSFT
                     MAKGTTGYVHDNATVFNPDKSLRSNPLWAEVNWNDQHYPQLSQQFDQNSKVFTLFVNQ
                     PGRGAYFDRLRLRSAWIEDSKGNKLSPTGGLIANNWENYTYQWDLKTLPEGQYSLVAA
                     AEEMHGPLTRQPMFQITSDRTPPTMTLSVADGAAIQTLDDVVITLADAIDPSPKLTSI
                     ALVGGPANDKVQLSWREESKGRFRLEYPVMFPSLKEGESYTLTVSGEDAQGNAVQKAV
                     GFEYKPRQVMLADGMDGKVMVPAVTHEFVHADGKRIIETKPLTLSDGAVVTGSYDVFA
                     TLRSDAKVPLVVNGVRIEPGQTMGIMSQHDFGASGGRLSIPVKPAVPDVVGSSSLLVM
                     TSAPNSPILVVDINTWKGTAKLSAESWTIRQVIDPVKIYALPESGVPCRFTTKEDVAM
                     AADPIRDPVCLLQWDRTPDEAEQTTQDNNGMKVAGLVGQAVSIGEQPVEYSLYLFSGD
                     GSKVKVGSGSQNLTVTTAYGSVGYTPIDDIAQVNRVIEDFDVNFKQSKGPDCSITLSA
                     DRAKKEAANKAVGSASRACLFEWQQIPDGLVQDPLSESPSLSGSLASNGVHPLGWRVS
                     IFTRNGTRVTLNDETFNVEAVDPPAPTVELASDYNFKDNIYLVPMTGNYLGDAIINSE
                     RADLDIAISRNSDVLESETFTPGWGATNKVYRRINTDERALWEETTYKVNAAYNKVPD
                     VKTEVVYRAISAPSDSIRPIVEVKGDTAIDTQALPVRVLIRDQYKPDGDYDANTMGVW
                     KVRLIQQKAYNETVALTDYAEASNGEAQFSVDLSGVDTSSVRIAAEAVLESPVEGYNR
                     TELSIRPAFLTVLRGGAIGAGVEARKLSGEAPFTAVFKLSLDDRQDLRATGQVVWETS
                     KDDGKTWEQFIPEDRYKYQLVKTFDKGEYQVRAKVVNVNSGAEKYTEAVSVVAYDKPD
                     IAVIGPTTLFVGSEGKYTANLTLNDEPISGGNAIVEWSTDGGKTYAQTGDSITLSSDE
                     ETRYRLWARVRSATAPADDGYAYEVAKTAVDFRAVKAPRPYVTGPRVIETGKKYVFKA
                     ETSLPYRGMDVKLNGFFTLPDGSIVQGDTAEYEPSDTDLNQATVETKYTTWIEGYRDQ
                     GAEASHSLRSRVWQYVWPSFGMQVRKNADVAPATITASVRPIAFNGKLEEPTYEWELP
                     EGAVIQDQRQDIVRSFVINEPGDYNIKVTVRDARGHETVIEQPLKIGQAAPYAIDLQY
                     SGSNKYEREPLDVLLRPYISGGHPRDRISTRVYSVDGTPLESSGYYGRATLGAGEHSI
                     KLKITSEMGHEAEGEVNINVAENKLPACSLSSRETVGSWIVYANCEDTDGRMKSYEWT
                     IAGELQSISSDRVTISKGTYETMPTISLVGVDDSGGKSEAVTMN"
     gene            8717..9424
                     /locus_tag="RH058_003422"
     CDS             8717..9424
                     /locus_tag="RH058_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HDV3138969.1"
                     /translation="MRTKLLGALMVFGIITGTAHASSKLEITDPRAAKIEDIVELPIK
                     GVRAVQSDGQIMFLSENGRFVISGQIYDLWSKKPLNTMSQMRDVAERIHFKSMGMDVD
                     TLNTVSMGRGDKEVVVFVDPRCAVCHQLMGDAKSLVDDYTFKFIVIPALGAESNRLAK
                     NLYCAKDKTHALDALMNNTLGSLPSKETCDPGQYDQTLLTAHFIGIEGVPFVVAPDGR
                     VSKGRPKNLKSWLESAE"
     gene            9421..10122
                     /locus_tag="RH058_003423"
                     /pseudo
     CDS             9421..10122
                     /locus_tag="RH058_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
     gene            10423..10767
                     /locus_tag="RH058_003424"
     CDS             10423..10767
                     /locus_tag="RH058_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HDV3138970.1"
                     /translation="MTKRQRKNRKRINRLVEHWPELFNREKPQPRKVEIPDDLIQDIA
                     IRELAFSAGALRAAVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     VLNDRQKARKPR"
     gene            complement(10786..11334)
                     /locus_tag="RH058_003425"
     CDS             complement(10786..11334)
                     /locus_tag="RH058_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138971.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTACPSSPVVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASATVDEECSQRVDLLQQTWKVISAGGQCQVERCFRVARGDTSGQYVALKTVALSL
                     GLPVVTAITHRPVQRCTLITAQ"
     gene            complement(11603..11839)
                     /locus_tag="RH058_003426"
     CDS             complement(11603..11839)
                     /locus_tag="RH058_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3138972.1"
                     /translation="MLTSITGHDSVLLRAEDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(11908..12480)
                     /locus_tag="RH058_003427"
     CDS             complement(11908..12480)
                     /locus_tag="RH058_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138973.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKPYQKQITRDVVLDRRSPDCEALFKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPWYPVAGQL"
     gene            complement(12738..13304)
                     /locus_tag="RH058_003428"
     CDS             complement(12738..13304)
                     /locus_tag="RH058_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV3138974.1"
                     /translation="MPKSYTPNWFFTALLDNHINQLVARYSRLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWLDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRKPHF"
BASE COUNT         3620 a         3441 c         3600 g         3300 t
ORIGIN      
        1 taagtatcat tgcagcaaag atgaaatcaa tgatttatca aaaatgattg aaaggtggtt
       61 gtaaataatg ttacaatgtg tgagaagcag tctaaattct tcgtgaaata gtgatttttg
      121 aagctaataa aaaacacacg tggaatttag gaaaaactta tatctgctgc taaatttaac
      181 cgtttgtcaa cacggtgcaa atcaaacaca ctgattgcgt ctgacgggcc cggacacctt
      241 tttgctttta attacggaac tgatttcatg atgaaaaaat cgttatgctg cgctctgctg
      301 ctgacagcct ctttctccac atttgctgcc gcaaaaacag aacaacagat tgccgatatc
      361 gttaatcgca ccatcacccc gttgatgcag gagcaggcta ttccgggtat ggccgttgcc
      421 gttatctacc agggaaaacc ctattatttc acctggggta aagccgatat cgccaataac
      481 cacccagtca cgcagcaaac gctgtttgag ctaggatcgg ttagtaagac gtttaacggc
      541 gtgttgggcg gcgatgctat cgcccgcggc gaaattaagc tcagcgatcc ggtcacgaaa
      601 tactggccag aactgacagg caaacagtgg cagggtatcc gcctgctgca cttagccacc
      661 tatacggcag gcggcctacc gctgcagatc cccgatgacg ttagggataa agccgcatta
      721 ctgcattttt atcaaaactg gcagccgcaa tggactccgg gcgctaagcg actttacgct
      781 aactccagca ttggtctgtt tggcgcgctg gcggtgaaac cctcaggaat gagttacgaa
      841 gaggcaatga ccagacgcgt cctgcaacca ttaaaactgg cgcatacctg gattacggtt
      901 ccgcagaacg aacaaaaaga ttatgcctgg ggctatcgcg aagggaagcc cgtacacgtt
      961 tctccgggac aacttgacgc cgaagcctat ggcgtgaaat ccagcgttat tgatatggcc
     1021 cgctgggttc aggccaacat ggatgccagc cacgttcagg agaaaacgct ccagcagggc
     1081 attgcgcttg cgcagtctcg ctactggcgt attggcgata tgtaccaggg attaggctgg
     1141 gagatgctga actggccgct gaaagctgat tcgatcatca acggcagcga cagcaaagtg
     1201 gcattggcag cgcttcccgc cgttgaggta aacccgcccg cccccgcagt gaaagcctca
     1261 tgggtgcata aaacgggctc cactggtgga tttggcagct acgtagcctt cgttccagaa
     1321 aaaaaccttg gcatcgtgat gctggcaaac aaaagctatc ctaaccctgt ccgtgtcgag
     1381 gcggcctggc gcattcttga aaagctgcaa taactgacga tgaggcccag gatattgggc
     1441 ctcctttctt tctctttttt tcctgttgtc atctacactt aacaaaaata cagcaaggaa
     1501 aatcccatgc gcattttgcc cgtcgttgct gcagttacgg ctgcattcct ggttgtcgcg
     1561 tgtagctccc cgacaccgcc gaaaggcgtt accgtggtaa ataactttga tgccaaacgc
     1621 tatctgggaa cctggtatga aattgcgcgc ttcgaccatc gtttcgagcg cggattggat
     1681 aaagtgaccg caacatacag cttgcgcgac gacggcggca tcaacgttat taacaagggc
     1741 tataaccctg acagggagat gtggcagaaa acggaaggga aagcctattt caccggcgac
     1801 ccaagcagag ccgcgcttaa ggtttctttt ttcggcccct tctatggcgg gtataacgta
     1861 attgcactcg accgggaata tcgtcacgcg ctggtttgtg gtccggatcg cgactacctg
     1921 tggatccttt cacggacccc tactatttca gatgaaatga aacagcaaat gttagccatc
     1981 gcgacccggg aagggtttga agtgaataaa ctgatttggg tgaaacagcc tggcgcttag
     2041 tgagtgctca gcttcagacc aataatgcca gcaacgatca gcccaaggct cagcaaacgt
     2101 gccgggctgg cagactcacc cagcagcaaa atccctgtaa tggccgcccc aacagcgcca
     2161 ataccggtcc agaccgcata agcggttcct acaggcaacg tgcgcattgc ccaagagagc
     2221 atggcgatac tgacgatcat cgccgcaata gtgataatgc ttggcgtaag acgcgtaaaa
     2281 ccgtgggtgt atttcaggcc aatcgcccag acaacttcga gcaaacctgc aattaataaa
     2341 acgatccagg acatatcagg ctccagaaca atggggccgt ccccggtgaa agaagcgttt
     2401 gcaggtcgtc ctgcaaagct aatgtgtgaa atggcatttt tgcccggaag aaaatgaatt
     2461 tcaacctttt tattcaccgc ctgctaaaag caagaattaa gcataattag cggcgtagtt
     2521 cccgcatttg gcactcatcg gagttctcta tgatgaaggg ctttctgaaa ccagaaagtc
     2581 gattgcccct cttctgaatg cgaaacgttt atgcttaaaa ttttagtgat tgaccggtgc
     2641 cactttaccc gcacagggat agaggccttg cttaatcatt ctggcaggtt cagctcctca
     2701 tttctggtat caggaatcaa taatctcctg ttagcaaaag agcatatttt gcaatggaaa
     2761 ccgcatctgg tgatcgcgga tttatacagt tttattagcg agacgcactc cagtccgcca
     2821 attaaacctt tttttatgag ttgtggcgtc atcccactaa ttttactgca atcagcagac
     2881 agacagcatg cacccatcgt gccctctcaa tccgtagccc attcggtctt gaccaagcac
     2941 accacgctga acacactctc ccacacaatt caggatttcc ttatttgctc aaaaagggca
     3001 cttccacagc ccatgtttat gcactccctc ccgtaacctc cagcattggg ttcttgtaac
     3061 tcaaaacgaa tggagaatgc gatgatcaat ttcaagccta aaattccggc gatgcttggg
     3121 gctctggcgg ttcttaccgc tggtgctgct catgcagagc tgctggaata cacctttaag
     3181 gctccagatg gtacgcaacg gtctctgact ccgaatgcca actatgccaa cccaacgggc
     3241 aatatctcgt ttgccttgag tgccggtatc gaccgaaagg taaagatttc ggtgcttcgg
     3301 tcggatggaa cagtggtttc gacagcgacc agccaccttc ttggggctac tgatcgcatc
     3361 acagtgggtg gaaaatccta ctatggtgcg gaacttcaac taccagctcc agttggtggg
     3421 gcatacacta tcagggcgga aattcttgcc tctgatggtt ctacagtaca gacggatgaa
     3481 taccctctta ctgtcgatgt gacgccgcca acctactctt cgcttgcccc ggtttatagt
     3541 aactatgggc aggttactag cggtgatgtt tggaaactgg gtctaggtgg ctctgaggat
     3601 aatgcctttt tgctgtcagg aatctctgat gagtcaccca tcaaaggcgt caaagcaaag
     3661 ctgtatcgtc aagacggttc actctacaaa gatgtatccg tgaactacga tgacgctaac
     3721 ggacaagcta gacagtcttt ccaatctggc tttttcccag cctctgactt ggatgaagtg
     3781 tttacacttc agtttgagat aagcgatagc gctggaaata gctatctttc acctcgtcaa
     3841 aaagtgatgt ttgatagcat aaccaatgct ccgtctgcac cttttggtgt ttatgaccct
     3901 tcaagtacaa acaacttagg ccctggatta accggatttg ttgcttacac tgagggcatg
     3961 acagttaaaa ctaacccaat caagttagct tggcgtgttc cgcgtgataa ctggcatgag
     4021 tatagagaag gtgggataaa tatgaccaat gccttgggag agatgtccaa ggtaggagaa
     4081 gatgccagct atgtttatct cgtcacaacc gcaccttatg gcaataccga cggcaattat
     4141 tggcgttggg tgaacttcgg ccagtggggg ggaggcggta ttgcttataa cctcacactc
     4201 tctccatcag cgcctaaaag tcccagactt ttgggtgtgg actacaatta cagtgatata
     4261 ggatggtcta gtttttaccg ctactgggtg aacaattctg ttcttcctgt cactgttagt
     4321 agtatccgtg tcaaagttga accaaggcct tacgttcaaa ctgccgttca caggggaagc
     4381 tgtgaaattc ctgttggtca agatagctgc gtgattgcaa atagcttcac aatggctaaa
     4441 ggcacaacag ggtacgtcca cgataatgcg acagttttca atccggataa atctcttcgc
     4501 tcgaatcctc tttgggcaga ggttaactgg aacgatcagc actaccctca gctaagtcag
     4561 cagttcgatc aaaatagcaa ggtatttacc ttattcgtta accagccagg acgaggcgct
     4621 tactttgata ggctgcgtct gcgaagtgca tggattgaag atagcaaagg caacaagctg
     4681 tctccaactg gtgggttaat agccaacaac tgggagaact acacttacca gtgggatctt
     4741 aaaactctcc ctgaggggca atacagtctt gttgccgcag cagaggagat gcacgggcct
     4801 ttaacgcgac aaccaatgtt ccagataacg tctgatagaa cgccaccaac catgacactc
     4861 agtgtggcag atggagcagc tatccagact ctagatgatg ttgttattac cttggctgat
     4921 gctatcgacc cttcacctaa attgacctct attgcccttg taggggggcc tgccaatgac
     4981 aaggtgcagt tgtcttggcg tgaggagtcg aaaggtcgat tccgccttga gtacccggta
     5041 atgttccctt ctttgaagga aggggaatct tacacgctga ctgtttccgg tgaggatgca
     5101 caaggcaatg cggttcaaaa ggctgttggc ttcgagtaca aacctcgtca ggtaatgctg
     5161 gcggatggga tggatggcaa ggtcatggtc cccgctgtca ctcatgaatt tgttcatgca
     5221 gatggcaaac ggatcatcga aaccaagccg ctgacgctca gtgatggtgc tgtcgtgaca
     5281 ggttcatacg acgtgtttgc gaccctacgt tctgatgcga aagtaccgct ggtggttaat
     5341 ggggtgcgta tcgagccagg ccagacaatg gggatcatga gccaacatga tttcggtgcg
     5401 tcaggtggtc gtttgagcat tccggttaaa cctgctgttc ctgatgtggt cggctcttcc
     5461 agccttcttg tcatgacctc cgcgccgaac tcacccatct tggttgtgga catcaatacc
     5521 tggaaaggga cggctaagct ctctgctgaa tcatggacaa ttcgccaggt cattgaccca
     5581 gtgaaaatct atgccctgcc agagtcgggt gtgccttgcc ggttcaccac gaaagaggat
     5641 gtggctatgg ccgcagaccc aattcgtgac ccggtgtgtt tgctccagtg ggacagaact
     5701 ccagatgagg ctgaacaaac tacgcaggac aacaacggga tgaaagttgc tgggctggtg
     5761 gggcaggctg tgagcattgg tgaacaacct gtcgaataca gcctgtacct gttcagtggt
     5821 gacggttcca aggtaaaagt tgggtctggc tctcagaacc tgacggtaac tactgcctat
     5881 ggctctgttg gctacacccc gattgatgac attgctcagg tgaatcgcgt cattgaagat
     5941 tttgatgtga acttcaagca gagcaagggg ccagactgtt ctataaccct cagcgcagac
     6001 cgtgcgaaaa aagaggccgc gaacaaggct gttggtagtg caagccgtgc ctgtctgttc
     6061 gagtggcagc agatccccga tggattggtc caagacccgt tatcggaatc accttcgctc
     6121 tctggttcat tggcctccaa cggcgttcat ccactggggt ggcgggtaag tattttcacc
     6181 cgtaacggca ctagggtgac gttgaacgac gagactttca atgtcgaagc ggttgaccca
     6241 ccagctccga ccgttgaact agcctccgac tacaacttca aagacaacat ctacttggtg
     6301 ccgatgacag gtaactacct gggtgatgcc attatcaact ctgaacgagc tgatctggat
     6361 attgccatat cgcgcaactc ggatgttctt gagtctgaga ccttcactcc gggatggggt
     6421 gccaccaata aggtgtatcg ccgcatcaat accgatgagc gagcgttgtg ggaggagacc
     6481 acctacaagg tgaacgcggc ctacaacaag gtgcctgatg tgaagactga ggttgtctac
     6541 cgggctatct ctgcaccttc tgacagtatc cgtcctatcg ttgaagttaa aggtgatacc
     6601 gcgattgaca cccaggcatt gccggttagg gttctcatcc gtgatcagta caaacctgat
     6661 ggtgactatg acgctaacac gatgggggtg tggaaagtac gcctgatcca gcaaaaggcc
     6721 tacaacgaga cggttgcgct cactgattat gcggaagcat cgaacggtga agctcagttc
     6781 tcagtagacc tgtctggtgt ggatacttcc tccgtccgta tcgctgctga ggctgttctg
     6841 gaaagccctg ttgagggtta caaccgcaca gagttgtcta tcagacctgc cttcttgaca
     6901 gtgcttcgcg gtggtgccat cggtgctggc gtggaggctc gcaagttgtc tggtgaagct
     6961 ccgttcactg ctgtgttcaa gctatctttg gacgaccgtc aggatctccg ggctaccggc
     7021 caggttgtgt gggaaaccag taaggacgac ggtaaaacct gggagcagtt catcccagaa
     7081 gatcgataca agtatcagct tgtgaagacc ttcgacaagg gggagtacca ggttcgggcc
     7141 aaggtggtga acgtcaactc aggtgcggaa aagtacaccg aagcggtcag tgttgtcgct
     7201 tacgacaaac ctgatattgc tgttatcggc ccgaccacgt tgtttgtcgg aagtgaaggc
     7261 aagtacacag cgaacctgac gttgaacgat gagccaatct ccggtggcaa tgccattgtt
     7321 gagtggtcta ctgacggtgg caaaacctac gcgcagacag gggatagcat cacgctttcg
     7381 agcgatgaag aaacccgata ccgcctgtgg gctcgtgtgc gctctgccac ggcaccggct
     7441 gatgacggct atgcctatga agttgcgaaa acggcggttg acttccgagc agtgaaggca
     7501 cctcgtcctt acgtgacagg gccgcgagtt attgagaccg gtaagaagta tgtgttcaaa
     7561 gccgaaacca gcctgcctta ccgtgggatg gacgtgaagc tgaacgggtt cttcacgctg
     7621 cctgatggct cgattgtgca gggtgatact gctgagtacg aaccctctga cactgacctc
     7681 aatcaggcta ctgtagaaac gaagtacacc acctggatcg aaggataccg agatcagggt
     7741 gcagaggctt cgcatagcct acgctcccgc gtttggcagt atgtatggcc gagcttcgga
     7801 atgcaggtta ggaagaacgc agacgtggct cctgcgacga tcaccgcgtc agtgcggcca
     7861 attgccttca acggcaagct ggaagagccg acctacgagt gggagttgcc ggaaggcgct
     7921 gtgattcagg atcagcggca ggatattgtc cggtcctttg tgatcaatga gccgggtgat
     7981 tacaacatca aggtcactgt ccgtgatgct cgcggccatg agaccgtgat cgagcaaccg
     8041 ctcaagatcg gccaggcagc gccctatgcc attgacctgc aatactctgg ctcgaacaaa
     8101 tacgagcgtg agcctctgga tgtgctgttg cgaccgtaca tttctggcgg ccacccacgc
     8161 gaccgtattt cgactcgtgt ttattcggta gatggtacac cgttggaaag cagtggttac
     8221 tacggcagag caactctggg cgctggtgag cacagcatca agctgaaaat aacctcagaa
     8281 atggggcatg aagctgaggg cgaggtgaac atcaatgttg cagagaacaa gttgcctgca
     8341 tgtagcctga gctcacgaga gaccgttggg tcgtggatcg tctatgcgaa ctgcgaagat
     8401 accgatggcc gcatgaagtc ctacgaatgg accattgccg gtgagttgca gagcatcagc
     8461 tctgatcgag tgactatcag caagggcacc tatgaaacga tgccgaccat ctctctggtt
     8521 ggggtcgatg actctggggg caaatccgaa gctgttacca tgaactaagg ttcattcctc
     8581 tcaaagcccg gttcagaccg ggcttttttt tgtatcgcgc tggaattgtc caaattgggc
     8641 tattacagag gcgagtctat ttccatcccc cggatacact gcccagcatc cagtaatcca
     8701 atcttagggg atagacatgc ggacaaaatt acttggggcg ctgatggtgt tcgggattat
     8761 taccggcacg gctcatgcgt catcgaaatt ggaaatcacc gatcccagag cggcgaagat
     8821 agaggacatt gtagagctac ccatcaaagg ggttcgagcc gtccaaagtg atgggcagat
     8881 catgttcctc tctgaaaacg gacgatttgt tatttcagga caaatctacg acctgtggag
     8941 caagaagccc ctcaacacga tgtcccaaat gagggatgta gcggagcgta tccacttcaa
     9001 gagcatgggc atggatgtgg acacgctgaa cactgtgtcg atggggcgtg gtgacaaaga
     9061 ggtggtggtc tttgttgatc ctaggtgcgc ggtttgccat cagctcatgg gtgatgccaa
     9121 atcgctggtg gatgattaca cctttaaatt tatcgtgatt ccagctctgg gtgctgagtc
     9181 caaccgcctg gcaaaaaact tgtactgcgc gaaagacaaa acccacgcgc ttgatgcgct
     9241 gatgaacaac accctgggtt cccttccttc aaaagaaacc tgcgaccccg gccaatacga
     9301 ccaaacgctg ctgacagctc atttcattgg gattgagggc gttccgttcg ttgttgctcc
     9361 ggatggtcgt gtcagcaaag gacgtccgaa gaacctgaaa tcatggttgg agagtgctga
     9421 atgatcgtaa ccatcaaaaa gaaactcgaa gaaacgttga tcccggagca cttgcgagct
     9481 gccgggatta ttcctgtcct ggcctatgac gaagacgatc atgtcttcct tatggatgat
     9541 cacagtgcag gctttggttt catgtgtgag cccctgtgtg gtgccgatga aaaagttcag
     9601 gagcgaatga acggtttcct gaatcaggag ttcccgtcga agactacgct ccagtttgtc
     9661 ctgttccgct ccccggacat caatcaggag atgtaccgga tgatgggttt gcgtgatggc
     9721 ttccgtcacg agctgctgac atctgttatc aaggaacgga ttaacttcct ccagcaccac
     9781 acgacagatc gcatatttgc caagaccaac aaaggtatct acgacaatgg cttgatccaa
     9841 gacctcaagc tgttcgttac gtgcaaagtc cccatcaaga acaataaccc gactgaaagc
     9901 gaactccagc agctcgcaca gcttcgcacg aaggtcgaat catcgcttca aaccgttggt
     9961 ctgcgtcctc gcacaatgac ggcggtgaac tacatccgga tcatgagcac catcctgaat
    10021 tgggggccag atgcttcatg gcgacatgac tctgtggatt gggagatgga taagcccatc
    10081 tgcgagcaaa tcttcgatta cggcaccgac gtggaagtca gcttacctgc tgattgcttc
    10141 acacccgggc tcctcgcact gcgcgcgggt tattgtttcg ttttcagccc cggggtcgcc
    10201 aaggctctgg atggggtact acttccattg acagcatgag gtaacaatac tgatggtaac
    10261 gctgaccatt aaccgtaaac cacagggcat tttcagtaaa tcaccggcaa cgccgctgca
    10321 ggataaaatg aacactgcac acaacgtgaa ggccagggaa cggataccac agttgaacga
    10381 taaacagagc cctgctctgc tgaaaaaaca ccgtggcggc atatgacaaa acgccagcgt
    10441 aaaaaccgta agcgtattaa tcgtcttgtg gaacactggc ctgagttatt caaccgggaa
    10501 aaaccccaac cgcgtaaagt tgagatacct gacgacctga tacaggatat cgccatcaga
    10561 gaactggcgt ttagcgcagg tgcattacgc gcggcagtag cgtcttatgt gcagtctccg
    10621 cgctattacc gtgcgttaat ggctggcggc gcccgttacg acctgaaggg gcagccgtgc
    10681 ggggaagtga ctccacagga acagaaagag gcagagaccc ggctaatggt gctgaatgac
    10741 cgacaaaaag ccagaaaacc tcgttgacta cacaactgcg ctgattcact gagctgtaat
    10801 caatgtacag cgctgtaccg gacgatgggt aatggcggtc acaaccggta accccagaga
    10861 caatgcgacg gtttttaacg ctacatactg accggatgta tcaccccggg caacccgaaa
    10921 acacctttca acctggcatt gtccgccagc gcttatcacc ttccaggtct gttgcaataa
    10981 atccacccgt tgcgaacatt cttcatcaac cgtggcactg gcccgcggat ggcaaatgct
    11041 cgtctggctg agcagcaaca ggcatcgtat tgttgctcct ccatgttgtt cccgcaacca
    11101 gcgtaatacc accggcgagg atggacaggc agtgtgatta cgctcagtaa tacgttcgtg
    11161 cacctgtcgg taaaaggaag tacagaatgc atgaatctgt tgcccgttga tgtttccttc
    11221 tgccgaatag agcgtgaagt gaaatgccag cagatttggc cagtggtcca ccgcctgctg
    11281 aacaaaacgc cggatgtctc ccggctctga aattaaggct tcggttattt gcacgaaatg
    11341 ttccttttca aaccctgttg aattttgtta agccggtgca gacacacggg aaggtgcgca
    11401 tcaacaccgg tgtaatgcca gttataaaca atgtgaatgg aatgacgaat cagtcaggaa
    11461 agagctacaa cctgctggct gaccggagag ggtcagcagg aaaatgaacg gtaatgtcgc
    11521 cagcaccagg gacagggctg atttccgcaa taccagcgcc ggcattttgc acattgccag
    11581 cgtaccggca tcacaacacg tttcatgctg ctgctcctcg tgattcctca attcgctgct
    11641 gcatccactg ctccacttca cttttgtacc agcggctgct gcggcccagc ttgatgggtt
    11701 tcgggaaatg gccttcactg atcagcttgt aaaaccattt atcggtcatg ccggtgaaac
    11761 tggtgatgta gttcatgtcg atcaggggat cctcggcacg cagcaataca ctgtcgtggc
    11821 ctgttattga ggtcaacata gtcagaatat cctctgtgag atttatgaag gttgggcaaa
    11881 tgcgggtgaa tctgctgaac tgagctttca gagctggcct gctacgggat accagggatt
    11941 gccggcaagg caacgggctt ccggatcgca cactccacgg cgatcgcggg cgaaatcact
    12001 gaacgcccag ataaggtcct taaacagggc ttcgcagtcc ggtgaccggc gatccagtac
    12061 cacatcccgg gtgatctgct tctggtacgg ctttgcttca cctggtgacg cgaaacccag
    12121 cacgttactc caggcatcca gaatcaaccg atccagttcg gggccgtcga aatactcctg
    12181 tctgacgcat aacaccacgt ggtattccgg cttttcagga tgacgggtca ggagccagca
    12241 aaggtcacgt ccgggtgttg cattcatcag cgcactgacg gtttcgataa aatccatacc
    12301 aaaccaggtc gttctgctgt tgaaggtatt gtcatattca cacggcagac gaagcaggat
    12361 ccgccgtatc cgtgagccgg actgctcacg cagcgcacgc tcaaggacgc cgatgacttt
    12421 gttctgataa gatggccaga agggggccag gctatagtcg aaataaggga tagcattcat
    12481 aagatatgct ccgttatgcg cagcccacaa tgcgtcctgc ccgtgaataa gctgacgtgt
    12541 tatggtttgc ggatggggtt gatggaataa gtgaagaacc ggggcagaac gggaagatca
    12601 ggtcaccgac aagggacaga acaccggatg tggttcagag tgaatgatct ccgttgactt
    12661 cagttcacat cattaagcaa ggcggtaagg ttttaccgac ggaatgtttc caggacagga
    12721 agctcaggat ttaagcttca gaagtgaggc ttacgaggac gccctgcagc aggtcgttca
    12781 ggaacttcac tgcaaccgta tgcgcacagt ccgtcttttt gctcttcttt cgccagataa
    12841 tgcagggcac cgcgaatatt atcgatgctt tcaggatcat tgtggcgcac aggaatgttg
    12901 atgttgtatg tataatgcgg ctgatatgta cagcggtgtg ccgaaccgct gttatgcgta
    12961 atagaccgcc agcattccgt aatccgctcc gcaaagggat atattttttt aaccctctga
    13021 cgatcgagcc agaaaaccgc atgcgcatga aaaccatgat cagccgtcca ttcaatcacc
    13081 cagaagaaac caacgatatt ttcaaattgt tccacctgct ccagcagcat acgcaactgc
    13141 aattcaagcc agcgatgatc aggttgtaag aaatcaggcg tatctttcct gtagaagaaa
    13201 tccatgcgta aggcccgcag gcgggaatag cgtgcgacca actgattgat gtgattgtca
    13261 agtaaagcgg taaaaaacca gtttggtgtg taagatttag gcatgctgat gactcctgaa
    13321 agttaatgac gttttaagtg ctgtaaacga attaatgctt tcgctcataa tcagatctaa
    13381 aacggtaata acttacagac ggtatttatc aggcatgctg taacagaatg gatacgtcgg
    13441 ttattaacga cacgtagcgt tgtgcagaaa taaatggatt atctgatggt attagctaaa
    13501 gagatgagat aaacgaggag cgaataaaat acagaatcaa taaacatcac aaaatactgc
    13561 aaaacacgcg ataaaaaccc acgaccagaa gttcaagaaa catattacga ccaataacat
    13621 cataaactac aaacttgctc tcagtggttc taaaaaaaac gtatacaagg ccaacacaat
    13681 aaacttatac gccctcatcc aatctgttct taaggacatg aacgttaacc gcacacatta
    13741 ccagagtaaa aaataccact ggcatgattt ttaccctgca tcttaaacaa tacaacccga
    13801 aaaaaacata atcaggaatt cactctgtta atttcattgt ttatcctgca ctgttaaaca
    13861 tgacgaaatc atggacacct tatcaaagag taaacattca gttaatcagt gatgacagaa
    13921 gtattgatat ttaccagaat ggttaaaaca tatcagataa a
//
LOCUS       DAPHDX010000114        13905 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPHDX010000114 DAPHDX010000000
VERSION     DAPHDX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.115"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..867)
                     /gene="yhaJ"
                     /locus_tag="RH058_003429"
     CDS             complement(<1..867)
                     /gene="yhaJ"
                     /locus_tag="RH058_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV3138975.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLR"
     gene            972..1673
                     /locus_tag="RH058_003430"
     CDS             972..1673
                     /locus_tag="RH058_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV3138976.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1696..1860
                     /locus_tag="RH058_003431"
     CDS             1696..1860
                     /locus_tag="RH058_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3138977.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2072..3382)
                     /locus_tag="RH058_003432"
     CDS             complement(2072..3382)
                     /locus_tag="RH058_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV3138978.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3410..4741)
                     /locus_tag="RH058_003433"
     CDS             complement(3410..4741)
                     /locus_tag="RH058_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3138979.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5016..6380)
                     /gene="tdcG"
                     /locus_tag="RH058_003434"
     CDS             complement(5016..6380)
                     /gene="tdcG"
                     /locus_tag="RH058_003434"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV3138980.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6452..6841)
                     /locus_tag="RH058_003435"
     CDS             complement(6452..6841)
                     /locus_tag="RH058_003435"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV3138981.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6855..9149)
                     /gene="tdcE"
                     /locus_tag="RH058_003436"
     CDS             complement(6855..9149)
                     /gene="tdcE"
                     /locus_tag="RH058_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV3138982.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9183..10391)
                     /gene="tdcD"
                     /locus_tag="RH058_003437"
     CDS             complement(9183..10391)
                     /gene="tdcD"
                     /locus_tag="RH058_003437"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV3138983.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10417..11748)
                     /gene="tdcC"
                     /locus_tag="RH058_003438"
     CDS             complement(10417..11748)
                     /gene="tdcC"
                     /locus_tag="RH058_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV3138984.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11770..12759)
                     /gene="tdcB"
                     /locus_tag="RH058_003439"
     CDS             complement(11770..12759)
                     /gene="tdcB"
                     /locus_tag="RH058_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV3138985.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12858..13796)
                     /gene="tdcA"
                     /locus_tag="RH058_003440"
     CDS             complement(12858..13796)
                     /gene="tdcA"
                     /locus_tag="RH058_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV3138986.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3604 a         3771 c         3264 g         3266 t
ORIGIN      
        1 ccgcagacac caggatttcg cttcccccat actgtcacga cgccaggcca taataatatc
       61 gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa tatctttttc
      121 gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaatgcct gacgtttatc
      181 ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc agctgtacgg tcaacaccgg
      241 gcgctcacga gcggtatccg ctaccgcaat tccacgatat ttcacgcgcg tcacttcaga
      301 taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa
      361 cgtatagagc ttgcgcgagt tgatctccga cgaggaacga aaatgcatat ccggcgcgat
      421 aacaatatcc gcccgcccct gctccagtcg ttcccacgcc cccgccagca cttcggtgat
      481 gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga aaaaggcagg
      541 tgtcggtacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc gcgcgagggc
      601 ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc gctccagcaa
      661 catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca gcaccacatc
      721 cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc gtcccagctc
      781 atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc gtagtgcttc
      841 cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg aacataccgg
      901 gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga agtaaagaga
      961 ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca gactacggat
     1021 ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa ttgttaggct
     1081 atgcctccct gcgtgtgctt aaccaggaag tgctggcccc aggtgccgcc tttcagccgc
     1141 gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca gagtatcgcg
     1201 acagcgaagg caatcatgtt caggccagcg ccggtgaggc gttgctgctc tctacccagc
     1261 cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa accgttaaca cgaatgcagc
     1321 tttggctgga cgcctgcccg cagcgagaga atccgctgat tcaaaaactg gcgcttaata
     1381 tgggcaagca gcaattaatc gcctcgccag agggcacagt gggtagcctg caactgcgcc
     1441 agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc
     1501 atgggccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt acgcatcatg
     1561 aagagaaagc ggcgctaacc tgcggtgatg gggcgtttat tcgtgacgag gctaacatta
     1621 cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt
     1681 tacagggaga tgatgatgag taaaaaattg gccaaaaagc gccagccggt gaagcccgtg
     1741 gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat gttgagcgag
     1801 ctggaagcta tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc taccgcgtaa
     1861 agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc tacaaaatcc aacaacatta
     1921 acgaattaaa caacccgtag gccggataag atgcgccagc atcgcatccg gcatcaggta
     1981 caaaacacaa caacatcaac aagttgagca ccccttaggc cggataagat gcgccagcat
     2041 cgcatccggc acgataccca aaacctggcg tttatctggc cttgctcgcc ataatctcga
     2101 taatctgccg atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg
     2161 actgctcaac atcatgcgcc acgatccctt cattgccggt cacggcggta tcatccagcg
     2221 ccattaacac cgctttccac gcagccgaag cactggtcga aaccttcatc gcacagctgt
     2281 tcgacgctcc atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg
     2341 agatagtttc ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg
     2401 cggtcgttgc ggcacaaagt gcagacaaac gcggtaactg gttatggatg taaattgcgc
     2461 tcaaatgcga aagcatcagc gcacgcgcca gccgttcatc atccgctccg aagtgttctg
     2521 ctaccaccac cacaggcatt gttgcggtga tcccctggtt acccgagcca gagttactca
     2581 ttgccggaag cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa
     2641 tggatgaaga gagatctttc gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc
     2701 caatatgcag cccccactta ccgctcaaac cttcctgcga taacgcacaa tttagcttcg
     2761 cggaatcgag aataaagcgg atcgccgcaa acgggacttc attgacgaac ttcaggatct
     2821 cagccagcgt cgttctggaa agcaccgtca gcggagactc ttgctcgccc tctgccacac
     2881 acgcctgctg ggtaaacacc acaccattgt gcgtctcaat atgcacaatg ttggtatgcc
     2941 cgccgacgat ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga
     3001 ggatttcatt gcaaggttcc tggatcttaa cggagacttt ccccgccgcc agcagtgctt
     3061 tggcatcggc aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttggcat
     3121 ttccacctaa cgcccccagc gccgccgcaa tcggcagccc caccattccc gtgccgggaa
     3181 cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa
     3241 ccggaccttc cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag
     3301 tacatcccag cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg
     3361 gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg
     3421 ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc
     3481 ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta
     3541 ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga
     3601 tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt
     3661 taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt
     3721 cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata ttgagaatga
     3781 cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca
     3841 gcgcggaaat attctgctca taggctttta ccgcttcgtc atgtcccatc gccagcgtga
     3901 acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata ttcattgccc
     3961 gcaatgcttt atgccgcgcc acttcaattg atttttcccg cgagcgataa gagatcacca
     4021 tcgggcttaa cgtctggata aacagaatcg acgtcagggt aaacggcagc gtaataatgg
     4081 cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga tgccacattc
     4141 ccaccatcga cacgcccagc gccgcgacca ccagcagctt ggtcagcacc atgccggtcg
     4201 aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg caaatcagta
     4261 ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag gtatgcagat
     4321 aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc accagcatta
     4381 cgaaatagag cgcacctaac aggatgcccc agtttttacc taaataaccg ctaatgacac
     4441 tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac cgctgaaaca
     4501 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
     4561 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
     4621 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
     4681 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
     4741 taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga
     4801 tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc
     4861 agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta
     4921 aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaggtgcac
     4981 atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc
     5041 agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc
     5101 gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc
     5161 gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc
     5221 tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca
     5281 ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc cgccattgaa
     5341 caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt
     5401 ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga ccgctcgttt
     5461 accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg
     5521 ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg
     5581 tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc gctggaaacc
     5641 agctgacgac gcagcgctac ggcacgacgc ggcacattga gcggaccagg cagcacgcct
     5701 tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaccct
     5761 gcgtcaattt ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag gccagatata
     5821 gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt
     5881 acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg
     5941 ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc agtgatccgc
     6001 attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga
     6061 aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct
     6121 ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg
     6181 ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc atacagatcg
     6241 accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt
     6301 tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa
     6361 atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga
     6421 acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa
     6481 tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg gtcgggtagg
     6541 tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca
     6601 gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca
     6661 cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct
     6721 caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa
     6781 cgccctgaac ataagggccg attgcgcctg gggcacgttg cgtttcgata atctttttca
     6841 tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc ctgttgctgt
     6901 tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga
     6961 tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg
     7021 aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca
     7081 aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat tgagaaggtg
     7141 tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc
     7201 acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg
     7261 gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata
     7321 gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt aattttcttc
     7381 ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac
     7441 tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt
     7501 ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg
     7561 ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccattagcga agcttcgtag
     7621 ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg caccgccagc
     7681 cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac gtcgtccatc
     7741 agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt
     7801 gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca
     7861 atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc
     7921 atcagatcgt cattttcata ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat
     7981 tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgcc
     8041 ggccccatag tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac cagcgtacga
     8101 ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc
     8161 gaatcaaatt ccggtgtacg caggaagcgt accatacgga tcttcatgat gaagtgatcg
     8221 atcagttcct gtgcctgctg ctcattgagt acgccagctt taaagtcgcg ctcaatgtag
     8281 atatcgagga acgatgccgt gcggcccagc gacatcgcgc cgccattttg cgatttcact
     8341 gccgccagat aagcgaagta gagccactgc accgcttcct gcgcattctg cgccgggcga
     8401 gagatatcaa agccatattt cgccgccatt tcctgaatct gcaacagcgc atgacgatgc
     8461 tctgccagct cctcacgcag acggatggtg gcttccagat cctcgccttt ttccagacga
     8521 gactggagat cggcaaattg cagttcgcgt tcacgtacca gataactgat gccatacagc
     8581 gctacgcggc gatagtcacc gataatgcgc ccacggccat agccatctgg taaaccggtc
     8641 agcacgccag atttacggca gcgcagcata tccggtgagt aaacatcaaa tacgccctgg
     8701 ttatgggttt tacgcagatc ggtaaacaga tattcaaatt cactgtccat ttcacggcca
     8761 taggcgtgga atgaactttt aatcatatta atgccaccga acgggtgtag cgcacgtttc
     8821 aacggcgcat ccgtttgcag gccaacaatt ttttccagcg gctggttaat atatcccgca
     8881 tcatgagcgg taattgtggt ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg
     8941 ttttcgatac ggataccttc cattactttt tcccacaatt ccgtggtggc aggcgtcgct
     9001 tcggcgagga aagattcatc gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg
     9061 acattaattt cgtttttcca gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc
     9121 ttatcgctgg tatcaatatc taccttcatg aaaaataatc tctctacaat acttcaacta
     9181 aattatgcaa attctgcggg cgcgttaact ttgcctaaat gaatggcatc caaagcaatc
     9241 attttttctt cgttagtcgg aataacggca caaatgacac gcgcattttc actggaaaca
     9301 attcgctcac cacaggagtt agagcgatta ttcatttctg tatcaatctc tacgcctaat
     9361 acagccaaat gttccatgac cagacgacga attaagcttg aattctctcc tattccgccg
     9421 gtgaatataa ttccatccag gcgacgtaat gaagctgcgt gtccggcaat atgacgggca
     9481 attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct
     9541 ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc cgactcttta
     9601 ttcactacgc gttccaggtc acccaggctc tggttggttt ggctggcgac ccaggacatc
     9661 gcaccaaagt cgacatcgcc actgcgggta cccatcatca agccttccag cggcgtcatt
     9721 cccattgaag tatcaacact ctgaccgttg cgaaccgcgc agattgacgc gccattgcca
     9781 agatgcgcca caaccaggcc ggagtcatct tccgccagat tcagcagcga atgtgcgcgc
     9841 tgggaaacat agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc taactcttca
     9901 taatatttcc acggcaggcc gtataaataa gcttccggag ccatcgtctg gtggaaactg
     9961 gtatcaaata ccgccacctg agttacgccc ggaaataatt gctgcgccga ttcaatacca
    10021 cttaaattgg cgtaattatg caggggtgcc agtggagaaa cgcgacggat attatcaatg
    10081 acttcatcgg taataatggc ggactcggta aaaatactgc cgccgtgagc gatgcggtgg
    10141 ccaattaagg ccacactgtc atttaaattc cgtttttcca gttcaaatgc aattgccttc
    10201 aatgcacctt cgtagctgtg gtgagccagc ggtgctggct ctcccccatt tacggataag
    10261 aatgcatttt ccgagttaat accgtcggca atacctgaca ttaatacttc acagtcgctg
    10321 gcatcgagta cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga
    10381 aattcattca tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt
    10441 caggatggtc agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt
    10501 cgccagagac ggcgctttac ggatggcata catcggcaac aggcacagca gggatgcgat
    10561 aatcggtgcg cccatggctt caataaggtc gaggatgttc gggttggcgt aggcaacaac
    10621 ccaggtggag cccatgatga agatcatgct gatagtgttc agtttaccca gcgacacttt
    10681 agttttgtcg cctttataac cgaacttcag aatcagacca ttcaagcctt ccagcgtccc
    10741 cagatagtga ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata
    10801 ttccagtgta atcgcgaacg ttgttttggt accggtcatg gacgcaaagt ggttagccag
    10861 ataagaaagc actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagcgt
    10921 aaacaggcag ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga
    10981 aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc
    11041 acgtttagaa accacgaagg aagagacgat tggcgagaag ttaaaggaga aaaccatgat
    11101 ggaaatcccc agccagacag tgatcaggat accgtcatga ccggttaacg acagcgaacc
    11161 gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag
    11221 caccaggctg gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt
    11281 accaaaccag atgacgaaag ccatcagcag caacaggaac agcgccacaa agccgcgatt
    11341 cagcggtgca aatccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac
    11401 gccataaatc cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc
    11461 gccagtttta ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag ggttagagcc
    11521 agaaagacac agacgcgcca gcgcccggtg gcaataaaat gcgatggggt atgccaatac
    11581 caacatcaga agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac
    11641 cccggcgccg attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca
    11701 ggacgattgt tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca
    11761 acgaattaat taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt
    11821 gccgccggaa ataatactga cggtttttct gttttgaata tattggtcta atttaccgct
    11881 taataatgca gcacatgcca gagcgcctgc gccttcggtg acgactttat tgcgctgaat
    11941 taaggcaatc atactgtttc tgatttcgtc ttcgctgacc agcacgatgt catcgactaa
    12001 ttcacgaacg atttcgtaag ttaaattacc cgggcgggag acatcacaac catccgccag
    12061 ggtgccggta gttcggtgcg tggttatttc tccggagtgg aaagaagccg ccatgccgtg
    12121 aacgttttct gactgtacgc caataacacg aatggtcggg ttaatagatt taattgccac
    12181 cgcaatacca gcaattaaac caccaccacc aattggcaca atcacgttat cgacatcata
    12241 gagatcttcc ataatttcca gaccaatcgt tccctggcca gcaatcactt tcggatcatc
    12301 gtaaggtggg ataaaaatac gaccttccat ttcgacaatt tcgctcactt tagcgatagt
    12361 gtcgttgaaa ttatcaccat gcagaacgac ttctgcggag tagtcgcacg ttgccgctac
    12421 tttggatttt ggcgcacctt ttggcatcac cactttaccg tcgataccca gcatcgcgca
    12481 ggagagcgaa accccttgcg catggttgcc cgcagaacag gccactacac ctttacgttt
    12541 ttccgcatca gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa atgaacccgt
    12601 acgctgcata ttttcaaact tcaggaatat ttcacctttg caacgttcac taaaatagtt
    12661 tgagcgaggc atgcctgttt tataaattcg cccagccagt cgttgtttcg cttcaataat
    12721 gtcatcaata gcaaccggca gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa
    12781 ttaaatatgt aggtaaccgt aaccgacacc tgcaaaacag acaggtggat tatttatgtt
    12841 taaataaaaa caaataacta accaacttct attaattgcc ttcgtctaca cccattataa
    12901 gatgaatact ctttggctaa ttccaccaaa accgatgctg cttttttaat acgataattt
    12961 ttcgaccata ccgcggcata ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata
    13021 aattgatttg aaccaaaagg tgacgtcata tcacaaggaa ttacagttaa gaaatcagca
    13081 ttgagaacaa gattataaat tgtcacgact gagtcggttt taacgatgtt ttcaatactg
    13141 atgccatttc tttgtaacgt agtaagaagc tcgctgtagt accccatatt ggtttgtggc
    13201 aacacccact gttcgttctt caacgactcc agcgtggtgg tgccggtgca tgttcgggac
    13261 ttactggcta ccagcacaaa ctcggactcg aacagcggct caacatgtaa atcctgaagc
    13321 ttcatttctg cacttaacgt accaatcgca aaatccagtc gaccgtcgcg gattgccggt
    13381 aagaacgaag acagttgcgc ttcatacata gaaacctgcg ctttcgggaa cacctctttg
    13441 aacttgttga tcatccctga cataaaagta aaaccaatca atgaaggaaa accaaatgag
    13501 acttccacca ccgcctcaga agacataccg cttatctcat taaccatatt tttcatttca
    13561 cgggtaatgg attcggaacg ggagagtaac aattgaccgg caggtgttaa tgttacaccg
    13621 gtatttttcc gcaccactaa ttccacacca aaataatctt caatatcgtt aatgatttta
    13681 ctgacggccg gttgagttaa ccctaattct tttgcagccg agccgataga gccacttcta
    13741 atgacttcct gaaagactac caggtgctgc gttttcggaa gaagaatagt gctcataacg
    13801 acctacgtta attacctcat tgacggcatg aagtgtatca aaatgaaatg aacaggatat
    13861 gtgcgaccac tcacaaatta actttcaata ctttccatgg tatcg
//
LOCUS       DAPHDX010000115        13902 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPHDX010000115 DAPHDX010000000
VERSION     DAPHDX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.116"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(156..902)
                     /gene="cutC"
                     /locus_tag="RH058_003441"
     CDS             complement(156..902)
                     /gene="cutC"
                     /locus_tag="RH058_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV3138987.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDSLQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(916..1482)
                     /locus_tag="RH058_003442"
     CDS             complement(916..1482)
                     /locus_tag="RH058_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3138988.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1698..3431
                     /gene="argS"
                     /locus_tag="RH058_003443"
     CDS             1698..3431
                     /gene="argS"
                     /locus_tag="RH058_003443"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV3138989.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3608..4096
                     /locus_tag="RH058_003444"
     CDS             3608..4096
                     /locus_tag="RH058_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV3138990.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4216..4608)
                     /gene="flhe"
                     /locus_tag="RH058_003445"
     CDS             complement(4216..4608)
                     /gene="flhe"
                     /locus_tag="RH058_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV3138991.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4608..6686)
                     /gene="flhA"
                     /locus_tag="RH058_003446"
     CDS             complement(4608..6686)
                     /gene="flhA"
                     /locus_tag="RH058_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV3138992.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6679..7827)
                     /gene="flhB"
                     /locus_tag="RH058_003447"
     CDS             complement(6679..7827)
                     /gene="flhB"
                     /locus_tag="RH058_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV3138993.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8029..8673)
                     /gene="cheZ"
                     /locus_tag="RH058_003448"
     CDS             complement(8029..8673)
                     /gene="cheZ"
                     /locus_tag="RH058_003448"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV3138994.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8684..9073)
                     /gene="cheY"
                     /locus_tag="RH058_003449"
     CDS             complement(8684..9073)
                     /gene="cheY"
                     /locus_tag="RH058_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV3138995.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9088..10137)
                     /gene="cheB"
                     /locus_tag="RH058_003450"
     CDS             complement(9088..10137)
                     /gene="cheB"
                     /locus_tag="RH058_003450"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV3138996.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10140..11000)
                     /gene="cheR"
                     /locus_tag="RH058_003451"
     CDS             complement(10140..11000)
                     /gene="cheR"
                     /locus_tag="RH058_003451"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV3138997.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11019..12620)
                     /gene="tap"
                     /locus_tag="RH058_003452"
     CDS             complement(11019..12620)
                     /gene="tap"
                     /locus_tag="RH058_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV3138998.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQMEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12666..>13902)
                     /locus_tag="RH058_003453"
     CDS             complement(12666..>13902)
                     /locus_tag="RH058_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDV3138999.1"
                     /translation="YGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADD
                     YRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGR
                     SEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEE
                     TAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIA
                     DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKAL
                     IEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAVS
                     EMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQ
                     PPAQPRLRIAEQDPNWETF"
BASE COUNT         3329 a         3895 c         3415 g         3263 t
ORIGIN      
        1 ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc gttcgttttt
       61 tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
      121 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
      181 attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata ctcgtccgcg
      241 tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga ggcttgccac
      301 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct
      361 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
      421 atttttgata aaccttgcag tgagtctgat ttttgccctg atgtcagtac ccgggcaatg
      481 cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc gaaggcgcga
      541 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
      601 acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa ttcgcggacc
      661 gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
      721 cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtacc
      781 cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg
      841 ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc
      901 atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa tggaccacgg
      961 atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc
     1021 atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga gtccttcatc
     1081 agaaagcaaa gccagcgcac gggcattcag cgtttctgga tcacccggca gaacgatttc
     1141 aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta gctccacaat
     1201 agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct
     1261 gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc gacgccagcg
     1321 ctcagcggtg acgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     1381 attcaacccc agacggcggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     1441 tgcaatatcc tgcagctcat cgatagattg ccagttcgcc ataatcactc ttcgtctttc
     1501 agtaaaagcg ttaatttacc ctgttgccct gtgtcaacca accgctgatt tcacgccgct
     1561 tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga aaacagccat
     1621 ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat
     1681 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     1741 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc
     1801 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac
     1861 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     1921 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     1981 atgttcagca ggcgctggcg tccgatcgtc tcggtgttgc tacgccagaa aaacagacca
     2041 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcacgtcggt cacctgcgct
     2101 ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc
     2161 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     2221 agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg
     2281 atgcgaaaaa gcattacgac gaagatgaag agtttgccga gcgtgcacgt aactacgtgg
     2341 taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca
     2401 ccatgacgca gaaccagatc acctacgatc gtctcaacgt gacgctgacc cgtgatgacg
     2461 tgatgggcga aagcctctac aacccgatgc tgccaggaat tgtggcggat ctcaaagcca
     2521 aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt ccttgatgag tttaaaaaca
     2581 aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat ctctacacca
     2641 ccactgatat cgcctgtgcg aaatatcgtt atgaaacact gcatgccgat cgcgtgctat
     2701 attacatcga ctcccgtcag catcaacacc tgatgcaggc atgggcgatc gtccgtaaag
     2761 caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctgggta
     2821 aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc gatctgctgg
     2881 atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat atgccagctg
     2941 acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct
     3001 ccaaaaaccg caccacagac tacatcttcg actgggacaa catgctggcg tttgagggta
     3061 ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc cgtaaggcag
     3121 aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc
     3181 aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca
     3241 cgccgcatgt gatgtgtgct tacctgtacg atctggctgg tctgttctct ggcttctacg
     3301 agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg
     3361 cacaactgac agcgaagacg ctaaagctgg gtctggatac gctgggtatt gagacagtag
     3421 agcgtatgta atcgattttt cgtgagagta aagcctgatc agggttacct gatcaggctt
     3481 ttttattgcc atctaaatgt attctgaaaa tggacatgcc attgttttct gactgttgga
     3541 taagaggcca gaagcgtaat atccggcccc agggaaacga taacggttga atttaaggaa
     3601 taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc aggcttacgc
     3661 agaagatacg gttatagtaa acgaccatga catttcagcc atcaaagatt gttggcaaaa
     3721 aaattcagat gatgatactg acattaacgt gatcaaatca tgcctgcgac aagaatacaa
     3781 tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata tagaacaagt
     3841 gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta aaaaatcaca
     3901 gcgagcctgg ctggatttta gggacaaaga atgtgaatta atcctttcaa atgaggacgt
     3961 tcaggattta agtgaccctt attctgaatc agaatggctc tcatgtatga tcatacagac
     4021 caatacgcga actcgccagt tgcagctata ccataactct gaagattttt atccaagccc
     4081 tttgacaaga ggataattca catctttttg gcatgttttg ttgcaagcta ttcctgataa
     4141 ataattgcaa caagacatcg agcctttttc actgagttat taaacatact cgcgagcgcg
     4201 taattttttt gtccttcagc gataattcac aatcacttca ttacgttgta cctttagcgg
     4261 tggaattaac cgcccaccgc ctggcacttc ccagataaat cgcaacggtt ctgctgccgc
     4321 tataccggaa aaggccacgg tggttccgct ctgcccctct aattcgacac aacgagattg
     4381 cgaacacaag cgaacccgca gtcctgaagg tgtcgggccg ataagctgat aacgccacgc
     4441 taccagcgtc atcaatcctg aagcaggttg tcgcgtagaa agaggcgcag acgacatcga
     4501 ctcaccgcga tgatttagcg taattcctac actacttgcc tgccacatcc cctccccggc
     4561 ggcttgcacc agcagcggaa acaataatat tgctaataag gttctcatta tttgccgcca
     4621 attgtcgccg tcatgcggat atgtcggtta tcagacagtt ccagattcga caggaccact
     4681 aactgcggca agctgcggcg caggaagcga gacaataatg gtcgcagcgc gtggttcacc
     4741 aacaatactg gcggcgcacc cagcatctcc tgacgggata gcgcttcctg agtttgcgcc
     4801 agtaaacgat ccgccagccc tggctccagt cctcccccgc cctgcagcgc ctgtagtaac
     4861 aaacgttcca gcggtgtatc gaggccaata acatggactt catctttgcc aggaaaccac
     4921 tgctgggtaa tcgcccgtcc caacgccacg cgcacgacgg cggttaattc atgtggatcg
     4981 ctttggatgg gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggt
     5041 actttttcat cgaggagatt ttgcagcact ttatgcagtg tggtgagcgt gacgacgcca
     5101 ggaacgagat cttccgtcag ctttggcatc tcctgggcga cgcgatccaa cagctgttgc
     5161 gcctcctgac gaccaaacag ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt
     5221 gctaccaccg tgctggcctc aaccactgtg tacccctgaa tctgcgcctg ttcttttagc
     5281 gcactttcaa tccagatagc attcaggcca aatgccggat cgacggtcgc ctcaccaggt
     5341 aacgtcccgg cagcggttcc agggttaatc gccagccagc gccccggata agcatcacca
     5401 ctgccaatct ccacgccttt catcaaaatg cgatagcggg caggttgcag atccatattg
     5461 tcgcgaatgt gcaccactgg cggcagaaat cccatctcct gggcaaattt cttgcggata
     5521 ctgcgtatac ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga
     5581 taacccactt ccattcccag agaatcttcc agttgtacat cgttccacgt cgcttcgaca
     5641 acggtattat tctctgccat ttttaccggt ttgggttcgg caggcgcttt ttgttcgcgt
     5701 ccgcgtatcc accaggccag ccccagcaat ccggcagtga acagcaaaaa taccaggttc
     5761 ggcattccag gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa cataacgctt
     5821 gggttactga aaagctgatt caccatctgc tcgccaacat cctgatcggt gctgacacgc
     5881 gtaacgatga cccccgcggc ggtagaaatc accagcgctg gaatttgtgc caccagaccg
     5941 tcgccaatgg tcaatagcgt ataactttcc gccgcgtgtc ccatgctcat gccatgttgc
     6001 agcacgccga ccagcaaccc gccgacaacg ttaatgacca tgatgaggat cccggcgatg
     6061 gcatcgccgc gaacaaactt acttgccccg tccattgagc cgtaaaaatc ggcttcctga
     6121 gtcacttcgg agcggcgttt tttcgcctca tcttcaccaa tcaatccagc gttaaggtcg
     6181 gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc acccacttct
     6241 gcgatacgcc cggcaccttt ggtaatgacc ataaagttga tgatcacgag aatgacaaac
     6301 accacgatac cgatagcgaa attgccacca acgaggaagt gaccgaatgc ttcgaccacc
     6361 ttcccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt tgaagccacg
     6421 ttaagtgcca gacgcaacaa cgtagtaaac aacagaatgg tcggaaacgc agcaaactca
     6481 agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag
     6541 gtaaacaaca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac
     6601 aggatcaaaa tcggtccggc aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg
     6661 cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggcttc
     6721 cggcaccgga agatgagtag gttgtacagg gcgctgtcca ccagccagac gccagcgttt
     6781 cagttgccag acccaggcca gcacttccgc caccgcggcg tacagttgac ccgggatttg
     6841 ttgaccaatc tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg
     6901 gacgttattt tcagcgccaa tttcacgaat gcgcagcgcg accagccctg cacctttagc
     6961 gaccactttc ggtgcgctca ttttgttttc gtcatactgc aacgctaccg aatagtgggt
     7021 cgggttattg acaatgacat ccgctttcgg cacatcggcc atcatccgac gccgtgcagc
     7081 agctcgctgc atctgacgga tccgcccttt aacatgaggg tcaccttcgc tttgtttgaa
     7141 ctcatcacga atatcctgcc gtgacatacg cagctttttc aggtggctga atatttggaa
     7201 aaagacgtca aatcccacca ttggaatgac accaagcacc accagcagtg cgcatagccc
     7261 taccaaatcc atcgcattac ccatggcggt aatcggagac tcggccatca agcgcatcat
     7321 ctgcggccag tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa
     7381 aattgcttta agcaactccg cgccagtctg agccgagaac atccgtttaa tgcccggtag
     7441 cgggttgagt ttggaaaact tcggctgcaa ggatttgccg ctaaatacca gccctcccag
     7501 catgaccgga gaaatgagcg ccaccagcac cacgccgcta atcagcggca gcagcgccag
     7561 catggcttct ctgatcagca gaataatctg cccgaggatc agattcgggt cattgataat
     7621 actgtgatca aaatgcagcc cagcggagag catgcccgac aatcgacggg ccagcgacac
     7681 accgccaaac cagataacac taacgcccac cagcaaaatc agcagtgagg tcagttcacg
     7741 ggaacgcggg atttgcccct cttcccgcgc tttttctagt cggtgaggtg tgggggcttc
     7801 tgttttgtcg tcgctctcgt cagacacgtc gccaatcctg gaaagagtat taagcgtgaa
     7861 tgatgccaga gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag
     7921 ggcttttgct gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt
     7981 atcagaccgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc aaaatccaag
     8041 actatccaac aaatcgtcca cctgatcctg actggctacc acaccggctt tgctggtatc
     8101 gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg
     8161 gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat
     8221 ccgcttaatg acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag
     8281 ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt
     8341 taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg
     8401 ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact
     8461 gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg
     8521 cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa
     8581 actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg
     8641 ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct
     8701 caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc
     8761 cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca
     8821 ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc
     8881 catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact
     8941 tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttca
     9001 gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt
     9061 ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt
     9121 tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt
     9181 gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca
     9241 tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag
     9301 gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac
     9361 cgaaggccga tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc
     9421 actacgcgac agctccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac
     9481 acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc
     9541 aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag
     9601 cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc
     9661 aatcagtttt tcagaactca acaacggacc cgccttcagc gttgtcggtg ctgacaatgg
     9721 cttatgtgct gcaaggctcg cctttgctgc tgtacgcacc ttttcagcaa tcatttcgct
     9781 atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctatcgcccc
     9841 cagctccagc gcgcgcagcg tgacttctga ccctttgcca gtcagggaag aaaccatcac
     9901 tacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg
     9961 catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcgac
    10021 cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt
    10081 catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatctt
    10141 taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg
    10201 ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag
    10261 cggcgcaaaa tctcctgctg ggtattttga tcgaagtaga tcatgacgtt acgacagaag
    10321 atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtagattcag cggggcaaaa
    10381 tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct
    10441 cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa
    10501 ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag
    10561 cgtccgggcg cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg
    10621 ccggtcgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc
    10681 gcgagcagag ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa
    10741 ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga
    10801 ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg
    10861 cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga
    10921 aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca
    10981 cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt
    11041 tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca
    11101 cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct
    11161 accagcgagg cgttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc
    11221 tcaatccccc gctgttgttc ttccgacgca gaggcaattt ctcccataat gtcgttcacg
    11281 cgggtcaccg aactgacaat atcgatcatg gtcgcggcgg cgttattcac cagtttcgaa
    11341 ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc
    11401 gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc
    11461 tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca
    11521 attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc
    11581 atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt
    11641 tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat
    11701 tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag
    11761 agatcgttat tgcctgcgac aatctccgcg ataccaatgt gcatctcctg gcttccctta
    11821 cgcacatcac ttaccgtccc acgcaaagcc tgctgcatgg tcttcagact ggcaaaaatg
    11881 gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg
    11941 ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac
    12001 agcgcactgc tgatgtagat tgccgcaaca ataatcatgc tgataaacac cagtgccgaa
    12061 atctgatagt tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccatc
    12121 tgccaggcct caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg
    12181 aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag
    12241 cgggcaaaac ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc
    12301 atcgccataa aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc
    12361 atcaacgttt taatgtcatc cgccggatag ctaagtgccg tcagagtgcc cgctttgttc
    12421 agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga
    12481 ttgctctgct cgacctgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg
    12541 ccgttactgc caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa
    12601 attcgaatac gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaat
    12661 actcgtcaaa atgtttccca gtttggatct tgttcagcaa ttcgcagtcg tggctgagcc
    12721 ggtggttgct cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct
    12781 gccagacgga acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagcg
    12841 gcggcggcag ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc
    12901 gaaaccgcca atgcgacttg atcgatgcca cgactctgtt catccgatgc cgatgcaatc
    12961 tcgcccataa tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg
    13021 gcgctttcga ccagcaccga accggtatca acgcgtgaga cggagtcttc aatgagggct
    13081 ttgatctctt ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc acccgccacc
    13141 acggcaaaac cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg
    13201 aggatattag tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc
    13261 gaactatcgg cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc
    13321 tgggcggtgt cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt
    13381 tgcttcactg tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc
    13441 tgctgttcag tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca
    13501 tagatggcat ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata
    13561 tgtgaaacgc tctgcgccag gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc
    13621 gccaggttac caccggcgat ttcgcgaatg tgagcaataa tttttgccag cggagtaagc
    13681 aacatacggc gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata
    13741 accgccagtt gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga
    13801 tacagttttt cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt
    13861 ccctgggttg gctgagcgaa ataagctcca gtattgccat aa
//
LOCUS       DAPHDX010000116        13861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPHDX010000116 DAPHDX010000000
VERSION     DAPHDX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.117"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(263..763)
                     /locus_tag="RH058_003454"
     CDS             complement(263..763)
                     /locus_tag="RH058_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV3139000.1"
                     /translation="MNNTDSLEKIIKHQQNNDPKYPCQEHLLIQLCIRVNKKIQDNIS
                     SLLSGYGINYSTYMVLTILSTADNHCLSPSKISRELQFTRTNITRITDFLEKAGYIER
                     TDSREDRRAKKISLTSDGLSFIQNVNIAQSLHLKEIWSPLTHAECELFEDINKKLLTH
                     IADVCP"
     gene            complement(1016..1225)
                     /locus_tag="RH058_003455"
                     /pseudo
     CDS             complement(1016..1225)
                     /locus_tag="RH058_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1393..1909
                     /locus_tag="RH058_003456"
                     /pseudo
     CDS             1393..1909
                     /locus_tag="RH058_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1800..2147
                     /gene="tnpB"
                     /locus_tag="RH058_003457"
     CDS             1800..2147
                     /gene="tnpB"
                     /locus_tag="RH058_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3139001.1"
                     /translation="MITLPAGSRIWLVAGITDMRNGFNGLAAKVQNTLKDDPISGHLF
                     IFRGRRGRLVKLLWADSGGLCIYMKRLERGTFIWPVAREGKVYLTPAQLAMLLEGIVW
                     MQPGRSKSNSLRI"
     gene            2257..3776
                     /locus_tag="RH058_003458"
                     /pseudo
     CDS             2257..3776
                     /locus_tag="RH058_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3786..4993)
                     /locus_tag="RH058_003459"
                     /pseudo
     CDS             complement(3786..4993)
                     /locus_tag="RH058_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5117..5953)
                     /locus_tag="RH058_003460"
                     /pseudo
     CDS             complement(5117..5953)
                     /locus_tag="RH058_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            5946..6596
                     /locus_tag="RH058_003461"
                     /pseudo
     CDS             5946..6596
                     /locus_tag="RH058_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6774..7055)
                     /locus_tag="RH058_003462"
     CDS             complement(6774..7055)
                     /locus_tag="RH058_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139002.1"
                     /translation="MSESVKLSFYRVHECGYYLWGNNTPVFGSLQELLTDLHFWSTDK
                     SIENTKLYEPQADSDYLGTYLFNINRSGDYWLVTIWNEVPSTKRELLLL"
     gene            complement(7241..12751)
                     /locus_tag="RH058_003463"
     CDS             complement(7241..12751)
                     /locus_tag="RH058_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3139003.1"
                     /translation="MINKNKVTERSGIHDTVKSLSENLRKYIEAQYHIRDEGLIAERR
                     ALLQQNETIAQAPYIEATPIYEPGAPYSELPIPEAASNVLTQLSELGIGLYQRPYKHQ
                     SQALESFLGENASDLVIATGTGSGKTESFLMPIIGKLAIESSERPKSASLPGCRAILL
                     YPMNALVNDQLARIRRLFGDSEASKILRSGRCAPVRFGAYTGRTPYPGRRSSRRDELF
                     IKPLFDEFYNKLANNAPVRAELNRIGRWPSKDLDAFYGQSASQAKTYVSGKKTGKQFV
                     LNNWGERLITQPEDRELMTRHEIQNRCPELLITNYSMLEYMLMRPIERNIFEQTKEWL
                     KADEMNELILVLDEAHMYRGAGGAEVALLIRRLCARLDIPRERMRCILTSASLGSIED
                     GERFAQDLTGLSPTSSRKFRIIEGTRESRPESQIVTSKEANALAEFDLNSFQCVAEDL
                     ESAYAAIESLAERMGWQKPMIKDHSTLRNWLFDNLTGFGPIETLIEIVSGKAVKLNIL
                     SENLFPDSPQQIAERATDALLALGCYAQRASDGRVLIPTRMHLFYRGLPGLYACIDPD
                     CNQRLGNHSGPTILGRLYTKPLDQCKCASKGRVYELFTHRDCGAAFIRGYVSSEMDFV
                     WHQPNGPLSEDEDIDLVPIDILVEETPHVHSDYQDRWLHIATGRLSKQCQDEDSGYRK
                     VFIPDRVKSGSEITFDECPVCMRKTRSAQNEPSKIMDHVTKGEAPFTTLVRTQISHQP
                     ASRPIDGKHPNGGKKVLIFSDGRQKAARLARDIPRDIELDLFRQSIALACSKLKDINR
                     EPKPTSVLYLAFLSVLSEHDLLIFDGEDSRKVVMARDEFYRDYNSDLAQAFDDSFSPQ
                     ESPSRYKIALLKLLCSNYYSLSGTTVGFVEPSQLKSKKMWEDVQSKKLNIESKDVHAL
                     AVAWIDTLLTEFAFDESIDSTLRIKAAGFYKPTWGSQGRFGKALRKTLIQYPAMGELY
                     VEVLEEIFRTHLTLGKDGVYFLAPNALRLKIDLLHVWKQCNDCTALMPFALEHSTCLA
                     CGSNSVKTVEPSESSYINARKGFWRSPVEEVLVSNSRLLNLSVEEHTAQLSHRDRASV
                     HATTELYELRFQDVLINDNDKPIDVLSCTTTMEVGVDIGSLVAVALRNVPPQRENYQQ
                     RAGRAGRRGASVSTVVTYSQNGPHDSYYFLNPERIVAGSPRTPEVKVNNPKIARRHVH
                     SFLVQTFFHELMEQGIYNPAEKTAILEKALGTTRDFFHGAKDTGLNLDSFNNWVKNRI
                     LSTNGDLRTSVAAWLPPVLETGGLSASDWFAKVAEEFLNTLHGLAEIVPQTAVLVDEE
                     NEDDEQTSGGMKFAQEELLEFLFYHGLLPSYAFPTSLCSFLVEKIVKNIRGSFEVRTV
                     QQPQQSISQALSEYAPGRLIVIDRKTYRSGGVFSNALKGELNRARKLFNNPKKFIHCD
                     KCSFVRDPHNNQNSENTCPICGGILKVEIMIQPEVFGPENAKELNEDDREQEITYVTA
                     AQYPQPVDPEDFKFNNGGAHIVFTHAIDQKLVTVNRGKNEGESSGFSVCCECGAASVY
                     DSYSPAKGAHERPYKYIATKETPRLCSGEYKRVFLGHDFRTDLLLLRITVGSPLVTDT
                     SNAIVLRMYEDALYTIAEALRLAASRHKQLDLDPAEFGSGFRILPTIEEDTQALDLFL
                     YDTLSGGAGYAEVAAANLDDILTATLALLESCECDTSCTDCLNHFHNQHIQSRLDRKL
                     GASLLRYALYGMVPRCASPDIQVEKLSQLRASLELDGFQCIIKGTQEAPMIVSLNDRS
                     IAVGSYPGLIDRPDFQHDVYKSKHTNAHIAFNEYLLRSNLPQSHQNIRKMLR"
     gene            complement(12748..13791)
                     /locus_tag="RH058_003464"
     CDS             complement(12748..13791)
                     /locus_tag="RH058_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139004.1"
                     /translation="MEWRAVSRDKALDMLSTALNCRFDDEGLRISAVSECLRSVLYQY
                     SISETEEARQTVTSLRLTSAVRRKLVPLWPDIADIDNAIHPGIMSILNSLAELGDMIK
                     LEGGNWLTAPPHAVRIDNKMAVFFGGEPSCTFSTGVVAKSAGRVRLVEEKVCTGSVEI
                     WDANEWIGAPAEGNEEWSSRLLSGTISGFIDAPGNMSETTAYVRGKWLHLSELSFNKK
                     QIYLCRMSVDNHFSYYLGEIEAGRLCRMNSLESSDDVRRLRFFLDTKDNCPLKVRIKI
                     SNGLARLRLTRRLPRRETKVLLLGWRESGFENEHSGITHHVFPEEILPIVRSAFEGLG
                     IIWINEFTRRNEI"
BASE COUNT         3624 a         3306 c         3141 g         3790 t
ORIGIN      
        1 cgcgttaccc catatgagat aagctcaatc attacgttat cttgtatcag taagttctac
       61 ggtgaatcat gctgatattt gcgggttagg caccgcatca ttcacaagaa ccgtcgtatg
      121 agtttgtcat tttgctgcat tgtgacctaa accagtagtt ttcttgtatc gctgtttccg
      181 cctgtagtta tgtcgctctt atgcctgctt attatatgat tcattgcttg aagctcttca
      241 gtttcctcaa actcttcatg ctttagggac acacatcagc aatatgcgtc agtaattttt
      301 tattaatgtc ttcaaacagt tcacactctg catgggtcag cgggctccag atttctttca
      361 ggtgcagact ttgtgcaata ttgacgttct gaataaagga cagaccatct gatgtcagac
      421 tgattttttt agcacggcga tcctctctgc tatcagttct ttctatatac cctgcttttt
      481 ctaaaaaatc tgtaatgcgg gtaatgtttg ttctggtaaa ctggagttct ctgcttatct
      541 ttgagggcga aagacaatga ttatctgctg tggataatat ggtaagaacc atatatgttg
      601 aataatttat tccatatcca ctgagtaaac tcgatatatt atcctgtatt tttttgttta
      661 cgcggataca gagttgtatc aacagatgct cctggcaggg gtattttgga tcgttgtttt
      721 gttgatgttt gattattttt tctaatgagt ctgtgttgtt catataatca tttccatttg
      781 ctgaaatact ttgcctttat acaaaagtgc atcagacatg cggtaagcat ctacttttca
      841 caacaagtta tgtctgccag atatctgtat gaatgagaca accaggcaga aaggaaggat
      901 aacttcattc gcataaaaaa acataaatgt ttgtagtggt caacaaaaaa tgatcacgat
      961 tttaaatttt tttccagtat ctattttcca attcgtttgg tagtaacctg ccattttatt
     1021 cgtgaggcct gagtgcacta taatacccaa cgatatagtc cgttatgact tgaactgcat
     1081 cgctgaagct tacgtacccc accactggta accattcgtt ctttagactc ctgaagatgc
     1141 gttccattgg gctgttatcc cagcagtttc cctatcggct gatactttgc tttatcctgt
     1201 aacaccagag aagttgttga tatttcagtt cagtatactg acctcttgcc tggcttgatc
     1261 aactggtaaa gtacttcacc gttgagcttc ccgtggttga ttttacgtaa gcgacccgtc
     1321 agagacgtct tcaggcagtc gagtgaaaac gctaacttag acgcccatct gaaacagacg
     1381 gacatctaag ttatggaatc tgaaacccgg gcctggcgga ccgaaccccg ccgcgtttat
     1441 tccagcgaat tcaaactcag gctggtcgaa ctggctaccc gaccggacgc caatgttgcg
     1501 gatattgccc gtacgcacgg ggtcaacgat aacgtccttt ttaaatggat ccgtctgtgg
     1561 cgtaaagaag gccgggtatt ccgccggctg ccagctcccg ttccatctgt atcccctcct
     1621 gcggcatttc cggtcgaaat tgtacctgac tccaccaccc ggcagtctcc ggctctgtct
     1681 ggtcagggaa cggggtcaac ctgctcgctg acgcttcgcc acggcaccct cacactcgac
     1741 aatcctgacc cggagataca gctccggatc gtccgcgaac tcgccggcag gagcacagta
     1801 tgattacgct tccggctggc agccgtatct ggctcgtggc cggcatcacc gacatgagaa
     1861 acggcttcaa cggtctggcc gcaaaggtcc agaacaccct gaaggatgac ccgatctccg
     1921 gccatctctt catcttccgg ggacgcaggg gcaggctggt aaaactcctg tgggcagaca
     1981 gcggagggct ctgcatctat atgaaaagac ttgaacgggg aacgttcatc tggccggtcg
     2041 cccgggaggg aaaggtgtat ctgaccccgg cacagcttgc catgctgctg gaggggatag
     2101 tctggatgca accgggaagg tcgaaaagta atagtctgcg gatctgaatc cctttacctg
     2161 ggcaggatga aaaatatccc gcttcctgat aatatcgacc agctgaaagc cctgttgcgt
     2221 cagcagcagg ttgctgtgta acagctaaat gttaccatga aaaagcagca ggcccggctg
     2281 cgccagtacg ccggacaggt tgccgactac gaacaggaaa tcgaacggct gaaggcacag
     2341 cttgataagt tgataagctg cggcggatgc tgttcgggca gagttcagag aaaagccgca
     2401 ataaactgga aaataaaatc cgccaggcag agaagcggct ggctgagctg gaaaaccgtc
     2461 ttgcagcggc aaaacgctgt ctgcaggacg atgagcccac tgtcagtacg gttgagcagg
     2521 cagagccccc ggtggcggtc gccccacagg accggcctgc acgccgctcc cgtcgtcaga
     2581 aactgccggc agacctgcca cgtgaaaccc ggcgcatcac cccgacagtg actgtctgcc
     2641 cgaaatgcgg cggcgacctg aaggtgatga gtgaaaccgt ctcagagcaa ctggagataa
     2701 tccgcagcgc ctttaaggtc atcgaaacgg tgcgcccgaa gctggcctgc agccgctgcg
     2761 atgtgatagt ccaggcccca ctcctcccga aacctctgac ccgcagtgtg gccggtgcgg
     2821 gcctgctggc ccgcatcctg gtgagtaaat actgtgacca taccccgctg catcgtcagt
     2881 ccgtcatctt cagccgccag ggtgtggaac tgagccgaaa cacgctggtc cggtgggtgg
     2941 cgatgaccgc agaacgactc agtcccctgc acgatgcgct gaaccgctac gtgatggaga
     3001 gcagcaaaca gcacacggac gataccccgg tgaaagtgct ggagccgggg agcggtaaaa
     3061 cccggacggg ccggctgtgg gtgtatgtgc gcgatgaccg gaacagcggt tcagcggctc
     3121 cgccagcggc gtggttcgct tacagtacag atcgtcgggg ctgtcacccg caggggcatc
     3181 tggcggagtg gaaaggtatc ctgcaggcag atgcgtttgc cggttacgat gcgctttatg
     3241 agtcagggct gatcagggaa gccgcctgca tggcgcatgc aaggagaaag atccatgatg
     3301 agcatgttcg ccggccgaca gcgatgacaa cggaggcgct gaagcgtata gccgggctgt
     3361 atgcggtgga agcgacaatc cggggtcaac cggcagagaa acggctggca gaacggcagg
     3421 agaaaagcgt cccgcagatg aaggcgttgt tcgcgtggct gacagaacag atggagacgt
     3481 tgtcggtgca tgcagagctg gggaaagcgt tcagctatat gctgaaccac cgtgaagcgc
     3541 agtcgctctg ttgtgaggac gggcgtgtgg aactggacaa taatatcgca gaaaacacac
     3601 tgcgttgtgt cgccctatgg cgacgcaact gtctattctt cggctcagac acgggaggtc
     3661 agagcgcagc gatcctgtac agcctgctgg cgacatgccg gctgaatgga gtggatccgg
     3721 aaagctggtt gcgtgaggta atcagctgta tcgacccagt ttaccattct gtgtaaatgc
     3781 ctttctcaga agtgaccgtc aaggcggtca ccgaatttga taataaagcg actcatggct
     3841 atccgccagt cctgaagtgg catcgtccat ttctgtgagg ctgactggat agccagccac
     3901 accacttttt tcaccgattc atctgtctgg aacactttcc gtttctttat cgcatgtctg
     3961 atgacgctat tcagcgattc gattgcgtta gtggtgtaga taaccttgcg gatcgcctcc
     4021 gggtacacga agaaggtggc aacattcggc cagtttgccc gccaactgcg acttatctgc
     4081 gggtacagtg catcacattc cttcccgaac gcatccagcg cctgaaacgc gccttcttcc
     4141 gtggctgcgc ggtaaacctg tttcaggtca cgggttaccg ctttatagtc tttccacgcc
     4201 acgaaccgca ggctgttgcg taccatgtgt actatgcaca actggatctg cgtctccggg
     4261 tagacggcat taatcgcttc cgggaagcct ttcaggccgt ccacgcaggc gataaggata
     4321 tcgttcaggc cgcgattttt cagttccgtc agtacattca gccagaactt tgcaccttca
     4381 ttttcggcca gccacatacc tggcagctct ttctgaccat caaggttaat acccagagcc
     4441 aggaacaccg ctttgttgat aacccggctg tcctgccgta ctttcaggac aatacagtca
     4501 agataaataa tgggataaat cgcatccaga ggacggtttt gccattcaac cacctggtcg
     4561 attacggcat cggtgacttt cgatatcagc gccggagaga cgtctgcatc gtacatttct
     4621 ttgaagactg aggtaatttc gcgggtagtc atccctctgg catacagccc aagatctggt
     4681 tgtccatgcc ggtaatgcgg gtctgatttt tcttcaccag ttgtggttcg aaggaaccat
     4741 cacgatcacg tggagtacgt aattccatcg gcccgtcggt agtggtgacg gttttcgtgg
     4801 aatagccatt acgggagttg gttcccgatt tgggctgatt tttttcatgt cccagatggt
     4861 gagtcagttc agcgttgaga gcagcctcaa cactgatttt cttgagcagg cgatcgaact
     4921 gactgagatc ttcaggggtt ttgagatttt tggccagttc gttagccaga gcctgcaatt
     4981 gtttgtcgtc cataatttaa ccttcatttg atgttgaatt gaacatatca aaatcaggca
     5041 attacacaaa tctatgtaca ggctcaaagt tatcgcctta tgtaactgct cttgaaagaa
     5101 tcaatgaaca aattgagagc cagtttttcc ggcctacggc cactccccgt aaggtgttct
     5161 ctgcaatgtt gttgtcgatt tccacccagc cattactgca gtagacgttc agggcctccc
     5221 actgtttcag caggtatgcg aacgcttttg ccgtatccga gtgacgcgac agtgttttca
     5281 tctgagtctg tatccagtca tacagtgact gcatcagcgg cgcagctctg gcttttcttg
     5341 ccgccagatg ctgttctgct gaacatcccc ggacttctgc ctcgatgaca tacagttcac
     5401 cgatacgctg cagggcttcc gtggtgatgt cggttggcac tcttgcatgc acatcgtgga
     5461 tttttctccg ggcatgagcc atacacgcgg cttccgttat tctgccggat tcgtataaca
     5521 cccggtaacc accgtaagca tcggcctgaa acacaccgct gtagccggcc agatgatttt
     5581 gtggatggat acctttccgg tccggtgagt acgcgaacca gaccgctggg ggcatctgtg
     5641 aaccggcatt acggtcatca cggacgtagg cccacagccg ggcagtcctt gttttaccac
     5701 tgcccggctc ctgtaccgga acggggatat catcggcatg gactttaccg ggcaccagca
     5761 catactggcg caggacgtca tacaacggct ccagcagttc agcaacagca cctgtccagc
     5821 gccccagtgt ggcacggctc agctccactc cctgacgacg atatatttct gactggcggt
     5881 ataacggcag atggtctgca tattttccag cgacaacatg ggccagaagc cccgctccgg
     5941 cataactgcg tgctcagggt taccacttta acgtaaaaac cgtgtcggca agcctgcacc
     6001 gtcaggggct gagggcaaag gcctcccgga agttcagtcc ggtcagttac cgcgcacatg
     6061 gcctgcctgt gtcagaaaat ctgttggagc aggattttta cgccagtggt ccgaaccaga
     6121 agtgggcggg agcatcacgt acttacgtac gtgatgaagg ctggctgtat ctggcagtgg
     6181 tcattgacct gtggtcacgt gctgttattg gctggtccat gtcgccacgc atgacggcgc
     6241 aactggcctg cgatgccctg cagatggcgc tgtggtggcg taagagaccc agaaacgtta
     6301 tcgttcactc ggaccgcgga agccagtact gttcagcaga ttatcaggcg ctgctgaagc
     6361 ggcataatct gcgtggcagt atgagcgcaa agggctgctg ctacgataat gcctgcgtgg
     6421 aaagcttctt tcattcactg aaagtggaat gtctccatgg agaacacttt atcagccggg
     6481 aaataatgcg ggcaacggtg tttaattata tcgaatgtga ttacaatcgc tggcgacggc
     6541 acagttggtg tggcggtctc agtccggaac aatttgaaaa caagaacctc gcttagagca
     6601 gtgtccatat tacgagggga ggatcagttg ttctgtttga gttctatttt tgaatcgcac
     6661 actggcaaat aatccataac taggtaaaat ccaaaaataa gtcgcgtaac cgggaataga
     6721 gttatcttgt atttggttca taactacatc gggcgcagta acacttgaat gcctcataac
     6781 agaagcaact cccgcttggt tgatggaact tcattccaaa tagtaactag ccaataatcg
     6841 cctgacctat tgatattaaa taagtaagta cctaaataat cactatctgc ttgtggttca
     6901 taaagttttg tgttttctat actcttatcc gtactccaaa agtgaagatc tgtcaggagc
     6961 tcctgaagtg agccaaaaac aggagtatta ttaccccata agtagtatcc acattcatgt
     7021 actcgataaa aactaagttt aaccgattcg ctcattggtc agaagttccc ttaccttatt
     7081 attctcaatg ggcataattt tacattgtgc aatagttgct ggcgagttaa atggtcacat
     7141 tttctcccat acgtacattt atggcttcag ttaaagcccg ctcacgcact gaaaaccttg
     7201 agtgccctac agggctttag ggcactcaat actgctgcta tcagcgcaac atttttctaa
     7261 tattttgatg cgattgtggc agatttgaac gaagaagata ttcattaaag gctatatgag
     7321 cattagtatg ctttgactta tatacgtcgt gttgaaagtc gggtcgatca ataagaccag
     7381 gataacttcc cactgcaata gaacggtcat tcaaactcac aatcataggt gcctcctgag
     7441 ttcccttaat tatgcattga aaaccatcca attccagact tgccctcaat tgagacaatt
     7501 tttctacctg aatatcaggt gaagcacaac gaggaaccat tccgtatagt gcataacgaa
     7561 gtaaagatgc acctagtttc ctatcgagac ggctttgtat atgctggttg tggaaatgat
     7621 tgagacaatc tgtacaggag gtatcgcact cacagctttc taacaatgcg agtgttgcag
     7681 taagaatgtc atctagattc gctgctgcta cttccgcata acccgcaccg ccggataaag
     7741 tatcataaag gaagagatcc aatgcctgag tatcttcctc tatagtgggt aaaattctga
     7801 aaccagagcc aaactcagca ggatcaagat ccagttgttt atggcgacta gctgcaagcc
     7861 ttagtgcttc cgctattgta tataatgcat cttcatacat ccgtaaaacg atagcatttg
     7921 aagtatcagt tacaagcgga gacccaacgg ttattcgtaa taaaagcaaa tcagtacgga
     7981 aatcatgtcc gagaaaaacg cgtttatact cgccagagca taagcgaggc gtttccttag
     8041 ttgctatata tttatacggt ctttcatgtg ccccctttgc cggtgagtag gaatcataaa
     8101 cggaggccgc accacattcg caacatactg aaaaaccact ggactccccc tcatttttcc
     8161 ctcggttcac cgtcaccagt ttctgatcta ttgcgtgagt aaaaacaata tgagcacctc
     8221 cattattgaa cttaaaatct tcaggatcaa caggttgtgg atattgtgcc gctgttacat
     8281 aggtgatttc ttgctctctg tcgtcctcat taagttcctt ggcattttca ggtccaaaga
     8341 cttcgggctg aatcattatt tctactttta gaatgccacc acagatcgga caagtatttt
     8401 cgctattctg attattatga ggatcgcgga caaaagagca cttatcgcaa tgaataaact
     8461 ttttgggatt attgaaaagc tttcttgccc ggtttagttc gcctttcaat gcattagaaa
     8521 aaacaccacc agagcgatag gttttcctat caataacaat caaacgtccc ggggcatatt
     8581 cactcagagc ctgagaaatt gattgctgag gctgttgtac tgttcgcacc tcaaaagaac
     8641 ctctaatatt ctttacaatt ttttctacca agaaactaca gaggcttgta ggaaatgcat
     8701 aacttggtaa taaaccatgg taaaacagga actcaagtaa ttcttcttgt gcaaatttca
     8761 ttccaccaga agtctgctca tcatcttcat tttcctcatc aacaagaacg gcagtttgtg
     8821 gaacaatttc agccagccca tggagtgtat ttaaaaattc ctctgctacc ttagcaaacc
     8881 agtcactggc agaaagccct ccagtttcaa gaacaggagg aagccatgct gcaacacttg
     8941 ttctcaaatc accattagta gatagaatac ggtttttaac ccaattatta aagctatcga
     9001 gatttaggcc agtatctttt gctccatgaa aaaaatctcg tgtagtacca agtgctttct
     9061 caagtatggc agttttctct gcgggattat aaattccttg ttccattaac tcgtgaaaaa
     9121 aggtctgaac taaaaaagaa tgaacgtgtc ttctggctat tttgggatta tttactttca
     9181 cttcaggtgt acgaggagaa cctgcaacaa tgcgttcagg attaaggaaa taataactat
     9241 catgagggcc attttgagaa tatgtaacca ccgttgaaac agatgcgcca cggcggcctg
     9301 ctcgcccagc acgttgctga taattttctc gttgcggagg gacgtttctt aaagcaacag
     9361 caaccagaga tccaatatca acccccactt ccatcgtcgt cgtacaacta agtacatcaa
     9421 tgggcttgtc gttatcatta ataagaacat cttggaatct cagttcgtag agttctgtag
     9481 tggcatgaac gctggcccta tctctatgtg agagttgagc agtatgctct tcaacgctaa
     9541 ggtttagaag ccgcgaattt gaaaccaaaa cttcttctac cggcgaacgc cagaatcctt
     9601 ttcgtgcatt aatatagctg ctttccgacg gctcgactgt tttgacactg ttactaccac
     9661 aagcaaggca agtagaatgt tctaaagcaa atggcattag tgctgtgcag tcattacatt
     9721 gtttccagac atgcaagaga tctattttca gacgtagtgc atttggagca agaaagtaga
     9781 caccatcttt tcctaatgtc agatgagtac gaaaaatctc ctccaaaact tccacataaa
     9841 gctcccccat agcaggatac tgtatcaggg ttttcctaag agcttttcca aaccgtcctt
     9901 gactacccca agtgggtttg tagaatccag ctgctttgat tcgtagtgtc gaatcaatag
     9961 attcatcaaa agcaaattca gtgagtaagg tatcaatcca agcaacagct aaagcatgaa
    10021 catccttgct ctcaatattg agtttcttgg actgcacatc ttcccacatt ttttttgatt
    10081 taagctgcga tggttcaaca aaaccaactg ttgttccgga aagagaatag taattgctac
    10141 ataaaagttt aagcaacgct attttatatc gtgacggtga ctcttggggg ctgaagctat
    10201 catcaaaagc ttgagccaga tcgctattat aatcacgata aaattcatca cgggccatta
    10261 caacttttcg tgaatcttcc ccatcaaaaa taagcaagtc atgttcagaa aggactgata
    10321 ggaaagcaag gtaaagtact gatgttggtt tgggttcccg attgatatct ttcagtttag
    10381 aacaggcgag agcaatggat tgccgaaaca aatcaagctc aatatctcta ggaatatcac
    10441 gtgcaagccg agctgctttt tgtcggccat cagaaaaaat aagtactttt tttcccccat
    10501 tgggatgttt accatcaata ggacgactcg ctggctggtg agatatctgt gtacgtacta
    10561 acgttgtaaa aggtgcttcc ccttttgtaa catgatccat aattttagac ggttcattct
    10621 gagcacttct tgtcttacgc atacaaacag ggcattcatc aaatgtaatt tctgatccag
    10681 acttaactcg gtcaggtata aagactttac gataaccaga atcctcatct tgacactgtt
    10741 tagaaaggcg tcctgttgct atatgtagcc atctgtcctg gtaatcacta tgtacatgag
    10801 gtgtttcctc gaccaatata tctatgggaa caagatcgat atcctcatct tctgataatg
    10861 gtccgttcgg ctggtgccat acaaagtcca tttcggaact aacgtatcca cgaataaaag
    10921 ccgcaccgca gtcacggtgg gtaaataatt cgtagactcg cccttttgaa gcgcatttac
    10981 attgatccag tggtttcgta taaaggcggc caagtatagt tggcccgcta tggttaccca
    11041 aacgttgatt acaatcggga tctatacagg cataaagacc tggtaatccc cgataaaaaa
    11101 gatgcatgcg agttggaata agcactctgc catcggatgc cctctgagca tagcaaccca
    11161 atgcgagtaa tgcatctgtt gctcgctctg cgatttgctg tggagagtct ggaaaaaggt
    11221 tttcactcaa gatatttagc ttaaccgctt tacctgaaac tatttcaata agcgtttcaa
    11281 taggaccaaa accagtcaaa ttatcaaata accaattacg tagtgtacta tgatctttta
    11341 tcatcggctt ttgccagccc attcgttcgg caagagactc tattgctgca tatgcagatt
    11401 caagatcctc agctacgcac tgaaatgaat ttaggtcgaa ttcagccagt gcattagctt
    11461 ctttactggt aacaatttgt gactcaggac gcgattccct tgtaccctca ataattcgaa
    11521 atttcctcga agaggttggt gataagccag ttaagtcttg ggcaaaacgt tctccatcct
    11581 caatggaccc tagactagca ctggtaagga tgcagcgcat acgttcccgg ggaatatcca
    11641 accgagcaca gaggcgacgt attaaaaggg ctacctctgc tccccctgct cctctataca
    11701 tatgcgcttc atcaagcact aagataagct cattcatctc atcagctttg agccattcct
    11761 tagtctgctc aaaaatatta cgctcgatag gtcgcatcag catatactca agcatggagt
    11821 agttcgttat cagtaattct ggacagcgat tctgtatttc atgccgggtc attagctcac
    11881 gatcctcagg ctgggtaatt agcctctccc cccaattgtt caaaacaaat tgcttacccg
    11941 tttttttgcc tgagacgtag gttttagcct gagatgcgct ttgcccataa aaagcatcaa
    12001 gatctttact tggccagcga ccaatgcggt tcagttccgc acgtacgggg gcgttatttg
    12061 cgagtttatt gtaaaactca tcgaaaaggg gtttgataaa aagctcgtct cgtctagagc
    12121 tacgacgacc agggtaaggc gttcttcccg tataagcgcc aaagcgtaca ggggcacatc
    12181 ttccagatct cagtatttta gaggcttcag aatcaccaaa aagacgtctg atacgagcaa
    12241 gttgatcgtt aactaatgca ttcattggat ataataaaat tgctctacaa cctggaaggg
    12301 atgcagattt aggtctctcg gaagattcaa tcgccaattt tccaataatt ggcattagaa
    12361 agctttcagt cttaccggag cctgtacctg ttgcaatgac cagatcagaa gcgttttcgc
    12421 caagaaatga ctcaagtgcc tgtgattggt gtttataggg gcgttgatag aggccaattc
    12481 caagttctga tagttgagtt agcacattac ttgctgcttc gggaataggc aattcactgt
    12541 atggcgcacc aggttcataa attggggttg cttctatata aggagcttga gcaatagttt
    12601 cattttgctg taaaagcgct cgtcgctcag caattaaccc ttcatcccgg atatgatatt
    12661 gtgcctcaat gtattttctc agattttcac taaggctttt cacggtatca tgtatacctg
    12721 aacgttcagt tactttattt ttattaatca tatttcattc cgtcgcgtga attcgttaat
    12781 ccaaataata ccaagccctt caaaagcgct acgcactatg ggtaatattt cctcggggaa
    12841 tacatggtgt gttattcctg aatgttcatt ttcaaaacct gattctctcc agcctagcag
    12901 gagtaccttc gtttctcgtc ttggtaatct tctggttaat cttaatcttg ctagcccatt
    12961 agatattttg atacggacct ttagcggaca attatctttt gtatcgagaa aaaaacgtaa
    13021 tcttctgaca tcatcagacg attctaacga attcattcta cataagcgtc cagcttcaat
    13081 ttctcctaaa taataggaaa agtgattatc aacggacatt ctgcataagt agatttgctt
    13141 tttattaaaa gaaagttctg acaaatggag ccattttccc cgcacatatg cagtcgtttc
    13201 actcatattg ccaggtgcat cgataaagcc ggaaatagtt ccagatagta gtctggatga
    13261 ccattcttca ttgccttctg ctggggcacc aatccactca tttgcatccc agatttcaac
    13321 acttccagta cacacttttt cttcaaccaa gcgaactctt ccagcagatt tagctaccac
    13381 gcccgttgaa aatgtacagg aaggctctcc accaaaaaaa acagccatct tattgtcaat
    13441 tcgtactgca tgtgggggag ctgttagcca attaccacct tctaacttaa tcatgtcacc
    13501 caattcagcc aagctgttca atatagacat aatgcccgga tgtatagcat tatcaatatc
    13561 agcaatgtct ggccataaag gtaccaattt tcgccttact gcactagtga gtcgaagcga
    13621 ggttacagtt tgcctagctt cttctgtttc agatatagaa tattgatata atacgctcct
    13681 taagcattct gaaactgctg aaattctcaa cccttcatca tcaaatcgac aatttaatgc
    13741 agttgataac atatccagtg ctttgtctcg tgatactgct ctccactcca ttaaccacct
    13801 cctcaagaag atatttttgc actataattt tttaattcac gattggcaat atcttctatc
    13861 t
//
LOCUS       DAPHDX010000117        13795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPHDX010000117 DAPHDX010000000
VERSION     DAPHDX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.118"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..1132
                     /gene="phoE"
                     /locus_tag="RH058_003465"
     CDS             77..1132
                     /gene="phoE"
                     /locus_tag="RH058_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV3139005.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1171..1572)
                     /gene="crl"
                     /locus_tag="RH058_003466"
     CDS             complement(1171..1572)
                     /gene="crl"
                     /locus_tag="RH058_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV3139006.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="RH058_003467"
     CDS             complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="RH058_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV3139007.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="RH058_003468"
     CDS             complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="RH058_003468"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV3139008.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3685..5142
                     /gene="pepD"
                     /locus_tag="RH058_003469"
     CDS             3685..5142
                     /gene="pepD"
                     /locus_tag="RH058_003469"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV3139009.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5199..5813)
                     /locus_tag="RH058_003470"
     CDS             complement(5199..5813)
                     /locus_tag="RH058_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV3139010.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5810..6949)
                     /locus_tag="RH058_003471"
     CDS             complement(5810..6949)
                     /locus_tag="RH058_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV3139011.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7195..7647)
                     /locus_tag="RH058_003472"
     CDS             complement(7195..7647)
                     /locus_tag="RH058_003472"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3139012.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7644..8699)
                     /gene="dinB"
                     /locus_tag="RH058_003473"
     CDS             complement(7644..8699)
                     /gene="dinB"
                     /locus_tag="RH058_003473"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV3139013.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8770..9693)
                     /gene="lafU"
                     /locus_tag="RH058_003474"
     CDS             complement(8770..9693)
                     /gene="lafU"
                     /locus_tag="RH058_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV3139014.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9696..10559)
                     /gene="motA"
                     /locus_tag="RH058_003475"
     CDS             complement(9696..10559)
                     /gene="motA"
                     /locus_tag="RH058_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3139015.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10572..11288)
                     /locus_tag="RH058_003476"
     CDS             complement(10572..11288)
                     /locus_tag="RH058_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDV3139016.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGILLAGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11308..11775)
                     /locus_tag="RH058_003477"
     CDS             complement(11308..11775)
                     /locus_tag="RH058_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDV3139017.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11783..12844)
                     /locus_tag="RH058_003478"
     CDS             complement(11783..12844)
                     /locus_tag="RH058_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDV3139018.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12841..13152)
                     /locus_tag="RH058_003479"
     CDS             complement(12841..13152)
                     /locus_tag="RH058_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDV3139019.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLCAVKARLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13157..13549)
                     /gene="fliS"
                     /locus_tag="RH058_003480"
     CDS             complement(13157..13549)
                     /gene="fliS"
                     /locus_tag="RH058_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV3139020.1"
                     /translation="MPKAEGRYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13527..13772)
                     /locus_tag="RH058_003481"
                     /pseudo
     CDS             complement(13527..13772)
                     /locus_tag="RH058_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
BASE COUNT         3274 a         3712 c         3386 g         3423 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga ctggctatgg
      301 ccgttgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac agcaaaaaac
      361 gcgtctcgct tttgccggat tgaagtataa agatttgggt tctttcgact atggccgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggggctacat attatttcaa
     1021 caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt
     1081 gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatgattatt
     1141 gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg gctcgtcacg
     1201 aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc
     1261 gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac
     1321 actcttccag tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga
     1381 ttcctgcgct tccagctcca tccaccagcc ccagaattca cgcacttccg gtgccggttt
     1441 cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc
     1501 acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact
     1561 cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaaattt
     1621 agcaaatttt taacacaagc gtttttcgat ccagccggtg atttcctgaa gacctttgtc
     1681 aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga
     1741 ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa
     1801 cattggcgtt gggcagcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag
     1861 ctccacgcgc aacgcgtcat cggaagcatc atgcatcccc aaacgactcg ccagaacgtc
     1921 aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac
     1981 cggaccaaga caggcaaccg ctttcagacg cggcgattca aggtatgcca gacgcacggc
     2041 aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac
     2101 gttaggcagc gcctttaaga cgtgctgatg caacaggctg gagtcctggg tgagcttcca
     2161 ttttgaagaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc
     2221 aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat
     2281 taatactgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc
     2341 accgccgggt acggtaaact ccatctgccg catcgtgccc ggcagacgct gggcggcctc
     2401 ttcataggcg cggtttgata aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg
     2461 aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac cactgagttg
     2521 cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata
     2581 aatccagttg ccaccacggt agccaatcac ggtatcgtat aaatcatcat cggtacgttc
     2641 agcatcgctc atcacaatac gtgcctggac gtcgaggatt tcacgtgggt caatgccgcg
     2701 ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag
     2761 agccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc
     2821 aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc
     2881 agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc
     2941 ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc
     3001 ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca tagtcatcaa
     3061 ccagcggacg accagccggt tttgcgaaga tagtcacaaa atgtgctttt ggatacattt
     3121 cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt
     3181 cgccatcgcc ttctgcgcgt tttagcactt taagctcgcg ctggttgtcg tgatcgtagc
     3241 tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg
     3301 gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc
     3361 ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc
     3421 tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag
     3481 gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt
     3541 ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc
     3601 aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc
     3661 gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg
     3721 tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc
     3781 gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta
     3841 ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc
     3901 ttacaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg
     3961 aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg
     4021 ggtgcagata acggcattgg tatggcgtct gcgctggcgg ttctggctga cgaaaacgtg
     4081 gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg
     4141 ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa
     4201 gaaggtgaaa tctacatggg ttgtgcgggg ggtatcgact tcacctccaa cctgcattta
     4261 gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa
     4321 ggcggtcact ccggcggtga aatccacgtt ggcctgggta atgccaacaa actactggtg
     4381 cgcttcctgg cgggtcatgc ggaagaactg gatctgcgcc ttatcgattt caacggcggc
     4441 acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa
     4501 gtcgacgtcc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca
     4561 gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg
     4621 attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc
     4681 cgcaattccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc
     4741 atgactgaca ataacgtaga aattcactgc ctgatccgtt cactgatcga cagcggtaaa
     4801 gactacgtgg tgagcatgct ggattcgctg ggtaaactag ctggcgcgaa aaccgaagcg
     4861 aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt
     4921 gaaacctatc agcgtctgtt caacaaaaca ccgaacatcc agattatcca cgcgggcctg
     4981 gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact
     5041 atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg
     5101 acactgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg
     5161 gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc
     5221 cctgtaaatg tccgtctcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc
     5281 gatttcagca ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat
     5341 cgtgccagcc gcttgttagc atgctgacta cgctctgact gaaccttcac gctaataccg
     5401 gatgccaaat gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc
     5461 cccgacgaac gcaacgtctc ataacggatt gcatccgatt gttcctgctc atcagcggta
     5521 aaacgcccaa tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga
     5581 caaatccact gaatagtgcc gcaccagctt tcgcttaatg cccatgcgtt atcgccatcc
     5641 agagaaacca gagctgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccaacacc
     5701 gttaccgcga cgtcttgtcg ggcagcttct ttaattagcc tgtccagcgc ctttttcact
     5761 gcgagacaac attcttccgg cccctgcgct gaggagagtt gtagcaagat catccttttt
     5821 tccctccact gtttttgagc gttagcaccg gacgcagccg cgcgacagga ataattaaac
     5881 cggcctgcac cagacattgc accacgcttt cagccgattt ataggcttgt ggcgcttctt
     5941 caaagatgag ttgtttatcg cgacaaatta cccgactgcc aagttcagta cgcgagagtt
     6001 gcgtcgccgt gtatttcgct gccagacgcc ctttacactc ggtgcgcccc catttacgcc
     6061 ctgccccatg cgccagcgag tggagcgttt tttcgttcgc aacaggctgt acaatccagg
     6121 agtaatcacc acgtgaaccg ggaataatca ccagaccgtt gtcatccggc gtggcacctt
     6181 tacgatgcaa ccagccctgt tgatcgccga tttgacacgc gctaacaaag ttatgcgcca
     6241 catccagaac cggacttccc gtggccttaa cttgttgcat tatgcgcaaa gcgatcatct
     6301 ggcgattaat acacgcaaac gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt
     6361 cgtcactgcc ttgaggcaaa ccatgatgcg agaacgaggc aatatgccgc tgtaaaatag
     6421 actgccccac cccccgcgag ccgctatgaa ccaacagttg cagatgttgt gcatccagac
     6481 ctgccagtgc aaacagttca gcgttgatga tttgatcaac ttgttgcagt tcagcgaaat
     6541 gattgccgcc accgatagag ccaagcgagc tgcgccacgg atgctgtgca aatgctgacg
     6601 gcaggttttc ctccagccag ctttcttcag caacgtcatc cagatctgat aatcgctttt
     6661 caaacttatc ggcgttgtat ttgcgagtga gaatatctgt ttgccatagc gccataccgc
     6721 agccgatatc attgccgacc agtgccggat aaaaacgacc aacagagaag aacgctgcgc
     6781 caatcggata gccgcgtccg ggatgtaaat ctggcatccc cactacgcgc tgcatgttgg
     6841 gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac aggtcatcag
     6901 atgcgatggt aaataccgca tcggataagg gacgaatata attgcccata taccattccg
     6961 taaatagact cgtcatactt caagttgcat gtgttgcgac ttgaattatt gagagtaaca
     7021 attaaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat taatcctgga
     7081 aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt gcagtttgtg
     7141 gaataagaaa acgcgcacat actaggcttg cgttattttc attgcaagct ggatttaatg
     7201 ttgctgttta taacgcatat aaaaattaat aaaccactct ccccgacatt caacgcgttg
     7261 ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta tgcttgcgtc
     7321 caccgtaagt tcggattcag acttaatcaa aggttttaac aaagcgctgg caaccccacg
     7381 gcgggtgtat tcagggtcaa caaataacat atcgatatag tgttcaacgc aggtaataaa
     7441 gccgaccagt tttgcattaa tgactgcaac ccgcacttgt gatttcgcga gtttctcctt
     7501 ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat aatgctgact
     7561 ggcagtcatc gtaaccgctc taatgaaaat agcgcatagt tgctgaaaat cgccaggctg
     7621 atagtctctt atttgtatgt tattcataat cccagcacca gttgtctttc catttgcggg
     7681 tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg gcgttcatcc
     7741 caggttttac gcgcggtggc gattaagtca gctttattca gccgcggcca gacgtgctcc
     7801 tgagtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgggc aatcagtaaa
     7861 tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc gataatcgct
     7921 tcacattcag accagtggtg gatatcttcc gccatcgtgc gttccacgcc gacggatttt
     7981 cgcaaccgtt cgctgttaac gtcgcgctcg tcaatcccct gactacgctc ccacaaaatg
     8041 cggccaaatt tgccaaagcg tttaagcagt atcaccagat cacacttttg tacatcaccg
     8101 caggtccgca gccccatcgc ttccagtttt gccgccgaga ctttgccgac gccggggatt
     8161 tttgccagcg gtaaggtttg taaaaatgcc ggaacttctg ccggcgtaat cacaaactga
     8221 ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg tgccacgccc
     8281 gcagacgctg tcagttgcag ctcgctgaaa atcgtctggc ggatttcctg ggcgatgagg
     8341 gtcgcagaac cgtggcaatg gacgctatcg gtgacgtcga gataagcctc atccagtgac
     8401 aatggttcaa tacgcgaggt gtagcgcgag aagatttcac ggatatgatt tgaggcttct
     8461 ttgtaggcgt caaagcgccc cggaagcaag gtgagatgcg ggcataattt gagcgccatc
     8521 cctgtcggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc ggtgctgatc
     8581 accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag ggcgggattg
     8641 tcgcgcatct ccaccgctgc gaaaaagcag tccatatcca catgaatgat tttacgcatt
     8701 actcacctct caacactggt aaagtataca gtgatttcag ggtttgagaa atgcgtaaag
     8761 attcagcatt taacgcgttc gctgcgaaag cacctgctgc ttatccagct tttgcaccag
     8821 cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat cggacgcact
     8881 tttggtcagc accataatct caatgcgccg gttgcccgcg ctttgcggat ttttggaatc
     8941 cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg gcattccggc
     9001 ctcttccagc acccgacgag ccgaaagcgc gcggtcaccc gaaaggttcc agttgttgta
     9061 gatattgttt ttgtaagcca tcgcatcggt atgcccggta ataataattt tgttatcgag
     9121 cgagtcgaaa actggcgcga gctccaccag cagcgtttta aagaacggca taatctgcgc
     9181 gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta atcagcacgc gtaatccctg
     9241 gggaacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa tggtggtgat
     9301 actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg ccgttttctc
     9361 attcacatca cgcgcttttt tctccgtttc ttccggcacc gccacggtgg caggttttgg
     9421 cggatgagac gggctgattt tatttaacgg cgacagccca ccaccgttaa aaatcgactg
     9481 cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt tgacaatcca
     9541 cagggtcata aacagcgcca tcattgccaa agtaaagtcg gcgaacgcca ccttccacgc
     9601 cccgccatga tgcccggcat gattcttttt gatctgccgc cggacgatgg tggtttttgc
     9661 cccacgctcg cggcggttgc ccgtctttct catgcttatt cttgctccag catcgcattc
     9721 acccacgcgt cgaggttggc gaaggtcggt ttggacgcca gatgcagcag cttacgcccg
     9781 gcatccaccg ccagcagcgt cggtttgccg cctgcctgcg ccaccagcac agtgcgcacg
     9841 cattccagca aagaatgttc tgcccgcgcc tgttgttcca tggcgttggc gagcgggtcc
     9901 atcaggcagt agcagataaa gacgcctaaa aacgtcccca ccagcgctgc cgccactttc
     9961 aggccgatca gggcaataga gccgtcaatc gactgcatgg tgatgatgat ccccagcact
    10021 gctgcacaaa taccaaatcc cggcatcgct tcggcggtac gttgcagcga gcgcgacggc
    10081 gtgagcagcg actcttccgc agtgtccagt tcctgatcga gaatgccttc cagttcatgg
    10141 gcgtcgattt tacccatcgc catcaggcga aagttatcgg caataaaggt caccagccgc
    10201 ttctgggtta acaccagcgg atatttctgg aaaatggtgc tctcttccgg ttgctcaata
    10261 tgttgatcca gcatgcgcag accaccgttt tgcaccattt cgagcaactc atacaggcac
    10321 attaatagct gacgctgaaa ctccggcccg agctgttttt tactgatcac gcctttaatt
    10381 tgatgggcaa tctctttcag cacatgtttc gggttaccaa taatcatcgc cccgaaaccg
    10441 gcacccaaaa taatgatcat ttccgccggt tgccagatag ccactaattg cccgcccgct
    10501 tcaaaataac cgccaaatac gcagccgagc accaccagta aacctaatat tttttgcatg
    10561 atttcttcac tttaattcgg atagacaaaa tcacgaattt tctgcgcgat ctgtttattc
    10621 agttgacaaa ttcgggcctc ggttagccct aataccagcg caatctcttt gagattcatc
    10681 tcatgctggt aatacatgga gaggatcagt tgctcttttt cgcttaattg tgaaagcgcc
    10741 tgttgcaaca tttgctgggt gacaaactga tcttccagct ccctgccagc aagcagaatg
    10801 ccaggccctt cgccgttcag cagttcgtcc aggctggcga gcgtttccgc cccttccagt
    10861 tgctgatatt cgagataatc ttcatgcgag atcccttcca cggccagttc tgaccactcc
    10921 ggttcgtgcc ccagtttttt gcgcgtttcg cgaatcagat ctttcatctg atgatatttc
    10981 tgtcgcagct ggcgcggtcg ccagtcgaga gcgcgcagct catccaggat cgcgccgcga
    11041 atacggtgca cagcatagcc cgcaaaacct tcgtccggca gcccgtagcg gcggatggca
    11101 ttaagcagcc ccattaacgc cgtttgttcc atatcctgac gatcaataat gcaattacac
    11161 tgcggggcaa gttgccgcac cactttacgc accaacggta aataggcctg caagtagtgg
    11221 ctctcctgct gcggggttaa taccggcgcg gcagcaaatt cttctgactc gaatacatcc
    11281 tgtagcatgt ttatatccgc ctggtgatta ctggaagacc acttttttca ccagcaaatc
    11341 cttatacggt gcgctcattt tgcgacgctg aagatctttc tttaacgcgt cagaaatgag
    11401 tttgcggatg gcggatattt tcatgccacg caaatcttca aatttcattt ccgtcaggca
    11461 ttcaaccgca atactttgat acagcggctc ctgatgtttt attttttccg cttctttgtc
    11521 actggcgacc accatcacta attccgccga caaataatgg tccgcgccgt tattgtcatg
    11581 cagcgtgaca atggtttccg gcaacgtcac aaacacgctt ttgctctcat ccagggtttc
    11641 caccgtttcc gtctgcgcgg cggcagtcgg ttttcctttt ccggcataat gatgccagac
    11701 accccatcca gctccggccc ccaccaccaa cgccagaacg gcgctgacca cgcccgccac
    11761 cacgattttc ttcatattgc ctttacactt tgatcaaaac ggattcgttc tgttgaaatt
    11821 gaggcgcatc atcaagctgt aaggcagcac tcacctcttc ctgctgatgg cccgactgtt
    11881 gttgttgctg cgactgccct tcggaggaaa cctgtacgtt cacctccata aaattttgcg
    11941 ccgtcagatg ctggcggaga tcgtcgctaa actgctgtaa cgcgcggcaa acttcacttt
    12001 ggttcgcgcc gatgtgcacc atcagtttgc cgttgtcgag ttgtacggcg atctcgagct
    12061 tgccaagcga cggtggatcg agacggatgg tggagatctg ctgttgtttg ttgagctgaa
    12121 agtggatctg atctttcaac agcgtcgtca gtttttcgcc cagttcatcc atcgaaagcg
    12181 tggctggttt ggcgacaatc tcctgatgat cgctgaccag ctccggcgtc ttaacctgca
    12241 acaccgcacg cgcaataact ggttcgctgt ccggcacttt ttccgtttta cgctcgacct
    12301 gcgggcgcgc ggtcacacgg gttagttcag gtttcgggcg agctggctgt gttgagacgc
    12361 gcggctgcgt cggcgcaatg gcatgtaaat cttcactggc gtaagtcgct aatctggcct
    12421 gctgttcggg cttcgcctgc ggcaactgac tgatcagttc ctgcaactgc ggcggcaacg
    12481 gcgtgagttc ctgctgttgc gtcgggcgtt gcggcgcgtt ttgcaccacc gctttcgtca
    12541 attgctggat aaccggcgat gttgcgacgt ttcgcggctg cggcgtgtcc tgatgcggcg
    12601 cggcaggttg cggaagtaac agcgccatta gcgcctgcat cgccaccgga tcgttctcct
    12661 gcgggcgcgt cgcttgttgc tgactggttt tagcgctatg aacgcgctcc gccaccgccg
    12721 cgaccgccat tttcggcagg gtaaacgcgg gcgcgttttc accgctcacc ggcggtaaaa
    12781 tatccgcctt caacgaagcg gtttccgcaa gcgttccgag cgtggcaagc aatgccggat
    12841 tcatgacttc tccgaaagta aattaacaat tgagtaggcg agcacacctt cctgatgccg
    12901 ctgatgctgt tccatacgcg ccttcaacac ttctgcgcgc tgctcgcgcc gtttaatcag
    12961 ggcattaaac gcgccgtaca gtcgcgcctt caccgcacac aactgcgccg cgtcggtttc
    13021 cgccgccgcg taatcgtgaa actggcgtat caactgctga tgcagtgctg gaaggcgtcg
    13081 ccagcgttgc ttgtcgagag cttcattcat cgcctcgacc agtgcgaaaa gttgcctgcg
    13141 ttgtttttcc atcgtcttaa cccagcttgc ccgaaagccc ttcccagcct tcgcgcaggt
    13201 tgcttaagat cagcatcact tcatcgattt tctctgccga cagttcgccg ctggcctcat
    13261 acaatcggta aacgcagtgg tcatagagtc gcgaaaggtt gaccaccagc tcgccgccag
    13321 tttcaaactc cagcgaactg gtgagcgcat tgagaatgtc gatgcattta ttgatgctct
    13381 gggctttctt ctcgaaacgc cgcccttcaa tatggctttt ggcgcgttcc agttcgtcca
    13441 tcaggccaga aaacagcacc agcaccagtt ccagaggcga tgccgccgca gtacgcgccg
    13501 cgagatcgat ctctttatat tgcgaatagc gcccttctgc tttcggcatt gccttccaca
    13561 atatagctgg cctgtttttt cagatgggtt tggtactgtt ttaataaacg cgcacgattt
    13621 tgcatccgct ggcgggcggc gtagctgaaa gtgatatgtg atgtggtggc gctggcaaat
    13681 gaagaaaacg gcaagcaaag caacagtaat cccgacaaca caaaagaggg aatcgacggc
    13741 aaggacatac tcaataatgg ctgatcatgc attgatgttg cagtgtatcc cggcc
//
LOCUS       DAPHDX010000118        13794 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPHDX010000118 DAPHDX010000000
VERSION     DAPHDX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.119"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            240..2141
                     /locus_tag="RH058_003482"
     CDS             240..2141
                     /locus_tag="RH058_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV3139021.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            2169..2480
                     /locus_tag="RH058_003483"
                     /pseudo
     CDS             2169..2480
                     /locus_tag="RH058_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2474..3370
                     /locus_tag="RH058_003484"
                     /pseudo
     CDS             2474..3370
                     /locus_tag="RH058_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            3639..5876
                     /gene="nrfB"
                     /locus_tag="RH058_003485"
     CDS             3639..5876
                     /gene="nrfB"
                     /locus_tag="RH058_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV3139022.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            5863..8835
                     /gene="nfrA"
                     /locus_tag="RH058_003486"
     CDS             5863..8835
                     /gene="nfrA"
                     /locus_tag="RH058_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDV3139023.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            8836..9726
                     /locus_tag="RH058_003487"
     CDS             8836..9726
                     /locus_tag="RH058_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV3139024.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            9909..10657
                     /gene="envY"
                     /locus_tag="RH058_003488"
                     /pseudo
     CDS             9909..10657
                     /gene="envY"
                     /locus_tag="RH058_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
     gene            11170..12123
                     /gene="ompT"
                     /locus_tag="RH058_003489"
     CDS             11170..12123
                     /gene="ompT"
                     /locus_tag="RH058_003489"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDV3139025.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            12310..13794
                     /locus_tag="RH058_003490"
     CDS             12310..13794
                     /locus_tag="RH058_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3139026.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         3699 a         3413 c         3443 g         3239 t
ORIGIN      
        1 ttgttactcg ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca agaggttgag
       61 cttgtgatta tttttttgac tgaacagaca cccgtttctg taaagtaata tttaaacaaa
      121 attctcacat aaccatagca ttttctgttc cttccctaat aacgtctgaa gtcgtcccgc
      181 ttatcacatt atccgcagtt gtacggtacg gatcattatt cattttactg gaggttgcta
      241 tgtcaaccgg attacgtttc acactggaag tggacggcct gccgccggat gcttttgcgg
      301 tggtttcctt tcatctgaac cagtcactct cttcgctctt ttccctcgat ctctccctgg
      361 tcagccagca gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga
      421 cgatatggca gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc tggtttgaac
      481 tcggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg ccgctgtggc
      541 gtgccggtct gcgccagaac ttccgtatct tccagaacga ggacatcaaa agcatcctcg
      601 gcacgatgtt gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc gagccgcatc
      661 cttcccgtga gttttgtgtc cagtacggtg agactgatta cgatttcctg tgccggatgg
      721 cggcggagga aggcatcttc ttttatgagg agcatgctta caaaagtacc gaccagagcc
      781 tggtgctgtg cgacacagtc cgccatctgc ccgaatcttt tgaaatccca tggaacccga
      841 acacccgtac cgaggtgagc accctctgca tcagccagtt ccgctacagc gcacaaatcc
      901 gcccttcttc cgtggtgacc aaggactaca cctttaaacg acccggctgg ccagggcgtt
      961 ttgatcagga aggccagtac caggattacc agcgcacgca gtatgaagtg tatgactacc
     1021 ccggacgttt caagggtgcc cacgggcaga actttgcccg ctggcagatg gacggctggc
     1081 gaaacaacgc agaagtggcg cgcggaacaa gccgttcgcc ggagatatgg ccgggacggc
     1141 gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcaa
     1201 gtgaactgca cggcgaacag ccacaggcgg tgccgggacg cagtggttca ggcaccacgc
     1261 tgaataatca ctttgcggtg atcccggcag acagaacatg gcgaccacag ccgttgctga
     1321 aaccgctggt ggacggcccg cagagcgctg tcgtgacagg accggcaggc gaggaaatct
     1381 tctgcgacga acatggtcgc gtgcgggtga agttcaactg ggaccgttat aacccggcag
     1441 accaagacag ttcgtgctgg atccgtgtgg cacaggcgtg ggcaggcacc ggttttggcc
     1501 acctggcgat accgcgtgtg ggtcaggagg tgattgtaga cttcctcaac ggcgatccgg
     1561 accagccgat cattatgggg cgcacctacc accaggaaaa ccgcaccccc ggcagcctgc
     1621 cgggaacaaa gacgcagatg accatccgct ccaaaacgta catgggcagc ggatttaatg
     1681 agctgaagtt tgatgatgcg acagggagag aacaggtcta catccacgcg cagaagaaca
     1741 tggataccga agtgctcaac gaccgtacca ccaccgtaaa acacgatcac cgcgaaaccg
     1801 taaaaaatga ccagacggtc acgatccagg aaggtaaccg ccttcttacg gtagaaaaag
     1861 gccacaagat caccggagta ctgaaagggt ctttatctga ggatgtcttt caggacagag
     1921 gcacgattgc cggttcggtg catgttgacg ctgtaaacaa tggtggcgaa ggcgacggta
     1981 tacaggctta tacggcgatt aaggaaattt tgctggccgt ggaggaaagc aaaattgcgc
     2041 tgacgccgga tggcattcag ctacaggtcg gggaatcgac ggtaatcagg ctgtcgaagg
     2101 atggcatcac catcgtgggc ggttctgttt tcatcaactg attcactcag attatcaggg
     2161 agtattttat gcagttcact tttaatgaag gccacattca actgccgtct caatggcagg
     2221 atcagtcaat gcaggtcctg gtgtccacag ataacagtgg catcaacctg gtcattaccc
     2281 gcgaagccgt tccacaggga accctgacac cagagctgta ccaggaaacg ctggcgctgt
     2341 accagggcaa acttgatggg tacaccgaac atgcctgccg ggaaatcact ctggcagagg
     2401 ccccggcctg gctgctggat tattcctgga acggtccgga agatgagggg aatcaggggc
     2461 ggattagcca gatatcagtc ctgcaaaatt tcacgagtgc tttattaact ggatgcgtga
     2521 ttgccattca tcaaatgata aagacgtcat tgcaattgat ggaaaaacgc tccggcactc
     2581 ttatgacaag agtcgccgca ggggagcgat tcatgtcatt agtgcgttct caacaatgca
     2641 cagtctggtc atcggacaga tcaagacgga tgagaaatct aatgagatta cagctatccc
     2701 agaacttctt aacatgctgg atattaaagg aaaaatcatc acaactgatg cgatgggttg
     2761 ccagaaagat attgcagaga agatacaaaa acagggaggt gattatttat tcgcggtaaa
     2821 aggaaaccag gggcggctaa ataaagcctt tgaggaaaaa tttccgctga aagaattaaa
     2881 taatccagag catgacagtt acgcaatcag tgaaaagagt cacggcagag aagaaatccg
     2941 tcttcatatt gtttgcgatg tccctgatga acttattgat tttacgtttg aatggaaagg
     3001 tctgaagaaa ttatgcgtgg cagtctcctt tcggtcaata atagcagaac aaaagaaaga
     3061 gcccgaaatg acggtcagat attatatcag ttctgctgat ttaaccgcag agaagttcgc
     3121 cacagcaatc cgaaaccact ggcacgtgga gaataagctg cactggcgtc tggacgtggt
     3181 aatgaatgaa gacgactgca aaataagaag aggaaatgca gcagaattat tttcagggat
     3241 acggcacatc gctattaata ttttgacgaa tgataaggta ttcaaggcag ggttaagacg
     3301 taagatgcga aaagcagcca tggacagaaa ctatctggcg tcagtccttg cggggagcgg
     3361 gctttcgtaa tcttgccctg cctgctcacc tcctccaggc acttccacta catgatgacc
     3421 tgatcggtgc caccactcct cttcactatg acgttaacct cccctccttg acaccacgct
     3481 tttgtctaca ttctacgcaa acggcaactt ttataaccag tgtaaaaata acgtgccgca
     3541 atagtcatac ctattaatgg taaaaagctg tcacaattca taaaaaacct taatatacgc
     3601 caccctaaac ataaccagcg ttaatgtaag gtttttgtgt ggactggctt cttgatgttt
     3661 ttgcaacctg gctctacggc ttaaaagtaa tcgcgataac gttagcggtc atcatgttca
     3721 tcagcgggct ggacgatttt tttattgatg tcgtctactg ggtacgccgc attaaacgca
     3781 agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat aaaccagatg
     3841 aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac gggcgtcatc ggcaatatgg
     3901 ccgagctggc ggcgaccacg ctcgactacg aaaactatca tatctttgtt ggcacctacc
     3961 ccaacgaccc cgatactcaa cgtgatgttg acgaagtgtg cgctcgcttt ccgaacgtgc
     4021 ataaggtagt ctgcgcgcgt cctggtccca ccagcaaagc cgactgtctg aacaacgtgc
     4081 tggacgccat cacccagttt gaacgtagcg ccaatttcgc ttttgctggt tttattctgc
     4141 atgacgccga agatgtgatt tcaccgatgg aattgcgtct gttcaactat ctggtcgagc
     4201 gtaaagatct gattcagatc ccggtgtatc cgttcgaacg cgaatggacg cacttcacca
     4261 gcatgactta cattgatgag ttttcagagc tgcatggcaa agatgttccg gtgcgtgaag
     4321 ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg tttcagccgc cgcgccgtga
     4381 ccgctctgtt agctgacggt gacggtattg ctttcgacgt gcagagtctg actgaagatt
     4441 acgacattgg tttccgcctg aaagagaaag gtatgacgga aatttttgtc cgttttccgg
     4501 tggtggacga agccaaagaa cgcgagcagc gcaaattttt acagcacgca cggacgtcaa
     4561 acatgatctg cgtgcgcgaa tatttccccg ataccttttc gactgcagtt cgacaaaaat
     4621 ctcgctggat catcggcatt gttttccagg gctttaaaac ccacaaatgg acctccagcc
     4681 tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc aatcagtaac tttgtcagct
     4741 tcctcgcgat gctggtgatg ctccagcttt tgctgttgct ggcgtatgaa agtttgtggc
     4801 ccaatgcctg gcatttcctt tctattttta gcggcagcgc atggttaatg accctgctgt
     4861 ggctaaactt tggcttgatg gttaaccgca tcgtgcagcg ggtgattttc gtcactggct
     4921 actacggtct gacgcagggg ctgctatctg tcctgcgtct tttctggggc aacctgatta
     4981 acttcatggc caactggcgc gcgctaaaac aggtacttca acacggcgat ccacgtcgcg
     5041 tcgcgtggga taaaacaacg catgacttcc ccagcgtgac tggcgatacc cgctcgttgc
     5101 gcccgttagg ccaaattctg ctggaaaatc aggtcatcac tgaagaacaa ctcgatacag
     5161 cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg caggggctga
     5221 ttagcgccga gcagctggca caggcgctgg cagagcaaaa cggcgtggcg tgggaatcca
     5281 tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat gccagcctcc gtagcgctgc
     5341 attatgcagt actgccgctg cgtctggaaa atgatgagtt aattgtcggc agtgaagatg
     5401 gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa gtgcgttacg
     5461 tcattgttct gcggggacaa attgtcacgg ggttacgtca ctggtatgca cgccgacgcg
     5521 gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca tcagtggctc acggaacagc
     5581 agaccggtga aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc gaaatactga
     5641 ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc catgaacgca
     5701 gttctctgcc gctcggcaag tttttggtca ccgaaggcgt tatcagccag gaaacgttgg
     5761 atcgcgtcct gacaattcaa cgcgaattac aagtttcgat gcaatcacta ttactcaaag
     5821 caggtttaaa cacagaacag gttgcgcaac tggagtccga aaatgaagga gaataacctt
     5881 aatcgcgtca tcggatggtc tggtttactg ctgacgtctt tattgagtac cagcgcactc
     5941 gcagacaata tcggcaccag cgcagaagag ctggggctga gcgattatcg ccattttgtt
     6001 atttatcccc gtctcgataa ggcgctgaag gcacagaaaa ataacgacga agcaaccgcc
     6061 atccgcgaat ttgaatatat acaccagcag gtgccggata atattccgct gactttatac
     6121 cttgcggaag cctatcgcca ttttggtcat gatgaccggg cgcggctgtt gcttgaggat
     6181 caactgaaac gtcacccagg agatgcccga cttgagcgca gtctggcggc tattccggtt
     6241 gaagtgaaaa gcgttacgac tgttgaagaa ctgcttgccc agcaaaaagc gtgcgacgct
     6301 gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct ggcacaatta
     6361 cctgtcgcca gagcgcaact gaacgatgcg acgtttgctg catcgccaga aggaaaaacg
     6421 ctgcgaaccg atctgctgca acgggcaatc tacctgaaac aatggtccca ggcagatacg
     6481 ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg tcagtggttt
     6541 gacgtgcttc ttgccgggca gctggacgat cggatcctgg cactgcaatc acaggggatc
     6601 ttcaccgatc ctcagtcata tattacttac gcgaccgcgc tggcttatcg tggcgaaaaa
     6661 gcacgcctcc agcattatct caatgaaaat aagccgctgt ttaccacgga cgcacaagag
     6721 aaaagttggc tctatctgtt atctaaatac agcgccaacc ccgttcaggc attggcgaat
     6781 tatacggtgc agtttgccga taaccgccag tatgttgttg gcgcgacgct accggtgctg
     6841 ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc cgccaatgaa
     6901 atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta acaaggctga atctctgcgt
     6961 ctggcacgat tgctgtatca gcaagaaccg gcaaatctta cccgcctgga tcaactgacc
     7021 tggcaactga tgcagaacga gcagtcacgc gaagctgccg atttattgct gcaacgctat
     7081 cctttccagg gcgatgcgcg tgtcagccag actttaatgg cgcgactggc gtctctgctg
     7141 gaaagtcatc cttacctggc aacgccggcg aaggtggcga ttttatcgaa acccttaccg
     7201 ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa ttgcccggca
     7261 atagttcgct tgctgggcga tatgtcgcct tcctacgatg ctgccgcctg gaaccgtctg
     7321 gcaaagtgtt atcgggacac gctacccggt gtggcgctgt atgcatggct tcaggccgaa
     7381 caacgacaac cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta tcaggttgag
     7441 gactacgcca ccgcactggc ggcctggcag aaaatcagtc ttcacgacat gagcaatgag
     7501 gatctgcttg ctgctgccaa taccgcccag gcggcaggaa atggtgcagc tcgcgatcgc
     7561 tggctacaac aggcagaaca acgtggactg ggaaacaatg ccctctactg gtggctgcat
     7621 gcgcaacgtt acattcctgg tcagccggaa ctcgcactga gcgatctcac gcgctcaatc
     7681 aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga caatttatcg ccaacgtcat
     7741 aatgtcccgg cggcggtgag tgatttgcgc gccgcgctgg aactggaacc gaataatagc
     7801 aacatccagg cagcgcttgg ttacgccttg tgggatagcg gtgatatcgc acagtcgcgg
     7861 gaaatgctcg aacaggcgca taaagggctt ccggacgatc cggcactgat ccgacaactg
     7921 gcctatgtga accagcgtct ggatgacatg cctgcgacgc agcattacgc ccggctggtg
     7981 attgatgaca ttgataatca ggcgctgata accccactga ccccagagca aaatcagcaa
     8041 cgcttcaatt tccgccgttt gcatgaggag gtcggtcgcc gctggacgtt cagtttcgat
     8101 tcttctatcg gcttgcgttc cggggcaatg agcaccgcta acaataatgt cggcggcgca
     8161 gcgccaggga aaagctatcg tagctacggg caactggaag ccgagtaccg catcggacgc
     8221 aatatgctgc tggaaggcga cctgctctca gtttatagcc gcgtctttgc cgataccgga
     8281 gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct gcgctggaag
     8341 ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc agttgccgct gaacgggcaa
     8401 aatggcgcat ccgataccat gctgcgcgcc agcgcctcat tctttaatgg cggcaaatac
     8461 agcgacgaat ggcacccgaa cggttcaggc tggtttgccc aaaacctgta cctcgatgcg
     8521 gcgcaatata tccgccagga tattcaggcg tggacggcag attatcgcgt cagctggcat
     8581 cagaaggtag ctaacggaca gactattgag ccttacgctc acgttcagga caacggctat
     8641 cgtgataaag gcactcaggg cgcgcagctt ggcggagtcg gggtccgctg gaatatctgg
     8701 accggcgaga cgcactacga cgcctggccg cacaaagtca gtctcggcgt cgagtatcaa
     8761 cataccttta aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt tctcaccatt
     8821 ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc tgacactgct tttggtcagc
     8881 cctttttcct ttgcgatgaa aggtattatc tggcaaccac aaaaccgaga tagtcaggtt
     8941 accgataccc agtggcaggg gctgatgagt cagttacgtt tgcaaggctt cgataccctg
     9001 gttttgcaat ggacccgtta cggcgatgca ttcacccagc cagaacagcg cgctttattg
     9061 tttaagcgtg ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg gctgaacgcc
     9121 gatccggaat tttttatgca ccagaaacag tcgtccgcag cgctggaaag ctatcttaat
     9181 cgcctgctgg ctgccgatct ccagcaagcc agattatgga gcgccgcgcc tggcgtaacg
     9241 ccggatggct ggtacatcag cgcggaaatt gacgacctga actggcgcag cgaagctgcc
     9301 cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc tgattagcga tgtttcagca
     9361 aaaccggttt atatcagtag ttttttcgcc ggaaacatgt cgcccgatgg ctatcgccaa
     9421 ctgctggaac aagttaaagc aactggcgtt aatgtctggg tacaggatgg cagcggcgtg
     9481 gataaactta ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga ttgccaaagt
     9541 cccgcccctg ccagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg caaaaccttt
     9601 acagcgaaac cgaaaccgga tgcagagatc gcctcgctgt tagcgaaacg ttcctcttgc
     9661 ggtaaagaca ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg cattctcgag
     9721 tattaaatct cctccaggta agtcgggtac gacctggctt acctctttcg ctctcgtcag
     9781 aattccatca agattaattg ctaaaaagcg tgctaaagaa agaagatcgt tatcggcgaa
     9841 cgggccgatc actcatgcag gaagcataga tcctttgagc ggagtttctg atatctgagg
     9901 agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt gatcctgacc gaaaaagagg
     9961 tagaggtaag cgtcaataac catgctacgt ttacccttcc gaaaaactac ctggccgcct
    10021 tcgcgtgcaa caataacgtc attgaactct caacgttaaa tcacgtatta atcccccaca
    10081 tcaaccgtaa catcatcaac gattatctgt tgtttttacc tgtgtaaagc cctggtcgcg
    10141 gctggcaacc ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccgc
    10201 caaccacagc aagcagcaac ccagcaaacc ctgcgaagcg gagttgacac gcgcattact
    10261 ttttaccgta ttgtctaact ttcttgagca atcgcggttt atagccctac tgatgtatat
    10321 cttacgcagc agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta
    10381 ctggaatctg cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa
    10441 attaaaaaac gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc
    10501 cgtacagatg ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta
    10561 tagcagcacg tcatacttta tctctgtttt taaggcgttt tacggcctga caccgttgaa
    10621 ttatctcgcc aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag
    10681 acgggcataa atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac
    10741 ttagaagttc ctagaacgac attttaagtc aacaaattac cgcgccatct ctgcgctcac
    10801 acgtcccact acctcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc ttatgtgtct
    10861 cagttttgtc cctctttttt gtactaaaaa acatagcaat tgaggataaa cctcatgcta
    10921 ttttcgctta tatgccttta aaggcatggc gcttaaatag ataaaaacac cacaaaagca
    10981 taaaaaacca cacagtaaaa ccgcaacata aaacaataac agataattaa accaaaaaca
    11041 gatagcgcat tgtgataatc attcaatact aaacaaaata taaacagtgg agcaatatgt
    11101 gattgactca ttaagttaga tataaaaaat acatattcaa tcattaaaac gattgaatgg
    11161 agaactttta tgcgggcgaa acttctggga atagttctga caacccctat tgcgatcagc
    11221 tcttttgctt ctaccgagac tatatcgttt actcctgaca acataaatgc ggacattagt
    11281 cttggaactc tgagcggaaa aacaaaagag cgcgtttatc tagctgaaga aggaggccga
    11341 aaagtcagtc aactcgactg gaaattcaat aacgctgcaa ttattaaagg tgcaattaat
    11401 tgggatttga tgccccagat atctattggg gctgcgggct ggactactct cggcagccga
    11461 ggtggcaata tggtcgatca ggactggatg gattccagta accccggaac ctggacagat
    11521 gaaagtaggc acccggatac acaactcaat tatgccaacg aatttgatct gaatatcaaa
    11581 ggctggctcc tcaacgaacc caattaccgc ctgggactca tggccggata tcaggaaagc
    11641 cgttatagct ttacagccag aggtggttcc tatatctaca gttctgagga gggattcaga
    11701 gatgatatcg gctccttccc gaatggagaa agagcaatcg gctacaaaca acgttttaaa
    11761 atgccctaca ttggcttgac tggaagttat cgttatgaag attttgagct aggtggcaca
    11821 tttaaataca gcggctgggt ggaagcatct gataacgatg agcactatga cccaggaaaa
    11881 agaatcactt atcgcagtaa agtcaaagac caaaattact attctgttgc agtcaatgca
    11941 ggttattacg taacacctaa cgcaaaggtt tatgttgaag gcgcatggaa tcgggttacg
    12001 aataaaaaag gtaatacttc actttatgat cacaatgata acacttcaga ctacagcaaa
    12061 aatggtgcag gcatagaaaa ctataacttc atcactactg ctggtcttaa gtacaccttt
    12121 taacgaagtt aaccagattt tctccccggt ggtattgtat aaccccgggg aatttgttta
    12181 cagttacaag taggcaatat ccactaattt tgtaagcatt taacctatgt cataacgtgt
    12241 aatctcttac cgcaacacaa gacctcaata tattcaaatc ttatctttat tccgatattc
    12301 agagacacaa tgcgaaaaat aattactcat ttcaaagttg ttttaacgtt acttctacca
    12361 gtaaccgtat ctgcccagca gatacagtgg caatcctgta tggccagtca attcaaccac
    12421 tggtttggtg aggaaaaacc gtctcctgac ttactatgtg gttatttgtc tgttccatta
    12481 aaatatacag acacaggcgg agatgcttct tatgaaaaaa aatcacaagt caaactagcg
    12541 ttgacaaaat tgccggcaaa aagcaagcat aaaggaagta tcctgataat aagtggtggt
    12601 cccgggttac caggcataaa tccttatatt aactttgact ggccagtcac aaatcttcgt
    12661 gagtcatggg atattattgg atttgatcct cgaggcgtcg gacagtccac tccgacaata
    12721 aactgccggc aatcagatac agagactcag gaaaacataa ccgaaaagca acaagtatta
    12781 aataaaatta atgcctgtat ccataatacc ggagccgaag tcattcgcca tatcggctct
    12841 aacgaggctg tatacgatat tgatcgtatt aggcaagcct tgggggataa acaactgaca
    12901 gccgtggcgt attcgtatgg aactcaaatt gcagccttat atgcagaacg ttttccctac
    12961 aacgtaagat ctatcgttct tgatggagtc gtcgatatcg atgacctgga ggacaacttc
    13021 acatggcaac tcaaacaggc acagagttat caggaaacgt ttgatcgctt tgcatcctgg
    13081 tgtgcgcgta caaaaagttg cccgctttct tcagacagag ataaggcaat aactcagttc
    13141 catgagctat tatcaaaatt acatcacaaa cctttattag acagtaaggg agaaaatata
    13201 tcttcagatg aactcatatc attaacaaca gaccttctgc tatggcgttc atcatggcca
    13261 acccttgcaa ctgccatacg ccagttctct caggggattg tcagtaatga aattgaaact
    13321 gcgctcagtg ctccgatagc ctcagaagag tcaagcgatg cttcgggggt aatcctctgt
    13381 gtagatcagg gggatgagca attaacacca gaagagcgaa aatcccgaaa agacgctctt
    13441 gcgaatgcct tcccggctat taactttgac aatggacgtt ccgattcacc tgatttttgt
    13501 gaattatggc caatacatag cgacctgaac aaaactcgcc tgaaaaatac tgttctgccc
    13561 tctggtttac tgtttgtagc acacaaatac gacccaacaa cgccctggat taatgcccgt
    13621 aagatggcag agaaattttc cagcccgtta ctaacaataa atggtgatgg gcatacatta
    13681 gctctcaccg gagttaattt atgtgtagat aaagcagttg tacatcacct gatcactcca
    13741 caaaaaatag aaaatatata ctgtccagga aattctgaag cagaaataca ataa
//
LOCUS       DAPHDX010000119        13448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPHDX010000119 DAPHDX010000000
VERSION     DAPHDX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.120"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            162..860
                     /locus_tag="RH058_003491"
     CDS             162..860
                     /locus_tag="RH058_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3139027.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(905..1804)
                     /gene="eamA"
                     /locus_tag="RH058_003492"
     CDS             complement(905..1804)
                     /gene="eamA"
                     /locus_tag="RH058_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV3139028.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1999..3186
                     /gene="ydeE"
                     /locus_tag="RH058_003493"
     CDS             1999..3186
                     /gene="ydeE"
                     /locus_tag="RH058_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV3139029.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3313..3408
                     /gene="mgtS"
                     /locus_tag="RH058_003494"
     CDS             3313..3408
                     /gene="mgtS"
                     /locus_tag="RH058_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV3139030.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3627..4517)
                     /gene="dgcZ"
                     /locus_tag="RH058_003495"
     CDS             complement(3627..4517)
                     /gene="dgcZ"
                     /locus_tag="RH058_003495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV3139031.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4772..5164)
                     /gene="ydeI"
                     /locus_tag="RH058_003496"
     CDS             complement(4772..5164)
                     /gene="ydeI"
                     /locus_tag="RH058_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV3139032.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINAVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            5440..5958
                     /locus_tag="RH058_003497"
     CDS             5440..5958
                     /locus_tag="RH058_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV3139033.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(6003..8048)
                     /gene="dcp"
                     /locus_tag="RH058_003498"
     CDS             complement(6003..8048)
                     /gene="dcp"
                     /locus_tag="RH058_003498"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV3139034.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            8185..8931
                     /gene="ydfG"
                     /locus_tag="RH058_003499"
     CDS             8185..8931
                     /gene="ydfG"
                     /locus_tag="RH058_003499"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV3139035.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            9020..9706
                     /gene="ydfH"
                     /locus_tag="RH058_003500"
     CDS             9020..9706
                     /gene="ydfH"
                     /locus_tag="RH058_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV3139036.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            9884..10087
                     /gene="ydfZ"
                     /locus_tag="RH058_003501"
     CDS             9884..10087
                     /gene="ydfZ"
                     /locus_tag="RH058_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV3139037.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(10123..11583)
                     /locus_tag="RH058_003502"
     CDS             complement(10123..11583)
                     /locus_tag="RH058_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV3139038.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTETG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(11672..12955)
                     /gene="ydfJ"
                     /locus_tag="RH058_003503"
                     /pseudo
     CDS             complement(11672..12955)
                     /gene="ydfJ"
                     /locus_tag="RH058_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         3647 a         3325 c         2973 g         3503 t
ORIGIN      
        1 aaatatcata tcccgcagta gctatacgtc ctggtacatt gatcacagta atacagtgat
       61 aaagttgttc aacatcaggg tgattattaa acgactcggc catacgataa aacaatcagg
      121 actcaactcc ttgttttcaa tggctcttaa aaggtgcaaa agtgattttt caaaagattg
      181 cccgcttact taaatccgaa atcaatggca attcatggca tgttggtgat ttgttgccgt
      241 cagaagcgga actcgctgtt cgttataatg tttcgcgtaa taccctgcgt aaggcattat
      301 ccttgctgga aggcgaagga attattcaca gaaagcatgg ttcaggaaca tacattcaaa
      361 aaaagaattt tgttgcacac attgatcaca tgaacagttt cagtgaaatt gcgcataaaa
      421 gcggcaaaga ggcagggagt cagattatga aatttgaagt gcaggatgct tcacccacta
      481 ttgcgactga gctgaattta gtgactggtg agcaggttta ttacataaag agactgcgat
      541 tcattgagga taatgcagca caattagaag aaacgtggat gtcagtagca cgttttcctg
      601 atttaaccgt gtcacatatg caaaaatcca aattttcata tattgagaat gaatgcggga
      661 tcaaaatcat tggcaccttt gaaactttct cccctacttt tcctacccca gaaatcgcca
      721 gtattttacg gatcagccca cgggatccca tacttaaaat tcagacccag gctgtggaca
      781 gtaactctat tccgctggat tattcgttac tttacagcaa tattttcgag ttccaggtaa
      841 agtacttttt cccgcgataa aagcagacgc acagagtgct gacatcacat tctgtgcgta
      901 tggtttagcc tctaaccttt accgctttac gccaccgcaa gccaaataca ttgatataca
      961 gcccggtcat aatgagcacc gcacctaaaa attgcagacc cgttaagcgt tcatccaaca
     1021 atagtgccgc acttgccagt ccgactacgg gcaccagtaa cgataacggt gcaacccgcc
     1081 aggtttcata gcgtcccagt aacgtccccc agatcccata accaacaatt gtcgccacaa
     1141 acgccagata catcagcgac aagatggtgg tcatatcaat agtaaccaga ctgtgaatca
     1201 tggatgcgga accatcgaga atcagcgagg caacaaagaa gggaatgatt gggattaaag
     1261 cgctccagat taccagcgac atcaccgccg gacgcgttga gtgcgacatg atctttttat
     1321 tgaagatgtt gccacacgcc caactaaatg ctgcagccag ggtcaacata aagccgagca
     1381 tcgccacatg ctgaccgttc agactatctt cgattaacac cagtacgcca aaaatcgcta
     1441 aggcgatccc cgccaattgt ttgccatgca atcgctcccc gaaagtaaac gcgccaagca
     1501 cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca ggcataccga
     1561 agttaatggc acaaaaaaga aaagcaaact gcgcaaaact gatggttaat ccatacccca
     1621 gcagcaaatt cagtggtact ttcggtcgtg cgacaaaaaa gatagccgga aaagcgacca
     1681 gcataaagcg caaaccggcc agcatcagcg gtggcatgtt atgaagcccc actttgatga
     1741 ccacaaaatt tagcccccat acgaccacta ccagtagcgc caacacccca tcttttcgcg
     1801 acattctacc gcctctgaat ttcatctttt gtaagcaatc aacttagctg aatttacttt
     1861 tctttaacag ttgattcgtt agtcgccggt tacgacggca ttaatgcgca aataagtcgc
     1921 tatacttcgg atttttgcca tgctatttct ttacatctct aaaacaaaac ataacgaaac
     1981 gcactgccgg acagacaaat gaacttatcc ctacgacgct ctaccagcgc ccttcttgcc
     2041 tcgtcgttgt tattaaccat cggacgcggc gctacgctgc catttatgac catttacttg
     2101 agtcgccagt acagcctgag tgtcgatcta atcggttatg cgatgacaat tgcgctcact
     2161 attggcgtcg tttttagcct cggttttggt atcctggcgg ataagttcga caagaaacgc
     2221 tatatgttac tggcaattac cgccttcgcc agcggtttta ttgccattcc tttagtgaat
     2281 aacgtaacgc tggtggtgct cttttttgcc ctcattaact gcgcctattc tgtttttgct
     2341 accgtgctga aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc
     2401 tcaatcaact acaccatgct aaacattggc tggaccatcg gtccgccgct cggcacgctg
     2461 ttggtgatgc agagcatcaa tctgcccttc tggctggcag ctatctgttc cgcgtttccc
     2521 atgcttttca ttcaaatttg ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc
     2581 agtgtctggt cgccgaaagt tttattacaa gataaagcac tgttgtggtt tacctgctct
     2641 ggttttctgg cttcttttgt aagcggcgca tttgcttcat gcatttcaca atatgtgatg
     2701 gtgattgctg atggggattt tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct
     2761 gccatggtgg ttacgttgca atattccgtg ggccgccgac ttaacccggc taacatccgc
     2821 gcgctgatga cagcaggcac cctatgtttc gtcatcggtc tggtcggttt tattttttcc
     2881 ggcaacagcc tgctattgtg gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt
     2941 tatgcgccgg gcgagtatat gttgattgac catattgcgc cgccaggaat gaaagccagc
     3001 tatttttccg cccagtcttt aggctggctt ggtgccgcga ttaacccatt agtgagtggc
     3061 gtagtgctaa ccagcctgcc gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt
     3121 gctgcgtggg tgctgatgtt aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt
     3181 tgttgattta agtcgaacac aataaaaatt taattcagcc ttcgtttagg ttacctctgc
     3241 taatatcttt ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc acaccataaa
     3301 cggaggcaaa taatgctggg taatatgaat gtttttatgg ccgtactggg aataatttta
     3361 ttttctggtt ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata
     3421 atccctcacc taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg
     3481 gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg
     3541 cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag
     3601 ttttctcaat gaatgttaaa cggagcttaa actcggttaa tcacattttg ttcgtcaata
     3661 aacatgcagc gatttcttcc ggtttgctta ccctcataca ttgcccggtc tgctcttcca
     3721 atgaccacat ccagaggctc ttcaggaaat gcgcgactca cacctgctgt cacggtaatg
     3781 ttgatatgcc cttcagaatg tgtgatggca tggttatcga ctaactggca aattctgaca
     3841 cctgcacgac atgcttcttc atcattagcc gctttgacaa taatgataaa ttcttcgccc
     3901 ccgtagcgat aaaccgtttc gtaatcacgc gtccaactgg ctaagtaagt tgccagggtg
     3961 cgtaatacta catcgccgat taaatgcccg taggtatcat taaccaattt aaatcggtca
     4021 atatccaaca acattaaata aagattcaga ggctcagtgt tgcgtaactg atgatcaaag
     4081 gattcatcaa gaacccgacg ccccggcaat cccgtcaaaa catccatatt gctacggatc
     4141 gtcagcaaat aaattttgta atcggttaat gccgcagtaa aagaaagcaa cccctcctga
     4201 aaggcgtcga aatgcgcgtc ctgccagtga ttttcaacaa tagccagcat taattcccga
     4261 ccacagttat gcatatgttg atgggcagaa tccattagcc gaacgtaagg taattcatcg
     4321 ttatcgagtg gccccagatg atcaatccac cgaccaaact ggcacagtcc ataagaatgg
     4381 ttatccgtta tttctggctt actggcatct ctcgcgacca cgctgtgaaa catactcacc
     4441 agccactggt agtgggcatc gatagcctta ttgagattta acaagatggc atcaatttcc
     4501 gttgtcttct tgatcattgc cactcctttt tcacagttcc ttgtgcgcgc tattctaacg
     4561 agagaaaagc aaaattacgt caatattttc atagaaatcc gaagttagga gtcatctctg
     4621 agataacatt gtgatttaaa acaaaatcag cggataaaaa agtgtttaat tctgtaaatc
     4681 acctctgcat tatcgtaaat aaaaggatga caaatagcat aacccaatac cctaatggcc
     4741 cagtagttca ggccatcagg ctaatttatt tttatttctg caaatgagtg acccgaacga
     4801 cggccggcgc gcttttctta tccagactgc cgctaatgtt gatcatctgg tccggctgaa
     4861 cttctcgtcc atcaaagacg gccgcaggaa taacggcatt aatttcaccg ctcttatcgc
     4921 gaaaaacgta acggtcctct cctttgtgag aaatcaaatt accgcgtagt gaaaccgaag
     4981 cgccatcgtg catgtttttt gcgaaatcaa cggtcatttt ttttgcatca tcggttccgc
     5041 gatagccatc ttctattgca tgaggcggcg gtggcgctgc atcctgtttt aaaccgccct
     5101 ggtcatctgc caacgcataa ggcatgacaa gaaaacttgc taatacaatg gcctgaaatt
     5161 tcatactaac tccttaattg cgtttggttt gacttattaa gtctggttgc tatttttata
     5221 attgccaaat aagaatactg ccaattgtta taaggcattt aaaatcagcc cactagctgt
     5281 caaatattca gagaatttaa ctcactaaag ttaagaagat tgaagagtct taaacatatt
     5341 ttcagaataa tcggatttat atgtttgaaa attattatat ttgacgagca tacagaaaaa
     5401 gcaaatcacc tttacatata atagcgtgga caaaaaacag tgaacattaa taaagataaa
     5461 attgtacaac tcgcagatac cgacactatt gaaaacctga catccgcgtt gagtcaaaga
     5521 cttatcgcgg atcaattacg cttaactacc gccgaatcat gcaccggcgg taagttggct
     5581 agcgccctgt gtgcagctga agatacaccc aaattttacg gtgcaggctt tgttactttc
     5641 accgatcagg caaagatgaa aatcctcagc gtaagccagc aatctcttga acgatattct
     5701 gcggtgagtg agaaagtggc agcagaaatg gcaacaggtg ccatagagcg tgcggatgct
     5761 gatgtcagta ttgccattac cggctacggc ggaccggagg gcggtgaaga tggtacgcca
     5821 gcgggtaccg tctggttcgc gtggcatatt aaaggccaga cctacactgc ggttatgcat
     5881 tttgctggcg actgcgaaac ggtattagct ttagcggtga ggtttgccct cgtccaactg
     5941 ctgcaattac tgctataacc aggctggcct ggcgatatct ccaggccagc cattggtggt
     6001 gtttatatgt tcaagccacg atgttgcagc atcggcataa tttgaggagc cttaccgcgc
     6061 cattgtcgat acaggcgttc cagatcttcg ctgttacctc tggaaaggat cgcctcgcga
     6121 aaacgctgcc cattttcacg cgttaatccg ccctgctcaa caaaccactg gtaaccatca
     6181 tcggccaaca tttgcgtcca cagataagcg taataacctg cagcatatcc gccaccaaaa
     6241 atatgggcga aataactgct gcgatagcgt ggcggtatag caggaagatc catattttcc
     6301 gccaccagcg cccgcaattc aaaatcatcg acatcctgca ttgcttcgtt ttcttccagg
     6361 caatgccagc gcatatcgag aagtgcggcg ctaagcagtt cgctcatctc ataccctttg
     6421 ttgaacaggc tggcattacg cattttctgt tgcagttcgt caggcattgc tgccccgctc
     6481 tgataatgcc gggcgtagcg agcgaatacc tgcggatgcg ttgcccagtg ttcgttgatt
     6541 tgcgacggaa attcgacaaa atcacgcggc gtgttggtgc cggaaagcgt ggcataacgc
     6601 tggcgggcaa aaaggccgtg cagcgtatga ccaaattcat ggaataaggt tattacatca
     6661 tcccagagta acaacgcagg ctcaccggca gcgggtttct gataattgca aacgttataa
     6721 attaccggat gcgtttcatt aagcgttgat tgctcaacaa aattgcccat ccatgcaccg
     6781 ccgctttttg aatcacgggc gaagaaatca ccgtaaaata acgccagtcc cacgccatta
     6841 tgatcaaaaa tttcccacac acgaacgtca ggatggtaga caggaatatc aaaacgttcg
     6901 acaaacttaa taccgaagag ctgattcgcg gtccagaata caccttcatt caacaccgtg
     6961 tttaattcaa aatatggctt gagctgcgcc tcatcaagat caaatttctc ccgccgtacc
     7021 tgttcggcat aaaatgccca gtcccacggc tgcgcgctaa acccgccttg ctgcttatcg
     7081 ataaccgcct gtatggaggc taattcatcg ctagcacgtt gacgcgccgc tggaacaatt
     7141 tcccgcataa agttgagtgc tgcttcaggt gtttttgcca tctgatcggc gattttccat
     7201 gcggcataat gaggaaaacc aagcagtttc gcctgctgtg cgcgaatctc taccagacgt
     7261 tgaatgatag cgcgggtatc attgccatca tttttttccg ctcgcgtcca gcccgcaata
     7321 aacagttttt cacgcgtcgc acgatcgcgc atttcggcaa gcgccggttg ttgggtggta
     7381 ttcagcagcg gaatcagcca tttgttatcc agacctttct cgcgagccgc cactgccgcc
     7441 agcgcaatct cttgctcact cattcctgcc agctgcgcga tatcgttcac aaccagaccg
     7501 ccggatttat ttgctgcaag taaccgctgg ttaaactggc tggtcagggt cgcagcttct
     7561 gtattcagta cttttaattt tgctttatca gcttgcgcaa gtttggctcc ggcaaggaca
     7621 aaacgttgat gaataacctc caccaggcgg atggattcac tatcaagccc cagggattca
     7681 cggcgctgcc agacagcatc tacccgcgcg aataattcac cgttcagata gatatcatta
     7741 gccagttccg ccagttcagc ggaaaactgt tcatcaagac gctgtaattc atcattggta
     7801 tgcgccgcag tcatcgcaaa aaagacgctg gtaacgcggg taagtaattc tccgctttgt
     7861 tccagtgcca gaatagtatt gttgaaatca ggcgtttgcg ggttaagcgc gatggcagca
     7921 atttctgccc gcttttgctg cattccctca tcgaatgccg ggcgatagtg atgattggca
     7981 atttgatcaa aatgaggagc cagatacggc agtgtgcttt gcacaaggaa aggattcatt
     8041 gttgtcattt tcttctcctg aacgcgaggt gttccatagc gtaggcttac tgataacgga
     8101 gtgcaatctt gcaaaccagt attacccgct cttaagcatc acgtgctatg ttattgacac
     8161 acaaaagcgt tgaggaacag tgagatgatc gttttagtaa ctggagcaac ggcaggtttt
     8221 ggtgaatgca ttactcgtcg ttttattcaa caagggcata aagttatcgc cactggccgt
     8281 cgccaagagc ggttgcagga gttaaaagac gaactgggag ataatctgta tatcgcccaa
     8341 ctggacgttc gcaaccgcgc cgctattgaa gagatgctgg catcgctccc tgccgagtgg
     8401 tgcaatattg atatcctggt aaataatgcc ggcctggcgt tgggcatgga gcctgcgcat
     8461 aaagccagcg ttgaagactg ggaaacgatg attgatacca acaacaaagg cctggtatat
     8521 atgacgcgcg ccgtcttacc gggtatggtt gaacgtaatc atggtcatat tattaacatt
     8581 ggctcaacgg caggtagctg gccttatgcc ggtggtaacg tttacggtgc gacgaaagcg
     8641 tttgttcgtc agtttagcct gaatctgcgt acggacctgc atggtacggc ggtgcgcgtc
     8701 accgacatcg aaccgggtct ggtgggtggc accgagtttt ccaatgtccg ctttaaaggc
     8761 gatgacggta aagcggaaaa aacctatcaa aataccgttg cattgacgcc agaagatgtc
     8821 agcgaagccg tctggtgggt gtcaacgctg cctgctcacg tcaatatcaa taccctggaa
     8881 atgatgccgg ttacccaaag ctatgccgga ctgaatgtcc accgtcagta atttttatac
     8941 ccggcgtaac tgccgggtta ttgcttgtca caaaaaagtg gtagactcat gcagttaact
     9001 cactcacaag caagaacgaa tgaccgtcga aacgcaactt aatcccacac agcctgtcaa
     9061 tcagcagatt tatcgtattc ttcgtcgcga cattgtccat tgcctgattg caccaggcac
     9121 accgttgtcg gaaaaagaag tttctgttcg tttcaatgtg tcacgccagc cggttcgtga
     9181 agcctttatt aaactggcgg aaaacggcct gattcaaatt cgtccgcagc gtggcagcta
     9241 cgtcaacaaa atttccatgg cccaggtgcg aaacggcagt tttatccgtc aggccattga
     9301 gtgcgcggtg gcgcgtcggg cggcgagcat gattaccgaa agccagtgct accaactgga
     9361 acaaaatctt caccagcaac gcattgccat tgagcgcaag caactggatg atttttttga
     9421 acttgatgac aacttccatc aactcctgac gcagattgcc gactgtcaac tggcgtggga
     9481 taccattgag aacctgaaag cgaccgttga tcgcgtgcgc tatatgagtt tcgaccatgt
     9541 ttctccacca gaaatgctgt tacgccagca tcttgatatt ttctctgccc tgcaaaaacg
     9601 tgatggcgat gcggtagaac gtgcaatgac gcaacatttg caggaaatca gcgaatccgt
     9661 gcgccagatc cgccaggaaa acagcgactg gtttagcgaa gagtaattca tttactctca
     9721 tcccatccgg gatgagaggc ttttcccccc gccttacggc tcatgcatgc atcaaaaaag
     9781 atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta tattgcgccc
     9841 gttacgtggg cttcgactgt aaatcagaaa ggagaaaaca cctatgacga cctacgatcg
     9901 taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg gtcacactgg
     9961 caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc gcggaaaaac
    10021 cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc gtctcggcat
    10081 gaactaagtg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca cggaatattt
    10141 cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg tcacttttgc
    10201 agtaaacaag ctattgtctg gcaaatcatc accaaagatt gccttaatcg ccagcaatga
    10261 ctggacgcgc gctttccctt cggcactact ttgtacagcc ttctgaataa caggtaacag
    10321 tgggtcactg atttctatcg gatttccctg ttcatcaaca ccaccgacat aacgcatcca
    10381 acccgcgacg cccagcgcca gcagatcgaa cttgctgtca tgcgccagat gccagcgaac
    10441 agaatccaac atccgctgtg gcaatttctg gctaccatcc atcgcaatct gccaggttcg
    10501 atgacgtaac gccgggttgc tatagcgtgc aattaatcgg ttagcgtaat cttgcaaatc
    10561 aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat acgccgcata
    10621 acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa gataacccaa
    10681 atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt cataaggcag
    10741 cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg caacaaagtt
    10801 atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat cgcccacacc
    10861 ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa tacggtccac
    10921 cattgttgat gggaaagtca cgttatcttc gatccattgt gccagttttt catcaacggc
    10981 ttgcgcgtag gaagtgacaa cgtcacgcat aacatgaccg ttttctggca tgttgtcaca
    11041 tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgagccagcg cctcaacaat
    11101 cactcctgtt tctgttttcg gctggtgggg attttgcacg tcggcagcga ccatcgggtg
    11161 atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg tgattgtcag
    11221 agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta agccatccat
    11281 ctgtacgtgc aaggcttttt taacgacgcc aacgacgcga gccgtccacg catcggccga
    11341 catttccgca acggtataaa gattatcttg ctgctgtaaa tcggcaattt gctgttcgcc
    11401 gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag caagaatatc
    11461 ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa caattcttgg
    11521 ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca aattatttcc
    11581 cataacaatt ccttaaattt aaatatggca agctacatgt tttgttatat gaataaaaat
    11641 cccctctccg gtaagagaag ggattgagga tttacagact tctggaaggt tgcgcagctc
    11701 ttacaacacg cggttgatct tccgcagcgt cttccagcgc acttaaatca cggtctttca
    11761 cttctggcat tttcagcgca gagattaaac caatcactga atatgccatg atcataatgg
    11821 cgatcggata ccaggattcc gtcatggtgc agaaaatacc cgccaggata ggaccaaaac
    11881 cggaagcgat aagaccacca atttctttag aaatggccat ccgggtaaag cggtttttac
    11941 agccgaacat ttctgccatg gtaatgtttt ccagagcaaa taatcccagc accgcacagt
    12001 tatgaatcac aatcagtgca accataatgg tgctcggggc atagctttta tctacaatga
    12061 tagaaagcat tggccatgcc agcacaatcg cggaggtatt cataataata tacgggatcc
    12121 ggcgaccaat tttatcggat aaccaaccaa ggaacggaat ggtcataaag ccgagaatcg
    12181 aactaatcat caatgcatct gttggaattg ctttgttaaa caataacgtc tgcactaaat
    12241 agcctgcaag gaaagtctga attaacccgg agttacccgc ctgaccaaaa cgcagccctg
    12301 ttgccagcca gaaggatttg ctctggaaca tgctaccagc aggtgcaggt tttgctgtgg
    12361 gttggttgct gtcattaacc ttctcaaaga ccgggctttc tttcagattc atacgtaacc
    12421 agatagcaaa gaccatcacg acaacgctcg ccaggaacgg tatacgccat ccccacgcca
    12481 gcagttcctc tttactgaga atgaagaaca taaaggccca gattgccgtt gcgctcaagg
    12541 ttccgcagtt agttcccata gctacaaatg aggagataat tccgcgctta ccttttggtg
    12601 catattccgc cagcatcgta ccggcaccgg aaatttccgc acctgcaccc aacccctgaa
    12661 taatacgcag cgtcaccagc aagatggggg caaaaacacc aatctgtgca taggtcggta
    12721 acacaccaat taaggtggta cagatcccca tcatggtgat ggtaataaag agcacttttt
    12781 tacgccctat tctgtcgccc attttgccga aaataaatgc tccgacaata cgcgccacat
    12841 aacctgcacc gtaggttccc attgccaaaa ttaacgccat tgccgttgat gattcaggaa
    12901 aaaatatttc atgaaacacc aacgctgcgc cgagcgaata taactggaaa tccataattc
    12961 acagatgttt tttctcattc tgtggtttcc ttggcgcttt ctaggttttt tcagatagtt
    13021 acattttttc aaaagcatcc tgagttcgat cttactatct atctgtggcc tatttctgtc
    13081 ccatatatgc cccaaaaaac accccaacag ataagcaatc ttttcatggt gttacgcgta
    13141 aaaacaataa cggcttgaaa ttattcaata cactctcgaa agtgcatcag ccaaccgcag
    13201 aacgttattg catacaacgt ttctgcggca tatcacgaaa cgattactta atgactggga
    13261 cttcagacca ctcaatatcc ggtacaatta tgtatcaacg cagtttaaca acaccctata
    13321 tttaatccag agtgcgagca acgttgtccc tttctctttt gcactcttaa aatatacgat
    13381 tacttaagtg aagatgcttg acgaatcgcc aaataaaaca tcttcatcag cataaaaatc
    13441 catatatg
//
LOCUS       DAPHDX010000120        13420 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPHDX010000120 DAPHDX010000000
VERSION     DAPHDX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.121"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            101..931
                     /locus_tag="RH058_003504"
     CDS             101..931
                     /locus_tag="RH058_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV3139039.1"
                     /translation="MSMPLSNAANNQFYINNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSLFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1072..1845)
                     /gene="uxuR"
                     /locus_tag="RH058_003505"
     CDS             complement(1072..1845)
                     /gene="uxuR"
                     /locus_tag="RH058_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV3139040.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2060..3520)
                     /locus_tag="RH058_003506"
     CDS             complement(2060..3520)
                     /locus_tag="RH058_003506"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV3139041.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3601..4785)
                     /gene="uxuA"
                     /locus_tag="RH058_003507"
     CDS             complement(3601..4785)
                     /gene="uxuA"
                     /locus_tag="RH058_003507"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV3139042.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5125..6468
                     /gene="gntP"
                     /locus_tag="RH058_003508"
     CDS             5125..6468
                     /gene="gntP"
                     /locus_tag="RH058_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV3139043.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISVVA"
     gene            complement(6718..7620)
                     /gene="fimH"
                     /locus_tag="RH058_003509"
     CDS             complement(6718..7620)
                     /gene="fimH"
                     /locus_tag="RH058_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV3139044.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7640..8143)
                     /gene="fimG"
                     /locus_tag="RH058_003510"
     CDS             complement(7640..8143)
                     /gene="fimG"
                     /locus_tag="RH058_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV3139045.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8156..8686)
                     /gene="fimF"
                     /locus_tag="RH058_003511"
     CDS             complement(8156..8686)
                     /gene="fimF"
                     /locus_tag="RH058_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV3139046.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8696..11332)
                     /gene="fimD"
                     /locus_tag="RH058_003512"
     CDS             complement(8696..11332)
                     /gene="fimD"
                     /locus_tag="RH058_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV3139047.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSIIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11399..12124)
                     /gene="fimC"
                     /locus_tag="RH058_003513"
     CDS             complement(11399..12124)
                     /gene="fimC"
                     /locus_tag="RH058_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV3139048.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12161..12700)
                     /locus_tag="RH058_003514"
     CDS             complement(12161..12700)
                     /locus_tag="RH058_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3139049.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMKFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12765..13313)
                     /gene="fimA"
                     /locus_tag="RH058_003515"
     CDS             complement(12765..13313)
                     /gene="fimA"
                     /locus_tag="RH058_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3139050.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3260 a         3473 c         3242 g         3445 t
ORIGIN      
        1 caacagaaac atgcatgtca tttgtttact tctcttttac tacagaaaaa tttatctatt
       61 tttctaaaca tcagtgaaat actcataaaa ggtaactatt atgtctatgc cattaagcaa
      121 tgccgctaat aaccaattct atataaataa tcacttcctt catcatccaa aagtcgacaa
      181 tgagttaaca cgtaaatatg aacgagctcg cctggatacg gaaaatatct atcttctgcc
      241 tcttgctcgt ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaaact
      301 tgatattagt aaagagtcct ggccatttaa ttatgtgaca gaagcatgca gagaatccga
      361 tggtattaca acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga
      421 cgaaatatat ggtggtatat gtaaaaaggc gcatgctgct acagaattag cagaaggttt
      481 acggctaaat ttatttatga agagcctttt cgatcctgtt gaggactata cagttcatga
      541 aataacatta ggccctggtt gcaatgttcc aggttacgcc ggaaccacga taggatatat
      601 ttcaaccctt ccagtttctc aggcaaaaag atggaccaat gagcagccaa gaattgatat
      661 ctatattgat cagataataa ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc
      721 cgcattactt aatgctaata ttggaatggg aaatgaccca ataattggta tagaagccta
      781 tcccggtacc gcggagatcc atgcaaaaat gggatataac gttatccccg gtgatgaaga
      841 tgcgccactc aaacgcatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa
      901 aaatggtgaa tggaattata ttggtaaata gccactgaga agaatgagta ctaatattca
      961 ggttatacag atatttaaac aataccctgc aatttacaaa gtaatactca ttgcgacttc
     1021 ttaatttaag aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcaggcgtc
     1081 gactttatcc agcggccatg aatcaaacag atagccgtca aaataaatat cgtcaacgtt
     1141 cgagaattcc agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc
     1201 cgctcgggca tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc
     1261 tttgcgatac aggctgtcat ccagatggct gtgcaactgg atccacattg ggttgttttc
     1321 ccgccactgc caggactgac ggaacagctc caccagcatg ctgttatgcg ttgcttcggc
     1381 aatagcgaga tggaactgca tgtcaccgct ttcgctgctg cccggcgcac tggaagccag
     1441 ttcacgctct tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg
     1501 cagggcggca aactcggcga tgttactttc cagtaactga cgcgcctgta acagctcaaa
     1561 aggacccgca tcattgcaga cgttggcatc cggactgtct gtgttgtggc tgcctgagct
     1621 atcaagaaca tagataccgg caccccggcg cacttccacc agccctttga tctccagcat
     1681 gatcagcgct tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc
     1741 cggcggcagc cgttcgccag ggttgtacgg cgtctttatg atcagatcgc ggatcatcgc
     1801 cccgacttcc tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt
     1861 aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg
     1921 ggcaatttta cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt
     1981 acaggtgggc aaaaaaacga aaactggtca accaaatacc agtttgattg accagtaagt
     2041 ctgtagggta atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca
     2101 gctgctgata tgccgcagtc accgcgccaa caaagtcggc attctgcggc agatcatcgg
     2161 caaaaatacc gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt
     2221 taatcttctg gaactcggcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat
     2281 ccacgccctg ggtgtaacgc atccagccag ccacgcccag cgccaggtga cgccagctgc
     2341 cgccgttttg caggtgcaga cgcaccgggt ccagcagacg ctgcggtaac ttctggctgc
     2401 cgtccattgc aatctgccag gtacggtgac gcagagatgg gttgctgaaa cgctcgatca
     2461 gcagcgtcgc ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt
     2521 cctgcatcat cagggcaaag gccgctttgc gataatccgg gttagtcatg gtgtcggcaa
     2581 tagtttcata gccgccgagg taaccgaggt acgccaggaa agagtggctg ccgttcagca
     2641 tacgcagctt catcatttcg aacggcacaa cgtctgcaac gaactgtgcg cccactttat
     2701 cccagtccgg gcgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc
     2761 aggcaatggc gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg
     2821 gcgtcgccgc tggaacgatg cggtcaacca tggtgcacgg gaaggtgaca ttttcttcaa
     2881 tccatgccgc cagctgcggg tcacgcgcct gagccagccc cagtaccgcg accttcgcca
     2941 catgaccgtt ttcacgcacg ttatcgcagg acatcaccgt aaacgctttc aacccttttt
     3001 cacgacgcag acgcaaggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt
     3061 tttctagatc gtgcttgatc agcgggttat tgagatccag ttgaccgctt gccgcatcag
     3121 cgcagtagcc tttttccgtg accgttagag agacaatcgc cgtttgcgga cgcgccatcg
     3181 cgttgagaat accttcgcag ccgtcgattt ccggatgcag cgcttctttc atcgaaccga
     3241 taattttcag ctcggtgctt tctgcgcctt tttccgctac ggtgtacagc agttgctgtt
     3301 ttttcaggtt ttcgatcagc acgcggtcgt tgcctggcat caggttaact tcgcagatgc
     3361 cccagtcgct gtcggtgctt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt
     3421 gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg
     3481 ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc
     3541 ttcagagagt gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtggata
     3601 ttaacggcta aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc
     3661 tttcagacga ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat
     3721 ctgatgaccg tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg
     3781 gtgttcttct tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg
     3841 cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa
     3901 gtaaatacgc ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc
     3961 gtaggaaccg gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca
     4021 ctgcatatct tcaatggtgg aaacaatgcg cggcaggccg aggatcgggc gcggcggatc
     4081 gtccgggtga accgccatac gcacgccaac ttcttcagca actggaataa tcgctttcag
     4141 gaagacggca aagttttcgc gcagcttcgc tttatcgata tctttgtaca gctccaggtg
     4201 cttacggaac tggtcgaggg tatacccttc ttccgcgccc ggcagaccgg caatgatgtt
     4261 acgcgtcaga cgcgctttgt cttcatcgct catagtggcg aagcgaacag cagcctgagc
     4321 aatttcttct tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa
     4381 tgcagcgaat tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc
     4441 gaggtcagta cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc
     4501 gcactgcgcc aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc
     4561 agtgtgggtt ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc
     4621 tgcgtcttca acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt
     4681 cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa
     4741 agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc
     4801 acatagtggg ttccacagca aagagacgcg gaaccctgga gttgtttagg tatccggatg
     4861 aacaaccaga ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt
     4921 tggtaagaca tgtcaacccg aattctgaaa ttggttaacc acatcacaag aatttcactc
     4981 tcctggttgt tgctgcaaat tgcagcaaaa ggttatcgat ggttattgat cagaggatgt
     5041 gacattcatc gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg
     5101 ttcaaccagg taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt agtattcggt
     5161 atcggcctga tgctggtact gaatttgaag ttcaaaatca attcaatggt ggctttgttg
     5221 gtggcggcgc tgtccgtcgg gatgctggcg ggcatggatt tgatgtcgct gctgcacact
     5281 atgaaagcgg gcttcggcaa cacgctgggg gaactggcta tcatcgtggt gttcggtgcg
     5341 gtcatcggta aattgatggt cgactccggc gcggctcacc agatagcgca tacgctgctg
     5401 gcgcgtctcg gtctgcgcta tgtacagctg tcggtgatta tcatcggctt gattttcggt
     5461 ctggcgatgt tctatgaagt ggcctttatc atgttagcgc cgctggttat tgttattgcc
     5521 gccgaagcta aaattccgtt cctgaaactg gcgatcccgg cagtagcagc tgccactacc
     5581 gcacattcac tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg
     5641 gatatgggga tggtttatat ctatggcgta ctggtgacga tcccaagtgt aatctgcgca
     5701 ggtctgatcc tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa
     5761 gcagatcaac cggtagatat gaataatctg ccctctttcg gcgtttcgat tctggtgccg
     5821 ctgatcccgg cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat
     5881 acccctgcct gggaagtggt taactttatc ggttcctcgc cgattgcaat gtttattgcg
     5941 atggtggttg cattcgtact ctttggcacc gcacgtggtc atgacatgca gtgggtgatg
     6001 aacgcttttg aaagcgcggt gaagagtatt gcaatggtga ttctgatcat cggtgcgggt
     6061 ggcgtgctga agcagaccat catcgacacc ggcattggcg acaccatcgg catgttgatg
     6121 tcccacggca atatctcgcc ctacatcatg gcatggctga tcactgtgct aattcgtctg
     6181 gcgacgggtc agggtgtcgt ttcggcgatg accgccgccg ggattatcag tgctgcaatc
     6241 ctagatccag caactggtca gctggttggc gtgaatccgg cgctgctggt gctggcgacg
     6301 gctgcgggtt ccaacaccct cacccacatt aacgatgcat ctttctggtt gttcaaaggt
     6361 tactttgacc tgtcagtaaa agacacgttg aaaacctggg gtctgctgga gctggtcaac
     6421 tccgtggttg ggctgattat tgtgctgatt attagcgtgg tagcgtaaaa gaacaccaga
     6481 gcctgccatt ggcaggctct gactgatgac aaagtctaaa aatgtgcccg gccagtcccc
     6541 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagcaaagtg cgctttgttt
     6601 atgccagatg cgacgttgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
     6661 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgccctgt gatttcttta
     6721 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
     6781 ggtacgtgca taatttgccg ttaatcccag acttaccgcc gaagtcccta ctgctcctaa
     6841 cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
     6901 ctgtgcaggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
     6961 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaatcgg
     7021 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacagcc
     7081 gccagtaggc accaccacat cattattggc gtaaatattc cacacaaact ggaaatcatc
     7141 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgagc cagctttaat
     7201 tgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
     7261 cgttctcgaa ttataaacaa cgcgcggcgt ttcgctggtg gtcggaaatg gatagctact
     7321 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccataag ccgagcctcg
     7381 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
     7441 cgaaagatct acgaccaggt tttgccccac attcacggca ggcgcaaggt ttacataaac
     7501 attagcgctg ccaccgccaa tagggatagc tgtaccattg gcggttttac aggcgaatga
     7561 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaataa ctcgtttcat
     7621 tacaatcatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
     7681 ttccctgagt ggctccgcca tttactgtca atgctctgac ctgtaacggg aagtgcgctg
     7741 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
     7801 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
     7861 tactgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
     7921 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacatc agactgaaag
     7981 aataaagatc gccgagatca accgtggcat tggtggtgga aactgtgcac ggtttggcga
     8041 cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg
     8101 ccaatattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
     8161 gatattcaag agtgaaggta gccgtggcat tgatatgccc tgcagtgaca ggcacctgtg
     8221 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
     8281 acgaaagcgc ggacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
     8341 gtattcccag tcccgaagcc gctgacaccg tattttcaag tgcaagcaga ttggcattgt
     8401 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
     8461 acagcaaaat acgaaatgga acaacaggag tcgtcgcgcc aatgttgtta aattgcttcg
     8521 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
     8581 tatccctgac atagccgcgg atagtaatcg tgctatccgc agccaaagcg tgactcaccg
     8641 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
     8701 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
     8761 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
     8821 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
     8881 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgggtcagc gtcatgagca
     8941 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
     9001 cgttagcaac cgcgttatct aaatcgacgt tatcagccag ggtattggta tccagcgcca
     9061 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
     9121 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
     9181 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccagt accccaccgc
     9241 tgactccgta atagagctgc ttaatatcat cactatggct gtaaccgata ttggcattgc
     9301 cgtaaccacc gcgataattc agcgtggcgt agcctgtgct tccgctatta ccatcgcctc
     9361 ccccggcata gccggtttgc acgctatagc tgaggttgtt gtcttccagc aacgtaccgt
     9421 atacaccagc cagattggtc attcgaccgt tgagatcgtg tgacatgctg tagctggcac
     9481 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
     9541 cgttaagcgc taacatctga tcacgtcctt tttgccaggc gtttttcgtc aggctatagc
     9601 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
     9661 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgagcgcc
     9721 cgagttgctg agtaacggtg agttgtaatt tcccgcgttt gttataagcg aggttgtaat
     9781 agtcggtgaa tttcggctta acctgaataa ctccgtcctg tgtttcgatg ttgtagccat
     9841 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
     9901 ccactaactg aatattcgtg cctgactcat tgagcgattt gttatagaga aaacgcaccg
     9961 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    10021 acagagcgcc cagtgccccc atgtttttcc cgataccgaa attaaaagca cgataacgat
    10081 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    10141 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    10201 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    10261 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    10321 cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta taaatgtcat
    10381 acccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg atcaccgggg
    10441 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    10501 agttaatacc atcgaaaata tcgccctgag tataaccatc acccagcgtc agccgggaac
    10561 gtaacggtat tatgtctcgc tcaagccagg tattgatatg ctgccattta tttttgctac
    10621 ctgatgatct gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    10681 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    10741 tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata ccgggatccc
    10801 ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga gggatcgtca
    10861 ggttgagtcg ctgctgacca acatctaaat gcgcagtagc gtcatggatt attgaggtta
    10921 atggcacgca ggcatcatcc gccagcagat tcataccgga gacagaagcc gtattcagcc
    10981 ccatactggc aagttgcgcg cgtgtcaggc agggaacaat cccttgttcg ctgtcgcccg
    11041 tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata tcgacacgat
    11101 acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcagcc acagcctggg
    11161 gatcatccgc taaaaagcgc gggttaaaat agagttcggc agatgacaaa ggtgcctgtg
    11221 cggcaaaagc acaggcaaca acgagccgga caaaaaaacc agccaaacga tgcttacgaa
    11281 tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac attattcctc
    11341 cggtttaaaa tcacccggca gtcaattctt tttattcagg cgaaaaattc cccctgcgtt
    11401 attccattac gcccgtcatt ttgggggtaa gtgcgccata atcatttatt gttcggtaag
    11461 taatattgct tcccgcatca gaaggcaatt taaccgtgct ttcgcccatt ggaggcacca
    11521 aggcattttc aagaacccgg gctccggcat tcaactctgt taccgtcagg taatagggtg
    11581 tcgggttaat cagcgtcaga gaattcgcgc tacgacgaaa tcttaatttt tctgcggcct
    11641 gatcgggtgg caacgctaat ttagccgggc gatagtacag tttaatgcgg ctgataattg
    11701 cgagctgtag cgtattctca gtcaattttg atttatccat tgacggaatc gctttaacat
    11761 tcatccagaa taaactttcc cgatcctgtg gcaattggtt atttgttgca tcaagaatac
    11821 gtaaggtatt ctcttttttt cccttcatcg caaacagagg aggcgtcacg ataaaacgac
    11881 catcctttac accatcggca ttttccaccc atgattgaat taaataggta ctattttcat
    11941 cattatttgt cacggcaagt tgcacttgtt tttgccctgc cggataaatt acgcgagtcg
    12001 cacctaaggc cactcctgct tcagcgcgtc cggcaaccat cattgccatg aacatcagga
    12061 tacctgccag caagcagaat gttatttcct gcgatttcct tacattgacg tttttattac
    12121 tcactgtcct gttcctgtta tcacattatc tgctgaacaa ttactgatag gttaaagaga
    12181 accaggcctg ggcattggcg atgccgccag taacccgacg cccggtagca cgatatttgg
    12241 cgatgaaatg tagcgaagta gagcctgagt aaagccgttt ccagtttgct ggaggacgat
    12301 taatcggtac gaggtttcct tcatcatcaa acaacgctac gccaatattg gtggctatcc
    12361 ctggcccctc tcccacggaa agcacatccg gatttttacc atccgcgaca ccgtgaaacg
    12421 ccacacctac acgttcactc accaccgtgc tacattcccg taaatgaata acaaaaggca
    12481 ccggtgcgct atcttcccct accgcatgaa accggttact gctgatttgc cccatattga
    12541 ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg acgccctgaa
    12601 atttcatatt tccgccgggc agcgtggtat tccatttatt tccggccaga gcaaacattg
    12661 gcagcaacga cgctaataga aataatctct tccttttcat cgttattttt atcgcacaag
    12721 ggtgggcatc cctgcccgta atgacgtccc tgaacctggg taggttattg atactgaacc
    12781 ttgaaggtcg catccgcatt agctgcgcca ggggttgcgg caccggttgc aaaataacgc
    12841 gcctggaacg gaatggtgtt ggttccgtta ttcagggttg tttgtgcact aaatgtcgca
    12901 ccgtccagcg ccagcgcagc gcccgttctg tccaggatct gcacaccaac ttttgttgcg
    12961 ctacccgcag ctgaactctg caaagctaat acgttaggat gagtgccatc aattgcggta
    13021 ccaaggaagg caatagctgc tttgtctgca acagtagtgt cacaatcgtt cagttgaatg
    13081 ttaaaaccga cagcagaact ggttgctcct tcttgtgcca gcgatgcggt acgaacctgt
    13141 cctaactgaa cggtttgatc aacagagcct gcatcaactg cgcaagcggc attaacaact
    13201 tcccctttaa agtgaacggt cccaccattt accgtcgtag tatcggccag agccgctgcg
    13261 gaactgaggg acagagccga cagaacaacg attgccagag ttttaatttt catgctgctt
    13321 tcctttaaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac tttgttttgt
    13381 caacgagttt gactgccaac actgcacagt tttcccccaa
//
LOCUS       DAPHDX010000121        13225 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPHDX010000121 DAPHDX010000000
VERSION     DAPHDX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.122"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..661
                     /locus_tag="RH058_003516"
     CDS             <1..661
                     /locus_tag="RH058_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV3139051.1"
                     /translation="IKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMA
                     EVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTW
                     LTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQG
                     MGISILRGEENIPIDSDTLVTRQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHA
                     D"
     gene            complement(815..2464)
                     /locus_tag="RH058_003517"
     CDS             complement(815..2464)
                     /locus_tag="RH058_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV3139052.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2615..3964)
                     /gene="ghxP"
                     /locus_tag="RH058_003518"
                     /gene_synonym="yjcD"
     CDS             complement(2615..3964)
                     /gene="ghxP"
                     /locus_tag="RH058_003518"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV3139053.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4510..4974)
                     /gene="soxR"
                     /locus_tag="RH058_003519"
     CDS             complement(4510..4974)
                     /gene="soxR"
                     /locus_tag="RH058_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV3139054.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5060..5383
                     /gene="soxS"
                     /locus_tag="RH058_003520"
     CDS             5060..5383
                     /gene="soxS"
                     /locus_tag="RH058_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV3139055.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5386..6972)
                     /gene="pdeC"
                     /locus_tag="RH058_003521"
     CDS             complement(5386..6972)
                     /gene="pdeC"
                     /locus_tag="RH058_003521"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV3139056.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7402..7683
                     /locus_tag="RH058_003522"
     CDS             7402..7683
                     /locus_tag="RH058_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV3139057.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7782..8318)
                     /gene="ssb1"
                     /locus_tag="RH058_003523"
     CDS             complement(7782..8318)
                     /gene="ssb1"
                     /locus_tag="RH058_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV3139058.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8572..11394
                     /gene="uvrA"
                     /locus_tag="RH058_003524"
     CDS             8572..11394
                     /gene="uvrA"
                     /locus_tag="RH058_003524"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV3139059.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11429..11785)
                     /locus_tag="RH058_003525"
     CDS             complement(11429..11785)
                     /locus_tag="RH058_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV3139060.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11789..12205)
                     /locus_tag="RH058_003526"
     CDS             complement(11789..12205)
                     /locus_tag="RH058_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV3139061.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12316..13029)
                     /gene="aphA"
                     /locus_tag="RH058_003527"
     CDS             complement(12316..13029)
                     /gene="aphA"
                     /locus_tag="RH058_003527"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV3139062.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3432 a         3454 c         3230 g         3109 t
ORIGIN      
        1 gataaaaatg gccgacggaa ctctaaatgc atgctccgct atgcatgttc aattctacaa
       61 tgcagcaatg aatagagcca atattaaaaa tacctttctt gactattcaa atttttatat
      121 ggcgtacatg gctgaggtaa atctttataa agtaatagcg ccatatgtta atttatttaa
      181 agccgacctt agtttctcta aactcgattt aattaacttt gaacatgctg atctgtctcg
      241 cgtcaatctg aacaaagcaa tcctccagaa tataaactta attgatagca aactcttttg
      301 tacgtggcta acaaatacat tcctcgaaat ggttatatgt accgactcta atatggctaa
      361 tgttaatttt aataatgcca atttaagcaa ctgccatttc aactgttcta ttttaacaaa
      421 agcctgtatg tttaataccc gtctctatcg ggttaatttt gatgaggcta gcgtccaggg
      481 aatgggcatt tccatactcc gtggggagga aaatatcccc attgatagtg ataccctggt
      541 aacacgacag aaattctttg aagaagattg tacctctcat actggcatgt cacaaactga
      601 ggataatatt aatgcagtcg ctatgaagat tactgcagat attatgcaac acgcagattg
      661 aggttaaatc gaagaacagc caatgaattg actttacaag agagataact tatttgtttt
      721 agaatcatga atgttagccc gagctaacct gttaaaggtg caaaagattg cacctttttc
      781 agattacctc gggcctggtt acgtcgcttt cggcttactg attttcctca attagcaacg
      841 cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt ttcgttgctg atctcccgcg
      901 tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa tgccgccaga cggaagcgac
      961 gctcaaggtt ctcttcctgc acggaacttt caacgtcgtt acgtccatcg gcgcgacgac
     1021 gcaggttacc aatgacgcga gaactgacct ccgtaagcag ctggttatcg atattctctt
     1081 cggtatccgc cgccagacgc tcctccattt tctggatcgc cacaatcgcc acttctgccg
     1141 ttgccgctcg cgcaatacgt tcctctttca attgttgcga atgatccgcg acttcaatgt
     1201 gttgtagcag aataggcaac atcaccacgc cgacaaacag cgagaagaga atgacgccag
     1261 ccgccaggaa caccagctca tagcgcgccg ggaagacgtt accatccggc aagagcagcg
     1321 ggatagagag cacaccggcc agagtgatcg ccccacgcac cccagcgaaa gacgcgatca
     1381 ggatttctcg tgtggtccac gaaccaaact ccatcggctt cttcttcagg aagcggttgc
     1441 tgaacttttt catcgtccac agccagccga aacggaccag catcagcgcc gcatatatca
     1501 gaataatatc ggtaaacagc atccagattt cgacgttagg gtcgatttct gccgccatca
     1561 gcgacgtctc cagaataccc ggtagctgca gacctaacag caggaacacc atgccgttaa
     1621 agacaaattc cagcatcgcc caggtgctgt ttgcacgcag gcgcattgcc agcggcgcac
     1681 ggcgcatcac accggagcgg gtgatggtca tcccggcggc aaccgcagcg aggatgccgg
     1741 aaacgccaat atgttcggca atcagataag aagcgaatgg cagcaggaac agcaggacga
     1801 tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa tcgcagcgag cgaccgtaca
     1861 gccagctaac cacaaaaccg gcgagaatac cgccaatggc tactttcata aattcgaccg
     1921 tcgcaccgcc gacggtgaag atcatcgtcc ccatcgccac tgctacggca aacttcaacg
     1981 acaccaggcc ggaagcgtca ttcatcaacg cttcgccctg caaaatgccc atgatttttt
     2041 tcgggatacg cccttcgcca acaatcccgg agagcgccac agcatcggtc ggagaaagta
     2101 ccgccgccag cgcaaaggcg gggatcagcg gaatgcccgg caccacccag taaataagga
     2161 agccaatgcc gaccacggtg accaccacca gcgccagcgc gaggccaaaa atctctcgac
     2221 catgttcgag aaattcacgg gtcggcgttt tccagccatc agcgaacagc aacggcggga
     2281 taaacaagac taaaaagagt tcaggatcaa actccacatg caaaccaaac gtcggccacg
     2341 ccagtagcgc accgatggcg atttgcataa gcggaagcgg gatctgaaag ggcatgacac
     2401 gagtgaccac cccggacagc gagaccacga gggtcattat cagtatggtg aagaagattt
     2461 ccatgcgttc cctgtttgcg atgtttttta tgatctgaga aagacgttat gcctgaattc
     2521 tcattttttc agaatatcgc aaaagctcac acaatgtgcg gagaaaataa aaacgggcag
     2581 ccattgccac ccgttttcag aatccgatga aagattagat agcccagcca cccgcataga
     2641 aggtcaccag cgccacggcg ataacgaccg taccgatgtt caacttgcgc cattcgccgg
     2701 aaaccagacg accaatcacc agagtcgcga agccgatcat gatgcctgtt acgatgttac
     2761 aggtcagcac gatgaatacc gccgtaacca gacccgccat cgcatcaaca aaatcagcaa
     2821 agtcgatttt cgccacgttg ctcagcatca gcaggccaac gtacatcagc gccggagccg
     2881 ttgcataccc cggaacgagg taagagagcg gagagaggaa cagaatcagc aggaacagca
     2941 cgccaacggt gatagccgtc aaaccggttt taccgcccgc cgccgtaccc gccgcagact
     3001 cgatgtatac cgctgccgga gctgcaccca ccaggccaga gaaaacgctg ctcatggagt
     3061 cagtggtcag tgctttccca ccgtcgatga tctgcccatc tttatccagc aggttcgcct
     3121 ggccggcgac ggcacggata gttccggtgg catcaaatac tgccgtcatc accagcgcca
     3181 gaacgcttgg caggactaca ggattcagcg cgcccataat gtccaggctg ccaatcagtg
     3241 aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac gccggagaaa tggacgttag
     3301 gatcgaagat caaaccgaca attgagatac cgataatggt cagcagaatg ccaccaggga
     3361 ctttcagttt ttccaggccg atgatcaccg ccagacctac cagtgacata atcaccggga
     3421 aggtcgcgaa atcacccagc gcaacgggca gaccatcaag cgggttttta atcaccagac
     3481 cgacaccatt agcggcaatg agcagcagga acagaccgat accgatcccc gtgccgtgcg
     3541 ccacaccgtg aggcaagttg cgcaaaatcc agctacggat acccgtggca gaaattaccg
     3601 taaacagcac acccatcagg aacacggcac ccagcgcgac aggtacgcta atatgttgcc
     3661 ccagcaccag gctgaatgcg gtaaatgccg tcagggagat ggcgcaacca atcgccaacg
     3721 gcagattagc ccacagaccc atcacgatag aaccgagtcc ggcaaccaga caggttgcaa
     3781 cgaaaactgc cgcaggcggg aagcctgctt tacccaacat acctggaacg acgatgaccg
     3841 agtagaccat cgccagaaac gttgttaacc cggcaaccac ttcctgacgg acagtgcttc
     3901 cacgttgtga aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct gatggcgtag
     3961 acataagaaa acatcccctg agagtttaat tttcgtcaag atgcgtggcg gacaatcccc
     4021 tgccagcgaa acgttatctt cctgctccag gtttgcgaca atttgctgcg tcgcaaaaaa
     4081 gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa aaagcaaacg attatccggc
     4141 gatcatagcg gctttttcaa ccgaagtttg ctgctttttt ctcttttatt gtcttgacga
     4201 tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcggt gcgcaagatt gcagcgttat
     4261 cgagccgtat accgttcagg attgcgataa aaaatcgtct gagatgacaa aaatattgcg
     4321 gttaaaaagt atacttttac actggcgaag aaacgtcggt gacatacaat taaagcatca
     4381 acaccaaccg gaacctccac cacgtgctcg aatgaggtgt gttgacgtcg ggggaaaccc
     4441 tcctgtgtac cagcgggata gagagaaaga caaagaccgg aaaacaaact aaagcgccct
     4501 tgtggcgctt tagttttgtt catcttccag caagcgtgcg ccggtacctt cttctcctaa
     4561 gcggtcgccc gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca
     4621 tccgtccagt tcgtcacgca gcgctactaa ggtatgaatg cgccgatcca actcttctcg
     4681 ccattgggac gaaagctgtt tccactcttt cgcacttaac gtatgccctt cgggcaacac
     4741 gccaaacgct tcaccaatgg tcgccagcgg aatgccaata cgctgagcaa ttttgataat
     4801 tgcaacatat cgcaacacat cacgtttata tcgccgctga ttgccgctgt tacggatact
     4861 ggtaatcaac cctttacttt catagaaatg cagcgccgat accgccacac cgctgcgttt
     4921 cgccacttcg ccgggggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg
     4981 ctttacctca agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac
     5041 actgaaaaga ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat
     5101 tgacgagcat attgaccagc cgcttaacat tgatgtagtc gcaaaaaaat caggctattc
     5161 aaagtggtac ttgcaacgaa tgttccgcac ggtgacgcat cagacgcttg gcgattacat
     5221 tcgccaacgc cgcctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt
     5281 tgatatcgca atggacctgg gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg
     5341 gcagtttgat cgcactccca gcgattatcg ccaccgcctg taattttatt gcccgcgcgt
     5401 taactcccgc gcgggtaatt gctccatcca ttgcataaac acctgcggcg gcatcgcctt
     5461 cgcaaagaac catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgctc
     5521 ctcagtttcg acgccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat
     5581 gtgttccgca atcaaatgac tggttttgtg ggtggtcagc gtttcaacaa acgatttgtc
     5641 gattttcaaa atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc
     5701 aaaatcatca attgccactt cgtaacctgc ctggcggaaa gccagaataa tcggcgtcat
     5761 tttgtcaaca tcaagaaatg catgttcagt cacttcaaat ttaatttgct gcggacgcac
     5821 cgcgtattgc tctgttttct gattgattcg cgctatcaac cgtgacgtat gaaaatcgga
     5881 ggccgacagg ttaatagaaa catagcgatc tgcatgtgtt gccaggtaat cgcccagatc
     5941 gcggaagaca ttatcaataa cataatcagt tacctgttct atcatcccct ccttttctgc
     6001 cagcggaata aactctgccg gattcattat ttgcccctgc tcaccaagcc aacgtaacaa
     6061 cgcttcagcg ccgatacatt tttctgtttt gatatcgatt attggctggt aataaagaca
     6121 aagttgatgt ttttcgaggg cgcgttgcaa tttacgtttg ggagataaat agttttgtcg
     6181 aatacgtagc cagagtaata gcagaacaag actccccaaa ataccggcgg gcaacgcaaa
     6241 aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc
     6301 aattgggcgt tttgtcgaat aaactgtcgc atataaatag ccatttcttt gtacggttaa
     6361 atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa
     6421 aaaggttttc gtcaccgtat catacacacc ccattgcaat gtcgggtcat cagacatcac
     6481 ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat
     6541 tttatagcca gagaaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg
     6601 cttataatcg gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa
     6661 aaaatggtta tcacgggcat agatcaattc attaatataa agatagccac gaataatatt
     6721 caacattcgc ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc
     6781 gctcactaaa tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa
     6841 ggttcggagc tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag
     6901 aatgatggga aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc
     6961 acgatgactc attgctatca atttcctgcc tgtttatacc cggccattgc aaggtgaaaa
     7021 ccctgggtac cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt
     7081 gaaattgata cctttctaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta
     7141 atttcctaca ggaatgcaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca
     7201 cataatctgt acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga
     7261 tatagttcac aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc
     7321 gcgatagcca gagtgtacca cgcctcccgt gaacaacgcc gcgctgtcca gggcatcggc
     7381 tcttttcaga ggaattgttt gatggctacc ctcaccactg gcgtggttct tcttcgctgg
     7441 caacttctta gtgccgtaat gatgtttctg gccagcacac tcaacatccg ttttcgtcgg
     7501 tctgattatg tcgggcttgc ggtgatcagc agcggtctgg gcgttgtttc cgcctgttgg
     7561 ttcgcaatgg ggttgcttgg catcacaatg gcggacatca ccgctatctg gcataacatc
     7621 gagtcggtga tgatagaaga gatgaatcag acaccgccac aatggccaat gattttgacc
     7681 tgatacgtag aagcctccat aacggaggct tcttttttat ggctggcaga tgctttaatc
     7741 atccacctta aaacaatata acctattgtt ttaatgacaa atcagaacgg aatgtcatca
     7801 tcaaagtcca tcggcggctc gttagacggc gctgccggag cggactgctg cgggcgagac
     7861 tgcgcgccgc cgctgaactg attgccaccc tgcggctgct gaggctgacc ccaaccgccc
     7921 tgcggctgac caccaccgat attaccacct gccggagcgc caccaccctg acgaccaccc
     7981 agcatctgca tggtgccgcc aacgttcacc acgacttctg tggtgtagcg atcctgaccg
     8041 gattgatcgg tccatttacg ggtacgcagc tgaccttcga tataaacctg agaaccttta
     8101 cgcagatatt cgctggccac ttctgccagt ttgccgaaca gcacaacgcg gtgccattca
     8161 gtctgttctt tcatctcgcc ggtcgcttta tcacgccagg attcggaagt agccagcgta
     8221 atgttggcaa ctgcgccacc atttggcatg tagcgtactt ccgggtcctg acccagatta
     8281 ccaacgagaa taaccttgtt tacgcctctg ctggccatgt tcgtgtctcc tgaaaaaaat
     8341 cgttctgaat aagtgtaaac gcgcgattgt accattacca atagcgcttt tactatgttg
     8401 tgacctcggt tccgggaaac aaacctggcc agacattgtt acacaacact ccgggtaatg
     8461 cattccaata ctgtatattc attcaggtca atttgtgtca taattaaccg tttgtgatcg
     8521 ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc gggaaaggtg aatggataag
     8581 atcgaagttc ggggcgcccg cacccataat ctcaaaaaca tcaacctcgt tatcccccgc
     8641 gacaagctca ttgtcgtgac cgggctttcg ggttctggca aatcctcgct cgctttcgac
     8701 accttatatg ccgaagggca gcgccgttac gttgaatccc tttccgccta cgcgcggcag
     8761 tttctgtcac tgatggaaaa gccggacgtc gatcatattg aggggctttc tcctgccatc
     8821 tcaattgagc agaaatcgac gtctcataac ccgcgttcta cggtggggac aatcaccgaa
     8881 attcacgact atttgcgttt gttgttcgcc cgcgtcggcg aaccgcgctg tccggaccac
     8941 gacgtcccgc tggcggcgca aaccgtcagc cagatggtgg ataacgtgct gtcgcagccg
     9001 gaaggcaagc gtctgatgct gctcgcgcca atcattaaag agcgcaaagg cgaacacacc
     9061 aaaacgctgg agaacctggc aagccagggt tacatccgtg ctcgtattga tggcgaagtc
     9121 tgcgatcttt ccgatccgcc gaaactggaa ctgcaaaaga aacataccat tgaagtggtg
     9181 gttgatcgct tcaaggtgcg tgacgatctt acccaacgtc ttgcggagtc gtttgaaacc
     9241 gcgctggagc tttccggtgg tacagcggta gtggcggata tggacgaccc gaaagcggaa
     9301 gagctgctgt tctccgccaa cttcgcctgc ccaatttgcg gctacagtat gcgtgaactg
     9361 gagccgcgac tgttttcgtt taacaacccg gcaggtgcct gcccgacctg tgacggcctt
     9421 ggcgtacagc aatatttcga tcctgaccgc gtgatccaaa accccgagct gtcactggct
     9481 ggcggtgcga tccgtggctg ggatcgccgc aacttctatt acttccagat gctgaaatcg
     9541 ctggcagatc actataagtt cgacgtcgaa gcgccgtggg gcagcctgag cgcgaacgtg
     9601 cataaagtgg tgttgtacgg ttctggcaaa gaaaacattg aattcaaata catgaacgat
     9661 cgtggcgata cctccatccg tcgtcatccg ttcgaaggcg tgctgcacaa tatggagcgc
     9721 cgttataaag agacagaatc cagtgcggta cgtgaagaat tagccaagtt tatcagcaat
     9781 cgcccatgcg ccagctgcga agggacgcgt ctgcgtcggg aagcgcgcca cgtttatgtc
     9841 gagaatacgc cgctgcctgc tatctccgac atgagcatcg gtcatgcgat ggaattcttc
     9901 aacaatctca aactcgctgg tcaacgggcg aagattgcgg aaaaaatcct taaagagatc
     9961 ggcgatcgtt tgaaattcct cgttaacgtc ggcctgaatt acctgacgct ttcccgctcg
    10021 gcagaaacgc tttccggcgg tgaagcccag cgtatccgtc tggcgagcca gattggtgcg
    10081 ggcctggttg gcgttatgta cgtgctggac gagccatcta tcggcctgca ccagcgcgat
    10141 aacgagcgcc tgttgggtac gcttatccat ctgcgcgatc tcggtaatac cgtgattgtg
    10201 gtggagcacg acgaagacgc aattcgcgcc gctgaccatg tgatcgacat tggcccgggc
    10261 gcaggtgttc acggcggtga agtggtcgca gaaggtccgc tggaagcgat tatggcggtg
    10321 ccggagtcgt tgaccgggca gtacatgagc ggcaaacgca agattgaagt gccgaagaaa
    10381 cgcgttccgg cgaatccgga aaaagtgctg aagctgacag gcgcacgcgg caacaacctg
    10441 aaggacgtga cgctgacgct gccggtgggt ctgtttacct gcatcaccgg ggtttcaggt
    10501 tccggtaaat cgacgctgat taacgacaca ctgttcccga ttgcccaacg ccagttgaat
    10561 ggggcgacca tcgccgaacc agcaccgtat cgcgatattc aggggctgga gcatttcgat
    10621 aaagtgatcg atatcgacca aagcccaatt ggtcgtactc cacgttctaa cccggcgacc
    10681 tataccggcg tgtttacgcc tgtgcgcgaa ctgtttgcgg gcgtaccgga atcccgtgcg
    10741 cgtggttata cgccaggacg tttcagcttt aacgtccgtg gcgggcgctg cgaagcctgt
    10801 cagggcgacg gtgtgatcaa agtagagatg cacttcctgc cggatatcta cgtgccgtgc
    10861 gatcagtgta aaggtaaacg ctataaccgt gaaacgctgg aaattaagta caaaggcaaa
    10921 accatccacg aagtgctgga tatgaccatc gaagaggcgc gtgagttctt tgatgccgta
    10981 cctgcactgg cgcgtaagct gcaaacgttg atggacgttg gcctgacgta cattcgcctg
    11041 gggcagtccg caaccacact ttctggtggt gaagcccagc gcgtgaagct ggcgcgtgag
    11101 ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg acgagccgac caccggtctg
    11161 cacttcgccg atattcagca actgctcgac gtactgcata aactgcgcga tcagggcaac
    11221 accattgtgg tgattgagca caatctcgac gtgatcaaaa ccgctgactg gattgtcgac
    11281 ctgggaccag aaggcggcag tggcggcggc gaaatcctcg tctccggtac gccagaaacc
    11341 gtcgcggagt gcgaagcttc gcatacggca cgcttcctca agccgatgct gtaatcgtta
    11401 aggccgcttt ctgagcggcc ttttcctttc agagttgtac cagcaattta cgcttttctt
    11461 ccggtagtaa attcactgcc tgctgataag acgcatccac cagataatag atttgcgaat
    11521 ccggcagcga accgtcgaga tacacggtgc tccagtgcgc tttattcaga tggcggctag
    11581 gacgcacatc gctgtgctgc tgacgtagca gctccgccag ctccgggctg gttttcagcg
    11641 aaacagctgg gcgattttct acttctttca ccatcgcaaa cagtacatct tccactttga
    11701 tctgcgtcgc tttccagtcg ttatgcacgc tctgttctgc gcctggtttt gccatgcaat
    11761 attgtagcaa ctccgaaatg gtcatttttt actccccttg tagtgtcgcg atgatgcgac
    11821 gcgatccgcc gtggatgcga tgttccccca gccagatgcc ttgccaggtg ccggtctgaa
    11881 tacgcccttt atgtaccggc aatacaagcg atgttcccag cattgaggat ttgatatgag
    11941 aaggcatatc gtctgctccc tcatagtcat gctcataatt tccgttgtcg ggaacggtgc
    12001 ggaggaaaaa acgctccatg tcgtggcgta cggtgggatc gcagttctca ttaagtgtca
    12061 gagaggcgga ggtatgttgc agcaacagat gcagtaagcc gatgttaacg cgcggcatat
    12121 cagccagctg attcagaatt tcatccgtta ccagatgaaa cccacgagat ttggcgctaa
    12181 gcgtgagcgt cttttgatac cacatgtgct gctccttgat aaaactctct taatcagttt
    12241 gcagcaagac agcgaaagga taaaggtgtg attaaaaaaa cagcattcgg gagagcgtca
    12301 cgctctcccg ctctgtcagt attctgaatt gacgatcacc tcttcaccaa acgcacccgc
    12361 ttgtggcaag ggtttgtagg tagagttgga ggcgcgcaga atgcggatac cacgagcgcc
    12421 gacatcgcgt gcggcggtaa tatcgttatc ggaatcgcca taaaaaattc ggatattttt
    12481 atcctgcagc cattgcgatt ttgtattttg ccctggttta tcgcccgcaa agatcaccgg
    12541 attcatgttg gtgacaggaa tatgaaaatt atccgccagc gtttttgaaa ccgtttctgt
    12601 tttcgtcggg ctacgaccag tcacaaagaa gatcgcgtca ccgcggcgta catgcatatc
    12661 aatcagctgg cgagcgacct cttttggaat gctgaattca tcccagccat tgttcatttt
    12721 ttcccagaac acaggatttt tcagataatc ttcgctttct ggtgagaagg tttttttgcc
    12781 gcgccagaag cccgggctgg aaaaaagtac cgtgtcatcg atatcaaacc ccaccgccat
    12841 tggcggacgc cctgcgaggc tattttcaat ttgtgcgacc gaaacccaat gaattggtgc
    12901 ctgttcagca agcctggcaa cgttagtccc agggttaagc ggtgaaggag atgaggccag
    12961 ggcaacagcg gaactgttta gagcgaacaa taagcaaacg gcactgattg cctgtgtgat
    13021 cttgcgcata tttttcccta aatagtcagt atgttgaaac tttttattgt gagatttgtt
    13081 gcaaaaacca tctgaccata acgccagctg ccggggaaag gaaggattat ctgcggtttt
    13141 tgtgatgaaa agcaggatga ttatgtttaa cccagtaaat acgttcctgt ctttttttgt
    13201 ataaaaaatg tttattactc gatgt
//
LOCUS       DAPHDX010000122        13189 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPHDX010000122 DAPHDX010000000
VERSION     DAPHDX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.123"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            183..899
                     /gene="nanC"
                     /locus_tag="RH058_003528"
     CDS             183..899
                     /gene="nanC"
                     /locus_tag="RH058_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV3139063.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            919..2025
                     /gene="nanM"
                     /locus_tag="RH058_003529"
     CDS             919..2025
                     /gene="nanM"
                     /locus_tag="RH058_003529"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV3139064.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2090..3070
                     /locus_tag="RH058_003530"
     CDS             2090..3070
                     /locus_tag="RH058_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV3139065.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(3078..3200)
                     /locus_tag="RH058_003531"
     CDS             complement(3078..3200)
                     /locus_tag="RH058_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3139066.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            3282..4154
                     /locus_tag="RH058_003532"
     CDS             3282..4154
                     /locus_tag="RH058_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3139067.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            4324..6399
                     /locus_tag="RH058_003533"
     CDS             4324..6399
                     /locus_tag="RH058_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV3139068.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            6392..7735
                     /locus_tag="RH058_003534"
     CDS             6392..7735
                     /locus_tag="RH058_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDV3139069.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAATYSLVKHGLKDCFKLRPDLLIQSHKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     AQHFAFSETLKLWVVPYRIMAKRGERMMWPNDVSCTMSPKLDRMAASVSYSGGMR"
     gene            7732..8118
                     /locus_tag="RH058_003535"
     CDS             7732..8118
                     /locus_tag="RH058_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139070.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            8229..9368
                     /locus_tag="RH058_003536"
     CDS             8229..9368
                     /locus_tag="RH058_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139071.1"
                     /translation="MVKQHSEFTQPLVELPGSAGRLLKQVRDIEQVAPLFRDAGLVKA
                     VPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDSVSELDD
                     IDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLERNVRHKQ
                     EYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTEQGRKQI
                     LASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRIEPNIEA
                     KQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNAMPLPQL
                     LGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            9358..11043
                     /locus_tag="RH058_003537"
     CDS             9358..11043
                     /locus_tag="RH058_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3139072.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRSADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            11180..11593
                     /locus_tag="RH058_003538"
     CDS             11180..11593
                     /locus_tag="RH058_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139073.1"
                     /translation="MAVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAK
                     GQQKLEEKLKNLLEAAQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGT
                     QVIAAPDVEVVFSRFLFIKNLNMIVLDRIHYEVLM"
     gene            11590..13059
                     /locus_tag="RH058_003539"
     CDS             11590..13059
                     /locus_tag="RH058_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV3139074.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
BASE COUNT         3790 a         2825 c         3113 g         3461 t
ORIGIN      
        1 tgtttattta aataatgtga aaagagattt ttcacaggag accttataca aaaaaatata
       61 aaatacagct accggttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat
      121 aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat
      181 ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa
      241 tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg
      301 agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta
      361 atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg
      421 ttgaagttaa ttacgcgatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa
      481 cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga
      541 caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag
      601 acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc
      661 atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaacgatt
      721 atcgctatgc aaaccataag aaatgggcga cggaaaatgc atttgttcta caataccata
      781 tgacgcccga tattacgcca tacatagaat atgactacct ggaccgtcag ggtgtttaca
      841 acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt
      901 agacaggaga cagtcacaat gaataaaaca ataacggcgc ttgctatcat gatggcttca
      961 tttgccgcaa acgcgtctgt attacctgaa actcctgtgc catttaaaag tggtaccgga
     1021 gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacggc atggtacaag
     1081 ctggatacac aggccaaaga taaaaaatgg acagcgttag ctgcattccc tggtggacca
     1141 agagatcaag caacctcggc atttattgat ggcaatctgt atgtgtttgg cggcattggc
     1201 aaaaatagca agggcttgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc
     1261 aatagttggg ttaaattgat gtcgcatgca ccgatgggca tggcgggtca tgtgactttt
     1321 gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat
     1381 tttgaagatc tcaacgaggc tggaaaagat tcagcaacta tagacaagat caatgcccat
     1441 tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc
     1501 tcaacacagc aatggagtta cgctggcgaa tctccctggt acggaacggc tggtgcggcg
     1561 cttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga
     1621 acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgct
     1681 cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt
     1741 atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac
     1801 tatgcgcatg aaggcctaaa aaaatcatat agcactgata ttcatctttg gcataacggg
     1861 aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg
     1921 aataatagtc tattgattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca
     1981 gttttgatct ctgtgaagga taataaagtc acagtacaaa attaacgctt cagggccccg
     2041 gtaaaggggc cctgctatca atttgtcatt tcaattacgc gaaatttata tgaacgcaat
     2101 aatatcgccc gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta
     2161 tggcgaagga ctgccattac cggacaggga agatgcgcct catcccagaa ttaaacaatt
     2221 agcgagattt gcgcatacgc atcccggagg cccgtcatgt cactttaacg acattattcc
     2281 actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac
     2341 gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact
     2401 gccctctatt cctgataatg caggggttct catcgttcca tgttgtcgtg gcggctcggc
     2461 ttttatcgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg
     2521 tcgttgggga tcggatactc cgctatacca ggatttagtc agcagaacgc gagccgcact
     2581 ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt
     2641 aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg
     2701 tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg
     2761 cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg
     2821 caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga
     2881 aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg
     2941 ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac
     3001 ggcagcccgt cggggggtta tttctgacag gtttgtagaa gcaattttgc agttttggcg
     3061 cgaaaagtga gcgttcatta atttgattta ttcgcatcca tcaacgcttt taactcctcc
     3121 ggcgtaaaag gtggtttgcg tcggtgcaac atcgcatggc aattagggta cacaggcagc
     3181 aaaaaaacaa atgagatcaa agtaatatat cttcacttgc aggcttgaac agccgaaaat
     3241 ttgcgagcat gatcgcaatt atttcactgc cagggatatc aatggcttcc agtaaatcgc
     3301 tacagcaggc aattgccaac ataaaaatct ggcacaaagg tgaacagcgc gcgccgcata
     3361 agccattgtt attgctatac gtattagcgg gatacctgaa tggacatcca cgccttttcg
     3421 attatggatc ggaaatctac gaacctctgc acagtttact ggaacgtttt gggccacagc
     3481 gttcacatta ccgacctgat atgccgttct ggcgattaca aggcgacgga ttctggcaat
     3541 tacacaatgc cgagctttgc tcaacagcag ggagcagccg acaaccgcca gtgaaggaac
     3601 tgaacgaata ccacgttgca ggcggtttcg atgaacagca ctacgccctg gtaaccggca
     3661 ataaaaaact catcaatact ctggcgcaac agatactcga agcgcacttc accgagagta
     3721 ttcaggaaga aattgctgac gagctgggtt ttgatctcca gcaaatccgt aaacagcgcg
     3781 accctttatt tcgcaaaaat gtattacgcg cttataacta ccagtgcgcg atatgtggct
     3841 ttaatatgcg ccacgacgac accaccgtag cgttggaagc ggcgcatata aaatggaaac
     3901 agcaaggagg tccttgtgaa attcctaatg gcctggcgct ttgcgcaatt caccataaag
     3961 cctttgataa aggatcgatt ggcctggatg aaaatatgcg ggtactggta tcagatgcgg
     4021 taaacggcgg agggatcgtt gagagattat tctgggattt tgacgggaaa actattgcgt
     4081 tgccgcaggt gcgtaaaaat tacccttatg aggtgtttgt agagtggcat cggaaagagg
     4141 tttttagggg gtgaggttga taacagcatt caccctacaa ccttacattg tatttaaatt
     4201 agagctgagt taacagatag ttgtatatat aacaggcact tgtcacaacg attaactcaa
     4261 tatagttaga atacctcata ttaattaaaa aataagtaat gtcacaacag aggaaaagtc
     4321 acgatgactc ttgtagatag tgtcgaagca ggtaggctaa cgattagtga attgattgat
     4381 gccctggcga aagataaaaa ttacacagct tccagatggt atcagcgata ccgtgcattt
     4441 acgactttgc tacagcaaac ctcaactttt gctgagcctg caacagatgg tctggtcaaa
     4501 cagctttggt atgagcgtga caacggcatt gcaagtattc gccagggcgt tccatcctta
     4561 gcagaatatc agcaaagcct gccactgctt agagaactaa ctgaacgaat tcggcaacag
     4621 ccggatgaag aaacttacca atatgttggc aatgcacttc aacaagctaa agaaaacgga
     4681 cttctcaagc gtatgtattg gagtttgaga aatcgcgtct ttgccgcgtt ctcgccagaa
     4741 aactacacca gtactgtgga tgagaatgct tttaataaag cagcagaatt cttaaatcag
     4801 cacttccatc tcggtttggt actgaccgga aattggttac agaaaaacta tgaattgaaa
     4861 caagccatac acgcccaatc tcctgataca gatccttatt atgtgaatat ggccatctgg
     4921 catctctatg aattgctccg tgaacgcgat aatgaacaaa agcaggagaa agtagctagc
     4981 actacatcca taacccgcag tgagcccatc gagaacaaga tcatcctaca ttcaccaact
     5041 aacgtgatct tctttggccc ccctggcact ggcaagacct tcaggttgca gcaaaaaatg
     5101 aaagagtaca cttctcatgc tgttcccgct gatcgtgatg cctggctgga ttctcgcctt
     5161 gaatcgttga actggatgca ggttataacg ctggtgctgc tcgatcttgg gaaacgagcg
     5221 aaagttcgcc aaattattga acatatgtgg tttcaacgta aggcattatt aaacggtcgt
     5281 aatggcaatc tatcgaatac tgcctgggca gctttgcaat cctatacagt tcccgagtcg
     5341 ttaaccgttg attataagaa tcggcgtgag cctgccgtat ttaacaaaac agataacagc
     5401 gaatggtttc tagttgattc acagctcgag caagtggagg atttggtaga gctctacgcc
     5461 gaacttaaac gtggccctaa atctgccgaa gccatccagc gttttgcggt ggttacgttc
     5521 caccaatctt acggctatga agaatttatt gaaggtatac gcgctcgctc tgacgagagt
     5581 ggcaatatct cttatcccat tgagccgggt atctttatgc gcctttgcca acgtgcgaat
     5641 gccgatccag gacatcgcta cgccattttc attgatgaga tcaatcgcgg caacatatcc
     5701 aagatctttg gtgaactaat ctcactcatt gaagtagaca agcgtgcagg catgcccaat
     5761 gcgatgagcc tgcaactggc ttatagcggt gatcacttca gcgtacccgg caatgtcgat
     5821 atcatcggag ccatgaatac agcggaccgt tctttagctc tgatggacac ggctttgcgc
     5881 cgtcgctttg actttgtcga aatgatgcct gatctctctt tactgagtga agctaaggtg
     5941 aaaggcatag agctcgagtc gttgttagag aaactcaata gccgcatcga ggctctttac
     6001 gatcgtgaac atacgctggg gcatgcgttc tttatgccgg taaaaaatgc actcgatgcc
     6061 ggtgatgaag aagctgcgtt taaacaattg aagatcgcat tccagaaaaa gatcattccg
     6121 cttttacagg aatacttttt cgatgactgg aacaagatcc ggttggtgct ggcagacaat
     6181 caaaagcaag acgacaacct gcaattcgtg attgagaaaa ccgacgatct cgatacgctt
     6241 tttggtaaca accatggttt acgacgccat gatcagcaat caacagctta tgagctcaaa
     6301 gatttcgatc aagagatctg gaatattcca caggcttatc gttcaattta tcagccccaa
     6361 cagactcccc ttgatgagca ggcagtaaat catgggtgaa gttatctccg tttttgaata
     6421 tgacctcttg gggagtggaa aagcggcttc agttggcgca aaaccggtgc ctccacaggt
     6481 ctttaattat ttagaagcgc ttagcctggc aagtaatcaa gggagccagt tcctcaaact
     6541 cacctctcgc tcaggcttca aattgcttca ggttcaaaac tacgcaggga tgctttcaac
     6601 acctcacggt ttccagcttg agatactgcc caaggttggt aaaaacctca cagcggctaa
     6661 tgcacgtcaa acattgctga cgatgctaag ccatctgcct gggtttcgac atattcaaac
     6721 ccagcaagcc actcttcagg ctcagcgtat gcctttgctt gagattttta ttaaccagtt
     6781 tttgcatagc gtcagccaat tgcttaaaca gggtttacgc tccgactatg tgagcaagca
     6841 aggtaatctg gcttttatga agggcaagct gatgctttct gcacagctgc gacataacgc
     6901 ggtgaatcgc cataagtttt gtgtcgatta tgatgactac atgcctgatt gtgcagccaa
     6961 tcggctctta cactccgcac tagataagct tcttagtctg cagctgtcat cagataatca
     7021 acgctggctt tacgaactgc gttttgcgtt tgacggtatt ccacttagcc gggatattga
     7081 acgtgatata aacaacttac gcctcgagcg tggtatggct cattacaacg agccaatggc
     7141 atgggcgcaa ttgatcctga ggggaatgag cccgagtgct ttgcagggaa acaccaaagc
     7201 gatatcactt ttgttcccta tggaagcggt atttgaatcc tttgtggcac agaccctacc
     7261 cgacgagtta ccacctcacc tcaaagtttt acctcaagca gcaacttatt ctttggtaaa
     7321 gcatggactt aaagattgct ttaagcttcg ccccgacttg ctgattcaat cacacaaacc
     7381 agttcaaacc gaaatggtga tggatacaaa atggaagctg gtgaatagca gccagcagac
     7441 aaaatcactc tatggactgg cacaagctga cttctatcaa atgtttgcct acggacaaaa
     7501 atatcttggt gggaatggcg aaatgtatct gatttaccct gcgcatgatg actttagcca
     7561 acctatagcg cagcattttg ctttctcgga gactttaaaa ttatgggttg tgccataccg
     7621 gataatggca aaacgcggtg agaggatgat gtggccgaat gacgtgtcct gtacaatgag
     7681 ccccaaactg gatcggatgg ctgcttcagt atcctatagt ggaggcatga gatgacccat
     7741 gaataccagc gacaaagcct ggaacaacta ataactcagc aaacagcaac tcccagaagc
     7801 gccgccgttg tatttaatga gaaccaaacc ctctggcaaa cacttggctt tagccagtct
     7861 caagtttcgc tatggcttgc ctcattgcct caaagtaatc atctaaaagg gcccacaacg
     7921 gcaacgtacc aggttatacc tgatatcgcg gcacatttgg tatcattgtt gcatcaagct
     7981 ggtgggcgca tgccccttgc acaagtactg aaaaagctcc ctgctggtat tacgaccagt
     8041 gaacagcaga tacgcaaact ggcacagaag cacgcgcaga tagagattaa aggccccttg
     8101 ctggtactga taaattagcc ggcatttgta gtaaccattg gctgtattaa aggattttag
     8161 aaaacatcat ggcaaaagaa gcaaaaaaac aagacccgca acagcagcta gaagcactgt
     8221 tcaataaact ggtcaaacag cactccgagt ttactcagcc attggtcgaa ttaccgggaa
     8281 gtgcaggacg cttgcttaaa caggtccgtg atattgagca agtggcgccg ctattccgtg
     8341 atgctggctt agtgaaagcg gtccccaagc ccacaattgt taatttacgc gtagccggta
     8401 ttgcgactaa atcgtgctta gacaaggtat tgggtggttt tatttatgcc agtgcagcag
     8461 tgcgcacaga tttgttaata gatgacaata aagtcagcac ggtgggtagc gactcggtat
     8521 cagaactgga tgatattgat tttgaagctc agcgtaaacg gcttgattta attgaaattc
     8581 gtcaggctta tcagttagtt gagaaaaaac ttttaggtta tgagcctggc gatttaattt
     8641 tgctggatac gccgttgttt ttagcacggg atatggctcc gctggagaga aatgtccgtc
     8701 ataaacaaga gtacgaaaaa accagacaag tgatagaaac tttttggcaa aaccatcgct
     8761 ccagaatttt tccctggaat caaaatggtg tagtacttgc gagcattttg gctgagcgct
     8821 ttagtgccat tgtaagtatc gccaaacaag atttacgtac cgaacaagga cgcaaacaaa
     8881 ttttagcatc tgatgggttt tctaaagagt tgatgcaaaa tctggaagga ctggatgaaa
     8941 gtttggttgg cgtgggagat ttgcgattta tcaatggcat tttaggcaac tattcgcgca
     9001 ccatcgcatt tcgtttgtct gaacaagaaa agcgcattga acccaatatt gaagctaagc
     9061 aaggtctaat tggttttcac ttccgtagtg cccgaggtgg acagataaaa atggtgcaat
     9121 tagcgggtga cgaaccagaa tggaaaagtg ctgatctcga tttagttgcc tctcgcctga
     9181 tggtgctgga tatgcaaaac aaaggtaatg caatgccgtt gccacaatta cttggttatc
     9241 aacagcttgg tattttacct aaatttgcta cgttttatcg ccagggttta caccaggcac
     9301 taaagaataa tgatattgag gcagggtggt tggcaggcct cgatgaggga ttgaataatg
     9361 gcttataaag ttttaggtaa attagtcggt aacactggtg atgccagtca gttaactatg
     9421 gttgtacaag actcgttttc agtacgccgt ggtgagtttg tacgcattat gcaccaggaa
     9481 cgcaaagatg aggggcaagt cgccgtactg gggcgagtga ccaagataag ccgtactaat
     9541 atgctctata acgccggctt tggcgatggt gtaaccgagc ttgagctttt gcccggtgcg
     9601 aatgtaactg gtgagaatat gtttgctcag gtcgagctgg ttggttaccg cgatcctgtt
     9661 actcgtcaaa tcaaaattcc acgtcgcccg cttaacccgg gtaccgctgt tgaaaccgtc
     9721 gattttcagt ttttgagtga tttttacgaa tttaatgaac actccagttt gcacttaggt
     9781 aacttagtag gctacgacaa aggtgagaat acagttcctg tttttattga cgtgaacaag
     9841 ttggtcactg agcatttggc tgttctggca atgacgggtt caggtaagtc ttatacagtt
     9901 ggccgcatta ttgagcgttt agttgcggtt aataacggta cggtagtagt atttgaccca
     9961 catggtgaat acggtaaagc cctcgctggc ggtaatctgc aattctcaag cttcttagaa
    10021 gccactgacg ataagcgtga tcaagaagca ctgccactga taaaacagac ctttgaaaag
    10081 ctgcaagccg ccggtgcagg tattcaggtt tactcgccgc agcatgaatc ttttaaacat
    10141 aaatacgcca gtaagaatac gccattagcg cttcagtttg accattttga aatggacgat
    10201 attgctgaaa tattacctgg cttaacggaa ccgcagcaac gagtactcga tgttgcaatt
    10261 cgttattggc gctctgcaga taagactgag ccacgagata ttaaccgcct acgcttctta
    10321 ttaggtgatg gtattgaaga gttaaaagag tgggacgacc tttctgaagc tgaggcgaag
    10381 gcgttatcag gccgtagtgc cgccgtcgcc tcgatgaagc tttcacgtgt gcttaatgaa
    10441 gctaaaagtt tttacagcgc aacaatggct gagccaactg atatttacaa aatggtgggt
    10501 cgcccgagca atcaacaggg gcgtttagtc gttgttgact tacaaggttt gagtgatacc
    10561 gctaagcaag tcatttgtgc actgttatct agtgaaatat taaaagcagc ctcgagcaaa
    10621 acggacccgc tgcgcccatg ctttatcatt tacgaagagg gtcataattt tgccccggcc
    10681 ggcggcaacg cagtcagtca ccgcattatt aaaaagatcg ctggtgaagg tcgtaaattt
    10741 ggcgttggat ttggcattgt gagccagcgt ccatcgaaat tagattcaga tgtgacctct
    10801 cagtgcaata cgctaattac gatgcgctta aagaaccctg acgatcaacg atttatagct
    10861 aaagcatcag atatggtgag caaagctgat ctggaggaac tcccgagttt atcaaccggt
    10921 gaagctttgg tgtgtggtcg ttcaatccct gcaccactgc ttgttaaagt tggtagtaag
    10981 gcattaattc acggtggtga atcaccagag gtactgcgtg tttggggctc tttcagtggc
    11041 taaggtgcct atgcctgaag ttgcctgggt aaatgagaca tttccacttt tagcaagcgg
    11101 taagctattg gaccaggatg tattggtcca cagcctgggc tgtagcgttt ggaatacatt
    11161 cgggcatgaa cagtcattta tggcggtagt ggaatgcccg gctccaggga catttggtgc
    11221 tgatattcgc tctgactcgg gctggtttca taaatcttct gcatcgcctg tgtgtttaat
    11281 cgaatttgaa cgctttgatg gttcagcgaa ggggcagcaa aagctggaag aaaaattaaa
    11341 aaacttactg gaagcagcac agcgctggaa tcattgcccc aaaaccctgg tcctcagtgc
    11401 ctggagccaa ggcttagtag gtgtacctga tactcaaaaa ctgaaagaca tatgccgtat
    11461 gggttttact tcgtccacgg gtacccaagt aatcgccgct cctgatgttg aggtggtatt
    11521 tagtcgcttt ttatttatca aaaacctgaa catgattgtt ttggacagaa ttcactatga
    11581 ggtgttgatg tgaatataaa atcagcacat tttttgccag ggacgaataa acgccttggt
    11641 gacaaatacc tgttacttga atgccttggt gatggcagcc atggctgggt atggcgggcg
    11701 gaacgcctga aagatggaaa aattgtcgca gtaaaaatcc ctaaggatat tactcgggaa
    11761 gaccgtcagc ttgccgaagg taaagagctt ttaaacgtcg agccgcacga aaatattgtg
    11821 caaatttttg atatgggccg tatagataat gaatggttct atattgaaat ggagtatttt
    11881 cccagccaaa ccctggcgca aaagctcgat gacaggcagc gtaattttgg ccagacctat
    11941 gaacggttgt tcagaatata tcgtcaagtg ctctgtgctg tgcattattt ggccgaatta
    12001 ccagtaccaa tatctcatgg agatattaag ccacataaca ttttggttgg agagcgggac
    12061 ctggtgaagt tgacggactt tggcagctca gctttaccag aagaaattta cgttcgtacc
    12121 cgtgaaaatg gcgggactgt gctgtattcg gccccagagt tctccaacgt cgatagccgt
    12181 agaggcagcc tggaagagct actattgggc gacatctata gcttaggtgt tttgctatac
    12241 cagctgctga caggaaagct gccccatgat acgccagcac aagttcagcg ccacgcacct
    12301 ttcaagcttc cgacagagat taatagctct attcatacgg acctggaaca agtggttctt
    12361 acttgtttac aaaaacgagc ggaggatcgc ttttccacta tttccgactt aattcatgca
    12421 tttgattctg ctgccactaa gcaattaaaa gttggtgcta ttgctcccgt ttttcaaact
    12481 aaacctgctc aggactggtc aagtgcggta cttgaagcaa tgtccgatca atcttaccaa
    12541 aaagctgctc aattagcgtc gcaggagtat agccgcagta aagatctaca agctttacat
    12601 cagcaactta ttgccttgta tcgcgccaat cgcttattcg actttgaaaa agttgtagaa
    12661 gataataaag cgatcttact tgagggtaaa ttgactcaac cagcagcttt gttcgagttg
    12721 atagttaagg caaacctaca gttacgcaat atcagtcaag caaatttctg gctgttgcaa
    12781 cgaaagcaag ctgaagcaga aaacgcggcc accatgtatt tagaatcatc tatccttggc
    12841 cttgaagcga aatattctga agcaagagta ctattggaga aagtaaacca aacaactcca
    12901 atgaagttcc atgtgttaag ccaactcatt ttagtttgcg agcaaatgcg tgattactct
    12961 ggtgctgcgg cttatttgaa agcggcattg cgagtagcac cattggataa cagcatgaaa
    13021 gagaaaaaag aattgtatgc aaagcttgga gttatttgat tgaaaggatg accattgcgc
    13081 gaacaaggca tagtggatat ttaacacttc cgttcacata ccggacaaaa aaataaagtt
    13141 ttacataacg atttccttct tgattgagtt aagacaaaag cctctcccc
//
LOCUS       DAPHDX010000123        13182 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPHDX010000123 DAPHDX010000000
VERSION     DAPHDX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.124"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(21..1285)
                     /locus_tag="RH058_003540"
                     /pseudo
     CDS             complement(21..1285)
                     /locus_tag="RH058_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1487..1571)
                     /locus_tag="RH058_003541"
     tRNA            complement(1487..1571)
                     /locus_tag="RH058_003541"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1535..1537),aa:Leu,seq:caa)
     gene            1767..2786
                     /gene="ahr"
                     /locus_tag="RH058_003542"
     CDS             1767..2786
                     /gene="ahr"
                     /locus_tag="RH058_003542"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV3139075.1"
                     /translation="MSMIKCYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2916..4418
                     /locus_tag="RH058_003543"
     CDS             2916..4418
                     /locus_tag="RH058_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV3139076.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4537..5619)
                     /gene="lptG"
                     /locus_tag="RH058_003544"
     CDS             complement(4537..5619)
                     /gene="lptG"
                     /locus_tag="RH058_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV3139077.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5619..6719)
                     /gene="lptF"
                     /locus_tag="RH058_003545"
     CDS             complement(5619..6719)
                     /gene="lptF"
                     /locus_tag="RH058_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV3139078.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6986..8497
                     /gene="pepA"
                     /locus_tag="RH058_003546"
     CDS             6986..8497
                     /gene="pepA"
                     /locus_tag="RH058_003546"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV3139079.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8631..9074
                     /gene="holC"
                     /locus_tag="RH058_003547"
     CDS             8631..9074
                     /gene="holC"
                     /locus_tag="RH058_003547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV3139080.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9074..11929
                     /gene="valS"
                     /locus_tag="RH058_003548"
     CDS             9074..11929
                     /gene="valS"
                     /locus_tag="RH058_003548"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV3139081.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11984..13180)
                     /locus_tag="RH058_003549"
     CDS             complement(11984..13180)
                     /locus_tag="RH058_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDV3139082.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIIDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
BASE COUNT         3377 a         3414 c         3445 g         2946 t
ORIGIN      
        1 attcatatac ttagatagaa ttatcgaaaa gaactattaa tctttgcata gtcaaatgga
       61 gtgatagccg ccattctatt cgcatccaga taatcagccc accactgcag catcaacatt
      121 cgctccccaa gatgttccgc cttatggata taagccgccc gtactgagct acgttcctga
      181 tgactcatct gccgctctac tgcatccctc gaccacaatc ccgattcaat caaagaacta
      241 cacgccatcg tcctgaaacc atgtccacac acttccgttt tcgtgtcata gcccatcact
      301 cgcaaggcct tgttaagcgt attctcactc attggcttac gcggatcgtg atcaccaacg
      361 aagatcagct cacggtttcc actcatgctt ttgatttttt ccagaatggt gagagcttgt
      421 cgagataagg gtacaagatg tggagtccgc atcttcgaac cacgctgaga atgcttaact
      481 ccctccagtg gctcacgttc tcccggaatc gtccacatgg cagtatcaaa atcaacttct
      541 gaccagcgag caaaacgcaa ctcgcttgaa cggatgaaaa ctaacaatgt gagttcaacc
      601 gccagtcggg ttaacggcct gccagaatag tgatagatgc gttgaagcaa ttcaggaatg
      661 cggttaagtt ccagagcagc acgatgctgt cttttcgccg tagcaaccgc accagcaatc
      721 tcttgagcgg ggttgtagtc gattaatcca ctgtgtacag caaagcgtat aatcgcggta
      781 gtatgctgct gcaaacgagc agcgacttcg agacgcccag acgactccac cgctttgatg
      841 gggacaagca gatcccgagt cttcagatca gcaatgttcc gcttaccgat agcagcaaag
      901 agattatctt cgaggctttt caatacacga gcgctatgcg atgcagacca cttctgattg
      961 ctggcgtgcc actctctggc taccacttca aacgttatca cctcttgctc atgctctacc
     1021 ttaacagctt tcttgttctc actaggatcg acgccgttag ctaacaactt acgggcttca
     1081 tcccggcgac ctctggcatc gaccagcgag acttcagggt atttccccag cgccagcatc
     1141 ttctccttac cgccaaaacg ataacgaagt cgccagtatt tggagccatt agggtgacca
     1201 acaaaaccat gccttcaccg tcagtaagtt tataggcttt tgcttccggc ttagccgaac
     1261 ggaccttcac atcactcaga gccatgatga gtatcctttc aagggccctg tgtgggtaca
     1321 aacataatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt gatgtagatt
     1381 gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat actggatttc
     1441 aggcacaaaa aaagccgccc ttgagcgact cgatttgcat acgatgtggt gccgaaggcc
     1501 ggactcgaac cggcacgtat ttctacggtt gattttgaat caactgcgtc taccgatttc
     1561 gccacttcgg cactgaagag gtatgcggaa aacgttgtgg attatacctg gcgcacgcca
     1621 ccatgcaagc gccagcttgc tcccacttcg ctaagtgctg aaattttcag cattgcatac
     1681 agcgatgtgt aacctttgtc acactccagg caccccgccc tgccatgctc tacacttccc
     1741 agacaacacc agagaaggat caaaaaatgt cgatgataaa atgctatgcc gcaaaagaag
     1801 cgggcggcga actggaagtt tatgagtacg atcccggtga actgaagcca caagatgttg
     1861 aagtgcaggt ggattactgc gggatctgcc attccgatct gtcgatgatc gacaacgaat
     1921 ggggattttc acagtatccg ctggttgccg ggcatgaggt gattggtcgc gtggtggcgc
     1981 tcgggagcgc cgcgcaggat aaaggtttgc aggtcggtca gcgtgtcggg attggctgga
     2041 cggcgcgtag ctgcgggcat tgcgacgcct gtattagcgg taatcagatc aactgcgagc
     2101 aaggtgcggt gccaacaatt atgaatcgcg gtggctttgc cgagaagttg cgtgcagact
     2161 ggcaatgggt gattccactg ccagaaaata ttgatatcga gtccgccggg ccgctgctgt
     2221 gtggtggtat cacggtcttt aaaccactgt tgatgcacca tatcacagcc accagccgcg
     2281 ttggggtaat tggtattggc gggctggggc atatcgctat aaaacttctg cacgcaatgg
     2341 gatgcgaggt gacagccttt agttcgaatc cggcgaaaga gcaggaagta ctggcgatgg
     2401 gtgccgataa agtggtgaat agccgcgatc cgcaggcact gaaagcactg gcggggcagt
     2461 ttgatctcat tatcaacacc gtcaacgtca gcctcgactg gcagccttat tttgaggcgc
     2521 tgacctatgg cggtaatttc catacggtcg gtgcggttct cacgccgctg tctgttccgg
     2581 cctttacgtt aattgcgggc gaccgcagca tctctggttc tgctaccggc acgccttatg
     2641 agctgcgtaa gctgatgcgc tttgccgccc gcagcaaggt tgcaccgacc accgaattgt
     2701 tcccgatgtc gaaaattaac gacgccatca agcatgtgcg cgacggtaag gcgcgttacc
     2761 gcgtggttct gaaagccgac ttctgatccc tttgccgggt gcctgtcgcc cggcactaaa
     2821 atctctttgg aaggcatctc gcaatctgct gaatttagcg gtgaagagtg cgcagccgtt
     2881 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     2941 gcacgccaga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     3001 ctggcgcaac ggggacgggt aaaactgtca cgctgcaaaa actggcggag tctctatcgg
     3061 aaatcggtgt tccggtgttt atggccgatg tgaaaggcga tctgaccggc gtcgcgcagg
     3121 caggaacggc gtcggaaaaa ctgctcgcaa ggcttaaaaa tatcggcgtc aatgactggc
     3181 aaccgcatgc taatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     3241 gggcgacggt ttcggatctg gggccgctgt tgctggcgcg gctgttgaat ctcaacgatg
     3301 tgcaatctgg cgtgctgaat atcatcttcc gtattgctga cgatcaggga ttgttgctgc
     3361 tcgactttaa agatctgcgg gcgattaccc agtacatcgg cgataacgcc aaatctttcc
     3421 agaatcagta cggtaatatc agtagcgcat cggttggtgc catccagcgc ggactgttgt
     3481 cgctggaaca gcaaggcgca gcacacttct ttggcgagcc gatgctggat atcaaagact
     3541 ggatgcgcac cgataccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaagcttt
     3601 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttgtatgaac
     3661 aattgccgga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaggcac
     3721 atctgctgtt taacgatgca ccgcaggtac tgctggataa gattgagcag gtgatacggc
     3781 ttattcgctc aaaaggcgta ggcgtctggt tcgtttcgca aaacccgtct gatattccgg
     3841 ataatgtgct cgggcagctc ggtaatcgcg ttcaacacgc tttgcgtgct tttacgccca
     3901 aagatcagaa agcggtaaaa gctgcggcgc aaaccatgcg ggccaatccg gcatttgata
     3961 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt cttgatgtga
     4021 aaggaagtcc ttcagtggtg gaacgggcga tggtgattgc gccttgttcg cggatgggac
     4081 cggtgacgga agatgagcgt aatggcttga ttaatcactc tccggtgtat ggcaagtacg
     4141 aggatgaggt ggaccgggaa tccgcctatg agatgttgca aaaaggcgta caggcaagcg
     4201 ccgagcagca aaataatccc cccgcgaaag ggaaagaggt ggcggtggat gacggcattc
     4261 ttggtggatt aaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     4321 gtgtggtgca aacaatggcg aaaagcgccg cccgccaggt gacgaatcag attgtacgcg
     4381 ggatgttggg gagtttgctg gggggaagaa gaaggtaagt caggtgcggt ctgtgcccct
     4441 caccctaacc ctctccccaa aggggcgagg ggaccgatcg aactcgtatt tgactttatc
     4501 aacaatgtga aagggagctt tcgctccctt gcttggttac gattttctca ttaacagcca
     4561 caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg gcgggatgcc
     4621 ataaaccaac gtcaacgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     4681 gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agatgaacga
     4741 cagcgccatc agcatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     4801 gagctgataa cgtccagcgt cctgaccgct cgacttcaga tacttcacgt agttgtgcaa
     4861 accgctgatt gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     4921 gttggttttc caggtgccgc tcaccgtctg cgaaccggta atctgtttcg gattggtcag
     4981 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcagt
     5041 agcggcatag cgtacggatt gcagacgacg attctcgtta aaggcataaa tgctgatgcc
     5101 acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     5161 ccataagcct tgctgggtag agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     5221 acgcgccatc tgctcgccct gcggcgcgac ccattcgcca atcgccatcg tcagcaagac
     5281 cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa aaccagaagc
     5341 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     5401 cagagccgcc atcgggaaga agatttgcac atctttcggc acgctcagca aggtatacat
     5461 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaactt
     5521 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     5581 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     5641 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     5701 gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt acctttaccg
     5761 ccgttcgatt tcagggaggt ctggatcagg aagaacaaca gatacagcag catggctggc
     5821 agcatcgata gtacgcgccc ctgacgcggg ttaaccacgc tcagcggtac gaccataagt
     5881 gccatcataa acacggtgaa caccaacgtg atacgccagt tcagttctgc gcgagcacga
     5941 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
     6001 gccaccgcct ggtgaccaat gatcgcctga taatcctgga aatccgtaat gcggaaatca
     6061 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
     6121 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
     6181 tttggtcgaa tttgcgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
     6241 aacagcaccg aactgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
     6301 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccacggtccc
     6361 gcccacatca cgttaaccgc cgcgacgatt gccgtgaata ccgcaaggat cattgccgct
     6421 ttcaccagaa ccgctttgct caggccgcag gcatgcatta ccgtaatttc actttcggta
     6481 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
     6541 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
     6601 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
     6661 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
     6721 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
     6781 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
     6841 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
     6901 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
     6961 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
     7021 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
     7081 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc ggggcgaact
     7141 ggaaggaaaa ccggggcaga cattgttgct gcaccatgtt ccgaatgtac tttccgagcg
     7201 aattctcctt attggttgcg gcaaagaacg tgagctggat gagcgtcagt acaagcaggt
     7261 tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg tctgctttct
     7321 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
     7381 ggcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg aaccgcgtcg
     7441 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
     7501 cgcgatccag cacggtctgg cgattgccgc cgggattaaa gcagcaaaag atctcggcaa
     7561 tatgccgccg aatatctgta acgccgctta cctcgcctct caggcgcgcc aactggctga
     7621 cagctacagc aagaatgtca ttacccgcgt tatcggcgaa caacagatga aagagctggg
     7681 gatgcattcc tatctggcgg tcggtcaggg ttcgcagaac gaatcgctga tgtcggtgat
     7741 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
     7801 aaccttcgac tccggcggta tctcgatcaa gccttcagaa ggcatggatg agatgaagta
     7861 cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcgg agctacaact
     7921 gccgattaac gttatcggcg tgctggctgg ctgcgaaaac atgcctggcg ggcgtgccta
     7981 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
     8041 cgctgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgagcgtt ttgagccgga
     8101 agcggtgatt gacgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcacat
     8161 tactggtctg atggcgaacc acaatccgct ggcccatgaa ctgattgccg cgtctgaaca
     8221 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac aactggagtc
     8281 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
     8341 cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatatcg ccggtaccgc
     8401 ctggcgttct ggtaaagcga aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
     8461 cctgttaaac cgcgccgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
     8521 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
     8581 tagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccattt atgaaaaacg
     8641 cgacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcagc
     8701 tggtgtgtga aattgccgca gaacgttggc gcagtggtaa gcgcgtgctc atcgcctgtg
     8761 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
     8821 ttgttccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
     8881 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
     8941 cagattttgc caccgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
     9001 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
     9061 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
     9121 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
     9181 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
     9241 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctgtggca
     9301 ggtcggtact gaccacgcag ggatcgctac ccagatggtc gttgagcgca agattgccgc
     9361 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
     9421 atggaaagcg gaatccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactccgt
     9481 ggactgggag cgtgaacgct tcaccatgga cgaaggcctg tccaatgcgg tgaaagaagt
     9541 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga
     9601 tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
     9661 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagatg gtaaagatta
     9721 tctggtggtt gcgactactc gtccggaaac cctgctgggc gataccggcg tggccgttaa
     9781 cccggaagat ccgcgttaca aagatctgat tggcaaatat gtcattctgc cgctggttaa
     9841 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
     9901 gaagatcacc ccggcgcacg actttaacga ctatgaagtc ggtaaacgtc acgccctgcc
     9961 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agtgcccagg tgttcgatac
    10021 caaaggtaac gaatctgacg tttattccag cgagatcccc gcagaattcc agaaactgga
    10081 gcgttttgct gcacgtaaag cggtcgttgc tgcggttgac gcactcggcc tgctggaaga
    10141 aattaaacca catgacctga ccgttcctta tggcgaccgt ggtggcgtgg tcatcgaacc
    10201 gatgctgacc gaccagtggt acgtgcgtgc cgatgtcctg gcgaaaccgg cagttgaagc
    10261 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    10321 gatgcgcgat attcaggact ggtgtatctc tcgtcagttg tggtggggtc accgtatccc
    10381 ggcatggtat gacgaagcgg gtaacgttta tgttggccgc aacgaagacg aagtgcgtaa
    10441 agaaaataac ctcggtgctg atgttgtcct gcgtcaggac gaagacgttc tcgacacctg
    10501 gttctcttct gcgctgtgga ccttctctac ccttggctgg ccggaaaata ccgacgccct
    10561 gcgtcagttc cacccaacca gcgtgatggt atctggtttc gacatcatct tcttctggat
    10621 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    10681 gccgttccac actgtttaca tgaccggcct gattcgtgac gacgaaggcc agaagatgtc
    10741 caaatccaag ggtaacgtta ttgacccact ggatatggtt gacggtattt cgctgccaga
    10801 actgctggaa aaacgcaccg gcaatatgat gcagccacag ctggcggaca aaatccgtaa
    10861 gcgcaccgag aagcagttcc cgaacggtat tgaaccgcac ggtactgacg cgctgcgctt
    10921 caccctggcg gcgctggctt ctaccggtcg cgatatcaac tgggatatga aacgcctgga
    10981 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    11041 agaaggtcag gattgcggct tcaacggcgg cgaaatgacg ctgtcgctgg cggatcgctg
    11101 gattctggcg gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    11161 cttcgatatc gccgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    11221 tctggaactg actaagccgg taatgaacgg tggcaccgaa gccgaactgc gcggtactcg
    11281 ccatacgctg gtgaccgtgc tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    11341 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    11401 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    11461 cacagagtgg ctgaagcagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaacat
    11521 cgcgccgggc aaaccgctgg agctgctact gcgtggttgc agcgcggatg cagaacgtcg
    11581 cgtaaatgaa aaccgtggct tcctgcaaac gctggcgcgt ctggaaagca tcaccgtgct
    11641 gcctgccgat gacaaaggtc cggtttccgt tactaagatc atcgacggcg cagagctgct
    11701 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt
    11761 ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    11821 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgaa aagctggaag gctatgcaga
    11881 agcgaaagcg aagctgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag
    11941 taatgaaaac gaaggccgga gcatgctccg gcctttttat ctcttacaga aatggtaaag
    12001 aaggcataat tccccgtgaa atcctggcta agaagccttt atctggttgt tcttctttat
    12061 tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa
    12121 tcatccatat tttcagcagt ggataagcca gaccgcccgt aatcccggat atcaccacca
    12181 acgcacacat gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc
    12241 catcattcag tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg
    12301 tgctaacagc aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt
    12361 ccataaattg ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat
    12421 ttaatatttg atcgatgata atataaccag caacagcgag aaaaggtaat aaagctaaaa
    12481 atgcgagaat ggcatatttt atacaatacg tagtatctaa tttcaccttg aaacgatgta
    12541 taccgaaact ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa
    12601 aaataccaat ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac
    12661 ttgaactatt ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg
    12721 ccattaaaat cggcaagaaa aaggttaccc accagaaccc tttcatagag caattaaaac
    12781 taaatcttac gccgttgaga ctggtcatca aggcctgata acgcagtcct tttgctgcca
    12841 taaatataag caaaaccaac agtaacaaag tcaaaacaca gccaacaagt ggcatatctg
    12901 ctgacactgt cattaagatt gcgaaatagc aaaaaacaaa aacaagacaa ctaacaaaaa
    12961 cattcccacc agtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataga
    13021 gataacgctt acatttcatt aatgcccatg gcaaatagat acctagcgtt ataatcgtta
    13081 acaacacatt caccacacaa ataagaaaat atctctcacc tgtaccatga aaaacaaacg
    13141 aatgggacgg aacatccatt tcattaataa cttgagccat aa
//
LOCUS       DAPHDX010000124        13178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPHDX010000124 DAPHDX010000000
VERSION     DAPHDX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.125"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            140..1639
                     /gene="pitB"
                     /locus_tag="RH058_003550"
     CDS             140..1639
                     /gene="pitB"
                     /locus_tag="RH058_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV3139083.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1688..2380)
                     /locus_tag="RH058_003551"
     CDS             complement(1688..2380)
                     /locus_tag="RH058_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV3139084.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2554..3267
                     /locus_tag="RH058_003552"
     CDS             2554..3267
                     /locus_tag="RH058_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV3139085.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3299..4057
                     /locus_tag="RH058_003553"
     CDS             3299..4057
                     /locus_tag="RH058_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139086.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4103..5452
                     /locus_tag="RH058_003554"
     CDS             4103..5452
                     /locus_tag="RH058_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV3139087.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5452..6276
                     /locus_tag="RH058_003555"
     CDS             5452..6276
                     /locus_tag="RH058_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139088.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6288..6848
                     /locus_tag="RH058_003556"
     CDS             6288..6848
                     /locus_tag="RH058_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV3139089.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6879..7949
                     /gene="lptF"
                     /locus_tag="RH058_003557"
     CDS             6879..7949
                     /gene="lptF"
                     /locus_tag="RH058_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV3139090.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7946..9025
                     /gene="lptG"
                     /locus_tag="RH058_003558"
     CDS             7946..9025
                     /gene="lptG"
                     /locus_tag="RH058_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV3139091.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9061..10233)
                     /locus_tag="RH058_003559"
     CDS             complement(9061..10233)
                     /locus_tag="RH058_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3139092.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10233..10481)
                     /locus_tag="RH058_003560"
     CDS             complement(10233..10481)
                     /locus_tag="RH058_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3139093.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10513..11427)
                     /locus_tag="RH058_003561"
     CDS             complement(10513..11427)
                     /locus_tag="RH058_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV3139094.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11424..13154)
                     /locus_tag="RH058_003562"
     CDS             complement(11424..13154)
                     /locus_tag="RH058_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV3139095.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         3160 a         3272 c         3422 g         3324 t
ORIGIN      
        1 tttgaatgaa tgcttattgt ctgatacaca agaaataaca ctctttttat cgttaaaaaa
       61 tgatatttca ctttgcccat gccgttaaag tcactgataa tgcgtccgtt cgtaaattca
      121 aaatggcgta atctaatata tgctaaattt atttgttggc cttgatatat acacagggct
      181 tttgttattg cttgctctgg catttgtgtt gttctacgaa gcaatcaatg gttttcatga
      241 cacggcgaat gcggtggcaa ccgttattta tactcgtgcc atgcaaccac aacttgctgt
      301 ggtgatggcg gcatttttta acttttttgg cgtgttattg ggcggactta gcgttgccta
      361 tgccattgtc catatgttgc caaccgattt gttgctgaat atggggtcaa cccacggcct
      421 ggcgatggtc ttttccatgc tgctggcggc gattatctgg aacctgggaa cgtggttctt
      481 cggtttaccg gcctccagtt cgcacacctt gattggtgcg attatcggca tcggtttaac
      541 caacgcgctg ttaaccggct catcggtgat ggatgcgtta aacctgcgtg aagtgaccaa
      601 aattttctcc tcgctgattg tttcccctat cgtcggcctg gtcattgcgg gaggcctgat
      661 attcctgctg cgacgctact ggagcgggac gaaaaagcgt gaccgtattc accgcattcc
      721 ggaagatcgc aaaaagaaaa aaggcaaacg taaaccgcca ttctggacgc gtattgcgct
      781 gattgtttcc gctgcgggcg tggcgttttc gcacggcgcg aacgacggac aaaaagggat
      841 cggcctggtg atgctggtac tggtggggat tgcccctgct ggcttcgtcg tcaatatgaa
      901 cgcgtccggc tatgaaatta cccgtacccg cgatgccgtc accaacttcg aacactacct
      961 gcaacagcat cctgaactgc cgcagaagtt gattgcgatg gaacctccat tgcctgcagc
     1021 atcgactgat ggcacgcaag taacagagtt tcactgtcat ccggcaaata cctttgatgc
     1081 gattgcgcgc gttaaaacga tgctgccagg caatatggaa agttacgagc cgttaagcgt
     1141 gagtcagcgc agccagctgc gccgcattat gctgtgcatc tctgatacct ccgcgaagct
     1201 ggcgaaactg ccaggcgtca gtaaagaaga ccagaacctg ctgaaaaaac tgcgcagcga
     1261 tatgttaagc accattgagt acgctccggt gtggatcatc atggcggtag cactggcgct
     1321 cggcattggc accatgattg gctggcgtcg tgtagcgatg accatcggtg agaagattgg
     1381 taagcgcggc atgacgtatg cgcaaggcat ggcggcacaa atgacggcgg cagtgtctat
     1441 cggccttgcc agttatattg ggatgcctgt ctccacaaca cacgtcctct cgtctgcggt
     1501 agcagggacg atggtggtgg acggcggtgg gttacagcgt aaaacggtaa ccagcatcct
     1561 gatggcgtgg gtgtttactt taccggcggc aatttttctt tctggtgggc tgtactggat
     1621 agcattgcag ttgatttaac cttcctgaaa atgcccggtc ctggcgatcg ggcatttcca
     1681 tttttgacta gtgataacca cgcgcggtca taaaatccgt aatcgctttt tctgcatcaa
     1741 ccaacacctg ttccagtggc cgattggcat cgatatcaac cagttgtgca ccaccaaagg
     1801 ttaactgtgg cgttatggca atcttcctcg ccagcgattc ccgtttatgg tcgggtttac
     1861 gtgcacaggc aacttcaagg tcaacattga gtttgatgac cagatcaggc ttatggctcg
     1921 ccatccagtg aaacgcttta cgttcctggc ttgccagcca tgagacaaaa cgaccacctt
     1981 caacgttagg tgggaacacc gtaccatcgt aagcgccagg aatttggtcc tgaggataac
     2041 ggtcggttag aacaattaac ccgcgacgac gacaggcaag catatgacga aagcgcagta
     2101 agcgacgggc gacaaacgct gtaattacca gcgccggaac tggtccaggc aatttttttg
     2161 ctgttttcac ctgatttcgt tcaattgttt tatgtaagga ttttcccatc aacggtaatt
     2221 ttgtcactgc acgaccgaca tttccggcct gttttcctaa atgaactctt tcggcagcac
     2281 catatttttc gacaacggta atgagatgtt cacacaccgt tgacttgcct gaaccatcgc
     2341 taccaataac ggcaattaat ggaggtgtta ttgactgcat gaatatatcc ttaaatttaa
     2401 atatccatcc aaaatattta tttggttaat atgtttttat gaaagcgtaa ttcaggtcaa
     2461 tgtcacaatt aaccattgtc acaataaggt tgaacggata ttctgagcac ggaccacgta
     2521 atattcaata cattatttac tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt
     2581 aattccacgc cagtgcgtgt tattgccatt gtaggatgtg atggttcagg taaatcgacc
     2641 ctcacggcaa gcctggtaaa tgaactggca gcaagaatgc caacagaacg catttatctc
     2701 gggcaatcgt ccgggcgaat tggcgaatgg atttcacagc tccctgttat tggcgcacct
     2761 tttgggcgtt atctgcgaag taaagcggca catgtgcacg aaaagccctc aacaccgcct
     2821 ggcaatatta ctgcgctggt tatctatctg ctttcctgct ggcgggcgta caagtttcgc
     2881 aaaatgttgt gtaaaagcca gcaaggcttt ctgctcatca ccgaccgcta cccgcaggtt
     2941 gaagtgccgg ggtttcgctt tgatggcccg caattggcaa aaaccacggg cggtaacggt
     3001 tggataaaaa tgttaaggca gcgcgagctg aagctgtacc aatggatggc gtcttatttg
     3061 cccgtattgt tgattcgtct tggcattgat gaacaaaccg cgtttgcgcg taaacctgac
     3121 caccaactgg cagcgttaca ggaaaaaatc gccgttacgc cgcaactgac attcaatggc
     3181 gcaaagatcc ttgaactgga tggtcggcaa ccagccgatg aaattatgca agcgtcacta
     3241 cgcgcaattc acgccgcgct ttcctgatca ccaaaatgga taggggatga tttaacgaat
     3301 ggatgcacta caaacacaaa ctgttaatag tacaaccgca ccgcagccca actacattcc
     3361 ggggctgatt gcggtggtcg ggtgtgatgg caccggtaaa tccacgctga ccaccgacct
     3421 ggtgaaatcg ctgcaacaac actggcaaac cgagcgccgc tatttggggc tgctctccgg
     3481 cgaagacggc gacaaaatca aacgattgcc gttggttggc gtctggctgg aacggcgact
     3541 ggcggccaaa tcctcgaaaa cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc
     3601 ggtgattatg tactgcttct cgctgcgaag aatggcgaat ctgcgcaagg ttcagcgact
     3661 ggcgcaaagt ggcgttctgg tggtcagcga tcgcttcccg caggctgaaa tttcgggctt
     3721 ttattatgac ggaccgggga ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct
     3781 ggcgcagcgc gaacggcgtt tataccaaca aatggcgcaa tatcgcccgg aattaattat
     3841 tcgcctgggc attgatattg agactgccat ctcccgcaag cctgaccatg actatgccga
     3901 gctgcaggac aaaatcggcg tcatgtcgac gattggctat aacggcacaa aaattcttga
     3961 gattgattcc agagcgccct acagcgaagt gctggaacag gcacagaagg cggtttctct
     4021 ggttgccatc gtttctgacc gccgaagttt aacttaaggc tggcgggata ctttttacta
     4081 tcaacgataa ggtcaggctg aattggcggg ttttaacatc aaacattggt ttgcagatgg
     4141 cgcgtttcgc accattattc gcaatagcgc ctggttaggc tccagtaatg tcgtgagcgc
     4201 cttgctgggg ctgttggcgc tctcgtgtgc cggtaaaggg atgacgcccg ccatgtttgg
     4261 cgtactggtg attgtgcaat cgtacgccaa gtcgatcagc gattttatta agtttcagac
     4321 atggcagctg gtggttcagt acggaacgcc agccttgacc aacaataatc cgcagcaatt
     4381 tcgcaatgtc gtctcatttt ccttctcgct ggatatcgtc agcggcgcgg tggcgattgt
     4441 cggcggcatt gccttactgc cattcctttc ccattcatta ggtctggatg accaaagctt
     4501 ttggctggcg gcgctgtatt gcacgctcat tccttcaatg gcttcctcca cgccgaccgg
     4561 cattctgcgt gcggtagatc gcttcgattt aattgctgta cagcaggcga cgaaaccttt
     4621 tctgcgcgca gcggggagcg tcgtagcctg gtattttgac tttggttttg cgggttttgt
     4681 tattgcctgg tacgtgtcga atctggttgg cggcaccatg tactggtggt ttgcagcgcg
     4741 cgaattacgc cgcagaaata tccataatgc cttcaaattg aatctgtttg agtctgcccg
     4801 acacatcaaa ggcgcgtgga gttttgtctg gtcaaccaac attgcccact ccatctggtc
     4861 ggcgcgtaac tcgtgcagca cagtgctggt ggggatcgtg ttaggacccg ctgccgccgg
     4921 gttatttaaa atcgccatga cattcttcga cgccgccgga acgccagcgg gtttgctggg
     4981 taaaagtttt tacccagagg tgatgcgttt agatccgcgc accaccagac cgtggttgtt
     5041 aggtgtgaag tccggtttac tggcgggcgg aatcggtatt ctggtggcgc tcgcggtgtt
     5101 gatcgtcggc aagccgctca tttcgctggt gtttggcgtt aagtatctcg aagcgtatga
     5161 cctgattcag gtgatgctgg gcgcaattgt gatttcgatg ctgggcttcc ctcaggagtc
     5221 attgctattg atggcgggaa aacagcgcgc ttttctcgtg gcgcaaacca tcgcctcaat
     5281 cggctacatc gtattgctgt tcatgttctg tcatctgttt ggtgtgctgg gagcagcgtt
     5341 tgcttacttc ggtgggcaat gtctggacgt ggcgctctcg ttaattccaa ccttaaaggc
     5401 attttttcag cgccattcct tgctttataa cgcagccgga gagaaatcct gatgatgaga
     5461 aaatatttcc cgcttgaggc gagcgagcgt ctatttgttg cgattgaaga agatgatgtc
     5521 gttgatgcac aggttagttt gccgccgact attgcactaa gttgtaccac cgaaatcatt
     5581 catgataatt acgcgttatg cctgcaattc tggctcaatg gcgttaatcg tcaggagtta
     5641 ctgcgtttag ttcgcaaaca agccaaaggc gatgagctga ctgctgatga gcgcaaacaa
     5701 tttaaataca tgcgagcgcg ctacaagcac ctgcgttttg cgcaacggct gtatttaaag
     5761 aagcatcagg ccggattttt attcggcaaa acgaccgttt ttctggggcg ttttcaggac
     5821 ggctttcgta acggtaagaa aaatattgtg tcgtattacg gcaacctgtt acggatttat
     5881 ttaagctctc cggtctggtc gttggtgaac tattcattac gccatagcca gttagaaagc
     5941 gtaagcagtt ttattgccta tcggcaaaag caaatgcata ccctgaaaga gattattgcc
     6001 aaaccgcgac taaccggcag ggaatttcat gatgtgcgaa aaatcatcag ccaacaggtt
     6061 tcttattacg acaccttaag atcgcttgat cctgaaaata aagaggcgtt acagatttcc
     6121 cgtttcctcg ccgccattaa tggcctgatg ggagataaac acgacgacat ggtggcggat
     6181 gatatggaaa accgccagtc gtatgatgca ccgttggcgc tggatagcga tattcgtcag
     6241 cgcttagagc tgctgatttc gcgttttccg ctatagccgg agcaatgatg tcatctcgtt
     6301 tgattattgc gttgattatc atgctcttag ccccgggtgt gcaggcacac aatttcgtca
     6361 ctggcaaaac ggttacccct gtttatatcc aggaaggcgg cgagctgctt ctgaatagcg
     6421 acgacgaaat tcactaccaa aagtggaaca gcacacaatt agcaggaaaa gtccgcatta
     6481 ttcagtatat cgccgggcgt aagtcggcga agaaaaagaa ctcactgtta attaaagcgg
     6541 ttgaagcggc aaactttccg caggatcgtt ttcagcccac caccattgtg aacaccgatg
     6601 atgccatctt tggtactggc tattttgttg ttggcaaaat cgaaaaaaat aaacgccgct
     6661 atccgtgggc gcaattcatt attgacggca atggacaggg acgcgttgcc tggcgcttgc
     6721 cggagcagag ttcaaccatt ctggtgctga ataaagccgg gcaaattcag tgggcaaagg
     6781 acggcagcct gacgccggaa gaggtagatc atgtcattgc gctggcgcag aaactgatca
     6841 atgaataaaa aattcaaagg attgagggcg ttagtgagat gaagctggtt gaacactaca
     6901 tcatgcgtgg tacgcgccgt ctggtgctga ttatcgtcgg ttttctgatc tttattttcg
     6961 ccagctactc cgcacagcgt tatctgaccg aagcggcaaa tgggacatta gctcttgatg
     7021 ttgtgctgga tatcgttttc tacaaagtgc tgattgcact ggagatgttg ttacctgttg
     7081 gtctgtatgt gtcagttggc gtgacgctag ggcagatgta taccgactcg gaaattaccg
     7141 ctatctctgc ggcggggggc agtccgggac gcttgtacaa agccgttctt tatctggcga
     7201 taccgctaag tatttttgtc acccttctgt cgatgtatgg tcgtccgtgg gcttatacgc
     7261 agatttatca actggagcag cagtcgcagt cggagctgga tgttcgccag ttgcgggcaa
     7321 agaaatttaa cactaacgat aacggacgaa tgatcctttc gcagacggtt gatcaggata
     7381 ataatcgcct gactgacgcg ctgatttata cttctactgc caatcgaacc cgcattttcc
     7441 gcgcccgttc ggttgatgtg gttgacccat cacccgagaa accgaccgtt atgttgcaca
     7501 acggcactgc ctatcttctc gatcatcagg ggcgtgacga caacgaacag atctaccgta
     7561 atctgcaatt acatctgaat ccgctggatc aaagccctaa cgtcaaacgc aaagcaaaat
     7621 cggtcacgga gctggcgcgc tccgcctttc ctgccgatca tgccgaactg caatggcgac
     7681 aaagccgtgg cctgaccgca ttgttgatgg cgctgctggc catttcatta agtcgggtaa
     7741 aaccgcggca agggcgattt tcaacgttat tgccactgac gttgctgttt attgccattt
     7801 tttatggcgg cgacgtctgc cgtacgctgg tggctaacgg cgcgattcct ctcattcctg
     7861 gtttgtggtt agtgcccgga ctcatgctaa tgggcctgct gatgctggtc gcacgcgact
     7921 tctctttgct gcagaaattt tcccgatgaa tgttttcagt cgctatttaa tccgtcatct
     7981 ctttctcggt tttgccgccg ccgcagggct attgctgccg ctttttacca ccttcaacct
     8041 gattaacgaa ctggatgatg tcagcccggg cggttatcgc tggactcagg cggtgctggt
     8101 ggtgctaatg accttaccgc gcacactggt cgaactttcg ccgtttatcg ccttattggg
     8161 agggattgtt ggcctggggc agttatcgaa aaacagtgag cttaccgcca ttcgcagcac
     8221 ggggttttct atcttccgta ttgcactggt ggcgctggtt gccgggatat tgtggactgt
     8281 ttcgttaggc gcgatagatg agtgggtggc gtcgccattg cagcaacagg cgctgcaaat
     8341 caaatcgacc gccaccgcgt tgggggagga cgatgacatt accggcaata tgctgtgggc
     8401 caggcgcggc aatgaatttg tgacggtgaa atcgctgaat gaacagggcc agcctgtggg
     8461 cgtggagatt tttcattatc gtgacgatct ttcgctcgaa tcctacattt atgcacgcag
     8521 tgccaccatt aaagacgaca aaacgtggat cctgcatggt gtgaatcata aaaaatggct
     8581 taacggtaaa gaaacgctgg aaacatcaga taatcttgcc tggcaatcgg ccttcaccag
     8641 tatggatctt gatgagttat cgatgccggg gaatactttt tctgtccgtc agcttaatca
     8701 ttacatccat tatttgcagg aaaccgggca acccagcagc gaataccgcc ttgcactgtg
     8761 ggaaaaactg gggcaaccga tcctgaccct ggcgatgatt ttgctggctg tgccgtttac
     8821 ctttagcgcc ccgcgctcgc cagggatggg tagccgtctc gctgtaggcg tcatcgttgg
     8881 cttactcacc tggatcagct atcaaatcat ggtcaatttg ggattgctat ttgcgttaag
     8941 cgcacctgtt accgcgctcg gtttaccggt agcgtttgtg ttggtggcgt tgagcctggt
     9001 gtattggtat gacagacaac attaaacccg ccgtggcgca aacaccacgg cgggctcaca
     9061 ttactgcttc agagtagcaa atgcctgaat gatggcatcg atttgctcac gactgtgggc
     9121 agcatttacg ctacagcgca gcagcgtgat gcctgcaggc gcggcaggcg gcaggataag
     9181 attcacatac acgcccagcg agataagctc gcgccagatg cgtaaacctt cctctttcga
     9241 accgattatt actggcacta ccgggctaat atgtgaactt aactcataac ccagtttttg
     9301 cagaccgtca tacagatggt tggcgttatc catcagcttt tgccgcagct ctggttgtgt
     9361 ggcaatggtt cttaacgatg agcgcacggt cgcaatgcag gacggagaag gagaggcggt
     9421 aaaaatatac gggcggctgc cgtagcgcag cacttccatc gcttccgaac cgacagcaaa
     9481 accgccaatc gaggccagac ttttactgaa agtaccaacg ataatatcca catcatcttc
     9541 aacgccgaca gcttcagcca gtccgcgccc cgttgcgcct aacacgccaa acgaatgcgc
     9601 ttcatcgaca atcaaataac caccgagacg acgtttgata tcaacaattt ccgccagcgg
     9661 cgcaacgtca ccgagcatgc tgtagatgcc ctcaacgatg ataatcgcct ctttcgcccg
     9721 ttcaccgagg cgaaccatgc gccgttccag atctttagca tcgttatggc ggaaacgaat
     9781 aatttccgcg ccaccgagag aacaggcgtc ataaatactg gcgtggctgt cggcatccag
     9841 taacaccacg gcgttatggt cggcaagtgc gctgatgacg cctaaattag cggtataacc
     9901 ggttgaaaaa acgatagcgg tagggcgatt gaaaaactcg gcaatctctt tctctaacgc
     9961 caggtgcggc gcataactgc cgtttgccat acgagaaccg gtggtgccgg taccttgggc
    10021 cgctaacgcg gcctgacctt cgctgatggc atcatgatta aaggttaaac cgagataatt
    10081 attggtcccg gccagaatca cttttttatt gccgatccgg ccttcggtgg cggaatacac
    10141 ttcatcaata caggtgccaa acggtgttaa cccgttgtca ctaaattgca gtcgctcgcc
    10201 agcaaggcgc gcatatttat cgtatagccc cattatttat tctccagcca tggaagtaga
    10261 gtttccatta attgcgcagg ggttttgaca tccagcagaa tattaatagg aatggaaata
    10321 tcgaatctgt cttccagcat cattaagaga tccatcactt tgatcgactc caggccaaga
    10381 tcgttgacga gatcgctgtc gggcttaatt tccacaccgt tttcgaccaa atcctgtaaa
    10441 caggaaagga tgtaatccat tactatttca cgatttacca tagaaacgtt aacacacctt
    10501 taaacaagac aatcaaaata gcccgttgcg taatgcttgt tccaggctaa tcccgggaaa
    10561 ccaattaata tcttcagaaa tacggttatt acttgccgac cagtcggcgt gggttaattc
    10621 acgaattttc gagcgggtca gcataggttc tttacctgcc aggcgactca acgcggtact
    10681 gatatccgca aggcaggtga gtaccggcag aggaatacca accattcgca cggaaccaca
    10741 acgggcgtcg gcggcaagct gtcgtacgtg ttgccagtca tagccgccgg ggacgccatc
    10801 gcataattca taggtttgcg tctgtacagt ttcggcgctt aaccactgac ccactgcttg
    10861 cgcgaaatcg gtgacgtgca ggaaagagag ctgtgtatct ggcgcaccaa gtcgtggcag
    10921 caggccgcgc agcatccagt caaacagcgg ttttaactct ttatcgccgg gaccatacac
    10981 ggctgtcggg cgaaaaacgc ccagcgtaat ttcgtcagcc attgcagtca gacgttgttc
    11041 ggcgacgtgt ttggaatttg cgtaccagga gagctcggga tggcgcgccg ccagcgaaga
    11101 gataaacaga aaacgttggc aaaagccgct ctcttttgct gcttgcatca ggcgcaggct
    11161 gccgtcaacg ttacagtggg taaaaatctc ttctttgtgt ccgcgcactt gcccggcgca
    11221 atggaccacc gcgctggctc cggcaaccag ttcgctaagc gaatgtgtat cttccagcga
    11281 accgcgcacc caagtaagat tatcattgac gtgagcgcgg gcagtacgcg tcaatgcgcg
    11341 aacgtgaaag ccgcgggcga gcaggttatc gataatatat ttaccgataa acccggtagc
    11401 gcccgtcacc gcgacagttt gattcatgcc agggattcct gcacatgaag gcttgcagca
    11461 taagccttct gataacgttt tttcgcttcc gcacgggcag gcttgccgga ggacgttcgg
    11521 ggaatgctgt ggggcggcaa cagatcgata gccgctgtta cgccaaattc gctttggatc
    11581 cgtgctgcca gcgcgtggat aagctgtccg cgacgttctt catcgctgat ccgacactgg
    11641 atctgcaaaa tgattttttc ctgggcggta acaaaagcaa tcgcatcgcc agaatgaatt
    11701 tccggttcct gttccgctat atattcaata tcctgcggcc agatattacg gccacgaata
    11761 ataatcagat ctttaatgcg tccggtgaca tacagataac cgtccagcag ataaccgagg
    11821 tcgccggtgt ctaaccagcc cgtcgcggca atctcgtctt gcgaagcctg gtcgccaaag
    11881 taaccgctca tcagactggg gccggagatg caaatatggc ctaccacacg ttccgcgacc
    11941 ggcatacctg cttcattgcg gatttcaata ccatgttccg gcaacgcttt gccgcagttg
    12001 acgaaagtcg atacggcgcg tgtctctgca cccggcgcga cggctttacc ctgatattcg
    12061 aggatgtcgc gatccacttc gttaaccaca accccagagc cttcatcaga gaagctgaca
    12121 gccagcgcat tttctgccag tccgtagcac ggcatgaacg ttttatcgtc aaagttaacc
    12181 tgacggaaac attcagcgaa ttgatggagt tgttctgcgg agatcggctc agcaccaata
    12241 ccagcgacgc gccagcagga aagatccagt tcagcgagat ctttttcatt cacgcggcgt
    12301 tggcacaatt catagccaaa cggcggcgca acggaaacgg tgccgcgatt tttactgatc
    12361 aatttaagcc attgcaaagg acgcatggca aaatcctgag tgcgcaaata atctactgaa
    12421 agttgcgtgg cgacgggggt caggagaaag ccgaccagtc ccatatcatg gtagaaaggc
    12481 agccaggaga cgcagcggtc gccagggcgt aatttaatgc catcgtggct tatagcacgt
    12541 agattagcca tcacttcgcg atgggtgata atgacgccac ggggaaaacg ggtgctgccg
    12601 gaggtgtact ggaggtaggc gatatcgttc ggaactggac gctggagcgc aacatcggct
    12661 tccggtaatg ccttaaacca ggcgtggctt aaaacatgta attcggggtt gttatgcgtc
    12721 gcggcattga ccagtggcaa ccactcatca ccagtgataa tggctgcggg ctggcaactt
    12781 gccagtaaac cctggagttt ggcgctccag gaatcccgct gaccgacgcc cattggaatc
    12841 gccaacggga cggcgactaa gccagcatac tgacaggcaa aaaaagcctc tacaaacccg
    12901 ctactcgttt cggcaatcag cgccacgcgt tcgccttttt tcaggttcag cgataacaac
    12961 cgcttcgcac cagcttcggc acgcgttttt aacgtctgat attccagttg atcttcaagt
    13021 tggcaacgtc tgtcataaaa attcattccg gcgctactca gagcggcgta gtccaaagca
    13081 tcaaccagcg ttggaaaatc ggcatagcgc ataggtaggg aatgcgtaaa gattttatta
    13141 gacatataca ccacgaaaaa attggatatc aaaaataa
//
LOCUS       DAPHDX010000125        13129 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPHDX010000125 DAPHDX010000000
VERSION     DAPHDX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.126"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(177..845)
                     /gene="artM"
                     /locus_tag="RH058_003563"
     CDS             complement(177..845)
                     /gene="artM"
                     /locus_tag="RH058_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV3139096.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(845..1561)
                     /gene="artQ"
                     /locus_tag="RH058_003564"
     CDS             complement(845..1561)
                     /gene="artQ"
                     /locus_tag="RH058_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV3139097.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1568..2299)
                     /gene="artJ"
                     /locus_tag="RH058_003565"
     CDS             complement(1568..2299)
                     /gene="artJ"
                     /locus_tag="RH058_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3139098.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2317..3045)
                     /gene="artP"
                     /locus_tag="RH058_003566"
     CDS             complement(2317..3045)
                     /gene="artP"
                     /locus_tag="RH058_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV3139099.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDLALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3263..3778)
                     /locus_tag="RH058_003567"
     CDS             complement(3263..3778)
                     /locus_tag="RH058_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3139100.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3904..4227
                     /locus_tag="RH058_003568"
     CDS             3904..4227
                     /locus_tag="RH058_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV3139101.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGTDAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4224..5054
                     /gene="amiD"
                     /locus_tag="RH058_003569"
     CDS             4224..5054
                     /gene="amiD"
                     /locus_tag="RH058_003569"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV3139102.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5051..6064)
                     /locus_tag="RH058_003570"
     CDS             complement(5051..6064)
                     /locus_tag="RH058_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV3139103.1"
                     /translation="MKVLVTGATSGLGRNAIEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6163..7593)
                     /locus_tag="RH058_003571"
     CDS             complement(6163..7593)
                     /locus_tag="RH058_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV3139104.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7604..8605)
                     /gene="ltaE"
                     /locus_tag="RH058_003572"
     CDS             complement(7604..8605)
                     /gene="ltaE"
                     /locus_tag="RH058_003572"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV3139105.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYSCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8642..10360)
                     /gene="poxB"
                     /locus_tag="RH058_003573"
     CDS             complement(8642..10360)
                     /gene="poxB"
                     /locus_tag="RH058_003573"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV3139106.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPIVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10493..11461)
                     /gene="hcr"
                     /locus_tag="RH058_003574"
     CDS             complement(10493..11461)
                     /gene="hcr"
                     /locus_tag="RH058_003574"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV3139107.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11473..13125)
                     /gene="hcp"
                     /locus_tag="RH058_003575"
                     /gene_synonym="priS"
     CDS             complement(11473..13125)
                     /gene="hcp"
                     /locus_tag="RH058_003575"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV3139108.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
BASE COUNT         3073 a         3559 c         3488 g         3009 t
ORIGIN      
        1 aaaaaaagac ggatacctta gtgagttgtc cgtcttcatt ataagaattt atgcactatg
       61 taggccggat aaggcgtcca cgccgcatcc ggcacaggca ccgtgctgat gtctgatgcg
      121 acgctggcgc gtcttatcag acctacaaac ccccccggcg aatgtacgca gctacattaa
      181 tttcgccgtt cgaatgccag tgctttgcgc tcgatcagac gcatcatcag cgtcagcagg
      241 ccgttgacga ccaggtaaat aatccctgcc gcaccgaaca ccattacatc gtaggtgcgt
      301 ccgtacaaca actggctgta tcccatcact tccatcagcg taatggtgta agccagagag
      361 gtacttttga acaccagcac cacttcgttg gaataagaag agagcgagcg tttaaaggca
      421 tacggcagca ggatcgccag cgtatctttt ttgctcattc ccagggcgct acaggactgc
      481 cactgacctt ctgggatcgc acgaattgca ccgtaaaaca gctgcgtggt atacgccgca
      541 ctgttcagcg ataacgcaat cagcgcacat aaccacggtt ctgacaacaa atgccacagt
      601 gccggatact cctgcaaagt cggaaactgg cccggcccgt aataaatcag gaagatctgc
      661 accagcagcg gcgtaccggt aaacagcgtg atataacccc gcaccagcca caccagcacc
      721 ggcgttttca gcgtcaggat gatggtaaaa atcaatgcca gaatcagtgc cacaatcagc
      781 gaggcaacgg ttagcgtcag gctggtgtgt agccctttca tcagttcggg taaatactca
      841 aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag gtcaatgcgt ttgagaatgt
      901 actgactgag cagggtgatc accaggtaaa tcgccgccgc cacaatgtac caggtaaatg
      961 gttcctgggt acgagtagcg atgctttttg tttgcagcat taaatcattc acactaatca
     1021 aactgaccag cgcggtatct ttcagcagca ccagccactg gttaccgagg ccaggcagcg
     1081 catgacgcca catctgcggc atcaccagac ggaaaaagat agccgatttc gacagcccca
     1141 gcgcctgacc agactcccac tgacccaccg gcaccgcttt caacgcgccc cgaagcgttt
     1201 gcgaggcata ggcggcatac agcagtgaca gagcgatgac accacaaagg aacgggctaa
     1261 cgtcgaagtt ctcaatgtcc atctgcactg ggatctgcac gaacccaaga ttgatagtga
     1321 agccatccga aagcgtcagc agcagctgcg aggagccaaa atagataaac agcaccacca
     1381 gaatttctgg caggccacgc agaatggtta ccagcgctga acctgcccac gcgacaggac
     1441 gccattttgc cgactcccat accgcaaaga acatcgccag cgccagcccg acaatcaatg
     1501 cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc taaaggaaaa aattcattca
     1561 tcaggaatta cttctggaac catttgttgt agatggtttc gtaagtgcca tctttcttca
     1621 ctttttccag cgcagtgttg agtttctgct gcagctcagt gttgccctga cgtaccgcga
     1681 tgccgaggcc agtgccgaag taatctttat cggtcacttt gtcgcccacc gccgccagtt
     1741 tcgggttatc tttcagccac tcagtgacca ctgcggtgtc accgaagacg ccgtcgatac
     1801 gcccgttttg cagatccagt tttgcgttct ggtagctgtc atacggaacg gtagtgattt
     1861 ccgggtgctt atccataatg aatttctggt gtgtcgtccc gttctgtacg ccgacttttt
     1921 tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg acccacaaac agggcagagt
     1981 tgtcatagta cggggtggta aacagcacct gcttttcacg ctccggagtg atatccatgc
     2041 ccgccatcac ggcttctacg cgacggaatt tcaggcttgg gatcaggctg tcaaacgcct
     2101 ggttagagaa agtgcaggtt gcatcaatct ctttacacag cgcttgtgcc aggtcgacgt
     2161 caaaaccaac gatctggttg tttgcatcaa tcgattcaaa cggaggatag gatgcttcgg
     2221 tagcaaaacg aatggtttcg gcagctgtgg cggaaagact aaaacctgca attaacgcgg
     2281 caatcagaac ttttttcatt gttgttatcc cgaatcttag tgagagagat agtttttaaa
     2341 tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct tgttctacga tatgaccatt
     2401 ttccatatac accactcggc tggcggtttt acgcgccact tcaacttcgt gggtgacgat
     2461 cacctgggta atattcgttt ctgccagctc acgaatgatg ctgacgattt gtgccgtaat
     2521 ttccgggtcc agtgcggcgg tcggttcatc gaacagcagt acctgcggtt ccatcatcaa
     2581 cgcacgggca atagcaacac gctgctgctg accaccagaa agatgcagcg ggtaacgatc
     2641 gctataaggt ttgagacgca gacgttccag cagtttttct gcacgggcca gcgccagatc
     2701 tttactcaac cccagtacac ggcagggcgc ttcaatcagg ttttgctgca cggtcagatg
     2761 cggccacagg ttgtattgct gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc
     2821 tttgtcagag ggtgttttgg taaaatcgaa atggttgcct gcaatgttga gcgtaccgga
     2881 gcgcggcatc tcaagcagat tgagtacacg cagcagcgag cttttacccg cgccgctggg
     2941 gccaagtaac accagcgttt cgccctgtgg gcaatccagc gtgatatcga acagcgcctg
     3001 atgcgcgccg tagaagcaat taatgccgtt taattgaata ctcattgaca ctcgtatact
     3061 ggcagtctga tagctattga ggtcgaagat agtacctttg acagaataat tatgcaatat
     3121 ttctgcttta aaagttaaaa gcaaagcgca ttattcaata aacatagcac aaaataacgg
     3181 gggcggtggt cggtgagcat aaatgtcggc attcctcacg aaatgccgga caatttacgg
     3241 ggtttattgg ttgatcaagg cgttagcgat tctcgatgga ctgacggagc gtacccgccg
     3301 tggcatgaac gctgccgcct aaatagcgta catcgtcgat cacccaacac tggccttcct
     3361 gaatcattaa cacttcatcc tgccagctct gatcaccctg ttttagatcc acgcgcaatg
     3421 gaatgttacg ggcatcacga ttagggatag tcgatgcact ggcaacgtgg gcgctgtctg
     3481 gcaaggtggt tcgactggag aatggatcgt tggtcagtag ttcccgatgg ttattatccc
     3541 gggaggcatc gctaagcagt gtcgccagtt tgtcgctcag ataagggcgc aaggcggtga
     3601 tgtcgttgct gcggtgcaaa atgcggtagt cataaaattg ctgggccacg ttatccgggc
     3661 ctccttcaac gcaaggacca ctgcgtgtgc cgttatcttt ataagctgga gtgactgtgg
     3721 tgcaggcact gaggagcagt gcgcagggga taagcattgt caatttgctg tagcgcataa
     3781 tgatttcctt ataagcgatc gctctgaaag cgttctacga taataatgat atcctttcaa
     3841 taatagcgta tcagtccgat aatgcttttg agatcgaagg cttaacaaac aaggagatcg
     3901 atcatgcaat tttctacaac cccaactctg gaaggccaga ccatcgttga atattgcggt
     3961 gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc gtgatttctt tgctggtatc
     4021 cgcgatatcg ttggcggacg ctccggtgcg tatgaaaaag aactgcgcaa agcgcgggag
     4081 atcgcctttg aggaacttgg ctcccaggcc cgggcgctgg ggaccgatgc cgtcgtcggt
     4141 attgatatcg actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc
     4201 ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg gctgatcgct gccgctctgt
     4261 tactggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata
     4321 cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg
     4381 cagatgattt tgatagctcg ctggcgacac tgaccgataa gcaggtcagc tcgcattatc
     4441 tggtccctgc ggtaccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg
     4501 aacaagaact ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg
     4561 acacctctat tggcattgag ctggaaaacc gtggctggca aaaatcggcc ggagtgaaat
     4621 attttgcccc gtttgaaccg gcacagattc aggcgcttat tccactggcg aaagatatta
     4681 ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc
     4741 gcaaagacga tccggggcca ttatttccct ggcagcaact ggcgcagcag gggattggtg
     4801 cctggccgga tgcgcagcgg gttaactttt accttgccgg gcgcgcgccg cacacaccgg
     4861 tagataccgc gtcattgctg gagcttttgg cgcgctacgg ttatgacgtt aaacctgata
     4921 tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca gatgcatttc cgcccgacgt
     4981 tatataacgg cgaagcggat gcagaaactc aggcgattgc cgaagcattg ctggagaaat
     5041 acgggcagga ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca gttttctcga
     5101 taccttcatc cagggtgatg accggctgat aacctaactc ttcctgcgca cgcgtaatat
     5161 ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg ggtcagcggc ggctcttttg
     5221 ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat atccagcatc gggtagggga
     5281 cggaacgaat acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca
     5341 gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg
     5401 cttcctggct tgccagccac attgcgtgca cggcattttc atagtaggtc atatcgacca
     5461 gcgcactgcc gccatgcggt aacagaatac tgccgtagtg gtgcatcata tgcgccagac
     5521 ggggaataaa gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc
     5581 gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc ttcgctggct gctttgctgc
     5641 gggcaaactc gttggcgaag cggtgagggc gaaaatcttc tttaatatcg cgatggtggt
     5701 gataatcgaa gtacagggag ggggaagaga tatgaataaa gttacgcaca ccccaggcga
     5761 cagcccattc acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg
     5821 tcccccaggg tgaggtaaag ctggagcagt gccacagcgt atcaatgccc gcgagcatca
     5881 ctttagcttg tgatgaaacc agctcggtca gatccgccgg aacaaactct gcgcccattt
     5941 tttccagcaa tttgcccatt gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct
     6001 ggcataaaaa ctctatcgcg tttcgaccta agccgctggt ggcgccggta accagtacct
     6061 tcatatcaat ccactgttgt tgagaaaata acgtgcgcat tcttccgtga tttcccccat
     6121 gatgcaatgg gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc
     6181 tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat
     6241 caataaccag taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat
     6301 agtacgatag tcgcctttat cttccaggct aaaacacagt cgtcccagcc ccggcgcttt
     6361 catgccaaat aacaacgtaa gttgtttttc cggttcaacg acaatcactt tccagctatc
     6421 taccgcatcg ccagtctgta aatattcgcg ctccgggcgg cctttcgcca gcttatgacc
     6481 gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga aataacgctc
     6541 tttaccgccg atttggttca ctacctgcca taaagcagca aggctggcgg acgttttaac
     6601 ggtaaacccc gcctgtttgg caaaataacc gtactccggt cgccagcggg caaaggcctg
     6661 agcgtcgtag ccccagtcgc tggagttgac cagtttttcc tcctctttca acgtgctacg
     6721 taccgcgtca tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg cggtatcatc
     6781 cgccagcaga tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtacgga
     6841 agtaatcaca ttgagaaacc acaccgaaat ccagcgggtg gggaggggga tggggatcaa
     6901 ccagcggcgc ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac
     6961 ctctggtccg gcggcttcga agatgcggtg ttcgctggcc ggatgatcta acaacgccac
     7021 cagatagtgc agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg
     7081 cggcgttaac actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc
     7141 tgcgccaacg ataattccgg cccgaagttc ggtcacaggt acattcgctt cacgaagaat
     7201 gtccgccgta gcctgacgag cacgcagatg atccgactgc tcatgtggcg gggcctgcaa
     7261 cgaactgaga aagattaatt gcttaactgg tacttcacgt agcgcatcgc ggacgttgag
     7321 agccacctgg cgctcctgag cgataaaatc gccgccttcg cccatgctgt gcaccagaaa
     7381 atagacggta tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac
     7441 tttatggcaa ctgacgtttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc
     7501 cgccaggatc tgatgccctt gctggctgag tgtgcgcacc agatgctgac caatgtagcc
     7561 actggcaccg agaactaaaa tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg
     7621 cgcgccagtg ggcggcgact tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca
     7681 ggcggacaat cggcgaggcg ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg
     7741 cggcagcatt ttcttccccg acgcgaacaa acagcatatt agtgtcctga cgcatcacat
     7801 ccgcgcctgc ttcacgcagt tgctccgcca tccaggcggc gttgtcgtga tcttcctgca
     7861 accgtgcgac gttatttttc agggcataca tcccggcggc agccagaatc ccggactgac
     7921 gcatcccgcc accggccatt ttccgccagc ggatggcacg tttaatgtaa tcacgattac
     7981 cgacgagtaa tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat
     8041 cacaatattg cgtgatctct ttcagttcgc agctgtaagc caccacggca ttaaagatgc
     8101 gcgcaccgtc aacatgcagc gccagattgc gctcgcgggt aaattcccat gcttctttca
     8161 ggtattcacg cggcagcact ttgccgttgt gggtgttttc cagactgagt aatttggtgc
     8221 gggcgaaatg gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc
     8281 cgtcggcagc cgcgtcgatg ggttgcggct gaatactgcc cagcaccgct gcgccaccgg
     8341 cttcaaacag atagttatgc gcggcctgac cgacaatata ctcttcgcca cgttcgcagt
     8401 gactgagcag agcgaccagg ttggcctgag tgccggtagg cagaaaaatg gcggcttctt
     8461 taccggaaag ctctgctgca tagtcctgca gagcattaac ggtagggtcg tctccgtaaa
     8521 cgtcgtcccc aaccggggcg gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa
     8581 cggtatcact gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt
     8641 tttaccttag ccagttcgtt ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa
     8701 ttgcgcgcaa catatacagg ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa
     8761 tggctaactc ttctttggcg accaccacat ccaccaacac cggaccgtcg atagagaagg
     8821 cgcgttgcag ggcttcatca acttcagacg ctttttctac acggataccc gtaatgccgc
     8881 acgcttcggc aatgcgggca aagtttgtgt cgtgtagttc ggtgccgtca gtcaaatagc
     8941 caccagcttt catctccatc gccacaaagc ccagcacgct gttgttaaag acgacaattt
     9001 tcactggcag tttcatctgc actactgaga ggaaatcgcc catcaacatg ctaaaaccgc
     9061 catcgccgca catggcgacc acctgacgtt ctggctctgt cgcctgcgca cccagcgcct
     9121 gcggcatggc gttagccatc gaaccgtggt taaacgaacc taacaggcga cgcttgccgt
     9181 tcattttcag ataacgtgcc gcccataccg ttggcgtgcc gacgtcacag gtaaagatgg
     9241 cgtcatcggc ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct
     9301 cgctcggttt agctaaatcg tccagccctt tgcgggcatc gcggtaatct tccagcgctt
     9361 tatccagaaa cttgcgatcg gttttttctt ccaccagtgg cagcaatgcg cgcagggttg
     9421 atttgatatc gccgaccagc gccatatcta ccttgctatg cgcgccgatg ctggctggat
     9481 tgatatcaat ctgaataatt ttggcatcgg tcgggtagaa cgcacggtag gggaactgag
     9541 taccgagtag cactaacgtg tcggcgttca tcatggtatg gaaacctgac gagaagccga
     9601 ttaacccggt cattccaaca tcatacggat tatcgtattc gacatgttct ttaccgcgca
     9661 gggcatgaac aataggcgct ttaattttcc cggcaaactc aactaactct ttatgcgccc
     9721 ccgcgcagcc gctgccacac atcagggcga tattgctgga ataacgcagc agttgcgcca
     9781 gtttgcgtaa ctcttcttct tccggcgtca cgattggttg tggcgcatga taccagtgcg
     9841 tggttgcccc ttctggcgca ggttttaacg ccacgtcgcc tggtaacacg acaaccgaaa
     9901 cgccacggtt aagcaccgct ttgcgcatgg caatcgccag tacttgtgga atctgctccg
     9961 ggctggaaac cagctcgcaa tagtgactac attcgcggaa tagctcttgt gggtgggttt
    10021 cctggaaata gccgctgcca atttcgctgg agggaatatg agcggcaatc gccagtaccg
    10081 gaacgtgatt gcggtggcaa tcgaacaggc cgttgattaa gtgcaggttg ccggggccgc
    10141 acgatccggc acagaccgcc agttctccgc taagttgtgc ttcagcgcca gcggcaaagg
    10201 ccgccacttc ttcgtggcgg gtggacatcc actcgatggt gcccatgcga ttaagactgt
    10261 cactaagacc gttcagagag tcgcctgtga ctccccagat gcgtttcacc cctgccgatt
    10321 cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat ggttctccat ctcctgaatg
    10381 tgataacggt aacaagttta gttcatctga cggaggggga agggatggga gagaaaggag
    10441 gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg ggcataaggc gattatgcga
    10501 gaaccaaatc cccctgcgga tggcaggagc aggccagtac gtaaccttca gcgatttcgg
    10561 cgtcggtcag cgtcattgta ctgctcaccg tatattcacc ggaaaccact tttgtcttac
    10621 agcagccgca aacacccgca cggcaggcag cgacaaccgg aacgttattg ctttccagcg
    10681 cctccagtag cgtggtgcca accggggcgt aaaattctcg tgccggttgc agtttggtga
    10741 atttcagacc gctggtcgcc gcttctgcta ctggggtgaa gaatttctct ttaaagaaac
    10801 gcgtcacgcc gagcgctttc acttcctgct ctacccaatc catatacgga gccgggccgc
    10861 aggtcatcac ggtacgtgaa gctaagtcag gtacacctgc cagcagttcg cgagtgagac
    10921 gaccagcgat aaagccttcg gtaacgttat tttctgccac cagcgttacc ggatagttac
    10981 gccactcatc ggcgaaaata acatcctgcg gcgtacgcac gttgtagatc acccgcacat
    11041 cggcctgtgg acggttcttc gcaagccagc gacgcatcga cataatcggc gtgacgccgc
    11101 agcctgccgc cagcaacagg aatttatctt ctgctttatc gtcgcaggta aattccccca
    11161 tcgcgtccga aagccagaga taatcaccgc gttttacatc gcgcgtcagc cactgggagc
    11221 cgacaccgtc atcaatccgc cgcacggtca gggtgatata ttcactcacg cctggcgtgg
    11281 aggaaatggt gtaagcacgc agcgtttccg ctgagtttcg cacgctgacc agtgcatatt
    11341 gcccggcgcg atatgggtag taatcgtggc aaatcaggga aatcgtccac acatccggcg
    11401 tttcttgcgt aatgtgatga acctgcatcc gccacgggca ttgattcgtt ggcatcgtca
    11461 tcgacaaact ccttacgcgc tcaacagttg cttcatgtct tcttcaacag tggtgataga
    11521 acgcaggcca aatttctcgt tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc
    11581 agtcgggccg gtgacgatat ttttcacgcc cagagaaagc agcgtcagca gaatgacgat
    11641 cgctttctgt tcaaaccagg agagcaccag cgacagcggc agatcgttga caccgcagcc
    11701 cagtttctct gccagagtga cagccagaat aatcgctgag taagcatcgt tacattgacc
    11761 tgcatctacc agacgcggca gaccttcgat atcgccaaac tcaagtttgt taaagcgata
    11821 tttaccacag gcgagggtca ggatcaggca gtcatccggc acgctggtgg cgaaatcggt
    11881 gaagtagtgg cgctcgccgc gtgcgccgtc acagccacca agcaggaaga tatgacgcag
    11941 tttttcacgg ctcaccagat caatcagcgt atcagcagcg ccaagcagcg tctggcgacc
    12001 aaaacccacg gtgataaggt gcggaatttc gctgtacggg aagcctgcca tctgttgcgc
    12061 ctgggtgata accgcagaga aatcatcacc gtccagatga cgcacgccag gccagccaac
    12121 aatgctgcgg gtccagatac gatcgtcata agcgcctacg gttgggtcga tgatgcagtt
    12181 cgaggtcatc acgatggggc cagggaaacg agcgaactcc acttgctgat tctgccagcc
    12241 actgccgtag ttaccgacca gatgcttgaa tttacgcagc tccgggtagc catgcgcagg
    12301 cagcatttcg ccgtgggtgt agacattaac gcccgtgcct tcggtctgtt ccagcaggtt
    12361 gtagagatct ttgagatcgt gaccggaaat caggatgcat ttacccgccg tcgctttgac
    12421 gttgacctgg gttggcgtcg ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct
    12481 catcactttg aagttcatct ggccgatttc cattgaacac tcaagaagcg cgttcatatc
    12541 ggcaggccag gtccccagcc acgccatgat tttatgatac tgggcataaa tatcgttgtc
    12601 gtattgaccg agaacatgcg cgtgttccat ataggccgct gcacctttca ggccatacag
    12661 gcacagcaga cgcaggccga gaatgttttc gccaatcgcc gctttatctt tgttaggggt
    12721 aaattctgct gcctgacgtt gcagctcgcc gagatcgtcg ctcaccagtt gcaggtcagc
    12781 catcgggtta tcgacgcgcg cgttggcatc tacagccagg cattgcgctt tcagcgcctc
    12841 gcgcagggca atcgcttcac gagcgtagcc gacaatacgc ggagaatcga agttaacgtt
    12901 ggtcagggtt gagaaaaagg cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc
    12961 gtattcacgc gcttttaccg cccaggcaga aagcccttgc agcgccgcga tgagtaaatc
    13021 ctgaaggtca gaagtttccg ccgttttacc acacatcccc tgcgcgtatg agcagccgtt
    13081 tcctgccgga gtacggatag tttgttcaca ttgcacacaa aacatgatc
//
LOCUS       DAPHDX010000126        13071 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPHDX010000126 DAPHDX010000000
VERSION     DAPHDX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.127"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            403..1092
                     /gene="mutH"
                     /locus_tag="RH058_003576"
     CDS             403..1092
                     /gene="mutH"
                     /locus_tag="RH058_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV3139109.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1161..1874
                     /locus_tag="RH058_003577"
     CDS             1161..1874
                     /locus_tag="RH058_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV3139110.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2012..2230
                     /gene="ygdR"
                     /locus_tag="RH058_003578"
     CDS             2012..2230
                     /gene="ygdR"
                     /locus_tag="RH058_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV3139111.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2338..3378
                     /locus_tag="RH058_003579"
     CDS             2338..3378
                     /locus_tag="RH058_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV3139112.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="RH058_003580"
     CDS             complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="RH058_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV3139113.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4596..6755)
                     /gene="aas"
                     /locus_tag="RH058_003581"
     CDS             complement(4596..6755)
                     /gene="aas"
                     /locus_tag="RH058_003581"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV3139114.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVQEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7341..8372
                     /gene="galR"
                     /locus_tag="RH058_003582"
     CDS             7341..8372
                     /gene="galR"
                     /locus_tag="RH058_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV3139115.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="RH058_003583"
     CDS             complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="RH058_003583"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV3139116.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9763..10698
                     /gene="lysR"
                     /locus_tag="RH058_003584"
     CDS             9763..10698
                     /gene="lysR"
                     /locus_tag="RH058_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV3139117.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="RH058_003585"
     CDS             complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="RH058_003585"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV3139118.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLMEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11506..12924)
                     /gene="araE"
                     /locus_tag="RH058_003586"
     CDS             complement(11506..12924)
                     /gene="araE"
                     /locus_tag="RH058_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV3139119.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3326 a         3506 c         3206 g         3033 t
ORIGIN      
        1 gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt
       61 tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg
      121 aaccactaaa aactggagtt tcatcgcacg tcaaggctgt aaatggaaac agtagtggag
      181 gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc
      241 agtgaccaga tttggaaaac ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa
      301 aagcaatata aatcagggaa ttgaacaaca catgtggaaa aagttacact gcgaatattc
      361 ggcacataat tgctgtttgt tttttaatca aggtatcatg acatgtccca acctcgccca
      421 ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt
      481 tatacattgg gagaactggc ggcacttgcc gggcttgtta cgccagagaa tttaaaacgc
      541 gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa
      601 cctgagcaag attttgccgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt
      661 ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt taacgggtaa tagcggggtg
      721 acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg tactgtggat accggttgaa
      781 ggcgagcgca gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg
      841 aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga tatgattgtt
      901 ctcggtcagg ttgagcggat caccgctcgt cacggggaat atttacagat aagaccgaaa
      961 gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg gattctgacg
     1021 ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt
     1081 ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt
     1141 tttttggcag ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg
     1201 cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt
     1261 ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg
     1321 cgggagccat ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga
     1381 caaatccgct ttttacaata ttcagtcagg aaatttctgc ccgtgatttg attctgcttc
     1441 tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg
     1501 aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc
     1561 tgctggatat tatctttagc ctcgactcgg tgattacggc tgtgggtctg tcagatcacc
     1621 tgtttattat gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct
     1681 cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc tctttcctga
     1741 ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca
     1801 tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacttgatt cgcaacaaaa
     1861 agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tccttttacc
     1921 ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata aacagactat
     1981 tatcataggt aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc
     2041 agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga
     2101 tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag
     2161 ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat
     2221 tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt
     2281 cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg
     2341 caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg
     2401 acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct
     2461 cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg
     2521 caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag
     2581 ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg
     2641 gatcaggcgc tggaccggaa gaatatccgc gaagcgctgc atgacagcct caagcgccta
     2701 cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccgac caactgcttc
     2761 ggcaaactcg gttatagctg gacggattct gcgcctgcgg tttcgctgct ggatacgctg
     2821 gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa
     2881 actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt
     2941 gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa
     3001 gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg
     3061 accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg
     3121 ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc
     3181 agacgtcatg gcctggaccc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt
     3241 gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt
     3301 ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat
     3361 acttatccgg caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
     3421 gctgccagat ccacagcgcc gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa
     3481 tgggcacgac cgggatgcct atcattaccg ccagcgagta aatgcccagc atcaacaaca
     3541 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
     3601 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc atcacgccaa
     3661 tcagcatcaa caaggcatag gctggcagca gctcgtgttg cagggaaaaa atcagtacca
     3721 ccacgccaat caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaactaact
     3781 tcgctgccgc acctgcgcca accacgatac caatcgctac catcgcgttg agataggtgg
     3841 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
     3901 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
     3961 tgcgccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
     4021 actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac
     4081 cgtaggccag tgcgcatgcg gccagggcga cgaggacatg ccagtcagcc agcacaccac
     4141 cggctacgga accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt
     4201 tcactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg
     4261 ctgcagcacc aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac
     4321 tggctgcgcc cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc
     4381 tatccgccac ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt
     4441 gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa
     4501 acagtagggc attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc
     4561 ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg
     4621 tctacccagc ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt
     4681 ttcagatagc gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac
     4741 tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct
     4801 ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc
     4861 agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg
     4921 cggccctgaa tctgcacaaa gcccagctcg tcaaaacgca caatatcgcc agtgtcatac
     4981 cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttcctg tacacctggc
     5041 ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc
     5101 tcttcgatac cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc
     5161 gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcaca
     5221 ccgtagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct
     5281 tgtagttttt ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg
     5341 aaacgcgcgt agtgaccgag gaaagtcgaa gtgccgaaca acacggtgca actgcggtca
     5401 tacaccaact ccggcacaat acggtaatgc agcgggcttg gataaagaaa cacttctgca
     5461 cctgtgagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac
     5521 gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc
     5581 gccagaatgc ttttatggct atggacgacg cctttcggat ggccttcaga accggaggta
     5641 aaaaggatca gcgcctcttc ttccggctgc tgtttaacct gtgccagacg cggcatcagc
     5701 aaatgagcga agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga
     5761 tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga
     5821 aactggcggg aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct
     5881 tttaccccgg cagtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa
     5941 atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca
     6001 ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag
     6061 tctggggtaa agttgacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgca
     6121 ctcagtaaag attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt
     6181 atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc
     6241 atctccacct gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta
     6301 accagacctt tcaggcggct gaagtgcgtg agttccgccc cttcaatacg cacaggaata
     6361 accgttgcac cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag
     6421 cctgtagtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga
     6481 cgtaccagat gtttaatagc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat
     6541 gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga
     6601 cgcacaggta aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta
     6661 attagaacgc gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa
     6721 acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag
     6781 taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg
     6841 cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac
     6901 cgaataatct caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt
     6961 cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt
     7021 tactgttaca gattgatgac cggccaaaaa aaacctgcgc atctgcgcag gctggtgtaa
     7081 ttcatgtgct caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag
     7141 caacctgaag ccaaacgcca ccagcggtca atcgcaacag catttcgcaa agtgtaacca
     7201 aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg
     7261 aaaacacgcc accccttgaa ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt
     7321 ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt
     7381 cagtcgccac cgtttcccgc gtcattaata attcacctaa agccagcgaa gcttcccggc
     7441 tggctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc
     7501 tggcgcagca gaccactgaa acggtcggtc tgatcgttgg tgatgtttcc gatccgtttt
     7561 tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga
     7621 ttggcaacgg ttaccacaac gaacaaaaag agcgtcaggc tattgagcaa ctgatccgcc
     7681 atcgctgtgc tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat
     7741 taatgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc
     7801 gttgtattgc tcttgacgat cgttacggtg cctggctggc aacgcgtcat ttaattcagc
     7861 aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc
     7921 gtctgcaagg gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg
     7981 tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac
     8041 gaggaagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg
     8101 gcgttctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg
     8161 atgtgctggt gtcacgctat gtgcgcccgc gcctgaccac cgtgcgttac ccaatcgtga
     8221 cgatggcgac ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg
     8281 aaattactaa tgtctttagt ccgacgctgg tacgtcgcca ttcagtgtca actccgtcgc
     8341 tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt
     8401 aattcttcga tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact
     8461 tctggtaaca gcggacggct gttgtagttg gatgacattg atgcgccata tgcccctgta
     8521 tcatgcagta ccagataatc acccgccttc acttccggca aggcgcgggt ttcaacattt
     8581 cccccttcct gctgggtaaa gacatcgccc gattcacata atggcccggc gacgacggtt
     8641 tccaccgttg gcgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg
     8701 taactaccgt acattgccgg gcgcatcaga tcgttgaacc cagcatcaac cagcacaaag
     8761 tggcggctac ccatttgttt gacgctccgc acctgagtaa ttaatacgcc agactgcgct
     8821 accaggaagc gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgggcgatt
     8881 tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc
     8941 tgttgataag gaacagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat
     9001 tcgatgacct gacgcaccat agcaccacac acctgttcca gatgggcata atcaacgcca
     9061 gaaccaatgt gcatgtgaat gccgaccagc tgcagatgat gacgttgtat cacgtccagt
     9121 gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt
     9181 ttttggctat gtccgtgacc aaaccccgga ttaacgcgca gccatacccg atgccctggc
     9241 gaaacctggc ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat
     9301 tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg
     9361 gggtgcgttt gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg
     9421 gaatccactt tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct
     9481 ttctgtgcaa agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt
     9541 tgcgcatcgt agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg
     9601 gcggtgagat cggtatcggt gctgaacagt gaatgtggca taacaaactc cagataagtg
     9661 cttttttatg attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct
     9721 atgcaaaaat gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta
     9781 cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac
     9841 ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc
     9901 gggctaaaat tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt
     9961 ctgtttgaag aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa
    10021 agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa
    10081 tcttttttac cgcagctcct gcaacccttt ctggcacgtt atcccgatgt cagcttaaat
    10141 atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta
    10201 ggactcactg aaacgctcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta
    10261 gatgaagtgt gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt attaacgccg
    10321 gatgattttc agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg
    10381 ctggatcagc tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc
    10441 gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc
    10501 accgcactgg attatgcggc aagtggttta gtggtgcggc ggttcagtat tgcggttccg
    10561 ttcaccgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt
    10621 agtgagcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg
    10681 tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca
    10741 caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga
    10801 cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg
    10861 cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt
    10921 catccgcttc aggaataaga caattgatgg aaaattgttc catcagccgc ccgcgataaa
    10981 aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg
    11041 taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa
    11101 tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc
    11161 gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac
    11221 gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa
    11281 gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt
    11341 cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac
    11401 cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta
    11461 ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt
    11521 tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt
    11581 ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca
    11641 gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat
    11701 attcgacacc cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg
    11761 ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat
    11821 acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc
    11881 aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat
    11941 tttcagagcc ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa
    12001 cataaaggtc agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc
    12061 cgccattttg aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg
    12121 catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa
    12181 cagtgcccaa ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg
    12241 cgctttttcc gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc
    12301 cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac
    12361 tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt
    12421 atcggataaa aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat
    12481 cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc
    12541 gatcccgaca gcaatgccca gcaccacccg agcggcgatt aacatctcta cgctggtcgc
    12601 aaaagcggac cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt
    12661 acgacccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat
    12721 catgctacta accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa
    12781 cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc
    12841 agctaccgaa acaaacatat tcatacgccg cgtatcccgc aaagaacgtg gcgttaaagc
    12901 agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa
    12961 aatacgtgaa caactcacgc aggtgtcagg tcggaaacag cataaatatg gattaaattg
    13021 ctacgacatg tcgttatgtg atggatattc caattttcaa attaagttga a
//
LOCUS       DAPHDX010000127        12954 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPHDX010000127 DAPHDX010000000
VERSION     DAPHDX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.128"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            146..1255
                     /gene="livK"
                     /locus_tag="RH058_003587"
     CDS             146..1255
                     /gene="livK"
                     /locus_tag="RH058_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV3139120.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1303..2229
                     /gene="livH"
                     /locus_tag="RH058_003588"
     CDS             1303..2229
                     /gene="livH"
                     /locus_tag="RH058_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV3139121.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIAGAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2226..3503
                     /gene="livM"
                     /locus_tag="RH058_003589"
     CDS             2226..3503
                     /gene="livM"
                     /locus_tag="RH058_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV3139122.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3500..4267
                     /gene="livG"
                     /locus_tag="RH058_003590"
     CDS             3500..4267
                     /gene="livG"
                     /locus_tag="RH058_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV3139123.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4269..4982
                     /gene="livF"
                     /locus_tag="RH058_003591"
     CDS             4269..4982
                     /gene="livF"
                     /locus_tag="RH058_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV3139124.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5378..6694
                     /gene="ugpB"
                     /locus_tag="RH058_003592"
     CDS             5378..6694
                     /gene="ugpB"
                     /locus_tag="RH058_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV3139125.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6792..7679
                     /gene="ugpA"
                     /locus_tag="RH058_003593"
     CDS             6792..7679
                     /gene="ugpA"
                     /locus_tag="RH058_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV3139126.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7676..8521
                     /gene="ugpE"
                     /locus_tag="RH058_003594"
     CDS             7676..8521
                     /gene="ugpE"
                     /locus_tag="RH058_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV3139127.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8523..9593
                     /gene="ugpC"
                     /locus_tag="RH058_003595"
     CDS             8523..9593
                     /gene="ugpC"
                     /locus_tag="RH058_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV3139128.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9590..10333
                     /gene="ugpQ"
                     /locus_tag="RH058_003596"
     CDS             9590..10333
                     /gene="ugpQ"
                     /locus_tag="RH058_003596"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV3139129.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10320..10760)
                     /locus_tag="RH058_003597"
     CDS             complement(10320..10760)
                     /locus_tag="RH058_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV3139130.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10880..12622
                     /gene="ggt"
                     /locus_tag="RH058_003598"
     CDS             10880..12622
                     /gene="ggt"
                     /locus_tag="RH058_003598"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV3139131.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12660..12944)
                     /locus_tag="RH058_003599"
     CDS             complement(12660..12944)
                     /locus_tag="RH058_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDV3139132.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2862 a         3325 c         3726 g         3041 t
ORIGIN      
        1 gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag acaggcaaaa
       61 acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca aagcaacaca
      121 acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat
      181 gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg ccgttgtcgg
      241 cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc
      301 gattaaagac attaatgcca aagggggaat taaaggcgat aagctggtgg gcgtggaata
      361 tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaacgacgg
      421 cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat cagatatcta
      481 cgaagacgaa ggtattctga tgatctcgcc gggagcgacc aacccggagc tgacccaacg
      541 cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc caacggcggc
      601 aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg acaaacaaca
      661 gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta acgccaacgt
      721 cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga tcgcccgcct
      781 gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa tggggcagat
      841 gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg aaggtgtggg
      901 taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg tcactatgcc
      961 aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga aagcagacaa
     1021 gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat ctctggcgac
     1081 tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt taaaagctaa
     1141 cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt
     1201 tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca agtgatcatc
     1261 ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga gcagtttttg
     1321 tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc gctgatagcc
     1381 atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca cggcgaggtt
     1441 tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat gatgggcatt
     1501 gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat tgccggcgcc
     1561 tacggctgga gtatcgaacg ggtggcttac cgcccggtgc gtaattctaa gcgcctgatt
     1621 gcgcttattt ccgcaatcgg tatgtccatc ttcctgcaaa actacgtcag cctgaccgaa
     1681 ggttcgcgcg atgtggcgct gcccagcctg tttaacggtc agtgggtggt gggccatagc
     1741 gaaaacttct ctgcctctat taccaccatg caggcggtga tctggattgt caccttcctc
     1801 gccatgctgg cgctgacgat tttcattcgc tattcccgta tggggcgcgc ctgtcgtgcc
     1861 tgcgcggaag atctgaaaat ggcgagtctg cttggcatta acaccgaccg ggtgattgcg
     1921 ctgacctttg tgattggcgc ggcgatggcg gcggtggcgg gtgtgctgct cggtcagttc
     1981 tacggcgtaa ttaaccccta catcggcttt atggccggga tgaaagcctt taccgctgcg
     2041 gtgctcggtg ggattggcag cattccaggg gcgatgattg gtgggctgat cctggggatt
     2101 gcggaggcgc tctcttctgc ctatctgagt acggaatata aggatgtggt ttcatttgcc
     2161 ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc ggaggtagag
     2221 aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg ttttttgtcc
     2281 tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg gtggtcgaca
     2341 gcgcttcgga tgtccgttgg cagtgggtgt ttatcggcac ggcggtggtc tttttcttcc
     2401 agcttttgcg accggctttc cagaaagggc taaaaagcgt ttccggaccg aagtttattc
     2461 tgcccgcgat tgatggctcc acggtgaagc agaaactatt cctcgtggcg ctgctggtgc
     2521 ttgcggtggc gtggccgttt atggtgtcgc gtgggacggt ggatatcgcc accctgacca
     2581 tgatctacat tatcctcggc ctcgggctga acgtggttgt tggtctttct ggtctgctgg
     2641 tgctggggta cggcggcttt tacgccatcg gcgcttacac ttttgcgctg ctcaatcact
     2701 attacggcct gggcttctgg acctgtctgc cgattgccgg gttaatggca gcggcggccg
     2761 gcttcctgct cggcttcccg gtgcttcgct tgcgcggcga ctatctggcg atcgttaccc
     2821 tcggtttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt accggcggcc
     2881 cgaacggaat cagtcagatc ccgaaaccga cactcttcgg actcgagttc agccgtaccg
     2941 ctcgtgaagg cggctgggac acgttcagta atttctttgg cctgaaatac gatccctccg
     3001 atcgtgtcat cttcctctac ctggtggcgt tgctgctggt ggtgctaagc ctgtttgtca
     3061 ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt gaagacgaga
     3121 tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgactgcc tttaccataa
     3181 gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc tttgtcagcc
     3241 cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg ctcggcggta
     3301 tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtatcg cgcgaattga
     3361 tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg ctgatgatga
     3421 tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg aaaaacggcg
     3481 cagcgaaagg agagcaggca tgagtcagcc attattatct gttaacggtc tgatgatgcg
     3541 cttcggcggc ttgctggcgg tgaacaacgt caatcttgaa ctgtacccgc aggagatcgt
     3601 ctcgttaatc ggccctaacg gtgccggaaa aaccacggtt tttaactgcc tgaccggatt
     3661 ctacaaaccc accggcggca ctattttact gcgtgatcag catctggaag gtttaccggg
     3721 gcagcaaatt gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc tgttccgtga
     3781 aatgacggtg attgaaaacc tgctggtggc gcagcatcaa caactgaaaa ccgggctgtt
     3841 ctctggcctg ttgaaaacgc cttctttccg tcgcgcccag agcgaagcgc tcgaccgcgc
     3901 cgccacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg cgagtaacct
     3961 ggcctatggt gaccaacgcc gtctggagat tgcccgctgc atggtgacgc agccggagat
     4021 tttaatgctc gacgaaccgg cggcaggtct taacccaaaa gaaaccaaag agctggatga
     4081 gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg aacacgatat
     4141 gaagctggtg atggggattt cagaccgaat ttatgtggtc aatcagggga cgccactggc
     4201 aaacggtacg ccggagcaga tccgtaataa cccggacgtg atccgtgcct atttaggtga
     4261 ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa
     4321 tccaggcgct gcatgaggtg agcctgcata tcaatcaggg cgaaattgtc acccttattg
     4381 gcgcgaatgg cgcggggaaa accaccttgc tcggcacatt atgcggcgac ccgcgcgcca
     4441 ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg gcgaaaatca
     4501 tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg atgacggtgg
     4561 aagagaacct ggcgatgggc ggcttctttg ccgaacgtga ccagtttcag gagcgcataa
     4621 agtgggtgta tgagctgttt ccacgtcttc atgagcgtcg tgttcagcgg gcgggcacca
     4681 tgtccggcgg tgaacagcag atgctggcga ttggtcgcgc gctgatgagc aacccgcgtt
     4741 tgttgttgct tgatgagcca tcgctcggtc ttgcgccgat tatcatccag caaattttcg
     4801 acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag cagaacgcca
     4861 accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat gtggtgctct
     4921 ccgataccgg cgacgcgctg ctggcgaacg aagcagtgag aagtgcgtat ttaggcgggt
     4981 aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga caaatgtaaa
     5041 attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata ttgaatttgc
     5101 acgatcttgt aggccggata aggcgtttac gccgcatctg gcatgaaacg atgagtaatc
     5161 tgtagagttt gattcagacc ttctattttc cgcttatccg tgccccatct cccattttcc
     5221 ctcacccacg ccgtcaccgc cttgtcatct ttctgacacc ttactatctt acaaatgtaa
     5281 caaaaaagtt atttttctgt aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg
     5341 tgaattcgcg cattcggtac aacaagagag ataaacgatg aaaccgttac attatacagc
     5401 ttcagcactg gcgctcggac tggcgttaat ggggaatgca caggcagtga cgaccattcc
     5461 gttctggcat tctatggaag gggaactggg taaagaggtg gattctctgg cccaacgttt
     5521 taacgccgaa aatccggatt acaaaattgt accgacctat aaaggcaact acgaacagaa
     5581 tttaagcgcg gggattgccg catttcgtac cggcaacgct ccggctattt tgcaggttta
     5641 tgaagttggc accgccacca tgatggcatc gaaagccatt aaaccggtat atgacgtgtt
     5701 taaagaggcg gggattcaat tcgatgagtc gcagtttgtg ccgacggttt ccggttacta
     5761 ctccgacagc aaaacgggcc acttactctc ccagccattc aacagctcga cccccgttct
     5821 ctattacaac aaagacgcct tcaagaaagc aggattagac ccggaacaac cgccgaaaac
     5881 ctggcaggat ctggcggact atgccgcgaa actgaaagcc tccggtatga agtgcggcta
     5941 cgccagcggc tggcagggct ggatccaact ggaaaacttt agcgcctgga acggtctgcc
     6001 gtttgccagc aaaaacaacg gctttgacgg cacggacgcg gtgctggagt tcaacaagcc
     6061 ggagcaggtg aaacacatcg ccatgctcga ggagatgaac aagaagggcg acttcagcta
     6121 cgtcggtcgt aaggatgaat ccaccgagaa gttctataac ggtgattgcg cgatgaccac
     6181 cgcctcttcc ggttctctcg ctaatattcg tgagtacgcc aaatttaact atggcgtagg
     6241 catgatgcct tacgacgccg atgcgaaaga tgcaccgcaa aacgccatta tcggcggggc
     6301 cagcctgtgg gtgatgcagg gcaaaaataa agaaacgtat accggcgtgg cgaagttcct
     6361 cgacttcctc gcgaagccag aaaacgctgc cgagtggcat cagaaaaccg gttatctgcc
     6421 aatcaccaaa gcagcgtatg acctgacccg tgagcagggc ttttacgaga aaaacccagg
     6481 ggcggatacc gcgacgcgtc agatgctgaa taagccgccg ttgccgttca ccaaagggct
     6541 gcgtctgggc aacatgccgc agatccgcgt gattgtggat gaagagctgg agagcgtgtg
     6601 gaccggtaag aagacaccac agcaggcact ggataccgcc gttgagcgtg gaaatcagtt
     6661 gctgcgccgc tttgagaaat cgacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc
     6721 ttatctgacc tggcatcgcg tgtaggccgg ataagcgaag cgcatccggc acagttcagg
     6781 aattaaccgt aatgtcatca tcccgtccgg tgttccgctc gcgctggctg ccttatctgc
     6841 tggtcgcgcc gcagctcatc atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt
     6901 tgtggtactc gctacaaagc gtcgatccgt ttggtttctc cagccagttt gtcggcctgg
     6961 ataacttcgt cacgctgttc catgacagct actatctcga ctccttctgg acgacgataa
     7021 aattcagcac ctttgtcacc gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg
     7081 tggagtacat cgtgcgcggc agccgtttct atcaaacctt aatgttgctg ccttacgccg
     7141 tggctcccgc cgttgccgcc gtattgtgga tcttcctgtt taaccccggt cgcgggctga
     7201 tcacccattt tctcgcggag ttcggctacg actggaacca cgcgcaaaac agcggtcagg
     7261 caatgtttct ggtggtgttt gcctcagtat ggaagcaaat cagctacaac ttcctgttct
     7321 tctatgccgc gctgcaatcc attccccgtt cgttgatcga agccgcagcc atcgacggtg
     7381 cagggccgat tcgccgcttc tttaagattg cgctgccgct tatcgccccg gtgagtttct
     7441 tcctgctggt agtgaacctg gtgtatgcct tcttcgacac cttcccggtg atcgacgccg
     7501 ccacgtccgg cgggccggtt caggccacca cgacgctgat ttataagatc taccgcgaag
     7561 gttttaccgg actggatctg gcttcgtctg ccgcacagtc ggtggtgttg atgttcctcg
     7621 tcatcgtgct gacggtggtg cagttccgct atgttgaaag caaggtgcgt taccaatgat
     7681 tgagaaccgt ccgtggctga cgatattcag ccataccatg ctgatcctcg ggatcgcggt
     7741 gatcctcttc ccgctgtacg tggcgtttgt cgcggcgacg ctggataaac aggccgtcta
     7801 tgccgcgccg atgacgctca tccccggcac acatctgctg gaaaacatcc acaacatctg
     7861 ggtgaacggg gtaggcacga atagcgcgcc gttctggcgg atgttgctta acagctttgt
     7921 gatggcgttc agcattacgc tcggcaaaat taccgtctcg atgctctcgg catttgccat
     7981 tgtctggttt cgttttccgc tacgtaacct cttcttctgg atgattttta tcaccctgat
     8041 gctgccggtt gaagtacgta tcttcccgac ggtggaagtc atcgccaacc tgcagatgct
     8101 cgacagctac gccggtttaa cgctgccgct gatggcctcg gcgaccgcta ctttcctgtt
     8161 ccgccagttc tttatgacgc tgccggatga gctggtggaa gccgcgcgga tcgacggcgc
     8221 atcgccaatg cgcttctttt gcgacatcgt ttttccgctc tccaaaacta atctggcggc
     8281 gctgtttgtg atcaccttta tctacggctg gaatcagtat ttgtggccgt tgttgattat
     8341 taccgatgtg gatctcggca ccaccgtggc agggatcaaa gggatgatcg ctacaggcga
     8401 aggcaccacg gaatggaact cagtgatggc ggcgatgttg ttaacgctta tccctccggt
     8461 ggtgattgtt ttagtgatgc agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata
     8521 agatggcagg actgaaatta caggcagtaa ccaaaagctg ggatggtaaa acccaggtca
     8581 ttaaaccgct gacccttgat gtggcggatg gcgaatttat cgtgatggtc gggccgtctg
     8641 gctgcgggaa atcgacgctg ctgcgcatgg ttgccgggct ggagcgggtg acagaaggcg
     8701 atatctggat caacgaccag cgcgtgactg aaatggagcc aaaagatcgc gggattgcga
     8761 tggtgttcca gaactacgcg ctttatccgc atatgagtgt cgaagaaaac atggcgtggg
     8821 ggctgaaaat tcgcggcatg ggcaagcagc aaattgccga gcgcgttaaa gaagcggcgc
     8881 gcattctgga gctggacggt ctgctcaaac gtcgcccgcg cgagctttcc ggcggtcagc
     8941 gccagcgtgt ggcgatgggc cgcgcgattg tgcgcgatcc ggcggtgttc ctgtttgatg
     9001 agccgctctc taacctcgat gccaagctgc gcgtgcagat gcgtcttgaa ctgcaacagt
     9061 tgcaccgtcg cctgaaaacg acttcactct acgttactca cgatcaggtt gaagcgatga
     9121 cgctcgccca gcgagtaatg gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag
     9181 ttgaagtcta cgaaaagccc gccagcctgt ttgtagcgag ttttatcggc agtccggcga
     9241 tgaacctgct gacaggccgc gtgaataccg aaggcacgca tttcgaactg gacggcggta
     9301 tcgtgctacc gctaaacggt ggctaccgtc agtatgccgg gcgtaaaatg actctcggca
     9361 ttcgcccgga acatattgcg ctaagctcgc aagcagaagg tggcgtaccg ctggtgatgg
     9421 atacgctgga gatcctcggc gcagataacc tggcgcacgg acgctggggc gagcagaagc
     9481 tggtggtgcg actggcgcat caggagcgcc cgacggcagg cagcacgctg tggctgcatc
     9541 tggcggaaaa tcagctgcat ctttttgatg gtgaaacagg acaacgagta tgagtaactg
     9601 gccttatccc cgcatcgtcg ctcatcgtgg cggcggtaag ctggcccccg aaaacaccct
     9661 ggcggcaatc gacgttgggg caaaatacgg tcataagatg atcgaatttg acgcgaagtt
     9721 atcgaaagat ggcgagatct tcctgctcca tgacgacaat ctcgaacgca ccagcaacgg
     9781 ctggggcgtc gcgggtgaac tgaactggca ggatttactg cgcgtggatg cgggcagttg
     9841 gtacagcaaa gcgtttaaag gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg
     9901 tcgcgaacac gggatgatgg cgaatatcga aatcaaaccc accactggca ctggaccatt
     9961 aacgggcaaa atggtggcgc tggcggcacg ccaactgtgg gccggtatga cgccgccgct
    10021 gctgtcatcg tttgagattg atgctttaga agctgcacag ctggcggcac cggaactgcc
    10081 gcgtggttta ttgcttgatg agtggcgcga cgactggcgc gaactgaccg cgcggctggg
    10141 ctgcgtctct attcatctca atcataagtt actcgataaa gtgcgagtga tgcagttgaa
    10201 agacgccgga ctgcggattc tggtttatac cgtcaataaa ccccagcgcg cggcagagtt
    10261 gctgcgctgg ggcgtggatt gcatctgtac cgatgcgatt gacgtgattg gtccgaactt
    10321 tacggcccaa tagttttcaa cggaatgtca ggctgcggcg tgctgggctg attcagcatg
    10381 tcgccgtttc tcctctccgg cagcatatgc tgctgattca acgaactatc cggattacgg
    10441 ttgctgttta acatcccgcc gttggtgttg ggcagcattt gctgccgggc gggatttcgc
    10501 tcccccggct gtgactgcaa cacccgctga gaattattgt ttatctggtt ttctaaatgc
    10561 tgctgttgca actgagtttg cgttttcagt tgctgattca gcatcccttt ttgctggatt
    10621 tgctgagtct gcatctgggt ttgcatccgc tgctggctgg gtatctgata ccccggctgg
    10681 ttagggttgt tcagagtatt aatgggctgt gcaaagccga caaacggcag gagtgccgta
    10741 agaatcagaa gtcgtttcat cgcgtatcct cctctgaaga tatcctttaa gtttactcgc
    10801 ttcccgacaa aacgatgatt aattcagagt tatataccag gcttagctgg ggttgcccct
    10861 taatctctgg agaataacga tgataaaacc gacgttttta cgccgggtgg ccattgctgc
    10921 tctgctctca ggaagttgtt ttagcgccgc cgccgcgcct cctgcgccgc ccgtctcgta
    10981 tggtgtggag gaagatgtct tccacccggt acgcgcgaaa cagggaatgg tagcgtctgt
    11041 ggacgccact gccactcagg tgggggtgga tattctcaag gagggcggga atgccgttga
    11101 tgccgccgtg gcggtgggct acgcgctggc ggtaacgcat ccgcaggcag ggaatctggg
    11161 cggtggtggt tttatgttaa tccgctcgaa aaatggcaat accacggcta tcgatttccg
    11221 cgaaatggca cccgccaaag cgacccgcga tatgttcctc gatgatcagg gcaacccgga
    11281 cagcaaaaaa tcactcactt cgcatctggc ttccggcaca ccgggtacgg tagcaggttt
    11341 ctcgctggcg ctggataaat acggcaccat gccgctgaac aaagtcgtgc agcccgcgtt
    11401 taaactggca cgcgatggtt ttatcgttaa cgacgcgctg gctgacgatc tcaaaaccta
    11461 cggtagcgaa gtgttgccga atcacgaaaa cagtaaagct atcttctgga aagagggcga
    11521 gccgctgaaa aagggcgaca cgctggtgca ggcgaacctg gcaaagagcc tggagatgat
    11581 tgctgaaaac ggcccggacg aattctataa aggcacgatt gcggaacaga tcgcccagga
    11641 gatgcagaaa aacggtggct tgatcactaa agaagattta gcagcctata aagcggtcga
    11701 acgcactccg ataagcggcg attatcgcgg gtatcaggtt tactccatgc caccgccatc
    11761 ctccggcggg atccatatcg tacaaatcct caatattctg gaaaacttcg atatgaagaa
    11821 atacggcttt ggcagcgccg atgcgatgca aatcatggca gaagcggaga aatacgccta
    11881 cgccgaccgc tcggaatatc ttggcgaccc ggattttgtc aaagtaccgt ggcaggcgct
    11941 gaccaataaa gcctatgcca aatctattgc cgatcaaatt gatatcaata aagcgaagcc
    12001 atccagcgaa attcgccccg gcaagcttgc gccttatgag agtaatcaaa ctacccatta
    12061 ctcagtggtg gataaagatg gtaacgcggt ggcggtgacc tatacgctga acaccacctt
    12121 cggtacgggc attgtcgcgg gcgagagcgg tattctgctt aataaccaga tggatgattt
    12181 ctccgccaaa ccgggcgtac cgaacgttta cgggctggtg ggcggtgatg ccaacgccgt
    12241 cgggccgaac aaacgcccgc tgtcgtcgat gtcgccgacc attgtggtga aagacggtaa
    12301 aacctggctg gttaccggta gcccaggcgg tagccggatc atcactacag tgctgcaaat
    12361 ggtggtgaat agcatcgatt atggcatgaa cgtcgccgaa gcgaccaatg cgccgcgttt
    12421 ccaccatcag tggttgccgg acgagctgcg tgtcgaaaaa gggtttagcc cggatacgct
    12481 caagctgctg gaagcaaaag gtcagaaagt ggcgctgaaa gaggcgatgg gcagtacaca
    12541 aagcattatg gttgggccgg acggtgagtt gtacggcgca tccgacccgc gctcggtgga
    12601 tgatttaacg gcggggtact aaggttagcg gccctcttcg tgggaagagg gctattttgt
    12661 cagggcaagc cgaaggtagc cttttttatt tcgtaatcct gtagatattc ttccagcggc
    12721 tttgccgttc ccagagaatg aatttcacca ctgtctttat caataataaa aggtgcgtta
    12781 ccagctaagc gcgcggcctc atctccagtt tcgagaaatt ctcgtgcttc gaaacagaaa
    12841 taccagccct ggctaaagcg tccatgtaga gtaatgacga ccgggagatc tgcatcatca
    12901 aggtaatggt tcgctttcgc gaatgcgtcg tgataagtaa tcataattat aata
//
LOCUS       DAPHDX010000128        12935 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPHDX010000128 DAPHDX010000000
VERSION     DAPHDX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.129"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(565..1656)
                     /gene="ychF"
                     /locus_tag="RH058_003600"
     CDS             complement(565..1656)
                     /gene="ychF"
                     /locus_tag="RH058_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV3139133.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1773..2357)
                     /gene="pth"
                     /locus_tag="RH058_003601"
     CDS             complement(1773..2357)
                     /gene="pth"
                     /locus_tag="RH058_003601"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV3139134.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2635..2913
                     /gene="ychH"
                     /locus_tag="RH058_003602"
     CDS             2635..2913
                     /gene="ychH"
                     /locus_tag="RH058_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV3139135.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="RH058_003603"
     CDS             complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="RH058_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV3139136.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4772..5719)
                     /gene="prs"
                     /locus_tag="RH058_003604"
     CDS             complement(4772..5719)
                     /gene="prs"
                     /locus_tag="RH058_003604"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV3139137.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="RH058_003605"
     CDS             complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="RH058_003605"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV3139138.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="RH058_003606"
     CDS             complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="RH058_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV3139139.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7558..8814
                     /gene="hemA"
                     /locus_tag="RH058_003607"
     CDS             7558..8814
                     /gene="hemA"
                     /locus_tag="RH058_003607"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV3139140.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8856..9938
                     /gene="prfA"
                     /locus_tag="RH058_003608"
     CDS             8856..9938
                     /gene="prfA"
                     /locus_tag="RH058_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV3139141.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9938..10771
                     /gene="prmC"
                     /locus_tag="RH058_003609"
     CDS             9938..10771
                     /gene="prmC"
                     /locus_tag="RH058_003609"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV3139142.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10768..11160
                     /locus_tag="RH058_003610"
     CDS             10768..11160
                     /locus_tag="RH058_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV3139143.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11164..11973
                     /locus_tag="RH058_003611"
     CDS             11164..11973
                     /locus_tag="RH058_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV3139144.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12009..12863
                     /gene="kdsA"
                     /locus_tag="RH058_003612"
     CDS             12009..12863
                     /gene="kdsA"
                     /locus_tag="RH058_003612"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV3139145.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3235 a         3240 c         3347 g         3113 t
ORIGIN      
        1 cagcggttaa aaaatttggt gaagattttt tgtaataatc ctaattagtt atggggtttt
       61 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
      121 aagcaaaaaa catcgatatt catttttggt caatgggttt tctggcctat cgttgttatt
      181 gtgctactgg ttatcatttt catcttgcca ggttcgcacg taatctcatt attcggggac
      241 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgtg
      301 atcaatattg atcagctgtg aagggggtga tgtgtagtgg aacaaaaact tacgttatat
      361 cactactaat gagatatccg tcatctgaac actgatgtct ttaattattc tggtggttca
      421 ctgtaggcaa taagcacaaa agtgtaggat gttacaagaa tgattaggac tcggtgaaat
      481 gaaaaatcca cgcaattgcg tggattttat atacttttgc gctcttcatg agatttagcg
      541 aaacctcatg agacaacaaa ttaattagac gttgaaaagg aagttcatca catcgccatc
      601 tttaacgatg taatctttac cttctgcacg cattttgcct gcttctttcg cgccttgttc
      661 acctttgtaa gtgatgaaat cttcaaacga gatggtttgt gcacggataa agcctttttc
      721 aaaatcagta tggattttgc ccgctgcctg cggcgcggtt gctccaaccg gaatggtcca
      781 tgcacgcact tctttcaccc cagcggtgaa gtaagtttgc aggttcagca gcttataacc
      841 ggcacggatc acacggttca ggcccggctc ttccagccca agctcctgca taaactcgtc
      901 acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa ccggaaccac
      961 aacagaacct tctttcgccg cgatttcacg cacctggtca agatatgggt tgttttcaaa
     1021 accgtcttcg ttgacgttgg cgatgtacat tgttggtttc agcgtcagga agctcaggta
     1081 acgaatagcc gctttctctt cagcgcttaa atccagcgcg cgcagcatac ctgcgttttc
     1141 cagctggggc aggcattttt ccaggaccgc cagctcagct ttcgcgtctt tatcgccacc
     1201 tttggctttc ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc
     1261 cagttcggtg ttgataactt caatatcgtc cgccgggtta actttgccgg aaacgtgaat
     1321 gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt cacggatgtt
     1381 agtcaggaac tggttaccca gaccttcgcc tttcgatgcg ccttttacca gaccggcgat
     1441 atcgacaaat tccatggtcg tgggaagcgt acgctgcggt tttacgattt cagccagttg
     1501 atccaggcga gggtcaggca ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg
     1561 aaagttggcc gcttcaatac cggctttggt cagcgcgttg aacagggtag atttcccgac
     1621 gttgggcaaa ccgacgatac cgcatttgaa tcccatgatt taaatcacct taatatctta
     1681 ataatcaacc tgttattgct aacagattgc agaaatggaa ataactttgc ctattataca
     1741 cggcactcgg caaaaatgcc gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg
     1801 ttcgttgctt tggtcaagcc atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg
     1861 atggcttcat caattaactt ctgttcacta acaggcggtt tgcctaacac aaaaccgaca
     1921 actttatttt tatcgcccgg atgaccgatt ccgatgcgta aacggtgaaa gttagggtta
     1981 ttacccaatt tactgatgat gtctttcagt ccattgtgac caccatggcc accgcccaac
     2041 ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt gggccaccag aatttcgtcc
     2101 ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag attcataaat
     2161 gtagtcggga ctaacaggcg gacatcttcg cctccaaggg tgactcgcga agtataacca
     2221 aagaatttag cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac
     2281 caggcaccag cattatgtcg cgttgcggcg tattcagcac cggggttcgc caggccgaca
     2341 atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac ataactggcg cgtagtttac
     2401 tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgcc
     2461 tgagtggact attagaaagt caaggtgttc aggcgtttat ttgtaaagtt ttgttgaaat
     2521 aagggttgta attgtgatca cgcccgcaca taacccacag ggtgttgtct atactttaca
     2581 cataaggaag aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa
     2641 cgcaaaaacg cttcgttact cggtaacgtg ctcatggggt tgggtctggt ggtaatggtg
     2701 gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc
     2761 gcacatggtg cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt
     2821 gttggcgggc atgaacaggt gtgcgaccgt tactggtggg ttcgccacta tgacaaacgt
     2881 tgccgccgta gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca
     2941 gtttgcgctg actggccaaa ctcgtactta taaatccgcc atcgccgcgc gacgattcgg
     3001 gaagaacgcg aggcgtcccg ggatcggttg aatgcctgcg cgagccatag tgcgcagagg
     3061 ctggaattcc acgttgcaca cgcgcagttc acatccttcg ggcagacgct tcacaaaacg
     3121 ctggaacgca tcaagaccac cagcatcaag taccggaacg gcatcccact tcagaatcac
     3181 aatccgtttg ccttcaagac gtgactccag gtccgtgaat aagccttcag cagcagcaaa
     3241 aaacagcggg ccaataacgc gcagaaccag gacatcgtct ggaacatcta cgactaccgg
     3301 tgccaggcga gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc
     3361 cacgctgatg gcaataacca tatcaaacag cacggtcagc gacatgcaca gcagcatgac
     3421 gatgatgtca tctttcggcg catgacgcag caagtcgacc actttatgcg cttcactcat
     3481 gttccacgcc accatcaaca gcagggctgc catagcggaa agcggcagcc aggagagcag
     3541 cggtgccagt accagcaggg caagaataac cagaatagag tggatcaccg ccgagatagg
     3601 ggacgttgcc ccggcacgga cgttagcggc agaacgcgcg atggcagctg tagcggtaat
     3661 accaccaaag aacggagcga tgatattccc cagtccttgt ccaaccagtt cgctattcgc
     3721 cttgtgtttc gtcccggtca taccatccag caccacggcg cagagcagag attcgattgc
     3781 gccgagcatt gccattgaga atgccgcagg cagcagtgtg cgaatagaat cccaggttag
     3841 cgtgaattct gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat
     3901 accgttaccc tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac
     3961 atgtccgccg agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag
     4021 gtgaccgggt aaacgaatgc ccagacgcgg ccagaaaaca agaataccta gcgtcacaat
     4081 gccaattgcg gcatcgccca cgttaatggt cggcagcgcc ataaataatg cgccgacttt
     4141 ttgtagataa tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat
     4201 ggtaccgatg gtgatcccga tacccgatgt gaaacctaag gtgacggaaa ccggaatata
     4261 ctcaatcagg cgaccaaagc gtgccagacc catcagaatc aaaaagatcc ccgacagcaa
     4321 ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgac acgggataga gaattaccac
     4381 aaatgccgca gtcggaccgg aaacgctaaa gcgtgatcca cccgtcagag caatgacaat
     4441 ccccgcaaca gctgcagtat ataaaccgta ctggggtgcc acaccactac caatagccaa
     4501 cgccatcgcc agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt
     4561 aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat
     4621 cacatgtgag gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc
     4681 aactaaatgg caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac
     4741 ccgccgcagc gggtctttga gccgggttcg attagtgttc gaacatggca gagatcgatt
     4801 cttcgttgct gatacgacga atcgcttcgg ccagcatacc tgacagggtc agagtacgca
     4861 cgttcggcag tgatttgatt tcatcgctca gcggaatggt atcgcagaca acgacttcat
     4921 caattacaga gttacgcagg ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt
     4981 acgcaaatac acgtttagca ccacgttctt tcagcgcttc agcagcttta cacagcgtac
     5041 cgccagtgtc gatcatatca tcgaccagta cgcagtcacg acctgcaacg tcaccgatga
     5101 tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg
     5161 tatcgttcag cagcttagcg atagcgcggg cacgcacaac gccgccgatg tccggagaaa
     5221 ccacaattgg gttatccaga ttcagctgca gcatgtcttc cagcaggatc gggctaccaa
     5281 atacgttatc aaccggaacg tcgaagaaac cctgaatctg ttcagcgtgc agatccactg
     5341 tcagcacacg gtcaacaccg acgctggaga ggaagtctgc aaccactttc gcagtgattg
     5401 gtacacgagc ggaacggacg cgacggtcct ggcgcgcata gccaaagtag gggataacag
     5461 cggtgatacg acctgcggaa gcacgacgca gggcatcaac cataacgact aattccatca
     5521 ggttgtcgtt agtaggggca caagtggact ggatgatgaa aatatcacca ccgcgtacat
     5581 tttcattaat ttgtacgctg acttcgccat cgctaaagcg acctacagcg gcgtcgccga
     5641 gtgaagtgta caggcggttg gcaatacgtt gtgctagttc cggggtggcg ttaccagcaa
     5701 aaagcttcat atcaggcacg agaagaacct caggcatgcg tccattggtg gaaagaatct
     5761 gccgaaaact gtgcgggcca ggcatgatcc tttccaggcg gtgtattaaa gagcgcgatg
     5821 caacgtctgg aacaaggtga cgttgtcacc gaaactcagc ttgcccggct taaagcatgg
     5881 ctctgtgcaa tggggaaaga ttaacgcctt tcgccacaaa gccattgagc cattccgggg
     5941 cttgctctag cacctggcgg gcttcagact ctgtatcaaa ttcagcaaag acacaggccc
     6001 ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct
     6061 cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct gaattcacat tttagcaacg
     6121 tttctattga cctttttggc gtattgcgcg ggagttcagg atctttaaaa atcaccggag
     6181 tcggaatact tacaccaggg tgcgccacca gataccactt ctctggcgga tccaccggcg
     6241 ttagtatttc accaacgcct tcggcaaacg cggcatgccc ccgaacaaag acaggaacat
     6301 ctgcgcccag cgtcagcccc atttccgcca gctcatccat gcttagcccg cattgccaga
     6361 gatgatttaa tgccaccagg accgtcgcgg cattggatga accaccgccg agaccgccgc
     6421 ccatcggcaa acgcttgtca atgctgatat tcgcaccgct tcccgtcgga agacgcccgc
     6481 tgtctgccgc agttttcatc aacaaccgcg ctgcgcgaac gatcaggtta tcttcatgtt
     6541 ccacgccttc aacgggcgtt aacagacgaa tatccccatc gtcacgaagc tcaatgctga
     6601 tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac
     6661 gctgaccggt aatgtataaa aacagattaa gttttgccgg agagggccac tgtgtccgca
     6721 ttatttcact atccagttat ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc
     6781 catattggct ggcatcgcag gttgcgtttt ggtgtcataa ccaccataaa caaccttcca
     6841 gtttttgcca ttctggctgt aggtaatttc gctcaggcgg tactggtcgt ccagtttgta
     6901 gtcggttgca tcacccggta aacctaaaat ccactggcgc aagctgttga gcggaattgg
     6961 cattccggtc aatttgccaa tcatctcttc ggcgtcatcg gcggtataac gctgaccttt
     7021 attgtcgact aactgcacgt tacccggttg agcattcagc tccagttccg tgctgcccaa
     7081 tgggttagtg agcagcagac ggtagcgatc ctggccggtt tgctgccaga aaaagcgggc
     7141 gtacactttt tgttggtcag aaatataagc gaacgcgccg cgagtctgat actgattaag
     7201 attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa tccgggcttt tgccaggacc
     7261 tttgggcgtg gtaacggaac aggcagtgag cacaagagca gccagcggta gcaggcggat
     7321 aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct tgagatacgt tgcagttata
     7381 acccttaatg ctagcgttac cgtccgctat cgtctatgtt caagttgtct taattgccag
     7441 aatctaacgg ctttcggcaa ttactccaaa agggggcgct ctcttttatt gatcttacgc
     7501 atcctgtatg atgcaagcag actaacccta tcaacgttgg tattatttcc cgcagacatg
     7561 acccttttag cactcggtat caaccataaa acggcacctg tatcgctgcg agaacgtgta
     7621 tcgttttcgc cggataagct cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg
     7681 cagggcggcg tggtgctgtc gacgtgcaac cgcacggaac tttatcttag cgttgaagag
     7741 caggataacc tgcaagaggc gttaatccgc tggctttgcg attatcacaa tcttaatgaa
     7801 gaagatctgc gtaaaagcct ctactggcat caggataacg acgcggttag ccatttaatg
     7861 cgtgttgcca gcggcctgga ttcactggtt cttggggagc cgcagatcct cggtcaggtt
     7921 aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg ccagcgaact ggaacgcatg
     7981 ttccagaaat ctttctctgt agcgaaacgc gttcgcactg aaacagatat cggtgccagt
     8041 gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc agatctttga atcgctctct
     8101 acggtcacag tgttgctggt aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg
     8161 cgcgaacata aagtacagaa gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt
     8221 ctggcagatg aagttggcgc ggaagtgatt gccctgagtg agatcgacga acgtctgcgc
     8281 gaagccgata tcatcatcag ttccaccgcc agcccgttac cgattatcgg gaaaggcatg
     8341 gtggagcgcg cattaaaaag tcgtcgtaac caaccaatgc tgttggtgga tattgccgtt
     8401 ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg cttatcttta tagtgtggac
     8461 gatctgcaaa gcataatttc gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg
     8521 gaaactattg tcgctcagga aaccagcgaa tttatggctt ggctgcgagc acaaagcgcc
     8581 agcgaaacca ttcgcgagta tcgcagccag gcagagcacg ttcgcgatga gttaaccgcc
     8641 aaagcgttag cggcccttga gcagggcggc gacgcgcaag ccattatgca ggatctggca
     8701 tggaaactga ctaaccgctt gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt
     8761 gacggggata acgaacgcct gaatattctg cgcgacagcc tcgggctgga gtagcagtac
     8821 atcattttct ttttttacag ggtgcattta cgcctatgaa gccttctatc gttgccaaac
     8881 tggaagccct gcatgaacgc catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta
     8941 tcgccgacca ggaacgtttt cgcgcattat cacgcgaata tgcgcagtta agtgatgttt
     9001 cgcgctgttt taccgactgg caacaggttc aggaagatat cgaaaccgca cagatgatgc
     9061 tcgatgatcc tgaaatgcgt gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa
     9121 gcgagcaact ggaacagcaa ttacaggttc tgttactgcc aaaagatcct gatgacgaac
     9181 gtaacgcctt cctcgaagtc cgagccggaa ccggcggcga cgaagcggcg ctgttcgcag
     9241 gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg ccgctggcgg gtagaaatca
     9301 tgagcgccag cgagggtgaa catggtggtt ataaagagat catcgccaaa attagcggtg
     9361 atggtgtgta tggtcgtctg aaatttgaat ctggcggtca tcgcgtgcag cgtgttcctg
     9421 ctacggaatc gcagggtcgt attcatactt ctgcttgtac cgttgcggta atgccagaac
     9481 tgcctgacgc agaactgccg gacatcaacc cagcagattt acgcattgat actttccgct
     9541 cgtcaggggc gggtggtcag cacgttaaca ccaccgattc ggcaattcgt attactcact
     9601 tgccgaccgg gattgttgtt gaatgtcagg atgaacgttc acaacataaa aacaaagcga
     9661 aggcgctttc agtgctcggt gctcgcatcc acgctgctga aatggcaaaa cgccaacagg
     9721 ccgaagcatc tacccgtcgt aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta
     9781 cttacaattt cccgcagggg cgcgttaccg atcaccgcat caacctgacg ctctaccgcc
     9841 tggatgaagt gatggaaggt aagctggata tgctgattga accgattatc caggaacatc
     9901 aggccgacca actggcggcg ttgtccgagc aggaataatg gaatatcaac actggttacg
     9961 tgaagcaata agccaacttc aggcgagcga aagcccgcgg cgtgatgctg aaatcctgct
    10021 ggagcatgtt accggcagag ggcgtacttt tattctcgcc tttggtgaaa cgcagctgac
    10081 tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat
    10141 tgctcattta accggggtgc gagaattctg gtcgttgccg ttatttgttt cgccagcgac
    10201 cttaattccg cgcccggata cggagtgtct ggtggagcag gcactggcgc ggttgcctga
    10261 acaaccttgc cgtattcttg atctcgggac gggtaccggg gcgattgcgc ttgcgctggc
    10321 tagcgagcgc ccggactgcg aaattatcgc tgtagatcgt atgcctgatg ctgtctccct
    10381 ggcacaacgt aatgcccagc atctggcgat caaaaatatc cacattctgc aaagcgactg
    10441 gtttagcgcg ctagccgggc agcagtttgc gatgattgtc agcaatccgc cgtatattga
    10501 cgagcaggac ccacatcttc aacaaggcga tgtccgcttt gagccgctca ctgcgctggt
    10561 tgcggcagac agtggaatgg cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct
    10621 ggtatccggc ggctttctgc ttctggaaca tggctggcag cagggcgaag cggtgcgaca
    10681 agcatttatc ctcgcggggt atcatgacgt cgaaacctgc cgtgactatg gtgataacga
    10741 gcgcgtaacg ctcggccgct attatcaatg acaagttttt ctacactgct tagtgttcat
    10801 cttattagta tcgcgctttc tgttgggcta ttaaccttac gtttctggct acgttatcag
    10861 aagcatccac aggcatttgc tcgctggacg cgcattgtgc cgccggttgt cgatacggtg
    10921 ttattgttaa gcggcattgc gttgatggct aaagcgcaca tcctgccatt ttccgggcag
    10981 gcacagtggc tgactgaaaa gctgtttgga gttatcattt atatcgtttt gggttttatt
    11041 gcactcgatt atcgtcgtat gcacagtcag caggcgcgca ttattgcctt cccgctggcg
    11101 ttggtggtgc tgtacatcat cattaaactc gccaccacaa aagtaccgtt actggggtaa
    11161 gtcatgagat cgttagctga tttcgaattt aataaagcgc cattgtgcga aggcatgatc
    11221 ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag atgtttacga cgaactggag
    11281 cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc tgcctttaga agagcagctg
    11341 gaaaaactga tcgcgctgtt ttacggcgac tggggattta aagcctcacg cggcgtttat
    11401 cgtctttccg atgcattatg gctggaccag gtgttaaaga atcgacaggg cagtgcggta
    11461 tcattaggtg cggttttatt atgggtcgcg aatcgtctcg atttgccgct gctgccggtg
    11521 attttcccta cgcagctgat attgcgcatt gaatgtccgg atggcgaaat ttggctgatt
    11581 aatcctttta acggtgaatc gttaagcgaa catatgctgg acgtatggtt aaagggaaat
    11641 atcagcccgt cggcggaact gttttatgaa gaccttgatg aagctgataa cattgaggta
    11701 atccgcaaat tgctggatac actcaaagcc tcgttgatgg aagaaaatca gatggagctg
    11761 gcgttacgca ccagcgaagc tttattacaa ttcaaccctg aagatcccta tgaaattcgc
    11821 gatcgcgggt tgatttatgc gcaactggat tgcgaacacg ttgcgttgaa cgatttaagt
    11881 tatttcgttg aacagtgtcc ggaagacccg atcagcgaaa tgatccgtgc gcaaataaat
    11941 aacatcgcgc ataaacatat tgtgctgcat taattaatcg acattttact caagattaag
    12001 gcgatcctat gaaacaaaaa gtggttagca ttggcgacat caacgtagca aatgacctgc
    12061 cgttcgtact gtttggcggt atgaacgtgc tggaatctcg cgatctggcg atgcgcattt
    12121 gcgagcacta cgtaactgtg acccagaaac tgggtatccc ttacgtgttc aaagcctctt
    12181 ttgacaaagc caaccgctcc tccatccact cttatcgtgg accgggcctg gaagaaggga
    12241 tgaaaatctt ccaggagttg aagcagactt ttggcgtgaa aattatcacc gacgttcacg
    12301 aaccaagtca ggcacagccc gttgctgatg tcgtggatgt gattcagttg ccggcgtttc
    12361 ttgctcgcca gactgacctg gttgaagcca tggcgaaaac cggtgcggta attaacgtca
    12421 agaaaccaca gtttgtcagc ccgggacaga tgggtaatat cgttgataaa ttcaaagaag
    12481 gcggcaacga aaaagtgatt ctttgcgatc gtggtgctaa cttcggctat gacaacctgg
    12541 ttgtcgatat gctgggcttc agcattatga agaaagtgtc tggtaactcg ccggtgattt
    12601 tcgacgtgac ccacgcactg caatgccgcg atccgtttgg cgcagcttcc ggtggtcgtc
    12661 gtgctcaggt ggctgagctg gcacgagccg gtatggcggt aggtctggcg gggctgttta
    12721 ttgaagcgca tccggatccg gaacatgcga aatgtgatgg tccatccgcg ctgccgctgg
    12781 ctaaactgga accgttcctc aagcagatga aagcgattga tgatctggtg aaaggtttcg
    12841 aagaactgga taccagcaag taatcttttt tgcttgaaaa ataaagtctt agcgttctgc
    12901 gttaagactt ttttcatggg tgccagatac aaaaa
//
LOCUS       DAPHDX010000129        12743 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPHDX010000129 DAPHDX010000000
VERSION     DAPHDX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.131"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..470
                     /locus_tag="RH058_003613"
     CDS             <1..470
                     /locus_tag="RH058_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3139146.1"
                     /translation="DYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRA
                     KSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQS
                     KFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQEPTEMIQATA"
     gene            complement(510..1724)
                     /gene="ydcO"
                     /locus_tag="RH058_003614"
     CDS             complement(510..1724)
                     /gene="ydcO"
                     /locus_tag="RH058_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV3139147.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMCIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FAFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1777..2313
                     /gene="sutR"
                     /locus_tag="RH058_003615"
     CDS             1777..2313
                     /gene="sutR"
                     /locus_tag="RH058_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV3139148.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2386..4347
                     /gene="rlhA"
                     /locus_tag="RH058_003616"
     CDS             2386..4347
                     /gene="rlhA"
                     /locus_tag="RH058_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV3139149.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHDWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4439..4669)
                     /locus_tag="RH058_003617"
     CDS             complement(4439..4669)
                     /locus_tag="RH058_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV3139150.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4891..5067
                     /gene="hicA"
                     /locus_tag="RH058_003618"
     CDS             4891..5067
                     /gene="hicA"
                     /locus_tag="RH058_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV3139151.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5113..5529
                     /gene="hicB"
                     /locus_tag="RH058_003619"
     CDS             5113..5529
                     /gene="hicB"
                     /locus_tag="RH058_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV3139152.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5608..7014
                     /locus_tag="RH058_003620"
     CDS             5608..7014
                     /locus_tag="RH058_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV3139153.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7259..8404
                     /locus_tag="RH058_003621"
     CDS             7259..8404
                     /locus_tag="RH058_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3139154.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8422..9435
                     /locus_tag="RH058_003622"
     CDS             8422..9435
                     /locus_tag="RH058_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3139155.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9436..10377
                     /locus_tag="RH058_003623"
     CDS             9436..10377
                     /locus_tag="RH058_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3139156.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPALGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10367..11161
                     /locus_tag="RH058_003624"
     CDS             10367..11161
                     /locus_tag="RH058_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3139157.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11183..12607
                     /gene="patD"
                     /locus_tag="RH058_003625"
     CDS             11183..12607
                     /gene="patD"
                     /locus_tag="RH058_003625"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV3139158.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3083 a         3127 c         3429 g         3104 t
ORIGIN      
        1 gggactacct tcacaaagtc acaacgaccg tcagcaaaaa ccacgcaatg atagtcattg
       61 aggatttgaa ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc cagccggggc
      121 gcaatgtccg ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa
      181 tgcgccgcca gcttgaatac aagcagctct ggagtggcgg tcaggtgctt gctgttccgc
      241 cagcgtatac aagccagcgt tgcgcgtgct gtggtcatac agcgaaagaa aatcgtctgt
      301 cacaaagtaa attcagatgc caggtatgtg gatatacagc gaacgccgat gtaaacggcg
      361 ctcgtaacat tttagcggcg gggcacgccg ttcttgcctg tggagggatg gtgcagtcag
      421 gccgcccgtt gaagcaggaa cccaccgaaa tgattcaggc gacagcctga acgtagcagg
      481 gatccacgtc cttcagggcg tggaggatgt caatatcggt ttctgtcagc gattaaattc
      541 aacaccacgt aacaaacgcc tccggcaatt aatccccaga acgcagaacc aatcccgacc
      601 agcgtcaatc cacttgccgt taccagaaat gccaccaccg ccgcgtcgcg ctcacgctca
      661 ttatgcagcg cctgatacaa acttccgcca atggtactca acagcgccag acctgccagc
      721 atctggatcc aacttacggg cagggcagcc atcatcccgg taatggcact accaaacaga
      781 cctgcgagca aatagaaaat gcctgcaacg gcagctgcca gccaacgttg atctttatcc
      841 ggatgcgctt ccgggctttg gcaaatagcc gcggtgattg ccgcaatacc gacggaataa
      901 acgccgaaag gggaaaaaac cagtgccagc aatccagtaa atacaattaa tggcgaaaca
      961 ggagccgaat atccggcggc tttcattgct gcgatacccg gtgcgttttg cgatgccatc
     1021 gtcaccagaa aaagggggag tgcaacgctc aggctgtgag caaacgcaaa atcaggggta
     1081 atataagtgg ggagaacggg tttaaagaca acatcagttg tgacaacgtc accttgcgcg
     1141 ataacgatca cgatcccaat aatcatcgcg gcaattaccg catagcgcgg cgcaacggcc
     1201 ttggttgcca gccataccag caacatactg ccacacaacg taaattgacc gtccagactg
     1261 gcaaacgcct gtaaaccaaa gcgtaataaa atcccggcaa gcattgccgc cgcaagcgag
     1321 tgcggaataa tgcacatcag acgagcaaag agtcccgtta tgccgcagag gactattaac
     1381 gcgttggtga caataaaaac gccgatggct tcgttaagtg ttagtccctg caatccggtg
     1441 accaacaaag ccgcgccagg cgttgaccat gcggtgagaa caggtacgcg ataccataat
     1501 gtcagagcca gcgtactgac gcccattgcc agccccagcg ccgtcatcca gccagagatt
     1561 tgtgcagtgg tggctccggc gacaatcgct gcttgccaga ttattgccgc tgaactggcg
     1621 tagccaatta ataccgccag aaaccccgcc agtagcgtgg gtggaggaat agaaaacaga
     1681 cgcatagtca cctcgtgcgt tatagcgtac aggcaccgta gcatttgtcc gttataacgc
     1741 acaagtgata aacttccgtt ttgccggagg agtcgcatgg aaaatctcgc tcgcttttta
     1801 tccaccacac ttaaacaact acgccagcag cgcggctgga gtctttcgcg actggcagaa
     1861 gcgacgggcg tttctaaagc aatgttaggg cagattgagc gtaatgagtc cagtccgacg
     1921 gtcgcgacat tatggaaaat tgccaccggg ttgaacgtac cattttcaac atttatttct
     1981 ccgccgcagt ccgctacgcc ttcagtttat gatccacaac agcaggcgat ggtgatcact
     2041 tcgctgtttc cttatgatcc acaactctgc ttcgaacact tctcgattca gatggcatct
     2101 ggtgcaataa gcgaatcgac gccccatgag aagggggtaa tcgaacatgt cgtcgtcatt
     2161 gatggacaac ttgatctgtg cgttgatggg gaatggcaga cccttaattg tggggaaggc
     2221 gttcgatttg ctgcagacgt cacgcatatc tatcgtaatg gtggggagca aaccgtacat
     2281 tttcattccc tcatccatta cccgcgcagt taagcgggaa aactatttcg caacgtcgcg
     2341 cttgtggcta aaatagccgc catttttcag ctactggata agaatgtgac cgtatcttct
     2401 catcgacttg aactgttaag cccggcacgc gatgccgcca ttgcccgcga agctattttg
     2461 cacggtgccg atgctgttta tatcggcggc cctggttttg gtgcccgtca taatgccagt
     2521 aatagcctga aagatattgc cgagctggtg ccgtttgccc atcgttatgg tgcaaaaatt
     2581 ttcgtcacgc ttaacaccat tttgcatgat gatgagctgg aacccgcgca acggctgatt
     2641 actgacctct accagaccgg tgtcgatgcg ctgattgttc aggatatggg gattctggaa
     2701 cttgatattc cgccgattga actgcacgcc agtacgcagt gcgacattcg tacagttgaa
     2761 aaagcgaagt tcctctctga tgttggcttc acgcagattg tgctggcgcg agagctgaat
     2821 cttgatcaga tccgcgcgat tcaccaggct acggacgcga ccattgaatt ctttattcat
     2881 ggggcactgt gcgtggccta ttcgggtcag tgctacattt ctcatgcgca aacagggcgt
     2941 agcgccaacc gtggcgattg ctcgcaggcg tgccgtttgc catacacatt gaaagacgat
     3001 caggggcggg tggtttccta tgaaaaacat ctgctgtcga tgaaagataa cgatcagact
     3061 gccaacctcg gcgcgctgat tgatgctggt gtacgctcct tcaagattga agggcgttac
     3121 aaagatatga gctacgtgaa gaatatcacc gcccattatc gccagatgct tgatgccatt
     3181 attgaagaac gtggcgatct ggcgcgcgct tcatcaggtc gtactgaaca tttctttgtt
     3241 ccatcgacgg aaaagacttt ccaccgtggt agcacagatt attttgtgaa tgcccgtaaa
     3301 ggcgatattg gcgcgttcga ttcgccgaaa tttatcggcc tgccggtggg tgaagtattg
     3361 aaagtggcga aagatcatct cgatgttgcc gtcaccgagc cactggcaaa tggtgatggc
     3421 ctgaacgtga tgattaaacg tgaagtcgtc ggttttcgtg ccaatacggt cgagaaaacc
     3481 ggagaaaatc agtaccgcgt ctggcccaat gaaatgccag cagatttgca caaaattcgt
     3541 ccacatcacc cactaaaccg taatcttgat catgactggc agcaggcact gacaaaaacc
     3601 tccagcgaac gtcgggtggc ggtagacatt gaactgggcg gctggcagga acaactgatt
     3661 ctgaccctca ccagtgaaga gggtgtcagc atcacgcata cgctggacgg gcagttcgac
     3721 gaagccaata acgccgaaaa agcaatgaat aatctgaagg atggtctggc aaaactgggg
     3781 caaaccctct attacgcccg cgatgtgcaa attaatttgc cgggggcgct gtttgtacca
     3841 aacagtctgt taaaccagtt ccgccgtgaa gctgctgaca tgctggatgc tgcgcgtctt
     3901 gccagttacc agcgcggcag ccgtaaaccg gttgctgatc ctgcgccggt ttatccgcaa
     3961 acgcatctga gtttcctcgc gaacgtatac aaccagaaag cgcgtgaatt ttatcatcgc
     4021 tatggtgtgc agctgattga cgcggcgtat gaagcacatg aagagaaggg cgaagtcccg
     4081 gtgatgatca ccaagcattg tctgcgcttt gcctttaatc tgtgcccgaa acaggcgaaa
     4141 ggcaatatca aaagctggaa ggcgacgcca atgcaactgg ttaacggcga tgaagtatta
     4201 acgctaaagt ttgattgccg cccatgcgag atgcacgtca ttggcaaaat caaaaatcac
     4261 atactgaaaa tgccgttacc gggaagcgta gtggcatccg taagtccgga tgagctgctg
     4321 aaaacattgc ctaagcgaaa agggtaaaac gccagttttc tggttactca caactcattg
     4381 aatctgcatg atattgcctg ccgggtaagg cgtctcgccg catccggcaa caaatgactc
     4441 aacgttgtcc cgcttctggt ttgcgcgatt tttgccagta aaaatgctcg cgcaaacctt
     4501 ctgccgactc ttccgccaca gcacgcaatt catcgctgtc cgcttcatga cgcagctgat
     4561 gatccacatt ctttacccac acaaattcat gtcctttgtg ccctgccatg agttgtcctg
     4621 agaacagcgc acacgttaat aagacaaccg ataacgcctt cgtaaacatc ctgccacctt
     4681 tttcttacct tttgccgcta tgatgccgat cgtttcttga ggttattatt cagttttgca
     4741 aattagcgca aagaaattct ggaatcttcc ttcctgattt tgcatggcat tctgccgttg
     4801 cggcgattta gtgctatttt cgagcaaatt acacacggag gtaaacaata atgtttactt
     4861 tgttgatata ctcagcggca gggaggcgat gtgaaacaaa gcgagttcag acgttggctc
     4921 gaatctcagg gcgtcgatgt agcgaatggc agcaaccatt tgaaactcag gtttcatggg
     4981 aggcgcagtg tcatgccgcg tcacccctgc gatgagatta aagaaccatt gcgtaaagca
     5041 atcctgaaac aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgaggg
     5101 acagtcgaaa ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg
     5161 gtttcttttg tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg
     5221 gaagcggcaa aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt
     5281 atccctttac cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc
     5341 gcctctaagg tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta
     5401 gccaggcgaa ttggcaaacc taaacaggag attactcgcc tatttaactt gcatcatgcg
     5461 acaaaaatcg acgccgtcca gctcgcggca aaggcgcttg gcaaagagtt atcgctggtg
     5521 atggtttaat tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa
     5581 tcatctgcac ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta
     5641 cgcgagcaga ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac
     5701 caggtggcgc tttctggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa
     5761 agtctgggat atattatcgc gcgaccgcag tcgggttatt acgttgcgcc acaggcaata
     5821 aaaatgccga aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact
     5881 tatatttttg atatgttgca ggccagtcgc gatccgtcgg tcgttccgtt tgcctcggcc
     5941 tttcccgacc cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc
     6001 aaaaccgcca cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt
     6061 caggctattg ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc
     6121 attactgccg gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc
     6181 gattgggtga tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta
     6241 cggctgaagg cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg
     6301 gaactggcgt tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac
     6361 ccactcggat ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag
     6421 tacaacgtaa cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa
     6481 ccgctgcctg cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg
     6541 aaatttctgg tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa
     6601 attcaacgct tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg
     6661 gtggattacc tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt
     6721 gcggaacgta aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa
     6781 attcatcata gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc
     6841 ggcgaattaa gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt
     6901 tctaccggtg aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa
     6961 cgtgaagaac aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt
     7021 ttaatttaat tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa
     7081 ccccttgtct atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa
     7141 cctgattaaa ttttctcagg ggcgaaggtg tgcctgcaag ccgccgtcta tggttaaaca
     7201 aggagatatt tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat
     7261 gagcaagaca tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca
     7321 cgccgccgaa ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg
     7381 gccgggatac atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga
     7441 aaaagagaca ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag
     7501 tctgatgacc aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt
     7561 gattatgggc aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct
     7621 cgatccgcgc gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc
     7681 ttaccaatgg gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga
     7741 tagctggcaa gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg
     7801 cgttcaggct tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac
     7861 tcagccgcag ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt
     7921 gctgaaagtg ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt
     7981 gcaaatgagc gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc
     8041 caacgccctg aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac
     8101 cggttgggct gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa
     8161 atggatgaac tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc
     8221 gttaccggta gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga
     8281 aaccaacggt tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg
     8341 gggcaagttt gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg
     8401 ctaacttcgc tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg
     8461 tacggtgacg tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc
     8521 tctatgctgg ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc
     8581 gaacagcttt ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg
     8641 tgggagcggg acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt
     8701 cttgacaatg tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca
     8761 atggcgcaag aggcgctgga gaaagtggcg ttggggtttg tacatcaacg taaaccgtca
     8821 caactttctg gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg
     8881 cgcgtattgc tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg
     8941 cagctggaac tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac
     9001 gatcagggcg aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt
     9061 gagcaggtcg attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccgga
     9121 ttcgtcggga catccaacgt ttttgatgga ctgatggcag agaaactttg tggcatgacg
     9181 ggaagcttcg ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc
     9241 aatggcacga tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg
     9301 aacggcggtg aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc
     9361 acgctcacgc ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg
     9421 gttgaggaga ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggt
     9481 aaggtgtccg gtttcttctg gcgtaatccg gcgctggggc tgtttttact gctgcttggc
     9541 ccgctaatgt ggtttggcat tgtctatttc ggctcgctgc tgacactgtt atggcaggga
     9601 ttttatactt ttgacgattt caccatgtcg gtaacgccgg aactgacgct ggcgaatatc
     9661 cgtgcgctgt ttaatccggc gaattacgac atcattctcc gcacgctgac tatggctgtg
     9721 gcagtcacta tcgccagcgc cattctggct tttccaatgg cgtggtatat ggcgcgctat
     9781 accagcggga aaatgaaagc ttttttttat attgcggtaa tgttgccgat gtgggcgagc
     9841 tacattgtta aagcctatgc ctggacgtta ttgctggcaa aagatggcgt ggctcagtgg
     9901 tttttacaac atcttgggct ggaaccactg ctgactgcgt tccttacatt acctgcggtg
     9961 ggcggaaata cgctgtcaac ttccgggctg gggcgctttc tggtgtttct ctatatctgg
    10021 ttgccgttca tgatcctgcc cgttcaggcg gcgcttgagc gtttgccgcc gtcattgttg
    10081 caggcgtcgg ctgatctcgg cgcacgtcca cgacaaacct ttcgctatgt ggtgctgccg
    10141 ctggcaatcc cgggtattgc cgctggctct atctttacct tctcactcac actgggcgat
    10201 tttatcgtcc cgcagctggt tggtcctcca ggatatttta tcggcaatat ggtttattcc
    10261 cagcaggggg cgattggcaa tatgccgatg gcggcggcat tcaccctggt gccgattgtt
    10321 ctcatcgcac tgtacctggc gttcgtgaaa cgtctgggag cgttcgatgc actctgaacg
    10381 cgcaccgttt ttcctcaaac tggcggcctg gggcggcgtg gttttcctac attttcccat
    10441 cctgattatc gccgcttatg cgtttaacac tgaagatgcg gcgtttagtt ttccaccgca
    10501 gggcctgacg ctgcgctggt ttagcgtggc agcacagcgt agtgatattc ttgatgccgt
    10561 gacactgtca cttaaagtgg cggcgctggc gacattaatg gcgctggtgt tagggacgct
    10621 ggcagctgcc gcgctgtggc gacgagactt tttcggcaaa aacgccattt cgctgttact
    10681 gctgctgccc attgcgctgc cgggcattgt cactggtctg gcgctattaa ccgcttttaa
    10741 aaccatcaat ctggagccgg gatttttcac catcgtggtc ggtcatgcga ctttttgcgt
    10801 agtggtggtg tttaacaatg tcatcgcccg ttttcgccgc acctcgtgga gtctggttga
    10861 ggcgtcaatg gatcttgggg ccaatggctg gcaaaccttc cgctacgtag tgttgccgaa
    10921 tctcagttcg gcgctactgg caggaggaat gctggcgttt gccttgtcgt tcgatgaaat
    10981 catcgttacg acttttacgg caggtcatga acgaacgtta ccgttgtggt tgctcaatca
    11041 gcttgggcga ccgcgtgatg taccggtaac taacgtggtg gcactgctgg ttatgttggt
    11101 aacaaccttg ccgatcctgg gggcctggtg gctaacccgc gaaggcgaca atggtcaata
    11161 accactgata caggaatatg ctatgcaaca taagttactg attaacggag aactggttag
    11221 cggcgaaggg gaaaaacagc ctgtctataa tccggcaacg ggggacgttt tactggagat
    11281 tgccgaggca tccgcagagc aggtcgatgc tgctgtgcgc gcggcagatg cagcatttgc
    11341 cgaatggggg caaaccacgc cgaaagtgcg tgcggaatgt ctgctgaaac tggctgatgt
    11401 tatcgaagaa aatggtcagg tttttgccga actggagtcc cgtaattgtg gcaaaccgct
    11461 gcatagtgcg ttcaatgatg aaatcccggc gattgtcgat gtttttcgct ttttcgcggg
    11521 tgcggcgcgc tgtctgaatg gtctggcggc aggtgaatat cttgaaggtc atacttcgat
    11581 gatccgtcgc gatccgttgg gggtcgtggc ttctatcgca ccgtggaatt atccgctgat
    11641 gatggcagcg tggaaactgg ctccggcgct ggcggcaggg aactgcgtag tgcttaaacc
    11701 atcagaaatt accccgctga ccgcgttgaa gttggcagag ctggcgaaag atatcttccc
    11761 agcaggcgtg attaacgtac tgtttggcag aggcaaaacg gtgggtgatc cgctgaccgg
    11821 tcatcccaaa gtgcggatgg tgtcgctgac gggctctatc gccaccggcg agcacatcat
    11881 cagccatacc gcgtcgtcca ttaagcgtac tcatatggaa cttggtggca aagcgccagt
    11941 gattgttttt gatgatgcgg atattgaagc agtggtcgaa ggtgtacgta catttggcta
    12001 ttacaatgct ggacaggatt gtactgcggc ttgtcggatc tacgcgcaaa aaggcattta
    12061 cgatacgctg gtggaaaaac tgggtgctgc ggtggcaacg ttaaaatctg gtgcgccgga
    12121 tgacgagtct acggagcttg gacctttaag ctcgctggcg catctcgaac gcgtcagcaa
    12181 ggcagtagaa gaggcgaaag cgacagggca catcaaagtg atcactggcg gtgaaaagcg
    12241 caagggtaat ggctattact atgcgccgac gctgctggct ggcgcattac aggacgatgc
    12301 catcgtgcaa aaagaggtat ttggtccagt agtgagtgtt acgcccttcg acaacgaaga
    12361 acaggtggtg aactgggcga atgacagcca gtacggactt gcatcttcgg tatggacgaa
    12421 agatgtgggc agggcgcatc gcgtcagcgc acggctgcaa tatggttgta cctgggtcaa
    12481 tacccatttc atgctggtaa gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg
    12541 caaggatatg tcactttatg ggctggagga ttacaccgtc gtccgccacg tcatggttaa
    12601 acattaagtt agatgttagt ggctgttata ggacggccac tatcgtttta ggttctgagt
    12661 ttaatggcgg ttaagtatta gaatgattac attaataact agcgtaaaaa ttaggctaaa
    12721 tgtttgatgg cgatttttca tcc
//
LOCUS       DAPHDX010000130        12687 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPHDX010000130 DAPHDX010000000
VERSION     DAPHDX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.132"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..1571
                     /gene="caiT"
                     /locus_tag="RH058_003626"
     CDS             57..1571
                     /gene="caiT"
                     /locus_tag="RH058_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV3139159.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1602..2744
                     /gene="caiA"
                     /locus_tag="RH058_003627"
     CDS             1602..2744
                     /gene="caiA"
                     /locus_tag="RH058_003627"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV3139160.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2873..4090
                     /gene="caiB"
                     /locus_tag="RH058_003628"
     CDS             2873..4090
                     /gene="caiB"
                     /locus_tag="RH058_003628"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV3139161.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4164..5717
                     /gene="caiC"
                     /locus_tag="RH058_003629"
     CDS             4164..5717
                     /gene="caiC"
                     /locus_tag="RH058_003629"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV3139162.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5826..6611
                     /gene="caiD"
                     /locus_tag="RH058_003630"
     CDS             5826..6611
                     /gene="caiD"
                     /locus_tag="RH058_003630"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV3139163.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6617..7207
                     /gene="caiE"
                     /locus_tag="RH058_003631"
     CDS             6617..7207
                     /gene="caiE"
                     /locus_tag="RH058_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV3139164.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7293..7688)
                     /gene="caiF"
                     /locus_tag="RH058_003632"
     CDS             complement(7293..7688)
                     /gene="caiF"
                     /locus_tag="RH058_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV3139165.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7949..11170)
                     /gene="carB"
                     /locus_tag="RH058_003633"
     CDS             complement(7949..11170)
                     /gene="carB"
                     /locus_tag="RH058_003633"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV3139166.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11188..12336)
                     /gene="carA"
                     /locus_tag="RH058_003634"
     CDS             complement(11188..12336)
                     /gene="carA"
                     /locus_tag="RH058_003634"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV3139167.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2976 a         3298 c         3367 g         3046 t
ORIGIN      
        1 aaataaaaca aacgggaacg caaaataaat attcgttttc acagtggaat taactcatga
       61 agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt ctttccgccg ttaataatcg
      121 tcggcatact ttgttggctt acagtcagag atctggatgc agcgaatgtc gttattaatg
      181 ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat atggtggtga
      241 tgcttttcgg ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg
      301 aaccacctga atttagtacc gccagttgga tctttatgat gttcgcctcc tgtacgtctg
      361 ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc ccgccgtttg
      421 gcttagaacc gaactcgaca ggggcgaaag agttggggct ggcttacagc ttgttccact
      481 ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct tacttcttct
      541 ttgtccgcaa aatggaagtg attcgcccca gctcgacact ggtgccgctg gtaggtgaaa
      601 aacacgccaa agggttgttc ggcactatcg tcgacaactt ctatctcgtc gccttgatct
      661 tcgcgatggg taccagtctg ggccttgcca cgccgctggt gaccgagtgt atgcaatggt
      721 tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc tggattatcc
      781 tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc
      841 gtagttacct gagcttcctg atgctgggtt gggtgttcat tgtcagcggt gccagcttca
      901 tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg cgaatgttgt
      961 tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg ttctactggg
     1021 catggtgggt gatttacgcc atccagatga gtatcttcct cgcccgcatc tcccgtggtc
     1081 gtactgtgcg tgaactgtgc ttcggcatgg tgatggggct gacagcatcc acctggatcc
     1141 tgtggaccgt gctcggtagt aacactctgc tgttgatgga taaaaacatc atcaacattc
     1201 caaatctgat cgaacagtac ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc
     1261 cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc accgttacgc
     1321 tggttaacgc ctgctcttat accctggcga tgtccacttg ccgcgaagta cgcgatggtg
     1381 aagaaccacc gctgctggtg cgtatcggtt ggtcaattct ggttggcatt atcggtattg
     1441 ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc
     1501 cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg aaacagaact
     1561 ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggatttt aatttaaatg
     1621 atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa aactgggagg
     1681 cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc aaagcactgg
     1741 cggatatggg tatcgacagt ctgctgatcc ctgaagagca cggtggtctg gacgcggggt
     1801 ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca acctatgtgc
     1861 tgtaccagtt gccgggcggg ttcaacacct tcctgcgcga aggcacacaa gagcagatcg
     1921 acaagattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg attaccgaac
     1981 cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga aatggtaaga
     2041 tttatcttaa tggtagtaag tgttttatta ccagcagcgc ctacaccccg tacatcgtgg
     2101 tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg tttgttgata
     2161 tgagcaaacc gggcatcaaa gtgaccaaac ttgaaaagct cggtctgcgt atggatagct
     2221 gctgtgaaat cacctttgac gacgtggaac tggacgagaa agacatgttc ggtcgggaag
     2281 gtaacggctt taaccgcgtc aaagaagagt tcgatcatga acgtttcctg gtagccctca
     2341 ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg
     2401 tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc gcccacatgg
     2461 cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg
     2521 gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg
     2581 aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca
     2641 tcagccgctt ctggcgtgat ctgcgtgtag accgcgtctc cgggggatct gacgaaatgc
     2701 agatcctgac gctgggtcgc gcggtgctga agcaataccg ctaagtccgt gcgctgcctg
     2761 atgcgacgct aatgcgtctt atcaggccta cgggagcacg aatgtaggcc ggataaagcg
     2821 tttacgccgc atccggcagt catgcgctac ataaattttt caggagctaa taatggatca
     2881 tctacccatg ccgaaattcg ggccgttggc cggattgcgc gttgtcttct ccggtatcga
     2941 aatcgccgga ccgtttgccg ggcaaatgtt cgcagaatgg ggtgcggaag ttatctggat
     3001 cgagaacgtc gcctgggccg acaccattcg cgttcaaccg aactacccgc agctctcccg
     3061 ccgcaatttg cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg aagcgtttct
     3121 gaaattaatg gaaaccaccg atatcttcat cgaagccagt aaaggtccgg cctttgcccg
     3181 tcgtggcatt accgatgaag tactgtggca gcacaacccg aaactggtta tcgctcacct
     3241 gtccggtttt ggtcagtacg gcaccgagga gtacaccaat cttccggcct ataacaccat
     3301 cgcccaggcc ttcagtggtt acctgattca gaacggtgat gttgaccagc caatgcctgc
     3361 cttcccgtat accgccgatt acttttctgg cctgaccgcc accacggcgg cgctggcagc
     3421 actgcataaa gtgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt
     3481 gatgctgcgt atgggccagt acttcatgat ggattacttc aacggcggcg aaatgtgccc
     3541 gcgcatgagc aaaggtaaag atccctacta cgccggttgc ggtctgtata aatgtgccga
     3601 cggctacatc gtgatggaac tggtgggcat tacccaaatt gaagagtgct ttaaagatat
     3661 tggcctcgca catctactca gtacgccgga aatcccggaa ggcactcagc ttatccaccg
     3721 tatcgaatgc ccttacggcc cactggtaga agaaaaactc gatgcctggc tggcggcaca
     3781 taccatcgca gaagttaaag aacgctttgc cgaactgaat atcgcctgtg ccaaagtact
     3841 gaccgtaccg gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg
     3901 gcaaacgatg gatggtcgca cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa
     3961 ccccggacaa atctggcgcg gaatgccctc acatggcatg gacacggctg ccattttgaa
     4021 aaatatcggc tacagcgaaa acgacattca ggagttggtc agcaaaggtc tggccaaagt
     4081 tgaggactaa acgcgtggcg tcagtcggct tgcctgacta gcgcccgccc aataacaaac
     4141 gaacaaaaat ggatagaggt gcaatggata tcattggcgg acaacatcta cgtcaaatgt
     4201 gggacgatct tgcggacgtt tacggtcata aaacggcgct gatttgtgaa tccagcggcg
     4261 gagtcgttaa ccggtatagt tatcttgagt taaatcagga gattaaccgc acggcaaacc
     4321 tgttttatac gctggggatt cgcaaaggcg acaaggttgc actacatctc gacaactgcc
     4381 cggaatttat cttttgctgg ttcgggctgg caaaaattgg cgcgattatg gtgccgatta
     4441 acgcccgcct gttacgcgag gaaagcgcgt ggatcctgca aaatagccag gcgtgcctgc
     4501 tggtgaccag tgcgcaattc tatcctatgt atcaacagat tcagcaggaa gatgccactc
     4561 aattgcggca catttgcctg acagatgtgg cacttcccgc tgatgatggc gtgagttcgt
     4621 ttactcaact gaaaaatcaa caacctgcca ccttgtgcta tgcaccgccg ctatcgactg
     4681 acgatacggc ggaaattctc ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga
     4741 ttacccatta caacctgcgc ttcgctggat attactccgc ctggcagtgt gcactgcgtg
     4801 acgatgacgt ctacctgacg gtaatgcctg cgtttcatat cgattgccag tgtactgcgg
     4861 cgatggcggc gttttctgcc ggggccacct ttgtgctggt cgagaaatac agcgcccgcg
     4921 ccttctgggg acaggtgcag aagtaccgcg ccaccattac cgaatgtatt ccgatgatga
     4981 tccgtacgtt gatggtgcag ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag
     5041 tgatgtttta tctcaacttg tcggagcagg aaaaagatgc gttttgtgaa cgcttcggcg
     5101 ttcgcttgct gacgtcttat gggatgacgg aaaccattgt gggcattatc ggcgatcgcc
     5161 ctggcgataa acgacgctgg ccgtcgattg gtcgggcggg gttttgctac gaagcggaga
     5221 tccgcgacga tcacaatcgc ccgctcccgg caggtgagat cggtgaaatc tgtattaaag
     5281 gcgtaccagg gaaaaccatc ttcaaagagt attttctcaa cccgaaagcc actgcgaaag
     5341 tgctggaagc cgacggctgg ctacataccg gcgataccgg atactgcgac gaagagggct
     5401 ttttttattt cgtcgatcgc cgctgcaata tgatcaaacg cggtggcgag aatgtctcct
     5461 gcgtggagct ggaaaatatc atcgccaccc atccaaaaat tcaggatatc gtggttgtgg
     5521 gtattaaaga ttcgattcgc gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg
     5581 aaacattgag cgaagaggaa tttttccgct tctgcgaaca aaatatggcg aaatttaaag
     5641 tgccctctta tctggagatc agaaaagatc tgccacgtaa ttgctcgggg aaaataatta
     5701 gaaagaatct gaaataaatg aaacagcagg gaactaccct gccagctaat aatcacgcta
     5761 taaaacaata tgcatttttt gcagggatgt tgtcgtccct gaaaaagcaa aaatggagaa
     5821 aaggaatgag tgaatcatta catctgaccc gcaatggatc aattctggaa attacccttg
     5881 atcgtccaaa agcgaatgct attgatgcaa aaaccagctt tgaaatgggc gaagtatttc
     5941 taaatttccg tgacgatccg caattacgtg tcgccattat taccggtgcc ggagagaagt
     6001 tcttttccgc gggctgggat ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact
     6061 ttggcccggg tggatttgcg ggattaaccg aaattttcaa tctcgacaaa ccggttatcg
     6121 cagctgtgaa cggctatgcc tttggcggcg gtttcgaact ggcgctggcg gcagatttta
     6181 ttgtttgtgc cgataacgcc agcttcgccc tgccggaagc caagctgggc atcgttcctg
     6241 acagcggcgg cgtgctgcgt ctaccgaaga tcctgccgcc tgccatcgtc aatgaaatgg
     6301 tgatgaccgg cagacgaatg ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg
     6361 tggttagcca ggcggaactg atggataacg cccgcgaact ggcgcagcag ctggttaaca
     6421 gcgccccgct ggcgattgcg gcgctgaaag agatcttccg caccaccagc gaaatgccgg
     6481 tagaagaagc gtatcgctat attcgcagcg gcgtgttgaa acactatccg tcggttctgc
     6541 attcggagga tgccattgaa gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga
     6601 aaggacgtta acaaccgtga gctattacgc ctttgagggg ttaattccgg tggttcaccc
     6661 gacggcgttt gtccatccca gtgccgtctt gattggcgat gtgattgtgg gagccggtgt
     6721 ctacatcggc ccactcgcct cactgcgtgg tgactacggg cggttgatcg tgcaagcggg
     6781 agccaatatt caggatggct gcattatgca tggctactgc gacactgaca ccatcgttgg
     6841 ggaaaacggc catatcgggc acggagcgat cctgcatggt tgtgtgattg gtcgcgatgc
     6901 attggtcggg atgaacagcg tgattatgga tggcgcggtc attggcgaag agagcattgt
     6961 tgccgccatg agctttgtca aagcaggctt tcacggcgag aaacgccagt tgttgatggg
     7021 tacgccagcc cgcgctgtac gcagtgttag tgacgacgag ttacactgga aacggttgaa
     7081 taccaaagag tatcaggatc ttgtcgggcg ctgtcatgca tcgttacatg aaacacagcc
     7141 actgaggcaa atggaggaaa atcgcccccg tttgcagggg acgacggatg tgacgcctaa
     7201 acggtgatct tgccggatgc gacctggttg ctggataaga tgctacaaca tcgcatctgg
     7261 caagcaggac gcccggtatc aacgagataa aattaacgac gcatactctt tgacaacatc
     7321 atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat acgcggcgtc
     7381 tttcttacgc cgactaattt atcccggctg ttattgcgca ggcgcgcgta aatttgctca
     7441 tcgatatcga ccacttttac cagtcgctgg cactggcatc cccgtccttc cagcttatta
     7501 gggatcatct taacttcgca gcttatttct gcgacttccg acagaatata agtcagggta
     7561 ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc tatcacccac
     7621 cgattttcag ccatcatcca ttcggcgatt aacaagtaga gtggtttttc aacatatcct
     7681 tcacacatac gaactccagt ttcacaaatt aaaagtctgg ctatgactcg ttttgaagca
     7741 attaaacaag gccatattaa aattgtggat atatcgatag tatgcaactt caccgtcgat
     7801 aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa agatttactt
     7861 aatgggcaaa aaaaacggtc ctcatcagag aaccgtatta cttattcgat caaattagcg
     7921 gatgaaaaat atccgccatg acacgctatt atttgatctg cgcgtgcatt tcctgcaccg
     7981 aaattacttt ttcggtcgcg tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca
     8041 gggtggtgtc ataatgcact ttatattgca gcgcactgcg acggatcacg cgggagtctt
     8101 caatcgcgcg acggcctgaa gtggtgttga tgatgtaggt atattcgcca ttcttgatgc
     8161 ggtcctgaat gtgcggacgg ccttcatgca ccttgttcac cagacgcgga ttaatgcccg
     8221 cttcgcccag cacaatcgcc gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca
     8281 gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa agcaacgcac
     8341 gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcaaac gcttcagcga
     8401 aggtgcggcc cacgcccatc acttccccgg tggagcgcat ttctggccct aacagcgggt
     8461 caacacccgg gaatttattg aacggcagca ccacttcttt caccgagtag tacggcggga
     8521 taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca cgcgccgcca
     8581 cttttgccag cggtacgccg gtggctttgg agacgaacgg aacggtacgc gccgcacgcg
     8641 ggttaacttc aatcaggtag acttcgttgt ttttcaccgc aaactgcacg ttcatcagac
     8701 cgcgcacctg caattcgaag gccagtttct gcacctgctg gcgcatcaca tcctgaattt
     8761 cctgacttaa ggtgtaggct ggcagagaac atgcggagtc accggagtgc acgcccgcct
     8821 gctcgatgtg ctccatgatg ccgccaataa gcaccatttc gccgtcgcag atggcatcca
     8881 cgtcaacttc taccgcgtca tcaaggaagt gatccagcaa cactggcgca tcgttagaca
     8941 cgctgaccgc cgtctggaag tagcgacgca ggtcagcttc gtcatagacg atttccatcg
     9001 cccgaccgcc gagaacgtaa gacggacgta ccaccagcgg gtagccaatc tctttcgcct
     9061 tctctaccgc catttcaata gcggtaacgg tggcgttcgc cggttgtttc agtttcagac
     9121 gctcaaccgc atgctgaaag cgttcacggt cttctgcacg gtcgatagca tccgggctgg
     9181 tgccgataac cggtacgcca gcagcttcca gcgcgcgcgc cagtttcagc ggggtctgac
     9241 cgccgtactg aacgataacg cctttcggct tctcaatacg cacgatttct agcacatctt
     9301 ccagagttac cggctcgaag tagaggcgat cggaagtgtc gtagtcggtg gagacggttt
     9361 ccgggttaca gttaaccata atggtttcgt aaccgtcttc gcgcagcgcc agcgaggcgt
     9421 gtacacagca gtagtcgaat tcgataccct gaccgatacg gttcgggccg ccgccgagaa
     9481 ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca taagtggagt
     9541 acatgtaagc ggtgtcggtg gcgaactctg ccgcacaggt atccacgcgc ttataaaccg
     9601 ggtgcaggtc atactggtcg cgcagcttgc ggatttccgc ttcgcgcacg cccgccagtt
     9661 ttgccagacg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat tcagcgttca
     9721 ggccagtgat gcccacttcc gctactttct cttccagacg caccagttct tcaatctgta
     9781 ccaggaacca acggtcaata ttggtcaggt tgaacacgcc gtccacagac aggcccgcac
     9841 ggaaggcatc ggcgatgtac cagatacgct ctgcgccagc atctttcagt tcgcgacgga
     9901 ttttggttaa cgcttccggg tcatcgaggc tcactttcgg gtcgaatcca gtcgcaccga
     9961 cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca atcgccatca
    10021 cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccagcg aatttttcga
    10081 agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag gaggccggag
    10141 tacgtccgcc agtgatgtcg ttcatcagtt cgtcgagggt gtaacccacc gccagtttcg
    10201 ccgccacttt agcaatcggg aaaccggtcg ctttcgacgc cagcgccgaa gaacgggaca
    10261 cgcgcgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc gcaaactgga
    10321 cattggaacc accggtttca acgccgattt cacgcagcac cgccatcgag gcgttacgca
    10381 tgatttgata ttctttgtcg gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg
    10441 tatggatgcc catcgcatca aagttttcga tagagcagac gatgatgcag ttgtcgtttt
    10501 tatcacgcac cacttccatc tcgtactctt tccagccaat cagcgactca tcaatcagca
    10561 actctttggt cggagagaga tccagaccgc gggcgcaaat ttcttcaaac tcttcgcggt
    10621 tataagcgat accgccgccg ctaccgccca tggtaaagga tgggcgaata atgcacggga
    10681 agcccacgtc agcggcaacc gccagcgctt cttccatcgt gtgtgcgata ccggaacgcg
    10741 cggtttccag accaattttc ttcatcgcta cgtcgaaacg acggcggtct tctgctttat
    10801 caatcgcatc ggcggtcgca ccaatcatag tcacgccgaa ctcttccaac acgccctgac
    10861 gctccagctc cagcgcgcag ttcagcgccg tctgaccgcc catcgttggc agcaccgcgt
    10921 ccgggcgctc tttttcaata atcttgcgta ccacttccca gtgaatcggc tcgatgtagg
    10981 tcgcatcggc catttccggg tcggtcatga tggtcgccgg gttggagttc accagaatga
    11041 cccggtaacc ctcttcgcgc agggctttac atgcttgcgc gccagagtag tcaaactcac
    11101 acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt atatctgtac
    11161 gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact gctcaattaa
    11221 ctcgataaag tggtcgaaca acggcgcggc gtcgtgtgga ccagggctgg cttcagggtg
    11281 cccctggaag ctgaacgccg gtttatcggt gcgatgaatg ccctgtaacg taccgtcgaa
    11341 cagggattta tgcgtgacac gcaggtttgc tggtaatgtt gcttcgtcca ccgcaaaacc
    11401 gtggttctgg gcggtgatca tcaccacgtt tttctcaaca tctttaaccg gatggttgcc
    11461 gccgtggtga ccgaatttca ttttgacagt cttcgcaccg ctcgccagcg ccagcagctg
    11521 atgaccgagg cagatgccga agaccggaat atcggtttcg aggaatttct ggatggcggt
    11581 aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg
    11641 attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc
    11701 tctgtccacc aacatgcgca ggatgttgcg cttggcacca aaatcataag ccacgacgtg
    11761 gaacggcagc tcgtcttctt ttttcgcttc tggcaggccg ccggtcaacg tccagctccc
    11821 ttgtgtccag ctataggctt ctgcggtggt cacttctttt gccagatcca tgccattcag
    11881 acctgggaac gcgcgggctt tttctaacgc caacgccgca tccgggttat cgcctgcgat
    11941 aatgcagcca ttctgtgcgc ctttctcgcg cagtaaacgt gtcagcttac gggtatcgat
    12001 atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt cggtattacg
    12061 gaagttgctg gcaatcagcg gcaggtcgcg aatcaccaga ccttgtgcat gtacctgaga
    12121 agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag taagagtaac
    12181 gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca ttgaagtatt
    12241 gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt
    12301 tccgtcttcc agaaccaata gcgctgactt aatcaaaaca ccctccagag aatattcact
    12361 cactttattt gcatattaat tcacaatgat gacgtaaatc aatgcaaatc tgcttaccga
    12421 cgaatttctg gcaaacggcg gcattctgga gatatgggcg ctaaaagtca acttaatggc
    12481 gggatttttt atattatttt gtggctcatg catgttttac aggcattaag cggcaaaaag
    12541 atcatataac ctgttctgga aaacgcttgc gcagcaagaa gattgcatga aataaaagcg
    12601 agtagggaaa agtggaccaa atggtcaaaa tttacattaa aacaacaaaa accgaagaca
    12661 aaacatgtag tttttggcac ttataga
//
LOCUS       DAPHDX010000131        12477 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPHDX010000131 DAPHDX010000000
VERSION     DAPHDX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.133"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(88..474)
                     /gene="cdiI"
                     /locus_tag="RH058_003635"
     CDS             complement(88..474)
                     /gene="cdiI"
                     /locus_tag="RH058_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-immunity protein system imunity protein
                     CdiI"
                     /protein_id="HDV3139168.1"
                     /translation="MITLRKLIGNINMTKEPEQQSPLELWFERIIDVPLEKLTVEDLC
                     RAIRQNLCIDQLMPRVLEVLTKEPLAGEYYDGELIAALSTIKGEDLKDQKSTFTQIRQ
                     LINQLEPSDINDDLRKDILKINQIIV"
     gene            complement(471..10262)
                     /gene="cdiA"
                     /locus_tag="RH058_003636"
     CDS             complement(471..10262)
                     /gene="cdiA"
                     /locus_tag="RH058_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition effector tRNA
                     nuclease"
                     /protein_id="HDV3139169.1"
                     /translation="MNQPPVHFTYRLLSYLVSAIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGKRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRVTADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSDIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTDASDTLTTQG
                     QITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNSGSL
                     LSGGPLTVNTRDFNQSGRTGAKGKVDITASGKLTSTGSLVSDDVLVLKAQDVTQNGVL
                     SGGKGLTVSAQTLSSGKKSVTHSDAAMTLNVTTVALDGETSAGDTLRVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMVVNASEKLTHSGKSSAPSLSLSAPELTSSG
                     VLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITGDNGLTLNTASLSNPGKIIADTLNVRATTLDGDGLLQGAGALALAGDTLSQGRNG
                     RWLTAGDLSLRGKTLNTAGTTQGQNLTVQADNWANSGSVLATGNLTASASGQLTSTGD
                     IMSQGDTTLKAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTIRQNGVTNSGTLT
                     GIAALTLAARMASPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITSDSLAN
                     SGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLT
                     SAGDITGVDALTLMVNQTLNNHASGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQL
                     TNGGHLQGETLTLAVSGGVNNRSGGVLLSRNALNVSTTTLSNQGTIQGGGGASLNATD
                     RLQNDGKILSGGNLTLTAQALANTGSGLVQAVTLLLDVVNAVNGGRVLATGSTDVKGT
                     SLNNSGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLDAQ
                     DFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAG
                     EAFTNNGTLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLT
                     MNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINTSG
                     NIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGY
                     FTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGR
                     IASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFY
                     GSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSI
                     NNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGA
                     LKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHL
                     FAGLYELPGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTR
                     YVSNAVLSRTGSRYINGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGS
                     ILWWESATINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGGSINAQNG
                     LSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRR
                     QQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGMSA
                     GNDINIAATLISGSKSQSGFWHTDDNSSSSTTSQGSSISAGGNLAMAAGHNLDVTASS
                     VSAGHRALLSAGNDLSLNAVRESKNSRNGRSESHDSHAAVSTVTAGDNLLLVAGRDIA
                     SQAAGVAAENNVSIRAGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDT
                     TVTAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTH
                     TISEDSASREAGSLLSGNRVAVNAGDNLTVEGSDVVADRDVSLAAGNHVDVLAATNTD
                     TSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAG
                     KQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAIN
                     NAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGDPNATGVSLSLTTQK
                     SKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAAN
                     DVLLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVNAAKGSEKGNGTEWT
                     ETTIDSGKTVTINSGRDTVLNGVQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAA
                     GGSFTFGSMTGSGYIAASRDKMKSCFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIA
                     STATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGG
                     HSGHAEGTTQAAVADGTITIRDRDNQKQNLANLSRDPAHANDSISPIFDKEKEQRRLQ
                     TVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQRQEYLAKLRDTQAY
                     RNEMAKYGTGSEIQRGIQAAMAALQGLAGGNMAGALAGASAPELANIIGHHAGIDDNT
                     AAKAIAHAILGGVTAALQGNSAAAGAIGAGTGEVIASAIAKSLYPGVDPSKLTEDQKQ
                     TVSTLATLSAGMAGGIASGDVAGAAAGAGAGKNVVENNALSLVARGCAVAAPCRTKVA
                     EQLLEIGAKAGMAGLAGAAVKDMADRMTSDELEHLITLQMMGNDEITTNYLSSLHDKY
                     GSGAASNPNIGKDLTDAEKVELGGSGSGTGTPPPSENDPKQQNEKPVDKLNQKQESAI
                     KKIDNTIKNALKDHDIIGTLKDMDGKPVPKENGGYWDHMQEMQNTLRGLRNHADTLKN
                     VNNPEAQAAYGRATDAINKIESALKGYGI"
     gene            complement(10275..12047)
                     /locus_tag="RH058_003637"
     CDS             complement(10275..12047)
                     /locus_tag="RH058_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDV3139170.1"
                     /translation="MQRRQNNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITS
                     RAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTEPVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKNTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLSYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGVGTLNPVFTRGMHWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVAALDGGWLHSDRDDPYSSGTLWGGAAGLSTTSDYVSGSFTAG
                     LPLVYPDWLAPDHLTVYWHVAVAF"
BASE COUNT         2540 a         3655 c         3177 g         3105 t
ORIGIN      
        1 catcaggctg gacaggggac tttcaccccc aagctgctga acatgcccgg cacacaaaga
       61 agatctcggc tcagtggccg ggattagtta tacaattatc tgattgattt ttaatatatc
      121 ttttcttaaa tcatcgttaa tatctgacgg ttctagctgg tttataagtt gccttatttg
      181 ggtaaaggta cttttctgat cttttagatc ttctcctttt atcgttgata aagctgcaat
      241 tagttcacca tcgtaatatt cacccgctaa cggctcttta gttagaactt ccaacactct
      301 tggcatcaac tgatcaatac ataaattttg tcggatagcg cggcaaagat cttccactgt
      361 taacttttca agaggcacat ctatgatacg ttcgaaccag agttcaagcg gtgattgttg
      421 ctcaggctct tttgtcatat tgatgtttcc aatcaattta cgtaaggtaa tcatattcca
      481 tatcctttca aggctgattc tattttatta atagcatctg ttgctctgcc atacgcagcc
      541 tgagcttcag gattgttgac gtttttcaac gtatccgcat gatttcttaa tcctctgagc
      601 gtattttgca tttcctgcat atgatcccaa tatcctccat tctctttagg aactggctta
      661 ccatccatat ccttgagagt tccaataata tcatgatctt tcagagcatt ttttatagtg
      721 ttatcgatct tcttaatcgc actttcttgc ttctgattaa gcttatctac aggtttttca
      781 ttttgctgct taggatcatt ttccgatggt ggtggtgtac cggttcctga gccggaaccg
      841 cccagttcta ctttttccgc atcggtcaga tctttaccga tattcggatt cgaggcagcc
      901 ccggaaccgt acttatcatg caacgaactg agataattag tagtgatctc atcattaccc
      961 atcatttgca gggtaatcag atgctccagt tcatcggagg tcatcctgtc ggccatatcc
     1021 ttgactgccg ccccggcaag cccggccatg cccgctttcg ccccgatttc tagcaactgc
     1081 tctgcaactt tagtcctgca aggtgctgcg accgcacagc ctctggcaac cagactcagc
     1141 gcattattct caacaacgtt cttcccggca ccagctccag cagccgcgcc agccacatcg
     1201 ccactggcaa tgccgccggc catacccgct gacagcgttg ccagcgtgct tacagtttgc
     1261 ttctgatctt ctgtcagttt cgacggatct acgcccgggt agaggctttt cgcaatggct
     1321 gacgcgatca cttcgccagt acccgcgcca attgcgcctg ctgccgcact gttgccctga
     1381 agggctgctg tcacaccacc gagaatggca tgggcaatgg cttttgccgc tgtattgtca
     1441 tcaatacccg cgtgatgacc gatgatgttc gccagctccg gcgctgaagc acctgccagc
     1501 gcgcctgcca tattgccgcc cgccaggccc tgaagcgctg ccatcgcggc ctggataccc
     1561 cgctgaattt cactaccagt gccatacttc gccatttcat tcctgtaagc ctgcgtgtca
     1621 cgcagtttcg caagatattc ctgacgttgt ttctctgttg cattagcagg aagtgtttcg
     1681 ccggttgctt ccttcgcggc cttcaacgca ttcagttctc cctgtgtacg cgcaatatcc
     1741 gccacctgac tgcctatgtc actgataagc cccactgtct gcagacgcct ctgctctttc
     1801 tccttgtcaa atatcgggct gatactgtca ttagcgtgcg cagggtcacg gctcaggttc
     1861 gccagattct gcttctgatt gtccctgtcc cggatggtga ttgtgccatc tgccactgcg
     1921 gcctgagtcg ttccttccgc atgtccgctg tgacctccgg cggatatcat gccacccggc
     1981 atgttaccct gaaatttatc cccgaagctg ccaccaccgc tcagactgat tccactgtga
     2041 ctgactttat aatccgcttc gttgtgaagg tcactgaacc ccagcgttcc ggtatccagg
     2101 tggtttttat ccggtgtggc agtggaggca atcaccgcac catccagttg ggtatgtctg
     2161 cccactgtaa tgtcgaagcc accatcaccg gcaaacattc cggtttgttc agcaacggag
     2221 tcaaagcagc tcttcatctt atcccgggag gcagcgatgt aacctgagcc ggtcatggag
     2281 ccaaaggtaa aactgccgcc ggcagccacg ctggtctgtt tactgtcgta cttactggtg
     2341 tcctgctggc tgcttatcag caggtcgtgg cccacatcgg cgataatcct gttgccgttg
     2401 acctgaacac cgttcagtac cgtatcccga ccactgttga tggtgacggt tttaccgcta
     2461 tctattgtgg tttcagtcca ctcagtaccg ttacctttct cgctgccttt tgccgcatta
     2521 acgctggcaa agacgctgat accggcaccg ttaccaccgg caccgatact gacacccacg
     2581 ccaccgccac tgctgctgtt cctgcccgtt gttttttgtg tgtttgcagc gccactcaac
     2641 agaacatcat tcgcggcatc caggcttgtg ttaccaccgg ccttaagctg gcttcctgca
     2701 atcacaatat ctccgcggtt atcgcccctg tttttgccgg ttgcgacaac agacagatta
     2761 ttcccggcat tcagcgtact gccggatact gtgtcacttt cagaatgttg ttgtgatttc
     2821 gatttctggg tggtaagcga caggctgact cccgtcgcat tcgggtcacc ggttgcggtg
     2881 gccattgccg cagcctgtcc ggcctgcaca ccagacagcg ctgtctttgt ggcctgcagg
     2941 gctttcagac ggctgtcact gctctccttc gtctcctgtg cactggtgac cgcattattg
     3001 atggcactgc ccaccgtgcc ggaaagggca accgtcagcc cgcttttctt ctgctcaaat
     3061 ttttcgtcca cagtacgacg gtcatgcccc gggtcaacca ctacactgtc accggtaatg
     3121 ctgatatccc ggttcgcaat cacatccgaa ccgctgatat gagcctgttt gcccgcggta
     3181 atactgacat taccggcagt ggagccgatg gtgctggcac tctgactctg cgttgtcccg
     3241 gcctcgcgcc ggtcgtgcgt tgtcttactg ctgccaatgg tgaaaccaat accgccggta
     3301 cccatcagac cggatttctt cgtttcctta aagcgccagg acgtatctgt attggtggca
     3361 gcaagaacat caacatggtt acccgccgcc agtgacacat cccggtcagc caccacatcc
     3421 gaaccctcta ccgtcaggtt atcaccggcg ttaacggcca cgcggttccc cgacagcagg
     3481 gagcctgctt cacgggaggc actgtcctca ctgatggtgt gggtggtttt cttactgaga
     3541 aaacctccgc tttttttctt cgtttccaga tagtgatagt cactttctgt cgccgtggtc
     3601 agggcaacat cacgaccggc attcacgctg atattgccgg ttgcggtaac ggatgacgca
     3661 acagcggtga tatcccgtcc tgcggtgacg gtggtgtcac caccgctggc aatttccgtt
     3721 ccctgctgac ggactgtctc gttaatctct ttctttttct tcgacgtata gctgtcacct
     3781 gcgccggcag actctgccac caggttcaca tcacgcccgg cccggatgga cacgttattt
     3841 tccgcagcca caccggcagc ctgactggca atatcacgac cggcaacaag gaggaggtta
     3901 tcgcccgccg tcaccgtgga cactgctgcg tggctatcat gactttctga cctgccgttg
     3961 cgactgtttt tgctttccct gactgcattc agactcaggt cgttacctgc agaaagcagg
     4021 gctctgtgcc cggcagaaac agaggatgcc gtgacatcca gattatggcc tgcagccatc
     4081 gccaggttac cgccggcgct gatgctgctg ccctgtgagg tggtggatga tgaactgttg
     4141 tcatcagtgt gccagaaacc ggactgactt ttgctcccgc ttatcagggt tgcggcaatg
     4201 ttgatatcat tacccgcaga cattccaagg gttccaccgg acgagaccgt tgccccggta
     4261 atatcaatgt ttttccctgc atccagtgaa agtgaatcag tgcctttaat ggtcgcaacc
     4321 ggaccggtgt ccgtaccgct gagatgcaca ccaccatatc ggctgtcact gcccgcattc
     4381 cattgctgac gccgggtgat attgctgatg ttgccactca cgctttccag ttgtacggtt
     4441 ttaccgctga tgactgagct gatattgctg atatccccga tggcgctcag gtccaggcta
     4501 ccgcccgcgc ttatcagccc cgcattcagg ttgtcgatat agccggtact gtcgagcgag
     4561 aggccgtttt gtgcgttgat gctgccgccg ctgttggtga tattgccacc cgcaagctgc
     4621 acgttgttcc cgctgataac gctgccgtta tgcagggtga tatcttccgg cgacagatac
     4681 agtttcggga ccatgactgt ctgtccgttg atggtggctg actcccacca cagtatgctg
     4741 ccgtcaagct gagcaatctg ttcagctgtc agcgccacac caaactctaa tcccagtcct
     4801 ttctgttgtc tggccgcgtt atccatcaga taccgcatct gttccgtgtc tgaacccagt
     4861 ccgttgatat aacgtgaacc cgtccggctc agcaccgcgt tactgacata ccgggtatca
     4921 aagaccgcat cccccaggaa acgataatct ttttccggtt tcagcccgag gcggtcaaga
     4981 aaatacgatg agcccagaaa ctgtttttca tcggtatacg acggagccgt ttcacgtggc
     5041 gcctgacccg gcttcgctcc aggaagctca tacagtccgg caaacaaatg gctgtccacc
     5101 tgtccgagac catccagttt cgggtttacc gtaatcagat acggactgtc cgggtccgtg
     5161 gacggaacca ggtatccatt gttgccggaa ggcagtggcc agtcatcact gataccggtc
     5221 tgaccggtca gtggcgaacc tccggcaata tttttcaggg cacctgccag ttcatcgtgc
     5281 cattgcggag agccaaccac caccggctca tactgctgca gcgctgtctg tgtcagactg
     5341 tctccgccgg tctgctgact taacgtattc agtaccggtg cagagaccac cggactgaca
     5401 ctacctgcat gtgcagtggt tgttccgtta ttgatactgc tggtaaaacg ggtcttaaca
     5461 tccccgcccg cctgaataac ggaataatac gtcttaccgg gcgtgtaatc tttttcccgg
     5521 ccatccagtg aaaatctgat ggtattgttt tcaaattccg gtgacagcag gggcagttta
     5581 tccagagagc ctgtggcata gctaccgtaa aacgttttcg ggtcgtagcg gtataccaga
     5641 tattcattct ctgtccccgt ctgccagctc tgattgctta actctctgcc cgaaagtgcg
     5701 atatccccat tcgccaggat aaatgacgcc cggttttcca gtcgttcagc ctcagcagaa
     5761 agattacgcc ctgacgcaat gcggcctgcc ggattatcag caccggttac tgttgtgatg
     5821 ttctggctgc tgagaaagcg ctgtgtggca ctgtcagcaa acggcgcgta ataataaagc
     5881 gtatccattg tgatattgca tgccccgtgc ccgttgcagg gcgtaccgtg ctgattttca
     5941 acttcacggg tgaaatagcc atagctgccg tcaggaagaa gggaaagggg aatatcaacc
     6001 agagcatttc ccattccctg aatggatgag gggttagtcc gggttgttgt tgtggcagaa
     6061 aatccctccc gctggttcag aaggtgcccg gttcttacaa caatatcgcc ctgatgcgtc
     6121 tcaatattcc cggaggtatt gataatctct gtgtttgcac cgccggaagc atccttctgt
     6181 acccacagac tgttgccggc caggatatca ccatgctggt tgtgcagacg gtctgtaaac
     6241 agcttcaggt tattccccgc ataaatcagc gcactgttca gcagggtacc ggccacattc
     6301 attgtcagac tgcctgccgt gccggtaaaa ccactgatgg tgatatcact ccggctgttc
     6361 agactcacat cgccaccggc ctgaagtgaa cccggtgcgt taaggaaaag acgctgtgcg
     6421 ctgaaaacac tgttgccttt accggcagtc agcgttccat tgttggtgaa tgcctctccg
     6481 gcaccgagca ccatggcatc accctgcatg acaccgccgt tggtaatggc attttgcgac
     6541 gtgacggaaa gggttttccc tgcggccagg gtaccatgat tcgtgagggc agcaatcagt
     6601 ttcagtgtga catcaccggt ggccaccacc tgaccctgac cactgaagtc ctgagcgtca
     6661 agcagcaggt tgcctgcact gtacagccgc cctgtaccat tttgcagcag tgaactgccc
     6721 ttgacgccaa gcccggaggt tcccagcagg gtaccgctgt tgctgaatgt gtggtaattc
     6781 accagcaggt ccgcaccctg aagcgtaccg ctgttattca gcgaggttcc tttaacgtcg
     6841 gtactgccgg tggcaagtac gcgtccgccg ttgacagcat tcaccacatc cagcagcagg
     6901 gtgacagcct gtaccaggcc gctgccggtg ttcgccagcg cctgcgcagt cagcgtcagg
     6961 ttaccaccgg agaggatttt gccgtcgttc tgcagacggt cagtggcgtt cagggaagct
     7021 ccgccaccac cctgtattgt gccctggtta ctcagggtcg ttgtgctgac attcagtgca
     7081 ttccggctca gcagaacacc accggaacgg ttgttcacgc caccggagac tgccagcgtc
     7141 agcgtttcgc cctgcagatg cccgccgttt gtgagttgtc ctgccgtgat ggtggtggca
     7201 tttccctgta attgcccgtc gtttgtgaca ctgtctgcct tcagcgtcag cacacctgca
     7261 ctgagcagtt ttccgctcgc gtgattgttc agcgtctgat tcaccatgag cgtgagagca
     7321 tccacaccgg tgatgtcacc cgcactggtc agtgagttcg ccttcagggt cagatttttt
     7381 gcaatccatt gtccgctgtt gcttaaattc agtgcactga gcgccatttc accgttcgag
     7441 gtgactttgc tgcctgctgt gctgacaagc tcacccgtca gacgtgcagt caggctgtca
     7501 gccgcctgga tcgccccgct gtttgccaga ctgtctgagg tgatcagcac ccgtttgccc
     7561 tgccagtgtc cggaactggt aatactgcct gcggtaattg tcagatcgcc gctggtcagc
     7621 agtgaacctc cgttattcat cagcgcaggt tgaggggatg ccatacgggc ggcaagcgtc
     7681 agcgcggcga tcccggtgag cgtgccactg ttggtgacac cgttctggcg aatcgtgaca
     7741 tggttaccct ggacagtgcc gctgttatcc agtgaattac catcaaggga gagcgtgccg
     7801 gccgaaagca gactgccccg gttgtccgtg gtggctgctt tcagcgtggt gtcaccctgg
     7861 ctcatgatat cgccggtact ggtcaactga ccgcttgccg aagcggtaag gttaccggtt
     7921 gccagcacgg aaccactgtt cgcccagttg tccgcctgca cggtgagatt ctgtccctgc
     7981 gtggtccctg cagtattcag tgttttaccc cggagggaga ggtcgcccgc cgtcagccag
     8041 cgtccgttac ggccctgtga gagggtgtcg cctgcaagcg ccagtgcacc ggcgccctgc
     8101 aacaggccgt cgccatccag cgtggtcgcc ctgacgttca gcgtgtcagc gatgattttt
     8161 cccggattgc tgagggaggc agtgtttaac gttaaaccat tatcaccggt gataagcccg
     8221 ctgttgcgga tgtccggtat atccagcgtc aggtctgcag cactgtacag cgtgccgttc
     8281 tgctgattat caagcctctg tgtgttaacg gtgagtgagg cctccccctg caacagaccg
     8341 ctgttggtca gggtctgtga ctgtgtattc agggcggaac caacaagtac gccactgctg
     8401 gtcagttccg gcgcactgag gctgagcgac ggggcactgc ttttcccgct gtgggtgagc
     8461 ttttcactgg cgttcaccac catggtctgt tgtgctgcct gcgtacctgc aagacgtgca
     8521 tctctggcgt tgatgctgag atttttgccg ctctgaagtt gtgcgcccgc tgcggtactc
     8581 agtttgtctg cctgaacccg gagggtgtca ccggcactgg tttccccgtc cagcgccact
     8641 gttgtcacat tcagcgtcat cgcagcatcg ctgtgggtga ccgatttttt accggagctc
     8701 agcgtctgcg cactgaccgt cagccctttg ccgccggaca gcacaccgtt ctgtgtcaca
     8761 tcctgcgcct tcagcaccag tacatcatcg ctcaccagcg agcctgtact ggtcagtttc
     8821 ccgctggccg tgatatccac tttgcccttc gcgccagtgc gtccactctg gttaaagtcg
     8881 cgggtattca cggtcagggg accgccactg agcagggagc cactgttgct gagcgttgta
     8941 ctgccgagcg tcagggaagc cccctgaaca gcaccactgt tattcagcgt gccggcatcg
     9001 agtcccgcat gacctttcgc cagcaatatt ccgtcctgtg tcagcgtggt ggcgctggcc
     9061 gtgagattct gcccggcggt tatctgtccc tgggttgtca gcgtgtcact ggcgtcagtc
     9121 acaatatcgc gggccgcgtt aatctggctg gcggtatcct gtgtgatgtt tttcgcggca
     9181 agcgttacat cccggccggc agtcagtttt tcattctgtt gagtgattct gccgccggcg
     9241 gtcaggctga ggtccttgtc gctgttaagc gtgccattgc tgagaacgat atcgctccgg
     9301 ctgctgacgc tcaggttacc tcccgcttta tggtcgccgg ttaaggtgac gccctgacct
     9361 tttgcagtga cggcccccgt ggcgagactg ttgttgacag tcagtctgcc gctggcatcc
     9421 agggtgatat cgccatcgcg ggcataaagg ttaccaagat taaccccgac accactttca
     9481 gtggaggtca gatgaatacg cctggcgtac attccaccga gcgcgccggt atcaacggca
     9541 actttcggca catcaccttc gcctttcagg gcactgacac gaccatctgc agttacacgg
     9601 ttagcgcctg cagtgacagt taaatccttc gcatgaagcg cggcattcac ttccgttgca
     9661 cgggcaataa tggatacggc atcgctccgg gtgccgtcca ggcccgcgcc attgatggtg
     9721 atactgcctt cagtcacctc cagcgcctgc aggctgccgt cggcattcat cacaggtctg
     9781 cccgtggtga gcgtcgcgtg cggcgtgttg ataaaaccac agccgtcaca ggtgatacca
     9841 tacgggttgg caaccatcac attcgccgct ttgccggcca cttccgtata gccctgcagc
     9901 agtgaacgct taccgccggt cacttcgttg atgataccct tcgcttcccc gcccgctttc
     9961 aggttcgggt tattctgtat cagtccacca agctgcgtcg gattaagctt accggtggca
    10021 ttattgagaa tcagcccttc cttcccgacg ttgtaatccg taaaccggtt atgcgaaatc
    10081 ccggccccgt tcggcgtggc aatgttcacg accggcacac catttgccgc tttatccatt
    10141 ccggccccgt tttgtggggt gatgacggcc cccacagccg gtaacaacgg ctgcccggcg
    10201 ataatcgcac tgaccaggta actcagcagg cggtaagtga agtgaacggg aggctgattc
    10261 atggtaataa tcccttaaaa cgcgacggca acgtgccagt aaaccgtgag atggtcaggg
    10321 gcaagccagt ccgggtaaac cagaggcagc ccggcagtga acgaaccgga aacatagtca
    10381 ctggtggtgc tgagcccggc agcaccaccc cacagcgtgc cggacgagta cgggtcatct
    10441 ctgtcagagt gcagccagcc gccgtccagt gcagccactg cacggacagt tcccacatat
    10501 ggcagggaga acagagacca ggacagctca tttcgcagat aaccgccgtt attaccggag
    10561 atatactgct ccttaaagcc acgcactgaa ctctcacccc cgaggctcag ttgttccaca
    10621 ccatgaagac ggtccggtga ccactgggca taagcgctgg tcagccacca caccctgtcc
    10681 gtgacggggc gctgaaaact ggcactcacc gaccatttcc ggaactgatt tacgggcagg
    10741 tctccccttt tcccgtggtc gctttctgcg ccgaaccagt gcatcccccg tgtgaatacc
    10801 ggattcagtg ttccgacacc ccccagaaac ttgtgtgtgt gattcagccc gacagaaaat
    10861 gaagtgagtt tacggctgct gccctgaagc agaacatcat ccagataatt gtgaataatg
    10921 cggtgctgca gacctccggt cagtgctgtc ttcatgtccc cgttacggaa caggacatgc
    10981 gacagtccca gccggtgagt ctgcaggtct cccgtggaac gccaccgcca gccccggtta
    11041 tcaatggtgc tgaggtagtc actccatgaa tacgtataat ccaccagggt atagccatac
    11101 gacagactga caccggcggc aaaattcctc gcatcatgtg acaccgaaaa gtcactgctc
    11161 cgtcccccgc tgacaaacca gttgtcagcc agccccagcg gattattaaa ggaaaggaca
    11221 ccatttaact gccccgtacc ggtattcttc tgcccgctgt tgtcgatacc cacgctccct
    11281 gtgacgggcc attccggcaa tgccgtcagt gtcaccaccg accatccctc acggtcaccg
    11341 ggcgatattt caatctgtac cggctccgta cgcagacgat taatctgctc catcccctgc
    11401 tcaatatccc gcaggttcag aaccttcccc tccattcccg ggaaaaccat cttcagggtg
    11461 cgggcaggaa ggtcagcgcc ttccgcccgg atttgctgca gcctgccttc catgaccgtt
    11521 atgtgcagta cgccccctga aaggtcctgc tctgtcagaa aggcccggct ggtgatatat
    11581 ccccggcgta tatagccgtc cgtcacggca tccgtgaccg cagtcaggcc ggtgatattc
    11641 agacactgat tcacccacgg tgccaccagt ctgtcggttt ctgcagacgt cagtcgggtg
    11701 gccccactga caacaatgcg tgacaccgta aagcaggggc cttcagtacc ggcagacgtt
    11761 tccggagacg gcgtgatagt cagcggcgca ctgcgctcca gcgcatcacg ctgacgctgg
    11821 ttttcatcca gcaactgctg ctgttgctgc tgaattgcac tgcggtcacc cggagacagc
    11881 atggctgccg gagacgaaaa tgcggggata aaaaaaccag aaaaaacgga gagggagaga
    11941 tagcatatcc acggagagcc gcgccggaac atatcacaac aggggacaga caacgcacgg
    12001 gataaaaggg acgttttcgt cagaatgttg ttctgccggc gctgcatgtt ctccccgtat
    12061 tctggcctgt gtgatgccag tgtaaattca tgtcaaaaag tgtcagcgag aaaatttaca
    12121 cctgaaaaag tctgtcatga aacagtttta tgacaagaca tcaaaaaaat gtcaaaaacg
    12181 tgatctttct tcagcttttc agaatattga gtaccaggaa gtcaatatca attctgggac
    12241 acatatgcat ttaccattaa aaccgggcac aacaagctgt tcagtctcaa tccatacccc
    12301 gtgttttatc aatatcagaa taagaatatc tgcggataaa taaaaaagga atgtccaggg
    12361 agcttaccgc agagggctct ccacctcaca aaccatttac aaaaatgata ccggcatatc
    12421 tttggtccct gtcacaacca gacattgaca atccaatata accccaaata tatttcg
//
LOCUS       DAPHDX010000132        11863 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPHDX010000132 DAPHDX010000000
VERSION     DAPHDX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.134"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            275..1663
                     /gene="narU"
                     /locus_tag="RH058_003638"
     CDS             275..1663
                     /gene="narU"
                     /locus_tag="RH058_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV3139171.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNTNTPFGVFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1745..5485
                     /gene="narZ"
                     /locus_tag="RH058_003639"
     CDS             1745..5485
                     /gene="narZ"
                     /locus_tag="RH058_003639"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV3139172.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGAI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIESIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5482..7026
                     /gene="narH"
                     /locus_tag="RH058_003640"
     CDS             5482..7026
                     /gene="narH"
                     /locus_tag="RH058_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV3139173.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7026..7721
                     /gene="narW"
                     /locus_tag="RH058_003641"
     CDS             7026..7721
                     /gene="narW"
                     /locus_tag="RH058_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV3139174.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNHYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7718..8398
                     /gene="narI"
                     /locus_tag="RH058_003642"
     CDS             7718..8398
                     /gene="narI"
                     /locus_tag="RH058_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV3139175.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8478..9371
                     /locus_tag="RH058_003643"
     CDS             8478..9371
                     /locus_tag="RH058_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV3139176.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHNDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9467..10312)
                     /gene="nhoA"
                     /locus_tag="RH058_003644"
     CDS             complement(9467..10312)
                     /gene="nhoA"
                     /locus_tag="RH058_003644"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV3139177.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10485..11054
                     /locus_tag="RH058_003645"
     CDS             10485..11054
                     /locus_tag="RH058_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV3139178.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11058..11285)
                     /gene="pptA"
                     /locus_tag="RH058_003646"
     CDS             complement(11058..11285)
                     /gene="pptA"
                     /locus_tag="RH058_003646"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV3139179.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11339..11863)
                     /locus_tag="RH058_003647"
                     /pseudo
     CDS             complement(11339..11863)
                     /locus_tag="RH058_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2802 a         3076 c         3090 g         2895 t
ORIGIN      
        1 agttaatatg taacccttta gattcacgat ctgaagggtt gaaacatgac atacgttcaa
       61 agattaaccc ccgtttgaag gttagcttgt tagttacatt tagtaacaca tatctggcta
      121 cttaatcccc attctgcccc cgcggttacg ctttcttatc tattctttca atcagcactt
      181 cacccaattc atccttaatt ctaataactg gctattgcgc cttacctcct gttttgtctt
      241 ttttcattac caatgtgtgc atgtgaggaa caatatggca ctgcaaaatg agaaaaatag
      301 tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctgggaaa ataaaggaaa
      361 acatattgct cgaagaaacc tctggatatc agtcagttgt ctacttcttg ccttctgtgt
      421 ctggatgcta tttagcgcag ttaccgttaa tctcaataaa atcggtttta attttactac
      481 cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat tgcgtgttcc
      541 ctactccttt atggtgccta tattcggtgg acgccgatgg acggttttta gtactgcaat
      601 cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa aatacgaata ctccttttgg
      661 ggtatttatc gttatcgctt tgctatgcgg ttttgcaggt gcaaactttg cttcgagcat
      721 gggcaatatc agtttcttct ttccaaaagc caaacaaggg agcgctcttg ggattaatgg
      781 cggattagga aacttaggtg taagtgtaat gcagctggtt gcaccgctgg tcatttttgt
      841 acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag gccgacggtt cggtgatgtc
      901 gctggcgaat gccgcatgga tttgggtacc gctactggcg attgccacga tcgccgcctg
      961 gtcagggatg aatgatatcg ccagttcacg cgcctcaatt gccgaccagc tccctgtctt
     1021 acaacgcctg catctctggc tgctgagcct gctttacctt gccaccttcg gttcgtttat
     1081 cggtttttct gcgggttttg ccatgctggc aaaaacccag ttcccggatg tgaatattct
     1141 gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cggtcggttg gtggtgctat
     1201 ttccgataag ttcggcggcg tgcgggtgac gttgatcaac tttattttta tggcgatttt
     1261 cagtgccctg ctgttcctta ccttaccggg cacaggctcc ggtaatttca tcgcctttta
     1321 cgccgtattt atggggctgt ttctgactgc gggtctggga agtggttcta ctttccagat
     1381 gatcgccgtc atctttcgcc agataaccat ttatcgggta aagatgaaag gcggtagtga
     1441 tgagcaagct cagaaagaag ccgtcaccga aacggcggcg gctctgggct ttatctcagc
     1501 cattggcgca gtgggcggct tttttattcc gcaggcgttt ggcatgtcgc tcaatatgac
     1561 cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctgct
     1621 gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgatg
     1681 tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga gcaggagtca
     1741 tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg aaacctttgc
     1801 cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct atcgccagcg
     1861 ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag gctcctgtag
     1921 ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga ccgactaccc
     1981 gcgcactcgc cctgacctgc ccaatcatga acctcgcggc tgcccgcgtg gcgcaagtta
     2041 ctcctggtat ctttacagcg ctaaccgcct gaaatacccg ctcattcgta aacgactgat
     2101 tgaactgtgg cgcgaagccc tcaagcaaca cagcgatccg gtattggcgt gggcatcgat
     2161 tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg cgtgggcgcg gcgggtttat
     2221 ccgctccaac tggcaggaac taaaccagct gattgccgcc gctaacgtct ggaccatcaa
     2281 aacctacggc ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt cgatggtttc
     2341 ttacgccgcc ggaacgcgtt atctgtcact gcttggcggc acctgtttaa gcttctacga
     2401 ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgagcaaa ccgacgtacc
     2461 ggaatctgcc gactggtata actccagcta catcatcgcc tgggggtcta acgtaccgca
     2521 gacacgtacg ccggacgccc acttctttac cgaagtacgc tacaaaggca ctaaaaccat
     2581 cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc tggcaccgaa
     2641 acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa aagagtttca
     2701 tctcgataat cccagcgact actttatcaa ctactgccgc cgctacagcg acatgccgat
     2761 gctggtaatg ctggagcctc gcgacgatgg tagctacgtt cccgggcgca tgatccgcgc
     2821 atctgacctg gtggatggac tgggcgaaag caacaatccg cagtggaaaa ccgtagcagt
     2881 taataccgca ggtgaattgg tagtgccgaa cggtgcgatt ggtttccgct ggggagaaaa
     2941 aggcaaatgg aatctggaat ccattgccgc cggtacggaa accgaattgt cgttaaccct
     3001 gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc tactttggcg gcattgaaaa
     3061 tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg cgccaattgc ccgttaaaaa
     3121 cctgacgtta gtcgatggca acacctgtcc ggtggtcagc gtttatgatt tggtactggc
     3181 gaattacggc ctcgatcgcg ggctggaaga tgaaaacagt gcgaaagatt acgctgaaat
     3241 caaaccgtac accccagcct ggggtgagca aattaccggc gtgccgcgcc agtatattga
     3301 atccatcgct cgtgaatttg ccgatactgc ccataaaacg catgggcgct caatgattat
     3361 cctcggcgct ggcgtcaacc actggtatca catggatatg aactaccgtg ggatgatcaa
     3421 tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc ggctgggcac actatgtcgg
     3481 ccaggaaaaa ctgcgcccac aaaccggctg gttgccactg gcctttgcgc tcgactggaa
     3541 ccgaccaccg cgccaaatga acagcacctc gtttttctac aatcattcca gccaatggcg
     3601 ctatgaaaaa gtctctgctc aggagttact ttcaccgctc gccgatgcca gtaagtacag
     3661 cggtcatctg attgatttca acgttcgcgc cgaacgtatg ggctggctac cttctgcgcc
     3721 gcagctgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg gattatcccc
     3781 cacagaattt accgcccagg cgctgaaatc gggcgattta cgtatggcct gcgaacaacc
     3841 agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgctctaacc tgcttggctc
     3901 ctccggcaaa ggccacgagt atatgcagaa gtatctgctg gggaccgaaa gcgggattca
     3961 gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa gaagtcgagt ggcaaactgc
     4021 ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgcatgt ccagtacctg
     4081 cctgttctcc gatatcgttc tgcccaccgc cacctggtac gaaaaagacg atatgaacac
     4141 ctcggatatg catccgttta ttcatccgct ttctgcggcg gtcgatccgg cgtgggaatc
     4201 acgcagcgac tgggaaatct acaaaggtat cgccaaagca ttttcgcaag tgtgcgtggg
     4261 tcatcttggc aaagaaaccg acgtggtatt acaaccccta ctgcatgact ctccggcaga
     4321 gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc tgattccagg
     4381 caaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacgt atgaacgctt
     4441 tacctcgctc gggccattga tggacaaact tggcaatggc ggtaaaggga tttcgtggaa
     4501 tacccaggat gaaatcgatt tcctcggcaa actcaattac accaagcgtg atggcccggc
     4561 acaggggcgt ccgctgattg acaccgccat tgacgcttca gaagtgattc tggcactggc
     4621 accggaaact aacggtcatg ttgcagtcaa agcgtggcag gcgctgggcg agatcaccgg
     4681 gcgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct ttcgcgatat
     4741 tcaggcgcag ccgcgtaaaa ttatctccag ccccacctgg tccggtctgg aaagcgatca
     4801 cgtatcctat aacgcgggat acaccaacgt tcatgagtta attccgtggc gcacgctgtc
     4861 gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg aaagcctggt
     4921 ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag atgcgccaga taccgccaaa
     4981 cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg gcattcactc
     5041 aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga ttgtctggat
     5101 cagcgaaaca gatgcccgag aactgaccat tgtcgataac gactgggtgg aagtattcaa
     5161 cgccaacggc gcgctgactg cccgcgcggt ggtcagccaa cgtgtaccgc cgggcatgac
     5221 catgatgtat cacgctcagg aacgcattat gaatattcct ggttcggaag taactggcat
     5281 gcgcggcggc attcataact cggtcacccg cgtttgcccg aaaccaacgc atatgattgg
     5341 cggttacgcg cagctggcct ggggctttaa ctactacggc accgtcggat cgaaccgcga
     5401 tgagttcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg aaggtcgcga
     5461 tcaggtacag gaggcgaaaa aatgaaaatc cgttcacaag tcggcatggt gcttaacctc
     5521 gacaaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg gaccgggcgc
     5581 gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat tggttatccg
     5641 aaaaactggg aagatcagga agagtggcaa ggcggctggg tccgcgatgt gaatggcaag
     5701 atccgtccgc gtctgggcag caagatgggc gtaataacca aaatcttcgc caacccggtg
     5761 gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc
     5821 gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cactgattga cggcaaacgg
     5881 atggacaaag tgatctgggg cccaaactgg gaagaactgc tgggcggcga gtttgaaaaa
     5941 cgtgcccgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac
     6001 accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ttgcgtggcg
     6061 acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gattgatcag
     6121 gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc
     6181 aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt atccgcgaat tgagtcaggc
     6241 caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt
     6301 tacgacgccg accgcattga ggaagcggcg agcaccgagc gcgaagttga cctctatgaa
     6361 cgtcagtgcg aagtgttcct cgatccacac gatccctcag tgatcgagga agccctgaaa
     6421 caaggtattc cacaaaacgt gattgaagct gcccagcgtt cgcctgtcta caaaatggcg
     6481 atggactgga aactggcgct accgctgcac cctgaatatc gcaccctgcc aatggtctgg
     6541 tacgttcctc cgctgtcgcc gattcagtcc tacgcagatg cgggcggttt gccgaaaagc
     6601 gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct cgccaatatg
     6661 ttgagtgccg gcgataccgg tccggtactg cgggcgctga aacggatgat ggcgatgcgc
     6721 cactatatgc gttcacaaac cgtggaaggc gttactgata ctcgtgccat cgacgaagta
     6781 ggcctgagcg tcgcccaggt cgaagagatg tatcgttacc tcgccattgc caactatgaa
     6841 gatcgttttg tcatcccgac gagccatcgg gaaatggcgg gcgatgcctt cgcagaacgc
     6901 aacggctgcg gttttacctt tggcgacggt tgtcacggct cggacagcaa attcaacctg
     6961 ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa agcggagggc
     7021 gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggat gagctgttgt
     7081 gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga cgcgccgatg cttacggatt
     7141 tcacccacaa cctgcttaac gcgccgctgc tggataaaca ggccgaatgg tgcgaagtgt
     7201 ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca tgttcatgcc gagtcccgcg
     7261 atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggc ctgcagctgg
     7321 attgtcggga actgcccgat tatctaccgc tgtatctgga gtatttaagc gtactgcctg
     7381 acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat cctcgccctg cttggcggtc
     7441 gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga tgctctgctg caactggcgg
     7501 gaagtatact ttcaagtgac agtgtcacaa aacaagtaaa cagcgaagag cgcgatgaca
     7561 cccgccaggc gcttgatgca gtgtgggaag aggaacaggt gaagtttatt gaagataacg
     7621 ccacggcgtg tgacagctcg ccgcttaatc actatcagcg acgctttagc caggatgtcg
     7681 cgccgcagta tgtcgacatc agtgcgggag gtgggaaatg attcagtatc tgaacgtctt
     7741 tttttacgat atctacccgt acatttgtgc gacggtgttt ttcctcggca gctggctgcg
     7801 ctacgactac gggcagtaca cctggcgcgc ctcctcaagt caaatgctcg ataaacgcgg
     7861 gatggttata tggtcgaatc tgttccatat cggcattttg gggatcttct tcgggcacct
     7921 gttcggcatg ttaacgccgc actggatgta cgcgtggttt ttgccagtgg cagcgaaaca
     7981 actaatggcg atggtgctcg gcggtatttg cggtgtgttg acattgattg gcggcgcagg
     8041 actgctgtgg cgcaggctga caaatcagcg cgtgcgtgca acttccacta cgccggatat
     8101 tatcatcatg agcattttgc tgatccagtg tctgctgggg ctaagtacca taccgttttc
     8161 agcacagtat cctgatggta gcgaaatgat gaagctggtg ggttgggcgc aaagcattgt
     8221 cactttccgt ggtggatcgt cagaaatgct taacggcgta gcgttcgttt tccgcctgca
     8281 tctggtattg ggaatgacca tcttcctgct cttcccgttc accagattgg tgcatgtgtg
     8341 gagcgcgccg tttgagtact ttactcgtcg gtatcagatt gtgcgttcgc ggcgataatt
     8401 ttcaccctgg ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca
     8461 gccacaggag aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctcacaac
     8521 cgtttcgcgg caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa
     8581 tgcagcttat cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacaacgacg
     8641 acagcgatgt gcgcatccgc tactttacgc caacggttga agtgccgatt tgcggtcacg
     8701 cgacagtagc tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgatct
     8761 ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc
     8821 gtatttcgct ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacacgtg
     8881 aggcgattat caacgcgctt catctcactg aggacgatat tctgccaggc ttgccaattc
     8941 aggtggcaac aacaggacac tcaaaagtga tgatcccgtt gaaaccagaa gtggatatcg
     9001 acgccctttc gcctgatctt gctgcgctga gcgctatcag caaacagatt ggctgcaatg
     9061 gtttcttccc gttccagatc cgcccaggca aaaacgaaac cgatggtcgc atgttctcgc
     9121 ctgcgattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat
     9181 ggttggtaca tcacaacgta ttgccccacg atggcaaggt gctgcgtgtt aaaggccatc
     9241 aggggcgcgc attggggcgt gacggcgtga ttgaagtgac ggtgacaatt cgtgataacc
     9301 aaccggaaaa agtcaccatt tcaggtacag cggtgatttt gtttcatgcc gaatgggcaa
     9361 ttgaaatctg aaatcgttgc ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg
     9421 tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct
     9481 ccggatgcgt atcaaacgcc gccatcacca gcgctaactc atccacagta aagccatgtt
     9541 tcgcatcatc cacgcccaga ccaaactgtt cctgcatcac ggcatataac gacaccacat
     9601 ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg
     9661 tcagcgttag cttgccaccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat
     9721 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
     9781 cgcagagatc aaaacggtac atcgactgcc aatgctgatg atgattaaac tgcaacaccc
     9841 aatcatcacc ctcctgcaac aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg
     9901 aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
     9961 tttcctcttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
    10021 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ctccagctcg cgtaacaccc
    10081 gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct
    10141 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa
    10201 acggaatagt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag
    10261 cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt
    10321 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
    10381 gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc
    10441 gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc
    10501 ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc atgatcacca
    10561 gtttcgatga gcaatcccag cggcgtaaca ttatggcagc agcctggtca atgccggtgg
    10621 agtttgaacc gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga
    10681 ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt
    10741 gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt taattgctat ggcattccgg
    10801 tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg
    10861 agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggcgagg
    10921 tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg
    10981 ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttactagc ggcaagcgtg
    11041 ttacggcagg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttgg
    11101 ggcgcgattt cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca
    11161 atgcttatcg aactgtcttt actgttcaga tggcgaataa taacgtcggt aatatctgca
    11221 gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc
    11281 ggcatgattg tgctcctttt gaaagatgtc agtaaaatta acacacattg tgaattttca
    11341 gtcctttcca ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa acaatatgaa
    11401 tacggatttc ttctctgccg tgactctttt cacaaattgc gtaactgtca tgctctggat
    11461 tatttaattc tttcagcgga aatttttcct caaaggcttt atttagccac ccctggtttc
    11521 ctttcaccgc gaataaataa tcacctccct gtttttgtat cttctctgct atatctttct
    11581 ggcaacccat cgcatcagtt gtgatgattt ttcctttaat atccagcatg ttaagaagtt
    11641 ctgggatagc tgtaatctca ttagatttat catccgtctt gatctgtccg atgaccagac
    11701 tgtgcattgt tgagaacgca ctaatgacat gaatcgctcc cctgcggcga ctcttatcat
    11761 aagaatgccg gagcgttttt ccatcaattg caatgacgtc tttatcatct gaagaatgac
    11821 aatcacgcat ccagttaata aagcactcat gaaattttgt agg
//
LOCUS       DAPHDX010000133        11846 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPHDX010000133 DAPHDX010000000
VERSION     DAPHDX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.135"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..548
                     /locus_tag="RH058_003648"
     CDS             <1..548
                     /locus_tag="RH058_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3139180.1"
                     /translation="TAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDH
                     KLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYT
                     IFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVG
                     AADAPDLGNFEANTTFQITYL"
     gene            630..3272
                     /gene="yfcU"
                     /locus_tag="RH058_003649"
     CDS             630..3272
                     /gene="yfcU"
                     /locus_tag="RH058_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDV3139181.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3292..4044
                     /locus_tag="RH058_003650"
     CDS             3292..4044
                     /locus_tag="RH058_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3139182.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKTNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4019..4567
                     /locus_tag="RH058_003651"
     CDS             4019..4567
                     /locus_tag="RH058_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3139183.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4564..5034
                     /locus_tag="RH058_003652"
     CDS             4564..5034
                     /locus_tag="RH058_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3139184.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5031..5555
                     /locus_tag="RH058_003653"
     CDS             5031..5555
                     /locus_tag="RH058_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3139185.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTSFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSAIVTATFEYF"
     gene            5542..6414
                     /locus_tag="RH058_003654"
     CDS             5542..6414
                     /locus_tag="RH058_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV3139186.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6536..7087)
                     /gene="smrB"
                     /locus_tag="RH058_003655"
     CDS             complement(6536..7087)
                     /gene="smrB"
                     /locus_tag="RH058_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV3139187.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7253..8185
                     /gene="prmB"
                     /locus_tag="RH058_003656"
     CDS             7253..8185
                     /gene="prmB"
                     /locus_tag="RH058_003656"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV3139188.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            8220..9305
                     /gene="aroC"
                     /locus_tag="RH058_003657"
     CDS             8220..9305
                     /gene="aroC"
                     /locus_tag="RH058_003657"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV3139189.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9309..10133
                     /gene="mepA"
                     /locus_tag="RH058_003658"
     CDS             9309..10133
                     /gene="mepA"
                     /locus_tag="RH058_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV3139190.1"
                     /translation="MNKTAIALLALLACSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDLRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10133..10942
                     /locus_tag="RH058_003659"
     CDS             10133..10942
                     /locus_tag="RH058_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV3139191.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10942..11490
                     /gene="epmC"
                     /locus_tag="RH058_003660"
     CDS             10942..11490
                     /gene="epmC"
                     /locus_tag="RH058_003660"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV3139192.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11524..11802
                     /locus_tag="RH058_003661"
     CDS             11524..11802
                     /locus_tag="RH058_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV3139193.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2933 a         2889 c         3092 g         2932 t
ORIGIN      
        1 aaacagctat tgctgcggca atggtgatgg gcgttttcac ctctacggca acaatcgctg
       61 cgggtaacaa cggtactgca cgtttttacg gcaccattga agactcagtg tgttctatcg
      121 ttccggacga tcacaaactg gaagttgata tgggtgatat cggtgccgaa aaactgaaaa
      181 ataacggcac caccacgccg aaaagtttcc agattcgtct gcaagattgc gtatttgata
      241 ctcaggaaac aatgaccact acctttactg gtaccgtttc ttctgcaaat agcggcaatt
      301 attacaccat tttcaatacc gataccggtg cggcatttaa caatgttagc ctggcgatcg
      361 gtgactctct gggtacctct tacaaaagcg gcatgggtat tgaccagaaa atagtgaaag
      421 acacttctac caataaaggc aaagcgaagc agacactgaa ctttaaagcc tggctggtcg
      481 gcgcagctga tgcgccagat ctgggtaatt tcgaagcaaa taccaccttc cagattactt
      541 acctgtaagg cataccccca tagcgacact gctatggggg atttaaatgg attttgaccg
      601 aacaagaata aaaacggata gaaacgtgta tgcctgacca ttctcttttt cgattacgga
      661 ttcttccgtg gtgcattgcg ctggcaatgt cagggagtta tagcagtgtc tgggctgaag
      721 acgacattca gtttgattcc cgttttctgg aattaaaagg cgacacaaaa attgatctga
      781 agcgtttttc cagccaggga tatgttgagc ccggaaaata caatttacag gttcaactaa
      841 ataaacagcc attggcggaa gagtacgata tttactggta tgctggtgaa gatgacgcga
      901 gcaaaagcta tgcttgtctg acaccggaac tggtagcgca gtttggttta aaagaagacg
      961 tggcgaaaaa tctgcaatgg agccacgatg ctaaatgcct gaaatccggt caactggaag
     1021 gcatggaaat taaggctgat ttaagccagt ccgcattagt catttcactg ccacaggctt
     1081 acctcgaata tacttatccc gactgggatc cgccttcacg ttgggatgac ggcatctccg
     1141 ggatcgtcgc ggactacagc atcaacgcac aaacccggca cgaagaaaat ggcggtgatg
     1201 atagtaacga gatcagcggc aacgggacgg tcggggttaa cctggggccg tggcgtatgc
     1261 gtgctgactg gcagactaac tatcaacata ctcgcagtaa tgatgacgat gaattcagcg
     1321 gcgatgaaac tcaaaaaaaa tgggagtgga gtcgctacta tgcctggcgg gcgttaccat
     1381 cattaaaagc caaactggcg ctgggcgagg attacctcag atccgatatt tttgatggtt
     1441 ttaactatgt tggtggcagt gtcagtactg acgatcaaat gttgcctccc aatctgcgcg
     1501 gctacgcgcc agacatttcc ggcgtggcac acaccacagc aaaagtgacc gtcagccaga
     1561 tggggcgtgt gatttacgaa acgcaggttc cggctggacc gtttcgtatt caggatcttg
     1621 gtgattctgt ctccggtacg ttgcatattc gcattgaaga acagaacggc caggtgcagg
     1681 aatatgacat cagcaccgcc tcgatgccat acctcactcg cccaggtcag gttcgctata
     1741 agatcatgat gggccgtccg caagagtggg gacaccatgt cgagggtgaa tttttttctg
     1801 gtgctgaagc ttcctggggg atcgctaacg gctggtcgtt atatggcggc gcactgggag
     1861 atgaaaacta tcagtctgcg gcgcttggcg tcggtcgcga tttgtctaca ttcggcgcgg
     1921 tcgcgtttga tgttactcac tcgcacacca aactggataa agacaccgct tatggcaaag
     1981 gttcgctgga cggtaactcc ttccgtgtga gttattccaa agactttgac cagctcaaca
     2041 gccgcgttac ttttgctgga tatcgcttct cggaagagaa ctttatgacc atgagcgagt
     2101 atctggatgc cagtgacagc ggaatggtac gcacgggcaa cgacaaagag atgtacaccg
     2161 ccacttataa ccagaacttc cgcgatgcgg gtgtttcggt ttatctcaac tatacccgcc
     2221 atacctactg ggatcgcgag gagcagacaa actacaacat catgctctcg cactatttca
     2281 atatgggcag tattcgtaat gtcagcatct cgatgactgg ctaccgttac gagtatgaca
     2341 accaggccga caaaggcatg tacatttcgc tcagtatgcc gtggggcgac aacagtaccg
     2401 ttagctataa cggtaactat ggcagtggga cggacagcag tcaggtcggt tatttcagcc
     2461 gtgtcgatga cgcgactcac tatcagttga acgtcggcac cagtgacaaa cacaccagcg
     2521 ttgacggcta ttacagccat gatggttcgc tggcgcaggt tgacctcagt gcgaactacc
     2581 atgaagggca atacacctct gcgggcttgt cgttacaggg cggcgcaacg cttactgccc
     2641 acggtggcgc acttcaccgt acccagaata tgggcgggac acgcttgttg attgatgccg
     2701 atggcgttgc cgatgttccg gtagaaggta acggtgctgc tgtttatacc aatatgtttg
     2761 gtaaagccgt cgtttctgac gtcaataact attaccgcaa tcaggcgtat atcgacctca
     2821 acaaactgcc ggaaaacgcc gaagcaaccc agtcggtggt acaagccacg ctaactgaag
     2881 gggcgattgg ctaccgcaaa tttgccgtca tcagtggtca aaaagcgatg gcggtgctgc
     2941 gtttacaaga cggcagccat cctccgtttg gcgcagaagt aaaaaatgat aacgagcaga
     3001 cagtgggcct tgtcgatgat gacggcaatg tttatctggc tggggtgaaa cctggcgaac
     3061 acatgagtgt gttctggagt ggtgttgcgc attgcgatat caacctgccg gacccgctgc
     3121 ctgccgatct gtttaacggc ttgttactgc catgccagca taaaggcaac gtcgcgccgg
     3181 ttgttcctga tgacataaag cctgtcattc aggagcagac gcaacaggtg acacccacca
     3241 atccccctgt ttctgtttca gctaaccaat aacgtgatta aggaatgatc catgtctgat
     3301 ttactttgct ctgccaaatt gggtgctacg acgctagcgt tactgcttag cgctgccagt
     3361 ttgtctgccc aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac
     3421 aaatcaatca gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc
     3481 tggatcgaag atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg
     3541 caacgcatcg attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac
     3601 aaattgcctg ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa
     3661 acaaacaaag ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga
     3721 ccgaaggcgc tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc
     3781 cgcaacggtc aggaatttac cgtcaataac ccgacaccgt attacgtcat tttcagtaat
     3841 gccagcacac agaaaaatgg caatccggca gccggtttct cgccaatagt gatgtcgccg
     3901 aaaacctctg caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac
     3961 gactatggcg ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat
     4021 gaagagcaga gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt
     4081 ctgggcggta actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag
     4141 taagtcagta agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa
     4201 tggtgcggca gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa
     4261 acgtcccatt gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca
     4321 gatgcaggcg acaacggtcg tgattaacgg tgaaacagtt atcagcacgg ggattcctgg
     4381 gtttggtatt cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc
     4441 atggttgcca tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca
     4501 gagtggtaca acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta
     4561 ccaatgaaac ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac
     4621 gtctcttttc acggttattt agtgcagcca cctaactgca cgattagcaa cgctcaaacc
     4681 attgaaataa cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa
     4741 accgtgccgt attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc
     4801 ttgagctgga gtggcacacc aagcgatttt gataatgctg cagtaagcag caatatcact
     4861 ggtttaggca ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttgtg
     4921 gtgaatgaaa ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt
     4981 ttgccagagg cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa
     5041 agcgtacgtt gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag
     5101 gagataattt gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg
     5161 gtagccagtt ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg
     5221 ggcagtcaga gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacatcgt
     5281 ttaatgtcaa agtaacgttg acaggaacag aagatagtgc tttacccggc tttctggcct
     5341 ttgactcctc gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat
     5401 cggtgccaat caacaataca acaggcgtca cgctcccact gaatcaggga aataacagcc
     5461 taaattttaa tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta
     5521 gtgccatagt gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc
     5581 gcgagcctaa tgctgctcgt tctgagtgtt gtcggcgcat ctaaaagtta tgctggagat
     5641 aaacttatga gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc
     5701 ggtgctgtag cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttgcatat
     5761 gggaaatatg attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg
     5821 tcgggttcag ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc
     5881 cctggcatag atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca
     5941 tctgatgatt atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga
     6001 tttggaagct atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcgct
     6061 tcatacgata tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag
     6121 tccacggggt caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc
     6181 tgtgatggca gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca
     6241 gaattgttaa cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact
     6301 cgtcatccgg taacggataa agtacttcac gatattgtgg tgaatgtgaa taccagtagt
     6361 gggcaaccga ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta
     6421 gtattgctct gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca
     6481 catatttgta ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg
     6541 gcaactccgg cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc
     6601 cgtactcttt cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg
     6661 gtgtttgctg cttcaatatg tgcttcccgt gaccatgcat cacgcaggca caaaacacat
     6721 gttcgcggcg gcaggcagca atcaatgcgc ctaattcctg cttggcttgt agctgcgtca
     6781 gaccgtgcaa atccaaaaac aactccggtg aataatcgcc gcggcgcagt ttcttcgcct
     6841 caaaatggct gacatccggg cgaacatatt tcaccggacc ttcggtattc aataacggct
     6901 gaaactcatc ggaaaaataa tggctggcat cggcctgctc ctgaatcaac cgtttcaccg
     6961 gcacttcgct aattttctta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc
     7021 gagtccccgc catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct
     7081 ttttcattta tctgtctcaa tttctaattt tcctacagtt tacccgactc gtagcgcaat
     7141 cgatcgtttt aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc
     7201 ttcatggcaa actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa
     7261 aattttcgtt gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc
     7321 ggtgagccgc ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga
     7381 tgaagccgta cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat
     7441 gcgcaccgcg cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg
     7501 cgtcaacgag cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga
     7561 attttacgtc gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa
     7621 taaatttgcc ggactcatca gcaagcaacc gcagcacatt ttagatatgt gtaccggtag
     7681 cggctgcatc gccattgcct gtgcttatgc cttcccggat gcggaagtcg acgcggtgga
     7741 tatctctccc gacgcgctgg cggttgctga acagaacatc gaagaacacg gtctgatcca
     7801 caacgtcatt ccgattcgtt ccgatctgtt ccgcgacttg ccgaaagtgc agtacgacct
     7861 gattgtcact aacccgccgt atgtcgatgc ggaagatatg tccgacctgc caaacgaata
     7921 ccgtcacgag ccggaactag gcctggcatc tggcaccgac ggcctgaaac tgacgcgtcg
     7981 cattctcgga aacgcggcag attaccttgc ggatgatggc gtgttgattt gtgaagtcgg
     8041 caacagcatg gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt
     8101 tgataacggc ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga
     8161 acatttcgcg atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga
     8221 tggctggaaa cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcacgggc
     8281 tggcgctcgg ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc
     8341 tgcaacatga ccttgatcgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg
     8401 agccggatca ggtcaaaata ctctccggtg tttttgaagg cgttactact ggcaccagca
     8461 ttggcttgtt gatcgaaaat accgaccagc gttctcagga ttacagcgca attaaagacg
     8521 tcttccgtcc aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc
     8581 gcggcggtgg acgttcttcc gcccgcgaaa ccgccatgcg cgttgcagca ggggcgattg
     8641 ccaaaaaata tctcgccgag aaatttggta ttgaaatccg cggctgcctg acccagatgg
     8701 gagacattcc gctggaaatc aaagactggt cgcaggttga acaaaatccg ttcttctgcc
     8761 cggacccgga taaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg
     8821 actccatcgg cgcgaaagtc accgttgttg ccagtggcgt tcctgccgga cttggcgagc
     8881 cggtctttga ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga
     8941 aaggcgtgga aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg
     9001 acgaaatcac caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca
     9061 gcagcgggca gcaaatcatt gcccatatgg cgctgaaacc gacctccagc attaccgtgc
     9121 cggggcgcac tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg
     9181 atccttgtgt cgggatccgc gcggtgccga tcgctgaagc gatgctggcg atcgttttaa
     9241 tggatcacct gctacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct
     9301 ggtaaaaaat gaacaaaacc gcgattgcgc tgctggctct gcttgcctgt agcgctagcc
     9361 tggcagcgac gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag
     9421 gcagtttttc taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt
     9481 atcaggtcat gcgtaccgat ctgcgtcgct atttcggtca cccggatctg gtgatgttta
     9541 tccagcgtct gagtagccag gtgagcaatc tgggcatggg cacggtgctg attggcgata
     9601 tggggatgcc cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg
     9661 atgtcgatat ttttctgcaa ctgccgaaaa cacgctggac ctccgcgcaa cttttgcgcc
     9721 cgcaagcact ggacttagtt tcccgcgacg gtaaacacgt tgttccgacg ctgtggaagc
     9781 cagaaatttt cagcttgatc aaactcgccg cccaggacaa agacgtcaca cgcatttttg
     9841 ttaatccggc gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc
     9901 gcaaagtgcg accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg
     9961 ccgatagtct ggagtgtgaa gatcaacctt taccgccacc aggcgatggt tgcggggcag
    10021 aactgcaaag ctggtttgaa cctccaaaac cgggaacaac aaagcctgag aagaagactc
    10081 cgcctccgtt gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac
    10141 gtttaatagc ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat
    10201 gctggcggga tttatcgact cgattgccgg tggcggtggg ttactcacta ttccggcatt
    10261 gatggctgcg ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg
    10321 cggctctatt tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca
    10381 gaaactcaat atcgccatga cctttgtcgg ctcaatgagc ggcgcactgc tggtgcaata
    10441 cgttcaggct gatgtcttac ggcagatttt gcccattctg gtaatttgta tcggcctcta
    10501 tttcttgctg atgccaaagc tgggtgaaga agatcgccag cgtcggatgt acggtttacc
    10561 cttcgcgtta attgccggtg gctgcgtcgg tttttatgac ggattctttg gtccggcagc
    10621 cggatcgttt tacgcactgg cctttgttac gctgtgcgga ttcaacctcg ccaaagccac
    10681 ggcgcacgcc aaattactca acgcaacgtc aaacatcggc ggtttactgc tgtttattct
    10741 cggtggcaaa gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc
    10801 gcgcatgggt tcacgactgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt
    10861 tatcgtctcg gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct
    10921 ccactggttg gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt
    10981 tttaatagct gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg
    11041 atctatcttc ctgctgatgc ggaagtaccg tataaccgaa tcgtctttgc tcatggcttt
    11101 tatgccagcg caattcatga gatttcgcac tggtgtattg ccgggaaagc gcgccgtgag
    11161 ctggttgatt tcggctactg gtattgcccg gacggacgcg atgcccaaac gcaaagccag
    11221 tttgaagatg ttgaagtgaa gccgcaggcg ttcgactggt tgttctgtgt ggcggcggga
    11281 tatccgttta acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgtggtg
    11341 tttcaacggc gggtacatgc gcaggtgatg gattacctag caaacggcat tcctgaacgc
    11401 ccggcacgct tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa
    11461 cagttcccgt ggccggaagc gctcaactaa agccagcgcc acattttaca atgaggaaag
    11521 aagatgatcg cggagtttga atcacgcatt ctggcattaa tcgacggtat ggttgaccat
    11581 gccagtgatg atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc
    11641 gcagaactgg aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag
    11701 agtctggaaa aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc
    11761 gatatgtggg aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca
    11821 ccatgcctga tgcgacgctt atcgcg
//
LOCUS       DAPHDX010000134        11700 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPHDX010000134 DAPHDX010000000
VERSION     DAPHDX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.136"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..617
                     /locus_tag="RH058_003662"
     CDS             15..617
                     /locus_tag="RH058_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV3139194.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(619..1140)
                     /gene="ruvC"
                     /locus_tag="RH058_003663"
     CDS             complement(619..1140)
                     /gene="ruvC"
                     /locus_tag="RH058_003663"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV3139195.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1175..1915)
                     /locus_tag="RH058_003664"
     CDS             complement(1175..1915)
                     /locus_tag="RH058_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3139196.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1944..2396)
                     /gene="nudB"
                     /locus_tag="RH058_003665"
     CDS             complement(1944..2396)
                     /gene="nudB"
                     /locus_tag="RH058_003665"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV3139197.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAVREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2389..4161)
                     /gene="aspS"
                     /locus_tag="RH058_003666"
     CDS             complement(2389..4161)
                     /gene="aspS"
                     /locus_tag="RH058_003666"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV3139198.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4471..5037
                     /locus_tag="RH058_003667"
     CDS             4471..5037
                     /locus_tag="RH058_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3139199.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5034..5852
                     /locus_tag="RH058_003668"
     CDS             5034..5852
                     /locus_tag="RH058_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV3139200.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLVQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5905..6300
                     /locus_tag="RH058_003669"
     CDS             5905..6300
                     /locus_tag="RH058_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV3139201.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6341..7084
                     /gene="cmoA"
                     /locus_tag="RH058_003670"
     CDS             6341..7084
                     /gene="cmoA"
                     /locus_tag="RH058_003670"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV3139202.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7081..8052
                     /gene="cmoB"
                     /locus_tag="RH058_003671"
     CDS             7081..8052
                     /gene="cmoB"
                     /locus_tag="RH058_003671"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV3139203.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8217..10646)
                     /gene="torZ"
                     /locus_tag="RH058_003672"
     CDS             complement(8217..10646)
                     /gene="torZ"
                     /locus_tag="RH058_003672"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV3139204.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYCTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(10671..>11700)
                     /locus_tag="RH058_003673"
     CDS             complement(10671..>11700)
                     /locus_tag="RH058_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDV3139205.1"
                     /translation="LAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAEC
                     ADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKELKATDS
                     ATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHE
                     LESQAATSVTNGAHIYPFKTSRIGELATVNPGADLTVVDASGKQPIVLLQGYQMQGSE
                     NTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKP
                     LWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYN
                     AKDITEKQ"
BASE COUNT         2655 a         3121 c         2872 g         3052 t
ORIGIN      
        1 aatggaatga caacgtgaat attaattacc ctgctgaata tgaaattgga gatatcgtct
       61 ttacatgtat aggtgctgcc ttatttggtc aaatatcagc tgcatcaaat tgctggagta
      121 atcacgtcgg gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg
      181 ttcccctttc aaccatcacc acgctatctc gttttattaa acgttcgtct aatcaacgct
      241 atgctataaa gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac
      301 aggttccttc ccggctacgc aaactttacc acacgggttt taaatacgaa tcttcccgcc
      361 agttctgttc aaaatttgtc tttgatattt ataaagaggc gctatgtatt ccggtgggtg
      421 aaatagagac gtttggacaa ctgttaaata gcaatccgaa tgcaaaactc actttctgga
      481 aattctggtt cttaggttct attccgtggg agcgtaaaac cgtcacgcca gccagtttgt
      541 ggcatcatcc gggtttggtg ttgattcacg cggagggagt tgaaacgcct cagcctgaac
      601 tgaccgaggc ggtataactt aacgcagtcg ccctctcgcc aggttcagcc gcgattcgct
      661 catctgcatc gcattctgac tgacgtgaca atgggtaatc gcgatagcta acgcatcggc
      721 ggcatccgcc tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg
      781 gcttttttcg gcactaccaa tacctaccac cgtttgcttt acctgacgtg ccgcgtattc
      841 aaataccggc aattcctgat tcaccgccgc aacaatcgcc acgccgcgcg cctggcccag
      901 tttcagggct gagtcagcgt tcttcgccat aaagacttgt tcaatggcga aataatcagg
      961 ctggaactgg gtgatgattt ccgtcacgcc cgcatagatg agcttcagac gagacggtaa
     1021 atcatccact ttggtgcgta tgcatccgct acccaggtag gacagttgcc tgcctacctg
     1081 gcgaattacg ccatagccgg tcacgcgcga acccggatca atgccgagaa taatagccat
     1141 cacgcgtctc cgttttgctg tttagcaggc ctcatcagag agtcgctgcg acctcatcag
     1201 agatttcacc gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca
     1261 gacgcatcag tttcggtgcg gtttctgcat ccatatcagc tttggtggac gggatcatgg
     1321 aaacttccgc gctgtctgct ttcagacctg ccgcttccag agcgtcgcgc actttaccca
     1381 tttcttccca tgcagtgtag acatcaatcg cgccgtcatc ataggtcaca acgtcttcag
     1441 cacctgcttc cagagctgct tccatgatgg tgtcttcatc gcctttctcg aaggagatca
     1501 cgcctttttt gctgaacaga taggctacgg aaccatctgt accgaggtta ccgccacatt
     1561 tgctgaatgc atgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa
     1621 tcatgattgc cgtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgtttgcat
     1681 catcatcacc gcccacacca cgtgcaattg cgcggttcag tgtgtcgcgg gtcatgttgt
     1741 tagacagtgc tttatcaatt gctgcacgca gacgcgggtt agcgtccgga tcgccaccgc
     1801 ccagcttagc cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgctttg
     1861 catcctgcgc agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa
     1921 tatctccaga tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc
     1981 cggttgctcc aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc
     2041 agatgttcag tgaaaacgat ctgccgctcg tgtgggagcg caagacagaa ccatgattcc
     2101 gtattacgcg tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct
     2161 accgtgcgct gacagtcaat taaggtcagt tgttcagcga caacatcaat ggtgacctct
     2221 tcctttactt cgcgcacggc agcttgcggc gcggtttccc cctcttccac gctgccggtt
     2281 accgactgcc agaaatcggg atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct
     2341 tgtgcgtaga tgaccactaa gatcgaaacg ggacgcttat aagccatatc agttattctc
     2401 agccttcttc acaacctgaa tgctcagctc agccagtgca gtcgggttag caaagctcgg
     2461 tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcaataa cgtcacggat
     2521 attgtcggtg ccggtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg
     2581 cggcggagta ccgtatttca gagcgtcgag caggaagccg aatttctcgc gctgttcctc
     2641 ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgtac
     2701 tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt
     2761 ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg
     2821 cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga taacccacag
     2881 cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc
     2941 gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc
     3001 ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga acttcgctac
     3061 cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc
     3121 tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga
     3181 tgcgccgccc ggaacgcgca gagcggcaac gcgacctttc ggatcgttcg ccggacctgc
     3241 aaatactgca aactcaacag atttcagcag atcagcaacg tcggtcagtt ccatcgggtt
     3301 acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg tcattaccgg
     3361 gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca gcgcttccat
     3421 cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc
     3481 aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg
     3541 gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta
     3601 gaatttacct ttgtgcacac gagaaggcac caggtagtca cgcgcgcctt ccggcgtggc
     3661 tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa aacggcgcac
     3721 caggctggtg attttagcgc gggttttcag gcgctgcgcc atttccgggc gacgcaggtc
     3781 gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag
     3841 aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcaattt cgccggtcgc
     3901 catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtaa cctgaatgca
     3961 gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa
     4021 tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg
     4081 acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg
     4141 tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg
     4201 ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg
     4261 ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgga acgcctcgtt
     4321 accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc
     4381 gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg
     4441 aggcacgatt ttgttttttc aggagtcagc atgcttgaac ttaatgctaa aaccactgcg
     4501 ttggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc
     4561 gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc
     4621 gtgtttctgg tgcgcgttgg ctggtctgcc gatcacgccg aagcattaaa acagccggtt
     4681 gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta
     4741 ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg
     4801 gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg
     4861 accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg
     4921 attgccgaag acgcctgtag tgccgccagc gccgagcagc acaataacag catcaaccat
     4981 atctacccac gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt
     5041 acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttgggaatc accagccttg
     5101 aagagtatgc ccgccacttt aactgcgtgg agggcaacac cacgctttac gccctgccga
     5161 aacccgaggt tgtcctgcgc tggcgtgaac agaccacaga tgacttccgc ttctgtttta
     5221 agtttccggc gaccatttcg catcaggcag cattacgaca ttgcgatgat ttagtgactg
     5281 aatttttgac ccgcatgtca ccgttggctc cgcgcattgg gcaatactgg ctgcaactgc
     5341 ctgccacatt cggcccacgg gagctgcctg cgctttggca ttttctcgat tctctccctg
     5401 gcgaatttaa ttatggtgtg gaagtccgcc atccacagtt tttcgccaaa ggggaagagg
     5461 aacaaacgct taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc
     5521 gcccggttca tgcagcacgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac
     5581 ctaaagttcc ggtacatgct gtactgacgg cgacaaatcc gctgatccgt tttatcggta
     5641 gtgatgatat gacgcaaaac cgggaattat ttcaggtctg gttacaaaaa ttagtgcagt
     5701 ggcatcagac cactacgcct tatctttttt tacatacgcc agacattgcc caggccccgg
     5761 aactggtaca taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc
     5821 cggctattcc acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca
     5881 tcggccattt taaagggagt ttgtatggta agcgcgctgt atgccgtttt aagtgcgttg
     5941 ttattaatga agttctcttt tgatgtcgtt cgcttgcgtt tgcagtaccg cgttgcctat
     6001 ggtgacggtg gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa
     6061 tatattccca tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc agaaacctgg
     6121 atggtgcata tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt
     6181 catcaccgtc tgttccgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg
     6241 ctgatggtgc tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag
     6301 cgcacaatac gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct
     6361 attttctgcc cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt
     6421 cttcccggat atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg
     6481 tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct
     6541 gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc
     6601 catcgacaac tccccggcga tgattgaacg ctgccgtcgt catattgacg cctataaagc
     6661 ccctacgcca gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc
     6721 gatggtggtg ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcattact
     6781 ggataaaatt tatcaaggac tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag
     6841 tttcgaagat gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc
     6901 caacggttac agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct
     6961 gaccgattcc gtggaaaccc ataaagcacg cctgcataaa gccggttttg agcatagcga
     7021 gctgtggttc cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc
     7081 atgatcgact ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctc
     7141 gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag
     7201 cagtggtcca acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg
     7261 catagcgtaa ccgccgaaag cgaagagcca ctgagcaccg ggcaaattaa acgcattgaa
     7321 acgcttatgc ggaacctgat gccatggcgc aaagggccgt tctcactgta tggcgtcaac
     7381 atcgataccg aatggcgttc cgactggaaa tgggatcgtg ttctgcccca tctttctgat
     7441 ttaaccggac gcaccattct ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg
     7501 attggcgcag gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt tctgtgccag
     7561 tttgaagcgg tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt
     7621 attgaacagc ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttac
     7681 catcgccgtt cgccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt
     7741 gaactggtgc tggaaacgct ggttattgat ggcgatgaga acacggtgct ggtaccaggc
     7801 gatcgttacg cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac
     7861 tggctgaaga agtgtggttt tgtagatatc cgcattgtgg atgtgtgcgt taccactact
     7921 gaagagcagc gacgcactga atggatggtt accgaatctc tgtccgattt cctcgaccca
     7981 catgatccga gtaagacagt tgaaggttat cctgcgccta aacgcgcggt gctgattgcg
     8041 cgcaagccgt aaaggtctgg tattactgcc ggatgcggct tgaatgcctt atccggccta
     8101 caaacccttg ctaattcaat ctattgcagg gactatgtag gcctgataag catagcgcat
     8161 caggcagttt tacgtttgca taacctcagc gcccgtttcc gggcgctatt cacttcttat
     8221 gcctgaatag ctggctgatc aaacgccgtt aacttcggcg cattgcccgt atatttttcg
     8281 atatacacca gcgcagagtt accggcacag gcatttgcca gctgcgagct ggggatatcc
     8341 gccgttaaca cgttcgcact gccgttttta cacaagccat tttccagatc tggccatgca
     8401 ccttcatgca cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaga
     8461 atctgaccac gtttgttcca cacacgcacc agatcaccat tcgcaatacc aaagcgagca
     8521 gcatcttcgg tgtgaatagt gattggttca cgatctgcaa ccgcatattt tttacgcagt
     8581 tccgcatagt taagctgact atgtaaacgg tgtgccggat gtgcggtcag aagctgcaac
     8641 tgcttctcgt cggcggtacc cttccactca tcaggcgcaa gccaggttgg gtgtgccggg
     8701 caatccttat agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc
     8761 gtacccagcg catttttcac cggatcggcg cggaaatcag cataacgaac gtactgttcg
     8821 ttcttctcgc tgcggcgcat ttcgatcagt ttattttgct gccagaaggc attaaacatc
     8881 ggcatagtca cgcgttgcgc acgagcacct ttctgagcgg catcatagaa aaatttcagc
     8941 cacgccattt catctttacc ttcggtatag atctcttttc cgccaggttt cagtaattca
     9001 gcaagatcgg caaatacgtc aaaatcgtta cgcgcttcaa attgcggagc aacagcctgc
     9061 ttcatcggca caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca
     9121 aacgatgtgg tgatcggtaa tacgatatct gcatgtttag cagctgcggt acagtagcat
     9181 tcagaaacga cgatcatctc cggtttctgc catgctttga tcagacgatt agtatcctgg
     9241 tgatgggtaa agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt
     9301 tctttaccgt tatgctgata tttgccacca ggattttcca gcgcatcgac gatacgggca
     9361 acaggaatcg ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc
     9421 gcagacattt ccggcagcac gccgccaaca cgcgtcggat tgccaccgtt ggagtaatga
     9481 taagagaaac caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc
     9541 accagcatcc agtgtttctg ttcaccatat tgctggcgct gaattcccca gccagccatc
     9601 agcattgtgc gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg
     9661 ccagtaattt ccgccgccca ggccgcgctc ttcggagtat tatcgctctt acctgtcaga
     9721 tactcttcaa actgcggata cccggtggtg tatttttcaa ggaacacttt atcgtgttta
     9781 ccttgtgtca tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc
     9841 ggcgcgatcc aggtggcatt atcaccaaag aactcgatgg tttcggagcg gataggatca
     9901 atggcaatca ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca
     9961 tcggtactgc tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca
    10021 gcctggctgt tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa
    10081 ccgaccacat gcggcatgat cacctgtgct gccccggttg aataatcgcc gctatgcccg
    10141 gaataaccgc ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg
    10201 ccagaagagc gccagccgta tgatccggca aaaatagccg atggtccgtt agctttgcga
    10261 atgcgatcgt gttgctcatg aatcagtttt aatgcttgct cccagctcac ctgtacataa
    10321 gtatcttcgc cacgaccttt cgccggttgc agtggattat cgagatagct ttttcttacc
    10381 atcggatgct gaatacgtgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag
    10441 ttcggtatgg ttttcgccag cgcgcctgtc gaagaaacaa tcttgccgtc cttcacttct
    10501 acgctcatcg ctccccaacg gcccgcggtg aggattttac cgcccttctc ttctgcccat
    10561 gcgggtagcg gtgctgccga tgtcaccacc agcgccccag cggcaatacc gctgtgttta
    10621 ataaattcac gtcttgttaa tgtcataact tcctccctga tcaacgagga tcactgtttc
    10681 tcggtaatat ctttggcgtt gtactggaag taccgcgtca aaatgtccag ttcgttttcg
    10741 ctcatgctgg ttcgcgcacc cattcctttg gcaatggacg gccatgcatt gacggtgtaa
    10801 tggtcagcgg caataggcgc atggcaacca gcgcaatagg tatcgtcaag tttttcagcg
    10861 tattgccata gcggtttacg gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac
    10921 gcctgacgcc attgattgcc gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc
    10981 ttgatacctt cttcacttaa tgtggctagc gccagccgtt gacctgccgc caggtagagc
    11041 gtgttttcac tgccctgcat ttgataaccc tgcaacagaa cgatcggctg tttgccactg
    11101 gcatcaacga cggtgagatc ggcaccagga ttcacggtag ccagctcgcc tatgcgagaa
    11161 gttttgaaag gataaatatg tgcgccatta gtcactgaag tagcggcctg actttccagc
    11221 tcatgcgccg cgttgtcatc catttttatt tctggcggaa aatgggcaat gcctttatga
    11281 caatcgatac aggtttcgcc gcccttttgt gctttgttat gcattttctg cgcagattca
    11341 ctttgcgagg caatatccat ggcatcaaaa gaatggcaac tacggcacgt tgcagagtca
    11401 gttgctttta attctttcca tactgtttcg gccatttcct ggcgatgagt ttcgaactta
    11461 tcgtcactgt ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat
    11521 ttagcaaata aataatccat ccctgacttt ggaatatggc aatcggcaca ttccgcacgt
    11581 atccctttct ggttcgaaaa gtggacagtt ccctgatatt cctcaaaagg tttactcatc
    11641 gagtggcagg aaaggcaaaa tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc
//
LOCUS       DAPHDX010000135        11381 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPHDX010000135 DAPHDX010000000
VERSION     DAPHDX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.137"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            157..516
                     /locus_tag="RH058_003674"
     CDS             157..516
                     /locus_tag="RH058_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV3139206.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(636..815)
                     /locus_tag="RH058_003675"
     CDS             complement(636..815)
                     /locus_tag="RH058_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV3139207.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            889..2250
                     /gene="pabB"
                     /locus_tag="RH058_003676"
     CDS             889..2250
                     /gene="pabB"
                     /locus_tag="RH058_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV3139208.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2254..2832
                     /locus_tag="RH058_003677"
     CDS             2254..2832
                     /locus_tag="RH058_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV3139209.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3016..4380
                     /gene="sdaA"
                     /locus_tag="RH058_003678"
     CDS             3016..4380
                     /gene="sdaA"
                     /locus_tag="RH058_003678"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV3139210.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4511..6109
                     /locus_tag="RH058_003679"
     CDS             4511..6109
                     /locus_tag="RH058_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3139211.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSANPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6113..7669)
                     /gene="yoaE"
                     /locus_tag="RH058_003680"
     CDS             complement(6113..7669)
                     /gene="yoaE"
                     /locus_tag="RH058_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV3139212.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            8131..9102
                     /gene="manX"
                     /locus_tag="RH058_003681"
     CDS             8131..9102
                     /gene="manX"
                     /locus_tag="RH058_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV3139213.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9165..9965
                     /gene="manY"
                     /locus_tag="RH058_003682"
     CDS             9165..9965
                     /gene="manY"
                     /locus_tag="RH058_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV3139214.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9978..10829
                     /gene="manZ"
                     /locus_tag="RH058_003683"
     CDS             9978..10829
                     /gene="manZ"
                     /locus_tag="RH058_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV3139215.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10884..11342
                     /locus_tag="RH058_003684"
     CDS             10884..11342
                     /locus_tag="RH058_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV3139216.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2782 a         2869 c         2871 g         2859 t
ORIGIN      
        1 ttttctttct tagctgaata atctggttat agcgtgagga ctgaccgtta aatttctcca
       61 tcttactgga taacacacca tgagctgcta cctttaaaac acctgaatgt caacaggtta
      121 actcgtgcca tatcgttata atccctttga tattagatgc aaattaaggt catatatagt
      181 cttatcgata acatggttaa ttttaaggat aagaatatgc ctgctgtaat agataaagcc
      241 ctggatttca ttggtgccat ggatgtatca gcgccaacac caagttcgat gaatgaaagc
      301 acggcgaagg gaatctttaa atatttaaaa gaactgggag tacccgcgag tgccgctgat
      361 attacggcgc gagctgacca ggaaggctgg aaccctgggt tcacggaaaa aatggttgga
      421 tgggcaaaaa aaatggagac aggtgaacgc tctgtgatta aaaatcctga atacttttca
      481 acatatatgc aggaagaact aaaagcactg gtctgagtta aatttatatc agcataaatg
      541 ggtcaggacg cttttaatca catataaaaa gcgtcctttt tctcacaatc aaatgatcat
      601 cggcttctat aacgaaatcg atacggatta gtgtgttact cgtcttcatc ctcaaaacgc
      661 gccacgattc gctcgccgga gtggttggca cgcagctctt ctgccaccag cgcgattgcc
      721 tggccgctgc tcatcccttg tgccatcagt tcctggatcc gctcgacagc tttttgctgc
      781 tgttcatggg tgagtgaagg taaacctgca aacattgtta actcctgcta aattgttggc
      841 gctaattatt tcatgctacc cggcacatag ccagtagagt caggactgat gaagacgtta
      901 tctcccgctg tgattacttt accctggcgt caggacgccg ctgaatttta tttctcccgc
      961 ttaagccacc tgccgtgggc gatgctttta cactccggct atgccgatca tccgtatagc
     1021 cgctttgata ttgtggtcgc cgatccgatt tgcactttaa ccactttcgg taaagaaacc
     1081 gttgttagtg aaagcgaaaa acgcacaacg accactgatg acccgctaca ggtgctccag
     1141 caggtgctgg atcgcgcaga cattcgccca acgcataacg aagatttgcc atttcagggc
     1201 ggcgcactgg ggttgtttgg ctacgatctg ggccgccgtt ttgagtcact gccagaaatt
     1261 gcggaacaag atatcgttct gccggatatg gcagtgggta tctacgattg ggcgctcatt
     1321 gtcgaccacc agcgtcatac agtttctttg ctgagtcata atgatgtcaa tgcccgtcgg
     1381 gcctggctgg aaagccagca attctcgccg caggaagatt tcacgctcac ttccgactgg
     1441 caatccaata tgacccgcga gcagtacggc gaaaaatttc gccaggtaca ggaatatctg
     1501 cacagcggtg attgctatca ggtgaatctc gcccagcgtt ttcatgcgac ctattctggc
     1561 gatgaatggc aggcattcct tcagcttaat caggccaacc gcgcgccatt tagcgctttt
     1621 ttacgtcttg aacagggtgc aattttaagc ctttcgccag agcggtttat tctttgtgat
     1681 aatagtgaaa tccagacccg cccgattaaa ggcacgctac cacgcctgcc cgatcctcag
     1741 gaagatagca aacaagcaga aaaactggcg aactcagcga aagatcgtgc cgaaaatctg
     1801 atgattgtcg atttaatgcg taatgatatc ggtcgtgttg ccgtagccgg ttcggtaaaa
     1861 gtaccagagc tcttcgtggt ggaacccttc cctgccgtgc atcatctggt cagcactata
     1921 acggcgcgac taccagaaca gttacacgcc agcgatctgc tgcgcgcagc ttttcctggt
     1981 ggctcaataa ccggggctcc gaaagtacgg gctatggaaa ttatcgacga actggaaccg
     2041 cagcgacgta atgcctggtg cggcagcatt ggctatttga gcttttgcgg caacatggat
     2101 accagcatta ctatccgcac gctgactgcc attaacggac aaatatactg ctctgcgggc
     2161 ggtggaattg tcgccgatag ccaggaagaa gcggaatatc aggaaacttt tgataaagtt
     2221 aataagatat tacgccaact ggagaagtaa gacgtggaat accgtagcct gacgcttgat
     2281 gattttttat ctcgctttca acttttgcgc ccgcaaatta accgggaaac cctaaatcat
     2341 cgtcaggctg ccgtgttaat ccccatcgtc cgtcgaccgc aaccggggtt gttgctgact
     2401 cagcgttcaa ttcatctgcg taaacacgct ggacaagtgg cattccctgg aggtgcagtc
     2461 gatgacacgg acgcatcagt tatcgccgcc gcgctgcgcg aagctgaaga agaggtcgct
     2521 ataccgcctt ccgccgttga agttatcggc gtgctgccgc ccgtcgatag cgtaactggc
     2581 tatcaggtaa ccccagtggt cggcattatc ccgcccgatc tgccgtatcg cgccagtgaa
     2641 gatgaagtct cggcggtgtt tgaaatgccg ctcgcccagg cattacatct gggtcgttat
     2701 caccctttag atatctaccg ccgtggcgat tcacatcggg tatggctgtc ctggtacgaa
     2761 cagtattttg tatggggaat gaccgcaggc ataattcgtg agctggcgct gcaaattggt
     2821 gtgaaaccct gactatactt atctttacat ctacaaaaca ctacttgaga caatcatcgc
     2881 aatattagtt aaatcgcggt ttttgattag tttaattcat gtgaatagtt aagccagtcg
     2941 ccgcgttccc tcttacacta tgcgctgtta ttagttcgtt actggaagtc cagtcacctt
     3001 gtcaggagta ttatcgtgat tagtctattc gacatgttta aggtggggat tggtccctca
     3061 tcttcccata ccgtagggcc tatgaaggcg ggtaaacagt tcgtcgatga tctggtcgaa
     3121 aaaggcttac tggatagcgt tactcgcgtt gccgtggacg tttatggttc actgtcgctg
     3181 acgggtaaag gccaccacac cgatatcgcc attattatgg gtcttgcagg taacgaacct
     3241 gccaccgtgg atatcgacag tattcccggt tttattcgcg acgtagaaga gcgcgaacgt
     3301 ctgctgctgg cacagggacg gcatgaagtg gatttcccgc gcgacaacgg gatgcgtttt
     3361 cataacggca acctgccgct gcatgaaaac ggtatgcaaa tccacgccta taacggcgat
     3421 gaagtcgtct acagcaaaac ttattattcc atcggcggcg gttttatcgt cgatgaagaa
     3481 cactttggtc aggatgctgc caacgaagtg agcgtgccgt atccgttcaa atctgccacc
     3541 gaactgctcg cgtactgtaa tgaaaccggc tattcgctgt ctggtctcgc tatgcagaac
     3601 gaactggcgc tgcacagcaa gaaagagatc gacgagtatt tcgcgcatgt ctggcaaacc
     3661 atgcaggcat gtatcgatcg cgggatgaac accgaaggtg tactgccagg cccgctgcgc
     3721 gtgccacgtc gtgcgtctgc cctgcgccgg atgttagttt ccagcgataa actgtctaac
     3781 gatccgatga atgtcattga ctgggtaaac atgtttgcgc tggcagttaa cgaagaaaac
     3841 gccgccggtg gtcgtgtggt aactgcgcca accaacggtg cctgcggtat cgttccggca
     3901 gtgctggctt actatgacca ctttattgaa tcggtcagcc cggacatcta tacccgttac
     3961 tttatggcag cgggcgcgat tggtgcattg tataaaatga acgcctctat ttccggtgcg
     4021 gaagttggtt gccagggcga agtgggtgtt gcctgttcaa tggctgctgc gggtcttgca
     4081 gaactgctgg gcggtagccc ggaacaggtt tgcgtggcgg cggaaattgg catggaacac
     4141 aaccttggtt taacctgcga cccggttgca gggcaggttc aggtgccgtg cattgagcgt
     4201 aatgccattg cctctgtgaa ggcgattaac gccgcacgga tggctctgcg ccgcaccagt
     4261 gcaccgcgcg tctcgctgga taaggtcatc gaaacgatgt acgaaaccgg taaggacatg
     4321 aacgccaaat accgcgaaac ctcacgcggt ggtctggcaa tcaaagtcca gtgtgactaa
     4381 tacttcttac tcgcccatct gcaacggatg ggcgaattta tacccgcttt ctcgtctgct
     4441 gtaatattcc ccactacact tccactgttg cgtcaggcgt ttgtcgccat acgcttacag
     4501 ggtggcccgc atgcaaaaag cacaacggat cattaaaacc tatcgccgta atcgaatgat
     4561 tgtttgtacg atttgcgcac tcgttacgct cgcttcgacc ctgagcgtgc gatttatttc
     4621 acagcgtaac ttaaatcaac aacgggtagt acaattcgcc aatcacgctg tagaggaatt
     4681 agataaagta ctgcttcccc tacaggcagg tagcgaagtc ttgcttccgc tgattggtct
     4741 gccctgctct gtcgcccatt tgccattacg taaacaggcg gcaaaactcc aaactgtgcg
     4801 atccattggc ctggtgcaag acggcacact ctattgctcc agcatttttg gttatcgcaa
     4861 tgtgcccgtc gtggacattc tggctgaact tcctgcaccg caaccacttt tacgcctgac
     4921 gatcgaccgt gccctgatta aaggcagtcc ggttttgatt caatggacgc ctgcagcggg
     4981 cagtagcaat gctggggtca tggagatgat taacatcgac ttactgacgg caatgctcct
     5041 tgagccacaa ctgccgcaaa tcagtagcgc cagcctgacg gtggacaaac ggcatttgct
     5101 ctatggtaat gggctggtag attcccttcc gcaacctgaa gacaatgaaa actatcaggt
     5161 ttcttcgcaa cgctttcctt ttaccattaa cgttaatggt ccgggggcta cggcgctggc
     5221 atggcactat cttccaacac aattaccgct ggcggtgctg ctaagtttac tggtgggcta
     5281 catcgcctgg ctggcgaccg cttaccggat gagcttttcc cgcgaaatca atctgggcct
     5341 ggcgcaacat gagttcgaat tgttctgtca gcctttgctt aatgcgcgca gccagcaatg
     5401 tattggtgta gagattttgc tgcgctggaa caatccgcgt cagggctgga tttcaccgga
     5461 tgtgtttatt cctatcgcgg aagaacatca tttaattgtg ccactgaccc gctatgtgat
     5521 ggcagaaacc attcgtcagc gccatgtttt cccgatgagt agtcagtttc atgttggcat
     5581 taacgtcgca cccagccatt ttcgccgtgg tgtgctgata aaagatctca atcagtactg
     5641 gtttagcgct aacccgattc agcaactgat cctcgaaatc accgaacgcg atgccttact
     5701 ggatgttgat tatcggattg cccgcgagct acatcgtaaa aacgtcaaac tggcgattga
     5761 tgacttcggc accggcaaca gttcattttc ctggcttgaa acattacgtc ctgacgtgct
     5821 gaaaattgat aagtcattta ccgcagctat aggttctgac gcggttaact cgacggtgac
     5881 cgatatcatc atcgcgctgg ggcaaagact gaatattgaa ctggtggcgg agggcgtgga
     5941 aacgcaagaa caggcgaagt atttgcgccg tcatggcgtg catattttgc aagggtattt
     6001 gtacgcacag ccgatgccgc tacgtgattt tcccaaatgg ctggcgggca gccaaccgcc
     6061 gcccgcccgg cataatggac atatcacgcc cattatgccg ttacgttaac gcttactcat
     6121 cttcatcgtg cgccggttgc tctttaacaa tgcgaaccag atcaacacga taatcattgg
     6181 cttcaatgat ggtgatatgc agtggcccta catcaatcac atcgcccaca cggggaatgt
     6241 gaccatttgc cgagatcacc aggcccgcga ccgtcgcgat atcatcgtca tcggcaaggt
     6301 gctcaacatc aagcgcctgc tgcaaggcat gcaaatctgt accgcctttt accagccagc
     6361 cgtcaccatc ggtaatgatt tccggcgttt cgtcagcgtc cgggaattca cccgcaatgg
     6421 cttccagcac atccagcggc gtgaccaagc cttgtaccac accaaactcg ttggtcacga
     6481 taacaaagct cccgcgagcg cgacgcagca cgcccagcag gttgatcgga tcgagggttt
     6541 ccgggacgat aatcgccgga gacgccgaag caatcgccgc cacatcaacg ccctcttcca
     6601 gcgccaccag cagttcttta gcacgtacga ttccgatgat ttcatccagt tcaccgcgac
     6661 ataccgggaa caggctgtgc ggtgaggaga gcagctgctc gcggatttca tcgaccccga
     6721 gattagcgtc aacccagctt atttcaccgc gcggcgtcat gatcccgcgc agagaacgcg
     6781 acgccagcgt cagtacgccg ttaatcatgt aacgttcttc ttcggcaaat gcaccttccg
     6841 ggatcggcat cggcatcggg ttatcggcat cgtgctgaac attggcctga cgtttcccgc
     6901 ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc gactggtggc
     6961 gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag aagccaatcg
     7021 cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc agactcagac
     7081 caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg ttaacgaatc
     7141 gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc gccgccatca
     7201 tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac gagaagacgg
     7261 cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct ttaccgtggc
     7321 cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct ttgaacagca
     7381 agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa tccatgacgg
     7441 taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc cccagacgca
     7501 taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt ggcggcagtt
     7561 tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt tcgagaacaa
     7621 caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg acaagctcct
     7681 gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa aatgcaaacg
     7741 aaaggtgcgg catagagagc cagaaaggta ggcgttaaaa ggcctgatgc tgaaatgacg
     7801 tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta aacgggagtt
     7861 aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca aacgaatgtg
     7921 acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac ggatcttcat
     7981 cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg tttttggttg
     8041 tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaatacatc tggcacgttg
     8101 aggtgttaac gataataaag gaggtagcaa gtgaccattg ctattgttat aggcacacat
     8161 ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc tgttaggcga gcaggaaaac
     8221 gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga aaagtacaac
     8281 gctcagttgg caaaactcga caccactaaa ggcgtgctgt ttctcgttga tacatgggga
     8341 ggcagcccgt tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta tgaagtcatt
     8401 gcaggcgtta acattccaat gctcgtggaa acgttaatgg cccgtgatga tgacccaagc
     8461 tttgatgaac tggtggcact ggcagtagaa acaggccgtg aaggcgtgaa agctctgaaa
     8521 gccaaaccgg ttgaaaaagc cgcgccagca cccgctgccg cagcaccaaa agcggctcca
     8581 actccggcaa aaccaatggg gccaaacgac tacatggtta ttggccttgc gcgtatcgac
     8641 gaccgtctga ttcacggtca ggtcgccacc cgctggacca aagaaaccaa tgtctcccgt
     8701 attattgttg ttagtgatga agtggctgcg gataccgttc gtaagacact gctcacccag
     8761 gttgcacctc cgggcgtaac agcacacgta gttgatgttg ccaaaatgat tcgcgtctac
     8821 aacaacccga aatatgctgg cgaacgcgta atgctgttat ttaccaaccc aacagatgta
     8881 gagcgtctcg ttgaaggcgg cgtgaaaatc acctctgtta acgtcggtgg tatggcattc
     8941 cgtcagggta aaacccaggt gaataacgcg gtttcggttg atgaaaaaga tatcgaggcg
     9001 ttcaagaaac tgaatgcgcg cggtattgag ctggaagtcc gtaaggtttc caccgatccg
     9061 aaactgaaaa tgatggatct gatcagcaaa atcgataagt aacgtattgt gttgattatc
     9121 actcagtttt cacacttaag tcttacgtaa acaggagaag tacaatggag attaccactc
     9181 ttcaaattgt gctggtattt atcgtagcct gtatcgcagg tatgggatca atcctcgatg
     9241 aatttcagtt tcaccgtccg ctaatcgcgt gtaccctggt gggtatcgtt cttggggata
     9301 tgaaaaccgg tattattatc ggtggtacgc tggaaatgat cgcgctgggc tggatgaaca
     9361 tcggtgctgc agttgcgcct gacgccgctc tggcttctat catttctacc attctggtta
     9421 tcgcaggtca tcagagcatt ggtgcaggta tcgcactggc aatccctctg gccgctgcgg
     9481 gccaggtact gaccatcatc gttcgtacta ttaccgttgc tttccagcac gctgcggata
     9541 aggctgctga taacggcaac ctgacagcga tttcctggat ccacgtttct tctctgttcc
     9601 tgcaagcaat gcgtgtggct attccggccg tcatcgttgc gctgtctgtt ggtaccagcg
     9661 aagtacagaa catgctgaat gcgattccgg aagtggtgac caatggtctg aatatcgccg
     9721 gtggcatgat cgtggtggtt ggttatgcga tggttatcaa catgatgcgt gctggctacc
     9781 tgatgccgtt cttctacctc ggcttcgtaa ccgcagcatt caccaacttt aacctggttg
     9841 ctctgggtgt gattggtact gttatggcag tgctctacat ccaacttagc ccgaaataca
     9901 accgcgtagc cggtgcgcct gctcaggcag ctggtaacaa cgatctcgat aacgaactgg
     9961 actaacaggt gagcgaaatg gttgatacaa ctcaaactac caccgagaaa aaactcactc
    10021 aaagtgatat tcgtggcgtc ttcctgcgtt ctaacctctt ccagggttca tggaacttcg
    10081 aacgtatgca ggcactgggt ttctgcttct ctatggtacc ggcaattcgt cgcctctacc
    10141 ctgagaacaa cgaagctcgt aaacaagcta ttcgccgtca cctggagttc tttaacaccc
    10201 agccgttcgt ggctgcgccg attctcggcg taaccctggc gctggaagaa cagcgtgcta
    10261 atggcgcaga gatcgacgac ggtgctatca acggtatcaa agtcggtttg atggggccgc
    10321 tggctggtgt aggcgacccg atcttctggg gaaccgtacg tccggtattt gcagcactgg
    10381 gtgccggtat cgcgatgagc ggcagcctgt taggtccgct gctgttcttc atcctgttta
    10441 acctggtgcg tctggcaacc cgttactacg gcgtagcgta tggttactcc aaaggtatcg
    10501 atatcgttaa agatatgggt ggtggcttcc tgcaaaaact gacggaaggg gcgtctatcc
    10561 tcggcctgtt tgtcatgggg gcattggtta acaagtggac acatgtcaac atcccgctgg
    10621 ttgtctctcg cattactgac cagacgggca aagaacacgt tactactgtc cagactattc
    10681 tggaccagtt aatgccaggc ctggtaccac tgctgctgac ctttgcttgt atgtggctac
    10741 tgcgcaaaaa agttaacccg ctgtggatca tcgttggctt cttcgtcatc ggtatcgctg
    10801 gttacgcttg cggcctgctg ggactgtaag actgttgtac actaccgggg ccttttggcc
    10861 ccgttttttt atctggagga ttaatgacaa tcacggacct ggtactgatt cttttcatcg
    10921 ccgcactcct ggccttcgcg atctacgatc agttcatcat gccccgccgt aacggcccca
    10981 ccctgctggc aattcctttg ctccggcgtg gtcgcatcga tagcgttatc ttcgtcggat
    11041 tgattgtcat tcttatctat aacaacgtca cgaatcatgg tgcgttaata acgacatggt
    11101 tattaagcgc actggctctg atgggttttt atatattctg gatccgcgtt ccgaagatca
    11161 tctttaaaca aaaaggtttt ttcttcgcca atgtctggat tgaatatagc cgaatcaaag
    11221 cgatgaactt gtcggaagat ggcgtgttgg tgatgcaatt agaacagcgt cggctgttaa
    11281 tccgcgttcg aaatatcgac gatctggaaa aaatttataa gcttctcgtt tcaactcaat
    11341 aagttatgaa tttagccaaa gctatgttta gtgtattttt a
//
LOCUS       DAPHDX010000136        11191 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPHDX010000136 DAPHDX010000000
VERSION     DAPHDX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.138"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            40..240
                     /locus_tag="RH058_003685"
                     /pseudo
     CDS             40..240
                     /locus_tag="RH058_003685"
                     /inference="COORDINATES: protein motif:HMM:NF025054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            678..824
                     /locus_tag="RH058_003686"
     CDS             678..824
                     /locus_tag="RH058_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139217.1"
                     /translation="MVDLFGCTGAIAGQVPAVGSEVLSRVIASGLKVLTRVLVAGDEV
                     LPRE"
     gene            complement(959..2559)
                     /locus_tag="RH058_003687"
                     /pseudo
     CDS             complement(959..2559)
                     /locus_tag="RH058_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2566..2958)
                     /gene="tnpB"
                     /locus_tag="RH058_003688"
                     /pseudo
     CDS             complement(2566..2958)
                     /gene="tnpB"
                     /locus_tag="RH058_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2945..3274)
                     /locus_tag="RH058_003689"
     CDS             complement(2945..3274)
                     /locus_tag="RH058_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3139218.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLLLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            complement(3274..3820)
                     /locus_tag="RH058_003690"
                     /pseudo
     CDS             complement(3274..3820)
                     /locus_tag="RH058_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522305.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3823..4100)
                     /locus_tag="RH058_003691"
                     /pseudo
     CDS             complement(3823..4100)
                     /locus_tag="RH058_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046881606.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(4461..5369)
                     /locus_tag="RH058_003692"
     CDS             complement(4461..5369)
                     /locus_tag="RH058_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3139219.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            5496..6854
                     /locus_tag="RH058_003693"
     CDS             5496..6854
                     /locus_tag="RH058_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HDV3139220.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYATGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6866..7894
                     /gene="gtdA"
                     /locus_tag="RH058_003694"
     CDS             6866..7894
                     /gene="gtdA"
                     /locus_tag="RH058_003694"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HDV3139221.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEDQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRMGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFSSRVARTTD
                     STIYHVVEGSGQVTIGNEIFTFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            7910..8611
                     /locus_tag="RH058_003695"
     CDS             7910..8611
                     /locus_tag="RH058_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV3139222.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDKQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVIPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            8620..9264
                     /gene="maiA"
                     /locus_tag="RH058_003696"
     CDS             8620..9264
                     /gene="maiA"
                     /locus_tag="RH058_003696"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HDV3139223.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            9279..10472
                     /locus_tag="RH058_003697"
     CDS             9279..10472
                     /locus_tag="RH058_003697"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HDV3139224.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGTQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTNRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRIYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            10822..10962
                     /locus_tag="RH058_003698"
     CDS             10822..10962
                     /locus_tag="RH058_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139225.1"
                     /translation="MPPHKIRQEWLALDETAEVILQHSITLQSADLIRHSFIWRNRSI
                     CL"
BASE COUNT         2562 a         2914 c         2973 g         2742 t
ORIGIN      
        1 tggcatcgca ttaacaagaa atgatggtga aaatataaac ttgctgatat aataattaat
       61 gaaggttaca gggggtttgg agtagggagc gttcttttaa caaaagcgat agaatcagca
      121 tcatcactcg gggtaaggaa aatttacggt gtcatgtgcg gagatactaa aagattaatc
      181 aaattctaca aatcctttgg gtttgaaatt cacggtaata gtatagagct ccatctataa
      241 atagataaca caagaactct atccaaacat aataatcccc tgaggcaggc agcagtgatc
      301 actgagagca ttgcatggaa gcgccagtga tacccctacg cagtatcctg acgccctgag
      361 cgactatccc gtcctctgtt ctctcatccc tgacgctgaa acccggcatc actactgcct
      421 cagtgcttgc tggcctgacc ttattggccc gtaatcaccg taagccggga actggcgttg
      481 tacgagtggc acttgccatc aatgcgagtt caccgcaacg ttacgaattt ggttaattat
      541 tcatcctgca attgctaatt gttgagaaaa ccagctctgt atcgctcaag tgtaaggtaa
      601 gcttccgggc atctcatctt gacgtgtttt tcgggcgctc gtcatcggcg gcgcatagga
      661 acgatcgtgc tctggcgatg gttgatttat ttggttgcac aggggcgatc gccgggcagg
      721 taccggcggt gggctctgaa gtactgagtc gggtaattgc gtcaggtctc aaagtactga
      781 cacgggtact ggtggcaggt gatgaagtac tgccccggga atgactccgg gctgattcag
      841 gttcttattc gctgtcagcc cgcgtggttg ctgccctgac gcatttcctg tcgacaggcg
      901 ttcaactccc gactgcaggt tatagcccga tgcacaaaca cctgacggca ggtatcactc
      961 acccggagaa ggtaaagccc tcaagaggta gcaactctgc cagtcggtcc tccggccact
     1021 ccggcagacg cgtcaggacg tctgtcagcc aggcatgtgg ctccagaccg ttgcgtttcg
     1081 cggtttccag caagctcatt atttgcgcgg cgcgttctcc cgccatctgc gaaccggcga
     1141 acagccacga ttttctgccc agagccacgt ttttgatggc ccgttcacac acattattat
     1201 ccagcggcac cgcaccatct tccaggaagc agctcagttc cacgcgatga gacagcgcat
     1261 agacaatggc tttgtgtaat gcccttcccg gaaaacattg cggttcctgc tcttcaagcc
     1321 atgaccacag ttcttccagt atcggacggg cataacgctg tcgccactgg cggatttttt
     1381 ccacggggcg gctactgatc ttcttctcaa gacggtacaa ccccgcgatt ttcttcatgg
     1441 ctatggcagc ccgtggatct ttactgattt tatacaggtc ggcgaagccc ctgcgggcat
     1501 ggcccagcat ccggccagcg tgatctccgg cactttgttt gccagagtcc ggtaagcttt
     1561 atgtccgtcg acaaccagcg tcccgcccca gccctgaagc cagttttcag gatactcatg
     1621 gctacgtccg gtccggcagt tgaagcccac aactgacggt cccgtccttt ctccactgac
     1681 gtatgcccac agataaccgg agcaggattt accgcctttc ttcgtgttca ggagcttcag
     1741 ggtagtctca tctgcatgca ccaccggacg gtttatcaac tcgcgatgga gtaacgccgc
     1801 caggggagat aatgcggcac ccgccgcgcc aaccatgtca gccatcgaac tgacgggaag
     1861 cccgacatct gaacgggcaa agacctgttg ctggcgatac agaggcaggt ggtcaccgta
     1921 tttattgatg atcacctgtg cgatgactga tggctcaacg gcacttttcg ggaggatatg
     1981 tgccggcatt tcaccgctga acaccttctg gcaacaggga caactgtatt gcggacggac
     2041 ataacagttc accacgaagt gagcgggaat atactccagc ttttcactca ccgcatcacg
     2101 gatatagtgc agcggctcgt cacattcagg gcaatggtca gtgtccggct ggataatttt
     2161 ttccacccgt ggaagatgag ccggtaagga tttgcgtatc gggcgtgaac gggacgcttt
     2221 ttcgtcttct tcaggggatt gcgggagcag tttatccaga tgtgcggtaa gcgcggcgat
     2281 atcgacatca acatcctctt cgaacaggga gcgttgcata ccagccagcg tttcacattt
     2341 tttaccgaag cgctgttgac gaaccagttt cagcatttct tccagaagct ggatgcgccg
     2401 gcttttctcc agtaattccc gctctttttc ggcattctcc gacatgactt tttgtaccat
     2461 ggcaagtgcc atggcacgca gtttttcgat gtcattcgtg gtgttgagag cggagatgtc
     2521 catacagggg agcataccac tccggatacg gatattaatt atttttcaat tagttagcag
     2581 tgtattttgc gtgttttcag ggcgcagttc aggttcattc tgccagaccc atttcgtcag
     2641 gtcatgtccc ttaacctgct gccagtcaac accggcaatc agccagttaa actcgtcggg
     2701 agtgaggtgc taggcagcgt catttgcaca tggccagcgg aagctgcctt tgtgcagacg
     2761 gcgggtacac aaccacaccc cgtgtttatc ccaccggaga actttgatac gcgaacgggc
     2821 tttgttaaca aagacgaagg cggctccccc atgccatatc gaccggagtt catcctgtat
     2881 atactgcgtc agtgcgtcga tgccccggcg catgtcaaca ggtttaattg cgatgaagac
     2941 attatcagga ctgagcatat ttgagcgccc tgaagacatc ggtaagttgt gacggctgac
     3001 agcacatccg tactccaccg ggaaggagga gcgtgactgg ctcattcgtg acgggctgtg
     3061 gtacatcagc atgcagtggt ggcgcaatat gaacgggaag aacggtcggt tcatttgccc
     3121 ggcgttcagc ttttgcgaca tcctgcggcc attcgcgaag tgatgtgaag ggaatatcgt
     3181 tgagttcaca gtattgctga cgtgtaagac cactggcgcg ccaggcagca acgtgctgcc
     3241 ttttttgttc aatggtccat cggggttttg acatcaatta tctccggtaa gttgatgaat
     3301 gaatgtcggt aatgctaact gactgaaaat cgctgtgaag atgacttccc cgtaacgaca
     3361 cagaatatct tccgcaatac gctgactacc agaccagaac agaccgtcca gtgcggtcac
     3421 cagtaatccc tcctctagcg catacttaag tcattcacgg agcgctttca ctttccgggc
     3481 aatctgctgc caaggcgatg atgtatttca ttcccaacca tccggcgaat acggtgagac
     3541 agatgatacc ggaatgtatc cgcacgccgg aaagactggc cttcaggcta tacacgcaac
     3601 caaagcgttt atcgttaaac accacatttt tctggctgat atcccattct tcccaaagca
     3661 cagcaatcag ctgtgacgtg cgggttaaaa gcagacgaaa aggcagttca aaactggtgg
     3721 tataatccac attcagcagg gcattacgca gccgctctcc tgatccgacc tctgcctgcc
     3781 gacactcctc ctgaacatcc tgccactgct ggcgaaggcg tacaacaaca cgaacattct
     3841 cagcgtattt ttttgagtaa ccaactgaaa aatggtggga agatcatttt tcagttgaga
     3901 attccccaac ggtcaatcca cccgctttat cttgttttta actaatgctg tctggttaaa
     3961 tgacgcccga tgctgctaag cgtgagggaa gcgccattta ttacagcatt tgttgcgtca
     4021 atcagtgatt tttgatggta gaaatgcaaa gtggccagta tgtttttaag gaatttttgg
     4081 cataatgaac gtgcaggcat agtggtgata tccttgaaat tgctagcaca atcaattaga
     4141 tcacatctca ctatgcctgt cttgttttct gggaattcct aatgggttgt ccttccaatt
     4201 ggttctttcg aggcctgctc agattcactc acattataac ctgtatactc actgaaacac
     4261 ctacagcttc ttcatcaaat acatcagccg ctttgttacc cacacgactg ctttgactgc
     4321 taccgcctgg aacgacagtc acctggtggg attttcaccc catgaaagat cacgccttat
     4381 cacagtgcac gattccccga aaaattacgc atcgttgaat tacagggaag taacatcgtg
     4441 cgaatgccca gttcagcaca ctaattctgc tgtaaaaagt gcagaaactg acttaaagca
     4501 taagaaggcg tttcgttatt gcgccagaac acaccgacgc tgcctttctg ttctatcttt
     4561 ggcaggttga gtatcaccag ttgtccctgg ctggcataac gttgcgccag gcgtaatgac
     4621 aaaatagaga tcatatcgct gctttgtaac aggttggtac tgacgttcat cgacgccgac
     4681 tcaatcgtgt tttctggcag cattacgccg tggtctacca gcgcattatc aatactaatg
     4741 cgaatggggg tacccgtcgg ccagacgatc cagcgccagt tggcaagatc attccagctg
     4801 agggtggcgt atttcgccag tgggtgctca gggcgggcga caaagcatac tggctcggtg
     4861 tacagcacct gataattcag tggcagttgc aaggcgcgac cgccaattcg cccaacgacc
     4921 acgtcgacag aacctgccaa cagatcttgc agcaatgggg tcatcacctt ctcttcgata
     4981 ttcaggtgta gagttggcat cttcgtaagc aaatgcaaaa tcgcctggga tacgcagtca
     5041 gtggcgacgg gagaacagcc aattttcaga ctgccgacca gtccaccttg tttaaaacgg
     5101 gccagttcat gttgcgatcg ctccatgtca ttgattaacc gttgggcatg ttgcaataac
     5161 aatttgccgc cttcagtcgg acgtaatcct ttgctgtgcc gctcaaaaag tgtgattcca
     5221 atctcatctt cgagttgtga caaccatttc gaaagcgcag gttgggtgat attcatcatt
     5281 cgtgcgacat gggtcagatt gccctgttca cccagcgcca ccagcatttc aaggtgatgt
     5341 agttttaatt tttgcgccca gtttgccatt taccataacc tccaggttat ctctctgccc
     5401 gaaatgccat tgtacatttc atctctttaa tcacaatatt gcaacatata caaaacaaaa
     5461 gaaacatcta ccttacctgc actgaggcat ttactatgac tcaacgacgt gatctacaag
     5521 cacttatcga tgctgctccg gtcggcaaga tgcagtggcg cgttatcatc tgctgttttc
     5581 tggtggtaat gctcgatggt tttgacaccg cggctatcgg atttatcgcc cctgatattc
     5641 gcactcactg gcaactcacc gcaggcgatc tcgccccgct ttttggcgcg ggtctgctgg
     5701 ggttaaccgc aggcgcactg ctttgtggcc cgttgtccga tcgctttggt cgcaagcggg
     5761 tgattgagct gtgcgtcttt ttgtttggcg cgttaagcct cgcctcagct ttctctccgg
     5821 atctgcaaac tctggtcttc ctgcgctttc tcaccggcct gggtctgggc ggcgcgatgc
     5881 cgaacaccat caccatgacc tcggagtatc tgcctgcccg ccgccgcggc gcgctggtga
     5941 cgctgatgtt ttgcggcttt acgctgggtt cggcgtttgg cgggattgtc agtgcgcaac
     6001 tggtgccggt gattggctgg cacggcattc tggtgttggg gggcgtgtta ccgctgatgc
     6061 tgtttgtcgc actgctggta gttctgccgg aatcgccacg ctggcaggtg cgccgccagc
     6121 taccgcaggc ggtgattgcg aaaaccgtca gcgccatcac ccgcgagcgt tatgtcgata
     6181 cccattttta tctcattgaa tcagcctccg ttacaaaagg cagcattcgt caactgttta
     6241 tgggacgtca gttacccatc acgttgatgc tgtgggtagt gttctttatg agcctgctga
     6301 ttatttacct gctctcaagc tggatgccga cgctactgaa ccatcgcggc atcgacctgc
     6361 aacacgcgtc ctgggtcacc gccgcgttcc agattggtgg tacgttaggc gcgctggcgc
     6421 tgggtgtgct gatggataag ttcaatcctt tccgcgtact ggcgctgagc tatgccacag
     6481 gcgctatatg catcgtgatg attggcttaa gccaggatgg attatggctg atggcgctgg
     6541 cgattttcgg cactggcatt ggcattagcg gatcgcaggt ggggctgaat gcgctgaccg
     6601 caacacttta tccaacacaa agtcgcgcta cgggcgtgag ctggtcaaat gctatcggcc
     6661 gctgcggcgc gattgttggt tcgctgtctg gcggcgtgat gatggcgatg aatttctctt
     6721 tcgacaccct gtttttcatt atcgctgtgc ctgcggcgat cagtgcggtg atgttaaccc
     6781 tgctgattac cgtggttcgc cagtcgacct ctgtccctga ctcactgcct cgcgcaggcg
     6841 ttgtgaacga ataaggagca tcccaatgac tgataacaat caaaatagcc gcgagcaatt
     6901 ttatcagcat atttccgggc aaaacctgac cccgctgtgg gagtcactgc accatctggt
     6961 gccgaaaacg cccaacgcta actgtgcgcc agcgtactgg aattatcagg agatccgccc
     7021 gctgctgctg gagagcggca gcctgattgg cgcgaaagag gcggtgcgcc gcgtgctggt
     7081 actggaaaac ccggcgctgc gagggcagtc ttccatcacc gcgactttat atgccggttt
     7141 gcaactgatc atgccgggcg aagtggcacc gagtcatcgt cataaccagt cggcgctgcg
     7201 ttttattgtt gaaggcaaag gggcatttac cgccgttgac ggcgaacgca cgccaatgaa
     7261 tgagggcgat tttatcctga ccccgcagtg gcgctggcac gatcacggca accctggcga
     7321 cgaaccggtt atctggctcg atgggttgga tctgccatta gtgaacactc tgggctgcgg
     7381 ttttgccgaa gattacccgg aagatcaaca accggtaacg cgtaaagagg gcgattacct
     7441 gccgcgttac gctgccaata tgttgccgct gcgccatcag acggggaact cctcgccgat
     7501 ctttaactat cgttatgacc gcagccgcga agtgctgcac gatttaaccc gcatgggcga
     7561 tgccgacgaa tgggatggct acaaaatgcg ctacgtcaac ccggtcaccg gcggctatcc
     7621 gatgccgtcg atgggcgctt tcctgcaact gctaccgaaa gggttctcct cccgcgtggc
     7681 gcgcaccact gacagcacta tctaccatgt agtggaaggt agcggacaag tcaccatcgg
     7741 caatgaaatc ttcaccttta gtgcaaaaga tatctttgtg gtgccgacct ggcacggcgt
     7801 gtcattccag actacgcaag ataccgtttt attcagtttt tcggacagac cggtacaaga
     7861 agccctgggg cttttccgcg aagcgcgtta ttaatttggg agagagaaaa tgactcaata
     7921 tgtattcgaa ccacaagcgc ccgttaccgt accggttgtt ggcagtgata aacaattccc
     7981 cgtgcgtcgc gtttactgcg tcgggcgtaa ctatgccgcc cacgcccgcg aaatgggctt
     8041 tgatccggat cgtgaaccac ccttcttctt ctgcaaacca gcagatgccg ttattccggt
     8101 cgccgctggc gaaacgctgg cgctgccata tccggcgcag accgataact atcattacga
     8161 aattgaactg gtagtggcga ttggtaaaaa aggcagcgat atcccgctgg aacacgccca
     8221 tgaatatgtc tggggttatg ccacgggtct tgatatgact cggcgtgacc gtcaaatgga
     8281 aatgcgccag atgggccgcc cgtgggaaat cggtaaagcg tttgatctct cagcgcccat
     8341 cgcgccgctg cacaaagccc gcgatatcgg cggtattgat aatgcgccaa tctggttaca
     8401 ggttaacggc gaagatcatc agcgcagcga tattcgccat ctcatctggt cggtgaatga
     8461 aaccatcagc tatctgtcag gcttctttga gctgcaaccg ggcgatctga tcttcaccgg
     8521 tacgccggaa ggggttggcc cggtagtgaa aggtgatgtg atcacaggca acgtcgaggg
     8581 cttaacgcct atcgcggtga agattgtctg aggtgcatga tgaagctgta cagttttttt
     8641 aatagttcag cgtcgtaccg ggtacgcatt gcgctggcgc tgaaaggcat taattaccag
     8701 accgaagggg tgaatatccg tattggtcag caaaatgaac tggcctatcg gcggatgaat
     8761 ccggtcgggc tggtgccgac gctgctgact gacgagggcg aatcactcgg acaatcgctg
     8821 gcgattattg actggctgga gaggcactac ccgcaagttc cgctggtgcc gcaggaagaa
     8881 ccggcccgca acaaggtgct ggaaattgtc tatgccatcg cttgtgatat tcatccattg
     8941 aataacttgc gcgtgctgcg ctacctgacc gaagaactga acgtcagtga agaagaaaaa
     9001 aaacgttggt atgcgcactg gatccagcaa gggttaagcg cggtggaaca actgttgcgc
     9061 cagaaccagt cggggcaatt ttgtgtgggc gagacgccga cgctggcaga ttgctgcctt
     9121 gtcccgcagt gggcaaacgc gctacgcatg aactgcgatt tgagcgggta tccacgctgc
     9181 aaagcggtgt acgacgcctg cacacagttg ccagcgttca ttgccgcagc gccagaaaat
     9241 caacaagata aaatttcagc ctgaccagga gaactataat gacgaaagtg acacgcgcag
     9301 taattgtggg aggcgggatc ggcggtgcgg caactgcgct gtcactagcc cgcctgggga
     9361 tcaaagtggt gctgctggaa aaggcgcatg agatcggtga gattggcgcg ggcatccagt
     9421 tggggccaaa tgcgttttcg gcgctggaca gcctcggcgt cggcgaagtt gcccgccagc
     9481 gcgcggtgtt taccgatcac attaccatga tggatgcggt gaacgccgaa gaagtggtgc
     9541 gcattgaaac cgggcaggcc tttcgtgacc atttcggcgg tccttacgcg gtgatccacc
     9601 gggttgatat tcacgcctcg gtgtgggaag cggtactgac gcatccggac gtggaatatc
     9661 acacctccac caacgtcgtg gatattcgcg agacggaaga cgatgtcacc gtgtttgacg
     9721 atcgcggcaa tagctggacc ggcgatattc tgattggctg tgacggcgtg aagtcagtgg
     9781 tgcgccagag cttattgggc gatgcgccgc gtgtcaccgg acatgttgtc taccgtgcgg
     9841 ttatcgattg tgccgatatg ccggaagatt tgcgcattaa cgcaccggta ctgtgggcgg
     9901 ggccgcactg tcacctggtg cattaccctt tgcgcggcgg cacgcagtat aacctggtgg
     9961 tgacattcca cagccgccag caggaagagt ggggcgtgaa ggatggcagt aaagaggaag
    10021 tgctctctta ctttgagggg attcacccgc gtccgcgcca gatgctggat aaaccgactt
    10081 catggcgacg ctggtcaact gccgaccgtg aaccagtcgc gaaatggggc actaaccgca
    10141 ttacgctggt gggcgatgca gcccatccag tggcgcaata tatggcgcaa ggtgcctgta
    10201 tggcgctgga agacgcggta acgctgggta aggcgctggc gcagtgtgag ggtgatgcgg
    10261 cgcaagcctt tgcgctgtat gagtcggtac gtattccgcg caccgcgcgt attgtctggt
    10321 cgacccgtga gatgggacgg atttatcacg ccgcgggagt agaacgccag gttcgtaacc
    10381 tgctgtggaa agggaaatcg caggcagagt tttatcgcgg catggagtgg ctgtacggct
    10441 ggaaagaaga taactgtctg caaccacgct gagtacttct tcacctaacc aggcgcacgg
    10501 gtgatctcct tgcccttcgg agaggagcta ttaagtcctg ttgatttcaa cagggctctc
    10561 tcctctccct ttagcctcct ttaaaataag gtatcatcag aaatccttct ttcaacaaat
    10621 agcacgccac ttcaagcaaa tcgtctccag agctattcag tgttacgtcc tgtttttcca
    10681 gtgtaaatca tcaccagatt atgtctgaaa agctcgttac ctacactgat cagtgttagt
    10741 tgttttggca aaaatatctc acaccggttt tcatcatcaa caagcactat acgcaaccaa
    10801 caacacaaaa aggaacacac catgccccca cacaaaatcc gccaggagtg gctggcgctg
    10861 gacgagaccg cagaagtcat cctacagcac agcatcacgt tgcaatccgc cgaccttatc
    10921 cgccattctt ttatttggcg caaccgcagt atctgtctgt gatgttggtg ttactgccaa
    10981 gcgctttatg gttcgggata acggcatgta tggtagcaag tagctttaag cgatggttat
    11041 aagttgctca aaaaatcggt cctcactaca atacgaattt tatcaggtat ggaattcgga
    11101 ttatgaataa atctgtcata aagctgttga ataaaaaatt cattatttca gtaataacct
    11161 catctacatt tattactgga tgcacaatgg c
//
LOCUS       DAPHDX010000137        11072 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPHDX010000137 DAPHDX010000000
VERSION     DAPHDX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.139"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            71..865
                     /gene="hyxR"
                     /locus_tag="RH058_003699"
     CDS             71..865
                     /gene="hyxR"
                     /locus_tag="RH058_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDV3139226.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPASSLYNGFP
                     HIGTLLKASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATQALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVIVGKSINSDAISGKVLSGQRKHMSKKRPGVL"
     gene            862..1002
                     /locus_tag="RH058_003700"
     CDS             862..1002
                     /locus_tag="RH058_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139227.1"
                     /translation="MTQQRCYYHPLRRVRTRYSVGAKIFLLLWAAGVGGTLYSLYSVL
                     AY"
     gene            1019..1780
                     /gene="hyxA"
                     /locus_tag="RH058_003701"
     CDS             1019..1780
                     /gene="hyxA"
                     /locus_tag="RH058_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDV3139228.1"
                     /translation="MRFVLFCPSGIVPAQFEALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKTGMS
                     VERAISVANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1822..2904
                     /locus_tag="RH058_003702"
                     /pseudo
     CDS             1822..2904
                     /locus_tag="RH058_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2926..4119
                     /locus_tag="RH058_003703"
     CDS             2926..4119
                     /locus_tag="RH058_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3139229.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSL
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            4303..4968
                     /locus_tag="RH058_003704"
     CDS             4303..4968
                     /locus_tag="RH058_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139230.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(5214..5909)
                     /locus_tag="RH058_003705"
     CDS             complement(5214..5909)
                     /locus_tag="RH058_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV3139231.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5902..7329)
                     /locus_tag="RH058_003706"
     CDS             complement(5902..7329)
                     /locus_tag="RH058_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV3139232.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILSHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7340..8059)
                     /locus_tag="RH058_003707"
     CDS             complement(7340..8059)
                     /locus_tag="RH058_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV3139233.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8589..9443)
                     /gene="rclR"
                     /locus_tag="RH058_003708"
     CDS             complement(8589..9443)
                     /gene="rclR"
                     /locus_tag="RH058_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV3139234.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            9669..10994
                     /locus_tag="RH058_003709"
     CDS             9669..10994
                     /locus_tag="RH058_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV3139235.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAIGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3037 a         2589 c         2547 g         2899 t
ORIGIN      
        1 gctattaaac caaacctgac attttgaatc aaaatgtcag gttttgtagc aatggcgtgg
       61 acgagtctga atgaagaagc ctctggttat tatcagtgcc tgtcagttca ccaggctggc
      121 actggagtct ctcatcccag cggacaggta tatcgtgcgg gtgtacagca acgtcactac
      181 agaggttgaa ttcgttttaa agacctcctg tggctacctg ttggctgata tcccttcgct
      241 tccttcgggg gagttagttc ttctggcttg tcttgtccgc ttgcggagtg atgccgggca
      301 atggcaagtc tgcctgaccg gtgactccga ctggttcgat aatgctcctg cttcaagtct
      361 gtataacggc ttcccacata tcggcacctt gctgaaagcg agtcgttatc agaatcaaat
      421 tatccgctgg ttactacgtc ctttacccgg cgatatgagt gatttctggc tcctgactgc
      481 acgggagtta agcgtgctta agattctgat gcagggcatg agtttcagtg agattgcccg
      541 ttatgaacaa cgcagttcaa agactctgca tgcgattgcc acacaggcat taaggaaact
      601 tggactggga acactaagcg atttcaggct gctctataca ggatgcggtg acagcagagt
      661 caaacggaat atacggctac atgcaagata tgtcggacaa cattccgctc atcgactcac
      721 tcagtatgtg cagaacatgg tgtccggaat tctttatcca gagaaagtta tcgttggtaa
      781 atcaataaat tctgacgcca tatccggaaa agtgctttcc gggcaaagaa aacatatgtc
      841 taaaaaaaga ccaggagtgt tatgacacag cagcgttgtt actaccaccc actgcggcgc
      901 gtacggactc gctacagtgt aggggccaaa atatttctac tgctctgggc tgcaggagtg
      961 ggaggcacgc tgtacagcct gtacagcgtg ctggcgtact gacatctgtg agggaaggat
     1021 gcgtttcgtt cttttctgtc cctcaggaat tgtacctgca cagtttgaag ctctatcaac
     1081 cggttctgtc tgcaatatta cctgcatcag aacagaagaa gagttacgca ataaattgcg
     1141 gcacaggccg cagagtgtcg tgatatcggc agggcgacct gccgaatgcg ctgaaatgtg
     1201 gttccgattt tatcgggatc actcattcgt ggtggtgttg tgcgtcgcgc ccttttttct
     1261 gccaccggat gtgagtattt ctggcgttct caaaaacctg aggttactga aaacaggaat
     1321 gtcagtagag cgtgctatca gtgttgcaaa tgcctctggt gggttttcag gactgaaaca
     1381 tgcagaaaat ttgccagtta tggacagtta ctctgtcttt atgaatgagg tgaataaccg
     1441 cacaaagaca atcgtcatgt cagaacgctt cccggaaaag caaaagaagg tgttgtcgtt
     1501 gcttcttgct ggacacagct gggaatacag tgcgcagttt ctcaaaacgg ggataagaca
     1561 gatatggctt gctgagcagt cgctaaaaaa gcgttgggga ataccggaca gcatgtcatt
     1621 gcgggaagcc ctgctgctga gcagcaataa atttcacggt gatggaaatg cattggaaaa
     1681 aacgaatgcc atgacattgc gtgaaaacgg aaataccaac aggtattccg tggtggtaaa
     1741 tgcagggacg caggctttat ctctgcataa atataaataa gtgggaaatt gaaattagtt
     1801 accagaatgg agaatttctt catgaaaaac agtaaggcat tttaccgcag cgcattagcg
     1861 acagctattg ttatggctct ttctgcacca gcattcgctg ctgataacgc ggtatcaact
     1921 acaccggtta cactggataa agataagaca actctggatc aagatgttgt tattagcaat
     1981 acagcagacc aacagattac agccgtaaca attaatgcgg cagataaaga tcttaatgtt
     2041 acttttggcg gtcatgatat tactgctgaa tcaacggcag acaaaaaatt ccttgaaggt
     2101 gtaaaagtta gcggtgacaa aaatattgtg attaatgcta caggctccac catcacagct
     2161 caaggtgaag gcacctatgt ccggactgca atggtcattt cttcaactgg cgatgttgtt
     2221 gttaatggcg gtaatttcgt tgcaaaaaat gaaaaaagta gtgcgacagg aatatctctg
     2281 gaaggggcca cgggaaataa tgtaacgcta aatggtacaa ccataaatgc tcaaggtaat
     2341 aagagttcca gcaacggctc tacggcaatt tttgctcaaa agggtagcgt attgaatggt
     2401 tttaaaggtg atgcaaccga caacattacc cttgctggct caaatattat taatggccgg
     2461 attgaaacaa tagttattgc caaggagaat acgggaactc atacagtcaa tctgaatatt
     2521 aaggatggct cagtaattgg ggcggctaat aataaacaaa caatttatgc ttctgcttcg
     2581 gcacaaggag caggttcagc aacgcaaaat ttaaatttat ctgtcgccga ttcaaccatc
     2641 tactctgata tccatgccct ttctgcaagc gagaattcag ccggtaccac aacaaatgta
     2701 aacatgaacg ttgcccgctc ttactgggaa ggtaatgctt ataccttcaa tagcggcgat
     2761 aaagcgggta gtaatctgga tataaatctt tccgatagct cagtctggaa aggcaaagtt
     2821 tcaggggcag gagatgccag tgtatctctg caaaacgggt ctgtctggaa tgttacggga
     2881 tcctcaactg ttgatgctct ggcagtaaaa gacagtacgg ttaatatcac gaaggctaca
     2941 gtcaatactg gcacgtttgc ttctcagaac ggcactctga ttgttgatgc ctcttctgaa
     3001 aacactctgg atatcagcgg aaaagcgagc ggtgacttgc gtgtttacag tgcgggttca
     3061 ttggatctta tcaatgaaca aacggcattt atttctaccg gcaaagacag cactctaaaa
     3121 gccacaggca caacggaagg tggtctgtat caatatgacc tgacacaggg agctgatggt
     3181 aacttttatt tcgtaaaaaa cacgcataaa gcatccaacg ccagctccct gattcaggca
     3241 atggcagcag ctccggctaa cgttgctaat ctgcaggctg acacgctctc agcccgtcag
     3301 gatgctgtcc gtctgagcga aaatgacaag ggcggcgtat ggattcagta ctttggcggt
     3361 aaacagaaac ataccaccgc gggaaatgca tcctatgacc tggatgtaaa cggtgtaatg
     3421 ctgggtggtg atacccgctt catgactgaa gatggtagct ggctggcagg tgtagcgatg
     3481 tcttctgcga aaggtgacat gaccaccatg cagagcaaag gcgacactga aggttacagc
     3541 ttccacgctt acctgagccg ccagtataac aacggtatct tcattgatac tgctgcacag
     3601 tttggtcact acagcaacac ggcagatgtt cgcctgatga atggtggcgg taccatcaaa
     3661 gctgacttta acaccaatgg tttcggtgcg atggttaaag gcggttacac atggaaagac
     3721 ggtaatggcc tgtttattca gccatatgcc aaactgtctg cgctgacgct ggaaggtgtg
     3781 gattatcagc tcaacggcgt ggacgttcat tctgacagct ataactctgt gctgggtgag
     3841 gccggtacgc gcgtgggtta tgacttcgct gtgggcaact cgaccgttaa accttatctg
     3901 aatctggccg cactgaacga attctctgat ggcaacaaag tccgtctggg tgatgagtct
     3961 gtcaatgcca gcattgacgg tgcagcattc cgcgtgggtg caggtgtaca ggctgatatc
     4021 accaaaaaca tgggagcata tgcaagcctt gactacacca aaggtgacga cattgagaac
     4081 ccgctacagg gtgtagttgg tatcaatgtg acctggtaag aataaagcag tcggttcccc
     4141 tgtcgactgt tataccccct gcaggcagca tactgctgcc tgcaggctat aaacatccag
     4201 tattcatgat gctggttttt tgctgatatt taccccgaac attcctgata caggctatta
     4261 atacaacctt atctgcggca gattttattt tctggatata ttatgcgtga acaaatcaaa
     4321 caggatatcg atctgattga gattttattt tatctgaaga aaaagattcg tgttatcctt
     4381 tttattatgg ctatatgtat ggctatggtg ctgttgtttc tgtatatcaa taaagacaat
     4441 ataaaagtga tttacagcct aaaaataaac cagacaacgc caggtatact tgttagctgt
     4501 gatagcaata ataattttgc ctgtcagacc acaatgactg aagatgttat tcagcgaatt
     4561 actacatttt ttcacaccag cccagatgtc aagaacagag aaataaagct ggaatggtca
     4621 ggagataaga gagatttacc aactgctgaa gcggaaatat ctcgcgtgca ggcgtctatt
     4681 atcaaatggt atgcgtcaga ataccataat ggcaggcaag ttctcgatga gatacaaaca
     4741 ccttcagcaa ttaacagtga gctttataca aaaatgatat acctgaccag gaactggtca
     4801 ttgtatccga acggtgatgg ctgtgtaact ataagctcac cagaaataaa aaataaatac
     4861 cctgctgcca tttgcctggc tctgggattt tttctaagca ttgtaatttc tgtaatgttt
     4921 tgccttgtca aaaaaatggt agatgaatac caacaaaact ctgggtagta ataaacagca
     4981 ttctgataga gagtgcctgt ataaagctga agtgacaatg accgggaagg gaacccgttt
     5041 tcaggaatgg atgcctgtct ttttcactgt aaatatcctg gcattactaa tgccaggata
     5101 tttacagaag agcagcataa ccatgacgga acggggttaa gtcaggttgt ccttaacgct
     5161 attttcaacc tcgtatgcct tcttcaggtt tatgttcaga tctcatatct ctctcagcaa
     5221 tcctcaataa tcagatacac cgctttcacc ggaccatgaa ccccgacgac tttgataagc
     5281 tcaatatccg ccgttgaact agggccgcta atgatgttaa tgcaggacgg cattcgttca
     5341 ccggcctgcg ctttctgatg caatttttct gcgagttgcg ctacacgcgg caggattgtg
     5401 cttttacgca ggataaaaag agaagattcc gggagcaggc tcaatgaacg cccgcgcccg
     5461 gcggcggaaa aaagaaccac gcctcccgat tcggttaaac catattcagc atacacgaca
     5521 cccactttag cctgctcggc ctgcgagata ttctcggcac ctttcgccgg atcccaaaca
     5581 acggcattgc attcctgctg caaacgttca ctaatcccca attcctcgag cctcgtgtca
     5641 ccgctaatca cgaccgactg atctcccagc tctttacaca gacgtattgc agcttctgcc
     5701 gccttcgcct cgctggtcag ctcacagcgc gtcaacataa catcgctggc aaactgaata
     5761 aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc gctcgttagc atagttgtta
     5821 agcggcgcat cttctgcttg cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg
     5881 ttattcaaaa attcgcctcg attatccatt ctttttctcc tgcgcctgat gtttcttaaa
     5941 ccaactacgg aaactctctc cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct
     6001 aatcgcgcca aatttgagtg gtgttttgcc gccattgata aaccagcttg ccgcatgagc
     6061 gccggccatc atcccgactt tccacaatcc tggatgacta ttggcataag cgaacatttt
     6121 tatcgcccgt tgctctgctt ttgcggtgat ccctttttca gccatcaccc gacgatgact
     6181 caaaatcagt tttgacagcg gaatacgcac cggacacacg ctgtcacaag cagtgcataa
     6241 agagcaggcg tagggtaaat ctttaaaatc tttatagccg ccaagtagcg gagaaatcac
     6301 cgcaccaatt ggccctggat aaatagagcc atatccatga ccgccaatat ggcgatatgc
     6361 cggacaagta ttcatacaag ccccgcagcg aatacagcgc agcacatccc gaaattcaga
     6421 ggccagcacc tcagaacgcc cgttatcgac aataaccaga tgaaactctt caggaccatc
     6481 aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg tatcccgtca aacgtgcacc
     6541 aacggcactg cgcgccagca tggtgatcaa tacatctacc tcggcaaacg tgggggcaat
     6601 acgctccatt cccatcactg caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt
     6661 accttcattg gtcaccaggc ataccgaacc tgtctctgcc accgcgaaat tacagccggt
     6721 aatacctatt tcagcactga ggaaatcttc gcggattttt tgccggatga ataaggtcat
     6781 tgcttcaggc gtttccggcc cctcatagcc cagacgttcg tgtagcactc gacggatctg
     6841 atggcgatct ttatgaattg ccgggaccac aacatgagat ggcggatctt gatccagctg
     6901 gagaatatat tcacccagat cggtttcaat cacctgaatg ccagcatcct gcaatacatg
     6961 attgacacca atctcttcgg tcaccatcga tttagatttc accaccttcc gggcattttt
     7021 gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct ttggtttttg caaaccagac
     7081 gtgaccgccg ttttgcgtca ctttttctga gagctggtac agataagcgt cgagattact
     7141 cagaacatga tcacgtatct gggcagcccg atcgcgccac tcctcccagt gccccaattc
     7201 atcgaccatt ttttgccgat ttgccccaat acgctgctgc gcgtttgcca ccgctttgcg
     7261 catgatcgga tcttcaattt gctgacggat gcgtgtctta aaatctgtat tactggtttt
     7321 gatcgacatc tttatatcct cagcggctca tcaacacttc agcaatatgc atcactttga
     7381 ctttctgccc ttcccgttgt aatcgcccac tgatgttcag caggcaactc acgtcagcac
     7441 caattaaata ctcagggcgg acttccatca ggtgcgcaac cttttctttc accatctcgc
     7501 cggatatttc ggccattttg accgagaacg tgccgccaaa tccgcagcag gtatcctgtt
     7561 cagcaaaggt caacagctcc agtccacgca catttttcag cagcgtaagt ggttcgtcct
     7621 tcacccccag cttacgggcc aggctacaag atgggtgata caccgctctc ccttgcaaac
     7681 tggcacctac atcgactacc cctaatttat taacaataaa agaggtgaga tcctgcatac
     7741 gcgcggcaac cttttcggct cgtgatgccc attcaggttc atccgccaga tacgtcgggt
     7801 aactttttac ggcataggtg caagagccag ctggtgaaat aatgggatcg tcgttatcct
     7861 ccagtgcggc gatcagattt ttcatccctg gaatcgcttc tttgatataa ccgctattga
     7921 tcgcaggctg accgcagcat ccctgtttct ccgggaaatt tacgcgacag ccgagttttt
     7981 ccagtagcag cacggagtct cgtgccattc ttgatttcag ggcgtcacca atacaggtga
     8041 caaagaaatt gacattcaca actaatactc cattacttca tgcccattta tgcccgatgt
     8101 ttccccccca ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc
     8161 aacataccac ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa
     8221 taactaattt tatttctttc ggcatcgctc acattttatg gggaaatcga cgatcatttt
     8281 tggcataaga aatccattta aaaatggcca catcccttat acctattcag agctacttat
     8341 tcaggggtta ttgagatata cactgatggt gtacaaacaa aatcaccgac aataatcacg
     8401 tatcaagtaa tagtcggagt tttatttaat tgaagcaatt tagtttgcat ttttatttaa
     8461 aaaccgagct agagtccatc agaaaaaaaa tatataaagc ccaaaccacc gttggaaata
     8521 gaaatagctg aaaggctcac aacacccgga atgcggatta tctttgctcc gattcctggc
     8581 ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg ggtacaacca
     8641 aactcgcgga caaacgcctt gtgaaaagat gattcactgg catagcctac tgactcagcg
     8701 atcaccacaa cagggagcgt ttcccgggaa aacatctggg ccgctatttg tagacgcaac
     8761 tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg ggcaaaactt
     8821 gcccgggaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg tcctggcatt
     8881 tccaacattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact gagaatgttt
     8941 ttctcagtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa tgtagcgcag
     9001 atctggctac acagtgcatc cactcccggc attgccaatc tggattcctg ttgtaaaaac
     9061 gggatcgcct ccctcagcca gttatattct acgctgtggt taaccggtgc taaaaacagc
     9121 gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcaaccgaag agtgccgcag
     9181 acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt ttgtggtagc
     9241 aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc cagcttcgcc
     9301 gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc atgtggaagc
     9361 tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc gttaagcatc
     9421 aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta caaacaccaa
     9481 caatcttagt aaataactaa tctaatacca tcaaatagtt aatcatgatt tttgttttgt
     9541 gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag cgttgtgaag
     9601 aaatggtatt tgctcttttg tgtctatagt catgatgtca aatgaacgcg tttcgacagg
     9661 aaatcatcat gaataaatat caggcagtga ttattggttt tggcaaggct ggaaaaacat
     9721 tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa tcaaatgcaa
     9781 tgtatggcgg gacctgtatt aatatcggct gcatcccaac caaaacattg gttcatgacg
     9841 cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg gttaattttt
     9901 tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg atcgacggcc
     9961 aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggga aatctggaaa
    10021 ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg ccaattcctg
    10081 gaattaccac cacgcctggt gtatatgaca gcaccggatt acttaatcta aaagaattgc
    10141 ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc tctatgttcg
    10201 ctaattttgg cagcaaggta accattttag aagcagcttc gctgtttttg cctcgggaag
    10261 atcgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc gatattatcc
    10321 tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg catagcgagc
    10381 acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg gctaccgctt
    10441 cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt gtcgttgaca
    10501 agcaattaca taccaccgca gacaatattt gggcgattgg agatgttacc ggcgggctgc
    10561 aatttactta tatttcactg gatgattacc gcattgtgcg tgatgagttg ctgggagaag
    10621 gcagacgtag tactgatgat cggaaaaatg tgccttattc cgtattcatg acaccgccac
    10681 tgtccagggt tggtatgaca gaagaacagg ccagagagag tggtgctgat attcaggtgg
    10741 tgacattgcc tgtagctgca attccgcgtg ccagagtgat gaatgatact cgtggggtat
    10801 taaaagcgat tgttgataat aaaacccaac gtatattagg ggcatcactg ctgtgtgttg
    10861 actcccacga gatgatcaat atagtgaaaa tggtgatgga tgccgggctg ccttatagca
    10921 tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat gatctatttt
    10981 cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt ctcagagaaa
    11041 agaatcttat taattccctg ttactctata ac
//
LOCUS       DAPHDX010000138        10982 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPHDX010000138 DAPHDX010000000
VERSION     DAPHDX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.140"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            204..1418
                     /locus_tag="RH058_003710"
     CDS             204..1418
                     /locus_tag="RH058_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDV3139236.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1485..2768
                     /locus_tag="RH058_003711"
     CDS             1485..2768
                     /locus_tag="RH058_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3139237.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3020..3937
                     /gene="oxyR"
                     /locus_tag="RH058_003712"
     CDS             3020..3937
                     /gene="oxyR"
                     /locus_tag="RH058_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV3139238.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3920..5320)
                     /gene="sthA"
                     /locus_tag="RH058_003713"
     CDS             complement(3920..5320)
                     /gene="sthA"
                     /locus_tag="RH058_003713"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV3139239.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5651..6301
                     /gene="fabR"
                     /locus_tag="RH058_003714"
     CDS             5651..6301
                     /gene="fabR"
                     /locus_tag="RH058_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV3139240.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6301..6660
                     /locus_tag="RH058_003715"
     CDS             6301..6660
                     /locus_tag="RH058_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV3139241.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6700..7800)
                     /gene="trmA"
                     /locus_tag="RH058_003716"
     CDS             complement(6700..7800)
                     /gene="trmA"
                     /locus_tag="RH058_003716"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV3139242.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7914..8104
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8169..10013
                     /gene="btuB"
                     /locus_tag="RH058_003717"
     CDS             8169..10013
                     /gene="btuB"
                     /locus_tag="RH058_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV3139243.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9958..10815
                     /gene="murI"
                     /locus_tag="RH058_003718"
     CDS             9958..10815
                     /gene="murI"
                     /locus_tag="RH058_003718"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV3139244.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PYLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2526 a         2597 c         2938 g         2921 t
ORIGIN      
        1 gagtttgtta tgtgattatt taacgtgttg ttttaacgat ggtggttaat tattcaggtg
       61 gtttttgagg gtattttttc tcctcggcgg aaatttttat ttttaatgtg atcacggttg
      121 aataatcgct ctttctctct tgtatggtag taggcgtaaa attaagtttg aagcgtctat
      181 ttacaacaca ggagattgca ataatgaaaa ttattgctgc agatgttttt gttacttgcc
      241 cagggcgtaa tttcgttacg cttaaaataa ccactgaaag cggactttgt ggattaggag
      301 atgccacatt aaatggtcgc gaattgtcgg tcgcttctta tctgaaagat catctttgtc
      361 cacaactaat tggccgcgat gccagccgta tcgaagatat ctggcaattc ttttataaag
      421 gtgcttactg gcgacgtggc cccgtcacga tgtcagccat ttctgccatt gatatggcgt
      481 tgtgggatat caaagctaaa gcggcaaata tgccattgta tcaactgctt ggtggtgctt
      541 ctcgtgaagg cgtgatggtt tattgccaca ccaccgggcg gacgattgat gaagtccttg
      601 aagactatgc aaagcaccag cagatgggct ttaaggcgat tcgtgtgcag tgcggtgtac
      661 cgggtatgca aactacttat ggcctggcga agggcaaagg cctggcgtat gaaccggcaa
      721 ccaaaggcct gtggccagaa gagcagttgt ggtcttcaga aaagtatctc gattttacgc
      781 cgaagttatt tgaagcggtg cgtaataagt tcggttttaa cgaacatctg ttgcatgaca
      841 tgcaccatcg cctgaccccc atcgaagcgg cgcgctttgg gaagagcatt gaggattacc
      901 gcctgttctg gatggaagat ccgacgcctg cagagaacca ggaatgtttt cgtctgattc
      961 gccagcatac cgtgacgccg attgcggtcg gtgaagtgtt taacagtatc tgggactgca
     1021 agcagttaat cgaagagcag ctgattgact atattcgcac gacgatcacc catgcaggcg
     1081 gcattaccgg gatgcggcgg attgccgatt ttgcatcgct ttatcaggta cgtaccggtt
     1141 cgcatggccc ttcagattta tcgcctgttt gtcatgcggc ggcgctgcat tttgacctat
     1201 gggtaccaaa ttttggcgtt caggagtaca tgggctattc cgaacaaatg cttgaagttt
     1261 tcccgcacag ctggcgcttc gaggaaggtt atatgcaccc aggcgatgaa ccggggctgg
     1321 gtatttcttt cgatgagaaa ctggcggcga aatatccgta tgacccggcg tatctccccg
     1381 tcgcacgact ggaagatggc acattgtgga actggtaata acggttcgct taattatttt
     1441 ctgaatagtc atcaaatcga ttaattccgc tcaaggagct aaaaatggac gttgatgtgt
     1501 caaccagtgt tgcgggtaat aaaccgcaac ggattcgtcg tattcagaca gtgaccctgg
     1561 ttttattatt tatggcgggg atcgttaatt ttctcgaccg ttcgtcattg agcgtggcag
     1621 gtgaagcgat tcgtggcgag ttaggattat cggcgacaga gtttggtgtt ttgctttctg
     1681 cattttctct gtcttatggt ttttcacaac taccttccgg tattttgcta gaccgctttg
     1741 gcccacgaat tgttttaggc gcaggcttaa tattctggtc attaatgcag gcattaacag
     1801 gaatggttaa ttcttttagc cactttattt taatgcgtat cggtctgggt attggcgaag
     1861 cgccatttat gcctgcggga gtaaagtcga tcaccgactg gtatgcacaa aaagaacgcg
     1921 gcacggcgct ggggattttt aactcatcta ccgttatcgg tcaggctatc gcgcctcctg
     1981 ctcttgtatt gatgcagctg gcatggggct ggcggacgat gttcgttatc atcggcgtgg
     2041 cagggattct ggttgggatc tgttggtacg cgtggtatcg caaccgggcg cagtttgtcc
     2101 tgactgacga agaacggacg tatctctccg cgccggttaa accgcgtcca cagctgcaat
     2161 ttagcgagtg gctggcgctg tttaagcatc ggacaacctg ggggatgatt ttaggtttct
     2221 ctggcgtcaa ttataccggt tggctctaca tcgcgtggtt acccggttat ttgcaagccg
     2281 agcaaggttt cagtctggcg aaaaccggtt gggtggcggc gattcctttc ctcgcggcgg
     2341 cagtcgggat gtgggttaac ggtattgttg tcgatcgact ggcgaaaaaa ggctacgacc
     2401 tggcgaagac gcgtaaaacg gctattgtct gcggtttgat gatgtcggca ttaggcacgt
     2461 tgctggtcgt gcaatcttcc tcgccagccc aggcggtagc gtttatctca atggcgctat
     2521 tctgcgtgca tttcgctgga acgtctgcat gggggctggt gcaggtgatg gtgtcagaaa
     2581 caaaagtggc ttccatcgcc gggattcaaa actttggcag ttttgttttt gcttcctttg
     2641 ctccgatcgt aaccggttgg gtagtggata ccacacactc gtttaatctg gcgttggtta
     2701 tcgcggcctg tgtgacgttc accggggcgc tgtgttactt ctttattgtc aaagatcgca
     2761 ttgagtaatc gttactatca ggctctcttg ctgtgggcct gtagaataaa aaaaagcgga
     2821 tcctggagat ccgcaaaagt tcacgttggc tttagttatt cgagttgaga aactctcgaa
     2881 acgggcagtg acttcaaggg ttaaaagagg tgccgctccg tttctgtgag caattatcag
     2941 tcagaatgct tgatagggat aatcgttcat tgctattcta cctatcgcca tgaactatcg
     3001 tggcgatgga ggatggataa tgaatattcg tgatcttgag tacctggtgg cattggccga
     3061 gcaccgccat tttcggcgtg cggcagattc ctgccacgtt agccagccga cgcttagcgg
     3121 gcaaattcgt aagctggaag atgagttggg cgtgatgttg ctggagcgga ccagccgtaa
     3181 agtgttgttc acccaggcgg gaatgctgct ggtagatcag gcgcgtaccg tactgcgtga
     3241 ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag acgatgtccg ggccgctgca
     3301 cattggtttg attcccacag ttggaccgta cctgctaccg catattatcc ctatgctgca
     3361 ccagaccttt ccaaagctgg aaatgtatct gcatgaagca cagacccacc agttactggc
     3421 gcaactggac agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc
     3481 attcattgaa gtgccgttgt ttgatgagcc aatgttgctg gctatctatg aagatcaccc
     3541 gtgggcgaac cgcgaatgcg taccgatggc cgatctggca ggggaaaaac tgctgatgct
     3601 ggaagatggt cactgtttgc gcgatcaggc aatgggtttc tgttttgaag ccggggcgga
     3661 tgaagataca cacttccgcg cgaccagcct ggagacgctg cgcaacatgg tggcggcagg
     3721 tagcgggatc actttactgc cagcgctggc tgtgccgccg gagcgcaaac gcgatggggt
     3781 tgtttatctg ccgtgcatta agccggaacc acgccgcact attggcctgg tttatcgtcc
     3841 tggctcaccg ctgcgcagcc gctatgagca gctggcagag gccatccgag caagaatgga
     3901 tggccatttc gataaagtgt taaaacaggc ggtttaaacc gtttaacgca gctacccgat
     3961 aggcttccgc catggtcggg tagttaaagg tggtgttgac gaagtactca atagtgttgc
     4021 cgccaccttt ctgttccata atcgcctgac cgatatgaat aatttcggca gcgcgctcgc
     4081 caaagcagtg aatacccaga atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc
     4141 ccacgttcat gccgacgatt tgtgcgcgtg ccagatgttt aaactgggcg cggcccactt
     4201 catatggcac tttcatcgcg gtcagctgct gttcggtttt gcccacagag ctgatttccg
     4261 ggatggtgta aatgccggta gggatatctt caatcagatg cgcggtggct tcgcctttta
     4321 ccagcgcctg cgcggcaatg cgcccctggt cataggccgc tgatgccagg ctcggataac
     4381 caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct
     4441 tcagctgtcc gcgactgtca gtttccagcc caatgttctg taacgccagc gaatcggtat
     4501 taccggtacg accgttggca tagagcaggc agtcagcttt cagtttttta cccgacttca
     4561 gatgcatgat cacaccgtcg tcacagcctt cgatcttctc gtactcttcg ttgtgacgaa
     4621 tcactacgcc actgttccag aagtgatagg agagagaatc tgacatctct tgatcgagaa
     4681 atgccagcag gcgatcgcgg gtgttgatca gatccacttt tacatccata ccgcggaaga
     4741 tcgacgcata ttcacagccg atcactccag caccatagat aagtacatgg cgcggttcgt
     4801 ggtgcatgct aagaattgag tcgctgtcgt aaatgcgtgg atgggtgaaa tcaacatctg
     4861 ttggatgata tggacgagag ccgcaggcaa taacaaattt ttcagcggtt agtgtttcaa
     4921 cgctgccgtc cgggcaatcc agcgccaacg tatgctcgtc aacaaagcga gcgtttccct
     4981 gcaatatttc acagtgatta cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa
     5041 tcacgttatc ggcatggtta aggatatcgg caaaagaaga gcggagcagt cgggaatggt
     5101 cgctgtaaag tgggttttga ttgaattcta taatgcggct gacggcgtga cggagagctt
     5161 tcgacgggat ggtgccccag tgggtgcaac cgccgccaac attttgataa cgctcgataa
     5221 ctgcgacgcg cgcaccttgc ttaaccaggc ccattgcagc gccttcgccg ccggggccgg
     5281 aacctattac tatggcatcg taatcgtagg aatgtggcat ggtagggctt acctgttctt
     5341 atacataaaa gcaacagaat ggtaacattt tatcgcgggt aagccaattg atccccgtca
     5401 tttatctggc tatatcctga gcggcctttg ctttgtctgt ttcttacttt tgccctgacg
     5461 ttttattgga tttttatcga cgatactctc cgtttaagcg gcaggtttcc gctgtacgta
     5521 aaagaaccgg ccaaagaatt gcagtaaata tgttttattg cgttaccgtt cattcacaat
     5581 actggagcaa tccagtatgt tcattctctg gtatagtgcc agcagtactt ttggcaagga
     5641 ttcagacatc gtgatgggcg taagagcgca acaaaaagaa aaaacccgcc gttcgctggt
     5701 ggaagccgca tttagccaat taagtgctga acgcagcttc gccagcctga gtttgcgtga
     5761 agtggcgcgt gaagcgggca ttgctcccac ctctttttat cggcatttcc gcgacgtaga
     5821 cgaactgggt ctgaccatgg ttgatgagag cggtttaatg ctacgccaac tcatgcgcca
     5881 ggcgcgtcag cgtatcgcca aaggcgggag tgtgatccgc acctcggtct ccacatttat
     5941 ggagttcatc ggtaataatc ctaacgcctt ccggttatta ttgcgggaac gctccggcac
     6001 ctccgctgcg tttcgtgccg ccgttgcgcg tgaaattcag cacttcattg cggaacttgc
     6061 ggactatctg gaactcgaaa accatatgcc gcgtgcgttt actgaagcgc aagccgaagc
     6121 aatggtgaca attgtcttca gtgcgggtgc cgaggcgttg gacgtcggcg tcgaacaacg
     6181 tcggcaatta gaagagcgac tggtactgca actgcgaatg atttcgaaag gggcttatta
     6241 ctggtatcgc cgtgaacaag agaaaaccgc aattattccg ggaaatgtga aggacgagta
     6301 atgaaacaag caaatcaaga tagaggtacg ctgctgctgg cgttggttgc tggcttatcg
     6361 attaatggta ctttcgccgc gctgtttagc tccattgtgc cattttctgt attcccgatt
     6421 atttccctgg tgctgacggt ttactgcctg catcaacgtt atcttaatcg cactatgccg
     6481 gtaggcttgc cgggtctggc agctgcctgt tttattctcg gcgtactgct gtacagcacg
     6541 gtagttcgtg cggaatatcc ggatatcggc tctaacttct tcccggcagt actctccgtc
     6601 attatggtgt tctggattgg cgcgaagatg cgtaaccgta agcaggaagt tgctgagtaa
     6661 tcggtattat gccggatgct ggcgcatccg gcatgggttt tacttcgcgg tcagtaatac
     6721 gccgcactcc atatggtgcg tgtaggggaa ctgatcaaac agagccagac gttcgacctt
     6781 gtgcgtctgg cttaatgttt ccagattctt acataacgtt tccgggttac aggagatgta
     6841 caaaatacgc ggatacgcct gcaccatttt ctcggtttca ctgtccagac cgctgcgcgg
     6901 tgggtcgaca aaaatggttt cgcactgata actctttaag tcgatccctt gcaggcggtt
     6961 aaactcgcgc acaccattca tcgcctgagt aaattcttct gccgccatac gaataatttg
     7021 tacgttatca atatggttag ctgcgatgtt gtattgcgca gcagcaaccg acggcttagc
     7081 gatttcggtg gctaataccc gatcaaaatt gcgcgccagc gctaatgaaa agttaccgtt
     7141 gccgcagtac agctccagta aatcgccttt tgagcctttg gttacgtcca gcgcccattc
     7201 cagcatctga atattcatcg ccgcgttcgg ctgggtaaag ctgttttcta cctgacggta
     7261 gatcatctct ttccctgcga ccggcagacg ttcatcgatg taatcttgat ccagctcgat
     7321 tttggttttc gttgcccgac caatcagatg cacattcaga ttctgcgcgc gcagtgcatc
     7381 gcgcagggct tccgcttcct gacgccactc atcatccagc tttttatggt acaacaggga
     7441 aaccaccgcc tgattactca gcgtggtgag gtaatcaatc tggaacaact tgtggcgcag
     7501 aacgggatta ttacgcacac ccgcaatcat cgccgtcatc aactggttga tgagttcact
     7561 ggcagcgggg aagctatcca cgcggatgcg gcttttggtt tgttgatcga aaatgatgtg
     7621 atacaggtca tcgccatcgt gccagatgcg gaactccgca cgcatccggt aatgactgac
     7681 cggcgagcga aacacttccg gaaccaggtc agaaaacggt gccatcatac tttgcaaacg
     7741 taccactttt tcggctaact gcgcttcata ctgttctgtt ggaaggtgtt cgggggtcat
     7801 gatgtatcct gaaaaattaa agtacgcggc gattgtaggg attgctcatc agatgtccag
     7861 atcttgatga attcctattt gtgagctacg tctggacagt aacttgttac aacctgtagc
     7921 atccacttgc cggtcctgtg agttaatagg gaatccagtg cgaatctgga gctgacgcgc
     7981 agcggtaagg aaaggtgcga tgattgcgtt atgcggacac tgccatccgg tgggaagtca
     8041 tcatctctta gtatcttaga tacccctcca agcccgaaga cctgccggcc aacgtcgcat
     8101 ctggttctca tcatcgcgta atattgatga aacctgcggc atccttcttc tattgtggat
     8161 gctttacaat gattaaaaaa gcttcgctgc tgacggcgtg ttccgtcacg gccttttccg
     8221 cttgggcaca ggataccagc ccggatactc tcgtcgttac tgctaaccgt tttgaacagc
     8281 cgcgcagcac tgtgcttgca ccaaccaccg ttgtgacccg tcaggatatc gaccgctggc
     8341 agtcgacctc ggtcaatgat gtgctgcgcc gtcttccggg cgtcgatatc acccaaaacg
     8401 gcggttcagg tcagctctca tctattttta ttcgcggtac aaatgccagt catgtgttgg
     8461 tgttaattga tggcgtacgc ctgaatctgg cgggggtgag tggttctgcc gaccttagcc
     8521 agttccctat tgcgcttgtc cagcgtgttg aatatatccg tgggccgcgc tccgctgttt
     8581 atggttccga tgcaataggc ggggtggtga atatcatcac gacgcgcgat gaacccggaa
     8641 cggaaatttc agcagggtgg ggaagcaata gttatcagaa ctatgatgtc tctacacagc
     8701 aacaactggg ggataagaca cgggtaacgc tgttgggcga ttatgcccat actcatggtt
     8761 atgatgttgt tgcctatggt aataccggaa cgcaagcgca gacagataac gatggttttt
     8821 taagtaaaac gctttatggc gcgctggagc ataactttac tgatgcctgg agcggctttg
     8881 tgcgcggcta tggctatgat aaccgtacca attatgacgc gtattattct cccggttcac
     8941 cgttgctcga tacccgtaaa ctctatagcc aaagttggga cgccgggctg cgctataacg
     9001 gcgaactgat taaatcacaa ctcattacca gctatagcca tagcaaagat tacaactacg
     9061 atccccattt tggtcgttat gattcgtcgg cgacgctcga tgagatgaag caatacaccg
     9121 tccagtgggc aaacaatgtc atcgttggtc acggtagtat tggtgcgggt gtcgactggc
     9181 agaaacagac tacgacgccg ggtacaggtt atgttgagaa tggatatgat caacgtaata
     9241 ccggcatcta tctgaccggg ctgcaacaag tcggcgattt tacctttgaa ggcgcagcac
     9301 gcagtgacga taactcacag tttggtcgtc atggaacctg gcaaaccagc gccggttggg
     9361 aattcatcga aggttatcgc ttcattgctt cctacgggac atcttataag gcaccaaatc
     9421 tggggcaact gtatggcttc tacggaaatc cgaatctgga cccggagaaa agcaaacagt
     9481 gggaaggcgc gtttgaaggc ttaaccgctg gggtgaactg gcgtatttcc ggatatcgta
     9541 acgatgtcag tgacttgatc gattatgatg atcacaccct gaaatattac aacgaaggga
     9601 aagcgcggat taagggcgtc gaggcgaccg ccaattttga taccggacca ctgacgcata
     9661 ctgtgagtta tgattatgtc gatgcgcgca atgcgattac cgacacgccg ttgttacgcc
     9721 gtgctaaaca gcaggtgaaa taccagctcg actggcagtt gtatgacttc gactggggta
     9781 ttacttatca gtatttaggc actcgctatg ataaggatta ctcatcttat ccttatcaaa
     9841 ccgttaaaat gggcggtgtg agcttgtggg atcttgcggt tgcgtatccg gtcacctctc
     9901 acctgacagt tcgtggtaaa atagccaacc tgttcgacaa agattatgag acagtctatg
     9961 gctaccaaac tgcaggacgg gaatacacct tgtctggcag ctacaccttc tgaaccacgt
    10021 cccaccgtgc tggtgtttga ctccggcgtc ggtgggttgt cggtctatga cgagatccgg
    10081 catctcttac cgtatctcca ttacatttat gctttcgata acgtcgcttt tccgtatggc
    10141 gaaaaaagcg aagagtttat tgttgagcga gtggtggcaa ttgtcaccgc agtgcaagaa
    10201 cgttatcccc ttgcgctggc tgtggtcgct tgcaacaccg ccagtaccgt ttcacttcct
    10261 gcattacgcg aaaagttcga cttcccggtt gttggtgtcg tgccggcgat taaacctgct
    10321 gcacgtctga cggcaaatgg cattgtcgga ttactggcaa cccgcggaac tgttaaacgt
    10381 tcttatactc atgagctgat cgcgcgtttc gctaatgaat gccagataga aatgctgggc
    10441 tcggcagaga tggttgagtt ggctgaagcg aagctgcatg gcgaagatgt ttctctggat
    10501 gcactaaaac gtatcctacg cccgtggtta agaatgaaag agccgccaga taccgttgta
    10561 ttgggttgca cccatttccc tctactacaa gaagaactct tacaggtgct gccagaggga
    10621 acccggctgg tggattctgg cgcagcgatt gctcgcagaa cggcctggct gttagaacat
    10681 gaagctccgg atgcaaaatc tgccgatgcg aatattgcct tttgtatggc aatgacgcca
    10741 gaagctgaac aattattgcc cgttttacag cgttacggct tcgaaacgct cgaaaaactg
    10801 gcagttttag gctgatttgg ttgaatgttg cgcggtcaga aaattatttt aaatttcctc
    10861 ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca acggcaaaca
    10921 cgccgccggg tcagcggggt tctcctgaga attccgacag agaaagcaaa aataaatgct
    10981 tg
//
LOCUS       DAPHDX010000139        10930 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPHDX010000139 DAPHDX010000000
VERSION     DAPHDX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.141"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..805
                     /gene="adiY"
                     /locus_tag="RH058_003719"
     CDS             44..805
                     /gene="adiY"
                     /locus_tag="RH058_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV3139245.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            942..2279
                     /gene="adiC"
                     /locus_tag="RH058_003720"
     CDS             942..2279
                     /gene="adiC"
                     /locus_tag="RH058_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV3139246.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2383..4026
                     /gene="eptA"
                     /locus_tag="RH058_003721"
     CDS             2383..4026
                     /gene="eptA"
                     /locus_tag="RH058_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV3139247.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4023..4691
                     /gene="basR"
                     /locus_tag="RH058_003722"
     CDS             4023..4691
                     /gene="basR"
                     /locus_tag="RH058_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV3139248.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4692..5792
                     /gene="pmrB"
                     /locus_tag="RH058_003723"
     CDS             4692..5792
                     /gene="pmrB"
                     /locus_tag="RH058_003723"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV3139249.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5768..5857)
                     /gene="pmrR"
                     /locus_tag="RH058_003724"
     CDS             complement(5768..5857)
                     /gene="pmrR"
                     /locus_tag="RH058_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV3139250.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5969..7471)
                     /gene="proP"
                     /locus_tag="RH058_003725"
     CDS             complement(5969..7471)
                     /gene="proP"
                     /locus_tag="RH058_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV3139251.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7735..8613)
                     /locus_tag="RH058_003726"
     CDS             complement(7735..8613)
                     /locus_tag="RH058_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV3139252.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8610..10838)
                     /gene="crfC"
                     /locus_tag="RH058_003727"
     CDS             complement(8610..10838)
                     /gene="crfC"
                     /locus_tag="RH058_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV3139253.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRELTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2740 a         2850 c         2691 g         2649 t
ORIGIN      
        1 tttagcgaga actggtcttt tatttgcact caggagtaca tgtatgagga tttgcagcga
       61 ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga atgggaaaga
      121 acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata atattatcga
      181 cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca tcaaagatta
      241 cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta gcgctacgcc
      301 catactcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc aacacagcat
      361 gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat tcacggtgct
      421 atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt tacgtaattg
      481 tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact ggaatcttag
      541 tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagt tgaaaagcga
      601 aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg taaatgaatt
      661 aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca acagtacgtc
      721 gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt atgttagtca
      781 gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata ttaataacgg
      841 catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta tgatatgacg
      901 taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg gatgctgatg
      961 ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt atggggtcag
     1021 gtgtttttct gttacctgca aacctggcct ctactggcgg gattgccatt tatggatggt
     1081 tggtgacgat tatcggtgca ctggggctct cgatggtata cgccaaaatg tcgttcctcg
     1141 acccaagtcc tggtggttct tacgcttacg cccgccgctg ctttggcccg tttctcggtt
     1201 atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgcc atggtggtca
     1261 ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg gtattaacca
     1321 tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt ccgaaaatga
     1381 tcacccgtgt gcaggcagtt gccaccgtgc tggcgctgat ccccatcgtc gggattgccg
     1441 tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac gtcagcggcc
     1501 tgggcacctt cggtgcaatt caaagtaccc ttaacgttac gctgtggtcg ttcatcggtg
     1561 tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaat gtccctatcg
     1621 ccaccattgg tggggtattg attgccgccg tttgctatgt actttctacc accgcgatta
     1681 tggggatgat ccctaatgcc gcactgcgcg tttctgcttc gccattcggt gatgccgcac
     1741 ggatggcgtt gggtgacacc gccggggcca ttgtttcctt ctgcgcagct gcgggttgct
     1801 taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc gctgccgatg
     1861 acggactgtt cccaccaatt tttgcccgtg taaataaagc gggtacgcca gtggcggggc
     1921 tgattatcgt cggtattttg atgaccatct tccagctcag cagcatttca ccaaacgcga
     1981 ccaaagagtt cggtctggtt tcttccgtct cggtcatctt tacactggtg ccatatcttt
     2041 acacctgtgc ggcgttactg ctgctcggac acggtcactt tggtaaagca cgcccggcat
     2101 atctggcagt tactaccatt gccttcctct actgcatctg ggccgtggtg gggtccggag
     2161 cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat caccgccatg tatgcgctga
     2221 attacaaccg gctacataaa aacccgtatc ccttagatgc accaataagc aaagattaat
     2281 tccccttaat ccagcaaaca taaaagccaa ccttaagaac ttaaggttgg cttaattttg
     2341 ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa gcgcctacta
     2401 aaaagaccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta tatctctatc
     2461 tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga ttcgctgcat
     2521 aacgtactgg ttttcttgtc gatgccggtc gtcgctttca gcgtgattaa tattgtcctg
     2581 acactaagct ctttcttatg gcttaatcga ccactggcct gcctgtttat tctggttggc
     2641 gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc gatgattgcc
     2701 aatattattg ataccactcc ggcagaaagt tatgcgctga tgacaccgca aatgttatta
     2761 acgctgggat tcagcggcgt gcttgctgcg ctgattgcct gctggataaa aatcaaacct
     2821 gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt ttctgtacta
     2881 ctgattttgc tggtcgccgc actgttttat aaagactacg cctcgttgtt ccgcaataac
     2941 aaagagctgg tgaaatcctt aagcccctct aacagcattg ttgccagctg gtcatggtac
     3001 tcccatcagc gactagcaaa tctaccgctg gtgcgaattg gtgaagacgc ccaccgcaac
     3061 ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg cgaaacctcg
     3121 cgggcggaga acttctccct caacggctac ccgcgtgaaa ctaacccgcg gctggcgaaa
     3181 gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc agtttcagta
     3241 ccgtgcatgt tctcggatat gccgcgtgag cattacaaag aagagctggc acagcaccag
     3301 gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa tgacaacgat
     3361 ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc gctgaatctg
     3421 cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg gctggaagag
     3481 tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg cagccacggt
     3541 ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac ctgcgacacc
     3601 aatgagatcc agacctgtag caaagagcaa ctggtgaaca cttacgacaa cacgctggtt
     3661 tacgtcgact atattgttga taaagcgatt aatctgctga aagaacatca ggataaattt
     3721 accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa tggcatctat
     3781 ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc aatgctgctg
     3841 tggctgtcgg aggattatca aaaacggtat caggttgacc agaactgcct gcaaaaacag
     3901 gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg attaactggc
     3961 gttgagacga agtattacca ggctgcggat gatattctgc aaacttgcag gagagtgagt
     4021 gaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggattg attctggcgg
     4081 cgcaaaccga aggctacgcg tgcgatggcg tgacaaccgc gcggatggcg gaacaaagcc
     4141 ttgaggccgg tcattacagc ctggtggtac tggatttagg gttacccgac gaagatggac
     4201 tgcattttct cgcccgtatc cggcagaaaa aatacaccct gccagtactg atcctcaccg
     4261 ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt cggtgccgat gactatctgg
     4321 tgaagccttt tgcgctggaa gagttacacg cccgtatccg cgccctgcta cgacgccata
     4381 ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaatatg ggtcgccgtc
     4441 aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct ctgctgtcac
     4501 ggttaatgct caaagcaggc agtccggtgc atcgggaaat tctctacaac gacatctata
     4561 actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat ctgcgcgaca
     4621 aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctg gtcgcgaatg
     4681 aggaaaacta attgaatctg atgcattttc tgcgccgacc aatatcgctg cgccaacggc
     4741 tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc ttctggctat
     4801 ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac aatcgcaaca
     4861 acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt gtccccggcg
     4921 tgtttatggt cagcctgacg ctatttatct gctatcaggc ggtacgccgc atcacccgcc
     4981 cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc cgacaactta acgcccattg
     5041 ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttgaac gatctggtca
     5101 gtcgcctgac cagcacgctg gataacgaaa ggttgtttac cgctgacgtc gcgcacgaac
     5161 tgcgaacgcc actggctggg gtgcgtttgc atctggaact gctggcgaaa acgcatcaca
     5221 ttgatgtagc accgttagtg gcacggcttg atcagatgat ggagagcgtc tcccagctgc
     5281 tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat gtaaaactgc
     5341 tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac cagcgacagc
     5401 aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat gcaaccctgc
     5461 tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagcccg caaggcagca
     5521 acattatgat taagctgcaa gaagatggcg gagcggtcat ggcagttgaa gatgaaggac
     5581 caggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt atggacagcc
     5641 gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag ttgcatcacg
     5701 ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg gtacggctga
     5761 agaaagatca gaacgtggca aaccagatat aaagaaagct gctgaccacc agcacgctga
     5821 acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc gttaacctgg
     5881 aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc ctgataagac
     5941 agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt tgctgcacca
     6001 ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatattatcg attttctgct
     6061 cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct gacgccgccg
     6121 gtgtcgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg gtgattaaac
     6181 caatcaccgc cactaccatc aggtaatagg caggcatcat cagattctgc gagctttcga
     6241 ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata ttaaatgccg
     6301 ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta gaagccataa
     6361 cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa atcaggccga
     6421 tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagg gcaacactac
     6481 caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc accggctgga
     6541 caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct tcggagtaat
     6601 gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa gtcacgttgg
     6661 tggcaattac cagaccaata catgtcaaca ggctgcgcca gtgtttggtg gcaatctctt
     6721 taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc agtttatcga
     6781 catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc ccgataatcc
     6841 ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag ttcgcttcgc
     6901 cgacaatggt cgaaattaac accactacgc cagcacccag cacaaacccg gcaatagaac
     6961 caaagtccag ccagctgccc ataaagccac gtttacggtc aggggagtat tccgcaacaa
     7021 atatcgacgc cccggtatat tcaccgccga ccgagaaacc ttgtgccatc ttacagatca
     7081 acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag ccaatacaga
     7141 acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga ccatatttat
     7201 cgcccaacat accaaagaag agtccgccaa gcggtcgaat cagaaaggga acggagaacg
     7261 tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa actttaccta
     7321 atgcgtaagc aacaaaacca taaacaccaa aatcaaacca ttccattgca ttacccagtg
     7381 atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca tcacgaaggg
     7441 taatcggttt tacttttttc cttttcagca tagctttcct cgcagagttg gcccatttcc
     7501 gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt gcaggatgca
     7561 atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat gtacaatcaa
     7621 accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt gtttaaattt
     7681 gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga ttatttacgc
     7741 caaatttaca gagaagattt cctcgaccat tgcccccgcc agacgctgcg ctgaacagag
     7801 tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc actgaccagc aaacgggttg
     7861 tttatctgcc gtggtccact ttcccagcca acttaaaaaa tcagcatgat ccccgcccgt
     7921 cggaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtggtcg gggccgcatt
     7981 gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa tcacccagca acaacgccac
     8041 cgcttgcggg cttcccagac gacaggcatc gccaaatgcc ccccaacgca actcttcgag
     8101 aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt
     8161 aaaaatttgc tcgctctgtt cacccgcctg caccagtagg cctcgttgcg ttaatgcctt
     8221 ttccttccag cgacaacggg ctgaaaacca ctcatacaag gcggcaattt gctgggggag
     8281 ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca gatgctgcgc
     8341 gatctgcgtc agtgccagat gaccgggatc cagcatcgac gccagttttt ccaccaatgt
     8401 cagacgcagg ctaagcggcg catttaacat gccgccgttc gtccatgtct gaagctgcac
     8461 gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg cttgtggaag
     8521 gcgggcatca tcgccctgcg ccagatccac taaaaagcgg ggataaaccg actccagcac
     8581 gcgaccgggg ccgtcaagta acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga
     8641 atatcatcgc gtaacagctg ggcgtcggtg taaatccagc cgtgagtcat cacagtctgc
     8701 tgcaattgct gctgcatcag cctgaccacc gattcatttt gttgacgcag agccagactt
     8761 tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga actcagcgaa aaaagtattc
     8821 atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat ttgttggcaa
     8881 taaagatcga cttctgcgct taaagctcgt tgcaacacac tgtaatcaac cgtttctgtc
     8941 ggggatttct catttccccg tccccagtcg ggctgattca accagcgcga aaaagtctca
     9001 cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt cctggcgcga aatgacttca
     9061 ctgaacagct ggcgagtgtt gaagtgggga actacgccgt gaaaaacagg aaaatgaaac
     9121 ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat gggccgaata
     9181 acatcggtta actcccggca aagggtggat tcaagctcgg caaaacgcag cgtaaagtcg
     9241 cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcaggtacg aatctcgctt
     9301 aacggctccg aatcatcctg aaacaaggcg gctaactgcg cattcagcgc atcttgctgt
     9361 tgacgcagaa agtggttggc ggaggtcagg gccagctcga tttcatgttt aacctcgccg
     9421 ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg atggatattt
     9481 tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc
     9541 gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc gcaaagcata caacgaggcg
     9601 ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac
     9661 aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg acgccagcgc
     9721 ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cgctggtaac
     9781 ttaccgttgt tggctaactc atggcgcgcc cgattcgcca ggtagcccca catcgacgac
     9841 accggaaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga aatcagtgcc
     9901 cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca
     9961 tacagcggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc atcggagatc
    10021 gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg
    10081 ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt atccagtaac
    10141 gtcaactgac cgggataact ctccagcccc gccagatgga caaactccac ttcaatcacg
    10201 ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc
    10261 agtcgcacta aatcattcaa acttttcaga caatgaaaaa taggctgggc acccagataa
    10321 tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat atctttatct
    10381 atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt
    10441 tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca gtaccggttc cttttgcccg
    10501 ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattacgatt aggcagaacc
    10561 tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatggt accgacaatt
    10621 gccagcacca tttccagtcg ggaaatttta cgcaactcat tattcagcat cgcgtgacgt
    10681 tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcgtcgtc tccgggtaca
    10741 gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa caggcgttca
    10801 gcctcctgac ttaactcata cagggtctgt gtgtacatag aaaattcttc cttaaagcaa
    10861 attttgttac ttttatttag ccagattgtt tttgagttct gttttcggct tttataatta
    10921 ctgcaagaaa
//
LOCUS       DAPHDX010000140        10748 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPHDX010000140 DAPHDX010000000
VERSION     DAPHDX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.142"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            273..677
                     /gene="sdhC"
                     /locus_tag="RH058_003728"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             273..677
                     /gene="sdhC"
                     /locus_tag="RH058_003728"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV3139254.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            671..1018
                     /gene="sdhD"
                     /locus_tag="RH058_003729"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             671..1018
                     /gene="sdhD"
                     /locus_tag="RH058_003729"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV3139255.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1018..2784
                     /gene="sdhA"
                     /locus_tag="RH058_003730"
     CDS             1018..2784
                     /gene="sdhA"
                     /locus_tag="RH058_003730"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV3139256.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2800..3516
                     /gene="sdhB"
                     /locus_tag="RH058_003731"
     CDS             2800..3516
                     /gene="sdhB"
                     /locus_tag="RH058_003731"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV3139257.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3717..6518
                     /gene="sucA"
                     /locus_tag="RH058_003732"
     CDS             3717..6518
                     /gene="sucA"
                     /locus_tag="RH058_003732"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV3139258.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6533..7750
                     /gene="odhB"
                     /locus_tag="RH058_003733"
     CDS             6533..7750
                     /gene="odhB"
                     /locus_tag="RH058_003733"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV3139259.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     EFLEDPTRLLLDV"
     gene            7844..9010
                     /gene="sucC"
                     /locus_tag="RH058_003734"
     CDS             7844..9010
                     /gene="sucC"
                     /locus_tag="RH058_003734"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV3139260.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9010..9879
                     /gene="sucD"
                     /locus_tag="RH058_003735"
     CDS             9010..9879
                     /gene="sucD"
                     /locus_tag="RH058_003735"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV3139261.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(9983..10705)
                     /gene="mngR"
                     /locus_tag="RH058_003736"
     CDS             complement(9983..10705)
                     /gene="mngR"
                     /locus_tag="RH058_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDV3139262.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2529 a         2786 c         2972 g         2461 t
ORIGIN      
        1 ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg
       61 ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt
      121 tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc
      181 gcaagcagac cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt
      241 gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg
      301 tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga
      361 tagcgtccat tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc
      421 tgtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta
      481 tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg
      541 tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag
      601 cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag
      661 gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat
      721 cctcgttcgc gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt
      781 cgccaccagt ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac
      841 caaagtgttc accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg
      901 gcaggtgttg accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat
      961 tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg
     1021 aaattgccag tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc
     1081 gcggcgctgc aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg
     1141 acccgttccc ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat
     1201 gaagataact gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac
     1261 caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac
     1321 atgggcctgc cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt
     1381 cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc
     1441 ggtcacgcac tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc
     1501 tccgagtggt atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc
     1561 gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg
     1621 actggcgggg cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac
     1681 ggcgtcggca tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcagttc
     1741 cacccgaccg gtattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc
     1801 ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa
     1861 gacctggcgg gccgtgacgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc
     1921 ggctgtgatg gtccgtgggg gccacatgcg aaactgaaac tcgaccacct gggtaaagaa
     1981 gttctcgaat cccgtctgcc gggtattctg gaactctccc gtaccttcgc tcacgttgat
     2041 ccggtgaaag agccgattcc ggttatccca acctgtcact acatgatggg tggtattccg
     2101 accaaagtga ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg
     2161 gggctgtttg ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc
     2221 ggcaactcgc tgctggacct ggtggtcttt ggtcgcgcag cgggtctgca tctgcaagag
     2281 tctatcgccg agcagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg
     2341 gatcgtctga accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa
     2401 gcgctgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg
     2461 aaagggcttg agcagttgaa agttatccgc gagcgtctga aaaatgcccg tctggatgac
     2521 acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa
     2581 acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcgcatagc
     2641 cgcttcgact tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca
     2701 gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc
     2761 ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt
     2821 tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg
     2881 gaagaaggtc gcgacatgat gttgctggat gcgcttatcc agctgaaaga gaaagatccc
     2941 agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg
     3001 aacggcaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag
     3061 aagattgtga ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg
     3121 ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat
     3181 ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat
     3241 gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat
     3301 aagtttatcg gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat
     3361 accgagactg acagccgcct cgacggtttg agcgatgcat tcagtgtatt ccgctgtcac
     3421 agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc
     3481 catatcaagt cgatgttgtt gcaacgtaat gcgtaattaa atgttgatgc cggggaaatc
     3541 ccccggctat tcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt
     3601 tccttcgcga gccactacgt agacaagagc tcgcaagtga accccggcac gcacataact
     3661 gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc
     3721 agaacagcgc tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct
     3781 ggatagaaca gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc
     3841 gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa
     3901 cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg
     3961 accctgacac caatgtaaag caggttaaag tcctgcagct cattaacgca taccgcttcc
     4021 gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat aaagtggccg
     4081 atctggatcc gtctttccac gatctgaccg aagcagactt ccaggagacc ttcaacgtcg
     4141 gttcatttgc cagcggcaaa gaaaccatga aactcggcga gctgctggaa gccctcaagc
     4201 aaacctactg cggcccgatt ggtgccgagt atatgcacat taccagcacc gaagaaaaac
     4261 gctggatcca acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac
     4321 gcttcttaag cgaactgacc gccgctgaag gccttgaacg ttacctcggc gcaaaattcc
     4381 ctggcgcaaa acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga
     4441 tgatccgcca cgctggcaac agcggcaccc gggaagtggt tctcgggatg gcgcaccgtg
     4501 gtcgtctgaa cgtgttggtg aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt
     4561 ttgccggtaa acataaagaa cacctcggca cgggtgacgt gaaataccac atgggcttct
     4621 cgtctgactt ccagaccgat ggcggcctgg tgcacctggc gctggcgttt aacccgtctc
     4681 accttgagat tgtaagcccg gtcgttatcg gctctgttcg tgcccgtctg gacagacttg
     4741 atgagccgag cagcaacaaa gtgctgccaa ttaccatcca cggtgacgcc gcagtgaccg
     4801 gacagggcgt ggttcaggaa accctgaaca tgtcgaaagc gcgtggttat gaagttggcg
     4861 gtacggtacg tatcgttatc aacaaccagg ttggtttcac cacctctaat ccgctggatg
     4921 cccgttctac gccgtactgt actgatatcg gcaagatggt tcaggcaccg attttccacg
     4981 ttaacgcgga cgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta
     5041 acacctttaa acgtgatgtc ttcatcgacc tggtatgcta ccgccgtcac ggccacaacg
     5101 aagccgacga gccgagcgca acccagccgc tgatgtatca gaaaatcaaa aaacatccga
     5161 caccgcgcaa aatctacgct gacaagctgg agcaggaaaa agtggcgacg ctggaagatg
     5221 ccaccgagat ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtggtagcag
     5281 agtggcgtcc gatgaacatg cactctttca cctggtcgcc gtacctcaac cacgaatggg
     5341 acgaagagta cccgaacaaa gttgagatga agcgcctgca ggaactggct aaacgcatca
     5401 gcacggtgcc ggaagcggtt gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc
     5461 aggcgatggc tgccggtgag aaactgttcg actggggcgg cgcggaaaac ctcgcttacg
     5521 ccacgctggt tgacgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta
     5581 ccttcttcca ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgc
     5641 tgcaacacat ccataacggc cagggcgcgt tccgtgtttg ggactccgta ctgtctgaag
     5701 aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga accacgcacc ctgaccatct
     5761 gggaagcgca gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct
     5821 cctctggcga gcagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt
     5881 acgaagggca ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgtg
     5941 ctgagcaaaa catgcaggtt tgcgtaccgt ctaccccggc acaggtttac cacatgctgc
     6001 gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc
     6061 tgcgtcatcc gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag
     6121 ccatcggtga aatcgacgag cttgatccga agggcgtgaa gcgcgtagta atgtgttctg
     6181 gtaaggttta ttacgacctg ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca
     6241 ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc
     6301 agtttgctca cgtcaaggat tttgtctggt gccaggaaga gccgctcaac cagggcgcat
     6361 ggtactgcag ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg
     6421 caggccgccc ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc
     6481 aacaagatct ggttaatgac gcgctgaacg tcgaataaat aaaggataca taatgagtag
     6541 cgtagatatt ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg
     6601 gcataaaaaa cccggcgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga
     6661 caaagtggta ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga
     6721 tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc
     6781 cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca
     6841 ggcgtctctg gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc gcctgctggc
     6901 tgaacacaat ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgactcg
     6961 tgaagatgtg gaaaaacatc tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc
     7021 tgatccggcg gcgcaaccgg ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg
     7081 cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac
     7141 cacgttcaac gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc
     7201 gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt
     7261 tgaagccctg aaacgttacc cggaagtgaa cgcgtctatc gacggcgatg acgtggttta
     7321 ccacaactat ttcgacgtca gcatggcggt ttctacgccg cgcggcctgg tgacaccggt
     7381 actgcgtgat gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc
     7441 agtcaaaggc cgtgacggca agctgaccgt tgaagatctg accggtggta acttcaccat
     7501 caccaacggt ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag
     7561 cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga
     7621 gatcctgccg atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga
     7681 atccgtgggc ttcctggtaa cgatcaaaga gtttctggaa gatccgacgc gtctgctgct
     7741 ggacgtgtag tagtttaagc ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc
     7801 tacggtttaa aagataacga ttactgaagg atggacagaa cacatgaact tacatgaata
     7861 tcaggcaaaa caactttttg cccgctatgg cttaccagca ccggtgggtt atgcctgtac
     7921 tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggcccgt gggtagtgaa
     7981 atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa
     8041 agaagacatc cgtgcttttg cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac
     8101 agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg atatcgctaa
     8161 agagctgtat ctcggtgccg ttgtagaccg tagttcccgt cgcgtggtct ttatggcctc
     8221 caccgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcacc tgatccataa
     8281 agttgcgctt gatccgctga caggcccgat gccgtatcag ggacgcgagc tggcgttcaa
     8341 actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg gcctggcgac
     8401 cattttcctg gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca
     8461 gggcgatctg atttgcctcg acggcaaact gggcgctgac ggcaacgcac tgttccgcca
     8521 gcctgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc
     8581 acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc
     8641 aggtctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac cggctaactt
     8701 ccttgacgtt ggcggcggcg caaccaaaga acgtgtaacc gaagcgttca aaatcatcct
     8761 ctctgacgac aaagtgaaag ccgttctggt taacatcttc ggcggtatcg ttcgttgcga
     8821 cctgatcgct gacggtatca tcggcgcggt agcagaagtg ggtgttaacg taccggtcgt
     8881 ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg acagcggcct
     8941 gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg ccgcagtgga
     9001 ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca gggctttact
     9061 ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa aatggttggc
     9121 ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt caacaccgtg
     9181 cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc agcaccgttc
     9241 tgcaaagact ccattctgga agctattgat gcaggcatca aactgattat caccatcact
     9301 gaaggcatcc cgacgctgga tatgctgact gtgaaagtga aactggacga agccggcgtt
     9361 cgtatgatcg gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa aatcggtatc
     9421 cagccgggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc cggtacactg
     9481 acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac ctgtgttggt
     9541 atcggcggtg acccgatccc tggctctaac tttatcgaca ttctcgaaat gttcgaaaaa
     9601 gatccgcaga ccgaagcgat cgtaatgatc ggtgagatcg gcggtagcgc tgaagaagaa
     9661 gcggcagcgt acatcaaaga gcatgttacc aagccagttg tgggttacat cgctggtgtg
     9721 actgcgccga aaggcaaacg tatgggccac gcgggtgcca tcattgccgg tgggaaaggg
     9781 actgcggatg agaaatttgc tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg
     9841 gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc
     9901 cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat ggttggccat
     9961 cgtatgatgg ccttttttgt gcttatcgcg atgattttcg ttgcgctatc agggtaaatt
    10021 tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa cacccgacca tcaactaaat
    10081 atccacgcga tactttttca agaatcggct ttgtctggct gatattaagc agacggctca
    10141 tctcttcggt tggcatcaga ggaatgattt cctgttcgct acgatcgata accattttct
    10201 tcacttcttc aataaagtga tattttgaat tttccatgac ctgccaggtg agatccggga
    10261 acaacgcaag cggcatccag gtttcttcca gcgccattgg cttttgcttg cgatagcgca
    10321 cgcgcttcac atgccacaca cgatcctgcg cggtgatttg tagctgttgc tgaagaaaat
    10381 cgtcagccgg aatcacttca aatatcagga cttcactgtg tgtatcgacg tgacggtccg
    10441 acagtttttc atcaaaactg gttaactgaa aaatatcgta attgacccgc tcttctttga
    10501 cgtaagtccc gctgccctga atgctttcga ggatctgttg ctcgactaac tggcgcaaag
    10561 cctgacgcac cgtaacccgg ctgacgccaa actctgtttg taatgccgat tcggtaggta
    10621 acgcatcgcc aggtttaagc tcgccacgcg caatttgttc acgaatgcga tcggcaatct
    10681 gccggtataa gggcttgtgt cccattttta gtatctcatt aatacgaatt tagccattat
    10741 gcccgata
//
LOCUS       DAPHDX010000141        10701 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPHDX010000141 DAPHDX010000000
VERSION     DAPHDX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.143"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..612
                     /locus_tag="RH058_003737"
     CDS             46..612
                     /locus_tag="RH058_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV3139263.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            616..1191
                     /locus_tag="RH058_003738"
     CDS             616..1191
                     /locus_tag="RH058_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV3139264.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1233..2285)
                     /locus_tag="RH058_003739"
     CDS             complement(1233..2285)
                     /locus_tag="RH058_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV3139265.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDVLFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2510..3430
                     /gene="lpxL"
                     /locus_tag="RH058_003740"
     CDS             2510..3430
                     /gene="lpxL"
                     /locus_tag="RH058_003740"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV3139266.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3602..4828
                     /gene="mdtG"
                     /locus_tag="RH058_003741"
     CDS             3602..4828
                     /gene="mdtG"
                     /locus_tag="RH058_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV3139267.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4911..5285
                     /locus_tag="RH058_003742"
     CDS             4911..5285
                     /locus_tag="RH058_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV3139268.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5286..5513)
                     /locus_tag="RH058_003743"
     CDS             complement(5286..5513)
                     /locus_tag="RH058_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV3139269.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5686..8229)
                     /gene="mdoH"
                     /locus_tag="RH058_003744"
     CDS             complement(5686..8229)
                     /gene="mdoH"
                     /locus_tag="RH058_003744"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV3139270.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8222..9757)
                     /gene="mdoG"
                     /locus_tag="RH058_003745"
     CDS             complement(8222..9757)
                     /gene="mdoG"
                     /locus_tag="RH058_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV3139271.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10151..>10701
                     /locus_tag="RH058_003746"
     CDS             10151..>10701
                     /locus_tag="RH058_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3139272.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKKNKKFSMVKLSVIF"
BASE COUNT         2644 a         2786 c         2628 g         2643 t
ORIGIN      
        1 gacacgccat tttctttctc tcgcaaattc gttttggatg cacttatgtc attcacaaat
       61 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
      121 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
      181 cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt
      241 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
      301 actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc
      361 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
      421 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
      481 ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag
      541 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
      601 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
      661 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
      721 gttaatttcc gcatccagca ccttggctat agctggttat acggcacctt taaagatttc
      781 gacggtactt ttacctttga cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt
      841 aacaccacca gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcagat
      901 ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
      961 ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg
     1021 ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc
     1081 gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct
     1141 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agcgtgaaat
     1201 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt
     1261 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
     1321 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa
     1381 caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg gcagccatca
     1441 tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc
     1501 tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
     1561 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc
     1621 cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
     1681 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
     1741 ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact
     1801 tcatactcat agtggttacg catgtcgata aatagtacat cgggatcgtc aagcatggcg
     1861 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
     1921 ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg
     1981 tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc
     2041 tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
     2101 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
     2161 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
     2221 tcgctctcag ccaacatttt ggcttttagc gcgtcgttgg aaatgcggtt gtgtaacact
     2281 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
     2341 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
     2401 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
     2461 atgcgacaat acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct
     2521 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
     2581 cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt
     2641 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
     2701 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
     2761 atccgttggc atgggcctga tggaaaccgg catggcgtgg ttctggccgg accgccgaat
     2821 cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
     2881 cggcatcctg ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg
     2941 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
     3001 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
     3061 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg
     3121 cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg
     3181 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
     3241 gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc cgccactgga
     3301 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
     3361 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
     3421 acgctattaa atctcccctg ccggatgctt cagaatggca tccggcatta ccacagcaaa
     3481 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
     3541 ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
     3601 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
     3661 gctaggctgt tttcttaccg gtgccgcctt cagtctggta atgcccttcc tacccctcta
     3721 cgttgagcag cttggagtta ccggtcactc cgccctgaat atgtggtccg gtattgtctt
     3781 cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg
     3841 taaaggccga aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt
     3901 gatgggactg gcacaaaata tctggcagtt tttgatccta cgggcgcttc ttgggttact
     3961 tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag
     4021 cggctgggcg ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc tcggcccaat
     4081 ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag
     4141 tgtgctcata ctctgctttt tcgtcaccct gttctgcatc agagaaaaat tccagccggt
     4201 cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact
     4261 ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacaggct caattgcccc
     4321 cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag
     4381 tggcatgatc gcctcggtgc caggcgtggc ggctctgcta agcgcaccac gactcggcaa
     4441 acttggcgat cgaatcggac ccgaaaagat cctgattaca gcgctgatct tttctgtact
     4501 gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt
     4561 gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc
     4621 gagcaaccag atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg atattggcaa
     4681 cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt
     4741 tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg
     4801 tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga
     4861 atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt
     4921 acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg
     4981 gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg
     5041 acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt
     5101 tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag
     5161 agatacgcca ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc
     5221 agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac
     5281 gttaatcact catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc
     5341 agagagaatg gcagatcaag gatcgtgaca taacgccagg cggagtcacg cacatcccat
     5401 tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg actaataatg
     5461 ctgccacagc cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac
     5521 ctctttcgta aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc
     5581 ggatgcggcg tgaacgcctt atccgaccca caaaatcttg caaaatcaat aaattgcagg
     5641 aacgatgtag gcctgataag cgtagcgcat caggcaacta cgtttttatt gcgaagccgc
     5701 atccggttta cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga
     5761 agaatatctc tccggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc
     5821 gcttagcagc accaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg
     5881 ttcaacgtgg cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc
     5941 cattgcggtt gccagagcgt taaatgacgg gttaaacact gcgtgcataa agccatcatc
     6001 aagggagcgt tgacgattca tctcaaggaa ccgatcggta tcaaccagca cctgcggcgg
     6061 cgaatactct tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc
     6121 acggctggaa atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag
     6181 ccagaacagg aaacgcagat ccagccacgc catcccaaca gcccacacta accctaacag
     6241 cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg
     6301 cggtgaattc cacaccactt cccagccaag gaacgcgctg acaacgaaga ccgtatggaa
     6361 cagcatgcgt accggagcca gcagcacgga aaaaagcact tccagcagca acgataatgt
     6421 aacgcgccag aagccgccgt attctttcgt tcctttgcac cagataagca aaatgctcaa
     6481 taacttcggc aggaacaaca gcaccatagt cgaagcaaaa agtgcaatcg ccagctcagg
     6541 acgccactgc ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt
     6601 caacgcatgt actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc
     6661 ggagagataa gacatcacgc ccgtcaggaa caccgcacgg tgaaccgggt gcataccctt
     6721 aaccaggaac agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag
     6781 ctcatcaagc aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca
     6841 gaccccccaa cctgcacggc gcatcaacgc cgcttccacg aagtcatgtg acaggattga
     6901 accggcaaag gaaccttcgc ccggcagcgg agccagtgcg cagtgctcga taaacggttt
     6961 cacgcggata atcgcgttat gtccccagta gtgcgactcg ccaagttgcc agaagtgcaa
     7021 accggctgta aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gcgcatacag
     7081 cgtatccata ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc
     7141 catcaggcgc accagcccgc acaaacaatc accggtcatt accgagtcag catccagcac
     7201 caccatgtag ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt
     7261 acgcttcacg cggcgacggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc
     7321 gataagctcc atccaggctt tttgctctgc gacgcagata tccgggttat aactgtcact
     7381 aagaatgtag acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc
     7441 acgcaggcca gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt
     7501 gcgatgctcc gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc
     7561 gcgaccaata agcagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca
     7621 acagaacagt accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg
     7681 cataaaggaa acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc
     7741 ctgataagga agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag
     7801 gatcaacaga atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg
     7861 ctcttctttg gtcaaacgag ccagatagcg cggcgtgaca tcgcgtccac gcaggcgatc
     7921 ccagaaacgg cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc
     7981 ttctggcatc gccttcagct gatcgcgccc ttcgtcgtct ttaattaact gtccatcagc
     8041 aagtgaatct ggccaggctt gttccagacg cgcctttacc gagccttgcg gggaatcatc
     8101 ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt
     8161 cttcggcaat gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt
     8221 cttattcatt ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca
     8281 ccagcgcagc acgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca
     8341 gacgccagcc tttggtaacc gggttatagc gcaccgtgct ttcaactatc tcaccattat
     8401 caccaatgct ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc
     8461 cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct
     8521 tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt
     8581 tgtcttcatc acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg
     8641 gcagctgatc cggcgtccag taagcgacga tgttatcgtt ggtttcatcg ttggttggaa
     8701 tttctaccag ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg
     8761 gacgaagatc gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca
     8821 acagaccgaa gccttgcggg ttttccatag agaagctgct gaccgctaaa tgtttcgggt
     8881 tattcaacgg acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt
     8941 cgtgcaactc cggacgatag ttatttgcag gcgacggttg gttcggccca aacaggaaca
     9001 tactggttaa cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact
     9061 gcacatccac aaccgtgtca cgccctggca taaccacgaa tttgtaagca cctgtcgcgc
     9121 gcggcgagtc aagcaatgca taaatggtta aacgtttatc agtcggtttt ggacgctcga
     9181 tccagaactc tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca
     9241 ggccgcgggc agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc
     9301 cgagcatgct gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa
     9361 aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga
     9421 aataatccgg gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga
     9481 ccggggtatc gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat
     9541 tgttccagta cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat
     9601 cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt
     9661 gagcttgctt tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta
     9721 acattactgc agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta
     9781 gtaagcactt ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc
     9841 cattccgaca acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga
     9901 ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg
     9961 gtttgccagc aattttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta
    10021 gcgatgatgc tatgcataaa aaccttgaag cctgcgcctg gcgcggtcag gtcggtgtcg
    10081 ctatggcaac aagcctaaag gcataacccg acagggataa gacgaaaaat ccgagattag
    10141 ttaaccatat atgaatccag tacccgcgca acgtgaatat ttcctcgact ccatccgcgc
    10201 ctggctgatg ttgttaggga tcccttttca tatttcttta atctattcga gccatacatg
    10261 gcatgtgaat agcgccgaac catcattgtg gctgaccctt tttaatgact tcatccactc
    10321 gttccgcatg caggtatttt tcgttatatc cggctacttt tcctacatgc tttttttacg
    10381 ctatcccttg aaaaaatggt ggaaagtacg tgtcgaacgt gtaggtatcc cgatgttaac
    10441 agccatcccc ctactgacat taccgcaatt tattatgctg caatatgtca aaggaaaagc
    10501 ggaaagttgg cctgggctgt cattgtatga caaatataat acgttggcct gggaattaat
    10561 atcacacctg tggtttttac tggtgttagt agtcatgacg acgctgtgcg tatggatatt
    10621 taagcgcatc agaaataatt tagaaaattc tgataaaaag aataaaaaat tctcgatggt
    10681 aaaactatcg gtgatttttt t
//
LOCUS       DAPHDX010000142        10633 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPHDX010000142 DAPHDX010000000
VERSION     DAPHDX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.144"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..1127
                     /locus_tag="RH058_003747"
     CDS             3..1127
                     /locus_tag="RH058_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3139273.1"
                     /translation="MIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSA
                     KNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAA
                     LFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQL
                     GYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNS
                     GWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADL
                     NGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFAR
                     RKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1134..1898)
                     /gene="glcC"
                     /locus_tag="RH058_003748"
     CDS             complement(1134..1898)
                     /gene="glcC"
                     /locus_tag="RH058_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV3139274.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2149..3648
                     /gene="glcD"
                     /locus_tag="RH058_003749"
     CDS             2149..3648
                     /gene="glcD"
                     /locus_tag="RH058_003749"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV3139275.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3648..4700
                     /gene="glcE"
                     /locus_tag="RH058_003750"
     CDS             3648..4700
                     /gene="glcE"
                     /locus_tag="RH058_003750"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV3139276.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4711..5934
                     /gene="glcF"
                     /locus_tag="RH058_003751"
     CDS             4711..5934
                     /gene="glcF"
                     /locus_tag="RH058_003751"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV3139277.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTLVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5939..6343
                     /locus_tag="RH058_003752"
     CDS             5939..6343
                     /locus_tag="RH058_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV3139278.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6365..8536
                     /gene="glcB"
                     /locus_tag="RH058_003753"
     CDS             6365..8536
                     /gene="glcB"
                     /locus_tag="RH058_003753"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV3139279.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8892..10574
                     /gene="glcA"
                     /locus_tag="RH058_003754"
     CDS             8892..10574
                     /gene="glcA"
                     /locus_tag="RH058_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV3139280.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2465 a         2692 c         3003 g         2473 t
ORIGIN      
        1 tgatgattaa aattgaaaaa gtaattaata aaaatgacct taaggcattt atcgcttttc
       61 cgtcatcact ttatcccgac gatccaaact ggataccccc tttattcata gaacgcaacg
      121 agcatttgtc tgcgaaaaat ccggggacgg accatatcat ctggcaggcg tgggtggcaa
      181 aaaaagcggg gcagatagtt gggcgtatta ccgcgcaaat agataccttg caccgtgagc
      241 gttacggtga agataccggt catttcggca tgattgacgc cattgatgat ccgcaggttt
      301 ttgccgcgtt gtttggcgca gcggaagcgt ggttgaagtc acaaggtgca agtaagatca
      361 gcggtccttt cagcctgaat atcaatcagg aaagcggatt actgattgaa ggttttgaca
      421 caccaccctg tgcgatgatg ccacacggca aaccgtggta tgccgcgcat attgaacaac
      481 tgggttatca caaaggcatt gatttactgg cgtggtggat gcagcgaacc gatctcactt
      541 tctctccggc gctaaaaaaa ctgatggatc aggtgcgcaa aaaggtgacc attcgctgca
      601 tcaatcgtca gcggtttgcc gaagaaatgc agatcctgcg tgagattttc aactctggct
      661 ggcagcacaa ctggggattt gtgccgttta ccgaacatga attcgcgacc atgggcgatc
      721 aacttaaata tctggtgccg gacgatatga tctatatcgc tgagattgat tctgcaccct
      781 gcgcgtttat tgtcggcttg ccgaacatca acgaggcgat tgccgatctg aacggatcgc
      841 tctttccctt cggctgggca aaattgctgt ggcgtttgaa agtcagcggt gtgcgaactg
      901 cgcgagtacc gctgatgggc gtgcgcgacg agtatcagtt cagccgcatc ggcccggtga
      961 ttgcgctgtt attgattgaa gccttacgcg atccgtttgc ccgccggaag atcgatgcgc
     1021 tggagatgtc atggatcctc gaaaccaata caggcatgaa taacatgctg gaacgcattg
     1081 gggcggagcc gtacaagcgt taccgattgt atgaaaagca gatttaatac cagctaactc
     1141 aggttcatct ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg
     1201 tggagattct ttttcacggt ccgcacatga tcgcgtgctg cgcgctgggc gacgtgcggc
     1261 agccgctgca acaccgcgtt gtagatcctc gcatgctggc gatcgatctg ctttttttgc
     1321 tgtggtcgat ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc
     1381 aatgattgca gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg
     1441 aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct ctttgttgtt ctcactggcg
     1501 gcgagcattt tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt
     1561 gccgccagcc ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc
     1621 gttcgcggct gcgtactgaa cagatggatc agcgggctgg tgtcctgcac ccgattaagc
     1681 cgtgcgacac gagaatcgcg accctgcgcc gtctcaataa tcccgcgccc gcgcagcacg
     1741 gtcagccctt cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc
     1801 gagggaagtg gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct
     1861 gcaaccactt cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta
     1921 ggaccaattt ttctgttctt aactcgcaaa acacgcacat cacgtaagtg attatgttac
     1981 atcaatttaa cattgagtta accaaggcaa ggtcacagag ctggaaaaaa aatggtctga
     2041 ccggtagcta aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca
     2101 gcgcgcaaaa atcagctgcc acacaacaca acaaagcgaa gcctactcat gagcatcttg
     2161 tacgaagagc gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt actgatggca
     2221 ctgcgtgagc atgtccctgg acttgagatc ctgcataccg atgaggagat cattccttac
     2281 gagtgtgacg ggttgagcgc gtatcgcacg cgtccattac tggttgttct gcctaagcaa
     2341 atggaacagg tgacagcgat tcttgctgtc tgccatcgcc tgcgtgtacc ggtggtgacc
     2401 cgtggtgcag gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg
     2461 gtgatggcgc gctttaaaga gatcctcgac attaaccccg ttggtcgccg cgcgcgcgtg
     2521 cagccaggcg tgcgtaacct ggcgatctcc caggccgttg caccgcataa tctctactac
     2581 gcaccggacc cttcctcaca aatcgcctgt tccattggcg gcaatgtggc ggaaaatgcc
     2641 ggcggcgtcc actgcctgaa atatggtctg accgtacata acctgctgaa aattgaagtg
     2701 caaacgctgg acggcgaggc gctgacgctg ggatcggacg cgctggattc acctggtttt
     2761 gacctgctgg cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg
     2821 gtaaaactgc tgccgaagcc gcccgtggcg cgggttctgt tagccagctt tgactcggta
     2881 gaaaaagccg gacttgcagt tggtgacatc atcgccaatg gcattatccc cggcgggctg
     2941 gagatgatgg ataacctgtc gatccgcgcg gcggaagatt ttattcatgc cggttatccc
     3001 gtcgacgccg aagcaatttt gttatgcgag ctggacggcg tggagtctga cgtacaggaa
     3061 gactgcgagc gggttaacga catcttgttg aaagcgggcg cgactgacgt ccgtctggca
     3121 caggacgaag cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt cccggcggta
     3181 ggacgtatct ccccggatta ctactgcatg gatggcacca tcccgcgtcg cgccctgcct
     3241 ggcgtactgg aaggcattgc ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc
     3301 tttcatgccg gagatggcaa catgcacccg ttaatccttt tcgatgccaa cgaacccggt
     3361 gaatttgccc gcgcggaaga gctgggtggg aagatcctcg aactctgcgt tgaagttggc
     3421 ggcagcatca gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag
     3481 ttcaacagcg atgaaatcac gaccttccat gcggtcaagg cggcgtttga ccccgatggt
     3541 ttgctgaacc ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt tggtgccatg
     3601 catgtgcatc acggtcattt acctttccct gaactggagc gtttctgatg ctacgcgagt
     3661 gtgattacag ccaggcgctg ctggagcagg tgaatcaggc aattagcgat aaaacgccgc
     3721 tggtgattca gggcagcaat agcaaagcct ttttaggtcg ccctgtcacc gggcaaacgc
     3781 tggatgtgcg ttgtcatcgc ggcattgtta attacgaccc gaccgagctg gtgataaccg
     3841 cgcgtgccgg aacgccgctg gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc
     3901 ttccctgcga gccgccgcat tatggtgaag aagccacctg gggtgggatg gtcgcctgcg
     3961 ggctggcggg gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc
     4021 gcatcattac cggcgctgga aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg
     4081 ccggatacga tctctcacgg ttaatggccg gaagctacgg ttgtcttggc gtgctcactg
     4141 aaatctcgat gaaagtgtta ccgcgaccgc gcgcctccct gagcctgcgt cgggaaatca
     4201 gcctgcaaga agccatgaac gaaatcgccc agtggcaact ccagccgcta cccattagtg
     4261 gcttatgtta cttcgacaat gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa
     4321 aagcggcgcg tgaactgctg gggggcgaag aggttgcggg tcagttctgg cagcaattgc
     4381 gtgaacaaca actgccgttc ttctcgttac caggtacctt atggcgcatt tcattaccca
     4441 gcgatgcgcc gatgatggat ttacccggcg agcaactgat cgactggggc ggagcgttac
     4501 gctggctgaa atcgacagcc gaggacaatc aaatccatcg catcgcccgc aacgctggcg
     4561 gtcatgcgac ccgctttagt gccggagatg gtggctttgc cccgctatcg gctcctttat
     4621 tccgctatca ccagcagctt aaacagcagc tcgacccttg cggcgtgttt aaccccggtc
     4681 gcatgtacgc ggaactttga ggagcaggct atgcaaaccc aattaactga agagatgcgg
     4741 cagaacgcgc gcgcgctgga agccgacagc atcctgcgcg cctgtgttca ctgcggattt
     4801 tgtaccgcaa cctgcccaac ctatcagctt ctgggcgatg aactggacgg gccgcgcggg
     4861 cgcatctatc tgattaaaca ggtgctggaa ggcaacgaag tcacgcttaa aacacaggag
     4921 catctcgatc gctgcctcac ttgccgcaat tgtgaaacca cctgtccttc tggtgtgcgc
     4981 tatcacaatt tgctggatat cgggcgtgat attgtcgagc agaaagtgaa acgcccactg
     5041 ccggagcgaa tgctgcgcga aggattgcgc caggtagtgc cgcgtccggc ggtcttccgt
     5101 gcgctgacgc tggtagggct ggtgctgcga ccgtttttac cggaacaggt cagagcaaaa
     5161 ctgcctgctg aaacggtgaa agctaaaccg cgtccgccgc tgcgccataa gcgtcgggtt
     5221 ttaatgctgg aaggctgcgc ccagcctacg ctttcgccca acaccaacgc ggcaactgcg
     5281 cgagtgctgg atcgtctggg gatcagcgtc atgtcagcta acgaagcagg ctgttgtggc
     5341 gcggtggact atcatcttaa tgcgcaggag aaagggctgg cacgggcgcg caataatatt
     5401 gatgcctggt ggcccgcgat tgaagcaggt gccgaggcaa ttttgcaaac cgccagcggt
     5461 tgcggcgcgt ttgtcaaaga gtacgggcag atgctgaaaa acgatgcgtt atatgccgat
     5521 aaagcgcgtc aagtcagtga actggcggtc gatttagtcg agcttctgcg cgaggaaccg
     5581 ctggaaaaac tggcagttcg cggcgataaa aaactggcct tccactgccc gtgtacccta
     5641 caacatgcgc aaaagctgaa cggcgaagtg gaaaaagtgt tgcttcgtct tggatttacc
     5701 ttaacggacg ttcccgacag ccatctgtgc tgcggttcag cgggaacata tgcgttaacg
     5761 catcccgatc tggcacgcca gctgcgggat aacaaaatga atgcgctgga aagcggcaaa
     5821 ccggaaatga tcgtcaccgc caacattggt tgccagacgc atctggcgag cgccggtcgt
     5881 acctctgtgc gtcactggat tgaaattgta gaacaagccc ttgaaaagga ataaaaaaat
     5941 gaaaactaaa gtcattctta gccagcaaat ggcgagtgca attattgccg caggtcagga
     6001 agaggcgcag aaaaataact ggtctgtttc cattgctgtt gccgatgacg gcggtcatct
     6061 gctggcgtta agtcgcatgg acgattgcgc gccgattgcg gcttacatct cccaggagaa
     6121 agcgcgtacc gccgcgctgg ggcgtcgtga aactaagggc tatgaagaga tggtgaacaa
     6181 cggacgtacc gcgttcgtga ctgcgccgtt actgacgtcg ctggaaggtg gcgtaccggt
     6241 tgttgttgat gggcaaatta ttggtgccgt gggcgtttct ggtttaaccg gagcacagga
     6301 tgcgcaggtc gcgaaagcgg cagcagcggt gttggcgaaa taagcgaaaa cgaggagata
     6361 aacaatgagt caaaccataa cccagggccg tttacgcatt gacgccaatt ttaaacgttt
     6421 tgtggatgaa gaagttttac cgggaacagg gctggacgct gcggcgttct ggcgcaattt
     6481 tgatgaaatc gttcacgatc tggcaccaga aaatcgtcag ttgctggcag aacgcgatcg
     6541 cattcaggcg gcgcttgatg agtggcatcg cagcaatccg gggccggtaa aagataaagc
     6601 ggcctataaa tctttcctgc gtgaactggg ctacctggtg ccgcaaccgg agcgcgtgac
     6661 ggtggaaacc acgggcattg acagcgaaat caccagtcag gcggggccac agctggtggt
     6721 tccggcaatg aacgcccgct acgcgctgaa cgcggcgaac gctcgctggg gctcactgta
     6781 cgatgcgtta tacggcagcg acatcatccc gcaggaaggg gcgatggtca gcggctacga
     6841 tccgcaacgc ggtgagcagg ttatcgcctg ggttcggcgt ttcctcgatg aatctctacc
     6901 gctggaaaat ggcagctatc aggatgtggt ggcgtttaag gtggtcgata aacaattacg
     6961 catccagttg aaaaatggta aagaaaccac gttacgtacc ccggcgcagt ttgtcggtta
     7021 ccgtggcgat gccgctgcgc tgacctgcat tttgctgaaa aataacggcc tgcatattga
     7081 actgcaaatt gatgccaacg ggcggattgg caaagacgat ccggcgcaca tcaacgatgt
     7141 tatcgtcgaa gcggctatca gtaccattct cgactgcgaa gattcggtcg cggcggttga
     7201 tgcggaagat aaaatcctgc tgtaccgcaa tctgctgggc ctgatgcagg ggactctgca
     7261 agagaaaatg gagaaaaacg gtcgacaaat cgtgcgtaaa ctgaatgacg atcgtcatta
     7321 caccgccgcc gatggctctg aaatttctct gcacggacgc tcgctgctgt ttatccgcaa
     7381 cgtgggtcat ttgatgacca ttcctgtgat ttgggacagc gaaggcaatg aaatcccgga
     7441 aggcattctt gatggcgtca tgactggcgc gatcgccctc tatgatttaa aagtgcagaa
     7501 aaactcgcgc actggcagcg tctatattgt taaaccgaaa atgcacggtc cgcaggaagt
     7561 ggcgttcgcc aacaaactgt ttacccgcat tgagacaatg ctcggtatgg caccgaatac
     7621 cctgaaaatg ggcattatgg atgaagaacg tcggacctcg ctgaacttgc gtagctgtat
     7681 cgctcaggcg cgcaatcgcg tggcgttcat caataccggt ttcctcgacc gtaccggcga
     7741 tgaaatgcat tcggtgatgg aagctggccc gatgctgcgt aaaaaccaga tgaaatcgac
     7801 gccgtggatc aaagcctacg agcgtaataa cgtgctttcc ggtctgttct gtgggctgcg
     7861 cggtaaagcg caaattggta aaggcatgtg ggcaatgccg gacctgatgg cagacatgta
     7921 cagccagaag ggcgaccaac tgcgtgccgg ggcaaacaca gcctgggttc cgtcaccaac
     7981 cgctgctacg ctccatgcgc tgcactacca ccaaaccaac gtacagagcg tgcaagccaa
     8041 cattgcccag accgagttca atgctgaatt tgaaccgctg ctggacgatc tgctgactat
     8101 tccggtggct gaaaacgcta actggtcggt ggaagagatc caacaagagc tggataacaa
     8161 cgtgcagggg attctggggt acgtggtgcg ctgggtggag caggggattg gttgttcaaa
     8221 agttccggat attcacaatg tggcgctgat ggaagaccgc gcaacgctgc gtatctccag
     8281 ccagcatatc gccaactggt tacgtcacgg tattctgacc aaagaacagg tgcaggcgtc
     8341 gctggagaat atggcgaaag tggttgatca gcaaaacgct gacgatccgg cttatcgtcc
     8401 gatggcgggg aatttcgcta actcgtgtgc ttttaaagct gccagcgatt taatcttcct
     8461 cggcgtgaaa cagccaaacg gctataccga accgttatta cacgcctggc gtttacgcga
     8521 aaaagaaagt cattaatagc gtcaaaaaaa gccccgacaa gttttattgt cggggctatg
     8581 atagtaactg cggtagtaca tttatcccgg aaataaccgg agcgattatc agggtttaac
     8641 aatattaaat acccgtagcg cattactagc gggcctataa caacaatatg gacatcttaa
     8701 cgatactctg cgcctgcgtg tgcagaacag aaacccttac ccgatatact tgttatgcca
     8761 gaggtagcaa ttgcgttagc ggcgtactgt tgctgtaaag tataacgcct atattttaca
     8821 gttcagtcct cttttgctta ttacaacctt aagtatatca agcatataaa gataataaga
     8881 gactgaacaa tatggttacc tggacccaaa tgtatatgcc gatgggagga ctggggctat
     8941 ccgctctggt cgccctgatc ccgataatat tcttcttcgt tgcactcgcg gtattacgtc
     9001 tgaaaggaca tgtcgctgga gcaataaccc ttatattatc tatcctgatt gcaatattcg
     9061 cctttaaaat gccgattgat atggcttttg ctgctgcggg ctatggcttt atttatggat
     9121 tatggcctat tgcgtggatt attgtcgcgg cggtgttcct ctataaatta accgttgcca
     9181 gcggtcagtt cgatattatc cgcagctcgg ttatctccat caccgacgat cagcgtttgc
     9241 aggtgttact gattggtttc tcctttggtg cgttactgga aggagcggct ggctttggtg
     9301 cgccggtggc gattaccggt gcgctgctgg tgggcctggg cttcaaaccg ttatacgcgg
     9361 cggggctgtg tctgattgcc aacactgcgc cagtggcgtt tggtgcgttg ggcgtgccga
     9421 ttctggtcgc cggtcaggta acgggaatcg atccgttcca cattggcgca atggcgggac
     9481 gtcagttacc gttcctgtcg gttcttgtgc cgttctggct ggtagcaatg atggacggct
     9541 ggaaaggggt gaaagagacg tggccagcgg cgctggttgc tgggggaagc tttgctgtca
     9601 ctcagttctt tacctctaac tatattggtc cggaactgcc ggatattact tcggcgctgg
     9661 tgagtatcgt ctcactcgct ttattcctta aagtctggcg gccgaaaaat accgaaacgg
     9721 caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa taagccatct tctggcggtc
     9781 ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc gtggtcaccg tttttaatct
     9841 taacggtgct ggtcaccatc tggaccatga agccgtttaa agcgttattt gctccgggcg
     9901 gcgcgtttta ttcactggtg attaatttcc agatccctca tttgcatcaa caagtgttga
     9961 aagcggcacc cattgtcgcc caaccaacgc caatggatgc ggtgtttaaa ttcgaccctc
    10021 tctcggctgg cggcaccgct atttttattg cggcgattat ctctatcttc atcctcggcg
    10081 tgggcatcaa gaaagggatt ggcgtctttg ccgaaacgct aattagtctg aagtggccga
    10141 tattgtcgat tggcatggtg ctggcgttcg ccttcgtcac caactattcc ggcatgtcca
    10201 ccacgttggc gctggtactg gcaggtacag gcgtgatgtt cccgtttttc tcaccgttcc
    10261 tcggctggct gggggtattc cttaccggct cggacacttc ttcaaacgcc ctgtttggtt
    10321 cactgcaatc gaccacggca cagcaaatca acgtctctga cactctgctg gtggcagcaa
    10381 ataccagcgg cggcgtaact ggcaagatga tctccccgca atctatcgcc gtggcctgcg
    10441 ccgcgacggg catggtgggc cgagaatctg aactattccg ctacaccgtg aagcacagtc
    10501 tgatttttgc cagcgttatc ggcgttatca cgttgttgca ggcgtatgtg tttactggaa
    10561 tgttggtctc gtaaatcaat aatgccggat gatcgttaat atcatccggc ttcatttaca
    10621 ttattctgtc tta
//
LOCUS       DAPHDX010000143        10441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPHDX010000143 DAPHDX010000000
VERSION     DAPHDX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.145"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            83..658
                     /gene="ssuE"
                     /locus_tag="RH058_003755"
     CDS             83..658
                     /gene="ssuE"
                     /locus_tag="RH058_003755"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV3139281.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            651..1610
                     /gene="ssuA"
                     /locus_tag="RH058_003756"
     CDS             651..1610
                     /gene="ssuA"
                     /locus_tag="RH058_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV3139282.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1607..2752
                     /gene="ssuD"
                     /locus_tag="RH058_003757"
     CDS             1607..2752
                     /gene="ssuD"
                     /locus_tag="RH058_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV3139283.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2763..3554
                     /gene="ssuC"
                     /locus_tag="RH058_003758"
     CDS             2763..3554
                     /gene="ssuC"
                     /locus_tag="RH058_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV3139284.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3551..4318
                     /gene="ssuB"
                     /locus_tag="RH058_003759"
     CDS             3551..4318
                     /gene="ssuB"
                     /locus_tag="RH058_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3139285.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4525..7137)
                     /gene="pepN"
                     /locus_tag="RH058_003760"
     CDS             complement(4525..7137)
                     /gene="pepN"
                     /locus_tag="RH058_003760"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV3139286.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7403..8605
                     /gene="pncB"
                     /locus_tag="RH058_003761"
     CDS             7403..8605
                     /gene="pncB"
                     /locus_tag="RH058_003761"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV3139287.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8774..10174
                     /gene="asnS"
                     /locus_tag="RH058_003762"
     CDS             8774..10174
                     /gene="asnS"
                     /locus_tag="RH058_003762"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV3139288.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVTGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2359 a         2700 c         2808 g         2574 t
ORIGIN      
        1 gcattttctt gtctctccga cagactgacc gaattgttat caatgttaac aaaaaaagaa
       61 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
      121 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tcgatgtaga
      181 ggtttatcac tggaatctgc aaaacttcgc cccggaagat ctgctttatg ctcgtttcga
      241 tagtccggca ctcaagacct tcaccgaaca gctgcaacag gctgatggac tgatagtcgc
      301 tacacctgtg tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc
      361 agagcgcgct ttgcaaggca aagtggtgct accgctggcg acgggcggta ccgtggccca
      421 tctgctggcg gtcgattatg cccttaaacc agttttaagc gcactgaaag ctcaggagat
      481 cctgcacggc gtgtttgccg atgactcaca agtaattgat taccatcaca gaccccagtt
      541 cacgccaaat ctgcaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca
      601 ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaca
      661 tcattaaact ggcgctggtg ggattgctta gcgtctctac gattgcggtt gctgcagaat
      721 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtactggcaa
      781 aaagccacca gttactggaa aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc
      841 ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag tattgatctc ggcagtaccg
      901 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatat gtgggcgtcg
      961 agccaccgaa gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
     1021 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt
     1081 tactgcgtgc actgcgtcag gccggactta agtttaccga tatccaaccc acttacctga
     1141 cgcccgctga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     1201 atccctacta ctctgctgca ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg
     1261 atctcaatca aactggatcg ttttatctgg cagctcgtcc ctatgcagaa aaaaacggcg
     1321 cttttattca gggcgtactc gcaaccttta gtgaggccga tgcgttaacc cgcagccagc
     1381 gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct
     1441 cttacttaga tcatcgccct cctaccacca tcaaaccggt taacgccgag gttgccgcct
     1501 tacagcagca aacggcagat ctgttttatg aaaatcgtct ggtgccgaaa aaagtcgata
     1561 ttcgccagcg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     1621 gttctggttt ttaccgaccc atggtgacgg gcattatctt ggaacggaag aaggttcacg
     1681 cccggttgat cacggctatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac
     1741 cggtgtgctg atcccaacgg ggcgatcgtg tgaagatgcg tggctggtgg cggcgtcgat
     1801 gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgccccagcg taacctcacc
     1861 taccgttgcc gctcgccagg ccgccacgct tgaccgtctc tcaaatggtc gtgcgttgtt
     1921 taatctggtc acaggcagcg atccacaaga gctggcaggc gacggcgtgt tccttgatca
     1981 tagcgagcgc tacgaagctt cggcggaatt tacccaggtc tggcggcgct tattgctggg
     2041 ggaaaccgtc aatttcaacg gtaaacatat tcatgtgcgc ggagcaaaac tgctcttccc
     2101 gccgattcaa cagccttatc ctccgcttta ctttggcgga tcgtcagacg tcgcccagga
     2161 gctggcggca gaacaagttg atctctacct cacctggggc gaaccgccag aactagtaaa
     2221 agagaagatc gaacaagtgc gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg
     2281 tattcgtctg catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg
     2341 gttaatctcg catcttgatg atgaaactat cgccaaagca caggccgcat tcgcccggac
     2401 ggattccgta ggacaacagc gaatggcggc gttacataac ggcaagcgcg acaatctgga
     2461 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg gaacggcgct
     2521 ggtgggcgat ggtcctacgg tcgctgcgcg aatcaacgaa tacgccgcgc ttggcatcga
     2581 cagctttgtg ctttcgggct atccgcatct ggaagaagcg tatcgggtcg gcgagttgct
     2641 gttcccacat ctggatgtcg ccattccgga aattccccag ccgcagccgc tgaatccgca
     2701 aggcgaagcg gtggcgaatg attttatccc ccgtaaagtc gcgcaaagct aaggaagaaa
     2761 taatggcaac gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg
     2821 gcatcgtggc ggtgtggcaa ctggcctcct cagttggctg gctgtcgacg cgtattttgc
     2881 cctcaccgga aggggtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
     2941 agcatctggc gatcagttcc tggcgggcgc tgattggctt ttcaattggc ggatcgctgg
     3001 ggttgatttt gggactgatt agcgggctat ctcgctgggg agaacggctg ctggatacct
     3061 caattcagat gttgcgcaac gtaccgcatc tggcgctgat cccgctggtg attttgtggt
     3121 ttggcattga tgaatccgca aaaatctttc tggtggcgct cggcacgcta ttccccattt
     3181 atatcaacac ctggcatggc atccgtaata tcgatcgcgg tctggtggaa atggcgcgta
     3241 gctatggatt atccggcata ccgctgttta tccatgtgat cctgcctggt gccctgccct
     3301 caattatggt cggcgtgcgt tttgcgttgg gcctgatgtg gctgacgctg attgttgccg
     3361 aaaccatttc tgccaattca ggcattggtt atctggcgat gaatgcgcgg gagtttttgc
     3421 aaacggacgt ggtggtagtc gctattattc tttacgccct gctcggcaaa cttgctgacg
     3481 tcagcgcgca gctgttagag cgcctctggt tgcgctggaa cccggcttat catttgaagg
     3541 aggccacggt atgaatactg ctcgtctgaa ccagggcaca ccattgttgc tcaatgcagt
     3601 aagcaaacat tacgcggaaa atatcgtcct gaaccaactg gatttacata ttccggcagg
     3661 tcagtttgtg gcggtggtgg gccgcagcgg tggtggcaaa agtaccctgc tgcgcctgct
     3721 ggccgggctg gaaacgccaa ccgcaggcga tgtgttggca ggcaccacac cgctggctga
     3781 aattcaggaa gacacgcgaa tgatgtttca ggatgcgcgt ctgctaccgt ggaaatcggt
     3841 gattgataac gttgggttag gccttaaagg ccagtggcgc gatgccgcgc gtcgggcgtt
     3901 ggctgcggta ggactggaga atcgcgccgg ggaatggcct gccgcacttt caggcgggca
     3961 gaaacagcga gtggcgctgg caagagcgtt gattcatcga ccgggtttat tgttgcttga
     4021 tgaaccgctc ggggcactgg acgccttaac gcgactcgag atgcaggatt tgattgtgtc
     4081 actttggcag gagcacggct ttaccgtact gttggtgacg catgatgtga gcgaagcggt
     4141 agcaatggct gaccgggtgt tgttaattga agagggaaaa attggtctgg atttgacagt
     4201 ggatatcccc cgtccgcgtc gattagggtc ggtgcgatta gcggagctgg aagcggaagt
     4261 attgcaacgg gtaatgcggc gtgggcattc tgaacaactg attcggcgtc atggttaata
     4321 tgccggatgt ggcgcacaaa accagcattt tcaacggact agtaggcctg ataagacacg
     4381 tcaagcatcg catcaggcag ttgggcacgg ctgccggatg cggcgtgaac gccttatcca
     4441 gcctacaaaa ccagcaattt caacggagtt gtaggcctga taagacgcgt caagcgtcgc
     4501 atcaggcatt ttggctcaaa agtatcaagc cagtgctttg gtgatcttct cgtacagatc
     4561 gccagagaga ttttccagcc ctttcaactg ttccagcgcc gcgcgcattt tctcctgacg
     4621 tttggcatca taacgtttca ggcgaatcag cggttcaatc agacgtgaag ctacctgcgg
     4681 gttacggctg ttgaggtcgg taagcatttc caccaggaac tggtaaccgc tgccatcttc
     4741 ggcatggaac gctgccgggt tgctgcccgc aaacgcacca atcaacgaac gaatacggtt
     4801 cgggttgctc atggtaaatg agcgatgctg caacagacca cgcaccgtct ccagcacatt
     4861 cgccgccggg ctggtggctt gcaggataaa ccatttatcc atcaccagac cgtcctgatg
     4921 ccacttatcg tcgtactcct gcatcagcgc gtcacggcaa ggcagctgtg cagcaaccgc
     4981 cgcagaaagc gctgccagcg catcggtcat attgtttgct tcatggtatt gcttgctcac
     5041 cagcacgtca gccagatgcg tttcaccaaa agcaaggaag cgcaggcagg cattacgcag
     5101 agtgcgtttt gcaatatctt catgctcaac acggtactcg ctctggtaat tcgcgttgta
     5161 aatagccagc agctcatccg ccagttcagt cgccagagta cgagtgagtg cttcgcgtac
     5221 ttcggcaata gcaatcgggt cgatgatatc gaacagttca gccatttcat tgacagaagg
     5281 cagcgtcagg atttctgccg ccagcgccgg atcaatcttc tcatcgagca gcaccgcgcg
     5341 gaaagcgtca gccacatgta ccggcagaga cagcggctgc ccttgctgat gacgggcgac
     5401 gttcagcttg atgtaggttg ccagcaaact ttgcgccgca tcccaacggg agaaatcatt
     5461 acgcgcatga cgcatcagga aggtcagttg ctgatcgctc cacttatatt ccagtttcac
     5521 tggcgcagag aattcgcaca gcagcgcagg caccggctgg aagtagacat tatcaaaaac
     5581 aaaggtctgt tccgcctggg tgacgttcag cacggaattc accggatgac cgcctttctg
     5641 caacgggatc actttgcctt cgttgtcata caattcgatg gcaaatggaa tatgcagcgg
     5701 ctgtttttct gcctgatccg gcgtggctgg cgtgcgctgg ctgatggtca gggtgtactg
     5761 ctcggtttcc ggattgtagt cgtctttgac ggtcacaatc ggcgtgccgg actggctgta
     5821 ccaacggcgg aaatgggaga gatcgacatt cgacgcatct tccatcgcct gcacaaagtc
     5881 gtcgcaggtt gccgcgctgc catcatggcg ctcgaaatag agttgcatcc ctttctggaa
     5941 gttttcttcg cccagcaggg tgtggatcat gcgaatcact tccgcgccct tctcgtaaac
     6001 ggtcagggtg tagaagttgt tcatctcaat gaccatatcc gggcggatcg ggtgcgccat
     6061 cgggctggcg tcttccgcaa actgcaatcc gcgcatggtg cgcacattat tgatacggtt
     6121 taccgcgcgg gaaccaaggt cagagctgaa ttcctgatcg cggaagacgg ttaaaccttc
     6181 tttcaggctg agctggaacc agtcgcggca ggtcactcgg ttaccggtcc agttatggaa
     6241 atattcatgg ccgataacgc gttcaatatc gagataatct ttgtcggtgg cggtgtcggt
     6301 gcgggccaac acatatttgg agttaaagat attcagaccc ttattctcca ttgcgcccat
     6361 attgaagaaa tccaccgcga cgatcatata gatgtcgagg tcatactcca ggccaaagcg
     6421 ttcttcatcc catttcatgg agtttttcag cgaggtcatc gcccacggcg cgcgatcaag
     6481 gttgccgcga tcgacgtaca gctccagtgc tacttcgcga ccagaacgcg tggtaaaggt
     6541 atcgcgcagt acatcaaagt cgcctgccac cagcgcaaac aggtagcacg gtttcgggaa
     6601 cgggtcctgc cactgtaccc aatggcgtcc gttttcgagt tcgccttgcg caacgcggtt
     6661 accattggaa agcaggaagg gatatttgat tttatcggca ataattttgg tggtaaaacg
     6721 cgccagcacg tccgggcggt cgagataata cgtaatatgg cggaaacctt cggcttcaca
     6781 ctgggtgcaa agcgcatcgc ctgactgata aagcccttcc agcgccgtat tcgccgccgg
     6841 gcttatttca ttaatgatct taagcgtaaa acgctccggc aaattactga tgaccagtgc
     6901 gccctcttct tctttccagg cggtccacgg ctcatcatta atatgaacag aaaccagttt
     6961 gaggtcttcg ccgttgagac gaagcggagc atctgatgca ccatgacgga cagcctggct
     7021 gaccgcggta acgaccgtct tttgcgcgtc gaggtcaaag gtcaagtcaa tatcagtaat
     7081 ctggtaatcc ggcgcacgat aatcgtgacg gtatttggct tgtggctgtt gagtcataaa
     7141 taacctttag catcttttat agagtctggt gttcagtcta ttcctgttgc gtaaatcgcg
     7201 ctatgcagaa tcttcatctt ttcaggtaca aacgccttta ttgctacatt tttataacat
     7261 acaccgcgta atgccatcga ccagaaaggt ggcatatggt gtgatcgggg ttcaataaat
     7321 tgcgaaacaa ggtatactcc agcagttcct gaagatgttt attgtactaa acgctcctgt
     7381 acgaggacgc tactgcgcac ctatgacaca attcgcttct cctgttctgc actcgttgct
     7441 ggatacagat gcttataagt tgcatatgca gcaagccgtg tttcatcact attacgatgt
     7501 gcatgtcgcg gcggagtttc gttgccgagg tgacgatctg ctgggtattt atgccgatgc
     7561 tattcgtgaa cagattcagg cgatgcagca cctgcgcctg caggatgatg aatatcagtg
     7621 gctctctgct ctgcctttct ttaaggccga ctatcttaac tggttacgcg agttccgctt
     7681 taacccggaa caagtcaccg tatccaacga taatggcaag ctggatattc gtttaagcgg
     7741 cccgtggcgt gaagtcatcc tctgggaagt tcctttgctg gcggttatca gtgaaatggt
     7801 acatcgctat cgctcaccgc aggccgacgt tgcgcaagcc ctcgacacgc tggaaagcaa
     7861 attagtcgac ttctcggcgt taaccgccgg ccttgatatg tcgcgcttcc atcttatgga
     7921 ttttggcacc cgtcgccgtt tttctcgcga agtacaagaa accatcgtta agcgtctgca
     7981 acaggaatcc tggttcgtgg gcaccagcaa ctacgatctg gcgcgtcggc tttccctcac
     8041 gccgatggga acacaggcac acgaatggtt ccaggcacat cagcaaatca gcccggatct
     8101 agccaacagc cagcgagctg cacttgctgc ctggctggaa gaatatcccg accaacttgg
     8161 cattgcatta accgactgca tcactatgga tgctttcctg cgtgatttcg gtgtcgagtt
     8221 cgccagtcgc tatcagggcc tgcgtcatga ctctggcgac ccggttgaat ggggtgaaaa
     8281 agccattgca cattatgaaa agctgggaat tgatccacag agtaaaactc tggttttctc
     8341 tgacaatctg gatttacgca aagcggttga gctataccgc cacttctctt cccgcgtgca
     8401 attaagtttt ggtattggga ctcgcctgac ctgcgatatc ccccaggtaa aacccctgaa
     8461 tattgtgatt aagttggttg agtgtaacgg taaaccggtg gcgaaacttt ctgacagccc
     8521 tggcaaaact atctgccacg ataaagcgtt tgttcgggcg ctgcgcaaag cgttcgacct
     8581 tccgcatatt aaaaaagcca gttaatatca tcagggagct aatcggctcc ctttttttac
     8641 ctttaattcc gaaatctttc gctgcatttg cgaattctgc ttgtctgatt gcagatgcca
     8701 ggtaacatag gtatcccccc atttacgggt ggacaagtgt ttattttttc cgactattaa
     8761 cagagagaat attatgagcg ttgtgcctgt agccgacgta ctccagggcc gcgtagccgt
     8821 tgacagcgaa gtcaccgtac gcggatgggt acgtacccgc cgagattcaa aagctggcat
     8881 ctccttcctc gccgtttatg acggttcctg ctttgatcct gtacaggctg tcatcaataa
     8941 ttctctgccc aattacaatg aagacgtcct gcgtctgacc accggctgct cggtcattgt
     9001 gacgggtaaa gtcgtggcgt cgccgggcca ggggcaacaa tttgaaattc aggccagcaa
     9061 ggttgaagtt actggttggg ttgaagatcc agacacttac ccgatggcgg caaaacgcca
     9121 cagcattgag tatctgcgtg aagtcgctca cctgcgtccg cgcacaaacc tgattggtgc
     9181 cgtcgcgcgc gttcgccata cgctggcgca ggcgctgcat cgcttcttta acgagcaggg
     9241 attcttctgg gtttcaacgc cactgattac cgcatctgat accgaaggtg caggcgaaat
     9301 gttccgcgtt tctacgctgg atctggaaaa cctgccgcgt aacgatcagg gcaaagtgga
     9361 tttcgacaaa gacttctttg gtaaagagtc tttcctgacc gtatctggcc agttgaacgg
     9421 cgaaacctac gcttgcgcat tatccaaaat ttataccttc ggcccgactt tccgtgctga
     9481 aaactccaac accagccgtc acctggcgga attctggatg ctggagccgg aagtggcgtt
     9541 tgctaacctg aacgatatcg cgggtctggc tgaagccatg ctgaaatatg tcttcaaagc
     9601 ggttctcgaa gaacgcgcag acgacatgaa attcttcgct gaacgcgtag ataaagatgc
     9661 cgtttcacgt ctggaacgct tcattgaggc cgatttcgcg caggtggatt acaccgacgc
     9721 agtgaccatt ctcgaaaact gcggcaggaa gtttgaaaac ccggtttact ggggcgtcga
     9781 tctctcttct gagcatgagc gttatctggc ggaagaacac tttaaagcac cggtagtggt
     9841 taaaaactat ccgaaagata ttaaagcgtt ctatatgcgc ctcaacgaag acggtaaaac
     9901 cgttgcggct atggacgttc tggctccggg catcggtgag atcattggtg gctcccagcg
     9961 tgaagaacgt ctggacgttc tggacgagcg tatgctggaa atgggcctga ataaagaaga
    10021 ttactggtgg tatcgcgatc tgcgtcgcta cggtactgtt ccgcattcag gtttcggtct
    10081 tggttttgaa cgcctgattg cttacgtaac tggcgtgcaa aacgtgcgtg atgtgattcc
    10141 gttcccacgt actccgcgca acgccagctt ctgattttct tcctcaaaga gccagcgcct
    10201 gctggctctt ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag caatctattt
    10261 gcaaccccgc cataacgatc atcctgttac ggaatattac attgcaacat ttacgcacaa
    10321 aaaataaccc gcattcttat tgcggattag ttttttctta gctgatagca caattttcat
    10381 actatttttt ggcattctgg atgtctgaaa gaggattttg tgccaggtcg ataaagtttc
    10441 c
//
LOCUS       DAPHDX010000144        10312 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPHDX010000144 DAPHDX010000000
VERSION     DAPHDX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.146"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..629
                     /locus_tag="RH058_003763"
     CDS             87..629
                     /locus_tag="RH058_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139289.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            729..1931
                     /locus_tag="RH058_003764"
     CDS             729..1931
                     /locus_tag="RH058_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV3139290.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            1986..2456
                     /locus_tag="RH058_003765"
     CDS             1986..2456
                     /locus_tag="RH058_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3139291.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2497..3399)
                     /locus_tag="RH058_003766"
     CDS             complement(2497..3399)
                     /locus_tag="RH058_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV3139292.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSPNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3593..4783)
                     /gene="glpC"
                     /locus_tag="RH058_003767"
     CDS             complement(3593..4783)
                     /gene="glpC"
                     /locus_tag="RH058_003767"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV3139293.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4780..6039)
                     /gene="glpB"
                     /locus_tag="RH058_003768"
     CDS             complement(4780..6039)
                     /gene="glpB"
                     /locus_tag="RH058_003768"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV3139294.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6029..7657)
                     /gene="glpA"
                     /locus_tag="RH058_003769"
     CDS             complement(6029..7657)
                     /gene="glpA"
                     /locus_tag="RH058_003769"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV3139295.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7930..9288
                     /gene="glpT"
                     /locus_tag="RH058_003770"
     CDS             7930..9288
                     /gene="glpT"
                     /locus_tag="RH058_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV3139296.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9293..>10312
                     /gene="glpQ"
                     /locus_tag="RH058_003771"
     CDS             9293..>10312
                     /gene="glpQ"
                     /locus_tag="RH058_003771"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV3139297.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGV"
BASE COUNT         2344 a         2582 c         2862 g         2524 t
ORIGIN      
        1 tttttcgctc attgaatcct ctgtgccagg cagtacaatc ccgcagcttt gggaattttg
       61 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
      121 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
      181 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact
      241 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
      301 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
      361 atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
      421 gtttgtttgg cgagtattac tactctccgg attcgctctc cagcggtatt caaagttatg
      481 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
      541 atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga caacgccgtg gctgacggcc
      601 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
      661 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
      721 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
      781 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ctgccattat
      841 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
      901 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa
      961 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca
     1021 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     1081 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     1141 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     1201 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     1261 cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     1321 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcttca atgccgatta
     1381 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     1441 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     1501 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     1561 gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg
     1621 ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca
     1681 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgaacat ctcaactttg
     1741 cgctagccac accagacggc actttcgctc tgcgtgtgcg tttcagcact acgcgctaca
     1801 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt
     1861 taaacggcca ggatatcgcc agtgagcatg gctggattga ggttattgag tccatgacct
     1921 tatctgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     1981 aaaacttgtc caaccctccc tacggtgagc gtggtgttgg ggccagtgtg gcacgggctg
     2041 cgcgctgggg acgcattgag aattacatgg cgcaagtcaa cgattcgctt tgtctgttag
     2101 tgcaggtgga aagtaaaacg gcactggata acctggacga gatcctcgac gtcgaaggga
     2161 ttgatggcgt gtttattgga ccagcggatc tttctgcgtc gttgggctac ccggataacg
     2221 ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc cgtgctgcgg
     2281 gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc ctggcgtggg
     2341 gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc ctggatcaac
     2401 gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtagt tattgatatc
     2461 aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg ctgccagatc
     2521 ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga tcaatgccct gttccagcat
     2581 gcgcaaagca attttaatgg cttgttcatg cataccttcc tgccagccaa tttgatgccc
     2641 ttcctgccgt agccgttcag caatagtcat taatctctcc ttttgtagtg gtgaacgttc
     2701 ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg ccgcattgca gcagataatt
     2761 aaacagtgtt tgtagctggc tgtcattagt gaagcctcta accaaaagcg tggtgatcct
     2821 gtcgaccatg ccgattaaat cgcggtcgcg aatatgcttt tgaatcagtt ccagcagagc
     2881 tatacgccga tgttgcatga tctcatcgtc aggtacgatg gtgatatcca ccaacggaaa
     2941 agcttcggtg tacaactgcc gggcaagttg cggatcgtca aactcatcca gccagttagg
     3001 tgagtacggg tagggcgatg tttcgccatg ataaaacagc aatggcacca ccagcggaac
     3061 tctgtcatag cctttatcca ggtgacgctg catggcggca gtggcatagc gcattagccg
     3121 aaaagccata ttcttttctg cagagctttg atgttcaatc acgcagtaga tatagccgtc
     3181 accgtcgctg gtttccacgg accacaaaac atccgagtag taagcgcgta aacttttttc
     3241 aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg caaagcttgc gcagtggttc
     3301 tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt gtgaacataa aggttttaaa
     3361 caccgcatca tgcggggagg atgttgttga ttcggtcatg gttgtccgtc acctgatatt
     3421 tatgatgacg cgacattacg tctatcccgg gcctttgaca tctgtcagtt tcagcctttg
     3481 cgagacattt tcagaggaat tttcaaagcg ttgcaacgca tacatggtgc gtggaatcgc
     3541 gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca tcaaaaggtc ggttaagcca
     3601 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag gctcgtggac atctcaatct
     3661 gccatttgca ggtttcgcag tcggtgatga ccagatccgc gccgctctct tctatctggc
     3721 ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     3781 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggattttac
     3841 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3901 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3961 acagccagcg ggttgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     4021 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     4081 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     4141 ctttatcggt aaagccgttg gcgattagcg gtacgccgca gcatttttct ttgctgagca
     4201 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     4261 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatat tgtgcttgct
     4321 gagccgctat gctgcgatac cagcgacgga acgtgccgaa ggagtatttc ggtagcgtgc
     4381 ggcgatgatc gatttttaac gccgcatcaa gcagctgccg caccggtttc agcgaggtag
     4441 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatgactca
     4501 acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt tgcccgcgcg cgctggataa
     4561 tatcgccaat cttcacatcg gacgggcagg cgacttcaca acgtttgcag ttgatgcaat
     4621 atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg cagacgctcg ccatccggcc
     4681 cggcttgttt tggccctgga taaccgggat tcacccggct caccgggcag gcggtggtgc
     4741 agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt cattgttggc ctcctgcgcg
     4801 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4861 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt
     4921 ttgccctgcc tgcgacgggc gtagcgtctc atcagtggtt acaccgaact gctgccacgg
     4981 ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     5041 aaggccgaga atcggctcgc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     5101 actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga atatcggcgt gattgcgggt
     5161 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     5221 ccacacgcca ccctggcgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     5281 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     5341 acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     5401 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     5461 acgggcgata ttcacagcgc gaaattcggt ggcgttattg cgcaggacat ccagttccgg
     5521 cagctctatt tctgcggttt caaccgaaag atccagttcg cgcaacgaag ccgccgcaag
     5581 atgcgcctgg aaatccatca gcccgcttat ccccactaca catatcttct tcgcgggtag
     5641 cggccagacg ggaacttctg gcgaacttaa ccaggttgca cgcaaagtgc cgagcggggt
     5701 aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc gcaccgcttt cagcgatcaa
     5761 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggt tcgagaagac tgtaaggatg
     5821 ggctggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca ccggttgacc
     5881 atcaggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5941 acgcgtgaca atggcacagc gcaggccgtg tttttgcagt tgcaggccac agagtaatcc
     6001 ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc tttctgctcc ttctccagac
     6061 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgcg tctccccagg
     6121 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     6181 gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc agcgcggcag gcgcagagtt
     6241 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     6301 cgtttaaatt ttctaccgcg tactgaactt cacccgcagt taccgcttcg cactcacata
     6361 ctaggctacg atgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     6421 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     6481 cgggttcttg tgaaccaggc agtgccagat cggcagtcgt acaggggcgc gtgttgccca
     6541 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     6601 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     6661 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     6721 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     6781 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6841 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6901 cggaaggttt acggcagcgg ttgatcacat gctggttaat gcggtgatcc atgatcagca
     6961 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     7021 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     7081 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     7141 catgagcggt aaggataacg gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     7201 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     7261 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     7321 cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc atcttccggc agggtgataa
     7381 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     7441 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     7501 gaccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     7561 gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc cgttgcgccg ccaccaatga
     7621 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     7681 tttaccattt agccatagta aaaacatgaa ttgtttgatt tcgcgcatat tcgctcataa
     7741 ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat tttgtaaatc ttatttaata
     7801 atgtgtgcgg caattcacat ttaatttctg aatgttttct taacatcgcg gcaactaaag
     7861 aaacggcagg ttctcccact gaatcagtct gttaatcata aataagacca cgggccacgg
     7921 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgctgcg
     7981 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     8041 tatgcggctt actatttggt tcgtaagaac tttgcgcttg ctatgcctta tctggtcgag
     8101 cagggattct ctcgcggtga tttaggtttc gcgctttcgg ggatctcgat cgcttatgga
     8161 ttttcgaaat tcataatggg ttcggtatca gatcgctcga atccgcgcgt tttcctcccc
     8221 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     8281 tcgagcattg cggtgatgtt tgtacttttg ttcctctgcg gttggttcca ggggatgggg
     8341 tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     8401 gtgtcagtgt ggaactgtgc gcacaacgtt ggtggtggta ttccgccgct gctgttcctg
     8461 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     8521 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     8581 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     8641 gagctgacgg cgaagcaaat cttcatgcag tacgtactgc cgaacaaact gctgtggtat
     8701 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     8761 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcctt
     8821 tatgaatatg caggtattcc gggcactctg ctgtgcggct ggatgtcgga taaagtcttc
     8881 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8941 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     9001 ggcttcctga tctacggtcc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     9061 aaaaaagcgg caggtacggc agcgggcttt accgggctgt ttggttacct gggcggttcg
     9121 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     9181 atggtaatga ttggcggcag cattctggcg gttatcttgt tgattgttgt gatgattggc
     9241 gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     9301 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     9361 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     9421 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     9481 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attatctcga
     9541 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     9601 cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa tttaccgaag gtttcgatat
     9661 tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc ccaatgggta agtccgactt
     9721 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     9781 gaaaaatatc ggtatttatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9841 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9901 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9961 acccaaaatg ggcatggagc tcaatctggt acagctgatt gcctataccg actggaatga
    10021 aacgcagcag aaacagccgg atggaagctg ggttaattac aactacgact ggatgtttaa
    10081 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
    10141 gttgattgag gaaacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
    10201 tcagcagaat aaactggtag tgcatcctta taccgtgcgg tcggataaac tgcctgaata
    10261 cactactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg ta
//
LOCUS       DAPHDX010000145        10163 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPHDX010000145 DAPHDX010000000
VERSION     DAPHDX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.147"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            111..1085
                     /locus_tag="RH058_003772"
     CDS             111..1085
                     /locus_tag="RH058_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3139298.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            2063..2533
                     /gene="accB"
                     /locus_tag="RH058_003773"
                     /gene_synonym="fabE"
     CDS             2063..2533
                     /gene="accB"
                     /locus_tag="RH058_003773"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV3139299.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            2544..3893
                     /gene="accC"
                     /locus_tag="RH058_003774"
     CDS             2544..3893
                     /gene="accC"
                     /locus_tag="RH058_003774"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV3139300.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            4002..4244
                     /locus_tag="RH058_003775"
     CDS             4002..4244
                     /locus_tag="RH058_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV3139301.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            4234..5685
                     /gene="panF"
                     /locus_tag="RH058_003776"
     CDS             4234..5685
                     /gene="panF"
                     /locus_tag="RH058_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV3139302.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            5697..6578
                     /gene="prmA"
                     /locus_tag="RH058_003777"
     CDS             5697..6578
                     /gene="prmA"
                     /locus_tag="RH058_003777"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV3139303.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6907..7872
                     /gene="dusB"
                     /locus_tag="RH058_003778"
     CDS             6907..7872
                     /gene="dusB"
                     /locus_tag="RH058_003778"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV3139304.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGEMLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7898..8194
                     /gene="fis"
                     /locus_tag="RH058_003779"
     CDS             7898..8194
                     /gene="fis"
                     /locus_tag="RH058_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV3139305.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            8280..9164
                     /gene="yhdJ"
                     /locus_tag="RH058_003780"
     CDS             8280..9164
                     /gene="yhdJ"
                     /locus_tag="RH058_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV3139306.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            9248..9427
                     /locus_tag="RH058_003781"
     CDS             9248..9427
                     /locus_tag="RH058_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV3139307.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(9430..10092)
                     /gene="acrS"
                     /locus_tag="RH058_003782"
     CDS             complement(9430..10092)
                     /gene="acrS"
                     /locus_tag="RH058_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV3139308.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2498 a         2468 c         2628 g         2569 t
ORIGIN      
        1 aaatcagatt tatctcgcaa tttattgcgc ttcatcggct ttgcttttcc attagcgagt
       61 atagtcttca gaaattattt tccaatccat catgcacatg aggaccactt atgcaggcgt
      121 tacttttaga acagcaggac ggcaaaactc tcgcatcggt acagacactt gacgaaagtc
      181 gcctgccgga gggcgatgtc acggttgatg ttcactggtc gagcctgaac tataaagatg
      241 cgctggcgat taccggtaag ggaaaaatca tccgtaattt tccgatgatt cctgggatcg
      301 attttgccgg aactgtacgc accagcgaag atccgcgttt tcatgccggt caggaggtgt
      361 tactcactgg ctggggcgtt ggtgaaaacc actggggtgg gctggcggag caggcgcgag
      421 tgaaaggtga ctggctggtt gccatgccgc aagggctgga cgcgcgtaaa gcaatgatta
      481 tcggtactgc cggttttacc gccatgctgt gtgtgatggc gctggaagat gccggtgttc
      541 gcccgcagga cggggagatt gtcgtgacgg gtgccagtgg tggcgtcggc agtaccgccg
      601 tggcgctgct gcataagttg ggttatcagg tcgttgccgt ttccggtcgc gaaagtaccc
      661 atgaatatct gaaaagttta ggtgccagtc gtattctccc tcgtgatgag tttgccgaat
      721 cccgtcctct ggaaaaacaa gtctgggctg gggcaattga cactgttggc gacaaagtgc
      781 tggcaaaagt gctggcgcaa atgaattacg gcggctgcgt ggcggcctgt ggtctggcgg
      841 gtggttttac tctgccaacc acggtcatgc catttattct gcgtaatgtc cgtttgcaag
      901 gggtggattc agtaatgacg ccaccagaac gccgcgcaca agcctggcag cgactggtcg
      961 ccgatttacc ggaatcattc tatacccagg cggcaaaaga gatatctctg tcagaggcac
     1021 cgaactttgc cgaggccatc attaataacc agatccaggg tcgcacgctg gtgaaggtta
     1081 actaaccatt tagcaaggaa taataagaga gggaactcat tttgacatca ttattcaatc
     1141 ggttaacggg aaaagcggtt agccggacag ctttcgtcga acaccttggt caggaagttg
     1201 tacaacatca tccaaactgg aaagtcatga tttcgactga ccacaaattg atgcgcattg
     1261 atactcctct aaacagccat tattgatatg cctccgtcgc ctgttaggtt tatggtgctt
     1321 tgcctgggcg acactacgct taacctgtta ctgatttctg gtcccatggg gtgaataatc
     1381 tgattttatt tgactacaaa ttaaccactc gaacctattt aatgctgaga attgtcagtc
     1441 ggttgatttt gcgtgcttta gcattcacat ctatccagac gatgcagtga aaattgggta
     1501 atccccagca accgctgcgt aatgtcgtct atcttgtcgc gatcctggca tccctacatt
     1561 atttgtggtc tgtgaagatt atctcattgc agcccctcat cttcgcaggg ctggcttttc
     1621 agcttttcac cttacgttat aagaagttcc gtcgatgatg gcgctaattt cgtgaattgt
     1681 gcggcttgtt gcaaattaca cggtgttgaa ggttatttac atgttagctg ttgattatct
     1741 tccctgataa gaccagtatt tagctgccaa ttgctacgaa atcgttataa tgtgcgacct
     1801 cgtcctccct gacgcagttt ttgcgctgcg gaaaaggtga cattggcgca acgaaggtat
     1861 attttgtttt ttgccggagg atagcagcag atcgctgcac aatgtccgtc aagtctaaca
     1921 ttgacactct ggggcaaaat agaccggcgt cccggcctgc tggaatttat cgctatgcat
     1981 acagctgtcg gggcatacgc tttacagacg gcggtgaaac gcctgtcaca atcacactaa
     2041 acaaagagta cggaacccac tcatggatat tcgtaagatt aaaaaactga tcgagctggt
     2101 tgaagaatca ggcatctccg aactggaaat ctccgaaggc gaagagtcag tacgcatcag
     2161 ccgtgcagct cctgccgcaa gtttccctgt gatgcaacaa gcttacgctg caccaatgat
     2221 gcagcagcca gctcaatcta acgcagccgc tccggcgacc gttccttcca tggaagcgcc
     2281 agcagcagcg gaaatcagtg gtcacatcgt acgctccccg atggttggta ctttctaccg
     2341 caccccaagc ccggacgcaa aagcgttcat cgaagtgggt cagaaagtca acgtgggcga
     2401 taccctgtgc atcgttgaag ccatgaaaat gatgaaccag atcgaagcgg acaaatccgg
     2461 taccgtgaaa gcaattctgg tcgaaagtgg acaaccggta gaatttgacg agccgctggt
     2521 cgtcatcgag taacgaggcg aacatgctgg ataaaattgt tattgccaac cgcggcgaga
     2581 ttgcattgcg tattcttcgt gcctgtaaag aactgggcat caagactgtc gctgtgcact
     2641 ccagcgcgga tcgcgatcta aaacacgtat tactggcaga tgaaacggtc tgtattggcc
     2701 ctgctccgtc agtaaaaagt tatctgaaca tcccggcaat cataagcgcc gctgaaatca
     2761 ccggcgcagt agcaatccat ccgggttacg gcttcctctc cgagaacgcc aactttgccg
     2821 agcaggttga acgctccggc tttatcttca ttggcccgaa agcagacact attcgcctga
     2881 tgggtgacaa agtgtcggca atcgcggcga tgaaaaaagc gggcgttcct tgcgtaccag
     2941 gttctgacgg cccgctgggc gacgatatgg ataaaaaccg tgccattgct aaacgcatag
     3001 gttatccggt gattatcaaa gcctccggcg gcggcggcgg tcgtggtatg cgtgtagtgc
     3061 gcggcgacgc tgaactggca caatccatct ccatgacccg tgcggaagcg aaagccgcat
     3121 tcaataacga tatggtttac atggagaaat acctggaaaa tcctcgccac gtcgagattc
     3181 aggtactggc tgacggtcag ggcaacgcta tctatctggc ggaacgtgac tgctctatgc
     3241 agcgtcgtca ccagaaagtg gtcgaagaag caccagcacc gggaattaca ccggaactgc
     3301 gtcgctacat cggcgaacgt tgtgctaaag cgtgtgttga tatcggctat cgcggtgcag
     3361 gtactttcga gttcctgttc gaaaacggcg agttctattt catcgaaatg aacacccgta
     3421 ttcaggtaga acacccggtt acagaaatga tcaccggcgt tgacctgatc aaagaacagc
     3481 tgcgtatcgc tgccggtcaa ccgctgtcga tcaagcaaga agaagttcac gttcgcggcc
     3541 atgcggtgga atgtcgtatc aacgccgaag atccgaacac cttcctgcca agcccaggca
     3601 aaatcacccg tttccacgca cctggcggtt ttggagtgcg ttgggagtct catatctacg
     3661 cgggctacac cgtaccgccg tactatgact caatgatcgg taagctgatt tgctacggtg
     3721 aaaaccgtga cgtggcgatt gctcgcatga agaatgcgct gcaggagctg atcatcgacg
     3781 gtatcaaaac caacgttgat ctgcagatcc gcatcatgaa tgacgagaac ttccagcatg
     3841 gtggcactaa catccactat ctggagaaaa aactcggtct tcaggaaaaa taagactgca
     3901 aaagcgtcaa aaggccggat tttccggcct tttttattac tggggatcga caacccccat
     3961 aaggtacaat cctcgctttc ttcacccatc agggacaaaa aatggacact cgttttgttc
     4021 aggcccataa agaggcgcgc tgggcgctgg ggctgaccct tttgtatctg gcagtttggt
     4081 tagtagccgc ttacttacct ggcgttgccc ccggttttac cggctttccg cgctggtttg
     4141 agatggcctg catcctgacg ccgctactgt ttattggact gtgctgggcg atggtgaaat
     4201 ttatctatcg cgatatccca ctggaggatg acgatgcagc ttgaagtaat tctaccgctg
     4261 gtcgcctatc tggtggtggt gttcggtatc tctgtttatg cgatgcgtaa acggagcacc
     4321 ggcaccttcc ttaatgagta tttcctcggc agccgctcta tgggcggtat tgtgctggcg
     4381 atgacgctca ccgcgaccta tatcagtgcc agttcgttta tcggcgggcc aggagctgct
     4441 tataaatacg gcctgggctg ggtattgctg gcgatgattc agcttcctgc agtctggctt
     4501 tcactcggta ttctcggcaa gaagtttgcg attcttgcgc gccgctacaa tgcagtgacg
     4561 ctgaacgata tgctgtttgc ccgctaccag agtcgtcttc tggtgtggct ggcgagtttg
     4621 agtttgctgg ttgcgttcgt tggtgcgatg accgtgcagt ttatcggcgg tgcgcgcctg
     4681 ctggaaaccg cggcgggtat tccttatgaa accgggctgc tgatttttgg tatcagcatt
     4741 gcgttatata ccgcctttgg tggctttcgc gccagcgtgc tgaacgacac catgcaaggg
     4801 cttgtgatgc tgattggcac cgttgtgctg cttattggcg tggtacatgc cgctggcggc
     4861 ttaagcaacg cagtacagac cttgcaaacc atcgatccgc aactggttac gccacaaggc
     4921 gctgacgata ttctgtcgcc tgcctttatg acgtcgttct gggtactggt gtgctttggc
     4981 gtgattggcc tgccgcatac tgcggtgcgc tgtatctctt ataaagacag caaagcagta
     5041 caccggggga tcatcatcgg tacgattgta gtcgcaatcc tgatgttcgg tatgcacctg
     5101 gccggagcgt taggtcgtgc agtgatcccc gatctcaccg taccggatct ggtgatcccg
     5161 acgttaatgg taaaagtgct gccacctttt gctgccggga tctttctggc tgcacctatg
     5221 gctgcgatca tgtcgacaat taacgcccaa ctgctgcaaa gttccgctac gatcattaaa
     5281 gatctctatc tgaatatccg tccggatcaa atgcaaaacg agacgcgtct gaagcggatg
     5341 tcggcggtaa ttacgttagt tctcggcgcg ttgctgctgc ttgccgcctg gaagccgcca
     5401 gaaatgatca tctggctgaa tttgctagcc ttcggtgggc tggaagccgt tttcctgtgg
     5461 ccgctggtgc tgggtcttta ctgggaacgc gccaacgcca aaggcgcgct aagtgcgatg
     5521 atcgttggcg gcgtgctgta tgccgtactc gcgacgctga atattcagta cctgggcttc
     5581 caccctattg tgccctcgtt actactaagt ttgctggctt tcctggtcgg aaaccgtttc
     5641 ggtacatccg tcccgcaagc taccgttttg actactgata aataaagagt tttgccatgc
     5701 cttggatcca actgaaactg aacaccaccg gcgcgaacgc ggaagatctt agcgatgcgc
     5761 tgatggaagc gggtgccgtt tctatcactt ttcaggatac ccacgatacg ccagtatttg
     5821 agccgctgcc gggcgaaacg cgcctgtggg gcgacaccga tgtgattggt ctgttcgacg
     5881 ctgaaaccga tatgaacgac gtggtggcga ttctggaaaa ccatccgctg ctcggcgcag
     5941 gcttcgcgca taaaatcgaa caactagaag ataaagactg ggagcgcgaa tggatggata
     6001 atttccaccc gatgcgcttt ggtgaacgac tgtggatctg ccctagctgg cgtgatgtgc
     6061 cggacgaaaa cgccgtcaac gtgatgttag atccagggct ggcgtttggt acgggtaccc
     6121 atccaaccac ctctctgtgc ctgcaatggc tcgacagcct cgatttaacc ggtaaaacag
     6181 tcatcgactt tggctgtggt tccggcattc tggcgatcgc ggcgctgaaa ctgggtgcag
     6241 caaaagccat tggtattgat atcgatccgc aggcgattca ggccagccgc gacaacgccg
     6301 aacgtaatgg cgtttctgac cgtctggaac tctacttacc gaaagatcag ccagaagaaa
     6361 tgaaagccga cgtggtggtc gctaacatcc ttgcaggccc attacgtgaa ctggcaccgt
     6421 taatcagcgt cctgccggtt tcaggcggtt tgctgggcct ttccggtatt ctggcaagcc
     6481 aggcagagag cgtttgtgaa gcttatgccg atagcttcgc actggacccg gtcgtggaaa
     6541 aagaagagtg gtgccgtatt accggtcgta agaattaacc ttcgcatcgc cgtagggtga
     6601 cgcggggcga agtgcgagca agctcacaaa aggcacgtaa atttgccggt tatttacgca
     6661 aatttgcgtg ccaaaatttt cattcataaa gaaaaattga gaacttactc aaatttcttt
     6721 gagtgtaaat tttagtcact attttctaat atgatgattt ttatgagtaa ttatcgcacc
     6781 acgctcattt taaatgcaat tctttgatcc atctcagagg attggtcaaa gtttggcctt
     6841 tcatctcgtg caaaaaatgc gtaatatacg ccgccttgca gtcacagtat ggtcatttct
     6901 taactcatgc gcatcggaca atatcagctc agaaatcgcc tgatcgcagc gcccatggct
     6961 ggcattacag acagaccttt tcggacgttg tgctacgaga tgggagccgg attgacagta
     7021 tccgagatga tgtcttctaa cccacaggtt tgggaaagcg acaaatctcg tttacggatg
     7081 gtgcacattg atgaacccgg tattcgcacc gtgcaaattg ctggtagcga tccgaaagaa
     7141 atggcagatg cagcacgtat taacgtggaa agcggtgccc agattattga tatcaatatg
     7201 ggttgcccgg ctaaaaaagt gaatcgcaag ctcgcaggtt cagccctctt gcagtacccg
     7261 gatgtcgtta aatcgatcct taccgaggtc gtcaatgcag tggacgttcc tgttaccctg
     7321 aagattcgca ccggctgggc accggaacac cgtaactgcg aagagattgc ccaactggct
     7381 gaagactgtg gcattcaggc tctgaccatt catggccgta cacgcgcctg tttgttcaat
     7441 ggagaagctg agtacgacag tattcgggca gttaagcaga aagtttccat tccggttatc
     7501 gcgaatggcg acattactga cccgcttaaa gccagagctg tgctcgacta tacaggggcg
     7561 gatgccctga tgataggccg cgcagctcag ggaagaccct ggatctttcg ggaaatccag
     7621 cattatctgg acactgggga gatgctgccc ccgctgcctt tggcagaggt taagcgcttg
     7681 ctttgcgcgc acgttcggga actgcatgac ttttatggtc cggcaaaagg gtaccgaatt
     7741 gcacgtaaac acgtttcctg gtatctccag gaacacgctc caaatgacca gtttcggcgc
     7801 acattcaacg ccattgagga tgccagcgaa cagctggagg cgttggaggc atacttcgaa
     7861 aattttgcgt aaacagaaat aaagagctga cagaactatg ttcgaacaac gcgtaaattc
     7921 tgacgtactg accgtttcta ccgttaactc tcaggatcag gtaacccaaa aacccctgcg
     7981 tgactcggtt aaacaggcac tgaagaacta ttttgctcaa ctgaatggtc aggatgtgaa
     8041 tgacctctat gagctggtac tggctgaagt agaacagccc ctgttggaca tggtgatgca
     8101 atacacccgt ggtaaccaga cccgtgctgc gctgatgatg ggcatcaacc gtggtacgct
     8161 gcgtaaaaaa ttgaaaaaat acggcatgaa ctaattcagg ttagctaaat gcttgattaa
     8221 aaaggcgcta ctcggcatgg ggaagcgcct tttttatagg tgtcacaaag ggagtgacca
     8281 tgagaacagg atgtgaaccg acccggtttg gtaatgaagc taagaccatt attcacggtg
     8341 atgcgcttgc cgaacttaaa aagatccccg ccgaaagtgt cgatctgatc tttgccgacc
     8401 caccatataa catcggtaaa aattttgatg gtctgatcga agcctggaaa gaagatctgt
     8461 ttatcgactg gctgtttgaa gtgattgcag agtgccaccg cgttctgaaa aagcagggca
     8521 gcatgtacat catgaacagc acggaaaaca tgccctttat cgatctccag tgccgcaagc
     8581 tttttaccat caaaagtcgc atcgtctggt catatgacag ttctggagta caggcgaaaa
     8641 aacactacgg ctccatgtac gaacccatcc tgatgatggt gaaagacgca aagaactaca
     8701 cattcaacgg tgatgctatt ctggtagaag ccaaaaccgg atcgcagcgc gcgttgatcg
     8761 attatcgcaa aaatcctcca cagccataca atcatcaaaa agtaccgggt aacgtctggg
     8821 attttccgcg cgtgcgttat ttgatggatg aatatgagaa ccacccgacg caaaaaccgg
     8881 aagccttact gaaacgcatt attctcgcct cttccaaccc aggcgatatc gttctcgacc
     8941 cgtttgccgg tagctttact accggtgccg tagccgtctc cagcgggcga aaattcattg
     9001 gtattgagat caacagcgag tacatcaaaa tggggcttcg acggctggat gtcgcgtcgc
     9061 attactctgc ggaagaactg gcgaaagtga aaaaaagaaa gaccggcaac ctgtcaaaac
     9121 gaagccggtt aagcgaagtt gaccccgatc tcattgcaaa gtaaaggaag tgtaagcctg
     9181 gttttcagat tattcatttc gtgtatattt cctgccagac ttggttaaac atacacaggc
     9241 tcaggtaatg attcgcaagt attggtggct cgtcgttttc gctgtcttcg tttttctgtt
     9301 tgatacttta ctgatgcagt ggattgaact gctggcaaca gaaacagaca aatgccgcaa
     9361 tatgaactca gttaatccac taaaactggt taactgtgac gaactgaatt ttcaggacag
     9421 aatgtgaatt tacatgacac ttaattcatt cgtttgatga attaatttcg ttatgttttc
     9481 atctggcatg aacattctta atacgttatc gaccagagcg ggggcttgtt tataaagatc
     9541 ataacccgcc atattcatta accagttttg aacaattccg ctgaaggcac cattaataat
     9601 aatcatcaca acatctaaat cgaggttatt tgctacacaa ccttgttgct gacacgcctg
     9661 caatacttcg cggagagtct gcggattgaa gcccatcttt tcgcgtatca ctccctcggc
     9721 cagcatctca tcattaaatt cacatttgtg atataagatt ttcagcaacg cctgctggcg
     9781 gggaattttg gcaatatatt gcaagccgac aatcaatttt tcacgcaatt gttgaaacgg
     9841 gtcatgctct aatccagccg tcaagtgttc ctggattaac tcccgcaatg aaggctgttg
     9901 caaccacatc tcattaaaca gttgagtctt gttttcgaag tgccagtaga tagcgccacg
     9961 cgtaacgtta gcagcgtcgg caatgtcgtt gagtgtcgtc ttgcttacgc catgctgcgc
    10021 aaactgggcg atggcagttt caatcagttc ttgccgggtc ttcagagctt cggctttggt
    10081 tctttttgcc atgattaatt attcaggaaa taaatatatt cgacacagag tgagaaaata
    10141 gcgaaggtta atctatcacc taa
//
LOCUS       DAPHDX010000146        10090 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPHDX010000146 DAPHDX010000000
VERSION     DAPHDX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.148"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(17..634)
                     /locus_tag="RH058_003783"
     CDS             complement(17..634)
                     /locus_tag="RH058_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV3139309.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            901..2400
                     /gene="ansP"
                     /locus_tag="RH058_003784"
     CDS             901..2400
                     /gene="ansP"
                     /locus_tag="RH058_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV3139310.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2515..3576)
                     /locus_tag="RH058_003785"
     CDS             complement(2515..3576)
                     /locus_tag="RH058_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV3139311.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3818..5920
                     /locus_tag="RH058_003786"
                     /pseudo
     CDS             3818..5920
                     /locus_tag="RH058_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(5956..6621)
                     /gene="mcbR"
                     /locus_tag="RH058_003787"
     CDS             complement(5956..6621)
                     /gene="mcbR"
                     /locus_tag="RH058_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV3139312.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6819..7856)
                     /gene="curA"
                     /locus_tag="RH058_003788"
     CDS             complement(6819..7856)
                     /gene="curA"
                     /locus_tag="RH058_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV3139313.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8040..8558
                     /gene="mddA"
                     /locus_tag="RH058_003789"
     CDS             8040..8558
                     /gene="mddA"
                     /locus_tag="RH058_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV3139314.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8555..9004
                     /locus_tag="RH058_003790"
     CDS             8555..9004
                     /locus_tag="RH058_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3139315.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9005..9238)
                     /locus_tag="RH058_003791"
     CDS             complement(9005..9238)
                     /locus_tag="RH058_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV3139316.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9324..9497)
                     /gene="ortT"
                     /locus_tag="RH058_003792"
     CDS             complement(9324..9497)
                     /gene="ortT"
                     /locus_tag="RH058_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV3139317.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9692..9787
                     /gene="yncL"
                     /locus_tag="RH058_003793"
     CDS             9692..9787
                     /gene="yncL"
                     /locus_tag="RH058_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV3139318.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2432 a         2465 c         2460 g         2733 t
ORIGIN      
        1 tcttctggta tcccactcaa ataatttcat tcctttttaa tacttcttgt aatttcggta
       61 actgacatac agcatcagcg atcgcgctga tattctgcgc attatcctga aaccattcat
      121 gccccggccc ccatgtgcgc atggtgcaga gataacagtc caccagcgtt agttgctcac
      181 caaaggcata aggttcagcg gtcaattgtg aattcagcca gatataaagt gatttgcgat
      241 attcaattac attcttcttt aactgttcag gtgcgtcaga tgcccaacgc tcagggtaat
      301 cggcgaaagt gaatgttgga tagacattag caaccagcca gaccaatagc cgttgaaact
      361 gctgacgttc ggcgcgccca accggtggcg caaggtccgg gcgacgatcg agaaccatca
      421 atgcaatcgc cgctgtctcc gtcataatct cgtcattttc cagcgccagt gtcggaacct
      481 gacacaacgg gttcagggtt ttcaataact cgcgtgaagc tccctcgtgg tcaaaaccac
      541 tgacatcaac gaactgataa ggaatatcag ccagtgttag catcagctca ctgattgtag
      601 agccccatcc gggtacgcca tacaccttaa tcatatgccc ctcacaatct tttaaagatt
      661 aagtgtagga cagtacacag gttaaccagc aaaaggattt gtcatttttt cgtcagttgt
      721 attaatcccc ttcaggattg ttaggggatg ttttgcatcg tttatcgata acgggtagga
      781 tgctgcgccg atagttttat acccataaag aataatggtg ataactatca tcgccaggat
      841 gaataaacat tgttcatggc aacttatatg actttttcat taaagcaatc agggagagca
      901 atgagtaaac acgacaccga cacttcagat caacacgccg cgaaacgccg ctggcttaat
      961 gcccacgaag aggggtatca caaagcgatg ggcaatcgcc aggtacagat gatcgccatt
     1021 ggcggcgcga ttggcaccgg cttgttttta ggtgcaggag cccgactgca aatggcgggt
     1081 ccagcattgg cactggttta tttaatttgt ggcttgtttt cgttttttat tctgcgtgca
     1141 ttgggtgagc tggtgctaca ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag
     1201 tttttgggtg agaaagccgc ttatgttgct ggctggatgt acttcatcaa ctgggcgatg
     1261 acggggattg ttgatattac cgccgtcgct ctgtatatgc attactgggg tgcgtttggc
     1321 ggcgtgccgc agtgggtctt tgcgctcgct gcacttacca tcgttggcac catgaatatg
     1381 atcggtgtga aatggtttgc ggagatggag ttctggtttg cgcttattaa agtgctcgcc
     1441 attgtgacct ttttggtcgt gggtacagtg ttcctcggta gtggtcagcc gctggatggc
     1501 aacaccactg gctttcattt aattaccgat aatggcggct tcttccccca cggtttgctg
     1561 cctgctctgg tgttgattca gggcgtagtg tttgcttttg cctccattga aatggtgggt
     1621 acagctgccg gagaatgtaa agatccgcag accatggtgc ctaaagcgat taacagcgtg
     1681 atttggcgta ttggcctgtt ttacgtcggc tccgtggtgt tgctggttat gctattgccg
     1741 tggagcgcgt atcaggcggg gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg
     1801 ccatatatcg gcagcattat gaacattgtg gtgctgaccg ctgccctctc cagcctgaac
     1861 tcaggtctgt actgcaccgg acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg
     1921 agttttatgg cgaaaatgag tcgtcagcat gtgccgtatg ccgggattct ggcgacacta
     1981 gttgtgtatg tcgtcggcgt attcctcaac tatctggtgc cgtcgcgcgt atttgagatt
     2041 gtgttgaact tcgcgtcgct gggaatcatc gcttcatggg cgtttatcat cgtgtgccag
     2101 atgcgcctgc gtaaagcaat taaacaaggc aaagcagcgg atgtcagttt taaactgcct
     2161 ggcgcgccct tcacttcctg gctgacatta ctgtttttac tgagtgtcct ggtgctgatg
     2221 gcgttcgatt acccgaacgg gacttatact atcgcggcgc tgccgattat cggtattctg
     2281 ctggttatag gctggtttgg tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca
     2341 gtcgtcgagg aagatgaaga aaaacaggaa attgtgttta agcctgaaac ggcgagctaa
     2401 ttgcaaaatt gcccggctac gtgccgggca aatttagatt actgacgaag gtgtgccgca
     2461 tgcggcgtga taaaagtcag gcaagtctct ccgttttcac cctcgccaca tctattacag
     2521 cgcaatacga atcacatcgt ccggctgggt agcttcctgc tgtttagtgg atttttgttt
     2581 cacactgaca tacagcgttt tgccatcggc agacagcgcc aggctgtttg gatgagtcgg
     2641 cgtatcgaac gttttcacca ctttatagct tttcgcgtca atcacactga ctttacctgc
     2701 ctgacgatgc gttacgtagg cttcattacg cgctgggtta aacagcacag ccagtgattc
     2761 tggtgccgca accttcgcca gaatattgcc attacgggta tcgaccacta acacttctgc
     2821 ggctttagaa tcggtgataa atgcacgctg cctggcggta tcaaggctaa tgttgataaa
     2881 gaagtgctct ttgccgtcat ccagcagctt tttacggctg aggattttat tgtcggcggt
     2941 gtcgatggta atcaattcgc cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc
     3001 cagcgccaga ccggtactca ttttaccggt gttctggatg gcggttttca gtttgatatt
     3061 ctcgccatca acgacccaaa tcacgctctc tttaccaata ccactgatgt aaacggtgtt
     3121 cgtggcatca tcagctacca gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt
     3181 acgatcatcc agcaccagac ggcctttcac ctcgcccgtt ttggcatcta tcgccgtgac
     3241 cgcgctgttt acggtgttac caaaccacaa cgtctgagtc gtgttattga tggtggcacc
     3301 aaacggcttg agatcgttat ggatcgcctg cgtcacttcc agagtgaccg gatcaagacg
     3361 ataaaccacg ccacctttat ccagtttgcg gctttgcgaa gtggcgagcc acagtgcgtt
     3421 ttcttgctgg ctataagcca tttcgtaggc acctttacct accgctttac gcagcatttc
     3481 ttctgcggcc tgcgtactga atgatgaagc aacaagcaat gaacctaaca gtaatgaacc
     3541 acgcaggcgc gatgaaaaca gatgacgtaa atgcatgacg actccctttg atgaaccgat
     3601 atttttgctg cttcacaatg cttcgctaag cgtaatcatc gttacgctct tgttataaat
     3661 gggaatggta atcattattt tctcaaatgt ggagcatttc ttgccgttac gactacaatt
     3721 ttctcaatta cttaacgtat cgtgccgttt cgttttccat aaatttacaa tttcattacc
     3781 ataacggcgt tttcccattc ttaacctggc ttttttcatg aagatttttt ccgtccgaca
     3841 gaccgttttg cccgcactgc ttgtcctttc ccccgttgtt tttgccgctg atgaacagac
     3901 tatgattgtc agtgccgcac cgcaggtggt ttcagaactg gataccccag cagcagtaag
     3961 cgtggtggat ggcgaggaga tgcgcctggc aacaccgcgc attaacttgt ccgaatcact
     4021 gaccggcgtg cctggtttgc aggtacaaaa ccggcagaac tatgcgcaag atttacagct
     4081 gtcgattcgc ggatttggct cccgctccac ttacggtatt cgcggtattc gcctgtatgt
     4141 ggacggtatt cccgccacca tgcccgacgg gcaagggcaa acatccaaca tcgatttaag
     4201 cagtgtgcaa aatgtggaag tgctgcgtgg ccccttctct gccctgtatg gcaacgcgtc
     4261 tggtggggta atgaatgtca ccacccagac cggacaacag ccaccaacca ttgaagccag
     4321 tagttactac ggcagttttg gcagctggcg ctatgggctg aaagcaacgg gcgcaacggg
     4381 agacggcaca cagcctggcg atgtcgatta caccgtctca accacgcgtt ttacgaccca
     4441 cggctatcgt gaccatagtg gcgcacagaa aaatttagct aatgccaaac tgggcgtacg
     4501 cattgatgaa gccagcaaat taagtctgat tttcaatagt gtggatatca aagcagatga
     4561 cccaggtggg ctaaccaaag cagaatggaa ggctaatcca caacaagcgc ctcgtgcaga
     4621 acagtacgac acgcgaaaaa ccatcaagca aactcaggct gggttgcgct atgagcgtag
     4681 cctgagcgcg caagatgata tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca
     4741 gtaccagtca atcccgatgg ccccccaact taacccgtca catgcgggcg gcgtgattac
     4801 cctgcaacgc cattaccagg ggatagacag ccgctggaca caccgtggtg aactgggcgt
     4861 tccggtcacg ttcactaccg gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta
     4921 caataacttc cgcctgaata gcggcatgcc ggagtacggg caaaaaggtg agttgcgtcg
     4981 cgacgaacgc aatctgatgt ggaacatcga tccctattta cagacgcagt ggcagctgag
     5041 cgaaaaactg tcgctggatg ctggcgtgcg ctacagctcc gtgtggtttg attccaacga
     5101 ccattacgtt actccgggta acggcgatga cagcggtgat gccagttatc ataaatggct
     5161 acctgccggt tcgttaaaat atgcaatgac cgatgcctag aatatctatc tggcagccgg
     5221 gcgaggtttt gaaacgccga cgattaatga gctgtcttat cgtgctgatg ggcaaagcgg
     5281 tatgaacttt ggtttaaaac catccaccaa cgatacaatt gagatcggca gtaaaacgcg
     5341 tattggtgat gggctgctta gtctcgcatt gtttcagacc gacactgatg atgaaattgt
     5401 tgtcgatagc agtagcggtg ggcgtacgac ttacaaaaat gccggaaaga cccgtcgtca
     5461 aggcgctgaa ctggcatggg atcaacgttt cgcaggagat tttcgcgtaa acgcgtcctg
     5521 gacctggctt gatgcgacct atcgcagcaa tgtttgcaat gaacaggatt gtaacggtaa
     5581 tcggatgcca gggatcgccc gtaatatggg ctttgcgtcg ataggttatg taccggaaga
     5641 tggttggtat gcaggcacgg aagcgcgtta tatgggcgat attatggcag atgatgaaaa
     5701 tacggcaaaa gcgccgtctt atactctcgt cggcttattc accgggtata aatacaatta
     5761 ccacaattta actgtggatt tatttggtcg tgtcgataat ttattcgata aagaatacgt
     5821 tggttctgtc attgtcaatg agtcaaacgg gcgatattac gaaccttcgc ccggacgaaa
     5881 ttatggtgtc ggcatgaata ttgcgtggag atttgagtaa ttaccttgat gcccggtatt
     5941 tgccgggcat ttactttaac gattgtattg ctggtataaa atagcaatat tttgttgcat
     6001 aatttcagca agacaatgtc tgcatccctc tttgtctttt gcttttaacg cagcgagtaa
     6061 cagatgatag ttttctatat gctcccgtaa ttccgctgga ttaatcgctt catagagata
     6121 atgtaaacca ggccccatcc tgacccacag ttgctcaatc atctcacaca ggatgggcat
     6181 atttgagcga tgataaatcg ctaagcgaaa tagcctgttt acattaatga tttgttccat
     6241 gtcgcccttt tcttgcgcct gctgtaattt ctcgagcaat tcctggagtt ccgccaggtc
     6301 ttgcggggtg aggttttcaa cagccagagc aactgccatt aattccagtt cgtagcggat
     6361 ccgattgatt tcgtctaatt gacgtttccc cacttccgga actgtaaatg cttgtgcagg
     6421 tgcgacagaa agcgcattca ccgaaaccag acgtaataat gcttcacgca caggtgtaat
     6481 actcatacct aattgctccg ccagattttt agtaatcagg cgtgcgccag gttttaatgc
     6541 gccaatacta agctgatgtt tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt
     6601 catttttccc attccaggca ttttaattcc ttcgcgtgtt ttctgtccaa ccctgcctgc
     6661 acggcagaag gagttcacct ggtgtattaa agtgaataat cataaaccac ccacctttgt
     6721 tgttattagc agcagaacaa tgacgtggtt agttaattaa agctgaaaat aagctttttg
     6781 cctggatttt tatggcggct tacgccgccg ttgaaacttt aatcatcacc cgcgacgcgg
     6841 atcaccactt tgccgaagtt tttacccttc agcaggccga taaacgtctg tggcgcattc
     6901 tctaaaccat cagtaatatc ttcgtggtag tggattttat cctctttcac ccattgcccc
     6961 atctccttct gaaactcatg gatgcggtga ccataatcct gagcgataat aaacccttgc
     7021 aagcgaatac gttttttcag caccgttgcc atcaacaggg gtaaacgatc cggaccgggt
     7081 ggtagctctg tagcgttata gctgctcact aagccacaaa cgggaatgcg cgcagatgta
     7141 ttaagtaatg gtagcactgc atcaaatacc ttaccgccca cgttttcata atagatatca
     7201 ataccttttg ggcacgcttt caccagttgt tcggcaaaat catccgcgtg gtgatcaaga
     7261 caaacatcga agcccagcac ctcgatagca tggcggcatt tttccgcgcc accggcaacc
     7321 cccaccaccc tgcaaccttt aagtttgccg atttgcccca ccgttgctcc cacaggacct
     7381 gtcgccgcag ctaccaccaa cgtttcgccc tctttaggct gaccgatatc cagcagcccc
     7441 atataagcgg taaagcctgg catccccagc acacccagcg accacgatgg attttgcgga
     7501 tgatcgccaa gtttcaccag atcatcacca ctggatatgt catagtcctg ccatccactg
     7561 tagcccagca cccagtcgcc aggctgataa tcaggatgat tcgactccac aacacggctc
     7621 accgtgccgc cgaccatcac gccgccaata tcaacaggcg gtgaataaga tggctcatcg
     7681 ctcatacgtc cacgcatata cgggtccagg gacaaataaa ctgtgcgcag taacacctgt
     7741 ccttcacccg gtgtggcgac atcatcttct tcaagacgga aattctccgg aaccggtgcg
     7801 ccatgtggac gcgaggccag aacccaacgg cggttacgct gcttttgttg ccccataatg
     7861 ctctcctgtg cttttggcat gaatccatca atagtagcag gctcgcagga ttggttttgt
     7921 ttgcagccgt cacatcccct ctgcaacacg caataatttc atcgaggaat ccgttgcttc
     7981 gggctgtttt cgatagtatt gataacatca tcatcatcat tattttattg aggccagaca
     8041 tgtccatccg ttttgcccgc aaagccgact gtgctgccat tgcggaaatt tataaccacg
     8101 ccgtgttgta tacggcggct atctggaatg accaaacggt ggatgcagat aatcgcattg
     8161 cctggtttga agcgcggacc atagcaggtt atccggtgct ggtaagtgaa gaagatagcg
     8221 tagtgacggg atatgcctcg tttggcgact ggcgtagttt cgatggtttt cgccataccg
     8281 tggaacattc ggtttatgtc catcccgatc atcagggcaa aggtctgggg cgtaaattgt
     8341 taagccgatt gattgatgaa gcgcgggatt gcgggaagca tgtcatggtc gccgggatcg
     8401 aatcgcaaaa tcaggcctcg ctgcatctgc accagtcgct gggatttgtc gtcaccgcac
     8461 aaatgccgca ggtgggcact aaatttgggc gctggctgga tctgacattt atgcagttgc
     8521 aactcgacga acgcactgaa ccggacgcga ttggatgaat cagtcgctca cccttgcttt
     8581 tttgattgcc gccggaattg gtctggtagt ccagaacacg ctgatggtgc gcatcaccca
     8641 gacctcctct accattctca tcgccatgtt gctgaactca ctggtgggga ttgtgctctt
     8701 tgtttctatt ttatggttta aacaaggcat ggcggggttt ggcgaactgg tgtccagcgt
     8761 gcgctggtgg acacttatac ccggcttgct gggatcgttt tttgtcttcg ccagtatcag
     8821 cggatatcag aatgtgggag cggcgacaac tattgcagta ctggtcgcca gccagctaat
     8881 tggcggtctg atgctggata tcttccgcag tcatggcgta ccactacggg cgttgttcgg
     8941 cccaatttgt ggcgcaattt tacttgtggt tggcgcctgg ttggtagcca gacgctcatt
     9001 ttaatcagag gatggtgccg cctttggtta actgttcgtg gcgcgcttcc atatcttctt
     9061 tatgcttgcg accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg gtgtagcgat
     9121 cttcccgact taactccgcg tcatcgctca gggcgattaa taattcgttc atatgggcaa
     9181 tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga tgcgacatac
     9241 tttctcctta accacttgtg aaatttatcg attcatccaa atgatataag tctgcgtggt
     9301 gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca acagcgccac cgggaaactc
     9361 atcggccatg ttgcccccac cagaaaggcg cttaagaaac ggatgcgttt cttatcgtga
     9421 gaaagaaacc aggtgataag aaatgcgatt gctgccataa ccgcataaaa aacaagcata
     9481 tgttgataga gagacatgta cgggcgtcca gccagatgaa taaaacgcgc gcaaatatgc
     9541 gtcatttttt gacgtgcatc aagttttttc accagtttga gaaaaaatca tcaaacagca
     9601 acttgccctt atccatcttc agaatgagta ttattgcgga ttttcttaac ccgtactata
     9661 cacatagggg caatgataaa aggtggcaaa aatgaatgtt tccagtagaa ctgtagtact
     9721 gataaatgtc tttgctgctg ttggtttgtt tactcttatc tctatgagat ttggctggtt
     9781 tatttgatgt agtgattttg cacgcagcac gtactgtcag agaaatcaac tctctgacag
     9841 tacgcaagat cttctcatac tctccaggtt tacaacatta tccttaatat ttacttcttt
     9901 ttgctggcat ctacattgaa atgagtagaa aacatccccc tactgtcgtc tatacaaaca
     9961 gcttactgat tcaaacggaa tcttgttttc acatttttct atcatgaata ctttatacgc
    10021 gcgggagtct ataaatgctt tatactttca aggaataacg taaatataaa aagatggcgt
    10081 aaagtatatg
//
LOCUS       DAPHDX010000147        10027 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPHDX010000147 DAPHDX010000000
VERSION     DAPHDX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.149"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..81
                     /locus_tag="RH058_003794"
     tRNA            6..81
                     /locus_tag="RH058_003794"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="RH058_003795"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="RH058_003795"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /locus_tag="RH058_003796"
     CDS             complement(467..1225)
                     /locus_tag="RH058_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV3139319.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /locus_tag="RH058_003797"
     CDS             complement(1233..2336)
                     /locus_tag="RH058_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV3139320.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /locus_tag="RH058_003798"
     CDS             complement(2346..3527)
                     /locus_tag="RH058_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV3139321.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="RH058_003799"
     CDS             complement(3595..4620)
                     /locus_tag="RH058_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3139322.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPAVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="RH058_003800"
     CDS             complement(5051..5272)
                     /locus_tag="RH058_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139323.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8629)
                     /gene="acrF"
                     /locus_tag="RH058_003801"
     CDS             complement(5525..8629)
                     /gene="acrF"
                     /locus_tag="RH058_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV3139324.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8641..9798)
                     /gene="acrE"
                     /locus_tag="RH058_003802"
     CDS             complement(8641..9798)
                     /gene="acrE"
                     /locus_tag="RH058_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV3139325.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2653 a         2587 c         2422 g         2365 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttttgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgatt taggatgcgc aaaaaattca tcaggtgcag
      541 cttgttccac tatctcccca cgatccataa aaattacccg gtcagcgacg gtacgtgcaa
      601 accccatctc atgtgttaca cacaacattg tcatacccga ctgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga cgttggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgaacgcg caatggcaac gcgttgctgc tgaccaccag
      781 aaatttgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgccggac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg tgggatcaac ggttgcctgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc gcctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatattgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacaatacg gtaatcagcg gtacgccgcg ccagaactcg
     1741 ataaaaatga ccgataagat acgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaacggatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tccagaccaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcaccgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caggttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccaattagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc tgggcgcgag ggctgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttcaacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggcgg aataatcact
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc cagcgcggat aatctcggcg
     2701 ataaatgcag atgtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagcctcgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccaatgatca acactgcggc gatcggccag
     2881 gtgcggcgca gttgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggata gttttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cctaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgcccgt tacctcacac cgacttaacg
     3601 cacgggcggc gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc cagcttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac tgccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagctgggtt gcttaatttg atgcgcaggg catacagttg
     4021 cgactgatcc gaggccagcg tatcgcagcg accagattcc agtgccttcg ctgattcgtc
     4081 agagcgatcg aaagtcaccg gtgtgtactt catattgttt gccttgaaat agtcggcaac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 ggtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagcggt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttacctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcccga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc cacattttgg cgcaaaatat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgtgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacgcga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga actatagaaa gcttttaaag agtgagcaga agaatatctt
     5461 attaattttt atgggtttca ctggaaataa taaaggcacc cgaaagcgcc tttatgtttc
     5521 tgatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccacagcg ttctgcgcgc
     5641 cactgccggc accgttactg atagctagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccatcag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcccacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgttgccgc cagtagcacg ccgacaatcc ctaacggcac taccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg caaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggccgag aatggcacca
     6241 tttcgccgtt ggcgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cgcgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc caacgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattag
     6481 gacgcacgct gactaagctg gcaggatgtt gcgccgccat gccaagcaac tggttacggg
     6541 cctgggtcag ggcatcgtga cccagcccag cctgatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ttccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcactat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc ggcgttttgt gcctggccgc
     6781 tgaagctaaa gccgttaacc gtaaagacac tttcaacgtt cgctttctcg ttcttcagat
     6841 agtaatccgt cacttgatcc aacacttttt gcgtccgctc ttgcgtcgcg ccagcgggta
     6901 actgaatcat ggtcagaaag acaccctgat cctcttcagg taagaaggaa gacggaagac
     6961 gtaaaaacaa caccaccatt cctgcaacaa tcagcgcata gatcagtaaa tatcgtcctg
     7021 tggaaccgag gattttgccg acgctgttgg tgtagtggtt aacgctatga tcgaaggtgg
     7081 tattaaacca gccgaagaaa ccacccttat tttcgtgatg ctcagctgag gtgggtttga
     7141 gcagtgttgc acataacgca ggggtaagaa tcaatgccac cagaacagaa agcgccattg
     7201 ccgaaacgat ggtgatagag aactggcgat aaattgcccc agtagaaccg ccgaagaatg
     7261 ccatcggaat aaataccgct gacagcacca tcgcgatacc caccagtgcg ccctgaattt
     7321 gcgacatcga tttttccgtc gcttcttttg gcgggagctt atcctccatc atcacgcgct
     7381 cgacgttctc caccaccact atcgcatcat cgacgagcag cccgatggca agcaccatcc
     7441 cgaacatcgt tagtgtgttg atggagtaac caaaagcggc gaggatggca aacgtcccta
     7501 ataacaccac gggtaccgca atggtgggga tcagcgttgc tcgcatattc tgcaagaaca
     7561 gatacatcac caggaacacc agcataatgg cttcgaacag cgtttttacc acttcgtgaa
     7621 tagaaagctg gacgaatggc gtggtgtcat aagggtagag aaccttcatt ccctgcggga
     7681 agaatggctg taattccgcc agttttgcct taatggcttt cgcggtatcg agagcattcg
     7741 cgccggttgc cagcttaatc cccaggcccg ccgccggttt tccgttgata cgagcgataa
     7801 cgttatagtt ttcaccgcca agttcaactc gtgcgacatc tttcaggcgt accaccgagc
     7861 cgtcactgtt tacgcgcagg gttactttgc cgaattcttc cggatttttt aaccgcgtct
     7921 gagcaataat tgaggcgttc aactgttgcc ctggtaacgc tggcgttccg cccaactgtc
     7981 cggcagcgat ctgatcgttc tgtaccttca actggttaat cacatcaacc ggtgtcagtt
     8041 tatatttgtt tagcagatcg gcgtccagcc agatacgcat cgcatactgt gcgccgaaaa
     8101 gctgtacgtc accgacgcca ttcagacggc taagcgtatc tttaacgtta gaggccacat
     8161 agtccgagat atcgtcctgt gtggtgtctg ggttatcaga gacaaagccc gccaccatca
     8221 aatagctgct actggacttt tcaacactga tcccctgctg ctgaacctcc tgcggcagca
     8281 acggcgtggc gagctggagt ttgttctgca cctgcacttg cgcgatatca ggatcggtcc
     8341 cggactggaa ggtaagggta attgtcacgc taccggcgga atcgctggtg gaggacatat
     8401 acatcaggtt atcgataccg ttcatattct gttcgataac ctgcgtcacc gtatcctgca
     8461 cggtctgcgc atcagcgccc ggatagtttg ctgaaacaga aaccgcaggc ggtgcaatgg
     8521 ttggatactg agcgacgggt aattgtagga tcgccagtgc gcccgccatc atcagaataa
     8581 tggccagcac ccatgcaaat atcggtcgtc gaataaaaaa gtttgccatg tcagattacc
     8641 ttacttcgat gcagtatctg cgggggtatc ggtagtggct ttaacctgct ctcccggacg
     8701 cgctttttgc aggccgctga caatgacttg atcgccagat ttcagacctt cgctaatcaa
     8761 ccatttatcg ccaatcgcct gactggcaac gacagggcgc gcttccacct gacttttatc
     8821 gttaacaatc agcacggttg catcaccacg cggtgtgcgg ctaacgcctt gttgcgggat
     8881 aagaatggcg tcaggttgga cgccttcatc aatccgtgcg cgcacaaaca tacccggcaa
     8941 aagcgtatgt tgcgggttag ggaagacggc acgtagggtt atggagccgg tgctttcatc
     9001 aacggtcaca tcagagaatt gcagcgtacc tttcagggga taggtttgac cgttttccat
     9061 gaccaactct acgttgctgg tggcgttttc cttatgcaaa tttccttgct ctacggattg
     9121 cttcagcctc ataaaatcgt tgctggattg ggtcacatca acgtagatag gatcgagctg
     9181 ctggacagtc gccagttcag tcgtttgccc attagtgaca agagcgcctt cggtcacggt
     9241 cgatttgccg atacgtccgc taattggcgc agtcactttg gtataagcga gattgatgcg
     9301 agcgctttcg actgtggctt ttgcggcaat cacggcggca tcggcctggc gagcatcagc
     9361 aatggcctgg tcgtactcct gctggctgat gtatttcgta cccacgagcg gaacgtaacg
     9421 ttttaccgtc aaatgcgcga tggcggcggc ggcttcactt ttcgccagtt cgcctttcgc
     9481 gctgtcataa tttgcctgat aggtcgcggg atcgatctgg tacagggact ggcctgcttg
     9541 tacatcgctg ccttcagtga aattacgatt cagtacgatc ccgctaacct gtgggcgaac
     9601 ttcggctata cggtaagcat tggtgcggcc tggtaattca gtcttaactt ctaacggggc
     9661 cgttttcaca atatgaacgg taacctgcgg ttcaccgacg tgagctttct cttctccctt
     9721 atcgttacaa ccggcgatta aagccgcgga gatcagaata aaggagggca ggaggaaaaa
     9781 cctggcatgt ttcgtcatta ctattcctca aaaaaccaaa agcgcgttat ttacccagaa
     9841 aggcaacgcg ttaactcgca gaaagaaaaa tacagtttgc tatcctacaa attatcattc
     9901 gtcgatgtaa ggaatagtta tgaatactgg cgtctcaagg cacataaaca caaaaaaaga
     9961 ttaatattct actgttttat tttgacgcgg gttgaaagag gcagaattaa aacctcgtaa
    10021 attgaaa
//
LOCUS       DAPHDX010000148         9948 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPHDX010000148 DAPHDX010000000
VERSION     DAPHDX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.150"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            204..1298
                     /gene="ddlA"
                     /locus_tag="RH058_003803"
     CDS             204..1298
                     /gene="ddlA"
                     /locus_tag="RH058_003803"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV3139326.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1322..1534)
                     /locus_tag="RH058_003804"
     CDS             complement(1322..1534)
                     /locus_tag="RH058_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV3139327.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1794..2102
                     /locus_tag="RH058_003805"
     CDS             1794..2102
                     /locus_tag="RH058_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV3139328.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="RH058_003806"
     CDS             complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="RH058_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV3139329.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="RH058_003807"
     CDS             complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="RH058_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV3139330.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4840..5997
                     /gene="ampH"
                     /locus_tag="RH058_003808"
     CDS             4840..5997
                     /gene="ampH"
                     /locus_tag="RH058_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV3139331.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="RH058_003809"
     CDS             complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="RH058_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV3139332.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6709..9711)
                     /gene="ehaB"
                     /locus_tag="RH058_003810"
     CDS             complement(6709..9711)
                     /gene="ehaB"
                     /locus_tag="RH058_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HDV3139333.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTI
                     ASWDDVALAVVAHPNTDNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDADG
                     EVDTNGWDGTDRLDLTLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATAT
                     LIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTGSQWDTTKTSL
                     IDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEV
                     SAGWAVGDAALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNI
                     ESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEV
                     DNNGDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAY
                     TYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNG
                     GAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDR
                     SGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWG
                     FGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAG
                     YTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNF
                     SAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2558 a         2459 c         2364 g         2567 t
ORIGIN      
        1 tggccattta agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac
       61 atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
      121 caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      181 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      241 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      301 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      361 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      421 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
      481 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      541 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      601 tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc
      661 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      721 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
      781 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
      841 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
      901 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
      961 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1021 ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1081 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1141 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1201 aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg gcgctggagc
     1261 gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc cgataatatt
     1321 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaacccc gccagccaga
     1381 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1441 ccagtagccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1501 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     1561 tccttgcata ttctgtaaca accgtctgtg gtgtagacaa catttttgta attgttaata
     1621 aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt cgggaatatt
     1681 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1741 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1801 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1861 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     1921 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     1981 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     2041 taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     2101 aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat tcctgtttga
     2161 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2221 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     2281 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc
     2341 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     2401 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     2461 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg cgttctgaaa
     2521 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     2581 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     2641 actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     2701 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc
     2761 aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2821 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     2881 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     2941 ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc
     3001 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3061 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3121 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     3181 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     3241 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3301 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3361 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3421 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     3481 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     3541 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     3601 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     3661 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcaa
     3721 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3781 gacaattggc agctccggca catgtgcgga gagcgttacc agcaccggca ggaaggcgat
     3841 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     3901 catggtgtct tcctgcacac gctgtgcggc cccttcgata tgacgcagtt gttgccagtt
     3961 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa
     4021 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4081 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     4141 atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa accaggtgac
     4201 gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     4261 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4321 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4381 cgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     4441 ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4501 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca
     4561 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4621 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     4681 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4741 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4801 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4861 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     4921 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     4981 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc
     5041 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     5101 ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag
     5161 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg
     5221 acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     5281 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgaacaacg ctggaaatac
     5341 ctttctacgg cgaagctaaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     5401 ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5461 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     5521 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     5581 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     5641 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     5701 tatcagcgcg cgcagtttac caaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5761 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5821 acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     5881 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     5941 aatgacctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     6001 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6061 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     6121 atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga
     6181 taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca cgtcgcgtaa
     6241 ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga gttgattttg
     6301 ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     6361 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6421 agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa cgccctctaa
     6481 aataacgaaa gtatcttcgc tattgacatc actggaaaat ataacttgct tttcattatt
     6541 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6601 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6661 gggcattatt tagattaaat taccagaatt tcttcggagt gaagaatatt accaggtata
     6721 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
     6781 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
     6841 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
     6901 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctggtctt cgctgtaggt
     6961 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
     7021 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaatgct
     7081 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc
     7141 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
     7201 agacaagtcg ttgttgaagt gagagtaact caagctacca tcaacaaaga cgttgttcgc
     7261 gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatccacct gaccagaacg
     7321 gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact tagcgttgtt
     7381 accgtcaatt ttggtatcaa caccgaccat gatgccgtta acatcctgat catagttgat
     7441 ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc cgccgttatc
     7501 agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt ccaggttcca
     7561 gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg tcagctccat
     7621 ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg cacccaggtc
     7681 agctttgtta gcagcagaga aggtcgcgtt gctgtttttg tcgttgacgt agatcagctc
     7741 gttgccgttg tagtcagcga tagaaccagc accagttgcg ttgtcgatac gaactttgta
     7801 gttaccggtt gcagctgcaa catggtcacc gttgttatca acttcgttct gaccagcttc
     7861 gatagaagca gtgttgtcgt tatcgccgtt accgttaatg gtcaggtgac catcagagtt
     7921 cattgcgatt aagccataac cgtagtttga cttggtagta tcgttggtac ggtcgttggt
     7981 caggtctgca tcaagtacga agcggttgct ttcgatgttg aacacaccag cgtgaatgtt
     8041 accactggta tcagtagtgc tggtcaggtt cagagtatca acctgcagag cattcgccgc
     8101 agtgttacct acatccagaa taccgtcgtt agtcactttg atgttgttgg catacagagc
     8161 agcatcacct acagcccaac cagcagaaac ttcatcagaa atggttacgg tactgttgtc
     8221 gacagtcagt gaatcagttg caacatgacc atcttcgttg atgttcagcg cagaaagacc
     8281 tgtcagagag atagagtcag aaatcagcgt tgaatcagca acattaacag ttgaaccact
     8341 gttgatgctc agagtatcaa tcagagaagt tttggtggta tcccactgtg aaccggtatt
     8401 cagggtcaca ttgaacagac cgctctggta aacctgatta ccagtaatat gatcgaattc
     8461 actgtaactt gtatcatcgt tttcaacacc aacggttgat aacggccaca ggctattatc
     8521 ctcaatatca atcagagttg cagttgcctc ggtgccaaca ccgtatgtag cgatgtcacc
     8581 tttaccatct ccatcaacat caatagaacc agtttgatga acagattgtg ctgcccctac
     8641 ccatttacta ccgttattta atgtcagatc caggcgatct gttccatccc aaccgttggt
     8701 gtctacttca ccatcagcat cgcgataact atctgcacca cgtgggaaga agttttcatc
     8761 aaagttgcta gagaagatta catcgcccat caaagtagag ttactaaagt cagcggtggt
     8821 ctgcatagca ttatcagtat tcggatgtgc aactacagct aatgcaacat catcccagct
     8881 tgcaatagtg ctatcagcaa caccatctcc gtcagtatct acggtgacaa agttagattt
     8941 accactataa tcgctggcat tacctgtatt accgaaccaa ccagtagtac cttcatcagt
     9001 ccatgaacca gaggtcacgg ttgaatcttt aacagtgatg gtgttattaa acacttcgct
     9061 gctgtttacg ccagtgctga cagtgttgtc gtcaatatct tcccactcat aaccctgagt
     9121 caacgtaata cccgcaacgt gagagttatt agtgatggac agatcaactt cctgatccag
     9181 agtaatagcc gtaccaatgt tgtaaacgtc tacaacatga gtatcagcgg cattattgta
     9241 agtgccgtta tatgtgtagt gttcgtagtt gtcgtcgatc gttgaattat caacagtcag
     9301 cgccagacgg tcatggtagt aatcatccgc acgatcagca caatcagttg tcatgcactc
     9361 ggaataaatc ataccgtgaa tagtgctgtt tttgattgtc agactattgg cgttggtatt
     9421 ggtagaaata ccgtcgtcca gatagtaggt agaaatcacg ccgttaacgg tagcgttatt
     9481 aatgaccggg tagatatcgc cgttgtaata gctatcagcg gcatagttgt tccagcctac
     9541 ataaccatca tagtaaacgt catcagcata ggtggcgtcg ttgtagtgat ggaaagtatt
     9601 gtaagtttga ccagaaatat cgttggtcgc tgcattagcc tgagaggtga tagccagagt
     9661 gcaagccaat gctaattgtg atactacaag tttctttttc caggagtgca tttgtcatcc
     9721 ctcctcaggg acgtaataag ttgatccatc aattatcaat gcatagaaaa catattatgt
     9781 ttcgcgctaa cgattatgct gcgttacaga cgaatggttc aattattctt tcccctaagt
     9841 ccgaatgtgg acatatataa tttatggaga aaaacacgca tcaaattgat aaaaggacga
     9901 tatttcaaca tgttgcagat aatgttattt ttgatacata cgcgttat
//
LOCUS       DAPHDX010000149         9832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPHDX010000149 DAPHDX010000000
VERSION     DAPHDX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.151"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            10..582
                     /locus_tag="RH058_003811"
     CDS             10..582
                     /locus_tag="RH058_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDV3139334.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            630..1361
                     /locus_tag="RH058_003812"
     CDS             630..1361
                     /locus_tag="RH058_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3139335.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1386..3908
                     /locus_tag="RH058_003813"
                     /pseudo
     CDS             1386..3908
                     /locus_tag="RH058_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3919..4992
                     /locus_tag="RH058_003814"
     CDS             3919..4992
                     /locus_tag="RH058_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3139336.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDISAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5240..6280
                     /gene="pstS"
                     /locus_tag="RH058_003815"
     CDS             5240..6280
                     /gene="pstS"
                     /locus_tag="RH058_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV3139337.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6367..7326
                     /gene="pstC"
                     /locus_tag="RH058_003816"
     CDS             6367..7326
                     /gene="pstC"
                     /locus_tag="RH058_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV3139338.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7326..8216
                     /gene="pstA"
                     /locus_tag="RH058_003817"
     CDS             7326..8216
                     /gene="pstA"
                     /locus_tag="RH058_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV3139339.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8307..9080
                     /gene="pstB"
                     /locus_tag="RH058_003818"
     CDS             8307..9080
                     /gene="pstB"
                     /locus_tag="RH058_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV3139340.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9095..9820
                     /gene="phoU"
                     /locus_tag="RH058_003819"
     CDS             9095..9820
                     /gene="phoU"
                     /locus_tag="RH058_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV3139341.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         2524 a         2269 c         2504 g         2535 t
ORIGIN      
        1 ggagttttta tgaagcgtaa tattataggc ggtgcattca ctctggcatc tctaatgctg
       61 gccgggcatg cactggcaga agatggtgtt gttcacttcg tcggtgaaat tgtcgacact
      121 acttgtgaag ttacctccga tacagccgat caaattgtcc cactgggtaa agtcagtaaa
      181 aatgcatttt caggtgtagg tagtctggcg tcgccacaga agttcagtat taagcttgaa
      241 aattgcccgg caacgtacac tcaagcagcc gttcgttttg atggtacaga agcgcctggc
      301 ggcgacggcg acctgaaagt gggtacgccg cttacagcag gcaaccctgg tgattttacc
      361 ggtacaggac aagcgattgc ggcaaccggc gttggtattc gtatttttaa ccagtccgat
      421 aattcgcagg ttaaacttta taacgactct gcttataccg ctatcgatgc tgaaggcaag
      481 gctgaaatga agtttattgc acgctatgtg gcaaccaatg cgaccgtaac ggctggtacg
      541 gcgaacgctg attcacaatt tactgtcgaa tataagaaat aatttatcaa gggccttttt
      601 attcagttgg ctttggccag ggagtcgtta tgtccagaaa aagttgttta atcaggctct
      661 ttaaaacaac agtttcatgt aagttattta ccgcgattat actcagcgtt tttttaggcc
      721 aaccagcatt aacttatgca ggcgttgtca ttggtggtac gcgggtggtt tacttaagta
      781 ataatgctga taaatctatt tctgtattta gcaaagaaga aaaaatacct tatctcatcc
      841 aggcttgggt agatccattt aataaagagg ataaaagcaa agcgccgttt accgttattc
      901 cgccagtatc acgattagaa ccttcgcaag aaaaaatatt acgcattatt cacaccaaag
      961 gtgtgtcatt gcctgatgat cgcgagtcag tcttctggct gaatattaaa aatattccgc
     1021 catcagcgag caacaaagcg acgaactcgc tggaaatagc agtaaaaacg cgtatcaagc
     1081 tgttctggcg accagcaaat atccgcctta ttcccgaaga tgctgcaccg aaagtgaaat
     1141 ggcgccggga agggcgaaat ctgatagcag agaatcccaa cccaattcat atcagtgtga
     1201 tggatgtcat cgttgatggc catgatgtgc ccctcaatat gatccgtcct tttgaaacgc
     1261 tcacgttacc tttacctgct aattctgcgg gaggccaaat gacctggcga tttattaatg
     1321 attacggcgc agtcagtgac ccgataaaaa tgacgctgta agcaagggat aaggataggc
     1381 gtattatgat gacgaccaga atagtggttg gcctcacggc agggacgtgt ctgattttct
     1441 cgcaaaacct gatggccgag gtcagtgtat tcaatccggc gcttctggaa atcgaccatc
     1501 aatccggagt cgatattcgc cagtttaatc gggcaaacct gatgccccca ggtgtttata
     1561 gcgttgatat ttttatcaac ggtaaaatgt ttgaacgtca ggatgtgaca tttgttcagg
     1621 ataatccaga tgctgatctg cacgcttgct ttattgccat taaaaaaaca ctgtcctcct
     1681 ttggcataaa agttgatgcg ctcaaatcgt tcaatgatgt ggatgagacg gtttgcctcg
     1741 atcctgctcc acgtattgaa ggctcatcct ggcagtttga cagtgataaa ttgcagctga
     1801 atatatccat tccccaaatc tacatggacg cgatggctta tgattacatc agccccacgc
     1861 gttgggatga ggggattaac gcgctcacca tcaactacga tttttctggt tcacatacac
     1921 tacgttcaga ttatggttca caagagacag ataccagtta tctcaatctg cgcaatggac
     1981 tgaatattgg accgtggcgg ctacgtaatt acagtacttt aaacaccagc gatggccgtg
     2041 cggaatacaa ctccattagt acctggatac agcgcgatat tgccgcgtta agaagccaga
     2101 ttatgattgg tgatacgtgg acggcgagcg atattttcga cagtacgcaa attcgcggcg
     2161 cgcgtttgta tactgataac gatatgctac ccgccagcca gaatggcttt gctcctgtgg
     2221 ttcgtgggat tgcaaagtcc aacgccaccg tcatcattcg gcagaatggc tacgtgattt
     2281 atcagtctgc cgttccacaa ggtgcttttg agatcaccga tctcaacacc gccagtacag
     2341 gtggcgattt ggacgtaacc atcaaagaag aagacggtag cgaacaacga ttcacccaac
     2401 cttatgcttc attggcgatt cttaaacgtg aaggtcagac agatgttgat gttagcgtgg
     2461 gtgaattgcg cgatgaagac ggatttacac cggacgtcct tcaggcgcaa atacttcatg
     2521 gtttttccca cgggatcact ttatatggag gtatgcaggc tgctgaaaat tatggttctg
     2581 cagctctggg tgtcggtaaa gatcttggcg ctttgggcgc aatttctttc gatgtgacac
     2641 atgctcgtgc gaattttagc catgatgata cagaaacggg tcagtcatat cgctttctct
     2701 attcaaaacg atttgacgac acagacacta gcttgcgcct ggttggctat cgttactcca
     2761 ccgagggcta ctataccctc aatgagtggg catcgcggcg caacagccct gaagactttt
     2821 gggaaacagg taaccgacgt agtcgcgtgg agggaacgct aacgcagtcg ttggggagag
     2881 attatggcaa tttatacctg acattaagcc ggcaacaata ctggcatacc gatgatgtcg
     2941 aacgattaat gcaatttggc tacagcagta gctggaagcg tctctcgtgg aacgtctcct
     3001 agagttattc caatactgcc agacagggga cggggaacaa ccatgccagt gataacacca
     3061 gtgagcagat ctacatgctc tctttatctg ttcctttatc gggctggtgg ggtaatagtt
     3121 acgccaccta ttctgtttcg caaaacgata attccggtag ctcacatcaa ctcggactca
     3181 gcggtacggc gctggaaaga aataaccttt catggaattt aatgcagtcc tataacagtc
     3241 atgatgatga ggttggcggt aatatgtccc tgacctatga tggctcttat ggcacggtga
     3301 acggcagcta taactacagc caaaattccc agaggctgaa ttatggtatc agagggggaa
     3361 ttctggcaca cagcgaaggg gtaacgttaa gtcaggagtt aggtgaaact attgctcttg
     3421 ttaaagcacc tggggccgcc gggttagaaa tagataatat gcgcggtgct gcgacggact
     3481 ggcgcggcta tacggtcaag acacagctaa acccttatga tgaaaatcgg gtagcaatca
     3541 gcgataacta tttctcgaag tcgaatatag aacttgataa taccgtcgtt acgatggttc
     3601 ccacgcgtgg tgcagtggtt aaagcggagt ttgtgactca tgtgggttat cgcgttctct
     3661 tcagagtgtt aaatgcaaat ggtaaaccgg taccttttgg agccattgct gcgatacaag
     3721 atgcaagttt ggcagattca ggaattgtcg gtgaccgtgg cgaactttat ctttctggtc
     3781 taccagaaaa aggacaggtt acgttatcct ggggagaaaa cgcctcaaca aaatgcatct
     3841 tcaattattc actttcgaca ccagaaagtg agagcggatt aattgaacag ggtgtgacat
     3901 gtcattaaaa ggatttttat gaacaagtat atcaaacagt ggtgctttgc tgtgtttatg
     3961 ctctcgttaa gtagtgtagc acttgcagct cctaaaggta tctgtacccc tgataatggc
     4021 gtatttcaca gtacgcttga tttctcggga tatcttatta ccgcgaacga aaataaggtt
     4081 ggaacgacat ttaacaccac tgtaactaat ggatcctcat atcctggacg ctgtcattgt
     4141 gatactggta acgtaggaga attcccctat atctattata cgtcaaaaat aaaccaggca
     4201 ttaacttatg ctggggttca ctctaatatt aattattatg atcttaatcc gaaccttgat
     4261 gttgggattg ctatagatat tcttggggtt gggtatgtga atgcaccttt tgaatatcat
     4321 gctaacaacc cgtctggtaa tacaaagtac aattgcaacc gcattgaacc tttaagtata
     4381 tctagtgggg ctaaagcgat agtatatttt tatatcaaga agacatttgc aggaaaattg
     4441 attattcctg aaacaaagat agtgacattg tatggaacaa ttagccgtga caccccggtg
     4501 gattactcac agccgatggc tgatgtttat attcggggcg atatttcagc tccgcaaagt
     4561 tgcgaaataa ataatttaca gccagtttat tttgatttta aagaaatacc tgctgcagat
     4621 ttttcatctg ttgttggaag tgcggtaaca acacataaaa ttactaaaac ggtcactatt
     4681 gagtgtgaaa acctggggat actgaatacc gatgatatca gtacgtcttt ttatgctacc
     4741 gaacccaata cggacaattc aatggtcgta acttcaaact ctaacgtggg gataaaaatt
     4801 tacgacaaaa ataataagga aatcaaagtg aacggtggtg agttgccaac agacatgggt
     4861 aaatcaacag tgtatggtga aaaatcaggt agtgtaactt tttcagctgc tcctgcgagc
     4921 cttactggag ctcgcccagc cccggggcaa tttactgcaa cagcgacgat aactgttgag
     4981 attgtgcgct aataggtctg acaacgattg ttcatcacat cgttgtcatc tcacccattt
     5041 ctttatcact tttaaatgca tcatgacaaa aagatgacat gtgggatttt catcttcaca
     5101 tttaaaatca gtaagttatt tggtaattcc tttctgtcat aaaaatgtca tattccttac
     5161 atataactgt cacctgtttg tcctattttg cttctcgtag ccaacaaaca atgctttatg
     5221 aatcctccca ggagacatta tgaaagttat gcgtaccacc gtcgcaactg ttgtcgccgc
     5281 gaccttatcg atgagcgctt tctctgtgtt tgcagaagca agcctgacag gtgcaggtgc
     5341 aaccttccct gcgccggtgt atgccaaatg ggctgacact taccagaaag aaaccggtaa
     5401 taaagttaac taccagggta tcggttcttc cggtggcgta aaacagatta tcgctaatac
     5461 cgttgatttt ggtgcctctg acgcgccgct gtctgacgaa aaactggctc aggaaggtct
     5521 gttccagttc ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc cagggctgaa
     5581 gtctggcgaa ctggtgctgg atggtaaaac cctcggcgac atctacctgg gcaaaatcaa
     5641 gaagtgggat gatgaagcca tcgccaaact gaatccgggt ctgaaactgc cttcacaaaa
     5701 cattgctgta gtacgccgcg cagatggctc cgggacttcc ttcgtcttca ccagctacct
     5761 ggcgaaagtg aacgaagagt ggaaaaacaa cgttggtact ggctctaccg taaaatggcc
     5821 gatcggtctg ggcggtaaag gtaacgacgg tatcgccgcg ttcgttcagc gtctgccggg
     5881 tgcaattggt tatgttgaat atgcttacgc gaagcagaac aacctggcgt acaccaaact
     5941 gatctccgct gatggtaaac cggttagtcc gaccgaagaa aacttcgcta atgcagcaaa
     6001 aggtgcagac tggagcaaaa ccttcgctca ggatctgacc aaccagaaag gcgaagatgc
     6061 atggcctatt acctctacca cgttcattct gatccacaaa gatcagaaga aaccagaaca
     6121 aggcacagaa gtgctgaaat tcttcgactg ggcgtacaaa accggggcta aacaggcgaa
     6181 cgacctggat tacgccagcc tgccggatag tgtagttgaa caggttcgcg ctgcgtggaa
     6241 gaccaatatt aaagacagta gcggtaagcc gctgtactaa taaaactcca ggccgggtac
     6301 ggtgttttac gccgcatccg gcattacaaa atgactttgt aaacgcgttt aactgaagag
     6361 taacttatgg ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg cgacataatt
     6421 ttcagcgtgc tggtaaaact ggcggcgctg attgtgctat tgatgttggg tggcattatt
     6481 gtctctctga tcatctcctc ctggccgagc attcagaaat ttggtctggc tttcctatgg
     6541 accaaagagt gggatgcacc gaacgatatc tacggggcgc tggtgccgat ctacggtacg
     6601 ttggtgactt cgtttatcgc gctgctgatc gccgtcccgg tgagtttcgg tatcgccctg
     6661 ttcctgactg agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc cattgagctg
     6721 ctggcagcca ttccaagtat cgtttacggc atgtggggcc tgtttatctt tgcgccgctg
     6781 ttcgccgttt actttcagga gccggtcggc aatatcatgt cgaatatccc gattgttggc
     6841 gcgctgttct ctggccccgc atttggtatc ggtatcctcg cggcaggcgt gatcctcgcc
     6901 atcatgatta ttccgtacat tgcggcggta atgcgtgatg tgttcgaaca aaccccggtg
     6961 atgatgaaag agtcggccta cggtattggc tgcaccacct gggaagttat ctggcgtatc
     7021 gttcttccgt tcaccaaaaa tggtgttatc ggcggcatca tgctggggct gggccgcgcg
     7081 ctcggtgaaa ccatggcggt gacctttatc atcggtaaca cctaccagct cgacagcgcc
     7141 tcgctgtata tgccgggcaa cagtatcacc tctgcgctgg cgaacgaatt tgcggaagcg
     7201 gaatccggtc tgcacgttgc cgcactgatg gaactgggcc tgatcctgtt tgtgattacc
     7261 ttcatcgtcc tcgccgcatc gaagtttatg attatgcgcc tggctaagaa tgagggggca
     7321 cgctaatggc tatggttgaa atgcaaacca ctgcggcgct ggctgaatct cgccgcaaaa
     7381 tgcaggcgcg tcgccgcctc aaaaaccgta ttgcgctgac gctctcgatg gcgacgatgg
     7441 ccttcggcct gttctggctg atctggattt taatgtccac catcacccgc ggtatcgacg
     7501 gtatgtcgct ggcgctgttc actgaaatga cgccgccgcc caatacggaa ggtggtggtc
     7561 tggcgaacgc cctggcgggt agcgggctgt taattttgtg ggccacggta ttcggtacgc
     7621 cgctgggcat tatggcgggg atttatctgg cggaatatgg tcgtaaatcc tggctggcag
     7681 aagtgattcg cttcattaac gacattctgc tctccgcgcc gtcgattgtg gttggtctgt
     7741 ttgtttacac catcgtggtg gcgcagatgg agcacttctc tggctgggcg ggtgtaattg
     7801 cgctggcgct gttgcaggtg ccgattgtta tccgcaccac cgagaacatg ctgaaactgg
     7861 tgccggacag cctgcgtgaa gcggcttatg cgctgggtac accgaagtgg aaaatgatct
     7921 ccgcgattac gctgaaagcg tcggtgtccg ggatcatgac cggtatcctg ctggcgattg
     7981 cccgtattgc cggtgaaacc gcgccgctgc tgtttaccgc gctctccaac cagttctgga
     8041 gcacggacat gatgcagccg atcgccaacc tgccggtgac gatctttaag tttgcgatga
     8101 gcccgtttgc agaatggcag caactggcct gggccggggt gttgattatt accctgtgcg
     8161 tactgctgct gaacattctg gcgcgcgttg tttttgcgaa gaataaacac ggttaacact
     8221 ttgccggatg cggcgtgaac gccttatccg gcctacggta agcctgattc gcgaagtgca
     8281 tcaggcacga tgaggaaaag attgcaatga gtatggttga aactgccccg agtaaaattc
     8341 aggttcgtaa tttgaacttc tactacggca aattccatgc cctgaaaaac atcaacctgg
     8401 atatcgctaa aaaccaggtt acggcgttta tcgggccgtc cggctgcggt aaatcgacgc
     8461 tgctgcgtac cttcaacaaa atgtttgaac tgtacccgga gcagcgtgcg gaaggtgaaa
     8521 ttctgcttga tggcgacaac atcctgacca actctcagga tatcgccctg ctgcgtgcga
     8581 aagtgggcat ggtgttccag aaaccgacgc cgttcccgat gtccatctac gacaacatcg
     8641 cctttggcgt tcgtctgttt gagaagctct cccgtgccga catggacgag cgcgtgcagt
     8701 gggcattgac caaagccgca ttgtggaacg aaaccaaaga taaattgcac cagagcggtt
     8761 actctctctc tggtggtcag caacagcgtc tgtgtattgc gcgtggtatc gccattcgcc
     8821 cggaagtgct gctgctcgac gaaccgtgtt cggcgctcga ccccatctct accgggcgta
     8881 ttgaagagct gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc acccacaaca
     8941 tgcagcaggc tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc gaattgattg
     9001 agttcagcaa cacggacgat ctgttcacca agccagcgaa gaaacaaaca gaagactaca
     9061 tcaccggtcg ttacggttga ttcaggagtg cgttatggac agtctcaatc ttaataaaca
     9121 tatttccggc cagttcaacg ccgaactgga aagtatccgc acgcaggtga tgaccatggg
     9181 cggcatggtg gagcagcagc tttctgatgc aatcaccgcg atgcataacc aggacagcga
     9241 tctggcgaag cgcgtcatcg aaggcgacaa gaacgtcaac atgatggaag tggcgatcga
     9301 tgaagcctgc gtgcgcatta tcgccaaacg tcagccgaca gcgagcgacc tgcgtctggt
     9361 tatggtgatc agtaaaacca ttgccgaact ggagcgtatt ggcgacgtgg cggacaaaat
     9421 ctgccgtact gcgctggaga aattctccca gcagcatcag ccgttgctgg taagtctgga
     9481 gtcgctgggc cgtcatacca tccagatgct gcacgacgtg ctggacgcgt tcgcgcggat
     9541 ggacattgac gaagcggtac gtatttatcg tgaagataaa aaagtcgatc aggaatacga
     9601 aggtattgtt cgtcaactga tgacctacat gatggaagat tcgcgcacca ttccgagcgt
     9661 acttaccgcg ctgttctgtg cgcgctctat cgaacgtatt ggcgaccgct gccagaatat
     9721 ttgtgagttt atcttctact acgtgaaggg gcaggatttc cgtcacgtcg gtggtgacga
     9781 actcgataaa ctgctggcag aaaaagatag cgataaataa ttcaccaaac aa
//
LOCUS       DAPHDX010000150         9818 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPHDX010000150 DAPHDX010000000
VERSION     DAPHDX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.152"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            129..344
                     /gene="bdm"
                     /locus_tag="RH058_003820"
     CDS             129..344
                     /gene="bdm"
                     /locus_tag="RH058_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV3139342.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            446..583
                     /gene="sra"
                     /locus_tag="RH058_003821"
     CDS             446..583
                     /gene="sra"
                     /locus_tag="RH058_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV3139343.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            740..2437
                     /gene="maeA"
                     /locus_tag="RH058_003822"
     CDS             740..2437
                     /gene="maeA"
                     /locus_tag="RH058_003822"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV3139344.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2571..3581
                     /gene="adhP"
                     /locus_tag="RH058_003823"
     CDS             2571..3581
                     /gene="adhP"
                     /locus_tag="RH058_003823"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV3139345.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3728..4012
                     /locus_tag="RH058_003824"
     CDS             3728..4012
                     /locus_tag="RH058_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV3139346.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4063..4716)
                     /gene="fdnI"
                     /locus_tag="RH058_003825"
     CDS             complement(4063..4716)
                     /gene="fdnI"
                     /locus_tag="RH058_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV3139347.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4709..5593)
                     /gene="fdnH"
                     /locus_tag="RH058_003826"
     CDS             complement(4709..5593)
                     /gene="fdnH"
                     /locus_tag="RH058_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV3139348.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5606..8653)
                     /gene="fdnG"
                     /locus_tag="RH058_003827"
     CDS             complement(5606..8653)
                     /gene="fdnG"
                     /locus_tag="RH058_003827"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8066..8068),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV3139349.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8885..9766
                     /gene="yddG"
                     /locus_tag="RH058_003828"
     CDS             8885..9766
                     /gene="yddG"
                     /locus_tag="RH058_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV3139350.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEELGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2372 a         2571 c         2492 g         2383 t
ORIGIN      
        1 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
       61 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
      121 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
      181 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
      241 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
      301 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
      361 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
      421 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
      481 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt
      541 ggtaaataat ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
      601 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctctata
      661 cttggggccg acgccctggc ggtaaagcaa agacgataaa agcgtgccag ggatggatat
      721 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc
      781 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat
      841 ggaagaacgc cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga
      901 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
      961 catctacctg cgtaacatcc aggacactaa cgaaaccctc ttctaccgtc tggtaaacaa
     1021 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
     1081 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accggcacaa
     1141 tatggacgat attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga
     1201 cggtgaacgt attctagggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
     1261 taaactgtcg ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt
     1321 gctggatgtc ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg
     1381 taatccgcgt atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt
     1441 gaaacaacgc tggccagacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat
     1501 gccgttactt aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac
     1561 tgcggcggta acagtcggca cactgatcgc agcaagccgc gcggcaggtg gtcagttaag
     1621 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
     1681 catctcccag acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat
     1741 ggtcgatcgc tttggcttgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa
     1801 actggtgcag aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgttgtcact
     1861 gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
     1921 gctgtttacg gaagagatca ttcgtgagat gcataaacac tgtccgcgtc cgatcgtgat
     1981 gccgctgtct aacccgacgt cacgcgtgga agccacaccg caggacatta tcgcctggac
     2041 cgaaggtaac gcgctggtcg ccactggcag cccgtttaat ccagtggtat ggaaagataa
     2101 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccgggcatcg gcctgggggt
     2161 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct
     2221 tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga
     2281 tattcagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg
     2341 cgtggcggtg aaaacgtctg ccgaagctct gcaacaggcc attgatgata atttctggca
     2401 agccgaatac cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct
     2461 accgggctat ttctctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa
     2521 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg
     2581 cagttgttac gaaggatcat cacgttgacg ttacggataa aacactgcgc tcactgaaac
     2641 atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta
     2701 agaatggcga ttttggtgac aaaactggcg taattctggg ccatgaaggc atcggtgtgg
     2761 tggcagaagt tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
     2821 ggttctacga aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc
     2881 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
     2941 tcgccgatta cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta
     3001 cctgtgcggg ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt ccagggcagt
     3061 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc tctgcaatac gcgaagaatg
     3121 tctttaacgc gaaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg
     3181 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
     3241 agaaaaccgg tggcgctcac gctgcggtgg taacagcagt tgctaaagct gcgtttaact
     3301 cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt
     3361 ctatgagcct ggatatccca cgccttgtgc tggatggcat tgaagtggtc ggttcgctgg
     3421 tcggcacgcg ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
     3481 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag
     3541 gcaaaatccg tggccgtatg gtgattgatt tccgccgtta agaggccttt gctgcgagtg
     3601 ccagtcctgg tgtcgcagca tcgcactctc tccgacatta aatggctaaa tggattattg
     3661 ttccttccta cgcaacaata atgatagtgt tcctgatgaa ggaacactac tgagagtaaa
     3721 gacactgatg aaaatggcca atcatccccg cccgggggac attattcagg aatcgctgga
     3781 cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgctc cctcaacggc
     3841 aagccgattg ctgaccggaa aagcagcttt gacaccagaa atggcgatta aactttccgt
     3901 ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gtctggcaga
     3961 ggcagaaaaa acggtggatg tgtcgcgact acgccgtctg gttacgcaat aaaaaaatgg
     4021 cgcttcttaa agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
     4081 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
     4141 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
     4201 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
     4261 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
     4321 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
     4381 cccggcgttg tacttaccga catccgccac tttatgctca ttgcctttca atacttcgac
     4441 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
     4501 aaacataaac atcagtgcga cgaaaatcgc aatgccgaaa aacgggtgca aaatgcgtcc
     4561 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
     4621 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
     4681 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
     4741 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
     4801 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
     4861 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
     4921 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg
     4981 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
     5041 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
     5101 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
     5161 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
     5221 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagccgggat
     5281 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgccg ttctgttcgg
     5341 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
     5401 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
     5461 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
     5521 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg
     5581 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
     5641 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
     5701 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
     5761 gaccatttac attcagtggt ttcagacgac gcgttaccac agccaccgcg cggataaagc
     5821 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
     5881 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaat gcgtgcttgg
     5941 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
     6001 ttttacccat ccgcagtgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
     6061 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
     6121 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
     6181 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc aggaatatcg ttacccgtcc
     6241 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
     6301 ccgccgaagc acggttgtac agcacgcgac ggttgagcgg ccacgcccag gcccatccca
     6361 gcgtattccc cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct
     6421 ctgtccagct accggtgtag atccagcaag aagatgcggt ggtaccgtca tcacgcagat
     6481 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
     6541 gatcttccag cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
     6601 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
     6661 ggtacagctc gcgcaggtga tggtagatac ccgccagaat ttcaccgtcg ttacgcgctt
     6721 cgcccggcgc atcctgacct ttccagtgcc actgcagcca gcgaccggag ttagcaatag
     6781 aaccatcttc ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
     6841 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggttgaggtt tcagtcacca
     6901 gcggatcgat aaccaccatg tacttcagct tgcttaggca gctcaccact ttgtttttgt
     6961 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
     7021 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
     7081 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
     7141 agaacttcgg atagttgctc cagtagttca cctgatcagc cagcgtcgct ttcggcgtgt
     7201 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
     7261 gcaggctggt agagagcaga cctaagtcag tcaggccctg aatgttggag tgaccacgca
     7321 atgcgttcac gccgccaccg gccataccca tgttaccgag cagtaactgg atcatcgcca
     7381 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
     7441 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
     7501 cttttggcgt accgcagatg ttttcaacga cgtccggcgt gtagcgggaa acgtgcgctt
     7561 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt
     7621 tttcatcgaa ctgatagttc caggacgatt tgtcgtactg gcgtttttca gcgtcataac
     7681 cgctgaacag gccatcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
     7741 aatgtttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
     7801 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
     7861 tacgcgtaaa acgaggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
     7921 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
     7981 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
     8041 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
     8101 cgagggagcg ggcaaatttc tgtgtcagca tcccggtttc gttgctggca ccggaggcac
     8161 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
     8221 agttagcgtc acggtcagct ttcatcagct ttgcaatacg ggagaatgct tcttcccagc
     8281 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
     8341 cgctgttgac gtaatccagc aaaccggccc cttttgggca cagcgcacca cggcttaccg
     8401 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
     8461 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
     8521 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
     8581 ccaatgccgc taccgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
     8641 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
     8701 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
     8761 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
     8821 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
     8881 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
     8941 gatggtagga ttgattcgcg gtgtcagcga ggagctcggc ccggtcggcg gcgcagctgc
     9001 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
     9061 gattccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
     9121 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
     9181 ctatctatgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
     9241 ctggctgatt gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg
     9301 tgataatggg ttacattatg atgaaatcat caataatatt accaccagcc cattgagtta
     9361 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
     9421 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
     9481 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
     9541 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatactgca
     9601 tggcaatgtc accataatgg cggtaggctc gtattttacg cccgtacttt cctcagcgct
     9661 tgcagccgtg ctgctcagcg ccccgctgtc gttctcattc tggcaaggcg cgctaatggt
     9721 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
     9781 ctacggcccg gcagttaatc agataaattc cgacaata
//
LOCUS       DAPHDX010000151         9792 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPHDX010000151 DAPHDX010000000
VERSION     DAPHDX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.153"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..118
                     /gene="ghoT"
                     /locus_tag="RH058_003829"
     CDS             <1..118
                     /gene="ghoT"
                     /locus_tag="RH058_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV3139351.1"
                     /translation="VIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
     gene            complement(237..1754)
                     /gene="lysS"
                     /locus_tag="RH058_003830"
     CDS             complement(237..1754)
                     /gene="lysS"
                     /locus_tag="RH058_003830"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV3139352.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1991..3448)
                     /gene="dtpC"
                     /locus_tag="RH058_003831"
     CDS             complement(1991..3448)
                     /gene="dtpC"
                     /locus_tag="RH058_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV3139353.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3507..5654)
                     /gene="cadA"
                     /locus_tag="RH058_003832"
     CDS             complement(3507..5654)
                     /gene="cadA"
                     /locus_tag="RH058_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV3139354.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5734..7068)
                     /gene="cadB"
                     /locus_tag="RH058_003833"
     CDS             complement(5734..7068)
                     /gene="cadB"
                     /locus_tag="RH058_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV3139355.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7434..8972)
                     /gene="cadC"
                     /locus_tag="RH058_003834"
     CDS             complement(7434..8972)
                     /gene="cadC"
                     /locus_tag="RH058_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV3139356.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPIKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9721..>9792)
                     /locus_tag="RH058_003835"
     CDS             complement(9721..>9792)
                     /locus_tag="RH058_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HDV3139357.1"
                     /translation="KRLELIDRINDIIARHFPSVLSA"
BASE COUNT         2735 a         2269 c         2237 g         2551 t
ORIGIN      
        1 tgtcattatc tggtttatct cacatgagaa aacacatatt cgtttactta gtgcattcct
       61 ggtcggaata acctggccaa tgagtctgcc tgtggcatta cttttttctc tcttttagga
      121 gctgtagttg agtggacccg ctggttatcg tgaaacgaag cgtctctcct caaaactgga
      181 tttgcggaac gacgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat
      241 ttctgtgggc gcatcgccgg gaagagaata acgtcgcgaa tagtatgact gtttgtaaac
      301 agcataatca ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc
      361 agcgcagtca cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc agctttagcg
      421 ttaacctgtt cctggaaacg ttcagcctga tcttctgcgt cgttcaattc gctaaaacca
      481 ttaccgattt cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca
      541 tcattacggc gcgccagcgg tgacacttct gccggatatt ccgtaataaa ggttggctga
      601 atcagatgtg cttctgccac ttcatcaaag atctccgtga caatacgtcc cagcccccag
      661 cttttctcta ccgtaattcc gatagattca gctaatgctt tagcagcatc aaaattatcc
      721 aggtcggcca tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt
      781 tcaaacggtt tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccaga
      841 acctcttgtg ccagggtgcg gaacagggac tctgtcagtt caatcaaatc gtggtaatcc
      901 gcatacgcca tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct
      961 tcattacgga agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt
     1021 ttcagataca gctccggcgc gatacgcagg tacatatcta aatccagagc gttatgatgg
     1081 gtaataaacg ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact
     1141 tccataaagc cgcgcgcgac catgaactgg cggatagcgg ccagaatttt tgaacggaca
     1201 acaaacgttt gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg
     1261 acttcctgat cctgcagacc atggaattta tctggtaaag gacgtagtgc ttttgtcagc
     1321 aggcgcaact cagtacagtg aatggaaagc tcaccggttt gcgtcttaaa cagcgtaccg
     1381 cgggcaccga taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct
     1441 ggcaggctat ctcttgcaac gtacagttga atacggccac cgacatcctg caacgttaca
     1501 aaggaggctt tccccatgat acgacgggtc atcattcggc cagcaaccga gacttcaatg
     1561 tttaaggatt ccagttcctg gttatccttc gcatcaaact cttcgtgcag ctggtcagag
     1621 gtatggtcgc ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt
     1681 ttttcgcggc gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt
     1741 tcttgttcag acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga
     1801 cagatcggta cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa
     1861 ctaacgctta cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact
     1921 tattaatctc gatttactag tgtgattaac tgaaagttaa tttattcata accaattgtt
     1981 tttacgacag ttaatcgttg ctctcctgta tcatattcgt cggcgtgcta aacagaaaac
     2041 gggtagcaaa ggccaatacg acgatgatcg cgacacaagc caacgtccac tctcccatct
     2101 gagaaaagaa tcgctggtaa gctgcaattg ccataccgct aatttgcgac tctgtcgtct
     2161 gctgtgccac gacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatcc
     2221 cggttaatac gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa
     2281 agagttcggc aaagcccatt agcgccagcc cggatatcat cacgcccatt gacgcttgac
     2341 cgtcagccgc tgcatgtcgg gcatcaaatg ccaacaacat aaagccacaa gccatcagca
     2401 gtaagccaaa ggcaaacttc agccagacgc gcaatgttga gttgccgcgg ctttctggcg
     2461 acgccagcca ggccagtaca accccagcga gcatcaccgc aattgcattc accgactgga
     2521 atagtgctgt aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca
     2581 agctgatggt actgccgccc tgttgtgcca gtacccagaa caacgtcccg acaaacatca
     2641 acaatacaat ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa
     2701 tgatttgtgc ggcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca
     2761 gcagcagggt aaaaaatact ggggctaaac agagcatcac cactaaccag ctccatactg
     2821 gtaaggcaaa tttcacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat
     2881 gacgatgccc gcttaagaaa atcaacaaac cgataaacat gccgccaccc gcaagggcaa
     2941 agccaacatg ccatccatac cactgagcag ccaggccgca ggcgattggg gcggcgatag
     3001 aaccgatatt gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct
     3061 cgtcgtagag ctcgccaagc aaacagctga tgtttgattt gaataaaccg tagccacaaa
     3121 taatgattgc cagcgccaga tacaggctaa aggttgaatt tgtatcaatg cccagcacca
     3181 catggccaag ggtcattaac agcgcgccgg caatcactgc agtgcggttg ccgagcaggc
     3241 ggtcggcaag ccagccgccg agaatagggg taacgtaaac cagagaagca tatgcgctga
     3301 acaggctgat ggcatggtta tcatcaaaac caagctgatg ggtgagatag agaatgagta
     3361 aggcacgcat gccgtaaaaa ctgaagtact cccagatttg gatcgccacg atatagtata
     3421 tcgcgcgcgg ctgtgagggt gttttcatgt gttctcctta tgagcaaaaa agggaagtgg
     3481 caagccactt cccttgtacg agctaattat tttttgcttt cttctttcaa taccttaacg
     3541 gtatagcggc catcagcctg acggtatgca ccgtgaatat cggtttcaaa gcccggatag
     3601 tgagcgccga tttcacacag catctgcagg aactccagaa ccggacggct ttcttcggtg
     3661 atcatttcac ccggcattac cagaggaact cccggcggat atggaaggat catattggcg
     3721 ttaatacgac ctaccatttc gtcgaggtaa acttcttcgg tcataccgtg cagctctttc
     3781 tggaacgcag catacggagt cattaccatc gtcggcagca cttcaaatgc gcgatacatc
     3841 agatccggca gattgtggtg aacaatcagt ttgtggatat tctgagccag ttcctgaata
     3901 cgcatgtttt catagaattc aggatcttca cgatacagag acggcagcat gtttttcaca
     3961 cgcaggttca ggtcgaacgc acgtttgaag tcagtcagag cacgcagcag gctcagtgct
     4021 ttggtcttat cgataccgat gctgaacagg aacagcaggt tatacggacc ggttttctca
     4081 acaacgatgc catgttcgtc gaggtatttc gccacgatgc tggccggaat accaaagtcg
     4141 ctcatggtgc cgtctttttc catccccgga gtcagcaggg tgactttgat cgggtcaaga
     4201 tacatgtgct cgttatcgat gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc
     4261 cagcattcag tcgtatcgat atgatccggc tgccatacat caaagaacca gccatcagat
     4321 tccgttctca gacgtttgat ctctttacgg aatttgatcg cacgttcaat ggaaccgttg
     4381 atcagacgtt tacctgcatt gcctttcatc atcgccgcag cggtttcagt ggacgccacg
     4441 ataccgtagt gcggagaagt ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg
     4501 tttacgtcac ctttaacgtg gatcatggaa gcctgagaga acgccgccag cagtttgtga
     4561 gtggactggg tttcgtaaat cactttccct tctacacggc caccgctcat accgcattta
     4621 ccttcgtaaa tcggtgagaa gttggtgtaa ggcacccacg cggagtcaaa gtggatggat
     4681 ttcacatcca gtgttttctt gatgaagtcg gtgttgtaca gcagaccatc ataggtagag
     4741 ttggtaatta cagcatgtac cggccaggtt gcgtttggtg tttctttcac gcgcttagca
     4801 atggtagcgt gctggaattc actctgtggg ataccaccaa gaataccgta agcgttacgg
     4861 gtcgggcgga aatagattgg cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg
     4921 tggcagttac ggtcaatcag aatggtgctg cctgccggag cagagtacat gccaacaatt
     4981 ttgttcgcag tggaagtacc gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga
     5041 gcgatatact gttctgcttc tttgtgtgga ccactgtgat ccagcagaga acccagttca
     5101 gatactgaaa tggaaatatc agatttcatg gtattcggac caaagaaatc atagaacagg
     5161 ctacctaccg ggcttttctg gaatgcagta ccgcccatgt gaccaggagt acagaaagta
     5221 tatttacctt cacgaacata tttaaacagt gctttagtca gcggaggcag aatagtgttg
     5281 atatattcgt cagtggtctg cttgatctta ttagcaatat cttcagcagc acccagcgca
     5341 tattcaaaga agctaatctg taaacgcagg tcattcaggc ttacatcgag agtggaatac
     5401 gtattagcga acgcgtacaa cggcaggttc tcgttcattt tgctaatttc ttcgcacagc
     5461 tcgagattat atttatccca gtcaaaaata acgccgcaca gacgcgcatt gttttcgatc
     5521 agttttaata agtcgtcacg gtcgttcggg taaacaatct ggaagttcag acgttcaagc
     5581 gcgcgatgaa gttcacggat gggttcttct ttaaaataaa cccccatgtg attcaatatt
     5641 gcaataacgt tcatagtcat atctccaggt aaaaaaggcc cctcccaaca catgggacaa
     5701 aatgaaagga ggagcctcgg aaaatacttt taattaatgt gcgttagacg cggtgtggtt
     5761 atccattgag tggctctggc gctcgtgcat tttgcgagcg tagaacatca ggataatcag
     5821 gctgacgatg aaggtacctg ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca
     5881 gaacacgcaa cccagtacag agcagatcag gctgacaaag ttgcggatgt taacgccttc
     5941 aaaacgaatc aggtcaacgc aagagtagaa gtacggcagc atagtcagca gtactgcgat
     6001 accggtcagt tcaccgaaca ggtcagatgc tttaccaccg gcagagttca tcagagtgat
     6061 gaggatcatc agggcagtca ttttcactgc agccagcagc agaccttttt tcggaatacc
     6121 gttgctgtcg acttcaccat aaactttcgg gaagttaccg tcgttagcgg cacgtacacc
     6181 tgcctggcct accaacatca tccaggagcc cagagaagtc aggcacgcaa aggcggtgaa
     6241 tgcagaaacc agcggcgcag cccagttacc gaggatggtt gaagcactga ttgcaaacgg
     6301 agcaccggaa gctgccatta cagaagatgg atacataccg gaaagcacct gagtcgcagc
     6361 gatgtaaaca atacctgcta aaccagtacc cagcatggtt gccagcggaa cggtacgttt
     6421 cgggttttta accataccag tacttactgc tgcggattca acacccacga aggcccacag
     6481 gcagagcaga atacttttaa tgatcgcatg accatcagtg gtatccgcag tattccagtt
     6541 agctgcataa gttgccgcat caaaccaatg ccagccaaca atagcagtca tcaccacagg
     6601 aataagaacc agcaccagac caatagtggt taaacggctt acccaagtac cgccgagcat
     6661 atttacaaag gtaaataccc agacgatagc aatacaggcg atacccgccg gaacaggatc
     6721 atttaatact gggaagaagg tggaaagata agataccgcg gtaataccaa tcgccaggtt
     6781 accaatccag ttagcatggt aataaagaac acctgtctga aaaccaaatg caggggaaat
     6841 ttctccggca taagcaattg ggccaccttg ttgcgggttt tttgttgcca gtcgggcata
     6901 tacatacgcc agcgacattg caccaataat agagataatc caaccccaga tagcaatacc
     6961 accgatactt gctaggttcg caggtaataa tgcaataccg ctccccatca tattaccggc
     7021 aacaacaccg gtacaggcaa atagcccgat cttcttggca gaactcatgc tcttctccta
     7081 atttcatttt tgaatttgga gtccgggtca tgatgtataa ctatttcctg accagaccaa
     7141 actggcgcta agaatactca cgaaaaaagg attaatccta aagattaggt gaaataacac
     7201 aaaagtttct gtaagtgaga acttgaggtt ttttattaac acatcaggat cgcaagttga
     7261 tatcatgaaa agataaacat tcaatgttta caatggattg cgggatattc tctggttaaa
     7321 tttatgtaat aaaaattatg cggcaaataa attgccgcaa catattatac caacagaaac
     7381 atacaaaaac tcaacaacaa atattcccga gcataaatca acccgggagt tacttattct
     7441 gaagcgagaa atttgtcgag ataaggtaca acataaggaa cagaagtctg gaatatacca
     7501 ttttcaatcc agtaaagggt gtttgcccct gggcgtaaat taaaggcggt gagatatgca
     7561 tcagccgctt cccggttcat ccccttcatt tcataaacct tgccaagcaa cacataattt
     7621 agccaggaca tttcaagatc aatgccagta tttatcgcct ggtaagactc atctgtttta
     7681 ccttttacca gagcactgac cgcttttatt tgatatataa tggataggtt attcaattcc
     7741 ggtagtgtaa caatgttgtc tatttctgtg ttcagtgctg ctaattgttt ttcatctaaa
     7801 gggtgttgag aatggcgcac gatatcaact aatgcttttt ctgctctcgc gtaggtaaat
     7861 tctggggatg attgaacaat ctcacctaat aattcactgg cacggttcaa tgatttatca
     7921 tcgccatgca gtaaataatc atgtgcctga taaaaattag ttaataacgc accacgatga
     7981 ggcaaaattt gctggagcgt ctcctgcatt cgttgtggcc acggttggtt taatgctttt
     8041 gataaactct ccagtaaatc attttgaatc gccagctgat taccgttagt gatgacataa
     8101 cgtttatcca gcatggttga accatctgca ttgtctacca attttatcga cataaagcat
     8161 tgttgagcac ggtattggcg ctgattaaca aaggcaatag ataatgtttt accggaactg
     8221 ctcggttcat caatgttgta gttgattttg tcatgcacca taaaggtgga gaaggtgtta
     8281 agtgatgtcg ccaccaaatc acccacgcct atcgcgtaag agagctgata cggggaactc
     8341 cagctgttac aacttttatt taccatatta atgtcaatat cgcgtggatt gagcaaaata
     8401 cgcgatttgc tcataggaag acgtgtatca agagttgaaa acgctaccag tgctacacag
     8461 atacctaacg acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg tttgctttta
     8521 attggggatt gttcaggtgg cgttgcggtg ttttgaatgt taagactgtg ggagggagaa
     8581 tctgtggcag gaaccgcctc tggtataggg ggaggcgaag atagcattat ttcctctccc
     8641 tcttcttcgc tgtaccagat aaccggcacc attaatttat agccgcgctt tggtacagta
     8701 gcgatataga caggactatc ttcatcatta tcttttaatg acttacgtag ttctgagata
     8761 ctctgcgtca caacatgatt ggtgacaata cttctcttcc agacattatc gataagttca
     8821 tccctgctaa gtacttcgcc actgtgttga gcaaagaaaa ccagaagatc gattaatctc
     8881 ggctcaaggg taagttgacg cccattgcgg ctaatttggt ttatggacgg agtaacaagc
     8941 cattcgccaa cgcgaactac aggttgttgc ataatagaaa ctcattcgaa aagggaatga
     9001 tgcaatgata attgccacaa cctattttta ccatctatag atgggtttat ttacatatta
     9061 ttggtgaatg taagacgtta tttttaccag ccataaactt cttgattaca tagtattacg
     9121 aaaggatttt actgagaacc agaagtaata ttccttacca tcaaaattca tcatctttgc
     9181 caaagaaaaa tgttcagaaa ataatccatg gaaaattgtc cggagcgctt actattttaa
     9241 tggattgtta gtctttgcat gagcaagcgg actgatacat ttctctttgt tctcattcag
     9301 aaaatctcat cagtcggcgt tcagaccggt gttgtgcttt atggatgcgt tacgctcctg
     9361 atgacgtcat ttgacgttca acaacatcac ggggccgcac gacatttcac gtcagttagt
     9421 gctatcgctc aggaacaaat tttcccgaat tgggatatgc ccgcaaattg ctggtgatga
     9481 ggggagaatc tggttgagtt cgggagaatt gatttggagg cagaacgctt aaatcgtggc
     9541 gtcctgaaac gaaaaacgga cctccgtgga ggtccgttta tatgaatttg gtgccggact
     9601 cggaatcgaa gaatgcttct atcatgttga tattagttta ttttgtttgg tttggtgaaa
     9661 tatgtaccct cttttgtacc cgcagtccct tttgatatcg aaagggaatc aaactcgggg
     9721 tcaggctgag agcacagaag gaaagtgcct ggctattata tcgttaattc tgtcaatcag
     9781 ctccagcctt tt
//
LOCUS       DAPHDX010000152         9646 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPHDX010000152 DAPHDX010000000
VERSION     DAPHDX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.154"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            60..1046
                     /gene="iolG"
                     /locus_tag="RH058_003836"
     CDS             60..1046
                     /gene="iolG"
                     /locus_tag="RH058_003836"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDV3139358.1"
                     /translation="MFNIALLGAGRIGQVHAANIASHSATTLWSVVDPNQEFATRLAT
                     QYQARQQSLDEAMTDPNVHAVLIASATDTHADLIEMAARYGKAIFCEKPVHLDLARVR
                     DCLKVVKEYDVPLFIGFNRRFDPQFRRVKTDTQAGRIGKPESLLIISRDPSPPPAEYV
                     RVSGGMFRDMTIHDFDMARFIMGEEPVSVYAQGSNLVDPAIGGAGDIDTAFIVLKYAS
                     GAMATIVNSRRSSYGYDQRLELHGSEGLLCAGNILENQVQHYGKQGCTSALPEHFFLQ
                     RYKSAYAAEWEHFVAVLRGEAVPDCSGDDGERALYLADKALESLRCQREIVL"
     gene            1078..2007
                     /locus_tag="RH058_003837"
     CDS             1078..2007
                     /locus_tag="RH058_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV3139359.1"
                     /translation="MKKLILAALIAMTSGAAIAENEQIVFSTPNLAMPFEVHMQRTAV
                     KAAKEMGVKLQVLDGQGSSPKQVADLENAITRGAQGFIVSPNDVNAVSSAVDEIQDTK
                     LPVVTLDRSVDSQKKVPHFGANNYKGGQAIGDFVKTKFPNGAEIILLTGQPGSSSNIE
                     RTKGIRDSLKAGGDMYKIVADQTGNWMRSEGMRIVESVLPSLPKRPQVILSANDDMAL
                     GAIEALQSQGVKPGEILVTGFDAVPEALARVRDGWLAVTADQRPGFAVTTAMSQLVAN
                     VREKKTITGADYPPTLITKENLQQAERIAEAGN"
     gene            2059..3606
                     /locus_tag="RH058_003838"
     CDS             2059..3606
                     /locus_tag="RH058_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3139360.1"
                     /translation="MSQPLLKVTDLAKSFSGVWALSSAQLTVGTGEIHALLGENGAGK
                     STLLKALAGAQPQTRGEIWFNGETLPVDDSPVERQNKGIITIYQEFNLLPNMTVAENM
                     FLGREPRKRNLIVDEKAVNQEAQVILDYLQLNVAPTTPVARLSVAQQQMVEIARALTL
                     NARLIIMDEPSAALSDSEVESLHRVVRELKNRGVSIIYVTHRLHEVFQLCDRFTVFQD
                     GRFTGTGAVTETNVEQLIRLMVGRDVAFNRRPASETHHEDKPVRLSVKGLSREKPPLD
                     PHGIALHDISFHVHAGEVLGIAGLVGAGRTEVARCLFGADAFTTGSFELDGVPYQPRD
                     PLYALEQGLALVPEDRKKEGAVLGLSIRDNLSLSCLSSLLQWRWFVNTRKEDDLIESY
                     RQALQIKMVNSTQEVRKLSGGNQQKVILARCMALNPRVLIVDEPTRGIDVGTKSEVHQ
                     VLFDMAKKGVAVIVISSDLPEVMAVSDRIITLSEGRLTGEIHGDDANEERLMTMMAIN
                     HNALNAA"
     gene            3618..4646
                     /locus_tag="RH058_003839"
     CDS             3618..4646
                     /locus_tag="RH058_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3139361.1"
                     /translation="MTQMIPKTQVPVKRAGRIDPIAFFERFGVLIFMILLLIFFQSQN
                     SNFFSERNIFNILTEVSIYGIMAVGMTFVILTAGIDLSVGSILAVCAMTAAYVIKGDN
                     FTTVDPNAWGGMSWLIGLGICLTMGTAIGFLHGLGVTRLRLPPFIVTLGGMTIWRGLT
                     LVINDGAPIAGFDQGYRWWGRGELLGISIPIWIFAIVAIGGYLALHKTRWGRFVYAIG
                     GNPEAARLAGVNVKRVLVSVYVLIGCLAGLAGFILSARLGSAEAVAGISFELRVIASV
                     VIGGTSLMGGYGRIGGTIIGSIIMGILINGLVLMNVSAYYQQIITGLIIVLAVAFDTY
                     AKSRRGAL"
     gene            4655..5788
                     /locus_tag="RH058_003840"
     CDS             4655..5788
                     /locus_tag="RH058_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3139362.1"
                     /translation="MKEVRIGLIGTGYIGKAHAIAYAQAPTVFNLRGKLVREMVAEVN
                     PALAAERAQAFGFNRSTGDWRVLVADPAIDVVDICSPNHLHKEMALEAIRHGKHVYSE
                     KPLALNAHDAREMVDAAKRAGVKTLVGFNYMKNPTAQLAKEIIARGEIGEVIHFYGTH
                     NEDYMADPLSPIHWHCFKETAGLGALGDLAAHIVNMAQYLVGEIEQVCGDLKIVVPAR
                     PAKAGSSEMVTVENEDQAHAMVRFAGGAQGVIETSRVACGRKMGLSYVITGTKGAISF
                     TQERMAEIKLYLHDDPVNRQGFQTLLVGPAHPDYGAFCMGAGHGIGFNDQKTVEVRDL
                     VDGIATDAPMWPDFEEGWKVSRVLDAIALSHQQGLWLNVNDIV"
     gene            5801..7705
                     /gene="iolC"
                     /locus_tag="RH058_003841"
     CDS             5801..7705
                     /gene="iolC"
                     /locus_tag="RH058_003841"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDV3139363.1"
                     /translation="MEKQFDVICMGRVAVDLYSQQIGARLEDVSSFAKYLGGSSGNVA
                     YGTARQGLRSSMLARVGDEHMGRFLREELNQVGCDTSHLITDKERLTALVLLGIKDRD
                     TFPLIFYRDNCADMAITASDVDESYIASARCIAITGTHLSHPQTREAVLTALNYARRH
                     GVRTVLDIDYRPVLWGLTSLGDGETRFIVAEQVTRELQEVLHLFDVVVGTEEEFHIAG
                     GSTDTLQALAQVRHVSKATLVCKRGALGCSVYTDAIPPRLDDGLTVTGVRVEVLNVLG
                     AGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPSKIELDDYLSRAAL
                     VPRPDIDPRLNHLHRVTTRRREWPELCVMAFDHRSQLEDMALQCGASLKRIPALKQLI
                     LQASREAASSAGLEGKAGLLCDGTFGQDALNAITGEGWWIGRPIELPGSRPLEMEHGN
                     IGTQLISWPQEHVVKCLVFFHPEDAHGLRLEQEQKIAEVYHACCQSGHELLLEVILPA
                     TMPRSDELYLRAISRFYNLGIYPDWWKLPPLSAEGWTALSEIIARRDPHCRGVVILGL
                     DAPAEQLRADFKAAAGQALVKGFAVGRTLFGDASRAWLKHDIDDAQLVARIRDNYLQL
                     IAWWRERGHA"
     gene            7717..8607
                     /gene="iolE"
                     /locus_tag="RH058_003842"
     CDS             7717..8607
                     /gene="iolE"
                     /locus_tag="RH058_003842"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010433880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDV3139364.1"
                     /translation="MSVQLGINPLTWTNDDLPSLGAETSLETCLSEGKEAGFAGFELG
                     NKFPREARLLGPILQRHDLQLVSGWYSGRLLERSVEDEIAAVQSHLTLLRELGAKVLV
                     FAEVSGCIHGEQQTPVHLRPRFPQECWKEYGEKLTVFARYTQQQGVQIAYHHHMGTVI
                     ESAQDVDNLMLHTGEEVGLLLDTGHLTFAGADPLAVAQRWASRINHVHCKDVRADVLA
                     DVKNRKTSFLDAVLSGVFTVPGDGCVDYPPIMQLLKTQDYHGWLVVEAEQDPAIAHPL
                     TYARLGYNNLSRLARDAGLI"
     gene            8617..9438
                     /gene="iolB"
                     /locus_tag="RH058_003843"
     CDS             8617..9438
                     /gene="iolB"
                     /locus_tag="RH058_003843"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDV3139365.1"
                     /translation="MSRLLSRWQQPNAEGRTQSVTPESAGWGYVGFEAYELEEGQQLT
                     LPAVSEERCLVLVAGRAFISTPSAQFTDIGERMSPFERIKPWAVYVTPQEAVQVKALT
                     KLELAVCTAPGKGTYPTRLIAPQDIDGEARGNGHNQRYVHNILPEDKPADSLLVVEVW
                     TNEGCTSSYPSHKHDTNNPPQESYLEETYYHRLNPEQGFCMQRVYTDDRTLDECMAVY
                     NRDVVMVPKGYHPVATMAGYDSYYLNVMAGPVRKWIFTWEKEHSWINTEYSAISL"
BASE COUNT         2038 a         2607 c         2958 g         2043 t
ORIGIN      
        1 ataaatgttt tatttgtagt gtcgttagtt caccgcattc atcaacaccg ggagccgtta
       61 tgtttaacat tgctttactg ggcgctggcc gaattggcca ggtacatgca gctaacatcg
      121 ccagccatag tgcgaccacg ctctggtctg tggtagaccc gaatcaggag ttcgccacgc
      181 gtctcgcgac acagtatcag gcgcgccagc agagcctgga tgaggcgatg accgatccga
      241 acgttcatgc cgtattgatc gcctccgcga ccgacaccca cgcggatctg attgaaatgg
      301 ccgcccgtta cggcaaagcc attttctgtg aaaaaccggt acaccttgat cttgcccgcg
      361 tacgcgactg cctgaaagtg gtcaaagagt atgacgtacc gctgtttatc ggttttaacc
      421 gccgctttga cccacagttc cgtcgcgtga aaaccgacac tcaggccggg cgtatcggca
      481 aaccggaatc actgctgatc atctcccgcg atccgtctcc accgcctgcg gagtacgtgc
      541 gtgtttccgg cggcatgttc cgtgatatga ccatccacga ctttgacatg gcacgcttca
      601 tcatgggtga agagccggtg tcggtgtatg cacagggcag caatctggtg gatccggcga
      661 ttggcggagc gggagatatc gacaccgcat ttatcgttct gaaatatgcc tccggcgcga
      721 tggcgaccat cgtcaacagt cgccgctctt catacggcta cgaccagcgc ctggagctac
      781 acggttccga agggctgctg tgcgcgggca atatccttga aaaccaggtg cagcactacg
      841 gcaaacaggg ttgcaccagc gcgctgccgg aacacttctt cctgcaacgc tacaaatccg
      901 cttacgcagc ggaatgggag cactttgttg cggttctgcg cggcgaagcg gtgcctgatt
      961 gcagcggcga tgatggtgaa cgtgcgctgt acctcgcgga taaagcgctg gaatcactgc
     1021 gttgccagcg tgagatcgtc ctctaaacac aacaacccta cacagagaga cataataatg
     1081 aaaaaactga tcttagccgc cctaatcgcc atgacatccg gggccgccat agccgaaaac
     1141 gagcaaattg ttttcagtac gccaaacctg gccatgccgt ttgaagttca catgcagcgc
     1201 acggcggtga aagccgcaaa agagatgggg gtgaaacttc aggtactgga cggtcagggc
     1261 agctcaccga agcaggtggc tgacctggaa aacgccatca cccgtggtgc gcagggcttt
     1321 atcgtctccc cgaacgatgt gaacgcggtc tccagtgcgg tagatgaaat tcaggatacg
     1381 aagctgccgg tcgttacgct tgaccgctct gtcgacagcc agaaaaaagt gccgcacttt
     1441 ggtgccaaca actacaaggg cggccaggcg attggcgact tcgtcaaaac caaattcccc
     1501 aacggcgcgg agatcattct gttgaccggt caaccgggtt cctcctccaa cattgaacgc
     1561 accaaaggga tccgtgacag cctgaaggct ggcggggata tgtacaagat cgtcgccgat
     1621 cagaccggca actggatgcg ctcggaaggg atgcgcattg tcgaaagcgt tttgccgtcg
     1681 ctgccaaaac gtcctcaggt gatcctctct gccaacgatg atatggcgct gggggcgatt
     1741 gaagcgctgc agagtcaggg tgtgaaaccg ggtgaaatcc tggtcaccgg ttttgatgcg
     1801 gtaccggaag cactggcccg cgtgcgcgac ggctggctgg cggttacggc ggatcagcgt
     1861 cccggctttg cggtgaccac ggcgatgagc caactggtgg ctaacgtgcg tgagaaaaaa
     1921 actatcaccg gggcggacta tccgccgacg ctgatcacca aagagaacct gcaacaggct
     1981 gagcgtattg ctgaggccgg taactgattt gtctggagcc gccgtacggg cggctcccca
     2041 taaataagga gcaggctgat gtcgcaaccc ttactgaaag taaccgacct ggcaaaaagc
     2101 ttttccggcg tatgggcgct gagcagcgcc cagctcaccg tcggcacggg tgaaattcac
     2161 gctttgctgg gcgaaaacgg cgctgggaaa tccacgctgt tgaaggcact cgcgggcgca
     2221 cagccacaaa cgcgcgggga gatctggttc aatggcgaaa cgctgccggt ggacgactcc
     2281 ccggtagaac ggcagaacaa gggcattatc accatctatc aggaattcaa cctgctgccc
     2341 aatatgaccg ttgcggaaaa catgtttctc ggacgcgaac cgcgtaaacg taacctgatc
     2401 gtcgacgaaa aagcggtcaa tcaggaagcg caggtgattc tcgattatct gcaactgaat
     2461 gtcgcaccca ccacgccagt cgcccgtctg agcgtggccc agcagcagat ggtggagatt
     2521 gcccgggcgc tgaccctcaa tgccaggctg atcatcatgg atgaaccctc cgccgccctg
     2581 agtgacagcg aggtggaaag cctgcaccgc gtggtgcggg agcttaaaaa ccgcggcgtg
     2641 agcattattt acgttactca ccgtctgcat gaagttttcc agctttgcga tcgtttcacc
     2701 gttttccagg atggccgttt caccggcacc ggcgcagtga cggagacgaa cgtagagcag
     2761 ttgatccgcc tgatggtagg gcgtgatgtg gcctttaacc gccgccctgc cagcgaaacc
     2821 caccatgaag acaaacctgt ccgtttgtcg gtgaaggggt taagccggga aaaaccgccg
     2881 ctggatccac acggcatcgc cctgcatgac atcagttttc acgtccatgc tggggaagtt
     2941 ctgggtatcg ctgggctggt gggggctggt cgtactgagg tagcgcgctg cctgtttggc
     3001 gccgacgcct ttaccaccgg cagctttgaa ctggacggcg tgccgtacca gccgcgtgac
     3061 ccactttatg cgctggagca agggctggcg ctggtgccgg aagatcgcaa aaaagagggg
     3121 gcggtgctgg gtctgtcgat tcgcgacaac ctgtcgctct cctgcctctc ctcgctgttg
     3181 cagtggcgct ggtttgtgaa tacccgcaaa gaggacgacc tgattgagtc ctaccgccag
     3241 gcgctgcaga tcaaaatggt caacagcacg caagaggtgc gtaagctctc tggcggcaac
     3301 cagcagaaag ttatcctcgc gcgctgcatg gcgctcaatc cacgggtgtt gattgtcgat
     3361 gagccgacgc gtggcatcga cgttggaacc aaatcggaag tgcaccaggt gctgtttgat
     3421 atggcgaaaa agggcgtggc ggtgatcgtg atctcctccg atctgcctga agtgatggcg
     3481 gtgtctgacc ggatcatcac ccttagtgaa ggccggttaa ctggcgagat ccacggtgac
     3541 gacgctaacg aagaacggct gatgacgatg atggctatca accacaacgc cctgaacgcg
     3601 gcataacgga gtttctcatg acgcagatga ttcctaaaac gcaggtcccg gtaaaacgcg
     3661 ccggacgcat cgatcccatt gccttcttcg agcgcttcgg ggtactgatt ttcatgatcc
     3721 tgctgctgat cttctttcag tcgcagaaca gtaatttttt ctctgaacgc aatattttca
     3781 acatcctgac cgaagtctcc atctacggaa tcatggccgt ggggatgacc ttcgtcattc
     3841 tgaccgctgg gatcgacctc tccgtgggct ctattctggc agtgtgcgcc atgacagcgg
     3901 cgtacgtgat caaaggcgac aatttcacca cagtcgatcc gaacgcttgg ggcggtatga
     3961 gctggctgat tgggctgggg atttgtctga ccatgggtac agcgattggc ttcctgcacg
     4021 gtctgggcgt cacccgcctg cgcctaccac cgttcatcgt cacccttggc gggatgacca
     4081 tctggcgtgg tctgacgctc gtgattaacg acggtgcgcc gattgccggc ttcgatcagg
     4141 gttatcgctg gtggggacga ggggaattgc tcggcatctc cattccgatc tggatcttcg
     4201 ccatcgttgc cattggtggt tatctggcac tgcataaaac ccgttggggg cgcttcgtct
     4261 atgcaatcgg tggcaacccg gaggcggcac gtctggctgg ggtaaacgtg aagcgtgtgc
     4321 tggttagcgt ctatgtgctg attggctgtc tggcggggct ggcgggcttc atcctgagcg
     4381 ctcgtctggg gagcgctgag gcggttgccg ggatctcctt tgagctgcgt gttattgcct
     4441 cggtggttat tggcggcacg tcgttgatgg ggggatatgg gcgtatcggc ggcactatca
     4501 tcggctccat catcatgggc attctgatta atggcctggt actgatgaac gtctcggcct
     4561 actaccagca aattatcacc ggattaatta tcgttctggc ggtggcattc gacacctatg
     4621 ccaagagccg tcgcggcgca ctgtgaggat cacgatgaaa gaggttcgta ttggattgat
     4681 tggcaccggg tatattggca aggcgcatgc cattgcctat gcccaggcac cgacggtgtt
     4741 caatctgcgc ggcaaactgg tgcgcgagat ggtggctgag gtgaatccgg cgctggcggc
     4801 agaacgcgcg caggcgtttg gcttcaatcg ctcaaccgga gactggcggg tgctcgtggc
     4861 cgacccggcc attgatgtgg tggatatttg ctcccctaac catcttcaca aagagatggc
     4921 gctggaggcg atccgccacg gcaagcacgt ctattcggaa aagccgctgg cactgaatgc
     4981 ccacgacgcg cgtgagatgg tcgacgctgc gaagcgggca ggggtgaaaa cgctggtggg
     5041 tttcaattac atgaaaaacc cgacggcaca gttggcgaaa gagattattg cgcgcggcga
     5101 aattggtgag gtgatccact tctacggaac ccacaacgaa gactatatgg ccgacccgct
     5161 ttcccctatt cactggcact gttttaaaga gaccgccgga ctgggggctc tgggtgacct
     5221 ggcggcacat atcgtcaata tggcccagta tctggtggga gagattgagc aggtctgtgg
     5281 cgatctgaaa attgtggtgc cggcacgtcc ggcaaaggcc ggatcgtcgg aaatggtcac
     5341 cgtggaaaat gaagatcagg cccacgcgat ggtgcgcttt gcgggcgggg cgcagggcgt
     5401 cattgaaact tcccgcgtcg cctgcggccg caagatgggg ctgtcatacg tcattactgg
     5461 taccaaagga gcgatcagtt ttacccaaga gcggatggca gagataaagc tctatctgca
     5521 tgatgatccg gtgaaccgtc aggggttcca gacactgttg gtcggtccgg cacacccgga
     5581 ttacggcgcg ttttgcatgg gcgcaggtca tggtatcggc tttaacgatc aaaaaacggt
     5641 ggaagtgcgt gatctggtag acggcattgc caccgacgca ccgatgtggc cggacttcga
     5701 agagggctgg aaggtctcgc gcgtgctgga tgccatcgca ctctcacacc agcagggcct
     5761 ctggctgaac gtgaatgaca ttgtctgaag agggttcaac gtggaaaagc agtttgatgt
     5821 gatatgcatg ggccgcgtgg cggtagacct ctacagccag caaattggtg cccgcctgga
     5881 ggatgtgtcg agcttcgcca aatatctcgg cggctcgtcc ggcaacgtgg cgtacggtac
     5941 cgcgcgtcag ggcttacgct cgtcaatgct ggcgcgcgtt ggcgatgaac atatggggcg
     6001 cttcctgcgg gaagagttaa accaggtggg ctgcgatacc agccatctga tcaccgataa
     6061 agaacgcctc acggcgctgg tgctactcgg cattaaagac cgggacacct ttcctctgat
     6121 tttttatcgc gataactgcg cggatatggc gattactgcc agcgatgtgg acgaaagcta
     6181 cattgcctcc gcacggtgta ttgccatcac cgggactcac ctttctcacc cgcagacccg
     6241 cgaggcggtg ctgacggcgc tgaattacgc ccgccgtcac ggcgtgcgca cggtgctgga
     6301 catcgactac cgtccggtgc tgtggggact aacctctcta ggagacggcg aaacgcgttt
     6361 tatcgttgcc gaacaggtta cccgtgaact tcaggaggtg ctacaccttt tcgacgtcgt
     6421 tgtcggcacc gaagaggagt ttcacattgc ggggggcagc accgatacgt tgcaggcgct
     6481 ggcgcaggtg cgccacgtca gcaaggcgac actggtttgc aagcgcggcg cgctgggctg
     6541 ttcggtgtat accgatgcca ttccacctcg gctggatgac ggcctgacgg tgaccggcgt
     6601 gcgtgtggag gtgcttaacg tccttggcgc gggagatgcg tttatgtctg gcctgctgcg
     6661 cggttacctg aacgacgagg gctgggagca ggcgtgccgc tacgccaacg cctgtggggc
     6721 gctggtggtc tcgcgccacg gctgtgcacc ggcgatgccg agcaaaattg agctggatga
     6781 ttacctctcc cgcgccgcgc tcgttccgcg cccggacatt gacccgcgtc ttaaccatct
     6841 gcaccgggtg accacccgcc gccgcgaatg gccggagctg tgcgtgatgg cgttcgatca
     6901 ccgcagtcaa ctggaagata tggcgctgca gtgcggcgcg tcgcttaagc gtattccggc
     6961 gctgaaacag ctgatcctgc aggccagccg agaggcggca agctctgccg ggctggaggg
     7021 gaaagccggc ctgctgtgcg atggcacctt tggtcaggat gcgctgaacg ccattaccgg
     7081 cgaagggtgg tggattggtc gtcccattga actgccaggc tcgcgtccgc tggagatgga
     7141 gcacggtaac attggtaccc agcttatcag ttggccgcag gagcatgtgg tgaagtgcct
     7201 ggtctttttc catccggaag atgcccacgg cttgcgtctg gagcaggagc agaaaatcgc
     7261 cgaggtgtac cacgcctgct gtcagtccgg acatgaactg ctgctggagg tgatattacc
     7321 cgccactatg ccgcgcagtg acgaacttta tctgcgtgcc atttcccgct tctacaacct
     7381 tggcatttac cccgactggt ggaaactgcc gccgctctcc gccgaaggct ggacggcact
     7441 gagcgagatt atcgcgcgcc gggacccgca ctgccgcggc gtggtgatcc tcggtctgga
     7501 tgctccggcg gaacaactgc gtgccgactt caaagctgcc gcggggcagg cgctggtgaa
     7561 aggcttcgcc gtggggcgca cgctgtttgg cgatgcctcc cgcgcatggc tgaaacacga
     7621 tattgacgat gcgcagctgg tggcgcgcat ccgggacaac tacctgcagc ttatcgcctg
     7681 gtggcgcgag cgcggacacg cataatggag acgacaatga gtgtacaact gggcatcaac
     7741 ccgctgacgt ggacgaacga tgatctgcct tcactgggcg cggagacatc tctggagacc
     7801 tgcctgagtg aagggaaaga ggccggtttt gctggcttcg aactgggcaa taaattcccg
     7861 cgagaagcgc gcctgctcgg gcccattttg cagcgccacg acctgcagtt ggtctccggc
     7921 tggtattccg gccgcctgtt ggaacgcagt gtagaggacg agattgctgc tgtgcagtcg
     7981 catctgacgc tgctgcgaga gctgggtgca aaagtactgg tttttgcgga agtgagtggc
     8041 tgcattcacg gtgagcagca gacgccggtt cacctgcgcc cacgtttccc gcaggaatgc
     8101 tggaaagagt atggtgagaa gctcaccgtg ttcgcccgct atacgcagca acagggggta
     8161 cagattgctt accaccatca tatgggcacg gtgattgagt ccgctcaaga cgtggacaac
     8221 ctgatgctcc acacgggtga ggaggttggt ttactgctgg ataccggcca cctgaccttt
     8281 gccggggccg atccgctggc ggtggcacag cgatgggcat cgcgcatcaa tcatgttcac
     8341 tgcaaagatg tgcgcgccga cgtgctggcg gacgtcaaaa accgtaaaac cagcttcctc
     8401 gacgcggtgc tgagcggcgt gtttaccgtg ccgggtgacg gctgcgtgga ttatccgccg
     8461 attatgcaac tgctgaaaac gcaggattat cacggttggc tggtggtgga ggctgaacag
     8521 gacccggcga tcgcccatcc gctgacctac gcccgtctgg gatataacaa tctgagccgc
     8581 ctggcgcgcg acgccgggct tatctgagga ggtgacatgt cacgtctgtt atcacgctgg
     8641 caacagccga acgctgaggg gcggactcag tctgtcacgc cggaaagcgc cggatgggga
     8701 tatgttggct ttgaggccta tgaacttgag gaagggcagc aactgacgct gcccgctgtc
     8761 agtgaagagc gctgtctggt gctggtggcc gggcgcgctt tcatcagcac tccttctgca
     8821 cagtttactg atatcgggga gcggatgagc ccgttcgagc gcatcaaacc ttgggcggtt
     8881 tacgtcaccc cgcaggaggc agtgcaggtg aaggcgctga cgaaactgga gctggcagtc
     8941 tgtaccgctc cgggaaaagg gacatacccg acgcggttga ttgcacctca ggatatcgat
     9001 ggcgaagcgc gcggtaacgg ccacaaccag cgttatgtgc ataatatttt gccggaagac
     9061 aagcccgctg acagcctgct ggtcgtcgag gtctggacca acgaaggctg taccagttcg
     9121 tacccgagcc ataaacacga tacgaataac ccgcctcagg agagctatct ggaggagacc
     9181 tattaccatc gcctcaaccc ggaacagggg ttctgcatgc agcgggtgta taccgatgac
     9241 cgcacacttg atgaatgtat ggcggtctat aaccgtgacg tggtcatggt gccgaaaggg
     9301 taccacccgg tggcgacgat ggccgggtat gacagttatt accttaacgt gatggccgga
     9361 ccagtacgta aatggatatt cacctgggaa aaggaacact catggataaa cacagagtat
     9421 tctgccattt ctttataaac tgagtaccta agctaaaaaa ggggaatgac tccccttttg
     9481 tataatatga actcgacaat gttatttcaa agtctgagtg atgatttttc tctcaggcag
     9541 tatgttcctc aataaggatg agaatgtatt ctgttcattg gcatatgcat ttatattcta
     9601 atgagctgaa ttttataaag gttgtggcta ttctggagta taaata
//
LOCUS       DAPHDX010000153         9607 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPHDX010000153 DAPHDX010000000
VERSION     DAPHDX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.155"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(74..286)
                     /gene="cspA"
                     /locus_tag="RH058_003844"
     CDS             complement(74..286)
                     /gene="cspA"
                     /locus_tag="RH058_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV3139366.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(567..857)
                     /locus_tag="RH058_003845"
     CDS             complement(567..857)
                     /locus_tag="RH058_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV3139367.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1291..2001
                     /locus_tag="RH058_003846"
     CDS             1291..2001
                     /locus_tag="RH058_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV3139368.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2051..3025)
                     /gene="ghrB"
                     /locus_tag="RH058_003847"
     CDS             complement(2051..3025)
                     /gene="ghrB"
                     /locus_tag="RH058_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV3139369.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3129..3788)
                     /locus_tag="RH058_003848"
     CDS             complement(3129..3788)
                     /locus_tag="RH058_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV3139370.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3941..6274
                     /gene="bisC"
                     /locus_tag="RH058_003849"
     CDS             3941..6274
                     /gene="bisC"
                     /locus_tag="RH058_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV3139371.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMSENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6243..6683)
                     /locus_tag="RH058_003850"
     CDS             complement(6243..6683)
                     /locus_tag="RH058_003850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3139372.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6680..7243)
                     /gene="tag"
                     /locus_tag="RH058_003851"
     CDS             complement(6680..7243)
                     /gene="tag"
                     /locus_tag="RH058_003851"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV3139373.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7401..8099
                     /locus_tag="RH058_003852"
     CDS             7401..8099
                     /locus_tag="RH058_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3139374.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8328..9530
                     /locus_tag="RH058_003853"
     CDS             8328..9530
                     /locus_tag="RH058_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3139375.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2265 a         2355 c         2500 g         2487 t
ORIGIN      
        1 atttcctgaa aaacaaataa aaaaatcccc gccaaatggc agggatctta gattctgtgc
       61 ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt
      121 cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg ttctggatag
      181 cagagaagtg tacgaacacg tctttagagc catcgtcagg agtgatgaag ccgaagcctt
      241 tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt
      301 aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg
      361 aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc
      421 tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg cgggtgatgc
      481 aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg
      541 taaacagcaa taaagtggat aaaagtctat tccatcaact gcttacttaa tgccgggttg
      601 gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc
      661 cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaac
      721 cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta
      781 attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt
      841 ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg
      901 aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag ggcatgctct
      961 ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata
     1021 aatgccgata tatctcttac cactcctgtt ttgtaaagat ttcgaaaatc taaccttctg
     1081 cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa
     1141 acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag
     1201 ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt
     1261 gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg
     1321 tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa
     1381 ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa
     1441 cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac
     1501 gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg
     1561 gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg
     1621 ctgcgtgaaa tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg
     1681 caggccgatg ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat
     1741 caggcattca ccaaagtggt aacaacgcca gctgatgcat tgcaaccgtt gattccggct
     1801 gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca acaaggtact
     1861 caggtgagct ttgtagcaaa tggtatccag ttcccgactt cgcagcaggc aagtgaatat
     1921 aacaaactga ttgcgccgtt accggcacaa catcaggcgt ttaatcaggc ctggactaca
     1981 gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg
     2041 cagtcgcggc ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta
     2101 acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg
     2161 cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg
     2221 acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg
     2281 cagcaatcag tgcattttca tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg
     2341 aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg
     2401 gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg
     2461 cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt
     2521 tgaagccaaa gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc
     2581 ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt
     2641 cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg
     2701 ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt
     2761 ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg
     2821 cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta
     2881 aaccttcagc ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg
     2941 ccacctggtg aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt
     3001 tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta
     3061 gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat
     3121 gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct
     3181 gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg
     3241 gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgttg ctgggagaga
     3301 cgcatgttca ggtcgtgacc acccgtgctg tcggtataac caatcacgtt aaccgccgtt
     3361 ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc
     3421 agggtcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc
     3481 ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc
     3541 acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc
     3601 gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga
     3661 gagcccagac ctgcgccgat agcagattta cctgcttcgc gttcgccggt gtaagggtta
     3721 gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt
     3781 ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag
     3841 attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat
     3901 ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg
     3961 atattccgtt ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac
     4021 cgtgtttagc tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt
     4081 tcgcgaccag gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct
     4141 cgcgtcaccg gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg
     4201 ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc
     4261 ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc
     4321 gacattatta caacgctata tggcgctggc aggcggttat atcgggcatc tgggggatta
     4381 ttcgaccggt gcggcacagg cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca
     4441 acagcaaacc agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc
     4501 taacccactc aatacgctga aaattgcgtg gaatgcatct gatgagcagg ggctttctta
     4561 cttttccgca ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa tgcgatcgga
     4621 aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt
     4681 tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt
     4741 tctgacgcgt tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga
     4801 cggaatagcg aaaaacgccg aatgggcggc agagatttgt ggggttaacg cagcgaaaat
     4861 ccgcgagctg gcggctcttt tccaccaaaa cacgaccatg ctgatggctg gctggggaat
     4921 gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt
     4981 ggggcaaatc ggcacacccg gcggcggttt tggtctttct tatcattttg ccaatggtgg
     5041 taaccccacg cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg
     5101 cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg
     5161 cgcatatcaa cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc
     5221 gggcggtgcc aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa
     5281 accggagctg gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat
     5341 cgttctgcct gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag
     5401 taatcagcat ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga
     5461 ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac
     5521 ggaaggaaaa agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg
     5581 ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat
     5641 tgagatgtcg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga
     5701 tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat
     5761 tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg
     5821 gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct
     5881 gcacagccag ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc
     5941 tgtcaccatt catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg
     6001 ggtgtggaac tcccgtgggc agatccttgc cggagcggtc attagcgagg gaattaaacc
     6061 tggcgtgatt tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat
     6121 ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa
     6181 tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac
     6241 acttacagcg tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc
     6301 tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga
     6361 tttttttgat aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc
     6421 gccttaccaa taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg
     6481 ccttccataa tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc
     6541 gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt
     6601 ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct
     6661 gaacgttgcg cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat
     6721 gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact
     6781 taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa
     6841 tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa
     6901 agtcggcaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa
     6961 ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat
     7021 cctcttcctg cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt
     7081 tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga
     7141 tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccattcatta tcatggtagg
     7201 caatataaag cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt
     7261 acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta
     7321 atacagggct actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc
     7381 tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc
     7441 tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag
     7501 aatatatcgt tgatacgcaa cccgggcatt ccacagagcg ttacacctgg gatagtgatc
     7561 atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg
     7621 gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg
     7681 gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg
     7741 atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc
     7801 cgttatggca tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc
     7861 tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc
     7921 aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg
     7981 taggcgctga tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag
     8041 aaaataccac taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac
     8101 gtaacagtca caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact
     8161 taccaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt
     8221 cattccgttc tgatggcatt tcatgccgtt tttccccagg cataaagtgc acttcgttat
     8281 ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa
     8341 attatcagcg tacccgctgg ctgacactca tcggtacaat cattacccag tttgcgctgg
     8401 ggtcggttta tacctggagc ctgtttaatg gcgcgctttc cgccaagctg gatgcgccgg
     8461 taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg
     8521 ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt caccatggct tccggcattt
     8581 tgctgggatt aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa
     8641 gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact
     8701 gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg
     8761 gtctgggtag cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtttgg
     8821 aaaaaacatt tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt
     8881 taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt
     8941 acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga
     9001 ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg
     9061 cacaccttga tgtggtttcc gcagccaatg cagtcactgt tatttccatc gctaaccttt
     9121 caggtcgtct ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca
     9181 ttggtcaggt gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag
     9241 tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct
     9301 ttccatcact ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga
     9361 tttacctcgg tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg
     9421 gtttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta
     9481 cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc gcatatttag aaatatgttg
     9541 ctactgaaat aaatacgagt agggagactt caaagtctcc cttttttatg gacgcatatt
     9601 aaaagaa
//
LOCUS       DAPHDX010000154         9351 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPHDX010000154 DAPHDX010000000
VERSION     DAPHDX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.156"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(7..114)
                     /locus_tag="RH058_003854"
     CDS             complement(7..114)
                     /locus_tag="RH058_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3139376.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(519..1619)
                     /gene="chaA"
                     /locus_tag="RH058_003855"
     CDS             complement(519..1619)
                     /gene="chaA"
                     /locus_tag="RH058_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV3139377.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1889..2119
                     /gene="chaB"
                     /locus_tag="RH058_003856"
     CDS             1889..2119
                     /gene="chaB"
                     /locus_tag="RH058_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV3139378.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2277..2972
                     /gene="chaC"
                     /locus_tag="RH058_003857"
     CDS             2277..2972
                     /gene="chaC"
                     /locus_tag="RH058_003857"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV3139379.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(3016..3369)
                     /gene="ychN"
                     /locus_tag="RH058_003858"
     CDS             complement(3016..3369)
                     /gene="ychN"
                     /locus_tag="RH058_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV3139380.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3554..4948
                     /gene="ychO"
                     /locus_tag="RH058_003859"
     CDS             3554..4948
                     /gene="ychO"
                     /locus_tag="RH058_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV3139381.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4949..5599)
                     /gene="narL"
                     /locus_tag="RH058_003860"
     CDS             complement(4949..5599)
                     /gene="narL"
                     /locus_tag="RH058_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV3139382.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5592..7388)
                     /gene="narX"
                     /locus_tag="RH058_003861"
     CDS             complement(5592..7388)
                     /gene="narX"
                     /locus_tag="RH058_003861"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV3139383.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7727..9118
                     /gene="narK"
                     /locus_tag="RH058_003862"
     CDS             7727..9118
                     /gene="narK"
                     /locus_tag="RH058_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV3139384.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2276 a         2284 c         2394 g         2397 t
ORIGIN      
        1 ttcaccttac ttccggttac gccaccagct gacaatcgct gcggtaataa tccccgccag
       61 gatcggtgct gccaggtcgt gccagaaagt catggcaaac tgcgcgagcg tcatatagcc
      121 gccttgttgt aatgacaaca ttttgcggct attcttgaag tgtctggttt caagattagc
      181 ccccgttttg ttgtcaggtt aaacctctca acgtgcgggg gttttctctt tccagcaacc
      241 aatgccacca gggataaagc ccccgcaaca ttgcgcctca ccggataatt ccggcttggt
      301 gtggatacta cgactcaatt catcttcact tcatccctga aatgtttgca ataaagagta
      361 cattccggct tttcaacagc tgttgcagtg gtttcgtgca ggttgtggta caggctcgca
      421 attctgatta acgagctgtc caggtagtat gaagaaatac aataagcagg aatttatctt
      481 atctcccccg atttccgtca tcggaggaga tataactgtc aggcaaatat cgtcatcaaa
      541 taggcggcaa acagtgccag atgcgctgcg ccattgagca cgttagtacg tccggtggag
      601 aaggagatat ggcacagcac taaagaggcc accatcacca ccatttctgg cgcaccaagt
      661 gcaaactgta attcgttacc cgtcataaag gcaattagcg tgacgacagg tacggtaagc
      721 gaaatggttg ctaacactga accaaagaac agattcatcg cgcgctggac ctggttgttc
      781 aacactgctt ttaacgcacc taaaccttcc ggcgacagaa tcaacagagc caccaggaag
      841 ccagtaaagg cgacaggggc attcatgctg tcgagcaatg tctccagcgg gctggcattc
      901 attttggtga ctgcaataac ggcaatcaga tggataatca accagatagc atgccacacg
      961 ctgctatggg cagacggttt accgtgatgc gggtcatcgt catcactgtc atcttcgtgc
     1021 tcgtagacaa acaaactttg atgcgttttg gtctggatca gcaaaaatac gccatacatt
     1081 gccgcagaaa ttaatgctac cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga
     1141 gccattggaa ataccagtac gattatcgcc agggggaaca gcgcaattaa atactgcttg
     1201 ataccaaaca gattcatata ttgggtggca aacttacgac cgcccaacaa taatgaaaag
     1261 ccaaccagcc caccggtaac aatcataatg attgaataga gcgtatcacg cattagcgtt
     1321 ggcgcggcgt cgccggttgc cattaaagct gaaatcaaac tgacttcaag aataaccact
     1381 gaaaggctaa gaataagcga accgtaaggt tctcccaggc gatgggctaa tacgtccgca
     1441 tgacggacaa cactaaaggc gctacttaaa ataccaataa gcgcaagaag attgatggca
     1501 atgaccactg gtagtgtctg gctacttccc cacaggaaca gcactaccag cgccagaacc
     1561 gggaaaataa gcgaagtctc cttgtggcgg gtttttaccg cctcttgagc atttgacatt
     1621 atggttatcc ctttgcagat gaatttatcg aaaatgtaaa aaataggtag ggaaaataac
     1681 agaaagtgtc tggatatcgg taacatttta cgaattttta ccccgctgtc gattttttac
     1741 tcattggggc ataaaaataa gtattacgtt tagacaatgt ttgttttagc gtttcttaag
     1801 aagagtctga catgaaaatt cttatgtttt ggcaagagta gatattgttg accacactta
     1861 atgttcaact ttgtaaaagg agtcaacgat gccgtataaa acgaaaagcg atctgccgga
     1921 aagcgtaaag cacgttctac cgtctcatgc ccaggatatc tataaagaag cgttcaacag
     1981 cgcatgggat caatataaag ataaagacga tcggcgtgat gacgccagtc gcgaagaaac
     2041 cgcgcataaa gtggcctggg ctgctgtgaa gcatgaatat gccaaagggg atgatgataa
     2101 atggcataaa aaatcgtaaa accggtagct tagttaaagc tattcgtgcg gtgttgcctt
     2161 gcaagtggtc cgtggattgc atattgtccc gttagtggtt tcaaaatgag cagtaaaaat
     2221 gtccggaaga caccaaaaag ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga
     2281 taacgcgtga tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat
     2341 cactcttatg gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac
     2401 ctgatgagga accggtgtgg atcttcggct atggatcgtt gatgtggaat ccggcactgg
     2461 agtttaccga atcgtgcacc ggtacactgg ttggatggca tcgcgcattt tgcctgcgcc
     2521 tgaccgccgg gcgcgggact gcgcaccagc cgggacggat gcttgcactg aaagagggcg
     2581 gacgcaccac aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaacct
     2641 tgttgtggaa gcgagagatg attaccggct gttatctgcc aacctggtgt cagcttgatc
     2701 ttgatgatgg acgcacagta aacgccattg tgtttattat ggatccgcga catccagaat
     2761 atgaatctga tactcgcgct caggtcatcg cgccgttgat tgcggcggcg agcggtccgc
     2821 tgggaaccaa cgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc
     2881 aggacgatgg gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattttc
     2941 cggatggtgt gttacgtccg ggattcgcct gagtaaactt cccgcatagt ggggcgtcag
     3001 acgccccctc aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag
     3061 ttccacaaga gtaccgattt ccaccccatc aatcagagga agtgtactaa tcccgcgccc
     3121 gtcggtacag gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat
     3181 ttgctgaatg ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc
     3241 agacatgagg aacagacgca gatccagatt gctctcctgc tctcgtaacg caatggccag
     3301 ccgcaagctg ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat
     3361 tttttgcatt atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct
     3421 tctgcagcgt ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat
     3481 acgacaaaag ttgccagtaa tcgttattct ttaaggctat ggtttttcat tattaccgga
     3541 agttaccgac gttttgagcc gtttcgttcc tcgcattatc ccgttttatt tactcttgct
     3601 tgtagcaggc ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatccctt
     3661 tgataataac aatgatggtc tgccggattt aggcatggct cccgaaaatc atgatgggga
     3721 aaaacacttt gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct
     3781 ggatactggc gagcaggcaa aagctttcgc attgggaaaa gtccgcgacg cgcttagtca
     3841 acaggttaat cagcacgtag agtcctggct atcaccgtgg ggaaatgcca gtgttgacgt
     3901 caaagtggat aacgaaggac atttcaccgg cagtcgtgga agctggtttg tgccgttaca
     3961 agataatgat cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggtt
     4021 ggtgagcaat gtgggcgttg ggcaacgctg ggcgcgcggc aactggctgg tgggttataa
     4081 cactttttat gacaacttgc tggacgaaaa tcttcagcga gcgggctttg gtgccgaagc
     4141 gtggggcgaa tatttgcgac tatcggcaaa cttttatcag ccgtttgctg catggcatga
     4201 acagacagcc acgcaggaac aacggatggc gcgcgggtat gacctgacag cccggatgcg
     4261 catgccgttc tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg
     4321 tgttgatttg tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt
     4381 aaattacacc cctgtgccat tagtcactgt gacggcccag cataaacagg gtgaaagtgg
     4441 cgagaatcaa aataacctcg ggctgaatct taattaccgc tttggtgtac cgctcaaaaa
     4501 acaactttct gcgggtgagg ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa
     4561 tccgcagcga aataatctac cgactcttga gtaccgacag cgaaaaacgt taacggtgtt
     4621 tctggcgaca ccgccgtggg atctaaaacc tggcgaaaca gtgccgctga aattacaaat
     4681 ccgcagtcgt tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt
     4741 gacgccgggc gcacaagcca acagcgcgga gggctggacg ctgatcatgc ctgactggca
     4801 gaacggggaa agggcgagca atcactggcg attgtctgtg gtggtggaag ataaccaggg
     4861 gcagcgtgtc tcctccaatg agatcacgct aacgcttgtc gaaccgttcg atgcattgtc
     4921 aaacgacgaa ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg
     4981 ctgcttccac gcgagacttg agcttcattt tcttcagcat gtgcttgacg tgcactttta
     5041 ctgtgctttc ggtgatgtcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa
     5101 tcagcttgag aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg
     5161 cacggttagc acgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt
     5221 cgccagcagc tgcctgatgc aatgctttca gcagatcttc cggttccata tcttttaaca
     5281 gatagccatc cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc
     5341 tgaataccac aatgcgccct gagagggact tttcgcgcag tttatccagc gtttccagac
     5401 cgttcatgcc gggcatattg agatctaaca ggatcagatc gggatcaaga gactccgcca
     5461 gttcaatacc ctgttcgcca ttactcgctt cgccaaccac ggtgatatct ggtgccatac
     5521 tgataagctg ttttacgcca gttcgcagca tcgggtgatc gtcaatcagc aggatagtag
     5581 ccggttcctg attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata
     5641 aaggtgacca ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt
     5701 aaactttgcg cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt
     5761 tcaggcacgc cgcagccgtt atcctgtacg gtcagtttga cctgattatc gttttgcgcc
     5821 accgtcacca cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg
     5881 gcaatttgca acaagtggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa
     5941 tccagcttca ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc
     6001 ggacgtaatc caggctcggt gagctgcaag cggaatgtgg tgagcaattc acgcaactgc
     6061 gcccaggatg cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc
     6121 agcgcatcgc cctgcatctg taaacaactc acctgcatct tcatacaaga gagagattgg
     6181 gcaatagaat catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt
     6241 tgctgacgtt cctgatggcg atccaacgcc agcgtggcgg tgagttgttc aaccagggta
     6301 tccaccagtt gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa
     6361 ataccgtact gcgtatgaga gtcagccagc cgccacttca gggttgtgcc gcgatcgcca
     6421 acgggtaata cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctggc
     6481 tggcaggtaa actcctgatg attctcttca tcatcagtat cataaacccg cagttcgata
     6541 tcacgtagca gggttaaatt ctgtaagccg ttgagtaccg gtgacaggcg ttcacacagc
     6601 ggggcgcggg aatgcaaacg gcggttagcc tgccataaaa aagagaggat ctgattttta
     6661 tgctccagcc cggcggtttt ctcctgaacc cgctgctcaa gtacggcata actttcggcc
     6721 agttctgcag acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg
     6781 atgtttgcgc gttgggtaaa atcgcgatgg ctgacggcac tcgccattgc cagcagttgc
     6841 cgccacggtt gtagcagtcg cgcccgcaac cagataatag tgaacaccag taaaagtgcc
     6901 ataaataccg ccattacccg atggaccagt accactgtct cgatgcgcat ttccgtggtg
     6961 cggtcaaaac cagataccag ctgatcaagc ccggcaacaa actggctgac atccgctgac
     7021 accgtttctc ggttttgtgc acgcatcagc gcagggatca gttcattacg ccagtaatct
     7081 tgtaaaccct gtaattgcgc cagttgtccg tctcgttctg ctgctcgagt caactcggcg
     7141 ctaaatgccg tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact
     7201 gccgccaaca gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg
     7261 ctgccctgaa cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca
     7321 gtagaaagca acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt
     7381 ttaagcatgt aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac
     7441 gatgcattct ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa
     7501 gagttactcc ttatttgccg tgtggttagt cgctttacat cggtaagggt agggatttta
     7561 cagcaccgtg aaaaatctca taatttttat gaaatcactg tactcactat gggtaatgat
     7621 aaatatcaat gatagataaa gttatcttat cgtttgattt acatcaaatt gcctttagct
     7681 acagacacta aggtggcaga catcgaaacg agtatcagag gtgtctatga gtcactcatc
     7741 cgcccccgaa agggctactg gagctgtcat tacagattgg cgaccggaag atcctgcgtt
     7801 ctggcaacaa cgcggtcaac gtattgccag ccgtaacctg tggatttccg ttccctgtct
     7861 gctgctggcg ttttgcgtat ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt
     7921 cggttttaat tttacgaccg atcagctatt tatgttgact gcgctgcctt cggtttctgg
     7981 cgcgttatta cgtgttccat actcctttat ggttcctatc ttcggtggtc gtcgctggac
     8041 ggcgttcagc accggtattc tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga
     8101 tacctccacg ccttatagcg tcttcatcat catctctctg ctatgcggct ttgctggcgc
     8161 gaacttcgca tccagtatgg caaacatcag cttcttcttt ccgaaacaga agcagggtgg
     8221 cgcgctgggt ctgaatggtg gtctgggaaa catgggcgtc agcgtcatgc agttggttgc
     8281 tccgctggtg gtatcactgt cgattttcgc agtatttggt agccagggcg tcaaacagcc
     8341 ggatgggact gagctgtatc tggcgaatgc gtcctggata tgggtgccgt tccttgccat
     8401 cttcaccatt gcagcgtggt ttggcatgaa cgatcttgct acctcgaaag cctccatcaa
     8461 ggagcagttg ccggtactca aacggggtca tctgtggatt atgagcctgc tgtatctggc
     8521 aaccttcggc tcctttatcg gcttctccgc gggctttgcg atgctgtcaa aaacgcagtt
     8581 cccggatgtt cagattctgc aatacgcttt cttcgggccg tttattggtg cgctggcgcg
     8641 ttctgcaggt ggtgcattat ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt
     8701 tattctgatg gcgattttca gcggcctgct gttcctgacc ttacctactg acgggcaggg
     8761 cggaagcttc atggcgttct tcgcggtctt cctggcgctg ttcctgacag ctgggctggg
     8821 tagtggttcc actttccaga tgatttcagt gatcttccgt aaactgacaa tggatcgcgt
     8881 gaaagcagaa gggggttctg acgaacgtgc gatgcgtgaa gcggcaaccg acacggcggc
     8941 ggcgttgggt ttcatctctg cgattggcgc gataggtggc ttctttatcc cgaaagcgtt
     9001 cggtagctcg ctggcattaa cgggttcgcc agttggcgca atgaaagtat ttttgatttt
     9061 ctatatcgcc tgcgtagtga ttacctgggc ggtatatggt cggcattcta aaaaataaac
     9121 cgttactcgt catacttcgg gttacatgtg ctgcggctgc gttcattcac cccagtcact
     9181 tactttagta agctcctggg attcattcac ttgccgcctt cctgtaaacc gaattatata
     9241 gagtaaaata tttgattatc ctttgcgcgg catgatgtcg cgcttttttt atgcgtcatt
     9301 tagttacaac atactcatat tatatggctt atttcgccgg atttcattaa g
//
LOCUS       DAPHDX010000155         9219 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPHDX010000155 DAPHDX010000000
VERSION     DAPHDX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.157"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(106..1749)
                     /gene="yeeO"
                     /locus_tag="RH058_003863"
     CDS             complement(106..1749)
                     /gene="yeeO"
                     /locus_tag="RH058_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV3139385.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1754..1829
                     /locus_tag="RH058_003864"
     tRNA            1754..1829
                     /locus_tag="RH058_003864"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Asn,seq:gtt)
     gene            complement(1867..2817)
                     /gene="cbl"
                     /locus_tag="RH058_003865"
     CDS             complement(1867..2817)
                     /gene="cbl"
                     /locus_tag="RH058_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV3139386.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMESSEEEIDYQI"
     gene            complement(2919..3836)
                     /gene="nac"
                     /locus_tag="RH058_003866"
     CDS             complement(2919..3836)
                     /gene="nac"
                     /locus_tag="RH058_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV3139387.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4163..4238
                     /locus_tag="RH058_003867"
     tRNA            4163..4238
                     /locus_tag="RH058_003867"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4196..4198,aa:Asn,seq:gtt)
     gene            complement(4294..5229)
                     /gene="ldtA"
                     /locus_tag="RH058_003868"
     CDS             complement(4294..5229)
                     /gene="ldtA"
                     /locus_tag="RH058_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3139388.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5291..6370)
                     /gene="cobT"
                     /locus_tag="RH058_003869"
     CDS             complement(5291..6370)
                     /gene="cobT"
                     /locus_tag="RH058_003869"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV3139389.1"
                     /translation="MQTLADLLNTTPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6382..7125)
                     /gene="cobS"
                     /locus_tag="RH058_003870"
     CDS             complement(6382..7125)
                     /gene="cobS"
                     /locus_tag="RH058_003870"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV3139390.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7122..7667)
                     /gene="cobU"
                     /locus_tag="RH058_003871"
     CDS             complement(7122..7667)
                     /gene="cobU"
                     /locus_tag="RH058_003871"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV3139391.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2555 a         2456 c         1963 g         2245 t
ORIGIN      
        1 gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt
       61 ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacaac
      121 tggctttttt tcacactttt gcggctcggg tcgagggtat ttccatagcc aacgtccagt
      181 aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca
      241 gacaccaacc acaccccagc caagcatgat tcccagcaca taaccgacta caacccgaca
      301 accccacatg ctcaacatcg aaacccacat ggcgtaacgg gcatcacgag cacctttaaa
      361 tccagcgggt agcacccatg aggcggacca aataggcata aataaagcat ttagccaaat
      421 cagaatcaca acgacatgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc
      481 ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggaaa gccagaacac
      541 atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat
      601 gatcgtagaa gcagagccga gcgcacttcc gggtaagttg ataagagccg caattgaaaa
      661 cgcgataaaa tttccggcaa taacactggt ccccatcccg gcaacgaaca tttgggttaa
      721 taaccgacca ctggtaaata acactgattc gacactcgcg ggaataccaa tccccatgac
      781 ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc
      841 aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat
      901 ggttaaaccc agccctgccc cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat
      961 caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca atagcggtat
     1021 tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg cagctgctgg
     1081 ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt ctgtcgtggc
     1141 atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaataagcg ttgccaacag
     1201 tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg
     1261 atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga tagcagcaaa
     1321 aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca tcgcatcttt
     1381 tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac aggcattctc
     1441 catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt tgtagctctt
     1501 gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg agatattcaa
     1561 agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag aatcagcaaa
     1621 gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg atagaaacgg
     1681 caaacagttg gggaatttaa aaatcgggtt tgacaaaaga tttttcgccg ttaagatgtg
     1741 cctcaacaac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     1801 cactggttcg agtccagtca gaggagccaa attcctgaaa agcccgcttt tatagcggga
     1861 tttttgctat atctgataat caatttcctc ttcactgctt tccatcacct gccgcttgat
     1921 atcctcaact gacagtcctg cattacaaag ttccagaaag cgccagacat agttacgctg
     1981 aagttgtcct cgcttcagtc ccaaccagac agtattagca tcaaaaagat gccgcgtatc
     2041 caggcggatt aaattctctt cctcttgttc gccactggat tgctcggcaa ctaatccgat
     2101 cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac ttaatacaat
     2161 atctgccagc aaacctttgc gggcaaatgc gtcgtcaata cgtgagcgcc ccgtaatccc
     2221 ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg gtgaaatttg
     2281 cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc caacgaaacc acgggaaggc
     2341 gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag cttcgccatt
     2401 ttgcaacaat gtcgcaattt cctgtggcgt cccctggatt agctcgagcc gaacctccgg
     2461 gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct gagtatgcgt
     2521 cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa gccgacgaac
     2581 attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc ccggttcagt
     2641 catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt catcctccag
     2701 ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg caacctctgt
     2761 caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga aattcacggt
     2821 aaactccggg cagttcagat ttcccgttat tgttaaagtc taatgcccgg cataacaaat
     2881 aataaaaacc cgcatcttat tccatcccga tataacactt agctcaccaa ttgccactgc
     2941 cttttttcca tcactggaga actaatcact gacattaaca actctttcac tgcctgtgcc
     3001 tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact catggaagga
     3061 gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc ggccgattcg
     3121 ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtggc aatagactca
     3181 atttcaccaa taacttttgc cgtgagtcgc cgtagggaaa aagcctcatc aacacgaagt
     3241 ctaatagcac tgtaatcact ggggagaaag aggttcattt gcgcaatagc attcacatca
     3301 acgctttgcc ccgggcaatc ttgagttcct accagaaaaa gatcttcttt cagcaaagcc
     3361 tgactggata caccagccac aggggaatgc tcataaatca ccgccatatc gagttggtga
     3421 tttatcaatt tttcgttaag cactgcacca ctattttcat gaagatagat aacgatctcc
     3481 ggaaattcag cgcgaaccgc ctgcaataag ggcatggtga tggatgacgc agcggttcct
     3541 ggtgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg caccgccagt
     3601 tgggcctgtt cacactgacg taaaatggcc cgcgcatggg tatagagaat ttttccggcg
     3661 tctgttggtg taacgccccg ctttgtacgg atcaaaagtt gttgatttaa ctcaccttcc
     3721 agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc agcagcctgg
     3781 gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg
     3841 cctccggttt ttaagaatcg gcccaagtgc cgccattact tacaaccaga ttgcaagatg
     3901 cttgccagtt ttattttggt gttgatgtac aagctaacca actgtcaaat aagagattat
     3961 gatagattcg tcatttgctc ctttaatcag ctgtcgcgtt cccctgccct ataaaaggag
     4021 ggtatgcacc acgatggttc attacccaat aagattgaaa gctcaccact ttgttgaaat
     4081 tggcagcaaa caaacaaaaa aatgcatttc accctttgac atcaccatgc actgccatta
     4141 atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     4201 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag cctgctttct
     4261 agcaggcttt ttgctttcta attaccaacg ctcttaaaac atctgtcttg aaccagaact
     4321 aatttgcaca ggcattcccg atcgacgttg caacgcagca tttgcgcgat tcacatcaac
     4381 ttcttgcccg ttgataaacg cccgcaaaga tggggttacc ggcaatggca cttttcggtc
     4441 agactcatat tctgcacgat tgcgcgacag cggctcatga acttccagcc agttcgagcc
     4501 atctggttca gtggtgtatt ttactggctg gtcgataatt tgcacacgcg ttccaacagg
     4561 aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc cctgacttac
     4621 ccgaagtcca ataccaaaat tggcattggt accgtggatg gcatacaacc tgccaatata
     4681 aatcgcgtac agccccatgg gattatcggg ccccgcagga acaaatgcgg gcaaactctc
     4741 ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc gtccaggttg gagcttcttg
     4801 tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct ggccgatacc
     4861 aataggaaag acttccacag tattactgtc tggcgggtag taataaagac gcatctcagc
     4921 gacgttaacc acaatccctt tacgaacagt ggcgggcaaa atcagttgct gcggaatggt
     4981 gagttgcgag ccagacttcg gcaagaaaac atcagcgccc ggattcgctt ccagcatgtt
     5041 acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgggtat tgtgatcagg
     5101 aacagttaca gtaagcgact gccccaccaa acggctaccc tctggaggta atggataagt
     5161 taccgccagg ctagtatggc tggcaaaaag cagagcaaat gagcaaagaa tatttacacg
     5221 acgcatcatg tccctttcct atgtcgcgaa agctatccgt taagtatagc ttttatcaga
     5281 cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca atattactgg
     5341 cagcaagttc gcccatgttg ttatatatcg cacaggcagc ttcgatgatg ggcatcgcca
     5401 gagctgcgcc actcccctca cctaaacgca tctccatatt gagataaggc tccagcccca
     5461 aatgcgacag cgctatacgc gccccttttt ctgccgacag gtgagaagga atgagatacg
     5521 gtttgattgc aggagacatc tggcaggctg cgagcgcagc agcataagaa agaaatccat
     5581 ccagcaacac gggtaaaccg caggaagcag cgcctaacat cacgccagcc attccgacca
     5641 aatcaaatcc ccccactttt gccagaacat caacgccatc ctgcggattt ggttgattca
     5701 acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc ggcaggtttg
     5761 cgccaatccc aaccacttct tcaggatccc ggccagtgat tgtgctgact attgccgctg
     5821 ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtgaca ccgttttttg
     5881 ccagctcccg cgtataacat atgacgtcca aaagcaactt ttcagcctga cggcgactca
     5941 ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac acgcatgttg ataagcccgg
     6001 ggataggctc agcactatca ataccaacat caactacgtg gacgttagcg cccgcttgtg
     6061 ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga atggctgtca
     6121 cttcttttgg ggaaatagcg accccttcct cccagacgcc gtgatcggca cacataacca
     6181 gtaccgcttt tttgcccaca tgcggtatgc cattcaaccc cggcattccc gccagttgta
     6241 tggcaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg tcaatatgcc
     6301 gttgtgcacg cgacatagcg gcaggatcga tagcaggggt cgtattcagt aaatcggcaa
     6361 gtgtttgcat gtcacgtctc tttatagcag tgccagcagg aagaccagtt caccaagctc
     6421 tatagcagct cccagcgtat cgccggtttg cccgcccagc gtacgtttga gaagctgacc
     6481 gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca tacccggcaa
     6541 taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc gcccatcaat
     6601 tttgccaatg aacacattgc caagcccttc ttcccgggca taacgatgac gatacatcaa
     6661 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     6721 gccacgcaga gccaactcgc ttaataccag aatctttgcc agtacaacaa aaatcaatgc
     6781 cagaccgcca tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     6841 acgtgcagaa aatacgccat cacaggtatc ggcgaggccg tccagatgga atcccccggt
     6901 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac accatgcctg
     6961 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatta aaggaaaagt
     7021 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     7081 cagacgcgta ataaatgaga gcatcgccca aaataattta ctcatttgat ttttactcca
     7141 atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccagctgctg atttacccgc
     7201 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     7261 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     7321 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     7381 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag caacacagct
     7441 tcatgcgggt taatgtctgc atgaattaat tcatcaagat gttgccaacg ctccaccgtg
     7501 cgccagtgcg ccggacggcc ttgccgatga tgttctatcc gtgcagccat ctcgtcatca
     7561 aggatttgcg aggtagcgat atacagaacc tgtgaagagt ccccaataag cgcctctgcg
     7621 tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa tcatcatacc gactcctgat
     7681 tcaaatgtat gatggtattc attgaacaga acaatggatc ccccaccgtt gctgaccgca
     7741 aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat tctcaatcca
     7801 ttacgaaata agatggcatt acggaatagc cagactttct cgtgttaacg tccgacaaaa
     7861 tgcgtcattg acagcggttt ctgtggattg acacatttca ttcagataat gaattaatgc
     7921 tactgccgga ccaaccagca aacgggcatt gtgttctgaa atccaggcta ttgattcaac
     7981 gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca acgcaataga attatccgga
     8041 atggtggcga ctatgcacta gaacaccaca atttcgctct ctcggtaagg cactgtcaca
     8101 gcattcaaca gaatgtgact tgccagattt attagcgcca ccagatgttt aaccgggtaa
     8161 ccacgaccca gtcggggata tattccatct tctcgctgac atcatcaaga ataaaaaggt
     8221 ttatcacact cagagcaacg ttcttcagca ggcgggataa ttcttcaaca tttagaagat
     8281 gtgttattaa aggcatacaa actggacgta ttgtattttc ttttggtggt tgacctaagg
     8341 tagcagttta tcctgatgcg ctgagatttc tgtaatatct acgtcaacat tctcctcgaa
     8401 tagaaaatgc agccccggca agtgattcac attttttaac aaaacgttgt tagcgtgcca
     8461 ttttcagaag ctctccaaga aagcgaattc ttttctcaaa ttctgcattc tcagacacag
     8521 ctttctactc catggcaatg gcccaaatac gaagttgctc aggatcatta gtggtgctga
     8581 gaagttaaat aaccatgcgg agtaccactc gtcatcacgt atttatatat catgaagcta
     8641 ttgttgtgtt ttgtacatca gtagatatat tctgatatac tccttttgct agacataacc
     8701 tttcacctgc ttgcaaagct tctgtgttct gacattgcca aattgttgca attctgtatc
     8761 cagccttctt tcagtcatag cttcgggccg cgataagact cactgatctg accctgattc
     8821 ctcttgcaga ctttatagac caattaaaat gcagtttctg caggtcaacg tctgaccatc
     8881 attgtcatca ctctggccat tagagtaacc ttctgcattc atccttttgt aaaaagttta
     8941 tatcagtatc agcaattaac cggacctgat actgatatga gtcttaccgc atatacggtt
     9001 aatttcagca attaattaca ttatccacgc caaagtattt gtcatcacaa tgatggtacc
     9061 ttctttcaga caccattttt tcaactccgt tttccacgga ccgcactctt atgtcaagag
     9121 tgcggtccgt ggatacaacc agagaccgac tgacacgagt cagaggaaac gacggatatg
     9181 ttcagtcgta aaatatctat caaaaaacat gattaaggt
//
LOCUS       DAPHDX010000156         9202 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPHDX010000156 DAPHDX010000000
VERSION     DAPHDX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.158"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..181)
                     /locus_tag="RH058_003872"
     CDS             complement(<1..181)
                     /locus_tag="RH058_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV3139392.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMA"
     gene            214..882
                     /locus_tag="RH058_003873"
     CDS             214..882
                     /locus_tag="RH058_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3139393.1"
                     /translation="MSKLAASPKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCS
                     MFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPRRPN
                     FPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLP
                     VTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIK
                     GSSH"
     gene            882..1229
                     /gene="tnpB"
                     /locus_tag="RH058_003874"
     CDS             882..1229
                     /gene="tnpB"
                     /locus_tag="RH058_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3139394.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1249..2820
                     /locus_tag="RH058_003875"
     CDS             1249..2820
                     /locus_tag="RH058_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV3139395.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPHETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPEVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWGALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
     gene            complement(2852..3291)
                     /locus_tag="RH058_003876"
                     /pseudo
     CDS             complement(2852..3291)
                     /locus_tag="RH058_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3899..7312
                     /locus_tag="RH058_003877"
     CDS             3899..7312
                     /locus_tag="RH058_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV3139396.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEGLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYDQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTGFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWHQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGPRINQLRD
                     LGWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTENRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQSELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            7376..9010
                     /locus_tag="RH058_003878"
     CDS             7376..9010
                     /locus_tag="RH058_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV3139397.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGEKLASLLKQQYPEGIDAGVDFKDRTQAAFIVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
BASE COUNT         2417 a         2330 c         2423 g         2032 t
ORIGIN      
        1 tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca
       61 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc
      121 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
      181 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcaccgaaa
      241 caccggacct ggatcactga agctttacgt cttcactttg aagaacattt accccgggtt
      301 gtggccgggc gtcgcctggg tgtaccaaaa tcaacagttt gtagtatgtt cgtgcgcttt
      361 cggagagctg gcctttcgtg gcctttgccc gcaggcatgt cggagcagga acttgatgcc
      421 tgcctttacg gacaattttc cacggtacca gtcgtacgtc ctgaaagcac cgttatatcc
      481 gaagcccccg tggtaaaaaa acgtccccgg cggcccaact tcccttatga gtttaaaatc
      541 gccttagtgg agcagtcact gcagcccgga gcctgtgtgg cgcagatcgc ccgggaaaac
      601 ggaatcaacg ataacctgct cttcaactgg cgccatcaat accggaaagg tggcctgctg
      661 ccttccggaa aaaatatgcc ggcactgctt cccgtgacgt taacgccgga gccggataat
      721 aaaatcccgg cccccgcaca ggaaccagag cagataaata caccgtccga cagtctgtgt
      781 tgtgagctgg ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta
      841 ttacagacac ttatccgcga aataaaaggg agcagccact gatgatatct ctccctgcag
      901 gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg
      961 catcaaaagt tcagaacgtc ctgaaggatg acccgttctc cggacacctg ttcatcttcc
     1021 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
     1081 tcaccaaacg cctggagcgg ggccgcttcg tctggccagt cacccgtgac ggcaaggtgc
     1141 accttactcc ggctcagtta tccatgcttc ttgaaggtat caactggaag cacccgaaac
     1201 gaacggaacg cgctggaatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
     1261 cttgctcatg agaacgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
     1321 cagatggcga aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc
     1381 aaccggctga aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa
     1441 aaacttcgcg ctaaaaccga acggcagata caggaagcac aggagcgaat cagcgcactt
     1501 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atccgccctg
     1561 cgccagtctt cagcccgtaa accgttaccg gcctcacttc cccatgaaac ccgggtcatc
     1621 cggccggaag aggaatgctg tcctgcctgt ggtggcgaac tcagttctct gggatgtgat
     1681 gtgtcagagc aactggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
     1741 aaactggcct gttgccggtg cgaccatatc gtgcaggcac cagtaccttc aaaacccatt
     1801 gcacgcagtt atgccggagc agggcttctg gcccatgttg tcaccgggaa atatgcagac
     1861 catctgccgt tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt
     1921 gccacactgg ggcgctggac aggtgctgtt gctgaactgc tggagccgtt gtatgacgtt
     1981 ctgcgccagt atgtgctgat gcccggtaaa gtccatgctg atgatatccc cgtcccggtc
     2041 caggagccgg gcagcggtaa aacccggaca gcccggctgt gggtctacgt ccgtgatgac
     2101 cgtaacgccg gttcacagat gcccccggag gtctggttcg cgtacagccc ggaccggaaa
     2161 ggcatccatc cacaaaatca cctggccggt tacagcggag tgcttcaggc cgatgcttac
     2221 ggtggctacc gggcgttata cgaatccggc agaataacgg aagccgcgtg tatggctcat
     2281 gcccggagaa aaatccacga tgtgcatgca agagcgccca ccgacatcac cacggaagcc
     2341 ctgcagcgta tcggtgaact gtatgccatc gaggcagagg tccggggctg ttcagcagaa
     2401 cagcgtctgg cagcaagaaa agccagagcg gcaccactga tgcagtcact gtatgactgg
     2461 atacagactc agatgaaaac actgtcgcgt cactcggata cggcaaaagc gttcgcatac
     2521 ctgctgaaac agtggggggc cctgaacgtg tactgcagta atggctgggt ggaaatcgac
     2581 aacaacatcg cagagaacgc cttacgggga gtggccgtag gccggaaaaa ctggatgttc
     2641 gcgggttccg acagcggtgg tgaacatgcg gcggtgttgt actcgctgat cggcacatgc
     2701 cgtctgaaca atgtggagcc agaaaagtgg ctgcgctacg tcattgaaca tatccaggac
     2761 tggccggcaa accgggtacg cgatctgttg ccctggaaag ttgatctgag ctctcagtaa
     2821 atatcaatac ggttctgacg agccgcttac tcttcaggcg atttacatga ccttcgacaa
     2881 caccgttact ccagcggctg ctggttgcct cacatatagc cgcagcatca gcttccatcc
     2941 ccgcagccac gcgccggagt tctgctgaac cgttttcgtg gactcgagtg aaccaactgc
     3001 ttagatttga tttatttggg gttttcagaa tacggtagaa ctcaagtact agttgctgcg
     3061 ctattttcag cgccggttct ttttcacaca tcagactaat aaatcgggaa gcataatttt
     3121 cttcacccct gattattatc cagggcatca accagcggct tacccgggac actgaaggaa
     3181 gtctggcggc agtagtaact ggtgggttcc agcctttacg ccatttggca acagcatccc
     3241 tgacgatggt ttcactgcct gtaacccccg ggccaccatt tcccgccata tccagtcgct
     3301 ggtgtgctga attggtcatg tatgaaggaa gatatcaatg gtggtatctc cctttcaaaa
     3361 ggaagtgaaa agcttgatgc ctgctgccag gcaatcaata ttacgtgctt ttaacgacta
     3421 tcctaaatgg tggtagctat gcagagctcc cggtcctagc gctgggagct tatcgcttat
     3481 ctttaccgta agcgtaaacg gaccgccgta tgtagccatt aaaccagtat tggtaacgta
     3541 gatacccacc ttttcaaaag ggaagtggat aaaagatagc gaaaatgcga agagttgatt
     3601 aatgcattga aacaaaattc attaatgatg aacttggaga aaagctccat cgttttattg
     3661 gctcaaaaaa catatcccat agatgatcag ccataccttc attggttagc aaggtatcct
     3721 gtgagtagcc atggctgaag atgcccattg atgggcgaaa accaaggatc tgaccgttag
     3781 agcagttatg tcgttaaaca catcaccacc atatgccatc cggctcactt cgtcggccaa
     3841 attagtgtga tattctttcc agaaatgctg ttgtatccac gacataaggc gatgattgat
     3901 ggagcagtct ctcaattttg aaatgttgcg cagccagtgg ccggaactgg ctgaactcgc
     3961 gtgtatggcg gaacgctatg ttcactccga tccggaaagt tgtctggtca agctgcgcaa
     4021 ctacaccgaa ttgatggtgc gctggttgta tcgtcaggag cggttgccgg aaggtattaa
     4081 ggctaatctt tacgatttaa tgaatgctga tgtctttacc agcatgatgc cggaagccat
     4141 catcatgaaa atggatgcgc tgcgtatcca tggcaaccgt gccgcgcacg gcggacgtat
     4201 caaagctaaa gatacttact ggctgctcaa agaagcgtat ttgttgggaa tttggctgta
     4261 tgttcgctac gcccacggta atgttgatga ctgccccaaa tttacactcc ctccattaac
     4321 acaatcttca ggtcgtgcag atgaaaaacg tctggaagat gcaatcaggg ctcaggatga
     4381 aagccgtgag cgcgaactgg cgctgcaacg cgcactacag caagaacagg aaaaggccga
     4441 acacctcact caacgcctga atgaagccag agcgcgtaac cagcatgttg ccgatattct
     4501 ctctattgat gaagcggaaa cccgccgtcg cctgattgac tctcgcctgc ttgctgctga
     4561 ctggaatgta ggcgaaggcc ttaaaaatac cgatcaggtt acgcaggaac atcccgttaa
     4621 agaacaacct accgcgaccg gagacggtta tgcggactat gtcttgtggg atgaggcaca
     4681 caaaccgctg gcggtggtgg aagcaaaaaa aaccagcgtc aatgccgagc aaggacgaat
     4741 tcaggcccgg ctgtatgcag actggctgga aaaagaatac gaccagcgtc cgatcatctt
     4801 ctacaccaac ggctatgata tctggctgtg ggatgaccat aaaactcatg gttatccccc
     4861 gcgtcgggtg tttggcttct acagcaagga aagcctgcaa tatcttattc aacagcgtga
     4921 aacccgtctt ccgctgaaca gtgtgccgca cgtaaaagat aacgaaggta aggccgttgc
     4981 cggacgtttg taccagcttg aaaccattgc ccgtgtcagc gagcggttta ccaacaaata
     5041 ccggcaatcg ctaattgttc aggctacggg tacaggcaaa acccgcgtgg caatagcgct
     5101 aagcaaacta atgattgatg cccgctgggt aaaacgcgtc ctgtttcttt gcgaccgcaa
     5161 ggaactacgt aaacaggcgg cgaatgcctt taatcaattc accaatgagc cgctgtatgt
     5221 ggtcgggaaa tcgaaaaaag cagacaggca gaatgctagg atctacatcg ccacctatcc
     5281 tggtatgatg aaaatcatgg accatttcga tgttggttat ttcgatttga tcatcgccga
     5341 tgaatcccat cgctctatct acaacgttta cggcgatctg tttaagtatt ttgatgccct
     5401 ccagattggc ctgacggcca cgccgattga catggtgagt aaaaccactt tcggcctgtt
     5461 tggttgtgaa ggacgtattc ctaccgccaa ctacagcctg gaagatgcca tcgccgataa
     5521 caatctggtt ccctatgagg ttgtcacgca caccacagga ttcctgcgcg aaggcatcaa
     5581 acgggagaaa ttaagcgacg cacaaatccg cgagctggaa gaacaaggca tagatcccaa
     5641 tacgctggag tttgatggca aggcgctgga tgaggcgatc tacaacaaag acaccaaccg
     5701 ctatatcctg cgtaacctga tggaaaatgg cctgaaagat cgggacggcc agttgccagg
     5761 taaaaccatc attttcgccc gtaaccacaa acacgcgctg ctgttgaatg aattattcga
     5821 cgatatgtac ccgcagtttg ccgggcgctt ctgccaggtc atcgacaatt acgatccccg
     5881 cgccgagcaa ctgattgatg attttaaagg gctggatgaa agcaccaaca aagagctgac
     5941 catcgctatc tccgttgaca tgctcgatac cggtattgat gtcccggaaa ttgtgaatct
     6001 ggtctttgcc aaaccggtca aatcaaaagt gaagttctgg cagatgattg gccgtggtac
     6061 gcgcctctgt ccggggttgt acggttacga cgataacggc aaacctctgg ataaacagaa
     6121 attccgcatt ttcgatcact ggggcaattt tgagtatcac gaactgcata ccgaagaggc
     6181 cgaagtcaca gcgacaaaat cgcttgcaca aaaacgcttc gaagcgtggg ttatgctggg
     6241 ggccgctgcg caacgtaaat tcgacaaaca ggcggtggat ttagttgctc accagctacg
     6301 cgagcaaatc aatgcactgg atgaaaagtc gattgccgtc caggaaaagt ggcatcaaaa
     6361 agcgcagtac agcgatgaaa aagtgctgcg ccagctttcc ccgaaaacac agcaggattt
     6421 gctatctgtc ctggccccgc tgatgcagtg gctggacgtg cgcgggcaaa gcgacgctat
     6481 gcgctttgat atggatattc tggcggcgca aactgcccgt tataccaacc cggaagagct
     6541 ggatgtgctc tggccggtca tcgtcgaaaa agtggagcgc ctgccgccgc atttggcgca
     6601 ggtccaacag caaggaccgc ggatcaatca gcttcgtgat ttaggctggt ggaagcaggc
     6661 cagcctggaa gagctggaag atatccgcat tcatctgcgc ggcatcatgc acctgatgga
     6721 aaaagacgcg acgccgaaat ttggttcgat acaggtggat attaccgaag atgcgaacct
     6781 gattcagacg gaaaaccgca aaaccaacat ccgctcgatt gacttcaaac tctatcgcca
     6841 gcaggtgcag ggagcgctgg agccgctgtt ccagcaaaat ccggtgctga agaaaatccg
     6901 caacggcgag cctgtgacgc aaagcgagct ggatgaactg gctaaactgg tgctgatcca
     6961 gaaccctaac gttgatatcc gggcactgaa agagttctac ccgcaggcga ccgccagcct
     7021 ggataaacta ttgcgtacca tcatcgggat ggacagcgac gcggtggaag tgcgctttgc
     7081 tcagttcgcc gctgataaca gcctgaccag ccagcaattg cgcttcctgt cattgctgaa
     7141 aaaccacatt cgcgattacg gcaccattga aatgcggcag ctctttgaac agccttttac
     7201 gcatatccac aacgaaggcg ttaccggtgt gttccctgat atagagcaga tcgcccgcct
     7261 acaaaagata gtcgaagagc tgggtgttgt gaccgacgca gcaacggtat aatacgcaca
     7321 tgacaagccc cggcagcggg gcttttgtat ttaaaacagg atataaaagc taacaatgat
     7381 cactggtgac ttaaaaagta aaatcgacgg cctgtgggaa gatttctggg tgggtggtat
     7441 caccaacccg ctgaccgtaa tcgaacagat cacctatcta atgtattccc ggatgctgga
     7501 tactcaggaa caacgcgacg agaagcgtaa acaaatcgcg ggtattgatt ttaaaccgcg
     7561 ttttgcacca gaacagcagg agttccgttt cagtcactat agcaatcttg gctcggatga
     7621 gatgatggaa gtggtgcgcg atggtgtatt ccagcatttt cgtcagctcg gccaggctga
     7681 tgcgtcgaag gtaacgctgc tgggcaactt tatgaaagat gcccgtctgg agattgttaa
     7741 gccttcattg ctgaccaaag cggttgaggt gatcaaaaac ctgccattgg atcgcggcga
     7801 caccaaaggc gacctttacg aatacctgtt aagcaagctg accactgccg gaatcaacgg
     7861 ccagtttcgc acaccgcgcc acattatccg cacgatggtt gaaatgatgg agccgaaccc
     7921 cgcccgcggc gagacgattt gcgatccggc ctgcggcacc ggtggctttc tggcaaccag
     7981 ctatgaatat ctactggaga agtacagctc gctggaatcc attcataccg agattggcac
     8041 caacgaacgt ggtgagctgg aagagcaaaa aatctttacc ggcgatctgt tgaccccgtg
     8101 gcgtaaccat gtggataaca atatgttcca cggttacgac tttgacacca caatgttacg
     8161 tatcgccgcc atgaacctga ttatgcacgg cgtggaagcg ccggatatcc actatcagga
     8221 caccatgagc cagagcttca gcacaaactt cccgcaggcc agtaaaaacg ctttcaacct
     8281 gattctggca aacccgccgt ttaccggttc actggacgaa gaagatatcg actccacgct
     8341 gtcggcaatg gtgaaaacca aaaaaaccga actactgttc ctggcgcgta ttctgcaaat
     8401 gctgaaagtg ggcgggcgca gtgccactat cgtgccgcag ggcgtgctgt ttggctccag
     8461 taaggcgcac cagtcacttc gcaaaacgct ggtggaagat aaccaactgg aagcggtgat
     8521 caacctgcct tccggcgtat ttaaacctta cgctggcgtg gcgacggcga tcctgatctt
     8581 taccaaaggc ggtcagacgg acgaggtctg gttctacgat ctgcaaaatg atggctacag
     8641 cctggatgac aagcgtaacc caattaaaga caacgacctg ccgcatctgc tggcaagctg
     8701 gaagcattac cgtactttac gcgggctgcc ggttgataac tttatgggtg agaagttagc
     8761 ctcgttgctg aaacagcagt acccggaagg gattgatgct ggcgttgatt ttaaagatcg
     8821 cactcaggcg gcgtttatag tgccaaaaga agatattgct gcccagaaat acgatctctc
     8881 tattaatcgt tataaagaag tggtgtatca ggctgaggag tatgaagatc cgaaggtgat
     8941 attgaagcgg ttaaaagatc tggaaaaaga gattctggcg gatctggatg agctggaggg
     9001 gatgctgtga aaaaaaatca attggtcaca cttggcaaac atatagacat attgtccggg
     9061 tgtgcattcc cttcaagtgg attcaatagg aacaatggtg ttccactaat ccgtattaga
     9121 gatattcttt caggaaaaac cgaaacttat tatgaaggca gttatgactt aaaatatctt
     9181 attaaaaaag gtgatttact ag
//
LOCUS       DAPHDX010000157         9048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPHDX010000157 DAPHDX010000000
VERSION     DAPHDX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.159"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..443
                     /locus_tag="RH058_003879"
     CDS             171..443
                     /locus_tag="RH058_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV3139398.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(444..1316)
                     /gene="rluF"
                     /locus_tag="RH058_003880"
     CDS             complement(444..1316)
                     /gene="rluF"
                     /locus_tag="RH058_003880"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV3139399.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1528..2217
                     /gene="pepE"
                     /locus_tag="RH058_003881"
     CDS             1528..2217
                     /gene="pepE"
                     /locus_tag="RH058_003881"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV3139400.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2308..3939)
                     /locus_tag="RH058_003882"
     CDS             complement(2308..3939)
                     /locus_tag="RH058_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV3139401.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4159..7842)
                     /gene="metH"
                     /locus_tag="RH058_003883"
     CDS             complement(4159..7842)
                     /gene="metH"
                     /locus_tag="RH058_003883"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV3139402.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            8042..8866
                     /gene="iclR"
                     /locus_tag="RH058_003884"
     CDS             8042..8866
                     /gene="iclR"
                     /locus_tag="RH058_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV3139403.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2102 a         2480 c         2324 g         2142 t
ORIGIN      
        1 taaattattt ttgtttgtag agggaaaact tttcgctaaa tttgtatctg tatttatcgt
       61 caattatcaa gaaagataaa tatttaagaa gagaacaaag gaatattatc agttcagctc
      121 gcaaacgaat tcccgtacac tgaactctct ggctcactga gggcaccatc atggcactcc
      181 cccgtattac ccaaaaagag atgactgaac gcgagcagcg tgaacttaaa acactgctgg
      241 atcgggcgcg gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga
      301 aagagtacat cgataaatta atggtcgagc gtgaggctga agcgaaaaaa gcccgccagt
      361 tgaagaaaaa gcaggcttat aaaccggatc ctgaagcgtc atttagctgg tcagcaaata
      421 cgtcgacgcg cggcaggcgc tgattagcgc cccttctttt tacgccccgg agaggtaaaa
      481 cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact
      541 ggacgtttga tgcccgctgt tttcggtttg gccttcactt taggttttgc ctcggaagag
      601 gaattttcga tgagcttaaa gaggtcgatt aactcatcat cagttaaatc gcgccactca
      661 cccagcggaa tgccgcttaa gctgacgttc ataatgcgcg tacgttccag ctttttcact
      721 tcatagccga aatgctcgca catgcgccgg atctgacggt tcagcccctg caccagggta
      781 atgcggaaga caaacggcgc ttcttttttg actttgcact ttttcgtcac cgtcccgagg
      841 atcggcaccc ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgtc
      901 accagatact ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg
      961 tgattggtga ggaaaatcag cccctgggag tctttatcca ggcggccaat cgggaacacg
     1021 cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg
     1081 acaataccga cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggctca
     1141 atcaactgac cgtttacttt cacaacgtcg ccgggtttca cctgatcgcc aatggtggct
     1201 cgcttgccat taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag
     1261 caaattccgc tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat
     1321 tctcctgtaa aaaacggaat ataccgtacc tttgggttga taaaaaatag tcatgagcga
     1381 actatttgtt atttccattg gcttgaattc gttacctgct caaatcactt tccagaagca
     1441 atgtgcgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta gcatgcctcc
     1501 cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa ctcgacgctg
     1561 ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt gcagggtcgc
     1621 cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga ttacacagcg
     1681 aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca tagcgttgtc
     1741 gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcggcggcgg gaatactttc
     1801 cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga cgtggttaaa
     1861 cgtggcgctc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aaccattcgt
     1921 accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa tctgttcccg
     1981 ttgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt
     2041 gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat tggtctaccg
     2101 gaaggtaact ggatcacagt gagtaaaggt cacgctacgc tgggtggccc gaacaccact
     2161 tatgtgttta aggctggtga agaagcggtg ccgctagaag ctggtcaccg tttttaagtg
     2221 aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg cttgacgcgt
     2281 cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat cttcatccgg
     2341 ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcaggcgct gcatatctcc
     2401 cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct gatgcaggcg
     2461 atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aagcgatgct tactacggcg
     2521 caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca gttttaaatt
     2581 actgagcaat tgctcataga gcgcatccag ttccttcaag ccatcaagcg aaaatgcccg
     2641 ccgtgctgcc agcgatttat cggcaatttc gctgcccatg cgctcgacga tatcggaagc
     2701 ctgttcaagg ttgagcgaca tttcaataat ctccgcccag cgacgcgact cttcttctgc
     2761 cagttcctct tttggcatcc gcgccagata cagcttaatg gcggtataga gaacgttgat
     2821 atcatctgcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca tcactttatt
     2881 cagcccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc gcgcggcgtt
     2941 cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg
     3001 ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac
     3061 aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat agatcaccag
     3121 ttccgctttt ggcaatgaca acttccccat tgtctctgcc agcaaatgga caaacggcag
     3181 gataatcagg ctacccacca gcttaaacag caaactaccc agcgcgacac ggcgggctgc
     3241 ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt tagcaccaat
     3301 caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag tcgcagtcag
     3361 cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa tcagcgcatc
     3421 cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg cctgggtgat
     3481 cggcgttacg gcctgcacaa tcaactccag cgccagcaaa atcagcccaa gaccaatacc
     3541 gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat
     3601 aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg ccattagcgc
     3661 cgtcccgaca tctgcaccca gcacaatgac cagagccggt gcgagggcta ccagatcctg
     3721 agcgacaaac gaggtcacca gcatggtggt ggcattactg ctctgcacca gtgcggtaac
     3781 gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg
     3841 caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc cccagaccag
     3901 cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt ctccttatcg
     3961 ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga ctggcatctt
     4021 tactgtgcct tttgacattc gtttttccgg ctaagattat tccagcctgt ttccagtata
     4081 agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg ttgtaaggaa
     4141 aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca gccagcgctc
     4201 aacatcggag acgctcatac ctttacggcg ggcatagtct tcaacctgat cgcgctgaat
     4261 ttgcgccacc gcgtagtact tgctgtccgg gtggctgaaa taccaacccg aaaccgatgc
     4321 accgggccac atggcgaaag attctgtgag tttcatgccg gtatgttttt ccacttccag
     4381 cagctcccag atggtggctt tttccgtatg ttccgggcag gccgggtagc ccggtgccgg
     4441 acggatgccc tggtagtttt cgcggatcag ttcttcgttg ctgaggttct cgttcggcgc
     4501 atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg cttcggctaa
     4561 acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc
     4621 atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga tgtaatctgc
     4681 tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga atcccgtttt
     4741 ttcggtctgt tgacgcagat ggtggctgac gttgatcaca tgggtgcgcg tttcgtcacg
     4801 gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca cgccacgcgg
     4861 attcagcgtt ttctcggcgc ttaatttatc cagcatgtcg ttggcgtctt taaacagccg
     4921 ctgcgcctca acgcccacca cttcatcttc cagaatgcgc ggatacttcc cggccagcga
     4981 ccaggtcata aagaacggtg tccagtcgat gtaattacgc agcgtttcga tgctggcttc
     5041 aacttcctgc acgccgagac ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc
     5101 gaaatcgtta tcgcgcgccg cttccagcgt gaccggtggt gtgcgcggtt tcttacgccc
     5161 gtgctgaata cgtacggttt cgtactcctt gcgggtacga gcgacaaaat catcacgctg
     5221 agtatcggaa agcagcgccg ccaccacacc aacggtgcgc gatgcgttct gtacatacac
     5281 cgtcgggccg ctgtagttct gctcaatttt caccgccgtg tgcgcttttg aggtcgtcgc
     5341 gccgccaatc aatagcggga tggtgaagcc ctgacgctcc atctctttcg ccacgttaac
     5401 catctcgtcc agcgacggcg tgataagccc cgaaagtcca atcagatcag cattcacttc
     5461 tttagcggta cggagaattt tttccgcagg caccataacg ccgagatcga caatttcgta
     5521 gttgttacat tgcagcacca caccaacgat atttttaccg atgtcgtgga cgtcgccctt
     5581 cacggtggcg atcaccatct tgccgttggt tttgccctgc tctttgctgg cttcaataaa
     5641 cggttcgaga tacgccaccg cctgtttcat gacgcgcgcc gatttgacca cctgtggcag
     5701 gaacattttc ccttcgccaa acagatcgcc gaccacgttc atgccgtcca tcaacgggcc
     5761 ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct tcttcggtat cctgctcgat
     5821 aaactcggta atgcctttaa ccagcgagta ttccagacgt ttattcactt cccacgagcg
     5881 ccactccgcc tgctgggcgt tagcggtgtc gtcggttttg ctgccgcgat atttctcggc
     5941 aagctccagt aaacgctcag tgccatcgtc gcgacggtta agaatcacat cttccaccgc
     6001 gtcgcgcagt tcagcgggca ggtcgtcgta aatcgccagt tgcccggcgt tgacgatccc
     6061 catatccatg ccattgcgaa tagcgtagta gaggaacacc gcgtgaatgg cttcgcgcac
     6121 cggatcgttg ccacggaatg agaaagaaac gttagataca ccgccggaaa tcagcgcgtg
     6181 cggcagttcg cgtttgatat cttcacacgc gccgataaag tcctgcgcgt agttgttgtg
     6241 ctcttcaatg ccggttgcga ccgcgaagat gttcgggtcg aagatgatat cttctggcgg
     6301 gaagccaacc tcttcggtga ggattttgta cgcccgacgg caaatctcga ttttccgtgc
     6361 gcgggtatcg gcctgtccct gttcgtcaaa ggccattacc accactgccg caccgtaacg
     6421 acgcaagagc ttcgcgtgat ggataaaggc gtcgacgccc tctttcatcg agatagagtt
     6481 aacaatgcct ttgccctgga tacacttcag acctttttca atgacatccc attttgagga
     6541 gtcgatcata atcggcacgc gagcgatatc cggttcaccg gcaatcagat tgagaaaacg
     6601 caccatcgcc gcttcggcgt cgagcatccc ttcatccatg ttgatatcga taatctgcgc
     6661 accgttttcc acctgctggc gggccacatc cagtgcctcg ctgtatttct cttctttgat
     6721 caggcgcttg aacttagcgg aaccggtgac gttggtgcgt tcacccacgt tcacaaacag
     6781 gctatcttcg ccaatgttca gcggctccag gccggacaaa cggcaggcta cggggatttc
     6841 cggcagtttg cgcggcgcta atccttctac tgcacgactc atcgctgcaa tatgttgtgg
     6901 cgtggtgcca cagcagccgc cgacgatatt gagaaaaccc gcttgcgccc attcacgtat
     6961 ctgttttgcc atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg gtagcccggc
     7021 gttcgggtgc gcggtgacgt agcattccgc aatccgtgac agctcctgca cgtactggcg
     7081 cagttcatcg ggccccagcg cacagttcag gccaaaggtc agagcttcgg cgtggcgcaa
     7141 tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg cgcccggagg cgtcggtgat
     7201 ggtgccggag atcataatcg gcagctcaac gcccagcgct tcaaactccg ttttcaccgc
     7261 aaaaaccgcc gctttggcgt taagggtgtc gaaaacggtt tcaatcagga tcagatccgc
     7321 accaccttcc accagcgctt tggtggattc gcgataggcc gccaccagct ggtcaaaagt
     7381 gatgttgcgg aatgccggat cgttgacgtc cggagaaata gacgccgtgc ggttggttgg
     7441 gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg cgcgcggtcc actcgtcagc
     7501 acaggcgcgc gccagtttcg ccgccgcaaa gttgatttcc gccgacaggg attccatctg
     7561 gtaatccgcc atcgcaatgg tcgtggagtt gaaggtgttg gtttcgatga tatccgcgcc
     7621 cgcttcaaag taggcgttgt ggatagcggc gatcacttcc ggtttgctga gcaccagcag
     7681 gtcgttgttg cctttgagat cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc
     7741 ttcgttcagt cgatagctct ggatcatggt gcccataccg ccgtccagca ccagaatacg
     7801 ttcatttaac tgcgcacgca gttgttccac tttgctgctc acacttgctc ccgacacgct
     7861 caacccagat ttaataaaaa ttcaacaaac catactggca taaacgcatc tgtggtaaaa
     7921 gcgaccacca cgcaacatga gatttgttca acattaactc atcggatcag ttcagcaact
     7981 attgcattag ctaacaataa aaatgaaaat gatttccacg atacagaaaa aggaggctgt
     8041 catggtcgca cccattcccg cgaaacgcgg cagaaaaccc gccgttgcca ccgcaccagc
     8101 gactggacag gttcagtctt taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc
     8161 caatggcagt gtggcactca cagagctggc gcaacaagcc gggttaccca attccacgac
     8221 ccaccgcctg ctaaccacga tgcaacagca gggtttcgtg cgtcaggtcg gcgaactggg
     8281 acattgggca atcggcgcac atgcctttat ggtcggcagc agctttctcc agagccgtaa
     8341 tttgttagcg attgttcacc ctatcctgcg caatttaatg gaagagtctg gcgaaacggt
     8401 caatatggcg gtgcttgatc aaagcgatca cgaagcgatt attatcgacc aggtacagtg
     8461 tacgcatctg atgcgaatgt ccgcgcctat cggcggtaaa ttgccgatgc acgcttccgg
     8521 tgcgggtaaa gcctttttag cccaactgag cgaagaacag gtgacgaagc tgctgcaccg
     8581 caaagggtta catgcctata cccacgcaac gctggtgtct cctgtgcatt taaaagaaga
     8641 tctcgcccaa acgcgcaaac ggggttattc atttgacgat gaggaacatg cactggggct
     8701 acgttgcctt gcagcgtgta ttttcgatga gcaccgcgaa ccgtttgccg caatttcaat
     8761 ttccggaccg atttcacgta ttaccgatga ccgcgtgacc gagtttggcg cgatggtgat
     8821 taaagcggcg aaggaagtga cgctggcgta cggtggaatg cgctgacttt ttctggcggg
     8881 cagaggcaat attctgccca tcatacctga gtggcaatag aataagggtg tctgttaatc
     8941 gcattgacgc caaaataact taatgtcata cacttcacac acttttatta actcagcatt
     9001 atttttaaac atcaaactac ttaatcatta acaaaatcgc aagatctc
//
LOCUS       DAPHDX010000158         9015 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPHDX010000158 DAPHDX010000000
VERSION     DAPHDX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.160"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            14..1426
                     /locus_tag="RH058_003885"
                     /pseudo
     CDS             14..1426
                     /locus_tag="RH058_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(1893..2879)
                     /locus_tag="RH058_003886"
     CDS             complement(1893..2879)
                     /locus_tag="RH058_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139404.1"
                     /translation="MTMTKDAFQFGIEPVRITDTDNIQINEGLPTNADPQVYALELAK
                     LVKTMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGTEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFSCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTETN"
     gene            complement(2963..3454)
                     /locus_tag="RH058_003887"
     CDS             complement(2963..3454)
                     /locus_tag="RH058_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139405.1"
                     /translation="MLFNNNNWKLSVTDINLYENTVSLDDQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQLNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSKASIMADLDSGANWM
                     DCL"
     gene            complement(4074..4784)
                     /locus_tag="RH058_003888"
     CDS             complement(4074..4784)
                     /locus_tag="RH058_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139406.1"
                     /translation="MSQQVVIFNDEFSLSSYEFLTKVINPAREEAGENPVSNKDFINR
                     VKDELDLKEENFLLLDTGASGRKSSHTILNGDQLLLVGMRESKAVRRKVLEYIRRIEK
                     DKQLLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKNPISNEW
                     SVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGRPETIDKMKGILIAFTGTDQ
                     QKQEYLLKQAKSGKQKGL"
     gene            complement(5552..6301)
                     /locus_tag="RH058_003889"
     CDS             complement(5552..6301)
                     /locus_tag="RH058_003889"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3139407.1"
                     /translation="MDHYCTVRDTKPLDRIKLYDHYTFDPTIPQLLRVKTPYGQSKRA
                     TGEPVSFYVHQNGYASVSVLGKRYRRSRVIYSLCKGHIPEGMFVDHIDGNRLNDHPDN
                     LRLVTPQQNQWNRDNLGGKLQNYLPKGIDFVKAEGRYRARIKIDGKVRARLSYEVREL
                     MVWLQEQRQKLHGEFAYDGWTDWTEADQKRADERYEVSKPEGYKPRKRRLPGYAKTHD
                     GLKVYIEPKTGRLYFKKLNKKHYIDEESVVV"
     gene            6360..7382
                     /gene="istA"
                     /locus_tag="RH058_003890"
     CDS             6360..7382
                     /gene="istA"
                     /locus_tag="RH058_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HDV3139408.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            7382..8161
                     /gene="istB"
                     /locus_tag="RH058_003891"
     CDS             7382..8161
                     /gene="istB"
                     /locus_tag="RH058_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDV3139409.1"
                     /translation="MMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(8227..8916)
                     /locus_tag="RH058_003892"
                     /pseudo
     CDS             complement(8227..8916)
                     /locus_tag="RH058_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
BASE COUNT         2212 a         2283 c         2353 g         2167 t
ORIGIN      
        1 cagggagtga gttatgtcca aaaatgaccg aatggtgggc atcagccgca gaacactcgt
       61 taaatcaacc gcgataggtt ctctggcgct ggctgcaggc ggtttttctt taccgttcac
      121 cctgcgcagt gcagcggcag cagtacaaca ggccagcgaa aaagtgatct ggggtgcctg
      181 ttccgtcaac tgtggtagcc gctgtgcact tcgtctacat gttaaagata atgaagtcac
      241 ctgggtggaa actgacaaca ccggcagcga cgagtatggc aatcatcaag tacgcgcctg
      301 tttgcgtggt cgatccatcc gccggcgtat taatcatccc gatcgcttga attacccaat
      361 gaaacgcgtg ggtacacgcg gtgaaggcaa attcgaacgg attagctggg atgaagccct
      421 ggatactatc gccagtagcc tgaaaaaaac cgtcgaacaa tatggcaatg aggctgtata
      481 tattcagtac tcttcgggga ttgttggcgg caatatgacc cgctcttcgc catcagcctc
      541 ggcggtcaaa cgcctgatga actgctacgg cggttcactc aatcagtatg gctcctacag
      601 caccgcgcaa atttcctgtg ccatgcccta cacctacggc agtaatgatg gcaacagcac
      661 cacggatatt gaaaacagca agctggtcgt gatgtttggt aacaacccgg cagaaacccg
      721 catgagcggc ggtggcatca cttatcttct tgaaaaagcg cgcgagaaat cgaacgccaa
      781 aatgattgtt atcgatccgc gatataccga tacggctgct ggtcgtgaag atgaatggct
      841 ccctattcgc ccgggcaccg atgccgcgct ggttgcgggt attgcctggg tattgattaa
      901 cgaaaatctc gttgatcaac cttttctcga taaatactgc gtcggctatg acgaaaaaac
      961 cttacccgca gatgcaccca aaaatggtca ctataaagcc tatattcttg gtgaaggtga
     1021 tgataacaca gcgaaaacgc cgcagtgggc ttcgcaaatt accggtatcc cggtagaccg
     1081 tatcatcaaa ctggcgcgtg aaattggcac agcaaaaccc gcttatatct gccagggctg
     1141 ggggccgcaa cgccaggcaa acggcgaact gactgcacgc gctattgcta tgttacctat
     1201 tttgacgggc aatgtcggca tcagcggcgg aaatagtggc gcgcgtgaat cgacctatac
     1261 cattaccata gaacgcctgc cggtgctgga taatccggtc aaaacgtcaa tctcctgctt
     1321 cagctggaca gatgctatcg atcatggtcc gcaaatgacg gcaatccgcg acggcgtccg
     1381 cggcaaagat aaactggatg tgcccattaa gttcatctgg aacttcaagg acttgaagcg
     1441 gacccttagt aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca
     1501 gacttgtgtc attagacggg atgtctagac ggctggtcag gaccctccgg tggccttggt
     1561 atggacttga ggcgggacta tgggggaagt ctgctggctt aatgtattag atggtcattc
     1621 acatttttct gtcagaaacc atcggtccca ctgtgagtcc aaacctaagg acctagaagt
     1681 ccacccacag ccttagacag agtgtcgaac acatccacac cgagtcgacc agagaggacc
     1741 atgtagagac ccacagagag gaacacgagt ttatagacca gagtcaggag ggacttcagg
     1801 tctgcggaag tcatagacat ttggccctcc ttatcttgta tactaccata ccagaaataa
     1861 ggcccagacc cgaaggtcca gaccatagtt agttagttgg tctcagtaaa gctcaggcgt
     1921 ttgactacag gcaggacagc agtagcacta ccagtaccca tcttgattgt cgtagcagct
     1981 tcagcgacca cgaagacctt gccgaggatt tctaggtcgt gagactgaga gtcgccatct
     2041 ctggataaga ccacggtaat gttctcacca tcatgacgga agcagctgaa gctcagggcc
     2101 gtaaaacgct tagccatcat gaacacacca cggtcctcac cgttgtaata cgcgatgtcg
     2161 gtacccagca tatccacgat agcgtcttcg ccaccgaagc cctgcttagc agccagacgg
     2221 gagagagcct tgaggtgtga ctcatgggcg ataatgccgt agtcagaaac ctcagtaccg
     2281 aaagaagact cggtgttctc atacagggca tccatgatag cgtcagcctt agcggtcagg
     2341 ctaccagtca ggaccggaag ctctacagtc ttcaacagct tggaggattc cagtacatcg
     2401 accatgcgac tattgatggt acggaccacg ttatcgcaga tgttcagagc gtcctgctgg
     2461 agcgtcccct gagtgaaacc agtggaagac ttagcgtccc acagcttggt ttgatgtgat
     2521 acgaggatag cctgttcaat cggctccacg gtgatttcag tgccggacgg agcggtcacg
     2581 acttcaggtc gcttgccacc acggacaggg accagcacag agcggtcagc cagagtgtga
     2641 gcaatgattg gagtcggtaa gctgttacgg gtcggcagga cttctacagg gaacgggatg
     2701 ttatcttgag cgtctttaag cacaccattg agcatcgtct taaccagctt agccagctca
     2761 agggcgtaga cctgagggtc tgcgttagtt ggcaaacctt cgttaatctg aatgttgtcg
     2821 gtgtcggtga tacgtactgg ctcaatgccg aactggaaag cgtctttagt catagtcatg
     2881 ataattaatc tccttgagtc aaatacttga agtccctaac ggtaagtcag ggaccgtgtg
     2941 ttcttagatg agggtcgctt gcttacaagc agtccatcca gttagcgcca ctatcgaggt
     3001 cagccataat tgaagccttg gaacgaccag cggaatgggt agcactgcgg aggttctcac
     3061 cataggtccg gcgttcagct ttgacctgtt cacggcgctg tagctctttc tgccactctg
     3121 catcggtcat gttctcttca cgcatacgct cagcctgaag tcgttgctct ttagcgatac
     3181 gctcagcctc agctcgtttg ttaagctggc ggcgaccata ggcggtcatc aggtcattac
     3241 tgaagaagtt accaatggtc acgccagctt cagcaagagc ttccagtaac tccattgctt
     3301 cgcggctggt ggatggcaag cctgacagat accccggaat gagggtcttg atagcgaggg
     3361 acagcggata ggactgatcg tccagactca cagtattctc gtagagattg atgtcggtga
     3421 ctgacagctt ccagttgttg ttgttgaata acataagttg gtactcctct tgagaaggtg
     3481 agaactatcg gtatcgcaga cacagttatg cctacgggtc tccccgcatc ggtcattctc
     3541 aaaaggtgtt taaattaagg tattgagtag gtgagctgct aacggttagg agacagctag
     3601 cttactagcg acagtttcat agcgaccagt tgcgctatca gctcgtctcg ggattggtgc
     3661 tggcgggtct catcttccgg gtctagacca agctccttca ggaggaattc agtccgatag
     3721 ccacggtaac ggagagtcat gcgaccctca ggtgtgcgcg ggtcaaccat aggtctttgg
     3781 gagaccatag gtctttggtt agtcatcatt gttagaagtc ctcgttagac caatcatcca
     3841 cgtcaaattc actggtgata ggcgcttgga agtcaggtag accctctggt atatctagcg
     3901 gcagtcgctc gtcgactctc agtgccatct cttcgtatat atgccgaagc agagaagtca
     3961 gtaagatatg gtccgcagga tgctcatggt cgtatctctc aatgaaacat atggttgtcg
     4021 ctattgactt cataatcagt tcatcagtaa gtcggttgga atccatagtc atcttagagt
     4081 cccttctgct tgccggattt ggcttgcttc aggaggtatt cctgcttctg ctggtcggtg
     4141 ccagtgaatg caataaggat gcctttcatc ttgtcaatcg tctcaggtct accgaagacc
     4201 tccagtccct tagcgttgaa gcgaaagtta taaccacctg ctgagaactt ctggtctgac
     4261 ggcttaatga tacggaagca gtcggaatac tttggattta cagaccactc gttactgatt
     4321 ggattcttac gaaagagacc acgatgctga agaagtgcgg tcattagctt ctgagcaggt
     4381 agaccatagg ttttgcagta gtcaccccaa gtcacgcctc gacggttagc ggactgaatt
     4441 gctgctcgct tcttctggtc ttccagtaac tgcttgtcct tctcaattcg tcggatgtat
     4501 tcaaggactt tgcgacgcac agctttagac tcacgcatac cgaccaatag tagttggtct
     4561 ccgttaagga tagtgtgaga ggatttacgt ccagaagcgc ccgtgtctaa taaaaggaaa
     4621 ttttcctctt ttaaatccag ctcatccttc acgcgattga tgaagtcctt attactgacg
     4681 ggattctccc cggcctcttc acgggcagga ttgataacct tggtcaggaa ctcataggaa
     4741 gacagagaga actcatcgtt aaagatgact acttgttgag acatatcgtt gtactccttt
     4801 ttgttatgcc tcctgaagtc acaccctgag agcctactca agcccccaac gatagggaga
     4861 ctcacaggca acaggagaca tacagaaaga cttgaggtga tgatgaggtc gtgtgaagac
     4921 cagagatgat ggtggtggtg gtgactgctc agttttgagc gcgttaagtt cttcgaccat
     4981 agagacgaag gttgcaagac cgcatgtcag gacgagctgt aaggactcca gatggtgagc
     5041 ggtgatagac ttaagccaat gtagtgagta gacgagctac taactgagat gactgaagaa
     5101 cgctaacgag ctggaggtcg atacacgagg tctgacagag gcggtcttcc taccagtgat
     5161 agttaagccc taccactggt catacatacc caacctcaag caacgaccca atgtatacag
     5221 ggtcccgatg ctttgagctg gagactgacg gagctatcgt cgaccccatg gttgaggaag
     5281 acacatagaa ggaatgaggt ctgacttaag tctggactat aggtcttata ttaatcttca
     5341 taagaagata ataattaact tagagtcata acttcagtct tacttagagt cttaacttta
     5401 agtcagcatc tatggtctgc cccccctccc cccattgctg gaaggagagg cggtagtgtc
     5461 tgccattttc cctatactga taatttagca ccctcctcta cagaccagac gcaccaaggc
     5521 ctacagaggc gctctaagtt agcaaccgtc atcaaaccac cacagactct tcgtcgatgt
     5581 agtgcttctt gttcagcttc ttaaagtaga gacgcccagt tttaggctca atgtagactt
     5641 tcagtccatc atgcgtctta gcgtagccgg gtaaacgtcg cttccttggt ttataccctt
     5701 caggcttgga cacctcgtag cgctcgtcag ctcgcttctg gtctgcctct gtccagtcgg
     5761 tccacccatc gtaggcgaat tcaccgtgta gcttctgacg ctgttcctgt agccagacca
     5821 taagctccct gacctcataa gagagtctcg cccggacctt gccgtctatc ttaatacgcg
     5881 ctctgtagcg accctcagct tttacaaagt caatcccttt cggcaaatag ttctgaagtt
     5941 tgcctccaag gttgtctctg ttccactggt tctgctgtgg tgtaactagg cgcaggttgt
     6001 caggatggtc attgagtcgg ttaccgtcga tatggtcaac gaacattccc tcagggatgt
     6061 gacccttaca caggctgtag atgactctgg agcgacgata gcgcttaccc agtacagaga
     6121 cagaggcata gccattttga tgcacataga agctaacagg ctctcctgtg gcgcgtttag
     6181 attggccgta aggtgtcttg actcggagta gctgaggaat ggttgggtca aaggtgtaat
     6241 ggtcgtacag tttgattcgg tctagtggct tggtgtcacg aacggtgcaa tagtgatcca
     6301 cacccaacgc ctgaaatcag atccaggggg taatctgctc tcctgattca ggagagctta
     6361 tggtcacttt tgagacagtt atggaaatta aaatcctgca caagcaggga atgagtagcc
     6421 gggcgattgc cagagaactg gggatctccc gcaatacggt taaacgttat ttgcaggcaa
     6481 aatctgagcc gccaaaatat acgccgcgac ctgctgttgc ttcactcctg gatgaatacc
     6541 gggattatat tcgtcaacgc atcgccgatg ctcatcctta caaaatcccg gcaacggtaa
     6601 tcgctcgaga gatcagagac cagggatatc gtggcggaat gaccattctc agggcattca
     6661 ttcgttctct ctcggttcct caggagcagg agcctgccgt tcggttcgaa actgaacccg
     6721 gacgacagat gcaggttgac tggggcacta tgcgtaatgg tcgctcaccg cttcacgtgt
     6781 tcgttgctgt tctcggatac agcagaatgc tgtacatcga attcactgac aatatgcgtt
     6841 atgacacgct ggagacctgc catcgtaatg cgttccgctt ctttggtggt gtgccgcgcg
     6901 aagtgttgta tgacaatatg aaaactgtgg ttctgcaacg tgacgcatat cagaccggtc
     6961 agcaccggtt ccatccttcg ttgtggcagt tcggcaagga gatgggcttc tctccccgac
     7021 tgtgtcgccc cttcagggca cagactaaag gtaaggtgga acggatggtg cagtacaccc
     7081 gtaacagttt ttacatccca ctaatgactc gcctgcgccc gatggggatc actgtcgatg
     7141 ttgaaacagc caaccgccac ggtctgcgct ggctgcacga tgtcgctaac caacgaaagc
     7201 atgaaacaat ccaggcacgt ccctgcgatc gctggctcga agagcagcag tccatgctgg
     7261 cactgcctcc ggagaaaaaa gagtatgacg tgcatcttga tgaaaatctg gtgaacttcg
     7321 acaaacaccc cctgcatcat ccactctcca tctacgactc attctgcaga ggagtggcgt
     7381 gatgatggaa ctgcaacatc aacgactgat ggtgctcgcc gggcagttgc aactggaaag
     7441 ccttataagc gcagcgcctg cgctgtcaca acaggcagta gaccaggaat ggagttatat
     7501 ggacttcctg gagcatctgc ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc
     7561 gatgtatacc cgaatggcag ccttcccggc ggtgaaaacg ttcgaagagt atgacttcac
     7621 attcgccacc ggagcaccgc agaagcaact ccagtcgtta cgctcactca gcttcataga
     7681 acgtaatgaa aatatcgtat tactggggcc atcaggtgtg gggaaaaccc atctggcaat
     7741 agcgatgggc tatgaagcag tccgtgcagg tatcaaagtt cgcttcacaa cagcagcaga
     7801 tctgttactt cagttatcta cggcacaacg tcagggccgt tataaaacga cgcttcagcg
     7861 tggagtaatg gccccccgcc tgctcatcat tgatgaaata ggctatctgc cgttcagtca
     7921 ggaagaagca aagctgttct tccaggtcat cgctaaacgt tacgaaaaga gcgcaatgat
     7981 cctgacatcc aatctgccgt tcgggcagtg ggatcaaacg ttcgccggtg atgcagcact
     8041 gacctcagcg atgctggacc gtatcttaca ccactcacat gtcgttcaaa tcaaaggaga
     8101 aagctatcga ctcagacaga aacgaaaggc cggggttata gcagaagcta atcctgagta
     8161 aaacggtgga tcaatattgg gccgttggtg gagatataag tggatcactt ttcatccgtc
     8221 gttgacaagc ggagacagca gccggcagca tgcatccccc cagcccgaca gcagtgaacg
     8281 cctcagctcc acaccttgcc ggccgtatat ttctgactgg cgatacagcg gggtgtgctc
     8341 tgcatacttc gaggtcagca ctcggaccag cagccccggt ccggcgatac cccgctcgat
     8401 gggccgcgaa ggtgcaggtg cctgcacgat ggcatcgcac tgagtacagg catgtttttc
     8461 ccgtaccgtc cggataaccc ggaaggcgct acgcatcaac tccagctgtt cggcagtatc
     8521 ctcgcccaga tagctcagtg aaccgccgca gttcgggcag cacggcgccg caggcaacag
     8581 tcgcttttcg tcacggggta gtgattcagg gaacggctta cgggtgcggg tctgacgcaa
     8641 cggacgctgt actgccgggt catacaccct accagtcagc gtatcgctct ctttctgaag
     8701 ccggttcaga tcggcttcca tttgtgcgat acggcgggag actttttcgg aacgactgcc
     8761 gaagttcatc cggcggagtt tatccagctg cgcctgcaga tggtctattt cgcgctcccg
     8821 gttgctcagc ttttcctgca gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt
     8881 cagcaggaag atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat
     8941 tctgtgacaa ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag
     9001 gctgtcgcca gtcga
//
LOCUS       DAPHDX010000159         8931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPHDX010000159 DAPHDX010000000
VERSION     DAPHDX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.161"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1129
                     /locus_tag="RH058_003893"
     CDS             <1..1129
                     /locus_tag="RH058_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV3139410.1"
                     /translation="LHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISY
                     EQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRL
                     ARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLALISPAGLNDLIYTLI
                     QTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHG
                     TTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNI
                     NEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAF
                     LGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1157..1468
                     /locus_tag="RH058_003894"
     CDS             1157..1468
                     /locus_tag="RH058_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV3139411.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1524..3197
                     /locus_tag="RH058_003895"
     CDS             1524..3197
                     /locus_tag="RH058_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV3139412.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSHES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3222..4661
                     /locus_tag="RH058_003896"
     CDS             3222..4661
                     /locus_tag="RH058_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV3139413.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4718..4936)
                     /gene="marB"
                     /locus_tag="RH058_003897"
     CDS             complement(4718..4936)
                     /gene="marB"
                     /locus_tag="RH058_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV3139414.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4968..5351)
                     /gene="marA"
                     /locus_tag="RH058_003898"
     CDS             complement(4968..5351)
                     /gene="marA"
                     /locus_tag="RH058_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV3139415.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5371..5805)
                     /gene="marR"
                     /locus_tag="RH058_003899"
     CDS             complement(5371..5805)
                     /gene="marR"
                     /locus_tag="RH058_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV3139416.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6017..6682
                     /gene="marC"
                     /locus_tag="RH058_003900"
     CDS             6017..6682
                     /gene="marC"
                     /locus_tag="RH058_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV3139417.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6707..7897)
                     /gene="ydeA"
                     /locus_tag="RH058_003901"
     CDS             complement(6707..7897)
                     /gene="ydeA"
                     /locus_tag="RH058_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV3139418.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8047..8931)
                     /gene="yneK"
                     /locus_tag="RH058_003902"
                     /pseudo
     CDS             complement(8047..8931)
                     /gene="yneK"
                     /locus_tag="RH058_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2448 a         1911 c         2135 g         2437 t
ORIGIN      
        1 actgcatctg aatagcattt tccccaacct tgaaaaagca caagctattt ttactccggt
       61 aatgaatggt tctgtaaata tcatgtcaat tatgattgct ttcctggtcg ccaggaatat
      121 ggcgattagc tatgagcaag atgatctttt atgcggatta acggcaatag gagcattttt
      181 tattgtttat acgccatatc agatgataga tgggcaagca ttcctgacga ccaaatatct
      241 cggcgcgcag gggttgtttg ttgctgttat cgttgcattg atcaccagtg aaatattttg
      301 tcgcttagct cgaaacccaa aaatcaccat aacgatgccg gcagctgtac cgcctgcggt
      361 agcgcgttca tttaaagttt tattgccaat attttttgtc atggtgttct tttccgcact
      421 taattattgc ctggcactga tatccccggc aggactaaac gatcttattt acacattaat
      481 ccagacgccg ctcaaacata tgggaacgaa tatctttgcg gtaattatcc tgggggctgt
      541 gggtaatttc ctgtgggtgc tggggatcca cggacctaat accacatcgg caattcgaga
      601 aaccgttttt tctgaggcta atctggagaa tctctcctgg gccgctcaac atggcactac
      661 ctggggcgcg ccatatccga ttacctggac ttctattaat gatgcattcg ccaactgcgg
      721 cggctcaggt atgacgttgg ggttattgtt ggctattttt atcgcttcta agcgtgcgga
      781 ataccgtgat ctggcaaaaa tgtcatttat ccccggtatt ttcaatatca atgaaccgat
      841 aatgtttggc cttcctattg tacttaaccc catcatgatg gtgccgttta ttatggttcc
      901 cattgttaac tgtgccattg gttacttctt tgtttcgatg gaaattattc caccggttgc
      961 ttatgccgtg ccctggacta cgcccggacc tttaattgct ttcctcggaa ccggggggaa
     1021 ctggctggct ttactggttg gttttttatg tttaggtgtg gcgacaatga tctatttacc
     1081 ttttgttatt gccgccaaca aagtcaataa catgacaact aacggataat tccatttacc
     1141 caggaaggag attgtgatgt ttgcagatga agaacttgtt atggagttac tggtcaacgc
     1201 ggggcaggca cgttctgacg cgatggaagc catacgttgt gccgggcaaa aagactggca
     1261 gggcgcaact cagctaatgg ccagttctga atctgcctgt cttcaggcac ataagattca
     1321 gaccgcatta attagtcagg atgaaggctg tggcaaaata aaagttaatt taattctgat
     1381 tcatgcacag gatcatttga tgaatgcaat attatgccag gatttagcca gggaaattat
     1441 ttcattaaga aaagaactcc atgcctgatt ttttcctcca gtgcgatagc tggaggaaat
     1501 tattatactc ttaaggataa gtcatgaata taaagacgct taacgtcagc cttttgtctt
     1561 tttctattat tacagcattg tttccattga acgcgatggc aacaaaatta accatagagc
     1621 agcgccttga attgcttgaa aatgaattgt cgcaaaataa acaagagctg aaagcaacac
     1681 agaatgaact gggagtatat aaatcccgac tttcgacctt acaaaaaagc atcacggaaa
     1741 acaaatataa atcagcctcg cttgctgaaa tatcagcgac atctcccgtc gctgataaca
     1801 tcaaaaatga aaacggtgaa cagaactcgt ttaccgcagc gcatactata aatggatcgc
     1861 agcaagttgc cgttattgaa agtaaaggcg acaaaaccac tatcgaaagc gtgaccctga
     1921 aagatatcag taaatatata aaggatgata ttgggttcag ctatcagggg tacttccgct
     1981 caggctgggg tactggtaat cacggctcac cacaaactta tgcagcgggt tctctgggac
     2041 gttttggtaa tgagatgagc ggttggtttg acctgacctt aaatcagcgt gtttataatc
     2101 aggacggtaa aacggcaaat gcggtcgtta cctatgatgg caacgtgggt gagcagtata
     2161 acgatgcctg gtttggtgac agtgccaatg aaaatatcat gcagttcagc gatatttacc
     2221 tgacaacgcg aggtttttta cccttcgcac cagaggcaga cttctgggtc ggcaaacata
     2281 aactcccgca atatgagatc cagatgctgg actggaaaac cttaaccacg gatgtcgccg
     2341 cgggtgtggg gattgaaaac tgggcacttg gtgtagggct gtttgatatg tccttaagcc
     2401 gtgatgatgt cgatgtttac tctcgtgatt tttcgcgtac cagccagatg aataccaatt
     2461 ctgtggatgt tcgttatcgc aatatcccgt tatgggatga tgcgacatta tcattaatgg
     2521 ctaaatattc cgcacctaat aaaacggatc aacaacaaga taacgaaaat gacaacagtt
     2581 attttgaaat gaaagatagc tggatgctga cttctgtttt acggcaaaac ctgcaacgcg
     2641 atacgtttaa tgaatttacg ttacaggttg ccaataattc ctatgccagc agttttgcca
     2701 gtttctcaga tgccagtaat acgatggcgc atggtcgcta ttactatggt gatcatacca
     2761 atgggatcgc ctggcgttta atctctcagg gcgagatgta tcttactgac aatattatta
     2821 tggctaacgc gcttgtctat tctcacggcg aagatgttta tagtcatgaa agtggtgctc
     2881 atagtgattt tgacagtatt cgcactgtaa taagaccggc ctggatctgg aatacatgga
     2941 atcagacggg gcttgaatta ggctggttta agcaacagaa caaaactcag cagggtgtaa
     3001 cactaaatga atcggcttat aaaacgacac tctggcatgc attgaaagtg ggtgaaagca
     3061 ttttaggttc acgaccagaa attcgcttct atggaacgta tatcaatatt ctggataacg
     3121 aattatcgaa ttttaagttt aatgagaaca gcaaagacga gtttatggcc ggcatccagg
     3181 cggaagtctg gtggtaatct attttagtta aggagttgtg tatgtcagga tttaaaaaag
     3241 gttttttatg gggcggtgcg gtagccgcgc atcagttgga aggtggctgg aatgaaggag
     3301 gaaaaggcat cagcatcgct gatgtgatga ctgctggcgc tcacggggtg ccgcgtgaag
     3361 tgacagaagg cgttatcgac gggcttaatt atcccaatca tgaagcaatt gatttttatc
     3421 atcgctataa aacagatatt cagttatttg ccgagatggg attcaaatgc tttcgaactt
     3481 ccattgcctg gacacgaatc tttccgcaag gtgacgaaca ggagccgaat gaagagggtt
     3541 tacaatttta tgatgatctg ttcgatgaat gccttaagca gggaatggaa cctgtggtga
     3601 cgctttcaca ttttgagatg ccttatcatc tggtgacaaa atatggtggc tggcgaaacc
     3661 gtaaactgat cgactttttt atccgcttcg catcaacggt cttcacgcgc tataaagaaa
     3721 aagtaaagta ctggatgacg tttaacgaaa tcaataatca ggtgaatttc agcgaaagcc
     3781 tgtgtccatt tactaattcc ggtatcttgt attcgccaga ggaagatatc aatgagcgcg
     3841 aacaaataat gtaccaggcg gtacattacg agttagttgc cagtgccctg gcggtacaga
     3901 ctggaaaatc gatcaatcct gaatttaaca ttggctgtat gatcgccatg tgtcccatct
     3961 atcctctgac gtgtgcaccc aacgatatga tgatggccac gaaagcaatg catcgtcgtt
     4021 actggtttac tgatgttcat gctcgtggat attatccaca acatatgctg aattactttg
     4081 ccaggaaagg attcaacctc gatatcacac cagaagataa cgcgattctt gccagtggtt
     4141 gtgtcgactt tatcggcttt agctactaca tgtcttttac gacacaattt tcgcccgata
     4201 acccgcaact ggattatgtt gaaccacgag atttggtcag caacccttat atcgatacat
     4261 ccgaatgggg atggcaaatt gatccggcag ggctacgtta ttcactcaac tggttctggg
     4321 atcatttcca gttgccgctg tttattgtcg aaaatggatt tggtgcggtt gaccagagac
     4381 aagctgacgg cacggtgaac gatcactatc gcattgatta ctttgcttcc catattcggg
     4441 aaatgaaaaa agccgtagtt gaagatggtg ttgacttaat tggctacacc ccgtggggct
     4501 gcattgacct ggtttctgcc ggaacagggg aaatgaaaaa gcgctacgga atgatttatg
     4561 tcgataaaga caacgaaggg aagggaacgc tggaacggat acgtaaagcg tcgttttact
     4621 ggtatcggga tctcatcgcc aacaatggcg aaaatatttg agacttgagg aagatattac
     4681 ggagatgtca ggctctgtga tcagagcctg acaataacta catagcgtgt tgattataat
     4741 agggcacgcc gagcgcatcc gacttatcac tgccagtacc catgtgattt aaatcaaacg
     4801 gtggttgttc ctgcgatggg ggaacaacca cgacattggc acaagaagta acaactggct
     4861 gcgtggtttg ttccgcaacg ccctgcgcgg aaaagagaat aagcgcagct gctattgcgg
     4921 atgaaagtgg tttcatgatt gcctcagtga cgttgtcacg ttatcaacta gctgttgtaa
     4981 tgatttaatg gatgtaaaaa gcgcgattcg ccctgcatat tggtcatccg gtatttatgc
     5041 ggcggaacat caaagtaatt tttgaaggtt cgggtcagag tttgttgcga ctcgaagcca
     5101 tatcgttctg ccagatagag tatcggctcg ttactttcct tcagcttttg cgcgatttcc
     5161 gtcatcttac ggctgcggat gtattggcct aatgaatgac cggtttcttt tttaaacatc
     5221 cgttgcaggt gccatttaga gtaacctgaa cgctctgaca ctttctccag tgacagtggc
     5281 gattccaggt tgtcctcgat ccagtccaaa atgctatgaa tggtaatagc gtcagtattg
     5341 cgtctggaca tcgtcatacc tcttttttgt ttacggcagg actttcttaa gcaaatgctc
     5401 aagtgttgcc acttcgtccg ccgtcaggtt ttttgttaat tcttgatgca ggtcctggcc
     5461 aactaattga tggcattgtt cgcatattgc cgcgccgctg gtggtaagtt ttaccagtac
     5521 gccgcgctta tcattcgggt tcggcaacct ttcaacccag cctttacaga ccaggcgatc
     5581 cagcatacgg gtcagtgctc caaggtcgac agacaacact tttttcagtt caaccggagt
     5641 aatacacgcc gcgcagcgga tagagcagag caccttaaac tgtgccgcgg taatatccag
     5701 cggagacaga tactcgttaa gcagacgatc tttcttctga ttaaccatat ggattaagcg
     5761 acccaatgga ataatttcat tgaacagatc gctggtactt ttcacattag ttgccctggc
     5821 aagtaattag ttgcagggga taatattgcc caggcaagta taagtcaaat aaatgaattg
     5881 accgatgcca cgttttgcta aatcggttca agttttggtc aatgcgatgc aaggttagct
     5941 aaagagtgac actttatcct tgtttacccc ttatactttt cgctgataac ccagatacac
     6001 aggataacaa ccactaatgt tagatttgtt taaagcaatt ggcctggggc tggtggtgtt
     6061 actgccgtta gctaacccat taacaactgt cgcgttattt cttggcctgg caggcaacat
     6121 gaacagtgcc gaacgtaatc gtcagtcgtt gatggcctcg gtgtacgtat ttgccatcat
     6181 gatggtggcg tattacgccg ggcaactggt gatggataca tttggcattt cgattcccgg
     6241 tctgcgaatt gcaggcggct taatcgtcgc gtttatcggt tttcggatgc tctttccgca
     6301 acagaaagcg attgattcac cggaggcgaa aagcaagtcg gaagagctgg aagatgaacc
     6361 cagtgccaat attgcctttg tgccgctagc aatgccaagt accgctggtc cgggaaccat
     6421 agcgatgatt attagctcgg cgtccacagt gcgtcagagt tcaaccttcg ctgattgggt
     6481 actgatggtt gcgccgccgc tgatcttttt cctggtggcg gttattttgt ggggaagcct
     6541 acgcagctcc ggcgcaatta tgcggctggt gggcaagggt ggcattgaag cgatctcccg
     6601 tctgatggga ttcctgctgg tatgtatggg tgtgcagttt attattaacg gcatcctgga
     6661 aatcattaaa acgtaccatt aaaaaaggcc cgaatgggcc tttcaactat tgcgtctgtt
     6721 cttcgagtgt cactggccag cggcgaaata taatgattga ccaaattaac gcggcaaaag
     6781 caggcaccgc gcccacataa ccaatcatcg acattgacca gtgcaaactc acctgattac
     6841 ctaccaacgc acccgccccg attccaatat taaatatccc ggagaatagc gccatcgcga
     6901 cgtcggtagc atctggtgcc agcgccagca ctttaacctg cataccaagc ccgatgatca
     6961 tcatcgcgat cccccagaaa atactcagca ccccgaggtg tatttcactg ttcgccgcag
     7021 gtaacagcaa tgccaggcac accagcaaca gcgcaatcgc cgtactcacc aacgcagacg
     7081 catactgatt acccagttta ccgaaaatca cgctgccaat aatgcccgca ccaccgagta
     7141 ataacagtaa tgccgtggca aagttggcgc tgaatcccgc aatgttttgt acaaaaggct
     7201 cgatatagct gtatgccgtg taatgggcgg tgacaaccac cacagttaac aaataaatgc
     7261 tcatcaatgc cgggcggcgg aacaatagcg ggaggctttt cagtgaaccg gaatgctcac
     7321 tgggcagtaa gggaagtaac ttaatcaggc acaaaagggt gataagcgcc ccaataccaa
     7381 tcgcgaagaa ggtcattcgc caaccgaaat actggcccac aatgcgcccg agaggtaaac
     7441 ctaagaccat cgccagtgct gtaccggtgg caattaaact caatgcctgt gctcgcttcc
     7501 cggccggagc catacggatc gccagagacg ccgtaatcga ccagaaaatc gcatgtgcaa
     7561 aagccacacc aatgcgactg atcaccagaa cggtaaagct ccacgacaaa aacgacagta
     7621 cgtggctggc aataaacacc acaaacaggc agatcagtaa tttgcgccgt tcaacctgac
     7681 tggtcattaa cataaaaggc aatgacatta gcgctactac ccatgcgtaa atggtcaaca
     7741 tgatgccgac ctgagcggtt tgcatgtgaa aactttgcgc aatgtcagag agcaggccaa
     7801 cagggacaaa ttcggtggtg ttgaagatga aggcggcgac tgccagcgta acgacccgta
     7861 gccacgccac tttgcgggaa acagtgtttg ttgtcataac agattatcgg gtgagttgcg
     7921 aaaaagtgat gaaatgatct taaaacggtt aatgatgtga atacaatgca tttgtgatct
     7981 ggatcgcgtt ttccgtttct ctgacgctga ttctttcacg cgtcagagag tcggtaacat
     8041 cacggtttaa tgacgttcag ggaggtctgg cgttttggag gttgcgggta taggtggtct
     8101 gacaggggag cgaggcctgt ttttcaccag gtcttcgtga gtaacttttg gcggcgtttc
     8161 attttttaca aacagtttga gggtcctgcg cgccatatgt ttgataattt tatcatcact
     8221 taatgatgct ggcatcaaca cgtcattcag atcactttca ttgggattca taaattggtg
     8281 atagtgcaat aacatattgg cgaaaaataa aaaaaagtcc tgaaaatgag gagattcttg
     8341 ttctgctaaa agggataaaa agcagatgca gacttcgggt agtacatgtt tgttagaacc
     8401 taatgaatcc atcaggcttt gcaataaatc ctcttttaat tcgccgtctg ttatgtcgag
     8461 aatggcggtg atcaagttag gatctttatt gacgaaccat ctgtgaacct cagagttact
     8521 ccataatcgg gggagtatga caaaattacc gcgagagata aaattcagga caagcaaatc
     8581 aaactggcgt aagtaatctg cagcggtatg gcgatccgga ggcaggggtt caatatgtgg
     8641 tgtatgcgct cttttgatgg gaacgccaag gttgatcatt ggaaattgtt caataaaatc
     8701 attcagaaac agaaatagca cacgctcgac gccggtctga aatacgcgcc ccatagtgac
     8761 aggctttggg ttgggagcgc aaaaatatta atttctccat catggcgaaa attatctaaa
     8821 taaatagggc ttaaatttat tactacctga aattcaatat tttctttgtg tcttccattc
     8881 ttgactggat aagtgagttt aaactgaacc ttttcattgt atagcgtgtc g
//
LOCUS       DAPHDX010000160         8853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPHDX010000160 DAPHDX010000000
VERSION     DAPHDX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.162"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..840
                     /gene="gadX"
                     /locus_tag="RH058_003903"
     CDS             16..840
                     /gene="gadX"
                     /locus_tag="RH058_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV3139419.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1208..1936
                     /gene="gadW"
                     /locus_tag="RH058_003904"
     CDS             1208..1936
                     /gene="gadW"
                     /locus_tag="RH058_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV3139420.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2299..5412)
                     /locus_tag="RH058_003905"
     CDS             complement(2299..5412)
                     /locus_tag="RH058_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV3139421.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5437..6594)
                     /gene="mdtE"
                     /locus_tag="RH058_003906"
     CDS             complement(5437..6594)
                     /gene="mdtE"
                     /locus_tag="RH058_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV3139422.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6654..6932
                     /locus_tag="RH058_003907"
     CDS             6654..6932
                     /locus_tag="RH058_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139423.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6933..7460)
                     /gene="gadE"
                     /locus_tag="RH058_003908"
     CDS             complement(6933..7460)
                     /gene="gadE"
                     /locus_tag="RH058_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV3139424.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8259..8831)
                     /gene="hdeD"
                     /locus_tag="RH058_003909"
     CDS             complement(8259..8831)
                     /gene="hdeD"
                     /locus_tag="RH058_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV3139425.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2377 a         2059 c         2065 g         2352 t
ORIGIN      
        1 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
       61 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      121 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      181 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      241 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      301 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      361 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      421 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      481 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      541 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      601 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
      661 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
      721 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
      781 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
      841 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
      901 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
      961 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1021 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1081 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1141 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1201 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1261 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1321 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1381 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1441 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1501 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1561 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1621 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     1681 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     1741 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     1801 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     1861 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     1921 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     1981 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2041 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2101 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2161 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2221 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2281 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2341 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2401 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2461 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2521 ttcgatgatt gcctctatcg gcgttttccc ttctttttgc atcatctcaa cggcaaattc
     2581 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     2641 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     2701 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     2761 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     2821 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     2881 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     2941 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3001 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3061 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3121 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3181 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3241 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3301 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3361 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3421 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3481 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3541 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3601 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     3661 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     3721 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     3781 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     3841 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     3901 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     3961 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4021 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4081 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4141 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4201 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4261 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4321 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4381 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4441 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4501 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4561 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4621 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     4681 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     4741 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     4801 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     4861 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     4921 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     4981 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5041 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5101 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5161 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5221 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5281 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5341 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5401 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5461 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5521 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5581 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     5641 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     5701 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     5761 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     5821 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     5881 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     5941 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6001 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6061 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6121 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6181 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6241 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6301 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6361 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6421 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6481 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6541 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6601 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     6661 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggtatagtg
     6721 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     6781 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     6841 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     6901 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     6961 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7021 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7081 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7141 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7201 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7261 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7321 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7381 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7441 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7501 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7561 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7621 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagttt gtaaacagta
     7681 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     7741 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     7801 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     7861 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     7921 tgcaatgtaa tcaatgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     7981 tagcatcaac gcttacggaa ctctttgttc actttcccac tgcactttta aaacattaaa
     8041 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8101 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8161 atagtgcgca attgatctac aacgctgcgt agcggagaga gtattaatcg gatcatagtc
     8221 acatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8281 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8341 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8401 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8461 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8521 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8581 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     8641 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     8701 acgaacggga aactgataca cagcaacccg acgataaaca acagcacggc aataaactgg
     8761 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     8821 atatataaca tagaaccacc ctataaaatt aag
//
LOCUS       DAPHDX010000161         8833 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPHDX010000161 DAPHDX010000000
VERSION     DAPHDX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.163"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            104..1339
                     /gene="moeA"
                     /locus_tag="RH058_003910"
     CDS             104..1339
                     /gene="moeA"
                     /locus_tag="RH058_003910"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV3139426.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVISKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            1339..2088
                     /gene="moeB"
                     /locus_tag="RH058_003911"
     CDS             1339..2088
                     /gene="moeB"
                     /locus_tag="RH058_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV3139427.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAAMIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2176..2838)
                     /gene="fsa"
                     /locus_tag="RH058_003912"
     CDS             complement(2176..2838)
                     /gene="fsa"
                     /locus_tag="RH058_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV3139428.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            2969..3868
                     /locus_tag="RH058_003913"
     CDS             2969..3868
                     /locus_tag="RH058_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV3139429.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3874..6306
                     /locus_tag="RH058_003914"
     CDS             3874..6306
                     /locus_tag="RH058_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV3139430.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHELCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6452..7267
                     /gene="ybiV"
                     /locus_tag="RH058_003915"
     CDS             6452..7267
                     /gene="ybiV"
                     /locus_tag="RH058_003915"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV3139431.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGNQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7419..8684
                     /locus_tag="RH058_003916"
     CDS             7419..8684
                     /locus_tag="RH058_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV3139432.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         2092 a         2356 c         2358 g         2027 t
ORIGIN      
        1 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctgtgg cgtttttctg
       61 acataatagg caaattcgat tttgcctccg aaggagtgtt ttcatggaat ttaccaccgg
      121 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgtcaccc cactgaccgc
      181 ccaggaaacg ctgccactgg tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
      241 gccgcttgat gttccggggt ttgataactc cgcaatggac ggctacgcgg tgcgtttagc
      301 cgatattgcc tccaggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta
      361 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
      421 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
      481 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
      541 tgcggttgtt ttcccggcgg gaactcgcct gactaccgct gaactgccag tgattgcttc
      601 actggggatt gccgaagttc cggtgattag taaagtgcgt gtagcgcttt tttctaccgg
      661 tgatgaactc cagttgcccg gtcagccact gggcgatggc caaatctacg ataccaaccg
      721 tctcgccgta cacctgatgt tagaacagtt gggatgcgag gtaatcaact tagggattat
      781 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
      841 ggtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa ccattcttga
      901 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccgggtaaac cgttcgcgtt
      961 cggtaaactt tctaatagct ggttctgcgg cctgccgggc aacccggttt cagcgacgct
     1021 gaccttctat caactggtac agcctttgct ggcaaaacta agcggtaaca ccgccagcgg
     1081 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggtcgcct
     1141 tgatttccag cgcggcgtgc tgcaacgcaa cgccgatggc gaactggaag tgacgaccac
     1201 cggacatcag ggttcacata tatttagctc ctttagcctc ggcaactgct ttatcgtgct
     1261 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac catttaacgc
     1321 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     1381 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     1441 ctggtcgtcg gcctcggtgg actcggctgc gcagcctcgc agtatctggc aagcgccggt
     1501 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     1561 acgctgcaca gtgatgccac ggtcgggcaa cctaaggttg aatccgcacg tgacgccctg
     1621 gcgcggatca acccgcatat tgcgattacg ccagtcaatg cactgctgga tgacgctgaa
     1681 cttgctgcga tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     1741 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtac cgctggtttc cggcgcggca
     1801 attcgtatgg aaggtcaaat cacggtcttc acttatcagg acggtgaacc atgctaccgc
     1861 tgcctcagcc gtttgtttgg tgaaaatgca ttaacctgcg tggaagcagg cgtaatggca
     1921 ccgttgattg gcgtgattgg ttcgttgcag gcaatggaag cgatcaagct gctggcaggt
     1981 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     2041 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     2101 ggcctgataa gacgcggcag gcatcggtgc actccgccac gtcatcagca atttatgagg
     2161 atgcagaacg tccggttaaa tcgacgttct gccaaacgct cccagccagt cctgttcaaa
     2221 cttcgccaca gcggcttcca ccgccggata gctaatcatc tgttgtgcca catccagtgg
     2281 cagcgtaatt gattcacatc ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa
     2341 actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt gtaagtcggt
     2401 cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag gcgcaacata
     2461 ttccgcacct gccagcgccg acagcagccc ttgtgctgcg ccatataccg cggttcccag
     2521 cgttggaatc ccttccgctt ttaacatctt aatagctgcc agcccctcgg cggtcaccgg
     2581 aactttcacc acgatatccg caataataga acgcaattta cgtgcgtcat tcaccatccc
     2641 ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg
     2701 aagttccgga agcacaacct ccagcggttt tttacccgcg gcgataatgc ttgggttagt
     2761 ggtcacaccc gccagcggaa aaatacgtga cagcgccttc accgcaacaa cgtctgaagt
     2821 atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat gtagactatc
     2881 acgggaaaac tctctgaaca gttgacgcac atcaatctaa ctttcattcg aaagtatttt
     2941 aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct actcgaccca
     3001 tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg
     3061 gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg
     3121 tctggaaggc tgcgagctgt gcgctaaggc agcgccggaa gtgattgagc gcgcgctgaa
     3181 tggtttgctt attcatcggg aaaagttaac cccggagcat tttacggcgc taactgactg
     3241 ctgcccgaca caggcgttaa ccgtgtgtgg ggaagtgaaa agcgttgagg agatcatgac
     3301 gaccgttctg cgcgataaac cgttttacga tcgcagcggc ggcggcttaa cgctttctgg
     3361 cggtgaaccg tttatgcagc cggaaatggc gacggcgctg ctgcaagcca gccacgaggc
     3421 cggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata tcgccccttc
     3481 tctgccctat atcgatctgt ttcttgccga tttaaaacac gttgccgacg cgccgtttaa
     3541 acagtggacc gacggtaacg ccgccagagt gctggataac ctgaaaaaac tcgctgcagc
     3601 gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg acgaaacctc
     3661 tgtgaaagcc attaccgatt ttgccgccga cgagctgcac gttggtgaaa ttcatttcct
     3721 gccctaccac acgctgggca tcaacaaata tcacttactt aatctaccct atgacgcccc
     3781 ggaaaaaccg cttgatgcgc cagaactgct cgactttgcc cagcagtatg cctgccagaa
     3841 agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact ggacacgctc
     3901 agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt
     3961 accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa gccgatcccg
     4021 gtacgtcgcg cgctggcgct ggcacatcac ctggcgaatc gcactatctg gatcaagcac
     4081 gatgagttga tcatcggcaa ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa
     4141 tatactgtct cctggatcga aaaagagatt gatgatctgg cggatcgtcc cggtgctggt
     4201 tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaat tgtgcccgtg gtggcgcggt
     4261 cagaccgtac aggatcgctg ctacggcatg tttaccgatg agcaaaaagg tctgctggcg
     4321 accggcatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct ggcggtgaat
     4381 ttcccgctgc tgctggaaaa aggtcttgat ggtctgcgcg agaaagtagc ggaacgtcgc
     4441 tcgcgcatca acctgacggt gctggaagat ttacacggtg agcaattcct gaaagcgatt
     4501 gatatcgtgc tggtggcagt cagtgaacac attgaacgtt tcgctgccct ggcgcgtgaa
     4561 atggccgcga ccgaaacccg cgaaagccgt cgcgatgaac tgctgacgat agcagaaaac
     4621 tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca actgtgttac
     4681 ttcatccagt tgattttgca gatcgaatct aacggtcact cggtatcgtt tggtcgtatg
     4741 gaccagtatc tctacccata ctatcgccgc gacgttgaac tcaaccagac actggatcgc
     4801 gaacacgcca tcgaattgct gcatagctgc tggctgaaac tgctggaagt gaacaagatc
     4861 cgctccggct cgcactcgaa agcctctgcg ggaagtccgc tgtatcagaa cgtcaccatt
     4921 ggcgggcaaa atctggttga tggtcaacca atggacgcgg tgaatcccct ctcttacgcg
     4981 atccttgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt gcgttaccat
     5041 gcaggaatga gtaacgattt cctcgacgcc tgcgtacagg tgatccgctg cggcttcggg
     5101 atgccggcgt tcaacaacga cgaaatcgtg atcccggaat ttattaaact cggtattgaa
     5161 ccgcaggacg cttacgacta cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa
     5221 tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat gctggcggcg
     5281 ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc tgccacaaga aaaagcgttg
     5341 tcggcaggta actttaacaa tttcgatgaa gtaatggacg cgtgggatac gcaaatccgt
     5401 tactacaccc gcaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac
     5461 gtgcacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc
     5521 aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggttggcat tgccaacctc
     5581 ggcaacagcc tggcggcagt gaagaaactg gtatttgaac agggcgcgat tggtcagcaa
     5641 cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca actgcgtcag
     5701 cggctgatta acggtgcgcc gaagtacggc aacgacgatg ataccgtcga tacgctgctg
     5761 gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataaccc acgctacggt
     5821 cgtggtccgg ttggcggcaa ctattacgcg ggtacgtcgt ctatctccgc taacgtaccg
     5881 tttggcgcgc agacgatggc aacgccggac ggacgtaaag cgcatacccc gctggcagaa
     5941 ggcgcaagcc cggcttccgg tactgatcat cttggcccta ctgcggtcat tggctcggtg
     6001 ggtaaactgc ctacggcagc gattctcggc ggggtgttgc tcaaccagaa actgaatcca
     6061 gcaacactgg agaacgaatc tgacaagcag aaactgatga tcctgctgcg caccttcttt
     6121 gaagtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac gctgctggaa
     6181 gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg ctattccgcc
     6241 ttcttcaccg cgctctctcc agacgcacag gacgatatca tcgcccgtac tgaacatatg
     6301 ctgtaatctt ctcagcccgg cggcgtcgtc gccgggctaa atcattcaca tcatcaattt
     6361 catccttact ttcattcgaa atataatttg tgctctgcgt cacattatta ttacactgtt
     6421 acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg tcacagacat
     6481 ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacgtttta tggcgcaata
     6541 tcaggaactg aaaaagcgcg gcattaagtt cgttgtcgcc agcggtaatc agtattacca
     6601 gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg aaaacggcgc
     6661 actggtttac gaacatggca atcaactgtt ccacggcgaa ctgacccgac atgaatcgcg
     6721 gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct gcggtctgca
     6781 aagtgcatat gtcagcgaaa acgcccccga agcattcgtc gcactgatgg caaaacacta
     6841 ccatcgcctg aaacctgtaa aagattatca ggagattgac gacgtactgt tcaaattttc
     6901 gctcaacctg ccggatgaac aaatcccgtt agtgatcgac aaactgcacg tagcgctgga
     6961 tggcattatg aaacccgtta ccagtggttt tggctttatc gacctgatta ttcccggtct
     7021 acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt
     7081 ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc gttattcctt
     7141 tgcgatgggc aatgctgcgg aaaacattaa acaaatcgct cgttacgcta ccgatgataa
     7201 taatcatgaa ggcgcgctta atgtgattca ggcggtgctg gataacacat ccccttttaa
     7261 cagctgacct ctctaccgga gccatgcgct ccggttttcc cttcttttta catcctgtac
     7321 atcacatcaa atcattaaac ttgcattaac ttatttataa cttatattgt cacatgcaaa
     7381 tcatcatagt ttcgaatgaa agttaatgcg aggtggttat ggcttctact tttaccagcg
     7441 acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc
     7501 agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc acgcgagtgg
     7561 ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg tcttatgccg
     7621 atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt cgcggttgtg
     7681 cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg
     7741 actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat aatttcttcg
     7801 ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag gcgcaaatgc
     7861 aggctcgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat
     7921 ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac cctgaccgta
     7981 tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc gactccgggg
     8041 cactggaacg ctggctgctt ccagcgtatc agcgcgtttt cgccaacgtc tttaatggca
     8101 atctggcgca atatgatccc tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg
     8161 tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggaca gcgctctctg
     8221 atatgctgcc tggtcagggg ctgctgcacg tcgtgcccat tcctgaagcc atggcgtacg
     8281 tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc gtagcgcccg
     8341 gaagagtatt gccggtatca gagcaatggc atccactgtt gattgaggcg ttaaccagca
     8401 ttccaaaact cgaagccgga gactccgtct ggtggcactg cgacgtcatc cattccgttg
     8461 cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg gcaccgatgt
     8521 gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc
     8581 cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt acgcttgccg
     8641 acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta atggaagtga
     8701 attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca agtgcgcttt
     8761 cttaatgcct gatgcggcgt taacgcctta tctggcctac aaagtcctgc agattcaata
     8821 tattacaggt act
//
LOCUS       DAPHDX010000162         8667 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPHDX010000162 DAPHDX010000000
VERSION     DAPHDX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.164"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(298..561)
                     /gene="rpsT"
                     /locus_tag="RH058_003917"
     CDS             complement(298..561)
                     /gene="rpsT"
                     /locus_tag="RH058_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV3139433.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            664..882
                     /locus_tag="RH058_003918"
     CDS             664..882
                     /locus_tag="RH058_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV3139434.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            890..1831
                     /gene="ribF"
                     /locus_tag="RH058_003919"
     CDS             890..1831
                     /gene="ribF"
                     /locus_tag="RH058_003919"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV3139435.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1874..4690
                     /gene="ileS"
                     /locus_tag="RH058_003920"
     CDS             1874..4690
                     /gene="ileS"
                     /locus_tag="RH058_003920"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV3139436.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4690..5184
                     /gene="lspA"
                     /locus_tag="RH058_003921"
     CDS             4690..5184
                     /gene="lspA"
                     /locus_tag="RH058_003921"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV3139437.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5272..5721
                     /gene="fkpB"
                     /locus_tag="RH058_003922"
     CDS             5272..5721
                     /gene="fkpB"
                     /locus_tag="RH058_003922"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3139438.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5723..6673
                     /gene="ispH"
                     /locus_tag="RH058_003923"
                     /gene_synonym="lytB"
     CDS             5723..6673
                     /gene="ispH"
                     /locus_tag="RH058_003923"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV3139439.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6739..7653
                     /gene="rihC"
                     /locus_tag="RH058_003924"
     CDS             6739..7653
                     /gene="rihC"
                     /locus_tag="RH058_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV3139440.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNFAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7820..8641
                     /gene="dapB"
                     /locus_tag="RH058_003925"
     CDS             7820..8641
                     /gene="dapB"
                     /locus_tag="RH058_003925"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV3139441.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1979 a         2157 c         2466 g         2065 t
ORIGIN      
        1 ttaattcaaa aatagagtgg tcttaagttt aatacctcaa tgtgtatcac aatatccaca
       61 ttctttgtag gggagtctgg agattgagta gatattcttg ttcagaatgt atcagccgat
      121 ggttctacga ttcttaagcc acgaagagtt cagaaagtgt aacggcatgt ctgcaaaagc
      181 cagcatcttt tgactatctg gcaaccggta gtgtgttctc tcacgcatca taaaagcagc
      241 aggcataaaa aaacccgctt gcgcgggctt tttcacaaag cttcagcaaa ttggcgatta
      301 agccagtttg ttgatctgtg cagtcaggtt agccttatga cgtgcagctt tgtttttgtg
      361 gatcagacct ttagcagcct gacggtccac gatcggttgc atttcgttaa atgctttctg
      421 tgcagcagct ttgtcgccag cttcgatagc tgcgtatact ttcttgatga aagtacgcat
      481 catagagcga cggcttgcgt tgtgcttacg agccttttca gactgaatgg cgcgcttctt
      541 agctgatttg atattagcca aggtccaact cccaaatgtg ttctatatgg acaattcaaa
      601 ggccgaggaa tatgcccttt tagccttctt ttgtcaatgg atttgtgcaa ataagcgccg
      661 ttaatgtgcc ggcactcgtt acgtagtgat ggcgcaggat tctaccagct tgcggggtgt
      721 gaatacagct tttccgcgat aaaaattgca gcaggcggtc actttcttcc cgtgatttgc
      781 gccatggcaa tgaaaagcca cttctttctg atttcggtac tcaatcgccg gttaaccttg
      841 accgctgtac aaggtatact cggacgattt tcactgtttt gagccagaca tgaagctgat
      901 acgcggcata cataatctca gccaggcccc gcaagaaggg tgtgtgctga ctattggtaa
      961 tttcgacggc gtgcatcgcg gtcatcgcgc gctgttacag ggcttgcagg aagaagggcg
     1021 caagcgcaac ttaccggtga tggtgatgct ttttgaacct caaccactgg aactgtttgc
     1081 taccgataaa gccccggcac ggctgacccg gctgcgggaa aaactgcgtt accttgcaga
     1141 gtgtggcgtt gattacgtgc tgtgcgtgcg tttcgacagg cgtttcgcgg cgttaaccgc
     1201 gcaaaatttc atcagcgatc ttctggtgaa gcatttgcgc gtaaaatttc ttgccgtagg
     1261 tgatgatttc cgctttggcg ctggtcgtga aggcgatttc ttgttattac agaaagctgg
     1321 catggaatac ggcttcgata tcaccagtac gcaaactttt tgcgaaggtg gcgtgcgtat
     1381 cagcagcacc gctgtgcgtc aggcgctggc ggatgacaat ctggctctgg cagaaagttt
     1441 actggggcac ccgtttgcca tctccgggcg tgtagtccac ggtgatgaat tagggcgcac
     1501 tataggtttc ccgacggcga atgtaccgct acgccgtcag gtttccccgg tgaaaggggt
     1561 ttatgcggta gaagtgctgg gcctcggtga aaagccgtta cccggcgtgg caaacattgg
     1621 aacacgccca acggttgccg gtattcgcca gcagctggaa gtgcatttgt tagatgttgc
     1681 aatggacctt tacggtcgcc atatacaagt agtgctgcgt aaaaaaatac gcaatgagca
     1741 gcgatttgcg tcgctggacg aactgaaagc gcagattgcg cgtgatgaat taaccgcccg
     1801 cgaatttttt gggctaacaa aaccggctta agcctgttat gtaatcaaac cgaaacacgg
     1861 aaccgagaat ctgatgagtg actataaatc aaccctgaat ttgccggaaa cagggttccc
     1921 gatgcgtggc gatctcgcca agcgcgaacc gggaatgctg gcgcgttgga ctgatgatga
     1981 tctgtacggc atcatccgtg cggctaaaaa aggcaaaaaa accttcattc tgcatgatgg
     2041 ccctccttat gcgaatggca gcattcatat tggtcactcg gttaacaaga ttctgaaaga
     2101 cattatcgtg aagtccaaag ggctttccgg ttatgactcg ccgtatgtgc ctggctggga
     2161 ctgccacggt ctgccgatcg agctgaaagt agagcaagaa tacggtaagc cgggtgagaa
     2221 attcaccgcc gccgagttcc gcgccaagtg ccgcgaatac gcggcgaccc aggttgacgg
     2281 tcaacgcaaa gactttatcc gtctgggcgt gctgggcgac tggtcgcacc cgtacctgac
     2341 catggacttc aaaactgaag ccaacatcat ccgcgcgctg ggcaaaatca tcggcaacgg
     2401 tcacctgcac aaaggcgcga agccagttca ctggtgcgtt gactgccgtt ctgcgctggc
     2461 ggaagcggaa gttgagtatt acgacaaaac ttctccgtcc atcgacgttg cttttcaggc
     2521 ggtcgatcag gatgcactga aagcaaaatt tgccgtaagc aacgttaacg gcccaatctc
     2581 gctggtgatc tggaccacta cgccgtggac tctgcctgcg aaccgcgcaa tctctattgc
     2641 acctgatttc gactatgcgc tggtgcagat cgacggtcag gccgtgattc tggcgaaaga
     2701 tctggttgaa agcgtaatgc agcgtatcgg cgtgaccgat tacaccattc tcggcacggt
     2761 aaaaggtgcg gagcttgagt tgctgcgctt tacccatccg tttatgggct tcgacgttcc
     2821 ggcaatcctc ggcgatcacg ttaccctgga tgccggtacc ggtgccgttc acaccgcgcc
     2881 tggccacggc ccggacgact atgtgatcgg tcagaaatac ggcctggaaa ccgctaaccc
     2941 ggttggcccg gacggcactt atctgccggg cacttatccg acgctggatg gcgtgaacgt
     3001 cttcaaagcg aacgacatcg tcgttgcgct gctgcaggaa aaaggcgcgc tgctgcacgt
     3061 tgagaaaatg cagcacagct atccgtgctg ctggcgtcac aaaacgccga tcatcttccg
     3121 cgcgacgccg cagtggttcg tcagcatgga tcagaaaggt ctgcgtgcgc agtcactgaa
     3181 agagatcaaa ggcgtgcagt ggatcccgga ctggggccag gcgcgtatcg agtcgatggt
     3241 tgctaaccgt cctgactggt gtatctcccg tcagcgcacc tggggcgtac cgatgtcact
     3301 gttcgtgcac aaagacacgg aagagctgca tccgcgtact ctcgaactaa tggaagaagt
     3361 ggcaaaacgc gttgaagttg acggcatcca ggcgtggtgg gatcttgatg cgaaagagat
     3421 cctcggcgat gaagctgatc agtacgtgaa agtgccggac acattggatg tatggtttga
     3481 ctccggatct acccactctt ctgttgttga cgtgcgtccg gaatttgccg gtcacgcagc
     3541 ggacatgtat ctggaaggtt ctgaccagca ccgtggttgg ttcatgtctt ccctgatgat
     3601 ctccaccgcg atgaaaggca aagcgccgta tcgtcaggta ctgacccacg gctttaccgt
     3661 ggatggtcag ggtcgcaaga tgtctaaatc catcggcaat accgtttcgc cgcaggatgt
     3721 gatgaacaaa ctgggcgcgg atattctgcg tctgtgggtg gcatcaaccg actacaccgg
     3781 tgaaatggcc gtttctgacg agatcctgaa acgtgctgcc gatagctatc gtcgtatccg
     3841 taacaccgcg cgcttcctgc tggcaaacct gaacggtttt gatccagcaa aagatatggt
     3901 gaaaccggaa gagatggtgg tactggatcg ctgggccgta ggttgtgcga aagcggcaca
     3961 ggaagacatc ctcaaggcgt acgaagcata cgatttccac gaagtggtac agcgtctgat
     4021 gcgcttctgc tccgttgaga tgggttcctt ctacctcgac atcatcaaag accgtcagta
     4081 caccgccaaa gcggacagtg tggcgcgtcg tagctgccag actgcgctgt atcacatcgc
     4141 agaagcgctg gtgcgctgga tggcaccaat cctctccttc accgctgatg aagtgtgggg
     4201 ctacctgccg ggcgaacgtg aaaaatacgt cttcaccggt gagtggtacg aaggcctgtt
     4261 tggcctggca gacagtgaag cgatgaacga tgcgttctgg gacgagctgt tgaaagtgcg
     4321 tggcgaagtg aacaaagtca ttgagcaagc gcgtgccgac aagaaagtgg gtggctcgct
     4381 ggaagcggca gtaaccttgt atgcagaacc ggaactgtcg gcgaaactga ccgcgctggg
     4441 cgatgaatta cgatttgtcc tgttgacctc cggcgctacc gttgcagact ataacgacgc
     4501 acctgctgat gctcagcaga gcgaagtact caaagggctg aaagtcgcgt tgagtaaagc
     4561 cgaaggtgag aagtgcccac gctgctggca ctacacccag gatgtcggca aggtggcgga
     4621 acacgcagaa atctgcggcc gctgtgtcag caacgtcgcc ggtgacggtg aaaaacgtaa
     4681 gtttgcctga tgagtcaatc gatctgttca acagggctac gctggctgtg gctggtggta
     4741 gtcgtgctga ttatcgatct gggcagcaaa tacctgatcc tccagaactt tgctctgggg
     4801 gatacggtcc cgctgttccc gtcgcttaat ctgcattatg cgcgtaacta tggcgcggcg
     4861 tttagtttcc ttgccgatag cggcggctgg cagcgttggt tctttgccgg tattgcgatt
     4921 ggtattagcg tgatcctggc agtgatgatg tatcgctcga aggccacgca gaagctaaac
     4981 aatatcgctt atgcgctgat tattggcggc gcgctgggca acctgttcga ccgcctgtgg
     5041 cacggcttcg ttgtcgatat gatcgacttc tacgtcggcg actggcactt cgccaccttc
     5101 aaccttgccg atactgccat ttgtgtcggt gcggcactga ttgtgctgga aggttttttg
     5161 ccttctaaag cgaaaaaaca ataataaacc ctgccggata aggcctacag attgctgcga
     5221 aatcgtaggc cgcatccggc aaaaatcctc aaaatataag agcaaacctg catgtctgaa
     5281 tctgtacaga gcaatagcgc cgtcctggtg cacttcacgc taaaactcga cgatggcacc
     5341 accgccgagt ctacccgcaa caacggtaaa ccggcgctgt tccgcctggg tgatgcttct
     5401 ctttctgaag ggctggagca acacctgttg gggctgaaag tgggcgataa aaccaccttc
     5461 tcgttggagc cagatgcggc gtttggcgtg ccgtcaccgg acctgattca gtacttctcc
     5521 cgccgtgaat ttatggatgc aggcgagcca gaaattggcg caatcatgct ttttaccgca
     5581 atggatggca gtgagatgcc tggcgtgatc cgcgaaatta acggcgactc cattaccgtt
     5641 gatttcaacc atccgctggc cgggcagacc gttcattttg atattgaagt gctggaaatc
     5701 gatccggcac tggaggcgta acatgcagat cctgttggcc aacccgcgtg gtttttgtgc
     5761 cggggtagac cgcgctatca gcattgttga aaacgcgctg gccatttacg gcgcaccgat
     5821 atatgtccgt cacgaagtgg tacataaccg ctatgtggtc gatagcttgc gtgagcgtgg
     5881 ggctatcttt attgagcaga ttagcgaagt accggacggc gcgatcctga ttttctccgc
     5941 acacggtgtt tctcaggcgg tacgtaacga agcaaaaagt cgcgatttga cggtatttga
     6001 cgcgacctgt ccgctggtga ccaaagtgca tatggaagtc gcccgcgcta gtcgccgtgg
     6061 cgaagaatct attctcatcg gtcacgccgg gcacccggaa gtggaaggca cgatgggtca
     6121 gtacagcaac ccggaagggg gaatgtatct ggttgaatca ccagacgatg tgtggaaact
     6181 gacggtcaaa aacgaagaga aactctcctt tatgacccag accacgctgt cggtggatga
     6241 cacgtctgat gtgatcgacg cgctgcgtaa acgcttcccg aaaattgtcg gtccgcgcaa
     6301 agatgacatc tgctacgcca cgactaaccg acaggaagcg gtacgtgccc tggccgaaca
     6361 ggcggaagtt gtgctggtgg tcggttcgaa aaactcctct aactccaacc gtctggcgga
     6421 gctggcccag cgtatgggca aaagcgcgtt tttgattgat gatgcgaaag atatccagga
     6481 agagtgggtg aaagaggtta aatgcgtcgg cgtgactgcg ggcgcatcgg ctccggatat
     6541 tctggtgcag aatgtggtgg cacgtttgca acagctgggc ggtggtgaag ccattccgct
     6601 ggaaggacgt gaagaaaaca ttgttttcga agtgccgaaa gagctgcgtg tcgatattcg
     6661 tgaagtcgat taagtcatta gcagcctaag ttatgcgaaa atgccggtct tgttaccggc
     6721 attttttatg gagaaaacat gcgtttacct atcttcctcg atactgaccc cggcattgac
     6781 gatgccgtcg ccattgccgc cgcgattttt gcacccgaac tcgacctgca actgatgacc
     6841 accgtcgcgg gtaatgtctc ggttgagaaa actacccgca atgccctgca actgctgcat
     6901 ttctggaatg cggagattcc gctcgcccaa ggggccgctg tgccgctggt acgcgcaccg
     6961 cgtgatgcgg catctgtgca cggcgaatcg ggaatggctg gctacgactt tgttgagcac
     7021 aaccgaaagc cgctcgggat accggcgttt ctggcgattc gggatgccct gatgcgtgca
     7081 ccagagcctg ttaccctggt ggccattggc ccgttaacca atattgcgct gttactttca
     7141 caatgcccgg aatgcaagcc gtatattcgc cgtctggtga tcatgggtgg ttctgccgga
     7201 cgcggcaact gtacgccaaa cgccgagttt aatttcgctg ccgatccaga agcggctgcc
     7261 tgtgtcttcc gtagcggaat cgaaatcgtc atgtgcggtc tggatgtcac caatcaggca
     7321 atattaaccc ctgactatct cgctactttg ccggagttga atcgcaccgg aaaaatgctt
     7381 catgcactgt ttagccacta ccgtagcggc agtatgcaaa gcggtctgcg aatgcacgat
     7441 ctctgcgcca tcgcctggct ggtgcgcccg gacttattca ccctcaagcc gtgttttgtc
     7501 gcggtagaaa ctcaaggcga atttacctca ggcacgacgg tggttgatat cgacggttgc
     7561 ctgggcaaac cagccaatgt aaaggtggca ttggatctgg atgtgaaagg cttccagcag
     7621 tgggtggctg aggtgctggc tctggcgtcg taacctgtca catgttatcg gcatgcagtc
     7681 attcatcgac tcatgccttt cactgatatc cccccctgtt tatcattaat ttctaattat
     7741 cagcgttttt ggctggcggc gtagcgatgc gctggttact ctgaaaacgg tctatgcaaa
     7801 ttaacaaaag agaatagcta tgcatgatgc aaacatccgc gttgccatcg cgggagccgg
     7861 ggggcgtatg ggccgccagt tgattcaggc ggcgctggca ttagaaggcg tgcagctggg
     7921 cgcggcgctg gagcgcgaag gatcttcttt actgggcagc gacgccggtg agctggctgg
     7981 tgcagggaaa acaggcgtta ccgtgcaaag cagcctcgat gcggtaaaag atgattttga
     8041 tgtgtttatc gattttaccc gtccggaagg tacgctgaac catctcgctt tttgtcgcca
     8101 gcatggcaaa gggatggtga tcggcactac ggggtttgac gaagccggta aacaggcgat
     8161 tcgtgacgcc gccgccgata ttgcgattgt ctttgccgcc aattttagcg ttggcgttaa
     8221 cgtcatgctt aagctgctgg agaaagcagc caaagtgatg ggtgactaca ccgatatcga
     8281 aattattgaa gcacatcata gacataaagt tgatgcgccg tcaggcaccg cactggcaat
     8341 gggagaggcg atcgcccacg cccttgataa agatctgaaa gattgcgcgg tctacagtcg
     8401 tgaaggccac accggtgaac gtgtgcctgg caccattggt tttgccaccg tgcgtgcagg
     8461 tgacatcgtt ggcgaacata ccgcgatgtt tgccgatatt ggcgagcgtc tggagatcac
     8521 ccataaggcg tccagccgca tgacatttgc taacggcgcg gtaagatcgg ctttgtggtt
     8581 gagtggtaag gaaagtggtc tttttgatat gcgagatgtg cttgatctca atagtttgta
     8641 accacaaaat atttgttatg gtgcaaa
//
LOCUS       DAPHDX010000163         8625 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPHDX010000163 DAPHDX010000000
VERSION     DAPHDX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.165"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            310..822
                     /locus_tag="RH058_003926"
                     /pseudo
     CDS             310..822
                     /locus_tag="RH058_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
     gene            885..1589
                     /locus_tag="RH058_003927"
     CDS             885..1589
                     /locus_tag="RH058_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV3139442.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(1642..1749)
                     /locus_tag="RH058_003928"
                     /pseudo
     CDS             complement(1642..1749)
                     /locus_tag="RH058_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1875..4760
                     /locus_tag="RH058_003929"
     CDS             1875..4760
                     /locus_tag="RH058_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV3139443.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            4830..5705
                     /locus_tag="RH058_003930"
     CDS             4830..5705
                     /locus_tag="RH058_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV3139444.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(5859..6674)
                     /locus_tag="RH058_003931"
     CDS             complement(5859..6674)
                     /locus_tag="RH058_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV3139445.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(7010..7846)
                     /locus_tag="RH058_003932"
     CDS             complement(7010..7846)
                     /locus_tag="RH058_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDV3139446.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7846..>8625)
                     /locus_tag="RH058_003933"
                     /note="aph(3'')-Ib"
     CDS             complement(7846..>8625)
                     /locus_tag="RH058_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV3139447.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1983 a         2436 c         2320 g         1886 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcggagg
       61 tggctcaacc tgcgaaaaga aacgagttgc tacgtaagtc cgagaacatg ctttccatgg
      121 tcttgcaccg gttgtgttcc ggtctatctc tcattttaag cggctttttt ctcaaatgcc
      181 accggcgatt tccagccgag tgctgatgaa tcccctaatg atttttatca aaatcattaa
      241 gttaaggtag atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca
      301 gacaacaaaa tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa
      361 ttacacttaa aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa
      421 actctcactc ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac
      481 atcaaacgaa tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta
      541 taccgttggc atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac
      601 tggtctgata tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac
      661 ggtcgttctg ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa
      721 gctcatgacc aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc
      781 attgtcagtg atgctggctt taaagtgcca tggtataaat cggcactgtt gcaaatagtc
      841 ggtggtgata aacttatcat ccccttttgc tgatggagct gcacatgaac ccattcaaag
      901 gccggcattt tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca
      961 tcagttaccg tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca
     1021 cgatttaccg ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg cgctggtact
     1081 ggcgtaaccc ttccgatctt tgcccgtggc acatggatga aacctacgtg aaggtcaatg
     1141 gccgctgggc gtatctgtac cgggccgtcg acagccgggg ccgcactgtc gatttttatc
     1201 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
     1261 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
     1321 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
     1381 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
     1441 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
     1501 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
     1561 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
     1621 cgctaacttt gcaacagtgc catcatagat attgtcccgt tcgacacctg cggcgcgcaa
     1681 ggcgtcgtgc tgcaggtcga gagactgcga gccatcggct ttggagacgc gggcatatcc
     1741 gatcagcatg tatcacaaac gttggtttga ggcggcgctt cggccacgat tgcattgacc
     1801 tctggaagtg tatctcaacc agcttcataa acaaagcgtc ttgaacgcta tcagattttg
     1861 aaaaaggaac atgtatgccg cgtcgcgtca ctctaaccga tcggcagaaa gacgcgctgt
     1921 tgcgcttgcc gacttcacag acggatttgc tcaagcacta tacgctgagt gatgaagacc
     1981 ttgggcatat caggctgcgt cggcgcgctc acaacaggtt cggcttcgcc ctgcaattgt
     2041 gtgtcctgcg ctatcccggc cgggtgctgg ctccaggcga actgatccct gcagaggtca
     2101 tcgaatttat cggagcgcag cttggcctgg gtgccgacga tctcgtagac tatgctgccc
     2161 gcgaggaaac acggcacgag catcttgccg agttacgggg gctctacggc ttccgcacct
     2221 tctccggacg tggtgcgagc gagctgaagg aatggttgtt ccgagaagcc gagatggcgg
     2281 tgtcgaacga ggatatcgcc cgtcgcttcg tagccgagtg ccgacgcacc cgcactgtcc
     2341 ttcccgcgac atccacgatc gagcggcttt gtgccgcggc tctcgtcgat gccgagcgac
     2401 gcatcgagac gaggatcgcc agtcggctgc ctatgtcgat ccgagaacag ttgctggcat
     2461 tgctcgagga gacggctgat gatcgggtga cccgttttgt gtggctgcgc cagttcgagc
     2521 ctggctcgaa ctcttcgtcg gccaaccggc tgctcgaccg gctcgaatat ctgcaacgca
     2581 tcgatctccc cgaggatctg cttgccggcg ttcctgccca tcgggtgact cgtctgcgca
     2641 ggcagggtga acggtattat gccgacggca tgcgcgatct cccggaggac aggcggcttg
     2701 cgatcttggc tgtttgcgtc tcggaatggc aggcgatgtt ggccgacgca gtggtcgaaa
     2761 cccacgaccg gatcgtcggc cgtctctacc gtgcttcgga gcgtatttgc catgcaaagg
     2821 tcgcagacga agcgggggtg gtgcgtgaca ccctgaaatc cttcgccgag atcgggggcg
     2881 ccctggtcga tgcacaggat gatggccagc cgctgggcga tgtcatcgcg agtgggtcag
     2941 ggtgggacgg cttaaaaacc cttgttgcaa tggcaaccag gctgaccgcc accatggccg
     3001 acgatccgct caatcatgtg ctcgacggtt atcaccgctt ccgccgatac gctccacgca
     3061 tgttgcgcct gctcgatctg cgagctgcgc ccgttgcact gccgcttctg gaagcggtga
     3121 cggcccttcg taccggtttg aacgatgccg cgatgaccag cttcttgcgg cccagctcga
     3181 aatggcatcg ccaccttcgg gcccagaggg ctggcgacgc tcgcctatgg gagatcgcgg
     3241 tgctgttcca tctgcgcgat gcgttccgct ccggagatgt ctggcttact aggtcccggc
     3301 gctatggcga tctgaaacac gcactcgttc cggcacaatc catcgcggaa ggcggtcgtc
     3361 tcgctgtgcc attgcggccg gaggaatggc tggcagaccg gcaagctcgc ctcgacatgc
     3421 ggttgcgcga gcttggccgt gccgctcgcg caggcacgat cccgggcggg tcgattgaaa
     3481 acggcgttct gcatatcgag aaactcgaag ccgccgcgcc gacaggcgcc gaagatctgg
     3541 tgctcgatct ctacaagcag atcccgccca cgcgcatcac cgatctcctg ctggaggtgg
     3601 atgcggcgac cggcttcacc gaagcgttca cccatctgcg cacaggagca ccctgcgctg
     3661 accggatcgg gctaatgaac gttatcttgg cggaagggat caacctcggc ttgcgcaaaa
     3721 tggcggatgc gacaaacacc cacaccttct gggaattgat ccgcattgga cggtggcatg
     3781 tcgagggcga agcctatgac cgggcgctgg ccatggtggt cgaggcacag gcagcgttac
     3841 ccatggcccg gttctggggc atgggcacgt cggcttcgag cgacggacag ttcttcgtcg
     3901 ctacagagca aggtgaggcc atgaacctgg tcaacgcgaa atatggcaat accccgggcc
     3961 tgaaagccta tagccacgtc tccgaccaat atgcgccgtt cgcaacccag gtgattcctg
     4021 caacggcaag cgaagcgcct tacatcctcg atggcctgct gatgaacgat gctggacgcc
     4081 atatccgcga gcagttcacc gacacgggcg gcttcaccga tcacgtcttt gccgcatgtg
     4141 ccattctcgg ctaccggttc gctccgcgca tccgcgacct gccatccaaa cggctctacg
     4201 cgttcaatcc gtcggccgcc ccggcgcacc tgcgagcgtt gatcggcgga aaggtcaacc
     4261 aagccatgat cgagcgcaat tggcccgaca tcctgcgcat cgccgccacc attgctgccg
     4321 ggaccgtcgc gccaagccag attctgcgga aactcgcctc ctatccgcgg cagaacgagc
     4381 tcgcgacagc cctgcgggaa gtcggtcgcg tcgagcgcac cctgttcatg atcgactgga
     4441 ttctggatgc cgaactccaa cggcgtgccc agatcgggct caacaaaggc gaagctcatc
     4501 atgcgctgaa gcgggcaatc agcttccacc gccgcggtga aatccgcgac cgttccgccg
     4561 aaggccagca ttaccgcatc gccggcatga atctgctcgc cgccatcatc atcttctgga
     4621 acaccatgaa gctcggcgag gtcgttgcaa accagaaacg cgatggaaag ctgctatcgc
     4681 ccgatctctt ggcccatgtt tcgccgctcg gatgggaaca catcaatctc accggagaat
     4741 atcgctggcc aaagccttag cgtaggattc cgccccctcc cgcaaacgac cccgtgatga
     4801 ccctgcaggc atcccctgag acccggttaa tgcgcgccga ggtgcagcgg cattatccgc
     4861 acatctggca ggagctggag gggctggcgc agggactgga ggccactgtt gatcaggtct
     4921 ttgcctggaa ctgccgtggc gatctggtgc gctcaacgtc ggatggctgt acgacggtgg
     4981 ccggggtaaa cgccgcaggc gagcggatta tcgcgcataa tgaagacggt tttcctcagt
     5041 tacgggatga atgcttgctg gctaccatta ctccgagtcg cggtctgagc tttacgagtt
     5101 ttgcctatcc ggggtcgatt tgcggccaca cctttgcggt aaatgaaaaa ggtatcgtta
     5161 acacggtgaa taacatccgt gcagtgtatc gtccgcaggg attaccgcgt cagatcctgg
     5221 ctcgcgcctc gctgaatgcg aacaccctcg acgaggccat cactatactt accggccagc
     5281 cgcgatccgg ggcgtttcac catacgctcg ggcagctggg cgatgcgcgg ctgttcagcg
     5341 ttgaggccac cggtcaaggc tgttccgttc tacccctgac gacggtaacc gggcatgcca
     5401 accatctggt gcatgcggca ctggctggcg ttgagcagat cgttaccgac agttcagcgt
     5461 cgcggcagtt acgcctcgtg cagtggcggg aaacgcaacc tccgtttgac gccgcggcgg
     5521 caaaaaccat tctctcagat acccatgacg cagaactgcc aatttaccgg ctggctgccg
     5581 acgatccgga tgaggagaac accctcgcca cggcagtttt caccctcgat gccaaccacg
     5641 tcaggtggca aattttcgac attaaccgcg acgatgctaa atttcatgga gaagtgcgtg
     5701 ggtgaaatag ccggtcgtca gtcataaagg gcaggggagt accgttggcc cggcgttcga
     5761 tagtaccgtc cggatactgc cagatatcgg cgtccaggga gatgtcctga agcgcggtgt
     5821 ccggaatttc aggtttgtgt ctctacaaag actaactatc agaaaaactc atcgagcatc
     5881 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     5941 ttctgtaatg aaggagaaaa ttcaccgagg cagttccata ggatggcaag atcctggtat
     6001 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     6061 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     6121 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     6181 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     6241 cgatcgctgt taaaaggaca attactaaca ggaatcgaat gcaaccggcg caggaacgct
     6301 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     6361 gttttcccgg ggagcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     6421 ttgatggtcg gaagaggcat aaatgccgtc agccagttta gtctgaccat ctcatctgta
     6481 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     6541 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     6601 ccatataaat cagcatccag gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     6661 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     6721 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     6781 atttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     6841 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggcc cacctacaat
     6901 aaaggggtcg tttgcgggag agggcgaaat cctacgctaa ggctttggcc aacgatattc
     6961 tccggtaaga ttgatgtgtt cccaggggat aggagaagtc gcttgatatc tagtatgacg
     7021 tctgtcgcac ctgcttgatc gcggccgcga tagctagatc gcgttgctcc tcttctccat
     7081 ccgcgttcca agctgcggaa aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg
     7141 gatcgacgtc cagcgcacga gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga
     7201 gacaaaggtc gtctctgtca gccggatcgt agaacatatt ggcggcgcca aagcccactt
     7261 caccgaccag accgacggga tctatcacca gccagccgcg actggagaac atgatgtttt
     7321 catgatgcag atcgccatgt agcccacgca gttccgaggc attgctcatc atttgatcgg
     7381 ctataatcgc cgcgtggacg tagtcagttt gacaacctgc gttttgatca tcgcgcgccc
     7441 gctgaaacaa agctgcaaag cgatcccgga tcgggagaag ggcagaaggc aggggttcct
     7501 cagatgcggc atacagcttc gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc
     7561 cgtgctcggc aacgatgtga gagagcattc gctccccggc atattcgagc aacatcagat
     7621 tgttctcacg accgagcaac cggactgctc ccctcccatt gcgccatacc agatagtcgg
     7681 ccccgcgcag ttcatcagca atgtcttcta taggtttcaa tcccttgacg attgcaggag
     7741 tcccgtctgg caatgaaact ttccaaacga ggctggaaaa ggtgtccgca atgagaacag
     7801 gttgcgaaac gtgccaatga gcaggaaaaa caggcggcat gaacatcaac cccaagtcag
     7861 agggtccaat cgcagataga aggcaaggcg ttcgcggtcg ggggcttcga tccccaatac
     7921 attgaatagg acagcgaagg cgcgctctgc ttcatctggc gctgcccagt tctcttcggc
     7981 gttagcaatc atgagtgcca aatcggcata gcgatctgct gttccgagcc gcccaaggtc
     8041 gatcagaccc gtgcattgaa gagttttagg gtccaccatg aagttcggca tgcagggatc
     8101 accatggcaa acaaccatat cggtgcgctc ttggtcgagc cgcaccggta gctctcgttc
     8161 gacacgagcc aaaagatcga gctgcggcgt actcttgtcc tcgtccggta agaagtcggg
     8221 attgacggca ttgcgggaca ccacatcaac ggcgcgtccg aacattcgcg acagcctgcg
     8281 ctcaaacgga cattgatcaa ccgataggct gtgaacagcg ccaagttgct gccccattga
     8341 cggccacgct ttgagcaaat ccgctccaga cagatcagcc gccggtactc ccggaattgc
     8401 cgttatcacc aagcatgcac cctcctgttc ctcctgccag ttgatcacct cggggcaagc
     8461 cacacctcga cctttgagcc aaatgaggcg gtcacgctct ccagcgagct caccgcggcg
     8521 ggaagcaggt gcgattttcg cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc
     8581 agattctccg cctctgacag gcaaccagtc agaatgcgat tcacc
//
LOCUS       DAPHDX010000164         8482 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPHDX010000164 DAPHDX010000000
VERSION     DAPHDX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.166"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            300..1277
                     /gene="csiD"
                     /locus_tag="RH058_003934"
     CDS             300..1277
                     /gene="csiD"
                     /locus_tag="RH058_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV3139448.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1297..2565
                     /gene="lhgO"
                     /locus_tag="RH058_003935"
     CDS             1297..2565
                     /gene="lhgO"
                     /locus_tag="RH058_003935"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV3139449.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2588..4036
                     /gene="gabD"
                     /locus_tag="RH058_003936"
     CDS             2588..4036
                     /gene="gabD"
                     /locus_tag="RH058_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3139450.1"
                     /translation="MKLNASNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4050..5330
                     /gene="gabT"
                     /locus_tag="RH058_003937"
     CDS             4050..5330
                     /gene="gabT"
                     /locus_tag="RH058_003937"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV3139451.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5641..7041
                     /gene="gabP"
                     /locus_tag="RH058_003938"
     CDS             5641..7041
                     /gene="gabP"
                     /locus_tag="RH058_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV3139452.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7062..7724
                     /gene="csiR"
                     /locus_tag="RH058_003939"
     CDS             7062..7724
                     /gene="csiR"
                     /locus_tag="RH058_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV3139453.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7725..8174)
                     /gene="kbp"
                     /locus_tag="RH058_003940"
     CDS             complement(7725..8174)
                     /gene="kbp"
                     /locus_tag="RH058_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV3139454.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8258..8416)
                     /locus_tag="RH058_003941"
     CDS             complement(8258..8416)
                     /locus_tag="RH058_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV3139455.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1905 a         2212 c         2378 g         1987 t
ORIGIN      
        1 taatatgttt agtttactaa cggtcatttt gcagtgaagc catttactgt tttttatcga
       61 ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt tattcatttc
      121 ctgtctcact ttgccttaat accctacgtt aaatgttact aatttgttgc ttttgatcac
      181 aataagaaaa caatatgtcg cttttgtgcg catttttcag aaatgtagat atttttagat
      241 tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga
      301 tgaatgcact gaccgccgta cacaataacg ctgtcgattc aggccaggac tatagcggat
      361 tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga
      421 cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat
      481 cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac
      541 cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtgg
      601 gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc
      661 tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga
      721 aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg gaactgcaca
      781 acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa atcgatgagc
      841 aaaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa catctggacc
      901 actatttccg ccatccactg gcgcgtcgcc cgatgcgctt tgccgcgccg ccgagcaaaa
      961 acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt cgcccggtga
     1021 tgcgctatat cgaccagttc gtccagccaa aagattttga agaaggcgtg tggttgagcg
     1081 agctttccga tgccattgaa accagcaaag gcattctttc tgtacccgtt cccgttggca
     1141 aattcctgtt gataaacaac ctgttctggc tgcacggtcg cgaccgcttt actccgcacc
     1201 cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc actcaccact
     1261 accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt gattattggc
     1321 ggcggcatca tcggcatgtc gaccgccatg caactgattg atgtttatcc ggatgcccgc
     1381 attgcgttgc tggaaaaaga gtccggcccg gcctgtcacc agacgggcca caacagcggc
     1441 gtgatccatg ccggggtcta ttacacgccc ggcagcctga aggcgcagtt ttgcctggcg
     1501 ggaaaccgcg ccaccaaagc cttttgcgat caaaacgaca ttcgctatga caactgcggc
     1561 aagatgctgg tcgccacgtc cgatctcgaa atggaacgga tgcgcgcgtt gtgggaacgc
     1621 acggcggcga acggtatcga gcgcgagtgg ttaaacgccg aggaactgcg cgagcgcgaa
     1681 ccgaatatca ccgggctcgg cggcattttt gtgccgtcca gcggcattgt cagctaccgc
     1741 gaagtaacgg cggcgatggc aaaaattttc caggccagag gcggcgagat tatctataac
     1801 gccgaagtca gcggccttag tgagcataaa agcggcgtgg tgatacgtac ccgtcagggc
     1861 ggcgactatg aagcatcgac gctgattagc tgttccgggc tgatggctga ccggctggtg
     1921 aaaatgctcg gcctcgaacc gggctttatc atctgcccgt tccgcggcga gtatttccgc
     1981 ctggcgccgg agcataacca gattgttaac catctgattt accccattcc cgacccggca
     2041 atgccgtttt tgggcgtgca tctcacccga atgatcgatg gcagcgtcac cgtcgggcca
     2101 aacgcggtgc tggctttcaa acgcgaaggc tatcgcaagc gcgacttctc atttagcgac
     2161 acgctggaga ttttgggctc gtcggggatt cgccgggtgc tgcaaaacca tctacgctca
     2221 ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct atctgcgcct ggtgcaaaag
     2281 tattgtcccc ggctttcgtt aagcgatctc cagccctggc ccgccggtgt gcgggcgcag
     2341 gcagtatcgc cggacggcaa gctgattgac gattttctgt ttgtcaccac cccacgcacg
     2401 atccacacct gcaatgcgcc ctccccggca gcgacatcag caattcctat tggtgcgcat
     2461 attgtcagca aggtacaaac gctgttggca agccagagta accccggacg cacgctgcgt
     2521 gcggcacgta gtgtggatgc cttacacgcc gcatttaatc aataaccttt gaaaacagga
     2581 cgtaacaatg aaacttaacg ccagtaactt attccgccag caggcattga ttaacgggga
     2641 atggctggac gccaacaatg gcgaggtcat cgacgtcacc aatccggcga acggcgacaa
     2701 gctgggtagc gtacccaaaa tgggcgctga tgaaacccgc gcggctatcg acgccgccaa
     2761 ccgcgccctg cccgcctggc gcgcgctcac cgccaaagaa cgcgccacca ttctgcgcaa
     2821 ctggttcaat ttgatgatgg agcatcagga cgatttagcg cgcctgatga ccctcgaaca
     2881 gggtaaaccg ctggctgaag cgaaaggcga aatcagctac gccgcctcct ttattgagtg
     2941 gtttgctgaa gaaggcaaac gtatttatgg cgacaccatt cccggtcatc aggccgataa
     3001 acgcctgatt gttatcaagc agccgattgg cgtcactgct gctatcacgc cgtggaactt
     3061 tccggcggcg atgattaccc gtaaagccgg tccggcgctg gcggcaggtt gcaccatggt
     3121 gctgaagccc gccagtcaga cgccgttctc tgcgctggcg ctggcagagc tggcgatccg
     3181 cgcgggcatt ccggctggcg tgtttaacgt ggtcaccggt tcggcgggcg cggtgggtaa
     3241 tgaactgacc agcaacccgc tggtacgcaa actgtcgttt accggctcga ccgaaattgg
     3301 ccgccagtta atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg
     3361 caacgcgccg tttatcgtct ttgacgatgc cgatctcgat aaagccgtgg aaggcgcgct
     3421 ggcctcgaaa ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt tatacgtgca
     3481 ggacggcgtg tatgaccgct ttgccgaaaa actacagcag gcggtgagca aactgcacat
     3541 cggcgacggg ctggataaag gcgtcaccat cgggccgctg atcgatgaaa aagcggtagc
     3601 aaaagtggaa gagcatattg ccgatgcgct ggagaaaggt gcgcgtgtgg tttgcggcgg
     3661 taaagcggat gaacgcggcg gcaacttctt ccagccgacc attctggtgg acgtgccggc
     3721 caacgccaaa gtgtcgaaag aagagacgtt cggccccctc gccccgctgt tccgctttaa
     3781 agatgaagcc gatgtgattg cgcaagccaa tgacaccgag tttggccttg ccgcctattt
     3841 ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt acggcatcgt
     3901 cggcatcaat accggcatta tttccaatga agtggccccg ttcggcggca tcaaagcctc
     3961 gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa tcaaatatat
     4021 gtgcatcggt ctttaactgg agaacgcgaa tgagcagcaa taaagagtta atgcagcgcc
     4081 gcagtcaggc gattccccgt ggcgttgggc aaattcaccc gattttcgct gaccgcgcgg
     4141 aaaactgccg ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg
     4201 cggtgctcaa taccgggcac ctgcatccga aggtggtggc cgcggtggaa gcgcagttga
     4261 aaaaactgtc gcacacctgc ttccaggtgc tggcttacga gccgtatctg gagctgtgcg
     4321 agattatgaa tcagaaggtg ccgggcgatt tcgccaagaa aacgctgctg gttacgaccg
     4381 gttccgaagc ggtggaaaac gcggtaaaaa tcgcccgcgc cgccaccaaa cgtagcggca
     4441 ccatcgcttt tagcggcgcg tatcacgggc gcacgcatta cacgctggcg ctgaccggca
     4501 aggtgaatcc gtactctgcg ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt
     4561 atccttgccc gctgcacggc ataagcgagg atgacgctat cgccagcatc caccggatct
     4621 tcaaaaatga tgccgcgccg gaagatatcg ccgccatcgt gattgagccg gttcagggcg
     4681 aaggcggttt ctacgccgcg tcgccagcct ttatgcagcg tttacgcgcg ctgtgtgacg
     4741 agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcggggcgt accggcacgc
     4801 tgtttgcgat ggagcagatg ggcgttgcgc cggatctcac tacctttgcg aaatcgatcg
     4861 cgggcggctt cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggat gccgtcgctc
     4921 caggcggtct gggcggcacc tatgccggta acccgattgc ctgcgtggcg gcgctggaag
     4981 tgttgaaggt gtttgagcag gaaaatctgc tgcaaaaagc caacgatctg gggcagaagc
     5041 taaaagacgg attgttggcg atagccgaaa aacacccgga gatcggcgac gtacgcgggc
     5101 tgggagcgat gattgccatc gagctgtttg aagatggcga tcacagcaaa ccggacgcaa
     5161 aactcaccgc cgagatcgtg gcacgcgccc gcgataaggg cctgattctt ctctcctgcg
     5221 gcccgtatta caacgtgctg cgcatccttg taccgctcac cattgaagac gctcagatcc
     5281 gtcagggtct ggagatcatc agccagtgtt ttgctgaggc gaagcagtag ctatgtccgt
     5341 ttgtaagagt taaacgttgc taagggaatg ataacgatgc agtaaaaaat aataacgatg
     5401 aggcgatcga caaagttaaa aacgactgcg gactgtcccc tcgctcctct ggggagaggg
     5461 ttagggtgag gggtaatcac gcggctacac cctcaccccg gccctctccc taagggagag
     5521 ggagaaaact ctcccgcttt cgtttttaag attttgttga ccgtctcaat taaacgcgtg
     5581 acccgacgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg
     5641 atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaagtcacg ccacgtcacc
     5701 atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc
     5761 gccgaagcgg gcccggcggt attactggcc tatctgttcg ccggattact ggtggttatg
     5821 attatgcgaa tgctggcgga aatggcggtt gccacgcccg ataccggttc gttttccacc
     5881 tatgccgata aagccattgg ccgctgggcg ggttatacca tcggctggct gtactggtgg
     5941 ttttgggtac tggttatccc gctggaagcc aacatcgccg ctatgatcct gcactcgtgg
     6001 gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt aactggcagt
     6061 aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc
     6121 atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccttat
     6181 gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggcttc
     6241 ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc
     6301 accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc taccaactcg
     6361 gttatctggc gtatttctat cttctatttg tgctctattt ttgtcgtagt ggcgttaata
     6421 ccgtggaata tgccggggct gaaagccgtt ggttcttatc gctcggttct ggaattgctc
     6481 aatattcccc atgcgaaatt aatcatggac tgcgtgatat tactttccgt aaccagctgt
     6541 ctgaactcgg cgctgtatac cgcgtcaagg atgctctact ccttaagccg tcgcggtgat
     6601 gcgcccgcgg taatgggcaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc
     6661 tccaccggag cggcattttt aacggtggtg gtgaactatt acgcacctgc gaaagtgttt
     6721 aaattcctga tagacagctc cggtgctatc gccctgctgg tttatttagt catcgccgtt
     6781 tcacagttgc ggatgcgtaa aattctgcga gcagaaggaa gcgaaattcg cttgcgcatg
     6841 tggctttacc cgtggctcac ctggctggta ataggcttta ttacctttgt gttggtggtg
     6901 atgttattcc gcccggcgca acagttagaa gtgatctcta ccggcttatt agcgataggg
     6961 attatctgta ccgtgccgat tatggcgcgc tggaaaaagc tggtattgtg gcaaaaaaca
     7021 cccgttcata atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg
     7081 atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat
     7141 tacgaatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgtta cgggaagctc
     7201 tttcgcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg
     7261 cgtctatgtc agagcaggag ctgctcgata ttttcgacgc ccgcgccaat atggaagcga
     7321 tgctggtcag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa
     7381 aagcgcatct gctgagtaag ctggaggcct gtgacgccag cgagaaaatg cttgatgagt
     7441 gggatctgcg tcatcaggcg tttcatacag caattgtggc gggctgtggt tctcactatt
     7501 tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ctatcgattt atctggctgc
     7561 ggcgaacggt gctttcggta gaaatgctgg aggataaaca cgatcagcac cagaccctga
     7621 ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc cagcatttac
     7681 tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc ttccggaata
     7741 cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt attcgcttcg
     7801 aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg
     7861 tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt tttcacctga
     7921 tcatcaacac tggcaatacc ggaaatattc cccaccgcaa caaggatttt ctccttcgcc
     7981 tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg aatgttcact
     8041 ttatcggcat caggtatacc ggttttgctc agatgctcct gcaccttctt cgcctgatcg
     8101 tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa
     8161 ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg
     8221 aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt tgcacccaga
     8281 acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg
     8341 cccaaccgaa ccctttaccg agcagcacgc cgagaggcgg cagaatgatg gtgatgacga
     8401 ttctccagaa acccatatgt actccctata agaaaattac tcattgttta aaaagagatt
     8461 ttatctctta agcgtagtaa tt
//
LOCUS       DAPHDX010000165         8385 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPHDX010000165 DAPHDX010000000
VERSION     DAPHDX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.167"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(103..309)
                     /locus_tag="RH058_003942"
     CDS             complement(103..309)
                     /locus_tag="RH058_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV3139456.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            622..711
                     /gene="kdpF"
                     /locus_tag="RH058_003943"
     CDS             622..711
                     /gene="kdpF"
                     /locus_tag="RH058_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV3139457.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            711..2384
                     /gene="kdpA"
                     /locus_tag="RH058_003944"
     CDS             711..2384
                     /gene="kdpA"
                     /locus_tag="RH058_003944"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV3139458.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2407..4455
                     /gene="kdpB"
                     /locus_tag="RH058_003945"
     CDS             2407..4455
                     /gene="kdpB"
                     /locus_tag="RH058_003945"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV3139459.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4464..5036
                     /gene="kdpC"
                     /locus_tag="RH058_003946"
     CDS             4464..5036
                     /gene="kdpC"
                     /locus_tag="RH058_003946"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV3139460.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5029..7713
                     /gene="kdpD"
                     /locus_tag="RH058_003947"
     CDS             5029..7713
                     /gene="kdpD"
                     /locus_tag="RH058_003947"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV3139461.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7710..>8385
                     /gene="kdpE"
                     /locus_tag="RH058_003948"
     CDS             7710..>8385
                     /gene="kdpE"
                     /locus_tag="RH058_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV3139462.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1737 a         2183 c         2453 g         2012 t
ORIGIN      
        1 gattggtata acgatgtcta aatattcctc tgaacatctt cctgatacaa aaaaccgcca
       61 gcgtttccgc tggcggtttt caatgtattc aaaactcagc aatcaataaa aatcaccagt
      121 tgccttttcg gcctgatcca tccacaccgg tttatcgctg gttttcgacc agacgcgatg
      181 caggtagctg taaaagcggg cgcggtcttt ccagaaaagc atgaaaggca gcgccagaac
      241 gcccgctgca accgcataag tacggcgtaa aaagataagc catgcaggat attctctgta
      301 gagttccata cttttctccc ccttttgtat ctacccggtg aatggcaccg gaaaaatgaa
      361 tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat tttactactc atccgaccac
      421 ttatttttgc ttattgatgg tttatttaca ttcatcctgt aattaagtta cacaaaagtt
      481 aaattaatac taaacattag ttaaatcatg gcttttgcca tttttatact ttttttacac
      541 cccgcccgca gatttttgcg aaatctttgc agccagaatt ctacccttcc ggtatcactt
      601 ttaggccact ggaggtgcac tgtgagtgca ggcgtgataa ccggcgtatt gctggtgttt
      661 ttattactgg gttatctggt ttatgccctg atcaatgcgg aggcgttctg atggctgcgc
      721 aagggttctt actgatcgcc acgtttttac tggtgttaat ggtgctggcg cgtcctttag
      781 gcagcgggct ggcgcggctg attaatgaca ttcctcttcc cggtacaacg ggcgttgagc
      841 gcgtactttt tcgcgcactt ggcgtctctg accgtgagat gaactggaag caatatcttt
      901 gtgccattct cggcctgaac atgctggggc tggcggtgct gttttttatg ttgctcggtc
      961 agcactatct gccgcttaat ccacagcagt tgccggggct gtcgtgggat ctggcgctga
     1021 ataccgccgt cagctttgtc acgaatacca actggcaatc ttacagcggt gaaaccacgt
     1081 tgagctattt cagccagatg gctggcttaa cggtgcaaaa ctttctttct gccgccagcg
     1141 ggattgcggt gatttttgcc ctcatccgtg cgtttacccg ccagagcatg agcacactcg
     1201 ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt gctcgtccct gtggcgttgt
     1261 tgatcgcact gttttttatt caacaaggtg cgctgcaaaa ctttcagcct tatcaggctg
     1321 tgaataccgt tgaaggagcg caacagctgt tacccatggg gcctgtagct tctcaggaag
     1381 cgatcaagat gctcggtact aacggcggtg gcttctttaa tgccaactcg tcgcatccgt
     1441 ttgaaaaccc aaccgcactg accaacttcg tgcagatgct ggcgatcttc ttgatcccaa
     1501 cggcgctgtg ctttgccttt ggtgaagtga cgggcgatcg ccgccaggga cgcatgttgc
     1561 tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt ggtgatgtgg gcagaagttc
     1621 agggtaatcc tcatctgctg gcgctcggcg cagacagcag catcaatatg gaaggtaaag
     1681 agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt cgtgactacg gcggcttcct
     1741 gtggcgcggt gattgcgatg catgattcgt ttaccgctct cggtggcatg gtgccgatgt
     1801 ggctgatgca aattggtgaa gtggtgttcg gcggtgtcgg ttctggtctt tacggcatga
     1861 tgctgtttgt cctgctggcg gtgtttattg ccgggctgat gattggtcgt acaccggaat
     1921 atctgggtaa aaaaatcgac gtacgcgaga tgaaactgac tgcactggcg attctggtta
     1981 ccccgacgct ggtgctgatg ggcgcggcgc tggcaatgat gaccgacgcc ggacgtagcg
     2041 ccatgctcaa ccctggcccg catggtttta gcgaagtgct gtacgccgtg tcatccgccg
     2101 ctaacaacaa cggcagcgcc tttgccggat taagcgccaa ctccccgttc tggaactgtt
     2161 tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat tatcccggtg atggcaattg
     2221 ccggttcgct ggtgagtaaa aagagccaac ccgccagctc cggcacgctg ccaacgcacg
     2281 gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct ggttggcgca ctgaccttta
     2341 tccctgccct ggcgcttggt ccggtggcgg aatatctctc ctgatgatat tgagtgagca
     2401 ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg
     2461 aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc gcaatccggt gatgtttatc
     2521 gtctggatcg gcagtctgct gaccacctgt attagcatcg cgatggcaag cggtgcgatg
     2581 cctggcaatg cgctgtttag cgcggcaatt agcggctggc tgtgggtcac cgtactgttc
     2641 gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag cgcaggccaa cagtcttaaa
     2701 ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg
     2761 gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg tgctggtaga agctggcgat
     2821 attatcccct gcgatggtga agtcattgaa ggcggcgcat cggtcgatga aagcgccatc
     2881 accggggaat cggcaccggt gatccgtgaa tccggcggcg attttgcctc cgtcaccggc
     2941 ggcacgcgta ttctttctga ctggctggtg attgagtgta gcgttaaccc cggtgagaca
     3001 tttctggatc ggatgatcgc gatggtggaa ggcgcacagc gacgcaaaac accgaacgag
     3061 attgccctga ctatcctgct gattgctctg accatcgtct ttttactggc aaccgccacg
     3121 ctgtggccgt tttccgcgtg gggcggtaat gccgtgagtg taacggtact ggtggcgctg
     3181 ctggtctgtc tgatcccaac cactattggc ggcctgttgt cagcgatcgg cgtcgccggg
     3241 atgagtcgga tgctgggcgc gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca
     3301 ggtgacgttg acgttctgct actggataaa accggcacca tcacactcgg taaccgtcag
     3361 gcgtcggagt ttatccctgc gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa
     3421 ctggcttcgc tggctgatga aacgccggaa ggccgcagta ttgtgatcct cgccaagcaa
     3481 cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt accgtttact
     3541 gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa aggttctgtc
     3601 gatgccattc gccgccatgt tgaggctaac ggcggtcact tccctaccga tgttgatcaa
     3661 aaagtcgatc aggttgcgcg tcagggggcc acgccgctgg tggtggtgga aggttcccgt
     3721 gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc
     3781 cagctgcgca aaatgggcat taaaacggtg atgattaccg gcgataaccg tctgactgcc
     3841 gccgccattg ctgcggaagc gggcgtcgat gattttctcg ccgaagcgac accggaggcc
     3901 aagctggcat tgattcgtca gtatcaggcg gaaggccgtt tggtggcgat gaccggcgac
     3961 ggcaccaacg atgctccagc gctggcgcag gcagatgtcg cggtggcgat gaactccggt
     4021 actcaggcgg cgaaagaagc gggcaatatg gtcgatctcg actctaaccc gaccaagctg
     4081 atcgaggtgg tacatattgg caaacagatg ctgatgaccc gtggctcgct gaccaccttc
     4141 agcattgcca acgatgtggc gaaatacttc gccattattc cggcggcgtt cgcggcaacg
     4201 tatccgcagt taaatgcgct gaacatcatg cgccttcatt cgcccgactc cgccattctc
     4261 agtgcggtca ttttcaacgc cttgattatc gtctttttga ttcctctggc gttaaaaggc
     4321 gtgagttata aaccgcttac cgcttctgcc atgttgcgcc gtaacttatg gatttacggt
     4381 ctgggtgggc tgctggtgcc gtttattggt atcaaagtca ttgatttact gctgaccgtt
     4441 tgcggtctgg tgtgaggttt accatgcgtg gcttacgtcc ggcattatcg acatttatct
     4501 ttctgttatt gattactggc ggcgtttacc cgctgctgac caccgtgctg gggcaatggt
     4561 ggtttccctg gcaggccaac ggttcgttga ttcgtgaagg tgatacggtg cggggttcgg
     4621 cattaatcgg gcagaatttt accggcaacg gctattttca gggtcgcccg tcggcaacgg
     4681 cagaaatgcc ctataatcca caggcttctg gcgggagcaa tctggcggtc agtaaccctg
     4741 agctggataa acaaatagcc gcacgcgttg ctgcattacg ggccgctaac ccggatgcca
     4801 gtacgaacgt tccggttgaa ctggtgacgg catcggcaag cgggctggac aataatatca
     4861 ccccgcaagc ggcggtctgg caaatcccac gcgtggcgaa agcgcgtaat ctcagcgttg
     4921 aacagctcac gcaactgatc gcaaaataca gccaacaacc gctggtgaaa tatatcggcc
     4981 agccggtcgt caacattgtt gaactcaatc tggcgctgga taaacttgat gaataacgaa
     5041 cccttacgtc ccgaccccga tcgtctgctg gaacaaactg ccgcgccgca tcgggggaag
     5101 ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga cctgggcgat gctggcagaa
     5161 gcccagcgac tgcgggcgca agggctggat attgtggttg gcgtggtaga aacccatggg
     5221 cggaaagata ccgccgccat gcttgaaggg ctggctgttc tgccgcccaa acgccaggcg
     5281 taccgtgggc ggcatatcag cgagtttgat ctcgatgccg ccctcgcccg ccgtccggcg
     5341 ctgatcttaa tggacgaact ggcgcacagt aatgcgccag gttctcgtca tcctaaacgc
     5401 tggcaggata tcgaagaact gctggaagct ggcattgatg ttttcacaac cgtcaacgtt
     5461 cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca ccggaattca ggtacgggaa
     5521 accgtgcccg atcctttttt cgatgccgcc gacgacgtag tgctggtgga cttgcccccg
     5581 gacgatctgc gccagcggct gaaagaaggc aaagtctata ttgccgggca ggcggagcgc
     5641 gccattgaac attttttccg caaaggcaat ctgatcgccc tgcgtgaact ggcactgcgc
     5701 cgtactgccg atcgcgtaga tgagcaaatg cgcgcctggc gggggcatcc tggtgaagag
     5761 aaagtgtggc acacgcgcga cgcgatcctt ttatgcatcg ggcataacac cggcagtgag
     5821 aaactggtcc gcgcggcggc gcggttggcc tcgcgcctgg gtagcgtctg gcacgcggtg
     5881 tatgttgaaa cccctgccct gcatcgctta ccggaaaaaa aacgtcgggc aattctcagc
     5941 gccttacgtc tggcgcagga gctgggcgcg gagacggcta cactttctga tccagcggaa
     6001 gaaaaagcag tagtgcgtta tgcccgtgaa cataatctcg gcaagattat tctcggtcgc
     6061 ccggcctcgc gccgctggtg gcgtcgggaa acgtttgctg accgactggc gcgcatcgcc
     6121 cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac
     6181 gcgccggata gccgctcttt taaagacaag tggcgggtac agattcaggg atgcgtggtt
     6241 gccgccgcgt tatgcgccgt tatcacttta attgccatgc agtggctgat ggcgtttgat
     6301 gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg tggtggcgct attttatgga
     6361 cgctggcctt cggtggttgc taccgtcatt aatgtggtga gtttcgatct cttttttatc
     6421 gccccacgcg gcacgctcgc tgtctctgat gtgcaatatc tgctgacctt cgcggtgatg
     6481 ttaaccgtcg ggctggtgat cgggaacctt actgcgggtg tgcgttatca ggcgcgggta
     6541 gcccgttacc gcgagcaacg cacacggcac ttatatgaaa tgtcgaaagc tctggcggtg
     6601 ggccgcagtc cgcaggacat cgccgccacc agcgaacaat ttattgcctc cacgtttcat
     6661 gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat tgcagccgtt aacacatccg
     6721 caaggaatga cgccatggga cgatgccatc gcgcagtgga gttttgataa aggcctgcct
     6781 gcgggtgcgg gcaccgacac gttacccggc gtgccgtacc agattttgcc gctaaaaagc
     6841 ggcgagaaaa cctacggact ggtggtggtg gaaccgggga atctgcgtca gttgatgatc
     6901 ccggagcagc agcgcctgct agagacgttt acgctgttag tcgccaatgc gtttgagcgg
     6961 ctgacgctga ccgccagtga agaacaggcg cggatggcaa gcgaacgtga acagatccgc
     7021 aacgccctgc tggcggcgct ttcgcatgat ttacgcacgc cgcttacggt gctgtttggt
     7081 caggcagaaa tcttaacgct cgatctggca agcgaaggat cgccccacgc ccgccaggcc
     7141 agcgagattc gtcagcatgt gctgaacact acccgactgg tgaataatct actggatatg
     7201 gcgcgaattc agtccggcgg ctttaatttg aagaaagagt ggttaacgct ggaagaagta
     7261 gtcggcagcg cgctgcaaat gctggaaccg ggtttatcgt cgcccatcaa tctttctctg
     7321 ccagaaccgc tgaccttaat ccacgttgac gggccactct ttgaacgggt gctgattaat
     7381 ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg aaattggtat cgatgcccac
     7441 gttgagggcg aaaatctaca actggatgtc tgggataacg gccccggtct tccgccaggc
     7501 caggagcaga cgatatttga taagtttgct cgcgggaata aagagtcggc agtaccgggg
     7561 gtagggcttg gactggcaat ttgtcgggcg atagtggatg tacacggggg cactattacc
     7621 gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta cacttcccca gcaaaccgct
     7681 cctgaacttg aagactttca tgaggatatg tgacaaacgt tctgattgtt gaagatgaac
     7741 aggctattcg tcgctttctg cgcacggcgc tggagggcga cgggatgcgc gtctatgagg
     7801 ccgaaacgct gcaacgcggc ttgctggaag cggctacccg taagccagat ttgattattc
     7861 tcgatctcgg cctgcccgat ggtgatggga ttgagtttat ccgcgacctg cgccagtgga
     7921 gcgcggtgcc ggtgattgtg ctttccgcac gcagcgaaga gagcgacaaa atcgccgcgc
     7981 tggatgccgg agcggatgat tatctgagta agccgtttgg cattggcgaa ttgcaggccc
     8041 gtctgcgcgt cgctttacgc cgccactccg ccaccgccgc gcccgatccg ctggtgaaat
     8101 tttccgatgt taccgtcgat ttagccgccc gcgtgattca ccggggtgag gaagaggtgc
     8161 atctcacgcc gattgagttc cgcctgctgg cggtgctgct gaataatgcc gggaaagtgc
     8221 tcacccagcg ccagttactc aatcaggtgt gggggccaaa cgcggtcgaa cacagtcact
     8281 atttgcgtat ttatatggga catctgcgac aaaaactgga acaggatccc gctcgccccc
     8341 gccatttcat tactgaaacc ggtattgggt atcggtttat gccat
//
LOCUS       DAPHDX010000166         8227 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPHDX010000166 DAPHDX010000000
VERSION     DAPHDX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.168"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            125..5539
                     /locus_tag="RH058_003949"
     CDS             125..5539
                     /locus_tag="RH058_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HDV3139463.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGSNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGIIQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDMNVRGAL
                     RVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDK
                     MNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTL
                     AKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAAN
                     SLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQL
                     GGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQND
                     ANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTL
                     NTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQ
                     REFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGV
                     QLGDKGYSDTQGMLGVKYSW"
     gene            5748..6014
                     /gene="hipB"
                     /locus_tag="RH058_003950"
     CDS             5748..6014
                     /gene="hipB"
                     /locus_tag="RH058_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV3139464.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6014..7336
                     /gene="hipA"
                     /locus_tag="RH058_003951"
     CDS             6014..7336
                     /gene="hipA"
                     /locus_tag="RH058_003951"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV3139465.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            7648..7856
                     /locus_tag="RH058_003952"
                     /pseudo
     CDS             7648..7856
                     /locus_tag="RH058_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2262 a         1752 c         2222 g         1991 t
ORIGIN      
        1 attgacacct tcccacctcc aactaacact caatacagac ctctgacaat ttaataagtt
       61 tcatttttaa tgtctgacga ctcatctctt tcgtggctgt attgtttgta tatggagcgt
      121 ttatatgaat agaatctatc gcgtgatatg gaattgcact ctacaggtat ttcaggcctg
      181 ctcggaatta actcgcaggg caggtaaaac atcgacggtt aatttgcgta aatcctctgg
      241 actgacaacg aaattcagta gattgacgct gggtgttttg ctggcactaa gcggttcagc
      301 gtctggtgca agtctggaag ttgataatga tcagattacc aatattgata ctgatgttgc
      361 ttatgatgcc tacctggttg gctggtatgg cactggagtg cttaatattt tggctggcgg
      421 taatgcctcc ttaaccacta ttactaccag cgtcattggc gctaatgagg actcagaggg
      481 caccgttaat gttttgggtg gcacctggcg attgtatgat agcggaaata atgcaaggcc
      541 tttaaatgtg ggtcaatccg gaacggggac gctgaatatt aaacagaaag gtcacgttga
      601 tggaggctat ttaagactag gttcctcgac aggaggcgtc gggacggtca atgttgaggg
      661 agaggactct gttctgacga ccgaattatt cgaaataggg agttacggta cgggctcatt
      721 aaatattacg gataagggtt acgtcacgag ttcaatagtc gccattttag gctatcaggc
      781 gggcagtaat ggccaggttg tcgttgaaaa gggcggtgag tggctaataa aaaataacga
      841 ttcctcaatt gaatttcaaa ttggtaatca aggaactggg gaagcgacta ttcgcgaggg
      901 tggactgatt acggctgaaa acacgattat cggtagcaat gctaccggta tcggaaccct
      961 gaatgtgcag gatcaagact ctgtcatcac ggtacgcaga ctctataatg gatatttcgg
     1021 taatggcaca ctcaatattt ccaataatgg actaattaat aacaaagaat attcattagt
     1081 gggcgttcag gacggttccc acggtgtcgt caacgtgacc gataaagggc attggaattt
     1141 cctcggaacg ggcgaagctt tccgctatat ctatatcggt gatgctggcg acggtgaact
     1201 taatgtctcg cgtgaaggta aagtagattc gggaattatc actgcgggga tgaaagaaac
     1261 aggcacaggc aacattactg ttaaggataa gaactccgtt atcactaatc tcggaactaa
     1321 tcttggttat gacggccacg gcgaaatgaa tatcagtaat gaagggcttg ttgtcagcaa
     1381 cggaggaagc tcactcggtt atggagaaac cggcgtcggg aatgtcagca tcaccacggg
     1441 ggggatgtgg gaggtcaata agaatgtcta taccaccatt ggtgttgcgg gcgtcggaaa
     1501 cctcaatatt agcgatggcg gtaagttcgt atcgcaaaat attacttttt tgggcgataa
     1561 agcaagcggt atcggcacac tgaacctgat ggatgcgaca tcatcgttcg atactgtggg
     1621 tatcaatgtc ggtaattttg gtagcggtat cgtaaatgtc agtaatggtg ccacccttaa
     1681 ttcaacgggc tatggattta tcggaggaaa tgcctccggt aaggggatag ttaatatttc
     1741 aacggacagt ctctggaatt taaagacatc atctactaac gcacaattgc tacaggtcgg
     1801 tgtattaggc aagggtgaac tgaatattac caccggaggt atagttaaag cgcgtgatac
     1861 acagatagct ctcaatgaca aaagtaaggg cgacgtgagg gtggatgggc agaactctct
     1921 tcttgaaaca ttcaatatgt acgtagggac atctggtacg ggtacgttaa ccctgacgaa
     1981 tagcggcacg ctgaatgtcg aaggtggaga agtttactta ggtgtttttg aacctgctgt
     2041 aggaacgcta aacattggtg cagctcacgg tgaggcggcg gcagatgccg ggtatataac
     2101 caacgcgacg aaagtagagt ttggttctgg cgaaggtgtt tttgtcttta atcatactaa
     2161 taacagtgat gccggctacc aggtcgatat gctgattaca ggtgacgata aagacggaaa
     2221 agtgatccat gatgcaggcc atactgtgtt caatgcaggg aatacttata gcggtaaaac
     2281 gctggtcaat gacggcctcc tgaccattgc gtctcatacg gcagatgggg taacgggcat
     2341 gggttcgagt gaagtcacca ttgcaagccc cggtacgctc gacattctcg catcaacgaa
     2401 cagtgcagga gattacacgc tgaccaatgc gctcaaaggc gatggcttga tgcgagtgca
     2461 gctgtcatcc tacgacaaga tgtttggctt tacccatgca acagggactg aattcgccgg
     2521 tgttgcccaa ctgaaagaca gtaccttcac tctggaacgc gacaacaccg ctgcgcttac
     2581 tcacgcgatg ttgcagtctg acagtgaaaa taccacatcg gtaaaagtgg gagagcaatc
     2641 cattggtgga ctggccatga atggcggtac gctcattttc gatacggata ttcctgctgc
     2701 gacgcttgca gagggatata tcagcgtcga tacgctggtt gtcggcgcgg gtgactacac
     2761 ctggaaaggc cgtaactatc aggtaaacgg gacgggcgac gtgcttatcg acgtgcctaa
     2821 accgtggaat gatcccatgg cgaataaccc tctgacgacg ctcaatttgc tggaacacga
     2881 cgatagccat gtcggcgttc aactggtgaa ggcgcaaacg gttattgggt cgggtggctc
     2941 attaacgtta cgtgatttac agggcgacga ggtggaagcg gacaaaacgt tacacattgc
     3001 gcaaaacgga acggtggtcg ccgagggtga ttatggattc cgcctcacga ccgcaccagg
     3061 tgatggtttg tacgttaact atgggctgaa agcgctgaac atccatggtg ggcaaaagct
     3121 gacgttagcc gaacatggcg gagcctatgg cgcaacggcc gatatgtcgg caaaaatcgg
     3181 tggtgaaggg gatctggcaa tcaatacggt gcgacaggtt tcgctttcca acggtcagaa
     3241 cgactatcag ggggcaacct acgttcagat ggggacatta cgtaccgatg cggatggtgc
     3301 acttggcaac acccgggaac tgaacatcag caacgcggcc atcgtcgatc ttaatggatc
     3361 gacgcagacg gtagagacat tcaccgggca gatgggttcg actgttttgt tcaaagaggg
     3421 ggcgctgacg gtaaataaag gtgggatcat tcagggtgaa ctgacaggtg gcggaaacct
     3481 gaatgttaca gggggaacgc tggctatcga ggggcttaat gcacgctaca atgcgttaac
     3541 cagcattagc ccaaatgcgg aagtcagcct cgataatact caggggttag gcagaggaaa
     3601 tattgccaat gacggtctgt taacgctaaa aaacgtgact ggcgaactgc gtaatagcat
     3661 aagcgggaag ggtatcgtga gcgcaaccgc caggacagat gtagagctgg atggcgataa
     3721 tagccgcttt gtggggcaat tcaacattga tacaggcagc gcgctcagcg tcaacgagca
     3781 gaaaaacctg ggtgatgctt ccgttatcaa taatggcctg ctcaccatct ccactgagcg
     3841 tagctgggcg atgacgcaca gtatcagcgg tagcggtgat gtgacaaaac tgggtaccgg
     3901 gatcctgact cttaacaacg attccgcggc gtatcagggt acgacggata tcgtgggggg
     3961 ggaaattgct ttcggttccg actctgccat taatatggca agtcaacaca ttaatatcca
     4021 taacagcggt gtgatgtcgg gaaatgtcac cactgcaggt gatatgaacg ttaggggggc
     4081 actgcgtgtc gctaaaacca ctatcggcgg caacctggag aatggcggca cggttcaaat
     4141 gaatagcgaa ggggggaaac cggggaatgt actgaccgtt aacggcaact ataccggaaa
     4201 caatggcctg atgacgttca acgcgacgct gggcggcgat aattcgccca ccgataagat
     4261 gaacgtgaaa ggcgataccc aagggaacac tcgcgttcgg gttgataaca ttggcggcgt
     4321 cggtgcacaa acggtcaacg gtattgaact cattgaggtt ggcggtaatt ctgcaggtaa
     4381 cttcgcgctg accaccggaa ctgtcgaagc tggggcttac gtctacacgc tggctaaagg
     4441 gaaggggaat gacgagaaaa actggtatct gaccagtaaa tgggacggcg taacgccagc
     4501 ggatacaccc gatcccatca ataatccccc tgttgtggat ccggaaggcc catcagttta
     4561 tcgcccggag gccggaagct atatcagcaa cattgccgca gccaactcgc tgtttagcca
     4621 tcgtttacac gaccgtctgg gtgagccgca gtatacagat tcactgcatt ctcaggggtc
     4681 ggcaagcagt atgtggatgc gtcatgtcgg agggcacgaa cgttcaaggg ccggtgacgg
     4741 tcagctaaat actcaggcta accgctatgt attgcagcta ggcggcgatt tggcgcagtg
     4801 gagtagcaac gcgcaggatc gctggcatct tggcgtgatg gcaggttacg ctaatcagca
     4861 cagtaatact cagagtaatc gtgtgggtta taaatcggat gggcgcatca gcggttacag
     4921 cgctgggctg tacgcgacct ggtatcagaa cgatgcgaat aagaccggcg cttatgttga
     4981 cagctgggcg ctgtataact ggtttgataa cagcgtcagt tccgataacc gttctgctga
     5041 cgactatgat tctcgcggtg tgacggcctc tgttgagggt gggtatacct ttgaagcggg
     5101 aacatttagc ggcagcgaag ggacgctgaa tacctggtac gtccagccac aggcgcaaat
     5161 cacctggatg ggtgtgaaag attccgacca tacccggaaa gacggaacgc gcattgaaac
     5221 ggaaggcgac ggaaatgtgc aaacgcgact tggggtgaaa acctacctga acagccatca
     5281 ccagcgtgac gatggtaaac agcgtgagtt ccagccttac attgaagcga attggatcaa
     5341 caacagcaaa gtctacgccg tgaagatgaa tggtcaaacc gtaggccgtg aaggtgcgcg
     5401 taatctcggt gaagtacgta ccggggttga ggcgaaagta aataacaacc ttagcctgtg
     5461 ggggaatgtc ggtgtgcaac taggtgataa aggctatagc gatactcagg gcatgctggg
     5521 agtgaaatat agctggtaaa ccgtataagc cgcatgtcga gatggcatgc ggcttaatat
     5581 tgccgacttc aaaggcttta aagcctccct tttatccgcg atcgcggata tcgcagcgtt
     5641 tatcccgtac agcggataag atgtgtttcc agattgactt tatcctcact aaaggataaa
     5701 actgataata tccccttaag cggataaatt tgctgtggac gtatgacatg atgagctttc
     5761 agaagatcta tagcccaacg caattagcga atgcaatgaa actggttcgc cagcaaaatg
     5821 gctggacgca gagcgagctg gcgaaaaaaa ttggcattaa gcaggcgaca atttccaatt
     5881 tcgaaaacaa ccctgacaat accacgctca cgacattttt taagatttta cagtcgcttg
     5941 aactctcaat gacactatgc gacacgaaaa atgcctcgcc agaatcaaca gaacagcaag
     6001 atctggagtg gtaatgccta aacttgtcac ttggatgaac aaccagcggg taggcgagtt
     6061 aacgaagtta gccaacggcg cgcacacctt taagtatgca ccggagtggt tagccagccg
     6121 ttatgccaga ccgttgtcac tttcgctgcc attgcagagg gggaatatca cctcagatgc
     6181 cgtatttaac ttcttcgata acctgttacc cgatagcccg attgtacgtg accggatcgt
     6241 taaacgttat catgctaaat ccagacaacc gtttgattta ttgtcagaaa tagggcgaga
     6301 cagcgttggt gccgtaacgt tattacccga aaatgaaacc ataacgcgcc cgataatggc
     6361 atgggaaaag cttactgaag ccaggcttga agaagtatta acggcttata aagcagatat
     6421 ccctctgggc atgattagag aagaaaatga ctttcgcatc tcggttgctg gcgcgcagga
     6481 gaagacagcg ctgcttagaa taggcaatga ctggtgcatt ccgaaaggaa taacgccgac
     6541 gacgcacatc attaaattac cgattggcga aatcaggcag cccaatgcga cgctcgatct
     6601 cagccaaagc gttgataatg agtattactg tctgctgctg gcgaaagaac ttgggttgaa
     6661 tgttccggac gcagaaatca ttaaagcggg aaatgtgcgc gcgttagcgg tcgaacgttt
     6721 tgacaggcgt tggaatgctg agcgaacggt tttacttcgc ttgccacagg aggatatgtg
     6781 tcagacattc ggtttacctt catcggtgaa atatgaatca gatggaggcc caggcatcgc
     6841 gcggatcatg gcttttttga tggggtccag cgaggcgctg aaagatcgct atgattttat
     6901 gaaattccag gtcttccagt ggttgattgg cgcaacggac ggtcatgcaa aaaacttctc
     6961 cgtatttatt caggctggcg gcagttatcg actcacgcca ttttacgaca tcatttcagc
     7021 atttccggtc cttggcggta cgggaataca catcagcgat ctcaaactgg caatggggct
     7081 taacgcatcc aaaggcaaaa aaacggcaat cgataaaatt tatccgcgac attttttggc
     7141 gacagcaaag gtgctgagat tcccggaagt gcagatgcat gaaatcctga gtgactttgc
     7201 cagaatgatt ccagcagcac tggataacgt gaagacttca ttaccgacag attttccgga
     7261 gaacgtggtg acggcagttg aaagcaatgt gttgaggttg catggacggt taagccgaga
     7321 atacggtagt aagtgagata tggggtttgg tcattgttaa tgagcatgac aatcatgacc
     7381 gccaacgact ggattcttcc ttggtttata ctggtgcgca taatgtatat tatgttaaat
     7441 catgtctgag tgatgcgaag tgcttttctg gtagtcgtta ttcgttcagg gttcaagaac
     7501 ttaagcctct cctctttcat aattggcctg tgcatggaga aatagcctgg ttattccctg
     7561 ccccatgcta agagcgcagt gtctgtcgca tagacactgc atacgaaaac accagagggt
     7621 gctgttactt atttaaggac ggatcaagcg ggaatgagtc cactccggta tcagaaatgg
     7681 ctacgtctca atgaagtcag gcgaccgatg ctgaatgaac attacgatgt caccacagca
     7741 gcctatgctg tcggttacga aagctatccc atttcagttg ggaatattcg cggatgtttg
     7801 gagagtcacc caagagagat attaccaggt taagaaagtc tgtcggtcag ctttaataaa
     7861 ccctgcactt atctgtttag agcaattcgg tgttagtttc agcaagcaaa cattaaccat
     7921 agctaatgat ttatagccat attaaccatt agtagccctt gacctttcct gacacttccc
     7981 ttgttcgcaa taatattctg tttagtcacg gtttataaat aacatgcata atgaaatcgt
     8041 catgttattt agcgacataa aatgtttttg caaatactct cttcaacaag tgcacaataa
     8101 acttgttgtg attaggtgtc cttgtgattt aaagatatta tcgttcttcg tgaaatgtaa
     8161 taaccctgga aactatattc tcagggcaac aaccaatcaa gaatgattct tgtttttaag
     8221 caataac
//
LOCUS       DAPHDX010000167         8149 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPHDX010000167 DAPHDX010000000
VERSION     DAPHDX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.169"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..833
                     /locus_tag="RH058_003953"
     CDS             <1..833
                     /locus_tag="RH058_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDV3139466.1"
                     /translation="RHRDIIIAKIDLFIQRITHNLWHARKKNVFLIEQITNLKVWVNK
                     YIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKDATPIERCTFDVETKNGLP
                     STVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSA
                     YTDLAGAEKVNINEYNENYLTAQFEELSQRDMKKYLGEMKHLADRGGMKFDDYLDKMS
                     LLVNEGKLEPDILSKMRDAAPKLIDFAKSFDPNSKEKIKILTDTSNLIYDLFGVKSAK
                     "
     gene            1197..2234
                     /gene="dusA"
                     /locus_tag="RH058_003954"
     CDS             1197..2234
                     /gene="dusA"
                     /locus_tag="RH058_003954"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV3139467.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2368..2610
                     /gene="pspG"
                     /locus_tag="RH058_003955"
     CDS             2368..2610
                     /gene="pspG"
                     /locus_tag="RH058_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV3139468.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2776..3759)
                     /locus_tag="RH058_003956"
     CDS             complement(2776..3759)
                     /locus_tag="RH058_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV3139469.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3842..5257
                     /gene="dnaB"
                     /locus_tag="RH058_003957"
     CDS             3842..5257
                     /gene="dnaB"
                     /locus_tag="RH058_003957"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV3139470.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5310..6389
                     /gene="alr"
                     /locus_tag="RH058_003958"
     CDS             5310..6389
                     /gene="alr"
                     /locus_tag="RH058_003958"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV3139471.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6642..7835
                     /gene="tyrB"
                     /locus_tag="RH058_003959"
     CDS             6642..7835
                     /gene="tyrB"
                     /locus_tag="RH058_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV3139472.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2004 a         1910 c         2090 g         2145 t
ORIGIN      
        1 tgcgtcatcg ggatataatt attgcaaaaa tagatttgtt tattcaaaga atcactcata
       61 atttatggca tgcacgtaaa aaaaatgtgt ttttaattga gcaaataact aatctcaaag
      121 tgtgggtcaa taagtatatt gacgattgca ctgatgagga tttaaacgat cgtgacttta
      181 ttgcatcagt tgttgaccgg gctatttttc atttcgcgat taatagtata tgtaatcctg
      241 gggataataa agatgcgact cccattgaac gatgtacttt tgatgtagaa actaagaatg
      301 gccttccctc cacggttcag ctattttatg aggaatctaa ggataatgaa cctttagcga
      361 atatacattt tcaagcaata ggttctggtt ttttaacgtt tgttaatgcc tgccaggaac
      421 atgatgacaa cagcttaaaa ttatttgctt cgctgttaat ttcactttca tattctagtg
      481 cctacacaga tttagccgga gcagaaaaag tgaatattaa tgaatataat gagaactatc
      541 tgacagctca gtttgaagaa ttatctcaac gtgatatgaa gaagtacctg ggagagatga
      601 agcatctggc ggaccggggg gggatgaaat ttgatgacta tctggataaa atgtcacttc
      661 tggtgaatga aggaaagctc gaacctgata ttttaagcaa aatgcgagat gctgcaccga
      721 aattaattga ctttgctaag tcgtttgacc caaactcaaa ggaaaagatt aaaatactta
      781 cagatacttc taatttaatt tatgatttat tcggggttaa atcggcgaaa taatatgtga
      841 agttcttcga tggtatggaa ggcattacat aaaagaacct aatacttatt gggttctttt
      901 ttcttctatc agtgccatta gcaggaagag atatcaccgg agtttaatgt gtgatttttt
      961 atttatcgtc gaacctggat tgtttatcat tgtccttaac aaggctaacg gctaataaga
     1021 ttatttccat cacttcgtga gagcttcatg ccttgatttg atctcaattt tcttttgcaa
     1081 tgagacaggc gcttcctgtt gttatggtat agtaccccgc tattgagcct cctgaacagt
     1141 gatgctgaat aacataaccc aatgatatat cgataaaata atctctacat ttgaaaatgc
     1201 acggtaattc tgaaatgcaa aaaatcaacc aaaccagcgc aatgcctgaa aaaactgacg
     1261 ttcactggag tggtcggttt agcgttgcac caatgctcga ctggacggac agacattgcc
     1321 gctatttctt gcgtctgctt tcccgcaata cgttgctgta taccgaaatg gtgaccacag
     1381 gggcgattat tcacggtaaa ggtgattacc tggcgtacag tgaagaagaa catccggtag
     1441 cgttgcaact cggcggtagc gatccggcgg cgctggcaca gtgtgcgaag ctggcagaag
     1501 cgcgtggata tgatgagatc aacctgaatg tcggctgccc gtctgaccgg gtgcagaacg
     1561 gcatgtttgg tgcgtgtctg atgggtaatg cgcagctggt tgccgactgt gtgaaagcga
     1621 tgcgcgatgt ggtgtcgatt ccagtgacgg tgaaaacgcg tattggcatc gatgaccagg
     1681 acagctatga atttctctgc gatttcatca acactgtttc cggcaaaggc gagtgtgaga
     1741 tgtttatcat ccacgcacgt aaagcctggc tttcggggtt aagcccgaaa gaaaaccgtg
     1801 aaatcccgcc gctcgattat ccgcgtgtgt atcaactgaa gcgtgacttt ccgcatctga
     1861 caatgtcgat taacggtggt atcaagtcgc tggaagaggc caaagcacac ctgcaacata
     1921 tggatggcgt gatggtcggg cgcgaggcgt atcagaatcc gggtattctg gcggcagtag
     1981 accgggagat ctttggttcc tcggataccg atgccgatcc ggtggcggta gtgcgcgcca
     2041 tgtatccgta cattgagcgt gaactcagcc aggggacgta tctcggccat attacccggc
     2101 atatgttggg cttgttccag ggtattcctg gcgcgcggca gtggcggcgt tatttaagtg
     2161 aaaatgccca taaagcgggt gcagacatta atgtgctgga acacgcgctc aaactggtgg
     2221 cggataagcg ttaacttttc accaaaaagt agtcaaattc accacgccct gcgcaccgtc
     2281 gcggggcgtt ttgctgttaa atcaatagat tatttttggc atgattcttg taatgccagc
     2341 aagagatttc atatttggga gagcatcatg ctggaactac tttttgtgat tggctttttt
     2401 gtcatgctga tggtcaccgg cgtttcgttg ctgggcatta tcgccgcgct ggttgtggcg
     2461 acggccatta tgttcctcgg cggtatgctg gcattgatga ttaagttgct gccgtggtta
     2521 ctactggcga ttgcggtggt gtgggttatt aaggcgatta aagcaccaaa agtgccgaaa
     2581 tatcagcgtt atgaccgctg gcgttactaa gggattgtgc ggatgatcac aacctaaggt
     2641 tttatcctta gaacaaaata ggaattgata atcaaatctg tcactattgc gcctctaaca
     2701 gattcatcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg
     2761 gaagccctat ttcttttatg gaatcaacag gctggaacct tgcgtcgccc ggctttccag
     2821 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac
     2881 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc
     2941 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt
     3001 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa
     3061 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta
     3121 cactacgcgc actttcttac cgccggtgat ctctttcaat cgctcgacca gattctcttc
     3181 acgatagtta ataacctgcc acgcgccggc ttttagcgcg ctctgcgctt tttgcgcggt
     3241 tcctacggtg ccgataagtt tcgcgcccag ggcttttgcc cactggcagg caattaagcc
     3301 aacgccgcca gccgctgcgt ggaacaggaa ctgttcatcg ggtttaattt cataggtttt
     3361 gcgcagcaga taataaaccg ttaagccttt caggaaagat gctgcagctt gttcaaaaga
     3421 aattgccgca ggcagaatcg ccgctttatc cgcattaatg ttatgcacag agctgtaagc
     3481 gcctaacgcc gactgcgcat agactacacg atcgcctgcc ttaatatgct ttacaccact
     3541 gccgacttta ctcacgatgc ctgccgcttc ggtacctaat ccgctgggta gtgatggcgg
     3601 cgggtaaagg ccgctgcgga tatacgtgtc gataaaattg atgccgatgg ctttattttc
     3661 gacctggatt tcattctctg ccggatcggc aggagtgaac tctacggctt gaagtacttc
     3721 cgggccaccg tgcttgtgaa attcaattcg tgttgccatg tgtcctccag aacgtatcgt
     3781 cagggtctgc ttcatatggt aaagtttcga cccattcttt atctcggtaa ctccattcac
     3841 tatggcagga aataaaccct tcaacaaaca gcaggctgaa ccccgcgaac gcgatccaca
     3901 agttgccggg ctgaaagtgc ctccgcactc gatcgaagcg gagcagtcgg tgttgggcgg
     3961 tttaatgctg gataacgaac gctgggacga tgtagccgag cgtgtggtgg cagacgattt
     4021 ttacacccgc ccacaccgcc atatctttac tgaaatggcg cgtttgcagg aaagcggtag
     4081 tcctatcgat ctgattaccc ttgcggaatc gctggaacgc caggggcaac tcgatagcgt
     4141 cggtggtttt gcttatctgg cagagctgtc aaaaaatacg ccaagtgcgg cgaacatcag
     4201 tgcttatgct gacatcgtgc gtgaacgtgc cgttgtccgt gagatgatct cggttgcgaa
     4261 tgagattgct gaagccggtt ttgatccgca ggggcgtacc agcgaagatc tgctggacct
     4321 tgctgaatcc cgcgtcttta aaattgccga aagtcgtgca aacaaagacg aagggccgaa
     4381 gaacatcgcc gatgtgctcg acgcaaccgt ggcgcgtatt gagcagttgt ttcagcagcc
     4441 acacgatggc gttaccggag taaacaccgg ttatgacgat ctcaacaaaa aaaccgctgg
     4501 cttgcagccg tcggatttga tcatcgtcgc cgcgcgtccg tcgatgggta aaacaacatt
     4561 tgcgatgaac ctcgtcgaaa acgcggcgat gttgcaggat aaaccagtac ttatcttctc
     4621 gctggagatg ccttcagaac agattatgat gcgttctctg gcgtcgctgt cgcgcgttga
     4681 ccagactaaa atccgtaccg ggcagctcga tgatgaagac tgggcgcgca tttccggcac
     4741 catgggtatt ttgctcgaaa aacgcaatat ctatatcgat gactcctccg gcctgacgcc
     4801 aacggaagtg cgttcccgcg cacgccgtat tgcccgtgaa cacggcggca tcgggcttat
     4861 catgatcgac tacctgcaac tgatgcgcgt accggcgctt tccgataacc gtacgctgga
     4921 aattgcagaa atctcccgct cgctgaaagc actggcgaaa gaactgaacg tgccggtggt
     4981 ggcgctgtcc cagttgaacc gttctctgga acaacgtgcc gacaaacgcc cggtcaactc
     5041 cgacctgcgt gaatctggct ctatcgagca ggatgcggac ttgatcatgt ttatctatcg
     5101 tgatgaggtg tatcacgaaa acagtgattt aaaaggcatc gcggaaatta ttatcggtaa
     5161 acaacgtaac ggcccaatcg ggacggtacg cctgaccttt aacggtcaat ggtcgcgctt
     5221 cgacaactat gcggggccgc agtacgacga cgaataataa ttattttatg aattaggtaa
     5281 ttaaagcaaa cacttatcaa ggaacacaaa tgcaagcggc aactgttgtg attaaccgcc
     5341 gcgctctgcg acacaacctg caacgtcttc gtgaactggc ccctgccagt aaaatggttg
     5401 cggtggtgaa agcgaacgct tatggtcacg gtcttcttga gaccgcgcga acgctccccg
     5461 atgctgacgc ctttggcgta gcccgtctcg aagaagctct gcgactgcgt gcggggggaa
     5521 tcaccaaacc tgtactgtta ctcgaaggct tttttgatgc cagagatctg ccgacgattt
     5581 ctgcgcaaca ttttcatacc gccgtgcata acgaagaaca gctggctgcg ctggaagagg
     5641 ctagcctgga cgagccggtt accgtctgga tgaaactcga taccggtatg caccgtctgg
     5701 gcgtaaggcc ggaacaggct gaggcgtttt atcatcgcct gacccagtgc aaaaacgttc
     5761 gtcagccggt gaatatcgtc agccattttg cgcgcgcgga tgaaccaaaa tgtggcgcaa
     5821 ccgagaaaca actcgctatc tttaatacct tttgcgaagg caaacctggt caacgttcca
     5881 ttgccgcatc gggtggcatt ctgctgtggc cacagtcgca ttttgactgg gtgcgcccgg
     5941 gcatcattct ttatggcgtc tcgccgctgg aagatcgctc caccggtgcc gattttggct
     6001 gtcagccagt gatgtcacta acctccagcc tgattgccgt gcgtgagcat aaagccggag
     6061 aacctgttgg ttatggtgga acctgggtaa gcgaacgtga tacccgtctt ggcgtagtcg
     6121 cgatgggcta tggcgatggt tatccgcgcg ccgcgccgtc cggtacgcca gtgctggtga
     6181 acggtcgcga agtaccgatt gtcgggcgcg tggcgatgga tatgatctgc gtagacttag
     6241 gtccacaagc gcaggacaaa gccggggatc cggtcatttt atggggcgaa ggtttgcccg
     6301 tagaacgtat cgctgaaatg acgaaagtaa gcgcttacga acttattacg cgcctgactt
     6361 caagggtcgc gatgaaatac gtggattaat cgttctgtaa tatttgattg tctgtgccgg
     6421 atgcggcgtg aatgccttat ccggccaata aaatcctaaa aattcaataa gttgatgttc
     6481 tttcatgctc ttataaaggt cgtgcctctg gcggatgtac gtttgtcatg agtctcactc
     6541 tgttgctaat tgccgttcgc tcctgaacat ccactcgatc ttcgccttct tccggtttat
     6601 tgtgttttaa ccacctgccc gtaaacctgg agaaccatcg cgtgtttcaa aaagttgacg
     6661 cctacgctgg cgacccgatt cttacgctta tggagcgttt taaagaagac cctcgcagcg
     6721 acaaagtgaa tttaagtatc ggtctgtact acaacgaaga cggaattatt ccacaactgc
     6781 aagccgtggc ggaggcggaa gcgcgcctga atgcgcagcc tcatggcgct tcgctttatt
     6841 taccgatgga agggcttaac agctatcgcc atgccattgc gccgctgcta tttggtgcgg
     6901 accatccagt actgcaacaa cagcgcgtag caaccattca aacccttggc ggctcagggg
     6961 cattgaaagt gggcgcggat ttcctgaaac gctacttccc ggaatcaggc gtctgggtca
     7021 gcgatcctac ctgggaaaac cacgtagcaa tattcgccgg ggctggattc gaagtgagta
     7081 cttacccctg gtatgacgaa gcgactaacg gcgtgcgctt taatgacctg ttggcgacgc
     7141 tgaaaacatt acctgcccgc agtattgtgt tgctgcatcc atgttgccac aacccaacgg
     7201 gtgccgatct cactaatgat cagtgggatg cggtgattga aattctcaaa gcccgcgagc
     7261 ttattccatt cctcgatatt gcctatcaag gatttggtgc cggtatggaa gaggatgcct
     7321 acgctattcg cgccattgcc agcgctggat tacccgctct ggtgagcaat tcgttctcga
     7381 aaattttctc cctttacggc gagcgcgtcg gcggactttc tgttctgtgt gaagatgccg
     7441 aagccgctgg ccgcgtactg gggcaattga aagcaacagt tcgccgcaac tactccagcc
     7501 cgccgaattt tggtgcgcag gtggtggctg cagtgctgaa tgacgaggca ttgaaagcca
     7561 gctggctggc ggaagtagaa gagatgcgta ctcgcattct ggcaatgcgt caggaattgg
     7621 tgaaggtatt aagcacagag atgccagaac gcaatttcga ttatctgctt aatcagcgcg
     7681 gcatgttcag ttataccggt ttaagtgccg ctcaggttga ccgactacgt gaagaatttg
     7741 gtgtctatct catcgccagc ggtcgcatgt gtgtcgccgg gttaaatacg gcaaatgtac
     7801 aacgtgtggc aaaggcgttt gctgcggtga tgtaatgcag gaaagcaggc tggagctacc
     7861 cagcctgcag tgaaattaaa ctgtcgtcgc tttcactctt tctttataga tgattttttt
     7921 gatgccatcg ttctacgtga gagataataa acgttattag ttcttttatt gttaagctta
     7981 tcctaattat ctggaattcc ttatcctgtt tttttgggtg gagtaataat ttctgcacat
     8041 ctaactatta ttaaatttaa taataagtat cgggtttgtt acgatagaat ttttctatat
     8101 cctcaagaat atgctttgcc ttattgcctg tgactttctg agattcaat
//
LOCUS       DAPHDX010000168         8102 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPHDX010000168 DAPHDX010000000
VERSION     DAPHDX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.170"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            38..1762
                     /gene="iucA"
                     /locus_tag="RH058_003960"
     CDS             38..1762
                     /gene="iucA"
                     /locus_tag="RH058_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDV3139473.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1763..2710
                     /gene="iucB"
                     /locus_tag="RH058_003961"
     CDS             1763..2710
                     /gene="iucB"
                     /locus_tag="RH058_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDV3139474.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2710..4452
                     /gene="iucC"
                     /locus_tag="RH058_003962"
     CDS             2710..4452
                     /gene="iucC"
                     /locus_tag="RH058_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDV3139475.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4449..5726
                     /gene="iucD"
                     /locus_tag="RH058_003963"
     CDS             4449..5726
                     /gene="iucD"
                     /locus_tag="RH058_003963"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDV3139476.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            5808..8009
                     /gene="iutA"
                     /locus_tag="RH058_003964"
     CDS             5808..8009
                     /gene="iutA"
                     /locus_tag="RH058_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDV3139477.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1878 a         2138 c         2167 g         1919 t
ORIGIN      
        1 aattgttatt attttactgt gtaggagctg tttgattatg atcctgccct ctgaaaaatc
       61 cgccacagat gtggctgcgc agtgcttcct gaatgccttg attcgtgaaa ccaaagactg
      121 gcaactggcg gaatacccgc cagacgaatt gattatcccg ctggacgaac agaagtcgct
      181 ccatttcaga gtggcttatt tctccccaac ccagcatcac cgctttgcat ttcctgctca
      241 tctggtcacg gcatcaggca gttatcctgt cgactttacc actctctccc ggcttattat
      301 tgataagctc cggcatcaac tttttctgcc agttcccctc tgcgaaactt tccaccaacg
      361 cgtgctggaa agctacgccc atacgcaaca gacaattgat gcccgccatg actgggccat
      421 cctgcgtgaa aaagcgttga attttggcga ggctgagcag gcactgctga caggacacgc
      481 tttccaccct gcacctaagt ctcatgaacc gtttaaccgc caggaagctg aacgatacct
      541 gcctgacatg gcacctcact tcccgctgcg gtggttttcg gtggataaaa cgcaaatcgc
      601 tggtgaaagt ctgcatctta accttcaaca gcggttgacg cgatttgccg cagaaaatgc
      661 gccccagtta ctcaacgaat taagtgacaa tcaatggctg ttcccgctgc acccgtggca
      721 gggagaatat cttttgcagc aagtgtggtg ccaggcactt tttgctaaag gacttatcag
      781 agacttaggc gaggccggca cgtcgtggct gccgaccacc tcttcccgct ccctctactg
      841 tgctaccagc cgcgatatga tcaagttctc cctgagcgtt cggctgacca actccgtccg
      901 tactctgtct gtgaaagagg tggagcgagg aatgcgcctg gcacgtctgg cgcaaaccga
      961 cggctggcag atgctacagg cccgcttccc tactttccgg gtaatgcagg aggacggctg
     1021 ggccgggctg cgcgatctta acggcaacat catgcaggaa agtttgtttt ccctgcgtga
     1081 aaacctgctg ctggagcagc cgcaaagcca gaccaacgtt ctggtttccc tgactcaggc
     1141 cgcaccggat ggcggtgatt cgctgctggt ttcggcggta aaacgcctga gcgatcgcct
     1201 cggtatcact gtgcaacagg ccgcccacgc atgggtcgat gcttactgtc agcaggtgct
     1261 gaagcctctg tttacggctg aagcggatta cggcctggtg ctgctggcgc atcagcaaaa
     1321 tattcttgtg cagatgcttg gggatctgcc ggtcggattt atttaccgtg actgccaggg
     1381 cagcgcgttt atgcctcacg cgacagagtg gctcgatacc attgacgagg cgcaggcgga
     1441 aaatattttc acccgtgagc agttgctgcg ctatttccct tattacctgc tggttaactc
     1501 cacttttgcc gtaactgctg cgctgggtgc tgccgggctg gacagcgaag cgaatctgat
     1561 ggctcgtgta cgaacattgc tggctgaagt gcgtgaccag gtgactcata aaacctgtct
     1621 caactatgtt ctggaaagtc cgtactggaa cgtaaaaggt aactttttct gttatctgaa
     1681 cgatcataac gagaacacca tcgttgaccc ttcggtgatc tattttgatt tcgctaaccc
     1741 gctgcaggct caggaggtct gaatgtctgg ggcaaacatt gttcacagcg gatatggact
     1801 gcgctgtgaa aaactcgaca agcctctgaa tcttggctgg gggctggaca atagcgcggt
     1861 gttgcactgg cccggggagc tgccaacagg gtggctgtgc gacgcgctgg atcagatatt
     1921 tatcgccgca ccacaacttt cagcagtggt tcttccctgg tccgaatggt gtgaggagcc
     1981 acaggcgctg acgcttttcg gacaggtaca aagcgacatt atccatcgtt ccgctttctg
     2041 gcagttaccg ttatggctga gttctccggc aaaccgggcc tccggtgaaa tggtttttga
     2101 tgcagagcgt gagatttatt tcccgcagcg ccccccccgt ccgcagggtg aagtttatcg
     2161 tcgttacgat ccacgcattc gcaggatgct gagtttccgc attgccgatc ccgtttctga
     2221 tgcagaacgt ttcactcgct ggatgaacga tccgcgcgtt gagtatttct gggagcaaag
     2281 tggctcactg gaggtacaga tcgcctatct ggaacgccag ctcaccagta aacatgcatt
     2341 cccgctgatt ggttgtttcg acgatcggcc gttcagttat ttcgaaatct actgggcggc
     2401 ggaagaccgc attggtcgcc actattcgtg gcagcccttt gaccgtggcc tgcatctgct
     2461 ggttggtgaa cagcaatggc gcggagccca ctatgtgcaa agctggctgc gcggggtgac
     2521 acattacctg ctgctgaatg agccacgtac gcagcgcaca gtactggagc cacgcaccga
     2581 taaccagcgc ctgttccgcc atcttgagcc tgcgggatac cggacaatta aagagttcga
     2641 cttcccacat aaacgctcgc gcatggtgat ggcggatcgt catcacttct tcacggaggt
     2701 cggtctgtaa tgaatcacaa agactgggat ttggtcaacc gccgcctggt ggcaaaaatg
     2761 ttgtctgagc tggagtatga gcaggttttc cacgcggaat ctcagggcga tgaccgctac
     2821 tgcattaacc tgccgggagc acaatggcgc ttcatcgctg aacgtggtat ctggggctgg
     2881 ctctggattg atgctcaaac tctgcgctgc gcggacgagc cagtactggc tcagacgctg
     2941 ctgatgcagc taaagcaggt actgtcaatg agcgatgcaa ccgttgctga gcatatgcag
     3001 gatttgtatg ccacgctgct gggcgacctg caactactga aagcccgtcg cgggctgagc
     3061 gccagtgacc tgattaatct taatgccgac cgcctgcaat gcctgctgag cggtcatcct
     3121 aaattcgttt ttaataaagg tcgccgtggc tggggtaaag aggcgctgga acgatatgcg
     3181 ccagagtatg ccaacacctt cagactgcac tggctggcgg taaaacgtga acatatgatc
     3241 tggcgctgtg ataacgagat ggatattcat cagttgttga cggccgcaat ggatccgcag
     3301 gagtttgccc gcttcagtca ggtctggcag gaaaacggac tggatcataa ctggctgccg
     3361 ctgccggtac atccgtggca gtggcagcaa aaaatcgcta ccgacttcat cgctgatttt
     3421 gccgaaggca ggatggtgtc tctcggcgag tttggcgacc agtggctcgc ccagcagtcg
     3481 ctgcgtaccc tgaccaacgc cagtcgccgg ggcgggctgg atatcaagct gccgctgacc
     3541 atctacaaca cctcatgcta ccgggggatc cctggcagat acatcgctgc cggaccactg
     3601 gcttcacgct ggctacaaca ggtttttgcg accgacgcca ccctagtgca aagcggcgca
     3661 gtgatacttg gtgaaccggc tgcaggctat gtctcccatg aaggctatgc cgcgcttgcc
     3721 cgggctccct atcgctacca ggaaatgctt ggtgttatct ggcgggagaa tccgtgccgc
     3781 tggctgaaac cggatgaaag tccggttctg atggcaacac tgatggagtg cgacgaaaac
     3841 aatcagccgc tggcaggcgc atatatagac cgctccgggc tggacgctga aacctggctt
     3901 acgcaactgt tccgggtggt cgtggttcct ctgtatcacc tgctttgccg ctacggtgtc
     3961 gcgcttattg cacatggaca aaatataact ctcgccatga aagagggggt tccacagcgt
     4021 gttctgctga aagacttcca gggcgatatg cgactggtga aagaagagtt ccccgaaatg
     4081 gactctttgc ctcaggaggt tcgtgatgtt acatcccgcc tgagtgcgga ctacttaatc
     4141 catgatttgc agacgggtca cttcgtgaca gtactgcgtt ttatttcgcc actgatggtt
     4201 cgtcttggcg tacctgaaag gcgattttat caactgctgg cagcagtgtt gagtgattac
     4261 atgaaaaaac atccacaaat gtcagagcgt tttgcgcttt tctcactttt caggccacaa
     4321 atcattcgcg tggtgctgaa cccggtaaaa ctgacctggc ctgatctgga tggcggcagc
     4381 cgcatgctgc cgaattacct tgaggatctg caaaatccgc tgtggctggt aactcaggaa
     4441 tatgaatcat gaaaaaaagt gtcgatttta ttggtgtagg gacagggcca tttaatctca
     4501 gcattgctgc gttgtcacat cagatcgaag aactggactg tctcttcttt gatgaacatc
     4561 ctcatttttc ctggcatccg ggtatgctgg taccggattg tcatatgcag accgtctttc
     4621 tgaaagatct ggtcagtgct gttgcaccta caaatcccta cagttttgtt aactatctgg
     4681 tgaagcacaa aaagttctat cgcttcctta caagcagact acgtacagta tcccgtgaag
     4741 agttttctga ctacctccgc tgggctgctg aagatatgaa taacctgtat ttcagtcata
     4801 ccgttgaaaa cattgatttc gataaaaaac gtcgattgtt tctggtgcaa accagccagg
     4861 gagaatattt tgcccgcaat atctgccttg gtacaggaaa acaaccttat ttaccaccct
     4921 gtgtgaagca tatgacacaa tcctgtttcc atgccagtga aatgaatctt cgtcggccgg
     4981 accttagtgg aaaacggata accgtggttg gtggaggaca gagtggtgca gacctgttcc
     5041 ttaatgcatt acgcggggaa tggggagaag cggcggaaat aaactgggtc tcccggcgta
     5101 ataattttaa cgcactggat gaggctgctt ttgctgatga gtattttaca cctgaatata
     5161 tttcaggctt ctccggactg gaggaagata ttcgccatca gttactggat gagcagaaaa
     5221 tgacatcgga tggcatcact gccgattctt tactgaccat ttatcgtgag ttgtaccacc
     5281 gttttgaagt tctgagaaaa ccaagaaata tccgtctgct acccagccgc tcggtaacaa
     5341 ctctggaaag tagtggtcct ggctggaagt tgctgatgga gcatcatctg gatcagggca
     5401 gggagagcct ggaaagtgat gtggtgattt tcgccacagg ttaccgttct gcgttgccac
     5461 aaatacttcc ctcactgatg cccctgatca ccatgcacga taagaacacc tttaaagtgc
     5521 gtgatgactt cactctggaa tggagtggcc cgaaagagaa caacatcttc gtggtcaacg
     5581 ccagtatgca aacccatggc atcgccgaac cccagctcag cctgatggca tggagatctg
     5641 cacgtattct taatcgcgta atgggacgtg atttattcga tctcagtatg ccgcccgccc
     5701 tgattcagtg gcgcagcggc acctagggaa aacgcagccg gaggctgctt ctttaactcg
     5761 ctacacagca tctttgggct gattttttcc gcccgtatgg aggaataatg atgataagca
     5821 aaaagtatac gctttgggct ctcaacccac tgcttcttac catgatggcg ccagcagtcg
     5881 ctcaacaaac cgatgatgaa acgttcgtgg tgtctgccaa ccgcagcaat cgcaccgtag
     5941 cggagatggc gcaaaccacc tgggttatcg aaaacgccga actggaacag cagattcagg
     6001 gcggcaaaga gcttaaagac gcactggctc agctgatccc tggccttgac gtcagcagcc
     6061 ggagccgcac caactacggt atgaatgtgc gtggccgccc gctggtcgtg ctggttgacg
     6121 gcgtgcgtct caactcttca cgtaccgaca gccgacaact ggactctata gatcctttta
     6181 atatcgacca tattgaagtg atctccggtg cgacgtccct gtacggcggc ggcagtaccg
     6241 gtggcctgat caacatcgtg accaaaaaag gccagccgga aaccatgatg gagtttgagg
     6301 ctggcaccaa aagtggcttt agcagcagta aagatcacga tgaacgcatt gccggagctg
     6361 tctccggcgg aaatgagcat atctccggac gtctttccgt ggcatatcag aaatttggcg
     6421 gctggtttga cggtaacggc gatgccacct tgcttgataa cacccagacc ggcctgcagt
     6481 actccgatcg gctggacatc atgggaactg gtacgctgaa catcgatgaa tcccggcagc
     6541 ttcagttgat cacacagtac tataaaagcc agggcgacga cgattacggg cttaatctcg
     6601 ggaaaggctt ctctgccatc agagggacca gcacgccatt cgtcagtaac gggctgaatt
     6661 ccgaccgtat tcccggcact gagcggcatt tgatcagcct gcagtactct gacagcgctt
     6721 ttctgggaca ggagctggtc ggtcaggttt actaccgcga tgagtcgttg cgattctacc
     6781 cgttcccgac ggtaaatgcg aacaaacagg tgacggcttt ctcttcgtca cagcaggaca
     6841 ccgaccagta cggcatgaaa ctgactctga acagcaaacc gatggacggc tggcaaatca
     6901 cctgggggct ggatgctgat catgagcgct ttacctccaa ccagatgttc ttcgacctgg
     6961 ctcaggcaag cgcttccgga gggctgaaca acaagaagat ttacaccacc gggcgctatc
     7021 cgtcgtatga catcaccaac ctggcggcct tcctgcaatc aggctatgac atcaataatc
     7081 tctttaccct caacggtggc gtacgctatc agtacactga aaacaagatt gatgatttca
     7141 tcggctacgc gcagcaacgg cagattgccg ccgggaaggc tacatccgcc gacgccattc
     7201 ctggcggctc agtcgattac gacaacttcc tgttcaacgc cggtctgctg atgcacatca
     7261 ccgaacgcca gcaggcatgg ctcaacttct cccagggcgt ggagctgccg gacccgggta
     7321 aatactatgg tcgcggcatc tatggtgctg cagtgaacgg ccatcttcct ctaacaaaga
     7381 gtgtgaacgt cagcgacagc aagctggaag gcgtgaaagt cgattcttat gagctgggct
     7441 ggcgctttac tggcaataat ctgcgtaccc aaatcgcggc ctactattcg atttctgata
     7501 agagcgtggt ggcgaataaa gatctgacca tcagcgtggt ggacgacaaa cgccgtattt
     7561 acggcgtgga aggtgcggtg gactacctga ttcctgatac tgactggagt accggagtga
     7621 acttcaacgt gctgaaaact gagtcgaaag tgaacggtac ctggcagaaa tacgatgtga
     7681 agacagcaag cccatcaaaa gcgacagcct acattggctg ggcaccggac ccgtggagtc
     7741 tgcgcgtgca gagcaccacc tcctttgacg tgagcgacgc gcagggctac aaggtcgatg
     7801 gctataccac cgtggatctg ctcggcagtt atcagcttcc ggtgggtaca ctcagcttca
     7861 gcattgaaaa cctcttcgac cgtgactaca ccactgtctg ggggcagcgt gcaccactgt
     7921 actacagccc gggttacggc ccagcgtcac tgtacgacta caaaggcagg ggccgaacct
     7981 ttggtctgaa ctactctgtg ctgttctgac cggtattcct ttacaacaaa ggtacgctga
     8041 tatcaacatg gccgctgaca gccaagttga tatcatataa tacacgacat aatctgtagt
     8101 ca
//
LOCUS       DAPHDX010000169         8097 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPHDX010000169 DAPHDX010000000
VERSION     DAPHDX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.171"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..246
                     /locus_tag="RH058_003965"
     CDS             <1..246
                     /locus_tag="RH058_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139478.1"
                     /translation="LFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLP
                     VNLTQETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            469..966
                     /gene="ubiC"
                     /locus_tag="RH058_003966"
     CDS             469..966
                     /gene="ubiC"
                     /locus_tag="RH058_003966"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV3139479.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            979..1851
                     /gene="ubiA"
                     /locus_tag="RH058_003967"
     CDS             979..1851
                     /gene="ubiA"
                     /locus_tag="RH058_003967"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV3139480.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2006..4429)
                     /gene="plsB"
                     /locus_tag="RH058_003968"
     CDS             complement(2006..4429)
                     /gene="plsB"
                     /locus_tag="RH058_003968"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV3139481.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4600..4968
                     /gene="dgkA"
                     /locus_tag="RH058_003969"
     CDS             4600..4968
                     /gene="dgkA"
                     /locus_tag="RH058_003969"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV3139482.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5078..5686
                     /gene="lexA"
                     /locus_tag="RH058_003970"
     CDS             5078..5686
                     /gene="lexA"
                     /locus_tag="RH058_003970"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV3139483.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5759..7084
                     /gene="dinF"
                     /locus_tag="RH058_003971"
     CDS             5759..7084
                     /gene="dinF"
                     /locus_tag="RH058_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV3139484.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGVWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7200..7409
                     /locus_tag="RH058_003972"
     CDS             7200..7409
                     /locus_tag="RH058_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV3139485.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7451..7966)
                     /gene="zur"
                     /locus_tag="RH058_003973"
     CDS             complement(7451..7966)
                     /gene="zur"
                     /locus_tag="RH058_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV3139486.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1808 a         1990 c         2235 g         2064 t
ORIGIN      
        1 ctgttctcct ctgtcgatga tgggcctgtt atggtggtaa gctcccagcg cttacatgat
       61 atgttgaatc ctacaaaaga taccaattgg aattccacgt atatctacaa atccagacat
      121 gagatgttgc ctgttaatct tactcaggaa acacttttca gctccaaatc tcatggtaaa
      181 tatgcgcttt tccccatttt tactgcgagt tggcgagctc atcgtataat gaataagggt
      241 gtttaagtaa aggaaaacat caccgttcct ggcatcctgg gcggtgatgc ccctatggtt
      301 gccctctcca gcgcgggcat cggcaaagcg taaggttcaa cagcgtttta ccacttcatg
      361 cgattgttgc gtttttgttg cgtattagat cacttaattt gctttacatc tcccgtaaac
      421 acttttctgc gatacaatgc ctttacgtta tgtaacggag agttcggcat gtcacacccc
      481 gcgttaacgc aactgcgtgc gctgcgctat tttaaagaga tccctgccct ggaaccgcaa
      541 ctgctcgact ggctgttgct ggaggattcc atgacaaaac gttttgaaca gcagggaaaa
      601 acggtaagcg tgacgatgat ccgcgaaggg tttgtcgagc agaatgaaat ccccgaagaa
      661 ctgccgctgc tgccgaaaga gtctcgttac tggttacgtg aaattttgtt atgtgccgat
      721 ggtgaaccgt ggcttgccgg tcgtaccgtc gttcctgtgt caacgttaag cgggccggag
      781 ctggcgttac aaaaattggg taaaacgccg ttaggacgct atctgttcac atcatcgaca
      841 ttaacccggg actttattga gataggccgt gatgccggac tgtgggggcg acgttcccgc
      901 ctgcgattaa gcggtaaacc gctgttgcta acagaactgt ttttaccggc gtcaccgttg
      961 tactaagagg aaaaaaatat ggagtggagt ctgacgcaga ataagctgct ggcgtttcat
     1021 cgcttaatgc gtacggataa gccaattggc gcgttactgc tgctctggcc aacattatgg
     1081 gcgttgtggg tggcgacacc gggcgttcct cagctctgga tcctggcggt atttgtcgcg
     1141 ggtgtctggc tgatgcgcgc tgctggatgt gtggtgaatg attatgctga ccgaaagttt
     1201 gatggtcatg ttaagcgcac ggcgaaccga ccacttccca gcggcgcggt aacagagaaa
     1261 gaggcgcgcg cgctgtttgt agtgctggta ctgatttcgt ttttactggt actgacgctg
     1321 aatacgatga ccattctgtt gtcgattgcc gctctggcgt tggcgtgggt gtacccgttt
     1381 atgaagcggt atacccatct cccgcaagtg gtgctgggcg cggcgtttgg ctggtcgatt
     1441 ccaatggctt ttgccgctgt gagtgagtcg gtgccactga gttgctggtt aatgttcctc
     1501 gccaatattc tctgggcggt ggcttacgac acgcagtatg cgatggttga ccgcgatgat
     1561 gatgtgaaga ttggcattaa atccacggca atcctgttcg gccaatacga taaattgatt
     1621 attggtattt tgcagattgg cgtactggca ctgatggcga tcatcggtga gttaaatggc
     1681 ttaggctggg gatattactg gtcaattgtg gtggctggcg cgctgtttgt ttatcaacaa
     1741 aaactgattg ccaaccgcga gcgtgaagcc tgctttaaag catttatgaa taataactat
     1801 gttggtctgg tactgttttt aggactggca atgagttact ggcatttctg atgatgtaaa
     1861 aaagccggat gatcatccgg ctttcttctg ggttgcctta tgcgcggcgc ttctcaggcc
     1921 tacacaacac atcgcaattt attgaatttg cagattatgg aaggccggat aaggcgtttt
     1981 cgccgcatcc ggcaattctc tctgattacc cttcgccctg cgtcgcactc tcaatcgtca
     2041 aacgcacgtc tgatgtaatc aactccgcca gcaactgata aaccttcatc gtttctgccg
     2101 gttcggcgtc gccgctatcg ctgatatacc cttcatcacg cagcgtcagc accagagaac
     2161 tgaacaccgc cttgtcgaag aactccggcg cgttgatgcc gtgcagcacg gagagacgtt
     2221 gcgcgacggt gcggctctct ttctccagcg taccgcggtt gatcgacggg ttggcactca
     2281 acaaccagaa ggtgatggca taacgttgca gcgtttcgcg cgcgcctgcg gccagcagct
     2341 gtagggtgcg agaatgcgcc gggttgatat gcaactcatc atcttgcagg gtaatcagcc
     2401 cctgacgttg catctcattt gccagcgcat caataacgtc cggcaactcg tcgcgatccc
     2461 agcgcaggaa cagctccgct ttcagcattg ggtaaagcac attgacgtgc tccatcaata
     2521 cgtcgcggga gatgtggcga tgctgggtga caattgccgc catcagcgaa ggcagcacca
     2581 acatatgcgc aatgttgttg cgatagtagg tcatcagcac cgcttgctcg cgtggcagaa
     2641 tgatgatgtc gccgatagtg tctttctcga cttcaaactt gttcatttgc agcgcgtgat
     2701 cgataagctc gctggcgctg gctgaaggaa cggtagagtc cgtggagtaa ggtacgttgc
     2761 gcatcagatc caggtagcag ttgagttgct cggttaactg ctcgcgggtg agtgagcgct
     2821 gacgtgatgc cagaagcgca gtacaacaca ggttcatggc gtttgccgcg cctgcgttgt
     2881 taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac catgccggac
     2941 gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg ttaaggtagg
     3001 tcatcaacgg cattggttca ccgaagttga cgtaaccctg accgagatta cgcagcttgc
     3061 ttaaaccgcg cagcatctgc ggcaggctct ctttctcttt cgtcgcgccg cgcagttctt
     3121 tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga atcagcgtaa
     3181 tcggacgcgt gccgccacgc agcatcgcct gaatggtcat cgacagcgta ccggttttcg
     3241 gatccagcaa acgccccgtg cgggaacgac cgccttccac gaagtactcg acggaataac
     3301 cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt ttattgcctt
     3361 taaaagtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg gccggccaga
     3421 agttcaggtt gatcccggcg gcgatatgcg gcggcaccag cccctggtgg tacagcacgt
     3481 aagaaagtag caggtagtcc atgtgactgc ggtggcaagg cacatacacc agctcatgac
     3541 cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgatgccc tgataaagtc
     3601 ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa gagaaattcg
     3661 ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg gagatttttt
     3721 tgctgcgcgc ttcatcttct accgctttgg caatggcgcg ggaggcgagc agcttattaa
     3781 acagatcctg acgagcaggc agacgtgggc ccacagcagc cagacgttga cgggcaaagt
     3841 gcatacgcgc cacgcgcgcc agtttctgag cgatagtttt atctgtgccg tgttcatcgg
     3901 ccatgcggcg cagagaaact gacggcgaga aacgcacaaa actgtcgcga ccgagccaca
     3961 gtacggcgaa aaatttttgt acgccgttaa gcatacgcag cggcgggttt acttcgcctt
     4021 tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc acatccagat
     4081 ttgggttgct gcggtgcaaa tcaagatagt cgtggaacag cttaatagac tcttctttcg
     4141 gcgtgtaata ggtgaacaca cgcggcccgc cgtgaatgaa cacatagcgc ggcagtagcg
     4201 tgccgtcgat ttccagcggc tctaacgggt caggcaagtc atgagccaga cactgggcgc
     4261 gcaacgtcag caaatctgct ttcgagttgt acggtaaaac gtacataatt ggacgagagg
     4321 tatccagccc cagttccggg gcaggatctg ccggaataga cttgcttttt accaggatgc
     4381 ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata aacgatgtaa
     4441 agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg ggaaattctg
     4501 tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga tttaacggta
     4561 cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa taccactgga
     4621 ttcacccgaa ttatcaaagc tgctggctat tcctggaaag gtttacgcgc tgcatggatc
     4681 aacgaagcgg cattccgtca ggaaggcgta gcggtattgt tggcggtggt catcgcctgc
     4741 tggctggatg tggacgcaat tacccgcgtg ctgcttatca gctccgtgat gctggtgatg
     4801 attgtggaaa tcctcaatag cgccatcgaa gcagtggttg accgaattgg ctctgaatac
     4861 catgagcttt ccggacgcgc aaaagatatg ggatccgccg cggtgctgat tgccattatc
     4921 gtcgccgtga ttacctggtg catcctgtta tggtcgcatt ttggataacc cttccagatt
     4981 tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt tgctgtatat
     5041 actcacagca taactgtata tacacccagg gggcggaatg aaagcgttaa cggccaggca
     5101 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg
     5161 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa
     5221 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct
     5281 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccgct
     5341 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttgt tcaagccgaa
     5401 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg
     5461 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg
     5521 tattgatgac gaagttaccg ttaagcgact gaaaaaacag ggcaataaag tcgaactgtt
     5581 gccagaaaat agcgagttta aaccaattgt cgttgacctt cgtcagcaga gcttcaccat
     5641 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata tctctgagac
     5701 cgcgatgccg ccaggcgtcg cggtttgttt ttcatctctc ttcatcaggc ttgtctgcat
     5761 ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca tgattttctc
     5821 caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc atcttgatag
     5881 cccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc tctttatgct
     5941 gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg gtgccaaaaa
     6001 tcctcaggca ttagcccgtg cgctggtgca accgttgctg ttggcgttgg gggctggggc
     6061 gttaattgcg ctgctgcgta caccgattat cgatctggcg ctgcatattg ttggcggtag
     6121 tgaagcggtc ctggaacagg cgcggcgctt tcttgaaatc cgctggttaa gcgctccggc
     6181 gtcgctggcg aatctggtat tactcggttg gttactcggc gtgcaatatg cccgtgcgcc
     6241 agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct ggctggtgat
     6301 ggggctgcat atgaacgtgc agggcgcggc gctggcgacg gttattgcgg aatatgcaac
     6361 attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa tttccggcga
     6421 aatgctgaaa actgcctggc gaggaaactt ccgtcgcttg ctggcgctta accgcgatat
     6481 catgctgcgt tcgctgttgt tgcaactctg tttcggcgcg atcaccgtac ttggtgcgcg
     6541 actggggagt gacattatcg ctgttaacgc ggttctgatg acgctactca cctttaccgc
     6601 ctatgcgctg gatggttttg cctacgcggt tgaagcacat tccggtcagg catacggtgc
     6661 gcgcgacggt agccagttgc tggatgtctg gcgggcagcg tgccgccagt cggggatcgt
     6721 agcgctactg ttttcggtgg tttatttgct ggcaggggaa cacatcattg cgttactgac
     6781 gtcgttaacc cagattcagg agctggccga ccgctatctt atctggcagg tgattttgcc
     6841 gttggttggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa cgcgtgccgc
     6901 cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcgctgacgc tccttacgct
     6961 gccgtggctg ggtaatcatg gtgtgtggct ggcattaacc gtctttctgg cgttgcgcgg
     7021 gctttctctg gcggctatct ggcggcgtca ctggcgcaac ggtacctggt ttgccgcaac
     7081 gtgacggtta aaaattctga ataaataatc ctaagccaaa ttgctgacta cacttaatct
     7141 cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag gtcttgatta
     7201 tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg aaagagcaat
     7261 ggggcaaact gaccgatgat gatatgacga tcattgaagg taaacgtgat caactggtcg
     7321 gtaaaatcca ggaacgttat ggttatcaga aagatcaggc agaaaaagag gtcgtggatt
     7381 gggaaacccg caatgaatat cgctggtaat taatccctcc tgcccgacgt gtacaaggat
     7441 gtacgccctc ttaacgcggt ttctttttca cctgcacaga gtgatcatgc tggcactgtt
     7501 caggatgacg acacgcttcc acttccacgc atgccgcaca gagcccatgt gcttcaatca
     7561 cattatgccg cagggcaaac cccatttttg ccgccagcgt atgcataatg tcttccacgc
     7621 cttctgcaca ctcttctttc accgcgccgc agcgatcgca aataaacatg gctgacgtat
     7681 gcgtgggctg gtcgaacaga tgacagagca cataactgtt ggtggattcc accttatgca
     7741 caaaaccttg ctcaagcaga aaatccagcg cgcgataaac cgttggcggc ttggcttgcg
     7801 gttcagcttc gcgcagcaaa tcaagcagat cataagcgct gatagcgcca tcctgcagac
     7861 tcatcaggcg caacacttcc aggcgctgtg gggtcaggcg cacattacgc tgcgcgcaga
     7921 ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccatcggg cacctcaaag
     7981 tgggggttaa gaatgtcctt actttaccat gttccaggaa aaacaccgag aacccttatt
     8041 gttgccgtaa tgttgatttt ctgttttgta cgtaaggtgt tgtgttgctt ggtcgta
//
LOCUS       DAPHDX010000170         8072 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPHDX010000170 DAPHDX010000000
VERSION     DAPHDX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.172"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            158..2401
                     /gene="pdeF"
                     /locus_tag="RH058_003974"
     CDS             158..2401
                     /gene="pdeF"
                     /locus_tag="RH058_003974"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV3139487.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIIFCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2440..3981)
                     /gene="ppx"
                     /locus_tag="RH058_003975"
     CDS             complement(2440..3981)
                     /gene="ppx"
                     /locus_tag="RH058_003975"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV3139488.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3986..6052)
                     /gene="ppk1"
                     /locus_tag="RH058_003976"
     CDS             complement(3986..6052)
                     /gene="ppk1"
                     /locus_tag="RH058_003976"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV3139489.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6223..6861)
                     /gene="purN"
                     /locus_tag="RH058_003977"
     CDS             complement(6223..6861)
                     /gene="purN"
                     /locus_tag="RH058_003977"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV3139490.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6861..7898)
                     /gene="purM"
                     /locus_tag="RH058_003978"
     CDS             complement(6861..7898)
                     /gene="purM"
                     /locus_tag="RH058_003978"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV3139491.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         1963 a         2013 c         1977 g         2119 t
ORIGIN      
        1 atcagggtta ttttgtttga tatatatcaa ttggaacttt catatgacac ttagaatcaa
       61 tgctctctat agtgatgaat aatcatcatt cgaagtcagg tgggatgcct gtctgaatac
      121 acctccttca ggatgtgggg gattctgctg agcatctatg aaactgaatg caacttatat
      181 aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa tcttgcccat
      241 tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc tcttttatct
      301 gcctctggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat tgccagggat
      361 cgccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa cgctatcatt
      421 aacggctaat tttattatca ttatcatttt ctgttggggc ggttacaggg tctttactcc
      481 ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta tattctggca
      541 gattgtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat ttgtaggatt
      601 actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga ctttaatcaa
      661 ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact tcatcattcg
      721 ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac agcaggttga
      781 tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg ctttattgtt
      841 gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt ataccttgtc
      901 attattgctg cccctgatga tgtggggagc gatgcgctat ggttataagc tgatttcgct
      961 gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca ttcccattta
     1021 tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat tctcttttat
     1081 tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac gcattcagcg
     1141 gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga atcgcgcgtt
     1201 gcgtgatgcc ccctggtctg cgctttgtta tttacgcatc cctggcatgg aaatgctggt
     1261 taagaactat ggcatcatgc tgcggattca atacaagcaa aaactttctc actggctgtc
     1321 acccttgctg gaaccgggtg aagatgttta tcagctttcg ggtaacgatc tcgcgctgcg
     1381 gctgaataca gaatcgcacc aggagcgcat taccgcactg gatagccatc tcaagcaatt
     1441 tcgtttcttt tgggatggca tgccgatgca accgcagatt ggcgtcagtt actgctatgt
     1501 gcgctcgcca gtgaatcata tctacctgct gctgggagag ctaaatacgg tcgccgaact
     1561 ttccatcgtg accaacgccc cggaaaatat gcagcgtcgc ggggcaatgt atttgcaacg
     1621 cgaattgaaa gataaagtcg cgatgatgaa tcgactacag caggcgctgg aacacaacca
     1681 ttttttcctg atggcccagc cgattaccgg tatgcgtggt gatgtttacc atgaaattct
     1741 tctgcgcatg aaaggtgaga atgatgaact gatcggcccc gatagcttct tgccggtcgc
     1801 gcacgaattt ggtttatcgt cgagtatcga catgtgggtc attgagcata cgctgcaatt
     1861 tatggctgaa aacagagcga agatgcccgc tcaccgtttt gctattaatc tgtctccaac
     1921 ctcggtatgt caggctcgtt ttcctgttga agtcagtcag ttgctggcta aatatcagat
     1981 tgaagcgtgg caacttattt ttgaagtcac cgaaagtaat gctctgacca atgttaagca
     2041 ggcgcaaatc accttgcagc atcttcagga attaggctgc cagattgcga ttgatgattt
     2101 cggcaccggc tacgccagct atgcacggct taaaaatgtg aatgccgatc tgcttaaaat
     2161 tgacggcagt tttatccgca atattgtgtc aaatagtctg gattatcaga tagtggcatc
     2221 gatttgccac ctggcgcgaa tgaagaaaat gcgggtagtg gcagagtacg ttgaaaacga
     2281 agagatccgc gaggcggtgc tctctttggg gatcgattat atgcagggtt atcttattgg
     2341 taagccgcag ccgttaattg atacgttgaa tgaaatcgaa cccattcgcg aaagtgcctg
     2401 aataatgcgg gccgacattt ctcgtcggcc cgcaaagcat taagcggcga tttcaggtgt
     2461 actttcttct tcaattttca accgccagcc agccacgcct tcccagtatt cttgctcctt
     2521 ttccagatca agcagtacca gcgcattctg actaaaccag tcatgcggga aacgcagtgt
     2581 ccagtgactg tcatcagtaa tcagtgtcaa tgttggcggt gtggtggttg cctgacgttg
     2641 attgtttagt aatacgccaa ggcgcaatag ctgtatcagt ggcaggaact gtttcttctt
     2701 aaacaaggta aagcgcggca gatcgtcgag cttaatcgct ttacggtgat agcgcaccag
     2761 tgtcgccatc atcagctgct gttcctgatt aaaacccggc aagtcactgt tttgcagaat
     2821 ataagcggag tggcgatgca aaccgctgtg gttgatattc aacccgacct catgcagcat
     2881 ggcggcccat cgcagtagcg cctccagttg cggatgcgcc agcttcggtt gctgttcccg
     2941 ccactgttcg tacatttgca tagtggtatc cagcactcgt cgggcctgtt cgctgtcgat
     3001 gtgatactgg ttggcgaggc tgctggcggt gcgactacgc acatcctgat gacggaaacg
     3061 tccttccatt tcatacagta cgccttcgcg taacgccccg tcagaaaggc gcagttcacg
     3121 gatggctaaa gcatcaaaca caccgcataa aatcgccagt ccaggaacga agactgtttt
     3181 ccgctcttcg gaaagacccg gtaaactcag cgatgcgaaa ttacggtgac gtaaaacttc
     3241 ttttaccagt ttttccagac gttccggggt aattatcccg tctttctcgc ccatttccat
     3301 taacacttca tgggcggctt ttatggtacc ggaagcgccc attgccacgt tccagccctg
     3361 aatacggaat tgccaggtta aagtttccag tttttgtgct gccgccatac gagcgcgctg
     3421 aaaattctct ttattgatga ccccgccagg gaagtaaagc tgggcaaagc tgacacaacc
     3481 catccggcgg ctttcaacga gaatcggttc gaaattttca ccaatcacca gttccgtaga
     3541 tccgccgcca atatcaataa ccagtttgcg acctttctcc ggttgggtat gttccacgcc
     3601 cataaaaatc agacgggctt cttcattacc ggaaataatt tcaatcgggt agggaatgac
     3661 cttttccgcg cgtttcagaa agtcagtggc gttcagcgcc tgacgcagcg tatgggtgcc
     3721 gactatacag acgctggcag gagaaaaccc ttgtagccgt tcggcaaaca gcgacagaca
     3781 gttcaaaccg cgcgtcattg cctcttcact caacatatta tctggcccca ggccgtccgc
     3841 cagatgcacc cgctgtttca ggcggccaat aatctgcatg gcaccatcta ccacacgggc
     3901 tatgaccatg tgaaaactgt ttgaaccaag atcgaccgca gcaaactcct gcggacgagg
     3961 ggatttatcg tgtattggca tagggttatt caggttgttc gagtgatttg atgtagtcgt
     4021 aaatcgccaa ctgcgcccgt actttgcggc gattgccgcg gggaacgtag cgattactga
     4081 gttctttatc gatataacgt gctttgaccg tatcgctgaa caatatgtcg atgatgtcca
     4141 gcacccgctg cttcaggcgc ggatcgagca gcggcgtcgc cacttcaata cgataatcaa
     4201 tattgcgcgt catccagtcg gcggaagaaa ggtagacctt tttatcgccg ccattttcaa
     4261 aaatataaac ccggtcatgt tcaaggtaac ggtcaacaat actgatggca cgaatgttgt
     4321 cgctaatgcc ttccagattg gggatcagcg aacacattcc gcgaaccagc agattaaccg
     4381 gtacgccgga gctggaggcc gcatacagac gatcaaccag gcctttatcg acaaggttat
     4441 ttagcttcag ggtgataccg ctgggcagcc cttgctgcgc gttggcgatt tcgcggtcta
     4501 ccatttcata taacagacgg cgagagtttt gcggtgacac cattaaataa tcaaatgtca
     4561 ccgggcggta tgggttttca ataaagttaa atacccgccg tacttcgttg gtgatacgcg
     4621 catcggcggt cagcaatgaa tagtcagtat aaagacgcgc ggttttttcg ttaaagttcc
     4681 cggtcccgat gtgtgcgtaa cgcaccactt caccgttttc ttttcgtgaa atcaggaaca
     4741 gtttggcgtg aattttcagc cccggcgcag agaagataac gtgcacgcct gcttcggtca
     4801 ggcgcttcgc ccagtgaatg ttagcttctt cgtcgaaacg cgcctgtaac tcaaccacca
     4861 cggtgacttt cttaccgtta tgtgcggcgt ggatcatcga gtcgatgatg cgtgaatctt
     4921 tcgccacgcg gtaaatgtta attttaatcg ccagtacgct cgggtcgaac gaagcctgac
     4981 gcagcagttc cagcacatgc tcaaaggtgt gataaggata atagagcaac acatcgcgtt
     5041 cgcgaatggc atcaaaacca ttgcggaact gggctttatc aaaccaaata tggcgtaaac
     5101 gcggcagtgg tttgttcacc agattggctt tgccgacatt ggggaaatta ataaagtctt
     5161 taaaattatg ataacgaccg ccggggacga tggagtcgta gcgggaaata gtcagttttt
     5221 cgcgtaacac ttcaaccagc gcattgggca tatcgcgctg ataaacaaaa cgcaccggct
     5281 cagcagttaa acgctgcttg agactggaag acatcaactc catcaggctg gcttccatct
     5341 catgcactaa atcgtattcg gcatcgcggg tcatcttcat tgaataggca ttcagcgcgt
     5401 cataatcaaa gaagcctttg aaaatatcat caaggcagta acgcagaatg ttatccagaa
     5461 gaatcatcgg cttgcgtcga cgcggcgctt ctggcggtaa attcacaaag cgcggcactt
     5521 tatctgatgg gatctccagc agcgcgtaac ggatggtatc gccacggata atttccaccg
     5581 ccagataggt gtaatcatct ttcaggaact gcactaagtc agtgtcagga ttgattaaaa
     5641 tcggcgtaat gtgctgacgc agatactgct taaaataatg acgcagccag ttttgttgat
     5701 tgacggagag ctggcgttca ttaatcagga agatctggtt acgcgccatc tccagcagca
     5761 gctcgttgta gaggccgtcg aattcctgat cggctttcag cacccgggac tgaattttgc
     5821 ccagtaaatg gcgggaatga gagttggagc cttgttcttc gctaataatg atgcgtcgct
     5881 tcagttcagc gaagcggact ttatagaact catcaaggtt attggaatag atccccagga
     5941 aacgcatcct ttcaatcagc gggttagatt tgtccgccgc ttcctgaagc acgcgttcat
     6001 tgaacgataa ccaactgagc tctttttcga tgtatagctt ttcctgaccc attaccactt
     6061 ttactccgtt ttattcacgg gacactgcct ggatattatg gcgagcaata ttggcagatg
     6121 tccaactatg ccagaaaagg gggaaaaaaa ctgctggagc ttgcgattta caggtgaatt
     6181 gaaaaacgcc agcggcagag ctggcgcttt aactacgggg cattactcgt cggcagcgta
     6241 gccctgcggc ggcagacgtt gaccatccag ccacgcggcg ttttcgtgca ttttcagacg
     6301 accatcggca aaccagctaa tcaccagtgg ataaatagcg tgttcctggg tttgcacgcg
     6361 ggcggtgatg tcatcttccg tatcaccagc aaataccggg actttcgcct gtaaaataac
     6421 cgggccaccg tccagttcat cggtgacgaa atgcaccgat gtaccgtgct cttcatcacc
     6481 attttccagc gcctgacgat gggtgtgtaa tccgggatat ttcggcagca gagaagggtg
     6541 aatgttcagc aaacgcccgg catagtggga gacaaacgcc gggctgagaa tgcgcataaa
     6601 accagccagc acgaccacat cgggtgcgta catgtcgatt tcatgaatca actcccggtc
     6661 ataggcctca cgactgtcaa acgcgctggc gatgagcgta tgcgttgcaa tacccgcctg
     6721 gcgggcgcgt tcaaggccga acgcgtcggc cttattgctg aaaactgccc gtacggtgcc
     6781 tttaattttg ttggttttac aggcgtcaat aattgcctgt aaattacttc cgttgccgga
     6841 aataagcacc acaatattca ttattcgata accacgcgtt gttcggaatc agaggctttg
     6901 atgataccga ttttccacgc gttttcaccg ttggcattga gtagggcgag ggctttgtcc
     6961 acttccggag caggcagggc aataatcatc ccgacgccgc agttgaaggt gcgatacatt
     7021 tcatggcgct caacgttacc tgccgtttgc agccagttga acacttccgg ccactgccag
     7081 gaagattcat caatcactgc ctgggtatta tctggcaata cgcgcggaat gttttcccag
     7141 aagccgccgc cggtcaggtg cgcaatggca tgcacatcga ccttttcaat caactccagc
     7201 actgacttca cgtaaatgcg ggtcggtgcc agcagatgat cggctaatgg cttaccatca
     7261 agttcggtgg tttgcggatc acaaccgctg acttcaagaa ttttgcgcac cagcgaatag
     7321 ccgttcgagt gcggaccgct ggaaccgagt gcaatcagca catcgccgtc gctgacttta
     7381 gagccgtcga tgatttctga tttttctacc acgccaacgc agaaacccgc gacatcgtaa
     7441 tcttcaccgt gatacatccc cggcatttct gccgtttcgc cacccaccag tgaacagccc
     7501 gattgcagac atccttccgc aatgccgctg atcaccgctg aagcggtatc aacatccagt
     7561 tttccggttg cgtaatagtc gaggaaaaac agcggttctg caccttgcac caccaggtca
     7621 ttaacgcaca tggcgaccag atcaatacca atggtgtcgt gacgttttaa gtccattgcc
     7681 agacgcagct tggtacctac gccgtcagtg ccggaaacca gcacgggttc acgatatttt
     7741 tgcggcaatg cacacagcgc accgaagccg cccagaccgc ccatcacttc cggacgacgc
     7801 gttttcttca ctacgccttt gattcttcca accagagcat tacccgcgtc aatatcaaca
     7861 ccggcatctt tgtagctaag agaggtttta tcggtcactg cttgggtccc cacgcgttac
     7921 ttgcggtaga aaaataaaat tcggcgcaat tctaacaggg aaagcaaacg tttgcgagac
     7981 tgctttacac aacctttttg cacgtctttt ccccagacgc gcggcgaaag aagacttgtg
     8041 ccagggtaaa ggttagtttt cggatggaat aa
//
LOCUS       DAPHDX010000171         8009 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPHDX010000171 DAPHDX010000000
VERSION     DAPHDX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.173"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..1459
                     /locus_tag="RH058_003979"
     CDS             116..1459
                     /locus_tag="RH058_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3139492.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1684..3339
                     /gene="opgD"
                     /locus_tag="RH058_003980"
                     /gene_synonym="mdoD"
     CDS             1684..3339
                     /gene="opgD"
                     /locus_tag="RH058_003980"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV3139493.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3479..3703
                     /locus_tag="RH058_003981"
     CDS             3479..3703
                     /locus_tag="RH058_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV3139494.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3766..4302
                     /gene="rimL"
                     /locus_tag="RH058_003982"
     CDS             3766..4302
                     /gene="rimL"
                     /locus_tag="RH058_003982"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV3139495.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4297..5277)
                     /locus_tag="RH058_003983"
     CDS             complement(4297..5277)
                     /locus_tag="RH058_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV3139496.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5401..6393
                     /gene="tehA"
                     /locus_tag="RH058_003984"
     CDS             5401..6393
                     /gene="tehA"
                     /locus_tag="RH058_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV3139497.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6390..6983
                     /gene="tehB"
                     /locus_tag="RH058_003985"
     CDS             6390..6983
                     /gene="tehB"
                     /locus_tag="RH058_003985"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV3139498.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7286..7954
                     /locus_tag="RH058_003986"
     CDS             7286..7954
                     /locus_tag="RH058_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV3139499.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         2037 a         1875 c         1994 g         2103 t
ORIGIN      
        1 agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa ttcttcccgc
       61 cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag acactatggc
      121 gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt cagccatgta
      181 ccagcaagaa gttccgcaat atggcacgct gctggaactg gtagctgatg tgaatctggc
      241 tgtgctggaa aacaatcctc aactgcacga aaaaatggta aacgcagacg agctggcgcg
      301 actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc ttgctactct
      361 tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc tctcgcaggc
      421 aggggtgccg gtacattcga cagcatttcg gcccattgat gatgcttctc tggcgcgtaa
      481 tcccttccgc gtttttacct ccttactccg ccttgagctt atcgagaacg aaattttgcg
      541 ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt gtcgacaact
      601 gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg agtttgtgca
      661 ggaagccctg gaaacgtttc gctggcacca gtcagcaacg gtagatgaag aaacctatcg
      721 cgccttgcac aacgaacatc ggttgattgc tgatgtggtc tgttttcctg gatgccatat
      781 caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga tgcctgaatg
      841 cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac cgattttact
      901 acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc agaaacaggg
      961 cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa cgccgaaagg
     1021 gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata atctcactca
     1081 ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt taatgcgtca
     1141 gcaagggctg gcatggttcc ggtaccgtct gacgccttca ggtgaggcgc atcgtcaggc
     1201 gattcatccc ggagacgatc cacagccctt aattgaacgt ggttgggtcg cggcgcaacc
     1261 cattacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt caaatctggg
     1321 taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg agcaggcgtt
     1381 gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac gcagtaaacg
     1441 tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc ctgattttac
     1501 aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca tattctctca
     1561 gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa ataagcactt
     1621 tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga ctcactttca
     1681 ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt gtgcggtacc
     1741 agcggcattg cttctctttt ttctcaggcg gcattcgcgg cagattctga tattgccgac
     1801 gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga cttagcgcaa
     1861 acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat gacgccgcag
     1921 gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt tgagaaccgt
     1981 caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt tcgtatgttt
     2041 tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa
     2101 tacaacgatg cgggtgttga tacaaaacaa ttagaagggc aaagcgatct cggctttgcc
     2161 ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc atttctcggc
     2221 gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc ccgcggcctg
     2281 gcgatcgaca cttacactga cagtaaagaa gagttccccg actttaccgc cttctggttt
     2341 gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga tagcgccagc
     2401 attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat tatggatgtg
     2461 gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc gatgaccagt
     2521 atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc gcaaattcat
     2581 gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg tccgctgaat
     2641 aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg gtttggttta
     2701 ttgcaactgg atcgtgattt ctcccattat caggacatta tgggctggta taacaaacgc
     2761 ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa
     2821 atcccaacaa cgggcgaaac gctggataac attgtctgct tctggcagcc agaaaaagct
     2881 gtaaaggcgg gtgatgagtt tgcattccag tatcgtctgt actggagtgc gcaaccgcct
     2941 gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa
     3001 ggttgggcac caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc cgtcgatttc
     3061 gttggtggtg atctgaaagc tgccgcacca aaaggcattg agccggtgat tacgctttcc
     3121 agtggggaag cgaagcaaat cgaaattctc tatattgaac ccattgatgg ttatcgtatt
     3181 cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg gatgtatctg
     3241 cgttgtcagg gcgacgctat cagtgaaaca tggctgtatc agtatttccc gccagcgccg
     3301 gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct tcggcacctt
     3361 cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct aagttaaatg
     3421 agtaatctga tggtgtgtat ttcagataca ccttgtcagc cactaacagg gagtgcgtat
     3481 gtttccagaa tatcgagatt taatatcccg tctgaaaaac gaaaatcctc gctttatgtc
     3541 cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag gttccgacgg
     3601 tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt taaaagatga
     3661 gatgctcaaa atcctgcagc aggagagcgt caaagaggta taaactttcc taagccgcca
     3721 gccaggcggc ttttttaaca actgcatgga ttgactggag ataagatgac tgaaacgata
     3781 aaagtaagcg aatcacttga attacatgct gttgcagaaa atcacgtcac tcctctttat
     3841 cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc gcagttcgtt
     3901 cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc
     3961 tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat ctcgtttaat
     4021 cgtattgaac cactgaataa aaccgctgaa ataggctact ggctggacga atctcatcag
     4081 gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc ccagtctggt
     4141 gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa ccaggtcgct
     4201 ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct gaatgatgcc
     4261 tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg ccagcggcgt
     4321 tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa ccgtgtcgcc
     4381 atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa ttatcaccgc
     4441 acctgtaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa tcggtccacc
     4501 acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat taccttccac
     4561 aatggcatat tccgccacct gcgaactgta atgaatcgtt ggaatggcat cttcttctat
     4621 gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc agacgttgtt
     4681 ctcttcatta ccttcaatac tggcaaaatc aaaaacttcg gcacgatgtt caataaaagc
     4741 ataccggatg acggcatcgc cataaatttg tgcctggtgg acaatacgcg aggcgctaac
     4801 ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc cttgtgcggc
     4861 gacgatcata gaatgttgat taattaaggc gtgaccaaat attctacatt gcccgcaaac
     4921 cagtgagtca tgaatggtga cgctgtcact tatatatgca ccctggctga tttcactatt
     4981 atcgatccag acattatccg ttgcgtaaac ctctccccac agcacgctgg tgccggtgat
     5041 gcgcgtatta ccggaaatca ccgctccgcc gaacgcaata gcgttctggt cgtaaatcca
     5101 gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc cagccgccag cggttcctgc
     5161 tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacat tttttttagt
     5221 gccatcttcc tgataactaa aggctcgctg ttcttcactt agacgatatt tgcgcatagc
     5281 gttttcccac aggtgactta ctataaccgt agcaaattct gcggctctgg ctatgctcga
     5341 gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat gagtaagaag
     5401 atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt gttggggacg
     5461 atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag ccactggtta
     5521 ggagatgggc tggtgattct ggcgatgatc atctggggat tgttaactag cgcatttatt
     5581 acccgactta tacgctttcc gcatagcgtg ctggcagaag tgcgccatcc agtgctgagc
     5641 agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt tgttccgtgg
     5701 tttcgcccac tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt ggcttatgcc
     5761 gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac cacgcctgga
     5821 ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg tgcgttgggc
     5881 tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct aagccttgaa
     5941 cctgtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact gcggacatca
     6001 ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg cctggttgag cgtcaacggc
     6061 ggcgagggtg atacgctggc gaaaatgctt tttggttatg gactgctgca actgctgttt
     6121 atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt ctggagtttc
     6181 tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg cagcgataat
     6241 ggatttttcc atacgctggc ggtgccgctg tttatcttta ccaattttat tattgcaata
     6301 ctgctcatcc gtactttcgc gcttctgatg cagggaaaat tgttagtcag aaccgagcgc
     6361 gccgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac tattttactg
     6421 ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa gtggttaaac
     6481 cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac ctggcagcca
     6541 atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaac gtcgagcgca
     6601 ttaaatccat tgaaaatctg gataatttac acacccgggt cgttgatctg aataacctca
     6661 catttgatgg acagtacgat tttattcttt cgactgtggt gctgatgttc cttgaggcta
     6721 aaaccatccc cgggttgatt gccaatatgc aacgttgcac taaacctggt ggttacaacc
     6781 tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc ccgtttgcct
     6841 tcaaagaggg agaattacgt cgatattacg aaggctggga gagggtgaaa tacaatgaag
     6901 acgtcggcga gctgcaccgc accgacgcca acggtaatcg tattaaactg cgtttcgcca
     6961 cgatgctggc acgtaaaaaa tgacccgcta agaataaggc gcgtgaaaat tccccacgct
     7021 gagatgattt actgttcttc ttttcggtaa gcatattttt tatcgaaggg atgtgaaatt
     7081 aatcacagta gtcgaagttt tttagcagct taacttactg aaatttaact actgatgatt
     7141 gacttagccc ctttttcggc attgactatg tcgtctgaaa aggggctgaa aaatttattt
     7201 tcaccaacac tttttttgcc acaacacgaa gcggcgcttt ttgctataac ttaaaaagta
     7261 atataatcat ctcaggaaac tattcatgcg taccacatca tttgcgaaag ttgcagcttt
     7321 atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc acccagccag ataaatattc
     7381 tggtttttta aacaattact ctgatttaaa agaaacaacc tcagcaacag gtaaacctgt
     7441 tttacgttgg gtagacccga gttttgatca aagcaaatat gacagcatcg tctggaaccc
     7501 aatcacttat tatccggtgc caaaaccttc tacccaggta gggcagaaag ttctggataa
     7561 aattttgaac tataccaaca ccgaaatgaa agaagcgata gcgcagcgta aaccagtggt
     7621 taccaccgct gggccgcgta gtctgatttt ccgtggggcc attaccggtg tagataccag
     7681 caaagaaggg ctgcaattct atgaagtggt tcctgttgca ttagtggttg cggggacgca
     7741 aatggctaca ggccaccgta ccatggatac tcgcctctat tttgaaggtg agctgattga
     7801 tgcagcgact aataaaccgg ttatcaaagt cgttcgtcag ggcgaaggta aagacctgaa
     7861 taacgaaagt acgccaatgg ctttcgaaaa tattaaacaa gttattgatg acatggcgac
     7921 cgatgccacc atgtttgacg ttaacaaaaa gtagttccag acgcgccatc gttagatggc
     7981 gctttttatc cggtgcgccg taaaacccc
//
LOCUS       DAPHDX010000172         7766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPHDX010000172 DAPHDX010000000
VERSION     DAPHDX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.174"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            184..402
                     /locus_tag="RH058_003987"
     CDS             184..402
                     /locus_tag="RH058_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV3139500.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            621..863
                     /locus_tag="RH058_003988"
     CDS             621..863
                     /locus_tag="RH058_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139501.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1193..2122)
                     /gene="fdhE"
                     /locus_tag="RH058_003989"
     CDS             complement(1193..2122)
                     /gene="fdhE"
                     /locus_tag="RH058_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV3139502.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2119..2754)
                     /gene="fdoI"
                     /locus_tag="RH058_003990"
     CDS             complement(2119..2754)
                     /gene="fdoI"
                     /locus_tag="RH058_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV3139503.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2751..3653)
                     /gene="fdoH"
                     /locus_tag="RH058_003991"
     CDS             complement(2751..3653)
                     /gene="fdoH"
                     /locus_tag="RH058_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV3139504.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3666..6716)
                     /gene="fdnG"
                     /locus_tag="RH058_003992"
     CDS             complement(3666..6716)
                     /gene="fdnG"
                     /locus_tag="RH058_003992"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6129..6131),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV3139505.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6910..7743
                     /gene="fdhD"
                     /locus_tag="RH058_003993"
     CDS             6910..7743
                     /gene="fdhD"
                     /locus_tag="RH058_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV3139506.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1802 a         2006 c         2066 g         1892 t
ORIGIN      
        1 attcatcctg ggcgataact aaaccatttt tgagtaataa atgaaacaac tgcaacactc
       61 ttattatttc tggaaaacgc ctcgcaaaaa caaaacaaca tgcaaaaatc cgcttttttt
      121 aaatgatttt ccggcgtaat tttcgcacat acattaatga gtaatatagg taaggtggtg
      181 gtaatggcga tgaatacggt ttttcttcat ttatcagaag aggcaattaa acggctgaac
      241 aaacttcggg gctggcgtaa ggtttcacga tctgcaattt tacgcgaagc ggttgagcaa
      301 tatctggagc ggcagcaatt tccggtgcgt aaagcaaagg gaggcaggca aaagggggag
      361 gtagttggtg ttgatgatca gtgtaaggag cataaggaat gaaaaaaatt tttcattctt
      421 gaatataaaa aaacagatgc ccttattctg gtattaatac aaggctgttt tacttgaact
      481 tataataact gcaactgtta catcatatct ggaaaacgcc tcgcaaaaat aaaaaatgat
      541 tcgtaaatga gctttcttta aagattcgac aagggtactt ttctctccat agattcaaaa
      601 atgatattgg cgagatattt atgaactctc tggtaggtat agatatgggc agaattttac
      661 tcgatttatc gaatgaggtg attaagcaac ttgatgatct cgaggtgcag cgtaatcttc
      721 ctcgtgcgga cctattaagg gaagcggtag atcaatacct gataaatcaa tcgcaaacag
      781 caagaaccag tgttcctggc atctggcaag ggtgtgagga agatggtgtc gaatatcagc
      841 gtaagctgcg cgaggaatgg taatgggaaa aaatgatgtc aatcagattg ctgacaacgt
      901 gcgcgttgtt catgccggat gcggtgtgaa cgccttatcc ggcctacaaa gtcatgcaaa
      961 ttcaatatat tgaagtttat tggtagggct gataagcgca gcgcatcagg caatcatacg
     1021 cttgtcatca gtgacatacc cataacctta tcgatgtgtt ggagggaaga acgaggcaaa
     1081 cagacaattg cggcatgatc ttgaatgggt aacgcggaat acgagagatg gtgtcgtcac
     1141 accctatgag ctgtaatccg ggcgaccagc gccacccgga aaacgtcaga gattactccc
     1201 cttcacccgg aaacaggaac gggttgatgg aactgcgggc atagccttct tgctccattc
     1261 gcgcgtccag taccagagaa gcgaggtcat ctgccacggc ttcaactttc ggttcttttt
     1321 cctgataaag aatcttcagg taagtgccac agtcatcgca gctttcggct ttaatcgcgg
     1381 cctgttcgtc atccagcgac cagtaatgca gtttgccgct ctgctcgcag ttgctgcatt
     1441 ttacgcgcac tacgtgccat tcggtttcac acaggttgca gtgcaggtaa cgcagaccct
     1501 gagtggtgcc aatttgcacc atgctggaca ccggcataga accacaaacc gggcaatatt
     1561 gacgttgctc gccgtattca gcgcgggctt tgccggggat cagattggcc atctgcgccc
     1621 agtagagcga cagtgcagcc cagataaacg gcgctttatc gctgctgacg gatgagaaat
     1681 cagaggcaaa cagtgcgctg gccatatctt ccagctcctg agtcgatgcc ttctccagat
     1741 tctcaatcac tgccagcgcc gggccgctca tttcaggttt cagctcagca atcagcgcca
     1801 tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg aatatccagc gggggcttgc
     1861 cctgtgcgct ggcttcttta atgcgcgcgg tcagatccat ctccagcgga tggtcgtaca
     1921 gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg caggtaatca cccagcggat
     1981 tattttctgc cagctcgcgc agacgctcgg cgcggcggtt gtataaattc ttgagccgag
     2041 ggaacaataa cggcggaatc atatccgccg tacgtttctc gctcgaaccc agctcatctt
     2101 gcgggattat gcgaatactc attcagcttt cttttccgtt gtcttgcgga cctcacggta
     2161 ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc catccttcca ccatggcggt
     2221 aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc atgataacca caattaacgc
     2281 cactgcggca aatgaatgca gcattaacgc gaatcggatc accgggattg agaaagcagg
     2341 cgcaaaataa ggacgccaga taatcacgcc gctcaccagc agcagaacca ggaaaataat
     2401 cgccgcccag aaaacgcatt tctgaccgaa gttataacgc ccggtgtcac ctacttcctc
     2461 gttgacgacg atcttacgaa tattcttcgc ccaaaagata tcatcccgat tgattaggtt
     2521 gtgatgccag taacggaaaa acatgatgat gaacgaggca aacataacca cgccgacaaa
     2581 cgggtgcaga attcgcgcca gctgcggtgt gcccatgatt tgcatcaacc agttgaagga
     2641 cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg aagcagaagg cggtgatcca
     2701 gtggttgata cgttccggcg cggtgtagcg cacgatggtg tcacgtcgtt tcatttgcgc
     2761 tcctcgtctt tctcttcgtg cagattattc tcttcctcat ccgcacggtt cggaccgaca
     2821 cccacgtagt ggaagatact ggccgcaaag gtagccgcaa agccaacagc cgcgagcggt
     2881 ttccagatgc ctttccagaa tttcacggtt tcgctgattt ccgggttctc cggcaagcca
     2941 tgatacagat ttggcttgtc agcatggtgc agcacgtaca tgacgtgtgt accaccgacg
     3001 cctgccggat cgtacagacc cgcattgtcg taaccgcggg ttttcagctc agcaacgcgc
     3061 tcgctcgcca gcgttttcat cgactctttc gtaccgaagt gaatcgcgcc cgttgggcag
     3121 gtcttcacgc aggctggttc ttgcccaacc accacgcggt caacgcacag cgtacatttg
     3181 tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga acggacagcc cgcaatgcaa
     3241 taaccgcagc caatgcactg ctcggactgg aagtcgacga taccgttggc atactgaata
     3301 attgcacctt ccgccgggca cgctttcagg cagcctggat cggaacagtg catacagccg
     3361 tctttgcgga tcagccattc cagtttgtcg ttctgctcca cttccgagaa gcgcattacc
     3421 gtccacgatt tggcgcttaa atcattgggg ttgtcgtaca ccccaatgtt attgccgacg
     3481 gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac aggctttaca gccgatacag
     3541 gtggtgacgt cgatgagttt cgccacttct tcctggaagt cccgcgcctg aggcgcgggg
     3601 gtcagaccgt tagtcgcgga acgacgaatg atatcttgag attgataagc catatgtcgt
     3661 ctccgttaca ccttttccac attcacaagg aaggacttaa actccggcgt ctgcgtgttc
     3721 gcatcaccaa cgaatggcgt taacgtattg gcaataaagc cttttttagc tacgccttca
     3781 taaccccagt gaatcggaat accgatggta tcgatatctt tgccgttcgc tttcagcgta
     3841 cgaatacgtt tggtcaccac cgctttggct ttgatatagc cacggttgga ggagactttc
     3901 acggtatcgc cctgggcaat gccaagttta ttcgccagcg actccccgat ttccacaaac
     3961 tgctctggtt gcaaaatcgc gttcaacagc gcgtgtttgg tccagtagtg gaagtgctcg
     4021 gtcagacgat aggtggttcc gacatacggg aacttatcgg ctttacccaa tgcttcggcg
     4081 tcgtctttaa agatacgtgc agccgggttc gagataacgt ttggatgcag cgggttagtt
     4141 cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg gaccttccgc catcttatcg
     4201 agggcaaaca gacgccccat gccttcctgc tgcatgataa acggcccgac gccgctaccc
     4261 ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc acttagtgcc gtcccatttc
     4321 agcaactgac gcttcggatc ccacgggtta ccctgcggat ctgcggaggc gcggttatac
     4381 agaatgcggc ggttaagcgg ccatgcccat gcccagccca gcgtgttacc gaggccagac
     4441 ggatcggcgt tatcacgacg cgccatctgg ttgccttccg gcgtccagct accggcgaaa
     4501 atccagcagc cacaggaggt tgtaccgtca tcgcgcaatt gggcgaacga gctaagttgt
     4561 tggcctttct tgacgataac cgcaccggtt gccggatcgg taatatcggc cagcgcctta
     4621 ccgttgctct ccatcgctac ttcttccgac ttcggctcat gcggaatggc gtagttccag
     4681 gtcatgttca gcacctggtc cgggttcgcg ccaccctgtt cggcatacat cttgcgcaag
     4741 cgcaggaaga taccggagag gatctcgcca tcagtcagcg caatccccgg ggcgtccgca
     4801 cctttccagt gccactgcaa ccagcgacct gagttaacga ttgaaccgtt ctcttctgcg
     4861 aagcaggtcg atggcagacg gaacacttcg gtctggatct tcgacgagtc aacttcgttc
     4921 agctcaccgt ggttctgcca gaagttagag gtttcagtgt tcagtgggtc gatggtgacg
     4981 aggaacttca gtttcgacag acaaccgatc actttgtttt tgttcgggaa tgaggcaaca
     5041 gggttaaagc cctggcagat atagccattg accttgccct ctttcatcat ctcgaagtac
     5101 tgcaggacgt cgtagccttt atcccacttc ggcaaccagt caaagcccca gctattttcc
     5161 gccgtcgctt tatcaccaaa gaaggccttc atcatagaga cgaagaattt cgggtagttg
     5221 ccccagtagt ttacctggcc ttccagcagc ggttttggcg tgttggcggt aaggtaggtt
     5281 tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac ctggcagact ctgcgacagt
     5341 agccccaggt ccgtcagccc ctgaatattg gagtgaccgc gcagggcgtt aacgccgccg
     5401 ccggccatcc ccatattgcc gagcagcagc tggatcatcg ccatcgtacg aatgttttgc
     5461 gcaccaacgg agtgttgcgt ccagccgagg gcatacagga acgaggcagt tttatcgtga
     5521 gcactggttt ctgcgatgta ttcgcagact ttcaggaacg cgtcttttgg cgtaccacag
     5581 atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct gtttcagcaa gttccacacg
     5641 cagcgcggat gttgcagcgt ggtatcgcgt ttggcgaagc cgttttcgtc cagttcataa
     5701 gtccaggagg atttatcgta cttgcgtttt tccgcgtcgt agccggtgaa caggccatct
     5761 tcaaagccgt aatcctcacg cactatcagg ctggcgttgg tataggcttc ggtgtattcg
     5821 cggttgaatt tttcattgtt cagcaggtac agcaatacgc ctgacaggaa agcaatgtca
     5881 gtaccggaac gaataggggc atagtagtca gctaccgccg ccgtacgcgt aaagcgagga
     5941 tcgatcacaa tcagcttcgc gccgttgtga attttggctt ccatcgccca gcggaacccg
     6001 accgggtgag cttcagcggc gttaccgccc atcaccacga cgaggttggc gttcttgatg
     6061 tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt
     6121 ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca tacccagcgc gcgggagaat
     6181 ttttgcgtta aatagccggt ttcgttactc gacgcggaag cacacagcat cccggtggag
     6241 agccagcggt taacagtcac gccttcggcg ttttgcgcaa tgtagttagc atcgcggtct
     6301 tctttcatca gtttggcgat gcgatcaaac gcctcttccc aactgatttg ctgccattta
     6361 tcagaacctg gcgcacggta ttccggaaac ttcagacggc tttcagagtg gatgaaatcc
     6421 accaggccag cgcctttcgg acaaagtgca ccgcggttga ccgggtgatc cggatcgcct
     6481 tcgatatgga agatagatgc tttggcgttt tttgctccgt caccgaggct gtacatcaac
     6541 agcccacagc ctacggaaca ataggtgcag gtattacggg tttcgcgggt gcgcagcagt
     6601 ttatactgcc gggtttccgc gagcgctacg ctgggtgcaa aacccagtgc cgctgccgtg
     6661 gtgcctgcca taccgccagc gcagatctta aagaactgcc ttctgctgac ctgcatggat
     6721 tgctccttgt ttcgacattg tcacgtccca tttacattcg cttgctgcgt gtgcagggag
     6781 tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc agaattggct caatttccct
     6841 ccatccagga agggtttgta acagaatacc ataatgttgg tgtgtgtgtt cttatctggt
     6901 taagagaaag tgaaaaaaac acagcaaaaa gaaatcgaaa atgtgacaaa tatcacaggt
     6961 gttcgtcaaa ttgagttatg gcggcgtgat gatttacaac acccacggtt agatgaggtc
     7021 gcggaagaag ttcccgttgc gctggtctac aacggcattt cgcatgtggt gatgatggcg
     7081 tcgcccaaag accttgagta ctttgcgctc ggtttttcgc tttccgaggg gataatagaa
     7141 agtccgcgcg atatcttcgg catggatgtc gttccttcct gtaatggtct tgaagtacaa
     7201 attgagcttt ccagccgccg ctttatgggg ttgaaggagc gccgccgggc gctggcggga
     7261 cgtacgggct gtggcgtatg cggcgtggag caacttaatg acatcggaaa accggtgcag
     7321 ccgctaccgt tcacccagac gtttgatctc aacaaactgg atgatgcatt acgtcatctc
     7381 aacgattttc agccagtggg gcaactgact ggttgtactc acgccgctgc ctggatgttg
     7441 ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc gccatgtggc gctggacaaa
     7501 ctgttaggcc gccggtcaca agaaggggaa agctggcagc aaggtgcggt actggtttcc
     7561 agtcgtgcca gttatgaaat ggtgcaaaag tcggcgatgt gcggcgtaga gattttgttt
     7621 gcggtgtctg ccgcgaccac gcttgctgta gaagtggctg agcgttgtaa tctgacgctg
     7681 gtgggttttt gtaagccggg tcgggcaacg gtttataccc atccgcagcg tttgagcaat
     7741 taattaaaat aagtcctgcg atatat
//
LOCUS       DAPHDX010000173         7611 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPHDX010000173 DAPHDX010000000
VERSION     DAPHDX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.175"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..869
                     /gene="gatY"
                     /locus_tag="RH058_003994"
     CDS             15..869
                     /gene="gatY"
                     /locus_tag="RH058_003994"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV3139507.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            898..2160
                     /gene="gatZ"
                     /locus_tag="RH058_003995"
     CDS             898..2160
                     /gene="gatZ"
                     /locus_tag="RH058_003995"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDV3139508.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2170..2622
                     /gene="gatA"
                     /locus_tag="RH058_003996"
     CDS             2170..2622
                     /gene="gatA"
                     /locus_tag="RH058_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV3139509.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETKLKEYFTKYVLDSDE"
     gene            2653..2937
                     /gene="gatB"
                     /locus_tag="RH058_003997"
     CDS             2653..2937
                     /gene="gatB"
                     /locus_tag="RH058_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV3139510.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2941..4296
                     /locus_tag="RH058_003998"
     CDS             2941..4296
                     /locus_tag="RH058_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV3139511.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDTIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            4344..5384
                     /gene="gatD"
                     /locus_tag="RH058_003999"
     CDS             4344..5384
                     /gene="gatD"
                     /locus_tag="RH058_003999"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV3139512.1"
                     /translation="MKSVVNDTDGVVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVNRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            5490..6263
                     /locus_tag="RH058_004000"
     CDS             5490..6263
                     /locus_tag="RH058_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3139513.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6345..7244)
                     /gene="yegS"
                     /locus_tag="RH058_004001"
     CDS             complement(6345..7244)
                     /gene="yegS"
                     /locus_tag="RH058_004001"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV3139514.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1921 a         1705 c         1973 g         2012 t
ORIGIN      
        1 gacaggatat gaaaatgtac gtggtatcga caaagcagat gctgaacaac gcacagcgcg
       61 gcggttatgc ggttccggca ttcaatattc acaatctcga aacgatgcaa gtggtggtag
      121 aaaccgctgc caacctgcat gcgccggtca tcatcgccgg aacgcctggc acatttactc
      181 atgctggtac agaaaatctg ttggcgctgg tcagcgcgat ggcgaagcaa tatcaccatc
      241 cactggcaat tcatctcgac catcacacga aatttgacga tatcgctcag aaggttcgtt
      301 ctggcgtgcg ctcagtcatg attgacgcct cgcatttgcc ttttgcgcaa aatatttcac
      361 gggtcaaaga ggtggtggat ttttgccatc gctttgatgt cagcgtcgaa gcggagctgg
      421 ggcaacttgg cggccaggaa gatgatgtgc aagtcaatga agccgatgcg ttgtacacca
      481 accccgcaca ggcgcgtgaa tttgccgagg caaccgggat tgattccctg gcggtagcca
      541 tcggcacggc tcatggtatg tatgccagcg caccggcgct tgatttttct cgactggaga
      601 acattcgcca gtgggtgaac ttaccgctgg tgctgcatgg cgcgtcaggg ttatcgacta
      661 aggatattca gcaaaccatc aaactgggga tatgcaaaat caacgttgca acggagctga
      721 aaaatgcctt ctcgcaggcg ttaaaaaatt acctgaccga gcaccccgaa gcgaccgatc
      781 cccgggatta tttgcagtcg gctaaatccg caatgcgcga tgtggtgagc aaagtgattg
      841 ccgattgtgg ctgcgagggc agggcataac gcacctgcca tttaataagg aaaaaacatg
      901 aaaacgttaa ttgcccggca taaagctggt gaacatatcg gcatatgttc agtctgttct
      961 gcccatccgt tggttatcga agcggcgctg gcatttgatc gcaacagcac gcgcaaagtg
     1021 ctgattgaag caacgtcaaa ccaggtcaat caatttggcg gttataccgg aatgacaccg
     1081 gcagactttc gcgaatttgt ttttacgatt gccgataaag ttgggtttgc acgcgaacgc
     1141 attattctcg gcggcgatca tctggggcca aactgctggc agcaagaaaa tgcggatgcg
     1201 gcgatggaaa aatccgtcga gctggtaaag gaatatgttc gtgccggttt cagtaaaatt
     1261 catcttgatg cgtcaatgtc ctgcgcgggg gatcccatac cgttagcacc agaaacggtt
     1321 gcggaacgag ctgctgtgct ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt
     1381 gagcaactga gctatgtcat tggcaccgaa gttccggttc cgggcggtga ggccagcgcc
     1441 attcagtcag tacacatcac ccatgttgaa gatgccgcca atactttacg tacgcatcaa
     1501 aaggccttta ttgcccgtgg gctgacagag gcgttaacac gcgtgattgc catcgtggtg
     1561 cagccgggcg tggaatttga tcacagcaat attatccatt atcagccgca ggaagcgcag
     1621 ccgctggcgc aatggataga aagcacccga atggtttatg aagcacattc taccgattac
     1681 cagacccgga cggcttattg ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt
     1741 cccgcattaa cctttgcttt acgtgaggcg atatttgcgc tggcgcaaat tgagcaggaa
     1801 cttatcgccc ccgaaaatcg cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac
     1861 gaaccgcaat actggaaaaa atattatcgt acgggtttta acgattcatt actggatatt
     1921 cgttacagcc tgtcggatcg tattcgttat tactggccgc atagtcggat taaaaatagc
     1981 gtcgaaacga tgatggtgaa tcttgaaggc gtggacatcc cactgggcat gattagtcag
     2041 tatcttccca aacaatttga acgcattcag tccggggaat tatcagcaat accgcatcag
     2101 ctgattatgg ataaaattta tgatgttttg cgcgcctatc gctacggctg tgcggaataa
     2161 ggacggtata tgactaacct gtttgttcgt agcggaattt cttttgtcga tcgtagcgaa
     2221 gttttaaccc atatcggtaa tgagatgctc gccaaaggtg tggttcatga tacctggcca
     2281 caggcattaa ttgccagaga agcagaattc cctaccggga taatgcttga gcagcacgcc
     2341 attgcaatac cgcattgtga ggcgattcat gctaagtcgt cagccattta tctgttaagg
     2401 ccaacaaata aagttcattt tcagcaagcg gatgatgata acgacgtgga ggtatcgttg
     2461 gttattgcgt tgattgttga aaacccgcag cagcaattga aacttttacg ctgtttattt
     2521 ggcaagttac aacagcccga tatcgtcgag acactaatca ctcttcctga aactaagtta
     2581 aaggaatact tcacaaaata tgttttagat tcagacgaat aaatccctct gtaacaataa
     2641 aaaggactat ttatgaaacg caagattatt gtcgcttgcg gaggcgcggt tgcgacctct
     2701 acgatggcgg cggaagaaat taaagagttg tgtcagagtc ataatattcc tgttgaatta
     2761 atccagtgtc gggttaatga aatagaaacc tatatggatg gtgtgcattt gatatgtacc
     2821 actgccaggg tggatcgcag ttttggcgat attccgttag ttcacggcat gccttttatt
     2881 tctggtgtcg gtatcgaagc attacaaaat aaaattctga ctatcttaca ggggtgacct
     2941 atgttttcag aagtcatgcg ttatattctc gacctcggcc ctacggtgat gcttccgatt
     3001 gtcatcatta ttttttctaa aatattaggc atgaaggcag gcgattgctt taaagcgggt
     3061 ctgcatatcg ggattggctt tgttggcatt ggccttgtga ttggcttaat gctggattcc
     3121 attggtccgg cggcgaaagc gatggcggaa aatttcgacc tgaatctgca tgtggtcgat
     3181 gtcggctggc cgggctcttc accaatgacc tgggcgtcgc aaattgcgct ggtggcgatt
     3241 ccgattgcga ttctggttaa cgtggcgatg ttactgaccc gtatgacgcg ggtggtaaat
     3301 gttgatatct ggaatatctg gcatatgacc ttcaccggcg cgttgctgca tctggcaacc
     3361 ggttcatgga tgatagggat ggcgggtgtg gtaattcacg cggcgtttgt ttataagctc
     3421 ggcgactggt ttgcccgcga tacccgaaat ttctttgagc tggaaggcat tgctattccg
     3481 cacggtacgt cggcgtatat ggggccgatt gcggtgctgg tcgatactat catcgagaaa
     3541 atcccaggcg ttaaccgaat taaatttagc gccgacgata ttcagcgtaa atttggtccg
     3601 tttggcgagc ctgtcaccgt gggttttgtg atggggctga ttatcggcat cctcgcgggt
     3661 tacgatgtca aaggtgtatt gcagctggcg gtaaaaacgg cggcggtgat gctgctgatg
     3721 ccacgggtga ttaaacccat catggatggt ttaacgccca tcgctaagca ggcccgtagt
     3781 cgtttacagg cgaagttcgg cggtcaggag ttcctgattg gcctggatcc cgcattactg
     3841 ctggggcata cggcggtggt atcggcaagc ctgattttta ttccgctcac cattttaatt
     3901 gctgtttgtg ttccgggtaa tcaggtactg ccgtttggcg atcttgccac tatcggcttc
     3961 ttcgtggcga tggcggtcgc ggtgcatcgt ggaaatctgt tccgcacctt aatctcgggt
     4021 gtcatcatta tgagcatcac cctgtggatc gcgacgcaaa ctattggttt gcacacccaa
     4081 ctggcggcta atgctggggc gttaaaagcc gggggtatgg tggcttcaat ggatcagggc
     4141 ggttctccca ttacctggtt actgattcag gttttctccc cgcaaaatat tcccggtttc
     4201 attattatcg gcgcaattta tctgaccggt attttcatga cctggcgtag agcgcgtggt
     4261 tttactaaac aagagaaagc cgttctcgca gaataatttt tacctgaggg ggaggttatc
     4321 ccccttaaca atcaggagtt tttatgaaat cagtagtgaa tgatactgat ggtgtcgtgc
     4381 gcgttgcaaa aagcgtcatt cctgaaatta aacatcagga tgaggtgcgg gtaaaaattg
     4441 ccagctcggg cttatgtggt tccgatttac ccaggatatt taaaaatggt gcacattatt
     4501 atccaataac gttaggccat gaatttagcg gctatattga tgcggtagga tccggtgttg
     4561 atgatttaca ccctggcgat gcggttgcct gtgtgccgtt attaccctgt tttacttgtc
     4621 cagagtgttt gaaagggttt tattcccagt gcgcaaaata tgattttatt ggctcgcggc
     4681 gtgatggtgg atttgccgaa tatattgtcg ttaaccgaaa aaatgtcttt gctctaccca
     4741 cggatatgcc tattgaggat ggggctttta ttgagccgat taccgttggt ctgcatgctt
     4801 ttcatttagc gcaaggttgt gagaataaaa acgttattat tattggtgcc ggaaccattg
     4861 gcctgctggc tattcagtgc gctgtcgcgc tgggagcaaa gagtgtgacg gctatcgaca
     4921 ttagctcaga aaaactggca ctggcaaaat ctttcggtgc gatgcaaaca tttaacagta
     4981 gcgaaatgag cgcgccgcaa atgcagagcg ttttacgcga actgcgtttt aatcagctta
     5041 tcctcgagac ggctggcgta ccgcaaactg tcgaactggc ggtagagatt gccggtcctc
     5101 atgcccaact ggcgctggtg ggcacgttgc atcaggatct gcatttaaca tcggcaacgt
     5161 ttggcaaaat attgcgtaaa gagctgacgg ttatcggcag ttggatgaac tattccagcc
     5221 cttggccggg gcaggagtgg gaaacggcga gccggttgct gacagaacgt aagttaagcc
     5281 tggagccatt aatcgctcac cgtggaagct ttgaaagctt cacccaggcg gtgcgtgaca
     5341 tcgctcgtaa tgccatgccg ggcaaagtgt tgctcattcc atgaaaccgc gggccagcgt
     5401 gatgctggcc cggtattgtg caaaacagat cattcaccaa tggtccccct tcgtttacac
     5461 tagccacaat tgaatatggg taaatgacga tgaattcatt cgagcgaagg aataagatca
     5521 tccaattagt gaatgaacag ggaaccgtgc ttgttcagga tctggcggga gtatttgctg
     5581 cctcggaagc gacaatccgt gccgatttgc gctttctcga acaaaaaggc gtggttacgc
     5641 gctttcatgg cggtgcggcg aaaataatgt ccggtaatag tgaaaccgag acccaggaag
     5701 tcgggtttaa agagcgattt cagctcgcca gcgcgcccaa aaacagaata gcgcaggcgg
     5761 cagtcaaaat gatccatgaa gggatgaccg ttattctcga cagcggaagt accacaatgc
     5821 ttatcgccga aggattaatg accgccaaaa atatcacggt gattaccaac agtctcccgg
     5881 cagcgtttgc cctttccgaa aataaagaca ttactctggt cgtctgtggt ggcacggtcc
     5941 gccataaaac gcgctcgatg catggttcca ttgccgagcg ttcattgcag gatattaatg
     6001 ccgatttaat gtttgtcggt gctgatggta ttgatgcggt taatggtatt acgaccttta
     6061 atgagggtta ttccgttagc ggtgcgatgg tcacagccgc taataaagtg attgccgttc
     6121 tcgattcatc gaaatttaac cgtcgcggtt ttaatcaggt actgccaatc gaaaaaattg
     6181 acattattat taccgatgat gctgtgtctg aggtggataa actggcattg cagaagacac
     6241 gggtgaaatt aattacggtg tagtattgtt ctgtatgccg gataaggtat ttacaccgca
     6301 tccggcatta cggattctcc ctggttcaag agagaatagt ttgattaacg caataatgga
     6361 caatctggtg gtaatcgaca acgcaacgcc gccggaagta tttcaatatg aaagttttgt
     6421 ccgctcaacg gttcgccatc aagattaaaa gtgatttcgt gtggggcttg tatatcaaac
     6481 cacgacgaag cgccttcgat aatattcggg ttatcttcgt cagattttaa ggttgatacg
     6541 agagccggaa gaatttcatc gccggtaaaa atgcgcagtt gcagcaagcc atcgttaatt
     6601 aacgcgttcg gacacaattg ctgaccgcca ccggcctgac gcccgttacc aataccaatg
     6661 accagggcgt caccttgcca gtgaaagttt tcaccgcgga tttcacaacg gtccggttgc
     6721 agagtatcca tgcgcattaa gccatgaatg atgtaagaga cgccacccag cgcggctttt
     6781 aatttttccg gcgtttctgt ggtaatacgc gtcccaaatc cgcctgtcgc catattaata
     6841 aaacaggttt gtttgttgac ctgcgccata tctatcgcaa tggcgttacc ggcaattgcc
     6901 agtttcagcg ccttatccag tgcctcagga atccctacac tggtggcaaa atcattggcg
     6961 gttcctaatg gcaaaattcc cagcgcgggt atgtcatccc cctcacactg aatcaacgcc
     7021 gtagaaactt cattaatggt gccatcgcca ccaccggcaa tcaccgttgc gacgcccaac
     7081 ttccgggcct cctctacata tcgtgcggca tcgcctttct cccaggtgac ccgcacatgg
     7141 atcgtcattc cttcctcacg caacagcata attgcttcgc gcaagggtag attgtcagta
     7201 cttttgccat taagaatcag taagctggcg ggaaattctg ccatgatcgt atgtgccttt
     7261 atgattggtc tgtagatagt gtagagcaga aaacaaaagg tggagtcaga acaggatgaa
     7321 agtcggagga taaaaagtca gttcaagtat ggaggattca gtggttacca ccaggagctt
     7381 gcaatgggga cagcgaatac taacggctgc caccgataaa tttcaaaaaa gagcatatgc
     7441 ctaatattca accaaacagt ggcatcttaa atatactatt ccaaatatat taaagccccc
     7501 atggagttac cctgaagggc ctcaatgtcc gtaattccta cttatgtagg aaatgttgta
     7561 cagaacattt attataatcc tattcaatta taataatcat gccattatta t
//
LOCUS       DAPHDX010000174         7514 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPHDX010000174 DAPHDX010000000
VERSION     DAPHDX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.176"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..790
                     /locus_tag="RH058_004002"
     CDS             50..790
                     /locus_tag="RH058_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV3139515.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(812..1000)
                     /locus_tag="RH058_004003"
                     /pseudo
     CDS             complement(812..1000)
                     /locus_tag="RH058_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1004..1105
                     /locus_tag="RH058_004004"
                     /pseudo
     CDS             1004..1105
                     /locus_tag="RH058_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1137..1832)
                     /gene="araD"
                     /locus_tag="RH058_004005"
     CDS             complement(1137..1832)
                     /gene="araD"
                     /locus_tag="RH058_004005"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV3139516.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1826..2686)
                     /locus_tag="RH058_004006"
     CDS             complement(1826..2686)
                     /locus_tag="RH058_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDV3139517.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2679..3341)
                     /gene="ulaD"
                     /locus_tag="RH058_004007"
     CDS             complement(2679..3341)
                     /gene="ulaD"
                     /locus_tag="RH058_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV3139518.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHHGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3338..4834)
                     /gene="lyxK"
                     /locus_tag="RH058_004008"
     CDS             complement(3338..4834)
                     /gene="lyxK"
                     /locus_tag="RH058_004008"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDV3139519.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4838..5824)
                     /gene="yiaO"
                     /locus_tag="RH058_004009"
     CDS             complement(4838..5824)
                     /gene="yiaO"
                     /locus_tag="RH058_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDV3139520.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(5837..7114)
                     /gene="yiaN"
                     /locus_tag="RH058_004010"
     CDS             complement(5837..7114)
                     /gene="yiaN"
                     /locus_tag="RH058_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDV3139521.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(7117..>7514)
                     /gene="yiaM"
                     /locus_tag="RH058_004011"
     CDS             complement(7117..>7514)
                     /gene="yiaM"
                     /locus_tag="RH058_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDV3139522.1"
                     /translation="RYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHVQVTFLVE
                     KLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPT
                     SLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT         1771 a         2019 c         2012 g         1712 t
ORIGIN      
        1 attactatgc cgactctttt ttatcttatt ttaaaatgga ataccggaca tgttaattaa
       61 ccgcaatatt gtggcgttat ttgcgttgcc ttttatggca agcgcaactg cttctgaatt
      121 atccattggt gctggtgcgg cttataatga atcgccttat cgcggttata ataaaaatac
      181 aaaggcaatt ccgctgatta gttatgaagg tgattctttt tatgttcgtc agaccacgtt
      241 aggttttatt ctgtcgcaaa gtgaaaaaaa tgaacttagc ctgaccgcat cctggatgcc
      301 gctggaattt gaccctgccg ataatgacga ttatgccatg caacagcttg ataagcgtga
      361 tagtacggct atggcggggg ttgcctggta tcaccacgag cgttggggaa ccgtgaaagc
      421 ctctgcggct gcggacgttc tggataacag caacggctgg gtgggggagc tatcggtatt
      481 ccacaaaatg cagataggtc gcctgtcgct gacacctgcg ctgggcgttc tctattatga
      541 cgagaatttc agtgactatt actatggcat ttcagagagt gagtcccgtc gtagcggtct
      601 ggcaagttat tccgcgcagg atgcctgggt gccctatgtc agcctgacgg caaaataccc
      661 gataggagag cacgtcgtat tgatggcgag cgcaggatac agcgagctgc cggaagagat
      721 taccgacagc ccgatgattg atcgtaatga gagttttacc tttgtcaccg gggtgagctg
      781 gcgtttttaa ttcaccggtg gatgtcggtg cggctggtgc tccgtcgtaa tgccacaaag
      841 gttgttgctc tggattgctc tgttcatacc gggaacctgg cgtctgaatc aggatcttat
      901 ggctgaacaa tcccttgcct tcaaatgtcg taaatggcgc atcggcgggc agcaacaggt
      961 tatgcagtga ctcgacgttg tcggcctgct ccatgcaata cggtttaatg acgttggcta
     1021 tttccggcag atttttcaga aacatactgg tttaacgcca gcagcgtgga agcggcgtta
     1081 cagcaaggaa catatcaatt cgtagtgccg gggcgatgaa gccccggcgt gagggattac
     1141 tgcccgtaat aggcattggc cccatgctta cgcaggtagt gcttatccag cagttcgttt
     1201 tgcatcgcag ggagctgcgg cgcaagctgg cgcgagaata gacccatata ggcgcattct
     1261 tcgagtacta cggcgttatg cacggcatcg gcggcgtttt taccccatgc gaacgggccg
     1321 tgagaatgca ccagcaccgc cgggatttgt gccggactcc tgccacgttc ttcgaaggtt
     1381 tcaatgatca cttcgccggt ctgatattca tattcgccgt taatctcctc tgcggtcatc
     1441 tgtcgcgtgc aggggatagc accgtaaaaa taatcggcgt gggtggtgcc ccaggcgggg
     1501 agatccagcc cggcctgtga ccagatggtg gcgtggcgcg agtgggtatg cacaatgcca
     1561 ccaatttcgg catagcgacg gtaaagcgcc agatgcgttg gcgtgtcgga cgagggcttt
     1621 ttgctacctt ccaccacctt gccgctggct atctcaacca ccaccatatc gtcggcggtc
     1681 atcacgtcgt actcgacgcc ggaaggtttg attaccatcc attgccgcgt gtcgtctacc
     1741 gcgctgacat tgccccaggt gaacgtcacc agatggtgag cgggaagcgc cagattcgcc
     1801 gccagcacgt cggctttcag ttgctctaac atataaatcc agcctcctgc atacgcgctt
     1861 caatccagcg ccgcgcctga atgatctcca gcaccggctc tttggctttt tcggtccaca
     1921 tctcaatcag gaaagaaccg cgatagttca gttcatgcag cgttttgaag atgccaacga
     1981 aatcgacgca gccttcgcca aacggcacat cacggaactg tccggggctt tgcccggtga
     2041 ctggctgggt gtctttcagg tggatcgcgg cgatacggtc aatgcccagt ttcagttcgg
     2101 cgggaacatc attgccccag gcgctgaggt tgccgacgtc cgggtagacg gtgaaccacg
     2161 gcgaggcgag catctcgtcc cattttttcc atttgctgat ggagttcata aacgctgtat
     2221 ccataatctc caccgccagc attacttgtg atgccgccgc ctgttcgact gcccacgcca
     2281 gcccttcagc aaaacgttgc cgggtgcctt cgtcgtggtc ttcgtaatag acgtcataac
     2341 ctgccagctg gatggtgcga atgccgagat cgcgcgccag gcgaatcgct ttactcatga
     2401 tttcccgcgc ccgttcgcgc actgcctcgt cacggctacc aaaaggaaag cgacgatgtg
     2461 cggacaggca catcgacggg atcccaacac ccgtttcgat catcgcggca accagcgaag
     2521 tcctttgtgc ggtgctccag tcaagacgtg aaagccgttc gtcggtttca tccaccgaca
     2581 tttcgacaaa atcaaaaccg cagctttttg ccagcaccag ccgctccggc caggagagat
     2641 ctttcgccag cgccttttca taaatcccta acggatgatt acgcacgctt gcctccccag
     2701 atagcgtcga tttgcgcatg gaaatcgcca gccacctgcg ccggatttgc tgagcctgcc
     2761 agtgctcgcc cggcaataaa cgctttcacg cgaatatctt taaacagcgg caggtcagca
     2821 ggggtaatcc cgccggtaat ggaaagctca aggccgatat cggaaagcgc cttcatgcgt
     2881 gccagatcgg cttcgcccca ctgttgcccg ctggcctgtg catcacgacc gtgatgataa
     2941 atcgcctgcc gcacgccaat acgatgccag tcgcgggcgt cgtccagcgt ccagttaccg
     3001 aacagctcga tctgaatttc accaccgcag cgttgcgcca tcgcgtggcc tttttccacc
     3061 gtcgcgagcg gtgcggcgca gatgatggtc atccagttag cgcctgcgcc aaatgcctgt
     3121 tgcgcgagcg tttcaccagc gtcggcgacc ttccagtcgg caacgatgat tttgtcaggg
     3181 cactgttcgc gcaaggcttt caccgcgcca agcccttcgt ttaaacagag aatggtgccc
     3241 gcttcgacaa tatcgacgct gtcttttaac tgcgtcacgt cgcgctgtgc ggcttcaagt
     3301 gatgagtggt cgagtgccag ttgcagaagt ggtcggctca taatatgtgc tccttgatgc
     3361 gggcgtgaaa gccctgaagt gcggcaatga gatgctgata gcgttggtat ttttgttggt
     3421 aaagctgatg ggcggtcata tccggcagca gggtgcgtac cgggtgctgt aagtcacgtt
     3481 gagcttcgct gaaatcgcga taaactccgg tgccgacacg ggcggcaagt gccgcgccaa
     3541 aacagccggt ttcttccacc tgcggcagct cgatacgcag accgctgacg tccgccagca
     3601 tttgcatcca gacatcggag tgcgccgggc cgccagtgac gcgcagggtg tgcacatcag
     3661 taaaacgttc gcgcatccgg ttgaggtggg tcatatggct gaacaccacc ccttcataaa
     3721 tggcctgcaa caggtgtgcg cgggtgtgaa tggcctgcat cccgtagaaa ccgctggtca
     3781 tctcgagtcc ggcgttgctg ccgtacagga acggcaggaa aaagagatcg ccaccggcct
     3841 tcggcaagct ggcaacggcc tggttgatct catcaaacga gatttctccc cactgtgcgg
     3901 taaaccattc gaggttgccg gaagaggtgg ggctggcttc gtgaacgata aattcaccat
     3961 cgttaacgta gcgaccatag acatacggat gcgcttcacc gtcacgtaaa ccgcgggtta
     4021 tgccgctggt caccgcccag gtgcccatca ccgcattaag ggtaaattcg tcttcgatcc
     4081 cggcgcagag tgcggtggaa accacatcaa acaggccgcc gacaacgggc gtacccgctt
     4141 tcagaccggt cagtacggct gtctgagcgg tgatctcccc gcagatttcg gcagatccga
     4201 caacaggcgg cagggcgtga ttgatttcag caatccccag ccagtcggtg aggcacgggt
     4261 catattcccc aagactcatg ttgtagaggt tggactcgga aatattgctc tcttcacagc
     4321 ctttgacgcc ggttaaacac cagcgcaggt agtcgtgcgt catcatcacg cagccaattt
     4381 gcgcgtagcg ttccggttcg tgctctttca gccagcgtaa cagcgacacc ggatgcccgg
     4441 tccacaaggt ttgtcgggtc aacgggtaga gtttttccgg gatgccatct tcctgccagc
     4501 gacgaacgat ttccatcgcc cggcgatccg aggacaaaat ggcgttaccg agcggcttgt
     4561 tgtttttatc cagcaaaaac aagccttttc cttgtgcgga gataccgata ccgacaattt
     4621 gttccccgct aacgccggaa tgggtaagca gttcgcgaat gacagccatg cagcattgcc
     4681 acagttctgc catatcgcgc tctgcccaac ctggctgcgg gcttaatgcg cacagcggca
     4741 ggcgctgcac gcctgcctcc cggccttcgc ggtcatacag cccggctttc agccagctac
     4801 cgccacaatc taaccccagc cagtattgcg tcatggttta ttgcacctca tccacggctt
     4861 tcagtagatc agcaccctgc ggaacatctt ttacaaacat gtcgcggacc tgagtaccca
     4921 gtgcgtcgct aaaggctttg cggtcgagat cggtgatgac ttccacgccc gcttctttca
     4981 tgccgtcgat gattttttgc tgatcttcgg caaccagttt gcgctggtag ttacccgctt
     5041 cctgcgcaga tgaaattagc gcttgctgga actccgggct taagccatca aacttcgctt
     5101 tgttgatcac caccagaagc ggggaatagg cgtggtgcgt cagagaaagg tacttctgca
     5161 cttcgaaaaa ttttgctgac cagacgacgt tgatcgggtg ttcctgagcg tcgatagtgc
     5221 gcgtttccag cccggtatag acttcggcaa atggcatcgg gatcgggtta gcgccaaaga
     5281 ctttgaatgc ggcgatattc atcgggctat tgttggtgcg aattttcagc cctttcaggt
     5341 cggcgggggt tttaaccggt gcgcgcgagt tggtgacatc gcgccagccg ttttcccagt
     5401 aggccagtac tttcagtcct ttaccttcaa gtgaggcttt cagatcatca ccgactttgc
     5461 cgtcgagcgt tttatgcgcg tgagcggtat cgcggaacag gaaagggaca tcaagcaagt
     5521 tcatcactgg tgataacccg gcaaagttat tcgagccgga catttccata tcgatggtgc
     5581 cgccacgtac gccgctgatc atcgcctgcg cgttaccgag ggtgctatcc ggaaacaatt
     5641 ttaatttcag ctcgcctttg gttctctcct gcaataaatc attgaatttt ttcgccgcaa
     5701 tatgttgcga gtcggtttgt gaggtttcat aaccgaaacg taaagattgc gccgccagag
     5761 aagatgtgct gaatgcagcc aggccagcaa tgaataatgc gtaggttaca gagcgtaatt
     5821 tcataatatt ttccttttaa ttaatccatt tcaaagggag gatgatgaga tcgggaataa
     5881 aaacaaatac gactaataat gaatataaaa ccaggacgta agggaaaacg cctctgactg
     5941 catcatcgaa tttgagtttt gccacccctg aaataacgtt aagtacatta ccgataggcg
     6001 gtgtaattaa gccgattgag cagttaatga taaacatgac accgaaataa atcggatcga
     6061 ttcctgcctc tttaactaaa ggcattaata ccggggtaag aattaatacc gtcggcgtta
     6121 aatccatgac catgccgaca atcaggatag ccaccataat gacgataaac agcagacgcg
     6181 gtgaatcgac caacggttgc agtaaatcag aaaccatcat cggcagttca gcaatggtaa
     6241 tcagccaggc ggaaacttgt gccgaggcca ccagaaacat aaccactgag gtggttttcg
     6301 ccgcgccaat gagtacatgc caaagtgtgg cgaaggtcat ttcacggtaa ataactgtgg
     6361 cgacaaacag cgcatagaaa gcagcaacgg ccccggcttc ggtaggggta aacagaccgg
     6421 agcgaaaccc gccaataatg atgactggaa gaaacagcgc ccagataccg gagacaaaag
     6481 agtgccagat ttcctgcatc gttgcttttt gctggcgggg taaattaagg cgactggcct
     6541 gccaccacca ggtaagcatc agcgttgcgc ccatcatcat gccaggggca atgcccgcca
     6601 taaacagctt actgatggat aatccgctgg aaacgccgaa gataataaac ggaatggaag
     6661 ggggaataat tggcgcgata atgccgccag aggcaatcag ccccgccgcc cggttgaccg
     6721 ggtagttggc gctgcgcatc atcggcacca gtagagcggc aacggcggcg gtatccgcaa
     6781 cggcagaacc agaaaggctc gccataatca tagctgccag cacgccgacg tagcccagcc
     6841 cgcctggttt atgccccacc agtttcatcg gcaggtcaac aatgcgtttt gacaagccgc
     6901 ccgcattcat gatttcaccc gccagcacaa agaacggaat cgccagcagg gagaagctat
     6961 cggctccgtt caccagcgtt tgtgccatga tctggacatc aaacatgtcc agccagaaca
     7021 ttaacgccgc cccgcacaac aacagtgccc aggcaatagg caaaccgata gcaataccac
     7081 ccaacagaca gcccagaaaa atcagcacag ccatgattaa gctccttgcg gtggcgacgt
     7141 tagagaattg ctacgcgtga tgagttgata taaatgacgc agttcaaaga atgcgataac
     7201 gaggctggtg ggcagacaag cggcatacat caggccgatg ggtaaaccga ggatcggtga
     7261 ataatcgctc cagtcctgaa ttgtttttag cgttgcgccc caggccagtg cgccacaaat
     7321 aaataagatt aaggaatggg taaccagagc gactcgtcgt tgccatgcgg gggagagttt
     7381 ctccaccaga aaggtgacct gaacgtgggc gttatccata aaagccacaa tcgcgccaat
     7441 aaatgttaac cagacaaata aataacgtga caattcatct acagataaaa tgcttgtctg
     7501 aaaaccatat ctta
//
LOCUS       DAPHDX010000175         7486 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPHDX010000175 DAPHDX010000000
VERSION     DAPHDX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.177"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(93..916)
                     /locus_tag="RH058_004012"
                     /pseudo
     CDS             complement(93..916)
                     /locus_tag="RH058_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(970..2688)
                     /gene="proS"
                     /locus_tag="RH058_004013"
     CDS             complement(970..2688)
                     /gene="proS"
                     /locus_tag="RH058_004013"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV3139523.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2800..3507)
                     /gene="tsaA"
                     /locus_tag="RH058_004014"
     CDS             complement(2800..3507)
                     /gene="tsaA"
                     /locus_tag="RH058_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV3139524.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3504..3908)
                     /gene="rcsF"
                     /locus_tag="RH058_004015"
     CDS             complement(3504..3908)
                     /gene="rcsF"
                     /locus_tag="RH058_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV3139525.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4026..4841)
                     /gene="metQ"
                     /locus_tag="RH058_004016"
     CDS             complement(4026..4841)
                     /gene="metQ"
                     /locus_tag="RH058_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV3139526.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4881..5534)
                     /gene="metI"
                     /locus_tag="RH058_004017"
     CDS             complement(4881..5534)
                     /gene="metI"
                     /locus_tag="RH058_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV3139527.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5527..6558)
                     /gene="metN"
                     /locus_tag="RH058_004018"
     CDS             complement(5527..6558)
                     /gene="metN"
                     /locus_tag="RH058_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV3139528.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6746..7321
                     /gene="gmhB"
                     /locus_tag="RH058_004019"
     CDS             6746..7321
                     /gene="gmhB"
                     /locus_tag="RH058_004019"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV3139529.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1842 a         1933 c         1900 g         1811 t
ORIGIN      
        1 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
       61 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
      121 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
      181 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
      241 tgcgccagtg taagtggatg cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa
      301 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
      361 acgcactttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
      421 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
      481 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
      541 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
      601 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
      661 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
      721 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
      781 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
      841 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaaacagg cggcagtacg
      901 cctttggttt atccatttta tacaatccat gtaaaaaaag ggccctgaaa ttcaggaccc
      961 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
     1021 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
     1081 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
     1141 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
     1201 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
     1261 cacctggaac ggcgcgatag cgtcaggcca tacgatgcct cgttcgtcgt agttctgctc
     1321 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
     1381 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
     1441 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
     1501 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
     1561 atcccagttg atgccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
     1621 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
     1681 acccggaccg gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
     1741 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
     1801 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
     1861 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
     1921 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
     1981 tgccagttca atgttagctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
     2041 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
     2101 ttgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
     2161 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
     2221 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
     2281 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
     2341 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
     2401 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcagc
     2461 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
     2521 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
     2581 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
     2641 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
     2701 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaaagt gatttagttt
     2761 accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa
     2821 gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc
     2881 ataggttttg cccgtttcct cacctttacg ataggccggg cgtgggtcct gcgccagtac
     2941 ttcgcggata aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc
     3001 gacctctgcg gtaaaactca ccgccatctc tgccgctggc gcactttgcg catagctggc
     3061 actggcatcg ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt
     3121 accatcgacc agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac
     3181 ctctttcagc tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc
     3241 gaaaaccccc attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc
     3301 gccttccatc gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc
     3361 gcgaacggcg tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt
     3421 taccagacct ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc
     3481 tatttgctcg aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat
     3541 acagcctgac gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc
     3601 gcattggcct tcattttcga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc
     3661 ggcggagagt cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg
     3721 aacggtttgc cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc
     3781 gctttcggtt ttgcaggctc cgctttcggc tggggtgcag tgctttgaac gggttcgaca
     3841 ggggatctgc ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa
     3901 gcacgcatag tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta
     3961 aaaatgacaa gacgggcaag cgcccgtcct gaatgatatt acaaattgtg gaaacagcct
     4021 aaaaattacc agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact
     4081 tcatcagact gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca
     4141 cgcgttacga tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc
     4201 gctggagtca ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgc
     4261 gcgtcgtcca gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt
     4321 gggttctcaa caacatcaag aacggtcggc agcaggccaa cgccatcttt cagtttgatc
     4381 aagcccactt tttgcagcag cagcagtgaa cgaccaagat tagttggatc gtttggcacg
     4441 gcaacctgcg aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca
     4501 atcggataaa caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc
     4561 tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct
     4621 tcgtttggca gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct
     4681 ttcgcaactt tctgcgcgac ttctgctacc tgctgttcgg caccaacaat cacgccgact
     4741 ttaatgtggt ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc
     4801 agggctccca ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat
     4861 tatttatgtt gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg
     4921 aactgaatta aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg
     4981 ttatagccga tgtagccata ctgatagcca atctgaccta aaccaccggc accgactgca
     5041 ccacccatcg cggaataacc gaccagggta atcagggtga tagttgccgc attcaccaga
     5101 cccggcaatg cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt
     5161 gcacgggaag cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg
     5221 gcaataaacg gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat
     5281 gtaccgacaa taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggatg
     5341 gaacggaaaa tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt
     5401 tgccccgggc gcgtaacata aagcaggacg ccgaccggca gaccaatcac aaagccaaaa
     5461 aatccggata cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag caaccacatc
     5521 atcggctcag acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat
     5581 ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac
     5641 gccaccggcg taatccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc
     5701 ggtttcagaa agcagcgggg catcgaccga ttgaccggta aactccaggc gcaacatggg
     5761 cacgcagtca gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag
     5821 atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc
     5881 acttaccgtg tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat
     5941 acgcttcaca acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt
     6001 aatgtctttc agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc
     6061 ttcatcacac agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg
     6121 tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa
     6181 tgacagcaat tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag
     6241 cggcagagcc acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat
     6301 cataccaatc tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg
     6361 gccatcgacc agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag
     6421 cgtactctta cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg
     6481 caggctgacg ttgttcaacg cctggatggt gcgggtgccc tggtggaaca ctttggtgat
     6541 attcgaaagt tttatcattg attatttatt atcgtcatta agttagtcgt ggcatctcga
     6601 atgcctgaaa cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat
     6661 caatcaggtt tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtccc
     6721 atgccttatt aaggagctat aaaaggtggc gaagagcgta cccgcaattt ttcttgaccg
     6781 tgatggcacc attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttat
     6841 cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg ggctttgcgc tggtggtagt
     6901 aaccaaccag tctggcattg ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac
     6961 cgagtggatg gactggtcgc tggcggaccg agatgtcgat ctggatggta tctattattg
     7021 cccgcatcat ccgcagggta gtgttgaaga gtttcgccag gtctgcgatt gccgcaaacc
     7081 acatccgggg atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta
     7141 tatggtgggc gataaattag aagatatgca ggcagcggtt gcggcgaacg tgggaacaaa
     7201 agtgctggtg cgtacgggta aacctattac acctgaagca gaaaacgcgg cagattgggt
     7261 gttaaatagc ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcgcaatg
     7321 attaaaagat gagcggttga aataaaaatg catttttccg cttgtcttcc tgagccgact
     7381 ccctataatg cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt
     7441 cggttgaaga gaaaaaatcc tgaaattcag ggttgactct gaaaga
//
LOCUS       DAPHDX010000176         7471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPHDX010000176 DAPHDX010000000
VERSION     DAPHDX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.178"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            355..729
                     /gene="tomB"
                     /locus_tag="RH058_004020"
     CDS             355..729
                     /gene="tomB"
                     /locus_tag="RH058_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV3139530.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            755..973
                     /gene="hha"
                     /locus_tag="RH058_004021"
     CDS             755..973
                     /gene="hha"
                     /locus_tag="RH058_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV3139531.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1144..1695
                     /gene="maa"
                     /locus_tag="RH058_004022"
     CDS             1144..1695
                     /gene="maa"
                     /locus_tag="RH058_004022"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV3139532.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1811..2281
                     /locus_tag="RH058_004023"
     CDS             1811..2281
                     /locus_tag="RH058_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV3139533.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2445..3995
                     /gene="pdeB"
                     /locus_tag="RH058_004024"
     CDS             2445..3995
                     /gene="pdeB"
                     /locus_tag="RH058_004024"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV3139534.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4037..4390)
                     /locus_tag="RH058_004025"
     CDS             complement(4037..4390)
                     /locus_tag="RH058_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HDV3139535.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4734..4961)
                     /locus_tag="RH058_004026"
     CDS             complement(4734..4961)
                     /locus_tag="RH058_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDV3139536.1"
                     /translation="MGKDQHVVPHDGGWAVKGAGNSKATSVHSTKQSAVDAARGIATN
                     QKSELVIHNKDGRIAKKDSHGNDPCPPKDKK"
     gene            complement(5179..5799)
                     /locus_tag="RH058_004027"
     CDS             complement(5179..5799)
                     /locus_tag="RH058_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3139537.1"
                     /translation="MRVWAYLRASTKEQDADRALKELEAFAHSHDLKISKYFKENESG
                     ASLQRPQLFLLLDIAERGDILLCEQIDRISRLTAEDWKTLRGLIESKGIRVVSLDLPT
                     SHQLLHVQDEFTVRMFEAMNSMMLDMLAAISRKDYEDRRRRQKQGIEKAKREKKYRGR
                     PVDESLHHKVQELLSDGKSWSKIQALIGCSRATIAKVAKNSSLTAE"
     gene            6185..7237
                     /locus_tag="RH058_004028"
     CDS             6185..7237
                     /locus_tag="RH058_004028"
                     /inference="COORDINATES: protein motif:HMM:NF016159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDV3139538.1"
                     /translation="MSELKVISHAMFNISVEQAEASRVDLEHGEGDFQKYCGQLLDEL
                     LENTRSKSFKFRAIEEFVPAHLNVLVNDQNEWDKRTLGIAEKLLSVEIDAQDKIKAMK
                     KKIKKGALLILLLSRDNNFNFVILKIEHSDFFDEIESKIKKGLPLNRQRLQKSCLVSF
                     SNTYDVEEILISDSGASISEYWWKNFLSTVELQSSELNTKNAFGSIENFLKREVEKHS
                     SVDYWYMRNDIIAYFRNNEQFAYDELVEKVRSHKPESPAIDERFDQIIEKFKALPDHK
                     NSKFDRQFQLEPNVIRARIKKTIALSDNIELRINGEVADFRKKIVPESDALGKYLKIY
                     SDTGYEEFSAGKEDNV"
     gene            7234..>7471
                     /locus_tag="RH058_004029"
     CDS             7234..>7471
                     /locus_tag="RH058_004029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139539.1"
                     /translation="MNIVSKIADLFMPSQEVSSCDNFHLFKAEYRILETTIHQDYSQR
                     VEELTNEVIANNLDIEIDFKFETCDSFTLLISPEY"
BASE COUNT         2112 a         1632 c         1564 g         2163 t
ORIGIN      
        1 tcatttagag gctattctta attatttgcg gtaattcttt attcattcct cggttattac
       61 gtcatattca gagcaatcct ggtattagtg tcaccaattt catctggcga taatcctgaa
      121 atgttatgaa cagttcgagc agactgcttt tacctgctgc gggttagtgc tagtatgaaa
      181 aagtgagtcc tgtcccgatt ccttcctaat tgtaattttt cgtaataatg cgatgaaaac
      241 ctgcaaagag tggcttatag ttaagctaac aaacgagagg gcaagtccag gtcagtaagt
      301 tttttccatc ccgaaaggtg tccgttagtt caaccgctaa gaaggggacg cgttatggat
      361 gaatactcac ccaaaagaca tgatatcgca cagcttaagt ttctctgtga aaccctgtat
      421 catgactgcc ttgcaaacct tgaagaaagc aatcatggct gggttaacga cccaacctcg
      481 gcgatcaacc tccagttgaa tgaactgatt gagcatattg cgaccttcgc acttaattac
      541 aaaattaagt ataatgaaga caataagctc attgagcaga tcgacgaata tctggatgac
      601 acctttatgt tgttcagtag ttatggtatt aatatgcagg atcttcagaa atggcggaag
      661 tcaggtaatc gactattccg ttgttttgtc aatgcgacga aagagaatcc tgcgagttta
      721 tcttgttaga attattacaa ccatgggtag aagtatgtcc gaaaaacctt taacgaaaac
      781 cgattattta atgcgtttac gtcgttgcca gacaattgac acgctggagc gtgttatcga
      841 gaaaaataaa tacgaattat cagataatga actggcggta ttttactcag ccgcagatca
      901 ccgcctcgcc gaattgacca tgaataaact atacgacaag atcccttcct cagtatggaa
      961 atttattcgc taataaataa ttcgctttcg gagctataac cggctgttta ttaagaattt
     1021 tatacttttc accatgaaga cataccctat gtgatcttta tcacacagat gtaatgggaa
     1081 cgttctcttc actgactttt cgtcttactg tgttgccgca ttttcagcaa ccggaggtca
     1141 gtaatgagca cagaaaaaga aaagatgatt gctggtgagt tgtatcgctc ggcagatgag
     1201 acgttatctc gcgatcgcct gcgcgctcgt cagcttattc accgatacaa tcattccctg
     1261 gcggaagagc acacattacg ccagcaaatt ctcgctgatc tattcggtca ggtgacagag
     1321 gcttatattg agccaacgtt tcgctgtgat tatggctata acatttttct cggtaataat
     1381 tttttcgcca acttcgattg cgtgatgctt gatgtctgcc ctattcgcat cggtgataac
     1441 tgtatgttgg caccaggcgt tcatatctac acggcaacac atcccatcga ccctgtagca
     1501 cgtaatagcg gtgctgaact ggggaaaccc gtcaccatcg gtaataacgt ctggattggc
     1561 ggacgcgcgg tcattaaccc tggtgtgacc attggtgata acgtcgtggt agcctcaggt
     1621 gcagttgtca caaaagatgt cccggacaac gttgtcgtgg gcggtaatcc agccagaata
     1681 attaaaaaat tgtaatcggt ttttcgcaac tgttatgcaa aattgtggta aatctgttac
     1741 ttcctctcta ctattcccac gttaaaatag ggtgttccct ggaaagttgc agataccacg
     1801 aaggcaaacg atgaccgaaa tacaacgcct gctgaccgaa acgattgagt ctctgaatac
     1861 ccgcgaaaaa cgcgacaaca aaccccgctt tagtatcagt tttatccgta aacatccggg
     1921 gctgtttatc ggtatgtacg ttgctttttt tgccaccctg gcggtgatgt tgcagtccga
     1981 aacgctgtca ggctctgtct ggctactggt tgtattattt atcctgctta atggtttctt
     2041 ctttttcgat gtctacccac gctaccgcta tgaagatatc gacgtgctgg atttccgcgt
     2101 ttgctataac ggcgaatggt acaacacgcg ctttgtacct gccgcgctgg ttgaagccat
     2161 cttgaactct ccgcgtgtcg cggatgttca taaggaacaa ctgcaaaaaa tgatcgtccg
     2221 taaaggtgaa ctgtcttttt acgatatttt taccctcgct cgcgccgaat caacatctta
     2281 agttagggtt acataccagg cgtaaagctc tgcgcctggt caaatgacaa tgatcgtttc
     2341 cacccatcac ttcatgaaat accagctcta cctccttatc tcctgccagc ctttttccac
     2401 aatcagatat actttcccta cactgtgtta ataaggatat gctggtgaga acacgacatc
     2461 tggtcggcct tatttcggga gtactgattc tttcagtatt gctgcctgtc ggcttaagca
     2521 tctggctggc ccatcagcag gtagaaacat cgtttattga agagctggat acctattcct
     2581 cccgcgtcgc tattcgggcc aataaggtgg cgacacaagg gaaagatgcg ctgcaagagc
     2641 tggaaagatg gcaaggcgct gcctgtagcg aagcccatct catggaaatg cgtcgggtat
     2701 cttacagtta tcgctatatt caggaagtgg tttatatcga taacaacgtt ccccagtgtt
     2761 cgtctctgga gcatgaaagt ccgcccgata ccttccccga gccaggtaaa atttcgaaag
     2821 atggttatcg tgtctggtta acatcgcata acgatttagg cattatccgt tacatggtcg
     2881 ccatgggaac ggcacattat gtcgtcatga tcgaccccgc ttcctttatt gatgtcattc
     2941 cctatagctc atggcaaatt gatgccgcca ttattggcaa tgcccataac gttgtcataa
     3001 ccagcagcga tgaaattgct cagggaatta ttaccaggct acaaaaaaca cccggtgagc
     3061 atatcgaaaa taatggaatc atttacgata tcctgccctt cccggagatg aatatttcga
     3121 tcatcacctg ggcttcaacg aaaatgttgc agaaaggctg gcatcggcaa gtctttattt
     3181 ggttaccgct cgggttggtg attggcctgc tggcagcgat gtttgtgctg cgtattttgc
     3241 gccgtatcca gtcaccgcat catcggctgc aggatgctat cgaaaatcgt gatatttgcg
     3301 tgcactatca gccgattgtc tccttagcca atggcaaaat tgtcggtgct gaggcactgg
     3361 cgcgctggcc gcagacagac ggtagttggt tgtcaccaga tagttttatt ccgctggcac
     3421 agcaaacggg cctttctgag ccattgacgc tactgattat aagaagcgtc tttgaagata
     3481 tgggcgactg gctgcgtcag catccacagc agcatatttc gatcaatctt gaatccaccg
     3541 tgctcacctc ggaaaaaatc ccgcaattgc tgcgtgaaat gatcaatcac tatcaggtta
     3601 atcccagaca gatcgcgctt gaactcactg aacgcgagtt tgccgatccg aaaaccagcg
     3661 ccccgataat ttctcgctac cgggaggcgg gccatgaaat ttatcttgat gattttggta
     3721 cgggatattc aagtttaagt tatttacagg atctggatgt cgacattctg aagatcgata
     3781 aatctttcgt tgatgcgctg gaatataaaa atgtcacgcc gcatatcatc gaaatggcaa
     3841 aaacactgaa actgaaaatg gtagcggagg gaatcgaaac cagtaaacaa gaagagtggc
     3901 tacgccagca tggcgtgcac tacggtcagg gctggctcta cagcaaggca ttaccgaaag
     3961 aagatttctt acgctgggcc gagcaacatt tgtgaatttt tgggctgacg gcacgccgca
     4021 cagcccgtca cgctacctat tcgtcgatga ttgactcgaa tccgccatag atcattcgct
     4081 tgccgtcaaa cggcatggac tcgccgaact ctttcatccg tgggtccgac atcatctttt
     4141 gagtagcagc gtcgcggacc tcttttgaag ggtattcaat ccagctaaag acaacctctt
     4201 cattctcttc cgctttcacc gccatacgaa aatcggtcac tttgccatcc ggtacatcgc
     4261 tggcccagca ttcgacaata cgaagcgcgc caaactcttt aaacaatggc gcagccttag
     4321 cggccatttc ccgataggca tcctttttat cggcaggaac ggcaaccaca aaaccatcaa
     4381 catacttcat cagacatacc tccgaagcgt actgcgcagc cagacaattc ttgcgacgtc
     4441 agttgtggtt aaagtttagc catgttgggg ttttttgacg aaacggtggg aggcagaaat
     4501 gggtgggggc cctgccatca gccattagta ctaaattgtg ttggttaatc aattggttat
     4561 agtggtagta ctacaacagt actgtaccac tctagatagt accgtccagc acaaaatctt
     4621 gcaaggtaac gtgccgtcat ggttggggac atgacatgca cttttcgaaa tttatagtgt
     4681 cggaagtgcc tacccgatgt acataaaaaa ggggcgacaa gccccttatt caattacttc
     4741 ttatcctttg gtggacaagg atcgttacca tgcgaatctt tttttgcgat cctgccatcc
     4801 ttgttatgga tcacaagctc acttttttgg tttgtagcaa tccctcttgc agcgtccacc
     4861 gcagattgct ttgttgagtg aacggaggtc gctttactgt ttcctgcccc cttaacggcc
     4921 caacctccat catgaggaac aacatgctga tctttaccca ttatcgaacc ctctatttcc
     4981 atggaaacat cagcatatag cgacaaaacc aaccactcaa taccacatat tgtgtttaaa
     5041 ttgattttaa tcacaaaacg tggatagata tcgaaaggat tctttcacta cgaaactgta
     5101 agtacccttc cttaacccta taccgctata ttcgttaaca atctggcagt gctggcctac
     5161 tctgcccttt gttacccact attctgctgt aagagatgag tttttagcca cctttgcgat
     5221 agtagccctt gaacaaccga tcaaagcctg aattttgctc cagcttttac cgtcagacag
     5281 taattcttgt actttatgat gcaggctctc atcaactggg cgaccacgat attttttctc
     5341 tctcttggcc ttttcaatac cctgcttttg tcgtctgcgg cgatcttcgt agtccttgcg
     5401 actgattgcc gccagcatat cgagcatcat agaattcatt gcttcaaaca tacgaacggt
     5461 gaactcatcc tgaacatgta aaagctgatg gctcgtaggc aaatctaacg atacaaccct
     5521 gatcccttta gattcgatga gtcctcgtaa ggttttccag tcctctgcgg taagtcggct
     5581 tatacgatct atttgctcac agagcagaat atcgccacgt tccgcgatat ctaagagtaa
     5641 aaataattga ggccgctgta ggcttgcacc tgattcattt tctttaaagt actttgatat
     5701 tttcagatca tgcgaatgag caaatgcctc caactctttc aatgccctat ccgcatcttg
     5761 ctctttcgtc gaagctctta gatatgccca tacacgcatg atagtcacca tcagtctgtt
     5821 ttatatggtt cattatatat ggtctgtttt aggttgtaaa atccatttaa tgaatcattt
     5881 tgaattagat atgtgtaggt ctgctagagg tatacatatg atgcactagg aatactgcac
     5941 atcaataaca ataagttact atttccaatg aaataatatc catttacgca tagacaaatc
     6001 gagctaactg tctggcttat agagaatttt tcatgtttaa ttagatgtat cgcagtcggc
     6061 agtgtttatg ttagtgacaa cctcttctaa ttttttgtaa aggtgctggt agatagattc
     6121 attcggaata tactgtatga ataaccagtg ctgtgatttg atttcttata aggacttatc
     6181 agcaatgagt gaacttaaag ttatatcaca cgcaatgttt aacatatccg tagaacaggc
     6241 agaagcaagt agggtagacc ttgaacacgg tgagggcgat tttcagaagt attgcggcca
     6301 attgttagat gagttactgg aaaatactcg cagcaaatcc tttaaattca gagcaataga
     6361 agaatttgtc cctgctcatc ttaacgtgct tgttaatgat caaaacgagt gggacaagag
     6421 aacacttgga attgctgaaa aacttttgtc cgtggaaata gatgctcaag ataaaattaa
     6481 ggctatgaag aaaaaaatta aaaaaggagc cttattaatt ttactacttt ctcgtgacaa
     6541 taatttcaat ttcgtaatac ttaaaattga acatagcgat ttttttgatg agatagagtc
     6601 aaaaatcaaa aaaggacttc ctcttaatcg tcaaaggctc caaaaatcat gtttagttag
     6661 cttcagcaac acatatgatg ttgaagagat tcttatatcg gatagcggag cttcaattag
     6721 tgaatattgg tggaaaaact tcctcagtac tgtagaactt caaagttctg aacttaatac
     6781 aaaaaatgcg tttggttcaa ttgaaaactt tcttaaaagg gaagtagaaa agcactcttc
     6841 cgtggattat tggtatatgc gtaatgacat tattgcttac tttagaaaca atgagcaatt
     6901 tgcatacgat gaattagtag agaaagtacg ttcacataag ccagaaagcc cagctattga
     6961 tgaacgtttc gaccaaataa tcgagaaatt taaagcatta ccagatcata aaaactcgaa
     7021 atttgatagg caatttcaat tagaaccaaa tgtaattcgt gctagaataa agaaaacaat
     7081 agctttgtca gacaacattg aattaagaat caacggggaa gttgctgatt tccgtaaaaa
     7141 aatagttccc gaatcagatg cgttaggtaa atatctgaaa atctactcag atactggtta
     7201 cgaagaattt agtgcaggaa aagaagataa cgtatgaata tagtatctaa aatagccgat
     7261 ttattcatgc catcgcaaga agtttcttct tgcgacaact ttcatttatt taaggctgaa
     7321 tatcgtattt tagaaactac tatccatcaa gattattctc aacgggtgga agaattaaca
     7381 aatgaagtca tagctaacaa tctagatatt gaaattgact ttaagtttga gacttgtgat
     7441 tcattcaccc ttcttatttc cccagaatat t
//
LOCUS       DAPHDX010000177         7385 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPHDX010000177 DAPHDX010000000
VERSION     DAPHDX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.179"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..382
                     /locus_tag="RH058_004030"
     CDS             <1..382
                     /locus_tag="RH058_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139540.1"
                     /translation="TFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALS
                     DHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTD
                     NPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(351..2087)
                     /gene="aceK"
                     /locus_tag="RH058_004031"
     CDS             complement(351..2087)
                     /gene="aceK"
                     /locus_tag="RH058_004031"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV3139541.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2368..3672)
                     /gene="aceA"
                     /locus_tag="RH058_004032"
     CDS             complement(2368..3672)
                     /gene="aceA"
                     /locus_tag="RH058_004032"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV3139542.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPELELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTAMTGSTEESQF"
     gene            complement(3702..5303)
                     /gene="aceB"
                     /locus_tag="RH058_004033"
     CDS             complement(3702..5303)
                     /gene="aceB"
                     /locus_tag="RH058_004033"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV3139543.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(5572..6501)
                     /gene="metA"
                     /locus_tag="RH058_004034"
     CDS             complement(5572..6501)
                     /gene="metA"
                     /locus_tag="RH058_004034"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV3139544.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6658..7101
                     /locus_tag="RH058_004035"
     CDS             6658..7101
                     /locus_tag="RH058_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV3139545.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            complement(7219..7334)
                     /gene="rrf"
                     /locus_tag="RH058_004036"
     rRNA            complement(7219..7334)
                     /gene="rrf"
                     /locus_tag="RH058_004036"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1774 a         1903 c         1902 g         1806 t
ORIGIN      
        1 tacgtttaaa ttcgataaaa aaaatatgaa gcccctcctc ctggcaaata attctaatga
       61 atatcccggt ttgttttcag cgatacagca taaacaacaa aacgttgtag agacggttta
      121 tcttgcttta tctgaccatg cacgcctgtt tggatttacc gctgaagata ttatggattt
      181 ttggcaacac aaagccccac aaaaatactc tgcctttgag ttggcttttg aattgggtca
      241 ccgggttatt gctgaattaa tccttaatac attaaataag atggctgaaa gctttggctt
      301 tacggataac cctcgataca ttgcggagaa aaattatatg gaagctttac tcaaaaaagc
      361 atctccccat accgtacgct aaatctttgc ctgcgccgat acgcataaac atcttccaca
      421 tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caggtcggcg
      481 tgcatctctt caaacagcgg accaatacgc gggtcggcgc atagccagtg gcgaaactct
      541 tccgggaaaa catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcttcc
      601 ggatagcgcg gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca aatttcatcg
      661 taatcataaa aaaccacacg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg
      721 ccaggaaaaa tgttagcagc ggcaagctgg cgaatagcgt taccgtattc ttcaatggcg
      781 tcgcgcaact gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc
      841 tcgatataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt ttccgctgct
      901 tcctggagca gtaattccat taatgccggg gaaatatgcc gcttctccag cacaaagttt
      961 tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac cagttgatag
     1021 caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc tttgatgact
     1081 ttgaataccc gatcaaagcc cggcagcgta aataccaaca tcaccattcc acgaataccc
     1141 ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc gcggtagctt
     1201 tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt ggttttacct
     1261 ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata aaccataaaa
     1321 taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag gcaggtatca
     1381 ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa cgtgccggaa
     1441 ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc
     1501 acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag atggcgaatg
     1561 atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag gtcgctgata
     1621 acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg
     1681 ggaatggtac gaaagcggcg ctctggctga gaactaaaga taaaaagccg ctcgggagta
     1741 agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc cgcaatctcg
     1801 aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag taaaaatgcc
     1861 gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag accaacgtga
     1921 tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca gtcggcctgt
     1981 tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg agcgtcgaag
     2041 ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta gcgccctcat
     2101 caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg ttcggcaacg
     2161 gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat
     2221 gcggcgtaaa tgccttatcc ggcctacgtt cggcaatggc tgtaggcatg ataagacgcg
     2281 caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc ggcgtgaacg ccttatccgg
     2341 cctacagtca gcaacggttg ttgttgctta gaactgcgat tcttcagtgg agccggtcat
     2401 cgcggtgact gaagacgtgc cgccctgaat aatagtcgtc actttatcga agtaacctgt
     2461 acccacttcc tgctggtgag atacgaaggt atagccatct ttcgcggcgg caaattccgg
     2521 ctgctgcact ttctcaacgt agtgcttcat accctcgccc tgggcatagg cgtttgccag
     2581 gtcaaacatg ttgaaccaca tgctgtggat acctgccagg gtgatgaact ggaacttgta
     2641 gcccatatcc gacagctgct gctggaagct ggcaatagtt ttgtcgtcga ggtttttctg
     2701 ccagttgaac gacggcgagc agttataagc cagcagtttg cccggatatt tcgcgtggat
     2761 agcttgtgca aagcgacgcg ccagttccag ctccggcgtg gaggtttcac accagaccag
     2821 gtcagcatat ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc ccgcatgagt
     2881 acggaagaag ccttcactgg tacgctcgcc agtaataaat tcactgtcat acgggtcgca
     2941 atcggaggta atcagatccg ccgcatcggc atcggtacga gcaaccagca gggttggaac
     3001 gcccgtcacg tcagctgcaa gacgcgccgc gaccagtttc tgaatagctt cctgagttgg
     3061 caccaaaact ttgccgccca tgtgaccgca tttcttcact gacgccagct gatcttcgaa
     3121 gtgaactgcc gctgcaccgg cttcaatcat cgctttcatc agttcaaagg cattcaggac
     3181 accgccaaaa ccggcttccg catcggcaac gatcggcagg aagtaatcga catagcgcgg
     3241 atcgcccggc tcaatgcctg cggaccattg gatctgatcg gcacgacgga aggtgttgtt
     3301 gatccgctcc accacagctg gcaccgagtt tgccggatag agcgactgat ccggatacat
     3361 gctggccgcc aggttagcgt ccgccgctac ctgccatccc gacagataga ctgcttcaat
     3421 acccgctttc gcctgttgca gcgcctgacc gccagtcagt gcaccgaggc tgttgatgta
     3481 gccttttttc gactcgccat gcagcagacg ccacattttc gccgcgccca gttgcgccag
     3541 ggtgcattca ggattgactg aaccgcgtaa tttcaccaca tcttccgcgc tgtatgggcg
     3601 agtaatgcct tcccaacgcg gttgagtcca ctctttctgt aattcttcaa tttgttgtgt
     3661 acgggttttc atgtgcagat gctccatagt tatgtggtgg tttacgctaa caggcggtag
     3721 cctggcaggg tcaggaaatc aattaactca tcggaagtgg tgatctgttc catcaagcgt
     3781 gcggcatcgt caaaacgccc ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact
     3841 ttcatctctt cacccagcat ctggcggaac aaggctttgg tcaccggttt gccattgctc
     3901 aacgtttttt gatgatggat ccactgccag attgaggtac gggaaatttc agccgtcgcc
     3961 gcatcttcca tcaggccata aatcggcacg cagccgttgc cggagatcca tgcttcgatg
     4021 tactgcactg ccacgcgaat gttggcgcgc ataccttctt cggtgcgttc accatcacaa
     4081 ggtgccagca gctgatcggc agtaatcggc acgtcttgtt cgcgcatcac ttcaagctga
     4141 tttttacggg agccgagaat gtcgttgaat accgccattg ccgtatccgc aaggcctggg
     4201 tgagcaatcc atgtgccatc gtgaccgtta ttggcttcca gcgctttatc cgcttttact
     4261 ttgttgagca cctggttatt gcgctcttca tctttgctcg gaataaacgc tgccatgccg
     4321 cccatcgcaa aagcaccgcg tttatggcag gttttaatca acaggcgtga gtaagcattc
     4381 aggaatggtt tatccatcgt cactgcctgt ctgtctggca ggacgcgatc gggatagttt
     4441 ttcaacgttt tgatatagct gaagatgtaa tcccaacgac cgcagttcag accaacaata
     4501 tggtcacgca gcgcgtgaag gatttcatcc atctggaaca cggcgggcag cgtttcaatc
     4561 agcaacgtcg ccttgatggt gccgcgcggc agattaaagc gatcttctgc atagctgaag
     4621 acttcgctcc accaggccgc ttcctgccag gactgggttt tcggcagata gaaataggga
     4681 ccgctgccct ttgccaacag tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac
     4741 aggctaccgg ggattgcctc accacgccag gtgacatgtt tttccggcaa gtgcagaccg
     4801 cgtacccgac aaatcaaaac cgctggattg ggcttgagct gataaatttt gcctgcttca
     4861 ttggtatagc tgatggtgcc gttaaccgca tcgcgcaggt taatttgccc gtcgatcact
     4921 ttgttccagt ctggtgccag tgaatcttcg aaatcggcca taaagacttt cacattggcg
     4981 ttcagtgcgt tgatcaccat cttgcgctca accgggccag ttatctcgac tcgacgatct
     5041 tgtaagtccg cgggaatacc acgaattttc cagtccgtat cgcgaatgga agctgtttcc
     5101 gaaataaaat caggcaaagt tccgttatca atatcttgct gctgctgaat gcgcgctgcc
     5161 agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct
     5221 tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc ttatgaaagc cagttcatcg
     5281 gttgttgttg cctgttcagt catcgtgcag ctcctcgtca tggatccgaa aacttcccca
     5341 ctgtgaacga aggatcgttg tgcgcttttc gttcagcaca actaagacta ctcatttaaa
     5401 atgcaaaatc aaaaacaatt tccattttaa ttaaaaacac ttgataactt attgataaca
     5461 atatgaataa aatttattca cctcctgagg tgcgtttcgg aaataaaaaa ggcacccgaa
     5521 ggtgcctgag gtaaggtgct gaatcgctta acgatcgact atcacagaag attaatccag
     5581 cgttggattc atgtgccgta gatcgtatgg cgtgatctga tagacgtaat agttgagcca
     5641 gttggtaaac agcaaattac cgtgactacg ccagctcgct cgcggtgtat tttgcggatc
     5701 attgtgcggg aaatagttat acggtacttc cgggcctagt ccggcttcca catcgcggaa
     5761 atattcctgc gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg
     5821 cttatctttg ctggcaaaca gatatgcatc cccttcttcc gtctctgcca gaatttccag
     5881 atcggtgtaa tcacgaatca acgctgccgg aaagtcagca tagcgcgaat gtggtgccag
     5941 gaatgaatca tcaaagccac gcgtcagaag cgcatgagga tggagaatat gatgctcgta
     6001 aacgccagag agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga tattgagcgc
     6061 ggcctgtacc gcccagcaga caaacagcgt cgaggtgacg tgatctttcg accactccag
     6121 cacctgtttg atctgcggcc agtaagcgac atcattaaac tccaccaggc ccagcggcgc
     6181 accggttaca atcagaccgt caaagttctg ttcctgaata tcttcaaagt tacagtagaa
     6241 gttgttcaga tgctctgcgg gcgtgttgcg cgattcacgg gaatcgatgc gcaacagctg
     6301 aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga aactgatttt cagtttcaat
     6361 cttcttcggc atcaggttaa ggatcagaac cttaagtgga cgaatttcct gaccagacgc
     6421 acgagaagtt gtcatcacaa agacgttttc ttcacgcaag aaattgacgg cgggtagctc
     6481 gtccggcaca cgaataggca taacctgatt acctcactac atacgcttat acgtttagac
     6541 atccagatag ctgaagataa ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg
     6601 agaaagtttc aaggaaaagg ctacgttaga atataagaat gacgaaaagg agagaaaatg
     6661 gttattagta ttcgccgctc acagcatgag gaaggggagg aactcgttgc gatttggtgt
     6721 cgatctgtcg atgccaccca cgatttttta tcagcagagt atcgggccga gctggaagag
     6781 ctggttcgtt cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggatcag
     6841 ccggttgggt ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat
     6901 gtgcgcggct gcggcgtggg tcggatgctg gtggagcatg cgctctcgat ggcaccggaa
     6961 ctgacaacca acgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt
     7021 tttaaggtta cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctgaat
     7081 ctggcgtatg tgggggaata agccgcccct gccgtgcctg cccaggctgg agaagacgcg
     7141 ccagcgtcgc atccgacctt tttctgcacc agcgcaaaaa ggcccagtct tccgactggg
     7201 ccttctgctt aatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
     7261 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
     7321 ctaaggccgc caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt
     7381 atcag
//
LOCUS       DAPHDX010000178         7304 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPHDX010000178 DAPHDX010000000
VERSION     DAPHDX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.180"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..71
                     /locus_tag="RH058_004037"
     CDS             <1..71
                     /locus_tag="RH058_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="DNA-damage-inducible protein J; antitoxin of
                     YafQ-DinJ toxin-antitoxin system; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin,
                     RelB/DinJ family"
                     /protein_id="HDV3139546.1"
                     /translation="SEAGVDVHKAKDADDLFDKLGI"
     gene            74..352
                     /gene="yafQ"
                     /locus_tag="RH058_004038"
     CDS             74..352
                     /gene="yafQ"
                     /locus_tag="RH058_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDV3139547.1"
                     /translation="MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            508..1248
                     /locus_tag="RH058_004039"
     CDS             508..1248
                     /locus_tag="RH058_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV3139548.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1219..1986)
                     /locus_tag="RH058_004040"
     CDS             complement(1219..1986)
                     /locus_tag="RH058_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV3139549.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2192..2770)
                     /gene="lpcA"
                     /locus_tag="RH058_004041"
     CDS             complement(2192..2770)
                     /gene="lpcA"
                     /locus_tag="RH058_004041"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV3139550.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3010..5454
                     /gene="fadE"
                     /locus_tag="RH058_004042"
     CDS             3010..5454
                     /gene="fadE"
                     /locus_tag="RH058_004042"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV3139551.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLAGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5497..5943)
                     /gene="ivy"
                     /locus_tag="RH058_004043"
     CDS             complement(5497..5943)
                     /gene="ivy"
                     /locus_tag="RH058_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV3139552.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6124..6894
                     /gene="yafV"
                     /locus_tag="RH058_004044"
     CDS             6124..6894
                     /gene="yafV"
                     /locus_tag="RH058_004044"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV3139553.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYYLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(6936..>7304)
                     /locus_tag="RH058_004045"
     CDS             complement(6936..>7304)
                     /locus_tag="RH058_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV3139554.1"
                     /translation="LKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAESGLS"
BASE COUNT         1709 a         1837 c         1950 g         1808 t
ORIGIN      
        1 acagcgaagc tggcgttgat gttcataagg ccaaagacgc cgatgattta tttgataaat
       61 taggaattta aatatgattc aaagggatat tgaatactcg ggacaatttt caaaggatgt
      121 aaaagttgca caaaagcgtc ataaggatat gaataaattg aaatatctta tgacgcttct
      181 tatcaataat actttaccgc ttccagctgt ttataaagac cacccgctgc aaggttcatg
      241 gaaaggttat cgcgatgctc atgtcgaacc ggactggatc ctgatttaca aacttaccga
      301 taaactttta cgatttgaga gaactggaac tcacgcggcg ctctttgggt aaagcacgct
      361 gccgatgaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat
      421 gtgagccttc caaatccaga ctattccgat ttgggatcta ttttgccgtc ctggcgtgtg
      481 taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg
      541 ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg
      601 agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa
      661 gaacgtacgc tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat
      721 aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc
      781 ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa
      841 gcgattaata ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac
      901 ctgatgatcc acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt
      961 gatgagatct tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg
     1021 agtatttacc cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt
     1081 aaggacttct gggagcaact gaagccgggt tacgactact ttgagcaaac ccgtaagcca
     1141 ccgaccgttt ctgtggtcaa tggtcgttac gtggtcagca agccgttaag ccacgaagtt
     1201 gtgcagccac aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc
     1261 tggcataatc ttttgccagg tttcattgcc cgtcagcggc tgtgttgcaa tcaccgtgac
     1321 cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat ccagcagcgt
     1381 tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc aatacgccat
     1441 cacatagcgc ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca gttcatccgc
     1501 cagtgaggcg atatatttaa acactgccgc catgttgccc ggtgtgcgcg ggtaacgctg
     1561 cgttaattta tgcaggagcc agcagaaggc tttttcgcta tcggtttcac cgaccgggcg
     1621 gaagttgccg gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca
     1681 gttacgcccc cataactcgc gggtaaatgg gtgagtattt tccagcgcca cctcgccccg
     1741 attagcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag
     1801 tttggcgatg ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc
     1861 ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgtac
     1921 aagtccggtg aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc
     1981 gcacatgggt aacctccaca acagcgggag cgcatggccc ccggcgaaga ttgatttaag
     2041 aattttcagg tcggatgcgg cgtaaacgaa ttattcggcc tacggttcgg gtacagattt
     2101 gtaggcatga taagacgcgt cagcatcgca tcaggcatca gcgcacaagt gccggatgcg
     2161 gcgtaaacgc cttatccggc ctacgccaga cttacttaac catctctttt tcaattaact
     2221 ggatcaggat atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt
     2281 gcggtacgcg aatttcgata tccgccgtgc cagccatttt gccgccgtct ttaccggtca
     2341 gggtgatcac tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag
     2401 agttaccgga ggtggagatc cccagcagta catcgccttc gcgacctacc gcttcaacgt
     2461 agcgggagaa aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt
     2521 cagaaatagc aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt
     2581 cggcaaagtg catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac
     2641 cggctttaaa gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt
     2701 catcttttaa aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat
     2761 cctggtacat gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat
     2821 accgccaaaa gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc
     2881 ggataaaaac agcaacaatg tgagctttgt tgtaattata ttgtaaacac attgctaaat
     2941 gtttttacat ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg
     3001 cttttcgtta tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc
     3061 tatcaccgcg tgagcttatt tatcagcagt ctgattttgc tcgcctggac agccgccctc
     3121 ggcgttgctg gtctgtggtc ggcgtgggta ctggtgcctc tggccattat cctcgtgcca
     3181 tttaactttg cgcctatgcg taagtcgatg atttccgcgc cggtatttcg cggtttccgt
     3241 aaggtgatgc cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg
     3301 tgggagggcg acttgttcca gggcaagccg gactggaaaa agctgcataa ctatccgcag
     3361 ccgcgcctga ccgccgaaga gcaagcgttt ctcgccggcc cggtagaaga agcctgccgg
     3421 atggcgaatg atttccagat tactcatgag ctggcggatc tgccgccgga gttgtgggcg
     3481 taccttaaag agcatcgctt cttcgcgatg attatcaaaa aagagtacgg cggactggag
     3541 ttctcagctt atgcccagtc tcgcgtgctg caaaaactct ccggcgtgag cgggatcctg
     3601 gctattaccg tcggcgtgcc gaactcatta ggcccgggcg agctgctgca acattacggc
     3661 actgacgagc agaaagatca ctatctgccg cgtctggcgc gtggtcagga gatcccttgc
     3721 tttgcactga caagcccgga agcgggttcc gatgcgggcg cgattccgga caccgggatt
     3781 gtctgcatgg gcgaatggca gggccagcag gtgctgggga tgcgtctgac ctggaacaaa
     3841 cgctatatta cgctggcacc gattgcgact gtgcttgggc tggcgtttaa actctccgac
     3901 ccggaaaagc tgctgggtgg cgcagaagat ttaggtatta cctgtgcgct gatcccgacc
     3961 accacgccgg gcgtggaaat tggtcgtcgc cacttcccgc tgaacgtacc gttccagaac
     4021 ggaccgacgc gcggtaaaga tgtcttcgta ccgatcgatt acatcatcgg ggggccgaaa
     4081 atggccgggc aaggctggcg gatgctggtg gagtgcctct cggtaggccg cggcatcacc
     4141 ctgccttcca actcaaccgg cggcgtgaaa tcggtagcgc tggcaacggg cgcgtatgct
     4201 cacattcgcc gtcagttcaa aatctctatc ggtaagatgg aagggattga agagccgctg
     4261 gcgcgtattg ccggtaatgc ctacgtgatg gatgctgcgg catcgctgat tacctacggc
     4321 attatgctcg gcgaaaaacc tgccgtgctg tcggctatcg ttaagtatca ctgtacccac
     4381 cgcgggcagc agtcgattat tgatgcgatg gatatcactg gtggcaaagg cattatgctc
     4441 gggcaaagca acttcctggc gcgtgcttac cagggcgcac cgattgccat caccgttgaa
     4501 ggggctaaca ttctgacccg cagcatgatg atcttcggac aaggagcgat tcgttgccat
     4561 ccgtacgtgc tggaagagat ggaagcggcg aagaacaatg acgtcaacgc gttcgataaa
     4621 ctgttgttca aacatatcgg tcacgtcggt agcaacaaag ttcgcagctt ctggctgggc
     4681 ctgacgcgcg gtttaaccag cagcacgcca accggcgatg ccactaaacg ctactatcag
     4741 cacctgaacc gcctgagcgc caacctcgcc ctgctttctg atgtctcgat ggcagtgctg
     4801 ggcggcagcc tgaaacgtcg cgagcgcatc tcggcccgtc tgggggatat tttaagccag
     4861 ctctacctcg cctctgccgt gctgaagcgt tatgacgacg aaggccgtaa tgaagccgac
     4921 ctgccgctgg tgcactgggg cgtacaagat gcgctgtatc aggctgaaca ggcgatggat
     4981 gatttactgc aaaacttccc gaaccgcgtg gttgccgggc tgctgaatgt ggtgatcttc
     5041 ccgaccggac gtcattatct ggcaccttct gacaagctgg atcataaagt ggcgaagatt
     5101 ttacaagtgc cgaacgccac ccgttcccgc attggtcgcg gtcagtacct gacgccgagc
     5161 gagcataatc cggttggctt gctggaagag gcgctggtgg atgtgattgc cgccgaccca
     5221 attcatcagc ggatctgtaa agagctgggt aaaaacctgc cgtttacccg tctggatgaa
     5281 ctggcgcaca acgcgctggc gaaggggctg attgataaag atgaagccgc tattctggtg
     5341 aaagctgaag aaagccgtct gcgcagtatt aacgttgatg actttgatcc ggaagagctg
     5401 gcgacgaagc cggtaaagtt gccggagaaa gtgcggaaag ttgaagccgc gtaaatgaat
     5461 aacggagccg aaaggctccg tttctttatc cgctaattat ttaaaattaa agccatccgg
     5521 atggttttcc aggctgccgg tcaacgccgc gaacaacacc gttttaccat caatcgaaag
     5581 cgcatcgttc acattcagcc aggtgagttt ctcttgcgac gttttctcat caatagtcga
     5641 gaacagcccc gtcatctgat tagatttctc ggaccacatc acagcgatac gttgcgagcc
     5701 acagtcatgc ggtttgcacg cgctcatcac ctgatacgtc tcatctccca acgttacggt
     5761 ttgtgcggga gtataagtac cgcctttcat cacccaggca ggcagcttat gcccttgtac
     5821 catctgatta aatgcagctt tggtggtttc gccctttgca aggctgctaa tggttaaatc
     5881 atcctgcgcc attgcactgg tggcgatgac cagagcggcg actgtcgtta ttgccttaaa
     5941 catcattcct cccgagctta tcctgctcat gttattaacc ttctaaagcc tggcgataac
     6001 acgataattt gcaaatcaga tatttccaat atcagcattt gttatcaatt tattctgcgc
     6061 taaggcaact gcctgataag gtgttaaagt tataaaaccc ttcgcataaa ggagttgtac
     6121 accgtgcctg gtttgaagat tacgcttttg cagcaaccac tggtgtggat ggatggccct
     6181 gccaacctgc gtcattttga tcgtcaactg gaaggtatta ccgggcgcga tgtgatcgtt
     6241 ctaccggaga tgtttaccag cggctttgcc atggaagcgg cagcttcgtc gctagcacaa
     6301 gatgacgtag tgaactggat gacagctaag gcgcagcagt gcaatgcgct gattgcaggc
     6361 agtgttgcat tacaaacgga gtctggttcg gttaaccgct ttttgctggt tgagccgggc
     6421 ggcacggtac atttttatga taagcgtcat ctgttccgca tggcagatga gcatctacat
     6481 tataaagcgg gcaatgcgcg agtgattgtg gaatggcgcg gctggcgtat tttgccgttg
     6541 gtgtgctacg acttacgttt tccggtgtgg tcgcgcaatc tcaacgatta ttacctcgcc
     6601 atctacgttg ccaactggcc tgctccgcgc tctctgcact ggcaggcatt gctgacggcc
     6661 cgcgcgattg agaatcaggc gtatgtggcg ggatgcaatc gcgtcggcag cgatggcaac
     6721 ggctgccatt atcgcggtga cagccgggtg attaacccgc agggcgagat tatcgctact
     6781 gccgacgcgc atcaggcaac gcgcattgat gcggagctgt cgatggtggc attgcgggaa
     6841 tatagagaaa agtttccggc atggcaggat gcggatgagt ttaggttgcg gtgagtgggg
     6901 gttgtgttta aggctggggg agagtcgggg taagactacg aaagcccgct ctccgcaagg
     6961 actgacgcca gatagtttct gtccatagct gcttttcgca tcttacgtct taaccctgcc
     7021 ttgaatacct tatcattcgt caaaatatta atagcgatgt gccgtatccc tgaaaataat
     7081 tctgcggcgt ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc
     7141 cagtgcagct tattctccac gtgccagtgg tttcggattg ctgtggcgaa cttttctgcg
     7201 gttaaatcag cagaactgat ataatatctg accgtcattt ctggctcttt cttttgttct
     7261 gctattattg accgaaagga gactgccacg cataatttct tcag
//
LOCUS       DAPHDX010000179         7212 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPHDX010000179 DAPHDX010000000
VERSION     DAPHDX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.182"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            66..251
                     /locus_tag="RH058_004046"
     CDS             66..251
                     /locus_tag="RH058_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139555.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            212..331
                     /locus_tag="RH058_004047"
     CDS             212..331
                     /locus_tag="RH058_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV3139556.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            366..710
                     /locus_tag="RH058_004048"
                     /pseudo
     CDS             366..710
                     /locus_tag="RH058_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1082..1348)
                     /gene="minE"
                     /locus_tag="RH058_004049"
     CDS             complement(1082..1348)
                     /gene="minE"
                     /locus_tag="RH058_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV3139557.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1352..2164)
                     /gene="minD"
                     /locus_tag="RH058_004050"
     CDS             complement(1352..2164)
                     /gene="minD"
                     /locus_tag="RH058_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV3139558.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2188..2883)
                     /gene="minC"
                     /locus_tag="RH058_004051"
     CDS             complement(2188..2883)
                     /gene="minC"
                     /locus_tag="RH058_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV3139559.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3403..3771
                     /locus_tag="RH058_004052"
     CDS             3403..3771
                     /locus_tag="RH058_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139560.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(3891..4292)
                     /locus_tag="RH058_004053"
     CDS             complement(3891..4292)
                     /locus_tag="RH058_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV3139561.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4533..4826
                     /locus_tag="RH058_004054"
     CDS             4533..4826
                     /locus_tag="RH058_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV3139562.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4898..5557
                     /locus_tag="RH058_004055"
     CDS             4898..5557
                     /locus_tag="RH058_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV3139563.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            5649..6095
                     /locus_tag="RH058_004056"
     CDS             5649..6095
                     /locus_tag="RH058_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV3139564.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6302..7204)
                     /gene="hlyE"
                     /locus_tag="RH058_004057"
     CDS             complement(6302..7204)
                     /gene="hlyE"
                     /locus_tag="RH058_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131199601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDV3139565.1"
                     /translation="MVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKEL
                     SRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYN
                     EKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKS
                     SYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1844 a         1588 c         1711 g         2069 t
ORIGIN      
        1 actgttaacg caatgttgta aaggtaagat aatctgattt atcaatatta ttgtgtgatt
       61 tttatgtgag cagaagatat tcatcagcaa cgattacatt agtcatttta ttttgccgac
      121 ggcctcattg tcgaaagata agcgtacgac agtattatct gaaaagagtg attttttatc
      181 caactacact tcagcgcact gcgtgtaaaa aatgcctctt tcttatgcgg gatatcatca
      241 tttcatcatg atgtctttgg tgagcggtga acacaataca cctgcgctgt ctcttcagga
      301 tgaatcccct ggtctggtgc ctgcgggctg atgttgcagc agagcttagg tcacttagac
      361 gctactatca tttatccaat ggcatggaat cgaaatcagt cgatacccgc agtatatatc
      421 gtgaactggg tgcaacgctg agttacaaca tgcgtctggg gaacggtatg gaaattgaac
      481 cctgcctgaa ggcggctgtg cgcaaagaat ttgtcgatga taatcgggtg aaagtgaata
      541 gtgacggtaa ttttgtcaat gatttgtcgg gcagacgtgg aatataccag gcaggtatta
      601 aagcctcatt cagcagtacg ttaagcgggc atttcggggt ggggtatagc catggtgccg
      661 gtgtggagtc cccgtggaac gcggtggctg gtgtgaactg gtcgttctga ccatcaacga
      721 ttaaactgcg cttcggcgca gttttcgttt acaggatgtt gaaagagaaa attctggggc
      781 aaaaaaagcc cgccagttac ggcgggaaac ctcatcctat gggagaacaa tgaataatga
      841 aattgcgggg ttatcatctc ccagtatatc cacactaaca acatggttat ttactcaacc
      901 aggcataaac attttgttct gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga
      961 aatagcaatg aggagtatca gcaagaatac tcgccgcttt accacaacct ggatgagagg
     1021 gatgaaaaac tcaaggcaga aatgactctg ccttgaagat aaatgcgttt ttacagcggg
     1081 cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc
     1141 catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa
     1201 tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac
     1261 ggcgttcagc aacaataatc tgcagccgtt cttttgcaat gttggctgtg tttttcttcc
     1321 gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc
     1381 ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg
     1441 tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt
     1501 tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga
     1561 tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc
     1621 gcgttaacag caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgatttcg
     1681 acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg
     1741 taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg
     1801 gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga
     1861 ccccttcacg ggtgagggca tctttatcgc gtgtttgcga tgccggaaga atatagagat
     1921 tttcagtacg cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt
     1981 tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga ttacgcaggc
     2041 cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg
     2101 ccgcgctgga ggttgtctta ccaacacccc ctttgcccga agtaacaaca ataatgcgtg
     2161 ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc
     2221 gactaactgc agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca
     2281 gtattcacct gcgatggaca ccagttccgc catcaggttc gtacaaaata tttgcgtttc
     2341 ccggtcacca cttgcccctg caagcgcacg accgcgcatc atgccataga catgaatgtt
     2401 cccatcggca atcaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg
     2461 tggagcataa atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac
     2521 cggcgttgta ttttgcgctg gagcctgtgg tgcgggagct ggacgcggcg ctttttcctt
     2581 tccttccgtc aggataggca gccccatctt ttcaatttcg gctttaagtt gcgcatcttt
     2641 gcagccactt acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga
     2701 ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa
     2761 tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc
     2821 atgcagatga accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga
     2881 catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga
     2941 ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga
     3001 gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa
     3061 tcaacccatt gccgtcgtac ttttgattgt tcttatttac gcttcctttt tccgcaccct
     3121 aatcgtgaca gcatctccag acctgtttaa atatccagta ttgaggcaag tgtatttttt
     3181 caccacgcca ggctttctta actttccatt ttttgcggca tcattaaaga acagataccc
     3241 gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata
     3301 cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgatga
     3361 aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt
     3421 ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc tggacatgac
     3481 gctggcggac ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat ttgtgtgaac
     3541 tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac
     3601 ttacaatcat tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc
     3661 gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtggatgg taagcggagt
     3721 ggaaaaatca atcaaatcac cacaaagatg ttatttatgt gtcgtgagtg atgccttgtt
     3781 tacattatgt tctattcggc attgtctgct attcgtcatt aacctcttga gcgacagaga
     3841 ggagtggggc gttaacgttt atgacgggga gagtccccgt cggttgacat ttacttaatt
     3901 tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc
     3961 actcgcagct catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct
     4021 ggtgaatatc tggataaatc aactgaatca tcgattcctg gcccgaagag gtaagtatca
     4081 gcgccttcat tagagatact cacatgcact ttctgacctt ttttggcata gaaggtatat
     4141 gtatcgtaat cgtatccctt tatttcgccg gagtactgag cgctgctatg gcctttgctg
     4201 aactcaacat tgacattctt acccgcagca aagctcgtgg aggttagcag tgcaagcagc
     4261 aatacagcct tactgatgct cttgattttc attggtagtc tcctgttcag aatgagtacg
     4321 atgaactgtt ataatataac aatcccttac gggagaattc cagtggcttt tctgcgttcg
     4381 gtaaaatccc ggcgcggcat tttttgccgg ggggattact tataaagggg catggactca
     4441 acaaaccgta gaaatctaca gctggctggc ttgtggcgcg ggttcattga tgttagctta
     4501 tgccaaaacc tggcatctta aagagtaaaa gtatgttttg tgtgatttat cgaagcagca
     4561 agcgtgacca gacctattta tatgtcgaaa aaaaagacga tttttcgcgt gttcctgagg
     4621 aactgatgaa aggttttggt cagcctcagt tagcgatgat tctgccgttg gatgggcgga
     4681 agaaactggt caatgctgat attgaaaagg ttaaactggc attaaccgag caaggttact
     4741 atttgcagtt accgccacca cccgaagatt tgctgaagca acatctttcc gtcatggggc
     4801 agaaaacaga cgacactaac aaataaccga tatccggcgg tggcattatc tttgtcggcg
     4861 cgggttttca tatccacgat aaggtgaggg gaacgttatg tatcaacatc acaactggca
     4921 aggtgcgctg ctggattatc cggtgagtaa agtagtctgt gttggcagta actatgccaa
     4981 acatattaaa gagatgggca gcgcagtgcc cgaagagcca gtgctgttta ttaaaccaga
     5041 aacggcactg tgcgatctgc ggcagccatt ggcgatccca tccgatttcg gttcagttca
     5101 tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg cgtcaggcta cggaagagca
     5161 tgtccgcaaa gccattgccg gttatggcgt ggcgctcgat ctgacattgc gtgatgttca
     5221 gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggct aaagcgtttg ataactcttg
     5281 tccgctttcc gggtttattc ccgcggcgga atttaccggc gacccgcaaa atacaacgct
     5341 gggcctgagc gtaaacggcg agcaacgtca gcaaggtacg actgcggaca tgatccataa
     5401 aattgttccg ctgatcgctt atatgagcaa gttttttacc ctcaaggccg gtgacgttgt
     5461 gctgacaggc acgcctgatg gcgtcggccc gttgcaaagc ggtgatgagc tgacagtcac
     5521 tttcgatggg cattctttgg caactcgcgt tttgtaatac tttttgccgc ctgaaagcgg
     5581 cggcaaaact tgcatcgctg tgccagactg gttataaggt gcgttttaac gtaatggcgg
     5641 aacacctgat gagcgatgta cctttctggc aaagtaaaac cctggacgaa atgagcgatg
     5701 cggaatggga gtcgttgtgt gatggttgcg gtcagtgttg cctgcataaa ctgatggatg
     5761 aagacaccga cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat attaaaacct
     5821 gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt aaattaaccc
     5881 gtgaaaatct gccaacattc gaatggctgc caatgacctg cgcttatcgt ttgctggcgg
     5941 aaggtaaaga tttacctgcg tggcatccgc tacttactgg ttcgaaagcg gcaatgcatg
     6001 gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt gactggcagg
     6061 atcatatctt aaataaacct gactgggcgc agtgataaat ttaatagatg ttgatggggg
     6121 ctttcttaat atacgtctgg atgctcgcca tttcgtagcc ctgtttttga aagcgctctg
     6181 tcataatgag agttacccga taccttatta tcaacaggta actctcaatc tttaaaaaac
     6241 gtctgtgttt ttaaatgtga taaaccttat accttgagta catggagaga ataatcgctt
     6301 atcagacttc aggtacctca aagagtgtct ttttaccgtg tcttttctga tactcattac
     6361 aggtgttaat catttttttg gccgcttctt ttagcaaaga aagcattaaa tcatcataat
     6421 caacatagaa tctggttgtt tcagtttccg tttttatctc cccgatggcg gctatttcgg
     6481 tggttaattt caatttggcg gcatcgatat ctttattcgc ttgtttaacc gtgttagaca
     6541 gggtggtaaa gaaactctgc acagatttta acttgttctt caattctggg atcagtttcc
     6601 cttcaactac gcccgcagca atagaatagg aaatgattaa tccaaatgga ccagcgacga
     6661 caccggctgc ggcaccggca tacgcttcct tcctgatttt atctacctgt gactggaaat
     6721 agctgctttt ttctgaaaaa tcattggtta actggctatc taacgccagc agtttcccgg
     6781 aagcgttgtt gaaactttgt gagcttacca gcagggattt ttgcgcttca ttcagcttcg
     6841 tgatgccgtc atccagtacc ttaatgagaa tgtctttctg ggcggatgct ttcttctcat
     6901 tgtactcatc aaatagcaaa atatacgctg cgagcaattg cgtcgcaaca ccacaccatt
     6961 catacactgt ttgggttgct tcaaaatact tatcctggct atccataagt aaggttttaa
     7021 tatcgccgac taaaacggag gctgcctgtg aatactcctg tttaaagcga cttaactctt
     7081 ttatggtttc atcaaatgtc tgccagggga tgacctgatc gagatattta ttataaagat
     7141 ctaatgctcc atctgcggtt tcgattgcgt ttttaactac ctctaccgtt ttatctgcaa
     7201 cgatttcagt ca
//
LOCUS       DAPHDX010000180         7165 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPHDX010000180 DAPHDX010000000
VERSION     DAPHDX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.183"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(95..730)
                     /gene="msrQ"
                     /locus_tag="RH058_004058"
     CDS             complement(95..730)
                     /gene="msrQ"
                     /locus_tag="RH058_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV3139566.1"
                     /translation="MRLTVKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(731..1735)
                     /gene="msrP"
                     /locus_tag="RH058_004059"
     CDS             complement(731..1735)
                     /gene="msrP"
                     /locus_tag="RH058_004059"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV3139567.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1844..2257)
                     /gene="hiuH"
                     /locus_tag="RH058_004060"
     CDS             complement(1844..2257)
                     /gene="hiuH"
                     /locus_tag="RH058_004060"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV3139568.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2390..3061
                     /gene="hprR"
                     /locus_tag="RH058_004061"
     CDS             2390..3061
                     /gene="hprR"
                     /locus_tag="RH058_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV3139569.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3061..4419
                     /gene="hprS"
                     /locus_tag="RH058_004062"
     CDS             3061..4419
                     /gene="hprS"
                     /locus_tag="RH058_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV3139570.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4527..5378)
                     /gene="hchA"
                     /locus_tag="RH058_004063"
     CDS             complement(4527..5378)
                     /gene="hchA"
                     /locus_tag="RH058_004063"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV3139571.1"
                     /translation="MTVQTSKSPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFHNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGDELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQK
                     MLAAYAG"
     gene            5634..5830
                     /locus_tag="RH058_004064"
                     /pseudo
     CDS             5634..5830
                     /locus_tag="RH058_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5970..7163)
                     /gene="ompC"
                     /locus_tag="RH058_004065"
     CDS             complement(5970..7163)
                     /gene="ompC"
                     /locus_tag="RH058_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3139572.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDFYGKVAGLHYF
                     SDNSGADGDMSYARIGFKGETQIADQFTGYGQWEYNLQANGTEGDGDNSATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFMNGRTNGVATYRNNGFF
                     GQVDGLNFALQYQGNDKSGGPFGQEGSGSGDGRSLSKENGDGFGMSTSYDFDFGLSLG
                     AAYSNSDRSDRQVGVGLNDRNHSNRNAGGETAEAWTVGAKYDANNVYLAAMYAETRNM
                     TPYGGGEFDNGESRSAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGWAHDGN
                     GDPRYTNKDLVKYVEVGATSYFNKNMSTYVDYKINLLDNDDDFYEANGIATDDIVAVG
                     LVYQF"
BASE COUNT         1947 a         1562 c         1706 g         1950 t
ORIGIN      
        1 aacgaaacat aacctgatgc gatctgtgta ggccggatag ggcgctcgcg ccacatccgg
       61 cagggataac gcgtactttg tcggcaatat gaagttatac agataatttg ttgtgaacct
      121 gcttgcgtaa ccggttaaaa agggaacgca acttcttata ccgtaaggct aaaagcagta
      181 cagccagccc agcgtagatg agcggctgcg gtgaaataat cttcacggac cacagataat
      241 gtattggggc cagaatcgcg acaagataga cgaaattatg caactgttgc cagtgcttgc
      301 cgagttttcg ctgcatcgcc tgggttgaag taaacgctaa agcaagcaaa ataacccagc
      361 tgataattcc taacgttaaa taaggccggg taattaactc cttgcctaat aacgccagat
      421 tattcacgcc cagctccagc aatgcgtaac tggttaaatg cagtgtcgcc caggcaaagc
      481 accataagcc taacaggcgg cgagtgcgta tcaataacgg ctgttttgcg tagcgtgcca
      541 gaggtgtgat taacaaggtc gccagcaaaa atttcagtgc agtgcgaccg gtaaaatgct
      601 gaatatcttt caccggatcg gcacccagtc caccgtgatt gatcgcccag accagccaga
      661 gaaacggcaa caatccggca agatgcaggc aaactttcag ccatgtcacc tgttttactg
      721 tcagacgcat tcagaaattc tcccgcaaat ccaggccacg atacagcgat gccacctggt
      781 cggcgtaacc attaaacagt agcgttggct ggcgctgtac atcgaggatg ccgcctgaac
      841 caataaatcg ttcggtagcc tgtgaccagc gtgggtgatc aacatgcgga ttaacgttgg
      901 cgtaaaaacc gtattcgtca ggcgctgcca gattccaggt ggttggcgga cgctcgcggg
      961 tcagcttaat actgactatc gatttaatcc ctttaaagcc atatttccac ggcacaatca
     1021 gtcgtaccgg cgcgccattt tgtggcggta acgccttgcc ataaacaccc acggtcatca
     1081 gtgtgagcgg atgcattgct tcgtcgagac gcaatccttc gacataagga tatttcagcc
     1141 cgccgccgat aaagcggtcc tgctggccag gcatctgttc cggtgcataa attgtttcga
     1201 aagcgacata cttcgcattg ctggtgggtt cggcaagcgc cagcaatttg tgcagcggaa
     1261 aaccaatcca cggcaccacc atcgaccacg cttccacgca gcgcatacga taaatacgct
     1321 cttccagcgg gaagcgacgg gttaaatcat cgtgatcgag ggtcaatggt tttgccactt
     1381 cgccgctgat tttcagtgtc catggatcgg ttttcaggct accagcatta gcggcgggat
     1441 cggctttatc cagcccgaat tcatagaagt tgttatagcc ggaaacttta tctgctggcg
     1501 tcagtggcag gttattttgc caggcggcag gcttgctgaa ctccagcggt tttccggcgg
     1561 gggcgggtgg gcgatcgttc cctttaaacc agctaagcag atcggcatgc gcagcgtgag
     1621 gcaaagaaag tgcagctgcg ctgatgccca gtgcttttaa cacctgccga cgcttcataa
     1681 agaataccga ctcggccgta acatctgatt cttttaaaaa ttgattcttt ttcataagcc
     1741 atcacacttt tttgtcgtta gccagcaaga gccgtttact gttaatctgt cagggagtgt
     1801 cctccctaac agtagcagag atctgctttt acatatttat gcattaactg ccacgatagg
     1861 ttgaataccc atattggcta agtaataaag gcacatgata atgctcgttc actttattaa
     1921 tatgaaattc aacggggatc tcagggaaga aactttcaag attttgtttc ttgaaatagt
     1981 ccccggtttt aaatacgaca cggtaatcgc ccgtagttgc agtttgctcg ggccacagtg
     2041 ccttaattcg tccatcctta tctgttttgg cggtattaag ttgtaaccag ccgttgtccg
     2101 ctttcttttc aagagtgact gtcacgtcgg cagcgggttt tcctgtttgc tggttcaaaa
     2161 tgtgcacgct aagaatgttt tgttgtgcag catagaccaa agaaggtaat gaaaatgcag
     2221 ccgttactac ggagagtact aaataacgct ttaacatgtt tatatccttg tcatgtgaat
     2281 gagttttgag gatagcgaaa aagcgtccag ggtaacctta cagcagcatt acaattttgt
     2341 aatgaaccag gctgtttcta taacatatga tttatggcat attattttca tgaagatttt
     2401 acttattgaa gataatcaaa ggacccagga gtgggtaacg caggggcttt ccgaagcggg
     2461 ttatgtcatt gatgccgttt ctgatggcag agatgggctt tatcttgcgc tgaaggatga
     2521 ttatgcattg atcattctgg atattatgct tccgggtatg gatggctggc agatcttaca
     2581 aacgctaaga acagcaaagc aaacccctgt tatttgcctt actgcaaggg attctgtcga
     2641 tgacagagtc agagggctgg acagtggggc aaatgattat ctggtaaaac ctttttcatt
     2701 ttctgagttg ctggcaaggg ttcgggcaca attaaggcaa catcacgctt tgaattcaac
     2761 attagaaatc agcggcttaa gaatggactc tgttagtcaa agtgtgagca gggacaatat
     2821 cagtattaca ctgacgcgca aggagtttca gttactttgg ctactggcct ccagagctgg
     2881 cgaaattata cccagaacgg ttattgcgag tgaaatttgg ggaatcaact ttgatagtga
     2941 taccaatacg gtggatgtcg ccattcgcag gctccgcgca aaagttgatg atccttttcc
     3001 tgaaaagcta attgccacaa tccgggggat ggggtattca ttcgtagcgg taaaaaaata
     3061 atgaaaagat tatctataac cgtccgttta accttgcttt ttatattgct gctgtctgtt
     3121 gctggcgctg gaattgtctg gactctctat aatggcctcg caagtgagtt gaaatggcgc
     3181 gatgatacaa cactcattaa ccggacagcg cagatcaagc agttgttaat tgatggggta
     3241 aatccagata cgttacctgt atactttaac cggatgatgg atgttagtca ggatatcttg
     3301 atcattcatg gtgatggcat caataaaatt gttaaccgga caaatgtcag tgatgacatg
     3361 ttaaataaca tacctgctag tgagacaatc agcgcagctg gcatttacag aagcattatt
     3421 aatgatactg agattgatgc tttacgaatt aatattgatg aagtttcgcc atcattaacg
     3481 gttactgtgg ctaaattggc ttcagccaga cataacatgc ttgaacagta taaaattaat
     3541 agcattataa tttgcattgt cgccattgta ctttgctcag tattaagtcc gctgttaatc
     3601 agaacgggat tacgagagat caaaaagttg agtggtgtaa cggaagcgct gaattataac
     3661 gatagccgag agcctgttga ggttagcgca ttaccgagag aactaaaacc tcttgggcag
     3721 gcgttgaata aaatgcatca tgctttagtc aaagattttg agcgtctaag tcaatttgct
     3781 gacgatctcg ctcatgaact tagaacgcca attaatgcat tactgggtca gaatcaggtt
     3841 acgctcagtc aaaccagaag tatcgctgaa tatcaaaaaa caattgccgg taacattgaa
     3901 gagctggaaa atatttcgcg gttaacagag aacatactgt ttcttgccag ggcagataaa
     3961 aacaatgttt tggtgaaact ggactcgctt tctctcaata aggaagtcga aaatttgttg
     4021 gactatcttg aatatctttc agacgagaaa gagatttgct ttaaggtcga gtgcaatcag
     4081 caaatctttg cggataaaat tttactgcaa cgaatgttat cgaatcttat tgttaatgcc
     4141 attagatatt caccagaaaa atcgcgtatt catataacca gttttcttga taccaacgga
     4201 tctcttaata ttgatatcgc cagccctgga acgaaaatta atgagcctga aaaactcttc
     4261 cgtagatttt ggcggggaga taattcgcgt cattccgtag gtcagggact aggcctttct
     4321 ttagtcaaag cgattgccga attacatggg ggaagtgcta cgtatcacta tctcaataag
     4381 cataatgtgt tccggattac gttgccgcaa agaaattaac atagggcttc agtacgccat
     4441 aaattaaccg tgaaaaaacg cagccacgtc gtgagtacta acgcggccgc gattgattat
     4501 gcgcttacat tcaaacgtaa cagggattaa cccgcgtaag ctgccaacat tttctgcgcc
     4561 gccagtttac ccaacgcatt cgctgcaaaa ggactgtcgc cggtgagaag tttacggtcc
     4621 ttatgtactc gcccggtgat gtcgtcatta atgatattca tgcccatttt cttcagttcg
     4681 tcgccgaagt accaggtgag atgacccggc atatagccaa tctctggcgt ttgtttgtct
     4741 gcggcgtctg ggaatgcgca aatggaataa ccattcagtg ggttatcgcc gtggcgaagc
     4801 gccagaaaag ccgccgggcc gtggcaaagg gagataacaa aacggtcatt tttgattgcc
     4861 cactgtaaag cggcagccac gtcctggctt tcaggtaaac caataagtgc gccatgacca
     4921 ccaggaacaa agattgctgc atattcgcta tcagcgttga ggctggcgac gacatccgcg
     4981 agtttcttcg gattgtggaa caacgattta tgctgctcaa agaatggcat tactttttca
     5041 tctttgtgcg gcatagccca gtattcaaac ttggtcatca gaccggaaat ggtcgccact
     5101 tcgaattcga aacctgcagc atggagatga tacaacggca gcaacgtttc aatcggatgg
     5161 ttaccggtcg agaacagttt tccgttatcg gtcggcaaat aacgttcgtc cgcggcgatc
     5221 accagaattt tatgtttacc gcgatacggt tttggatagt ccacgccatc cagatcagag
     5281 acaggactgg tatattggct aagcgaatat tctgaaggga agaaggcatt atcttcagca
     5341 atatcgacct gcggactttt acttgtttga acagtcatag tgtattcctt attgttgctt
     5401 agttagggta gtcactatat ttgcgactga gctggtcatt atttttaacc aggctggaaa
     5461 attgagtcaa tatatcgaaa gcggtagtcg gcgggtgtaa gtatgtaatg atgatgcttc
     5521 actggtgttg aatattattc acgccaaatt ttccttattg agcaacgtcg tcgcaagtgg
     5581 cctcaatgat tttagaccat tatcagaggt acggggcggc ggggagagat ttagtgctgt
     5641 ggaatattgt ggtgctgatg aatatttata tcccggattg gtggagaaaa agctttttcc
     5701 atgcactttt tttgcaaata tttctatatg ggaagcgtgt ttgttacgga ttaattcata
     5761 aagaaatgca tttgggtgtt ggcttgaaga gtcgctggtt gatgttattg tgccaggagg
     5821 ggcagggtaa gggggtgtat cgataagctt tggcaattgc gtgtgccgca gatggagatg
     5881 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctagt gttaagtttt
     5941 acagtaacac gccgcggcgt gttggctgat tagaactggt agactaagcc cacagccacg
     6001 atatcatcgg tagcgatgcc gtttgcttcg tagaagtcat cgtcgttgtc cagcaggttg
     6061 attttgtaat caacgtaggt ggacatgttt ttgttgaagt aggaggtcgc accgacttca
     6121 acgtatttaa ccaggtcttt gttggtgtaa cgcggatcgc cattaccgtc atgagcccaa
     6181 ccgcctaaat ccttaccttt agattgcagg taagcgatgg acgggcgcag gccaaagtcg
     6241 aactgatact gagcaacaac ttcaaagttt tgggttttgt tagcgatggc tgatcttgat
     6301 tcaccattat caaactcgcc accaccatat ggggtcatat tgcgagtttc agcgtacata
     6361 gctgctaaat acacattatt agcatcatat tttgcaccaa cagtccatgc ttcagcggtt
     6421 tcaccaccag catttctgtt gctatggtta cgatcattta aaccaactcc aacctgacga
     6481 tctgaacgat cagagtttga atatgcagca cccagactta aaccgaagtc gaagtcgtag
     6541 gatgttgaca taccgaaacc atcaccattt tctttggaaa ggctacggcc atcaccacta
     6601 ccggaacctt cctgcccgaa tggtccacca cttttatcat taccctgata ttgcaaagca
     6661 aagttcaagc catcaacctg accaaagaag ccattgttac ggtaggttgc aacaccgttg
     6721 gtacgaccgt tcatgaagtt gtcggcaccc gcgtaggtat cgccaccaaa ttctggcagc
     6781 atatcggtcc atgcttcgat gtcatatacg acaccgtagt tacgaccata atcgaaagta
     6841 ccattctgac caaaacctaa acctgcgaat gccagacggg tagcagagtt atctccatcg
     6901 ccttctgtgc catttgcttg caggttgtat tcccactgac cgtaaccagt gaactggtca
     6961 gcgatttgtg tttcgccttt gaaaccgata cgggcatatg acatatcgcc atcagcgccc
     7021 gagttgtcag agaagtagtg cagacctgct acttttccgt agaaatccag tttattacca
     7081 tctttattat agacttcagc cgcatttgct gcgccagcaa ctaataacgc cgggaccagc
     7141 attgccagaa cttttctttt catta
//
LOCUS       DAPHDX010000181         7142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPHDX010000181 DAPHDX010000000
VERSION     DAPHDX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.184"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..1450
                     /gene="adeQ"
                     /locus_tag="RH058_004066"
                     /gene_synonym="yicO"
     CDS             116..1450
                     /gene="adeQ"
                     /locus_tag="RH058_004066"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV3139573.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1503..1955
                     /locus_tag="RH058_004067"
     CDS             1503..1955
                     /locus_tag="RH058_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV3139574.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2166..3356
                     /gene="nepI"
                     /locus_tag="RH058_004068"
     CDS             2166..3356
                     /gene="nepI"
                     /locus_tag="RH058_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV3139575.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3397..3690)
                     /locus_tag="RH058_004069"
     CDS             complement(3397..3690)
                     /locus_tag="RH058_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139576.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3912..4730
                     /gene="nlpA"
                     /locus_tag="RH058_004070"
     CDS             3912..4730
                     /gene="nlpA"
                     /locus_tag="RH058_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV3139577.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSGSDTKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKSVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4734..5657)
                     /locus_tag="RH058_004071"
     CDS             complement(4734..5657)
                     /locus_tag="RH058_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3139578.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5768..6952)
                     /locus_tag="RH058_004072"
     CDS             complement(5768..6952)
                     /locus_tag="RH058_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV3139579.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFCLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1797 a         1598 c         1801 g         1946 t
ORIGIN      
        1 gattattaaa gaatatccat taatgtgcaa ttgaaatgtg ataattatca ctgaaccgtt
       61 gttgtcattt gttttacaaa agcaagggat taaatctcct ccaatggaca aaaaaatgaa
      121 taatgacaat accgattatg tgagtaatga atcagggacg ctttcgcgat tatttaaact
      181 acctcagcat gggaccaccg tccgcacaga attgattgcg gggatgacca cttttttaac
      241 catggtgtac atcgtttttg tgaacccgca aatcctcggc gcggcacaaa tggacccgaa
      301 agtggtgttt gttaccacct gtttgattgc cggtatcggc agtattgcga tggggatatt
      361 tgctaactta cccgtggcgc tggctccggc aatggggctg aacgccttct ttgccttcgt
      421 ggtcgtgggg gcgatgggca tctcctggca gaccgggatg ggcgcaatat tctggggcgc
      481 agttggacta tttttgctca cgctgtttcg tatccggtac tggatgatct ccaacattcc
      541 cttaagttta cgtattggta tcaccagcgg aattggatta tttatcgcct taatgggatt
      601 aaaaaatact ggcgttattg tcgccaataa agacacgctg gtgatgattg gcgatttaag
      661 ttctcacggc gtgttgttag gtattttagg gttttttatt ataaccgtgt tgtcatcacg
      721 tcattttcat gccgcggtgc tggtttctat tgtggtgacg tcttgctgtg gattattttt
      781 cggtgatgtt cattttagcg gcgtctattc cattccgcct gatattagcg gcgtcattgg
      841 tgaagtagat ttgagcggcg cgttaacact tgaactcgcc ggtatcattt tctcctttat
      901 gctgatcaac ctatttgatt catcaggaac attaattggt gtaactgata aagcgggctt
      961 aatagatggt aacggtaaat tccccaatat gaataaggcg ctgtatgttg atagcgtcag
     1021 ttcggtggcg ggtgcgttta tcggcacctc gtctgttact gcctatattg aaagtacttc
     1081 tggtgtggca gtcggtggcc gcacggggct gactgcggtt gtggttggcg ttatgttcct
     1141 gttggttatg ttcttctcac cgctggtggc gatggttcct ccttacgcaa ccgccggagc
     1201 gttaatcttt gttggcgtgc tgatgacttc gagcctggcg cgcgttaact gggatgattt
     1261 taccgaatcg gtgcctgcgt ttattaccac ggtgatgatg ccctttactt tctcgatcac
     1321 cgaagggatt gcactcggct ttatgtcgta ctgcatcatg aaagtgtgca ccgggcgctg
     1381 gcgcgatctg aacctgtgtg tggtggtggt cgcagctctg tttgcactga agattattct
     1441 ggtggattag taactggcga gggtttccag atatcatgag ttctgattac gcaggagaac
     1501 tcatgatctg gataatgctc gccacgctgg cggtagtgtt tgtggttggt tttcgggtgc
     1561 tgacatccgg ggccagaaaa gcgattcgcc gtctcagcga tcggctgaat atcgatgtcg
     1621 tacccgtgga gtcgatggtc gatcaaatgg gaaagtcagc cggtgacgaa tttttacgtt
     1681 atttgcatcg cccggatgag tcgcacctgc aaaacgccgc gcaggtgttg ctcatctggc
     1741 aaattgtcat tgtcgatggt agcgaacaga acctgctgca atggcatcgg attttacaaa
     1801 aagcccgtct tgccgcgccg attaccgatg ctcaggtcag gctggcgctg ggttttctgc
     1861 gcgaaaccga acctgaaatg caggatatta atgcttttca gatgcgctat aacgcgttct
     1921 ttcagcctgc cgagggcgtt cactggctgc attaaaaccg cttcgtctta tcagcctaca
     1981 gaccgcgcct gacctgtcgg gtggataaga cgttcacgcc gcatccggta atctttgccc
     2041 tgttccgttt cgatcttaac tccagctcaa gcaacaccgt aaaagttgca aaaacgttaa
     2101 atgcgtcaca catttcaatg ttaaagtggt cggcttttcc cctgaaacat gccacgggta
     2161 acaccatgag tgaatttatt gccgaaaacc gcggcgcgga tgccatcacc cgaccgaact
     2221 ggtcagccgt tttctcggtg gcgttttgcg tcgcctgtct gattatcgtt gagtttttgc
     2281 ccgtcagttt gttgacgcct atggcccagg atttaggcat ttcggaaggg gttgccgggc
     2341 aatcggtgac cgtgaccgcc tttgtggcaa tgtttgccag tttgtttatt acccagacaa
     2401 ttcaggccac tgaccgccgc tacgttgtta ttttgtttgc cgttttgctg acgctctcct
     2461 gtttgctggt ttcctttgcc aactcattca gtttgctttt aatcggtcgt gcctgtctgg
     2521 ggctggcgct gggcgggttc tgggcgatgt cggcgtcgct gaccatgcgt ctggtaccgc
     2581 cgcgtacggt gccgaaggcg ctgtcggtga tcttcggcgc ggtttctatt gcgctggtga
     2641 ttgccgcgcc gttgggcagt tttttaggcg agcttatcgg ttggcgcaat gtctttaatg
     2701 cggcggcggt gatgggcgtg ttgtgtattt tctggattat caaatcattg ccttcactgc
     2761 caggcgaacc ctcgcatcag aaacaaaata ctttccgctt attacaacgt ccgggtgtga
     2821 tggcagggat gatcgccatc ttcatgtctt tcgccgggca gtttgctttc ttcacgtata
     2881 ttcgcccggt gtatatgaac ctggcgggat tcggcgtgga tggcttaacg ctggtgctgt
     2941 tgagttttgg tatcgccagc tttattggta cgtcgctttc gtcgttcatt cttaaacgtt
     3001 cggtaaaact ggccttagca ggcgcgccgt taatactggc tgtgagtgcg ttggtactga
     3061 cgttgtgggg aagcgataaa atcgttgcta ccggcgtggc gattatctgg gggctaactt
     3121 ttgcattggt tcccgtcggc tggtcaacgt ggatcacccg ctcgctggcc gatcaggcag
     3181 aaaaagccgg gtctattcag gtggcggtta ttcagcttgc taatacctgt ggcgcggcaa
     3241 tcggcggtta tgcgctggat aatattggtc tgacttcgcc gttgatgttg tccggcacat
     3301 tgatgttgct gactgcattg ttggttactg caaaggtgaa aatgaagaaa tcctgatatc
     3361 aggtaaagaa gcgttggtca gtagttgttg ctgttcttac atatccgggc attctacctt
     3421 acccaacgct tcccaataat tgctctgatt attacgttcc atcgccacga tggcttcgcg
     3481 tatatgttgt ttattgtttt catctgacaa cattaacttt tcccacgctt catctgataa
     3541 tgacgcctgt ggcgtgcaaa ttttacgtaa atcttgtaat accgtggttt tctcttttgc
     3601 ggactgcagt gagctaaaag gcgactcggc gtatacgatg cctggcatag cgaaaaaagt
     3661 gaaaataagc agtagtgtcg ttggtttcat ggattcaacc ttttacaaat gtgtatcgaa
     3721 tgaagcataa gtgtagtttg ctttcatcgc tctcatcatg ctgtacgaaa cgcgcttgcg
     3781 tggtcagtaa gaagtgccag actttatatt ccacttttat tcctttttat tcttatcgat
     3841 agcgtttcgt tttttaaacc gcagcgacct taccgctata gtcaggtaat cattaataaa
     3901 aggataaaaa agtgaaactg acaacacatc atctacgggc gggggccgca ttattgctgg
     3961 caggaattct gctggcaggt tgcgaccaga gtggcagcga tacaaaacac attaaagttg
     4021 gcgttataaa tggcgcagaa caagatgtcg cggaagtcgc taaaaaggta gcaaaagaga
     4081 aatatggtct cgatgttgaa ctggtaggat ttagcggttc gttgctaccg aacgatgcga
     4141 caaatcatgg cgaacttgat gcgaatgtct ttcagcatcg accctttctc gaacaagata
     4201 atcaggcgca tggttataaa ctggtagcgg tgggaaatac gtttgtcttc cccatggcgg
     4261 gttattccaa aaaaatcaaa agtgtggcgc aaataaaaga aggcgcgaca gtggcgatcc
     4321 ccaacgaccc gaccaacctt ggtcgtgcgc ttttactgtt gcaaaaagag aagctaatta
     4381 ctctgaaaga gggaaaaggg ttattaccta ccgcgctgga tataaccgat aatcctcgtc
     4441 atttgcagat tatggaactc gagggggcgc agctcccgcg cgtactggat gatcccaaag
     4501 ttgatgtagc gattatcagc accacttaca ttcagcagac agggctttct ccggtgcacg
     4561 acagcgtatt tattgaagat aagaattcgc cgtatgtgaa tattttggtg gcacgggaag
     4621 ataataagaa tgcagaaaac gtgaaggaat ttctgcaatc ttatcaatca cccgaagtcg
     4681 ctaaagcggc agaaaccatt tttaacggtg gcgcggtgcc tggctggtaa ttctcactca
     4741 tgccgcgccg gacgatttat ttttctggcg cggcgacggg aatccattga aatcaaaatc
     4801 accgatgaca gaatcagcag cgttcccagc cagtccggta atgtgaacgt gatccccagc
     4861 aacagcaaag agagtagcgc gctacttaat ggttctgcac agctcaaaat gctggctttt
     4921 ggaccgccaa ttaattgtgc tcctttcagg tacagactaa atgtcaggga cgtaccaatg
     4981 accaccaaat aaaaaaacgc cagaatcaaa ctgccgttaa cgacaaagtt tgttccttgt
     5041 ctggcataaa aaggcaacag aatcagaccg ccaatcagca tactccagcc gacgactggt
     5101 aacgtgccat agcgggcaat tagcgttgag ggataggtgg tatagaatgc agcagcaaat
     5161 gccgaggcaa tgccccagaa caacgcggca ggagagatcg ataatgacgt cggattaccg
     5221 tgtgtcacca ataaaaaagt cccgaccagc gatgtcaaaa tagcgcagaa aaccagaatg
     5281 cccgggcgcg atttacgcac cagtgagaac caggcgacga taatcgtcgg tgagaggaat
     5341 tgcagcaccg ttgccgtggc tgcgttcgat ttttcgatgg ttagcaaaaa agtgagctgt
     5401 acagttagcg cgccaaccac ggaaaaaatc agcaggctaa tggcatcttt atgattgtta
     5461 ataatagaaa agattttatc gccatgaaca aatgacagcg tcagtagaat caaaccggcg
     5521 aatatcaaac gcgtcatagt caaaaactgc gacgacatct ggctttgctc catgatgtat
     5581 tgcgcgcaga cccctgaact tccccacaac acggcggcaa tcagaacgtt cagcatcccc
     5641 tttctggtgg aacccatttt tccctctgca tactgttttg tcgtttatgt atgcaggcat
     5701 gatagcaaaa atggcgagga tggctctgtt tgcgccacgc cgatttttct tagcccagac
     5761 tggtgagcta aatatcttta ataaacagca ggcaaatcat cgcaataccc aacatcccta
     5821 tcgccagaca gaacaacgca tggtagctcc agatttcaac cataattccg tcaacagagc
     5881 cggcgataat ccagccgacg cgtgaagtat ttgcatataa cgttgtcgca gagcctattt
     5941 tttcaggcat caagtcctga aaataaagca tgccgatacc acagagaata ccaaggaaga
     6001 tggcattaag aatttgcaat tccagctcaa ccgccggagt cgtcgccatg agtacgctgg
     6061 cgtaaaaaca cattccactc acgatagcaa tgagcattaa tagtcgcttg ccaatacgtt
     6121 tcatgtaata gcctgcgatt aacatcatcg gaatttccag accggcagcg ataccaatca
     6181 tttctccagc cagtttatcg gttagatgca gttcatcaat aataaatagc ggcatattta
     6241 tcatgtagag attattcgcc gcccacatca ttgaacagac cacaaaaagt agccgcgtat
     6301 cccgcttcct gtgggtggag ggtaaaattt ctacgggttg ggtaacgaca ggaatatttc
     6361 tttgtattga tggcaaaaac aaccagacta tcagtccgca aacaacaaat gcgatggctg
     6421 cggtgagata catcacttta aaactaaatc ccattgccag ttcataagcg agcggtggcc
     6481 cgataaccca ggcaagcgag atctgagcac gtaaaaatgt actgaacatg accgtctcac
     6541 ggcctgttct gtcggcgtgt tcacgggcga gggcgaacat ttgcgggttt gcggtggaag
     6601 caaaactact cagaagtacg cccgttgaga ggagaatgaa gtagttgcga ttccacgcaa
     6661 aaagcgtgca ggccagcact ccaaataagc aacatagcag aatcagtaat ttacggtcgc
     6721 cttgtttatc ggagtgcctt gccagaaatt gactgaccag aattcccata atagcgctac
     6781 cggtgaagaa aaaacctacc attataggac gggctttcag ttcatctgcg aggaatatac
     6841 ttagggtagg agtctgaaga gcgcccgcaa tacccgtcag aaaggcgaca agtaaaaatg
     6901 ccgcggcagt gagatcaagt atttttgatg gagtggtagc cgttttttgc attcttctga
     6961 atcaccgggc gaaaaaatat aactgagtta caaataagta ccagtcgaat gagtattgta
     7021 ttaagaattt ttgagtatcg ctgatacaac cagaaagcaa tgctagcgca atattatagg
     7081 gccagcaatt gagattatta gtaaaagcat gttaagaatt aattaccgaa tttatatatt
     7141 aa
//
LOCUS       DAPHDX010000182         7117 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPHDX010000182 DAPHDX010000000
VERSION     DAPHDX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.185"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(305..1441)
                     /locus_tag="RH058_004073"
     CDS             complement(305..1441)
                     /locus_tag="RH058_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3139580.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLKMTSWMHYLYLDH"
     gene            complement(1444..1806)
                     /locus_tag="RH058_004074"
     CDS             complement(1444..1806)
                     /locus_tag="RH058_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV3139581.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2343..4256
                     /gene="mtlA"
                     /locus_tag="RH058_004075"
     CDS             2343..4256
                     /gene="mtlA"
                     /locus_tag="RH058_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV3139582.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4351..5499
                     /gene="mtlD"
                     /locus_tag="RH058_004076"
     CDS             4351..5499
                     /gene="mtlD"
                     /locus_tag="RH058_004076"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV3139583.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5499..6086
                     /gene="mtlR"
                     /locus_tag="RH058_004077"
     CDS             5499..6086
                     /gene="mtlR"
                     /locus_tag="RH058_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV3139584.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6098..6307)
                     /locus_tag="RH058_004078"
     CDS             complement(6098..6307)
                     /locus_tag="RH058_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139585.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6592..6954
                     /locus_tag="RH058_004079"
     CDS             6592..6954
                     /locus_tag="RH058_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV3139586.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1811 a         1792 c         1801 g         1713 t
ORIGIN      
        1 tttaaatgtg actgtcatcc ttcagataat aaagccatca tcgcaataga tggaaaagca
       61 ctcctgctct tttttgatta gagccgtcgc aggaggaaca actcatatca tcattgcgat
      121 ctcgacgatg tatagtctca tcctgcggca gaacaagacg gataaaaaat ccaatgagag
      181 cacactcatc cagaacttat taacataatg aatattaaag aaaaatcatc gcagctaatg
      241 aagtaatatg caaaaataat tagtccttac aaggagcaaa gttccataat ctcccccctc
      301 cccctcaatg atccaaataa agataatgca tccagctggt catttttagc agcactttcc
      361 gcatcaccga aacatggctg aaatggtcgg agtaaaccgc cacctgggcg tagatgccgt
      421 cgggtaaggc atcgatatca tcgttagggt ccagttcaat ggttcccagc acaccgtccg
      481 tgccgggcac gaccgttaat gattgcaata ccccctgcgc ctgataagaa ccgcctggca
      541 cgacaggtaa aatactagtc agtttgccgt gaaacacctg cccaggtagc gcgttaaaca
      601 ccacttccgc atcatcgccg ggtttcagac gtaacagcga gttttgccga aattgggcga
      661 caatttgccg tttttgctcg gggatgaaga ccatcaccgg acgcagcggc aaggcagctg
      721 cgtatgtacc tgggcggatc agtacctgag taacgtagcc attgctcggc gcgcgaatga
      781 cagtctgctc aaggttatat tttgcttcag taagttgcgc tcttaagctc acaatctgcg
      841 attgctcgcc gttaaccata ctgtcgagct ggctctggat ctgcgcctgc tccgccaccg
      901 agcctttcac cagcgcatcc tgcgcgagga aattttgccg cgcatcgtcg atgtcacgtt
      961 ccgagaacgg attcaccgcc gcctggctgc ctttcaagta acgttgataa tttttaaaca
     1021 gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg cgcttcagtg agctgcgcac
     1081 gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc ctgaagtctg tcaactcgcg
     1141 cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc cttttgaata agctgattat
     1201 tcttgtcagt gacttcagta acaattcccg tcacctgtgg cgtgataggg atcgctatca
     1261 ctgccttttg cgcggtaaaa gtgtaagggt ggttgtagtt catcaacaaa atcaaaccac
     1321 tcaccagaaa cacgcctccc agcgccgccg tcgccagcgt ccactgattt accggaatgc
     1381 ggaagatttt aaagaccgcc cacgccagcg ccacgtaagt taaaacaatc aatagatcca
     1441 taattagatc tccgggaacg tggaatagtc agtctttttc tcggcggcga gttgatgctc
     1501 cagccgggaa attcggtcag aaagtgcggc gatttccggg tcagtcgctt tctcctgcga
     1561 cgcaacatgt gactgcatac cccagccacg ctccggttga tagagcgtcg cccagatcca
     1621 cagaaacggc cagataacat gcagagtaaa caggctcacc cagcccgccg tatgaatagc
     1681 gtcggcatgg ggatggttgc gctttttggc aatcagataa gggatgtcat gtatcgcgat
     1741 gattccataa aaaatcacca gaaagacgaa gatcagcact cccaacgcga aatagtttag
     1801 aaacatatct gcctcggatt cacgtttatc agtgttgttt ttgggctggc agccagaagg
     1861 gagtcaggct gatattttga caataatccg ggttcgcgat tctcgccata acaccaaaga
     1921 ataattttta gaggtgatga gttgcttagt tacataacga ttgtatgacg aaggcataac
     1981 atgctgtaga tcacatcagg tgaacgccgt aagaaaatat cttgtgattc agatcacaaa
     2041 gattcaacaa accatcaaaa caaaaatgtg acactactca catttaaatg ccatttttag
     2101 cgaaaatcgc cgccttgttg cttttttaca caagcgtttt gtgatgaacg tcacgtcaat
     2161 tacctctcta ccccctatat ttatgtgatt gatatcacac aaaaggccgt cgactggaca
     2221 gttaaccgat tcagtgccag atttcgcagt atctacaagg tccggctacc tctgccgcca
     2281 cattaacaaa aaacctcggg cttccagcct gcgcgacagc aaacataaga aggggtgttt
     2341 ttatgtcatc cgatattaag atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg
     2401 tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta tttattccaa
     2461 cagggtggtt accgaacgag acgctggcga agctggtcgg gccgatgatc acttatctcc
     2521 tgccgctgct gatcggttat accggtggta agctggtagg cggcgaacgt ggcggcgtag
     2581 tcggtgccat caccaccatg ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt
     2641 ctatgattgc aggtccgctg ggcggctggt gcattaagca cttcgaccgc tgggtagacg
     2701 gtaagatcaa atccggtttt gagatgctgg tgaataactt ctccgcaggc atcatcggga
     2761 tgatcctcgc tattctggca ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa
     2821 tgctggctgc gggcgttaac ttcatggttg tccatgacat gctgccgctg gcgtctatct
     2881 ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat taaccacggt atcttctcgc
     2941 cgctgggtat tcagcagtcc catgaactgg gtaaatcaat cttcttcctg attgaagcta
     3001 acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta
     3061 aacagtctgc gggcggtgcg gcaatcatcc acttcctggg tggtatccac gaaatctact
     3121 tcccgtatgt gctgatgaat ccgcgtctga tcctcgcagt catcctcggc ggtatgactg
     3181 gcgtgttcac gctgactatc ctgggcggtg gtctagtttc tccggcatct ccgggttcta
     3241 tccttgctgt actggcgatg acaccaaaag gtgcttactt cgctaacatc gcgggtgtgt
     3301 gtgcggcgat ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga
     3361 aagaagaaga tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta
     3421 aaggcgcatc tccgctgtct gctggcgatg tgactaacga cctgagccac gtacgtaaaa
     3481 tcatcgttgc ctgtgacgcc ggtatgggtt ccagtgcgat gggcgcaggc gttctgcgta
     3541 agaaaattca ggatgcaggt ctgtcgcaga tttctgttac taacagcgcg atcaacaacc
     3601 tgccgccaga tgtggacctc gtcatcactc accgtgacct gaccgaacgc gctatgcgcc
     3661 aggttccgca ggcacagcat atttcgctga ccaacttcct cgacagcggc ctgtacacca
     3721 gcctgaccga acgtctggtt gctgcccaac gccacacggc aaacgaagag aaagtaaaag
     3781 acagcctgaa agacagcttt gacgattcca gtgctaacct gttcaagcta ggcgcggaga
     3841 acatcttcct cggtcgcaaa gcggcaacca aagaagaagc gattcgtttt gctggcgagc
     3901 agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa
     3961 aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga
     4021 aagatcgcgt actgaaaacg ggcgtcgtgt tctgccagta cccggaaggc gtgcgcttcg
     4081 gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc
     4141 acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc
     4201 tggcacacac caccagcgtg gatgaagtgc tggaactgtt ggcaggtcgt aagtaatcca
     4261 atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct
     4321 cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat
     4381 atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc
     4441 gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg
     4501 gttggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt
     4561 gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt
     4621 gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt
     4681 aatgaatccc cgttgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg
     4741 aaaggccatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt
     4801 ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat
     4861 ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa
     4921 ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa
     4981 cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc
     5041 ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt
     5101 gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat
     5161 gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta
     5221 gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca
     5281 ttgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt
     5341 gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct
     5401 gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt
     5461 gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca
     5521 ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct
     5581 ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca
     5641 ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag
     5701 acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg
     5761 atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg
     5821 aagatgcgga gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg
     5881 cctttaccga cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac
     5941 caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct
     6001 atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca
     6061 gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg
     6121 gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg
     6181 gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct
     6241 ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt
     6301 tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt
     6361 ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc
     6421 tgctgtttat tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt
     6481 aagcatgtta atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac
     6541 ctggtcgcgc atactagggg ctataaattt atctttatca gaagccatca catgaaagaa
     6601 gtcgaaaaaa acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag
     6661 gccgatctgt cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc
     6721 acgctggaag cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa
     6781 gcacagaagc tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat
     6841 atgggcaagc tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg
     6901 ggccgtgaaa tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct
     6961 cagacgactg gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg
     7021 gactctcctg tctactcgcc taattaccaa acaattcact ccctttactt cgatttaccg
     7081 ctggttgata ttctgaaaat gcatttttac cgaaata
//
LOCUS       DAPHDX010000183         7042 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPHDX010000183 DAPHDX010000000
VERSION     DAPHDX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.186"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            559..1548
                     /gene="araF"
                     /locus_tag="RH058_004080"
     CDS             559..1548
                     /gene="araF"
                     /locus_tag="RH058_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV3139587.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1618..3132
                     /gene="araG"
                     /locus_tag="RH058_004081"
     CDS             1618..3132
                     /gene="araG"
                     /locus_tag="RH058_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV3139588.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3147..4133
                     /gene="araH"
                     /locus_tag="RH058_004082"
     CDS             3147..4133
                     /gene="araH"
                     /locus_tag="RH058_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV3139589.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4300..5100
                     /gene="otsB"
                     /locus_tag="RH058_004083"
     CDS             4300..5100
                     /gene="otsB"
                     /locus_tag="RH058_004083"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV3139590.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5075..6499
                     /gene="otsA"
                     /locus_tag="RH058_004084"
     CDS             5075..6499
                     /gene="otsA"
                     /locus_tag="RH058_004084"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV3139591.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6506..6934)
                     /gene="uspC"
                     /locus_tag="RH058_004085"
     CDS             complement(6506..6934)
                     /gene="uspC"
                     /locus_tag="RH058_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV3139592.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1780 a         1635 c         1825 g         1802 t
ORIGIN      
        1 agtgcttttt gtatcttatt gatattaatc aatgaatttg aattcatgat gaagtgatga
       61 ttttataaaa cgttttttca tttttgcgag atggctctca ttatacgtgt tctgttaatt
      121 aatgagcaca gtgataatta atggcgaaat gaatgacgtg cattttccac atctttgagt
      181 tgcggttatt acaccatttc aaaaactcaa cgccaggtaa tgcggcctat tgactggtta
      241 aaaagaagac atcccgcatg ggtaccaaag acaacaagga tttccaggct aatcttatgg
      301 attaatctgc tgtgcattcg acaatttgtc tgacaaattg gctttccctt atgtcttttc
      361 ccgctaaatt tatgcacgtt ctcactgtaa ttctgcgatg tgatattgct ctcctatgga
      421 gaattaattt ctcgctaaaa ctatgtcaac acggtcactt atcttttaga taaaaggtaa
      481 tgctttgttt tccgattaat ttaacgaaag tcattcgttt ttgccctaca caaaacgaca
      541 ctaaagctgg agagaaccat gcacaaattt actaaagccc tggcagccat tggtctggca
      601 gccgttatgt cacaatccgc tatggcggag aacctgaagc tcggttttct ggtgaagcaa
      661 ccggaagagc cttggttcca gaccgaatgg aagtttgccg ataaagccgg gaaggattta
      721 gggtttgagg ttattaagat tgccgtgccg gatggcgaaa aaacattgaa cgcgatcgac
      781 agcctggctg ccagtggcgc aaaaggtttc gttatttgta ctccggaccc caaactcggc
      841 tctgccatcg tcgcgaaagc gcgtggctac gatatgaaag tcattgccgt ggatgaccag
      901 tttgttaacg ccaaaggtaa gccaatggat accgttccgc tggtgatgat ggcggcgact
      961 aaaattggcg aacgtcaggg ccaggaactg tataaagaga tgcagaaacg tggctgggat
     1021 gtcaaagaaa gcgcggtgat ggcgattacc gccaacgaac tggataccgc ccgccgccgt
     1081 actacgggat ctatggatgc gctgaaagcg gccggattcc cggaaaaaca aatttatcag
     1141 gtacctacca aatctaacga catcccgggg gcatttgacg ctgccaactc aatgctggtt
     1201 caacatccgg aagttaaaca ttggctgatc gtcggtatga acgacagcac cgtgctgggc
     1261 ggcgtacgcg cgacggaagg tcagggcttt aaagcggccg atatcatcgg cattggcatt
     1321 aacggtgtgg atgcggtgag cgaactgtct aaagcacagg caaccggctt ctacggttcc
     1381 ctgctgccaa gcccggacgt acatggctat aaatccagcg aaatgcttta caactgggta
     1441 gcaaaagacg ttgaaccgcc aaaatttacc gaagttaccg acgtggtact gatcacgcgt
     1501 gacaacttta aagaagaact ggagaaaaaa ggtttaggcg gtaagtaatt tgccggaaaa
     1561 attcccctct gcatgatgca gagggggtgt gaacgaccag tgattcacgg agacgttatg
     1621 caacagtcta ccccgtatct ctcatttcgc ggcatcggta aaacgtttcc cggcgttaag
     1681 gcgctgacgg atattagttt tgactgctat gccggtcagg ttcatgcgtt gatgggtgaa
     1741 aatggcgcag gaaaatcaac tctcttaaaa atcctcagcg gcaactatgc gccaaccacg
     1801 ggttctgtag tgattaatgg gcaggaaatg tccttttccg acacgaccgc agcacttaat
     1861 gcgggtgtgg cgattattta ccaggaactg catctcgtgc cggaaatgac cgtcgcggaa
     1921 aacatctatc tcggccagct gccgcataaa ggcggcattg tgaatcgctc attgctgaat
     1981 tatgaggcgg gtttacaact taaacatctt ggtatggata ttgacccgga cacgccgctg
     2041 aaatatctct ccattggtca gtggcagatg gttgaaatcg ccaaggcgct ggcgcgtaac
     2101 gctaaaatta tcgcctttga tgagccaacc agctcccttt ctgcccgcga aatcgacaat
     2161 cttttccgcg ttattcgtga actgcgaaaa gaggggcggg tgatcttata cgtttctcac
     2221 cgtatggaag aaatatttgc cctcagcgat gccatcactg tctttaaaga tggacgttat
     2281 gtcaaaacct ttaccgatat gcagcaggtt gaccacgacg cgctggtgca ggcgatggtc
     2341 gggcgcgaca ttggcgatat ctacggctgg caacctcgta gttatggcga ggagcgcctg
     2401 cgtcttgatg ctgtgaaagc accaggcgtg cgtacgccaa taagtctggc ggttcgcagt
     2461 ggtgaaattg ttgggctgtt tggtctggta ggagcggggc gtagcgaatt aatgaaaggc
     2521 ttgtttggcg ggacgcaaat caccgccggt caggtttata tcgaccaaca gccgatcgat
     2581 attcgtaaac cgagccacgc cattgccgca ggcatgatgc tctgcccgga agatcgcaaa
     2641 gcggaaggca ttattcccgt gcactccgtt cgcgacaata tcaacatcag tgccagacgt
     2701 aaacatgtgc tcggcggttg tgtaatcaac aacggttggg aagaaaacaa tgccgatcac
     2761 cacattcgtt cgctcaacat caaaacgccg ggcgctgagc aactgatcat gaatctctca
     2821 ggcggaaatc agcaaaaagc cattctgggc cgctggttat cggaagagat gaaggtcatt
     2881 ttgctggatg aacctacgcg cggcattgat gttggtgcta agcatgaaat ttacaacgtg
     2941 atttatgcgc tggcggcgca gggtgtggcg gtgctgtttg cctccagcga cttacctgaa
     3001 gtcctcggcg ttgccgaccg gattgtggtg atgcgggaag gtgaaatcgc cggtgaattg
     3061 ttacacgagc aggcagatga gcgtcaggca ctgagccttg cgatgcctaa agtcagccag
     3121 gcagttgcct gagtaaggag agtatgatgt cttctgtttc tacatcgggg tctggcgcac
     3181 ctaagtcgtc attcagcttc gggcgtatct gggatcagta cggcatgctg gtggtgtttg
     3241 cggtgctctt tatcgcctgt gccatttttg tcccaaattt tgccaccttc attaatatga
     3301 aagggttggg cctggcaatt tccatgtcgg ggatggtggc gtgtggcatg ttgttctgcc
     3361 ttgcttccgg tgactttgac ctttctgtcg cctccgtaat tgcctgtgcg ggtgtcacca
     3421 cggcggtggt tatcaacctg actgaaagcc tgtggattgg cgtggcagcg gggttgctgc
     3481 ttggcattct ctgtggcctg gtcaatggct ttgttatcgc caaactgaaa ataaatgctc
     3541 tgatcacaac actggcaacg atgcagattg ttcgaggtct ggcgtacatc atttcagacg
     3601 gtaaagcggt cggtatcgaa gatgaaagct tctttgccct tggttacgct aactggttcg
     3661 gtctgcctgc gccaatctgg ctcaccgtcg cgtgtctgat tatctttggt ttgttgctga
     3721 ataaaaccac ctttggtcgt aacaccctgg cgattggcgg gaacgaagag gctgcgcgtc
     3781 tggcgggtgt accggttgtt cgcaccaaaa ttattatctt tgttctctct ggcctggtat
     3841 ctgcgatagc cggaattatt ctggcttcac gtatgactag tgggcagcca atgacgtcga
     3901 ttggttatga gcttattgtt atctccgcct gcgttttagg tggcgtttct ctgaaaggtg
     3961 gcatcggaaa aatctcatat gtggtggcgg gtatcttaat tttaggcacc gtggaaaacg
     4021 ccatgaacct gcttaatatt tctcctttcg cgcagtacgt ggttcgcggc ttaatcctgc
     4081 tggcagcggt gatcttcgac cgttacaagc aaaaagcgaa acgcactgtc tgatgctttt
     4141 ttccacaaca atttaacgtt ttttcccacc acagccagcc gccacaacgg ttggctgttc
     4201 ttcattgcaa atggcgaccc ccgtcacact gtctatactt acatgtctgt aaagcgcgtt
     4261 ctgcgcaaaa caataagaaa agagaaggag gagagacggg tgacagaacc gttaaccgaa
     4321 acccctgaac tatccgcgaa atatgcctgg ttttttgatc ttgatggaac gctggcggaa
     4381 atcaaaccgc atcccgatca ggtcgtcgtg cctgacaata ttctgcaagg actacagcta
     4441 ctggcaaccg caagtgatgg tgcattggca ttgatatcag ggcgctcaat ggtggagctt
     4501 gacgcactgg caaaacctta tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt
     4561 gacatcaatg gtaaaacaca tatcgttcat ctgccggatg cgattgcgcg tgatattagc
     4621 gtgcaactgc atacagtcat cgctcagtat cccggcgcgg agctggaggc gaaagggatg
     4681 gcttttgcgc tgcattatcg tcaggctccg cagcatgaag acgcattaat gacattagcg
     4741 caacgtatta ctcagatctg gccacaaatg gcgttacagc agggaaagtg tgttgtcgag
     4801 atcaaaccga gaggtaccag taaaggtgag gcaattgcag cttttatgca ggaagctccc
     4861 tttatcgggc gaacgcccgt atttctgggc gatgatttaa ccgatgaatc tggcttcgca
     4921 gtcgttaacc gactgggcgg aatgtcagta aaaattggca caggtgcaac tcaggcatca
     4981 tggcgactgg cgggtgtgcc ggatgtctgg agctggcttg aaatgataac caccgcatta
     5041 caacaaaaaa gagaaaataa caggagtgat gactatgagt cgtttagtcg tagtatctaa
     5101 ccggattgca ccaccagacg agcacgccgc cagtgccggt ggccttgccg ttggcatact
     5161 gggggcactg aaagccgcag gcggactgtg gtttggctgg agtggtgaaa cagggaatga
     5221 ggatcagccg ctaaaaaagg tgaaaaaagg taacattacg tgggcctctt ttaacctcag
     5281 cgaacaggac cttgacgaat actacaacca attctccaat gccgttctct ggcccgcttt
     5341 tcattatcgg ctcgatctgg tgcaatttca gcgtcctgcc tgggacggct atctacgcgt
     5401 aaatgcgttg ctggcagata aattactgcc gctgttgcaa gacgatgaca ttatctggat
     5461 ccacgattat cacctgttgc catttgcgca tgaattacgc aaacggggag tgaataatcg
     5521 cattggtttc tttctgcata ttcctttccc gacaccggaa atcttcaacg cgctgccgac
     5581 atatgacacc ttgcttgaac agctttgtga ttatgatttg ctgggtttcc agacagaaaa
     5641 cgatcgtctg gcgttcctgg attgtctttc taacctgacc cgcgtcacga cacgtagcgc
     5701 aaaaagccat acagcctggg gcaaagcatt tcgaacagaa gtctacccga tcggcattga
     5761 accgaaagaa atagccaaac aggctgccgg gccactgccg ccaaaactgg cgcaacttaa
     5821 agcggaactg aaaaacgtac aaaatatctt ttctgtcgaa cggctggatt attccaaagg
     5881 tttgccagag cgttttctcg cctatgaagc gttgctggaa aaatatccgc agcatcatgg
     5941 taaaattcgt tatacccaga ttgcaccaac gtcgcgtggt gatgtgcaag cctatcagga
     6001 tattcgtcat cagctcgaaa atgaagctgg acgaattaat ggtaaatacg ggcaattagg
     6061 ctggacgccg ctttattatt tgaatcagca ttttgaccgt aaattactga tgaaaatatt
     6121 ccgctactct gacgtgggct tagtgacgcc actgcgtgac gggatgaacc tggtagcaaa
     6181 agagtatgtt gctgctcagg acccagccaa cccgggcgtt cttgttcttt cgcaatttgc
     6241 gggagcggca aacgagttaa cgtcggcgtt aattgttaac ccctacgatc gtgacgaagt
     6301 tgcagctgcg ctggatcgtg cattgactat gtcgctggcg gaacgtattt cccgtcatgc
     6361 agaaatgctg gacgttatcg tgaaaaacga tattaaccac tggcaggagt gcttcattag
     6421 cgacctaaag cagatagttc cgcgaagcgc ggaaagccag cagcgcgata aagttgctac
     6481 ctttccaaag cttgcgtagg agcggttaat cacccgtaag tggaactaac agcacatcga
     6541 cctcacttga ggcaatcact cttttcgccg agcaggatgc tcgcgaaaag aaactgtgat
     6601 tgtgattacc gcaaatcacc aaatcgaaat gatgtttgtg acatacttcc agaatatgtt
     6661 cgcttaactc accgtaggca ataaacgtct tgtcaacggg ataccccgca tcctgaatta
     6721 acttatcaag aaagctttgc gtttcttctt gcatcacgct gcgtaaatct tcaagcatcg
     6781 gcgcggctaa ttgattgtac atttccgggt cggaagcgag agtaattaaa ctgatgtgtc
     6841 cctttactgg cctggcgata gataccgctt ttgccagcag ttgctgactt tccggtgtaa
     6901 ccgcaacagc gacaagaata ttgctatagc tcataacctg ttccttattc tgtgaacttc
     6961 aggtgacatt aaagctttac tcctaatgga gaaacgacgc aatcccaact cggtcaaaca
     7021 ggtaaatatt agtctgatta tt
//
LOCUS       DAPHDX010000184         7037 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPHDX010000184 DAPHDX010000000
VERSION     DAPHDX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.187"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..927
                     /locus_tag="RH058_004086"
     CDS             22..927
                     /locus_tag="RH058_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3139593.1"
                     /translation="MLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDID
                     AQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYAK
                     AYALFNQAAQHGGNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATC
                     RKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSI
                     RRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTT
                     IVAATATINGKDVELLKAGAIEQKW"
     gene            complement(983..1525)
                     /locus_tag="RH058_004087"
     CDS             complement(983..1525)
                     /locus_tag="RH058_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3139594.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1509..1820)
                     /locus_tag="RH058_004088"
     CDS             complement(1509..1820)
                     /locus_tag="RH058_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV3139595.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2005..2895)
                     /gene="cyoE"
                     /locus_tag="RH058_004089"
     CDS             complement(2005..2895)
                     /gene="cyoE"
                     /locus_tag="RH058_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV3139596.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2907..3236)
                     /locus_tag="RH058_004090"
     CDS             complement(2907..3236)
                     /locus_tag="RH058_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV3139597.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3236..3850)
                     /locus_tag="RH058_004091"
     CDS             complement(3236..3850)
                     /locus_tag="RH058_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV3139598.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3840..5831)
                     /gene="cyoB"
                     /locus_tag="RH058_004092"
     CDS             complement(3840..5831)
                     /gene="cyoB"
                     /locus_tag="RH058_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV3139599.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5853..6800)
                     /gene="cyoA"
                     /locus_tag="RH058_004093"
     CDS             complement(5853..6800)
                     /gene="cyoA"
                     /locus_tag="RH058_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV3139600.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1915 a         1758 c         1754 g         1610 t
ORIGIN      
        1 aaaagaatat tattttctgt aatgctgata gtttcaccaa gcgtggtggc cagcgagaaa
       61 gcgcatgaat tgtatgacag catatacggt ggtaaacctg ctcccgatgt tataaataca
      121 ctgcataaaa tggctgaatc gggagatatc gacgcgcaga gcctgttagg ctgggaatat
      181 tatcagcctc gttatgatac caaacctgat gttcaggaag cgattaaatg gttcgagtta
      241 gcggctaaac aaggtgacag agaagctccg ttagcgctgg ggggtatcta ctacgacggt
      301 gagcaggtgc gggtggatta tgccaaagcg tatgcactat ttaatcaggc cgcgcagcac
      361 ggtgggaatt tagcatggtc caggttgggc attatgtacg ccaatggtca gtatgttgag
      421 gtagattgta agaaagcgaa ggaatatctt gataaaggtg tccacattta tggtggccca
      481 gaagactttc tggctacttg tcgaaaagac atgattgaca gaaaaacggt tgacgatacg
      541 ttacccgtga ttacggttac ccgttctggg atgcgagata attttttaga taaaggtttt
      601 tcctgcatgg atagcctctt cgccaccacc aataaattag gcgaagtggc caatcttcgc
      661 gtgacattca gcattcgtcg cccatccggt aaggagatta accaaacggt tggcttcgcg
      721 cctttcggtt taaaccggct gaatattagc tttactgatt acctatttgg ttcatttacc
      781 agtaattcgt ctcttattct ttataaaccg gaatttgagc gaaaatcttg tgcaactgta
      841 aggaccacaa tcgttgccgc aacggcaacc attaacggca aggatgtgga gttgctgaaa
      901 gcgggggcaa ttgaacaaaa gtggtaacgc tgctcagccc ccggctgagg caaaacttgc
      961 cctggcgggt ggcgataata atctaagccg cgagaatatg ctgaatcgcc aaaaacatag
     1021 gtacagcacc aaaagcattt ggtgtcgctg aaagctgaac aaagccgaga cgagcataaa
     1081 aattaatggc ggcagggtca gcatcaagat aaaccccttt aattggtaat gcctggtgaa
     1141 ttatttttac atgttcaaaa aaatcacata gtaggtcctg accaaagccc cgcttttgat
     1201 acttccgtgc tacccccaac atgactaatc tgacaacacc aatttctgaa ggttgtgaac
     1261 cctgaaggac gccagaaacg cgttgttttt ccagcgaata tgccgtaaaa gtacagatgc
     1321 cgatcagttc accactttgt ctgtcaataa gtgctttagc cgcacagtcg ctattacgca
     1381 cacttttctt tagcgatgcg cgtacaaatt tatcgataac agggttaccg caatcaaact
     1441 gtttttgccc aggataagta atatcagact gataattaca ggagaggact atgggcagag
     1501 taatctcttc atgcttgtcg accatcgctg tttttccttt tcattaacgc cttgagggcg
     1561 agcgtcggtt gagcaggttc agcgatgagt tggttcatca gttcccagct ttgattcgaa
     1621 agctcacggc gacgttggtt atcaagcaca ctttcggcgc gttccatagc agcgctcaaa
     1681 ataaaagcgc ttaaatctac cccaacggct tgcgcagctt cacgaagttt gtttttggcg
     1741 tcgggactag ttttgagttc gacccgcgca tttttcagcg ttttatcgac atcgggtaga
     1801 gtggctgttt ttccaggcat tatgacctct ttgattaact gaatgaaatc gtagggagtc
     1861 gatctcatac ataatacggc tatcaaccgt atgattaatt tatgttcatc tgctcactct
     1921 gtcaagatat acggttatta gccgtatctc tgatctcaga caataaaaaa ggtgccgtag
     1981 caccttttta atagagaggt tttgttacca cacagcagcc agcagcgtat gcgagtccgg
     2041 taccataaaa tcaacggaca tcatcaccga gagggcagtg atggcgatga tagagaagcc
     2101 gaacagcttg cgcgcccaga ttctgtcatc agcaacttta taaccgcgca gagccatacc
     2161 taaccaccag acgctaaccg ccgcggcgac caccagatat ttatatccag cgtaaccgcc
     2221 aagagagagc atcagcgtgg caacggcaaa ggcgatgata tacagcgtga tgtgattctt
     2281 cgccaccgaa atgcctttta ccactggcaa taccggaatg tttgccgcct ggtaatcctt
     2341 aaagcggaaa atggcgatgg cataggagtg aggcatctgc cacaggctga agatagccag
     2401 caggatcgct gcgccgctat cgaactcgcc ggttactgca cagtagccaa tcaccggcgg
     2461 cgcagcgccg gagagcgaac caatcaatgt gccgtagact gagtggcgtt tcatgtacag
     2521 gctatagacg ccgacataaa ccacaaagcc catcaccccc agccagcagg ccagcggatt
     2581 cgcgccaaac cacagcagca taaagccagc aatacccagc aaggtggcgt acaccagcga
     2641 gacagcagga gagatcaggc ctttaaccag cacccgattc ttcgtccttt ccatctttct
     2701 gtcgatatcc ctgtcgatgt agttgttaaa cacacaaccc gacgccacaa ccagtgacac
     2761 cccaaccagc gtgtagataa acaggggata atcaatgctg ccctttgagg ccagcaggaa
     2821 tcccccaatc accgagatca ggttgccaaa gatgatgcct ggtttcgtta cttgcaggta
     2881 ttgcttaaac atcataaccg ccgctcttag tgcatcatca tgttgtagtt gaggttccac
     2941 ataatccaga tggagcctac aaccaggata gcgatgatta gcacggtgaa gacaaacgcc
     3001 gtcatgttcc agccttcatc tgatttggta ttcatgtgca ggaagcacac cagatgcacc
     3061 agaacctgta ccactgccat tgccaggatt gttcccagaa ttacggccgg agaggcagcc
     3121 cctgtcatca ccatccagaa cggaatcacc gtcagaatga tcgacaggat aaagcctgtc
     3181 atgtaggttt ttacgctgcc atgggacgcg ccgctgtgat cggtagaatg actcattaca
     3241 tcgcccccat cagataaaca acagtgaaca cacagatcca aaccacatcc aggaagtgcc
     3301 agaacaggct caggcacatg atgcgggtac ggttagtgct ggtcaggccg cgacgagcga
     3361 tttgtaccat cagcaccgcc atccagataa gaccggaagt gacgtgcaga ccgtgcgtgc
     3421 cgaccagcgc aaagaacgct gacaggaagc cgctgcgatc cggacccatg ccgttaacaa
     3481 tcaggtgatg gaattcgtag atttccatcc cgataaatcc ggcaccaaac aaccaggtca
     3541 acgccagcca ggagataacc tggcttttgt tgtttttgta catggcgata gccgccatgc
     3601 cgtaggtgat ggagctgaac aacagcaaga aagtttcaac cagaacgaac ggcagttcga
     3661 aaatgtcctt acctgtcggg ccgcctgcgg tgccgttcac cagaacggca taggtagcaa
     3721 acaagattga gaacagaatg cagtcgctca tcaggtagat ccaaaatccg aagattttgg
     3781 ttccgcctgc atcgtggtgc ccgtgttcgt gcgcgtgggc agtcgcgtgc gtcaaagtat
     3841 cagttgccat ttttcagccc tgccttagta atctcatcga aatgctggtt ttccagtttt
     3901 tcgatttctg ccaccggcac gtagtaatcc acgtcctcgt cgaagctttt cacgatccag
     3961 gtgatgatca tgcctgcgaa gccaacaatc gccagccacc agatatgcca gatcatggcg
     4021 aaaccgaaga tggtggagaa agctgcaatg acgatacctg caccgctgtt tttcggcata
     4081 tgaatttctt catagtggtc aggcttttta tacgcttcgc ctttctcttt catttcccag
     4141 aatgcatcac gctcgtgaac gtgcggcaca acggcaaagt tatagaacgg aggcggggaa
     4201 gaggttgccc actccagcgt acggccaccc cacgggtcgc cagtcaggtc acggttctgg
     4261 tcgcggtcgc gaatagaaac gtacatctga ataacgaggc agagaatacc cagcgcaatc
     4321 agtactgcac cgctagctgc aatcatcagc atggtgtgga actgcgggtc aatctgctgg
     4381 ctcaaacgac gggtcatgcc catgaagccc agcgcataca gcggcataaa ggcaacgaag
     4441 aagccgatga tccagaacca gaacgcgcgt ttaccccagg tttcattcag tttgaaaccg
     4501 aaggctttag gccaccagta ggtcatccct gcgaagcagc cgaagaccac gccgccgatg
     4561 atcacgttat ggaagtgcgc aatcaggaac aggctgttat gcagaacgaa gtccgcgccc
     4621 ggtactgcca gcagcacgcc agtcatcccg cccaccgaga aagtgacgat aaaaccgatg
     4681 gtccacagca tcgcagaatg gaacacgatg cggccctgat acatggtgaa cagccagttg
     4741 aagatcttca ccccggtcgg gatggcgata atcattgtgg tgataccaaa gaaggcgttt
     4801 acgttcgcgc ccgcacccat cgtaaagaag tggtgcagcc aaacgatgaa cgacagcacg
     4861 gtgatacaga cggttgccca taccagcgag gtataaccaa acagacgttt acgcgagaag
     4921 gttgccgcaa tttcggagaa cacaccgaaa acaggcagga tcaggatgta aacttccggg
     4981 tggccccagg cccaaatcag gttgatgtac atcatcatgt tgccacccat atcgttggta
     5041 aagaaatggg tgcccagata gcgatccagg gtcaacaacg cgacggtaac cgtcagaatt
     5101 gggaaggaag caataatcag tacgttcgcg cacagtgatg cccaggtaaa tactggcatc
     5161 ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg taacgaagaa gttgataccg
     5221 gtaagcgtcg tacctatacc ggatagctgg agactccata tccagtaatc gacaccgact
     5281 cccggactgt actctattcc cgatagcggt ggataggcca gccagccggt ctgcgcaaat
     5341 tcgcccacgc cgagagaaac gttaaccaga atcacaccaa caacagtaaa ccagaagctt
     5401 aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga tctgcagcgg aaccaccagg
     5461 ttcatcagac cgataacgaa aggcatcgcc acgaagaaga tcataatcac gccgtgcgcg
     5521 gtgaagatct gatcgtagtg gtgaggtggc aggaagcccg cttcgcccgc cgaggcaaga
     5581 gcctgctggc tacgcatcat aatggcgtca gcaaaaccac gcagcaacat cacaatcgcc
     5641 acgatgatat acatgatacc gaggcgttta tggtcgacgg aggtcagcca ctctttccac
     5701 aggtaggtcc acttaccgaa gtaagtgatc aggccaacga gcgccagacc tcccaaaata
     5761 atgccagcga tcgtaaccat gacgataggt tcatggaacg ggactgcatc aagtgataat
     5821 tttccgaaca tctttattct tcctcaaccc ctttaatggg cggattccgc gtggctcatg
     5881 tccatgcctt ccataccttc gtgtgcactg tgctcacctt ctggctgggt catgtccatg
     5941 ctcttaccgt gagccataaa cttgttaatc acatcggcaa acaagtctgg tttcacgttg
     6001 gagaaatatt ccacctggtt gtattcgcta ggcgcggcca gtttttcgaa cgcagccatg
     6061 tcagacatgg tgttcggcga ctgtttcgct tttgcgaccc attggtcgaa ttcggcgcga
     6121 tccggtgttg caatagcttt gaacttcatg cctgagaagc ccggcccgct gtagctggcg
     6181 gagataccgt cataagtacc gggttcgttg gcgatcagat gcaggcgagt ctgcataccg
     6241 gccatggcat aaatctggct acccagacgc ggaatgaaga aggagttcat cacggagttg
     6301 gaggtcactt tgaagtacac cggagtgttc gcagggaaag cgatttcatt cacggtagca
     6361 atgccctgtt ccgggtagat gaagaaccat ttccagtcca tggaaaccac ttcgatggta
     6421 atgggcttct cgtcgtgtgc cagcggcttg ctaggctcaa gagcgtgagt ggttttccag
     6481 gtcagtaccg caaggaagat gatgattaag ataggtaccg tccagaccac agcttccact
     6541 ttattggagt gtgaccagtt cgggctgtac ttagcatctt tattgctcgc acgatacttc
     6601 caggcgaaac caacagccat caagattgcg ggaataacga caatcaacat caggccaaat
     6661 gccgtcagta tcagtgaacg ttgctccaga ccaatctgtc ctttgggatc taacagcgca
     6721 gaattacagc cactgagcaa tacagtgcct gcaaataatg acaaccatcc caaactttta
     6781 ttgtatttcc tgagtctcat ttaacgacct caattccacg ggatctggtg gcgtttaaag
     6841 tgtgtgggca ttttacggga aggttacatt actgtaaaca tgattaaatt cctgttactt
     6901 ggatttggct gtcaggtcac atatgttgca aaacatatca agttgatttt aagaagctgt
     6961 tgcaaaaggg cgttataacg aaaggggagt gcagaaggag tttggacggg caattggtat
     7021 aaccaatctg gaataaa
//
LOCUS       DAPHDX010000185         6986 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPHDX010000185 DAPHDX010000000
VERSION     DAPHDX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.188"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(155..1804)
                     /gene="pgi"
                     /locus_tag="RH058_004094"
     CDS             complement(155..1804)
                     /gene="pgi"
                     /locus_tag="RH058_004094"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV3139601.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2329..3678
                     /gene="lysC"
                     /locus_tag="RH058_004095"
     CDS             2329..3678
                     /gene="lysC"
                     /locus_tag="RH058_004095"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV3139602.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3823..4749
                     /locus_tag="RH058_004096"
     CDS             3823..4749
                     /locus_tag="RH058_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDV3139603.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4785..5576)
                     /locus_tag="RH058_004097"
     CDS             complement(4785..5576)
                     /locus_tag="RH058_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDV3139604.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYGVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEPPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPMWFSPLRARWHYYSMAQKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5582..6709)
                     /locus_tag="RH058_004098"
     CDS             complement(5582..6709)
                     /locus_tag="RH058_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDV3139605.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILLEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1721 a         1818 c         1674 g         1773 t
ORIGIN      
        1 gtaattaatc tgagtatttt ccgaaatcaa tcacgattaa aaatctattt taggattaac
       61 ctcacggtat gatttccgtt aaatcacaga caaggggatt tatctgataa aacaatgccg
      121 acataacgtc ggcattgtta ttaaggtttt taaattaacc gcgccacgct ttatagcggt
      181 taatcagacc attggtcgag ctatcgtggc tgctgatttc tttatcatct ttcagctctg
      241 gcagaatacg gtttgccagt tgtttaccca gctccacgcc ccactggtcg aaggtgaaga
      301 tgttcaggat cacgccctga gtaaagattt tgtgctcata cagcgcaatc aacgccccca
      361 ggctgaacgg agtgatttcg cgcagcagga tggagttggt cgggcggtta ccttcgaaca
      421 ctttgaacgg caccacgtag tcaagcgttg ccggatcttt accctgatcg cgatattcct
      481 gctcaaccac ttcgcgggat ttaccaaacg ccagcgcttc ggtctgggcg aagaagttag
      541 acagcagttt ctggtggtga tcagagagcg ggttatgggt gatagccggg gcgatgaaat
      601 cgcacggtac cattttggtt ccctggtgga tcagctggta gaacgcgtgc tgaccgttag
      661 tgcctggttc accccagata atcgggccag tctggtaatc cacaacgtta ccgttacggt
      721 caacatactt accgttggac tccatattgc cctgctggaa gtacgccgcg aaacggtgca
      781 tatactggtc atacggcaga atcgcttcag tttccgcacc aaagaaattg ttgtaccaga
      841 tgccaatcag cgccagcagg acaggcaggt ttttctcggc aggcgtggtg gagaaatgct
      901 tgtccattgc gtgtgcgcca gaaagcagtt caacgaagtt atcaaagccg atggagagaa
      961 caatcgacag gccaatcgct gaccacaaag agtaacggcc gccgacccag tcccagaact
     1021 cgaacatgtt ggcagtatca ataccaaact cgccaacggc tttggcattg gtggaaagcg
     1081 ccgcaaagtg ttttgcaacg tgtttttcat cacctgccgc tttcaggaac cagtcacgcg
     1141 cgctatgggc gttggtcata gtttcctgag tggtgaaggt tttagatgct accaggaaca
     1201 gcgtggtttc cgggtttact tttttcagca cttcagcgat gtgagtccca tcgacgttag
     1261 aaacaaagtg catgttcagg tgatttttgt acggacgcag tgcttcggtc accatgtacg
     1321 ggccgaggtc agaaccaccg atcccgatgt tcactacgtc agtgattgct ttgccggtat
     1381 aacccttcca ctcaccggag ataatcgctt ccgagaaggt tttcatcttc tccagcaccg
     1441 cgttgacttc cggcattacg tctttgccat caaccagaat cggggtattg ctacggttac
     1501 gcagcgctac gtgcagcacg gcgcggtttt cagtgcggtt gatcttctcg ccagagaaca
     1561 tcgacttaat cgcgcccgcc agatcgcact ctttcgccag atcctgtaat ttcgccagcg
     1621 tctcttcagt gatgcggttt ttggagtaat ccaccagcat ctgatcgtcg aaggttgcgg
     1681 agaacttaga aaaacgatcg ccgtctttag caaaaagatc ggcgatcgta acgtctttca
     1741 tttcatcgaa gtgtttctgt agtgcctgcc aggcagcggt ctgcgttgga ttgatgtttt
     1801 tcattagcaa tactcttctg attttgagaa ttgtgacttt ggaagattgt agcgccagtc
     1861 acagaaaaat gtgatggttt tagtgccgtt agcgtaatgt tgagtgtaaa cccttagcgc
     1921 agtgaagcat ttattagctg aactactgac cgccaggagt ggatgaaaaa tccgcatgac
     1981 cccgtcgttg acaaccgccc cgctcaccct ttatttataa atgtactacc tgcgctagcg
     2041 caggccagaa gaggcgcgtt gcccaagtaa cggtgttgga ggagccagtc ctgtgataac
     2101 acctgagggg gtgcatcgcc gaggtgattg aacggctggc cacgttcatc atcggctaca
     2161 ggggctgaat cccctgggtt gtcaccagaa gcgttcgcag tcgggcgttt cgcaagtggt
     2221 ggagcacttc tgggtgaaaa tagtagcgaa gtatcgctct gcgcccaccc gtcttccgct
     2281 cttcccttgt gccaaggctg aaaatggatc ccctgacacg aggtagttat gtctgaaatt
     2341 gttgtctcca aatttggcgg taccagcgta gctgattttg acgccatgaa ccgcagcgct
     2401 gatattgtgc tttctgatgc caacgtgcgt ttagtagtcc tctcggcttc tgctggtatc
     2461 actaatctgc tggtcgcttt agcggaaggg ctggaacctg gcgagcgatt cgaaaaactc
     2521 gacgctattc gcaacatcca gtttgccatt ctggaacgtc tgcgttaccc gaacgttatc
     2581 cgtgaagaga ttgaacgtct gctggagaac attactgttc tggcagaagc ggcggcgctg
     2641 gcaacgtctc cggcgctgac agatgagctg gtcagccacg gcgagctgat gtcgaccctg
     2701 ctgtttgtcg aaatcctgcg cgaacgcgat gttcaggcac agtggtttga tgtacgtaaa
     2761 gtgatgcgta ccaacgaccg atttggtcgt gcagagccag atgtagccgc gctggcggaa
     2821 ctggccgcgc tgcagctgct cccacgcctc aatgacggct tagtgatcac ccagggattt
     2881 atcggtagcg aaaataaagg tcgtacaacg acgcttggcc gtggaggcag cgattatacg
     2941 gcagccttgc tggcggaggc tttacacgca tctcgtgttg atatctggac cgacgtcccg
     3001 ggcatctaca ccaccgatcc acgcgtagtt tccgcagcaa aacgcattga tgaaatcgcg
     3061 tttgccgaag cggcagagat ggcaactttt ggtgcaaaag tactgcatcc ggcaacgttg
     3121 ctacccgcag tacgcagcga tatcccggtc tttgtcggct ccagcaaaga cccacgcgca
     3181 ggtggtacgc tggtgtgcaa taaaactgaa aatccgccgc tgttccgcgc gctggcgctt
     3241 cgtcgcaatc agactctgct cactttgcac agcctgaata tgctgcattc tcgcggcttc
     3301 ctcgcggaag ttttcggcat cctcgcgcgg cataatattt cggtagactt aatcaccacg
     3361 tcagaagtga gcgtggcatt aacccttgat accaccggtt caacctccac tggcgatacg
     3421 ttgctgacgc aatctctgct gatggagctt tccgcactgt gccgggtgga agtagaagaa
     3481 ggtctggcgc tggtcgcgtt gattggcaat gacctgtcaa aagcctgcgg cgttggcaaa
     3541 gaggtattcg gcgtactgga accgttcaac attcgcatga tttgttacgg cgcatccagc
     3601 cataacctgt gcttcctggt gcccggcgaa gatgccgagc aggtggtgca gaagctgcat
     3661 tttaatttgt ttgagtaata tttccctact ggcgtataac gctcaccgat tgttatacgc
     3721 catcctcact gaaaacatag attaaataat atctgaaaag caaaatcact cctgtagaac
     3781 agtattataa tcaccgaaaa atatcattta acaggagtgt taatggctac tttaatggaa
     3841 aaagatgcac ttttaaacgg agccagtcag tgcatcgcgt ttttatcaaa tatcattgat
     3901 aattgctcag tttcgtcgca tcaggattca ggcgatgctc tcaagcgatt agtcagctat
     3961 cgcgattatc tttattctac ccccgctgaa cttgttgatt ttacacaagg taagattctc
     4021 cttcagcagg taagaactca atatcagcat gaattcaaca atactacgca ttcagaaaat
     4081 aaagcatctt ttgattctat ttggcagcgt ctgacaaatc atgaagtcac accgcaacag
     4141 catcccattg gttttgtttt aggtgggcaa cccggagcag gtaaatcatc tctgattgaa
     4201 ttagccaaaa gagagacaaa agataatata atgattatta acggtgatga ttttcgattt
     4261 ctacaccctg attttaatta tatctaccaa aattatgggg atgattttgt tacccataca
     4321 gcgaaatttt ccggcgaaac ggtcgaacgt gcaattgaga gagcgatagt cagtaaactc
     4381 aacattgttg tcgaaggaac atttcgtaat gccgcgacgc cgttacaaac attaaaaaaa
     4441 ctcaaagatg caggctacca aacagaagtc atgataaaaa caacctcagc ggcattaagc
     4501 tgggaaagca ccaatgaacg gtatagtaag gacaaagagg cgggaaatat tgcccgaaaa
     4561 gtggataaaa accaccatga tatcgtcacc ggtttgctgg cagaaaatgc ccgcaaggtg
     4621 tttgcttcga acctgtcaga taagtttgcg gtgtattcgc gtgagaagat gatatttagt
     4681 tcacaagcgg caacgaatga cgatattgca acacttatac agaatgaaat cagcgggaat
     4741 acacaataat aataagagct gatggcgtga aataacgccg ggctttatgc ccggcgcatt
     4801 accgtctggt acattaagct atccagttcc ttaacgtccc cttctctgct atccggtaac
     4861 agcggtggaa tttccccacg cgcctgttcc gcacgaataa acgcatgtaa caatggacgg
     4921 cgcggaccat attccgcttc tcctgcgcgc tgtttacgtt ccagtaattc gtctatttct
     4981 gcacgtaacg gcgcatcaag ttcgcttccc gccagtaact cagcaaagcg cattggcggc
     5041 acgccttttc ccgcttcgac ccagcgcacg gccagcagcg gacgcagcac gtaaaagtat
     5101 tttttcagtc gaacttcatc gccctgcaaa tatccgcgga agtttttctg cgccatcgaa
     5161 tagtaatgcc agcgcgcacg cagcggagaa aaccacattg ggaccattgc cttaagcgcc
     5221 gttatcgttt cttcatcttg ttgatagacc accggagaat ccagccattc tatcagcgtc
     5281 gggttcgcgc ctttcaataa tcccagcgcc ttacgccact cccaaccgga aacatctaac
     5341 tcgtcgtcga tgggcagctc gataacatcg cgcggcggct ccacccgcag ataccattcc
     5401 agcgggtgaa catacaaaaa ccgtacatcg tagtcgctgt cgggcgaggc aaacccccag
     5461 ccgcggctcc cggattcaca ggcatacagc actttcacgc cgtagcgccg ttctatttct
     5521 tttaactgct gcgatacccg ttcgcgcatc gcagcactga ctccatttaa tggcatgtta
     5581 ctcacccttt gatattaacc aacccatgca gaacctgatc cagaccgcca aatacagaga
     5641 tgttatcgat ccgctcagcc actctctcca gcgtttctag ctcttttaaa cgcaacgcta
     5701 ccggattgtt ctccatgact ttggcggtgt tcagtaacga gcgcgtcgct gccgtttctt
     5761 cacgtcggcg aatcacattc gcctgcgcag atttctccgc ctcaaccagc tgcgcaagga
     5821 tatttttcat atcgccaggc aggacgatat ctttcacccc taatgaagca atctccatac
     5881 caaatggcaa catgcgcgat ttcacctgtt cgctgacaac gtcatcaatg acctgtttat
     5941 cttccagcag ttcatccagc gtacgagtac ctaccgcttc acgcagggca aattgcaatt
     6001 cccgatacaa atgatcaatc ggcttcgtta actgactaaa tgccagcaac acgtcgctgt
     6061 agcgccagtt agcggcgaga ttaatccgca gattgacctt atctttcgtt aagatttctt
     6121 gcccgctgac ttccagtacc tgcaagcggg tgtcgacaac ttcagcctca accagatgat
     6181 ttattttcca gtatgctgtc agccccggcg gtaacagcgc ctgcgtttca ccatcgattt
     6241 tcagcacgcc tacatgccag gccggaacct gcaccgtgag tatcgcatcg cggcctttaa
     6301 ctgccccttt gcgccgtggt tgcaataccg cattcatcac ctccgtttgt acctggactt
     6361 gctgcgtatt catccgcacc agtgtcaaat catcttctac acgccagtac aaacggcgcg
     6421 ttgatggcgg cagaatctcc agcaaaatac cgtccatata caacgccccg gcttcaatct
     6481 ccgacaagtc tgccaccagg caatatttct ccacccaatc aggctgaaag cggcgcaaat
     6541 aatccgccag cacgtcggga acttcgctgc catcgagcgt aatcagcaag acttctggcg
     6601 tatttaacca gggtaaaaga tgctcgcctg catgcagcac tttgtagtaa tcgccatttc
     6661 tggagagtaa tgccagctga tcttttctta cgcttatttt cttaatcatt atctattcct
     6721 tgcttaacag ccgtcgcgga agcagacacg aagcgacagg ggaattccct tttgcctgtt
     6781 acccggcgtc tttatcggcg acgttatcgg ggatcgctga ctgcgcaatc cctcactttt
     6841 tatttaataa gttacaacca gtattcattt caggaatgaa tgtgggaatc gaacccataa
     6901 ttggcgtcaa aggctgacgg aacacgcccg gaaaacagcc tggccgttcc tggtgtgccg
     6961 acggggtttg accccctttg taattc
//
LOCUS       DAPHDX010000186         6973 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPHDX010000186 DAPHDX010000000
VERSION     DAPHDX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.189"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2771)
                     /locus_tag="RH058_004099"
     CDS             complement(<1..2771)
                     /locus_tag="RH058_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV3139606.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGV"
     gene            complement(2828..5200)
                     /locus_tag="RH058_004100"
     CDS             complement(2828..5200)
                     /locus_tag="RH058_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV3139607.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5238..6923)
                     /locus_tag="RH058_004101"
     CDS             complement(5238..6923)
                     /locus_tag="RH058_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV3139608.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHEHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1805 a         1599 c         1569 g         2000 t
ORIGIN      
        1 aatactccgg tataagtatt taccggatga gaaagatatt gtttaacggc agtgttaaca
       61 ttctctaccg tcatttgttt caacaattgc tcctgctcag tccatgctgc aggatcgtca
      121 tattgaataa gactatttac aatagtgttc gctaattgtt gaacgctacg ctgttggata
      181 tcgaggctgc gctgaacgtt ttgctggtat tcattcagtt cttgctcact gatcccttta
      241 gccagacgct taaccatcac ttcattcgct aacgttaaca gttcatcatg tcgttctggt
      301 tgacaagtaa aagccagcaa atgactgata tctttggcct gaggatcaac cgagaggcga
      361 gaagaaacgc tgtatgctcc agatgcctgt tcacgaatat taacacgtag atcttttgcc
      421 agtgcgacgt taaaagcatc gagcgccata cgcgtcgcca gattaacagg tgtccgggaa
      481 tcataacgct tccactgtga aaactgtgcc acaggttcat tttgttcttt tacagtaacc
      541 gatgcgttgt ccgtcgcgcg agttaatggt ttacctgcgg ctaatggcga atcagagtgt
      601 ttgattgatc ctaagtaacg cgtaattaac gccacgagtt tgtcttctga gacattaccg
      661 acaatgacaa acgtgatatc cgctggagat gaaaacaact ggcgatcggc agccagcgca
      721 tctgcggcag taaactgtac aatctgattt tcttgcagta atttcgtgcg gtcatcagca
      781 tagcgcgtct catacatctg ctgggcgaat ttctccgctg gacgctggtc gagcgttttc
      841 aacgcctgaa tttgagcatt ttgtagcgat gcccaaatat tgtcgttaat cgtgctgtgg
      901 gtgattcgct ggttaatcaa ctgaaaacca ggttcagggt tattagtccg cgcgctaacg
      961 gagagcaacg tattcatgcc actgacttta ctgctcatgg ttaccgaatt ttccgcactc
     1021 cagcgtttca ggctggaaga ggagagctca ccaacgccgc ttccgcttac tgctttattt
     1081 gccagcgcga taagtgattt ttgctgcgca gggaaactta aatcgccttt attcgatacg
     1141 gcagtaattt gtagcttttg ctcttcaccc gcggattttg ccagaataac cctggcacca
     1201 ttggaaagtg ttaatgatgt cagattctca gccagcgttt ctttgctgct aatttccgcc
     1261 tgtgggtcag cgtctactgt taacgataaa tttctgcctg ggaagatgta agccgccagc
     1321 tttttgttgg cgtactcttt ttccagcgcc aggatagccg caggagacaa tgcttttttg
     1381 gcggcaagct cattgtttac catttgctcc caaaatgcgt cctggttctt tcttaactgc
     1441 tgccattttt ccgccagact ttgcacggta atttgctgcc acagacgttt cgaaagttga
     1501 tatgtctctt ccggcgacaa gaacggcgta ttatttaatg agctggatgc caggcgactg
     1561 gtcagcatac gtaaatcacg ttcagcttgc tgatcaaccg catttttcag ccaggtgagg
     1621 cgggtagatt tgacatcatc gagttcttca gcagaaaaac catgctgatc aatggttgcc
     1681 aactctgcca ttaatgcatt cgcagcatcc tgcatattat cgtctcgtgc atttacacgg
     1741 aaaaacagcg actgataatc gggtgcaatt ttaacgctgc gcgcagtgcc gccagaaata
     1801 gtcttcaact cgcccgactg tatgcgttcc tgcagacgtt gattgaacag ctgaactaac
     1861 atgctccatt cagcttgttc gataaagctt tgctcatcgt tcacttgtac cattggcagg
     1921 cgataataga gtgcgatgcc gttcacccgg ttttctttat cattaatgat attaaagcgc
     1981 aggtggtttt cggcttttgt cggccagacg cgattttcag ctgctttgtt agccggaagc
     2041 ttacttaaat tatcctttat cagcgccagc gcttctttac tgtcgatatc gccgaccacg
     2101 ataaaggtca tattatttgg ttgataccag cgttgataaa attggcgcaa ttgtgccggt
     2161 gtgaccgtgg cgacagtatc catcaggccg ataggttcac ggtctaaatt acgggtatta
     2221 gccagcagga aagggcggcg cgcctgagag gtgcgccatt tcgcatcctg atgggcacgc
     2281 cattcctcag taattacgcc acgttcagcg tctacttcga gtttttcaaa ggttgcggca
     2341 ttactccatt cactgaagat tgccatcact tgttgcagat tttgtttctg cgtagtcggc
     2401 aaactcacct gatacaccgt ttcgtcatag ctggtatagg cattaacatc gcgaccaaaa
     2461 cgcaggccca ttgactcaaa tgtttcgatg actttattac ccggccatgt ttttgtgccg
     2521 ttaaacatca tatgttctac aaaatgagcc acgccgcgct cattgtcttc ttcctgcaat
     2581 gaaccggtat gaatttgcag ccataaattt acctgatcct ttggatgagc atgcggataa
     2641 atcatatatc gcaagccatt gcccagttgc ccggtaatta acttttcatc ctgcggtaag
     2701 gcggcggcaa tcagccgccc ggggagcaac agagttgcca ctaacgtcag taagaaacag
     2761 aggtttctca taattatctc catgcgaaaa ccgggcgaat ttacccggtt aagtaaaatc
     2821 cgaactatta aaatttcatg ctgacatcaa gccagaaagt acggccactg gcgtaggtcg
     2881 ccacaccggt gttcgttgta tcaacagcgg ttttgctatc cagtacattg agaatatcgg
     2941 cactgatcgt caggttttgt tgtttcagaa attgtggcgt ccaggacaag cgggtgtccc
     3001 aggtcaggct gctatccagt ttttcgtcaa catactgctt gtaatctgaa tattcgctga
     3061 tgtattgcgc atttgtctta ccaaggataa tgcgagcttt acgcgcttct tgccaggcca
     3121 gcgtattcgc ccacactaac ccgctcggtt gatgcgtgaa atccatgttt aaggagatct
     3181 ttaatgggtt attaaaatct gccactggaa cgctatcgta agagaccaga ttaccgttat
     3241 aaaccacctg gttatcaccg gtattgctct cctcataacc attgtttaac gacaagttgc
     3301 ccttgctctt gatatagcta aagacaattt gtgggttaat atctacctgg cggatatgca
     3361 ggggttcggc cagttcaaaa ctgaggctga acgaatgcgt tttggttttg ccatcgttgt
     3421 tatattcagt aatggtggtt ttagtcgcgc tgtcggtacg actgcttttg ctgatttgat
     3481 catgcgcttc acggtaaaca tagtttgcgc gtgcaataac gttcttaccg attttttgct
     3541 gcaatcccat tgccagttca tcgttataag gcgttttcaa attctgataa cgcgtcaggg
     3601 ttttattacc tgataccgat tccgtccagc tattgcggat atcacgtaat cccatatcaa
     3661 gaatattccc gccatagtaa cggttataac cggcggtaat cattgaggtt tgatcagcaa
     3721 aaatatccca ttccgtcata aagcgcgggg agatattgtg gtttgacaga tagttgtcat
     3781 agtcgtaacg cacaccgggc attaacgaca cattccgcca gctaatgtga tccgccatat
     3841 acagcgtgta gttgtcaatt cccaggctgc ctttaccttt atggtaaatg gtatggttag
     3901 ttttctttcc tgcagcatta atcacatagg attcagactg gttatggcgt tcagtccacg
     3961 catcggaata gatgtattcc gcgccgaagt agggttgatg cgaaacatta cccacggcga
     4021 atttttgcca gtccaggcgt gttttgaagg tgtaattatc tacagcctgg gaaatgtgtc
     4081 ctaatccgcc acgggtgcaa cgccctgtaa tatcaccata tgtacatgaa agttcggtgt
     4141 accagatgtc atgatcgtga cgggtataat cactaatatg atcccaacca acggtggtac
     4201 gcagtttggc ccatgcgagc tgcgtatcca tatcccatgc cagaccgtag gatttattac
     4261 ccatttcgcg atcagactgc gggaaggtgc tggtattata atcacggctg gagccggtat
     4321 attttaaggt taaatcgtgg gtaaagcggt cgctggcaaa ccaggtaaat ttgctcaatg
     4381 cagtatcgat aacgttttta tactgtgccc gaccggcgac aatgccgtca ttcgaaacat
     4441 aatccgcgcg ggtgatatca gactggcggc gcgataaacc ggcggtaacg ccgaagttat
     4501 cagcgagttc ctgattaaac gacaaggtat aaaagttctt tttaaaatct ggggagtaat
     4561 aagtacttcc tgaagagcct tgattaaatg cgctcttgtt attctcatcg atatgcgatg
     4621 ttaaccagtc cgaacgcgta gtgcgataac ccaatttaac cttgctatca tcagcgttga
     4681 agcgtttgat ctttgcatca attaccccgc cattgaagcg accaaattca accggcacaa
     4741 aactgtcata aagcgtcaca ttgtccagta agctgacatc aagataatac ccctgtgaca
     4801 tcccgctaat attggttgca ctactggcat cggactcatt cgctgggttc aggttattag
     4861 ttgcactaat accgtcaatc aaataggcat tctggtaggg cgacgcaccg tgaatagaga
     4921 ttttctcagg gcgaatatct ccctggttca acgaggtact ttgcgttgaa tccatgcgta
     4981 ccgcagggtt ggttctcagc agatcgctga tattgccatt cccggtcggt aaacgctcga
     5041 tgctttcact ggtgtaatgc gtgttgccgt ttacgggtac agggacagga gcatagaccg
     5101 tcatctcttc aaaaaaatgc atgtacatgt ttttgtcatc gacggcctgc gccacatcag
     5161 cgccacataa aatgacgcca ggaataagaa ctcgcttcat ttatttatat ctccgtacag
     5221 cagggctttt attgttgtta taaaaccgtg ctaatgtcac aaatatcatc agccaggttc
     5281 cagacaccgg gttgatgtgt aaccataatg acgccgcttg tgggtagttt ttcacgcact
     5341 aaacgcagta agcgtatagc ctcttgttcc tcaagatgag aggtagtttc gtcaagaaat
     5401 atccatttcg gacgtcgtaa aattaatcgt gccaaggcga tacgttgttt ttcgccgctg
     5461 gaaagaatat ctccccagcg atcgtgctca tgaatacgcg cagccaattt ccccagacca
     5521 acctgatgca gtacttcgct caacgattta tcgtctacgg gcaggggaag tgctttacaa
     5581 ataatctctt tcagtaagcc gcttttgatt aacggtgttt gtgacacata ccaactgtca
     5641 gcaggagaag aaatatcacc tttaaaccac ggccagcagt gggataatgt tttaagcagt
     5701 gtggtttttc ccgcaccaga gtagcctttc agtaatagcc atttgcctgg cgaaacatga
     5761 aagttcaagt tctctaatat gatcttatta tcaggcgtac gaatactcgc attagccact
     5821 tgtaccgcat gttggcaatt tttaggctta ttcgtagggc gctgttcagt gagttgatgg
     5881 aactcataca agcgatcgat aaccgcagcc agctcagcaa gttcgtcata tttataaata
     5941 aaccagctta aattgttcga tactagcata aatgcctggc gcgatttcat tagtccgccc
     6001 agattgatct gcccgctaat aaactgcggc aataacagaa agtagggaag aacgctaagc
     6061 gagcgcgaat aaatattttg ccagtaatca agccaccgct gacgattcat taaacgatgc
     6121 cagttctctt taatggtatg aaaattgtcg ctcaactcct ggcgttgtag agactcagca
     6181 ttgcttagcg caatcagttc tgcctgctta ttatgctgca caagattagt tcgaaatgtc
     6241 gcttcgctac gttgtttttc cacattaagc ggacgaatac gcttaccaac cttatgggta
     6301 aataaagttc caccgatcac aatgagcaca acggtataga ccatataccc ctggatattc
     6361 cattctgttc caccaacagt gaatgagagc gtacccgcgc tttgccaaag aataacggta
     6421 aaggtgatca gcatgctaag tgactggatg aagccaaatg aaagactgag cgttttgctg
     6481 attagtaaga gaatgtcttc agcgatacgt tggtcagggt tatctgtatt tttatgctcg
     6541 ccgtagatct gcgtgaagta gtagttttta tctgcaaacc agcgattaag gtagtaatct
     6601 gttaaccatt cgcgccagcg aatagtcagc agtttaatta accatgtttt gttcacggat
     6661 attaatacaa agatccccag cagggcaggg aaccaaagga caagctgcca aagcttatcg
     6721 gtttctttct ggcttagcgc attgaaaaaa tcattattcc agtcgtttaa ccagacctga
     6781 attttaacca cgccaaggat catggcgagg ataataatga ttaacagcac tgaagtttta
     6841 ttattcttac gcagccaaaa gggcttcaac agacagagat actttgctat caacatacaa
     6901 aacgtaatgg gaatggttat cattagcgaa aattgattgt gccatccaat gattatctat
     6961 ggcaatgatt tac
//
LOCUS       DAPHDX010000187         6958 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPHDX010000187 DAPHDX010000000
VERSION     DAPHDX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.190"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..519
                     /gene="ftnA"
                     /locus_tag="RH058_004102"
     CDS             22..519
                     /gene="ftnA"
                     /locus_tag="RH058_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV3139609.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(557..796)
                     /locus_tag="RH058_004103"
     CDS             complement(557..796)
                     /locus_tag="RH058_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV3139610.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            987..2198
                     /gene="tyrP"
                     /locus_tag="RH058_004104"
     CDS             987..2198
                     /gene="tyrP"
                     /locus_tag="RH058_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV3139611.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2260..2925)
                     /gene="yecA"
                     /locus_tag="RH058_004105"
     CDS             complement(2260..2925)
                     /gene="yecA"
                     /locus_tag="RH058_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV3139612.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3121..3207)
                     /locus_tag="RH058_004106"
     tRNA            complement(3121..3207)
                     /locus_tag="RH058_004106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3171..3173),aa:Leu,seq:taa)
     gene            complement(3220..3293)
                     /locus_tag="RH058_004107"
     tRNA            complement(3220..3293)
                     /locus_tag="RH058_004107"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3259..3261),aa:Cys,seq:gca)
     gene            complement(3348..3423)
                     /locus_tag="RH058_004108"
     tRNA            complement(3348..3423)
                     /locus_tag="RH058_004108"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3388..3390),aa:Gly,seq:gcc)
     gene            complement(3575..4123)
                     /gene="pgsA"
                     /locus_tag="RH058_004109"
     CDS             complement(3575..4123)
                     /gene="pgsA"
                     /locus_tag="RH058_004109"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV3139613.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4180..6012)
                     /gene="uvrC"
                     /locus_tag="RH058_004110"
     CDS             complement(4180..6012)
                     /gene="uvrC"
                     /locus_tag="RH058_004110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV3139614.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6009..6665)
                     /gene="uvrY"
                     /locus_tag="RH058_004111"
     CDS             complement(6009..6665)
                     /gene="uvrY"
                     /locus_tag="RH058_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV3139615.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1681 a         1803 c         1651 g         1823 t
ORIGIN      
        1 taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag
       61 atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat
      121 cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat
      181 atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat taataccgtt
      241 gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac
      301 gaacaattaa tcacccagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac
      361 tacccaacat ttaatttcct gcaatggtat gtttctgagc agcatgaaga agagaaactg
      421 ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt
      481 atcgacaaag aactctctac cctcgacaca caaaactaat gctctccggc ggcaggttta
      541 ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa aaaccgtctt
      601 ccgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc gcagtcatcc
      661 cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt
      721 ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac
      781 cgtgaataga gtccatcgtt cctcctcaaa aaaaagccta gcgtagcgat taccgcttat
      841 gaagactttg cgccagcgca ggactgaatg cttttattgt acatttatat ttacaccata
      901 tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag atatcactca
      961 taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt gtttttatcg
     1021 tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg gcaggtgttg
     1081 gttttagcgt cacgttaatc ttgttgattg ggctttgggc gttgatgtgc tacacggcgc
     1141 tattactgct ggaggtgtac cagcatgttc cggcagatac cggtcttggc acgctggcaa
     1201 aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt
     1261 atgctctgac tgcggcatac atcagcggtg ccggtgaatt gttggcctcc agcatcagcg
     1321 actggacagg tatttctatg tcggcaaccg ctggcgtgct gttgttcact tttgttgccg
     1381 gtggcgtggt ttgtgtcgga acttcactgg tcgatttatt taaccgtttt ctgttcagcg
     1441 ccaagattat ttttctggtg gtaatgctgg tactactgct gccgcatatt cacaaagtga
     1501 atcttttaac cctgccgttg caacaggggc tggctctgtc tgcaatcccg gtgattttta
     1561 cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat ggcaacattc
     1621 gtaagctacg ctgggtgttt ataatcggta gtgcgatccc cctggtggca tatattttct
     1681 ggcaggtggc gacgcttggc agcattgatt caacaacctt tatgggattg ctggctaatc
     1741 atgctggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg
     1801 agctggcagt gcatttattt gctgatttag ccctcgccac gtcatttctc ggcgttgcgt
     1861 taggcttatt tgattatctg gctgatttat ttcagcgttc aaataccgtt ggtggacggt
     1921 tgcaaactgg tgcaattacg tttctgccgc cgttggcgtt tgcactgttt tatccacgag
     1981 gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca ttgattatcc
     2041 cttcgctgtt gacctggcaa agcagaaagc acaatcctca ggcgggttac cgggtcaaag
     2101 gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat
     2161 ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc aaattcctta
     2221 ttgggtgcca gaattaacgc tgacacccaa tttggcctct taatgcaggc agcactgctt
     2281 aaatttctta ccactaccgc acgggcaagg atcgttacgc cccggtttct cttctgcttt
     2341 gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtacgcat gtagatcaag
     2401 cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca tcttttctac
     2461 ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg gttttaacga
     2521 atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa agcaccactc
     2581 ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg gctcaaactg
     2641 ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag ccagattcat
     2701 aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc caccccacac
     2761 ggcaaccagc cactgttccg gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa
     2821 accgtccagc tccgccacat caaggatggc gtggtcagtg ttgtatttgg tcagaatatc
     2881 gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg
     2941 gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata ctttttgagt
     3001 ggctttgctg gtgattaaaa attagggagg gtgtaacgac aagttgcagg cacaaaaaaa
     3061 ccacccgaag gtggtttcac gacactgctt attgctttga ttttattctt atctttccca
     3121 tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt ttaaatccct
     3181 tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg
     3241 aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc gccaaattct
     3301 tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg agcgggaaac
     3361 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
     3421 cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca atcagtgccg
     3481 ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat ttttcaccac
     3541 ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa tctgcacgcg
     3601 cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca aagaaaagtg
     3661 caataccggc gtactcaacc caaatgttcg gacgccacag cagccatgcc aacgccacca
     3721 tctgggcagt ggttttcact ttcccaatcc aggagacggc cacgctactg cgtttaccca
     3781 actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc atcgttgccg
     3841 ccggtaatgt cacccaccag ctgtgataat gctcggttac cagcaccatg gcgatagcca
     3901 cgagaacttt atctgccaca gggtcaagga aagcaccaaa ccgggtactc tggttccagc
     3961 ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg
     4021 cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat gggataagga
     4081 tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg ggtaactatc
     4141 tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca acgaccagaa
     4201 gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct cgacgctggc
     4261 gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac gttttggccc
     4321 gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccttt ttttacggtg
     4381 cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc tggataacat gcagcgcggg
     4441 tgaatctggc ggcaaactaa atccctcacc ttccggctca aagaacagtg tttccagccc
     4501 agccttacga tctgctcctt tggcaacgcc aagtagcagt ggatgatttt tatcccatga
     4561 gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat
     4621 aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc gacgcagcac
     4681 ctgattcatc gccgcataat catcgcccgg cgtgatacct gtaatgttat agcgccgata
     4741 ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg tttgttcgcc
     4801 catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt tcaacacgct
     4861 ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc tggttaaggc
     4921 cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt
     4981 ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca gcgttttatc
     5041 gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa
     5101 atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc caggcacttt
     5161 cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata cgtggacaca
     5221 agccatgccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg tattggaaac
     5281 gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac gtgcagcttc
     5341 ttcaaactcc agattctggc tggcagtttc catacgacta atgagttgcg taagcacctg
     5401 atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt attcttcttc
     5461 actcaccagt ccttcaacgc acggtcccag acagcgccct atctggtatt gcagacacgg
     5521 acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa tcttttgcag
     5581 tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat attcaccttt
     5641 ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg gtatcaccac tcaggaagat
     5701 aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat
     5761 gtagttgtgt tccagcaaca gcgcttcggt ttctgtgtga gtaaccgtta catcaatttg
     5821 ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctac ggaaatagct
     5881 ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac caccagcatc
     5941 gtacatgcga taaacgcctg gctggctggt tacggttttt aaaaacgctt ttgcgtcaaa
     6001 ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg aattgccagg
     6061 tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata gcggtagctg
     6121 ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt ctggcccttg
     6181 gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg gctttctgtt
     6241 ttttctggtt cgatctggct taacgccatt tgttgagcga tgtcagaagc aatgtaacgc
     6301 tgccctgaat agacagaacg aatcgcactc acaacttcct gcggagccgc gcctttgctg
     6361 aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc tgtatggacg
     6421 gtaagcatga tgattttgac atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga
     6481 ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt tgtccggcac
     6541 cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc ctttatatct
     6601 tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag tagaacgttg
     6661 atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca ttcgtatatt
     6721 agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt
     6781 ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc ttaccggatc
     6841 gtttcacagt aagccctacg atgaaatgtg acaaaaattg actttattca gcaaaaatga
     6901 aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca aaaaacat
//
LOCUS       DAPHDX010000188         6908 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPHDX010000188 DAPHDX010000000
VERSION     DAPHDX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.191"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1962
                     /gene="acnA"
                     /locus_tag="RH058_004112"
     CDS             <1..1962
                     /gene="acnA"
                     /locus_tag="RH058_004112"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV3139616.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2026..2616)
                     /gene="ribA"
                     /locus_tag="RH058_004113"
     CDS             complement(2026..2616)
                     /gene="ribA"
                     /locus_tag="RH058_004113"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV3139617.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2786..3550
                     /gene="pgpB"
                     /locus_tag="RH058_004114"
     CDS             2786..3550
                     /gene="pgpB"
                     /locus_tag="RH058_004114"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV3139618.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3699..4007
                     /gene="lapA"
                     /locus_tag="RH058_004115"
     CDS             3699..4007
                     /gene="lapA"
                     /locus_tag="RH058_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV3139619.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4014..5183
                     /gene="lapB"
                     /locus_tag="RH058_004116"
     CDS             4014..5183
                     /gene="lapB"
                     /locus_tag="RH058_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV3139620.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5376..6113
                     /gene="pyrF"
                     /locus_tag="RH058_004117"
     CDS             5376..6113
                     /gene="pyrF"
                     /locus_tag="RH058_004117"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV3139621.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6113..6439
                     /gene="yciH"
                     /locus_tag="RH058_004118"
     CDS             6113..6439
                     /gene="yciH"
                     /locus_tag="RH058_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV3139622.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6565..6783)
                     /gene="osmB"
                     /locus_tag="RH058_004119"
     CDS             complement(6565..6783)
                     /gene="osmB"
                     /locus_tag="RH058_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV3139623.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         1692 a         1629 c         1852 g         1735 t
ORIGIN      
        1 ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac cggtaaatta
       61 cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat
      121 ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact accgctggcg
      181 gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca
      241 atcgatgctg taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggttgag
      301 ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt
      361 tttaccagta cgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa
      421 cgcccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa
      481 ctggaagtga atgccacgca taaagatcgc cagccggtcg attatgttat gaacggacat
      541 cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc
      601 tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactctgggc
      661 ctcaagcggc aaccgtgggt caaagcgtcg ctggcaccgg gttcgaaagt cgtttctgat
      721 tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa ccttgtggga
      781 tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca
      841 atcaaaaaag gcgatttaac cgtcggtgca gtgctgtccg gcaaccgtaa ctttgaaggc
      901 cgtatccatc cgctggttaa aactaactgg ctggcctcgc cgccgctggt ggttgcctat
      961 gcgctggcgg gaaatatgaa tatcaacctg gctgctgagc ctattggtca tgatcgtaaa
     1021 ggcgacccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta
     1081 gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca
     1141 gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc
     1201 tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat
     1261 attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct
     1321 ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt
     1381 gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc
     1441 ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg
     1501 acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag
     1561 caggaacaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt
     1621 gactgggcgg caaaaggtcc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt
     1681 gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa
     1741 ggcgtaacgc gtaaaacgtt agaactaacc ggggaagaga agattgatat tggcgatctg
     1801 caaaatctac aacccggtgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag
     1861 gaggtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac
     1921 gacggcattt tgcattatgt cattcgtaat atgttgaagt aacaagtatt tgcttgccgg
     1981 ttattttgct tccggcaagc aaactaatta cacaatgcaa gagggttatt tgttcagcaa
     2041 atggcccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac
     2101 aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt
     2161 cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca
     2221 aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc
     2281 gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc
     2341 ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc
     2401 ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc
     2461 ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcgac
     2521 atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt
     2581 tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt
     2641 ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg
     2701 ttcacggaat ggcacaaatc tgtcaatttt tcctggaact ggcgttttca gttatgattg
     2761 tgggacttat caaaaaggag aggccatgcg ttcgattgcc agacgtaccg cagtgggagc
     2821 tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg
     2881 agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg
     2941 gggcgtcatt acacatttga ttttattcgg ctggtttctc tggtgtctgc gttttcgcat
     3001 taaggctgcc tttgtattat ttgccattct ggcggccgca atccttgtgg gacaaggcgt
     3061 taaatcctgg atcaaagaca aagtccagga accacgacct tttgttatct ggctggaaaa
     3121 aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac gcggaaattt
     3181 agtgaaagaa cagttggctg aagagaaaaa tatcccacaa tatttgcgtt cacactggca
     3241 gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact
     3301 gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgctggt
     3361 ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct
     3421 ggtagtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg
     3481 aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaatagcgc aacgagaaca
     3541 agaaagttaa cgctggttga ttttccgaat ttagccctta aatcatcaac aatgcgcgtg
     3601 gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac
     3661 cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct
     3721 tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg
     3781 tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat
     3841 tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc
     3901 gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg
     3961 cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg
     4021 agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa
     4081 gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg
     4141 ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta
     4201 aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg
     4261 gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct
     4321 atgaacagcg tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat
     4381 acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg
     4441 cgctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg
     4501 ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct
     4561 actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc
     4621 taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg
     4681 tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtgatatccc
     4741 aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg
     4801 gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg
     4861 atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gccgcacagg
     4921 tctatattac gcgccagctt cagcgtcatc cgaccatgcg tgtgttccat aagttaatgg
     4981 attaccactt aaatgaagcg gaagaagggc gtgccaaaga gagcctgatg gtgctgcgtg
     5041 acatggttgg cgaaaaggta cgtagtaagc ctcgttatcg ctgccagaaa tgtggtttta
     5101 ccgcatacac cctctactgg cattgtccgt cttgtcgggc ctggtcaacc attaaaccga
     5161 ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag
     5221 ttattgatgt tccattgtgc tccggcaacg acggcgcaga aaaagcctgc caggggaaaa
     5281 atcgcaactg ttaatttttt atttccacgg gtagaatgct cgccgtttac ctgtttcgcg
     5341 ccacttccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt
     5401 cccgcgctgt tacgaattct cctgtggttg ttgcccttga ttatcataat cgtgatgacg
     5461 cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggcaaagaga
     5521 tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct
     5581 ttcttgacct gaaattccac gatattccta acactgctgc gcacgctgtc gctgctgcag
     5641 ctgacttagg cgtgtggatg gtgaatgttc atgcctctgg tggggcgcgt atgatgaccg
     5701 cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag
     5761 tgttgaccag catggaagcc agcgacctgg tcgatcttgg catgacactg tcacctgcag
     5821 attatgcaga acgtctggcg gcactgacgc aaaaatgtgg ccttgatggt gtggtgtgtt
     5881 ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacgc
     5941 cgggcattcg tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac
     6001 aggcgttgtc ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag
     6061 atccagcgca gacgctgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga
     6121 ttccaacagc cgtctggtct actcaacgga aaccggacgt attgatgagc ccaaagcggc
     6181 ccctgtacgc cctaaaggcg acggtgtggt gcgtattcag cgtcagacca gtggacgtaa
     6241 aggtaagggc gtttgcctga ttaccggtgt cgatctcgat gatgccgaac tgacaaaact
     6301 tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga
     6361 aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt
     6421 aaaactcgca ggtggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt
     6481 actgtgtgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
     6541 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
     6601 agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc
     6661 ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact
     6721 cagagacatt gccagggtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
     6781 cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc
     6841 taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg
     6901 caggtgat
//
LOCUS       DAPHDX010000189         6885 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPHDX010000189 DAPHDX010000000
VERSION     DAPHDX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.192"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            430..912
                     /locus_tag="RH058_004120"
     CDS             430..912
                     /locus_tag="RH058_004120"
                     /inference="COORDINATES: protein motif:HMM:NF024954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3139624.1"
                     /translation="MGQQRGRLSPFLERCKIISLVHEAVINGARKSQACRCIGISIRT
                     LQRWTNGNVITTDRRSDTVNRSPRNKLSEAERLRILELCNSPEFASLPPNVIVPTLAD
                     RGIYIASESSFYRVLKSANQLKKRSRECQYKRPDVLRAVAPNQVWSWDISYLPSWVRG
                     "
     gene            1122..1277
                     /locus_tag="RH058_004121"
     CDS             1122..1277
                     /locus_tag="RH058_004121"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3139625.1"
                     /translation="MLSSYSRPRVSNDNPYSESLFRTLKYCPWWPENGFYTIDDARVW
                     VNRFVNW"
     gene            1573..1918
                     /locus_tag="RH058_004122"
                     /pseudo
     CDS             1573..1918
                     /locus_tag="RH058_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2114..2974)
                     /locus_tag="RH058_004123"
     CDS             complement(2114..2974)
                     /locus_tag="RH058_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV3139626.1"
                     /translation="MTTATSLNELQKQIRDRYDSLSKRLQQVSRYVLDNTNSVAFDTV
                     AVIAERADVPPSTLIRFANAFDFTGFNEMKQLFRMHLVEETASYSDRARLFREMESKA
                     VPETPLDILQEFASSNSQALQQLAARAEPEMLEHAVQLLAYADTIYIAGLRRSFSIAA
                     YLTYALSHLECRPILLDGMGGMLREQISRIKACDIVVSISFSPYAEETVMVSKTAASV
                     GARQIVITDSQISPLATFSDLCFVVKEAQVDAFRSQSATLCLVQSLVVALAYRLGDKK
                     HNNTQENSNQ"
     gene            3265..4770
                     /locus_tag="RH058_004124"
     CDS             3265..4770
                     /locus_tag="RH058_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3139627.1"
                     /translation="METVANFIHGECVTGSGQRIQAIFNPATGEQIRQVVMSTAQETE
                     QAIAAAQLAFPAWARFAPLKRARVMFRFKALLEENMERLARIISEEHGKVFSDAVGEL
                     TRGLEVVEFACGIPHLQKGEHSANVGTGVDSHSLMQPLGVCAGITPFNFPAMVPMWMF
                     PIALATGNTFVLKPSEKDPSLALALAKLLKEAGLPDGVFNVVQGDKESVDVLLTDPRV
                     QAVSFVGSTPIAEYMYLTASAHGKRCQALGGAKNHCILMPDADMDMATNAIMGAAYGA
                     AGERCMALSVVVAVGDKTADELCARLEKKIASLRVGPGLNQTPENEMGPLISAAHRSK
                     VLGYIDSGEAQGANLRVDGRGLRVKGHEQGYFVGPTLFDNVTPEMTIYKEEIFGPVLS
                     VVRVADYASAVDLINRHEYGNGSAIFTRDGGCARRFCEEVQAGMVGVNVPIPVPMAFH
                     CFGGWKRSIFGALNVHGNDGVRFYTRMKTITARWPEMQQETGAAFSMPTLG"
     gene            4782..6707
                     /gene="iolD"
                     /locus_tag="RH058_004125"
     CDS             4782..6707
                     /gene="iolD"
                     /locus_tag="RH058_004125"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDV3139628.1"
                     /translation="MQTERMTMAQALVRFLNQQYVSVDGKETPFVEGVATIFGHGNVL
                     GIGQALEQDPGHLQVMQGCNEQGIAHMATGFAKQHRRQRIFAVTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDIYACRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPE
                     QLMSAMLSAMRTLTDPANTGAVTICLPQDVQGEAWDYPLSFFARRVHHIERRPPDPQR
                     LAQAHALISRKRRPLVVCGGGVRYSGAHEALREFVEVLQLPFAETQAGKGALVSDHPL
                     NLGGVGVTGGMAANQLAPQADLVIGIGTRLTDFTTGSKALFSHPDVEFLLLNVAEFDS
                     LKLDATALIADARTGLTALTHALKDYRSGWGEAIAHAKSAWHDECCRLWERQWHPDDV
                     PEVAGHLDAQLMEYGETLNTHLTQTRVLGLINQHIEDNAIVVGAAGSLPGDLQRLWQV
                     KTPDSYHLEYGYSCMGYEVAAAIGAKLAKPQQPVYAMVGDGSYMMLHSELQTAVQEGI
                     KVTILLFDNASFGCINNLQMGHGMGSFGTENRYRNPKTGQLDGPLVKVDFAQNAESYG
                     CRAWRVHNEKSLLAALEASRAHPGPTLLDIKVLPKTMTHDYASWWRTGNAQVAESPAV
                     RQAAEQTQAQVKKARQY"
BASE COUNT         1676 a         1705 c         1866 g         1638 t
ORIGIN      
        1 tattaatcgt attatttagc acacataata gatattaact ttttgttcgt aagcgttaac
       61 ggaacactgt attgacgttt atttattggt gagaactacg ttccttggca ggagttcgtc
      121 aacacagttg gaagtctatt ccggcagtaa gctcaggata tgaagcagat acgcttctgg
      181 aacgattccg ttcagttggc aggtaccgat tcgtccatgc gcagtgcacc gtgctttccg
      241 ctgtaatagc tgccaaagga cacggttatc aaataaatct ggatattgat ggaaaatatt
      301 cgaacagaca gggcattgcg tgacgcaaaa aaataagtta cgagaactgg agaaagagct
      361 taagcgcaaa gataaagctc tggcggaagc tgcggctctg cttatgttaa gggaaaagtt
      421 caatgccctc tgggacaaca gcgaggaaga ctgtcccctt tcctggagcg gtgtaaaatt
      481 atttctcttg tccacgaggc cgttatcaac ggcgcccgga aaagtcaggc ttgtaggtgt
      541 atcggtattt ctattcggac acttcagcgc tggactaatg gcaacgtcat caccacggac
      601 cgaagatccg atactgtaaa cagatctccc cgaaataagc tgtcagaagc ggaaagattg
      661 cggatactgg agttgtgcaa ctctccagag tttgcatcct tacctccaaa tgtgatcgta
      721 ccaactctgg cagacagggg tatttatatc gcttctgaat caagctttta tcgtgtactg
      781 aaatcagcca accagttgaa gaaacgctcc cgtgaatgtc agtataaacg ccctgatgta
      841 ctcagggccg ttgcgccgaa tcaagtctgg agttgggaca tcagctattt gccgtcctgg
      901 gtcagaggct agcattttta cctctatatg atcgtcgata tttacagtcg caagattatt
      961 gctgcagagg tatttgccag cgaatccgga aaacatgcag cagaactact ccagcgggca
     1021 gtatggaatg aaaaatgttc atccaacaat ctggtactcc attctgataa tggtggtccc
     1081 atgcgcagtt gcacgttgtt ggcgaaaatg tatgcgctgg tgtgctaagt tcttattccc
     1141 ggccaagagt cagtaatgat aatccttact cagaatcttt gttccggact ctcaaatatt
     1201 gtccctggtg gcctgagaac ggtttctata ctattgatga tgccagagta tgggtaaacc
     1261 gttttgtaaa ctggtaaaac tttgaacata agcacagcgg gatcaaatac gtcacgccag
     1321 ccgagcgtca tcaaggtaat gacatgaaaa tactgatagc cagaaaagca gtatatcaac
     1381 tggcacgcga gcaacatcct gaacgttgga gcaagcatct acgtaaattg gactacatat
     1441 cagaagtcta cctgaatcca gaaaaagagg ctgcataacg taattttatt acaaaagtcg
     1501 acaactacct tgaaaaacgc cgccaatccg tgatcgaata gagtggaata cgcaccacta
     1561 tcatttaaat aagtgaaagt cttgctacac gtgttcagac tagtcgttgg agaacagatg
     1621 attgatgtct caggatcgga gaaacgcaga ctgtgcatca cacacgcaaa atcgctactg
     1681 ttcaacagag ttttgaacca ggaatgatgg tcttcctgat tgcctggcaa catggtatgg
     1741 tggtcagccc gttatttctc tggcgtatca ttaccttgaa ggaagcttca ctactgtgac
     1801 agccgaagaa cagggtattc cggcctctga acttgctgcc gccatgaata aaattaaaga
     1861 acttcagcgc ctgctaggta agaaaacgat ggtaaataaa ctgtttaaag aagccgttat
     1921 atctgggtta cattaccacc tactagagaa atcttcttcg ctattaagga ttaaggctct
     1981 cgttgtacgc tcacatgggg catgaccttt tagggagata gcagtacagt tgatattcaa
     2041 tctgaagtct gtatcgctac tggcaccgaa tcatttactc ttatatggac tcgggggagg
     2101 agttcattgg ttattattgg ttactgtttt cctgggtatt attatgtttt ttatctccta
     2161 aacggtaagc tagcgcgacc actaacgact gcaccagaca caatgttgcc gactgagacc
     2221 gaaatgcgtc cacctgagcc tctttcacca caaagcagag atcactgaac gttgccagtg
     2281 ggctgatctg gctatccgta atcacaatct gccgagcgcc gacgctggcg gcggttttgc
     2341 tgaccatcac cgtttcttcc gcgtacggcg agaaactgat cgacaccaca atgtcgcacg
     2401 ccttaatacg gctgatctgt tcgcgcagca tgccgcccat cccgtcaagc agaatagggc
     2461 ggcactctag gtggcttaaa gcataggtaa gataagcagc aatgctaaag gatctgcgta
     2521 atccggcgat gtaaatggtg tcggcgtagg ccagtagttg tacggcgtgc tccagcatct
     2581 ccggttcggc gcgggcagcc aactgctgca gcgcctgcga gttcgaactg gcgaactcct
     2641 gcaaaatatc gagcggcgtt tcaggtacgg ctttgctctc catttcgcgg aacagtcgcg
     2701 cacgatcgct gtagctggcg gtttcctcca ccaggtgcat acgaaataac tgtttcattt
     2761 cgttgaagcc ggtgaagtca aaggcattgg caaaacgaat cagtgtagag ggtggaacat
     2821 cggcacgttc ggctatgacc gcaaccgtat caaacgcgac actgttcgta ttatccagta
     2881 catagcggga gacctgctgc agccttttgc tcaggctatc gtagcgatcg cggatttgct
     2941 tttgtagttc gttcaggctg gttgctgtcg tcatgttatc cccctatcca gtctcatgat
     3001 tctaaacgca aaaccgcgtc ttttaaatgt tggccatcca cctggcagca aaaatgaaac
     3061 agaagcttca gtcatttcct cgcccgaaac tcctaatagc tcaggctgag tcagtgatgc
     3121 tggctgttca cataaaaatc catccttgat cacaataatc attatttatt ttatttcaac
     3181 ttaaaatgga atatttgttt catatatagt gttcagtaca taacgttcag gtaacaatca
     3241 cgtatcaaca gcgagaggtt aaggatggag acggtagcga attttattca cggcgaatgt
     3301 gtcaccggtt caggccagcg cattcaggcg atcttcaacc cggccactgg cgagcagatt
     3361 cgccaggtgg tgatgagtac ggcacaggaa accgaacagg cgattgccgc agcacagttg
     3421 gcgtttcccg cgtgggcacg ttttgctcca ctcaagcgcg cgcgtgtgat gttccgcttc
     3481 aaagcgttgc ttgaagagaa tatggagcgt ctggcccgta ttatcagtga agaacacggt
     3541 aaggtcttct cagatgcagt aggtgaactg acccgtggtc tggaagtggt agaattcgcc
     3601 tgcggcatcc cacatctgca aaaaggcgaa cactctgcga acgttggcac aggtgtcgac
     3661 agccactcac tgatgcagcc gctcggggtc tgcgccggga tcacgccgtt taacttcccg
     3721 gcgatggtgc caatgtggat gttcccgata gctctggcga caggcaacac cttcgtgctg
     3781 aaaccgtcgg aaaaagatcc gtctctggcg ctcgcgctgg cgaaactgct gaaagaggca
     3841 ggcctgccgg acggcgtatt caacgtggtg cagggcgata aagaatccgt tgatgtgctc
     3901 ctgaccgacc cgcgcgtgca ggccgtcagt tttgtcggtt caacaccgat tgccgaatac
     3961 atgtacctga ctgcgtccgc ccacggtaag cgctgtcagg cgctcggcgg tgcgaaaaac
     4021 cactgcattt taatgccgga tgccgatatg gatatggcga caaacgccat aatgggcgca
     4081 gcttatggtg cggcaggaga acgctgcatg gcgctttccg tggtggtggc ggttggcgat
     4141 aaaacggctg atgaactctg cgcccggctg gaaaaaaaga tcgcttcgct gcgcgtcgga
     4201 cctggcctga accagacgcc agaaaacgag atggggccgc tgatctccgc cgcacaccgc
     4261 agcaaagtgt tgggctatat cgacagtggc gaagctcagg gggctaacct gcgtgtggac
     4321 gggcgtggcc tcagagtgaa agggcacgag cagggctatt tcgttgggcc gactctgttc
     4381 gataacgtca ccccggagat gaccatctat aaagaagaaa ttttcggacc ggtgctttct
     4441 gtggtgcgcg tagcagatta cgccagcgcg gtggatctga ttaaccgaca cgaatatggt
     4501 aatggctcgg ccattttcac ccgtgacggt ggctgcgcac gccgcttttg tgaagaggtg
     4561 caggcgggta tggtgggggt caacgtgccg atcccggtgc caatggcatt ccactgtttc
     4621 ggtggctgga aacgttccat ttttggcgcg ctcaatgttc acgggaacga tggcgtgcgt
     4681 ttctacaccc gaatgaaaac catcactgca cgctggccgg aaatgcagca ggagacaggt
     4741 gcagcgttct ccatgccgac gctgggctga cagggggttt tatgcaaacc gaacgtatga
     4801 ctatggccca ggcgctggtc agatttctta accaacaata tgttagcgtt gatggtaaag
     4861 aaacgccctt tgtggagggc gtggcaacga tctttgggca cggtaacgtg ctcggtatcg
     4921 gccaggcgct ggagcaggat cctggtcatc tgcaggtgat gcaaggctgt aacgagcagg
     4981 ggattgccca tatggcgacg ggattcgcca aacagcatcg ccgtcagcgt atttttgctg
     5041 tcacctcatc cgttgggcct ggcgctgcca atatggtgac ggcggcagcg acggccacgg
     5101 cgaaccgcat tccactgctg ctactgccag gggatatcta cgcctgccgc cagcctgacc
     5161 ccgtgttgca gcagatcgag cagtatcacg atctcagcat cagtaccaac gattgcttcc
     5221 gcccggtttc ccgctactgg gaccgcatca accgtcctga acagcttatg agcgcgatgt
     5281 taagtgcgat gcgaacgctt actgaccccg caaatacagg agcggtgacc atttgcctac
     5341 ctcaggatgt gcagggggag gcctgggatt accccctgag tttctttgcg cgtcgggtgc
     5401 atcatattga acgccgtccg cctgacccgc agcgactggc gcaggctcat gcattgattt
     5461 cccgcaagcg ccgtccactg gtggtgtgcg gcggtggcgt gcgttactcc ggtgcgcatg
     5521 aggcgttacg ggaatttgtt gaagtgctgc aattgccgtt tgccgaaacc caggccggta
     5581 agggggcgct ggtaagtgac cacccgctta atctgggcgg ggtgggcgtg acgggcggaa
     5641 tggctgccaa ccagcttgcg ccgcaggctg acttagtgat tggcatcggc acgcgcctga
     5701 cggattttac taccggctct aaggcgctct tttctcatcc cgacgtcgag ttcctgctgc
     5761 tgaacgtggc tgagtttgac tccctgaagc tggatgccac cgcgctcatt gccgatgccc
     5821 gaactggtct gacagcgttg acccacgcac tgaaagatta tcgtagtggc tggggggagg
     5881 cgattgccca tgcgaaatcc gcttggcacg atgaatgctg tcgactgtgg gaacgccagt
     5941 ggcatcctga cgacgttccc gaagtggcag ggcaccttga tgcacagcta atggaatacg
     6001 gtgaaacgct caacacacac ctgacccaga cgcgggtgtt ggggctcatt aaccagcata
     6061 ttgaggataa cgccattgtg gtgggggcgg ccggatcgct gccaggggat ttgcaacgcc
     6121 tctggcaggt taaaacgcct gacagttacc atctggagta tggttactcc tgcatggggt
     6181 atgaggtcgc agcggctatt ggtgcgaagc tggcaaaacc tcagcagccg gtgtatgcca
     6241 tggtaggcga tggttcgtac atgatgctgc actcagagct gcaaactgcc gtacaggagg
     6301 gaattaaggt caccattctg ctgtttgata acgccagctt cggctgcatt aataacctgc
     6361 agatggggca cggaatgggg agttttggca cagaaaatcg ttaccgtaat ccgaaaacag
     6421 gtcagcttga cgggccgttg gtgaaggtcg attttgcgca aaacgcggaa agctacggtt
     6481 gccgcgcatg gcgggtacat aatgagaaaa gcctgctggc ggcgctggaa gcctcgcgcg
     6541 ctcacccggg accgacgctc ctcgacatca aagtgctacc aaaaaccatg acccatgatt
     6601 acgcgtcatg gtggcgaacc ggcaacgcac aggtggcaga gtcgccagca gtgcgccagg
     6661 ccgccgagca aacacaggct caggtgaaaa aagctcgtca gtattgagtt ttcctgtcgt
     6721 aacgaaattt tcatttcatt tttctttttt cccctcaccc atcccctctc ccaacgggag
     6781 aggggatggt acagttttta ttccctcccc cccttgtaat ggagggacat atcttcctcg
     6841 gcgttgtgag attgataaca aaatttatct aatacatctc aaaag
//
LOCUS       DAPHDX010000190         6871 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPHDX010000190 DAPHDX010000000
VERSION     DAPHDX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.193"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(247..732)
                     /gene="sixA"
                     /locus_tag="RH058_004126"
     CDS             complement(247..732)
                     /gene="sixA"
                     /locus_tag="RH058_004126"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV3139629.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMNPCNLKMAKA
                     I"
     gene            complement(935..3079)
                     /gene="fadJ"
                     /locus_tag="RH058_004127"
     CDS             complement(935..3079)
                     /gene="fadJ"
                     /locus_tag="RH058_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV3139630.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPSSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3079..4389)
                     /gene="fadI"
                     /locus_tag="RH058_004128"
     CDS             complement(3079..4389)
                     /gene="fadI"
                     /locus_tag="RH058_004128"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV3139631.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4569..4853)
                     /locus_tag="RH058_004129"
     CDS             complement(4569..4853)
                     /locus_tag="RH058_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV3139632.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5225..6565
                     /gene="fadL"
                     /locus_tag="RH058_004130"
     CDS             5225..6565
                     /gene="fadL"
                     /locus_tag="RH058_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV3139633.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1720 a         1842 c         1627 g         1682 t
ORIGIN      
        1 gattattcaa ctctattatc tgtattaata aatgacaatg agtgtcaaac agacattatt
       61 catttcaaca tgtaactttg ggcaaaatca agatacgcgc agaagaataa tttccgaatg
      121 aatgtagaaa gtgttttatt tatagataag gagagtcttc gatagtagtg aggttgacag
      181 atatccgctg cctgatgagc aattatgccc aatgaatcta cctcattggg cataatttgg
      241 aacacatcag atagcttttg ccatcttcag attacacggg ttcatctgcc agttgaatgt
      301 accgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa acataggcgg
      361 cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt gagagatcac
      421 cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga ccagaccaac
      481 atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt tcagacaatc
      541 ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa cgcgttcgat
      601 ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac aaccattagt
      661 ggtcaaagga cgaaccgaat cactggcggc atcgagggct gcgtcgccgt gacgcatgat
      721 aaaaacttgc atattgcacc gcttttgtta accagattcg cccgttttct ttcatctgaa
      781 accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa tgaaacgctg
      841 ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac caacactccg
      901 ccattcagcg cggattcata tagctttgac cttcttattg caggtcagtt gcagttgttt
      961 tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg gtaaaacggg
     1021 aaccatactg cgtggcaagt cgttgcatta ttgcaaccac ttcgcccgcg ccgagagaat
     1081 cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat accgcgccaa
     1141 tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact gcttcattca
     1201 gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc tgcccttgtg
     1261 cgccaatgag cgggtaaatg gcaggatcga cctgtttttt gcttttacgc cctttctgac
     1321 cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc aaaattgaag
     1381 aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt acaggaataa
     1441 ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattggacct accggaaaac
     1501 caaatttcac tagcgcagca tcaatgtgct ctacccgttc accttcggtc agcatgcgga
     1561 tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct ttgtcacgca
     1621 cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg atggtttgcg
     1681 ctgatgtacc cgcatgagga ataatctcca ccagcggcat tttttccacc ggactgaaga
     1741 aatgcaggcc gataacttgc tcaggtcgcg tggcgtgagc ggcgatatca ccaatcggta
     1801 aagatgacgt attcgaagca aagatggtat gagcggcgct attttgctca acttccgcca
     1861 ccatctgttg tttcaattcg agattttcaa acaccgcttc aataatcaga tcgcgatggg
     1921 caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca cgttcgctgg
     1981 ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac ttcagcgcat
     2041 gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct ttacaagcag
     2101 tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg ctgtttaatg
     2161 gcgcaggcgg tgcatcacta ctgggatctt tcttcacgtc cgtactggca aaaaagatac
     2221 tacgcagcgc ctgcgattgt ggcgtcatcg ccagttcgcc aaacgcccga gcttcggcgt
     2281 cataaccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg atgcgttctg
     2341 tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt ttgaacagca
     2401 gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg cgggaagatg
     2461 ggcgatcctt cttcgccagc tcaacagcga cttccagcaa aatggagtgc ggaacaacgt
     2521 catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg gtgaggatca
     2581 tctctaatgc tgtgctgacg cctatcagac gcggtaaacg ctgggtgccg cctgaaccgg
     2641 gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg tcagtacaaa
     2701 cgcgaccgtg gcacgccagc gccaactcca gcccaccacc caggcaagcg ccatgaatag
     2761 ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg ccctgccgcg
     2821 ccagtgcttc cgcttcttgc gccgttttgc agttgccgat catgttgatg tctgcgccag
     2881 caatgaagtt gtccggttta gcggagacaa acaccacgcc tcgcaactct ttgttttcac
     2941 ggagttgctt aataatggcg cgcacctgcg aggcaaactc cgccttcagg gtattcattt
     3001 tctcacccgg tacgtcgatg gtgatgatag caatgttgtc cagacgaaca ttaagagtaa
     3061 acactgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa gcccaccggc
     3121 agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat gcaatgtctg
     3181 ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgcccg taagcaatcg aaccgccaag
     3241 cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca gtacttcacg
     3301 agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg caaaggcttc
     3361 gtgcatatcg atcaatgtca gatcgctcat tgtcaaaccg gcacgttcca gcgccagcgg
     3421 tgttgaccag gccggaccga gcaacatgtc ctgccagaca tcaatcgcag taaatgcgta
     3481 gctgcgcaga taccccagcg gtaccagccc taattctttc gcccgggatt cagtcatcag
     3541 gatcaccgcc gctgcgccat cggtcagcgg cgtactgttc gccgccgtta ccgttccgtg
     3601 tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat taccgcgaat
     3661 attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca tcacctcttc
     3721 tttgagttta ccgtctgacc acgcctgagc ggcacgctga tgcgaacggt gcgctaatgc
     3781 atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg tgtcgcccat
     3841 ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt cgcgcaaacg
     3901 caggcgagag aagagtttca gtcgctggct catggttcga gctttgttga catcaaccag
     3961 cacgcgcgcc agttttttac tgacgccaat tggcaatacg gaagaggaat ctgccccacc
     4021 ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg caaccgcctg
     4081 gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat tcattccggt
     4141 accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga cctgaccaaa
     4201 gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt cgcctaccac
     4261 catcttccct aaatcaaccg cgggaatgcc atgaaaagcc gtcgcctggc gggcaaaagg
     4321 cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca gcggtaaaac
     4381 ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg atcatagtct
     4441 taaccatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct atgacacagt
     4501 gaagacagga gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc gaacgtaccc
     4561 gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca ggcaaaagtg
     4621 aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga gattttgcac
     4681 ggttcggatt ccacgctacg ggctttttca gtcagcgccg ccagcgtttg ttctgcttct
     4741 gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc cataatggtg
     4801 ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact catcgttgat
     4861 ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta cgcttccgga
     4921 aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat cacacttaaa
     4981 aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa ttttgccagc
     5041 tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc aacattccag
     5101 ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc agaccctaca
     5161 cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca ttgaggttat
     5221 ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag tggcacttat
     5281 ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct ctggcctggg
     5341 ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta gccgtaaccc
     5401 cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt atattgaccc
     5461 ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg ataacatcgc
     5521 gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc aatttggttg
     5581 gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata cttatgcagg
     5641 cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa gcggtgcgta
     5701 tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccgtctacg ctcgcgcgaa
     5761 aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta tgcaatctcc
     5821 tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta tcgacagtaa
     5881 taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg ccggaatcct
     5941 gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag tgaaaattga
     6001 cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg gtttgccaat
     6061 tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc tgcctgaaat
     6121 gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact atagcctggc
     6181 ttacaccagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg gcgacacgct
     6241 gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta ccacttatta
     6301 ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc cagttccggc
     6361 tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg caggtacgac
     6421 ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc acggtcagag
     6481 cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct ggctgttcgg
     6541 tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca cctgcatagc
     6601 aggtgacttt aactccccca cttcaccgaa agtagtgtcc tcattgctta cctcctgagt
     6661 tttgcaaaca gcctgttggc agcttgccct ttcaaatcaa taagcggtat ccacagacaa
     6721 acctgaaggg aaaggcatat tttcaggcgt tctgctcgtc cttctcaaag agtttacttt
     6781 tctgcatttc caggatactc aacccccctg gctattgtgc gctcatacac tcaaattaaa
     6841 gataggttct aaataaatga gcgttttttg a
//
LOCUS       DAPHDX010000191         6707 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPHDX010000191 DAPHDX010000000
VERSION     DAPHDX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.194"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..855
                     /gene="exuR"
                     /locus_tag="RH058_004131"
     CDS             79..855
                     /gene="exuR"
                     /locus_tag="RH058_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV3139634.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1200..1862
                     /locus_tag="RH058_004132"
     CDS             1200..1862
                     /locus_tag="RH058_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3139635.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1866..2249
                     /gene="mzrA"
                     /locus_tag="RH058_004133"
     CDS             1866..2249
                     /gene="mzrA"
                     /locus_tag="RH058_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV3139636.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2396..2764
                     /locus_tag="RH058_004134"
     CDS             2396..2764
                     /locus_tag="RH058_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV3139637.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2802..3107
                     /locus_tag="RH058_004135"
     CDS             2802..3107
                     /locus_tag="RH058_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV3139638.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3110..3514
                     /locus_tag="RH058_004136"
     CDS             3110..3514
                     /locus_tag="RH058_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV3139639.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3504..3803
                     /locus_tag="RH058_004137"
     CDS             3504..3803
                     /locus_tag="RH058_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV3139640.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3989..4381
                     /locus_tag="RH058_004138"
     CDS             3989..4381
                     /locus_tag="RH058_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV3139641.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4451..5437
                     /gene="yqjG"
                     /locus_tag="RH058_004139"
     CDS             4451..5437
                     /gene="yqjG"
                     /locus_tag="RH058_004139"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV3139642.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5730..6095
                     /locus_tag="RH058_004140"
     CDS             5730..6095
                     /locus_tag="RH058_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3139643.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6337..6693
                     /locus_tag="RH058_004141"
     CDS             6337..6693
                     /locus_tag="RH058_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3139644.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
BASE COUNT         1692 a         1617 c         1654 g         1744 t
ORIGIN      
        1 atttatatat gaccaatggc atacccatga ataaagtggt atgataactt cgtccgcatt
       61 ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca acaacttgcc
      121 gctgacctga aagagcgcat cgaacagggc gtctatctgg taggtgataa actgcctgca
      181 gaacgcttta ttgccgatga aaagaacgtc agccgtacgg tagttcgtga agccatcatc
      241 atgctggaag ttgaaggcta tgtcgaagtg cgtaaaggtt caggtattca tgtggtttcc
      301 aaccagccgc gccatcagca ggcagctgac aataatatgg aattcgccaa ttacggtccg
      361 tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt cgcggcaact
      421 caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc gcgcggcgaa
      481 caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc cctggcgacg
      541 cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccaacgtag ccataacccg
      601 tactggaaaa aactgcacga acacattgat tcccgtaccg tcgataactg gtgtgatgac
      661 cacgatcaaa tcctcaaggc gctgattcgc aaagatcctc atgctgctaa gctggcaatg
      721 tggcagcacc tggaaaacac caagatcatg ttattcaacg aaaccagcga cgacttcgag
      781 ttcaatgccg accgctatct attcgccgaa aacccggtgg tgcatctcga tacggccacc
      841 agtggcagta aatgaaatac ctgtccgaca ggcgctgcga cgcgcctgtt tgtctcgtcg
      901 gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct cccctgcatt
      961 cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg acctttgtta
     1021 caattagatt caatttgaat ttatgttttt gaatgctttc ttatctcacg atttaacagg
     1081 gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac agcaataaca
     1141 ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag gaataatgaa
     1201 tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa accctggcca
     1261 atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc cttgaaaacg
     1321 gcttgcttcc agcggccttt ttaccgggcg acagtttact ggtattggtc ggcgtgttga
     1381 ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt gccgccagcc
     1441 tcggctgctg ggtcagctat attcaggggc gatggctggg caatacccgc accgtacaaa
     1501 actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt tttcataaac
     1561 acggtttatc ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca ctgctgccga
     1621 cgattgccgg gttatcaggg ctgaataacg cgcgctttca gtttttcaac tggatgagcg
     1681 gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa acgccggtat
     1741 ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg
     1801 tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt
     1861 aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc actgttttac
     1921 ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag tctacgctgg
     1981 cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc tggcatcacc
     2041 ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact ctgttaatta
     2101 catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga acattgcccc
     2161 atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg ctgacccggt
     2221 tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata tcactcactt
     2281 tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact accgcttcgt
     2341 tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt caagaatgaa
     2401 ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt atgccactac
     2461 cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg aaaaacacca
     2521 aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg ccaactgttc
     2581 agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag atgaggtggc
     2641 ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata agattgccaa
     2701 acgcgaacgg aaactggcag aagcgcagga agagctgaaa aagctggaag cgcgcgacta
     2761 ctaactcaca atagtcacta cttactcacc tggagaaaac tatgtcgaaa gaacacacta
     2821 cggaacatct gcgtgctgag ttgaaatccc tttccgatac gctggaagag gtgcttagct
     2881 catctggcga gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg gagcaggcac
     2941 tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa caaacccgtg
     3001 tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc gtgggaattg
     3061 gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac
     3121 tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat
     3181 catggttgaa atggtggaaa cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa
     3241 agctaatctc tttcaacttt tactgatgct gggcctgacg atgcttttcg ctgcatttgg
     3301 tcttatgagc ctgatggtgc taattatttg ggcggttgat ccgcaatatc gcctgaatgc
     3361 gatgattgcc accaccgtgg tgttgctgct actggcactg attggcggta tctggacgtt
     3421 acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc catgagctgg caaacgatcg
     3481 gcaactgctc gaggaggagt cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc
     3541 aactgcttag ccagatccag caacaacggc tggatctttc cgccagtcgt cgtgaatggc
     3601 tggaggcaac aggcgcttac gatcgtcgct ggaatatgct gctaagtctg cgctcctggg
     3661 cgctggttgg cagtagcgtg atggcgatct ggacgattcg ccatcctaat atgctggtcc
     3721 gctgggccag acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa acgaccctca
     3781 agcagcaaca gcttcgcggt taacttccct ctggccggag caattccggc cttatccctc
     3841 aaattttttg aagatttttg acagttttcc ttgctaacaa tcatcattca ccacgtttat
     3901 gattctctcc atcgacagca acgacgctaa taccgcgccc ttgcacaaaa aaacaatcag
     3961 cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta gaagatgttg gtgtactggt
     4021 agcgcgcatt ttaatgccga ttctgtttat taccgcaggc tggggaaaaa tcaccgctta
     4081 ctctggtacc caacaatata tggaagcaat gggtgtcccg ggttttatgc tgccgctggt
     4141 gattctgctt gagtttggtg gtggtctggc aatcctgttc ggtttcctga ctcgcaccac
     4201 agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca gcaactttgc
     4261 tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca atttctggcg gattcctgct
     4321 gctggcaatt accggtccgg gcgcgtatag catcgaccgc ctgctgaata aaaagtggta
     4381 agcacgcgct atacttaacg ataaaaaacg aggaggaagc tcctcgtttt tgctattgga
     4441 ggagagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt acgataccaa
     4501 atctaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc tcactgccga
     4561 tggcgcacct ggccccactg gcacaggcgg ttttatcgca gagaaagatc gttatcatct
     4621 ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca aactcaaagg
     4681 actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa acggctggac
     4741 ctttgatgac agttttccgg gagcaaccgg agatacgctc tatcaacatg aatttctgta
     4801 tcagctttat ctccacgccg atccacacta cagcggacga gttactgttc ccgtgctgtg
     4861 ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca gaaatcatcc gcatgttcaa
     4921 taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac tacccaccag ccctgcaaac
     4981 gaaaattgac gaacttaacg gctggattta tgacaccgtt aacaacggcg tgtataaagc
     5041 tggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct
     5101 ggcgcgactg gagcagattt taggccagca tcgttacctg accggcaacc agctaaccga
     5161 agccgatatt cgcctgtgga ctacgctggt gcgttttgat ccagtgtatg tgacccactt
     5221 caagtgtgat aagcaccgca tcagcgatta cctgaatctg tatggcttcc tacgcgatat
     5281 ctaccagatg ccggggatcg ccgaaactgt caatttcgat cacatccgta atcattactt
     5341 ccgcagccat aagaccatca accctacggg gattatttca attggtccat ggcaggatct
     5401 cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc
     5461 tctgataatt agctaagtgc atgttgttca tgtcggatgc ggcgtgaacg ccttatcaaa
     5521 cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat aagcgtagcg
     5581 catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc ttcacagcac tttattaatt
     5641 ccttgaacga atatttactg ccatttcatt cacgtctatt cttaatttgc tgctttaaaa
     5701 acaagtgatt gagcaaaatt gaggcaaaaa tggactggta tctgaaagta ctaaaaaatt
     5761 atgtcggttt ccggggtcgt gcgcggcgca aagagtactg gatgtttatt ctggtcaaca
     5821 tcatctttac gttcgtgctg gggctgctgg ataaaatgtt aggctggcaa cgcgctggtg
     5881 gcgaaggcat cctgacgaca atctacggta ttctggtgtt tttaccgtgg tgggcagttc
     5941 agttccgccg cctgcacgac accgaccgct cggcgtggtg ggcactgttg ttcttaatcc
     6001 cgtttatcgg ctggcttatt atcatcgtct ttaactgcca ggcgggtacg ccaggcgaaa
     6061 accgctttgg accagatccg aaactggaac cttaaaacaa atctgctcgc cggatgtctg
     6121 actcatccgg cttttatcat catgttgatg aatgaataat ttactaatgt ttatttaaaa
     6181 tatttcaatg tcgttatata aattacctat aaaaaacaac catgtattta tcttaaatat
     6241 caaaaccttt ataacatccg aaaaagaaag atacgaatag catttacaca atacccggaa
     6301 tagactttcg tcacttttaa ttaaagggat gtttttatgc agtggtattt agccgtatta
     6361 aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag agtactggat gtttactctg
     6421 attaacgcca tcgtcggcgc tattatcaat gtcattcaat tgattttagg tttagagttt
     6481 ccattccttt ctctcattta cctggcagca acaatcattc cggtcattgc tctgtgtgtt
     6541 cgtcgtctgc acgatactga ccgttcaggt gcgtgggcgc ttctctatct ggtaccgatc
     6601 attggctggt tagttctgtt tgtatttgcc tgtttagaag gcaattctgg cagcaatcgt
     6661 tacggaaacg atcctaagtt tggttcaaat taatcttaga attgggg
//
LOCUS       DAPHDX010000192         6589 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPHDX010000192 DAPHDX010000000
VERSION     DAPHDX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.195"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            317..4570
                     /gene="fdeC"
                     /locus_tag="RH058_004142"
     CDS             317..4570
                     /gene="fdeC"
                     /locus_tag="RH058_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDV3139645.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQVYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAANTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAAIYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(4691..5548)
                     /locus_tag="RH058_004143"
     CDS             complement(4691..5548)
                     /locus_tag="RH058_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3139646.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKPTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5797..>6589
                     /locus_tag="RH058_004144"
     CDS             5797..>6589
                     /locus_tag="RH058_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV3139647.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDL"
BASE COUNT         1803 a         1469 c         1722 g         1595 t
ORIGIN      
        1 tccaatagtt gttaattaaa ggcaggccat tattattcac tgtccgcaga gtaaaaaata
       61 taacttaggt gtctatttaa tgacttgcac aaaaagctaa attttcaccc ataaataaaa
      121 atataatccc gcgcccaacc acctgatgag tggctacaga cactggatat attaggtggc
      181 ggtgcacttt cttacataaa ggtatttcct tttctgcgga aaaggaaatc gggaaatccc
      241 cggtttttct gacaagcaga cgccattatt tgtgtctgcc tatgttcgtt aattcgttca
      301 tcaggaaatt atctcaatgt cgcgttataa aacaggtcat aaacaaccac gatttcgtta
      361 ttcagttctg gcccgctgcg tggcgtgggc aaatatctct gttcaggttc tttttccact
      421 cgctgtcacc tttaccccag taatggcggc acgtgcgcag catgcggttc agccacggtt
      481 gagcatggga aatactacgg taactgctga taataacgtg gagaaaaatg tcgcgtcgtt
      541 tgccgcaaat gccgggacat ttttaagcag tcagccagat agcgatgcga cacgtaactt
      601 tattaccgga atggccacag ctaaagctaa ccaggaaata caggagtggc tcgggaaata
      661 tggtactgcg cgcgtcaaac tgaatgtcga taaagatttc tcgctgaagg attcttcgct
      721 ggaaatgctt tatccgattt atgatacgcc aacaaatatg ttgttcactc agggagcaat
      781 acatcgtacc gacgatcgta ctcagtcaaa tattggtttt ggctggcgtc atttttcagg
      841 aaatgactgg atggcggggg tgaacacctt tatcgaccat gatttatccc gtagtcatac
      901 ccgcattggt gttggtgcgg aatactggcg cgattatctg aaactgagcg ccaatggtta
      961 tattcgggct tctggctgga aaaaatcgcc ggatattgag gattatcagg aacgcccggc
     1021 gaatggttgg gatatccgcg cagagggcta tttacctgcc tggccgcagc ttggcgcaag
     1081 cctgatgtat gaacagtatt atggcgatga agtcgggctg tttggtaaag ataagcgcca
     1141 gaaagacccg catgctattt ctgccgaggt gacctatacg ccagtgcctc ttctgacact
     1201 gagcgccggg cataagcagg gcaagagcgg tgagaatgac actcgctttg gcctggaagt
     1261 taactaccga attggcgaac ctctggagaa acaactcgat acggatagca ttcgcgagcg
     1321 tcgggtactg gcaggcagcc gctatgacct ggttgagcgt aataacaaca tcgttcttga
     1381 gtaccgcaaa tctgaagtga tccgtattgc tctgcctgag cgtattgaag gtaagggcgg
     1441 tcagacactt tccctggggc ttgtggtcag caaagcaact cacggactga aaaatgtgca
     1501 gtgggaagcg ccgtcattac tggctgaagg tggcaaaatt accggtcagg gtagtcagtg
     1561 gcaagtaacg ctcccggctt atcgtccagg caaagacaat tattatgcga tttctgcggt
     1621 tgcctacgat aacaaaggca atacctcaaa acgcgtgcag acagaggtgg tcattaccgg
     1681 agctggtatg agcgccgatc gcacggcgtt aacgcttgac ggtcagagcc gtattcaaat
     1741 gcttgctaac ggtaatgagc aaagaccgct ggtgctgtct ctgcgcgacg ccgagggcca
     1801 gccagtcacg ggcatgaaag atcagatcaa gactgaacta actttcaaac cggctggaaa
     1861 tattgtgact cgtaccctga aggccactaa atcacaggca aagccaacac tgggtgagtt
     1921 caccgaaact gaagcagggg tgtatcagtc tgtctttact actggaacgc agtcaggtga
     1981 ggcaacgatt actgttagtg ttgatggcat gagcaaaacc gtcactgcag aactgcgggc
     2041 cacgatgatg gatgtggcaa actccaccct gagcgctaac gagccgtcag gtgacgtggt
     2101 tgctgatggt cagcaagtct atacgttgac gttgactgcg gtggactccg agggtaatcc
     2161 ggtgacggga gaagccagcc gcttgcgatt tgttccgcaa gacactaatg gtgtaaccgt
     2221 tggtgccatt tcggaaataa aaccaggcgt ttacagcgcc acggtttctt cgacccgtgc
     2281 cggaaacgtt gttgtgcgtg ctttcagcga gcagtatcag ctgggcacat tacaacaaac
     2341 gctgaagttt gttgccgggc cgcttgatgc agcacattcg tccatcacac tgaatcctga
     2401 taaaccggtg gttggcggta cagttacggc aatctggacg gcaaaagatg ctaatgacaa
     2461 ccctgtaact ggtctcaatc cggatgcacc gtcattatcg ggcgcagctg ctgtaggttc
     2521 tacggcatca ggctggacgg ataatggcga cgggacctgg actgcgcaga tttctctcgg
     2581 cactacggcg ggtgaattag aagttatgcc gaagctaaat ggacaggatg cggcagcaaa
     2641 tgcggcaaaa gtaaccgtgg tggctgatgc gttatcttca aaccagtcga aagtctctgt
     2701 cgcagaagat cacgtaaaag ccggcgaaag tacaaccgtg acgctggtgg cgaaagatgc
     2761 gcatggcaac gctatcagtg gtctttcgtt gtcggcaagt ttgacgggga ccgcctctga
     2821 aggggcgacc gtttccagtt ggaccgaaaa aggtgacggt tcctatgttg ctacgttaac
     2881 tacaggcgga aagacgggcg agcttcgtgt catgccgcta ttcaacggcc agccagcagc
     2941 caccgaagcc gcgcagttga cggtcatcgc cggagagatg tcatcagcga actctacgct
     3001 tgttgcggac aataaggctc cgaccgtcaa aacgacgacg gaactcacct tcaccgtgaa
     3061 ggatgcgtac gggaacccgg tcaccgggat gaagccagat gcaccagtgt ttagcggtgc
     3121 cgccaacacg gggagtgagc gtccttcagc aggaaactgg acagagaaag gtaatggggt
     3181 ctacgtgtcg accttaacgc tgggatctgc cgcgggtcag ttgtctgtga tgccgcgagt
     3241 gaacggccaa aatgccgttg ctcagccact ggtgctgaat gttgcaggtg acgcatctaa
     3301 ggctgagatt ggtgatatga cagtgaaggt taataaccaa ctggctaatg gacagtctgc
     3361 taaccagata accctgaccg tcgtggacag ctatggtaac ccgttgcagg ggcaagaagt
     3421 tacgctgaat ttaccgcagg gtgtgaccag caagacgggg aatacagtaa caaccaatgc
     3481 ggcaggtaaa gcggatattg agttgatatc aaccgttgca ggggaacttg aaatcgcggc
     3541 cgcggtgaaa aactctcaga agacggtcac ggtgaaattc aacgcggatg ccagcaccgg
     3601 tcaggcaaac ctgcaggtag acaccgccgt tcaaaaagta gcaaacggca aagatgcctt
     3661 tacgctgacg gcgaccgttg aggataaaaa tggtaaccct gttccaggga gcctggtgac
     3721 ctttaatctg ccccggggtg tcaagccgct tacaggcgat aatgtctggg tgaaagccaa
     3781 cgatgagggg aaagcagagt tgcaggtggt ttcagtgact gccggaacgt atgagatcac
     3841 ggcatcggcg gggaatagcc agccttcgaa tacgcagact ataacgtttg tagccgataa
     3901 ggctaccgca accgtctccg gtattgaggt gatgggcaac tatgcgctgg cggacggcaa
     3961 agccaaacaa acgtataaag ttacggtgac tgatgccaat aacaatttgg tgaaagatag
     4021 cgaagtgacg ctgactgcca gcccggcttc gttaaacctg gaaccgaatg gcactgcgac
     4081 aacgaatgag caagggcagg ctattttcac cgctaccact actgttgcgg cgatatacac
     4141 actcaaggcg caagtgagtc agaccaacgg tcaggtatca acgaaaactg ccgaatctaa
     4201 attcgttgcg gatgataaaa acgcggtact caccgcatca tctgatatgc aatctctggt
     4261 ggcggatggg aaatcgactg cgaagctgga ggtgacactg atgtcggcaa acaaccccgt
     4321 tggcgggaat atgtgggtcg acattcagac gccggaaggg gtgacggaga aggattatca
     4381 gttcctgccg tcgaaaaatg accatttcgt gagcggaaaa atcacgcgta aatttagtac
     4441 cagcaagcct ggtgtctata cgttcacatt taacgccctg acctatggcg ggtacgaaat
     4501 gaagccagtg acggtgacca ttaccgcggt ggatgccgat acggcaaagg acgaggaggc
     4561 gatgaaataa ttttaagaat atgaaagtaa cattctaata aacaataatg gcgttgacat
     4621 cttcaacgcc attatttatt gaatttcctg acagtagtct cgttataaat tcatttagat
     4681 atagcgaaaa ctaaaccgga cgaacgggga taaaaatttc gcaaacaaca tcattgctat
     4741 ccttttctac gaaatcttca ttgtaataaa accgctcaat atcactgccg ttcactctgg
     4801 ctaaccccct ggcgggtaat tccatcagat agaggtttcg cgcccattca gggtagtgct
     4861 cccactcacc ttcatagcgg aaagaggcat acagtcctcg gttaattgtc cactctttcg
     4921 gcatctctga atcggttggt ttgcataccg taccagcaaa agtttcaacc gtgagcaaat
     4981 cgaccgattt tgttgacggt tcaaacgtgg aaacgaaata gatatccgtc ttaaaattct
     5041 tcatacatct ggctaaatga tgacgtcgta gtttgacttc ttcatccggt gatttattcg
     5101 acacttcaat gtcatattta aatgagttgc caaaaatagg tgtctcagga aagttgatta
     5161 atctacactc cgtcttttgc tgttgacgta ttaaaaaaga agggaagata tttgcgagat
     5221 cccaataatc acggtggcgg tattcacggg gagagcagcc gaataacttt ttaaattcgc
     5281 ggctgaatga ctgctgtgaa tcaaaatgca aagagagtgc aatatcaagc atagatttcg
     5341 cggtgagccg gacaagaatg gcggcacgac aaagccttcg tttgcgaatg tattctccca
     5401 aaggcacatg catgaaattt cggaacagaa gctggatgtt gcgtctgctg tagccagatt
     5461 tctgtgcgat atcttcgatt gaaatagggt gctcaagatt gcactcaatc cactccagga
     5521 gctgctgtag aatcttctgc ctgatcatct cattctccgt taattctttg tcatatcgca
     5581 cattttcgtt ataccctggg ccctaagtgt atcaaaaata gcaagggcga aggcaaaagc
     5641 actggtagcg tctgcttcgt tgtccccaaa aatagagagg tttcagtcct acattattaa
     5701 tgaatttttt gcataagtga tatcaaaatc cacgtactaa tttgaggtta cgttttaacg
     5761 tagactcatt gttcatgcct aatggaggga ctgacagtgg aattttccgt attaagtaac
     5821 aatctgaaaa tgccgatgat gggatttggt gtttttcagg ttaccgataa aaacgtgtgc
     5881 aaacagtcag tgttgaacgc tatccgcaca ggttatcgac tcatcgacac tgcagcggta
     5941 tacggtaatg aagatgccgt cggtgaagcc gttcgtgaag ctatatctga aggtttatgt
     6001 acccgagatg agttatttat aacgtctaaa ttgtgggtgc aggatatgtt gaatcaggat
     6061 acggcagcag caggtattga agcatcatta aaaaaatcgg gactagagta cttcgacctc
     6121 tatttattgc accaggctat gcgcgattat ttcagtgcgt ggcgtgcact cgaagatgcc
     6181 tatgaagagg gcaaattaaa agcaattggg gtttccaatt tctatcctca tgttctggcg
     6241 aacttttgtg aaacggtaag agttaaaccg atggtcaacc aggtcgagtt gcatccctat
     6301 tttgcccaac cagaggcgct ggcaaccatg aagtattata acgtgcagcc tgaagcatgg
     6361 gctccgttag gtggtggacg acataaaccc tttgaaaata atctgcttca gagtattgca
     6421 gatgcccatc aaaaatcgat ttctcaagtc attctgcgtt ggaatattca acggggagtg
     6481 gtcgttattc cgaaatcgac acatcaacag cgtatcgaag aaaattttgc tatctgggat
     6541 ttctcactga cagagaaaga aatggcacaa attagttcgc ttgatttgg
//
LOCUS       DAPHDX010000193         6527 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPHDX010000193 DAPHDX010000000
VERSION     DAPHDX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.196"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..151
                     /locus_tag="RH058_004145"
     CDS             <1..151
                     /locus_tag="RH058_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139648.1"
                     /translation="ILIHVKNIIPIITATLILNYLNNSERSLVKQILIEDEIIVCATY
                     LIPDI"
     gene            153..497
                     /locus_tag="RH058_004146"
     CDS             153..497
                     /locus_tag="RH058_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV3139649.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            501..836
                     /locus_tag="RH058_004147"
     CDS             501..836
                     /locus_tag="RH058_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV3139650.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(893..3214)
                     /locus_tag="RH058_004148"
     CDS             complement(893..3214)
                     /locus_tag="RH058_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3139651.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYAIWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(3331..3540)
                     /locus_tag="RH058_004149"
     CDS             complement(3331..3540)
                     /locus_tag="RH058_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139652.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(3940..4158)
                     /locus_tag="RH058_004150"
     CDS             complement(3940..4158)
                     /locus_tag="RH058_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139653.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(4290..5813)
                     /locus_tag="RH058_004151"
     CDS             complement(4290..5813)
                     /locus_tag="RH058_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3139654.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(6145..6393)
                     /locus_tag="RH058_004152"
     CDS             complement(6145..6393)
                     /locus_tag="RH058_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139655.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         1867 a         1481 c         1372 g         1807 t
ORIGIN      
        1 tattctcatt cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta
       61 tctgaataat agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt
      121 gtgtgcaact tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc
      181 ttatctcagc attgttctgc tccacgccag cgatggcgga tatgaatcgc accacgaaag
      241 gtgcattgct aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga
      301 aaggtgcagc tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa
      361 atgcgcgtaa aggtgcaaaa gtaggtgccg ccgttggtgc tgtcactggc gttctgaccg
      421 gcaatggcct cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc
      481 tgggtaaaat gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg
      541 ttttcgcacc aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa
      601 cctatattac ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag
      661 aaattgttgc ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat
      721 ctccggaact gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc
      781 aaatgctgtt gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt
      841 tcatatgtac cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact
      901 tatatttcag gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc
      961 ccacattccc ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt
     1021 cccccaaatt actcgcccca tcttgttcga ctttcacacc gttcattgag acagcaaaat
     1081 ctttactgtt atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac
     1141 cgctgttttt ctcctgagta cgaataaagg tgcggaaacc aagtcgggtc tggatgttgt
     1201 tatttgcgtc attctcaata cgtgagccat tggcttcggt gtgcgcatta tgatcgacgc
     1261 ccatccagat agcctgtgcc tgtggctgga atatccacgt gtatttcgcc gcttccagac
     1321 caaaggattt attcagggca aagccgtaac ccgcttccag cgagacggtt gcgcctttcg
     1381 cactatagga ttcataggct aattcatccc ctttcaccga gtttttaaac cagctatatt
     1441 gcgcccaggc gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caaatggcat
     1501 acagccctgc gcaatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat
     1561 gagttgagtt gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactgt
     1621 cggtaaaacc accggttaac agctcggccc ctaactgcga gacgtagcga tgcgaggtcg
     1681 ttctcaactg tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa
     1741 gtcggcttga acgctcctgt tcagttacag gatcgatgta gcgcatttca cccgcacggt
     1801 cgttcagacg cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag
     1861 cttcgaggcg caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg
     1921 tttcaccgcc gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg
     1981 tattacctcg ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa
     2041 attccccatt tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc
     2101 cgctgcctcc tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa
     2161 cgactttatc cgtagctgaa ttatcatcac ccaattcagt tctgagatgc aatacgccat
     2221 tattaccaac gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg
     2281 cccgggaaat atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt
     2341 taatatccca ggtactatta tctgaaagcg acagataggt atgagtgtta acatctgttg
     2401 aaatattagc tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa
     2461 tattattgat aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac
     2521 tcgtctcatt tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa
     2581 tatctaccgt tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat
     2641 gcgcgactgc agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcagtcg
     2701 cggcgtcagg acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg
     2761 cagcgatggc atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc
     2821 tggcattggt ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg
     2881 cataactttc tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg
     2941 cattattttc attacttcct gtatcggtag tactttcttc atcaagagaa gatacacacg
     3001 cacgcgcgct tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa
     3061 caataaatgt atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc
     3121 ttattatggt gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat
     3181 cccccgttgt gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta
     3241 cgattgcgct agctgttaat gctgtgctta gaggactatt gtcagaagga tataaaccgc
     3301 ccgttgttac aatgacgcca ttgttaatgg ttaacttgct accctctcct gcaacggata
     3361 cccgtaactt ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc
     3421 atcttttatt gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc
     3481 attacccacc gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat
     3541 agattaacca tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca
     3601 gattaccaaa acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac
     3661 agcgacaata tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga
     3721 cattgatcac tttgaggtga cgattttgtt taacaaataa ctcttcgaca tcacaatcat
     3781 tgatagttgc aataaaaatt atttcaatga catataaaaa caactgtgat aacaccaggg
     3841 ttgacaacta tttgcgcaat aattccttta cttcaatgat ccaggcattc actgccagca
     3901 acccattcag ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct
     3961 gacgagtctt tcccttaatc gattaaccag attgatcgcc agccaaacaa gggcattaag
     4021 caaatttatc gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac
     4081 caggagggtg atcagcataa atgcaatgga aaagatgata tagagagtga aataatccag
     4141 attattacta ttgttcatgc tactaatggc aatcctgtca aaactttcaa acaattatag
     4201 caaacaaaat aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta
     4261 catcgcacta tggccttact gattaatttt cactcaacca caaccttcac cttcggttta
     4321 gaaaggataa ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc
     4381 tgaaatgtga tattattttg gttcagatag tcaagttgtt ctttcgtttc gacaccttca
     4441 gcgacgatac tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt
     4501 gattctgggt ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata
     4561 tgcaggtcat gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg
     4621 acaaaaccat tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat
     4681 ggctcacgct cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg
     4741 aaatttaaac actcgtcgac aaacgtcggc gaaataatat gcgaagcgct aaaattaatt
     4801 ccaatatgaa aaccttccgg caatttactc gcgatagcgt tcatctgtct ggcaacctga
     4861 ttcatcaggc tttgcgtaag cggaacgatt aatccggatt tttcagcaag tggaataaat
     4921 gccgcgggtg atatatatcc accgtgaggt tgtttccagc gggctaacac ctcaactccc
     4981 cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa aagggacgat ctccccatta
     5041 tttattgccc gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata tctatcaagc
     5101 aggaaggcag cggcgcaggc gattaacaga ataaatatta aaatcccaaa gcctttatcg
     5161 ataagtctgt agaagctaaa gagtggcggc gggttaaaga taatagtgaa atgatatttt
     5221 gtggagtgaa ccttctcggt atagggataa tgtccattaa gcgtcatcac atcacccgtt
     5281 ggtccaataa tgtcatccgc cacgcgtaat acatacctta cccctttcaa gggtacatta
     5341 agtgccccac gaatatgctg atcgcttatc gtaaccaaaa tgcgcgtatc tgcaaattgg
     5401 ttctgataga gcaggatagg taatctatta acggtgtcga ttgctggagc caacagtaaa
     5461 ttactgtccg ggaaaacagg aatacgagac aacaggaccc gattcccagg cagggatgta
     5521 caccagacta ttccctgttt gagaatgatg attgtgcgca ggtgaggttt cagagctgct
     5581 tcagtgccaa gctgatattg ctcctcgagg ttgcattcct tcccggcaat gttaaccgct
     5641 gtttgagtcg cttgtgacgc ttcttcaagt atatgattga tattgttggc ggcatatctc
     5701 gctcccgcca ggtactctgc ttttgcggaa taccagagct ggatgttcaa aatagcgaca
     5761 ccggtaataa acaagcatat cgcaacgagg atgggtatga gtgtattgcg catttgcttg
     5821 tcccggccaa agggtttaat acatgaaaat gccattcatt cgctcgcgct ggcatctaca
     5881 gagagcatgg tagagagcat aagaaatatc cacgccgaag ataaaatttt ctccatcttg
     5941 atctaaccca ttaattattg agcataatgt aggcagtaca aaataagtta ggcgggatat
     6001 caggcgtcaa gaatggagag cgagaactct ccattcttga cacctgatat tgcggacata
     6061 ataagaaagc ataacgcctg aaatgctcac tttgcatcag catggtgata cagctgatgt
     6121 ttattctaaa accttactca agttctaaga gagcacggat tccctgtcat taataatgat
     6181 atccgttagt gtagtcttat gcatttcaat gtactgcaat atttgcagat agagatcaga
     6241 aagaatcaca ctggtttcat cttttaaacg caagcgtaaa gcctgatcaa taatatgact
     6301 atcaacatac ccatgcgtca tgaacacctc cctctcgaga tttttaaaca aagcacattg
     6361 aaaaataaat ctctctggga ttgaattatt catgtcagcc tcgtagaaat attaacccaa
     6421 taaaattatg ctgtgtatag tctaaagcga cagacaaaaa taagatgaat gagttttcta
     6481 taaacttata cttaataatt agaagttaca tatcatcagc tgtgtat
//
LOCUS       DAPHDX010000194         6472 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPHDX010000194 DAPHDX010000000
VERSION     DAPHDX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.197"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..1015
                     /gene="iaaA"
                     /locus_tag="RH058_004153"
     CDS             50..1015
                     /gene="iaaA"
                     /locus_tag="RH058_004153"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV3139656.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            1002..2873
                     /gene="gsiA"
                     /locus_tag="RH058_004154"
     CDS             1002..2873
                     /gene="gsiA"
                     /locus_tag="RH058_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV3139657.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2893..4431
                     /gene="gsiB"
                     /locus_tag="RH058_004155"
     CDS             2893..4431
                     /gene="gsiB"
                     /locus_tag="RH058_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV3139658.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPTTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4449..5369
                     /gene="gsiC"
                     /locus_tag="RH058_004156"
     CDS             4449..5369
                     /gene="gsiC"
                     /locus_tag="RH058_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV3139659.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5372..6283
                     /gene="gsiD"
                     /locus_tag="RH058_004157"
     CDS             5372..6283
                     /gene="gsiD"
                     /locus_tag="RH058_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV3139660.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1434 a         1558 c         1894 g         1586 t
ORIGIN      
        1 tctgatattt atagcaaaag tggcgagcca cccttaatgg acgaatacta tgggcaaagc
       61 agtcattgca attcatggtg gcgcaggtgc aattagccgc gcgcagatga gtctgcaaca
      121 ggaattacgc tacattgagg cgttgtctgc cattgttgaa accgggcaga aaatgctgga
      181 agcgggcgaa agtgcgctgg atgtggtgac ggaagcggtg cgtctgctgg aagagtgtcc
      241 actgtttaac gccggaattg gcgctgtctt tacgcgtgat gaaacccatg aactggacgc
      301 ctgtgtgatg gatggtaaca ccctgaaagc cggtgcggtg gcgggcgtta gtcatctgcg
      361 taatccggtt cttgccgccc ggctggtgat ggagcaaagc ccgcatgtga tgatgattgg
      421 cgaaggggca gaaaattttg cgtttgctcg tggcatggag cgcgtctcgc cggagatttt
      481 ctccacgcct ttgcgttatg aacaactact ggcagcgcgc aaggaagggg caaccgtcct
      541 cgaccatagc ggtgcgccac tggatgaaaa acagaaaatg ggcaccgtgg gggccgtggc
      601 gttggattta gacggcaatt tggcggcagc cacgtccaca ggcggaatga ccaataaatt
      661 acccggacga gttggcgata gtcccttagt gggtgccgga tgctacgcca ataacgccag
      721 tgtggcggtt tcttgtaccg gcacgggcga agtcttcatc cgcgcgctgg cggcatatga
      781 catcgccgcg ttaatggatt acggcggatt aagtctcgcg gaagcctgcg agcgggtagt
      841 aatggaaaaa ctccctgcgc ttggcggtag cggtggctta atcgctatcg accatgaagg
      901 gaatgtcgcg ctaccgttta acaccgaagg aatgtatcgc gcctggggct acgcaggcga
      961 tacgccaacc accggtatct accgtgaaaa aggggacacc gttgccacac agtgatgaac
     1021 ttgatgccgg taatgtgctg gcggttgaaa atctgaatat tgcctttatg caggaccagc
     1081 agaaaatagc tgcggtccgc aatctctctt ttagtctgca acgcggtgag acgctggcaa
     1141 ttgttggcga atccggctcc ggtaagtcag tgactgcgtt ggcattgatg cgcctgttgg
     1201 aacaggcggg cggtttagta cagtgcgata aaatgctgtt gcagcggcgc agtcgcgaag
     1261 tgattgaact tagcgagcag agcgctgcac aaatgcgcca tgttcgcggt gcggatatgg
     1321 cgatgatatt tcaggagccg atgacatcgc tgaacccggt atttactgtg ggtgaacaga
     1381 ttgccgaatc aattcgtctg catcagaacg ccagtcgtga agaagcgatg gtcgaggcga
     1441 agcggatgct ggatcaggta cgcattcctg aggcacaaac cattctttca cgttatccgc
     1501 atcaactctc tggcgggatg cgccagcgag tgatgattgc gatggcgctg tcatgccgcc
     1561 cggcggtgct gattgccgat gagccaacca ccgcgctgga tgtcactatt caggcgcaga
     1621 tcctgcaatt aatcaaagta ttgcaaaaag agatgtcgat gggcgttatc tttatcactc
     1681 acgatatggg cgtggtggca gagattgccg atcgggtact ggtgatgtat cagggcgagg
     1741 cggtggaaac gggtaccgtc gaacagattt ttcatgcacc gcaacatcct tacacccgtg
     1801 cgctgttagc tgctgttccg caacttggtg cgatgaaagg gttagattat ccccgacgtt
     1861 tcccgttgat atcgcttgaa catccagcga aacaggcccc ccccatcgag cagaaaacgg
     1921 tggtggatgg cgaacctgtt ttacgagtgc gtaatcttgt cacccgtttc cctttgcgca
     1981 gcggtttgtt gaatcgcgta acgcgggaag tgcatgccgt tgagaaagtc agttttgatc
     2041 tctggcctgg cgaaacgcta tcgctggtgg gcgagtctgg cagcggtaaa tccactaccg
     2101 ggcgggcgtt gctgcgcctg gtcgaatcgc agggcggcga aattatcttt aacggtcaga
     2161 gaatcgatac cttgtcaccc ggcaaacttc aggcattgcg ccgggatatt cagtttattt
     2221 ttcaggaccc ttacgcttcg ctggacccac gtcagaccat cggtgattcg attatcgaac
     2281 cgctgcgtgt acacggttta ttgccaggta aagacgcggc tgcacgcgtt gcgtggttgc
     2341 tggagcgcgt gggcctgtta cctgaacatg cctggcgtta cccgcatgag ttttccggcg
     2401 gtcagcgcca gcgcatctgc attgctcgcg cgttggcatt gaatccaaaa gtgatcattg
     2461 ccgacgaagc cgtttcggcg ctggatgttt ctattcgcgg gcagattatc aacttgttgc
     2521 tcgatctcca gcgtgatttc ggcattgcgt atctgtttat ctcccacgat atggccgtgg
     2581 tagagcggat tagtcatcgt gtggcggtga tgtatctcgg gcaaattgtt gaaattggtc
     2641 cacggcgcgc ggtcttcgaa aacccgcagc atccttatac gcgtaaatta ctggcggcag
     2701 ttccggtcgc tgaaccgtcc cgacaacgac cgcagcgtgt actgctgtcg gacgatcttc
     2761 ccagcaatat tcatctgcgt ggcgaagagg tggcagccgt ctcgttgcaa tgcgtcgggc
     2821 cggggcatta cgtcgcacaa ccacaatcag aatacgcatt catgcgtaga taacattcag
     2881 gcggagaata aaatggcaag agctgtacac cgtagtgggt tagtggcgct gggcattgcg
     2941 acagcgttga tggcatcttg tgcattcgct gccaaagatg tggtggtggc ggtaggatcg
     3001 aatttcacca cgctcgatcc gtatgacgcg aatgacacgt tatctcaggc cgtagcgaaa
     3061 tcgttttacc aggggctgtt cggtctggat aaagagatga aactgaaaaa cgtgctggcg
     3121 gagagttata ccgtttccga tgacggcatt acttacaccg tgaaattgcg ggaaggcatt
     3181 aaattccagg atggcaccga tttcaacgcc gcggcggtga aagcgaatct ggaccgggcc
     3241 agcgatccgg cgaatcatct taaacgctat aacctgtata agaatattgc caaaacggag
     3301 gcgatcgatc caacaacagt aaagattacc ctcaaacagc cgttctcagc gtttattaat
     3361 attcttgccc atccggcgac cgcgatgatt tcaccggcag cgctggaaaa atatggcaag
     3421 gagattggtt ttcatccggt gggaaccgga ccgtatgaac tggatacctg gaatcagacc
     3481 gattttgtga aggtgaaaaa attcgcgggt tactggcagc caggattgcc caaactggac
     3541 agcataacct ggcgtccggt ggcggataac aacacccgcg cggcaatgct gcaaaccggt
     3601 gaagcgcagt ttgctttccc cattccctac gagcaggcca cactgctgga gaaaaacaaa
     3661 aatatcgagt tgatggccag tccgtcaatt atgcagcgtt atatcagtat gaacgtgacg
     3721 caaaagccgt tcgataaccc gaaggtccgt gaggcgctga attacgccat taaccgtccg
     3781 gcgctggtga aagttgcctt tgcgggctat gcaacgccag ctactggtgt ggtaccgcca
     3841 agtatcgcct acgcgcaaag ttataaaccg tggccttacg atccagtgaa agcgcgcgaa
     3901 ttactgaaag aggcgggata tcccaacggt ttcagtacca cgctgtggtc gtcacataac
     3961 cacagcaccg cgcagaaagt gctgcaattt acccagcagc agttagcgca ggtcgggatt
     4021 aaagcccagg tgaccgcgat ggatgccgga cagcgggcgg cagaagttga aggtaaaggg
     4081 caaaaagaga gcggcgtgcg gatgttctac actggctggt cggcttcaac cggcgaagcg
     4141 gactgggcgc tatcgccact gttcgcctcg caaaactggc caacgacgct gtttaatacc
     4201 gcattttaca gcaataaaca ggtggacgac ttcctggctc aggcactgaa aacgaatgat
     4261 ccggcggaaa agacccgctt gtataaggcg gcgcaggata tcatctggca agagtcgccg
     4321 tggatcccgc tggtggtaga aaaactggtg tcggcacaca gtaaaaacct gaccggtttt
     4381 tggatcatgc cagacaccgg cttcagcttt gaagacgcgg atttgcaata agcaacgcag
     4441 ggagtggaat gcttaattac gttatcaaac gattactggg gttgattccg acgctgttta
     4501 tcgtctcggt gctggtgttt ttatttgtcc acatgctgcc cggcgatccg gcgcgattga
     4561 ttgccgggcc cgaagctgat gcgcaggtta tagaactggt gcgtcagcag ctggggttgg
     4621 atcagccgct gtatcaccag ttctggcact atatcagcaa tgctgtgcag ggggattttg
     4681 gcctgtcgat ggtgtcgcgt cgtccggttg ccgatgagat tgccagccgc tttatgccaa
     4741 cgctgtggct gaccataacc agtatggtct gggcggtgat atttggtatg gcggcgggaa
     4801 ttatcgccgc cgtctggcgt aatcgttggc cggatcgatt gagtatgacc attgcggtgt
     4861 cggggatctc gtttccggca tttgcgctgg ggatgctttt aattcaggta ttctccgttg
     4921 aactgggctg gctgcctacc gtgggagcag acagttggca gcactacatt ttaccctccc
     4981 tgacgctcgg cgcggcagtg gcagccgtga tggcgcgctt tacccgcgcg tcgtttgtcg
     5041 atgttttaag cgaagattat atgcgtaccg cgagggcgaa aggggtgagc gaaacctggg
     5101 ttgtcctcaa acacgggcta cgcaacgcga tgatcccggt agtgaccatg atgggcttac
     5161 agtttggctt tttgctcggt ggttctatcg tggtggagaa agttttcaac tggcccggac
     5221 ttggacgctt actcgttgac tccgtagaaa tgcgcgatta cccggtgatt caggcggaaa
     5281 ttctgctttt ctcactggaa tttattctta tcaacttagt ggtggatgtg ctttacgccg
     5341 ccattaaccc ggctatcagg tacaagtaag gatgcgacta tttaactggc gacgtcaggc
     5401 ggtgttaaac gccatgccac tggtcaaacc tgaccaggta cgtacaccgt ggcatgaatt
     5461 ctggcgacga tttcgccgtc agcatatggc gatgaccgcc gcattattcg ttattttatt
     5521 gattgtggtg gccatttttg cacgctggat cgctccctat gacgccgaaa attattttga
     5581 ttatgacaat ctgaataacg gaccttcttt gcagcactgg tttggcgtcg attcactggg
     5641 gcgcgacatt ttcagccgtg tcctggttgg tgcgcaaatc tcgctggcgg cgggcgtgtt
     5701 tgccgtgttt atcggtgcgg cgatagggac gttgctgggc ttgctggctg gatattatga
     5761 aggctggtgg gatcggctga tcatgcgcat ttgcgatgtg ctgtttgcct tcccgggtat
     5821 tttactggcg atcgctgttg ttgcggtgtt gggaagcggc attgctaacg tgattatcgc
     5881 agtcgccatt ttttccatcc ccgcgtttgc ccgcctggtg cgcggcaaca cgctggtgtt
     5941 gaaacagcaa acctttattg agtcagcacg cagtattggt gccagcgata tgaccatttt
     6001 attgcgtcat atcctgcctg ggaccgtctc gtctatcgtg gtgtttttca ccatgcgcat
     6061 tggcacctcg attatctctg ccgccagcct gtcatttctc ggcctcggtg cgcagccgcc
     6121 gacaccagag tggggagcaa tgctcaatga ggcacgagcg gatatggtta tcgcgccgca
     6181 tgtcgctgtt tttccggccc tggctatttt tctgaccgta ctggcgttca atttgttggg
     6241 cgatggttta cgcgatgcgc tggatccgaa aattaaagga tagttacgtt tgaatattgc
     6301 ttgaaagggt aatcacctca caggaaatta ttgccctaag caagtgttgt aactttctgc
     6361 tgattttgta gaatcgggta atttggttaa aaagccgcag caagggacta tttttgcagc
     6421 ggcacagcgt tcagacagtt attttgttaa atgtattaac atgctgagtt ta
//
LOCUS       DAPHDX010000195         6454 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPHDX010000195 DAPHDX010000000
VERSION     DAPHDX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.198"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(361..1029)
                     /locus_tag="RH058_004158"
     CDS             complement(361..1029)
                     /locus_tag="RH058_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDV3139661.1"
                     /translation="MQSVYAIVTVNLYSYHGHHKMKKVILFLCQGLEEYEASVFTDTI
                     GWTTTYGLEPIELVTVGLRQKVKCAWNFTIEPEYQLHEIDDDGFDAIAIPGGMSRAGF
                     YEDAFDERLLRLIQKFNAKNKIIASVCVGALAIAKSGALQGRTGTTYHLSSKRQAQLL
                     EMGVHVVQHPIVVDGNIITSQSPATAINVAFTLIEKLTSRANVERIKEGMGFAPLSID
                     PSGM"
     gene            complement(1235..1726)
                     /locus_tag="RH058_004159"
     CDS             complement(1235..1726)
                     /locus_tag="RH058_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014838325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HDV3139662.1"
                     /translation="MDTDKLVTLFRYKRWIDIETLQAIKRIDTSVNAEKHHLTLRLMN
                     HIHVVDSIFRANVTGQKHSYTALNTPETPSADELLITMTECTDWYIQHVNSMSSAELA
                     ETIKFQFVDGGHGEMKAFDMLNHVLFHGTYHRGAVGWLISESGVVPPKDVLTVFLRDH
                     NHE"
     gene            1964..2437
                     /locus_tag="RH058_004160"
     CDS             1964..2437
                     /locus_tag="RH058_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010670508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDV3139663.1"
                     /translation="MRIRPSSRLIILSPENHVLLFRFCHKDDALRGKTYWATPGGGLE
                     KNESFEQAAIRELFEETGLTRTLAGPQIASRTFTMMLPSGETVLAEERFFMINANTVD
                     LDRSGWSSNEKEVIRDHHWWTIEELIHTNETIFPRDLIISILENKSAGSSTLTAS"
     gene            complement(2473..3267)
                     /locus_tag="RH058_004161"
     CDS             complement(2473..3267)
                     /locus_tag="RH058_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3139664.1"
                     /translation="MRNVKIEDVDKLDREVVALGNDYAQGFILPQHQHRRAQLLYGAT
                     GLMYVSTCNGEWVVPPQHAVWIPPETPHAVRFVGVTTRSLYIEPTCVTGSIEYRHCKV
                     ISVSPLLRQLLLDAVDLPLMYDSARDRALVNLLLLELAEMPVREFDIPLPQHPALLAL
                     CQAFLLTPSIHDPAERWASTLFMSASTFRRLFLRQTGMSFSAWRQRACVVSALTLLIT
                     GKPVNEVALTLGYDNASSFATMFRRVTGQPPSYCRLDFKSFTGHRS"
     gene            3377..4168
                     /locus_tag="RH058_004162"
     CDS             3377..4168
                     /locus_tag="RH058_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV3139665.1"
                     /translation="MNDIAIIALAGFTTGITTVLFGFGGGFVVVPFVYQLMLRQSELA
                     GNAMHIAVATSTAVMIFNAGWVSYRNWRAGKLSAQTLFPLLWFIAIGAVVGSCLAGVF
                     SENIVRALFILYMLVTIGDCLLRKGFFICSSPRRLSLSVVTGGGVIIGMIAALLGVGG
                     SVMTVPLLRRHGYAMGECISASNPLSLPVALCGAVTYAVIGWQNIPVSGFLGFISLKI
                     LGLLVLTGWAGIVFSRRVIPALPDIWHARIYVILLCLVLLAMLLQ"
     gene            complement(4560..5462)
                     /locus_tag="RH058_004163"
     CDS             complement(4560..5462)
                     /locus_tag="RH058_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139666.1"
                     /translation="MDLFPDIEQAVPLPHEEVLRYMVCKICDNRSEEWLTEMCRKNNL
                     SDVCQDYLQKGVLKALEGKPDVSFYPPRLTIELVPRTCWFDNVRSAVSSTDWKRLRQQ
                     TARTAGWKCQVCGGKGPRWPVECHEIWHYDDDHQYQTLKGLIALCPSCHEVKHMGFSE
                     LRGKKDEAVAHLALVNGWSLQGAFDYVDEAFDVWRARSRHAWQLDISWLETHGIKLKD
                     ETAAEGDNIPPENQLSLELEVPPVAMESTAVSAVVEQESNSCEMGQILKDKTVSRSSS
                     AWPAFWSVCQRVFYRVKKVIREQW"
     gene            5909..6115
                     /locus_tag="RH058_004164"
     CDS             5909..6115
                     /locus_tag="RH058_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3139667.1"
                     /translation="MNNQPTRLIRLPEVIARTGYGKAWIYHLISKGRFPQPVKIGLRA
                     IAFVESEVEEWIQSVIETTRKNVA"
     gene            6130..6426
                     /locus_tag="RH058_004165"
     CDS             6130..6426
                     /locus_tag="RH058_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139668.1"
                     /translation="MNIECFVSENHMCNLGERLFNEIFDLDAIAELADDKFTREAVYM
                     LSQYLVGNTSGPFKAINMLGAHEQIHIYEIWISIIKSADELRILFKAILRHCIE"
BASE COUNT         1697 a         1510 c         1501 g         1746 t
ORIGIN      
        1 aatagaatgg ataatgacaa aatagtagta tatttaaaaa catgcagtgc agtatgatgt
       61 ttcaaaatta cttgttcgag gaaaagaaaa aatctccctt tttactgtac tttattcagt
      121 tgaggtacaa ctttgtcgag tagttaactt agggaaggac aagttctaca gcagattaag
      181 aataactaac ggcaactata gtacttatca aaagcttcaa atgccctaga gactgcccat
      241 gtttgagaag tgctgatctg ctccccttaa acgaatgcag catgacgtta ggaacttccg
      301 ctcttggcac taagtggaaa acctcttgga acagaaggtc tgtaatgagc gagaagagga
      361 ctacattccg gatggatcga tagagagggg ggcaaatccc ataccttctt ttattcgttc
      421 aacattagct ctagatgtca gcttttctat taaagtaaat gctacattaa tagctgttgc
      481 cggactttga gacgtaataa tatttccgtc caccactata gggtgctgaa caacatggac
      541 tcccatttct agcaactgag cttgacgttt actgctcaga tgatacgttg tgcccgttct
      601 tccttgtaaa gcgccactct tggcaattgc taaggcacct acgcaaacag aagcaataat
      661 cttgtttttt gcgttaaatt tttgaataag ccttagaagg cgctcatcaa atgcatcttc
      721 ataaaaaccg gcccgactca tccccccagg aatggctatg gcgtcaaagc catcatcatc
      781 aatttcatgt agttgatatt ctggttcgat agtgaaattc caggcgcatt taactttttg
      841 acgtagacca actgtcacta attctatggg ttctaatccg tatgttgttg tccagccaat
      901 agtatctgtg aatacactag cctcatattc ttctaatcct tggcaaagga ataagataac
      961 tttcttcatc ttatgatgtc catggtaact atataaattc acagtaacga tagcataaac
     1021 actctgcatt tttaacctgt tgtcggtacg ggtttaatct accacttgag agactatgga
     1081 gtgaatttcc gcttttggca cacagcagac aagcacgtga cactgaagga ctactttgag
     1141 cgagaagcgg acactccatg acattcattt atgcacttac ttttttgtgt aagggttcaa
     1201 tggagttata aatttgcatg cccctgccag tcgtttattc gtggttatga tctctaagaa
     1261 aaacggtcag tacgtccttg ggcgggacta caccactttc tgaaattagc caacctactg
     1321 cgccacgatg atatgtcccg tgaaaaagca catgattgag catatcgaac gccttcattt
     1381 caccatgccc gccatcaaca aactggaatt tgattgtttc agcaagctca gcggaagaca
     1441 tggaattgac atgttgaata taccaatctg tacattcagt catcgttatc agaagctcat
     1501 cagcagatgg ggtctctggc gtgttcagtg cagtgtagga atgcttctga cctgtaacgt
     1561 tagctctgaa gatcgagtca accacatgta tgtgattcat taatctgagc gtcaggtggt
     1621 gcttttcggc atttacagac gtatcgattc ttttgatcgc ttgtagtgtt tcaatatcta
     1681 tccagcgctt gtatctaaac agcgtaacta acttgtcagt atccattcac cacctctttt
     1741 atacatttta ttatcatatc agcagcataa aaggttctct acttacttcc gcttttggca
     1801 ctgaacggac agaaactagg tactcaggtc cgctgtgagc gaagagcgga cgttgagtgg
     1861 gattcaagct ctcagaaaat gcagaccttg agggcgattc aggctgcaat ttatgtttat
     1921 ataaatatcg agattagtat ttgagtcttt ttaagggggg tatgtgcgca ttcgaccatc
     1981 atctcgattg atcattcttt cgcctgaaaa tcacgtattg ctctttaggt tctgtcataa
     2041 agatgatgct ctgaggggaa aaacatactg ggcaacgccg ggtggagggc ttgaaaaaaa
     2101 tgaatctttc gagcaggccg ctatacgcga attatttgaa gaaacgggtt tgacaagaac
     2161 attagcagga ccacaaatag cgtcccgaac cttcacaatg atgctaccaa gtggggagac
     2221 cgtacttgct gaagagcgtt tctttatgat taatgcaaat acagtagatc tcgaccgctc
     2281 agggtggagt agcaatgaaa aggaagtcat tcgcgatcat cattggtgga caatagaaga
     2341 gctcatacac accaatgaga ctattttccc acgcgattta atcattagca tacttgaaaa
     2401 caagtcagcc ggatcctcga cgttaactgc atcatgatat cccgaacgtc agcttccggc
     2461 acataactga cgttatgaac ggtgccctgt gaagcttttg aaatcaagcc gacaatacga
     2521 aggcggctgt cccgtgacac ggcggaacat agtggcgaaa gatgatgcat tgtcgtatcc
     2581 gagagtgagt gccacttcat tcaccggttt acctgttatc agcaacgtca gtgcgctgac
     2641 gacacaggct cgctgtcgcc atgcagaaaa tgacatgccg gtttgccgta agaaaagtcg
     2701 acgaaaggta ctggcgctca taaacagcgt actggcccag cgctcagcag ggtcatggat
     2761 tgagggggtg agtaaaaatg cctgacaaag agccagcaga gccggatgtt gtggcagcgg
     2821 aatatcgaat tcgcgaaccg gcatttccgc cagttccagt aacagcagat ttaccagcgc
     2881 acggtcgcga gcgctgtcat acatcagcgg caaatccacg gcatcaagca acaactgacg
     2941 taacaatggc gatacgctga tcaccttaca gtgtcgatac tcaatcgatc ccgtcacgca
     3001 tgttggttca atatataagc tacgcgtggt cacacctaca aaccggacag cgtgaggggt
     3061 ttcgggtggg atccatactg catgttgtgg gggaacaacc cactctccgt tacaggttga
     3121 gacgtacatg agacccgttg cgccatacag cagctgagcg cggcgatgtt ggtgttgtgg
     3181 cagtataaat ccctgcgcat agtcattgcc taacgccacc acttcacgat cgagcttatc
     3241 gacatcttca atttttacgt tgcgcacacc ggtctccgtc tgaacgattt acaaaggtaa
     3301 ttgagcgctg cgcaaatggg aagcaggatg ctaccggtat aagctgctcg ctcattttca
     3361 ggtcggggat attctcttga acgatatcgc aattattgca ctggcaggct ttactaccgg
     3421 tatcaccacg gtgctctttg gttttggggg cggttttgtc gtggtgccat ttgtttatca
     3481 actgatgctg cggcagtctg agctggcagg aaacgccatg catatcgcgg ttgctacctc
     3541 aacggcggtg atgatattta acgcgggctg ggtcagctat cgaaactggc gcgccggcaa
     3601 actttcagcg cagacattgt tcccattatt atggtttatc gccatcgggg ctgtcgtggg
     3661 ttcctgtctg gcgggagtct tcagcgagaa tattgttcgc gcactgttca ttctttatat
     3721 gctggtaacc attggcgatt gtttgttgcg caaaggtttt ttcatctgca gttccccgcg
     3781 tcgcttgtca ctttctgtgg taacgggcgg cggagtgatc atcggcatga tagccgcatt
     3841 gctgggcgtg ggtggcagcg tgatgacagt gccattatta cggcgacacg gctatgcaat
     3901 gggtgagtgt attagcgctt ctaacccgct ttctctgcct gtcgcgctgt gtggtgccgt
     3961 gacgtatgca gtcattggtt ggcaaaatat tccggtgagc ggatttctcg gttttatcag
     4021 cctgaaaatt ttaggcctgt tggtactgac gggctgggct ggaatagttt ttagccgtcg
     4081 ggttatacct gctttacctg acatatggca cgcacgaatc tatgtaatac tgctgtgcct
     4141 ggtgctactg gcgatgctgc tccagtaaac ccatccatcg cggcgactac tgggctacct
     4201 gtaccaacgt ccgctcctgg cacaaagcgg acactcattc caacatacaa aaatcaatga
     4261 catatctcct gtacaagctt ttcccttcca ttgccaacaa ccaaaccgtt ggcccggagg
     4321 cagcactggt ggaaattacg gcgtgctctg acccgctatg attctgaaat acgctacagt
     4381 ctcatcatct ctatccatgt aacggagacc ggtgtcgatc tgttgacgcc ggtacagagt
     4441 aaagtggata cacttatcgc cagtcagatc gaaataacat catgaaaaca gcgatgcatt
     4501 ttgatccggc tctgaactca ttcgcctctc tcaccgggaa tgcatcgcca cgggtttatt
     4561 caccactgct cccgtatcac ctttttcacc cggtagaaca ctcgctggca gactgaccag
     4621 aacgctggcc atgcggacga tgagcgggat acagttttgt ccttcaggat ttgccccatc
     4681 tcgcaagaat tgctttcttg ttccacgacg gcggacactg cagtgctctc catcgcgaca
     4741 ggcggaacct caagctccag tgaaagctga ttttcaggag gaatgttgtc gccctctgct
     4801 gctgtctcat ccttgagttt aatcccatgc gtttcgagcc acgagatatc cagttgccag
     4861 gcatgacgag atcgtgcccg ccatacgtca aaagcttcat ccacatagtc aaatgcgccc
     4921 tgaagagacc agccgttgac cagagccaga tgagccaccg cttcgtcctt tttaccccgg
     4981 agctcgctga accccatatg tttgacttca tggcatgaag ggcataacgc gatgagccct
     5041 ttgagggtct gatactgatg gtcatcatcg taatgccaga tttcatggca ctctacaggc
     5101 catcggggcc cctttccgcc gcagacctgg catttccacc ctgctgttct ggccgtttgt
     5161 tgccgtaacc gtttccagtc cgttgaagat accgcacttc tgacgttatc gaaccagcat
     5221 gttcggggca ccagctcaat cgtcagtctt ggtgggtaaa agctcacatc aggtttaccc
     5281 tccagagcct tcagcactcc cttttgaaga taatcctgac agacatcaga aaggttattt
     5341 ttcctgcaca tctctgtaag ccattcttcc gaccggttat cacagatttt acataccatg
     5401 tagcgaagca cttcttcatg gggtaaaggt acagcctgtt caatatcagg aaaaagatcc
     5461 acgcagaaat ccttttgcag aaggcgtcac acctcattat acgaaaggct ttgagctgat
     5521 taaacgtggt aaagccgcgc tcttcgtcct tagtcatcgt gcatttagtg ccagtgaatg
     5581 gccaattgga gcacccaact gcaagccaca ataaatacct gaaaagacgt aaaagcgaat
     5641 gcgacagtgt cttggtcaac taaatttggc cttagtactg gcggtataac tggttaggat
     5701 taaaaaagtt gttaatgtcc tacggtcacc cgatcacggg tagctcaact ctactggcac
     5761 tgttcagaac tggggaagtt caggcaacgt gaaattcccc gactctgggc agcaccaacg
     5821 tcgataacct ccgtctcagg agcttcactc ctttcttttc tcgaagattt gtatagtagc
     5881 cccatcgcaa cgacaagggg aaaacaaaat gaacaatcaa ccaacccgtc tcatccgtct
     5941 accagaagtc atcgctcgaa ccggttacgg caaggcatgg atctaccacc tgatcagcaa
     6001 aggtcgcttc ccgcagccgg tcaagattgg acttcgcgct attgccttcg ttgaaagtga
     6061 agtcgaggaa tggattcagt ctgttattga aacaaccaga aaaaatgttg cctgagcggg
     6121 agagggaaaa tgaatattga atgctttgta agtgagaatc atatgtgcaa ccttggtgag
     6181 aggttgttta acgagatttt tgatcttgat gccattgctg aactcgctga tgacaagttt
     6241 actagagaag ctgtttatat gctgagccag tatcttgttg gaaacacgag tggaccattt
     6301 aaagcaatca atatgcttgg agcccatgag caaattcata tttatgagat atggatttca
     6361 attattaaat cggctgatga gttaaggatt ttattcaagg caatacttcg acattgtatt
     6421 gaatgaaatc attagttgta aaacagataa aagt
//
LOCUS       DAPHDX010000196         6424 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPHDX010000196 DAPHDX010000000
VERSION     DAPHDX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.199"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(393..2063)
                     /gene="betA"
                     /locus_tag="RH058_004166"
     CDS             complement(393..2063)
                     /gene="betA"
                     /locus_tag="RH058_004166"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV3139669.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2077..3549)
                     /gene="betB"
                     /locus_tag="RH058_004167"
     CDS             complement(2077..3549)
                     /gene="betB"
                     /locus_tag="RH058_004167"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV3139670.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3563..4150)
                     /gene="betI"
                     /locus_tag="RH058_004168"
     CDS             complement(3563..4150)
                     /gene="betI"
                     /locus_tag="RH058_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV3139671.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4279..6312
                     /gene="betT"
                     /locus_tag="RH058_004169"
     CDS             4279..6312
                     /gene="betT"
                     /locus_tag="RH058_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV3139672.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALISLSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1318 a         1762 c         1768 g         1576 t
ORIGIN      
        1 atgattaatt aaagaagagt aaaaataaat cactgccaat aaattatacg aatcgtgttt
       61 aaagctccac gttgttttgg tctgacttat ttttgtcctc gtttcaagaa cccttttcgt
      121 atatgctaac cgtgagcggg agtgaacaag tcaccatagc caggaacact agcggcgtaa
      181 ttcttaagct gagtggcatt tcacctgcgg tttatcagtg cattcatagc accttcatca
      241 gccagattga acgagtccgc ttagaatttg ctggcgaggt tgtcactatg aagcatgaga
      301 gttaccccta tgttttatat aacgattctg tccccatacc tggaacggta actctcaatc
      361 agatcggttc ttgagtcagt tcaaatcacg actcattttt tcgctctcac cggcatccca
      421 tttgccacaa aatatcccgc cgtgctcctc ggcagcgctt cctgtccacg aatcatatcc
      481 gctattttct cgccaatcat aattgtcgtg gcgttcaaat tcccggtgat aatctgcggc
      541 ataatcgacg catccaccac acgcaggcct tctaacccgt gtacgcggcc ttcgccgtca
      601 accacggaca tctcgtcgta acccattttg caggtaccgc acggatggaa ggcggtttcg
      661 gcgtggttac gcacgaactc atcgagctgt tcatccgtct gacattccgt gccggggctg
      721 atttcgcggc cacgatactg atccagcgcg ggttgatgca tgatctcgcg ggtgatgcga
      781 attgcgtcgc ggaactcctg ccagtcctgc tcgtgcgaca tgtagttaaa cagaatcgcc
      841 ggatgctggt gcgggtcgcg ggatttaatc cgcacatgcc cacggcttgg cgagcgcatt
      901 gagccgacgt ggcactggaa accgtgctct ttcactgcat tcgagccgtt atagttaatc
      961 gctactggca ggaaatggta ctgaatattc ggccacgcaa attcctcacg gctgcgaata
     1021 aatccacctg cttcaaagtg gttgctggca ccaacgccag tgccgccaaa cagccactcc
     1081 gcaccgattt tcggttggtt ccaccactgc agggcagggt agagggaaac cggttctttg
     1141 cactcatatt gcagatacat ctccagatga tcctgaagat tttcgccgac gccgggtaat
     1201 tcatgcacca gcggaatatc aaactccgcc agcagttcag cgttgccgac gccggagcgt
     1261 tgcaggatct gcggtgaggc aatcgcgcct gcacataaca gcacttcttt gttggccgtt
     1321 gcgcgggttg ggatggtgct gtcgccttcc agccattcga cgcccaccgc gcgtttgccg
     1381 tcaaaaatga tgtgatcggt catagcgtga gtacgaatgg tcaggttagg gcgcgatttg
     1441 gcctgatcga gataaccgcg cgcggtactg gcgcgacggc cctgcggcgt gacggtgcga
     1501 tccatcgggc caaaaccttc ctgctgatag ccgttgagat cgtccgtgcg cgggtagcct
     1561 gcctgcacgc ccgcttcaat catcgcttca aacagcggat tgacgccggg tttggaggtg
     1621 gtgacgctca ccgggccatc gccaccgtgg tagtcgttct cgcccacatc gcgagtctcg
     1681 gccttgcggt agtagggcag gcagtcgaga taactccagt tctccagacc gggttcttgc
     1741 gcccagttat cgagatccag cgcattgcca cggatgtagc acatgccgtt gatcagcgac
     1801 gatccaccca ggcctttacc gcgtccgcac tccatgcggc ggttattcat aaacggttca
     1861 ggctctgttt cataggccca gttgtagcgt ttgccctgta gtgggaacgc cagcgctgcg
     1921 ggcatctggg tgcggaagtc aaagcgatag tccgggccgc ccgcttcaag cagcagcacg
     1981 gaggtattcg gatcttcagt cagacgggta gcgagaacgt tgccggctga gccggcacca
     2041 ataatgatgt agtcaaattg caaataaacc tcctggttag aatatggact ggaatttagc
     2101 catctcaacc tggatggact tcacctgggt gtaactctgg agcgtcatca cgccgttctc
     2161 gcgaccaatg ccggagtgtt tgtagccgcc aacgggcatc tctgccgggg attcgcccca
     2221 ggtgttgatc cagcaaatac ccgcttccag ctgatgaatg acgcgatgcg cgcggttcag
     2281 gtccgccgtc acgatgcccg ccgccaggcc gtagtcggta tcattggcgc ggcgaatcac
     2341 ttcgtcctca gattcataag tgagaagcga catcactggc ccgaagatct cttcacgcac
     2401 gatggtcata tcgtcgctgc aatcggtgaa cactgtcggt gcaacccatg cgccgttatc
     2461 gaagccatcg cctttcagta catcgccgcc gcacagtacg cgcgcgcctt cctctttgcc
     2521 tttggcgata tagcgcagca cgttatcgcg atgcgggaag ctgaccagcg ggccgaagtt
     2581 agtttgcgga tcgaaaacgt cgcccgcgcg aatgcgctca acgcgcgcca gaattttctg
     2641 ctcaaatgcg gctttgcatt tcgccggaac gaagacgcgg gtgccattgg tacacacctg
     2701 accggagctg aagaagtttg ccatcatggc gatatcggcg gcgagatcga gatccgcatc
     2761 atcgaaaacg atcagcggtg atttaccgcc cagttccatg gtcacttctt tcagggaaga
     2821 ggccgccgag ttagccatca cttttttgcc gctggcgaca ccgccggtaa atgacacttt
     2881 ggcaatgccc ggatgctcgg tcagatattg cccggtctcc gcgcccacgc ccggcaacac
     2941 gttaaatacg ccgtccggca ggcccgcttc gctgtaaatt tcagccagct ttaacgcggt
     3001 aagcggggta acttcgctcg gtttgaaaat cattgcgttg cctgccgcca gcgccggggc
     3061 ggatttccac agggcaatct ggatcgggta gttccatgcg ccaatccctg ccactacgcc
     3121 cagcggttcg cggcgggtat acacaaagga cgtttcacgc aacgggatct ggctgccttc
     3181 cagcgccggg atcagcccgg cgtagtactc cagcacgtcc gcaccggtaa cgatatcgac
     3241 ggttgaggtt tccgaatatg cttttccggt gtcgagggtt tccagttttg cgagttcgtc
     3301 attgcgttca cgcagaatat caacggcccg acgcagaata cgcgagcgct ccatagcggt
     3361 catcgccgcc cagatttttt gcccctgttg ggcactcttc acggcgcgat cgacatcctc
     3421 acgcccggcg gcctgcacgg tcgccagcac gttaccgttg gccgggttaa tggtctcgaa
     3481 ggtgcgaccg ctggtggcgg aggtataacc accatgtata taaagctgct gttctgccat
     3541 tcgggacatc acgtctcctc ggttaatcgg tgggtaaatg ctgagtaata aagtgacggg
     3601 tcagcgagtg tgcgagaggt ttatccagcg gtttgccgct cagtgccgcg cgcagccata
     3661 atccgtcaat cagcgcggca aggccgtagc ccgcttcctg tgcctgttgg cgcggtaact
     3721 cgcggcgaaa ttcgctcacc agattcgaca ataagcggcg gctactgacc tgctgtaaac
     3781 gatagagcat cggctgatgc atactgctgg cccagaacgc cagccaggct ttcatcgccg
     3841 cactgctcac ctgcgtttca tcgaagtttc cgccaacaat cgcctgtaat cgctgctctg
     3901 cactgccctg cggaagtgca tgtaatcgat tcaaaaccgc gtcacgcagc tgactggtga
     3961 tatcgcgcat ggttgcttcc agcagaccat ttttgtccct gaaatagtgg ctgatgatcc
     4021 ccgtagaaac gcctgcacgg cgggcgatct gcgcgatcgt tgcatcgtgc atgcccactt
     4081 catttattgc ttccagtgtg gcgtcgatca gttgtctgcg ccggatcgac tgcatcccca
     4141 atttgggcat tttcgccact ccattcatca gcgttgttta tctattaaag cggttattga
     4201 ttggacgttc aatataaaat gtgtcttaat tgttacgaat ttgattttaa atggtaacaa
     4261 taacagtggg gatactggat gacagacctt tcacacagca gggaaaagga caaaatcaat
     4321 ccggtggtgt tttacacctc cgccggactg attttgttgt tttccctgac aacgatcctg
     4381 tttcgcgact tctcggccct gtggattggc cgcacgctgg actgggtttc taaaaccttc
     4441 ggttggtact atctactggc ggcaacgctc tatattgtct ttgtggtctg tatcgcttgt
     4501 tcgcgttttg gttcggtgaa gctcgggcca gaacaatcca aaccggaatt cagcctgctg
     4561 agttgggcgg cgatgctgtt tgctgccggg atcggtatcg acctgatgtt cttctccgta
     4621 gccgaaccgg taacgcagta tatgcagccg ccggaaggcg cgggacagac gattgaggcc
     4681 gcgcgtcagg cgatggtctg gacgctgttt cactacggct taaccggctg gtcgatgtat
     4741 gcgctgatgg gcatggcgct cggatacttt agctatcgtt ataatttgcc gctcaccatc
     4801 cgctcggcgc tgtacccgat cttcggtaaa cggattaacg ggccgatagg gcactcagtg
     4861 gatattgcag cggtgatcgg caccatcttc ggtattgcca ctacgctcgg tatcggtgtg
     4921 gtgcagctta actatggctt gagcgtactg tttgatattc ccgattcgat ggcggcaaaa
     4981 gcggcactga tctccttgtc ggtgataatc gccacgatct ctgtcacctc cggtgtcgat
     5041 aaggggattc gtgtgttatc ggaacttaac gttgcgctgg cgctgggatt gatcctgttc
     5101 gtattgttta tgggcgacac ctcgttcctg cttaatgcgc tggtgctgaa tgttggcgac
     5161 tatgtgaatc gctttatggg catgacgctc aacagttttg ccttcgaccg tccagttgag
     5221 tggatgaata actggacgct cttcttctgg gcatggtggg tggcatggtc gccgtttgtc
     5281 ggcttgttcc tggcgcgtat ctcgcgtggg cgtaccattc gccagttcgt gctgggcacg
     5341 ttgattattc cgtttacctt cacgctgtta tggctctcgg tgttcggcaa tagcgcgctg
     5401 tatgaaatca tccacggcgg cgcggcattt gccgaggaag cgatggtcca tccggagcgc
     5461 ggcttctaca gcctgctggc gcagtatccg gcgtttacct ttagcgcctc cgtcgccacc
     5521 attactggcc tgctgtttta tgtgacctcg gcggactccg gtgcgctggt gctggggaat
     5581 ttcacctcgc agcttaaaga tatcaacagc gacgcccccg gctggctgcg cgtcttctgg
     5641 tcggtggcga ttggcctgct gacgctcggc atgctgatga ccaacgggat atccgcgctg
     5701 caaaacacca cggtgattat ggggctgccg ttcagctttg tgatcttctt cgtgatggcg
     5761 gggttgtata aatctctgaa ggtagaagat taccgccgtg aaagtgccaa ccgcgatacc
     5821 gcgccgcgac cgctggggct tcaggatcgc ctgagctgga aaaaacgtct ctcgcgcctg
     5881 atgaattatc cgggcacgcg ttacactaaa cagatgatgg agacggtctg ttacccggca
     5941 atggaagaag tggcgcagga gctgcggttg cgcggcgcgt acgtggagct aaaaagcctg
     6001 ccgccggaag agggacaaca gttggggcat ctggatttgt tggtgcatat gggcgaagaa
     6061 caaaactttg tctatcagat ttggccgcag caatattcgg tgccgggctt tacctaccgc
     6121 gcacgtagcg gtaaatcgac ctactaccgg ctggaaacct tcctgttaga aggcagccag
     6181 ggcaacgacc tgatggacta cagcaaagag caggtgatca ccgatattct tgaccagtac
     6241 gagcggcacc ttaactttat tcatctccat cgtgaagcgc cgggccatag cgtgatgttc
     6301 ccggacgcgt gattgttttt gcataaaacc gcgccatgtc tgcatatggc gcgggaatca
     6361 ttgactcgct gtaaaaaatt atgcatatat tatatatgat tgtcatgtca tatcctgcaa
     6421 ttcc
//
LOCUS       DAPHDX010000197         6409 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPHDX010000197 DAPHDX010000000
VERSION     DAPHDX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.200"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..374
                     /locus_tag="RH058_004170"
     CDS             <1..374
                     /locus_tag="RH058_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139673.1"
                     /translation="ENYIHPAGSIFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGM
                     GSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFAGDLFKYESVCI
                     AAMEHLKSKATAPIQNGPLPA"
     gene            complement(378..1769)
                     /gene="tcyP"
                     /locus_tag="RH058_004171"
     CDS             complement(378..1769)
                     /gene="tcyP"
                     /locus_tag="RH058_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV3139674.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1902..2492)
                     /locus_tag="RH058_004172"
     CDS             complement(1902..2492)
                     /locus_tag="RH058_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3139675.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2655..3323)
                     /gene="hxpB"
                     /locus_tag="RH058_004173"
     CDS             complement(2655..3323)
                     /gene="hxpB"
                     /locus_tag="RH058_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV3139676.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3470..4006
                     /locus_tag="RH058_004174"
     CDS             3470..4006
                     /locus_tag="RH058_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV3139677.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4047..4907)
                     /locus_tag="RH058_004175"
     CDS             complement(4047..4907)
                     /locus_tag="RH058_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV3139678.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5013..5303)
                     /gene="ghoS"
                     /locus_tag="RH058_004176"
     CDS             complement(5013..5303)
                     /gene="ghoS"
                     /locus_tag="RH058_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV3139679.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5404..6333)
                     /gene="pfkB"
                     /locus_tag="RH058_004177"
     CDS             complement(5404..6333)
                     /gene="pfkB"
                     /locus_tag="RH058_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV3139680.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1660 a         1598 c         1570 g         1581 t
ORIGIN      
        1 aagaaaatta tatacatcct gccgggagca tttttgctgc gattcaaaaa aatgagagtc
       61 tcctaaagtt tccttcgata atatcaatgc ttaatatcaa tcttcttttt aatccactaa
      121 atcttccggg aatgggaagt ggtgtgcttg aagatattat gagtattcca gatagttcac
      181 ttagaaaacg tctcgggtat gaggttttaa gcttctcgct acaggcacat tcattgagcc
      241 aggaatgtat tgataagttg gacatttttt tcgctggcga tctgtttaaa tatgagtcag
      301 tatgcatcgc cgcgatggaa catttaaaaa gtaaggcaac ggcccctata caaaacgggc
      361 cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct atccagaatg
      421 gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat cgagccacta
      481 acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa cagcgccacc
      541 agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca
      601 ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac
      661 atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgccag cattgccgga
      721 tacaaaccgg cgcagccgtt ctgaccaatg gttgcaccga aagaggcggc gaaactggcg
      781 atggattcag gaacgcccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca
      841 gcactggaac ggctggtaaa ggcaaacgtc agcacaggcc ataccttacg gaagtacttc
      901 agcggactca cgccattaat gcccagcaga atgccatgca ctgcaaacat aatcagcaga
      961 ccgaggtagg acgcgacaac gaaacttccc agtttgatga tgtcttgcaa gttagaacct
     1021 gcaaccactt tggtcattag agccagaacg ccgtaagggg tcaactgcat gacaaggcga
     1081 accagtttca tcacccagct ttgtagggta tcgatagcgg ctaagacgcg ttcacctttc
     1141 ggcgcatcat ccttcagcag tttcaatgca gctacgccga ggaatgcggc aaaaattacc
     1201 acgctgataa ttgacgtcgg attggctccg gtaagatcgg caaacgggtt tttcgggata
     1261 aaggacaaga ccagctgcgg aacgctcaga tcagagactt taccaacata gttactttca
     1321 atggcgttca gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca
     1381 aacaggttgg tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta
     1441 ccgatggtca gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg
     1501 ctcagaatag aggcgaagac taacggcata acgatcattt gcagcagttg aacatagccg
     1561 ttaccaacga tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa
     1621 atggtatgca gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc
     1681 gccagactcc actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc
     1741 acgatgttcg caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca
     1801 ctgcgtagca gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt
     1861 caaatggaat tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc
     1921 ttaataagcc gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca
     1981 gcgctgttct cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag
     2041 ataaaacgtt ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag
     2101 cggcatggcc agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc
     2161 aaataaccca gcaccattcc ttgtagtgca tcagccggaa tgaaccagcc ttccggaacc
     2221 ttaaggtaaa aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga
     2281 tgcccaaaag cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac
     2341 tttgggtgat cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac
     2401 cagtcaccct gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca
     2461 atagaaaaga gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt
     2521 acagtatagg atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa
     2581 ttgacgatta atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc
     2641 actgcccctg gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt
     2701 ttgacgtttg ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg
     2761 gaacgcatgc gggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg
     2821 caggtcagtg ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc
     2881 ttgctgtaag gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac
     2941 atggtcaaca ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa
     3001 ccttgttctt tgcataacgc aacagcttcg cgcacgcctg gtaataatgg acgtgtctct
     3061 tcaaccagtg aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca
     3121 ttccacggtt gccgggcgta ccaaagatcg accaccatat cgatgcgtaa acctaaggtg
     3181 tccggcagct cgttacgacg ggagatatcc acccccaggc ttgccatcac atccagttcg
     3241 gctcgatccc ataaaggttc tgagtcgata agtaatccat ccatatcaaa aattgcagca
     3301 agaatctgac gcggggttga cattacaacc tctcctttag agcattatgt gtaaggtatt
     3361 aagccgtgct tatgattaag cgcctatcat atcgccttta aagaatgggc gaaaacagat
     3421 atctacaggt aaactaagtg aaacgacgcg tcttaatcaa ggagaactca tgacgtatca
     3481 acaagctgga cgcattgctg ttttgaaaag gattttgggt tgggtgattt ttatacctgc
     3541 attgatctct acattgattt ctttgctgaa atttatgaat acccggcagg aaaaccagga
     3601 aggcattaat gcggtcatgc tcgactttac tcatgtcatg atcgatatga tgcaggcgaa
     3661 tacgcctttt ttgaatctgt tctggtataa ctccccgacc cctaatttta atggtggcgt
     3721 gaacgttatg ttctgggtga ttttcatcct gatttttgtc ggactggcgt tgcaggattc
     3781 cggtgcccgg atgagtcgcc aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat
     3841 tctggaaaaa gccaaagggg aagaggggct aacgcgtgaa caaattgagt cccgtattgt
     3901 tgttccgcac catactattt tcctgcagtt tttctcacta tatatcctgc cggttatttg
     3961 tattgctgca ggttatgtgt tcttttctct gcttgggttt atttaatcaa aaggccgcac
     4021 ggagcggcca ttatcctcaa cccatatcat gctgctaata atctatccaa tgactgctga
     4081 gcaataacca aatgctgacc gccaaataat cttgcacgat ttaacagcgt gtagagttgg
     4141 taaaccggtt gacgttcaag gaaatcggca ggtagcgggg atactgactg atagccgtca
     4201 tagatttgtg gcggctgttc agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca
     4261 ccccagtagc aggcagggtc gaaaatgtac gggccatccg gacccagcgc acagttgccg
     4321 gaccataaat cgccgtgcaa cagagaaggc tgcggttgat gcgaggccag acgctgctga
     4381 atatgctcga cgatggcgtc gatattgccg aaagcgatcc ctttctctgc tgccagttcc
     4441 aactgccagc caatccgttg ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg
     4501 ggctgtggag ttgtggagag cgagttatca aaatcgaggc caaattgtgg ttggtcactc
     4561 cattgatgta aacgcgcaat ttgctgacca agaataaatg cgctatgcgc atccagcgga
     4621 cgaggtggga gataatccat caccagaaaa ctgtagtcac ggtcagcgcc tactgcccaa
     4681 accttaggca cggtgacggt tttactacgc gacagtaact ccagttggtc ggcttcggcg
     4741 gtaaaaccgg gaagcagttc cctttcatca catttgacga aaaagtcatg tcctgcatag
     4801 cgcaaatgcc atgcggcatg gacttctccg ccaggcagtt cattacgcag ttcgatttcg
     4861 ccttcaccta actgctcgct caaaagacga ctgattgcct gccacatgat gtctctccca
     4921 tgttgtctgc cagatcataa ggttagcgca agaatgcggt gtaaaaacac gagctaacgc
     4981 acaccacaga ctgattattg cccaaaaacc atttattttc tgttgctatt ccattcctcc
     5041 caggtcgtga tggtgatttc aggatctttg ccggttgcac tttgggtaag ccccgaaacc
     5101 agttctctaa tttcttcttc actttgggta ctgataagtc caaaagtgtt agtccccagc
     5161 tcatggatat ttccctcatc atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa
     5221 ttattcaact cgttaatttc agtcaatgta tcctcatgca acatgacggt tatgacataa
     5281 cggacaagat cgccactggc catagttcac ccctttgtta tcacatgact tttgagcata
     5341 gtcggagaaa cgcgttgccg acaggttggt ggtgattccc ccaatgctgg gggaatgttt
     5401 ttgttagcgg gaaaggtaag cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt
     5461 tccctgattg agtgtggctg cactccccgc agctacgcca aaacggacca tctcttcaag
     5521 agaggcattt tctgccagtt tcagtgtcat cgcgccgacc atgctgtcac cagcgccaac
     5581 ggtactctgg cttttcaccg gtggtggcac cacctgaatg cagttttcac tatcaacacc
     5641 cagcgctcct tgtggaccaa gggaaacgac aacccgtttg gccttgccgc tattaacgat
     5701 ttcctgcgcg gctttgcgga catcgtctgg ctgggtgagt tcgcgattca ccagcgcact
     5761 gagttctttt tggttaggct taaccaactc gatgttacca atcgccagtg ctgcacttaa
     5821 cgcttcgcca gaactgtcga tgatgcagcg gatcccttgg ttttgcgcag cggaaatcag
     5881 ttgggttaat ttttccagct tcacacctgg cggcaggctt ccgcttatga ccaggatggc
     5941 cccggattca atttccagaa cttgctcttc aagctggcga aactcatctt catttaatgc
     6001 cgcgcctggc ataacaaaac gatactgctc accgcttgct tccacatgta cgtgtaaatt
     6061 ctgccgggtc cagtctttgg cttctacagt agcgacgggg acattttcat ccgccaacag
     6121 agaaaccagg tgttcgccgg tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc
     6181 aagatgggca atggcgcggg cgacgttgat gccgccgccc ccgggttcga acaccggtgc
     6241 ggtacagcgc agttttcctt cgggataaat ttgcggggta attgttgcgc tatcgagaga
     6301 gggcgcaagt gtcaacgtat agatacgtac catcatttcc tcctataggc tgatttcagt
     6361 ctggcaccga atcagccgaa agtgaagaaa ttaacaatat gatttatta
//
LOCUS       DAPHDX010000198         6370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPHDX010000198 DAPHDX010000000
VERSION     DAPHDX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.201"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..419)
                     /locus_tag="RH058_004178"
     CDS             complement(<1..419)
                     /locus_tag="RH058_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HDV3139681.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELID"
     gene            complement(564..1067)
                     /gene="cheW"
                     /locus_tag="RH058_004179"
     CDS             complement(564..1067)
                     /gene="cheW"
                     /locus_tag="RH058_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV3139682.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1088..3052)
                     /gene="cheA"
                     /locus_tag="RH058_004180"
     CDS             complement(1088..3052)
                     /gene="cheA"
                     /locus_tag="RH058_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV3139683.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3057..3983)
                     /gene="motB"
                     /locus_tag="RH058_004181"
     CDS             complement(3057..3983)
                     /gene="motB"
                     /locus_tag="RH058_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV3139684.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3980..4867)
                     /gene="motA"
                     /locus_tag="RH058_004182"
     CDS             complement(3980..4867)
                     /gene="motA"
                     /locus_tag="RH058_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3139685.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4994..5572)
                     /gene="flhC"
                     /locus_tag="RH058_004183"
     CDS             complement(4994..5572)
                     /gene="flhC"
                     /locus_tag="RH058_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV3139686.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5575..5925)
                     /gene="flhD"
                     /locus_tag="RH058_004184"
     CDS             complement(5575..5925)
                     /gene="flhD"
                     /locus_tag="RH058_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV3139687.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         1539 a         1678 c         1517 g         1636 t
ORIGIN      
        1 taatcaatca gttcagttaa cgctgtgtaa tagtttttat atttttcatc aatattacga
       61 ctggtagcga ccatttcagg taacggtgcc atgcttttga attttttata atgcgtcgct
      121 gcctgcgcca atgttttcct ggcgctatcg agcaattcaa ctttggcgtt actttgttga
      181 ttagaggaat ccatcatcat ccgcaccgct gaacgactca ggttaatgcg cgtttgcagc
      241 attaaatccc aggttgacgt cagctcgccc tgctgttccc gcaattgatt ggaaaccaca
      301 aagctcttct ggctatggtg aagggaagaa aaaaacagac tgccggaaat aagctgtaac
      361 agtgcgaata cccccagcac cattaccaac agcgtgacta cgcggatacg gttaatcata
      421 aggcaccttc ctgaaaacaa gttgatctcg ttatcggcaa gaagggggga aactttattg
      481 ctgatgccac ccgccgcgaa attgaaataa aaaacccgat gcgcagatca tcgggttcat
      541 ttcaattgag gaaatcggga gaattacgcc acttctgacg ccgcgctatc taacagcgcc
      601 atctcttcgc tgttcagcag tttttcgatg ttcaccagaa tcaacatacg gtcgcccagt
      661 gcgcccagtc cagtgagata ttctgttgaa agcgtcacgg caaactctgg tgccggacga
      721 atttgctccg ccgtcaatga aagcacgtct gaaacgccgt caaccacgat gccgaccacc
      781 cgctgtccga gattcaggac gataactacc gtgttgtcgt tatagtccac atccacctgg
      841 ctgaacttaa ttcgtaagtc aacaatcggc acaataacgc cgcgcagatt cgtgacgcct
      901 ttgataaacg ctggcgtgtt cgcaatccgt gttacctgat cgtagccacg gatctcctgc
      961 actttcagga tatcaatacc gtactcttca tcaccaaggg taaataccag aaattcctgg
     1021 cctgacggct cgctggccag ctttgttaca ttcgtcatac cggtcatatt gttacctttt
     1081 tactcattca ggcggcggtg ttcgccatac gttgttcgcg gtttatcgcc tgcaaggcgg
     1141 agacatcaac aatcagtgcc acgctgccgt cgccaagaat ggtcgcagca gaaatgccgg
     1201 ggactttgcg atagttactt tcaaggtttt taaccacaac ctggtgttga ccaattaatt
     1261 gatccaccag caaggcgtag cggcgaccgc cactttgtaa gatcacaaca attccctggg
     1321 tggcttcggt tttcgcgccc gcgacgttga acactttcca cagttcgacg atgggcagat
     1381 attcaccccg cacttccagc acccgctcgc cgccggccag tggatgcaga tcggcttcac
     1441 ggggttgcag tgattccatg acagcattca gcggcagaat gaaaacttca tccgcaacgc
     1501 gtacggacat gccgtcgagg atggccaacg tcagcggcag taaaatgcgg atcgtagtgc
     1561 cagtaccctg cttcgactgg atttcgacat gaccgcccat ctcctggata ttacgtttaa
     1621 cgacgtccat gccgacgccg cgcccggaga cgtcggtgac ctgctccgcc gtggagaagc
     1681 caggtgcaaa tatcagcatt gcaacttcgt cgtcgctcat gttttcgctg acagtcaaac
     1741 cttgcgaggc cgcttttgcc agaattcgct cacggtttag ccccgcccca tcgtcggtca
     1801 cttcaatgca aatgttgccg ccctgatgtt cggcagacag aattaaattt ccgacgctgt
     1861 ttttacctgc ggcgagccgt ttttctggca gttcaatacc gtgatcgagg ctattgcgta
     1921 ccaggtgggt cagcgggtcg ataatgcgtt ctatcaggct tttgtcgagt tcagtagaac
     1981 tgcccaccag cgtcagttct acctgcttgc cgagttttcc cgccagatca cgcaccagcc
     2041 ggggatagcg actaaaaaca tattccatcg gcatcatgcg aatcgacatc actgattcct
     2101 gcaaatcacg ggcgttacgt tgtaactgcc ccatgctggt tatcaaatca ccatgattaa
     2161 ccgggtccag ttcgctggaa cgctgggcaa gcatggactg ggtgataacc agctcgccga
     2221 cgaggttaat taattgatca accttttcta ccgctacacg gatgctggtg gattcattgc
     2281 tgcgcgtcgt tttttcccgc tccacgcggc cggttggcgc ttgttcggct gccagtttaa
     2341 gcactggtgg ggtggatatt tttggcgaga cttctactgt ttcaaaagta atctgatcgg
     2401 cttcaatcac aaaacagagt accgctgtga tgtcatcttc ggcaatgtcg cccggtaata
     2461 ttgccgagag cgaatccgcc cctttcacca cgtcagttaa cgttgtcaga tgtcccagtt
     2521 cttcttccag caggtcgact tccccggcct tcaggcgcga aaggataatt cgtcgcggcg
     2581 actgactgcg actctgctca tcttgcggtt cacttttggc aaccacactt aatcgggtca
     2641 ctgcggatgg cgtttcgcct ttcgcttcta atgccagttg acgcaaggcc tggcagatat
     2701 aatcgaagct ggcggcatcc ggctcttgcg actgtttata agcgtcgagc tgttcttgca
     2761 tgatgtcctt cgtttccaaa aacagattga taatgtcggt gttgagttgc atctcacctc
     2821 gtctggcttc atcgagcagg ttttccatca gatgcgtggt ttcctgcaaa acgctgaagc
     2881 caaaagttcc tgcccctcct ttgatcgagt gggcagcccg aaagatggca ttcaattgtt
     2941 cggcatctgg cgcttccggc tgcaaaacca gcagatgctg ctccatgtca gccaacagtt
     3001 cgtccgcttc atcaaaaaat gtctgataaa aatcgcttat atccatgctc acgctgtcac
     3061 ctcggttcgg ctgatggcat tgtgggaaca ctgacctgtg gtgcaacctc aggtttttcc
     3121 agggcgctta ctggctcatt ctggctttcg gcgttttcat gcaaaatggc ctgttcggct
     3181 tgtttgttca gtaccagcag gctgatgcga cggttgacgg catcatcagg tccgcgatcg
     3241 cttaagcgca tcgttgccgc catgccgaca acacgtaaca ctttgccgct atccaacccg
     3301 ccgaccatca gttcgcggcg ggatgcattg gcccgatcgg cagaaagctc ccagttgctg
     3361 taacctttct caccgctggc gtagggaaaa tcatcggtat gacctgaaag gctaatacgg
     3421 ttgggaatac cgttcagtac aggcgcgatg gcgcgcagaa tgtcgcgcat atagggttcg
     3481 acatcggcac tgccggttct aaacatcggg cgattctggc tatcgatgat ctgaatacgt
     3541 agaccttcct ggaccagatc gattttgaga tgtggacgca acgcccgcag tttcggatcg
     3601 gactctatca actggtcgag atcaccccgc aatttccgca atcgactttg ctccatgcgt
     3661 tttttcaact cttcgatgtt cggctgctta ttcacttccc cctggctttg ggtgtaatca
     3721 tcaccaccgc cgggaattgg gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca
     3781 gtcgccagtg gagtccggaa gtactccgca atctgaatca gctcttttgg gctggagatg
     3841 gagatcagcc acatcaccag aaaaaaggcc atcatcgcag tcataaagtc ggcataagca
     3901 atcttccacg atccatgtgc tgccccgtgg cttttggctt tgcgtcgttt gacgacaata
     3961 atcggatgcg cttgattctt catgcttcct cggttgtcgt ctgttgttgc ggatttttca
     4021 ccgcacgcac atgctcttcc agttcaataa acgacggacg ttcgctggaa tagagcgttt
     4081 tgcgaccaaa ctcaacggcg ataggcggtg cgtaaccgtt cagattagaa agcagagtga
     4141 ctttgacgca ctgcatcatt ttgctggttt cggcgctttt ctgacgtaaa acactcgcta
     4201 atggggaaat aaatccgtaa gccaataaaa tgccgaggaa agtccccacc atcgcatgtg
     4261 cgataagcgc acccagctcg gcggcaggac gatcggctga acctaacgcg tgaacgaccc
     4321 ccattacagc cgcgacaata ccaaacgccg gaagtgagtc cccgaccagc gccagactgt
     4381 tcgccgggac ttctgcctcg ctttcgtgcg tctcaatctc ttcatccatc agggcttcga
     4441 tttcgaaggt gttcatatga ccgctgataa tcagacgcag ataatcgacg ataaaatcaa
     4501 gcatgacgct atccgcgagg atgcgtgggt agctggcgaa gatctcgctc tcacggggat
     4561 tttcaatatc acgttccagc gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat
     4621 aaagcagagc cagcagatcc atatacattg ctttggtgta tttggagcga cgaaacagca
     4681 acggcagcgc cttcagcgtg cctttaatcg ctttgccatt attgccgacg ataaacgacc
     4741 caatccctgc accggcaata atcaccagtt cagcgggttg atagagtgct ccaaggcttc
     4801 caccggtcat caaataaccg ccgaaaactg taccgagaac aaccaggtaa cctaataaga
     4861 taagcacgac atcatccttc cactgttgac catgacagga tgttcagtcg tcaggcgtta
     4921 acgcgcgatt ggggcaaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc
     4981 ctcaccgtat cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat
     5041 cggcaggatt ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc
     5101 tgcaggcaaa gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt
     5161 tgcagctgga aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg
     5221 ttaatgccag cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg
     5281 ctttgatcac cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat
     5341 tacagaacat cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg
     5401 gcagcatgcc tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc
     5461 cgcgacttaa ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtgatcaatt
     5521 ccattgccag ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc
     5581 ccttttcttg cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag
     5641 catgatgccg gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt
     5701 aatcgtctgg tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt
     5761 aaccatttgc ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag
     5821 acgaaacata gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga
     5881 caagttgatg tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa
     5941 taaccaactt tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg
     6001 taaaaaataa ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt
     6061 ggtttaatat gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa
     6121 atcgacgcaa ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca
     6181 ataatgtgtg atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat
     6241 acttttgttt tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa
     6301 aatgaaacag gaataatcga aatgggatgt tgcgcacagt taaaataact caccgtaaat
     6361 aatcatctgc
//
LOCUS       DAPHDX010000199         6214 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPHDX010000199 DAPHDX010000000
VERSION     DAPHDX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.202"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            357..488
                     /locus_tag="RH058_004185"
     CDS             357..488
                     /locus_tag="RH058_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDV3139688.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            855..1283
                     /gene="tolA"
                     /locus_tag="RH058_004186"
     CDS             855..1283
                     /gene="tolA"
                     /locus_tag="RH058_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV3139689.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            1455..1829
                     /locus_tag="RH058_004187"
     CDS             1455..1829
                     /locus_tag="RH058_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HDV3139690.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            2081..2296
                     /gene="essD"
                     /locus_tag="RH058_004188"
     CDS             2081..2296
                     /gene="essD"
                     /locus_tag="RH058_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV3139691.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            2301..2645
                     /locus_tag="RH058_004189"
     CDS             2301..2645
                     /locus_tag="RH058_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDV3139692.1"
                     /translation="MTQKYELIVKGIRNFENKVTVTLALRDKERFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELSPEKRPIILEAQNVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(2611..2883)
                     /locus_tag="RH058_004190"
     CDS             complement(2611..2883)
                     /locus_tag="RH058_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139693.1"
                     /translation="MDNSTISLQELLDCISKLRDDVNALTVAFSYLAFSIPKEQMQPT
                     LASLQLESLNPKWSQQQQNSFKWLAILLEEKYAGEITISAESSENQ"
     gene            2989..3531
                     /locus_tag="RH058_004191"
     CDS             2989..3531
                     /locus_tag="RH058_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3139694.1"
                     /translation="MNVKIRYGLSAAVLALIAVGAPAPDILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNADDRKGACEAIRWWIKDGGRDCRLTKGQKNGC
                     YGQVERRDQESALACWGLEQ"
     gene            3528..3848
                     /locus_tag="RH058_004192"
                     /pseudo
     CDS             3528..3848
                     /locus_tag="RH058_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3850..5490
                     /locus_tag="RH058_004193"
                     /pseudo
     CDS             3850..5490
                     /locus_tag="RH058_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5490..6071
                     /locus_tag="RH058_004194"
     CDS             5490..6071
                     /locus_tag="RH058_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3139695.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEYQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
BASE COUNT         1695 a         1276 c         1594 g         1649 t
ORIGIN      
        1 ttttcagcat taaatattta tcattcatat ggtgtgggta gagtgaatat tgatgaggcg
       61 tcggggtgtt tcatccttag gcagcgtatt gatatagtca atgcagcacg agcaaaggcc
      121 ttcagccgtt tgacagtttt gttctgtgct cctgatcgtc tttcgggaag agacgttatt
      181 attctgaata gtgatgctat acagagggtt tgcgatgagt tcatggttgc taattcagaa
      241 ttatttgctc ttgttcagga gtacaacaga atagccagga cctgtggtat ggatgaactt
      301 cggattactc atctggggta gatacatatc tggattatca cctgttacgg taaaaagtga
      361 ttgcttactg tttttgtgaa tggcattgca gcagccggat aatgtcagtg ctggctgacg
      421 gtgtgctggt ggcgggtgtg gtagttgttg ctttcccgtt gctgaaaaag aaaacgccag
      481 actgttagcc gggtatcagt tagcgggaga aatttttaaa tacttcacaa ttcaggcggt
      541 tgactgttct ctggtttgcg gggagtttgt taaaagaaac tggcatggtg aatccccctg
      601 tgcggagggg caatcagcga gtaggtatat gggataatcg tggattcagg tgctggtact
      661 gaattcaccg ggaggccccc ggcaccatgc acttcaatag attctctcca cattatggat
      721 attctttcag aatatcccac gcagactttg tgtaaatgtt aacaaatgtg cgttttattt
      781 gatctgattc gctgtttgag cgtccaaaac aacggtatat ataatccttt actatatgac
      841 atatgtgagg aaaaatgggt tttcgtagcg catcaattct tacgttgatt attagtggga
      901 ttattatcgg gtgcactgat gctgtatcga caaattatca tgaccgtaca tcatattact
      961 ccgataaagc aatagagaca cagtatgtga gttcatctga acgtacttct gatgttagtg
     1021 aggatatccg tctgtatgcc catcaaatca agagcgccat cgaaaaacag ttcggggatg
     1081 cgagtaagta ttcaggaaaa gagtgtacac tgagaatgca tatggccccg aatggccttc
     1141 tactggaggt taaaagagaa agtggagacc tcgatttatg tcgtgaagcg atgaatgcga
     1201 taaagaatgc tgatatacct gccccccctt cgccggaagt atataaagta tttcaaaatg
     1261 gggtgctgga ttttaaaccc tgatatttat tgttttgtaa taaacggttt cggcttaggc
     1321 ttgttctgac acaactacgg cactgagcta aatctagcgg atagtcagct ctgagccagt
     1381 ggcggacgta acaactacta ttgctgagat tttaatggat tgaggagcaa gaagtgggat
     1441 taaagaaaat cgttatgttg actttttggg tcggttttgt tgcgggatgc acacctttac
     1501 acccttcaga ttgccacaaa actactgcta caggtagttg cagttcagga cgctgggatg
     1561 atcaggatga atggggggcg caagcgcggg gaatcagagc tgcaattaat gccaaacttg
     1621 atgagccgca taactggaaa gggaaaaaat gcaggttgca tatggaattc tctcaggatg
     1681 gcacggcgtt aaaaatatct accagtaacg gtgataaagc ctattgcgaa gcgataaagt
     1741 ccgcagctca taaagccaaa tttccggcct tcaacaatcc ggaagtctac agagattttc
     1801 agaaatctgg ctttgacatg cgaggttagc tcttcaatta ctatatctca ttcatagcaa
     1861 actgacagat ttgatgatgt tctatatacg aaacctgtga tgtcaagtct gagctaatac
     1921 aaataaacat aatatcagag aaatacattt tattagctcg ctacggcgag ctttttatat
     1981 tgcatcgtct ccagcatata tatcaattaa ggctccgatt gatgtgtctg gaagcctaca
     2041 cataataact atgccatccg ttccgtgcgg aggtgaggct atgaaatcca tggacaaaat
     2101 ttcaacaggc attgcctacg gcacctccgc aggcagtgct ggctactggt ttttacagct
     2161 gctcgataaa gtcacgccct cacagtgggc agcaataggt gtgctgggta gcctggtatt
     2221 tggcctgctg acgtacctga caaaccttta tttcaagatt aaagaagaca agcgtaaggc
     2281 tgcacgggga gagtaattca atgactcaaa aatatgaact gattgtgaaa gggatccgca
     2341 attttgagaa taaagttacg gtaactttag cgttacggga caaagaacgc tttgacggtg
     2401 aaatttttga cctggacatc tcgctggacc gtgttgaagg tgccgcgctg gagttttatg
     2461 aggcagcagc cagaaggagc atcagacagg tcttcctgga tgttgctgcc gggttatgtg
     2521 aaggggatga gctgtcgccg gaaaagcgcc ccataatttt agaggcgcag aatgtgtgga
     2581 taacctacaa aggaaagcta ccgggaagaa ttactggttc tctgaagact ccgccgaaat
     2641 ggtaatttca ccagcatatt tttcttccag taatatcgcc agccacttga aagaattttg
     2701 ttgttgctgg gaccatttgg ggttgagtga ttcaagctgg agcgatgcca gtgttggttg
     2761 catttgttcc ttgggaattg agaatgccag atatgaaaat gcgacagtaa gggcatttac
     2821 atcatcccga agcttggaaa tgcagtcgag caactcctgt agagaaatgg tgctattgtc
     2881 cataaacaat cctctctatt gtatttaact attccttgcc tgattcaaca ggccgggaca
     2941 gataaacata tccagggttc agaaaccgat aaatcctgat aaaaatccat gaacgtaaaa
     3001 atcagatacg gcctgtcggc tgccgttctg gcactgattg ccgtcggtgc gcctgcgcct
     3061 gatattctcg accagtttct gaatgaaaaa gaaggtaacc acacaacggc ataccgcgat
     3121 ggttccggca tctggaccat ctgtcggggt gccacggtgg tggatggaaa acccgttttt
     3181 cccggtatga aactgtcgaa ggaaaaatgc gaccaggtta acgccattga acgggataag
     3241 gcgctggcat gggtggagcg caatattaaa gtgccactga ccgaaccaca gaaagcgggt
     3301 atagcgtcat tttgtcccta taacattggc cccggtaagt gtttcccgtc gacgttttat
     3361 aagcggctga atgccgatga tcgtaagggg gcatgcgagg cgattcgctg gtggataaaa
     3421 gatggtgggc gtgattgccg cctgacaaaa gggcagaaga atggctgtta tgggcaggtc
     3481 gagcggcgcg atcaggaaag cgcactggcg tgctgggggc tggagcaatg aaaattaatc
     3541 cggatcttat aggcgttgtc gttattgctg gcctttcggt cgctctcgtc aagagttgct
     3601 cttacgccag taatcttcag agcgataacg acgttctgcg aagtgacaac tctatgctgg
     3661 ggcaggtaat cgccacccag gcattcaact tcaatcgatt caatcaggtt gcagaacatg
     3721 ccaacagtct taactccctg atcgacacca gcaccgaaaa aaccgtaatc gaataccggg
     3781 agattctccg ccgtgaaaaa acctgtgatc tgcctgttcc tgctgatatc gctggtgggt
     3841 tgctcgaata acggatttgt tatgcctggt ggctggactg acagggggcg ttatgcttat
     3901 ggaatgttct ggcaatatca aaacaatgaa cgagccatcc actcaataat gatgagtaat
     3961 aagggcgatg atttgcgctc tgtgttctat gttgatggcg ctgctttccc tgtttttgcg
     4021 tttatcgaag atggcctgtc aatatccgca cctggtgctg atctcgttgt taatgatacg
     4081 acctataagt ttggggcaac aaatccggcg actgaatgta tcgcggcgga cgttatcctt
     4141 gattttaaga gtgggcgtgg tttttatgag tctcattcat taatcgttaa cgataacttg
     4201 tcgtgcaaaa aactttttgc cacagacgaa attgtagcgc gtggtggtaa tcagattcga
     4261 atgataggtg gggagtatgg ggcattatgg cgtaatgatg gcgctaaaac ttacctgctg
     4321 cttaccaatc aaggtgatgt ttatggtggc tggaatacat taagaccgtt tgctattgat
     4381 aacgcaaccg gcgaactggt tattggaacc aaactgtccg caagtctgaa cggtaatgca
     4441 ttaacagcaa caaagctgca aacgccaaga cgggtttctg gtgttgagtt tgatggttcc
     4501 aaagatatta ctttaaccgc cgcgcatgtg gctgcttttg ccagaagggc aacggataca
     4561 tatgccgatg cggatggtgg cgttccctgg aatgccgaat caggcgctta taatgtcacc
     4621 cgctctggcg acagctatat tctggttaac ttctataccg gagtcggaag ttgccggacc
     4681 ttgcagatga aggcgcatta cagaaatggt ggtctgttct accgttcttc aagagacggt
     4741 tatggttttg aggaagactg ggcagaagtt tatacctcga aaaatcttcc accagaaagc
     4801 tacccagtcg gcgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg
     4861 atgcaggggc agacttttga caaatctgcc tacccgaaac ttgcagccgc ttatccgtca
     4921 ggcgtgatcc ctgatatgcg tggctggacg attaagggca agcccgccag tggtcgtgcc
     4981 gtattgtctc aggaacagga cggcataaaa tcgcataccc acagcgccag cgcatccagt
     5041 actgatttgg ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac
     5101 actggtgcgc atacctatag tttaagtggc agcacgaatg cagctggtaa tcacagccat
     5161 agagatggcc gtcgatttaa ccccagtgtt tttaaagata cttatcaata tggttataca
     5221 agctcaggtc aaaatacctg gggtgtacaa ggctcagtag gtatgtctac gggggcgtta
     5281 gctaatacca gtacagatgg taaccatagc cactcactgt ccggcacagc agcatctgca
     5341 ggtgcacacg cacatactgt cggtattggt gcacacacgc actccgttgc gattggttca
     5401 catggacaca ccatcaccgt taacgctgct ggtaacgcgg aaaacaccgt caaaaacatc
     5461 gcatttaact atattgtgag gcttgcataa tggcattcag aatgagtgaa caaccacgga
     5521 ccataaaaat ttataatctg ctggccggaa ctaatgaatt tattggtgaa ggtgacgcat
     5581 atattccgcc tcatacaggt ctgccagcaa acagtaccga tattgcaccg ccagatattc
     5641 cggctggctt tgtggctgtt ttcaacagtg atgaggcatc gtggcatctc gttgaagatc
     5701 atcggggaaa aaccgtctat gacgtggctt ccggcgacgc gttatttatt tctgaacttg
     5761 gctcgttacc ggaaaatgtc acctggttat cgccggaagg ggaatatcag aagtggaacg
     5821 gcacagcctg ggtgaaggat acggaagcag aaaaactgtt ccggatccgg gaggcggaag
     5881 aaacaaaaaa caacctgatg caggtagcca gtgagcatat tgcgccgctt caggatgctg
     5941 cagatctgga aattgcaacg gaggaagaaa tctcgttgct ggaagcctgg aagaagtatc
     6001 gggtgttgct gaaccgtgtt aatacaacaa ctgcaccgga tattgaatgg ccagtagcac
     6061 ctatagggta aattcgtaat gattacctaa atacgttatt cttttgttca aaaagtgatt
     6121 tttctatagg tagggagtat gttcttggat taagaataaa aaatcgccat gaaagagctt
     6181 catggcgagt acataaacgt acaaaatatg atga
//
LOCUS       DAPHDX010000200         6058 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPHDX010000200 DAPHDX010000000
VERSION     DAPHDX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.203"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            215..1318
                     /gene="ompC"
                     /locus_tag="RH058_004195"
     CDS             215..1318
                     /gene="ompC"
                     /locus_tag="RH058_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3139696.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1430..2485
                     /gene="apbE"
                     /locus_tag="RH058_004196"
     CDS             1430..2485
                     /gene="apbE"
                     /locus_tag="RH058_004196"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV3139697.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2559..3623
                     /gene="ada"
                     /locus_tag="RH058_004197"
     CDS             2559..3623
                     /gene="ada"
                     /locus_tag="RH058_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV3139698.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADESLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3623..4273
                     /gene="alkB"
                     /locus_tag="RH058_004198"
     CDS             3623..4273
                     /gene="alkB"
                     /locus_tag="RH058_004198"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV3139699.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4349..5992
                     /gene="yojI"
                     /locus_tag="RH058_004199"
     CDS             4349..5992
                     /gene="yojI"
                     /locus_tag="RH058_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV3139700.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1435 a         1536 c         1640 g         1447 t
ORIGIN      
        1 gccattccgc aataatctta aaaagttccc ttgcatttac attttgaaac atctatagcg
       61 ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt ctgcattttt
      121 ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt ggcataaaaa
      181 agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac tgtccctcct
      241 ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca acaaagacgg
      301 caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg acaacaaaga
      361 tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc aggttactga
      421 ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg ctgaaaacga
      481 aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg tgggttcttt
      541 cgactacggt cgtaactacg gcgttgttta cgacgtaact tcctggaccg acgtactgcc
      601 agaattcggt ggcgacacct acggttctga caacttcatg cagcagcgtg gtaacggctt
      661 tgcgacctac cgtaacactg acttcttcgg tctggttgac ggcctgaact ttgctgttca
      721 gtaccagggc aaaaacggta gcgtaagcgg cgaaggcatg accaacaatg gtcgcgatgc
      781 tctgcgtcag aacggcgacg gcgttggcgg ttctatcact tatgattacg aaggcttcgg
      841 tatcggtgct gcagtttcca gctccaaacg tactgatgct cagaacaccg ctgcttacat
      901 cggtaacggc gaccgtgctg aaacctacac cggtggtctg aaatacgacg ctaacaacat
      961 ctacctggct gctcagtaca cccagaccta taacgcaact cgcgtaggtt ccctgggttg
     1021 ggcgaacaaa gcacagaact ttgaagctgt tgctcagtac cagttcgact tcggtctgcg
     1081 tccgtctgta gcatacctgc agtctaaagg taaaaacctg ggtaccatcg ctggtcgtaa
     1141 ctacgacgac gaagatatcc tgaaatatgt tgatgttggc gcgacctact acttcaacaa
     1201 aaacatgtcc acctacgttg actacaaaat caacctgctg gacgacaacc agttcactcg
     1261 tgacgctggc atcaacactg ataacatcgt agctctgggt ctggtttacc agttctaatc
     1321 tcgattgata tcgaacaaag ggcctgcggg cccttttttc attgttttca gcgtacaaac
     1381 tcattttttt ggtgtactct tgcgaccgtt cgcatgagga taatcacgta tggaaataag
     1441 ctttacccgc gtggcactgc tggctgccgc gctcttcttt gttggttgcg atcaaaaacc
     1501 acaacccgcc aaaacccacg ctactgaagt taccgttctt gaaggcaaaa ccatgggtac
     1561 cttctggcgt gccagcatcc cgggcattga tgccaaacgc agtgccgaac ttaaagaaaa
     1621 gattcagacc cagctggacg ccgacgatca gctgctttcg acctataaaa aagattccgc
     1681 gctgatgcgc tttaacgact cgcaaagttt gtcgccgtgg ccagtaagtg aagcgatggc
     1741 cgaaatcgtc actacctcgc tgcgcattgg cgcgaagacc gatggtgcga tggatattac
     1801 cgtcgggccg ctggtgaatc tgtggggctt tggcccggaa caacagccgg ttcaaattcc
     1861 gagccaggaa cagatcgatg cgatgaaagc caaaaccggc ttacagcacc tgacggtcat
     1921 caatcagtcg catcagcaat atctgcaaaa agacctgccg gatttatata tcgatctctc
     1981 caccgtcggt gaaggttatg cggcggatca cctggcacgc ttgatggagc aggaagggat
     2041 ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc cgtggtatga acggtgaagg
     2101 ccagccgtgg cgggtagcga ttcaaaaacc aaccgataaa gaaaacgcgg ttcaggctgt
     2161 ggtggatatc aacggtcatg ggattagcac ctctggcagc taccgtaact attacgaact
     2221 ggacggcaaa cgtctttccc atgttatcga tccgcaaacc gggcgtccca tcgaacacaa
     2281 tctggtatcc gtgacggtga ttgctccgac ggcgctggaa gccgatgcct gggatactgg
     2341 cttgatggta ctcgggccgg agaaagccaa agaggttgtt cgccgggaag ggctggcggt
     2401 ctatatgatc accaaagaag gcgatagctt taaaacctgg atgtcgccgc agtttaaaag
     2461 cttccttatc agcgaaaaaa attaaagcgc aagattgttg gtttttgcgt gatggtgacc
     2521 gggcagacta aaggctatcc ttaaccaggg agctgattat gaaaaaagcc acatgcttaa
     2581 ctgacgatca acgctggcaa tctgtcttag cccgcgaccc gaatgccgac ggcgaattcg
     2641 ttttcgccgt gcgtaccaca ggcatctttt gccgtccgtc ttgccgcgcc agacatgctt
     2701 tgcgggaaaa cgtctccttc tacgcaaatg ccagcgaggc actcgccgct ggctttcgcc
     2761 cctgcaaacg ttgtcagcca gacaaagcca atgcccagca acatcggttg gataaaatca
     2821 cccacgcctg tcgactgctg gaacaggaaa cgcctgtaac gctggaagcc ttagccgacc
     2881 atgtggcgat gagtccgttc catctgcatc ggttgtttaa agcgactacc ggaatgacgc
     2941 ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg
     3001 agagcgtgac gacgtctatt cttaacgccg gattccccga cagcagcagt tactatcgca
     3061 aagctgacga atcgctgggc atgacggcta aacaattccg tcacggtggc gaaaatctgg
     3121 cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg cctggtggca gaaagcgagc
     3181 gggggatttg cgcgatattg ctgggcgatg atgacgcgac gctaatcagc gagttgcagc
     3241 agatgtttcc cgctgccgac aacgcgcctg ccgatctgac gtttcagcaa catgtgcgtg
     3301 aagtgatcgc cagcctcaat caacgcgata cgccgctgac gttaccgctg gacattcgcg
     3361 gcactgcttt tcagcaacaa gtctggcagg cactgcgcac gataccttgc ggtgaaaccg
     3421 tcagttatca gcaactggct aacgccatcg gcaaaccgaa agcggtacgg gccgttgcca
     3481 gcgcctgtgc cgccaacaag ctggctatcg taataccctg tcatcgggtg gtccgtggtg
     3541 atggcacact ttccggttac cgctggggcg tgtcgcgtaa agcgcaactg ctgcgccgcg
     3601 aagctgaaaa tgaggagagg taatgttgga tctgtttgcc gatgctgaac cgtggcaaga
     3661 gccactggcg gctggtgcgg taattttacg gcgttttgct tttaacgctg cggagcaact
     3721 gatccgcgat attaatgacg ttgccagcca gtcgccgttt cgccagatgg tcacccccgg
     3781 gggatatacc atgtcggtgg cgatgaccaa ctgtgggcat ctgggctgga cgagccatcg
     3841 gcaaggttat ctctattcgc ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc
     3901 acagagtttt cataatttat gtcaacgtgc ggctacggcg gcgggctatc cagatttcca
     3961 gccagatgct tgtcttatca accgctacgc tcctggcgcg aaactgtcgc tgcatcagga
     4021 taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt tctctgggct tacccgcgat
     4081 ttttcaattt ggtggcctga aacgtaatga tccgctcaaa cgtttgttgt tggaacatgg
     4141 cgatgtggtg gtatggggcg gtgaatcgcg gctgttttat cacggtattc aaccgttgaa
     4201 agcggggttt catccactca ccaccgactg ccgctacaac ctgacattcc gtcaggcagg
     4261 taaaaaagaa taaaaataag aattattatt gctgtgcgcg cgaagatgtt taaactgcgg
     4321 gctgtaattc attgtccggg ttttctgcat ggaacttctt gtacttgtct ggcggcagta
     4381 tcgctggcca tttatcagtg tgatggcgct aagcctcgcc agtgcggcat taggcattgg
     4441 cttaattgct tttatcaatc agcgccttat cgaaacggcg gataccagtc tgctggtatt
     4501 gccggagttt ctgggattat tgctgctgtt gatggcagtc actctcggat cgcaactggc
     4561 gctcaccact ttggggcatc acttcgttta ccgactgcgt agtgaattta tcaagcggat
     4621 tctggatacc cacgtcgagc gcattgaaca actcggtagc gcctcgttgc tggcgggatt
     4681 aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt cttccggaac tggtgcaggg
     4741 gatcattctc actatcggtt ccgcggcgta tctgtggatg ctgtcgggca aaatgttgct
     4801 ggtaacggct atctggatgg cgatcaccat ctggggtggt tttgtactgg tggcgcgggt
     4861 gtacaaacat atggcgaccc tgcgtgaaac cgaagacaag ctgtacacgg attttcaaac
     4921 cgtactcgaa gggcgcaaag agctgactct gaaccgggaa cgcgccgagt atgtgtttaa
     4981 caacctctac atccccgatg cgcaagagta tcgccatcat attattcgcg cagacacctt
     5041 ccatcttagc gccgtgaact ggtcaaacat catgatgctg ggcgctatcg gcctggtgtt
     5101 ctggatggcg aacagtctcg gttgggctga taccaacgtt gctgcgacct attcgttgac
     5161 gctgttattc ctgcgtacgc cgctgctttc ggcggttggc gcattgccga cgctgctgac
     5221 ggcgcaggtg gcgtttaaca agctgaacaa attcgcgctc gcgcctttca aagctgagtt
     5281 tccgcgcccg caggcttttc ccaactggca aacgctggag ctgcgtaacg tgacgtttag
     5341 ctatcaggat aacgcgtttt ccgttgggcc gattaacctc accattaaac gtggcgagct
     5401 gctgtttctg attggtggca acggtagtgg aaaatcgacg ctggcgatgt tgctgacggg
     5461 cttgtaccag ccacaaagcg gcgaaatctt gctggatggc aaacctgtca gtggcgaaca
     5521 accggaagat tatcgcaaac tgttttcggc agtgtttacc gatgtctggc tgtttgatca
     5581 actgctgggg ccggagggta aacccgctaa cccgcaactg gttgagaagt ggctggcgca
     5641 gctgaaaatg gctcataagc ttgagttaag caacgggcgt attgttaacc tgaagttatc
     5701 aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg gcagaagaac gcgatattat
     5761 cctgctggat gaatgggcgg cggatcagga tccacacttc cgtcgtgagt tttatcaggt
     5821 gttgctgccg ctgatgcagg agatgggtaa aactattttc gctatcagtc atgatgatca
     5881 ttactttatc cacgccgacc gcctgctgga aatgcgcaat gggcaactta gcgagctgac
     5941 gggcgaagag cgcgatgccg cttcgcgtga tgccgttgcc cggacggcat aatattacga
     6001 cgctgacctg gcggactgct gccgtcaggt caatatgcta aaaatccctt catttcta
//
LOCUS       DAPHDX010000201         5992 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPHDX010000201 DAPHDX010000000
VERSION     DAPHDX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.204"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..359)
                     /gene="quuD"
                     /locus_tag="RH058_004200"
                     /pseudo
     CDS             complement(1..359)
                     /gene="quuD"
                     /locus_tag="RH058_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
     gene            complement(377..472)
                     /locus_tag="RH058_004201"
                     /pseudo
     CDS             complement(377..472)
                     /locus_tag="RH058_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            467..1090
                     /locus_tag="RH058_004202"
                     /pseudo
     CDS             467..1090
                     /locus_tag="RH058_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            1218..1754
                     /gene="yfdR"
                     /locus_tag="RH058_004203"
     CDS             1218..1754
                     /gene="yfdR"
                     /locus_tag="RH058_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV3139701.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            1745..2107
                     /locus_tag="RH058_004204"
     CDS             1745..2107
                     /locus_tag="RH058_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139702.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            2107..2412
                     /locus_tag="RH058_004205"
     CDS             2107..2412
                     /locus_tag="RH058_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139703.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPVE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMTQQEVK"
     gene            2639..3802
                     /locus_tag="RH058_004206"
     CDS             2639..3802
                     /locus_tag="RH058_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3139704.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(3818..3894)
                     /locus_tag="RH058_004207"
     tRNA            complement(3818..3894)
                     /locus_tag="RH058_004207"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3858..3860),aa:Arg,seq:tct)
     gene            4141..4773
                     /gene="fimZ"
                     /locus_tag="RH058_004208"
     CDS             4141..4773
                     /gene="fimZ"
                     /locus_tag="RH058_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV3139705.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(4776..5291)
                     /gene="sfmF"
                     /locus_tag="RH058_004209"
     CDS             complement(4776..5291)
                     /gene="sfmF"
                     /locus_tag="RH058_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV3139706.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(5302..>5992)
                     /gene="fimH"
                     /locus_tag="RH058_004210"
     CDS             complement(5302..>5992)
                     /gene="fimH"
                     /locus_tag="RH058_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV3139707.1"
                     /translation="AMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRL
                     KVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVE
                     FDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMV
                     SDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRARPISITGQKPTEGPF
                     SALGYLRVDYQ"
BASE COUNT         1761 a         1392 c         1380 g         1459 t
ORIGIN      
        1 tccatctcta accggatatc taatgccatt aacatccctt caattatgcc ctcagccttc
       61 tgtaaccttt tcccgatata accatcagat cagcaatgct tacctgccag tgacatgaat
      121 gtcataccga ctacataata atctactaat aaatcgtgca aatcgctgtt gttctttttc
      181 agacgggcca tgcacccgca aatgatcatc gcgtcatcgt cacaacattg cgggcgagat
      241 tttacttttg aaggaattaa tcccttaaaa ccggcggcaa tggacgacca ggtcacatct
      301 tcatgattat tagccgccca cgctccccaa cgttcaagaa ccatctgaat atcacgcatt
      361 aactttctcc acaaaatcag gccagcacac caatcgccag tgcgcgatcg ataaaacgaa
      421 atatcagctc cagctgggag ccatacttct cttcaaatgc cacggtaccc gttattctaa
      481 agatcttgca gatgaaggca cccgctgctt tatcgatgcc gataatatgc gtgccgtcag
      541 tgtgcttaac ctgggtacta ttgatgagcc aggtcacgca gataacactg ccactctcaa
      601 actgaaaaag acagcaccgt tctctgctct gttgtctgtt aacggcgagc gtaactccca
      661 gaagtcactg gcagaatgga tcgaagactg ggccgactac cttgtgggct ttgatgctaa
      721 tggtgacgcc attcaggcaa caaaagcggc tgcggcagtc cgtaaaatca cgattgaagc
      781 aaaccagacc gctgattttg aagataatga cttcagcggc aaacgctccc tgatggagtc
      841 tgtcgaagcg aagaccaaag acattatgcc agtggcattt gaatttaaat gcgttccgtt
      901 tgaaggtctg aaagaacgtc cgtttaaatt acgcctcagc attatcactg gcgatcgtcc
      961 tgtactggtt ctgcgcatta ttcagctgga agcggtgcag gaagaaatgg ctaacgaatt
     1021 tcgtgatctg cttgttgaga aattcaaaga cagcaaagta gaaaccttta ttggtacttt
     1081 caccgcctga tttcattact gcaaatgccc ctgcgggggc atttatggaa acgtaattaa
     1141 ctcaataatc gccggatggt gagggcttcc ttttaccaga attcagcgcg gtgcagtgca
     1201 tatacgtgga gaacaaaatg tcatttatta aaactttttc cgggaagcat ttttattatg
     1261 acaagataaa taaagacgac atcgatatta acgatatcgc ggtttccctt tcaaatatct
     1321 gtcgctttgc cggtcatctt tcgcacttct acagcgtcgc ccaacatgcg gttctttgca
     1381 gccagctggt gccgcaggaa tttgcttttg aagcgttaat gcatgatgca acagaagcgt
     1441 attgccagga cattcccgca ccactgaaac gccttcttcc tgactataaa cggatggaag
     1501 aaaaaataga cgccgtaatc cgtgagaaat acgggttacc cccagttatg agtacacccg
     1561 tgaaatatgc cgatctcatc atgctggcaa ccgaacgccg cgatctcggg cttgatgatg
     1621 gctctttctg gcctgtactg gaaggcatcc cggcaacaga gatgttcaac gtgattccac
     1681 tggcaccggg tcatgcctac gggatgttta tggaacgttt taacgattta tcggagttac
     1741 gcaaatgcgc atgaatgttt tcgaaatgga agggtttctt cgcgggaaat gtgtaccgcg
     1801 agatctgaaa gtgaacgaaa caaatgctga gtacctggta cgtaaattcg acgcgcttga
     1861 agctaaatgt gcggcactgg aaaacaaaat aataccagtg tcagctgaac tgccaccagc
     1921 aaatgaaagt gttctgttat ttgatgctaa tggagaaggc tggctgattg gctggcgttc
     1981 tctctggtac acctggggac aaaaagaaac cggagaatgg cagtggacat ttcaggtcgg
     2041 ggaccttgaa aacgtcaata tcactcactg ggcagtaatg ccaaaagcac cggaggctgg
     2101 agcataatga ccacatttac caataaagaa ctgattaaag aaatcaaaga acgaatcagc
     2161 agcctagagg ttcgagacga tattgagcgc cgtgcttatg aaatcgcact cgtatctctg
     2221 gaagtagagc cagttgaacg cgaagcctat gaattattca tggaaaagcg tttcggtgac
     2281 ttagtagatc gtcggagagc aaaaaacggc gataacgaat acatggcatg ggatatgact
     2341 ctcggttgga tcgtctggca gcaacgagct ggtatccatt tttcaacaat gacacagcaa
     2401 gaggtgaaat aatggagcca tacagcctca cactcgatga ggcctgtcag tttcttaaga
     2461 tatccagacc aaccgccacc aactggatac gaacaggccg cctacaggca acacgtaaag
     2521 atccaaccaa gccaaaatct ccttacctca caacacggca agcttgcatt gcggcgcttc
     2581 agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag gaaagaaaat
     2641 gtcactcttc cgcagaaatg aaatatggta tgcctcgtat tcgctcccgg gcgggaaacg
     2701 aattaaggaa tctcttggca caaaggacaa gcggcaagct caggagttgc acgacaagcg
     2761 aaaagcagaa ctctggcgag tagaaaagct aggggattta cctgatgtca cttttgaaga
     2821 ggcctgccta agatggcttg aggaaaaagc tgataaaaaa tctctcgatt cagataaaag
     2881 ccggattgag ttctggcttg aacattttga gggtataagg cttaaagata tctcggaggc
     2941 aaagatttac tctgctgtaa gcagaatgca taacagaaag acgaaagaaa tatggaaaca
     3001 gaaagttcag gccgccatca ggaaaggtaa agaaccgcct gtttatgaac caaagccagt
     3061 atcaactcag acaaaggcaa agcatcttgc catgataaag gccattctcc gtgctgcaga
     3121 acgcgactgg aagtggctgg aaaaagcgcc tgtcatcaag ataccagcgg tcagaaacaa
     3181 gcgagtcaga tggctggaaa aggaggaagc aaaacgcctt attgatgagt gccccgaacc
     3241 actgaaatct gtcgtcaagt ttgcgctggc aactggtctg agaaagtcga acatcataaa
     3301 tctggaatgg caacaaatcg acatgcagcg acgagttgcc tgggtgaatc cagaagagag
     3361 caaatcaaac cgcgccattg gtgtggcgct gaacgatacc gcctgtaaag tgttgcgtga
     3421 tcaaataggc aagcatcaca aatgggtgtt tgtacatacc aaggcggcta agcgagcaga
     3481 tggaacatca acgcctgcgg tcaggaagat gcgcatcgac agcaagacat catggctatc
     3541 agcttgtcgt cgtgcaggaa ttgaagattt ccgtttccat gacctcagac acacctgggc
     3601 aagctggctg atccagtcag gcgtcccatt atctgtgctt caggaaatgg gcggatggga
     3661 gtccatagaa atggttcgta ggtatgctca ccttgcgcct aatcatttga cagagcatgc
     3721 aaggaaaata gacgacattt ttggtgataa tgtcccaaat atgtcccact gtggaattat
     3781 ggaggatata aagaaggcgt aactgattga attgtaatgg cgcgccctgc aggattcgaa
     3841 cctgcggccc acgacttaga aggtcgttgc tctatccaac tgagctaagg gcgcgttgat
     3901 accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc cttgctgagt caatggcttt
     3961 tgatctggtt gctgaacaag tgaacgaccg cgtctgattt tctgatttat ttcgctatag
     4021 cggcaaacaa acacacacac accgctgcgc gtctgaatca agaaaacccg tattttcatg
     4081 tatcaaagta caatttaccg ccctaacgga aaattgtccg ctcctatgag actggtaact
     4141 atgaaaccaa cgtcggtgat cattatggat actcatccta tcatcagaat gtctattgaa
     4201 gttctgttgc aaaaaaacag tgaattgcag attgtcctga aaacggatga ttaccgcata
     4261 accatcgatt atctccgaac ccgtcctgtt gatttaatca ttatggatat agacttgccc
     4321 ggaacagacg gttttacctt cctgaaaagg atcaaacaaa tccagagcac agtgaaagtg
     4381 ttatttttat catcgaaatc agaatgcttt tatgctggca gagcgataca agctggtgct
     4441 aacggttttg tcagtaaatg caatgatcag aatgatattt ttcatgccgt tcagatgatc
     4501 ctctccggat acacgttttt tcccagcgaa acgcttaact atataaaaag caataaatgt
     4561 agtacgaata gttcaacggt cactgtgcta tctaatcgtg aagtgaccat attacgttat
     4621 ctggttagcg gattatctaa taaagaaatt gccgataagt tattacttag caataaaaca
     4681 gttagtgcgc ataaatctaa tatttatggc aagctaggtt tgcattcaat tgtagagctt
     4741 atcgactacg ccaaattata cgaattaata taatattaat tataattgat cataaatatc
     4801 gcatccgctt tcgccacacc tggccgaaca ccgctggcta acgctcgata attggcaaaa
     4861 aagtttagtg tgacattgcc atttgcatct acttcctcag tcgggctcgc cgctcccagt
     4921 gcgagccggg agcgatcgct attacgtaat tcgatggcga tggtttgcgc cattgcggga
     4981 tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa aatcgcaact
     5041 gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt ggtatcgcca
     5101 gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc aatatccgca
     5161 gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt aatcgttccc
     5221 aaagggtcag ctgcccatcc actggaaatc cacagtaacc cgcagaaaca gctaaagagt
     5281 gctcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca gcgcgctaaa
     5341 cggcccttcg gtcggttttt gaccggtaat actgataggc ctggcgcgaa gtgtgacatt
     5401 ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt taggcgtgat
     5461 cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg acaccatcgc
     5521 ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg cagcaacatt
     5581 cgtacatttg accgcaatgc tcttggtttg cggcatgaca ccagcaggtc gattacccgg
     5641 ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa caatctgccc
     5701 ggcatttaat tcgcagtttt gcggtacttc aacccggcca ccaaaactaa tggtataaac
     5761 aggtgtaacc aacggatcgt aaggcgttga tgtcacatac acggtaaaca tcacctgtct
     5821 ggggatctcc accatgttaa taaagggacg aataaccttt aaacgaaata ttaaacgaga
     5881 atccatcacc ccgaatggaa ggttttgtga aacgttagga tctgttccca tccgaatata
     5941 attttgcggg gggaactgaa tatccatcac actatcaacc agactcatcg ca
//
LOCUS       DAPHDX010000202         5954 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPHDX010000202 DAPHDX010000000
VERSION     DAPHDX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.205"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(468..1274)
                     /locus_tag="RH058_004211"
     CDS             complement(468..1274)
                     /locus_tag="RH058_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV3139708.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1275..1580)
                     /gene="ccdB"
                     /locus_tag="RH058_004212"
     CDS             complement(1275..1580)
                     /gene="ccdB"
                     /locus_tag="RH058_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDV3139709.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1582..1800)
                     /gene="ccdA"
                     /locus_tag="RH058_004213"
     CDS             complement(1582..1800)
                     /gene="ccdA"
                     /locus_tag="RH058_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDV3139710.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2428..2658
                     /locus_tag="RH058_004214"
     CDS             2428..2658
                     /locus_tag="RH058_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDV3139711.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            2655..3071
                     /locus_tag="RH058_004215"
     CDS             2655..3071
                     /locus_tag="RH058_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDV3139712.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            3146..4711
                     /locus_tag="RH058_004216"
     CDS             3146..4711
                     /locus_tag="RH058_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV3139713.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            4696..5718
                     /locus_tag="RH058_004217"
     CDS             4696..5718
                     /locus_tag="RH058_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HDV3139714.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
BASE COUNT         1443 a         1601 c         1533 g         1377 t
ORIGIN      
        1 agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct
       61 tcctcgctca ctcgagggct tcgccctgtc gctcgactgc ggcgagcact actggctgta
      121 aaaggacaga ccaaacatca tggttctgtg ttcattaggt tgttctgtcc attgctgaca
      181 taatccgctc cacttcaacg taacaccgca cgaagatttc tattgttcct gaaggcatat
      241 tcaaatcgtt ttcgttaccg cttgcaggca tcatgacaga acactacttc ctataaacgc
      301 tacacaggct cctgagatta ataatgcgga tctctacgat aatgggagat tttcccgact
      361 gtctcgttcg cttctcagtg gataacagcc agcttctctg tttaacagac aaaaacagca
      421 tatccactca gttccacatt tccatataaa ggccaaggca tttattctca ggataattgt
      481 ttcagcatcg caaccgcatc agactccggc atcgcaaact gcacccggtg ccgggcagcc
      541 acatccagcg caaaaacctt cgtgtagact tccgttgaac tgatggactt atgtcccatc
      601 aggctttgca gaactttcag cggtataccg gcatacagca tgtgcatcgc ataggaatgg
      661 cggaacgtat gtggtgtgac cggaacagag aacgtcacac cgtcagcagc agcggcggca
      721 accgcctccc caatccaggt cctgaccgtt ctgtccgtca cttcccagat ccgcgctttc
      781 tctgtccttc ctgtgcgacg gttacgccgc tccatgggta ttttcagtgt tgccaccatc
      841 gtctgcagct ggctgacgta ccaggagtca gagagcggaa ccagccggtg agtctgctga
      901 ccggcgggca ttctccccgc cgtcctggca gctttttcgg tccgttgttt cagggtcgca
      961 agctgcacaa acggatacgg aggcgtgagc gaaaaatccc cccgcgtcag cgctagtgct
     1021 tcattaatac gcgcgccggt gttccacaga gtggccagca gcatcttgcg gcgcagatcc
     1081 gggacataat gaagcagggc actcacctcg ggagccagca gatatttcgg cagttcatca
     1141 tgaaccatcg acatctggcg gagtgccaga gctgccggat aatcaatggc aacaggaagt
     1201 tgtgcagact gtccggcaga atacactgcc ggtaccatgg ctgcagactg gctgtgtata
     1261 acggagcctg acatttatat tccccagaac atcaggttaa tggcattttt gatgtcattt
     1321 tctcggtggc tgagatcagc cacttcctcc ccgataacgg agaccggcac actggccata
     1381 tcggtggtca tcatgcgcca gctttcatcc ccgacatgca ccaccgggta aagctcacgg
     1441 gagaccttat ctgacaacag acgtgcactg gccaggggga tcaccatccg tcgcccaggc
     1501 gtgtcaataa tgtcactctg gacatccaca aacagacgat aacggctctc tcttttatag
     1561 gtgtaaacct taaactgcat ttcaccagtc cctgttctcg tcggcaaaag agccgttcat
     1621 ttcaataaac cgggcgacct cagccatccc ttcctgattt tctgcctgcc agcgttcggc
     1681 acgcagacga cgggcttcat tctgcagggt ggtgcttacc agcccggaga tattgacatc
     1741 atatgccttg agcaactgat agctgtcact gtcgacggtg actgtaatgc gctgcttcat
     1801 aatgcacctc atcttacata ctctctgtac accattaagt atacaccagt attacgctat
     1861 aacatgcctc cggcaacgga atattgttat ccagctatta ttaacaccac catcaataac
     1921 tgacctgaaa agtaatcatg acaataataa cctgcaatgt agtaaccatg ataaattaca
     1981 ggatgactgt ttttctatga tccgattttt atcggattca ggattttccg gaagtgaatc
     2041 aggatcagtg aataagcgaa gcgatctttt ctgccgctga tgactggctc gggaacacgg
     2101 gaagaaacac aacatacagt gtcatgtcag cggagcggga caccacatcc tgtgaaagcg
     2161 gaccagcggg cggcagcgaa ggatccgcgg atccggaacc accagaaacc ggatctgcac
     2221 ggggaaagag agaaaataac ataggctgaa catatccata tgcctgacat ctgcatattc
     2281 atggtatacg catatcctca ccatctgcat atgctgaatc tatacatatg cctaacatct
     2341 gcctatacta aacatatgca cacacccggt gcaatgaaca tcattccgga tatgcatcac
     2401 atatccacac ttacccggga gagaatcatg agaaccgtat ccatttttaa aaatggcaac
     2461 aaccgtgcta tccgccttcc acgcgatctg gattttgagg gggtgagcga gctggagatc
     2521 gtccgggaag gggacagcat cattctgcgc cccgtccggc cgacctgggg ctcattcctg
     2581 gagctagaaa aggccgatcc ggactttatg acggagcgcg aggacgttgt cagcgacgaa
     2641 ggacgagtta acctgtgaac aagatctata tgctcgacac gaacatctgc tcgttcatca
     2701 tgcgcgagca acctgaagca ttgcttaagc acctggagca gtcggtgctg cgcggtcacc
     2761 gtatcgtggt ctcggccatc acctactccg agatgcgctt cggtgccacc ggtccgaagg
     2821 cctcgccacg ccacgtgcaa ctggttgacg cgttctgtga gaggctcgat gccgtcctgc
     2881 cgtgggaccg cgccgcggtg gatgcgacca cggagattaa ggtggcactg cgtctcgccg
     2941 gcacgccgat cggtccgaac gacacggcga tagccggaca cgccatcgct gcctgtgcca
     3001 ttctggtgac gaataatgtg agagagtttg agcgggtacc gggtcttgtg ctggaagact
     3061 gggtaagata acgctgtaaa acaaaacagg cggtcaggcg cggccggccg ctctttcagg
     3121 cagcggtata taaaggaata aaaaaatgcc actgatcacg cggcttatgc tgcaaaactt
     3181 caaaaagttt ccggaactgg acctgcgctt cagcaacgat cgtaacattc tggttgggga
     3241 caatgagtcc gggaaaagca ctattctgct ggctcttgac cttgtgctga gtgacagcag
     3301 gcatcgggtt gaggcgctgg gggtggaatc gctcctgtca cagtcagcag tcaggcattt
     3361 tcaggaaggt gagcgccgtg ccgatcagtt acccgtgctg accgctgacg ttttcctgag
     3421 taatggggga gagcctgacc tgaacggcag gcaaaatctg gcgggtatcg atgcggatgg
     3481 tctcagaatg cgcattgctc ccatgatgga ggaatacggt caggatatac accatgtttt
     3541 gcagcaggat cctgataatt ttccctatga atactattcc gtgcagttca gcacattctc
     3601 cggtggtcat ttcgcaggct tcagacgcta tctgcgccat ctgttcttag acagtgcacg
     3661 cattgataac gagcatgccg cacaggagta tacgcgcaca gtttacagcg tcaatgtgcc
     3721 ggtggccgat cggtaccggc ttgaaaacag ttaccggcaa cagaaaatgc acttctgcgc
     3781 ccggcatctg tccgccataa acgacacgct gaaaacgtat cagtttggcg tgcgttccgg
     3841 cgctaaatca ggtcttgagg cgaacctgga cattactgag gacggtattt ccattcgtca
     3901 ccgcggaaaa ggaagacagt gtttcatcaa aacggagttt gcgctgcagc ggcatcagca
     3961 gcagggggga gagatccacg cgctgttgct tgaggagccg gagaatcatc tcagccatgt
     4021 cagtatgaag aggctggtta atcagcttgc cacggaacgg cagacgcagg tgtttattgc
     4081 cacacacagt agccatatct cctcccgcct cgacctacgt aaggctattc tcctcggagc
     4141 tacccggccg gtactgatga atgaactttc cgctgagact gccgccttct tcatgaaagc
     4201 accggataat aacgtgctgg aatttgcgct ggccagacgc gtgctgctcg tggaggggga
     4261 tgctgagttt attctgatcg aggcctttta tcaccgcctg tatggcaggg caccggagga
     4321 tgacggggtt cacatcatcg ctatcggcgg aacgagtttc cgtcgttatc ttgaactggc
     4381 ccgtcttctg gaaaatcgtg tcgcggccct tcgcgacaat gacggcaact atcagcaaaa
     4441 ttgcgatgaa cgctatgctg acgtgctttg ttcccgctcc cgggtatttg ctgaccacga
     4501 caacagccgc tccacatttg aaatctgcct gtatcaggat aatgccgacc tgtgtgatgc
     4561 gcttttccgc ggaacccgcc ggacactcac ggttcaggat tacatgctgg ccaataaggc
     4621 agaagcagca ttccagctgt tacagttgca cgccgaaaag ctgaccgttc ctgattatat
     4681 ccaggaggca ctggcatgga taagagagtg atatttgcgg ttgccggctc gggtaaaacc
     4741 acgctgctga tacgtcggtt aagagaggac cggcggacgc tgatcctgac cttcaccgta
     4801 aataatgaag cacatctgcg ggcgcagatt atcagacgct ttggctacat accgtacggg
     4861 atccgtgtga tgacctggtt tgagtttctg cacggtttct gttttcgccc tttcctgcag
     4921 gagcaactgt catcgcgtgg tctgagcttc aaccagcccc cttccagaat accgcgcacg
     4981 aatatccggc attatcagga tcccgccgga cggctgtatc accggcggct cgcacacctg
     5041 ctgacagccc gggggttgct gccagatatc cgtacccgac ttgcccgtta ctacgatgag
     5101 ttgttcgttg atgaagtcca ggactttgcc ggccatgact ttaattttct gctggagcta
     5161 tgccgcgcgg aaatctcagt gctgtgctgc ggggattttt atcagcatac ctttgacaca
     5221 agccgcgacg gtaacgtcaa tgctacgctg catgaagaca taacccgata cgaggctcgc
     5281 ttcagggctg cgggtatcat ggtggattgt gagacgctca gccggacatg gcgctgttca
     5341 gctacagtgt gtgagttcat taccggacaa ctgaacattc gcatttcggc tcatggaaca
     5401 cataccaccc agattgaaat cgtcacggat gaagcgcgtt cagcggccct gcacgcggac
     5461 aacacgatga tcaagctctt ctatcgcgaa catcaccgtt acggatgcca ctcaatgaac
     5521 tgggggggca gtaaaggact cgaccacttt caggacgtct gcatcgtgat gggggccaac
     5581 cactggatgc gcctcatcca acagaagctg gcagcacttc caccatcatc ccgtaacagg
     5641 ctatacgtgg cctgctcacg ggcacggggc aacatctatt ttattccgga aagccatctt
     5701 cggagattca gaaactaagt cagcggcttc tcaactgggc acaagccggt aaaaagtagt
     5761 caacgacaaa acttcaggcc gaaaaatgag gctccggttt cgccggaaac cggacctgcg
     5821 tggagggaaa cccgcagatg atatgatatt tatcattaat cgcataataa atcctggcct
     5881 gatatttaca ggcagagaaa taaatcattc gatatatgat tttatcgtca tcagccatta
     5941 accccccagg aagc
//
LOCUS       DAPHDX010000203         5943 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPHDX010000203 DAPHDX010000000
VERSION     DAPHDX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.206"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            140..586
                     /locus_tag="RH058_004218"
     CDS             140..586
                     /locus_tag="RH058_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV3139715.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQPLSS"
     gene            597..2048
                     /locus_tag="RH058_004219"
     CDS             597..2048
                     /locus_tag="RH058_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV3139716.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2038..3108
                     /locus_tag="RH058_004220"
     CDS             2038..3108
                     /locus_tag="RH058_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3139717.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3108..4856
                     /locus_tag="RH058_004221"
     CDS             3108..4856
                     /locus_tag="RH058_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV3139718.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4906..>5943)
                     /locus_tag="RH058_004222"
     CDS             complement(4906..>5943)
                     /locus_tag="RH058_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV3139719.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQ
                     GPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREY
                     NEMVDDYNSLR"
BASE COUNT         1386 a         1511 c         1500 g         1546 t
ORIGIN      
        1 taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa attgtcattg
       61 tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg attaaaatat
      121 attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc tgaatattca
      181 gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac aaaacggttt
      241 tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa tgcactctac
      301 tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta aacaaccgtt
      361 cgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg gcgaagacgt
      421 caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg tcaaaatcat
      481 cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc aacagggcga
      541 cgccgaccag gtgcttgcct tgttaaatca acccctcagc tcataaggaa gtggcgatgg
      601 agtcatcctt acgtattgtc gcgatcacca actgccccgc cgggatcgct cacacctaca
      661 tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata aaagtggaaa
      721 ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc gctgccgatt
      781 acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg tttgccggga
      841 agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag attttcagcg
      901 aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt ggcaaacagg
      961 aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg gcgctgccgt
     1021 ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag ttcggtttgc
     1081 cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa tccatcggtt
     1141 accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg tcgattgccg
     1201 ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac aaagcgctgc
     1261 tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg gcgattggct
     1321 atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg ctgctcggtt
     1381 caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac tacgttatcg
     1441 ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc attcctccat
     1501 caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat atgggcggcc
     1561 ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac atttatgact
     1621 ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt cttgcgacgt
     1681 tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt gccattgtgg
     1741 tcggtgccac agtcgcaacc gaacctgcta ttccttacgc tctggcagct ccgttaccga
     1801 tgattaccgc caatacgctg gcgggcggta ttaccggcgt gctggtgatc gctttcggaa
     1861 tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg atgtcgccgg
     1921 taggttcgtt ttatctggtg ctggcgattg gcctcgcgct aaacatttca ttcattatcg
     1981 tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag cttgtccatg
     2041 aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga tgaacaggaa
     2101 gttcgcgaca tcctgataaa cacgctggaa ccttgcgtta atgaaatcac ctttgatggt
     2161 ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt tgtcgggcat
     2221 atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct gcgcttcacc
     2281 accattggcg gctggtggaa tcagtcgatg ctcaaccacc gggtaaccat acgcacacac
     2341 aagggattta aaatccctgg tgtgattggt tccgtcgcgc ctcatgcgtt aacggaaaag
     2401 caaaagcaac aaccgctgtc atttgatgag atgttcattg atattggcgc gaacagtcgc
     2461 gaagaagcgg aaaagcgcgg cgttgaaatt ggcgatttta ttagcccgga agccaatttt
     2521 gcctgctggg gcgaagataa agtagtcgga aaggcgctgg ataaccgcat cggctgcgcg
     2581 atgatggcgg agctactaca gacagtaaat aacccagaaa ttacgctgta cggcgttggc
     2641 agtgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca cattaaaccg
     2701 gatgtggtga ttgtgctgga taccgctgtc gcaggtgatg ttccgggcat tgataacatt
     2761 aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa gcgctacttc
     2821 cccaaccaga aactggtagc agtgttaaaa aactgtgccg cacataacga tttaccgctg
     2881 caattttcca ccatgaaaac cggagcgacg gatggcgggc gctacaacgt gatgggcggc
     2941 gggcgtccgg ttgtcgcgct gtgtctgcca actcgttatc tgcacgctaa cagcggcatg
     3001 atttcaaaag ccgattacga tgctctgctc acgctgatac gggattttct gacgacctta
     3061 actgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg ctaaacgaac
     3121 gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctgga gagctggcgc
     3181 aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc aacttcaccc
     3241 ttaacggcaa agcccgcatt ttcgccagtg gtagtgcggg ctatcagctg gaaatcttcg
     3301 agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg ctgctggcgc
     3361 tattattact aaataccttt actccccgtg cgcaactcgc ctcggcgctt aatttgccag
     3421 aaacgtgggt agcagaacgt ctgccccggt taaaacagcg ttatgaacgc acttgttgcc
     3481 tggccagtcg ccccggtttg ggccatttca ttgatgaaac agaagagaag cgcgttatct
     3541 tgctggcgaa cttgctacgc aaagatccgt ttttaattcc gctggcgggc ataacacgag
     3601 acaaccttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg ctgatgcagg
     3661 gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcag cttactgaca
     3721 tatggccgca atatcccggt aacgagataa aacaaatcgt tgaacagtgc ggcctgtttc
     3781 tcggtgataa cgctgtaaga accctgacgg gtttgataga gaaacagcat cagcaagcgc
     3841 aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccaggc atcgcgtcat
     3901 tgaatattat tgatacgcag ttggttgaga atattaccgg gcatttatta cgttgccttg
     3961 ccgcaccggt gtggattgct gagcaccgcc aaagcagcat gaataacctg aaagccgcct
     4021 ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa cagctcgata
     4081 ttcccctttt cgacagcgat ctgatcggtt tgtattttgc ctgtgcgctg gaacggcatc
     4141 aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc actattaatc
     4201 agctcgccat tgagcgcgat gttttaaatt gtcgggtaat tattgcccgt agcttaagcg
     4261 aacttgttgc cattcgcgaa gagattgagc cgttattgat cattaacaat agccattatt
     4321 tactggaaga cgcggtaaat aattacatca ccgtaaaaaa tatcattacg gctgccggta
     4381 tcgaacaaat aaaacatttt ctggcgacgg catttattcg ccagcaaccg gagcgttttt
     4441 tctctgcccc cggaagtttt cattattcga atgtacgcgg tgaaagctgg caacatatta
     4501 cccggcaaat ttgtgcgcaa ttagtggcac aacaccatat taccgccgat gaagcacaac
     4561 gcatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc gccatcccac
     4621 attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc gcccacccga
     4681 tcgaggtgaa taacgaaatc attaaccatg tgttgattgc ctgcgccgcc gccgatgcgc
     4741 gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat ccggcagagg
     4801 ttattgccgg gttaacagga tatgaggcat ttatggagtt acttcacaag gggtgaactg
     4861 tgtcggatgc gccgcatccg acactgcatt tatcatcaaa aacgtttaac gcaggctatt
     4921 atagtcatca accatttcgt tgtactcgcg taatgctctc gggaaagagt cgtcgaccat
     4981 ccagcttgag tttgcgtcct gcagttgctc tttatcccag tcagagtacg ggactttatc
     5041 tctgatgcgg cgaacgtatt ttttagcctc gagttgatac tggcctgccg aattaataaa
     5101 cgagaaattc atgccgcctt tatccgcttc tttggcctgc gccactagcg tttccagttc
     5161 agacactttc ttcattgccg catcaacatc aaacttctct tgcgatatca gattattaat
     5221 ttgtttcgct gaaatcatga ctgccagaga gtaatagtgg aatgtttttc cttctctttc
     5281 ttcaatattt ttcagttccg caagctggcg cttatcgtta atttcctgaa tcgccgcatg
     5341 ataagattct gctacagctt caaaggcttc cagatttttt aagaatgtct ggtgcagcgt
     5401 tttccctttc gcaaacgcat cgtctttata attttcctgg gtgtaatatt tatccatttc
     5461 gttaatggta ttacccaacg ccacggcagc atcgatataa ctcaccgcca cgccatcaat
     5521 aggttgcagc gcaggcgtta acgccacagc actttttacg cctttttcac actcagcgag
     5581 gttggattca ctaatgccgt agatgccata aacagtacgc tcttcaccgg taggcccctg
     5641 tttaaaatct ttcagccagt cagcataacg cgccagactg ctctgtacag ggatctgtaa
     5701 cttgttatag cacttgatat agacatccat tttttcgtcg aaaaccgctt gttcatcgac
     5761 caccggttgc gcaggtgttt caggtttagc ttctgctttt gcaacgggag cttcaggagc
     5821 tggtgcggca ggttgactat tggcaggcgg gggcgtttct gtttcggctt ttttatcatc
     5881 acaacccgtc agaccgaggg ccatgatggc gactataata gcggaagata ataaattcct
     5941 ttt
//
LOCUS       DAPHDX010000204         5917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPHDX010000204 DAPHDX010000000
VERSION     DAPHDX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.207"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            92..1243
                     /gene="yiaY"
                     /locus_tag="RH058_004223"
     CDS             92..1243
                     /gene="yiaY"
                     /locus_tag="RH058_004223"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV3139720.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1408..2946
                     /gene="aldB"
                     /locus_tag="RH058_004224"
     CDS             1408..2946
                     /gene="aldB"
                     /locus_tag="RH058_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV3139721.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3165..3308)
                     /locus_tag="RH058_004225"
     CDS             complement(3165..3308)
                     /locus_tag="RH058_004225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139722.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3491..3814
                     /locus_tag="RH058_004226"
     CDS             3491..3814
                     /locus_tag="RH058_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV3139723.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3820..4956
                     /locus_tag="RH058_004227"
     CDS             3820..4956
                     /locus_tag="RH058_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3139724.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4953..>5917)
                     /locus_tag="RH058_004228"
     CDS             complement(4953..>5917)
                     /locus_tag="RH058_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3139725.1"
                     /translation="QLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFESKV
                     GLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGI
                     ITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVA
                     SFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVR
                     DYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLV
                     AIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1465 a         1416 c         1543 g         1493 t
ORIGIN      
        1 ttaattttga taatcaataa taatgatcat gttttcgaat gaaaatcctc agtaagctgc
       61 ccaccctttt ttacactttc aggagtgtgt tatggcatct tcaactttct ttattccttc
      121 tgtgaatgtc atcggcgctg attcattgac tgatgcaatg aatatgatgg cagattatgg
      181 atttacccgt accttaattg tcactgacag tatgttaacg aaattaggta tggcgggcga
      241 tgtgcaaaaa gcactggaag aacgcaatat ttttagcgtt atttatgatg gcacccaacc
      301 taaccccacc acggaaaacg tcgccgcagg tttgaaatta cttaaagaga ataattgcga
      361 tagcgtgatc tccttaggcg gtggttctcc acacgactgc gcaaaaggta ttgcgctggt
      421 ggcagccaat ggcggcgata ttcgcgatta cgaaggcgtt gaccgctctg caaaaccgca
      481 gctgccgatg atcgccatca ataccacggc gggtacggcc tctgaaatga cccgtttctg
      541 catcatcact gacgaagcgc gtcatatcaa aatggcgatt gttgataaac atgtcactcc
      601 gctgctttct gtcaatgact cctctctgat gattggtatg ccgaagtcac tgaccgccgc
      661 aacgggtatg gatgccttaa cgcacgctat cgaagcgtat gtttctattg ccgccacgcc
      721 gatcactgac gcttgtgcac tgaaagccgt gaccatgatt gccgaaaatc tgccgttagc
      781 cgttgaagat ggcagtaatg cgaaagcgcg tgaagcaatg gcttatgccc agttcctcgc
      841 cggtatggcg ttcaataatg cttctctggg ttatgttcat gcgatggcgc accagctggg
      901 cggtttctac aacctgccac acggtgtatg taacgccgtt ttgctgccgc atgttcaggt
      961 attcaacagc aaagtcgccg ccgcacgtct gcgtgactgt gccgctgcaa tgggcgtgaa
     1021 cgtgacaggt aaaaacgatg cggaaggtgc tgaagcctgc attaacgcca tccgtgaact
     1081 ggcgaagaaa gtggatatcc cggcaggcct acgcgacctg aacgtgaaag aagaagattt
     1141 cgcggttctg gcgactaatg ccctgaaaga tgcctgtggt tttactaacc cgatccaggc
     1201 aactcacgaa gaaattgtgg cgatttatcg cgcagcgatg taatcatcat ttccacaacg
     1261 gctggcaaat tgtcagccgc tttttcaact atctctgtaa cccttgcccg taaattcgtg
     1321 atagctgtcg taaagctgtt accgactggc gaagatttcg ccagtcacgt ctacccttgt
     1381 tatacctcac accgcaagga gacgatcatg accaataatc ccccttcagc acagattaag
     1441 cccggcgagt atggtttccc cctcaagtta aaagcccgct atgacaactt tattggcggc
     1501 gaatgggtag cccctgccga cggcgagtat taccagaatc tgacgccggt gaccgggcag
     1561 ctgctgtgcg aagtggcgtc ttcgggcaaa cgagacatcg atctggcgct ggatgctgcg
     1621 cacaaagtga aagataaatg ggcgcacacc tcggtgcagg atcgcgcggc gattctgttt
     1681 aagattgccg atcgaatgga acaaaacctt gagctgttag cgacggctga aacctgggat
     1741 aacggcaaac ccattcgcga aaccagtgct gccgacgtgc cgctggcgat tgaccatttt
     1801 cgctatttcg cctcgtgtat tcgggcgcag gaaggtggga tcagtgaagt tgatagcgaa
     1861 accgtggcct atcatttcca tgaaccgtta ggcgtggtgg ggcagattat cccgtggaac
     1921 ttcccgctgc tgatggcgag ctggaaaatg gctcccgcgc tggcggcggg caactgtgtg
     1981 gtgctgaaac ccgcacgtct taccccgctt tctgtactgc tgctaatgga aatcgtcggt
     2041 gatttactgc cgccgggcgt ggtgaacgtg gtcaacggcg caggtgggga aattggcgaa
     2101 tatctggcga cctcgaaacg catcgccaaa gtggcgttta ccggctcaac ggaagtgggc
     2161 caacaaatta tgcaatacgc cacgcaaaac attattccgg tgacgctgga gctgggcggc
     2221 aaatcgccaa atatcttctt tgctgatgtg atggatgaag aagatgcctt tttcgataaa
     2281 gcgctggaag gctttgcact gtttgccttt aaccagggtg aagtttgcac ctgtccgagt
     2341 cgtgccttag tgcaggagtc aatctacgaa cgctttatgg aacgcgccat ccgccgtgtc
     2401 gaaagcattc gcagcggtaa cccactcgac agcgtgacgc aaatgggcgc gcaggtttct
     2461 cacgggcaac tggaaaccat cctcaactac attgatatcg gtaaaaaaga gggcgcagac
     2521 gtgctcaccg gcgggcggcg caagctgctg gaaggtgaac tgaaagacgg ctactacctc
     2581 gaaccgacga ttctgtttgg tcagaacaat atgcgggtgt tccaggagga gatttttggc
     2641 ccggtgctgg cggtgaccac cttcaaaacg atggaagaag cgctggagct ggcgaacgat
     2701 acgcaatatg gcctgggcgc gggcgtctgg agccgcaacg gtaatctggc ctataagatg
     2761 gggcgcggca tacaggctgg gcgcgtgtgg accaactgtt atcacgctta cccggcacat
     2821 gctgcgtttg gtggctacaa acaatcaggt atcggtcgcg aaacccacaa gatgatgctg
     2881 gagcattacc agcaaaccaa gtgcctgctg gtgagctact cggataaacc gttggggctg
     2941 ttctgatata agaagctggt cgcattgggt attcattgcc tgatgcgacg cttacgcgtc
     3001 ttatcatgcc tacgggaacc tgaccgtagg ccggataagg cgtacacgcc gcatccggca
     3061 taaacaaagc gcacgttgtt acaatttacg ttttaaacct acgtgcgcaa taacccagac
     3121 tacgccaaca gaagtgcgtt tctggggcgt gttttgtcag gagtttaatc tcgcgtttcg
     3181 atggtgagcg ggcgaaccag gccgtaaatc accattggtg atccgaggcc aatcagcaat
     3241 aaacggtaga taattgaagg tttaatacgc ttaatctgta ctgtggatgt tttactggag
     3301 ttattcatta gtttggccaa tagttccact atttcaatat gataaacctc gtcctgttga
     3361 aaaaccagac agcacattta agaaagtata taaaaaacaa tatgtcgtta tataaataag
     3421 gaaattgcac tgcatcatgg catttcataa tatgcgttaa ttgttctttt ttatattctt
     3481 tgagggaaat atgttcctgg actattttgc actgggagtg cttatttttg tatttctggt
     3541 gatcttctat gggatcatta ttttacatga tattccctac ctgattgcca aaaaacgtaa
     3601 tcatcctcat gccgacgcca ttcatgttgc tggttgggtg agtcttttta cgttacatgt
     3661 tatctggccg tttttgtgga tttgggccac gctttaccgc ccggagcggg gatggggaat
     3721 gcaaagccat gattcatccg ttatgcaact gcaacagcgc attgccgggc tggagaaaca
     3781 gctcgccgac atcaaatcct cttctgccga gtaatactta tggatttact gattattttg
     3841 acctatgtgg cttttgcatg ggcaatgttt aagatcttca aaattcccgt aaataaatgg
     3901 accattccca cagcggccct gggtggaata tttattgtca gcggtctaat tctgttaatg
     3961 aactataacc atccgtatac cttcaaagcg caaaaagcgg ttatttctat tcctgttgtc
     4021 ccacaggtga caggcgtggt gatcgaagtg acggataaga aaaatacgct gattaaaaaa
     4081 ggtgaggtgc tatttcgact ggacccgacg cgttatcagg cgcgggtgga tcggctgatg
     4141 gcggatatcg ttaccgcaga acataaacag cgggcgttgg gcgcagagtt agatgagatg
     4201 gcggcgaata ctcagcaggc aaaggccacg cgggataaat tcgctaaaga gtatcagcgt
     4261 tacgcacgcg gcagccaggc gaaagtaaac ccgttttcag aacgcgatat tgatgtggcg
     4321 cggcaaaatt atctggcgca ggaagcctcc gtaaagtcat cggcggcgga acaaaaacag
     4381 atccagagcc agctggatag cctggtgttg ggtgaacatt ctcaaatcgc cagcctgaaa
     4441 gcgcagctcg cggaagcaaa atataacctt gagcagacga tagtgcgtgc gccgagcgat
     4501 ggttatgtga cccaggtgct gattcgtccg ggtacctatg ccgcgtcgct gccgctacgt
     4561 ccggtgatgg tgtttatacc cgatcagaaa cgacaaattg tggcgcagtt ccgtcagaac
     4621 tccttgctgc gactggcccc tggcgacgat gcggaagtgg tgtttaacgc tctgccaggc
     4681 aaagtgttca gcggtaagct ggcagccatt agtccagctg ttcccggcgg agcttatcag
     4741 tcgaccggca ccttacagac gttaaacaca gcgccgggtt cagatggcgt tatcgcgacc
     4801 attgaactgg atgagcacac tgatttgagc gctttaccag acggtattta cgcccaggtg
     4861 gcggtctact ctgatcattt cagccatgtc tcggtgatgc gcaaagtgct gttacgcatg
     4921 accagttggg tgcattacct ttatctcgat cattaatctt cgctttccgc cgcttgttca
     4981 ggccgcagtt gctgcgactg agcaagctgg tggcgaattt cacgcagcat ttcatccagc
     5041 acatactgga cccgccaggg ctggtattcc cgtttacgga agatagccac cagatccagc
     5101 caccattcat acgggtgctg aaagcagggc accagcgtgc cgtcttgcag atcgctctgc
     5161 acacttttgt ctggcgcaaa cacaatcccc aggtgatttc gtgccagctc cagcgccgat
     5221 tgcgtgttat cgcaaacgta atttcccttc acgcgataat cccgcacctc tttactcccg
     5281 gcgacgttga agcgccagat attcgcgtca tcaatcatca tcgagtcgat caaaatacag
     5341 gagtgatgtt cgagttcgtc aggacggcta ataggatgtt tctcaagata gcgctggctg
     5401 gcataagcgg ttacggcata ttgggtaata aaactggcaa ccagcgattc atcttttggt
     5461 ggagcgtagc tgattaaaac atcacaatca tcaggaaatt cgacgccttc ataaaaggca
     5521 ttacgctcaa gattgcaggt ttttagcgat aaggtgatat caccgatatc tttaatttta
     5581 tcgattacat gtttggataa ataggtaatt atgccggttg gggcgtagat ggtgacccga
     5641 ccacgtttct catgcttata atctgcaata aaattattaa gctgctcgtt tctgtccagc
     5701 atgtcgttga tgtacggtaa cagcgcggta ccaaaaggtg tgagcatcag ctgccgggtg
     5761 gttcggtcaa agacttttaa acccactttt gattcaaaat cagcaaggta tttgctgacg
     5821 ttggcctgtg cgatgccaag tacagtcgcg gcatggctga tattttcact ggcagcgatt
     5881 accgagataa tttttaactc ccggtactta agttgta
//
LOCUS       DAPHDX010000205         5873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPHDX010000205 DAPHDX010000000
VERSION     DAPHDX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.208"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(381..602)
                     /locus_tag="RH058_004229"
     CDS             complement(381..602)
                     /locus_tag="RH058_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139726.1"
                     /translation="MHFRKMKVVFLFIHILAVVMISVMTVHLCSMAVEKVPEECDIPV
                     RVAALVVLWLVFSGLIYWLFTAFMKTDVM"
     gene            complement(617..808)
                     /locus_tag="RH058_004230"
     CDS             complement(617..808)
                     /locus_tag="RH058_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139727.1"
                     /translation="MSDFVRHFSVKDKKEDVVMQDMHFLLEDFICAHISRKSGRVPGR
                     LRQRMDKLRLQRIRKDRTG"
     gene            complement(921..1955)
                     /gene="f17g"
                     /locus_tag="RH058_004231"
     CDS             complement(921..1955)
                     /gene="f17g"
                     /locus_tag="RH058_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17G fimbrial adhesin"
                     /protein_id="HDV3139728.1"
                     /translation="MTNFYKVFLAVFILVCCNISQAAVSFIGSTENDVGPSPGSYSRT
                     HAMDNLPFVYNTGNNIGYQNANVWRISKGFCVGLDGKVDLPVVGSLDGQSIYGLTEEV
                     GLLIWMGDTNYSRGTAMSGNSWENVFSGWCVGANTASTQGLSVRVTPVILKRNSSARY
                     SVQKTSIGSIRMRPYNGSSAGSVQTTVNFSLNPFTLNDTVTSCRLLTPSAVNVSLAAI
                     SAGQLPSSGDEVVAGTTSLKLQCDAGVTVWATLTDATTPSNRSDILTLTGASTATGVG
                     LRIYKNTDSTPLKFGPDSPVKGNENQWQLSTGTETSPSVRLYVKYVNTGEGINPGTVN
                     GISTFTFSYQ"
     gene            complement(1957..4425)
                     /locus_tag="RH058_004232"
     CDS             complement(1957..4425)
                     /locus_tag="RH058_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3139729.1"
                     /translation="MLSVPLWAAEDAEFDPSFLSRTPGSSVIDVRRFTHGNPVPSGNY
                     YSDIYLNGEWKGNAELSFRDMSSSQEESVLCVTPELLSILDVSPEAVAREPDTGNGRC
                     MPFSAVVPDAKIRFDLSLLRLNVEIPQAMLVSRPRGYISPSQWQTGVPAAFVSYDVNH
                     WRYRTPDMENNQSYLGLRAGFNAGGWAFRHRGTESWSDGHAEGYRSIETNLQHDIAML
                     RAQLTLGDFTTTGELMDSNSLRGIRLASDDRMLPGSLRGYAPVVRGMAGSNARVTVRQ
                     NGNIIYETTVPAGPFSISDLYPSGYGGDLTVTVTESDGQTRSFIVPFASVAQLVRPGF
                     SRWQVAAGRYRYGNRTFSDTVFQGTLQYGLTNDITLNTGMSTAPHYLSGLGGVAFNTP
                     LGAVATDITLARTTFPGSDTTHRGYSLHAGYSARIPETSTNVTLAAYRYSSRDFWSLR
                     DAILTRNRDVINDSSVRSATGYRPRNQLQLTVNQSLGEGWGNLYLTGATYHYWGHTGT
                     FNEYQAGYNNVWKKMSYQIGFSQTRRSGDYHHDNRFFFSFSLPLSDTGRTLLSTTVNV
                     SDKNSNSIQSSISGVSGDDNQLSYGVSLNTQENGPTGYAVNGSYRSPVATLQATAGND
                     SRHNRQISAGISGAIVAHPFGVTASNDLGDTFTVIHADGAGGAVINNAPGNRLDPWGN
                     GIVPYVTPYEKNYISIDPTALAADVELSATEREIIPRANSTTMVTFTTKTGKAMLFDV
                     KMPDGTPPPMAAEALTADGKSAGYIAQGGRLFVRDLDAEKGMLRIVWGSGQNDSCVIH
                     YSASGENARGNAVPENHHVVCMRQ"
     gene            complement(4508..5230)
                     /locus_tag="RH058_004233"
     CDS             complement(4508..5230)
                     /locus_tag="RH058_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3139730.1"
                     /translation="MKHILLFIFTVLLSLPSYGSVVIMGTRVIYPAEQKSINVRLNNG
                     DSTPSLIQAWLDTGDPSSPPDSVRVPFIITPPVFRMEPLSGQTMRIMYTGEKLPADRE
                     SLFWLNVLDIPAKPSFAGKSEKAQGYNYLQFAVRSRIKFFFRPDGLPFSPDDAYKKVT
                     WQLTGGDTLQASNPTPYFITFSTVSVGQGAQKVSSTQGGMAAPFSSVTFRLKQKVSPS
                     GKVEWTVVNDNGGYQKGESVLK"
     gene            complement(5298..5843)
                     /gene="f17a"
                     /locus_tag="RH058_004234"
     CDS             complement(5298..5843)
                     /gene="f17a"
                     /locus_tag="RH058_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021578761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17A fimbrial adhesin"
                     /protein_id="HDV3139731.1"
                     /translation="MRKIQFILGILAAASSSATLAYDGTITFNGKVVDQTCSVTTESK
                     NLTVTLPTVSSNSLTSNGEVAGLTPFTISLEGCTSPASAGAENVKAYFEPNATTDYDS
                     HNLNIASGSGNATNVQIQLLNADGTTPILLGTDSAGQAVTPVAINGTNVTLRYNARYY
                     ATGQATAGNVSATVNYTIAYQ"
BASE COUNT         1586 a         1538 c         1281 g         1468 t
ORIGIN      
        1 agtgatggtg tttttatgaa gcaatttaaa taatacagat aactgtaatt tatcattcag
       61 acgaagagat ttgatttaca cggtaagagc atatggcaca ttacaacagg ggtctctgtg
      121 aatatataca gaaaacatat ctcataaaac ccgggaaatg ccccatgaat acaggacagt
      181 gaaaacaggc atgataatca ggaaagccat gcctgataca gaaaaagtta cctgcgggga
      241 taatgcatgc gtattttcat ggaaggagat cactcaataa cttccatcga gagcgggtaa
      301 taactcaccg atctccttca cggtaataag caatgcacaa ccaggcactt actggccggt
      361 tatccatgtc tatattatat ttacatcacg tcagttttca taaaggctgt gaacagccag
      421 taaatcaggc cactgaatac cagccacagc acaaccaggg ctgcaaccct cacagggata
      481 tcacactctt ccggcacttt ttccacagcc atactgcaca gatggactgt cataacagaa
      541 atcatcacaa cagcaaggat atgaatgaaa agaaaaacga ctttcatttt ccggaaatgc
      601 atatacaaac cctgactcag cctgtcctgt ctttcctgat cctctgtaat cttaacttat
      661 ccatgcgctg acgaagtctg cccggaacac gcccgctctt tcttgaaata tgagcacaga
      721 taaaatcttc aagcagaaaa tgcatgtcct gcattaccac atcttctttt ttgtctttca
      781 ctgaaaaatg cctgacaaaa tcgctcatga aaatgtttct gaaacgctgt tatccagctt
      841 cagaaaccgg tactgccttc accaacacaa atcattttgt tgtcactgtc tgacctgttc
      901 tcccctcccg gaactcgctg ttactgatag gaaaatgtaa atgttgatat tccgttaacc
      961 gtacccggat taattccctc accagtattc acatacttta catacaaccg gactgagggt
     1021 gacgtttccg ttcctgttga taactgccac tggttttcat ttccctttac cggcgaatca
     1081 ggtccaaact tcaggggcgt actgtcagtg tttttgtata ttctcagccc gactccggtt
     1141 gcagtcgatg cccccgtcag tgtgagtata tcgcttctgt tggacggtgt ggtcgcatca
     1201 gtcagtgttg cccatactgt tactccggca tcacactgta atttcagtga tgttgtcccg
     1261 gcgacaactt catcaccgga tgatggcagt tgtccggcag aaattgcagc caggctgaca
     1321 ttgactgcgg aaggtgtcag taatctgcac gatgttactg tgtcattcag cgtaaatgga
     1381 ttcaggctga aattcactgt ggtctgaaca ctgcctgcag atgaaccgtt atagggcctc
     1441 attctgatac tcccgatgct ggtcttctgt acactgtatc gcgcagagga atttcttttt
     1501 aaaattacag gtgttacacg aacagacagt ccctgggttg atgcagtatt agctccgaca
     1561 caccatccgg aaaagacatt ttcccatgag tttccgctca tcgcggtacc cctggaataa
     1621 ttcgtgtccc ccatccatat aaggagcccc acctcctctg tcagcccata aatactctgc
     1681 ccgtcaagac tgccaaccac gggtaaatcc actttcccgt ccagcccgac acaaaatccc
     1741 ttagaaatac gccagacatt tgcattctga tatccaatgt tgttaccggt attatagaca
     1801 aatggcaggt tatccattgc atgagttctg gaataagagc ctggagatgg tcccacatcg
     1861 ttttccgtac tgccaataaa tgaaactgcc gcctgactga tattgcagca aaccagaatg
     1921 aatacagcca gaaagacctt ataaaaattt gtcatattat tgcctcatgc atactacatg
     1981 atggttttcc ggaacagcat ttcccctggc attttctccg gacgcactat agtgaatgac
     2041 acaactgtca ttctgcccgc ttccccagac tatcctgagc atcccttttt cagcatccag
     2101 gtcacgtaca aataaccggc ctccctgagc aatgtagcct gcagatttcc cgtctgctgt
     2161 taatgcttcc gcggccatcg gcggcggcgt tccgtccggc atcttcacat caaacaacat
     2221 ggctttcccc gtttttgttg tgaaggtcac catggtggta ctgttggccc ggggaataat
     2281 ttcccgctct gttgcagaca gttccacatc cgcagccagc gctgtcgggt cgatactgat
     2341 ataattcttt tcgtaaggtg tgacatacgg aacaatgccg ttaccccagg ggtccagccg
     2401 gttacccggc gcattattaa tcaccgcccc accggcacca tcagcatgaa taacggtaaa
     2461 tgtgtcacca aggtcattac tggccgtcac accaaacgga tgcgccacta ttgccccgga
     2521 tataccggca gatatctgac ggttatggcg actgtcatta cctgccgtgg cctgcagggt
     2581 ggccaccggt gaacgataac tgccattcac cgcataccct gtcggtccgt tttcctgggt
     2641 gttcagggac acaccatatg acaactgatt gtcatccccg ctgacaccac tgatactgct
     2701 ctgaatgctg ttactgtttt tatcgctgac attcactgtc gtggacagca acgtccttcc
     2761 cgtatctgaa agtggcaggg aaaaactgaa aaagaaccgg ttatcatgat gataatcacc
     2821 actccgtcgg gtctgggaaa agccaatctg gtaactcatt tttttccaga cgttgttata
     2881 accggcctgg tactcattga atgtccctgt atgcccccag taatgataag tggctccggt
     2941 cagatacagg tttccccatc cctcaccaag actctggttc acagtcagtt gcaactgatt
     3001 ccttggccgg taaccagttg cactcctgac cgatgaatca ttaatcacat cacgattcct
     3061 ggtcaggatg gcatcccgaa gactccagaa gtcacgggat gaataacggt aagccgccag
     3121 cgtcacattt gtactggttt ccggtattcg tgcactgtaa ccggcatgca ggctgtagcc
     3181 gcgatgtgtc gtgtcagaac ccgggaaagt cgttctggcc agcgtaatat ccgtggcaac
     3241 agcccccagt ggtgtattga atgccacacc acccagccct gacagataat gaggtgcggt
     3301 actcatcccc gtattcaggg tgatatcatt tgtcagcccg tactgcaggg tcccctggaa
     3361 tacggtatcg ctgaatgtcc tgttaccgta acgataacgc ccggctgcca cctgccagcg
     3421 actgaacccc gggcgaacca gttgtgcaac cgaagcaaac ggcacgataa aactcctggt
     3481 ctgtccgtct gactctgtca cagtgactgt caggtcacca ccatatccac tgggataaag
     3541 gtcactgatg gagaaaggcc ctgccggcac ggtagtttca taaataatgt taccgttctg
     3601 ccggacagta accctggcgt tacttccggc catcccccga accacagggg cataaccacg
     3661 caacgaaccc ggcagcatcc ggtcatcaga agccagtctt attcccctga ggctgttact
     3721 gtccataagc tcacctgtgg tggtgaaatc ccccagtgtc agttgcgcac gcaacatggc
     3781 tatgtcgtgc tgaaggttcg tttcaatact ccggtaacct tctgcatgac cgtcactcca
     3841 gctttctgtc ccccggtgac gaaatgccca gcctcctgcg ttaaagccgg cacgaagacc
     3901 aagataactc tgattatttt ccatatcggg cgtccggtaa cgccagtgat tcacgtcata
     3961 actgacaaag gcagccggca cccccgtctg ccactgagaa ggtgatatgt atccccgtgg
     4021 acgggaaacc agcatggcct gcggaatttc aacattcagc cgcagcagtg acaggtcaaa
     4081 acggattttt gcatcaggta caactgcaga aaatggcatg caacggccat ttccggtatc
     4141 cggttcacgg gcaactgcct cgggtgacac atccagaatg gataacagtt ccggcgtcac
     4201 acacagaaca ctctcctcct gtgaagagga catatcacgg aatgacaatt ccgcatttcc
     4261 tttccattca ccgttcagat aaatgtctga ataataattc cctgagggaa caggattccc
     4321 gtgcgtgaaa cgacggacat caatcacact gctccccggc gtgcggctca gaaaactcgg
     4381 gtcaaattct gcatcctctg ccgcccacaa aggcacagac agcatataca gtcccgacag
     4441 acaacataaa taaatgacac agtacttcag accggataaa gaataatgac tcacttacgg
     4501 gcccttatta tttcagaacg gattcacctt tctggtaacc accattatca ttcaccaccg
     4561 tccattccac ttttcctgac ggagaaacct tctgcttaag tctgaaagtg acagaagaaa
     4621 acggtgcggc cattcccccc tgagtactgc tgactttctg agctccctgt ccgacactga
     4681 cagtactgaa ggtaataaaa taaggcgtgg gattactggc ctgaagggta tcaccgcctg
     4741 taagctgcca tgtcactttc ttatatgcat catcaggaga aaaaggcaga ccatcaggac
     4801 ggaaaaaaaa cttaatccgg ctgcgtacgg caaactgaag gtaattatat ccctgtgctt
     4861 tttcagattt tccggcaaaa gaaggtttgg ccggaatatc aagaacattc agccagaaca
     4921 gtgattccct gtctgccgga agtttttctc ccgtatacat aattcgcatt gtctgtccgg
     4981 aaagcggctc catccggaaa acaggaggcg taataataaa cggcaccctg acagagtcag
     5041 gaggggaaga cgggtcccct gtatcaagcc atgcctgtat cagggatggc gtactgtctc
     5101 cgttattcag ccggacatta atactttttt gttctgcagg ataaataacc ctggtcccca
     5161 taataaccac actgccatat gagggcagag acaaaagaac ggtaaaaatg aacagcagaa
     5221 tatgtttcat acaggctccg taaacacggg gcaaaatgcc ccgtgaacac ttaagaaggc
     5281 aggtattaag ataaaaatta ctgataagcg atggtgtaat tcacagtggc agaaacgttg
     5341 ccggcggtgg cctgacccgt tgcatagtac cgtgcgttat aacgtaacgt cacatttgta
     5401 ccattaatag caacaggtgt taccgcctga ccagcagaat cagtaccgag taatatcggt
     5461 gtggtaccat cggcattcag aagctggatc tgaacatttg ttgcattacc agagccgctt
     5521 gcaatattaa ggttatgact atcataatca gtggtcgcat taggctcaaa ataggccttc
     5581 acattctcag cacctgctga agcaggactg gtacagcctt ccagtgaaat tgtgaaaggg
     5641 gtaaggcctg ccacctcccc atttgaagtt aatgaattag aggagacagt tggtagcgta
     5701 actgtcaaat tcttgctttc tgtcgtaaca gaacaagttt gatcaacaac ttttccatta
     5761 aaagtaattg taccgtcata agcaagcgta gcagaagatg acgcagccgc cagtattcca
     5821 aggataaatt gaattttccg catagaatcc tcagtgaaac tgataagaat ata
//
LOCUS       DAPHDX010000206         5779 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPHDX010000206 DAPHDX010000000
VERSION     DAPHDX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.209"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..1456
                     /locus_tag="RH058_004235"
     CDS             176..1456
                     /locus_tag="RH058_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDV3139732.1"
                     /translation="MEIRVNRILPLYLLLSVHSALASPLSTLLKDALLHDPSVMAARA
                     ESASARSRVEQARSAHWPVVTATGSKLLSQSHRYSYDYDSEDIFPGIRGEMNIFASGA
                     IEADVRRSESEAEYYYFKEKETGEETIRSFVSLYLDALREKQSIAVLEQSLSRHDAIL
                     NDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLKTTLGRLSTWTTNPVTESDL
                     ENPFSRMTESRLLTDYTQAPQKGNPSWLASSADVESKKAALKAQERARYPRVDLTGSV
                     TRDDRQIGISLSWDLFNRNASYGVSEKAAQIAAATGRLDSVARMIDETGRLSLITVRQ
                     SRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNAENELYSVGLSRVQTEDQML
                     HGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1461..3608
                     /locus_tag="RH058_004236"
     CDS             1461..3608
                     /locus_tag="RH058_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDV3139733.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPCGLDMQIGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTENIVLKGLNHWSRGRTMVVVTHRLQVLNIVDRIIV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3669..4916
                     /locus_tag="RH058_004237"
     CDS             3669..4916
                     /locus_tag="RH058_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDV3139734.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDDVPADLRERESTV
                     YRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKKLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5205..5669
                     /locus_tag="RH058_004238"
                     /pseudo
     CDS             5205..5669
                     /locus_tag="RH058_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1483 a         1191 c         1619 g         1486 t
ORIGIN      
        1 atttgacaag cttatattcc atcttattaa gactatcttt attcaatgaa ttctatttct
       61 gttcgaggat cagaatggcg gtagcgtagc tgaattcctg tgatgttatg ttgtctattt
      121 tcctcaattt acctcccggt tacatcgtta tgtctgatta tattgactgt tacttatgga
      181 gattcgggtg aacaggatat tacctttata tcttctgctt tctgttcata gcgctctggc
      241 ttctccattg tctactttac tgaaggatgc tcttcttcat gatccatctg tcatggccgc
      301 cagggctgag tctgcctctg cacgttccag agtggaacag gctcgttctg cacactggcc
      361 ggttgtcacg gcaacgggaa gtaaactcct ttcacagagt caccgttatt cctacgatta
      421 cgactctgaa gatattttcc ccggtattcg tggtgaaatg aatatatttg cttctggagc
      481 tattgaggct gatgtgcgtc ggagtgaatc agaagctgaa tattattatt ttaaagagaa
      541 ggaaacaggg gaagaaacaa ttcgctcttt tgtttcattg tatcttgatg cgctaaggga
      601 aaaacaatcc attgcggtgc ttgaacagag tctctcacgg catgacgcaa ttcttaatga
      661 tcttaatact atcagtattc atgataccgg gcgggagtct gaatttgttc aggccgaagc
      721 aagaaggttg atggtcaggc agcagataaa tacccgcacc agagtgctca aaaccacgct
      781 gggaagactt tcgacctgga ctacaaatcc ggtaaccgaa tccgatcttg aaaatccttt
      841 ttcccggatg acagagtcca gattgttaac tgattatacg caggcgcctc agaaagggaa
      901 tccgtcgtgg cttgccagca gtgctgatgt tgagagtaaa aaagcggcgc tgaaagcgca
      961 ggagcgtgcc cggtatcccc gggtggattt aacgggatct gtaacccggg atgacaggca
     1021 gataggtatc agtctgtcct gggacctgtt taaccgtaat gccagttatg gtgttagtga
     1081 aaaagctgcg cagatagctg cagcgacggg gcgactggac tcagtcgccc gaatgattga
     1141 tgaaaccgga cgattatctc tgataacggt cagacaaagt cggatggaaa tggaaacact
     1201 cagacgtcag gagcaggcct ctgccagggt tgtggacttt tatcgtcttc agttccgggt
     1261 ggcaagaaaa acactgattg aattactcaa tgcagaaaac gaactgtaca gcgtcggact
     1321 ctcccgggtt cagacggagg atcagatgct ccacggaatg ctggattatc tgtattccca
     1381 gggaatgctc ctgaaatgga gcggagtgaa tctttctggt gcagaagaaa aagtaacagg
     1441 gcagaaggac agataaataa gtgaagagca tttttgaagg attatcacag gtaacggcat
     1501 tactgggtac acctgtatct gctgagacgc tggctgccgg tactgtccgt acagatgtct
     1561 ccgggattga ttttcgttct gttggcgagt ttcttcgcag tgaagggttt gataatcatc
     1621 tttcccggcg ggctccggaa gatattcctt cacttgcggt tcctgtgctt cttttactga
     1681 atgcacagga agccattgtg gtggtgcgta ttgagggtgc cggactggcc cggcagtatc
     1741 atgtactcca gcccggggga ctccgtaaaa cactttctca cgatgaatta atggctgttt
     1801 attcaggtta ctgctggttt gttcagccac aggcaacaca ggatcgtcgt tcggagctgc
     1861 cggagtatga catgaccggg gcatggttct ggcgggtact gtggcggttc aggccatatt
     1921 acggacaggt cattcttgcg agttttatca ttaactttct tgccctggtc agttccctgt
     1981 atgtcatgaa cgtttatgac cgggtcatac ccaatcgctc ctgggaaacg ttatggatgc
     2041 tgagtatcgg ggtgattgca gccattctgt ttgagtgtgc tgcaaagttg atccggagct
     2101 atctgacgga tattgcgggt aagaaggcag acctgattat cagctcagca ctgttacggc
     2161 gggtgatggc agtgaaaatg tcggacaggc cggcgtcttc cggttcatac gccagcaatt
     2221 tgcgggaata tgagtctgtc agggagttta tgaccagcgc cacgctgctg gcttttgtgg
     2281 atttgccgtt tatcctgctt ttcatcggtg tcatcgggct tgtggggggc tggcttgctg
     2341 ttgttcctct tacactggtt ccgctggtga ttctggcagg aatattactg cagcgaccgc
     2401 tttcccgtca tattaatgcc tccatgcggg aatcatcgca gcgccaggga ttacttgtgg
     2461 aggctctgga cggaatagaa accctgaagc tgaataatgc agttaactgg gtccagcgcc
     2521 gttgggatgg atatacagcc agtgtcgccc gctcgtccat acgggtaaaa aataccagta
     2581 atgtcataat ctgcttttcc acagcagttc agcaactgaa cactgtgctg ctggtcattc
     2641 tgggaacgta ccttattcac agtgatgacc cacacaaccg aatcaccatg ggggcactga
     2701 ttgccgcagt tattctttcc ggtcgtgcgc tggcaccgct aggtcagata gcatcactgg
     2761 ccacccgttt tcagcaggcc cgcctggcca tgcagggagt gaatgacatt gtccgccgtc
     2821 cggtggagcg ggaaagtcag acacattatc tgacagtgaa ggatatccag ggagagtttc
     2881 gtttcagtga tgtctccttt tcttataagc ccggattacc cgacgctttg tgcggtctgt
     2941 cactctgtat ccggcccgga gagcgggtgg gtattattgg ctcaataggt tgtggtaaat
     3001 cgacgctgct gaaaatgatg gtggggctgt accgtcagaa cagggggcaa attactcttg
     3061 acggggttgc gatgcagcaa ctggacccct gttttgtccg ggataaagtg tttcttctgg
     3121 aacagaatcc ccggcttttt ctgggaactc tgcgtgaaaa tctgcagctt tcgcgtcttc
     3181 cgggaagcct gtcggatgtg gatatgatta atgcagccgg acgtttcggg tttgaccgct
     3241 ttattcgtca tcacccatgc gggcttgata tgcagatagg ggaaggaggt caggggttgt
     3301 caggaggaca gcgtcagatg gtcgctctga cgagaatggt actgggttct ccccgggtag
     3361 ttctgcttga tgaacccacg acgggtcttg atctgcagac agagaacatt gttctgaaag
     3421 ggctgaatca ctggagccgt ggacgcacaa tggtggtggt gacgcatcgt ctgcaggtgc
     3481 tgaatattgt tgaccgtatt atcgtgatga atgaaggccg ggtaatgatg gatggtcccc
     3541 gcgatgaaat actgaaaaaa ctgtcaggga aacagcagag tggggagagc agagcgataa
     3601 cttcatgagc gtcaaccgga taagaaataa agagcatata tgtttaacag tttgtggacc
     3661 gcaggggtat gacagaaaaa acggaacgga ataaagcgtc gtcagaggtg gtcaggcagt
     3721 cagacctggc cctgttgaca gatgtacagg cagcccttca gcaggaaaaa catcatgccc
     3781 ttttcctgat ggtgattttt ctgctggtac tggtagtgac gtttgttatc tgggcatgga
     3841 acagtcctct ggatgaagtg acccgtggtc aggggagtat cattcccggc agcagggagc
     3901 aggttataca gacccttgac ccgggtattt tgaaaacact tgaggtcagg gaaggagata
     3961 ttgttgagaa aggtcaggta ctgctgacac tggatgatac ccgaagttct gccatgctga
     4021 gggaaagtga agcccgggtg aataacctgg aagcagtcag ggcaagactc cgtgcagaag
     4081 cttattccga atcactgaca tttccggatg acgtgcctgc agatttgagg gagagggaaa
     4141 gtactgttta tcgactacga aagactgaac tggcacaaag catcgccggc cttaagcaga
     4201 gtaaggctct gctggataag gaaatagcaa tgacccggcc aattgtgcgt gaaggggcaa
     4261 tgtcggaggt cgaactgcta cgtatgcagc gtcagtcagc ggaactgcag ttacagatgg
     4321 atgagaaaca gaataaatac ctgacggaag ccggtgctga actggtgaag acagaagcag
     4381 agcttgctca ggcaaaagag aatatggctg gcagagcaga cccggttgag cgttcacgtc
     4441 tcagggcacc cctgagaggt atcgtaaaaa atatacgggt aaacactctg ggaggggtgg
     4501 tttctgcagg acaggacata atggaaatta ttccgctgga agaccagttg ctgattgaag
     4561 cttatataaa tccacgggat gttgcctatg tcagaacagg gatgccggca cttgtaaaac
     4621 tgacggctta tgactatgcc atttatggtg gtcttgacgg tgtggtgaca ctggtaagtc
     4681 cggatacttt acgtgaccag aaacgtccgg gagacttaaa actggacccg aatgaagctt
     4741 attaccgggt actggttaca accagtaata attatctgac agacaggaat gggaaaaaat
     4801 taccggttat tcctggcatg atagccagcg tggatataaa aaccgggcgt aaaacggtat
     4861 ttcagtatct gattaaaccg ataaccagaa tgaagcaggc acttcaggaa agataataaa
     4921 aaggtcttcc gggaaaaata ttttaaatca attttaaacg ggcagaatga tttcacttta
     4981 ttctgcccgg gtcattgcgc atgcctgtat gtaatgtgtg gctgaagata atacaagggt
     5041 tatatctgtc aggggaaaat aaatcctaaa aaagcagaat gattaaggag ggccagccag
     5101 agaaaataaa ttatctgtaa tctgatttgt ttttatttaa tgactccctt tagtgcggat
     5161 aaaacataag agcatacttg ttttattaac ctgaggatac ttatatgaaa atgattttca
     5221 ccggcaaggt cagtggagaa aaaacagtac tggcagccgg agcccgtcat actgtaaagg
     5281 cacaggccgg ggaacagtat ggactggtcg atgaggtgac aggtctggtt ccggatggtg
     5341 tggaagcgga ccgtacaggc gatgatttaa ttctgcgtaa aaaggaagat gatacagaaa
     5401 tccggattga gggattctgg gaaaaatgcc agcctggtga aacacagtgt acagccgtgt
     5461 ttaatgttgt aggtgaaaat ggtcaggtta ctgaagctgt cttgacccag gatggtcctg
     5521 tactggagaa ttttactgca ggacaaagtg gcacactggc tgaaggtatc agtaacggga
     5581 ttatctgggg cgggattgca tttggtgcgg gactggcggc tattgctctg cttgctggag
     5641 gtggtggcgg aagcagtcgc cataaaagtg ctgctggaga ggggcagaat caggtacagg
     5701 ctgaaggcag tcaggaaact gggaaaattg atggacctcc gacagtctct acgaaaccgg
     5761 taaatgggaa ttctttcgg
//
LOCUS       DAPHDX010000207         5755 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPHDX010000207 DAPHDX010000000
VERSION     DAPHDX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.210"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..472)
                     /locus_tag="RH058_004239"
     CDS             complement(<1..472)
                     /locus_tag="RH058_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV3139735.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQD"
     gene            complement(399..870)
                     /locus_tag="RH058_004240"
                     /pseudo
     CDS             complement(399..870)
                     /locus_tag="RH058_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(870..1535)
                     /gene="tagO"
                     /locus_tag="RH058_004241"
                     /pseudo
     CDS             complement(870..1535)
                     /gene="tagO"
                     /locus_tag="RH058_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
     gene            complement(1532..2083)
                     /locus_tag="RH058_004242"
                     /pseudo
     CDS             complement(1532..2083)
                     /locus_tag="RH058_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(2078..2824)
                     /locus_tag="RH058_004243"
                     /pseudo
     CDS             complement(2078..2824)
                     /locus_tag="RH058_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(2828..3241)
                     /gene="tssE"
                     /locus_tag="RH058_004244"
     CDS             complement(2828..3241)
                     /gene="tssE"
                     /locus_tag="RH058_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV3139736.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            complement(3248..4723)
                     /gene="tssC"
                     /locus_tag="RH058_004245"
     CDS             complement(3248..4723)
                     /gene="tssC"
                     /locus_tag="RH058_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV3139737.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            complement(4774..4998)
                     /locus_tag="RH058_004246"
     CDS             complement(4774..4998)
                     /locus_tag="RH058_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139738.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            complement(5033..5533)
                     /gene="tssB"
                     /locus_tag="RH058_004247"
     CDS             complement(5033..5533)
                     /gene="tssB"
                     /locus_tag="RH058_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV3139739.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
BASE COUNT         1299 a         1541 c         1459 g         1456 t
ORIGIN      
        1 gatcctgcca tttactcatt gcattcgtac cggctaacgt atgctcccag gaaatatctt
       61 cataaataaa ggcaattctc tcctgtggtt ccagttcttt ttccaggaca gcatgcggtg
      121 tgacaaaatc aatacttgat attcgggctt ttgacagatt tattttataa taaggctggt
      181 aaatacccgc tttactggtt cggtagagta taaattcaca tattgccatt ttttcattgt
      241 cactaagtgc ttttgccagc aatggtgagg atttatcaac cggtttggtg ataattaatt
      301 catgatgatt gacattttga cttcttgaga tggaatgcat taacgcataa accatgatct
      361 gatctttatg ttctggctgc gctttgtttc cgacagaatc aagcgttgag caacctgctg
      421 aaatcagccc ttgtgtctct ccactcaaag ataaataact tatatttgcc atgataatct
      481 cctttaatta aataattatt tttcggccgt ggtaccggtt gccgcctctg gcatgacgtt
      541 attcttcagc agcagccagc ggcttaacac gccttcgagt cgctcattaa tttgtttttc
      601 cagcgcatct gtattctgct tctgcgcacg ggcatcctgg tactgcgtca acagagactc
      661 aacgggtgtt tcctgcccca gtccccgctc cgcctgccag atgacgtttt tgatatagga
      721 aagggttagt ccctctttct cgcgctgcat cactagatct gcaaaagcat gtaagtcctg
      781 gcgggtctgc aaccagccac gcaactgcgg gctactctgt gcgcgggttt tgagtgcctc
      841 attccattgt gtcgaggcct gttgctgttt ccggcggcat tgtctgcgcc ggtatccacc
      901 gtcagcgttt ttgcgccgaa cagttgctta atttcatcaa tcccggataa cccccggctg
      961 gactccagca gggtgccgtt ctcgcgcaca aaccagtggc tgcgcaatgc ccggttgtcg
     1021 gcattcagag tgacggcgat atcatggaca tccagagggt gcatcagcgc cacctgcatc
     1081 cgggtgatat tgtccacgca actgaacatc agcaccgggc gtggcggaac atgaccgata
     1141 gccggggtgg ttatcaccac cgtcgggcgc tcccccggca cctgcgtcac cagcagttct
     1201 gtggtgttgc cctcgcggcg tttttcctgc tctgtggcgc gggtccaggc ctcgcccaca
     1261 aaaccggctt tgaccagcgc cccgtcaaac cctgacgggg acagcggtgc cagcaggcgg
     1321 tcatagcagt caagccgctc cagcgccgcg gactcccgcc gacaggactg catggcctgt
     1381 aacgtggctg tcgcatccgg cggggtcgtg gtggcgacgg cgaccgccac accggccatt
     1441 gacgatgctt ctgcgggcgc ggcagtggcg ggtaagcccg tcatcaggcc cgcgataaac
     1501 catcctgtct gccggaaaac gcgcaacctg ctcataaaac cggttcatcc tctgtgacgg
     1561 actcgtcgtc cagagcgtct tccacatctg ccgtaaatgc gccgtccact gccgacaacc
     1621 gaatccgggc aatcgccgtg ttagccgcca ttttctgcaa cagcagcagc gagagcggcg
     1681 gtagcatgtc gccatcgatg accgattcca gcatcctcgc gccgttttcc gcgcgggtga
     1741 cgcggctcat gatttcgtcc gtcacttccg gttcaatgac cacttctgca ccaaagcgac
     1801 tgcgcagcac gttatccaga cgggccagtt ttccggcgat aatggtggcg agcgtctctt
     1861 tcgacagcgg cagatacggc accacttcca tacgcgccag cagggcaggt ttgaagaagt
     1921 cggccagcac cggatacagt acttcctgca tggtttccgg gtcatcggca tgctcgacta
     1981 ttacctggta gccgaggttg gacgtcagga agaagacgat atttttgcag tcaatcaggc
     2041 ggccttcacc gtctgccatt tcgcccttat cgaacgcctg gtagccgctg tggacgtttt
     2101 tcggccaggg cagcatggca tcctgcggct caaaaccgac agcttttagc ataaactcag
     2161 gcaggcgaat atgctgttca cccacagtca ggtcggcacc ctgcaagtat tcgcagaacc
     2221 ataaataaag ctggtcacgg gtatagttgt cgtcattgcc gagccagaag cgaagtttgt
     2281 tcagatccag cgtgcggaag tccgttcccg gtgcgaccga aaaggtgatg ttgataacac
     2341 cattacgggt cgtactgcgg ttttcaatct gctccaggcg aacgggcagc agccagatat
     2401 cccggcagag ggtgaaggta cagggaggcg gctcctcctt tttagcatca gagggcagca
     2461 cttcatcaac tctgatttcc ccggcgttgg ttgtaaactg ctcattacgg gggattaaca
     2521 ccggtacaga agacttatcc atatccggcg tatattcaat aagggtcatt gccggaaccg
     2581 gacgcaggta attaggccat agcatcttaa taagcccgtg cgtcagttct gggaattcat
     2641 cctcaatttt ctggcggaga ttgccggtaa gaaaagccac cccttccagc aggcgttcaa
     2701 tatccggatc cgcatctttt tcggccagga agcgggccag atggggtttt tccgttgcca
     2761 gcagcttgct aagctggcgc aggtaatcga gttcttcccg gaaatagcgt tcttcaaatt
     2821 ccatttgtca gtccacccgg taatgctggc tgccattcgg tgccatatca aattcaagca
     2881 cctcatgcgt ttcgttaaat gtcacccagg ctgtaatatg aaacgtcata tccagcgggc
     2941 tggaggcgga tgaaaccact gcctgcactt cagcgtgtga gatccggggt tcatagcgtt
     3001 ctatacattg ctcgatggcc cgactcatgg attcgcggaa gttttcagcc ccttccggtt
     3061 catcaatgcc cagctcaggc gcaccccggc aactaccgga ccgggtatta agaatgtttc
     3121 gtaggttctg gcgaatggat tgcctgagcg aatgaatact gcctccctgc cggtttctgg
     3181 ccgaagaggc ttccatacgc tcaaaaagag aacctgtggt tctttcccga tccggtatca
     3241 tggtgtgtta ctccctgtct aaccggccaa ccagggaaag ctcaaaattt gcccccataa
     3301 atttaaaatg aggtctcacg cttagcgcaa cctggtacca gccgggttcg ccttctacat
     3361 ccataacctc aactttggca gcgcgcagag gtttgcggct gcgcacgtct gcaggcgggt
     3421 tttcctggtc ggcaacatac tgacggatcc aggtattaag ctctctttct aagtcgctcc
     3481 tttctttcca tgaccccagt tgttcacgct gcaacacctt gatgtagtgc gctaaccgat
     3541 tgatgatgaa gagataagga agctgcgtac ccagcttata gttggtttct gcatcttttc
     3601 ccggaaagtg cttcggtttt tgtaccgagt ttgcagagaa aaaggctgca ttatcagagt
     3661 ctttacgcat ggtaagggtg ataaaaccct cttcagccag ttcaaattcg cggcgatcgg
     3721 tgaccagcac ctcggttggg attttggcct gaatctgccc catcgtttcg aacagatgca
     3781 ccggcagatc tttcactgcg ccgccgcttt gcggaccgat aatgtttgga caccagcggt
     3841 atttggcaaa actgtctgcg atattgcatg ccagcatcca ggccgtatta ccccacagat
     3901 aatcttcgtg gttctggctg acatcttcgt aatagttaaa gttcttaaca gggttatctg
     3961 ttggtgaata gggctggcga agcaggaagc ggggtgccgt aagccctaaa tagcgcgaat
     4021 cttccgagtc acgcagggcg cgccatttgg tgtaggccgg accttcaaag atggcataaa
     4081 gatctttgat attaggcagt tcggtccacg agttcagccc cataaactcc ggggaaacgg
     4141 aggacaggaa cggtgcgtgc gccattgcgc ccacggcact gacgtattgc agcagtttca
     4201 tgtcaggcgc ggtatttttg aactcataag cgcccagcac ggcagcgatg ggttcaccgc
     4261 caaactgtcc gaaaccggac gaataaacgt gcttataaaa tccggactga ataatttctg
     4321 gcgcaaactc aaagtcttcc agaagttcct gcttggtgac atgaagcacg ttgaccttga
     4381 tgttttcacg gaaatcaacg cgatccacca tggttttcag tgagcgccag aacgactcga
     4441 cctgctgaaa ggcaggcgca tggatgatga catccatctg gcggctgatg cgttcatcaa
     4501 tatctgcaat catgctgtcc acggccagtt tattgaccgg ttcggcagat gcagtggatt
     4561 gcaggatgct ggcaataaag gctgtcacac cctggcgggc aacgtcataa ccttccgaac
     4621 cgggctgaat acgggtctgt gccatgattt gatcaagcag cgacgatgcc tgctcaggct
     4681 gcccggcatg actggaaacg agttcctctt cctgtaaaga catactgaaa atccttctgt
     4741 tttagctgtg tttctgaaac cggcttttcg ccgtcagtga agtacgcgct gccagtgcga
     4801 cgcgctctgc tgaaactcac aaaaatggat aatctcatcc aatagatcac tgatacggcg
     4861 atccagtgtt gtaatcagcg cagccacacc atcctggttg atttcaacgg acgggctgaa
     4921 acatacctgg taaaccacct gcagaatgtc tgcaacagag aggtggtatg tcccggagat
     4981 atttttgtgt gccgtcatgc ctggataacc ctgtttaatg aaatagtggt ggttatttat
     5041 tgctcacctg gccgatttct ttgagcaact gctcgcggga ctcttcattt tccagcaatg
     5101 cctggagctg ggtacggaat gccggaaggt tacccatcgg ccccttcagt gcagtcagtg
     5161 cttcacgcag ttccagcaat ttattcactt ccggaacctg ccgggccacg ctatctggtg
     5221 aaaaatcatc cagtgacttg atattcagcg ccacattcag ctcatcgtcc gttgtgcttc
     5281 ccttcagcgt ggcggggacg gtaaaattga ggccaatggc tgcttcagcc atcactgcac
     5341 cgaaattgtg cttgttaacg gaaactgcct gacgttcatc cagtggggat gtttcctgcg
     5401 tatttccggt atcgccgaca acgagcatgt taagcggaag ttccacttca gccgtctggc
     5461 catctgtttt tgggacatac gatatattaa tacgctcgcg aggcgcgacg ctgccttcaa
     5521 attttttgct cataaaagtg atccgtcatg ttatctcgta caggccatga acacggcata
     5581 tttgtaccgc aaaaaaatat cggcaatgcc gccctaatgg cggtgaaaat aaaatcacca
     5641 ctccttcatc accccgcatt ctacgcttca aaataaataa gaaaaggact ttttatcgat
     5701 cgtaaagagg ggtattctgc aagcgttaaa tagattgtgt gaacctgtgc aattc
//
LOCUS       DAPHDX010000208         5730 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPHDX010000208 DAPHDX010000000
VERSION     DAPHDX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.211"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            149..799
                     /gene="csgD"
                     /locus_tag="RH058_004248"
     CDS             149..799
                     /gene="csgD"
                     /locus_tag="RH058_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV3139740.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            804..1193
                     /gene="csgE"
                     /locus_tag="RH058_004249"
     CDS             804..1193
                     /gene="csgE"
                     /locus_tag="RH058_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV3139741.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1218..1634
                     /gene="csgF"
                     /locus_tag="RH058_004250"
     CDS             1218..1634
                     /gene="csgF"
                     /locus_tag="RH058_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV3139742.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1661..2494
                     /gene="csgG"
                     /locus_tag="RH058_004251"
     CDS             1661..2494
                     /gene="csgG"
                     /locus_tag="RH058_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV3139743.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PSGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2558..3049)
                     /locus_tag="RH058_004252"
     CDS             complement(2558..3049)
                     /locus_tag="RH058_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV3139744.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3151..3705)
                     /gene="ycdY"
                     /locus_tag="RH058_004253"
     CDS             complement(3151..3705)
                     /gene="ycdY"
                     /locus_tag="RH058_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV3139745.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3729..4466)
                     /gene="ycdX"
                     /locus_tag="RH058_004254"
     CDS             complement(3729..4466)
                     /gene="ycdX"
                     /locus_tag="RH058_004254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV3139746.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4521..5459)
                     /gene="ghrA"
                     /locus_tag="RH058_004255"
     CDS             complement(4521..5459)
                     /gene="ghrA"
                     /locus_tag="RH058_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV3139747.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1555 a         1413 c         1321 g         1441 t
ORIGIN      
        1 cagatgtaat ccattagttt tatattttac ccatttaggg ctgatttatt actacacaca
       61 gcagtgcaac atctgtcagt acttctggtg cttctatttt agaggcagct gtcaggtgtg
      121 cgatcaataa aaaaagcggg gtttcatcat gtttaatgaa gtccatagta ttcatggtca
      181 tacattattg ttgatcacta aaccgtcttt gcaagcgaca gctctcttgc agcaccttaa
      241 acaatcgctg gcaattacag gaaaattaca taatattcaa cgttctctgg acgatatatc
      301 ttcaggctct attattcttc tggatatgat ggaagcagat aaaaagctta tccattattg
      361 gcaggatact ttgagcagga aaaacaacaa tatcaaaata ttgttgctaa atacgcctga
      421 agattacccg taccgcgaca ttgaaaactg gcctcatatc aacggcgttt tttatgccat
      481 ggaggatcaa gaacgtgttg tcaatgggtt gcaaggcgtc ctgcgcggcg aatgctactt
      541 tacgcaaaag cttgccagtt acctgattac acattctggt aactatcgtt ataacagcac
      601 ggaatcagcc ctccttactc atcgggaaaa agagatcctg aataagctgc gtatcggcgc
      661 gtctaataac gagatcgctc gttcgttgtt catcagcgaa aatacggtta aaacgcatct
      721 ttataatctt ttcaagaaga tagccgtaaa aaaccggaca caagcggttt cctgggcaaa
      781 cgataacctc aggcgataaa gccatgaaac gttatttacg ctggattgtg gcggcagaat
      841 ttctgttcgc cgcagggaat cttcacgccg ttgaggtaga agtcccggga ttgttaactg
      901 accatactgt ttcatctatt ggccatgatt tttaccgagc ctttagtgat aaatgggaaa
      961 gtgactatac gggtaactta acgattaatg aaaggcccag tgcacgatgg ggaagctgga
     1021 tcactataac ggtcaatcag gacgttattt tccagacttt tttatttccg ttgaaaagag
     1081 acttcgagaa aactgtcgtc tttgcactga ttcaaactga agaagcacta aatcgtcgcc
     1141 agataaatca ggcgttatta agtacgggcg atttggcgca tgatgaattc taaataaaaa
     1201 attgttcgga ggctgcaatg cgtgtcaaac atgcagtagt tctactcatg cttatttcgc
     1261 cattaagttg ggctggaacc atgactttcc agttccgtaa tccaaacttt ggtggtaacc
     1321 caaataatgg cgctttttta ttaaatagcg ctcaggccca aaactcttat aaagatccga
     1381 gctataacga tgactttggt attgaaacac cctcagcgtt agataacttt actcaggcca
     1441 tccagtcaca aattttaggt gggctactgt cgaatattaa taccggtaaa ccgggccgca
     1501 tggtgaccaa cgattatatt gttgatattg ctaaccgcga tggtcaattg cagttgaacg
     1561 tgacagatcg taaaaccgga caaacctcga ccatccaggt ttcgggttta caaaataact
     1621 caaccgattt ttaagcccca gcttcataag gaaaataatc atgcagcgct tatttctttt
     1681 ggttgccgtc atgttactga gcggatgctt aaccgccccg cctaaagaag ccgccagacc
     1741 gacattaatg cctcgagctc agagctacaa agatttgacc catctgccag cgccgtcggg
     1801 taagatcttt gtttcggtat acaacattca ggacgaaacc gggcaattta aaccctaccc
     1861 ggcaagtaac ttctccactg ctgttccgca aagcgccacg gcaatgctgg tcacggcatt
     1921 gaaagattct cgctggttta taccgctgga gcgccagggc ttacaaaacc tgcttaacga
     1981 gcgcaagatt attcgtgcgg cacaagaaaa cggcacggtt gccattaata accgaatccc
     2041 gctgcaatct ttaacggcgg caaatattat ggttgaaggt tcgattatcg gttatgaaag
     2101 caacgttaaa tcgggcggtg ttggggcaag atattttggg atcggtgcag acacgcaata
     2161 ccagctcgat cagattgccg tgaacctgcg cgtcgtcaat gtgagtaccg gtgagatcct
     2221 ctcctcggtg aacaccagta agacgatact ttcctatgaa gttcaggccg gggttttccg
     2281 ctttattgac taccagcgct tgctggaagg ggaagtgggt tacacctcaa acgaacccgt
     2341 tatgctgtgt ctgatgtcgg ctatcgaaac aggggtcatt ttcctgatta atgatggtat
     2401 cgaccgtggt ctgtgggatt tgcagaacaa agcagaacgg cagaacgaca ttctggtgaa
     2461 ataccgccat atgtcggttc caccggaatc ctgacagata aaaaagccgg aattatcatc
     2521 cggcatttta ctgtttaagc gaaagcgtga gtgcccctca cgctttattt ttgatttcct
     2581 gctcacggtg agccgtcttt gcactacgcg ccgccagcca caggccgctg tttttcattg
     2641 cgtaaccaaa taccagccca agcgccagcg aaggtaacac cagtttccag tcaccctgcc
     2701 cggcaaacgt cgcgcatgcg cctataaacg tccccggaac aaatgaaagc agcagctgtt
     2761 tggcctgaat acacatcaga aacgcgacaa taccggttat gacataaccg agaatttcca
     2821 gatgtggtgc cagtgcacta ccgtaaataa tgaccatcgc ccacaccacg ccactaagca
     2881 gggttgcagc ggagatcgcc agccctttca gcccaccttg cgggcaggca aagtaggccg
     2941 tacagcccag gaagcccgcc cagcttagta agcccaggga aacagccacc catccccaga
     3001 taccggagag aatgcctgtt gtgattgcaa tagagagaag tatgttcatg ccgcgcatga
     3061 tagcagaaaa cgcggccatg aatgagatcg agcacacatt ttatgcaaca acaaattatc
     3121 tgttgcagtt atgagtgacg atgatcacat ttactcttca gaatcttctt ccagctcgtc
     3181 ccacattgca ctaatggcat cacgggttag cggtgccatg gtgcgccaga aaggcgtggt
     3241 tgcatgggcc tccactttgc cgaggaacgc accacaccag ggtaacagat actcactgaa
     3301 cagtgtttcc agtgcttcgc tctcatcttc cgttgactga tcttccagcc aggaagccgc
     3361 aagcagcaat gtgccgatgt gatcggctgg cgtatccgcc agtggcatcc ctcgttcgga
     3421 aagaaaagcg cgcacttccg cttccgttgc gtcctcaacc catgcgctac gatatggcgg
     3481 cacggcacat tcatcgccga taaacaacgc attgtaatcg gcagagactt gcgccatatc
     3541 acagcttttc tgtaaacgcg tcagtaactc atcctgctcc agtggccagt tcgcagccag
     3601 tttcccctca cgaatcaggg taaacagcgg caccagtaaa ggatcttgcg gttggcggta
     3661 atagagcgaa cccagcacac gacagaggat agaaaactcg ttcattaata tatttccatt
     3721 actagtaatt aaaggtctgc aaattccgca atcggtgcca taccgcgaga ttcaaggaag
     3781 ttcagtaagc ggcgcggcga aacattcaaa atgcgctctg gcggaaaatc taccgcgtcg
     3841 aggattttaa gacactcttc aaattccccc atggtaaacg cggtgtgaga atccgagcct
     3901 aatgccaccc aacctccagc atcacgtacc gctgcggcta ccgcacgaca gttgtcttca
     3961 ctgcccttac gtgagtgtaa aaatgaagaa ttattgattt ccagcgccac ctgatgtttc
     4021 gcggctgctt cagcaacggc tttcacatct atttcatatt tgggatttcc gggatggctt
     4081 attatatgca cattgccgct ggcgatagtg gcgatcatcg cttgtgtatt ggtcgctttg
     4141 tcatgtggtg caaaaaccgg ctcatgaaaa ccggcaataa tcagatccag cgagtcaaac
     4201 attttaccgc tgcaatcaat ttcaccatca acatttttaa tgttagcttc gatgccgcgc
     4261 aggatcccta ccccatcaac cactcgcggc cagatacgca tgttaatgaa gtgccagtga
     4321 tgcggagcat cttccatatc cgggccatga tcggtgatcg caaaaagttt aatgcccttt
     4381 tgtttggcct gggcaatgta atcactcaat gtgctatatg catgtgtgct ggcaacggta
     4441 tgcatatgaa ggtcgacggg atacataact ctctcctgta ttcaattccc gccaaggata
     4501 gcaggaatcc tgatgcttta ttagtagccg cgtgcgcggt cgacttgccc gcagaccctc
     4561 tccccttttt cgagctgggc aatggtgcga gaaatgtact ccacagcttc agcgggacgg
     4621 gtaatcgcgg cgacatgtgg tgttatcgtc acgcgtggat gttgccagag cggactttca
     4681 ggcggtaagg gttcacgatt aaaaacatcc aacattgcgc ctttaacttt gccgctatcc
     4741 agcgccgcga gcaggtcatc ttccacaaca tgaacaccac gcgccaggtt gaggagatac
     4801 gcgccatccg gtaatttttc gagtaattgt tgattaataa tgccgacggt ttcaggggta
     4861 ttcggtaaca aattaatcaa tacccgacat tggctcagaa atgcagacag ttcttcccgt
     4921 ccggcaaagc tttgcacgcc aggccacgat ttacgggttc gactccagca acgcaacgga
     4981 aagcgccagg tttgcagact ctgagcaact ttactgccca gtacgcctgc gcccaaaatg
     5041 ccgatggtaa aatcttcccg atgatattca ggcagcggtt gccaatgcga actattttgc
     5101 tggatgcgat aatcgtcaaa acgtcgaaac caatgcagca cctgactgac agcatattcc
     5161 tgcatttgct cgcccatacc ggtatcttcc aggcgaaaaa gtggaacaga agggttcagc
     5221 atttcagggt gtgcctgtag cttgctcaaa atagaatcaa caccggcccc gagtgcgaac
     5281 accgctttaa gatcgcgccc tgccagcatt tcaacaggag gatgccagac taaagcataa
     5341 tcagcagagt cattatctcc gcttttccat gctctgactc ttgcctgagg aatagctttg
     5401 cgcagtgcct caatccacca ttgggtatcg aacgttgggt gataaaagat gatatccata
     5461 ctgactcccg aaaagcgttg tgcgaaattt attcgcactt atcgttatga tctacaaagg
     5521 ccaccagcat aacaaatccg tggtcggtgg caaaaaaagc agatttcgct tattaaaacc
     5581 acacattgat tgaagtttga ataaacgcgc gattttttca aaaagtttgt tgacctcagg
     5641 tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat ttggtgaggt
     5701 gtccgagtgg ctgaaggagc acgcctggaa
//
LOCUS       DAPHDX010000209         5728 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPHDX010000209 DAPHDX010000000
VERSION     DAPHDX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.212"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="RH058_004256"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="RH058_004256"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV3139748.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="RH058_004257"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="RH058_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV3139749.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /locus_tag="RH058_004258"
     CDS             complement(1535..1801)
                     /locus_tag="RH058_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV3139750.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="RH058_004259"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="RH058_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV3139751.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISQIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1435 a         1469 c         1480 g         1344 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaagccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
      841 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     1081 tagtcctgag tcagaaactg taattgaaaa ccacaatgaa gaaagtagcc gcgtttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aagattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtcgcatttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttaaaga acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgacg ctgtacaaac tggcaggcga actaacaccg tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg ttatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc agcaaacgtc caggaagcgc aagactttgc
     2521 tctcatttcg caaatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga
     2641 tctcatgccg caggtcgaaa ttgatgctca tcgcgcccgg gcactcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     2941 tggcgaaaaa gttggcgtgc tgaaagttcg cctgtaccgt cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtaatgacc gcactggcag aagcctttaa
     3121 taatggcgag cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtgaccaat ctgtcactgc cgttgccgga
     3301 aaacaccctg ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt
     3661 tcaggccgtg ctaaatcaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcgcg
     3901 tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcagcga tgctcgcagg
     4021 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtct cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attactgact cagctctatg gcgaccggat gttgatcgct aacgccactg gctgttcttc
     4621 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     4681 ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacggcgt tgaaatcaga cgccacgcca gaggttcgtc gtgaacaggt
     4861 tgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tacgtgatgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcatcg aaagcgacac cgctgggtgc
     5101 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tgaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca ttgcttatag
     5281 cccgtgtgaa gagcatggtt acgatctggc actcagccac gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt tgatccgcgt cgtgccgatg aaggcaaact
     5401 gccgctggcc ttggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcggctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaaagccc
     5641 ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc
     5701 aacagaaaat aaaatctgtg acaataaa
//
LOCUS       DAPHDX010000210         5724 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPHDX010000210 DAPHDX010000000
VERSION     DAPHDX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.213"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            324..2756
                     /locus_tag="RH058_004260"
     CDS             324..2756
                     /locus_tag="RH058_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV3139752.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPDSDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2823..4292
                     /locus_tag="RH058_004261"
     CDS             2823..4292
                     /locus_tag="RH058_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV3139753.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4292..5473
                     /locus_tag="RH058_004262"
                     /pseudo
     CDS             4292..5473
                     /locus_tag="RH058_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         1713 a         1370 c         1367 g         1274 t
ORIGIN      
        1 attttcttcc actaatgcat aaagaagaat accaacaaag cacagagata aaacaatatc
       61 tttccacaga tatttataag ggagaagtat aaaaccaagg agtagataaa ttagcagaat
      121 gacatctaat gccttagtac aaaaatattg tttcactcct tgcaagaacc gaacattctc
      181 catatgaatc cttatcctac tattgatgaa actcaagaat acctttaaaa cagtgactta
      241 acgctgataa tactctttga acagatgaat tcatctattt ttctgtgtag aatttgcgca
      301 tcgcatttag atgagaacac cccatggcgg aactgaacct aagtaacctg acggaagcag
      361 acatcattac caaatgcgtt atgccagcca ttctcaatgc aggctgggac aacacaacgc
      421 aaatcagaca ggaggtcaaa ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg
      481 cacgcagaac ggtaaaatct gctgacatcg tgctgtatca caaaccaggc attcccttag
      541 ccgtgattga agcaaaagcc aacaaacatg aaattggcaa agggatgcaa cagggcattg
      601 aatatgcgcg cctgctggac gttccttttg tttttgccac caacggtgat ggctttatct
      661 tccgcgatgc cacggcagcc gaaggtgaat gccttgaaaa gcaaataacg ctggatgact
      721 tcccctcccc cgctgaactc tggcaaaaat tctgcctctg gaaaggatac acacaagctc
      781 agcttccggt gattactcag gattattacg acgatggcag cggtaaatcg ccacgttatt
      841 accagcttca ggcaatcaac aaaaccattg aagccgtctc caacgggcaa aaccgcgttc
      901 tgctggtcat ggcgaccgga acagggaaaa cctataccgc attccagatc atctggcgcc
      961 tgtggaaatc aaaaaataaa aaacgcattt tgttccttgc cgatcgcaac attctggtcg
     1021 accaaaccaa aaataatgat ttccagccat ttggtacggc aatgaccaaa gtcagcggac
     1081 gcaccattga tcccgcttat gaaattcacc tcgcgctcta tcaggctata actggcccgg
     1141 aggaagacca aaaagcgttt aaacaagtcg caccagattt cttcgatctg atcgtgatcg
     1201 acgaatgcca tcgcggcagc gcatccgaag acagcgcctg gcgagagatc cttgattatt
     1261 tcagttccgc cacccaaatt ggcttaaccg ccacgccaaa agagacgcat gaagtctcca
     1321 gcacggatta cttcggcgat ccggtttacg tctactcgct aaaagaaggg atcgaagacg
     1381 gcttcctcgc cccttataaa gttgtccgtg ttgatattga tgttgatctg caaggctggc
     1441 gcccaaccaa agggcaaact gacttaaacg gcgaagtgat cgacgatcgt atctataacc
     1501 agaaagattt cgatcgcacg atggtaatcg acgaacgcac agaactggtt gccagaacca
     1561 ttaccgacta cctcaagcgc accaatccga tggataaaac catcgtcttc tgtaacgaca
     1621 tcgatcatgc agaacgtatg cgccgcgccc tggttaatct caacccggag caggtgaaaa
     1681 agaacgacaa atacgtcatg aaaatcaccg gcgatgatga aattggcaaa gctcagttgg
     1741 ataacttcat caacccgaaa aaaccgtacc cggttatcgc gaccacttca gagctgatga
     1801 ccaccggtgt ggatgctaaa acctgcaaac tggtagtact ggaccagaac atccagtcga
     1861 tgaccaaatt caagcagatt atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac
     1921 tctggtttac catcctcgac tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg
     1981 atggcattcc cgaaaaagtc atggatacca caccagagga tatcgccgat ccagactctg
     2041 attttgaaga gaaactcgaa gaaatcagcg aacatgacga cgaacaggta acaggcgttg
     2101 atgaaccgcc tgcgccacca taccaggtta ccgataccga tgatgtcggc ccacttccgg
     2161 aagaagacga gaagaaaatc cgcaagtttc acgtcaacgg tgtagcagtg ggcgttattg
     2221 cgcagcgtgt tcagtattac gacgccgacg gtaaactggt taccgaatcc tttaaagatt
     2281 acacccgcaa aacactgctc aaagaatatg cctcgctgga tgactttacc cgcaagtggc
     2341 aggacgccga tcgcaaagaa gcgatcattc acgagctgga gcaacagggg atcatctggg
     2401 aagtactggc agaagaagtc ggtaaagatc tcgacccatt cgacatgctt tgccacgtag
     2461 tgtatggtca gccgccgtta acccgcaaag agcgcgccga gaacgtgcgc aagcggaact
     2521 acttcacaaa atactctgaa gcagcgcaag ccgtgctcga taatctgctg gataaatacg
     2581 ccgatgcggg cgtacaggag atcgaaagta ttcaggtgct gaaacttaag ccattcgaca
     2641 gcatgggcac cttaccggag attattaaaa ccggatttgg cgaccgtaac gggtataatc
     2701 aggcgctcag cgagctggaa aacgaaatct accaattacc gccccgctct gcttaacaac
     2761 tctcttttgc gccttctgca accatcatgt tgcagaaggc cttgtttttc atggaataca
     2821 acatgtctat cagttcagta atcaaatcat tacaagatat tatgcgcaaa gatgccggtg
     2881 tggatggcga tgcgcagcgt ctcggtcagc tctcctggct gctatttttg aaaatcttcg
     2941 atgcccagga agaggcgctg gaactggagc aggataacta tcaatatccg atcccacagc
     3001 gttatttgtg gcgcagttgg gccgccaacg cgcagggcat taccggtgat tccctgctgg
     3061 aattcgttaa tgatgatctg ttcccggcgt tgaaaaacct cactgcgcct atcgataaaa
     3121 acccacgcgg ctacgtggtg aagcaggcgt tcagcgatgc ctataactat atgaaaaacg
     3181 gtacgctact gcgtcaggtg atcaacaagc tgaacgaaat tgactttacc agcgccagcg
     3241 aacgccatct gtttggtgat atttacgaac agatccttaa agatctgcaa tctgcgggca
     3301 atgctggcga attctatact ccacgcgccg tcactcgctt tatggtggat cgcgttgatc
     3361 cgaaactcgg cgaatccatt atggacccgg cctgcggtac gggcggtttt cttgcctgcg
     3421 cgttcgatca tgtaaagaac aaatacgtga agagcgtcgc cgatcatcag acgctgcaac
     3481 aacagatcca cggtgtcgag aaaaaacagc ttccgcacct gctggcgacc accaatatgc
     3541 tgctacacgg catcgaagta ccggtacaaa ttcgtcacga caacaccctg aacaaaccgc
     3601 tttcctcctg ggatgagcaa ctggatgtca ttgttaccaa cccgccgttt ggtggaacgg
     3661 aagaagacgg tattgagaag aactttccgg cagagatgca aacccgcgaa acggcggatc
     3721 tgttcctgca actgattgtg gaagtactgg cgaaaaacgg tcgcgcggcg gtggtattgc
     3781 cggatggcac gctatttggc gaaggcgtta aaaccaaaat caaaaagcta cttaccgaag
     3841 agtgcaacct gcatactatc gtgcgtttac cgaatggggt gtttaacccc tataccggca
     3901 ttaaaaccaa cctgttgttc tttaccaaag gtcagccaac caaagagatt tggttctatg
     3961 agcatccgta tccggcgggc gtgaaaaact acagcaaaac caagccgatg aagtttgaag
     4021 agtttcaggc ggagattgac tggtggggta acgaggccga tggttttgcc agccgcgtcg
     4081 agaatgagca ggcatggaaa gtcagcattg atgacgtgat tgcgcgtaac ttcaatctgg
     4141 atattaaaaa cccacatcag gcggaaaccg tcagccatga tccggacgag ctgttagcgc
     4201 agtatgcaaa acagcaggcg gagatccaga cgctgcgtaa tcaactgcgc gatattcttg
     4261 gcgctgcgct gtctggcaag gaggttaact aatgagtgtg gaaaagctga tcgttgatca
     4321 tatggaaacc tggacctcgg cgttgcaaac ccgttccacc gccgggcgcg gcagttccgg
     4381 aaaaattgat ttatatggca ttaagaaatt acgtgagctg attctggaac tggctgtgcg
     4441 cggtaaactg gtgccgcagg acccgaacga tgaaccggcg tcggagctgc tgaagcgtat
     4501 tgcggcggaa aaagcggagc tggtgaagca aggtaaaatt aaaaagcaaa aaccgctgcc
     4561 ggaaattagc gaggaagaga agccgtttga attgccggag ggatgggagt gggtcacact
     4621 ggcaaccgta ggtgaaatag tcggaggagg aactccaaag tccgataatc cgcaattttg
     4681 ggccaaaaat ggtattaagt ggattactcc agcagacctc tacggactca aagggaagta
     4741 cattacatct ggagcccgtg atatttcacc cgcaggactg tcaaactcat cagcccggtt
     4801 gatgccaaag gggagtgtgc ttttctctag ccgagcacca attggatatg tcgctatagc
     4861 agatgcagaa ctgtcaacaa accagggttt taaatcttgt gttccatata tcaaagaaag
     4921 tgcagaatat atatattact ttctactggc atctgcaaaa aaaatagatg cagaggcaag
     4981 tgggactaca tttaaagaag tctctggggc aattgtttcg aaaatattat tacctttacc
     5041 gccattgagt gaacaactaa aaatcgtctc aagggcaaat gaacttatgt ccctatgcga
     5101 ccaactggaa cagcaatccc tgacaagtct ggacgcacat cagcaactgg ttgaaaccct
     5161 gttgggaaca cttacagaca gccaaaacgc cgaggaactg gctgaaaact ggacgcgtat
     5221 tagcgagcat ttcgacacac tatttaccac cgaagccagc gtggatgcgt taaaacagac
     5281 cattctgcaa ctggccgtaa tgggtaaact tgtgccgcag gatccgaatg acgaaccagc
     5341 ctccgaactg ctcaaacgta ttgcgcagga aaaagctcaa ctggtgaaag aagggaaaat
     5401 aaaaaaacaa aaatcgttgc caccgattag cgatgaggaa aaaccgtttg aacttccgga
     5461 agggtgggag tggagttacc tatcagatat tggcatactt gctagaggaa gatctaaaca
     5521 tagacctcgt aacgatccca ctctttatgc tgatggcaca atcccattag tacaaacagg
     5581 tgatgttgca agatctaatg gatgtataaa tacatattcc gcgctatata atcaactagg
     5641 attaagtcaa agtaaattat ggaataaagg tactctttgt attacgattg ctgcaaatat
     5701 tgcagactct ggtattttaa actt
//
LOCUS       DAPHDX010000211         5496 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPHDX010000211 DAPHDX010000000
VERSION     DAPHDX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.214"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            199..471
                     /locus_tag="RH058_004263"
     CDS             199..471
                     /locus_tag="RH058_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139754.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            452..1660
                     /locus_tag="RH058_004264"
     CDS             452..1660
                     /locus_tag="RH058_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV3139755.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(1950..2054)
                     /locus_tag="RH058_004265"
                     /pseudo
     CDS             complement(1950..2054)
                     /locus_tag="RH058_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(2056..2379)
                     /locus_tag="RH058_004266"
                     /pseudo
     CDS             complement(2056..2379)
                     /locus_tag="RH058_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3122..4099
                     /locus_tag="RH058_004267"
     CDS             3122..4099
                     /locus_tag="RH058_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV3139756.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(4378..5118)
                     /locus_tag="RH058_004268"
     CDS             complement(4378..5118)
                     /locus_tag="RH058_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3139757.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(5239..5427)
                     /locus_tag="RH058_004269"
     CDS             complement(5239..5427)
                     /locus_tag="RH058_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV3139758.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT         1347 a         1376 c         1456 g         1317 t
ORIGIN      
        1 caagataagg gaaggttcta tttattgaca ttaataccct cgacaattgc tgagcagaag
       61 cccccacgac aagtgacaga atgacaacat ctacagtggg gagcgtttta tcgatgaaga
      121 accacggcag cctggcggct gcaaatcgtg gtgtggcaga atatgagctt tcagagtaac
      181 catccagaat gagttcccat gtcacacaac ctggagcacc agaaagtcca caccagaatg
      241 gtgaaagagg ttctgaaagc cgtcgccagg gccaataatc acccttatca gtctgtattt
      301 acagacttta tcgccggtca tccctcctgt acggtctgtt tctgggaaac cttccacaaa
      361 atgtacccgg attcaccgta cgaatacgtc acgttctgcc atacctgccg tcgttttgat
      421 ttatacgaaa ctgaagcgga gatgaaggcg gatgacccga agtggtggtg attttcagcc
      481 ccgaccgctg aaacgcctgt tcacagccaa ccagtgctgg acatccttcc tggatgcggg
      541 cggcctgcgc gatatcgagg ttgaggctgt cacaaaaatg ctggcctgcg gcacgcggat
      601 actgggtgtg aaggaataca actgcgataa acctgagtgc ccccacgtca gatacgtcac
      661 taactcatgt ggcagccgtg cctgcccgtc ctgcggaaaa aaggccacag acctgtggat
      721 agcgacacag ctgaatcgtc ttcctgactg cgactgggtg cacctggtct tcaccctgcc
      781 ggacacgctg tggccggtgt tcgaaagcaa ccgctggctg ctgaatgacg tgtgccgtct
      841 ggcggtggag aatctgctgt atgcagcccg gaaacggggt ctggaacccg gtatcttctg
      901 cgccatccat acgtatggcc gtcgtctcaa ctggcatccg catgtgcatg tgtcagtgac
      961 ctgcggaggt ctgaataaac acggacactg gaaaaagctg agcttcctga aagacgcgat
     1021 gcgttcgcgg tggatgtgga atatgcggca gctgcttctg aaagcgtggt cagaaggtct
     1081 ggcgatgccg gaatcgttgt cacatatcac gacggaatca cagtggagaa gcctggtgct
     1141 gaaagccggc ggaaaatact ggcatgtgta catgtcgaag aagacagccg gagggcggaa
     1201 tacggcacgc tacctgggtc gttatctgaa gaagccacca atagcggctt cccggctggc
     1261 gcattacaac ggaggagcga gtctgagctt ccgttacctg gaccacaaaa cgggagaaac
     1321 ggcgacggaa acgctgacac agcgggagct ggtcgcgagg ctgaaacagc acatcccgga
     1381 gaagtttttt aagatggtga gatacttcgg gttccttgcc aaccgggtgt gtggagagaa
     1441 gctgccgcag gtgtaccgtg ctctggggat ggataagccg gaaccagtgg cgaaagtgtg
     1501 ctatgcacaa atggtgaaac agttcctgag tcgtgacccg ttcgaatgcg tgctgtgtgg
     1561 cggccggatg gtataccgcc gggccattgc ggggctgaat gtatcagggc tgaagaaaaa
     1621 cgcgcgggat atcagtctgc tgaggtatat gccggcctga cacaggctaa gtgtgcccgg
     1681 gaatcggtgc aggtataaaa aacagcctgt tacggtataa aaatcgacca tgcagagaga
     1741 gaaaccaccg gagtggaggg aaaagcagaa aggctcacgt ggcataaggc gtggacagcg
     1801 gtatcgtctg gtttttcaat ttcctatacg tgaacaacag cctggtgcat ggcatctttg
     1861 tctgtgattc gttcaccaaa actgcgacta tgtcaacgcc acgatgtttg accgttattt
     1921 gccattttca tgcccgctaa atactttcgt tactgatgct gtccgctttg caccatttca
     1981 tcatcactga ttggcgttgt gtttttggtc agcactccag ctttacgttt ctcttttaac
     2041 cggtaactct ctccttgttc tttttgacgg acgcatttta cgtttgggct ggatgtagta
     2101 acgcagcatg gaacgtccgc ccgtataacc cattgcctta atctccgcga agataacctc
     2161 gctattccag acattctctg ccaggcgcat gtcgatgtaa tccataaacg gtttcagctt
     2221 aaccattttg tggcgggtct ttctggctgg cggttcaggg tatttgaggt agcgtctgac
     2281 cgtccgttca gagcaaccaa tctgagccgc aatatcgaca atgtacgcgc cctgctggcg
     2341 catttgcttt atcatgtaaa agtcctctct gctcagcatg ttgatgtcct ttctggtgtg
     2401 agaaccttaa ggaaacaaca tgttgggtgg agcggacaat ccaaatggtg aattaccgtc
     2461 ttatatcact ggcgctgaca cctgtagttt gtatacacaa tccctggcat tgaaacacac
     2521 atatgaagtg tacttcggat atgaccgtac gctcaggaag tacggcagat tccgtcaaaa
     2581 attacctgta aagacagaca tcagaaaaaa cagtgctcgc cgcagaccgg tgaccgggcg
     2641 aagcccgcga accggcgagg aagcgcaatg gatctgtagt tacagtgagc aggtacgccc
     2701 tgaagccttc ataatcggta gtgtatgctg tttttctgga atgatttatc cacatatcca
     2761 gaggcccgat ccagtaaaca gatccatgaa tgatcaacaa aggatccatt aaagatcccc
     2821 atatcgctgt caaccttgtc actcatgggc cgggaccacg ttcacataag cggtggcatg
     2881 ttatggataa actgtaacag gttcatgata agctgtattc agtaatgcat atactgaagt
     2941 aagttaatga cataaactat ggtcagtacg ccagactcag ttgttaaata cagactgcag
     3001 gtttttcttc agtcagttga cggggttctg acacacgatt ttctgtttat tcttttactg
     3061 tccacaggca ggaggctttc tggaaaacga aaattcagac atcaaaaaac tgttcggcga
     3121 ggtggataag tcgtccggtg agctggtgac actgacacca aacaataaca acaccgtaca
     3181 acctgtggcg ctgatgcgtc tgggcgtctt tgtaccgacc cttaagtcac tgaagaacag
     3241 taaaaaaaat acactgtcac gcaccgatgc cacggaagaa ctgacacgtc tttccctggc
     3301 ccgtgcagaa ggattcgata aggttgagat caccggcccc cggctggata tggataacga
     3361 tttcaagacc tgggtgggga tcattcattc ctttgcccgc cataacgtga ttggtgacaa
     3421 agttgaactg ccttttgttg agtttgcaaa actgtgtggt ataccttcaa gccagtcatc
     3481 ccgcaggctg cgtgagcgca tcagcccttc cctgaagcgc attgccggta ccgtgatctc
     3541 gttttcccgt accgatgaga agcacacccg ggaatacatc acccatctgg tacagtcagc
     3601 ctactacgat actgagcggg atattgtgca gttacaggcc gatccccgcc tgtttgaact
     3661 gtaccagttt gacagaaaag tccttctcca gcttaaggcg attaatgccc tgaagcgacg
     3721 ggagtccgcc caggcactct acacctttat agagagcctg ccccgggatc cggcaccgat
     3781 atcgctggcg cggctgcgtg cacgcctcaa tctgaagtct cctgtatttt cccagaacca
     3841 gacggtcaga cgggcaatgg agcagctgcg cgagattgga tatcttgatt acacggagat
     3901 ccagcggggg cggacaaaac tcttctgcat tcactaccgg cgtccccggt taaaagcacc
     3961 gaatgatgag agtaaggaaa atccgttgcc accttcacct gcggaaaaag tcagtccgga
     4021 gatggcggag aagcttgccc tgcttgaaaa actgggcatc acgctggatg acctggaaaa
     4081 actcttcaaa tcccgctgaa cataaactgt agtcagtgaa gagtgctcct ttactgacta
     4141 cagcttatat tatcaggtgc agtgagtggt ctgctcactg cagtttatat tcagtttcat
     4201 gcagtgctgc ctgtggctga gctgccatct gcctgtccct tacgtgagtc accccgtaac
     4261 ctgatgctga ggcattgctc ccttcataaa acatggttta ctcactacag cttatctacc
     4321 tgctccagct tatgttatgt ctgttctgct gaccactgct tatataagga ggtgccgtta
     4381 cttcagggga ggcagtgtac gcaggatctc cgcagcatcc cgcccgtcac ctgtgaaagg
     4441 cactgccagc gtggctgcca tatccagtgc aaacactctg gtataaacct ccatagaacg
     4501 tggatccctg tgaccagcca gtgcctggat gactttccgg ggctggcggt gatagagcat
     4561 gtgcatgata tagctgtgcc ggaaggtgtg tggtgtgacc ggaatcgaaa agtgtactcc
     4621 gtcagcttcg gcccgtctga cagcctgctt cagccagttg cgcatggttt cgtcggtcac
     4681 ggcccataat ggttcacgac gacggggccg ggtggtgatc atccagcttt ccatctgcct
     4741 gacatagctt atatccgtca gcggaaccag gcggacttca tcttttggcg ggcgtccgcg
     4801 tcgcgcacgc actttttcgg acaggatccg cacaaacggt cttactccat ccaggtcaaa
     4861 tgattccggt gtcagcatcc gggcttcgcc aatacgcatt ccggtattcc agagggtggc
     4921 gaacagcata tggtgacgct gatccggcat atagaaaaga agggcactca cttccggggc
     4981 cagcaggtag gccggtgtgg cccctgtgga agtggccatt cttctcaggg agagcgctgt
     5041 tgcaaaatcc acccccggcg caatgggtaa cagggagacg cgttctggtg aattctgcag
     5101 gggaatgaca ttgttcatga gttatttttc acgtagcaca cgcactgttt atggatacag
     5161 gtattctgac ctgaacgccc ggatccttcc acgacaataa atcgcagcgc gcggatcctt
     5221 ttgtttgctt atcacgcatt attgcgggta aaaccaaata cgtaaaggcg cgttttgccg
     5281 cagggatcct gtcttaagcc agtcatggtg cgggctgagg actggtttgc cgtttttggg
     5341 agaattgggg taatgccagg aacaggcaga tacggagaaa aatcctgcag ataaccttct
     5401 acgacaactg aaggtgtctg acgccatatt cttagctact tctcagtttt aaggcggctt
     5461 agtcacaaca taaggttatg cataataagc atattt
//
LOCUS       DAPHDX010000212         5437 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPHDX010000212 DAPHDX010000000
VERSION     DAPHDX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.215"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..343)
                     /locus_tag="RH058_004270"
                     /pseudo
     CDS             complement(102..343)
                     /locus_tag="RH058_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            522..1172
                     /gene="inaA"
                     /locus_tag="RH058_004271"
     CDS             522..1172
                     /gene="inaA"
                     /locus_tag="RH058_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV3139759.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(1226..1480)
                     /gene="yfaE"
                     /locus_tag="RH058_004272"
     CDS             complement(1226..1480)
                     /gene="yfaE"
                     /locus_tag="RH058_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV3139760.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(1480..2610)
                     /gene="nrdB"
                     /locus_tag="RH058_004273"
     CDS             complement(1480..2610)
                     /gene="nrdB"
                     /locus_tag="RH058_004273"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV3139761.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(2699..4984)
                     /gene="nrdA"
                     /locus_tag="RH058_004274"
     CDS             complement(2699..4984)
                     /gene="nrdA"
                     /locus_tag="RH058_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV3139762.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1319 a         1303 c         1432 g         1383 t
ORIGIN      
        1 gtaaatgggc tgtttactga tttccctgat aaggcagtaa aattccttaa taaagagtaa
       61 tgctactgca ggatggcgtt catgcgccat cctgtctcat atcaaatttt tccattcacc
      121 acgagttgtt ccgtacacgt ttgtagtaaa aacagtggac tgccttctac cggcttttct
      181 ttagccaaca acgaaatctg tcgatagaag gttggttcgt gaggaacgga cacaattcac
      241 ctgcaatgct tttcagcgtt aactctggtt gtaaggcgat gcctaatccc tgtcgaataa
      301 aattcatcgc tgtatctgac tgattaaatt cacattttat ctgctgggcg cactattagt
      361 ggtagtggct ggtggaatct atgcgctgca aatgaagggc aaaagcggtg tcacacctgt
      421 agcggcaaaa gaaattcatt aatacgacac gtttcattaa gattttcctc aggtaaccac
      481 ctatagtcat tgtcgttaat aaaaatgaca ggcgataggg tatggcagtt tcagcaaagt
      541 acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc
      601 gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa
      661 agcgcatgac gcatcatctg tttcattctg tacgttatcc gttcggccga ccaacgattg
      721 tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg
      781 tttttggtga agcggtgaaa attgagggtg aatggcgagc gttgctggtg actgaagata
      841 tggcggggtt catcagcatt gctgactggt atgcccagca tgcagtatcg ccttattctg
      901 acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagca
      961 ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg
     1021 cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga
     1081 ttaatcatga cttccgccag ctggaaaaat atctggagcc aatccccaaa gcggactggg
     1141 agcaggtgaa agcgtattac tacgccatgt aatcgttgta ggccagataa gacgcataag
     1201 cgttgtatct ggcactacaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc
     1261 aacaacaggg caaaatttcc cccggctgaa taaaggctaa cggttcggca atccagtcaa
     1321 cttgacctgc aaccaggcgt gtgcgacagg agccgcagta accttcgcga cactgatact
     1381 caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca tcctggcaca
     1441 gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat cagagttgga agttactcaa
     1501 atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga cttccacttc
     1561 ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccatg ggatcgggtt
     1621 ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta
     1681 ttcaacgtac tggcagagaa tatctttatt cagaccaatc atcgaaccgt cacggaacag
     1741 ataatccgcc cagtctttct cctgttgagc tgcctgaaca aacaggtcat agcactcctg
     1801 cttacactct tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc gcagcagatt
     1861 cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat
     1921 tttggcgttg ccttccatca attcgcgttc tgcaaaggca aaggaacaag caaagctgac
     1981 gtagaagcgg atcgcttcca gcgcgttaac gctcatcagg caaagataca gttttttctt
     2041 cagctcgcgc aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag
     2101 aaggtgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga tcccttccgc
     2161 acgtttctgg atctgctcgt tggtgacgat atcgtcaaac acaacagacg gatcgttaac
     2221 gatattacga atgatatgag tataggaacg ggaatgaatc gtttctgaga acgcccaggt
     2281 ttcgacccag gtttccagtt ccggaataga aataagcggc aatagcgcca cgttcggact
     2341 acgaccctga atggagtcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt
     2401 ttcgtgctcc ggcagcgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg
     2461 acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg
     2521 atcgtagcga gccacgttga ccggctgacc aaaaaacatc ggttctttaa gctgatcatt
     2581 tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc tgtggtttta ttacccctca
     2641 ccctaaccct ctcccgcaag gggagagggg acggtttggt gcaaaggctg ggggattatc
     2701 agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggcacc agatcgtcct
     2761 gcgcgtcttc agcgccgtca cgggtgttct gataatacag cgttttgacc ccgaatttgt
     2821 aggcggtgag caggtctttc agcaactgct gcatcggcac ttttcctgac gggaaacgtg
     2881 acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc atgataccta
     2941 ccagttgcag gtagccatcg ttacccggca tttcccacag cagctcatag gcgtcgtgca
     3001 ggtgctcgta gtccggcacc acctggcgca aaataccgtc tttcgacgct ttgatgctga
     3061 cgtaaccgcg cggcggttca ataccgttag tggcgttaga gatctgcgaa gaggtctcgg
     3121 acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt gattcacgca
     3181 atgcttccca gtcgtaatgc agcggctcat tagcgatggc atccagatct ttcttatagg
     3241 tatcgatcgg caggatccct ttcgcgtaag tggtttcgtt aaaccacggg cacgcgcctt
     3301 gctctttagc cagctcatta gacgctttca gcaggtaata ctgaatcgct tcgaaggttt
     3361 tatgcgtcag gttgttggcg ctgccgtcgg agtagcgttt accgtgcttc gccagatagt
     3421 aagcgaagtt aatcacacca atacccagcg tacgacgccc catcgctcca cgtttggcgg
     3481 ccgggatcgg gtaatcctga taatccagca gcgcgtcaag tgcacgaacc gccagaattg
     3541 ccagctcttc cagttcatcc aggctattaa ttgcgcccag gttgaaagca gacagcgtac
     3601 acagcgcgat ttcaccgttc tcgtcgttga cgtcgttcag cggtttggtc ggcagggcta
     3661 tctccaggca caggttagac tgacgcactg gcgcgatggc cggatcaaac gggctatggg
     3721 tattgcagtg gtcaacgttc tgaatataga tacgaccggt agacgcacgt tcctgcatca
     3781 tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg tctttctcat
     3841 atttggtata caggcgttca aactcttcct gatcggcgaa gaatgcgtcg tacagccccg
     3901 gtacgtcgga cgggctgaac agggtgatat cttcaccttt cagcaggcgg gtatacatca
     3961 gtttgttgat ctgtaccccg tagtccatat ggcgcacgcg gttgccttcc acaccacggt
     4021 tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag aacagcgttg
     4081 ccgcaccgcc gcgcacgccg ccctgagagc aggatttcac cgctgtctgg aaatgtttgt
     4141 agaacggaat acagccggta tggaacgctt caccaccgcg aatcgggcta cccagcgcac
     4201 gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtatttc acaatcgcgc
     4261 tggaggtagc gttgatggaa tccagactgt caccgcactc gatcagtacg caggagctga
     4321 actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa attttaaatg
     4381 tggaaaccgc gtcgtaaaaa cgcttcacat attgcaggcg cgtttcacgc gggtagttcg
     4441 agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag atttcgccgg
     4501 tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca taagagaagg
     4561 tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct tccgtgtagt
     4621 cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccacg tggtcgtaca
     4681 gcgcaggcgg ctcaaactgg ccgtaggctt ttttacgcag gtggaagatc gccaggcgcg
     4741 cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggcc gccttgataa
     4801 tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg gagcgcagct
     4861 cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga acgcgatgga
     4921 ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc agattctgat
     4981 tcatgtatgt cgtacctgtt tttggaattc tttccgcaat agtttcatgc agactacctg
     5041 tagtacggga agctgcttag aacactatat atagggggta tgtttgggat tcactgcaag
     5101 atagtgtgaa aatggccctc ttgcaagtgc ataactttgt ggataactca ggaaggaaaa
     5161 agtggctttc gcgcacctta ggtcagacaa ggtgtccggg aaagtcaatg ggaagagaaa
     5221 aatttgttaa aaataactgt tcggcgattt cttgaacggc agaataggtt ggataaattg
     5281 atatagatgg tttattaatc tttttgctaa tgattgcaaa aattgatcta agcacgagtc
     5341 atcacataat ctgatgtatc agttgtggtc aatatataag cgaatgtaag cgattgttga
     5401 tgtcgaaatg gttgttttct attcggttat tgttaaa
//
LOCUS       DAPHDX010000213         5365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPHDX010000213 DAPHDX010000000
VERSION     DAPHDX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.216"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            96..686
                     /locus_tag="RH058_004275"
     CDS             96..686
                     /locus_tag="RH058_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3139763.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(784..1359)
                     /locus_tag="RH058_004276"
     CDS             complement(784..1359)
                     /locus_tag="RH058_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3139764.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(1359..2048)
                     /locus_tag="RH058_004277"
                     /pseudo
     CDS             complement(1359..2048)
                     /locus_tag="RH058_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(2160..2405)
                     /locus_tag="RH058_004278"
                     /pseudo
     CDS             complement(2160..2405)
                     /locus_tag="RH058_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            complement(2380..2925)
                     /locus_tag="RH058_004279"
     CDS             complement(2380..2925)
                     /locus_tag="RH058_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV3139765.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3314..3508
                     /locus_tag="RH058_004280"
     CDS             3314..3508
                     /locus_tag="RH058_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV3139766.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            3868..4161
                     /gene="iss"
                     /locus_tag="RH058_004281"
     CDS             3868..4161
                     /gene="iss"
                     /locus_tag="RH058_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDV3139767.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(4252..4434)
                     /gene="rzoD"
                     /locus_tag="RH058_004282"
     CDS             complement(4252..4434)
                     /gene="rzoD"
                     /locus_tag="RH058_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDV3139768.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(4584..4808)
                     /locus_tag="RH058_004283"
     CDS             complement(4584..4808)
                     /locus_tag="RH058_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3139769.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHNRDYLRSGYLHHRRPVMGC"
     gene            complement(4808..5023)
                     /gene="essD"
                     /locus_tag="RH058_004284"
     CDS             complement(4808..5023)
                     /gene="essD"
                     /locus_tag="RH058_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV3139770.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1466 a         1333 c         1185 g         1381 t
ORIGIN      
        1 ttcgaacgtg tacatacaaa tatgcacaaa aataatcaaa attattttct gagatgcatt
       61 atgatatgaa caccaatttc gtatagagtc tcactatgtc tcgaattttt gcttactgtc
      121 ggatatcaac gctggatcag accatcgaaa atcaacgccg ggaaatcgaa agtgcaggtt
      181 ttaaaatcaa acctcagcaa ataatcgaag aacacattag cggctcagca gcaaccagtg
      241 agcgtcctgg ttttaaccgg ttgcttgctc gcctgaaatg tggtgatcaa ttgattgtga
      301 caaaactgga tcgccttggt tgtaatgcaa tggatatcag gaaaacagtg gaacaactga
      361 ccgaaacagg tatcagagtg cattgcttag cattgggggg cattgacctg accagtccaa
      421 caggaaaaat gatgatgcac gtaatttcag cagtcgctga atttgaacga gaccttttac
      481 ttgaacgcac tcattccggg atagtaagag cccgcggcgc agggaaacgt tttggtcgac
      541 cacctgtgtt aaatgaagaa cagaaacagg tggtattcga acgaattaag tcaggtgtaa
      601 gtataagtgc cattgcccgg gaattcaaaa cctcgcggca aaccatttta agagccaaag
      661 caaaacttca gacacctgac atataaaaaa taatctcggt gtgagatgct ttacgtcttc
      721 caagccccct tccttgccgt aaatggaaag atacatctaa ttatagaatt tatatgtttt
      781 accctacggc agtgctggcc attcaatatc ctgtgcagtt gacgtatcaa cacggttcag
      841 caacacccga tactttttcc aggcttccag caatgaggtt tcttcctccg ttgcgatttc
      901 cagatctaca gcatcctgaa gtggcgcaat atgctcactg gctacctgca tcaggctgtt
      961 ttttgtttct tccgcctctc ggatccggaa cagtttttct gcttctgcat ctttcaccca
     1021 ggctgtgccg ttccacttct gaaactcccc ttccggcgat aaccaggtaa cattttccgg
     1081 taacggaccg agttcagaaa taaataacgc gtcccctgac gctacgtcat aaaccgtttt
     1141 accccgatgg tcttcaacga gatgccacga tgccttatca ctgttgaaaa cagccacaaa
     1201 gccagcagga atatctggtg gtgcaatatc ggtactgttt gctggcagac ctgtatgagg
     1261 cggaatatat gcgtcacctt caccaataaa ttcattagtt ccggccagca gattataaat
     1321 ttttatggtc cgtggttgtt cactcattct gaatgccatt atgcaagcct cacaatatag
     1381 ttaaatgcga tgtttttgac ggtgttttcc gcgttaccag cagcgttaac ggtgatggtg
     1441 tgtccatgtg aaccaatcgc aacggagtgc gtatgagcac caataccgac agtatgcgcg
     1501 tgtgcacctg cagatgctgc tgtgccggac agtgaatggc tatgattacc atctgtactg
     1561 gtattcgcta accaccccgt agacatacct actgagcctt gtacacccca ggtattttga
     1621 cctgagcttg tataaccata ttgataagta tctttaaaaa cactggggtt aaatcgacgg
     1681 ccatctctat ggctgtgatt accagctgca ttcgtgctgc cacttaaact atgggtatgc
     1741 gcaccagtgt tattcgtgga tttagtgccg taatcaaacg acgatgtggt tttcgtcccc
     1801 aaatccgtac tggatgcgct ggcgctgtgg gtatgcgatt taatgccgtc ctgttcctga
     1861 gacaatacgg cccgaccact ggcaggtttg cccttaatcg tccagccacg catatcaggg
     1921 atcacgcctg acggataagc ggctgcaagt ttcgggtatg cagatttgtc aaaagtctgc
     1981 ccctgcatca gggcatagcc agacggaacg gtatctgatg gccacgggat tggtgcgcca
     2041 ggcggataaa actgctctga tggcgtatag agtgaataaa ctgtaccgtc cgttaaccct
     2101 tccggcttat tagcagaata tgctggtgac gtatgaatcg tcacgttggc attactggta
     2161 taaccgacac gggcagactt caccacattc acctcgcgga tgtagtcgct gcccatcgca
     2221 ttcatgatgg cccgctgaaa gggcagtgtt tcccagcgcc cttcctggta tgcggattct
     2281 ttcgggagat agtaattagc atccgcccat tcaacggcgg tctgtggctc cggcctgaac
     2341 agtgagcgaa gcccggcgcg gacaaaatgc cgtagcctgt taacctgact gttcgatata
     2401 ttcactcagc aaccccggta tcagttcatc cagcgcggct gctttgttca tggctttgat
     2461 gatatcccgt ttcaggaaat caacatgtcg gttttccagt tccggaaaac gccgctgcac
     2521 cgacaggggg atcccgtcga gaatactggc aatttcacct gcgatccgcg acagcacgaa
     2581 agtacagaat gcggtttcca ccacttcagc ggagtctctg gcatttttca gctcctgtgc
     2641 gtcggcctgc gcacgcgtaa gtcgatggcg ttcgtactca atagtccctg gctggagatc
     2701 tgtctcgctg gcctgccgca gttcttcaac ttcccggcgc agcttttcgt tctcaatttc
     2761 agcatccctt tcggcatacc attttatgac ggcggcagaa tcataaagca cctcattacc
     2821 cttgccaccg cctcgcagaa cgggcattcc ctgctcctgc cagttctgaa tggtacggat
     2881 actcgcaccg aaaatatcag ccagctgctt tttgttgact tccattgttc attccacgga
     2941 caaaaacaga gaaaggaaac gacagaggcc aaaaagctcg ctttcagcac ctgtcgtttc
     3001 ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa
     3061 cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac
     3121 ctgtcggatc gccggaaagg acccgcaaaa tgataataat tatcatctac atgtcacaac
     3181 gtgcatctac gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat
     3241 tgatctgcat caacttaacg taaaaaaaac ttcagacaat acaaatcagc gacactgaat
     3301 acggggcaac ctcatgtcaa cgaagaacag aacccgcaga acaacaaccc gcaacatccg
     3361 ctttcctaac caaatgattg aacaaattaa catcgctctt gagcaaaaag ggtccgggaa
     3421 tttctcagcc tgggtcattg aagcctgccg ccggagacta acgtcagaaa agagagcata
     3481 tacatcaatc caaagtgatg atgaataaac atcccggttt cttccaccat cgcaccggaa
     3541 aagcgactat gagggtaacc ctgcgtctgt cagcacagta aaacccggtg tgcatcgttt
     3601 ttgattattc ccgcacactc acgcagaagg aattccccgt cgggctacgg tcatggttaa
     3661 tgcgggaata cggcgacgat acagcgcagc taaaagggta atggacagaa agagcggttt
     3721 atttcattcc acaggattct gagtgccccc ctcctccaat aggctgagca tccacctata
     3781 tagttttaat tttcatcaat ccatttaact atcgtttaat tgttgtcaca taggattctg
     3841 ccgtttttaa caatgcagga taataagatg aaaaaaatgt tgttttctgc cgctctggca
     3901 atgcttatta caggatgtgc tcaacagacg tttactgttg gaaacaaacc gacagcagta
     3961 acaccaaagg aaaccatcac ccatcacttc ttcgtttcgg gaattggaca ggagaaaact
     4021 gttgatgcag ccaaaatttg tggcggcgca gaaaatgttg ttaaaacaga aacccagcaa
     4081 acattcgtaa atggattgct cggttttatt actttaggca tttatactcc gctggaagcg
     4141 cgtgtgtatt gctcacaata attgcatgag ttgcccatcg atatgggcag ctctatctgc
     4201 actgctcatt aatatacttc tgggttcctt ccagttgttt ttgcatagtg atcagcctct
     4261 ctctgagggt gaaataatcc cgttcagcgg tgtctgccag tcggggggag gctgcattat
     4321 ccacgccgga ggcggtggtg gcttcacgca ctgactgaca gactgctttg atgtgcaacc
     4381 gacgacgacc agcggcaaca tcatcacgca gagcatcatt ttcagctttc gcatcagcta
     4441 actccttcgt gtattttgca tcgagcgcag caacatcacg ctgacgcatc tgcatgtcag
     4501 taattgccgc gttcgccagc ttcagttctc tggcattttt gtcgcgctgg gctttgtagg
     4561 taatggcgtt atcacggtaa tgattaacag cccatgacag gccgacgatg atgcagataa
     4621 ccagagcgga gataatcgcg gttatgcccg tgacatacag tccatacacc aacaatatct
     4681 ttatatggta tgtagctgac accttccaga ccatcgtcac cactcggacc agtgatgagc
     4741 acagacgcta tggcaacagc cccaccacca atagcagctg ctacagcctt gcgtaatgat
     4801 ggcgacatta ttcacctctc gcagccttgc gcttatcttc tttaatcttg aaataaaggt
     4861 ttgtcaggta cgtcagcagg ccaaatacca ggctacccag cactccaatt gccgcccact
     4921 gtgagggcgt gactttatct agcagctgta aaaaccagta cccggcacta cctgctgagg
     4981 tgccataggc gacacccgtt gttaacttat ccatggattt cataacccca cctcgcagac
     5041 aaagcgggtg taaattgagg gaatactacg aaacgtaaca gactcggagt cagtgaataa
     5101 ctcaggtatt gggttatcag ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg
     5161 gcgggtttaa gctgtgtgac gaagtaacca ctcttaacag cataaccaat tttttacgta
     5221 cgtaaaccac taaatgatat ttgcgagaat gctaccgagt attgaaaaca ccactacaaa
     5281 tacataagca aatctcaaca aataaccaac aaattatttc cagtgttatt tttagcttat
     5341 ttaaattgaa ccttcaaatt ataga
//
LOCUS       DAPHDX010000214         5295 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPHDX010000214 DAPHDX010000000
VERSION     DAPHDX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.217"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..1484
                     /gene="mltD"
                     /locus_tag="RH058_004285"
     CDS             126..1484
                     /gene="mltD"
                     /locus_tag="RH058_004285"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV3139771.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1532..2302)
                     /locus_tag="RH058_004286"
     CDS             complement(1532..2302)
                     /locus_tag="RH058_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3139772.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2380..3180)
                     /locus_tag="RH058_004287"
     CDS             complement(2380..3180)
                     /locus_tag="RH058_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV3139773.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3421..4335
                     /locus_tag="RH058_004288"
     CDS             3421..4335
                     /locus_tag="RH058_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3139774.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="RH058_004289"
     CDS             complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="RH058_004289"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV3139775.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1385 a         1278 c         1317 g         1315 t
ORIGIN      
        1 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
       61 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
      121 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
      181 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
      241 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
      301 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
      361 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
      421 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
      481 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
      541 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
      601 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
      661 gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
      721 aaggtcgggt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
      781 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
      841 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
      901 ctctggcgcg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca gatatggcgg
      961 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
     1021 caagtgggcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
     1081 tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
     1141 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagcatcgct tcacgtctcg
     1201 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag ctgaagccag
     1261 gccaaagttt gacgattggt gcaggcagta gcgcacagcg actggcaaac aacagcgata
     1321 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
     1381 tgaacatcaa agatgtgatg cgttggaaca gcgacactgc gaatctgcaa ccaggcgaca
     1441 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agttaataaa
     1501 aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgtgct tctaccatga
     1561 tgatatcact ggtgaaagag ccgtcattct gcaaggcaaa atacgttctc acctctgtcg
     1621 atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc ttctggcgta cgcattctcg
     1681 cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat
     1741 tggcttcatt gattaacctc aaccactcac cgctggcgta gtttcgtacg tgagaggtat
     1801 cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac tggatgaccc ggagacatta
     1861 cgtccatcac aatcatccta ccgccaggtt tcaatatccg gttcacttca cgcagtgctg
     1921 caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttat
     1981 cggcaaatgg cagactttcg gcatatccct ggcgggtggt gatatttttc agttgccggg
     2041 cttcggcagc ttgtgccacg acatccagca tttgagcaga taagtcatac gccaccaccg
     2101 tgctcacgtt ttgcgcagcg acaaagctgg catgccctgc tccgcagccc atatcaagta
     2161 cacttgcacc aggataatca gccagacgca ccgccaggcg ctgtaaatct cgcccggatg
     2221 catgtacggt actggttaaa tactcactgg cctgcgagct aaactgcttt tctacgtggt
     2281 catggtggga ttgtgttgtc attgcgctgt ccttttgttg tttgagtata aaaggacagc
     2341 gccaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg
     2401 actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccaata cagaagcttc
     2461 gctgacgttc agaccacggt agaaaacaaa atcgagcggg cggccaaacg cccgacggcg
     2521 ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc
     2581 gttcatcctt ctacggctcc aggcattgaa atctcctgcc ataatgaccg ggccgctgtg
     2641 gtgagctatc tgatcaccaa taggaagtaa ctgcttacta tagacatcca cgcccagact
     2701 gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta atggatagac
     2761 cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca
     2821 atacactgga tgtgccgccg aaagggtcat tacgccagaa ggatgttgtg gcagtacgaa
     2881 agcgggtacc tgatcggcgg caagatagtt agcggtcgca aactgtacta actctggcgt
     2941 tgtctgcgct tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga
     3001 caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc
     3061 ggtacttaac ggttccccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg
     3121 taagatcctt tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac
     3181 ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt tctactgtta
     3241 tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt
     3301 acaccggtca gaattttgct cattgttgag aacattaaat cggatgacat atcacgcatc
     3361 ctgatggttt aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa
     3421 atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtcgaaag cggcagcttt
     3481 agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc agtgaaaaag
     3541 ctggagatga aacttggcgt tagcctgctt aatcggacca cgcgacaact tagcctgacg
     3601 gaagaaggcg agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca
     3661 gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttgcgcat cgatgccgca
     3721 actccagtgg tgctgcactt tctgatgccg ttgattaagc ctttccgtga acgctatccg
     3781 gaagtcactt tgtcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtt
     3841 gatgtcgcga tacgcgccgg tacgttaacg gattccagct tacgtgccag gccgttattt
     3901 aatagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa
     3961 acgatcgacg atttaaagca acatatttgc ctgggattca ctgaacccgc ttccctcaat
     4021 acctggccga tagcccgtag cgatggacaa ttacatgagg tgaagtacgg tttgtcatcc
     4081 aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc
     4141 gactacatga tcgacaaaga aatcgctcgc ggagaattgg tggagttaat ggcagataaa
     4201 gtgttgccag tggaaatgcc attcagtgca gtctattaca gcgaccgtgc ggtaagtacg
     4261 cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct
     4321 gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg
     4381 caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta
     4441 agattacttt ccaggttttc acgtttagta gaagaaggaa ttactgagta accttccccc
     4501 atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc gatacgagca
     4561 ataacctcat ctttcagggc cttaccatac gccagcgtca tataggaagt aatatggatg
     4621 ccgtgctgtt tagcccagac aaccacttta cggttttgca gataaggaga gagttcaatc
     4681 tggttagtag cgatgttttc agcgccaaca gcagcaatcg ttttttccat caacgggatc
     4741 gtgaagttgg aaataccgat ctcacgtgtc agaccttgtt ttttggcttc cagcagcgcc
     4801 tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga
     4861 tcaacataat cggtacgcaa tttttgcagg ctctctttca gacttgggat caatttgtct
     4921 ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca
     4981 ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt
     5041 gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga
     5101 cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt
     5161 cgttaactga ggtgattatg ggcttcagac tgttgcggaa aaagagcgta aaacgcagag
     5221 gatttttgcg attctggcaa taatagatat ccagacgaaa aaaaacctcg agcgttaact
     5281 cgaggcttct taata
//
LOCUS       DAPHDX010000215         5292 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPHDX010000215 DAPHDX010000000
VERSION     DAPHDX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.218"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            59..3496
                     /locus_tag="RH058_004290"
     CDS             59..3496
                     /locus_tag="RH058_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV3139776.1"
                     /translation="MPLFRKNLITVSLLLSVFPGISWADRVDVPVYVEESLGPNWQSM
                     TIGTTSTSTSGMVPPVAHGTRSIAIGNNAVAGTEIEKPQVGEAIAIGSKANATKEQSV
                     AIGADTNATGWGSVVIGGDDIRPVHGKTYGRLTIPNAFLASTASGTGSVAIGSRTLSG
                     GELSLALGSIALAKGDASTAIGATAEAQDERSTAIGFNALSMARSALALGSESSATSE
                     SAMAFGSRAIAGKSSSIALGTDAVVSVGDNNVALGAGSQDKAATSVSSASVRSSDGRL
                     VTYQGFAASDPSSVISVGAQGKERQIVNVAAGAVTENSTDVINGSQLYSVAKSLQDDI
                     NAVTSAGFALKDQGNNEVRQVSGSAIQITGDDNITTTATDSDDNKSLKISLNDDLTVG
                     HDKAGSITVNNAAGNSSISLNGGDGTISLTSPAASGDTSTTATLGVTTGQGGLTDADG
                     TTKTRLIYTPDGSSEPEELASMNDGLSFVGDDGNVINKKLNETLSVTGGAGADAEMTD
                     NNIRVDNREGGLRVRMTRNLRDLASAVFGNGDNITTITDNGMSVTTGTGDNVRTVSLG
                     KEGLNNGGNIITHVASGLNGKQLSEADGDTLTNAANIGDLKSALQDTTSALVNSGYGV
                     QADDGNKVTRPLGEHVSIAGDGRNITTMVQDGKLLVQLSEHIDTGKNGSVTVGDTVIT
                     DEGLSITGGPSVTKSGINAGDQVISGVQDGVDAHDAVNVSQLNQQINNATAATTWSLK
                     TESSDASVAVSSQTVEVKHGLNTRVSAVQTDDKGNYSYEIDVTGIPVEYTDSKGNPLV
                     NIGGKFYTRTEDPDSGNITLSPAEPARVRISSDQPLVLTSVADGAVTPESVDAVNGGQ
                     LASAGNIVAGKNVVWNNGKATLSPDTFSELTTADGSNRTTTGTIQTVPDNVADAVNML
                     NREGTKYFKASSSGPAAKAEGRNSVAIGQSAISRAENSIAMGNGAEVSGDAAAGSVAI
                     GSGSRAGRAHTGNYSLNGQVVAGRTGSNTPVMSFGQAGQERQLQHVAPGVLSAESTDA
                     VNGSQLYAVHRQTEGNTQAINNLGKKFDHLNDRVDELNRDIRGVGASAAAMSGIPQAY
                     LPGKSLMGLGVGGYGGESAVAIGVSRISDNGKLIMKMNAGQNSRGNFSVGAGVGWQW"
     gene            3517..4296
                     /locus_tag="RH058_004291"
     CDS             3517..4296
                     /locus_tag="RH058_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3139777.1"
                     /translation="MSFYKNILSGLMAVILTGCGNLSNVSPEGTTDEPVWPSPDRTMF
                     SHSGTQKGSWPVPEKLRLIKAGMNKDQIYQLAGRPHFGEGIAGVREWDYLFNFTGPDG
                     DYVCQFKILFDRHMDARSFFWKPENCSQEQEVKRYERTLQGDILFDHDSSVLTEKGRE
                     QLMIVAEELRKEGGRRFIIEGHTDHTGSEPYNNALSEKRAGTVKGWFISQGFDAGNIE
                     IRGKGDKEPLVVCENTGDEVMKECLRPNRRVVITAEEEGDR"
     gene            4386..4565
                     /gene="tnpB"
                     /locus_tag="RH058_004292"
                     /pseudo
     CDS             4386..4565
                     /gene="tnpB"
                     /locus_tag="RH058_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            4570..4820
                     /locus_tag="RH058_004293"
                     /pseudo
     CDS             4570..4820
                     /locus_tag="RH058_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4959..5131)
                     /locus_tag="RH058_004294"
                     /pseudo
     CDS             complement(4959..5131)
                     /locus_tag="RH058_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1424 a         1189 c         1469 g         1210 t
ORIGIN      
        1 ctgtttattc aaaaatcaat atatagtctg taacaccatt gttatttacg ggtgatgtat
       61 gcctttattc agaaaaaacc tgattacagt ttctctcctg ctgtcagttt tccccgggat
      121 atcctgggca gatcgcgttg atgttcctgt atatgttgaa gaatctctgg ggccaaactg
      181 gcagagtatg accattggaa ccacatccac aagcacatcc ggaatggttc ctccggtagc
      241 ccacgggacc cgttcaattg ccattggaaa taacgctgtt gctggcactg aaatagaaaa
      301 gccccaggtg ggtgaagcga tcgccattgg ctccaaggca aatgctacaa aagagcagtc
      361 tgtggcaatt ggtgctgaca ccaacgccac tggctggggg agcgtcgtca tcgggggcga
      421 cgatatacgg cccgtacacg gaaaaactta tgggcgcctg accatcccga atgcctttct
      481 ggcttcaaca gcctcaggaa caggttctgt tgccattggc agccgcacgc tttccggtgg
      541 ggagttgtcg ctggctctgg gctccatcgc ccttgcgaaa ggtgatgcct ccacggctat
      601 cggtgccacc gctgaagcac aggatgaacg ttccaccgcc attgggttta acgccctgtc
      661 catggccaga agtgcactgg ctcttggcag tgaatcatct gcaacctccg agagtgccat
      721 ggctttcggc agcagggcca ttgccggaaa atcatcttcc attgcattag gcactgatgc
      781 agtggtttcg gttggcgaca ataacgttgc cctgggggcc gggtcacagg ataaagcggc
      841 cacaagtgtg agcagtgcca gtgttcgctc atcggatggg cgtctggtca cctatcaggg
      901 ctttgccgcc agtgatccat cctctgttat ttctgttggc gctcagggga aggaacgaca
      961 gattgtgaat gtcgcagccg gtgccgtaac agagaacagt accgatgtca ttaacggcag
     1021 tcagttgtat tctgtggcca aatcccttca ggatgacata aatgctgtca ctagtgccgg
     1081 atttgcactg aaagaccagg gaaataatga ggtcagacag gtgtcaggca gtgcaattca
     1141 gatcaccggt gatgacaata tcaccaccac cgcgacggac agtgatgata ataaatcact
     1201 taagatttcc ctcaatgatg accttacagt ggggcatgac aaagcgggga gtataaccgt
     1261 caataatgct gcaggtaaca gcagtatcag cctgaacgga ggggacggga caatttctct
     1321 gacctcaccc gcagcttccg gtgatacatc caccacagca acacttggtg tgaccacggg
     1381 gcaggggggg ctgacggacg cagacggcac cacgaaaacc cgtctgatct atacacctga
     1441 cgggagcagt gaaccggaag aactggcctc catgaatgac ggtctctctt ttgtcggtga
     1501 tgacggaaat gtgattaata aaaaactgaa tgaaacactg tcagtgacgg ggggagccgg
     1561 cgcagatgct gaaatgaccg acaacaacat tcgtgttgac aaccgggagg ggggactcag
     1621 ggtccgtatg acccgtaatc ttcgtgatct tgccagtgcg gtcttcggaa acggagataa
     1681 cattacaacg ataactgaca acggtatgtc ggtgacaacc ggtaccggag ataacgtgcg
     1741 taccgtttct cttggtaaag aggggcttaa taatggcggg aatattatca ctcatgtggc
     1801 cagcggactg aacgggaaac aactgtctga agctgacggc gacacactga caaatgcagc
     1861 caatattggt gatctgaaat cagccctgca ggacacaacg tcagcactgg tgaacagtgg
     1921 ttacggagtg caggctgatg acggaaacaa agtcacccgt ccactgggag aacatgtcag
     1981 tattgccggt gatggccgta acatcacgac catggttcag gacggaaaac tgctggttca
     2041 gctttcagaa catattgata ccggtaaaaa tggcagtgtg acagtgggcg atacggtgat
     2101 aacagatgaa ggactgagca ttacaggcgg accatctgtc acaaaaagcg ggattaatgc
     2161 aggcgatcag gttatttctg gtgtacagga tggcgttgat gctcatgatg ccgtcaatgt
     2221 atctcagtta aaccagcaaa ttaataatgc cactgctgca acaacctggt ctctgaaaac
     2281 ggaaagcagc gacgcctctg ttgcagtatc gtctcagact gtggaagtca aacatggcct
     2341 gaacacacgt gtatcggctg tacagactga tgacaaaggg aattacagct atgaaattga
     2401 tgtgaccggt attcccgtgg aatataccga cagcaaaggc aacccgctgg tgaatattgg
     2461 cggcaagttt tataccagga ctgaagatcc tgacagtggc aacatcaccc tttctccggc
     2521 agagccggcc agggttcgta tcagcagtga tcagcccttg gtcctgacaa gcgtggcgga
     2581 cggtgcagta actccggagt ctgtggatgc ggtaaacgga ggacaactgg cgtcagcagg
     2641 gaatattgtt gccggcaaaa atgtggtatg gaataacgga aaagcgaccc tttcaccgga
     2701 tacgttcagt gaactgacca ctgccgacgg aagcaaccgt accacgacag gtaccataca
     2761 gactgtaccg gataatgtgg cagatgctgt caatatgctg aaccgtgaag ggacaaaata
     2821 tttcaaggca tcctcatcag gaccggcggc aaaagcagaa ggcaggaaca gcgtggccat
     2881 aggacaaagt gccatttccc gtgctgaaaa cagcattgca atggggaatg gtgcagaagt
     2941 cagtggtgat gcggctgccg gctctgttgc cattggcagt ggctcacggg ccggacgggc
     3001 acacaccggt aattattcgc tgaatggtca ggttgtggcc gggcgtacag gaagtaatac
     3061 cccggtcatg tcattcgggc aggccggtca ggaacgccag cttcagcatg ttgcaccagg
     3121 ggtattatcc gcagaatcaa cagatgccgt gaacggaagc cagttatatg ccgttcaccg
     3181 ccagactgaa gggaacactc aggcaatcaa taatctcggg aagaaatttg atcatctgaa
     3241 tgacagagtg gatgagctga accgggatat tcgcggagtc ggcgcatcag cagctgcgat
     3301 gagcggtatt cctcaggctt atctgccggg gaaaagtctg atggggcttg gtgttggtgg
     3361 gtacggtggt gaaagtgccg tggcaatagg ggtatcccgt atatcagata acgggaaact
     3421 tattatgaaa atgaatgccg ggcaaaattc acgcggaaac ttttctgtgg gagcaggtgt
     3481 gggctggcag tggtaacccg gggtaaggag tataagatgt cattttataa aaatatatta
     3541 tcaggtctga tggcggttat tctgaccggg tgcggtaacc tcagtaatgt ttcaccggaa
     3601 ggaacaactg acgaacctgt gtggccttct cctgacagga caatgttcag tcacagcgga
     3661 acccagaaag gctcctggcc tgtaccggag aagctgcgtc tgataaaagc cggaatgaat
     3721 aaggatcaga tatatcagtt ggcaggccgt cctcattttg gtgaagggat agcgggagtg
     3781 agggaatggg attacctctt taatttcacc gggcctgacg gagattatgt ctgccagttc
     3841 aagatccttt tcgacaggca catggatgca agatcatttt tctggaaacc ggaaaactgc
     3901 agtcaggaac aggaagtaaa aagatatgag agaacattac agggggatat tctgttcgat
     3961 catgattcat cagtgctgac agaaaaagga cgtgaacaac tgatgatcgt tgcagaagaa
     4021 ctcaggaagg aagggggcag gcgtttcatt atagaaggtc atacagatca tacggggtca
     4081 gaaccatata ataacgcact gtcagagaaa cgggccggca cagtcaaagg gtggtttata
     4141 agccagggtt ttgatgcagg aaatatagag atccggggta aaggtgacaa ggaacctctg
     4201 gttgtctgtg aaaatacggg ggatgaggtt atgaaggagt gtcttcgtcc aaaccgaaga
     4261 gtggtgatta ctgctgagga ggaaggagac aggtaaatag atctgatata cctgagcttg
     4321 ttatttatat gcttttattt ttcggggcgc gttagcgccc tttttattct tacccttaac
     4381 catctatggg ggatggtggt gattgccaat gcaagaatat gcgccggggc atcgacacac
     4441 tgatacagta cgtccagaac gaacttaacg ctgcatggca tgatggtgct gcttttgtct
     4501 tcaccaataa agcccgctcc cgcataaaag ttctccgttg ggataaatat gatgtatggc
     4561 tgtgtaaccg tgaagaaaat cgccgggttg taccgtcttg ataagaagat cagtagccgc
     4621 ctcgtggaaa aatccgccag tggcaacagc gttatgcccg tccgctactg gaagatctgt
     4681 ggtcgtggtt cgaggagcag taaccgaaat gttcccctgg cagggcatta cacaaagcca
     4741 ttgtctatgc gctgtctcat cgcgtggaac tgagccgctt cctggaagat ggcgcggtgc
     4801 cgctagataa taatgtgtgt taatgggcca tcaaaaacgt ggtcctgggc agaattacca
     4861 tactcagtac ttcaaggcct gtcggcgttt atcccgcaca ggacttcaac acccgatcag
     4921 ggtcacctcc gtcgcctgat caggttacaa tcgtcgtgtc aagatgagat gctaggaagc
     4981 ataccatcaa cacggttgga aggccattcc ggcaatacgc tcaggatatg gcgcagatac
     5041 gtttccggat cgataccgtt cagccggcag gtgccgatca gtccatgcac ggtacaccac
     5101 gctttccact gtaatagttg ccgaagaaca cagttatcaa atacatctgg attacttcaa
     5161 tacacaaaaa taatgaattt tatgtttgtt cgcattactg tgaaagaccg agagttattg
     5221 ggtagtctta tttcatggaa aatattaata acgtcctgtg gcgtatgaat aatacaatgt
     5281 tgattgcatg ta
//
LOCUS       DAPHDX010000216         5198 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPHDX010000216 DAPHDX010000000
VERSION     DAPHDX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.219"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="RH058_004295"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="RH058_004295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV3139778.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="RH058_004296"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="RH058_004296"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV3139779.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="RH058_004297"
     tRNA            2652..2727
                     /locus_tag="RH058_004297"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            2892..4076
                     /locus_tag="RH058_004298"
     CDS             2892..4076
                     /locus_tag="RH058_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3139780.1"
                     /translation="MPLNDMQIRRAKPEAKAYTLGDGQGLSLLVEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLALQTESENAFEK
                     IAREWHQQKSTKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEFNPAADLSSALNVHQSNHFPFLKANEIPDFLRA
                     LNGYTGSRLVLIATKLLMITGVRTIELRAALWSEFDLDNAIWEIPAERMKMRRSHLVP
                     LSTQALDLLNELKMMTGKYSYVFPGRNDPNKPMSEASINQVIKRIGYGGKLTGHGFRH
                     SLSTILHEKGYDSAWIEIQLAHIDKNNIRGTYNHAQYIDKRRDMMQWYSDYIFIKENV
                     NE"
     gene            4069..>5198
                     /locus_tag="RH058_004299"
     CDS             4069..>5198
                     /locus_tag="RH058_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDV3139781.1"
                     /translation="MSNEFDSSKLENCFELALENIIKHGDTDIFPYPFESRLFEDDKE
                     KVKTALMQTFNDFENKRIEIPPNIINSFSSIGYYGYRWATQIDPFWNAFFLGLVLKIA
                     DDIERNRSTKTQVYSYRFKPNLADGSLFDKEISWRKYQEDSISECSNDEIKYVLTCDI
                     ADFYPRIYHHRLENALDRVDPNKDYSGKIKKLLQTFSETKSYGVPVGCPASRILAELA
                     LDSIDKLLSMNRINYKRYVDDFVIFCNSREDAHKILTLLSKKLMENEGLTLQKQKTNI
                     VTKEEFLSVTKAKLHGNDEDEESPMKAKFMSLPIRFDPYSANAIEEYEEIKESLKDFD
                     LLAMLSSELQKSKINQSFSKHLIKAFSATSDEIISSAFKVMF"
BASE COUNT         1450 a         1111 c         1291 g         1346 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgcttgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg gtacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcagat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag caatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttcttgaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggcgtca gtcgttacaa cagcgatgca ttacgccgta ttgccgggca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagcagg ctgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgtgccaa tggccttggc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgatgat gtgcgccagg tgtgtaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cagcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg
     1741 ctgcaacggt ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc
     2161 agttgcaggc aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccatct ccacttcttc aaacatccgt atttatccat
     2761 aaccgcactg ttttataaaa gattttttgt ttttactgtt cgtattagtc cataactttc
     2821 cagtagaatc cagtactaaa tgtgtatagg attatgtata tgttcctgtt cgattttgga
     2881 attctataca catgccccta aatgatatgc agattcgccg tgctaaacct gaagctaaag
     2941 cctatacact tggggatggg caagggttgt ctttacttgt agagccaaat ggaagtaaaa
     3001 gctggcgatt tcgttatcgc tatgccggta aacccaaaat gatctcgctt ggtgtttacc
     3061 caacgatcac tcttgctgat gctcgttccc gtcgtgatga agctcgaaaa cttgtggcag
     3121 aaggaaagaa ccctagtgag gttcgaaaag agcaaaagct ggctctgcaa acagagtcag
     3181 agaacgcctt cgaaaagata gccagagagt ggcatcaaca gaagtctacc aaatggtcgg
     3241 cgggatatgc atcagacatc atggaagcgt ttaagaacga catttttcct tatgtgggaa
     3301 caaggccagt gggagagatt aaaccgctag aactgcttaa tgtgctgcgt aaaatcgaaa
     3361 agcgcggtgc attagaaaaa atgcgcaaag ttcggcagcg atgctcagaa gttttccgct
     3421 atgccattgc tactggaagg gctgagttta accctgctgc ggatctttca agcgccctca
     3481 atgtacacca atcaaatcat ttcccgttct taaaggctaa tgagatacct gattttcttc
     3541 gcgccttaaa cggatatacc ggaagtcggc ttgtcctgat tgccacgaaa ttgctcatga
     3601 ttacaggtgt tagaaccatc gaattacgtg cggcattatg gtcagaattt gatttagata
     3661 acgctatttg ggaaattcct gctgaaagga tgaaaatgcg cagatcacac cttgtgcctt
     3721 tgtcgactca agcgttagat ttgctaaatg aactcaagat gatgacaggg aagtatagtt
     3781 atgtttttcc ggggcggaac gatccgaaca agcctatgag tgaggcgagt attaaccaag
     3841 ttatcaagcg tattggttat ggtggaaaac ttactggtca tggatttcga cattccttat
     3901 ctactatcct ccacgaaaaa ggatatgatt cggcttggat agaaatacag cttgctcata
     3961 tagataagaa taatattaga ggtacgtata atcatgctca atatattgat aaacgccgtg
     4021 atatgatgca gtggtattca gattatattt ttattaagga gaatgtgaat gagtaacgag
     4081 tttgatagta gtaaactaga aaattgcttt gagcttgcat tggaaaatat tataaagcac
     4141 ggcgatacag atattttccc ttacccattt gaaagtcggt tatttgaaga tgataaggag
     4201 aaagtaaaaa ctgcattaat gcaaacattt aatgactttg aaaataaaag gatcgagatt
     4261 ccaccaaaca taattaatag cttttcaagt attggttatt atggttacag gtgggcgacc
     4321 caaattgatc cattctggaa tgcttttttt cttgggttag ttttaaaaat cgctgatgat
     4381 attgaaagga atagatctac taaaacgcag gtttattcat atcgctttaa accaaacctt
     4441 gctgatggtt ctctttttga taaagagatc tcttggagaa aatatcaaga agacagtatc
     4501 tctgaatgtt ctaacgatga aataaagtat gtacttacat gcgatatagc agatttctat
     4561 ccgcgtattt atcaccaccg tttagaaaat gcgttagata gagtcgaccc caataaagat
     4621 tactctggga aaatcaagaa attactacag acatttagtg aaacaaaatc atatggagta
     4681 ccagttggat gtcctgcctc tagaatatta gcagaactag ctctagattc tattgataaa
     4741 ttattgtcta tgaatagaat caactataag cgttatgtcg acgactttgt tattttttgt
     4801 aactctagag aggatgctca taagatttta actttgctta gtaaaaaact gatggaaaat
     4861 gaagggctaa ctttacagaa acaaaaaacc aatattgtta ctaaagaaga gttcctttca
     4921 gtaactaaag ctaagttgca tggtaatgat gaagatgaag aatctcctat gaaggctaaa
     4981 tttatgagtc ttcctataag attcgatcct tactcagcaa atgcgataga ggaatatgaa
     5041 gagataaagg aatctttaaa agattttgac ttgttagcta tgctgagtag tgagttacaa
     5101 aaatcaaaaa ttaaccaatc ttttagcaag catttgataa aggcattctc agcaacatca
     5161 gatgaaataa taagtagtgc tttcaaagta atgtttaa
//
LOCUS       DAPHDX010000217         5124 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPHDX010000217 DAPHDX010000000
VERSION     DAPHDX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.220"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..1360
                     /gene="garP"
                     /locus_tag="RH058_004300"
     CDS             26..1360
                     /gene="garP"
                     /locus_tag="RH058_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV3139782.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1376..2146
                     /gene="garL"
                     /locus_tag="RH058_004301"
     CDS             1376..2146
                     /gene="garL"
                     /locus_tag="RH058_004301"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV3139783.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2176..3066
                     /gene="garR"
                     /locus_tag="RH058_004302"
     CDS             2176..3066
                     /gene="garR"
                     /locus_tag="RH058_004302"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV3139784.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3163..4308
                     /gene="garK"
                     /locus_tag="RH058_004303"
     CDS             3163..4308
                     /gene="garK"
                     /locus_tag="RH058_004303"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV3139785.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4341..4717
                     /gene="rnpB"
                     /locus_tag="RH058_004304"
     ncRNA           4341..4717
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RH058_004304"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1198 a         1222 c         1470 g         1234 t
ORIGIN      
        1 agcataagta cccgaggtaa ataaaatgat tctggacacc gttgacgaaa aaaagaaagg
       61 cgtgcatacc cgctatttaa tattactgat tatttttatt gttaccgccg ttaactacgc
      121 cgatcgtgca acgctgtcta ttgctggtac cgaagtggca aaagagttgc agttaagtgc
      181 ggtttcgatg ggttacatct tttctgcttt tggctgggcc tacttgctga tgcaaatccc
      241 cggcggctgg ctgcttgata agtttggctc gaaaaaagtt tatacctata gcctcttttt
      301 ctggtcgcta ttcactttcc tgcaaggctt tgttgatatg ttcccgctgg cctgggcagg
      361 gatctccatg ttctttatgc gctttatgct cggcttctcg gaagcgccat cattcccggc
      421 gaacgcccgt attgtcgccg cctggttccc gacgaaagaa cgtggtactg cctccgccat
      481 ctttaactcg gcgcaatatt tctcgctggc gctcttttcg cctctgcttg gctggctgac
      541 tttcgcctgg ggctgggagc acgtctttac cgttatgggg gtgattggtt ttgtgctgac
      601 ggcgctgtgg atcaaattga ttcataaccc gacagatcac ccacgtatgt ctgcggaaga
      661 gctgaagttt atctctgaaa atggcgcggt ggtcgatatg gaccacaaaa agccgggcag
      721 cgcggcagca agcggtccca aactgcatta catcaagcaa ttgctctcta accgcatgat
      781 gttgggcgta tttttcggac aatattttat caacaccatc acctggttct tcctcacctg
      841 gttcccgatt tatctggtgc aggaaaaagg catgtcgatt ctgaaagtgg gtctggtcgc
      901 ctcgattccg gcactgtgtg gttttgcggg cggcgtgctg ggaggtgtct tctcggatta
      961 tctgatcaaa cgcggtttat ccctgaccct ggcacgtaag ctaccgattg tgctgggaat
     1021 gttgctggct tccaccatca tcttatgtaa ctacaccaac aacaccacgc tggtggtcat
     1081 gctaatggcg ctggcttttt ttggcaaagg atttggtgct ctgggctggc cggtgatttc
     1141 tgacaccgcg ccgaaagaga ttgttggcct ctgtggcggc gtctttaacg tctttggcaa
     1201 tgttgcctcc attgtcactc cactggtgat tggctacctg gtaagtgaac tgcactcctt
     1261 caatgcagca ctggttttcg tgggatgttc agcgctgatg gcgatggtct gctacctctt
     1321 cgtagttggc gacattaaac gtatggaatt gcagaaataa gcaaaggtat aagcgatgaa
     1381 taacgatgtt ttcccgaata aattcaaagc cgcactggct gcgaaacagg tacaaattgg
     1441 ttgctggtca gcactctcta acccgattag cactgaagtt cttggtttgg ctgggtttga
     1501 ctggctggtg ctggatggcg aacatgcgcc aaacgatatc tccacgttta ttccgcagtt
     1561 aatggccctg aaaggcagcg ccagcgcgcc agtagtgcga gtgccgacca acgagccggt
     1621 aattattaag cgtcttctgg atatcggttt ctataacttc ctgattcctt ttgtagaaac
     1681 aaaagaggaa gcagagcagg cggtggcatc aacccgttac ccaccggaag gcattcgcgg
     1741 cgtctccgtt tctcaccgcg ccaatatgtt tggcaccgtg gcggattatt tcgctcagtc
     1801 gaacaagaac atcaccattc tggtccagat agaaagtcag cagggcgtag ataacgtcga
     1861 tgccattgcc gctaccgaag gtgtagacgg tatcttcgtc ggccccagcg atctggctgc
     1921 ggcattaggc catctcggca atgcctcgca cccggatgta caaaaagcaa ttcagcacat
     1981 ttttaaccgt gccagtgcgc acggcaaacc cagcggtatt ctcgcgccgg tcgaagccga
     2041 tgcgcgtcgt tatctggaat ggggcgcgac gtttgtggct gtcggcagcg atcttggcgt
     2101 cttccgctct gccactcaga aactggctga tacctttaaa aaataaccac caccgcaaca
     2161 agagagatga ttgatatgac tatgaaagtt ggttttattg gcctggggat tatgggtaaa
     2221 ccaatgagta aaaaccttct gaaagcaggt tactcgctgg tggttgctga ccgtaaccca
     2281 gaagctattg ctgacgtgat tgctgcaggt gcagaaacag cgtctacggc taaagcgatc
     2341 gctgaacagt gcgacgtcat cataaccatg ctgccaaact cccctcatgt gaaagaggtg
     2401 gcgctgggtg agaatggcat cattgaaggc gcgaagccag gtacggtatt gatcgatatg
     2461 agttctatcg caccgctggc aagccgtgaa atcagcgaag cgctgaaagc gaaaggcatt
     2521 gatatgctgg atgctccggt gagcggcggt gaaccgaaag ccatcgacgg tacgctgtca
     2581 gtgatggtgg gcggcgacaa ggctattttc gacaaatact atgatttgat gaaagcgatg
     2641 gcgggttccg tggtgcatac cggggaaatc ggtgcaggta acgtcaccaa actggcaaat
     2701 caggtcattg tggcgctgaa tattgccgcg atgtcagaag cgctaacgct ggcaaccaaa
     2761 gcgggcgtta acccggacct ggtgtatcag gcaattcgcg gtggactggc gggcagtacc
     2821 gtgctggatg ccaaagcgcc gatggtgatg gaccgcaact tcaagccggg cttccgtatt
     2881 gatctgcata ttaaggatct ggcaaatgcg ctggatactt cccacggcgt cggcgcgcaa
     2941 ctgccgctca cagctgcggt tatggagatg atgcaggcac tgcgagcaga tggtttagga
     3001 acggcggatc atagcgccct ggcgtgctac tacgaaaaac tggcgaaagt cgaagttact
     3061 cgttaatgac gtcgtgcccg gatggcgtat tgcaatccgg gcctggaatc caggccgaat
     3121 aagagaaacg ccctccggcg tcatgtggta acaggcatag gtatgaaaat cgtaatcgcc
     3181 ccagactctt ataaagaaag tttatctgcc agcgaggttg cgcaggcgat agaaaaagga
     3241 tttcgggaaa tttttcctga tgcacagtac gtttctattc cggttgccga cggtggcgaa
     3301 ggaacggtgg aagcgatgat tgcagccacc cagggttctg aacgtcacgc ctgggttaca
     3361 gggccgctgg gcgagaaagt gaatgccagt tgggggatct ccggcgatgg caaaaccgcg
     3421 tttattgaaa tggcggcggc cagtgggctg gagctggtac ctgcggaaaa acgcgatcca
     3481 ctcgtgacca cttcacgcgg tacaggcgag ttaatcctgc aggcgctgga gagcggcgcg
     3541 acaaacatta ttatcggcat tggcggtagc gctacaaatg atggcggcgc aggcatggta
     3601 caggcgctgg gggcgaaatt atgcgacgcc aacggcaatg aaattggttt tggcggcggt
     3661 agtcttaata ctctgaatga tattgatatt tccggcctcg atccgcgctt aaaagattgc
     3721 gtcattcgcg tcgcttgtga tgtcaccaat ccgctggtgg gcgataacgg cgcatcgcgc
     3781 atctttggcc cacaaaaggg agccagtgaa gcgatgattg ttgagctgga caataacctc
     3841 tctcactatg ccgatgtcat taaaaaagcg ctgcatgttg atgtgaaaga tgtccccggc
     3901 gcaggagctg cgggcggtat gggtgcggcg ctgatggcgt ttcttggtgc ggaactgaaa
     3961 agtggtattg aaatcgtcac tacggcgctg aatctggagg aacatattca cgattgtacg
     4021 ctggtgatca ccggtgaagg gcgtattgac agccagagta ttcacggcaa ggtaccgatt
     4081 ggcgttgcaa acgtggcgaa gaagtaccat aaaccggtga ttggcattgc gggtagcctg
     4141 accgatgatg ttggcgttgt acatcagcat ggcatcgatg cggtcttcag cgtattgacc
     4201 agcataggta cgttggacga agcattccgc ggggcttatg acaatatctg ccgtgcttca
     4261 cgtaatatcg ccgcgacact ggcgattgga atgcgcaacg cggggtgaca agggcgcgca
     4321 aaccctctat actgcgcgcc gaagctgacc agacagtcgc cgcttcgtcg tcgtcctctt
     4381 cgggggagac gggcggaggg gaggaaagtc cgggctccat agggcagggt gccaggtaac
     4441 gcctgggggg gaaacccacg accagtgcaa cagagagcaa accgccgatg gcccgcgcaa
     4501 gcgggatcag gtaagggtga aagggtgcgg taagagcgca ccgcgcggct ggtaacagtc
     4561 cgtggcacgg taaactccac ccggagcaag gccaaatagg ggttcataag gtacggcccg
     4621 tactgaaccc gggtaggctg cttgagccag tgagcgattg ctggcctaga tgaatgactg
     4681 tccacgacag aacccggctt atcggtcagt ttcacctgat ttaacgaaaa cccgccagcg
     4741 taaaccggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacgcta
     4801 tacccaaaag aaagcggctt atcggtcagt ttcacctgat gtacgtaata aaccgttccg
     4861 gcgggtttca gattgttgag tgcgctttat tcatgccgga tgcggcgtga acgccttatc
     4921 ctgcctacaa aagcacgcaa attcaatata ttgcagagat ggcgtaggcc tgataagcgt
     4981 agcgcatcag gcatttttgc ggttgccatc agtttcaaac ccgcgtcagt gagtttttgc
     5041 ttttggtgca cagggtaaga tgaataaata tctacggtat taataataga ctttgtaaga
     5101 agttattaag tctatattgc attt
//
LOCUS       DAPHDX010000218         5036 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPHDX010000218 DAPHDX010000000
VERSION     DAPHDX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.221"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(12..470)
                     /locus_tag="RH058_004305"
     CDS             complement(12..470)
                     /locus_tag="RH058_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV3139786.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            590..1399
                     /gene="proC"
                     /locus_tag="RH058_004306"
     CDS             590..1399
                     /gene="proC"
                     /locus_tag="RH058_004306"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV3139787.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1416..2531)
                     /gene="adrA"
                     /locus_tag="RH058_004307"
                     /gene_synonym="dgcC"
     CDS             complement(1416..2531)
                     /gene="adrA"
                     /locus_tag="RH058_004307"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV3139788.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2633..2953)
                     /gene="psiF"
                     /locus_tag="RH058_004308"
     CDS             complement(2633..2953)
                     /gene="psiF"
                     /locus_tag="RH058_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV3139789.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3072..4487)
                     /gene="phoA"
                     /locus_tag="RH058_004309"
     CDS             complement(3072..4487)
                     /gene="phoA"
                     /locus_tag="RH058_004309"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV3139790.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4588..4848)
                     /gene="iraP"
                     /locus_tag="RH058_004310"
     CDS             complement(4588..4848)
                     /gene="iraP"
                     /locus_tag="RH058_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV3139791.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1183 a         1359 c         1230 g         1264 t
ORIGIN      
        1 ataaattaca tttagccacg actacgttgc acttccagcc accacttctc cagctccgcg
       61 gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc cgccggtctg gatcccactg
      121 gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg
      181 taacgttcgc cgcgcggatt aagcgccgct gcgcctttct cgatggcttc agcagccaaa
      241 ggtatatctg cggtgatcac caaatcgccc gcttcacact gccggacaat ttcgttatcg
      301 gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt
      361 aaactctggt ttgctaccag taccagcggc atctgcatac gttccgccgc gcgatacaaa
      421 atctctttaa ttacattggg acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc
      481 tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc
      541 ttctgccagc gattatcaaa acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa
      601 aatcggtttt attggctgcg gcaatatggg aaaagccatt ctcggcggtc tgattgccag
      661 cggtcaggtg cttccagggc aaatctgggt atacaccccc tccccggata aagtcgccgc
      721 cctgcatgac cagttcggca tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc
      781 cgacatcatt tttgctgccg ttaaacctgg catcatgatt aaagtgctta gcgaaatcac
      841 ctccagcctg aataaagact ctctggtcgt ttctattgct gcaggtgtca cgctcgacca
      901 gcttgcccgc gcgctgggcc atgaccggaa aattatccgc gccatgccga acactcccgc
      961 gctggttaat gccgggatga cctccgtaac gccaaacgcg ctggtaaccc cagaagatac
     1021 cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg gaagtaattg ctgagccgat
     1081 gatccacccg gtggtcggtg tgagcggttc ttcgccagcc tacgtattta tgtttatcga
     1141 agcgatggcc gacgccgccg tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc
     1201 cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa acgggagaac atccgggggc
     1261 actgaaagat atggtctgct caccgggagg caccaccatt gaagcggtac gcgtactgga
     1321 agagaaaggc ttccgtgccg cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga
     1381 aaaactcagc aaatcctgat gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta
     1441 cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg atttcaacca ctcacgatag
     1501 tgactcattt gtgggttcag cggcgcaacc cccacactaa tccgtaaagt tacctgtggc
     1561 gtattcggca agcgtaacgt gtttagccct tcacgcaccc gtaacatggc ggtaatggcg
     1621 ctctcagctg gcgtaccgga catgattact gcaaactcat cgccgccaaa ccgaccaatc
     1681 acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg
     1741 cccacatcat gaccccaggt atcgttgatg ctcttgaaat ggtcgatatc gataatcagt
     1801 aacgttgcat cgcgattatg ccgccgacag ttatcaaatt cattgcgtaa catagtttcc
     1861 caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg tactcatgac ctgcaacctg
     1921 cgtttatgtt ccgccagttt ggttgccgtc tggtagctga cccagccaaa cagcagagga
     1981 taaacgacaa taatgggaag ggagagccac cattccagcg gcgcactatt gaacgacacg
     2041 gtaatgcccg taagctcgag ggtgacaagg caggaaacca ccatcaacac cagacccgcg
     2101 acaaacagac gggggccgcc tgcccccatc aaattcagac acataatcat caacatagcg
     2161 gtggaaggca gcacgtttac gcccattacg cctacccaca ttcccgctaa tactgcatcg
     2221 gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg ccctgctcgc tatctgccag
     2281 gctaaatgcg gccagacgaa cgcccagccg accaacacca gccaccacca gcccggcggc
     2341 gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc cagccaggcc aaccgcacgg
     2401 ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt
     2461 aaaggaggtt cctccccatg cgccgccgct tttttgaaaa agttttcatc attcattatt
     2521 tttgggaaca ttcagaaaca attttcccaa attatagaga cggggctaaa ggcagaagta
     2581 tgaaatttcg ggtgagccac tttcgtagct cacccgttag cactatcagg tatcaggcgg
     2641 ctttcttgag gcaggcactc ataaacttat tacgatcatc acctttcaga gattgttgcg
     2701 ttgcttgatt attacattcg cgcatctttt gctgctgtgg cgtcaaactt ttttcaccag
     2761 gcgcagactt gctgttcttc aggcaatcac tcatgtaggt cttacgagca tcccctttca
     2821 acgcctgcgc cgtcgcctgc tgattacagg atgtcatacg ctgttgttgt ggggttaagg
     2881 ttctctcggc agcgccgacg gtggttaaaa aaaccagacc aaaaagcaag gtaaccagta
     2941 atgttatttt catagcacca tccctcttca tgttttaacc atgagcgtat gcgcccgtga
     3001 tctgccatta agtctggttg ctaacagcaa aaaaaccacc cggcagcgaa aattcactgc
     3061 cgggcgcggt tttatttcag ccccagggcg gctttcatgg tgtagaagag atcggtctgg
     3121 tcggtcagtc caacaacatt ggcggcatgc gggccatacg ccgcaatacg caactgactg
     3181 ccggtatgtt cttgtgaatc ctcttcggag ttcccgtagc tcatcaccat cactgcgcca
     3241 tctttggtat ttagcgcctg ggtgaggccc ggagctttgg tatccggcgc gacaatctgg
     3301 ctggcgtggg cgtgatcagc ggtgactatg accagcgtat tgccatcctt tttagcgaat
     3361 tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct cgccaatttg cccacaagga
     3421 ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa cttgcaggaa aaatcctttc
     3481 tcatttttac tcaacaattc aatggctttg tcggtcatct gcgccagggt tggtacactg
     3541 tcattacgtt gcggattagg cgtacaggtg actgcgggct tatcgatatt gccgtggtac
     3601 gttgctttcg gtcctagcca gcgcactggc atattgccgt cagcaaacag tccaagcaga
     3661 ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg cagcatcgct gaccaactga
     3721 taaccacgcg cctgtgcctg ttcacgcagc gtttttccct gccattcacc ggcggttgcc
     3781 gtttcagcaa aggtttttgc gccaccgcca agcgtaacgt cggcacgagc gttaagcagc
     3841 tgttcggtaa tcgatccttt tccgcctttt tccagagcgt tacccggaca tttttcactg
     3901 gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg ccaccagcgc agcgggcgtg
     3961 gcatcctgca actctgcggt agaaacgtta ccggtcgcca gacctgcggc ttttgccatt
     4021 tccagaatcg ttgggtgatc tttttcgtga atatcgacgc ccagcgcgcc gttataggtt
     4081 ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt cggtgacgta gtccggtttg
     4141 ccggtttttt tattcagcgc atagtgagtg tattgcccgg taagcggtaa ggcatcgata
     4201 cctttaaaaa agccgcccgc accttcggca taattacgtg cggcagtaat ttccgaatcc
     4261 cccatcccat cgccaatcag caaaataata ttttttgcag gtttatcgct aagagaatca
     4321 cgcagagcgg cggtctgatc acccgttaag cggcgagcac cgccgggtgc agtaatatcg
     4381 ccctgagcag cccggttttc cagaacaggc atttctggtg tccgggcttt tgtcacaggg
     4441 gtaaacagta acggtaagag tgccagtgca atagtgcttt gtttcacttt attttctcca
     4501 tgtacaaata cattaaaaaa taaaaacaaa gcgactataa gtctcggccg tgacaacttt
     4561 atgacagctg ttgaaaagat taacttttta ctgacgagga tgcttcaata acttctttac
     4621 gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg gcatcgggct ttacctcgta
     4681 cagcgccccc tctacctgat caatcaaccg ctgttggtca ttttgcgcca tattgcgaag
     4741 cattgcagtg acgataatct ccaaagcttc tacctgcgca cacagttctt tcgactcttc
     4801 ttctttttgg gcaagcttaa ataacaactc agcaatgaga tttttcatgt ctgtatttcc
     4861 ttatccaaag tatggagaag ttaacattat gtttcacaac ttcatagtgt ctggaagtat
     4921 tatttttatg acaaaggaaa tactttgcgc aaatattttt tgtttgatta ccagcgaaac
     4981 gtttctctta tttaaataac tacccacaca aaatataggc ttttaattat caacct
//
LOCUS       DAPHDX010000219         4915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPHDX010000219 DAPHDX010000000
VERSION     DAPHDX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.222"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..964
                     /locus_tag="RH058_004311"
     CDS             11..964
                     /locus_tag="RH058_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV3139792.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYITQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1173..2138
                     /gene="lipA"
                     /locus_tag="RH058_004312"
     CDS             1173..2138
                     /gene="lipA"
                     /locus_tag="RH058_004312"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV3139793.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2239..2442)
                     /gene="tatE"
                     /locus_tag="RH058_004313"
     CDS             complement(2239..2442)
                     /gene="tatE"
                     /locus_tag="RH058_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV3139794.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2571..3359)
                     /locus_tag="RH058_004314"
     CDS             complement(2571..3359)
                     /locus_tag="RH058_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV3139795.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3452..3835
                     /gene="crcB"
                     /locus_tag="RH058_004315"
     CDS             3452..3835
                     /gene="crcB"
                     /locus_tag="RH058_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV3139796.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3889..4098)
                     /gene="cspE"
                     /locus_tag="RH058_004316"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3889..4098)
                     /gene="cspE"
                     /locus_tag="RH058_004316"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV3139797.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4273..4833)
                     /gene="pagP"
                     /locus_tag="RH058_004317"
     CDS             complement(4273..4833)
                     /gene="pagP"
                     /locus_tag="RH058_004317"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV3139798.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1313 a         1242 c         1100 g         1260 t
ORIGIN      
        1 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
       61 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
      121 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
      181 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
      241 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcac
      301 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
      361 aacgataact attgcgacca ctccctcggt cggagcactg gtccttcctg tcatctatcg
      421 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
      481 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
      541 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcgagggaaa
      601 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
      661 gttaccggaa gagcaaaatt tcagcggtct gcggcagaga gttcaagaaa tgtttccgga
      721 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
      781 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagccgct gctggccgct
      841 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
      901 caaattcagc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
      961 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
     1021 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
     1081 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
     1141 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
     1201 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
     1261 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
     1321 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
     1381 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
     1441 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
     1501 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
     1561 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc cagcactttg
     1621 cggattgcat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
     1681 cggatttccg tggtcgtatg gatcgtgctc tggatattct gactgcaacg ccaccagatg
     1741 tgttcaacca taacctggaa aacgtaccgc gtatttaccg tcaggtacgg cctggtgcag
     1801 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
     1861 ccaagtctgg tctgatggtg ggactgggtg aaaccaatga agaaattatt gaggtaatgc
     1921 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
     1981 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
     2041 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
     2101 acgccgattt gcaggcgaaa gggatggaag ttaagtaatc ctgtaataat tacttacatc
     2161 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
     2221 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
     2281 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
     2341 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
     2401 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
     2461 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
     2521 acacaatagc aatctgtaac aaggtgaatc aagcgccgag tagaaaaaca tcataataat
     2581 tgcggcggcg caaagcgacg gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca
     2641 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
     2701 acgccaaagg gatctataat ccggctttga ccgatatttt tgttcccgca ctcccccgcc
     2761 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
     2821 tctttgagcg gcccgcgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaat
     2881 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
     2941 atcccctcca cctcgagtaa cggagcgatt tcattaccag catcaacacg gcgtgattcc
     3001 tgaatggcaa atgcatcata gagatgcaat ttggcataac gggcgacgat gtttcctgcc
     3061 tgtagcgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga
     3121 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
     3181 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
     3241 tccggcagga cgaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
     3301 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcggc aaccagcatt
     3361 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
     3421 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
     3481 tggcggtggt acgggaagcg tggcgagatg gctgttaagt atgcgattta acccgctgca
     3541 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
     3601 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
     3661 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
     3721 gttacaagag ggccgctttg gctgggcgtt actgaacgtt ttcgtcaacc ttctggggtc
     3781 ttttgccatg acagcactgg ctttctggct gttttcggcc tcaaccgcac actaaagacc
     3841 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
     3901 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
     3961 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
     4021 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
     4081 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
     4141 ggttaccagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
     4201 aaagtcgcgt tatttttgat gttacttatt ttagctattg attttaaaga agttactaaa
     4261 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
     4321 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
     4381 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
     4441 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
     4501 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
     4561 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
     4621 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
     4681 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
     4741 catccactca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
     4801 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
     4861 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acata
//
LOCUS       DAPHDX010000220         4869 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPHDX010000220 DAPHDX010000000
VERSION     DAPHDX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.223"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            83..1999
                     /gene="mngA"
                     /locus_tag="RH058_004318"
     CDS             83..1999
                     /gene="mngA"
                     /locus_tag="RH058_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDV3139799.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGDTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVRVAEPIRHAEALMQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLLTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2017..4650
                     /gene="mngB"
                     /locus_tag="RH058_004319"
     CDS             2017..4650
                     /gene="mngB"
                     /locus_tag="RH058_004319"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDV3139800.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1196 a         1138 c         1343 g         1192 t
ORIGIN      
        1 atcaagtgaa attgatcaca taatggtatt gttttatcgg gcacactggc gcgactataa
       61 aaacgatcaa gtgaggatca tgatgaatct gacgactctg acccaccgcg atgcgttgtg
      121 tctgaatgcg cgctttacca gccgtgaaga ggccatccac gcgttgactc aacgtcttgc
      181 tgctctgggg aaaatttcca gtactgagca atttctggaa gaagtgtatc gccgtgaaag
      241 ccttggcccg accgccttag gtgaagggtt ggctgtgccg catggcaaaa ctgctgcggt
      301 aaaagaagcg gcgtttgcgg tcgccacact cagcgagccg cttcagtggg aaggcgttga
      361 tggcccggaa gcggttgatt tagtggtgct gctggcgatt ccccccaatg aagcgggtac
      421 tacgcatatg caactgctga cagcgctgac cacgcgcctg gcggatgatg agattcgggc
      481 gcgtatacag tcggcgacga cgcctgatga gttgctctcg gcgctggatg acaagggaga
      541 cacgcaacct tctgcctctt tttccaacgc gccaactatc gtctgcgtaa cggcctgtcc
      601 ggcgggtatt gctcacacct atatggctgc ggaatatctg gaaaaagccg gacgcaaact
      661 cggcgtaaat gtttacgttg aaaaacaagg cgctaacggc attgaagggc gtttaacggc
      721 ggatcaactc aatagtgcaa ccgcctgtat ttttgcggct gaagtcgcca tcaaggagag
      781 tgagcgtttt aatggcattc ccgcgctttc agtgcgtgtt gccgagccga ttcgccatgc
      841 agaagcgttg atgcaacaag cgcttaccct caagcgtagc gatgagacgc gtaccgtaca
      901 gcaagatacg caaccggtga aaagtgtcaa aacggagctg aaacaggcac tgttgagcgg
      961 aatctctttt gccgtaccgt tgattgtcgc ggggggcacg gtgctggcgg tcgcggtatt
     1021 actgtcgcaa atcttcgggc tacaagatct gtttaatgaa gaaaactcct ggctgtggat
     1081 gtaccgcaag ctgggcggcg ggctgctcgg aattttgatg gtaccggtgc tcgcggccta
     1141 taccgcctat tctctggcag ataaaccggc gttagcgcca ggctttgcgg ctggacttgc
     1201 cgccaacatg atcggctccg ggtttctcgg cgcggtcgtt ggcggattga tagccggtta
     1261 cttgatgcgc tgggtgaaaa atcacttgcg tcttagcagt aaattcaatg gattcctgac
     1321 tttttatctc tacccggtgc tcggtacgtt gggagcgggc agtctgatgc tgtttgtggt
     1381 gggagaacct gtcgcctgga tcaataactc gcttaccgcc tggctgaacg gtctgtcagg
     1441 aagtaacgcg ctgttgctgg gtgccattct cggttttatg tgttcctttg accttggagg
     1501 gccagtgaac aaagccgctt atgcattctg cctgggcgca atggcgaacg gcgtttacgg
     1561 cccgtatgcc attttcgcct ccgtcaaaat ggtttcggca tttaccgtaa ccgcttccac
     1621 gatgctcgca ccgcgcctgt ttaaagagtt tgaaattgag accgggaaat ccacctggct
     1681 gttagggctg gcaggtatta ccgaaggggc gatcccgatg gcgattgaag atccgctgcg
     1741 ggttattggt tcgtttgtgc tgggctctat ggtaacgggc gctattgtcg gtgcgatgaa
     1801 tatcggcctt ttgacacccg gtgccggcat tttctcgctc tttttacttc atgataatgg
     1861 cgcgggcggt gttatggcgg caattggctg gtttggtgcg gcattggtgg gggctgcaat
     1921 ctcgactgca attctcctga tctggcggcg tcacgcggtt aagcatggca actatctgac
     1981 tgatggcgta atgccataaa caaaaaggaa acgacgatga aagcggtatc tcgcgttcac
     2041 atcaccccgc atatgcattg ggatcgggag tggtatttca ccaccgaaga gtcacgcatt
     2101 ctgctggtca ataatatgga agagatcctg tgccgactgg aacaggacaa cgaatacaaa
     2161 tattacgtac tcgacgggca aacggcgatc ctcgaagatt atttcgcggt gaaaccggaa
     2221 aacaaagacc gcgtgaagaa acaggtagaa gccggtaagt tgattatcgg cccctggtat
     2281 acccagaccg ataccacgat tgtttctgcg gaatccatcg tccgtaatct gatgtacgga
     2341 atgcgtgact gcctcgcgtt tggcgagccg atgaaaatag gttatttacc ggattccttt
     2401 ggcatgtccg ggcaactgcc gcatatctac aatggatttg gcattacccg cactatgttc
     2461 tggcgcggat gttcggagcg ccacggtact gataaaaccg agtttttatg gcaaagcagt
     2521 gacggtagcg aagtgacggc gcaggtgctg ccgttgggtt atgccatcgg taagtactta
     2581 cctgccgacg aaaacggatt acgcaaacgc ctcgacagtt attttgacgt gctggaaaaa
     2641 gcgtctgtaa ccaaagagat tttattgccg aatggtcatg accagatgcc attgcagcaa
     2701 aatatcttcg aagtgatgga gaaacttcgc gaaatttatc ctcaacgaaa gtttgtgatg
     2761 agccgctttg aagaggtatt cgagcagatc gaagcgcagc gtgaaagtct ggccaccctg
     2821 aaaggtgaat ttatcgatgg caaatatatg cgcgtacatc gcaccatcgg ttccacgcgc
     2881 atggatatca aaattgccca cgcacgtatt gaaaataaga ttgttaatct gctggaaccg
     2941 ctggcaacgc tggcctggac gttgggtttt gaataccacc acggcttgct ggagaaaatg
     3001 tggaaagaga tcttaaaaaa tcatgcccac gacagtatcg gttgttgttg cagtgacaaa
     3061 gttcatcgcg aaattgtcgc ccgcttcgaa ctggctgaag acatggcgga taatctgctg
     3121 cgtttctaca tgcgcaaaat tgccgataac atgccacaga gtgatgccga caaactcgtg
     3181 ctgtttaacc tgatgccctg gccgcgggaa gaagttatta acaccactgt gcggctgcgc
     3241 gccagccagt ttaatctacg agacgatcgc ggtcagcctg taccgtattt tattcgccat
     3301 gcccgtgaga tcgatccagg cctaatcgat cggcaaatag ttcattacgg taattacgat
     3361 ccctttatgg agtttgatat acagatcaac cagattgtcc cttctatggg ctatcgcacg
     3421 ctttatatcg aagcgaatca gcctggcaac gtgattgcgg caaaaagtga cgctgaaggg
     3481 atactggaaa atgctttctg gcaaattgcg ctcaatgagg atggaacgct acaactggta
     3541 gataaagata gcggtgtgcg ctatgaccgg gtgttgcaaa ttgaagaaag ctctgatgat
     3601 ggtgatgaat atgactattc acccgcaaaa gaagagtggg taatgacttc tgcaacagcg
     3661 aaaccgcaat gcgagattac tcatgaggcc tggcagagca gggctgttat ccgctatgac
     3721 atggcggtgc cgctcaattt gttggaacga agcgtccggc aatccacggg cagagtaggg
     3781 gtggagatgg ttgtcactct gagtcataac agcagacgta ttgatgtgga tatcaacctt
     3841 gataaccagg ctgacgatca tcgccttcgc gtcctgatcc ctacatcttt taacaccgat
     3901 agtgttctgg cagataccca gtttggttcg ctaacgcgcc ccgtgaacga cagtgcaatg
     3961 aacaactggc agcaagaagg ctggaaagaa gcgccggttc cggtatggaa tatgctcaac
     4021 tatgcagcct tacaggaagg gcgtaacggc atggctgtct ttagcgaagg gctacgtgaa
     4081 tttgaagtca tcggtgaaga gaagaaaacc tttgccatta cgttgctgcg tggcgtgggc
     4141 ttactgggca aagaagatct gtttttaagg cctgggcggc cttcgggaat taaaatgcca
     4201 gtcccggact cacaactacg tggtctgctt tcttgtcgcc taagtttatt gagttatacc
     4261 ggtacgccaa ccgccgctgg tgtagctcag caggcgcgag catggctgac tccagtacag
     4321 tgttacaaca aaatcccatg ggatgcgatg aagctcaaca aagccggatt caacgtgccg
     4381 gaaagttata gtttgttgaa aatgccccca gtgggatgcc tgataagcgc acttaagaaa
     4441 gctgaagacc gacaagaagt gattttacgg ctgtttaatc cggctgaatc agcaacctgt
     4501 gatgcgactg ttgctttcag tcgcgaggtg atttcttgct cagaaacgat gatggatgaa
     4561 cacattacca ccgaggaaaa tcaaggttca aatctatcgg ggcctttttt acccggccag
     4621 tcacggacgt tcagttaccg gcttgcctga atagcaatca aaccgaagcc acatatgcgc
     4681 ggccagattg ttgacaaagg gcgctttgtt catgccggat acggcatgaa cgctttattc
     4741 ggtctacaaa agcaggcaaa ttcaatatat tgcagagatg atgtagacac tggcaagcgt
     4801 agcgcatcag gtaattttgc gtttatcttc actctcaagc cacgtatatg tggctttatt
     4861 tttaacaaa
//
LOCUS       DAPHDX010000221         4851 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPHDX010000221 DAPHDX010000000
VERSION     DAPHDX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.224"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(67..828)
                     /gene="kduD"
                     /locus_tag="RH058_004320"
     CDS             complement(67..828)
                     /gene="kduD"
                     /locus_tag="RH058_004320"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV3139801.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(858..1694)
                     /gene="kduI"
                     /locus_tag="RH058_004321"
     CDS             complement(858..1694)
                     /gene="kduI"
                     /locus_tag="RH058_004321"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV3139802.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1981..3162)
                     /locus_tag="RH058_004322"
     CDS             complement(1981..3162)
                     /locus_tag="RH058_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV3139803.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTSVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3417..4646
                     /locus_tag="RH058_004323"
     CDS             3417..4646
                     /locus_tag="RH058_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3139804.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTHLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1263 a         1236 c         1061 g         1291 t
ORIGIN      
        1 ttatgatgat ctacagatat aaaaaaccct gccatgcggc agggtcataa aagtaagaag
       61 aatgaattaa cgcgccagcc aaccgccatc cacggcaatg gtataaccat tcacataatc
      121 tgaagcgctg gaggcaagga acactaccgg ccccatcagg tcactcggca gtccccaacg
      181 gccagctgga atgcggtcga ggatttccgc gctacgttgt tcatctgccc gtagttgttg
      241 agtattgttg gtcgccatgt aacccggggc tatcgcatta acattaatgt tgtgtttagc
      301 ccattcgttc gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga
      361 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
      421 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
      481 attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg
      541 aatcaatccg gcgttattca ccaggatatc aatatgacca aactccgcta ccgcgcgatc
      601 cagcagtgct ggaataccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
      661 ccccagcgcc gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat
      721 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc
      781 agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta
      841 ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaag
      901 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
      961 tgaatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt
     1021 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
     1081 taaacttcca tccggcgctc gtgagtgtga cacggcatgg tgttccacaa gttacctgga
     1141 gccagctccg tcagccccat actcaattgg caggtttcca gcacatccgg gacaaaatat
     1201 ttgttaattg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
     1261 tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
     1321 tttgccggag tggcggtatc aatactggca aagacaactt cttttgcacc tttacccaca
     1381 tacagggcgt cgcggtgacc gatttcatag cattggccat cgacagtaat cgtaccggca
     1441 ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt
     1501 ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata
     1561 attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac ctcttcaacc
     1621 aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcact gtggatgctc
     1681 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt
     1741 gaaacactgt ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat
     1801 taaaaataaa acaatgtttc atttttaagt taaggattaa aaaaagtgca gtgtcgatca
     1861 cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag
     1921 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag
     1981 ctactcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc
     2041 cagtcctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca
     2101 accggaagcg cctatcgggt gaccaagtgc gatcgcacca ccattgacat tgacccgacg
     2161 ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
     2221 ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc
     2281 atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcgaat
     2341 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc
     2401 agctgcgcca tcgtttatgg atgatgcatt acccgctgtc acagaaccga gactatcaaa
     2461 tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt
     2521 atcaacaacc aacgtctgac cgttactttg ggtcattacc gggacgatct catctttaaa
     2581 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
     2641 caattgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
     2701 atcattgaag gcatcccata acccatcatg cacaagactg tcaaccagct ggctattgcc
     2761 aagctgtgca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
     2821 gccaccggcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
     2881 tgctttaagt ccggaaccgc agacgtcatt aatcgtgatt gcagaaacgc tattaggcag
     2941 accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg
     3001 accaagaatt acttcatcca ccgcatatgc aggaacgctg gtacgttcta ttaacgcttt
     3061 cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccgc gaaagcagcc
     3121 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
     3181 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat
     3241 ttttatcaca aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt
     3301 tgcaaccact aacccaccaa tagaggggta gatagggcgt taatctccca tacttaacct
     3361 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
     3421 ctaatatttg gtctaaagaa gaaactctgt ggagtttcgc gctctacggc acagccgttg
     3481 gtgcaggcac gctcttcctt cctattcagt taggttcggc aggggctgtg gtcctgttta
     3541 ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca
     3601 tcctctcatc gaaaacatca gcaggtgaag ggataacggg cgcagtaaca cactactatg
     3661 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
     3721 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
     3781 ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaatcctg aatctcattt
     3841 ttctgatggg acgccatgca accattcggg taatgggttt tttggtattc ccattgattg
     3901 cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacga
     3961 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
     4021 ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
     4081 atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
     4141 gcataagtgt actgttcttt gtctttagct gcctgctttc tattccacct tcgtatattg
     4201 aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc
     4261 cagcatggct gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
     4321 tgggtacgta ctttggcgtt attgaaggtg ccacggaggt cgtcaaaaca acactacagc
     4381 aggttggtgt aaagaaaagt cgtgcattta accgcgcact atcaattatg ttggtatcgc
     4441 tgatcacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca
     4501 gcggcccgct cattgccatg atacttttca tcatgcctac gttgtcaacg catctgatcc
     4561 cggcgcttaa accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt
     4621 gcgtatcggt aatgttcttt agctgatatc cattgtgaat ggttcaaaat tgctacaatt
     4681 ttgaaccatt ccagttttca taatgttcat tcgttattat tccgcactat cacttccctc
     4741 atcatatcct agtattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa
     4801 gactatttaa gatgcataac ttagattcgc aagatatctt ccggtcattc a
//
LOCUS       DAPHDX010000222         4804 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPHDX010000222 DAPHDX010000000
VERSION     DAPHDX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.225"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..1091
                     /gene="hemB"
                     /locus_tag="RH058_004324"
     CDS             117..1091
                     /gene="hemB"
                     /locus_tag="RH058_004324"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV3139805.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1197..2048)
                     /gene="tauD"
                     /locus_tag="RH058_004325"
     CDS             complement(1197..2048)
                     /gene="tauD"
                     /locus_tag="RH058_004325"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV3139806.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2045..2872)
                     /gene="tauC"
                     /locus_tag="RH058_004326"
     CDS             complement(2045..2872)
                     /gene="tauC"
                     /locus_tag="RH058_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV3139807.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2869..3636)
                     /gene="tauB"
                     /locus_tag="RH058_004327"
     CDS             complement(2869..3636)
                     /gene="tauB"
                     /locus_tag="RH058_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV3139808.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3649..4611)
                     /gene="tauA"
                     /locus_tag="RH058_004328"
     CDS             complement(3649..4611)
                     /gene="tauA"
                     /locus_tag="RH058_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3139809.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1103 a         1391 c         1233 g         1077 t
ORIGIN      
        1 atgtcctggt ctctctttca ttctcttctt gtcaacatca cgacaacttt cgtaaaacac
       61 tcctgccccg cttcaggtat actgtgcacc ttaattccac aaacatcagg cagaccatga
      121 cagacttaat ccaacgccct cgtcgcctgc gcaaatctcc tgcgctgcgc gctatgtttg
      181 aagagacaac acttagcctt aacgacctgg tgttgccgat ctttgttgaa gaagaaattg
      241 acgactacaa agccgttgaa gccatgccag gcgtgatgcg cattccagag aaacatctgg
      301 cacgcgaaat tgaacgcatc gccaacgccg gtatccgttc cgtaatgact tttggcatct
      361 ctcaccatac cgatgaaacc ggcagcgacg cctggcggga agatggactg gtggcgcgta
      421 tgtcgcgcat ctgcaagcag actgtgccag aaatgatcgt catgtcagac acttgtttct
      481 gtgaatacac ttctcacggt cactgcggtg tgctgtgcga gcatggcgtc gacaacgacg
      541 cgactctgga aaatttaggc aagcaagccg tggttgcagc tgctgcaggc gcagacttca
      601 tcgccccttc cgccgcgatg gacggccagg tacaggcgat tcgtcaggcg ctggacgctg
      661 caggctttaa agatacggcg attatgtcgt attcgaccaa gttcgcctct tccttttatg
      721 gtccgttccg tgaagctgcc ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga
      781 acccaatgaa ccgtcgcgag gcgattcgtg aatcactgct ggatgaagcc cagggcgcag
      841 actgcctgat ggttaaacct gctggagcat acctcgacat cgtgcgtgag ctgcgtgaac
      901 gtactgaatt gccgattggc gcgtatcagg tgagcggtga gtacgcgatg attaagttcg
      961 ccgcgctggc gggtgctata gatgaagaga aagtcgtgct cgaaagctta ggttcaatta
     1021 agcgtgcggg tgcggatctg attttcagct actttgcgct ggatttggct gagaagaaga
     1081 ttctgcgtta atttttttcc tctcagaaag ggggagttaa cctgttgaaa gtacggtgaa
     1141 cgccccctca ccctaaccct ctccccaagg gggcgaggga accgtccgca ctcgtatcac
     1201 cccgcccgat aaaacggttt atccccaaga atcgtcgccc gatgcataat ccgccgctgt
     1261 ggcaggtaat cggcattggc atagtgctga gtcacgcggt tatcccaaat cgcaatatca
     1321 ttcggttgcc agcgccaacg cacctgaaac tccggtttag tgatatgggc gaataaaaag
     1381 cctaacaagg cttcgctctc tttctcgctg acatcgacaa ttcgcgtagt aaagccttca
     1441 ttcacaaaca gcgcctgttt accgctcacc ggatgcgttc gcaccaccgg atgtagcaac
     1501 ggaggatttt tcgcgaccgc ctcgcgccag cgttgatgtt cctcctcggt tttgcggtat
     1561 ttgtattccg ggaacgattt acggaaatca tgctccgcac gcagcccgct cagcagctgg
     1621 cggaagggaa cagagagcgc ctcataggcc gcaataccgc tggtccagag cgtatcaccg
     1681 ccggtcgaag gtaactcttt agctgccaga atcgcccctg cgggtggcgt ttcaataaat
     1741 gtcacatcgg tatgccagtt gtcgttatcc ggcggattat cgttatgggt atccagcacg
     1801 atgatctcgt caaccccttc ggcatgaggg taaacagggt gaatatgcag ttcgccaaaa
     1861 cgctgggcca gcgcgcgttg ctgctgcggc gtaatagttt gatcgcgcag aaacaccacc
     1921 tgatggcgca gcaccgcatg gtaaagctgt tcaaactgat tatcgcttaa cgggcgcgtc
     1981 aggtcggcac ccgaaatttg tgcgccgata tacggcccca gcggggtaat gctcagacgt
     2041 tcactcattg tacttctcca tgccagggcg tcagacggcg ctgtaacgcg cgcaaaccta
     2101 gttctaaaag aaaggcgata atcgcaatca ccgcgattcc cgccagcacc acgtcagtcg
     2161 cgagaaattc acccgctgac tgaaccataa atcctaaacc gcgcgtcgcg gcaatcagct
     2221 ccgccgctac cagcgtagac cagcccacac ccagaccaat gcgtaatccg gtgaggattt
     2281 ccggcagcgc gccgggcaaa atgacaaacc acagcacctg cgcacggctg gcacccagcg
     2341 actgggcggc acgaatgcga acctgctgcg cgcttttcac ccccgccagc gccgacatcg
     2401 ccaccggtgc aaaaatcgct aaatagatca gtaagatctt cgaggtttca ccaataccaa
     2461 accagatcac catcagcggc aaataagcca gcggcggcac gggacgataa agttcgatta
     2521 ccggatccag aatgccgcgc accgtcgggc ttagccccat ggcgatcccg accggaatac
     2581 cgatcaccac cgccgcgagt agcgccagca caatgcgcgt caggctggct gccagatgct
     2641 gccacagcgt ggcgtccata aatccctgcg gtccggcaat ggtgagcagt tttgccagta
     2701 cttgttgcgg cggcggcaaa aacagcgggc taatcagttg cagcgccgcc accgtccacc
     2761 atacagtgag taaaaccgct aacgtgccaa tgcttaaggt cacctgacgc gagagcggcc
     2821 agcgccattt cagccgccgc gaatgcagtt tttcattaat gagcacactc atgagaacgc
     2881 ctcccgttgc tcaaatacgc ggcttaaaac atattcgcgc atggcgatga attgtggatc
     2941 ggacttgatg ctgcggctcg actctcccgc aacaaagcgg cgagcaaagt tgagcggcag
     3001 ccgctccagc acacggccag ggccggatga aagcagaacc agttcagtcg ccataaacac
     3061 cgcttcttct atatcgtggg taatcaacag cacctgcttg cccgtctcct gccagagttt
     3121 cagcagcaga gtttgcatct ggtcgcgggt gaaggcgtcc agcgcaccaa acggttcgtc
     3181 gagtaataac agctggggat tcgccgccag cgcgcgggca atccccaccc gctgacgttg
     3241 accaccggaa agctgccaga tatagcgttt ttctgcgcct tccagcccca cttttttcag
     3301 catctggtgc gcgatttcca gtcgctgcat tttctctata cctgccaatt gcaggccgaa
     3361 cgccacgttg tcctgtacat tgcgccacgg tagtagccct tcattctgaa aaaccacccc
     3421 gcgctctgct cccggtccct caatacgctt acccgccagt tgaatgctgc catgctgata
     3481 aggcacaaaa ccggcaatca gattcagcag ggtggtttta ccgcagccgg acggccccag
     3541 caccaccagt agctcgccgc tttccagcgt caggttgata tcttccagta ctggtttgcc
     3601 gccataatcg gcgtaaagat gagagatttg cagcatctgc gcctcctttt attgcacgaa
     3661 gcgcgaggta acgtactggc tgtaatcatt cgctacagcc gggaccttgc cctgctcttt
     3721 caaaaactgc gcggtgtcga tgatcgcttt gttcaccggt ccggtcagtt ctaccgtttg
     3781 ttgctgcggc gtcagatagg tattcccctt caccagcccc ggaacgtcac cttccggcac
     3841 gccgcttaaa cgcgccagtt tgctgatgtt ttccggctgt ttcagccacg cgtctgggtt
     3901 agcaatgtac ggttgctgag catcgatggc gcttttagcg aacgctttca cgacctcagg
     3961 atgtttctcg gcaaaatctt tgcgcaccac ccagacgtcc agcgttggcg caccccactg
     4021 cccgacctgt tcagaatcgg tcaacacctt gccgtctttt tccagggcgt taaccgccgg
     4081 tgcccagaca taagcaccat caatatcacc ccgctgccat gcggcgataa tcgcgggcgg
     4141 ctgcaggttc acaatctcca cttgcccggg tttaatcccc cagtgtttca gcgccgccag
     4201 caggctgtag tgggtggtgg agataaacgg tacggcaatg cgtttgccaa tcaaatcttc
     4261 gggtttgctg atagttttct ttaccaccag cgcttcggag ttacccagtt ttgacgccag
     4321 caagaagact tcaatcggca cctgttggct ggctgcaacc gctaatgggc tggaaccgag
     4381 gttgccgatt tgcacgtcgc cagaagccag cgcccgcacg atgctggctc cgctgtcaaa
     4441 cttacgccag tccacggttg ctccgctttc tttagcaaag gtgttgtcgg cctgagccac
     4501 tttcgccggt tcggctgagg tttgatacgc cacggtgacg ttcaccgcct gtgcctgaaa
     4561 agcgatgaat gccagtgcgg caagaagtgt gttacgcgat gaaattgcca tgattgtctg
     4621 ctcccctgtc ttgttatgtg agcagtattc aggaataaaa acattcatta aaagaattag
     4681 tcgttatcgc acagatgatt ttattcttag caaaaaaacg gcaaaggaaa gattaaatat
     4741 gcaattactc cgttctaaca attgggaaaa ttactacact tcattttagt gatgcataaa
     4801 caac
//
LOCUS       DAPHDX010000223         4799 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPHDX010000223 DAPHDX010000000
VERSION     DAPHDX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.226"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..564
                     /locus_tag="RH058_004329"
     CDS             <1..564
                     /locus_tag="RH058_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3139810.1"
                     /translation="IVYPPILSNGMFNFIVMQTLFYLPFFILGALAFIFPHLKALFTT
                     PSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNF
                     QSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEI
                     HLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(572..2053)
                     /gene="clsC"
                     /locus_tag="RH058_004330"
     CDS             complement(572..2053)
                     /gene="clsC"
                     /locus_tag="RH058_004330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV3139811.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(1995..2528)
                     /gene="ymdB"
                     /locus_tag="RH058_004331"
     CDS             complement(1995..2528)
                     /gene="ymdB"
                     /locus_tag="RH058_004331"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV3139812.1"
                     /translation="MKTCIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2623..2934)
                     /locus_tag="RH058_004332"
     CDS             complement(2623..2934)
                     /locus_tag="RH058_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3139813.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3055..3387)
                     /gene="csgC"
                     /locus_tag="RH058_004333"
     CDS             complement(3055..3387)
                     /gene="csgC"
                     /locus_tag="RH058_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV3139814.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3446..3901)
                     /gene="csgA"
                     /locus_tag="RH058_004334"
     CDS             complement(3446..3901)
                     /gene="csgA"
                     /locus_tag="RH058_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV3139815.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3942..4397)
                     /gene="csgB"
                     /locus_tag="RH058_004335"
     CDS             complement(3942..4397)
                     /gene="csgB"
                     /locus_tag="RH058_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV3139816.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1215 a         1197 c         1057 g         1330 t
ORIGIN      
        1 attgtgtatc cgcccattct gagtaatggc atgttcaatt ttattgtcat gcaaacgctg
       61 ttttatttgc cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc
      121 ttgtttacca cgccgtctcg tggctgtacc cttgcagcag cattggcgtt tgtcgcttat
      181 ttactcaacc agcgctatgg cagtggcgat gcctggatgt acgaaaccga gtcggtgatc
      241 accatggtcc tcggtctgtg gatggtgaat gtggtcttct cctttggcca ccgtttgctt
      301 aacttccagt cagcgcgggt gacttatttt gttaacgcat cgctgtttat ctatctggtt
      361 caccacccgt taacgctgtt tttcggcgca tacattacac cgcacatcac ctccaactgg
      421 cttggttttc tctgtggcct gatatttgta gtagggattg cgataattct gtatgaaatt
      481 catttgcgca tcccgttact gaagtttttg ttctctggta aaccggttgt taagcgtgag
      541 aacgataaag caccagcccg ttaagccaca tttacaataa ccattccacg ggcaatatcg
      601 acgccagtct gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaataa
      661 tctctttctt tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct
      721 gccaggccgc atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt
      781 cgctctctat cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga
      841 aagagccgat aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggctc
      901 cggaattacc ggttatgccg cgatcgtgta acgtactact ttgttcacgc gtcggcttga
      961 gttcatataa ttccacgcca tagcggagca attttttgcg ccagcgcgcg tatccggcat
     1021 ggacgacagc aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt
     1081 ttctcaccat ccgtaagagt tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga
     1141 taatatcgat gcgttcactg ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg
     1201 aatgccgttt tgccttgccc tcccctttcg ccggatcatc acttaataaa cgtgttttcg
     1261 cccagataag cggcaacgtt ccatcaacca gatgatttat aaacggactg gattccattt
     1321 tgcgtaagta acgatgcgtc atagcatcgt tatgccagga ggcgggtaac tcgatgcgat
     1381 ccgccatttc accttccggg acatccagca cttgctgtaa gggtgaaacc gatttgcaat
     1441 accagtagcg ggcgaaatca tcggcaacgt cctctaccac gggccctatt gccataacat
     1501 ctaaatccga aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgcc
     1561 cccctaccag cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga
     1621 gacgggaaaa gtcggtgata taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa
     1681 aaagccggac ttcaatgcgg ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg
     1741 gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca
     1801 gggcggaaaa cagtaaccga cccgacatat cgtcctgcca gatgtaatac tgaacatcca
     1861 gcgtatgctc tgccatttcg gccagacgat accgggcggc aaacgcatcc agacttttct
     1921 ccagagggaa aagaccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg
     1981 ccagccgggg caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg
     2041 ttttcttcat cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat
     2101 tctgaaacgg ttttaactgc gatttcagcc gctgccgcac gagggtaacc ataaacccca
     2161 gtactgattg caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgtaggcta
     2221 ttgagatagg catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc
     2281 ccgacggtgt gcactacggc tttagcggga agagcgcctg caagcgtaat aacggcatgc
     2341 cccgtagggc aatcgccctg ctgttgcctg acttttaaac aggcatccag cagggccgga
     2401 cccgctgcgc gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc
     2461 gcattcacaa tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacac
     2521 gttttcataa cgcctcctta caccttcttg tttcttaagt gtacagcagg ctttttggtt
     2581 aaatcgcatt tttctgctca gcaacgtgcg ttgttcagaa acttagttat attcgatgtt
     2641 cattacagcc agctttttct gtttatcaag atactgcact tttactgagg ctatttgcgc
     2701 gtttttaaca ttaccgctca ttaacgcttt gctggaatag gttttgcccg gaacagaacc
     2761 attctgtggg cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc
     2821 cacaatttgg ccataaaaat ggatcacccc cccttgcgcg gtgcttcctg cgatggcaat
     2881 aaaaggaaaa agcaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc
     2941 tatttcctca atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg
     3001 attcgcagca gaccattctc tccagattca tcttatgctc gatatttcaa caaattaaga
     3061 cttttctgaa gagggcggcc attgttgtga taaatgaagt gactgtccat cagaaacagt
     3121 aacaactatt ttcacccgat cttccgggga aatatttaaa ctcaacttcg tcaaagcaat
     3181 aggttgatta gcaggcaatg agagggtctt ttcttgcttc gtctgacttt gccctgaact
     3241 gccttcgcgc agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc
     3301 aggaataatg gtatacacat ccccttgctg ggtcgtatta aaggttatct ggctggaaag
     3361 tgccgcaagg agtaataacg cattcatatt cttctcccga aaaaaaacag ggcttgcgcc
     3421 ctgtttctgt aatacaaatg atgtattagt actgatgagc ggtcgcgttg ttaccaaagc
     3481 caacctgagt cacgttgacg gaggagttag atgcagtctg gtcaactgca gcaccgttgc
     3541 caccaccgaa ctgtttaaca gtcatttcag aatttttgcc gttccactga tcaagagtgg
     3601 cgctgttacc gaagccacgt tgggtcagat cgattgagct gtcatctgag ccctgaccta
     3661 catctgcacc attaccaccg ccatgctggg taatagtcaa gtcagagtta cgggcatcag
     3721 tttgcagagc aagtgcagag ttaccgccac cgtactggta aatgttcagc tctgaatttg
     3781 ggccgctatt attaccgcca ccaccgtggt taccgccgcc gccgtactga ggaacaacac
     3841 ctgccagagc gctaccggag aatacgattg ctgcaattgc tgctactttt aaaagtttca
     3901 tgtaaaaccc ccatcggatt gatttaaaag tcgaatggaa attaacgttg tgtcacgcga
     3961 atagccattt gcgactgtct ctgcactaca attgccgttt tttgagtacc atactgtgta
     4021 atatttgctt tattaccaga acctttctgg ataatcatcg cagtattacc ataagcacct
     4081 tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag gttataatct
     4141 cctgtctggt caatctttgc ccggttgcta ctaccttctt gcgcaacaac cgccaaaagt
     4201 tttgagcctc cctgccgtaa ctgagcacta ttattcgtcc cagcttgacc aattatggct
     4261 gcctgattaa atgaagactt actcaattca tttaccgcga agttatattc tgaattagct
     4321 aaatcataac ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa catcataaat
     4381 aacaatttgt ttttcatgtt gtcaccctgg acctggtcgt acatttaaga aattaaatca
     4441 tttcaacttg gttgttaacg caacctgtat tttgttaacg ctgcgttacg atggaaagta
     4501 tgtctgcgga aatattttta ataactcacc cgcgcgttgt atttttcttt ttttgttcat
     4561 accaaagtat taaaacccac gtaaatacgc tgttatctac gcaaaaaata ttttgttttc
     4621 ttttaaacct ccgttttccg ctatcaaaaa tcaccagaca gtcatttttc ttgcccgtcg
     4681 ctgattgctg cgatatgtct tgcgcacaag ccgtgacaaa cctcgtcttt ttgcagtaaa
     4741 aaattgtcca cggaggtgcg gagaaaaaac aagaacgttt tacatgacga aaggactac
//
LOCUS       DAPHDX010000224         4574 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPHDX010000224 DAPHDX010000000
VERSION     DAPHDX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.227"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH058_004336"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH058_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3139817.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="RH058_004337"
     tRNA            complement(324..399)
                     /locus_tag="RH058_004337"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="RH058_004338"
     tRNA            complement(406..480)
                     /locus_tag="RH058_004338"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="RH058_004339"
     tRNA            complement(597..681)
                     /locus_tag="RH058_004339"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="RH058_004340"
     tRNA            complement(690..765)
                     /locus_tag="RH058_004340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="RH058_004341"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="RH058_004341"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV3139818.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="RH058_004342"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="RH058_004342"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV3139819.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="RH058_004343"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="RH058_004343"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV3139820.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4399..4514)
                     /gene="rrf"
                     /locus_tag="RH058_004344"
     rRNA            complement(4399..4514)
                     /gene="rrf"
                     /locus_tag="RH058_004344"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1157 a         1154 c         1031 g         1232 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcttgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca cccaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
     4441 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
     4501 ctacggccgc caggcaaatt ctgttttatc agactgcttc tgcgttctga tttaatctgt
     4561 atcaggctga aaat
//
LOCUS       DAPHDX010000225         4509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPHDX010000225 DAPHDX010000000
VERSION     DAPHDX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.228"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            250..822
                     /locus_tag="RH058_004345"
     CDS             250..822
                     /locus_tag="RH058_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV3139821.1"
                     /translation="MASATSENTSSTKNEAQTVLSERSLKAEQRGMEIMDDMQYARLA
                     LFSGDTGAADKLINRAEDLLKSDDKTWETVVRKDKKTPQEGDVYIIINSSLMLSEDFT
                     VTPEKQKAIDEANKKLDKGDKKGAIDVLHLAGIDISETQWLMPLKQTQKKVNSAQKLM
                     SEGKYYEANLALKGAEDGVITDTVSLNEGD"
     gene            978..1712
                     /locus_tag="RH058_004346"
     CDS             978..1712
                     /locus_tag="RH058_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3139822.1"
                     /translation="MFPYCFAIIVRPLCASFYNEELDFARNEILFLKRNDIQYITDAC
                     EGCVRFIDIPCHIISDYLYIRLRDADALCITDRKNSFFRKKAYGTFLNSLMSLAEQQK
                     NTNLYESVLFVLLDYLNDKNQLLPVLSGGLNNYSLRVEAIVAAEPAKKWYLSDVASAL
                     CISASQLKRKLHSEGAGFSRIITEVRMKKAITLMKCGNANIFIVARECGYSSLSYFIL
                     SFRKYYSVTPCQWLKQYGIKDTTGDG"
     gene            1955..3184
                     /locus_tag="RH058_004347"
     CDS             1955..3184
                     /locus_tag="RH058_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3139823.1"
                     /translation="MSYPVQKKIKTGENVLRAAVERINWVFDTFAEICLSFSGGKDST
                     VLFHLVADIARRKKRRFSVLFIDWEAQYLCTVRHVQKMRELYMDVTESFYWVALPLTT
                     VNGVSQFQPEWICWESGVEWVRQPPDYAVTDPNFFPFYQYAMTFEEFIPAFSSWMARS
                     KRGLAVLTGVRADESLNRFMGLASQHKLRYADDKPWTTASQDGFYYTMYPLYDWKTRD
                     IWIYNAKTHAIYNPLYDLMFRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMC
                     ARVSGAASGALYANESGAYFALRKRISKPDHHTWRSYAMFLLNVMPEKTAEHYRNKIA
                     VYLHWYQTHGYPDDIPDEQDNDLGSRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHY
                     ERYLQRMKERRKEWGIL"
     gene            3169..3807
                     /locus_tag="RH058_004348"
     CDS             3169..3807
                     /locus_tag="RH058_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV3139824.1"
                     /translation="MGDPLTPEILTVMIRQYFCQHLSEEETINALNHLRRVLHEISPF
                     AREPVDCVLWVKSDEVIANDYNPNVMAPGEKKLLKQSLEQDGFTQPVVVSEEKDHYLV
                     VDGYHRQLLGRGAGTEKRLRGWLPVTCINPERKGQATRMAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWSDERIGTELGMDQDEVLRLKQISGLTELFQDEDFSPAWTVR"
     gene            3792..4148
                     /locus_tag="RH058_004349"
     CDS             3792..4148
                     /locus_tag="RH058_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV3139825.1"
                     /translation="MDGSLIQLNKILVDEFLSTQKRALEAVDDLIALKLEAAGCWRRA
                     SARWLVVMGAGDITDAQREWLLRRRAYCMAQTTSHVLHEKMNIRGVAKAADETLKRMG
                     IADLSEEMFRKRPSYY"
BASE COUNT         1274 a          843 c         1133 g         1259 t
ORIGIN      
        1 gggggtaagt ggtcagattt cttatatcag ttgcagaaca taaaatatgc atgccggata
       61 ggggagtata aatgtccggg agggggacat ccttgtccgg cttcctgagt atatgactaa
      121 tcgtcagggg gaaaatcata ttatgtttat tatcaggtat gattctttct gcctgatgtc
      181 ggagtaaaaa atatgaaaca tatctttaat gctgtagcaa tcgctctgtc cctgacctct
      241 gctgtcgcaa tggcctcagc aacttcagaa aacacttcat ccacaaaaaa tgaggctcag
      301 acagttttat cagaaaggag tctgaaggca gaacagcgtg gtatggaaat tatggatgac
      361 atgcagtatg cacgtcttgc cttattcagt ggtgacacag gagcagcaga taagctgata
      421 aaccgggctg aagatttgct taaatctgat gataaaacat gggaaacagt tgtccgcaag
      481 gataaaaaaa caccacagga aggcgatgtc tacattatta ttaacagcag cttaatgttg
      541 tctgaagact ttactgtcac cccggaaaaa cagaaagcta ttgatgaggc caataaaaaa
      601 ctggataaag gtgacaaaaa aggagcaatt gacgtactgc atctcgccgg aattgatatt
      661 tcagaaacgc agtggctgat gccattaaaa cagacgcaga aaaaggttaa cagtgcgcaa
      721 aaattaatga gcgaagggaa atactacgaa gctaatcttg cactgaaagg tgcagaagat
      781 ggtgtgatta cggatactgt cagcctgaat gaaggtgact gataacattt atatgcgcgt
      841 ataaccacgg tgaccggact gtattttgtc cggttacttt aataataaaa ccgaacttct
      901 taatataatg tcaggaagta agtctgtcag ctataataag aaaaataccg gatatttcta
      961 aataacacct gtactgtatg tttccgtatt gttttgccat tatagtgagg ccactctgtg
     1021 cttcatttta caatgaagag cttgattttg cccggaatga aattctcttt cttaaaagaa
     1081 atgacattca gtatattacg gatgcctgtg aaggatgtgt gaggtttatt gatataccct
     1141 gtcatatcat cagcgactat ctgtatatca gactgaggga tgccgatgcg ttatgcatca
     1201 ctgacaggaa aaattctttt ttcagaaaga aagcgtatgg tacctttctg aactcgctga
     1261 tgtcacttgc ggaacaacag aaaaatacta acctttatga gtcagtatta tttgtgctgc
     1321 tggattatct taatgataaa aaccagttgc tgcctgtatt gtccggtggg ctgaataact
     1381 actcgttacg tgttgaagct attgttgccg cagagccggc aaagaaatgg tatctgtcag
     1441 atgttgcatc agcgttatgt ataagtgcca gtcagcttaa aagaaaactg cacagtgaag
     1501 gcgcaggatt cagccggata atcacagaag tacgtatgaa aaaggcaata actctgatga
     1561 agtgcgggaa tgctaacatt tttattgtcg ccagagagtg cggatatagc agtctgtcgt
     1621 attttatttt aagtttcaga aaatactact cagtcactcc ctgtcagtgg ttaaaacagt
     1681 atggtattaa ggacacaact ggtgacgggt aaaccctgat tcacataaac cggttttctg
     1741 gcgtatacat gctgtacagt catccaggcc ttataagggg tatattgatt gttttgtgat
     1801 atgtttcaat tttttgttgt tgttattgtt atatttttta aaaaatctta ctcattgtga
     1861 aacgattttt atcgtgcact gtgcttgatt acatggttac cagaggtgtc tctgtgtaag
     1921 tgatgtttct gtatgtttat tacattgaga acaaatgagt tatccggtac agaaaaaaat
     1981 aaaaacaggt gaaaatgttc tccgggctgc tgtggaacgg attaactggg tttttgatac
     2041 ttttgcggag atttgtctgt ctttttccgg aggtaaggat tccactgttt tatttcatct
     2101 ggttgcggat attgcccgca gaaaaaaacg acgtttttcc gtgttgttta tcgactggga
     2161 agcgcagtat ttatgtactg tccgccatgt acaaaaaatg cgtgaattgt atatggatgt
     2221 cacagagtct ttttactggg tggccctgcc tctgacaacg gtgaatgggg tttcccagtt
     2281 ccagcctgaa tggatatgct gggagtctgg agtggaatgg gtccgtcagc cacctgatta
     2341 tgctgttact gacccgaact ttttcccttt ttatcaatat gccatgacat ttgaagaatt
     2401 cattccggct ttttcatctt ggatggccag aagtaaacgt gggctggcag tgctgaccgg
     2461 agttcgtgca gatgagtccc tgaacaggtt tatggggctg gcttcgcagc ataagctacg
     2521 ctatgcggat gataaaccat ggacgactgc atcacaggac ggtttttact acacgatgta
     2581 ccctctgtat gactggaaaa cccgggatat atggatatac aacgcgaaaa cgcatgccat
     2641 ttataacccc ctgtatgacc tgatgttcag agctggcatt ccattacgga acatgcgtgt
     2701 ctgtgaaccc tttggccctg aacagcgtaa gggattatgg ctttaccatg ttctggagcc
     2761 tgaaacctgg gccagaatgt gtgcccgggt ctcaggagca gccagtggag cgctgtatgc
     2821 aaatgaaagt ggtgcttatt ttgcgcttcg aaaacgtata tcaaagcctg accatcatac
     2881 ctggcgcagc tatgcgatgt ttttactgaa tgtgatgccg gaaaaaacgg cggaacatta
     2941 ccggaacaaa atagccgtat atttgcactg gtaccagaca catggatatc ccgatgatat
     3001 tcctgatgag caggataatg atctggggag tcgtgatgtt ccgtcatggc ggcgtatatg
     3061 taagacgctc ataaagaatg atttctggtg ccggaccctg tccttcagcc caaacaaacc
     3121 ccgtcactat gaacgttatc tgcaaagaat gaaagagagg agaaaggaat gggggatcct
     3181 ttaacgccgg aaatactgac agtcatgatc cgtcagtatt tctgtcagca cctgtcagaa
     3241 gaagagacaa ttaatgcgct gaatcatctg cgcagggtgc ttcatgagat cagccctttt
     3301 gcccgggaac cggtggactg tgtgttatgg gtgaaatcgg atgaggtgat agccaacgat
     3361 tataatccca atgtcatggc gccgggtgag aagaaactgc tgaagcagtc actggagcag
     3421 gatgggttta cacaaccagt cgtggtatcg gaagaaaaag atcattatct ggtggtggat
     3481 ggttatcatc gtcagttgct gggaagaggg gccggtacag aaaaacgcct ccggggatgg
     3541 cttcctgtta cctgcattaa tccggagaga aaggggcagg cgacccgtat ggccgcgacc
     3601 atccgtcata accgggccag aggaaagcat cagattacat caatgtcaga tatcgttcgt
     3661 gacctgtccc gtctgggatg gtcggatgaa agaattggta ctgagctggg tatggaccag
     3721 gatgaagtgc tgaggctgaa acaaatcagc ggactgactg agctgtttca ggatgaagat
     3781 ttcagtccgg catggacggt tcgttaattc aattaaataa aattctggtg gatgagtttt
     3841 tgagtacgca gaagagagca ctggaagcag tggatgatct gatcgcactg aagcttgagg
     3901 ctgcaggatg ctggcgcagg gcttctgcac gatggctggt cgtgatgggg gcgggcgata
     3961 ttacagatgc acaacgggag tggcttttac gacgacgggc atattgcatg gcgcagacca
     4021 cttctcatgt tttacacgaa aagatgaata tccggggggt ggcaaaagct gcagatgaga
     4081 ccctgaagcg tatgggaatt gcagatctgt cagaagagat gttcagaaaa agaccatcat
     4141 actactgata acgggctctc tgtggctggc ttctgaagtg gaatatatgt gaaagagacc
     4201 ggatatattg cgctttttac tttcaataaa tcagtaaaca ggatttgtga tgtattattt
     4261 atcataatat aacaaattgt aatcagtcag agtctgcaga ctttgtcagg cttcagatat
     4321 cttattgtgg atttctgtat gtttgtagac gttttttcgg aaagacatgc tgtggacagc
     4381 aagtgttgta tgtagccctc gaattggatg caatctttgt tgctgcattt ctgatgcgcg
     4441 tttctggcat atgatttgcc ggactgtccc ttcgacctga aaagatgcca gaaacgcgac
     4501 tgttttatt
//
LOCUS       DAPHDX010000226         4385 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPHDX010000226 DAPHDX010000000
VERSION     DAPHDX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.229"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            348..1280
                     /gene="rfaD"
                     /locus_tag="RH058_004350"
     CDS             348..1280
                     /gene="rfaD"
                     /locus_tag="RH058_004350"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV3139826.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1290..2336
                     /gene="rfaF"
                     /locus_tag="RH058_004351"
     CDS             1290..2336
                     /gene="rfaF"
                     /locus_tag="RH058_004351"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV3139827.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2340..3332
                     /gene="rfaC"
                     /locus_tag="RH058_004352"
     CDS             2340..3332
                     /gene="rfaC"
                     /locus_tag="RH058_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV3139828.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3329..>4385
                     /gene="rfaL"
                     /locus_tag="RH058_004353"
     CDS             3329..>4385
                     /gene="rfaL"
                     /locus_tag="RH058_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HDV3139829.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNG"
BASE COUNT         1174 a          985 c         1061 g         1165 t
ORIGIN      
        1 ccaatgtcaa aatatgcagt gataattgtg gtggatgatt tcattactaa taagaccatt
       61 aatataaaaa aagaattata tcataaatat taacagaatg tatactgtat attatccatt
      121 caaatgcttc cgttatttat catacatcgg aatattgata ctaaagcact attcacatgc
      181 aaaaccaaca tccgccatga aggactagct aaaacccaaa ctagtttgtt gcaattagca
      241 tccttgcacc tctatgtaaa gggctgaagg gattcggatg tgatggtatg attacagaca
      301 ttcgtgtctg agattgtctc tgactccata attcgaaggt tacagttatg atcatcgtta
      361 ccggcggcgc gggctttatc ggcagcaaca tcgttaaagc cctgaatgat aaaggcatca
      421 ccgatattct ggtggtggac aacctgaaag acggcaccaa gtttgtgaac ctggtggatc
      481 tgaatatcgc agactatatg gataaggaag acttcctgat ccagattatg gctggcgaag
      541 agttcggcga tgtcgaagcg attttccacg aaggcgcgtg ctcttccacc accgagtggg
      601 acggcaagta tatgatggat aacaactatc aatactccaa ggagctgctg cactactgcc
      661 tggagcgtga aatcccgttc ctgtacgctt cttccgcagc cacctacggc ggacgcacct
      721 ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa cgtctacggt tactcaaaat
      781 tcctgtttga tgaatatgtt cgccaaatcc tgccggaagc gaactcgcag attgttggct
      841 tccgctattt caacgtttat ggaccgcgtg aaggccataa aggcagcatg gcgagcgtcg
      901 ctttccatct caacacccag cttaataacg gtgaatcacc gaagctgttc gaaggtagcg
      961 agaactttaa acgcgacttc gtctatgttg gcgatgtggc agatgtaaac ctgtggttcc
     1021 tggaaaatgg cgtttccggc atcttcaacc tcggcaccgg tcgtgcggaa tccttccagg
     1081 ctgtagctga tgctacgctg gcttatcaca agaaaggcca gatcgaatac attccgttcc
     1141 cggataagct gaaaggccgc taccaggcgt tcactcaggc agatctgaca aatctgcgcg
     1201 cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg tgtaacggaa tacatggcct
     1261 ggctgaatcg cgacgcataa gagctctgca tgaaaatact ggtgatcggc ccgtcttggg
     1321 ttggcgacat gatgatgtcg caaagtctct atcgcacgct ccaggcgcgc tatccccagg
     1381 cgataatcga tgtgatggca ccggcatggt gccgtccatt attatcgcgg atgccggaag
     1441 ttaacgaagc tatccctatg cctctcggtc acggagcgct ggaaatcggc gaacgccgca
     1501 aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc ctacgtctta cctaactcct
     1561 tcaaatccgc attagtgcct ttcttcgcgg gtattcctca tcgcaccggc tggcgcggcg
     1621 agatgcgcta cggtttactc aacgatgtac gcgtgctcga taaagaagcc tggccgctaa
     1681 tggtggaacg ctatatagcg ctggcctatg acaaaggcat tatgcgcaca gcacaagatc
     1741 tgccgcagcc attgttatgg ccgcagttgc aggtgagcga aggtgaaaaa tcatatacct
     1801 gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg tttttgcccg ggtgcggagt
     1861 ttggtccggc aaaacgctgg ccacactacc actatgcgga gctggcaaag cagctgattg
     1921 atgaaggtta tcaggtggtt ctgtttggct cggcgaaaga tcatgaagca ggcaatgaga
     1981 ttcttgccgc tttaaatacc gagcagcagg catggtgtcg gaacctggcg ggggaaacac
     2041 agcttgatca agcggttatc ctgattgcag cctgtaaagc cattgtcact aacgattctg
     2101 gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt tgccctgtat ggtccaagta
     2161 gcccggactt cacaccgccg ctatcccata aagcgcgcgt gatccgtctg attaccggct
     2221 atcacaaagt gcgtaaaggt gacgctgcgg agggttatca ccagagcttg atcgacatta
     2281 ctccccagcg cgtactggaa gaactcaacg cgctattgtt acaagaggaa gcctgacgga
     2341 tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt tctccatacg ttgcccgcac
     2401 tcactgatgc ccagcaggca atcccaggga ttaagtttga ctgggtggtg gaagaagggt
     2461 tcgcacagat tccttcctgg cacgctgccg ttgagcgagt tattcctgtg gcaatacgtc
     2521 gctggcgtaa agcctggttc tcggccccca taaaagctga acgcaaagcg tttcgtgaag
     2581 cgctacaagc agagaactat gacgcagtta tcgacgctca ggggctggta aaaagcgcgg
     2641 cactggtgac acgtctggcg catggcgtaa agcatggatt ggactggcaa accgctcgcg
     2701 aacctttagc cagcctgttt tacaattgta agcatcatat tgcaaaacag cagcacgccg
     2761 tagaacgcac ccgcgaactg tttgccaaaa gtttgggcta tagcaaaccg caaacccagg
     2821 gcgattatgc tatcgcacag cattttctga cgaacctgcc tacagatgct ggcgaatatg
     2881 ccgtatttct tcatgcgacg acccgtgatg ataaacactg gccggaagaa cactggcgag
     2941 aattgattgg tttactggct gattcaggaa tacggattaa acttccgtgg ggcgcgccgc
     3001 atgaggaaga acgggcgaaa cgactggcgg aaggatttgc ttatgttgaa gtattgccga
     3061 agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc taaatttgta gtgtcggtgg
     3121 atacggggtt aagccattta acggcggcac tggatagacc caatatcacg gtttatggac
     3181 caaccgatcc gggattaatt ggtgggtatg ggaagaatca agtagagtgt cgttctacaa
     3241 gtatgtctct tgcagatttg ccagctcaaa ccgtttttca aaatttaaat cttgaaataa
     3301 taaccaataa gttgacatcg gagataagat gacctcaaca ttatttttct ctctcgagaa
     3361 aaaaaactgg atagcgtact ggaacagagc tctcgtattc ttattcatta ccacctattt
     3421 tttgggtggg attacaaggt ataaacatct tattgttatt cttatgacaa taacgacaat
     3481 cgtctatctc tgcaaacggc caaaacacta tctctcacta tttaaaacat ttctttttgg
     3541 tagtgttgcc atattaacta ttgctgcatt gctgtcactt cttcaatccc ctgatgcagg
     3601 tgctagcatg aaggaaattt tcaaagctat tattgagaat actttactat gcacaatagc
     3661 aataccgatc atattgagag acgagaaaag agaagatgtc gaaaaaatcg ttttcttctc
     3721 atttattagt gcgttgggct tacgctgttt ttctgaattg attacctatt ataaggacta
     3781 tcaacaaggg ataatgccat tcgcagatta tagacaccgt agcatttctg actcgatggt
     3841 ctttttattc cctgcattgt taaatctctg gcttatcaaa tcagcaaaat accgcatttc
     3901 ttttgtggtt ctaagcgtta tttttatttt tctgatatta ggaactttat ccagaggggc
     3961 ctggctttcc gtgttagtca ttggattaat atggattctg atgtttaaac aatggaagtt
     4021 actattagta ggagtaatgg ttgccatcat tgcattgtcg gttattttca cacataagga
     4081 gatgaccgca aagctaacgt ataaacttca acaaactaat agttcttatc gctatgcaaa
     4141 tggtactcaa ggcagcgcac tcgatctaat attagaaaat cctgttattg gttatggtta
     4201 cggtaacgtt gcatataaag atgtctataa taaacgtgtc attgattatc cagaatggac
     4261 ctttagacaa tcaatagggc cacataattt tgcgctattc atctggtttg gcactggttt
     4321 attagggctg gtaagtctta tgatgctata ctgtgcaata ttgaaagagt gtataaaaaa
     4381 tggcg
//
LOCUS       DAPHDX010000227         4252 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPHDX010000227 DAPHDX010000000
VERSION     DAPHDX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.230"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..787)
                     /gene="wzm"
                     /locus_tag="RH058_004354"
     CDS             complement(<1..787)
                     /gene="wzm"
                     /locus_tag="RH058_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen export ABC transporter
                     permease subunit"
                     /protein_id="HDV3139830.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFEVVNKNWPP"
     gene            complement(789..2165)
                     /gene="rfbK2"
                     /locus_tag="RH058_004355"
     CDS             complement(789..2165)
                     /gene="rfbK2"
                     /locus_tag="RH058_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK2"
                     /protein_id="HDV3139831.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLSKISVLKEYVDHLM
                     SYVDFSNFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQKAFPASGEINRKLRNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     gene            complement(2189..3604)
                     /locus_tag="RH058_004356"
     CDS             complement(2189..3604)
                     /locus_tag="RH058_004356"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDV3139832.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHSAYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLIELAKYRPDIL
                     ETCQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRVEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGAYLGEDDIIRIKDQYGRC"
     gene            complement(3882..4166)
                     /locus_tag="RH058_004357"
     CDS             complement(3882..4166)
                     /locus_tag="RH058_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139833.1"
                     /translation="MSRLSHQSQHNAVVVCKELSSPNARCFSGGEYDVSSYLSSYRKC
                     PFLMVAGISKSLDSFFLMAVRACVLPRVNEKVLMVNLAANVEIVGRFVAW"
BASE COUNT         1034 a         1223 c         1063 g          932 t
ORIGIN      
        1 tcggtggcca gtttttgttg accacttcaa acatcctgaa accgatgacg cagaaaataa
       61 tggcgagagc caatactggc catagcgaat accagttggg ggccagatga taggcgaaga
      121 tggactgata agattgcatg atccgagtca tcgggttata catcatcaga ttttttgccc
      181 atgcaggtaa tgaattcagt acataaacaa tgggtgtgaa ccaaaaccag aattgcagcg
      241 caacgccaac caattgcccc acatccctga aaaagacgtt catcacacca aggatcatcc
      301 ccagcccacc ggcaaacagg atctgcaaaa gcaggaccgg tatcaccgag agaaagagcc
      361 agccggggaa gttaccggtg acaatgataa aaattagaaa caaactgaaa ataatcgcga
      421 aatttagcac cgccgataac gtcacgatga tcggcagaca gattttcgga aaactgagtt
      481 tcttgatcag attagcattg ttaataaata cgctctgacc tttatccagc atctcagtaa
      541 ataatcccca ggtcagtacc ccggaacaga gataaatact ataggcaaac gacccggtat
      601 tatcgggcat tcttgccttc atcacctcgg aaaaaaccag ggtatagacc agaatcatag
      661 agagtggttg taaaacgagc catagtgcgc ccagcatact agtttgatag cgcgcctgaa
      721 aatcacgttt aacactgctc cagataaatc cccgataacg ataaatcgtc gttagtaaat
      781 ctctcacctt attccccgct taacaggtgt aacagctcgg tcgttttttc attcataagc
      841 gccacatcag ctctggactc aacgttcaga cgtaccaccg gctcggtgtt agacgtgcgc
      901 aggttaaagc gccattcagg gtattcaata ctgatcccgt ccgttgtgtc gatgtgtgca
      961 gccgccggct catactgcgc ccggatgcgg gcgatcgcct cagcagcatt ccttagcttg
     1021 cggttgatct ctcccgacgc agggaacgcc ttctggcggt ccgccaccag cgatttcagc
     1081 gagctgttct tcaaacacag cagctccgcc accagcagcc acgggatcat cccgctatcg
     1141 cagtaggcga aatcgcggaa gtaatggtgc gcactcatct ccccgccgta gatagcgtct
     1201 tcctgacgca tccgctcctt gatgaacgca tgccccgtct tcgacatcac cggctgaccg
     1261 ccgttgcggg tcacgatgtc taccgtgttc cacgtcaagc gcgggtcgtg aatgattttc
     1321 gctcccggct gcttctgcag gaacgcctca gccagcaggc cgacaatgta atacccctcg
     1381 ataaacgaag cttcgtcatc gaacaggaag cagcgatcga agtcgccgtc aaaggcaatc
     1441 cccatgtcgg cctgatgctc gcgcaccgcg tcggcggtat cctggcggca ctccggcagc
     1501 agcggattcg ggataccgtt agggaaatga ccatccggct ggtgatgcac cttgataaag
     1561 gttaccggca ccccagccgc cgcgaagcgt ttctccacct catcaatcac gtgccccgca
     1621 gccccgtttc cggagttcac caccaacttc agtggacggg tgaagttcga gaagtccacg
     1681 tagctcatca gatggtcaac atactccttc agtaccgata tcttgctcag ggtcccgcga
     1741 cgcgccgggt ccactggcgg gaactggttt tcctccgcca ggcgctggat atcccgcagg
     1801 ccggtgtcgc cgctgatggg cttcgcattc tcgcgcacca gcttcatgcc gttgtagttc
     1861 ataggattgt ggctcgctgt cacctcgatg ccaccatcta ccccaaggtg gaaggtggca
     1921 aagtaaatct cttcggtacc gctcaggccg atgtccagca cgtcggtacc ggcgtccatt
     1981 aacccgcggg ccagcgccag cttcagcgac tcgcttgtga ggcgcacatc gccccccacc
     2041 actatcttcc cgggtttcag aaattcgccg taggcgcgac cgatacggta ggcgatgtcc
     2101 tcgttcagtt cctcacccag ttcaccacgg atgtcataag ctttaaaaca agttaactgt
     2161 gtcattctgc gtcttgtccc gaaaataatt agcaacgacc atactggtct ttaatacgaa
     2221 taatgtcgtc ctcgccaagg tacgcccccg actggatctc cagcacttcc agcggaatac
     2281 agccagggtt ttccaggcag tgctcggcgc caatcggaat aaaggtggac tggttctcgg
     2341 acaacaggaa ctgcttaccg ttgacagtca cctgaccggt gccggcgaga ataacccaat
     2401 gctcgacgcg atggtggtgc atctgcatcg agaaggcacc gcctggtttc accgtgatac
     2461 ggttgacgtt gaagcgcggg gtctggacca ccacgtcgca gcggccccag gggcggtaaa
     2521 tctcacggtg gcgcttgtac tcgctgcgct ggttctgctt gaggaactcg accgccttct
     2581 tcacgtcctg ggaacgctcg cgattcatca ccagcacagc gtccttagtg ctgacaatca
     2641 ccagattctc tacgccaatg gccgccacta gcttctcgtc gctgttgatg tagcagtttt
     2701 cgctgttgtg tacccacgcg tcgccgctaa ggacgttgcc ctgctcgtct ttcgggctga
     2761 cctcccatag tgcggaccag gagccaacgt cgctccagtc agcatcgaga ccgaccacca
     2821 ccgcatcagc ggttttctcc atgacggcgt agtccacgga ttcatccggg cactcgcaga
     2881 aaatatcatg cgggatattg atgaaatcgc ttccattatc ggcggcattc accgcagcct
     2941 ggcaggtttc caggatatcc ggacggtatt ttgccagctc gatgaggtat ttcttggcgc
     3001 ggaacataaa catgccgctg ttccagtagt actccccgga ggcgaggtaa gcctcggcgc
     3061 gctcgcggtc cggcttctcc acaaagcggg ccacctggta cgcggaatgg gcactatcgg
     3121 tgagcgccac gccgcgctga atgtaaccgt agccagtttc cggggcattc ggcacgatac
     3181 cgaaggtgac cagatgacct tcatcagcat actgctcggc cacccggata gcgtcgtgga
     3241 aggccgactg gttattgatg atgtggtcgg cggcgagaac cagcatcagc gggtcgtcac
     3301 cgtagcgggt ggcctgaagg gccgccaggg cgatggccgg cgcggtgttg cgcccgactg
     3361 gctcaagtat aatgttattc gacagcttat ttagctggcg cagctgttca gccaccagga
     3421 agcgatgctc ttcgttacag atgaccatcg gttcatggat ttcaagaccc gagagtcggg
     3481 tgatggtttc ctgcagcatg gagttctgcc cgaacaggcg gaggaactgt ttcggataaa
     3541 gctcgcgtga catcggccag agacgactgc cggtaccacc agccataatt acaggaagta
     3601 acataatgaa atccttaaat aattgtttta ttttactatc aatcaatcga ccaacaggtt
     3661 gaagggcctt tttcaggtac gctaccgcca ttggctatcg caaccaaaat gcgtagtgct
     3721 cccgcgctcc aggctaaacg ccaccctctt ttgcttcaga atgtcggcaa ccccttcact
     3781 cagcggtctc aaaaaccatc cttacggaac gggcatcctg aaaacctaaa agcagagata
     3841 tatctcacag catatctctg cttttaatat gtatctgttc ctcaccaggc cacgaaacgc
     3901 cccacaattt ccacatttgc cgccagatta accatcagga ccttctcatt tacgcggggc
     3961 aaaacacatg ctctcaccgc catcaaaaaa aacgaatcaa gtgatttact gatgccggca
     4021 accatcagaa aggggcactt acgatacgag gaaagataag aagagacatc gtattcgccg
     4081 ccagagaagc agcgcgcgtt cgggcttgac agctctttgc acacgacgac ggcgttatgt
     4141 tgcgactggt gggataaacg actcatgcct ctacactgca cattccacaa aaaaatcttc
     4201 gcagattata gcgttgcatg ctagaaaaaa aatataaaag ccgactaatt aa
//
LOCUS       DAPHDX010000228         4225 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPHDX010000228 DAPHDX010000000
VERSION     DAPHDX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.231"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(232..768)
                     /gene="yghD"
                     /locus_tag="RH058_004358"
     CDS             complement(232..768)
                     /gene="yghD"
                     /locus_tag="RH058_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV3139834.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(770..1948)
                     /gene="gspL"
                     /locus_tag="RH058_004359"
     CDS             complement(770..1948)
                     /gene="gspL"
                     /locus_tag="RH058_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV3139835.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKINQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1945..2295)
                     /locus_tag="RH058_004360"
                     /pseudo
     CDS             complement(1945..2295)
                     /locus_tag="RH058_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
     gene            complement(2270..3070)
                     /locus_tag="RH058_004361"
     CDS             complement(2270..3070)
                     /locus_tag="RH058_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001413967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial type II/III secretion system short
                     domain protein"
                     /protein_id="HDV3139836.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGQGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPPANQH"
     gene            complement(3100..3546)
                     /locus_tag="RH058_004362"
     CDS             complement(3100..3546)
                     /locus_tag="RH058_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein C"
                     /protein_id="HDV3139837.1"
                     /translation="MLHRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVS
                     APVEIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDI
                     AIALNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(3536..4225)
                     /locus_tag="RH058_004363"
                     /pseudo
     CDS             complement(3536..4225)
                     /locus_tag="RH058_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Accessory colonization factor AcfD"
BASE COUNT          930 a         1178 c         1073 g         1044 t
ORIGIN      
        1 gagggtacat atttcaccaa accaaacaaa ataaaataat atcaacatga tagaagcatt
       61 cttcgattcc gagtccggca ccactaattc ttaagaaccc gcccacaagg cgggtttttg
      121 cttttggatc tgacaataac cttcacgaaa aaagtttgct tataaagtct gggggaatta
      181 ctctcgccac gttaacgaga gtaattttat tgatactaat ctcctgatac tttacccccg
      241 tccaaactcc agccgctgca cattcaccat cccagtcttc tcagcagcac tggcatcaat
      301 ttgtgtcacc cgcagcgcat acttttcatc cagcgcgttt aaccagttca gcaggtcatt
      361 aaacaccacc ggatctatcc agacctgaat attctcccca cgctcggcta tccgcttgat
      421 gactaccgag tgcgcggaag cgctgtcact gataacccgc gatacctgcg caggcgttgt
      481 tgtggcggat tttcgcgccg ccataatatc cggcgcggcg ctcctcagtc tggtgttcat
      541 cgccaccagt tgctgcaaca ttgtctcctg ttgttctatc cgttcgctca acggctgcca
      601 gatgaggacg taatatccga cgctaaacag gaacaccacc gctgccagta acatgccctt
      661 ttcacgcgga gaacgccccg ccaggtgttg tgccagccag tgttcgccac ggcttaactg
      721 gcgttcacgc cattgctgaa aatagtgaat aaatttatcg cgtaacatgt tatttcctcc
      781 gcaacgttac gccgccggaa accgcatcac cctctttctg taatgcgtcc tgttgcacaa
      841 cataatcggt tgccagtgca ctacgcagtt tgtcgaaact ggcaaagtta gcagcccgca
      901 actggaggtg aagcgtctgg cgtttttgat caaaggtgaa accacgcatt tcgatgtcgg
      961 aaagtgacgc tgatttcagg gtgctggcta tcgctgacaa ctcggcgagc agccgggtat
     1021 cgtcggcctg tgggcgatat tttttcagcg ccatcgtcac ctgagagcgt aaattcacaa
     1081 tccgcttctg ctccgggaac aacgttaaga actgttcctc cgcctgggtg cggctttgcg
     1141 ccacctgttc gctgacgctc cataacgtca cgccccgctc cactgccagc gcaaccagaa
     1201 tcagcaatat cggcagaatc atcacccgcc agcgcgccca ctgttttcgg tagctgacgc
     1261 gaggctgcca cggccctgtc agcaggttcc cttccggttc gccataagtg gtaatggcgg
     1321 gcagagctgt aacggtcagg cgttcagcgt ctgcgccagc ccatgctgat agcttttccg
     1381 gtgcaatgcc gactacggtt agcgaaagcg gtaaattctg ctcattgagc tgggcgcgga
     1441 acatgaccgg agccagcgcc cgcccggcgc tccatccccg gcattcatcg atgcggcaga
     1501 taacccgttg tgcatcgcca gccataaacc cacaaggaat ggacatccag tccggcgcga
     1561 cggtagcgcg ggtgatgccg ttttcctgca accactgcgc aatgttgcgc atatgctgtt
     1621 ggtgaatcac ggctacggtt gccagttgct gattgatttt caacggggcg aaatgcagtt
     1681 catcgatatc ctggttcagt tcttcttcca gtaaggcggg cagaatagtc ggtatctgct
     1741 tgcgggcgac aacaggcagt tcaacctgcc agacgctgat ccattcgccg ggaatgtaga
     1801 gtcgaatcgc atcagtttgc agccattgct ggagctgttc gttagcaacg tcagaccaga
     1861 taccgtgctc cacatcgggt gtacggcgct gccaatggat gggatcagcg gcgcaaagcg
     1921 ggaaaaaaat ctcaaggatg gaactcactc actttctcct gtctgatgcc agagaaccga
     1981 aaagtgttgt gggcccatgc ggacaattaa cgagttcatc gtcagttcaa tctcattcac
     2041 ggtgatatct gaacgcagcc agaagtaatt gctgtccacg ctcaggacgg tttttagctg
     2101 ttttttagta cgctcatcga cgtcagcaag tagcggctgt gcaagaaact gatcgacatc
     2161 ttcccagccc ttcgccggac gttgttgtaa taacgcccgc gcctgaacag ggcttaacca
     2221 cgggtcaaac agcgcctcaa gaatcacact ttgcgtgacg tctaaagtat taatgttgat
     2281 ttgctggcgg gtcaccactg acaatcacac tgttggtgcg ttcgtcggcg acaatttgag
     2341 atttcagcgt cgctggctgg ttctcgccgc tgtttttcgt caggctttcc agcacgcggg
     2401 cgatttccga agccgaggcg ttatccagcg ggatcacctc ttcggtgcga ttacccgcgt
     2461 gatccacgcg ctggatcact tccgtaaggc gctccacaac ggaggcgcgt ccggtgagca
     2521 taatcacgtt ggagggatcg taattaacaa cgttgcctga gcctgcgctg tcgatcatct
     2581 ggcgcagaat cggtgccaac tcgcgtaccg acacattacg taccggcact actttggtga
     2641 ccatttcatc gcccgcgtag ttgtcgctgc cttcaccgac cagcggcagc ggctcgactt
     2701 tcgcagcgct ggatttcacc accttcagca cgtcgttttc cattggtacg acggcatacc
     2761 cctgtgcttc cagcaggtta aggaatagct ggtaatactg gcgttcattg agcggggtca
     2821 tggtgcgaat actcactttc ccctgtacgc cctgccccat aatgatggtt ttattaaggt
     2881 tagcgccgac ggtttcgatg aacgatttca ggtcggtatc tttaaaatta gcggtgaaag
     2941 tggcttcttc cgcccagacc ggtgaactgc ataatgccgc tgccagcacc agcggcagta
     3001 aacgcttttt tgttttgagg ccagttgtct tcttacgcca gaccgacaac gtaatatcac
     3061 gccaaaacac gatgaatgat tctcctggat taaatgcggt tagcgcagcg cgatggaaat
     3121 gtcgtggcgc gcacccttgc gtaaaaccgt aagttgaatg gaatccattg aaggtaactg
     3181 ccgcatcaga gcaatcattg ctcgtggatc agtgaaatcc tgctgattta gcgcaatggc
     3241 gatatcgcct tccttgaaac cgctggcatc gaacagagaa cgatctgccc ccggcttcac
     3301 tgcataaccg acaattccct ccttacgcac aggcgtaagc tggatatagt taaaaatttt
     3361 ctgcggatct ttcgccagtg cctgacgcac ggcagtcggt atctcaactg gcgcactgac
     3421 agcaggttca gcaacagctt gctttgcttc gtcactgaca gcttttttgt tggtcgcggc
     3481 aacggtggaa cgctcctctt cagccaggct caggcgctct atttttccct gataccggtg
     3541 aagtatgagt ggatggcgct ttatcggtcg cggcattgtt ttccacctct tcattgacca
     3601 gctttttgaa ctcttccggt gccagatcga tttgcttata cagcgagtcg aaacgtttac
     3661 tctcgatcac cgaggagaac tccaggcaca cctgttctgt attcgccggg caactgttca
     3721 tggaggtgac cagcgagagc gcattgcttt tcttgttgtc ggaacccgcc agttcttgcg
     3781 cgtcctcaag actaaacgac actttttcaa ccgcacgcag gctacgcgca gcttctgact
     3841 gggtgtcgaa ggtagcaatt gtcgtgttgt tccctgccac acaggtgact ttgtcgcctg
     3901 gtgtaaaggt aaagccatcg ctggattcat cattacaggt gatatcacca gttacccgca
     3961 ggcttccgcc cagagtcaga taacccgttt tcgtaggaac aggttctggc tccggttctg
     4021 gtgttggctc aggatcaggt gtcggttccg gcgtaggttc tgggtccggc gttggctcag
     4081 gcgtcggctc cgggtttggt gttggatcag gtttcacttc cggcaaagac cctgttccag
     4141 aatctacagg cggcgtatcg gaggaagatc cggagccacc gccatcacaa ccggctaaca
     4201 gggttgcact caaaatagcc gctaa
//
LOCUS       DAPHDX010000229         4178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPHDX010000229 DAPHDX010000000
VERSION     DAPHDX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.232"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2023
                     /locus_tag="RH058_004364"
     CDS             <1..2023
                     /locus_tag="RH058_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3139838.1"
                     /translation="YAYLNLQSGINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTS
                     AERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARG
                     TAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSV
                     PVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAF
                     NLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRY
                     STQGFYNLSDSAYSRMSGYTVKPPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGN
                     YGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFT
                     LNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGN
                     THGGNTSSGTSGYSSLNYRGAYGNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQP
                     LGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNVEL
                     DETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAEN
                     GQVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPEVSPETLLNQQTAICR"
     gene            2037..2567
                     /locus_tag="RH058_004365"
     CDS             2037..2567
                     /locus_tag="RH058_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3139839.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2580..3083
                     /locus_tag="RH058_004366"
     CDS             2580..3083
                     /locus_tag="RH058_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3139840.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3143..4057
                     /locus_tag="RH058_004367"
     CDS             3143..4057
                     /locus_tag="RH058_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3139841.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1193 a          894 c          958 g         1133 t
ORIGIN      
        1 ctatgcatat ttgaatttac agagtggcat caacattggt agttggcgac tgcgcgataa
       61 ctcaacgtgg agttattaca gtgggagtag caattcatct gacagcaata aatggcagca
      121 tatcaatacg tcggctgaac gtgacattat tcccttacgc tcacggttaa cggtaggtga
      181 tagttatacc gatggcgata tttttgatag tgtgaacttt cgtggcctca aaataaattc
      241 aacagaagcg atgttgcccg atagccaaca tggtttcgct ccggtgattc atggtattgc
      301 ccgtggaacc gcacaagtga gtgtaaaaca gaacggatac gatgtttatc agactactgt
      361 tccacccggc ccttttacta ttgatgatat caactctgcg gccaatggtg gtgatctgca
      421 agtaaccata aaagaggcag acggcagtat tcagacatta tatgttcctt attcgtctgt
      481 tccggttctc caacgtgctg gatatacgcg ttatgcgctt gccatggggg aatatcgtag
      541 tggaaataac ctgcaaagct ctcccaagtt catacaaggt agcttgatgc atggtctgga
      601 aggaaactgg acaccttatg gcggaatgca aattgcagaa gattatcagg ccttcaacct
      661 tggtattggg aaagatttag gactttttgg tgccttttct ttcgatatca cgcaggccaa
      721 tacgacactt gcagatggca cccgtcacag tgggcaatcg gttaaatccg tctacagcaa
      781 atccttctac cagacaggaa ccaatatcca ggtcgcagga tatcgctatt ctacgcaagg
      841 tttttataac ttatccgaca gtgcctacag tcgaatgagt ggttacaccg tcaagcctcc
      901 taccggagac accaatgagc agacacaatt tattgattat tttaatctgt tctacagtaa
      961 gcgtggtcag gaacaaataa gcatctctca gcagcttgga aattacggta cgacattttt
     1021 cagtgccagt cgccaaagtt actggaacac gtcacgcagc gaccagcaaa tatcatttgg
     1081 attaaatgtg ccgtttggtg atattacgac ttcgctgaat tacagctatt ccaataatat
     1141 atggcaaaac gatcgggatc atttactcgc ttttacgctt aatgttccct tcagtcattg
     1201 gatgcgtaca gacagtcagt cggcatttcg taattcaaac gccagttaca gtatgtcaaa
     1261 cgatttgaaa ggcggcatga ccaatctatc gggggtttat ggcactctgc tgccggataa
     1321 taacctgaat tatagcgttc aggtcggtaa cacccacgga ggtaatacat cgtctggcac
     1381 cagtggttac agttctctta attatcgtgg agcttacggt aatacgaatg tcggttacag
     1441 tcggaatggt gacagcagcc agatttatta cggaatgagt ggtgggatta ttgctcatgc
     1501 tgatggcatc acctttggac agccgctggg cgacacaatg gttctggtta aggctcctgg
     1561 cgctgataat gtcaaaatag agaaccagac cggaattcat accgactggc gtggctatgc
     1621 aatattacca tttgcgacag aatatagaga aaatcgtgtc gctcttaacg cgaattccct
     1681 tgcagataat gttgaactgg atgaaaccgt agtcactgtc atcccaactc acggtgctat
     1741 tgccagagca acatttaatg cacaaatcgg cgggaaagta ttaatgacgt tgaagtacgg
     1801 taataaaagc gttccattcg gtgcaattgt cactcacgga gagaataaaa atggcagcat
     1861 tgtcgcggaa aatggtcagg tttatctgac tggacttcca cagtcaggga aattacaggt
     1921 ttcatggggc aatgataaaa actcaaactg tattgtcgat tacaagcttc ctgaagtctc
     1981 tcctgaaacc ttgctgaacc agcagacagc aatctgtcgc taaggaacaa tagattatga
     2041 aatacaataa cgttattttc ctcggtttat gtctggggtt aaccacctat tctgctttat
     2101 ccgcagatag cgttattaaa attagcgggc gcgtcctcga ttatggctgc acagtctcat
     2161 cggattcgct taattttacc gtagatctcc aaaaaaacag tgccagacaa tttccaacga
     2221 ccggtagcac aagtccagcc gtcccttttc agattacgtt aagtgaatgc agcaaaggga
     2281 caacgggggt tcgggttgca tttaacggta ttgaggatgc agaaaataat actctgttga
     2341 aactggatga gggaagcaat acggcctccg gtttaggtat agaaatactg gacggaaata
     2401 tgcgtccggt gaaactgaat gaccttcatg ccgggatgca gtggatccca ctggtaccag
     2461 aacagaacaa tattttgcct tactccgctc gtctgaagtc aactcagaag tccgtcaatc
     2521 cgggactggt gagggcttcg gcaaccttta cccttgaatt tcaataatca gagtcagata
     2581 tgaaaagact tcacaagagg ttcctgttag ctacgttttg cgcgttattc acagcaactc
     2641 tccaggccgc cgatgtcact atcactgtta atggtcgggt agtcgctaaa ccctgcacta
     2701 ttcaaaccaa agaagctaac gttaatctcg gggatcttta tacgcgcaat ctgcaacaac
     2761 ctggttctgc atctggctgg cacaatatta ctttgtcatt aaccgattgt ccggttgaaa
     2821 caagtgcagt gacggcaatc gtgacaggtt caactgacaa tacgggttat tacaaaaatg
     2881 aaggtactgc cgaaaatatt cagatagagc tgagggatga ccaggatgcg acgttaaaaa
     2941 atggcgatag caaaacggtt attgttgatg agatcactcg taatgcacag tttccactta
     3001 aggcaagagc tatcacggtg aatggaaacg caagccaggg aacgatcgag gcgctaatca
     3061 atgtgatcta cacctggcaa taacgtcgat tcgattttct tagccttctt tttaaatcaa
     3121 ttttttaacc ttaggatata tcatgggtaa aacaatcagt ataaaggtcc tgttcggtat
     3181 ttatcttcta ttaatggcag gtaaagtttt tgccttcagt tgtaatgtcg atggcgggtc
     3241 aagtattgga gctggtacaa cctctgttta tgttaatctc gaccctgtaa tacagccggg
     3301 ccagaatctg gttgtagact tgtctcagca tataagttgc tggaatgatt acggcggctg
     3361 gtacgacact gatcatataa acctggtaca aggctcagct ttcgcaggct ctctacagtc
     3421 atataaagga tcgctgtact ggaataatgt gacctacccc ttcccgttga cgacaaatac
     3481 caacgtattg gatattggtg ataagacacc gatgccatta ccgttaaaac tctatattac
     3541 cccagtcggg gcggccggtg gagttgtcat aaaagccggg gaagttattg cccgtattca
     3601 tatgtataaa attgcaacat taggaagcgg aaatccgcgt aattttacgt ggaatattat
     3661 ttcaaataac agtgttgtta tgccaacagg cggctgcact gttgatagtc gtaatgtcac
     3721 cgtggatctc ccggactttc ctggaagcgc agaaataccc cttggcgtat attgctcaag
     3781 cgaacaaaag ctctcatttt atctttcggg tgccaccact gacagtgccc gacaagtgtt
     3841 tgctaacaca gcacctgatg caacaaaagc atcgggagtg ggtgtctctc tgatgcgtaa
     3901 tggaaaaata ctggctacgg gagaaaatgt ctccctggga acagtaaata aaagcaaagt
     3961 acctctgggt ttaagcgcaa cctatggaca aacaggaaac aaggtttcag ccggaaccgt
     4021 ccagtcagtc attggtgtca cttttatcta tgaataattg tgattttgat cttagtctat
     4081 gcgctccatt cacgaaaatt gcgcggataa ttattttgta aaggctatta tcctacacct
     4141 gtaagaaaat ccgcgcacaa ttgaagccag agtcttat
//
LOCUS       DAPHDX010000230         4150 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPHDX010000230 DAPHDX010000000
VERSION     DAPHDX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.233"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(43..678)
                     /gene="hxsA"
                     /locus_tag="RH058_004368"
     CDS             complement(43..678)
                     /gene="hxsA"
                     /locus_tag="RH058_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser repeat protein HxsA"
                     /protein_id="HDV3139842.1"
                     /translation="MKKFNFAALLPGFLALNNSVWASDSSTGASDLPGMTLNEHDLVI
                     APLNTEVPFYIAGHRSHSSHRSHSSHRSSSGGGYYGGSTPYYPKTYSSPSSSGSSTSG
                     TNSPSSSSSVRSLRSNDADASTTTNSAGMNGTTRASSGLASDTEKRKRLIMRVQFALL
                     DKGFYNGNIDGSMGPATRTAIKNYRVAYGLPTPVRETLDAQLLNSLNILAR"
     gene            complement(675..1787)
                     /gene="hxsC"
                     /locus_tag="RH058_004369"
     CDS             complement(675..1787)
                     /gene="hxsC"
                     /locus_tag="RH058_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsC"
                     /protein_id="HDV3139843.1"
                     /translation="MSEVLRNDIFRFTADLPVQQGFYRLCKSKPENPQFYLPNLLVSE
                     TQLDSRLPAYFADFVVSTDLFNSIEDGDIGIVNNGNMIRVILSRRANHNTVLVTERCN
                     NRCLFCSQPPKTGNDDWLLYQSALAIASFSLNGVVGVSGGEPLLYGEDFLQFLDFIIE
                     NSPETALHVLTNGRKFADVSFTQQMKERSEKLKITFGIPLYSSRSSVHDYLVGSEGAF
                     DETVMGLINAGNSGINIELRIIPTLANYMELDKIIEFAGRVFSNINQISLMGLESIGW
                     ARKNWSSIFIEHDSYSEKILSAIGTAQRSGITLTIFNYPLCHLPERAWGFATQSISDW
                     KNYYPKECDECTQKSSCAGYFSSSKGRFHQPPRPIL"
     gene            complement(1780..3168)
                     /gene="hxsB"
                     /locus_tag="RH058_004370"
     CDS             complement(1780..3168)
                     /gene="hxsB"
                     /locus_tag="RH058_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HDV3139844.1"
                     /translation="MKLMPFNFDRMPDGRVFISNLAGFHHFVGEQEFLDLVNGHISQE
                     ESSSLESKLFICGDESSSVTPYALSSAFAKRLMNELAIRPIFMIVPTLRCDHTCKYCQ
                     VSRASVTADGYDLNPELIPQIVSTIKKLGTPPYKIEVQGGEPLLRFDLVQSIYHECEI
                     SLGSDAFEMVIATSLSLLDESILSWVKERNITFSVSLDGNEAVHNKNRILTDFQAHNK
                     AVTGIRKITEELGANRVATVTTVTKELTKEPASIVDAHLSLGLTDMFIRPVSPYGFAQ
                     KQSFTFSMPEYFAFYKELMQEVLIQDEKGIPVIEHSAAIHLKRIFNPCFSGYADLKSP
                     SGVVLNCILFNYDGKVYGSDESRMLQKVNPEADFSAGEFASLSFSSNEYYRSALSSSF
                     NFAMPGCDTCAYQPFCGADPCQNISVHGEPVGDKSRSTFCQYHKGMFRFLLNEISQDG
                     PMAKMLKGWAYV"
     gene            complement(3168..3440)
                     /gene="hxsD"
                     /locus_tag="RH058_004371"
     CDS             complement(3168..3440)
                     /gene="hxsD"
                     /locus_tag="RH058_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HDV3139845.1"
                     /translation="MWEKILQKNLYSEWVIRNTLYWMTPVSPWKLEENTSSWIIFFEI
                     GGPECEFEFGRLLNDFSLREKLHHQTGQLRDAIISKVLRSIDDRLA"
     gene            3840..4073
                     /locus_tag="RH058_004372"
     CDS             3840..4073
                     /locus_tag="RH058_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDV3139846.1"
                     /translation="MAIPGNVWLYDDGGALIKGGCDVADREFSIEFNRFHHNLSIPTD
                     NATGKPTGTLQHSPMIIVKEFGYSSPYFYGGLN"
     gene            4031..>4150
                     /locus_tag="RH058_004373"
     CDS             4031..>4150
                     /locus_tag="RH058_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HDV3139847.1"
                     /translation="MRLFQPILLRWFKLKREDVKRTRKPLTVKIFAESAEAGKW"
BASE COUNT         1246 a          878 c          872 g         1154 t
ORIGIN      
        1 ataaatagag agttttatct tcattctatt ttacctaata ttttaacgag ccagtatatt
       61 caaagagttc aataactgag catctaatgt ttctcttact ggagtaggaa gcccatatgc
      121 aactctgtaa tttttgatag cagttcgtgt cgcaggcccc atacttccgt ctatattgcc
      181 gttataaaac cctttatcca gtaatgcgaa ctgcactcgc atgatcaaac gcttacgctt
      241 ttcagtatca gaagccagcc cactacttgc acgagtagtt ccattcattc cagcagagtt
      301 tgtggtcgta gaggcatcgg cgtcattaga gcgaagcgaa cgaacagatg aagaagacga
      361 tggcgaattt gtacctgaag tggatgagcc tgatgagctt ggtgaactgt atgttttagg
      421 ataataagga gtgcttccac catagtaccc accaccagac gaagatctat gagaactatg
      481 gcttcgatgg gagctatgac tacgatgccc tgcaatgtag aaaggtactt ccgtgttaag
      541 tggagctata actaaatcat gctcattaag agtcataccg ggcaaatcac ttgcaccagt
      601 ggaggaatca cttgcccata cagaattatt gagtgctaaa aatcccggaa gcagagcagc
      661 gaaattaaat tttttcataa aattggtctc ggtggttgat gaaaacggcc ttttgatgaa
      721 ctgaaataac cagcacagga agacttctga gtacattcat cacattcttt ggggtagtaa
      781 tttttccagt cagaaattga ctgagtagca aatccccaag ctctttcagg aagatggcac
      841 aatgggtaat tgaagattgt tagggtgatg cctgaccttt gtgctgttcc tatggcagag
      901 agaattttct cactatagct atcatgctca atgaagattg atgaccagtt tttacgtgcc
      961 cagccgatag attctaaccc cataagagaa atctggttga tattggaaaa cacgcgacca
     1021 gcgaactcta tgattttgtc cagttccata tagttagcca atgtcggaat gattcttaac
     1081 tcaatgttga tccctgagtt accggcattg ataagcccca taactgtctc atcaaatgcc
     1141 ccttcgcttc caaccagata gtcatgaact gacgaccttg aagaataaag cggaataccg
     1201 aaagttattt tgagcttttc gcttcgctct ttcatctgtt gagtaaaact gacatctgca
     1261 aactttcgtc cattggtcaa aacatgcaaa gctgtctcag gtgagttttc gattatgaag
     1321 tccagaaatt gcaagaaatc ttcgccatac agtagaggtt ctcccccact cactcctact
     1381 acaccattca gggagaaaga ggcgatggca agtgctgact ggtaaagaag ccagtcatcg
     1441 tttcctgtct taggaggctg agaacaaaaa aggcaacggt tgttgcacct ttcagttacc
     1501 agaacagtgt tatggtttgc tctccgtgac aaaatcaccc taatcatgtt gccattgtta
     1561 acaatcccta tatcaccatc ttctatggaa ttgaaaagat cagtgctcac aacgaagtct
     1621 gcaaaatatg caggaagtct ggaatcaagt tgagtttcac tgaccagtaa atttggcaga
     1681 tagaattgag gattttcggg cttagatttg cacaagcgat agaaaccttg ctgaactggc
     1741 agatctgccg taaatctgaa aatgtcgttt ctcagcacct cagacataag cccacccttt
     1801 aagcatcttt gccatcggtc catcctgaga gatttcattc agaaggaagc ggaacatgcc
     1861 tttgtgatac tggcagaaag tagaacgact cttgtcacca acaggttcgc catgaacact
     1921 aatgttctgg caaggatctg ctccacagaa aggctggtat gcacaggtgt cacaacctgg
     1981 catggcgaag ttaaatgaag atgagagagc ggaacggtag tattcgttgc tactgaatga
     2041 gagtgaggca aactctcctg cactgaaatc tgcttccggg tttactttct gaagcatacg
     2101 actttcatca ctaccgtaga ctttaccgtc ataattgaac aggatgcagt tcagcacaac
     2161 tccgcttggc gattttagat ctgcataacc actgaagcat ggattgaaaa ttcgcttgag
     2221 atgaatagca gcagagtgct caatgacagg tattcctttt tcatcctgaa tcaacacttc
     2281 ctgcatcaac tctttataaa aagcaaaata ctctggcatt gaaaaagtga agctctgctt
     2341 ctgagcaaac ccataaggac taacaggcct gataaacatg tctgtaagac ccagagacaa
     2401 atgagcatct actatggaag ctggctcttt agtcagctcc ttagtaactg tggtaaccgt
     2461 agctacccgg tttgcgccaa gttcttctgt gattttccga ataccagtta ctgctttgtt
     2521 atgagcttga aagtcagtaa ggatgcgatt cttgttatgg acagcttcat tcccatcgag
     2581 cgagaccgag aaggtgatat tccgctcttt aacccatgag agaatgcttt catcgagtaa
     2641 tgacagactt gtagcaatca ccatttcaaa agcatcacta cccaatgaaa tctcacactc
     2701 gtgatagatt gactgaacaa gatcgaacct gagaagtggt tcaccgccct gtacttctat
     2761 cttgtaggga ggtgtaccta gtttctttat ggtgctgaca atttgcggta tcagttcagg
     2821 attgaggtca taaccatcag cggtgaccga tgccctactg acctggcaat atttgcaagt
     2881 atgatcacaa cgaagagtag gtacaatcat gaagattggc ctgatcgcca gttcattcat
     2941 gagtcgtttg gcaaaagccg aacttagtgc ataaggggta acggaggaag attcatctcc
     3001 gcaaatgaaa agtttgcttt caagcgatga ggactcttct tgactgatat gaccgtttac
     3061 cagatcgaga aattcctgct cacctacaaa atgatgaaag ccagcgaggt tactgatgaa
     3121 tacccggccg tcaggcatcc tgtcgaagtt aaaaggcatg agtttcatta tgcaagcctg
     3181 tcatctatgc ttcggagaac cttgctgata atggcatcac gcaactgacc agtttgatga
     3241 tgaagtttct cgcggaggct aaaatcgttt aacagccttc cgaattcaaa ctcacattca
     3301 ggaccaccta tctcaaagaa gataatccac gaggatgtgt tttcttcaag tttccatgga
     3361 cttacaggag tcatccagta tagggtgttt cggataaccc attctgaata aagatttttt
     3421 tgtaatatct tttcccacat cgccattacc ttccaaacga aataataacg gaaagaaact
     3481 tattccattt agggattttt cttatagatt aatgatataa cacttttcaa aacatgttac
     3541 tgagtatgtc aaaattctga gattcagttc aactagaaat taatcgaatc acgcactcac
     3601 atgatgcaga agtaccattt ttcaatggtt aacctgttga agtaaaggaa caaagagatt
     3661 actagctatg ttctagttta ctggtcgcgt actattctgt gaatgtcggt gtgattggta
     3721 gaagcagata acttactctt ccagggagta aaaaaaaacc acacattggt tatttactca
     3781 cctccactca atagatacac tatttaaagg gatttgcaac gacataagga aaaaagaaca
     3841 tggctattcc gggtaatgtg tggctctatg acgatggtgg agcactaatc aaaggaggtt
     3901 gtgatgttgc agacagggaa ttcagtatag agtttaatag gtttcaccat aatctctcca
     3961 ttcctactga taatgccact ggcaagccta ccggcactct tcagcactca ccaatgatca
     4021 tcgttaaaga attcggctat tccagcccat acttctacgg tggtttaaat taaaacggga
     4081 ggatgtaaaa cgaacccgta aacctttaac tgtcaaaata tttgcagaat cagcagaagc
     4141 tggaaaatgg
//
LOCUS       DAPHDX010000231         4147 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPHDX010000231 DAPHDX010000000
VERSION     DAPHDX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.234"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            227..835
                     /locus_tag="RH058_004374"
     CDS             227..835
                     /locus_tag="RH058_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV3139848.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="RH058_004375"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="RH058_004375"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV3139849.1"
                     /translation="MTTEMRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4147
                     /gene="selB"
                     /locus_tag="RH058_004376"
     CDS             2321..>4147
                     /gene="selB"
                     /locus_tag="RH058_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV3139850.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT          932 a         1049 c         1221 g          945 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgttggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgatcgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaatgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgctgga cgcggacatc
     1321 gcgatcgtgc gctggcgcag ttgctgtgcc gcattacggg ggcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc cccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttaatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccaggacg
     2821 tgaacacgcc agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc
     3001 aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgctgc gcgatatctc
     3301 ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcatta
//
LOCUS       DAPHDX010000232         4050 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPHDX010000232 DAPHDX010000000
VERSION     DAPHDX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.235"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..1711
                     /gene="dpiB"
                     /locus_tag="RH058_004377"
     CDS             53..1711
                     /gene="dpiB"
                     /locus_tag="RH058_004377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV3139851.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1680..2360
                     /gene="dpiA"
                     /locus_tag="RH058_004378"
                     /gene_synonym="citB"
     CDS             1680..2360
                     /gene="dpiA"
                     /locus_tag="RH058_004378"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV3139852.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="RH058_004379"
     CDS             complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="RH058_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV3139853.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1109 a          951 c         1045 g          945 t
ORIGIN      
        1 acattttaat tacatccgtc aaagaggctc gggacaaccc gtaaggaaaa caatgttgca
       61 gcttaacgag aataaacagt ttgcattttt ccaaagactg gcatttccgc tgcgtatctt
      121 tttgctgatt ctggtgttct caatatttgt cattgcagcc ctggcgcaat attttacggc
      181 cagttttgag gactatttaa cgcttcatgt acgcgacatg gcaatgaatc aggcgaaaat
      241 tattgcctcc aatgacagtg tcatctctgc ggtgaaaacg cgtgactaca aacggctggc
      301 gaccatcgct aacaaattac aaagagatac cgattttgat tatgtggtga ttggggaccg
      361 gcactcgatc cgcctttacc atcctaatcc ggagaaaatt ggttatccta tgcagttcac
      421 caaacagggc gcgctggaga aaggggagag ctacttcatt accgggaaag ggtcaatggg
      481 gatggcgatg cgcgccaaaa cgccaatctt tgatgacgat ggaaaagtca tcggcgtggt
      541 gtcgattggc tacctggtga gtaaaatcga tagctggcgg gctgagtttt tattaccgat
      601 ggcaggcgtg tttgtcgtgc tgttagggat tctgatgttg ctgtcgtggt tcctggcagc
      661 gcatatccgt cggcaaatga tgggcatgga gccaaagcaa atcgcacgcg tggtccgtca
      721 gcaagaggcg ctgtttagtt cggtttatga agggctgatt gcggtggatc cgcatggtta
      781 cattaccgcc atcaatcgta acgcaagaaa gatgctgggg ctgagctctc ccggacggca
      841 atggttgggt aaacccattg ctgaagtggt caggcccgcc gatttcttta ccgaacagat
      901 tgatgaaaaa cgtcaggatg tggtggctaa ctttaacggt ctgagcgtta ttgccaatcg
      961 ggaagctatt cgttcaggtg atgatttgct gggggccatt atcagctttc gtagtaaaga
     1021 cgaaatttcc accctcaatg cgcaactgac gcaaataaaa caatacgtcg agagccttcg
     1081 tacattgcga cacgagcatc tcaattggat gtcgacgctc aatggtctgt tgcagatgaa
     1141 agagtatgat cgcgtgctgg cgatggtgca gggggagtct caggcccagc aacagcttat
     1201 tgacagcctg cgtgaggcgt ttgccgatcg ccaggtggcg gggctgcttt ttggtaaagt
     1261 gcagcgcgcc cgggaactgg ggctaaaaat gatcattgtc cccggtagcc agctttcgca
     1321 actgccgcca ggactggata gcaccgagtt tgcagccatt gtgggcaatt tacttgataa
     1381 cgccttcgaa gccagcctgc gtagcgatga aggaaacaag atcgttgaat tattcctcag
     1441 cgatgaaggc gatgatgtgg tgattgaagt cgccgatcag ggctgcggcg ttccagagtc
     1501 tctacgagac aaaatatttg agcagggggt cagtacgcgt gctgacgagc ccggtgaaca
     1561 tggcattggg ttgtacttga ttgccagcta cgtaacgcgc tgcggtggtg ttatcactct
     1621 cgaagataat gatccctgcg gtaccttgtt ttcaatctat attccgaaag tgaaacctaa
     1681 tgacagctcc attaacccta ttgatcgttg aggacgaaac gccgctggca gagatgcatg
     1741 cggaatatat tcgtcacatt cccggattca gtcagatatt actggcggga aacctggcgc
     1801 aggcccgaat gatgatcgag cgttttaagc cggggctaat cttgctcgat aactatcttc
     1861 ctgacggcag agggattaat ttattgcatg aactggtgca ggcgcattat cccggcgacg
     1921 tggtgtttac cactgcagcc agcgatatgg aaacggtgtc tgaagccgta cgttgtggtg
     1981 tatttgatta tctcattaag cccattgcct atgaacggct ggggcaaacg ctaacccgtt
     2041 tccgccagcg taaacatatg ctggaaagta ttgatagcgc cagccagaag caaattgatg
     2101 agatgtttaa tgcctatgct cgcggtgaac ctaaagacga actgccgacc ggcattgatc
     2161 ccttgacgct aaacgcggtg cgaaaactgt ttaaagagcc tggtgtgcaa catacggcag
     2221 aaacggtggc gcaggcactg accatcagtc gcaccactgc caggcgttat cttgaatatt
     2281 gcgccagccg ccatctgatt attgctgaaa ttgttcacgg caaagttggc agaccgcaac
     2341 gcatatacca cagtgggtga catgataaag ccggaggaaa cttgcctccg gcattactat
     2401 ttacttgcct gtgaccgctg ctgccgttcc tggcaccatc agctctgtag caacgataac
     2461 aatcaccaaa ccaacaagca ccggtaccga ggtgcgtttt acgacttcaa acggcgagat
     2521 cttcgccatc ccagcaaccg caacgactac gccagaaacg ggcgaaaggg tacggcccag
     2581 gtttgacgcc tgcagcatcg ggatagtcaa atacgccggg ttaatgccgg aagagtgcgc
     2641 cagtttcggg atcatctcaa caaacgcata aaacggcgca ttgcctgaac cggtcgtgac
     2701 tgccgccagc atggtcagaa tcaccaatac cagcatcagg atgatactcg ccgaaccaaa
     2761 cgaggtagcg atagaaatca gactttgaat aaagccgatg gtgctaagcc cctgagcgaa
     2821 taccccagcg gcaaccagca gcatcaccac gttagcaaac gcatctgcca ttccgcgata
     2881 agccacttcc agaccagaga aaactttctg ggtattaaag ctgcggagga actccagaat
     2941 ggaggcaatc agcatacaaa tcaccagaat agtgatgatg tgtaattgcg gaccccattt
     3001 accgtcaaaa atcagtacac cgatgatcgg cgtgaacggc aaaatggcat aaaacgcagg
     3061 agcagtggtg gtgatttcac tgacatctaa catttcatga gagatgtgct cttttttatc
     3121 cagataacgt tgccagaaga agtgggcgat cgccatgccg ataattgcag caattgagat
     3181 aggcagcgtc gttttgaagg cgaagtcaat cagcgacatt tcggaagctt gcgccgccag
     3241 caccacatcc cctgaagtcg gtgcgagaat aatcgccgcc ggggaggcac aaatggcagc
     3301 agctgcgcca cgactgatac caacgtttac catcaccgga aatagggttg ccatcagcaa
     3361 aacacccaga ccggttgcgg aagagacggc cagagacatc agacaggcga caaaataggc
     3421 ggcaatcatc agcaggtaag gggagttaat atactgcaat ggttttgacg ccagcttgac
     3481 caccatatca ttcgcgccga tatgggtcat gtaagcggca aatccacaca gcatcataat
     3541 catcatgccg aggtcgccgc cgcggctcat tagtaatatt ttaacgtatt caacgatatc
     3601 cgtggcgctg tagcctgttg aagcctggct ggacggtaac actttgtgcc ccataatggc
     3661 actgataatc agcaataaca ggccaccgac aaataacaca ccagtggcgg aatacccttt
     3721 aatgatgtag cgagctacac ccacaataac cacaacccca ataaggagtt caatgaatgt
     3781 cagcataatt tttcctgtct ccaggcccca aagtaaataa taaaaaattc ctaaagctta
     3841 aggaaaaaat atgcccaata aattggcgat gaatgctgat taaaatcaag aaaaactgcc
     3901 attaagacat tgaagttgct gtttttatac atagataaca aaacgcatat ttagcggttg
     3961 gcaattatca cataataatg tgtgtaaatt gatggtggat tgatgcaaat ttgttaattg
     4021 caatggtgtt agctgcggct gtgcgctcaa
//
LOCUS       DAPHDX010000233         4047 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPHDX010000233 DAPHDX010000000
VERSION     DAPHDX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.236"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(158..3940)
                     /gene="rhsD"
                     /locus_tag="RH058_004380"
                     /pseudo
     CDS             complement(158..3940)
                     /gene="rhsD"
                     /locus_tag="RH058_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
BASE COUNT          658 a         1367 c         1133 g          889 t
ORIGIN      
        1 ccatatcatt acatagagat ctattctgcg tttctttcaa agcatctaac gcagcatctg
       61 cagtactatc aaatttactt ggtcctatta tacgtgaaag aacaccacaa actccccccg
      121 tacatgctcc agatctggca tcatcccaag tctgcaataa tcccataggg tcaatttgtt
      181 gtagtggatt taaaggatac tgatataaat tccatccccc tttcaacccc atcgggtcct
      241 gagtaatata ccgcccctgc aacggatcgt agtaccggtg acggttatag tacagccctg
      301 attcctcatc atgctgctgc cctggcagac ggtacggctg atacacatga tgcgggttct
      361 cctcattaag ctggttgccc cattcatcat attccgcgct ccacgccgta ttgccgtctt
      421 cgctgataag cgccagcggc agtccccggt ggtcgcagtg ataaagatga gcttttcgcg
      481 ccggtgtgta ttccggctcc acctgtctgg ccagttgctc caccgtcagc ccgcactgcg
      541 caagccacgc ccggctttca ctgctcacgc ggtctgcccg gatttcttcc tccagtctgt
      601 ccagcagccg caccagttca gccggaaata ccacgccgtg cccgtcctcg ctcccttcct
      661 gctgcagctt ctccgccagg ctgcggcgct gcgctttctc ccgctcgccg ttgtctgttt
      721 cgatgcggat gagcggcgcg aagctccccg gctgatatac cgtctggata cgcgtggtgt
      781 cggtctgtac cgtcgtcagc cggtcgccgt cccacccgta ccacgtcacc tccggtttcc
      841 gcgacagcga catccatccc gtcaggtcac gctcgcgacg ccacacccgt ttccctgtcc
      901 ggcgacccag cgggtcgtaa aggtatcggc tctcgaccag cggctcctca tattggatac
      961 ggatgtaatg gaccaggcgg tgcaggctgt cgtaatggta gtgatgggtg cgctcgtcgt
     1021 ccgtccgtat cacacccgcc gggatgcggt ccgtcttctc cgtcagtctg ccgtattcat
     1081 cgtagtggta aacatagtgc gcatccttcg cgatgcggtt atccggccac actgtgaggg
     1141 tactgtccgg gtgcagctcc gggtccggca gccggttgcc cgccgggtcc gtggcatacg
     1201 ggatgcggat gtccaggtcc ggtgcgaggg tgcgcacgct ctccagcctg cccgtggcac
     1261 tgtagccgta ttcccgcgtc tgtcgcgggc cgctgatgcg caccaggtcg ccgttgtcat
     1321 tccacccgta gtcacggtca tacaccaggc tgttcaggtg ctggctctgt aactggcctg
     1381 cgggggtgta tgtgctggtc agttcatatg cggcattact gcctgccctg ctgccgaagc
     1441 tgcgcaccgt ctcccggtgc agcctgtccc gcgtatactc gaccagcggc gtcccgccca
     1501 gcttcatgcc cgccaggtaa ccgctgccgt aggtcagcca ctccaccggc ggcaggctgt
     1561 ccggcgtgac gcggtttgcc agcccctgtt cgttgtatgc gtgtttcgtc tcatgctgcc
     1621 acagcagttc ccccgtctcc gggttctcca ccgtctgccg ttcgcccgtc aggcggccct
     1681 tatcgtcgta gccgtagtgg acagcgacac ggtggccttc gctcaggtgg ctgatgtctc
     1741 tcagccagcc gtggccgtca tactgccact gctctgccgg gtcgccgttc accgtccggt
     1801 gcgtgatgcg gtccgacgca tcgtagtgcc agagggtgac aagcccctca tcttcactct
     1861 gcgtgagttt tccggtcagg tcataatgat aacgttgcgt ccgcccgtca aagccgccct
     1921 gctgtaccag ccggtccagc gcatcgtaac tgaagacgct gtggctgccg ttctcgttgg
     1981 tcaggctgat gacacgtccg gcagcatcgt actccatact gcgcgtcagc ccgccctgcg
     2041 tggtgctgac cgcctttccc cacgcatcgt actgtgtctc gctccggttg ccgtccgggg
     2101 tgataacggc agtcaggtcg cctgcggcgt tgtattcata ccgcgtttca cggccctgtg
     2161 cgtctttcac cgaggttaac cggccacggt tgtcatagcg gcggtaaagg ctgatgcctt
     2221 cctcgcggtg gaccgccgtc atctggccga agcggtcgta ttcataacgg gtctggtagc
     2281 ccgagcagtc ggtgaacgcc agcaactgcc cgtagcggct ccaggtcatc tgccgggtgc
     2341 tgcccgtcgc atctgttgtc gtcgccggta actcactgtg cgcgtcatcg tagcggtagc
     2401 gtactgtctc cccgctgcgc gatgtctccg ataccagcct gcccggttca tcatattccc
     2461 ggcggctctc cagcccgtcc ggggacacca ccgccgtcag ctggttcccg tcgttatagt
     2521 aaaatttcgt ctcccgcccg tccggtgtgg tgatgtccgt gatatcgccg gacaccacat
     2581 tcagaccgta ctctgtccgc cgtcccgccg cgtccgtctg cgccgtgagc cttcctgccg
     2641 cgtcataccc gctgcgcgtg acgctgccgt ccgccagttc ttttttcacc acccgtttca
     2701 gcccggcccc gccctctgta tgcagcacct cacgccggtt caggctgtcg gtgacggtga
     2761 tgcggtcctg ctcataaaga tagcggtagc ttaaccctgc cgggttcagt tgctccacca
     2821 cccgccccgt atcgtcgtag cggtagcgca tctccggcct tcccgcgtaa cggtgcgcca
     2881 ccatccggcc cgggtgctgc gcgtcatacg tgaaagcgcg cacctgcgta ttgctgcggt
     2941 catataccgc cagcagttca ccggcttccg tatacgtgta ccgcgccagt ggcgcaccgg
     3001 gcaggctttc cgggtatgcc gggtcgtgca tcagccacac cgccgaaaga cggatgcccc
     3061 tgtcggggcc gtattcggta ccgggcagtg tgtcggggaa cgctgaggct gagagagggc
     3121 ggggtgtgtc aggggaagat aaggaagcgg tgcgctgttt acgggcctct tccgcacgct
     3181 gcgcctgcgt ggtcagcacc agacggaact cccgcccggc accgtccgtc acgccggtga
     3241 tttccccggc caggtcaccg gcggcctcac gccggtacgt cagcgtccgc ccgaagcggt
     3301 ctgccagccc ggtaagcacc cggtacggcg gcagcggcgc tggcagtacg tcctcagcac
     3361 ccggcaccag ctctgaccac cccagtatcc accacggccc ctgtgcgctg ttggtcgcca
     3421 ggtaaagatg cgggcttaac cggatatccg gcggcagcgc cccccacagc cgcgccagcg
     3481 tatggccgtc cggctgtgct gccttaccac cgcgcaccag ccacattgac tcactgcggc
     3541 tgtacaccgc ctcccccggc agcagcggct caaagtgaat gctccgcccg ccgttgtcgt
     3601 tgagtatcag tccgtcatca cgtagctgta agcggatatc agaaggcgct ttccagccgg
     3661 ggccgaaaac gcccaccggt gcaggcgtct tcgtccggta gctgctgtag gtgcgggaga
     3721 gaatgaacgg cagcgggccg ggcagcgcaa ggtccgtctc gccgggcagc accttcgccc
     3781 ccagcagcgg atttaccggg ttgcccgaag tcatcccgcc cggacacacc gagcacgcca
     3841 cgccggtggg cgcgccaatt cttacacctg ccgaaccctg gacaatggga ccgccatact
     3901 gagtcatatc tccctgacgc gccgctggtt ttccgctcat ataaagtgcc cttttagtta
     3961 ttgttcatat ccctgtgtta gcaacaaaaa aaataaaatg acacacaaga ccagctaatg
     4021 tatttatata tttttcacat aaactta
//
LOCUS       DAPHDX010000234         4046 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPHDX010000234 DAPHDX010000000
VERSION     DAPHDX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.237"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..902)
                     /locus_tag="RH058_004381"
     CDS             complement(<1..902)
                     /locus_tag="RH058_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3139854.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YEMQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPHR
                     AKEARPFRLAVIQLGTYDGTIYNAR"
     gene            complement(1667..2004)
                     /locus_tag="RH058_004382"
                     /pseudo
     CDS             complement(1667..2004)
                     /locus_tag="RH058_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604131.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
     gene            complement(1982..2341)
                     /locus_tag="RH058_004383"
     CDS             complement(1982..2341)
                     /locus_tag="RH058_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HDV3139855.1"
                     /translation="MTLLLNDKQYNELCEAAEGRNLGAVFSYSEPEEPPPLNFSFEER
                     KKVFLWVLTRLLKEGRIKLAKHGKFLEGSVEEQVERFRQAFPKTEEEMEDGIWFFDES
                     CPGGAVWGLEDGSLEWT"
     gene            2490..4025
                     /locus_tag="RH058_004384"
                     /pseudo
     CDS             2490..4025
                     /locus_tag="RH058_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1151 a          915 c          901 g         1079 t
ORIGIN      
        1 cgggcgttat aaatcgtacc gtcgtaggtg cctaactgaa tcacagcgag gcggaacgga
       61 cgcgcctctt ttgcccggtg cggtgcaact tctgtaatca gttcacgcag ataactttct
      121 tcgaagcagt gcgcatcaat accaccgata aaaccatacg ggttacgtgc ggtttccaga
      181 taaaccggtg ttgcaccagc ctgtagcaac gctccgtggt ggttagattt gtggttattg
      241 cggtcaaaca gcaccagatc acccggtgtc agcagggcgt ttaaaaccac tttgttggag
      301 gaggaggtgc cgtttaaaac aaagtacgtt ttatcggcat taaacacttt agccgcatgt
      361 tgctgtgcaa tgcatggcgc accttcatga atcagtagat cgcccattgc tacgtcggca
      421 ttacacaagt cggagcggaa gagcatttcg ccaaaatatt ccacgaactg attgcctgcg
      481 ggatggcgac ggaaaaactc accgccctga tgacccgggc agtcaaaagc gctattgccc
      541 tgattgacat aatcgaccag tgcgcggaag aagggcgggc gcagttgcat ttcataatgg
      601 ctggcagcgg tttctaactg acgtccgtaa aactctttcc gtgattcgca atgctcgaag
      661 acaccagaaa tacgtggcag atattctgcg ggtacacgtt cttcattttc cgttgcaata
      721 aaaacaggaa tgccgtagcc agtagcgtct atttcatcga gttttccacg ttcaacatca
      781 ttgactgata aaataatggc ggcaacgtca atataattac tttcgtcaat attcacgcat
      841 gcacgatgag tggaaaaaca atccgggcaa gaacgactaa cggcaatttt taattctgac
      901 attttgttca tctctttatt tcaggtaata gcaggtcctc aatttctcaa aggagaaatc
      961 gatcgcgccg gaaaaaagca aaactccaca gctgataaat aaatcagtag tttgcttttt
     1021 gcagatgcaa taacccagac cagcccggta actggcgata ctcatcatac ttcaagttgc
     1081 ctatgctgcg actcgaatta tgtagagttt aaaagggtta gcaatatatg agcaccgagg
     1141 gatacgggta ttaacctgta atgcttaaag tcagtgagac ctgtacgctg gggtgccctt
     1201 tagtcagaag actaacgggc attaaagaaa tgaaagtcaa aactattacg atgggcaaac
     1261 ttcatgatat gtgttacgcg cctgatatgg ggcatttttc ggttattgtt ttccatttca
     1321 tatacctttc tgtatcacta gaaaacaata atattttatg tgtagagtag tcactttctg
     1381 tgaagaaggt caagaaacat tgatttcatc tgaattatct aaacaaaaat tttcgccaac
     1441 ttaaagttgt aaggcatgtt ttaaaaatat gttaaccaat tggagcgtta ttctatcctc
     1501 cccgcggcaa ttactctgtc ctttttgtaa tccgagtatt atatgtataa tttcatacaa
     1561 agtatgggtg tgtattatcg tgaaggagat cggtggggat aacctccatt gaaattggag
     1621 ttactcgccg atcactttaa caataatacg catctgtggt atcattcccc accacctcgc
     1681 cggaggcaac acctgcagca ccggtaaggg tattatttcc ctgtgccacc gccagtacag
     1741 cattcaccac ggcatgggct accacacgcc ccgcaccgtc cgggggtctg tttgataatt
     1801 tcagcaatat acggcgcagc accatctgcc aaggctgcac tcagattacc gccagccagt
     1861 ccctgtaccg ctgccgttgc agcctgtatc gcctgctggt attgcccccc atcccaaaac
     1921 cggagccgcc atattgttgt tatacgctcg ctgcgccgca tcctgcgctg tgaagggttt
     1981 atcaggtcca ttcgagagag ccatcttcaa gcccccaaac agctcccccc ggacaactct
     2041 catcaaaaaa ccagatgccg tcttccatct cttcttctgt tttcggaaat gcctgtcgga
     2101 aacgctcaac ctgctcttca acacttcctt ccagaaactt accgtgcttg gcgagtttga
     2161 tacgcccttc tttcagaaga cgggtcaata cccataaaaa aacttttttc ctttcttcaa
     2221 atgaaaaatt taacggcggg ggttcttctg gttcactata ggaaaaaaca gcccccaaat
     2281 tacgaccttc tgcagcttca cataattcat tatattgctt gtcattaagt aataatgtca
     2341 tttaaatttt atctccacat ctttgtattt atatgcaatg tcagaaagtt gtttattccc
     2401 cttaatgcgt attttcatga aaggagatca ctcaacaacc ccttgtgtca gcatagttgt
     2461 cgcctagatt tttacacagg agagagacta tgcccgcaca tgcagtacca attgatataa
     2521 aagaacaggg cgttacctgg ttattaccac cttatagttg gtccatacgg catattgctc
     2581 gtaaactgga cctgagccct tcagtaatag caagatggag acttgaactg gtgcaacaag
     2641 gcctgttacc tgaaaatgaa aaaatatctt ctgtcgatgg aaatgaatgg acaccggagc
     2701 gacgattttc tgttgtaatt gaaactgcaa cattatctga aatggaactg gctgattatt
     2761 gtcggagaat ggggttattt gtcgagcaag ttaaagaatg gagagctata tccattcagg
     2821 ctcatgaaat gaaaatggca gaaagtcgcc gtgctgataa agaattacgc aaagcaaaga
     2881 cccggttacg ggaactggaa aaagaacttc tccgtaaaga taaagctctg gcggaagcaa
     2941 ctgctctttt gatgttaagg gaaaagttca atgccctctg ggacaacagc gaggaaggct
     3001 gactcctctt ccagagcggc agaagattat taatctggtc agcgaggctg tcagttcagg
     3061 agcgagaaaa acacaagcct gccagtgtat tggtatttca gtcagaacac ttccacgttg
     3121 gatgcagggt ggtgtggttc atgcagatca gagaccaggt gcatcaaggc gtaccccggg
     3181 gaataaactg tcagaaacag aaagattaag aatacttgat gtatgcaatt ctccggagtt
     3241 tgcatcattg tctccgaata ttattgttcc gactcttgcc gacagagggg tttatatcgc
     3301 ttcagaatca actttttatc gcgtattaaa atcagctaac caactgacaa ggagaacccg
     3361 tgaacaccaa tataagcggc ctgaagtact caaagctgta gcccccaacc aggtgtggat
     3421 ctgggatatt agttatatcc catcatgggt taaggggcag catttttatc tctatatgat
     3481 cgtcgatatt tatagccgaa aaattatagc tgccgaagta tttacgaaag aatctgggca
     3541 gtatgcggca gaactgcttc aacgtgctgt atggaatgaa aaatgttcat cccgcaatct
     3601 cgtgcttcat tctgataacg gaggaccaat gcgcagttgt acgttactgg caaaaatgta
     3661 cgctcttggc gtattaagtt cttattcccg tcccctggtc agcaatgata acccttattc
     3721 cgaatcgctc ttccggacga ttaagtattg tccatggtgg ccagagaatg gttttcgaac
     3781 catagatgaa gccagaagtt ggttaagcag gttcacgcga tggtacaacc tggaacacaa
     3841 gccagcggta ttaagtatgt tactccggat gaacgccatc gcggaattga tgcacaaata
     3901 ctggaagcca gaaaaaagtt tatcgcgaag ctcgcaaacg tcatcctgag cgctggagca
     3961 aacagttgcg tgactgggaa ctcattcagg cagtttatct gaatccagaa aaagaagcgg
     4021 cataactgta attttattac atcagg
//
LOCUS       DAPHDX010000235         3915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPHDX010000235 DAPHDX010000000
VERSION     DAPHDX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.238"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            38..589
                     /locus_tag="RH058_004385"
     CDS             38..589
                     /locus_tag="RH058_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDV3139856.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            649..3153
                     /locus_tag="RH058_004386"
     CDS             649..3153
                     /locus_tag="RH058_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3139857.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3169..>3915
                     /locus_tag="RH058_004387"
     CDS             3169..>3915
                     /locus_tag="RH058_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3139858.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRNFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
BASE COUNT         1222 a          820 c          795 g         1078 t
ORIGIN      
        1 cattcatttt ctacttatat ttatataaag gaatattatg tctgctttta aaaagtcact
       61 tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg aaggtcacgg
      121 cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac caggtgacga
      181 agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg accagaaatc
      241 tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg gtggaaccac
      301 ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc agactgacct
      361 gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg tacgtattct
      421 ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat tcaacaatac
      481 taacagctat caggaactga acttcaaagc tcgtatggaa tccccaagca aagatgctac
      541 cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat cacattaggc
      601 cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat gtataaaaaa
      661 ttaaaattaa ccacaataag cgaattgatt aaaaatattt attgttcatt atccgttctc
      721 atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca aagatttaat cgaagccgaa
      781 gatcgtgaaa acgttaacct ttcccaattt gaaactgatg gccaattacc cgtaggtaaa
      841 tactcattaa acgctcttat taataataaa aggacgccaa tccatctgga cctgcaatgg
      901 gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac attattaggc
      961 tttactgatg agattattga agtggctcag caaaacctga tcgatggttg ttaccctatc
     1021 gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt atccatatct
     1081 gcacctcagg catggttaaa atacaaagat gcaaactgga cgcctcctga actttgggat
     1141 catggtattg ctggggcatt tcttgactac aatttatatg cctctcatta tgcaccacat
     1201 cagggcgata attcacaaaa tataagttcc tatgggcagg ctggggttaa tcttggggcc
     1261 tggcgcctgc gtactgatta ccagtacgat cagtcattta acaatggcaa aagccaggcg
     1321 aacaacctgg attttccgcg tatttatttg tttcgcccaa tcccagcaat taatgcaaaa
     1381 ctaactatag gtcaatacga tactgaatcc tctattttcg actctttcca tttttctggc
     1441 gtttcgttga aaagcgatga gaatatgttg ccgcctgatc tacgtggtta cgcaccgcaa
     1501 atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct cacagaacaa ccgtattatt
     1561 tatcaagaaa atgttcctcc tggcccattt gctattacta atttattcaa tacattacag
     1621 gggcaactgg acgtcaaggt tgaagaagag gacgggcaag tcacgcaatg gcaagttgca
     1681 tctaatagta ttccttatct gacgcgtaaa gggcagattc gctacaccac tgctatgggt
     1741 aaaccgacca gcgttggtgg tgattcctta caacaaccct tcttctggac tggtgaattc
     1801 tcatggggtt ggctgaacaa tgtatccctg tatggtggtt cagttttaac aaaccgtgat
     1861 tatcaatctc tggctgccgg cgttggcttt aaccttaact cattgggttc attatctttt
     1921 gatgtcacac gatctgatgc tcagttgcat aatcaggata aagaaacggg ttatagctac
     1981 cgcgctaact attcaaaacg ttttgaatct accggtagcc agctcacttt cgctggttac
     2041 cgtttctctg ataaaaactt tgtgacaatg aatgaatata tcaatgacac taaccattac
     2101 acgaattatc agaatgaaaa agagagttat attgtcacgt ttaaccagta tcttgaatca
     2161 ttaaggttaa atacatacgt aagtttggct cgtaatactt actgggacgc cagcagtaat
     2221 gtgaattatt cattatcact tagccgcgat tttgatatcg ggccattaaa aaacgtctcc
     2281 acttctctaa catttagccg aataaactgg gaagaagaca accaagatca actgtaccta
     2341 aatatttcga ttccctgggg aaccagtaga acattgagct atggtatgca acgaaatcag
     2401 gataacaaga tttcgcatac tgcttcgtgg tatgactctt ccgatcgaaa taattcctgg
     2461 agcgtttctg cttcaggcga caatgatgaa ttcaaagata tgaaagcgtc actacgcgcc
     2521 agttatcagc ataataccga gaacggtcgt ctctatctct ccgggacatc acagcgagac
     2581 agttattatt ctctgaatgc cagttggaat ggttcattca cggcgactcg tcacggagcc
     2641 gctttccatg actatagtgg tagtgctgac tcgcgtttta tgatcgacgc agatggtgca
     2701 gaagatattc cgttgaacaa taaacgcgcg gtaactaatc ggtatggcat cggagttatt
     2761 ccatcagtca gcagttacat aacaacatca ttaagcgttg atacccgaaa tcttccagaa
     2821 aatgtggata tcgaaaactc ggttatcacc accaccttaa ccgaaggtgc tattggctac
     2881 gccaaacttg atacccgcaa gggctatcaa atcatggggg ttattcgcct ggcagatggt
     2941 agtcacccac cactggggat tagcgtaaaa gataaaacca gccataaaga attaggactg
     3001 gttgctgatg gcggctttgt atacctcaac ggcattcagg atgacagcaa acttacttta
     3061 cgctggggtg ataaatcctg ttttattcaa ccacccaata gcagcaactt aaccaccgga
     3121 acggttattt taccgtgtat tagccaaaat taattcagga tgaaactaat gcgctactta
     3181 aacacaaaga atctcattgc agcaggagta ttactcgcct gcatgagcag cattgcctgg
     3241 ggtgccatca tcccggaccg tacccgtatc ataatgaatg agtcagataa aggggaagca
     3301 ttaaaactta ctaatcaaag caaaaatcta ccctatctgg cgcaaacatg gatagaagat
     3361 acgaaaggca ataaatcacg taactttatt gtcacggttc cccctatggt acgtttaaat
     3421 cccagtgagc aaattcaaat aagaatgata agtcaggaaa aaattgctca actgcctaat
     3481 gacagagaaa cgctcttcta ttttaacgtg cgagaaatac cgccaaaaac agataaaaaa
     3541 aatgtgatgc aagtcactat gcagcacgca ttgaaactat tctggcgacc aaaagctatt
     3601 gaattagaag atgatggggt aatgtcttac gaaaaagttg aaatcatccg tagaaatgat
     3661 ggttcgatcc gctttaacaa taagatgcct tatcacgtta cgctgggtta tatcggaaca
     3721 aacggaatca caatgttgcc acaaacgcaa agtttaatgg taacccctta tagccatgca
     3781 gatacgcagt ttaaaaatgt tccgtcagct tttcaggttg gttatatcaa tgattttggt
     3841 ggattaagtt tttatgaaat aaactgcccg acggttaata actcttgtaa tgtttccgta
     3901 gccaaacgag ataaa
//
LOCUS       DAPHDX010000236         3877 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPHDX010000236 DAPHDX010000000
VERSION     DAPHDX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.239"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..3784
                     /locus_tag="RH058_004388"
                     /pseudo
     CDS             5..3784
                     /locus_tag="RH058_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          846 a         1037 c         1380 g          614 t
ORIGIN      
        1 gagaatgagc ggaaaaccgg cggcgcggca gggcgacatg acccgcaagg gactggatat
       61 tgtgcagggt tcagcagggg tgctgatagg cgcgccgacg ggggttgcct gctcggtgtg
      121 tcctaaaaaa aaggacagtc caaattatgg caacccggtg aagccggtgc tgggtgcaaa
      181 ggtgctgccg ggcgagacgg acattgcgct gcccggcccg ctgccgttta ttctttcccg
      241 cgcctacagc agctaccgga ccagaacacc cgcgccggtg ggggtgtttg gtcccggctg
      301 gaaagcgccc ttcgatatcc gcttacagat acgcgatgaa ggcctgatac tcaacgacag
      361 cggcggcagg agcattcact ttgagccgct gtttcccggc gagataagct acagccgcag
      421 cgagtcactc tggctggcgc ggggcggagt agcggcgcag cacagttcgc agccgctaag
      481 cgcgctctgg caggtgctgc cggaagatgt tcgcctgagt ccgcatgtgt acctggcgac
      541 aaacagcctt caggggccgt ggtggatact cagctggccg gagcgggtgc cgggggcgga
      601 cgaggtgctg ccgccggagc cgcccgcata ccgggtgctg acgggcgtgg tggacggctt
      661 cgggcggacg ctggcctttc accgggcggc ggagggtgat gtggcgggcg cggtgacggg
      721 ggtgacggac ggcgcgggcc gccgttttca cctggtgctg accacacagg cacagcgggc
      781 ggaagtattc cgtaaacagc gcgccacgtc tttatcttcc cctgccggtc cccgttctgc
      841 ttcctcttct ctggttttcc ccgacacgct gcccgccggt acaggatacg gcacggataa
      901 cggtatccgg ctggaggcgg tatggctgac acacgacccg gcatacccgg atgaacagcc
      961 gactgcgccg ctggcgcgct acacgtacac ggccggtggt gaactgcggg cggtgtatga
     1021 ccgcagcgga acgcaggtgc gcgggtttac ttatgatgcg gagcacgccg ggcggatggt
     1081 ggcgcaccat tatgcgggga ggccggagag ctgctaccgg tatgatgata ccggtcgggt
     1141 gacggagcag gtcaacccgg aggggctgga ctaccgcttt gagtacggag agagccgggt
     1201 aatcatcacg gacagcctga accggcggga ggtgctgtac acggaaggcg agggtggcct
     1261 gaagcgggtg gtgaagaagg aacatgcgga cgggagcatt acccgcagcg agtatgatga
     1321 ggcggggagg ctgaaggcac agacggatgc ggcggggcgg cggacggagt acagcctgca
     1381 tatggcgtcg ggtgcggtga cggcggtgac ggggccggac ggcaggacgg tacggtatgg
     1441 ctataacagc cagcggcagg tgacgtcggt gacgtacccg gacgggcttc gcagcagccg
     1501 ggagtatgat gagaagggaa ggctgacggc ggagacctcg cgcagcggtg agacaacgcg
     1561 gtacagctat gatgacccgg cgagtgagct gccgacaggg atacaggacg cgacgggcag
     1621 tacaaaacag atggcatgga gccgttacgg tcaactgctg gccttcacgg actgctcggg
     1681 gtacacgacg cggtatgagt acgaccggta cggtcaacaa atcgccgttc accgggagga
     1741 aggcatcagc acttacagca gttataaccc gcgtgggcaa ctggtcagtc agaaggatgc
     1801 gcaggggcgt gaaatccgtt atgagtacag cgccgcaggc gacctgacag caacgatatc
     1861 accggacggt aaacgcagca ccatcgaata tgataaacgc gggcgtccgg tgagtgtgac
     1921 ggaaggcggt cttacgcgca gcatggggta tgacgctgcc gggcgcatca ccgtgctgac
     1981 caacgagaac ggcagccagt ccacgttccg gtatgacccg gtggacaggc tgactgaaca
     2041 gcgcggtttt gacggccgga cgcaacgtta ccactatgac ctgaccggaa aactcacgca
     2101 gagtgaagac gaggggctta tcaccctctg gcactacgat gcgtcggacc gcatcacgca
     2161 ccggacggtg aacggcgacc cggcagagca gtggcagtat gatgagcacg ggtggttaac
     2221 caccctcagc catacctgtg aaggccaccg cgtgtcggtc cactacggct atgacgataa
     2281 aggccgcctg acgggtgaac ggcagacggt ggagaacccg gagacagggg agatgctgtg
     2341 ggagcatgag acggggcatg cgtacagcga acaggggctg gcgacccgtc aggagccgga
     2401 cggtctgccg ccggtggagt ggctgacgta tggcagcggt tatcttgcgg ggatgaagct
     2461 gggcggaacg ccgctggtcg agtacacgcg ggaccggctg caccgcgaga cggcccgtag
     2521 cttcggcggg gcgggcagta cagcggggta cgaacaggcc acggcgtaca ccctcacagg
     2581 gcagctacag agcaggcacc tgaaccttcc gcagcttgac tgtgactata cgtggaacga
     2641 taacggacaa ctggtgcgca tcagcggccc gcaggagtgc cgggagtacc gttacagcgg
     2701 cacgggaagg ctgacgggcg tccacactac ggcagcgaac ctggatatcg atatcccgta
     2761 tgcgacggac ccggcaggaa accggctgcc ggacccggaa ctgcacccgg acagcaccct
     2821 cacggcgtgg ccggataacc gcatcgcgga agatgcgcac tatgtctacc gctacgatga
     2881 atacggcagg ctggcggaga agacggaccg catcccggaa ggggtgatac ggatgcacga
     2941 cgagcggacc caccactacc actacgacag ccagcaccgt ctggtgttct acacgcggat
     3001 acagcacggt gagccgcagg tggagagccg ctacctctat gacccgctgg gccgccggac
     3061 gggaaaacgg gtgtggcggc gggagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
     3121 accggaggag acctggtacg ggtgggacgg tgacaggctg accactgtac agacccaaca
     3181 gacaagaatc cagacggtat accagccggg aagcttcacg ccgctcctga gaatcgaaac
     3241 agagaatggt gaacaggcga aggcgcggca ccgtagcctg gcggaggtgt tgcaggagga
     3301 cacgggtgtg acgctaccgg cggagctggc ggtgatgctg gggaggctgg agcgggaact
     3361 gcggcagggc agcgtgagtg aagaaagcca gcagtggctt gcgcagtgcg ggctgacggc
     3421 ggaacagatg gccgcgcaac tggaggcgga atacatcccg gagaggaaac ttcatcttta
     3481 ccactgtgac caccgtgggc tgccgctggc gcttatcagc ccggaagggg aaacggcgtg
     3541 gcagggggag tatgacgagt ggggaaacct gctgggcgag gaaagcgcgc agcaccttca
     3601 gcagtcactc cgtctgccgg gacagcagta tgatgaggag tcggggctgt actacaaccg
     3661 caaccggtac tatgatccgt tgcaggggag atatatcacc caggacccga tagggctgag
     3721 aggtgaatgg aatctgtata aatatccatt aaaccctgtt cggtttatag attctttggg
     3781 tttaaaattc catgttaacg gtgatccaag tgattttaat caggctgttg agtatttaaa
     3841 acaagattca caaatgaaag aaactataga tttttta
//
LOCUS       DAPHDX010000237         3598 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPHDX010000237 DAPHDX010000000
VERSION     DAPHDX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.240"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..673
                     /gene="fimC"
                     /locus_tag="RH058_004389"
     CDS             <1..673
                     /gene="fimC"
                     /locus_tag="RH058_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV3139859.1"
                     /translation="LMLIIFYLIISASTHAAGGIALGATRIIYPADAQQTAVWIRNSH
                     TNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWM
                     NVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINV
                     HNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVR
                     EVNLN"
     gene            704..3313
                     /locus_tag="RH058_004390"
     CDS             704..3313
                     /locus_tag="RH058_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV3139860.1"
                     /translation="MKIPTTTDIPQRYSWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPARVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3326..>3598
                     /locus_tag="RH058_004391"
     CDS             3326..>3598
                     /locus_tag="RH058_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3139861.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQET"
BASE COUNT          946 a          796 c          873 g          983 t
ORIGIN      
        1 attgatgctc attatatttt atttaatcat ttcggccagc acccatgctg ccggagggat
       61 cgcattaggt gccacgcgta ttatttatcc cgctgatgct caacagactg cggtatggat
      121 tagaaatagc cataccaatg agcgctttct ggtcaattcg tggattgaaa acagcagcgg
      181 tgtaaaagaa aagtcattca tcattacacc gccactgttt gttagtgaac ccaaaagcga
      241 aaataccttg cgtattattt acaccggtcc accgctggcg gcagatcgtg agtctctgtt
      301 ctggatgaat gttaagacga tcccttcggt agataaaaat gcattgaacg gcaggaatgt
      361 tttgcaactg gcgattttat cgcgcatgaa attatttctc cgtccaattc aattacaaga
      421 attacccgca gaagcgccgg acacactcaa gttttcgcga tccggtaact atatcaatgt
      481 tcataatccc tcaccttttt atgtcaccct ggttaactta caagtgggca gccaaaagtt
      541 ggggaatgct atggctgcac ccagagttaa ttcacaaatt cccttaccct caggagtgca
      601 gggaaagctg aaatttcaga ccgttaatga ttatggttca gtaaccccgg tcagagaagt
      661 gaacttaaac taaccgaatc atctgacaat atcagagcta attatgaaaa tacccactac
      721 tacggatatt ccgcagaggt atagctggtg tctggccgga atttgttatt catctcttgc
      781 cattttaccc tcctttttaa gctatgcgga aagttatttc aacccggcat ttttattaga
      841 gaatggcaca tccgttgctg atttatcgcg ctttgagaga ggtaatcatc aacctgcgcg
      901 cgtgtatcgg gtggatctct ggcgtaatga tgagttcatt ggttcacagg atatcgtatt
      961 tgaatcgaca acagaaaata caggtgataa atcaggtggg ttaatgccct gttttaacca
     1021 ggtactcctt gaacgaattg gccttaatag cagtgcattt cccgagttag cccagcagca
     1081 aaacaataaa tgcatcaatt tactgaaagc tgtacctgat gccacaatta actttgattt
     1141 tgcagcgatg cgcctgaaca tcactattcc tcagataacg ttgttgagta gcgctcacgg
     1201 ttacattccg cctgaagagt gggatgaagg tattcctgct ttactcctga attataattt
     1261 caccggtaac agaggtaatg gtaacgatag ctattttttt agtgagctca gcgggattaa
     1321 tattggcccg tggcgtttac gcaacaatgg ttcctggaac tattttcgcg gaaatggata
     1381 tcattcagaa cagtggaata atattggcac ctgggtacag cgcgccatta ttccgctgaa
     1441 aagtgaactg gtaatgggag acggcaatac aggaagtgat attttcgatg gtgttggatt
     1501 tcgtggtgta cggctttatt cttccgataa tatgtatcct gatagccagc aagggtttgc
     1561 cccaacggta cgtgggattg cccgtacggc ggcccagcta acgattcggc aaaatggttt
     1621 tattatctat caaagctatg tttcccccgg cgcttttgaa attacagatt tgcacccgac
     1681 atcttcaaat ggcgatctgg atgtcaccat cgacgagcgc gatggcaatc agcagaatta
     1741 cacaattccg tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct
     1801 gacggcgggc gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg
     1861 cacggcactc ggcggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc
     1921 caattacacc gcctttttat taggactggg gcgcaatctc ggaaactggg gggcagtgtc
     1981 gctggatgtg acccatgcgc gcagtcagtt agccgacgac agtcgtcatg agggggattc
     2041 cattcgcttc ctctatgcga aatcgatgaa caccttcggc accaattttc agttaatggg
     2101 ttaccgctat tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga
     2161 ggggtacgaa tatgattacg attacgacgg tgagcatcgg gatgaactga taatcgtgaa
     2221 ttaccacaat ttacgcttta gccgtaaaga ccgtttgcag ttaaatattt cacaatcact
     2281 taatgacttt ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga
     2341 ttcagatacc tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcgct
     2401 ctcattttcg tggaatgaat ctgtagggat ccccgataac gaacgtattg tcggacttaa
     2461 tgtttcagtg cctttcaatg ttctgaccaa acgtcgctac acccgggaaa atgcgctcga
     2521 ccgcgcttat gcctccttta acgccaaccg taacagcaac gggcaaaata gctggctggc
     2581 aggtgtaggt gggaccttac tagaaggcca caacctgagt tatcacgtaa gccagggtga
     2641 tacctcgaat aatgggtata cgggcagcgc cacggcaaac tggcaggccg cttacggtac
     2701 gctgggggtc gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg
     2761 cggtgtggtc gggcacgaaa atggcataac gctgagccag cctttagggg ataccaatgt
     2821 tttgattaaa gcgcctggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac
     2881 cgactggcgc ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc
     2941 gcttgatacc aatacgatgg ggaattccat cgatgttgaa aaaaatatca gcagcgttgt
     3001 gccgacgcaa ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct
     3061 cattaccgtt acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag
     3121 taccggaata accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt
     3181 gtctggtgaa ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta
     3241 tgtattgccg aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca
     3301 tcctggctca taaaggaaat tatcaatgaa gataatctgc agattattat tggcgatggc
     3361 atgtttgtgt ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc
     3421 agaagatgaa cactatgatc tctcaaatat ctttaatagc accaataacc agccagggca
     3481 gattgttgtt ttaccggaaa aatccggctg ggtaggtgtg tcagcaattt gtccacccgg
     3541 cacgctggtg aattatacat accgtagtta tgtcaccaac tttattgttc aggaaact
//
LOCUS       DAPHDX010000238         3579 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPHDX010000238 DAPHDX010000000
VERSION     DAPHDX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.241"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..297
                     /locus_tag="RH058_004392"
     CDS             <1..297
                     /locus_tag="RH058_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3139862.1"
                     /translation="KQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKWYFQRLFK
                     KVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRVMTPTY"
     gene            complement(291..563)
                     /locus_tag="RH058_004393"
                     /pseudo
     CDS             complement(291..563)
                     /locus_tag="RH058_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            687..2090
                     /locus_tag="RH058_004394"
     CDS             687..2090
                     /locus_tag="RH058_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDV3139863.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTSAEEELLRKMLPEAPAHHPNYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(2124..3338)
                     /locus_tag="RH058_004395"
     CDS             complement(2124..3338)
                     /locus_tag="RH058_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HDV3139864.1"
                     /translation="MFMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLYIPS
                     GIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIYYNSRHFGKTRNEKRITRW
                     GRGSPLQNPENTGALTLLAFKLDEQGGDCKEVNIWVCASTDEEDVIETAIGEVIPGAL
                     ISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEIIQYAASHYVKNSLDPDEQL
                     LDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSVSNRRKSRAGKSLELHLEH
                     LFIEHGLRHFSTQAITEGNKKPDFLFPSAGAYHDTEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSLHKKYPEAVRAELMTLGAF
                     IAELTELYADIP"
BASE COUNT          982 a          836 c          845 g          916 t
ORIGIN      
        1 aagcagtttc atacaacggt tatcaaagat gttctattat ggattgaaca taatttagat
       61 cagtctttac tgcttgatga tgtggcgaat aaagcgggtt ataccaagtg gtattttcag
      121 cggctgttca aaaaagtaac aggggtcaca ctggctagct atattcgtgc tcgtcgtttg
      181 acgaaagcgg ctgttgagtt gaggttgacg aaaaaaacta tccttgagat cgcattaaaa
      241 tatcaatttg attcccaaca atcttttaca cgtcgggtaa tgactccaac ttactgatag
      301 tgttttatgt tcaaataatg cccgatgacc ttgtcatgca actccaccga ttttgagaac
      361 gacagtgact tccgtcccag ccttgccaga tgttgtctca gattcaggtt atgtcgctca
      421 atgcgctgag tgtaacgctt gctgataacg tgcagctttc ccttcaggcg ggattcatac
      481 agcggccagc catccgtcat ccataccacg acctcaaagg ccgacagcag gcccagaaga
      541 cgctccagcg tggccaacgt gcgttaccta aaacctgttt aaatatccag ataaaaacat
      601 tcaatctggg tcaaatgagt gatacagttt cacccataag acccaatgga ggcaatatgt
      661 ctgaatttga attactggcg caggatctgc ttgagaaagc agaagcggaa gaacaactgc
      721 gacaggaaaa tgataaaaag ctgctcgggc aggtgctgga aatctatgac cagaagtacg
      781 tggctgaact gcttagaaaa gttggtaaaa atgagtggag tcgcgagact cttaatcgct
      841 ggattaatgg taagtgctca cctaagacgc tgacgtcagc cgaagaggaa cttctacgaa
      901 aaatgcttcc ggaagcgcct gcacatcacc ctaactatgc cttccggttt attgacctgt
      961 ttgctgggat tggaggtata cggaagggct tcgaaaccat cggtggccag tgcgttttta
     1021 ccagtgaatg gaataaagag gctgtgcgca catataaagc taactggttt aacgatgctc
     1081 aggaacacac tttcaatctc gatattcggg aagtcacgct cagtgataaa cctgaagtac
     1141 ctgaaaacga tgcctatgct tacattaatg agcatgtgcc ggatcatgat gtacttctag
     1201 caggtttccc ctgtcaaccg ttcagccttg cgggcgtaag caagaaaaac tcgctcgggc
     1261 gcgcgcatgg tttcgaatgt gaggctcagg gaacactttt cttcgatgtg gcgcgtatta
     1321 tccgcgcaaa aaaacctgcc atctttgttc ttgaaaacgt taaaaacctg aagagccatg
     1381 acaagggtaa aacctttaaa gtcatcatgg ataccctcga cgaactgggc tatgaagttg
     1441 cggatgcagc tgagatgggc aaaaacgatc ctaaagttat cgacggaaag cactttttac
     1501 ctcagcaccg agaacgtatc gttttggtcg gtttccgtcg tgatctgaac attcaccagg
     1561 gctttaccct gcgcgatatt agtcgttttt atccggaaca gcgtccgtca tttggcgaac
     1621 tgctggaacc cgtggttgac agcaaatata tactgacgcc gaaactctgg gagtatctct
     1681 ataactacgc caaaaagcac gcagctaagg gtaacggatt cggttttggc ctcgttaatc
     1741 ctgaaaataa agaaagcatt gcccgtacgc tttctgctcg ctatcacaaa gacgggtctg
     1801 aaattctgat agaccgtggc tgggatatgg ccacaggtga aacagacttc gcgaacgaag
     1861 aaaatcaggc gcatcggccc cgtaggctga ctccacgaga gtgcgcgcgc cttatgggtt
     1921 ttgaaaaagt agatggcagg ccttttcgca ttcctgtgtc agacactcag tcgtacaggc
     1981 agttcggtaa ctccgtagtg gtgcccgtgt ttgaagccgt agccaaactg cttgaacctt
     2041 atatcctgaa agcggttaat gccgattcgt gcaaggttga acgaatctga tcgctcctcc
     2101 cggtatttat gccgggagat aatctatgga atatctgcgt aaagctctgt cagctcagca
     2161 ataaacgcac ctagcgtcat tagctcagct ctcaccgcct ccgggtattt tttgtgcaga
     2221 gatgatggca cgaccaatct gacacccgac tcccgcatct cccgatattg agccagagaa
     2281 actccctcct ggagtgtaaa cagatgcacc tgatgaattt tatcggcctc attcagtatc
     2341 tgacgccagc gatccttaca ggtagtcttg actgccagca tgcgcagatt ttctacggga
     2401 aactcagtat cgtggtaagc ccctgcggaa gggaaaagga aatcgggttt tttattacct
     2461 tctgtgatgg cctgcgtcga aaagtgtcgc aggccgtgct caatgaatag atgctccagg
     2521 tgcagttcca gcgacttccc ggctctggat ttacggcgat tgctgacaga attggccagc
     2581 gcaataaatt catccacaga gccaaatcct ttccggatga tatccagaac atgcagttcc
     2641 tcaaccaata gaaatatgtc gtactccacg cgccggcggt caagaagttg ctcatccgga
     2701 tcaagggaat ttttcacata atggctggct gcatactgaa taatttcact tcccgacgga
     2761 aagcgcaggt gccagtcttc aggtagaata tatttatgat ttactggcgc ttgctgtaga
     2821 gatagtccgc ctagaatctg tcctgcgggg ccggatataa gcgctccggg tataacttca
     2881 ccaatagcgg tctcaatgac gtcctcttca tcagtgctgg cgcataccca aatatttact
     2941 tccttacagt cccccccttg ctcatcaagc ttgaaagcta ggagcgtcag agcacctgta
     3001 ttttcaggat tctgaagtgg gctgcctcta ccccagcggg taatcctttt ttcattccgg
     3061 gttttaccaa aatgacggct gttataataa attgcccggg cttcgctgtc agggcaatca
     3121 tgcgatgaca catgtgcggt gagaaaaacc gaagggttca gttcacgggt atggttgata
     3181 gacggaaaga gtttttcaac gatacctgaa gggatataaa gtcctacctg gtgaccacct
     3241 gttgcgccgg tatcgttggc ggaaaggcgt ttgatgtaga cgaagtaatt ctcacatgcg
     3301 atctcgagta gccagttgtg gaaaaccgac ataaacatcc cctgttaccc tgaaactcta
     3361 ctcaccattt tttcataatt atatacaaac agccagaaag gctgttacag gcgatttgat
     3421 ctgcaaccta ttggttaaat taatgtatca aaaacgatgg tttctgtgac acatccctat
     3481 aagcagttgc ctatacaata aaacgctctc cggctgtaac aaaacccgtt acagtatcaa
     3541 tacaacaaaa acgatgtaac aaaatagggt ggcaaaatg
//
LOCUS       DAPHDX010000239         3560 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPHDX010000239 DAPHDX010000000
VERSION     DAPHDX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.242"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1914
                     /gene="ag43"
                     /locus_tag="RH058_004396"
     CDS             <1..1914
                     /gene="ag43"
                     /locus_tag="RH058_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3139865.1"
                     /translation="KAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGG
                     TLAGTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSN
                     TTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGAT
                     WNIPDNATVLSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMA
                     QNNADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQG
                     NKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTG
                     VSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTA
                     GVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVALGTRHSMK
                     ASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKF
                     GHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFS
                     SRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSA
                     EGYNGQATLNVTF"
     gene            2035..>3560
                     /locus_tag="RH058_004397"
     CDS             2035..>3560
                     /locus_tag="RH058_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139866.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     G"
BASE COUNT          850 a          956 c          995 g          759 t
ORIGIN      
        1 aaggcattca gtatcggagg cggtcaggcg gatgccctga tgctggaaaa aggcagttca
       61 ttcacgctga acgccggtga tacggccacg gataccacgg taaatggcgg actgttcacc
      121 gccaggggcg gcacactggc gggcaccacc acgctgaata acggcgccat acttaccctt
      181 tccgggaaga cggtgaacaa cgataccctg accatccgtg aaggcgatgc actcctgcag
      241 ggaggctctc tcaccggtaa cggcagcgtg gaaaaatcag gaagtggcac actcactgtc
      301 agcaacacca cactcaccca gaaagccgtc aacctgaatg aaggcacgct gacgctgaac
      361 gacagtaccg tcaccacgga tgtcattgct cagcgcggta cagccctgaa gctgaccggc
      421 agcactgtgc tgaacggtgc cattgacccc acgaatgtca ctctcgcctc cggtgccacc
      481 tggaatatcc ccgataacgc cacggtgctg tcggtggtgg atgacctcag ccatgccgga
      541 cagattcatt tcacctccac ccgcacaggg aagttcgtac cggcaaccct gaaagtgaaa
      601 aacctgaacg gacagaatgg caccatcagc ctgcgtgtac gcccggatat ggcacagaac
      661 aatgctgaca gactggtcat tgacggtggc agggcaaccg gaaaaaccat cctgaacctg
      721 gtgaacgccg gcaacagtgc gtcggggctg gcgaccagcg gtaagggtat tcaggtggtg
      781 gaagccatta acggtgccac cacggaggaa ggggcctttg tccaggggaa taagctgcag
      841 gccggtgcct ttaactactc cctcaaccgg gacagtgatg agagctggta tctgcgcagt
      901 gaaaatgctt atcgtgcaga agtccccctg tatgcctcca tgctgacaca ggcaatggac
      961 tatgaccgga ttctggcagg ctcccgcagc catcagaccg gtgtaagcgg tgaaaataac
     1021 agcgtccgtc tcagcattca gggcggtcat ctcggtcacg ataacaacgg cggtattgcc
     1081 cgtggagcca cgccggaaag cagcggcagc tatggcttcg tccgtctgga gggtgacctg
     1141 ctcagaacag aggttgccgg tatgtctgtg accgcggggg tatatggtgc tgctggccat
     1201 tcttccgttg atgttaagga tgatgacggc tcccgtgccg gcacggtccg ggatgatgcc
     1261 ggcagcctgg gcggatacct gaatctgaca catacatcct ccggcctgtg ggctgacatt
     1321 gtggccctgg gaacccgcca cagcatgaaa gcgtcaacgg acaataacga cttccgcgcc
     1381 cggggttggg gctggctggg ttcactggaa accggtctgc ccttcagtat cactgacaac
     1441 ctgatgctgg agccacaact gcagtatacc tggcagggac tttccctgga tgacggtaag
     1501 gacaacgccg gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggtttc
     1561 cgtctgggca gccacaacga tatgaccttt ggcgaaggca cctcatcccg tgcccccctg
     1621 cgtgacagtg caaaacacag tgtgagtgaa ttaccggtga actggtgggt acagccttct
     1681 gttatccgca ccttcagctc ccggggagat atgcgtgtgg ggacttccac tgcaggcagc
     1741 gggatgacgt tctctccctc acagaatggc acatcactgg acctgcaggc cggactggaa
     1801 gcccgtgtcc gggaaaatat caccctgggc gttcaggccg gttatgccca cagcgtcagc
     1861 ggcagcagcg ctgaagggta taacggtcag gccacactga atgtgacctt ctgacagaac
     1921 catcgcctct ctgtggtccc ggtcatcatg accgggaccc ggaccggcgc aacggatctt
     1981 caacgccaca ttcgctggca ttaacaataa catgatattc atcacggagt gactatgtta
     2041 cagatagtcg gcgcgctgat cctgctgatc gcaggatttg ccattcttcg ccttttgttc
     2101 agagcattaa tcagcacggc ttctgcgctg gcagggctca tattgctgtg tctgttcggc
     2161 ccggccttac tggctggcta tatcaccgaa cgcataaccc ggttgttcca tattcgctgg
     2221 ctggcaggcg tatttctgac gattgccgga atgatcatca gcttcatgtg gggacttgat
     2281 ggtaaacata tcgcgctgga ggctcacacc tttgactctg tgaaatttat tctgaccact
     2341 gctctcgccg gtggtctgct ggctgttccc ctgcagatca aaaacattca gcagaacggg
     2401 atcacaccag aagatatcag caaggaaatt aacgggtatt actgctgttt ttatactgcc
     2461 tttttcctta tggcgtgttc tgcatgcgca ccattgatcg cgttacagta cgatatttca
     2521 ccgtcactga tgtggtgggg cgggttgttg tactggctgg ctgcattagt gacgctgcta
     2581 tgggcggcca gccagatcca ggcgctgaaa aaactgacct gtgccatcag ccagacactg
     2641 gaagaacaac cggtgctcaa cagtaaatcg tggctgacca gtttgcaaaa cgattacagc
     2701 cttcctgact cactgacgga gcgcatctgg ctgacgctca tttctcaacg gatttcccgg
     2761 ggagagctga gggaatttga actggcagac ggaaactggt tactgaacaa tgcctggtat
     2821 gaaagaaaca tggcagggtt taacgaacag ttgaaagaga acctgtcatt cacacctgat
     2881 gaactgaaaa cgctcttccg gaaccgcctg aatttatcac cggaagcgaa tgacgatttt
     2941 ctcgatcgtt gcctggacgg cggtgactgg tatccctttt cagaaggtcg ccgttttgta
     3001 tcattccatc acgtggatga gcttcgtatc tgtgcctcct gcgggctgac agaagtacat
     3061 catgccccgg aaaatcataa gccggatccg gaatggtact gctcctctct ttgtcgcgaa
     3121 acagaaacac tgtgtcagga aatttatgaa cgcccttaca acagctttat ttccgatgca
     3181 acggcgaatg gtctgattct catgaaactg ccggaaacct ggagtacaaa tgagaaaatg
     3241 tttgcttccg gagggcaggg acatgggttt gccgctgaac ggggaaacca tattgtcgac
     3301 agagtccgtc tgaaaaacgc acggatcctc ggtgataata atgccagaaa tggagcagac
     3361 agactggtca gcggaacaga aatccagacg aaatattgtt caactgcagc ccgtagcgtc
     3421 ggtgcggcat tcgacggaca aaacggacag tatcgttaca tgggaaataa tggccccatg
     3481 caactggaag tcccccgtga tcagtatgcc ggcgctgttg aaaccatgag gaataagatc
     3541 cgcgaaggta aagtacccgg
//
LOCUS       DAPHDX010000240         3462 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPHDX010000240 DAPHDX010000000
VERSION     DAPHDX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.243"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1042
                     /locus_tag="RH058_004398"
     CDS             <1..1042
                     /locus_tag="RH058_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDV3139867.1"
                     /translation="LAGRPMMDTLVNQHSDINKTHEILQDESKCEMIVIIENFMTSSA
                     KYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLG
                     PDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAF
                     RDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPERRT
                     FPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGE
                     VRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
                     NLVEIEKIVSVN"
     gene            1113..1538
                     /locus_tag="RH058_004399"
     CDS             1113..1538
                     /locus_tag="RH058_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3139868.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1535..1885
                     /gene="tnpB"
                     /locus_tag="RH058_004400"
     CDS             1535..1885
                     /gene="tnpB"
                     /locus_tag="RH058_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3139869.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            1916..>3462
                     /locus_tag="RH058_004401"
     CDS             1916..>3462
                     /locus_tag="RH058_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HDV3139870.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLS
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVL"
BASE COUNT          914 a          868 c          924 g          756 t
ORIGIN      
        1 cttggctggt cggcccatga tggataccct cgttaatcag cattctgaca tcaacaaaac
       61 gcatgaaatt ctgcaggacg aatcgaaatg cgaaatgatt gtgatcatcg aaaactttat
      121 gacctcatcg gcaaaatatg ccgacattct gctgccagac ctgatgaccg ttgagcagga
      181 agatattatt cctaacgact acgccggtaa catgggatat ctcattttcc tccagcctgt
      241 caccagcgaa aaattcgaac gcaaaccgat ttactggatc ctgagtgaag tcgcgaaacg
      301 tcttggacct gacgtctatc aaaagttcac agaaggtcgc acgcaggaac aatggttaca
      361 acatctgtac gccaaaatgc ttgccaaaga tccggcgtta ccgtcttacg acgaactgaa
      421 aaaaatgggt atctataagc gtaaagatcc caatggccat tttgtcgcct acaaagcatt
      481 tcgtgacgac cccgaggcaa atccacttaa aacgccttcc ggtaagattg aaatttattc
      541 cagcaggctg gcggaaattg cccgtacctg ggaactggaa aaagatgaag tgataagccc
      601 attgccagtt tatgcctcaa cctttgaagg ctggaactcc cctgaacgta gaaccttccc
      661 actgcaactg tttggtttcc attacaaatc ccgtactcac tcgacctacg gcaatattga
      721 tctcctgaag gctgcctgcc gtcaggaggt gtggatcaac cctatagatg cgcagaaacg
      781 tgggattgcc aacggcgata tggtgcgggt gtttaaccat cgtggcgaag ttcggctacc
      841 agccaaagta acaccacgta ttctccctgg agttagcgct atgggccagg gagcctggca
      901 cgaggccaat atgtctggcg ataaaatcga ccatggcggc tgtgtgaata cgctaaccac
      961 tctgcgccct tcaccactgg cgaagggaaa cccgcagcac actaatctgg tcgagatcga
     1021 aaaaatagta agcgtaaact gaccgccgta tgtagccatc agacgagaat tggtaactta
     1081 gacgcccatc tgatatagac ggacatctaa gtatggaatt acaggactgg cgaaaagaac
     1141 ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt ggaactggca tcacaacctg
     1201 gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa tgataatgtt attttcaaat
     1261 ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg tcttccggta acgacctctt
     1321 ctgacactgg cgttgaatta ttacctgtag aaataacgcc ggatgagcag aaagaacctg
     1381 tggcggccat tgcgccgtct ttatccactt ccactcagac cagagtcagt gccagttcct
     1441 gcaaggtgga attccgtcac ggtaacatga cgctggaaaa tccatcgcca gagctgctca
     1501 cagtgttgat ccgtgaactg accgggaggg gaagatgatc tcactcccat caggtacccg
     1561 tatctggctc gttgccggcg ttaccgatat gcgtaaatcc ttcaacggac tgggagaaca
     1621 ggtacaacat gtgctgaatg ataatccctt ctccggtcac ctgtttatct tccgtggccg
     1681 acggggtgac accgtcaaaa ttctttgggc tgatgctgat ggtctgtgcc tgttcaccaa
     1741 acgcctggag gaaggccagt ttatctggcc tgcggtacgt gacggcaagg tatccattac
     1801 ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg cgtcagccaa aaacatccag
     1861 ccgtaactca ctgacaatgt tgtaaaaaac tcctgaccgc attataaaaa cggtcatgag
     1921 tcagaaatac ctcattcgca tcgcagagct ggaaaggttg ctctctgagc aggctgaagc
     1981 cctccgtcag aaagaccagc aactgagtct ggttgaagag acggaagcct tcctgcgctc
     2041 tgcactgaca cgtgccgaag aaaagatcga agaagatgaa cgggaaatag aacatctgcg
     2101 ggctcagata gaaaaactgc gccggatgct gttcggtacc cgttctgaaa aactgcgtcg
     2161 tgaagttgaa ctggctgagg ctctgctgaa acaacgtgaa caggacagcg atcgttacag
     2221 tgggcgggaa gacgatcctc aggttccccg ccagttgcga cagtcgcgcc atcgtcgtcc
     2281 gttaccggca caccttcccc gtgaaataca ccgcctggag ccagaagaaa gctgttgccc
     2341 ggagtgtggc ggtgagctgg attatctggg ggaagtcagc gctgaacagc tggaactggt
     2401 gagcagtgcc ctgaaagtga tccgcacaga acgggtaaaa aaagcctgta caaaatgtga
     2461 ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag cgtggtatcg cgggccccgg
     2521 attacttgcc cgcgtgttaa cgggaaaata ctgcgaacat ctgccactgt atcgtcagag
     2581 tgaaatcttt gcccgccagg gtgtcgaact gagccgggcc ttactctcca actgggttga
     2641 cgcgtgctgc cagttaatga caccggtgaa tgatgccctg taccgttatg taatgaacac
     2701 ccgcaaggtt cacactgatg acacaccggt aaaggtactg tcaccgggtc agaaaaaggc
     2761 gaaaacaggg cgtatctgga cgtatgtccg ggatgatcgc aatgtgggtt cgtcatctcc
     2821 tccagcggtc tggttcgcgt actcgccgaa ccggcagggg aaacacccgg agcaacacct
     2881 ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca ggttacgaca ggttgttcag
     2941 tgcagaacgt gaaggtggtg cactgacaga agttgcgtgc tgggcccatg cccggcgaaa
     3001 aatccacgat gtatacatca gcagcaaaag tgcgacggca gaagaagccc tgaagcgaat
     3061 cagtgaactg tacgccatcg aggatgaaat acggggatta ccggagtcag agcgtcttgc
     3121 cgtcaggcag cagcgaagca aagtgttact gacgtcgctg catgaatgga tggtggagaa
     3181 gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg ttcagctatg tactgaatca
     3241 gtgggatgcc ctctgttatt acagtgatga cggtctggcg gaggcggata ataatgctgc
     3301 ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac tttatgttct ttggcagcga
     3361 tcacggcggc gagcgtggag cactgttgta cgggctgatc ggcacctgcc gtctgaacgg
     3421 tatcgatccg gaagcgtatc tgcgccatat cctgagcgta ct
//
LOCUS       DAPHDX010000241         3229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPHDX010000241 DAPHDX010000000
VERSION     DAPHDX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.244"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(85..612)
                     /gene="mobB"
                     /locus_tag="RH058_004402"
     CDS             complement(85..612)
                     /gene="mobB"
                     /locus_tag="RH058_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV3139871.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(594..1178)
                     /gene="mobA"
                     /locus_tag="RH058_004403"
     CDS             complement(594..1178)
                     /gene="mobA"
                     /locus_tag="RH058_004403"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV3139872.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1248..1517
                     /locus_tag="RH058_004404"
     CDS             1248..1517
                     /locus_tag="RH058_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV3139873.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1594..2580
                     /gene="srkA"
                     /locus_tag="RH058_004405"
     CDS             1594..2580
                     /gene="srkA"
                     /locus_tag="RH058_004405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV3139874.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2597..3223
                     /gene="dsbA"
                     /locus_tag="RH058_004406"
     CDS             2597..3223
                     /gene="dsbA"
                     /locus_tag="RH058_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV3139875.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          816 a          776 c          785 g          852 t
ORIGIN      
        1 catccgtcag gatggccttt ttgcattggc gcagaaaaaa atgccggatg cgacgctggc
       61 gcgtcttatc cagcctactc ttgcttatcc gttctgcttt tgcatccact ccaccacaaa
      121 atcagccagc ccctcaacat cattaatatc cagtaatgcg acatcaagat taagcggcac
      181 atcactggct acagcaataa catgcctgtc tatcactaat tcttcaggtc gatgtccggc
      241 tccatcgcga aacagcacaa tctttgcgat ctcttcatgc ttaaaccctt cgaccagaat
      301 caaatccagc tttgaggtat ccattcgact tgcgagaaac tgtagatcca gctcttcttc
      361 gtctggtgtt tccgtcatca aggcccatcg ttgctggctg gcaacgatgg tttgtgccgc
      421 gccagccttg cgcagctcat agctatcttt gcctggctta tcaacatcca tatcatggtg
      481 cgtatgctta atcagccctg gacggatccc tctggcgcat aatgccggga tcagttgttt
      541 caaaagcgta gtttttccgg taccactcca cgcggcaaag gcgagtaacg gtatcatcgt
      601 ttttcctgcc atcgggcaag ctcctctggc gtattcacgt taataaatgc atctttatta
      661 tcgctgaaat caaccgcatg accgccagcc agacgcataa atgccattac ccggcgttct
      721 cctgcttgca gatattccag taataaaggc tcaattgcgc ggtttaccag agcaatagtc
      781 gggtgatcgc gttcaccgtc gtggacccac acgacaggcg catctttgcg ctgatgatta
      841 agccgggctg ctaaatcctc gggaatataa ggagtatcgc acggacaaaa caaaaaccac
      901 tcgcctgctt cctgctgcat tactgaaagc attcctgcca gagggcctgg gtaatccgcc
      961 agtgaatctt caatcacttt cagaccgctt gcctgataga tttcctgatg gcgattggca
     1021 ttaaccacga cgtgagagag ctgcgtcata agcgcgtcag cgacatgttg ccataatggt
     1081 ttgccgttta attcaagcaa tcctttatct acgccgccca ttcgtctggc tttaccgcct
     1141 gccagcacaa cgcctgttat cgtcgtcatc agattcaccg atatcgcctc ttttattgtg
     1201 ggattgaccc tgctaacgtg tctgtctcaa gagtaaggag cattactatg aaatgtaaac
     1261 gtctgaatga agttattgaa ctcctccagc cagcctggca aaaagagcca gaccttaacc
     1321 tgttgcaatt tttgcagaaa ctggcgaaag agtcaggttt tgacggcgaa ctggcggatt
     1381 tgacggatga cattctgatc tatcacctga aaatgcgtga ttccgcgaaa gatgcggtga
     1441 tcccgggttt gcagaaagat tatgaagaag acttcaaaac ggcgctgtta cgcgctcgtg
     1501 gcgtaattaa agagtaaaag cttgtaagcg gtgccaccaa aatcatcgtg aaatgatatc
     1561 cttcatcatt cgtaatgttt tccggatgat gggatgaaca acagcgcttt tactttccag
     1621 acactacacc cggataccat catggatgct ctatttgagc aagggatccg ggtggattcc
     1681 ggtcttaccc cgcttaacag ctatgaaaac cgtgtctatc aatttcagga cgaagatcgt
     1741 cgacgttttg tcgtcaaatt ttatcgccct gaacgttgga cagccgatca aatcctcgaa
     1801 gaacatcaat ttgcgttgca gttggtaaat gatgaagttc cggtcgcagc acctgtggcc
     1861 tttaacggtc agactttatt gaatcatcag ggattttatt tcgctgtttt tccaagcgtc
     1921 ggtggtcgcc agttcgaagc tgataatatc gatcagatgg aagcggttgg gcgttattta
     1981 gggcgtatgc accagacggg gcgcaaacag ctttttatcc atcgcccgac catcggtctg
     2041 aatgaatatc tcattgagcc acgcaagctg tttgaggacg ctacactgat gccttccggg
     2101 ttgaaagcgg cattcctgaa agcgacagat gagctgatcg ccgccgttac agcacactgg
     2161 cgggaagact tcaccgttct gcggctacat ggagactgcc acgccgggaa tattctctgg
     2221 cgcgatggtc caatgtttgt tgatctggat gatgcacgta atggtccagc tattcaggat
     2281 ttgtggatgt tgctcaatgg cgataaagcc gagcagcgga tgcaactgga aactattatt
     2341 gaagcttatg aagaatttag cgagttcgac accgctgaaa tcggactgat tgaaccttta
     2401 cgcgccatgc gtttggttta ttatcttgcc tggctaatgc ggcgttgggc tgatcccgcg
     2461 ttcccgaaaa atttcccgtg gttaaccggg gaagattact ggctgcgaca aacggcgact
     2521 tttatagaac aggcaaaagt tctacaagaa ccccctttac aattaacacc tatgtattaa
     2581 tcggagagag tagatcatga aaaagatttg gctggcgctg gctggtttag ttttagcgtt
     2641 tagcgcatcg gcggcgcagt atgaagatgg taaacagtac actaccctgg aaaaaccggt
     2701 agctggcgcg ccgcaagtgc tggagttttt ctctttcttc tgtccgcact gctatcagtt
     2761 tgaagaagtt ctgcatattt ctgataatgt gaagaaaaaa ctgccggaag gcgtgaagat
     2821 gactaaatac cacgtcaact tcatgggtgg tgacctgggc aaagatctga ctcaggcatg
     2881 ggctgtggcg atggcgctgg gcgtggaaga caaagtcact gttccgctgt ttgaaggcgt
     2941 acagaaaacc cagaccattc gttcagcatc tgatatccgc gatgtattta tcaacgcagg
     3001 tattaaaggt gaagagtacg acgcggcgtg gaacagcttc gtggtcaaat ctctggtcgc
     3061 tcagcaggaa aaagctgcag ctgacgtgca attgcgtggc gttccggcga tgtttgttaa
     3121 cggtaaatat cagctgaatc cgcagggtat ggatactagc aatatggatg tttttgttca
     3181 gcagtatgct gatacagtga aatatctgtc cgagaaaaaa taattcaat
//
LOCUS       DAPHDX010000242         3187 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPHDX010000242 DAPHDX010000000
VERSION     DAPHDX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.245"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            132..1814
                     /locus_tag="RH058_004407"
     CDS             132..1814
                     /locus_tag="RH058_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV3139876.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1866..3023
                     /locus_tag="RH058_004408"
     CDS             1866..3023
                     /locus_tag="RH058_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV3139877.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2989..3111)
                     /locus_tag="RH058_004409"
     CDS             complement(2989..3111)
                     /locus_tag="RH058_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139878.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          965 a          685 c          636 g          901 t
ORIGIN      
        1 ctttatggac tgttatttcc ctttttttag ctaaatctgc tatctcttta tgtgactaac
       61 ttcacttaca tccacttatt tctcttcgta aaattacttc ggaattaagt acaataagaa
      121 gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt aagtacctcg atatctttga
      181 tactggcatc tggtatggct gcatttgctg ctcatgcggc agatgatgta aagctgaaag
      241 caaccaaaac aaacgttgct ttctcagact ttacgccgac tgaatacagt accaaaggaa
      301 agccaaatat tatcgtacta accatggatg atcttggtta tggacaactt ccttttgata
      361 agggatcttt tgacccaaaa acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag
      421 ggatagataa agccattgaa gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg
      481 atgaaggcgt acggtttact aacggctatg tggcacacgg tgtttccggc ccctcccgtg
      541 ccgcaataat gaccggtcga gctcccgccc gctttggtgt ctattccaat acagatgctc
      601 aggatggtat tccgctaaca gaaactttct tgcctgaatt attccagaat catggttatt
      661 acactgcagc agtaggtaaa tggcacttgt caaaaatcag taatgtgccg gtaccggaag
      721 ataaacaaac gcgtgactat catgacaact tcaccacatt ttctgcggaa gaatggcaac
      781 ctcaaaaccg tggctttgat tactttatgg gattccacgc tgcaggaacg gcatattaca
      841 actccccttc actgttcaaa aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc
      901 agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa aacacttgac cagcctttta
      961 tgctttacct ggcttataat gctccgcacc tgccaaatga taatcctgca ccggatcaat
     1021 atcagaagca atttaatacc ggtagtcaaa cagcagataa ctactacgct tccgtttatt
     1081 ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa gaaaaacgga cagtatgaca
     1141 atacaattat tctctttacc tccgataatg gtgcggttat cgatggtcct ctgccgctga
     1201 acggggcgca aaaaggctat aagagtcaga cctatcctgg cggtactcac accccaatgt
     1261 ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg
     1321 atttctaccc gacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg
     1381 atggcgtttc cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc
     1441 tgacctggat aacctcttat tctcactggt ttgacgagga aaatattcca ttctgggata
     1501 attaccacaa atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg
     1561 acttaagcca attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag
     1621 aaaacaatca gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg
     1681 ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca
     1741 gccagccacc gcttagcgaa gtaaatcagg agaagtttaa caatatcaag aaagcactaa
     1801 gcgaagcgaa ataactaaac cttcatgcgg cggaattttc cgccgcctta ttgagcgaga
     1861 tagcgatgca cgttacagcc aagccatcca gttttcaatg taatctcaaa tgtgattact
     1921 gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc
     1981 tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa tcaggtctat tttacctggc
     2041 aaggcggtga acccactctg gctggcctgg attttttccg taaagttatt cactatcaac
     2101 aacgctatgc aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga
     2161 ataatgaatg gtgtgctttt ctcaaagaac atgagtttct ggttggtatc tcgatcgatg
     2221 gcccccagga gctacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa
     2281 aagtgatagc agccatcgag cgtctgaaat catatcaagt tgagtttaat acgttaaccg
     2341 tcattaataa cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg
     2401 gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg gacgccgaat attgatttca
     2461 gtggtcatag tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg
     2521 gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca
     2581 tccgtcagtt tgaaagcttt gtcagccgtt ttttagggaa tgggcatacc agttgtattt
     2641 tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagatatt tatgaatgcg
     2701 accattttgt ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga
     2761 tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt ctgaaatgtg
     2821 catataaacc tatctgcaat ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg
     2881 agactgtttc ttatttttgc gaaggttata aaatcctttt ttcaaccatg gtaccttata
     2941 tgaacgccat ggtggagtta gctaagaaca gagtaccgct ttaccacatt atggatgttg
     3001 caaaacaaat ggagaataat taataacgct ctcttttaac tccattctta gcctccttct
     3061 tatgacctta acaaggcacc cgacataaat tcctgtcggg tgtttcgtca tcatcatctt
     3121 cccttgcgaa aatcctggct tacgtggtat gcatcagggc attagcaata tccttgatta
     3181 tcaatag
//
LOCUS       DAPHDX010000243         3179 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPHDX010000243 DAPHDX010000000
VERSION     DAPHDX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.246"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..1638
                     /locus_tag="RH058_004410"
     CDS             43..1638
                     /locus_tag="RH058_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV3139879.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            1652..2803
                     /locus_tag="RH058_004411"
     CDS             1652..2803
                     /locus_tag="RH058_004411"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV3139880.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2846..>3179)
                     /locus_tag="RH058_004412"
     CDS             complement(2846..>3179)
                     /locus_tag="RH058_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3139881.1"
                     /translation="REKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEA
                     KWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEF
                     PQPVSLVG"
BASE COUNT          855 a          725 c          811 g          788 t
ORIGIN      
        1 catctggcgt agggaaaatc acatctgaat caggaattaa caatgaaacc tgtaaaacca
       61 cctcgtatta atggacgagt gccggtcctg tcggcacagg aagcggtgaa ttatattccc
      121 gacgaagcaa cactttgtgt gttaggcgct ggcggcggta ttctggaagc caccacgtta
      181 attacagctc ttgctgataa atataaacag actcaaacgc cacgtaattt atcgattatt
      241 agtccgacag ggcttggcga tcgcgccgac cgtggtatta gtcctctggc gcaagaaggt
      301 ctggtgaaat gggcattatg tggtcactgg ggacaatcgc cgcgtatttc tgaactcgca
      361 gaacaaaata aaattattgc ttataactat ccacaaggtg tacttacaca aaccttacgc
      421 gccgccgcag cccaccagcc tggtattatt agtgatattg gcatcgggac atttgtcgat
      481 ccacgccagc aaggcggcaa actgaatgaa gtcacgaaag aagacctgat taaactggtc
      541 gagtttgata acaaagaata tctctattac aaagcgattg cgccagatat cgccttcatt
      601 cgcgctacca cctgcgacag tgaaggctac gccacttttg aagatgaggt gatgtatctc
      661 gacgcattgg ttattgccca ggcggtgcac aataacggcg gtattgtgat gatgcaggtg
      721 cagaaaatgg ttaagaaagc cacgctgcat cctaaatctg tccgtattcc gggttatctg
      781 gtggatattg tggtggtcga tccggatcaa agccaactgt atggcggtgc gccggttaac
      841 cgttttattt ctggtgactt cacccttgat gacagcacca aacttagcct gcccctaaac
      901 caacgtaaat tagttgcgcg gcgtgcatta ttcgaaatgc gtaaaggcgc ggtggggaat
      961 gtcggcgtcg gtattgctga cggtattggc ctggtcgccc gggaagaagg ttgtgctgat
     1021 gactttattc tgacggtaga aacaggtccg attggcggaa ttacttcaca gggtatcgcc
     1081 tttggcgcga acgtgaatac ccgcgccatt ctggatatga cgtcccagtt tgatttttat
     1141 cacggtggtg gtctggatgt ttgttatttg agttttgctg aagtcgacca gcacggtaac
     1201 gtcggcgtgc ataaattcaa tggcaaaatc atgggtaccg gtggatttat tgatatcagt
     1261 gccacctcga agaaaattat tttctgcggc acgttaactg cgggcagttt aaaaacagaa
     1321 attgccgatg gcaaattgca tatcgtccag gaaggacggg taaataaatt tattcgggaa
     1381 ctaccggaaa ttacttttag cggaaaaatc gcgctcgaac ggggactgga tgttcgttac
     1441 atcaccgaac gtgctgtatt cacgctgaaa gaagacggcc tgcatttaat cgaaatcgcc
     1501 cctggcgtcg atttacaaaa agatattctc gacaaaatgg acttcacccc agtgatttcg
     1561 ccagaactca aactgatgga cgaaagatta tttatcgatg cggcgatggg ttttgtcctg
     1621 cctgaagcgg ctcattaaca ggagtattat gatggatttt tctttaactg aagaacaaga
     1681 actgctgctg gccagtattc gcgaactgat tacgactaac tttccggaag agtatttccg
     1741 cacctgcgat caaaacggga catatccgcg tgagtttatg cgggcgctgg cggataacgg
     1801 tatttccatg cttggcgtgc cggaagaatt tggtggtatc cctgcggatt acgtcaccca
     1861 aatgctggcg ctgatggaag tgtcaaaatg cggtgctccg gcatttttaa ttaccaacgg
     1921 tcaatgtatt cacagtatgc gccgtttcgg ttctgcagag cagctacgta aaacggcaga
     1981 gagcaccctg gaaacgggtg atcccgccta tgccctggcg ttgacggaac caggtgctgg
     2041 ctcagataac aacagtgcca ctaccactta cacgcgtaaa aacggcaagg tctacatcaa
     2101 cggacaaaaa acctttatta ccggtgcgaa agagtacccg tatatgctgg tgctggcgcg
     2161 cgatccgcaa ccgaaagatc ccaaaaaagc cttcaccctg tggtgggtcg actccagtaa
     2221 gcccggcatt aagattaacc cgctgcataa aatcggctgg catatgctca gcacctgcga
     2281 agtctatctc gacaatgtgg aagttgaaga gagcgacatg gtgggcgaag aaggaatggg
     2341 tttcctcaat gtgatgtaca actttgagat ggagcgcctg atcaacgccg cgcgcagcac
     2401 cggctttgcc gaatgcgcct ttgaagatgc cgcccgctat gccaaccaac gtatcgcttt
     2461 tggtaagccc attggtcata accagatgat ccaggaaaaa ctggcgctga tggcgattaa
     2521 gatcgacaac atgcgcaaca tggtgctgaa agtggcatgg caagccgatc agcatcagtc
     2581 actgcgcacc agcgcggcgc tggcaaaact gtattgcgca cgtaccgcaa tggaagtcat
     2641 tgatgatgcg attcaaatca tgggcggtct gggctatacc gatgaagcgc gcgtctcccg
     2701 cttctggcgt gatgtccgtt gtgaacgtat cggcggcggt acagacgaaa ttatgattta
     2761 cgtagcaggt cggcagatcc tgaaagacta tcagaacaaa taatctgcag gcggcgcagc
     2821 ttcttaacaa actgcgccgc cagatttatc caacaagact taccggttga ggaaattcag
     2881 ataggatttc ttgttccgca aaacagtttt taaattgtct acggtaatcg ctgggcgaac
     2941 aaccgacatg gcgcaaaaac agtttggcaa agtgatcgac attttcataa cccacacgcc
     3001 aggagatctc tgcctgtgat aattcagtat tagtgagtga ccattttgct tccgtcatac
     3061 gccgttgaat aacatagtta ataggggaaa tacgatactc tttggtaaat tcatggcaaa
     3121 tataactgac gctggcacga aattttttcg ataactgttc taaagtgatt ttttcgcga
//
LOCUS       DAPHDX010000244         3174 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPHDX010000244 DAPHDX010000000
VERSION     DAPHDX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.247"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1270..2754
                     /locus_tag="RH058_004413"
     CDS             1270..2754
                     /locus_tag="RH058_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV3139882.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            2970..3170
                     /locus_tag="RH058_004414"
     CDS             2970..3170
                     /locus_tag="RH058_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139883.1"
                     /translation="MQRVTERTESETAGRQAVQSALAPCPDTSFPETTGIRCAYRQPL
                     CGMPGARTDPSDPSVLRSASDG"
BASE COUNT          834 a          606 c          773 g          961 t
ORIGIN      
        1 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
       61 gacaaaaccc cgcgcaccgg aaaggtggcg ggggattcac ggagataata tctgtctgaa
      121 attgttttga atcgatggga ttgttgcgta ttaacatgaa ggagatcagg acttatccgt
      181 tactgggtga tctccttcca tgaaaatacg ccgttattgt taaacagcag gctgatttct
      241 ccgtaaaggg gaaaatattt atcggctgta tgattatcag gtgtgtctgt gtttactgct
      301 gtgagtattt ttcagtgtat tgtgggggag atgtcagtgg gagatattaa atttacattt
      361 ataattatct tacttcttct gtgtgttttt tataagagtg cacacgtaat gatatctgtt
      421 gataccatgt ggttaagtgt ttcccttcct ggtcatgtgt aatgattaaa tgcttttgtg
      481 acaccattct tccggtctga atatttatcc cgttcttttt tatttgtgca gtgatactgt
      541 cggaagagaa ttcagtaatg tgaggtgtcc tggtcaggat aatatctgct gagatattat
      601 tagttgagtg tgtttttact gagcaaaagc tggcttttaa ttcctgtact gaaacgctgt
      661 cgctcgtccg gggttaactg aaactggatg atgatgtaaa atgaaggctg tttttgtgtt
      721 tgtcgaagaa gtgttatttt aatccattct catcattggc tggcgattat acagaagtat
      781 ttgaaggaaa aatggtgcat tttatctgtg tcagcaggga agaagtattt tattgatgta
      841 tcagtattaa atggtttttg ttatttataa aaatgtcatc ctgtccatag aatattgtac
      901 tcagcattca gtgcaggaac tgtagtcttc tttattttat tcttcttcag gagggggggt
      961 gattcctgaa ggcgttattc ccggtgttct gtttatgatt cttcccggca ggaaaccggt
     1021 atctgcaata cccgggatga tgctttttgt tcgtacgtgg ttgtgtaaaa tttgaatagt
     1081 ggatgtgctc tgaagggaag gaggggacac acagattcat tttgtctttc ttcagcagca
     1141 aaacacactg tggttattat ttaaccatta aatcaacaaa aatttttacc tcatattgat
     1201 ttgactgcta tttttgctat taatagccag aattgcagtg cttttatatc tgtgtatagt
     1261 gaggttacta tgtcattcgg tcagccttgt gatgagtttc ccctgagttc gctgcctcca
     1321 ctcattcgtg atgccgttat tgaagctcag caaattacgc aggctcccct tgggcttgtg
     1381 gcagcgtctg ccctgggcgc tgtttcactt gtctgccaga atctgattga tgtctgcaga
     1441 ctgaatacac tgcgggggcc ggtctctctt tttttcctga cgcttgccga gtccggagaa
     1501 cgaaaaaccg cagtggacaa attattaatg aagcccctgt accagcagga gatgcagtta
     1561 tattccagat acaaaagtga actggctgtc tggaaaaaca aggaagagct tttaaaggct
     1621 cagaaaaaag cattgttgtc aaaactgaat aaagaactcc gcaaaggcgc ggatgagtca
     1681 gaaaccctca gacaactgga agtacttcag aaaaacagtg cggaagaacc tgtgaggtat
     1741 aaattcattt ttaatgatgc cacgactgcg gcaattaaga atcagctttg tggtaaatgg
     1801 cgttcagtgg gtattatgtc agatgaagcc ggtattatat ttgatggata taccctgtct
     1861 gagttgcctt ttattaacaa aatgtgggat ggctctgtat tgtctgtgga cagaaagaat
     1921 gaacccgaac agatgattga aaatgccaga atgacgttat ccctcatggt gcaacccggt
     1981 ctttttgacc gatatatgga gcgaaaaggt tctgttgcca gggacagtgg ttttcttgcc
     2041 cgttgtctta tcagtaaacc tgcaaccacg caggggaaac gctttattaa cggagctgtg
     2101 attccgggag gatcattaac ggcatttcat gaacgattaa tggaactggc cagaggcagt
     2161 attgaaaaat ccagtgagga tgaacggtat tgtctgcatt tttcgcctga agcgcaaaaa
     2221 atatttattg agcactataa tgttctggag caggatttat ccccttccgg acccttaagc
     2281 ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg caagaattgc agcgttattt
     2341 cagtatttca gctatggtga agggaaaatc tctgccgata tcatgacgtc tgctgtcgta
     2401 ataagttcgt ggtacacaga tgaatataaa aaattatttg cgttacctga tgaatctgaa
     2461 ctgcagcaga aggacgccga ggagctgttc gactggctca ttgaggagtg ccgcggagaa
     2521 tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg gttcagaaac
     2581 aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc agttcaggct ttctgtcgtg
     2641 ccggaaggaa aaaccatgta tgttctgctt ccccagatag ccagtttaaa gttgtctgat
     2701 gtctctggta tttttacctc gggttatcac tacaataagc tgagagcgaa gtaaccggtg
     2761 agcagttttt ctccattatg cttcagactt agatgattga ttattatggg gatttcatca
     2821 ccgatattca gatgtatgaa tatcggtgat gaactaaagc tcaggagctg cgtggtggtg
     2881 atgaatcaac aggcatataa ctgatacccg ccggtcagaa gttccagcct gcgactttcc
     2941 tgtctgcgtg cccgcagtcc ggcgttcagg tgcagcgtgt gacagaacgc acggagagcg
     3001 aaacggcagg cagacaggcc gttcagtcag ccctggcacc ctgcccggat accagcttcc
     3061 ctgaaacaac tggcatcagg tgcgcttatc gtcaaccgtt gtgcggtatg ccaggagcac
     3121 gtactgatcc atcagatccg tcagtgctgc gctctgcttc tgacggttga tgat
//
LOCUS       DAPHDX010000245         3142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPHDX010000245 DAPHDX010000000
VERSION     DAPHDX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.248"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(94..1284)
                     /locus_tag="RH058_004415"
     CDS             complement(94..1284)
                     /locus_tag="RH058_004415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3139884.1"
                     /translation="MAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRI
                     QTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGLLDRL
                     EGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISE
                     DGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYI
                     TPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITN
                     SAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQ
                     PAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKTKQGSSD
                     ASNY"
     gene            complement(1253..2377)
                     /locus_tag="RH058_004416"
     CDS             complement(1253..2377)
                     /locus_tag="RH058_004416"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017411.1,HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3139885.1"
                     /translation="MASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDE
                     RGRLTAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYT
                     TRYEYDRYGQQTAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTATVS
                     PDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLT
                     EQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDH
                     GWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTDGEDGPHPDGCYPDGRRADPPLPLR
                     QPAPPGVPHADTAWRATGREPLPLRPAGTANGETGMAAGA"
BASE COUNT          564 a         1062 c          786 g          730 t
ORIGIN      
        1 ccataaaata cgatgtcaga atttgaattg ttatcaaacc caaggtaaac aacaaataaa
       61 caaagggtaa ttaaaaaaat gacaagtttg cctctaataa tttgaagcat ctgaactccc
      121 ttgctttgtt tttattggat tatctgcttt tataccatct aacccgaaga gttgcccagt
      181 gttatatttt tcaatacatg catcgctaca tttggcagaa aaatcgccac aagcaatacc
      241 gacagtgtta tttttaagat ccatgattct ttctttagct ggctgacctt gtctatcccc
      301 tgccgcctca tgatttttac ctatcacgtc agctattgtt gatccaaatg tcttagtcat
      361 tcgacacatc aagtaacaat gtctcattgc atcggcggcc ccattatgag ctccgccaac
      421 aatagcatcc atatcataac cgaactggta agcactgtta gtaatgctgt ttgcagcaat
      481 caatccaggt acaccagata cggcataaat tagtccacct gatgtcatat tctcaatcgc
      541 atctaacccc attgggtcta tcacttgtat gggattcaac ggatactgat acatattcca
      601 tccacctctc aacccaatcg ggtccggggt gatataccgc ccctgcaacg gatcgtagta
      661 ccggtgacgg ttatagtaca gccccgactc cttatcatac tgctgccccg gcagccggta
      721 cggctggtgc aggtgatgcg ggttctcctc attaagctgg ttgccccatt catcatattc
      781 cgcgctccac gccgtattgc cgtcttcact gataagcgcc agcggcaggc cccggtggtc
      841 acagtgataa agatgtattt ttcgctcagg caaataaacc ggctctatct gtgccgccag
      901 ccgctccacc gtcagcccgc actgcgcaag ccactggcgg ctttcactgc tcacgcagtt
      961 tgcccggatt tccccctcca gcctgtccag cagccccacc agttcagccg gaaataccac
     1021 gccgtgcccg tcctcgctcc cttcctgctg tatcttctcc gccaggctgc ggcactgcgc
     1081 tttctcccgt tcaccgttgt ctgtttcgat gcggatgagc ggcgcgaagc tccccggctg
     1141 gtataccgtc tggatacgcg tggtgtcggt ctgtaccgtc gtcagcctgt cgccgtccca
     1201 gccgtaccat gtctcctccg gtttacgcga cagcgacatc caccccgtca ggtcacgctc
     1261 ccgccgccat acccgtttcg ccattcgccg tcccagcggg tcgtagaggt agcggctctc
     1321 gaccagtggc tcgccatgct gtatccgcgt gtggaacacc aggcggtgct ggctgtcgta
     1381 gtggtagtgg tgggtccgct cgtcgtccgt ccggataaca cccgtcggga tgcggtccgt
     1441 cttctccgtc agtctgccgt tcgcccgtca gacggccttt atcgtcatag ccgtagtgga
     1501 ccgccacacg gtggccttca ctcaggtggc tgatttctgt cagccagccg tggtcgtcat
     1561 actgccactg ctccgccggt tcaccgttca ccgtgcgccg ggtgatacgg tccgacgcat
     1621 cgtaatgcca gagggtgata agcccctcat cttcactctg cgtgagtttt ccggtcaggt
     1681 catactggta acgttgcgtc cggccgtcaa agccgcgctg ctccgtcagc ctgtcgaccg
     1741 ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacagtg atgcgcccgg
     1801 cagcgtcata ccccatgctg cgcgtcagac cgccttccgt cacactcacc ggacgcccgc
     1861 gtttatcata ttcgatggtg ctgcgtttac cgtccggtga taccgttgct gtcaggtcgc
     1921 ctgcggcgct gtactcataa cgggtctccc gcccctgcgc atccctctga ctgaccagtt
     1981 gaccgcgcgg gttataactg ctgtaagtgc tgatgccttc ttcccggtga acggcggttt
     2041 gttgaccgta ccggtcgtac tcataccgcg tcgtgtaccc cgagcagtcc gtgaaggcca
     2101 gcagttgacc gtaacggctc catgccatct gttttgtact gcccgtcgcg tcctgtatcc
     2161 ctgtcggcag ctcactcgcc gggtcatcat agctgtaccg cgttgtctca ccgctgcgcg
     2221 aggtctccgc cgtcagcctt cccctctcat catactcccg gctgctgcga agcccgtccg
     2281 ggtacgtcac tgacgtcacc tgccgctggc tgttatagcc ataccgcacc gtcctgccgt
     2341 ccggccccgt caccgctgtc accgcgcccg acgccatatg cagccggtac tccgtccgcc
     2401 gccccgccgc atccgtctgc gccttcagcc tccccgcctc atcatactcg ctgcgggtga
     2461 tgctcccgtc ggcatgctcc ttcttcacca cccgcttcag gccaccctcg ccttccgtgt
     2521 acagcacctc ccgccggttc aggctgtccg tgatgattac ccggctctct ccgtactcaa
     2581 agcggtagtc cagcccctcc gggttgacct gctccgtcac ccgaccggta tcatcatacc
     2641 ggtagcagct ctccggcctc cccgcataat ggtgcgccac catccgcccg gcgtgctccg
     2701 catcataagt aaacccgcgc acctgcatcc cgctgcggtc atacaccgcc cgcagttcac
     2761 caccggccgt gtacgtgtag cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg
     2821 ggtcgtgtgt cagccatacc gcctccagcc ggataccgtt atcggcaccg tactctgtac
     2881 cggcgggcag cgtgtcgggg aaaacctgag aagaggaaac agaacgggga ctggccgggg
     2941 aagataagga agaggcgcgc tgtttacgga aggcttccgc ccgctgcgcc tgcgtggtca
     3001 gcgccaggtg aaaacggcgg cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt
     3061 cacccttagc tgcccggtga aaggccagcg tccgcccgaa gccgtccacc acccccgtca
     3121 gcacccggta tgcgggcggc gg
//
LOCUS       DAPHDX010000246         3133 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPHDX010000246 DAPHDX010000000
VERSION     DAPHDX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.249"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..459
                     /locus_tag="RH058_004417"
     CDS             31..459
                     /locus_tag="RH058_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139886.1"
                     /translation="MTNKKTQFLNTKDAKHSTSKCNLLPQELRIHCVSVRLNNTELAK
                     LNTLREHYSKGEWLRMASLQKLPPVIPAINTKAWIALTDISQKLNRIAIHIDGKSKDS
                     KLTHTELFAVKRQLEELRQHLLNADIWSKPYEGYAEDQKG"
     gene            428..1588
                     /locus_tag="RH058_004418"
     CDS             428..1588
                     /locus_tag="RH058_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDV3139887.1"
                     /translation="MKGMQKIKRGKSFAGVVQYALKPGSHHKSNPIVIGGNMLGGSAL
                     ELISEFNGTKQLRPDVQKAVWHNSLRLPEGETLSNDQWVSIADDYMKKMGFSDTHLRC
                     YVLHDDEGQHIHIIASRIDIAGGKLYLGRNENLISTRIISELEMSHGLTVTKTASPPS
                     QAQPKRKRVSRNEQMLSARTGVPCPKEALQQIIDKSMTDKPDLLTFVKRLEDSEVGCK
                     ANIASTGKMNGFSFEYRNIAFKASQLGKAYSWVNLQKQLNYNQNHLEVLRATKEAIPI
                     PVPVPVPVPVPVPIESTARTTAQSESIGEKIAEIEQRLREDRRNEIVEKILQKNAVKQ
                     QKHLRLIGWLPFLRRLAELLRSYGKSILHKPLPGFSKLDATRTLHKGRKIHL"
     gene            2126..2512
                     /locus_tag="RH058_004419"
     CDS             2126..2512
                     /locus_tag="RH058_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139888.1"
                     /translation="MNRFIKSSLFIIVFLLSGCSTSGNQNLKNETPQSLQSKIIKNKT
                     TKTELLTKLGEPDTRTTLDDGNEQWRYFMYNNQFNATTFIPVVGLFTGGSQTQSKTIE
                     IDFKGEIVNKWTFSTDNNNTKTGVIN"
     gene            2531..2845
                     /locus_tag="RH058_004420"
     CDS             2531..2845
                     /locus_tag="RH058_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HDV3139889.1"
                     /translation="MKIYSILTVILTTCLLTACNSEPSQDDIYNVFKNVVDRSNASMK
                     ALNSSIPEKDLLRIDYVKKVSCTEEANNVYNCIVDASISNMKQTKPVKLVKADGVWKE
                     VQ"
     gene            complement(2897..>3133)
                     /locus_tag="RH058_004421"
     CDS             complement(2897..>3133)
                     /locus_tag="RH058_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139890.1"
                     /translation="IDNIMAMFSSVYKSLTSNLLQRLNNKQGVLRELNSLVNYIDNNQ
                     EKAEEIYATVRAQYEMKVIEKELIHEVRVRTIRL"
BASE COUNT         1012 a          654 c          611 g          856 t
ORIGIN      
        1 tttaaagcat gacatataaa aggagtttat atgacaaata aaaaaacgca gtttttgaac
       61 actaaggatg caaaacattc aacctcaaag tgtaatttac taccacagga attacgaatt
      121 cattgtgtta gtgttcgtct taacaatact gaattggcca agcttaatac attgagggag
      181 cattattcga aaggagagtg gcttcgtatg gcttcccttc aaaaattgcc tccagttata
      241 ccagctatta acaccaaagc ctggatagct ttaactgaca tatcgcaaaa gctaaatcga
      301 attgcgatcc atatcgatgg taagagcaaa gacagcaagc ttacgcatac agagcttttc
      361 gcagtgaaac gacaactgga agagcttcgc cagcacctgc taaatgctga catatggagc
      421 aaaccctatg aagggtatgc agaagatcaa aaggggtaaa agcttcgctg gtgtcgtgca
      481 atatgcattg aagccaggtt cacatcataa aagtaatcca atcgtcattg gcgggaacat
      541 gttaggtggc tctgctcttg aattgatttc cgaattcaat ggcacaaaac aacttcgccc
      601 agatgttcag aaggccgttt ggcacaactc gttacgattg cctgagggag aaacattatc
      661 gaatgaccaa tgggtgtcca tagctgacga ctacatgaag aagatggggt tcagtgatac
      721 tcacctccgt tgctatgtct tacatgacga cgaaggccag cacatacaca ttatcgccag
      781 cagaatcgac atagccggtg ggaagctcta cttgggtagg aacgaaaacc ttataagcac
      841 acggatcatt agtgaactcg aaatgtctca cggtctgaca gtgaccaaaa ctgcatctcc
      901 cccctcgcaa gcacaaccga agcggaagag agtgtcccgt aacgaacaaa tgctatcggc
      961 ccggactgga gttccctgcc ctaaggaagc tctccaacag atcattgata aaagtatgac
     1021 tgataagccc gaccttctta cttttgttaa acgactcgaa gattcagaag tcggctgtaa
     1081 ggccaatatt gcttctaccg gtaagatgaa cggcttctct ttcgaatacc gcaatatagc
     1141 tttcaaggca tctcaacttg gaaaggcata ctcatgggta aatcttcaaa agcagcttaa
     1201 ctacaaccaa aaccacttag aagttcttcg agcgacaaag gaagctatcc ccatccctgt
     1261 tcctgttcct gttcctgttc ctgttcctgt tcctatagaa tcgacagcaa gaacgactgc
     1321 ccagagcgaa agcataggcg aaaaaattgc tgaaatagaa cagcgactca gagaagacag
     1381 acgaaacgag atcgttgaga agattcttca aaagaatgca gttaaacagc aaaagcatct
     1441 aaggcttatc ggctggcttc catttcttag aaggcttgca gagcttctcc ggagctatgg
     1501 taagagcatt cttcacaagc ccctccctgg attctcaaaa ctcgatgcca cacggactct
     1561 acacaaagga agaaaaattc atttatagac aaaaatttac cgcctatccc tgacagcaat
     1621 atcattttat tagtagtaaa tttaggataa gtcaacaata aattgatcgt tattacagat
     1681 cgataattct attatgatag atatcgacta tcaagcaacg atcatcgatc ggtacaagtg
     1741 attttacttt agccagccag ccatcgagaa tccctacaaa cacgcagtaa atcttccacc
     1801 ctggggttgc tatgaaaaac gaaacaatta agtgcccgtt ctgctttaca caaagtcctc
     1861 atggtgtgcg ggtttgtaaa ggttgccatg caaagatcgc ttacggtgaa agcccgctca
     1921 gtgtggcatt cctgtttcaa ttcgttgcat ttgggctcgc gtggttagca ttttcgtgga
     1981 ctggcagcac tcttgtatca gtcattacgt ttttcgtaag catcattatt cttatctata
     2041 aaattaaaaa aacgtatgca gacagagttg tttttatacg gtgaaattaa tttcacacaa
     2101 ccgcaaacct aaaatgagaa agtaaatgaa ccgatttata aagtcctctt tatttattat
     2161 tgtcttcctg ctttctgggt gttcaacatc agggaaccag aaccttaaaa acgaaacacc
     2221 tcaaagcctc cagtcaaaga tcattaaaaa caaaacaact aagacggaat tactcactaa
     2281 attaggtgag cctgatacaa gaaccacact cgatgacgga aatgagcagt ggagatactt
     2341 tatgtataac aatcagttca atgcaacaac tttcattcca gttgtagggc tgtttactgg
     2401 cggttcgcag actcagtcta aaactattga aatagatttc aaaggcgaaa ttgtcaacaa
     2461 atggacgttc tccactgaca ataacaacac caaaacgggc gttattaatt aattattttt
     2521 ggataataaa atgaaaatat actcaatcct tacagttatc cttactacat gtcttctaac
     2581 tgcatgtaac agtgaaccat cgcaagacga tatatacaat gtttttaaaa atgttgtaga
     2641 caggtcaaat gcaagtatga aagctctgaa ctcaagtatt cccgaaaaag atcttttaag
     2701 aatcgattat gtgaagaaag taagttgcac agaagaagca aacaatgttt ataactgcat
     2761 tgtcgatgcc tcgattagca atatgaaaca gacaaagcct gttaagcttg tgaaagctga
     2821 tggtgtctgg aaagaagttc agtagtagta atatcgagaa gtctctttta gcccctgcat
     2881 aggggctttt tattgcctat aatctgatgg ttctcacgcg aacttcatga attaactctt
     2941 tctcaattac tttcatttca tattgtgctc tgacagtagc atagatttct tctgcttttt
     3001 cctgattgtt atctatgtaa ttaacaagcg agtttagctc tctaagaaca ccttgtttgt
     3061 tatttagtcg ttgtagtaga ttgcttgtca gacttttgta taccgagcta aacatagcca
     3121 ttatgttatc aat
//
LOCUS       DAPHDX010000247         3110 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPHDX010000247 DAPHDX010000000
VERSION     DAPHDX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.250"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1826)
                     /locus_tag="RH058_004422"
     CDS             complement(<1..1826)
                     /locus_tag="RH058_004422"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015188.1,HMM:NF027494.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3139891.1"
                     /translation="MNRFSQIVWNESTGEVGSDLSTGQEGQQQVKIRQTVLAPLIASF
                     LLTPSSVLAENYSNETLPNSTTAGSVLNEGDIATNTTINSGGHQYVYSGGSATDTLIS
                     GGDQHVSSGGSAINTIINGLHYKDGSQYVSSGGNAINTIINDEGEQYVYNGGNAISTT
                     INSRGVQVVSNGGIATNTTINGGGQIVYSGGKVSVSRLNSGHQYVYSGGSATDIIIDR
                     FGNQIVYSGGRVSNTTINGGEQLVIGGHAISTTINSGGKQTVHTSGSVTSTTINNGGV
                     LWVGSYSTGTDIVQNDGGAIITDTDETVSGFNTNGEFSIADGRASNMLLENGGSLTVL
                     PGHSVKDTMVGSGGQLGVVKDILLLGNNTIADSGKIVSDEITLSITNNGHLHFLNNSA
                     ATYTFDLTGTGDLKVDGGHLVLSGTLSQDGGVSIVNSGTMVMDNLQATADLTMQADTS
                     LSLEQNSTLAGRIVGDGAGAGNVTVRSSLWELRGDSTVGALTMDNGTVHFHSPQSENW
                     QPAILTAGSLSGNGTFRMNTDIAAHTGDLLNVNGNASGNFVLNIRNTGQEPASAGSPL
                     RVVHTGSGDARFTLDGGKVDAGTWEYYLNKENTDWYLKADVT"
     gene            complement(1819..3030)
                     /locus_tag="RH058_004423"
     CDS             complement(1819..3030)
                     /locus_tag="RH058_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HDV3139892.1"
                     /translation="MKTSFIYPPEIPTQSGPKHICYDFNDGARVLLPDGKWHVRLIDD
                     DSGNILFQCDSGKGWVTSTKKYFVRFRIQVYQQGSTHPILDETLNLTDKPVLISFPTG
                     TLGDLLGWFPYAERFQKQHQCRLECAMAQNIIDLLASQYPQIHFSTPDKLQTVLPYAT
                     YRVGLYFGGDTDNQPVDFRQVGFHRNAGYILGVDPREEPVRLDLSSPRTIQEPYVCIA
                     TQSTCQAKYWNNGTGWSEVVAHLKSLGYRVLCIDQYAHHGEGLIWNHIPWGTEDFTGS
                     HPLQERVNLLRHASFFIGLSSGLSWLAWATRIPVVLISGFSLPGSEFYTPWRVFNSHG
                     CNGCWDDTSLNFDHKDFLWCPRHKDTPRQYECTRLITGRQVNGVIDRLHLALAEQNGS
                     VTRDKKGVTDE"
BASE COUNT          820 a          799 c          653 g          838 t
ORIGIN      
        1 tcagtgacat cagctttcag ataccagtcg gtattttctt tattcagata gtattcccat
       61 gtacctgcat caacctttcc accgtccagt gtaaatctgg catcaccact gcctgtatga
      121 acaactcgca gcggcgagcc tgcagaagct ggttcctgtc cggtatttct gatattcagc
      181 acaaagttac cgctggcatt tccatttaca ttcaacaaat ctccggtgtg agcagcaata
      241 tccgtattca tcctgaaagt tccgttccca gacagacttc cagccgtcag aattgcaggc
      301 tgccagtttt ctgactgcgg agaatgaaaa tgaacagtgc cattatccat tgtaagtgcg
      361 ccaacagtgg agtctcccct cagttcccat aaggaactcc gcacagtcac atttcctgca
      421 ccagcgccat ctcccacaat acgtcctgcc aatgtgctgt tctgctccag actcaggctt
      481 gtgtcggcct gcatcgtaag atctgctgtt gcctgcagat tatccataac catagtgcca
      541 ctgttcacga tggagacccc accatcctgt gacagagtac cactgagcac caggtgaccc
      601 ccatccactt tcaggtcccc agttcctgtc agatcaaaag tataagttgc tgcactgttg
      661 ttaaggaaat gcaaatgtcc gttgtttgta attgaaagag ttatctcatc acttacaatc
      721 ttaccactgt ctgctatcgt gttattgcca agcagcaaga tgtccttaac gaccccgagt
      781 tgaccaccac tgcctaccat ggtatcttta acagaatgtc ctggaaggac agtcagacta
      841 ccaccgttct ccaacagcat attgctggca cggccatcag cgatactgaa ttcgccatta
      901 gtattgaagc cgcttacggt ttcatccgta tctgtgataa tagcccctcc atcgttctga
      961 actatgtctg ttccagtgga ataactcccg acccacaaaa ccccaccatt gttaatggtg
     1021 gtagatgtca cactgccgct ggtatgaaca gtttgtttac caccactatt aatggtggta
     1081 gagatggcat ggcccccaat gacaagttgc tcccctccgt taattgtcgt gtttgagacg
     1141 cgaccaccag agtaaacgat ctggtttcca aaacggtcaa tgataatgtc tgtggcactg
     1201 cctccactat agacatactg gtgcccactg ttcagtctgg aaactgagac tttgccaccg
     1261 ctgtaaacaa tctgcccacc accgttaatg gtagtgtttg tggcaatgcc tccgttggaa
     1321 acaacttgga ctccgcgact attaatagtg gtagatattg cattgccacc attgtagaca
     1381 tactgctccc cttcatcgtt aattatagtg tttatagcat taccaccgct ggagacatac
     1441 tgactaccat ccttgtaatg gagaccattg atgatagtat tgatggcgct gccgccgctg
     1501 gagacatgct ggtctccgcc actaattaat gtgtctgtag cactgcctcc actatagaca
     1561 tactggtgcc caccgctgtt aatggttgta ttcgttgcta tatcaccttc attcaatact
     1621 gagccagcag tagtgctgtt gggcagagtt tcattgctgt agttttctgc cagaacagat
     1681 gatggcgtca acaggaaact ggcaatcaat ggcgccagta cagtttgtct gatttttact
     1741 tgttgttgac cttcctgacc agtgctgagg tcactaccca cttcccctgt ggattcattc
     1801 catactatct gggaaaacct attcatcagt tactcctttt ttatcacgtg tcaccgaacc
     1861 attctgttct gcaagtgcaa gatgaagtct gtcgatcaca ccattaacct gcctgccagt
     1921 tatcagtcgg gtgcattcat actgtcgtgg ggtatcttta tgcctcggac accagagaaa
     1981 atccttatgg tcaaaattca gtgatgtatc atcccagcaa ccgttacaac catgggaatt
     2041 aaacacacgc cacggggtat agaactcgct ccccggaaga ctgaaaccac ttatcagtac
     2101 caccggaata cgggttgccc acgccagcca tgacagacca ctggaaagac caataaaaaa
     2161 actggcatgt cgaagcagat taacccgctc ctgcaaaggg tgactgccag taaaatcctc
     2221 agtaccccac ggaatatggt tccatatcaa accttctccg tgatgagcat actggtcaat
     2281 acacaatacc cggtaaccca gagatttcag atgtgccacc acctcactcc agcctgtacc
     2341 gttgttccag tatttcgcct gacaggtgga ctgggtagca atacacacat aaggctcctg
     2401 aatagttctt ggtgatgaca agtcaagacg taccggttct tcacgaggat caaccccaag
     2461 aatgtagcca gcattacggt gaaaacctac ctgacggaaa tccactggct ggttgtctgt
     2521 gtctccgccg aaatacagcc caactcggta agtagcataa ggtaaaactg tctgtagttt
     2581 atctggcgtg gaaaaatgta tctgaggata ttgagatgcc aacaggtcga taatattctg
     2641 tgccatcgcg cattctaacc ggcattgatg ctgtttctga aaacgttcgg cgtaagggaa
     2701 ccaaccgagt aaatctccaa gagtaccagt gggaaatgaa atcagcacgg gcttatctgt
     2761 caaattcagg gtctcgtcca gtattggatg tgttgagcct tgttgatata cctgaatacg
     2821 gaaacgcaca aaatatttct ttgtgctggt gacccaccct tttccagaat cacactgaaa
     2881 aagaatgtta ccagaatcat catctatcag acgtacatgc catttaccat caggcagaag
     2941 cacgcgggct ccatcattaa agtcataaca aatatgctta ggtccgctct gggttggaat
     3001 ttctggtggg taaataaaag atgttttcat tttgtatctg gataactcgc agtaattaat
     3061 ggatgcgggc tcattaaaca taatgaattc aattgctgaa ctggctgcca
//
LOCUS       DAPHDX010000248         3048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPHDX010000248 DAPHDX010000000
VERSION     DAPHDX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.251"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1083
                     /locus_tag="RH058_004424"
     CDS             <1..1083
                     /locus_tag="RH058_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV3139893.1"
                     /translation="TYMRQYSIASLIFISLSVNAAHTGATFSQKGGEMSNQASPDGWT
                     ISQEGRVGAGNATDKRISIGTESEYTNKSVGYDPFTGKDIDGSQNATISIGNSNYKRQ
                     IKNVENGIDDYDAVNVSQLKAVSNSASEAISIVNNANTSINQAVNIANSLNDTVSKGI
                     NIGADNGPVVKRELGDTVTVTGDSNIRTKTTSTGVKVALNQNINLGENGSVNTGNTTV
                     NSHGVIINEGPSMTRSGFNAANNRITNVAPGINNTDAVNVGQLNNVVSNVGKSIQNLN
                     NRINHVEKNANAGIAEAMATAGLPQAYLPGKSMMAIGGGSYSGETGYAIGYSTISDNG
                     RWMFKGTGSGNSRGNYGGTLGVGYQW"
     gene            1142..1981
                     /locus_tag="RH058_004425"
     CDS             1142..1981
                     /locus_tag="RH058_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3139894.1"
                     /translation="MTFIMRKKKVSLILGILLLAGCGSGTVIKNNGTTDAPKWHKWNK
                     VILNNHLGTFPNLQSLNQVREGMTKDQLYYLIGRPQYDDGWRPREWNYLFHFYTPGKG
                     VNNVTTCQYKILFDSKMIARSFYWHPVIPEDSVCPPQKSSASQKFTQRYTLNADALFP
                     FDKSDASDINQQGIVSLDNLAMKLNSFEQLNSIRVIGYTDRLGSPDYNSILSKKRADT
                     IRQYLIAKGIPAGKIIAIGMGKNQPVKECDYENNRTALINCLQPNRRVEVEVDGIIRI
                     SDE"
     gene            1978..2977
                     /locus_tag="RH058_004426"
                     /pseudo
     CDS             1978..2977
                     /locus_tag="RH058_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          978 a          525 c          723 g          822 t
ORIGIN      
        1 acatatatga gacaatattc aattgcttct ttaatcttca tttcattatc tgtaaatgct
       61 gcacatactg gagcaacctt ttctcaaaaa ggaggagaaa tgtcaaatca ggcctcgccg
      121 gatggctgga ctatatctca ggaaggacgt gttggagcag gtaatgctac agataaaaga
      181 atttccattg gtacagaaag tgaatatact aataaaagtg tagggtatga ccctttcaca
      241 ggcaaagata tcgatggtag ccagaatgca acaataagta ttggtaatag taattacaaa
      301 cggcagataa aaaatgtaga gaatggtatt gatgattatg atgctgttaa cgtttctcag
      361 ttaaaggctg tcagtaatag tgcatctgag gctattagca ttgtcaataa tgctaatact
      421 agtataaacc aggcggttaa tatagctaac agtttaaacg atacggtcag taaaggaata
      481 aatataggtg ctgataatgg tcctgtagta aaacgagaat taggagatac agttactgta
      541 accggagaca gtaatataag aaccaaaacc accagtaccg gggtcaaagt cgctttaaat
      601 cagaatatta atcttggtga gaatggtagc gtgaatactg gtaacacaac cgttaattct
      661 catggtgtaa ttattaatga aggcccgagc atgacgcgga gtggctttaa cgctgctaat
      721 aatagaatca ccaatgtagc tcctgggata aataatacag atgcagttaa tgtgggacag
      781 ctaaataatg tagtatctaa tgtagggaag agtattcaaa atttgaataa ccgcatcaac
      841 catgtggaga aaaatgcaaa tgcaggcata gctgaggcta tggccacagc cggactgccg
      901 caggcttatc tgcctggtaa gagtatgatg gcgataggtg gcggttctta tagtggtgaa
      961 acaggctatg caattggcta ttccaccata tcggataatg gcagatggat gtttaaaggt
     1021 actggcagtg gtaattcacg tggtaactat ggtggaaccc ttggtgttgg ttatcagtgg
     1081 taatactata ttcttggtgt acatttttat aaaaaacttc ctatatatgt aaggaattac
     1141 aatgactttt attatgagaa aaaagaaagt atcattaata cttggtatac tactattggc
     1201 tggttgtggt tcagggactg ttattaagaa taatggtaca accgatgctc ccaaatggca
     1261 taaatggaat aaagtgattt tgaacaatca ccttggaact tttcctaacc tgcaaagcct
     1321 aaatcaggta cgtgagggta tgaccaaaga ccagttatat tacttgattg gtcgaccgca
     1381 atacgatgat ggatggcgac cacgggaatg gaactatctg ttccattttt atactcccgg
     1441 taaaggtgtt aataatgtca caacatgtca atataaaatc ttatttgaca gtaagatgat
     1501 agcccgtagt ttttactggc atccggtaat tccggaagat agtgtgtgtc cacctcagaa
     1561 atcatcggca tcacaaaaat ttacacagcg atatacgctg aatgccgatg cacttttccc
     1621 atttgataaa agtgatgcgt ctgatatcaa tcaacaggga atcgttagcc tggataatct
     1681 ggctatgaaa ctgaattctt ttgagcaact caatagcatc agggttattg gctatacgga
     1741 caggttgggg agtcctgatt ataatagtat tttatcgaag aaacgtgctg atacaattcg
     1801 tcagtacctg attgctaaag gaatacctgc aggcaagatt attgctattg gtatggggaa
     1861 aaaccagcct gtaaaagaat gcgattatga aaataacagg actgcactta tcaactgctt
     1921 acaaccaaac cggcgtgtgg aggtagaggt ggatggtatc attagaataa gtgatgaata
     1981 agtagaactc ctcaggaaga aatcgtcatt attcagcaga caatagagcc aggagtgact
     2041 gtgtccctta tggcgcgttt gcatggtatt cagcccagcc agcctgttgt ttaaatgaaa
     2101 aacaatataa ggaaggaggt ctccctgctg ttgctgccgg agaacaggtt gttcctgctg
     2161 ccgctataaa ataaataaaa gagttacagc acttgctggg taagacaacc atggaggttt
     2221 atatcatcaa cgaagctgtg gagtataatt actcgtgaaa atcgatagag catgcgcctt
     2281 ggtgcccaac ggataaggaa caactactgt aagctgtatt ctgggcgtgt cgcgtgctca
     2341 actgacacaa cggatgaaaa catctggtaa taagctggat aaacgtcgtc gcagacgcga
     2401 tgatgctgcg gatgcggaag tgttagcacg gatatgccga catatggtta tcgtggtgtc
     2461 tgtgccattc tcagataaca gtcccgtaac gaaggactgc tgtttgtgaa tgcaaagcgg
     2521 gtttatcgga taatgaccga aaataacctg ttgttacatg acgaaccttc attgccacag
     2581 cggaaacata taaaggtcga atatcggtga aggaaagcga ccagctctgg tgttacgatg
     2641 gctttgagtt aggttgcgat gatggcgaaa aactgcgtgt cacgttcgca cttaactgtt
     2701 gcgatcgtga agccataaac tgggccgcaa gtacgtgagg ttatgacaaa aaacagtgca
     2761 ggatgtcatg ttaggagcga tagataacgg aatggcagaa aggtttttga aaacgatgaa
     2821 ggaagactat atcgttttca tgcacaaacc aaatgtaaga ccagctttgc ataaccttgt
     2881 agtggcgatc gagcattaca atgaaaacaa tccgcatagt gcgttaggtt atcgctcgcc
     2941 acggaaatat cgacgtcagc gggtaacgtt aacttaagat acaacaactg tctgaatata
     3001 gtggactaag gacatcctgt tgtcaggata tgggtttttg ttaattat
//
LOCUS       DAPHDX010000249         2985 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPHDX010000249 DAPHDX010000000
VERSION     DAPHDX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.252"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..190)
                     /locus_tag="RH058_004427"
     tRNA            complement(114..190)
                     /locus_tag="RH058_004427"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Asp,seq:gtc)
     gene            complement(323..1054)
                     /gene="dnaQ"
                     /locus_tag="RH058_004428"
     CDS             complement(323..1054)
                     /gene="dnaQ"
                     /locus_tag="RH058_004428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV3139895.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1119..1586
                     /gene="rnhA"
                     /locus_tag="RH058_004429"
     CDS             1119..1586
                     /gene="rnhA"
                     /locus_tag="RH058_004429"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV3139896.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1583..2305)
                     /locus_tag="RH058_004430"
     CDS             complement(1583..2305)
                     /locus_tag="RH058_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV3139897.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2339..>2985
                     /gene="gloB"
                     /locus_tag="RH058_004431"
     CDS             2339..>2985
                     /gene="gloB"
                     /locus_tag="RH058_004431"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV3139898.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLKAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPPETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINV"
BASE COUNT          807 a          723 c          704 g          751 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 accgcaaaat taaaaatctt gcggttctct gaactcattt tcatgagtga atttggcgga
      121 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
      181 ctaccgctcc gcgttgtgtt ccgttgggaa cgaggcgaat attacggatt gcctcaccct
      241 tcgtcaacgg tttttctcat cttttgaatc gtttgctgca aaaatcgccc aagccgctat
      301 ttttagcgcc ttttacaggt atttatgccc gccagaggca gcttccgccc ttcttctcca
      361 ccagatcaag acgggcttca tgagctgcaa gctcttcatc tgtcgcaaaa acaacgcgta
      421 acttacttgc ctgacgtaca atgcgctgaa ttgttgcttc accttgttgc tgctgtgtct
      481 ctccttccat cgcaaaagcc atcgacgttt gaccaccggt catcgccaga taaacttccg
      541 caaggatctg tgcatcgagt aatgccccgt gtagcgttcg tttactgtta tctatttcgt
      601 agcgggcaca taacgcatcg aggctgttgc gcttaccagg aaacattttc ctcgccaccg
      661 caaggctatc ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg
      721 aaaactcgta gtccataaag ccgatatcga acgctgcgtt atggatcacc aactccgcgc
      781 cgcggatata gtccatgaac tcatcggcta cctcggcaaa cgtgggctta tcgagcaaaa
      841 attcatcggc aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga
      901 gataaacatg gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct
      961 caatgatctt gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat
     1021 cgagaacgat ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttgtcg
     1081 ttttacagtt cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt
     1141 tcaccgatgg ttcgtgtctg ggtaatcctg gacctggggg ttacggcgct attttacgct
     1201 atcgcggacg cgagaaaacc tttagcgcag gctacacccg caccaccaac aaccgtatgg
     1261 agttgatggc cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga
     1321 gtaccgacag ccagtatgtc cgccagggga tcactcagtg gatccataac tggaaaaaac
     1381 gtggctggaa aacagcagac aaaaaaccag taaaaaatgt cgatctctgg caacgtcttg
     1441 atgctgcatt ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc
     1501 cggaaaacga acgttgcgat gaactggctc gtgccgcagc gatgaatccc acactggaag
     1561 atacgggcta tcaagtcgaa gtttaagcct gtggtttacg acattgccgg gtggctccaa
     1621 ccgcctggcg aattcgtggc ttgtttttac tctgtttcat cgggtttagc gttaaaggaa
     1681 tagtccgttt ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgcgcattca
     1741 atagttttcc tccgtgtttg ttccacggga gaacgtggaa acggctggcg tgcagcactt
     1801 caaaattcaa caaagagagc cagtccagtt gccgcatcag agtaaacatc cggctgttat
     1861 agggcgaggt ttttcgcaat accggcacaa gtttgcgtaa tcccattaaa ctgatgggat
     1921 tgaagccact aatcaccagc cagccatcat caatcagtac ccgatcggct tcacgcaata
     1981 aacgatgcgg atcggtgcac cacggcaagg tatgtgccag tagacaaaca tcaacggatt
     2041 tatcggcaaa aggaagatga agtggatctg cctgcacctg gacgggcatt ccttgcgcag
     2101 aaacattcac ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc
     2161 caatcttaag cagatgaaaa ccatacattt tagtgaacca cgggttgagc tggcgctcca
     2221 gcgccttgcg ataaagctct ccccagggca aatcgcccca gcaatcagga gccacgacag
     2281 tttgagggac tcttgccggt ttcatcacaa ccttccgttt cacactgaga ggtaatctat
     2341 gaatcttaac agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc
     2401 aggtcgctgc ctgattgtcg atcccggaga cgcagagccg gtattaaaag ccattgccgc
     2461 caataactgg caaccagagg ccatatttct cacccaccat caccacgatc acgttggcgg
     2521 cgtaaaagaa ctggtggaaa agtttccgca aattgtggtg tatggtccac cagagacaca
     2581 agataaggga acaacacagg tagtcaaaga tggcgaaact gccttcgttt tggggcatga
     2641 atttagtgta attgccacgc cgggtcacac tttaggacat atctgttact tcagtaaacc
     2701 ttatctattt tgcggcgaca cgctgttttc tggtgggtgt ggtcggttgt ttgaagggac
     2761 agcatcacaa atgtatcaat cacttaaaaa gttaagtgcg ttacctgacg atacattggt
     2821 atgttgtgct catgaatata cgttatcaaa tatgaagttt gctttgagta ttcttccgca
     2881 cgatttgtcc ataaatgatt attatcgtaa agttaaggag ttacgggcaa aaaatcaaat
     2941 aacactaccc gtaattctga aaaatgagcg gcaaattaat gtttt
//
LOCUS       DAPHDX010000250         2976 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPHDX010000250 DAPHDX010000000
VERSION     DAPHDX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.253"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            203..481
                     /locus_tag="RH058_004432"
     CDS             203..481
                     /locus_tag="RH058_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139899.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSHTFQQRIWR"
     gene            483..1532
                     /locus_tag="RH058_004433"
     CDS             483..1532
                     /locus_tag="RH058_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV3139900.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQVAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQEQHPLQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            1545..1919
                     /locus_tag="RH058_004434"
     CDS             1545..1919
                     /locus_tag="RH058_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV3139901.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMHEEQVKK"
     gene            1916..2737
                     /locus_tag="RH058_004435"
     CDS             1916..2737
                     /locus_tag="RH058_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV3139902.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPLVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            2881..2956
                     /locus_tag="RH058_004436"
     tRNA            2881..2956
                     /locus_tag="RH058_004436"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2914..2916,aa:Ile,seq:cat)
BASE COUNT          753 a          692 c          811 g          720 t
ORIGIN      
        1 gttatggtga tctggtaaaa ttgcataaca ttaaaattta atttatctaa tcgcttttaa
       61 taataagcgt tgtgtttatc ccaacaatct gttgtttgac ttttattcca ttaatgtagg
      121 ggctttacac tggaaccagt ttatttatac tttatacgcc agcctgaaca actggcacct
      181 gctgcgccag cagagacaac cgatggcgca cgataccaaa ttacacaatt ctgatgattc
      241 tgccgtcttt gccagcaggt gcggacggcg ttttcatgca ttcaaatcgg actggttcca
      301 gcatccacca tgcactgaag agcaggctga atggataatt cagtgttacc gcaggcgcgg
      361 atacgaggtt aaaaaagccc ttagcctcga ctaccgtcac tggataatct cagtcagatt
      421 gccttactcc gaacgcccac cgcgtccgtc ccacacattc cagcaacgca tctggaggta
      481 acgtgcgggt attacttcga cctgttctgg taccggaact cgggctggtg atcgttaagc
      541 cgggccgtga atccatgccg gtattccaca atacccggtt actggtggag ccggaaccga
      601 aaagcatgcg taatctgccg tccggggtcg ttcctgccgc tcgccagccg ctggtggaag
      661 acaaaacatt gctgccgttt ttcagtaacg cacgggtgat tcgtgctgct ggtggtgctg
      721 gtgcattgtc tgactggctg ttgcgccata ttaaatcctg ccagtggcca cacggcgatt
      781 atcatcacag cgaaaccgtc attcaccgtt atggtaccgg cgcaatggtg ttgtgctggc
      841 actgcgacaa ccagctgcgt gaccagacat ccgaatcact cgagcaagtt gctcatcaaa
      901 acctgtcagc atggatgatt gacgtcatcg gtcacgcaat aagcggtacg caggagcgtg
      961 aattatctct ggctgaatta tcctggtggg cggtccgcaa tcaggtggcg gacgcgctac
     1021 cggaagcggt attacgtcgt tcgctggggt tgcgtgcgga aaaaatccgc tcaatgtacc
     1081 gtgaaagcga catcgtaccg ggagagcaga ccgccaccag catactgaaa cagcgcacaa
     1141 aaaatcttgc actgttgcct cacgcccacc aggaacaaca cccactacag gaaaagacgg
     1201 tggtaagcat tgccgttgat cctgagtctc cggaatcttt tatgaaacga cctaaacgtc
     1261 gccgctgggt taacgagaaa tacacacgct gggtgaagac acagccgtgt gcgtgttgtg
     1321 gtaagccagc cgacgatccc catcacctga ttggtcatgg tcagggcgga atggggacaa
     1381 aatctcacga tattttcacg ctaccgctgt gtcgggagca tcacaacgag cttcatgcgg
     1441 atccgctggc gttcgaagaa aagcatggtt ctcaggttga tttaatcttt cgttttcttg
     1501 atcacgcctt tgcaactggc gtgcttgggt aaaagaggtg actgatgctc atagatttgg
     1561 ttttacctta cccgccgacg gtgaacactt actggcgacg ccgtggcagc acatatttta
     1621 tctcggagga gggaaagcgt tatcgccggg ctgtggcgct tattgttcgc cagcagcggc
     1681 tgaaattaag cctgtccgga aggctggcga taaaggtgat tgcagagcca ccggataagc
     1741 gtcgtcgcga cctggacaat atccttaaag caccgctgga tgcgctgacg catgcgggag
     1801 tgttaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtggtcag ccagtatctg
     1861 gtggacgtat gggggtgaag atttacccca taatgcatga agagcaggtc aaaaaatgaa
     1921 actggaagat ttaccgaaat actactcccc aaaatcccct tgcctgaccg atgcatcggc
     1981 ctcaacgtca aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     2041 gaatcgggct gagatggggt tttctgcgtt cctggggaaa atgggcatca gtatgaatga
     2101 cagggcgcgg gcaacagaat tactggcaga ttatgcactc agtcggtgcg atcgtgtggc
     2161 ggcgttgaga aaacttccgg cagaaataaa accgttagtg atgcgcatta tggcttcgta
     2221 cgcttttgag gattatgccc gcagcgcagc gagtaaaaag cagtgccctt gttgctatgg
     2281 ggaaaaattt attgaaagcg tagtttttac aaacaaggtc cagtatccgg atggtaagcc
     2341 gccggtatgg gcaaagtgta cgaaaggtgt gtatccgtct tactgggaag aatggaaaaa
     2401 agtcagggag gtggtaaaag ttgcctgtcc ggagtgtggc ggaaagggtg aggtttccac
     2461 cgcctgtaag gattgccgtg ggcgtggtgt cgccattcac cgtgaagagt cggtaaaacg
     2521 tggtatgcct gttatcagag actgccagcg ttgtggtggt cgtggctatg aaagactacc
     2581 atcaacggag gcatttaatg ctatatgcga ggtgacaaac cagataacac gcgcgtcatg
     2641 ggaaaaaaca gttaagaaat tctatgatgc gctggtgacc cggtttgata ttgaagaagc
     2701 atgggctgag cggcagttaa aaaaggtaac taggtaacaa ggttgatttt tccggaatct
     2761 gtggtaaatt cgtcataaag atgggctttt tatacctgac gttagaagag tttctacaac
     2821 ccgccaccga gcgggttttt tattgcggaa ttaattacgg accgttatta ttctgctccc
     2881 ggtcctttag ctcagtggtg agagcgagcg actcataatc gccaggtcgc tggttcaaat
     2941 ccagcaaggg ccaccatcac aaaccgccat gagctt
//
LOCUS       DAPHDX010000251         2881 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPHDX010000251 DAPHDX010000000
VERSION     DAPHDX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.255"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..425
                     /gene="hdeA"
                     /locus_tag="RH058_004437"
     CDS             93..425
                     /gene="hdeA"
                     /locus_tag="RH058_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV3139903.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            541..867
                     /gene="hdeB"
                     /locus_tag="RH058_004438"
     CDS             541..867
                     /gene="hdeB"
                     /locus_tag="RH058_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV3139904.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            931..1578
                     /locus_tag="RH058_004439"
     CDS             931..1578
                     /locus_tag="RH058_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV3139905.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1620..2150)
                     /locus_tag="RH058_004440"
     CDS             complement(1620..2150)
                     /locus_tag="RH058_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3139906.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2306..2872)
                     /gene="slp"
                     /locus_tag="RH058_004441"
     CDS             complement(2306..2872)
                     /gene="slp"
                     /locus_tag="RH058_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV3139907.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          839 a          579 c          645 g          818 t
ORIGIN      
        1 atcaacatga catatacaga aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac
       61 ggctctttca ctttatagtt gaggatatta cgatgaaaaa agtattaggc gttattcttg
      121 gtggtctgct tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata
      181 acaaaaaacc ggtcaactcc tggacctgtg aagatttcct ggctgtggac gaatccttcc
      241 agccaactgc agttggtttt gctgaagcgc tgaacaacaa agataaacca gaagatgcgg
      301 ttttagatgt tcagggtatt gcaaccgtaa ccccagctat cgttcaggct tgtactcagg
      361 ataaacaagc caactttaaa gataaagtta aaggcgaatg ggacaaaatt aagaaagata
      421 tgtaattccg ggaatgcgtt acatcgtact tccttgcata ttgaacaggc cggaatatct
      481 tctttaaaag cagctattcc tcctgttcat atataatctc tatattgaat gggttacaaa
      541 atgaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtca
      601 ctggtgaacg cacaatctgc gctggcagcc aatgaatccg ctaaagatat gacctgccag
      661 gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa
      721 gaaacagtat ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt
      781 cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat
      841 caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag
      901 ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat acgtcttata
      961 ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca
     1021 gggttacgca cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa
     1081 tatggttttg ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct
     1141 gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa
     1201 aatattgtag gtctgacgac ggcagcggat atctgggtga ccgccgccat aggtatggtt
     1261 attggcagcg gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg
     1321 gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca
     1381 ttagtgaacg gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaacg
     1441 gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt
     1501 catgccacaa cgtcgataga agacctgttg cgattattaa aaggtatggc aggggtgaaa
     1561 ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt
     1621 cacaccagat aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg
     1681 atcatcatga tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc
     1741 agaatatctt ccgttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc
     1801 gaaagtgaca cagccgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa
     1861 tcaatgatag tgatagtccg cggaacaaag gtcaccggca ctttccccag gcagcgttta
     1921 atattaaagg gggagaaaat aatcacatgg acaggtttta atcgttcaat ttgagtgaga
     1981 aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc
     2041 aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg
     2101 tttttcatcg cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc
     2161 tggtgcggac attaacttta taatcagttt gccaaaggta attaacagga cagggcttat
     2221 aataataata gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag
     2281 aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg
     2341 cattggtgta gtaaggcgca ccccagcccg gttccggcca gaatgcacca tagccgtaat
     2401 cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca
     2461 tattcacttc caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa
     2521 tggtaccgag gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt
     2581 gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca
     2641 acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct
     2701 tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa
     2761 aacttttttg aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg
     2821 cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat
     2881 c
//
LOCUS       DAPHDX010000252         2861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPHDX010000252 DAPHDX010000000
VERSION     DAPHDX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.256"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..704
                     /gene="comR"
                     /locus_tag="RH058_004442"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="RH058_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV3139908.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(766..1305)
                     /locus_tag="RH058_004443"
     CDS             complement(766..1305)
                     /locus_tag="RH058_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV3139909.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RH058_004444"
     CDS             complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RH058_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV3139910.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGVNGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          687 a          785 c          699 g          690 t
ORIGIN      
        1 ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccagattc aggagatatc gccaatactt
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctaatgcaga
      661 ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccattac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa cagtggcgac
     1021 atctttacca cgaccaccac caaactgatg ccctatcacg ccgccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccccgtt caaacacgtt cagactggcc actgccgcta cgcccagcgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa cacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaagg attcggagga gtctgaagga atgcctgatg
     1441 cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggatgagg
     1501 cgtttatgcc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa
     1621 tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg cgtcaggtta cccatcaggc taccgacggt ggagaagttc gacagcgata
     1741 ccagcgaacc atgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca
     1801 gtatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaatg tctggatcgc
     1921 gggtggtttg cagcgtcggt tccaccacca gctggttgat acggttagtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccacaccatc agatcagcct
     2041 caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca gagatacgtg
     2161 gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcaggcc tttgtaaccg tagctgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg ccatttacgc
     2341 ccaggttggc ggagtatttc aggaagagat tcagcatctc ctggtgaaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggcgtattg aaatcgttag
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 gttcaccttt ctcatcgcgc agttctgcaa tagtgattgt tttcgcttca cgatcaatat
     2581 caatgacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttacgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatctttt
     2821 tcaatggcgt agtcaacgtg acccccttaa atttattaac c
//
LOCUS       DAPHDX010000253         2832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPHDX010000253 DAPHDX010000000
VERSION     DAPHDX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.257"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            131..2410
                     /gene="ydeP"
                     /locus_tag="RH058_004445"
     CDS             131..2410
                     /gene="ydeP"
                     /locus_tag="RH058_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV3139911.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKYSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2658..>2832
                     /gene="safA"
                     /locus_tag="RH058_004446"
     CDS             2658..>2832
                     /gene="safA"
                     /locus_tag="RH058_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDV3139912.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHF"
BASE COUNT          758 a          654 c          687 g          733 t
ORIGIN      
        1 aatagattaa aaatagaaag agagtagtcc cctactctta tatatccatg ttggcgataa
       61 tccctttgta tatactgtgc atcatcgcta ttacaaatcc taataattca tttccacaca
      121 ggataagtag atgaagaaaa aaattgaatc ctaccagggt gctgccggtg gttggggtgc
      181 tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccagg atgttattgc
      241 catgtttgac atgaataagc cagagggctt tgactgtccg ggttgtgcat ggccagatcc
      301 taagtacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg cctgggaagt
      361 cacggataag caggtaaatg cctctttctt tgctgagaat acggttcaat cattacttac
      421 ctggggagat cacgagcttg aggctgcggg acgtctcact cagcctttga aatatgatgc
      481 tgtcagcgac tgttacaagc cattaagctg gcaacaagct ttcgacgaaa ttggcgcacg
      541 ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcgggcc gcacttccaa
      601 tgaagctgcc tttctttatc agctttttgc ccgtgaatac gggagtaata actttcccga
      661 ctgctccaac atgtgccatg aaccgaccag tgtgggtttg gcagcgagta tcggtgtggg
      721 taaagggact gtgttgctgg aagactttga gaagtgcgat ttagtcattt gcattgggca
      781 taaccctggt acaaaccacc cccgcatgct gacttcgttg cgcgctttag tgaaacgggg
      841 agcgaaaatg atcgccatca atcctctaca ggaacgtggc ctggagcgat ttaccgcacc
      901 gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggctagtg cctactataa
      961 cgtgcgcatt ggtggcgata tggcgttgct caaggggatg atgcgcctgt taattgagcg
     1021 cgatgatgct gcaagtgccg caggtcggcc ctcattgctg gatgacgaat ttattcaaac
     1081 gcataccgtc ggctttgacg agctacgccg tgacgttctc aattccgagt ggaaagatat
     1141 cgaacgtatt tctggattaa gtcagactca aatcgccgaa ctggctgatg catatgccgc
     1201 tgccgaacga accattatct gttacggaat ggggatcact cagcacgaac atggtaccca
     1261 gaatgtacag caactggtca atctgctgtt gatgaaaggt aacattggca agcctggtgc
     1321 gggtatctgc ccactacgtg gccactctaa tgtacagggc gaccgaaccg tcggtatcac
     1381 cgagaaaccg tctgcagagt ttctggctcg cctgggtgag cgctatggct tcacaccacc
     1441 tcaagcacct ggacatgctg caattgccag catgcaagca atatgtacag ggcaggctcg
     1501 agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg aagcgagcgc
     1561 tgtaccgtta acgcaattag atctggcggt acacgtagcc actaagctta accgctctca
     1621 tctgctgacc gcacggcata gctatattct gccggttctg gggcgtagcg agattgatat
     1681 gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga ttcatgcctc
     1741 gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgctg tggtcgcggg
     1801 aatcgcccag gcaacactac cccagagcgt ggtagcctgg gagtatctgg tggaagatta
     1861 tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag ttcgccgact ataaccagcg
     1921 catccgtcat cccggtggtt ttcacctgat aaatgcagct gctgaaaggc gctggatgac
     1981 gccgtcaggt aaggctaatt tcattaccag caaagggctg ttagaagatc cctcttcagc
     2041 gtttaacagt aagctggtca tggcgacagt acgcagccac gatcagtaca acacgacgat
     2101 ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg tctttatgag
     2161 tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc ttattgcgct
     2221 tacgccagac ggtaagcgca gctcacgccg catggataga ttaaaagtgg tcatttaccc
     2281 tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc taactcttga
     2341 taaccacgat ccattaagtg gcattcctgg ttataaaagt attccggttg aattagaacc
     2401 atcaaattaa ttcgcctcct catttcttct gctgtcatcc gcacagcaga agaatttctc
     2461 attgactatt atcgcgcaat ttgctcacat tgattaaatt aaaccacaca ctataagata
     2521 taaacctctg cctacagctg taagaaactc cgctcagtac tgaagtgcca gtcctatttc
     2581 ctcttttctc cagcctgtta tattaagcat actgattaac gatttttaac gttatccgct
     2641 aaataaacat atttgaaatg catgcgacca cagtgaaaaa caaaatcacg caaagagaca
     2701 actataaaga aatcatgtct gtaattgtgg ttgtcttatt actgaccctt acgttgatag
     2761 ccattttttc ggcaattgat cagctgggta tttcagaaat gggtcgcatt gcaagagatc
     2821 ttacacattt ca
//
LOCUS       DAPHDX010000254         2817 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPHDX010000254 DAPHDX010000000
VERSION     DAPHDX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.258"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..948
                     /locus_tag="RH058_004447"
     CDS             <1..948
                     /locus_tag="RH058_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139913.1"
                     /translation="HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASV
                     FWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTV
                     RLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAV
                     ASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDR
                     VKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNML
                     IKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            1024..1479
                     /locus_tag="RH058_004448"
     CDS             1024..1479
                     /locus_tag="RH058_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139914.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            1558..1791
                     /locus_tag="RH058_004449"
     CDS             1558..1791
                     /locus_tag="RH058_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV3139915.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            1891..2709
                     /locus_tag="RH058_004450"
     CDS             1891..2709
                     /locus_tag="RH058_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3139916.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          681 a          736 c          747 g          653 t
ORIGIN      
        1 catctgactt atactcaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact
       61 tatgatattg ccgagggttc ggttgtcagt ctggcggccg gagggatcag ttttgccctg
      121 acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct
      181 gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactc
      241 caccggctca gtgttgttca gggtatgctg aagcacattg atttttcgac tgccagcccg
      301 actgtccgac tggctcttca gaaggggacc ggtgcaggaa atatcagtgc cctgaacaaa
      361 gtgatgaagg gtacgctggt gacatctctg gcactggtag ctgtcacaac cggccctgac
      421 atgatcaaaa tgttgcgggg acggatctcc ggtacgcagt tcatcaggaa tcttgccgtg
      481 gcatcttccg gtgtggcagg tggagctgtc gggtcagtgg cgggcgggat actgttcagt
      541 ccactgggac catttggtgc actgacaggg cgtgtggttg gcggtgttct ggggggaatg
      601 attgcctccg ctgtatcagg aaaaattgcc ggagcgctgg ttgaagaaga tcgcgtcaaa
      661 attctggcaa tgattcagga gcaggtgaca tggcttgccg gcagtttcct gctgaccgga
      721 catgagattg aaaatctgaa cgagaatctg gcccgtgtta tcgatcagaa tgctctggag
      781 atcattttcg ccgccggtat acaacaacgg gccgcgacca atatgttaat caaaccactg
      841 gtggtcagta tcatcaggca acgccccgtc atggaatatg atgcatccca tctcggcaat
      901 atggttaacc gactggaaga agcattaccc ccggagttac cggcataacg gctattctgc
      961 gccttttacg ctttatcacc tgatttgtca gtgtcatcgt catgactcag agaggtaaat
     1021 accatgattc acctgttcaa aacctgcatg attaccgcct tcattctggg gttaacgtgg
     1081 tctgccccac tccgggcaca ggatcaacgt tacatcagta tacgcaatac agatacgata
     1141 tggctcccgg gaaatatttg tgcttaccag ttccggctgg ataatggcgg aaacgatgaa
     1201 ggatttggcc ccctcaccat cactctgcaa ctcaaagaca aatatggtca gacgctggtg
     1261 accagaaaaa tggaaacgga agcttttggt gacagtaatg ccacgcgaac cacagacgca
     1321 tttctggaaa cggagtgcgt ggaaaatgtc gccacaaccg aaatcattaa agcaactgaa
     1381 gaaagtaacg gccatcgtgt cagtctgccg ttatcggttt tcaatcccca ggactaccat
     1441 ccactgctga ttaccgtttc cggaaaaaac gttaactgat ccctgaagtc agtttcatgc
     1501 ttcagcctct gtcacaacgc gtggcagagg ctttttattt atcaggagaa aacagatatg
     1561 ccgggttaca cagagtatgt cctggctgaa ggatctttct catacgggca ggcggttgct
     1621 gtcataaccg cttaccgcaa tgtgtttatc caggatgacc cgggaatgca tttccgtcgg
     1681 gttatccgca acgcggaagg tcagcgacgc tggcgctgcc ggaattctga acccgatgcc
     1741 ggaaaggtgc ttaatacgcg gctcgccagt gacggtcttc tcagacaata aacgtcattc
     1801 atttcaccca tcaggccgcg tcttctccgg gagacgtggc tttttcattt attacaccag
     1861 taaatcttaa ccaccgataa ggagcaaatt atgcgactag ccagtcgttt tggttatgcg
     1921 aaccagatac gccgtgaccg tccgctgaca cacgaagaac tgatgcacta tgtgccgggg
     1981 attttcgggg aagataaaca cacgtcccga agccagaact atacgtacat ccccaccatc
     2041 accgtactgg aaagcctgca gcgggaaggc tttcagccat tcttcgcctg ccagacccgt
     2101 gtgcgcgacc cgggccgccg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga
     2161 gagataaacg gagaacatgt ccctgaaatt attctgctca actctcatga cggtacctcc
     2221 agctaccaga tgctgccggg ttacttcagg ttcgtctgcc agaacggatg cgtctgtggt
     2281 cagtctctgg gggaagtgcg tgttccgcac cggggaaatg tggtggacag agtcattgaa
     2341 ggggcttacg aggtggtggg ggtttttgac cggattgagg aaaagcgtga tgccatgcag
     2401 tcgctgattc tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt
     2461 tatggtgacg aacatcagcc cgtcaccacc gccgacattc tgacgccacg acgccgggag
     2521 gattacggta aggacctgtg gagtgcttat cagaccattc aggagaatat gctgaaaggc
     2581 ggaatttcag gtcgcagcgc taaaggaaaa cgtatccata cccgtgccat tcacagcatt
     2641 gacactgaca ttaagctcaa ccgcgcattg tgggtgatgg cggaaaccct gctggagagc
     2701 ctgcgctgat tcagccatta ccctgaaaac cacgcattta tttgtccttt aaggagtcat
     2761 ttcatgaaaa ccatttcaca taattccacc acatcttctg tttccgtaac cgctgca
//
LOCUS       DAPHDX010000255         2795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPHDX010000255 DAPHDX010000000
VERSION     DAPHDX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.259"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..1342
                     /gene="nhaA"
                     /locus_tag="RH058_004451"
     CDS             176..1342
                     /gene="nhaA"
                     /locus_tag="RH058_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV3139917.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1408..2307
                     /gene="nhaR"
                     /locus_tag="RH058_004452"
     CDS             1408..2307
                     /gene="nhaR"
                     /locus_tag="RH058_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV3139918.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2346..2795)
                     /locus_tag="RH058_004453"
                     /pseudo
     CDS             complement(2346..2795)
                     /locus_tag="RH058_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          661 a          621 c          704 g          809 t
ORIGIN      
        1 tagggtcacc cgttagcgct tacagccgtc aaaaactcat ctcaccgctg atggcgcaaa
       61 tcctttaatt gctcgtaaaa aacgagttat tcctacacta taatctgatt ttaacgatga
      121 ttcgtgcggg gtaaaatagt aaaaacgatc tattcacctg aaagagaaat aaaaagtgaa
      181 acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctta tcattgccgc
      241 tatcctggcg atgatgatgg ccaacagcgg cgcaaccagt ggatggtatc acgactttct
      301 ggagacgccg gttcagctcc gggttggttc actcgaaatc aacaaaaaca tgctgttatg
      361 gataaatgac gcgctgatgg cggtattttt cctgttagtc ggtctggaag ttaaacgtga
      421 actgatgcaa ggatcgctag ccagcttacg ccaggccgca tttccggtta tcgccgctat
      481 tggtgggatg attgttccgg cattactcta tctggccttt aactatgccg atccgattac
      541 ccgcgaaggg tgggcgatcc cggcggctac tgacattgcc tttgcacttg gtgtgctggc
      601 gctgttggga agtcgtgttc cgttagcgct gaagatcttt ttgatggctc tggctattat
      661 cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact tatcgatggc
      721 ctctcttggc gtcgcggctg tagcaattgc ggtactcgcg gtattgaatc tgtgtggtgt
      781 acgccgcacg ggcgtctata ttctggttgg cgtagtgttg tggactgcgg tgttgaaatc
      841 gggggttcac gcaaccctgg cgggggtaat tgtcggcttc tttattcctt tgaaagagaa
      901 gcatgggcgt tctccagcga agcgactgga gcatgtgtta cacccgtggg tggcgtatct
      961 gattttgccg ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggtg tcacgctgga
     1021 tggcttgacc tccattctgc cattggggat catcgctggc ttgctgattg gcaaaccgct
     1081 ggggattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcgcatc tgcctgaggg
     1141 aacgacttat cagcaaatta tggcggtggg gatcctgtgc ggtatcggtt ttactatgtc
     1201 tatctttatt gccagcctgg cctttggtag cgtagatcca gaactgatta actgggcgaa
     1261 actcggtatc ctggtcggtt ctatctcttc ggcggtaatt ggatacagct ggttacgcgt
     1321 tcgtttgcgt ccatcagttt gacaggacgg tttaccgggg agccataaac ggctcccttt
     1381 tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca cttgtattac
     1441 ttctggcatg tctataaaga aggttccgtg gttggcgcag cggaggcgct ttatttaact
     1501 ccacaaacca ttaccggaca gattcgagcg ctggaagagc gcctgcaagg caaattattt
     1561 aaacgcaagg gacgtggtct cgaacccagc gagctgggag aactggtcta tcgctatgcc
     1621 gataaaatgt tcaccttaag ccaggaaatg ctggatattg tgaactatcg caaagaatcc
     1681 aatttattgt ttgacgttgg tgtggctgat gcactttcca aacgcctggt cagtagcgta
     1741 cttaacgccg cagtggtaga aggcgagccc attcatcttc gctgcttcga atccacccac
     1801 gaaatgctgc tggagcaatt aagtcagcat aaactggata tgatcatttc tgactgtccg
     1861 atagactcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt
     1921 ttctggtgta caaatccacc accagaaaaa ccgttcccgg cttgtctgga agaacggcga
     1981 cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc
     2041 cagggattaa acgtagaaat cctcggcgag tttgatgatg ccgcgttgat gaaagctttt
     2101 ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga cttttatgcc
     2161 gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta ccatgctatt
     2221 tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac ggattattct
     2281 gcgcttttta gtccagcggc gcgttaatcg gcacctcccc ccaaagttaa ggtgggggag
     2341 atagattagt tgtacataac cacgattttg actcggctca ttatttgccc gcttgagaca
     2401 ttatttccat atgtacgcgg gcgaataaat agaggaatct gattacttcc ttcatgggga
     2461 tgctgaaaag agtagtaatt gcttttacca tactggattt cattcgtacc atcattggag
     2521 agttaaaagc ctacttgacc cgcaccgtcc tcactgtttt ctaactcatt ttttaatatg
     2581 ctggttcctg actcctgtat cgtcactggt tcaaagtaat attcaacatc actgggttca
     2641 aaaactaatt caccttgctc gccctggatg cagtcaaatc ttatatgatg aaactgataa
     2701 ttttcattat tgatacgaga cagtacaaat cgtccagtgt cgatggattg atcttcaaaa
     2761 ccacaggtca ttaaatttac ctgtaaaata gtatt
//
LOCUS       DAPHDX010000256         2765 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPHDX010000256 DAPHDX010000000
VERSION     DAPHDX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.260"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1940
                     /locus_tag="RH058_004454"
     CDS             <1..1940
                     /locus_tag="RH058_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDV3139919.1"
                     /translation="TLIILPIGLQAETSHSSMAKDTITVVATGNQNTVFETPSMVSVV
                     TNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVLVLVDGIRQLSD
                     MAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADFLPPGETNGLSL
                     WGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAPNKEKPAALFAK
                     GSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDVQFWYQYAPVDN
                     SLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFPGAHQLSYGAEY
                     YRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDRYKSFNPRAGEL
                     KAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRKGKPNYWVPNLN
                     LKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVDMKRMRSYSYNV
                     SRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTLISDISIPVGHG
                     GVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEASVTLDNAFNKLA
                     MNGKGVPLSGRTVSLYTRYQW"
     gene            1947..2738
                     /locus_tag="RH058_004455"
     CDS             1947..2738
                     /locus_tag="RH058_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDV3139920.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGVQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLQVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
BASE COUNT          785 a          645 c          668 g          667 t
ORIGIN      
        1 gtacattaat catactcccg atagggttac aggcagagac cagtcattct tctatggcta
       61 aagatacaat caccgttgtc gcgacaggaa atcagaacac ggtatttgaa acgccgtcaa
      121 tggtcagtgt cgtcacgaat gacacgccat ggagtcagaa tgcggtcaca tcagccggca
      181 tgctgaaagg tgttgccggt ctcagccaga ctggtgcagg acggaccaat gggcagacct
      241 ttaatctacg cggctatgac aaaagcgggg tacttgttct tgttgacggc atccgtcaac
      301 tcagtgacat ggcaaaaagc agtggcactt atctggatcc ggcactcgtc aaacgtatcg
      361 aagttgtccg cggaccaaac tccagtctgt acggcagtgg cgggctggga ggtgttgtgg
      421 acttcagaac tgccgatgca gcagatttcc ttccccccgg agagacaaac ggtttaagtc
      481 tgtggggaaa tatcgccagt ggtgaccaca gcacaggctc ggggctcacc tggttcggta
      541 aaaccggaaa aacagatgcg ctcctttctg tcattatgcg taaaagaggt aatatctatc
      601 aaagtgatgg tgagcgcgca cctaacaagg aaaaacctgc agccctgttt gcgaaaggct
      661 ctgtcggtat aacagacagt aacaaagcag gtgccagctt gcgtctctac cggaataaca
      721 ccactgaacc gggcaatccc actcagacac atggtgacag cggcctgcgt gacagaaaaa
      781 cagtacaaaa tgacgtacag ttctggtacc agtacgctcc tgtggataac agcctcatca
      841 atgtaaagtc aactttatat ctcagtgata tcactatcaa gacaaacggt cacaacaaaa
      901 cggcagaatg gagaaacaac agaacctccg gtgttaatgt tgtcaacagg agtcatactc
      961 tgatttttcc gggagcccat cagttaagtt atggcgctga atattaccgt cagcagcaga
     1021 agccagaagg ctctgccaca ctatatccgg aaggaaacat tgactttaca tcgttgtatt
     1081 tccaggatga aatgacaatg aaaagctacc cggttaacat tatcgtcggt tcccgctatg
     1141 accggtacaa gagcttcaat ccccgtgccg gagaactgaa agccgaacgc ctgtccccaa
     1201 gggcggcgat ttcagtctca ccgacagact ggctgatgat gtacggctcc atatcctctg
     1261 cattccgtgc ccccacaatg gcagaaatgt acagggatga tgtacatttt taccgcaagg
     1321 gtaaacccaa ttactgggta cctaacctta atctgaaacc agaaaataac attacccgtg
     1381 agattggcgc aggtattcaa ctggacggcc tgcttacagg caatgatcgg ctgcagttga
     1441 aaggcggata tttcggaacg gatgccagaa actatattgc cacgcgcgtg gatatgaaac
     1501 ggatgcgttc ttattcttat aatgtatccc gggcccgtat ctggggatgg gatatgcagg
     1561 gaaattacca gtctgattat tttgactgga tgctttctta taaccgcacg gaaagtatgg
     1621 atgccagcag cagggaatgg ctgggctccg gcaatcctga cacactcatc agtgacatca
     1681 gtatacctgt tggtcatgga ggagtttctg ccggatggcg tgctgaactt tcggcagcag
     1741 ccacgcatgt gaaaaaaggc gatccccatc aggctggcta tgccgtacat tccttttcac
     1801 tgtcttataa gcctgtaagt gttaaaggct ttgaggcatc agtgactctg gataatgcct
     1861 tcaacaagct tgccatgaat ggcaaaggag tgccgctttc aggcagaact gtcagtcttt
     1921 atacccgtta tcagtggtaa caaatcatga tgaaaaatac aggttatatc ttagctcttt
     1981 gtctgacagc atcggggcat gtcctggccc acgatgtctg gattacaggt aaacaggcag
     2041 agaacaacat taccgcagag attggttatg gtcataattt cccctcaaag ggcacaattc
     2101 ctgacagaag gaatttcttt gaaaatcccc ggatttataa cgggaaagag acaataacac
     2161 tgaagccagc gtccacggat tatgtctata aaactgagtc tgcaagcaaa gataatggct
     2221 acgttttgtc aacatatatg aaaccgggat actggtcgag aacctcttca ggatggaaac
     2281 cggtcagccg ggagggcaga aatgatgtgg cttactgtga gtttgtcact aaatatgcga
     2341 aatcttttat tcctggtgta cagcagatgc cagcacaact ctatcagtct ccaacggggc
     2401 atgagcttga aatcattccg ttatccgata taagtcgttt cagtgaagat gtgaagctgc
     2461 aagtcctgta taaaacgtcc ccgctcgccg gagctatcat ggagcttgac tcggtcagtt
     2521 atctgaaatc atcccgtcat actcatgcag ttgagcacaa acatcctgtt cataaagcag
     2581 aactcacctt tgtaactaat gaggatggta tcgtcacagt accttctctt catattggac
     2641 agtggctggc gaaagtccaa aataagaaaa gttttcagga caaaagcctg tgtgatgaaa
     2701 ctgtcgatgt ggcaacttta agtttctccc gaaattaaca atactattcc ctgccctgaa
     2761 attgt
//
LOCUS       DAPHDX010000257         2740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPHDX010000257 DAPHDX010000000
VERSION     DAPHDX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.261"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(28..528)
                     /locus_tag="RH058_004456"
                     /pseudo
     CDS             complement(28..528)
                     /locus_tag="RH058_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(708..1821)
                     /locus_tag="RH058_004457"
                     /pseudo
     CDS             complement(708..1821)
                     /locus_tag="RH058_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(1858..2355)
                     /locus_tag="RH058_004458"
                     /pseudo
     CDS             complement(1858..2355)
                     /locus_tag="RH058_004458"
                     /inference="COORDINATES: protein motif:HMM:NF024728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            complement(2352..2510)
                     /locus_tag="RH058_004459"
                     /pseudo
     CDS             complement(2352..2510)
                     /locus_tag="RH058_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          577 a          817 c          601 g          745 t
ORIGIN      
        1 aggcgacaac taccttgaaa aacgccgtta ctcaccgatc tccttcacgg taatacgcac
       61 cttaatatca atagtccatc tggcacctgt ttgttcttga gacgacgata aatttcgcaa
      121 cgtgatcgat gttccgttat ccagctttgc accccggccg aacctctttc agcggtgtat
      181 cgcctgccag ttgctgtgcg taattgtgga tctgtttatc cgtcaaattc tggctttcaa
      241 acactttcgt cgtaccataa cggttactta ccaaactctg cctgatagtc tgcccatcca
      301 ccgttaactc tccaaccggg tgacttttat catatatttt actcagttca tcaaatcgtt
      361 gttgctgaac ctcctgatga cgggcttttt cttcattatc tggtccccat cccccggtgt
      421 tccagcacct gcactaccaa gctcggcttt ctcattgtcc gttgcgccag ccagttcttt
      481 accctggtta ggctgtttat cttcgtctgt attgtcgcca ttcagcacat gccccggtcc
      541 ccgggtgcca tcgcgactcg gatcacgtac agcacactgt tcccgtgagc cttcacaacc
      601 accaccggta tcccagctat tatcattccc ggtatcctta ctggcattac ctgtagcacc
      661 agctattgct gctgccgtat tagctgctgt ttcagtggct atcacgttca gcatattgtt
      721 ttcagccgag ttcctacctg cctgtgcacc cgtaccggca gcacttgtac tgttgccggc
      781 aagcccgccc gcaatacctg aggcaatggt gaccagcatg ctgatttgct gacgttcctg
      841 ttcactcagg ttatcggtac aaccaccgta gtagttatca atgatatacc gggcagcaag
      901 ttcaccactg aatgcccctg cagcaccgga agccgcattg tttccgccca gttgtgcagc
      961 aagcgctccc cagactgcgt gagccatcag gtttgcttcc cggttgtcac cggtagcctg
     1021 ttttataagc tgtgccactg ctggattcag accgccagcc agtacctgcc ccggattccc
     1081 tgcattcagt ccgcccagca cgcccgtgat ggcctgtacc accatctgcg gcgtactgcc
     1141 cgtaccgtag cctttcatga cctctctgta ttccggggtc tcccgcaact gtgcatcact
     1201 cagccctgga tatttcgccc tggctgcttt catcgcttca gtttcgccat acgtcatgac
     1261 gatgttactc atctgaccgc taatctggct gataacctgc gcttcctgaa gacgcttctg
     1321 ctctttctcc ctgtcaaata tcggtgcaat gctgccgttg gcattttccg ggtcccggct
     1381 gagcccggca atatcctgct tctgactctc cttatcacgg ataatgatat ttccgccgct
     1441 gatggcggat gacgtggtac cttcagcatg tccactgctg ccggacatac cggtaagtgc
     1501 gttcgccgca ccggctattg catttgaggc caccatctgg ctggctgaca tccctgagcc
     1561 acccgatgaa ctgattccgg tatggctggc tttatagtct gcctggttat ggatatccga
     1621 ccagcccagc gtgccggtac tgaggctgtt tttgtcatcc gttgccgtgg acgcaattac
     1681 cgcaccattg agctgagtgt gattccccac agtgacatca aacccgtctt ttccggcgta
     1741 gataccactc tgttcccgga cagaatcgta attgctttta attttgtcct gactgatgct
     1801 ggcatagccg ctgccggaca ttgaaccaaa cgtgaagctg ccacctgcgg ccacgctgtt
     1861 ctgtctgctg tcgtaacggt cactgtcctg aaggctcgaa atggtcaggt tgttgccgac
     1921 atctgctgtg actttctcgc cactgacctg agcccctgac agggtggtat cacgtccgct
     1981 tgtgagggag acatttttac ccgcatccag cgtggtttcg gtccaggtcg tgccgttgcc
     2041 tttctcactg cctttggagc cgttaatgct ggcgaagata ctcagcccgc caccattagt
     2101 accaccacca aagctgacgc ccacgccgcc acctttgctg ctgttcttcc cggtggtctg
     2161 tcgggtgtca gcggcggcag ccagcagaat atcgttctgt gcattcaggc tggtgtcatt
     2221 gccggactta atctggctcc cggtaatgag aatatcgccg ctgttgttat ggtccttatt
     2281 tttccccgtg gcagtgacgg agacattgtt accggcgctg agagttgagc cggagacgat
     2341 atcgtgctga tatatcccat aacaagatgc tccctgagcg cattaatcac tttctcactg
     2401 tgcatcccgg atatttcaat caataaccag aaatgccttt ctgatacttc cccgggcagc
     2461 agaatcccgg accgctcttt caccagatgc aaacaaacct ccgaattcat aaccccgttt
     2521 actctgacag ggaaacaaac tctcagcatc agcgccggaa tgaacaacgg taatcccctg
     2581 cctcgccacc aaaattacca tggagtttaa tgctgtaaat attattattg acgatctttt
     2641 taaccaaata acaacaatta aacaggttat aacaccacac cagtgaaaaa aaatacagca
     2701 ctaaaatcca gaaatcatat aaaatacaga taaaaagatc
//
LOCUS       DAPHDX010000258         2707 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPHDX010000258 DAPHDX010000000
VERSION     DAPHDX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.262"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1878)
                     /locus_tag="RH058_004460"
     CDS             complement(<1..1878)
                     /locus_tag="RH058_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV3139921.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ET"
     gene            complement(1922..2605)
                     /locus_tag="RH058_004461"
     CDS             complement(1922..2605)
                     /locus_tag="RH058_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV3139922.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          629 a          634 c          603 g          841 t
ORIGIN      
        1 cgtctcagtc aggttagaga tgttggtggt gttagtggca atattggaag tgttattggc
       61 gatattggtg gtattcgtcg ccacagcatc gttggtggtt ttcagctgag agccgttaac
      121 agcatcagtg ctgtcagccg tcaggtcggc atctttaacg ttggtgattt tgctggtggt
      181 atcctggccg tgagctgcac tgaatgcctt tttagtttcg ttccagagca gggcatcagc
      241 ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg gtggtattgg tggcaatatt
      301 tgccgtattc gtggcaatgt tgctggtatt agtagcgata ttggcggtgt tggcttcgac
      361 atcgtcattg gtagctttca gttgggaacc gttaaccgca tccttactgg aggctgaaat
      421 cgtgccatcc gcaacatcgg tgattacgct ggcagttggg ctactagtac catgagcagc
      481 gctgtatttg cctttggttt ggtcccattg cagggtgttt tcatcaagta cagcgagcgc
      541 agcccctacg ttatttttgc taccattttt taaattgtag gttggtgctg taacagtacc
      601 gtcatcaaca tctactgcag cccctcctcc aagccttttt gctaccgagt cgctaatggc
      661 ataaagctgt gaaccattaa tggcatcgta actgtcagac ttgatcgcgc catttttcac
      721 attaacgatc ttgcgtttta atgaactatt gccgacagag acggtattgt ctaaatccgc
      781 aatggaatct gcgcccagcg cgacagagtt aataccgttg gcactgctat tactaccaag
      841 cgcaagactc ttatcaccag ttgcattact ggctgtgcct aaggcaatgg tgtttaagcc
      901 tctcgactgg ctaccattac ccagagcgat ggcatccacg ccatcagctt tgttgccctg
      961 gcctatggcg attgcattat ctgccagagc ctggctgcca tttcctaatg cgatagtatt
     1021 gactttatcg gcaaggcttc cgaaccccat tgccatgcta ttagtccctt ttgccttcgc
     1081 attattgcca atagcagttg cattttcagc ggcttcggtc tcagcaccta tcgcaatagc
     1141 gttcgctgcg ctggcaagtg attgtctgcc aaatgcgagg ctgtcagtac cgctagctac
     1201 actgctacgc cccagggcaa ttgcgttttc ttcagatgcg acgctacttg ctcccagcgc
     1261 cattgaatac gcttttgacg cattggcggt gtcgccaaga gcaatactca taatctcagt
     1321 agctttagtg gcatttccca gggcaatggc gtttgctcct tccgctttgg aagagtcccc
     1381 catagcaata gacaatttac ccaatgcttt tgaataacgg ccgagggcca ttgagtattg
     1441 gcccaatgaa tacgaagagg cacccagtgc tatacttcta tcgccttctg atattgcact
     1501 aaccccaaag gccattgatg caccgccaat cgcatgggtt tttgacccga tggcagagct
     1561 atattggcct tcagctatag cgtcataacc cacagcagta ctggaaccac tggtgttcat
     1621 aaaagtattt gctttcgccc ctttgcctat agcaacccag ccgtcgccag ctgatccact
     1681 accgtaatca acaccctgac cgttgtcatt cccggcattc gccaatgccc caaacgacga
     1741 caacattcca cccgcaacca gcgcagaaat taacagctta ctgcgcccag atttcttgcc
     1801 acggcttttt gccgtttcgc tggtaacagt ataattccct gtcgcagggt tccagataac
     1861 tttaaatatt ttgttcatta atgatgctcg ctattccttg ttataagcgt actaactgca
     1921 tttatttttt gcttcttttc tgccgatttt cagcagaggg aagattgtcg aactgaatat
     1981 tttccatgtt gatggctaac ttcagatatc ccaggttatt gggcgataca cccttcaggg
     2041 tcaaattaat gtcttgccat tgacctggta ataatttcaa tgtggatttc attgatgtgt
     2101 tgaaaagtaa ttgttgccac cgggcaaact ctttggcatc gacaggtttt tcaccacccc
     2161 atgtcgcatt aaaaccaatg tttgagatag gtgtacttaa taagttgacc agtgagatat
     2221 gaataacact accattctct gtggcgtcaa tggataacac tcgacccgct aaagccaccg
     2281 aaccgttgtt caggatgaaa tacttcgttt ttgctgggtt aaactcggta aaaataaaag
     2341 tattttgttg gtctttaacg gatttaatta cgtgagttaa agtctttgta ctgtttatta
     2401 tgccagtttg ttgtttttga atagtttcga gttgactctt tagctgtgct tgttgttttt
     2461 caagtagatc aattttattg ttatgttctt cttgttgttt caataaacta ctttctggct
     2521 tgccttcttc aatgtagtca catcccgtta atgcgcagag aactgaaaca gtgacagcac
     2581 tgaaaaaaat cttcttcaag ttcatgtaaa aatccttatt tcctccgacc agcgatcaat
     2641 gccggtagag ttgtccattg tattattggt gagctatgtt gctttacatt aatgacagtg
     2701 atgtgtg
//
LOCUS       DAPHDX010000259         2628 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPHDX010000259 DAPHDX010000000
VERSION     DAPHDX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.263"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..1356
                     /gene="ugd"
                     /locus_tag="RH058_004462"
     CDS             190..1356
                     /gene="ugd"
                     /locus_tag="RH058_004462"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV3139923.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRGADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVSVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            1522..1779
                     /locus_tag="RH058_004463"
                     /pseudo
     CDS             1522..1779
                     /locus_tag="RH058_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1847..2608
                     /locus_tag="RH058_004464"
                     /pseudo
     CDS             1847..2608
                     /locus_tag="RH058_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
BASE COUNT          810 a          567 c          569 g          682 t
ORIGIN      
        1 ccaattatct tatatactaa atttcactgc ttaatattaa cttaataaat atcagctatt
       61 cttataaaga aattctgaat tgtttttcgt tgcgttgacc atcgaacaac gtagcgttaa
      121 aacttttatc tcttatcagg atgctaaaaa catcatgatt cacagttaag ttaattctga
      181 gagcatgaaa tgaaaatcac catttccggt actggttatg taggcttgtc aaacgggctt
      241 ctaatcgcac aaaatcatga ggttgtggca ttagatattt taccgtcacg cgttgctatg
      301 ctgaatgatc ggatatctcc tattgttgat aaggaaattc agcagttttt gcaatcagat
      361 aaaatacact ttaatgccac attagataaa aatgaagcct accggggggc tgattatgtc
      421 atcatcgcca ctccaaccga ctatgatcct aaaactaatt atttcaatac atccagtgta
      481 gaatcagtaa ttaaagacgt agttgagata aatccttatg cggttatggt catcaaatca
      541 acggtttccg ttggttttac cgcagcgatg cataagaaat atcgtactga aaatattata
      601 ttctcacccg aatttctccg tgaaggtaaa gccctttacg ataaccttca tccgtcacgt
      661 attgtcatcg gtgagcgttc agaacgcgca gaacgtttcg ctgcgttatt acaggaaggg
      721 gcaattaagc aaaatatccc aaccctgttt accgactcta ctgaagcaga agcgattaaa
      781 ctatttgcaa atacctacct ggcgatgcgc gtggcgtact ttaacgaatt ggatagctat
      841 gcagaaagtt taggtctgaa tactcgccag attatcgaag gcgtttgtct cgatccgcgt
      901 attggcaacc attacaacaa cccgtcgttt ggctatggtg gttattgtct gccgaaagat
      961 accaagcagt tactggcgaa ctaccagtct gtgccgaata atctcatttc ggcaattgtc
     1021 gatgctaacc gcactcgtaa agactttatt gccgatgcca ttttgtcacg caaaccgcaa
     1081 gtggtgggta tttatcgtct gattatgaag agcggttcag ataacttccg tgcgtcttcc
     1141 attcagggga ttatgaaacg tatcaaagcg aaaggcgttg aagtgatcat ttacgagccg
     1201 gtgatgaaag aagactcatt cttcaactct cgcctggaac gtgatctcgc caccttcaaa
     1261 caacaagccg acgtcattat ctccaaccgt atggcagaag agcttaagga tgtggcagac
     1321 aaagtctaca cacgagatct ctttggcagc gactaatctt ctctgatcag ggcggtttcg
     1381 ccctgattgt cttttgtaac ctttgcaata attcattgtt tttatcattt atcctatatg
     1441 aagtggcact gaagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct
     1501 cagaacaaca caggtgctcc aatgaaaaaa agaaatttca gcgcagagtt taaacgcgaa
     1561 tccgctcaac tggttgttga ccagaactac acggtggcag atgccgccaa agctatggat
     1621 gttggccttt ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa
     1681 acaccaaaag cctctccgat aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag
     1741 ctacaacgca ttgaaatgca gaatgaaata ttaaaaaagg ctactgtaga ttcaattggt
     1801 caacgcaaca gttatgtgaa aacatggggt tgcggaggtt ttttgaatga gacgaacatt
     1861 tacagcagag gaaaaagcct ctgtttttga actatggaag aacggaacag gcttcagtga
     1921 aatagcgaat atcctgggtt caaaacccgg aacgatcttc actatgttaa gggatactgg
     1981 cggcataaaa ccccatgagc gtaagcgggc tgtagctcac ctgacactgt ctgagcgcga
     2041 ggagatacga gctggtttgt cagccaaaat gagcattcgt gcgatagcta ctgcgctgaa
     2101 tcgcagtcct tcgacgatct cacgtgaagt tcagcgtaat cggggcagac gctattacaa
     2161 agctgttgat gctaataacc gagccaacag aatggcgaaa aggccaaaac cgtgcttact
     2221 ggatcaaaat ttaccattgc gaaagcttgt tctggaaaag ctggagatga aatggtctcc
     2281 agagcaaata tcaggatggt taaggcgaac aaaaccacgt caaaaaacgc tgcgaatatc
     2341 acctgagaca atttataaaa cgctgtactt tcgtagccgt gaagcgctac accacctgaa
     2401 tatacagcat ctgcgacggt cgcatagcct tcgccatggc aggcgtcata cccgcaaagg
     2461 cgaaagaggt acgattaaca tagtgaacgg aacaccaatt cacgaacgtt cccgaaatat
     2521 cgataacaga cgctctctgg ggcattggga gggcgattta gtctcaggta caaaaaactc
     2581 tcatatagcc acacttgtag accgaaaata acgttatacg atcatcct
//
LOCUS       DAPHDX010000260         2586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPHDX010000260 DAPHDX010000000
VERSION     DAPHDX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.264"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            274..708
                     /locus_tag="RH058_004465"
     CDS             274..708
                     /locus_tag="RH058_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3139924.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            705..1055
                     /gene="tnpB"
                     /locus_tag="RH058_004466"
     CDS             705..1055
                     /gene="tnpB"
                     /locus_tag="RH058_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3139925.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            1086..2586
                     /locus_tag="RH058_004467"
                     /pseudo
     CDS             1086..2586
                     /locus_tag="RH058_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
BASE COUNT          657 a          661 c          696 g          572 t
ORIGIN      
        1 tgatcacgca gcgttcagtg ataaccagtg aatagcgact ctaatacccg gtcgctctgc
       61 aatgtatcag cgcctgcgac cgatgctatg gatattgatt tttctaccga taaagaacca
      121 gcaatacgga atttagactg gccccctgaa tctccagaca accaatatca cttaaataag
      181 tgatagtcgt aagcgtaaag ccaatgccgt ctgtaacacc tgctccttgc agactaaatt
      241 agagctcctt ctaaattaga cggagttcta ttgatggata aacctacaga ctggcgctcc
      301 ggaacccgcc ggatattttc taatgaattt aaacttcata tggttgaact ggcttcgaaa
      361 ccaaatgcca atgttgcaca actggcccgg gaacatggcg ttgataacaa cctgattttt
      421 aaatggctac gcctctggca aagagaagga cgtatttctc gtagaatgcc tccaactatt
      481 gtaggcccta cagtatcaca atcttttccg gcctctccga ctctggttcc agtggaactt
      541 atcgacaccc cgcgctgtgc tacagatgct cctgctccgg aggcattatc agttgcttgt
      601 gcagcttcct gccatgtgga attccattac ggtaaaatga tgctggaaaa tccttcacca
      661 gagctgctca cggtgttgat ccgtgagctg accgggaggg gacgatgatt tcactcccat
      721 caggtacccg tatctggctc gttgccggcg ttactgatat gcgtaaatcc ttcaacggtc
      781 tgggggagca gatacagcat gtgctggatg ataacccctt ctccggtcac ctgtttatct
      841 tccgtggccg acggggagac acgattaaaa tcctgtgggc tgatgctgat ggtctgtgcc
      901 tgttcaccaa acgccttgag gaaggtcagt ttatctggcc tgcggtgcgt gacggtaaga
      961 tatccattac ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg cgtcagccaa
     1021 aaacatcccg ccttaacgca ctgacaatgt tgtaaaaaac tcctgaccgc attataaaaa
     1081 cggccatgag tcagaaatac ctcattcgca tcgctgagct ggaaaggctg ctctctgagc
     1141 aggctgaagc cctccgtcag aaagaccagc aactgagtct ggttgaagag acggaggcct
     1201 tcctgcgctc tgcactggca cgtgccgaag aaaagatcga agaagatgaa cgggaaatag
     1261 agcatctgcg ggctcagata gaaaaactgc gccggatgct gttcggaacc cgttctgaaa
     1321 aactgcgtcg tgaggttgaa caggctgaag ccctgctgaa acaacgtgaa caggacagcg
     1381 atcgttacag tgggcgggaa gacgatcctc aggttccccg ccagttgcga cagtcgcgcc
     1441 atcgtcgtcc gttaccggca caccttcccc gtgaaatata ccgcctggag cctgaagaaa
     1501 gctgttgccc ggagtgtggc ggtgagcttg attatctggg ggaagtcagc gcagaacaac
     1561 tggaactggt gagcagcgcc ctgaaagtga tccgcacaga acgggtaaaa aaagcctgta
     1621 caaaatgtga ctgcatcgtt gaagcaccgg caccatcccg tccgatagag cgtggtatcg
     1681 cgggcccggg gttacttgcc cgcgtgttaa cgggaaaata ctgcgaacac ctgccactgt
     1741 atcgtcagag tgaaatcttt gcccgccagg gtgtcgaact gagccgtgcc ttactctcca
     1801 actgggttga tgcgtgctgc cagttaatga cgccgctgaa tgatgccctg tacagttatg
     1861 tgatgaacac ccgcaaggtt cacactgatg acacaccagt aaaagtactg gcaccgggca
     1921 ggaagaaggc gaaaacagga tatatctgga cgtatgtccg ggatgaccga aatgccggtt
     1981 cgccagaacc tccggcggtc tggttcgcct actcaccgga ccatcagggt aaacatccgg
     2041 agcaacacct tcgtcccttc cgggtatcct gcaggcagat gcgttcgcag gttacgatcg
     2101 gctgttcagt gccgaacgtg aaggcggcgc gttgacggaa gcaggatgct gggctcatgc
     2161 gcggcgcaaa atccacgatg tatatatcag taccaaaagc gcgacggcgg aagaagcact
     2221 gaaactaatc ggcgaactgt acgccattga gcacgaaata cgcgggttgc cggtgtctga
     2281 acgcctggcg gtcaggcaaa tgcagagtaa accgctactg acttccctgt ataagctgat
     2341 gcaggagaaa gaacacacgt tatcgaaaaa atgccgtctg agagatgcgt tccggtatat
     2401 caggaagcac tgggttgcgt tgtgcaactt ctgtgatgac ggtctggcgg aggcggacaa
     2461 taacacagcg gaaagagcgc ttcgtgcagt ctgtctcgga aagaaaaatt acgtgttctt
     2521 cggtagcgat cacggcggcg agcgtggtgc actgttgtac gggctgatcg gcacctgccg
     2581 gttgaa
//
LOCUS       DAPHDX010000261         2544 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPHDX010000261 DAPHDX010000000
VERSION     DAPHDX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.265"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(50..488)
                     /locus_tag="RH058_004468"
                     /pseudo
     CDS             complement(50..488)
                     /locus_tag="RH058_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            492..662
                     /locus_tag="RH058_004469"
                     /pseudo
     CDS             492..662
                     /locus_tag="RH058_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(739..1567)
                     /locus_tag="RH058_004470"
                     /pseudo
     CDS             complement(739..1567)
                     /locus_tag="RH058_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(1773..2003)
                     /locus_tag="RH058_004471"
     CDS             complement(1773..2003)
                     /locus_tag="RH058_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3139926.1"
                     /translation="MQRHGSIETRTARDKAAERQMSHMDVTQRRPRIIHKAEYLDERK
                     LMLQWWVDFLDANRERAITPFDYAKINRGNGE"
     gene            2235..>2544
                     /locus_tag="RH058_004472"
     CDS             2235..>2544
                     /locus_tag="RH058_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139927.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSKLIYERKSFVIENVSDVSET
                     VKRVEHEIEKRKLSCRVYTEYRSTALAGSLWSPTVILGVASAVAIGVHNLSTWNPDYE
                     I"
BASE COUNT          669 a          621 c          551 g          703 t
ORIGIN      
        1 ggagagattt atattttaaa cgatatattt gtattacata atggattttc taccatattt
       61 ccagacatct gttatcactt aacccattac tgttccaccg ccgcagacac tcctgtggcg
      121 agtggtaaca ccaaagcact atgcagatac cattagttat agtgtccgaa tgcctccgca
      181 agttttttgc tgccgttaac ccgtccggtt tgggcatgat actgatgtaa tcatttcacg
      241 aagctctctg ctattccgtt actctccgga ctccgcaccg ccgtgttctt cggttcaagt
      301 cccaacatcc gggcgaactg gcgtgtttca ttagcccggt agcatgaacc attatccgtt
      361 agccactcca ctggatacgc cggaagctcg ctgccaaagc ggcgttccac ggctcttggc
      421 atgacgtcct gtattgtttc actgtcgaag cagcccgtag tgaccgccca gtgtagtgcc
      481 tcacgctcgc tgaagcgttt tttcttggcg gtgtagagac aacaaaagtg ctgaaggcgt
      541 ttaggccaca cattttcttt gatgataagg atgttgattt gatcgaggct gcaaaggtag
      601 taccttctgg taaagtacct tatgccagta actccgaact ggcagaacgt gaagcatcgt
      661 gattatcact aacaaaccat ttagtcatta aggctggatg gtttgtatac cacacgcaaa
      721 aaactgccct accccagatc aatgttctcc tctgaacacc tcttttctct gccattccac
      781 aaactgttcg cccggataat tttctttaac cggtggcaac gcaatcgctt ttcccgcaaa
      841 atcccagaac agtcgctata ccactccacc accatttacc gcaccggaaa ccaccacacg
      901 catattttca tccaggccaa tcgaaccgcg atcaaacact ttatgatgga ttgcgcacaa
      961 tgccaggcca ttaggtattt cacaagggcc atggtgctgc ttccatctga tatgagcaac
     1021 ttccagagca atcggcgcat tgtcgtggcg catgttaaag ccgcagatag cgcactgata
     1081 attgtaagca cgtagaacca tcttgcgaaa ccccggatcg cgttgacgga gagaagtgcg
     1141 aatatcaaaa tccatctcgt cagcgatctc ttcctgaata ctggccggga aattttgtca
     1201 ccaacgcaaa attatcggca tcaaatccac cagcgacatt atattcaatg agttcacgtc
     1261 ggggcggctg gcggctacca ctggttgagc acagttccgc gttatgtggt tcccagaagc
     1321 catcgccctt cagacgccag aacggcatgt ccgggcgctg gtcacggcgc tgcggtccat
     1381 aacgttccag gagatccaac aggggctcgt agatttcaga agcatagtta aacaggcgac
     1441 catgaccctg ccgatagtgt gagagaacgt acagcagcaa taatggctta tgtggcgcac
     1501 gttgttcgcc tttgcgccag atggtgatat ttgatatcgc ttgctttagg gttttgtcag
     1561 aggtcattgc gctggagaat ccatgagcac atgtttgcga tgatgctcgc aactatatgt
     1621 acattcaagg cattattgca gacatccttt ttgcctgacg gctctgtacc tggcagatgg
     1681 aattctgaaa aaaatacagc gaccaaccag tcctaagcgg tctggttgat cttttaaatt
     1741 taaacatcag ctaaggagta acggcattca actcactcgc cattaccccg attgatcttc
     1801 gcatagtcaa acggcgtaat cgctctttcc cgattggcat ccagaaaatc gacccaccac
     1861 tgcaacatca gcttacgctc atccagatat tccgctttat ggattatacg cggcctgcgc
     1921 tgagttacgt ccatgtggct catctgacgt tcagccgctt tatctcgtgc tgttctggtc
     1981 tcaatacttc catgccgttg caattacctc ttaaaccatt ccttaccatt ttcatgtctt
     2041 cgataagaaa atttcatcat gcatgatcga ttttgtcgat cgattcgatc gaattttctc
     2101 tttttaccct gccttttgca tggttttaaa ataggcaaaa ttacttgtca ttcgtggaaa
     2161 atgatggcgg agataactga gcttaaaaag tttctcttgt ctccgtcact tgccatacca
     2221 gccaggaaaa gaagatgtca gcacaagaaa aaacagtaca gattgattct tttgatgcga
     2281 aatttttaga tataaataca ttaagcaagc ttatctatga acgtaagagt tttgtgatag
     2341 aaaatgtttc cgatgttagt gaaacggtaa aaagagtgga gcatgaaata gagaaaagga
     2401 aattaagttg tcgtgtatat actgagtaca ggagcacagc tttagctggt tctttatggt
     2461 caccaaccgt aattctggga gtggcgagtg cagtagcaat aggtgttcat aatttatcta
     2521 catggaaccc cgattatgaa atcg
//
LOCUS       DAPHDX010000262         2542 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPHDX010000262 DAPHDX010000000
VERSION     DAPHDX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.266"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            185..313
                     /locus_tag="RH058_004473"
                     /pseudo
     CDS             185..313
                     /locus_tag="RH058_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
     gene            311..2080
                     /gene="tssI"
                     /locus_tag="RH058_004474"
                     /pseudo
     CDS             311..2080
                     /gene="tssI"
                     /locus_tag="RH058_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            2156..>2542
                     /locus_tag="RH058_004475"
     CDS             2156..>2542
                     /locus_tag="RH058_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139928.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEP"
BASE COUNT          651 a          629 c          758 g          504 t
ORIGIN      
        1 gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta aattctagct
       61 cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc
      121 ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa caaggagttt
      181 acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa atattactgc
      241 aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg aagacgagat
      301 gctggtgcag gaagaaaacg gtgtgaccga gtggagtccg ctgttcagcg agccgcatcc
      361 ttcccgtgag ttttgtgtcc agtacggcga aaccgattac gatttcctgt gccggatggc
      421 ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa aaaagtaccg accagagcct
      481 ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt gagatcccct ggaatccgaa
      541 cacccgtacc gaggtgagca ccctctgcat cagccagttc cgctacagcg cacaaatccg
      601 tccttcttcc gtggtgacaa aagactacac ctttaaacgt cccggctggg cagggcgttt
      661 tgaccaggag ggccagcacc aggactacca gcgcacgcag tatgaagtgt atgactaccc
      721 cggacgtttc aagggcgccc acgggcagaa ctttgcccgc tggcagatgg acggctggcg
      781 aaacaacgca gaagtggcgc gcggaacaag ccgttcgccg gagatatggc cgggacggcg
      841 aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg gaatggcagg tggtggcgag
      901 cgatcttcac ggcgaacagc cgcaggcgat accgggacgc aggggttcag gcaccacgct
      961 ggataaccat tttgcggtaa taccggcaga cagaacatgg cgaccacagc cgttgctgaa
     1021 accgctggtg gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt
     1081 ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg gaccgttata acccgtcaaa
     1141 ccaggacagt tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gattcggtaa
     1201 cctggcgata ccgcgtgtgg gtcaggaggt gattgtggac ttcctcaacg gcgatccgga
     1261 ccagccgatc attatggggc gcacctacca ccaggaaaac cgcacacccg gcagcctgcc
     1321 gggaacaaag acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga
     1381 actgaagttt gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat
     1441 gaacaccgag gtgctgaata accgcaccac tgatgtgata aacaaccatg ccgaaaaaat
     1501 aggtaacaac caggcgatca ccgttaccaa taaccagatc cagaacattg gcgttaatca
     1561 gatacagacg gttggtgtca accaggtgga aacggtgggc agtaaccaga ttatcaaagt
     1621 gggatcaaac caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtagctta
     1681 ccagacgacg gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt
     1741 agggctgcat aaatcactga tggtgggaat gggctacagc gtcaatgtgg ggaataacgt
     1801 caccttctcg gtgggcaaga cgatgaagga aaacaccgga caaacagcag tttattctgc
     1861 cggtgagcat cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag
     1921 catatttctc aacggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc
     1981 gccagtgatt aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa
     2041 agatttaccc cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaattaaa
     2101 acggaactgg tgcagtgacc agatgaacca tattaaaagc agaaggaatt gatttatggg
     2161 cggcggaaaa ccggcggcgc ggcagggtga catgacccgc aagggactgg atattgtgca
     2221 gggttcagca ggggtgctga taggtgcgcc aaccggtgtg gcctgctcgg tgtgtcccac
     2281 aaaaaaggac agtccgaact atggcagccc ggtgaacccg ctactcggtg cgaaggttct
     2341 gcctgttgaa accgacctgg cactgcccgg cccgctgccg tttattcttt tccgcgccta
     2401 cagcagctac cggaccagga cgcccgcgcc agtgggcgtg tttggtcccg gctggaaagc
     2461 gcccttcgat atccgcttac agatacgcga tgaaggcctg atactcaacg acagcggtgg
     2521 taggagcatt cactttgagc cg
//
LOCUS       DAPHDX010000263         2516 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPHDX010000263 DAPHDX010000000
VERSION     DAPHDX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.267"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            168..656
                     /gene="eco"
                     /locus_tag="RH058_004476"
     CDS             168..656
                     /gene="eco"
                     /locus_tag="RH058_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV3139929.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(805..2451)
                     /gene="mqo"
                     /locus_tag="RH058_004477"
     CDS             complement(805..2451)
                     /gene="mqo"
                     /locus_tag="RH058_004477"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV3139930.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNCDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          601 a          686 c          623 g          606 t
ORIGIN      
        1 tttctgctgg tttgctccct caccagaatt taacactaaa agagcaggta aaattgtctg
       61 aatgttcttt aagttattca taaagcaaat taataaatct gatgaatatg ttaaccttca
      121 gcgacatcat cggtgaaaac ctataaatga agaaggaaag caaaaaaatg aagaccattc
      181 tacctgcagt attgtttgcc gctttcgcta ccacttccgc ctgggcggca gaaagcgtcc
      241 agccactgga aaaaatcgcg ccttatccac aagctgaaaa agggatgaag cgtcaggtga
      301 ttcagttaac cccgcaagaa gatgaatcta ccctgaaagt agaactgtta atcggtcaga
      361 cgctggaagt cgattgcaat ttgcatcgtc tcggcgggaa gctggaaagc aaaacgctgg
      421 aaggctgggg ctatgattat tatgtctttg ataaagttag ctcgccggtt tccacgatga
      481 tggcctgccc ggatggcaag aaagagaaga aatttgtcac cgcgtatctg ggcgatgctg
      541 gaatgctgcg ttacaacagc aagctgccga tcgtggtgta tacgccagac aatgtagatg
      601 tgaagtaccg cgtctggaag gcggaagaga aaattgacaa cgcggtagtt cgctaaaatg
      661 ctgtgaagtg cggcaccccg taggccgaat aaggcggtca cgccgcatcc gacatctaac
      721 gcccgagccg gtgcctgatg cgacgctggc gcgtcttatc agcatacgcc acatccggca
      781 taccatgccg gatgtggcac atcattacaa cgcaatatcc gccacttctt tttgaacggg
      841 ttgcggtttc aactgcggtt ttggcgtact atctgctgcc tgcggcttgt cgtatttcag
      901 ccccagcact tcgctggtgt actgcaactc gcgttctgtt gccgctacat cacagttcag
      961 cttgcgtcca taagacggaa cgatcgcttt caacgtagcc tgccattgcg ggctggaaac
     1021 gcgatcgcca aatacttttt ccagcagatc caacataatc ggcgcggcgg ttgacgcccc
     1081 tggcgatgcc cccagcagcg cggcaatggt cccttgctgg tcactgacca cttcagtacc
     1141 cagacgcagt acgccacctt tctcggcatc acgcttgata atctgcacgc gctgccccgc
     1201 ttgccacaaa cgccagtcct ctttttttgc ctgcggatag tactctttca acgcttcaaa
     1261 acgatcctct tcactcaaca tcacctgact caccagatat ttcaccagat cgaaattatc
     1321 cagcccgacg tgcatcatcg gcatcacgtt agaggtggtg gtggaactca tcagatccca
     1381 caatgaaccg tttttgagga atttggttga gaaggtggca aatggcccaa acagcactac
     1441 gcgtttaccg tccagaacgc gggtatcgat atgcggaacc gacatcggtg gtgcgccaac
     1501 ggatgcttta ccgtaaacct tcgccagatg gtgattaacc acgtccgggt tttccgaaac
     1561 aaggaactgt ccgcccaccg ggaaaccggc gtagtctttc gcttccggaa tccccgattc
     1621 ctgtaatagc ttcagcgccg caccgcccgc gccgataaag acaaatttgg cgcggatgtt
     1681 ctgtgcagtg ccgtttttca gatcggcaac ggtaacggtc caggtgttat cgtcattacg
     1741 ctttagggcg cggacttcgc tgctgagttg cagcgagaag ttagatttct tctgcaagga
     1801 agcaattaac tggcgggtga tctcgccgta gttcacatcg gtaccaattt ccgtacgcgt
     1861 ggctgccact ttctgttgcg gatcgcgccc ttccatcact aacggtgccc actctttgat
     1921 ctgcgcgtga tcttcagagt aacgcatacc gcgaaacagc gagctttgtt gcaacgcggc
     1981 gtaacgggcg cgcaggaaat taacgttatc ctcgccccag acaaagctca tatgcggaac
     2041 ggtattgata aatgaacgcg gagtacgcag cacgccgcgc tcaacctggt gcgcccagaa
     2101 ctggcgggaa atctgaaatg cttcgttaat ggcgactgct ttttcaatac tgatgctgcc
     2161 atcggcgttt tgcggggtgt agttcagttc catcagtgca gaatgcccgg ttccggcgtt
     2221 attccagccg ttcgaactct cctgcgcgac accctccagg cgctccacca tggtcatcga
     2281 ccattcaggc tccagctcgc gtaaataggt ccccaacgtg gcgctcataa tgccgccgcc
     2341 aatcaacagt acatcagttt cctgctcctc ggacgctttc gcttttgccg ccatcgaaac
     2401 ggcattaagc cccacggcca tcgagaagag catggcagtc acttttttca tcttgttaat
     2461 gccttacttt tagtcgcttt attgcaggtg agatttgcgc ggcatcaacg gtaaca
//
LOCUS       DAPHDX010000264         2497 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPHDX010000264 DAPHDX010000000
VERSION     DAPHDX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.268"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            344..487
                     /locus_tag="RH058_004478"
     CDS             344..487
                     /locus_tag="RH058_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV3139931.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            500..709
                     /gene="cspE"
                     /locus_tag="RH058_004479"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             500..709
                     /gene="cspE"
                     /locus_tag="RH058_004479"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV3139932.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            875..1684
                     /gene="rlmA"
                     /locus_tag="RH058_004480"
     CDS             875..1684
                     /gene="rlmA"
                     /locus_tag="RH058_004480"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV3139933.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1681..2247)
                     /gene="mntP"
                     /locus_tag="RH058_004481"
     CDS             complement(1681..2247)
                     /gene="mntP"
                     /locus_tag="RH058_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV3139934.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          662 a          596 c          607 g          632 t
ORIGIN      
        1 ccgttaactg actgttttat gagaaaaagt gatataactt tttattcatt gcatagcaaa
       61 aaatgtgata ttgcacgcac tatgtaataa cttctcccac tggcctggaa caactgaact
      121 tattgaacta tgttagaaaa tacgccagtt taagtatctg cctgaactgg caaggttaag
      181 cacaatgata tatcggcgcg tattccgttg cataagtgtg caaaaaaagt ggaagacgta
      241 tcgagatttg tgcgtctgat cgagacatgt ttaaaaatgg cttgccataa ttaacgttgt
      301 atgtgataac agatttcggg ttaaacgagg tacagttctg tttatgtgtg gcattttcag
      361 taaagaagtc ctgagtaaac acgttgacgt tgaataccgc ttctctgccg agccttatat
      421 tggtgcctca tgcagtaatg tgtcagtttt atctatgtta tgcctgcggg cgaagaaaac
      481 aatctaagga atttttcaaa tggcaaagat taaaggtcag gttaagtggt tcaacgagtc
      541 taaaggtttt ggcttcatta ctccggctga tggcagcaaa gatgtgttcg tacacttctc
      601 cgctatccag ggtaatggct tcaaaactct ggctgaaggt cagaacgttg agttcgaaat
      661 tcaggacggc cagaaaggtc cggcagctgt taacgtaaca gctatctgat cgaatccact
      721 gatctgaagt gtgaatacgc ttcaatctcg ctataaagcc tcgtcgaatg cgaggctttt
      781 tactatgctt tatcttcgct cctggcgttc ggatatttgc ccgccgcgtg attcgcgtta
      841 cacttgcggc ctttagtatc ctgccggagt tgtcatgtcg ttttcctgtc cactttgcca
      901 tcagcctctt tcgcgtgaaa aaaacagcta tatctgtccc cagcgacatc agtttgatat
      961 ggcgaaagaa gggtatgtca atctgctgcc cgttcagcat aaacggtctc gtgatccggg
     1021 cgacagcgcg gaaatgatac aagcacgccg cgcattctta gatgccggac attatcagcc
     1081 gctgcgtgat gcaattgtcg cccaactgcg ggaacggctt gatgaaaagg ccacggcggt
     1141 gctggatatt ggctgtggtg aagggtatta cacacacgca tttgccgatg tgttgcccga
     1201 aatcaccacg tttggcctgg atgtctcgaa ggtagcgata aaagcggcgg cgaaacgcta
     1261 tccgcaggtc actctttgtg tcgcttccag ccaccgtttg ccgttttccg ataccagtat
     1321 ggacgccata atacgtattt acgcgccgtg taaagcagaa gaattagcac gagtagtgaa
     1381 gcccggcggc tgggtcatta ctgccacgcc gggaccgcga catttgatgg agctgaaggg
     1441 gctgatttac aatgaagtac atcttcatgc acctcatgca gaacaactgg aaggttttac
     1501 attacagcag agtgatgagt tgtgttatcc gatgcgtctt cgcggtgatg aagccgtcgc
     1561 attattgcag atgacgccgt ttgcctggcg tgcgaagcca gaagtctggc aaacactggc
     1621 agcaaaagaa gtgttcgact gccagacgga ctttaatatt cacctctggc agcgttctta
     1681 ttaaccgtgg aagtgcgtcc agaggatctg gacgccgatg ccgatcagca ccagcccgcc
     1741 gagaatttcc gcttttttcc caataattga gccgataaag cgaccaacca tcatccctaa
     1801 tgtcgacata atcaaggttg cacaaccaat ggccaatgcg gtcgcgataa tgttgacctg
     1861 caggaaagca agaccaacac ccacagccat ggcatccagg ctggtggcaa tcgcggtggt
     1921 taccagtagc cagaaaccgt gccgacggcg cggctcttca tcttcatcat ctgcgccacg
     1981 aaaaccctca ataatcattc gcccgccgag gaatatcagc agcacaaacg caatccagtg
     2041 gttccattca aggacaaacc ggctggctaa catgcccatt ccccagccga tcagcggcgt
     2101 cagggtttcg acggcaccaa aaataaggcc ggttcgcaat gcttcagaaa atttcggttt
     2161 atggagggtg gcacctttac cgattgatgc agcaaatgca tccatcgaca taccaaacgc
     2221 aagaagaaca gtagcagtga tattcatgac aatgtcctga ccggggtatc cataaaacac
     2281 atcatcgccc ccagcaaaca tgcatacatg aacaacaagc cgtgatggtt ttcgcgttca
     2341 tgcaggcata gcgattgatg tgcatatggt ctcgcctgac tgaaagtatt cagtccgtac
     2401 gtgccacgtt ttgcaacgag tatgttgaca cgtacatttc cggaatctgg aaatcggcta
     2461 ctccccaaat gagggcgcaa ccttaacata ttttgag
//
LOCUS       DAPHDX010000265         2490 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPHDX010000265 DAPHDX010000000
VERSION     DAPHDX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.269"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1045)
                     /locus_tag="RH058_004482"
     CDS             complement(<1..1045)
                     /locus_tag="RH058_004482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139935.1"
                     /translation="MNYAIDKFTGTLILAARATKYAQYVCPVCKKGVNLRKGKVIPPY
                     FAHLPGHGTSDCENFVPGNSIIVETIKTISKRYMDLRLLIPVGSNSREWSLELVLPTC
                     NLCRAKITLDVGGRSQTLDMRSMVKSRQIGAELSVKSYRIVSYSGEPDPKFVTEVERE
                     CPGLPSEGAAVFTALGRGASKGFPRAQELRCTETFAFLWRHPVAPDFPDELEIKSLAS
                     KQGWNLALVTIPEVPSVESISWLKSFTYLPVVPARTSITAIWPFLNQKTSINHVECVY
                     SDTILLSTNMAPTSSENVGPTMYAQGSSLLLSAVGVETSPAFFILNPGENDFVGVSGS
                     IEQDVNLFFSFYKK"
     gene            complement(1067..2281)
                     /locus_tag="RH058_004483"
     CDS             complement(1067..2281)
                     /locus_tag="RH058_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HDV3139936.1"
                     /translation="MHKYPSIIVNINLREAKLKKKVREHLQSLGFTRSDSGALQAPGN
                     TKDVIRALHSSQRAERIFANQKFITLRAAKLIKFFASGNEVIPDKISPVLERVKSGTW
                     QGDLFRLAALTWSVPVSSGFGRRLRYLVWDESNGKLIGLIAIGDPVFNLAVRDNLIGW
                     DTHARSSRLVNLMDAYVLGALPPYNALLGGKLIACLLRSRDLYDDFAKVYGDTVGVIS
                     QKKKQARLLAITTTSSMGRSSVYNRLKLDGIQYLKSIGYTGGWGHFHIPDSLFIELRD
                     YLRDMDHAYADHYMFGNGPNWRLRTTKAALNALGFRDNLMKHGIQREVFISQLAENAT
                     SILQTGKGEPDLTSLLSAKEIAECAMARWMVPRSIRNPEYRLWKARDLFDFISNDSLN
                     FPPFDEIAKTVV"
BASE COUNT          746 a          549 c          462 g          733 t
ORIGIN      
        1 tttttttata gaaagaaaaa aataagttta cgtcctgctc aattgagcca gaaacgccca
       61 caaagtcatt ttctccagga tttagaatga agaaagcagg tgatgtttca acaccaaccg
      121 ctgaaagtaa taaagaggaa ccttgtgcgt acatagttgg tccaacattt tctgatgatg
      181 ttggtgccat atttgttgac aacaatattg tgtcagaata aacacattcg acatgattaa
      241 tacttgtttt ttgatttagg aacggccaaa ttgctgtaat agatgttctg gcaggaacaa
      301 caggaaggta tgtaaaagat tttagccatg aaatactctc cacagaaggg acttcaggaa
      361 ttgtaacaag agctaaattc catccctgtt tactagctaa actttttatt tctaattcat
      421 caggaaaatc tggagcaaca gggtgtcgcc aaagaaaggc aaatgtttca gtacatctta
      481 actcttgtgc tcgtggaaat cccttcgatg ccccacgccc taaagcagtg aaaactgctg
      541 ctccctcaga aggtaaacct gggcattctc tttcaacttc tgttacaaat tttggatctg
      601 gttcaccact atatgaaaca atacggtaag attttactga taattcagca ccaatctggc
      661 gactctttac catactcctc atatcaagcg tttggcttct gcctcctaca tctaacgtta
      721 tctttgctct acataaatta caggttggca acactaattc taatgaccac tctcgactat
      781 tacttccgac aggaatcaat aagcgcaaat ccatatatcg ctttgaaata gttttaatag
      841 tttcgacaat gatagaattt ccgggaacaa aattttcaca gtctgacgta ccatgtccgg
      901 gcaaatgagc aaaatatggg ggtataacct tccctttacg gaggttaaca ccttttttac
      961 aaactgggca aacatattga gcatatttcg ttgctcgagc tgctaatatc agtgtcccgg
     1021 tgaacttatc aatagcgtaa ttcacttact cccccttcag ttaagattag acaactgttt
     1081 tcgctatctc gtcaaacggg ggaaagttta gcgagtcatt actaataaaa tcaaatagat
     1141 ctcttgcttt ccaaagccga tattctggat tgcgaattga tcgtggaacc atccatcgtg
     1201 ccatcgcaca ctcagctatc tctttagcag aaagcaaaga ggttagatct ggttcacctt
     1261 tgcctgtttg cagaatacta gttgcatttt ctgctagctg actgataaac acttcacgtt
     1321 gaattccatg cttcatcaaa ttatctctaa atcctagtgc atttaaagct gccttagttg
     1381 tacgtaaacg ccagttaggc ccattaccaa acatataatg atctgcataa gcgtgatcca
     1441 tatcacgtaa gtaatcacgt aattcaatga acaagctatc aggtatatga aaatgccccc
     1501 aaccgcctgt atatccaatc gattttaaat attgaattcc atccagcttt aaacggttat
     1561 ataccgatga gcgccccata gacgatgttg ttgtaatagc caaaagacgt gcttgtttct
     1621 ttttttgaga tattactcca acggtatcac catagacctt tgcaaagtca tcataaagat
     1681 cgcggctacg aagcagacat gcaattaatt ttcctcccag cagggcatta taagggggaa
     1741 gagcaccgag gacgtatgca tccatcaaat taacaagccg ggaacttctg gcatgagtat
     1801 cccacccaat caaattatct cggactgcaa ggttgaacac agggtcacca attgcgatca
     1861 gccctatcaa ttttccgttg ctttcatccc atacaagata ccggagacgc cttccaaatc
     1921 cgcttgaaac aggtacggac caagttaatg ctgctaacct aaagagatct ccttgccagg
     1981 ttcctgactt tacacgttca agtaccggtg aaatcttatc cggaatgacc tcattgccgg
     2041 atgcgaaaaa tttaataagc tttgccgctc ttagcgttat gaacttttgg tttgcaaata
     2101 tccgctcagc tcgttgagaa ctatgaagag cccgtattac atctttggta tttcccgggg
     2161 cctggagcgc tccagaatca gatcttgtaa aacccaagga ttgtaaatgc tcacgtacct
     2221 tctttttcag tttggcttct cgaaggttga tattaactat tatagaggga tatttgtgca
     2281 tttttatctc acgttccaat tagtatttag tcccactaaa tttcacttag agccccagtg
     2341 attaatcgca tggcacaaga gagtcccctc cttgctccct cgatcgggac agcgccaatg
     2401 atagcattta tacaagcaat tctgggagct tatagattgt gtgcaaaact tttgatagca
     2461 ggagcttaca acagtattga tgaaaaaaat
//
LOCUS       DAPHDX010000266         2484 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPHDX010000266 DAPHDX010000000
VERSION     DAPHDX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.270"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..137
                     /locus_tag="RH058_004484"
     CDS             <1..137
                     /locus_tag="RH058_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDV3139937.1"
                     /translation="SHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASVWKYVI
                     "
     gene            complement(552..752)
                     /locus_tag="RH058_004485"
     CDS             complement(552..752)
                     /locus_tag="RH058_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV3139938.1"
                     /translation="MVIRKKKCRDCGNAITHNTVYCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            952..1521
                     /locus_tag="RH058_004486"
     CDS             952..1521
                     /locus_tag="RH058_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3139939.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
     gene            1688..2089
                     /locus_tag="RH058_004487"
     CDS             1688..2089
                     /locus_tag="RH058_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139940.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREVRLIKRLQQFVPDALPVVRKANWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            2077..>2484
                     /locus_tag="RH058_004488"
     CDS             2077..>2484
                     /locus_tag="RH058_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV3139941.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDILCCVSCGCPLTLKR
                     GNHGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDAR"
BASE COUNT          615 a          614 c          676 g          579 t
ORIGIN      
        1 tttcccacac gcgttataaa cttacctctg cggaactgga agccttcaac tctgtggttg
       61 atcactggct ggcagaactg acaatgaaca agctttacga tcgtgttccc gcttcagtct
      121 ggaaatatgt catctgagcg ttcggtaatc acaactgcat cgcaccaggt gttatccgct
      181 ttgtgattct tattggctct tctcaggtta ttaaaagctg aatatgttcc tgttgtcggg
      241 aaccaggata ataccgggaa cgttaagcgg tatagcgaca acaaagaatt cgaaatcaaa
      301 agggaatggt gccggaggcg tgggcggtaa tgggctgatt ttcaggtatt accggcttac
      361 agcgtgtggt tgatattgag gggctgtgag tttcacgtat ttgtcagaaa cccagacgag
      421 ttttcctgta tgggacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg
      481 aaggagaacc tgtcggttac gcttaatata accggtacac tgtcagctca ggggatacat
      541 gaaccgttat ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg
      601 accacaataa tcagtgccag cagtatggta acgatacggt ctgtattccg gtaatagccg
      661 aaggggtcga cagaaccaca atacgggcaa tacacagtat tatgagtaat cgcgtttcca
      721 cagtccctgc acttcttttt gcgtatcacc ataacgccct cctcacagcc ttacccgtaa
      781 ccggtttttt acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgaccg
      841 ttcgggttat ctcagagtgc ctgagacagt gtcctgccgg aggtcacttc agggactctg
      901 cgcatttttt acgacgtggc tattccgtat gaaccatacg gagatttaac catgacctac
      961 aaatacaacc ccttctggca gcaacgtatt cgtgagacgg tgcggcacgc actgaatgtt
     1021 catccccgcc tgacggcatt gcgggttgac ctgcgtttcc cggatgtacc ggcagcaacg
     1081 gacgcagctg tgatatcccg cttcatcaat gccctgaaag cccgaatcga cgcttaccag
     1141 aaacgtaagc atcgggaagg taaacgcgtg catcccacca ccctgcatta cgtctgggcc
     1201 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggttaaccgg
     1261 gatacctggt gtcgtgccgg tgattaccgt gctccgggat cactggccgg gatgattaaa
     1321 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
     1381 ccggcctggc cggcggtgtg gctggaacgc gatgatgaca ctggctttca gcaggtgctg
     1441 gaacgtgctg actatctggc gaaggaacat accaaagctc actgcaccgg tgagcgcaac
     1501 tttggctgca gtcgtggctg agcccgacag acgacttatc tgaccttagt gtgatgacgc
     1561 gttcacagcg accacactga ttttcccttc tgagactgaa ctgaaaaccg ctattcacac
     1621 ctgaccacgt tgcccgtacc gggacggctg ttgcctgcgt gtcgtctggc ggctgaggaa
     1681 tatgactatg tacgcaaaat cctttatcgc tcttgatggc aacggacgtc tgacgggcgc
     1741 ccgtactgca caggccgcac cttatgctaa ctacacctgc cacttgtgtg gcagtgcact
     1801 cagataccat ccgcaatacg acactgaacg tccctggttt gaacacactg acgacgggct
     1861 gacagcgcac ggtcagcagt gtccttatgt cagaccggaa cgcagggagg tacgcttgat
     1921 taagcgtctg cagcaattcg taccggatgc cttacccgtg gtgcgtaaag ccaactggca
     1981 ctgcagacaa tgtcaccacg attattatgg ggagcagtac tgcacaaact gccagaccgg
     2041 aggattcagt attccccgga caactcagga ggaaatatgc gaattctgaa ctgttatatg
     2101 gcgaatgaca gcaaaggcca ttttgttacg gcgaaagaag ctgcgaagca caaccgacag
     2161 gacattttgt gctgtgtgtc ctgtggatgc cctttaacac ttaagcgggg caatcacgga
     2221 caaccaccgt ggtttgaaca tgaccagatg actgtcgctg agaaaatcct gctgcgatgc
     2281 acctggcttg acccggcaga gaaagaggcc cgtcgtttgc atctgcaggg tatgacggtt
     2341 ccggattata cggtgaaggt gagaaagtgg ttttgtgtga tgtgtgacga agattatgaa
     2401 ggagaaaagt gctgcccgcg ttgtggtacc ggggtataca gcagggcgtg ggggcggcag
     2461 gaggtgccgt cggaagatgc cagg
//
LOCUS       DAPHDX010000267         2380 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPHDX010000267 DAPHDX010000000
VERSION     DAPHDX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.271"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..867)
                     /locus_tag="RH058_004489"
     CDS             complement(<1..867)
                     /locus_tag="RH058_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDV3139942.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTK"
     gene            complement(1540..1740)
                     /locus_tag="RH058_004490"
                     /pseudo
     CDS             complement(1540..1740)
                     /locus_tag="RH058_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1740..2339
                     /locus_tag="RH058_004491"
                     /pseudo
     CDS             1740..2339
                     /locus_tag="RH058_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          512 a          701 c          631 g          536 t
ORIGIN      
        1 tttggtgcct ttgacgatga ggacaaaatc atcggcatag cggcagtacg ccaccgcggg
       61 tttccactgc cagttttctc tgaccgccgt acttcggccc cgttggatac tgttattcca
      121 gtaccaccga tcttttctgg ctttcccgct caggtagcgc tcatgcaggt attgatcgaa
      181 ctcattcagc atgatgttcg ataatagcgg cgatataaca ccgccctgtg gcacaccttc
      241 actggccgcc cgaaagagac cgacatcgat atgtcccgcc ttgatggttt tccacagcag
      301 agtcatgaaa cgtgcgtcac tgatcctgcg gcgtacagcc ttcatcagca gtcgatgatg
      361 tacggtgtcg aagtaactgg acaggtcgcc ttcaatcacc cagcgtcccc gggtttcacc
      421 acagtctgtg agctgtaatt tcaccgtgcg gatagcgtgg tggacactgc gctcaggccg
      481 gaagccatat gagagcgtat gaaaatcact ctcccatatc ggctccatcg ccatcagcat
      541 ggcccgctga acaatacgat cccgcaacgc ggggataccc agtggtcgca gtttgccgtt
      601 gcttttaggg atgtaaaccc gtctggcggg caagggctgg tagtggcctg agagtaattc
      661 atccctgagg atttgcagct caacagccag tctggcctgt agcattgttt tgttcacgcc
      721 atcaacgccg ggggtatggg ccccctttga tgaaagcgtg atccgcgccg cttcagccag
      781 ccattctggt tgtgttatca gacgcagcag ccgttgaatc cgtagggacg gatcggtgac
      841 tgcccatgtg gcaagcttgc gttgcatttc gctgattatc aaaggtcttc acctcgttag
      901 gtcagttaat tcacgtcgca aacacattca aactgcttcc cttcgccatg taatgggctt
      961 tccccatcgc ggactactac ggaagctccg ccagccagcg cgtcatcgga gccatgcccc
     1021 cttaacatcc gtcgctgacc ttccccggtt tacctgcctg gactcaggca tactgaggag
     1081 gctgcccgtc gcactcttta tccttgcttg ccgcaagttg gcagaagtca gcaacgcaag
     1141 cgtgatagac gctgctgccc cagtgtttcg catacatgtc aaaacacctt cgaccggcag
     1201 tgcttacgta tcactgccag ttcctcctgc acggcctgtc agatcacgta ggccgtggtg
     1261 acgttttcaa cccacagagg cggattaacg ggttcatgtt cttcagcctt tcagtactta
     1321 accttgagga tcatctcggc ttagtgatct cgcctcaatc cccgttgtca gcgggttaca
     1381 tcaccctgcg ggcatgccgc aggtcactgc cgctcaggtt ctccaccgtc acacccggtg
     1441 ggattgttgg gtttctcatc gtgagttacc ggttcaatat tccagacaga ctcgcggttc
     1501 atttaagcat ccatgcccgc cctgaactcc gggcacaccg taagtaaaat cagccaccca
     1561 caactggtca ggacgttctg ccacaaactg acggtttacg cggtcgcctg cggcaacggc
     1621 tttccggctg acggtagtgc ggaccttttt accccggaga acatcggcaa gtcccataac
     1681 cgccatgagg cgcgccactg tacatctggc taccctgatt ccttcccgta acaactgacg
     1741 ccgggttcaa cgagctgtac cgggatggcc ggataacgga agccgcctgt tgggctcacg
     1801 cccgccgtaa aatccacgat gtgcacgttc gcaccccgtc agccctgacg gaggaagcgc
     1861 tgaaacggat cggcgaactg tacgccatcg aggcagagat aaggggaatg acggcggagc
     1921 agcgccttgc cgaacgtcag ttgaaaacga aaccgctgct gaaatccctg gaaagctggc
     1981 tgcgtgaaaa gatgaaaacc ctgtcgcgac actcagaact ggcgaaagcg ttcgcatacg
     2041 ccctgaacca gtggccggcg ctgacgtact atgcagatga tggctgggct gaggcggaca
     2101 ataacatcgc tgaaaatgcg ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct
     2161 tcggttcgga tcatggagga gagcggggag cgctgctgta cagcctgatc gggacgtgca
     2221 aactgaatgg agtggagcca gaaagctacc tccgctatgt ccttgacgtc atagccgact
     2281 ggccgataaa ccgggtcggc gaactgctcc cctggcgcgt agcactgccg actgaataac
     2341 acatccccgt caatacggtt cttgctgcac gcttacaatt
//
LOCUS       DAPHDX010000268         2371 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPHDX010000268 DAPHDX010000000
VERSION     DAPHDX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.272"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..813
                     /locus_tag="RH058_004492"
     CDS             <1..813
                     /locus_tag="RH058_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3139943.1"
                     /translation="LSRHILEHLRKLTRHEPVIGIMGKSGAGKSSLCNALFQGEVTPV
                     SDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLI
                     KADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIRE
                     KTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESV
                     RSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            1185..>2371
                     /gene="ag43"
                     /locus_tag="RH058_004493"
     CDS             1185..>2371
                     /gene="ag43"
                     /locus_tag="RH058_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3139944.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADSIVQAGETVNGGTLENHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAV"
BASE COUNT          552 a          620 c          703 g          496 t
ORIGIN      
        1 ctttcccgtc acatcctgga acatctgcgc aaactcaccc gtcacgaacc cgtgattggc
       61 attatgggta aaagcggggc cggtaaatcc tcactctgta atgcactgtt tcagggggag
      121 gtcaccccgg tcagtgatgt tcacgccggc acccgggaag tgcagcgctt ccgtctgagt
      181 ggccacggtc acagcatggt tatcactgac ctgcccgggg tgggcgagag ccgggacagg
      241 gatgcagagt atgaggccct gtaccgtgac attctgcctg aacttgacct ggtactgtgg
      301 ctgattaaag ccgatgaccg tgccctgtct gtggatgagt atttctggcg acacatcctg
      361 caccggggac atcagcgggt actgtttgtg gtgacgcagg ccgacaaaac ggagccctgc
      421 catgaatggg atatggccgg tattcagcct tctcctgcac aggcacagaa tattcgcgaa
      481 aaaacggagg cggtattccg tctgttccgg cccgtacatc cggttgtggc cgtatcggcc
      541 cgcaccggct gggaactgga tacgctggtc agtgcgctca tgacagcgct tcccgaccat
      601 gccgccagtc ccctgatgac ccgactgcag gacgagctgc gcacggaatc tgtccggtct
      661 caggcccgtg aacagtttac cggtgcggtg gaccggatat ttgacacggc ggagagcgtc
      721 tgtattgcct ctgttgcacg cacggttctg cgtgccgtcc gtgactcggt ggtctctgtt
      781 gcccgcgctg tatggaactg gattttcttc tgaacactct gccaggggaa atgcctgatt
      841 atattgcatt aacaaactga aaacatgaca caaacccctc ataccctcct gtttctcttg
      901 cgtgaccgcc ttgctgttaa tagaataaaa cgatcgataa aacaggtatc gatcgctgct
      961 atcgatcgtt tatagtggtt aatggcctgc gaaaaggcag ctgaatctct tcatcatgca
     1021 gaacggaatt gcacacaacg gactgatact tctctggcgg atgagagagg ggaagcattt
     1081 atgtccggga aaaaccactt tatctgacca tgctgttttg tacctgccgg tatccacatt
     1141 tgtgggtacc ggctttttta ttcaccctct gtaaggaaaa gctgatgaaa cgacatctga
     1201 acaccagcta caggctggta tggaatcaca ttacgggcac cctggtggtg gcctccgaac
     1261 tggcccgctc acggggaaaa ggcgccggtg tggcggttgc tctgtctctt gctgctgtca
     1321 cgtcagtccc ggccctggct gctgacagta ttgtacaggc aggtgaaacc gtgaacggcg
     1381 gaacactgga aaatcatgac aaccagattg tcctcggtac ggccaacgga atgaccatca
     1441 gtaccgggct ggaactgggg ccggacagtg aagaaaacac cggtgggcaa tggatacaga
     1501 atggcgggat agccggaaac accactgtta ccacaaatgg tcgtcaggtc gtgctggagg
     1561 ggggaacagc cagtgatacg gttattcgtg acggcggggg acagagcctg aacgggctgg
     1621 cggtgaacac cacgctgaat aacagaggcg agcagtgggt tcatgagggc ggggttgcca
     1681 ccggtacaat tatcaaccgc gacggttacc agagcgttaa aagtggcggg ctggcaacag
     1741 gaaccatcat caacaccggc gcagaaggcg gcccggattc tgacaactcg tacacgggtc
     1801 agaaggtcca gggaacagca gaatccacca ccatcaacaa aaatggacgg cagattatct
     1861 tattttccgg gatagcccgt gacactctca tttacgcagg tggtgaccag tcggtacacg
     1921 gaagggccct gaataccaca ctgaatggcg gttaccagta tgtgcacaag gacggacttg
     1981 cgctgaacac ggtaattaac gaggggggct ggcaggttgt taaggcaggt ggtgctgtcg
     2041 gtaacaccac cataaatcag aacggtgaac tgagggtaca tgccggcggg gaagccactg
     2101 cagtcaccca gaacacgggc ggtgcactgg ttaccagtac ggctgcaacc gttaccggca
     2161 taaaccgcct tggagcattc tcggttgtgg agggtaaagc tgataatgtc gtactggaaa
     2221 atggcggacg cctggatgtg ctgaccggac acacagccac taatacccgc gtggatgatg
     2281 gcggaacgct ggatgtccgc aacggtggca ccgccaccac cgtatccatg ggaaatggcg
     2341 gtgtactgct ggccgattcc ggtgccgctg t
//
LOCUS       DAPHDX010000269         2368 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPHDX010000269 DAPHDX010000000
VERSION     DAPHDX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.273"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..745
                     /locus_tag="RH058_004494"
     CDS             176..745
                     /locus_tag="RH058_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3139945.1"
                     /translation="MTYKYNPLWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLVHFPVRPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            912..1238
                     /locus_tag="RH058_004495"
     CDS             912..1238
                     /locus_tag="RH058_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139946.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSTLRYHPQY
                     DTELPWFEHTDEGLTKHGHECPYVRPECREIQLIKRLQKFVPDALPVVRKASWHCTKQ
                     GIGSCR"
     gene            1204..1488
                     /locus_tag="RH058_004496"
                     /pseudo
     CDS             1204..1488
                     /locus_tag="RH058_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1868
                     /locus_tag="RH058_004497"
     CDS             1494..1868
                     /locus_tag="RH058_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139947.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEAMGILNKYPLAKGRVTWSE
                     IRHSDYESFDELLSANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
     gene            2015..2368
                     /gene="tnpA"
                     /locus_tag="RH058_004498"
                     /pseudo
     CDS             2015..2368
                     /gene="tnpA"
                     /locus_tag="RH058_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          634 a          528 c          636 g          570 t
ORIGIN      
        1 ccggcttttt gcattaaaaa aacaacgctg aaaattcagc cgtcagtccg gagacgacag
       61 tcgggttatc tcagagtgcc tgagatattg cctggttgga gatcacttca gggactctgc
      121 gcatttttta cggcgtggtt aatccgtatg aacgaaacca tacggagatt taaccatgac
      181 ctacaaatac aaccccctct ggcagcaacg tattcgtgag acggtgcggc acgcactgga
      241 tgttcatccc cgtctgacgg cattgcgggt tgatctgcgt ctcccggatg taccggcagc
      301 aacggacgcg gctgtgatat cccgcttcat caatgccctg aaagcccgca tcgacgctta
      361 ccagaaacgt aagcatcggg aaggtaaacg cgtgcattcc tcgaccctgc attacgcctg
      421 ggcccgggag ttcggggagc ttaaaggcaa aaaacactat cacctgctgc tgctggtcaa
      481 ccgggatacc tggtgtcgtg ccggcgatta ccgcgctccg gggtcactgg ccggaatgat
      541 taaacaggcc tggtgcagtg ccctgggggt ggatgccgga tgttatgcca cgctggtgca
      601 ttttccggtc aggccggcgg tgtggctgga acgcgatgat gaaaccggct ttcagcaggt
      661 gctggaacgt gctggctatc tggcgaagga gcataccaaa gctcgcggca ccggtgagcg
      721 caactttggc tgcagtcgtg gctgagcccg acagacgact tatctgacct tagtgtgatg
      781 acgcgttcac agcgaccaca ctgattttcc cttctgagac tgaactgaaa accgctattc
      841 acacctgacc acgctgcccg taccgggacg gctgttgcct gcgtgtcgtc tggcggctga
      901 ggaatatgac gatgtacgca aaatcctttc tcgcacttga tggcaacggg cgtctgactg
      961 gtgcccgtac cgcacagact gcaccttacg atcgctacac ttgccacctt tgcggcagta
     1021 cactcagata ccatccgcaa tacgacactg aacttccctg gtttgaacac actgacgaag
     1081 ggctgactaa gcacggtcat gagtgccctt atgtcagacc ggagtgcaga gaaatacaac
     1141 tgattaagcg tctgcagaaa ttcgtaccgg atgccttacc cgtggtgcgt aaagccagct
     1201 ggcactgcac gaaacaaggc attggatctt gtaggtaatt taggagcgga ctctaagcca
     1261 gttatcggac gtttggaagt cagcgctggt aatgggaaag tcatagggcg gcaatccagc
     1321 gacggaaaag ttggttggcg tgttgattat gatcctgaaa aaggcacaca catcaatata
     1381 tgggattact cacagggtaa aggtcctgga aaagcggtga agcaagtcat accttttgaa
     1441 ggcaatgaaa agtcttttga aacaaaattg aaacagctaa acaggtagag taaatgactt
     1501 tatttgatga gtgtcgggaa gcactgagtg ctgattttaa tatagtagag gggctggctc
     1561 agcaggaggc tatgggcatt ttaaataagt atcctctcgc gaaaggacgt gttacttggt
     1621 cagagatcag gcattctgat tatgagagtt ttgatgagtt attgagtgca aactctgtaa
     1681 aaaacgatga catgtttgtc tttgcagatg atgcaagtat tccagttttt aggtcaaatc
     1741 taagattaat cgctgaaaat atctatgacg taacagcttt atcgcccaaa ttattcattt
     1801 ttaatgatga agtgataata caacctttat tcccaactga tatgttccgt ttaggaatca
     1861 agaaatagtg ggaaagctcg caaatcccgg ctaggctggg ggttccggaa agctttcagc
     1921 tttgagccag ttattaaaac cccttttgat ttgttaaaac accttgcggt ctggcaactg
     1981 caagtgtcaa acatgaaatc aaaagggggt cccaatgggg aacgaaaaga gtttagcgca
     2041 cacccgatgg aactgtaaat atcacatagt ttttgcgcca aaataccgaa gactggtgtt
     2101 ctacagagag aagcgtagag caacaggcag tattttgaga aagctgtgtg agtggaaaag
     2161 cgtacggatt cttgaagctg aatgctgtgc agatcatgtc catatgattg tggagacccg
     2221 cccaaaatga gcgtatccgg ctttatggga tatctgaaag ggaaaagcag tccgatgctt
     2281 tacgagcagt ctggggattt gaaattcaaa tacaggaaca gggagttctg gtgcagaggg
     2341 tactacgtcg atacggtggg taagaaca
//
LOCUS       DAPHDX010000270         2343 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPHDX010000270 DAPHDX010000000
VERSION     DAPHDX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.274"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            494..721
                     /locus_tag="RH058_004499"
     CDS             494..721
                     /locus_tag="RH058_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139948.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(756..896)
                     /gene="ykgO"
                     /locus_tag="RH058_004500"
     CDS             complement(756..896)
                     /gene="ykgO"
                     /locus_tag="RH058_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV3139949.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(896..1159)
                     /locus_tag="RH058_004501"
     CDS             complement(896..1159)
                     /locus_tag="RH058_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV3139950.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            complement(1522..1623)
                     /locus_tag="RH058_004502"
     CDS             complement(1522..1623)
                     /locus_tag="RH058_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139951.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
BASE COUNT          682 a          435 c          523 g          703 t
ORIGIN      
        1 tcttacttgc attttatatt ggtcaggaac tttgccctga agtgttagga atagtcttaa
       61 tgcgctgtgt gatggactag taaaaaaaca agttttaagg gtaagggata gaatataaaa
      121 ctataatgag ctgtcattga agatgatttg ccaatcagta agaaatttta atggattaac
      181 atgccgcaca aaatggttat aggtgttcat tacaataagt gatggaaaat tgaacgggcg
      241 tatgtacgtc atctgttcgg taagccccca agagccagat taagtgaagt taaacgaagg
      301 ctgccagctg tcacaggaac gatggcctct ttgtcagggc actgagtgca gttatctgtg
      361 tgcgttactc taactggcac tcgaacgtcg gcgacggggt taccggtgga tcagactgtt
      421 cctgtggcgt aaacgttttt atgttagtac caacagattt accacattta tcatcttaac
      481 agtttgagtg aaaatgcagg ctgcgccgca aagggaggag actgagtggc gtgttcagtc
      541 taaaagagag ttgatgccag catacagggg cgaagctggt caacaagtca acattaagat
      601 aatggaatat tcggagagaa acgtcaggca gttggcctca aatgaacagg aggaatatat
      661 ccccaggaaa attaatgttg gtgttataaa cacaccaaca ttaatccgat ctgattatta
      721 atcagacaaa tatcgttaat ccgtcgaatt ttgaatcaac gttttttctt acgaccctga
      781 acggccttaa aacgtggatt agatttacaa atcacatata gccgtccttt tcgcttcaca
      841 atctgacagt ctggatggcg ttcttttgcg gtacgcagag agttaagaac tttcatcacg
      901 accccttttt cgtgctaaca aaacgaccaa aacgttgggt gaatcgtgca acatttcctt
      961 ctgatgccac tgttctcagc ttccctgtat agaacgggtg cgatttagaa gagacatcaa
     1021 ttgtcacgta tggatacgtt acgccatcca gctcaatctc acggtctgtt ttgatagtcg
     1081 agccgatttt aaagtactca tcaacactgg tgtcgtggaa caccacagta cgatactcag
     1141 gatggatatt gggcttcatc atttttacct gttatgttat aacataacca taaagataca
     1201 tgctcatccg gatcatttca acacccaatc cctgtgactc tttgtggatg tggaattttc
     1261 gcaacgggga actctggcat tgttagcagc cattggtgac gctcccataa acgcctggat
     1321 gagtgattga tgatggtttt gctgagagta tggatttttc gacaatggag gttatctcca
     1381 ctttactggc cgatgattta tcgcagtatg attgattacc tgacagcgtg tattcctctc
     1441 ccacggatat aataattact tacaaaaagg gagaggatgc atattttaaa tatcactgaa
     1501 gtgaacagtt tattgccgtt attaatagaa atggagaaat aaataggcgt attctacaat
     1561 tgcgacaaaa acaacgatat taatcagttt atgactgatt tgttgtactt tattctcttt
     1621 cattggtact tcctcgcttt aaaaaagagc gcacttcgta agtgccctta tataaataac
     1681 gagtttggtc aaccaatttt ttgatatgta tcacaaattt gaatagatgt attacatcgg
     1741 ctatctttta ttgcaccaac gtcattgata tatgtcgcct gaagtcagtt ccgggaatga
     1801 gtctgatctc aagactggcc cagtccgggc gttgattggc gctgaggagc atacctcatt
     1861 tcatcataat gtcgtatctc ctggggtgtt atacaagata tcattgttgg taacctggga
     1921 aaggaattga gttctattaa accgtcaact atgccggata catactggat tacactgcag
     1981 gcacgcctta tgagagaacg tgccgcagtg acgggttaat tatctgaaag aatttgtgag
     2041 gctgtatcgg ttactcattg atttgatagt tttactctcg ggagagtaat agatatttaa
     2101 tctattaacg gaaaccagcc agttcctttc gatgcctgaa tttgattcca tagtttatcc
     2161 ggaatggtta attcatgaat aagttgtgtt ctcgttcatg agaagcataa cgtaaaggga
     2221 aaagctcgat tagacggcag gatttgtcag gggttatgaa cgaaattcat aaatctgttt
     2281 gagtgttgcg atgggtagtg caagttcgaa atctccgcaa tttacagtcc gatgaaggaa
     2341 aat
//
LOCUS       DAPHDX010000271         2324 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPHDX010000271 DAPHDX010000000
VERSION     DAPHDX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.275"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..813
                     /locus_tag="RH058_004503"
     CDS             <1..813
                     /locus_tag="RH058_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3139952.1"
                     /translation="LSRHILEHLRKLTRHEPVIGIMGKSGAGKSSLCNALFQGEVTPV
                     SDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLI
                     KADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIRE
                     KTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESV
                     RSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            1141..>2324
                     /gene="ag43"
                     /locus_tag="RH058_004504"
     CDS             1141..>2324
                     /gene="ag43"
                     /locus_tag="RH058_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3139953.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VMSVPALAADIVVRPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKKGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAV"
BASE COUNT          535 a          616 c          691 g          482 t
ORIGIN      
        1 ctttcccgtc acatcctgga acatctgcgc aaactcaccc gtcacgaacc cgtgattggc
       61 attatgggta aaagcggggc cggtaaatcc tcactctgta atgcactgtt tcagggggag
      121 gtcaccccgg tcagtgatgt tcacgccggc acccgggaag tgcggcgctt ccgtctgagt
      181 ggccacggtc acagcatggt tatcactgac ctgcccgggg tgggcgagag ccgggacagg
      241 gatgcagagt atgaagccct gtaccgtgac attctgcccg aactggacct ggtgctgtgg
      301 ctgattaaag ccgatgaccg cgccctgtct gtggatgagt atttctggcg acacatcctg
      361 caccggggac atcagcaggt gctgtttgtg gtgacgcagg ccgacaaaac ggagccctgc
      421 catgaatggg atatggccgg tattcagcct tctcccgcac aggcacagaa tattcgcgaa
      481 aaaacggagg cggtattccg tctgttccgg cccgtacatc cggttgtggc cgtatcggcc
      541 cgcaccggct gggaactgga tacgctggtc agtgcgctca tgacagcgct tcccgaccat
      601 gccgccagtc ccctgatgac ccgactgcag gacgagctgc gcacggaatc tgtccgctct
      661 caggcccgtg aacagtttac cggtgcggtg gaccggatat ttgacacagc ggagagcgtc
      721 tgtgttgcct ctgttgtccg tacggccctg cgcgctgttc gtgacacggt ggtctctgtt
      781 gcccgtgcgg tatggaactg gattttcttc tgaacctgtc gtgactgatg ccctccctga
      841 ctctgagtct gctcacaaaa gcgctgtttt cgttactgtc tctcttgtcc gtgcaatagc
      901 tcaataatag aataaaacga tcgatatcta ttttatcgat cgtttatatc gatcgatagg
      961 ctaataataa cctttgtcag taacatgcac agatacgtac agaaagacat tcagggaaca
     1021 acagaaccac aattcagaaa ctcccacagc cggacctccg gcactgtaac cctttacctg
     1081 ccggtatcca cgtttgtggg taccggcttt tttattcacc ctcaatctaa ggaaaagctg
     1141 atgaaacgac atctgaacac cagctacagg ctggtatgga atcacattac gggcaccctg
     1201 gtggtggcct ccgaactggc ccgctcacgg ggaaaacgcg ccggtgtggc ggttgctctg
     1261 tctcttgctg ctgtcatgtc agtcccggca ctggctgctg acatcgttgt acgcccggga
     1321 gaaaccgtga acggcggaac actggcaaat catgacaacc agattgtctt cggtacgacc
     1381 aacggaatga ccatcagtac cgggctggag tatgggccag ataacgaggc caataccggc
     1441 gggcaatggg tacaggatgg cggaacagcc aacaaaacga ctgtcaccag tggtggtctt
     1501 cagagagtga accccggtgg aagtgtctca gacacggtta tcagtgccgg aggcggacag
     1561 agccttcagg gacgggcagt gaacaccacg ctgaatggtg gcgaacagtg gatgcatgag
     1621 ggggcgatag ccacaggaac cgtcattaat gataagggct ggcaggtcgt caagcccggt
     1681 acagtggcaa cggataccgt tgttaatacc ggggcggaag ggggaccgga tgcagaaaac
     1741 ggtgataccg ggcagtttgt tcgcggggat gccgtacgca caaccatcaa taaaaacggt
     1801 cgccagattg tgagaactga aggaacggca aataccactg tggtttatgc cggcggcgac
     1861 cagactgtac atggtcacgc actggatacc acgctgaatg ggggatacca gtatgtgcac
     1921 aacggcggta cagcgtctga cactgttgtg aacagtgacg gctggcagat tgtcaaaaaa
     1981 gggggtgtgg ccgggaatac caccgttaat cagaagggca gactgcaggt ggacgccggt
     2041 ggtacagcca cgaatgtcac cctgaagcag ggcggcgcac tggttaccag tacggctgca
     2101 accgttaccg gcataaaccg cctgggagca ttctctgttg tggagggtaa agctgataat
     2161 gtcgtactgg aaaatggcgg acgcctggat gtgctgaccg gacacacagc cactaatacc
     2221 cgcgtggatg atggcggaac gctggatgtc cgcaacggtg gcaccgccac caccgtatcc
     2281 atgggaaatg gcggtgtact gctggccgat tccggtgccg ctgt
//
LOCUS       DAPHDX010000272         2308 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPHDX010000272 DAPHDX010000000
VERSION     DAPHDX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.276"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..719)
                     /locus_tag="RH058_004505"
     CDS             complement(<1..719)
                     /locus_tag="RH058_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3139954.1"
                     /translation="MASENDKNHKVRVAQYLRMSTDHQQYSLHNQSEYIKDYAEKNNM
                     EIAYTYDDAGKSGVSIIGRHSLQQLLSDVEQKKIDIQAVLFYDVSRFGRFQNSDEAAY
                     YSFLFERNGVDLIYCSEPIPTKDFPLESSVILNIKRSSAAYHSRNLSEKVFIGQANLI
                     KLGYHQGGMAGYGLRRLLVDENGIAKEILGFRKRKSIQTDRVILIPGPKNEIKIVNSI
                     YDLFIDDNMPEFIIAERLNEQ"
     gene            complement(1017..1544)
                     /locus_tag="RH058_004506"
                     /pseudo
     CDS             complement(1017..1544)
                     /locus_tag="RH058_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641070.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1602..1781
                     /locus_tag="RH058_004507"
     CDS             1602..1781
                     /locus_tag="RH058_004507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139955.1"
                     /translation="MRKLLSATGAELTNGVTLPSGWLVSGMSKGANNPENLRAFAVCM
                     RQWQHKNSRINFCLN"
     gene            1905..2270
                     /locus_tag="RH058_004508"
     CDS             1905..2270
                     /locus_tag="RH058_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139956.1"
                     /translation="MIKQKLKNDWDIPAILRLTKEQKRLKLSVQEVADLLDLSSYFFE
                     LAVDEKVDFKFYDLPEKVKKKLHYAGFDIFYLMTGEYSGSEPALKIASFEYSIKDMSP
                     EEKNEVRVLIENIPITKPH"
BASE COUNT          694 a          423 c          437 g          754 t
ORIGIN      
        1 ttctgttcat ttagtctctc agcaataatg aattctggca tgttatcatc tataaagaga
       61 tcatagatgc tatttacaat ttttatctca ttttttggtc ccggaattaa tatcacccta
      121 tctgtttgaa tactctttct tttgcggaag cccaatattt ctttagctat gccattttca
      181 tctactaaaa gacgtcttag cccataacca gccattccgc cttgatgata accaagcttt
      241 attaaatttg cttgccctat aaataccttt tctgataaat tcctgctgtg atatgcagca
      301 ctagatcttt ttatattcag tataacagaa gactctaaag ggaaatcttt agtgggaata
      361 ggttcggaac agtatataag atctacaccg tttctctcaa atagaaagga ataatatgca
      421 gcttcatcac tattttgaaa acgaccaaaa cggctcacat cataaaataa tacagcctgt
      481 atatctattt tcttttgttc tacatcacta agtaactgct gcaaagaatg ccggcctatg
      541 atactgactc cgctcttacc tgcatcatcg taggtataag cgatttccat attgttcttt
      601 tcagcataat ctttgatata ttcggactga ttatgtaaag aatattgctg atggtcggta
      661 gacatccgca agtactgtgc gaccctaact ttatgatttt tatcattttc gctagccatg
      721 cacgaaacct catggatgat tcaatacaat gtagatgata aaattgatca atttctaacc
      781 aatttcaact gttttatcat tgattagagc gaatttagta aatcttcgag gtgtatgata
      841 atcgagaaga tatgtgaatt atgtagctga tatgaccaac agctttcggt atgtggcctc
      901 aagttaccca tccgtaaatg gctatgctta gtcaacattt gttaatatgt gtgattataa
      961 ttctttaact aaatcaagat agttaaccca tcaggggaga gttatgcggc aataacttag
     1021 catttttgag acgcaacata gtcactccac cattgcatga gaaccacccg ttctgtggga
     1081 tattctgcac gattgtaggc tgcaattatt tcatctttct tcgagtgggt aagggcggct
     1141 tcgaagacat cagtcctaaa ctttccagac tcatcagcag catcgcatac cgtgagctac
     1201 aagcttacct ccgaacccca tacggattat ggccgcatta gctgtttgct catgcatatg
     1261 gttaagagga gcctttatgc ttggaaacac ccattcacga tgcccactta tcactttcat
     1321 tgactctagg actcgtaaag cttctttgct tagtggaact ttgtgaggct tcttcatttt
     1381 cataaactca gccgggatgt tccacatacc agtttctata tcaatatctg tccatcttgt
     1441 gcgaaccgct tcagcgggcg aacccatgtt agaagttgcc actcaatcag tagccttgtt
     1501 tctaaccgga tagaagcatt gtttagagca actaggaagc ggggggatta ctgtttctgg
     1561 taaagactct tttggtggtg aactggacgc ctggaatcaa tatgagaaaa ctacttagtg
     1621 caacaggagc tgaattaact aatggtgtaa cattacctag tggttggctt gtcagtggta
     1681 tgagcaaggg agccaataat cctgaaaatt taagagcgtt tgctgtttgt atgagacaat
     1741 ggcagcataa aaattctagg ataaactttt gccttaatta aaatgatagg tggtatattt
     1801 ataggggtta tcttaggaag aatattttta atgctattga tgagaagtat cggacatgat
     1861 ttaatgttgt ttatgtttta gaattctcat attcaggcgg tttcatgata aaacagaagt
     1921 taaagaatga ctgggatatt cctgcaattc ttcgtcttac taaagaacaa aaaaggctaa
     1981 aattgtctgt tcaagaagtt gcagaccttc tggacttaag ctcatacttt tttgagttag
     2041 ctgtcgatga gaaggtggat ttcaaatttt atgaccttcc tgaaaaggtt aaaaaaaagc
     2101 tgcattatgc aggttttgat attttctatt taatgactgg tgagtattca ggtagtgaac
     2161 cggcattgaa aatcgccagt tttgaatatt ctataaagga tatgtctcct gaagaaaaaa
     2221 atgaagtgag agtgttgata gaaaatatac cgataacaaa accacactaa aattaccttt
     2281 aatcctatca tgttatattg gtaggaaa
//
LOCUS       DAPHDX010000273         2266 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPHDX010000273 DAPHDX010000000
VERSION     DAPHDX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.277"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            599..868
                     /locus_tag="RH058_004509"
     CDS             599..868
                     /locus_tag="RH058_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139957.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            865..1146
                     /locus_tag="RH058_004510"
     CDS             865..1146
                     /locus_tag="RH058_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3139958.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            1231..1578
                     /locus_tag="RH058_004511"
     CDS             1231..1578
                     /locus_tag="RH058_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139959.1"
                     /translation="MPFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVAVLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1596..2186)
                     /locus_tag="RH058_004512"
     CDS             complement(1596..2186)
                     /locus_tag="RH058_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139960.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVCLEGVPLEVAQWLQACHDAGIEVRVQINESSVP"
BASE COUNT          505 a          627 c          639 g          495 t
ORIGIN      
        1 atttaaaaag aaaacctgtt cactgtctgt cctgtggaca gacagatatg cacctcccac
       61 cgcaagcggc gggccccgac cggagccact ttagttacaa cactcagata caaccaccaa
      121 aaaaaacccg gtcccgcgca gaactgaaac cgcaaagccc cccctcataa ctgaaaagcg
      181 gccccgcccc ggcccaaagg gccggaacag agtcgctttt aattatgaat gttgtaacta
      241 agcatttcca tcgctgtcag tcttctcgct ggaagtatcg agtacacgct cgtaagcggc
      301 cctgacggcc cgctaacgcg gagatacgcc ccgacttcgg gtaaaccctc gtcgggacca
      361 ctccgaccgc gcacagaagc gctatcatgg ctgaaagcgg gtatggctta gcagggcggg
      421 gatgggacag gtgaaatcta tcaatcagta ccggcttacg ccgggcttcg gcggttttac
      481 tccggtatca tatgcaacaa tggagtgccg ccttccatgc cgctgacgcg gcatatcctg
      541 gtgacgatat ctgaatcgtt atatactgtg tatatacgta gtaatgacga ggtgataaat
      601 ggcacaggtt aatatgagtt taagaatcga cgctgaactg aaggatgctt ttatggccgc
      661 tgcaaaaagc atggatcgta atggctctca gttaatccgg gattttatgc gccagaccgt
      721 tgaacggcag cataatacct ggttccgcga ccaggttgcg gcaggacgtc agcaactcga
      781 gcacggcgat gtgcttcccc atgacatggt cgaatcatct gctgcagcat ggagggatga
      841 aatgagcagg aaggtcgccg gtaaatgatg gagatattct ggaccatgct ggccagccag
      901 gacaggaagc gcattcgtga gtacattgcg gagcagaacc tgatggccgc cattgaactg
      961 gacgaacgga ttggttattc agccagcagc ctggccgggc agccgtataa aggccgcaac
     1021 ggtcgtgtgg aaggtacccg ggaactggtt attcacccac attttgttct ggtttatgag
     1081 gtcgacagcc agtggggaaa agtgtatatc ctgcgagtat tgcatactgc acagaagtgg
     1141 ccatagaggg gctgtccgga tatcacagca tgataataat caataacaat aagctgtgtc
     1201 acgtttacat catggaacgg aggtgctcct gtgccctttg agcagaaaaa agcccgggcc
     1261 attgccctga tggacagcaa aaagatgtgg cggagcaatt atgcccctcc tcttctgcgt
     1321 attctctggc ggctgggtat ccggcttccg ccattaccct ttatgccgtt ctggcaggtc
     1381 gcggtgctga cggggggctt atggggcact tcctggggat gtgcaatgtg gtttatctac
     1441 tgggggccgt caggaatggt ggcaggtgag gccattatta taagtatcac gggtggtttt
     1501 ttgttcggac tgctcacggc ctctttccac tggtggcgca ggaaagttaa ccggctgcca
     1561 ccatgggatg acgtgtaaac catgcaggca tgtttttacg ggacgctact ttcatttatc
     1621 tgtacgcgaa cctctattcc tgcgtcatga caggcctgca gccactgcgc cacttccagc
     1681 ggaacgccct ccaggcatac caccctgcct tctttattcc ataactgcag acaggtgctg
     1741 ccgtcgagac gcaccacaaa atccccacgg caggcctgat acgttatgcc gttcagctct
     1801 accgccagat cagtggcaga agtgccgtct gcgggcagac ctgctgccgg gaccagcggc
     1861 agtacgacga cttctgccgc acgctgttct gcccggaggc ggtcctgttc tgccagcagc
     1921 agaggctgtg ccacggcccg gccggcgtcc gtcagctcca ctgccagctg caggttcgga
     1981 gcgcgcaggg tacgaagcca gccggcatcc tccagacgac ggcaggaggc gcgcaggttt
     2041 ggcccgtaga ccggggcttc cccgcttcgc tccagcaccc gttctatgtc ccgcgtcagc
     2101 acgggtcccg gccgtttttc atcaagggca gccagcacaa tcagcactct ccgctgcagt
     2161 ggcgacggtc ggcggctctg agtcattaac tgtcaccctc cgaccagatg agttttttga
     2221 ggcgctgata atcggtatcg atgcgttccc gggcctgtgc gtgatt
//
LOCUS       DAPHDX010000274         2242 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPHDX010000274 DAPHDX010000000
VERSION     DAPHDX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.278"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..462
                     /locus_tag="RH058_004513"
     CDS             <1..462
                     /locus_tag="RH058_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139961.1"
                     /translation="NSIEKDDVYIANCIEKGKTEFKVSKDIAALPFKGEPIEKIRGEL
                     IRSIKEHLISASWHSTKKVIDRYEATFDIKVQKDCPIEAIELATLNRNHLVHRGGKDK
                     EGNLVVITDQDLETLIENASNLAIMLYNSLNVATNKTTILQPDDKPFIHEF"
     gene            478..1479
                     /locus_tag="RH058_004514"
     CDS             478..1479
                     /locus_tag="RH058_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139962.1"
                     /translation="MDIRTRKTKFLESLDSTEVIRKAVSLAIDCIIDNHNSNEDIPLV
                     ITSYDDLCRIQVLNYVQEFCEAAFPDMDEYYFSPNILRINGKTSEEACINLIKLLRST
                     KGMLFWSDAPSWFASLPDGLFHVVNIDQKIVTRGLNKKNSKPTIINKDYSVDTLLSEL
                     FLNGAHMEQPNVHNVSEGNMKFYDECHAGLIRPIPAPIGASYDEEITINSPDWQKLAC
                     VALRRYQSKECHDGMQWDTTDHGWTDVIAYPFVEEIQSMDNSGYRQCLVGLVTINNSN
                     ANSPYLSTVWIHPFYRRRGLLSKLWPKLQELYGSNFEIERPNENMKAFLKSAKYADY"
     gene            2097..>2242
                     /gene="hokD"
                     /locus_tag="RH058_004515"
     CDS             2097..>2242
                     /gene="hokD"
                     /locus_tag="RH058_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV3139963.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRLRTGQTEVAVF
                     TAYEP"
BASE COUNT          716 a          435 c          498 g          593 t
ORIGIN      
        1 aattctatag agaaagatga tgtctacata gccaattgta tagaaaaagg taagactgaa
       61 tttaaagtaa gcaaagatat tgctgcgttg cctttcaagg gtgaacccat cgagaaaatt
      121 aggggagagc taatcaggtc aatcaaggaa cacttgatca gtgcaagctg gcatagcact
      181 aaaaaggtaa tcgaccgcta tgaagctaca ttcgatataa aagtacaaaa agactgtccg
      241 atagaagcta ttgaattggc aaccctcaat cgaaatcact tagtgcatcg tggtggaaag
      301 gataaagaag ggaacctcgt agtgattaca gaccaggatc ttgaaacgct gattgaaaat
      361 gcatcgaacc tggcaattat gctctacaac agtttaaatg tagcaacgaa taaaaccacc
      421 attttgcaac cagatgacaa accgttcatt catgaatttt aatagcgcag gtcatacatg
      481 gatattcgca cacgaaagac aaaattttta gagtcgttag attcaactga agtgatacgc
      541 aaagcagtaa gtctcgctat agattgcatt attgataatc ataatagtaa tgaagatatc
      601 cccttagtta ttaccagcta tgatgatctc tgtagaatcc aggtgctaaa ttatgtacag
      661 gagttttgcg aagctgcatt ccctgatatg gatgaatatt actttagtcc taacatactt
      721 cgcatcaacg gcaagactag tgaagaagct tgtattaact taattaaact cttaagaagt
      781 acaaaaggca tgctattctg gtccgatgcc ccatcgtggt tcgctagtct ccccgacgga
      841 ttattccatg ttgttaacat tgatcaaaaa atcgttactc gcggccttaa taagaaaaac
      901 tctaagccga caataatcaa taaagattac agtgtagata cactactatc agagctattt
      961 ttgaatggcg cacacatgga gcaacccaac gtacacaacg tttctgaggg aaacatgaag
     1021 ttctatgatg aatgccatgc tggattaatc agaccaatac ccgctccaat aggcgcgtca
     1081 tacgatgaag agatcacaat taattctcca gattggcaaa agctcgcgtg tgtagcttta
     1141 cgccgctatc agtcgaagga atgtcatgac ggaatgcaat gggatacaac cgatcatggt
     1201 tggactgatg taatcgcgta tccattcgtc gaagaaatac aatcaatgga taatagtggt
     1261 tatcgacaat gtttagttgg gctggtcaca ataaacaatt caaatgcaaa ttctccgtac
     1321 ctatctaccg tttggataca tccattttat cgcaggagag ggttattaag taagttatgg
     1381 ccaaaattac aagagttata tggaagtaac ttcgaaattg agcgacctaa tgaaaatatg
     1441 aaagcatttc tgaaaagtgc gaaatacgca gattactaat ttatgaatca gtctgtggag
     1501 ttagggttgg tgtcaacaag tagggtaaga gcctcaacgt gatgctatgc cgacctgctc
     1561 cctgagaatt cacacagaag gctgctagca atgtctacag atcgctcaaa gcggactaga
     1621 aggttagctt gcgtcggact tggcgtattt aaagaagtgc tggtggtgac tggttgttgt
     1681 gttccatttc tacagaacaa aatcacagaa actataccca atagttatat tgaatcaatg
     1741 atgagacagc ctcatattta tcaggactgg tgtacgtcca atacaggagg ttgtcgtgct
     1801 ggttctcaaa tgtgcgctag ctattgcggc tgtaatggca atttattgtc ttgctgttgt
     1861 tcttacggat cgcctttctg attgatttta tattggcgag gtgacgtgag ttaagtagaa
     1921 ttgctgcggg tgcttgaggc tatctgcctc aggcatgaac accaaaggca gatagagaaa
     1981 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gccaatttca
     2041 tgctagacac atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga
     2101 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     2161 cactggtaac gaggaaagac ctctgcgagg tacgactccg aaccggccag acggaggtcg
     2221 ctgtcttcac agcttacgaa cc
//
LOCUS       DAPHDX010000275         2233 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPHDX010000275 DAPHDX010000000
VERSION     DAPHDX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.279"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..533
                     /locus_tag="RH058_004516"
     CDS             30..533
                     /locus_tag="RH058_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3139964.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(579..995)
                     /gene="rraB"
                     /locus_tag="RH058_004517"
     CDS             complement(579..995)
                     /gene="rraB"
                     /locus_tag="RH058_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV3139965.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1157..2161
                     /gene="argF"
                     /locus_tag="RH058_004518"
     CDS             1157..2161
                     /gene="argF"
                     /locus_tag="RH058_004518"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV3139966.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRTLAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          567 a          534 c          565 g          567 t
ORIGIN      
        1 taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa
       61 tgcgtcgcct gacgcagcag gataatcccg ccatcgcccg cgtcattcgt caggtatccg
      121 ccgaatacgg tcttaccgct gataaaggct acaccgtcgc cgatccgaac ctggacgagt
      181 tgtatcaagt gtatagtcag ccgggtcatg catattgggt ggttgagtac gatggtgaag
      241 tggtcggcgg cggtgggatt gcgccattaa ccgggagtga gtcggacatt tgcgaactgc
      301 agaagatgta ttttcttccg gctattcgcg gtaaagggct ggcgaaaaaa ctggccttaa
      361 tggcgatgga gcaggcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt
      421 ttttgaaaga agcgattggt ttgtatgaac atttaggttt tcaacatatc gactatgcac
      481 ttggctgcac gggccatgta gattgtgaag tgcggatgct acgcgaactc taaaaaaatg
      541 cccgctagtg tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
      601 tcttcatcga caaaatcttc atcgtcgccg tcttcgccat tgggatcttc aaagtaagtg
      661 ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg
      721 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
      781 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
      841 tcaactgctg ctttttccag ggtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
      901 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgtgtttct
      961 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     1021 gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgacaa agatttatgc
     1081 tttagacttg caaatgaata atcatccata taaattgaat tttaattcat taaggcgtta
     1141 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     1201 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa
     1261 aagcggcaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     1321 ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccagggtg ctcgcgttac
     1381 ttatctcggc ccaagcggca gtcagattgg tcataaagag tcgattaaag acactgcccg
     1441 cgtgctgggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgtcga
     1501 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
     1561 cacgcagttg ctggcggatc tcctcaccat gcaagagcat ttgcccggta aagcgttcaa
     1621 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
     1681 agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccgga
     1741 agctgcgctg gttacggaat gccgcaccct ggcacagcaa aatggcggga atattacgct
     1801 gacggaagat gtagccaaag gagttgaagg tgctgacttt atctataccg atgtatgggt
     1861 gtcgatgggg gaagcaaaag agaaatgggc agaacggatt gcattactgc gtgattatca
     1921 ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct
     1981 gcccgcgttt catgacgacc agacgacgct tggtaagaaa atggcggaag agtttggcct
     2041 acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtttttga
     2101 tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaaata
     2161 attgatatcg ctataaagat gccgggaaat acaaaaacat cccggcatca cattatttta
     2221 tgcagtaata gtt
//
LOCUS       DAPHDX010000276         2174 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPHDX010000276 DAPHDX010000000
VERSION     DAPHDX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.280"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..980)
                     /locus_tag="RH058_004519"
     CDS             complement(<1..980)
                     /locus_tag="RH058_004519"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024418.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3139967.1"
                     /translation="MNKIYNIVWNESSGMWVVTSELTRKGGQRHRKIKRTMLAGLIAG
                     LMLPAIPAMAQMYNDKTLEYSDSAMELSAGDTATNTTINGGEQYISSGGNATSTTINE
                     GVQIVFDGGTASTTTINGGYQEISSGGLATNTIIDGGDQYISSGGNAIDTTIENGYQT
                     VFSGGNATSTIINGGFQEVSSGGSATSAIINAGFQTLYDNSIASSTVINNGFQLISSG
                     GSSVDTTIQGGIQEVGEGGSASATTINDGFQILLSGGSATNTTINNGWQDISSGGSAT
                     QTIISGGIQTIYDGGSASEITINSGYQVISSGGSVTTTTIYRGGEQSITNA"
     gene            complement(973..2166)
                     /locus_tag="RH058_004520"
     CDS             complement(973..2166)
                     /locus_tag="RH058_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HDV3139968.1"
                     /translation="MTPPEKPTQSGPEGIRYDFNDGARVLLPEGKWHVRLMDADSGNI
                     LFSCDADNGWVRSCKKYFIRFRIQVFHRGNDTPLMDETLNLKNQPVLISFPTGTLGDL
                     LGWFPYAERFQTLHKCQLECTMAQEIIELLAPQYPQIQFSTPDNPHTITPYATYRVGL
                     YFGGDTTNQPIDFRQVGFHRSAGYILGVDPRESPVRLNLSAPRSINEPYVCIATQSTC
                     QAKYWNNGTGWSEVVAHLKSLGYRVLCIDLHAHYGQGFVWNHIPWGAEDFTGKIPLQE
                     RVNLLHHASFFIGLSSGLSWLAWATHIPVVLISGFTLPVSEFYTPWRIFSSHGCNGCW
                     DDTSLNFDHKDFLWCPRHKNTPRQFECTRLITGKQVNGVIDKLHASLAEQSSIGMNHS
                     RWSNE"
BASE COUNT          595 a          535 c          443 g          601 t
ORIGIN      
        1 gcattagtaa tactctgctc cccaccacgg taaatggtcg tggtggttac actgccaccg
       61 cttgagatga cctggtaacc actgttgatt gtaatctccg acgctgagcc tccatcgtaa
      121 atcgtctgga tacccccgct aatgatcgtc tgagttgcag agccaccact ggatatatcc
      181 tgccaaccat tattaatagt ggtatttgtg gcacttcctc cactgaggag gatctgaaaa
      241 ccatcattta tagtcgtagc acttgcactt cccccctcac caacttcctg aattccgcct
      301 tgaatagtgg tgtcaacaga actgccacca ctagaaatga gctggaagcc attattaatg
      361 acggtgctac tagcaatact gttatcgtag agagtctgga atccagcatt aataatggca
      421 ctggtagcac tgcctccact ggaaacttcc tggaacccgc cattaataat tgtacttgta
      481 gcgttgcctc cactgaaaac tgtttggtat ccattttcaa tagtggtatc aatagcatta
      541 cctccactgg agatatattg atctccccca tcaatgatcg tattagtggc taggccgcca
      601 cttgagattt cttggtaacc accattgatg gttgtcgttg aggctgtgcc gccatcaaat
      661 actatctgaa ctccttcatt aatagtcgta cttgtagcgt tacctccact ggagatatat
      721 tgttctcccc cattaatggt agtattggtt gccgtatcgc cagcggataa ctccatggcg
      781 gaatcactat actccagagt tttatcatta tacatttgtg ccatagccgg tatggccggc
      841 aacataagtc ccgcaattag tccggctaac attgttctct ttatttttcg atgccgttgc
      901 ccacctttgc gggttagctc agaagtcacc acccacatac cagaagattc gttccataca
      961 atattataga ttttattcat tactccacct tgaatgattc ataccaatac tactttgttc
     1021 tgccagagag gcatgaagtt tgtcgatgac gccgtttacc tgcttccccg taatcaggcg
     1081 tgtacattca aattgtctgg gggtgttttt atgtctcgga caccacagaa aatctttatg
     1141 atcaaaattc agtgaagtat catcccagca tccgttacat ccatgggaac taaatattct
     1201 ccatggggta taaaattcac tgacaggaag cgtaaaacca cttattagta caacagggat
     1261 atgagttgcc catgccagcc aggagagccc gctggataaa ccaataaaaa aactggcatg
     1321 atgtaacaga ttcacccgtt cctgcagagg tattttccct gtaaagtctt ctgctcccca
     1381 cggaatatgg ttccagacaa agccctgacc ataatgagca tgcagatcaa tacacagtac
     1441 ccgatacccc agcgatttga gatgggctac aacctcactc cagccagtac cgttgttcca
     1501 gtatttcgcc tgacaagtgg attgtgtcgc gatacataca taaggctcat taatgcttct
     1561 gggggctgaa agattaagtc ggactggtga ttctcgggga tccactccca gaatgtatcc
     1621 agcggaacga tgaaaaccga cctggcgaaa atcaattggc tggttagttg tatcgccacc
     1681 aaaatacagt cctacacgat aagttgcata tggcgtaatc gtatgaggat tatctggagt
     1741 agaaaattga atctgcggat attgcggtgc cagtagttca ataatctcct gtgccatagt
     1801 gcattccagt tgacacttat gcagcgtctg aaacctttca gcataaggaa accatccgag
     1861 taaatcacct aatgtaccgg taggaaacga aatcagcaca ggctgatttt ttaaattcaa
     1921 cgtttcatcc attaagggcg tgtcgtttcc cctatggaat acctgaatac gaaaacgaat
     1981 gaagtatttt ttacagctcc ttacccagcc attatctgca tcgcagctaa aaagaatgtt
     2041 ccccgaatca gcatccatca gacgaacatg ccatttcccc tcagggagca gtacacgcgc
     2101 cccatcatta aaatcatatc ggataccctc aggtccggac tgagtcggtt tctctggcgg
     2161 agtaataaaa tatg
//
LOCUS       DAPHDX010000277         2173 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPHDX010000277 DAPHDX010000000
VERSION     DAPHDX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.281"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(56..>2173)
                     /locus_tag="RH058_004521"
     CDS             complement(56..>2173)
                     /locus_tag="RH058_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3139969.1"
                     /translation="QRVSSGGSAVGTTINGGLQTVFGGGNVSGTLINNGEQRVSSGGS
                     AIDTTINNGGTMSIAAGGSGTGIIQNNGGAIRANTSAILSGTNVNGSFSITNGSARNM
                     LLENGGLLMVLQGHQAIDTQVSKGGELQVESGSELLGTTLIKDDGKLSGNQITNNGVL
                     HFLDNSSANYSGTLDGTGSLNLNGGKLTLSGNFSQDRGIFIQNHGTMIMDNLRAMANV
                     TIEPDATLRLEDGSLLAGRITGDGKKGGNLIVADSVWKLSGDSTINSLDMSNGTVDFH
                     SIQSPQFKTKLEGVTLNIGQLSGNGTFLMNTDIASYTGDKLNITGDANGNFVLNIKNT
                     GQEPVSPGNPLPVVYTGGGDAVFTVNGGKVDAGVWEYYLSKDNTNWYLKSDTTQPGNP
                     EPGIPNPPVRHTTKSTDAVLNMASAPTYIFNSELQGLRFRNSEKSENSRTSGGVWGHY
                     IGSDKHISSKGAGYSLTQYGIVTGGDKRFDLSDGVLSLGAFVSFSDNNIRHNRGGKSS
                     IDSKSVGLYMTYDTHGYYIDGVLKFNRFKNELRTHMSDGTMVSGDYHQNGFGGSLEVG
                     KYFSLNENTWLLPYARTTALAVNDNNISLSNGMKAEINTTHSFQGEVGVNLGMDLDVG
                     KTKIRPYLTAGVSHEFSNHNKVKINKAYDFTNDISGTTGKYGLGMDVQLTSNASIWGE
                     ANYLKGNNVESPVTGTVGFKINF"
BASE COUNT          571 a          542 c          392 g          668 t
ORIGIN      
        1 attagtaatt cagatgccca accgatatgg tcgggcatct gatgcacatc aggcgttaaa
       61 agtttatctt gaagccaaca gtccctgtta ccggtgactc tacattgtta ccttttaaat
      121 aattagcctc tccccatata ctggcatttg atgttagctg aacatccata cccaaaccgt
      181 actttccggt tgtcccggat atgtcgttag taaaatcata ggctttattg attttaacct
      241 tattatgatt tgaaaattca tgactgactc ctgcggtcag gtagggcctt attttggttt
      301 taccaacatc aaggtccatc cctaaattaa caccaacctc accctggaaa gaatgtgttg
      361 tgtttatttc agccttcatt ccattagata gactgatatt gttatcattc acagcaagag
      421 ctgttgttcg ggcataagga agtaaccagg tattctcatt cagagaaaaa tacttgccca
      481 cctccaggct accaccgaaa ccattttgat gataatctcc cgaaaccata gttccatcac
      541 tcatatgggt acgcaactca tttttgaaac ggttgaattt gagtacacca tctatatagt
      601 atccgtgagt atcatacgtc atgtacaagc caacactttt actatcaata gaacttttcc
      661 ctcctcgatt atgcctgata ttgttatcag agaaggaaac aaatgcccct aatgacaaca
      721 ccccatcgct taggtcaaaa cgcttatctc cgcccgtcac tattccgtat tgggtcaggg
      781 agtaacccgc tcctttgctg ctgatgtgtt tatctgatcc tatatagtgc ccccatactc
      841 ccccagaagt acgtgagttt tctgattttt cactgttacg gaatcgcagc ccttgtaatt
      901 cactgttaaa gatatatgtc ggagcgcttg ccatattcag tacagcatca gtagactttg
      961 tggtgtgccg tactggagga ttgggtatac ccggttccgg attacctggt tgtgtagtat
     1021 cagattttag ataccagtta gtattatctt tactcagata atattcccaa actcctgcat
     1081 ccacttttcc tccgtttact gtaaatacag catctcctcc accggtataa accactggta
     1141 atgggttacc cggggataca ggctcctgac cggtattttt gatattcaaa acaaaattac
     1201 cgtttgcatc ccctgtgatg tttaatttat caccagtata tgatgcaata tcagtattca
     1261 tcaagaaggt gccatttcct gataactgtc cgatatttaa cgttactccc tcaagttttg
     1321 ttttaaactg aggggactgt attgaatgaa aatccacggt tccattactc atgtctaatg
     1381 agttaatagt agagtctccg gataatttcc agacagagtc agccactatc agatttccac
     1441 ctttcttccc atccccagta atgcgtccag ccaggaggct accatcctct aagcgcagag
     1501 ttgcatcagg ctcgattgtc acatttgcca tagcccgtag attatccatt atcattgttc
     1561 catgattttg gataaaaata cctctatcct gagaaaaatt accactgaga gttaattttc
     1621 ccccattcag attcagacta ccggtaccat ccagtgtgcc gctatagttt gctgaactat
     1681 tgtcgagaaa atgcagaacc ccattattgg ttatctggtt gcctgacagt ttgccatcgt
     1741 ctttaatcaa ggttgtaccc aatagctcag aaccgctctc aacctgtaat tcaccacctt
     1801 tagatacctg ggtatctata gcctgatgac cctgcagaac catcaggagt ccaccatttt
     1861 ctaacaacat atttctggcg ctaccattag taatgctgaa gctaccattt acgttcgttc
     1921 cactcaggat ggccgatgtg ttcgctctga ttgcgccacc attattttga attattcccg
     1981 ttccactacc acctgcggca atactcattg tccctccatt gttaatggtt gtgtcaatag
     2041 cactgccacc actggagact cgctgctccc cgttattaat gagagtccct gagacatttc
     2101 cgccaccgaa aacagtctgc agtcccccat taatggtggt gcctacagca ctaccaccac
     2161 tggagactcg ctg
//
LOCUS       DAPHDX010000278         2146 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPHDX010000278 DAPHDX010000000
VERSION     DAPHDX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.282"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            259..915
                     /gene="tssI"
                     /locus_tag="RH058_004522"
                     /pseudo
     CDS             259..915
                     /gene="tssI"
                     /locus_tag="RH058_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            911..2146
                     /locus_tag="RH058_004523"
                     /pseudo
     CDS             911..2146
                     /locus_tag="RH058_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          495 a          622 c          621 g          408 t
ORIGIN      
        1 aggtttttta ttactcgctg taatgtacga gcctgtcgtg aataacgtcg agaacccatc
       61 cgttgcgttt gtgattattt tgttgactaa acagacaccc gtttctctga agtaaaatcc
      121 cagactaaat catcacataa ccatgacatt tttcttatat tccccggtaa cgccagatgt
      181 cgactcgctt atctcatccg cccgacatgt tgtacatgca atatgtgcgg attattattc
      241 attttcacgg aggttgctat gtcaaccgga ttacgtttca cactggaagt ggacggcctg
      301 ccgccggatg cttttgcggt ggtttccttt catctgaacc agttactctc ttcgctcttt
      361 tccctcgatc tctccctggt cagccagcag tttctctccc ttgaatttgc gcaggtgctg
      421 gacaaaatgg cctacctgac gatatggcag ggcgatgaag tacagcgccg ggtgaaaggc
      481 gtggtgacct ggtttgaact cggggagaac gacaaaaacc agatgctgta cagcatgaag
      541 gtgtgcccgc cgctgtggcg cacagggctg cgccagaact tccgtatctt ccagaatgag
      601 gacatcgaaa gcatcctcgc tacgatcctg aaggaaaacg gtgtgaccga gtggagtccg
      661 ctgttcagcg agccgcatcc ttcccgtgag ttttgtgtcc agtacggcga aaccgattac
      721 gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa
      781 aaaagtaccg accagagcct ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt
      841 gagatcccct ggaatccgaa cacccgtacc gaggtgagca ccctctgcat cagccagttc
      901 cgctacagtg cacaaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
      961 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cggtcagcac
     1021 cacgcagggc gggctgacgc gcagtatgga gtacgatgct gccggacgtg tcatcagtct
     1081 gactaacgag aacggcagcc acagcgactt cagctacgat gcgctggacc ggttggtaca
     1141 gcagggcggc tttgacgggc ggacgcaacg ttatcattat gacctgaccg gaaaactcac
     1201 gcagagtgaa gatgaggggc ttgtcaccct ctggtactac gatgaatcgg accgtatcac
     1261 tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag tatgatgacc acggctggct
     1321 gacagacatc agccacctga gcgaaggcca ccgtgtcgct gtccactacg gctacgacga
     1381 taagggccgc ctgaccggcg aacggcagac ggtggagaac ccggagacgg gggaactgct
     1441 gtggcagcat gagacaacac acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc
     1501 ggacagcctg ccgccggtgg agtggctgac ctacggcagc ggttatctgg cgggcatgaa
     1561 gcttggcgac acgccgctgc tggagtacac gcgcgaccgc atgcaccggg agacggtgcg
     1621 cagctttggc agcatggcag gcagtaatgc cgcatataaa ctgaccagca catacacccc
     1681 cgcaggccag ttacagagcc agcacctgaa cagcctggtg tatgaccgtg actacgggtg
     1741 gaatgacaac ggcgacctgg tgcgcatcag cggcccgcga cagacgcggg aatacggcta
     1801 cagcgccacg ggcaggctgg agagcgtgcg taccctcgca ccggacctgg acatccgcat
     1861 cccgtatgcc accgacccgg cgggaaaccg cctgccggac ccggagctgc acccggacag
     1921 taccctcacg gcgtggccgg ataaccgcat cgcagaggat gcgcactatg tttaccacta
     1981 tgatgaatac ggcagactga cggagaagac ggaccttatc ccggcgggtg ttatccggac
     2041 ggacgacgag cggacccacc actaccacta cgacagccag caccgcctgg tgttccacac
     2101 gcggatacag catggcgagc cactggtcga gagccgctac ctctac
//
LOCUS       DAPHDX010000279         2131 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPHDX010000279 DAPHDX010000000
VERSION     DAPHDX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.283"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1393..2052)
                     /locus_tag="RH058_004524"
     CDS             complement(1393..2052)
                     /locus_tag="RH058_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139970.1"
                     /translation="MLFLLTQLDFSEGTSDLVEMLMLLALTAAIVTIGILPGAIHLNE
                     RRNGDKFTGFIVVLICIPVVITVFSMWYRPIPNMIINMVMSLSGISDWRTHQYYIENK
                     THSHSMFNGLIWNTRHYTEIPERFFITGVSTFTLGDIKLVCPTEITEARKESLKFTIN
                     DINEYEQKGKQLKAVAMKCIPFNKNDIHTWDSPLSEPIYYEKVKQTIDNSMLKILHTL
                     K"
BASE COUNT          595 a          463 c          522 g          551 t
ORIGIN      
        1 tgtaatggtt gcctccggcg cagtttgcgg tgatgccgtt cccgggaatg gattgccagt
       61 gcggggacag tggagcttaa ttccggtatt ggtggccttt ccgttgattt tgagccaaca
      121 gccctcctgt gactgacagg ctgcctcgtc attccatacg tatcttcagg tgaacagatg
      181 catgtctgag cgtactcacg cagccggccc ggcaaccgcg gtggtactac gctggctgcg
      241 agaaaataca gaaagcacca gaatacgctc tcctgttgct gatcaatcag gatctcatgc
      301 acctaccggg atctagggct agaactgatg aaaccgattc gcacctggct gagttaatgc
      361 tacaggccag ggcatcacta ccccctgacg agcactgtac tgtggtatgc agttacaggg
      421 ggcgtgtgca gccacacctg aaagcagtga gtcgttcacg gcattcaaaa acacgtgcct
      481 ttcgttcgcc gttcctgtga tggccggcat catttgctaa gccgttaccg acgttattcc
      541 ctccgggaca cgcctctgat tgaggctttg cccggagcaa tcgaatgaaa ataagataag
      601 tcaccgaaag ggaggatgta gtcaaccggg ggaacagtaa taagccgtcg tgcttttatc
      661 gtacaagtgt gttgcgtttt gcaccgaaaa tacgatagag gtcgtttatg gattccgcag
      721 ggagtgctga ttattattca ctggcagcag acagcgatgt gtgtgcaggc attcaggggg
      781 atgttgtcag cagaagggtg agtatagcag attgttgtta tacatggcgt atgaagtatt
      841 accagtcatg cggagtagtg tggtgagggg ttgtatgaga aatcgccacc ttcttgtcag
      901 agagggaacg ctaccatgaa agtcaaaagc gataacgcag accggtacga tcggtaggta
      961 aaactgtagc ggtgtgctgc cgacgaggtc agaataatct gcgcgttttt acgccagcgc
     1021 caccgacgaa cagcaggaga ttgaggggaa tttgacggcg ataaagacgt agaagagaaa
     1081 aacgggcaat ttgccgcatg cccacagaat cagtgagatc gttagcgaca gcaaaatgat
     1141 cggcagccag cggcggcctg caatatcggg atgaaggaga tcctacgata tcgatgctgc
     1201 acctgatatg tacgatcagt aaatagagta cgaaacagaa aacacgacga taataacgct
     1261 ggcagcggtg cctgtcaggc aaacgcccac acgcaataaa ataaatcaac catcgcaggg
     1321 ccgtgatcat ccgcatacac cgctgatgac gcgtctaaaa acgcgtcaat atgacttccc
     1381 cggaacatta cctcatttca gggtatggag aatttttaac atcgagttat ctatggtctg
     1441 tttgactttc tcgtaataga tcggttcaga taatggcgaa tcccaggtat gaatatcgtt
     1501 cttattaaaa ggaatgcatt tcatggctac tgcctttaac tgtttccctt tttgctcata
     1561 ctcattgata tcattaatgg taaatttcaa actttctttt cttgcttctg ttatttcagt
     1621 tgggcatacc agctttatat ctcctaaggt aaatgtactg accccggtga tgaaaaatct
     1681 ctcggggatt tctgtatagt gccgagtatt ccagattaag ccattaaaca tactatgaga
     1741 atgagtttta ttttcgatat aatactgatg cgttctccag tcactaatgc cagagagact
     1801 cattaccata ttaatgatca tatttgggat agggcgatac cacatactga atacagtaat
     1861 tacaactggt atgcatatta aaacaactat gaatccagtg aatttatcac catttctgcg
     1921 ctcattaaga tgtatggcac caggcagaat gccaatagta acaatagctg ctgttaaggc
     1981 aaggagcatt agcatttcaa ccaaatcact tgtcccctca ctaaaatcaa gttgggtaag
     2041 cagaaataac ataggtaata tttgaacgat cccgggagtc ataaattgca agggtaaaaa
     2101 acataacaat cttggttttc ttggtaattt c
//
LOCUS       DAPHDX010000280         2045 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPHDX010000280 DAPHDX010000000
VERSION     DAPHDX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.284"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..495
                     /locus_tag="RH058_004525"
                     /pseudo
     CDS             1..495
                     /locus_tag="RH058_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
     gene            511..1026
                     /locus_tag="RH058_004526"
                     /pseudo
     CDS             511..1026
                     /locus_tag="RH058_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
     gene            1126..1695
                     /locus_tag="RH058_004527"
     CDS             1126..1695
                     /locus_tag="RH058_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139971.1"
                     /translation="MGKNNYPENKYRKWFSTAITYLITGGLCWYMLWASAISHYTVLQ
                     NTVPEWTTYFCTGIITGSLSFIYAFYIRTFNRTLKSLLNAFTGGFCLGFILSLNCYDV
                     CVYLFPDKVISYESEYDVVFPGPSRGKYGHCEAGLWLKDQNTSRWIQLCTNKEFLRSH
                     HKQGMTGVWVTARVNKIGSYIVKYEFIYM"
BASE COUNT          601 a          406 c          455 g          583 t
ORIGIN      
        1 aaccgcccgc attatgggcg ttggcctcaa cacgattttc cgccatttaa aaaactcagg
       61 ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg
      121 gacgaacagt ggggatacgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat
      181 gacaggctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg
      241 gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg
      301 ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag
      361 cgaattgagc ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg
      421 ctgtcgttct caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata
      481 aaacactatc aataagttgg agtcattacc gaatatgagt ttcagatttc tgacgaattc
      541 cggggagcga gggaacagtt cagggaaatc gttactcagt cgagaaacac cgcttttcag
      601 gctaacttac tgaaaacctc agaagaattt gccatcatat tccgcaagga gttaacttca
      661 aatattgtca atcgcctcgt tgcagatatt tctgtagtaa cgaacctgaa tggttttcag
      721 gaactggggt tcgggaaact gaatgcgtta tccgaaggac tcaaaaaact tctctttaaa
      781 gtcatcatgc gaaaacgtaa tatcctcacc tatgagttca ggggaaataa aataatcagg
      841 gatttatatg acttttacaa tgaaggagag aattataaat ttctgcctcc tgaacttaaa
      901 ttcaccttac cccaaccaga tttctgtata tttgaaatca gcaaaaaacg agcagtggtt
      961 gactatattt caggtatgat ggatacattt gcagtcaagg aatgggaaac tcactgtctg
     1021 aagtaacact gccgattacc agaggcgcat attttcccgt tatggttatt gcgcctttat
     1081 tccgtttcgg tgacaggtta ttttaaatat tctggaaatg cgttgatggg caagaacaat
     1141 taccctgaaa ataaatacag gaaatggttt tccacagcaa taacatatct tattacaggt
     1201 ggtttgtgct ggtatatgtt gtgggcatcc gctatatctc attatactgt tttacaaaat
     1261 acggtacctg aatggacaac atatttttgc accggtataa tcaccggatc tctttctttt
     1321 atttatgcat tttatatccg cacctttaac agaacattaa agtctttatt aaatgcattc
     1381 acggggggat tttgtcttgg atttatactg agtctgaact gttacgatgt ctgtgtttat
     1441 ctttttccgg ataaggttat tagctatgaa tctgaatatg atgttgtttt tccgggacca
     1501 tcaagaggaa agtatggaca ttgtgaagcg ggactctggt tgaaagatca aaacacttcc
     1561 agatggatac aactttgtac aaataaagag ttcctgcgca gtcaccataa gcaggggatg
     1621 acaggtgtat gggtaacagc tcgtgttaat aaaattgggt cgtatatcgt gaaatatgaa
     1681 tttatctata tgtaggtaaa agctgacatg tttaccatgg atgagttgct atatttttgc
     1741 gcatacagga gtaatcgatt actggctata ctaaaaatac agagaaaagc tatcccggga
     1801 taaagattac caactcacag agacaagcct tgccttataa acagataaat atcatcaaca
     1861 cagattcaga agctgaagca gtaagccatg gttaactgcc tgctgccagt acagataaaa
     1921 caatcagtct cgctgactgg ctcttatcac tatcgttatt tcataactct acagtataac
     1981 tcatttatct ttattgccat atattagcat gaagtcaggc attttcattc cctgtcgata
     2041 atata
//
LOCUS       DAPHDX010000281         2016 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPHDX010000281 DAPHDX010000000
VERSION     DAPHDX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.285"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..751
                     /locus_tag="RH058_004528"
     CDS             11..751
                     /locus_tag="RH058_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3139972.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            786..>2016
                     /gene="htrE"
                     /locus_tag="RH058_004529"
     CDS             786..>2016
                     /gene="htrE"
                     /locus_tag="RH058_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDV3139973.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAG"
BASE COUNT          571 a          432 c          446 g          567 t
ORIGIN      
        1 gggatggttt atgtttttta acaccaaaca tacaacagct ttatgcttcg taacctgtat
       61 ggcttttagt tcatcgtcta ttgcggacat tgtcatttcg ggtactcgcg taatatataa
      121 aagcgatcaa aaaagtgtca acgtacgtct ggaaaataaa gggaataacc cgttgcttgt
      181 ccagagttgg ttagatactg gcgatgacaa cgctgagcct ggcagtatta cagtcccttt
      241 tactgccacg ccgccagtat cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat
      301 gtacacagcc agcacctcac tgcctaaaga cagagagagc gtgttctggt ttaacgtact
      361 ggaagttcca ccaaaaccag atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc
      421 atttcgcaca cgtataaaac ttttctatcg cccggatgga ttgaagggaa atccctctga
      481 agccccgtta gccctgaagt ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt
      541 gaccaatcca accccttact acgtctcttt tagcagtggt gatttagaag ctagcggtaa
      601 acgctatccg attgatgtga aaatgattgc accatttagt gatgaggtca tgaaagtcac
      661 tggccttaat ggcaaagcaa gttctgcaaa agtacatttt tacgccatta atgattttgg
      721 tggcgccatt gaaggtaatg ccagtctgta atcaggcaag gatataattc cgcaggaagc
      781 ataacgtgac tataaaacca actaaaaatt atcataatca tctgacccgt atcgctacgt
      841 tttgcgcgct gctgtattgc aatagtgctt tctgtgctga acttgttgaa tatgaccata
      901 ccttcctgat ggggcagaat gcctcaaata ttgatctcag ccggtatagt gaaggtaacc
      961 ctgctatacc gggtatgtac gacgtcagtg tttatgtcaa cgatcaacct attattaacc
     1021 aaagcattac atttattgaa attgaaggaa aaaaaaacgc acaagcctgt atcacactta
     1081 agaatttatt gcagtttcat ataaattctc ccgatataaa taacgaaaaa gccgttctgc
     1141 ttgccaggga tgaaacactc ggcaattgcc ttaatttgac ggaaattatc cctcaggctt
     1201 ctgttcgtta tgacgttaac gagcaacgtc tggatataga cgttcctcag gcatgggtaa
     1261 tgaaaaatta ccaaaactat gttgatccat cgttatggga aaatggcatt aatgcggcca
     1321 tgttgtcata caacctcaac ggatatcata gtgaaactcc tggtcgaaga aatgacagca
     1381 tttatgctgc ttttaacggt gggatgaatt taggagcatg gcgactgcgt gcctcaggca
     1441 actacaactg gatgaccgat tctggcagta attatgattt taagaatcgg tatatacagc
     1501 gtgatatcgc ctcgctgcgt tctcaactca ttcttggtga gtcttataca acgggcgaaa
     1561 cctttgattc tgtcagtatc cgcggcattc gtttatacag cgacagccgc atgttgcctc
     1621 cgacattagc gagttttgcg cctatcattc atggcgttgc taataccaac gccaaagtca
     1681 ctattacgca aggtggctat aagatttatg aaacgactgt gccgccaggc gctttcgtca
     1741 ttgatgatct gagtccgtca ggttacggca gcgatcttat tgttactgtc gaagaatccg
     1801 atggctcaaa gcggacattc tcgcaacctt tttcatcggt cgttcaaatg ttacgccctg
     1861 gcgttggacg ttgggatatt agcggcggcc aggtcttaaa agatgatatt caggatgagc
     1921 ctaatttatt tcaggccagc tactactacg gtctgaataa ctatctaacg ggttatactg
     1981 gtattcagat taccgataat aactataccg ctggtt
//
LOCUS       DAPHDX010000282         1923 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPHDX010000282 DAPHDX010000000
VERSION     DAPHDX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.286"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..926
                     /locus_tag="RH058_004530"
                     /pseudo
     CDS             1..926
                     /locus_tag="RH058_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045641.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1270..1425
                     /locus_tag="RH058_004531"
                     /pseudo
     CDS             1270..1425
                     /locus_tag="RH058_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1462..>1923)
                     /locus_tag="RH058_004532"
     CDS             complement(1462..>1923)
                     /locus_tag="RH058_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3139974.1"
                     /translation="LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSR
                     HSELAKAFAYALNQWSALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGE
                     REALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          466 a          450 c          536 g          471 t
ORIGIN      
        1 ccggtacgtc acctgaccaa atcggctgaa gccgtgctgg gtatggcaac cgcgccagtt
       61 tatattttta acagtgaact gcagaatcta cgtttccgtc atggtgatgt catagagaac
      121 acccgtttac cgggtggagt ctggggacgt tatatggggt cagataaacg catcagtggt
      181 gcagttggtg ctggctatac gttgacacag aacggaatgg aaacaggcgg agataaggtt
      241 tttgagtttg gcgacagcca actggcagtg ggtgcctttg tttcctacac tgataacagt
      301 gtcagccata atcacggagg aaacagcaag gttggcagca cggggggggg ctgtatgcaa
      361 catggtttga taatgacggt tattacgtgg atggggttat caaattcaac cgtttcagca
      421 ataagcttcg cacctggatg agcgacggtg tggcagtgaa aggtgattac acccagaatg
      481 gttttggcgg tagtctggag gcaggtaaaa ctttcactct gaatgaaaat gcctgggtac
      541 agtcttatgt acggacaacg gcattcaggg caaatgcaaa aaatatccgt cttgataatg
      601 gtatggcagc caggatagaa gcagccaggt cgcttcaggg agaggccgga gtgaatctgg
      661 gcatgaatct tgatgtaaat ggcgtgactg tgaagcctta tctgacagcg gcagtgagtc
      721 atgagttttc ggacaagaat aaagtcagga taaacaacag ttatgacttt atcaacgata
      781 tatcaggtac gacggggaaa tatggggccg gggtgaatgc tcagttgacg ccaaacgccg
      841 gtatatgggc agaggctggc tatcagaaag ggaataagat ggagtcacca gtgacaggaa
      901 ctgtcggttt ccgcataaac ttctgactgt agccagaaat accagaccgg gggagcgttc
      961 cgcccccgga ttttagcctg tacagagatt tgtgtaattg cctgattttg agtgagcgta
     1021 aaccgactgc cgtatgtaac cattaagcca gattactgat gcagtgaaga ccttcccgca
     1081 tcctgactca cacaacaatc gactctttgt gtcctgtctg gacctgtcgg ttgccggaag
     1141 cgccttcatg cgaggcgtct cctcaccgat gcgcgtgact caagaagggc ctgacggctt
     1201 gtctcgttac tgtcctgtcc gggttatctg tctggagatt caactctgtt tcctcacagg
     1261 agctctgtca tggctgataa atataccgaa gccgctgttg tgggtggcgt ggatacacat
     1321 aaagatctgc acgttgctgc tgtcgtagat gagaacaata aagtccttgg gacccggtat
     1381 ttctccacaa cacgacaagg ttaccggcag atgctggcct ggatgtaagc gtacagcgag
     1441 agccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagtagttc
     1501 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     1561 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cttcccgctc
     1621 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     1681 ggcattttca gcgatgttgt tgtcgatttc cacccagcca tcgttcgcat agtacgtcag
     1741 tgccgaccac tggttaagtg cgtacgcgaa cgccttcgcc aactctgagt gtcgcgacag
     1801 ggtcttcatc ttttcacgca accagctttc cagggatttc aacagcggtt tcgtttttcg
     1861 ctgacgttca gcaagccgct gctctgccgg cattcccctt atatccgcct ctatggcgta
     1921 caa
//
LOCUS       DAPHDX010000283         1867 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPHDX010000283 DAPHDX010000000
VERSION     DAPHDX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.287"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            29..165
                     /locus_tag="RH058_004533"
     ncRNA           29..165
                     /ncRNA_class="other"
                     /locus_tag="RH058_004533"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(358..1344)
                     /locus_tag="RH058_004534"
     CDS             complement(358..1344)
                     /locus_tag="RH058_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139975.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1341..>1867)
                     /locus_tag="RH058_004535"
     CDS             complement(1341..>1867)
                     /locus_tag="RH058_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV3139976.1"
                     /translation="VKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQASLNEFA
                     TLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVA
                     PSIIKENLSLHLTHTVKWTESLDIFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRV
                     MHCRKAFSDAKVFK"
BASE COUNT          585 a          455 c          383 g          444 t
ORIGIN      
        1 cgtacgaaag tacgggcttt tttttcgtat attgcacaca ccggggggat gagaagcccc
       61 gaccggggtt cgacaactgg cgacagccag ttggacagac cgtgaacgca gtgagcgggc
      121 tgcccgcagg gcgagcgaag cgagtcaatc cccccctcac cgccatattt aaagaagagc
      181 ccgtacgaaa gtacgggctt ttttttcgta tattgcacac accgggtgcc cccgcccacg
      241 tctgttaggg cgaggggaaa ttgtgcacat caactgtctg gttactcagt caacaatcaa
      301 aatgcgcaag atagtagaga acatatatgc gttatctggt cacttcctgt aacttcctca
      361 cttattaaaa tgcccccaac ggaaatacat gtcacatgcc atcataaaat cgttatgcca
      421 gaggcatcga caatggaaaa aatagtaaat taagcacggt acatagcaga gtaaaaaata
      481 ggcttcaaac caaatccgcc attctacaag cgttgccaat accgacacca gcgtaatcca
      541 catcatttca ataaccagca atatgaataa ccgaagccaa agcggcttgg caaaaaaggt
      601 cgacaacgca ttttcactgg agaaaaacag atcgatttcc tgcaaaaaaa gcctgcccag
      661 aaaagcgtac cgcttggaga agcgcagaaa aaacagatta atcgccaaca ctacgccaca
      721 aagaatgagc aatgcaatta tcgatttaaa cgttaaccac tgcccctctg tcacaaaaga
      781 atcactgtta cgtaacgggc tgatagtaag caggttaaca accgttgaga tgcacaccga
      841 aagcagcaca tagcgagtca gacttttata ttttactttt ttcgctactg aaatagcgac
      901 ctgcttgaca taactggtgc ggatcatccg aaacagcaga aagatgacca acggcccaag
      961 taacgaaatc aggaacagcg catataacga gggtagaagc tggtatagcc cccaactcat
     1021 tgcactcaac atgattaacg acagaaatcc tgccaccaga ggttgtgtaa gaaataattc
     1081 tattttgtgg ttctgctttt tcttaaacca cgaaaaatcg atattcttcg tgacgcattt
     1141 ataagcccag tacgccttga gatcaaaaat aaaattatac agcaccaggg ccagaatccc
     1201 actaacgaca ccaatatcct ctgatggaaa agtaaatcct gcatttttcc aataaaagcc
     1261 tatcagaata agtccaatag cataaatgta tatcagattt cgtttatcct gaaagcgaat
     1321 aaaagttaaa taatcaggaa tcatttgaat acctttgcat cactaaatgc tttacgacaa
     1381 tgcataactc gttgctgatc cacgccagca aaatcattta acatattccc caaatagggc
     1441 ttattactta tttcaaggaa agctaccggg gttggcatat tcagaaatat atcgagagat
     1501 tcagtccatt tcaccgtatg ggttaagtgt aatgagagat tttctttgat aattgacgga
     1561 gcgacctcgc tttttgctgt tacgttcatc agaacctgat gctctggcga agcgatatcc
     1621 agtccggcaa gataatcgcg cattgcctga acgccgtctt ccatcaaacg cgtgtgccag
     1681 gcaccgctca caccgagttt aaccggttcg tatccagcgg ccatcagcag cgtggcaaat
     1741 tcattcaacg aggcctgcgt tcccccaata acctgctggc gcggcgtgtt atcacagcta
     1801 atatccagcg caatgcctga ttccgtaatc atcgtctgca gttgttcgcg gttgatgcct
     1861 ttaaccg
//
LOCUS       DAPHDX010000284         1845 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPHDX010000284 DAPHDX010000000
VERSION     DAPHDX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.288"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1287
                     /locus_tag="RH058_004536"
     CDS             <1..1287
                     /locus_tag="RH058_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3139977.1"
                     /translation="VNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYD
                     RRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCIL
                     IFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELTN
                     ENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMS
                     RYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFNEL
                     TVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARV
                     GGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENAL
                     NPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            complement(1328..>1845)
                     /locus_tag="RH058_004537"
     CDS             complement(1328..>1845)
                     /locus_tag="RH058_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDV3139978.1"
                     /translation="LLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIG
                     WSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMS
                     AKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGL
                     SPEQFENKNLA"
BASE COUNT          499 a          379 c          412 g          555 t
ORIGIN      
        1 gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg ttccacgatc aacctatctg
       61 atggtaatga ttgctttatt ttttatcgat actgttgcat ttatttttat gcagttgtat
      121 ttcatttatg accgtaggca attttcaaac tgtatactta gtttggcttt tctgagttgt
      181 ttgatttact ttgttaaaac cgtcattatc attcagcaaa ttattgaggg gcgtttgaca
      241 agcagtgttg tccaaaatga cattgcaatc tattatttgt ttcgtcagat gagtttgtgc
      301 atattaatat ttctggcatt ggtgaataaa gttagtgaaa acacaaaaca gcgcaattta
      361 ttttccaaaa aaatgacttt gtgcataagt ttgttttttg tttttggggg gccgatagtt
      421 gctcacatac tttctagtca ttatgagagc tacaatttac acatagccga gttaaccaat
      481 gaaaatggtc aggtggtttg gaaagcctca tatgttacca taatgatttt catgtggtta
      541 accttgcttt cggttaatct atactttaat ggtttacgct atgatatctg gaatggagtg
      601 acggtgattg ctttttgtgc cgtactgtac aatatctctc tgttatttat gagtaggtat
      661 agcgtgtcca cttggtatat tagtcgcact attgaagtag tcagtaagtt aaccgttatg
      721 gtaatattta tgtgccatat attcagtgcg ctacgagtaa caaagaacat tgcacatcgc
      781 gatcccttaa ccaatatatt taacagaaat tattttttta atgaactgac agttcaatca
      841 gcatcagcca aaaaaacgcc ttattgcgtc atgattatgg atatcgacca cttcaaaaaa
      901 gtcaacgaca cctgggggca tccggttggc gatcaggtga taaaaacagt ggtgaatatc
      961 attgggaaaa gcatacgacc agatgacctt ttagcgcgcg tcggcggcga agagtttggc
     1021 gtcttgctga cggacatcga tactgaacgt gcgaaagctt tagcggaaag gattcgggaa
     1081 aatgttgagc gtttaactgg cgataatcct gaatacgcta taccacaaaa agtgacgatt
     1141 agtattggcg ctgttgtgac tcaggaaaat gcgttaaacc caaatgagat ctatcgactg
     1201 gctgataacg cactttacga ggcgaaagag accgggcgta ataaggtggt tgtgagggat
     1261 gtggtgaatt tttgtgagtc accataaagc ggcattttga tcctacccac gtaatatgga
     1321 cacaggccta agcgaggttc ttgttttcaa attgttccgg actgaggccg ccacaccaac
     1381 tgtgccgccg ccaccgattg taatcacatt cgatataatt aaacaccgtt gcccgcatta
     1441 tttcccggct gataaagtgt tctccatgga tacattccac tttcagcgaa tgaaagaagc
     1501 tttccacgca ggcattatcg tagcagcaac cttttgcgct catacttcca cgcagattat
     1561 gccgcttcag ttgcgcctga taatctgctg aacagtactg gcctccacgg tccgtgtgaa
     1621 cgataacgtt ccggggcctc ttacgccgcc acagcgccat ctgcagggca tcgcaggcca
     1681 gttgcgccgt catgcgtggc gacattgacc agccaataac ggcacgtgac cacaggtcaa
     1741 tgaccactgc cagatacagc cagccttcat ctgtacgtaa gtacgtgatg tctcctgccc
     1801 acttctggtt cgggccactg gcgtaaaaat cctgctccaa cagat
//
LOCUS       DAPHDX010000285         1815 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPHDX010000285 DAPHDX010000000
VERSION     DAPHDX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.289"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(49..372)
                     /locus_tag="RH058_004538"
     CDS             complement(49..372)
                     /locus_tag="RH058_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV3139979.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            636..722
                     /gene="azuC"
                     /locus_tag="RH058_004539"
     CDS             636..722
                     /gene="azuC"
                     /locus_tag="RH058_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV3139980.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            837..1088
                     /locus_tag="RH058_004540"
     CDS             837..1088
                     /locus_tag="RH058_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV3139981.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1167..1670)
                     /locus_tag="RH058_004541"
     CDS             complement(1167..1670)
                     /locus_tag="RH058_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV3139982.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT          509 a          404 c          430 g          472 t
ORIGIN      
        1 taccgctatt tctttaatca gcgaagatcc cctcgctgat taaaatattt agagaaagac
       61 agggaaggcg gaatgatggc actgatatga caaagtgaat tccgtattca aacataagga
      121 acccgcaaaa aggctacagt tgcctactgg ctgaccaaag gggaccgcgt gggtatagcc
      181 ttcctgctgg catgcgcgat cggcgatccc gcgactgaca taacgatcgg tatgggcatg
      241 ctgcaaaaaa gcctggtcat aaactaaccg tacaatacct gttgccgcgt caacttcgct
      301 cacatgggcc tggcgagtaa ccgtacaccc cgccagcgtt attaatgaaa gagtcagaat
      361 cagaagtctc atggtccagc cagaagagta agagaaagca ctattttacg cgttacagtg
      421 ctgcgacaat ggccggaaaa gacgcctggc aggcgctatt tcttgccatc tgtaggtgtc
      481 agaaataggg ggggctgttt tttgattatc atttttataa cattaaaggg gtgtttatgt
      541 gatttgtatc acataaagat tgatttttgt gatggttgtc atattataag cgcaaagaca
      601 ataacacctg tataacaaat ggtcggagtg ccgcgatgaa actgcgcaaa atcctgaaaa
      661 gtatgttcaa taactattgc aagacgttca aagacgtacc gccaggcaat atgttccgat
      721 aacaaaaaac ctgctccggc aggttttttt gtgtcctgat gacggtggat gtgactatcg
      781 ctgaagatgt gaagccgcta ctataacggc tttcaaaatg ctatcaggag tttacgatgt
      841 cccagccgct gaatgccgat caggaactgg tttctgatgt ggttgcttgc cagttggtta
      901 tcaaacaaat tctggatgtt ctcgatgtaa tcgcgcccgt tgaagttcgt gaaaaaatgt
      961 ccagtcagct gaagaatatc gactttacta accatcccgc cgccgcagac ccggtcacca
     1021 tgcgggcaat ccaaaaagcc atcgcgctta ttgaactcaa gtttacccca cagggtgaat
     1081 cccattaaaa aaaacgctgc ttcaaacaga tgaggcagcg gtttctcaaa accttcatcg
     1141 gcgcaatgca ttagcgccga tgatgatcaa tgcagctggt gtgacacgac attcagaaca
     1201 tgttggtcgg tctgcacagg gcacataccc gcaagtttcg cactgcgcac ttcatcaaga
     1261 atggtttgca gcaacagacc atcatgctgc tgttcttttt ccagatcgcg caggaaattg
     1321 acggttgaat catcattcag ttctttcgct tcatcggcta actgtgccaa cgtactagaa
     1381 cgttgctcgt attcttccat cgttttttgg aacagttctt ccagagagtt cagtttttca
     1441 ccgggaacat caatggcttt aacgatgggg gtagcgccga cactcttcat aaagttaaac
     1501 atgcgcatca tttgggtcac attactctgt gcctgggcgc gaaggaaagt ggcggtgccg
     1561 ttcagactct gttcagaaca ccagttactc aggtgaaggt agagattgga tgcgtaaaac
     1621 tcgcggttca tttgagagtt gagtttgaga agcattccag cggttgccat acctaatatc
     1681 cttatatcca gaagtggaga ggtgcaagat aaatcaaaac accataatta ctgtttgcag
     1741 aataattcct ttctgccggt ccgaaatgtg atcgtggagt caattctgac gatgatctga
     1801 aataagaata acgtc
//
LOCUS       DAPHDX010000286         1808 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPHDX010000286 DAPHDX010000000
VERSION     DAPHDX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.290"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1808
                     /locus_tag="RH058_004542"
                     /pseudo
     CDS             1..1808
                     /locus_tag="RH058_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          631 a          356 c          337 g          484 t
ORIGIN      
        1 tcaacttttc acgcagaaaa agttattaat gatctgttaa aaaacgatta taaaatttcc
       61 ctttctccag atacaactta tcgtgagttg aataaagcag cacagcgtag cattacagcg
      121 ccagacagga taggagaagg aaaatcatgg gtttatcaaa gagatacaat gattgaaaga
      181 ggtgataaca gcggtgttca tcagtatggt cctgctgaac atttcaccca cattatatct
      241 gacaaaccgt ccccaaaaga tgagtatatt gcatatgcta ttaacattcc tgactatgag
      301 ctggcagccg atgtatataa tattaacgtg acgtcacctt ccgaacagca agaaacattt
      361 aaaatactaa tcaatccaga acatctacgg caaacacttg agcgtaaatc tcttactgct
      421 gttcagaaat cacaatgtga aatcataacc cccaaaaaac ctggcgaagc gattcttcat
      481 gcttttaatg ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat
      541 tatgggtata tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc
      601 gaagaagaaa agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat
      661 aatccaatgg gagatttgca ggattttatc gcattggtta aagatgctaa aaaatttggt
      721 atcgatatca ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat
      781 gacctggatt atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt
      841 atccctggct attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa
      901 gaacgtaaac aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt
      961 aaagataaca tatctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta
     1021 aatggaatgc ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac
     1081 gttcgtgaaa tgcgaaaata tggcgtacga ggtttacgtt acgatgcagc aaaacactca
     1141 aaacatgaac aaatagaaag atcaataact ccaccgctta aaaattataa tgagcggtta
     1201 cacaatacta atttatttaa cccaaaatat caaaaaaaag ccgttatgaa ttacatggaa
     1261 tacctggtaa cttgtcagtt ggatgaacaa caaatgtcat cgctgcttta tgaaagagat
     1321 gatttaagcg ccattgattt ttcattgctc atgaaaacga taaaagcctt ttcatttgat
     1381 ggcgatctcc aaacccttgc atcaaaaccg ggttccacaa tctcaagtat cccatcagaa
     1441 agacggatat tgattaatca ttaaccacga ttttccaaac aatggcaatc ttttcaatga
     1501 ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatag ccgctctgcc
     1561 gttcagcagg cctttagttt actgggatgg ccaagtatta aaatcaacga ctgaaattaa
     1621 aaattatgat gggtccacgc gtgtcggcgg tgaggcgtag cttaataaag gttgctctac
     1681 ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag gaatatggag
     1741 cgcatttgag ggtgtatctg caactaaaaa cgttctggcc tttagtcgtg gggattctgt
     1801 gaacatta
//
LOCUS       DAPHDX010000287         1799 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPHDX010000287 DAPHDX010000000
VERSION     DAPHDX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.291"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(686..925)
                     /locus_tag="RH058_004543"
     CDS             complement(686..925)
                     /locus_tag="RH058_004543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139983.1"
                     /translation="MEREFPEALERMKERLPLYLIMVLENQYNRLNELDSLIEDIEKQ
                     LTSVAKQNETCKRLLDIPGVGPLIATAAVAIIGEA"
BASE COUNT          494 a          423 c          378 g          504 t
ORIGIN      
        1 gtttgtttca tattataatt tttcataaaa atacggaaaa ccggattgaa gacagatatg
       61 cctgcagacc tggagggccg tggcgggata tggggcttta tatgaatgca tcccgtttac
      121 acagcttcca ttgttctggc attgacggta ggttgcagcc tttatatccg gcattattgc
      181 agccataaag ctgcgagccc ctatgtaatt cgctgactca cacttcattt actgcgcggg
      241 attcaagcca aaaaaacatt caggtttagt gagtcccggt cttacctgtc tttgtcatca
      301 ctacggctgc cctcgcaacc tttcagcatc cccaattttg ttaagagttc attgttgaac
      361 ggtatcagcg attaatatgg tctgatactg acatggttcc cgtcatactt acgaccatgt
      421 gcggctattg cccacactgt tcgtgccagc ttgtttgcca tggcgacgtt gccacgccga
      481 ccggacgacg ttttttcagt tcggttatcc acggcccagg ttctctggtc agtaatgctg
      541 ccgcccttgc accatgaata aacagggttc gcagatattt atcaccacgt ttgcttatcc
      601 ccggcaggcg gattttccct catgagccag tctgtcttgg ctccagacca gcataagccg
      661 cgaactctcg ccctgattta aacgcttatg cttcccctat gatagccacc gctgccgtcg
      721 caataagtgg cccaacgccg ggaatatcca gcaaccgctt acaggtctca ttctgtttcg
      781 ccacgctggt aagctgcttt tcaatatcct ctatcagtga gtccagctca ttcagtcggt
      841 tgtattggtt ttccagaacc ataatgagat acagtggcag tctctctttc atccgctcca
      901 gcgcttcagg gaactcccgt tccatcgctg cccggccttt gtggatggtt ttaccaaact
      961 ccagtagcgt cccgtgcagg gcattaacgg tctaacatcg tggcgttgac accaataaaa
     1021 ggagaaataa aaatacctac cataataaat ggcagataaa actgtgataa gggaggaaaa
     1081 caggtaggtg tatgctctcc tgtcggtcat caggtaatct gatacaagaa gatactgttc
     1141 agttaaaccg cgggagataa tctgttatga aatatgtgcg tcacttctca gaaatcgtgt
     1201 aataacaaac aaaattaatc aaaagaaatt catcgcaatg atatcccaat atgcctgtga
     1261 gcatataaaa aaagcccgag gacacttacg ggctgattga gcgacattga tattaagtaa
     1321 tgattttttc tttaaattct acgataaagg acgtggcttt aactttgtcg gaaatcacaa
     1381 caacacaacg aataaaatat agcgaggggg agacgtgttc atgggcattc gccagggatg
     1441 aataaaacag caacaaaaaa atgcccgtac ctctcgtacg ggccagcgtc acacattatc
     1501 atggagttgt cagcgggatt tattattagc tttgatggaa tatataatca gatactggcg
     1561 ttgttctgtg atttttatca cacctttaaa tgtattaaat cagccacaca tctttatgtg
     1621 ttcaccagtc caggtaatac cggatgcccc tgcatccaca tccgtaacag caaacctgaa
     1681 tcattgtagt ggaataacct ctcagaccag tgatacggat gggtaacagg atatatactc
     1741 tttaattgta tcagttcaaa aaaagctatt atttcacgca tttcttaaac atcagttaa
//
LOCUS       DAPHDX010000288         1797 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPHDX010000288 DAPHDX010000000
VERSION     DAPHDX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.292"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1329
                     /locus_tag="RH058_004544"
     CDS             <1..1329
                     /locus_tag="RH058_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV3139984.1"
                     /translation="TILSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTS
                     YQVAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNS
                     LSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDE
                     NNKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQ
                     LNYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGG
                     INITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANS
                     ESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENN
                     IGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1342..1797
                     /locus_tag="RH058_004545"
                     /pseudo
     CDS             1342..1797
                     /locus_tag="RH058_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          570 a          351 c          347 g          529 t
ORIGIN      
        1 acgattttat ccgataacta taatgctata actctgggaa atggttggaa tacaccgctg
       61 ggtgccatct ctttttacgc tgcccgatcg agtagtaagt taaataatga taccagacac
      121 gaaggtacga gctatcaggt cgcctataat aaatatctag tacaaacagc cactcgattt
      181 agcgtggccg cctggcgtta tgcttcgcag gattacagga cattcagcga ccatctttac
      241 gaaaatgata aacactatca tcagagtgat tatgatgatt tttatgatat tggcagaaaa
      301 aatagccttt ctgctaatat tatgcaacct ttatccaata atctgggaaa tgtatcatta
      361 agtgcgcttt ggcggaatta ctgggggcga agtggaaatg ctaaagatta ccaattcagt
      421 tactccaata actggcaaca cattagttat actttctctg ccagccaatc ttatgatgaa
      481 aataataaag aagaggagcg ttttaatctg tttatctcca ttcctttcta ctggggggat
      541 gatattgcca aaacacgtca ccaaattaac ttatcgaatt cgacctcatt ttccaaagat
      601 ggctattcct ccaacaatac tggaattact ggcatagccg gtgaacatga tcagttaaat
      661 tatggtatat atgttaatca gcaacaacaa aataatgata cctcgcttgg tacgaattta
      721 agctggagaa ctcccatcgc cataatagat ggcagctata gtcattctaa aaacgcctgg
      781 caaagtggtg gaagtattag tagtggatta gttgtctggc ccggtggtat taatatcact
      841 aaccagttat ccgatacatt tgcaattctg gatgcgcctg ggttagaagg tgcacatatt
      901 aatggacaaa aatacaatcg aacaaacagc aaaggccagg ttgtttacga cctgataata
      961 cctcatcgtg aaaaccatct ggtacttgat atagcaaaca gtgaaagtga aacagaattg
     1021 cagggcaatc gtcaaattat tgcaccttac cgtggagcag tttcttatgt gcagtttaca
     1081 actgaccaac gtaagccctg gtatatacag gcactgcgtc ccgatggttc gccattaacc
     1141 tttggctatg acgtactgga tctccaggaa aataatattg gagtcgttgg ccagggtagt
     1201 cgccttttta ttcgcgtaga tgaaattccc actggaataa aagttgctct caatgatgaa
     1261 cagaacttat tctgtactat tacttttcaa cacgttatcg atgaaaacaa aacatatata
     1321 tgccagtaaa aggagtttac tatgaagtgg cttttattaa tcaccttgag tctatattct
     1381 tttattgtgc aaagtgctcc ctgtgcattg accaatgtcg gtgaacaacg tggaacatat
     1441 atactcaagc cactgagtat gaaaggtaat ctaaccgccg aaaaattgaa aacaccggaa
     1501 atatcattga gtgtacagac ttgcaagaga caaccaacgc cattaaagtt cattttttaa
     1561 cacagaacgc tttaaggttt aaagtcggaa gtcgtcatta tattattaat ttcccctttg
     1621 aatctgacgg taaaaaaata gagcttccag ggaagtcatc ttataattta gaagaaatgc
     1681 tatctgcgat aaatttcacc attccatatt ctatcgcatc tgtatcagat agttctaaca
     1741 ccgcagacat ttttcctgga actccttttc ctttgttgtt cgaaattgaa gtgagtt
//
LOCUS       DAPHDX010000289         1775 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPHDX010000289 DAPHDX010000000
VERSION     DAPHDX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.293"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            34..1440
                     /gene="gndA"
                     /locus_tag="RH058_004546"
     CDS             34..1440
                     /gene="gndA"
                     /locus_tag="RH058_004546"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV3139985.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSRGKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCITYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNKELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKGEFSEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGKIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            1640..>1775
                     /locus_tag="RH058_004547"
     CDS             1640..>1775
                     /locus_tag="RH058_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3139986.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     C"
BASE COUNT          464 a          408 c          457 g          446 t
ORIGIN      
        1 tatcgcgcgg taaccccctg ataggagtaa acaatgtcaa agcaacagat cggcgtcgtc
       61 ggtatggcag tgatggggcg caaccttgcg ctcaatatcg aaagccgtgg ttataccgtc
      121 tctattttca accgttcccg tgggaagacg gaagaagtga ttgccgaaaa tccaggcaag
      181 aaactggttc cttattatac ggtgaaagag tttgttgaat ctctggaaac gcctcgtcgc
      241 atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc cctcaagcca
      301 tacctcgata aaggtgacat catcattgat ggtggtaata ccttcttcca ggacaccatt
      361 cgccgtaatc gtgagctttc tgcagaaggc tttaacttca ttggtaccgg tgtttccgga
      421 ggcgaagaag gtgcgttgaa aggtccttcc attatgcctg gtgggcagaa agaagcctat
      481 gagcttgttg cgccgatcct gaccaaaatc gccgcagtgg ctgaagacgg tgagccatgc
      541 attacctata tcggtgccga tggtgcaggt cactatgtga agatggttca caacggtatt
      601 gaatatggtg atatgcaact gattgctgaa gcctattctc tgcttaaagg tggcctgaac
      661 ctaaccaaca aagaactagc tcagaccttt actgagtgga ataacggtga actgagcagc
      721 tacctgatcg acatcaccaa agatatcttc accaaaaaag atgaagacgg taactacctg
      781 gttgatgtga ttctggatga agcggctaac aaaggtaccg gtaaatggac tagccagagt
      841 gcgctggatc tcggcgaacc gctgtcgctg attaccgagt ctgtgtttgc acgttatatc
      901 tcttctctga aagatcagcg tgtagccgcg tctaaagttc tctctggccc gcaagcgcag
      961 ccagcaggcg acaaaggtga gttcagcgaa aaagttcgtc gtgcgctgta tctgggtaaa
     1021 atcgtttctt acgctcaggg cttctctcag ctgcgtgctg cgtctgaaga gtacaactgg
     1081 gatctgaact acggtaaaat cgcgaagatt ttccgtgctg gctgcatcat ccgtgcgcag
     1141 ttcctgcaga aaatcaccga tgcatatgcc gaaaatccgc agatcgctaa cctgctgttg
     1201 gctccgtact tcaagcaaat tgccgatgac taccagcagg cgctgcgtga tgtcgttgct
     1261 tatgcagtac agaacggtat cccggttcct accttcgccg ctgcggttgc ttattatgat
     1321 agctaccgtg ccgctgtttt gcctgcgaac ctgatccagg cacagcgtga ctatttcggt
     1381 gcgcatactt ataagcgcat tgataaagaa ggtgtgttcc ataccgaatg gctggattaa
     1441 tctgatttaa ccaacaataa aattgaggcc cggcttatat tgcgtcgggc ttttttgtgc
     1501 acggaaaacc cccagctagg ctgggggttc cggaaagctt tcagctttga gccagttatt
     1561 aaaacccctt ttgatttgtt aaaacacctt gcggtctggc aactgcaagt gtcaaacaag
     1621 aaatcaaaag ggggtcccaa tggggaacga aaagagctta gcgcacaccc gatggaactg
     1681 taaatatcac atagtttttg cgccaaaata ccgaagacag gtgttctaca gagagaagcg
     1741 tagagcaata ggcagtattt tgagaaagct gtgtg
//
LOCUS       DAPHDX010000290         1735 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPHDX010000290 DAPHDX010000000
VERSION     DAPHDX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.294"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(62..1233)
                     /locus_tag="RH058_004548"
                     /pseudo
     CDS             complement(62..1233)
                     /locus_tag="RH058_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1350..1735)
                     /locus_tag="RH058_004549"
                     /pseudo
     CDS             complement(1350..1735)
                     /locus_tag="RH058_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          479 a          397 c          263 g          596 t
ORIGIN      
        1 cttaagttga ttaagtattt ctatagttca aacttccaca accgcagggc aagagcatga
       61 attaaatttg tgttaaattt ttcattttgt cagaccgaaa tccaggcatt gcgcaggatg
      121 caattacttt ctctatgtaa cttgtattta acgcagcctt ctttcttttt ctctttaccc
      181 cgcccttaaa acctttgcaa tctctcaata aattcagggc cactttcttt attccagata
      241 ttcttttggc tgcgtttcct cttcttattc tgcttccatc ttcattcatt tttacatcga
      301 gcacccaatg atgattattc tcgattttcc agtgagctct gatagcatgt gcaaattctt
      361 tagcatccat atcctttgat gaaatgtaat atcgaatgat taaaccttcc gctgatttat
      421 cttctttctt ttacctgaat gacaatgcca cacaaagctt ttttaatccc ttccattcaa
      481 attcaaaatc acaaaattca ggcgttacgt tactgataat atgtaaacgt gtttcttttc
      541 ttccatggct tatctcctga gtatcaaatg aatcgccttt ataattagag aacattttta
      601 cagggaattt ttcttcgaat gcatgatgta atttcccctg attgcctttt actgccagaa
      661 gataatctgc ctttttatct ttaattttag aagcgatatc tttctgacag cccatagcat
      721 caatggttat caaacttttc tttaaatcca gtaggttaag caactctgga atggctgtaa
      781 cctcattact tttggcttcc gttttcacct gccccagtac aataccattt tcggtcgaga
      841 atgcactcac catatggatt gctccttttc tttttccctt atcaaaggag cctcttatgg
      901 tctttccatc tattgctata atttcgccat cagtgatttc atggcattcc tgcatccatt
      961 caataaacat cttttcaaag gccaaactgt caatgttact cacaacgcgt gcaatggtgt
     1021 catccaccgg aatgccatta gcaaaatcac catatttctt tagccattcc agtctttcat
     1081 gtccaaaatc ttcaatttcc tgccactcat cggcaccagc aattactgca cagacggtga
     1141 ggagcaaaat atcagataat ttatgtctaa ctttccttgt tgtcgtccat cagggatcac
     1201 agaaatataa tcaagcaaac tttgaatact catctgggtg tccttttatc tttttagtta
     1261 ttccaaagat taaattttaa tcacccgtga gagttaaaac caggataaaa agagtgactt
     1321 atatcacgct tttcacgatc ttgccctgct ttaatatcca atatgttaag aagttcaggg
     1381 atagttgtga tctcattgga tttctcatct gtcttgatct gtccgatgac cagactgtgc
     1441 attgttgaga acgcactaat gacatgaatc gctcccctgc agcgactctt gtcataagaa
     1501 tgccggagtg ttttccatca attgcgatga cgtctttatc atttgaagaa tggcagtcac
     1561 gcatccagtt aataaagcac tcgtgaaatt ttgcaggact gatacaggat acaaccctgg
     1621 caattgtatc gtgaacagga ataccatttt caaaatcacc atattgcttc aaaaaatcga
     1681 gatgagtttc cccaaaaccc tctatatcac cccagccttc tgcaccagaa ataac
//
LOCUS       DAPHDX010000291         1683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPHDX010000291 DAPHDX010000000
VERSION     DAPHDX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.295"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..731
                     /locus_tag="RH058_004550"
     CDS             123..731
                     /locus_tag="RH058_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV3139987.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            876..1551
                     /locus_tag="RH058_004551"
                     /pseudo
     CDS             876..1551
                     /locus_tag="RH058_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          442 a          422 c          391 g          428 t
ORIGIN      
        1 atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat ggacgtttaa
      121 ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc tttgcctggc
      181 agccgcaaac tggcgacatc atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc
      241 aactggcaac ccattccgat tacagccaca ccggtatgct ggtgatacgc aacaaaaagc
      301 cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggatcgccc
      361 atggtgaaaa gggcaaatac gtcgttcgcc gcgttgaagg cggactgagt gttgaacaac
      421 agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat ttcagcttct
      481 catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc
      541 tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc aacccgctgg
      601 tccaggcgaa gctcaaagaa cgctacggta aaaatattcc gcttgaggaa acggtagttt
      661 caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag tggccgctgt
      721 tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta
      781 gttgcacgac ctttccgcaa gctgtcgctc cggtaaacgg tgatgcctcc aattagttga
      841 attaaggata taatactcgc ttttaaggtt gtttcatggt cacagttaac cttcattgcc
      901 cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaaaga catgatcgat
      961 tccgcggccg tgactgtcac cgggtatttc agctcagtta ctgttacgag ccccgtaaac
     1021 cgggagttaa agaccaaatc gctgaaatga ctttcaacgg tgccggggtt cgcgatactg
     1081 cccgtacgct gaaaatcggc attaatacag tcatccgaac tttaaaaaac tctcgccacg
     1141 aagaataacg tcatccccgg tcgctcatgc tgatatggca ctcatctgca aacttagtca
     1201 gcaatggagc tttgtcggca gtaaagcgcg acagcactgg ctctggtacg tgtacaacac
     1261 gaaaactggt ggtgttctgg cctacacttt tggcccccgg actgacgaaa cctgtcgtga
     1321 attgcgggcg ctgcttaccc ttttaccatc ggcatgttga ccactgacga atggggtagt
     1381 tacgctagag agttaccgaa ggagaagcat ctgactggta agatttttac tcagcacatt
     1441 gagcgtaaca atctgaccct gagaacccga atcaaacgtc tggcccgcag aaccatctgt
     1501 ttctcgcgtt ctgtagaact tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt
     1561 tctactaatt ggagtcatca cccttctgat aagctttcct tggatgatta ccagactgaa
     1621 tacacagcca aagattttta cctcattctg ctcttttttc tcaaatgaca tatattatat
     1681 ttt
//
LOCUS       DAPHDX010000292         1639 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPHDX010000292 DAPHDX010000000
VERSION     DAPHDX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.296"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..135
                     /locus_tag="RH058_004552"
     CDS             28..135
                     /locus_tag="RH058_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3139988.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            341..1624
                     /gene="gltA"
                     /locus_tag="RH058_004553"
     CDS             341..1624
                     /gene="gltA"
                     /locus_tag="RH058_004553"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV3139989.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          424 a          400 c          390 g          425 t
ORIGIN      
        1 tacaaacatt accaggaaaa gcatataatg cgtaaaagtt atgaagtcgg tatttcacct
       61 aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac agttattagt
      121 ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta ttcgtcatca
      181 atggatcctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc tctttcacag
      241 agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc aaatttaagt
      301 tccggcagtc ttacgtaata aggcgctaag gagaccttaa atggctgata caaaagcaaa
      361 actcaccctc aacggggaca cagctgttga actggatgtg ctgaaaggca cgctgggtca
      421 agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg acccaggctt
      481 cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg aaggtatttt
      541 gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc tggaagtttg
      601 ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat ttaaaactac
      661 ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg ctttccgtcg
      721 cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg cgttctatca
      781 cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc gcctgctgtc
      841 gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc catttgttta
      901 tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct ccacgccgtg
      961 cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc tgatcctgca
     1021 cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct cttcgggtgc
     1081 gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg cgcacggtgg
     1141 tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac acattccgga
     1201 atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct tcggtcaccg
     1261 cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc atgaagttct
     1321 gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg aaaacatcgc
     1381 gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt tctactctgg
     1441 tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt tcgcaatggc
     1501 acgtaccgtt ggctggatcg cccactggag cgaaatgcac agtgacggta tgaagattgc
     1561 ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg atatcaagcg
     1621 ttaatggttg attgctaag
//
LOCUS       DAPHDX010000293         1637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPHDX010000293 DAPHDX010000000
VERSION     DAPHDX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.297"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..427)
                     /locus_tag="RH058_004554"
     CDS             complement(<1..427)
                     /locus_tag="RH058_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3139990.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIV"
     gene            complement(436..1203)
                     /gene="sctT"
                     /locus_tag="RH058_004555"
     CDS             complement(436..1203)
                     /gene="sctT"
                     /locus_tag="RH058_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV3139991.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTI
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1205..1464)
                     /locus_tag="RH058_004556"
                     /pseudo
     CDS             complement(1205..1464)
                     /locus_tag="RH058_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
     gene            complement(1474..>1637)
                     /locus_tag="RH058_004557"
     CDS             complement(1474..>1637)
                     /locus_tag="RH058_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV3139992.1"
                     /translation="LVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLIL
                     QYFDLSINP"
BASE COUNT          564 a          333 c          282 g          458 t
ORIGIN      
        1 aaacaatata aagaattgct ttaacaaatt cttttacggt ttttaagcca aatatacgct
       61 ttaagccatt tactggattc aatgaatcaa acttaaattt aacagcttca gttgctaatt
      121 gcattttact ttgaaaccag gagacaagta ttgttgtgaa catgcaaacc agcaaaaaag
      181 gaatgttcgt tttcaaccaa ccaaccccca tagctttaaa atagtcccaa atatctggtt
      241 tagcgttatg atcaaggatg tattcaagaa tcgacataat gtgatgcaca tcaaaaacat
      301 atccgagata taaagtaccc acaagcatta ttacagagat ggttaagtct ctacttttta
      361 gaatttgccc ttttttagaa gcatcctgga gtttcttttg tgttggcttt tcagttttat
      421 tggccatgaa acactctatt tctcaatgta tagttgcaat tgttctggga aaaaggataa
      481 cggcattact cgttcagcca gtattggggc aaaataaata attaatatta aaaatgccag
      541 cgcacttttg atagtcagtg aaatggcaaa agcatttagt tgtgaagcat aacgtgccag
      601 caatcccagg acagcctcac cgcccagcat aacggcaatc acggggcttg cgtaaacgat
      661 agtatgagtc attatgttat taatatatga gaataaaggc tctaatttag gaaaattaaa
      721 attgcctgaa ggccataaat tataactttg ccaaagagtt tccagaataa agttcattcc
      781 acctttagta agataaaccg cagcagagaa aaggttaaat aatcttgcta attccgaggt
      841 atcgacacct gtagccggat ccaaagtact acttaatgtt gcaccgcgtt ggttatcgat
      901 aaagctacct atagcaagga aaatccaaaa cggggatgct aataagcatg caataaacag
      961 ccctatgata atttctctgg cagcgaacaa agcaatatcg atttcattta agttcaataa
     1021 ttcatagtgg taatgctgaa ctaatgcaca tgataccact attattattg ggtgacgaac
     1081 tactgttggt atattcccac tggcgaaaaa tggtaacaaa tagaaagttg gtgccaatcg
     1141 gcaaaaaccg agcgctgtag cagccagcag actatgcaac tgatagagta tcgcctcccc
     1201 catgctatcc tctcgcgaat gctaaattca gcatttcgat accaaatgaa tataattttt
     1261 caccatacca cccgctcatt aaaaaaagca aatacttacg cacaataatt tcacgccaaa
     1321 tggcaacgtc tgttcttgca actgcgttac cgtttgaaaa agccctacca ataaaccaac
     1381 aaatgtcgca actgcaatag gtcctgcaga cataactaaa atcagataca gcgccctatt
     1441 ccccgcaaaa acaatatcgt ccatattttt tcctcaggga ttaatggata ggtcaaaata
     1501 ttgtaaaatg agcccttttg ataacattgt ccaaccgtcc atcgcgacaa acaaaattaa
     1561 ttttatcggt gtagatatgg ttaccggact catcatcatc atacctaacg ttaacagaac
     1621 gctggatata accagat
//
LOCUS       DAPHDX010000294         1614 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPHDX010000294 DAPHDX010000000
VERSION     DAPHDX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.298"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1088
                     /locus_tag="RH058_004558"
                     /pseudo
     CDS             1..1088
                     /locus_tag="RH058_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1185..>1614
                     /locus_tag="RH058_004559"
     CDS             1185..>1614
                     /locus_tag="RH058_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3139993.1"
                     /translation="MTHSTIKQLTIKAILEYIEDNIEVKSIDIESIVCFSGYSRRYLQ
                     SLFKSHMGVPIGRYIQLRRVSRAAVLLRLTSLSLASIAGKLCYDSQQTFTREFKKNSG
                     FTPLQYRNSNLWTFKNLTGPKEPSIVIPKPKLCYLDENKFK"
BASE COUNT          460 a          353 c          426 g          375 t
ORIGIN      
        1 ctctggcgta agcaatacca ggaaggaagt cttactgctg tggccgccgg agaacaggtt
       61 gttcctgcct ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc
      121 ggcaagaaaa cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa
      181 aagtggatag cgcacgcgcc cttattgccc ggggatgggg agtaagcttc gtcagccgtt
      241 gtctccgggt gtcgcgtgcg cagttgcacg tcattctcag acgaaccgat gactggatga
      301 atggccgccg cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta
      361 tcggagagct gcccacgtat ggttatcgtc aggtatgggc gctgcttcgc agacaggcag
      421 aacttgatgg tatgcctgcg atcaatgcca aacgtgttta ccggagcatg cgccagaatg
      481 cgctgttgct tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag
      541 tggctgtgaa agaaagtaat cagcgatggt gctctgacgg gttcgagttc cgctgtgata
      601 acggagaaaa actgcaagtc acgttcgcgc tggactgctg tgaccgtgag gcactgcact
      661 gggcggtcac tacgggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg
      721 tggaacgccg cttcggcaac gagcttccgg cgtctccagt agagtggctg acggataatg
      781 gttcatgcta ccgggctaat gaaacacggc agtttgcccg gatgttgggg cttgaaccga
      841 agagcacggc ggtgcggagt ccggagagta acggcatagc agagagcttc gtgaaaacga
      901 taaagcgtga ctacatcagt gtcatgccca aaccagacgg gttaacggca gcaaagaacc
      961 ttgcagaggc gttcgagcat tataacgaat ggcatccgca tagtgcactg ggttatcgct
     1021 cgccacggga atatctacgg cagcaagcca gtaatgggtt aagtgataac aggtgtctgg
     1081 aaatataggg gcaaatccat gcattgtgtg ctgcttgcgc acaataagct aatgcgaacg
     1141 tatagtaacc acattagact ttatgcgtct aaatgaggtt aatgatgacg cacagcacaa
     1201 ttaagcaatt gacaataaaa gcaattctcg aatacatcga agacaatatt gaggttaaaa
     1261 gtatcgatat tgaatcgata gtctgttttt ctggttacag taggagatat ttgcaatctc
     1321 tgtttaagtc tcacatggga gtacccatag ggaggtatat acaactcaga agagtttcaa
     1381 gggctgcggt attattacga ttgacatcat tatcattggc cagtattgca ggcaaactct
     1441 gttatgattc tcaacaaaca tttacaagag agtttaaaaa aaactcaggt tttacgcctt
     1501 tacaatatag aaacagtaat ctatggacct ttaaaaatct taccggtcca aaagaaccat
     1561 caattgtcat acccaaaccg aaactttgtt atcttgatga aaataaattt aaag
//
LOCUS       DAPHDX010000295         1596 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPHDX010000295 DAPHDX010000000
VERSION     DAPHDX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.299"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(182..835)
                     /gene="ribB"
                     /locus_tag="RH058_004560"
     CDS             complement(182..835)
                     /gene="ribB"
                     /locus_tag="RH058_004560"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV3139994.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(939..1087)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1209..1499
                     /gene="ubiK"
                     /locus_tag="RH058_004561"
     CDS             1209..1499
                     /gene="ubiK"
                     /locus_tag="RH058_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV3139995.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          454 a          407 c          355 g          380 t
ORIGIN      
        1 gtcgcataat aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat
       61 taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagc ggggcaaaat
      121 aaaaaccgaa gccagctaca acttcggttt cttgattaag gcagtaatta agcagcggtt
      181 ttcagctggc tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga
      241 gcgccatatt gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat
      301 cgtcattagt cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcaaat
      361 caatagtcgc ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg
      421 ggaaaacgtg gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc
      481 gaacggtcgt aatacgatca gcggcagaaa caccggtagt cacaccttca gccgcttcaa
      541 tggtcacggt aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat
      601 caagttgttt acggcgatct tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa
      661 tggtcagcgc catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt
      721 tttcacggtc ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg
      781 cattttcaac acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa
      841 aaaaacctca ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat
      901 agccaaaata acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac
      961 tctaaccgtc ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc
     1021 gctcgcgggc tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag
     1081 aataagcgga ttcactataa cgctaatgat tagcggcagc aacgcatagc ttcacataat
     1141 tctggtttat gacttaccct tatcgcacta caatggtact caacacctat cagtacaggg
     1201 aaactacaat gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa
     1261 tgcctaaagg aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac
     1321 aagctcagct gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag
     1381 tcctgttacg gactcgtgag aaactggcgc tgcttgaaca aagaatgagc gaactggaaa
     1441 atcgcagtac tgagattaaa aagcagccag atccagagac tctcccccca acgctgtaaa
     1501 catttgtctt taatgcactg taaattatga attaatcctc taatttacag tgctttctat
     1561 taccttatcg cttaattcca ttcagaatat catcac
//
LOCUS       DAPHDX010000296         1586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPHDX010000296 DAPHDX010000000
VERSION     DAPHDX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.300"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..602
                     /gene="tetR(B)"
                     /locus_tag="RH058_004562"
     CDS             3..602
                     /gene="tetR(B)"
                     /locus_tag="RH058_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV3139996.1"
                     /translation="MINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALL
                     DALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQY
                     ETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPP
                     LLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(580..1266)
                     /locus_tag="RH058_004563"
     CDS             complement(580..1266)
                     /locus_tag="RH058_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV3139997.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1274..>1586)
                     /locus_tag="RH058_004564"
     CDS             complement(1274..>1586)
                     /locus_tag="RH058_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HDV3139998.1"
                     /translation="GIGLEGGGIFTVGDECHAHFLVEQGKEAKIALEQAGLLVLAIRT
                     PLIRKLKQEKPGELGEIARVLAENNINILVQYSDHANQLILITDNDSMAASVTLPWAI
                     K"
BASE COUNT          475 a          353 c          307 g          451 t
ORIGIN      
        1 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
       61 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
      121 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
      181 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
      241 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
      301 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
      361 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
      421 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
      481 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
      541 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctt
      601 aaaagcagca taaccttttt ccgtgatggt aacttcacgg taaccaagat gtcgagttaa
      661 ccacccttta gattcataaa gcgaaaataa tgcggctcca acgtacccac ctaaatggaa
      721 acggcgttca ctccaatcta aacacgcaca acagatttta cgtgaatgtt tggaaggaac
      781 gtcaattccc atttcatgaa aatattgaat accacttaat gtgatcattg aaccattttc
      841 agtgatccat tgctgttgac aaagggaatc atagatctta acggcaactt cgccagctaa
      901 atgatcatag caagtacgtg cttttcgtaa atgcactggc gtggaaactt tggcatgtac
      961 gccatggttt aaggagatcc ccatcatact ttccatcaat tcagcaatat cttttcctgc
     1021 tagccgaaaa taacgatgct tgccttgagc tactactgtg attagctggc aatctaataa
     1081 tttagataaa tgactgctcg ccgttgaagc tgatatattc gccacagaac ttagctcagt
     1141 ggccgtccaa gctcgcccat ccatcaaagc actgagtatt ttaactcgtg aaatgtcaga
     1201 catagccgcc cctatcgcgg ctattgagga ctcaaaggta acctcttttc gtattaaatt
     1261 agccatcgca agttcacttt attgcccaag ggagcgtaac agatgcagcc atactatcat
     1321 tgtccgttat taatatcagt tggttagcat ggtcactgta ttgcactaaa atattaatgt
     1381 tattctccgc caatactcgt gctatttcgc caagttcccc cggtttttcc tgttttaact
     1441 tacgaattaa tggtgtccgg atcgcaagta ctaacagtcc agcttgctct agcgctattt
     1501 tagcttcctt tccttgttca acaagaaaat gagcatggca ttcatcacca accgtaaata
     1561 tccctccccc ttccaatcca atacct
//
LOCUS       DAPHDX010000297         1543 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPHDX010000297 DAPHDX010000000
VERSION     DAPHDX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.301"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            336..>1543
                     /locus_tag="RH058_004565"
     CDS             336..>1543
                     /locus_tag="RH058_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3139999.1"
                     /translation="MTDSNVLFFRKKIDINAELNLKEFLRFCRYDLTALGSDFNWESY
                     EWRGVAVFTKINLKRKVKTDKEQLLDEEYIDFAKAYMRYQSALTKSRWKLSASLTILK
                     VVEQTLLMVNKNANITGVSIPVLDEAIQTIKSLYKEHPAYDYGLELQKFVKFLGENGL
                     LIAGVLSWVSPIKPPEPVNRVSKKADEARLSKLPDETALNAMAEIFSLPDEALSCRDI
                     FTTSIFALLMCAPSRISEILALPCDCEITETDSNGVERYGLRFYSLKGYGADIKWIPD
                     VMVPVARRAIERLKKLSKNAREFAQWVEENPSTFFRRDSLPETGENEILKPWQICEGL
                     GYNLERKNGNVKFLSGVSFYNSQKKYTMNSLWEIILSRLPEGFPWYDKNKGIKYSNAL
                     CLLNIHQLEK"
BASE COUNT          519 a          271 c          341 g          412 t
ORIGIN      
        1 ggtgaagtaa aaagaaaaga agatattgaa tatgacataa ctccagatat aaaaattaaa
       61 aatcatgatg gcgaagacac tggcagttgt aagcaatgtt ccacatgttc agctaatgtc
      121 cccattcctt gttatacatg tatgcatttt accccctggc tggatggacc gcatgaaaag
      181 gtttatcagt atttgattgg cgaaagagaa cgaatataca gcataactaa cgacagcatt
      241 gtaacggaat cgctcgacag aacgatcatt gctgtaaagg aagtaattaa ccactgcaac
      301 ataatgcgaa gctcacacaa ggggaataaa tccatatgac cgatagtaat gtattattct
      361 tcaggaaaaa gattgatata aatgcagagt taaatctgaa agagtttctc cgattttgca
      421 gatacgatct taccgcttta gggagtgatt ttaactggga gtcttacgag tggagaggtg
      481 tcgctgtttt taccaaaata aatttaaaac gtaaggtaaa aacagataaa gaacaattgc
      541 tcgatgagga gtatatcgat tttgctaaag cttatatgag atatcagtcg gctttaacca
      601 aaagcagatg gaagctgtca gcttcattga ccatattaaa agttgtcgaa caaacattac
      661 tgatggtcaa taaaaatgca aatatcaccg gggtcagcat acctgttctt gatgaagcca
      721 tccaaaccat caagagtctt tacaaagaac atccggccta tgattatgga ctggaacttc
      781 agaaatttgt gaagttcctt ggtgaaaatg gtctattgat cgcgggtgtc ctttcatggg
      841 tcagcccaat aaaaccaccg gaacctgtta accgtgtgtc taaaaaagca gatgaagcaa
      901 ggttaagtaa acttccggat gaaacggctt tgaatgctat ggcagagatc ttctcactgc
      961 ctgatgaggc attgagttgc cgggatattt ttactacttc aatttttgcg ctcctgatgt
     1021 gtgctcccag tcggatatcg gagattttgg cacttccctg tgattgcgag attacggaaa
     1081 cagactctaa cggggtggaa aggtatgggc taaggtttta ctccctcaag ggttatggtg
     1141 cggacattaa atggattcca gacgttatgg ttcccgttgc caggagggca atagaaaggt
     1201 taaagaaact ttcaaaaaat gcccgtgaat tcgcacagtg ggtggaggag aatccatcta
     1261 cattcttcag acgggatagc cttcctgaaa caggagaaaa cgaaatatta aaaccctggc
     1321 agatatgtga aggattagga tataaccttg aaagaaaaaa tgggaacgta aagtttttat
     1381 ctggagtgag tttttataat agccagaaaa aatacaccat gaactcatta tgggaaatta
     1441 tcctctccag attaccagaa ggttttccat ggtatgataa aaataaaggc ataaaataca
     1501 gcaatgcact ttgcctgttg aacatccatc aactggagaa aag
//
LOCUS       DAPHDX010000298         1541 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPHDX010000298 DAPHDX010000000
VERSION     DAPHDX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.302"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            808..1410
                     /gene="fimB"
                     /locus_tag="RH058_004566"
     CDS             808..1410
                     /gene="fimB"
                     /locus_tag="RH058_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV3140000.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          462 a          263 c          333 g          483 t
ORIGIN      
        1 gtgagcttgg tgaaaatcag tgtttacccg ccatcaggct gagcataatt ctcatcatga
       61 aatatgtttc ctggtttgtg gcttgtaact ggtcacttct gaagtcgatc tggagaggct
      121 tgttgatgtt ggtgttttca ggatgatgtt tcacttagtt tgtttgccgt atcgcccggc
      181 gaatggctgt gattgaggaa ggtttaagtc gtagtgacca aagctatatt taccaacgaa
      241 tgtagatgaa aaaatcatct cctgcgttcc cccatatctc caggataaaa aggaatgtaa
      301 caatctcatg gcgtaagctg acgaatcagc caggaataat cgctagggac ctaagaatta
      361 gcatgataat agccactaag aaattactgc gctccatgaa atagccattt tgtggcaaat
      421 ggagttgact aataatgtca tatgtgagac ggctagttga acgaatatta aattttgctg
      481 aattttttat gttgatttta cttgttacag aacatatcac atgatatata gataagatta
      541 gttgcattaa tgatgagggt tattattaga ttcgtatccg attgataaat atataaaggt
      601 acatagcatg caagagcatg gcgtttgtat ggcaacgtta ttataattaa ctgttgctac
      661 tccatttaag ttcactcaga agaactggtc cacttacgtt agttattaag caaacgttcg
      721 cttttataaa cataatcagg ataaaaatgt tggattattg ctaacccagc acagctagtg
      781 cgcgtctgta attataaggg aaaaacgatg aagaataagg ctgataacaa aaaaaggaac
      841 ttcctgaccc atagtgaaat cgaatcactc cttaaagcag caaataccgg gcctcatgca
      901 gcacgtaatt attgtctgac tttgctttgt tttattcatg gtttccgggc gagtgaaatt
      961 tgtcgattga ggatttcgga tattgatctt aaggcaaagt gtatatatat ccatcgatta
     1021 aaaaaaggct tttcaacaac gcatccgcta ttgaacaaag aagttcaggc tttaaaaaac
     1081 tggttgagta tccgtacttc gtacccgcat gctgagagcg agtgggtatt tttatctcgt
     1141 aagggaaatc cgctttctcg gcaacagttt taccatatta tctcgacttc cggtggtaat
     1201 gccgggttgt cactggagat tcatccgcac atgttacgcc attcgtgtgg ttttgctttg
     1261 gcgaatatgg gaatagatac gcgacttatc caggattatc ttgggcatcg caatattcgt
     1321 catactgtct ggtataccgc cagcaatgca gggcgttttt acggcatctg ggatagagcc
     1381 agaggacgac agcgtcacgc tgttttatag agatagttga gataccaggg atggtgttaa
     1441 aaactacatt attcttacga tatatcaaaa atgattaacc atttattaat tgatgttatt
     1501 gtaagtgtga ttattaattt tcataataaa cattaagtta a
//
LOCUS       DAPHDX010000299         1509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPHDX010000299 DAPHDX010000000
VERSION     DAPHDX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.303"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..1246
                     /locus_tag="RH058_004567"
     CDS             53..1246
                     /locus_tag="RH058_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3140001.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
BASE COUNT          312 a          379 c          429 g          389 t
ORIGIN      
        1 aaaattaatt ttacaaataa acacaattaa tacatggtgt tgaaacatta caatgtcatc
       61 taagatcgaa gatacgcccc aaaaaaccct gtcctgctgg ccactggcgt tcagtgccgg
      121 tcttctcggt atcggacaga acggtctgct ggttgtactc cctgttctgg tcatacagac
      181 aaatctgagt ctgtctgtat gggctgccct gctgatgctg ggctcaatgc tgtttctgcc
      241 atcttcccca tggtggggaa agcaaatttc ccttactggc agtaagactg tggtgctgtg
      301 ggctctggga ggatatggcg taagctttac cctgcttggg ctgggaagcg tgctgatggc
      361 taccggtgcc gtaacaacag cggtggggtt gggaatatta atcatcgccc ggatcgtcta
      421 cggtctgacc gtgtcagcaa tggtgccagc ctgtcaggtc tgggcattgc agagagcggg
      481 agaagggaat cgcatggccg ctctggcaac catcagctcc ggcctgagct gcggcaggct
      541 attcgggccg ctgtgcgcgg cggcaatgtt ggtcattcac cctctggcgc cagtgtggat
      601 gctgatggca gctccggtgt gggctgtggt gatgcttctg cggttgcccg gcacaccacc
      661 acagcccaca ccggagcgca agagcgtcag cctgaagcgg gattgcctgc cttatctgct
      721 ttgcgcaatg ttactggctg cggcaatgag catgatgcag cttggacttt cgccagccct
      781 tactcgccag ttcgatactg ataccaccac tattagccaa caggtggcgt ggttgttggg
      841 gctgtccgca atagctgcgc ttatcgcgca gttcgtggta ctccgtccac agcgcctgac
      901 tccagtggct ctgctcctga gtgccggggt gttgatgagt agtggtctgg ctatcatgct
      961 cactgaacag ctatggttgt tttacctggg ctgtgcagtg ctgtcatttg gggccgctct
     1021 ggcaacaccc gcttatcaac ttttactgaa tgataagctg gccgacggcg caggcgcggg
     1081 ctggcttgcg gccagtcaca cacttggcta tgggctgtgc gcattgttgg tgccactggc
     1141 gtcgaaaaca ggtgtctcaa tcgcgctgat tgtgacggca ttatttgccg ctgtattatt
     1201 taccatagtg tctgcatgta tttggcatta ccgtacaatc aagtaaggct atgatgttgt
     1261 actcacagcc ggcataacgt cctgtgcaat ttcgctgtcg aaaccgcctg tgctgaccgc
     1321 ccagtgcact cacctgaagc acatcgcagc atgccaccag gataccagat cgtcttctga
     1381 aatgggtgga tacattggca acgttaaccg caacaatttt tcagagagaa ttttgggggg
     1441 tgagggccag tgatgtaaaa aatacgccaa gaggttaatt cattgtttat actgatatgt
     1501 caggtaatg
//
LOCUS       DAPHDX010000300         1503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPHDX010000300 DAPHDX010000000
VERSION     DAPHDX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.304"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..726)
                     /locus_tag="RH058_004568"
     CDS             complement(<1..726)
                     /locus_tag="RH058_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDV3140002.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLK"
     gene            complement(767..982)
                     /gene="sctI"
                     /locus_tag="RH058_004569"
     CDS             complement(767..982)
                     /gene="sctI"
                     /locus_tag="RH058_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV3140003.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            complement(1074..1314)
                     /locus_tag="RH058_004570"
                     /pseudo
     CDS             complement(1074..1314)
                     /locus_tag="RH058_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1328..>1503)
                     /locus_tag="RH058_004571"
     CDS             complement(1328..>1503)
                     /locus_tag="RH058_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV3140004.1"
                     /translation="SELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNR
                     FEKSQGSNWTFQE"
BASE COUNT          445 a          302 c          247 g          509 t
ORIGIN      
        1 tttcaacctg actaaattag ttagaatggc cctatacttc cataacagcc agcaagtcgc
       61 tacggatatt aatgcaagta agatagaaac cggcatagcc ttatcataag caaaaacagg
      121 ttcgctaatt tcatatgttg gtgcttgctc aataatgtct cttcgttttg acaatacaac
      181 agaaatattt tcatattgta cgcttgcaga gctattaaca ataaatctct tgatatcatt
      241 tatttttatt tctgggttga tatctttttc atatactgca agtacagaaa tatgaattgg
      301 taaagcagtt ttaccactat cgccattatc aacatcgtaa ctaacatgta ctctcgaaga
      361 aataatgcca tccataattt tgagagattg ctctaaccgc tgctcaatag cagaatatag
      421 ccttgctttt tccgctcgtg gagatgaaac gagtgcatct gcagggaaca tctgcgatat
      481 ttgaatatca ggcttacccg gtagattgta gatttttagc caatccaccg cagaagcaaa
      541 atccgttggt tcgacaaata ttgaaaatcc tgttttgcct tgatccttct tttcagcatt
      601 aatattatgt ctttgtaaaa cagcaaggac atcattagct tgctgttgat caagatggtt
      661 caataattcc tgctgcttgc agccgcacaa cagcaggata aacaataata atgatatata
      721 tttcatgagc gggtcaacgt ctcaactgca gcgacaattt ttcttgctaa tgttccacca
      781 atgaaacctc gacattataa ttaagcacat gatcctgcaa gactaataat ttttcaggag
      841 ttaacccctc tttatcagac cttaatgctt gtaacgcatt ttgctgccaa ttttcagttt
      901 gtacagcata gttggcaaaa gcggagatca ttttatcatc cgggttgtca agattgagaa
      961 cccggtcatt taaatcaatc actttcgctt gctgtatttc atgagcagtt gattactcag
     1021 ctctatcaat aggtggcata ttgcttactg acataataaa atccttatcc agtttagcgg
     1081 aagttttgta tgattgctga atcaagatct ttatacgctt ttacaacgtt agattgcgca
     1141 tttcgatata atgtatactc agctaatgca ctttgatatt cggcaagaaa tttcgtgtcg
     1201 gagggattgg ttggctaaat cctccaacgc tttttcaact tgctggttca gatcatcaac
     1261 gccctgatca aattgattgc tgatatccat aatataacca ttccaatctg ccataagtcc
     1321 tcctcaatta ttcctgaaat gtccagttgc taccttgaga cttctcgaat cggttatctc
     1381 cgtagctaaa tgaactatta tttgtgtcct gatttttcaa attcacatta aatcttacat
     1441 attgtttacc ccattgttta taatattcgt caacatagtt attaatttta tttaattcac
     1501 tgt
//
LOCUS       DAPHDX010000301         1488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPHDX010000301 DAPHDX010000000
VERSION     DAPHDX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.305"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..303)
                     /locus_tag="RH058_004572"
     CDS             complement(<1..303)
                     /locus_tag="RH058_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HDV3140005.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNF"
     gene            1087..>1488
                     /locus_tag="RH058_004573"
     CDS             1087..>1488
                     /locus_tag="RH058_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDV3140006.1"
                     /translation="MSKKKISVENEEFITYRALLLSIDFVKKLVTEMSDEDLNKVKEN
                     FDIAIQQRDERKENMKAIKSLMDEHGISLEDFCREFGKKSQAGRKSVSGGHKYEYDDN
                     GKRMYWNGQGRRPKAIAEAIKAGGSLDDFLIS"
BASE COUNT          500 a          232 c          301 g          455 t
ORIGIN      
        1 gaagttgaag tttcctagaa accgtgacac tgtttcaggt tcttttacgg aaatatcaaa
       61 ctcgttgacg aggggatcat tttgaagtag ctttagacgt tctaacttat caatgccaat
      121 gaagtgaccg cagagcatgg tctttatatg attcatcttg attttatttg ttgagtcatt
      181 atcaaatacg aggtcatttt caataaaatc aaaaatccca ttgctttttg cattctcaag
      241 gagcagaaaa agacctgcat ttgatgttag attcttagct ttgaaatcaa ttttattaat
      301 cataattaga accccttttt actacttttc ttactattat tttaccatat atcgagtcat
      361 aaaagctgat aatttaacat atttttgagc acttttcttt cacccaatgg gtgaaagctg
      421 aatttcgaag gaatgcatat ttatcaaggc tttgattatg ctttttgaag tactgacgta
      481 gaatctaggg gtaatattaa tgacaagacc agcaggttct ttagacaaac gaacaatatc
      541 agcaaatacg acagttccag gagaggtaaa aagtttttta cttgcacaat tcttaaaaaa
      601 atggagacga acaaaaatgg ggcgacatac ctggaaagta tcgctaccag atgatgctaa
      661 aaagcagaaa tagtgttcca ggcctgaaag ggatgagaat cagtatctgt gtgtatttat
      721 aacatcttct tgaagaggct ggagattgtt agtccattag tataatcgat attcattttc
      781 ctgcgaatat gcaggctaca ccagatatat gattgctacc gaatttcatc agtttgatga
      841 aattacgcat catactgcgg gttttctcat taacattaac gaaagtgaat ggactcagac
      901 aagtagtgac cggcgcaatt tcgctaagta tcaaatacaa agaacgttct cttactttct
      961 gcgatggcct ttccttcgga aaggaagtga cgattaagtg tcgaaaaatg tgatacatat
     1021 attattttta tggcaaatca cctgatagga tttgccataa atgaatgaaa agctgagtgt
     1081 taataaatga gtaagaagaa aatttctgtt gaaaatgaag agttcattac ttaccgggca
     1141 ttacttctga gtattgactt tgttaaaaaa ctggtcacag aaatgtctga tgaagattta
     1201 aataaggtta aggaaaactt tgacatagct attcagcaac gtgatgagcg aaaggaaaat
     1261 atgaaagcta tcaaatcact catggatgaa catggtatat ctctggaaga tttttgtcgt
     1321 gaatttggca aaaaatcaca ggcaggcaga aaaagtgtgt caggagggca taaatacgag
     1381 tatgacgata acggtaaaag aatgtactgg aatgggcaag gacgtcggcc taaggccata
     1441 gcagaggcta taaaagccgg aggttctctt gatgattttc ttatatct
//
LOCUS       DAPHDX010000302         1476 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPHDX010000302 DAPHDX010000000
VERSION     DAPHDX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.306"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..265
                     /locus_tag="RH058_004574"
     CDS             <1..265
                     /locus_tag="RH058_004574"
                     /inference="COORDINATES: protein motif:HMM:NF013548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3140007.1"
                     /translation="TDVYDLKYYSSEETISDSRSDTDNNFAWSLGVGVKYNIYDNLSV
                     DLAYRYFDAGKSQITYRNEWNEEFKSRVSVRSNDIMLGVTYDF"
     gene            391..933
                     /locus_tag="RH058_004575"
                     /pseudo
     CDS             391..933
                     /locus_tag="RH058_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          440 a          259 c          360 g          417 t
ORIGIN      
        1 cacggatgtt tatgatttga aatattattc atcagaggag acaatttcag attcacgttc
       61 agatactgat aacaattttg catggagtct gggtgttggt gtaaaatata acatttatga
      121 taatctgagc gttgacctgg cataccgtta ttttgatgca ggtaaatccc agattactta
      181 cagaaatgaa tggaatgagg aatttaaatc cagagtttca gtcagaagta atgatattat
      241 gcttggcgtg acatatgatt tctgacatac catattgaaa tattacatta gtttatcaaa
      301 atgttataaa attaccggat gttctgggag tggaacatcc ggtttgtgcc taatgaggga
      361 attgtggcac tccgttactg ttccagtcga attgcggata atactcaact aatcagtcta
      421 acctgcggtt gttcacaatc accgacaccg taaatggcgt ccccctgata tctgccagtt
      481 catgacatta gtcgaagtcg gtgttgcgtt atgtgccagg agcatggttg tcgggagact
      541 gcctgtcccg tgggctggtt ccggcaaccg gtatactctg tggttcgaat tgttcgtgct
      601 ctcatggtta aaaatcagta ctgttgaagg cgtcaaaaac cagttggaat gctgttgacg
      661 gcattatgac acgtgcggtt aagtgcggcc tgtcccggat aatccgtatc ttcctggtga
      721 aagtcgttat caggaaaagg gaatttgctt ccggtggcag tacagcgata agtggatgac
      781 caaatccagg caggaaaagc tgatatggct gcgtacgcag atgaagctga cgagccattt
      841 cctggcgcga tgaaagaact ggcaaaggat atcaggagca gaccatgtat cgaggaaaga
      901 cgcaatgact ggcagagatg gatggcgttg gcggtcaaca tggaaaatcc atgaatgtga
      961 aatttattat tgtcatggtt ataatgacta tattaacggg gtatgcttta cctgccatac
     1021 ctgaacggtt aaagatatga ttgctgccgt tccggtttat cagttgaaat acagaatcct
     1081 attctataat gctctgagaa tgaaaacatc tgcgtataca attacagaat gtgcactgac
     1141 gcctgcggag gaaatattag aggacttgac atctcattat tatatccgcg ttatgagtga
     1201 aaccagaaag tatatggaca gttgattact gaagattaat aatcatttaa cccatttgct
     1261 gaagaaaaaa cttaatgact ttattttgat tacgataatt ctgacagagt tattagcata
     1321 aaactcaggt atcagcaaga tgagcaagga ggcatctgca gcaacaggtg ctacaggtgc
     1381 tacaggtgtt ccaggtaagg ataccgaaac ctgataatgg tcggtatggg gaattgcaga
     1441 gatttacggg catatgacct gaagaatgaa gtatgc
//
LOCUS       DAPHDX010000303         1472 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPHDX010000303 DAPHDX010000000
VERSION     DAPHDX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.307"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..96
                     /locus_tag="RH058_004576"
                     /pseudo
     CDS             1..96
                     /locus_tag="RH058_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            168..1172
                     /locus_tag="RH058_004577"
     CDS             168..1172
                     /locus_tag="RH058_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HDV3140008.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
BASE COUNT          422 a          323 c          346 g          381 t
ORIGIN      
        1 ttcaaagggc tgagctggga ttacaaaacc aactcaccct gtaaatttgg cagtaaaatt
       61 atcgtcaaca atctggtgag atgggaccag tggggataat gatatggtcc cccccacctt
      121 aacagcagta cgctgttaag gtaaatccct ttaacggaga gaccatcatg cagaacatca
      181 cacttattgg tatcgatctc ggaaagcact ccttccacat tcattgtcaa gacaagtctg
      241 gtaaggctct tctgcgtaaa aaatttaccc gtactaaact gatgcagttt ctggcgacat
      301 gtccgccctc agttgttgta atggaagcct gtgcgggtgc acactttatg gctcgctgta
      361 tctctgattt tgggcatgaa actaagctga tttctccaca gttcgttcgc ccgtttgtca
      421 aaagtaataa gaatgatttt gttgatgcag aagccatatg cgaagcggct tcacgaccat
      481 caatgcgttt tgtccagcca agaactgaga cccagcaggc catgagggcg ttacaccggg
      541 tcagagaatc gctcatcaga gataaggtga aaacaaccaa ccagatccac ggtttcttgc
      601 tggaatttgg aatcagcctg ccgataggtg atgctgtaat aaaacggttg tctctcgtgt
      661 tggctgaaca cgaaatcccc gaatatctga gccgcctttt ggtgaggttg catacccact
      721 acttttacct tgttgagcaa atagcagagc tggaatcaga attaagtcag tcaatcaacg
      781 ctgatgatac cgctcagcgc attatgacaa ttcctggtgt tggtccgatt actgccagct
      841 tactatcgtc gcaacttggt gacgggaaac agttctcatg cagcagggat tttgcagctt
      901 ccacaggact tgttccccgg cagtatagca ctggaggaaa atcgactctg ttgggtatca
      961 gtaagcgagg tgataaaaat ttacgccggt tactggttca gtgtgcccgc tcattcataa
     1021 tgcgacttga acgcaagcat ggaaaacttg ctgagtgggt tcgggagcaa ctaaacaaga
     1081 agcattcgaa tgtggttacc tgcgctctgg caaataaact ggctcgaatt gcctgggcaa
     1141 tcactactca gaaaaaagaa tatcaggcct gatatagatg ctgatataac tcaattagtt
     1201 tattttaaac aataagatac aagttacctt tctggttttg cgaagactga ttattgatga
     1261 catgaacggc aaatcgacct gattaaaaac ctaacagatg aaatggctca ctgaagccga
     1321 ggggattttt aggattatca ggtgcggaac tcatcggggc gcgggcatcc ttgcccacag
     1381 agacgccgga tagatttaag caagccaacc attaatcaaa aatcagtgtt gcaaaaacgg
     1441 gggtgaccat agatttcatc ttatcagtgg aa
//
LOCUS       DAPHDX010000304         1454 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPHDX010000304 DAPHDX010000000
VERSION     DAPHDX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.308"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..827
                     /locus_tag="RH058_004578"
     CDS             117..827
                     /locus_tag="RH058_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3140009.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            869..>1454
                     /locus_tag="RH058_004579"
     CDS             869..>1454
                     /locus_tag="RH058_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HDV3140010.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNG"
BASE COUNT          418 a          333 c          304 g          399 t
ORIGIN      
        1 tacaaacgca ttaagtctgg aaacccggcg actcgccaat gtattagcaa cattaatcga
       61 ttgtctgatg taacggtttt cccgcttatc ctcttaacac atggagaatg catcagatgc
      121 aaactacgcg taccccctac tctatttcct ttatggcgac agtgttactt ttattgctct
      181 ttgcttgcca tagcaccgtt gccaacgcag ctgtcgcact gggtgcgacg cgggtaattt
      241 atccggcaaa ccagaaacag gttttattgc ctgtcacgaa taatgatcct gccagtgtct
      301 atctcattca gtcatggata gaaaatgcag gtgaccaaaa agacacccag tttgtcatta
      361 ccccaccgct tttttccatg caaggtaaaa aagagaatac gctgcggata atcaacgcta
      421 ctaatcatca gttaccaggc gaccgtgaaa gcctgttctg ggtgaatgtt aaagctattc
      481 ctgctatgga aaaagaccag aagaatgaga atacattaca gctggcaatt attagccgca
      541 taaaaatgtt ttatcgcccc acgaacctgg caatggcacc tgaagaagcg cctgcaatgc
      601 ttcggtttcg tcgcagcggt agcaaactca cgcttattaa tccaacgccc tatttcatta
      661 ccgtaacaaa tatgaaagca ggaaatagca atctcccaaa caccatggtg ccgccaaaag
      721 gtgaagtatc ggtggatatc acgcatgctg caaccgggga tatcagtttt cagacgatca
      781 atgattacgg tgcattaact ccgcgtatta aggcaaccat gcagtaagcc agtgacctaa
      841 ttaataataa actcaatcgg aattaataat gacagccatt cgtgccgcat ttaaggcata
      901 tcgtatgcat caggtgttaa taatgcctcg ctttgcacgg cttaccattg cccttggctt
      961 agcaactgcg gtatttcccg ttgacgcaga gtattatttt aatccacggt ttttaagcaa
     1021 tgatcttgct gaatctgtcg atttatccgc ctttactaag ggacgcgagg caccacctgg
     1081 tacatatcgg gtcgacattt atttgaacga tgaatttatg actagtcggg atattacttt
     1141 tattgcagat gataataatg cagaactcat cccatgcctg agtacagatc ttcttgtcag
     1201 tcttggtata aaaaaatcag cgttattaga caataaagaa cattcggcag aaaaacatgt
     1261 gccggacaac agcgcatgca caccacttca ggatcgtttg gccgacgcat caactgagtt
     1321 tgatgttggt cagcaacatt tgtcactctc cgttccgcaa atttatgttg gcagaatggc
     1381 tcgcggctat gtttctcctg atctgtggga agaaggaata aatgctgggc ttctaaacta
     1441 tagttttaat ggta
//
LOCUS       DAPHDX010000305         1433 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPHDX010000305 DAPHDX010000000
VERSION     DAPHDX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.309"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   38..1347
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=38..66
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
BASE COUNT          322 a          427 c          424 g          260 t
ORIGIN      
        1 aaccgccagg gtgagccagc gccgcaggaa gacacgcgtg ttccccgcgc cagcggggat
       61 aaaccgcgtt cccaacgctg aatattgctg gcatagccgt gttccccgcg ccagcgggga
      121 taaaccgcca atacggagaa ccttcatcgg taatggggtg tgttccccgc gccagcgggg
      181 ataaaccgga gcgtagagat tctcgaaacc gatccggacg gtgttccccg cgccagcggg
      241 gataaaccgg aagttgcgcg cactaagaaa aacaggttcg agtgttcccc gcgccagcgg
      301 ggataaaccg ctgaaatcaa tcgccggtcg tcgccacgtt acgtgttccc cgcgccagcg
      361 gggataaacc gtcatcgaaa accagcggat cgaatgggac gccgtgttcc ccgcgccagc
      421 ggggataaac cgagacggcg cgtaaaactc atacggcgac aaatgtgttc cccgcgccag
      481 cggggataaa ccgctcgcgg gcgtggaatt taacgaacgg ttaccgtgtt ccccgcgcca
      541 gcggggataa accgtcggga cgctgcgcga tctgcatagc aacgcagtgt tccccgcgcc
      601 agcggggata aaccggtatt tttgctgacg gattctcaga gagtttcgtg ttccccgcgc
      661 cagcggggat aaaccgtttc tatctcccag tgggagagag atgacagtgt gttccccgcg
      721 ccagcgggga taaaccggtt ttggcgatat cacctgatgc ctgcaatccg tgttccccgc
      781 gccagcgggg ataaaccggg gtatcgcact gcggcagatg ttccggggcc gtgttccccg
      841 cgccagcggg gataaaccgc atgaatatgg acgatgaaaa aataagagag ggtgttcccc
      901 gcgccagcgg ggataaaccg ttaaaacatt ttccgagctt tgcgatttat atgtgttccc
      961 cgcgccagcg gggataaacc gtaaaaatca tcatggaaaa tcaacccggc gacgtgttcc
     1021 ccgcgccagc ggggataaac cgagcgcgcg cgggctactg cactcggtga taacgtgttc
     1081 cccgcgccag cggggataaa ccggcgatct cgcggaatac accgacgagg cgggcgtgtt
     1141 ccccgcgcca gcggggataa accgtaaggc cgtcgccgga tcagcctggc tatgccgagc
     1201 tccccgcgcc agcggggata aaccgttctt gcgggtgttg caaatattct tcacgtagag
     1261 ctccccgcgc cagcggggat aaaccggacg ccgccgccgc gaagccgttt ccgatgttga
     1321 gctccccgcg ccagcgggga taaaccacca tataacccgt tatctctttc tcaagttttt
     1381 atattagcag tacttgtaat aagcaacata tccacgtaac acctcatgtt caa
//
LOCUS       DAPHDX010000306         1417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPHDX010000306 DAPHDX010000000
VERSION     DAPHDX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.311"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..373)
                     /locus_tag="RH058_004580"
     CDS             complement(<1..373)
                     /locus_tag="RH058_004580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140011.1"
                     /translation="MIAFDTQRLRDFMAGLVVFIICVILAVLTFRKVSNYSRNKGRGK
                     FRTLITSLGMSFIIFVVSVGIGSGVVSQDKSTDVKASTSTSTEKAKTNVYKCDKFDAI
                     IQTRQGTKSDSGYYSDKGILIY"
     gene            907..>1417
                     /locus_tag="RH058_004581"
     CDS             907..>1417
                     /locus_tag="RH058_004581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140012.1"
                     /translation="MDENNKEVIEIRPEDKDFFAEFIDCEGPIIQDSIDHKKITLALD
                     KAHDIRKFEIDLYWKRATYFFAFFTVITAAFGYLFTHDNYTFLAPALAIIGSVFALCF
                     CYVNIGSKYWQENWEFIIDKIEYYVTGNLYKLFFFENHRTKRPSVTKINIFLSKLIIA
                     TWYACFILSM"
BASE COUNT          435 a          275 c          266 g          441 t
ORIGIN      
        1 aataaattaa aattccttta tctgaatagt aacctgaatc gctttttgtt ccctgtcgag
       61 tttgtattat tgcatcgaac ttatcgcatt tataaacatt ggttttggcc ttttcagtac
      121 tggtagacgt tgaagccttt acatcagtac ttttatcttg tgaaacaacc ccggaaccga
      181 tacctaccga aacgacaaag ataataaaac tcatgcctaa cgacgtaatc aaagtacgga
      241 actttccccg acctttattt ctggaatagt ttgaaacttt cctgaaagta agtactgcaa
      301 ggatcacaca aatgatgaaa acgacaagcc cggccataaa atccctcaaa cgttgtgtat
      361 caaaggcaat catagtatca cttcgcaata cattgatcga ttaaagcgat caatttttga
      421 ttttcgatcg gttaaatcta tcaatatcta ttaattgaag acaaatcaat aggtaaaaac
      481 gatcagacgt tggtcagtac ttcggaaaca ccagaagtat ggtttgggtc tgccgtttaa
      541 acgccctcag aaaaccgcta aggtctcttt atcgcatatg gcttgggatt tcagcctttg
      601 gctaaaatcg ctttcagggg ggcttcttgt gcgttttgtc gggaacacca tcatggaggg
      661 ggataaatcg gatcgaagtc gcttcgctct ggtcagtact ggaagggtta gcttatctgg
      721 cgagggaaga tgaattgcca ggcttcatga agatggttgg ttttgtggac aagaagaact
      781 gccgttttga gtattccccc tcacacccaa caaacctacc cattagcaag ggggagtact
      841 ttctcgtagt atacagaacg aataaattct ttgtatactt ccgttttatc ctaaagcgat
      901 ttgattatgg acgagaataa taaagaagtt attgaaataa gaccagaaga taaggacttt
      961 ttcgcagaat ttattgattg tgaaggtcca attatccaag attcaattga tcataaaaaa
     1021 atcaccctcg cacttgataa agcccacgac ataagaaagt ttgaaataga tctctattgg
     1081 aagcgtgcta catacttctt cgcattcttc actgtaataa cagctgcatt tggttatttg
     1141 ttcacccatg acaactatac tttcttagcc cctgcacttg cgatcattgg ttctgttttt
     1201 gctttatgtt tttgttatgt gaatatcggt agtaagtatt ggcaagaaaa ctgggagttt
     1261 ataattgata aaattgaata ttatgtcaca ggaaaccttt ataaactttt cttcttcgaa
     1321 aaccatagaa ccaaacgccc atcagtcact aagattaata tatttctaag caagctaatt
     1381 atagctacat ggtacgcttg ctttatactt tccatgc
//
LOCUS       DAPHDX010000307         1410 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPHDX010000307 DAPHDX010000000
VERSION     DAPHDX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.312"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..491)
                     /locus_tag="RH058_004582"
     CDS             complement(<1..491)
                     /locus_tag="RH058_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140013.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDL"
     gene            complement(513..1052)
                     /locus_tag="RH058_004583"
     CDS             complement(513..1052)
                     /locus_tag="RH058_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140014.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1308..>1410)
                     /locus_tag="RH058_004584"
     CDS             complement(1308..>1410)
                     /locus_tag="RH058_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV3140015.1"
                     /translation="KISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          431 a          251 c          261 g          467 t
ORIGIN      
        1 tctaaatcga cattacgtaa gtccagagag gatagatcaa cgatatctcc agttccacaa
       61 ctaaagatta cgttcaacct cctgatttca ttgtaaatat atttcaatgg acaattaaca
      121 ttgatactgc ctgtggatat tttctggttc tttaatggtt cattaatggt aagttcgcag
      181 gtattgtccg atcgttgaat aatttgggcg ttataaccta aaaaatccag acgaagagac
      241 gagtcattat aaagaattac attttgtaag tacgctaatt ctttttgaaa atcaccagtg
      301 ataatgcatt ttccaccagg ttgtaacgtg ttaagtaaag tgaccatttc aatagaggtc
      361 tgttttagtt tttctatacc tggtgctagt gcttcgaggt tatttcctgt tgggctttca
      421 ggaatgtcat tcatttttcg acggactaaa tcactactaa tatcattacc aattgatgaa
      481 acagggaaca ttattttatc ctttataaat aatcaataaa aatcattatt aaaatgatcg
      541 ggtgaaaaaa agagaatttg ccaacaatct gaagtaatgc tggttttatt aaagtgcaca
      601 aggggtgtta agatggcttc ttttcgggtg aattttgcac aatacttaga aatcgtctcc
      661 ttaatacatt ccagctcttt tttattgacg gctgagcaag tacattgttc gccaaaaggt
      721 gcaaaagtgc ctacatctaa tgtttgtatg atataatcat catgttcccc tgtagaaata
      781 tctatgatga atggactcat attttccggt tggattcgga tcattacaga aggaacatcg
      841 ccttgataga aattcaatgc agtaattgca gataatactc tccgcagttc attacttata
      901 ctgtaaattt tattaacgtc attggtggag acaatttctg caaactttct ttttaccgtc
      961 caataatcaa tatttctatt gtgattgtta accactgcgt gcattggcgg gattgaaggg
     1021 tgaaaaatat gtgcaattgg gaagcctttc attgatgcct gccttgtcgt catgacaaac
     1081 aataatagat gcttattttc ctcgcagggt tctttcgcac tatcaaaaaa cgctcattta
     1141 cccggcgtta cagtaaacga agcaacattc tgtggcctac gacgatcaaa agctctgtga
     1201 aaatttccct tgttgtcttt gtctgacctt atcgcggcga ccagcaatta ttcgttttgt
     1261 gatcctcaac acaaaatgct attcgtcgcc tatgagggga gaagaattta tggaaaacga
     1321 ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt tgacgttaat caattgttcg
     1381 agatgatcaa tacctgtatt cgaaatttta
//
LOCUS       DAPHDX010000308         1405 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPHDX010000308 DAPHDX010000000
VERSION     DAPHDX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.313"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            138..542
                     /gene="stpA"
                     /locus_tag="RH058_004585"
     CDS             138..542
                     /gene="stpA"
                     /locus_tag="RH058_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV3140016.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(589..1113)
                     /gene="ygaP"
                     /locus_tag="RH058_004586"
     CDS             complement(589..1113)
                     /gene="ygaP"
                     /locus_tag="RH058_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV3140017.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1123..>1405)
                     /locus_tag="RH058_004587"
     CDS             complement(1123..>1405)
                     /locus_tag="RH058_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3140018.1"
                     /translation="LQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGL
                     SASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          382 a          331 c          334 g          358 t
ORIGIN      
        1 agtttcagaa aataacagct gaaataatct cgcgcaggac tgtaaataga ttaaattttg
       61 tggaaatata ataagtgatc gcttacacta cgcgacgaaa tacttttttg ttttggcgtt
      121 aaaaggtttt ctttattatg tccgtaatgt tacaaagttt aaataacatt cgcaccctcc
      181 gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa aaattcaggg
      241 ttgtcactaa agaaagacgt gaagaagaag aacagcagca gcgtgaactg gctgagcgtc
      301 aggaaaaaat tagcacctgg ctggagctga tgaaggctga cggaattaac ccggaagagt
      361 tattgggtaa tagctctgct gctgcaccac gcgctggtaa aaaacgccag ccgcgtccgg
      421 cgaaatataa attcaccgat gttaacggtg aaactaaaac ctggaccggt cagggccgta
      481 caccgaagcc aattgctcag gcgctggcag aaggtaaatc tctcgacgat ttcctgatct
      541 aattactgag gccggatgtc gctgctaggg cgcgtccggc gtcgcttctc aagctcgttg
      601 gttccacggc atcttatcta acaaccttgc cattccacaa aaaccgctga ttcctgcaaa
      661 cagtaacccg gcaccaacaa agccacttaa taagaagaaa ccgctattta cggtataacc
      721 cagtacaacg ccgattaata ttaaaccacc cgcagcgatc tgcacctggc gcattaacgg
      781 caagggttga gatttattta ccgctactgg caatccagct cttttccagc catcaatccc
      841 atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct gctaatttat cggcgttatt
      901 actggtgcgt ttacctgcct ggcagtggaa tataatttgc tcgcgacgta atttaggtgg
      961 aagacctgac tgttccagca cggataatgg agccagatct gcttcaggaa tatgttcacg
     1021 aagatattca tcagcatcac gaatatcgat taactttgcg ccgcgtgcga ttaattcttg
     1081 tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact ccttacggac aatagacatt
     1141 tttcagggtg gcgataatgg catttaccgc ctcattttta atggaatata gaatgcgttg
     1201 ggcatcccgt tggctgtcga taagcccttc gtcccgcata cgagcgagat gctgtgatgt
     1261 cgcagaggca ctcagtccgg taatgcgcgt cagctctccc gcgctggtgc cgggggaacc
     1321 gctaagcatg cacagaatca gcaatcgttt aggatggctc attgctttca ataaggccgc
     1381 tgcctgttcg gcactggcct gtaat
//
LOCUS       DAPHDX010000309         1402 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPHDX010000309 DAPHDX010000000
VERSION     DAPHDX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.314"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(137..865)
                     /locus_tag="RH058_004588"
     CDS             complement(137..865)
                     /locus_tag="RH058_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3140019.1"
                     /translation="MKKIIAVSALAVAGMFSAQTLANESKTGFYMTGKAGTSVLSLSN
                     QRFVEGEGEWADRYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NLDKDDWSRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTSISTW
                     DYGYGSSGRKSLSRSGSADNFAWSLGAGVRYDVAPDIALDLSYRYIDAGDSSVSYKDE
                     WGDKYKSEVDVKSHDIMLGMTYNF"
BASE COUNT          420 a          302 c          296 g          384 t
ORIGIN      
        1 atcagaaatt atctgtaaaa ccgataacag atacccctgc agtctattac tgcaggggta
       61 tttctgttta attgcagaag atatgcaatt atcttaatta cagaatatac gcaattatcg
      121 ttcaggagaa atgtcgtcag aagttataag tcataccaag catgatgtca tgacttttaa
      181 catcaacttc tgatttatat ttatcgcccc actcgtcctt gtaactcaca ctgctgtcac
      241 ccgcatcaat atagcgatag ctgaggtcca gagcgatatc tggggctacg tcatagcgga
      301 cacccgcgcc aaggctccat gcgaagttgt cagcagatcc tgaacgtgac aacgatttgc
      361 gaccactgct tccgtaccca taatcccagg tactgatact ggttgttttc tggtgaattc
      421 tggcgtagcc aatccctgca gatacccatg gtgtgaatgc actgtcattc cggaagtcat
      481 agtacgcatt cagcataaga gtgttgactg atacctcatt cttcaggtca tcacgcgacc
      541 agtcgtcttt atcaaggtta tacttcgaat cagcttttcc acgcgcgtaa aactccagtt
      601 ccgtacgaac cggaatactg aattgcggat aaaaatcata accggccgca ataccgccac
      661 tgaataccgt atcatggtca tcgccacctt tgtatctgtc cgcccactct ccttcacctt
      721 cgacaaaacg ctggttggaa agagataaaa cggaagttcc cgccttaccg gtcatataaa
      781 agcctgtttt gctctcatta gccagggtct gggctgaaaa catgcctgcc acagcaagtg
      841 ccgaaaccgc aataatcttt ttcatttcag ttacctctgc tatcactttc cccgaacaat
      901 aaatggggtg cttattttaa aaccatgtaa aagacagggt aaaaagagaa aattcgatcg
      961 aatcgatcga caaaatcgat catgtatgat gaaagttcct tatcgtatca ataaaaatgg
     1021 taaggacggg tttaagagat aatcgcagca acatggatgt gttatgctca gaaagcacga
     1081 gaggttggga ttacaagagc atccccctga agacagtaaa gcaactggac gtctgatgag
     1141 ccgcatggaa tgtaattcag tgcgggtggc ttatatccat aaggcggagc atttagatga
     1201 gcgtaagttg atgttgctgt agtgggcgga ttatctagaa gtagtaagga aaaaaccatt
     1261 acatcttttg attacgcaaa gactaatgat tttggaatgt aaattttgct attggtgtaa
     1321 aaagcattta aaaacaagaa atttccatgt tttctaatgt ccggtgaacg tttggttgat
     1381 agggtagtaa aactagtaat ca
//
LOCUS       DAPHDX010000310         1401 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPHDX010000310 DAPHDX010000000
VERSION     DAPHDX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.315"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..136)
                     /locus_tag="RH058_004589"
     CDS             complement(<1..136)
                     /locus_tag="RH058_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3140020.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     C"
     gene            complement(331..1308)
                     /gene="wzzB"
                     /locus_tag="RH058_004590"
     CDS             complement(331..1308)
                     /gene="wzzB"
                     /locus_tag="RH058_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV3140021.1"
                     /translation="MRVENNNVSGQNLEPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYDSAAPKVSELQSILIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGQSPEAAQKQLAQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKELRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSDALESMVKHEASRPLVFSSTYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT          354 a          349 c          291 g          407 t
ORIGIN      
        1 cacacagctt tctcaaaata ctgcctattg ctctacgctt ctctctgtag aacacctgtc
       61 ttcggtattt tggcgcaaaa actatgtgat atttacagtt ccatcgggtg tgcgctaagc
      121 tcttttcgtt ccccattggg accccctttt gatttcttgt ttgacacttg cagttgccag
      181 accgcaaggt gttttaacaa atcaaaaggg gttttaataa ctggctcaaa gctgaaagct
      241 ttccggaacc cccagcctag ctgggggttt tctgtgcaca aaaaacgggc acggtgtgac
      301 caccctgtcc gctttcttta agctcaaaaa tcacttcgcg ttgtaattac gcagcgcgtt
      361 acgccccagt acaatacccg cgccaaccat gccgcccagc agcaccgcca gaatcaaggt
      421 gattgccttc ttcgggctat cacgacgaat gggtaacgta ggtttcatca catagcggta
      481 agcgtgaata tcaagatcat caacctttaa gctttcgata tccagcagat tttgacgagt
      541 ctgatagtag gttgacgaga acaccaacgg acggctcgct tcgtgcttaa ccatcgactc
      601 cagtgcatca ctccccaaaa ggaacatggt atcctgggtc acgtcctgtg tctgctgaat
      661 ctgcggcttt gtcacctgtg cctgattcgc atactgcaac gcttcctgaa tttgacgaat
      721 acgcagctct ttttgctcct gagcgaccac ttcctgggtc ttcagagaat cctgcaagtt
      781 tttcatacgc agcgcgatgt tgtctttcag atctttttca agttcttcat ttacctggtc
      841 gtcaacttgc tggatatatt gagccagctg tttctgcgca gcttcaggcg attgcccaac
      901 ataagacacc gctaaaggta atgactgatt tttaacagta ggttcaatgg tcagtttttc
      961 aggttcttcc tgattatcca gcgtttctgc taatgcagaa aatgtcgtgc tgaagcgacc
     1021 aattaaaatc gactggagct cagacacttt cggcgcggct gaatcataaa tcacatttat
     1081 ggcattgtta taaccagcga tttgccccac atcaggttga gtaacgatag ctgtggaagt
     1141 ccatttttct ttggcaacga ccagatatcc tattgccagg gcaatagcga caatgacaga
     1201 aatgatgatt gtcatcttgc cacgccatag ttgcaccagt aaatcaatca aatcaatctg
     1261 ttccggctca aggttttgcc cagaaacatt attattttct actctcatca ttaccctaac
     1321 ggacgttata attttaggtc tgcatagtgt agtgataatc ttagtgaata ctattgaagt
     1381 ggtcaacaaa aactggccac c
//
LOCUS       DAPHDX010000311         1397 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPHDX010000311 DAPHDX010000000
VERSION     DAPHDX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.316"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..902)
                     /locus_tag="RH058_004591"
     CDS             complement(<1..902)
                     /locus_tag="RH058_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3140022.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNAR"
     gene            complement(1213..1317)
                     /locus_tag="RH058_004592"
     CDS             complement(1213..1317)
                     /locus_tag="RH058_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDV3140023.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT          409 a          308 c          315 g          365 t
ORIGIN      
        1 cgggcgttat aaatcgtacc gtcgtaagtg cctagctgaa taacggcgag gcggaaagga
       61 cgagcctctt ttgcccgctg cggtgcaact tctgtaatca gctcacgcag gtaactttct
      121 tcaaaacagt gagcgtcaat accaccgata aaaccatacg ggttacgtgc ggtttccaga
      181 taaaccggtg tcgcgccagc ctgtagcaac gcaccgtggt ggttagattt atggttattg
      241 cggtcaaaca acaccagatc gcccggcgtc agcaatgcgt ttaagaccac tttattagaa
      301 gatgaagtac cgtttaaaac gaaataggtt ttatcggcat taaagacttt agccgcatgc
      361 tgttgtgcaa tacatggcgc accttcatga atcagcagat cgcccattgc tacgtcagca
      421 ttacacaagt cagagcggaa gagcatttcg ccaaagtatt ccacgaactg attgcctgcc
      481 ggatggcgac ggaaaaactc acccccctga tgaccagggc agtcaaaagc actattgccc
      541 tgattaacat aatcgaccag tgcgcggaag aagggcgggc gcagttgcgt ttcataatgg
      601 ctggcagcgg tttctaactg acgtccataa aactctttac gtgattcgca atgttcgaag
      661 acaccagaaa tacgtggcag atattctgcg ggtacacgtt cttcattttc cgttgcaata
      721 aaaacaggaa tgtcatagcc agtagcgtct atttcatcga gttttccacg ttcaacatca
      781 ctgactgata aaataatggc ggcaacgtca atataattac tttcgtctat attcacgcat
      841 gcacgatgag tggaaaaaca atccgggcaa gaacgactaa cggcaatttt taattctgac
      901 attttgttca tctctttatt tcaggtaata gcaggtcctc aatttctcaa aggagaaatc
      961 gatcacgccg gaaaaaaagc aaagctccac cgctgataaa taaatcagta gtttgctttt
     1021 tgcagatgca ataacccaga ccagcccggt aactggctat actcgtcata cctcaagttg
     1081 caagtgctgc gactcgaatt atttagagtt taaaagggtt agcagtatat gagcaccgag
     1141 gaatacaggt attaacctgt aatgcttaaa gtcagggaga cctgtacgtt ggggtgccct
     1201 ttagtcagaa gactagcggg cattaaagaa atgaaagtca aaactattac gatgggcaaa
     1261 cttcatgata tgggttgtcc gccttatatg aggcattaag cggttattat tttccattta
     1321 cctttacctc ttgctgtgtc agttgaaggt agagttattc tatccatgaa atggctataa
     1381 tctgtgaaga agatcaa
//
LOCUS       DAPHDX010000312         1374 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPHDX010000312 DAPHDX010000000
VERSION     DAPHDX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.317"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..361)
                     /locus_tag="RH058_004593"
     CDS             complement(<1..361)
                     /locus_tag="RH058_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDV3140024.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNT"
     gene            complement(582..1313)
                     /gene="bluR"
                     /locus_tag="RH058_004594"
     CDS             complement(582..1313)
                     /gene="bluR"
                     /locus_tag="RH058_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV3140025.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          353 a          317 c          308 g          396 t
ORIGIN      
        1 gcgtattatc aatggcgtag taactgcgtt tgttatgatt aacatcagta acccaccgga
       61 aaacgcccgc gcctgctagt gttgaacagt attcccgaaa tgtagatttt ccgcaaatat
      121 gaagcaatgc ggcctctttt attttagcag ggttcttggt cgtactaact tttaacaggt
      181 tcctggttcc tcttaataac aaaaccgtgt catcgtgagt aataattctg atgttatccg
      241 tagccagata ataaatgtaa tgtgcaatac ggtgatgttt taattctgaa taaaaccagg
      301 agaagttttg ctcttttctc acttgctcaa acatcttttg aaaaacaacg acctgatcca
      361 tcaggataat aacctcttgt tagttgtgag actgcgtagt gtgcacgatc ggttttacca
      421 cttcaatctg gtctgtcctt tggctgtgat atgtacagag tgtgatagag ggaatatctg
      481 aattctcccg gtgagcattt tgcaacggac cagctccggt acaaacgctg ttgtgggttc
      541 agattataac attctgtcta aggggcggga taaaggtgaa attagggggc atgaaagatg
      601 actttataac cttgctcacc ccaatgttgt aaaagttcgt tttgccttct cgttggtgcc
      661 atgcctgtcc agacaatcaa tgtttgcgtc gggaacagtt ccgggcgcgg cgattcaatg
      721 ggatcagcaa gaacagaaat gtgccatcct gacaatgata aacgccatgc ttccagccac
      781 aatcgtgctc tctcttcaac attccacgcc atcagaatgg cttctttacc gggcttacgg
      841 cgcatttcga aaagcgaggt tgctgcgtat tcaattaatg cgccgtcaaa catactgctc
      901 ataatgcggg aggtgttgtg atcaagcacg agacgctggc gaacaggaag gtaaacatga
      961 ttaatcaatt gatctactgg gtactctcga cccagtgaaa taattctcgc gcgtagtttg
     1021 gcaggattag ccatgcgaag aattgacatc atctcttctt gcaggcggct ccagtcatct
     1081 tccgtatcct ggctggtggt ttccagtaat gctttaactt tgcctacagg gacgccatta
     1141 cttatccaac gcttgatctc ttcgatgcgt tgtatgtctt cttcatcaaa gagtcggtgt
     1201 ccgccttcac tgcgctgtgg ttttaacaaa ccgtagcggc gttgccaggc ccggagagtg
     1261 acaggattaa tcccgcaacg ttcagcaaca tcaccaatgc tgtaataagc cacaattcct
     1321 ccttgcggtc acaaatctcc gtcgcctgta cacgacccaa taatactttg taca
//
LOCUS       DAPHDX010000313         1365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPHDX010000313 DAPHDX010000000
VERSION     DAPHDX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.318"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..1280
                     /gene="bluF"
                     /locus_tag="RH058_004595"
     CDS             69..1280
                     /gene="bluF"
                     /locus_tag="RH058_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV3140026.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          397 a          291 c          288 g          389 t
ORIGIN      
        1 aagaaagaca caagaagata tgtctgttac cgtcttactc tcgcctcacc cattaccctg
       61 gattgttaat gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca
      121 aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag
      181 ggatcttact gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg
      241 ttaaaatgat atatcgggct atatgccagg atccacggca ctataatatt gttgagctgc
      301 tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc
      361 gcctgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc
      421 agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac
      481 aatcaaccta tttcgagatc cctgccgaag actcctggct ttttatcgct gacggatctg
      541 ataaagaact tgattcctgt accctttcac caactataaa cgaccacttt gcctttcatc
      601 ctattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg
      661 aagatagccc atcagccata gcggttgggc agcgtaaaga cggggaaatc tacacagcgg
      721 atctcaaaag taaggcgctt gcattcgcga tggcacacgc acttgagctc ggtgataaaa
      781 tgatttcaat caatctatta cctatgaccc ttgttaacga acctgacgca gtctcttttt
      841 tacttgatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg
      901 aaagtgaagt tatatctcgg tttgatgagt ttgcagaagc gattaaatcg cttaaggctg
      961 ccggtatcag tgtagcaatt gatcattttg gcgcaggttt tgccggtttg ttactcctgt
     1021 cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa
     1081 gcgggccacg gcaggcaatt attcaagcga tcataaaatg ctgtacatca cttgaaattc
     1141 aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa
     1201 ttgagatgtt tcagggagat ctgtttgcta aagctaaatt gaatggtatc ccttccatag
     1261 cgtggccaga gaaaaaataa ttttcagcac attctttcac atgatttcag taaatcatcg
     1321 tgaaaatgaa tacgttacgt ttaaatttaa tccacattaa aaata
//
LOCUS       DAPHDX010000314         1364 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPHDX010000314 DAPHDX010000000
VERSION     DAPHDX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.319"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            61..1266
                     /gene="tet(B)"
                     /locus_tag="RH058_004596"
     CDS             61..1266
                     /gene="tet(B)"
                     /locus_tag="RH058_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV3140027.1"
                     /translation="MNSSTKIALVITLFDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          327 a          253 c          322 g          462 t
ORIGIN      
        1 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
       61 atgaatagtt cgacaaagat cgcattggta attacgttat tcgatgccat ggggattggc
      121 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
      181 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
      241 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
      301 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
      361 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
      421 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
      481 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
      541 ttttttatcg ctgcgttgct aaatattgtc actttccttg tggttatgtt ttggttccgt
      601 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
      661 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
      721 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
      781 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
      841 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
      901 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
      961 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     1021 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     1081 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     1141 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     1201 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     1261 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     1321 cccaagaggg cattttttac gataaagaag atttagcttc aaat
//
LOCUS       DAPHDX010000315         1356 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPHDX010000315 DAPHDX010000000
VERSION     DAPHDX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.320"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            245..>1356
                     /gene="pgaA"
                     /locus_tag="RH058_004597"
                     /gene_synonym="hmsH"
                     /gene_synonym="ycdS"
     CDS             245..>1356
                     /gene="pgaA"
                     /locus_tag="RH058_004597"
                     /gene_synonym="hmsH"
                     /gene_synonym="ycdS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="outer membrane protein essential for the synthesis
                     of poly-beta-1,6-N-acetyl-D-glucosamine (PGA); adhesin
                     required for biofilm formation; PgaA (HmsH) seems to
                     translocate and/or dock PGA to the cell surface; member of
                     the hmsHFRS (ycdSRQP or pgaABCD in Escherichia coli)
                     operon in Yersinia; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV3140028.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGA"
BASE COUNT          388 a          313 c          297 g          358 t
ORIGIN      
        1 attttgaaaa aggcattggg atttatgccg tattcctgaa gatcctcatc attggaatgg
       61 attttcgggc gagaaaagga ttttatatgg acactctgct catcatttct tcttctcatc
      121 atcaacaatt cacgtctctc ttccgcgttt aataacggat tatgaggtgc aaaaatatct
      181 ttcttttcag ttacctgtaa ttagatacag agagagattt tggcaataca tggagtaata
      241 caggatgtat tcaagtagca gaaaaaggtg cccgaaaacc aaatgggctt tgaaacttct
      301 tactgccgca tttttagcag cgagtcccgc ggcgaagagt gctgttaata acgcctatga
      361 tgcattgatt attgaagctc gcaagggtaa tactcagcca gctttgtcat ggtttgcact
      421 aaaatcagca ctcagcaata accaaattgc tgactggtta cagattgcct tatgggccgg
      481 gcaagataaa caggttatta ccgtttacaa ccgctaccgt catcagcaat taccagcgcg
      541 tggttatgca gctgtcgccg tcgcttatcg taacctgcaa caatggcaaa actcgcttac
      601 actgtggcaa aaggcgctct ctctggagcc gcaaaataag gattatcaac ggggacaaat
      661 tttaaccctg gcagatgctg gtcactatga tactgcgctg gttaaactta agcagcttaa
      721 ctctggagca ccggacaaag ccaatttact cgcagaagcc tatatctata aactggcggg
      781 gcgtcatcag gatgaattac gggcgatgac agagtcatta cctgaaaatg catctacgca
      841 acaatatccc acagaatacg tgcaggcatt acgtaataat caacttgctg ccgcgattga
      901 cgatgccaat ttaacgccag atattcgcgc tgatattcat gccgaactgg tcagactgtc
      961 gtttatgcct acgcgcagtg aaagtgaacg ttatgccatt gccgatcgcg ccctcgccca
     1021 atacgctgca ttagaaattc tgtggcacga taacccagac cgtactgccc agtaccagcg
     1081 tattcaggtt gatcatcttg gcgcgttatt aactcgcgat cgttataaag acgttatttc
     1141 tcactatcag cgattaaaaa agacggggca aattattccg ccctgggggc aatattgggt
     1201 tgcatcggct tatctcaaag atcatcagcc gaaaaaagca cagtcaataa tgaccgagct
     1261 cttttatcac aaggagacca ttgccccgga tttatccgat gaagaacttg cggatctctt
     1321 ttacagccac ctggagagtg aaaattatcc gggcgc
//
LOCUS       DAPHDX010000316         1328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPHDX010000316 DAPHDX010000000
VERSION     DAPHDX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.321"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            193..999
                     /locus_tag="RH058_004598"
     CDS             193..999
                     /locus_tag="RH058_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3140029.1"
                     /translation="MMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIAAPECIDIK
                     GAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVLPDEYIALE
                     FKGMSGAGAIEVDKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKINSRTIKNTS
                     VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIFHKDGTATE
                     FNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
     gene            complement(1042..1242)
                     /gene="glgS"
                     /locus_tag="RH058_004599"
     CDS             complement(1042..1242)
                     /gene="glgS"
                     /locus_tag="RH058_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV3140030.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          400 a          277 c          267 g          384 t
ORIGIN      
        1 gactgctatg ctgaacatga agggggaaat accgtagtca ttggttacgt accaagaatt
       61 tctatcccca gtaatggtaa aaaaggtgat aagatctggc aaagcagtga gtattttatg
      121 aatgtcttct gtaataatgc attacctgcc ccatcacctg gagaagaata cccatcagcc
      181 tggactaata taatgatgtt tttaccaggc ggtcaggact tttataatca gaactcctat
      241 atttttggcg taacatacaa tggagttgat tatgattcga ctgcccctaa tgctatcgca
      301 gcaccagaat gcattgatat aaaaggagcg ggtaccttta ataatcacta taaaaacccc
      361 gctgtctgta gtggaggtcc tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa
      421 ctctatatta aattagcaaa aaatgccaat agagttaata aaaacctggt gttacctgat
      481 gaatatatcg ctctagagtt taaaggtatg agtggcgcag gagctataga agtagacaaa
      541 aatttgacct tcaggattcg tggattaaat aatattcatg tccttgactg ctttgttaat
      601 gtcgcactgg aaccagctga tggcgttgtt gactttggta aaataaattc ccgaacaatt
      661 aaaaatacca gcgttagtga gacgtttagt gtagtcatga caaaagatcc tggtgcggcc
      721 tgtactgagc aattcaatat tttagggagc tttttcacta cggatatttt gagtgattat
      781 agccatctgg atatgggtaa tggactgcta ttgaagatat ttcataaaga tggaacagca
      841 acggaattta accgcttctc acaatttgct tctttttcat cgtctagtgc accttcggtc
      901 accgcaccat tcagggcaga acttagtgcg aatccggcag agactgttgt tgagggaccg
      961 tttagtaaag acgtcatcct caaaatcacc tataactagc atctaataca aacactaaaa
     1021 cgggccatca ggcccgttat atcagtgctc taactccagc tctcttgcct gcatcatctg
     1081 ttgacaatac caggcataac gtgcgcaaaa ccaggttcta tgttcttcat ccatgttacc
     1141 agtaacggca agaatatcga ccgggcgacc ttctgcgtgc attctggcaa aactacgcgc
     1201 caggaaatcg aaattattta aagaattaag actatgatcc ataagcacat cctccggtta
     1261 tcgcatacct tttaaggcca tatgcttttt ctgatttcag tattccccaa tcagtgtagt
     1321 gcctttaa
//
LOCUS       DAPHDX010000317         1292 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPHDX010000317 DAPHDX010000000
VERSION     DAPHDX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.322"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            671..862
                     /locus_tag="RH058_004600"
                     /pseudo
     CDS             671..862
                     /locus_tag="RH058_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(957..1145)
                     /locus_tag="RH058_004601"
     CDS             complement(957..1145)
                     /locus_tag="RH058_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HDV3140031.1"
                     /translation="MKDNILTFLTELKTPCRTTEIAIHFGLSAYQARYYLMTLEKEGK
                     IKRSPVRRGASTLWEMNS"
BASE COUNT          326 a          286 c          297 g          383 t
ORIGIN      
        1 gagtacaaac tgctaaacaa aacacgatcc actttcattg taatatacat ccggagctat
       61 gttggacttt gtatcgagtt tgaaaaaatt ccaagttatg gaagtctacc attttgggca
      121 gaaaaaccat ttactatgac taaatataat gatcatcgtc acaaaaaaga atttgagtgt
      181 gtagtcttgt cttttaggag cagtccagtc attggcgacc acatgttgtt gaacggcatc
      241 ggtacttact ggtgctaccg aaacgctgtc tggaataact cctggctctt ttgcctgagg
      301 gccctgaacc cgcctttgag cttgttcatc atgcaattat tggttcaccc tatattgtga
      361 acgccgattg ggtttgaaca ggcctgctga tcatcggact aattgcacta tccccgattc
      421 tgactgcttc ggggattaac agcggcttgt tgcctttagg aacaattttt tagatcctgt
      481 taatcaaacg tactggtgag cggctgggcc aatccgtgat cgaatagagc ggaatacgca
      541 gtgtagtgac ttttaacgtc aggtgatgca taagggcctt tcgctgagag accctgttta
      601 cacaattaaa taagtcaagc ccgaccagca attttgacgt tgacggcagg attattgagg
      661 ggcaatcaat gtgttattcg gcagcgatta cggtagtgaa cggcgctcta ctgtacggac
      721 tgagcgggac ctgctgtctg aatggtatct gcgccatatc ctgagcgtac tgccggaatg
      781 gccttccagc cgtgttgatg aactcctgct gcatattagc atggaggtgt tcggtgagtg
      841 ccggacgacg tattctctgt aataattctt acacctgcga aatggcatgg ttcatcgtct
      901 gacaacccgg gaaaaccgtt gaccattttt atatttcagg cacagatatt ttcccgtcac
      961 gaattcattt cccataaggt actggcacct ctccttactg gcgagcgttt tatctttccc
     1021 tccttttcga gagtcatcag gtaatatctg gcctgatagg cactcagccc aaaatgtatg
     1081 gcaatttctg ttgtccggca cggagttttc agctccgtga ggaacgtcag tatattgtct
     1141 ttcatattct gttaattccc gtgtggacct gaatatcgtg catggtatat caaaccatac
     1201 gaactcttgt cgttgttaat ttcagccatg taaagccatc agggctggca actgacgtat
     1261 ataatacaac gattattttc tgccgaaaga ag
//
LOCUS       DAPHDX010000318         1280 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPHDX010000318 DAPHDX010000000
VERSION     DAPHDX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.323"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            134..736
                     /locus_tag="RH058_004602"
     CDS             134..736
                     /locus_tag="RH058_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV3140032.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(775..1200)
                     /gene="nudI"
                     /locus_tag="RH058_004603"
     CDS             complement(775..1200)
                     /gene="nudI"
                     /locus_tag="RH058_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV3140033.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          354 a          284 c          270 g          372 t
ORIGIN      
        1 tttggcaaaa ttttgaggca ttttgccgac atcgtaggat ttttaatatt acaccaactg
       61 ctaattatcg ccagaaatgt cgctcaattt tacggtaatt gtctggttgc gcttgtctat
      121 aggtggagtt tacgtgttag ctttttgccg ctcatcattg aagtcaaaaa aatatttcat
      181 cattttactg gcgctcgctg caattgccgg actgggtact catgccgcct ggagtagcaa
      241 tggtttgcca cgtatcgaca ataaaacatt ggccagactg gcacagcagc acccggttgt
      301 cgttttgttt cgccatgctg aacgttgcga tcgttcaacc aatcaatgct tgtcagataa
      361 aacaggtatt acggttaaag gtacccagga tgcccgtgaa ctgggcaacg cttttagcgc
      421 tgacatccct gatttcgatc tttattccag taataccgtc cggaccattc agtcggcgac
      481 ctggttttcc gcgggtaaaa agctgacggt agataaacga cttcttcagt gcggtaatga
      541 gatttatagt gcaattaagg acttacaaag caaagcgcct gataaaaata tcgttatttt
      601 cacccataat cattgcctga catatattgc taaaaataag cgtgatgcga catttaaacc
      661 tgattatctg gatggtttag tcatgcatgt ggaaaaaggc aaagtttatc tggatgggga
      721 attcgttaac cactaaattt tcaatctgac agccagtgat ggctgtcatc attgttaaag
      781 aagacctttc aaacgtaacg tttttcgggt ggcaacattt aaatcataat gcaccagatc
      841 ttcaggtttt acccacgcgt agtcctgaaa ctcttcgttt attttcactt ctcggttggc
      901 agaaacgcag tcaaaaatca ggtaaatcat ataaatctct tccttgcgac catctgcata
      961 cgtcttggtg cgaatatcat cgctgaaggt ccacggcgtg atttctgtca aaagcagctg
     1021 ttctcccagt tcttcgcgaa tttcgcggcg tagtgcctct tcaattcgtt cgccagactc
     1081 cacgccgcca cccgaaagcg cccattgacc ggggaaaacg ccgcgatcgt cggccatttt
     1141 acacagcaaa taagcaccat cattttgaat caaagggcat acaatagtcc gttgtcgcat
     1201 aggggtattc cttataaata aaaagaaacc atgagaatac ggtaactcag aaatatgtga
     1261 aagtgacagt aatctctttt
//
LOCUS       DAPHDX010000319         1245 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPHDX010000319 DAPHDX010000000
VERSION     DAPHDX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.324"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            24..1232
                     /locus_tag="RH058_004604"
     CDS             24..1232
                     /locus_tag="RH058_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDV3140034.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGFSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFAAAWDCRYPQISRSWQANWPNLATFFAYPTDIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFII
                     EFGDRLDGHF"
BASE COUNT          338 a          316 c          326 g          265 t
ORIGIN      
        1 cagcatcaaa tgaaggttaa tttatggacg aaaaacagtt acaggctctg gctaacgaac
       61 tggccaaaaa cctcaaaacc cctgaagacc tcagtcagtt tgatcggctg ctgaaaaagc
      121 tcagcgttga agccgctctc aatgcagaga tgacacacca tcttgggtat gagaaaaatc
      181 agtccagacc aggagctaac tcccgcaacg gtttttccac aaagaccgtt atcacaggcg
      241 acggtccact ggaactgcgt actccgcgcg atcgtgacgg taccttcgaa ccacaactgg
      301 taaagaaaaa tcagacccgt attaccggga tggataacca gatcctctcg ttgtatgcca
      361 aagggatgac cacccgtgag atagctgctg cgttcaaaga actgtatgac gcagatgttt
      421 caccggcact gatatcaaag gttaccgatg ccgtgatgga gcaggttgta gaatggcaaa
      481 accgaccact ggatgctgtt taccccattg tttatcttga ctgtatcgtc ctgaaagttc
      541 ggcaggacag tcgcgtcatc aacaaatcgg tgttcctggc actgggcatc aatatcgaag
      601 gtcagaaaga actgctgggt atgtggctgg ccgaaaatga aggggcgaag ttctggctca
      661 atgtgctgac tgaactgaaa aaccgcggtc tgaacgatat cctcatcgcc tgtgtggatg
      721 gcctgaaagg cttcccggat gccatcaaca cagtatatcc gaaggcccgc atccagttat
      781 gcatcgtgca tatggtgcgc aacagcctgc gcttcgtgtc atggaaggac tacaaagccg
      841 tcactcgcga cctgaaagcg atttatcagg ctcccacgga agaggcaggc cagcaggcac
      901 tggaagcgtt cgctgcggcc tgggactgtc gctatcctca gataagccga agctggcagg
      961 ctaactggcc gaatcttgcc acgttcttcg cttatccaac ggacatccgc aaagtgatct
     1021 atacgacgaa tgccatcgag tcgctaaaca gcgtgatccg ccatgcgatc aaaaagcgta
     1081 aagtgttccc gacagacgac tcggtgaaaa aagtggtgtg gctggcaatc caggcggctt
     1141 cacagaaatg gacgatgcca ctgcgggact ggcgtatggc aatgagccgc tttattatcg
     1201 agttcggtga ccgcctggac ggccacttct gagaaaaggt attta
//
LOCUS       DAPHDX010000320         1230 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPHDX010000320 DAPHDX010000000
VERSION     DAPHDX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.325"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..776
                     /locus_tag="RH058_004605"
     CDS             <1..776
                     /locus_tag="RH058_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3140035.1"
                     /translation="EHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATI
                     LANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLP
                     SIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAY
                     LRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPG
                     FAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            773..>1230
                     /locus_tag="RH058_004606"
     CDS             773..>1230
                     /locus_tag="RH058_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3140036.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMYSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELFGASLSPVQIGLATIGVLWICTVANFGGARITGQISSIT"
BASE COUNT          282 a          300 c          340 g          308 t
ORIGIN      
        1 gggaacactg gcattctttt gaaggctatg cagagaatca atactttgtc gatccttgca
       61 aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa gcgttcggcg
      121 tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg
      181 atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaaa ctgcagcaac
      241 ttgttgccct gctggtacgc ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag
      301 tgctgccttc catctacaaa cagcatgaag agcgctacac cggttatacc ctgcgtcagt
      361 tgtgtcagga aatgcacgat ttgtatgccc gccacaacgt gaaacaactg caaaaagaga
      421 tgttccgtaa ggaacacttc ccacgcgtca gcatgaatcc acaggaagcc aactacgcct
      481 atttacgcgg tgaagtggag ctggttcgtc tgccggatgc cgaaggccgt atcgctgccg
      541 agggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt tccgggtgag atctggggtg
      601 gtgctgttct gcgttacttc agcgctctgg aagaagggat caacctgctg ccaggttttg
      661 caccggagct gcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa gtctggtgct
      721 atgtcatcaa gccccgtgat gcgcaaagca ccctgttaaa aggggaaaaa ttatgagtca
      781 ggctaaatcg aacaagatgg gcgttgttca gttaaccata ctgacgatgg tcaacatgat
      841 gggctctggc atcatcatgc tgccgactaa actggcagag gtcgggacga tctcgattat
      901 ctcctggctg gttacagctg taggctcaat ggcgctggcc tgggccttcg caaaatgcgg
      961 tatgtatagc cgtaaatcag gtggtatggg cggttatgcc gaatatgcgt tcggtaaatc
     1021 cggtaacttt atggcgaact acacctacgg tgtctcgctg ctgattgcca atgtcgccat
     1081 tgcgatttcc gccgttggtt acggcaccga actgttcggc gcgagtttgt cgccagtgca
     1141 gattggtctt gcgaccatcg gagtgctgtg gatttgtact gtggctaact ttggtggtgc
     1201 gcgcattacc gggcaaatca gtagcattac
//
LOCUS       DAPHDX010000321         1230 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPHDX010000321 DAPHDX010000000
VERSION     DAPHDX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.326"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..776
                     /locus_tag="RH058_004607"
     CDS             <1..776
                     /locus_tag="RH058_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3140037.1"
                     /translation="EHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATI
                     LANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLP
                     SIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAY
                     LRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPG
                     FAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            773..>1230
                     /locus_tag="RH058_004608"
     CDS             773..>1230
                     /locus_tag="RH058_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3140038.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSIT"
BASE COUNT          287 a          291 c          337 g          315 t
ORIGIN      
        1 gggaacactg gcattctttt gaaggctatg cagagaatca atactttgtc gatccttgca
       61 aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa gcgttcggtg
      121 tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg
      181 atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaag cttcagcaac
      241 ttgttgccct gctggtacgc ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag
      301 tgctgccttc catctacaaa cagcatgaag agcgctacgc cggttatacc ctgcgtcagt
      361 tgtgtcaaga aatgcatgat ttgtatgccc gtcacaacgt gaaacaactg caaaaagaga
      421 tgttccgtaa ggaacacttc ccacgcgtaa gcatgaatcc gcaagaagcc aactacgcct
      481 atttacgcgg tgaagtggag ctggttcgtc tgccggatgc agaaggccgt atcgctgccg
      541 aaggtgcgct tccttatcct ccgggtgtgc tgtgcgttgt tccgggtgaa atctggggtg
      601 gtgctgttct gcgttacttc agcgctctgg aagaagggat caacctgttg ccaggttttg
      661 caccggagct tcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa gtttggtgct
      721 atgtcatcaa gcctcgtgat gcgcaaagta ccctgttgaa aggggaaaaa ttatgagtca
      781 ggctaaatcg aacaaaatgg gcgtcgttca gttaaccata ctgacgatgg tcaacatgat
      841 gggctccggt atcatcatgc tgccgacaaa gcttgccgaa gttgggacaa tttcaattat
      901 ctcctggctg gtgacagccg taggctcaat ggcactggca tgggcattcg caaaatgcgg
      961 tatgttcagc cgtaaatcag gtggtatggg cggttatgcc gagtatgctt tcggtaagtc
     1021 cggcaatttt atggcgaact acacctacgg tgtctcgctg ctgattgcta acgtcgcgat
     1081 tgctatttcg gcggttggtt acggcaccga actgctcggc gcgagtttgt cgccagtgca
     1141 gattggtctt gcgaccatcg gagtgctgtg gatttgtacc gtggctaact ttggtggtgc
     1201 gcgcattacc gggcaaatca gtagcattac
//
LOCUS       DAPHDX010000322         1229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPHDX010000322 DAPHDX010000000
VERSION     DAPHDX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.327"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..706
                     /locus_tag="RH058_004609"
     CDS             32..706
                     /locus_tag="RH058_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3140039.1"
                     /translation="MLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYIT
                     QDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDI
                     AKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMD
                     TKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRT
                     SRGIRK"
     gene            703..>1229
                     /locus_tag="RH058_004610"
     CDS             703..>1229
                     /locus_tag="RH058_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140040.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYEC"
BASE COUNT          395 a          242 c          282 g          310 t
ORIGIN      
        1 atggtgcgca gaatacgatg aatggggcaa cctgctgaat gaagagaacc cgcatcagct
       61 gcagcagctt atccgcctgc cggggcagca gtatgatgag gagtccggcc tgtattacaa
      121 ccgccaccgc tattatgacc cgctgcaggg gaggtatatc actcaggatc cgattgggct
      181 gaagggggga tggaattttt atcagtatcc gctgaatccg gttcagtata tagattcaat
      241 gggactggca tcaaaatatg gacacttaaa taatggcgga tatggagcga gacccaacaa
      301 accgcctacg cccgatccaa gtaaattgcc ggacatagcg aaacaattaa gactgccata
      361 tcctattgac caggccagta gtgcgcctaa tgttttcaaa acattcttca gagcattaag
      421 cccttacgac tacacactgt attgcaggaa gtgggtaaaa ccaaatctga cttgtacgcc
      481 acaggatgat tcccagtatc cagggatgga tacaaagaca gcaagtgatt acctgccaca
      541 gacaaattgg ccaacaactc aattaccacc aggatatact tgtgcagaac cctatttatt
      601 cccagacatt aataaacccg atgggccagc aacagcaggg atagatgatt tgggtgaaat
      661 tttagctaag atgaaacaga gaacatcgag aggaataaga aaatgaaaag agttttgttc
      721 tttttgctga tgatatttgt tagttttggt gttatagctg attgcgaaat acaagctaaa
      781 gatcatgatt gttttactat tttcgctaag ggtactattt tttccgcgtt tcctgtctta
      841 aataataaag ccatgtggag atggtatcag aatgaggaca tcggtgagta ttattggcaa
      901 acagaactgg gtacatgtaa aaataataaa ttcacaccaa gcggggcgag actcttaatt
      961 cgcgtcgggt cactacgcct taatgaaaat catgccatca aaggaacgtt gcaggagttg
     1021 ataaatacag cagaaaaaac ggcgtttctc ggcgatcggt ttagaagtta tataagagcg
     1081 ggtatctatc aaaaaaagag cagtgatcca gttcagttgt tggcagtcct tgataattca
     1141 atcatggtaa aatattttaa agatgagaaa ccaacttatg ctcgaatgac agcacatctc
     1201 ccgaacaaaa atgagtctta cgaatgttt
//
LOCUS       DAPHDX010000323         1229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPHDX010000323 DAPHDX010000000
VERSION     DAPHDX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.328"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(95..>1229)
                     /locus_tag="RH058_004611"
     CDS             complement(95..>1229)
                     /locus_tag="RH058_004611"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HDV3140041.1"
                     /translation="KIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPG
                     YSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVV
                     HNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADI
                     EIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAA
                     HKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADL
                     VVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSD
                     ILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          382 a          244 c          207 g          396 t
ORIGIN      
        1 ttgaaccaaa tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa
       61 aaataaaaaa ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct
      121 cttcgaatct ttgagttacg ccatcccaac tgtagtgttc aaacacaaag tcctgacctt
      181 gctttgcaac tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg
      241 agtctgcagt cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg
      301 tcatcccccc acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca
      361 ccatgcaaaa tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat
      421 aacaatgcat cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt
      481 ttgcaatttc cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat
      541 aatcaccaat cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt
      601 gcatcaacag caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct
      661 cttcagggga gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt
      721 ctaaatcaat tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat
      781 actttttcaa gtataaacta ggcactatca ttttaacatt ttgctcaagc aattttggtt
      841 caaatgcatt atgcatatgt ataaccactc ttgcctgagg agctcgctta cgaatttgag
      901 tgtatagctt catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat
      961 gtgcaatatt caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa
     1021 ataaccgctt atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa
     1081 aggtgtaatt tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac
     1141 gctgagcaac ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa
     1201 aaataggagt aaccgtaaat attatttta
//
LOCUS       DAPHDX010000324         1218 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPHDX010000324 DAPHDX010000000
VERSION     DAPHDX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.329"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            144..1218
                     /locus_tag="RH058_004612"
                     /pseudo
     CDS             144..1218
                     /locus_tag="RH058_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          438 a          170 c          293 g          317 t
ORIGIN      
        1 cagaaagaca tgagagcaag ggacggggca gggcaagatc atgaaaattg tgatgtaaat
       61 cacgattttc atcttggctt taacgcctac aggtgatcaa aatttaacct ttggaataac
      121 taaaaagata aaaaaggacc caggtgagta ttcaaagttt gcttgattat atttcagtga
      181 tccctgatat acgacaacaa ggaaaggtta aacataaatt atctgctatt ttgtttctca
      241 ccgtatgtgc agtaattgca ggtgccgatg agtggcagga aattgaagat tttggacatg
      301 aaagacttga atggctaaag aaatatggtg attttgataa tggcattccg gtcgatgaca
      361 ccattgcacg cgttgtgagt aacattgaca gtttggcctt tgaaaagatg tttattgaat
      421 ggatgcagga gtgccatgaa atcactgatg gtgaaattat agcaatagat ggaaagacca
      481 taagaggctc ctttgataag ggaaaaagaa aaggagcaat ccatatggtg agtgcattct
      541 cgaacgaaaa tggtgttgta ctggggcagg tgaaaacgga agccaaaagt aatgagatta
      601 cagccattcc agagttgctt aacctactgt atttaaagaa aaatttgata accattgatg
      661 ctatgggctg tcagaaagat atcgcttcga agatcaaaga taaaaaagca gattatcttc
      721 tggcagtaaa aggcaatcag gggaaattac atcatgcatt cgaggaaaaa tttcctgtaa
      781 atgtgttttc taattataaa ggcgattcgt ttagtacgca ggagataagt catggaagaa
      841 aagaaacacg tttgcatatt gtcagtaacg taacgcctga acttttgtga ttttgaattc
      901 gaatggaagg gattaaaaaa gctttgtgta gcattgtcat tcaggcagaa gaaagaagat
      961 aaatcagcag aaggtgtaag catccgatat tatatttcat caaaggatat ggatgctaaa
     1021 gaatttgcac atgctatcag agcgcactgg ctgatcgagc acagtcttca ttgggtgtta
     1081 gatgtaaaaa tgaatgaaga tgccagccgg ataagaagag gaaacgcagc ctaaataata
     1141 tctggaataa agaagatggc actgaattta ttaagagatt gcaaagacat taagggtgaa
     1201 gaggaaaaga aagaaggc
//
LOCUS       DAPHDX010000325         1205 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPHDX010000325 DAPHDX010000000
VERSION     DAPHDX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.330"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..1105
                     /gene="ompC"
                     /locus_tag="RH058_004613"
     CDS             8..1105
                     /gene="ompC"
                     /locus_tag="RH058_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3140042.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          337 a          269 c          268 g          331 t
ORIGIN      
        1 gctcacgatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag tactgatggc
       61 gatgtctgct caggcagctg aaatttataa taaagacagt aacaagctgg atctgtacgg
      121 gaaagttaat gctaagcact acttctcctc taatgatgca gatgatggtg atactactta
      181 tgcccgtctt ggcttcaaag gtgaaaccca aatcaacgat caactgactg gtttcggtca
      241 gtgggaatat gaattcaaag gcaaccgcgc tgaatctcaa ggttcctcca aagacaaaac
      301 ccgtcttgca tttgcaggcc tgaaattcgg tgactacggc tcaatcgatt acggccgtaa
      361 ctacggtgta gcatacgaca tcggtgcgtg gactgacgtt ctgccagaat tcggtggcga
      421 tacctggacc caaacagatg tgttcatgac tggtcgcacc actggtgttg caacctatcg
      481 taacaacgac ttctttggtc tggttgatgg tctgaacttt gctgctcagt accaaggcaa
      541 aaacgatcgt agcgatttcg ataactacac tgaaggtaac ggtgatggct tcggtttctc
      601 tgctacctat gaatacgaag gattcggtat cggtgcaact tatgcgaaat ctgatcgtac
      661 cgacactcaa gttaatgcag ggaaagttct tcctgaagta tttgcttccg gtaaaaatgc
      721 agaagtttgg gccgcaggtc tgaaatatga cgctaacaac atttacctgg ccactaccta
      781 ttctgaaacc cagaatatga ctgtatttgc tgatcacttc gttgctaata aagcccaaaa
      841 cttcgaagct gttgcacaat atcagttcga tttcggtctg cgtccgtccg ttgcttacct
      901 gcaatctaaa ggtaaggatc ttggagtatg gggcgatcag gacttagtca aatatgttga
      961 tgtaggtgca acctattact tcaacaaaaa tatgtctact ttcgttgatt acaaaatcaa
     1021 cctgcttgac aaaaatgact tcactaaagc actcggtgta agcactgatg acatcgttgc
     1081 tgtaggtctg gtttaccagt tctaatctga ttacgaaaaa gatatgttgc gggaggcgtt
     1141 gcctccccaa catataagtg gctccctcaa gccacttcct ttagaagcac aaccttgctt
     1201 ctaac
//
LOCUS       DAPHDX010000326         1194 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPHDX010000326 DAPHDX010000000
VERSION     DAPHDX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.331"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..240)
                     /locus_tag="RH058_004614"
     CDS             complement(<1..240)
                     /locus_tag="RH058_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3140043.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSVALGM"
     gene            297..965
                     /locus_tag="RH058_004615"
     CDS             297..965
                     /locus_tag="RH058_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV3140044.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          350 a          272 c          221 g          351 t
ORIGIN      
        1 catacccaga gctactgatg tatcaactcg attcaacaat acccgatatt tattccatgc
       61 ctccagcaac gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg
      121 cgcaatatac tcactgaatt cctggatgta gaactgtgta gtgacggtct tccagccatt
      181 cggctcctgc tgtatcgaag cataccaggc tatttcaata tcgctatgct gcggcagcat
      241 ttaacccctt gtaattcatc gccataattg atttaattca caaataaaac tataacatgg
      301 tgaaattaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa
      361 agctttatga ctggtgggtt ttgggagtat caaatcggct tgcatgggga tgtcctacaa
      421 aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg gatattggtg
      481 ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt
      541 tgaacgaagc tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata
      601 aaattaacca tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca
      661 tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa
      721 tacgcaatgc ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg
      781 gcgatggagt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag
      841 gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt
      901 tcgagaatgt taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa
      961 aatagcatcc aaccgcagca cgttcttgca taaggcgtgt ctgcggtttt tcaactattc
     1021 agatacatca ctcccatcac attcattcct ccgcatcata ggcatatagg ctatatcacc
     1081 ttgatatttt tcttcttcag ataaaaactg ttatctatgt atacttttaa acccaatccg
     1141 tgtagagtct ctgcataaga tagtttgcag ttgccacttc agcttgcgcc ataa
//
LOCUS       DAPHDX010000327         1185 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPHDX010000327 DAPHDX010000000
VERSION     DAPHDX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.332"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            118..225
                     /locus_tag="RH058_004616"
                     /pseudo
     CDS             118..225
                     /locus_tag="RH058_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(278..1036)
                     /locus_tag="RH058_004617"
     CDS             complement(278..1036)
                     /locus_tag="RH058_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV3140045.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          334 a          282 c          276 g          293 t
ORIGIN      
        1 gaaacagtga tcaggtgtac aacgccaccc gacggctgta ataacaaaaa atcccttgat
       61 gcctgtccct tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg
      121 ccgactaaac gctttgataa aaaacactga aagatggtgg tggtgctact ggcaatctgt
      181 ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa
      241 gacagccaga ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata
      301 aggttgtccg acgatcggta tgttccggtg cagactctgg aggttcagag ttccatgtga
      361 cgttgtaagc cactttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg
      421 agttcagcgt tgtgtcttcc gcgccaaaga cggaaacacc ctgcgtaaat ttagcgttgt
      481 cagtcaactg ccatgcataa gcaccggagg catagcccag cggctgagtt tcactggcat
      541 tgtcggtata tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg
      601 ggccattcaa aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt
      661 tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa
      721 aacggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat
      781 tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt
      841 cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat
      901 taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat
      961 cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg
     1021 tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc
     1081 ggaaactttc ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg
     1141 cgcgaataac ttagcaatgt attcttattt cattttttga ataag
//
LOCUS       DAPHDX010000328         1153 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPHDX010000328 DAPHDX010000000
VERSION     DAPHDX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.333"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..1028
                     /locus_tag="RH058_004618"
     CDS             48..1028
                     /locus_tag="RH058_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HDV3140046.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSH"
BASE COUNT          319 a          317 c          299 g          218 t
ORIGIN      
        1 gatatgagat catcatattc atccggagcg catcccagag ggacatcatg agccatcaac
       61 tcaccttcgc cgatagtgaa ttcagcacta agcgccgtca gacccgaaaa gagattttcc
      121 tctcccgcat ggagcagatt ctgccatggc agaatatgac cgctgtcatc gagccgtttt
      181 atcccaaggc gggcaatggc cgacggccct atccgctgga gaccatgctg cgtattcact
      241 gcatgcagca ttggtacaac ctgagcgacg gtgccatgga agatgccctg tacgaaatcg
      301 cctccatgcg cctgtttgcc cgattatccc tggatagcgc cctgccggat cgcaccacca
      361 tcatgaattt ccgccacctg ctcgagcagc atcaactggc ccgtcaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgaccca aggcactttg gtggatgcca
      481 ccatcattga ggcacccagc tctaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctagtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgcttcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gagcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgtc
      781 ccggcaaggt aaaaaccttg aagcagcatc cgcgcaagaa caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgtgccaagg tagagcaccc gtttcgcatc atcaagcggc
      901 agttcggctt cgtgaaagcc agatacaagg ggctgctgaa aaacgataac caactggcga
      961 tgttattcac cctggccaac ctgtttcggg tggaccaaat gatacgtcag tgggagagat
     1021 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     1081 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     1141 ccgagaagaa ttt
//
LOCUS       DAPHDX010000329         1111 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPHDX010000329 DAPHDX010000000
VERSION     DAPHDX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.334"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..282)
                     /locus_tag="RH058_004619"
     CDS             complement(<1..282)
                     /locus_tag="RH058_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140047.1"
                     /translation="MADKAILWALISASNKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAA"
     gene            complement(989..>1111)
                     /locus_tag="RH058_004620"
     CDS             complement(989..>1111)
                     /locus_tag="RH058_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3140048.1"
                     /translation="NRAIYWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          322 a          233 c          191 g          365 t
ORIGIN      
        1 agcggcaagt tggagtggat ttagattttt cagatatggt cttatgatct ttcccttact
       61 taataaatag catgtatagg attcggaaag tccagcatct attttgtacc tcattatatt
      121 agataaggat gtgagaaatt ccttgttatc attcgcagcc atatatgcca gtccgagttc
      181 agcctctgcg gccttacagg caaaatagct tagtgaacac gcttttcgtc cctctttatt
      241 actggctgat attagtgccc ataatattgc tttgtctgcc aaatctaact ctctctgaaa
      301 ttgtcttgca gtaactgcac tcgcagaaat agagaaaaaa atacaattgt taataacctt
      361 gccatatttt ttagtccgta actgttaatg tgctatctgg ctctgaaaat attaactcat
      421 ttaatttttg catgtcatta gataaaccag ttatacaacc ctcggaacag aatcgaggtc
      481 ctaaagacgg accaaaagcg tttaaacaac tatgtattga aaatccattg cgattataag
      541 ttcctgtatt aactgacggt actaatcgta tgccgtcagg ttttctattg cttgctcccg
      601 ggtcattaac attccaactc cataggcctt gaggtattgg ccatctattt ataaatgtgt
      661 acaacgggga ttatttttac attgcatatt attaccagaa gcgactggaa tactaagcac
      721 atcataaaaa ttatcatcac atggtatatc tgaaacacac gaaagcatac ctaaagttat
      781 actgtataaa caggtggcta atcccaaggg gatcgattcc agaaatcggg ttcaacggat
      841 actgataaaa attccaccta ccttccagcc caatcggagc ctgagtgtta taccgcccct
      901 gctgcggtta atgttaccga taatagttat agtacagccc tgattcatca tcatactact
      961 gctgcggcag tctgatgaac tattgctttt acggattctc cttattcttc cttctatcca
     1021 ttcgctttag ctttttgagc tcttgaagtt tgctctttgt atcaatatta ccgttattta
     1081 cagcgaattc catccaataa atagctctat t
//
LOCUS       DAPHDX010000330         1069 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPHDX010000330 DAPHDX010000000
VERSION     DAPHDX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.335"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1069
                     /gene="rhsB"
                     /locus_tag="RH058_004621"
     CDS             <1..>1069
                     /gene="rhsB"
                     /locus_tag="RH058_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV3140049.1"
                     /translation="ETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEG
                     LVTHWHYDEADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVAVHYRYDEKGRL
                     TGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLG
                     DTPLVDFTRDRLHRETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELI
                     RISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMW
                     PDNRIARDAHYLYRYDRYGRLT"
BASE COUNT          248 a          349 c          316 g          156 t
ORIGIN      
        1 atgaaacgcg gtatgaatac aacatcgccg gtgacctgac cgccgtcatt gccccggacg
       61 gcagcagaaa cgggacacag tacgatgcgt ggggaaaggc cgtccgtacc acgcagggcg
      121 ggctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg accagtgaaa
      181 acggcagcca caccaccttc cgttacgatg tacttgaccg gctgatacag gaaaccggct
      241 ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc cgcagcgagg
      301 atgagggtct ggtcacccac tggcactatg acgaagcaga ccgcctcacg caccgcaccg
      361 tgaagggtga aaccgcagag cggtggcggt atgacgaacg tggctggctg acagacatca
      421 gccatatcag cgaagggcac cgggtagcgg tgcattacag gtatgatgag aaaggccgcc
      481 tgaccggtga gcgccagacg gtgcatcacc cgcagacgga agcactgctc tggcagcatg
      541 agaccagaca tgcgtacaac gcgcagggac tggcgaaccg ctgtataccg gacagcctgc
      601 ccgccgtgga atggctgacc tacggcagcg gctggctggc aggcatgaag ctcggcgaca
      661 caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc agcttcggtc
      721 gttatgaact caccaccgct tacacccctg ccgggcagtt acagcgtcag cacctgaaca
      781 gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc cgcatcagca
      841 gcccgcgcca gacccggagt tacagctaca gcaccaccgg caggctgacc ggcgttcaca
      901 ccaccgcagc gaatctggat atccgcatcc cgtatgccac ggacccggcg ggcaaccgcc
      961 tgcccgaccc ggagctgcac ccggacagca ccctcagcat gtggccggat aaccgtatcg
     1021 cccgtgacgc gcactatctt taccggtatg accgttacgg caggctgac
//
LOCUS       DAPHDX010000331         1058 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPHDX010000331 DAPHDX010000000
VERSION     DAPHDX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.336"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1058
                     /gene="gltS"
                     /locus_tag="RH058_004622"
     CDS             <1..>1058
                     /gene="gltS"
                     /locus_tag="RH058_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV3140050.1"
                     /translation="FVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDAS
                     LQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNESP
                     FIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGGPVAR
                     HLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALF
                     KDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKF
                     GQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISAGHCGFGMGATPTAI
                     ANMQTVTKAFGPSHKAFLV"
BASE COUNT          280 a          200 c          227 g          351 t
ORIGIN      
        1 tttgtcgtaa aatttacccc gttcctacaa aaaaaccaca taccagaagc cgtcgttggt
       61 ggctttattg ttgcaattgt tctgttaatt attgataaaa catcaggtta ttcgtttact
      121 tttgatgctt cattgcaaag tttattaatg ctcacattct tttcctctat cgggctaagt
      181 tctgactttt ctcgactgat taaaggagga aagccgttag ttctattaac tattgcagta
      241 acgatcctaa tcgccattca aaatactgtc ggcatgagta tggctgtcat gatgaatgaa
      301 agtccattta ttggcttaat tgcaggttca attactctaa caggtggtca tggtaatgcc
      361 ggagcatggg gccctattct cgctgataaa tatggtgtaa caggcgccgt tgaattagcg
      421 atggcttgtg caacacttgg attagtgttg ggtggtttag ttggcggccc tgttgcccgt
      481 catcttttga aaaaggtctc tattcctaaa acaaccgagc aagagcgcga cactatcgtt
      541 gaagcttttg agcaaccaag cgtcaaaaga aaaatcaatg caaataacgt tattgaaacc
      601 atttcaatgc tgattatctg tattgttgtt ggtggctata tcagtgcatt gtttaaagat
      661 acttttctgc aactgcctac ttttgtctgg tgtttatttg tcggtattat tatccgtaat
      721 acactgactc atgtatttaa acacgaagtg tttgagccga ccgtcgatgt attaggtagc
      781 gttgctttat cgcttttctt ggcaatggcg ttaatgtcat taaaatttgg tcaattggca
      841 agcatggcag ggccagtatt aattatcatt gctgtacaaa ctgttgtcat ggtgctattt
      901 gcctgctttg tcaccttcaa aatgatgggc aaagattatg atgctgtcgt gatcagcgcg
      961 ggtcactgtg gctttggtat gggagcaaca ccaacagcaa ttgcgaatat gcaaacagtc
     1021 acaaaagcat ttggaccatc acataaagct ttccttgt
//
LOCUS       DAPHDX010000332         1052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPHDX010000332 DAPHDX010000000
VERSION     DAPHDX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.337"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1052
                     /locus_tag="RH058_004623"
     CDS             <1..>1052
                     /locus_tag="RH058_004623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140051.1"
                     /translation="PDFIDSQLAFFNKASSPNVSGDLYPNAPRFSWSTESNKISTVNK
                     NWEGILNANPGAYVTRETISTNVEQYNYNGINGSDIISIKNSLFGLDRTQVDRQPVGL
                     FTGEGNDIIVTGQDYGRNTSLGYTDHANITNMGGGDDTLVVGAGNNNVTLYVNDDGQL
                     RATTDGYDGLYSINYNQINPGVTGGTISGTDISMGEGNDTILALGTEGNTADAIINTN
                     IDLGQGNDFIYINGNVSSAWNTSVNIRGNEGVDTLFIDNGDISSLQFSGFEVIKLGRN
                     GGVNIREVDLLKSGADAIEGGDLKIIREASSRVKLEGDFIQKGIETVEGVNYLVYSND
                     NTPGIRILVDTDIPLI"
BASE COUNT          342 a          153 c          236 g          321 t
ORIGIN      
        1 ccctgatttt attgattctc agttggcctt ctttaataag gcatcgtctc cgaatgtttc
       61 gggggactta taccctaatg caccgagatt ttcttggagt actgagtcaa ataaaatatc
      121 tacagttaac aaaaactggg aaggaatatt aaatgctaat ccaggagctt atgttacacg
      181 agagaccatc agcacaaatg tagaacaata taattacaat gggattaatg gttctgatat
      241 tatttcaatt aaaaactcgt tgtttggatt agacagaaca caagtcgaca gacaacctgt
      301 tggtttattc acaggagaag ggaatgatat aattgttact ggtcaagatt atgggcgtaa
      361 tacatcttta ggatatacag accatgctaa tataactaac atgggggggg gcgatgatac
      421 tcttgttgtt ggtgctggaa ataataatgt aacattgtac gttaatgacg atggtcaact
      481 tcgtgctact actgatggtt atgatggcct gtattctatt aactataacc agattaatcc
      541 tggcgtaact ggtggaacaa tttcagggac tgatatctca atgggggaag ggaatgatac
      601 cattctggct ctgggtactg aagggaatac agctgatgcc attattaata caaatataga
      661 tttgggtcag ggaaacgact ttatctatat caatggtaat gtttcatcag catggaatac
      721 ttcggtaaat atcagaggta atgaaggtgt tgatacactt tttattgata acggtgatat
      781 ttcctcttta caattctctg gtttcgaagt aattaaactt ggaaggaacg gaggggtgaa
      841 tataagagaa gtcgatttac tgaagagtgg agcagatgct atagagggag gtgaccttaa
      901 aataataaga gaagcttcct cgagagttaa acttgaaggt gattttattc agaaaggaat
      961 tgaaacggtg gagggtgtaa actatttagt ttattcaaat gataataccc caggtattag
     1021 aattctagtt gacacagata taccacttat ta
//
LOCUS       DAPHDX010000333         1040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPHDX010000333 DAPHDX010000000
VERSION     DAPHDX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.338"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1040
                     /locus_tag="RH058_004624"
     rRNA            <1..>1040
                     /locus_tag="RH058_004624"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          242 c          329 g          211 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac
//
LOCUS       DAPHDX010000334         1037 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPHDX010000334 DAPHDX010000000
VERSION     DAPHDX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.339"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(222..815)
                     /gene="rclC"
                     /locus_tag="RH058_004625"
     CDS             complement(222..815)
                     /gene="rclC"
                     /locus_tag="RH058_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV3140052.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(827..>1037)
                     /locus_tag="RH058_004626"
     CDS             complement(827..>1037)
                     /locus_tag="RH058_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3140053.1"
                     /translation="TLLSGVMAFSANADDKIILKHISVSSVSASPTVLEDTIADIARK
                     YNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT          299 a          221 c          216 g          301 t
ORIGIN      
        1 cggtaaaaca ttttaatcct gaatttgttc gtggttgtct tgctgtaaaa atacatgatt
       61 gatattaatc atatatttta cctgagacga caagaatctt ttaacagggg agtgatattg
      121 atcttcactc tgtcatatct ccggtaatat ggcgtcaggc ttcatcatca gaaagggggc
      181 ctgacgcctg aaaaagtgaa caacagacag tgttcggatt atcaatattc agtttttaac
      241 gttgaactgg attcattact gcgttgttta agaatttccc gcgccgaatc tgccattatc
      301 atgacagcac ctgccagcat cagagtatct ttcaatacca ggcgaccagc accggataaa
      361 taagggaaac catgatgagc gtcacccaat gcgggtaccc atgcctccgg ggtggtgatt
      421 aaaaatgaga gtgttaccag cggcgtggta aatgccatca gtcctcccaa taaacctaac
      481 cagcgattga caggattagc caaaaccagc agagcaataa tcacctccac gacgccaaga
      541 ccgttggaaa aaccataggt attattggcc gtttgccatg cccttgcttc tggtttgtat
      601 tcgccttcgt gagtcagata ctgtttatag tcttccgggt gttcataaaa gaacgacatt
      661 agtggactgt ttgcgacgaa tggtgtaatg ctgtctgcct cgtaagggac aaactttaat
      721 aacccaatcc acataaaaac aattgcaata ctcagacgaa tcaatgtcag gccaatttta
      781 tctccccgac ttaacaggtg caggtatttt tccattgtga acatccttat ttatacaata
      841 ctgctgttgc ggttgaatta ttatcaattc gcatcgatgt gactttccag gatgaagcat
      901 tatattttct ggctatatca gcaatggtat cctccagaac tgtcggtgat gctgatactg
      961 acgagacgct gatatgtttc agaattattt tatcatctgc attggcggaa aatgccataa
     1021 cgccagataa tagtgtt
//
LOCUS       DAPHDX010000335         1013 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPHDX010000335 DAPHDX010000000
VERSION     DAPHDX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.340"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..921
                     /locus_tag="RH058_004627"
     CDS             7..921
                     /locus_tag="RH058_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140054.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          311 a          184 c          201 g          317 t
ORIGIN      
        1 gataaaatgg ctggtttaag gaataacaac aacacacaga atgcacagtg ggctgattat
       61 gtgggtgaca ttcttcgggg ggctcagccg ataaaccagc tagtacctca gcacccttat
      121 cttaacgacg tcccactcat tgatgagtta aggcatcaga acactcatca tgttattctc
      181 ttgacacttg atgttgcaaa aaaaatactc agcccaataa catcatttga ttacattcat
      241 tttatcacta cacatccttc aggtataaaa gatacactcg cctggttagt taacgcgggt
      301 aaattaatga ctgagtttga tgacaatggg aaaattatat ttaaccttaa tgccttaaag
      361 tatacaaaag caagttactt tgaaatactt ggagagaaat atataaaaat cacaacatcc
      421 agcccctggc ttttagaaaa gctcggtaaa tatattttct cttcacgagc gcctcaagtt
      481 ctggaactcg ctattggctg gcgaggagct ttatctgaat caataaaagg agttaagttc
      541 tgtatctggt tttcagtagc ctggagaacc atagaattta ttatgagttc agagcgagat
      601 ctggttaatt ttcttggcga tttttcaatg gatgtggcca aagcagttat agccggtggt
      661 gttgcaaccg ctattggaag cctggcttct tttgcctgtg ttagctttgg ctttccagta
      721 attcttgtcg gaggagcaat tttactgaca gggatagtgt gtacagttgt tttaaatgaa
      781 atcgatgctc aatgccattt atcagaaaaa ttaaaatatg caattagaga tggactaaaa
      841 cggcaacagg aacttgataa atggaaaagg gaaaacatga ctccatttat gtatgttctt
      901 aacactccac ccgtgatatg aggattaacc atgcgtttac atgttaaatt aaaagagttt
      961 ctttcgatgt tttttatggc catattattt ttccctgcat tcaatgcttc ttt
//
LOCUS       DAPHDX010000336          997 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPHDX010000336 DAPHDX010000000
VERSION     DAPHDX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.341"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..250)
                     /locus_tag="RH058_004628"
     CDS             complement(<1..250)
                     /locus_tag="RH058_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140055.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQPAILSAMSIYK"
     gene            444..869
                     /locus_tag="RH058_004629"
     CDS             444..869
                     /locus_tag="RH058_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140056.1"
                     /translation="MDIEFSQIDEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            905..985
                     /locus_tag="RH058_004630"
     CDS             905..985
                     /locus_tag="RH058_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140057.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          315 a          148 c          179 g          355 t
ORIGIN      
        1 atttatatat gctcatagca gataatattg ctggctgaat tatctcttca gaaatatccg
       61 ctatctttat tgacaaatca tgtgttagat attttcctcc aagagattct tgttcaatat
      121 aatttaaacc agaccccatt gtgattttaa ctggattaac ggatttctct tcatttttct
      181 tatttttgtt ctcctgatca ggaactaatg gttgcagtgg taggctatta ttaatatttc
      241 gtggcttcat tttacatttc tctcatttga ttagagcaaa atccgaattc gcagcttctg
      301 ataatgtcaa atatcacctt atggagacgt tacgtagaat gactatattt gttatcggca
      361 cattaggggt attaatttta gtattgatgg aaggtgatta ttgaacagta acatcgagtc
      421 acggtaaaaa tgtgaggtat ataatggaca ttgagttttc gcagattgat gaaatggtct
      481 atatgcatga tattgtaaat tcggactcga aaaagaaacc aaggattccg ttaaaaaaat
      541 tccttaatgc tgagaaggtt ctcacgcaaa caacatcatg ggcattaaat tcaagatttg
      601 tgaatgttaa ttctgtcaat aaagtcaacg taaagagtaa agttaaaagc agttatatct
      661 cacgatctgt caatgatgag tttagtttaa cagatgatga aatcaattct tttaaagaaa
      721 cgttagtcct ttcctctatt gattcactga gtaaactggt tcttaataat cctctttctg
      781 tattgtttac atcgactgta cgaaggaata ataatcgagc taagatgaat gtcgagttcg
      841 actcatggat ttgcaccaga tgttgttgat acatgatatt ttacattgca aggagatgtc
      901 taaaatgaaa gatgttgatc aaaactttga tgctttagac tgccatatac tgcgagaata
      961 tttaatttta ttgttttatg attaacgagg ttccatc
//
LOCUS       DAPHDX010000337          978 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPHDX010000337 DAPHDX010000000
VERSION     DAPHDX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.342"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            109..954
                     /locus_tag="RH058_004631"
     CDS             109..954
                     /locus_tag="RH058_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140058.1"
                     /translation="MKNWAPEHTKEIKLWLDISTYRKFEDLSLIQFYHELWARNLFFK
                     EYREDFESKELMVYFSKIFSGNPFLIEEGQLGYMAPANKLFQPPHFFLTTLDRLAETS
                     IIAMQRGGFVWHEGDNYSINRELREESLSDIMPDQFSRTVMFEIDLASGTDEEIAESL
                     KTALPQWRKVKGIDENPLESVRFGYGTIKKLISYRVIPMLDILVWAAVKKIRVSDDRL
                     SRLLYTDNDEESEVRLPNQIKDTDRPLALKACTIAFIRQFNHFINKNNHLKQIKVNDV
                     IKLTD"
BASE COUNT          326 a          168 c          202 g          282 t
ORIGIN      
        1 ctaaagacat ttcaactgtg tgtggaaagt cgcataactt gcataagttg agtaagtctg
       61 acaattttaa cactaacaga ttatggattt ttgttggagt gtatacttat gaaaaattgg
      121 gctccagagc atacgaaaga gatcaagtta tggctggata ttagtactta ccgcaaattt
      181 gaagacctct cattgataca gttctaccat gagttatggg ctcggaactt gttctttaag
      241 gaatatcgag aagatttcga gagcaaagaa cttatggtgt atttctcgaa aatattcagc
      301 ggtaatccct ttttgatcga agaagggcaa ttgggataca tggctcctgc caacaagctc
      361 tttcaaccgc cacatttttt tctaaccaca cttgaccgac tcgctgaaac gagcattatt
      421 gcaatgcagc gtggaggatt tgtttggcat gaaggtgata attattctat aaacagagag
      481 cttcgggaag aatcattatc agatattatg ccagatcagt tttcaagaac agttatgttc
      541 gaaattgatc ttgcgagtgg tacagacgaa gaaatcgctg agtcgcttaa aacagctctc
      601 cctcaatggc gtaaagtgaa gggtatcgat gagaacccat tagagtcagt tcgctttgga
      661 tatggaacga tcaagaagtt aatcagctat cgtgtaattc ctatgctgga catattggta
      721 tgggctgccg ttaaaaaaat tagagtttct gatgacagat tatccagatt gttatataca
      781 gataatgacg aagaaagcga agtaagattg cctaatcaaa taaaagatac cgacagacct
      841 ttagcactta aagcgtgtac gattgccttt atcaggcagt ttaatcactt cataaataag
      901 aataaccatc taaaacaaat taaagttaac gacgtcataa aacttacaga ttaaaacagt
      961 atacttcaac cgtagtag
//
LOCUS       DAPHDX010000338          975 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPHDX010000338 DAPHDX010000000
VERSION     DAPHDX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.343"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..206)
                     /locus_tag="RH058_004632"
     CDS             complement(<1..206)
                     /locus_tag="RH058_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3140059.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          271 a          196 c          287 g          221 t
ORIGIN      
        1 tacggacggg actgtgcaat acgcgcctgc agccaggctt ccacttcgct tttcagccat
       61 cgggagctgc ggcccatttt gatgggcgca ggaaagtcac catccttgat gagtttgtaa
      121 aaccacttat cggtcaggcc ggtcagttga gtgataaatg ccatgtcgac catctggtca
      181 tccatcagtg aaactggggt gatcatgaga atgttcctcc ggatgttgtt aaaccagccg
      241 gtgtggtcat gaaaacaacg caccaccttc accggcataa caaccgggga gaaacgtaaa
      301 aaataatcgg gaatgtgacc gccaggagat aaacggggag gttacggtgg ctgagccgta
      361 ccggtatgcc gggtggttgt tacgaaacga tgcttccgca cgcgcgcgcg ataaagaagg
      421 gatgattttc gtgaggaaga ggggaaaact ggcgttctga taattgcaga gttatttacg
      481 gggcggttac gatggggcag accgtgtccg gtgggcaggc gacaggtatc accgatggga
      541 taagactgtc tgattggtat tataaatata taagagagat caaacacatg agatcaaata
      601 catgagttca tatatgtgaa ataaacacgg tgagtgtgat cagtgggcat gatgagaacg
      661 cagtagcaac agactgcgct atccctgata gctggcgtat aacccgcgta tataaaacgt
      721 atctgcaatg tacgtgaaca gagtgcatta agcatccgtc tgagcggtaa acatatccga
      781 cctggtgagt gacagtctga gagaccggtt aagtggtggc tcgggagtaa tgagtttggg
      841 gtgggtacca gaatgccagt ctggcggtat gctgacagta tcacgttaat tgcagcataa
      901 agtgatgaga ggctaatgga agttaaaacc aaagcagttc cgtcgttgtt catcccggga
      961 gacgctggaa aaagt
//
LOCUS       DAPHDX010000339          974 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPHDX010000339 DAPHDX010000000
VERSION     DAPHDX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.344"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          288 a          191 c          199 g          296 t
ORIGIN      
        1 tagcaaaaaa aaataacacc catcattatt aatgtttgta aagagcccac gttttcagtg
       61 ggctgattag tccaaaagct gaagttcttg gtgctgaata aatcctgaat gaattttcct
      121 tgtagtggtc aataaaaacc gaactccatt ttatttttta tttctctgca cacctcagtc
      181 agtttgatca gctgctgaaa aaactcagcg ttgaagccgc tttcaatgca gaaatggcac
      241 accatctcgg gtatgaaaaa aatcagcccc gtattaccgg gacggataac tgcaagtatc
      301 ccgcataatc gtgccattca tatttagaaa tcatccggca taatcaatct gccaacgaag
      361 gagatcgcta tgcgtacagc ccgttttact gagcatcaga tcatcgctgt gattaagtcg
      421 attgaagccg gacgaattgt taaagatgtt tgctgacctg agcctggaga accgagtgct
      481 gaaggatgtt atcgaaaaag ctttaaaaca agcctgtaag cgtgagttgg ttactcatct
      541 gataacgaca ttcgggctca gcatccgtca ggcctaccag agtttgaacc tgagcagaac
      601 ggcttaccat taccgcccag ataccacgcg tgatgaaccc gttataaacg gggctattta
      661 tatgcttacc ttttgtgtat taccctcaat attaaattaa tgttgagggt atttgggtgc
      721 gatattgttt tttttgaggc gtaattttac agtattattt ctgaaaaaat atttgcgtat
      781 gctatttaaa gcaaaaaaaa tcttcagtgt aggcaataaa attattgttc ctttcgcact
      841 tctggcatag caatgttacc gccatactgt tcttccagta atgatgcaag aaatttaaaa
      901 gatttttgtt gttctggcga ccatttgaca ttaagtgact cgaaatggat tgatgccagt
      961 gttggtagca tttg
//
LOCUS       DAPHDX010000340          962 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPHDX010000340 DAPHDX010000000
VERSION     DAPHDX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.345"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..606
                     /gene="cas1"
                     /locus_tag="RH058_004633"
                     /pseudo
     CDS             82..606
                     /gene="cas1"
                     /locus_tag="RH058_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            603..896
                     /gene="cas2"
                     /locus_tag="RH058_004634"
     CDS             603..896
                     /gene="cas2"
                     /locus_tag="RH058_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV3140060.1"
                     /translation="MSMIVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT          245 a          187 c          262 g          268 t
ORIGIN      
        1 gtgacttttt ttgatttctg gggaaaaacc cgacgcggcg agaaagacgg gggcgacgac
       61 tatcaccttc tttgctggca ttcgctggat gtacggggaa ttgagggatc ccgcgttcgc
      121 cagacgtatg cattactggc gaaacaatat ggtgtgaaat ggaatggtcg caaatacgat
      181 cctaaagact gggaaaaagg cgatgttgtc aatcgttgca tcagtgctgc cacatcatgc
      241 ctgtacggta tttctgaagc tgcagtatta gctgcgggat atgcgcccgc tattggtttt
      301 atccatagcg gcaaaccgct ttcatttgtt tatgacatag ccgatatcat taaatttgat
      361 ttggttgtgc caaaggcatt tgaaatagca gcgaggcaac ccgcagaacc agataaagaa
      421 gtcaggttgg cctgtcgcga tattttccgt agtagtaaat taacgggcaa gttaattccg
      481 ttaattgagg aagtgcttgc cgctggtgaa attgaaccac cacaacccgc gccggatatg
      541 ttaccgcctg ccatccctga acctgaaacg ttaggcgata gcggtcatcg aggacgcggc
      601 ggatgagtat gatcgtagtt gtaacggaaa atgttcctcc acgtttacgt ggacggctcg
      661 ctatctggct actggaagtg cgtgccggtg tgtatgtcgg agatacatcc aaacgtattc
      721 gggagatgat ctggcagcaa atctctcaac tggcaggttg cggaaatgta gtgatggctt
      781 gggcgaccaa taccgagtca ggttttgaat ttcagacctg gggtgaaaat agacgtattc
      841 cggtggattt ggatgggttg cgtttggttt cttttcttcc tgttgataat caataagtta
      901 tccgttcttt aaaaataagg aaatgtttta atttagttgg tagattgttg atgcggaata
      961 aa
//
LOCUS       DAPHDX010000341          952 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPHDX010000341 DAPHDX010000000
VERSION     DAPHDX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.346"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..364
                     /locus_tag="RH058_004635"
     CDS             <1..364
                     /locus_tag="RH058_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-dependent DNA polymerase"
                     /protein_id="HDV3140061.1"
                     /translation="LSTELNLAYVARMLSKENSEKSTLILSEIYNNNPESILVKNIVT
                     QSMAKINSYAWLSDIKKNFSAMHPLQRRLLIVSSYILGDEGRHWREHNKKTFNFVEVI
                     YRDWASKRHTARNLEDAL"
     gene            361..>952
                     /locus_tag="RH058_004636"
     CDS             361..>952
                     /locus_tag="RH058_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140062.1"
                     /translation="MISELTFSRKFTSFWNQLLPNANNFIRIINGSLIEDVYPPLDDC
                     ANRSNNVFVNECAFNLYRAIQNDSLDRNILSAHDIFHNADFQVVFEKTKEYLQRFAYG
                     SNFKLPLSMVEYNAIREIARNILSRYGMENQIEVSPQFDGCGVINNSYGDIYYSNVLV
                     EIKSGDRKFSVYDLRQVLIYFTLNFYSKNKRNIKRFE"
BASE COUNT          332 a          163 c          174 g          283 t
ORIGIN      
        1 tttgagtact gagctcaact tagcctatgt agcccgaatg ctctcaaaag aaaattcaga
       61 aaaatccacc ctaatcctta gtgaaatata caataacaat ccagaaagca tcttagtcaa
      121 gaacatagtt acacagtcaa tggcaaaaat taattcttac gcatggcttt ctgatatcaa
      181 aaaaaatttc tctgcaatgc atccgttgca gagaagacta ttgatcgttt ccagttacat
      241 cttaggtgat gaaggacggc actggagaga gcataataag aaaacattca actttgtaga
      301 ggtgatttac agggattggg caagtaaaag gcataccgca agaaatcttg aggatgcgct
      361 atgatatctg aattaacgtt ttctcgaaaa ttcacttcat tttggaatca attgcttcca
      421 aatgctaata atttcatacg catcattaac ggcagtctca tcgaggacgt ttatcctcct
      481 ctagatgact gcgctaatag gtcaaataac gtctttgtta atgagtgcgc atttaattta
      541 tatagggcaa tacagaatga ttcgttagac agaaatattc tttcagcaca tgatatcttc
      601 cataatgctg attttcaggt tgtttttgaa aaaacaaaag aatatctaca gcggttcgct
      661 tacggttcta acttcaagct acccttaagc atggttgagt acaatgccat aagggaaata
      721 gcaagaaaca ttttgtctcg atatggaatg gaaaaccaaa ttgaagtgtc tccacaattc
      781 gatggatgcg gagtaataaa taattcatat ggcgatattt attattcaaa tgttcttgtg
      841 gaaataaaat caggagatag gaagtttagt gtttacgatc ttagacaggt gctaatatat
      901 ttcactttaa acttttactc aaaaaacaaa agaaacatca agagatttga gc
//
LOCUS       DAPHDX010000342          936 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPHDX010000342 DAPHDX010000000
VERSION     DAPHDX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.347"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(166..729)
                     /gene="ipbA"
                     /locus_tag="RH058_004637"
                     /gene_synonym="fimX"
     CDS             complement(166..729)
                     /gene="ipbA"
                     /locus_tag="RH058_004637"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDV3140063.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          285 a          173 c          182 g          296 t
ORIGIN      
        1 cactggtatg atctttgtgg ttaatcattt caaatcatgg ggttatttta ctttagcgag
       61 aaatatcaac atgttatgtg ttttatttaa ttgaatgggc tgttttttta tggaagaatt
      121 gaggaaaacg taggaatctg acattttgaa tcagaaggta ctgttttaac gaggcttttt
      181 tttccacacc cctttaaaac gagcagcatt actggctgtg tatctgacag tatgctgaat
      241 atttcgatga cccagataat cctgaagtag tcgggtatcg acaccattgt cagccagagc
      301 aaaaccacag gcatgacgta acatatgagg atgtgctttt acagctaatc ctgcacgttt
      361 tccagcctca cgaatgatag aaaaaaaatg ttgtctgctg atagggcggc gtttccggga
      421 gagaaaaagc cagtctcctt caactccgtt ttcaattaac ttcctctgct taagccacaa
      481 cttgatcaaa ttatattcat caggaagaag aggatgtgtt gtcgagaaac catttttaat
      541 acgccgaata ttaagttgtt ttccagatgc atcaatatcg gataaacgta aatcaagaag
      601 ttcactggcc ctaaaaccgt gaatgaaagc catcataatt agacagcgat ttctttcagg
      661 aaaggacata ctctgggctg catccatcag cctgtagact tcatcctgtg tgagatattt
      721 tctcgtcata cgccaccgtg tacagtttta aaaaaaagag aggcacaccc agataagtat
      781 acctgcaatc agaacgttat cgatgtaatc tcattatctc attattaaga ttttttgaat
      841 agtagttcta cgcaacagat taaatatgaa ctatttattg tgcagctttt ctacaagtca
      901 tttttaatgc gtgtttttat ctacagtgat gtgaga
//
LOCUS       DAPHDX010000343          931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPHDX010000343 DAPHDX010000000
VERSION     DAPHDX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.348"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..318
                     /locus_tag="RH058_004638"
     CDS             <1..318
                     /locus_tag="RH058_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDV3140064.1"
                     /translation="FSQGFIGSELFISDSEFRGKLNEKLPAHIKLADHINRPDAKKYK
                     LVFAIASNKNLPEDLPLFSKVNLKNFHKSITNFGYEVRICKIAVDPDIYKKKLCKPQK
                     NKL"
     gene            complement(478..690)
                     /locus_tag="RH058_004639"
                     /pseudo
     CDS             complement(478..690)
                     /locus_tag="RH058_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          298 a          176 c          159 g          298 t
ORIGIN      
        1 ttttcacaag gatttatagg ttctgaactt tttataagtg actctgaatt tcgaggcaaa
       61 cttaatgaga aactacctgc tcatataaaa ttagccgatc acataaatag accagatgct
      121 aaaaaatata aactagtttt cgccatagct tctaataaaa atcttcccga agatttacct
      181 ttgttttcta aagtaaactt gaaaaatttc cacaagagta ttaccaattt tgggtatgag
      241 gtcaggatct gcaaaattgc tgtggaccca gatatttata aaaagaagct atgtaaacca
      301 caaaagaaca agctttaaat aaatttttca atgccagaga cgtttgccag ttcatgcaga
      361 aaatcgcttt tgtaagttac agtccatgta tcttggttta gaacaaagtg gtgtcaaaaa
      421 aggtttatgc cttgggaggg aataaggggc tgggaaattt aaccccttat agagttgtta
      481 tgccgcaata gctttaaatt tttgagcaat gacataatca ctccaccact gcatgagaac
      541 cattcgttct gcgagatact cggcacgatt ataggctgca attatttaat ctttcttcga
      601 gtgggcaagg gccgcttcta agacaccagt cctgaactta ccagattcct ctgtcgctgt
      661 tctcgcaata gatctcattc tgttaaccat aagttcacct ccttaaaccg ttcgaataat
      721 tacggcttat actgcctact tatgctaata tctaagagaa gccattgtgg ttgtgtaagt
      781 atacttcttg gtttatttcg actatagaaa accataccta tgactggctg ttttcagcaa
      841 gagtcttatc atctaaagaa tttattttcg aaattctttt taactcatta agataatcaa
      901 cctcgttttc caagaaccaa tgaagaactt t
//
LOCUS       DAPHDX010000344          928 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPHDX010000344 DAPHDX010000000
VERSION     DAPHDX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.349"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..820
                     /locus_tag="RH058_004640"
     CDS             35..820
                     /locus_tag="RH058_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140065.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSFDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          306 a          188 c          204 g          230 t
ORIGIN      
        1 accatgatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat
       61 atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttcttttg atgaaaagaa
      121 gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc aactgacaga
      181 caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata ttcaagctgc
      241 gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg ttcaatccgg
      301 caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata ctgtttccac
      361 attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa
      421 tgagaacgag gatgttgcca gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt
      481 ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatacattgc agacaggaaa
      541 atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa aactcaccga
      601 cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa caggtgagtg
      661 ggctacctgg tcgccggtga attatgaata taaagtgctt ccaccattgc ccgacaagaa
      721 tgaagccagt acgactgata tgacagagca gcaaatcatg caacttaagc aaaagaccta
      781 taaagcgatg gtaaaagatt tggttaaccg ctatcaataa taaattatat ctgccgccag
      841 gaatttctgg cggcaataat acaaaattct ttgcataaga aatttttctt gacaaagaca
      901 gtgcaagaaa ggacatggaa tccggagt
//
LOCUS       DAPHDX010000345          924 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPHDX010000345 DAPHDX010000000
VERSION     DAPHDX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.350"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..552)
                     /locus_tag="RH058_004641"
     CDS             complement(220..552)
                     /locus_tag="RH058_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV3140066.1"
                     /translation="MAGWFELDKSSNEQYKFVLKAGNGEIILTSELYTTKSSAENGII
                     SVQANSPLDERYDLKDSSNGKSYFNLRAANHHVIGTSQMYSTKQSREKGIASVKTNGT
                     TTTIKDNT"
BASE COUNT          273 a          162 c          190 g          299 t
ORIGIN      
        1 tatcggttca atggagaaat agctttcata atgtcaagga tttcgaatat aaagaattgt
       61 ttaaagagta cgctaacagt aaaatagatg aaatcatatc atcaaactct aaataatgat
      121 tcggaatcat ttctaccgtt ggctggggta cataaaagcg taatattttt attcattctg
      181 ataaagccaa gtcacatggc ttggcttcag atatattgat taagtattat ctttaatcgt
      241 tgttgttgtt ccgttggttt tcacggaagc gatacctttt tcacgggatt gtttggtcga
      301 gtacatctgg cttgtaccga tcacatgatg attagccgcc cgtaggttaa aataggactt
      361 gccgttagat gagtctttca gatcgtagcg ttcatcaagt gggctgtttg cctgaactga
      421 tataatgcca ttttctgcag aactcttggt tgtgtaaagc tcgctggtga ggataatttc
      481 tccattaccg gctttcagaa caaatttata ctgttcatta ctgctcttat ccaattcgaa
      541 ccaaccagcc atattaactc cttgataaag ttgtgatttt tacggcccag tgatgaagaa
      601 cgaagcgggc aagttcagta tgcctgaaac aaaaattgtt tccaatatgt tatgtttgta
      661 tcggtagctg ggaacaagac tacacactac gaaatgaggc ttagctgtcg cccaccagtg
      721 ttggcaggta tgccttagtg ggagtcttag agcggagggt atcttgaatc aatgtctatt
      781 gccatattta cacgacacac gatatatttt ctttagagtc atatcttagg aaacaataac
      841 ggcgacttga gcaaaagtat ctaatatcaa tagattttcg ttaatttaaa aaaataggtg
      901 ctctctcata aaatctgtag ggta
//
LOCUS       DAPHDX010000346          920 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPHDX010000346 DAPHDX010000000
VERSION     DAPHDX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.351"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            88..870
                     /locus_tag="RH058_004642"
     CDS             88..870
                     /locus_tag="RH058_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV3140067.1"
                     /translation="MKISDFICSPLGWNESLPFYLRDNFLLSQNINSVDDVCVVDPGS
                     KIMGIDYLVPKFLDEMEKVDWEKMGLTFCDKKEFSSSEYERLLSKIESLLYFSKNMHS
                     SIKAFAINFHPLLSECEDFDVSFSDPCIPFDVFTNVPKYEEKYAAERFVEGIIHETLH
                     LQLTAMEHLLPLFHDTLLTDNVFSPWKGEGRNERGLLHAVYVFSNIKNFWLKVHAQSN
                     HINDFAVKRINEIQKELSTAGHILKSDALTPEGRMLACLCLL"
BASE COUNT          303 a          129 c          182 g          306 t
ORIGIN      
        1 agatatcaca gaactcacat catgcggtta gtcaggtata cgattttgaa agatatactg
       61 taatttactc attttacagg aagggaaatg aaaatatctg attttatatg ttctccttta
      121 ggttggaatg aaagcttacc attttattta cgtgataact ttctgttatc acaaaatata
      181 aactccgtag atgatgtatg cgtagtagat cccggcagta aaataatggg gattgattat
      241 ttagtaccta agtttttgga tgaaatggag aaggtagatt gggaaaaaat gggattaact
      301 ttctgcgaca aaaaggaatt ttcgtcatca gaatatgaaa ggttattgtc caaaattgag
      361 tctttattat atttttcaaa aaacatgcat agttcgataa aggcgtttgc aattaacttc
      421 catccattat tatctgagtg tgaagatttt gatgtgagtt tcagtgatcc ttgcatacca
      481 ttcgatgtat tcactaatgt cccaaaatat gaagagaaat acgctgctga aagatttgtt
      541 gaagggatta ttcatgaaac tctgcattta cagttaacag ctatggagca tttattacct
      601 ttgttccatg atacattgtt aactgataat gtattttccc cctggaaagg agagggccga
      661 aatgaacgtg gtttactaca tgcagtctat gttttctcaa atattaaaaa tttttggttg
      721 aaggtgcatg cgcaatctaa tcatataaat gactttgcag taaaaaggat taatgaaatt
      781 caaaaggaac tatcaacggc aggacatata ttaaaaagtg atgcattaac tcctgaaggt
      841 agaatgcttg cctgcttgtg tttattataa ccactcagct atgatgcaag gtcataatta
      901 taggggattc tttgagggtg
//
LOCUS       DAPHDX010000347          919 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPHDX010000347 DAPHDX010000000
VERSION     DAPHDX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.352"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(197..694)
                     /locus_tag="RH058_004643"
                     /pseudo
     CDS             complement(197..694)
                     /locus_tag="RH058_004643"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          241 a          209 c          207 g          262 t
ORIGIN      
        1 ctcagacgtg atgagtatat cattggcgtt atttccatag ccggagatcg gtgagttacg
       61 gcgtttttca atatggttgt cgcctgatgt aacaaaatta cgttatgccg cttctttttc
      121 tggattcagg tagacttctg atatatagcc cagttacgta gatgcttgct ccaacgttca
      181 ggatgttgct cgcgtgctag ctgttatacc acttttctga ctcggctggt gtgacatatt
      241 ttatcccacc gtgcttatgt tcaaagttgt accagtttac aaaaccgttt acccatactc
      301 tgacttcatc aatagtacaa aaaccgttct caggccacca gggacaatat ttgagagtcc
      361 ggaacaaaga ttctgagtaa gggttatcat tgctgactct tggccggaaa taagaactta
      421 gcacaccaag cgcatacatt ttcgccaaca acgtgcaact gcgcatggga ccaccgttat
      481 cagaatggag taccagattg ttggatgaac atttttcatt ccatactgca cgctggagta
      541 gttctgctgc atgttttcca gactcgctgg caaatacctc tgcagcaata attttgcgac
      601 tgtaaatatc gacgatcata tagaggtaaa aatgctggcc tctgacccag gacggcaaat
      661 agctgatgtc ccaactccag acttgattcg gcgcaacggc cctgagtaca tcaggccgct
      721 tatactggca ttaacgggat cttttcgtca actggttggc tgatttcagt acacgataaa
      781 agtttgattc agaagcaata taaattcctc tgtctgccag agttggtacg atcacatttg
      841 gaggtaagga agcgaactct ggcgattaca atgtagcata gcgccaggtt atcgctcgcc
      901 acgggaatat ctgcggcag
//
LOCUS       DAPHDX010000348          918 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPHDX010000348 DAPHDX010000000
VERSION     DAPHDX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.353"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..498
                     /locus_tag="RH058_004644"
     CDS             <1..498
                     /locus_tag="RH058_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140068.1"
                     /translation="DIKTSPQLLGLKDNVYFCKIDSSQMLFPNQVGVGLTQIAPLIIA
                     ANIVQDGLIAIEQPELHIHPALQLAVGDLFTQYPLDVKRPMFLVETHSEHILLRILKR
                     IRQTTDNELPESNYPVKPDFISVIVFEDNNGSTVTRKIDITDDGDFKQKWPKGFFEER
                     RGELF"
     gene            498..>918
                     /locus_tag="RH058_004645"
     CDS             498..>918
                     /locus_tag="RH058_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140069.1"
                     /translation="MFYECAISPEALFEIAIDRRNYRDFIKGFSTGGNFLYSELPKLK
                     RNKKQLLGLLNANHSELQKKRLEDLIIFLKNNKVSRVYDYVGDMSWSDNISAVNRIEQ
                     FDHVVSSTPCDNLDVTNIDDFFGLNYARQKIVARIAED"
BASE COUNT          305 a          146 c          173 g          294 t
ORIGIN      
        1 gatataaaaa cctcccctca acttttaggt ctgaaagata atgtttactt ctgtaaaata
       61 gattcaagcc aaatgttatt tccaaatcaa gtcggtgtcg gattaactca gatagcacct
      121 cttattattg cagccaatat agttcaggat ggattaattg ctatagagca gcctgagcta
      181 catattcatc ccgctttaca actcgctgta ggtgatctgt ttacccaata tccccttgat
      241 gttaaaagac ctatgttttt agtcgaaaca catagtgagc acattttgct tcgaatttta
      301 aaaagaatta gacaaacaac ggacaatgaa ttacctgaat caaattaccc agtaaaacct
      361 gattttatat cagtgatcgt tttcgaggat aataatggtt cgactgttac taggaaaata
      421 gatattactg atgatggtga cttcaaacaa aaatggccca aaggtttctt cgaagaaaga
      481 aggggagagc ttttttaatg ttctacgaat gcgccatttc accagaggct ttgtttgaga
      541 tcgcaattga tcggagaaat tatcgtgact tcataaaagg attttctacg ggcggtaact
      601 ttctctatag tgaattacca aagctgaaga gaaataaaaa acaattgctt gggctattaa
      661 atgctaatca tagtgaactt caaaaaaaac gattagagga ccttattatt tttttgaaaa
      721 ataataaggt tagtagggtt tatgattatg ttggtgacat gtcatggtca gataacatct
      781 ctgctgtaaa tcgaattgaa caatttgatc atgtagtgag ttctactcct tgtgataacc
      841 ttgatgtaac taatatagat gatttctttg ggttaaatta tgctcggcaa aaaattgttg
      901 ctagaattgc tgaggata
//
LOCUS       DAPHDX010000349          916 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPHDX010000349 DAPHDX010000000
VERSION     DAPHDX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.354"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..861
                     /locus_tag="RH058_004646"
     CDS             <1..861
                     /locus_tag="RH058_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HDV3140070.1"
                     /translation="VINVEGHQEGASKGYNPKKLGNRCYNIQFAFCDELKAYVTGFVR
                     SGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIETIESLGCKYLIKAKS
                     YSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRVLKPEKERAQLSLL
                     EGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGHLLLKSFWANEAV
                     FQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYP
                     YKGVYEKCLV"
BASE COUNT          336 a          140 c          176 g          264 t
ORIGIN      
        1 gtaattaacg tagaaggtca tcaagaaggt gcgtcaaaag gatataatcc taagaaactg
       61 ggaaaccgat gctacaatat ccaatttgca ttttgcgacg aattaaaagc atatgttacc
      121 ggatttgtaa gaagtggcaa tacttacact gcaaacggtg ctgcggaaat gatcaaagaa
      181 attgttgcta acatcaaatc agacgattta gaaattttat ttcgaatgga tagtggctac
      241 tttgatgaaa aaattatcga aacgatagaa tctcttggat gcaaatattt aattaaagcc
      301 aaaagttatt ctacactcac ctcacaagca acgaattcat caattgtatt cgttaaagga
      361 gaagaaggta gagaaactac agaactgtat acaaaattag ttaaatggga aaaagacaga
      421 agatttgtcg tatctcgcgt actgaaacca gaaaaagaaa gagcacaatt atcactttta
      481 gaaggttccg aatacgacta ctttttcttt gtaacaaata ctaccttgct ttctgaaaaa
      541 gtagttatat actatgaaaa gcgtggtaat gctgaaaact atatcaaaga agccaaatac
      601 gacatggcgg tgggtcatct cttgctaaag tcattttggg cgaatgaagc cgtgtttcaa
      661 atgatgatgc tttcatataa cctatttttg ttgttcaagt ttgattcctt ggactcttca
      721 gaatacagac agcaaataaa gacctttcgt ttgaagtatg tatttcttgc agcaaaaata
      781 atcaaaaccg caagatatgt aatcatgaag ttgtcggaaa actatccgta caagggagtg
      841 tatgaaaaat gtctggtata ataagaatat catcaataaa attgagtgtt gctctgtgga
      901 taacttgcag agttta
//
LOCUS       DAPHDX010000350          915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPHDX010000350 DAPHDX010000000
VERSION     DAPHDX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.355"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..915
                     /locus_tag="RH058_004647"
                     /pseudo
     CDS             1..915
                     /locus_tag="RH058_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959889.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
BASE COUNT          322 a          147 c          181 g          265 t
ORIGIN      
        1 tggaggatgc acgagcatat ctatatgtaa aggagtacat ggacccaaaa cctcctgaat
       61 ggaccacctt ttttatggac caaaatcctg gcatagaacc tgatttcttc ggtatgaata
      121 gtagcacagg agcagtatta gttattgaag ctaacaattc cagatatctt attccctttg
      181 gaaccggtca tcacttaatc aatgatggca gcattgtcaa aggatttggg cttaaaacaa
      241 ctcttaatag tatagaacat aaaaaaattc gcagtttaga taaaggcagt cacaacgaaa
      301 cgaacctgtt aactcgcagt cagagcagta aagaagtcga tatatacaac ctaaaaatag
      361 attctgagat ggatattctc acaactctca caggcacttc gacagaagat attttaggaa
      421 gtaaaattac tggcaaagat gcttttgtga tcatgccgga tattgaattg aaggctattc
      481 cagcactatt gaccaaaata gactctattt acttattacc tcttcctgaa gaattcgaat
      541 gggttaacaa tatcaaggag gctgatgaac aagaagttga gatattggat tcgcttttga
      601 ttgatcggat aaaggaaaaa gacttcaatg atttatggct tggagagcca gaaatagtag
      661 actgggaaaa tcaaataggc tattgctttg ataagagaca aagattcatt catgaaacct
      721 tatcaatata ccatgtttgt gattattttg atggtaagaa aactgaagta actgttgatg
      781 aactcaaaaa aattatctgc atgttttgaa tgccgatttt gaatcgataa aaaaatggtc
      841 tttatatagg tgcctctatg ccgagataaa agaaggtgat cagaactata ttcttcgaga
      901 ctctatatgg tatgt
//
LOCUS       DAPHDX010000351          911 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPHDX010000351 DAPHDX010000000
VERSION     DAPHDX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.356"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..203)
                     /locus_tag="RH058_004648"
     CDS             complement(<1..203)
                     /locus_tag="RH058_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3140071.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYY"
     gene            complement(215..>911)
                     /locus_tag="RH058_004649"
     CDS             complement(215..>911)
                     /locus_tag="RH058_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDV3140072.1"
                     /translation="DENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTP
                     TSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNV
                     IGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPIS
                     NMQGTPEKPSDIR"
BASE COUNT          251 a          206 c          164 g          290 t
ORIGIN      
        1 cagtaatatt gatatagtcg aaatgatgcc tctgaatcac catgttgtga tttatacttc
       61 agtttcatca gcttttcttc acttaaatca taggtcattg ctgtagatcc catcacactt
      121 tcgttttctt ttattttcac atcagcacca tgcaattgac caataagaga aaaataaaaa
      181 aatagtaaca agcatgcttt catacatcta caacctatct tatatcactg ggtttttctg
      241 gcgtaccttg catattagaa atggggtgcc cagatggccc atatgttatc caattaccaa
      301 ggtcttttgt tgttatagcc attagatcct tatttgaatt aaggttcgtt ggtgctgtta
      361 gcactgtatc catttttaac ttaccattag ttcctcgccc ccaccacgtc catagcgtgt
      421 caggtcctat tacatttgta tgtaactctt tagctaattg gctagcaaat gaattttgtc
      481 ctttacctgt attacaagaa aataaccaaa ctgtcatccc atctttataa ttcccatcaa
      541 atttaattag atatgctaga tctttagctg tcatgatact gcgcgttgcg gattcaatag
      601 atgtgggggt accatgcccc ccgattgtga aaacgcctgg gatattaatc tcatcagcta
      661 cagaatggat aagatcactt tcggggtata aatttatatc aaccaaacct tgcggatcta
      721 catttatgac cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      781 gatcctgagt gatataccgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
      841 ggccggactc ctcatcatac tgctgacccg gcaggcgaat gagctgctgc agatgatgcg
      901 ggttctcatc a
//
LOCUS       DAPHDX010000352          903 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPHDX010000352 DAPHDX010000000
VERSION     DAPHDX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.357"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..>903
                     /locus_tag="RH058_004650"
     CDS             43..>903
                     /locus_tag="RH058_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140073.1"
                     /translation="MPTISSSKVPSPKSWDEFEEITLSAAKLRWNSTDFYRHGRQGQK
                     QDGVDIWGYDGKRHIGVQCKNTTGELGLAVVETEIANAEKFMPKLDHLYIVTTAPRDA
                     KLQKSVREISQKHSKAGDFRIDVLFWDDICNDLAKDEDVFFGHYPQFKSDDNPGKKHD
                     IALWSELTTLLRSDGVIGFLDRTNMAGFSFRESQLEPIREFYFEWNNPEHEFITPELE
                     KINKELWSEVDRYYNLIANETFPANNPQFSTVPPEWEFEQPDRFWRVVNALHDHAGKI
                     VKLHADIVRTG"
BASE COUNT          280 a          178 c          210 g          235 t
ORIGIN      
        1 atgatataaa cataaaacca aacaaacttt catgagaaaa taatgcctac aatctcgtcg
       61 tctaaagtac catccccaaa gtcttgggat gaatttgaag aaattacctt atccgcagcc
      121 aaattgcgtt ggaactctac tgatttttat cgtcatggac ggcagggaca gaaacaggac
      181 ggtgttgata tctggggata tgatggtaaa cgtcacatcg gagttcaatg caagaatacg
      241 acaggcgagc tgggtttggc tgttgttgaa actgagatag cgaatgctga aaaattcatg
      301 ccaaaactgg atcatctcta cattgtcacg acggccccaa gagatgcgaa acttcaaaaa
      361 tcagtaaggg aaatctccca gaaacatagc aaagctggag atttcaggat cgatgtgctt
      421 ttctgggatg acatttgcaa tgatttggca aaggatgaag atgtattctt cgggcactat
      481 cctcaattca agtctgatga taatccgggg aaaaagcatg acattgcatt atggagtgaa
      541 ctcacgacgc ttcttcgctc tgatggtgtg attggcttct tggatcgtac aaacatggcc
      601 ggattttcgt tccgtgaatc acagcttgaa ccgattcgtg aattttattt cgagtggaac
      661 aacccggagc acgagttcat tacgcctgag ttagaaaaaa ttaataagga actttggtca
      721 gaggttgata ggtactacaa cctcattgcc aatgaaacct ttccagcaaa taatccacaa
      781 ttcagtacag taccccctga atgggagttt gaacaaccag ataggttttg gcgggttgtg
      841 aatgcgctac atgatcacgc tgggaagatc gtaaaactgc acgcagatat tgtacgtacc
      901 ggg
//
LOCUS       DAPHDX010000353          899 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPHDX010000353 DAPHDX010000000
VERSION     DAPHDX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.358"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..328
                     /locus_tag="RH058_004651"
     CDS             <1..328
                     /locus_tag="RH058_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140074.1"
                     /translation="NNMAIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYYLLDK
                     ENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMK
                     ITGTPE"
     gene            340..789
                     /locus_tag="RH058_004652"
     CDS             340..789
                     /locus_tag="RH058_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140075.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          302 a          106 c          226 g          265 t
ORIGIN      
        1 gaataatatg gcgatatatt ggaatagtag agcatcttta ttttgttcaa ctgaattaaa
       61 ctcgaaatca caatcgcctg cattaggttt gggacatgag tttgctcatg cgcagtatta
      121 ccttcttgat aaggaaaatt tcatggctct tttaagccga acagataaga aatatgagaa
      181 taaagaagag gcaagggtta ttacaataat tgaatcgcgt gcggcgaaaa ctttaggtga
      241 gtgtaccaga ggagctcata gtggactgcc tttttatcgg gtggatggac cgttgcagac
      301 gatgaaaata acgggtacgc cagaatagtg aggtttttta tgatgaaata tttgatggtt
      361 ttattgtctc tattttctgg ttcggtactg gggatggggc gagtgaatga attgtgtgga
      421 atagatagcg tgaaaacgat agagataatt aatcttccat cgtatgttac cacgttagtt
      481 ccgcttagca aggaaggtct taatgaaata tacagatata aagtggtggt gaatgaaatt
      541 agcgatcttt atgctggtaa gataattgat ttattgcaaa tgaagtattt cagaaaagaa
      601 aaatataaca atataagatg gggggttagt atcatttcga aaggtaataa taaatgtgaa
      661 atttattttg atgcttttgg tgaatgtgga agtgtgaatg gcattaacgt gtgttttgaa
      721 aaaaatgaaa tgataggttg gattaaaaaa gaaatccctt tgctgtcgca aaaaataggt
      781 gggctttaaa tggtagggca atattcaaag acagtatagg taactatttg tatggccatg
      841 taaggtaaaa gaaaaggaat aaaccatgag cggaaaaccg gcggcgcggc agggcgaca
//
LOCUS       DAPHDX010000354          896 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPHDX010000354 DAPHDX010000000
VERSION     DAPHDX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.359"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..734
                     /locus_tag="RH058_004653"
     CDS             102..734
                     /locus_tag="RH058_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3140076.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          297 a          157 c          169 g          273 t
ORIGIN      
        1 aatcgcaatt tttcccagca aaattacaaa atcatggtgt attgacatca tcaatcacca
       61 atgaaattat gcaattatat acggataggg aggttcttaa catggggctt tgtagtcgtt
      121 ataaaagtct tacatgcaat agttgctcaa tgcattgcca aataatgcca gaagagtcac
      181 cgcgtttgca gtactgcgca aactcgtgtt tttgtatgtg gcccgaagaa agttcatatt
      241 ttaatcgtgg tgtggtagaa ggtatcttaa caaaaaacca caatgcgaga ttaagcggat
      301 atatttttgt cgatttttca gtaagttttt tacgtctatt ccttgaaaaa gactggattg
      361 actatcttgc aagtactgac atggggattg ttttagtcag cgaccgcaat atgcaatcgt
      421 tagctaatta ctggcgaaaa cacaactcag caatatctgc tgtcatatat aatgatgatg
      481 gtcttgatgt tgccaatgaa aagatcagac aactatttat tggtcgttat ttatcattta
      541 ccagaggtaa cacattaact cagatggaat tcaccataat ggggtatatg gtttctggtt
      601 ataatccata tcagattgct gaagttctgg atatggatat ccgtagcatc tacgcgtaca
      661 agcaacgaat cgaaaagaga atgggtggta aaataaacga attatttatt cgttcacatt
      721 cggtccaaca ttgatactca acgaccagcc agaatcatac tctgtttata cgggcattta
      781 ttgaaatgtc cgtattttat aaataggccg tcccctcaag agtaaacacc atagtattta
      841 aaaccatcac acgaaaaatt cagaagcatt acgaaatatg aatttacaaa caatag
//
LOCUS       DAPHDX010000355          895 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPHDX010000355 DAPHDX010000000
VERSION     DAPHDX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.360"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..643
                     /locus_tag="RH058_004654"
                     /pseudo
     CDS             11..643
                     /locus_tag="RH058_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(662..895)
                     /locus_tag="RH058_004655"
                     /pseudo
     CDS             complement(662..895)
                     /locus_tag="RH058_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
BASE COUNT          295 a          137 c          177 g          286 t
ORIGIN      
        1 ggatgaatat atgggaaaaa ttaaaattgt agtttcagat cagcagccgt ttatgattga
       61 tgggataatt ggatttctcg gacattatcc tgatttatat gaggttgttg ggggctataa
      121 agatctgaag aaatctatag cggagtgtaa caaatctaca gcctaaatat ttattttagg
      181 agaatttgca ggtggaatga tggggtccga actcgtaaaa tgggttaaat cgcataagat
      241 agatgctcat attattacat ttgtagcaaa gatgccgtat attgattcaa taaaattgct
      301 tgaagcaggt gcgaaaggat gcgtatggaa aaccagtcac ccggcgaaac taaatcgtgc
      361 tattgattcg attagtaatg gttacactta ttttgatagt gtacatatgg attgtgaaaa
      421 aatatcttcc aggtattcct ctgataatca acttacaaat cgtgagtcag aaatattaca
      481 actcatagct gatggtaaaa caaacaaaga aatcgctaat ttcttgcagt taagcagaaa
      541 aacagtcgaa actcatagac tcaatatcat gaagaaatta gatgttcaca gtggtatcga
      601 gttgatcaaa acagctttac gtatgggtgt ttgcactata taaaaaaaag aggctttagg
      661 ctcattttgc tgaagcctta cgattgtcta gttcattaag cctggtatat acaatatata
      721 ttttggggca tattgctgtc tctttttttg gttatccatt gcaaaacccg cattataagt
      781 ccccaggcat tgccacgtta ctccacaacg tgaaaaatgg ttatataaaa tctcggttcc
      841 tattttaatg ttcagacaag ggttatctaa caaatctcgt tcatctttta ttatt
//
LOCUS       DAPHDX010000356          886 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPHDX010000356 DAPHDX010000000
VERSION     DAPHDX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.361"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>886
                     /locus_tag="RH058_004656"
     CDS             <1..>886
                     /locus_tag="RH058_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HDV3140077.1"
                     /translation="AEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIA
                     GPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHG
                     YVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKG
                     IHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEE
                     ALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKA
                     FAYALNQWPALTYYANDGW"
BASE COUNT          188 a          239 c          289 g          170 t
ORIGIN      
        1 gccgaacagc tggagttgat gcgtagcgcc ttccgggtta tccggacggt acgggaaaaa
       61 catgcctgta ctcagtgcga tgctatcgtg caggcacctg caccttcgcg gcccatcgag
      121 cggggtatcg ccgggccggg gctgctggcc cgcgtgctga cctcgaagta tgcagagcac
      181 accccgctgt atcgccagtc agaaatatac ggccggcaag gtgtggagct gagccgttca
      241 ctgctgtcgg gctgggtgga tgcatgctgc cggctgctgt ctccgctgga agaggcgctt
      301 catggctatg tcatgactga cggcaaactc catgccgatg ataccccggt ccaggtactg
      361 ctgccgggta ataagaagac gaagaccggg cggttgtggg cgtatgttcg tgatgaccgc
      421 aatgccgggt cagcgttggc acctgcagtg tggttcgctt acagcccgga cagaaaaggc
      481 atccatccgc agactcatct tgcttgcttc agcggggtgc tgcaagcgga tgcgtacgcc
      541 gggttcaacg agctgtatcg caatggtggg ataacggaag ctgcctgctg ggctcatgcc
      601 cgccgaaaga tccacgatgt gcacgtccgc atcccgtcag cactgacgga agaagccctg
      661 gagcagatcg gtcagttgta cgccatagag gcggatataa ggggaatgcc ggcagagcag
      721 cggcttgctg aacgtcagcg aaaaacgaaa ccgctgttga aatccctgga aagctggttg
      781 cgtgaaaaga tgaagaccct gtcgcgacac tcagagttgg cgaaggcgtt cgcgtacgca
      841 cttaaccagt ggccggcact gacgtactat gcgaacgatg gctggg
//
LOCUS       DAPHDX010000357          869 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPHDX010000357 DAPHDX010000000
VERSION     DAPHDX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.362"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(86..538)
                     /locus_tag="RH058_004657"
     CDS             complement(86..538)
                     /locus_tag="RH058_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140078.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQQLLKQLRKVDSGK"
     gene            complement(568..>869)
                     /locus_tag="RH058_004658"
     CDS             complement(568..>869)
                     /locus_tag="RH058_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV3140079.1"
                     /translation="TVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCR
                     IRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          213 a          258 c          133 g          265 t
ORIGIN      
        1 taaatactcg accactctga ctttcaaaat agtgaatgga cacttttcga cctgacgcat
       61 caaatccatc tttataaact ttactctatt ttcctgaatc tactttacgt aattgtttca
      121 ataactgctg atcctttaca acattccttg tagtgccatt ttggacattc ctaacccctc
      181 tgggatactg aggagcatcc ttaatagacc tgtaggtatt tcttatttcg ccatcctcat
      241 ctggctccca ccctcccgaa cctcctgtac tgccactgcc caaacagttc ttctcttcat
      301 ccgtcaaatc cttcccgaca tttggattct cttttgatga atcatcattt ccaaacacgc
      361 tggaaaaagc accataggga tctcgccaga attccatttg actttgtgca tttaataatt
      421 cggcttcacc ccgcatctcc atacttttct caatcgcatc cccaacagcc tcgcgattgt
      481 tgtatattac tataccacca ccaataacca ccgctccaac tgccgcaccg actaacatcc
      541 ctggagggcc tcctacccag ggcaagatta cgaaagcccg ctccccgcaa ggactgacgc
      601 cagatagttc ctgtccatgg ctgcttttcg catcttacgt cttaaccctg ccttgaatac
      661 cttatcattc gtcaaaatat taatagcaat gtgccgtatc cctgaaaata attccgctgc
      721 atttcctctt cttattctgc agtcgtcttc attcattacc acgtccagac gccagtgcag
      781 cttattctcc acgtgccagt ggtttcggat tgctgtggcg aacttctctg cggttaaatc
      841 agcagaactg atataatatc tgaccgtca
//
LOCUS       DAPHDX010000358          859 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPHDX010000358 DAPHDX010000000
VERSION     DAPHDX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.363"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>859
                     /locus_tag="RH058_004659"
     CDS             <1..>859
                     /locus_tag="RH058_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3140080.1"
                     /translation="LRDYSEKKTRLKKILPYTVRKRNVVNRATKHFRLTNIANYLSWL
                     SQILKQQQGSINEFVDKIRERRPKLRRRSRQDQPEKSLDGNQINHLLEVIKCGAAFNP
                     FGENLQIRNRLIILVLYSLGIRSGELLNIRISDINFSDSSLAIRRRADDKNDPRVKQP
                     LVKTMERKLPLSSALAKELHNYITSVRRKFKNARKHEYLFVTHKSGPTQGMPLSSMAY
                     HKVIDSIRNVMPELCNLTGHKLRHTWNYEFSKKLDEMPERVSEAEQEKMRSNLMGWTP
                     GSGTAQIYN"
BASE COUNT          303 a          156 c          177 g          223 t
ORIGIN      
        1 ctttaagaga ttatagtgag aagaagaccc ggttaaaaaa aattcttcca tatactgtca
       61 ggaagaggaa tgttgtaaac cgtgcaacca aacacttcag attaaccaat atagcaaact
      121 atttgagttg gttaagtcag atacttaaac agcaacaagg aagtataaat gaattcgtcg
      181 ataagattag agagagaaga ccaaaactaa gaaggcgcag ccgtcaagac caaccagaaa
      241 aatctcttga tggaaaccaa attaatcatt tattagaagt cataaaatgt ggagcggcat
      301 ttaatccatt cggtgaaaat cttcagataa gaaaccgact tattattctg gtgctttatt
      361 ctttgggaat aaggtcggga gagcttctta atattcgtat atcggatatt aactttagtg
      421 attccagtct ggctatccgt cgtcgggctg atgataagaa cgatccacga gttaaacaac
      481 cattggttaa gacgatggaa agaaaactac ctctatcttc ggcattggca aaagaattgc
      541 acaattacat taccagtgtc cgtaggaagt ttaagaatgc cagaaagcat gagtaccttt
      601 tcgtaaccca taagtctggc ccaacacaag gtatgccttt atcgagcatg gcttaccata
      661 aagttatcga tagcatcaga aatgtcatgc ctgagctttg caatcttacg gggcacaaac
      721 tcagacatac ctggaactat gaattctcta aaaaattaga tgagatgcct gagcgagtaa
      781 gtgaggctga acaagaaaaa atgcgttcta atcttatggg atggactccc ggttctggaa
      841 ctgcacagat atataacaa
//
LOCUS       DAPHDX010000359          850 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPHDX010000359 DAPHDX010000000
VERSION     DAPHDX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.364"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..>850
                     /locus_tag="RH058_004660"
     CDS             42..>850
                     /locus_tag="RH058_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3140081.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHP"
BASE COUNT          272 a          182 c          155 g          241 t
ORIGIN      
        1 agtgaatata ccatggaaga aaaatataac cggagtagtg tatggccaat ctctacgact
       61 tgaaaaagtt cgacttaaac cttcttgtca tatttgagtg tatttaccag catttaagca
      121 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
      181 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
      241 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
      301 caattaatat agtcaataag tccgaactaa agaaaaactt tattatttac ggccctcagc
      361 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
      421 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
      481 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
      541 cattgcatac gattcgcaat actctaattt gtagtaatag gcatccacga attactgaca
      601 atagcactta tgaacaaatt atggcagaag agttcaccca attaatatct aaatcagcag
      661 gggttgatga tattcaaatg gaaatagacg agagatttat gaatcgcaaa atctcatttc
      721 gtggttcctc tttgctgaca atcattaata gcattgcagt tacagattta ctgggaatag
      781 tcccctatga actttataat tcctaccgtg actttcttaa tttaaaagag ataaaactag
      841 aacatccgtt
//
LOCUS       DAPHDX010000360          847 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPHDX010000360 DAPHDX010000000
VERSION     DAPHDX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.365"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>847
                     /gene="espL4"
                     /locus_tag="RH058_004661"
     CDS             <1..>847
                     /gene="espL4"
                     /locus_tag="RH058_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDV3140082.1"
                     /translation="QTIAPIFEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLN
                     GQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTL
                     ALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTARLRIKNTT
                     EGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSV
                     AIIPALPKEQLLMLKGSVDEIPPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRT
                     EMLTA"
BASE COUNT          294 a          166 c          156 g          231 t
ORIGIN      
        1 ctcaaaccat tgccccaata tttgaagaat acctaatgga aattttacct caacgctcag
       61 acactcttga taaacaagaa ttaaacctaa aatcagatag aaaagaaaaa gaattcccaa
      121 gaattaaact taatggtcaa tgttattttc cggggcgacc ccaaaaccgt atagtatgcc
      181 gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac aaaccccatc
      241 aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc
      301 agactttggc gttgatttcc agccgtcctg aggggcgttg tgttgctgcc tgtggtgatt
      361 tcgggctagt tatgaaggca tatttcgaca agatggaatc aaatggcatc agtgttatgg
      421 cagccatatt attggtggat aaccatgctt tgacggcgcg gctaagaata aagaacacaa
      481 ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact aacgataaaa
      541 taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg gattttatga
      601 atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa
      661 taattccagc acttccgaaa gaacagctat tgatgttaaa aggatctgtg gatgaaatac
      721 cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag aatcaccaac
      781 ttacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca gaaatgttga
      841 ctgccta
//
LOCUS       DAPHDX010000361          842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPHDX010000361 DAPHDX010000000
VERSION     DAPHDX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.366"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>842)
                     /locus_tag="RH058_004662"
     CDS             complement(<1..>842)
                     /locus_tag="RH058_004662"
                     /inference="COORDINATES: protein
                     motif:HMM:NF027494.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3140083.1"
                     /translation="GGSAVDTTIEEGLQTVLNGGNVSGTHISGGEQRVSSGGSAVDTT
                     IEEGFQTVLNGGNVSGTHISGGEQNISSGGSAVDTTIEVGLQTVFDGGSVNGTIIDGG
                     EQHISSGGSAINTTLDGYQTVFNGGNATGTTINGGFQNISSGGSATSTIINAGFQEVS
                     SGGSATSTTINAGFQALYDSSIASGTVINNGFQLISSGGSAINTTIKGGFQEVSDGGR
                     AIETTITSGWQNVLSGGVATETLIVGGVQTIYDGGSASEITINSGYQVISSGGSVTTT
                     TIYRG"
BASE COUNT          194 a          251 c          190 g          207 t
ORIGIN      
        1 ccacggtaaa tggtcgtggt ggttacactg ccaccgcttg agatgacctg gtaaccactg
       61 ttgattgtaa tctccgacgc tgagcctccg tcgtaaatcg tctggactcc cccgacaatg
      121 agcgtctcag ttgcaacccc accacttaat acattctgcc agccactagt aattgtggtc
      181 tcaatagctc tgccaccatc gctaacctcc tggaatcccc ctttaatggt ggtgttgatg
      241 gcagagccac cactggaaat gagctggaag ccattattaa tgacggtgcc gctggctata
      301 ctgctatcgt agagagcctg gaacccggca ttaatagtgg tgctggtagc actgccacca
      361 ctggaaacct cctggaatcc ggcattaata atggtgctgg tagcactgcc accactggaa
      421 atattctgga acccgccatt aattgtggtg cctgttgcgt tgccaccatt gaaaactgtc
      481 tgatagccat ccagtgtggt gttaatcgca cttcccccac tggaaatgtg ctgctcccca
      541 ccatcaatga tcgtgccatt aacacttccg ccatcaaaaa cagtctgtag tcccacctca
      601 atggtggtgt ctacagcact accaccgctg gaaatgttct gctcacctcc gctaatgtga
      661 gtcccggaga catttccgcc attgagaaca gtctgaaacc cctcctcaat ggtggtgtct
      721 acagcactac caccgctgga aactcgctgc tctcctccgc taatgtgagt cccggagaca
      781 tttccgccat tgagaacagt ctgaagcccc tcctcaatgg tggtgtcaac ggcactgccg
      841 cc
//
LOCUS       DAPHDX010000362          837 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPHDX010000362 DAPHDX010000000
VERSION     DAPHDX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.367"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          218 a          160 c          180 g          279 t
ORIGIN      
        1 tgaaagacag agagattatt gagtaatctt atttcataga aaaatattaa tgacgtcctg
       61 tggcgtatgc atgatgcaag gttgaacgca tgtaatatga tatttatcac gtaaatgcag
      121 tgtaatcttc ttgcattgtt tttaggttgc tattccgttt atctgaggac tgctggctat
      181 tgttgtgcag ttttcctgat acagctttat gcaggttttc cagcattaca gtaaaacccg
      241 ggaacatcag tgctgctcgt ttctttctcc tgtatccact cccgcttata gatacttcct
      301 taaatattct ctcgtctgac taaagcatat atacccgttc atgggatgtt ggctgtaatg
      361 attttcctga gcggattatt actgatgacc ataagtatgt ctcagcacag tcatcgtaac
      421 gtgctggtta acctggccat ggtagccaaa tcatcatcct gtatattctg ttgagggagg
      481 cactggtgat tgttcagaat acggctgatg cgaaaaagtg caggctgttt cctttattgg
      541 tgtgatgtac agcctcttta cgctctactt ttccggggaa gaagccatgc tgtagcactt
      601 tattgttact tttcctggct ggacactgta tggtctggta tccccccacg ttttgaacgg
      661 aaaaaacaga tttaaagttg ccagtagtct gtaagtttct gcatcccatt tccccacaga
      721 cagtattctc ctgcaatgtg atgaatggtt tcctggaaca ggaaaggaac taagtcagat
      781 agtggagaaa gtggttaaca cgttgttttt aattaaaatt ttggtaataa caataac
//
LOCUS       DAPHDX010000363          835 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPHDX010000363 DAPHDX010000000
VERSION     DAPHDX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.368"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..835
                     /locus_tag="RH058_004663"
                     /pseudo
     CDS             1..835
                     /locus_tag="RH058_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          273 a          125 c          171 g          266 t
ORIGIN      
        1 ttacttaagc caagtagtac gcgtagtgtt attttagaaa cgccgccaaa tgtctgtatg
       61 gaatcacgta attcatatga aaaaaaatag atgagatttc atctttgtca gagtcaaagg
      121 aacaccccat agatattcaa gaaaaaaaag atgcgtttgt gaatgagttc aaggggatat
      181 tatttgataa aaatggaagg tcttcagagt ttctacttaa tttttatgaa tgttgctatg
      241 agtttttacc aagagcgcag cctcaggata aaatcgaaag ctataattca gcactgcaag
      301 ctttctccat cttttgttca tctacgttga tacataataa tataggcttt gatttcaaat
      361 tatttccaga agtcaaactg tgtggggaaa atcttgaaac ggtattcaaa tataaaaatg
      421 gcgatgatgt ccgggagata gccaaaatta acattgctct ccaaaaagaa gaggatggtt
      481 tatataattt aggtggattg gattttaagg gatgcttctt ttctggacag aacttcagta
      541 actatgatat tcaatatgtg aactggggaa cgtcattgtt tgatcttgat actccgtgta
      601 tttttaatgc gcctgcttac aacaagagta atgaaaaatc attaaaacct gtgagcgaaa
      661 acggtttaag tggagtcttg actgatcgta ataataaaat aaaactcatc acgggcgtgg
      721 caccattcga tgatatttta tttatggatg atgactttga tgatagttcc tctgaggatg
      781 atcccgttga gaatagtcct gttgtgacta gtcccgttgt atcaagttct aaaag
//
LOCUS       DAPHDX010000364          834 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPHDX010000364 DAPHDX010000000
VERSION     DAPHDX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.369"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..687
                     /locus_tag="RH058_004664"
     CDS             <1..687
                     /locus_tag="RH058_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDV3140084.1"
                     /translation="LGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            complement(703..822)
                     /locus_tag="RH058_004665"
                     /pseudo
     CDS             complement(703..822)
                     /locus_tag="RH058_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          256 a          195 c          177 g          206 t
ORIGIN      
        1 ctgggttggt actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg
       61 gaaaactgga aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta
      121 ggctataaga ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct
      181 cgctctaaag gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct
      241 aaaatctact cagcgtcggc aaaggagcca tggattctag caactaactt acctgttgaa
      301 attcgaacac ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc
      361 ttccgagact tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc
      421 tcagagcgtt ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt
      481 gcgggcgttc atgctcagaa acaaggttgg gacaagcact tccaggctaa cacagtcaga
      541 aatcgaaacg tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac
      601 acaataacaa gggaagactc actcgtggct gcaaccctgc ttactcaaaa tctattcaca
      661 catggttacg ttttggggaa attatgaggg gatctctcag tgctcagcat gtttgagtgc
      721 tttactcgcc gcgggttgag aaatatgtaa aacttcagcc gcacgcgtta aggaaccaca
      781 agtcattaca gcataaaaga tatcgagatg tcttaatttc attccatgta gcct
//
LOCUS       DAPHDX010000365          829 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPHDX010000365 DAPHDX010000000
VERSION     DAPHDX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.370"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..710
                     /gene="ydeO"
                     /locus_tag="RH058_004666"
     CDS             <1..710
                     /gene="ydeO"
                     /locus_tag="RH058_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV3140085.1"
                     /translation="LDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFTENTIKKYL
                     QCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAA
                     CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTF
                     SQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRC
                     QSHTGMNTGNTMSALAI"
BASE COUNT          253 a          164 c          168 g          244 t
ORIGIN      
        1 ttttagataa agattacgcc ttctctgacg gcgagatcct gatggtagat aacgctgttc
       61 gtacgcattt tgaaccttat gagcggcatt ttaaagagat cggatttact gaaaatacca
      121 ttaaaaaata tctacaatgc actaacatcc agacagtgac ggtgcctgtt cctgcgaagt
      181 ttttacgtgc ttcaaatgta ccgactggat tgcttaatga aatgattgct tatctcaact
      241 cggaagaacg caatcatcat aatttttcag aacttttgct tttttcttgc ctgtctattt
      301 ttgccgcatg caaaggtttc attacactat taactaacgg tgtgctatcc gtttccggaa
      361 aagtgagaaa tattgtcaac atgaagctgg cgcacccatg gaagctgaaa gatatttgtg
      421 actgcctgta catcagtgaa agcctgttga agaaaaaact aaagcaagag caaacgacat
      481 tctcacagat tctattagat gccagaatgc agcacgcaaa aaatttgata cgcgtagaag
      541 gttcagtcaa taaaattgcc gaacaatgtg gttatgccag tacatcttat tttatttatg
      601 cgttccgcaa acatttcggc aacagtccga agagagtttc taaggagtac cgttgtcaaa
      661 gtcacacggg tatgaatacg ggcaacacga tgagtgcttt agctatttga ttatttgcta
      721 acgagtagtc aaccacgcac gctacgtaag aattatatgg ggcagccatt ccctgccccg
      781 cgttgttttt aggcgatata tttattgaaa taaataagtg acatccatc
//
LOCUS       DAPHDX010000366          827 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPHDX010000366 DAPHDX010000000
VERSION     DAPHDX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.371"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..>827
                     /locus_tag="RH058_004667"
     CDS             56..>827
                     /locus_tag="RH058_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3140086.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTQSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVAD"
BASE COUNT          274 a          161 c          161 g          231 t
ORIGIN      
        1 tatacttagc aaactatgtg atctccattt cgattgattt agtgtttatt gacgtatgta
       61 ctggattatt aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca
      121 taacgccgat cttaacgtcg ttctgacaac gccagccagt cgatgtttat cacttctact
      181 tgaagctttt cctgatgtgg ttgcacaaca agattttttc accagagtct gggaagaaga
      241 aggtatgcgt gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt
      301 tcgcgctgtt ggtgatacta cccagtcgct aattgcaacc gtgccgagaa gaggattcaa
      361 gatccataat gacattaaca tacaaaatca tgtaataaac tcgtcaacag acgcacatac
      421 acacaatgcc ccacctgcca taaaagttaa tgcgggatac aaagagagca ttggtggcgc
      481 aaagaatttc aataacaaaa tcctcaaaca tataaaaagt catctaatta tgttgagcgc
      541 atttgtcata ggcgcatatt ctgcatattg gctatggaat aataatcaac cgaagccatt
      601 tttcaaggat tacaagactg ttgcggaaat taatggctgc cattttaatg taacagaaga
      661 tacaattgac gggttgaaag agttcgataa atataaaaca cggatactgg attcggggat
      721 taactgtaaa aaacatccgt ggttatactt tcctcttgca aaatcctcac cggggatgat
      781 tgttatggca tgcaataaaa attataacca acatgaagtg gcagact
//
LOCUS       DAPHDX010000367          824 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPHDX010000367 DAPHDX010000000
VERSION     DAPHDX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.372"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..>824
                     /locus_tag="RH058_004668"
     CDS             171..>824
                     /locus_tag="RH058_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV3140087.1"
                     /translation="MLDTILIKTASRCNLDCTYCYVYRGADTSWQDQPYRMNDATIEK
                     VVERITEYSLLQETGFAIVLHGGEPLLLGERRLESLLSGLRRVLNPQKYPISIQTNGV
                     LITEKILNLCSKYRVSISVSIDGTRKANDIARFTRGGNSSFEDVINGIKKLSSHQDHA
                     FLFAGTLSVIQPNIPAEDTYHYLKSINSPSIDFLFQDGNYDRMPYGKKDLQTTEYGEW
                     "
BASE COUNT          289 a          128 c          181 g          226 t
ORIGIN      
        1 gtatgatgca aaaccaaacg gaagaagcaa aaagaatctt aaaaacagat ggttacagcg
       61 gatctaaccc ggtgttatct agactgatgg aagaagttag aaatgaaaaa aacacaacgg
      121 taggtagccg ttatgatcga gtccataatc gccataaccg ttaaatttct atgcttgata
      181 caattttaat caagactgcc agtaggtgca atttggattg cacctactgc tatgtatatc
      241 gcggggctga tacaagctgg caggatcaac cctataggat gaatgatgcc acgatagaaa
      301 aagtggtgga aaggattacg gaatatagtc ttttacagga aactggattt gccatagttt
      361 tacatggagg tgaacctctt cttttggggg aaagaagact tgagagttta ttgtcaggtt
      421 taaggagagt tttaaatcct caaaaatacc cgataagtat acaaaccaat ggtgttttga
      481 taactgagaa aattttgaac ttatgttcaa aatatagagt aagcatttcg gttagcattg
      541 acggcacaag aaaagctaat gatatcgcga ggtttacacg tggtggtaac agtagttttg
      601 aggatgtaat taacggaatt aaaaaattaa gttcacacca agaccacgcc ttcttgtttg
      661 cagggacatt atcagtaata caaccgaata ttcctgctga ggatacatat cattacctga
      721 agagtataaa ctctccaagt atagattttc tatttcagga tggaaattat gataggatgc
      781 cttatggaaa aaaagatctt caaactactg aatacggaga gtgg
//
LOCUS       DAPHDX010000368          807 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPHDX010000368 DAPHDX010000000
VERSION     DAPHDX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.373"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..765
                     /locus_tag="RH058_004669"
     CDS             49..765
                     /locus_tag="RH058_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3140088.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          267 a          139 c          171 g          230 t
ORIGIN      
        1 ttaacgattt accagtaatg taaataaatt ttcgaggaga tcattccagt gggacgtaaa
       61 tgggccaata ttgttgctaa aaaaacggct aaagacggtg caacgtctaa aatttatgca
      121 aaattcggtg tagaaatcta tgctgctgct aaacaaggtg aacccgatcc agaattaaac
      181 acatctttaa aattcgttat tgaacgtgca aagcaggcac aagttccaaa gcacgttatt
      241 gataaagcaa ttgataaagc caaaggcggc ggagatgaaa cgttcgtgca gggacgttat
      301 gaaggctttg gtcctaatgg ctcaatgatt atcgccgaaa cattgacttc aaatgttaac
      361 cgtacgattg ctaacgttcg cacaattttc aataaaaaag gcggcaatat cggagcggca
      421 ggttctgtca gctatatgtt tgacaatacg ggtgtgattg tatttaaagg gacagaccct
      481 gaccatattt ttgaaatttt acttgaagct gaagttgatg ttcgtgatgt gactgaagaa
      541 gaaggtaaca ttgttattta tactgaacct actgaccttc ataaaggaat cgcggctcta
      601 aaagcagctg gaatcactga gttctcaaca acagaattag aaatgattgc tcaatctgaa
      661 gttgagcttt ccccagaaga tttagaaatc tttgaagggc ttgttgatgc ccttgaagat
      721 gacgacgatg tacaaaaagt ttatcataac gtcgcaaatc tctaattatc ttttaaagaa
      781 atctgtcttt acggcagatt tctttaa
//
LOCUS       DAPHDX010000369          797 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPHDX010000369 DAPHDX010000000
VERSION     DAPHDX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.374"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(15..686)
                     /gene="queE"
                     /locus_tag="RH058_004670"
     CDS             complement(15..686)
                     /gene="queE"
                     /locus_tag="RH058_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV3140089.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          199 a          209 c          177 g          212 t
ORIGIN      
        1 ttataaatgt ttaatcaggc aatatttaga tatttatgtg tttgcatcga caaacgccaa
       61 ttacgcgcaa tgcaggtttc aatgcacaaa cgtgtggcat catccttttg gctaatcggc
      121 tgcagtgcaa tgactcgcgg tttatcatcg gtcagcgttg ccagtagttc atccagtgct
      181 tcaatatcac gtacgcgccc cactggatgc ttgatttcgt tggctcgctc cagtgcctgt
      241 gacaacacgt catagccgcc gcgcatgttc agctttggcg atacggtaac ccaggtattc
      301 ggtgtgcagc gtacttcatg agttccactg gtttcgatct ggcagctaaa accgttcttt
      361 tcgagcaggt cagtcagtgg cagcaaatca tgaatgcaag gctcaccacc cgtaatcacc
      421 acatgccgcg cggtatatcc ctgccgacca atgacagcca gcaaatcttc actgctcgcc
      481 gccccccact tatcactctc tttggtcttc gccagaatgc tgaaaaggga gacttcccga
      541 tcctcaagct tttcccaggt gtgtttggtg tcgcaccagg cacagccaac cgggcatccc
      601 tgtaaacgaa taaaaatggc ggggacgccg gtaaagtaac cctcaccttg cagggtctgg
      661 aacatctcgt taatcgggta ctgcatagca ttctctgtga agtggataat tgttaattat
      721 tgcagatcct gccacaacaa tcatgtctta ttaacattct gttacaggca gatttaaaga
      781 aaaatgcgaa acatatc
//
LOCUS       DAPHDX010000370          795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPHDX010000370 DAPHDX010000000
VERSION     DAPHDX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.375"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(135..>795)
                     /locus_tag="RH058_004671"
     CDS             complement(135..>795)
                     /locus_tag="RH058_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDV3140090.1"
                     /translation="EENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYL
                     CVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKG
                     KFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGV
                     M"
BASE COUNT          228 a          193 c          143 g          231 t
ORIGIN      
        1 ataagacatg agactaatta actcgctcac atcacgagtg gagtctaata gtgaaagtct
       61 atgcatggaa aagaatagta ttaaaagcga taaagccagc catatagctc tgaataaaaa
      121 aataatatat ttcattacat cactccggtg aatacaaatc taataaaacg tccaatgtta
      181 tttccggagc acttcatctt acatgtttct aaagaatcat tagaccagtc ttggcagttt
      241 ttaaatagcc ctaaatttat aggtgtgaat atatcaggca atacagaata acgcggcctt
      301 acctctttaa acttcccttt taaacagttc tccacacaac tattatcggt ttcaacgagg
      361 tcacaacggc cagcagcttc ttttgtatca ttacttgctt tacctggccc ccatagacca
      421 tctccttttg attctccccg ttggtcttgg ccaccacaag tatagtcccc tttaccatct
      481 gggacacaaa gatattgatg atagaacgga ttcccccata tatctggacc gcttctttca
      541 ccagttccgc ccattgaatg cagtggctta atgcatttta gtgtttctaa tcctaatgga
      601 tctatatttg agatcggatt caacggatac tgataaaaat tccatccccc cttcagccca
      661 atcggatcct gagtgatata tcgcccctgc agcgggtcat aatagcggtg gcggttgtaa
      721 tacaggccgg actcctcatc atactgctgc cccggcagcc ggataagctg ctgcagctga
      781 tgcgggttct cttca
//
LOCUS       DAPHDX010000371          793 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPHDX010000371 DAPHDX010000000
VERSION     DAPHDX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.376"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..698)
                     /locus_tag="RH058_004672"
     CDS             complement(<1..698)
                     /locus_tag="RH058_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3140091.1"
                     /translation="MKKVFAVSVVGAACVFAVNAGAEEGKSGFYLTGKAGASVVSLSH
                     QRFLSGNEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGNADTKY
                     NLYKDDWSRGDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTGISTW
                     DYGYGYSGRESLSRSGSADNFAWSLGAGIRYDVTPDIALDLSYRYLDAGDSSVSYKDE
                     WGDKYKSEVDVKSH"
BASE COUNT          214 a          219 c          147 g          213 t
ORIGIN      
        1 tcatgacttt taacatcaac ctctgattta tatttatcgc cccactcgtc cttgtaactc
       61 acactgctgt cacccgcatc aagatagcga tagctgaggt ccagagcgat atccggagta
      121 acgtcatagc ggatacccgc gccaaggctc catgcgaagt tgtcagcaga gcctgaacgt
      181 gacaacgatt cacgaccact gtatccgtac ccgtaatccc aggtactgat accggttgtt
      241 ttctggtgaa ttctggcgta gccaatccct gcagataccc atggtgtgaa tgcgctgtca
      301 ttccggaagt catagtacgc attcagcatc agagtgttga ctgacacctc attcttcagg
      361 tcaccacgcg accagtcgtc tttataaaga ttatatttcg tgtcagcatt tccacgagca
      421 taaaactcca gttctgtacg aaccggaata ctgaactgtg gataaaaatc ataaccggct
      481 gcgataccgc cactgaatac cgtgtcatgg tcatccccac ctttatactt tgacgtttct
      541 tcttcatttc ctgacaggaa acgctggtgt gaaagtgaca ccacagaggc accggcttta
      601 ccggtcagat aaaaaccgct tttaccttcc tcagcacctg catttactgc aaatacacag
      661 gctgcgccca ccactgaaac agcaaaaacc tttttcattt caattatctc cgttattgta
      721 tcatcacttt aatatatatt tttaatattt aaaaaccgga ctaaaggcag cctcccccct
      781 ccacactacg gaa
//
LOCUS       DAPHDX010000372          788 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPHDX010000372 DAPHDX010000000
VERSION     DAPHDX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.377"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..88
                     /locus_tag="RH058_004673"
     CDS             <1..88
                     /locus_tag="RH058_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3140092.1"
                     /translation="KREYKEQEGNPEIKSKRRERIRKFFLSN"
     gene            85..477
                     /locus_tag="RH058_004674"
     CDS             85..477
                     /locus_tag="RH058_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3140093.1"
                     /translation="MKSDVSNSRLMISNPTHIAIGIYFKPHLSPIPLISVRVTNEVAL
                     GVRKYAKEIGIPIITDKKLARKNYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPV
                     QDEQLSSEDKFIEGEDTKIENKDDNLKN"
     gene            complement(538..>788)
                     /locus_tag="RH058_004675"
     CDS             complement(538..>788)
                     /locus_tag="RH058_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDV3140094.1"
                     /translation="LISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEHPEYISS
                     AELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
BASE COUNT          266 a          137 c          153 g          232 t
ORIGIN      
        1 taaaagagaa tacaaggagc aagaaggaaa tccagaaatt aagtctaaac gcagagagcg
       61 catcaggaaa ttctttctga gcaattgaag tctgatgtca gtaatagtcg tttgatgatt
      121 tccaacccta ctcacattgc aatagggata tactttaagc cacatctttc acctattcca
      181 ttgatttctg taagagtaac taatgaggtg gccttaggcg taaggaaata tgcaaaggaa
      241 atcggaatac caattatcac agataaaaaa ttagcacgaa aaaattatgc tacccatcgt
      301 cgctacgatt atgttagctt cgaaaatata gatgaaatat tacgtcttct gctgtggctt
      361 gaagatgtgg agaatgctgg gcaacctgtt caagatgaac agctctcttc agaagataaa
      421 ttcattgagg gtgaagacac aaaaatcgag aataaagacg ataatttaaa aaattaaaca
      481 tctgtcgtag tcagaaattt ataagaatct acacgcaaaa agcgtgtaga tccttattta
      541 atgagttaaa cgatcttgtg aaatgataag caattgatac atactcatct ctttataagc
      601 tggaactgta gtaacctgtg caaattcgcg atatagcttt tgctccagtt ctgctgatga
      661 tatatattcc gggtgttcgc taaagaactg tcttgcatct actatcgctt gggattcgcc
      721 acttctttta ttttgattct cctttaatga attttctctg gtgcatgcag agagcaacga
      781 tatcagcg
//
LOCUS       DAPHDX010000373          778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPHDX010000373 DAPHDX010000000
VERSION     DAPHDX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.378"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..778)
                     /locus_tag="RH058_004676"
                     /pseudo
     CDS             complement(1..778)
                     /locus_tag="RH058_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          223 a          178 c          128 g          249 t
ORIGIN      
        1 gatataatat ctgaacgtca tttctggctc tttcttttgt tctgctatta ttgaccgaaa
       61 ggagactgcc acgcagaatt ttttcagccc tttccattca aacgttaaat caataagttc
      121 atcagggaca tcgcaaacaa tataaagacg gatttcttct ctgccgtgac tcttttcact
      181 cattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt tttcctgaaa
      241 ggctttatta agcccccctg gtttccgtgt acagcgaata aataatcacc tccctgtttt
      301 tgtatcttct cagcaatatc tttcaggcaa cccatcgcat cagttgtgat aatttttcct
      361 ttaatatcca acgtgttaat aagctcaggg atagctgtaa tctcattgga tttctcatct
      421 gtcctgaacc agactgtgca ttgttaagaa cgcactaatg acatgaatca ctcccttgcg
      481 acgactcttg tcataagatt gccggagtgt tttccatcaa ttgcgatgac gtctttatca
      541 tttgaagaat ggcatccacg catccagtta ataaggcact cgtaaaattt tgcaggactg
      601 atacaggata taactctggc aatggtaccg tgaacaggaa taccattttc aaaatcacca
      661 tattgcttca aaaaatcgag atgagtttcc caaaaaccct atatcttccc agccttctgc
      721 accagaaata acggcacaaa tagtcaacag tagaatatcc gataatttat gttccact
//
LOCUS       DAPHDX010000374          763 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPHDX010000374 DAPHDX010000000
VERSION     DAPHDX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.379"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(592..763)
                     /locus_tag="RH058_004677"
                     /pseudo
     CDS             complement(592..763)
                     /locus_tag="RH058_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          229 a          157 c          160 g          217 t
ORIGIN      
        1 acccattcag ttattaagct ctcaggtata agtgacttca aaatacatag ctacattatt
       61 aaaacaagtg aatatccgga ggagtttttt tccaatgcag tatggggtaa aaaaaatata
      121 aaaccggggg aatattactc tgtgcaggct gtttctatgt ttacgacaaa ccagtttatt
      181 ctcctttgtc ccaaggatat tataaggttt tatcgggaaa gctggaagtt cgacctgctt
      241 aatgtagact tcgatacaaa taccaggaag aaactccagg aagaagcagc atattgcgta
      301 ccaatttctg caatatctgt aaaacgatgg gatatgcctc ttcagggctc gaaaccctca
      361 aattagaagg ccgctcgtgc gactgtatta gatagtaatg cggtatggaa aaatagtgat
      421 ggcaagatga acttgacttg gctaatcaaa taccggcgta gtgattagga aacagggctc
      481 ggataacatt attgcgcggt tcattgaacc tactgatgac gccggatata tagtcgtaaa
      541 ccgtttcgaa ccagtactta tatcaccagg agagtccata tatgaccacc gtttacagta
      601 aaaacggtat ctgttttctg atccctccag cgtcaaacta ccgttgtaat ggtgaggtct
      661 ggtgctattg taataattca gaatataatc tctgatttat ttccgaacct cgcccttgcc
      721 tgcgtaacca tccgtcgccc ccatcctgtt ttcagactgc gga
//
LOCUS       DAPHDX010000375          746 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPHDX010000375 DAPHDX010000000
VERSION     DAPHDX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.380"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(189..731)
                     /gene="fimA"
                     /locus_tag="RH058_004678"
     CDS             complement(189..731)
                     /gene="fimA"
                     /locus_tag="RH058_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3140095.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          230 a          153 c          152 g          211 t
ORIGIN      
        1 aaaataagaa atataaagaa atgattgctt tggtttctta caacacatca ttcccttttc
       61 atttaataaa tcggcatgat acaaagtgaa catgatttcg atttacattg ccaccatacc
      121 gcttcagaca accggtacat tactttgtta taagacgtga tatataattc aaaacaacgt
      181 ggttttgatt attcgtatct cataataaaa gtcgcgtcag catttgcttg ccctgctgat
      241 gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa gaacgttggt cccggggatc
      301 aagttctggt tggttgagaa gctattccca tcaggtttga gaattgcgga tgtattatca
      361 agtatttcaa taccgacacc cgttgctgtt gttgtattgg tactggatgc aatggccaat
      421 aaattatcat tgattgcatc agcctgacca gaaaatgcaa cggcagcatt agcggcaaca
      481 acaggatcgc agtcgttcaa ctgaatggtg aatggaatta atgcagaggt attaccaaca
      541 gcattgaaaa tatcggtacg atattgaccg agtgtgacaa cctggtctgc cgagtcagta
      601 ttcactgaac aggcagcatt caccagttcg ccttcaaagt gaattgtacc gccatctaca
      661 acggcagcat aactacccgt agcggcgaat agtgcggcag ccagcgcaga cgaaataaat
      721 cttaatttca tatatattcc ttcaat
//
LOCUS       DAPHDX010000376          745 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPHDX010000376 DAPHDX010000000
VERSION     DAPHDX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.381"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..291
                     /locus_tag="RH058_004679"
     CDS             <1..291
                     /locus_tag="RH058_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV3140096.1"
                     /translation="LDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALV
                     RTDQPGFSAGTSSQLINSIDILRARRATGLMTRDNYRTVNNIALGKHPGAKQ"
     gene            288..>745
                     /locus_tag="RH058_004680"
     CDS             288..>745
                     /locus_tag="RH058_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140097.1"
                     /translation="MKLALKLEADSINVQALNMGRIVVDIDGVNLAELINVACDNGYS
                     LRVVDESDRTSADCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLFVTLIRRYGVSLIHLDAGAE"
BASE COUNT          179 a          200 c          193 g          173 t
ORIGIN      
        1 ctggaccagc attatggcct cacactgaat gacacaccgt tttccgatga acgtgtgatt
       61 gagcagcata ttgaggcagg tatttcactg tgtgatgcgg tgaactttct cgttgaaaaa
      121 tacgccctgg tacgtaccga ccagccggga ttcagcgcag gaacttcgtc gcagttaatc
      181 aacagcattg atattctccg ggcacgccgg gcaaccggcc tgatgacccg cgataactac
      241 agaacggtaa ataacattgc cctgggtaaa catccggggg cgaaacaatg aaactggccc
      301 tgaaactgga agccgacagc attaacgtgc aggcactgaa catggggcgc attgtcgttg
      361 acattgatgg tgttaatctc gctgaactga ttaacgtggc ctgcgacaat ggctactccc
      421 ttcgtgttgt tgatgaatct gaccggacct cagcagactg cacgccacca tttgctgccc
      481 tgaccggcat acgctgcagt accgcacata tcacggcaaa ggacaacgcc tggctgtact
      541 cgctgtcgca tcagacgaat gacaccggcg aatcagaatg gattcatttt acaggcagcg
      601 gatatctgtt acgtaccgat gcgtggtcat acccggttct gcggcttaag cgcctgggcc
      661 tgtcaaaaac gttccgccgt ctgtttgtca cactcatccg acgttatggc gtcagtctca
      721 ttcatctgga tgccggcgct gaatg
//
LOCUS       DAPHDX010000377          742 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPHDX010000377 DAPHDX010000000
VERSION     DAPHDX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.382"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>742
                     /locus_tag="RH058_004681"
     CDS             <1..>742
                     /locus_tag="RH058_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140098.1"
                     /translation="ASEVSVTNRFTKFHEIAHYENYEYLSSSEDISNRISLNLQAVGL
                     TKNSAEKLARLTLATFVSGQIIQFSGSLADIIADAIAIAIGAPRYHIWRVPVGIISDM
                     DAFDFIETIAESSRCLLLKGANLSAFEIYGAAIRDIVVQRQIHPTNYDHLALIATWKQ
                     GPATFPDGGMLAELGPVIDTDTLKMRGLSATLPQLKPGCLAKDKWTNIDGLHLDSVDD
                     YVDELRALLDEAGFDGGTLWKRMIHIFYT"
BASE COUNT          224 a          126 c          157 g          235 t
ORIGIN      
        1 gctagcgaag tgagtgtcac caatagattt acaaaattcc atgaaatagc gcactatgaa
       61 aattatgagt atctttcatc ctccgaagac atatctaata gaatttcttt aaatttacag
      121 gctgttggat tgacaaaaaa ttcagcagaa aaattggcta gattgacatt agctaccttc
      181 gtttctggac aaatcattca attcagtggc tctttggcag atattatcgc ggatgcaatt
      241 gccattgcta ttggtgcacc acgttatcac atatggagag ttccagttgg tattatttct
      301 gacatggatg cttttgattt tatagagact atagctgaat catctcgctg tctccttttg
      361 aaaggggcca atctttcagc atttgagatt tatggagcgg caattagaga tatagttgtt
      421 caacggcaaa tacatccaac aaattatgac catctggcat tgatagctac ctggaaacaa
      481 ggcccagcta cattccctga tggaggaatg ttggccgagt tgggacctgt tattgatact
      541 gatacattaa aaatgcgtgg tttatcagct actttacccc aattgaaacc aggttgtctt
      601 gccaaggata aatggacaaa tattgatgga ctacatcttg atagtgttga tgattatgta
      661 gatgaattaa gagcattact ggacgaagct ggatttgatg ggggaacttt gtggaagaga
      721 atgattcata ttttctatac tt
//
LOCUS       DAPHDX010000378          740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPHDX010000378 DAPHDX010000000
VERSION     DAPHDX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.383"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>740
                     /locus_tag="RH058_004682"
     CDS             <1..>740
                     /locus_tag="RH058_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3140099.1"
                     /translation="IVIVTGESVRRDYMSVYGYPVPTTPWLNTAPGLFIDGYTSAAAS
                     TVPSLSRTLIYDYEQNPDSGNNVVALAAKSGYSTWWISNQGKLGEHDTRISVIASDAE
                     HTVFLKKGSFASRKTDDMLLLQETERALADTSSPKVIFLHMIGSHPNPCDRLNSWPNH
                     YLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHH
                     DGHVQGGYSVPLIITASDITSHQSVSRK"
BASE COUNT          174 a          191 c          179 g          196 t
ORIGIN      
        1 attgttatcg ttactggtga gagtgtgcgc agggattata tgtcggtata tggttatccc
       61 gtgccaacca caccgtggct gaatacggct cccggtttat ttattgacgg ctatacatcg
      121 gcagcagcca gtaccgtacc ttccctgagc cggacactga tttatgacta tgagcagaac
      181 cctgattccg gcaacaacgt ggtggcgctg gcagcaaaat caggatacag cacatggtgg
      241 atatccaatc agggaaaact gggagagcat gacacacgca tctctgttat tgcttctgat
      301 gcggagcata ccgttttcct caagaaaggc agcttcgctt cccgtaaaac ggatgatatg
      361 ttgttgttac aggaaacaga acgtgcgctg gcggatacat cctctccgaa ggtgattttc
      421 ctgcacatga ttggctctca tccgaatccg tgtgaccgac ttaactcctg gccgaatcat
      481 tacctggagc agtatccccg aaagattgcc tgttacctcg ccagcatcag taaactggat
      541 aactttctcg gtcagcttga tggtatcctt cgccggcatt cccgtcattt cgccatgctt
      601 tacttttctg accatgggct gtcggtcagc gacagtgcta atcctgttca tcatgatggt
      661 catgtgcagg gaggctacag cgttcccctg attattaccg ccagtgacat cacgtctcat
      721 cagtccgtca gcagaaaaat
//
LOCUS       DAPHDX010000379          737 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPHDX010000379 DAPHDX010000000
VERSION     DAPHDX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.384"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            139..723
                     /locus_tag="RH058_004683"
     CDS             139..723
                     /locus_tag="RH058_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3140100.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          215 a          150 c          151 g          221 t
ORIGIN      
        1 taatgaacaa tggttgtaaa ttttggtaac aaatatcatt gctcattaat atctagcatc
       61 tgcttataac atttgtgtca tgaatagtca ttcttcaaac cagtagtctg actaatggaa
      121 ttttattcaa ggatttttat gtctaaaaaa ttaggttttg ccctgtctgg tcttatgctg
      181 gcaatggttg caggtactgc ttctgctgat atggatggtg gtcagttaaa tatcagtggt
      241 ctggttgttg ataacacctg tgaaacccgc gttgacggcg gtaataaaga tggtctgatc
      301 ctgctgcaaa ctgcaactgt tggtgaaatc gatgctggcg tgctgaatga caccgttggc
      361 gctaaagcca aacctttcag catcaccgtt gactgcagca aagcaaatcc gaatccaggt
      421 agtacagcta aaatgacctt cggttctgtt ttctttggta atagcaaagg aacgctgaac
      481 aacgacatgt ctattaacaa cccgtctgac ggcgtgaata tcgctctgca caatattgat
      541 ggttccacta tcaaacaggt tcaaattaac aaccctggcg atgtgtatac taaagccctg
      601 gatgcaacga caaaatctgc tgtttatgat tttaaagcgt cttacgttcg tgccgtcgca
      661 gaccaaacag caactgctgg ttatgtaaaa actaacactg catacaccat tacttatcag
      721 taagtattca ccaatta
//
LOCUS       DAPHDX010000380          720 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPHDX010000380 DAPHDX010000000
VERSION     DAPHDX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.385"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..323
                     /locus_tag="RH058_004684"
                     /pseudo
     CDS             42..323
                     /locus_tag="RH058_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            411..>720
                     /locus_tag="RH058_004685"
     CDS             411..>720
                     /locus_tag="RH058_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140101.1"
                     /translation="MKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKT
                     I"
BASE COUNT          230 a          113 c          194 g          183 t
ORIGIN      
        1 aagaaaaatg gatgtgtttt gaatttccta catagtaaat aatgctggcg ctgatggatg
       61 cggatggaaa cattgcgtgg agcggggagt atgatgagtg gggcaaccag ctgaatgaag
      121 agaacccgca tcacctgcac cagccctacc ggctgccggg gcagcagtat gataaggagt
      181 cggggctgta ctacaaccgg aaccggtact acgatccgtt gcaggggcgg tatatcactc
      241 aggacccgat agggctggag gggggatgga gtctgtatgc gtatccgctg aatccggtga
      301 atggtattga tccattaggg ttaagtcctg cagatgtagc gttaatgcga aaaaaagaac
      361 aactaaacca tcaaagagca tgggatatat tatctgatac ttatgatgat atgaagagat
      421 taaatttagg tgggactgat caatttttcc attgtatggc attttgtcga gtgtctaaat
      481 taaatgacgc tggtgttagc cgatcagcga aagggctggg ttatgaaaaa gagattagag
      541 attacgggtt aaatatgttc ggtatgtacg gcagaaaagt aaagttatcc cattctgaga
      601 tgattgaaga taataaaaaa gacttggctg taaatgaaca tgggttgaca tgtccattaa
      661 cacaagattg ctcaaataga tgtattgatt atattaatcc agagcataaa aaaacgataa
//
LOCUS       DAPHDX010000381          719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPHDX010000381 DAPHDX010000000
VERSION     DAPHDX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.386"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..252
                     /locus_tag="RH058_004686"
     CDS             <1..252
                     /locus_tag="RH058_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV3140102.1"
                     /translation="LAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYA
                     RLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNNL"
     gene            276..>719
                     /locus_tag="RH058_004687"
     CDS             276..>719
                     /locus_tag="RH058_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV3140103.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFNATNYDRLVKLSLNSNTLESINFPQ"
BASE COUNT          258 a          154 c          115 g          192 t
ORIGIN      
        1 ttagctgcga acgtagcaca cgctgccgaa gtctataaca aggatggtaa taaactcgac
       61 ctttatggca aggttaccgc tctacgttat tttactgatg ataagcgtga cgatggtgat
      121 aaaacttatg cccgtctcgg ctttaaagga gaaacgcaaa tcaatgatca aatgattggt
      181 tttggtcact gggaatatga ttttaaaggc tataacgatg aagccaacgg ctcgcgcggc
      241 aacaatctct gattgagtta agtgcccatc acaatatgat cactgacctt atattacaca
      301 atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat ttaaacgcca
      361 aaaacactac aaaactggaa tatttaaact taagcaataa caatttactg ccaaccaatg
      421 acattgatca actaatatca tcaaaacatc tttggcatgt attagttaac ggcatcaaca
      481 atgatccact tgcacaaatg cagtactgga ctgcagtaag aaatataatt gatgacacta
      541 atgaagtgac cattgattta tcaggactta atttaaccac tcaaccacca gggctgcaaa
      601 acttcacctc tatcaatctt gataataacc aactcacaca ttttaatgca accaactacg
      661 atagactcgt aaagctaagt ctgaatagta atactcttga gtcaataaat tttcctcaa
//
LOCUS       DAPHDX010000382          714 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPHDX010000382 DAPHDX010000000
VERSION     DAPHDX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.387"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>714
                     /locus_tag="RH058_004688"
     CDS             <1..>714
                     /locus_tag="RH058_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3140104.1"
                     /translation="MIDISENKYEGLRFENIFTRHRYIGPEGKALHLRSHQPRHLLNT
                     IGQRNGMSDLDLAKWSGRSLVTQNPVYNHVSEDEMLRKAETLDISTIGSQAFMIDSVK
                     PNLPVTPGMINLWEHGAVHATEFGYCVHDYMMSPCDKFRDCINCNEQVCVKGEEEKLQ
                     RLKERRNMTEGLLSRARESMTEEELGADRWVQHHELTLTRLNELIAILSNNEIPVGSR
                     IKPGGESFTQLGRVLNKFEN"
BASE COUNT          213 a          153 c          181 g          167 t
ORIGIN      
        1 atgatagata tttctgagaa taagtatgag gggctaaggt ttgaaaacat atttaccaga
       61 catcgctata ttggcccgga aggaaaagct ctgcatttga gaagccatca gcctcgtcat
      121 ttactcaata cgattggaca gcgaaacggg atgtcagatc tcgacctagc gaagtggtcc
      181 gggagatcgc tggttaccca aaacccggtt tacaaccacg tatcagaaga tgagatgctc
      241 cgtaaggccg agacactgga tatcagcact atcggctcac aggccttcat gattgattca
      301 gtaaagccga accttcctgt tactccgggt atgatcaatc tctgggaaca tggtgcagtg
      361 catgccaccg agttcggcta ctgtgttcat gactatatga tgagcccctg cgacaagttc
      421 agggattgta tcaactgcaa cgagcaagtt tgtgtgaaag gcgaagaaga gaagctccag
      481 cggctcaagg aaaggcgcaa tatgacagaa gggcttctct ccagagccag ggagtcaatg
      541 acggaagaag aactgggtgc tgaccgatgg gttcagcatc atgaactgac ccttactcga
      601 ttgaatgaat tgatcgcaat cctttcaaat aacgaaatac cagtcggttc acgtataaaa
      661 cccggtgggg aaagtttcac tcagcttggg cgagtattaa ataaatttga aaat
//
LOCUS       DAPHDX010000383          711 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPHDX010000383 DAPHDX010000000
VERSION     DAPHDX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.388"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>711
                     /locus_tag="RH058_004689"
     CDS             <1..>711
                     /locus_tag="RH058_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDV3140105.1"
                     /translation="GATTEEGAFVQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEV
                     PLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPE
                     SSGSYGFVRLEGDLMRTEVAGMSVTAGIYGAAGHSSVDVKDDDGSRAGTVRDDAGSLG
                     GYLNLIHNASGLWADIVAQGTRHSMKASSDNNDFRVRGWGWLGSLETGLPFSITDNLM
                     LEPQLQYTWQGLSLDDGK"
BASE COUNT          152 a          193 c          222 g          144 t
ORIGIN      
        1 acggtgccac cacggaggaa ggggcctttg tccaggggaa taagctgcag gccggtgcct
       61 ttaactactc cctcaaccgg gacagtgatg agagctggta tctgcgcagt gaaaatgctt
      121 atcgtgcaga agtccccctg tatgcctcca tgctgacaca ggcaatggac tatgaccgga
      181 ttgtggcagg ctcccgcagc catcagaccg gtgtaaatgg tgaaaacaac agcgtccgtc
      241 tcagcattca gggcggtcat ctcggtcacg ataacaatgg cggtattgcc cgtggggcca
      301 cgccggaaag cagcggcagc tatggcttcg tccgtctgga gggtgacctg atgagaacag
      361 aggttgccgg tatgtctgtg accgcaggga tatatggtgc tgcaggccat tcttccgttg
      421 atgttaagga tgatgacggc tcccgtgccg gcacggtccg ggatgatgcc ggcagcctgg
      481 gcggatacct gaatctgata cacaacgcct ccggcctgtg ggctgacatt gtggcccagg
      541 gaacccgcca cagcatgaaa gcctcatcgg acaataacga cttccgcgtc cggggctggg
      601 gctggctggg ttcgctggaa accggtctgc ccttcagtat cactgacaac ctgatgctgg
      661 agccacaact gcagtatacc tggcagggac tttccctgga tgacggtaag g
//
LOCUS       DAPHDX010000384          706 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPHDX010000384 DAPHDX010000000
VERSION     DAPHDX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.389"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(135..698)
                     /gene="fimA"
                     /locus_tag="RH058_004690"
     CDS             complement(135..698)
                     /gene="fimA"
                     /locus_tag="RH058_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3140106.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          209 a          178 c          141 g          178 t
ORIGIN      
        1 ttcatagcgc tccaaataca aaccatttct cattaaaaat gagatgaata ttggtaaatt
       61 gctcgggtgt gcgtagccat gccacacaca ccctgtttca atttcagatg acgtatagcc
      121 accttaagat tgaattatag atattgaact ttgaaggtcg catcagcgtt tgcagtacca
      181 gctacggact gcccgagagc aatataacga gcctggaatg gcagtatatt cgtgccatca
      241 atcaagtcgg ttttagcact aaaagtggcc ccatcaagta ttaatacctc acccgtacgg
      301 tccaaaattt gaataccgac attttgggca gagcctgccg ctgaactctg cagcgctaac
      361 gtatcgtcat tactggtgac agtagtaccc aggaatgcaa cagctgcatt actggaaaca
      421 ttggtatcac aatcattcag cttgatattg aagccaacgg cggagctgag gtcacccgct
      481 ttagccaggc gagaagaacg aacctgaccc agctcaaccg tttggttcat tgattctgaa
      541 tcaattgcac aagcggcatt taccacttca cctttaaaat gaataacacc gccattaaca
      601 gtggtcgtta ctgattcatc accctcggct gcgcttaccg cagcagacga catcgccaac
      661 acagaaacga caatcatacc aacatgtttg agtttcataa ttctcc
//
LOCUS       DAPHDX010000385          698 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPHDX010000385 DAPHDX010000000
VERSION     DAPHDX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.390"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..429)
                     /locus_tag="RH058_004691"
     CDS             complement(<1..429)
                     /locus_tag="RH058_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140107.1"
                     /translation="MIFYLIDKEVKDREMSFNTTHEKSEIYRLILRESELITAWVKSG
                     DTPSAVYGKLRDKNPDIIFSINGFLYNLRNFNYALYETATKNKSKTRLIILNHYDDIA
                     SAIRAGHTLKGVYKLVCPHITYNCFITQLRKTYPDLHSQGK"
BASE COUNT          205 a          121 c          131 g          241 t
ORIGIN      
        1 tttaccctga gaatgcaggt caggataggt tttcctgagt tgtgtaataa aacagttata
       61 tgttatatgt gggcatacga gtttgtaaac acctttaagc gtatgaccag cacggatagc
      121 tgatgcaata tcatcataat ggttgagtat tattaacctt gttttgcttt tgttctttgt
      181 ggctgtttca tatagtgcat agttaaagtt acgtaggtta tataaaaagc catttatcga
      241 aaagattata tccgggtttt tatcacgtag ttttccgtaa actgctgacg gcgtgtctcc
      301 agattttacc catgctgtaa tgagttcact ttctctaagg atcagacgat agatttcaga
      361 tttctcgtgt gtggtattaa aactcatctc tcggtcctta acttctttgt caatgagata
      421 aaaaatcata tttctataat ttagtcaaca ctctgtgcta catgcgatat ggatatacca
      481 gcaatatgca atgcgtgaga aaatgatgtt tttattgcat catctatgat gagtgaaaac
      541 aactaaagtt gaaagctaca caaaactggc cccatagtta tcgggactga ataccataaa
      601 ccattcatag tcaagcgtgt attcaaccat atcagttaaa ccggtttgac tggcctgaaa
      661 gtatctccat atccgttgtg ataattttgt tctcttta
//
LOCUS       DAPHDX010000386          674 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPHDX010000386 DAPHDX010000000
VERSION     DAPHDX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.391"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(33..305)
                     /locus_tag="RH058_004692"
     CDS             complement(33..305)
                     /locus_tag="RH058_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV3140108.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(348..584)
                     /locus_tag="RH058_004693"
     CDS             complement(348..584)
                     /locus_tag="RH058_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV3140109.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          192 a          140 c          116 g          226 t
ORIGIN      
        1 gcatggtaac ctctcatctt acttatgaaa ttttaatgta ttctgtttat tttcttacca
       61 ttgaacatac ttttattgtc agctgtcgct gttcttcggt ttggccctcc tgaatcacta
      121 ttgctaaaat cacgaattat gctgtcaatt aaatcgcgtc ggaaagcatc ttcctcagcc
      181 gatttcatta cgagtaattg cttaataaga gtctgcaatg aaaaatttat cttcatgcgc
      241 agtatctgga gcaccgcctg gccagtaatt tcatatttta tacgttcatt atcagatgtc
      301 ttcataacga cttcatagct aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc
      361 aacccaatta gtgcattata atgtttctgt tcctcttccc ctgtcgcttg ttccagtcgg
      421 caaagaagtt tggcacaaag cgactttcga ctcagattac gtccatcttt caaaatctca
      481 gtcacaattt ccccgagagt ttcctgctga gtaggcaagt tagcttttgc aaagtaacga
      541 gtgattgctc ccgcagaatc tgaagtcact gaattttgat gcatgctacg cctctgttaa
      601 aaatgttaat caatgtgaag tgaggtaaca atagcaaact tgtacgaaat atgtgtacag
      661 aatgataaat taat
//
LOCUS       DAPHDX010000387          673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPHDX010000387 DAPHDX010000000
VERSION     DAPHDX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.392"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..609)
                     /locus_tag="RH058_004694"
     CDS             complement(<1..609)
                     /locus_tag="RH058_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV3140110.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENN"
BASE COUNT          214 a          133 c          149 g          177 t
ORIGIN      
        1 attgttttcc gccaaatatc gcgttgcaga aagtcggcaa ataattaaca ctttgtctcc
       61 tttagtgaaa aattttcctt cttcaggctc cagtagtaaa taatgtattt ggccaaattg
      121 atcggtgagt tttccttcgc agggattacc tgaagtggct tgatgaccgg taattaatgc
      181 catgctacca atatattctt cttctgtaat agctgaacta tggtcgcgtg gaatccaggg
      241 cgcaactatt ttaccggtat aatgcaccgc aataatcgta aacagtaaac tgacaggaac
      301 cacgaacagg ttcgacagtg gtgattgcca gaccatgatg caggcatgct gcaacaggat
      361 gccaataaga ccaaaaaaac cagccagcaa gcagagaacg acgagagctg gcaatcttcc
      421 gatattgaga taatgaagtg cctggctaat atgacctgtg gttatggaat cataatggtc
      481 aagatgtgca tcaagagcac cggagagcat gtgaccgcag ataagcgcta aaatttctaa
      541 aaggccgatc aacagtacaa aggaaatagc gaaaagataa ggggtattat agtcggcgaa
      601 taaaatcata tgtcactcca atgtccattt agtgacgcaa tatttacttt gggatagttt
      661 ccttgcttac att
//
LOCUS       DAPHDX010000388          649 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPHDX010000388 DAPHDX010000000
VERSION     DAPHDX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.393"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..572)
                     /gene="fimE"
                     /locus_tag="RH058_004695"
     CDS             complement(<1..572)
                     /gene="fimE"
                     /locus_tag="RH058_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV3140111.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLIN"
BASE COUNT          188 a          167 c          132 g          162 t
ORIGIN      
        1 tcgtttatga gattatttct ttcccataat ccggcaaaac gagcagcatt actggcggta
       61 taacgcacag tatggcgaat atttcgatgc ccgagataat cctgaattaa acgagtatct
      121 gcaccacgct ccgccagttc ataaccgcaa gcatgcctta acatatgagg atgagtctgc
      181 gttacggttc cagcttcaat accggcatcg cgaataatgc gataggcctg ctggcgagaa
      241 agccgactcc cgcggcgaga aataaatata gcgtcagtcc ggtcagcgcc tttccagtta
      301 gcacgttcct gggtccatcg ttccacggct tcacgctcat caaaacgtaa cgggtgaacg
      361 gtagaaaatc cgttcttcag tcggcgaata tttattctac cttcattaag gtcaaggtcc
      421 tgataatgca gatcaagcag ttcactaata cgcatcccat gccgatatgc caacagaata
      481 agacaataat ctctggctcc cgttgccccg taacaaaccg cctgcatcat ggcctgaact
      541 tctttaccgg taagataacg acgtttactc acaatagtag tactcctaac tgagatatat
      601 ttgaatgaat acctatagga aacctcaatc ggtcaaaatt agcccaata
//
LOCUS       DAPHDX010000389          645 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPHDX010000389 DAPHDX010000000
VERSION     DAPHDX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.394"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>645
                     /locus_tag="RH058_004696"
     CDS             <1..>645
                     /locus_tag="RH058_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HDV3140112.1"
                     /translation="MPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQ
                     RWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFG
                     NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
                     YISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNR"
BASE COUNT          162 a          153 c          200 g          130 t
ORIGIN      
        1 gtatgcctgc gatcaatgcc aaacgtgttt accggatcat gcgccagaat gcgctgttgc
       61 ttgagcgaaa acctgctgta ccgccatcga aacgggcaca tacaggcaga gtggccgtga
      121 aagaaagcaa tcagcgatgg tgctctgacg ggttcgagtt ctgctgtgat aacggagaga
      181 gactgcgtgt cacgttcgcg ctggactgct gtgatcgtga ggcactgcac tgggcggtca
      241 ctaccggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc
      301 gcttcggcaa cgatcttccg tcgtctccag tggagtggct gacggataat ggttcatgct
      361 accgggctaa tgaaacacgc cagttcgccc ggatgttggg acttgaaccg aagaacacgg
      421 cggtgcggag tccggagagt aacggaatag cagagagctt cgtgaaaacg ataaagcgtg
      481 actacatcag tatcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg
      541 cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc tcgccacggg
      601 aatatctgcg gcagcgggct tgtaatgggt taagtgataa cagat
//
LOCUS       DAPHDX010000390          644 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPHDX010000390 DAPHDX010000000
VERSION     DAPHDX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.395"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(456..>644)
                     /locus_tag="RH058_004697"
     CDS             complement(456..>644)
                     /locus_tag="RH058_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3140113.1"
                     /translation="EQFGDLKFKYRNREFWCRGYYVDTVGKNTAKIQDYIKHQLEEDK
                     MGEQLSIPNPGSPFTGRK"
BASE COUNT          156 a          166 c          139 g          183 t
ORIGIN      
        1 tgcctggttg ctaaataatc tgctgataaa aaaaacagat ttgtacaaaa ttacgccatg
       61 ctcgctgtga gtaaccccgt tttgcgacgg tacatgctaa ccaatgaggt gcctgatttg
      121 atgtatcacc tgctggtgct gttgcaggtt caaatgctga atttaacaac aggatagtga
      181 ttctgcgcac gcggcatcag tgagttgcgg ggtaggcgga tgtgatgttg ctgtcacttc
      241 cggcaacaac tcatccgtta ttgcccggtg tgcaggttta tcgtgcctac aaattttctc
      301 aacatatgta agctggataa gatacgccgg catcgcatcc gacaaaaata aatatcctcc
      361 ggcatagccg gaggtttttc aaatgcgcct ataaggctct cttaccagcc gcgccctaac
      421 aggcgcacac gatctgacat ttgcatccaa cttcgttact tacggcccgt aaacgggctg
      481 cccggattgg gaatcgataa ctgctctccc attttatcct cttcaagctg gtgctttatg
      541 taatcctgta tcttcgccgt gttcttaccc actgtatcga cgtagtaccc tctgcaccag
      601 aactccctgt tcctgtattt gaatttcaaa tcaccaaact gctc
//
LOCUS       DAPHDX010000391          631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPHDX010000391 DAPHDX010000000
VERSION     DAPHDX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.396"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..456
                     /locus_tag="RH058_004698"
     CDS             <1..456
                     /locus_tag="RH058_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV3140114.1"
                     /translation="DEWANQLKHALSKEENLALLHGLTPDILDRIYAYAFDYHEKGNI
                     TDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPYDDYSVIYR
                     MGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
BASE COUNT          231 a          101 c          102 g          197 t
ORIGIN      
        1 gatgaatggg caaatcaact aaaacacgca ttatcgaaag aagaaaatct ggcattacta
       61 catggtttaa ctcctgatat ccttgataga atatatgcat atgcattcga ctaccatgaa
      121 aagggtaata taacggacgc agaaatttat tataaatttt tgtgcattta tgcgttcgaa
      181 aatcatgagt atctaaaaga ttttgcatca gtatgtcagc ccaaaaagaa atatcaacaa
      241 gcatatgacc tttacaaact aagttacaat tactccccgt atgatgacta ttcagttatt
      301 tatcgtatgg gtcaatgtca gattggggct aaaaatatcg ataacgcaat gcaatgtttc
      361 tatcacatta ttaacaattg tgaggatgat agtgttaaga gtaaagcgca ggcatatatt
      421 gaactcttaa acgataattc agaagataat ggctaagcat tacaatcatt tggttaaatt
      481 tgctggttat agtgcagtta accactataa ccagcacctc ttatgttttc ttaaatcagc
      541 atcttaaaac agttaagagc atactataaa tcattacttt tcaatgcgtt agagaacaat
      601 ctactttata aaaattgaat aaggctattt c
//
LOCUS       DAPHDX010000392          626 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPHDX010000392 DAPHDX010000000
VERSION     DAPHDX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.397"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            165..240
                     /locus_tag="RH058_004699"
     tRNA            165..240
                     /locus_tag="RH058_004699"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Ile,seq:cat)
BASE COUNT          180 a          113 c          147 g          186 t
ORIGIN      
        1 ttgttgactg cgagtatcga ctttgcgaat ggaagcggtg tcgggtttta tgtcgcgtgt
       61 aatgacgagc aaggatgaat gagaacgcat aaaaatctgt tatttacatc atatgatcat
      121 catgtttatt gcgtggttta taagtattgt tattgcacac tcaaggccct ttagctcagt
      181 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca agggccacca
      241 aatcattatt tacgtatcag gaagagttaa gggtacaagg ggtactcgat ggcggtctat
      301 atttcatgac taacgccatt tcaaggaaga agtgcggtgg gtggtttcga atgagggctg
      361 gtgttttggt acgtggcacg ccactgcaat gtatttatcc atcagtactc attcaaaagt
      421 acctggaaaa aatgcctgct atgccaagcg ggcaaattgc ttacatgtgg aattaagtaa
      481 ttaacgataa agccagagta ttatcattta ttatacccct taatttaatc aggttaatta
      541 ctcaaatcac tgaactattc tctgttcgct cactgatgat gagtgagtac cacaaccaat
      601 gtatgtagaa caatgccgta gagtaa
//
LOCUS       DAPHDX010000393          621 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPHDX010000393 DAPHDX010000000
VERSION     DAPHDX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.398"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>621
                     /gene="ygeH"
                     /locus_tag="RH058_004700"
     CDS             <1..>621
                     /gene="ygeH"
                     /locus_tag="RH058_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HDV3140115.1"
                     /translation="FLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLLESAGHVVLKD
                     MIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINE
                     DNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQ
                     NSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPV"
BASE COUNT          206 a          110 c          107 g          198 t
ORIGIN      
        1 attttcttga gggattaacg ctcacggaag atggaattct tactcaagga aatgagcaag
       61 tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc gctggtcatg
      121 ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt agtgacgagt
      181 ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc tatgatcgtt
      241 gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc aaaactaaaa
      301 ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact acttcattga
      361 atacactgga tccattaata cttaatcagg aattagtgca aatcatttca aataaaaaaa
      421 tcgatggtct ctatacctat ccgatggctg cgacaaattt ttgtaatgat cacatatctc
      481 aaaattcatt cttgagcaga ttcaagccag attatttcgt tacaggaaga ataaaccaga
      541 ataatgcagt gaacacttta tacattgagt tgatcgacgc taaaaacctt ttcctcatcg
      601 ccagtaatca tctccctgtt g
//
LOCUS       DAPHDX010000394          612 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPHDX010000394 DAPHDX010000000
VERSION     DAPHDX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.399"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..348
                     /locus_tag="RH058_004701"
     CDS             <1..348
                     /locus_tag="RH058_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDV3140116.1"
                     /translation="VSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKV
                     YFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKT
                     PKEIIERGVALTD"
     gene            358..612
                     /locus_tag="RH058_004702"
                     /pseudo
     CDS             358..612
                     /locus_tag="RH058_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          188 a          148 c          145 g          131 t
ORIGIN      
        1 gtctcagtaa atcaggctct taccgacaaa ttcctgagtt taccgtcaga actcagaaaa
       61 tcactgacat gggacagagg aatggaactg gccagacatc tagaatttac tgtcagcacc
      121 ggcgttaaag tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaac
      181 acaaatgggc taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa
      241 catgaactag atctggttgc tgctcagcta aacaacagac cgagaaagac actgaagttc
      301 aaaacaccga aagagataat tgaaaggggt gttgcattga cagattgaat ctacaaccgc
      361 gctcttgatg tcagactccc tgaacagttc tcgataatcg ggaaactcag agcgcattat
      421 cctgtggtca cactctgcca tgtgttcggg gttcatcgca gcagctacag atactggaaa
      481 aaccgtcctg aaaaaccaga cggcagacgg gctgtattac gcagtcaggt acttgagcta
      541 catggcatca gccacggttc ggccggagca agaagcatcg ccacaatggc aacccggaga
      601 ggctaccaga tg
//
LOCUS       DAPHDX010000395          594 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPHDX010000395 DAPHDX010000000
VERSION     DAPHDX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.400"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            14..>594
                     /locus_tag="RH058_004703"
     CDS             14..>594
                     /locus_tag="RH058_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140117.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSIFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTK"
BASE COUNT          238 a          120 c           87 g          149 t
ORIGIN      
        1 aataaggaat gttatgtcaa ttgactttac cccaggtata ataaatacat atcacggcga
       61 tatttataac tgcacaacaa ataccgataa tgttaaaacg ccagacacac cgaagtggcc
      121 ttgtgataat tgggaagaac aacaacccat taattccata ttctctggag aaggatacaa
      181 ccctgaacaa tttgatttag cgcaacaaca actccaacaa attaatgcat gccacacgaa
      241 caccacatat acaaatgcag actactcaaa agttgtggtt caacttgtaa gtcttattaa
      301 taacattgaa acgataagtt cgacaccact tacgcaacag acacaatcaa tacttaatca
      361 gattaataac attagatatg aaaaaaacaa aagtgctgaa tgtcgaatca ttgttgtcgc
      421 taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg gaatacccat
      481 aacattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac aacgagctga
      541 cttaccaact aatataaaag atattcaaag cctgtatcaa aaaatgacga agtt
//
LOCUS       DAPHDX010000396          581 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPHDX010000396 DAPHDX010000000
VERSION     DAPHDX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.401"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>581
                     /locus_tag="RH058_004704"
     rRNA            <1..>581
                     /locus_tag="RH058_004704"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          177 g          113 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca c
//
LOCUS       DAPHDX010000397          564 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPHDX010000397 DAPHDX010000000
VERSION     DAPHDX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.402"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>564
                     /locus_tag="RH058_004705"
     CDS             <1..>564
                     /locus_tag="RH058_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140118.1"
                     /translation="LKEGYLNTIALFARDYSASFASKIHGAFLAFIKEEQCVYVSKAS
                     VLNFRSKKHVKDELLFRLRIFITKWYSLGYNGIASEAVQIMKTWKLVNGKPGDVVKRK
                     EPIQGPLTDIELQEFNEGAIRAYEKKEISLFMLTMALIISHTGRRPLQVTQMKITDIL
                     KVSKEEGEIYYLLNIPRIKQGLGFREE"
BASE COUNT          205 a           82 c          115 g          162 t
ORIGIN      
        1 tttaaaagaa ggatatctaa atacaattgc actatttgcg agggattata gcgcaagttt
       61 tgcctccaag atacatggag catttcttgc ttttataaag gaagagcaat gcgtatatgt
      121 cagtaaagct tcagttttaa atttcagaag caaaaagcat gttaaggatg aattattatt
      181 tcgtctgagg atatttatca ctaaatggta ctctcttggc tacaatggaa tagcttcgga
      241 agctgtacag ataatgaaaa catggaaact tgttaacgga aagccaggtg atgtcgttaa
      301 aagaaaagaa cccatacaag gtcctttgac tgatattgaa ttgcaagaat ttaacgaggg
      361 agcaataaga gcgtacgaga aaaaagaaat atctcttttt atgttaacta tggcacttat
      421 tatcagccac acaggtcgta gacccttgca agtaactcag atgaagataa cagatatcct
      481 taaggtttca aaagaagagg gtgagattta ttatctttta aatataccac gaattaaaca
      541 aggcttagga ttcagagaag agtt
//
LOCUS       DAPHDX010000398          563 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPHDX010000398 DAPHDX010000000
VERSION     DAPHDX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.403"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(31..432)
                     /locus_tag="RH058_004706"
     CDS             complement(31..432)
                     /locus_tag="RH058_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDV3140119.1"
                     /translation="MSESIKILNNIRTLRAQAREFSLETLDEMLEKLTTVVEERRQEY
                     VSYRKEREERQAKLEAFRLQLLEDGIDPADLLSGLSSSSKPKSNREPRPAKYKYIDEN
                     GEQKTWTGQGRTPKAIATAIEAGKALEDFAI"
BASE COUNT          150 a          123 c          109 g          181 t
ORIGIN      
        1 cttattttat taaaagttac ttattaaaga ttagatcgca aaatcttcca gagctttccc
       61 agcctcaata gctgtggcaa tagctttagg agtgcgacct tgaccagtcc aagtcttctg
      121 ttcaccgttt tcatcgatgt atttgtactt agccggtcgt ggttcacggt tggattttgg
      181 tttagaggac gaactcaaac cagagagcag atctgccggg tcaatgccat cctcaaggag
      241 ttgcaggcgg aaagcttcaa gcttggcctg acgctcttcc ctctccttgc gataggaaac
      301 gtattcctgc cgacgctctt ctaccacagt agtcagcttc tcaagcattt cgtcaagagt
      361 ttcaagagaa aactcacgcg cctgagcacg aagcgtacga atgttgttta gtatttttat
      421 agattcgctc atatgtcttc cttcctggtg atgtttagag cattgcttta ttaatgattt
      481 tctttatcaa atgcaagagt ttacattaaa aacacagaac aacaaacatt tagttacaat
      541 aatcttcgtt aatcttattc att
//
LOCUS       DAPHDX010000399          552 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPHDX010000399 DAPHDX010000000
VERSION     DAPHDX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.404"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..375)
                     /locus_tag="RH058_004707"
     CDS             complement(<1..375)
                     /locus_tag="RH058_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140120.1"
                     /translation="MSYQYSQEAKERISKLGQSEIVNFINEISPTLRRKAFGCLPKVP
                     GFRAGHPTEIKEKQKRLIGYMFQSHPSSEERKAWKSFSLFWQFWAEEKIDKSFSMIDN
                     LGLKENSGSIFIRELAKNFPKVA"
BASE COUNT          171 a          112 c           86 g          183 t
ORIGIN      
        1 agcaacttta ggaaagtttt tagcaagctc tctaataaaa atagagccag agttttcttt
       61 taatcctaaa ttatcaatca tactaaatga tttgtcaatt ttctcttcag cccaaaactg
      121 ccaaaaaaga gaaaaacttt tccatgcttt tctctcctca gaggaaggat gtgactggaa
      181 catataccca atcaatcttt tctgtttttc tttaatttca gtgggatgtc ctgccctgaa
      241 tcccggtact tttggtaaac aaccaaaagc tttacgtcgt aaagttggag aaatctcatt
      301 gataaagtta acaatttcgg attgtcccaa cttagagatc cgttcctttg cctcttggct
      361 atattggtaa ctcattcgaa ggcaccttat ctaaagcggt tacattcaaa tccattggtc
      421 ttaacttctt ttaccaatgc tctataatgc ggtaataagt gtggttccgg ctgcaaatcc
      481 actaatttct caactaataa aaaatcatta tttaaactaa aaccatggag ggtgtgcccc
      541 atttgaagta ta
//
LOCUS       DAPHDX010000400          550 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPHDX010000400 DAPHDX010000000
VERSION     DAPHDX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.405"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>550)
                     /locus_tag="RH058_004708"
     CDS             complement(<1..>550)
                     /locus_tag="RH058_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3140121.1"
                     /translation="PLIRVETENGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRL
                     LDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLA
                     LISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQ
                     GRYITQDPIGLAGGWNLYNYPL"
BASE COUNT           81 a          195 c          141 g          133 t
ORIGIN      
        1 ttcagtgggt aattatacag gttccatccc cccgccagcc caatggggtc ttgtgtaata
       61 tacctcccct gtagcgggtc atagtaccgg ttccggttat agtacagccc tgattcctca
      121 tcatgctgct gccccggcag acggtatggc tggtgcaggt aatacgggtt ctcttcatta
      181 agctggttgc cccattcatc atactccccg cgccacgccg tattgccgtc ttcgctgatg
      241 agcgccagcg gcaggccccg gtggtcgcag tggtaaaaat gaacttttcg cgccggtgtg
      301 tattccggct ccacctgtct ggccagttgc tccaccgtca gcccgcactg cgcaagccac
      361 gcccggcttt cactgctcac gcggtctgcc cggatttctt cctccagcct gtccagcagc
      421 cgcaccagtt cagccggaaa taccacgccg tgcccgtcct cgctcccttc ctgctgcagc
      481 ttctccgcca ggctgcggcg ctgcgctttc tcccgctcgc cgttctctgt ctcgacccgg
      541 atgagcggcg
//
LOCUS       DAPHDX010000401          544 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPHDX010000401 DAPHDX010000000
VERSION     DAPHDX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.406"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..504
                     /locus_tag="RH058_004709"
     CDS             148..504
                     /locus_tag="RH058_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140122.1"
                     /translation="MKRALSVLLSIVPLTTIAATIPDGKTEKPFVIDGEVICLKEIQK
                     QIPPYLKSFEEESGVKYKYNVKSGLSYKDTVGLRLQINRVDSGLDMRVADLDYYWLQE
                     RGSLCQPTYMVVDRSQ"
BASE COUNT          173 a           90 c          124 g          157 t
ORIGIN      
        1 caaggaagta cggaaattcg tcaagtaatt attttgtatc tgtacttaat gataatacta
       61 taaaagtaat tgatgttgaa tttaaccata atgcaaccat tacacaaatt tgttctaaac
      121 aatgattata ttcaagggga agaacagatg aaaagagcat tatcagtact gttgtcgata
      181 gttccattaa ccacaattgc ggctactatt cctgacggta aaactgaaaa gccgttcgta
      241 attgatgggg aagttatttg tttgaaagaa attcagaagc aaatcccccc ttatctcaaa
      301 tcttttgaag aagaaagcgg ggttaagtac aagtacaatg ttaaatctgg tttatcgtac
      361 aaagatactg tagggctgcg gttacagatc aatcgtgtgg attcggggtt ggatatgcgt
      421 gttgcggatc ttgattacta ctggttacag gaacgcggca gcctgtgtca accaacatac
      481 atggtagttg atcgttctca atgagcacgg gctagataag gtcgcggaag cggccttcgt
      541 cgag
//
LOCUS       DAPHDX010000402          528 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPHDX010000402 DAPHDX010000000
VERSION     DAPHDX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.407"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            58..519
                     /locus_tag="RH058_004710"
     CDS             58..519
                     /locus_tag="RH058_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140123.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          196 a           90 c           95 g          147 t
ORIGIN      
        1 aaaagtgata tacagacgct aaaaacagaa tgttattgcc ttctagcgga atgccatatg
       61 tctttggcac ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc attagagctt
      121 ttagattatg tatcagacat aaccactgtc gatggaaaaa ttttagctat tatgggactg
      181 ataactggtc tgtctggaca agcaaaagta tctcatatct tatttgaaca ggctaagata
      241 cactcaactg atatagcctc tctctactac tatagggcac tagtccactt tcataatgaa
      301 aaaattgaag aggcaaggat ttgtatagac aaatcactac aactcgaacc cagaagacga
      361 aaagcagttg tgataaaaga atgtgtagat atgtatgtac ctaacccgct caaaaacaac
      421 attaaactct actataaaga aactgagagt gaaagccatc gagttataat tgacaacatt
      481 ttgaaattaa agcagctgac gagaatttgt atgcgataaa tttttatt
//
LOCUS       DAPHDX010000403          522 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPHDX010000403 DAPHDX010000000
VERSION     DAPHDX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.408"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..>522
                     /locus_tag="RH058_004711"
     CDS             32..>522
                     /locus_tag="RH058_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDV3140124.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLKTMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSS"
BASE COUNT          127 a          148 c          131 g          116 t
ORIGIN      
        1 gaaggtgcga acaagtccct gatatgagat catgtttgtc atctggagcc atggaacagg
       61 gttcatcatg agtcatcaac ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca
      121 gaccagaaaa gagattttct tgtcccgcat ggagcagatt ctgccatggc aaaacatggt
      181 ggaagtcatc gagccgtttt accccaaggc tggtaatggc cggcgacctt atccgctgaa
      241 aaccatgcta cgcattcact gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga
      301 agatgctctg tacgaaatcg cctccatgcg tctgtttgcc cggttatccc tggatagcgc
      361 cttgccggac cgcaccacca tcatgaattt ccgccacctg ctggagcagc atcaactggc
      421 ccgccaattg ttcaagacca tcaatcgctg gctggccgaa gcaggcgtca tgatgactca
      481 aggcaccttg gtcgatgcca ccatcattga ggcacccagc tc
//
LOCUS       DAPHDX010000404          515 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPHDX010000404 DAPHDX010000000
VERSION     DAPHDX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.409"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          143 a          110 c           99 g          163 t
ORIGIN      
        1 aattactgct ggtacgacct taatgtctgc tacatcattt tgtcatacaa aatatttctt
       61 tctgatacat tctaagttct atcacagctt cgccagtcaa tgttattttt tctgtatata
      121 agcttgaaat tctgtatgaa atggtgggaa aatgataagg cacataggga ttagcctatt
      181 tttgtttatg atcattgccg gcgtttttcg ccggcttttt tatgtctcca gagttattac
      241 ttttcgatat tacggtgctt ttcaactggt ctgcctgtaa agccctggaa aaaacgaaag
      301 gtatttatga taaccgaatt ttcgcgatga cgccctgacc acattatgat ttgagtccgg
      361 acatctcatt gtcgttatac aagaagcatg attatcaatc acggacatta cgacaatgca
      421 gcactgcata ccagaactgg atacctgcgg gtccggcgac aactctgcct ccctcatata
      481 taacaccagc acacggacga taaagcagta caggg
//
LOCUS       DAPHDX010000405          511 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPHDX010000405 DAPHDX010000000
VERSION     DAPHDX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.410"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>511
                     /locus_tag="RH058_004712"
     CDS             <1..>511
                     /locus_tag="RH058_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HDV3140125.1"
                     /translation="MKELGLISCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWC
                     GDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVM
                     FHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWIPVVGYVS
                     FSEAAHAIT"
BASE COUNT          122 a          125 c          160 g          104 t
ORIGIN      
        1 tcatgaaaga gctggggctg atcagctgtc agcagccgac tcaccggtat aaacgtggtg
       61 gtcatgaaca tgttgctatc cctaactacc ttgaacggca gttcgccgtg accgagccaa
      121 atcaggtgtg gtgcggtgat gtgacctata tctggacggg taagcgctgg gcgtacctcg
      181 ccgttgttct cgacctgttc gcaagaaaac cagtgggctg ggccatgtcg ttctcgccgg
      241 acagcaggct caccatgaaa gcgctggaaa tggcatggga aacccgtggt aagcccggcg
      301 gggtgatgtt ccacagcgat cagggcagtc attatacgag caggcagttc cggcagttat
      361 tgtggcgata ccagatcagg cagagtatga gccggcgcgg aaactgctgg gataacagcc
      421 caatggaacg cttcttcaga agtctgaaga acgaatggat accggtggtg ggttacgtaa
      481 gcttcagcga ggcagctcac gccataacgg a
//
LOCUS       DAPHDX010000406          506 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPHDX010000406 DAPHDX010000000
VERSION     DAPHDX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.411"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>506
                     /locus_tag="RH058_004713"
     CDS             <1..>506
                     /locus_tag="RH058_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV3140126.1"
                     /translation="GRIHHYKGLDILIDAFEILNKKNNRYSLSIYGSGNISPYQKKIL
                     ELSNITVHNRWIDDVEIPSIISSHDVCVIPYRDASQSGVIPTVMTCGVPLVVTPVAGL
                     KEQVVHLQTAIFAEEVSSDSVARAIDSLVKDISLYEKISKNALLYAQEEMGWEKAAYR
                     SVEALISI"
BASE COUNT          154 a           85 c          109 g          158 t
ORIGIN      
        1 ttggtagaat tcatcactac aaagggctgg atattcttat tgatgcattt gaaatactga
       61 acaaaaaaaa taacaggtat agtctttcta tctacggtag tgggaatatc tctccatatc
      121 aaaagaaaat tctagaacta agcaatatta cagtgcataa tcgttggatc gatgatgtag
      181 aaattccttc aatcatctct agccatgatg tctgtgttat tccttatcgg gatgcaagtc
      241 agtcaggagt aattcctacg gtaatgactt gtggtgtgcc tcttgttgtg accccggttg
      301 ctgggcttaa agagcaggtt gttcacttac aaacagcaat atttgcggaa gaggtttctt
      361 ctgattccgt tgcaagagca atagattcat tggttaagga tatatctttg tatgaaaaga
      421 tatcaaagaa tgctctacta tacgctcagg aagagatggg atgggaaaaa gccgcatatc
      481 gttctgtgga agctcttatc tcaatt
//
LOCUS       DAPHDX010000407          498 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPHDX010000407 DAPHDX010000000
VERSION     DAPHDX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.412"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..>498
                     /locus_tag="RH058_004714"
     CDS             45..>498
                     /locus_tag="RH058_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV3140127.1"
                     /translation="MLPESNEFPIIQIEIAKIKFLNPRTRNKVVHEEIKESIKKRGLS
                     KPISVRAIDEDDFKYALICGQGRIEALVALGETIIPAIIRDVSEEDAYVMSLVENIAR
                     RRPRSNELLQIIKDMKNRGLSDSEISEITGYSSNWVSSINMLLDKGEHK"
BASE COUNT          193 a           71 c          108 g          126 t
ORIGIN      
        1 taaacttcta caaattatta ctcgcatgaa agtgagggaa ctatatgctg cctgaatcaa
       61 atgaattccc aataatccaa attgaaattg ccaaaataaa atttcttaac ccgcgaacta
      121 gaaataaggt agtgcatgaa gaaattaagg aaagcataaa aaaaagaggg ttaagcaagc
      181 ctataagcgt aagagcgatt gatgaagacg acttcaaata tgctttaatt tgtggccaag
      241 ggagaataga ggcgctcgtt gcattaggtg aaaccattat tccagcaatt ataagagatg
      301 tatcagaaga agatgcttat gttatgagtt tggttgaaaa cattgcaagg aggagaccac
      361 gttccaatga gttattacag ataataaaag atatgaaaaa cagaggactt tcagactccg
      421 aaataagtga gattactggg tattcatcga actgggtgag cagtataaat atgcttcttg
      481 ataagggaga gcataaac
//
LOCUS       DAPHDX010000408          494 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPHDX010000408 DAPHDX010000000
VERSION     DAPHDX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.413"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>494
                     /locus_tag="RH058_004715"
     CDS             <1..>494
                     /locus_tag="RH058_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDV3140128.1"
                     /translation="SLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRA
                     NDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGK
                     KCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQ
                     LARE"
BASE COUNT          150 a           88 c          111 g          145 t
ORIGIN      
        1 tcactgtatt tattaaaaaa tacagaggag attcaacgtg ccgtgattct gaaaatggaa
       61 atgatgccat ttttagactc ggtgggactg gtacttgatg ataataaata ttatctcttt
      121 tcgcggaggg cgaatgataa aatcgttgtt tatcatcagg aacaagtaaa tggaccgctt
      181 gtcgacgagt cagggcgggt tatttttgcc gattttaacc catcgaaacg accgtggtcg
      241 gtggcttcag atgactctaa caacagctgg aatccggcat acaattgctt tgatcgtccg
      301 ggtaaaaaat gtatctcttt tacgctacgt atcaacggca aagatcacga tttgttagcg
      361 gtagataaaa ttcatgtcga tttaaactgg cggtatctga acgagtatct tgatcaaatc
      421 agcgctaatg atgaagttct atttttgaaa caaggtcatg agatcattgc caagaatcaa
      481 ctcgctcgtg aaaa
//
LOCUS       DAPHDX010000409          491 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPHDX010000409 DAPHDX010000000
VERSION     DAPHDX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.414"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..458
                     /locus_tag="RH058_004716"
     CDS             <1..458
                     /locus_tag="RH058_004716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140129.1"
                     /translation="ERIYNNKKLEEALKRVTYSGLTSEATLIINQTIQECKSLGKYKL
                     PVKDVFYIVKHATELSKVQVKYYLTLTRSDQMKKFAASESSIEKYKLACTAVAKALDK
                     FVEEGGTLCGLKKALPGKPLTDEQKNAVQELLNSNASAKDLIAYLRTIV"
BASE COUNT          187 a           88 c           94 g          122 t
ORIGIN      
        1 aagaaaggat ttataacaat aaaaagttag aagaagcact aaaacgtgtt acttacagtg
       61 gcctaacatc agaagcaact ctaataatta accagacgat tcaagagtgt aaatcattgg
      121 gaaagtacaa gttacctgtt aaagatgtct tctacatcgt caaacacgct acagagcttt
      181 caaaagtaca ggttaagtat tatcttacct taacccgttc agatcagatg aagaaatttg
      241 cagcaagtga atcatctatc gagaagtaca aattagcctg taccgcagtt gctaaagcat
      301 tggataagtt tgtagaagaa ggtggtacgc tatgtggact aaagaaagca ttgcctggta
      361 agccattaac cgacgaacag aagaacgcag tacaggaact attgaacagt aatgcctcag
      421 cgaaagatct gattgcatat ctacgaacca ttgtttaatc gtacctataa ccgtaactta
      481 aaactaagaa a
//
LOCUS       DAPHDX010000410          488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.415, whole genome shotgun sequence.
ACCESSION   DAPHDX010000410 DAPHDX010000000
VERSION     DAPHDX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.415"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..223
                     /locus_tag="RH058_004717"
     CDS             47..223
                     /locus_tag="RH058_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDV3140130.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
BASE COUNT          140 a           97 c           99 g          152 t
ORIGIN      
        1 aagagaacct cgttaatgat tggtcttgct cattgttaat aacaaaatgt tgaatttacc
       61 gattaggaaa ctttttccta cgcggattgc ctccagctat ggcatgaact ttgagtttat
      121 gccagaactg aagtggggct tcggctaccc tggcgcgatt atctttatga tcctcgcggg
      181 cctgacaccg tatctgtact ttaagcgtaa gaactggctg taactatacg atgtcgctgt
      241 gagtaagtta gtgtgctgac agcgacattt tagccgaggg aatcgcagat aacataattg
      301 ttgtgaataa tatccatgcc acgttgagtt ggacgcttaa tgactcacat ctcacccctt
      361 caattgctgc agagataata gaaaaaagtg tcaagcccaa tgaaatcaat acaaagtaga
      421 ttgccttaca tccagtatct cttattcttt ttgctccaat attgatattt acataggtat
      481 tattaata
//
LOCUS       DAPHDX010000411          487 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPHDX010000411 DAPHDX010000000
VERSION     DAPHDX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.416"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(2..>487)
                     /locus_tag="RH058_004718"
     CDS             complement(2..>487)
                     /locus_tag="RH058_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV3140131.1"
                     /translation="GLLFQLFSVRNLPILALIMGLSFALYGLIRKFIHYDAITSVTLE
                     TFWSLPVSLILFIYTDPDVTTPSEIPCILYIMTAPVTVIPLVLFAVALNHTSLIVTGL
                     AQYIEPSMQFLIAVFIFGEVIHYDELFCFSAVWLGLLLCIIESCYRHYRQIPVCKHHT
                     R"
BASE COUNT          174 a           87 c          112 g          114 t
ORIGIN      
        1 attaccgggt atggtgttta cagacaggaa tctgacggta atgacgataa cagctttcga
       61 taatgcacaa caataacccc aaccagactg cagaaaagca gaataattca tcataatgta
      121 taacttcacc gaatataaac acagcaatca ggaattgcat agaaggttcg atatattgag
      181 caaggccggt tacgataagt gatgtatgat tcagtgcaac agcaaacagc accaacggga
      241 taacagtgac tggtgcagtc ataatgtaca gaatacatgg gatctcagag ggcgtggtta
      301 catccgggtc ggtatatata aaaaggataa gtgaaacagg taatgaccag aatgtttcaa
      361 gcgtgactga ggttatagca tcatagtgaa tgaattttct tatcagtccg tataatgcaa
      421 atgaaagccc catgattagt gccagtatgg gaaggttgcg taccgaaaac aactgaaata
      481 gcagtcc
//
LOCUS       DAPHDX010000412          485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPHDX010000412 DAPHDX010000000
VERSION     DAPHDX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.417"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>485)
                     /locus_tag="RH058_004719"
     CDS             complement(<1..>485)
                     /locus_tag="RH058_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDV3140132.1"
                     /translation="NFLMDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTF
                     ECKEIGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQLTQS
                     NFREYQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNMTAVMFDHVRISTGNFKDC
                     I"
BASE COUNT          168 a           92 c           73 g          152 t
ORIGIN      
        1 taatgcaatc tttaaaattc cctgtcgata ttcgcacatg atcaaacata acagctgtca
       61 tattcgattt aataaatgat atttctttgg cagacagaaa ctctgctctg gtgaagtgcg
      121 aagcgtaaag ataacaatta tcgaagtttg aatttctaat ctgatattcg cgaaaattac
      181 tctgagtaag ttgactctgc gagaagtcaa ctgtatcaag aatatttttg ctgaaatcga
      241 cacattcacc actcgctttg ataaaaatca cattgtgtaa acgtcgacat ctaaatatga
      301 cattatcaac tattgcagta gtaaaattgc aatccccaat ttctttacat tcgaatgtta
      361 catgttgtag atttgatgat aaaaaagaca ccttcgatat aacgtcacct ttaaaatgac
      421 aacttgttaa atccaaatca gacaggtcga tatacccctg cgcatcttta tccatcagaa
      481 aattg
//
LOCUS       DAPHDX010000413          482 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPHDX010000413 DAPHDX010000000
VERSION     DAPHDX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.418"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>482)
                     /locus_tag="RH058_004720"
     CDS             complement(<1..>482)
                     /locus_tag="RH058_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1705 domain-containing protein"
                     /protein_id="HDV3140133.1"
                     /translation="LFFFVVSVITTIACGYTEKNTTGNVLLLFFLLLLAHRNTLTSIT
                     ALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEFTGMIPVYHFLVSVTVLVF
                     MVIFWQTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLREMQHYKQLSQHG
                     A"
BASE COUNT          149 a          118 c          107 g          108 t
ORIGIN      
        1 gccccgtgct gactcagttg tttataatgc tgcatttcac gcaatgtgtc agttgtcccc
       61 acaactgttc ctttaaccat ccgcaacggc cagctgttta ctgagcataa tacgaacagc
      121 agcagtgcca gccagttacg gtgaccgcgg tggtgtgtct gccagaaaat caccatgaat
      181 accagaaccg tgacactaac cagaaaatga taaacaggaa tcatcccggt aaactctgca
      241 gcctcatcag ccgtggtctg cagcaacgcg acaataaaac tgttgttgat tttaccgtac
      301 gtcatacccg caggcgcata cagtgcacaa cagaacagaa ataacagcgc tgtaatggat
      361 gtgagggtat ttctgtgtgc aagaagcaga agaaagaaca gcagcaacac attcccggtg
      421 gtattcttct cagtgtatcc gcatgcaatt gtggttatga cagaaacaac aaaaaagaat
      481 aa
//
LOCUS       DAPHDX010000414          469 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPHDX010000414 DAPHDX010000000
VERSION     DAPHDX010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.419"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>469
                     /locus_tag="RH058_004721"
     CDS             <1..>469
                     /locus_tag="RH058_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3140134.1"
                     /translation="DYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHK
                     QQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHE
                     GVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDL"
BASE COUNT          123 a          113 c          110 g          123 t
ORIGIN      
        1 tgactacatt ctgtttgact ccgcatgggt tggctatgag caatttatcc cgatgatggc
       61 tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca
      121 atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag
      181 ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat
      241 gcacgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat
      301 gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc
      361 ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga
      421 tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctg
//
LOCUS       DAPHDX010000415          455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPHDX010000415 DAPHDX010000000
VERSION     DAPHDX010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.denovo.422"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..421
                     /locus_tag="RH058_004722"
     CDS             <1..421
                     /locus_tag="RH058_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV3140135.1"
                     /translation="QVFSADIHGRGVRVLDGNTIDVMLSQHPVRVRLVNIDAPEKKQE
                     YGRWSEKIMKSLVAGKTVTVTYFQRDHYGRILGQVYAPDGMNVNQFMVRAGDAWVYEQ
                     YNTDPVLPVLQGEVRQQKRGLWADSAPVPPWVWRSRK"
BASE COUNT          112 a           89 c          123 g          131 t
ORIGIN      
        1 tcaggtattt tctgctgata ttcatggacg gggcgtgcgt gttctggatg gcaataccat
       61 tgacgtcatg ttatctcagc atccggtgcg tgtccgtctt gttaatattg atgcacctga
      121 aaagaaacag gaatatggac ggtggtctga aaaaataatg aaatctctgg ttgcgggaaa
      181 aacggttact gtcacatatt ttcagcgaga tcattatggt cgtattctcg ggcaggttta
      241 tgcaccagac ggaatgaatg ttaatcagtt tatggtccgt gcaggcgatg catgggttta
      301 tgagcagtac aacaccgatc ctgttcttcc tgtcctgcag ggagaagtac gacagcagaa
      361 acgtggtctg tgggcagaca gcgctcctgt tccgccctgg gtatggcgct cacggaaata
      421 atgatatttc tgtggctatt gagttctcat tgaaa
//
LOCUS       DAPHDX010000416        18131 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.guided.088, whole genome shotgun sequence.
ACCESSION   DAPHDX010000416 DAPHDX010000000
VERSION     DAPHDX010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.guided.088"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="RH058_004723"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="RH058_004723"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV3140136.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="RH058_004724"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="RH058_004724"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV3140137.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="RH058_004725"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="RH058_004725"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV3140138.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="RH058_004726"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="RH058_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV3140139.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6382..6696)
                     /locus_tag="RH058_004727"
     CDS             complement(6382..6696)
                     /locus_tag="RH058_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV3140140.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6693..7007)
                     /locus_tag="RH058_004728"
     CDS             complement(6693..7007)
                     /locus_tag="RH058_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3140141.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="RH058_004729"
     CDS             complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="RH058_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV3140142.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="RH058_004730"
     CDS             complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="RH058_004730"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV3140143.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10082..11890
                     /gene="frdA"
                     /locus_tag="RH058_004731"
     CDS             10082..11890
                     /gene="frdA"
                     /locus_tag="RH058_004731"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV3140144.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11883..12617
                     /gene="frdB"
                     /locus_tag="RH058_004732"
     CDS             11883..12617
                     /gene="frdB"
                     /locus_tag="RH058_004732"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV3140145.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12628..13023
                     /gene="frdC"
                     /locus_tag="RH058_004733"
     CDS             12628..13023
                     /gene="frdC"
                     /locus_tag="RH058_004733"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV3140146.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13034..13393
                     /gene="frdD"
                     /locus_tag="RH058_004734"
     CDS             13034..13393
                     /gene="frdD"
                     /locus_tag="RH058_004734"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV3140147.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            13456..14589
                     /locus_tag="RH058_004735"
     CDS             13456..14589
                     /locus_tag="RH058_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV3140148.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14678..15211
                     /gene="blc"
                     /locus_tag="RH058_004736"
     CDS             14678..15211
                     /gene="blc"
                     /locus_tag="RH058_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV3140149.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15208..15525)
                     /gene="sugE"
                     /locus_tag="RH058_004737"
     CDS             complement(15208..15525)
                     /gene="sugE"
                     /locus_tag="RH058_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3140150.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15707..15853)
                     /gene="ecnB"
                     /locus_tag="RH058_004738"
     CDS             complement(15707..15853)
                     /gene="ecnB"
                     /locus_tag="RH058_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV3140151.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15964..16089)
                     /gene="ecnA"
                     /locus_tag="RH058_004739"
     CDS             complement(15964..16089)
                     /gene="ecnA"
                     /locus_tag="RH058_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV3140152.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16141..16707)
                     /gene="efp"
                     /locus_tag="RH058_004740"
     CDS             complement(16141..16707)
                     /gene="efp"
                     /locus_tag="RH058_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV3140153.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16749..17777
                     /gene="epmB"
                     /locus_tag="RH058_004741"
     CDS             16749..17777
                     /gene="epmB"
                     /locus_tag="RH058_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV3140154.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLHQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4529 a         4780 c         4647 g         4175 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtac
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccg gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ccgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgaacc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca
     3781 gcgccgattt gccgaaagac atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgc caggccacca
     3901 gccagacgtt acaggttcgg caggcgctga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggaact gaccaagctg aaagtctcca
     4261 acgggcaact tgaggatgcg ctgaaagagg tgaatgaagc gacgcatcgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccact ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc actgtatctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcgccgttgg ttgccattct ggcgtcggct gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgtcgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttctctgtg ggatgtcact tccactgtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcagt gctaattgcc attctggtat ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgccagcgct gctggaactg gcgattttgc agcacctgga tttaactccg ggtacgggtt
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggtctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtga caattcgcga tctcaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gataccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taagtttgtg
     6361 tgccggatga accaatccgg ctcacaaaca aaactgatag cgcagaaaaa actgccacag
     6421 ccagtagccg aaaatagcca gcgcggtggc gtacataaac cagccgcgtt tttttcgcca
     6481 caaagcgaac cagagaaaag ggaaaaagag cggtaacgtc attatcacac caacatcacg
     6541 ggtatcgtct tcttcgagat acatcaacac atcgcagacg ttgttaatcc cgcgtccatc
     6601 aatcatccac tctttgtcca gcgcggaagt gatggttatc cagaggaacg ccacaataaa
     6661 ccagatgcag cacaagacaa acatgcgcgc actcataagg taaccggtcg cttaatttcg
     6721 ctatagcgca tacgaaacaa atcgccgtat cccactaatt ccccgcgaaa aaccagacat
     6781 cggataagat aagcgcaata aatatccggc aggtttcccc accgtttacc atcgcgatct
     6841 ttgagggtgt taatggtgct acccaccacc atcgccactt tacgggtgtg gcaggcatcg
     6901 acaaaagaga gaatcgtctg ctctatttcc gctacatccc cggcagaaaa ttgcagcgcc
     6961 ctgatttcat cttcggttat tgccgggtcc cggctggtgt catccataac atttcctgtc
     7021 tttacgccct atcgtcaggc agacgatagg gcatcgggtt attccaccgc ccactgcggg
     7081 ttttttgcca ctctgctgca atagttctgg taaatcgcca tcgctaacag cgagaagaag
     7141 acagggccgc caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggctga
     7201 ataatggtga agacgttcgc aaatgtcacc accagtacga caaccactgt cgcaatcatt
     7261 gccgacaaat gcgttttgaa aatcacaaat ggtctgtcga gatcctgacg tgctttaaag
     7321 aatgggaaag ccagcgcgag gaacaggtaa ggaagcgtca tagacacgtt cgccatcagc
     7381 gtcagcttgt taaagaacgc ggatgcggta ccgccaccaa acgaaaccag caggatgaag
     7441 acagtaacca acccgcactg catccacatg gcgatagacg gcatccccat cgcattcagg
     7501 cgcgtcatcg gttccggcca caacgctttc ggcgtcccct ggatgatggc tttcagcggt
     7561 gaatagcaaa gcgtaaagaa cgcaccggta taagcgagga acatcgaaag tccggtaata
     7621 cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg acaaatgcag tgcgttaccc
     7681 agcgtcaccc cgaggctctt catcagcaca taggtaatat tgccaaggtt aacggaacca
     7741 ttacttaata cctgctgcca gtttgtgctg acaccccata aaaatattgc cagcgaataa
     7801 ccgattgaaa taacaatagc ggcaaaaaca atacctttgg caaagttctt ttctggattt
     7861 tccgttttat cgaccagacc accaaccgct tcaattccgc cataggcaaa aatagcaaat
     7921 accacaaacg atagcattgc cagaccggac tgataacctg ggttcggtga tgcaaggaaa
     7981 ttaatatcct gcgcgaaatg cccaccattt aataacaaaa tagtaatgct tactaacagc
     8041 aatactaaat tcagacacat tactgcaata ccgcccaccg cagtaatgcg ggcaatttta
     8101 ttaatcccct ttgaagcaac gacggtgacc agaatcatcc atgccactgc cagcagacca
     8161 accacctgcg taggctccag tccagcaata cgccagtgct gggtcatgtc gctaccatag
     8221 aggaatgttg agaacggtac ccaaactttc gcggaggtac tcaccatcca gatgatataa
     8281 gaggaaaacc acataaacgt accaatgaag gcaaaacgtg gtccgacact attattcatc
     8341 caggaataga taccgccttc ttctttgcga taagcagctc ccatttcagc catcattaag
     8401 gcgaatggaa taaagaataa caatgcagaa aatatataaa agggaatcgc actataaccc
     8461 attaagtaat aagccgatgg gctattggca aatccaaata cggaagtaaa gatcatcaat
     8521 atgagtccta tcagactcat cttttttatc gtgtgaggca tgaaaccatc cttcatttca
     8581 gcgcgtgtat aacacgtgcc cactgaactg cactgcgcaa cattgagcag gcagcgtaaa
     8641 aaccaataca gaattgatac ctggatgata tcaaatatcc agtcttaaat tgtggccttt
     8701 aacggaaaaa tgaaaacata tgctacagaa tggcgcttat cacgccattc ttcgctgtta
     8761 attcagtaat ttttcagaat tatgcccggt caacgctaaa ggcgatgact tcagccagtg
     8821 tctccgcgcc cagcgccaac atcaccagac gatcaacacc taatgccacg ccggaacagt
     8881 caggcatacc gactttcaag gcttcaatca gattctggtc aatggggtgc tgcggcaaac
     8941 cgcgcgccgc gcgcttacgg ttatcttgtt caaagcgttg ttgctgctca cgggcatccg
     9001 tcaattcatg gaaaccattc gccagctcaa tacctttata ataaacctca aagcgttcag
     9061 cgacccgatg atcttcggta ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt
     9121 acacaaaggt cggtttttct ttgccaatat ttggctctac gccaaaggta aacagcaatt
     9181 gtagcagcgt gtcgcggtct tcttcggtat cagcaacatt gctcaaatcc agtttcgctg
     9241 cgacttcccg cagttgcgtt ttgtcggcag agagcgggtc aatttccaga taacgcaaga
     9301 aagcttgttg ataagaaagg ctttctgctg ccgggcagtc cagcacctgt tgtaagagat
     9361 cgtccacctc gttcatcaac cggtacatat catagtgcgg tcgataccac tccagcatag
     9421 tgaactcagg gttgtgataa cgccccatct cttcattacg gaagctgcgg cacagctgga
     9481 ataccggccc acaaccggca accagcaggc gtttcatatg gtattccggg ctggtcatta
     9541 accagagatt catcccctgc gaatgcccgg ggccaacgaa acgtgtctca aacgggacca
     9601 aatgaatatc ggttaccgtc gcctggctca tacaaggcgt ctccacctcc agcactccac
     9661 gatcggcaaa gaaacgacgg atctccgcca taatcgccgc gcgttttaat aagttaggaa
     9721 tggatgcgct cggctgccag gatgccgttt cgctcatagt taaatctcca gtttttgaca
     9781 agggcacgaa gtctactcgc aacgcgacgg cgagacaaat tttacgcagg aatcaaacag
     9841 cggtgggcag tgactaaaaa aagcacgatc tgatggttta gtaattaaat taatcatctt
     9901 cagtgataat ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta
     9961 tccatcagac tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta
    10021 actttcaggc acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt
    10081 cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc
    10141 tgcaattgct gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata
    10201 cccgatgcgt agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca
    10261 tgacagcttc gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca
    10321 ggatgtcgtg gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg
    10381 ggggtgccca tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat
    10441 gaaaatcgag cgcacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct
    10501 gttccagacc tctctgcaat tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga
    10561 tattctggtt gatgatggtc atgttcgcgg cctggtagca atgaacatga tggaaggcac
    10621 gctggtgcag atccgtgcta acgcggtcgt tatggctact ggcggtgcgg gtcgcgttta
    10681 tcgttacaac accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca
    10741 cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc tgccaggttc
    10801 cggtatcctg atgaccgaag gctgccgcgg tgaaggcggt attctggtca acaaaaatgg
    10861 ctaccgttat ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa
    10921 caaatatatg gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg
    10981 taaaggcaac accatctcca cgccgcgtgg cgatgtggtt tatctcgacc tgcgtcacct
    11041 cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc gaactggcga aagcgtacgt
    11101 tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg accgcacact acaccatggg
    11161 cggtatcgaa accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga
    11221 atgttcctct gttggtctgc acggtgcaaa ccgtctgggc tccaactccc tggcggaact
    11281 ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa
    11341 tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct
    11401 ggttaaccag gatggcggcg aaaactgggc gaagatccgc gacgaaatgg gcctggcaat
    11461 ggaagaaggt tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct
    11521 ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa
    11581 caccgacctg ctctacacca ttgaactggg ccacggtctg aacgttgctg aatgtatggc
    11641 gcactccgca atggcacgta aagagtcccg cggcgcacac cagcgtctgg acgaaggttg
    11701 caccgagcgt gacgacgtca acttcctcaa acacaccctc gccttccgcg atgctgatgg
    11761 cacgactcgc ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgtgt
    11821 ttacggtggc gaagcggatg cagccgataa ggcggaagca gccaataaga aggagaaggc
    11881 gaatggctga gatgaaaaac ctaaaaattg aggtggtgcg ctataacccg gaagtcgata
    11941 ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg
    12001 cgctgggcta catcaaagac aacctggcac cggacctgag ttaccgctgg tcctgccgta
    12061 tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa
    12121 ccttcctgcg tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg
    12181 aacgcgatct ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt
    12241 acatcatcgg caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga
    12301 tggcaaagta tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc
    12361 cgcagtttgg cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt
    12421 ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga
    12481 acggcgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg
    12541 atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga
    12601 ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca
    12661 atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc
    12721 acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa
    12781 aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc
    12841 attaacctga tcactctggc ggcagctctg ctgcacacca aaacctggtt tgaactggca
    12901 ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa
    12961 agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg
    13021 taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg tattctgggg
    13081 cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt
    13141 gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct
    13201 ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct
    13261 gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaattc acgtgcctgc
    13321 gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtat
    13381 cgttacaatc taacgtatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta
    13441 tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttgc
    13501 tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt
    13561 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
    13621 tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
    13681 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga ggctattgct
    13741 cgaggggaaa tcaagttaag cgatcccaca acaaaatact ggcctgaact taccgctaaa
    13801 cagtggaatg ggatcacact attacatctc gcaacctaca ctgctggcgg cctgccattg
    13861 caggtgccgg atgaggtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
    13921 cctgcatggg cgccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
    13981 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac acgtgtcttc
    14041 cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga aaagaattac
    14101 gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt agatgctgaa
    14161 acttatggtg tgaagtcgac cattgaagat atggcctgct gggtacgaag caatatgaat
    14221 ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca atctcgctac
    14281 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtaaat
    14341 cctgacagca tcattaacgg cagtggcaat aaaattgcac tggcagcaca ccccgtaaaa
    14401 gcgattacgc ccccaactcc tgcagtacgc gcatcatggg tacataaaac aggggcaacc
    14461 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat agtgatgctg
    14521 gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctggcagat tctcaacgct
    14581 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
    14641 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
    14701 cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc cgcgtggcgt
    14761 aacagtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg
    14821 ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga
    14881 tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca
    14941 gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt
    15001 ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc
    15061 gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc
    15121 tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa
    15181 gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc
    15241 caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac cgagcagcac
    15301 aatgccggtg atagccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc
    15361 tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca tcgccgtcac
    15421 ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc caacggccca
    15481 taccacttcc agcagaccag caataactaa gataatccag gacatatcag gctccggaga
    15541 attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag ctgatggttg
    15601 aacggatatt ttgccaggtg gcaggggaat ttcaactatt accttagtga ctatcgcaga
    15661 aaaaacaaac ggcacgacat acctgtcgta ccgttattgc ttattgctat tgctgcgctt
    15721 tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg
    15781 tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg
    15841 tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat
    15901 taataataga caacatcgcc aaaaatgacg gaaatctgat gatttttcgg aagagaagaa
    15961 aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt cgccgaaacc
    16021 gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg
    16081 tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca
    16141 ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat
    16201 ttgtacgaac agcggaacct taaccacagc gccagtagac agggtagccg gtttgccgcc
    16261 agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa
    16321 gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg
    16381 atccagcagc catttagcgt tgtcgccaat tgcttttgca tcagcagaca gctgctcgaa
    16441 agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat
    16501 atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt
    16561 accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac
    16621 gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg
    16681 aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa
    16741 gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc
    16801 aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg
    16861 acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct
    16921 catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcat caggtactta
    16981 cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccgctg gaagaacagc
    17041 acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag
    17101 gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat gccgaaaatc
    17161 agggcaacaa gcgtaactgg cagactgctc ttgagtatgt tgctgcgcat ccggaactgg
    17221 acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt
    17281 tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac agccgtctgc
    17341 cgattgtgat cccggcacgt atcaccgagg cgctggttga acgctttgcc cgttctacgc
    17401 tgcaaatctt gctggtgaat cacatcaatc atgccaatga ggtagatgaa acgttccgtc
    17461 aggcgatggc taaattgcgc cgtgtcggtg tcaccctgct taaccagagc gttctgttac
    17521 gtggtgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgccg
    17581 gcgtaatgcc gtattacctg catgtgctcg ataaagtaca gggcgcggcg cattttatgg
    17641 tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg tcgggttatc
    17701 tggtgccgaa actggcgcga gaaatcggcg gcgaacccag caaaacgccg ctggatctcc
    17761 agctacgcca gcagtaactg attaaacagg gaagttaaac attagcctga tgcgctacgc
    17821 ttatcaggcc tacattgtgc attgaaattg aatggatttg caatgaactg taggccggat
    17881 aaggcgttta cgccgcatcc ggcaagagca acaaacgcaa gtacagcaac ctgtcattaa
    17941 actgggaaat ttaatctttg cccgttgcat aacctttaat tattcatata cattgctgtt
    18001 gttactcttt atatagccaa caccaataat taacctttct tttctgttta tatagagtga
    18061 gtacggctat ttatgttatt ttaacaacta ttactaccaa gcccatttat ctggcttata
    18121 aattaaaatt a
//
LOCUS       DAPHDX010000417        12906 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.guided.130, whole genome shotgun sequence.
ACCESSION   DAPHDX010000417 DAPHDX010000000
VERSION     DAPHDX010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.guided.130"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..293
                     /locus_tag="RH058_004742"
                     /pseudo
     CDS             48..293
                     /locus_tag="RH058_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
     gene            271..663
                     /gene="fliS"
                     /locus_tag="RH058_004743"
     CDS             271..663
                     /gene="fliS"
                     /locus_tag="RH058_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV3140155.1"
                     /translation="MPKAEGRYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            668..979
                     /locus_tag="RH058_004744"
     CDS             668..979
                     /locus_tag="RH058_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDV3140156.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLCAVKARLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            976..2037
                     /locus_tag="RH058_004745"
     CDS             976..2037
                     /locus_tag="RH058_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDV3140157.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            2045..2512
                     /locus_tag="RH058_004746"
     CDS             2045..2512
                     /locus_tag="RH058_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDV3140158.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            2532..3248
                     /locus_tag="RH058_004747"
     CDS             2532..3248
                     /locus_tag="RH058_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDV3140159.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGILLAGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            3261..4124
                     /gene="motA"
                     /locus_tag="RH058_004748"
     CDS             3261..4124
                     /gene="motA"
                     /locus_tag="RH058_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3140160.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            4127..5050
                     /gene="lafU"
                     /locus_tag="RH058_004749"
     CDS             4127..5050
                     /gene="lafU"
                     /locus_tag="RH058_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV3140161.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            5121..6176
                     /gene="dinB"
                     /locus_tag="RH058_004750"
     CDS             5121..6176
                     /gene="dinB"
                     /locus_tag="RH058_004750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV3140162.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            6173..6625
                     /locus_tag="RH058_004751"
     CDS             6173..6625
                     /locus_tag="RH058_004751"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3140163.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            6871..8010
                     /locus_tag="RH058_004752"
     CDS             6871..8010
                     /locus_tag="RH058_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV3140164.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            8007..8621
                     /locus_tag="RH058_004753"
     CDS             8007..8621
                     /locus_tag="RH058_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV3140165.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(8678..10135)
                     /gene="pepD"
                     /locus_tag="RH058_004754"
     CDS             complement(8678..10135)
                     /gene="pepD"
                     /locus_tag="RH058_004754"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV3140166.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            10396..10854
                     /gene="gpt"
                     /locus_tag="RH058_004755"
     CDS             10396..10854
                     /gene="gpt"
                     /locus_tag="RH058_004755"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV3140167.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            10946..12190
                     /gene="frsA"
                     /locus_tag="RH058_004756"
     CDS             10946..12190
                     /gene="frsA"
                     /locus_tag="RH058_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV3140168.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            12248..12649
                     /gene="crl"
                     /locus_tag="RH058_004757"
     CDS             12248..12649
                     /gene="crl"
                     /locus_tag="RH058_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV3140169.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(12688..>12906)
                     /locus_tag="RH058_004758"
     CDS             complement(12688..>12906)
                     /locus_tag="RH058_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV3140170.1"
                     /translation="LRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVD
                     YKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         3216 a         3158 c         3518 g         3014 t
ORIGIN      
        1 gtttattaag tttgttcagg tacgggccgg gatacactgc aacatcaatg catgatcagc
       61 cattattgag tatgtccttg ccgtcgattc cctcttttgt gttgtcggga ttactgttgc
      121 tttgcttgcc gttttcttca tttgccagcg ccaccacatc acatatcact ttcagctacg
      181 ccgcccgcca gcggatgcaa aatcgtgcgc gtttattaaa acagtaccaa acccatctga
      241 aaaaacaggc cagctatatt gtggaaggca atgccgaaag cagaagggcg ctattcgcaa
      301 tataaagaga tcgatctcgc ggcgcgtact gcggcggcat cgcctctgga actggtgctg
      361 gtgctgtttt ctggcctgat ggacgaactg gaacgcgcca aaagccatat tgaagggcgg
      421 cgtttcgaga agaaagccca gagcatcaat aaatgcatcg acattctcaa tgcgctcacc
      481 agttcgctgg agtttgaaac tggcggcgag ctggtggtca acctttcgcg actctatgac
      541 cactgcgttt accgattgta tgaggccagc ggcgaactgt cggcagagaa aatcgatgaa
      601 gtgatgctga tcttaagcaa cctgcgcgaa ggctgggaag ggctttcggg caagctgggt
      661 taagacgatg gaaaaacaac gcaggcaact tttcgcactg gtcgaggcga tgaatgaagc
      721 tctcgacaag caacgctggc gacgccttcc agcactgcat cagcagttga tacgccagtt
      781 tcacgattac gcggcggcgg aaaccgacgc ggcgcagttg tgtgcggtga aggcgcgact
      841 gtacggcgcg tttaatgccc tgattaaacg gcgcgagcag cgcgcagaag tgttgaaggc
      901 gcgtatggaa cagcatcagc ggcatcagga aggtgtgctc gcctactcaa ttgttaattt
      961 actttcggag aagtcatgaa tccggcattg cttgccacgc tcggaacgct tgcggaaacc
     1021 gcttcgttga aggcggatat tttaccgccg gtgagcggtg aaaacgcgcc cgcgtttacc
     1081 ctgccgaaaa tggcggtcgc ggcggtggcg gagcgcgttc atagcgctaa aaccagtcag
     1141 caacaagcga cgcgcccgca ggagaacgat ccggtggcga tgcaggcgct aatggcgctg
     1201 ttacttccgc aacctgccgc gccgcatcag gacacgccgc agccgcgaaa cgtcgcaaca
     1261 tcgccggtta tccagcaatt gacgaaagcg gtggtgcaaa acgcgccgca acgcccgacg
     1321 caacagcagg aactcacgcc gttgccgccg cagttgcagg aactgatcag tcagttgccg
     1381 caggcgaagc ccgaacagca ggccagatta gcgacttacg ccagtgaaga tttacatgcc
     1441 attgcgccga cgcagccgcg cgtctcaaca cagccagctc gcccgaaacc tgaactaacc
     1501 cgtgtgaccg cgcgcccgca ggtcgagcgt aaaacggaaa aagtgccgga cagcgaacca
     1561 gttattgcgc gtgcggtgtt gcaggttaag acgccggagc tggtcagcga tcatcaggag
     1621 attgtcgcca aaccagccac gctttcgatg gatgaactgg gcgaaaaact gacgacgctg
     1681 ttgaaagatc agatccactt tcagctcaac aaacaacagc agatctccac catccgtctc
     1741 gatccaccgt cgcttggcaa gctcgagatc gccgtacaac tcgacaacgg caaactgatg
     1801 gtgcacatcg gcgcgaacca aagtgaagtt tgccgcgcgt tacagcagtt tagcgacgat
     1861 ctccgccagc atctgacggc gcaaaatttt atggaggtga acgtacaggt ttcctccgaa
     1921 gggcagtcgc agcaacaaca acagtcgggc catcagcagg aagaggtgag tgctgcctta
     1981 cagcttgatg atgcgcctca atttcaacag aacgaatccg ttttgatcaa agtgtaaagg
     2041 caatatgaag aaaatcgtgg tggcgggcgt ggtcagcgcc gttctggcgt tggtggtggg
     2101 ggccggagct ggatggggtg tctggcatca ttatgccgga aaaggaaaac cgactgccgc
     2161 cgcgcagacg gaaacggtgg aaaccctgga tgagagcaaa agcgtgtttg tgacgttgcc
     2221 ggaaaccatt gtcacgctgc atgacaataa cggcgcggac cattatttgt cggcggaatt
     2281 agtgatggtg gtcgccagtg acaaagaagc ggaaaaaata aaacatcagg agccgctgta
     2341 tcaaagtatt gcggttgaat gcctgacgga aatgaaattt gaagatttgc gtggcatgaa
     2401 aatatccgcc atccgcaaac tcatttctga cgcgttaaag aaagatcttc agcgtcgcaa
     2461 aatgagcgca ccgtataagg atttgctggt gaaaaaagtg gtcttccagt aatcaccagg
     2521 cggatataaa catgctacag gatgtattcg agtcagaaga atttgctgcc gcgccggtat
     2581 taaccccgca gcaggagagc cactacttgc aggcctattt accgttggtg cgtaaagtgg
     2641 tgcggcaact tgccccgcag tgtaattgca ttattgatcg tcaggatatg gaacaaacgg
     2701 cgttaatggg gctgcttaat gccatccgcc gctacgggct gccggacgaa ggttttgcgg
     2761 gctatgctgt gcaccgtatt cgcggcgcga tcctggatga gctgcgcgct ctcgactggc
     2821 gaccgcgcca gctgcgacag aaatatcatc agatgaaaga tctgattcgc gaaacgcgca
     2881 aaaaactggg gcacgaaccg gagtggtcag aactggccgt ggaagggatc tcgcatgaag
     2941 attatctcga atatcagcaa ctggaagggg cggaaacgct cgccagcctg gacgaactgc
     3001 tgaacggcga agggcctggc attctgcttg ctggcaggga gctggaagat cagtttgtca
     3061 cccagcaaat gttgcaacag gcgctttcac aattaagcga aaaagagcaa ctgatcctct
     3121 ccatgtatta ccagcatgag atgaatctca aagagattgc gctggtatta gggctaaccg
     3181 aggcccgaat ttgtcaactg aataaacaga tcgcgcagaa aattcgtgat tttgtctatc
     3241 cgaattaaag tgaagaaatc atgcaaaaaa tattaggttt actggtggtg ctcggctgcg
     3301 tatttggcgg ttattttgaa gcgggcgggc aattagtggc tatctggcaa ccggcggaaa
     3361 tgatcattat tttgggtgcc ggtttcgggg cgatgattat tggtaacccg aaacatgtgc
     3421 tgaaagagat tgcccatcaa attaaaggcg tgatcagtaa aaaacagctc gggccggagt
     3481 ttcagcgtca gctattaatg tgcctgtatg agttgctcga aatggtgcaa aacggtggtc
     3541 tgcgcatgct ggatcaacat attgagcaac cggaagagag caccattttc cagaaatatc
     3601 cgctggtgtt aacccagaag cggctggtga cctttattgc cgataacttt cgcctgatgg
     3661 cgatgggtaa aatcgacgcc catgaactgg aaggcattct cgatcaggaa ctggacactg
     3721 cggaagagtc gctgctcacg ccgtcgcgct cgctgcaacg taccgccgaa gcgatgccgg
     3781 gatttggtat ttgtgcagca gtgctgggga tcatcatcac catgcagtcg attgacggct
     3841 ctattgccct gatcggcctg aaagtggcgg cagcgctggt ggggacgttt ttaggcgtct
     3901 ttatctgcta ctgcctgatg gacccgctcg ccaacgccat ggaacaacag gcgcgggcag
     3961 aacattcttt gctggaatgc gtgcgcactg tgctggtggc gcaggcaggc ggcaaaccga
     4021 cgctgctggc ggtggatgcc gggcgtaagc tgctgcatct ggcgtccaaa ccgaccttcg
     4081 ccaacctcga cgcgtgggtg aatgcgatgc tggagcaaga ataagcatga gaaagacggg
     4141 caaccgccgc gagcgtgggg caaaaaccac catcgtccgg cggcagatca aaaagaatca
     4201 tgccgggcat catggcgggg cgtggaaggt ggcgttcgcc gactttactt tggcaatgat
     4261 ggcgctgttt atgaccctgt ggattgtcaa cagcgtcagt aaatccgagc gtgaaagcat
     4321 tatcgccgcg ctgcacgggc agtcgatttt taacggtggt gggctgtcgc cgttaaataa
     4381 aatcagcccg tctcatccgc caaaacctgc caccgtggcg gtgccggaag aaacggagaa
     4441 aaaagcgcgt gatgtgaatg agaaaacggc gctgctgaag aagaaatccg ccaccgagct
     4501 tggcgagctg gcaaccagta tcaccaccat tgcccgtgat gcgcatatgg aagcgaatct
     4561 ggagatggag attgttcccc agggattacg cgtgctgatt aaagacgacc agaatcgcaa
     4621 tatgttcgaa cgcggcagcg cgcagattat gccgttcttt aaaacgctgc tggtggagct
     4681 cgcgccagtt ttcgactcgc tcgataacaa aattattatt accgggcata ccgatgcgat
     4741 ggcttacaaa aacaatatct acaacaactg gaacctttcg ggtgaccgcg cgctttcggc
     4801 tcgtcgggtg ctggaagagg ccggaatgcc ggaagataaa gtgatgcagg taagcgcaat
     4861 ggcggaccag atgctgctgg attccaaaaa tccgcaaagc gcgggcaacc ggcgcattga
     4921 gattatggtg ctgaccaaaa gtgcgtccga tacgctgtat caatactttg gtcagcatgg
     4981 ggataaagtg gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc tttcgcagcg
     5041 aacgcgttaa atgctgaatc tttacgcatt tctcaaaccc tgaaatcact gtatacttta
     5101 ccagtgttga gaggtgagta atgcgtaaaa tcattcatgt ggatatggac tgctttttcg
     5161 cagcggtgga gatgcgcgac aatcccgccc tgcgcgatat ccctattgct attggcggca
     5221 gccgcgaacg tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac
     5281 gtagcgctat gccgacaggg atggcgctca aattatgccc gcatctcacc ttgcttccgg
     5341 ggcgctttga cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc tcgcgctaca
     5401 cctcgcgtat tgaaccattg tcactggatg aggcttatct cgacgtcacc gatagcgtcc
     5461 attgccacgg ttctgcgacc ctcatcgccc aggaaatccg ccagacgatt ttcagcgagc
     5521 tgcaactgac agcgtctgcg ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg
     5581 acatgaataa acccaacggt cagtttgtga ttacgccggc agaagttccg gcatttttac
     5641 aaaccttacc gctggcaaaa atccccggcg tcggcaaagt ctcggcggca aaactggaag
     5701 cgatggggct gcggacctgc ggtgatgtac aaaagtgtga tctggtgata ctgcttaaac
     5761 gctttggcaa atttggccgc attttgtggg agcgtagtca ggggattgac gagcgcgacg
     5821 ttaacagcga acggttgcga aaatccgtcg gcgtggaacg cacgatggcg gaagatatcc
     5881 accactggtc tgaatgtgaa gcgattatcg agcggctgta tccggaactt gaacgccgtc
     5941 tggcaaaggt aaaacctgat ttactgattg cccgccaggg ggtgaaatta aagttcgacg
     6001 attttcagca aaccactcag gagcacgtct ggccgcggct gaataaagct gacttaatcg
     6061 ccaccgcgcg taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcga ctggtggggc
     6121 tgcatgtgac gttgcttgac ccgcaaatgg aaagacaact ggtgctggga ttatgaataa
     6181 catacaaata agagactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag
     6241 agcggttacg atgactgcca gtcagcatta ttcaccacaa caaattgccg cctgggcgca
     6301 gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat
     6361 taatgcaaaa ctggtcggct ttattacctg cgttgaacac tatatcgata tgttatttgt
     6421 tgaccctgaa tacacccgcc gtggggttgc cagcgctttg ttaaaacctt tgattaagtc
     6481 tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa cccttttttg aacgttatgg
     6541 ttttcagaca gttaagcagc aacgcgttga atgtcgggga gagtggttta ttaattttta
     6601 tatgcgttat aaacagcaac attaaatcca gcttgcaatg aaaataacgc aagcctagta
     6661 tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca
     6721 atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc
     6781 catttcctga ttttttaatt gttactctca ataattcaag tcgcaacaca tgcaacttga
     6841 agtatgacga gtctatttac ggaatggtat atgggcaatt atattcgtcc cttatccgat
     6901 gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat ccaacaatta
     6961 cacaccacgg caaatttacc caacatgcag cgcgtagtgg ggatgccaga tttacatccc
     7021 ggacgcggct atccgattgg cgcagcgttc ttctctgttg gtcgttttta tccggcactg
     7081 gtcggcaatg atatcggctg cggtatggcg ctatggcaaa cagatattct cactcgcaaa
     7141 tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc tgaagaaagc
     7201 tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc
     7261 tctatcggtg gcggcaatca tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct
     7321 gaactgtttg cactggcagg tctggatgca caacatctgc aactgttggt tcatagcggc
     7381 tcgcgggggg tggggcagtc tattttacag cggcatattg cctcgttctc gcatcatggt
     7441 ttgcctcaag gcagtgacga cgcgctacgg tatattgcgg aacatgacga tgcgctggcg
     7501 tttgcgtgta ttaatcgcca gatgatcgct ttgcgcataa tgcaacaagt taaggccacg
     7561 ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa
     7621 cagggctggt tgcatcgtaa aggtgccacg ccggatgaca acggtctggt gattattccc
     7681 ggttcacgtg gtgattactc ctggattgta cagcctgttg cgaacgaaaa aacgctccac
     7741 tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca
     7801 gcgaaataca cggcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc
     7861 gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga aagcgtggtg
     7921 caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg
     7981 ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctca gcgcaggggc
     8041 cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc
     8101 gacaagacgt cgcggtaacg gtgttggaaa cagaaacggg ccgctactct gacacgctgc
     8161 gttcagctct ggtttctctg gatggcgata acgcatgggc attaagcgaa agctggtgcg
     8221 gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc aaaaactggt
     8281 ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg
     8341 agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
     8401 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgt agtcagcatg
     8461 ctaacaagcg gctggcacga ttgctgattg cctggaagct ggagcagcag caacaggaaa
     8521 atagcgcggt gctgaaatcg cagcggcgaa tgttccatca ccagattgaa cgtggcaacc
     8581 cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
     8641 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
     8701 gcagttcagt cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
     8761 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
     8821 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggt gttttgttga
     8881 acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
     8941 agccaggata tgcgcctttc gcttcggttt tcgcgccagc tagtttaccc agcgaatcca
     9001 gcatgctcac cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt
     9061 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
     9121 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
     9181 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
     9241 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
     9301 gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
     9361 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcagatcc agttcttccg
     9421 catgacccgc caggaagcgc accagtagtt tgttggcatt acccaggcca acgtggattt
     9481 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
     9541 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
     9601 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
     9661 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
     9721 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac
     9781 caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
     9841 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
     9901 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
     9961 tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
    10021 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
    10081 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
    10141 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
    10201 gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
    10261 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    10321 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    10381 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    10441 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    10501 agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    10561 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctaaaa
    10621 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    10681 actgcggttg cgattcgtga aatgtatcca aaagcacatt ttgtgactat cttcgcaaaa
    10741 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
    10801 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    10861 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
    10921 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    10981 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    11041 accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    11101 ccgtctgatg tggatctggc gcggcattga cccacgtgaa atcctcgacg tccaggcacg
    11161 tattgtgatg agcgatgctg aacgtaccga tgatgattta tacgataccg tgattggcta
    11221 ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc
    11281 ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta
    11341 caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
    11401 atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga
    11461 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
    11521 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
    11581 cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc
    11641 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
    11701 cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
    11761 ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc
    11821 ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca
    11881 ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga
    11941 tgacgcgttg cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg
    12001 tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga
    12061 ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa
    12121 cccggtgtat cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg
    12181 cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat cacaacagga
    12241 gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc
    12301 actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct
    12361 ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg gctggtggat
    12421 ggagctggaa gcgcaggaat cccgttttac ctacagttac cagtttggtc tgttcgataa
    12481 agcaggcgac tggaagagtg ttccggtaaa agacactgaa gtggttgaac gactggagca
    12541 caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct
    12601 ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc
    12661 cggatgcatc acatccggca ataatcatta aaactgatag gtcatgccaa ccgcgacaat
    12721 atcatcatta ttaatattca atttgttatc gctatccagt tggttgattt tataatcaac
    12781 aaacgctgac atatttttgt tgaaataata tgtagccccg acatcgatat aattgaccag
    12841 atcttcatca ccgatacctt caatatcttt ccctttcgat aagacataac ccagcgatgg
    12901 acgcag
//
LOCUS       DAPHDX010000418         7287 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.guided.181, whole genome shotgun sequence.
ACCESSION   DAPHDX010000418 DAPHDX010000000
VERSION     DAPHDX010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.guided.181"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            241..1044
                     /locus_tag="RH058_004759"
                     /note="aph(3'')-Ib"
     CDS             241..1044
                     /locus_tag="RH058_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV3140171.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1044..1880
                     /locus_tag="RH058_004760"
     CDS             1044..1880
                     /locus_tag="RH058_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDV3140172.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            2216..3031
                     /locus_tag="RH058_004761"
     CDS             2216..3031
                     /locus_tag="RH058_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV3140173.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(3185..4060)
                     /locus_tag="RH058_004762"
     CDS             complement(3185..4060)
                     /locus_tag="RH058_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV3140174.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(4130..7015)
                     /locus_tag="RH058_004763"
     CDS             complement(4130..7015)
                     /locus_tag="RH058_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV3140175.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            7141..>7287
                     /locus_tag="RH058_004764"
     CDS             7141..>7287
                     /locus_tag="RH058_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3140176.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDGTVAKLAM
                     RQPFV"
BASE COUNT         1557 a         2009 c         2094 g         1627 t
ORIGIN      
        1 gacaagatgt gtatctacct taacttaatg attttgataa aaatcattag gggattcatc
       61 agcactcggc tggaaatcgc cggtggcatt tgagaaaaaa gccgcttaaa atgagagata
      121 gaccggaaca caaccggtgc aagaccatgg aaagcatgtt ctcggactta cgtagcaact
      181 cgtttctttt cgcaggttga gccacctccg cgcttcatca gaaaactgaa ggaacctcca
      241 ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc tgtcagaggc
      301 ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca
      361 cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt
      421 cgaggtgtgg cttgccccga ggtgatcaac tggcaggagg aacaggaggg tgcatgcttg
      481 gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga tttgctcaaa
      541 gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt tgatcaatgt
      601 ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat
      661 gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct cgatcttttg
      721 gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga tatggttgtt
      781 tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct tcaatgcacg
      841 ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt ggcactcatg
      901 attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc
      961 ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt ctatctgcga
     1021 ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct cattggcacg
     1081 tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa gtttcattgc
     1141 cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt gctgatgaac
     1201 tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg ttgctcggtc
     1261 gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct cacatcgttg
     1321 ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg
     1381 ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc tttgcagctt
     1441 tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac gtccacgcgg
     1501 cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta catggcgatc
     1561 tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat cccgtcggtc
     1621 tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct gacagagacg
     1681 acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct cgtgcgctgg
     1741 acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt
     1801 ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg atcaagcagg
     1861 tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga acacatcaat
     1921 cttaccggag aatatcgttg gccaaagcct tagcgtagga tttcgccctc tcccgcaaac
     1981 gaccccttta ttgtaggtgg gccagttggt gattttgaac ttttgctttg ccacggaacg
     2041 gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat
     2101 tcaacaaatc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2161 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2221 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaacc tggatgctga
     2281 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2341 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2401 caatgatgtt acagatgaga tggtcagact aaactggctg acggcattta tgcctcttcc
     2461 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgctccc
     2521 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2581 tgcgctggca gcgttcctgc gccggttgca ttcgattcct gttagtaatt gtccttttaa
     2641 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2701 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2761 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2821 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2881 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgaat tttctccttc
     2941 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3001 gtttcatttg atgctcgatg agtttttctg atagttagtc tttgtagaga cacaaacctg
     3061 aaattccgga caccgcgctt caggacatct ccctggacgc cgatatctgg cagtatccgg
     3121 acggtactat cgaacgccgg gccaacggta ctcccctgcc ctttatgact gacgaccggc
     3181 tatttcaccc acgcacttct ccatgaaatt tagcatcgtc gcggttaatg tcgaaaattt
     3241 gccacctgac gtggttggca tcgagggtga aaactgccgt ggcgagggtg ttctcctcat
     3301 ccggatcgtc ggcagccagc cggtaaattg gcagttctgc gtcatgggta tctgagagaa
     3361 tggtttttgc cgccgcggcg tcaaacggag gttgcgtttc ccgccactgc acgaggcgta
     3421 actgccgcga cgctgaactg tcggtaacga tctgctcaac gccagccagt gccgcatgca
     3481 ccagatggtt ggcatgcccg gttaccgtcg tcaggggtag aacggaacag ccttgaccgg
     3541 tggcctcaac gctgaacagc cgcgcatcgc ccagctgccc gagcgtatgg tgaaacgccc
     3601 cggatcgcgg ctggccggta agtatagtga tggcctcgtc gagggtgttc gcattcagcg
     3661 aggcgcgagc caggatctga cgcggtaatc cctgcggacg atacactgca cggatgttat
     3721 tcaccgtgtt aacgatacct ttttcattta ccgcaaaggt gtggccgcaa atcgaccccg
     3781 gataggcaaa actcgtaaag ctcagaccgc gactcggagt aatggtagcc agcaagcatt
     3841 catcccgtaa ctgaggaaaa ccgtcttcat tatgcgcgat aatccgctcg cctgcggcgt
     3901 ttaccccggc caccgtcgta cagccatccg acgttgagcg caccagatcg ccacggcagt
     3961 tccaggcaaa gacctgatca acagtggcct ccagtccctg cgccagcccc tccagctcct
     4021 gccagatgtg cggataatgc cgctgcacct cggcgcgcat taaccgggtc tcaggggatg
     4081 cctgcagggt catcacgggg tcgtttgcgg gagggggcgg aatcctacgc taaggctttg
     4141 gccagcgata ttctccggtg agattgatgt gttcccatcc gagcggcgaa acatgggcca
     4201 agagatcggg cgatagcagc tttccatcgc gtttctggtt tgcaacgacc tcgccgagct
     4261 tcatggtgtt ccagaagatg atgatggcgg cgagcagatt catgccggcg atgcggtaat
     4321 gctggccttc ggcggaacgg tcgcggattt caccgcggcg gtggaagctg attgcccgct
     4381 tcagcgcatg atgagcttcg cctttgttga gcccgatctg ggcacgccgt tggagttcgg
     4441 catccagaat ccagtcgatc atgaacaggg tgcgctcgac gcgaccgact tcccgcaggg
     4501 ctgtcgcgag ctcgttctgc cgcggatagg aggcgagttt ccgcagaatc tggcttggcg
     4561 cgacggtccc ggcagcaatg gtggcggcga tgcgcaggat gtcgggccaa ttgcgctcga
     4621 tcatggcttg gttgaccttt ccgccgatca acgctcgcag gtgcgccggg gcggccgacg
     4681 gattgaacgc gtagagccgt ttggatggca ggtcgcggat gcgcggagcg aaccggtagc
     4741 cgagaatggc acatgcggca aagacgtgat cggtgaagcc gcccgtgtcg gtgaactgct
     4801 cgcggatatg gcgtccagca tcgttcatca gcaggccatc gaggatgtaa ggcgcttcgc
     4861 ttgccgttgc aggaatcacc tgggttgcga acggcgcata ttggtcggag acgtggctat
     4921 aggctttcag gcccggggta ttgccatatt tcgcgttgac caggttcatg gcctcacctt
     4981 gctctgtagc gacgaagaac tgtccgtcgc tcgaagccga cgtgcccatg ccccagaacc
     5041 gggccatggg taacgctgcc tgtgcctcga ccaccatggc cagcgcccgg tcataggctt
     5101 cgccctcgac atgccaccgt ccaatgcgga tcaattccca gaaggtgtgg gtgtttgtcg
     5161 catccgccat tttgcgcaag ccgaggttga tcccttccgc caagataacg ttcattagcc
     5221 cgatccggtc agcgcagggt gctcctgtgc gcagatgggt gaacgcttcg gtgaagccgg
     5281 tcgccgcatc cacctccagc aggagatcgg tgatgcgcgt gggcgggatc tgcttgtaga
     5341 gatcgagcac cagatcttcg gcgcctgtcg gcgcggcggc ttcgagtttc tcgatatgca
     5401 gaacgccgtt ttcaatcgac ccgcccggga tcgtgcctgc gcgagcggca cggccaagct
     5461 cgcgcaaccg catgtcgagg cgagcttgcc ggtctgccag ccattcctcc ggccgcaatg
     5521 gcacagcgag acgaccgcct tccgcgatgg attgtgccgg aacgagtgcg tgtttcagat
     5581 cgccatagcg ccgggaccta gtaagccaga catctccgga gcggaacgca tcgcgcagat
     5641 ggaacagcac cgcgatctcc cataggcgag cgtcgccagc cctctgggcc cgaaggtggc
     5701 gatgccattt cgagctgggc cgcaagaagc tggtcatcgc ggcatcgttc aaaccggtac
     5761 gaagggccgt caccgcttcc agaagcggca gtgcaacggg cgcagctcgc agatcgagca
     5821 ggcgcaacat gcgtggagcg tatcggcgga agcggtgata accgtcgagc acatgattga
     5881 gcggatcgtc ggccatggtg gcggtcagcc tggttgccat tgcaacaagg gtttttaagc
     5941 cgtcccaccc tgacccactc gcgatgacat cgcccagcgg ctggccatca tcctgtgcat
     6001 cgaccagggc gcccccgatc tcggcgaagg atttcagggt gtcacgcacc acccccgctt
     6061 cgtctgcgac ctttgcatgg caaatacgct ccgaagcacg gtagagacgg ccgacgatcc
     6121 ggtcgtgggt ttcgaccact gcgtcggcca acatcgcctg ccattccgag acgcaaacag
     6181 ccaagatcgc aagccgcctg tcctccggga gatcgcgcat gccgtcggca taataccgtt
     6241 caccctgcct gcgcagacga gtcacccgat gggcaggaac gccggcaagc agatcctcgg
     6301 ggagatcgat gcgttgcaga tattcgagcc ggtcgagcag ccggttggcc gacgaagagt
     6361 tcgagccagg ctcgaactgg cgcagccaca caaaacgggt cacccgatca tcagccgtct
     6421 cctcgagcaa tgccagcaac tgttctcgga tcgacatagg cagccgactg gcgatcctcg
     6481 tctcgatgcg tcgctcggca tcgacgagag ccgcggcaca aagccgctcg atcgtggatg
     6541 tcgcgggaag gacagtgcgg gtgcgtcggc actcggctac gaagcgacgg gcgatatcct
     6601 cgttcgacac cgccatctcg gcttctcgga acaaccattc cttcagctcg ctcgcaccac
     6661 gtccggagaa ggtgcggaag ccgtagagcc cccgtaactc ggcaagatgc tcgtgccgtg
     6721 tttcctcgcg ggcagcatag tctacgagat cgtcggcacc caggccaagc tgcgctccga
     6781 taaattcgat gacctctgca gggatcagtt cgcctggagc cagcacccgg ccgggatagc
     6841 gcaggacaca caattgcagg gcgaagccga acctgttgtg agcgcgccga cgcagcctga
     6901 tatgcccaag gtcttcatca ctcagcgtat agtgcttgag caaatccgtc tgtgaagtcg
     6961 gcaagcgcaa cagcgcgtct ttctgccgat cggttagagt gacgcgacgc ggcatacatg
     7021 ttcctttttc aaaatctgat agcgttcaag acgctttgtt tatgaagctg gttgagatac
     7081 acttccagag gtcaatgcaa tcgtggccga agcgccgcct caaaccaacg tttgtgatac
     7141 atgctgatcg gatatgcccg cgtctccaaa gccgatggct cgcagtctct cgacctgcag
     7201 cacgacgcct tgcgcgccgc aggtgtcgaa cgggacaata tctatgatgg cactgttgca
     7261 aagttagcga tgaggcagcc ttttgtc
//
LOCUS       DAPHDX010000419         2891 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.guided.254, whole genome shotgun sequence.
ACCESSION   DAPHDX010000419 DAPHDX010000000
VERSION     DAPHDX010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.guided.254"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            306..1451
                     /locus_tag="RH058_004765"
     CDS             306..1451
                     /locus_tag="RH058_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV3140177.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1545..2078
                     /locus_tag="RH058_004766"
     CDS             1545..2078
                     /locus_tag="RH058_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV3140178.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(2075..2392)
                     /gene="sugE"
                     /locus_tag="RH058_004767"
     CDS             complement(2075..2392)
                     /gene="sugE"
                     /locus_tag="RH058_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3140179.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
BASE COUNT          766 a          726 c          711 g          688 t
ORIGIN      
        1 cagccgagaa gaatttcccc gcttattcgc accttcctta agtatcattg cagcaaagat
       61 gaaatcaatg atttatcaaa aatgattgaa aggtggttgt aaataatgtt acaatgtgtg
      121 agaagcagtc taaattcttc gtgaaatagt gatttttgaa gctaataaaa aacacacgtg
      181 gaatttagga aaaacttata tctgctgcta aatttaaccg tttgtcaaca cggtgcaaat
      241 caaacacact gattgcgtct gacgggcccg gacacctttt tgcttttaat tacggaactg
      301 atttcatgat gaaaaaatcg ttatgctgcg ctctgctgct gacagcctct ttctccacat
      361 ttgctgccgc aaaaacagaa caacagattg ccgatatcgt taatcgcacc atcaccccgt
      421 tgatgcagga gcaggctatt ccgggtatgg ccgttgccgt tatctaccag ggaaaaccct
      481 attatttcac ctggggtaaa gccgatatcg ccaataacca cccagtcacg cagcaaacgc
      541 tgtttgagct aggatcggtt agtaagacgt ttaacggcgt gttgggcggc gatgctatcg
      601 cccgcggcga aattaagctc agcgatccgg tcacgaaata ctggccagaa ctgacaggca
      661 aacagtggca gggtatccgc ctgctgcact tagccaccta tacggcaggc ggcctaccgc
      721 tgcagatccc cgatgacgtt agggataaag ccgcattact gcatttttat caaaactggc
      781 agccgcaatg gactccgggc gctaagcgac tttacgctaa ctccagcatt ggtctgtttg
      841 gcgcgctggc ggtgaaaccc tcaggaatga gttacgaaga ggcaatgacc agacgcgtcc
      901 tgcaaccatt aaaactggcg catacctgga ttacggttcc gcagaacgaa caaaaagatt
      961 atgcctgggg ctatcgcgaa gggaagcccg tacacgtttc tccgggacaa cttgacgccg
     1021 aagcctatgg cgtgaaatcc agcgttattg atatggcccg ctgggttcag gccaacatgg
     1081 atgccagcca cgttcaggag aaaacgctcc agcagggcat tgcgcttgcg cagtctcgct
     1141 actggcgtat tggcgatatg taccagggat taggctggga gatgctgaac tggccgctga
     1201 aagctgattc gatcatcaac ggcagcgaca gcaaagtggc attggcagcg cttcccgccg
     1261 ttgaggtaaa cccgcccgcc cccgcagtga aagcctcatg ggtgcataaa acgggctcca
     1321 ctggtggatt tggcagctac gtagccttcg ttccagaaaa aaaccttggc atcgtgatgc
     1381 tggcaaacaa aagctatcct aaccctgtcc gtgtcgaggc ggcctggcgc attcttgaaa
     1441 agctgcaata actgacgatg aggcccagga tattgggcct cctttctttc tctttttttc
     1501 ctgttgtcat ctacacttaa caaaaataca gcaaggaaaa tcccatgcgc attttgcccg
     1561 tcgttgctgc agttacggct gcattcctgg ttgtcgcgtg tagctccccg acaccgccga
     1621 aaggcgttac cgtggtaaat aactttgatg ccaaacgcta tctgggaacc tggtatgaaa
     1681 ttgcgcgctt cgaccatcgt ttcgagcgcg gattggataa agtgaccgca acatacagct
     1741 tgcgcgacga cggcggcatc aacgttatta acaagggcta taaccctgac agggagatgt
     1801 ggcagaaaac ggaagggaaa gcctatttca ccggcgaccc aagcagagcc gcgcttaagg
     1861 tttctttttt cggccccttc tatggcgggt ataacgtaat tgcactcgac cgggaatatc
     1921 gtcacgcgct ggtttgtggt ccggatcgcg actacctgtg gatcctttca cggaccccta
     1981 ctatttcaga tgaaatgaaa cagcaaatgt tagccatcgc gacccgggaa gggtttgaag
     2041 tgaataaact gatttgggtg aaacagcctg gcgcttagtg agtgctcagc ttcagaccaa
     2101 taatgccagc aacgatcagc ccaaggctca gcaaacgtgc cgggctggca gactcaccca
     2161 gcagcaaaat ccctgtaatg gccgccccaa cagcgccaat accggtccag accgcataag
     2221 cggttcctac aggcaacgtg cgcattgccc aagagagcat ggcgatactg acgatcatcg
     2281 ccgcaatagt gataatgctt ggcgtaagac gcgtaaaacc gtgggtgtat ttcaggccaa
     2341 tcgcccagac aacttcgagc aaacctgcaa ttaataaaac gatccaggac atatcaggct
     2401 ccagaacaat ggggccgtcc ccggtgaaag aagcgtttgc aggtcgtcct gcaaagctaa
     2461 tgtgtgaaat ggcatttttg cccggaagaa aatgaatttc aaccttttta ttcaccgcct
     2521 gctaaaagca agaattaagc ataattagcg gcgtagttcc cgcatttggc actcatcgga
     2581 gttctctatg atgaagggct ttctgaaacc agaaagtcga ttgcccctct tctgaatgcg
     2641 aaacgtttat gcttaaaatt ttagtgattg accggtgcca ctttacccgc acagggatag
     2701 aggccttgct taatcattct ggcaggttca gctcctcatt tctggtatca ggaatcaata
     2761 atctcctgtt agcaaaagag catattttgc aatggaaacc gcatctggtg atcgcggatt
     2821 tatacagttt tattagcgag acgcactcca gtccgccaat taaacctttt tttatgagtt
     2881 gtggcgtcat c
//
LOCUS       DAPHDX010000420         1426 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16540
            SAMN37211190-rid19353943.guided.310, whole genome shotgun sequence.
ACCESSION   DAPHDX010000420 DAPHDX010000000
VERSION     DAPHDX010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211190
            Sequence Read Archive: SRR25824662
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875386.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875386.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 05:55:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,768
            CDSs (total)                      :: 4,687
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 30 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211190-rid19353943.guided.310"
                     /isolate="ARS-CC16540"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            92..1297
                     /gene="tet(B)"
                     /locus_tag="RH058_004768"
     CDS             92..1297
                     /gene="tet(B)"
                     /locus_tag="RH058_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV3140180.1"
                     /translation="MNSSTKIALVITLFDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          346 a          264 c          325 g          491 t
ORIGIN      
        1 atctagacat cattaattcc taatttttgt tgacactcta tcattgatag agttatttta
       61 ccactcccta tcagtgatag agaaaagtga aatgaatagt tcgacaaaga tcgcattggt
      121 aattacgtta ttcgatgcca tggggattgg ccttatcatg ccagtcttgc caacgttatt
      181 acgtgaattt attgcttcgg aagatatcgc taaccacttt ggcgtattgc ttgcacttta
      241 tgcgttaatg caggttatct ttgctccttg gcttggaaaa atgtctgacc gatttggtcg
      301 gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg gattacttat tgctggcttt
      361 ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt tcagggatca caggagctac
      421 tggggctgtc gcggcatcgg tcattgccga taccacctca gcttctcaac gcgtgaagtg
      481 gttcggttgg ttaggggcaa gttttgggct tggtttaata gcggggccta ttattggtgg
      541 ttttgcagga gagatttcac cgcatagtcc cttttttatc gctgcgttgc taaatattgt
      601 cactttcctt gtggttatgt tttggttccg tgaaaccaaa aatacacgtg ataatacaga
      661 taccgaagta ggggttgaga cgcaatcgaa ttcggtatac atcactttat ttaaaacgat
      721 gcccattttg ttgattattt atttttcagc gcaattgata ggccaaattc ccgcaacggt
      781 gtgggtgcta tttaccgaaa atcgttttgg atggaatagc atgatggttg gcttttcatt
      841 agcgggtctt ggtcttttac actcagtatt ccaagccttt gtggcaggaa gaatagccac
      901 taaatggggc gaaaaaacgg cagtactgct cggatttatt gcagatagta gtgcatttgc
      961 ctttttagcg tttatatctg aaggttggtt agttttccct gttttaattt tattggctgg
     1021 tggtgggatc gctttacctg cattacaggg agtgatgtct atccaaacaa agagtcatca
     1081 gcaaggtgct ttacagggat tattggtgag ccttaccaat gcaaccggtg ttattggccc
     1141 attactgttt gctgttattt ataatcattc actaccaatt tgggatggct ggatttggat
     1201 tattggttta gcgttttact gtattattat cctgctatcg atgaccttca tgttaacccc
     1261 tcaagctcag gggagtaaac aggagacaag tgcttagtta tttcgtcacc aaatgatgtt
     1321 attccgcgaa atataatgac cctcttgata acccaagagg gcatttttta cgataaagaa
     1381 gatttagctt caaataaaac ctatctattt tatttatctt tcaagc
//
